LOCUS       DASYEG010000001         8886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3769028, whole genome shotgun sequence.
ACCESSION   DASYEG010000001 DASYEG010000000
VERSION     DASYEG010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 8886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8886
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3769028"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="VGM72_00005"
     CDS             complement(<1..1006)
                     /locus_tag="VGM72_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY4263671.1"
                     /translation="MAHARSLAEQLGEPVAGGGLLHRRFFLQSGAALAGIAAANPARA
                     ESIGSLSPPSMLKPGSGFTAYGMPSHWRENIKRILVANAPPGREVTGSSRTPLQLLEG
                     AITPAGLHYVRIHNGVPDIDPDKHRLTIHGMVKQPLVFGMDALMRYPMQSEIRFMECA
                     GNSGGMNAPQAPQQSAGDIHGLLSGSEWTGVKLSTLLDEAGLDPKASWILAEGADGAG
                     MSRSVPVWKAMDDAMIALFQNGEPIRPEQGFPMRLLLPGFEGNTNVKWLRRLKVTDTP
                     TYTKDETSRYTELMPDGKARQFMLQMEPKSVILKPSFGIAMQGPGYYEISGLAWSGLG
                     R"
     gene            complement(1123..1596)
                     /locus_tag="VGM72_00010"
     CDS             complement(1123..1596)
                     /locus_tag="VGM72_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263672.1"
                     /translation="MSLGLLVGRFASFVSEQPLPPEPAGIVEDFPSDLMRSRDAFGHH
                     LVTLLLIARCDGECAVQEREVILDHCLDRAKLSGLELSLMEKTALSDYLHAFRPSRAQ
                     FGPALKRLHHDSHGDIVSLVAAAQRVVDADGVRRPREIKFLEDLSRDLACLDAIS"
     gene            1763..3226
                     /gene="zwf"
                     /locus_tag="VGM72_00015"
     CDS             1763..3226
                     /gene="zwf"
                     /locus_tag="VGM72_00015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY4263673.1"
                     /translation="MAKIPPGVGPKYPQVVVLVGATGDLSRRKLLPGLFHLSRAGFIP
                     GCRIIGVSLDNIDADGFRAIAREALDQFSTRKMSESDWSNFAQSLDYVPLGAGATGLK
                     AAVDKAESLLGRECRRLHYLSVPPAAALSAVRLLGEADLVERSQIVMEKPFGTDLASA
                     VTLNARLHEVFAEHQIFRIDHFLGKEPAQNILAFRFGNGLFEPIWNRNFIDHVQIDVP
                     ETLGLGKRAGFYEQTGAYRDMVVTHLFQILGFMAMEPPTSLEPAPISEEKNKVFRSMV
                     PIEPKNVVRGQYIGYRAEEGVDPESDTETFVALKCRIDNWRWAGVPFFLRTGKRLAEG
                     QRIISIAFREPPKSMFPVGSGVGAQGPDHLTFDLADASKMSLSFYGKRPGPGMRLDKL
                     SLQFAMHDTGLIGDVLEAYERLILDAMRGDHTLFTTAEGIERLWEVSIPLLESPPPVR
                     LYEPGSWGPKSIHQLVAPHAWRLPFERAWRKPNTTGA"
     gene            complement(3241..3489)
                     /locus_tag="VGM72_00020"
     CDS             complement(3241..3489)
                     /locus_tag="VGM72_00020"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY4263674.1"
                     /translation="MVEDGEDARAQIKAGDIFTPAVVRKRLAMTQEQFARALRIPVAT
                     LRNWEQGRNALDPAARSLLILVARDPETVLAVLAAEAA"
     gene            3636..4439
                     /locus_tag="VGM72_00025"
     CDS             3636..4439
                     /locus_tag="VGM72_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY4263675.1"
                     /translation="MNHRWDVDDTTIIELKIGGFAKSILTVNGNAVPSSLTIRRKSEF
                     PFVIPDGRHAAISIRPQFAGVPRVELRVEGNLMVPTEKTPVKCPSCDAVVKPNDRFCS
                     VCGKAMPPAENYIFQKSAKEATTAIWTLAVLFAISGVIMFFVTKSQDAAALARLNSLN
                     PNDPFPNSINGVAYTVAALRDKILWEPWGVLIVNLILAVVMMGLAIWGKRAPLAAALV
                     ATATYIVVIVTNAVIAPASIGQGLYLKIIVIIFLVRGIKGALALRSAHA"
     gene            4543..5226
                     /locus_tag="VGM72_00030"
     CDS             4543..5226
                     /locus_tag="VGM72_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEY4263676.1"
                     /translation="MDTPDRPAAAGPNSNFELHWGKIKQIGLLFGLLLASSLFLGIIG
                     RFNDSPWSEAIVSAIDALIVIAFASLHYRQILPLLGLPKMGGRLALRLLAVAIGFAIA
                     MTIYFALLQRLGVPMLRVSDNYRKAGWGLWAMLVLVSLTPAVVEELAFRGVIQSTLES
                     IFDRRDAWIIQAALFSVLHLSPIIFPSHFIMGLCFGYLRLRSKSLYPGIILHGSWNAL
                     VLFQELYWP"
     gene            complement(5328..6518)
                     /gene="carA"
                     /locus_tag="VGM72_00035"
     CDS             complement(5328..6518)
                     /gene="carA"
                     /locus_tag="VGM72_00035"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012053752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEY4263677.1"
                     /translation="MTDGTSTAAPHSGALESPARPTGVLVLADGTVFEGRAFGAPTRA
                     VGEVCFNTAMTGYQEILADPSYAGQIVAFTFPHIGIVGANEEDIESITPAVRGLIVRA
                     HAENPSNYRAAMTLDRWLKRHNIPGLSGIDTRRLTARIRESGMPHGVIAHDPDGRFDL
                     SGLRAQAKDFPGLVGLDLAKEVSCRQMFTWDETPWRWNEGYGRQENPSWRAVVVDYGV
                     KRNILRLLAGAGAEIIVVPASSTAEDVMRHKPHGVLLSNGPGDPAATGQYAIPMIRGL
                     IDAETPLFGICLGHQMLGLALGGQTRKMAQGHHGANHPVKDLETGKVEIVSMNHGFTV
                     DRESLPKYVKETHVSLFDGTNCGIRVEGQSIFSVQYHPEASPGPMDSHYLFDRFATAA
                     KTRT"
     gene            6684..7136
                     /locus_tag="VGM72_00040"
     CDS             6684..7136
                     /locus_tag="VGM72_00040"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEY4263678.1"
                     /translation="MGLRDRFTDAMKLAMKAKEAKRLGTIRLMMAEVQKQGIATKNEN
                     PTDDEILATLAKMIKQREDSVTAYEAGARPELAAVEREEIAVIREFMPRQMSEEDAKA
                     AVTEAIAATGAASLKDMGKVMAALKERHTGAMDFGRASATVKELLAPK"
     gene            7246..>8886
                     /gene="dnaG"
                     /locus_tag="VGM72_00045"
     CDS             7246..>8886
                     /gene="dnaG"
                     /locus_tag="VGM72_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY4263679.1"
                     /translation="MRYGEGLLEEILRRTDLVQLVGRSIKLDRKGRVFWGLCPFHKEK
                     SPSFKVENERRTYHCFGCGAGGDAFKWLTETESLSFPEAVQKLAGEAGIELPQWSAED
                     EARETRKKSLFEIIGLAAQFFEEQLHRMRAGSTARAYLQSRGLEAGAWQRFRLGYAPD
                     GNSSLRDHLIAKGVALADMIEAGLVRPADDNRQPRDFFYDRVMFPIADVRGRIVAFGG
                     RGLSPDAKPKYINTGETPLFSKGHLLYNFKPAREVALKGEPLIVAEGYMDVIALVEAG
                     FAGAVAPLGTALTEDQLALLWKMSPEPILCFDGDEAGLRAARRASLLALPHLEPGQSL
                     RFVFLPQGEDPDSFIRSQGAEPMRAALAKAQGLADMLWAGETEGRDLSTPERRAGLEA
                     ELERIVKTIRDPKIADYYRRDFSERVFKAFRQRQSFSGKSAFSGKSTFAGNPRNGEGR
                     RGNFRPNFGRPGTPPAQDVSAAVKRSLHVVNALSAAKNLNERRLLALLVCSPHLIDRY
                     SEALAWLALDDPQLDRIRRELLNLAASGKSLDKAAVENH"
BASE COUNT         1746 a         2691 c         2686 g         1763 t
ORIGIN      
        1 cccggcccaa tcccgaccac gccagaccgg aaatctcgta atagcccggc ccctgcatgg
       61 cgatgccgaa ggacggcttc aggatcaccg attttggctc catctgcagc atgaactggc
      121 gcgctttgcc gtccggcatc agctcggtgt agcgcgaggt ttcgtccttg gtgtaggtcg
      181 gcgtatccgt caccttcaac cgccgcagcc atttcacatt ggtgttgcct tcgaaacccg
      241 gcagcaacag gcgcatggga aagccctgct cggggcggat gggttcgccg ttctggaaga
      301 gcgcgatcat cgcatcgtcc atcgccttcc acaccggtac gctgcggctc atgccggcgc
      361 catccgcacc ttcggcgaga atccaacttg ccttcggatc gaggcccgct tcgtcgagca
      421 gcgtggaaag cttcacccct gtccattcgg accccgaaag cagcccgtga atatcgcccg
      481 cgctctgctg cggggcctgc ggcgcattca tgccgcccga attgcccgcg cattccatga
      541 aacggatttc ggactgcatc ggatagcgca tcagcgcatc catgccgaac accagcggct
      601 gcttgaccat gccatgaatc gtcagccgat gcttgtcggg atcgatatcg ggcacgccat
      661 tgtggatgcg cacataatgc aggccggccg gcgtgatggc cccttcgaga agttggaggg
      721 gcgtgcggga agacccggtg acttcgcgcc ccggcggcgc attggcgacg aggatgcgct
      781 tgatgttctc gcgccaatgc gagggcatgc cgtacgcggt aaagcccgat ccaggtttca
      841 gcatggacgg cggcgagagg ctgccgatgc tttcggcgcg cgccggattg gcggcggcga
      901 tccctgccag cgcggcaccc gattgcagga aaaaccgccg gtgcagcagg cctccgccgg
      961 caacaggttc gcccaattgt tcggcaagac tgcgcgcgtg ggccatggcg ccctcccctg
     1021 ggattttgcg cttaattcca catcatcatt agcgcgaaac cttgcgcgac tccatgcggg
     1081 cgacaccacg acggcgcaca aaccgttggt atcaaataaa tatcacgata tcgcgtcgag
     1141 gcacgcgagg tcgcggctga ggtcttcgag gaatttgatt tcgcggggcc gcctgacgcc
     1201 atcggcatcg acgacgcgtt gcgcggcggc aacaaggctg acgatgtcgc catgggaatc
     1261 atggtgcaac cgcttcagcg cgggaccgaa ctgggcacgg gatgggcgaa acgcgtgcag
     1321 ataatcgctg agcgccgtct tctccatcaa cgacagctcc aaacccgaaa gtttcgcgcg
     1381 gtccagacaa tgatccagga tgacctcacg ttcctgcacc gcgcattcac cgtcacagcg
     1441 ggcaatcaga aggagcgtga cgaggtgatg cccgaaggcg tcgcggctgc gcatcaaatc
     1501 gctcggaaaa tcctcgacta tccccgcagg ctccggtggc agaggctgtt cacttacgaa
     1561 actggcaaaa cgcccgacaa gaagaccgag actcattttt ccggatgccc accctgataa
     1621 tcaggcgcca aattatgcag tttcggtgac agttggatgt cggtgcgtcg atgacgcagg
     1681 cccaaatcaa tgttctgaaa gggatttcgg ttaagctgag cctcgatagg cgtggtgacg
     1741 ccgctcagac aggatgggtt ccttggcaaa gattcctccg ggggttggac cgaaatatcc
     1801 ccaggtcgtg gttctggtcg gcgccacggg cgatctgtcg cgacgcaagc tgctgcctgg
     1861 gcttttccat ctgtcgcgtg cgggattcat tcccggatgc cgcatcatcg gggtctcgct
     1921 ggacaatatc gacgccgatg gctttcgcgc catcgcgcgc gaggcgctcg atcaattctc
     1981 gacacggaaa atgagcgagt ccgattggtc aaacttcgcg cagagcctgg attatgtgcc
     2041 gctcggcgcc ggtgccaccg gcctcaaagc cgccgtcgac aaagcggaaa gcctgctggg
     2101 ccgcgaatgc cgccggctgc attacttaag cgtgccgccc gccgctgctc tctccgccgt
     2161 gcgccttctg ggcgaggccg atctcgtcga gcgttcccag atcgtcatgg aaaagccgtt
     2221 cggcaccgac ctcgcaagcg cggtgacgct gaatgcccgg ctgcacgagg tgttcgccga
     2281 acatcagatc ttccggatcg atcacttcct gggcaaggaa ccggcacaga acatcctcgc
     2341 cttccgtttc ggcaatggtc tgttcgaacc gatctggaac cgcaatttca tcgatcatgt
     2401 tcagatcgat gtcccggaaa cattggggct gggcaaacgc gcgggcttct acgaacagac
     2461 cggcgcctat cgcgacatgg tggtgacgca tctgttccag attcttggct ttatggcgat
     2521 ggaaccaccg acctcgctcg agcccgcacc gatcagcgag gagaagaaca aagttttccg
     2581 cagcatggtg ccgatcgaac ccaagaatgt cgtgcgcgga caatatatcg gctatcgcgc
     2641 cgaggagggc gtggacccgg aatcggacac cgagactttc gttgcgctga aatgcagaat
     2701 cgacaattgg cgctgggccg gtgtgccgtt tttcctgcgc acgggcaagc ggctcgccga
     2761 aggccagcgc atcatctcca tcgcatttcg cgaaccgccc aagagcatgt tccccgtggg
     2821 ttcgggcgtc ggcgcgcaag gccccgatca tctgaccttc gatcttgcgg acgcgtcaaa
     2881 gatgtcgttg tcgttctacg gcaagcgccc cggtcccggc atgcggctgg acaaactcag
     2941 tctgcaattc gccatgcacg acaccggcct gatcggcgac gtgctggagg cgtatgaacg
     3001 tctgatcctc gatgcgatgc gcggcgatca cacgctgttc acgaccgccg aaggcatcga
     3061 gcggctgtgg gaggtatcga ttcctctgtt ggaatcgccg ccgccggttc ggctgtatga
     3121 acccggttca tggggcccga aatcgatcca tcagctcgtg gcgccccatg cctggcgtct
     3181 gccgttcgag cgcgcctggc gcaagcccaa caccaccggc gcgtgactta aggccaaaac
     3241 ctatgcagcc tccgctgcga gaacggcgag taccgtttcg gggtcgcgtg cgacgagaat
     3301 caacaaggac cgcgctgccg gatcgagtgc attgcggccc tgttcccaat tgcgcaacgt
     3361 cgcgacagga atgcgcaagg ctcgtgcgaa ttgctcctgc gtcatggcga gtcgcttgcg
     3421 caccacggcc ggcgtgaaga tatcaccggc ctttatttgg gcgcgcgcgt cttctccgtc
     3481 ctcgaccatg tgacggcgaa tgtctttctc gcgcccgatc gaccttcgag tgaggttctt
     3541 cgaacacgca ttttaaccca aatgcgagat tcgaaagagc ggcctgaaag atcgtgtgat
     3601 atcttccggg agggcgggtt gtgtttgagg ggaccatgaa tcaccgatgg gacgttgacg
     3661 ataccaccat aatcgaactg aaaatcggcg gcttcgcgaa gagcatcttg acggtgaacg
     3721 gaaacgccgt cccaagctcg ttgacgatcc ggagaaagag tgaatttcct tttgtgattc
     3781 ccgacgggcg tcacgccgcg atttccatta ggccgcaatt cgcgggcgtt ccacgcgtag
     3841 agcttcgcgt ggagggaaat ctcatggtcc cgactgaaaa aacgcctgtg aagtgtccat
     3901 catgcgacgc ggtagtgaag cccaatgacc gtttttgcag cgtttgtggc aaagctatgc
     3961 caccggcaga aaattatatt tttcagaaat ctgccaagga agcgacgacg gcgatctgga
     4021 ctctggctgt tctgtttgcg atctccgggg tcattatgtt cttcgtaacc aagtcgcaag
     4081 atgcggcagc attggctcga ctcaacagct tgaacccgaa tgatccattt ccgaactcga
     4141 taaacggcgt ggcctatacg gtggcagccc tgcgcgacaa gatcctctgg gaaccatggg
     4201 gagttctaat cgtcaacctc attctggcag tggtaatgat gggtcttgcg atctggggca
     4261 aacgtgcacc cttagcggcg gcgctggttg ccactgctac atacattgtc gtcatcgtga
     4321 ccaatgccgt gatcgcccct gcttccattg gtcaagggct ctacttgaaa attatagtca
     4381 tcatttttct cgtaagaggc atcaaggggg cgttggcgct gcgctcggcg cacgcgtgat
     4441 acgaatctga atctcgccca tcagcactgt actaaatgcg agcagtcgtt gcggcccgga
     4501 gcgcaatttt gcggcgcctg cggttacgca gttgcaggtg aattggacac gccagatagg
     4561 cctgcggcag caggcccgaa ctcaaacttt gaattgcatt ggggaaaaat caaacaaatc
     4621 ggtttgctct tcggactgtt actcgcaagt tccctctttc ttggaatcat cgggcgcttc
     4681 aatgattctc catggtccga ggctatagtc agcgcaatcg acgcgttgat agtcattgca
     4741 ttcgccagcc tacattatcg acaaatcttg ccgctgctgg gactgcctaa aatgggcggg
     4801 cgcttggccc ttaggttgtt ggcagtcgcg ataggctttg ccatagcgat gaccatttac
     4861 ttcgctttgc tccagcgact aggggttcca atgttacgcg tttcggacaa ttaccggaaa
     4921 gccggctggg gcttgtgggc aatgctcgtt ctggtcagtt tgacacctgc cgtggtcgaa
     4981 gaattagcgt ttcgtggcgt gatccaatct acactggaaa gcatttttga tcggcgcgat
     5041 gcttggatca tacaagccgc gttgttcagc gtcttacact tgtcgccaat tatttttccc
     5101 agccatttca taatgggctt gtgcttcggc tacctgcgtc tgcggtcgaa gagcctttat
     5161 cctggtataa tattgcacgg atcgtggaac gcgcttgttc tattccaaga gctctattgg
     5221 ccttaacgtc ctcatagcta ttcttggcga gaagcggacg caactacgcc agtcatcgaa
     5281 cgactgcttc taaccatcgg cgcattcgag tgaggaattg aagagcgtta cgtccgcgtc
     5341 ttggcggcgg tggcgaagcg gtcgaacaga tagtgcgaat ccatcggacc gggtgacgcc
     5401 tccgggtggt attgcaccga gaagatggat tgcccctcga ccctgatccc gcaattggtg
     5461 ccgtcgaaca gcgagacatg cgtctctttc acgtatttcg gcaggctctc gcggtccacc
     5521 gtgaagccgt gattcatcga gacgatctcg accttaccgg tctccagatc cttcaccgga
     5581 tggttcgcgc catgatggcc ctgcgccatc ttgcgggtct gtccgccgag tgcgaggccc
     5641 agcatctgat ggccgaggca gatgccgaac agcggcgtct cggcatcgat cagtccacgg
     5701 atcatcggga tggcgtattg tccggtcgcc gccggatcgc cgggaccgtt ggagagcagc
     5761 acgccgtgcg gcttgtggcg catcacatcc tcggcggtgg aggatgcggg caccacaatg
     5821 atctccgcgc cagcgcccgc gagcagcctg aggatgttgc gcttgacgcc gtaatcgacc
     5881 accaccgcgc gccagctcgg gttttcctgg cggccatagc cctcgttcca gcgccacggc
     5941 gtctcatccc aggtgaacat ctgccggcag ctcacctcct tggcgagatc gaggcccacg
     6001 agtcctggaa aatccttggc ttgtgcacgc aaacccgaaa gatcgaagcg accgtcggga
     6061 tcatgggcga tgacgccgtg cggcatgccg ctttcgcgga tgcgcgcggt cagccggcgc
     6121 gtatcgatgc ccgaaagccc cggaatattg tggcgcttca gccagcggtc gagcgtcatg
     6181 gcggcgcgat aattggacgg attctcggca tgggcgcgca cgatcagtcc gcgcaccgcc
     6241 ggcgtgatcg attcgatgtc ttcctcattg gcgcccacga tgccgatatg cgggaaggtg
     6301 aaggcgacga tctgccccgc ataggagggg tcggcgagaa tttcctgata gccggtcatc
     6361 gcggtattga agcagacttc ccccaccgcg cgggtgggcg ccccgaaggc gcggccctcg
     6421 aagaccgtgc cgtccgccag caccagaact cccgtcggac gcgcagggga ctccagcgcc
     6481 ccagagtgtg gagcggcggt gcttgtgcca tcggtcattg gccatacctg tgggaattcg
     6541 tgcgccgcac catagggaga ggcccgaacc gcgtcaagcg cggcttgtgg acaaaatatg
     6601 tagatatttc agatgtttac ggcatttgcc ggggctgacc ctttggccta gtgtgcgggc
     6661 gtcttaacgt caggaaccga ctcatgggtt tgcgcgaccg cttcaccgac gccatgaagc
     6721 tggccatgaa ggccaaggag gccaaacgcc tcggcaccat ccgcttgatg atggcagagg
     6781 tgcagaagca gggcatcgcc accaagaacg agaaccccac ggacgacgaa atcctggcca
     6841 cgctcgccaa gatgatcaag cagcgcgagg attccgtcac ggcctatgaa gcgggcgcac
     6901 gccccgaatt ggccgcggtc gagcgcgagg aaattgccgt catccgcgaa ttcatgcccc
     6961 ggcagatgtc cgaggaagat gcaaaggcgg ccgtgacgga ggcgatcgcg gccacgggcg
     7021 cggcctcgct caaggacatg ggcaaggtga tggccgccct gaaggagcgc cacaccggcg
     7081 ccatggattt cggcagagca agcgccaccg tgaaagagct gctggctccg aaataagtgt
     7141 catcccggcc agcgcagcac gagccgggat ctatcttggt attttcgctg tgggtcgcgg
     7201 atcgagcggc tatcgccgct ccgtccggga tgacacattg caatcatgcg ctacggcgaa
     7261 ggcctcctcg aagaaatctt aaggcggacc gacctcgtcc aactggtggg ccgaagcatc
     7321 aagctcgacc gcaagggacg ggtgttctgg ggcctttgtc ccttccacaa ggagaaatcc
     7381 ccctccttca aggtcgagaa cgagcggcgg acctatcatt gcttcggctg cggcgcgggt
     7441 ggggatgcct tcaaatggct gaccgaaacc gagagcctga gtttccccga ggccgtacag
     7501 aaactcgccg gcgaagcggg gatcgagctg ccgcaatggt ccgccgaaga cgaagcgcgc
     7561 gagacccgca agaaatcgct ctttgagatc atcgggctgg cggcgcaatt cttcgaggag
     7621 caattgcacc gcatgcgtgc cggcagcaca gcgcgcgcct atctgcaatc gcgcgggctc
     7681 gaagcgggcg cctggcagcg ttttcgcctc ggctacgcac ccgacggaaa ttccagcctg
     7741 cgcgatcatc tgatcgccaa aggcgtcgca cttgccgaca tgatcgaagc gggattggtg
     7801 cggccggcag acgacaatcg gcagccacgc gatttctttt atgaccgcgt gatgtttccg
     7861 attgccgatg tgcgcgggcg catcgtggcg ttcggcggcc gtggcctgtc acccgatgcg
     7921 aagcccaaat acatcaacac cggcgagacg ccgctttttt ccaagggcca cctgctctac
     7981 aatttcaagc ctgcgcgcga ggtggccctc aagggcgagc cgctgatcgt ggccgaaggc
     8041 tatatggacg tcatcgcgct cgtcgaggcg ggcttcgccg gcgcggtggc tccgctcggc
     8101 accgcactga ccgaggacca gcttgcgctt ctgtggaaaa tgtcgcccga gcccatcctg
     8161 tgcttcgacg gcgatgaggc ggggcttcgc gccgcccggc gggcgagcct cctggcgctt
     8221 ccccatctgg agcccggcca atccttgcgc ttcgtgttcc tgccgcaggg agaggacccc
     8281 gacagcttca tccggagcca gggcgccgaa ccgatgcgcg cggcactcgc caaggcgcag
     8341 ggcttggccg acatgctgtg ggcgggcgaa accgaaggcc gcgatctttc gacgcccgaa
     8401 cgccgtgccg ggctggaggc ggaactcgag cgtatcgtca agaccatccg cgatccgaaa
     8461 atcgcggatt actaccgccg ggatttctcg gaacgcgtct tcaaggcctt caggcagcgc
     8521 caatcttttt cgggtaagtc ggctttctcc gggaaatcga cttttgccgg caacccgcgc
     8581 aacggtgaag gccgccgcgg caatttccgc ccgaatttcg gccgccccgg cacgccgccc
     8641 gctcaagacg tctccgccgc cgtcaagcgc agcctgcatg tcgtgaacgc cctttcggcg
     8701 gcgaagaacc tgaacgaaag gcggcttctg gccctactcg tctgttcacc ccatttgatc
     8761 gaccgctatt ccgaagcgct ggcttggcta gctttggacg acccccaact tgacaggatc
     8821 agacgcgaac tcttaaacct cgcagcctcg ggcaaaagcc ttgacaaagc ggccgttgag
     8881 aaccac
//
LOCUS       DASYEG010000002         8913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1885027, whole genome shotgun sequence.
ACCESSION   DASYEG010000002 DASYEG010000000
VERSION     DASYEG010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 8913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8913
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1885027"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..648)
                     /gene="hisI"
                     /locus_tag="VGM72_00050"
     CDS             complement(214..648)
                     /gene="hisI"
                     /locus_tag="VGM72_00050"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF000768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEY4263680.1"
                     /translation="MFRRRGRRLHVRQGEAWHVRHPILLGSPRLSEENLTLERFLGAV
                     VFDANGLVPAIAQDAKSGDVLMLAWMNAEALRLTLQTQEVTYWSRSRKSLWRKGETSG
                     HTQALVEAYVDCDGDTLLLKVDQVGPACHTGASACFFRKLNP"
     gene            complement(558..857)
                     /locus_tag="VGM72_00055"
     CDS             complement(558..857)
                     /locus_tag="VGM72_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263681.1"
                     /translation="MAVDGTWKLSVNTPMGAQESTLVIANSGGTPTATQSAGSSDARP
                     VDDLTIHGNEVSWKSSITRPVALTLEFSGAVEGDSMSGKVKLGMFGTQSFSGVRA"
     gene            complement(897..2045)
                     /gene="metC"
                     /locus_tag="VGM72_00060"
     CDS             complement(897..2045)
                     /gene="metC"
                     /locus_tag="VGM72_00060"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEY4263682.1"
                     /translation="MTRKETKIIEAGRDPALHAGAVNTPVYRMSTVLFPDLETMRTEA
                     QPYTYGRRGTPTTRALEQALCGLEGGARTVLTPSGLSACTLAILSLCGAGDHLLVTDS
                     VYGPTRIFCQKLAKRYGIATTYFDPCIGAGIAQLFRPETKAVFLESPGSFTFDVQDVP
                     AIAHIAHERGASVILDNTWATPLGFDALGHGVDLSVQAVTKYISGHADVLMGAITANS
                     AHKDRLVEAHGTLGLCASGDEAYLAMRGLRTLAVRLKRHQESATEIAAWLKTRPEVAR
                     VIYPPLHDDPGHALWKRDFTGGCGLFGVVLKPASDAAVAAMVDGLEHFGLGYSWGGFE
                     SLIVNGHFPRSYPPALEGPLLRLHIGLEDVEDLKADLTAGFERLRRSA"
     gene            complement(2042..3442)
                     /locus_tag="VGM72_00065"
     CDS             complement(2042..3442)
                     /locus_tag="VGM72_00065"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEY4263683.1"
                     /translation="MSGIAAAREQTHIRPRELLGHPAGLAVLFETEMWERFSYYGMRA
                     LLVLYLTKYLLLPGHVENVWGYGAIKGFFESLAGPLDVQPLSSLIYGSYTGLIYFTPL
                     PGGWLADRVMGQKRVVVLGIVMMACGHFMMASERLLFPALFLLIVGGGIFKPNTTGQV
                     GSLYAAGDHRRDRAYSIFYVGTNIGSFLAPLVCGTLGEEAGWHYGFAAAGVGMLFALA
                     IYLIGFSTLPEDELTKERVTHTERPKLTKEDKRAVWALIFLCIPLTLWWACYEQQGNV
                     LNLWTADNTNRGIDLYFWRGEIPVTWFQSVNPFMIFAFTPFLLGLWTRQAAKGTEPPS
                     LNKIMFGCLCQMAGYLVLASVAAYVGDTGKASWLWLPVFIGLVTIGELYLSPISTSLY
                     SKLAPPQILSTMMAVNFIPNFLGGGLLQGWLGAYWSSMPKAEFFLMIAIISGISGIIV
                     WSFHRPLKSILKEEAA"
     gene            complement(3439..5415)
                     /locus_tag="VGM72_00070"
     CDS             complement(3439..5415)
                     /locus_tag="VGM72_00070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEY4263684.1"
                     /translation="MSTSESSQRHGAELTLRALILGALITLVFTAANVYLGLKVGLTF
                     ASSIPAAVISMAVLSVFPGSNIRENNIVQTVASAAGALASIIFVLPGLVIVGWWTGFP
                     FWESFLVCASGGTLGVLFTIPLRRALVTNSDLPYPEGVAAAEVLKVGSGLHESGANAG
                     TAREGLFAILLGSLASAGLAIFAATRIAAGELQSFFRLGTNSATGYDLAFSLALMGAG
                     HLVGISVGLAMLTGLLIAWAGALPILTALQPDSGAALETQVMHVWSTQVRFIGAGAMA
                     VAALWTLARLAHPLVSGFVRTIAASRAAAISSGDQSDRDLSGHTIIGLTVACLIVITA
                     LLWNFARESVLAGAALPLTLSALPFVVIAGFIVAAICGYMAGLIGSSNSPVSGVGILA
                     IVTCATIFLLVAPPTQETAPALVAFALFATAIVFGVAVTSNDNLQDLKTGQLVGAAPW
                     RQQIALIVGIIAGALVIPSILNLLAQAYGFAGAANVAAVTAKPLPAPQASLISTLALG
                     VIGHKLDWNMIGIGAGIGAIIVAIDSFLGQRKWLRLPPLAVGMGIYLPMSATLPVMIG
                     SLIGWWYNRRVAKGPDPARAQHLGVLVASGMIVGESLFGVLLAALIVGLNSDAPLALV
                     AGDFAPADAVGTLAFIALIVLLYGWMLRRARLAQ"
     gene            complement(5420..5974)
                     /locus_tag="VGM72_00075"
     CDS             complement(5420..5974)
                     /locus_tag="VGM72_00075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4263685.1"
                     /translation="MTRGKDTRPRELTAIVTYLGMEAKPKTASPPAPLLKTALLKCDD
                     PPVHFYRYLYDTIGRRYFWVERRLWNDQKIKAHLTDESLALYALYLGGVPAGMAELDF
                     REPGIGQIAYFGLIPEFTGRHVGPWFLHQVIEICWAAPITRLLVNTCTLDHKKALVTY
                     QRAGFVVYARTERAVLVPPDFPAS"
     gene            complement(5971..6828)
                     /gene="sseA"
                     /locus_tag="VGM72_00080"
     CDS             complement(5971..6828)
                     /gene="sseA"
                     /locus_tag="VGM72_00080"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HEY4263686.1"
                     /translation="MSKAIPPLVSTEWLAANMWQPHVVIADASWYLPQAGRDAHAEYK
                     AAHIPGALFFDIDAISDQNSTLPHMLPPAVAFASSMRKLGIGDSDIVVFYDGAGIYSS
                     PRAFWMMRVMGHRNVAVLDGGLPKWRREGRPVEAVPAAPVARGHFTPRRQDHLIRDFA
                     AMRSNLQTRDEQIVDARSPSRFRGEEAAPRAGVRPGHIPGSINLHYAEVLTLEGTMRN
                     VDDLQRLFAERGIDLARPVATSCGSGITAAILALAFEIAGAKSVTLYDGSWSEWGAAH
                     DAPVETGRR"
     gene            complement(6821..7864)
                     /locus_tag="VGM72_00085"
     CDS             complement(6821..7864)
                     /locus_tag="VGM72_00085"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF007989.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEY4263687.1"
                     /translation="MPQTQIKKDVLAAIGDTPLIRLKHASEITGCEILGKAEFMNPGQ
                     SVKDRAALFIIRDAMTKGQLKPGGTVVEGTAGNTGIGIAVVGNALGLKSVIVIPDTQS
                     QEKKDALRLLGATLIEVPAVPYKDPNNYVKISERLAAQLAKSEPNGAVWANQFDNVAN
                     REAHIQTTGPEIWQQTGGTVDGFVCSAGTGGTVAGVAIALKQRSKKIVTAVADPLGAA
                     IYSWIKTGELKGEGSSITEGIGQGRVTGNLAGAPIDDAFLIPDEEALPLIFELLQDEG
                     LCLGGSSGINVAGAIRLARQLGPGHTIVTILADYGTRYQSKLFNPEFLRSKNLPVPEW
                     LAAPAPLPGVPYE"
     gene            7863..8324
                     /locus_tag="VGM72_00090"
     CDS             7863..8324
                     /locus_tag="VGM72_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263688.1"
                     /translation="MKLLWKRPMVRNEPKETGRDLQERKSPGGTGSRFHVKRYPFQVR
                     ESRVPVRIVQTALSLVPGGVHFSNSAAKSAYPAMTYLTVQRLYFRWQFFGIAIFGALL
                     LNGTAAAMLAGQAGFSWALAACLLLIVSLGIFFIWTYPVKQATASLALAKR"
     gene            8407..>8913
                     /locus_tag="VGM72_00095"
     CDS             8407..>8913
                     /locus_tag="VGM72_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263689.1"
                     /translation="MKNLLFAGSCAACLALAAAVFAPAGAQQQTVLAIQGGTLIDGNG
                     GAPVPNSVIVIQANRITAVGRAGQVQIPAGATLINAAGKWVLPGLIDAKSNWNWPYGE
                     GYLRYGVTSAMVTGTRNDTGIADRDAVEHGVYPAPRLYQGVINLQGGGAEGKKPDNYK
                     PGNGARIVK"
BASE COUNT         1825 a         2724 c         2751 g         1613 t
ORIGIN      
        1 ttggccagca ccggctgctg ataagaccag atgcgcgcgc tgcccgtcgc ggggtgcagc
       61 agcacgaccg cttcaccatt gccgcctgta tcccaatagg cgaggcgcgt gccggggagg
      121 gacgcaagag cttccttcgc cggaacctgc ggtggaatcg gaatcgcatc cataggttca
      181 gcgttcacag ggcgcgaggg ggcctgactc cggtcagggg ttcagcttgc ggaagaaaca
      241 ggcggacgcg ccggtgtggc aggccggccc gacctggtcc actttcagaa gcagcgtatc
      301 accgtcgcaa tcgacatagg cttccacgag agcttgcgtg tggccgctgg tttcaccctt
      361 gcgccacagc gatttccgcg accgcgacca ataggtgact tcctgcgttt gcagcgtcag
      421 gcgcagggct tccgcgttca tccaagcgag catcaggacg tcaccggatt tcgcgtcctg
      481 ggcgatggcc gggacaagcc cgttcgcatc gaacaccacg gcgccgagaa atctttctaa
      541 tgtgagattt tcctcgctca ggcgcggact cccgagaagg attgggtgcc gaacatgcca
      601 agcttcacct tgccggacat ggagtcgcct tccacggcgc cggaaaattc cagagtcagc
      661 gcgaccggcc gcgttatcga ggatttccag ctcacctcat tgccatggat cgtcagatca
      721 tccaccgggc gggcatcgct cgatccagca ctctgtgtcg cggttggcgt gccgccggaa
      781 ttcgcgatca caagtgtgct ttcctgcgcg cccataggcg tattgaccga taatttccaa
      841 gtgccgtcga ccgccatgcg gttctccttt tcgtttttcc attaatccat agcacgctac
      901 gcgctccggc gcagacgttc gaaacccgcg gtcagatccg ccttcaaatc ctcgacgtct
      961 tcgaggccga tatgaagccg cagcaacggt ccttcaagtg cgggcggata ggatcgcgga
     1021 aaatggccgt tcacgatcag gctttcgaac ccgccccagg aataacccag accgaaatgt
     1081 tcgagcccat ccaccatagc ggcaacagcc gcatcggagg cgggcttcag caccacaccg
     1141 aagagcccgc agcccccggt gaagtcgcgc ttccagagcg cgtggccggg atcgtcatgc
     1201 aggggcggat agatcacgcg cgccacttcg gggcgcgtct tcagccaggc ggcgatttcg
     1261 gttgcgcttt cctggtggcg cttcagcctg acggccagcg tgcgcagccc gcgcatggcg
     1321 agataggctt catcgcccga tgcgcaaagg cccaacgtgc cgtgggcttc caccagccgg
     1381 tccttatggg cgctgtttgc ggtgatcgcg cccatcagga cgtcggcgtg accgctgata
     1441 tatttcgtca ccgcctgaac ggagaggtcg acgccatggc cgagcgcatc gaagccgagc
     1501 ggtgtcgccc atgtattgtc gaggatcacg ctcgcgccgc gctcatgggc gatatgcgcg
     1561 atggccggca catcctgcac gtcgaatgtg aacgagccgg ggctttccag aaacaccgcc
     1621 ttggtttcgg ggcggaatag ttgcgcgatg ccagcgccga tgcaggggtc gaaataggtc
     1681 gtggcgatgc cgtagcgctt tgcaagcttc tgacagaaga ttcgtgtcgg cccatagacg
     1741 gaatcggtga ccaatagatg gtcgcccgcg ccgcacaggg aaaggatggc aagcgtgcag
     1801 gcggaaagtc ccgagggcgt gagcaccgtg cgcgcgccac cttccaagcc gcaaagtgcc
     1861 tgttccagcg cgcgcgtcgt gggcgtgccg cgccgcccgt aagtataggg ctgggcttcg
     1921 gtgcgcatgg tttcgagatc gggaaacagc accgtcgaca tgcgatagac gggcgtgttc
     1981 accgcgcccg catgcagtgc gggatcgcgt ccggcctcga tgatcttggt ttccttgcgg
     2041 gtcacgccgc ctcttccttc aaaatggatt tcaatgggcg atggaacgac cagacgatga
     2101 tacccgaaat gccggagatg atggcgatca tcaggaagaa ctccgctttg ggcatgctgc
     2161 tccaataggc gccaagccac ccttgtagca atccgccgcc gagaaaattc gggatgaaat
     2221 tcaccgccat catggtggac aagatttgcg gtggcgccag cttggagtaa agcgaggtac
     2281 tgatgggcga cagataaagc tcgccgatgg tgaccagtcc gataaaaacg ggtagccata
     2341 gccagctcgc cttgccggtg tcgcccacat aagccgccac cgacgccagt acgagatagc
     2401 cggccatctg gcacaggcag ccgaacatga tcttgttgag cgaagggggc tcggtcccct
     2461 tcgcggcttg gcgcgtccat agcccaagga ggaaaggggt gaaggcgaag atcatgaagg
     2521 gattgaccga ctgaaaccac gtcaccggaa tctcgccgcg ccagaaatag aggtcgattc
     2581 cgcgattcgt gttgtcggcg gtccagagat tgagcacgtt gccctgctgc tcgtagcagg
     2641 cccaccacag cgtaagtggg atgcagagga aaatgagcgc ccagacggcg cgcttgtcct
     2701 ctttcgtcag cttcggtctt tcggtgtgcg tcacccgctc tttggtaagc tcgtcctccg
     2761 gcagcgtcga aaagcctatc agatagatgg cgagcgcaaa cagcatgccc acgcccgccg
     2821 ccgcgaatcc gtaatgccag ccggcttctt cacccaaagt gccgcagacc agcggggcca
     2881 ggaacgagcc gatattcgtg ccgacataga aaatcgaata ggcgcggtcg cggcgatgat
     2941 ctccggccgc atagagcgag ccgacttggc ccgtcgtgtt cggcttgaaa atgcccccgc
     3001 cgacgatcag gagaaacaac gcggggaaaa gcaatctctc ggaggccatc atgaaatgcc
     3061 cgcacgccat catcacgatg ccgagcacga cgacgcgctt ttgtcccatc acgcggtcgg
     3121 caagccagcc gcccggcagc ggcgtgaaat agatgagacc ggtataagag ccatagatca
     3181 gcgaggacaa tggctgcaca tcgagcggac cggcgagaga ttcgaagaaa cccttgatcg
     3241 cgccataacc ccagacattc tcgacatgcc ctggcagaag cagatatttg gtcagataca
     3301 gcaccaggag cgcgcgcatt ccgtaatagg aaaagcgctc ccacatttcc gtctcgaaga
     3361 gaacggcgag ccctgccggg tggccgagca attcgcgcgg ccggatatgg gtttgttcgc
     3421 gcgccgcggc gatgccgctc attgcgcaag cctggcgcgg cgcagcatcc agccatagag
     3481 aagcacgatc agcgcgatga acgcgagcgt gcccacggca tcggccggcg caaaatctcc
     3541 ggccacgagg gccagcggtg catcgctgtt cagccccacg atcagtgccg ccagcagcac
     3601 accgaacaga ctctcgccca cgatcatgcc cgacgccacc agcacaccga gatgctgcgc
     3661 gcgcgccggg tccggtccct tggcgacgcg tctgttatac caccagccga tgaggcttcc
     3721 gatcatcacc ggaagcgtcg ccgacatggg aagataaatc cccatgccga ccgcgagcgg
     3781 cggcagacgc agccatttgc gctgaccgag aaacgagtcg attgcgacaa tgatcgcgcc
     3841 gatacccgca ccgatcccga tcatgttcca atcgagcttg tgtccgatca cgcccaaggc
     3901 aagcgtggaa atcaggctcg cctgcggcgc cgggagcggt ttggccgtca ccgccgccac
     3961 attggccgcg ccggcaaagc cataggcttg cgcgagcaga ttcaggatcg aggggatcac
     4021 cagcgcgccg gcgatgatgc cgacgatcag ggcgatctgc tgccgccaag gcgccgcacc
     4081 gaccaattgt ccggttttca aatcctggag attgtcgttc gatgtaaccg cgaccccgaa
     4141 gacaatcgcc gtcgcaaaga gcgcgaaagc aacgagggca ggcgcggttt cttgtgtcgg
     4201 tggagcgacc agcagaaata tcgtggcgca ggtgacgatt gcgaggatgc ccacaccgga
     4261 tacaggacta ttggaggagc cgatgagccc cgccatgtag ccgcagatcg cggcaacgat
     4321 gaaacccgcg atcaccacga agggaagcgc ggaaagggtc agcggcagcg ccgcgccggc
     4381 aagaaccgat tccctcgcga aattccacag caaggccgtg atgacaatca gacacgcgac
     4441 ggtgagaccg atgatggtgt gccccgaaag atcgcggtcg ctctgatcgc cggacgaaat
     4501 cgccgccgcg cgcgacgcgg cgatggtgcg cacgaacccg gagaccagcg gatgcgccag
     4561 gcgcgccagt gtccacaacg cggccaccgc catggcgccc gcaccgatga accgtacttg
     4621 cgtactccag acatgcatta cttgggtttc cagtgcagca ccggaatcgg gctgcagcgc
     4681 ggtcagaatc ggcaaagccc cggcccaggc gatcaggagc ccggtcagca tcgccaggcc
     4741 cactgaaatc ccgaccagat ggcccgcgcc catcagcgca agcgaaaatg ccaaatcata
     4801 tcccgtcgcg ctgttcgtcc cgagacggaa gaaactttgc agttcgcctg ccgcgatgcg
     4861 ggtcgccgca aatatcgcaa gaccggccga tgcaagggag ccgagcagaa tcgcgaacag
     4921 cccctcccgc gccgtgccgg cgttcgcgcc gctttcgtgc agaccggaac cgactttcag
     4981 gacttcggcg gcggcgacac cttccgggta cggaagatcc gaattggtga cgagcgcgcg
     5041 ccgcaaggga atcgtgaaca gcacgccgag cgtgccgccc gacgcacaca ccagaaaaga
     5101 ttcccagaac ggaaatccgg tccaccaacc gacaatgaca agtcccggca acacgaagat
     5161 gatggaagcc agcgcgccgg cggccgatgc caccgtctgc acgatattgt tctcgcggat
     5221 gttcgatccg ggaaagacgc tcagcaccgc catggagatc accgccgcgg gaatcgacga
     5281 tgcaaaggtc aggcccactt tcaggccgag atagacattc gcggccgtga agacgagggt
     5341 gatgagcgcg cccagaatca gcgcgcgcaa tgtcagctcg gcgccgtggc gttggctcga
     5401 ctcgcttgtg ctcatgcgtt cagcttgccg gaaaatccgg cggcaccaga accgcccgtt
     5461 cggtacgtgc gtaaacgacg aaccccgcgc gctggtaggt cacgagagcc ttcttgtgat
     5521 cgagggtgca ggtgttgacc aggagacgcg tgatcggtgc cgcccagcag atctcgatca
     5581 cctgatgcag gaaccagggc ccgacgtggc gtccggtgaa ttccggaatc aatccgaaat
     5641 aagcgatttg accgatgccg ggttcacgaa aatcaagctc cgccataccg gccggaacgc
     5701 cgccaagata gagcgcataa agcgccagcg attcatcggt gaggtgcgct ttgatctttt
     5761 gatcgttcca cagccgccgc tcgacccaga aatagcgccg cccgatggtg tcatacagat
     5821 agcggtagaa atgcaccggc ggatcgtcgc atttgagcag cgctgttttc agcaggggcg
     5881 ccggcgggga tgccgtcttc ggcttcgcct ccattcccag ataggtgacg atggcggtga
     5941 gttcacgggg acgggtgtct ttgccgcgcg tcatcgtctg cccgtttcca ccggggcatc
     6001 gtgcgcggca ccccattccg accaggaacc gtcataaagc gtcacggatt ttgctccggc
     6061 aatctcgaaa gcgagagcaa ggatcgctgc tgttattccc gaaccgcaac tcgttgccac
     6121 cggccgggcc aagtctatgc cgcgttccgc gaacaggcgt tgcagatcgt ccacattgcg
     6181 catcgtccct tccaaggtca gcacctccgc gtaatggaga ttgatcgatc cgggtatgtg
     6241 tccaggccgc acaccggcgc gcggcgccgc ttcctcgccg cgaaaccggg agggacttct
     6301 tgcgtccaca atttgctcgt ctcgggtttg cagattgctt cgcatcgccg cgaaatcgcg
     6361 gatcaaatgg tcctgccggc gcggcgtgaa atggccgcgt gcgacaggcg ccgcagggac
     6421 cgcttcgacg ggacggcctt cgcggcgcca tttgggcagt ccgccatcga ggacggccac
     6481 gttgcgatgg cccatgacgc gcatcatcca aaatgcgcgc ggcgacgaat agatgccggc
     6541 gccatcgtaa aaaaccacga tgtcggaatc gccgatgccg agttttctca tgctgcttgc
     6601 aaacgcgact gccggcggca gcatgtgcgg gagcgtggaa ttttgatccg agatcgcgtc
     6661 gatatcgaag aacaatgccc caggaatatg cgccgctttg tattcggcgt gggcatcgcg
     6721 cccagcctgc ggcagatacc aggatgcgtc tgcaatcacc acatggggct gccacatatt
     6781 ggcagcgagc cattctgtcg agacgagggg cggaattgcc ttactcataa ggcacgccgg
     6841 gcaaaggcgc gggcgccgcc agccactccg ggacaggaag gtttttggag cgcaggaatt
     6901 ccggattgaa cagcttgctc tgatagcgcg tgccgtaatc agcgaggatc gtgacgatgg
     6961 tgtgtccggg tccaagctgc cgcgcgagcc ggatcgctcc ggcgacattg atgccgcttg
     7021 agccgccaag acacagcccc tcatcctgca gcaattcgaa gatcagaggc agcgcctctt
     7081 cgtcggggat caggaaggca tcgtcaatcg gggctccggc gagattgccg gtgacgcgtc
     7141 cctggccgat gccttcggtg atggagctgc cttcgccttt gagttcgccg gtcttgatcc
     7201 aggaatagat cgcggcaccc aagggatcgg caaccgccgt cacgatcttt ttgctgcgct
     7261 gcttcagcgc gatggcgacg cccgcaaccg tgcctcccgt accggcggaa cagacgaacc
     7321 catcgaccgt gccgccggtc tgctgccaga tttccgggcc ggtggtttgg atatgggcct
     7381 cacgattggc cacattgtcg aattgattgg cccagaccgc accattgggt tcgctcttgg
     7441 ccaattgcgc ggcaaggcgt tcggagatct tcacgtaatt gttgggatcc ttatagggca
     7501 cggcaggcac ttcgatgagc gtggcaccca gcagccgcag cgcgtccttt ttttcctggc
     7561 tctgcgtgtc ggggataacg atcaccgatt tcaaacccag cgcattgccg acaacggcga
     7621 tcccgatgcc ggtattgccg gcagtgcctt cgacgaccgt tccaccgggt ttaagctggc
     7681 ccttcgtcat cgcgtcccgg atgatgaaaa gcgcggcccg gtccttcacc gattggccgg
     7741 gattcatgaa ctcggccttg cccagtatct cgcaacccgt gatttcggat gcatgtttga
     7801 ggcgaatgag gggcgtgtcg ccgattgccg cgaggacatc ttttttgatt tgggtttggg
     7861 gcatgaagct tctttggaag agaccgatgg tgagaaacga acctaaagag accggacgtg
     7921 accttcaaga acgcaagtct ccgggcggaa ccggatcccg ctttcacgtg aagcgatatc
     7981 cgttccaggt gcgagaatcg agggttcccg tgcgtatcgt ccaaacggcg ttgtccctgg
     8041 tccccggcgg cgtacatttt tcgaattccg cggcaaaatc ggcctatccg gcgatgactt
     8101 atctcacagt ccagaggctc tattttaggt ggcagttttt tggaatcgcg atttttggtg
     8161 ctttgctgct caatggtacg gcggcagcca tgctggccgg acaggctggt ttttcctggg
     8221 cactcgcagc atgtctcctg ctgatcgtct ccctgggaat attcttcatc tggacttacc
     8281 ctgtgaaaca ggccaccgca tcactggctc tcgccaagag atagacttgg tccaccacag
     8341 gtgctggaac ggttcgggag gctggcctac agtgccctca aaattggtta ttccggggag
     8401 ggtgctatga aaaatctgct gttcgcgggt tcgtgtgccg catgtttggc cctggcggcg
     8461 gcggtgttcg ctccggctgg ggcgcaacag caaaccgtgc ttgctatcca gggtggcacc
     8521 ctgatcgatg gcaatggcgg cgcgccagtg cccaattcgg ttatcgtgat tcaggcaaac
     8581 cgcattaccg ctgtgggccg cgcggggcag gtgcagatac cggccggtgc aaccctcatc
     8641 aatgccgccg gcaaatgggt gctgcctgga ctgatcgatg ccaaatccaa ttggaactgg
     8701 ccgtatggcg agggctatct gcgctatggc gtgacctcgg cgatggtgac gggcacgcgc
     8761 aacgataccg ggatcgccga ccgcgacgcg gtcgagcacg gcgtctatcc cgctccgcgt
     8821 ctctaccagg gggtcatcaa tcttcaaggc gggggagccg aaggaaagaa accggacaat
     8881 tacaaaccgg gaaacggcgc gcggatcgtc aag
//
LOCUS       DASYEG010000003         3085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3190099, whole genome shotgun sequence.
ACCESSION   DASYEG010000003 DASYEG010000000
VERSION     DASYEG010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3190099"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /locus_tag="VGM72_00100"
     CDS             complement(<1..1383)
                     /locus_tag="VGM72_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263690.1"
                     /translation="MGAPVLAVEKLKIESNPFVPGAGGGRMGRPLQTTFGEVVRQIGL
                     ETPVVVVSGSAGTGKTLLANMVTRACAEMGLTIRRIHRGDLVDGAAGERSDLLLIDEA
                     DSMTDLALKTLLLGSAKRPATTTVLLCLPSSVRRFSSAVDTVIVELTRLSQSDTRLYL
                     VERATHAGLTDLFAPAALDLIVDASRGLPRSLWSIAGLAYFSAASAGAVQISRQHVAD
                     ALASQITRPEPAEPPVPAAPSVKLFPEAAIKAPVAAPVAEPPRENANVAYLAAPSSTP
                     PLTNRQPIAEVPVPVVALAPHSETSAAVALPVPVPGLAGVDSKEPQRERAAEAADAPY
                     FLPAFDAALPSDGKQAADAPPVSPEIQEIAAGPIASFDSASAAEGEKPVSPLDAPSLN
                     LLRDTAANDARKGHRSVVVLGVAASLAAVVVIPAMLLFGKFPGTTNAASAPVATLSIP
                     TQAIPRQASVT"
     gene            complement(1653..2558)
                     /locus_tag="VGM72_00105"
     CDS             complement(1653..2558)
                     /locus_tag="VGM72_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263691.1"
                     /translation="MKKIPVGPTIAFAYRFLLTEIGTIVGIAWLPAVLSSAVGYFARL
                     YALENRALLDAGDPQAAGIYLLLSLLSLAVLLFASAMVAVAVTRVAMGQERPPGALLL
                     YFAAGPVEWRMLGANVRYMFGAGALLALAFLISVAAFFLSGAPLDAPEQLRPTPATIL
                     AGVISWAVLIYAGATILRIGFLLPGAVVAEDKGGLRRSHELTKGNFWRILAIILALGL
                     PILFLLLAGEVVVLHAALGPDFMRLSPSAFFQKAGEAMDEKLLPWQIFSAVVFVLASG
                     LIYSGAAFAYRAASQPPNTRPSSVA"
     gene            2667..>3085
                     /locus_tag="VGM72_00110"
     CDS             2667..>3085
                     /locus_tag="VGM72_00110"
                     /inference="COORDINATES: protein motif:HMM:NF013314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4263692.1"
                     /translation="MKQIHIVGGGLAGSEAAWQAAQTGVCVVLHEMRPLRMTEAHATS
                     GLAELVCSNSFRSDDWENNAVGLLHEEMRRAGSLIMGAADSHKLPAGGALAVDRDGFS
                     AAVQAALEAHPNVTLARGEVAGPPADWDNVIVATGPLT"
BASE COUNT          637 a          989 c          980 g          479 t
ORIGIN      
        1 cgtgacgctc gcctgcctcg gaatggcctg cgtcggaatc gacaacgtcg caaccggcgc
       61 gctggccgca ttggttgtgc cggggaattt gccgaacagc aacatcgcgg ggatcacaac
      121 cacggcggcg agggaagcgg ccacccccag aacgaccacc gatctgtgcc ctttgcgggc
      181 atcgttcgcg gcagtgtcgc gaagaagatt caatgacgga gcatccaagg gggagacggg
      241 tttttcaccc tcagccgcgg aggctgaatc gaaagacgcg atgggcccgg cggcaatctc
      301 ttgaatctcg gggcttacgg gcggcgcatc ggcagcctgc ttcccgtcgc ttggcaatgc
      361 ggcgtcgaac gccggaagga aatacggagc gtcggcagcc tccgcagccc gctcccgttg
      421 cggctctttg ctgtccaccc cggcaagccc cggcacgggc acaggcaatg caacagcggc
      481 ggatgtttcg gaatgcggcg ccagcgccac caccggcacc ggcacttccg cgatgggctg
      541 ccgattggtc aacggtggcg tgctggaggg ggcagccaag tacgcgacat tggcattttc
      601 gcgcggcggt tccgcaacgg gggcggccac gggggctttg atcgcggctt cgggaaacaa
      661 tttgacggac ggtgcggcag gaacgggcgg ctctgccggt tcggggcgcg tgatctgcga
      721 ggccagcgca tcggcgacat gttgccggct gatttgcacc gcccccgcgg acgccgcgga
      781 gaaataggca agcccggcga tcgaccagag cgagcgcggc agcccgcgcg atgcatcgac
      841 gatcaggtcc aacgccgcgg gcgcgaaaag atcggtcaat cccgcatgcg tcgcccgctc
      901 cacgagatag agtcgcgtat cggactgaga caagcgcgtc aattcgacga tgaccgtatc
      961 caccgcggaa ctgaagcggc gcaccgaaga cggaaggcac aacaagactg tcgttgtcgc
     1021 gggacgtttg gcgcttccca gaagcagcgt cttcagtgcc aaatccgtca tcgaatcggc
     1081 ttcgtcgatc agcagcagat cggatctctc gccggccgca ccatccacca gatcgccgcg
     1141 atggatacgg cggatggtga ggcccatctc cgcacacgca cgcgtcacca tattcgccag
     1201 caaggttttt cccgtgcccg cacttcccga cacgacgacg accggagtct cgaggccgat
     1261 ttgccggacg acttcgccga acgtggtctg caacggacgg cccatgcgtc cgccgcccgc
     1321 gccaggcacg aacggatttg attcaatttt tagcttttcg acagccagga ctggcgcgcc
     1381 caccgaagcc tcccccaaac tcccgcggtt aactatgatc cggaatggca tgatgcatca
     1441 agtagcgttg cgccgtgcct ggccagaatg cgttggatgc cctccgggtc aagctccaaa
     1501 cttacgcttt cgcggccctg caggaacaca tccaccggca tctgccggga atgattggcc
     1561 gattcagaga tttaccaagg catgcgaaaa aggcgaagcc attcgcaatt actattactg
     1621 ccacctggcg ctggccacgt tcgcgcaacg cttcatgcaa ccgatgacgg ccgcgtgttg
     1681 gggggctggg aagctgcgcg ataggcaaac gcggcaccgc tgtagatcag gcccgacgcg
     1741 agaacgaaaa ccaccgcgct gaagatctgc cacggcaaaa gtttctcatc catcgcctcg
     1801 cccgccttct gaaaaaatgc cgatggactg aggcgcatga agtccggtcc gagcgcggca
     1861 tgcaggacca ccacctcgcc tgccagcagc aaaaacagga tcggcaatcc gagcgccagg
     1921 atgatcgcga gaatgcgcca gaaattcccc ttcgtcagtt catggctgcg gcgcaaaccg
     1981 cccttgtctt cggcaacgac cgcgccggga agcaagaatc caattctcag gatcgtcgca
     2041 cccgcataga tcaagacggc ccaggaaatc actccggcga gaatggtcgc aggcgttggc
     2101 cgtagctgct cgggcgcatc cagcggtgcg ccggacagga agaacgccgc caccgaaata
     2161 aggaacgcca acgcgagcag cgcgccggcg ccgaacatat aacggacatt ggcgccgagc
     2221 atgcgccact cgacagggcc ggccgcgaaa tagagcagta gcgcaccggg cggccgctcc
     2281 tgtcccatcg caacacgggt gacggcgacg gccaccatgg ccgatgcgaa gagcagcacg
     2341 gccaggctca gcagcgaaag cagaagatag ataccggccg cctgtggatc gcccgcatcc
     2401 agcagcgcgc gattctccag cgcatagaga cgcgcgaaat agcccaccgc cgaggacagc
     2461 acagccggaa gccaggcgat gccgacaatg gtgccgatct cggtgaggag gaaacgatag
     2521 gcgaaagcga tcgttggacc gaccggtatt tttttcatca gggttcctgg acgcgaaagc
     2581 gcgcgatgct agggggatga ggggacgctg acaattgcgg ggcggcctat ttgcgcgtat
     2641 ctccccccac tatggtgaaa acgggcatga agcaaattca catcgtcggc gggggccttg
     2701 cgggcagcga ggccgcctgg caggcggccc aaaccggcgt ttgcgtggtc ttgcacgaaa
     2761 tgcgcccgct gcgcatgacc gaagcccatg cgaccagcgg ccttgccgag cttgtctgct
     2821 ccaattcatt ccgctccgac gattgggaaa acaacgcggt cggcctcctg catgaggaga
     2881 tgcggcgggc gggctcgctc atcatgggtg cggccgattc gcacaagctg cccgccggag
     2941 gcgcattggc cgtggaccgc gacggattct ccgctgcggt gcaggcggcg ctcgaagccc
     3001 accccaatgt cacgctcgcg cgcggagagg tcgccggccc tcccgccgat tgggacaatg
     3061 tcatcgtcgc caccggcccg ctgac
//
LOCUS       DASYEG010000004        11951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4784288, whole genome shotgun sequence.
ACCESSION   DASYEG010000004 DASYEG010000000
VERSION     DASYEG010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 11951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11951
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4784288"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1499)
                     /gene="fusA"
                     /locus_tag="VGM72_00115"
     CDS             complement(<1..1499)
                     /gene="fusA"
                     /locus_tag="VGM72_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY4263693.1"
                     /translation="MPRQTPLDHYRNIGIAAHIDAGKTTTTERILYYTGRSHKIGEVH
                     DGAATMDFMEQEQERGITITSAATTCFWNGHRINIIDTPGHVDFTIEVERSMRVLDGA
                     VAVFDAVAGVEPQSETVWRQADKYRVPRICFVNKMDRTGADFYRCVQMMIDRLGTHPM
                     VIQIPVGAEQGFKGVIDLIAMKALIWKEENLGADYEEMEIPEDLREKAQELHTKLVES
                     AVEEDEKLLEAYLEGNMPSVDQLKACIRKGTCAFRFVPVLCGAAFKNKGVQPLLDGVV
                     DFLPSPLDVPPVEGMKPHSEEKIVLQPDDKAPFAGLAFKIMDDPFVGSLTFVRVYSGT
                     MNSGTMVLNSIKDNRERVGRMLLMHANNREDVKEAYAGDIVALAGLKGTTTGETLCDP
                     QHPVILERMEFPDPVIEVAIEPKTKADQEKLGVALNRLAQEDPSFRVSTDLDSGQTIL
                     KGMGELHLEIKVDILRRTYKVDATVGAPQVAYRETLSKSVTVDYTHKKQT"
     gene            complement(1517..1987)
                     /gene="rpsG"
                     /locus_tag="VGM72_00120"
     CDS             complement(1517..1987)
                     /gene="rpsG"
                     /locus_tag="VGM72_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEY4263694.1"
                     /translation="MSRRHRAEKREVIPDAKFGDVVLAKFMNSLMYEGKKSVAEGIIY
                     GAFDTIQQKTKQEPLGVFHEALRNVSPALEVRSRRVGGATYQVPVEVRTDRSRALAIR
                     WIITAARGRNEPTMQGRLSGEIMDAANNRGTAVKKREDTHKMAEANRAFSHYRW"
     gene            complement(2070..2441)
                     /gene="rpsL"
                     /locus_tag="VGM72_00125"
     CDS             complement(2070..2441)
                     /gene="rpsL"
                     /locus_tag="VGM72_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEY4263695.1"
                     /translation="MPTVNQLIRKPRGEKPKRNKVPALTNCPQKRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGYEVVSYIPGEGHNLQEHSVVLIRGGRVKDLPGVRYHILRGVL
                     DTQGVKDRKQRRSVYGAKRPK"
     gene            2928..3050
                     /locus_tag="VGM72_00130"
                     /pseudo
     CDS             2928..3050
                     /locus_tag="VGM72_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000993416.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
     gene            3165..5687
                     /locus_tag="VGM72_00135"
     CDS             3165..5687
                     /locus_tag="VGM72_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4263696.1"
                     /translation="MASRSRKSGGIRKSGFRKRTQHLRAPGALAAALGGVLLMPAYAL
                     AQAPNSNTADSNTAGAIELGPLRVQDQNANALGNDTGLSVLPTTVQDTPQAIIVIPQQ
                     QLREQGVTSLEQALRNVPGITVSIGEGGTLNGDQFKIRGFDAQNDVYVDGLRDFGVYT
                     RDSFAFQEVQVLKGPSGTMFGRGSTGGVINTVSKTPFLGDLYSGDAYAGNGAYYRALA
                     DVNKQIDSTTAVRLNLMATSNHVVDRDQVYSHRWGADASVAFGLGTDTKLILNYLHQD
                     DNRIPDYGIPVAQTPGVLVMTPASDNGVPRNSFLGYDTDRDETHADVFTERLSHKATP
                     WLTLTSDTRGGFYSRYFQYTTVDRCDATVATAFCATQLFGVNPQAAYGGIGGGGPYSM
                     RAWGLQNVSTARADFNAGMFRTELIAGIDVSYQDNKKNFYYYTLPSAGQFAYVLGNNT
                     ASRANIGRNLFAPDPNPPPNYAPFVYNTAGLNCTVNPCTLSSLVTTATPTSSGRTDSD
                     SSDLGAFLTDRLWFTDELSVIGGLRFDRYNSEFNNFPISGTATQLKSDSTLVSPRASL
                     VYEPDTTKTFYFTWGRAARPQGANVVGDATAIALTTKDLEPEITDSYELGTKYGFLDG
                     RLAVTASLFHEEKNNATQTDPGTGFLVAQSGEKQRVQGFELGLTGKVFDVLTLNAGWS
                     HLDSRTEDSFATCATLTATSTTGVACPAGVAVGTTVPNLAIIGRQVIFVPKDAGSFWA
                     SYDAAEFAPGLSFGGGLTYQSKMPVRYNVLSPLGTPTLASIAEIPDTVSLDAFIAYKF
                     GAYRVSINGYNLTDRLNYVQAFGNRGVPAPGRTVILSVGFTY"
     gene            5703..6386
                     /locus_tag="VGM72_00140"
     CDS             5703..6386
                     /locus_tag="VGM72_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004775794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HEY4263697.1"
                     /translation="MLIRIPDVLTREEVAECRRTLASAEWVDGKITAGAQSAQAKFNL
                     QVPEAAPQARQLGEIILRALGPNLLFNAAALPLRVFPPLFNRYDEGMVFKPHIDNAIR
                     YVTHANIRLRTDIAVTLFLSDPSEYDGGELILEDTYGAQTVKLPAGHMVLYPATSLHR
                     VTPVERGARWASFFWVQSMVKDDGKRRELFELDRAIMETRGILGDTHHAVVALTGTYH
                     NLLRRWSEL"
     gene            6383..7594
                     /locus_tag="VGM72_00145"
     CDS             6383..7594
                     /locus_tag="VGM72_00145"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEY4263698.1"
                     /translation="MSAIGRGQNLSPRRLMFWLHIWVGLAFCVPFALLGITGSLLVYE
                     QPIDDVFWPQSQATASGVMHSPQAIIEAAAKARAGLAANSLSLPQEAGDPAVVRLTER
                     GAGRGGPRGPNYQALIDPVSLQVLDLHLGARSAFMGWVHDFHGSLLIGGREGRSVIGW
                     MGVGMLALGLSGIVLWWPRRNRWRDAYGVKKGARGWLFHRQLHGTTGITAWLLFIVLS
                     FSGTAIAFPQTTASFVRVILFQGPSTAAPAAGPGAPAIRVAPVEGQERIDADKALAIA
                     MAAVPGSHLVTMFLPVGREQPYRLFLLPNGAAQGSPPVAVFVDPYRAQTISVRNPWAG
                     DLGDSVITWQRPLHTGRGTNAVYRASIFLVGLLPALFAITGAGMWWTKRRARARSAAD
                     SDAILQGVAAE"
     gene            7591..8337
                     /locus_tag="VGM72_00150"
     CDS             7591..8337
                     /locus_tag="VGM72_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY4263699.1"
                     /translation="MNVYTPGAAYPLTEQWRETPHALADFARPETAPRNRAKSRFGGI
                     GGVLAIHGLVLAAYVFAGPAFVRIQTQHMLTVVNVFAEPPKPDPPPLLPKLAPPVVYV
                     PVPIVPEIQLAVPPPPRETITIPPAPAKASPPDAPFVPAGPPPPKPTLSATSQAAFAA
                     RLFAHLNRFKRYPEGAKLRHQEGIVSLRFTMDRNGRVLSFDIAKSSGSAALDAEAREL
                     IQRAQPLPALPPEFGRETLDLIVPVEFSLH"
     gene            8410..10257
                     /locus_tag="VGM72_00155"
     CDS             8410..10257
                     /locus_tag="VGM72_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEY4263700.1"
                     /translation="MMANKWALSRGRTDKREAGLGWRDQMRVLRRVAPLLWPKGEPAL
                     RLRVAAALSLIFVGKLVNVALPIVYKWVIDAISMGPRAALTVPVALIVGYGVVRIASA
                     ATTELRDMIFAKVQERALRLVSVSVLEHLLNLSLRFHLDRQTGGLSRSIERGTESIES
                     LLTYLLFSIAPIFLELVLVTGVLWHFFNFELAASIFAVVIFYAAYTAGSTQWRMRHRR
                     EMNTQDREANARAIDTLLNFETVKYFGNERHELDRFDEAKQDYVVAAIANQRSLSLLN
                     IGQSSILSVGVILVMLLAGYGVVAGRMTIGDFVMVNAYLLQLYQPLNMLSWVWRILRQ
                     AFTDIEQMYALLAENSEVRDRPDAPRLRPGSGRVVFDNVSFSYDPRRPILDDVSFEVP
                     GGRTVAIVGPTGGGKSTIARLLFRFYDPQEGAIRIDNQDLREITQESLRAAIGVVPQD
                     TVLFNDSIYYNIAYGRPDASPEDVAEAARRAQLKNFIDKLPDGFATRVGERGLKLSGG
                     EKQRVAIARVLLKNPEILIFDEATSSLDSRTEAEIQTNLRQVSAHHTTLIIAHRLSTV
                     IDADQILVLDDGGIIERGTHDELIARGGTYAAMWDRQQRAAERLADVAE"
     gene            complement(10314..>11951)
                     /locus_tag="VGM72_00160"
     CDS             complement(10314..>11951)
                     /locus_tag="VGM72_00160"
                     /inference="COORDINATES: protein motif:HMM:NF016859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263701.1"
                     /translation="IVKRGREITEDTAEKIETSHVQKVRVRSVLTCELPEGVCGKCYG
                     RDLARGTSVNIGEAVGVIAAQSIGEPGTQLTMRTFHIGGAAQVAAQSKIEASHDGKAK
                     IVNRNVVRNSQGQLVAMGRNMQVAIVDQRGNERATHRITYGARLMVDEGQNIKRGERI
                     AEWNPNSLPILTEVDGIVRYDDISEGLTLTEVADETTGVSQKKIIDWRAGSSKSSDLR
                     PAISIVDKKGKPVATATGEAHYALSVDAILSVEDGSEVKAGDVLARIPTEGAKTRDIT
                     GGLPRVAELFEARKPKDCAVLAETDGYIEFGKDYKNKRRVILKPKNDKQEAVEYLLPK
                     AKHVSVREGDYVEKGDFIIEGHPSPHDILRIKGVEELAVYLINEIQEVYRLQGVKIND
                     KHIEVIVRQMLQKTEIEDGGETTLLPGEQVDLDELEDANKKALEMNGRPARGRPILLG
                     ITKASLQTRSVFSAASFQETTRVLTDAAVNGKVDTLEGLKENVIVGRLIPAGTGGAIA
                     RIRRIAAERDKAIQAERAAAAPPLAIEGPAPEQTAAE"
BASE COUNT         2129 a         3810 c         3528 g         2484 t
ORIGIN      
        1 gtctgcttct tatgcgtgta gtcgacggtg acagacttcg acagcgtttc gcgataggcg
       61 acctgcggtg cgccgacggt cgcgtccacc ttgtaggtgc gccgcaaaat gtcgactttg
      121 atctcgaggt gaagctcgcc catgcccttg agaatcgtct gaccggaatc cagatcggtg
      181 gagacgcgga aagacgggtc ttcctgggcc aggcgattga gcgcgacacc gagtttttcc
      241 tgatcggcct tggtcttggg ctcgatggcg acttcgatga ccggatcggg aaactccatg
      301 cgctccagga tcaccgggtg ctgcggatcg cagagcgttt cgccggtcgt cgtgcccttg
      361 agtccggcaa gggccacgat gtcgccggca taagcctctt tgacgtcttc gcgattgttc
      421 gcgtgcatca ggagcatgcg gccgacgcgc tcgcgattgt ccttgatgga attgagcacc
      481 atcgttccgg aattcatcgt gccggaatac acccgcacga aggtgagcga gccgacaaag
      541 ggatcgtcca tgatcttgaa ggcaaggccg gcgaacggcg ccttgtcgtc cggctgcaga
      601 acgatctttt cttcggaatg cggcttcatg ccttcgacag gcggcacatc caacggcgag
      661 ggcagaaaat cgaccacgcc atcgaggagc ggctgaacgc ccttgttctt gaaggcagca
      721 ccacacagaa ccggcacgaa acggaaagcg caggtgccct tgcggataca ggccttgagc
      781 tgatcgacgc tcggcatgtt tccttcgaga taggcttcca gaagcttctc gtcttcttcg
      841 accgcgctct cgaccagctt ggtgtgcagc tcctgcgcct tttcgcgcag gtcttcgggg
      901 atttccattt cctcgtaatc ggccccgagg ttttcttcct tccagatgag cgccttcatg
      961 gcgatcagat cgatgacgcc cttgaaaccc tgctcggcgc ccaccggaat ctgaattacc
     1021 atcgggtggg tgccgaggcg gtcgatcatc atttgcacgc agcggtaaaa atccgcgccg
     1081 gtgcggtcca tcttgttgac gaagcagatg cgcggcacgc gatatttgtc agcctgacgc
     1141 catacggttt cggattgcgg ctcgacgccg gcaaccgcat cgaacaccgc caccgcgccg
     1201 tcgagcacgc gcatggagcg ctccacctcg atggtgaaat cgacatgccc cggcgtatcg
     1261 atgatgttga ttcggtggcc gttccagaaa caggtcgtgg cggccgaggt gatggtgatg
     1321 ccgcgttcct gttcctgctc catgaaatcc atggtggcgg caccgtcatg gacttcgccg
     1381 atcttatggg aacggccggt ataataaaga atgcgctccg tcgtcgtcgt cttgccggca
     1441 tctatatgcg cggcaatgcc gatgtttcgg tagtggtcga gcggggtttg tcgcggcatc
     1501 ggaggttcct aaacgattac cagcggtagt gcgagaacgc gcgattggct tccgccatct
     1561 tgtgcgtgtc ttcgcgcttt ttgaccgccg tgccgcgatt gttcgcggca tccatgattt
     1621 cccccgaaag tcttccctgc atggtcggct cgttgcggcc gcgcgcggcc gtgatgatcc
     1681 accggatggc gagcgcgcgc gaacggtcgg tgcgcacttc aaccgggacc tgataggtgg
     1741 cgccaccgac gcggcgcgac ctgacttcca gcgcagggct gacattcctc agcgcttcgt
     1801 gaaagacgcc gagcggctcc tgcttcgttt tctgctggat ggtgtcgaag gcgccgtaga
     1861 tgatgccttc ggcgaccgat ttcttgccct catacataag agagttcatg aacttggcaa
     1921 gaaccacatc gccgaacttg gcgtccggga tgacctcgcg tttctctgca cggtggcggc
     1981 gtgacatgga atttaacctc ttcgtctctt cctatggatc ccggcgctcg gctctgtctc
     2041 ggccggggtg acattcgctt cggtgaatgt cactttggcc gtttcgctcc gtatacagaa
     2101 cggcgctgct tgcggtcctt gacgccctgg gtgtccagca cgccgcgcag gatgtgatag
     2161 cgcacgcccg gcaaatcctt gacgcgaccg ccgcgaatca gcaccaccga gtgctcctgc
     2221 agattgtgcc cctcgcccgg gatgtagctc accacttcgt agccgttggt gagtcgcacc
     2281 ttcgccacct tgcgtagggc ggagttcggt ttcttgggcg tcgtcgtata gacgcgcgtg
     2341 cagacgccgc gcttttgcgg acaattggtg agagccggca ccttgttgcg cttcggcttc
     2401 tcgcccctgg gcttgcggat cagctgattg actgtcggca tcgttctctt tttccgttcc
     2461 cggccatggc ggcccatccc aaacaacaaa cgtcctaaac aacaaacgtc tcaaacaaca
     2521 acatccgcgg gaccaactcc taagagttga cccgcaatac gcggaggatc cgggcgctca
     2581 ttgcccccgg atcgcgcttg acccgtgctc tacactttct tcaggcagcg tttcgtgtgg
     2641 ttgccgacgg cctgccgcga aggtggggct gtatactttt aggaacccgg ggcgtcaata
     2701 ccaaaaggtc cgggttccta aggggttgcc gcacaagtgc ccccaccctg cgcgtccggt
     2761 ctgcggacgc caaaacgccg caccgtgcct tgtcttcttg aaaatgatct tgcgactaag
     2821 tcgcaagatg cctataagga gcttgggttt aaagggggcg tttatgcgcc accgaccgag
     2881 gggaccaagt gagcagcaag ccaaaaccgg cggatacgca gggggggacc cttccccgaa
     2941 tcgatgccag catgcttctc gcctcgggcc gtgaagtcat tctcgttcac aaggacaccg
     3001 aatacaggct gcgattgacc agcaacgaca agctcatact gactaaatag ccgcgggttt
     3061 tctcgcttcc ccgcggtatg tcccagccag ccccgccgag gttctcctcg aaaacggatg
     3121 ccagccaggt ctcgtgcgag atcggcattt tttcggagaa catcatggca tccagaagcc
     3181 gcaaatcagg cggaatccgc aaatcaggtt ttcgcaagcg aacgcagcat cttcgcgcgc
     3241 caggcgcact agccgcggca ctgggtggcg tgcttttgat gccggcttac gccttagcgc
     3301 aagcgccaaa ctcgaacaca gcggactcga atacagcggg cgcgatcgag ctgggccccc
     3361 tgcgcgtcca ggaccagaac gccaacgcgc tcggcaacga taccggcctc tccgtcctgc
     3421 ccaccacggt ccaggacacc ccccaagcca tcatcgtcat cccccagcag caattgcgcg
     3481 aacagggcgt cacctcgctc gagcaggcgt tgcgcaatgt gccgggaatc acagtgtcca
     3541 tcggtgaggg cggcacgctg aacggcgatc aattcaagat ccgcggcttc gatgctcaga
     3601 acgacgtcta tgtggacggc ttgcgcgatt tcggggtcta tacccgcgac agcttcgcct
     3661 tccaggaagt gcaggtcctg aaaggaccgt caggcaccat gttcggacgc ggcagcaccg
     3721 gcggcgtcat caataccgtc tccaagaccc catttctggg cgatctctat agcggcgatg
     3781 cctatgccgg aaacggcgcc tattaccgtg cgctggccga cgtcaacaag cagatcgatt
     3841 ccacgacggc ggtgcgcctc aacctgatgg cgacgtccaa tcatgtcgtg gaccgcgacc
     3901 aagtctattc gcaccgttgg ggcgcggatg cgtccgtcgc attcgggttg gggaccgata
     3961 cgaaactgat cctgaattat ctgcatcagg acgataatcg cattcccgat tacggcattc
     4021 cggtcgccca aacaccgggc gttctcgtga tgaccccggc gagcgacaat ggcgtgccgc
     4081 gcaattcctt cctgggctac gacaccgacc gcgacgagac ccatgccgat gtgttcaccg
     4141 aacggctctc gcacaaggca acgccctggc tgacgctgac gagcgatacg cgaggtggtt
     4201 tctattcgcg ttatttccaa tacacgaccg tggatcgctg cgatgccact gtcgcgacgg
     4261 ccttttgtgc cacccagttg ttcggcgtca atccgcaagc ggcttatggc ggcatcggcg
     4321 gcggcggacc ttattccatg cgggcgtggg gccttcaaaa cgtctcgacc gcgcgcgcgg
     4381 atttcaatgc cggcatgttt cgcacagaac tcatcgccgg gatcgacgtg tcctatcagg
     4441 acaacaagaa aaacttttat tactacacgc tgccgtccgc cggtcagttc gcgtatgtcc
     4501 tcggaaacaa caccgcaagc cgcgccaata tcggccgcaa tctgttcgcg ccggatccca
     4561 acccgccacc gaactacgcg cccttcgttt acaacaccgc cggactgaac tgcacggtca
     4621 acccatgcac attgagtagt ctggtcacca cggcgactcc gacatcgtcc gggcgcacag
     4681 acagcgattc ttccgatctc ggcgcctttc tcaccgatcg tctttggttc acggatgagt
     4741 tgtcggtgat cgggggcttg cgtttcgacc gctacaattc cgaattcaac aatttcccga
     4801 tcagcggcac cgctacgcag ttgaaatcgg attcgaccct cgtctcgccg cgcgcgagtc
     4861 tcgtttatga gcccgacacg actaagacat tctacttcac ttggggacgc gcggcgcgtc
     4921 cccaaggcgc caatgtggtg ggcgacgcga cagccatcgc attgaccacc aaggatctcg
     4981 aacccgagat caccgactcc tatgaactcg gcacgaaata cggctttctc gacggacgat
     5041 tggcggtgac cgcatccttg ttccacgaag aaaagaacaa cgccacccag accgaccccg
     5101 gtacgggatt cctggtggcc caatcgggtg aaaagcagag agtgcagggt ttcgagctcg
     5161 gcctcaccgg caaagtcttc gacgttttga cgttaaatgc cggctggtcg catctcgaca
     5221 gcaggacgga agactccttc gccacttgcg ccactctgac ggccacgagt accaccggcg
     5281 tcgcctgccc ggcgggcgtg gcagtgggga ccacagtccc caatctcgcc atcatcggcc
     5341 ggcaggtgat cttcgtgccc aaggatgcag gctcgttctg ggcaagctat gacgcggcgg
     5401 agttcgcgcc gggtctcagc ttcggtggcg gtctcaccta tcagagcaaa atgccggttc
     5461 gctacaacgt gctcagcccg ctcggaacgc cgacgcttgc tagcatcgcc gaaattcccg
     5521 acacggtctc gctggatgcg ttcatcgcct acaaattcgg cgcctatcgg gtttccatca
     5581 acggctacaa tctcaccgac cgtctgaatt acgtacaggc tttcggcaat cgtggcgttc
     5641 cggcgccggg ccggacggtc atcctttccg tcggcttcac ttattaagac gcaacaaagg
     5701 ccatgctgat ccgcattccc gatgtgttga cgcgagagga ggtggccgaa tgccgccgca
     5761 ctctcgcgtc ggcggaatgg gtcgatggca agatcacggc cggtgcgcaa tcggcgcagg
     5821 cgaaattcaa cctgcaagtc ccggaagccg caccgcaggc gcgccaattg ggtgagatca
     5881 tcctgcgcgc gctggggcct aatctcctgt tcaacgcggc ggccctgccc ttgcgcgtat
     5941 tcccgccgct gttcaaccgc tacgacgagg gcatggtttt caaacctcat atcgacaacg
     6001 ccatccgcta tgtgacgcac gccaatatcc gtctacggac ggatattgcc gtgactctgt
     6061 ttctttccga cccttccgag tacgacggcg gcgaactcat tctcgaagac acctatggcg
     6121 cgcaaaccgt caaattgcct gccggccaca tggtccttta tcccgccaca agcttgcatc
     6181 gcgtcacgcc agtcgaacgc ggtgcccgct gggcctcctt cttctgggtc cagagcatgg
     6241 tgaaggatga cggcaagcgg cgcgaattgt tcgaactcga ccgcgccatc atggaaaccc
     6301 gcgggatcct gggcgacacc caccacgcgg tggtcgcgct gacagggacc tatcacaatc
     6361 ttttacgccg atggagcgaa ctatgagtgc cataggacgt ggtcagaatt tgtcgccgcg
     6421 ccggctgatg ttctggctgc acatctgggt gggtcttgcc ttctgcgtgc ctttcgcgct
     6481 tctcggcatc accggcagcc ttctggtcta tgaacagccc atcgacgatg tcttctggcc
     6541 tcaatcccaa gcgaccgctt cgggtgtcat gcattcgccg caggcgatca tcgaggccgc
     6601 cgcgaaagca cgcgccgggc ttgccgcaaa ctcactatcg ctgccgcaag aggccggcga
     6661 tccggccgtc gtgcgtctta ccgagcgcgg cgcgggacgc ggtggtccca gaggcccgaa
     6721 ctatcaggcg ctgatcgatc cggtttcctt gcaggtgctg gatctgcact tgggcgcgcg
     6781 ttcggccttc atgggttggg tgcatgattt ccacggcagc ctcctgatcg gcgggcgtga
     6841 aggccgctcg gtcatcggat ggatgggtgt gggcatgctg gcgctcggtc tttccggcat
     6901 cgtgctgtgg tggccgcgcc gcaaccgctg gcgcgacgct tatggcgtca agaaaggcgc
     6961 gcgcggctgg ctgttccatc ggcaattgca cgggacaacc ggaatcacgg cgtggctgct
     7021 gttcattgtg ctcagcttct ccggcacggc cattgcgttt ccgcagacga ctgcatcatt
     7081 cgtgcgcgtg atcctgttcc agggcccgag cacggcagca ccggccgccg gtccgggcgc
     7141 gcccgcgata cgcgttgcgc cggtcgaagg acaagagcgc atcgacgcgg acaaggctct
     7201 cgccatcgcg atggcagccg tgcccggttc acatctggtg acgatgttcc ttcccgtggg
     7261 gcgcgaacag ccataccgcc tgtttcttct tccgaatggc gcagcccagg gttcgcctcc
     7321 cgtcgccgta ttcgtcgatc cctatcgcgc gcaaaccatc agcgtgcgca atccctgggc
     7381 cggcgatctc ggcgattcgg tgataacgtg gcagcgtccc ttgcacaccg ggcgcggcac
     7441 gaatgcggtt taccgcgcgt cgatcttcct ggtggggttg ctgccggccc ttttcgcgat
     7501 aaccggtgcc ggcatgtggt ggacgaaacg ccgtgcgcgc gcgcgtagcg cggcagacag
     7561 cgatgccatc ctccaggggg tggcggcgga atgaacgtgt acacccccgg cgctgcctat
     7621 cctctgaccg agcagtggcg cgagacgccg cacgcgctgg cggattttgc acggcccgaa
     7681 accgcccctc gcaaccgcgc aaaatcgcgc ttcggcggca tcggcggtgt tttggcgatc
     7741 cacggtctgg tgcttgcggc ctatgtcttc gcaggaccgg ccttcgttcg tatccagacg
     7801 cagcatatgc tgacggtggt caacgtgttt gcggaaccac ccaaaccgga ccccccgccg
     7861 cttttgccga aactggcgcc gccggttgtc tatgtgccgg ttccgatcgt gccggaaatc
     7921 cagctcgcgg ttcccccgcc cccgcgcgaa acgatcacca ttccgcccgc gccggcgaag
     7981 gcttcgcctc ctgatgcgcc ctttgtgccc gccggaccgc cgccgccaaa gcccacattg
     8041 tcggcgacca gccaggctgc cttcgcggcg cgtctgttcg cccatctcaa ccgcttcaag
     8101 cgttatccgg aaggcgccaa gctgcgtcat caggaaggca tcgtctcgtt gcgcttcacg
     8161 atggaccgca atggccgcgt tctatccttc gacatcgcca agagttccgg ttccgccgcg
     8221 ctggatgccg aagcgcgcga gcttatccag cgtgcacagc ctttgcccgc cttgccgccc
     8281 gaattcggcc gcgaaacgct cgatctcatc gtgccagtgg aattttccct gcactgagaa
     8341 acttgccggt gtgcggttga ggagcgtccc ccggcatacg atataacggg gtgcgggttt
     8401 cggattttca tgatggcgaa taaatgggcc ctgagccgcg ggcgcactga caaacgcgag
     8461 gcaggcctgg gctggcgcga ccagatgcgg gtgttgcggc gcgtcgctcc gttgctttgg
     8521 ccaaagggcg aacctgcatt gcgtctgcgg gtggcagccg cgctgtcgct catcttcgtt
     8581 ggcaagctcg tgaatgttgc gctgcccatc gtctacaaat gggtgatcga tgcgatctcc
     8641 atggggccgc gagcggccct gacagtcccc gttgcgctta tcgtaggcta tggcgtggta
     8701 cggattgcat cggcggccac caccgagctg cgcgatatga tcttcgccaa ggtgcaggaa
     8761 cgcgcgctcc ggctggtgtc ggtgagcgtg ctggaacatc tgctcaatct ttccctgcgc
     8821 ttccatctcg accggcagac cggcgggctg tcgcgttcca tcgaacgcgg cacggagagc
     8881 atcgaatcgc tgctcactta tcttctcttc agtatcgcgc cgatcttcct ggaactggtg
     8941 ctcgtcaccg gcgtgctctg gcattttttc aatttcgagc tcgccgcatc gatattcgcg
     9001 gtggtgattt tctacgccgc ctatacggcc ggttccaccc aatggcgcat gcggcaccgg
     9061 cgcgaaatga acacgcagga ccgcgaggcc aatgcgcggg cgatcgacac gctgctgaat
     9121 ttcgagaccg tcaaatattt cggcaatgag cgccacgaac tcgaccgctt cgacgaggcc
     9181 aaacaggatt acgtggtggc agctatcgcc aatcagcgtt ccctgtcgct gttgaacatc
     9241 ggccagtcct cgattctttc ggtgggcgtg atcctcgtca tgctcctggc cggttacggc
     9301 gtcgtcgccg ggcggatgac catcggcgat ttcgtgatgg tgaacgctta tctgctgcag
     9361 ctttatcaac cgctcaacat gctctcctgg gtatggcgca tcctgcgtca ggccttcacg
     9421 gacatcgagc agatgtatgc tttgctggcg gaaaactcgg aggtacggga tcggccggat
     9481 gctccccgct tgcgccccgg atccggccgc gtcgtcttcg acaatgtgag cttttcctat
     9541 gatccgcgcc gtcccatcct ggacgatgtc agtttcgagg tgcccggcgg gcgcaccgtc
     9601 gccatcgtcg ggccaaccgg cggcggcaag tcgaccatcg cgcggctgtt gttccgcttc
     9661 tacgatccgc aggaaggtgc gatccggatc gacaatcagg acttgcgtga gataacccag
     9721 gaatcgctcc gcgcggccat cggcgtggtg ccgcaggaca ccgtgctttt caacgacagc
     9781 atctattaca acatcgccta tggccggccc gatgcgtcgc ccgaagatgt ggcggaggcc
     9841 gcccgccgcg cgcaactgaa aaacttcatc gacaagcttc ccgacggctt tgccacgcgg
     9901 gttggcgagc gaggattgaa gctttccggt ggcgaaaagc agcgcgtggc gattgcgcgc
     9961 gtgctcctga agaatcccga aatcctgatc ttcgacgaag ccaccagttc gctcgattca
    10021 cgaaccgaag cggaaatcca gacgaatctt cgccaagtct cggcccacca tacgacactc
    10081 atcatcgccc atcgcctgtc cacggtcatc gatgccgacc agatcctcgt actcgacgat
    10141 ggcggcatca tcgaacgcgg cacgcatgac gaattgatcg cgcgcggcgg gacctatgcg
    10201 gccatgtggg atcgtcagca gcgtgccgcc gaacgcctgg ccgacgtggc ggagtgacca
    10261 caaaaaaggg ccgcgttttg cgcggccctt ttctttgctt caagacgttg gcgttactcc
    10321 gctgcggttt gttccggagc aggcccttcg atcgccaagg gcggtgcagc tgcggcacgt
    10381 tcagcctgga tcgccttgtc gcgctccgcc gcgatgcgcc ggatgcgcgc gatggcgccg
    10441 cccgtgcccg ccgggatgag ccggccgaca atgacgtttt ccttgaggcc ttcgagcgta
    10501 tccaccttgc cgttgacggc ggcgtcggtg agcacgcgcg tggtctcctg gaaggaggcg
    10561 gccgagaaca ccgagcgcgt ctgcaacgac gccttggtga taccgagcag aatcggacgt
    10621 cccctggcgg gacggccgtt catctccagc gccttcttgt tggcgtcctc caattcgtcc
    10681 agatcgacct gctcgcccgg cagcagtgtg gtttcgccgc catcctcgat ttcggtcttc
    10741 tgcagcatct gacggacgat cacttcgatg tgcttgtcgt tgatcttcac gccttgcaga
    10801 cgatagacct cctggatctc gttgatgagg tagaccgcca gttcctccac gcccttgatg
    10861 cgcaggatgt catgcgggct cggatggcct tcgatgatga agtcgccctt ctccacataa
    10921 tcgccttcgc gcacactgac gtgcttggcc ttgggcagca gatattccac cgcctcctgc
    10981 ttgtcgttct tgggcttcag gatcacgcgg cgcttgttct tgtagtcctt gccgaactcg
    11041 atgtagccat cggtttccgc cagcaccgcg caatctttcg gcttgcgcgc ttcgaacaat
    11101 tcggccacgc gcggcagacc gccggtgatg tcgcgggtct tcgcaccttc ggtcgggata
    11161 cgtgcgagca cgtcgcctgc cttcacttcg gaaccgtctt ccaccgacag gatggcgtcg
    11221 accgacagag cataatgcgc ctcacccgtc gcggtggcca cgggctttcc cttcttgtcg
    11281 acgatcgaaa tggcgggccg caaatccgaa ctcttggacg aacccgcgcg ccagtcgatg
    11341 atcttcttct ggcttacgcc cgtggtttcg tccgccacct cggtcagcgt caagccttcg
    11401 gagatgtcgt cataacgcac gatgccgtcg acttcggtca gaatcggcag tgagttcgga
    11461 ttccattccg cgatacgctc gccgcgcttg atgttctgac cctcgtccac catcaggcgc
    11521 gcgccgtaag tgatccggtg cgttgcccgt tcattgccgc gctgatcgac gatcgcaacc
    11581 tgcatgttgc ggcccatggc cacgagctgg ccctggctgt ttcgcaccac gttgcggttg
    11641 acgatcttcg ccttgccgtc atgggaggct tcgatcttcg actgtgcggc aacctgcgcc
    11701 gcaccgccga tatggaaggt acgcatggta agctgagtgc cgggttcgcc aatcgactgc
    11761 gccgcgataa cgccgacggc ttcgccgata ttgaccgacg tgccgcgcgc cagatcgcgc
    11821 ccatagcatt tgccgcagac gccctcggga agctcgcagg tcaggacgga gcgcacgcgc
    11881 accttctgaa catgcgatgt ctcgatcttc tccgcggtgt cttcggtgat ctcacgcccg
    11941 cgcttgacga t
//
LOCUS       DASYEG010000005        15579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1161214, whole genome shotgun sequence.
ACCESSION   DASYEG010000005 DASYEG010000000
VERSION     DASYEG010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 15579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15579
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161214"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..578)
                     /locus_tag="VGM72_00165"
     CDS             complement(72..578)
                     /locus_tag="VGM72_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263702.1"
                     /translation="MKAKTIIVNDRMQRNYRYERVAPEGRSFDPAFRPGLTPKQMLRL
                     GVFGGKYLTDCVKEFPPLWFVRAKLSPSRRDPALNFFGVDASQPLSEWRRKGWIHPDD
                     PRGWFQWYCRYYMGRRMEDDARQIGRWKAMRRHIRQIEKHCEPGDCFCRPRQRQALLH
                     WAYDARKF"
     gene            complement(595..1176)
                     /locus_tag="VGM72_00170"
     CDS             complement(595..1176)
                     /locus_tag="VGM72_00170"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HEY4263703.1"
                     /translation="MANRAFGFAAFVLGLLLVNAPLAGANPDFDLGPPVNAKAPDIGA
                     PPDQTGTPRTMNSLMGEKGLVLFFFRSAGWCPFCQAQMMDLNTGVAAMEKRGYKLAGI
                     SYDQPDVLKTFIDRRKIAYTLLSDPKSEVIDRYKLRDPQYPPGNFAYGVPRPIIFILD
                     KNAIIKAKLYEDTYTKRPPTTLVIETLDKLAAK"
     gene            complement(1263..1637)
                     /locus_tag="VGM72_00175"
     CDS             complement(1263..1637)
                     /locus_tag="VGM72_00175"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEY4263704.1"
                     /translation="MAKDTNRQVWSAMGRGVRACCPNCGCSPLFRAYLKPVDACAHCS
                     EVFKHIRADDGPAWLTILIVGHIVVGLALTMEIYAPMPVAASIALFCGLSLAMILWLL
                     PRAKGVFIGAIWAMKAPGSELP"
     gene            complement(1795..2403)
                     /locus_tag="VGM72_00180"
     CDS             complement(1795..2403)
                     /locus_tag="VGM72_00180"
                     /inference="COORDINATES: protein motif:HMM:NF004813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY4263705.1"
                     /translation="MAPNGRNTRPMNRFDLTARLVSKLKHDEAVIGGIGNSNFDLWHA
                     GHRPQNFYMLGSMGLATPIALGVALAQPQRRVFALEGDGSLLMQLGALATIANLSPRN
                     LTIIVWDNAMYQITGAQPTATAGRADLVEIARAAGIVQSAWANDEDEFDRLSLRTLSE
                     DGPWFIGARIDGQSPVKQTPREPVIIKNNFMRGLGVQPPSKS"
     gene            complement(2409..2948)
                     /locus_tag="VGM72_00185"
     CDS             complement(2409..2948)
                     /locus_tag="VGM72_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY4263706.1"
                     /translation="MSLSPMPSPAPIDAHWHEAVIRALKDNAVRFVVYVPDNVLRPLI
                     EAVHADDYFTAFTAAREEEAVGIVSGLAMAGSRGIVLMQTSGFATLANVLASLCIAAQ
                     IPALMMVSERGTLGDFQIGQAIVCRTMRPILETLGIEHHSVTRLEDTHFIVDRTIRQA
                     FLTQAPAALILSPLLTAKR"
     gene            3075..4553
                     /locus_tag="VGM72_00190"
     CDS             3075..4553
                     /locus_tag="VGM72_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEY4263707.1"
                     /translation="MAMRNYERLYIGGEWVAPHSGRAIASIDPSTEEVWAEIAEADET
                     DVDRAVAAARAAMAGPWRTKITASQRGALLHKLAELVRRDAIRLAELESRDNGKPLRD
                     TVGEVQRAVDWLVFFAGAADKIAGEQIPFKPGALAYTRREPVGVIGAILPWNSPILLY
                     SWKIGPALAVGNALVVKPAEQTPVTALEIAKLVEEAGFPAGVFNVVPGFGATAGAALS
                     SHRGVNKISFTGSHATARKIMALASANLKRCTFECGGKSPHIVFADADVERALVVATQ
                     SAFRSTGQSCSLGSRVFVQRPLYESFLGRLAERAARIRIGMPLDRATHIGPQTSADQL
                     AKTQNYIAIGKQEGARLVTGGGRPKGFPRGYFVEPTIFADVDNRSRLAQEEIFGPVIA
                     VMPFDTEEEVVRLANDVEYGLVAGLWTSDVGRAHRVASVIEAGLVTVNTFRTTHPMLP
                     YGGFKLSGLGRENGMEALHHFTEVKTVVVDFDAAPPADPFAD"
     gene            complement(4550..13285)
                     /locus_tag="VGM72_00195"
     CDS             complement(4550..13285)
                     /locus_tag="VGM72_00195"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEY4263708.1"
                     /translation="MAAFPKTPQEPIRSELFSVERLESHAESLAEAQIVVGRYSAGRP
                     ISSRLSDNAKILTLAYRIIAEATQDGRPTTPAAEWMLDNFHVVEEQIREVKNDLPPRF
                     YHELPKLAGGHLEGYPRVFGLAWNFIAHTDSRFDLQTLVRFVAAYQRVQPLTIGELWA
                     ITITLRIILVENLRRLAEAIVRNLEERKQADALADRLLGEGGQVSEAPAELLHKYRQS
                     VLPLAFAAQLDHRLRDQDPAVAPAHQWLVERLAAQGTTPDRAIQDELRLHGALNVTVR
                     NVITSMRMISETDWAVFFESVSLVDAELRADSEFEAMDFATRNLYRLAIERLARGSGK
                     TELEVARRAIARAKKEALAAPEGKLSDRERDPGYYLIARGVRRLERDIGFHPPPSEWL
                     TRLNRATGCGGYMGLIVLLTVLVAAIPVIVLMGLGLSGANLLWLGLLGLVPGSEIAIA
                     AVNRMVTRRIGPAILPGMELADGVPAHLRTMIVMPVLLTKPAQVRELIEQLEVHHLAS
                     SDGDLYFALLSDWADCENETAPSDGAILDEAIQGIAQLNARYGPAASGARFRLLHRHR
                     VWNESENRWIGWERKRGKLHELNRLLRGATDTGFLHLDGKPPEVPPDVRYVITLDADT
                     RLPREAAKRLIGKMDHPLNRPRLDPKSNRVVEGHAVLQPRVTPSLPVGFEGSLFQRVF
                     SSPRGLDPYAFAVSDVYQDLFEEGSYTGKGIYEVDTFEAALDGRIPENSVLSHDLLEG
                     IFARAGLVSDIEVVEEFPSRYDVAAARQHRWVRGDWQLLPWILGFGRDSHGHRRNVIP
                     LIGRWKMLDNLRRSLLAPATLLAFLYGWMLPYGASELWMVFILATIMVPSLLPWFAGL
                     VPHRRGGAKRNHLRGVAADFAVSAIQIAFMIIFLAHQAWVMLDAVTRTLFRLLRRRHL
                     LEWVTAAQISRSMHDHWPGVLFQLAVSFAFAVAALVLFHVRGHGTEVLAAPFVTLWAF
                     SPVIAWWSSRSPPSVGRLPVTLADAKTLRKAARRTWRFFESFVTREDHHLPPDNFQQD
                     PKPVVARRTSPTNIGLYLVAIFSARDFGWLGTLDAVERLEATLESMKALEQFNGHFYN
                     WYATDNSAPLHPKYVSTVDSGNLAGHLVVVAQGCREMARFPLKPALRLAGVRDCFDLA
                     CEALDALTRRLRAPAANMKRLGAALDSISALLEAASRKGLDPLLAELVELSDAAAAVA
                     QSLADEARAAEADETLVWVEAMSATLRSHARDLEAFQAEASHPANHDLAEVLGLRTLS
                     PVPVTAATGNGDTQTLVRASQTETASPTDSPSSDSDPLSPALANPALANPALANPAKL
                     GLAARLNVLADTASGMYTAMRFDFLYDSTRQLFSIGYRVDEAALDPSCYDLLASEARL
                     ASFIAIAREDAPPKHWFRLGRTLTPVGHGAALISWSGSMFEYLMPSLVMRAPAGSILD
                     QTNRIVVRRQIEYGRQLGVPWGVSESLFNARDIEGTYQYAGFGVPDLGYKRGLSENVV
                     IAPYATALAAMIDPAAAAENFRRLTDEGGLGDYGWYDALDYTATRLPKGAKVGIVRAY
                     MAHHQAMSIVAIADALLGAEMRDRFHADPSIQATELLLQERMPRDVAVARPPAQKIRS
                     SAMVQQIAPEMKRQFNSPHSHIPRTHLLSNGTYAVMVTGAGSGYSRWRNIDVTRWRED
                     VTCDDWGSYIFLRDTRSGETWSAGYQPTGMEPDFYEVSFFEDRAEFVRQDGTVKTMLE
                     VAVSPENDGEVRRVSITNLGTRTREIELTSYAELVLAPYGDDATHPAFSKLFVETEYV
                     AGAGTLLATRRRRSPDQAEVWAAHLVVIEGDADSGLQFETDRARFLGRGQRIRNPQCV
                     MEGWPLSNTVGCVLDPIFSLRCRVRVPRGETVRLSFWTVVAPSRQEALDLADKHRDPT
                     AFDRVGTMSWTQVQGQFHYLGIDPAEAHLFQRLANRVIYSDPALRPASEALKRGGRNI
                     SALWAYGISGDLPIVLVRIDRDDDIDLVKQLIRAFEYWRIKRLSVDIVILNERATSYL
                     QTLQETIEHLVRPMQSRPKGPDDVRGDVFVLRSDLVPQDIAALLHTVARMMVLSRRGT
                     LAEQLSRIEEPRPPVIALPRRPSASLPAPDSTRRRPAMEFFNGMGGFAAEGREYVTVL
                     ERGQSTPAPWINVISNENFGFQVSTDGSGFTWSVNSQQNRLTPWSNDPVGDPPGETIF
                     LRDEDSGELWTPTALPIREEGASYLVRHGQGYSRFEVDLHGLALDLVQYVPLNDPVKI
                     SRLKITNNSRQVRNISVTSYVEWVLGPTRAATAPYIVTEIDPETGAMFARNPLSNDYG
                     TRVAFTDLDGRQSSWTGDRTEFLGRNRTLRRPIALLRGTALSSRTGAGFDPCGALQTS
                     LRLKAGSSIEVVFLLGEAASTAEAQRLITKYRTADLNAVLAEATEFWDEALSSVQVKT
                     PDRALDIIINRWALYQALACRVWSRSGFYQSSGAYGFRDQLQDSMALAIAKPGIARAH
                     LLRAAGRQFPQGDVQHWWLAETGRGVRTRVSDDRVWLAYVTAHYVRVTGDLGVLDEQV
                     PFLDGPPLQDGQHDAFFQPSVAKDRGRLFAHCAVALDSALSVGGHGLPLMGTGDWNDG
                     MNRVGEDGKGESVWLGWFLYATLTEFSRLADIRDPSRAARWRGHAEKLKTALEKAWDG
                     DWYRRAYFDDGTPLGSVANTECRIDSIAQSWSVLSGAAEPARAARAMAAVEKYLVRRD
                     EGLVLLFTPPFEHAVPDPGYIKGYPAGIRENGGQYTHAALWSALAYATLGDGDRTHEL
                     LTLLNPIQHAGNSAAIHRYKVEPYVICADVYGAAPHTGRGGWTWYTGSAAWMYRVALE
                     SLLGFQKDGETLILNPCIPRAWPGFEIRYRHGRTMYEITVQNRYSAGRGVTHVVLDGV
                     DLGGPDARIPLKDDGATHKVSVTLG"
     gene            13286..13699
                     /locus_tag="VGM72_00200"
     CDS             13286..13699
                     /locus_tag="VGM72_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263709.1"
                     /translation="MGFESTKEERSQGGEIPCVSQMADTCSRKTQDAKKQQRNCHAWA
                     VLGQRLLRRSVPANLTVNSHVRKRNRSNRIDMVRRGNVLRLTLQKKIPLSNITIFVRR
                     GSESRRCFYQTRAPKPAKTRRDRNAIHDADDSKGL"
     gene            13662..14099
                     /locus_tag="VGM72_00205"
     CDS             13662..14099
                     /locus_tag="VGM72_00205"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY4263710.1"
                     /translation="MRFMMLMIPKGYENAGPGTKPDPNAVGAMMKYNEELATAGVLLG
                     LDGLHPPAAGVRVSFAGGKPNVTDGPFAETKEVIGGYWMIRVKSKQEAIAWASRCPAS
                     ENETIEIRQVFEPADFGPEIGKEGTALMEKINRNLDGNRGAKS"
     gene            14194..14607
                     /locus_tag="VGM72_00210"
     CDS             14194..14607
                     /locus_tag="VGM72_00210"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY4263711.1"
                     /translation="MRFMMIVKATRDSEAGVMPKEALMAAMASYHEDLAKAGVLLDAS
                     GLQSSKKGWRIQFANGKRTVVDGPFAETKELIAGYTILQVKTREEALEWAKRMPNPTG
                     DKDAEIEVRQLFELEDFAPGEAIERLRELETGLKK"
     gene            14667..15083
                     /locus_tag="VGM72_00215"
     CDS             14667..15083
                     /locus_tag="VGM72_00215"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4263712.1"
                     /translation="MYVQPYLFFDGKCEEALNFYKAALGAEVRAMMRFSESPQPAPPG
                     MIPPGSENKVMHASFRIGDTILMASDGNAKGNPVFQGVSLSVTVTNDADAQRVFGALS
                     AGGHVQMPLAKTFFASSFGMVADRFGVSWMVIAGQP"
     gene            15244..>15579
                     /locus_tag="VGM72_00220"
     CDS             15244..>15579
                     /locus_tag="VGM72_00220"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY4263713.1"
                     /translation="MAYMLLILEGHDDRKKRGRDEGRVAYDSMMRFAGDLSARGLLKG
                     ANALRSDAQGVRVAVRDGRQSIVDGPFAESKEMVGGFFLLDCATREEAIAIAQACPAA
                     KWATVEVREV"
BASE COUNT         3069 a         4776 c         4938 g         2796 t
ORIGIN      
        1 cgggcgaggt gcgcgtgctg ttcgagatag aacccaccgc tgtcagcggc aataaatagg
       61 cgtgaaagac ctcagaattt acgggcatca taggcccaat gcagcagcgc ctgacgctgg
      121 cgcggccggc agaagcaatc gcccggctcg cagtgttttt cgatctgccg gatgtgacgg
      181 cgcatcgcct tccagcgtcc gatctgccgc gcatcgtcct ccatgcgccg tcccatgtaa
      241 tagcggcaat accattgaaa ccagccgcgc ggatcgtcgg gatgaatcca acccttgcgc
      301 cgccactccg ataggggctg gctcgcatcg actccgaaga aattcagcgc aggatcacgt
      361 cgtgaaggcg aaagtttcgc gcggacaaac cagaggggcg gaaattcctt gacgcaatcg
      421 gtcaggtatt tgccgccgaa aacgccgagc cgaagcatct gcttcggcgt gaggccgggg
      481 cgaaatgcgg gatcgaacga tctgccttcc ggagcaacgc gctcatagcg ataattgcgc
      541 tgcatcctgt cgttgacgat gattgttttg gccttcacgc cgagggccgg cgtcttattt
      601 cgccgccagt ttgtccagcg tctcgatcac cagcgtggtg ggcggacgct tggtataggt
      661 gtcttcgtag agcttcgcct tgatgattgc gttcttgtcc agaatgaaga tgatcggacg
      721 cggcacgcca taggcgaaat tgccgggcgg atattgcgga tcgcgcaatt tgtaacgatc
      781 gatcacttcg gatttcggat cggaaagcag cgtataggcg atcttccgcc ggtcgatgaa
      841 ggtcttgagc acatcgggtt ggtcatagga gatgccggcg agtttgtagc cacgcttttc
      901 catggccgcg acaccggtgt tcaaatccat catctgcgcc tggcagaacg ggcaccagcc
      961 cgcggagcgg aagaagaaca gcaccaggcc cttttcgccc atcagcgaat tcatggtgcg
     1021 cggcgttccg gtctgatcgg gcggtgcgcc gatgtccggg gccttggcgt tcaccggcgg
     1081 acccagatcg aagtccggat tggcgcccgc gagcggggcg ttcacaagca gcagccccag
     1141 gacgaacgcc gcaaaaccaa aagcgcggtt tgccatgagc caatctccga tcatcggcgc
     1201 gacgctactc ccaagcgccc gattgcggca agcaagcgcg ggcgcggcgg cggccgattt
     1261 tcttatggta attcggaacc gggtgccttc atggcccaga tggcgccaat aaagacaccc
     1321 tttgcgcgcg gcagcagcca caggatcatc gcgagcgaca aaccgcagaa cagcgcaata
     1381 ctcgccgcga cgggcatggg agcatagatt tccatggtca atgcgagacc gaccacgata
     1441 tgtccgacaa taaggatggt gagccaggcc ggcccgtcat cggcacggat gtgcttgaac
     1501 acttcgctgc aatgcgcgca ggcatcgacc ggcttgagat aggctcgaaa aagcggcgaa
     1561 catccgcaat tcgggcagca ggcccgcacg ccgcgcccca ttgccgacca aacctgtctg
     1621 ttcgtgtcct tcgccatgcc cggttccttc cagagcaact cgatgcgatg tggggcagtt
     1681 tagcaaccaa acaatagcca aatgcttagc attttgccgc agagggggcg ttggattgcc
     1741 gcaccgcgct cttggctcgt tcgacttttg ggcccattca acttttaggc cccttcagct
     1801 tttggatggc ggctgaacgc cgagaccgcg catgaaattg ttcttgatga tcaccggttc
     1861 tcgcggcgtc tgtttgaccg gcgactgccc gtcgatacgc gcgccgatga accaaggacc
     1921 gtcttcgctg agcgtgcgca agctcagccg gtcgaattcg tcctcatcgt tcgcccaagc
     1981 gctctggacg attccggcgg cgcgcgcgat ctccaccaga tcggcgcggc ccgccgtcgc
     2041 ggtcggctgc gcgccggtga tctgatacat cgcattgtcc cagacgatga tggtgagatt
     2101 gcggggactg aggttggcaa tggtcgccaa tgcgccgagc tgcatcagca gcgagccatc
     2161 gccttcgagc gcaaagacgc gccgttgcgg ctgggccagc gccacgccca atgcgatggg
     2221 cgtggcgagg cccatgcttc ccagcatgta gaaattctgc ggccggtggc cggcgtgcca
     2281 gagatcgaaa ttggaattgc cgattccccc gatcaccgct tcgtcatgtt tcaatttcga
     2341 caccagccgc gcggtgagat cgaagcggtt catcggcctg gtgttgcgtc cgttcggggc
     2401 catgtctttc accgtttcgc ggtcaggagc ggcgaaagga tcagggccgc cggcgcctgg
     2461 gtgagaaacg cctggcgtat cgtgcggtcg acgatgaaat gggtgtcctc aagccgcgtg
     2521 acgctgtgat gctcgatgcc cagcgtttcc agaatgggac gcatggtgcg gcagacgatg
     2581 gcctgcccga tctggaaatc gcccagcgtg ccgcgctccg agaccatcat cagcgcgggg
     2641 atctgcgccg cgatgcaaag tgacgccaac acattggcga gcgtcgcaaa gccgcttgtc
     2701 tgcatcagca cgatgccgcg cgatcccgcc atggcgagtc ccgaaacgat gccgacggct
     2761 tcttcctcgc gcgccgcggt gaaggcggtg aaataatcat cggcgtggac ggcctcgatc
     2821 agtggacgca gcacattgtc gggaacatag acaacgaaac gcaccgcatt gtccttgagc
     2881 gcgcggatga ccgcctcatg ccaatgggca tcgataggtg cgggggaagg catgggcgaa
     2941 agcgacatca atgggctccg gatcggattc tcacatatcg cccgatgcct cgaacaggcg
     3001 caatttgact tgagggacga aattctcaag gatttttcaa aagccggcga tcgggccagc
     3061 aaaaagggac ggcaatggcg atgcgaaatt atgagcggct ttatatcggg ggagagtggg
     3121 tggcgccgca ttccgggcgc gccatcgcat ccatcgaccc ttcgaccgag gaggtctggg
     3181 ccgagatcgc cgaagccgac gagaccgatg tggaccgcgc ggttgcggcg gcgcgcgcgg
     3241 ccatggccgg tccgtggagg acgaaaatca ccgcgagcca gcgcggcgcg cttctccaca
     3301 aactcgcgga actcgttcgc cgtgacgcca tccggctggc ggagcttgaa agccgtgaca
     3361 atggcaagcc tttgcgcgac acggtgggag aggttcagcg tgccgtcgat tggctggtgt
     3421 tctttgccgg tgccgccgac aagatcgcgg gtgaacagat tccattcaaa ccgggcgcgc
     3481 tcgcctatac gcgccgcgag cccgtgggcg tgatcggcgc catcctgccg tggaattcgc
     3541 cgatcctgct ctattcgtgg aagatcggcc ccgctctggc ggtcggcaac gcgctcgtcg
     3601 tgaagcccgc cgaacagact ccggtgacgg cgctggagat cgccaaactg gtcgaggagg
     3661 ctggtttccc ggccggcgtg ttcaatgtgg ttccgggttt cggcgctacg gcgggcgcgg
     3721 cgctttccag ccatcgcggc gtgaacaaaa tctcgttcac cggcagccat gcgaccgcgc
     3781 gcaagatcat ggcgttggcc tcggccaatc tgaaacgctg cactttcgag tgcggcggca
     3841 agtcgcccca tatcgtcttc gccgacgccg atgtggagcg cgccctcgtc gtggcgacac
     3901 aatcggcctt ccgctccacc ggacaatcct gttcgctcgg gtctcgcgtt ttcgtgcagc
     3961 ggccgctgta tgaatccttc ctcggacggc tcgccgagcg cgcggcccgt atccgcatcg
     4021 gcatgccgct cgaccgcgcg acccatattg ggccgcagac ttccgccgat cagcttgcga
     4081 agacgcagaa ttacatcgcc atcggcaaac aggaaggcgc gcgtctcgtc accggcggcg
     4141 gacggcccaa aggatttccg cgcggctatt tcgtcgagcc cacgatcttt gccgatgtcg
     4201 acaatcgctc gcgcctcgcg caggaagaga tttttggccc cgtcatcgcg gtcatgccat
     4261 tcgatacgga agaggaggtc gtccgcctcg ccaacgacgt ggaatatggg ctggtcgcgg
     4321 gcctgtggac gtccgatgtc gggcgcgccc accgcgtggc ctcggtgatc gaagcggggc
     4381 tcgtcacggt caacaccttt cgcaccaccc acccgatgct gccctatggc ggattcaagc
     4441 tgagtggatt gggccgcgag aacgggatgg aggcgctcca tcatttcacc gaagtgaaaa
     4501 cagtcgtggt ggatttcgac gccgctccgc cggccgatcc gttcgccgat taacccagcg
     4561 tgacgctcac tttgtgcgtt gcgccatcgt ccttgagcgg gatgcgggca tcgggacccc
     4621 cgagatccac gccgtcgagc acgacatggg tcacgccgcg ccccgcgcta tagcggttct
     4681 gtaccgtgat ttcatacatc gtgcggccgt ggcgatagcg gatttcaaag cccggccaag
     4741 cgcgcggaat gcagggattg agaatgagcg tttcgccatc cttctggaat cccagcagac
     4801 tttccagggc gacgcgatac atccaagccg ccgaaccggt gtaccaggtc cagccgccgc
     4861 gcccggtgtg cggggctgcg ccatagacat cggcgcatat gacataaggc tcgaccttgt
     4921 agcgatggat cgcggcactg ttgcccgcgt gctgaatggg attgagcaag gtcagcaatt
     4981 catgtgtcct gtcgccgtcg cccaatgtgg cataggcgag cgccgaccat aatgcggcgt
     5041 gggtgtactg gccgccattc tcgcgaatgc cggccgggta acccttgatg tagcccggat
     5101 cgggcaccgc atgctcaaag ggcggggtga acagaaggac cagtccttca tcgcgcctca
     5161 cgagatattt ttccaccgcc gccatcgcgc gcgcggcgcg cgccggctcg gccgcgccgg
     5221 acaggacact ccacgattgc gcgatcgaat cgatgcgaca ctccgtattg gcgaccgaac
     5281 cgagcggcgt cccgtcgtcg aaataggcgc ggcgatacca atcgccgtcc caggcctttt
     5341 cgagggccgt cttcaacttt tcagcatgac cgcgccagcg cgcagcgcgt gaaggatcgc
     5401 ggatgtcggc gaggcgcgaa aactccgtca gcgtcgcgta gagaaaccat cccagccaga
     5461 cactttcgcc ttttccatcc tcaccgacgc gattcatgcc gtcattccag tcgcccgtgc
     5521 ccatcaacgg caggccgtgc ccgccaacgg aaagagcgct gtccaaggcc accgcacaat
     5581 gcgcaaaaag acgtccacga tcctttgcaa cgctcggctg gaagaacgca tcgtgctggc
     5641 cgtcctgcaa aggcgggccg tccaggaacg gcacctgttc gtcgagtacg ccgagatcgc
     5701 cggtgaccct gacataatgc gccgtcacat aggcgagcca gacccggtcg tccgacacgc
     5761 gcgtgcgcac gccgcgtccg gtttcggcca gccaccaatg ctgcacgtcg ccttgcggga
     5821 attgacgccc agcggcgcga agcaaatggg cgcgcgcgat accgggtttg gcgattgcga
     5881 gcgccatact gtcctgcaac tgatcgcgaa agccgtaggc gccgctcgat tgatagaacc
     5941 ccgaacggga ccacacccgg caggccaggg cctgatagag cgcccagcga ttgatgatga
     6001 tgtccaacgc gcgatccggg gtctttacct gcacgcttga gagcgcctcg tcccagaact
     6061 ccgtagcctc ggccaggacc gcgttgaggt cggcggtgcg gtatttggtg atgagtctct
     6121 gtgcctcagc cgtgctcgcc gcctcgccca gcaggaacac gacctcgatg gaactgcccg
     6181 ctttcaggcg caaactcgtc tgcagcgcgc cgcaaggatc gaacccggct cccgtccggc
     6241 tcgacagggc cgttccccgc aacagcgcga tgggacgtcg cagggtcctg ttacggccca
     6301 gaaattccgt gcggtcgccg gtccagctgc tctgccgtcc gtcaagatcg gtgaacgcga
     6361 cgcgcgtccc gtaatcgttg cttagcgggt tgcgcgcgaa catcgcgccg gtttccggat
     6421 cgatctcggt gacgatgtaa ggcgccgtcg cggctcgggt cgggccgagc acccattcga
     6481 cataggacgt gaccgagatg ttgcgcacct gccgcgaatt gttggtgatt ttcaggcgtg
     6541 aaatcttcac cggatcgttc agcggcacat attgcaccaa gtcgagcgcg aggccgtgca
     6601 ggtccacttc gaaccggctg taaccctgac cgtgccgcac gagataggat gccccttctt
     6661 cgcggatcgg aagcgcggtc ggcgtccaca actcaccgct gtcctcgtcg cgcagaaaaa
     6721 tcgtctcacc cggagggtcc ccgaccggat cgttcgacca tggcgtcaag cgattttgct
     6781 ggctgttcac ggaccaggtg aatccagacc cgtcggtgga aacctggaag ccgaaattct
     6841 catttgaaat cacgttgatc cagggtgcgg gcgtcgattg tccgcgctcc agaacggtga
     6901 cgtattcccg cccctcggcg gcgaaaccgc ccatgccatt gaagaactcc atcgccggac
     6961 ggcggcgtgt cgaatccggc gccggcagac tcgccgaagg ccgccgcggg agggcaatga
     7021 ccggcggcct cggttcttcg atgcgggaga gctgctccgc gagcgtgccc cgccggctaa
     7081 gcaccatcat gcgcgccacc gtatgcaaaa gcgcggcgat gtcctgggga acgagatcgg
     7141 agcgcaggac gaacacgtcg ccgcgcacgt cgtccgggcc tttggggcgc gactgcatgg
     7201 gccgcacgag gtgttcgatg gtctcctgca aggtctggag ataggacgtg gcgcgctcgt
     7261 tcaggatgac gatatccacc gacagccgct ttatccgcca atattcgaaa gcgcgaatca
     7321 gctgtttgac gaggtcgatg tcgtcatcgc ggtcgatccg tacaaggacg ataggcaggt
     7381 cgcccgaaat gccgtaagcc caaagcgcgg agatatttcg cccgccgcgt ttcagcgcct
     7441 ccgaggcggg gcgcaaggcc gggtcggagt agatcacgcg attggcgagg cgctggaaca
     7501 gatgggcttc cgccgggtcg atgccgaggt agtggaactg cccctggacc tgcgtccagg
     7561 acatggtgcc gacgcgatcg aacgccgtcg ggtcgcgatg tttgtccgcc agatcgaggg
     7621 cttcctgccg cgagggcgcg accacggtcc agaacgacag acgcactgtc tccccccggg
     7681 gaacccgcac ccggcagcgc aggctgaaga tcgggtcgag cacgcacccg acggtattgg
     7741 aaagcggcca gccctccatc acgcattgcg ggttgcggat gcgctgacca cgcccgagga
     7801 atcgcgcgcg gtccgtttca aactgaaggc cgctatcggc atcgccttcg atcactacga
     7861 ggtgggctgc ccaaacttcg gcttggtccg gcgatctgcg ccgccgtgtc gccagcagcg
     7921 tgcccgcgcc ggcaacatat tcggtttcga cgaacaattt tgaaaaggcg ggatgcgtgg
     7981 cgtcgtcccc ataaggagcc agcaccaatt cggcgtagga ggtcaattcg atctcgcgcg
     8041 tgcgcgttcc aagattggtg atcgagacgc ggcggacctc gccgtcgttt tcgggcgaca
     8101 ctgcgacttc gagcatggtc ttgacggtgc cgtcctgccg tacgaattcc gcgcggtctt
     8161 caaaaaacga gacttcgtaa aagtccggtt ccatgcccgt tggctgatac cccgccgacc
     8221 aggtttcgcc gctgcgcgta tcgcgcagaa aaatatagct gccccaatcg tcacaggtga
     8281 cgtcctcccg ccagcgcgtg acgtcgatat tgcgccagcg gctgtagccg gaacctgcgc
     8341 ccgtcaccat caccgcgtag gtcccattcg acagcagatg cgttctggga atgtgcgagt
     8401 gcggcgagtt gaactgacgc ttcatctcgg gagcgatctg ctggaccatc gccgatgaac
     8461 gaattttttg cgccggcggg cgggccaccg cgacgtcgcg cggcattctt tcctgcagga
     8521 gaagctcggt tgcctggatc gacgggtcgg cgtgaaaccg gtcgcgcatt tccgcgccca
     8581 ggagcgcatc ggcgatggcg acgatgctca tggcctgatg atgcgccata taggcgcgca
     8641 cgatccccac cttcgcacct ttgggcaggc gcgtggcggt ataatccagc gcatcgtacc
     8701 agccataatc gccaaggccg ccttcgtccg tcaggcggcg gaaattctcc gccgcggcgg
     8761 cgggatcgat catcgcggcc agcgcggtgg catagggcgc gatcacgacg ttctcgctca
     8821 gaccgcgctt gtatccaagg tccggcacgc cgaaacccgc atattggtac gtgccttcga
     8881 tgtcgcgcgc attgaacagg gattcggaga cgccccacgg aacgccgagc tgccgcccat
     8941 attcgatctg cctacgaacg acaatccggt tggtctgatc caggatgctg cccgcgggcg
     9001 cgcgcatcac aagcgatggc atcagatatt cgaacatcga gcccgaccac gagatcagcg
     9061 ccgcaccgtg gccaacagga gtgagggtgc ggccgagccg gaaccaatgt ttcggcggcg
     9121 catcctcgcg ggcgattgcg atgaagcttg ccagacgcgc ctcggaggcg agcagatcgt
     9181 aacagctcgg atcgagagcg gcctcgtcca cccggtagcc gatggaaaaa agctggcgcg
     9241 tcgaatcata gagaaaatcg aaccgcatcg cggtgtacat cccgctcgcc gtgtccgcga
     9301 ggacgttcag ccgcgccgcg aggccgagtt tcgcggggtt ggccagagcg gggttggcca
     9361 gagcggggtt ggccagagcg gggctcagag gatcgctgtc gctggatggg gaatcggtgg
     9421 ggctcgcggt ttctgtttgg gacgcgcgca ccaaggtctg ggtgtcccca ttgcctgttg
     9481 ccgccgtcac aggaaccggg ctgagcgtcc gaagccccaa gacctctgca agatcatgat
     9541 tcgcaggatg cgaggcttcg gcttggaatg cttccagatc gcgggcatgg ctgcgcaatg
     9601 tcgcgctcat cgcttccacc cagacgagcg tctcatcggc ctcggctgcc cgcgcctcat
     9661 cggcgaggct ttgcgcgacc gcggccgccg cgtcggacaa ttccaccagc tccgccagaa
     9721 gcgggtccag cccttttcgc gacgcggcct ccaacagggc gctgatggaa tccagagccg
     9781 cacccagccg cttcatgttg gcggcgggcg cgcgaagacg gcgcgtcagc gcgtcgaggg
     9841 cttcgcaggc aagatcgaag caatcccgca cgcctgccag gcgcagtgcg ggtttcaacg
     9901 gaaacctcgc catttcgcgg caaccctgcg cgaccacgac aagatgacct gccagatttc
     9961 cgctatcgac agtcgacaca tatttgggat gcaaaggcgc ggaattatcg gtcgcgtacc
    10021 aattgtagaa atgaccgttg aactgttcga gcgccttcat gctttccaat gtcgcttcga
    10081 gacgttcgac cgcgtccagc gtgccgagcc atccgaaatc gcgcgccgag aagatcgcga
    10141 ccagatagag gccgatattg gtcggcgacg tgcggcgcgc gacaaccggc ttgggatcct
    10201 gctggaaatt atcgggagga agatgatggt cctcccgcgt gacgaagctt tcgaagaaac
    10261 gccacgtccg ccgtgcggcc ttgcgcagcg tcttggcatc ggccagcgtg acaggaagcc
    10321 gtccgaccga tggcggcgag cggctcgacc accatgcgat gaccggagaa aaggcccaca
    10381 acgtgacgaa cggtgcggcc agcacctccg tgccgtgacc gcggacatga aacaagacga
    10441 gcgcggcgac agcgaatgca aagctgaccg caagctggaa caggactccg ggccaatggt
    10501 cgtgcatgct gcggctgatc tgtgcggccg tgacccattc gagaagatgg cgccgccgca
    10561 aaagccggaa cagcgtgcgc gtcacggcat ccagcatcac ccaggcctga tgggcgagga
    10621 atatgatcat gaatgcgatc tggatggccg aaaccgcgaa atcggcggcg acaccccgca
    10681 gatgattgcg cttggccccg cctctgcgat gcggcaccag ccctgcgaac cacggcagga
    10741 gcgagggcac catgatggtc gcgagaatga acaccatcca gagttcggac gcgccgtagg
    10801 gcagcatcca gccgtaaaga aatgcgagca gggtggcagg cgcaagcaag gaccggcgca
    10861 gattgtccag catcttccag cgcccgatca gcggaatgac gttgcgcctg tgaccatgag
    10921 aatcccgtcc aaagccgaga atccagggca gcaattgcca atcgccccgc acccagcggt
    10981 gttgccgggc ggcggcgaca tcgtagcgcg agggaaattc ttccacgacc tcgatgtccg
    11041 acacgagccc ggcgcgcgcg aagatccctt ccagaagatc gtggctgagc acggaatttt
    11101 ccggaatgcg cccgtcgagt gcggcctcga acgtatcgac ctcgtaaatt cccttgcccg
    11161 tgtaggaccc ctcctcgaac aaatcctgat agacgtcgga aaccgcgaag gcatagggat
    11221 cgagcccgcg gggcgaggag aacacccgct gaaacaacga gccctcgaag ccaaccggca
    11281 acgatggcgt gacgcgcggc tgtagcaccg catggccttc cacgacgcgg ttggatttcg
    11341 gatcgagccg cgggcgattg agcgggtgat ccattttgcc gatcaagcgc ttcgcggcct
    11401 cgcgcggaag ccgcgtatcg gcatcgagcg tgatcacata gcggacatcg ggtggcactt
    11461 ccggcggctt gccgtccagg tgcaggaagc cggtatcggt ggcgccccgc aggagacgat
    11521 tgagttcgtg cagcttgccg cgcttgcgtt cccatccgat ccatcggttt tcgctttcgt
    11581 tccagacccg gtggcgatgc agaagccgga agcgcgcgcc actggcggcc ggcccatagc
    11641 gcgcgttcag ttgcgcgatg ccctgaatgg cctcatcgag aatcgcgccg tcgctgggcg
    11701 cggtttcgtt ttcgcaatcc gcccaatcgg agagcagcgc gaaatacaga tcgccgtcgc
    11761 tgctcgcgag gtggtgcact tcgagctgtt cgatcagctc gcgcacctgt gccggcttgg
    11821 tcagcagaac cggcatcacg atcatggtgc gcaaatgggc gggaacgccg tccgccagct
    11881 ccattcccgg aagaattgcg ggaccgatgc gccgggtgac catacgattg accgccgcga
    11941 tcgcgatctc cgatcccgga acgaggccaa gcagtcccag ccacagcaga ttcgctcccg
    12001 aaaggcccag gcccatgagc acaatgaccg gaatcgccgc aacgagcacg gtcagcaaca
    12061 cgatcaggcc catatagccg ccgcagcccg tcgctcgatt cagacgcgtg agccattccg
    12121 acggcggtgg atggaatccg atgtcgcgtt caagccggcg aacgcctcgg gcgatgagat
    12181 aataaccggg gtcgcgttcg cgatcggaga gcttgccctc gggcgcggcc agcgcctcct
    12241 tcttggcgcg tgcgatcgcg cgccgcgcca cttcgagctc ggtctttccc gatccccgcg
    12301 cgagacgctc gatggcgagc cggtaaagat tgcgtgtggc aaaatccatt gcctcgaatt
    12361 cagagtcggc gcgcaattcg gcgtctacga ggctgacgct ctcgaaaaag accgcccaat
    12421 cggtttcgga aatcatgcgc atgctggtga tgacattgcg caccgtcacg ttgagggcgc
    12481 cgtgcagacg caattcgtcc tggatcgcgc ggtccggtgt ggttccctgc gcggcgaggc
    12541 gctcgaccag ccattgatgc gcgggcgcca cggctgggtc ctggtcgcgg agccgatggt
    12601 ccaattgcgc ggcaaaggcc agcgggagca ccgactggcg gtatttatgc agcaattccg
    12661 ccggggcttc ggaaacctgg cctccttcgc cgagcagacg atccgcgagc gcgtcggcct
    12721 gcttgcgctc ttcgagattg cgcacgatgg cttccgcgag gcggcgcaga ttttccacca
    12781 ggatgatccg caacgtgatt gtgatcgccc acaattcgcc gatcgtcagc ggctgcaccc
    12841 gctgataggc agcgacgaag cgcaccagtg tctgaagatc gaaccggctg tcggtgtggg
    12901 cgatgaaatt ccaagccaga ccgaagactc gcggatatcc ttcgagatgg ccccccgcga
    12961 gcttgggcag ttcatgatag aagcggggag gaagatcgtt cttgacctcc cgtatctgtt
    13021 cttcgacgac atggaaattg tccagcatcc actcggccgc tggcgtggtg ggccgtccgt
    13081 cctgggtcgc ctcggcaatg atccggtagg ccagcgtcag aatcttggca ttgtcggaga
    13141 ggcgtgagga tatcggcctg ccggcggaat agcgtccaac gacgatttgt gcctcggcaa
    13201 ggctctcggc atggctttcg agccgctcca cgctgaaaag ctccgagcgg atgggctctt
    13261 gcggtgtctt cgggaaagcc gccaagtggg atttgaaagc acgaaggaag aacggtctca
    13321 agggggcgaa ataccttgcg tgtcacaaat ggctgatacg tgctcccgga aaacccagga
    13381 cgccaaaaag cagcagcgga actgccacgc ctgggctgtc ctcggacaac gcctgttgcg
    13441 ccgttctgtt ccagcaaacc tcactgttaa cagccacgtg agaaagcgca accggagcaa
    13501 tcgcatcgac atggtgcgcc gcggcaacgt tttacggctc acgttacaaa aaaagattcc
    13561 gctgtcgaac atcaccatct tcgttcgtcg tggaagcgag agcaggcgtt gtttctatca
    13621 aacgcgtgct ccaaaaccgg caaaaacgag gagagaccgc aatgcgattc atgatgctga
    13681 tgattccaaa gggctatgaa aatgccgggc ccggcacgaa gcccgacccc aatgccgttg
    13741 gcgcgatgat gaaatataat gaggagctgg ccacggcggg tgtcctgctc gggctcgatg
    13801 gcctgcatcc tcccgcggcg ggcgtccggg tgtcgttcgc cggaggcaag ccgaacgtga
    13861 ccgacggtcc gttcgccgag accaaggaag ttattggcgg ctattggatg atccgggtca
    13921 aatccaaaca ggaggccatc gcatgggcct cgcgctgtcc ggcgtcggaa aatgagacga
    13981 tcgaaatccg tcaagtgttc gagcctgccg atttcggacc cgagatcggc aaggaaggga
    14041 cagcgctgat ggaaaagatc aatcggaacc tggacgggaa tcggggcgca aaatcctgaa
    14101 atccagtgtg cgcaaaaaaa accaacttcg ctgtcgatat cgtcacgctc cattcgccgt
    14161 gacaatgcag aggacaaccg aaagaacatc gccatgcggt tcatgatgat tgtgaaagcc
    14221 accagggatt cggaggccgg cgtgatgccg aaggaggcgc tgatggccgc gatggccagc
    14281 tatcacgagg atctggcgaa agccggcgtg ctgctcgacg cttcggggct acagtccagc
    14341 aagaagggct ggcgcatcca attcgccaac ggcaagcgca ccgtggtcga cggtccgttt
    14401 gccgagacca aggagcttat cgccggttac actattcttc aggtgaagac gcgcgaagaa
    14461 gcgctcgaat gggccaagcg gatgccgaac cccacgggcg acaaggacgc ggagatcgag
    14521 gttcgccagc tattcgagct ggaggatttc gcacccggtg aagccatcga acgtcttcgt
    14581 gagttggaaa ccggattgaa gaaatagctg ccggatacag cgaaccccgg cagcaacacg
    14641 ccagcaaaca cgtaaggaga cccccaatgt acgtacagcc ctatctgttc tttgacggaa
    14701 aatgcgaaga agcgttgaat ttctacaaag cggcgctcgg cgccgaagtg agggcgatga
    14761 tgcgcttcag cgaaagcccg cagcccgctc cgccgggcat gattccgccc ggctccgaga
    14821 acaaggtgat gcatgcgtct ttccgcatcg gagatacgat cctgatggcg tcggacggca
    14881 acgccaaggg aaacccggtc tttcaggggg tgtcgctgtc ggtcaccgtg acaaacgatg
    14941 cggacgccca acgggtcttt ggggcgctct ctgcgggcgg tcatgtccag atgcctttgg
    15001 cgaagacgtt cttcgcctcg agtttcggca tggtcgcgga ccgcttcggt gtgtcatgga
    15061 tggttatcgc cgggcagccc tagagcgtga ttgatttgga ttgaatcgaa aatcacgctc
    15121 taggttttgt ttggtcgcat ttttccaggc tgaaccggca tccacttcag ccaaaaatgc
    15181 tctagtgcgc catcgggcga aaaggtttgc cgggcgtctg catgtcgtga aaggaagaag
    15241 cttatggcgt atatgctgtt gatcctggaa gggcatgacg accgcaagaa gcgcgggcgc
    15301 gatgagggcc gcgtggccta tgacagcatg atgcggtttg ccggcgacct cagcgcgcgg
    15361 ggccttctca agggcgccaa cgccttgagg tccgatgcgc aaggcgtgcg cgtcgcggtt
    15421 cgcgatggca ggcaaagcat cgtcgatggc ccgttcgcgg aatccaagga aatggtcggc
    15481 ggtttcttcc tgctcgactg cgcgacccgg gaggaagcca tcgccatcgc gcaagcgtgc
    15541 ccggcggcga aatgggcgac tgtcgaggtg cgcgaggtg
//
LOCUS       DASYEG010000006        43976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4495782, whole genome shotgun sequence.
ACCESSION   DASYEG010000006 DASYEG010000000
VERSION     DASYEG010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 43976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43976
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4495782"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..444
                     /locus_tag="VGM72_00225"
     CDS             154..444
                     /locus_tag="VGM72_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263714.1"
                     /translation="MGRHAWLLVGSGLDLVVLLAGFYMAISAVDVVSRSDRSPYAIGV
                     GLLFLALPVLAILAPLSAWRAAKRGRPGSRIATLFAAPWVYGAFLIVFLKYA"
     gene            650..1441
                     /gene="rpsB"
                     /locus_tag="VGM72_00230"
     CDS             650..1441
                     /gene="rpsB"
                     /locus_tag="VGM72_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY4263715.1"
                     /translation="MALPEFNLRQLLESGAHFGHRTQRWNPKMGSYIFGARNDIHIMD
                     LTQTVPLLHQALLAMRDVVAGGGRVLFVGTKRQASEPVAAAAKRCAQYFVNHRWLGGT
                     LTNWQTISHSIKRLRNVEETLTSAHTSGLTKKELLQIMRERDKLERSLGGIKDMGGLP
                     SMLFVIDTNKESIAVAEARKLDIPVAAILDSNSDPDGIAYPVPGNDDASRAIALYCDL
                     AARAVIDGLSAGQVAGGADLGEAEHVIEDLSTRSAPAEVEASPLG"
     gene            1492..2418
                     /gene="tsf"
                     /locus_tag="VGM72_00235"
     CDS             1492..2418
                     /gene="tsf"
                     /locus_tag="VGM72_00235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEY4263716.1"
                     /translation="MTTNISAAMVKELRDKTGAGMMDCKAALAETNGDVEAAVDWLRK
                     KGLAKAAKKAGRIAADGLIGVAIQAGQGAIVEVNSETDFVARGAAFQNLVRQAAQQAL
                     KAEGDLQRLLVSPVEGGASMADVLSALVGQVGEHMSVRRASALAVAPGVVASYVHNAA
                     APDLGKIGVLVAVESSGDAGKLMALGKQIAMHVAAANPLALSVDALAPDVVQRERAIY
                     ADQARQSGKAENVIEKMIEGRLRKFYEESVLLSQVFVIDGETPVAKVVENVAKDLGAP
                     VKIAGFLRFAVGEGIEKPTTDFAAEVKATAGL"
     gene            2460..3185
                     /gene="pyrH"
                     /locus_tag="VGM72_00240"
     CDS             2460..3185
                     /gene="pyrH"
                     /locus_tag="VGM72_00240"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEY4263717.1"
                     /translation="MTASLKYRRVLLKLSGEALMGAQAYGIDVSSVERIAADIKAAAA
                     AGAQLCLVIGGGNIFRGLSGAAAGIDRSTADYMGMLATIMNALAMQAALERVGLPTRV
                     QSAIPMMSICEPYIRRRAIRHMEKGRVVIFAAGTGNPFFTTDTAAALRAAEMGCDAMF
                     KATQVDGVYSDDPKRVKNAERYDFLTYHEVLSRDLKVMDASAISLSRENEIPIVVFSI
                     HEQGTLSAILKGQGRCTLIGERR"
     gene            3237..3803
                     /gene="frr"
                     /locus_tag="VGM72_00245"
     CDS             3237..3803
                     /gene="frr"
                     /locus_tag="VGM72_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEY4263718.1"
                     /translation="MAQLPPFDKEDMRRRMKGAVATAKHEFAGLRTGRANPAILDPVT
                     VEAYGSAMPLTQVGTVNVPEPRLLTVQVWDKSLAKAVDKAIRDAGLGLNPQLDGQLVR
                     IPIPELNEERRRELAKLAHKYAEAARVAVRNVRRDGMDHLKRLEKEHAIGEDQHRQSS
                     DELQKMTDENVHEIDAALTAKEQEIMHV"
     gene            3855..4685
                     /locus_tag="VGM72_00250"
     CDS             3855..4685
                     /locus_tag="VGM72_00250"
                     /inference="COORDINATES: protein motif:HMM:NF011408.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEY4263719.1"
                     /translation="MIKTILKSAVPESPSPSRLPKHVAIIMDGNGRWAKNRLLPREAG
                     HVKGISSVRKVVRAAREMGLQYLTLYTFSTENWRRPESEVRHLMGLFRRYCRADIEKL
                     HKHNVCVRFIGARAGLDPDIAELIEEAETLTARNTTLNLAFAFNYGSREEMAESARVL
                     ARAAAAGTLDPERITPAMFGQSMQSAGIPDADLVIRSGGERRLSNFLLWQAAYAEFVF
                     TDKLWPDFGAGDLEAALAEFARRERRFGGVAAMERGHREASLEEAGLRSLAAEVFDGE
                     "
     gene            4705..5550
                     /locus_tag="VGM72_00255"
     CDS             4705..5550
                     /locus_tag="VGM72_00255"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY4263720.1"
                     /translation="MTDTQPSMPARFELPARAASGVVMGVVAIAAVLGGGIFFACFVA
                     LGAIAALREWHRLVNGGHVAREMIPTGLAVICAVWLARAQTGLSWPFAAILLGAAGAA
                     ILSGTRGLPPYWPIPWHAVGALYVGLPALALVLLREAPRGAAIVGALFVAVWMADTGA
                     LLCGRVIGGPKLAPVLSPNKTWAGFLGGTIAAAIGEALYVAILGGNVPDAVALGIFLA
                     LAGHCGDLFESWVKRQFRAKNTGRLIPGHGGILDRIDSVLFAAPLWAGLIFLAGFNPF
                     NGAGP"
     gene            5547..6785
                     /gene="dxr"
                     /locus_tag="VGM72_00260"
     CDS             5547..6785
                     /gene="dxr"
                     /locus_tag="VGM72_00260"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY4263721.1"
                     /translation="MKPLVNHAPPDLDLSPVLGRRITVLGSTGSVGTATLDVVRFVRA
                     RYGAEAFPLQALTAQRNVALLAAQARELRPRVAVIGDVSLKDALADALAGTGIRALAG
                     PEAILDAAREPSDMVMAAIVGAAGLAPTLECVKRGAIVALANKECVVAAGEVFRRAAE
                     ESHGVLIPVDSEHNAAFQLLDFAQAHAIEKVTLTASGGPFRTWSLDRMAGATPEQAVS
                     HPNWSMGAKISVDSATLMNKGLELIEAHILFGLPPETLDVVVHAQSIIHCLVAYSDGS
                     VLAHLSAPDMRTPIAHALGWPRRIASPSRRLDFMEFTQLTFEKPDLSRFPSLQLARVC
                     LQTGGPAPTILNAANEVAVEQFLGRRIGFLGIARTVEQTLRTFAREMNGRAPETLDEV
                     LALDSAARARAREASGVAAA"
     gene            6819..7973
                     /gene="rseP"
                     /locus_tag="VGM72_00265"
     CDS             6819..7973
                     /gene="rseP"
                     /locus_tag="VGM72_00265"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEY4263722.1"
                     /translation="MPAVVADFVSWMGVGLPAFLFVITLVVFVHELGHFLVARWCGVT
                     VETFSIGFGREIVGFNDSKGTRWKLSWIPLGGYVRFFGDADASSRPDREKLKSIGALD
                     GIGKRVEFVKDPQSGVLHFKPLYQRALVAAAGPIANFILAIVIFASLILTVGKPAEPP
                     IVEAVMAGSAAEMAGMQSGDTIRSVDGQAIRAFIDLQRTVGANAGKTLRVVVNRGGRD
                     VALDVVPTATEITDASGAKHSQGRLGIRDATVSVGIVEAIGGGLDQTWFIVSQTGSYL
                     GKIVTGRESPDQLHGILGIGAVSDQAARIGFAALIGLAGLMSVSIGLVNLLPIPVLDG
                     GHLLYYAFEAVLGRPLGERAQEVGFRLGLAFVLCLMLLATFNDLVRLNLF"
     gene            8155..10440
                     /gene="bamA"
                     /locus_tag="VGM72_00270"
     CDS             8155..10440
                     /gene="bamA"
                     /locus_tag="VGM72_00270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEY4263723.1"
                     /translation="MPVPPGPQMTLDQAAPVMMPMPAPPGQGMVQRIVVEGTQRIEPD
                     SVLSYLLLRQGQAYDPAVADRSLKVLFDTGLFSDVRMDFDGTTLTVRVVENPILNQVV
                     YEGNSNTSDADLNKEVQVRPRMVFTRARVQADVQRIIELYRRAGRFAATVEPKIIQRP
                     QNRVDLVFEINEGPTTGVAAINFIGNRVFPDTDLREQIATTTSAWWKFLTTNDNYDPD
                     RLTFDREQLRRFYLGRGYADFRVVSAIAELAPDRKNFYVTFTVDEGELYHFGKIQINS
                     RIRDLNPADLQPLLLTNEGDVYDAGLVDKSIEALTFAAGTRGFVFIDIRPRVQRNPMT
                     KTVDLFYEIDEAPRVYIERINIVGNTRTRDEVIRREFRLTEGDAFNRVLADRSRTRIR
                     ALGFFRDVTITEDPGSAPDRTVLTVNVTEQPTGELSLGAGYSSQQGLLLDFSYTERNL
                     FGRGQFLRASVSIGTFQKDYDFRFTEPYFLGRPLAAGFLVYKVITDFTDQIGYISDVT
                     AAGIQFGFPVSEFGRISPHYNFSYTRIDSTVTASEAIQLFPAYSASSVGYTYTYDTLD
                     DAIKPTKGWNFALSQDVSGLGGELKYLRSVAAVEYYHPFFFDLVGEFALNAGYISGYG
                     GLAVPINERFFKGGPSFRGFEIAGIGPRDLVSDSALGGQFYLIGTYQVRLPDILPPDY
                     GINLSAFSDFGTLGLDRSRALECASAASCKDDLAPRVSLGIAINWKSPFGPVEVDVGY
                     PIIKQSYDKPQAIRLSAGTQF"
     gene            10463..11224
                     /locus_tag="VGM72_00275"
     CDS             10463..11224
                     /locus_tag="VGM72_00275"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEY4263724.1"
                     /translation="MSKTHHCRTAAAIAALTLGFLISGSQEGPSGAQAAPAPAATAPG
                     VVVARPATAPVSTAPAPAAPSVAAVAGAGTVPRIVVIDRNFILQRSSAGQDMISQSQN
                     LSKQAETQFKAEEAALQTEMGQLQQQLAILAPDVRDQKEKDFTAKQQAFQAKVQQRQA
                     EIQAGFNKAARSLEVALEPILKTIMMERGANMVLDRTSVILSSVDVDVTPVAVQRLDK
                     ALPHVKVELTAVPATAAARPAGVPALTPAAAAVKK"
     gene            11259..12290
                     /gene="lpxD"
                     /locus_tag="VGM72_00280"
     CDS             11259..12290
                     /gene="lpxD"
                     /locus_tag="VGM72_00280"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEY4263725.1"
                     /translation="MADPRFYDNRGPFHLAELCVSARLAPPADSNADAPIYDVAGLEE
                     AGPPHLTFYDGRRNKAGFLATKAGWCLADNRLIETAPASTVVIPVPSVARAFAAMAAR
                     FYPEHELDIRRLESAVHPSAKLGQDVVLAPGVLIGPDAEIGDGARIGAYAVIGRGVTI
                     GRSAEIGPHASIMFAHLGDDVLIQAGAKIGGSGFGYSSGPDGHIKMPHLGRVIVQDGV
                     EIGANSTVDRGMLSDTVIGEGTKIDNLVQIGHNTVTGRHCVLAGQAGTSGSVVLGDFV
                     IVGGGVGISDHLTIGDRARLAGHSGVAHDLEGGRDYGGIPARPIRQWHKEGLLIAKLA
                     RKGVRFTDG"
     gene            12328..12798
                     /gene="fabZ"
                     /locus_tag="VGM72_00285"
     CDS             12328..12798
                     /gene="fabZ"
                     /locus_tag="VGM72_00285"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEY4263726.1"
                     /translation="MEQLKRLLPHRPPFLMIDRLSDIKAGASAVGWKAVSINEPHFVG
                     HFPEYAVMPGVLIVEAMAQAAAAFVVHTLGLQDQRRMVYFMTIDKARFRRPVHPGVVL
                     RMPVQVQRRRGPVWRFEGKAFVGEELCAEAEYSAMLTEHSLGMVGSPVLSGGAE"
     gene            12798..13610
                     /gene="lpxA"
                     /locus_tag="VGM72_00290"
     CDS             12798..13610
                     /gene="lpxA"
                     /locus_tag="VGM72_00290"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEY4263727.1"
                     /translation="MSKIHPTAIVEDGAALGSDVEIGPFSLIGGHTRLADGVRILSHV
                     VIAGHTEIGRASVVHPHAVLGGGPQFRGDTGAEARLRIGAENIIREHVTMNGGSVKGG
                     GLTQVGDRGYFMAYSHVAHDCHIGNGVTFANSVALGGHVTIEDGVNIGGLSAIQQFSR
                     IGRYAFVGGVTGVPDDVIPYGMAWGDHARLEGLNLIGLKRRGIPRERIHALRAAFRAI
                     FFGSGRLFERARDAGQRWKDFPEVSEVVAFILADSKRPICMPSATHAGAETG"
     gene            13616..14476
                     /gene="lpxI"
                     /locus_tag="VGM72_00295"
     CDS             13616..14476
                     /gene="lpxI"
                     /locus_tag="VGM72_00295"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HEY4263728.1"
                     /translation="MDAPGGTLGIIAGGGDLPIVIARAAQADGRAVFVLALAGMANPS
                     DVAAFPHGFASLGELGKAIRLLKDSGCSEVTFAGKVARPEFSKLKLDARGALALPRVM
                     AAALKGDDALLRSLVAIFEKEGFRVIGSTDAACGLVAPLGTLGKFAPARDSDGDILHA
                     IRVVAALGAFDIGQAAVVCEGLVLAVEAAEGTDAMLARVAALPEALRGTPEHRKGVLV
                     KAVKPNQERRVDLPVIGVRTVELAAAAGLSGIAIEGAAALIVDRARVTQTADRFGIFI
                     YGFAPEDYPR"
     gene            14473..15657
                     /gene="lpxB"
                     /locus_tag="VGM72_00300"
     CDS             14473..15657
                     /gene="lpxB"
                     /locus_tag="VGM72_00300"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEY4263729.1"
                     /translation="MSADAESRTLTIMLVVGEPSGDKLGAQLMAALKQVAGKHVRIVG
                     VGGAAMAAEGLESLYPLDATSVMGLREVVPRIPAILRRVREASDFAILTRPDLVVLID
                     SPDFTHRIAQRLKRIDPSIRTANYPPPQVWASRAYRARKMARYMDAVLTMFAFEAAFF
                     ERHGIRAYNVGYPVIERADKIAGGAEFRRRHGISADAPLLVVLPGSRRNEIRFILPPF
                     RAAVEMLARDIPGLVTVLPTVSHVAALVKAAVAKWPTPLHIVDTEPEKYAAFDAADAA
                     LAASGTVTSELALAKVPMVVAYKVGWLTYALAKPFMHVRYMVLVNLVLGRKAIPEFEQ
                     YAATPQALVQALKPLLTDESARANQMRDLEEGVRAFGMGQETPSLRAARVVLELAGAK
                     SS"
     gene            complement(15664..16428)
                     /locus_tag="VGM72_00305"
     CDS             complement(15664..16428)
                     /locus_tag="VGM72_00305"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY4263730.1"
                     /translation="MNPINSIARSFLAPLTILTLMGSAASAAEIQGQKIMLPAKERQV
                     HVTYFRAPGNGPRPSALLLHGAGGFDRQIANNNRYAAALAAQGIDAYLVYYYSDMDDK
                     MMSNGVNVFEDRYPQWAKLVDDLADYLIAQKDSNGKVGLVGFSNGGILATGASTLDPK
                     INAAVIYYGTDPWPLGQPAKRFPPLLVLHGDADQIIPVENGKQLADEAKKLGAKVDLV
                     IYPGESHGFGARWDDKNATDALNRAVVFLKRELQVK"
     gene            complement(16425..17201)
                     /locus_tag="VGM72_00310"
     CDS             complement(16425..17201)
                     /locus_tag="VGM72_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY4263731.1"
                     /translation="MNPIRHFAVALALLVSSGFAQSVSAGEIQGKEMTLPATERAVKV
                     EFFKAPGTGKRPTALMLHGGAGWGGPDGRIENFRHYGSELANHGFDAYMVYYYSDEDA
                     KIMADNKTRGGYTQKRFPAWAKLVSDLTADVKKMPGSNGKVGLVGFSNGGNLSAHATP
                     LDTNIDAAVIHYGGVSRVPGYAPARFPPLLVMHGEADTRQPMALGQRLVDQAKAVGAS
                     VEWVTFPGKNHGFAADLNHPAAAEAFKHTIAFLDKQLKGE"
     gene            complement(17206..17997)
                     /locus_tag="VGM72_00315"
     CDS             complement(17206..17997)
                     /locus_tag="VGM72_00315"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY4263732.1"
                     /translation="MARNLVAPLALLAVSVLAPAANAADIKGNANAADIQGKEMVLPA
                     SDHPVKVEFYRAPGNAKRPTVLMLHGGQGWGGAEGRTENFQHYASELANHGFDAYMVS
                     YYSDQDVIDRKANAPGISVKRFPAWAQEVSDLTADVKKMTDSNGKVGLVGVSNGGNLS
                     AHATPLDKNIDAAVVYYGGVSRVSGYEAKRFPPMMIFHGEADTREPIERGRKLYETVK
                     ALGGPVEFHSYPGINHGFGQNLGTPAADDAFQRTLAFLDKTLKGK"
     gene            complement(18243..19559)
                     /gene="gltA"
                     /locus_tag="VGM72_00320"
     CDS             complement(18243..19559)
                     /gene="gltA"
                     /locus_tag="VGM72_00320"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEY4263733.1"
                     /translation="MNIKASGAKPNGTATLNYGNKKVDLPVYKGTEGPDVVDIRKLYG
                     EADLFTYDPGFTSTASCESDITFIDGDRGILLYRGYPIDQLAKNSNFLEVCYLLLNGE
                     LPTPKQSSDFKNTITYHTMVHEQISFLFRGFRRDAHPMAVMVGVVGALSAFYHDSTDI
                     TDKRQREIASHRLIAKMPTIAAMAFKYSIGQPFVYPLNHLGYTENFMRMCFAVPCEEY
                     KVNPVLTKALDTIFVLHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGPAHGGA
                     NEAALKMLQEIGTIDRVPEYVKRAKDKSDPFRLSGFGHRVYKNYDPRAKVMRDQCYAV
                     LDELGKHDDPLLKTALELERVALQDDYFIEKKLYPNIDFYSGITLKALGFPNDMFTAL
                     FALARTVGWVAQWQEMIEDPEQKIGRPRQIYTGPHRRDYIPPEKRG"
     gene            complement(19675..21087)
                     /gene="gltX"
                     /locus_tag="VGM72_00325"
     CDS             complement(19675..21087)
                     /gene="gltX"
                     /locus_tag="VGM72_00325"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEY4263734.1"
                     /translation="MAENSVPVLRFAPSPTGYLHIGGARTALFNWLYARHSGGTFLLR
                     IEDTDRERSTPEAVAAIFNGLSWMGLDWDGEPTFQFARVSRHREVAEQMLAKGQAYRC
                     YATPQELEQMRAEQKAKGLSIRYDGRWRDRTPGPEQHNMPYVVRLKAPQTGETIVHDV
                     VQGSVRWANDQLDDMVLLRSDGTPTYMLAVVVDDFDMGVTHVIRGVDHLNNAARQLQI
                     IQAMGWPVPVYGHVPLIHGSDGAKLSKRHGALAVEAYRDMGYLPEAMRNYLLRLGWSH
                     GDDEIIPTEKAIELFDLAGIGRSAARFDLKKLESINAHYMREMSDGALAGETLAFAQR
                     IGPGRSFDAILRARLAAAMPFLKPRAKTLVELLEKGDFLFISGTPAIDKAAAAALTPE
                     TRGWLRRLALELEGADWNTSAIEACTRGFAEREGIKLGDIAQPLRAALTGRTASPPVF
                     DVLAVLGREEALTRIRAQGD"
     gene            21133..22668
                     /locus_tag="VGM72_00330"
     CDS             21133..22668
                     /locus_tag="VGM72_00330"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEY4263735.1"
                     /translation="MRIGASGIGLTRRWRAFAALTWPQQASANAVSFPGIAGAAEPRF
                     HARALQVLANAAERNLAAEQERWLAWWVAGFAAGILIYFALQTEPSLPMTALVVVTAS
                     LCAWSRPRNAGTAMRFFCAMIAAGGLGFSAAKLRTLRIDAPIIAHDMGPVRLTGRIES
                     VDIRSDKRARIVLAPATLGDGKTARPERVRLTLMGAKAVAAVQPGAVVSALAVLRPPP
                     EPAMPHGYDFARWAYFNAIGGVGFTYRAPKLLDAPPPGLLDLARARVENLRLAMTRRI
                     TAAIPGADGNIAAALITGERGEIDEDDNQAYRDSGLAHVLSISGVHLALAGLGIFWAL
                     RALLALWPWLALTQPIKKWAAIAAFASSSFYLAISGGGSPAVRSYLMLSMMLLGVLAD
                     RPALSMRAVALAALALLAYEPEDIVDPSFQMSFAAVIGLIALAEWGASVVRNDTPAPG
                     RGAHFWRKGRRYVFGMLLASAVATLASTPFAIYHFDRAAAYSLLATIIDFPEVPGKSR
                     KVP"
     gene            22782..24068
                     /locus_tag="VGM72_00335"
     CDS             22782..24068
                     /locus_tag="VGM72_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY4263736.1"
                     /translation="MITESQIKSALRAAPTCGKKSIELKDGGSRGEGRLALIVRAGES
                     RITTEWYAVYYRGGKRTTTKMGSYPTYGIADARKLFREEYAPKISSGENPTGPRSRAN
                     KIGVTVLDLFQAYVAHLKANASPRSAVTARFILLGPPSRPGGAARAIGEDMRAADVRP
                     ADITPHLSEIHARGCVALAADTRTYISAAFAFVIKSANSYTQPAGATKWGIETNPVAA
                     IPADPAGARIGDRHLSPSEFRAFWRWLEREDSNSAVAPALRLIMATGQRVTEILELTN
                     TSYDRGERMLDWRKTKNGRQHSIPLPTQALSILDGLVVNRHGLFFPHGRRPDEPATIY
                     SPPKVIYHYAEDTGAAPFTARDFRRTWKTLTGAAGLSKDIRDRLQNHARQDVSSRHYD
                     RYEYLQEKRAAMATWSAYLDRILAGEFDKNAMKEAA"
     gene            24776..25897
                     /locus_tag="VGM72_00340"
     CDS             24776..25897
                     /locus_tag="VGM72_00340"
                     /inference="COORDINATES: protein motif:HMM:NF024755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HEY4263737.1"
                     /translation="MRHKKTPPPARETALSICLPRHESIIKGYCHTAAAQVRVPDVAR
                     AVRVGGGAMSGANTEIAAVRDRVDLAALIGRRVKLARKGRASVGLCPFHSEKSASFKV
                     ENGRFICFGCGARGDCIDFLMQADGLSFREALRALSDQVVAIPPRAAARSSPEAWPKD
                     DAKRIAGAIRIWNESGALRGTPGWRYLQSRGIDTDALSDRLNEVLRWHPRCPWKDGTH
                     GCVIALWTDALMGEPRAIHRTGITAGGEKIGRLALGPKKNCVIRLWPEVTQSLVAGEG
                     IETVLAAATRLTHRGCPLRPAWAVGDAGGLAALPVLPGIESLTLLVDHDINEVGQRAA
                     ATCSSRWTAAGIEVRRLLPKAPDSDFNDVVVLNGAWHEG"
     gene            25887..27533
                     /locus_tag="VGM72_00345"
     CDS             25887..27533
                     /locus_tag="VGM72_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263738.1"
                     /translation="MRDNRDALAEALGMPPGPEDKLRLPAFAVPKIGGPQAREYLQRL
                     VHKSLDLIVEHGEAKAWTEGRTEELLSASADPDGERAKILSRAKREAKRRFTAEVLKP
                     LRLQIAASAGVGKTTAVANGYRNKPALWRKSIWYFAPTVDLCEQVVRDINENAPRGMP
                     LARVLAGRTHSKNGRVPPCRRAKIVQESQIKVRSVYKAFCDDGAGQRCAFYEQCDYIA
                     ARRDQRPGILAFPHANLVVPQSNDLKLPAPDLVIIDERCFDKFTKKSIIRAEELQDRD
                     TYFRIGAPAEENAGYERHAELGRYIAKIIIEEKDWLHRLRADEDKARLDSGVGNDWFL
                     IGNPKSLHNRLSEAASAAAAAAGSNIQIVPTDDDGAVREKLSAIKSHSGRRVAGLFRR
                     IRRDLESPAESVAVLTEVSNGETVIVCHGLRELAFSESTPLLIIDADASERINNRVFS
                     RSDRSEKLRHVDVPVRRHAKFIQCDSSVFSKSGFHGKGEEKSNSIGTRGKEQIDRIAA
                     FAKKVAGDHKRLLMSANKEIRCAGTGEPCGAWFFRTLPAR"
     gene            27487..27789
                     /locus_tag="VGM72_00350"
     CDS             27487..27789
                     /locus_tag="VGM72_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263739.1"
                     /translation="MSLAAPGFFELCPRVEITHFGAFTGQNRWSRFDVGIIIGREQPP
                     PESIECLARALYADAPRPTLNFGGEYVTATRQYEPGGTRWADFDAHPDPAVNEIWR"
     gene            27780..28418
                     /locus_tag="VGM72_00355"
     CDS             27780..28418
                     /locus_tag="VGM72_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263740.1"
                     /translation="MEIVRERQTLQAIDRLRLLCPDGRDPEVYILCKIPLPGISPDRL
                     ESAKEIFAGGKKIDQALAGGIFSTNPNILAHCHPDLWDTPKAAERWLESHKSETPNLH
                     IDTLLIAKWGFLNRLEFRVKKPGSRRWSIVEIDVAKYPDPEKIVRAALRVPAAVEIEF
                     RGPAWDEIRKRAENPVAIQTARKPKLLVFIRAAEDAELLRRIRGRPPTQFAA"
     gene            28667..29287
                     /locus_tag="VGM72_00360"
     CDS             28667..29287
                     /locus_tag="VGM72_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263741.1"
                     /translation="MAATKNAYDALVEEIRNGYFDDGEPVMKSLRVITSDGSRVEALD
                     ASEHVAELLARISDGEEALVKALAPIVDLLKSTTGKVAKIAKARRAAAPSMPADEFFS
                     RALAAQAAGKVSGLDIAKAEACLARGLRLPDEIVEKVLGDETYKALPAEMSYQAFMDR
                     AEAALAAGKVTHLEFSRAQAALMNGRQPAPAFLSRLLFGHSGTLAA"
     gene            29708..30082
                     /locus_tag="VGM72_00365"
     CDS             29708..30082
                     /locus_tag="VGM72_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263742.1"
                     /translation="MSDYDAWAGRLRQPHFDWADATQELKSDDIEQLTESFLDSATFN
                     AVRTRVREGFVPSRQLDWGSWAVPATKDDILAHMARWQPGWDLPEPGDETHKPRLQRA
                     RCLQAVEALPADGEYVLVIQES"
     gene            complement(30134..30811)
                     /locus_tag="VGM72_00370"
     CDS             complement(30134..30811)
                     /locus_tag="VGM72_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEY4263743.1"
                     /translation="MQAQSSKFVAYLRVSTAKQGRSGLGLEAQREAVRQFVVGRGGKI
                     IAPEFVEVETGKDDARPELDKALKRCRITGATLVIAKLDRLSRNVAFLMRLRDEGAKF
                     VAADLPDANTLTLGVMASMAQHEREVISRRTKDALAAAKARGTVLGGYRENAPDIARF
                     QRKGAQAARKAADVAAERYRETIDAMQAENLSLNAMAARMNETGIMTRTGKQWTATAV
                     RNLVARL"
     gene            complement(31082..31939)
                     /locus_tag="VGM72_00375"
     CDS             complement(31082..31939)
                     /locus_tag="VGM72_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263744.1"
                     /translation="MVAYFRKSACLVRTPVFSRSHAMKWTSLPVLAVSIMLAVSAHAE
                     LSETELSHVGLSLPNEARLPLSLSFRDDAGRSVSLAEALQGLPALVIPVDYTCKSTCG
                     TALTLTSDALADSGLKPNSDFRLIVIGIDSNDRPDDARRMAIGQIHRQEITAASAVLL
                     GDAPTTRMFLRDIGYRAVYDAAADQFAHPAIVLAIARDGRIVRGLSSLALDPTELRLA
                     LIDAGEGRIGTIGDRIRSICYGFDAAHGIYTPLVQRLLIGTGLVTIFLFAMFFAVAFN
                     LPRRLLRRN"
     gene            complement(31870..32919)
                     /locus_tag="VGM72_00380"
     CDS             complement(31870..32919)
                     /locus_tag="VGM72_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263745.1"
                     /translation="MTPRAILLAIAVTGLALCGVGLFVDTKAALSAYLAACVTVASIP
                     MGALAVLLTTYLVRAAWTEGMHAPLAAASLTIPGAGLLFIPVFVGLSWLYPWATRVEP
                     GPLKVIYLTPWFYILRTLIYLASLSAIAFWAARAWKKLAEMIRVASIGLIVYALVASF
                     AGIDWIESLEPSFHSSEFGLIFLASQLLAGLCFALVMTLWHQKPESTHAYGATLLSTL
                     LLWAYLQAMQYIIIWSANIPDEALWYLRRAEAPWDAVLWTLAILQFVVPFFALLSMRV
                     RTGRTPILTIACVTLALRYLEAIWLIFPATGTSNWILWIVIPAAICGVGGTWLLIFER
                     ALAWSAHQFSREATP"
     gene            complement(32916..33455)
                     /locus_tag="VGM72_00385"
     CDS             complement(32916..33455)
                     /locus_tag="VGM72_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY4263746.1"
                     /translation="MIRFLIPVILLIPLCGCNQDMISQPKDNEYKPAELFPNNRILQR
                     PPLGSVARGDAARTKQAAEKPIPTLALLERGQHEFDVFCSPCHGRLGDGRGMIVARGF
                     PAPPTYHQERLRAASDQHFYDVISNGYGVMYSYADRVMPADRWAIVAYIRALQLSENA
                     SLDDAPPAERGKLVTEAQP"
     gene            complement(33452..33988)
                     /locus_tag="VGM72_00390"
     CDS             complement(33452..33988)
                     /locus_tag="VGM72_00390"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEY4263747.1"
                     /translation="MPDNSALYGIIGAFKSPEELVAATRLAKQEGYCHLDAFTPFPVE
                     ELNGILELNDNRVFWLGLTGGFFGAGVALAMQVYVNFDYPLNVGGRAQYAWSAFAVVA
                     FELTVLFAALFPALGMLALNGLPKLYHPVFNAPSFGRASSDRFFLCIGAHDPSFDSAK
                     TAEFMRNLKADAVELVPE"
     gene            complement(33978..35348)
                     /gene="nrfD"
                     /locus_tag="VGM72_00395"
     CDS             complement(33978..35348)
                     /gene="nrfD"
                     /locus_tag="VGM72_00395"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEY4263748.1"
                     /translation="MSFASDAARPVLPPDQDLGDVTDQIARIPLHFPTRSRWLIALCL
                     GFALFTLFLVSATVLFVRGVGVWGLNIPVNWAFAIHNYVWWLGIGHAGTLISALLLLM
                     GRDWRNSLNRFAETMTLFAVVCAGLYPVLHLGRPWFLYWMFPYPSTMGVWPQFRSPLE
                     WDIWAVLTYLTVSVIFWYIGLIPDLGAARDRARKRGWQVFFGVTSLGWRGSAIHWMRW
                     RQCYRLIAALAVPLVVSVHSEVSLLFASSQIPGWHSTIFPPYFVLGAAFSGFAMVAII
                     AIAIRALFNLQNLVTDDHLDVLGKVLLATGFMTAYGYVFEIFDALYSSNVHDLQTLQD
                     RFSGTYAWSYWGAVFFNFIVLQGLWWRSVRRTPWTFFTIATSVVIGMWLERYMILVTS
                     LYRDFLVSSWGLYTASFWDWATYFGTIGVFLVPFLLIIRVFPVISIFETKEVLHEEHK
                     EGSHAG"
     gene            complement(35348..38251)
                     /locus_tag="VGM72_00400"
     CDS             complement(35348..38251)
                     /locus_tag="VGM72_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HEY4263749.1"
                     /translation="MKTARTPSLQLEKIRQKLHGTRGRTFWAGLEEVADEPDFREWLT
                     AEFPAAAEVPQNGRREFLRLMAASFALAGLAGCSERSRADLAAPFVEQPEYLVPGVPR
                     YYATATMFGGYAQPAIATSYDGRPTKLDGNAQHPIGSSASDVFTQAAILGLYDPDRSN
                     IPLRDGVPATWADFQQAIASFRDHWKSQGGKGVHVLMGHTTSATLIRQWTQFLAEFPQ
                     AALYLFEPASDARGGPMHLHHDLSQCDVLVGIEDDVLGPGPQQVSNASAWSSRRGEER
                     PGAERMRMYMAESTPSLTGSVADRRIPVDASRLPDIAAALASAIGVTGPGTVQLSAKE
                     QAWVVAAARDLQQHAGRSLLTIGGQLPLEIQELAPLVNQKLGNAGATVRYSEPFIFMP
                     QHASTIATLVGAMKAGEVDTLIVIDSNPAYSAPGELAFADAMGRVNKRIHFGYHADET
                     GRLANWHLPLAHMLESWSDGRAVDGTVTLVQPVIEPFYNVISVHQALDMLLGVVDPDA
                     ATAVRLTWKDRLGDPLGPRWLDALDAGFISGTAVEMDTPFPAANTGTIVTQSLNNQLD
                     IVFRPDPSVWDGRFGNIGWLQELPKPLTKITWDNVVLVSPALAERVVLANGDNVEIAV
                     GNRTVIGPVWIMPGQASNTVTLFLGYGRKNGGHIAGDIGYNAYAVRPASNAWHAVGTL
                     RRAKGEHRLASTQLHHRMDGFDLVREVTAARPALAKPPAQPSFYPEWGGTDVAWAMVI
                     DLDLCIGCNACVTACTAENNVAIVGREQVAMGREMQWIRVDRYYTGDVEDPQSYFQPV
                     PCMHCEKAPCEMGCPVNATVHSDEGVNQQVYNRCIGTRTCSSYCPYKVRRFNWYDYTG
                     GDDPSTIAAHNPDVTVRARGVMEKCTYCIQRIQGAHATADKENRAIKNGEVVTACQQA
                     CPTKAIVFGNLNNADSEVAKRRQSGRHYALLEELGTRPRTTYLAQWKDEGEES"
     gene            complement(38248..38607)
                     /locus_tag="VGM72_00405"
     CDS             complement(38248..38607)
                     /locus_tag="VGM72_00405"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEY4263750.1"
                     /translation="MLAPVRESLSQDRSLVWQRVARLPDYVYFRHDVHIAKGVGCVSC
                     HGRIDEMALTYKAVPLTMQFCISCHRNPAPALRPANEITHMNWRPRGDLAALGSSLLV
                     QNHIRVGELTDCSVCHR"
     gene            39022..39390
                     /locus_tag="VGM72_00410"
     CDS             39022..39390
                     /locus_tag="VGM72_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263751.1"
                     /translation="MLQALTPVLKPAANEPTDISGLLMAYLAISLIGTVVGLSLLLRI
                     IFPSARDAPPISSLADLGGLLQLQIDEAGDFQKLRRAEDESLNSYGWINRNDKHIRIP
                     IRRAMELTAKRGIPGWPTRR"
     gene            39445..40389
                     /gene="coxB"
                     /locus_tag="VGM72_00415"
     CDS             39445..40389
                     /gene="coxB"
                     /locus_tag="VGM72_00415"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY4263752.1"
                     /translation="MQWIPFFTPGDSQYSNDVDLLFVGLLTASVLMLVLLFGLMLIFI
                     VRFRENSGVKRPHPAEKSWRWEIAWTSATALIFVIMFAWGASLYVRIYRPPDDALTIN
                     VVAKQWMWKAEHPGGQMEINALHVPVGRAIRLIMASQDVIHSFFVPAFRLKHDVVPGR
                     FQSLWFTALRPGTFQLFCAEYCGTDHSRMTGSIVVLRPAEYQDWLRDQVGGDTLAAEG
                     ASLFRSLGCSGCHAPGSTVHAPRLEGVYGNPVPLSDGTIVPADDRYIRDSILQPRSQI
                     AAGYEPIMPVFAGQISEDELLRLVAYIKSLANAKRGAQ"
     gene            40386..41996
                     /gene="ctaD"
                     /locus_tag="VGM72_00420"
     CDS             40386..41996
                     /gene="ctaD"
                     /locus_tag="VGM72_00420"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEY4263753.1"
                     /translation="MNAHAPVNYLTTDYRLSSWFTTTDHKRIAILYFATITLFFFIGG
                     MAATIIRLELATPAGDLVTSDVYNRLFTIHGIVMVWLFLVPSIPTTLGNFLVPLMIGA
                     RDLAFPKLNLLSWYLFVAGGACVLYALFVGGLDTGWTFYTPFSSTYSKGNVFVAVLGI
                     FIAGLSSILTGLNFIVTIHRLRAPGLTWFRLPLFIWAIYATAIILVLATPVLAMTLLL
                     MTLEREFGVPIFDPARGGDPLLFQHLFWFYSHPAVYIMILPGMGVVSELIPCFSRKPI
                     FGYKFVAWSAISIAIVGFLVWGHHMFVSGQSVFAGVVFSLMSYLVAVPSAIKVFNWTA
                     TLHGGWIKLDSPMLYALGFIGLFTIGGLSGLFLACLAYDVYVTDTYFVVAHFHYIMVG
                     ATVTAYFGGLHFWWPKITGRLYPEYWARAAAIVIFFGFNLTFFPQFILGVLGMPRRYH
                     VYPAEFQVWNVLSSGGAFVLAIGYLMPLIYLSWSIFWGKRASANPWAATGLEWQTLSP
                     PPKYNFAVTPTVVREPYCYTPEALPQNG"
     gene            41998..42633
                     /locus_tag="VGM72_00425"
     CDS             41998..42633
                     /locus_tag="VGM72_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY4263754.1"
                     /translation="MHPEMQFSDLGQQQEIARLGIWVFLATEILFFGGPLLLYTVYRG
                     AYAQGFAAAARHTDILIGTANTFILLTSSFLVAWATEAIKKDDGRLTALLFGAAVFLG
                     LVFLGLKGMEYSNEYHEQLIPGFGFAFAEAERNAALLFFTFYFITTILHAIHLMIGIV
                     LLIVIARHAAKGRYGAGYNAPVFVSALYWHFIDLVWIVLFALIYLPGRSGP"
     gene            42630..42971
                     /locus_tag="VGM72_00430"
     CDS             42630..42971
                     /locus_tag="VGM72_00430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEY4263755.1"
                     /translation="MTANSRSWKPPARLGIAWIGLVLLLGSTVSLSFVPLGWENTAIA
                     LFIAFAKVAIVAVIFMELDKGKALVRIFALAGLVWLAIMFWFVLNDYATRSEFPPPAA
                     ARPALSDSSDH"
     gene            43070..>43976
                     /locus_tag="VGM72_00435"
     CDS             43070..>43976
                     /locus_tag="VGM72_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY4263756.1"
                     /translation="MAAKDEGGHEVHSDGAAGGWGSLRGIGRVLLEEHDTPEVAATLM
                     RQNKPGGFMCVSCAWTKPADYHPFEFCENGAKATLWELTKRRCTPETLEKHTLAELRE
                     WKDHDLEQLGRLTHPMRYDGRSDTYVRCNWDDAFAAIGAELTALDPKSTVFYASGRAS
                     LETAFLYALFARLYGHNNLPDSSNMCHETTSVALKKVIGAGVGTVVLDDFEKCDAIFF
                     FGQNTGSNSPRFLHDLQAAVERGVKIITFNPVREKGLEVFINPQNLAQMLTGSETHIS
                     CQYHQVKAGGDIAALLGLCKHVLEAD"
BASE COUNT         8571 a        12891 c        13517 g         8997 t
ORIGIN      
        1 ggttgcggga aagggactgg tcacgctcat cgtgccgggt caatccggca atcaggatgt
       61 ggagatcgcg ctccccaaac gcgtttccgt caatccgcag ctcaaatcca cgctcgctgc
      121 gctgaccggc atcgccgaaa ttgagaccgt ttaatgggga ggcacgcctg gctcttggtt
      181 ggaagcgggc tcgatctggt ggtccttctg gcgggattct atatggctat ttcggcggtg
      241 gacgttgtca gccgcagcga ccgttcgccc tacgcgatcg gggttggact gttgttcctt
      301 gcccttccgg tcctggccat cctggcgccg ctctctgcct ggcgcgccgc gaagcggggg
      361 cgccctggca gccgtatcgc caccttgttc gcggcgccct gggtctacgg ggcgttcctg
      421 atcgtgtttc tgaaatacgc ctagacgtgg aacagcgcgg ggcggatttc tctttcccct
      481 atggaacttt ggtcaaagtc ttgcgccagg ccgaagaact acctataagc ccggccattc
      541 cacacgcggg gccgggcttt cagccgatca tggttggcag cccatccggt gttcctgacc
      601 tttaggaatc actcccgcgt taggctgaac cggaaaaaca ggagttacta tggcactgcc
      661 agaattcaac ttgcgccaac ttttagagtc gggcgcgcat ttcggtcatc gcacccaacg
      721 ctggaacccc aaaatggggt cctatatctt tggcgcgcgc aacgacatcc atatcatgga
      781 tctgacgcag accgtacccc tgctgcatca ggcactcctc gccatgcgcg atgtggtggc
      841 aggcggcggg cgcgtgcttt tcgtcggcac caagcgtcaa gcgtcggagc cggtcgccgc
      901 ggcggccaaa cgctgcgcgc aatattttgt caatcatcgc tggctcggcg gcacgctgac
      961 caattggcag acgatttcgc actcaatcaa gcggctgcgg aacgtggaag agacgctgac
     1021 ctcggcgcac acctccggcc tgaccaaaaa agaattgctg cagatcatgc gcgagcgcga
     1081 caagctggaa cgttcgctgg gcggcatcaa ggacatgggc ggacttccca gcatgctgtt
     1141 cgtgatcgac acgaacaagg aatccatcgc ggttgccgaa gcccgcaagc tggatattcc
     1201 ggtggcggca attctggatt ccaattcgga tcccgatggc atcgcctatc cggtaccggg
     1261 caatgacgat gcttcgcggg ccatcgcact ctactgcgat cttgcggccc gggccgtgat
     1321 cgacggtctc tcggccggtc aggtcgcggg gggggcggac cttggcgagg ccgaacatgt
     1381 gattgaagat ctgagcacgc gctctgctcc ggccgaagtc gaagcaagcc ctctgggctg
     1441 aatgtccgcg acctcgcgct taagccaatc aggggcgaag gaaatcctcc aatgacgacg
     1501 aacatttctg ccgcaatggt gaaggaactg cgcgacaaaa cgggcgcggg catgatggat
     1561 tgcaaggcgg cgcttgccga aaccaatggc gatgtcgaag ctgcggtcga ttggttgcgc
     1621 aagaaggggc tcgccaaggc tgccaagaag gcgggacgca tcgccgccga cggactgatc
     1681 ggcgttgcga tccaggcagg acagggcgcc atcgtcgagg tgaattccga aaccgatttt
     1741 gtcgcgcgcg gcgccgcgtt ccagaacctc gttcgtcagg cggcgcaaca ggcgttaaag
     1801 gcggaaggcg atctgcagcg ccttctggta tccccggtgg agggcggagc ctcgatggcc
     1861 gatgtgctct ctgcgctcgt cggtcaggtc ggcgagcata tgtcggtacg ccgcgcgagt
     1921 gcgctcgcgg tcgccccggg cgtggtggct tcctatgtgc ataatgccgc cgcgcccgat
     1981 ctcggcaaga tcggcgtgct ggtggcggtg gaatcctccg gcgatgcggg caagctgatg
     2041 gcattgggca agcagatcgc catgcatgtc gcggccgcca acccgcttgc gctcagcgtc
     2101 gatgcgcttg ctcccgacgt ggtgcaacgc gaacgcgcca tttatgccga ccaggcgcgg
     2161 caatcgggca aggccgagaa cgtcatcgaa aagatgatcg aaggacgctt gcgcaaattc
     2221 tatgaggaat cggttctgct gtcgcaggtg ttcgtcatcg atggcgagac accggtggca
     2281 aaagtggtgg agaatgtcgc gaaggacttg ggcgcgccgg taaagatcgc gggttttttg
     2341 cgttttgccg tgggcgaagg catcgaaaaa cccaccacgg attttgccgc cgaggttaaa
     2401 gcgactgcgg gtctctagcc tctcctgcag attctggaca cgatccgcac ggagcttcca
     2461 tgacggcctc cctcaaatat cgccgggttc tgctgaagct ctccggcgag gctttgatgg
     2521 gcgcgcaagc ctatggcatc gatgtctcga gcgtggagcg catagcggcc gacatcaaag
     2581 cggcggctgc tgcgggggca caactttgcc tggttatcgg cggcggtaac atctttcgcg
     2641 gcctctccgg agcggcggcg gggatcgacc gctcgacggc cgattatatg ggcatgctgg
     2701 ccacgatcat gaacgcgctt gcgatgcagg ccgcgctcga acgggtgggt cttccgacgc
     2761 gcgtgcaatc ggccatcccc atgatgtcga tctgcgagcc ctatatcagg cgccgcgcca
     2821 tccgccacat ggaaaagggc cgggtggtga tctttgccgc cggcaccggc aatccctttt
     2881 tcaccaccga tacggccgcg gccctcaggg cggccgaaat gggctgcgac gcgatgttca
     2941 aggcgacgca ggtggacggg gtctatagcg atgatcccaa acgcgtcaaa aatgccgaac
     3001 gctatgattt tctgacctat catgaggtat tatcgcgcga cttgaaggtg atggacgcct
     3061 ccgccatttc gctgtcgcgc gagaacgaaa tcccgatcgt ggtgttctcg atccacgaac
     3121 agggaacgct gtccgcgata ttgaaaggtc agggacgctg cacactgatc ggcgaacggc
     3181 gttaacgcgg gaatcgggag cggcgtctgc cgcttgcctt atgaagggag gtcaaaatgg
     3241 cgcagctgcc accattcgat aaggaagaca tgagacggcg catgaagggc gccgtggcta
     3301 cggccaagca cgaattcgcc ggcttgagga caggccgggc gaatccggcg atcctggacc
     3361 cggtgacggt cgaagcctat ggcagtgcga tgccgcttac ccaggttggt accgttaacg
     3421 tcccggagcc ccgcctcttg acggttcagg tctgggacaa gagcctagcg aaagcggtgg
     3481 acaaggcgat tcgcgatgcg ggtcttggac tcaatccgca actggatgga cagcttgtcc
     3541 ggattccgat cccggagttg aacgaggaac gccgccgtga attggccaaa ctcgcgcata
     3601 aatatgcaga agccgcgcgc gtcgcggtgc gcaacgtgcg gcgcgacggg atggatcatt
     3661 tgaagcggtt ggaaaaagag catgcgatag gagaagacca gcaccgccaa tcgtccgatg
     3721 agttgcaaaa gatgaccgat gagaacgtac atgaaatcga tgcggccttg accgcgaaag
     3781 aacaggaaat catgcacgtc tagagccggc ggggagtggg ggcggctcaa aggcgtgata
     3841 aggggaatcg acgtttgatc aaaacgatct tgaaatccgc cgtgccagaa tcgccctcac
     3901 ccagcaggtt gcccaagcac gtcgccatca ttatggacgg caatggacgt tgggcgaaaa
     3961 accggctgct cccacgcgaa gcgggccatg tcaaaggcat ttcctccgtc cgcaaggtcg
     4021 tgcgcgctgc acgcgagatg gggctgcaat atttgaccct ctacacgttc tcgaccgaga
     4081 attggcgccg tcccgagtcc gaggtccgtc atctgatggg actctttcgc cgctattgcc
     4141 gcgccgacat cgagaaactg cacaagcata atgtgtgcgt gcgtttcatc ggcgcgcgag
     4201 cgggactcga tcccgatatc gcggagctga tcgaggaggc cgagacgctg accgcgcgga
     4261 atacgacttt gaacctcgct tttgctttca actacggctc tcgcgaggag atggcggagt
     4321 ccgcgcgcgt cttggcgcgt gctgccgccg ccggaacgct cgaccccgaa cgtattacgc
     4381 ctgccatgtt cgggcagagc atgcagagcg cggggattcc cgatgcggat ttggtgattc
     4441 gaagcggcgg cgaaagaagg ctctccaatt tcctgctgtg gcaggccgcc tatgctgaat
     4501 tcgtgttcac cgataagctg tggccggatt tcggcgccgg cgatctggag gcggcccttg
     4561 ccgagttcgc tcggcgggaa cggcgtttcg gcggcgttgc ggcgatggaa cgcggacacc
     4621 gggaagcgtc cttggaggaa gccggcttgc gttcgctcgc tgccgaggtg ttcgatggcg
     4681 aatgagcttt aggaaacaac catcgtgacc gatacacagc cctccatgcc ggcccggttc
     4741 gagcttccgg cgcgcgcggc atcgggcgtt gtgatgggcg tggttgcaat cgccgcggtt
     4801 cttggcggtg gcattttctt cgcttgcttc gtggcactgg gcgccatcgc ggccttgcgc
     4861 gaatggcatc ggctggtgaa cggcggccat gtcgcgcgtg aaatgatacc cacgggcctt
     4921 gccgtcatct gcgcggtatg gctcgcgcgg gcacagaccg ggctgtcctg gccctttgcg
     4981 gcgattctgc tcggggcagc gggagcggcg attctgtcgg ggacccgtgg cttgcccccc
     5041 tattggccca ttccctggca cgcggtgggt gcattgtatg tgggcttgcc ggcgctcgct
     5101 ctcgtgctcc tgcgcgaggc gccgcgcggt gcggcgatcg taggggcgct cttcgtcgcg
     5161 gtctggatgg ccgacacggg cgcgcttttg tgcggccgcg tgatcggcgg tccgaaactc
     5221 gcgcctgttt tgtcgccgaa caagacatgg gcgggttttc tgggaggcac gatagccgca
     5281 gcgattggcg aggcgctgta tgtggcgatc ctgggcggca atgtaccaga cgcggtagca
     5341 ctggggattt ttctcgcgct cgccggccat tgcggcgatc ttttcgagtc ctgggtcaaa
     5401 cgtcaatttc gtgccaagaa cacaggccgg ttgatccctg gccatggcgg tatcctggat
     5461 cggatcgaca gcgtgttgtt tgcggctccg ctctgggctg gcctgatctt cctggccggc
     5521 tttaacccat ttaacggtgc tggcccatga agcctttggt caatcacgct ccgcctgatc
     5581 tcgatctgtc gcctgttctt ggccggcgta tcacggtgct gggaagcacc ggctcggtcg
     5641 gcacggcgac gctcgatgtg gtgagattcg tgcgcgcgcg ttatggcgcg gaagcgtttc
     5701 cgcttcaggc gctgacggca caacgcaatg tcgcgctcct cgccgcgcag gcgcgggaac
     5761 ttcgcccgcg cgtcgccgtg attggggatg tgtcgttgaa ggatgcgctt gccgatgcgc
     5821 ttgccggcac gggcatccgg gcgcttgcgg gacctgaggc gattctggat gcggcgcgcg
     5881 aaccttcgga tatggtaatg gccgccattg tcggggccgc cgggctcgcg cccacgctcg
     5941 aatgcgtgaa gcgcggtgcg attgtcgcac tggccaataa ggaatgcgtg gttgccgctg
     6001 gcgaagtctt ccgccgggcc gccgaggagt cgcacggcgt gctgatcccg gtggactccg
     6061 agcacaatgc cgcgtttcag cttttggact ttgcccaggc gcacgcaatc gagaaggtga
     6121 cgctgaccgc gtcgggcggt ccgtttcgta cctggtcgct ggatcgcatg gccggagcta
     6181 cgccggagca ggcggtctct cacccaaact ggtcgatggg cgcgaagatt tccgtcgaca
     6241 gcgcgactct gatgaacaag ggcctcgaac tgatcgaagc tcatattctg ttcggcctgc
     6301 cgcctgagac gctcgacgtc gtggtccatg cacaatcgat cattcactgc cttgtcgcct
     6361 attccgacgg ttcggtgttg gctcatcttt cggcgcccga tatgcggaca cccatcgcgc
     6421 acgccttggg atggccgcgg cgtattgcct ccccttcgcg ccgcctggat ttcatggaat
     6481 ttacccagct cacgtttgaa aagcctgatt tgtcacgctt tccgtctctt cagctcgcga
     6541 gagtttgctt gcagacgggc ggaccggccc ctaccatcct caatgccgcg aacgaggtgg
     6601 cagtcgagca gtttctggga cgccggatcg gttttctcgg gatcgcccgc accgtcgaac
     6661 aaacgctgag aacgtttgct cgcgaaatga acggccgggc gccggagacg ttggacgaag
     6721 tgctggcact cgatagcgcc gcacgggcaa gggctaggga ggcttctggg gtggccgcgg
     6781 cttgagaccg ctgcgctgat tgaggagtgt gagttcgtat gcctgctgtc gtcgccgatt
     6841 tcgtgtcctg gatgggcgtg gggcttcctg cctttctatt tgtcatcaca ctggtcgtgt
     6901 tcgtgcatga gcttggccat ttcttggtcg cgcgctggtg cggggtaacg gttgagacat
     6961 tttcaatcgg ttttggccgg gaaatcgtcg gcttcaacga ttccaagggc acacgctgga
     7021 aactctcctg gatcccgctc ggcggctatg tccgtttttt tggcgacgcg gatgcgtcga
     7081 gccgccccga ccgggaaaaa ttgaaatcca tcggagccct ggatgggatc ggcaagcggg
     7141 ttgaattcgt gaaggacccg cagagcgggg tgcttcattt caagccgctc tatcagcgcg
     7201 cgcttgtcgc tgccgcaggg ccgatcgcga atttcatcct ggccatcgtt atcttcgcgt
     7261 ctcttatcct gacggttggc aaacccgcgg agccgccgat tgtggaagcg gtgatggccg
     7321 gttcggccgc cgaaatggcg gggatgcaat cgggcgacac catacgctcc gtggacggac
     7381 aagcgattcg cgcattcatc gatctgcagc gcaccgtggg cgcgaatgcg ggaaagacat
     7441 tgcgtgtggt ggtgaaccgc ggtggacggg atgtcgcact cgatgtggtt ccgaccgcca
     7501 cggagatcac ggatgcttcg ggcgccaaac actcgcaggg ccggcttggc atcagggacg
     7561 ccaccgtgtc tgtgggcatt gtcgaggcta tcggaggcgg actcgaccag acctggttca
     7621 tcgtttcgca gaccggttca tatctcggaa agattgttac cgggcgggag agccccgatc
     7681 agttgcacgg catcctgggc atcggcgcgg tatcggatca ggccgcgcgt attggatttg
     7741 cagcgttgat cggtcttgcg ggtctgatgt ccgtgagtat tgggttggtg aatcttttgc
     7801 ctattcccgt gctcgacggc gggcaccttc tgtactatgc attcgaagcg gttctcggta
     7861 ggccgctggg ggaacgcgca caggaagttg gtttcaggct gggcttggcg tttgtgctat
     7921 gcctcatgtt gcttgcgaca ttcaacgacc tggtccgctt aaacctcttt tgatcgaatt
     7981 tgcgctgcct ccgctaaaag ccgaggcatg atactcaaag gcgagtcgat gaccttagac
     8041 ggccacccta tcgcccggag cgtccggttt gttgtctggg cgttggtggc gtcgtgttcg
     8101 gtcccggcgt tcgtaccggg gcccgcgttt gcacagacag acgcgccagc ttccgtgccc
     8161 gttcctcccg ggccgcagat gaccctcgat caggcagccc cggtcatgat gccgatgccc
     8221 gcgcctccag ggcaagggat ggtccagcgc atcgttgtcg aaggcaccca gcgcatcgaa
     8281 ccggacagcg ttctttccta tctcctgctg cgccagggac aggcctatga tcccgccgtc
     8341 gcggaccgtt cgctgaaggt gcttttcgac accggactgt tttccgacgt gcggatggac
     8401 tttgacggta cgacgctcac ggtccgtgtg gttgagaatc cgattctcaa ccaagtggtc
     8461 tatgaaggga attcgaacac ttcggacgcg gatcttaaca aggaggttca ggttcgtccg
     8521 cgcatggttt tcacccgtgc gcgtgtccag gccgacgtgc agcgcatcat cgaactgtat
     8581 cgccgggccg ggcgttttgc cgcgaccgtc gaacccaaga tcatccagcg tccgcagaat
     8641 cgcgtcgatc tggtgttcga gattaacgaa gggccgacca ccggcgttgc cgccatcaac
     8701 ttcatcggca accgtgtgtt ccccgatacg gatttgcgtg aacagatcgc cacgacgacg
     8761 agcgcgtggt ggaaatttct caccaccaac gacaattacg atccggaccg gttgaccttc
     8821 gaccgcgaac agcttcgccg cttttatctg ggacggggat atgccgattt ccgcgtggtg
     8881 tctgcaatcg ccgagcttgc cccagaccgg aagaatttct acgtcacatt cacggttgac
     8941 gaaggtgagc tttatcattt cggtaagatc cagatcaatt cgcgcatccg cgacctcaat
     9001 cccgccgatc tgcagccttt gctgctgacc aatgaaggcg atgtctatga tgcgggcttg
     9061 gtggacaaat cgatcgaggc tctgacattc gcggccggaa cacgcgggtt tgtcttcatc
     9121 gacattcgtc ctcgggtgca gcgcaatccc atgaccaaga ccgtcgatct tttctatgag
     9181 atcgacgagg caccccgggt ctacatcgaa cgcatcaata tcgtcggcaa tacacgcacg
     9241 cgcgatgagg tgatccggcg cgaattccgg cttaccgaag gcgatgcctt caaccgcgtg
     9301 ctcgcggacc gctcgcggac ccgcattcgg gcgcttggat tcttccgcga tgtgaccata
     9361 acggaggatc cgggttctgc gcccgaccgg accgtgctga cggtcaacgt gaccgagcag
     9421 cccacgggtg aactttcgct tggcgcgggt tattcctccc agcagggatt gctactggat
     9481 ttcagctaca ccgagcgcaa tctcttcggg cgcggacaat tcctgcgcgc aagcgtttcc
     9541 atcggcacgt tccagaagga ctatgatttc cgcttcaccg agccgtattt tctcggccgg
     9601 ccgctggccg cgggcttcct ggtctacaag gtgatcaccg atttcaccga tcagatcggg
     9661 tatatttccg atgtcaccgc cgcgggaatt cagttcggtt ttccggtttc ggaatttggc
     9721 cgcatttcac cgcattataa tttctcgtac acgcgtatcg attcgacagt tacggcttcg
     9781 gaggcgatcc agctcttccc cgcttactcc gcttcgtccg tcggttacac ctacacttac
     9841 gacacgctcg atgatgcgat taagccaacc aaaggctgga attttgccct cagccaggat
     9901 gtttcgggcc tgggtggcga gctcaaatat ctgcgttcgg tggcggccgt ggaatattat
     9961 catcccttct ttttcgatct cgtcggcgag tttgcgttga atgcgggata tatcagcggt
    10021 tatggcgggc tggccgtgcc gatcaacgag cgcttcttca agggcggacc gtcttttcgt
    10081 ggctttgaga tcgccggcat tgggccgcgg gatctggtga gcgattccgc cctgggcggc
    10141 cagttctact tgatcggcac ctatcaggtg agattgcctg acatattgcc gccggattat
    10201 ggcatcaatc tttcggcgtt ttccgatttt ggaactctgg gactggatcg gagcagggca
    10261 ctcgaatgcg cctcggcggc cagttgcaag gacgatctgg cccctcgcgt ttcgctcggg
    10321 atcgccatca actggaaatc cccgttcggc cctgtagaag tcgatgtggg ctatcctata
    10381 atcaagcagt cttacgacaa gccgcaggcg atccgcctga gtgcaggcac ccaattctga
    10441 tgtgagaagg acgcgtagaa tcatgagcaa gacacatcat tgccgcacag cggccgctat
    10501 tgcggcgctc acgctcgggt ttttgatctc ggggtcgcag gagggcccat ccggcgcaca
    10561 agccgcgccc gcgcctgcgg caaccgctcc tggggtcgtg gtggcgaggc ctgccacagc
    10621 gcccgtatcg accgcgcctg cccccgccgc gcctagcgtt gcggccgtgg ccggcgccgg
    10681 aaccgtgccc cgcatcgtgg tcatcgatcg caattttatc ttgcagcggt cttcggcggg
    10741 gcaggacatg atcagccaat cccagaacct gtcgaaacag gcggaaacgc aattcaaagc
    10801 ggaggaagcc gcactccaga ccgaaatggg acagttgcag cagcaactcg caatcctggc
    10861 gcccgatgtc cgcgatcaga aggaaaagga tttcaccgcc aagcagcaag cattccaggc
    10921 aaaagtgcaa cagcgccagg cggaaattca ggccggcttc aacaaagccg ctcgctcgct
    10981 cgaggtcgcc cttgaaccga tcctgaagac tatcatgatg gagcgcggcg ccaacatggt
    11041 gctggaccgg acgtcggtca tcctttcgtc cgtcgatgtc gatgtgacgc ccgtcgcggt
    11101 gcagcggctc gacaaggcat tgccccatgt gaaggtggaa ttgacagcgg ttcctgcgac
    11161 ggccgcagca cgccctgcgg gggtaccggc cttaaccccg gcggcggccg cggtgaaaaa
    11221 gtagcggcaa cacgcagtct caacgaaggg agtgccggat ggcggatccg cggttctatg
    11281 acaatcgcgg cccgttccat ctggccgaac tgtgcgtcag cgcccgcctt gccccccctg
    11341 cggactctaa cgcggacgcc ccgatctacg acgttgcggg gctcgaagag gcgggaccgc
    11401 cgcatctcac cttctatgac ggccggcgca acaaggcggg atttcttgcg accaaagccg
    11461 gttggtgcct tgccgacaac cgcttgatcg aaaccgcgcc tgcatcgacg gttgtcatcc
    11521 cggtgccctc cgtcgcccga gcgtttgcag ccatggcggc gcgcttttat cccgagcacg
    11581 aattggatat tcgccggctg gaaagcgcgg tgcatccatc ggcgaagctc ggtcaggacg
    11641 ttgtgcttgc tcccggcgtg ctgatcggcc ctgacgccga gataggcgat ggcgcgcgca
    11701 tcggtgccta tgccgttatt ggccggggcg tgactatcgg acgctccgcg gagatcggtc
    11761 cgcacgcatc catcatgttc gcgcatctgg gcgatgatgt actcatccag gctggcgcca
    11821 aaatcggtgg ctccgggttt ggctattcct cagggcccga tggccacatc aagatgccgc
    11881 atctggggcg cgtgatcgtg caggacggcg tggagatcgg ggccaattcg acggtcgaca
    11941 gggggatgct cagcgatacg gtcatcggcg aagggaccaa aattgataat ctggtacaga
    12001 tcgggcacaa caccgtgacg gggcgtcatt gcgtgctcgc cggtcaggca gggacctccg
    12061 gcagtgtggt tttgggtgat ttcgtgattg ttggcggcgg agtaggaatc tccgatcatt
    12121 tgacgatcgg agatcgggca cggcttgcgg gccactccgg agtcgcgcac gatctcgaag
    12181 ggggccgcga ctatggcgga atccccgcgc gtccgatccg tcaatggcac aaagagggtc
    12241 tcctcatcgc gaagctcgcg cgcaagggtg tacggtttac ggatggataa gaaaacggaa
    12301 agcccctcga tacagatgga aattggcgtg gagcagctga aaagactgct gccccaccgg
    12361 ccgcctttcc tgatgattga cagattgagc gacatcaaag cgggcgcatc cgctgtcggc
    12421 tggaaagcgg tatcgatcaa tgagccgcat tttgtgggcc attttcccga atatgcggtg
    12481 atgcccggag tgcttatcgt tgaagccatg gcacaggccg cggccgcctt cgtggtgcac
    12541 acactggggc tgcaggatca gcggcgcatg gtctatttca tgaccatcga caaagcgcgc
    12601 ttccgccgtc cggtgcatcc gggcgtcgtt ctgcgaatgc cggtccaggt gcagcggcgc
    12661 cgggggccgg tgtggcgctt cgaaggcaag gccttcgtcg gcgaggagct ttgcgcggaa
    12721 gccgaatata gcgccatgct gacggaacat agtttgggga tggtgggctc gccggtcctg
    12781 tccggcggcg cggagtgatg tcgaagatcc acccgaccgc cattgtcgag gacggtgcgg
    12841 ctctcgggtc cgatgtcgag atcggtccgt tttcgctgat cggcgggcac acgcgtttgg
    12901 cggacggcgt acgtattctc tcccatgtcg taatcgcagg ccatacggag atcgggcgcg
    12961 cgagcgtcgt gcacccccat gcggtgttgg gcggcggccc gcaatttcgc ggcgatacgg
    13021 gcgcggaggc gcgtcttcgg attggcgctg aaaacattat ccgcgaacat gtcacgatga
    13081 atggcggctc cgtcaaaggc ggcgggctga cgcaggtggg agatcgcggc tatttcatgg
    13141 cctatagcca cgtcgcccat gattgccaca tcggcaatgg cgtcactttt gcgaacagcg
    13201 tggcactcgg cggtcacgtt acgatcgagg acggcgtcaa tattggcggg ctctccgcaa
    13261 tccagcaatt cagccgcatc ggccgttacg cctttgtcgg tggggtgacg ggcgtcccgg
    13321 acgacgtcat tccttacggg atggcgtggg gcgatcatgc gcggcttgag gggctgaacc
    13381 tgattgggct caagcgcagg ggcatcccac gcgagcgcat ccatgcgctg cgcgccgcgt
    13441 tccgcgcgat cttcttcggt tccggccgtc tgttcgagag agcgcgcgac gccggccagc
    13501 gctggaagga tttccccgaa gtgagtgagg tcgtcgcgtt cattctcgcc gacagcaaac
    13561 ggcccatctg catgccgtcg gctacgcacg ccggcgccga gacggggtga gaggcatgga
    13621 tgcgccgggt ggaacgcttg gaatcatcgc cggcggcggc gatcttccca ttgtgatcgc
    13681 ccgcgccgcc caggccgatg gccgcgcggt cttcgtgctg gcgctcgcag gcatggcgaa
    13741 cccgtcggat gtcgccgcct ttccgcacgg ctttgcatcg ctcggtgagc tggggaaggc
    13801 cattcgcctg ctcaaggatt cgggctgctc cgaggtgacg tttgcgggca aggttgcgcg
    13861 gcccgaattt tcaaagctca agctcgatgc ccgcggcgct ctcgcattgc cgcgggtgat
    13921 ggcggcggcg ctgaagggcg acgatgcgct tctacgctcc cttgtcgcga tcttcgagaa
    13981 agaaggtttt cgcgtcatcg gttcgaccga tgctgcgtgc ggactcgtgg cgcctttggg
    14041 gacgctcgga aaattcgcac cggctcgcga ttctgatggc gacatcctgc acgccatccg
    14101 ggttgtcgcc gcgcttggcg cgttcgatat cggccaggcc gccgttgtct gcgagggcct
    14161 tgtgcttgcc gtcgaagccg ccgagggaac cgacgcgatg ctggcgcgcg tggcggccct
    14221 tcccgaagcg ttgcgcggca cgcccgaaca tcgcaaaggc gtgctggtca aggccgtcaa
    14281 acccaatcag gaacggcgcg tagacctgcc ggttatcggc gttcgcacgg tggaattggc
    14341 ggcagccgcc gggctttcgg gaattgcgat cgagggtgct gccgcgttga tcgtggatcg
    14401 cgcgcgcgtg acacaaacgg ccgatcgttt tggaattttc atctacggtt ttgcgccgga
    14461 ggattatccg cggtgagcgc ggatgcggaa tccagaaccc tcaccatcat gctcgtagtg
    14521 ggcgaaccct ccggcgacaa attgggcgcg cagctcatgg ccgcgctcaa acaggtcgcg
    14581 ggaaaacacg tgcgcatcgt gggcgtgggc ggtgctgcca tggccgccga aggactcgag
    14641 agtctttacc cgctcgacgc cacgtccgtc atggggcttc gcgaagtcgt gccgcgcatt
    14701 cccgcaattc tcagacgtgt gcgggaggcg tccgattttg cgattctgac gcgtcccgat
    14761 ctggttgtgc tgatcgacag tccggacttc acccaccgca tcgcccaacg cctgaagcgc
    14821 atcgatccct ccatccgcac cgccaattat ccgccgccgc aggtctgggc ctcgcgcgcc
    14881 tatcgggcgc ggaaaatggc gcgctatatg gatgcggtgc tgacgatgtt cgcgttcgaa
    14941 gcggccttct tcgagcgtca cggcatccgg gcctacaatg tcggctatcc cgtgatcgag
    15001 cgcgcggaca agattgccgg cggcgcggaa tttcgaagac gccacgggat ttccgccgat
    15061 gcgccattgc tggtggttct gccgggaagc cgccgcaacg aaattcgctt catcctgccg
    15121 ccgttccgag ctgcggtcga aatgctggcg cgcgatattc ccggtctggt cacggttctg
    15181 ccgaccgtca gccatgttgc ggctcttgtg aaagccgcag tcgcgaagtg gccgacgccg
    15241 ctgcacatcg tggatacgga gccggaaaaa tacgccgcct tcgatgccgc ggacgcagcg
    15301 cttgcggcct cgggaacggt gaccagcgag cttgcacttg ccaaggtacc gatggtggtg
    15361 gcctacaagg tcggctggct gacctatgcg ctcgccaagc ctttcatgca tgtgcggtat
    15421 atggtgctgg tgaatctggt gctggggcgc aaggccatcc cggaattcga gcaatatgcc
    15481 gccacgccgc aagccttggt gcaggcgtta aaaccgttgc tgaccgacga atccgcgcgc
    15541 gccaatcaga tgcgcgatct ggaagaggga gtacgcgctt tcggcatggg gcaggagacg
    15601 ccgtccttgc gcgccgcccg cgtggtgctc gaactggcgg gagcgaaatc ttcctaatcg
    15661 cgtctacttc acttgcaatt cccgtttcaa aaacacaacg gcgcgattga gcgcatcggt
    15721 cgcgttcttg tcgtcccagc gtgcaccgaa gccatgcgac tcgccgggat agatcacgag
    15781 atcgaccttc gcgcccagct tcttggcttc atccgcaagt tgcttgccgt tttcaaccgg
    15841 gatgatctga tcggcatcgc catgcagcac cagaagcggt ggaaagcgct tggccggctg
    15901 acccagtggc caggggtcgg tgccgtaata aatcaccgcg gcattgatct tgggatccag
    15961 cgtggaggcc ccggtcgcga gaattccgcc attggaaaac ccgacaagtc cgaccttgcc
    16021 gttggagtct ttctgcgcga tcaaataatc ggcgaggtcg tcgaccagct tcgcccattg
    16081 cgggtagcgg tcttcgaaga cattgacgcc attagacatc atcttgtcgt ccatgtcgct
    16141 gtaataatag acaagatagg catcgatgcc ttgggcggcg agcgcggcgg catagcggtt
    16201 gttgttcgcg atttgccggt cgaagccgcc cgcgccatgc agcaggagcg ccgagggacg
    16261 cgggccgttg ccgggtgcgc ggaaataggt gacatgaacc tgtcgctcct tcgcgggcag
    16321 catgatcttc tgcccctgga tttcggcggc ggacgcggct gatcccatca gcgtcaaaat
    16381 cgtcagaggc gccaaaaagc ttcgcgcgat cgagttaatg gggttcactc gcctttgagc
    16441 tgtttgtcga ggaatgcaat cgtatgcttg aacgcttcgg cggccgcggg atggttcaaa
    16501 tccgcggcga agccatggtt cttgcccggg aacgtcaccc actccaccga ggccccgacc
    16561 gctttggcct gatcgaccag ccgctgtccg agcgccatcg gttgccgcgt gtcggcctcg
    16621 ccatgcatca cgaggagcgg cgggaagcgc gccggcgcat agcctggcac acgtgagacg
    16681 ccgccgtaat gaatcacggc cgcgtcgata ttcgtgtcga gcggggttgc atgcgccgac
    16741 aggttaccgc catttgagaa gccgacaaga ccgaccttgc cgttcgagcc cggcatcttc
    16801 ttcacatcgg cagtcagatc gctgacgagt ttcgcccagg ccgggaagcg cttttgcgtg
    16861 tagccgccgc gcgtcttgtt gtcggccatg atcttggcgt cttcatcgct gtagtaatag
    16921 accatgtagg cgtcgaagcc gtgattggcg agttcgctcc catagtgacg gaaattctcg
    16981 atgcgcccat ccggcccgcc ccagcccgcg ccgccatgca gcataagcgc ggttggacgc
    17041 ttgcccgtac cgggcgcctt gaagaactcc accttcacgg cgcgttcggt ggcgggcaag
    17101 gtcatttcct tgccctggat ttcccccgcg ctaacgcttt gcgcaaagcc tgaagacacc
    17161 agcagcgcaa gtgctaccgc gaaatgtctg atcgggttca tggcgtcatt tccctttcaa
    17221 agtcttgtcg aggaaagcaa gcgtgcgctg aaacgcatcg tcggcggcag gcgtgccgag
    17281 attctgcccg aagccgtgat tgattccggg ataggaatga aattccacgg gcccgccgag
    17341 cgctttcacg gtttcataaa gtttgcgccc gcgttcgatc ggctcacgcg tatcggcctc
    17401 gccgtggaag atcatcatcg gcgggaaacg cttggcctca tagccggaca cgcgcgacac
    17461 gccgccataa tagacgacgg ccgcgtcgat attcttgtcg agcggcgttg catgggccga
    17521 aagattgccg ccattggaaa caccgaccaa gccaaccttg ccgttggaat cggtcatctt
    17581 cttcacatcg gcggtcagat cgctcacctc ctgcgcccag gcgggaaatc gcttgacgct
    17641 gatgcccggc gcattcgcct tgcgatcgat gacatcctga tcgctgtaat aggagaccat
    17701 gtaagcgtcg aagccatgat tggcgagctc gctcgcataa tgttgaaaat tctccgtgcg
    17761 tccctcggca ccaccccaac cctggccgcc atgcagcatc agcacggtgg gacgtttggc
    17821 attgcccggc gcgcggtaga attccacctt caccggatgg tcgcttgcgg gaaggaccat
    17881 ctccttgccc tggatgtcgg ccgcattcgc gttacccttg atgtcggccg cattcgcggc
    17941 aggcgcgagg acagaaacag caagcagcgc caggggcgcc acaagattcc gtgccaaatg
    18001 gttcatggtc gagcccgctt tcagtttgag ttgcccaagg gcgtggaagg accggtgcca
    18061 tacgcggggt gatgcgcgta acggttaaat cccaaggtga ccagcatcag cgtaaaggcg
    18121 cccatgacgg cagcggcaac cagccgccgc catttcgggc ggcggcgcgg gttgtcgttc
    18181 gcaggccggg tcgaggatgg agaataatga tctgcctcgc ccggatgcat cctgtgcctc
    18241 ggctagccgc gtttctccgg cggaatataa tcccgccgat gggggcccgt atagatttga
    18301 cgtggccggc cgatcttctg ctccgggtcc tcgatcatct cctgccattg cgccacccag
    18361 ccgacagtac gggcaagcgc aaacaaggcc gtgaacatgt cgttggggaa acccaaggcc
    18421 ttcagggtga tgccggaata gaaatcgata ttgggataga gcttcttctc gatgaaataa
    18481 tcgtcctgca gcgcaacgcg ctccagttcg agggcggtct tcagaagcgg atcgtcatgc
    18541 ttgccgagct cgtccagcac cgcatagcac tggtcgcgca ttactttcgc ccggggatcg
    18601 taattcttat agacccggtg cccgaagccc gacaggcgga acggatcgct cttgtccttc
    18661 gcgcgcttca cgtattccgg cacccggtcg atggtgccga tctcctgtag cattttcagg
    18721 gctgcttcgt tggcgccgcc atgagcgggg ccccagaggc acgcgatgcc tgccgcgata
    18781 caggcaaacg ggttggcgcc cgacgaaccg gcgagccgga cggtggaggt ggacgcgttc
    18841 tgctcatgat cggcgtgcag cacgaaaatc gtatccagtg ctttggtcag aaccggattg
    18901 actttgtatt cctcgcacgg caccgcaaag cacatccgca tgaaattttc cgtgtagccg
    18961 agatgattga gcgggtacac gaagggctgg ccgatggaat atttgaaggc catggcggca
    19021 atcgtcggca ttttggcgat caaccggtgg ctggcgatct cacgctggcg cttgtccgta
    19081 atgtccgtgg aatcatggta gaaggccgag agtgcgccga cgacccctac catcaccgcc
    19141 atgggatgcg catcgcggcg gaatccccgg aacaggaagc tgatctgctc gtgcaccatc
    19201 gtgtggtaag tgatggtgtt cttgaaatcc gaagattgtt tgggtgtagg aagttcccca
    19261 ttcagcagca gataacagac ctccaggaag tttgagttct tcgccagctg atcgatggga
    19321 tacccgcgat agagcaggat gcctctgtcg ccatcgataa atgtgatgtc gctctcgcac
    19381 gacgccgtcg acgtgaagcc cggatcgtag gtgaaaaggt ccgcttcgcc gtagagcttg
    19441 cggatatcca cgacatcggg accctcggtc cctttgtaga ccggaaggtc gactttcttg
    19501 ttcccgtaat tgagcgtcgc cgtcccgttt ggcttggcgc cgctggcttt tatgttcatt
    19561 tcaggactcc gtaggttatg gagcggccct cccgaaaggt ccgtgtcgtc tgtttaaacc
    19621 cagcgcctta gttaatttcg ctgtgaagcg tattcgcctc acatgcaaca cagtttaatc
    19681 gccttgggcg cgaatgcgag tcaaggcttc ttctcgtccc aataccgcca gcacgtcgaa
    19741 gaccgggggg gaggccgtcc ggccggtcag tgcggctctc aagggctggg caatatcgcc
    19801 cagtttgatc ccttcgcgtt ccgcaaatcc gcgcgtgcat gcttcaatgg cgcttgtgtt
    19861 ccaatcggcg ccctccagct ccagggcgag cctgcggagc cagccccgtg tttcaggtgt
    19921 cagcgccgcg gcggccgcct tatcaattgc gggcgtgccg ctgatgaaga ggaaatcgcc
    19981 cttctccagc agttcgacca gtgttttggc ccgcggtttc agaaacggca tggccgcggc
    20041 gaggcgcgcc cgcaggatag cgtcgaagct gcggccggga ccgatccgtt gggcgaaggc
    20101 cagcgtctcg ccggcgagcg ccccatcgga catttcccgc atgtaatgtg cgtttatgct
    20161 ttcgagtttc ttcaggtcga agcgagccgc cgaccggccg atgccggcga gatcgaacag
    20221 ctcgatcgcc ttttctgtcg gaatgatctc gtcgtcgcca tggctccatc ccagacggag
    20281 aaggtaattg cgcatcgcct ccggcaggta gcccatatcg cgataggcct ccacggcgag
    20341 cgctccgtgc cgcttagaga gcttcgcgcc atccgacccg tgaatcagcg gcacgtgacc
    20401 gtagaccggc accggccagc ccatggcttg gatgatctgc aattggcgcg cggcattgtt
    20461 gagatggtcg acgccgcgga tcacgtgggt cacgcccatg tcgaaatcgt cgaccacaac
    20521 ggccagcata taggtgggcg tgccgtcgct gcgcaaaagc accatgtcgt cgagctgatc
    20581 gttggcccag cgcaccgagc cctgcaccac atcatgaacg atcgtctcgc cggtttgcgg
    20641 ggccttcagc cgcaccacat acggcatatt gtgctgttct ggccctggcg tgcggtcgcg
    20701 ccagcgtccg tcatagcgga tggatagacc cttggccttc tgctcggccc gcatctgttc
    20761 gagttcctgc ggcgtcgcat agcagcgata cgcctggcct ttggcgagca tctgttcggc
    20821 aacttcccga tgacggctta cgcgggcgaa ttggaaagtc ggttctccat cccaatccag
    20881 ccccatccag ctgagcccat tgaagatagc cgccaccgct tcgggcgtgg aacgctcgcg
    20941 gtcggtatcc tcgatgcgca gcaggaacgt gcccccgcta tgacgggcat agagccaatt
    21001 gaacaaagcc gtgcgcgctc ccccgatatg cagatagccg gtgggcgaag gtgcgaaacg
    21061 caggacgggt acggaatttt cagccattat gatacgtctt gggggttgct cgcttctagc
    21121 aaaggggggc cattgcggat aggggcctct ggaatcgggc tgacgcgccg ttggcgcgca
    21181 tttgcagcac tcacgtggcc gcaacaggcg tccgcgaatg ctgtttcgtt tcctggcatc
    21241 gcgggcgctg ccgagccgcg ttttcatgcg cgcgcgcttc aggtgctggc gaatgccgcc
    21301 gaacgcaatc tcgccgccga acaggagcgc tggcttgcct ggtgggtcgc cggcttcgcc
    21361 gccgggatat tgatctattt cgctctgcag accgagccct cgcttcccat gacggcgctg
    21421 gtggttgtga ccgcatcgct ctgcgcttgg agcaggccca gaaacgcggg cacggcgatg
    21481 cggttcttct gcgccatgat cgccgcgggc ggtctcgggt tctccgccgc caagctccgg
    21541 acccttcgta tcgacgcgcc catcattgcc cacgatatgg gcccggtccg gctgaccggg
    21601 cgcatcgaaa gcgtggacat ccgctcggac aaacgcgcgc gcatcgtgct tgctcctgcg
    21661 acgctcggag atgggaaaac ggcgaggccg gaacgtgtgc ggctgacatt gatgggagcc
    21721 aaggccgtgg ccgcggtgca gccgggtgct gtggtatcgg cgctcgccgt gctgcggccg
    21781 ccgcctgaac ctgccatgcc gcatggctat gattttgccc gctgggccta cttcaacgca
    21841 atcggcggcg tcggatttac ctaccgcgcg cccaagctgc tcgacgcgcc gccgccgggc
    21901 cttctcgatc tggcgcgcgc gcgggtggag aatttgcgcc ttgccatgac gcgccgtatc
    21961 acggccgcaa tccccggtgc cgatggaaac atcgccgcag ccctcatcac gggcgagcgt
    22021 ggtgagatcg acgaagacga caaccaagcc tatcgcgatt ccggattggc gcatgtgctg
    22081 tcgatttcgg gcgtgcatct ggcactcgcc ggtctcggca ttttctgggc gctcagagcg
    22141 ctcctcgcgc tgtggccctg gctggcgttg acgcagccca tcaagaaatg ggccgccatc
    22201 gccgcattcg caagctcgag cttctatctc gccatcagcg gagggggatc gcccgcggtg
    22261 cgctcctatt tgatgctgtc gatgatgctt ttgggcgtgc tggccgaccg tcctgcgctt
    22321 tcgatgcggg cggtagcgct cgcggcattg gcgctgctcg cctatgagcc ggaggacatc
    22381 gtggacccga gtttccagat gtcgttcgcc gccgtgatcg gcctgatcgc gctggccgaa
    22441 tggggcgcgt cggttgtgcg caacgacacg ccggctcctg ggcgcggcgc ccatttctgg
    22501 cgcaaggggc gccgctatgt ctttgggatg ctgctggcga gcgcggtggc gacgctcgcc
    22561 agtacgccgt tcgccatcta tcatttcgat cgtgccgcgg cctattcgct gttggcgacg
    22621 attatagact tcccggaagt tcccggaaag tcccggaaag tcccgtaaat cctgggtttt
    22681 tctgttccaa attcacaacg caatggtact ctccaggatc gatttgctgg cggtaacgag
    22741 acacataccg ccagcaaaac cgccaccacc gggggacacc attgattacc gaatctcaga
    22801 ttaaatccgc gctgcgcgcc gcgccgactt gcggcaaaaa atcgatcgaa ttgaaggatg
    22861 gcggctcgcg cggggagggc aggttggcct tgatcgtgcg ggctggggag agccggataa
    22921 ccacagaatg gtacgcagtc tattaccggg gcggtaaacg cacgacgacg aagatgggat
    22981 cctatccgac gtacggcatc gccgacgcgc gcaagctgtt ccgtgaagaa tatgccccga
    23041 aaatctctag cggtgagaat ccgactggtc ctcggtcgcg cgcgaacaaa attggcgtca
    23101 cggtactcga tctgtttcag gcctatgtcg cccatcttaa ggcgaacgca agtccacggt
    23161 cggccgtaac ggcgcgcttc attcttctcg gccccccgag tcgccctggc ggcgccgcgc
    23221 gtgccatcgg agaagacatg cgcgcagccg acgtacggcc ggctgacata acgcctcacc
    23281 tttccgaaat tcatgctcgc ggctgcgtgg ctctggcggc cgatacgcgc acctatataa
    23341 gcgcggcgtt cgcgttcgtg atcaagtcgg ccaattccta tacgcagccg gccggggcga
    23401 caaagtgggg gatcgagacc aaccccgtcg cggcgatccc ggctgatcct gcgggtgccc
    23461 gcatcggcga tcgtcatttg tcgccctctg agtttcgcgc gttctggagg tggctggagc
    23521 gcgaggatag caacagcgct gtcgcgccag ccttgcgcct gatcatggcc acgggtcagc
    23581 gcgtgacaga aattctggaa ctcacgaata ccagctatga tcgcggggag cggatgctcg
    23641 actggcggaa gacaaagaat ggaaggcagc attcgattcc actgcccacg caggccctat
    23701 caattctcga tgggctagtc gtcaatcgcc atgggctgtt tttcccgcac ggacggcggc
    23761 ctgacgagcc ggcaacaatc tactctccac cgaaggtgat ctatcactat gcggaggata
    23821 ctggcgccgc gccattcacc gcgcgtgatt tccgccgtac gtggaagacc ctcactggcg
    23881 cggccggtct ctcgaaggac atccgtgatc ggctgcaaaa ccacgcccgc caggacgtat
    23941 cgagccggca ttatgaccgg tatgaatacc tgcaggagaa gcgcgcggcc atggcgacgt
    24001 ggtcggcgta cctcgacagg atcctcgccg gcgagttcga caaaaatgcc atgaaagagg
    24061 cggcgtgatg aagcgtgctg caatgtacgg ggagccagac cacggaaaat caagagcgcg
    24121 agttgcgcgc ggtcgctatc ggccgaacga gagatcgagg ccgcgctgaa ccgtggcgat
    24181 cgcgggatca ggaagatcgc gcgtgacgtt ggagttgggg tcggcacggt ccaacgtatc
    24241 aaggtggcga gtccccatgg gtagcgaagc ggtctcgcgg ctgacgatgt gtccggccag
    24301 cgaataaagc ctgcggcgac gattcatttc gccgcgggcg cgcctcgcac gcgacgcaaa
    24361 acgatattcg cagagatttc ggaaatttaa tctaagcctc ccgacacttc ccggaacttc
    24421 ccggaagttc ccgtgacttc ccgctgtgac gggatgtgaa cgtgttttcg agcgcgtcaa
    24481 cgacgtttcg aatctttccg tgcgcagaaa tctcgccgtc gtgactccat gctcgggcat
    24541 cgatgatccg ggaacatgac tatgaaagat tttgcagaga cattccgcca aacgattgat
    24601 gaaatccacc gcgatgcggc ggacctgggc gtttcaatgt cgagggtgtg cgggagtgca
    24661 ggcgtagctg aacccacccc gcggacgtac cgccgacact tgcctacgac cgtacggacc
    24721 gtggtgaaat tgcaaaacga aatcgaacgc cgccgtgcgt tgagggcggt gagcgatgcg
    24781 gcataaaaaa acgccgcctc ctgccaggga gacggcgctt tcgatctgct tgccacggca
    24841 cgaatcgata atcaaaggat attgtcacac cgcggcggcg caagtgcgcg tgcctgacgt
    24901 cgctcgcgca gttcgcgtcg gtggtggtgc catgagtggg gcgaacaccg aaattgcggc
    24961 cgttcgcgac cgcgtcgacc ttgctgcgct gatcgggcgc cgcgtgaagc ttgcgcgcaa
    25021 gggtcgtgcg tccgttgggc tatgcccgtt ccacagcgag aaatcagcgt ctttcaaggt
    25081 tgaaaatgga cggtttattt gctttggctg cggcgcacgg ggcgattgca ttgatttttt
    25141 aatgcaggcc gacggtctat cgtttcgtga agcgctgcgt gcgctttcgg atcaggtggt
    25201 ggcaattcca ccacgggccg cggcgcgttc ttcgccggaa gcttggccga aggacgatgc
    25261 gaagcggatc gccggtgcga ttcggatttg gaatgagtct ggcgctctcc gcgggacccc
    25321 gggttggcgc tacctccaaa gtcgcggcat tgacaccgat gcattgtccg atcgcctcaa
    25381 tgaagtcctt cgctggcacc cgcgttgccc gtggaaggat ggcacgcacg gttgtgtgat
    25441 cgcgctgtgg accgacgcgc tgatgggcga accgcgggcg attcatagga ctgggattac
    25501 agcgggcggc gagaaaatcg gccggctcgc gcttggtccc aagaagaact gcgtcatccg
    25561 gctctggccc gaagtcaccc aatcgctggt cgcgggggag gggatcgaaa ctgtcctcgc
    25621 ggccgcaaca cggctgacgc atcgcggctg cccgctgcgg ccagcttggg ctgttggtga
    25681 cgcaggaggg ctcgcggcgc taccagtgct tcccggtatc gagtctctga cgctgctcgt
    25741 ggatcacgat atcaacgagg tcggacagcg cgctgcagca acttgctcaa gtcgctggac
    25801 tgccgctggg atcgaggtgc gtcggttgct tccaaaggcg cccgactcgg atttcaacga
    25861 cgtcgtcgta cttaacggtg cctggcatga gggataatcg ggacgcgctc gccgaagcgc
    25921 tgggcatgcc tccgggaccc gaagacaagc ttcgtctccc cgcgtttgcc gttccaaaga
    25981 tcggcggccc acaggcacgc gaatacctgc agcgcctcgt gcacaagtcc cttgatctga
    26041 ttgtagaaca tggtgaagcg aaggcctgga ccgagggtcg cactgaagaa ttattatccg
    26101 cctcagccga tcccgacgga gagcgtgcga agattctcag tcgggcgaaa cgggaggcga
    26161 agcgccgttt cacggcggaa gtcttgaagc cgcttcgtct tcagatcgcc gcgtccgcgg
    26221 gggtagggaa aactaccgcg gtggcgaacg gttacaggaa caaaccggcg ctttggcgaa
    26281 agtcgatctg gtacttcgcg cccacagtcg acctctgcga acaggttgtt cgagacatta
    26341 atgaaaatgc tccccgcggc atgccgctcg ctcgcgtcct tgccggccgt acccactcca
    26401 agaacggacg ggtgccacca tgtcggcgcg cgaaaattgt ccaggagtcg caaattaagg
    26461 ttcgcagcgt atacaaagct ttttgtgacg acggggccgg ccagcgctgc gcattctatg
    26521 agcaatgcga ctacatcgct gcccgccgcg atcagcgtcc cggcattctc gcattcccgc
    26581 atgcgaatct cgtcgttccg caatcgaacg acttgaagct tccagcgccg gacttggtga
    26641 tcatcgacga acgctgtttc gataagttta caaagaaatc gattatccgc gccgaagaat
    26701 tgcaggaccg cgacacctat tttcggattg gcgctcccgc agaagaaaat gcgggctatg
    26761 aacgtcacgc ggaactcggc cgttacatcg caaagatcat tatcgaggaa aaagattggc
    26821 tacaccggtt gcgtgccgac gaagacaaag cgcgcctgga ttcgggcgtg ggcaatgatt
    26881 ggtttcttat cggaaatccg aaatccctgc acaatcgcct ctccgaggcc gcaagcgccg
    26941 ccgcagccgc ggcgggcagc aacatccaaa tcgtaccgac ggatgacgac ggtgccgtcc
    27001 gggagaagct gagcgcgatc aaatctcact ctggccgacg ggtggcaggg ttgtttcgtc
    27061 ggatccgccg cgatctggag tcgccagcag agtcggttgc ggtcttaacg gaagtgagca
    27121 atggcgaaac cgtgatcgtc tgccatggct tgagggaact cgcgttttcg gagtcgacac
    27181 cgctgctcat tatagacgcg gatgcgagcg agcggattaa caatagagtt ttttcccgca
    27241 gcgaccggag cgagaaacta aggcacgtcg acgtcccggt gcgtcgccac gcgaagttca
    27301 ttcagtgtga tagctcggtt ttctcgaaat ctggatttca tggtaagggt gaggaaaaat
    27361 caaactcgat tggaacgagg ggcaaagagc aaatagaccg catcgcggcc ttcgcaaaaa
    27421 aagtcgcagg agatcacaag cgactcctaa tgtccgccaa caaggaaatt cgctgcgcgg
    27481 gcaccggtga gccttgcggc gcctggtttt ttcgaacttt gcccgcgcgt tgagatcacg
    27541 catttcggcg cgttcaccgg ccaaaaccgc tggtcacgct ttgacgtggg gatcatcatt
    27601 gggcgcgagc agccgccacc ggagtcaatc gagtgcctcg cccgggcgct ctatgctgat
    27661 gccccgcggc cgacgctgaa cttcggcggg gaatatgtca cggcgacgcg gcaatacgag
    27721 cccggcggca cccgctgggc ggactttgat gcgcatcccg atcctgcggt caatgagata
    27781 tggagatagt tcgggaacgc caaacccttc aggccatcga ccgtctgcgg ctgctgtgcc
    27841 ccgacggtcg cgaccctgag gtctacattt tgtgcaaaat tccactgccg ggcatcagcc
    27901 cagaccgcct tgaatcggcg aaagagattt tcgctggcgg taaaaagatc gaccaagcgc
    27961 tggctggcgg gatttttagc actaacccca acatcttggc ccattgtcat cccgacctgt
    28021 gggacacgcc gaaggcggca gaaaggtggt tagagtcaca taaatcagaa acccccaatt
    28081 tgcatataga tactcttctt attgcaaaat gggggtttct gaatcggctt gaattccggg
    28141 taaaaaagcc gggctcgcgg cgctggtcga tcgtcgaaat cgatgtcgca aaatacccgg
    28201 atccggaaaa gattgtgcgc gccgcgcttc gcgtgcccgc cgccgtggaa atcgaatttc
    28261 ggggaccggc atgggatgaa atcaggaagc gggccgaaaa cccggtggca attcaaacgg
    28321 cacggaagcc gaaattgttg gtgttcattc gggcggcgga ggacgcagag ctgctgcgcc
    28381 gcattcgcgg tcggccccct acccaattcg cggcctaatg accagcgcgc agatgtttcg
    28441 cgcggctggg ccctcggctg agatgcggga tttcgctgcc atattgagac ggcaggagcg
    28501 gtggcactgc catttgcgtc caaacgcggt cgctccgatg tcgaagggaa cgacgatcga
    28561 acgggcagag gtcggcgtaa cggccgagaa tcgaaatgcg gctttgcgtc ctggcagtgt
    28621 gacggcacac tcttcgggaa attgaaatgc cgagggtact tccaccatgg ccgccacgaa
    28681 aaatgcctat gacgccttag tcgaagaaat ccgcaacggg tatttcgacg atggggagcc
    28741 tgtgatgaaa tccctgcgtg tcattaccag cgacggctcc cgcgttgagg cgctcgatgc
    28801 aagcgagcac gtggcggagc tgctcgcgag gatttctgat ggcgaagagg ccttggttaa
    28861 ggccctcgcg cccatcgtcg acttgctgaa atctacgacc ggcaaggttg ccaagatcgc
    28921 gaaggcgcgc cgcgccgcag ccccgagcat gccggctgac gagtttttct cgcgcgcgct
    28981 ggcggcccaa gcggccggta aagtcagcgg gctggatatc gcgaaagccg aggcctgcct
    29041 cgcgcgcgga cttcgcctgc cggacgagat cgtcgagaag gttctcggcg atgaaacgta
    29101 caaggctctg ccggcggaga tgagttatca ggccttcatg gaccgcgccg aagctgcgtt
    29161 ggcagccggc aaggtcacgc atttggaatt ctcacgcgcc caagccgcgc tgatgaatgg
    29221 ccgccagcct gcgcccgcgt tcctttctcg gctgctgttt ggccactcgg gaacgctcgc
    29281 ggcgtgaacc gccagcgcgc acgaaaacgc gctcgccgca gcatcagtca cgctgtcaga
    29341 aaataaatcg cggacgtgct tgactcgttg tgtcggtcga cgttatcggt cgtccatcgg
    29401 ggctgcgatg ctccgaagtc gggcaggacc ggtccgagca tgtcgggggc agacgacgtg
    29461 ttcggcgaac gccgcaagtc gtgatcgaaa accgtcgggg gttacctcgg cggtttttct
    29521 gtttcggatc gatcgcgaac ataaaaatat cgaatggcga attttttctt gcgcttttcg
    29581 atgggtccgt atacggatta tcccgtcggg gcagcgacac ttgagagggg gactggggcc
    29641 aaggcggttc cagtcccttt cgtttttggg cgcaatccga ccgcacatca cttgagttag
    29701 gagaactgtg agcgattacg acgcgtgggc tgggcggcta cgccagccgc attttgactg
    29761 ggccgatgcg acccaagagc tgaaatcaga cgatatcgaa cagttgaccg aatctttcct
    29821 cgacagcgcc acgttcaacg ccgtgcgcac gcgtgtccga gaagggtttg tcccatctcg
    29881 ccagcttgac tggggatcct gggccgtccc agcgacaaag gatgacattc tggcgcacat
    29941 ggcgagatgg cagccgggct gggacttgcc ggagccggga gatgagaccc acaaaccgcg
    30001 attgcagcgg gcccgctgtt tgcaggcggt cgaagcgctg ccggcggatg gcgaatacgt
    30061 cctcgtaatc caagaatctt gaccactcgc gcgttgcagc cgtcaaaaca acgcgtgcgg
    30121 gcactgaagt gagctacagc cttgcaacca agtttctgac cgccgtggcc gtccattgct
    30181 tccccgtgcg ggtcatgatg ccggtttcat tcatcctggc ggccatggcg ttcaacgaca
    30241 aattctcggc ctgcatcgca tcgatggttt cccggtagcg ttccgctgct acatcggcgg
    30301 ccttacgcgc tgcctgcgcg cccttgcgct ggaatctggc aatgtccggt gcgttctcgc
    30361 gatagccgcc cagtaccgtc ccacgcgcct ttgcagcggc caacgcgtcc ttggtcctcc
    30421 gggagataac ctctcgctcg tgctgggcca tcgacgccat gacgcccagt gtcagcgtat
    30481 ttgcgtccgg taggtcggcg gctacaaact ttgcgccttc atccctcaac ctcatgagga
    30541 acgctacgtt gcgcgacaaa cggtccagct tcgcaattac aagcgtggcc cctgtgatcc
    30601 ggcatcgctt caatgccttg tccagttctg gcctagcatc gtccttgccc gtctccacct
    30661 ccacaaactc aggcgcaatg atcttgcccc cacggccaac cacaaattgc ctcacggctt
    30721 cccgctgggc ttcaaggccc aggccagagc gcccttgctt ggccgttgag acgcgaagat
    30781 aggccacgaa cttgctcgat tgcgcttgca tgtaatgtct ctctacgaga gttgagagtg
    30841 tttacatggg ttagtgggca cttcccatgt ggcggattgc ccccatcaac gaatcaaggc
    30901 tgcaagccgc gtgtagccgg cgcgaattcg cggacggccg cgtgaaggcg cgccggcaaa
    30961 ggccggggaa gtgatcccga ttggaaaggc ccgggcgacg caatcgggct agttcgcatg
    31021 tttcacgcaa accacaagcg tgtcttacga attgcgcatg tcattcccga taaagaagcg
    31081 gtcagttacg cctcagcaaa cgcctcggaa ggttgaacgc cacggcgaaa aacatggcga
    31141 aaagaaaaat ggtgacaagg ccggtcccaa taagcagccg ctgaaccagc ggtgtgtaga
    31201 tcccgtgcgc tgcatcaaag ccatagcaaa tcgatcgtat acggtcgcct atcgtaccga
    31261 tccgcccctc gcccgcgtcg atgagggcaa gacgcaattc ggtggggtcg agggccaggc
    31321 tggagaggcc acgcacgatg cgtccgtccc gggctatggc gagaacgatc gcaggatgcg
    31381 caaactggtc agccgcagcg tcatagacgg cgcgataacc gatatcgcgc aggaacattc
    31441 gtgtggtcgg agcgtcaccc aacagcaccg cactcgccgc ggtaatctcc tggcgatgaa
    31501 tttggcctat cgccatgcgc cgcgcatcat ccggccggtc gttggaatcg atgccgatga
    31561 cgatgagtcg aaaatcactg ttgggcttca aaccggagtc ggcgagtgca tcggaagtaa
    31621 gcgtcaatgc ggtcccgcac gtcgatttgc aggtgtagtc aacgggaatg accaacgccg
    31681 ggagaccttg cagcgcctcg gcgaggctca cggacctgcc ggcatcgtcc cgaaatgata
    31741 ggctgagcgg caaccgcgct tcgttcggca acgataaacc tacgtgcgaa agctccgttt
    31801 cactgagttc cgcatgagct gagaccgcga gcatgatgga gacagcgaga acaggtaggg
    31861 aagtccattt catggcgtgg cttctcgaga aaactggtgt gcggaccagg caagcgctct
    31921 ttcgaaaata agcaaccatg tgccccccac cccgcatatg gccgccggaa tgacgatcca
    31981 gagaatccaa ttcgaggttc cggttgccgg aaatattagc caaatcgcct caaggtagcg
    32041 caaagcgagc gtcacgcaag cgattgtgag aataggtgtg cggcccgtgc ggactcgcat
    32101 tgatagaagc gcgaaaaatg gaacgacgaa ttgcagaatg gccagtgtcc agagcaccgc
    32161 gtcccagggc gcctcagcac gccgaagata ccagagggct tcatcgggaa tgtttgccga
    32221 ccatatgatg atgtactgca tcgcctgtag ataggcccac aaaagtagcg tcgaaagcaa
    32281 tgtcgcgccg taggcatgcg tgctctcagg tttctggtgc cacagcgtca tgactaaggc
    32341 aaagcagagg cccgctagga gttgggatgc aaggaagatg aggccgaatt cggatgaatg
    32401 gaaggacggc tcgagcgatt cgatccaatc gatacctgcg aatgaagcga cgagcgcata
    32461 gacaatgagc ccaatggagg ccacccgtat catctccgca agcttcttcc acgcgcgcgc
    32521 ggcccaaaac gcaatcgcgg aaagactcgc caaatagata agcgtacgga ggatatagaa
    32581 ccacggcgtc agataaatca ccttgagcgg tccgggctca acacgcgtcg cccagggata
    32641 aagccatgac agtccaacga agacgggaat aaacaacagt ccggcccctg gaatcgtcaa
    32701 ggatgctgcc gcaagcggcg catgcattcc ctccgtccag gcggcacgta caagataggt
    32761 ggtcaacagc acggccagtg cgcccatcgg gatggacgcc accgtgacgc acgctgctag
    32821 ataagcactc aatgccgctt tcgtatcgac aaacaaacca acgccgcaaa gtgccaagcc
    32881 cgtgacagct atggccaaca ggatggctct tggcgtcatg gctgcgcctc agtgacaagc
    32941 ttgcctcttt ccgcaggggg cgcgtcgtcg agtgaggcgt tctcgctcag ctgcagcgcg
    33001 cggatatagg caacgatcgc ccaccggtcc gccggcatca cgcgatcggc gtaagaatac
    33061 atgacgccat acccattgct gatgacgtcg tagaaatgct gatccgatgc ggctcgaagc
    33121 ctctcctgat gataggttgg aggcgccgga aacccgcgcg cgacgatcat cccgcggccg
    33181 tcgccgaggc gcccgtgaca gggggagcag aatacgtcga attcatgctg tccacgctcc
    33241 agcaatgcaa gtgtcgggat tggcttttcc gccgcctgtt ttgttcgcgc cgcgtcgcca
    33301 cgtgcgacag agcccagagg cggcctctgc aggatgcggt tgtttggaaa aagttctgcc
    33361 ggcttgtact cgttgtcctt gggctgcgaa atcatgtctt gattgcaacc gcagagcgga
    33421 atcagaagaa tgaccggaat aagaaatcgg atcattccgg caccagctcc acggcgtccg
    33481 ccttcagatt ccgcatgaat tcggccgtct ttgcggaatc aaagctggga tcatgcgcgc
    33541 cgatgcaaag gaaaaagcgg tcactggagg cgcggccaaa actgggcgca ttgaacaccg
    33601 gatgataaag cttcggaagt ccgttcaatg cgagcattcc caacgcgggg aaaagtgccg
    33661 cgaacaacac cgtcagctca aacgctacaa ccgcgaaggc ggaccaggcg tattgcgcgc
    33721 gcccccctac gttgagtgga tagtcgaaat tgacgtagac ctgcatcgcc aacgcgactc
    33781 ctgcgccaaa aaacccgccg gtcaggccca gccaaaacac gcgattgtcg tttagctcca
    33841 agatgccgtt cagttcctcc accggaaagg gtgtgaaggc gtcgagatgg cagtagccct
    33901 cctgcttcgc gagccgcgta gccgcgacga gttcttccgg gctcttgaac gcgccgatga
    33961 tcccgtaaag ggcggaatta tccggcatga ctgccctcct tgtgttcttc gtgcagcacc
    34021 tctttggttt caaatatgga gatcaccgga aagacccgga taatcaacag gaatggaacg
    34081 aggaatactc ctatggtgcc gaaataggtt gcccaatccc agaagcttgc tgtgtaaagc
    34141 ccccatgacg aaacaaggaa atcgcgataa aggctggtga cgagaatcat gtagcgttcg
    34201 agccacatgc cgatcacgac cgaggttgcg atcgtaaaga atgtccaggg cgtgcgacgg
    34261 accgaacgcc accaaaggcc ctgcagcacg atgaagttga aaaaaaccgc gccccaatag
    34321 ctccatgcgt aggtgccaga aaacctatcc tggagtgtct gaagatcgtg aacattgctc
    34381 gaatacagag catcgaatat ttcaaaaaca tacccatatg ccgtcatgaa tcccgttgcg
    34441 agcagcactt taccgaggac gtcaagatgg tcgtcggtca ctagattttg cagattaaag
    34501 agcgcgcgga tcgctatcgc gattatggcg accatcgcaa aaccggagaa ggctgccccc
    34561 agcacaaagt aaggcgggaa aatcgtggaa tgccagccgg gaatttggct tgaggcgaat
    34621 agcaacgata cttcgctgtg caccgagaca accaagggca ctgcaagtgc tgcgatcagc
    34681 cggtagcatt gacgccagcg catccaatgt attgccgatc cgcgccatcc aagcgatgtc
    34741 acgccgaaaa atacctgcca gccgcgcttt cttgcacggt cgcgcgcggc gccgagatcg
    34801 gggatgagcc cgatgtacca aaaaatgacc gaaacggtga ggtaggtgag aacggcccag
    34861 atgtcccatt caagcgggct gcggaactgg ggccataccc ccatggtgct gggataggga
    34921 aacatccaat aaagaaacca cggacgaccc aaatgcagga caggatagag accagcgcag
    34981 acaacggcga acaatgtcat tgtctcggcg aagcgattta gcgaattacg ccagtcgcgg
    35041 cccataagaa gaagcagcgc agagatcaaa gttcctgcat gcccgatgcc cagccaccag
    35101 acataattgt gaatggcgaa ggcccaatta actggaatat tcaagcccca cacgcccact
    35161 ccgcgcacga acaacaccgt ggcggatacg aggaaaagcg taaaaagtgc gaatccgaga
    35221 cagagcgcta tgagccaacg gctgcgggtc ggaaaatgga gcggaattcg cgcaatctgg
    35281 tcggtaacgt caccgagatc ctgatcgggc ggaagaacgg ggcgagctgc atcggatgca
    35341 aacgacatta gctttcctcg ccctcgtcct tccactgcgc gagatacgtc gtgcgcgggc
    35401 gcgttcccaa ttcctcgagc agggcataat gacgcccgct ttgccggcgt ttggcgactt
    35461 cagaatcggc gttatttaga ttgccaaaga cgatggcttt ggtcgggcat gcctgttggc
    35521 aggcggtgac gacttcgccg ttttttatgg cgcgattttc tttatcggcg gttgcgtgtg
    35581 ccccttggat acgctgtatg caataggtgc atttctccat tacgccacgg gcacgcacgg
    35641 taacgtcggg attgtgcgcc gcaatggtgg atggatcgtc gccgcccgtg taatcgtacc
    35701 agttgaagcg ccgcaccttg tatgggcaat agctggagca ggtgcgggtg ccaatgcagc
    35761 gattgtaaac ctgctgattt acaccttcgt cgctgtgcac ggtcgcgttc accgggcatc
    35821 ccatttcgca cggcgccttt tcgcagtgca tgcagggcac gggctgaaaa tagctttgcg
    35881 gatcctcgac atcgccggtg taatagcgat cgacacggat ccattgcatt tcgcggccca
    35941 tggcgacctg ctctcggcca acgatcgcaa cattgttctc tgccgtacac gcggtgacgc
    36001 aggcattgca gccgatgcaa agatcaagat cgataaccat cgcccaggcg acgtcggttc
    36061 ctccccattc cggatagaag ctcggttgcg caggtggttt ggccaacgct gggcgcgcgg
    36121 ccgtcacctc gcgcacgagg tcgaaaccgt ccatacggtg atgaagctgt gtgctcgcga
    36181 gcctgtgctc acccttggcg cggcgcaatg tgccgacggc atgccatgcg tttgacgcgg
    36241 ggcgaaccgc ataagcgttg tagccgatgt cgccggcaat atgaccgccg tttttacggc
    36301 cgtagcccaa aaacagtgtg acagtgttgg atgcctgccc cggcatgatc cagacaggac
    36361 ctatgactgt gcgattgcca acagcgattt cgacattatc gccattggca agaaccacgc
    36421 gttccgccag cgcggggctc accagaacga cattgtccca ggtgatcttt gttaggggct
    36481 tgggcaattc ctggagccag ccgatgttgc caaaacgccc atcccagacg ctcggatcgg
    36541 gacgaaatac gatgtcgagc tgattgttca gggattgggt gacaatggtg ccagtgttgg
    36601 ccgccggaaa gggcgtatcc atctcaacag cagtgccgga aatgaaacct gcgtccagag
    36661 catccagcca gcgcggcccg agcggatcgc ctagccgatc cttccaggtg agacgcacag
    36721 ccgtggctgc atcggggtcg acgactccca gaagcatgtc aagcgcctga tgcaccgata
    36781 tcacattgta gaatggttcg atcaccggct gcaccagcgt gaccgtgcca tcgacggctc
    36841 gcccatcgct ccaactttca agcatgtgcg ccaacgggag atgccaattc gcgaggcgtc
    36901 ccgtctcatc ggcgtgatag ccgaaatgga tccgcttatt tacgcggccc atggcatccg
    36961 caaatgccaa ttctccgggt gcggaatagg ccggattgct gtcgataacg atcaacgtat
    37021 cgacttcgcc ggccttcatt gccccgacga gagtagcgat tgtgctcgca tgctgaggca
    37081 tgaagatgaa aggttcgctg tatcgcaccg tcgcaccggc gtttccaagt ttctgattga
    37141 ccagcggcgc gagttcttga atttcgagcg gaagctgacc gccgatagtc aaaagcgagc
    37201 gaccggcgtg ctgctgaaga tcacgcgcgg cggctaccac ccaggcttgc tccttcgcgg
    37261 aaagttgcac agtgcccggg cccgtaaccc cgattgctga ggccaaggcc gccgcaatat
    37321 cgggcaagcg cgaagcatcg actggaattc gcctatccgc gacggagccc gtgagcgagg
    37381 gcgtggattc ggccatatac atacgcatgc gctccgcgcc ggggcgctct tcgccgcgcc
    37441 gcgaagacca agcggacgcg ttgcttactt gttgcgggcc aggccccagc acatcgtctt
    37501 cgatgccgac aaggacatcg cattgagaaa gatcgtgatg aaggtgcatg ggcccgcctc
    37561 gtgcatcgga agcgggttcg aagagataca gtgctgcctg aggaaactca gccagaaatt
    37621 gcgtccattg ccttattaac gtagctgacg tcgtgtgccc cattaggaca tggacacctt
    37681 tgccgccttg cgatttccag tggtccctaa aagatgcgat ggcctgctgg aagtccgccc
    37741 atgttgccgg tacgccatcg cggagcggta tgttggaacg gtccggatcg taaagcccca
    37801 agatcgcggc ttgggtgaag acatcgctcg cgctcgatcc gatcgggtgt tgggcgttac
    37861 cgtcaagctt agtcggccgc ccatcatagc tcgtcgcgat cgcgggctgg gcgtaaccac
    37921 caaacattgt tgccgtcgcg taataacgcg gcacacccgg cacgagatat tccggctgct
    37981 cgacaaaggg cgcagccaaa tcggcgcggc tcctctcgct gcatccggcg aggcctgcaa
    38041 gcgcgaatga tgctgccatg aggcgcagga actcgcgccg tccgttttgc ggtacttcgg
    38101 ctgccgccgg aaattccgcc gtaagccatt cccgaaaatc gggttcgtcg gcaacctctt
    38161 ccagacctgc ccagaaggtg cggccacgcg tgccgtgtaa tttttgacgg attttctcaa
    38221 gctgcaagga tggggtgcgt gcagttttca tcggtggcac accgaacaat cggttagttc
    38281 gccgacgcgg atgtgatttt gcacgagcag actgctgcca agcgcagcga gatctccacg
    38341 cggccgccaa ttcatgtggg taatttcatt cgccggtcgc agtgcgggcg cggggttgcg
    38401 gtggcaagag atgcagaact gcatcgtcag tggaaccgct ttgtaggtca gcgccatctc
    38461 atcgatgcgg ccgtgacaag aaacgcaacc cacgcccttg gcgatatgca cgtcgtgccg
    38521 gaaatagaca tagtcgggca agcgcgccac gcgctgccaa acgagggagc gatcctggct
    38581 gaggctctcg cggacgggcg ccaacatcgg tgcacctgtc catatctgcg agtggcaggt
    38641 catgcagata tgggtggccg gaatgcctgc gcgaggtccg ttagcgacgg aggtatggca
    38701 atacacgcag tccagcccca gccctgcgac atggtgtttg tggctaaagg ggacgggctg
    38761 aggaggggga tgcaaattcg cgcccgtctc gaagtctgat cgtgcccagg caatcgcaag
    38821 cgtcacgctc gcaagcgcaa aacacacgcc gctaacaaca gcgaagcgaa tccatagatc
    38881 ggcggcaggc gtgaagattc gggacatgcg ggtgaccgca gagattggcg cattaacgcg
    38941 ggttcgagcc aaaagttccg ctatgcgccg gaacgtccgg ccacgttttg cgtttcttga
    39001 tcagctcaaa aggggggtca catgctgcaa gctctcaccc ctgtcttaaa acctgccgca
    39061 aacgaaccca cggacattag cggcctcctg atggcttatc tggcgatcag tctcatcggt
    39121 accgtggtgg gattatcgct tctattgcgc atcatattcc ccagcgcccg agatgcaccg
    39181 cctatttcaa gcttggccga tttaggcgga ctgctacagc ttcaaatcga cgaagcggga
    39241 gattttcaga aattgagacg cgcagaagac gaaagcctga attcgtacgg atggataaat
    39301 cgtaatgata agcacatccg cattccaatt aggcgcgcaa tggagctgac agccaagcgt
    39361 gggattcctg gttggccgac aaggcgatga gtagcgcatt cgtgcggtaa attgctttgc
    39421 ctgaaaaata acgtggcggc caggatgcag tggattcctt ttttcacgcc gggcgattcg
    39481 caatactcga acgacgtcga tttgcttttc gtcggtcttt tgactgcaag cgtgttgatg
    39541 ctagtgcttt tgttcggcct gatgctgatt tttatcgtgc gatttcgcga gaatagcggc
    39601 gtaaaacggc ctcacccagc cgaaaagagt tggcgctggg aaattgcgtg gacgagcgcg
    39661 accgcattga tcttcgtcat catgttcgca tggggcgcat cgctctatgt gcgcatttat
    39721 cgcccgcccg acgacgcgct tacgatcaac gtggtggcca agcaatggat gtggaaagcc
    39781 gagcatcccg gtggccaaat ggagatcaat gcgctccacg ttccagttgg acgtgcgatc
    39841 cggcttatca tggcttcgca agatgtgatc catagcttct tcgtgcccgc gtttcgctta
    39901 aaacacgatg tcgtgcccgg ccggttccaa tcgctctggt tcactgcgct acggcctggg
    39961 acattccagc ttttctgcgc cgaatattgc ggcacggacc attcgcggat gaccggaagc
    40021 atcgtcgtgc ttcggcccgc cgaatatcag gactggctgc gggatcaggt cggtggcgac
    40081 accctggcag cggagggtgc aagtctcttt cgctccttgg gatgcagcgg ctgccatgcc
    40141 ccaggcagca cagtccacgc gccacggctc gaaggcgtct atggcaatcc cgtaccgttg
    40201 tcagatggga ccatagtgcc ggcggacgac cgctatatcc gcgattccat cttgcagcct
    40261 cgatcccaga tcgctgccgg ctacgagcca atcatgccgg tctttgctgg ccaaatctct
    40321 gaagacgagt tgttgcgtct tgtcgcctac atcaagtcgc tcgccaacgc gaagagaggg
    40381 gcacaatgaa cgcgcacgcg ccagtcaatt atctcaccac ggactaccgc ctttcgtcct
    40441 ggttcaccac caccgaccac aaacggatcg ccattctcta cttcgcaacg atcacgttgt
    40501 tctttttcat cggcggcatg gccgcgacga tcatacgtct cgaattggcg acgccggcgg
    40561 gcgatttggt tacctcggac gtctacaacc gcctttttac aattcacggc atcgtcatgg
    40621 tgtggttatt tctcgtgccg tcgatcccga cgactctggg caatttcctc gtccccctga
    40681 tgatcggggc ccgtgatctg gcttttccga agctcaatct gctcagctgg tatttgtttg
    40741 ttgctggtgg agcgtgtgtt ctttatgcgc ttttcgtcgg cggactggat acaggctgga
    40801 cattctacac gccgttctcc tcgacctatt ccaaaggaaa tgtgttcgtg gccgtcctcg
    40861 gcattttcat agccggattg tcttccatcc taacagggct gaatttcatc gtcacgatcc
    40921 atcgtctacg ggcaccgggc ctcacttggt tccgactccc gctcttcatc tgggcgatct
    40981 atgcgacagc catcattctc gttctggcta cgcccgttct cgctatgacc ttgttgctca
    41041 tgacgctcga acgggagttc ggcgttccca tctttgatcc ggcgcgcggc ggcgacccgt
    41101 tgctgtttca gcatctcttc tggttctact cgcatcctgc tgtctacatt atgatcctac
    41161 ccggcatggg cgttgtaagc gaattgatac cctgcttctc gcgcaagccg atctttggat
    41221 acaaattcgt cgcgtggtcg gcgatctcca tcgccatcgt gggctttctc gtttggggtc
    41281 atcatatgtt cgtcagcggg caatcggtct tcgctggcgt cgttttttcc ttgatgagct
    41341 atctggttgc ggtaccctcc gcgatcaagg tctttaactg gaccgcgacg ctacatggcg
    41401 gatggatcaa attagattcg ccaatgctct atgccttggg ctttatcggc ctgttcacaa
    41461 taggagggct cagcgggctt ttcctcgcct gtctcgccta cgacgtctat gtcacggaca
    41521 catatttcgt ggtggcccat ttccactaca ttatggtcgg cgccacggtg acggcctatt
    41581 tcggcgggct gcatttctgg tggccgaaga taaccggccg tctctatccg gaatattggg
    41641 cgcgcgcggc agccatcgtt attttcttcg gctttaacct aacctttttt ccgcagttca
    41701 tcctgggcgt tctcgggatg ccgcggcgct accatgttta tcccgcagaa ttccaagttt
    41761 ggaacgtgct ttcctcaggt ggcgctttcg tgctggccat cggctatctc atgccactaa
    41821 tctatctgag ttggtccata ttctggggca agcgcgcgag cgccaatcct tgggccgcta
    41881 cgggccttga gtggcagacc ctctccccac cgcccaaata caactttgcc gtcacgccga
    41941 cggttgtgcg cgagccctac tgctatacgc cggaggcgtt gccgcagaat ggttgagatg
    42001 caccccgaaa tgcagttctc cgaccttgga cagcagcagg agatcgcacg tctaggcata
    42061 tgggtgttcc tcgcaaccga aatcctattc tttggcgggc ctctgctcct ctacacggtc
    42121 tatcgcggcg cgtatgcgca agggtttgcg gcggccgcgc gccacacgga catcctgatc
    42181 ggaacggcca atacgttcat cctcttgaca agcagttttc tcgtcgcttg ggcaacagaa
    42241 gccatcaaga aggacgatgg ccgtttgacc gcgctcttgt ttggggccgc agtttttctc
    42301 ggccttgtct ttcttggcct gaaaggtatg gagtatagca acgaatatca cgaacaactc
    42361 attccgggct tcggttttgc attcgccgag gccgagcgga atgcggccct tctatttttc
    42421 acgttctatt tcatcaccac gattctgcac gcaatccacc tcatgatcgg aattgtcctg
    42481 ctcattgtaa tagcgcggca cgctgcaaag ggacgatacg gtgctggtta taatgcgcct
    42541 gtgttcgtta gtgcgcttta ctggcatttc atcgatctcg tgtggattgt cctattcgcc
    42601 ctcatctatc tgccagggcg gagcggccca tgaccgcaaa cagcagaagc tggaaaccgc
    42661 ccgcaagact tgggattgcc tggatcggtc ttgtgctttt gcttggcagt acggtcagcc
    42721 tatcctttgt accgctcggc tgggagaaca cggccatcgc attgttcata gccttcgcaa
    42781 aggtagcgat tgtcgcagtg attttcatgg aactcgataa aggcaaagct ctggtgcgaa
    42841 tcttcgcact cgccggctta gtgtggctgg cgatcatgtt ctggttcgtg ctcaacgatt
    42901 atgccacacg atccgaattt ccgccgccgg cggcggcacg gccagcacta agtgactctt
    42961 ccgatcatta gtcgtctcag cagtcatcat cacacttgtc ttgtctggaa cgaatgtgat
    43021 cgaaagccgt tcggcattcg cgcggtatac cgatggcaaa ggaaaaaaca tggcagccaa
    43081 ggacgagggt ggacatgagg tccattccga cggtgccgcg ggcggttggg gttcgctgcg
    43141 cgggataggc cgcgtgcttc tggaagagca cgatacaccg gaagtagcgg cgaccttgat
    43201 gcggcagaac aagccgggcg gcttcatgtg cgtttcctgc gcctggacca agcctgccga
    43261 ttatcatccg ttcgagttct gcgagaatgg tgccaaggct acgctgtggg agctgacgaa
    43321 acggcgctgc acgcccgaga ccctggagaa gcatacgctc gccgaattac gcgagtggaa
    43381 agaccatgac cttgagcagt tgggtcggct gacacatccg atgcgttacg acggcagaag
    43441 cgacacatat gtccgatgca actgggacga cgcctttgcc gccatcggcg ccgaactgac
    43501 agccttggat cccaaatcga ccgtcttcta cgcttcaggc cgtgccagtc tcgagacggc
    43561 gtttctgtac gctttattcg cgcggctcta cggtcacaac aatctgccag acagttccaa
    43621 tatgtgtcac gagacgactt ccgtcgcact gaaaaaggtt atcggcgccg gcgttggaac
    43681 cgtagtgctc gacgatttcg agaaatgcga cgctatcttc tttttcggtc agaacaccgg
    43741 ctctaatagc ccccggttcc tccatgatct gcaagccgca gtagaacgtg gggtaaagat
    43801 catcaccttc aacccggtgc gcgagaaggg tctggaggtt ttcatcaatc ctcagaatct
    43861 cgcacagatg ctgacgggca gcgaaaccca tatttcgtgc caatatcatc aagtaaaggc
    43921 cggcggcgac atcgccgcgc ttttgggttt gtgtaagcac gtgctggaag ccgacg
//
LOCUS       DASYEG010000007         4664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1307244, whole genome shotgun sequence.
ACCESSION   DASYEG010000007 DASYEG010000000
VERSION     DASYEG010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307244"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2041)
                     /locus_tag="VGM72_00440"
     CDS             complement(<1..2041)
                     /locus_tag="VGM72_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263757.1"
                     /translation="MRRELQRFRLSFFSIAIAALAAFGTMRPALAAAPEVFSLGQSSN
                     SGAVCQAAPRYDDPAAQVPYAKAWVITCRGWSDVPLGHVYTFVSDDPSPSAAGSLWRK
                     ALEARAACGVGKPVNIAGLSEATRMDCQSVPGKVPYSVYEVDDGSTVTVSEGMAALSD
                     LIELALKVATGASDPPAATARLQAGAQFSADAASNLAAAADAAQRSPEFLRQHAYIQN
                     QGWNFDDAERDFRLLATAENIAPSDRAYAQINWALNVSNQGRFAEAERHFSEAAVTAS
                     MLKDIRLDALTLNYRALDFLNQRKFAEAEAAANRAIALRGQIDPQAGTGNVDDGLVTT
                     DIGLQITPSLAANLNRRGNMTVFQVTTLTAAERMQVQNAQAYYVIGAARLRIGDAAGA
                     TENLQRADMILANPRLTISAAWLRARILDELSKLDLQAGRAEQARMRLLDATKIFQLR
                     RDLAVTPAEAALYLNVARIEAETGATEQALADYATGFDLFRQSRGTLGDSADETKPYF
                     DLLIAQSRSDPARARDYQRRFFDALQSLVSQASAQTIARLAARFSQSGTGAAAARALE
                     DAQRQFTIKSSEIQRRQEAGDYSAQLRDADNSELKALNDRLQGLQQQLLQANPRYGQL
                     LAATTTLDDLQKALRPGELYVKVTLLGEGGYALAVSSQDVRIYAIPLTRAQAQSQV"
     gene            complement(2038..>4664)
                     /locus_tag="VGM72_00445"
     CDS             complement(2038..>4664)
                     /locus_tag="VGM72_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263758.1"
                     /translation="HNSGGGIDASGAVIKAATLTGSAAGAVDFSNAANSFGTLGGFSN
                     TAGPTSIKDTLPLSVTGAVDATGQTLTLVDTGGGIDASTVAIKAGTLTGSTAGAANFS
                     NPGNLIAALGSFTNISGSVSIRDNASLSITGTLDATGQSLSLVDTGGGIDASGAAIKA
                     DTLTGSSAGTTNLSNTGNVIGSLNGFTGTSGSLSIANSTTLTVTGTIDATGQSLTLQS
                     SNGGIDAAGAIIKAGTLGASSAGAGDFTNPANAIGNLGNFSNTAGSLSIRDSVPLSVG
                     GTVDASGQTLTLNNSGGGIAGSGLVKAATLTGSSTGAVNLSNSGNLIGTLNNFTNTSG
                     SLSIADGTSLTVTGTIDATGQTLSLSNSGGGTNAAAAVIKAATLTGSAAGTADFTNAA
                     NSFGNLGPFTNTAGPTAITDTLPLSVTGTLNATGQTLTLVDTGGGIDASGAIIKTAKL
                     TGASAGFADFSNAANSFAVLGDFTNISGPLSIRDTVPLTVSGTVNAASQVLTLNDPAG
                     AIDASGGIVKAGTLTGASAGQADFSNPANAIGTLSNFNNGSGPLSVVDSVPLTITGTV
                     NAAGQTLTFTDTGGGIEAAGGAIKAKLLTGSTKGTADFANAGNAVDTLGDFALTTGDF
                     TLNNSQALDVQGFVRTGTLDSSGGPALGSGNITLKTTSGSLTVTGADNSGGKLQANDV
                     SLTAAGNILMGSGDNGDGQGFIPLFRNLEDPAAIDLLIDTLEPAKTANVFILANSVKV
                     ESPGTILQQNTGTKTIPDGVRIFHFGASTAISISGRPLVVNMFGTLVIDGVPLAASAI
                     ASAASVRLFPPIFNNHYRVDNCIIGQGGSCTNIQFNISAIQPVGLAGLLTVRGVDDDN
                     QNDPTITGAGNDELDDQP"
BASE COUNT          871 a         1395 c         1481 g          917 t
ORIGIN      
        1 tcacttgcga ctgcgcctga gcccgcgtga gggggatcgc atagatgcga acatcctggc
       61 tggagacggc caacgcataa cccccttccc ccagaagggt gaccttgacg tagagttcgc
      121 cgggacgaag cgccttttgc aggtcgtcga gtgtggttgt tgcggccaga agctgcccgt
      181 agcggggatt ggcttgaagc aattgttgct gcaggccctg cagccgatca ttgagcgcct
      241 tcagctcgga attgtcggca tcgcgaagct gcgcggaata atcgcccgct tcctgacggc
      301 gctggatctc ggaggatttg atggtgaact gacgctgcgc atcttcgagt gcgcgcgctg
      361 cagcggcccc ggtaccggat tggctgaaac gtgccgccag ccgcgcgatg gtctgtgcgc
      421 tggcctggct gacgagggac tgcagggcgt cgaagaagcg gcgctggtaa tcgcgagcgc
      481 gcgccgggtc cgatcgggac tgggcaatca agagatcgaa atacggcttc gtttcatcgg
      541 cagaatcgcc caaggtcccg cgcgattgcc ggaacagatc gaacccggtc gcataatcgg
      601 ctagcgcctg ctccgtggcg ccggtttcgg cctcgatccg cgccacattc agataaagcg
      661 cggcttccgc aggcgtgacc gcaaggtcgc gccgcagctg gaagatcttc gttgcatcca
      721 gcaagcgcat acgggcctgc tcggcgcggc ctgcctgcaa atcgagcttg ctcaattcgt
      781 ccagaatgcg ggcacgcaac cacgccgccg aaatcgtcag acgaggattg gcgaggatca
      841 tatccgcgcg ttgcagattt tctgtggctc cggccgcatc gccgatgcgc agacgcgccg
      901 cgccgatgac gtaataggct tgggcattct gcacctgcat gcgttcggcc gccgtcagag
      961 tggtgacttg aaagacggtc atgttgccgc gccggttcag attcgccgcg agcgacggcg
     1021 tgatctgcaa tccgatatcc gtggtgacca gcccatcgtc cacattgccg gtgcccgcct
     1081 gcggatcgat ttgaccgcgc agcgcaatgg cccggtttgc cgccgcttcc gcttcggcga
     1141 atttgcgctg attgagaaaa tccagggcgc gataattcaa tgtcaacgca tcgagccgaa
     1201 tatccttgag catcgatgcg gtcacggccg cctcggaaaa atggcgctcg gcttccgcga
     1261 aacggccttg attggaaaca ttcagggccc agttgatctg agcataggct ctgtcactcg
     1321 gggcgatgtt ctctgctgtg gcaagcagcc gaaaatcgcg ctccgcgtcg tcgaaattcc
     1381 aaccctgatt ctggatgtac gcgtgctgcc gcaggaactc cggcgaacgc tgcgcggcat
     1441 ccgccgccgc ggcgagattg ctggcggcat ccgccgagaa ctgcgcgccg gcctgaagcc
     1501 gcgcagtggc tgccggagga tcggaagcgc cggttgcgac tttgagcgcc agttcaatga
     1561 gatcggacag cgccgccatg ccttccgaca cggtgacggt gctgccatcg tccacttcat
     1621 aaacggagta gggcacctta ccgggcaccg attggcagtc catacgggtg gcctcggaaa
     1681 gcccggcaat gtttaccggc tttcccacgc cgcacgctgc acgcgcctcc agcgctttgc
     1741 gccaaagact ccccgctgcg gacggtgatg gatcgtctga aacgaaggta tagacatggc
     1801 ccagtggaac atcggaccat ccacggcagg tgataaccca cgctttcgca tagggcactt
     1861 gcgcggcggg atcgtcatag cggggcgcag cctggcagac cgcgcccgag ttgctgctct
     1921 ggcccagcga aaacacctcc ggcgccgcgg ccaatgccgg tcgcattgtg ccgaaagctg
     1981 caagcgcagc aatggcaatg gaaaagaagg atagcctgaa gcgttggagt tctcgtctca
     2041 tggctggtca tccaactcgt cattgcctgc gccagttatc gtcggatcgt tctgattgtc
     2101 atcgtccaca cccctgaccg taagcaggcc cgcgagcccc acgggttgaa tcgcactgat
     2161 attgaactgg atattcgtgc aactgccgcc ctggccgatg atgcaattgt ccacgcgata
     2221 atgattgttg aaaatcgggg gaaagagccg gaccgatgcg gcgcttgcta tggcgctggc
     2281 cgcaagaggt acgccatcga ttacgagagt gccgaacata ttgaccacaa gcgggcgtcc
     2341 gctaatcgaa attgccgtcg aggcgccgaa atgaaagata cgaacgccat cgggaatggt
     2401 cttcgtacct gtgttctgct ggagaatcgt ccccggcgat tccactttca cgctgttagc
     2461 gaggatgaag acattcgcgg ttttggcggg ctccagcgta tcgatcagca gatcgatcgc
     2521 ggccggatcc tcaaggttgc gaaacagcgg tatgaaacct tggccatcgc cattgtcgcc
     2581 ggagcccatg aggatatttc cagccgcggt gaggctcaca tcgttggcct gaagttttcc
     2641 gccactgttg tccgcgcccg ttaccgtcag actgcccgaa gtcgtcttca gcgtgatgtt
     2701 ccccgagccc aaggcggggc cgccgctact atccaacgta ccggttcgca cgaacccctg
     2761 aacatccagg gcctgagaat tgttcagcgt gaaatcgccg gtggtgagcg cgaaatcgcc
     2821 cagcgtgtcg acggcattgc ccgcgtttgc gaaatccgca gtccccttcg tcgaaccggt
     2881 gagcaatttc gccttgatcg cgccacccgc cgcttcgatg ccgccgcccg tgtcagtgaa
     2941 cgtcaaggtc tgacccgcgg cgttcaccgt accggtgatg gtcagcggca cgctgtccac
     3001 gacgctcagc ggtcccgaac cgttgttgaa attgctcagc gtaccgatgg cattcgcagg
     3061 gttcgagaaa tcggcttgtc ccgccgatgc cccggtaagg gtgccggctt tgacgattcc
     3121 gcccgacgcg tcgatggcac cggccgggtc attcagtgtg agaacctggc tcgccgcgtt
     3181 cacggttccg gaaaccgtca atgggacggt atcgcggatc gagagcggac cggaaatgtt
     3241 ggtgaagtcg cccaatacgg caaaggaatt tgccgcattg gagaaatccg caaagccggc
     3301 ggaagcaccg gtcagctttg ccgttttgat gatggcaccg ctggcgtcga ttccgccgcc
     3361 ggtatcgaca agggtgaggg tctggccggt ggcgttcagg gtgccggtga ccgagagggg
     3421 cagggtgtcg gtgatcgcgg ttggccccgc ggtattggtg aaggggccga gattgccaaa
     3481 gctgttggcg gcgttggtga aatcggccgt gccggcggcg gagccggtca atgtggctgc
     3541 cttgatcacg gcggcggctg cgttcgtacc gccgccgcta ttgctcaggg aaagcgtctg
     3601 acccgtggca tcgatcgtgc cggtgacggt caaggatgtg ccgtccgcga tcgacaacga
     3661 acctgaagtg ttggtgaagt tgttgagcgt gccgatgagg ttgccgctat tcgacaaatt
     3721 gacggcaccg gtggacgagc ccgtcagtgt tgcggccttt acaaggcccg atccggcaat
     3781 tccgccgccg ctgttgttga gcgtcagcgt ctgacccgag gcatcgacgg ttccgccaac
     3841 cgatagcggc acggaatccc ggatcgaaag ggaccctgcc gtgttgctga aattgcccag
     3901 attcccgatg gcgtttgccg gattggtaaa atctcccgcg cccgccgaag acgctcccag
     3961 cgttccggct ttgatgatcg cacccgccgc atcgattccg ccattgctgc tttgaagcgt
     4021 caaactctga ccggtcgcat cgatcgtgcc ggtgacggtc agcgttgtgc tgttggcaat
     4081 cgatagcgaa ccggacgtgc cggtgaaacc gttcagcgat ccgatgacgt ttcccgtatt
     4141 cgacagattt gtcgtcccgg ccgaggagcc ggtcagcgta tcggctttga tcgccgcacc
     4201 cgatgcatcg ataccgccgc cggtgtcgac caggctcaga ctttggcccg tggcatcgag
     4261 cgtgccggtg atgctcagcg acgcgttgtc gcggatcgac acggagccgg aaatattggt
     4321 gaagctgccg agtgcggcaa taagatttcc gggattggag aaattggccg cacccgccgt
     4381 ggatcccgtc agcgtccccg ctttgatcgc caccgttgag gcatcgattc cgccgccggt
     4441 atcgacaagg gtgagggtct ggccggtggc gtccacggcg ccggtgaccg agagcggcaa
     4501 ggtgtctttg atcgaggttg gccctgcggt gttgctgaaa ccgccgagag tgccaaagct
     4561 gttggcggca tttgaaaaat ccaccgcgcc ggccgcagag cctgtcagcg tagccgcctt
     4621 gatgaccgcg cccgaggcat cgatgccgcc cccggaattg tgga
//
LOCUS       DASYEG010000008        19295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2321434, whole genome shotgun sequence.
ACCESSION   DASYEG010000008 DASYEG010000000
VERSION     DASYEG010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 19295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19295
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2321434"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VGM72_00450"
     CDS             <1..340
                     /locus_tag="VGM72_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY4263759.1"
                     /translation="SMVEVLRSNFIRTARAKGLPTWRIVTHHALHAGLLPLVSYLGPA
                     IADIVSGSLVVEQIFGLPGIGRYFVQAALNRDYTLVLGVTIFYATLIIVLNLVADLLY
                     GVLDPRVRYA"
     gene            340..1278
                     /locus_tag="VGM72_00455"
     CDS             340..1278
                     /locus_tag="VGM72_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY4263760.1"
                     /translation="MFSRSASHPDAQAMQNAQGVKGRSLLSDARRRLFRNKAAVVSMV
                     LLSGIALMALFAPYLSPYAFRDIDYDVISCAPDWWPADDTICRAGGAHWFGTDIVGRD
                     LFVRVLYGARVSLAVGIVATFVSLAIGVIYGATAGFIGGRVDAVMMRVVDILYSLPFI
                     FFVIILMVIFNQNFILLFVAIGAVSWLTMARIVRGQTLSAKQKEFVEAARAAGVSNFG
                     IIRRHIVPNVVGPVIVYVTLTIPSVILAESFLSFLGLGIREPFTSWGVLISDGASTME
                     TAPWTLIFPAAFMALTLFCFNFIGDGLRDALDPKER"
     gene            1284..3101
                     /locus_tag="VGM72_00460"
     CDS             1284..3101
                     /locus_tag="VGM72_00460"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4263761.1"
                     /translation="MAAPVLAVKNLDVRFATPDGEVQAVKNVSFDVGQGEVLGIVGES
                     GSGKSQLFLAIAGLLAGNGRVTGSALYRGEELIGTRETRLNRIRGSKITMIFQDPLTS
                     LTPHMRVGEQIMESLRVHKHMSGAQATARAIETLELVRIPESRRRMRQYPHELSGGMR
                     QRVMIAMATACGPDLLIADEPTTALDVTVQAQILDIMRDLKTALNTSIVLISHDMGVI
                     AAMCNRVQVMRQGEFVEAGPAEDIFYRPQHPYTRMLLEAMPRIDRPARAGHIALKPLD
                     ATDSKPPLLTVEDVKVHFPIRVGGTILPKYKPLRAVDGVSFSLGHGETLGVVGESGCG
                     KSTLARAVIRLLPKTAGTVVWCGRNLGEAAENEIRTLRKEFQIVFQDPLASLDPRMTI
                     GESIAEPLRSLEPQTSRSAVRARVHAMMEKVGLDPAWINRYPHEFSGGQNQRVGVARA
                     MIVKPKLVICDEAVSALDVSIQAQIIDLILSLQAEFGMSLIFISHDLSVVRQVAHRVM
                     VLYLGRVVELASREAIYSDARHPYTKALISAVLIPDPKLERAKPRRALPPELPSPLDT
                     RSALTFLKSKRIDDPDAEQYKPRLIEVAPDHFVAEHDPV"
     gene            3187..4851
                     /locus_tag="VGM72_00465"
     CDS             3187..4851
                     /locus_tag="VGM72_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY4263762.1"
                     /translation="MSELRTRRAFLTSAGGAVLLAACGPGGTAQQGASGATARARPGE
                     IVLNRGNAAEPLSLDPHHSQAVWEDNIIGDLLVGLTTDTSNGTPIPGAAESWESSPDG
                     MIWTFRLRDHLWSDGMPVTAGDFVYGWRRILDPKTASPYGYFLYLIKNGEAVNSGKMP
                     PAALGVTALDDKTLLVELEHPAPYLLEYLMHMTTYPVPRHLIEAKGDSWTKPGTYVCN
                     GPFMLTEWDSNDHVTLVKNPRFYDAANVKLNRVIFYPTTDYGAALKRLRAGELDTQDR
                     MPAQQIDWLRKNMPEIVRTDPILVTEFLTCNMTRKLFADVRVREALSLALDRETIVNK
                     VEKVGDPPAYGMIPPATANYPGGVALDFKSLPMPERLKRAQELMRQAGYGPDKLLRTS
                     LLVRSAAPDRRRVPVAIQQMWTQIYVDAQILQLDASIFYNSIQTGDFDIANPGWSADF
                     NDASTFLELLRKGNPNNWARYDNPEYDRLLDAAGNETDLKKRGALLAMAEATALKDHA
                     WIPIYFWVSGELVRPYVKGWIKNGLQKHRSRWLSIDEAAREKTLHV"
     gene            complement(4875..6290)
                     /locus_tag="VGM72_00470"
     CDS             complement(4875..6290)
                     /locus_tag="VGM72_00470"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4263763.1"
                     /translation="MSLFLHASCDAGLAKAAVRDGPVFHPRLVLATTILASGLAFVDG
                     SVVNVGLPVIGEALHAPAGTLQWVINAYLLPVSALLLLGGAAGDRFGAKRLLILGIAI
                     FAFASILCAVAANPLWLLAARAGQGIGSAILLPNSLAILGEAFTGPERGRAIGIWAAI
                     GAGIGAVGPVLGGWLIDTVGWRAIFLINLPLAIAAILLALRFVPDVQRGAKHVPLDVA
                     GGLAATMALGLLTWGLTMGSGRGGWTAAAVAVFAGGLALLVGFVWLEGARRERAMMPL
                     ALFGSQSFIGLTLLTFLLYGALGGLLVLLPYVLITAAHYSATAAGAALLPFPLVIALA
                     SPLMGAAAGRTGPRWPLAIGPLVVAAGFLLILRMGNDARYWADVFPAVLVISVGMAIA
                     VAPLTTAVLGSVDAAHTGSASGFNSAMSRTGGLVATALLGAALGARGGELLTAAHGAA
                     LAGAIMAGAASASAFAWVRNP"
     gene            complement(6293..6556)
                     /locus_tag="VGM72_00475"
     CDS             complement(6293..6556)
                     /locus_tag="VGM72_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263764.1"
                     /translation="MTNEQIQEGFDVFVHDGDKAFGAVRQVSPHGRPELVVYVENAGD
                     FSIPLTAVDAIHDEKVILNCGKLEHRLRKAIGHAHEGEDPRIP"
     gene            complement(6603..9221)
                     /locus_tag="VGM72_00480"
     CDS             complement(6603..9221)
                     /locus_tag="VGM72_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY4263765.1"
                     /translation="MWTRFLERLVGFCCDYCIAVVGLGLLLGVGAGYYVSRNFAMDSD
                     AGNLIAKDVPWRRNSLAFDAAFPQRDNLTLVVIDGVTPERAEEAARILDAQMMASPAV
                     FPVVRDLQGSDFFAHNGLLYLPADAVRATTQQLIQAQPFLGPLAADPSLRGIMNGLNT
                     ALLGVENGQASLAALTPALSAFSETLEQVAAGHTAYLSWQTMITGKKPAPRETRRLIE
                     VRTALDYAALEPGAAAAAQLRAIAVERGLTPDKGVRVRLTGPVPLSDEEFATLTENAG
                     LMVVLMSGCILLMLWFAVRSAKIILAILATLAAGVVMSLAVGLAAVGSFNVISIAFIP
                     LIIGIGVDFGIQYAVRYRAERHWIADLDLALVQGVRGVGSSLTLAAIATATCFFSFVP
                     TDYRGLAQLGLIAGAGMIVIFFFSVSLLPAFLKLLHPKGEAAEIGYRALAPLDVFLVR
                     RRRAIVIGAGVAALLCAALLPFIRFDANPFHLRSPRVESMATLADLMKDPETAPDTID
                     VLTPSLDAAEAAARKLAALPQVRETRTLKSFVPDGQTEKIALIADAAMLLDTTLNPFE
                     VMAPPNTAEIVQSMDAAKEKLRALAGAKTSPADEAARRMANALDAVENGGQAALARAA
                     AAFIPSLNIELGRLRAALMPQMVTLENLPAELRQDWIAKDGRARTQVFPKAGVGDEAA
                     MNRFADAVSAMAPAASGAAISTRESGRTIVRAFIEAGALSFLAMAVLLTIAFRKALDV
                     AVALIALIFIGLLTFATCVVIGLPLNFANIIVLPLLFGVGVAFTIYFIVSWRSGGHDF
                     LQSSLARAVFFSALATSTGFGTLWLSSHPGTASMGELLMISLGWTLFTTFFVLPALLE
                     SVRPAAPARNLAAVRP"
     gene            complement(9224..9880)
                     /locus_tag="VGM72_00485"
     CDS             complement(9224..9880)
                     /locus_tag="VGM72_00485"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEY4263766.1"
                     /translation="MPAAMDENDPYESTNRAIFDFNQRLDDNIALPVAKFYHRAVPDF
                     ARTGIHNFLFNLDLPVTFGNDLLQANVTRGAQTFARFTVNSTLGIGGLVDVATRFDIP
                     SHTEDFGQTLGVYGAGEGYYLVLPVLGPAPARDLLGRIVDIFMDPFTYVGLRSKTEWM
                     AGRGFFEVLDLRERNIDTVEQIQRTSIDYYATVRNLYRQSRDAEIRNNTQAPENLPDF
                     "
     gene            10084..10701
                     /locus_tag="VGM72_00490"
     CDS             10084..10701
                     /locus_tag="VGM72_00490"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY4263767.1"
                     /translation="MSHDTLIHHLVRPCVRWIAPLRVTPNQLTTLRLLTGFSAAVLFA
                     RGHGAGFNAAGVVFLISMLFDRADGELARQTGQMSVAGYHYDIWSDCAANIAVFIGIG
                     FGLSGPETALGPANIWLGGVAGLAIAVLFWELYVAKLASVQGYAFLNGRLVVDPDDAM
                     VLIPILVWFGLAQALLMTAAIIASAVALSFAFVALRSRHNSTKAQ"
     gene            complement(10695..11141)
                     /locus_tag="VGM72_00495"
     CDS             complement(10695..11141)
                     /locus_tag="VGM72_00495"
                     /inference="COORDINATES: protein motif:HMM:NF021435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HEY4263768.1"
                     /translation="MKTWIPTAVILLSALCATMPASGAEPTQAGMLRIAVSNVRVGRG
                     HLHVAICPQALYLGDGCAYKIIVPARQGETVAMIGDVPAGTYAAQVYFDENDNDTLDR
                     SLIGIPEEGVGFSNDPSFLFRAPSFAETAFRFDGMTGSINVRLRYY"
     gene            11233..12213
                     /locus_tag="VGM72_00500"
     CDS             11233..12213
                     /locus_tag="VGM72_00500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEY4263769.1"
                     /translation="MKVWTLVSSAIGAGFTLWLLSAYGFERILSLVAMAGWAIPAVAA
                     FHVTQIVASGCGWRMATREDARRLRGRSYYLFRWIREAINNLLPAAQFGGVVVGTRLI
                     ARRGVPLPSAIAGAIVDTTLEFVSQIAFTLLGLGLLFLGPADRSATHFALVAFLVIGA
                     LGVGLLAAQWFGLAKLIELAAEKLGKAWGRPGPNATKGLHHEIMALYRAPRRILGGLF
                     FHFAAWVLGGFEIYISLSALGHGSSILACMVIEAFGQALKSAGFAVPASLGVQEGGYV
                     LAGAVFGLPPETGIALSLIKRLRDIVLGLPALAVWHRLEAEPHVADAPSP"
     gene            complement(12225..13331)
                     /locus_tag="VGM72_00505"
     CDS             complement(12225..13331)
                     /locus_tag="VGM72_00505"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF010006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEY4263770.1"
                     /translation="MSSDSAGPILLTPGPLTTRIETRRAMLRDWGSRDSAFIAMTAEL
                     RRRLLDVANGAGTHVAVPLQGSGTFIVEAAIATLMKPTDKLLVLVNGAYGERMMAIAN
                     RLGRPARALRWAEDRAVDSATVDAALGDDPAITHVALVHCETTSGLLNPLPEIANLVA
                     RHKRAFLLDAMSSFGALEIDLKTIAVTAVLASSNKCLEGVPGLGFSLIESGALTAAKG
                     VSPSLSLDLHDQWRGFEANGQWRFTPPVQVVAALVEALRRFEAEGGQPARLARYRQNF
                     SVLLEGMRRLGFALFLDEAIQAPIIATFRSPPQFPFERFYEALAARGFLIYPGKLTKA
                     ESFRIGCIGALEPGDFGRLIDAVAASLDALRAHA"
     gene            complement(13328..14506)
                     /gene="aepY"
                     /locus_tag="VGM72_00510"
     CDS             complement(13328..14506)
                     /gene="aepY"
                     /locus_tag="VGM72_00510"
                     /EC_number="4.1.1.82"
                     /inference="COORDINATES: protein motif:HMM:TIGR03297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033980 - phosphonopyruvate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HEY4263771.1"
                     /translation="MISARAFLQEATGRGFDFFTGVPCSFLTPLINGVLSDRSLSYVG
                     AASEGEAVAIASGAWLAGRRTVVMCQNSGLGNAVNPLTSLNAPFKIPTLFITTWRGEP
                     AIADEPQHEVMGRITQDLLSLIGLAQAPFPADVAAIGPALDRAVEHMDASAQPFALVM
                     RKVDVEEEPLDSAPLPSRVAGQRIRNEQAGTQPARAAVLERLLRVVDDETGIIATTGK
                     CGRELFTIADRPQHLYQVGSMGCATGMGLGVALNTGRPIVVLDGDGAALMKLGTFATV
                     GAYGPRNLIHILLDNGVHDSTGGQATVSASVDFAAVALACGYTWAASCNTLEGFESAF
                     RSALEGPKPALIHMRIAPGSMAKLGRPTVTPAEVARRFKAFLAQGPDAARVKSAERAA
                     "
     gene            complement(14562..16214)
                     /gene="aepX"
                     /locus_tag="VGM72_00515"
     CDS             complement(14562..16214)
                     /gene="aepX"
                     /locus_tag="VGM72_00515"
                     /EC_number="5.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="HEY4263772.1"
                     /translation="MNIQDRHTIALRPATERPSKAADLRRQIETPELSFLMEAHNGLS
                     AKIAQEAGFSALWASGLTISASLGLRDSNEASWTQVLDVLEFMSDATQLPILVDGDSG
                     YGNFNNVRRLVGKLCAHGIAGVCIEDKLFPKTNSFIGSAQPLADIDEFCGRIKAGKDS
                     QTDDDFVLVARVEALISGRGMDEALTRAEAYWKAGADAILIHSKLTTASEILAFAERW
                     ANRAPLVIVPTTYYATPTEHFRQARISAVIWANHALRASITAMRETARRIFEDQSLTE
                     IEGRVASVKDVFALAGNAELEAAEQRYLPDRATARAIVLAASRGGLGELTQDRPKCMI
                     DVRGHALLQRLVWTLADSGIRNVSVVCGYRKDAVTVQGISIIDNERHAETGELYSLAR
                     AGDRLDGETILVYGDVLFRQYILDELMAVKGDIVLAVDALKLFEQSGQQYRDLVAADR
                     PFSGLYLDEEPVYLRAMAPDLPKDKISGEWMGLARLSGQGAEWMREEIAAMTAEGLVE
                     GGDLPLLFNRLTAKHPVSVHYFTGHWLDVDTVQDLANARNFT"
     gene            16336..17085
                     /locus_tag="VGM72_00520"
     CDS             16336..17085
                     /locus_tag="VGM72_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HEY4263773.1"
                     /translation="MKSASPPLTAIILAAGVGRRLGAADGKPKVLLEFGGKTLLQRHL
                     EALHAHGVTDIALTVGHQAGAIRAALEALGYGARVACVENTRYREGSLVSLWAQRARL
                     QAGSEVVLMDGDVLYDSRMIGRLLGAAPENVLLVDREIEPGDEPVKICFRDGRIVDFR
                     KKPDQPHDWHGESVGFFRFSPSMAQALAGQCGIYVEEGRANVEYEEAVRDLLLREPSR
                     FGVEDISDLPWTEIDFDADVARARDEILPQL"
     gene            17094..17870
                     /locus_tag="VGM72_00525"
     CDS             17094..17870
                     /locus_tag="VGM72_00525"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY4263774.1"
                     /translation="MTQTALENPLSDADLSQYERTGWVVRRGFFDAEDAAAIARWTDE
                     VTAMPELPGRQMVYYEPSLVAPDARVIQRIENFCPYHESFDALVRRSRLTRAANALLG
                     APSCLFKEKINYKMAGGAGFEPHQDQQAGWSRYAPIFVTALVCIDRATVANGCLEMPT
                     VRRLKQMIGAEWRPLTRDEMASFELVPVETEPGDVIFFDSYVPHSSQPNLTSDARRIL
                     YLSYNRLSDGDHRLQYFAEKRANFPPDIERAPGKDYKFRV"
     gene            complement(17877..18530)
                     /locus_tag="VGM72_00530"
     CDS             complement(17877..18530)
                     /locus_tag="VGM72_00530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4263775.1"
                     /translation="MKFADNGRPMAFLRLWTALAFLLLLSPGISPARAADPAPDPAAR
                     QIEMFYSVLIDTMKQGQQLGLQGRYRQMSPAVREAFDLPAMAQLSVGPAWQMLSEADR
                     KRITDAFERMTIANYAANFAGFSGQKFTVDPMVKMRNDEKIVESKLMSGSSTVPFNYR
                     MHLAPDGKWKIVDIYLNGYVSQLAVRRSDFAATIASSGAAGLVKKIDDLVEKQMAGQ"
     gene            complement(18530..>19295)
                     /locus_tag="VGM72_00535"
     CDS             complement(18530..>19295)
                     /locus_tag="VGM72_00535"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEY4263776.1"
                     /translation="FAGGKRLRPFLVLRAAALFGVDAACALRAAAAVEVLHTYSLVHD
                     DLPCMDDDNLRRGRPTTHVAFDEATAVLAGDALLTVAFGILSNRETHPSPEVRSQLVE
                     RLARAAGHDGMIGGQIIDIEAESRPFNAEETVTLQQMKTGALLEFSCAAGPILAQAAP
                     EDHDRLHVYARDLGLVFQITDDLLDVLGSAEKTGKSVGKDKDRGKATLVSLLGIEGAR
                     NKAMILAQHAAGVLECYGDAATDLRELALFLVERDS"
BASE COUNT         3712 a         6137 c         5901 g         3545 t
ORIGIN      
        1 cagcatggtc gaagtgctgc gttccaattt catccgcacg gcacgcgcca agggccttcc
       61 cacctggcgg atcgtcaccc atcacgcgct gcacgcgggc ctgttgccgc tggtgagcta
      121 tctcggtccg gctatcgccg acatcgtgag cggttcgctc gtcgtggaac agattttcgg
      181 acttcccggc atcgggcgct atttcgtgca ggcggccctc aatcgcgatt acacgctggt
      241 cctgggtgtc acgatcttct acgcgacgct catcatcgtg ctgaacctgg ttgccgatct
      301 gctctatggc gtgctcgatc cgcgggtgag gtacgcgtga tgttttcccg ctcggcttcc
      361 catcccgatg cgcaagcgat gcagaacgcc caaggcgtca agggccgcag cctcctcagc
      421 gatgcgcgcc ggcgtctgtt ccgcaacaag gccgccgtcg tgagcatggt gctgctgagc
      481 ggaatcgcgc tgatggcatt gttcgcgccc tacctctcgc cctatgcgtt tcgcgatatc
      541 gattacgacg tgatctcgtg cgcgccggac tggtggccgg cggacgacac catctgccgg
      601 gccggcgggg cgcattggtt cggcaccgat atcgtgggcc gcgatctttt cgtgcgtgtg
      661 ctttatggcg cgcgggtctc gctcgcggtg ggcatcgtgg ccaccttcgt cagcctcgcc
      721 atcggtgtga tctatggcgc gaccgccgga ttcatcggcg gacgagtcga tgccgtgatg
      781 atgcgcgttg tcgacattct ctattcgctg cccttcatct tcttcgtcat catcctgatg
      841 gtgatcttca atcaaaattt catcctcctg tttgtcgcca tcggcgcggt ctcctggctg
      901 accatggccc gaatcgtccg tggacagacg ctttccgcca agcaaaagga attcgtcgaa
      961 gccgcgcgcg ccgccggcgt cagcaatttc ggcatcatcc gccggcatat cgtacccaat
     1021 gtggtggggc cggtgatcgt ctatgtcacg ctgaccattc cgagcgtcat cctggccgag
     1081 agcttcctgt cctttctggg gctcggcatc cgcgaacctt tcacctcctg gggcgtgttg
     1141 atttccgacg gtgcctcgac catggagacc gcgccctgga cgctgatctt tccggccgcc
     1201 ttcatggcgc tgaccctgtt ctgcttcaat ttcatcggtg acggcctgcg cgacgcgctg
     1261 gaccccaagg aacgctgaac gcgatggccg cacccgttct tgccgtgaag aacctcgatg
     1321 tgcgtttcgc cacgcccgac ggcgaggtcc aggcggtgaa gaatgtcagc ttcgatgtgg
     1381 gccaaggcga ggtcctcggc atcgtcggcg aatcgggttc gggcaaaagc cagcttttcc
     1441 tcgccatcgc gggtcttctc gcgggcaatg gccgcgtgac cggcagtgcg ctctatcgcg
     1501 gcgaggaact gatcggaacg cgcgaaaccc gcctgaaccg gatccgcggt tcgaagatca
     1561 ccatgatctt tcaggatccg ctgacctcgc tgaccccgca tatgcgcgtc ggcgaacaga
     1621 tcatggaaag cttgcgcgtg cacaagcata tgagcggcgc gcaagccaca gcccgcgcca
     1681 tcgagaccct ggaattggtg cgcattccgg aaagccggcg ccgcatgcgc caatatcccc
     1741 acgagctttc cggaggcatg cgtcagcgcg tcatgatcgc catggcgacg gcctgcgggc
     1801 ccgatctttt gatcgccgac gaacccacca ccgcgctcga tgtcacggtg caggcgcaaa
     1861 tcctcgacat catgcgcgat ttgaagaccg cgctgaacac ctcaatcgtg ctcatcagcc
     1921 atgacatggg cgtcatcgcc gcgatgtgca accgcgttca ggtgatgcgc cagggcgaat
     1981 tcgtcgaagc cggtcccgcc gaggatatct tctaccggcc ccagcatcct tatacccgga
     2041 tgctgttgga ggccatgccg cgcatcgacc ggccggcgcg cgcgggccac atcgcgctga
     2101 agccgctgga cgcgaccgat tcaaagcccc cgttgctcac ggtcgaagac gtgaaggtgc
     2161 atttcccgat ccgggtcggc gggacgatcc tgcccaaata caaacctttg cgcgcggtgg
     2221 acggcgtcag cttctcgctc gggcacggcg aaacgctggg tgtggtgggt gagtccggtt
     2281 gcggaaaatc gacgctggcg cgcgcggtga taaggctctt gcccaagacg gcgggcaccg
     2341 tcgtgtggtg cggacgcaat ctcggtgagg ccgcggaaaa cgaaatccgg accttgcgca
     2401 aggaattcca gatcgtcttc caggacccgc tcgcaagtct cgacccgcgc atgaccatcg
     2461 gcgaaagcat cgccgagcct ttgcgctcgc tcgagccgca aacctcgcgg tccgcggtgc
     2521 gcgcacgcgt gcacgcgatg atggaaaagg tcggcctcga ccccgcctgg atcaaccgct
     2581 acccgcacga attctccggt ggccagaacc agcgcgtggg tgtcgcgcgc gccatgatcg
     2641 tgaagcccaa gctcgtgatc tgcgacgagg cggtcagcgc gctcgatgtt tccattcagg
     2701 cccagatcat cgatctgatc ctcagcttgc aggccgaatt cgggatgtcg ctgatcttca
     2761 tcagccatga cctgtcggtc gtgcgccagg tcgcccatcg cgtaatggtg ctgtatctgg
     2821 gccgcgtggt ggaacttgcc tcgcgcgagg caatctattc cgacgcgcgc cacccctata
     2881 ccaaggcgct gatctccgcc gtcctgatcc ccgatcccaa actcgagcgc gccaagccca
     2941 gacgcgcgct gccgccggag ctgccctcgc cgctcgacac gcgttcggcg ctcacattcc
     3001 tcaaatccaa gcggatcgac gatcccgatg ccgagcagta caaacccagg ttgatcgaag
     3061 tggcgccgga tcatttcgtg gcggaacacg accccgtgta agcggcgctt gtttttggaa
     3121 ctgtggcgcg ccgcattgta atcttgccgc gtctgagtgc gggacattcg agtgcgcagg
     3181 acatcgatga gcgaattgag aacgaggcgc gcgtttttga cctccgccgg gggcgcggtt
     3241 ctgctggccg cctgcggacc cggcggcacg gcgcaacagg gggcaagtgg cgcaaccgcg
     3301 cgtgcgcgtc ccggcgaaat cgtgctcaat cgcggcaatg ccgccgagcc gctcagcctc
     3361 gatccgcatc attcgcaagc cgtgtgggaa gacaacatca tcggcgatct tctggtcggg
     3421 ctcaccaccg acacgtccaa cggaacgccg atcccgggcg ccgccgaaag ctgggaatcg
     3481 tcccccgacg gcatgatctg gactttccgt ctgcgcgatc atctgtggtc ggacggaatg
     3541 ccggtgacgg ccggggattt cgtctatggc tggcgccgca tcctcgatcc gaaaaccgca
     3601 tcgccctatg gctattttct ctatctgatc aagaacggcg aagcggtgaa ctccggcaag
     3661 atgccgccgg ccgcactcgg cgtcaccgcg ctcgatgaca agactctcct cgtcgaactc
     3721 gaacatcccg cgccctatct gctcgaatat ttgatgcaca tgacaaccta tccggtgccg
     3781 cgccatctga tcgaggcgaa gggcgattcc tggacgaagc ccggaaccta tgtctgcaac
     3841 ggccccttca tgctgacgga atgggattcg aacgaccatg tgacgctggt gaagaaccct
     3901 cgtttctatg atgcggcaaa tgtgaaattg aaccgggtga tcttttatcc gaccacggat
     3961 tatggcgcgg cgctgaagcg cctgcgcgcg ggcgagctcg atacgcagga ccgcatgcct
     4021 gcccagcaga tcgactggct gaggaagaac atgccggaga tcgtgcgcac cgatccgatc
     4081 ctggtgacgg aattcctgac ctgcaacatg acccgcaaac ttttcgccga tgtacgtgtg
     4141 cgcgaagccc tcagccttgc gctcgaccgc gagacgatcg tgaacaaggt cgaaaaggtc
     4201 ggcgatccgc cggcctacgg tatgattccg ccggcgaccg ccaattatcc cggtggcgtg
     4261 gccctggatt tcaagtcgct gcccatgccc gaacggctga agcgcgcgca agagctgatg
     4321 cgccaagccg gttatggtcc ggacaaactg ttgcgtacat cgcttctcgt gcgctccgcc
     4381 gcgcccgacc gccgccgcgt acccgtggcg atccagcaga tgtggacgca gatctatgtc
     4441 gatgcgcaaa tcctgcaact cgacgcatct attttctaca acagtatcca gaccggcgat
     4501 ttcgacatcg ccaatccggg ctggagcgcg gatttcaacg acgcatcgac tttcctggaa
     4561 ttgctgcgca agggcaatcc gaacaattgg gcgcgttacg acaacccgga atatgaccgg
     4621 ctgctcgatg cggcgggcaa cgaaaccgat ctcaagaaac gcggcgcgct gctggccatg
     4681 gccgaggcga ccgcgctgaa ggatcacgcc tggattccga tttatttctg ggtctctggc
     4741 gagctggtgc gcccttatgt caaaggctgg atcaaaaacg gactgcagaa acatcgttcg
     4801 cgctggctgt ccatcgatga agccgcgcgc gaaaagaccc tgcatgtgta ggctgtgacg
     4861 ggctatctcg cgccttaagg atttcggacc catgcgaagg cactcgccga cgcggcaccg
     4921 gccatgatcg cgccggcgag cgcggcaccg tgagcggccg tcagcaattc ccctcctcgc
     4981 gcgccaagcg ccgcacccag aagtgcggtg gcaacgagac ctccggtgcg tgacatggcg
     5041 ctgttgaaac ccgatgccga accggtgtga gccgcgtcga ccgaccccaa taccgccgtt
     5101 gtcagcggcg cgaccgcgat cgccatgccg accgagatca caagcacggc tggaaaaaca
     5161 tccgcccaat agcgtgcatc gttgcccatc cgcaaaatca gaagaaatcc cgccgccacc
     5221 acaagcggcc cgatcgccag cggccagcgt ggaccggtgc ggcccgcggc cgcccccata
     5281 agcggtgagg cgagcgcgat gaccaacgga aaagggagaa gcgcggcgcc ggccgcggtt
     5341 gcggaataat gcgcggccgt gatcagcacg taaggcagca gcacgagcaa tcccccgagc
     5401 gcgccgtaca gcagaaaggt cagcagcgta agaccgatga agctctgcga accaaacagc
     5461 gcgagcggca tcatggcgcg ttcgcgccgc gcgccttcca gccatacgaa ccccacgaga
     5521 agcgcaagcc cgcccgcaaa gacagcgacg gcagcggccg tccagccccc gcgcccagag
     5581 cccatcgtca gcccccaggt cagcaggccg agcgccatcg tggcggccag gccgcctgcc
     5641 acgtcgaggg gcacatgctt tgcgccgcgc tgcacatccg gaacgaaacg gagcgccagc
     5701 aggatcgcgg caatcgccaa tggcaggttg atgagaaaga tcgcgcgcca tccgaccgta
     5761 tcgatcagcc atccgcccag caccgggccc accgcgccga ttcccgcacc gatggccgcc
     5821 catatgccga tggcgcgccc gcgctccgga cccgtgaaag cttcgccaag gatcgccaga
     5881 ctgttcggca gcaggatcgc ggaaccaatc ccctgacccg cgcgagcggc aagcagccag
     5941 agcggattcg ccgcgacggc acagagaatg gaggcgaaag cgaagatcgc aatgccaaga
     6001 atcaacagcc gtttggcgcc gaatcggtcg cccgccgcac ccccgagcag caaaagcgcg
     6061 ctgaccggca gcagatatgc gttgatgacc cattgcagcg tgccggccgg cgcgtgcagg
     6121 gcttctccaa tcaccggcag cccgacattg acgaccgagc cgtcgacgaa ggcgaggccc
     6181 gaggcaagga tcgtcgttgc caaaacgagt cgcggatgaa ataccggacc gtccctgacc
     6241 gcggccttcg ccagcccggc atcgcaggac gcatgaagaa aaaggctcac cgtcagggaa
     6301 tgcgcggatc ttcgccttca tgcgcatgcc cgatagcctt gcgcaagcga tgctccaact
     6361 tcccgcaatt caggatcacc ttctcgtcgt ggatggcatc gaccgcggtc agcggtatcg
     6421 agaaatcgcc ggcattttcg acatagacca cgagttctgg ccggccatgc ggcgaaacct
     6481 gccgcacggc cccgaacgcc ttgtcgccat cgtggacgaa tacgtcgaag ccttcttgta
     6541 tctgttcgtt tgtcatttgc gctccgtctg ttgcacgaga ccctatcacg cccagacagg
     6601 cttcaggggc gaaccgccgc caaattccga gctggggccg ccggccgcac cgattcgagc
     6661 agcgccggaa gaacgaaaaa cgtcgtgaaa agcgtccagc caagcgagat catcagcagt
     6721 tcgcccatgc ttgcggtgcc gggatggctg gacagccaca gcgtcccgaa tccggtggag
     6781 gtcgccagcg cgctgaagaa caccgcccgc gccaggctcg attgcaggaa atcatgcccg
     6841 ccgctgcgcc acgacacgat gaaatagatc gtaaacgcga cacccacacc gaacagcagc
     6901 ggcagcacga tgatgttggc gaaattcaac ggcaatccga tgacgacaca ggtggcgaac
     6961 gtcaataatc cgatgaagat cagcgcgatc agcgccaccg ccacatcgag cgccttgcgg
     7021 aaggcaatcg tgagaagcac cgccatggcc aggaaggaca aagcccccgc ctcgatgaag
     7081 gcgcgcacga tggtgcgccc cgattcgcgc gtggagatgg ccgcaccgct tgccgccggc
     7141 gccatcgcgc tcaccgcgtc ggcaaagcgg ttcatcgcgg cttcgtcacc gacgccggcc
     7201 ttgggaaaca cttgcgtccg cgctcttccg tccttggcga tccagtcctg acgcagctcg
     7261 gcgggcaaat tctccagcgt gaccatttgc ggcatgagcg ccgcgcgtaa ccggcccagt
     7321 tcgatgttga gcgagggaat gaaggccgcc gccgcccggg ccagcgcagc ctgacctcca
     7381 ttttcgaccg catcgagcgc attcgccatg cggcgggccg cttcgtccgc gggtgacgtc
     7441 ttcgcgccgg cgagcgcgcg aagcttctcc tttgccgcat ccatgctttg aacgatttcc
     7501 gccgtatttg gcggggccat gacctcgaag ggattgagcg ttgtatccag cagcatggcc
     7561 gcatccgcga taagcgcgat cttctccgtt tgcccgtcgg gaacgaagct cttcagcgtg
     7621 cgcgtctcgc gcacttgcgg caacgcggca agtttgcgcg cggccgcctc ggctgcatcg
     7681 agcgagggcg tcagaacgtc aatcgtgtcc ggagcggttt cggggtcttt catcaaatcg
     7741 gccagcgtgg ccatcgattc cacgcgcgga ctgcgcaagt ggaacggatt ggcgtcgaac
     7801 cggatgaacg gcagcagcgc ggcgcagaga agagcggcaa cgcccgcgcc aatcacgatg
     7861 gcgcgccgcc gccggacgag gaacacatcc aggggtgcca gggcacgata accgatctcg
     7921 gcggcctctc ctttgggatg cagcaatttg agaaacgcag gaagcaacga gacgctgaaa
     7981 aaaaagatga cgatcatacc cgccccggcg ataagcccga gctgcgccag tccccggtaa
     8041 tccgtcggga cgaacgaaaa gaaacacgtc gcggtggcaa tcgcggccag ggtcagtgaa
     8101 cttcccacac cacgcacgcc ttgcaccaga gccagatcca gatcggcgat ccaatggcgt
     8161 tcggcacggt atcgcaccgc atattggata ccgaaatcca caccgatgcc gatgataagc
     8221 gggatgaagg caatggaaat aacgttgaag cttcccaccg ccgcaagccc cacggccaag
     8281 ctcatcacca ccccggccgc gagcgtcgcg agaatggcga gaatgatttt cgcggaacgt
     8341 accgcaaacc acagcatgag caggatgcaa cccgacatca gcaccaccat cagcccggcg
     8401 ttctcggtca gcgtggcgaa ttcctcatcc gaaagcggca cgggcccggt aaggcgtaca
     8461 cgcacgccct tatccggagt gagcccgcgt tccacggcga tcgcgcgcaa ctgggcagcg
     8521 gcggccgcac cgggttcgag cgcggcgtaa tccagtgcgg tgcgaacttc gatcaggcgc
     8581 cgggtctcac ggggggccgg ttttttgccg gtgatcatcg tctgccagga aagataggcg
     8641 gtgtgaccgg ccgcaacctg ttccagcgtc tcgctgaacg cggacagggc gggcgttagc
     8701 gcagccaaac tcgcctggcc gttttcgacg ccaagcaggg ccgtgttcag accgttcatg
     8761 atcccgcgca aggacggatc ggcggcgaga ggcccaagaa agggctgcgc ctggataagc
     8821 tgctgcgtgg tcgcccgcac cgcgtccgcc ggcaagtaaa gaagcccgtt atgagcaaaa
     8881 aaatcgctgc cttgcaggtc gcgcacgacc ggaaaaacag ccgggctcgc catcatctgc
     8941 gcatcgagaa tgcgcgcggc ctcctccgcg cgctccggcg tcacgccatc gatcacaacg
     9001 agcgtcagat tgtcacgctg cgggaacgcg gcatcgaacg cgagcgaatt ccggcgccag
     9061 ggaacatcct tcgcaatgag attgccggca tcgctgtcca tcgcgaagtt ccgcgatacg
     9121 taatatcctg cgccaacgcc gagaaggagg cccaggccaa ccacggcaat gcaataatca
     9181 cagcagaacc cgaccaggcg ctccagaaag cgcgtccaca tcgctagaaa tccggcaggt
     9241 tttctggtgc ttgcgtattg ttccggatct ccgcgtcacg gctttgccga taaagattcc
     9301 gcaccgtcgc gtagtaatcg atggaggtac gctggatttg ttccaccgtg tcgatattgc
     9361 gctcgcgcaa gtcgagcacc tcgaagaatc ctcgcccggc catccactcg gtcttgctcc
     9421 gcaggccgac ataggtgaac ggatccatga atatatcgac gatccttccc agaagatcgc
     9481 gcgccggcgc cggtcccaga accggcagca cgagataata accctcaccg gccccgtaga
     9541 cgccgagcgt ctggccgaaa tcctccgtgt ggctgggaat gtcgaatcgg gtcgcgacat
     9601 cgacaagccc cccgatcccg agagtcgagt tcacggtgaa acgggcgaag gtctgcgctc
     9661 cgcgggtgac atttgcctgc aacagatcat tgccgaaagt cactggcaaa tcgagattga
     9721 acaggaaatt gtggatgccg gtgcgtgcga aatcgggaac ggcgcgatga taaaatttcg
     9781 cgacgggcaa agcgatgtta tcgtcgagcc gctggttgaa atcgaaaatg gcgcgattgg
     9841 tcgattcata gggatcgttc tcgtccatcg cggcgggcat accggcgcaa ccggcgagaa
     9901 atattattga gagggtacaa aggcttgtca gaaagtgacg catgatcatc ttcataatat
     9961 cttggccgct cgattgcgaa tgtcatattg gcgtaaatcc acggcgtgaa aagtccagcc
    10021 ttcatttttc gctgtggcgg ctctaagagg cgtctcgcga atcgcgtcag ctttgagaac
    10081 cgtatgagcc acgataccct gattcatcat cttgttcgcc cctgcgtgcg atggatagcg
    10141 ccgctgcggg tgacgccaaa tcaactgacc acgttgcgcc tgctcaccgg attcagcgcc
    10201 gctgttcttt tcgcacgcgg acatggcgcg ggatttaacg ccgccggcgt ggtttttctg
    10261 atttccatgt tgttcgaccg tgccgacggg gaacttgccc ggcaaaccgg ccagatgagt
    10321 gttgccggtt atcattacga tatctggagc gattgcgcgg ccaacatcgc cgtcttcatc
    10381 ggcatcgggt tcggcctgag cggaccggag accgcactcg gtcctgccaa tatctggctc
    10441 ggcggcgtgg cgggtttggc catagccgtc ctgttctggg agctctatgt cgcgaagctc
    10501 gcttcggtgc aaggctatgc cttcctgaat gggcgcctcg ttgtcgatcc ggatgacgcg
    10561 atggtcctca ttcccattct cgtttggttc ggccttgccc aggccctgct gatgaccgcc
    10621 gcgatcatcg cttccgctgt ggctctgtcg ttcgcattcg tcgcacttcg ttccaggcac
    10681 aattcaacca aagctcagta gtaacgcaag cggacgttga tcgatccggt cattccgtcg
    10741 aaacgaaacg ctgtctcggc aaagctgggc gcgcgaaaca ggaaagacgg gtcgtttgag
    10801 aacccgaccc cctcttccgg aatcccaatc aggctccggt ccagcgtgtc attgtcgttt
    10861 tcatcgaaat aaacctgggc ggcataggtc cctgccggca cgtctccaat catcgccacg
    10921 gtctcgccct gccgcgcggg cacaatgatt ttgtaggcgc agccgtctcc gaggtagagc
    10981 gcttgcggac agatcgcgac atggagatgg ccacggccca cgcgaacatt ggaaacggcg
    11041 attcgaagca ttccagcctg tgtcggctcc gcgccgctgg cgggcattgt ggcgcacaga
    11101 gcggaaagaa gaatcacggc cgtgggaatc catgtcttca tagggcgcct tgtcgaggtg
    11161 tagaccgcat gatgatcatg gcctatgtgc ttgttgccgt gtgaaaagcc ggtttcagga
    11221 ccaccgacca taatgaaggt ttggacgctc gtcagttcgg ccatcggcgc cggcttcacg
    11281 ctgtggcttt tgtcggccta tggtttcgag cggatattgt cgcttgtcgc gatggccggt
    11341 tgggcgatcc ccgcggtggc cgcgtttcat gtgacgcaaa tcgtcgcttc cggttgcggc
    11401 tggcgcatgg ccacacgcga agacgcgcgc agattgcgcg ggcgcagcta ctatcttttc
    11461 cgttggatcc gcgaagccat caacaatctt cttcctgccg cacaattcgg cggcgtggtc
    11521 gtgggcacca ggctcatcgc ccggcgcggc gtgccattgc cttcggccat cgccggcgcc
    11581 atcgtcgata ccacgcttga attcgtcagc cagatcgctt tcacgctgct cgggctcggc
    11641 ctgctgtttc tcggcccggc cgatcgcagc gcgacgcact ttgcccttgt cgcgttcctc
    11701 gtcattggag cgctcggagt ggggcttctg gccgcgcaat ggttcggtct cgccaagctc
    11761 atcgagctgg cggccgaaaa actcggcaag gcctggggcc ggccggggcc gaacgccacg
    11821 aaaggacttc accacgagat catggcgctc tatcgcgcgc cccggcgcat cctgggcgga
    11881 ctctttttcc actttgccgc ctgggtgctg ggcgggttcg aaatctatat ttcgctgagc
    11941 gctttggggc acggttccag cattctggct tgcatggtca tcgaagcgtt cgggcaggct
    12001 ttgaaatcgg ccggcttcgc cgtgccggct tcgctgggcg tccaggaggg cggatatgtc
    12061 ctggcgggcg ccgtgttcgg tctgccgccg gaaacgggca tcgcgctttc gctgatcaaa
    12121 cgcttgcgcg acatcgttct cggtctgccg gcattggcgg tgtggcatcg tctcgaagcc
    12181 gagcctcatg tcgcggatgc tccctcccct tgaggcatga ggtttcaggc gtgggcgcgc
    12241 aatgcgtcga ggctcgccgc aaccgcgtcg atcaaccggc caaaatcgcc cggctccaac
    12301 gcgccgatgc agccgatccg gaagctttcg gctttggtga gcttgcccgg atagatcagg
    12361 aaaccgcgcg cggcaagggc ctcgtagaaa cgctcgaacg gaaattgcgg cggggagcgg
    12421 aaggtggcga tgatcggcgc ctgaatcgcc tcgtccagaa aaagcgcaaa gcccagacgg
    12481 cgcatgcctt cgagcaggac agagaaattc tgccggtagc gcgcgagccg cgcgggctga
    12541 ccgccttccg cctcgaagcg ccgcaaggct tcgacgagtg ccgccaccac ctggaccggc
    12601 ggcgtgaagc gccattggcc attggcttca aacccgcgcc attgatcgtg aagatcgaga
    12661 ctgagcgagg gacttacgcc cttggccgcc gtcagtgcgc cgctttcgat cagcgaaaag
    12721 ccaagccccg gcacgccttc aagacattta ttgctcgagg caagaaccgc cgtcaccgcg
    12781 atggtcttga gatcgatctc aagcgcaccg aaactgctca tggcatcgag caggaacgcc
    12841 cttttatgcc gcgcgacgag gtttgcgatt tccggcagtg gattgagcag cccgctcgtc
    12901 gtttcgcaat gcacgagtgc gacatgggtg atcgcgggat cgtcgccaag cgcggcatcg
    12961 accgtagcgg aatcgaccgc gcgatcctct gcccagcgca aggcacgcgc gggccgtccc
    13021 agacgattgg cgattgccat catgcgctcg ccataggcgc cattgacgag caccagcaat
    13081 ttgtccgtgg gcttcatcag cgtcgcgatg gcggcctcga caatgaaggt gccgctgccc
    13141 tggagcggca cggcgacatg ggttccggca ccgttggcca catcgagcaa tcggcgacgc
    13201 agttccgccg tcatggcgat gaaggcggaa tcgcgcgagc cccaatcgcg cagcatggcg
    13261 cggcgcgttt cgatccgggt ggtgaggggg cccggggtca acaaaatggg accggccgaa
    13321 tcgctgctca cgcggcgcgc tccgcggatt tcacccgcgc ggcatctggg ccctgcgcca
    13381 ggaaggcctt gaaccgccgc gccacctcgg cgggtgtcac ggtcgggcgt cccagcttcg
    13441 ccatggagcc gggcgcgatg cgcatatgga tgagagccgg ctttggacct tccaacgcgc
    13501 tccggaatgc ggattcgaaa ccctcaagcg tgttgcacga agcagcccac gtataaccgc
    13561 aggcgagcgc cacagcggcg aaatcgacgg acgccgacac tgtcgcctgg cctccggtcg
    13621 aatcgtgcac accattgtcg agcaggatgt ggatcaaatt gcgcgggcca taggcgccga
    13681 ccgtggcaaa ggtgcccagc ttcatcagcg cggcaccatc gccgtccaac acgacgatcg
    13741 gccgtccggt gttgagcgcg acgccgagac ccatgccggt cgcgcacccc atggagccca
    13801 cctgatagag atgctgcggc cggtcggcga tggtgaacag ctcgcgcccg catttgccgg
    13861 tggtcgcgat gatgccggtc tcgtcatcca cgacgcgaag caatcgttcc agcacggcgg
    13921 cgcgggccgg ctgcgttcct gcctgctcgt tgcggatgcg ctggccggcg acgcgcgaag
    13981 ggagcggcgc ggaatcgagc ggttcctcct ccacatccac tttgcgcatt accagcgcaa
    14041 acggctgcgc gcttgcgtcc atgtgctcca cggcgcggtc cagcgccggc ccgattgccg
    14101 caacgtccgc cgggaatggc gcttgcgcga ggccgatgag cgacaggaga tcctgcgtga
    14161 tccggcccat cacctcatgt tgcggttcat cggcgatggc gggctcgccc cgccaagtgg
    14221 tgatgaaaag cgtgggaatc ttgaacggcg cgttgagcga tgtcagcgga ttgaccgcgt
    14281 tgccgagtcc cgagttctga cacatcacga cagtgcgccg tccggccagc caggcgcccg
    14341 atgcgatcgc cacggcttcg ccctcactcg ccgcacccac ataggagaga ctacggtcgc
    14401 tcagcacgcc gttgatgagc ggcgtcaaaa agctgcacgg cacgccggtg aagaaatcga
    14461 agcccctgcc tgtggcttcc tgcaaaaagg cgcgggcgct gatcatcgag gcacgatttt
    14521 tttccttatc gggtgaattg cgggtcaggt gaaattgcgg gtcaggtgaa attgcgggcg
    14581 ttcgccaggt cctggaccgt gtcgacatcg agccaatgtc cggtgaaata atgaacgctc
    14641 accggatgtt tcgcagtcaa ccggttgaag agaagcggaa gatcgccgcc ttccacgagg
    14701 ccttcggcgg tcatcgcggc gatctcctcg cgcatccact cggcaccctg cccgctcaaa
    14761 cgcgcaagac ccatccactc cccactgatt ttgtccttcg gcaaatccgg cgccatggcg
    14821 cgaagataca ccggctcctc gtcaagatag aggccggaaa acggacgatc cgccgcgacg
    14881 agatcgcgat attgctggcc ggactgctcg aacagcttca gcgcatcgac agcgagcacg
    14941 atatcgccct tcacggccat gagctcatcg aggatatatt ggcggaacaa cacatcgcca
    15001 tagacgagga tggtttcgcc atcgagccgg tcaccggcgc gtgccagcga ataaagctcg
    15061 ccggtttcgg catggcgctc gttatcgatg atcgagatgc cctgaaccgt caccgcgtcc
    15121 ttgcgataac cgcacaccac gctgacattg cggatgccgc tgtcggcaag ggtccagaca
    15181 agccgctgca gaagcgcatg cccgcgcaca tcgatcatgc atttcggccg gtcctgggtc
    15241 agctcgccga gccctccgcg cgaagccgca agcacgatgg cgcgggcggt cgccctgtcg
    15301 ggcagataac gctgttcggc agcttccaac tcggcatttc cggccagcgc gaagacgtcc
    15361 ttcacgctcg cgacacggcc ttcgatctcg gtcagcgatt gatcttcgaa gatgcgccgc
    15421 gcggtctcgc gcatcgcggt gatcgacgcc ctgagtgcgt gattcgccca gatgacggcg
    15481 gagatgcggg cctggcggaa atgttcggtc ggcgtcgcgt aataggttgt gggcacgatg
    15541 acgagcggcg cgcgattggc ccaacgctcg gcaaaggcaa ggatttcgga ggctgtcgta
    15601 agcttggaat ggatcaggat cgcatcggcg ccggccttcc aataggcctc cgcgcgggtc
    15661 agagcctcgt ccatgccgcg ccccgagatc agtgcctcca cccgcgcgac caatacaaaa
    15721 tcatcgtcgg tctggctgtc ctttccggcc tttatgcggc cgcagaactc gtcgatgtcc
    15781 gcgagcggct gggccgagcc gatgaaggaa ttcgtcttgg ggaacagctt gtcctcaata
    15841 caaaccccgg caatgccgtg tgcgcagagc ttgccgacga gacgccgcac attgttgaaa
    15901 ttgccgtaac ccgaatcgcc atcgaccaag atagggagtt gcgtcgcatc gctcatgaat
    15961 tcgagcacat cgagaacctg cgtccacgag gcttcgttgc tgtcgcgcag gcccaggctc
    16021 gccgaaatgg taagcccgga cgcccacagc gcgctgaacc ctgcctcttg ggcaatcttg
    16081 gccgaaagcc cattatgggc ctccatcagg aatgacagct cgggagtttc gatctgccgg
    16141 cgaaggtcgg cagctttcga ggggcgttcg gttgccggcc tcaaggcgat ggtgtggcga
    16201 tcttggatgt tcatggcgct cttgctattg cgtttcgttg ttgccgggac cggtttgctc
    16261 tagcgcaatg tccctgtaat ctcggcaggc tatcggatta cctattggag cgtcaaaatt
    16321 ttgtcatctg ttccaatgaa atccgcttcc cctcccctga ccgccattat tctcgccgcg
    16381 ggcgtgggaa ggcggttggg cgcggccgac ggcaaaccga aggttctgct cgaattcggg
    16441 ggaaaaacgc tgctgcaacg ccatctcgaa gcgcttcacg cgcatggcgt gacggacatc
    16501 gccctgaccg tcggccacca agccggtgcg attcgcgccg ccctggaagc cctcggctat
    16561 ggggcccgtg tggcctgcgt cgagaataca cgataccgcg agggcagcct ggtatcgctg
    16621 tgggcgcagc gcgcacgcct tcaagcaggc tccgaggtcg ttctgatgga tggggatgtc
    16681 ctctatgatt cgcgcatgat cggccggctt ctcggtgccg cgccggaaaa cgtgctgctg
    16741 gtcgaccgcg agatcgagcc gggcgacgaa ccggtcaaga tctgctttcg cgacgggcgc
    16801 atcgtggatt tccgcaagaa gcccgaccag cctcacgatt ggcatggcga atcggtgggc
    16861 ttttttcgct tttccccttc catggcacaa gcgttggccg ggcaatgcgg gatctatgtc
    16921 gaagagggcc gcgcaaatgt cgaatatgag gaggcggtac gcgacctgct attgcgggaa
    16981 ccttcgcgtt tcggcgtcga ggacatcagc gatcttccct ggacggagat cgatttcgac
    17041 gcggatgttg cgcgggcccg cgacgaaatc cttccgcaat tgtgaagacc ccgatgacac
    17101 agacggccct ggaaaatccg ttgagcgatg ccgatctttc gcaatatgaa cgcaccggct
    17161 gggttgtgcg gcgtggtttt ttcgatgccg aggacgccgc cgccattgcg cgatggaccg
    17221 acgaggtgac ggcgatgccc gagctgccgg gcagacagat ggtctattac gagccgagcc
    17281 tcgttgcgcc ggacgcgcgg gtcattcaac gcatcgagaa tttctgcccc tatcacgaaa
    17341 gcttcgatgc gctggtccgt cgcagccggc tcacgcgcgc cgccaatgcc ctcctcggcg
    17401 ctccatcgtg cctgttcaag gagaagatca actacaaaat ggcgggcggc gccggcttcg
    17461 agccgcatca ggatcagcag gccggctggt cgcgctatgc gcccatcttc gtgacggcgc
    17521 tcgtctgcat cgatcgcgcc acagtcgcga atggctgcct ggaaatgccc accgtgcggc
    17581 ggctgaaaca gatgatcgga gcggaatggc gcccgctcac ccgcgatgag atggcgtcgt
    17641 ttgaacttgt tccggtggag accgaacccg gcgacgtcat tttcttcgac agctatgtcc
    17701 cgcacagctc gcagccaaat ctcacgagcg acgcgcgccg cattctgtat ctgtcctaca
    17761 atcgcctgtc cgacggcgac cacaggctgc aatatttcgc ggaaaagcgc gccaatttcc
    17821 cgcccgatat cgaacgcgcg ccgggcaagg actataaatt ccgggtctaa gcggccttat
    17881 tgaccggcca tttgcttctc gacgagatcg tcgatcttct tcaccagccc cgcggcgccc
    17941 gaactcgcga tcgtcgcggc gaaatcggag cggcgcaccg caagctggct gacataaccg
    18001 ttcagataga tgtccacgat cttccatttg ccgtcggggg cgagatgcat ccgataattg
    18061 aacggcacgg ttgaactgcc gctcatcaat ttcgattcca cgattttctc gtcgttgcgc
    18121 atcttcacca tgggatcgac ggtgaatttc tgccccgaga aaccggcgaa attcgccgcg
    18181 taattcgcaa tcgtcatgcg ttcgaaagca tccgtgatcc gcttgcgatc ggcttcggag
    18241 agcatttgcc aagccgggcc caccgagagc tgtgccatag ccggcagatc gaatgcctcc
    18301 cgcacggcgg gagacatttg gcgatagcgg ccctgcagtc ccagttgctg gccctgcttc
    18361 atggtgtcga tcaatacgga ataaaacatt tcgatctgac gcgcggcggg atcgggcgcc
    18421 ggatcggcgg cacgcgcggg cgatatcccc ggcgacagca agagcaggaa cgccagcgcg
    18481 gtccacaatc tgagaaatgc catcggacgg ccgttgtctg cgaatttcat caggagtctc
    18541 gctctacgag aaataacgca agctctcgca aatcggttgc cgcatcgcca tagcattcaa
    18601 gcacgccggc cgcgtgctgc gccaaaatca tggctttgtt gcgcgccccc tcgatcccca
    18661 ggagggagac aagggttgcc ttgcctctat ctttatcctt gccgacggat tttcctgtct
    18721 tctcggccga accgagcaca tcgagcagat cgtcggtgat ctggaacacc aggccgagat
    18781 cgcgcgcata gacgtgcagc ctgtcatgat cttcgggagc ggcttgcgcg aggatagggc
    18841 ctgcggcgca ggaaaattcc agcagagccc ccgtcttcat ttgctggagc gtgacggtct
    18901 cttcggcatt gaaggggcgg ctttcggctt cgatgtcgat gatctggccg ccgatcatgc
    18961 catcatggcc ggccgccctc gcgagccgtt ccacaagctg actgcggact tccggcgagg
    19021 gatgggtctc gcgattcgag aggattccga acgcaacggt gagcagtgca tcgcccgcga
    19081 gcacggcggt cgcctcatcg aacgccacat gagtcgtggg acggccgcgc ctcagattgt
    19141 catcgtccat gcagggcaga tcgtcatgga ccagggaata ggtgtgcagc acttcgaccg
    19201 cggccgcagc ccgcaaggca caggccgcat ccaccccgaa caaagccgcg gcacgcagga
    19261 ccaggaaagg ccgcagccgc ttcccgcccg caaaa
//
LOCUS       DASYEG010000009         8584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_5076574, whole genome shotgun sequence.
ACCESSION   DASYEG010000009 DASYEG010000000
VERSION     DASYEG010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 8584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8584
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5076574"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="VGM72_00540"
     CDS             complement(<1..1077)
                     /locus_tag="VGM72_00540"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005085.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEY4263777.1"
                     /translation="MRTTPPFRADHVGSLLRTAAIKDARARRGKGAIGAAEFKDIEDR
                     EIGGIIRRQKAIGLQSATDGEFRRAWWHFDFYGQLEGVELRDVSSGIQFQGVQTKAQA
                     PHIVGPLGFPANHPMLEHFRFLKAHTDAAAKMTIPSPSVLYFRAGSVNAKLYPDRAAL
                     FASLADVYIQAVKAFYAAGCRYLQFDDTAWAYLCSQDEVEKAKARGTDVENLGSAYAG
                     MINRVLAEKPEDMAITTHICRGNFRSTWITSGGYEPVAELLFGACNYDGYFLEYDSDR
                     AGGFEPLRFVPQGAKQIVLGLVTTKTGLLEKKDDIKRRIGEAAEYVALDQLCLSPQCG
                     FASTEEGNILAEDEQWTKLSLIVDI"
     gene            complement(1094..2122)
                     /locus_tag="VGM72_00545"
     CDS             complement(1094..2122)
                     /locus_tag="VGM72_00545"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4263778.1"
                     /translation="MRIIDSHFHWWPRSVFEQLCKRTGFPHCERNARGGYNYVGHEGR
                     PSLNSWTEWFDLDRQLEHMDGLGHQVGVVCSIGPFSISFSDLPPEEGRDLAFAWNEEM
                     AGAQRKYPGRVWASAAVPLTDTKIAIDVLDHAILKLGLMGVNIPGSIGRDTRIDAERL
                     APFYARIEELGVPLFLHPTDAVFGDDLEGYGGALQLSLGRVIEVSVTAARMVFSGLME
                     RHPNLRIVMSHTGGALPYQSGRMDKNGKAAKLPKPPSTYIRRMYTDTVSPHSMGMKFA
                     IDYYGIDQVMYGSDYPCWDPATALTLIEEIGLPEADREKLFYSNARRILGLRDPERQT
                     ASASVAAE"
     gene            complement(2128..3447)
                     /locus_tag="VGM72_00550"
     CDS             complement(2128..3447)
                     /locus_tag="VGM72_00550"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY4263779.1"
                     /translation="MILRSWAENLLQTAGTPDAIMLHLMDTLAAFLAGAATGDGQALS
                     TLYGRRGDAGEIAAGAAAIARASECDDIHLASCVTPGAVVIPVALTLAENRSEDDFAR
                     AVAAGYAAGLSLGIGIGGAKALGAGVWPTLLAAPLMAAVTASCLAGHDAERLGHAMAL
                     ALAGASGRLGRPAGTPSGRWFSLAEAVLKGIRASAAAGDGFRGDLSLISEPWLVPHAG
                     HANVDMAVFESPASAPSIGDVGYKPFPIARQGANAIAALQRALSNGVDPRRIDGIEIF
                     VPAIHTALLNRPVQANDRLSRLSNMAYQCACAALAPDMLYDVERRQQPDIPLMDFARR
                     VSVVPSKDMDAHLPGRWAARVAIATGNGRVEETVIAAPFDHDTPRIGKLLDEKWRRLL
                     PAQDAADFFTLATAGASAEERAILWKMIEGHVSMTASPGRKPEPGEG"
     gene            complement(3444..4505)
                     /locus_tag="VGM72_00555"
     CDS             complement(3444..4505)
                     /locus_tag="VGM72_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEY4263780.1"
                     /translation="MRRGLMAWNADEIPLAMLETRVLRLQDAMAAAGQDAMILYTNFI
                     RSAAVSYLTAFSPYWADGILLVPQEGAPLFATTLSKRVGSWIQSVKPVGDLVTSPTPG
                     TVLGKKLAEAGNIHRLAILEHDAFPAGLHDELSAALPGVEIVDGTAVFALARQHVDDV
                     ERRLLTRADAIADSALHRLQIDATMDVGRAVGTVEGVARAQGAEEAYVAIAPDLDADR
                     RFLRLSGARPLGRRFAIRATVAYKGAWIRRIQTYASDDSDGFAIARADSWFKDFAAKI
                     DRNRTVGEQISASLTGLSGARLTGWMAEGPLGTRPLAMVASSKTPNEMTLRTPVLVMT
                     IALNVDGVPWSGAGLAGLP"
     gene            complement(4515..5627)
                     /locus_tag="VGM72_00560"
     CDS             complement(4515..5627)
                     /locus_tag="VGM72_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY4263781.1"
                     /translation="MSGAPIAEARRARLSEAMAEEGVDLLLVYGNAWQSDYLRYATDY
                     GILEGEALAIIRRDGTTTLFLDSPLEADRAGVESPGVETVYAPAMLAEVEKALERAGN
                     SRIATAPKRLMPHRIAARAKDFTLGDGTLMMDRLLMKKLDCEIAAIRAAAHMADEGYG
                     VFRKAAREGRADYELVAEVEAFFRARGVDDNFMIIGVGGKEVRGMAPPMGKRLKPGDL
                     VTTELTPCVNGYYIQICRTLVMGEPNAAQCAAFGVYRDALKAGVAAVKPGVTAADIAH
                     AENEIFRAHGLGEYVTEAYTRVRGHGLGLFADTKPHILENVTTKIEENMTMIVHPNTY
                     HPEVGYMVLGDAVIVISGGADVLTKTPRELFSVPAH"
     gene            complement(5624..7300)
                     /locus_tag="VGM72_00565"
     CDS             complement(5624..7300)
                     /locus_tag="VGM72_00565"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY4263782.1"
                     /translation="MTQAHPITVEVVRNAVVAYADEMANVLAKSAYNMMIYEVRDYCC
                     GLLDTKGQMISQNRGGLPIFLADLGPSVEDGIRKYGLDGFKPGDIVIMNHAGVCGQHL
                     NNVVIYAPCFHAGELVGFAANRAHWVDIGGMRQGFGSSATTEIFGEGLQLRSLKIYEA
                     GARNETLWQILRDNIRFPDASLGDLRAQIAACQVGAERYAELIARYGRETVEDCIAVV
                     WDQAEAAARRVIETIPDGTYEAESFLDNDGRNLDVRLRVKVKIVVAGSTMTVDYSEMN
                     KQVASPLNSGRSGGIAAARVAFKALTSPDLDVNEGCFRPLDVILPEGTMLSAKPPAAL
                     GLWSIALPTVIDTILKALAPAIPDKVPAAHKGDMGGCSFFGFREDNGSRFLLLNIFGG
                     GWGGRPGEDGESASVSICQGDVRNTPIELQEIKYPFLIEQHALRADSGGAGRTRGGLG
                     VAITYRCLQACKANINLERLTDPPWGLHGGRPGAINLAIIARANGQESEVRKQTEIEL
                     QAGDRVTFLTAGGGGYGAPSERARETIARDLAEGFISAAGAEAYMAMGTS"
     gene            complement(7297..>8584)
                     /locus_tag="VGM72_00570"
     CDS             complement(7297..>8584)
                     /locus_tag="VGM72_00570"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY4263783.1"
                     /translation="GMIGAGRLARDLKLETCIGFDMGGTTAKTSLITHGAPAIDEGYV
                     IGGAASGQPMQLPVVDIVEVGAGGGSIAWIDANGGIHVGPQSAGADPGPACYGKGNED
                     PVVTDANLVLGRINPRRFLNGHMALDVAASERAIHDKIAAPLNLSVRDAALGIIRIAD
                     AAMSLAVRAVSVNKGVDPRDTTLIAFGGGGPLHAVAIAREIFIPKVVVPKLPGTFSAL
                     GMLMASWRQDFVRTLIGRLGTLSPPEVERVFGELTAAGEERIARDSIAKAAADFTFYA
                     NLRYVGQEHTIPIPVAGPRMLTGELAALRTLFHAEHAKRYSQSAPDESMEIVSLRLVV
                     TSARADNLAERWLNEPWQTQAPMEELRREVVFGDPERPLATRILWRPSLPAGTIVEGP
                     VIIEEPNATTLVHPGDTASVTAAGHLMIAVGQEIFS"
BASE COUNT         1618 a         2716 c         2654 g         1596 t
ORIGIN      
        1 gatatcgacg atcaggctga gcttggtcca ttgctcatct tcggcgagga tgttgccttc
       61 ctcagtgctg gcaaatccgc attgcgggct gaggcaaagc tgatcgagcg cgacatattc
      121 tgccgcttca ccgatgcggc gtttgatgtc gtctttcttc tccagtagtc cggtcttggt
      181 cgtgaccagg ccgagcacga tctgcttggc accttgcggc acgaagcgaa gcggctcgaa
      241 accgccggcg cggtcgctgt catattcgag gaaataaccg tcgtaattgc aggcgccgaa
      301 cagcaactcg gccaccggct catagccgcc cgaggtgatc caggtcgaac gaaaattgcc
      361 gcggcagata tgggtggtga tcgccatgtc ctcgggtttt tcggccagca cgcggttgat
      421 catgccggca taggcgctcc cgaggttttc cacatcggtg ccgcgcgctt tggctttctc
      481 aacttcgtcc tgggagcaga gataggccca agccgtgtcg tcgaattgca gatatcggca
      541 acccgccgca tagaacgctt tcaccgcctg aatgtagaca tccgcaagcg acgcaaacag
      601 cgcggcacga tccggataaa gtttcgcatt caccgagcca gcgcgaaaat acagcacact
      661 gggactggga atggtcatct tcgctgccgc atccgtgtgc gccttcagga agcggaaatg
      721 ttcgagcatc gggtggttgg ccggaaagcc cagtggcccg acgatatggg gcgcctgcgc
      781 cttggtctgc accccctgaa actggatgcc tgaggaaacg tcccgcaatt ccacgccttc
      841 gagctggccg tagaaatcga aatgccacca ggcgcggcga aattcgccgt ccgtcgcgga
      901 ttgcaggccg atggctttct gccggcggat gattccaccg atttcgcggt cttcgatgtc
      961 tttgaattcg gccgcgccga tggcgccttt cccacggcgt gcccgcgcgt cctttatggc
     1021 tgcggtgcgc aacaggctgc cgacatggtc ggcacggaat ggcggggttg tcctcatcgg
     1081 ggggagcggg gtgctattcc gccgcgacgc tggcggacgc ggtttgccgc tccggatcgc
     1141 gcaggcccag aatgcggcgc gcgttgctgt agaacagctt ctctcggtcg gcctccggca
     1201 agccaatctc ttcgatcagc gtcagcgccg ttgccggatc ccagcaggga taatccgagc
     1261 catacatcac ttggtcgatg ccgtaataat cgatggcgaa cttcatcccc atgctgtgcg
     1321 gcgacacggt gtcggtatac atgcggcgga tataggtgct gggcggctta ggcaatttcg
     1381 cggccttgcc gttcttatcc atgcggcccg attgatacgg caatgcgccg ccggtgtgcg
     1441 acatcacgat tctaagattg gggtggcgct ccatcaaacc ggaaaagacc atgcgcgcgg
     1501 cggtgacgct gacttcgatc acgcgtccga gactgagctg aagcgcaccg ccatagcctt
     1561 ccagatcgtc gccgaacacc gcgtctgtgg gatgcaggaa aagcggaaca ccgagctctt
     1621 cgatccgcgc atagaacggc gccaggcgtt ccgcgtcgat gcgcgtatcg cggccgatgc
     1681 tgcccggaat gttgacgccc atcaatccga gcttgaggat ggcgtggtcc agaacgtcga
     1741 tggcgatctt cgtatcggtc agcggcaccg cagcacttgc ccagacccgg cccggatatt
     1801 tgcgctgggc gcccgccatc tcctcgttcc acgcaaaggc caagtcgcga ccctcttccg
     1861 gcggaagatc ggaaaacgag atcgaaaatg gtccgatgga gcaaacgacc ccaacctgat
     1921 ggccaagccc atccatatgt tcgagctgcc ggtcgaggtc gaaccattcg gtccaggaat
     1981 tgagcgacgg ccgcccttca tgtccgacat aattataacc gccccgggca ttgcgctcgc
     2041 aatgcggaaa gcctgtccgc ttgcaaagct gctcgaagac gcttctcggc caccaatgga
     2101 agtgggaatc gatgatccgc atgatgctta tccttcgccc ggttcgggtt tgcggcccgg
     2161 gctggccgtc atgctgacat gcccttcgat cattttccag agaatggcgc gctcttccgc
     2221 gcttgcccct gctgtcgcaa gcgtgaagaa atccgctgca tcttgggccg ggagcagccg
     2281 ccgccatttt tcatcgagca gctttccaat gcgaggggtg tcatggtcga atggtgcggc
     2341 gataacggtt tcctcaaccc gtccattgcc tgtggcgatg gccacgcgcg ccgcccagcg
     2401 gccgggaaga tgcgcgtcca tgtccttcga agggaccacc gacacgcggc gcgcgaaatc
     2461 catgagtgga atatcgggtt gttgccgccg ctccacatcg taaagcatgt ccggagcgag
     2521 tgccgcgcac gcacattgat aagccatgtt cgacaaacgg ctgaggcggt cattggcctg
     2581 aaccggccgg ttcagcagcg cggtatgaat cgccggcaca aaaatttcga ttccatcgat
     2641 ccggcgtgga tcgacaccgt tggacaatgc ccgttgcaag gcagcgatgg cattggcgcc
     2701 ctggcgcgca atcgggaagg gcttgtagcc gacgtcgcca atagatggcg ccgatgcagg
     2761 cgattcgaat accgccatat cgacatttgc atggcccgcg tgcggcacca gccatggctc
     2821 ggaaatgagc gacaggtcac cacggaagcc atcgcccgcc gcggcagagg cgcgaatgcc
     2881 cttcaacacg gcttcggcga gcgaaaacca acggcccgat ggcgtgcccg caggacgacc
     2941 gagcctcccg ctggcaccgg cgagcgccaa tgccatcgcg tggccgagcc gctcggcatc
     3001 atgcccggcg aggcatgacg ctgtgacggc ggccatcaga ggtgcggcaa gaagcgtggg
     3061 ccagacgccg gcgccaagcg cctttgcgcc gccgatccct attccaaggc tcaagcccgc
     3121 agcgtaaccc gccgcgacgg cgcgcgcgaa atcatcctcg ctgcgatttt ccgcgagagt
     3181 caacgccacc gggatgacga ccgcgccggg cgtcacgcat gaggcgagat ggatatcgtc
     3241 gcattccgat gcccgcgcga tggccgcagc acctgccgcg atttcgcccg catcgccgcg
     3301 tcttccgtat agcgtggaaa gggcttgacc gtcgccggtt gcggccccgg caagaaatgc
     3361 ggccagcgta tccatcagat gcagcatgat tgcgtccggc gtgccagccg tctgcaaaag
     3421 attttccgcc catgatcgca ggatcatgga agacccgcga ggcccgcgcc ggaccaaggc
     3481 acgccgtcga cgtttagagc tattgtcatg acaagaaccg gcgtgcgcaa cgtcatttcg
     3541 tttggcgttt tggaggatgc gaccattgcc aagggacgcg tgccgagcgg tccttccgcc
     3601 atccagccgg tgagccgcgc ccccgagagg cccgtaagcg aagccgagat ctgttcgccc
     3661 acagtccggt tcctatcgat cttcgcggca aaatccttga accaggaatc ggcgcgcgcg
     3721 atagcgaaac cgtcactatc atcgcttgca taggtttgga tgcgccggat ccaggcgccc
     3781 ttataggcaa ccgtcgcgcg gatcgcgaag cggcggccaa gaggacgggc accggataaa
     3841 cgcaggaaac ggcgatccgc atccaggtcg ggagcaatcg ccacataggc ctcttccgcg
     3901 ccttgggcgc gtgcaactcc ttcgacggtg ccgacagcgc gccctacatc catcgtcgca
     3961 tctatctgca aacggtgcag cgcgctatcg gcaattgcat cggcccgcgt cagaagcctg
     4021 cgctcgacat cgtccacatg ctgacgcgcc aaggcaaaga ccgccgtccc atccacgatc
     4081 tcgacaccgg gaagtgccgc ggacaattcg tcatggaggc ctgcgggaaa tgcatcgtgc
     4141 tccagaatcg cgagacgatg gatattgccc gcttccgcca attttttgcc gagcacggtg
     4201 cccggtgtgg gcgaggtgac cagatcaccc accggcttca ccgattgaat ccaactgcca
     4261 acgcgtttgg acaatgttgt tgcgaacagc ggcgcgccct cttgcggcac gagcagaatt
     4321 ccatccgccc aatagggcga gaacgcggta agataggaga ccgcagccga gcgtatgaaa
     4381 ttcgtataca ggatcatcgc atcctgcccc gccgccgcca tcgcgtcctg cagccggagc
     4441 acgcgcgtct ccagcatcgc aagcggaatc tcgtccgcgt tccacgccat caaccccctt
     4501 cgcatggcat tctcctaatg ggccggtaca gagaatagtt cgcgcggtgt cttggtcagg
     4561 acatcggcgc cgccggaaat cacgatcacc gcatcgccga gcaccatgta tccgacctcc
     4621 ggatgatagg tgttggggtg gacgatcatg gtcatgtttt cttcgatttt tgtggtcacg
     4681 ttttccagaa tgtgcggttt ggtatcggcg aaaagtccaa gcccgtgacc gcgcacgcgc
     4741 gtataggcct cggtgacata ttcgccgagc ccgtgggcgc ggaagatctc gttctcggcg
     4801 tgggcgatat cggccgctgt gacgccgggc ttgaccgccg cgacaccggc cttcagcgca
     4861 tcgcgataga caccgaacgc cgcgcactgt gcggcattcg gttcgcccat gaccaatgtg
     4921 cggcagatct ggatgtaata accgttgacg cacggggtca gctccgtggt gacgaggtcg
     4981 cccggcttca gacgcttgcc catgggcggc gccatgccgc gcacctcttt cccgccgaca
     5041 ccgatgatca tgaaattatc gtcgacgccg cgcgcgcgaa agaacgcttc gacttcggcg
     5101 acaagctcgt aatcggcccg gccctcgcgc gccgctttgc ggaagacgcc gtacccctca
     5161 tcggccatat gggccgccgc acggatggcg gcgatttcgc aatcgagctt tttcatcagc
     5221 agccggtcca tcatcagcgt gccgtcgccg agcgtgaagt ccttggcgcg tgctgcgatg
     5281 cgatgcggca tcagccgttt gggcgcggtg gcgatgcgcg aattgccggc acgttcgagg
     5341 gccttctcca cctcggccag catggcgggc gcgtagacgg tttccacgcc ggggctttcg
     5401 acgccggcgc ggtcggcttc gaggggcgaa tccaggaaca atgtcgttgt tccgtcgcgg
     5461 cgtatgatgg cgagggcttc gccttccagg atgccgtaat cggtcgcata gcgcagataa
     5521 tcgctctgcc aggcattgcc atagacgaga agcagatcga cgccctcttc ggccatcgct
     5581 tcggaaagac gcgcgcgccg ggcttccgcg atgggagcgc cgctcatgat gtgcccatgg
     5641 ccatataggc ttcggcgcct gctgcggata tgaaaccttc ggcgagatcg cgcgcgatcg
     5701 tctcgcgggc gcgctcagac ggggcgccat aaccgccgcc gccggcagtg agaaaggtca
     5761 cgcggtcgcc cgcctgaagc tcaatctcgg tttgtttacg gacctcgctc tcctgaccgt
     5821 tcgcgcgggc gatgatcgca agattgatcg cgccggggcg cccgccatgc aatccccaag
     5881 gcggatcggt cagccgctcc aaattgatgt tcgctttgca ggcttgcagg cagcgatagg
     5941 tgatggcaac gcccagcccg ccgcgcgtgc ggcccgcgcc accgctatcg gcgcgtagcg
     6001 cgtgctgctc gatcagaaaa gggtatttga tctcctgcag ttcgatggga gtgttgcgga
     6061 catcgccctg gcaaatggat acgctggcgg attcgccatc ctcgccggga cgcccacccc
     6121 agccgccgcc gaaaatattg agcaaaagaa atcggctgcc attgtcctca cggaagccaa
     6181 agaaggaaca cccgcccatg tcgcccttgt gcgcggcggg aaccttgtcg ggaatggctg
     6241 gcgccaatgc tttcaggatg gtgtcgatga cggtgggaag cgcgatgctc cagaggccga
     6301 gcgcggcggg cggcttcgcg ctcagcatgg tgccttcggg caagatcacg tccagcgggc
     6361 ggaagcagcc ttcgttgacg tcgaggtcgg gcgaggtcag tgccttgaaa gcgacgcgcg
     6421 ccgccgcgat gccgccggag cgccccgaat tcaatgggct cgccacctgc ttgttcatct
     6481 cgctgtagtc gaccgtcatg gtcgagcccg cgacgacgat tttcaccttc accctgaggc
     6541 gcacatcgag attgcggccg tcgttgtcga ggaagctttc tgcttcatag gtgccatcgg
     6601 gaatggtctc gatcacgcgc cgggccgcgg cctcggcctg atcccagaca acggcgatgc
     6661 agtcttccac cgtttcgcgg ccatagcgcg cgatcaactc ggcgtaacgc tcggcgccga
     6721 cctggcaggc cgcaatctgc gcgcgcaaat cgccaaggct cgcatccgga aagcggatat
     6781 tgtcgcgcag gatttgccac agcgtttcgt tgcgcgcgcc ggcctcgtag attttcagcg
     6841 aacgcaattg cagtccttcg ccgaaaatct ccgtggtcgc gctcgacccg aaaccctgcc
     6901 gcatgccgcc gatatccacc caatgggcgc ggtttgccgc gaagcccaca agttcgccgg
     6961 cgtggaaaca cggcgcatag atcacgacat tgttcagatg ctggccgcac acccccgcgt
     7021 ggttcatgat gacgatgtcg ccgggcttga agccgtcgag gccatatttc cggatgccgt
     7081 cttccaccga aggaccgaga tcggcaagga agatcggcaa gccaccacga ttttgcgaaa
     7141 tcatctggcc tttcgtgtcc aacaggccgc agcaataatc gcggacctca tagatcatca
     7201 tgttgtaggc ggatttcgcg agcacattcg ccatttcatc cgcataggct acaacggcgt
     7261 tgcgcacgac ttccacggtg atgggatggg cttgggtcac gaaaaaatct cctgaccgac
     7321 ggcaatcatc agatgtccgg ccgctgtgac ggacgccgtg tcgccgggat ggacgagggt
     7381 ggtcgcgttg ggttcctcga tgatgacggg gccttcgacg attgttcccg cgggaagcga
     7441 agggcgccac aaaattcgtg tcgccagcgg gcgttcggga tcgccgaaca caacttcgcg
     7501 gcgcaattct tccattggcg cttgcgtttg ccacggctcg ttcagccagc gctcggcgag
     7561 attgtcggcg cgcgcggacg tcaccacgag gcgcaggctc acgatctcca tcgattcatc
     7621 cggcgccgat tggctatagc gtttggcgtg ttcggcatgg aacaaggtgc gcaaggccgc
     7681 gagttcaccc gtcagcatgc gcgggcccgc gaccgggatc gggatcgtgt gctcctgccc
     7741 gacatagcgc agattggcgt agaaggtgaa atcggcggcg gccttagcga tggagtcgcg
     7801 cgcgatccgc tcctcaccgg cggccgtcag ttcgccaaac acgcgttcaa cctcgggggg
     7861 cgaaagcgtg ccgagccttc cgatcagggt acggacgaaa tcctgccgcc acgatgccat
     7921 cagcatgccc aatgccgaga acgttccggg aagcttaggc acgaccacct tggggatgaa
     7981 gatctcgcgg gcgatagcga cggcatggag tgggccgccg cctccgaatg cgatcagcgt
     8041 cgtatcgcgc ggatccacgc ccttgttgac ggaaaccgcg cggaccgcga gcgacatcgc
     8101 ggcatcggcg atgcggataa tgccgagcgc agcatcgcgc acgcttaaat tcaggggggc
     8161 cgcaatctta tcgtggatcg cgcgctccga ggccgccaca tcgagcgcca tatgaccgtt
     8221 caggaagcgt ctcgggttga tccgtcccag gacgagattg gcgtccgtca caaccgggtc
     8281 ctcattgccc ttgccgtagc aggctgggcc aggatcggcg ccggcacttt gcgggccgac
     8341 atgaatcccg ccattggcat cgatccaggc gatggacccg ccgcccgcac cgacctccac
     8401 gatgtcgacg acaggaagct gcatcggctg gccgctggcc gcaccgccga tgacatagcc
     8461 ctcatcgatg gcgggcgcgc catgagtgat gaggctcgtc ttggccgtgg tgccgcccat
     8521 gtcgaaaccg atgcaggttt ccaacttcag atcgcgcgcg agccggcccg ccccgatcat
     8581 cccc
//
LOCUS       DASYEG010000010        19274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3772531, whole genome shotgun sequence.
ACCESSION   DASYEG010000010 DASYEG010000000
VERSION     DASYEG010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 19274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19274
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3772531"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..3037)
                     /gene="secA"
                     /locus_tag="VGM72_00575"
     CDS             complement(164..3037)
                     /gene="secA"
                     /locus_tag="VGM72_00575"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY4263784.1"
                     /translation="MLSLDSFIQRPLNALTGLFGSVNDRKVKPKLATVAKINALEPAF
                     EKKSDAELLALTATFKQRFQNGETLNDLLPEAFAAVREAAKRTLGQRHFDVQLIGGMV
                     LHEGRIAEMKTGEGKTLVATLPVYLNALEGKGVHVVTVNDYLATRDAEWMGQIYNFLG
                     LSVGTIVHGLTDEERRVNYAADVTYGTNNEFGFDYLRDNMKYNLDQMAQRGHHYAIVD
                     EVDSILVDEARTPLIISGPTEDQSELYKKVDSILSGLVAEDFELDEKQRQVSLTEQGN
                     EHMVELLRSVDALEEGDLYDINNIVVVHHVNQALRAHKLFQRDKDYIVKDDKVVIIDE
                     FTGRMMEGRRYSEGLHQALEAKEHTSIQSENVTLASITFQNYFRLYGKLAGMTGTALT
                     EAAEFMDIYNLDVIEIPTNMPVKRQDADDEVYRTEDEKHRAIVTQIAECHMKGQPVLV
                     GTVSIEKSETLDAKLRDRKYIRETGEELIERAQKLGDTPQGKEHREIGEYLVSISSKS
                     NPIEHNVLNARYHEQEAYIVAQAGRSHGVTIATNMAGRGTDIQLGGHPDMRIRLELAD
                     VTDEAERARRIAEIKAEVVADKQKVIGAGGLFILGTERHESRRIDNQLRGRSGRQGDP
                     GASKFFLSLQDDLMRIFGSERMDSILQRLGLEKGEAIAHPWVNKALEKAQQKVEARNF
                     EMRKNVLKFDNVLNDQRKVIFEQRLDIMRAEEVGEIIADMRNQVTEELVARHIPERAY
                     AEQWDVAGLEEEVRDVFGLELPVVEWSKEEGIAEEEMKERITEAVTRRTSERAEGFGP
                     EIVRYLEKAILLQTLDHDWREHIIQLEHLRQYVGLRGYGQRDPLNEYKSEALTLFEGL
                     LARLRTDVIRQLMHVQVRTGEPPPVQDPPPLPRMEAHHINPLTGEDEMGAAMPLSTKG
                     VRLRDPGAAVDPGDVSTWGKVSRNATCPCGSGKKFKHCHGALV"
     gene            3238..4578
                     /gene="argJ"
                     /locus_tag="VGM72_00580"
     CDS             3238..4578
                     /gene="argJ"
                     /locus_tag="VGM72_00580"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEY4263785.1"
                     /translation="MGTQGGATIRGKASPKGAVKAKTPVSKPGAAASATTAPHKSPLA
                     PKILANLPPLAGVRLATGEAGIKYRDRTDVLLMVLAPGTQVAGVFTKSKTASAPVDWC
                     RKHLAGGTARVLVVNSGNANAFTGRAGMDSVREVAESAAGVVGCRAQEVFLASTGVIG
                     EPLPHQKITRVLGRLAESGAAGGWRQAAEAIMTTDTFPKLATATAHIDGHRVTISGIA
                     KGSGMIMPDMATMLGFIFTDARLPAGVLQPLLNEAVSRSFNAITVDSDTSTSDTLLLF
                     ATGKGAAHPPISKPSDKRLDQFKDRLNAVLLDLALQIVKDGEGATKLIQVDVTGAQTN
                     ESAKKIALSIANSPLVKTAIAGGDANWGRVVMAVGKAGEPAERDRLKIMFGTHVVADK
                     GERATGYNEALATKAVSGPEVVISSDVGVGKGKATVWTCDLTEGYIRINGSYRS"
     gene            4583..4993
                     /gene="mutT"
                     /locus_tag="VGM72_00585"
     CDS             4583..4993
                     /gene="mutT"
                     /locus_tag="VGM72_00585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HEY4263786.1"
                     /translation="MPLVLVAACVLIDSDGRVLIAQRPEGKTMAGLWEFPGGKIEPGE
                     RPEETVIREMREELGVAIKEACLAPFIFASHSYPEFHLLMPLFLCRRWEGIARAHEGQ
                     KLAWVRPKDLALQSERYPMPPADVPLILMLRDLL"
     gene            complement(4995..5951)
                     /locus_tag="VGM72_00590"
     CDS             complement(4995..5951)
                     /locus_tag="VGM72_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY4263787.1"
                     /translation="MNPTPGEGPGYDAYFPWHAHGLTLAKMSQAPPRIFDSFAYRMRR
                     ARAERMAEDIFLAQDAAGHLAERMGAINRRFTDGLDIHSRARIFPLLAPFAENWVRMG
                     FGWNTPSVIADEEVLPFADGSFDLVTSVLSLHAVNDLPGTLVQIRRALRPDGLFLAAM
                     FGGETLRELRNAFAAAETEILGGISPRISPFADVRDLGGLLSRAGFTLPVADVERTIV
                     RYRDLARLFADLRALGETNVLAERRNKFLSPRILEAVTREYSQRFTDADGRFLATFDI
                     VFLTGWAPHESQQKPLQPGSAKTRLADALGTKEQPAGEKPVP"
     gene            5964..6674
                     /locus_tag="VGM72_00595"
     CDS             5964..6674
                     /locus_tag="VGM72_00595"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEY4263788.1"
                     /translation="MLFPPLCMSCKARLSEPHSLCASCWSAISFIDEPFCARCGIPFD
                     IDPGGETVCGPCHSKPRAFDTARSLLRYDDASKPLVLTFKHGDRLDQAPAFARWLERA
                     GRSLLSETDLIVPVPLHRWRLWRRRYNQAAVISERLARLCARPHDPLALERKRPTPSQ
                     GKMISAKARRRNVLGAFRVPPAKAPSVKGRAILLVDDVFTTGATLEACARALKRAGAV
                     RVDALTLSRVVRPGFSDI"
     gene            6700..6960
                     /gene="grxC"
                     /locus_tag="VGM72_00600"
     CDS             6700..6960
                     /gene="grxC"
                     /locus_tag="VGM72_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004813145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEY4263789.1"
                     /translation="MARIVMYTTQVCPYCVQAKRLLNRKGVAFEEIDISRDPELRRTM
                     VQKAGGRLTVPQIFVDDKYLADCDGMYALDRAGKLDALLNPS"
     gene            6957..7787
                     /locus_tag="VGM72_00605"
     CDS             6957..7787
                     /locus_tag="VGM72_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY4263790.1"
                     /translation="MSERKIKVACVQLRCGDDVAENVGSALKLIREAHKAGAQFIATP
                     ENTNLMAADGGAKLEKTVVEKDDSALPQFAGVAEELGIWLLVGSLGIKVSEGKTANRS
                     YLIGPNGRIAARYDKIHLFDVNLPSGETYRESNTVTPGADAVVASLPWARIGLSVCYD
                     LRFPQLYRALAKEGAEILTVPSAFTETTGKAHWHVLLRARAIENGCFVVAPAQGGTHA
                     NGRKTYGHSLIVGPWGDILAEAGIDPGIVTADLDLEEIASVRARLPSLHHDRPFSAPD
                     "
     gene            7861..8322
                     /locus_tag="VGM72_00610"
     CDS             7861..8322
                     /locus_tag="VGM72_00610"
                     /inference="COORDINATES: protein motif:HMM:NF018391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEY4263791.1"
                     /translation="MIAYNLACSEGHEFEGWFQSGAAYDAQEAQGALACPFCGDAQVK
                     KAIMAPAVRTSVTKAKGKTDLAPTQPGGAPAIDKPDPQKLRQFMAGYRKFIEQNADYV
                     GPRFPEEARKIHYGETEERHIYGESTIGEAREMIEEGIAIAPVPSDPGDLN"
     gene            complement(8319..9065)
                     /gene="ubiG"
                     /locus_tag="VGM72_00615"
     CDS             complement(8319..9065)
                     /gene="ubiG"
                     /locus_tag="VGM72_00615"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: protein motif:HMM:TIGR01983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEY4263792.1"
                     /translation="MAAASSVDPAEVEKFTRLAAEWWDPNGKFRPLHVFNPVRLTFIR
                     ALTAEHFGRDERSLAPFTGLTLLDIGCGGGLLSEPMARMGFHVLGADASEKNVRIAAA
                     HAAASGLALDYRAVTAEALAAEGASFDVILNMEVVEHVADLGGFLESCASLLKPGGLM
                     LVATLNRTLKSLALAKIGAEYVLRWLPAGTHDWNRFVKPADLQRLLEDTGLVVTGLQG
                     VAFDPLRWDWQLSTDTDVNYMMAAMQAATR"
     gene            9191..10414
                     /locus_tag="VGM72_00620"
     CDS             9191..10414
                     /locus_tag="VGM72_00620"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEY4263793.1"
                     /translation="MAKIVMKFGGTSVADIERIRNVAVLVKREVDRGHEVAVAVSAMA
                     GETNKLVAWARELSPLHDAREYDVVVASGEQVSSGLLAIALQSIGVSARSWLGWQIPV
                     RTSSMHSSARIESIDPAVLNQRMAEGQVAVIAGFQGIAPDNRITTIGRGGGDTSAVAV
                     AAALKADRCDIYTDVDGVYTTDPRIVPKARRLERIAFEEMLEMASLGAKVLQTRSVEI
                     AMLHRVPVRVLSTFNPEAGGTLLCDEEDIVEKKPVTGIAYTRDEAKITLLRIPDQPGV
                     AAAIFGPLAEAGVNVDMIVQNVSEDGRRTDLTFTVSRAELDRALDAIRKAAPQIGYKD
                     LISAANVAKVSIIGIGMRAHPGVAQTMFKTLSEKGINIQVISTSEIKVSVLIAEDYVE
                     LAVRALHSAYDLDAA"
     gene            10546..12828
                     /gene="ptsP"
                     /locus_tag="VGM72_00625"
     CDS             10546..12828
                     /gene="ptsP"
                     /locus_tag="VGM72_00625"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEY4263794.1"
                     /translation="MPPQLAAGGPRVLLRRLREIMAERQSAQARLDKLVSVIAANMVA
                     EVCSIYLRRAGNALELFATEGLNRAAVHSTRLKPGEGLVGLVAQSATPLNLSNAPEHP
                     SFSYRPETGEDPFNAFLAVPIVRGERVFGVLTVQNRTQRHYGEEEEEALATIAMVLAE
                     VVAQGTLVDLAEIDEAELSPTRPATFHGEGLAEGLAVGTVFLHQPRVKVDRMIADDPK
                     VELLRLEDALEQLRIAVDAMLESSELDLAGDTREVIEAYRLFAHDQGWRQRLTDAVRS
                     GLTAEAAVERVQDETRARINRTNDAYLRERLHDFDDLARRLMRHLVKTDGGETENLPA
                     QAIVVARTMGPAELLDYGRNRIMALVVEEASQTSHIAIVARSLGIALVGGVEGVADAA
                     RAGDRIVVDSDSGEVHLRPLGEVITAFQAKQGLLAQRVARFAAIRDEPAVTLDGVQVS
                     LNMNAGLLLDLPHLGQSGADGIGLFRTELQFMIGSNMPRLKDQIAFYKQVLDAAGDKD
                     VVFRTLDLGGDKVPSYGRTVREENPALGWRALRIALDRPALLRYQTRALITAAGGRSL
                     RLLLPMVSEVAEFETARGQIGRELDRAKRVGLTLPQSIRLGAMVEVPSLLFVLKQLLQ
                     AADFISIGSNDLLQFIFAADRTNPTVARRYDTLSPAVLNLIRRVVDEGRQGERLSDIS
                     ICGEIAGRPLEAMALVGSGIRSLSMQASMIGPVKMMIRSLDTRLLKPMVDRLCGTAMP
                     SARAELTDFASSHGIPIGRA"
     gene            12968..13909
                     /locus_tag="VGM72_00630"
     CDS             12968..13909
                     /locus_tag="VGM72_00630"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY4263795.1"
                     /translation="MAKVTRLSLDSSGGLERRSLHLREISDDAELPLETVGLDLRKAR
                     QRKGEDLAHIAQVLKIRRGYLDALEESHFDAIPGTAYTIGFVRTYAQYLGLDARNCVE
                     RIKTEIAGRSDTRDGTVQVSSPRERSLPRGGIVFAVLLAIAVIYGVYYLFIAVNRMTS
                     QPVTPVPPRLAAQAGLSPPDDPPLSGPAAPTAEPVPPLPGAEAANGTAQTVAPLPEGH
                     KYGIQTNSRIILLAHKPTRVTVFGPNRRLMLDRQLQPGDSYFVPDTAGLTLSASNGGA
                     LELILDGNTMGFAGQEGLVSEGLSLNPQDVAGHKARG"
     gene            13914..15035
                     /gene="ispG"
                     /locus_tag="VGM72_00635"
                     /gene_synonym="gcpE"
     CDS             13914..15035
                     /gene="ispG"
                     /locus_tag="VGM72_00635"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005107871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEY4263796.1"
                     /translation="MSIRPYRDIQRRKSRKIHVGTVEVGGDAPITVQTMTNTLTSDAR
                     ATIAQIKRVEDAGADIVRVSCPDEDSTKALKAIVRASRVPIVADIHFHYKRAIEAAEA
                     GAACLRINPGNIGSASRVRDVIQAAKDHGCSMRIGVNAGSLEKELLEKYGEPCPEAMV
                     ESAQRHMQILEDNDFFEFKVSVKASDVFLAVAAYQQLAEVCDYPLHLGITEAGGMIPG
                     TVKSSIGMGMLLWSGIGDTIRVSLSAEPEEEIRVGFDLLKSLGLRHRGVNIVSCPSCA
                     RQGFNVIKTVELLEERLKHIATPMSLSIIGCVVNGPGEARETDIGFTGGGAGAGMIYL
                     NGKPVYKLENASLVDHVVELCEKKAAEIEAERNVLEPAQ"
     gene            15111..16187
                     /gene="prfA"
                     /locus_tag="VGM72_00640"
     CDS             15111..16187
                     /gene="prfA"
                     /locus_tag="VGM72_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEY4263797.1"
                     /translation="MIPDAKLERVLDRFQAIEHQLASGHSEEFAKLSKEHSELSPVVN
                     AIRAYKDTRVQLKDSETLLADPASDSELKALAQEESDTLRKKFGALERHVQLLLLPKD
                     SADDSSAIIEVRAGTGGEEASLFAGNLLKMYTRYAQLQGWKTELLSLSESDLGGVKEA
                     VLSTEGEGAYAKLKFESGVHRVQRVPVTEGSGRIHTSAATVAVLPEPEDVDVHIDDKD
                     LRIDVYRASGAGGQHVNKTESAVRITHLPTGIVVAMQEEKSQHKNRAKAMKILKARMF
                     EAERERQANARAAERKGLVGSGDRSERIRTYNFPQGRVSDHRINLTLYKLDRIISGDD
                     LGEVIDALLTEDQASRLAAEEDIA"
     gene            16184..17089
                     /gene="prmC"
                     /locus_tag="VGM72_00645"
     CDS             16184..17089
                     /gene="prmC"
                     /locus_tag="VGM72_00645"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY4263798.1"
                     /translation="MSWESADALLADASAQLAAAGVESPRREAKLLLRHATDVAREDI
                     VVGQLPPLTPQMIARFKTAVRRRIAREPFAYIVGSREFWSLDLAVGPDVLIPRPESET
                     LIEEALQRFPERNAALRAADLGTGSGCLLLAFLSERPNAQGVGVDVSTDALALARCNA
                     EALGLQARVQFQWSDWTENLLGVFDVIFVNPPYISHHELESLEPEILRYEPRAALDGG
                     PDGLDAYRRISSRLAAHLSLQGLAFFELGKGQRDSVEAILAQEGFIAQGTVCDLAGIP
                     RCLVAGLDAAKITPKKELALDTRSG"
     gene            17369..17797
                     /locus_tag="VGM72_00650"
     CDS             17369..17797
                     /locus_tag="VGM72_00650"
                     /inference="COORDINATES: protein motif:HMM:NF025137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HEY4263799.1"
                     /translation="MKRSRGRGRRPQQQNHNRAFDSTGPDVKIRGTAAHVYEKYLQLA
                     RDAGSAGDRVTAENYLQHAEHYYRILMAQGPQTQTGQPGFVPQGQGQQPNVNGNGNGD
                     GRPMNNGEQPAYPQPVPGGSAPSFSLAEAEDDMEEGDEAA"
     gene            17941..>19274
                     /locus_tag="VGM72_00655"
     CDS             17941..>19274
                     /locus_tag="VGM72_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003849026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEY4263800.1"
                     /translation="MDFEKFTERARGFIESAQGLALRSNHQLLSPEHILKILLDDEEG
                     MASNLIQAAGGRPVEVRQQVEIALGKLPKVQGGSGQVFLAPETARVFDLAQELAKKAG
                     DSFVTAERLLQALAMSAGTPSAIALKNAGVTPQNLNAAIGALRQGRTADTATAENSYE
                     ALKKYARDLTETARAGKLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIAE
                     GLALRIVNGDVPESLRDKRLMALDMGSLIAGAKFRGEFEERLKAVLSEITAAQGKVIV
                     FIDELHTLVGAGKAEGAMDASNLLKPALARGELHCIGATTLAEYRKYIEKDAALARRF
                     QPVFVSEPSVADTISILRGLKEKYEVHHGVRITDGALVAAATLSHRYITDRFLPDKAI
                     DLMDEAASRLRMQVDSKPEELDEFDRRIIQLKIEREALRKESDKGSKDRLEKL"
BASE COUNT         3783 a         5705 c         6058 g         3728 t
ORIGIN      
        1 gaaatcaaat ctatcagcaa atttgggatt tggcacgcgc cgtcgcgaga cccaaaactc
       61 agacactttc atccatttgg atgaaagtgt ctggccggat tgagtttttt gaaatgagag
      121 cgcgtgtatt tcgaaggtgc ggcttgcgcg cgcgttcgcg ttgtcagacc agcgcgccgt
      181 ggcagtgctt gaatttcttt cccgaaccgc agggacaggt cgcgttgcgg ctgacctttc
      241 cccaggttga gacatcgccc ggatcgacgg ccgcgccggg atcgcgcagc cggacaccct
      301 tcgttgaaag gggcattgcg gcgcccattt cgtcttcgcc ggtgaggggg ttgatgtggt
      361 gggcttccat gcgcggcaga ggcggggggt cctgcaccgg aggcggttcg cccgtgcgca
      421 cctgcacatg catgagctgg cggatcacat cggtgcgcag ccgcgccagc agaccctcga
      481 acagggtcaa agcctcgctc ttatattcgt tgagcgggtc gcgctggccg tagcctctga
      541 ggcccacata ttggcgcaga tgttcgagct ggatgatgtg ttcgcgccaa tcatggtcca
      601 gcgtctgaag caggatcgcc ttttcgagat aacgcacgat ctcaggaccg aacccttccg
      661 ctctttcgga agtgcgccgc gtcacggcct ccgtgatgcg ctctttcatc tcctcttcgg
      721 cgatgccttc ttccttcgac cactcgacga cgggaagttc gagtccgaag acgtccctca
      781 cttcctcttc caggccggcc acgtcccatt gctcggcgta ggcccgttcg ggaatgtgcc
      841 gcgcgacgag ctcttcagtg acctggttgc gcatatcggc gatgatttcg ccgacctctt
      901 cggcccgcat gatgtcgaga cgctgttcga agatcacctt gcgctggtcg ttcagcacgt
      961 tgtcgaactt caaaacgttc ttgcgcattt caaaattgcg cgcttccact ttctgctggg
     1021 ctttttcgag tgccttgttc acccagggat gagcaatcgc ttcgcctttt tcgagtccca
     1081 gccgctgcag gatggaatcc atgcgctccg acccgaagat gcgcatcaga tcgtcctgca
     1141 agctcaggaa gaatttcgac gcgccgggat cgccttgacg cccggaccgg ccgcgaagct
     1201 gattgtcgat gcgccggctc tcatggcgtt ccgtcccgag aatgaaaagt ccgcccgcgc
     1261 cgatgacctt ctgtttgtcg gcaacgacct cggccttgat ctcggcgatg cggcgcgcgc
     1321 gttcagcttc gtcggtgaca tcggcgagtt ccagccggat gcgcatgtcc ggatggccgc
     1381 cgagctgaat gtcggtgccg cgtcctgcca tgttcgttgc gatggtgacg ccatgcgagc
     1441 ggccggcttg cgccacgata taggcttcct gttcgtggta gcgcgcattc agcacattgt
     1501 gttcgatggg attggatttg gaggagatcg agacgagata ttctccgatc tcgcgatgct
     1561 ccttgccttg tggcgtatcg ccgagcttct gggcccgctc gatcaattcc tcgcccgttt
     1621 cacggatgta tttgcggtcg cgaagtttgg catcgagggt ttcggatttc tcgatcgaca
     1681 ccgtgccgac gaggacgggc tggcccttca tatggcattc ggcgatctgg gtgacgatcg
     1741 cgcgatgctt ctcgtcttcc gtgcgataga cctcgtcgtc cgcatcctga cgcttgaccg
     1801 gcatattggt cggaatctcg atcacgtcga gattgtagat gtccatgaac tcggcagctt
     1861 cggtgagggc cgtgcccgtc atgccggcaa gcttgccgta gagacggaag taattctgga
     1921 atgtgatcga ggcgagtgtc acgttctccg attggatgga tgtgtgttcc ttggcctcca
     1981 gcgcctgatg caggccttcg gaataacgcc gtccttccat catgcgcccg gtgaattcgt
     2041 cgatgatgac gaccttgtcg tccttgacga tgtaatcctt gtcgcgctgg aacagcttgt
     2101 gggcgcgcag cgcctgattg acgtgatgga cgacgacgat gttgttgatg tcgtagagat
     2161 cgccttcttc gagcgcgtcc acgcttcgca gaagctcgac catgtgctca ttgccctgtt
     2221 cggtcaacga gacctggcgc tgcttttcgt cgagctcgaa gtcctccgca acgaggcctg
     2281 aaaggatcga gtcgaccttc ttgtacagtt ccgattgatc ttcggtcggt ccggaaatga
     2341 tcaggggcgt gcgcgcttca tcgaccagga tggagtcgac ctcgtcgacg atggcgtaat
     2401 ggtggccgcg ctgcgccatc tgatcgagat tgtatttcat attgtcgcgc agatagtcga
     2461 agccgaactc gttgttcgtg ccgtaggtca catcggcggc atagttgacg cggcgctcct
     2521 catcggtgag gccgtgcacg atggttccga ccgagaggcc gaggaagttg tagatctgtc
     2581 ccatccactc cgcgtcgcgc gtggcgaggt agtcgttcac ggtgacgaca tgcacaccct
     2641 tgccttcgag agcgttgagg tagaccggca gggtggccac gagcgtcttg ccttcgcccg
     2701 ttttcatctc ggcgatccgg ccttcgtgca agaccatgcc gccgatcagc tggacgtcga
     2761 aatggcgctg gccgagggtg cgtttggcgg cttcgcgtac cgccgcgaac gcctccggca
     2821 gaaggtcgtt cagcgtctcg ccattctgga accgctgctt gaaagtggcc gtcagggcca
     2881 gaagctcggc atcggatttt ttttcgaatg cgggctcaag cgcgttgatc ttggcgaccg
     2941 tggccaattt gggcttgacc ttccggtcgt taacggaccc gaaaaggccg gtcagtgcgt
     3001 tcagaggacg ctggatgaaa ctgtcgagac tgagcatcaa ttcctcgtgc tgcccaggga
     3061 ggaacggccg gaaaaccgct cgaggagcat ttcccgcgca ttctggcccc tttaaggccg
     3121 ggggagagat aagaaccgca cccccccttg tcaattcgcg taactcgggc ctaatcggcc
     3181 tttccgggtt gtcataaatg agtcgctggc cggatatggc aatttaagag aaaagccatg
     3241 gggacgcagg ggggtgcgac gatacggggc aaggcctcgc cgaaaggcgc ggttaaggcc
     3301 aaaacaccgg tttcaaagcc gggcgctgct gcttctgcca cgactgcgcc gcacaaatcc
     3361 cccttggcgc ccaagattct cgccaatctg cctccgctcg ccggggtgcg tctcgccacg
     3421 ggcgaggcgg gcatcaaata tcgcgaccgg accgatgtcc ttctgatggt gctggcgccc
     3481 ggcacgcagg tggctggggt cttcaccaaa tcgaagacgg cgtccgcgcc agtggattgg
     3541 tgccggaagc atctggctgg cggaaccgcg cgcgtcctgg tggtgaacag cggcaatgcg
     3601 aacgccttca cgggaagggc cggaatggac agtgttcgcg aggtggcgga atcggcggct
     3661 ggcgtggtcg gctgccgggc gcaggaggta ttcctggcct cgactggtgt catcggagaa
     3721 ccgctgccgc accagaaaat caccagggtc ctgggacggc tcgcggaatc cggggcggcc
     3781 ggcgggtggc gccaagccgc cgaagccatc atgacgacgg acaccttccc gaagctcgcg
     3841 accgcaacgg cgcacatcga tggccatcgg gtgacgatca gcggtatcgc caagggatcg
     3901 ggcatgatca tgcccgacat ggcgaccatg ctgggattca tcttcacgga tgcgcgtttg
     3961 ccggccggcg tgctgcagcc gcttctcaat gaggcggtct cccgctcctt caacgccatc
     4021 acggtcgaca gcgatacctc gaccagcgac acgctgctgc tgttcgcgac aggcaagggc
     4081 gccgcccatc ctcccatatc gaagccgagc gacaagcgcc ttgaccagtt caaggaccgg
     4141 ctgaacgcgg tgctgcttga tctagcgctg cagatcgtca aggacggcga gggggcgacc
     4201 aagctgatcc aggtggatgt gacaggcgcc cagaccaatg aatcggcaaa gaagatcgcc
     4261 ctttccatcg ccaattcgcc tctggtgaag acggcgatcg ccgggggcga tgccaattgg
     4321 ggccgggtgg tgatggccgt gggcaaggca ggcgaaccgg ccgaacgcga cagactgaag
     4381 atcatgttcg gcacccatgt cgtcgccgac aagggcgaaa gggcgaccgg ctataatgag
     4441 gcgctcgcaa ccaaagcggt ctccgggccg gaagtcgtga tttcctcgga tgtgggtgtg
     4501 ggcaaaggca aagccaccgt ctggacctgc gacctgacgg aaggttacat ccgcatcaac
     4561 ggctcctatc gcagttgaac ctttgcctct cgtcctcgtc gcagcctgcg tgcttatcga
     4621 ttccgacgga cgtgtgctga tcgctcagcg ccccgaaggc aaaaccatgg caggtctgtg
     4681 ggaatttccc ggcggcaaga tcgagcccgg tgaacggcca gaagaaaccg tgatccgcga
     4741 aatgcgcgaa gaactcggtg tggccatcaa ggaagcgtgc ctcgcgcctt tcatcttcgc
     4801 ctcgcacagc tatcccgagt ttcatctctt gatgccgctg ttcctctgcc ggcgctggga
     4861 gggcatcgcg cgcgcccatg aaggacaaaa gctcgcctgg gtacgcccca aggatctggc
     4921 gcttcagtct gagcgctacc ccatgccgcc ggcggatgtg ccgctgatcc tgatgttgcg
     4981 cgacctgctg taagtcaggg cacgggtttc tctcccgctg gctgttcttt agtgccgagt
     5041 gcgtcggcaa ggcgtgtctt tgccgatcct ggctgaagcg gtttctgctg gctctcatgc
     5101 ggtgcccagc cggtcaggaa cacgatgtcg aatgtcgcga ggaaacgtcc atcggcatcg
     5161 gtgaagcgct gggaatattc gcgcgtcacc gcttccaaga tgcgcggcga caggaatttg
     5221 tttctccgct ccgccagcac attggtttcg ccaagcgcgc gcaaatccgc gaacagccgc
     5281 gcgagatcgc gatagcgcac gatcgtgcgc tccacatcgg caacgggaag ggtgaagccc
     5341 gcgcggctga gtagcccgcc gagatcacgc acatctgcga agggcgagat tcgcgggctg
     5401 ataccgccca ggatttcggt ttcagcggcg gcaaacgcgt tcctcaactc ccgcaaggtc
     5461 tcgcccccga acatcgccgc caggaacagg ccgtccggcc gcaacgcgcg ccggatttgt
     5521 accagtgtgc cgggaagatc gttgacggcg tgcagcgaca gcacgctcgt caccagatcg
     5581 aagcttccat ccgcgaacgg cagaacctct tcatcggcaa tcacggacgg cgtgttccag
     5641 ccaaacccca tccggaccca gttttccgcg aaaggggcca agagcggaaa aatccgtgcg
     5701 cgggaatgga tgtcgagccc atcggtgaaa cgcctgttga tggcacccat ccgctcggcc
     5761 agatgccccg ccgcgtcctg ggccagaaaa atgtcttcgg ccatccgttc ggcgcgcgcg
     5821 cggcgcatgc ggtaggcgaa cgaatcgaaa atgcgtggcg gggcctggct catcttcgct
     5881 agagttaggc catgagcatg ccaagggaaa taagcgtcat agccgggtcc ttcgcccggc
     5941 gtgggattca tggtgttctc gacgtgctgt tcccacccct ttgcatgtca tgcaaggcgc
     6001 gcctgtccga acctcattct ctctgcgcgt cgtgctggag cgcgatctcg tttatcgatg
     6061 agcctttctg cgcgcggtgt ggcatcccat tcgacatcga tccgggcggc gagacggtgt
     6121 gcggcccctg ccacagcaaa ccacgcgcct tcgacacggc ccgctcgctc ctgcgttatg
     6181 acgacgcatc caaaccgctt gtcctcacct tcaagcacgg cgaccgtctg gatcaggcgc
     6241 ctgcttttgc gcgctggctg gaacgcgccg gccggtcctt actgagcgaa accgacctga
     6301 tcgttccggt gccgctgcat cgctggcggc tgtggcggcg ccgctacaac caggccgcgg
     6361 tgatctctga acgtctcgcg cggctctgcg cgcgtcccca cgatcccctg gcattggaac
     6421 gaaaacgtcc cactcccagc cagggtaaga tgatttcggc caaggcgcgg cggcgcaatg
     6481 tgcttggagc tttccgggtg cctccggcga aagccccaag cgtcaaaggc cgcgcgatcc
     6541 tgctggtgga cgacgtgttc accacaggcg ccacgctcga agcctgtgcc cgggccttga
     6601 agcgggcagg ggccgtccgg gtcgacgcgc tcactttgtc gcgcgttgta aggcccggct
     6661 ttagcgatat atgagggagt gtggattaga gttggatgta tggctagaat cgtcatgtat
     6721 acgacgcagg tttgtcccta ttgcgtacaa gcgaagcgat tgctcaacag gaagggtgtc
     6781 gctttcgagg aaatcgacat atcgagggat cccgaactga ggcgcaccat ggtgcagaaa
     6841 gccggcgggc ggctcaccgt tccgcaaatc ttcgtcgacg acaaatatct cgccgattgc
     6901 gatgggatgt acgcactgga tagggcaggc aagctcgacg cgctgctcaa cccgtcatga
     6961 gcgaacgcaa gatcaaagtc gcttgcgtgc agctgcgctg cggcgacgat gtcgccgaga
     7021 atgtcggcag tgcgctcaag ctgatccgtg aggctcataa agcaggcgct cagttcatcg
     7081 cgacgcccga gaacaccaat ttgatggcgg ccgatggcgg cgccaaactg gaaaagacgg
     7141 ttgtggaaaa ggacgattcc gccctgccgc agttcgcagg cgtggcggag gaactgggca
     7201 tctggttgct ggtgggctcc ctcggcatca aagtgtcgga gggcaagacc gccaatcgct
     7261 cctacctcat aggacctaac gggcgcatcg ccgcgcgcta tgataagatt cacctcttcg
     7321 acgtgaacct tccctccggc gagacctatc gcgaatccaa caccgtaact cccggtgcgg
     7381 acgcggtggt ggccagcttg ccttgggcgc gcataggttt gtccgtctgt tacgatctgc
     7441 gcttcccgca gctctatcgg gccttggcaa aagagggcgc cgaaattctc accgtgccat
     7501 cggctttcac cgaaaccacg ggcaaggccc attggcatgt cctgctgcgc gcgcgcgcga
     7561 tcgagaatgg ctgcttcgtc gtggcgcctg cgcaaggcgg tacgcatgcg aatggacgca
     7621 agacctacgg gcacagcctc atcgtcggcc cctggggcga tatcctggcc gaagcgggaa
     7681 tcgatccggg catcgtcacg gcggatctgg atttggaaga aattgcttct gttcgcgcgc
     7741 gccttccctc cctgcaccat gaccgcccgt tcagtgctcc ggattgagac gcggtttgcg
     7801 taaaccaatt tcagcaatcg tccatggtga aatgggtctg cattcccaag aggacctcgc
     7861 gtgatcgcgt ataatcttgc ctgttccgag ggccatgaat tcgaaggctg gttccagagc
     7921 ggcgccgcct atgatgcgca agaagcgcag ggtgcgcttg catgcccgtt ctgcggcgat
     7981 gcccaggtaa aaaaggcgat catggcgcct gcggtcagga cctccgtcac caaggcgaag
     8041 ggcaagacgg atctcgcgcc cacgcagccc gggggcgcgc cggccatcga caagcctgat
     8101 ccccagaagc tgcgccagtt catggccggc tatcgcaaat tcatcgaaca gaacgccgat
     8161 tatgtcgggc cgcgatttcc cgaggaggca cgcaaaatcc actacgggga gacggaagaa
     8221 cgccacatct atggagaaag cacgatcggt gaggcgcgcg agatgatcga agaaggcata
     8281 gcgatcgcgc ccgtgccgtc ggatcctggc gatttgaatt agcgagtcgc tgcctgcatc
     8341 gcggccatca tgtaattcac atccgtatcg gtggagagct gccaatccca gcgtaaggga
     8401 tcgaaggcca cgccctgcag gccggtgacc accagtccgg tatcctccaa aagacgttga
     8461 aggtcggcgg gcttcacgaa tcgattccag tcatgggtgc cggccggcaa ccagcgcagc
     8521 acatattcgg cgccgatttt ggccaaggcc aggcttttga gcgtgcggtt cagggtggcg
     8581 accagcatca agccgccggg cttcagcagg ctcgcgcaag attccagaaa tccgccgaga
     8641 tcggcgacat gttcgaccac ttccatattg aggatgacat cgaagctggc gccctcagcg
     8701 gcgagggcct ctgccgtgac cgcgcggtaa tccaaagcca gccccgaggc agcggcatgg
     8761 gcagcggcta tcctcacatt tttctccgag gcatccgcgc ccagcacgtg aaagcccatg
     8821 cgcgccatgg gctcggataa gaggcccccg ccgcagccaa tgtcgagcag tgtcaggccg
     8881 gtgaacggcg ccaggcttcg ttcgtcccgg ccaaaatgtt ccgcggtcaa agctcggatg
     8941 aacgtcaagc ggaccggatt gaaaacatgc agaggccgga acttgccgtt cgggtcccac
     9001 cactcggctg cgagccgtgt gaatttttcg acctcggccg ggtccacgga ggaagccgcc
     9061 gccacccttg aggcgtcccc ggcggcgttt cgaggctggg atgttgtcac gatgcttcca
     9121 ttggatgttg agccggacga gcctcacgtc tatgtatgac ccgcaaaacc tgcccgcacc
     9181 agacccccaa atggccaaga tcgtcatgaa attcggcggc acatcggtgg ccgatatcga
     9241 gcgtattcgc aatgtggcgg tgctggtcaa gcgcgaggtg gaccgtggcc atgaggtcgc
     9301 cgtggcggtt tcggccatgg ccggagagac caacaagctg gttgcctggg cgcgcgaatt
     9361 atctcctctg cacgatgcac gcgagtatga cgtggtggtg gcttcgggtg agcaggtttc
     9421 ttccgggctt ctcgccatcg cgcttcaatc catcggtgtc agcgcacgct cctggctagg
     9481 ttggcagatt cccgttcgca cgtcgtccat gcacagctcg gcccgaatcg agtccattga
     9541 ccccgccgtc ctgaaccagc gtatggcgga agggcaggtt gccgtgatcg cgggtttcca
     9601 gggcatcgct cccgacaatc gcatcaccac catcgggcgc ggcggcggcg atacgagcgc
     9661 agtggcggtt gctgccgcgc tcaaggcgga ccgctgcgac atctatacgg atgtggacgg
     9721 cgtctacacg accgatccgc ggatagtgcc caaggcgcgg cgcctggagc gaatcgcctt
     9781 cgaagaaatg ctcgagatgg cttcgctcgg tgccaaagtg ctccagaccc gctctgttga
     9841 gatcgccatg cttcatcgtg tccccgtgcg ggttctttcg accttcaatc ccgaagcggg
     9901 cggcacactt ctttgcgacg aggaagacat cgtggaaaag aaacccgtca ccggcatcgc
     9961 ttacacgcgc gacgaagcca agatcacgct tctgcggata ccggatcagc ccggcgtggc
    10021 tgccgccatt tttggaccgc tcgccgaggc cggggtcaac gtcgacatga tcgtgcagaa
    10081 cgtgtcggag gatggccgcc gcacggacct gacgttcacg gtttcgcgcg ccgaactgga
    10141 ccgcgccctc gatgcgatcc gcaaggcagc gccgcaaatt ggctacaagg atctgatctc
    10201 cgcggccaac gtcgcgaaag tttcgatcat cggaatcggc atgcgtgcac accccggggt
    10261 cgcccagacc atgttcaaga cgctgtcgga aaagggaatc aatattcagg ttatctccac
    10321 ctccgaaatc aaggtcagcg tcctcattgc cgaagattat gtggaactcg cggtgcgggc
    10381 gttgcattcc gcgtacgatc tggacgcggc ataatttatg ctaactgata cgctgcgacc
    10441 gtcgcgcatc agggaattga tgagattgcg cgtactcgcg aacgagcatg cccagcagtt
    10501 gagcaacatg atcgaattac gacgcatggc agggaagccc atctgatgcc gccccaactc
    10561 gcagcgggcg gccccagggt tcttctgcgc cgcctgcgcg agatcatggc ggagcggcag
    10621 agcgcgcaag ctcggctcga caagctggta tcggtgatcg ccgccaatat ggtggccgaa
    10681 gtgtgctcga tttatctgcg ccgggcaggg aacgcgcttg agctgttcgc caccgaagga
    10741 ttgaaccgcg cagcggtgca tagcacgcgc ctgaaacctg gcgaggggct ggtgggcttg
    10801 gtggcgcaaa gcgccacacc gttgaacctt tccaatgcgc cggagcatcc tagcttctcc
    10861 taccggcccg aaacaggcga agacccgttc aacgccttcc tggcggttcc gatcgtgcgg
    10921 ggtgagcgcg tctttggcgt gctcacggtg cagaatcgta cgcagcgcca ttatggcgag
    10981 gaggaagaag aggcccttgc caccatcgcc atggttctgg cggaagtcgt ggcgcaaggc
    11041 acgctggtcg atcttgccga gatcgacgaa gccgagttgt cgccgacgcg ccctgcgaca
    11101 ttccacggtg aggggttggc ggaaggtttg gctgtcggca cggtttttct tcatcagccg
    11161 cgcgtgaaag tcgaccgcat gatcgcggac gatcccaagg tggagttgct gcgtctggaa
    11221 gacgcgctgg aacaattgcg catcgctgtg gatgcgatgc tcgaatcttc cgaactcgac
    11281 ctcgccggcg acacgcggga ggtcatcgaa gcctaccgct tgttcgctca tgaccagggc
    11341 tggcggcagc gcctgactga cgcggtgcgc tcgggcctta cggccgaagc ggcggtggag
    11401 cgcgtgcagg acgaaacgcg cgcccgtatc aaccgcacca acgacgcata tttgcgcgag
    11461 cgtctgcacg atttcgacga cctcgcccgg cggctcatgc gccacctcgt gaagaccgat
    11521 ggcggcgaaa ccgaaaacct gcccgcgcag gccattgtgg ttgcgcgaac catggggccg
    11581 gcggagcttc tggactacgg ccgcaatcgc atcatggcgc tggtggtgga ggaggcctca
    11641 cagacgtcgc acatcgcgat cgtggcgcgc tcgctcggca tcgcactcgt cggcggggtt
    11701 gaaggcgtgg ccgatgccgc acgggcgggc gatcgcattg tcgtggattc ggacagcggc
    11761 gaggttcatt tgcgcccgct tggcgaggtg ataacggcat ttcaggccaa acaaggcctt
    11821 cttgcgcagc gcgtcgctcg tttcgcggcc attcgcgatg aaccggctgt tacgctcgat
    11881 ggcgtgcagg tcagcctgaa catgaatgcc ggcctgctgc tcgatctgcc gcatctcgga
    11941 caatccggcg cggatgggat cggactcttc cggaccgaac tgcaattcat gatcggctcc
    12001 aatatgccgc gtctgaagga tcagatcgct ttttacaagc aggtgctgga tgccgcgggt
    12061 gataaggacg tggtcttccg gacactcgat ctgggcggcg acaaggtccc gagttacggc
    12121 cggacggtac gtgaggagaa tccggcgctc ggctggcggg cgttgcgcat cgcgctcgat
    12181 cgcccggcac tgctgcgtta tcagacgcgt gcgctgatca ccgcggccgg cgggcggagt
    12241 ttgcgcctgc tcctgccgat ggtgagcgaa gtggcggaat tcgaaaccgc ccgcggacag
    12301 atcgggcgcg agctggaccg tgccaaacgg gtgggactta cgctgccgca aagcatccgg
    12361 ctgggcgcga tggtggaggt tccctcgctg ctattcgtgt tgaagcaact cctgcaagct
    12421 gctgatttta tttccatagg gtcaaacgat ctcctgcaat tcatattcgc tgcggaccgc
    12481 accaatccca cggtggcgcg gcgctacgat acgctttcgc cggcggtgct gaacctgata
    12541 cggcgcgtcg tggacgaggg aaggcaaggt gaacggttgt ccgatatctc gatctgtggt
    12601 gagatcgcag gccgtcccct ggaggccatg gctttggtag gctccggcat caggtcgctt
    12661 tcgatgcagg cctccatgat cggaccggtc aagatgatga tccgaagtct cgatacgcga
    12721 ttgcttaagc caatggtcga caggctgtgc ggcacggcga tgccgagcgc gcgggccgaa
    12781 ctgaccgatt tcgccagcag tcatggcatt cccatcggac gtgcctaagc tggcttgccg
    12841 ggacttctcg ggttgtggca aaggcaagct tctggtttaa ttgagagact caggatccga
    12901 ttggcaaaag aacttcggac ggccgagatt tttcggcgta gtgaagctca gcggcacagc
    12961 aggcggcatg gcaaaggtaa cgcgactttc gctggacagt agcggtgggt tggaacgccg
    13021 cagcctccat ttgcgggaga tttccgacga tgcggaactg ccgctggaga ccgttgggct
    13081 ggacctgcgc aaagcccgcc agagaaaggg cgaggatttg gcgcatattg cccaagtcct
    13141 gaagattcga agggggtatc tcgacgcgtt ggaagaaagc catttcgacg cgattccggg
    13201 aaccgcttac acgatcgggt tcgtgcgcac ctatgcccaa tatcttggat tggacgcgcg
    13261 caattgcgtc gagcggatca agacggaaat tgcaggacgc agcgacacgc gtgatggaac
    13321 agtgcaggtt tccagcccgc gtgagcgcag cctgcctcgc ggcgggatcg tttttgccgt
    13381 gctgctcgcc attgccgtca tctatggcgt ttattacctt ttcatcgccg tcaaccgcat
    13441 gacgtcgcag ccggtcacgc cggtcccgcc gcggctcgcg gcccaggcgg gtttaagtcc
    13501 gcccgatgat cctcccttgt cagggccagc ggcaccaaca gcggaacctg taccgccgtt
    13561 gccaggggcc gaagccgcca acggcaccgc ccagaccgtc gcgccgcttc cagaaggaca
    13621 taaatacggc attcaaacca attcgagaat cattttgctc gcgcataaac caacgcgcgt
    13681 gacggtgttc ggcccgaacc ggcgtctcat gcttgaccgg cagctgcagc ccggcgattc
    13741 ctactttgtc cccgatacgg cgggcctgac gctctccgct tcgaatggcg gagccttgga
    13801 actgatactc gacggcaata cgatgggttt cgcaggccag gaaggccttg tgtccgaagg
    13861 tctgtcactt aatccgcagg atgtcgcggg gcacaaggcg cgcgggtaag aacatgagca
    13921 ttcgaccgta tcgcgatatt cagagacgaa aatcacggaa gatccatgtc ggcacggtcg
    13981 aggtgggcgg cgatgcgccc atcaccgttc agacgatgac caacacactg acgtccgatg
    14041 cgcgcgccac catcgcgcag atcaaacgcg tcgaggacgc cggcgccgat atcgtgcgcg
    14101 tctcctgtcc cgatgaggat tccaccaagg cgctcaaggc gatcgtcagg gcgtcacggg
    14161 ttcccatcgt ggccgacatc catttccact acaaacgcgc catcgaggca gcagaagccg
    14221 gtgcggcgtg cctgcgcatc aatccaggca atatcggctc cgcgagccgc gtgcgcgatg
    14281 tcattcaggc ggccaaggac cacggctgtt ccatgcgcat cggcgtgaat gccggttcct
    14341 tggaaaagga actgctggag aaatatggcg agccgtgtcc ggaagctatg gtggaaagcg
    14401 cgcagcgcca catgcaaatc ctcgaagaca atgatttctt cgagttcaag gtcagcgtga
    14461 aggcatcgga cgtattccta gccgttgccg cttatcagca actggcggag gtttgcgatt
    14521 atccgttgca tctgggcatt accgaggctg gcggcatgat tccggggacg gtcaaatcct
    14581 ccatcggcat gggtatgctg ttatggtcgg gcatcggaga caccatccgc gtctcgctgt
    14641 ccgccgagcc cgaagaggaa atccgcgtcg gcttcgatct gctcaaatcc cttggcctgc
    14701 ggcaccgagg cgtcaacatc gtgtcgtgtc cttcttgtgc gcggcagggg ttcaacgtca
    14761 tcaagaccgt cgagcttctg gaagaacgcc tgaagcatat cgccacgccc atgtcgcttt
    14821 cgatcatcgg ctgtgttgtg aatgggccgg gcgaggcccg tgagaccgac atcggtttca
    14881 caggcggcgg cgcgggtgcc ggtatgatct atctcaacgg caagcccgtt tataagcttg
    14941 agaatgcgag cctcgtcgat cacgtggtcg agctgtgcga gaagaaggcc gccgaaatcg
    15001 aagccgagcg caacgtcctc gaaccggcac aatagcgatg ttcctggcct cgttattccg
    15061 ggcaagcggt aagctggcgc cttacgcgct gcgatgacgc attctaggca atgattcccg
    15121 atgccaaact ggaacgcgtt ctggatcgct ttcaagcgat cgagcatcaa ctcgcgtccg
    15181 gccactcgga agaattcgcg aagctttcga aagaacattc ggaactttct cccgtagtga
    15241 atgcgatccg cgcctataag gacacacgcg tccagctcaa ggacagcgaa acattactgg
    15301 cggatccggc ttccgattcc gagctgaagg cgttggcgca ggaagaatcc gatacgctgc
    15361 gcaagaaatt cggcgcgctt gagcgtcatg tgcaattgct gctgctgccg aaggattccg
    15421 ccgacgattc cagcgcgatc attgaagtcc gcgctggaac gggtggggag gaggcttcgc
    15481 tgtttgccgg caatcttctg aaaatgtaca cgcgctatgc ccaattgcag ggatggaaga
    15541 ccgagttgct gtcgctcagc gaatccgatc ttggcggcgt gaaagaagcc gtcctcagca
    15601 ccgagggtga gggcgcctat gccaagctca aattcgagtc cggcgtccat agggtgcagc
    15661 gcgtgccggt gacggaaggt tcgggccgca tccacacgtc ggctgcgacc gtcgcggtcc
    15721 tgccggaacc tgaagacgtc gacgttcata tcgatgacaa ggatttgcgc atcgacgtct
    15781 acagggcgag cggcgccggc ggacagcacg tcaacaagac cgaatcggcg gtgcggatca
    15841 cgcaccttcc caccggcatc gtggtggcga tgcaggagga gaaatcgcaa cacaaaaacc
    15901 gggccaaggc gatgaagatc ctcaaagccc gcatgttcga agccgaacgc gagcgtcaag
    15961 ccaatgcacg cgccgccgag cgcaaaggac tggtcggttc cggagaccgg tccgagcgca
    16021 tccgcaccta taattttccg caaggacgcg tgtccgacca tcggatcaat ttgacgctct
    16081 ataagcttga tcgaatcatc tcgggcgatg atttgggcga ggtcatcgac gcgctcctga
    16141 ccgaagatca ggcgagccga ttggcagccg aggaagatat cgcgtgagct gggaaagtgc
    16201 cgatgcactc ctggctgacg cgagcgcaca attggcggcc gccggcgtcg aatcgccgcg
    16261 acgcgaggct aagcttttgc tccgtcatgc aacggacgtc gcccgggaag acatcgtagt
    16321 gggccagttg ccccctctga cgccgcaaat gatcgcacgt ttcaagaccg cggtacgccg
    16381 gcgcatcgcg cgcgaaccat ttgcgtacat tgtaggatcg cgcgaatttt ggagcctcga
    16441 tctcgcggtt ggcccggatg tgctgatccc gcgtcccgaa tccgaaacct tgattgagga
    16501 ggcgttgcag cgtttcccag agcggaatgc ggcattgcgc gcagccgatc ttggaaccgg
    16561 atcggggtgc ctcctgctcg cctttctttc cgagcggccg aatgcccaag gcgtgggtgt
    16621 cgatgtctcc acagacgcac tggccttggc ccgctgcaat gccgaagctc tggggttgca
    16681 ggcgcgcgtc caattccaat ggagcgattg gacggaaaat cttctgggtg tgttcgatgt
    16741 gatcttcgtt aacccgccct atatcagcca tcacgaattg gagagcttgg aaccggagat
    16801 cctacgctac gagccgagag ccgcactcga cggtgggccg gacgggctcg atgcataccg
    16861 ccgcatttcc tcgaggctcg cagcgcattt aagcctgcaa ggattggctt ttttcgagct
    16921 ggggaagggt caaagggatt cggtggaagc tattttggcg caggaagggt tcattgccca
    16981 gggaaccgtt tgcgacctcg ccggtattcc ccgttgtttg gttgccggct tggatgcagc
    17041 aaagattacg ccaaaaaaag aattggcatt ggacacacga agcggctagg gtttcgaata
    17101 ctggggaaag acgaaggtgc cggccgccgt acaacggaag agagccggag gaagcgcaaa
    17161 cgaaagcgcc gtccttcgtg acgtgagagc tcggaagatc gcgcgaacca taaaataaaa
    17221 tgggtaggaa tcgtgcgcgt ccaggctgag gaaaaacgcg tttgggtatg acaacacaga
    17281 ttcgcatgag gtgggacatt cccatatgcg gcccgaacca accgttcggc tgcgctaacc
    17341 acacaaagac caatatcgag gggtagacgt gaaacgttca cgcggacgcg gcagacggcc
    17401 gcaacagcaa aatcataatc gggcattcga ttcgactggg cccgacgtga agatccgtgg
    17461 caccgcggct catgtctacg aaaaatatct ccaactcgcg cgcgacgcgg gttcggccgg
    17521 agaccgggtg acggcggaga actacctcca gcatgcggaa cattattatc gcatcctgat
    17581 ggcgcaaggt ccgcagaccc agacaggcca gcctgggttc gtgccgcaag gtcaggggca
    17641 gcagcccaac gtcaatggga acggcaatgg cgatggccgg cctatgaaca atggcgagca
    17701 gcccgcctat ccgcagccgg taccgggcgg cagcgcgcca tccttcagcc tggccgaagc
    17761 ggaagacgac atggaagagg gcgacgaagc cgcgtaattg cggccttacg gccgagcttc
    17821 gcccttgtct tgagccaaat caaagccaca taagccgttg gtacggtgcc attgggccgt
    17881 acggaatcgg cgtgtcttcg cgcctgaagg ggcgaaggta cggcgaaaag gagcggcttt
    17941 atggatttcg aaaagtttac cgaacgtgcc cgcggattta tcgaatcggc ccaagggctt
    18001 gcgctgcgat ccaaccacca actcttaagc ccggaacaca ttctgaaaat cctcctcgat
    18061 gacgaggagg gcatggcgtc taatctgatt caggctgccg gcgggcgacc cgtggaagtc
    18121 cgtcagcagg tcgagattgc gcttggcaag cttcccaagg tgcagggcgg gagcggccag
    18181 gtctttctcg cgcccgagac ggcgcgtgtg ttcgaccttg cgcaggaatt ggcgaagaag
    18241 gcgggcgatt ccttcgtgac ggccgagcgg ctgttgcagg ctctggcgat gagcgcgggc
    18301 acgccttcgg caattgcgct gaagaatgcg ggcgtgactc cgcaaaacct caacgcggcc
    18361 atcggcgcat tgcgtcaggg ccgaaccgcg gacacggcga cggcagagaa ttcctatgag
    18421 gcgttgaaga aatatgcccg cgatctgacc gagaccgcgc gtgcgggcaa gctcgatccg
    18481 gtcattgggc gcgacgagga aatccgccgc accatccagg tactgtcgcg gcgcaccaag
    18541 aacaatccgg tgctgatcgg agagcccggc gtcggcaaga ccgcgatcgc ggaaggtttg
    18601 gcgctgcgca tcgtcaacgg cgatgtcccc gaatccttgc gcgacaagcg gctgatggcg
    18661 ctcgacatgg gctcattgat tgcgggcgcg aaattccgcg gcgagttcga ggagcggctg
    18721 aaagccgtgt tgtccgagat cactgccgcg caaggcaagg tgatcgtgtt catcgacgag
    18781 ctgcacaccc ttgtcggggc cggcaaggcc gaaggcgcga tggatgcgtc caatcttttg
    18841 aaaccggcgc tcgcgcgcgg cgagctgcat tgcattggcg cgaccacact cgccgagtat
    18901 cgcaaatata tcgaaaagga tgcagctctg gcgcggaggt tccagccggt gttcgtgtcc
    18961 gaaccttccg tcgcggatac gatctcgatc cttcgtgggc taaaggagaa atacgaagtt
    19021 caccacggcg tacgcattac cgatggagcg ctcgtcgcgg cggcgactct ttcccaccgc
    19081 tacatcacgg accggtttct tcccgataaa gccatcgatc tgatggacga agccgcaagc
    19141 cgcctacgca tgcaggtcga ttccaagccg gaggagctcg acgaattcga ccggcgcatc
    19201 atccagctca agatcgagcg tgaagcgctg aggaaggaaa gcgacaaggg ttcgaaagac
    19261 cgccttgaga agct
//
LOCUS       DASYEG010000011         4011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1598930, whole genome shotgun sequence.
ACCESSION   DASYEG010000011 DASYEG010000000
VERSION     DASYEG010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598930"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VGM72_00660"
     CDS             complement(<1..371)
                     /locus_tag="VGM72_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263801.1"
                     /translation="MRRTLVVVTLLSFLTGCTLLRPNTMPAAPKQNLFVVFFVPGTSQ
                     LAREAEQIVRQAAGAALQSKMSKIEIAVPRDTPGGVPLVEERVTAIQNILSASHVEQK
                     FLAPTVLLETAQIPAAVDRAEL"
     gene            612..1985
                     /locus_tag="VGM72_00665"
     CDS             612..1985
                     /locus_tag="VGM72_00665"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY4263802.1"
                     /translation="MSAVAQNDVAPDLRDRPPVAAPTSKIETGAIPIVLSESQEVDNF
                     AAGLMQGLMAGSRVRGIALVVVKDDHVMLQRTAGAIAPDMRFKGGGLSDVFAAVAVMQ
                     QVERGRLTVTADISRALGESNPHGVTLEQVLTFQGGDSSLPVRAVEKASGTGWPDYFA
                     KAIAQPLGMTATAVRGAELETNLTDMSHFAIALVNDGVFQNGRILMPASVESMQSTHY
                     TSYPLLPGAAYGFTEMRRHAWRALQHDGAAGDFASRLVIVPEAKLGYFIVVQGAEGPE
                     FWRALDNGLFDRLLPAQGHDVRATPAGAPAPTQADAEAMAGPYESIRTLATDAAPLKL
                     GGRFTARAGGSAELILTGSPSATLTPRPGGYWGSADGNLIAVPVMGNLMLSTGAYGRL
                     ALYKRPGLYLALALLALIAAGSLFIFERRGKQPRRLPMSPVLGLAGAGVIFALLSAVL
                     WLLSPGA"
     gene            2159..2782
                     /locus_tag="VGM72_00670"
     CDS             2159..2782
                     /locus_tag="VGM72_00670"
                     /inference="COORDINATES: protein motif:HMM:NF009205.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEY4263803.1"
                     /translation="MTRILPVSLDDDDEKEKKENEGMKPEQPMAPVQMALFKARTVLI
                     FGEIDMKVAERVCGQLIAYAADGKGDIRVIINSPGGHVESGDTIHDMIRFCGPRVKMI
                     GTGWVASAGAHIFLGAEKENRFCLPNTRFLLHQPLGGVTGQASDITIEAEEIIKMRER
                     INRIIARETGQPYEKVVEDTDRNFWIGAQDAVPYGLVSRVIARSDEV"
     gene            2906..>4011
                     /locus_tag="VGM72_00675"
     CDS             2906..>4011
                     /locus_tag="VGM72_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263804.1"
                     /translation="MSLLLTSVAPGALAASFAVPDNGTDNTPKTLTATDAGTVGTNAT
                     LSSSTSTPAVTWTGTTGAGPTLTNSGTIQNIFNTEASSGRAIDMNFTSTSSSLTLTNN
                     VGATITSANDVFRINKSIGAGTVTVNNSGTMISTGSNGDTNGQAIDFNANTTTTGNVI
                     INNFATGIMSSADADGLRPGNNVTINNYGSISGNTTGDTGNDGIDFQDPGKSGTINNF
                     GATSTITGARHAITGKEAVTVYNEGTITGQAGSGLNIDTTTNTTVMSVTNASTGKIIG
                     NAIGGADADGIDIDRLANIDNSGKIQAVGLSSSPNLNEAIAIGGGSITNHVGGTITSD
                     QRAITVDDSNLGNAFAAVTISNEGTISGANGEAI"
BASE COUNT          834 a         1178 c         1189 g          810 t
ORIGIN      
        1 agctccgcac ggtcgacggc ggcaggaatc tgagcggtct ccaatagcac tgtgggcgca
       61 agaaattttt gctccacatg cgatgcagag aggatgttct ggatagcggt gacccgctct
      121 tcaaccagtg gaacgcctcc gggagtgtcg cgcggaacgg cgatttcgat cttcgacatc
      181 ttgctttgca atgctgctcc cgcggcctgc cggacaatct gttccgcttc gcgtgcaagc
      241 tggctcgtac ccggcacgaa gaacacgacg aagaggtttt gtttcggggc agccggcatc
      301 gtgttggggc gaagcaacgt gcaccccgtc aggaagctta acaatgtcac aacaaccaat
      361 gtgcgacgca cgatcgcctc cgattggatc gatcatcggc catgtgaacc ccgaaaggaa
      421 gccccggctt tcttcaacgt gacagaaaca gatacccgct caatggatga gacgcagcga
      481 cgctgtccaa ccctctagcc gctcgcttgc gccgtgctaa gttcggatcg caatcaaacc
      541 gatatcgttt tccattgtct gtgctgcgtg cattcttttt tggcgttctt gcctggggct
      601 tcacttgcgg gatgagcgcc gttgctcaga acgatgtcgc ccctgacctt cgggatcgtc
      661 ccccggtggc ggcccccacg tcgaaaatcg aaacgggcgc cattccgata gtcctgtcgg
      721 aaagccagga ggtcgataat tttgccgccg gcctgatgca gggattgatg gccggatctc
      781 gtgtccgggg tatcgcgctc gtggttgtca aagacgatca tgtgatgttg cagcgaactg
      841 ccggggcaat tgcgccggat atgcgtttta aggggggagg cttgtcggac gtgttcgctg
      901 cggtggcggt catgcagcag gtggaacgcg ggcgattgac ggtgacggcg gatatttcca
      961 gagccctggg tgaatcgaat ccgcatgggg taacgcttga gcaggtgctg acgtttcagg
     1021 gcggcgattc ctcacttcca gtccgcgccg tcgaaaaagc atccggcacc gggtggcctg
     1081 attatttcgc aaaggcaatt gcccagccgc tcggcatgac tgccacggct gttcgtggtg
     1141 cagaactgga aacgaatctc acggacatga gtcattttgc cattgcgctg gtgaatgatg
     1201 gcgtctttca aaatggacgc atcctgatgc cggcctccgt tgaatcgatg caatcgactc
     1261 actacacatc ttatcctttg cttccgggcg cggcttacgg tttcacagag atgcgccgcc
     1321 acgcttggcg cgcgctgcag catgatggcg ccgcgggtga tttcgcgtcg cggctggtga
     1381 tcgtgccgga agcgaaactc ggctatttca tcgtcgtcca aggggccgag ggaccggaat
     1441 tctggcgggc gctcgacaat ggtctgttcg acagattgct gcctgcgcag ggtcatgacg
     1501 tgcgcgctac tccagccggc gcgccggcgc ccacacaagc cgacgccgag gctatggcgg
     1561 gcccctatga atccattcgc accctggcca cggacgcagc gccattgaag ctcggcggcc
     1621 gtttcaccgc gcgtgccggc ggatcggccg aactcatcct gaccggttca ccaagcgcta
     1681 cactgacgcc acgccccggc ggatattggg gcagcgcaga cggcaatctg attgccgtgc
     1741 cggtgatggg aaatttgatg ctcagtaccg gtgcatatgg ccgtcttgcc ctgtacaaac
     1801 gccccgggct ttatcttgcc ctcgcgcttc tggcgttgat tgcggcgggg agtcttttca
     1861 ttttcgaacg gcgcgggaag caaccacggc gtttgcctat gagcccggtg ctgggccttg
     1921 ccggcgcggg ggtgattttc gctctgctat ccgcggttct gtggctgcta tcgccgggcg
     1981 cctgatcgat ggcgcgaaat ttcacgattc tcaaccggac cccgacacgc caggatgcct
     2041 atgccgcttc atgattcggt agggtataag gtccattttt tccaagccct tgccgaactt
     2101 tgatggcatc gccaccttct atagaatgcc gccttccagc cccgttagga tgatcaaaat
     2161 gacaagaatc ctgcctgtat cgctcgatga tgacgacgag aaagagaaaa aagaaaatga
     2221 gggcatgaag cccgagcaac cgatggcgcc ggtgcagatg gcgctgttca aggcgcgcac
     2281 ggtcctgatc ttcggcgaga tcgacatgaa agtcgccgaa cgggtttgcg gccagctcat
     2341 cgcctatgct gccgacggca agggcgatat ccgggtgatc atcaattcgc ccggcggcca
     2401 tgtagaatcg ggcgatacga tccatgacat gatccgcttc tgcggacccc gcgtgaagat
     2461 gatcggcacc ggctgggtgg cgagcgcggg cgcgcatatt ttcttaggcg ccgaaaagga
     2521 aaatcgtttc tgtttgccca atacgcgctt cctgctgcat cagcccttgg gcggggtaac
     2581 cggccaggcg tcggacatca ccatcgaggc ggaggaaatc atcaagatgc gcgagcgcat
     2641 caaccgcatc atcgcgcgcg aaaccggaca gccctatgaa aaggtcgtcg aggacaccga
     2701 ccggaatttc tggatcgggg cgcaggacgc ggtcccctat gggctggttt cgcgcgtcat
     2761 cgcccgtagc gacgaggtgt aacgcacctc gccgttgtca tctcagtttc acaatgcctc
     2821 tctaggctcg cctcacagat aaacgagtgg ggtcagcatg ccggtcaaat ttgttgcgcc
     2881 gcgccttcgg cgcgcgtcgt tgcttgtgtc tttgcttctt acgagcgtag ccccgggtgc
     2941 gctggcggcg tccttcgccg tgccggacaa cggcacggac aatactccaa agacgctcac
     3001 cgccacggat gcgggtactg tcggtacgaa tgcgacgctt tcgtcgagca cgtcgacgcc
     3061 ggccgtcacg tggactggaa ccacgggagc ggggccgacg ttgaccaatt ctggtaccat
     3121 ccagaatatt ttcaacacgg aggcgagttc gggccgagca atcgacatga acttcacctc
     3181 tacaagcagc agtttgacgt tgaccaacaa tgtcggtgca acgattacct ctgccaacga
     3241 cgtattccgg atcaacaaat caatcggcgc cggtacggtg accgtcaaca attctggcac
     3301 gatgatttcg acgggctcga acggcgacac caacgggcag gcgatcgact tcaacgccaa
     3361 cacgaccacc accggcaacg tcatcatcaa caatttcgca accggcatta tgtcctccgc
     3421 cgatgctgat gggttgcggc ccggcaataa cgtcaccatc aataattacg gatcgatcag
     3481 tggaaatacc acgggcgaca ccggcaatga cggcatcgat ttccaggatc cggggaaatc
     3541 cggaacgatc aataatttcg gcgccacaag caccattacg ggggcgcgcc acgccatcac
     3601 cggcaaggaa gcggtcaccg tctacaatga aggcacgatc accggtcagg caggatctgg
     3661 cctgaatatc gataccacca ccaacacgac cgtaatgtcc gtgaccaatg cgagcacggg
     3721 caagatcatc ggtaacgcca tcggcggagc ggatgccgac ggcatcgata tcgatcgtct
     3781 cgcgaatatc gacaattccg gcaagattca ggccgtgggc ctgtcgtcgt cgcccaattt
     3841 gaacgaagcc atcgccatcg gcggtggctc catcaccaat catgttggcg gcacgatcac
     3901 gagcgatcaa cgcgcgatca ccgtagacga ctcaaatctc ggcaatgcgt tcgcggccgt
     3961 cacgatcagc aatgagggca ccataagcgg ggccaacgga gaggcgatca g
//
LOCUS       DASYEG010000012         4935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2323168, whole genome shotgun sequence.
ACCESSION   DASYEG010000012 DASYEG010000000
VERSION     DASYEG010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2323168"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VGM72_00680"
     CDS             complement(<1..261)
                     /locus_tag="VGM72_00680"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044135.1"
                     /note="catalyzes the formation of L-tryptophan from
                     L-serine and 1-(indol-3-yl)glycerol 3-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEY4263805.1"
                     /translation="MTPAPNSFRLGPDAAGHFGAFGGRYVAETLMPLLLKLESAYEAA
                     KNDPEFHAELDGLLKHYVGRPSPLYFAERLTRHLGGAKIYFKR"
     gene            complement(272..943)
                     /locus_tag="VGM72_00685"
     CDS             complement(272..943)
                     /locus_tag="VGM72_00685"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002295.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEY4263806.1"
                     /translation="MSVQVKICGLTSREAVEATLKAGAAFGGLVFFPASPRHLGLDPA
                     RGLAALLRGRARIVTLMVDPADSQVNEVVATIAPDLIQLHGKETPARVAEIAGLAKRP
                     IIKAIPVADSADVARAHAYEDVADFILFDAKADAAATRPGGLGAAFDWQLLAGRSFRR
                     PWGLAGGLTPENVGRAVRIAGPYFVDASSGVEDAPGRKSPEKIANFVVAARNAQYTDQ
                     KANAV"
     gene            complement(989..1690)
                     /gene="pyrF"
                     /locus_tag="VGM72_00690"
     CDS             complement(989..1690)
                     /gene="pyrF"
                     /locus_tag="VGM72_00690"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY4263807.1"
                     /translation="MIPNANPIFVALDTPILDTATALAARVAPYVGGLKLGLEFVSAN
                     GPEGVRAIVKSGAPVFLDVKLHDIPNTVAGAMKALAPLGAAIVNVHASGGAAMMRAAA
                     EAAAIVQPRPKLLAVTVLTSLEAADLAAMGISGTPLEQVVRLARLAKASGIDGVVCSP
                     REIAGVRAACGADFLIVTPGVRPAGGELGDQKRVMTPKEALAAGADILVIGRPITAAS
                     DPAQAARAILESLRT"
     gene            complement(1687..1989)
                     /locus_tag="VGM72_00695"
     CDS             complement(1687..1989)
                     /locus_tag="VGM72_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263808.1"
                     /translation="MKLSTLFVLIPVVLIAAVLAVANREDVVFRLDPFAANDSAVVFV
                     MPLFLLVFLSFLVGVLVGAATIALRRGRNARRKRLAASDVSNVPMTDAVRREEPRA"
     gene            complement(2070..2363)
                     /locus_tag="VGM72_00700"
     CDS             complement(2070..2363)
                     /locus_tag="VGM72_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009811081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEY4263809.1"
                     /translation="MIKSELVARLSERYPHLYHRDVERIVSTVLDEISTALASGDRVE
                     LRGFGAFSVKVRPARQGRNPRTGETVSVEEKRVPFFRTGKELRERLNNGDASE"
     gene            complement(2572..3651)
                     /locus_tag="VGM72_00705"
     CDS             complement(2572..3651)
                     /locus_tag="VGM72_00705"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4263810.1"
                     /translation="MTGFWGKKAAAASALPAVGLMLFLLAPASAAERLAAANPRTAVL
                     KPASSPYIDAHTHIDQNHADDAVMLLLGAMDGLNGAKAFIQTEPYGPGNPGAWDVEKI
                     LPAVKKHADKLAFLGGGGTLNPMILEAYQTGNAGPEVQKKFRTRAEEILREGAVGFGE
                     LSIEHFSLPQSPVKDYEYAPADSPLMLLLADIAAEHDVPIDLHMEALPQDIPAPADLK
                     PPNPPRLHGNIAALERLLAHNPLAKLIWAHAGSDNVGYRTPDLNRRLLQAHPNLYMEI
                     KYDPGFPGKDPPIVDGRLKPEWLKLYSDFPDRFIIGSDQHFDPLATVALARAQQNALL
                     LDQLPADLRKKIATENALRIYGSAH"
     gene            3787..4809
                     /locus_tag="VGM72_00710"
     CDS             3787..4809
                     /locus_tag="VGM72_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HEY4263811.1"
                     /translation="MRSQITQMTIALFATAAFAAAAQAQTGPCVNDLPNPYKQVTGWE
                     HMPRSWAPTNNVFVDAKDNVWVMDRCADKGCLGSEENPIWELSPDGKVMKNFGAGLFA
                     FPHTVKPDADGNIWAIDGDAREGKGNQIFKFSPDGKVLMTLGKKGEGGKGNDVFDRPT
                     GIAFAPNGDIFVSEGHAPTFGNSRITKFDKNGKFIKSFGHLGSGDGELKGPHVLAFDS
                     QGRLFVADRSNSRVAIFDQDGKFLAAWKQFGRPSGIFIDKNDVLYVSDSESQDAPGKD
                     TNNPGCKRGIRVGSAKTGKVDYYIPPPTPKDPKNPPPEGVAADSHGNIYAAAVPERSV
                     YKYVKN"
BASE COUNT         1018 a         1541 c         1487 g          889 t
ORIGIN      
        1 gcgcttgaaa tagatcttgg cgccgcccag atgccgggtc agccgctcgg cgaaatagag
       61 cggactgggc cggccgacat agtgcttcag gagcccgtcg agttcggcgt gaaattccgg
      121 atcgttcttg gccgcttcat acgcgctctc caatttgagc agcaacggca taagggtttc
      181 ggcgacataa cgtccgccga aagcgccgaa atgccctgcc gcatcggggc caagccgaaa
      241 gctgttcggg gccggagtca cagcacgctc ctcataccgc gttcgctttc tgatccgtgt
      301 actgagcgtt gcgcgcggct accacgaaat ttgcgatttt ctcggggctc ttacggccgg
      361 gagcgtcctc gacgcccgat gacgcatcga cgaaataggg gcccgcgatg cgaaccgcgc
      421 gcccgacatt ctccggcgtc aatcctcccg caagtcccca gggccgcctg aaactccgcc
      481 ccgccagcaa ttgccagtcg aaggccgcgc ccagcccacc gggacgggtt gccgccgcat
      541 ccgccttggc gtcgaacaat atgaaatctg cgacgtcctc ataggcatgg gctcgcgcaa
      601 catcggcgga atcggcgacc gggattgcct tgatgatcgg gcgtttggcg aggcccgcga
      661 tttcggccac ccgcgccggt gtctctttgc cgtgcagttg gatgagatcg ggcgcaatcg
      721 tcgcaaccac ttcgttgacc tgggagtcag ccggatcgac catcagcgtc acgatgcggg
      781 cgcgcccgcg caggagcgcc gccagcccgc gcgccgggtc aagaccgaga tggcgggggc
      841 tcgccggaaa gaacaccagc ccaccgaacg cggcacccgc cttcagcgtg gcttcgaccg
      901 cctcccggct ggtcagcccg cagattttca cctgaacgct catgggcggg cttatcgccg
      961 aatttccctc cccccgaaag ggggcggatc acgtcctgag actttcaaga atagcgcggg
     1021 cggcttgggc cggatcggag gcagccgtga tcgggcggcc gatcaccagg atatcggcgc
     1081 ccgccgcgag cgcctctttg ggtgtcatca cccgtttctg atcgccaagt tcgccgcctg
     1141 ccgggcgcac ccctggagtc acgatcagga aatcagcacc acaggccgcg cgcacaccag
     1201 cgatttcccg ggggctgcaa accacaccat cgatgccgga ggctttcgcc aggcgggcca
     1261 atcgcaccac ctgttccagc ggtgtccccg aaatccccat ggctgcaaga tcagcagcct
     1321 ccagactggt caggacggtg accgccagaa gcttgggcct cggttgaacg attgccgccg
     1381 cctctgccgc tgcccgcatc atggccgcac cacctgaggc atgcacattg acgatcgccg
     1441 ctcccaaagg cgcgagcgcc ttcatggcgc cggcgacggt gttaggaata tcgtgcagct
     1501 tcacatcgag aaacaccggc gcaccgcttt taacgatggc acggacacct tcggggccgt
     1561 tggcgctgac gaattccaaa cccagcttca gcccacccac atagggggcc acgcgcgcgg
     1621 ccagcgccgt ggccgtatcg agaatgggcg tgtcgagcgc gacaaaaatc ggattggcgt
     1681 ttgggatcac gctctgggct cctcgcgtct gacggcgtcc gtcatcggca cattggacac
     1741 atccgaagcc gcaagccgct tgcgtcgtgc attgcgcccg cgccgcaagg cgatggtggc
     1801 ggcgccaacc agaacgccca ccagaaagct caagaaaacc aacagaaaaa gcggcatgac
     1861 gaagacgacc gcggaatcat tcgcggcgaa aggatcgagc ctgaacacga catcttctcg
     1921 attcgcgacg gcgagcaccg ccgcgatgag gaccaccggt atcagcacga agagtgtcga
     1981 aagcttcatt gccgttcagt agattgcaga aacgcattgg gcaagccgtc atgagacctg
     2041 cgcgctgtca tcgcaagaac aacgttctgc tattcggacg cgtcaccgtt gttcaatctc
     2101 tcgcggagtt ccttgcccgt ccgaaagaag ggtacacgtt tttcctcgac cgaaacggtc
     2161 tcccccgtgc ggggattgcg cccctgacgt gcgggccgca ccttgacgga aaacgccccg
     2221 aaaccccgca actccacacg gtcaccgctg gcaagcgccg tggaaatttc gtccagcacg
     2281 gtcgaaacga tacgttcaac gtcgcgatga taaagatgcg ggtaacgctc ggagaggcgc
     2341 gctaccaatt ccgatttgat cattaccatt gcccccgcat ggcaagctca accgacgcaa
     2401 caggcctgcc cttataactt gccaaaacgt cgccggaacg tcaactgaaa aagtacacta
     2461 agcccctaag aaataaagcg ttttgacgta cggttgcaac gcttgcggac ttcgttcgcg
     2521 atgaaaggcg ccgcgcgtga gattcgcggg cgcctttctt ttgcattttc gtcaatgcgc
     2581 cgaaccgtag atgcgaagcg cattctccgt cgcgattttt ttgcgtaaat ccgccggaag
     2641 ctgatccagc agcagcgcat tctgctgcgc gcgcgccagc gcaaccgttg cgagcggatc
     2701 gaaatgctgg tcgctgccga tgatgaagcg gtcgggaaaa tcggaataga gtttgagcca
     2761 ttcgggcttg agcctgccgt ccacaatcgg cgggtccttg cccgggaaac cggggtcgta
     2821 cttgatctcc atgtagagat tgggatgggc ctggagcagc cggcgattga gatcgggagt
     2881 ccggtatccg acattgtccg agcccgcatg cgcccagatc agtttggcga gcggattgtg
     2941 cgcgagcagt ctttcgagcg ccgcgatatt gccatgcaat cgcggcggat tcggcggctt
     3001 cagatcggcg ggtgccggga tgtcctgcgg cagcgcctcc atatgcagat cgatgggaac
     3061 atcatgttcg gcggcgatat cggcgagcag cagcatgagc ggcgaatccg ccggcgcata
     3121 ttcgtaatcc ttcaccgggg attgcggcag ggagaaatgt tcgatcgaca attcgccgaa
     3181 accgacggca ccttcgcgaa ggatttcctc ggcgcgggta cggaatttct tctgcacctc
     3241 cggaccggca ttgccggtct gataggcttc caggatcatc gggttgagcg tgccgccacc
     3301 gccgagaaac gccaatttgt ccgcgtgttt tttcacggca ggcagaattt tttccacatc
     3361 ccaggccccc ggattgcccg ggccataggg ctccgtctgg atgaaggctt tcgcaccgtt
     3421 cagaccgtcc atcgcgccta acaggagcat cacggcgtcg tccgcgtgat tctggtcgat
     3481 atgggtatgc gcgtcgatat agggagatga agcgggcttc agcactgcgg tgcgcggatt
     3541 ggcggctgcg agccgctccg ccgcgctcgc gggagcaagc agaaacaaca tcaatcccac
     3601 cgcgggcaac gccgatgcgg cggccgcctt tttcccccaa aatcccgtca tcgctgcatc
     3661 cctcccctga taaaatcccc gtccctcagc ctagcacaaa gcgcgttctt gttacgaatt
     3721 gggctcgtat acgaattgcc ccgcctcatg taggctgcgg acaaacaggc acgggaggaa
     3781 accgccatgc gttctcagat cacgcagatg acgattgcat tgttcgcgac ggctgcgttc
     3841 gcggcggcgg cccaagctca gacagggcca tgcgtcaacg atctgccgaa cccgtacaaa
     3901 caggtcaccg gctgggagca catgccgcgc agttgggctc ccacgaacaa cgtcttcgtc
     3961 gatgcaaagg acaatgtgtg ggtgatggat cgctgcgccg acaagggctg cctgggctcg
     4021 gaagaaaatc cgatctggga attgtcgccc gacggtaagg tgatgaagaa tttcggcgcc
     4081 ggcctgttcg ctttccctca tacggtcaag cccgatgcgg acggcaatat atgggccatc
     4141 gacggagacg ccagagaggg caagggcaat cagatcttca aattctcgcc cgacggaaag
     4201 gttctgatga cgctgggcaa gaagggcgaa ggcggcaagg gcaatgacgt cttcgaccgg
     4261 ccgaccggga tcgccttcgc gccgaacggc gatatcttcg tttccgaagg ccatgcgccg
     4321 accttcggca attcgcgcat cacgaaattc gacaagaacg gaaagttcat caagagcttt
     4381 ggccatctcg gctcgggcga cggcgaactg aagggacctc atgtgctcgc cttcgacagc
     4441 cagggacggc tgttcgtggc cgaccgcagc aacagccgtg tggcgatttt cgatcaggac
     4501 ggaaaattcc tcgccgcgtg gaagcagttc ggacgcccga gcggaatctt catcgacaag
     4561 aacgatgtgc tctacgtttc ggattcggag tcgcaggatg cgcccggcaa ggacaccaac
     4621 aatccgggct gcaagcgcgg catccgcgtc ggcagcgcca agaccggcaa ggtcgactac
     4681 tacattccgc cgccaacgcc caaggacccg aagaacccgc cgccggaagg tgtggcggcc
     4741 gatagccacg gcaatattta cgccgccgcc gtgccggaaa gatcggtcta caaatacgtc
     4801 aagaactgag tttcttccgt caccatggcc gggcttgacc cggccatggt gagaggaaag
     4861 cgttcaaaaa cgaaaacgcc cgcgaggatt tcccgcgggc gtttcgattc gcagagtgtg
     4921 cgttatccgg ccggg
//
LOCUS       DASYEG010000013         5856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_5077819, whole genome shotgun sequence.
ACCESSION   DASYEG010000013 DASYEG010000000
VERSION     DASYEG010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5856
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5077819"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VGM72_00715"
     CDS             complement(<1..410)
                     /locus_tag="VGM72_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEY4263812.1"
                     /translation="MGFSMLSREVREIKTAPAGKDALARLIAGIRGCDICAKDLAHGV
                     RPVLRIGPTARLCIAGQAPGIRVHNTGIPYNDPSGTRLRAWLGIDREVFYDESRIAIV
                     PMGFCFPGYDAAGADKPPRNECVRTWHDRLFAVAP"
     gene            complement(376..1149)
                     /locus_tag="VGM72_00720"
     CDS             complement(376..1149)
                     /locus_tag="VGM72_00720"
                     /inference="COORDINATES: protein motif:HMM:NF023288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramidase domain-containing protein"
                     /protein_id="HEY4263813.1"
                     /translation="MANFAGEGRPLSQNGFDAARTAIGVEDVALWAVLSVETSGCGFL
                     ADRRPKILFERHIFHRLTNGRFDAVAPDISAPSSGGYGQSGAPQYARLESAVRLDEDA
                     ALQSASWGLGQIMGENHKAAGFADVKDLVTAMVESEDGQLRAMANFIEASAMAPALRS
                     RDWAQFARRYNGPNFAQNNYDGQLQNFCSLFTTGKLPDLRVRAAQVYLTYRGFSPHGI
                     DGVAGENTSDAVKAFQSSMGAAPTGTIDDGLLDALARSA"
     gene            complement(1228..2490)
                     /locus_tag="VGM72_00725"
     CDS             complement(1228..2490)
                     /locus_tag="VGM72_00725"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEY4263814.1"
                     /translation="MPWLQLQSAFGLGVLVLLAWVLSEDRRAAVSWRAIAAALGLQIA
                     IALVLLGLPAARAGLYSLNHVVDALSSATQAGSSFVFGYVGGGQPPFDVTKARNLNSL
                     AFQALPLVLVMSALSALFWHWRILPVIVRGFAYVLQKTLLIGGAVGLGSASTIFLGMI
                     EAPLLIRPYLARLTRAELFMLFTVGLSTVAGTVMVLYASVLEPAVPGALGHILVASFI
                     SLPAAILVARLMVPGESTTPAEAHAAIAYRSSMDAIARGTEDGLKLYLGIVAMLLVMV
                     ALVALADIVIGELPPVAGAPLSVERIFGWLFWPLVWLYGVPAGEAADAATLLGTKTIL
                     NEFIAYLKLAALPPGVLSPRSRLIMLYAMCGFANLGSVGIMIAGISGMIPERRDEVVE
                     LALKALVSGTIASGMTGAVVGLLPIFPA"
     gene            complement(2520..2846)
                     /locus_tag="VGM72_00730"
     CDS             complement(2520..2846)
                     /locus_tag="VGM72_00730"
                     /inference="COORDINATES: protein motif:HMM:NF019019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HEY4263815.1"
                     /translation="MPRLKSALYVQALIRRCEVAGAAAYLVRRGAEEAGAVFVKLSRL
                     NGTCVVLSPARRGEERVWTKPLGETSDEARAGEYFARQIRFDPDIWIVEIEDREGRAF
                     VDEPIV"
     gene            complement(2975..4663)
                     /locus_tag="VGM72_00735"
     CDS             complement(2975..4663)
                     /locus_tag="VGM72_00735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4263816.1"
                     /translation="MRAWGRVLAQGSDSHGTSLARWHFIRTQSIKGGLGLVLCGLYAA
                     LVGGPDLAEALVFAVVSAPLGLALAGFAPFALETLEGASLGVVAALLSLLILLTGGLA
                     SPFLVWLVLVPFEGALIGRRGAVIFAGCMAFSGVAGVAALEFSGFLPASRLPESHLLL
                     AGAALLVAIAQASAMAIAAQERRQATDAALEAGEARYRFLAESALDLITLHSLDGRIC
                     FASPASFGLLGYRPDELMGIGPAELSHPDDRHAIDIALDDARAGRSATAELRLKTKKG
                     AYIWSELRCRSVHDEIVAVTRDITRRKTHERALIEARDQAEEASRAKSRFLANMSHEL
                     RTPLNAIIGFSEVMTQELFGPLGNARYADYTRLINESGTHLLELINSILDMSKIEAGR
                     FTVVPQLFDLEDTIAQALRFVSLPAERSGIVLARTIAPEARHVRADKRAVLQILINLI
                     SNGVKYTQAGGRVSVAAELSAGRLMISVSDTGVGIGESDLARLGQPFEQVENEYTRSK
                     EGTGLGLALVRALAHLHGGTMTISSALGQGTCVRIILPDAVEVGEKEDAVQAVA"
     gene            complement(4778..5632)
                     /locus_tag="VGM72_00740"
     CDS             complement(4778..5632)
                     /locus_tag="VGM72_00740"
                     /inference="COORDINATES: protein motif:HMM:NF040428.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6456 domain-containing protein"
                     /protein_id="HEY4263817.1"
                     /translation="MSACRRAEGEIVREGRRVLRKLMAREAVLALLPGGESYALMPGG
                     RNGMKPALCVSAEIVEVFRRRDWVAPSAGEGETLAITDAGLGWVKRAFAGEDAFAAQH
                     RMDSLRAVSTPDGSAQTLTVNDGESPIGWLFHRKGLDGRTLITEWQFRAGERLRADFT
                     LAQLAPRLGVDFSAPVVSGRRGAKSGNLPETVLAAKQRFSRALGAVGPGLSDLLFDVC
                     CHLKGLEAAEKGKGWPRRSARIVLRIALDRLAAHYGMGPVVGTGTRLRAWAAEETEEI
                     VEISGRET"
     gene            complement(5629..>5856)
                     /locus_tag="VGM72_00745"
     CDS             complement(5629..>5856)
                     /locus_tag="VGM72_00745"
                     /inference="COORDINATES: protein motif:HMM:NF019903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY4263818.1"
                     /translation="GRARVVFARQTAMYLMRVIYGLTLTEIAAYFGRDRSTVSYACQR
                     IENLRDDPCFDHQLTQLEGLLRSAAEIGLVL"
BASE COUNT         1174 a         1981 c         1766 g          935 t
ORIGIN      
        1 ggcgctacgg caaaaagacg gtcatgccag gtccgcacgc attcgttacg cggcggtttg
       61 tcggctcccg ccgcatcgta gccgggaaag cagaacccca tgggcacgat ggcgatgcgg
      121 ctctcatcgt agaaaacctc acgatcgatg ccgagccagg cgcgcaggcg ggtccccgac
      181 ggatcattgt aaggaatgcc ggtattgtgg acgcggatgc ccggcgcctg acccgcgatg
      241 caaagccgcg cggttggacc gatgcgcaac accggccgca caccatgggc gagatccttc
      301 gcacagatgt cgcagccgcg gatgccggcg atcagcctcg caagagcgtc tttgccggca
      361 ggcgcggtct tgatctcacg cacttcgcgc gagagcatcg agaagcccat cgtcgatcgt
      421 tcctgtgggt gcggcgccca tcgaagactg gaacgccttg acggcatcgc tggtgttctc
      481 gcccgcgacg ccgtcgatcc cgtggggcga aaatcccctg taggtgagat agacctgcgc
      541 cgcgcgaacg cgcaaatcgg gcaattttcc ggtcgtgaac aggctgcaga agttctggag
      601 ctggccgtca taattgttct gggcgaaatt cggcccgttg tagcggcgcg cgaactgggc
      661 ccagtcccgg ctgcgcaagg ccggcgccat ggcgcttgcc tcgatgaagt tcgccatcgc
      721 ccggagctgc ccatcctcgg actcgaccat ggccgtaacc agatctttca cgtcggcgaa
      781 accggcggct ttgtgattct cgcccatgat ctgtcccaga ccccatgacg cgctctgcag
      841 ggcggcgtcc tcatccaatc gtacagccga ttcgagacgc gcatattgcg gcgcgccgct
      901 ctggccataa ccgccggaag atggcgcgct gatgtcgggc gcgaccgcgt cgaagcggcc
      961 attcgtcaag cgatggaaga tgtgccgctc gaacagaatc ttcggccggc ggtcggcgag
     1021 aaaaccgcac cccgatgttt cgaccgacag gactgcccag agcgccacgt cctcgacgcc
     1081 gatggccgtc ctggcagcat cgaatccgtt ttgcgagagc ggccgcccct cgcccgcgaa
     1141 atttgccata tgatcatcca tcattgtggt ctaccggaac cgcgcgcgat tttagcctat
     1201 cttttgcgtg aacgagaccg gcggcgtcta ggctggaaaa atcggaagca ggcccaccac
     1261 cgcgccggtc attcccgacg cgatcgtgcc cgaaacgagg gccttcagcg ccaactccac
     1321 cacctcgtcg cggcgttccg ggatcatgcc ggaaatcccc gcgatcatga tgccgaccga
     1381 acccagattg gcgaacccgc acatcgcata aagcatgatc agccgcgaac gcgggctgag
     1441 cacgcctggc ggcaaggccg cgagcttcag ataggcgatg aactcgttga ggatcgtctt
     1501 ggtgcccagc agcgtcgcgg catcggccgc ttcccccgcc ggtacgccat agagccacac
     1561 caacggccag aacagccagc cgaagatgcg ctcgacactg agcggcgcgc ccgcgaccgg
     1621 cggcagttcc ccgatgacga tgtcggcgag cgccaccaac gcgaccatga caagtagcat
     1681 cgccacgatg ccgagataaa gcttcaaccc atcctcggtg ccgcgcgcga tggcgtccat
     1741 gctcgaacga taggcgatgg cggcgtgggc ctcggccggc gtggtgcttt cgccgggaac
     1801 catcaaacgc gccaccagga tcgccgccgg caaggaaatg aatgacgcga ccagaatatg
     1861 tccgagcgcg cccggcacgg ccggttcgag aacgctcgcg tagagcacca tcaccgtgcc
     1921 ggcgaccgtg gacagcccga cggtgaaaag catgaacaat tcggcgcgcg tgagacgcgc
     1981 cagatagggc cggatgagaa gcggcgcttc gatcatgccg aggaaaatcg tcgaagccga
     2041 acccagccca accgcgccgc cgattaaaag cgtcttctgc agcacatagg caaagcccct
     2101 cacgatcacc ggcaagatac gccaatgcca aaagagggcc gaaagcgccg acatcaccag
     2161 caccagcggc aaggcctgga atgccaggct gttcagattc cgcgccttcg tcacgtcaaa
     2221 gggcggctga ccgcctccga catagccgaa cacgaaggac gatccggcct gtgtcgccga
     2281 ggacagcgca tcgaccacat gattcagaga atagagtccc gcacgcgccg ccggcagccc
     2341 cagaagcacc agcgcgatag cgatctgaag tcccagcgca gccgcgatgg cgcgccatga
     2401 gacggcggcg cgccggtctt cggagagcac ccaagccagg agcacaagca caccaagccc
     2461 gaacgcgctc tgcaactgaa gccatggcat gaagagaatc gctgagcggc gaacccagtc
     2521 tacacgatcg gttcatccac gaaggcgcgg ccctcgcggt cttcgatctc cacgatccag
     2581 atgtcggggt cgaaacggat ctgcctcgcg aaatattctc ccgcccgcgc ctcgtcgctg
     2641 gtctcgccca ggggtttcgt ccacacccgc tcttcaccgc gccgcgcggg actcaaaacc
     2701 acgcaggtgc cgttcagccg gctcaatttc acgaacaccg cgccggcctc ctccgcgccc
     2761 cggcgcacga gataggcagc cgcgcccgcc acctcgcagc gccggatcaa ggcctggaca
     2821 tagagtgcgc ttttgagccg cggcatggct gggagaccgg ctggagaaat cgagccaccc
     2881 tagagcggtc tcgcttcaag taaaatcgcg atcgtcttcg gccattttat ttgtgccgcg
     2941 caattgcgcg atgaccgggt tgacgccgtg agaatcacgc caccgcctgg acggcgtctt
     3001 ctttctcgcc cacctcgact gcgtcgggca atatgatccg gacgcacgtc ccctgcccca
     3061 gggccgatga gatcgtcatg gtgccgccgt gcaaatgcgc gagcgcgcgc accaacgcca
     3121 gccccagacc cgttccttcc ttcgaacggg tgtattcgtt ttcgacctgt tcgaagggct
     3181 gaccgagccg cgccaaatcc gattcgccga ttcccacacc cgtgtcggaa accgagatca
     3241 tcaaccggcc ggcgctcaat tcggcggcca ccgagacgcg tccgcccgcc tgcgtgtact
     3301 tcacgccgtt ggaaatcaga ttgatcagga tctgcagcac cgcgcgtttg tcggcccgca
     3361 catgacgcgc ctccggcgcg atggttcggg cgagcacgat gccgctgcgt tcggccggca
     3421 gcgagacgaa acgcagggcc tgagcgatgg tgtcttcgag gtcgaaaagc tgcggcacca
     3481 cggtaaaacg gccggcctct atcttggaca tgtccaggat ggaatttatc agttccagaa
     3541 gatgcgtgcc gctttcgttg atcagccgcg tatagtcggc atagcgcgca tttccgaggg
     3601 gaccgaacaa ttcctgcgtc atcacttcgg aaaagccgat gatggcattg agcggcgtgc
     3661 gcaactcgtg actcatattg gcgaggaacc gcgacttcgc gcggcttgcc tcctccgcct
     3721 ggtcgcgcgc ttcgatcaac gcccgctcat gggttttcct gcgcgtgatg tcgcgcgtca
     3781 ccgccacgat ctcatcatgg acggaccggc agcgcagctc gctccagata taggcgccct
     3841 ttttcgtttt gagccgcaat tcggcggtcg ccgagcggcc cgcgcgcgcg tcgtcgaggg
     3901 cgatatcgat ggcatgacga tcgtcgggat gagacagctc ggcgggcccg atgcccatga
     3961 gttcgtccgg cctgtagccg agcaggccga atgacgcggg actggcgaaa catatgcgcc
     4021 catccaggct atgcagcgta atgagatcca gcgcgctttc ggcgagaaaa cgataacgcg
     4081 cctcgcctgc ctccagcgcg gcgtccgtgg cctgccggcg ctcctgcgcg gcgatggcca
     4141 tggccgaggc ctgtgcgatg gccaccagaa gcgccgcacc ggcaagcagc agatgggact
     4201 cgggaagacg cgacgccgga agaaatcccg aaaattccag ggccgcgacg ccggcgacgc
     4261 ccgaaaacgc catgcagccg gcaaatatca ccgcaccgcg gcggccgatc agcgcgcctt
     4321 cgaacggcac cagcacaagc cagaccagaa aaggcgatgc gagcccgccg gtcagcaaaa
     4381 tcaggaggga caagagggcg gcgacaacac cgagggaggc gccttccagg gtttcgagcg
     4441 cgaacggcgc aaagccggcc aatgccagcc ccagaggcgc ggagacgacc gcgaaaacca
     4501 acgcttccgc gagatcgggc ccgcccacca atgccgcgta aaggccgcac aacacgagcc
     4561 ccaggccgcc tttgatgctc tgagttcgga tgaagtgcca tcgcgcaagg gacgttccgt
     4621 gtgaatcgct gccttgcgcc aatacccgtc cccacgcacg cacggttgcc gaatccttac
     4681 cgtggccgat tatcgtttca tcctcgcaaa cgatacttaa cgaatgctga agaccaagcg
     4741 ccacgccttg gcttggcgct gcgaaaatgg cgaagattca ggtttcgcgg cctgaaatct
     4801 ctacgatctc ttccgtctcc tcagccgccc acgcccgcag ccgggttccc gtgccgacaa
     4861 caggtcccat tccgtaatgc gccgcgaggc gatcgagcgc gatccggagc acgatccttg
     4921 cggaacgccg cggccaaccc ttcccttttt cggcggcttc aagacctttg agatgacagc
     4981 acacatcgaa gagcaggtcc gaaagccccg ggcccaccgc gccaagcgcg cggctgaagc
     5041 gctgcttcgc agcaagcacg gtttccggca aattgccgga ttttgcgccg cggcgtccgc
     5101 tcaccaccgg cgccgagaaa tcgacgccaa gccgcggcgc gagctgtgcc aatgtaaaat
     5161 ctgcgcgcaa tctttcgccg gcccggaatt gccattccgt gatcagcgtg cgcccgtcca
     5221 gccccttgcg atgaaacagc caaccgatgg gagactcccc atcattcacc gtgagagtct
     5281 gcgcgctgcc gtccggcgtg gacacggcgc gaagactgtc catccggtgc tgagccgcga
     5341 aggcatcctc gccggcgaag gcgcgcttga cccagccgag tccggcatcg gtgattgcca
     5401 gggtttcacc ctcgcccgcg ctcggggcaa cccaatcccg ccggcggaac acctcgacaa
     5461 tttcggccga aacacacagg gccggtttca tcccgttgcg gccacccggc atcagcgcgt
     5521 agctctcgcc tcccggcaaa agcgcaagca ccgcttcgcg cgccatcagc ttacgcagca
     5581 cgcggcggcc ttctcggacg atttcgcctt cggcccggcg gcacgcgctc atagcaccaa
     5641 tccgatctcg gcggcgctcc gcaacagccc ttcgagttgc gtcaattgat ggtcgaaaca
     5701 gggatcgtca cgcaaattct cgatgcgctg gcacgcatag gagaccgtgc tgcggtcacg
     5761 gccgaaatac gccgcgatct ccgtcagcgt gaggccgtag atcacgcgca tcaggtacat
     5821 tgccgtttgg cgggcaaaga ccacgcgggc ccggcc
//
LOCUS       DASYEG010000014         2280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2323780, whole genome shotgun sequence.
ACCESSION   DASYEG010000014 DASYEG010000000
VERSION     DASYEG010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2323780"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1426)
                     /locus_tag="VGM72_00750"
     CDS             complement(89..1426)
                     /locus_tag="VGM72_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY4263819.1"
                     /translation="MWPFQPALSAFAAGTDTPSDFLECCLACIDAHEADVGAFVHVNK
                     DGARAAAARSTMRWKAGKPLSRIDGMPVGIKDIVETIDMPTEMGSPLYKGWRSGRDSA
                     SVAALREAGAVIVGKTVTTEFAATSPGRTRNPWDTARTPGGSSSGSAAGVAAGMISAG
                     LGTQVVGSIVRPASFCGCVGFKPSAGAINRGGSHDGLSQSAHGVLAASLEDAWNFCQA
                     IASRAGGDPGFPGLAGPEDMPRATRPRTLAVLETSGWALASDAAKAALEETVVRLHAA
                     HIPIVRQRDNPLVGEVEQQLSKARAITDAVNAWESRWPLNTYRARDAGALSKFMLERL
                     AHAEAMTLEDYRRALGERVRMRAAYTKLRPIADAAITLSAIGAAPRGLVSTGNPVFAV
                     AASALGVPAVSLPVLADEGLPLGLQVMGFEQEDAALVGIAAWLRDFVSGSDIA"
     gene            complement(1447..1833)
                     /locus_tag="VGM72_00755"
     CDS             complement(1447..1833)
                     /locus_tag="VGM72_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263820.1"
                     /translation="MPMNKPHLEFTMLDLDSGWATPRGYPSGIKQKILASDIDEARKM
                     GSRTRLLRFDPGSYTTAPFVHDHWEEVYLLSGDLIVGNDAKGEGGEAFLSPTYACRPP
                     GIHHGPFKSETGCMLYEIHYYDESRN"
     gene            complement(1865..>2280)
                     /locus_tag="VGM72_00760"
     CDS             complement(1865..>2280)
                     /locus_tag="VGM72_00760"
                     /inference="COORDINATES: protein motif:HMM:NF022463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2848 family protein"
                     /protein_id="HEY4263821.1"
                     /translation="EGVLWIGTGSDHTDREVEAYGVSVSKQMCEKPVAPQFWRFSDVA
                     AHWDRLILRSHAIIGGRRVLYQEGSVAAMRHPEDLIGRFAKNGLAEGILMFCGTLAVH
                     GGVRAAERFEFELEDPVAARKIQHGYDVVSLPNLG"
BASE COUNT          441 a          748 c          709 g          382 t
ORIGIN      
        1 tcgagaccct catcgcggaa gaaccccagc tcgacggccg ctgcggcagg aaaataggaa
       61 ttggagatga tgtcggggac cgcgagcttc atgcaatgtc cgatcctgaa acaaaatccc
      121 ggagccaggc cgcgatgccg acgagagccg cgtcttcctg ttcgaagccc atgacctgaa
      181 gtccgagcgg caaaccttca tcggcgagca cgggaagaga gaccgcgggg acaccgagcg
      241 cggaggccgc gaccgcgaag acgggattgc cggtggagac gagaccgcgc ggcgcggcgc
      301 cgatggccga aagcgttatc gccgcgtcgg caatcggccg aagcttggtg taggcggcgc
      361 gcatacgcac gcgttcgccg agcgcgcggc gatagtcctc aagcgtcatg gcttcggcat
      421 gagccagccg ttccagcatg aatttgctca gcgcccccgc gtcacgggcg cgataggtgt
      481 tgagcggcca gcgcgattcc caagcgttca ccgcatcggt gatcgcgcgc gcctttgaca
      541 attgctgttc gacctcgccg accagcggat tgtcgcgctg cctgacgatg gggatatggg
      601 ccgcatgcag acgcacaacc gtttcctcaa gcgcggcttt cgcggcgtcc gacgcgagcg
      661 cccacccgct cgtctccagc accgcgagcg tgcgcgggcg cgtcgcgcgc ggcatgtcct
      721 cgggaccggc cagtcccgga aaaccaggat cgcctccggc gcggctcgcg atcgcctgac
      781 aaaaattcca cgcatcctcc aggctcgcgg ccagcacgcc gtgcgcgctt tgcgagagac
      841 cgtcatggct gccgccgcga ttgatcgcgc cggcgctggg tttgaacccg acgcagccac
      901 agaagcttgc cgggcggacg atggaaccca caacctgagt tcccaaaccc gccgagatca
      961 tacccgccgc cacgcctgcc gccgaaccgc tgctggagcc gccgggggtg cgggccgtat
     1021 cccagggatt gcgcgtgcgt ccaggcgatg tggcggcaaa ttccgtcgtc accgtcttgc
     1081 cgacgatcac cgcgccggct tcgcgcagcg cggcaacgct cgccgaatcg cggcctgagc
     1141 gccagccctt gtaaagcggc gatcccattt cggtcggcat gtcgatagtt tcgacaatgt
     1201 ccttgattcc gaccggcatg ccgtcgatcc gcgaaagcgg cttgcccgct ttccagcgca
     1261 tcgtggatcg cgccgcggcg gcccgcgccc catccttgtt cacatgcacg aaagcgccca
     1321 cgtccgcttc atgcgcgtcg atgcaggcca ggcagcattc caaaaaatcg ctgggcgtat
     1381 cggtgccggc ggcaaaggcg gaaagagcag gctggaatgg ccacatcgaa aaaatgtccg
     1441 ctgccgctaa ttgcggctct cgtcgtaata gtggatttcg tagagcatgc agccggtttc
     1501 cgatttgaac gggccatgat gaataccggg cgggcggcag gcataggtcg ggctcaagaa
     1561 cgcctcgccg ccttcgccct ttgcatcatt gccgacgatc agatcgccgg aaagaagata
     1621 aacctcctcc caatgatcgt gcacgaaagg cgcggtggtg taagaacccg gatcgaagcg
     1681 caacagccgc gtgcggcttc ccatcttccg ggcttcgtcg atatcgctcg ccaggatctt
     1741 ctgcttgatg cccgagggat aacctctggg cgtcgcccag ccgctatcca ggtcgagcat
     1801 ggtgaattcc agatgcggct tgttcatcgg catggcggat tccttcatcc ctaacgcaaa
     1861 tggcctaacc caaattgggc aagctcacga cgtcatagcc gtgctgaatt ttgcgcgcgg
     1921 cgacagggtc ttcaagttca aattcgaaac gttccgcggc gcgcacgccg ccatgcacgg
     1981 cgagcgtgcc gcagaacatc aagataccct cggcaagacc gtttttcgcg aagcgcccga
     2041 tcaaatcctc cggatggcgc atcgccgcga ccgagccctc ctgatagagg acgcggcggc
     2101 cgccgatgat ggcgtgagag cgcaggatca gccgatccca atgggcagcg acatccgaaa
     2161 atcgccagaa ttggggcgcg accggcttct cgcacatctg cttggagacc gaaacgccat
     2221 aggcttccac ctcgcggtcg gtgtgatccg agccggtgcc gatccagagc acgccttcag
//
LOCUS       DASYEG010000015         5171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4788609, whole genome shotgun sequence.
ACCESSION   DASYEG010000015 DASYEG010000000
VERSION     DASYEG010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4788609"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VGM72_00765"
     CDS             <1..990
                     /locus_tag="VGM72_00765"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEY4263822.1"
                     /translation="QDSSRGTFSQRHAALVDQETEERYIPLNHISEGQPEFEVVDSLL
                     SEEAVVGFEYGYSLAEPKTLVLWEAQFGDFANGAQVVIDQFIASGEMKWLRMSGLVML
                     LPHGFEGQGPEHSSARLERYLQLCADDNMQVACCTTPANYFHVLRRQLHRNFRKPLIL
                     MTPKSMLRNKRAVSKLSEFGPGSSFHRVLWDDAESSPGSTVALVPNEEIARVVLCSGK
                     VYYDLLEEREKRGETRIQLLRVEQLYPFPGLALTDELTRFPKAEIIWCQEEPKNQGAW
                     TSVAHSIESVMEQLGRKGPLRYTGRKASASPAAGQMSTHLTELKNFLNEALTL"
     gene            1054..2271
                     /gene="odhB"
                     /locus_tag="VGM72_00770"
     CDS             1054..2271
                     /gene="odhB"
                     /locus_tag="VGM72_00770"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005400412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEY4263823.1"
                     /translation="MSIEIKVPAMGESVTEATIARWLKKEGDSVNRDEPLVELETDKV
                     SVEVPSPAAGVLEHISVQQGGTVGVGTVLGAVAEGAAAKAPSQQSKTQSAKPVKAEAP
                     KAEPKPAPASAPVPPPPPSVRRIAEEAKLDVSAIAGSGKRGQLTKADALAALEAAKPP
                     AAAAPAQPRAGRAEREERVAMSRLRKTIALRLKESQEIAAQLTTFNEVDMSKVMALRS
                     TYKDGFEKKHGVKLGFMGFFVKACIAALKEIPAVNAEIDGDDIVYKNYYDIGVAVSTD
                     RGLVVPVVRDAESLSLAEAEKAIADFATRARDNKLKLEELLGGTFTITNGGVFGSLMS
                     TPILNLPQSGILGMHKIQNRPVAIGDKIEIRPMMYLALSYDHRLVDGREAVTFLVRVK
                     ENLEDPQRLLLDL"
     gene            2275..3675
                     /gene="lpdA"
                     /locus_tag="VGM72_00775"
     CDS             2275..3675
                     /gene="lpdA"
                     /locus_tag="VGM72_00775"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY4263824.1"
                     /translation="MAENFDVVVIGGGPGGYNCAIRCGQLGLTVACIDKRGPFGGTCL
                     NIGCIPSKALLHASDLLEVARYDFPRFGITATPSFDLKAMMAQKDKVVGELTKGVEFL
                     FKKNKVTPIVGEGKIAGPGRVSVKTKDGAREIACKHIVIATGSDVAPLAGVSIDEKTI
                     VSSTGALSLPAVPKRMLVVGAGVIGLELGSVWRRLGSEVQIVEFLDRIVPGVDAEVAK
                     QFQRLLERQGMKFKLASKVVGLEKKADGYTIAVEPAQGGARETLEADVIMVCVGRRPF
                     TENLGLDEAGVKRDARGRVMTDAHLQTNVPGIWAIGDVREGAMLAHKAEDEAVACAEN
                     IAGRAGHVNYDAIPSVIYTYPEVATVGKTEEELKAAGIAYKVGKFPFTANPRAKTILS
                     TEGFVKVIADAATDRVLGVHMIGPDVGNMIGEASLAIEFSASAEDIARTSHAHPTLTE
                     AIRQAAMAVDGWAMQM"
     gene            complement(3760..4239)
                     /locus_tag="VGM72_00780"
     CDS             complement(3760..4239)
                     /locus_tag="VGM72_00780"
                     /inference="COORDINATES: protein motif:HMM:NF017950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF992 domain-containing protein"
                     /protein_id="HEY4263825.1"
                     /translation="MSKKLGLTIGVAILAAASWLAPAQAAGVKIGVLTCNVDSGWGYI
                     LGSSKDVRCNYVPNKGFGEHYVGSVSKVGVDIGYTKGGVLIWDVIAPSSDMKPGALEG
                     NYGGVSASATVGIGAGANVLLGGFDKSVALQPISIEGNTGLNVAAGIGALDLKFEKG"
     gene            complement(4401..4829)
                     /locus_tag="VGM72_00785"
     CDS             complement(4401..4829)
                     /locus_tag="VGM72_00785"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4263826.1"
                     /translation="MTQLAKDARCTTITTLRYHDANAAIEFLCDAFGFSRHLVVPGPG
                     NTVAHAQLSFGNGMIMLGSHPHEGEFGKWIQPPQAPAMVNTHSVYVVVADVDAHHARA
                     QACGANIIRPLTDQDYGGRDYSARDPEGYVWSFGTYDPWA"
     gene            complement(4826..>5171)
                     /locus_tag="VGM72_00790"
     CDS             complement(4826..>5171)
                     /locus_tag="VGM72_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263827.1"
                     /translation="VGINLAVQDAVAAANILAVPLRSGTPPLSVLRMVQKRRMLPTRL
                     TQGMQRLIQDRVMTRVLGLTHEPKPPLFVSLLDRFAFLRRIPARLVGIGFRPEHVRSP
                     ECKPDRYKGEEP"
BASE COUNT         1088 a         1551 c         1578 g          954 t
ORIGIN      
        1 caggattcct cgcgcggaac attctcgcaa cgccacgccg cgttggtcga tcaggaaacc
       61 gaagaacgct acattccgtt gaaccacatt tcagaaggcc agccggaatt cgaagtcgtc
      121 gattcgcttc tctccgaaga ggctgttgtc ggtttcgaat atggctacag ccttgccgag
      181 cccaagaccc tggtgttgtg ggaagcgcaa ttcggcgatt tcgcgaatgg cgcgcaggtg
      241 gtgatcgatc agttcatcgc ctccggtgaa atgaaatggc tgcgcatgtc gggccttgtg
      301 atgctcctgc cgcatggatt cgaaggccag ggaccggaac attcttccgc ccgtctcgag
      361 cgctatctac agctttgcgc cgacgacaac atgcaggtcg cctgctgcac gacgccggcg
      421 aattatttcc acgtcctgcg gcggcaattg catcgcaatt tccgcaaacc cctgatcctg
      481 atgacgccga agtcgatgct gcgcaacaaa cgcgcggtct ccaaactttc ggagttcggg
      541 ccgggatcgt ccttccatcg cgtgctgtgg gacgatgcgg aatcgtcgcc gggttcgacc
      601 gtggctctcg tgcccaatga ggagattgcc cgtgtcgtgc tgtgcagcgg caaggtctat
      661 tacgaccttc tggaggagcg cgaaaaacgc ggcgagaccc gcatccagct tctgcgcgtc
      721 gaacagcttt atccgtttcc ggggcttgcc ttgaccgacg agttgacgcg ctttcccaag
      781 gccgagatca tctggtgcca ggaagagccc aaaaatcagg gcgcctggac atcggttgcg
      841 cactccatcg aaagcgtcat ggagcaattg ggccgcaagg gtcctttgcg ctatacaggc
      901 cgcaaggcat cggcttcgcc ggcggcagga caaatgagca cgcatctgac cgagctcaag
      961 aatttcctca acgaagcgct gacactgtaa tttggcttga aagccttcca tcttcggcac
     1021 acgaaacatg gagcggcgac agaggggcgc agaatgagca tcgagatcaa agtgccggca
     1081 atgggcgaat ccgtcaccga agccaccatt gcccgctggt tgaagaagga aggcgattcc
     1141 gtcaaccgcg acgagccgct ggtcgaactt gagaccgaca aggtaagcgt tgaggttccc
     1201 tcccccgccg ccggcgtgct cgaacatatc agcgtgcagc agggcggtac ggtgggcgta
     1261 ggcaccgtgc tgggcgcggt tgccgaaggg gccgcagcga aagcgccatc ccaacagtcc
     1321 aagacccaaa gcgccaaacc ggttaaagcc gaggccccga aagccgaacc caagcccgct
     1381 ccggcttcag cgccggttcc tcccccgccg ccctcggtgc ggcgtatcgc ggaggaagcc
     1441 aagctcgatg tctccgccat tgccggttcc ggcaaacgcg gccagctcac caaggccgat
     1501 gcgctcgcag cgctcgaagc agcaaagccg ccggcggccg ctgccccggc acaaccgcgc
     1561 gcagggcgcg ccgagcgcga agagcgcgtg gcgatgtcgc gtctgcgcaa gaccatcgcg
     1621 ttgcgtctga aggaaagcca ggagatcgcg gctcagctca ccaccttcaa cgaagtcgat
     1681 atgtccaagg tgatggcgct gcgcagcacc tataaggacg gtttcgaaaa gaagcacggc
     1741 gtcaagctcg gcttcatggg ctttttcgtg aaggcctgca tcgcggcgct caaggaaatc
     1801 ccggcggtca atgcggagat cgacggcgac gacatcgtct ataagaatta ttacgacatc
     1861 ggcgtcgcgg tctcgacaga tcgcggactt gtggtgcctg tggtgcgcga tgcggaatcg
     1921 ctgtcgcttg ccgaggccga aaaggccatc gcggatttcg ccacgcgcgc tcgcgacaac
     1981 aaactgaagc tggaagaact tttgggcggg accttcacca tcaccaatgg cggggtcttc
     2041 ggctcgctga tgtccacgcc cattctcaat ctcccgcaat cgggcattct gggcatgcac
     2101 aagatccaga accggcccgt cgccattggc gacaagatcg aaatccgtcc aatgatgtat
     2161 ctcgcactgt cctacgatca ccggctggtg gacggccgcg aggcggtcac tttcctcgtg
     2221 cgggtgaaag aaaatctgga agacccgcag cgtctgttgc tggaccttta gcccatggcc
     2281 gaaaatttcg acgttgtcgt catcgggggc ggaccgggcg gctacaattg cgcgattcgg
     2341 tgcggccaac ttggcctgac ggtcgcttgc atcgacaagc gcgggccgtt tggcggaacc
     2401 tgcctgaaca tcggctgcat tccttccaaa gcgctgctgc atgcgagcga tctcttggag
     2461 gtcgcgcgct atgattttcc ccgcttcgga attaccgcga cgccaagctt cgacctgaag
     2521 gcgatgatgg cgcaaaagga caaggtcgtc ggcgaattga ccaagggcgt ggagttcctg
     2581 ttcaagaaga acaaggtcac tccgattgtg ggcgaaggaa agattgccgg cccgggccgc
     2641 gtcagcgtga aaaccaagga cggcgcgcgt gaaatcgcgt gcaagcacat tgtcatcgcg
     2701 accgggtcgg atgtggcgcc gctcgcgggc gtcagcatcg acgaaaagac catcgtatcg
     2761 tccaccggcg cattgtcctt gcccgcggtt ccgaagcgga tgctggtggt cggcgcgggc
     2821 gtgattgggc tcgaactcgg ttcagtctgg cgccggctcg ggtccgaggt gcagatcgtc
     2881 gaattcctcg accgcatcgt gcccggcgtg gacgctgaag tcgccaagca attccagcgt
     2941 ctgctggagc gccaggggat gaaattcaag ctcgccagta aagtcgtggg cctagagaag
     3001 aaggccgatg gctacaccat tgcggtcgaa cccgcgcaag gaggcgcgcg cgaaaccctc
     3061 gaggccgatg tgatcatggt gtgcgtcgga cggcggccct tcaccgaaaa tctcggacta
     3121 gacgaagcgg gcgtcaagcg cgatgcccgt gggcgtgtca tgaccgacgc gcatttgcag
     3181 accaatgtac cgggcatctg ggcgatcggc gacgtgcgcg agggtgcgat gctggcgcac
     3241 aaggccgaag acgaagcggt cgcgtgtgcg gagaacatcg caggacgcgc aggccatgtg
     3301 aattacgatg cgatcccgtc ggtcatctac acctatcccg aggtcgccac cgtcggcaaa
     3361 accgaagaag aattgaaggc ggcgggcatc gcctacaagg tcggtaaatt ccccttcacc
     3421 gccaacccgc gcgccaagac cattctatcc accgagggct ttgtgaaggt catcgccgac
     3481 gcggcgacgg accgtgtgct gggcgtgcat atgatcggtc ccgatgtcgg caatatgatc
     3541 ggggaagcgt cgctcgccat cgaattctcc gcctcggcgg aagatatcgc gcgcacctcc
     3601 catgcccatc cgacgctgac cgaggcgatt cgccaggcgg ccatggccgt cgatggctgg
     3661 gcgatgcaga tgtaagcctg gcgaacgcga agagcgcccg cggtttcccg ccaggcgctc
     3721 ttgcgtgaga aaagcgattt caatcgctag acgcgaaaat cagcccttct cgaacttcag
     3781 atcgagcgca ccgatgccgg ctgcgacgtt caaaccggtg ttgccttcga tgctgatcgg
     3841 ctgcagcgca accgatttgt caaagccgcc gagcaggaca tttgcgcctg cgccgatgcc
     3901 aactgtggcg cttgccgaca cgccgccgta attgccttcc aaagcacccg gcttcatatc
     3961 ggacgaaggc gcaatcacgt cccagatgag aacgccgccc ttggtgtagc cgatatcgac
     4021 accgactttg gaaacgctgc cgacataatg ttcgccgaag cctttattcg gcacgtaatt
     4081 gcagcgaaca tccttggacg atccgaggat gtaaccccag ccgctgtcca cattgcaggt
     4141 gagaacgcca attttcaccc ccgctgcctg tgcgggcgcg agccaggacg ccgccgccaa
     4201 aatggccacg ccaatcgtca aacccagttt cttcgacata aatgcctcca tattcccaac
     4261 gtcacaggga tcgccggaaa atccaacccg aacacgaatg cgtgcccggc tattgcaaag
     4321 ggaaacgtgc gcaatcggta caggttccgg cggtacaggt tccggctgca taggttccgg
     4381 cgcggaagat tacgccaggg tcatgcccac ggatcgtaag tgccgaaaga ccagacatat
     4441 ccttccggat cgcgcgccga ataatcgcgg ccgccgtaat cttgatccgt aagcggacga
     4501 ataatattcg ctccgcaagc ttgcgcgcgg gcatgatgcg catcgacgtc cgcgacaaca
     4561 acataaacgc tgtgtgtgtt aaccatagcg ggtgcttggg gtggctggat ccattttccg
     4621 aattcacctt catgaggatg tgaacccagc atgatcatgc cgttaccgaa actcagctgg
     4681 gcatgcgcga ccgtgtttcc ggggccgggc accacgagat gtcttgaaaa tccgaaggca
     4741 tcgcagagaa attcgatggc cgcgttcgca tcgtggtagc gcagcgtggt gatcgtcgtg
     4801 caacgcgcat ccttcgcaag ctgggtcatg gttcttctcc tttgtagcga tccggcttgc
     4861 attcgggcga gcgcacgtgc tcgggacgaa accctatgcc gacgagacgc gccggaattc
     4921 gtctgagaaa cgcgaagcgg tcgagcagcg atacgaacag aggcggcttg ggttcgtgcg
     4981 tcaaaccgag aacgcgcgtc atcacgcgat cctggatgag cctttgcatg ccttgcgtca
     5041 aacgcgtggg caacatgcgg cgcttctgga ccattcgtag aacgctgagc ggcggtgtgc
     5101 cgcttcgtaa cggcaccgcc aggatattgg cggcggcgac ggcatcctgc acggccagat
     5161 tgataccgac g
//
LOCUS       DASYEG010000016         3854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979 k141_5745,
            whole genome shotgun sequence.
ACCESSION   DASYEG010000016 DASYEG010000000
VERSION     DASYEG010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5745"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="VGM72_00795"
     CDS             <1..1041
                     /locus_tag="VGM72_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY4263828.1"
                     /translation="EIDDESALIKVEIAGICGSDVKNYGKPIQNVIMGHENVGVIAKA
                     GRKFQARKNVKEGDRVLLEHYLPDMTCEWCHQGEYRHCEATDWHTNPNAIRYGYTSAD
                     TAPHLWGGFAHYLYAPWNAIVHKVPDNVTAELAGIATPLANGIQWALYEAGVGYGSVV
                     LIQGPGQQGLAQIVACKQAGADCIIVTGTTKDKKRLELAKLLGADYTIDVQTEDPAAR
                     IRQIVPKGVDFSIDCTAGAGTAAMLLGITALKRKAGILVIQGNEMPTFPDFPIYRVTQ
                     KYAQLKSARGHSYKACELALRQLASHRFPLEKAITHTFGLSQVDEAIKAVGGKGPADV
                     IHVSVDPWKDAA"
     gene            complement(1102..2268)
                     /locus_tag="VGM72_00800"
     CDS             complement(1102..2268)
                     /locus_tag="VGM72_00800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4263829.1"
                     /translation="MSANPLRSCPLCLSSQSEEVQTQILSLAGLGTVELGFGYCRACG
                     HIHQVRPAPAHILSENYAAYSNYTCFDPAAARAAPPAASTRRLVTLAEARAPRTGKIY
                     EVGCATGFHLAHFRHAGWEVAGCDPSPKAKAQANDIFGIAVDCGMEEDILPRQSDLAV
                     VLFSHVLEHLDDPLAALKRARHALRDDGVVLLEVPCAISAHLMPPGWFTFEHLHYFSE
                     NSLLRMLSEAGLAPVEMRIAFKAEIYPVIAVIAGKSETFSPPAKSELHVAQARQFLAE
                     LVARDDALWNATSARLQAGLGRVYVWGAGVHTAQLFDRTPLLQNANVVGIIDRDNQKW
                     GRSQANREIIGPDDFFARYDDEPVIIYSFASEAQIAQSLMDRDIARNRIVRLYG"
     gene            2391..3497
                     /locus_tag="VGM72_00805"
     CDS             2391..3497
                     /locus_tag="VGM72_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY4263830.1"
                     /translation="MSAKTTLAAVMTSPRQTELRELPLPEIPTDAGLLRVMATGICGS
                     DWGKYLSDKFSPCILGHEIVGTIEKLGNAARDRWGVKEGDYVALEEYLPCGHCEYCRT
                     GEYRSCLETDHFNPKGVRFGSTPLSVAPSLYGGYSQYLYMHPRTVVHRLPPNIPPHIA
                     AMALPLGNGFQWAYMDAGIGPGQTIVVMGPGQQGLGCVVAANVAGADNIVVVGLARDA
                     NRFETARKLGATHTIAVDEEDVRTRIQDITGGLMADIVIDVSSAGPEIVNGGLSLVKK
                     RGAMLCTAWKKTAVPLDLDRLIRFQANLRGVRGHSYDAVELAIGTMQSGKYPLDLIST
                     HVVGLKDVDRALRIVGGETNEPAIHITVEPWKMS"
     gene            3510..>3854
                     /locus_tag="VGM72_00810"
     CDS             3510..>3854
                     /locus_tag="VGM72_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY4263831.1"
                     /translation="MLTVEENERATRVGPGTPAGKLFRRYWWPLCLSTELPEKDGAPL
                     RIRLLGEDLTAFRDTEGKIGLIDAYCPHRRAPLFFARNEECGLRCVYHGWKFDRNGDC
                     VDMPSEPAGTTLQ"
BASE COUNT          785 a         1196 c         1198 g          675 t
ORIGIN      
        1 gaaatcgacg atgaatcggc gctgatcaag gtcgagatcg cgggcatctg cggcagcgac
       61 gtcaagaatt acggcaagcc gatccagaac gtgatcatgg gtcacgaaaa tgtgggcgtc
      121 atcgccaaag cgggacgcaa attccaggcc cggaagaacg tcaaggaagg cgaccgggtg
      181 ctgctcgagc actatctgcc ggacatgacc tgcgaatggt gccaccaggg cgaataccgc
      241 cattgcgaag ccaccgattg gcacaccaac cccaacgcca tccgctacgg ctacacctcg
      301 gccgataccg caccccatct gtggggcggc ttcgcgcatt acctctatgc cccctggaac
      361 gcgatcgtgc acaaggtgcc cgacaacgtg accgccgagc ttgcgggcat cgcgaccccg
      421 ctcgccaacg gcatccaatg ggcgctgtac gaagcgggcg tcggctatgg ctccgtggtg
      481 ctgatccagg gccccggcca gcagggcctg gcccagatcg tcgcctgcaa acaggccggt
      541 gccgactgca tcatcgttac cggcacgacc aaggacaaga agcgcctgga actcgccaag
      601 ctgctcgggg ccgattacac gatcgatgtg cagaccgaag atccggccgc gcgcatccgg
      661 cagatcgtgc ccaagggcgt ggatttctcc atcgactgca cggccggcgc cggcaccgcc
      721 gccatgctgc tcggcatcac cgcgctcaag cgcaaagccg gcatcctggt catccagggc
      781 aatgagatgc cgaccttccc ggatttcccg atctaccggg tgacgcagaa atacgcgcag
      841 cttaagagcg cgcgcggcca cagctacaag gcctgcgaat tggccctgag acagctcgcc
      901 tcgcaccgct tcccgctgga aaaagccatc acccacacct tcgggctgag ccaagtggat
      961 gaagccatca aggcggtagg cggcaagggt ccggcagacg tgatccacgt ctcggtcgat
     1021 ccctggaaag acgcggcgta ggcagcaagc gtttgcagtg acgggcgctc ggccgacggg
     1081 cgcccgtttg cgttttgagc cttagccgta caaccggacg atacgattgc gcgcgatatc
     1141 gcggtccatc aggctttgcg cgatctgcgc ctcggaggcg aaagagtata tgattacggg
     1201 ctcatcgtca tagcgggcaa aaaaatcatc cgggccgatg atctcgcgat ttgcctgact
     1261 gcggccccat ttctgattgt cgcgatcgat aatcccaacg acatttgcgt tttgcagcag
     1321 cggcgtgcga tcgaacaatt gggcggtgtg gacgcccgcg ccccagacat aaacgcggcc
     1381 caggccggcc tgaagacgcg ccgaggtggc attccacaag gcgtcatcgc gcgccaccag
     1441 ttcggcgagg aattggcgcg cttgcgcaac atgaagctcg gatttggcgg ggggcgaaaa
     1501 tgtctcgctc ttgccggcga tgaccgcaat caccggatag atttcggctt tgaaggcgat
     1561 ccgcatttcg acaggtgcga gacccgcctc gctcaacatg cgcagcaaac tgttctcgga
     1621 gaaataatgc agatgctcga aagtgaacca gcccggaggc atcagatggg ctgagatcgc
     1681 gcacggcact tcgagcagga cgacaccatc gtcccgcaag gcatggcgcg cccgcttcaa
     1741 cgccgccagc ggatcgtcga gatgttcgag gacatgggag aagagcacga ccgcgaggtc
     1801 cgattgccgc ggcaggatat cctcctccat gccgcaatcg acggcgatgc cgaaaatgtc
     1861 gttcgcttgc gccttcgctt ttggcgaagg atcgcagccc gccacctccc agccggcgtg
     1921 acggaaatgc gccaggtgaa atcccgtggc acaaccgacc tcatagattt tcccggtccg
     1981 cggtgcgcgt gcttcggcca atgtcacgag acgccgcgtc gaggccgctg gcggcgcggc
     2041 gcgtgccgcc gccggatcga agcaggtata gttggaatag gccgcgtaat tctcgctgag
     2101 gatgtgcgcg ggcgcggggc gaacctgatg gatgtggccg caggcgcggc aatagccaaa
     2161 gcccagctcc accgtgccga ggccggccag agacaagatc tgtgtctgaa cctcttcgct
     2221 ttgcgaagac aggcaaagcg gacaggacct gagcggattt gcggacatgg ggcgaatttc
     2281 tccgccccta tcctcgcaaa acaacattaa cgggttgttg cttggaacaa ccaaaatgcg
     2341 tttgggcgcc cgcgcttgtc agggggactt agcgctctta tacaaatttt atgagcgcca
     2401 agaccaccct cgccgccgtg atgacgagcc cgcgccagac cgagcttcgc gagctgccat
     2461 tgccggagat tcccaccgat gcggggctct tgcgcgtcat ggcgacgggg atttgcggca
     2521 gcgactgggg caaatacctg tccgacaagt tcagcccctg catcctgggc cacgagatcg
     2581 tgggaaccat cgaaaagctt gggaacgcgg cgcgcgaccg ctggggggtc aaggaaggcg
     2641 actatgtcgc gctggaagaa tatctgccct gcggacactg cgaatattgc cggaccggtg
     2701 aataccgctc gtgcctggag accgaccatt tcaacccgaa gggcgtgcgc ttcggttcga
     2761 cgcccttgag cgtcgcgcct tcgctctatg gcggctacag ccaatatctc tacatgcatc
     2821 cccgcacggt ggtacatcgt ctgcccccaa atatcccgcc ccatatcgcg gccatggcgc
     2881 tgcccttggg caatggtttt caatgggcct atatggatgc gggcatcggg ccgggccaga
     2941 ccatcgtggt gatgggaccg ggtcagcagg gtttggggtg cgtggtcgcg gccaatgtcg
     3001 cgggcgcgga caatatcgtg gtggtggggc tggcccgtga cgccaatcgt ttcgagaccg
     3061 ccaggaagct tggcgcgacc cataccatcg ccgtcgatga agaggatgtg cgaacgcgca
     3121 tccaggacat caccggcggc ctgatggccg acatcgtgat cgatgtgtcc agtgccgggc
     3181 ctgagatcgt caatggcggg ctgtcgctcg tgaagaaacg cggcgccatg ctctgcaccg
     3241 cctggaagaa gaccgccgtg ccgctcgatc tcgaccgctt gatccgcttc caggcgaatt
     3301 tgcgcggcgt gcgcggccat tcctatgacg cggtggaact tgccatcggc accatgcaat
     3361 ccgggaagta cccgctcgat ttgatttcaa cgcatgtggt cggcctgaag gacgtcgacc
     3421 gcgcgctcag aatcgtcggc ggcgaaacca acgaacccgc catccatatc acagtcgaac
     3481 cttggaaaat gagttgagga gtaatccgca tgctgacggt tgaagaaaac gaacgcgcga
     3541 cacgcgtcgg ccccggaacc ccggccggaa aattgttccg gcgctattgg tggccgctat
     3601 gcctttccac cgaattgccc gagaaggacg gcgccccctt gcgtatccgg ctccttggcg
     3661 aagatctcac cgcgtttcgc gacaccgaag gcaaaatcgg cttgatcgat gcttattgcc
     3721 cgcaccggcg cgcgcctctg ttcttcgccc gcaacgagga atgtggcctg cgctgcgtct
     3781 atcatggctg gaaattcgac cggaacgggg attgcgtcga tatgccgtcg gagcccgccg
     3841 gtacgacgct gcag
//
LOCUS       DASYEG010000017         2488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4354230, whole genome shotgun sequence.
ACCESSION   DASYEG010000017 DASYEG010000000
VERSION     DASYEG010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4354230"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1740)
                     /gene="pheT"
                     /locus_tag="VGM72_00815"
     CDS             complement(<1..1740)
                     /gene="pheT"
                     /locus_tag="VGM72_00815"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY4263832.1"
                     /translation="MRFTLSWLKNPLDTQADVQAIADKLTNIGLEVESVEDAGARLKD
                     FIVAHVISANKHSNADKLKLCMVDTGAGEAVQVVCGAPNAHAGMKAVFAKPGTVIPAS
                     GAELKIGTIRGVESRGMLCSGRELLLSDDHDGIIALPADAIIGAPAAAALGLGDPVFD
                     VAITPNRGDCTSVYGIARDLAAAGLGRLREGDLSPVAGKFPSPIKTALDFPPGQENAA
                     PMFAGRVIRGVRNGPSPKWLQDWLKAVGLRPISALVDVTNFLSLDRGRPLHVFDTSKL
                     QGNLRARFAKDGETLAALDGKTYTLDSEMVVIADDQAARGIAGVMGGEDSSCTAETTD
                     VFIESALFDPVRVARTGRVLGILSDARYRFERGVDPEFVIPGLELASKMILQFCGGEA
                     SEIVAAGAPLPWKRTIEFPLSEVARLAGLALEPREIVRILTELGFVVKNAEGVLFADP
                     PSWRSDVHGATDLVEEVVRIHGLQNVPAVAMNRPYAVARAVLTPAQRREAFARRAAAA
                     HGFDECVHFSFIPRAHAVLFGGGDEARQLENPISADLDAMRPSLLPSLLAAVARNQAR
                     GFDHAMLFEIGAQF"
     gene            complement(1751..>2488)
                     /gene="pheS"
                     /locus_tag="VGM72_00820"
     CDS             complement(1751..>2488)
                     /gene="pheS"
                     /locus_tag="VGM72_00820"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEY4263833.1"
                     /translation="DEIIAILADLDFKVAEGPDIETDDYNFTKLNIPAEHPARQMHDT
                     FYLKPQADGSRPVLRTHTSPVQVRTMLSQKPPIRIIAPGRTYRSDSDATHAPMFHQVE
                     GLVIDEKTHLGHLKWTLQEFCKAFFEVPHVTMRLRPSYFPFTEPSVEVDIQCDRSGGQ
                     IRFGEGKDWLEIAGSGMVHPRVLEMCGIDPKRYQGYAFGFGIDRLAMLKYGIPDLRPF
                     FESDLRWLKHYGFAALDIPALHEGLSR"
BASE COUNT          495 a          767 c          772 g          454 t
ORIGIN      
        1 gaattgtgcg ccgatctcga acagcatcgc gtggtcgaag ccgcgcgcct ggttgcgcgc
       61 caccgcggca agcagagaag gaagcaaact cgggcgcatg gcatcgagat cggccgagat
      121 cggattttcc aattgccgcg cctcgtcgcc gccaccgaac aaaaccgcat gcgcgcgcgg
      181 tatgaacgag aaatgcacgc actcatcgaa gccgtgtgcg gccgctgcac gtcgcgcgaa
      241 cgcttcgcgc cgttgcgcag gcgtgagcac ggcgcgcgcc acagcatagg gacggttcat
      301 cgcgacggcc gggacgttct gcaagccgtg gatgcggacg acttcttcca ccagatcggt
      361 cgcgccatgc acgtccgacc gccatgaggg cggatcggcg aacagcacgc cttcggcatt
      421 tttgaccacg aaacccagtt ccgtcaggat acggacgatt tcgcgaggct ccaatgcgag
      481 gcctgccagc cgcgccacct ccgaaagcgg gaattcgatg gtccgcttcc agggtagagg
      541 cgcccctgcg gccacgatct cggacgcttc gccaccgcag aattggagga tcatcttgga
      601 ggcaagctcg agaccaggga ttacaaattc cggatcgacg ccgcgctcaa aacgataacg
      661 cgcatccgaa aggattccga gaacgcggcc cgtacgcgcc acgcgcaccg gatcgaacaa
      721 cgcggattcg atgaacacat cggtcgtttc agcggtgcag gatgaatcct cgccgcccat
      781 cacgccggca ataccgcgtg cggcttggtc atcggcgatg accaccattt ccgaatcgag
      841 tgtgtaggtc ttgccgtcca acgccgcgag cgtttccccg tctttcgcga agcgcgcccg
      901 caaattgcct tggagtttgc tcgtatcgaa gacatgcagc ggccgcccgc gatcgagcga
      961 caggaaattc gtgacgtcga ccagcgcgga aatcgggcgc aagccgacgg ccttgagcca
     1021 gtcctgcagc catttgggtg acggtccgtt cctgacaccc cggatcacgc ggccggcgaa
     1081 catcggcgcc gcgttctctt ggccgggcgg aaaatcgagc gccgtcttga tgggagaggg
     1141 gaatttccct gcgaccggcg acaggtcgcc ttcgcgcagg cggcctaatc cggccgccgc
     1201 caaatcgcgg gcaatgccat agacactcgt gcaatcgccg cgattgggcg tgatggcgac
     1261 gtcaaacacg ggatcgccga gaccaagcgc cgctgccgcc ggcgcgccga tgatcgcgtc
     1321 ggccggcagc gcgatgatgc catcatgatc gtcgctcaaa agaagctcgc ggcccgaaca
     1381 cagcatgcca cgcgattcca cgccgcggat ggtaccgatt ttcaattctg cgccgcttgc
     1441 gggaatgacg gtcccgggtt tcgcgaatac cgccttcatt ccggcatgcg cattgggtgc
     1501 gccgcagacg acctgaacgg cttcgccggc gccggtatcg accatgcaga gcttcagttt
     1561 gtcggcgttg gaatgtttgt tggccgagat cacatgagca acgatgaaat ccttcagccg
     1621 cgccccggca tcctcgacgg attccacctc caaaccgata ttggtgagtt tgtccgcaat
     1681 ggcctgcacg tccgcttgcg tgtccagcgg gttcttcagc caggaaagcg tgaatctcat
     1741 cgtacgcctc tcaccggctg aggccttcgt gcaacgccgg gatgtcgagt gcagcaaagc
     1801 cgtaatgctt gagccagcgc agatcggatt cgaagaaggg ccgcagatcg ggaatgccgt
     1861 atttcagcat cgcgagccgg tcgatgccga agccgaaggc atagccctgg tagcgtttcg
     1921 gatcgatgcc gcacatctcc agcacacgcg gatgcaccat gccggaccct gcgatctcca
     1981 gccaatcctt gccctcgccg aagcggatct gtccgcccga acggtcgcat tggatgtcga
     2041 cttcgaccga aggctcggtg aacgggaaat aggacggcct cagacgcatc gtcacgtgcg
     2101 gcacttcgaa aaaggctttg cagaattcct gcaaggtcca tttcagatgg ccgagatggg
     2161 ttttctcatc gatcaccagg ccttcgacct ggtggaacat gggcgcatgt gttgcatccg
     2221 aatcgctacg ataggtgcgc cccggcgcga tgatgcggat gggcggcttc tggctcagca
     2281 tggtgcgaac ctgcaccgga cttgtgtggg tgcgcagaac gggacgcgaa ccgtcagctt
     2341 gcggtttcaa atagaacgtg tcgtgcatct ggcgcgcggg atgttcggcc gggatgttga
     2401 gcttggtgaa attgtaatcg tcggtctcga tatcggggcc ttcggcgacc ttgaaatcga
     2461 gatcggcgag aatggcgatg atctcgtc
//
LOCUS       DASYEG010000018         6275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3050404, whole genome shotgun sequence.
ACCESSION   DASYEG010000018 DASYEG010000000
VERSION     DASYEG010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6275
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3050404"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2033)
                     /locus_tag="VGM72_00825"
     CDS             complement(<1..2033)
                     /locus_tag="VGM72_00825"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY4263834.1"
                     /translation="MAAIKDDDSAAQRSNRPKYFGAAVKRTEDPALLTGKGHYVDDIR
                     LPGTLHAAFVRSIHAHARIRSIDASAARALCGVHLVLTHADLPEAAQKPLTLLVPNPA
                     ITQLFLPMVLANTEVCYVGEPIAMIVADTRYIAEDAATLVAVEYDILPAISDCRDAIA
                     AAAAVAHEGTSSNIAGRIPFSHGDHDAAFAGAAHVFGESLHTHRGGPFFMECRGLVAS
                     FDAITDSFTIHISSQGSHRIKRCLLDALDLNDNQLRVITSDVGGGFGPKGALYPEYPC
                     VAVAAETLGRPVKWIEDRRENFVATHQERDEYWDMEIAVDANAKILGLRGRMIQDEGA
                     YLPWGLTVPWIAATTVPGPYVIPSLKMEVLVAFTNKISTTPVRGAGRPAGVFVMERLM
                     DRVARELNLDPGEVRRRNFVRPEQMPYKVGIIFRDGRPVTYDSGDYPACQATALTAAG
                     YDAFAARQSEARAKGRYLGIGIGNAVEATGLGPYEGVTVRISTTGKISLYTGATPHGQ
                     SHKTVLAQIAADELGADYEDIAVITGDTAGIAFGMGTFAARTAVNAGSSAHLAAKEVA
                     KKLKQIAAEMLQASPADIELNNGFAQMRDAPETRRHFRELAFKAAGMPGVSMAGGLTP
                     GIEHTAYFTPDQSTYSNGTHVAEVEVDVETGGVEILRYIVMHDCGRVINPMVVR"
     gene            2174..3817
                     /locus_tag="VGM72_00830"
     CDS             2174..3817
                     /locus_tag="VGM72_00830"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY4263835.1"
                     /translation="MTGRSRPALDFQDHLSALDAAGLLVRVDRPINKDTELHPLMRWQ
                     FLGGVPEDERRAFLFTHVVDSKGRRYDMPVAVGALAASPRIYAMGMGTQVNEIGPAWD
                     RAIAHPLPPVLVNHAPCQEVVIRGEDLTRPGGGLASLPVPISTPGFDSAPYLTATLCI
                     TRDPESRVQNMGMYRAALKATDRLGVRMVARPGGAGGYLHWQKYNALKEPMPIAIVIG
                     AAPIVCFTGPQKLAVDMDEMGVAGALAGEPISMVKCMSVDLDVPANAEIVIEGLIDPE
                     WLEPEAPFGESNGYVALEAFNMKMQVTAITMKRKPVFTAIISQVTPSESSVIKKVAYE
                     PFFLAHLRDQLEVTGIKRVVMHERLTNLRPVIFLQYAQGTPQIEVWRGLYATANFQSG
                     CGKIVVAVSEDIDPQSVDAVLWSLAYRFNPVEDVHVAPYHTSLQGSQYGPKKSDSTLL
                     IDATQKNPMPPLALPAREYMEHARGLWDELGLPPLSVTAPWHGYTLGDWDDTWDVFAA
                     RATRGDWEQSGKETLARARKGLKPETPVKTVEKNPGGET"
     gene            3829..5217
                     /locus_tag="VGM72_00835"
     CDS             3829..5217
                     /locus_tag="VGM72_00835"
                     /inference="COORDINATES: protein motif:HMM:NF037981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HEY4263836.1"
                     /translation="MATDASVEIAVRGDATPVPQAAARGLFDIGIHDRLPFGQALMLG
                     FQNIFGMIGMFVFPGITGQVLHLSVEQTAHLYGMTFLVSGFVTTCQSVLILKLPIVHG
                     PYVGSFTALLALGALPDAGLGLAFGSCFVACIIWFLLTVPIRGWSFAALFARYLHHPM
                     ISGIMVLLAMVQIANTSLPGWIGERASPGFPGINLMAGAVAVAILVCFTLWGGRRFRR
                     VAMLVGLVLGTLSYALFIPISFGRVMSSPWLVTPQFFPYGFEVRPDIVILFVLVLVPA
                     NIASMALYTVVGKWAHETLSPARMSGGLMSVALGGMLASVLGTYATHIYPDNMGLLRT
                     TRVASRYATLACGVLLMLLGSCVKFDMMLVLVPTPVLSAIATLLFGIVMMHAIQHLSG
                     VEWDDRNLIIAGFSLLLGMGGLFVTGDAYQALPLVVRILLKQAVVVGGIPLITLHAIL
                     NHDRIAAGHTKV"
     gene            complement(5277..>6275)
                     /locus_tag="VGM72_00840"
     CDS             complement(5277..>6275)
                     /locus_tag="VGM72_00840"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY4263837.1"
                     /translation="PDTPALVRSLVHYREPSHARALFELGVTAGALALFWVLMWLSLG
                     VGYWLTLLLSLPAAGFLVRLFLIQHDCGHGAFFRHRIANDWVGRILGVLTLTPYDYWK
                     RNHAIHHASSSNLDRRGIGDIETLTVQEYRERSWLGRCAYRIYRNPLVMFGVGPAYLF
                     FLQHRVPLNQMRAGWKPWLSTMATNVAIGLAVALVVCFVGLRPFFLVHLPIMLLAASI
                     GVWLFYVQHQYEGVVWTREHAWTRDESALSGSSHYNLPGVLRWFTANIGVHHVHHLNS
                     RIPYYRLPQVLRDHPELNDVSRLTLCQSLRGVRLALWDETRQRLVSFREMAGGYAR"
BASE COUNT         1180 a         1955 c         1948 g         1192 t
ORIGIN      
        1 cgcaccacca tcggattgat gaccctgccg caatcgtgca tcacaatgta gcgcaggatc
       61 tcgacgccgc cggtttcgac atccacctcc acttccgcca catgggtccc attggagtag
      121 gtcgattgat cgggcgtgaa ataggccgtg tgctcgatcc cgggcgtcag gccgcctgcc
      181 atcgacaccc ctggcatgcc ggccgccttg aaggccaatt cgcgaaaatg cctgcgcgtc
      241 tcgggagcat cgcgcatttg cgcgaaacca ttgttcagct cgatatccgc gggcgatgcc
      301 tgcagcattt cggcggcgat ctgtttcagt ttctttgcca cttctttcgc ggcgagatgc
      361 gccgatgaac cggcattcac cgcggtacgc gccgcgaacg tccccatccc gaacgcgatg
      421 ccggcagtat cccctgtgat cacggcgatg tcttcgtaat ccgcacccag ctcatcggcg
      481 gcgatctgcg cgagaacggt cttgtgcgat tggccgtgcg gggtcgcgcc ggtgtagagg
      541 ctgatcttgc cggtggtgga gatgcgcacc gtcacgcctt cataggggcc gagcccggtg
      601 gcctccaccg cattgccgat accgatgccg agatagcgcc ccttcgcgcg cgcctcactc
      661 tgtcgtgccg cgaaagcgtc atagccggcc gccgtcagcg cggtcgcctg gcatgcggga
      721 tagtcgccgc tgtcataggt gaccgggcgt ccgtcgcgga agatgatgcc gaccttgtag
      781 ggcatctgtt cggggcggac gaagttgcgc cgccgcactt cgcccggatc gaggttcagc
      841 tcccttgcca cgcgatccat cagccgctcc atcacgaaga cgcccgcggg ccggcccgcg
      901 ccgcgcaccg gcgtcgtgga gatcttgttg gtgaaggcga caagcacctc catcttcagg
      961 ctcggaatga cataaggccc gggcacggtg gtggccgcga tccacggcac cgtcaggccc
     1021 cagggaagat aggcgccttc gtcctgaatc atacggccgc gcaagcccag gatcttcgcg
     1081 ttggcgtcca ccgcgatctc catgtcccaa tattcgtcgc gctcctgatg ggtggcgacg
     1141 aaattctcgc gccggtcctc gatccatttc accggccggc cgagcgtttc cgcggccacg
     1201 gcgacgcagg gatattcggg atagagcgcg cccttgggac cgaagccgcc gccgacatcg
     1261 gaggtgatga cgcgaagctg gttgtcgttc agatcgagcg catccaggag gcagcgcttg
     1321 atgcgatgcg agccttgcga ggagatatga atcgtgaagc tgtccgtaat ggcatcgaag
     1381 cttgccacca gaccgcggca ctccatgaag aatgggccgc cgcgatgggt gtggagactt
     1441 tcgccgaaca catgggcggc tccggcgaag gccgcatcgt gatcgccatg actgaaggga
     1501 atgcggccgg cgatgttgga cgatgtgcct tcgtgggcca ccgccgctgc cgccgcaatc
     1561 gcgtccctgc aatcggaaat cgcgggcaga atgtcgtact cgaccgcgac gagagtggcg
     1621 gcatcctcgg cgatataacg cgtgtcggcc acaatcattg cgatgggttc gccgacatag
     1681 cagacctcgg tgttcgcgag caccatcggc agaaagagct gggtgatcgc cggattcggc
     1741 accagcagcg tcagcggctt ctgcgccgcc tcgggaagat cggcatgggt gagcaccaga
     1801 tgcacgccgc aaagcgcgcg cgccgccgac gcatcgatgc ttctgatccg cgcatgggcg
     1861 tggatgctgc gcacgaaggc ggcatgcaat gttccgggca atcggatgtc gtcgacataa
     1921 tggcctttgc cggtgaggag ggccggatct tccgtgcgct tgaccgcggc gccgaaatat
     1981 ttcggacgat tggagcgttg ggcggcgcta tcatcgtcct taatcgctgc catcgcgtcc
     2041 tcatgcccgg ccatcattcg gccaggcatg gaataaacgc tctgcccatc tttgcaaagt
     2101 ggtcgaagcg ctccattgta gcgtcacgct gcggcggaaa acgcagggat tctgggagca
     2161 aagacaaata acaatgaccg gccgttcacg cccagcgctt gatttccagg atcatctttc
     2221 cgccctcgac gccgcgggcc tgctcgtccg ggtggaccgc ccgatcaaca aagacaccga
     2281 actgcatccg ctgatgcgct ggcagttcct tggcggcgtt ccggaagacg agcgccgggc
     2341 ctttttgttc acccatgtgg tcgattccaa ggggcggcgt tacgacatgc cggtggcggt
     2401 gggcgcgctg gccgcctctc cacgcatcta tgcgatgggg atgggtacgc aggtgaacga
     2461 gatcggcccc gcctgggacc gggccattgc ccatccgctg ccgccggtcc tggtcaacca
     2521 cgcaccgtgt caggaggtcg tgatccgtgg cgaggatttg acgcgcccgg gcggtggtct
     2581 cgcgagcctg ccggttccca tatccacgcc cggcttcgat tccgcgccct acctgacggc
     2641 gacgctctgc atcacgcgcg atcccgaaag ccgcgtgcag aacatgggca tgtatcgcgc
     2701 cgcgctgaaa gcgaccgacc ggctgggcgt gcgcatggtg gcgcgtccgg gcggcgcggg
     2761 cggctatctg cactggcaga aatacaacgc gctgaaggag ccgatgccca tcgccatcgt
     2821 gatcggcgcc gcgcccatcg tctgtttcac cgggccgcag aagctcgccg tcgacatgga
     2881 cgagatgggc gtcgccggcg ctctcgcggg tgaaccgatt tccatggtga aatgcatgag
     2941 cgtcgatctg gacgttccgg cgaacgcgga aatcgtcatc gaaggcctga tcgatcccga
     3001 atggctggag cccgaagcgc cgttcggcga aagcaacggt tatgtcgcgc tcgaagcctt
     3061 caacatgaag atgcaggtga cggccatcac catgaagcgc aagccggtgt tcaccgccat
     3121 catcagccag gtgacgccgt ccgaatccag cgtcatcaaa aaggtcgctt atgagccatt
     3181 cttcctggcc catctgcgcg accaattgga agtgacaggc ataaagcgcg tggtgatgca
     3241 tgagcggttg acgaatttgc gccccgtcat cttcctgcaa tatgcgcaag ggacgccgca
     3301 gatcgaagtg tggcgcgggc tttatgccac cgcgaatttc cagagcggct gcggcaagat
     3361 cgtagtggcg gtgagcgaag atatcgaccc gcagagcgtc gatgcggtgc tgtggtcgct
     3421 cgcctaccgc ttcaacccgg tcgaggacgt gcatgtcgcg ccctatcaca ccagcctgca
     3481 gggttcgcaa tatggcccga agaaatccga ttccacccta ttgatcgatg cgacccaaaa
     3541 gaatccgatg ccgccgctgg cattgcccgc gcgcgaatat atggaacatg ctcgcgggct
     3601 ctgggacgag ttgggactgc cgccgttgag cgtcaccgcg ccctggcacg gctatacgct
     3661 cggcgattgg gacgacacct gggatgtgtt tgcggcccgc gcgacgcgcg gcgactggga
     3721 gcaaagcggc aaggaaacgc tcgcgcgcgc gcgcaaaggt ctgaaacccg aaacgccggt
     3781 gaaaacggtg gagaaaaatc cgggcggcga aacctaggga cgcggcgcat ggcgacggac
     3841 gcaagcgtgg agattgccgt gcgaggggac gcaacgcccg tccctcaagc cgcggcgcgc
     3901 ggcctgttcg atatcggcat ccacgatcgc ctgcccttcg gccaggccct gatgctgggc
     3961 tttcagaaca ttttcggcat gatcggaatg ttcgtgtttc ccggcatcac cggacaggtg
     4021 ttgcatctct ccgtcgaaca gaccgcccat ctttacggca tgacattctt ggtttcgggc
     4081 ttcgtcacca cctgccaatc ggtgctcatc ctgaaacttc ccatcgtgca cggcccctat
     4141 gtcggcagct tcaccgcgct gctggcgctg ggcgcgcttc ccgatgccgg tctcgggctt
     4201 gccttcgggt cgtgcttcgt tgcctgcatc atatggttcc tgctcaccgt gcccattcgc
     4261 ggttggagct ttgccgcgct tttcgcgcgc tatcttcacc acccgatgat atcggggatc
     4321 atggtcctgc tggcgatggt gcagatcgcg aacacctcgc ttcccggctg gatcggggag
     4381 cgggccagtc ccggatttcc cggcatcaac ctgatggccg gcgcggtcgc cgttgcgatc
     4441 ctcgtgtgtt tcacgctctg gggcggcagg cggttccggc gcgtggccat gctcgtaggc
     4501 ctcgtgctgg ggacattgtc ctatgcgctg ttcatcccga tttcattcgg acgcgtgatg
     4561 tcgtcgccct ggctggtgac gccgcaattc tttccctatg gcttcgaggt ccggcccgat
     4621 atcgtgatcc tgttcgtgct ggtgctggtg ccggccaata tcgcatccat ggcgctctac
     4681 accgtggtcg gcaaatgggc ccatgagacg ttaagccccg cccgcatgtc aggcgggttg
     4741 atgagcgtgg cgctgggcgg catgctggct tcggtgctcg gcacttatgc gacgcacatc
     4801 taccccgaca atatgggcct gttgcgcacc acccgcgtgg cttcgcgtta cgccaccctt
     4861 gcctgcggcg tgctgctgat gctgctggga tcttgcgtga aattcgacat gatgctggtg
     4921 ctggtgccga cgccggtcct ctctgcgatc gcgacgctgc tgttcggtat cgtgatgatg
     4981 catgcgatcc agcatctgtc gggagtcgaa tgggacgacc gcaacctgat catcgccgga
     5041 ttttcgctgc ttctgggcat ggggggcctg ttcgtcacag gcgatgccta tcaggcgctg
     5101 ccgcttgtgg tgcggatatt gctcaaacag gcggtggtcg tcggaggcat acccctgatc
     5161 acgctgcacg caattctgaa ccatgacaga atcgcggcgg gccatacgaa ggtctgacgc
     5221 cccgcagaaa tgggccttcc caatttctcc ggaaaaaaga aaagctcgca acaatgtcag
     5281 cgagcgtatc ccccggccat ttcccgaaaa gagacgagtc tctgccgcgt ctcgtcccac
     5341 aacgccagac gcacgccgcg taaactctgg cataacgtca atcggctgac atcgttaagc
     5401 tccgggtggt ctcgcagaac ctgcggcagc cggtaatagg gaatacggct gttcaggtga
     5461 tgaacatggt gcaccccgat attggcggtg aaccaacgca acacgccggg aagattgtag
     5521 tgcgagcttc ccgacagagc cgattcgtca cgagtccagg catgttcccg cgtccagacg
     5581 acgccctcat attgatgctg gacgtaaaac agccaaacgc cgattgaggc cgccaaaagc
     5641 atgatcggca gatgcacgag aaaaaagggc cggagcccca caaaacacac cacgagagcc
     5701 acggcgagac caattgcgac gttggttgcc atcgtgctca gccagggctt ccagccagca
     5761 cgcatctgat tgagcgggac ccggtgctgc aagaagaaga gataggccgg ccccacgccg
     5821 aacatgacga ggggattgcg atagatccga taagcgcatc gccccagcca cgatctttcc
     5881 ctgtattcct gcaccgtcag ggtttcgatg tcgccaatgc ccctgcggtc gagattgctc
     5941 gacgaggcgt gatggatcgc gtgatttcgc ttccaataat cgtatggcgt caatgtgaga
     6001 acgccgagta tccggccaac ccagtcattc gcgatgcgat ggcgaaaaaa tgcgccatga
     6061 ccgcaatcgt gctgaatcaa gaacagtcgc accagaaacc cggcagccgg cagggacagc
     6121 agcaaggtca gccaatagcc aacgccaagc gacagccaca tcaagaccca aaacagcgca
     6181 agcgccccgg cggtgacacc gagttcaaac aaagcacgcg cgtgacttgg ttctcggtaa
     6241 tgaacgagac ttcgcaccag cgctggcgta tcggg
//
LOCUS       DASYEG010000019         2386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3775634, whole genome shotgun sequence.
ACCESSION   DASYEG010000019 DASYEG010000000
VERSION     DASYEG010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3775634"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VGM72_00845"
     CDS             complement(<1..815)
                     /locus_tag="VGM72_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY4263838.1"
                     /translation="MSPSKHIVPDAVLSRAEPLPEAERLARVPSTPFAFMRFLIGRHF
                     RWKIAALVTLAGSATSIEAFGPYALSHLINAINAAVAAHASFATAILPWMLLLAVIWL
                     GSTLAYRAYEAVDVTTSPRMRALAQKYLFTYLMGHSPRYFQENFAGKLGQKVKQAGQA
                     TVSVLNILAFEMVRVAMLMLVGGILMFNAHASYALLLGVWAAIYLAIVVSLARRCVVL
                     SKALSNEVSTSTGRLIDAIANSDLVRAFAKADYERRFISHFLADEMNASRRLRT"
     gene            complement(923..1345)
                     /gene="rplS"
                     /locus_tag="VGM72_00850"
     CDS             complement(923..1345)
                     /gene="rplS"
                     /locus_tag="VGM72_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEY4263839.1"
                     /translation="MNLIQTLEREQMDASRGGRKLPIFRPGDTLKVNVKVVDVSYDEK
                     TKQQKTRERLQAFEGVCIARQGAGLNESFTVRKISYGEGVERVFPIYSPLVDSVEVIR
                     HGRVRRAKLYYLRGLRGKAARIKERQHVAEIETAGAGA"
     gene            complement(1323..2108)
                     /gene="trmD"
                     /locus_tag="VGM72_00855"
     CDS             complement(1323..2108)
                     /gene="trmD"
                     /locus_tag="VGM72_00855"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEY4263840.1"
                     /translation="MWRASVLTLFPDMFPGPLGASLIGKARGEGIWNLDVCDIRDHGM
                     GRHRAVDDTPSGGGPGMVIRSDVAATAIDAARGQDTAPRPLIYLSPRGAPLTQARIRE
                     FSAKPGIVLFCGRFEGLDERAIEARGMEEISMGDFVLAGGEIAAMALIEAVVRLLPGV
                     MGAPASKTEESFEQGLLEYPQYTRPQSFEGRDIPPVLMSGNHAEIARWRKAEAERLTK
                     ERRPDLWTLYEKFARSARRPASLDRRQAKGPPKDAKNEPDSDP"
BASE COUNT          473 a          767 c          743 g          403 t
ORIGIN      
        1 gtgcgcaacc gccgcgaggc gttcatctcg tcggccagga aatgcgagat gaagcggcgt
       61 tcgtaatcgg ccttggcgaa ggcgcgcacg agatcggaat tggcgatggc atcgatcaac
      121 cgccccgtcg aggtggagac ctcgttggac agcgccttgg acagaaccac gcaacgccgc
      181 gcgaggctga ccacaatggc gagatagatc gcagcccaaa caccgagcag cagcgcataa
      241 ctcgcatggg cgttgaacat cagaatgccg ccgaccagca tcagcatggc gacgcgcacc
      301 atttcgaagg ccaggatgtt gagcacggaa accgtcgcct gaccggcctg tttcaccttc
      361 tgaccgagct tgccggcgaa gttttcctga aaatagcgag gcgaatggcc catcagatag
      421 gtgaaaagat atttctgcgc gagcgcgcgc atgcgcgggg atgtcgtgac gtcgacggct
      481 tcgtaagcac gataggcaag cgtggaaccg agccagatga cggcgagcag cagcatccag
      541 ggaaggatcg cggttgcaaa actcgcatgg gccgccaccg ccgcattgat cgcgttgatg
      601 agatgggaca acgcataggg tccgaacgcc tcgatcgagg tcgccgatcc cgcgagcgtc
      661 acgagcgcgg caatcttcca gcggaaatgc ctgccgatca gaaagcgcat gaaggcaaac
      721 ggggtggacg gcacgcgcgc caatcgctcg gcttcgggca ggggctcagc cctgctcagc
      781 acggcatcgg gcacaatatg ttttgacggg ctcatggccc aatcctcaaa agggggatcg
      841 ccccaaacgg ctccggccgc gcggcgcgcg attcgaagtc cgcatcatag gtggaaaata
      901 agtccggtcc ggtaaagaag acctaagcgc cggcacccgc ggtttcgatt tccgccacgt
      961 gctggcgctc tttgatgcgg gcggctttgc cgcgtaggcc gcgcagataa tagagcttcg
     1021 cgcgccgcac ccggccatga cggatcactt ccaccgaatc caccagcggg ctgtagatcg
     1081 ggaagacccg ctccacgccc tcgccatacg aaatcttccg gaccgtgaag ctttcgttca
     1141 ggcccgcacc ctgccgcgcg atgcagacgc cctcgaaggc ctgaagacgt tcgcgggtct
     1201 tctgctgctt ggttttctcg tcataggaca cgtcaacgac cttgacgttg accttcagcg
     1261 tgtcccctgg ccggaaaata ggcagcttgc ggcccccacg ggaggcatcc atttgttcgc
     1321 gctcaagggt ctgaatcagg ttcatttttc gcatccttcg gcgggccctt ggcctgcctg
     1381 cggtctaaag aggcgggcct tctagcggat cgggcgaact tttcatagag ggtccagaga
     1441 tcgggccggc gctctttcgt gagccgttcg gcctccgcct tccgccagcg ggcgatttcg
     1501 gcatgattgc cggacatcag gacaggggga atgtcgcggc cttcgaagct ttggggccta
     1561 gtgtactgcg gatattcgag gagcccttgt tcaaaacttt cctcggtctt ggaggctggc
     1621 gcgcccatca cgccgggaag gagccggaca accgcctcga tcagcgccat ggcggcgatc
     1681 tcaccgccgg caaggacaaa atcccccatg gagatttcct ccatcccccg ggcttcgatc
     1741 gcccgctcgt ccagcccctc gaaccggcca cagaaaagga cgattccggg tttggccgaa
     1801 aattcccgta tgcgggcctg cgtcaacggg gccccgcgcg gcgagaggta gatcagcggc
     1861 cggggggccg tgtcctgacc ccgcgccgca tcgatggcgg ttgccgcgac gtcggagcgt
     1921 atcaccatgc cggggccgcc acccgaaggg gtatcgtcca cggcgcgatg acgtcccatc
     1981 ccatggtcgc ggatgtcgca gacatccaga ttccagattc cctcacccct ggccttgccg
     2041 atcagcgaag cccccagcgg gccggggaac atgtccggaa acagggtcag gaccgaagca
     2101 cgccacattc aagatcacct gtcatcccgg ccagcccccg ggtccgggtg gagccggccc
     2161 aagggtaaac tccacgaagc gcgtgccgcg agccacccag aaacgggccc ggtgggtctc
     2221 tggctccccc caaccttggc ctcaactaag gctggctcgc ccaggatgac aagggagttg
     2281 ggtcagttct ggttctcacg cggcagttcg atcacgatac gcccgcccgc gacatccacc
     2341 accggcacat gcgggtcggc gaaggcgacg aactccgtgc ccccgg
//
LOCUS       DASYEG010000020         5735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_877478, whole genome shotgun sequence.
ACCESSION   DASYEG010000020 DASYEG010000000
VERSION     DASYEG010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877478"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VGM72_00860"
     CDS             complement(<1..348)
                     /locus_tag="VGM72_00860"
                     /inference="COORDINATES: protein motif:HMM:NF018264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HEY4263841.1"
                     /translation="MTTTPHLIESLAADAKPVRRLRPPLARASAWLAGFVAIVGIVTW
                     TTGAWPAMMARLDDMRFAVELTATFVTGLAAVIAAFHLSLPDRSRVWMLLPVPPLVLW
                     LASSGYGCYRNWVA"
     gene            complement(345..920)
                     /locus_tag="VGM72_00865"
     CDS             complement(345..920)
                     /locus_tag="VGM72_00865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY4263842.1"
                     /translation="MSDAAQLRGEDDWAGLMARAQAGDAVSYRRLLIAVTPYLRAIAA
                     RSHRERHDVEDSLQDILLTVHAVRHTYDPARPFKPWLAAIARRRIIDRLRGQGRKRAR
                     ETFLSEEHETFAAPETNLTEAEPDFRALRAAVEQLPEGQRQAITMLKIEEKSLKEASA
                     ASGMSIVALKVSTHRAVKNLRKLLDRRRDGI"
     gene            1054..1353
                     /locus_tag="VGM72_00870"
     CDS             1054..1353
                     /locus_tag="VGM72_00870"
                     /inference="COORDINATES: protein motif:HMM:NF021559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HEY4263843.1"
                     /translation="MKNMTAAAALTSAIGLAFALQAAPTRAAEMPQIVKDNMAMAAKN
                     HLEKCYGINANAKNDCAEGAHSCAGQATMARDPKSFVLLPAGVCGKIQGGKTTAS"
     gene            1391..2260
                     /locus_tag="VGM72_00875"
     CDS             1391..2260
                     /locus_tag="VGM72_00875"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEY4263844.1"
                     /translation="MPQAERGPIPAEVGIGLRFPHHRHVMDTRPDTAWFEVHTENYMG
                     GGTPLRVLEAIRRDYPISLHGVGLSLGSADGLDAAHLAHVRDVAERIEPGLISEHLSW
                     SIAGGIYLADLLPLPMTEEALHVTCRNVAQVQEILGRKILIENPSSYLQFTHSTIPEW
                     EFISAVAARTGCGILCDVNNVHVSACNHGWDASAYLAALPTDAIGEIHLAGHSVKEIG
                     GRILRIDDHGARVSPEVWALFREALERFGRVPALIEWDTNIPAFDVLREEASLAGYAM
                     QQSQEARPNAHAA"
     gene            2244..3044
                     /locus_tag="VGM72_00880"
     CDS             2244..3044
                     /locus_tag="VGM72_00880"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEY4263845.1"
                     /translation="MPTLRETQHALLNALAFGNSEHAASQIAGGGFSPDERLDIYRNT
                     YLSALTSALRISYPAVHRLVGAAFFEGAAQSFVETHPPRSAYLNVYGVEFAEFLAQFP
                     PAQSLPYLGDVARLEWAVNVALHAKDAAPMDPHIFARLGEIAPERLILVPHPAVTLLR
                     TDHAVRALWQAVLAEDDCALDAVDWAAGPEWLLVERTAAGIEVLCLPEREWRFTAALC
                     AGESFATAVEAAPGADVTTLFARHLATGCFTDFRRAGAYPLAPSETIP"
     gene            3041..3505
                     /locus_tag="VGM72_00885"
     CDS             3041..3505
                     /locus_tag="VGM72_00885"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY4263846.1"
                     /translation="MTQSSTLAPPHSPVLSTLVLLRATLQSVPYSLLALPLRFAVATV
                     FWNSGTTKLASWDTTLQLFADEYRLPLLPPELAATMAASIELTTPFLLVFGFLTRPAA
                     FILLGMTTVIEIFVYPLAWPTHIQWAAMLLVLLCRGPGRLSLDHLIWRWLRL"
     gene            3576..4538
                     /gene="mdh"
                     /locus_tag="VGM72_00890"
     CDS             3576..4538
                     /gene="mdh"
                     /locus_tag="VGM72_00890"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY4263847.1"
                     /translation="MSRNKIALIGGGQIGGTLALLIGLKDLGDVVLFDIVEGLPQGKT
                     LDIAQSAPVEGFDAKFKGTNSYADIAGADVVIVTAGVPRKPGMSRDDLLGINLKVMSA
                     VGEGIKAHAPNAFVICITNPLDAMVWALRQFCGLPHNKVVGMAGVLDSARFRHFLAEE
                     LNVSVEDVSAFVLGGHGDTMVPMPRFSTVAGIPLPELVKMGWITQARLDQLTQRTRDG
                     GAEIVGLLKTGSAFYAPASSAIAMAESYLRDKKRVLPCAAYVDGKYGLKDIYVGAPVV
                     IGAGGVERVVELDLTKDERAMFDKSVGSVQGLIEACRKLEPRLG"
     gene            4608..4683
                     /locus_tag="VGM72_00895"
     tRNA            4608..4683
                     /locus_tag="VGM72_00895"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4641..4643,aa:Thr,seq:cgt)
     gene            complement(4749..5138)
                     /locus_tag="VGM72_00900"
     CDS             complement(4749..5138)
                     /locus_tag="VGM72_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263848.1"
                     /translation="MTEIAATRLSRFGLSADGSACLVEFEKSDGETGRLTFPPAQLDE
                     VIGLLLQLKQVYAQKIEGNETRSVLVADRVGVLVQSDAAVLDFVVGGAPISFAIPLEM
                     AAQLTQILQERVPAPDARAARPPQTKA"
     gene            complement(5328..>5735)
                     /locus_tag="VGM72_00905"
     CDS             complement(5328..>5735)
                     /locus_tag="VGM72_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4263849.1"
                     /translation="AADQAHAHEFIAGRASGYDTIVGEQGIKLSGGERQRVAIARALV
                     KDARILVLDEATSSLDSESEHLIQEALWTLMQGRTVIAIAHRLSTIAGMDRIVYLEAG
                     RIVEDGPHRELLAKGGAYARLWNRQMGGFINAA"
BASE COUNT         1032 a         1851 c         1744 g         1108 t
ORIGIN      
        1 ggcgacccaa ttgcgatagc agccatagcc gctgctcgcg agccacagaa ccaagggcgg
       61 tacggggagg agcatccaga cccggctgcg gtcgggcagg ctgagatgga acgccgcaat
      121 caccgcagcc aaacccgtca cgaacgtggc ggtcagctcg acggcaaaac gcatgtcgtc
      181 cagccgtgcc atcatcgcgg gccaggcgcc cgtggtccac gtcacaatgc cgacaatcgc
      241 cacaaacccg gccagccacg cggacgcccg cgccaaaggc gggcgcaagc gccggacggg
      301 cttcgcatcc gcagcaagcg attcgatcag atgcggcgtc gtggtcaaat gccgtctctc
      361 cgccgatcca gcaatttgcg caaattcttc accgcccgat gtgtcgagac cttcagcgcg
      421 acgatggaca tcccactcgc ggcggatgcc tccttcaacg atttttcttc gatcttcaac
      481 atggtaatcg cctggcgctg tccttccggc aattgttcca cggcagcccg cagagcgcga
      541 aagtcgggct ccgcctcagt caggttcgtt tcaggagcgg cgaaggtttc atgctcctcc
      601 gaaagaaatg tttcacgggc gcgcttgcgg ccctgccccc gcagccggtc tatgatccgc
      661 cgccgcgcga tggcggcgag ccagggcttg aacggacgcg ccggatcata ggtgtgccgc
      721 accgcatgca cggtcaacag gatgtcctgt agggaatctt cgacatcgtg ccgttcacgg
      781 tggcttcgcg ctgcaatcgc ccgaagatag ggtgtcaccg cgatgagcag acgccggtag
      841 gagaccgcat cgcccgcctg tgcacgcgcc atgagcccgg cccaatcatc ttcgccgcga
      901 agttgcgctg catcgctcac ggcaaatccc tacactggaa acgcaaagag cggcagcaga
      961 tttatttccg gctgaagccc gtaacctttc aaaggcccca tccgaacctg ttgctgtccg
     1021 tctcgaagag agaccccaac aggagagccc atcatgaaaa acatgaccgc cgcggccgcg
     1081 cttacgtcgg ccattggcct tgccttcgcc ctacaggccg cgcccacccg agccgctgaa
     1141 atgccgcaaa tcgtaaaaga caacatggcg atggcggcca agaaccatct ggaaaaatgt
     1201 tacggcatca acgccaacgc gaaaaacgat tgcgccgagg gcgcgcattc ctgcgccgga
     1261 caggcgacca tggcgcgcga tcccaaatcc ttcgtccttc tgcccgccgg tgtctgcgga
     1321 aagatccagg gcggcaagac gacggcgtcc tgatccgatg gcgcggcagt ccttccctcc
     1381 cagacttgcc gtgcctcaag cggagcgcgg cccgattccc gctgaagtcg gaatcgggct
     1441 gcgtttcccg catcaccgcc atgtcatgga cacgcgcccc gataccgcct ggttcgaggt
     1501 gcataccgaa aactacatgg gcggaggaac acccctgcgc gtgcttgagg ccatacggcg
     1561 ggattatccg atctcgctgc acggtgtcgg cctatcgctc ggaagcgcgg acggtctcga
     1621 cgcggcgcat ctcgcacatg tccgcgatgt ggcggagcgc atcgagcctg gtttgatttc
     1681 ggagcatctg tcctggagca ttgcgggcgg aatctatctt gccgatctcc tgccgcttcc
     1741 catgaccgaa gaggcgctgc atgtgacatg ccgcaatgtc gcgcaggtcc aggagatcct
     1801 gggccgcaaa atcctgatcg agaatccgtc ttcttatctg caattcacac attccacgat
     1861 tccggaatgg gagttcatct cggccgtggc ggcgcgcacc gggtgtggga ttctctgcga
     1921 cgtgaacaat gtccatgtca gcgcctgcaa tcatggctgg gatgcctcgg cctatcttgc
     1981 cgcgctgcca acagatgcca ttggcgagat ccatctcgcg gggcacagcg tcaaagagat
     2041 cggtggacgt atcctgcgca tcgacgatca cggcgcgcgc gtcagtcccg aagtctgggc
     2101 gctctttcgc gaggcgttgg aacgcttcgg acgagtgccg gcgctgatcg aatgggacac
     2161 gaacatcccc gccttcgatg tcttgcgcga ggaagcgtcc ttggccgggt acgccatgca
     2221 gcaaagccaa gaggcacgtc caaatgccca cgctgcgtga gacccagcac gcgctgctga
     2281 acgcgctcgc cttcgggaac agcgagcacg ccgcatcgca gatcgcgggt ggcggatttt
     2341 cgccggacga acggctggac atctatcgca acacctatct gtcggcattg acgtcggcgc
     2401 tgcgtatttc ctatcccgcg gtgcatcgcc tggtcggtgc ggcattcttc gagggcgcgg
     2461 cgcaatcttt cgttgaaacg catccgccgc gcagcgccta cctgaatgtc tatggcgtgg
     2521 agttcgccga gtttcttgcg caattcccgc cggctcaatc gcttccctat cttggcgatg
     2581 tcgcacggct ggaatgggcg gtgaatgtcg cgctccacgc caaggacgca gccccgatgg
     2641 acccgcatat cttcgcgcgc ctcggcgaga ttgcgcccga acggctcatc ctcgtgccgc
     2701 atcccgcggt cacgcttctc agaaccgatc acgcggtgcg ggccctgtgg caggccgtgc
     2761 tcgccgaaga cgattgcgcg ctcgatgccg tcgattgggc ggccggtccc gaatggcttt
     2821 tggtcgagcg cacggcagcg gggatcgagg tgctctgcct tcccgaacgc gaatggcgtt
     2881 tcaccgccgc gctctgtgcc ggagaaagtt tcgcgacggc cgtcgaagcg gcgccgggcg
     2941 ccgatgttac gacactgttc gcgcgtcatc ttgccaccgg ttgcttcacc gatttccgcc
     3001 gcgccggcgc ttaccccctt gctccctcgg agacgattcc atgacccaat cttccaccct
     3061 cgcgccgccg cattccccgg tgctttccac tctcgtgcta ttgcgcgcga cgttgcagtc
     3121 cgttccctat tcgctgctgg cattgccgtt gcgtttcgcc gtggcgacgg tgttctggaa
     3181 ttccggcacc acgaagcttg caagctggga caccacgctc cagctcttcg ccgacgaata
     3241 caggctgccg ctgctgccgc ccgaacttgc cgccaccatg gccgcgtcca tcgagctgac
     3301 gacgccgttc ctgctggtgt tcggattcct cacccgcccg gcggccttca tcctcctcgg
     3361 catgacgacg gtgatcgaga ttttcgtgta tccgttggcc tggcccaccc acattcagtg
     3421 ggcggccatg ctgctggtcc tgctctgccg cggaccgggc aggctctcgc tggatcatct
     3481 gatctggcgg tggctgagat tgtaactccc ggcttgcccg gcgggcctgt ggccgttact
     3541 aacgcgcacc cggccctgca cgaaaaggag ttcccatgtc ccgcaacaaa attgcgctga
     3601 tcggcggcgg ccagatcggc ggcacgctgg cgcttctgat cggtctgaag gatctgggcg
     3661 acgtcgtcct gttcgatatc gtcgaggggc tgccgcaggg caagacgctc gacatcgcac
     3721 aatccgctcc ggtggaaggt ttcgatgcca agttcaaggg caccaattcc tatgccgata
     3781 tcgcgggtgc cgatgtggtg attgtgacgg ccggtgtgcc gcgcaagccc ggcatgagcc
     3841 gcgacgatct tctgggcatc aatctcaaag tgatgagcgc ggtcggcgaa ggcatcaagg
     3901 cccatgcgcc gaacgccttc gtcatctgca tcaccaatcc gctcgatgcg atggtgtggg
     3961 cattgcgcca attctgcggc ctgccgcaca acaaggtggt tggcatggcg ggcgttctcg
     4021 attccgcgcg cttccgccat ttcctcgccg aagaacttaa cgtctcggtc gaggacgtgt
     4081 ccgcctttgt gcttggcggt catggcgata ccatggtgcc gatgccgcgt ttctcgacgg
     4141 ttgccggcat ccccctgccg gaactggtga agatgggctg gatcacccag gcgcggctgg
     4201 atcagctgac ccagcgtacg cgcgacgggg gtgcggagat cgtgggtctc ttgaagaccg
     4261 gttccgcgtt ttacgcgccg gcgtcttcgg ccatcgccat ggcggaatcc tatctgcgcg
     4321 acaagaagcg cgtgctgccc tgcgcggcct atgtcgatgg taaatatggc ctcaaggaca
     4381 tctatgtcgg tgcgccggtg gtgatcggcg ccggcggcgt ggagcgcgtc gtcgagctcg
     4441 acctcaccaa ggacgagcgg gcgatgttcg acaaatccgt cggctcagtc caggggctga
     4501 tcgaagcctg ccgcaaactt gagccccggc tcggctaggc ggataagcct cttgtcctag
     4561 gcgggttgcg cgaaagaacg gacccgccta ttttccgcgc gcctttggcc gccttagctc
     4621 agccggtaga gcacgtcatt cgtaatgacg gggtcgcgtg ttcgagtcac gcaggcggca
     4681 ccaacgcttc gggctcgcta gaaaattccg gcgagcccga taacgatctt gtatccgttc
     4741 gcgcgaggct acgcttttgt ctgtggcggc cgtgccgctc gtgcatcggg cgcggggacg
     4801 cgctcttgga gaatctgcgt gagctgagcg gccatctcca gcggaatggc aaatgagatc
     4861 ggcgctccgc ccaccacgaa atccagcacc gccgcatcgg actgaaccaa tacgccaacg
     4921 cggtccgcga ccaggaccga gcgcgtttcg ttgccttcta ttttttgggc gtagacctgc
     4981 ttcaattgca ggagcaagcc gatgacctcg tccaattgcg cagggggaaa ggtgaggcgg
     5041 ccggtttcgc catcggattt ctcgaattcc acaaggcagg ccgaaccatc ggccgagagg
     5101 ccaaagcgcg aaagtcttgt agcggcgatt tcggtcatga aggctcgcaa aatgtggacg
     5161 cgcggcttct atagcagata atcccaatca ggctgtattg acatgctgat aacgcggctt
     5221 gcgacagcgt gcggtagaaa aaccctaaga acacaaagtg agcgcgaaga acacaaagca
     5281 cttcgtgcgc ttggtgctcc ttcgtgtcct tcgtggtgtt ttttttctca cgccgcgttg
     5341 atgaagccgc ccatctgacg gttccataac cgcgcgtagg cgccgccctt ggcgagcagc
     5401 tcgcgatgcg gtccgtcctc gacgatgcgt ccggcctcca gatagacgat gcggtccatc
     5461 ccggcgatgg tcgagagccg gtgggcgatg gcgatcacgg tgcgcccctg catcagcgtc
     5521 cacaacgctt cctggatcag atgttccgat tccgaatcga gcgaagaagt cgcctcgtcc
     5581 agcaccagga tgcgcgcatc cttcaccaaa gcgcgcgcga tcgcgacgcg ctggcgttcg
     5641 ccgcccgaaa gcttgatccc ctgctcgccg acgatggtgt cataaccgct cgcgcgtccg
     5701 gcgatgaatt catgcgcatg ggcctggtcc gccgc
//
LOCUS       DASYEG010000021         5814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3341493, whole genome shotgun sequence.
ACCESSION   DASYEG010000021 DASYEG010000000
VERSION     DASYEG010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5814
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3341493"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..968)
                     /locus_tag="VGM72_00910"
     CDS             complement(81..968)
                     /locus_tag="VGM72_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY4263850.1"
                     /translation="MKLARFDDDRIGVVRGDEIVDVTDIADGRTGEWPPVAMTRLIAN
                     FAQHRDALERAGASGEGVPLARVKLLTPVPWPNKVIAYPVNYHEHGAEMGASYRANNQ
                     GFFLKPPSALSGPSDPIVLPVNAGRRIDHECELAIIIARQGRDIPRERWREYAFGYSC
                     LIDAVIRGPEERVTRKGFDTFCPVGPWIVTEDEIGDAVSNLQGRLWVNGALKQDANTR
                     DLVLDIPGMIEMASSVMTLYPGDIIATGTPAGVGPMRPGDIVKIAFERVGEMTLDVVQ
                     GRSGQRSIFAQAVPADAKT"
     gene            complement(1837..3321)
                     /locus_tag="VGM72_00915"
     CDS             complement(1837..3321)
                     /locus_tag="VGM72_00915"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY4263851.1"
                     /translation="MADSGTEAIASRSQHRVAHEDLRDLLRDFEAMREVQRIEGADPH
                     LEISALAETLTMRFPGEEPALLFDKIKGFPEGFRILSGAANSFRRLAHLFGLPSPEKK
                     IDIVKSYKAQLGKGPELIPPRILPTGPVMENVQRDGEVDIFKFPIPFVHEHDGGRYIG
                     TEDLVVMRDPDSDWVNVGTYRIVAHAKDEVGIWISPGKHGRLIREKYFARGKPCPVLI
                     SCGQDPILFLCSNAELPLGVSEFDYAGGQRGRAVDVVLSELHKLPMPAGAEIVLEGEM
                     YGDRLKMEGPFGEFMGYYASEASEQPIVKIRRVYHRDDPILTLAIPSRPPSNFTVARA
                     VVKAAMIWDEIEKAGLPGVQGVWNHEAGAGRLFNVVSIKQMYAGHAKQAGQLAANCRS
                     GVYAGRWVVVVDEDIDPSNIHDVIWAMSTRCDPAEDIELIHKLWSTPLDPILRGPPYQ
                     SNRAVIDACRPWGWKDEFPRVAEASPELKDAVMKKWPNLFRARS"
     gene            complement(3449..3826)
                     /locus_tag="VGM72_00920"
     CDS             complement(3449..3826)
                     /locus_tag="VGM72_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4263852.1"
                     /translation="MTQSPRASVLVVEDEALIAMDLQALLEDAGYRVLGPANSTASAM
                     AILAGQDPDIALLDVNLGRSDVFGVANELATRKTKLIFLTGHTAHKLPPAHQHRPLVA
                     KPYLPHVLLAAVELALSQADGAA"
     gene            complement(3847..5034)
                     /locus_tag="VGM72_00925"
     CDS             complement(3847..5034)
                     /locus_tag="VGM72_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HEY4263853.1"
                     /translation="MTAHAMPEPTPEGEPGESSKAQDAPKAKILIVDDDERTAMAVRT
                     TLEELGQYLVVAHSGEEALHRLLFDDYAVILLDVNMPGMDGYEMASFVRARKRTRHIP
                     IVFLTALFRDDSHLLQAYSAGAVDMVFKPVDPFILRSKVAVFVDLYLKQAEIQREAEL
                     RHRLQEENFRVRAEKLTAEQALRRSQEHQEAMAKAEAHQRTLIEELNHRVKNMLTVVN
                     AMARQTLASNPAPQDFTEKFVRRIDALGRTHSLLSREQWATVQLCEVARDSLEPYVLE
                     DKNRVVLDGPSVAFDPKTALALGIVFHELATNAIKHGALSNREGTVTMRWSFADDGKI
                     ALQWQEHGGPPCSPPARSGFGSRLIRLELTHELNGDVELIYEPGGLKVMMGFPLSNAL
                     AMA"
     gene            complement(5031..>5814)
                     /locus_tag="VGM72_00930"
     CDS             complement(5031..>5814)
                     /locus_tag="VGM72_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4263854.1"
                     /translation="NNLGDAQESILANGFDCVVLDLSAPSGISLGDLVKTLAASDEPV
                     VVFLPRDPNEEERAYLTQLARRSEPVRIASSTAQLQDHIALILHRPFENLPLNAQAAL
                     QQLRRCDPLLAGKKVIVIDDDIRNIFSLTSALEEHGVELHYAESGRSGIEILQRVPDA
                     DAVLVDIMMPGMDGYQTMQEIRTIPEFATLPVIAVTAKAMKGDRQKCIEAGASDYVAK
                     PVDIDQLVSVLRVWFQRAGQTAPAMGAAGDNVVTFRSVAGQN"
BASE COUNT         1136 a         1807 c         1732 g         1139 t
ORIGIN      
        1 tgggcgatca agcgcttttg atcgcgagaa acgactgggg ttttgcggta ggcgttttgc
       61 gaatgcttcc cgctcgcgcg tcaggtcttc gcgtccgccg ggaccgcctg ggcgaaaatc
      121 gagcgttggc cgctcctgcc ttgcaccacg tcgagggtca tctcgccgac gcgctcgaac
      181 gcgattttca caatgtcgcc gggccgcatc ggccccacac cggcgggcgt tcccgtcgcg
      241 atgatgtcgc cgggataaag cgtcatcacg gacgacgcca tctcgatcat ccccggaatg
      301 tcgagaacca gatcgcgcgt gttggcatcc tgcttgagcg cgccattgac ccatagccgg
      361 ccctgcagat tcgacaccgc atcgccgatt tcatcctcgg tgacgatcca tgggcccacc
      421 ggacaaaagg tgtcgaaacc cttgcgcgtc acgcgctcct cgggcccgcg gatcaccgca
      481 tcgatcaggc aagaatagcc gaaggcatat tcgcgccatc tttcacgcgg gatgtcccgc
      541 ccttggcggg cgatgatgat tgcgagttcg cattcatgat cgatgcgacg ccccgcattg
      601 acgggcagca cgatcgggtc ggacggcccg gacaacgccg agggcggttt taagaaaaat
      661 ccctgattgt tggcgcgata ggacgcgccc atttcggcgc cgtgctcgtg gtaattcacc
      721 ggatacgcga tgaccttgtt cggccacggc accggggtca gcagcttgac gcgcgcgagc
      781 gggacgccct cgccagacgc cccggcgcgt tcgagcgcat cgcgatgctg cgcgaaattc
      841 gctatcagcc gggtcatcgc gaccggcggc cactcgccgg tgcgcccatc ggcgatatcg
      901 gtcacatcga cgatttcatc gccacggacc acaccgatgc gatcgtcgtc aaagcgcgca
      961 agtttcatct ttagtctctt tcgctacggt actcgcctac ctgccagagc ggaaaaaaag
     1021 gcaaagctag gctatttgaa tagacgcaaa aatgtccggt gcagacgtaa cgcatgcagc
     1081 aagtcactcc ttcatcgact tcacgtgcta gcgtttagtt ctcagtcttg gtccgaccat
     1141 cggcgtcgcg cggtattaag taattcgaca agtttaggcg ctccgatctc ccatccgaac
     1201 cccaagacac cgtgacttcc tgaggtcgcc ttgttcactg ggcggaggaa gttcgcaact
     1261 gacctccgac caaaagtgtc gcaaccaaca aacccaccca ttgcgatgcg aaaattgcct
     1321 atctccctcc aagaccagtg gcgtgatccg aaggtgttgc gccacgtcag tccatctgag
     1381 ccgagaatta acttcatcgg gtcaatcaat tgccaactga agtagagcag cacgaggccc
     1441 cctgaatatc gaaaagcgac agacccatct gggcgggaaa gacttaagta caggatggca
     1501 gccgatgcta tgacgccaat gctcgatacg cgccgtgagg cgcgcaccac aatcggcgga
     1561 tcgactaagc tcccctgcat aacccctcgc gcttgagcag gacaatacga cgacactcaa
     1621 ccgtaccaag tgcgacagat agatggtgca gcgtcaacgt cgagttaagt ctgaaaaggg
     1681 tcgattctgt tgaaaaactc cgaaatgcag aacgtgctga attgcgataa catcgtcatt
     1741 cgcttaaagc cgctacgcat ctctatctgg agcactctgg gcggctctag ctggtcataa
     1801 gacggccccc cgcattcaat tttgaatatc gccaacctag cttctggccc gaaacaaatt
     1861 tggccatttc ttcatgaccg cgtccttcaa ttcggggctc gcttcggcga cgcgcgggaa
     1921 ttcatctttc catccccagg gccggcaggc atcgatcacc gcgcgattgc tctgataggg
     1981 ggggccgcgc aaaatcgggt ccagaggcgt gctccacaat ttgtggatca gctcgatgtc
     2041 ctcggccgga tcgcagcgcg tcgacatcgc ccagatgaca tcgtggatgt tggaggggtc
     2101 gatatcctca tcgaccacga ccacccagcg cccggcatag acccccgacc ggcaattggc
     2161 cgcgagctgg cctgcctgtt tggcgtggcc ggcatacatc tgcttgatgg agacgacatt
     2221 gaacagccgg cccgcgccgg cttcgtgatt ccacacgccc tgcacgccgg gaagcccggc
     2281 cttctcgatc tcgtcccaga tcatggcggc cttgaccacg gcgcgcgcca ccgtgaaatt
     2341 cgaaggcggc cgcgacggga tggcgagcgt cagaatcgga tcgtcgcgat gatagacgcg
     2401 acggatcttg acgatgggct gctcgctcgc ctcgcttgcg taatagccca tgaactcgcc
     2461 gaacggacct tccattttca ggcggtcgcc atacatctcg ccttcgagca cgatttccgc
     2521 acccgccggc atgggcaatt tgtggagttc gctcaacacg acatcgaccg cgcgcccgcg
     2581 ctggccgccc gcataatcga actccgacac gccgagagga agttccgcat tgctgcaaag
     2641 gaagagaatg ggatcctgac cgcaggagat cagcaccggg cacggcttgc cgcgcgcgaa
     2701 atatttctcg cggatcagac gcccgtgttt tccgggcgat atccatatgc caacctcgtc
     2761 cttcgcgtgc gcgacgatgc gataggttcc gacattgacc caatcgctgt ccggatcgcg
     2821 catcacgacc agatcttcag tgccgatata gcgtccgcca tcgtgctcat ggacgaaggg
     2881 gatggggaat ttgaagatgt ccacctcgcc gtcgcgctgc acattctcca tgacggggcc
     2941 ggtgggaagg atgcgcggcg ggatcagctc agggcccttg ccgagctgag ccttgtagct
     3001 cttcacgatg tcgattttct tctccgggct cggcaggccg aacagatgcg cgagccggcg
     3061 aaaggaattc gccgcccccg aaagaatgcg gaagccttcc gggaaacctt tgattttgtc
     3121 gaacagcagc gccggctcct cgccgggaaa gcgcatggtc agggtttcgg cgagcgcgct
     3181 gatttccaaa tgcgggtcgg cgccttcgat gcgctgaacc tcgcgcatgg cttcgaaatc
     3241 gcgcaggaga tcgcgcagat cttcgtgcgc gacgcggtgc tgggaccggc tcgcgattgc
     3301 ctctgtacct gaatcggcca tgaaccctcg tcttcccgca ggccaaagcc ccgatttgct
     3361 tggccttata tttcacttgg ggggcgcgcg aaagcatggg cccgcatttc agccaggcga
     3421 aggcgagact tcaggatttc atgaaaaact aggcggcgcc gtccgcttgc gaaagcgcca
     3481 attccacggc cgccagcagc acatgcggca aatagggttt ggccaccaaa ggacggtgct
     3541 ggtgcgcggg cggaagcttg tgtgccgtgt gaccggtgag gaagatcagt tttgtcttgc
     3601 gcgtcgccag ctcattggcg acgccgaaca cgtccgagcg cccaagattg acgtcgagga
     3661 gggcgatgtc gggatcctgg cccgccagga tcgccatggc ggaggccgtc gaattggccg
     3721 gtcccagcac ccgatagcct gcgtcttcca gcagggcctg caaatccatt gcaatcaacg
     3781 cctcgtcctc gacaacgagc acgcttgcac gtggagattg cgtcattggg ccaatccttg
     3841 tttcggctac gccatagcta gagcgttgga gagcgggaag cccatcatca ctttgagccc
     3901 gcccggttca taaatcaatt ccacatcgcc gttcagttca tgcgtcagtt ccagacggat
     3961 cagccggctg ccaaaaccgc tgcgcgccgg tggggagcac ggcggcccac catgctcctg
     4021 ccattgcagg gcgattttgc catcgtccgc gaaactccag cgcatcgtca cggtgccctc
     4081 gcgattggac agcgcgccgt gttttatcgc gttggttgcg agttcgtgaa acacgatgcc
     4141 cagcgcaagc gccgttttcg gatcgaacgc gaccgaagga ccatcgagca caaccctgtt
     4201 cttgtcctcc agcacatagg gttccaggct gtcgcgtgcg acctcgcata gctgcacggt
     4261 tgcccattgc tcgcgcgaca gaagactgtg cgtgcgtccg agcgcatcga tgcggcggac
     4321 gaatttttcc gtgaaatcct ggggcgcggg attgctggcg agcgtctggc gcgccatggc
     4381 gttgaccacc gtcagcatgt tcttgacgcg gtgattcagc tcttcgatca gggtgcgctg
     4441 atgggcctcg gccttcgcca tcgcctcctg atgttcctgg ctgcggcgaa gcgcctgctc
     4501 ggcggtgagc ttttcggcgc gcacacggaa attctcttcc tgaagccggt ggcgcagttc
     4561 cgcttcgcgc tggatttcgg cctgcttgag atagagatcg acgaacaccg cgactttgga
     4621 gcgcaggatg aacggatcga ccggcttgaa gaccatgtcc accgcgccgg ccgaataggc
     4681 ctggagcagg tgcgaatcgt cacggaaaag cgcggtcaga aacacgatcg ggatatggcg
     4741 tgtgcgcttt cttgcccgca cgaacgaagc catttcatag ccgtccatgc cgggcatgtt
     4801 cacatcgagc aggatgaccg cgtaatcgtc gaagagaagc cgatgcagcg cctcctcccc
     4861 cgaatgggcg acgaccagat actggccgag ttcctccagg gtggtgcgga ccgccatggc
     4921 ggtgcgctca tcgtcatcga cgatgagaat cttcgccttg ggcgcatcct gcgccttcga
     4981 cgattcgccg ggctcgcctt caggcgtcgg ttcgggcatc gcgtgcgcgg tcaattctgg
     5041 cctgccacgg agcggaacgt gacgacatta tcgcccgccg ctcccattgc aggcgccgtc
     5101 tggccggcgc gttggaacca cacccgcaac accgagacga gctggtcgat atccacgggc
     5161 ttggccacat aatcggatgc ccccgcttcg atgcatttct gacggtcccc tttcatcgcc
     5221 ttggcagtca ccgcgatgac cggcagcgtt gcgaattccg ggatggtgcg gatttcctgc
     5281 atggtctgat agccgtccat gccgggcatc atgatatcca ccagcaccgc atccgcgtcg
     5341 ggcacgcgct gcaggatttc gatgcccgag cgccccgatt cggcgtagtg caattcgacg
     5401 ccatgctcct ccagcgcgct cgtgagcgag aagatgttgc ggatgtcgtc gtcgatgacg
     5461 atcaccttct tgcccgccag cagcggatcg cagcggcgga gctgttgcag ggcggcctgg
     5521 gcattgagcg gcaggttctc gaatggccgg tgcaggatga gcgcgatgtg atcctgcaat
     5581 tgtgccgtgc tcgaggcgat gcgcaccggc tccgaacggc gcgcgagctg ggtgagatag
     5641 gcgcgctcct cctcgttggg atcgcgcggc aggaacacca cgaccggttc gtcagaagcc
     5701 gcgagggtct taacgagatc gcccagcgag atcccggacg gcgccgaaag atcgagcacg
     5761 acgcagtcga agccattggc gaggatcgac tcctgcgcat ctcccaaatt gttg
//
LOCUS       DASYEG010000022         6435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_298115, whole genome shotgun sequence.
ACCESSION   DASYEG010000022 DASYEG010000000
VERSION     DASYEG010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6435
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298115"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /gene="tssC"
                     /locus_tag="VGM72_00935"
     CDS             <1..658
                     /gene="tssC"
                     /locus_tag="VGM72_00935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HEY4263855.1"
                     /translation="CWMNAAYVLGTRITDSFSQYGWCTSIRGAEGGGKVQNLPTHTFV
                     SDDGDMDMKCPTEIAITDRREFELSQCGFFPLSHYKNTDYAVFFGAQTTQKPKKYDRP
                     NATANAAIAARLPYLLATSRFAHFLKVMARDKIGSFMEKDDCAAWLQRWISNYVNASD
                     TASAESKARFPLRDARIEVKEIPGKPGSYNAVAYLQPWLQMEELTTSLRLVAKIPQKA
                     "
     gene            683..2236
                     /gene="tssC"
                     /locus_tag="VGM72_00940"
     CDS             683..2236
                     /gene="tssC"
                     /locus_tag="VGM72_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HEY4263856.1"
                     /translation="MELDANVMPRQADLGPPPEAAENREDLRERLIRYLEGIAGVRDG
                     DAGLPLDQLATQEALAAWFGPSFTELYGKPDALRQALDRDVAAIDDFISNQVNAILHD
                     ASFQRLETAWRGVQFLTGIAAEISGAKIRVLPVSWNEIARDLDRAGDFDQSQIFQKIY
                     SNEFGTPGGEPFGLLIGDYAIHHQRTADHPTDDIGALKSLASVAAASFAPLVLGVSPS
                     VFQLNSFSQLGRPIELRTIFAQGEYQRWNAMRQNEDLRFAGLVLPRLLMRLPYVDDPL
                     RRDGFHFAEHTDSRDGSGYLWGNAAYAFGATVLRAFGNFGWFADIRGAPRDELRGGLV
                     YGNPIASFATDSPGIALKPSTECLISEHQERDLTELGFIPLRDIAFTEFSVFNESPSL
                     HMPERYDRQITTINARISAMLPYVLCVSRFAHFIKIMARERIGSFSTADDCQAFLQTW
                     LGRYCEGSSDASAETRARYPLREGTIQVKNVPGKPGTYSCTVLLRPQYQFDEVSAGFR
                     LVTELSPAN"
     gene            2260..3069
                     /locus_tag="VGM72_00945"
     CDS             2260..3069
                     /locus_tag="VGM72_00945"
                     /inference="COORDINATES: protein motif:HMM:NF018699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
                     /protein_id="HEY4263857.1"
                     /translation="MTTNAVQDHLKAGELDEAIGLMNAEIRANPKDTNRRAMLVELLC
                     FAGNLERADTVLDAISTVDPGAAVGVAMVRQLVRAEQARQQFYSEGRVPEFVKKPGGA
                     MELELRAAIALREGASGEAASLLAEAEAIRPRAPGTVDGVAFDDFRDLDDLSAAHFEV
                     LTATGKYYWVPVESITSLEFRPPGRRRDLLWRRAAMTVTEGPDGEVFIPAIYPFRGAA
                     ATPRYRLGYATDFLGEETGPKFGIGLRTFLVGDDSRTILELGKLEFVKPAA"
     gene            3140..3631
                     /gene="tssE"
                     /locus_tag="VGM72_00950"
     CDS             3140..3631
                     /gene="tssE"
                     /locus_tag="VGM72_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HEY4263858.1"
                     /translation="MINPDTPLLPSLLDRLMDDHPERQQDAPRSRAQNLAALQDSIRR
                     DLEALLNTRRRCVSPPPALEDLDTSLIEYGVPDFLSVNAASERVREELRAALETAIRL
                     FEPRFKTFSVKLVEDSDQIDRAFRFRIDALMYAEPAPEHVSFDSTLNPGNHRFSVVGR
                     RNG"
     gene            3624..5444
                     /gene="tssF"
                     /locus_tag="VGM72_00955"
     CDS             3624..5444
                     /gene="tssF"
                     /locus_tag="VGM72_00955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HEY4263859.1"
                     /translation="MADDLLSYYNRELAYLRRLGAEFAEAHPKIAGRLRITGDAVEDP
                     HISRLLEGVAFLNARTRRKLDDEFPELTDALLGQLFPHYLRPIPSMAIVQFRPASDQE
                     GGYRIAAGTEIETEPVGGEPSRYRTTQEQTLWPIEIETASLTGRPLQAPKNPHSGGAV
                     AVLRLSLRCMADEMTFSTLAPESLRVFLRGTSSEVFQLYELIFNHTVSIALADSTSDP
                     NPIILDVASLRPVGFAPEEGILPYPPRSPVGYRLLTEFFAFPEKFLFFEIENLSTKVL
                     MGAGRKLDIFLYLNDTAQELERNLSADSFALGCVPTVNLFAQRAEPIRLEETNAEYHI
                     VPDSRRPDALEIYSVDGVIATSQTGEATKFEPFFGLKHAGATRGRTRFWHSAQRPAGG
                     RDPASETYLTLVDLDSDPSIPAEWVASVETTCFNRNLPGKLPYGGGHPYLKLIRDTPA
                     VESLQCLTAPTPTLRLPSRNEGAWRLVSHLTLNHLSLADSEHGAEALREMLRLYDFRD
                     TPETRLLIDSLLSVRAVRGVARPPDAAMGALCRGLEVTIEFDDRRQSGAGIFLLAAIL
                     ERFIAHYASINAYTRLTATLKGRPGVYRKWPARAGDQQLL"
     gene            5510..>6435
                     /gene="tssG"
                     /locus_tag="VGM72_00960"
     CDS             5510..>6435
                     /gene="tssG"
                     /locus_tag="VGM72_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HEY4263860.1"
                     /translation="MAAIRSREDRSAAPARIGLDHDPRQESLFLRSAVERTFPATEVV
                     ALEGEDTRPELSVTLMGLNGASGVLPEFYSQFIMAELRHKNTALRDFLDIFNHRALSL
                     FARAMEKYRLPLAFERAGYENQDGITRALFALVGMAAPSLRHRQSVADSTLLFYAGHL
                     SRSRPTALGLSQILGDYFEQPVTVAQFQGRWVELPQSEQTVLGGRTNPGRYAQLGINA
                     VAGIRVWDVQGAFRVRLGPLDYREFSAFMPPGARMAELAALVRTYAGPALSFDVQLIL
                     KADEIPLLQLSRDEESGPRLGWNSWLPTERRKA"
BASE COUNT         1181 a         2028 c         1940 g         1286 t
ORIGIN      
        1 ttgctggatg aacgcggcct atgtgctggg cacccggatc acggattcct tctcgcaata
       61 tggctggtgc acttcgatcc gcggcgccga aggcggcggc aaggttcaaa atctgccgac
      121 ccacaccttt gtcagtgacg acggcgacat ggacatgaaa tgccccaccg aaatcgcgat
      181 caccgaccgg cgggaattcg agctttccca atgtggcttc ttcccgctgt cgcactacaa
      241 gaacaccgat tacgcggtct tcttcggcgc ccagaccacc cagaagccca aaaaatacga
      301 ccgtcccaac gccaccgcca atgcggctat cgccgcccgc ttgccctatc ttctggcgac
      361 ctcgcgtttt gcgcatttcc tgaaggtcat ggcgcgcgac aagatcggca gcttcatgga
      421 gaaagacgat tgcgccgcct ggctgcagcg ctggatcagc aattatgtga atgccagcga
      481 tacggccagc gcggaatcca aagcccgctt tccgctccgc gacgcacgca tcgaggtgaa
      541 ggaaatcccc ggcaagccgg gctcctacaa cgccgtcgcc tatttgcagc cttggctcca
      601 gatggaggag ttgacgacct ctctccggct ggtcgccaag attccgcaga aggcttaagg
      661 agcggatttg ggaggaggcc ccttggagct tgacgccaac gtcatgccgc gtcaggccga
      721 tttgggtcct cccccggagg ccgccgagaa tcgtgaagac ctgcgtgaac ggcttatccg
      781 gtatctggaa ggcatcgccg gcgttcgtga cggcgatgcc ggtttgcctc tcgaccaatt
      841 ggccacgcag gaggcactgg cggcgtggtt tgggccgtct ttcaccgagc tgtacggaaa
      901 accggacgcg ctgaggcagg cgctggaccg cgatgtcgcc gcgatcgatg atttcatttc
      961 caaccaggtc aacgcgatcc tccatgacgc gagctttcaa cgattggaga cggcatggcg
     1021 cggcgttcaa ttcctcaccg gcatcgcggc cgagatcagc ggcgcgaaga ttcgcgttct
     1081 tccggtgagc tggaatgaaa ttgcgcgcga cctcgaccgc gccggcgatt tcgatcagag
     1141 ccagatcttc cagaaaatct attccaatga attcggcacg cccggcggcg aacccttcgg
     1201 cctcttgatc ggcgattatg cgatccatca tcagcgcacg gccgatcatc ccaccgacga
     1261 tatcggtgcg ctcaaatcgc tggcatcggt ggcggctgct tcgttcgccc cccttgtcct
     1321 tggcgtttcg ccttccgtat tccagctcaa tagcttcagc caactgggca ggcccatcga
     1381 gcttcgaacc attttcgcgc aaggtgaata tcagcgctgg aacgcgatgc gtcagaacga
     1441 ggatttgcgc ttcgccggtc ttgtgctgcc ccgccttttg atgcgcctgc cctatgtgga
     1501 cgatccgttg cgccgcgacg gctttcactt tgccgaacat accgatagcc gcgacggcag
     1561 cggctatctc tggggcaatg cggcctatgc ctttggcgcc acggttcttc gggccttcgg
     1621 caatttcggc tggttcgcgg acatccgcgg cgcgccgcgc gacgagctga gaggcggcct
     1681 ggtctatggc aatcccatcg cgtcttttgc gaccgacagc cccggaatcg cgctgaagcc
     1741 ttctaccgaa tgtctgatct cggagcatca ggagcgggac ctgacggaac tcggattcat
     1801 tccgttgcgc gacatcgcgt tcacggaatt ttcggtgttc aacgaaagcc cttccttgca
     1861 catgcccgag cgttacgacc gtcagatcac caccatcaat gcccggattt ccgcgatgct
     1921 gccctatgtg ctctgcgtgt cgcgctttgc ccatttcatc aagatcatgg cgcgcgaacg
     1981 gatcggctct ttcagcacgg cggacgattg ccaggcattt ctccagacct ggttgggccg
     2041 ctattgtgaa ggaagcagcg atgcctcggc cgaaacccgg gcgcgctacc cgttgcggga
     2101 ggggaccatc caggtgaaga acgtacccgg caaacccggc acctattcgt gtacggtgct
     2161 gctgcggccg caatatcagt tcgacgaagt ctctgccggc ttcagattgg tcacggaatt
     2221 gtcgcccgcg aattgaggcg ggccaagggg acgggtgcga tgacgaccaa tgccgtgcag
     2281 gatcacctca aagcgggcga actcgacgaa gccatcggtt tgatgaatgc ggaaatccgc
     2341 gcgaacccga aggacacgaa ccgccgggcc atgttggtgg agctgctgtg ctttgcgggg
     2401 aatctggagc gcgccgacac cgtcctggac gcgatcagca ccgtcgaccc gggtgcggcg
     2461 gtcggcgtcg cgatggtccg ccaactcgtc cgcgccgagc aggcccggca gcaattctac
     2521 tccgagggca gggtcccgga gttcgtgaag aaaccgggcg gcgccatgga gcttgaattg
     2581 cgggccgcca ttgcgctgcg cgaaggcgca tccggggaag cggcgtcgct gttggccgag
     2641 gccgaagcga tccgtccgcg cgcgccggga accgtggatg gcgtggcctt cgatgatttc
     2701 cgcgatctcg atgatttgtc cgcggcgcat ttcgaggtgc tgaccgcgac cggaaaatat
     2761 tattgggtac cggtggaaag tatcacctcg ctcgaatttc gccctcccgg gcgccggcgc
     2821 gatttgctct ggcggcgcgc cgccatgacc gtgaccgaag gcccggacgg agaggtcttc
     2881 ataccggcga tctatccctt ccgaggagcg gccgcgacac cgcggtatcg cctcggctat
     2941 gcgaccgatt tcctgggcga agaaaccggt ccgaaattcg gaattgggct tcgcactttc
     3001 ctcgttggcg acgacagccg cacgatcctg gaattgggca aactcgaatt cgtgaaaccg
     3061 gcggcctgac ttcgatgacc gcattgtcca gaccgcatga tcgcgcaacg tccctgggac
     3121 aaaagccagg ggcgatgcga tgatcaatcc cgacacgccg ctcctgccgt cgttgctcga
     3181 ccggctgatg gacgaccatc ccgagcggca gcaggatgcg cccagaagcc gggcgcaaaa
     3241 tctggcggcc ctgcaggatt cgatccggcg cgatctcgag gcgctcctca acacccggcg
     3301 gcgctgtgtt tctccgcctc ctgcgctgga ggatctcgac acatcgctga tcgaatatgg
     3361 ggtgccggat tttctttccg tgaacgccgc ctcggaaagg gtgcgggaag aattgcgcgc
     3421 cgcactcgaa accgccatcc ggctcttcga accacggttt aagacctttt cggtcaagct
     3481 ggtcgaggat tcggatcaga tcgaccgcgc cttccgcttt cgcatcgacg cgctgatgta
     3541 cgccgagccg gcgcccgaac atgtcagttt cgattcgacg ttgaatccgg gcaatcacag
     3601 gttttcggtc gtaggccggc gcaatggctg acgatttgct gtcctattac aatcgcgaat
     3661 tggcgtatct gcgccggctg ggcgcggaat tcgccgaggc gcatcccaag atcgctggcc
     3721 gcctgcggat tacgggcgat gcggtggagg atccgcatat ctcgcgtctt ctcgaaggag
     3781 tggccttcct caacgcccgc acacgccgaa agctggatga cgagtttccg gaactcactg
     3841 atgcgctgtt gggacagctc tttccccatt atctgcggcc gattccctcc atggccattg
     3901 ttcaattccg ccccgcaagc gatcaggagg gcggctaccg cattgcggcg ggcacggaaa
     3961 tcgagaccga gccggtgggc ggcgaaccca gccgctatcg cacgacccag gaacaaacgc
     4021 tgtggccgat tgaaatcgaa acggcgtcgc tgacaggccg tccgcttcag gcaccgaaaa
     4081 atccccattc cgggggggcc gttgcggttc tgagattgtc gctgcgctgc atggccgatg
     4141 agatgacgtt ctcgaccctg gcgccggaat cgctgcgcgt gtttctgcgc ggcacgtcct
     4201 ccgaggtgtt ccagctttac gaactgatct tcaatcacac ggtttccatc gcgctcgcgg
     4261 attcgacctc ggaccccaac ccgatcattt tggatgttgc gtccctgcgc ccggtggggt
     4321 tcgcgcccga agagggtatc ttgccctacc cgccgcgctc cccggtcggc taccggctgc
     4381 tgacggagtt cttcgcgttc cccgagaaat ttctgttttt cgaaatcgag aacctttcca
     4441 ccaaggtgct catgggcgcc gggcgcaagc tcgatatatt tctctacctc aacgacaccg
     4501 ctcaggaact ggagcgcaat ctttcggcgg attcattcgc gcttggctgc gtgcccaccg
     4561 tcaatctgtt tgcgcagcgc gccgaaccta tccggcttga agaaaccaat gccgaatatc
     4621 acatcgtgcc cgacagccga cgccccgacg cgctcgaaat ctattcggtg gacggagtca
     4681 tcgcgacgtc gcagaccggg gaagcgacaa aattcgagcc cttttttggt ttgaagcacg
     4741 ccggcgcaac acggggacgg acccggttct ggcatagcgc acagcgcccg gccggaggcc
     4801 gcgatcccgc ttcggaaacc tatctcactt tggtggattt ggattcggat ccttcgatac
     4861 cggccgagtg ggtggcctct gtcgagacga cttgcttcaa ccgcaatctg cccggcaagc
     4921 tgccttacgg cggcgggcat ccctacctca agctcattcg ggacacaccg gcggtcgaga
     4981 gtttgcaatg ccttaccgcc ccgacgccga cattgcgtct gccaagccgg aacgagggcg
     5041 cctggcggct cgtgtcgcat ctgacgctca accacctctc gctggccgac agcgaacacg
     5101 gcgccgaggc tttgcgcgaa atgctgcgtt tgtatgattt ccgcgatacg ccggaaaccc
     5161 gcttgctgat cgacagcctc ctcagcgtgc gcgccgtgag gggcgtagcg cggccgccgg
     5221 atgccgccat gggcgcgctc tgccgcggtt tggaggtcac aatcgaattc gatgacagac
     5281 gccaatcggg cgcgggcatc tttctgctcg ccgccatatt ggaacgtttc atcgcccact
     5341 acgcatccat caacgcgtat acgcggttga ccgcgacgct caaaggacgc ccaggagtct
     5401 atcgaaaatg gccggcgcgc gcaggcgacc agcagcttct ttgatcgagc gcctgcggtc
     5461 gtcgccgcag gcgtttgctt ttctgcaggc tgttcgcatt gtcgaacgca tggccgcaat
     5521 ccggtcgcgc gaggaccgct ccgctgcgcc tgcgcggatc gggctcgatc acgatccgcg
     5581 ccaggagagc ctgtttctcc gcagcgcggt ggagcggaca tttccggcaa cggaagtcgt
     5641 cgccctcgaa ggcgaggaca cgcgtcccga actcagcgtc acgctgatgg gcctcaacgg
     5701 agcgagtggg gtcctgcccg aattctattc ccaattcatc atggcggagc tgcggcacaa
     5761 gaacaccgct ctgcgggact ttctcgatat tttcaatcat cgcgcgctct cgctgtttgc
     5821 gcgcgcgatg gagaaatatc gcttgccgct ggcattcgag cgggccggct acgaaaatca
     5881 ggacggcatc acacgcgctc tttttgcgct ggttggaatg gcggccccat cccttcgcca
     5941 tcgtcaatcc gtggccgatt cgacgttgct tttctatgcc gggcacttat cgcgttcgcg
     6001 accgaccgct ctcggcctaa gccagattct cggcgattat ttcgagcagc cggttacggt
     6061 cgcccaattc cagggccgct gggtcgaact gccgcagagc gagcaaaccg tcctcggcgg
     6121 acgcacaaat cccggccgct atgcgcaact cgggatcaac gcggttgcag gcatcagagt
     6181 ctgggatgtg caaggtgcct ttcgggtacg gctcggaccg ctcgactatc gcgaattttc
     6241 ggccttcatg ccgccaggtg cgcgaatggc ggagcttgcc gcgttggtgc gcacctatgc
     6301 cgggccggcg ttgagcttcg atgtccaact gatcttgaag gcggatgaaa tcccgctgtt
     6361 gcaattgtcg cgagacgagg aaagcggccc gcgtttgggt tggaacagtt ggctgccaac
     6421 cgaacgccgc aaagc
//
LOCUS       DASYEG010000023        14345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3778294, whole genome shotgun sequence.
ACCESSION   DASYEG010000023 DASYEG010000000
VERSION     DASYEG010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 14345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14345
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3778294"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VGM72_00965"
     CDS             complement(<1..272)
                     /locus_tag="VGM72_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY4263861.1"
                     /translation="MAVERTLSIIKPDATRRNLTGEIVARFEKAGLRVVAQKRMRLGK
                     AQAEAFYAVHSARPFFNGLVAFMTSGPVVVQVLEGEGAVLKNREVMG"
     gene            356..1369
                     /locus_tag="VGM72_00970"
     CDS             356..1369
                     /locus_tag="VGM72_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4263862.1"
                     /translation="MTGGRKHPGPAMPQLNLSVAIGDYDRNRPLIDGSVRIDGVDPVF
                     MKLVPEEIFFRAFRSQDFDICELSLSSFTLKTALGQCPYVGIPAFVSRCFRHTSIFVR
                     TDRIKKPEDLRGKRVGVPEWQLTANVWARAILEDDFGVKPSDMQWVRGGISDAARPEK
                     VAVNLPPGVQLEDAPEGTTISELLLKGEIDGFIAPRAPAVTSPNIGWLFPDPMAAGKD
                     YFQHTGIFPIMHLIGVRRTLAEKHPWLPFAVFKAFNQSKNIALEALTDVAATKVTLPF
                     VEEQLTAARALMGEDFWPYGVEHNRKALEAFLRHHHSQGISPRVLKVEELFHPATLES
                     FKI"
     gene            1396..3273
                     /locus_tag="VGM72_00975"
     CDS             1396..3273
                     /locus_tag="VGM72_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY4263863.1"
                     /translation="MLIIDNVTVRIAGRIILDGASAAISDGRKVGLIGRNGAGKTTLL
                     KLILGLHESETGKIELSRSWRVGAVAQEAPAGPLSLIETVLAADTERTRLLAAVDDLD
                     DPLKQGEIHARLDEIGAYAAPARAARILAGLGFPESQQRRPCSEFSGGWRMRVALGAI
                     LFAAPDLLLLDEPTNYLDLEGALWLEDYLKRYRGTIVLVSHDRDLLNRAVDSILHLEY
                     GRLTLYSGGYDRFVEAREQKRALDAAARAHQEAERKRIQAFIDRFRAKASKASQAQSR
                     MKWLEKMKPVTLVVADRLAAITLPQPEAVAPPLIALDRVSVGYAPGKPVLSHLSLRVD
                     SDDRIALLGQNGNGKSTLAKLMAGRLKPFAGEFTRARKLITGYFAQHQLDELDAEKTA
                     LATLWAHRPLFDETQARTRLGGFGFSGDKADTPIASLSGGERARLLLAIATLEKPNLL
                     ILDEPTNHLDIDAREELLTALNEYEGAVVLISHDRRLIEACADRLLLVADGRVTPYDG
                     DLEDYKRLVLSARGGTNDAVARDAKSQSKAEQRREAAGRRLTLKPLKDAMDKWEREVA
                     RLHGEIEKCDAGLAAPGLFATDPAKGEKLSKARAEAARKLEAAETHWIEAAEAYEAAD
                     V"
     gene            complement(3275..4516)
                     /locus_tag="VGM72_00980"
     CDS             complement(3275..4516)
                     /locus_tag="VGM72_00980"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY4263864.1"
                     /translation="MQAATAFGKRKPVAVAPPLAPKPVVSPATDVKGPAVPAVAARKM
                     DWPFLTLGLIGVLGLVYWAELRLGFDRGKGPAPGIGTLTALGGLDGMLVFQSGEWWRV
                     FTAPLLHGSVSHYVGNAIALLFAGMILERLIGAPWFAALFVVSGLGGAAGSLALNPGK
                     VISVGASGAIVGLLAAAFVCSFTFESVQLRRRMRKVSLRFRVPSLLPALLPLSTASSG
                     APVDYGAHIGGAVAGALMGFALSEIWPEASPRPALERLGSGIAMAGALAAAAAFALIS
                     VHYSEYRSYYTTHGSVLIPQSELPRNEHDGVARSLDLVDRYPRDPRGHLFRALYFLDR
                     RNASDAERQLRLGLAEHEVLMTDLPAQVEKSLQLALAVVLVIERRKAEAKEIAAPVCE
                     GAIPMEYVKLRKLLKDQGVCS"
     gene            complement(4638..5102)
                     /locus_tag="VGM72_00985"
     CDS             complement(4638..5102)
                     /locus_tag="VGM72_00985"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004347.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEY4263865.1"
                     /translation="MSETLFYHLERRTLEDVLPGLLERTRERDWRALVRVGSAERMEA
                     LDAHLWTYSEQTFLAHGTAAEGHKSRQPIYLTTEEENPNLAQVLFLVGGAMPPDWGAP
                     SLAQFVRIVLMFDGRDPEALTAARAAWRGAKDAGHAVTYWKESPSGKWEKQG"
     gene            complement(5121..6626)
                     /locus_tag="VGM72_00990"
     CDS             complement(5121..6626)
                     /locus_tag="VGM72_00990"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002077.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEY4263866.1"
                     /translation="MEISFAAPDAVSPGALVVGVLEGGVLSDAAMRADRATGGAVKRA
                     LQVSRFKGQAGHSLELLAPAGVKSSRIVLAGLGKADGFNEAVVERLAAAILARLLTSG
                     EERLTFAIDRPKNAKIAEADLAAHLAFGAHLRSYRFDHYRKVSDDEKPTLKKVVVLTP
                     EPGASRKAWNNLAAVGEGVFLARDLVNEPANILYPDEFAARTMALKKLGVKVEVLDVA
                     AMKKLGMNALLGVGQGSAHDSRMVVMQWNGAHKSGAGKDKAPLAFIGKGVCFDSGGLS
                     LKTAGGMMGMKGDMGGAAAVVGLMHALAKRKAHVNAVGLIGLVENMPDGKAQRPNDIV
                     KTMSGQTIEILNTDAEGRLVLADVLWYAQSRFKPRFMVNLATLTGAILVALGSEHAGL
                     FSNNDELSERLSKAGAAEGEPVWRLPLGDGYDRLVKSKIADMKNIGGPHAGSITAAQI
                     LQRFVNKVPWAHLDIAGVAWQDGEQKPLAPSWGTGWGVRVLNRLVADNYET"
     gene            6741..7874
                     /gene="lptF"
                     /locus_tag="VGM72_00995"
     CDS             6741..7874
                     /gene="lptF"
                     /locus_tag="VGM72_00995"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEY4263867.1"
                     /translation="MRKLFFYILRQIVGPFLLFALILTLVVWMTQSLRLLDLVINRGQ
                     SAAIFAYLTMLMLPSLLVVIAPIAFFGAALYALNRLNNDSELVVMWSSGVSRLQLALP
                     VLAAAMLVMTVCYACSLYLMPLGQRTMKDKVFDIRADIGAAILREGAFTTPSDGLTVF
                     IRELAPDGEIRGILVHDNREPKRPITYLAESGVLAQTQEGARLIMENGNIEQGEGGGA
                     RLSVLKFDRYVFDLDQYAGPQRASDRDTSERYLSELLDPPFPASQTVRRGVYIAEAHN
                     RLSGPLYCIAFALIALAATAKGRMGRTSYALRLAGAALIGAALRLIGYGAQGIAASNP
                     QFTLILYLLPLAASVAAAGVLTDIPLIPESIRRLIRAPVEEPA"
     gene            7871..8962
                     /gene="lptG"
                     /locus_tag="VGM72_01000"
     CDS             7871..8962
                     /gene="lptG"
                     /locus_tag="VGM72_01000"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEY4263868.1"
                     /translation="MTWSVTLMVYLAKRFLVAVLIVFAAFSALALSIDLADLFSRTTE
                     RGIPPEMVISMSLLKLPDIAQKLLPFAVLLGAVFVFSRMSRSHELVATRAAGISAWQF
                     LTPPLLVAVALGVVTMTAFNPMASAFLAQYTRLEARYIHGQASQLAVSSTGLWLRQGD
                     AAHQSVVHALRVADQGVHLEEVIVFLYQGLDHYAGRIDAASAELRNGDWHLTNAWVSG
                     ADGVPQHHDVYDLPTELTPSQIQESFASPDTISFWELPRFIANAEGAGFSALRHRLYW
                     YSLLALPLLFSAMVFMAASFSLRLARLGGTTQLVLAAVLSGFGVYFLGDVTGALGQSG
                     ILPTPLAALAPAAAASLLGMTLLFHEEDG"
     gene            8959..11151
                     /gene="lptD"
                     /locus_tag="VGM72_01005"
     CDS             8959..11151
                     /gene="lptD"
                     /locus_tag="VGM72_01005"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEY4263869.1"
                     /translation="MKRKGGKKPGIFRKARSVVTLAVAGALGSAMGPSAAEAQTSESS
                     RVLLQGDTVIYDSNTGIVTAEGHVEISDDERTLLADKVTYDENSNKVTASGNVSLQDS
                     TGNVAYADSVELTQDLREGALQGFAALIGKTGRLAASSGERREGRFTIAHGAVFTPCS
                     ICREDGERMPLWEIRAARIVHDQLQKEIYFEDATFEFLGLPVLYMPFFSEPDPTIKYK
                     SGFLLPDIGSIGAIGSFIKIPYYVSLSPSRDLTIDPFITTQGGTVLQTEYRERWGAGG
                     LWLQGTLGYDSEADGKPGESIWMGSLFGSGRVPLNDDGWRAGFDVQLASNKTYLKRYE
                     LSNLDRLTSDLFTDDVEGRSRAAITGYFFQSMRATDVTAQMPLALPLLEYTYIPEYKV
                     WGGRLQADSSALYLDRNQGTDMLRGSADVDWRRPFTTNDGELITFETFLRGDLYYIND
                     ATFDVPGAPRNSETIGRALGYGMIEWRWPFVADTNIPNTTLVVEPIAQVIAASGGGNP
                     PGLPNEDSTAFEFDSSNLFSANPAPGLDLWTGGPRSNVGFRASAILPNGSVEAMLGQQ
                     FRFSTDSSLPVGLRLNDDKSDIVGQFKIQFPNISLTHQFNIDPDDGTVRRNEVYLLAH
                     FGRSMINLSYLKLPQSAADPSLGEQEQINLTTTVQVYGNWGIFGEARRDLAKNQELES
                     SIGITYDDDCFVASLGFHRRDTATLNLKPSSAVIFRLGLKTGYTNRAP"
     gene            11347..12879
                     /locus_tag="VGM72_01010"
     CDS             11347..12879
                     /locus_tag="VGM72_01010"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY4263870.1"
                     /translation="MAAAAQDAAIPLQPGFLSQVPGAPVNPTGPEAEAPVIEVPSAEP
                     APATQPPAATTPPSDAATPPAEAMPAIASPPATASAVPANGVEPNAGKPGERVNIIGP
                     ANSTGVAAIVNDFVISNYDLDQRVALFVTTSGVRPTRDNLPQIRAQVLRSLEDEVLEL
                     QEAKKHKLTIAKTEVDKALQNIAEDNKLTVDQILKTIGQAGVTPTTFRQQVTAQLTWQ
                     KLVAARYGADVTVSDSQVDEAMTRLKQGSDKPQFLVAEIYVAVDRPEDEPAVRASVEQ
                     IAQQIKQGAPFQTVAGQFSQSPSAADGGDIGWVVEGQLAEELDHALAELQPGETTAPI
                     RAEGGYYVLQLRDRREPIGTVVKPAAATASDPDAPVPLDRLLIPLPQNPEPQLKERAL
                     TLAANVKAQVRSCAELPNVANQLQGSVYTRLGNMNPKDLDPALRDAVAKTSPGEGVAP
                     FFSAAGLELIFRCDAAPPKLVAFELPTREQLQQQLFVQQMGIYAKSYLRDLRRDAVVE
                     TR"
     gene            12863..13855
                     /gene="pdxA"
                     /locus_tag="VGM72_01015"
     CDS             12863..13855
                     /gene="pdxA"
                     /locus_tag="VGM72_01015"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF003699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY4263871.1"
                     /translation="MSKHDEGSGKLPIVISQGEPAGIGPEIAVKAWFMLGGHAGEHEL
                     RLIGSADILSRAAVSCGIDPRPLAHHIIDTGHGVNVQSGRPSAGNARAVTAAIERGVN
                     LCQSNEAAALVTAPIHKAVLTEAGFGFPGHTEFLAALTGRPRAIMMLASSETDPPLRV
                     VPFTIHIPLKDIFAKLDTDAIAMTAEIVIDALSADFGCPNPRLAIAGLNPHAGEDGTM
                     GAEDSDIIAPAISRLRARGHTVLGPLPADTMFHEQARRSYDAALCMYHDQALIPIKTL
                     AFWTGVNVTLGLPIVRTSPDHGTALDIAGKGIANPASMVAAIRMAADIAGRRGL"
     gene            13852..>14345
                     /locus_tag="VGM72_01020"
     CDS             13852..>14345
                     /locus_tag="VGM72_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HEY4263872.1"
                     /translation="MNQDRISEDGLPPLREVIAAHGLTPKRSLGQNFILDLNLTRRIA
                     REAEIAGAVVYEIGPGPGGLTRALLAEGAVRVIAVERDGRALPALAEIAAAKPGRLDV
                     VEADAMAIDERMLLSGIGVALPVPAVANLPFNVGTALLVKWLSADWPPWWQSLTLMFQ
                     KEVA"
BASE COUNT         2719 a         4584 c         4370 g         2672 t
ORIGIN      
        1 cccatcactt cgcgattctt gaggaccgcg ccctcgcctt ccagcacctg aacgaccacc
       61 ggacccgagg tcatgaacgc gaccaggccg ttgaagaacg gacgcgcgct gtggaccgca
      121 tagaacgcct cggcctgcgc cttgcccagc cgcatccgct tctgggccac cacccgcaag
      181 cccgctttct cgaaccgagc gacgatctcg cctgtcaagt tacgccgggt tgcatccggc
      241 ttgatgatgg aaagcgtgcg ctcgacggcc atgacccact cctgatgaaa gcgcgcgcct
      301 tatagcggcg ggttcgcaag gaccgcaagc tttgcgtcgc gggaggcttt tgcttatgac
      361 cggcggacga aagcatccgg ggcccgccat gccgcagctc aatctttccg tcgccatcgg
      421 cgattacgat cgcaatcgcc ccctgatcga cggctcggtc cggatcgatg gcgtcgatcc
      481 cgtattcatg aagctggtgc cggaggagat tttcttccgt gccttccgca gccaggattt
      541 cgacatttgc gagctgtcgc tgtccagctt cacgctgaaa accgcgctcg gccaatgccc
      601 ctatgtcgga atccctgcct ttgtctcgcg ctgtttccgc cacacctcca tcttcgtgcg
      661 caccgaccgg atcaagaagc cagaagattt gaggggcaaa cgcgtcggcg tgccggaatg
      721 gcagctcacc gccaatgtct gggcgcgcgc gatcctggaa gacgatttcg gggtgaagcc
      781 ttcggatatg caatgggtgc gcgggggaat ctccgatgcg gcgcggcccg agaaggtcgc
      841 ggtcaatctg ccgccgggcg tgcaactcga agacgcgccc gagggcacga ccatttccga
      901 acttctgctg aaaggcgaga tcgatggctt tattgcgccg cgcgcccctg cggtgacttc
      961 gcccaatatc ggctggctgt ttcccgatcc gatggcggcg ggcaaggatt atttccagca
     1021 caccgggatt ttcccgatca tgcacctcat cggcgtacgg cgaacgctgg ccgagaaaca
     1081 tccctggctg ccgtttgcag tgtttaaagc cttcaaccaa tcgaagaata tcgcgctgga
     1141 ggcgctcacc gacgtcgccg cgactaaagt gacgctgccc ttcgtcgagg aacaattgac
     1201 ggccgcccgt gctttgatgg gcgaggattt ctggccctac ggcgtcgaac acaatcgcaa
     1261 ggccctggaa gcctttctca ggcatcatca cagccagggc atttctccgc gcgtgctgaa
     1321 ggtggaagaa ttgttccatc cggccacgct cgagagcttc aagatctaac gccccgctag
     1381 ctcccgcgcg cgcccatgct gatcatcgac aatgtcacgg tacgcatcgc cgggcgcatc
     1441 atcctggacg gcgcgagcgc tgcgatttcc gacggacgca aagtcgggct gatcggccgc
     1501 aacggcgccg gaaaaacgac gctgctgaaa ctgatcctgg gcctgcatga aagcgaaacg
     1561 ggtaaaatcg agctgagcag aagctggcgc gtcggcgcgg tggcgcagga agctcccgcc
     1621 ggcccgctca gtctgatcga aacggtgctg gcagccgata ccgaacgcac ccggttgctc
     1681 gccgcggtgg acgatctgga cgacccgcta aagcagggtg aaatccacgc ccggctggat
     1741 gagatcggcg cctatgcggc gccggcgcgt gccgcgcgga ttcttgccgg cctcggattt
     1801 cccgaaagcc aacagcgccg gccctgttcg gaattctccg gcggctggcg catgcgcgtg
     1861 gcgctgggcg cgatcctgtt tgcggctccc gatctgctgc tgctggacga gccgaccaat
     1921 tatctcgacc tcgaaggcgc gctctggctg gaggattacc taaagcgcta tcgcggcaca
     1981 attgtgctcg tcagccatga tcgcgatctt ctcaatcgcg cggtggattc cattctccat
     2041 ctcgaatacg gcaggctgac gctttacagc ggcggttacg accgcttcgt cgaggcgcgc
     2101 gaacaaaaac gtgctctcga tgccgcggcg cgcgcccatc aggaggccga acgcaaacgc
     2161 atccaggctt tcatcgatcg tttccgcgcc aaggcctcca aagcaagcca ggcccagagc
     2221 cgtatgaaat ggctggaaaa gatgaagccg gtgacgctgg ttgtggccga ccgtctcgca
     2281 gccatcacgc ttccccagcc cgaagccgtg gcgccgcccc tgattgcgct ggatcgggtg
     2341 tcggtgggtt atgcgcccgg caagccggtg ctgtcccatc tttcgctccg cgtcgattcc
     2401 gatgaccgca tcgcgctcct cgggcagaac ggcaacggca aatcgacgct cgccaagctg
     2461 atggcaggcc ggctgaagcc gtttgcgggg gagttcacgc gcgcgcgcaa attgatcacc
     2521 ggctatttcg cgcagcatca gctcgatgaa ctggatgcgg agaagaccgc gcttgcaaca
     2581 ctctgggcgc atcggccgct gttcgacgag acccaggccc gcacgcggct cggcggcttc
     2641 ggcttttccg gagacaaggc ggacaccccg attgcctcgc tgtcgggcgg cgaacgcgcg
     2701 cggctcctgt tggcgattgc gacgctggaa aagcccaacc tcttgatcct ggatgagccc
     2761 accaaccatc tcgacatcga tgcgcgcgag gaattgctga cggcactcaa cgaatatgaa
     2821 ggcgcggtcg tgctgatcag tcatgaccgc cggctgatcg aggcctgcgc ggatcgcctt
     2881 ctgctcgtgg ccgatggccg cgtaacgcct tatgacggcg atctcgagga ttacaaacgc
     2941 ctggtcctgt cggcccgggg cggtacaaac gacgcggtgg cacgcgatgc caaatcgcaa
     3001 tccaaggccg agcagcgccg cgaagccgcc ggcagacgcc tcacgctgaa gccgctcaaa
     3061 gacgccatgg acaaatggga gcgcgaagtc gcgcggctgc atggcgagat cgagaaatgc
     3121 gacgctgggc tcgctgcgcc cggactgttc gcaaccgatc ccgccaaggg tgagaaattg
     3181 tcgaaggccc gcgccgaagc cgcgcgaaaa ttggaggccg ccgagaccca ttggatcgaa
     3241 gccgccgagg cctacgaagc ggcggatgtt tagattagct gcagacgccc tgatctttga
     3301 gcagcttgcg cagtttgaca tactccattg ggatggcgcc ctcacagaca ggggctgcga
     3361 tctccttggc ttccgctttt cgccgctcga tgacgagaac caccgcgagc gcgagttgca
     3421 gcgatttctc gacctgcgcg ggaagatcgg tcatgagcac ttcgtgctcg gcaagcccga
     3481 ggcgaagttg acgctcagcg tcggaggcgt tgcgtcgatc cagaaaatac agcgcgcgga
     3541 aaagatgccc gcgcggatcg cgcggatagc ggtccacaag gtccagcgag cgcgccacgc
     3601 cgtcatgctc gtttcgggga agctcgcttt ggggaataag aacggagcca tgcgttgtgt
     3661 aataggaccg atattcggaa taatgcacgc tgatcagcgc gaaggccgcg gcggccgcca
     3721 acgcaccggc catcgcgatg cctgatccaa gccgctcaag ggccgggcgc ggcgatgctt
     3781 ccggccagat ttcgcttaag gcaaagccca tgagagcgcc cgcgaccgcg cccccgatat
     3841 gggcgccgta atcgactggc gcgccggagg atgccgtact cagcggaagc aacgcgggca
     3901 aaagcgaggg gacacgaaag cgaagcgaga ccttgcgcat ccgccggcgc aattgcaccg
     3961 attcgaatgt gaagctgcag acgaacgctg cggccaagag gcccacgatg gccccggacg
     4021 cgcccaccga tatgaccttg cccggattga gtgcgagcga tccggccgcg ccgccaagtc
     4081 ctgagaccac aaacaaagcg gcgaaccagg gcgcaccgat caatcgctcc aagatcatgc
     4141 ctgcgaacag cagcgcgatg gcgttgccca cataatggga aacgctgccg tgcaggagcg
     4201 gtgctgtgaa cacgcgccac cattcgccgg attggaagac cagcatgcca tccagcccgc
     4261 cgagtgcggt gagcgttccg atgcctggcg ccgggccttt tccgcggtcg aatccgaggc
     4321 gcaattccgc ccaatagaca agaccgagaa ccccgatgag tcccaacgtc aggaagggcc
     4381 agtccatctt gcgtgcggcg acagccggaa cggcgggacc cttgacgtcc gtcgcgggag
     4441 aaaccaccgg cttcggcgcc aatggaggcg cgaccgctac cggtttacgt tttccaaatg
     4501 cggtcgccgc ctgcatgcgt gcgctcccaa cgccgcaagg ggtttagcgc aaaggcgact
     4561 tcccaagcat gaacgtccgc accgaattat tcgcgtggta aacgaagaac taagacgaat
     4621 gtgggacggg ccgcgcctca gccttgtttc tcccattttc cggacgggct ttccttccaa
     4681 tatgtgaccg cgtgaccggc atccttcgca ccacgccagg cggcgcgcgc ggcagtcaga
     4741 gcctcgggat ctcgaccgtc gaacatgagc acaatgcgaa cgaactgagc gagcgacggc
     4801 gcgccccaat cgggaggcat cgcgcctccg accaggaaaa gcacctgcgc aaggttggga
     4861 ttttcctcct cggtcgtcag gtagatcggc tggcgcgatt tgtgtccttc ggccgccgta
     4921 ccatgggcga ggaacgtctg ttccgaatag gtccagagat gggcgtcgag cgcttccatg
     4981 cgttcggccg atcccacccg cacgagcgcg cgccaatcac gttcgcgcgt acgctccagg
     5041 aggccgggga gcacatcctc cagcgtacgg cgttcgagat gatagaacag ggtctcggac
     5101 attcacgaaa tcgccatgcc tcacgtctcg taattgtccg ccaccagccg gttcagcacg
     5161 cggacgcccc agcccgttcc ccaggagggt gcgagaggct tttgctctcc gtcctgccaa
     5221 gcgacgccgg cgatatcgag atgcgcccac ggaaccttgt tgacgaagcg ttgcagaatc
     5281 tgcgccgccg taatcgagcc cgcatgcggc ccgccgatat tcttcatgtc cgcgatcttc
     5341 gacttcacca gacggtcata gccatcgccg agcggcagcc gccacaccgg ctcgccttcc
     5401 gcggcccccg ctttgctgag gcgctccgaa agctcgtcat tgttgctgaa gagcccggca
     5461 tgttcggaac cgagtgccac caggatagcg cccgtcagcg tggcgagatt gaccatgaaa
     5521 cgcggcttaa agcgtgattg cgcgtaccat agcacatcgg cgaggacgag ccggccttcg
     5581 gcatcggtat tcaggatttc gatcgtctgg cccgacatcg tcttgacgat atcgttgggc
     5641 cgttgcgcct tgccgtcggg catgttctcc acgagaccga tgagtcccac cgcattcacg
     5701 tgcgctttgc gcttggcgag cgcatgcatg aggccgacga cagccgccgc accgcccatg
     5761 tcgcctttca tgcccatcat cccgccggcc gtcttgagcg acagtccgcc ggaatcgaag
     5821 cacacgccct tgccgatgaa ggcgagaggc gccttgtctt tgccggcacc tgatttgtgc
     5881 gcaccgttcc attgcatcac caccatgcgg gaatcatggg cgctgccctg gccgacgccg
     5941 agcagcgcgt tcatgccgag cttcttcatg gccgcaacgt caagcacctc gaccttcacg
     6001 ccgagcttct tcagcgccat ggtccgcgcc gcaaattcat ccgggtaaag aatgttggcc
     6061 ggctcgttca cgagatccct ggcgaggaaa acaccctccc ccacggcggc gagattgttc
     6121 cacgcctttc gcgaagctcc cggctcgggc gtcagcacaa ccaccttctt gagggtcggc
     6181 ttctcgtcgt cgcttacctt gcggtagtga tcgaagcgat agctgcgcag atgcgcgccg
     6241 aacgctagat gcgccgccag atcggcctct gcgattttgg cattcttggg acggtcgatg
     6301 gcgaaagtaa gtctctcttc gccgctggtc agaagccgcg ccaggattgc cgcggccaga
     6361 cgttcgacga cagcttcgtt gaagccatcc gctttgccga gcccggcaag cacgatgcgc
     6421 gaggacttca cgccggcagg cgccagcaat tcgagactat gccctgcctg acccttgaaa
     6481 cggctgactt gaagcgcgcg cttgacggca cccccggttg cccgatcggc gcgcatcgcc
     6541 gcatcgctca acaccccacc ttcaagaacg cctacgacga gggcgcccgg actcacggca
     6601 tcgggcgcgg cgaaagaaat ctccatgaac ggcaatgctc caaatcagat tcccggtatc
     6661 gggagatcag atatcagata ccacaggcgg gcgcgtaatt tttacgatac ctggtcccgg
     6721 ttttctgata cctgaatcgt atgcgaaagc tctttttcta tatcctgcgc cagatcgtcg
     6781 ggcctttcct gctgttcgca ctgatcctga cgctggtcgt gtggatgacc caatcgctcc
     6841 gcctcctcga cctggtcatc aatcgcggcc aatcggcggc catctttgcc tatctcacca
     6901 tgctgatgct gcccagcctg ctggtggtca tcgctcccat cgctttcttc ggggctgcgc
     6961 tctacgccct caacagactg aacaacgaca gcgaactggt ggtgatgtgg tcgtccggtg
     7021 tcagccggct ccagcttgcg ctgccggtgc tcgccgcagc catgctcgta atgacggtct
     7081 gctatgcctg cagcctctat ctgatgccgc tcggccagcg caccatgaag gacaaggtgt
     7141 tcgatatccg cgccgatatc ggggcggcca tcttacgcga aggcgcgttc acgacgccca
     7201 gcgatgggtt gacggttttc atccgcgaac tcgcgccgga cggtgaaatc cgcggcatcc
     7261 tcgtgcacga caatcgcgaa cccaagcgcc ccatcaccta tctcgccgaa tcgggcgtgc
     7321 tggcccaaac ccaggaaggc gcgcgcctga tcatggagaa cggaaatatc gagcagggcg
     7381 agggaggagg agcccggctc tcggtcctga agttcgaccg ctacgtgttc gatctcgatc
     7441 aatatgccgg cccgcagcgt gcctcggacc gcgacaccag cgagcgctac ctttccgagt
     7501 tgctcgatcc acctttcccg gccagtcaga cggttcggcg cggggtttat atcgccgaag
     7561 cgcacaatcg gctttcagga ccgctttatt gcatcgcctt cgcgttgatc gcactcgcgg
     7621 cgaccgcgaa aggacgcatg ggacgaacga gctacgcgct tcggctcgca ggcgcagcgc
     7681 tcataggcgc ggcgctgaga ctgatcggct atggggctca gggtatcgcg gcgagcaatc
     7741 cgcaattcac gttgatcctc tatctgctgc cgcttgccgc aagcgtggct gccgccggcg
     7801 tcctcacgga cataccgctg atacccgaaa gcatccgccg tctcatccgc gcacccgtgg
     7861 aggagcccgc atgacctggt cggtcacgct gatggtctac ttggccaaac ggtttctcgt
     7921 cgcggtgctg atcgtgttcg cggcattctc ggcactggcg ctgtcgatcg atctggccga
     7981 tcttttcagc cgcaccaccg agcgtggcat tccgcccgag atggtgatca gtatgagcct
     8041 gctcaagctt cccgacatcg cccagaagct gttgccgttc gcggtgttgc tcggcgcggt
     8101 gttcgtcttc tcgcggatgt cacgcagtca cgaactggtc gccacgcgcg cggcgggcat
     8161 atccgcctgg caattcctga cgccgccctt gctggtggcg gtggcgctcg gcgtggtgac
     8221 gatgacggcg ttcaatccga tggcatcggc attccttgcg caatatacgc ggcttgaggc
     8281 ccgctacatc cacggtcagg cctcgcagct tgcggtgtcc tcgacaggcc tgtggctgcg
     8341 ccagggcgac gccgcgcacc aatcggtggt tcatgcgctg cgggtcgccg atcagggagt
     8401 gcatctggaa gaggtcatcg tattcctcta tcaggggctc gatcattatg ccggtcgcat
     8461 cgatgccgca tccgccgagc ttcgcaatgg ggattggcat ttgaccaatg cttgggtcag
     8521 cggcgccgat ggggtacctc aacaccacga tgtctacgat cttcccaccg agttgacgcc
     8581 gtctcaaatt caggaaagct ttgcgtcgcc cgacacgatt tcgttctggg aactgccgcg
     8641 attcatcgcc aatgccgaag gcgcgggatt ttccgcgcta cgccaccggc tttattggta
     8701 ctcgttgctg gcgctgccgc tcctcttcag cgctatggtt ttcatggcgg cgagcttttc
     8761 gcttcgcctt gcacgactcg gcggaaccac acagctcgtg ctcgctgccg tgctctcggg
     8821 cttcggtgtc tatttcctcg gggacgtgac cggggccctc ggacaatcgg gcattctgcc
     8881 cacgccgctt gcggcgctgg cccctgcagc cgccgcgagc cttctcggca tgacattgct
     8941 ttttcacgag gaagacgggt gaaacgaaaa ggggggaaga aaccggggat tttccgcaag
     9001 gcccgtagcg tggtgacgct tgccgtcgcc ggagcgctgg gttctgccat gggaccgagt
     9061 gcggcagagg cgcagactag cgaatcgagc cgcgttctgc tgcagggcga caccgtcatc
     9121 tacgattcca acaccggaat cgtgacggcg gaaggtcacg tcgagatctc cgacgacgag
     9181 cgcacgctcc tcgcggacaa agtcacctat gacgaaaata gcaacaaggt caccgccagc
     9241 ggcaatgtca gcctgcagga ttcgaccggc aatgtcgcct atgcggattc cgtcgagttg
     9301 acgcaggatc tgcgcgaggg tgcgctgcag ggcttcgccg cgctgatcgg gaaaaccgga
     9361 cgcctcgccg catcctccgg cgaacggcgc gagggacgct tcaccatcgc ccatggcgcc
     9421 gtgttcaccc cctgctccat ctgccgtgaa gacggcgagc gcatgccgct atgggaaatt
     9481 cgggccgcgc ggatcgtcca cgaccagttg cagaaggaaa tctatttcga ggatgcaacc
     9541 ttcgaatttc tcggcctgcc agtcctgtac atgccatttt tctccgagcc cgatccgacg
     9601 atcaaataca aaagcggatt tctgcttccc gatatcggca gcatcggcgc catcggaagc
     9661 ttcatcaaga ttccatatta cgtctcgttg tcgccgtccc gcgatctgac aatcgatcct
     9721 ttcatcacca cccagggcgg aacggtgttg cagaccgaat atcgcgaacg ctggggcgcc
     9781 ggcggcctct ggcttcaggg aacgctgggg tatgattcgg aagcggacgg caaacctggc
     9841 gagagcatct ggatgggcag cctgttcggt tcgggccgcg tgccgctcaa cgacgatggc
     9901 tggcgcgccg gattcgatgt tcagctcgct tccaacaaga cctatctgaa gcgttacgag
     9961 ctctccaatc tcgatcgtct caccagcgat ctctttaccg acgatgtcga gggacgcagc
    10021 cgtgcggcga tcaccggtta tttcttccag agcatgcgag ccaccgacgt caccgcacaa
    10081 atgccgttgg cactgccgct gctcgaatac acctacatcc ccgaatacaa ggtctggggc
    10141 ggacggcttc aggccgacag cagcgcactc tatctcgacc ggaaccaggg taccgacatg
    10201 ctgcggggct cggccgatgt ggattggcgg cgtcccttca ccaccaatga cggggaactg
    10261 atcactttcg aaacattcct gcgcggcgat ctttattaca tcaacgacgc gaccttcgat
    10321 gtcccggggg cgccgaggaa cagcgaaacg atcggccgcg cgcttggcta tggcatgatc
    10381 gaatggcgtt ggcctttcgt cgccgatacc aacattccca acaccacgct ggtcgtggag
    10441 cctatcgctc aggtgatcgc cgcatcgggc ggcggaaatc ccccggggct gcccaatgag
    10501 gacagcaccg ctttcgaatt cgacagcagc aatctcttca gcgcgaatcc ggcgccaggg
    10561 ctcgatctct ggaccggggg gccgcgttcc aatgtggggt ttcgcgccag cgcgatcctg
    10621 ccgaatggct ccgttgaagc catgctgggc cagcagtttc gtttctcgac tgattcatcg
    10681 ctgccggtgg gcttgcgcct gaacgatgac aaatcggaca ttgtcggcca attcaagatt
    10741 cagtttccga acatatcgct gacccatcaa ttcaatatcg atcccgacga cggcacggta
    10801 cggcgcaatg aagtctatct gctggcgcat ttcggccgtt cgatgatcaa tctgagctat
    10861 ctgaagctgc cccagagtgc cgccgatccc tctctgggcg agcaggagca gatcaacctc
    10921 acaacgacgg tccaggttta cgggaattgg ggcattttcg gcgaagcgcg ccgcgacctc
    10981 gccaagaacc aggagcttga gagcagcatc gggattacct acgacgatga ttgctttgtg
    11041 gcgtcattgg ggttccatcg gcgcgacacc gcgacgttga acttgaagcc ctcctccgcg
    11101 gtgatttttc gccttggcct gaaaacaggc tatacgaaca gggcgccgtg aggcgacggc
    11161 ttgctcaatc gcttgaaccg cagggcgaaa tgggtagatt tccatttcgg gaggctgggc
    11221 gtggggtgcg cgagcgtcga agcgagagcg ggcgctgtta aacacagagt tggagaggat
    11281 gccgtgccgt atcgcctaga tgtgtttctg cgaactctgt gtttggctgg ggcagcattc
    11341 gcccccatgg ctgcggccgc gcaggatgcc gcgattccgc tgcagcctgg gttcctgtcg
    11401 caggttccgg gcgctccggt gaatcccacc ggacctgaag ccgaagcgcc cgtgattgaa
    11461 gtgccgagtg ccgaacccgc cccggccaca caacctcccg ccgcgaccac gccgcccagc
    11521 gatgctgcga cgccgccggc cgaagcgatg ccggcgattg cctcaccgcc ggccaccgca
    11581 tccgctgtgc ccgccaacgg tgtcgagccc aatgcaggca agcccggaga acgggtgaac
    11641 atcatcggcc cggcgaattc caccggggtc gcggccatcg tgaacgattt cgtgatttcg
    11701 aattacgatc tggatcagcg cgtcgcgctc ttcgtcacca cctcgggcgt gcggccgacc
    11761 agagacaatc tcccgcaaat ccgcgcccag gtcctgcgct cgctggaaga cgaagtgctg
    11821 gagcttcagg aggccaagaa gcataagctt accattgcca agaccgaggt cgacaaggcg
    11881 cttcagaata tcgcggagga caacaagctc accgtcgatc agatcctgaa aaccatcggt
    11941 caggcgggcg tcacccctac gacgttccgc caacaggtga ccgcgcaact gacgtggcag
    12001 aagcttgtgg ccgcccgtta cggtgcggat gtcaccgtct cggattctca ggtggatgaa
    12061 gccatgaccc ggctgaagca gggatcggac aagcctcagt tcctggtcgc tgaaatctac
    12121 gtcgccgtag accggccgga agatgagccg gcagttcgcg ccagcgtcga acagatcgca
    12181 cagcagatca agcaaggcgc tcctttccaa acggtcgcgg gtcaattcag ccagtcgccc
    12241 tcggctgccg atggcgggga tatcggctgg gtggtggagg gccaattggc tgaggaactc
    12301 gatcacgcgc ttgccgagct tcagccggga gagacgacag cgccgatccg cgcggaaggc
    12361 ggctattatg tgttgcaact gcgcgaccgg cgcgagccga tcggcaccgt cgtgaagccc
    12421 gcggcggcga cggcttccga tccggatgcg cctgtaccgc tggatcgtct gctgatccca
    12481 ttgccgcaaa atcccgagcc ccagctcaag gaacgcgcgt tgaccctggc ggccaatgtc
    12541 aaagctcagg tccggagctg cgcggagctt ccgaatgttg cgaatcagct ccagggctcg
    12601 gtttacaccc gactcggcaa catgaaccca aaggacctcg acccggcatt gcgcgacgcg
    12661 gtggccaaga ccagccccgg cgaaggcgtt gctcccttct tttcggccgc cggattggaa
    12721 ctcatcttcc gctgcgacgc cgcaccgccg aagctggtgg cgttcgaatt gccgacccgc
    12781 gaacagcttc agcagcagct tttcgtccag caaatgggaa tctacgcgaa aagctatttg
    12841 cgcgacctaa ggcgggatgc cgttgtcgaa acacgatgaa ggaagcggca agcttcccat
    12901 cgtcataagc cagggcgaac cggcaggcat tgggcccgag atcgccgtta aggcttggtt
    12961 catgcttggg gggcatgccg gcgaacacga attgcgcctg attggcagtg ccgatattct
    13021 ttctagagcg gcggtatcct gcggtatcga tccgcgtcct ctggcccacc acatcatcga
    13081 tacgggtcac ggcgtaaatg tccaatccgg acggccctcc gccggtaacg cgagggcggt
    13141 caccgccgcc atcgagcgtg gcgtcaacct atgccaatcg aacgaggcgg cagcactcgt
    13201 gacggcgccg atccacaagg ccgtactgac cgaagccgga tttggctttc ccggacacac
    13261 cgaattcctc gcggcgctga caggccgccc tcgggcgatc atgatgctgg catcgtcgga
    13321 gaccgatccg ccgcttcgcg tcgtgccctt caccatccat atcccgctga aggatatctt
    13381 cgccaagctc gacaccgatg ccatcgcaat gacggccgag atcgtgatcg acgcgctgtc
    13441 agccgatttc ggctgtccca acccgaggct ggcgattgcc ggtctgaacc cgcacgccgg
    13501 tgaggacggt acgatggggg ccgaagacag cgatattatt gcgccagcga tttcacgttt
    13561 gcgcgcgcgc ggtcatactg tgctggggcc gctgcctgcc gacaccatgt ttcatgagca
    13621 agcccgcagg tcctatgacg ccgcgctctg catgtaccac gaccaggcgc tgatcccaat
    13681 caagacgctc gctttttgga caggggtcaa tgtgacgctc ggtctgccga tcgtgcgcac
    13741 ctcgcccgat cacggcaccg cgctggacat cgccgggaag ggaatcgcca acccggctag
    13801 catggtcgcc gccatccgca tggctgcgga catcgccggc cggcggggtc tgtgaaccag
    13861 gaccgtatca gcgaggacgg cttgccgccc ctgcgcgagg tgatcgccgc gcatggactg
    13921 acacccaagc ggagcctcgg gcagaatttc attctcgatc tcaatctcac gcggcgtatc
    13981 gcgcgcgaag ctgagatcgc gggcgccgtt gtttatgaaa tcggccccgg tcctggggga
    14041 ttgacgcgcg cgcttctggc agaaggtgct gtgcgggtga ttgcggtgga gcgtgacggg
    14101 cgggctctgc cggcgctggc ggaaatcgca gcggccaagc cggggcgcct cgatgtcgtg
    14161 gaagccgacg caatggcaat agacgaacgc atgctgcttt ccggcattgg cgtggctctg
    14221 ccggtgccgg ccgtcgccaa tctgccgttc aatgtgggga cagcgctgct cgtcaaatgg
    14281 ctgagtgccg attggccgcc ctggtggcaa agtctgaccc tgatgttcca aaaggaggtg
    14341 gccca
//
LOCUS       DASYEG010000024         3065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4502527, whole genome shotgun sequence.
ACCESSION   DASYEG010000024 DASYEG010000000
VERSION     DASYEG010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4502527"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VGM72_01025"
     CDS             complement(<1..442)
                     /locus_tag="VGM72_01025"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEY4263873.1"
                     /translation="MSRTLNIAHRGGAKLWPENTLFAFVSAAQSGFDGAELDVQLTRD
                     GKLVVFHDFRLKPELCRSQDGRWLSGRRLALIRDLTLAELQDFDVGRPRPGSFYARRH
                     SKMTPRDGERMPSLSEVIEAVRAVKEDFRLFIEIKTSIGDRTLSQ"
     gene            553..1017
                     /locus_tag="VGM72_01030"
     CDS             553..1017
                     /locus_tag="VGM72_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263874.1"
                     /translation="MPMSKIALCAACLMVTGTAYAVNLPAENASGLVLHSTLGSLTGE
                     NSGIAIPTGEVQPGSTFVITGACVARVKSADHLRVVLTFADSSPDQPGYRSVVATDQE
                     IDAGGLAVRVPDFPEAANRVFSVKVFQLGESAPQMCDAGSIRIGGATPGKLG"
     gene            complement(1023..2588)
                     /gene="ubiB"
                     /locus_tag="VGM72_01035"
     CDS             complement(1023..2588)
                     /gene="ubiB"
                     /locus_tag="VGM72_01035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenylphenol 6-hydroxylase"
                     /protein_id="HEY4263875.1"
                     /translation="MFGSVRKTRRLLRMARILAAHDALLPREYAGRAPRSLRILARLL
                     GRPRMTGKTRPPGQRLSLALEQMGPSAIKLGQLLATRPDIMGAEIARGLETLQDRLPP
                     FADAQARAIVEAELERSLDTVFSSFGPPVAAASIAQVHEAETTDDPPRRVAVKILRPG
                     IEAQFVADFEGFRFAAKLGERFSAEARRLRLRTLVETLAASVALELDLRMEAAAASEL
                     AQNTRGDDQFRVPVLDWSRTSARVFTSEWVDGVVLRDPQTLTAAGHDPARIAVAVLRA
                     FLTQALRDGFFHADMHPGNLFVDRAGRICAVDFGIMGRLEIPMRRFMAETLAGFLARD
                     YMRVAQVHFDYGFVPKVHNVETFAQALRAVGEPIFGLTARDVSMARLLEQLFETTRRF
                     DMPLQPQLVMLQKTMVVVEGVARSLDPAFDIWEASRPVIENWMIDNIGPEARLRDAAD
                     GLTSLGRLAQNIPQLLRDTELIASQLAGGGLRLHPDSVRGIATGQLRRTRHVRVALWI
                     AAGALGIIALGLL"
     gene            complement(2591..>3065)
                     /locus_tag="VGM72_01040"
     CDS             complement(2591..>3065)
                     /locus_tag="VGM72_01040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HEY4263876.1"
                     /translation="VCDINLAMLEQGVKRPQALDETIVWVCGDAENLPIADSSQDAYT
                     IAFGIRNTTHLDRVLAEAYRVLKPGGRFLCLEFSRVAAPGLDSLYDLYSFAVLPRLGE
                     IVAGDGAAYRYLAESIRRFPAQTPFAKLIAKAGFTQVKFRNLSGGIAAMHSGWKV"
BASE COUNT          592 a         1007 c          953 g          513 t
ORIGIN      
        1 attgcgacag ggtccggtca ccgatgctgg tcttgatctc gatgaagagg cgaaaatcct
       61 cctttaccgc gcggaccgcc tctatcactt cggacaggga cggcatccgt tccccgtcgc
      121 gcggcgtcat tttcgaatgc cggcgcgcgt aaaaggagcc cggcctcggg cgcccgacat
      181 cgaagtcttg cagttcggca agcgtcaggt cgcggatgag cgccaaacgc ctgccgctca
      241 gccaacgccc gtcctggctt cggcagagtt ccggtttcaa gcggaaatca tgaaatacca
      301 ccaacttgcc atcgcgcgtg agttgcacat cgagttcggc gccgtcgaaa ccggattgcg
      361 ccgccgatac aaaggcaaaa agcgtgttct cgggccaaag cttggcgccg ccgcgatggg
      421 cgatattgag ggtcctgctc atctgctgca tgcggcaaaa ccggcgttga cgcgccttaa
      481 agaaaaatta ctatcggcaa gggcgcgctg ttgtgtaaac tgtgccgttg tcaaggcttg
      541 ggctggacaa aaatgcctat gtcgaaaatc gcgttgtgtg ccgcctgcct gatggtcacg
      601 ggcaccgcct atgccgtcaa tcttccggcg gaaaatgcaa gcgggcttgt gttgcacagc
      661 acgctcggtt cgctgaccgg cgagaattcc ggaatcgcga tccccacggg cgaggttcag
      721 cccggcagca cctttgtcat caccggggcc tgcgtggcac gggtgaaatc ggcggaccat
      781 ctgcgcgtcg tgctcacctt tgccgattcg agcccggacc aacccggcta tcgctcggtt
      841 gtggccaccg atcaggagat cgacgccggc ggtcttgccg tacgcgtgcc cgattttccc
      901 gaagcggcga atcgcgtctt ctcggtgaag gtgttccaac ttggggagag tgccccgcag
      961 atgtgcgatg cgggcagcat tcgcatcggc ggggcgacgc ccggcaagct gggctaatcc
     1021 cactaaagca accccagtgc gatgatcccc agagcgcccg ctgcgatcca taatgcgact
     1081 cggacatggc gcgtgcgccg caactgtccg gtggcgattc ccctgaccga atcgggatgc
     1141 agccgtaagc cgccgccggc aagctgagac gcgatcagct cggtatcgcg cagcaattgc
     1201 gggatgttct gtgcaagcct gccaaggctc gtgagcccat cggccgcatc gcgcagccgc
     1261 gcctcgggtc cgatattgtc gatcatccaa ttctcgatga cgggccgcga ggcctcccag
     1321 atgtcgaagg cgggatcgag gctgcgtgcg acaccttcca ccacgaccat ggtcttctgg
     1381 agcatcacga gctgcggctg aagcggcatg tcgaagcggc gcgtggtctc gaagagctgc
     1441 tccagcagcc gcgccatgga gacatcgcgc gccgtcaggc cgaagatcgg ctcgccgacc
     1501 gcgcgcaagg cttgcgcgaa cgtctccaca ttatggacct tcggcacgaa gccataatcg
     1561 aaatgcacct gcgccacgcg catataatcg cgcgccagga agcccgcgag cgtttcggcc
     1621 atgaagcggc gcatcgggat ttcgagccgg cccatgatcc cgaaatcgac cgcgcagatg
     1681 cggcccgcgc gatcgacgaa cagattcccg ggatgcatgt cggcatggaa gaagccgtcg
     1741 cgaagcgcct gcgtgagaaa cgcgcgcagc accgccaccg cgatgcgcgc cggatcgtga
     1801 cccgccgcgg tgagggtttg cggatcgcgc agcaccacgc cgtccaccca ttccgacgtg
     1861 aagacccgcg cgctcgttcg cgaccaatcg agcaccggca cgcggaactg atcgtcgccg
     1921 cgcgtatttt gggcaagctc cgaggccgcc gccgcctcca tgcgcaaatc cagttcgagc
     1981 gcgacggacg ccgccagcgt ctcgaccagc gtgcgcaagc gcagacggcg ggcctcggcc
     2041 gaaaagcgtt cgcccaattt cgccgcgaaa cgaaagcctt cgaaatcggc cacgaattgc
     2101 gcctcgatgc cgggacgcag gatcttcacc gccacgcggc gcggcggatc gtccgtggtc
     2161 tcggcttcat gcacctgcgc gatggaagcc gcggccacgg gcgggccgaa tgacgagaac
     2221 accgtatcca atgaccgctc gagttcggct tcaacgatgg cacgcgcctg tgcatcggca
     2281 aaaggcggca ggcggtcctg aagcgtctcc agcccgcgcg cgatctctgc gcccatgata
     2341 tcgggccgcg tcgcgagaag ctggccgagc ttgatcgccg acggccccat ctgttcgagc
     2401 gcaagggaaa gccgctgacc cggcggacgc gttttcccgg tcatcctcgg acggccgagc
     2461 agacgggcca aaatgcgcaa cgagcggggc gcgcggccgg cgtattcgcg cggcagcagc
     2521 gcatcatgcg cggccaatat gcgcgccatg cgcaacagcc gcctcgtttt gcgcacactg
     2581 ccgaacatgc ctacaccttc cagcccgagt gcatggccgc aattcctccg gacagattgc
     2641 ggaacttcac ctgcgtgaag cccgccttcg cgatcagctt cgcaaatggc gtctgtgccg
     2701 gaaaacgccg gatgctctcg gccagatatc tgtacgccgc gccgtcgccg gccacgatct
     2761 cgcccaatcg cggcagcacc gcgaaagaat aaagatcgta aagactgtcg aggcccggcg
     2821 cggcgacgcg cgaaaactcc aggcacagaa agcgtccgcc gggtttcaac acgcgatagg
     2881 cctcggcgag cacgcgatcc agatgcgtgg tgttgcggat gccaaacgcg atcgtatagg
     2941 cgtcctggct cgaatccgcg atgggcaggt tttccgcatc gccgcagacc caaacgatgg
     3001 tttcatccag cgcttggggc cgctttaccc cctgctccag catcgcaaga ttgatgtcgc
     3061 agacc
//
LOCUS       DASYEG010000025         8014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_879687, whole genome shotgun sequence.
ACCESSION   DASYEG010000025 DASYEG010000000
VERSION     DASYEG010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 8014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8014
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879687"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..749)
                     /locus_tag="VGM72_01045"
     CDS             complement(219..749)
                     /locus_tag="VGM72_01045"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY4263877.1"
                     /translation="MSEARLLALVLVFAPLSLLSFGGGQAIVADMQHQVVDVQHWISG
                     PEFADLFALSRAAPGPSTLIAALIGWQVAGFLGAIVATLAIYIPSSFVVYGAVRWWHA
                     SKQSPWRGALERGLMPVAVGLVFAGALAVLQAAHVNGLQIFTTVVVTGLLVSTGVSIY
                     LIMAVTALAFGLLQWL"
     gene            complement(746..1312)
                     /locus_tag="VGM72_01050"
     CDS             complement(746..1312)
                     /locus_tag="VGM72_01050"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY4263878.1"
                     /translation="MTSAPLAQKPLVPLLDLMAVFFRVGVTSFGGSTAAWLYRELVER
                     RGWLDEDHFLTALTLAQVLPGANPVNLSIYVGSQLRGGIGGAVAALGMIGPPFCIILT
                     LGFLYREFGSSPIAHAVLGGLAAVGVGMTLSIGVKLAQKVRKPVPVLIAAALFVTVGI
                     LHWPMIPVVIVLTPLSIGLERFAGRNSS"
     gene            1557..2750
                     /locus_tag="VGM72_01055"
     CDS             1557..2750
                     /locus_tag="VGM72_01055"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263879.1"
                     /translation="MKKQVFTGARLAALAFAICGSNDVVAADAQKPAVPIFEVDSHFP
                     TMPDNMLLGGVGGATADSHGNVWVIHRPHTLEEGNATENGYLPAPPVVEFSAAGKYLQ
                     GWGGPSKDGKYQWFNRGGLHSAFAECPSCTAEKRLNGDGRPGSGEHGIAVDGHDNVWL
                     TGNGDGDGQVLKFTSDGKFLLQIGKEGVGVDSNDTTHLSRPAGITVYDKTNEVFVADG
                     YGNRRVIVFDANTGAYKRYWGAYGKKPDDKASKARQTTGPGPLQFNTPHGIEVSNDGI
                     VYVTDRANNRVQSFKLDGTFLKEGFIRRESQGTGTAFGVGLSADPAQRFLYIADGSNE
                     RIAILDRASLVEIGHIGRPGRKAGEFFHLHSLDVDPQGNIVTGESQGYRVQKFLFKGL
                     STDRR"
     gene            3010..3750
                     /locus_tag="VGM72_01060"
     CDS             3010..3750
                     /locus_tag="VGM72_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4263880.1"
                     /translation="MPRRSSESEYAISQPRIGGAIAGESAARPKLFIVSDIRLLCDGL
                     TLALSQSSTVNVLGFGDLSISPGYLAGLDPDVLLLDIGSSSALATCRGFRQALPRVRV
                     VAIAVGEIEQNVIACAEAGISGFVPRQGTAQDVVVAVQSALRDELVCSPRMTALLLNS
                     IAVMSGRKSSPTDVAALTEREQEIASLVGEGLSNKEIARALRIQNATVKNHVHSILGK
                     LRVRRRGEAAAQLHKLHPHHSPSNAPPR"
     gene            4037..4447
                     /locus_tag="VGM72_01065"
     CDS             4037..4447
                     /locus_tag="VGM72_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263881.1"
                     /translation="MKRIRVLLARMPALLLDILHHIVESAPDMMIVGESADNDLAGAA
                     KRLRADAILVGEDANHDSGEYTQLLLSRPSLKVLAIANEGRNGTLHEMRPQHIPLGEI
                     SAETLRDAIRGRHGRDAQESGAHAVRSSSSRKER"
     gene            4456..5940
                     /locus_tag="VGM72_01070"
     CDS             4456..5940
                     /locus_tag="VGM72_01070"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEY4263882.1"
                     /translation="MPQPLLQPGVYIEEISSGVRTITGVATSVALFLGWAARGPADRA
                     VRIASFSDYQRTYGGLDLHTPLGYSVKQFFDNGGSDAYVLRLVGAGAAPATVTIGTLA
                     ISASSPGLWGTGYAVRTTQRPSPDNARFKVEVLDKNNGNAVVESFENVSMDSSDLRFV
                     ESVINNRSAFITVSAGSTAAPANTVSDLAGAAADGTILAPSTPDFHTALLANFGIGSI
                     TDRIDIFNLVCVPGETDAATLATLQGHCHDRRAFLIVDADPLATVTSLNSGAPAALTG
                     SNAVNSAIFFPWVRAPDPLAQGALVDFPPCGFVAGVFARTDASRGVWKAPAGSEASLN
                     GAVGLSITMSDAENGQLNPLGINCLRTLPVYGNVVWGSRTLNGENDRGSEWKYVPVRR
                     MALFLEESLYRGTQWVVFEPNDEPLWAQIRLNIGAFMQGLFRQGAFQGQSAKDAYFVK
                     CDRETTTQADINLGIVNIFVGFAPLKPAEFVVIKIQQIAGDIPT"
     gene            5962..6486
                     /locus_tag="VGM72_01075"
     CDS             5962..6486
                     /locus_tag="VGM72_01075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEY4263883.1"
                     /translation="MAQFSVNPQRFDPYKNFKFRVKWDGRYVAGISKVSSLKRTTEVV
                     EHREGGDPSTGRKSPGRTKYEAITLERGVTHDTEFEKWANKVWNFGSGLGAEVSLQDF
                     RKDLIIEFYNEAGQLAIAYRVFRCWVSEFQALPDLDANANAVAIQHIKLENEGWERDL
                     DVTEPSEPSFTEPA"
     gene            6499..7212
                     /locus_tag="VGM72_01080"
     CDS             6499..7212
                     /locus_tag="VGM72_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263884.1"
                     /translation="MHSPAREAREHRILMLWECAVRLKRWARDDALLEAAGNRSEGLG
                     ARNAALLSLRSALFDSAWPLLSRCPECSTICEFEADSTDLASALSGLSVGEESVIDAG
                     GEQIRLRAPTAADLRAISTLADQGAAARSLLALCVSPVRDGESFDAAQLVELGLELER
                     LDPGALVSFALSCPACRHDWSAAVDVGEALWAELQRAAEKIFVDIDVLARAYGWTEAD
                     VLGLSAVRRAAYLQLAGTP"
     gene            7209..7898
                     /locus_tag="VGM72_01085"
     CDS             7209..7898
                     /locus_tag="VGM72_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263885.1"
                     /translation="MSGFLDRLAGLATGRPVEGGARVALPSRFAPVSSAPLPNVEFAT
                     TDDVSAAPKSPVHVAENAATVRPLEESREASFQAAFVTARIDASPVRLENRPAPAEPP
                     RAVVTPKMGVTIPQQPPRSRAVQESSASMLGPRPPEDPSRIHPPLRASAIAARGAEIS
                     NRAPLNADILTTRPQAAHNVPPPIHVTIDRIDVRVAAPAKTAPAQTRVRRGPSQSLAE
                     YLRNSQRGGNS"
BASE COUNT         1568 a         2493 c         2487 g         1466 t
ORIGIN      
        1 cactgtaaga gccacgcatc aattcgaaat cccaccggta accctgcaga gacttacatc
       61 caatgatcct gcaaccgatt tggctctgag tttcgacctg accgaacagc acaatgacgc
      121 cacatagcgc caccgcaaca gacttaaaac cgagcacttc ggcgcaaacc acctagaaaa
      181 acgcttaggt gtgtttgcta cctaatgccg gaacgatctc agagccattg cagcagccca
      241 aaggcgagcg cggtcacggc catgatcaga taaatgctga cgccagtgga cacgagcagc
      301 ccggtcacga cgacggtggt gaatatctgc aggccgttca catgggccgc ctggagcact
      361 gcgagagctc cggcgaacac cagtcccacg gcaaccggca tgagcccgcg ttccagcgcg
      421 ccgcgccagg gcgattgctt gctcgcatgc caccagcgca ccgcgccata gaccacgaac
      481 gaggacggaa tatagatcgc aagcgttgcc acgatggcgc ccagaaaacc cgccacctgc
      541 cagccgatca atgctgcgat cagcgtgctg gggccgggag cggcgcgcga cagcgcgaag
      601 agatcggcga attcgggacc gctgatccaa tgctggacgt cgacgacctg gtgctgcatg
      661 tccgcgacaa tcgcctggcc gccgccaaat gaaagcagcg acagcggcgc gaacaccaga
      721 acaagcgcca gtaaccgggc ttcgctcatg aggaattcct accggcaaag cgttcgagcc
      781 cgatggaaag cggggtcagc acgatcacca ccggaatcat cggccaatgc agaatgccga
      841 cggtcacgaa aagcgctgcc gcaatcagca cgggaaccgg tttgcgcacc ttttgcgcca
      901 gtttgacacc aatggagagc gtcatgccca cgccgacggc cgcaagtccg cccaggaccg
      961 cgtgcgcgat gggggacgat ccgaattccc ggtagaggaa cccaagcgtc aggatgatgc
     1021 aaaagggggg gccgatcatg ccgagcgccg ccacggctcc accaatccct ccgcgaagct
     1081 gcgagccgac atagatcgaa agattgacgg gattggcgcc cggaaggacc tgtgcgagcg
     1141 tgagcgccgt cagaaaatgg tcctcgtcga gccagccccg gcgctccacc aactcgcgat
     1201 agagccaggc ggccgtgctg ccgccgaaac tggtgacgcc gacgcgaaaa aaaacagcca
     1261 tcaagtccag gagcggaacg agcggttttt gcgccagggg agcggaggtc atggaaagct
     1321 cgaggacgat acccgtccga gatttagcat cctgatacgg agtgtccatc gctgcggtga
     1381 cgcgggcctt cgccgtcacc cctccagcta agcgaatggc attatcgcaa agccgggata
     1441 atctgttgcg atcgcgggag gtcgccatgg ccgcacgtca gccgattgct gacgggtttt
     1501 ctcaaggatc aggcggtgcc gcaagaacgg ctgaagatta cgcgggagga aagaacatga
     1561 aaaaacaggt cttcacgggc gcgcgtttgg cggcattggc atttgcgatc tgcggctcca
     1621 atgacgtggt tgcggccgat gcccaaaaac cggccgtgcc gattttcgaa gtggattctc
     1681 atttccctac aatgccggac aacatgctgc tgggcggcgt gggcggtgcg accgcggatt
     1741 ctcatggtaa tgtctgggtg atccaccgcc cccacacgct ggaggagggg aacgcgacgg
     1801 agaacggata tcttccagcg cctcccgtcg ttgaattcag cgccgccggc aaatatctcc
     1861 agggctgggg cggcccgtcc aaagacggca aatatcaatg gtttaatcgt ggagggctgc
     1921 attcggcgtt cgccgagtgc ccgtcctgca ccgccgagaa acgcctgaat ggcgatggac
     1981 gtccgggcag cggcgaacac ggcatcgcgg tcgatggcca cgacaatgtc tggctgacgg
     2041 gcaatggcga cggcgacggg caagtgctca aattcaccag tgatggaaag ttcctcctgc
     2101 agatcggcaa agagggcgtg ggcgtcgaca gcaacgacac cacccatctc agccggccgg
     2161 cgggcatcac cgtctatgac aagaccaatg aggtgtttgt cgccgatggc tacggcaacc
     2221 gccgtgtgat cgtgttcgac gccaataccg gagcctacaa gcggtattgg ggcgcctatg
     2281 gcaagaaacc ggacgacaaa gcttcgaagg cgcgccagac caccggtcca gggccgcttc
     2341 agttcaacac gccccacggc atcgaggtct ctaatgacgg catcgtctat gtgacggatc
     2401 gcgccaataa ccgcgtccaa tcgttcaaat tggacggcac cttcctgaag gaaggcttca
     2461 tcaggcgtga atcgcaaggg acaggaacag cctttggcgt cgggctttcc gccgatccgg
     2521 cgcagcggtt cctttacatc gcagatggct ccaacgaacg gatcgcgatc ctggatcgcg
     2581 cctcgctggt ggagatcggt cacatcggca ggcccggccg gaaagccggc gagttcttcc
     2641 atctgcacag cctcgacgtc gatccacagg gcaatattgt gaccggcgag tcgcagggct
     2701 accgcgtgca gaaattcctc ttcaagggat tgtcgacgga caggcgataa ttaccggtca
     2761 agacgcttct ggtatcgtca agacaaacga gaggcggacc ttattttgtc acaggcgaag
     2821 cgttgtttct gcgcaaggtg actttgcatt tcaggtcatg gccggcttct tggcttctca
     2881 tgaaatcttc acacaaattc gactccacag ttttgtgttt cacgaatagt atcgaagggc
     2941 tccgctgtcc tttcgagggg aaaaagcgac cgtgggtttc tggttgtgtc cagcgagcgg
     3001 gggcttctga tgccgaggcg aagcagcgaa tcggaatatg cgatttcgca accgcgtatc
     3061 ggtggcgcga tcgccggcga gtcggcggct cgcccaaagc ttttcatcgt gagcgacatc
     3121 aggctgttgt gcgatgggct cacgcttgca ttgtcccaat cgagcacggt gaatgttttg
     3181 ggtttcggcg atctttcgat ttcgcccggc tatcttgccg gattggatcc cgacgtgctg
     3241 ctgctcgaca tcgggtcttc gagcgcgctt gccacctgcc gcggcttccg ccaggcgcta
     3301 ccgagggtga gggtggtcgc catcgcagtc ggcgagatcg aacagaacgt catcgcctgt
     3361 gcggaagcgg ggatttcggg tttcgtcccg cgtcagggca ctgctcagga tgtcgtcgtg
     3421 gctgtgcaat cggctctgcg cgacgagctt gtctgttcgc cgcggatgac ggcgctgctc
     3481 ctgaacagca tcgcggtcat gtcgggacgg aaaagttcgc ccaccgatgt ggcggcgctc
     3541 acggagcgcg aacaggagat cgcgtctctc gtcggtgaag ggctctcgaa caaggaaatc
     3601 gcgcgcgccc tgcgaatcca gaatgcgacc gtgaaaaatc acgtccacag cattctcggc
     3661 aaactgcgtg tgcgccgccg cggcgaggcc gcggcacagc tccataaact ccatccgcat
     3721 cattccccga gcaacgcgcc gccgcgttag agcgcaatcg ggcgaagtgg ctgcttcgtg
     3781 cattcgccaa aacgcagtgc aggaacggga ccgggccaaa cggtctatga cgcgtaggcc
     3841 ttttgtaaaa ccgcattgat ggaattctta ccgcttttcc acgttttgcg cgcggcccgc
     3901 gacgcctgac ggtctatctg cccgcgcggc gcattccgtg cggtccggat ctttgccgga
     3961 tccttgtctg agccgaaggg ttcattcgcg ttcaccgggg aactgccatg ctccgcggca
     4021 cggaaaggga atcctcgtga agcggattcg tgtgctgctt gctcgaatgc ccgctctgct
     4081 gctcgacatc cttcaccata ttgtggagtc ggcaccggat atgatgatcg tcggcgagag
     4141 tgcggacaat gaccttgccg gagcggccaa gcggctgcgc gccgacgcga ttctggtcgg
     4201 cgaggacgcc aaccacgatt ccggagaata cacgcagctt ctgctgagcc ggcccagtct
     4261 caaggtgctc gccatcgcca atgagggcag gaacggcacg ctccacgaaa tgcggccgca
     4321 gcacattccg ctcggggaaa tctccgcaga aacgcttcgt gacgccattc gcggccgcca
     4381 tggccgcgat gcgcaggaaa gcggcgccca tgccgtgcgc agttcatctt cgaggaaaga
     4441 acgataggag ccgatatgcc ccaacctctt ctccagcccg gtgtctatat tgaagaaatc
     4501 tccagcggcg tgcgcaccat caccggtgtt gcgacctccg tcgcgctgtt cctgggctgg
     4561 gccgcgcgcg gcccagcgga ccgcgccgtg cgcatcgcga gcttctccga ttatcagcga
     4621 acctacggcg gattggacct gcacaccccg cttggctatt ccgtcaagca gttcttcgac
     4681 aatggcggca gcgatgccta tgtgctgcgc ctcgtgggcg ccggcgcggc gccggccacg
     4741 gtcactatcg gcacgcttgc gatcagcgcg tcgtctccgg gtctctgggg aacgggctat
     4801 gcggtccgca ccacgcaacg gccctcgccc gacaatgcgc ggttcaaggt cgaggtgctc
     4861 gacaagaaca acggcaatgc cgtcgtcgag agcttcgaaa atgtttcgat ggacagcagc
     4921 gacctgcgct tcgtcgagag cgtgatcaac aaccgttccg cgttcatcac agtcagcgcc
     4981 ggcagcacgg ccgcgcccgc caatacggtc agcgaccttg ccggtgccgc ggcggacggc
     5041 accattctgg cgccctccac gccggacttc cacacggcac tgctggcgaa tttcggcatc
     5101 ggcagcatca ccgaccggat cgacattttc aatctggtct gtgtgcccgg cgaaaccgat
     5161 gccgcgacct tggcgacgtt gcagggtcat tgccatgacc ggcgcgcctt cctcatcgtc
     5221 gatgcggatc cgctggcgac ggtcacctcg ctcaattccg gagcacccgc ggcgctcacc
     5281 ggctcgaatg cggtgaactc ggcgatattc tttccctggg tccgcgctcc cgatccgctg
     5341 gcgcaaggcg cgctcgtcga tttcccgccc tgcggtttcg tcgccggggt gttcgcgcgc
     5401 accgatgcct cgcgcggtgt gtggaaggcg cctgccggca gcgaagcctc gctcaacggg
     5461 gcggtcggtt tgtcgatcac catgagcgat gcggaaaacg gccagctcaa tccgctcggc
     5521 atcaattgcc tgcgcacgct ccccgtttac ggcaacgtcg tgtggggttc gcgcacgctg
     5581 aacggcgaga acgaccgcgg ctcggaatgg aaatacgttc cggtgcggcg catggcgctg
     5641 ttcctggagg agagcctcta tcgcggcacg caatgggtcg tgttcgagcc caatgacgag
     5701 cctctatggg cgcaaatccg gctgaacatc ggtgcgttca tgcagggtct ttttcggcaa
     5761 ggcgcattcc agggccaatc ggctaaggac gcctatttcg tgaagtgcga ccgcgagacc
     5821 acgacgcagg ccgacatcaa tcttggcatc gtcaatatct ttgtcggttt cgcgccgctc
     5881 aaacccgccg agttcgtcgt catcaagatc cagcagatcg ccggggacat tccgacgtaa
     5941 agcgctgaga ggagaaattt tatggcccag ttttctgtca atccgcagcg cttcgacccc
     6001 tacaagaact tcaagttccg ggtgaagtgg gatgggcgct atgtggcggg aatcagcaaa
     6061 gtctcgtcct tgaagcgcac caccgaagtg gtcgaacacc gcgagggcgg cgatccatcc
     6121 accgggcgca aatcgcccgg acgcaccaaa tatgaggcga tcaccctcga acgtggcgtc
     6181 acgcatgaca ccgaatttga gaaatgggcg aacaaggtgt ggaatttcgg ttccggattg
     6241 ggcgccgaag tctcgcttca ggatttccgc aaagacctta tcatcgagtt ctacaacgaa
     6301 gccggacagc tcgcgattgc ctaccgcgta ttccgctgct gggtgtccga attccaggcg
     6361 cttcccgatc tcgatgccaa cgccaatgcg gtggccatcc agcacatcaa gctcgagaac
     6421 gagggctggg agcgcgatct ggacgtgacc gagccgagcg aaccgagctt caccgagccg
     6481 gcgtgagggg ggtagcgcat gcattcgccg gcgcgagagg cgcgcgagca ccggatcctc
     6541 atgctgtggg agtgcgccgt ccggctgaag cgctgggcgc gagacgatgc gttgcttgaa
     6601 gcggccggca accggtccga gggtctcggc gcacgcaatg ccgcgcttct gtcgttgcgc
     6661 agcgcgttgt tcgattccgc ctggccgctt ttgagccgct gtccggaatg cagcacgatt
     6721 tgcgaattcg aagccgacag cacggacctt gctagtgcgc tctcgggtct ttccgtcggt
     6781 gaggaatccg tgatcgatgc cggtggcgaa caaatccggt tgcgggcacc gaccgcggcc
     6841 gatctgcgtg cgatttcgac gcttgcggat caaggcgcgg cggcgcgttc tctgcttgcg
     6901 ctttgtgtgt cgcccgtgcg cgatggtgaa tcgttcgacg cggcccaatt ggtggaactc
     6961 gggctcgagc tcgaacgcct cgatccgggc gcgctggtga gttttgcgct ttcctgtccg
     7021 gcctgccgcc atgattggtc ggcggcggtc gatgtcggcg aagcgctatg ggccgagttg
     7081 cagcgcgcgg ccgaaaaaat cttcgtcgat atcgacgtct tggcgcgcgc ctatggctgg
     7141 accgaagcgg acgtgctcgg cctgtctgcc gtccggcgcg ccgcttattt gcaactggcg
     7201 ggaacaccat gagcgggttc ctcgatcgac tcgccggcct cgcgacaggc cgaccggtgg
     7261 aaggcggcgc gcgtgttgcc ttgccgtcgc gcttcgcgcc ggtttcatcc gcgcccttgc
     7321 ccaatgtgga attcgccacg acggacgatg ttagcgcggc gccgaaatcg ccggttcacg
     7381 ttgccgagaa tgccgcgacg gttcgtccgc ttgaagaatc gcgtgaagcc tcatttcaag
     7441 ccgcgttcgt gaccgcgcgg atcgacgcgt ccccggtgcg tctggaaaac cgccccgcgc
     7501 ctgccgagcc gccgcgcgcc gttgtgacgc cgaaaatggg cgttacgatt ccgcagcagc
     7561 ctccgcgctc ccgcgccgtg caagaatcgt cggcgtctat gttgggcccc cgcccgcctg
     7621 aagacccatc gcgaatacac ccaccgcttc gcgcgtcggc gatcgcagcg cgcggcgccg
     7681 aaatttccaa cagagcgccg ctgaatgccg atatcctgac cacacgtccc caggcggcac
     7741 ataatgtccc gccgccgatc catgtcacca tcgaccgcat cgatgtgcgt gtggcagccc
     7801 ctgcaaaaac agcaccggcg cagacccgcg tgcggcgcgg cccaagccag tcgctcgcgg
     7861 aatatctgcg taactcccag cgcggaggca actcatgagt tcgccgctcg cgatcggagc
     7921 tgtcagcgcc gtattgcgca acctcctcga taacggtctg atcgaggcgg gtgccgcgat
     7981 gggtacgacg gtcaacatca gcgcggtcgc gccc
//
LOCUS       DASYEG010000026        14989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4648476, whole genome shotgun sequence.
ACCESSION   DASYEG010000026 DASYEG010000000
VERSION     DASYEG010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 14989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14989
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4648476"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..2391)
                     /locus_tag="VGM72_01090"
     CDS             complement(211..2391)
                     /locus_tag="VGM72_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY4263886.1"
                     /translation="MRTKRAACLGMMSASFVALLCAASATTAVAQPAEQVKIDADDIG
                     GVVTGAKGPEAGVWVIAETRDLPTKYVKIVATDDQGRYVIPDLPKGTYDIWVRGYGLV
                     DSPKVKATPGKLVDLKSVAAPNRAAAAAYYPGIYWYAMMKIPPANLFPGTGPQGNGMS
                     VTLKDQGQWLRFTKTDGCGHCHQLGDLATRTLPADLGHFNTSAEAWERRLESGQAGRN
                     MMEELAKLDVQRQLNFLGDWTDRVAKGELPKNDPPRPVGMERNVVLTEWDWETPTMYV
                     HDTISTDRRNPTVNGHGLIYGAPEVSSDNVPWLDPVDNKSGFIKSEFRDPKTPTSKTD
                     LILAPSPYWGNEPIWDSHTIIHNPMFDENGRVWLTARIRPQANPAFCKEGSDQPSAKI
                     YPKNTSNRQVEMYDPKTRKISMIDLCFNTHHLQFDKNDILWFSAGGNYDVIGWLDVKK
                     WEATHDDKMSQGWSPFVIDTNGNGKRDVSWTEPNQPADPAKDKRMFLGFYGVAPNPAD
                     GTIWGGVTGFPGGIARFDPKTQLTEYYEFPYKNAKNPQSGFSPRGSDIDSNGVFWAVL
                     SSGHLASFDRHLCKGKLNGPAANDPQNLCPEGFKLYQMPGPEFESLPKGTPGASAEAP
                     YYVWVDQHNTFGLGENVPIVTGNNSDSLNALINGKWMVMRIPYPLSFYAKNLDGRIDD
                     MATGWKGRGLWTQYSGRSQPHLEGGKGQQSKVVHVQYRPDPLAK"
     gene            complement(2549..4720)
                     /locus_tag="VGM72_01095"
     CDS             complement(2549..4720)
                     /locus_tag="VGM72_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY4263887.1"
                     /translation="MRTRTANIGMLSTSVAALLFAVSATSATAQQVRVDASDIGGVVT
                     GAKGPEAGVWVIAETKDLPTKYVKIVVTDDSGRYMLPDLPKGNYDIWVRGYGLVDSPK
                     VKSAPGKVLDLKAVAAPSRAAAAEYYPAQYWYSMVKPPASNLFPGTGPAGNNMSQQMS
                     DQGVWLRNMKTDGCVACHQLGDKATRTIPAALGHFDSGAAAWERRLQSGQASGPMMGA
                     IGRFDTQRALSMFADWTDRVSKGELPKSDPPRPAGLERNIVVTEWDWNTPKAYLHDEI
                     STDRNNPGVNAHGIIYGSTEASSDYIPWLDPVDNKAGLLKTEYRDPKTPTTKNDAIYG
                     ESPYWGNEPIWDSHTNVHNPMYDSQGRLWLTARIRPQNNPAFCKEGSQQASAKIFPQG
                     RSGRQVEMFDPKTRKITMVDLCFSTHHLQFDKTGMLWFSSGGGNNEVIGWLDVKKFDA
                     THDEQASQGWSPFVIDTNGNGKRDAGWVDPKGKLDPTKDMRIVTGLYSVSPNPADGSV
                     WGTVLGFPGGVARFDPKTQLTEYFEVPWKNAKAETMGFSPRGADITSDGVVWTVLASG
                     HFASFDRRLCKGKLSGPALADPQNLCPEGWKLYQMPGPEFEGLPKGTPGAGTEAPYYN
                     WVDQYDTLGLGKNTPIATGNESDSLAAIVNGKWVVMRVPYPIGYFAKGMDGRIDDAAT
                     GWKGKGLWSTFSDRAATHIEGGKGQMSKVVHFQLRPDPLAK"
     gene            4891..5145
                     /locus_tag="VGM72_01100"
     CDS             4891..5145
                     /locus_tag="VGM72_01100"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEY4263888.1"
                     /translation="MDDYPRKGAEEARNQLPDLLDAAENGKTTIITRHGRPVAALTPV
                     DVLSAAREQQPLTPLKGSGTGLWGKNSTHTLRALRDEWHN"
     gene            5133..5558
                     /locus_tag="VGM72_01105"
     CDS             5133..5558
                     /locus_tag="VGM72_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY4263889.1"
                     /translation="MAQLDLADLPAGALLLIDSAPVIYFMEAHPKWASRFRPVFEAHA
                     AGRFRFAVTTITIAEVLIGPFKAGDEVLGRRYRSVLESWQRIDLDLDIADSAARLRAS
                     LGFKLPDAIQVASALKINAAALVTHDRDFSRVRSLRVIS"
     gene            complement(5641..7821)
                     /locus_tag="VGM72_01110"
     CDS             complement(5641..7821)
                     /locus_tag="VGM72_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263890.1"
                     /translation="MSTAGRSCLSMLAASLMGTALFIAAPLPASAQPAGGAVQVKADE
                     IGGVVTGAKGPEAGVWVIAETKDLPTKYVKIVVTDDSGRYLIPSLPRANYDVFVRGYG
                     LVDSARTKTALGKTVNLKSTAAPNETAAAEYYPAIYWYSMVKVPEPKLFPGTGPSGNG
                     MPVSLTDQGMWLRYMKTDSCNSCHQIGDKATRELEPQLGHFATSAAAWERRLQSGQAS
                     ALMMNGIGNFDTARALANLGDWTDRVKKGELPFAKPTRPTGIERNVVLTEWDWATPQS
                     YLHDEISTDRRNPTLNPHGLIYGSPEESSDFIPWLDPVDNKSGLIKSEYRDPKTPTTK
                     TAPQYGESPYWGTDAIWDSHTVIHNPWMDEKSRLWVTARIRPANAEPAFCKAGSNHPS
                     AKAFPLSRSGRQLEVYDPQTKKATPIDLCFSTHHLQLDKNNVMWFSSGNTNDEVVGWF
                     DVKKWDATHDEQASQGWAPFVVDTNGNGKLDEYVGPKDAVDPKKDKHVQVGFYGASPN
                     PADGTVYASYLGFPGGVARFDPKTKLTEFYEVPYKDPKNPRSGFSPRGMDIDSNGVAW
                     LALASGHMASFDRKLCKGPLNGPIAATGKACPEGWKFYTIPGPQFKGITVTDGSAEAP
                     YYDWVDQFDTLGLGKNVPIATGNLSDALFALVNEKWVTMRVPYPMGFFAKNMDGRIDD
                     SSTGWKGKGIFSTYSGRSAPHIEGGKGQTSKVVHFQVRPNPLAD"
     gene            8039..10219
                     /gene="rnr"
                     /locus_tag="VGM72_01115"
     CDS             8039..10219
                     /gene="rnr"
                     /locus_tag="VGM72_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEY4263891.1"
                     /translation="MLARLATHPGETKRDLARALGVKGEERQILKRILTELAESGAIT
                     RGEKRSFVPAGALPEVAVLEIFGEDPDGEPLGRPLEWKGEGPPPSVLILPGREETSAP
                     GRGERVLARIARSKDGVTRYEARIIKKLGASAHRVLGVLKIERGMLARIEPIDRKTRS
                     AFVVDATELAGAKDGELVVAEPTVRHGASGPHARIVERLGSMNEPKTVSLIAIHAHGI
                     PTEFPLEALREAENAKPVSLAGRTDLRKIPLITIDPEDARDHDDAVWAGADPDPSNKG
                     GYIVVVAIADVAHYVRAGSALDREAEKRGNSVYFPDRVVPMLPEALSADLCSLRENEE
                     RACLAVRMVFDAHGAKRRHEFLRGLMRSAARLTYAQAQAAFDGRPDAKTAPLQAAVMQ
                     PLWDAYRAAVIARNERSPLDLDLPERRIVIGADGKIANIAFRERLESMRLIEEFMIMA
                     NVAAAESLEKARTPLIYRVHEEPSQEKLYAFADYLKTIGVSFAKGQVIKPAIFNRILA
                     RAKGTPHEEVMNDVVLRTQAQAVYAAENLGHFGLNLARYAHFTSPIRRYADLIVHRAL
                     IRGLKLGDGGLSDSDAARLKEIADHISMTERRAMAAERDSVDRYVAAFMEDRVGAEFD
                     GRVTGVTRFGLFLRLKETGADGLLPIRALGTEFFQHDEKRHALVGDRTGTLYRLGDII
                     RVRLAEAAPLTGGLRFDLAGGQAAGSRRPHRAGARPSLGRGKRR"
     gene            10488..10895
                     /locus_tag="VGM72_01120"
     CDS             10488..10895
                     /locus_tag="VGM72_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY4263892.1"
                     /translation="MARIFTSAVRAATASIAAAGFMFVAQSAQAAAAGDAKAGAAVFM
                     DNCGVCHTSGKGEAAKIGPNLFGVVGRKSASIAGYMYSPAMMKANLTWDVPTLQIYLM
                     GPQKKVPGTKMGFPGFSDPQDEADVIAYLVSLK"
     gene            11043..11621
                     /locus_tag="VGM72_01125"
     CDS             11043..11621
                     /locus_tag="VGM72_01125"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY4263893.1"
                     /translation="MQKFRTSLIAAATAMAMFAGGAYAASLDSLVIHGDAAKKIHEKY
                     ALSIDAAKKMAATCIAYAKSKNVAVSISIMDPIGEMIYFERMDGQGKLNTSTAILKSK
                     TALVTRASTHIVMNNVQQGQASEFRAGFFNGEFANKGGLPIIVEDQFLGAIGVGGSNV
                     DEECAQAALEAVVGPQPPLVQNLPRPGRANGG"
     gene            11762..11929
                     /gene="rpmG"
                     /locus_tag="VGM72_01130"
     CDS             11762..11929
                     /gene="rpmG"
                     /locus_tag="VGM72_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011454349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEY4263894.1"
                     /translation="MAKPTTVKIRLNSEANTGFFYVVKKNTRTMTEKMSVKKYDPVAR
                     KHVEFKEGKIK"
     gene            12053..13543
                     /locus_tag="VGM72_01135"
     CDS             12053..13543
                     /locus_tag="VGM72_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEY4263895.1"
                     /translation="MTIATDWRAEASRLAIRDKAFIGAEFVNAASGETFDCVSPVDGA
                     VLAKVAACDSVDVDRAVKAARAAFEDGRWAGQAPKARKRVLVKLAELIQKNANELALL
                     ETLDMGKPIANARNGDMRAVAECVRWYGEAIDKLYDEIAPTPKTALALITREPVGVAG
                     AVVPWNFPLLMAAWKFAPALAAGNCVVLKPAEQSPLTALRVAELAAEAGIPAGVFNVV
                     PGFGETAGQALGRHMDVDALAFTGSTEVGKLFLRYSAESNMKTVSLECGGKTPNIVFA
                     DTPDLDAAARGAADGIFYNSGQVCDAASRLLVEDKIHDAFLSKIAALASAYQPGDPLN
                     DKTQMGAMVDGTQTKRVLEFIGQGKAEGATLALGGKQVRQESGGFYIEPTIFDGVENR
                     MTIAREEIFGPVLSTIRFKTPEEAVQIANDSKYGLEANLWTNDLTKAHKIARALRAGT
                     VTINTRDGGGIEVPFGGYKQSGFGRDKSLHAFHKYTQLKTTYIHIR"
     gene            complement(13552..14481)
                     /locus_tag="VGM72_01140"
     CDS             complement(13552..14481)
                     /locus_tag="VGM72_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008417 - fucosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 10"
                     /protein_id="HEY4263896.1"
                     /translation="MKPQIRAAFAYFWPGFTPDHFRNFFPFVYDKYELVPSGNPEVVF
                     YSVFSPQFRAYADAREVHSVARLQPGNYQRVFMTGENFEPDMAACEFAMTFSSLADHP
                     NHLRLPLWVYEGRGWGFSPELLVKRVDMDWEKIAAEKTEFCNFVYLHEVPFRDGIFRS
                     LSTYKRVDSAGRHLNNMNGWMVPYMPSRVAAKIDFFRRYKFTLGIENDIWPGYMTEKL
                     VDPMFAHSIPIYVGDPQAKNSFDPASYIDFSCFANMKQMLEFVRAVDNDRDLHMKMLA
                     TPFYRDNTVPDYAREETILAFFDRIVDAALARR"
     gene            complement(14490..>14989)
                     /locus_tag="VGM72_01145"
     CDS             complement(14490..>14989)
                     /locus_tag="VGM72_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263897.1"
                     /translation="LLYPVFPVYFLQTPKEVVMIWAEDHQVRHVYLNQPHSPKPTLSW
                     FGESVGRYDGDTLVVDTVGLNGRTFVDNFRTPHTDRLHVVERFRMIDGGRTLEAKIHV
                     EDPGAFTMPWDAVQRYHRIDSASDKSDARIPHNGLEEMACAETSGDILNEGLESIPHA
                     DRPDF"
BASE COUNT         2904 a         4627 c         4538 g         2920 t
ORIGIN      
        1 tgctgcgggc gcggttgccg ccaggagcgc cgcaaagctg acacccaaca tcccgaacga
       61 ggccattctc ttcgccattt ctcatccctc aaaaatccga tgagggactg tagaccacag
      121 cgcggcgttg tacaaaaaat ctggcccggc aaaagaaaag gcggattcca ctgtcgtgga
      181 atccgccttc caaccaggac gcagccgaaa ctatttcgcc agcggatcgg gccgatactg
      241 cacgtggacg accttgcttt gctggccctt gccgccttca agatgcggct gcgagcggcc
      301 ggaatattgc gtccacaacc cccggccctt ccagccggtg gccatatcgt cgatgcgccc
      361 gtcgaggttc ttggcataga agctcagcgg ataaggaatg cgcatcacca tccacttgcc
      421 gttgatgagc gcgttcaacg aatccgaatt gttgccggtc acgatcggca cattttcgcc
      481 gagaccgaag gtgttgtgct gatccaccca gacgtaatag ggcgcttcgg cggatgcgcc
      541 gggcgtcccc ttcggcaggc tttcaaactc cggcccaggc atctggtaga gcttgaaacc
      601 ttcgggacaa agattctgcg gatcgttcgc cgccggaccg ttgagcttac ccttgcacag
      661 atgccggtcg aagctcgcca gatgaccgct cgaaagcacg gcccagaaca cgccgttgct
      721 gtcgatgtcg gagccgcgag ggctaaagcc cgactgcgga ttcttcgcgt tcttgtatgg
      781 gaactcgtaa tattccgtca gttgggtctt gggatcgaaa cgcgcgatac cgcccgggaa
      841 gcccgtcacg ccgccccaga tcgtgccgtc cgccggattg ggcgcaacgc cgtagaatcc
      901 caggaacatg cgcttatcct tggcgggatc ggcgggctgg tttggttcgg tccaactgac
      961 atcgcgcttg ccattgccat tggtgtcgat gacgaaaggc gaccagccct ggctcatctt
     1021 gtcgtcatgg gtggcttccc acttcttcac atcgagccag ccgataacat cgtaattgcc
     1081 gccggcgctg aaccacaaaa tgtcgttctt gtcgaattgc agatgatggg tgttgaagca
     1141 gagatcgatc atcgaaatct tccgcgtctt ggggtcgtac atctcgacct gacgattcga
     1201 tgtgttcttt ggatagatct tcgccgaggg ctgatcggac ccttccttgc agaaggccgg
     1261 attggcctgc ggacgaatgc gcgccgtcaa ccacacgcgg ccgttttcgt cgaacatggg
     1321 attgtggatg atcgtgtgcg aatcccagat cggctcattg ccccaatagg gcgagggcgc
     1381 caggatcaga tcggtcttgc tggtcggtgt cttgggatcg cggaattccg acttgatgaa
     1441 gcccgatttg ttgtccaccg gatcgagcca gggaacattg tcggagctga cttccggtgc
     1501 gccgtagatg agcccgtggc cattgacggt gggattgcgc cggtcggtcg aaatcgtatc
     1561 gtggacatac atggtgggcg tttcccaatc ccactccgtc agcacgacgt tgcgctccat
     1621 gcccacagga cgcggtgggt cgttcttggg cagttcgccc ttggcgacgc ggtccgtcca
     1681 atcaccgagg aaattcagtt gacgttgcac gtcgagcttg gcgagctctt ccatcatgtt
     1741 gcggccggcc tgtccggatt ccagacgccg ttcccaggct tccgcgctgg tgttgaaatg
     1801 gccgaggtcg gcgggaagcg tgcgcgtcgc cagatcgccc aattgatggc aatggccgca
     1861 gccgtccgtc ttggtgaagc gcagccattg cccctgatcc ttcagggtca ccgacatgcc
     1921 attgccttgc ggacctgttc ccgggaacag attcgcgggc gggatcttca tcatcgcgta
     1981 ccaataaatt cccggataat aggccgccgc cgccgcgcga ttcggcgccg ccacggattt
     2041 cagatcgacg agcttgccgg gcgtcgcttt caccttgggg gaatcgacca gaccgtaacc
     2101 gcggacccag atatcgtagg tgcccttcgg caggtcgggg atcacatagc gcccctgatc
     2161 gtctgtagcg acgatcttga cgtatttggt cggaagatcg cgcgtctccg cgatgaccca
     2221 cacgccggct tccggcccct tggcgccagt gaccacgccg ccgatgtcgt cagcgtcgat
     2281 cttgacctgc tccgcgggtt gcgccacggc ggtcgtcgcg gaagccgcgc acaacagcgc
     2341 cacgaaactg gcgctcatca tgccgaggca ggctgctctc ttcgtcctca ttttttcttc
     2401 cctcatagcg ttaagaatga cgctgaatat ctatggcgga ttgcagcgaa gaatgagccg
     2461 gttccagcca tccggaacac tacgcaaaaa tgaaggcgga ttccactctc gcggaacccg
     2521 ccttcggctt aactgatctt aatccgcgtt atttcgcgag cggatccggc cgcagttgga
     2581 agtggacaac cttgctcatc tggcctttgc cgccttcgat gtgcgtggcg gcgcggtcgg
     2641 agaaggtgga ccataggccc ttgcccttcc agcccgtggc ggcatcgtcg atgcgcccgt
     2701 ccataccctt ggcgaaatag ccgatcggat aaggcacccg catcaccacc catttgccat
     2761 tgacgatcgc cgccagcgaa tcggactcat tgccggtggc gatgggggtg ttcttaccga
     2821 ggccgagcgt gtcgtattga tcgacccagt tgtaataggg cgcttccgtg cccgcaccgg
     2881 gtgtgccctt gggcaggcct tcgaactccg gcccaggcat ctgatagagt ttccacccct
     2941 cggggcacag attctgcggg tcggccaaag ccggtccgga gagcttgccc ttgcacagcc
     3001 tgcgatcgaa gctggcgaaa tggccgctcg ccagaacggt ccagaccaca ccatcgctgg
     3061 taatgtcggc accgcgcggg ctgaagccca tcgtctcggc cttcgcattc ttccaaggca
     3121 cttcgaaata ttcggtgagc tgcgtcttcg gatcgaagcg cgcaacaccg cccgggaagc
     3181 ccagaacggt gccccagaca gaaccgtcgg ccggattcgg agacacgctg tagagaccgg
     3241 tgacaatgcg catgtccttc gtcggatcga gcttgccttt gggatcgacc cagccggcat
     3301 cgcgcttgcc gttgccgttg gtgtcgatga caaagggcga ccagccctgg ctcgcctgct
     3361 cgtcatgagt ggcgtcgaat ttcttcacgt cgagccagcc gatgacttca ttgttgccgc
     3421 cgcccgagct gaaccacagc atgcccgtct tgtcgaattg cagatgatgc gtcgagaagc
     3481 agagatcgac catggtgatc ttgcgggtct tgggatcgaa catctccacc tgacgtccgg
     3541 agcgcccttg cgggaaaatc ttcgcggagg cctgctgcga accttctttg cagaaggccg
     3601 gattgttctg cggacgaatc cgcgccgtca gccacagccg gccctggctg tcatacatcg
     3661 ggttgtggac attggtgtgg ctgtcccaga tcggctcgtt cccccaatag ggcgactcgc
     3721 catagatcgc atcgttcttg gtggtgggcg tcttgggatc gcggtattcg gtcttcagaa
     3781 ggcccgcttt gttgtccacc ggatcgagcc acggaatgta atccgaactc gcttcggtcg
     3841 aaccgtaaat gatgccgtgg gcattcaccc cagggttgtt gcggtcggtc gagatctcat
     3901 cgtgcaaata ggctttgggc gtattccagt cccattccgt cacgacgatg tttctctcca
     3961 ggccggcggg acgcggcgga tcggacttgg gcaattcgcc cttcgagacg cggtcggtcc
     4021 aatcggcgaa catcgacagc gcgcgctgcg tgtcgaagcg gccgatggcg cccatcatgg
     4081 ggccgctggc ctgtccggac tgcagccggc gttcccatgc ggccgccccc gaatcgaagt
     4141 gcccgagcgc agcgggaatg gtgcgtgtcg ccttgtcgcc aagctgatgg caggcgacgc
     4201 atccgtcggt cttcatgttg cgcagccaca caccctgatc gctcatctgc tgcgacatgt
     4261 tgttgcctgc ggggcctgtg ccggggaaca gattcgatgc cggcggcttg accatcgaat
     4321 accaatattg cgccggataa tattccgccg ccgccgcacg gctcggtgcc gccacagcct
     4381 tgaggtcgag taccttgccg ggcgccgatt tcactttggg cgaatcgacc agcccatagc
     4441 cgcgaaccca gatgtcgtaa ttgcccttgg gcagatcggg cagcatgtag cggccggaat
     4501 cgtcggtcac gacgatcttc acatatttgg tgggcagatc cttggtctcg gcgatgaccc
     4561 agaccccagc ctccgggccc ttggcgccgg tgacgacgcc gccgatatcg gaggcgtcca
     4621 cacgcacctg ttgcgccgtc gcgcttgtcg ccgacacggc aaagagcaat gccgcgacgc
     4681 tcgtactcag catgccgata ttggctgtcc tggtcctcat gtgtccgtcc ctcgtggcgt
     4741 taggattgaa ttctcagcga gcaaaatgaa gtgcgctctt atagcgcatc ccggaacaaa
     4801 ggacgagagg cgccgcccca cgaatgcgtg cgcaaaaccc gcgacgcgcg atcgcttgac
     4861 cccaaccgta cggtgctgta cagtcttggc atggatgatt atcctcgtaa gggtgcggag
     4921 gaagctcgca accagcttcc ggaccttctc gatgccgccg aaaatggcaa aaccaccatc
     4981 atcacccgtc atggccggcc ggttgccgcc ctcacgcctg tggatgtcct gagcgctgct
     5041 cgagagcagc aacctctgac tcctctgaag ggttcgggca ccggcttgtg gggaaagaac
     5101 agcacccaca cccttcgggc gctgcgggac gaatggcaca attagactta gcggacttgc
     5161 cggccggagc actgctcctg atcgattcag ctcccgtgat ctacttcatg gaggcgcacc
     5221 cgaaatgggc ctctcgcttc agacccgttt tcgaggcgca tgccgcaggt cggtttcgct
     5281 ttgccgttac aaccatcacg atcgctgaag tcctgatcgg tccatttaag gcgggagatg
     5341 aggttctcgg ccgtcgttac cgttcggtcc ttgaatcgtg gcaacgcatc gaccttgatc
     5401 tggatatcgc ggatagcgca gcgagattgc gtgcgtcgct cggcttcaaa ttgcccgatg
     5461 caattcaggt ggcaagcgcg ctcaaaataa atgctgctgc cctcgtgact cacgatcgcg
     5521 acttttcgcg cgtgcgttcg ctacgcgtga tttcctgaga ggccaaaaca aaaggcgggt
     5581 ccgctttcgc gaacccgccc ctgaaaatct ccctagaccg tcagcccagg gttaacgatc
     5641 ctaatccgcc aagggattag gacggacctg aaaatgcacg accttgctgg tctggccctt
     5701 gccgccttcg atatgcggcg cggagcggcc agagtaggtc gagaagatgc ccttgccctt
     5761 ccagccggtc gaggagtcgt cgatgcgtcc gtccatgttc ttcgcgaaga agcccatggg
     5821 atacggcacg cgcatggtga cccatttctc gttgaccagt gcgaacagcg catcggaaag
     5881 attacccgtg gcgatgggca cgttcttgcc caggcccaga gtatcgaact ggtcgaccca
     5941 atcgtaatac ggcgcttcgg cgctaccgtc cgtcaccgtt atgcccttaa attgcgggcc
     6001 ggggatggtg tagaacttcc agccttcggg acaggctttt ccggtcgccg cgatcggccc
     6061 gttcaatgga cctttgcaaa gcttgcggtc gaagctcgcc atgtgaccgc tcgccagcgc
     6121 cagccacgca acgccgttgc tgtcgatgtc catgccgcgc ggagagaaac ccgaacgggg
     6181 gttcttgggg tccttgtagg gaacctcata gaattccgtc agctttgtct tgggatcgaa
     6241 gcgcgcgacg ccgccgggga acccgagata ggacgcatac accgtgccgt ccgccggatt
     6301 gggcgacgcg ccgtagaagc caacctggac atgcttgtct ttcttgggat cgacagcatc
     6361 cttcggaccg acatactcgt cgagcttgcc attgccattg gtgtcgacaa cgaacggcgc
     6421 ccagccttgg ctcgcctgtt cgtcatgggt ggcgtcccat ttcttcacgt cgaaccaacc
     6481 gacaacctcg tcattggtgt tgccgctgga gaaccacatg acgttgttct tgtcgagctg
     6541 cagatggtgc gtggagaagc acagatcgat cggcgtcgcc ttcttggtct gcgggtcata
     6601 aacttcgagc tggcgacctg aacggctcag cgggaatgcc ttcgccgagg gatggttcga
     6661 tccggctttg cagaaggcag gctccgcgtt cgccggacga atgcgcgccg tcacccacag
     6721 gcggctcttt tcgtccatcc agggattgtg gatgacggta tggctgtccc agatcgcatc
     6781 cgtgccccaa tagggcgatt ccccatattg cggcgcggtc ttggtggtcg gcgtcttggg
     6841 atcgcgatat tcggatttga tcagacccga tttgttgtcg acgggatcga gccaaggaat
     6901 gaagtccgaa ctttcctccg gcgaaccata gatcaggccg tgcggattga gagtgggatt
     6961 gcggcggtcg gtcgaaatct cgtcatgaag atagctctgc ggcgtcgccc aatcccattc
     7021 ggtgagcacg acattgcgct cgatgccggt gggacgcgtc ggcttcgcga agggcagttc
     7081 gcccttcttc acgcggtccg tccaatcgcc caaattcgcc agcgcgcgcg cggtgtcgaa
     7141 attgccgatg ccgttcatca tcaaggcgct cgcctgtccc gattggagac ggcgttccca
     7201 ggcggcagcc gaggttgcga aatgcccgag ttgcggctcg agttcacgcg tcgccttgtc
     7261 gccgatctga tggcaggaat tgcagctgtc ggttttcatg tagcgcagcc acatgccctg
     7321 atcggtgagc gagacgggca tgccgttgcc cgaaggaccg gtaccgggga agagtttggg
     7381 ctcggggact ttcaccatcg agtaccaata gatcgccgga taatattccg ctgcagcggt
     7441 ttcgttcggg gccgccgtcg atttcagatt gacggtcttt ccgagcgcgg tcttggtgcg
     7501 cgcggagtcg acaaggccgt aaccgcgcac gaacacatcg taattcgctc tgggcaggct
     7561 ggggatcaaa taacggccgg aatcgtcggt cacgacgatc ttcacatatt tggtcggcag
     7621 atccttggtc tccgcgatca cccacacgcc ggcctcggga ccttttgcgc cggtgaccac
     7681 gccgccgatc tcatcggctt tgacttgcac ggcccccccg gctggttgcg cgctcgcagg
     7741 cagcggcgcg gcgataaaca acgcggtgcc cataaggctc gccgccaaca tgctcaaaca
     7801 cgacctaccc gcggtgctca tgccccgtat tcctccaatg cagcttgcag ggattggcag
     7861 atttaccagt cctctgccag tttagggcaa atgcggacag cggcaaagcc gatcctgtcc
     7921 gcgacgcgca aataagcttt atgttcaagc atccgtgaac ttgaggaata tcttggcgaa
     7981 accgccccga cgagtccccc gcccgcgccc cccgaagact ggcctgtcca aggaaaacgt
     8041 gctggcccgc ttggccaccc atcccgggga gaccaagcgc gacctcgccc gcgcgcttgg
     8101 cgtaaaaggc gaggaacgcc aaatccttaa gcggattctc acggagcttg ccgagagcgg
     8161 tgcgatcaca cgcggcgaaa agcgcagctt cgtaccggcc ggcgcccttc ccgaagtcgc
     8221 cgtgctggaa atcttcggcg aggacccgga cggagagccc cttggacgcc cgctggagtg
     8281 gaaaggcgaa ggccctcccc cctcggtcct gatcctcccg ggccgcgaag aaacctccgc
     8341 accgggccgc ggcgaacggg tgctggcgcg aatcgccagg agcaaggatg gcgtcacgcg
     8401 ctacgaggcg cgcatcatca agaagctcgg cgccagcgcc caccgtgtgc tgggtgtgct
     8461 caaaatcgaa cgcggcatgc tggcgcgcat cgaacccatc gaccgcaaga cgcgctccgc
     8521 cttcgtcgtc gatgccacgg aattggcagg cgccaaggac ggagaattgg tggtcgccga
     8581 acccacagtg cgccacggcg cgagcgggcc gcacgcgcgc atcgtcgaac ggctgggttc
     8641 gatgaacgag cccaagaccg tcagcctgat cgcgatccat gcgcatggca ttccgacgga
     8701 gtttcctctc gaagcgctgc gagaggcgga aaacgccaaa cccgtcagcc tcgcgggccg
     8761 cacggatttg cgaaaaattc cgctcatcac catcgaccct gaagatgcgc gcgatcacga
     8821 cgacgcggtg tgggcgggcg cggatcccga tccctccaac aaaggcggct acatcgtcgt
     8881 cgtcgccatc gccgatgtcg cgcattatgt gcgcgccggt tcggcgctcg accgcgaagc
     8941 ggaaaagcgc ggcaattccg tctatttccc cgaccgcgtc gtccccatgc tgcccgaggc
     9001 gctgtcggcc gatctgtgct cgctgcgcga aaacgaagaa cgcgcctgtc tcgccgtgcg
     9061 catggtgttc gatgcgcatg gcgcaaaacg ccgtcacgaa ttcctacgcg ggttgatgcg
     9121 ctcggcggcc cggctgacct atgcgcaggc gcaagccgcc ttcgacggca gacccgatgc
     9181 gaagaccgcc cccctgcaag ccgcggtcat gcagcccttg tgggatgcct accgcgcggc
     9241 tgtgatcgcg cgcaatgaac gctcgcccct cgatctcgat cttccggagc ggcgcatcgt
     9301 gatcggcgcg gacggcaaaa tcgcaaacat cgcgtttcgc gagcgcctgg aatccatgcg
     9361 gctgatcgaa gagttcatga tcatggccaa tgtcgcggct gccgaatcgc tggaaaaggc
     9421 gcgcacgccc ctgatctatc gggtccatga agagccgagc caggaaaagc tttacgcctt
     9481 cgccgattat ctgaagacca tcggggtctc ctttgccaag ggccaggtga tcaagcccgc
     9541 catcttcaac cgcatcctcg cgcgcgccaa aggcacgccg catgaggaag tgatgaacga
     9601 tgtcgttttg cgcacgcagg cccaggcggt ctacgcagcc gagaaccttg gacatttcgg
     9661 attgaacctg gcgcgttatg cgcatttcac ttcgcccatc cgccgctatg ccgatctcat
     9721 cgtgcatcgc gcgctgatcc gcggactgaa gctcggcgat ggcgggctct ccgattccga
     9781 tgccgcgcgg ctcaaggaaa tcgccgacca catttcgatg accgaacgcc gcgccatggc
     9841 ggccgaacgc gattccgtcg accgctatgt cgcggccttc atggaagatc gtgtcggcgc
     9901 ggagttcgac ggccgggtga ccggcgtgac gcgcttcggc ctgtttctgc ggctgaagga
     9961 aaccggcgcc gatggattgc tgcccatccg cgcgcttggc acggaattct tccaacatga
    10021 cgagaagcgg catgcgctcg tgggcgaccg cacgggaacg ctttatcggc tgggcgatat
    10081 catccgtgta cgccttgccg aagccgcccc cctgacgggg ggattgcgct tcgatctggc
    10141 gggcggacaa gcggcaggct cccgcagacc ccaccgcgcc ggtgcacgtc cgagcttggg
    10201 ccgcggcaag cggcgctgat cgttccggtc attcccggcc cggcggggtt ttcgattcgg
    10261 cacaccggcg caggaccgat aaggcacagc gccccaggtg cgcgcgaagg tcgcagccgc
    10321 caacgcaccg gccggccagc gtttttcctg gaatttccgc gagttggccg gctcggccgg
    10381 gggaggacga cggggcgcct tcaatttgcc accagaccca tgttttatgt tatgcgttag
    10441 atgcatgacc gatcggtcat agagaaacgc aaatcgaggg agttcccgtg gctcggattt
    10501 tcacatcggc ggtgcgcgcc gcaacggcca gcatcgcagc agccggtttc atgtttgtag
    10561 ctcagtccgc tcaggcggcc gcggctggtg acgccaaggc tggcgcagcc gtgtttatgg
    10621 acaattgcgg cgtttgccac acgtcgggca agggcgaagc cgccaagatc gggccgaacc
    10681 tgttcggcgt cgtcgggcgc aaatccgcaa gcatcgccgg ttacatgtat tcgccggcaa
    10741 tgatgaaggc gaacctcacc tgggacgtgc cgaccctgca gatctatctg atgggcccgc
    10801 agaagaaagt gccgggcacc aagatgggct tccccggctt ttccgatcct caggacgagg
    10861 cggacgtcat cgcctatctc gtcagcctga agtaattccg ccgttttgaa actgccgtca
    10921 tagccactgc gcccgcggat ggccgcgatg gcagtttcat tttcaggtaa gcctctcgca
    10981 attgaagact gcccaggagg gagcacactc cctctcctat cgggcatcag cgggaggaac
    11041 ccatgcagaa attccggaca agtttgatcg cagcggccac ggccatggcc atgttcgcgg
    11101 gcggcgcata tgccgcttcg ctcgatagcc tcgtcatcca tggcgatgcc gccaagaaga
    11161 tccacgagaa atacgccctc tccatcgacg cggccaagaa gatggccgcg acctgcatcg
    11221 cgtacgccaa gtccaagaat gtcgccgtct cgatctcgat catggatccg atcggcgaga
    11281 tgatctattt cgagcgcatg gacggccagg gcaagctcaa cacctcgacg gccatcctga
    11341 agtcaaaaac cgctctcgtg acccgcgcct ccacccacat cgtgatgaac aatgtgcagc
    11401 agggacaggc cagcgagttc cgcgccgggt tcttcaacgg tgaattcgcc aacaaaggcg
    11461 gcttgccgat cattgtcgag gaccaattcc tgggcgccat cggtgtcggc gggtccaatg
    11521 tcgatgagga atgtgcccag gccgctctcg aagcggtggt gggaccccag ccgccgctgg
    11581 tgcagaacct gccccggccc ggccgcgcga acggcggctg atccttcggc aagctcagga
    11641 ggaggagggc attcctcatg gtgagcctgt cgaaccatgc cccgatattg gccatgtctt
    11701 gacatgagag ggcggctgag taggttagcc gcccttcacg tttcgttgcc caggagttcc
    11761 tatggccaag ccaaccactg tcaagatccg gctgaacagc gaggccaaca cgggcttttt
    11821 ctacgtggtg aagaagaata cgcgcaccat gaccgagaag atgtcggtca agaaatacga
    11881 tccggtggcg cgcaagcatg tcgagttcaa ggaaggcaaa atcaaatagc gcaacgcctg
    11941 cgcctggcct gcctgccctg cttttttcca cctgcccgat ctctcgcttt tgcgtgcctc
    12001 cgtaagggcc gtttgccgtt atgatgcgtg cacgcgcatt tgagaggcag agatgaccat
    12061 cgcaacagac tggcgggccg aagcttcccg cctcgccatc cgcgacaagg cgttcatcgg
    12121 cgcagaattc gtcaatgccg cgagtgggga gaccttcgac tgcgtctcgc cggtggatgg
    12181 cgcggttctc gccaaggtgg cggcctgcga ttcggtggac gtggatcggg ccgtgaaggc
    12241 cgcccgcgcc gcgttcgaag acggacgctg ggccggccaa gcgcccaagg cgcgcaagcg
    12301 cgtgctcgtg aagctcgccg aactgatcca gaaaaacgcc aacgagctgg cgctgctcga
    12361 aaccctcgac atgggaaagc cgattgcgaa tgcccgcaat ggcgacatgc gggcggtggc
    12421 cgaatgcgtg cgctggtatg gcgaggccat cgacaagctc tatgacgaga tcgcgccgac
    12481 accgaaaaca gcgctcgccc tcatcacgcg cgaacccgta ggcgtcgcgg gcgcggtggt
    12541 gccgtggaat tttcccctgc tgatggcggc atggaaattc gcgcccgctc tggccgctgg
    12601 aaattgcgtc gtgctgaagc ccgccgaaca gtcgcccctg acggcgctgc gcgtggcgga
    12661 actggcggcc gaagccggca tacccgccgg tgtgttcaac gtcgtgccgg ggttcggcga
    12721 aaccgctggg caggcgctcg gccggcacat ggacgtggat gcgctcgcct tcaccggctc
    12781 gaccgaggtg gggaagctgt tcctgcgcta ttcggccgag tccaacatga agaccgtcag
    12841 cctggaatgt ggcggcaaga cgcccaacat tgtgttcgcg gatacgcccg atctggacgc
    12901 ggcggcacgt ggggcggccg acggcatttt ctacaattcg ggccaggtgt gcgacgcggc
    12961 ttcgcggctt cttgtcgaag acaaaatcca cgatgcgttc ctatcgaaga tcgccgcgct
    13021 cgcgagcgcg tatcagccgg gcgatccgct gaacgacaag acccagatgg gcgctatggt
    13081 agatgggacg cagaccaaac gcgtgctcga attcatcgga cagggcaagg ctgaaggcgc
    13141 gacactcgcg ctcggcggaa aacaggtgcg ccaggagagc ggcggcttct acatcgagcc
    13201 cacgattttc gacggcgtcg aaaatcgcat gaccatcgcg cgtgaagaaa tcttcggccc
    13261 cgtgctatcg acgatccgtt tcaagacgcc ggaagaggcg gtgcaaatcg ccaatgattc
    13321 aaaatacggc ctggaagcca atctgtggac caacgatctg accaaagcgc ataaaattgc
    13381 gcgtgccctt cgtgcgggca ccgtcaccat caacacacgc gatggcgggg ggatcgaggt
    13441 gccgttcggc ggctacaaac aatccggctt cggacgagac aaatcgctcc acgcgtttca
    13501 caaatacacc cagctcaaga cgacctatat ccacatcaga tgagcagccc attatcgccg
    13561 ggccagagcc gcatccacga tacggtcgaa gaaggcgagg atcgtttcct cgcgcgcgta
    13621 atcgggaacg gtattgtcgc gatagaatgg cgtcgccagc atcttcatgt gcaggtcgcg
    13681 gtcattgtcc accgcgcgca cgaattccag catctgcttc atgttcgcaa aacaactgaa
    13741 atcgatatag ctcgcgggat cgaaggaatt tttcgcctgc ggatcgccga catagatcgg
    13801 aatggaatgc gcgaacatcg gatcgaccag cttctcggtc atatagccgg gccagatgtc
    13861 gttctcgatg ccaagggtga atttgtagcg gcggaagaaa tcgatcttcg ccgcgacccg
    13921 gctgggcata tagggcacca tccagccatt catgttgttg agatgacggc ccgcggaatc
    13981 gacgcgctta taggtgctta ggctgcggaa gatgccatcg cgaaacggca cctcgtgcag
    14041 atagacgaaa ttgcagaatt cggtcttctc ggcggcgatt ttttcccaat ccatatcgac
    14101 gcgtttcacc aatagctccg gcgaaaaacc ccaccccctg ccctcataga cccacagcgg
    14161 caaacgcaga tgattgggat gatcggcgag cgacgaaaac gtcatggcga attcgcacgc
    14221 cgccatgtcg ggctcgaaat tctcccccgt cataaacacg cgctgataat ttccgggttg
    14281 cagccgcgcg accgaatgaa cctcgcgcgc gtccgcatag gcgcggaact gcggcgagaa
    14341 cacggaatag aacacgacct ccggattgcc cgacggcacc agctcatatt tgtcatagac
    14401 gaaaggaaag aaattccgga aatgatcggg cgtgaatccg ggccagaaat aggcgaaggc
    14461 cgcgcgaatt tgaggtttca tcgaaggttt cagaaatccg gccggtccgc gtgcggaatg
    14521 gactcgagac cctcgttcaa gatatcgccg ctggtttcgg cgcaggccat ttcttcgaga
    14581 ccgttatggg gtatgcgcgc atcggatttg tcgctcgccg aatcgatgcg gtgataacgc
    14641 tgaaccgcgt cccacggcat cgtgaacgcg ccggggtctt ccacatgtat tttggcctcc
    14701 agcgtcctgc cgccatcgat catgcgaaag cgctccacca catggagtct gtcggtgtgt
    14761 ggcgtgcgga aattgtcgac gaaggtccgc ccgttcaacc ccactgtgtc gacaaccagc
    14821 gtgtcgccgt catagcggcc aaccgattcc ccgaaccagg agagcgttgg cttcggcgaa
    14881 tgcggctggt tcagatagac atgccgcacc tgatgatcct cggcccagat catgaccacc
    14941 tccttgggcg tctggaggaa gtagaccgga aagaccggat acagcagcc
//
LOCUS       DASYEG010000027         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_299803, whole genome shotgun sequence.
ACCESSION   DASYEG010000027 DASYEG010000000
VERSION     DASYEG010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299803"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..956
                     /locus_tag="VGM72_01150"
     CDS             21..956
                     /locus_tag="VGM72_01150"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY4263898.1"
                     /translation="MRGTQFAELSAFVAVAEQKSFTRAAQTLGISTATLSQAIRSLEE
                     SLGVRLLHRTTRSVGLTAVGERLLSRLRPVLDDYEGALESINDFRDRPCGLLRLTVLP
                     PASEFVLAPVLGRFLAEYRDIKLEILAEPALQGIVAERFDAGIRSGQRVERDMIAVRV
                     SPDIRIVTLASPAYLAQHGTPARPQDLRDHHCIRFRFPAGALMPWRFEKKGKRLEVAV
                     DGPLITSDPRLGVRVAADDGGVFYIAELYARELIAEGKLIAILDDWAPPPDAFYLYYP
                     SRRQNPAALQVLIEFLKGNLRDGGARHERRLSVKT"
     gene            complement(953..>1501)
                     /locus_tag="VGM72_01155"
     CDS             complement(953..>1501)
                     /locus_tag="VGM72_01155"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HEY4263899.1"
                     /translation="HEEMHSDAFTRSGGPFEAIQLWVNLPSRLKMTTPKYQTLLAAEI
                     PDVALPDNAGRVRVIAGRFGTAKGPADTFTPMNLWDVRLNAGHGGSFTTQAGHTGAVF
                     VLKGIVTVNGAKARESEFVLLDRKGEAFRIEAETDATLLVLSGEPIDEPVFGYGPFVM
                     NNEAEIRRAMADYQSGKMGHLA"
BASE COUNT          265 a          499 c          443 g          294 t
ORIGIN      
        1 tttttcgatg agatcaaaca atgcgcggca cccaattcgc cgaattgtcg gccttcgttg
       61 cggtcgccga acagaagagc tttacgcgtg cggcccaaac gctcggcatt tccaccgcca
      121 cgctgagcca ggcgattcgc agtctcgaag agagcctcgg cgtgaggttg ctccatcgca
      181 ccacacgcag cgttgggctc accgccgtcg gcgaacggct gctctcgcgt ctgcgtccgg
      241 tgctcgacga ttacgaaggc gcgctcgaat ccatcaatga ttttcgcgat cgcccgtgcg
      301 gtcttctgcg cctcaccgtg ctgcctccgg catcggaatt cgtgctggcg ccggtgctgg
      361 ggcgattcct cgcggaatat cgcgacatca agctggaaat cctcgccgag cccgccttgc
      421 agggtatcgt cgcggagcgt ttcgatgccg gcatccgcag cggtcaacgt gtcgaacgcg
      481 acatgatcgc agtgcgcgtg agccccgata tccgtatcgt cacgctggcg tccccggcct
      541 atctggcgca gcacggcacg ccggcgcgcc cccaggatct gcgcgatcac cactgcatcc
      601 gcttccgctt tccggccggt gctctgatgc cttggcgctt cgagaaaaag ggcaagcgcc
      661 tggaagtggc ggtcgatggt cccctcatca ccagcgaccc acgcctcggc gtgagagtcg
      721 cggccgacga tggcggcgtg ttctacatag ccgaacttta cgcgcgcgaa ctgattgcgg
      781 agggaaaact catcgccatt ctggacgatt gggcaccgcc gcccgatgca ttctatctgt
      841 attatcccag ccgccggcag aaccccgccg cgctgcaggt gctaatcgaa ttcctgaagg
      901 gcaatttgcg cgacggcggc gcccgtcacg agcgccgcct atccgtaaaa acttaggcca
      961 gatggcccat cttgccgctc tgataatccg ccatggcgcg gcggatttcg gcttcattgt
     1021 tcatgacgaa cggaccataa ccgaacaccg gctcgtcgat gggctcgccg gaaaggacga
     1081 gcagcgtcgc gtccgtctcc gcctcgatgc ggaacgcctc gcctttgcgg tcgagcaaca
     1141 cgaattcgct ctcgcgcgct ttggcgccgt tcaccgtcac tatgcctttc agcacgaaca
     1201 ccgcaccggt atgtccggcc tgtgtcgtga atgagccgcc gtgcccggca ttcagccgca
     1261 catcccacag attcatcggc gtgaatgtgt cggctgggcc ctttgcggtg ccgaaccgac
     1321 ctgcaatcac gcgcacgcgg cccgcattgt cgggaagcgc cacatccgga atctcggccg
     1381 caagcagcgt ctgatatttc ggcgtggtca tcttcagccg ggatggcaga ttgacccaaa
     1441 gctggatggc ttcgaacggc ccgccactcc gggtgaaggc gtcggaatgc atttcctcat
     1501 g
//
LOCUS       DASYEG010000028         6311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_5083178, whole genome shotgun sequence.
ACCESSION   DASYEG010000028 DASYEG010000000
VERSION     DASYEG010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5083178"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VGM72_01160"
     CDS             <1..589
                     /locus_tag="VGM72_01160"
                     /inference="COORDINATES: protein motif:HMM:NF019944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HEY4263900.1"
                     /translation="ANGQRAYAPRSTDDMGKITALVRSAIGFDEKRGDQLQVTNMRFA
                     DIDTGPEAPVAQPFLGLDPPAIFKLAQVLILSITALLVFLLVVRPMIRRLTTPIAASG
                     PGGAAALPAPGASGAPQLAHGGTDAAGAQQQPAQIANANGPQPIAIPKRESMIDISQI
                     DGQVRESAIRKVGDVVQSHPEEAMAILRTWLHQPV"
     gene            601..1635
                     /gene="fliG"
                     /locus_tag="VGM72_01165"
     CDS             601..1635
                     /gene="fliG"
                     /locus_tag="VGM72_01165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEY4263901.1"
                     /translation="MARAPKTQVKEDIRALSGPERAAIFLLSLGEEHSTKLWQMMDED
                     EIKEVSQLMSNLGTVSSSLVEKLLIDFVSQMSSTGSLMGSYESTERLLARILPGEKVG
                     SIMEEIRGPAGRTMWDKLGNVNESVLANYLKNEYPQTVAVVLSKIKSEHAARVLGALP
                     EEFALEVVQRMLRMESVQKDILDKVEQTLRVEFMSNLARTAKRDAHEMMAEIFNNFDR
                     QTENRFLTSLEERSRDSAERIKALMFTFEDLGKLDPGSIQTMLRHVEKDKLALALKGA
                     TDSLRDVFFSNMSERAGKILREDMEALGPVRLKDVDEAQMRMVNVAKDLANKGEIMIA
                     SKQGEDELIY"
     gene            1635..2261
                     /locus_tag="VGM72_01170"
     CDS             1635..2261
                     /locus_tag="VGM72_01170"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HEY4263902.1"
                     /translation="MGTAVKFTFDTDFDSGAPDTARADARSRKNYSAEEIEEIKREAR
                     DEGHRDGNVRATQAVAASIGQVAAAVLSAIEAMDSEIEAIRSEAAGLAVAASKKLARA
                     ALAAAPEAEIAEALQVALHQAIGEPRVVIKTSPLLAQKIQERANEIANAEGYEGRMQF
                     VGDGGLSDADCRIEWRGGGIERTQAALEHALNDLVARRFARAPAEENE"
     gene            2261..2620
                     /gene="fliN"
                     /locus_tag="VGM72_01175"
     CDS             2261..2620
                     /gene="fliN"
                     /locus_tag="VGM72_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEY4263903.1"
                     /translation="MADKDDVGLPELGDDHGAQAGQPLLEKQSGGDADLKRAAIDLEA
                     VYDVPVTVQAILGKSAMEVSQLLKLGRGTVVQLDRKVGEAIDIYVNDRLVARGEVVLV
                     EDRLGVTMTEIIKSGQI"
     gene            2641..4014
                     /locus_tag="VGM72_01180"
     CDS             2641..4014
                     /locus_tag="VGM72_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY4263904.1"
                     /translation="MRLLIIGSLGGQIGAATRIALDKGAKVAHTNGVDQGLSELRQGR
                     GADLIMCDVALDIGLLVRSLAAERICTPVIACGVGTDARRAVDAIRAGAKEYLPLPPD
                     PELIAAVLAAVADESHQLIFEDEATASVIAMADQIAPSDASILVTGESGTGKEILARY
                     VHRKSARADKPFISVNCAAIPENLLESELFGHEKGAFTGAIARRVGKFEEADGGTLLL
                     DEISEMDARLQAKLLRAIQEREIDRVGGGKPVRVNIRIIATSNRDLSDAVKHGTFRED
                     LLYRLNVVNLRLPSLRERPRDILALARHFGRKYAEANGVPFRPLGGETERMLLGHNWR
                     GNVRELENTIHRAVLLSHGSEIMPEAIRLPDGGKLTEGPVRSGSDAVAIATEAAAAAT
                     TRALVGRTVSDVERDLILDTLDHCLGNRTHAANILGISIRTLRNKLREYTQSGIRVTQ
                     PGEGRAA"
     gene            4037..6145
                     /gene="flhA"
                     /locus_tag="VGM72_01185"
     CDS             4037..6145
                     /gene="flhA"
                     /locus_tag="VGM72_01185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEY4263905.1"
                     /translation="MSVQAQTFQSGPRGAFDAVRDYLRNGEIALAFGILAILVVLLLP
                     LPSWLLDLALSFSITFSILILMTSIFIRKPLEFSSFPTVLLISTMLRVALNLASTRLI
                     LAHGKEGTDAAGFVIQAFGRLITQGNFVIGIIVFAILIIVNFVVITKGAGRIAEVAAR
                     FTLDAMPGKQMAVDADLSAGLINETEARERRKSLEAESNFFGAMDGASKFVRGDAIAG
                     LLVVGINVIGGIIIGVAQQGMSFTDASQTFTLLTIGDGLVTQVPALIVSTAAGMMVSK
                     GGTEGSSEKALLGQLSFYPQALAMASFVMAVVSMLPGMPHVVFALIALGTGGLAYAAW
                     RNKETIRHDAAQSEAVKASEAPPVEEPISTALALDLLRVELGYGLLPLINDAKGHRVT
                     DQIKALRRQLASEMGFVMPAVRILDNMQLSANEYVVRVKEVEAGRGELYPGQLLLMDP
                     RGQAIELPGTHTTEPAFGLPATWVEQGLREEASFRGYTVVDPGTVLTTHLTEVLKTHM
                     SELLSYAETKKLLDDLPPEQKKLVEELIPSQIPVTGVQRVLQTLLSERVSIRDLPAIL
                     EGIAEALGHTKNAMLITEHVRARLGRQLCHQHLSAQGVLPIIAMSPAWEQTFAESLAG
                     TGEERQLAMAPSQLQAFIQTVRERFDDASSRGDLPVLLTSPLIRPYVRSIIERFRPQT
                     PVISQNEIHPSVRLRTMGQI"
BASE COUNT         1235 a         2049 c         1933 g         1094 t
ORIGIN      
        1 cgccaacgga cagcgcgcct atgcgccgcg cagcaccgac gacatgggca agatcaccgc
       61 actcgtacgc tcggccatcg gcttcgacga aaaacgcggc gaccagctcc aggtcacgaa
      121 catgcgcttt gcggacatcg acaccggccc ggaagcgccg gtggcccaac cgttcctggg
      181 gctcgacccg ccggccatct tcaagctcgc acaggttctc atcctctcca tcacggcact
      241 tctcgtgttc ctgctggtgg tgcgcccgat gatccgccgc ctcacgacac ccatcgcggc
      301 gagcggcccc ggcggcgcgg cggcgcttcc cgcgcccggc gcgtccggtg ccccgcaatt
      361 ggcgcatggc ggaacagatg ccgcgggcgc ccagcaacag ccagctcaga tcgccaatgc
      421 aaatggacca caaccgattg cgatcccgaa acgcgaaagc atgatcgata tcagccagat
      481 cgacgggcag gtgcgcgaat cggccatccg caaagtgggc gatgtggtgc agtcccatcc
      541 cgaggaggcc atggcgatcc ttcgcacctg gcttcatcaa ccggtctaat ggattagctc
      601 atggcgcgcg caccgaaaac gcaggtcaag gaagatattc gcgccctctc cgggccggaa
      661 cgcgcggcga ttttcctgct ctcgctcggc gaagagcact ccacaaagct gtggcagatg
      721 atggacgagg acgagatcaa ggaagtctcg caactgatgt ccaatctcgg cacggtaagc
      781 tccagcctgg tcgagaaatt gctgatcgac ttcgtctccc agatgtcttc gacgggatcg
      841 ctgatggggt cctacgaatc caccgagcgg cttcttgccc gcatcctgcc cggcgaaaaa
      901 gtcggctcca tcatggagga aatccgcgga ccggccggcc gcactatgtg ggacaagctc
      961 ggcaatgtga acgagtcggt gctcgccaat tatctgaaga acgaatatcc ccagaccgtc
     1021 gcggtggtgc tgtccaagat caaatccgaa catgccgcgc gcgttctcgg cgccttgccc
     1081 gaggaattcg ccctcgaagt cgtgcagcgc atgctgcgca tggaatcggt gcagaaggat
     1141 attctggaca aggtcgagca gacgctgcgc gtcgaattca tgtccaacct cgcgcgcacc
     1201 gccaagcgcg atgcccatga gatgatggcg gagatcttca acaatttcga ccgccagacg
     1261 gagaaccgct tcctgacctc gctcgaagag cgcagccgcg actccgccga gcggatcaag
     1321 gcgctgatgt tcaccttcga ggatttgggc aagctcgatc cgggctccat ccagacgatg
     1381 ctgcgtcacg tggagaagga caaactcgcg ctcgccctga agggtgcgac ggattccttg
     1441 cgcgatgtgt tcttctccaa catgagcgag cgcgccggca agatcctgcg cgaagacatg
     1501 gaagccctcg ggcccgtgcg cctgaaggat gtcgacgaag cccagatgcg catggtgaac
     1561 gtcgccaagg atctcgccaa taagggcgag atcatgatcg ccagcaaaca aggcgaagat
     1621 gagttgatct attgatgggc accgcggtca aattcacctt cgacacggat ttcgacagcg
     1681 gcgcgccgga caccgcacgc gccgatgcgc gttcgcgcaa gaattacagc gccgaggaga
     1741 tcgaagagat caagcgcgaa gcccgcgatg aaggccatcg cgacggcaat gtccgcgcga
     1801 cccaggcggt cgccgcctcc atcggacagg tggcggcggc cgtgctgtcc gccatcgaag
     1861 cgatggacag cgagatcgaa gcgatccgca gtgaagccgc gggtctggcg gtcgccgctt
     1921 cgaagaaact cgcccgtgcg gcgctggcag cggcgccgga agccgaaatc gccgaggccc
     1981 ttcaggtcgc gcttcatcag gcgatcggcg aaccgcgggt cgtgatcaag acttcgccct
     2041 tgctcgcgca gaagattcag gagcgtgcga acgaaatcgc gaacgccgaa ggctacgaag
     2101 gccgcatgca gttcgtcggc gacggcggac tttccgatgc cgattgccgc atcgaatggc
     2161 gcggcggcgg catcgaacgc acgcaagccg cactggaaca cgcattgaac gatttggtcg
     2221 cgcggcgatt tgcgcgcgcg cccgctgagg agaacgaata atggcggata aggatgatgt
     2281 cggcctgccc gagcttggcg acgaccacgg cgcgcaagcc ggtcaaccgt tgctggaaaa
     2341 gcagagcggc ggcgatgccg atctgaaacg cgccgcgatc gatctggagg ccgtctacga
     2401 tgtgccggtc acggtgcagg cgatcctcgg caaatcggcg atggaagtca gccagcttct
     2461 gaagctcggc cgcggcaccg tggtgcagct cgaccgcaaa gtcggcgaag ccatcgacat
     2521 ctacgtcaac gaccggctgg tcgcacgcgg cgaagtggtg ctggtggaag accgtctcgg
     2581 cgtgaccatg acggaaatca tcaaatcggg ccaaatttag cgaatacgag gacatagaac
     2641 atgcggcttc tcatcatcgg cagcctcggc ggacagatcg gcgcggcaac ccggatcgca
     2701 ctcgacaagg gcgccaaagt tgcccacacc aacggcgtcg atcagggact ttctgagctt
     2761 cgccagggcc ggggcgccga ccttatcatg tgcgatgtcg cactggacat cggactgctc
     2821 gtccgctcgc tcgccgccga gcgcatctgc acaccggtca tcgcgtgcgg ggtgggaacc
     2881 gacgcgcgcc gtgccgtgga tgcgatccgg gccggcgcca aggaatatct gccgctgccg
     2941 cccgatcccg agcttatcgc ggccgtgctt gcggccgtcg ccgacgaatc ccatcaattg
     3001 atcttcgagg acgaggcgac agcctccgtc atcgccatgg ccgaccagat cgcgccatcg
     3061 gacgcctcca tcctggtcac cggcgaatcg ggcaccggca aggagattct cgcgcgctat
     3121 gttcatcgca agagcgcgcg agccgacaag ccgttcatct cagtcaattg cgcggccatt
     3181 cccgagaatc ttctggaatc ggaattgttc ggccacgaga agggcgcttt cacgggcgcc
     3241 atcgcccggc gcgtcggaaa attcgaggaa gccgacggcg gcacattgct gctggacgaa
     3301 atcagcgaaa tggacgcccg cttgcaggcc aagctgctgc gcgccattca ggaacgcgag
     3361 atcgaccgcg tcggcggcgg caaaccggtc agggtcaata ttcgtatcat cgccacatcc
     3421 aatcgcgacc ttagcgatgc ggtgaagcac ggcacgttcc gcgaggatct gctctaccgc
     3481 ctgaatgtcg tgaatctgag gctgccgtcc ttacgcgagc ggccccggga cattctcgcc
     3541 ctcgcccgcc atttcggccg caaatacgcc gaagcgaacg gcgtaccgtt ccgcccgctc
     3601 ggcggtgaaa ccgaacgcat gcttctcggc cacaattggc ggggcaatgt gcgtgagctt
     3661 gagaacacga tacatcgtgc cgtgcttctg tcgcacggca gcgagatcat gcccgaagca
     3721 atccgtctgc ccgatggcgg caagctgacc gagggcccgg tgcgcagcgg cagcgatgcg
     3781 gtcgcgattg ccaccgaagc cgccgccgcc gcaacgacgc gcgcgcttgt cgggcgtacc
     3841 gtgtcggacg tcgaacgcga tctgatcttg gacacgctcg accattgcct cggcaatcgc
     3901 acgcatgccg ccaacatcct gggcatctcc atccgcacgc tgcggaacaa actgcgcgaa
     3961 tatacccagt ccggcatccg cgtgacccag cctggcgaag ggcgcgcggc gtgacattgt
     4021 ctagggtcca atcgcaatga gcgttcaagc gcaaacattc cagagcggcc cgcgcggcgc
     4081 gttcgatgcc gtccgcgatt atctcaggaa cggcgaaatc gcactcgcct tcggcatcct
     4141 tgcgattctc gtcgtgctgt tgctgccgct gccgtcctgg ctgctcgacc tcgcgctctc
     4201 gttttcgatc accttttcga tcctgatcct gatgacatcc atcttcatca ggaagccatt
     4261 ggaattcagc tcgtttccca cggtgcttct catctccacc atgttgcggg tggcgctgaa
     4321 ccttgcctcc acgcgcctca tcctcgccca tggcaaggaa ggaaccgatg cggccggatt
     4381 cgtgatccag gccttcgggc gattgatcac gcaaggcaat ttcgtcatcg gcatcatcgt
     4441 cttcgcgatt ctcatcatcg tgaacttcgt cgtcatcacc aagggcgcgg gccgtatcgc
     4501 cgaagtcgcg gcgcgcttta ccttggacgc catgccgggc aagcagatgg ccgtggacgc
     4561 cgatctttcg gccgggctca tcaacgagac cgaagcgcgc gagcggcgta aatcgctgga
     4621 agccgaatcc aacttcttcg gcgccatgga cggcgcttcg aaattcgtgc gcggcgacgc
     4681 gatcgccgga ttgctggtcg tgggcatcaa cgtcatcggc ggcatcatca tcggtgtcgc
     4741 gcagcagggc atgagcttca ccgacgcttc gcagaccttc acgcttctga ccatcggcga
     4801 cgggctggtg acccaggttc ccgccctgat cgtatcgacc gccgccggca tgatggtgtc
     4861 caagggcggc accgagggct cctcggaaaa ggccttgctc ggtcagcttt cgttttatcc
     4921 ccaggcgctc gccatggcgt cattcgtcat ggccgtggtt tccatgctgc cgggcatgcc
     4981 gcatgtcgtc tttgcgctga tcgccttggg aacgggcggg ctcgcttacg cggcctggcg
     5041 caacaaggaa acgatccgcc acgacgccgc gcaaagcgaa gcggtgaaag cctctgaagc
     5101 gcctcccgtg gaagaaccga tttcgaccgc cctcgcgctc gatctcctcc gggtggagct
     5161 gggctacggc ctcttgccgc tcatcaacga cgccaagggc catcgcgtca ccgatcagat
     5221 caaggcgctg cgccgtcagc tcgcctccga gatgggcttc gtcatgcccg cggtgcgtat
     5281 cctggacaac atgcagcttt ccgcgaacga atatgtggtg cgcgtgaagg aagtggaggc
     5341 cggacggggc gaactctatc cgggccagct tctgctcatg gaccccagag gccaggcgat
     5401 cgagcttccg ggcacgcaca cgaccgagcc cgccttcggt ttgcctgcga catgggtgga
     5461 gcagggtctg cgcgaggagg cttcgttccg cggctacaca gtggtcgatc cgggaacggt
     5521 cttgaccacg catctgactg aagtgctgaa gacgcatatg tccgaactgc tctcctatgc
     5581 ggagaccaag aagctgctcg acgatctgcc gcccgaacag aagaagctgg tggaagagct
     5641 gatcccctcg caaatcccgg tcaccggggt gcagcgcgtg cttcagacgc tgctgtccga
     5701 acgcgtgtcc attcgcgatc tgcccgccat cctggaagga atcgccgagg cgctcggcca
     5761 taccaagaac gccatgctca tcaccgaaca tgtgcgcgcg cgcctgggcc gccagctttg
     5821 ccatcagcat ctgtcggcgc agggcgttct tccgattatc gcgatgtcgc cggcctggga
     5881 gcagaccttc gccgaatcgc tcgccgggac cggcgaggaa cgccagctcg ccatggcgcc
     5941 ctcgcaattg caggccttca tacaaaccgt gcgcgaacgc ttcgacgatg cgtcctcgcg
     6001 cggcgacctt ccggtgctgc tgacgagccc gctgatccga ccctatgtgc gttcgatcat
     6061 cgaacgcttc cggcctcaaa cgccggtgat ctcgcagaac gaaatccacc cttcggtcag
     6121 gcttcgcacc atgggccaga tctaatccat gcaactgcgg acgtttttcg ccaaggacat
     6181 gaaggaggcg ctgaccgcca tgcgtctcgc catgggcgaa gacgcgatca tcgtcgcgag
     6241 cgaaacgctg aaggacggaa cggtcctcct gcgcgccggc gccgaggaaa tccccattcc
     6301 caccggcgac g
//
LOCUS       DASYEG010000029         7231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_300410, whole genome shotgun sequence.
ACCESSION   DASYEG010000029 DASYEG010000000
VERSION     DASYEG010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 7231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7231
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300410"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..380
                     /gene="rpsQ"
                     /locus_tag="VGM72_01190"
     CDS             126..380
                     /gene="rpsQ"
                     /locus_tag="VGM72_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEY4263906.1"
                     /translation="MPKRVLQGVVVSDANAKTVVVKVERRFIHPVMGKTVRRSKKYHA
                     HDENKSHKVGDLVRIRECKPLSKLKHWEVIGRAGEAEGKA"
     gene            385..753
                     /gene="rplN"
                     /locus_tag="VGM72_01195"
     CDS             385..753
                     /gene="rplN"
                     /locus_tag="VGM72_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEY4263907.1"
                     /translation="MIQMSSELDVADNSGARRVMCIKVLGGSRRRYADVGDTIVVSVK
                     EAIPRGRVKKGEVLKAVVVRTAKGVRRPDGSLIRFDGNAAVLVNAQNEPIGTRIFGPV
                     TRELRAKNHMKIISLAPEVL"
     gene            753..1070
                     /gene="rplX"
                     /locus_tag="VGM72_01200"
     CDS             753..1070
                     /gene="rplX"
                     /locus_tag="VGM72_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEY4263908.1"
                     /translation="MSAKVRKGDRVVVTSGRDKGKSGEVLRVYPSDERVLVAGVNMVK
                     RHQRQTQRVQGGIVSKEAPLHLSKIAHVDPKTGAPTRIGFKVLQDGRRVRFAKKSGEV
                     LDV"
     gene            1225..1764
                     /gene="rplE"
                     /locus_tag="VGM72_01205"
     CDS             1225..1764
                     /gene="rplE"
                     /locus_tag="VGM72_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEY4263909.1"
                     /translation="MPRFKKHYLEVVRPELVQKFGYKNQLQAPRIEKIVLNIGAGEGA
                     ADQKKIQSAQNDLTAIAGQKAVITRAKKAIAAFKITAGRPVGVRVTLRKDRMYEFLDR
                     LITVALPRVRDFRGVSAKSFDGRGNYAMGLKEHVVFVEIDYDKVESVWGMDIVIQTSA
                     RTDDEAKALLAGFRLPFMN"
     gene            1788..2093
                     /gene="rpsN"
                     /locus_tag="VGM72_01210"
     CDS             1788..2093
                     /gene="rpsN"
                     /locus_tag="VGM72_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEY4263910.1"
                     /translation="MAKLSQINRNKKRVKMAAQYASKRTALKARADDMSVAPEERFAA
                     RLKLAKLPRNSSPTRIHNRCELSGRSKGFYRKVKLSRIALREFANFGQVPGMTKASW"
     gene            2103..2501
                     /gene="rpsH"
                     /locus_tag="VGM72_01215"
     CDS             2103..2501
                     /gene="rpsH"
                     /locus_tag="VGM72_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEY4263911.1"
                     /translation="MAINDPIGDLITRIRNGQLRGLSKISTPASNLRARLLDVLKLEG
                     FIRGYAEVEMKGKAKEFEIELKYHDGRPVIRELTRISTPGRRVYNSVDELKPHRSGLG
                     ISILSTPQGVMSDSDARAKNVGGEVLCRVF"
     gene            2518..3051
                     /gene="rplF"
                     /locus_tag="VGM72_01220"
     CDS             2518..3051
                     /gene="rplF"
                     /locus_tag="VGM72_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEY4263912.1"
                     /translation="MSRIGKKPVPLPQGVSAQVTDGTVSVKGPKGELKLKLVPEVEGA
                     VADGAIAISPREQTDRARAMWGMQRTLVNNLVRGVTEGFTEKLEISGVGYRAAVQGKN
                     LQLQLGFSHDVLHPIPAGIQIVCEKPTMITVTGIDKQLVGQVTAEIRSYRPPEPYKGK
                     GVKYAGERIRRKEGKKK"
     gene            3065..3427
                     /gene="rplR"
                     /locus_tag="VGM72_01225"
     CDS             3065..3427
                     /gene="rplR"
                     /locus_tag="VGM72_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEY4263913.1"
                     /translation="MANQKALFERRKRRVRFTIARVSGGRPRLSVHRSGRHIYAQLID
                     DAESKTIASASSSETLAEGTKAKTYNVAAASEVGKLIAQRAIEKGVKKVVFDRGGYLF
                     HGRVKALAEAARAGGLEF"
     gene            3478..4047
                     /gene="rpsE"
                     /locus_tag="VGM72_01230"
     CDS             3478..4047
                     /gene="rpsE"
                     /locus_tag="VGM72_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEY4263914.1"
                     /translation="MARESREREGRDRESELIDKLVHINRVAKVVKGGRRFGFAALVV
                     VGDQRGRVGFGHGKAREVPEAIRKATEAAKRSMIRVPLKDGRTLHHETHGHHGAGKVI
                     VRPADQGTGIIAGGPMRAVFESLGVGDVVAKSLGTSNPYNMVRATFDALKQQQSPRLI
                     AQRRGKSVSEIIQRREAQGKPEAEAAAGA"
     gene            4050..4268
                     /gene="rpmD"
                     /locus_tag="VGM72_01235"
     CDS             4050..4268
                     /gene="rpmD"
                     /locus_tag="VGM72_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041604457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEY4263915.1"
                     /translation="MAAKKPASDKSESGKKTVTVRQIRSEARQPAKMAATLRGLGLGR
                     IGRVKVLEDTPAVRGMIRQAAHLIAVSE"
     gene            4324..4764
                     /gene="rplO"
                     /locus_tag="VGM72_01240"
                     /pseudo
     CDS             4324..4764
                     /gene="rplO"
                     /locus_tag="VGM72_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
     gene            4989..6326
                     /gene="secY"
                     /locus_tag="VGM72_01245"
     CDS             4989..6326
                     /gene="secY"
                     /locus_tag="VGM72_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEY4263916.1"
                     /translation="MPSAAEQLASNFNLSALSKATELKQRILFTAVALIVARLGTYIP
                     MPGIDASELARQIQQQQGSLLDVFNLLAGGAIGRMAIFALGIMPYISSSIIIQLMTTV
                     SPTLEQLKKEGEAGRKQINQYTRYGTVMLAAVQAYAIAIGLEQWGTVVIAPGLAFRLS
                     TVITLTGGTIFLMWLGEQITARGVGNGISLIIMAGIVARIPTFLVQLFEQARTGAINP
                     ALIIGMFLLFGGLIAFVVFMERAQRRLLVQYPKRQQGNRVFGGESSHLPLKINVAGVI
                     PPIFASSLLLLPTTVANFNAANIPGWLQNIVVLLGHGKPLFILLYAAGIIFFCFFYTA
                     IVFNPEETADNLKKYGGFIPGIRPGKRTAEYIDYVLTRITVIGSLYLTAVCLIPEIIG
                     GAYNVQQFAFGGTSLLIVVSVTMDTVAQIQSHLIAQQYEGLIKKARLKGARPK"
     gene            6351..6944
                     /locus_tag="VGM72_01250"
     CDS             6351..6944
                     /locus_tag="VGM72_01250"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF011105.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEY4263917.1"
                     /translation="MNLILFGPPGAGKGTQAKILQAEWRLPQLSTGDMLRAAIAAGTE
                     LGRRCNDIMERGDLVPDEVVVGIIAERLDQPDCAHGAVFDGFPRTVRQAEALDGMLAG
                     RGSKIDAVIALKVDDEAMVGRMENRVKESPGSARADDNPETLKKRLTVYHQNTAPLLA
                     FYHHQGKLETVDGMEPIPQVAMAIGEILRRIAAQAVG"
     gene            7097..>7231
                     /gene="rpsM"
                     /locus_tag="VGM72_01255"
     CDS             7097..>7231
                     /gene="rpsM"
                     /locus_tag="VGM72_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEY4263918.1"
                     /translation="MARIAGVNIPTNKRVEIALRYIHGIGPAHAKDIIEKVGIAPDRR
                     V"
BASE COUNT         1507 a         2115 c         2362 g         1247 t
ORIGIN      
        1 ccggcaatga gccggtcaag gcggcaccgg ccgcgggcac aaagaagaaa gccgcggcat
       61 ccgctgccgg caaaacgaaa aagccggcca agcgcgccgg cgcgaagaaa taaggagaag
      121 cgcggatgcc gaaacgcgtg ctgcagggtg tggtggtgag cgatgcgaac gccaagaccg
      181 tcgtggtcaa ggtggagcgg cgcttcatcc acccggtgat gggcaagacc gtgcgccggt
      241 cgaagaaata tcacgcccat gacgagaaca agtcgcacaa ggtcggagac ctggtgcgta
      301 tccgcgagtg caagccgctt tccaagctga agcattggga agtcatcggc cgcgccggcg
      361 aagccgaggg gaaggcgtga ttccatgatt cagatgtcga gcgaactcga cgtggccgat
      421 aattccgggg cgcgtcgggt catgtgcatc aaggtgctgg gtggttcgcg ccgccgttat
      481 gccgatgtcg gcgacaccat cgtggtgtcg gtgaaggaag cgattccgcg cggccgcgtg
      541 aaaaagggtg aagtgctaaa ggcggtcgtc gtgcggacgg ccaagggcgt gcgccgcccc
      601 gacggttcgc tgatccgctt cgacggcaat gccgcggtgc tggtgaacgc ccagaacgaa
      661 cccatcggca cgcgcatctt cggtcccgtg acgcgtgagt tgcgcgccaa gaaccacatg
      721 aagatcattt cacttgcgcc ggaggtgctg tgatgtccgc caaagtcaga aagggtgacc
      781 gcgtggtcgt cacttcgggc cgcgacaagg gcaagtccgg cgaggttctg cgggtgtatc
      841 cgtccgacga gcgcgtgctc gtcgccggcg tcaacatggt caagcgtcat cagcgccaga
      901 cccagcgcgt ccaaggcggc atcgtgtcga aggaagcgcc gttgcatctg tccaagatcg
      961 cccatgtcga tccgaagacg ggggcgccca cgcggatcgg attcaaagtg ttgcaagacg
     1021 gccgccgcgt gcggttcgcc aagaaatccg gagaggttct cgatgtctga agcaggaacc
     1081 gccaaggaag gtaaagccgc cggtaagaaa cccgcgggcg gcgggggcgg caagcctccg
     1141 gacaagaagg gagaggccag ggccgcgaaa gcgtccaggt cgtcggggcc ggctgcgggc
     1201 gagacagagc acgatcccaa ttacgtgccg cgtttcaaga agcattacct cgaagtggtg
     1261 cgtccggagc tggtgcagaa attcggctac aagaatcagc ttcaggctcc ccgcatcgag
     1321 aagatcgtgc tcaatatcgg cgcgggcgaa ggtgcggccg atcagaagaa gatccagagc
     1381 gctcagaacg atctgaccgc catcgcaggc cagaaggcgg tcatcacgcg ggcgaagaaa
     1441 gccatcgcgg cgttcaagat caccgcgggc cgtccggttg gcgtgcgcgt cacgctgcgc
     1501 aaagaccgga tgtatgaatt tctcgaccgg ctgatcaccg tggcgctgcc gcgcgtgcgc
     1561 gacttccgcg gcgtctcggc aaagagcttc gatggccggg gcaattacgc gatgggactg
     1621 aaggaacacg tcgtattcgt cgagatcgat tacgacaagg tcgagagcgt gtggggcatg
     1681 gacatcgtca ttcagacatc ggcgcggacc gacgacgaag ccaaggcgct tctcgccgga
     1741 ttccgtttgc cgttcatgaa ttgaaagccg aaggagcgaa taggcctatg gccaagctga
     1801 gccagatcaa ccggaataag aagcgcgtaa agatggcggc gcaatatgcg tccaagcgca
     1861 cggccctgaa ggcgagagcg gacgatatgt cggtcgctcc ggaagaacgt ttcgccgcgc
     1921 ggctgaagct ggccaagctg ccgcgtaatt cctctccgac gcgcattcat aaccgctgcg
     1981 aattgtcggg ccgctccaag gggttttatc gcaaggtaaa actttcgcgc attgcgcttc
     2041 gggagtttgc caatttcggt caagtgcctg gcatgactaa ggcgagctgg taggagaacg
     2101 cgatggcaat caacgatcct attggcgatc tcattacgcg catccgcaac ggccagttgc
     2161 ggggtttgtc gaaaatctcg acgcctgcct ccaatctccg ggcgcggctg ctggatgtgt
     2221 tgaagcttga gggcttcatc cgcggctacg ccgaggtgga gatgaagggt aaggcgaagg
     2281 aattcgagat cgagctgaaa tatcacgacg gacggccggt gatccgcgaa ctgacccgca
     2341 tttcgacgcc gggccggcgt gtctacaatt cggtcgacga gttgaaaccg catcgcagcg
     2401 gattgggaat ctccatcctg tcgacgcccc agggcgtgat gtcggattcc gatgcgcgcg
     2461 ccaagaatgt gggcggcgaa gttctctgcc gcgtgttctg atgattggag acgattaatg
     2521 tcccgtatcg gcaagaagcc ggttccgctg ccccaagggg tctccgctca ggtgaccgac
     2581 ggcaccgttt cggtcaaggg ccccaagggc gagctcaagc tcaaactcgt gccggaagtg
     2641 gagggcgcgg tcgctgacgg cgccatcgcc atcagccctc gcgaacagac cgatcgcgcc
     2701 cgcgcgatgt ggggcatgca gcgcaccctg gtaaacaatc tggtgcgcgg cgtcacggaa
     2761 ggtttcaccg agaagctgga aatctccggc gtcggctatc gcgccgccgt gcagggaaag
     2821 aatctgcagc tgcaattagg cttttcccac gacgtgttgc accccattcc ggcgggtatc
     2881 cagatcgtct gcgaaaagcc gaccatgatc acggtcaccg gcatcgacaa gcagctcgtg
     2941 ggtcaggtga ctgcggaaat ccgcagttac cggccgccgg agccttataa gggcaagggc
     3001 gtgaaatatg ccggcgaacg tattcgccgc aaagaaggca agaagaaata aaagggcact
     3061 cgccatggcg aaccagaaag cattgtttga acgccgcaag cgccgggtgc gcttcactat
     3121 cgctcgggtc agcggcgggc gcccgcgtct gtcggttcat cgctccggca ggcatatcta
     3181 tgcccagctg atcgacgatg cagagagcaa gaccatcgcc tcggcgagtt cgagcgaaac
     3241 attggcggaa gggaccaagg ccaagaccta caacgtcgcg gcggcttccg aagtcggcaa
     3301 actcatcgcc cagcgcgcca tcgaaaaagg cgttaagaag gtcgtcttcg accggggcgg
     3361 ctatcttttt catgggcgcg tgaaagcgct cgcagaggcg gcccgtgcgg gcggccttga
     3421 attctaggaa gaccgagggc ccgcgcgacg cggcccgagg aaaggaaagg acatcccgtg
     3481 gcacgtgaat cccgcgagcg tgaaggccga gaccgcgaaa gcgagctcat cgataagctc
     3541 gtgcatatca accgcgtcgc caaagtggtg aagggcggca ggcgctttgg ctttgccgcg
     3601 ctcgtcgtgg tcggcgatca gcggggtcgt gtcggctttg gtcatggcaa ggcgcgcgag
     3661 gtgcccgaag ccatccgcaa ggcgacggaa gccgccaagc ggtccatgat ccgcgtaccg
     3721 ctcaaggacg gccgcacgct gcatcacgaa acccacggtc atcatggcgc gggcaaggtc
     3781 atcgtgcgcc cggccgatca ggggacggga atcatcgcgg gcggaccgat gcgcgcggtg
     3841 ttcgaatcgc tgggggttgg cgatgtcgta gccaagagcc tcggcacctc gaatccctac
     3901 aacatggtgc gcgccacctt cgatgcgctg aagcagcagc aatccccgcg cctgattgcg
     3961 cagcgccgcg gcaagagcgt ttcggaaatc attcagcgcc gcgaagccca gggcaagcct
     4021 gaagcggaag ccgcggcggg agcatgacca tggcagccaa gaaacccgcc tcggacaaaa
     4081 gcgaatccgg aaagaagacc gtcaccgtgc gccagatccg cagcgaggcg cgtcagcccg
     4141 cgaagatggc ggcgacattg cggggcttgg gcctgggccg gatcggacgc gtcaaggtgc
     4201 tcgaggatac gcccgcggtg cgtggtatga tccgccaggc ggcccatttg atcgcggtga
     4261 gcgaataatt tgttgcgtgt cgtggcgcat cggcggccat gacggaaaga gaacaggcag
     4321 gtcatgaaac tgaacgaact cagcgacaat gcaggcgcga gaagttcgcg cacgcgggtg
     4381 ggacgcgggt cgtcctccgg gctcggcaag acatgcgggc gcggcgtcaa gggcgccaag
     4441 gcgcgcgcgg gcgtgtccat ccatggtttc gaaggcggcc agatgccgct ttacatgcgt
     4501 ctgcccaagc gcggcttcca caatcatttc ggcaatgagt tcgcggtcat caatctcggc
     4561 cgcatccaag cggcgctcga ttcgggaaag cttaaggccg gcgatacgat ctccggcgag
     4621 gctctggcga aggccggaat cgtgagccag gccaagcacg gtgtgcgcct gctcggcaag
     4681 ggcgaactca aggcaaagct gatttttgag gttgccggag catcggcatc ggccatcgag
     4741 gccgtggaaa aggccggagg ctcggtcacg acctcgttca agaagacggt ccacctgaat
     4801 aagaaaggcg agcccgggaa gcgcgtccag cggcgcaacg cggcggccga gaagcggagg
     4861 agcgcggttc agccttaggc gcctttgctg gaaaagaagg cccgccgtcc catatgggaa
     4921 agaggtccga attttcctga aagcctatat actgccttca gaccgcgaat ccggtgccgg
     4981 agccgatcat gccctcagca gccgaacaat tagcctcgaa tttcaatctc tccgccctgt
     5041 cgaaggcgac ggagctgaag cagcgcatcc ttttcacggc ggttgcactc atcgtggcgc
     5101 ggctggggac ctatatcccg atgccgggca tcgatgcctc ggaattggcg cgccagatcc
     5161 agcagcagca gggcagtctg ctcgacgttt tcaatctgct cgccggcggc gccatcgggc
     5221 gcatggcgat cttcgcgctc ggcatcatgc cgtacatctc ctcctccatc atcatccagt
     5281 tgatgacgac ggtgtcgccc acgctggaac agttgaagaa ggaaggcgaa gccggccgca
     5341 agcagatcaa ccaatatacc cgctacggaa cggtgatgct ggccgcggtg caggcttacg
     5401 ccatcgccat cggattggag caatggggca ctgtggtcat cgcgccgggc cttgccttcc
     5461 gcctctccac ggtcatcacg ctgaccggcg gcacgatctt cctgatgtgg ctcggcgaac
     5521 agatcaccgc acgcggggtc ggcaacggca tctcgctcat catcatggcg ggcatcgtcg
     5581 cgcgcatccc gaccttcctg gtgcagctgt tcgaacaggc ccgcaccggc gcgatcaacc
     5641 cggcgctgat catcggcatg ttcctgctgt tcggcggtct catcgccttt gtcgtcttca
     5701 tggagcgcgc gcagcgaaga ttgctggtgc aatatcccaa gcgacagcaa ggcaaccggg
     5761 tctttggcgg cgaatcttct catctgccgc tgaagatcaa tgtcgcgggc gtcatcccgc
     5821 cgatctttgc ctcgtccctg ctgctgctgc ctaccacggt cgccaatttc aacgcggcca
     5881 acattccggg ctggttacag aatatcgtgg tgctcttggg ccatggcaag ccgctgttca
     5941 tcctgctcta tgcggcgggc atcatcttct tctgcttctt ctacacggcg atcgtcttca
     6001 atccggaaga gacggcggac aatctgaaga aatatggcgg cttcatcccg ggtatccgcc
     6061 cgggcaagcg cacggcggaa tatatcgact atgtgctgac ccgcatcacc gtcatcggct
     6121 cgctgtatct gaccgcggtc tgcctcattc cggagatcat cgggggtgcc tataatgtgc
     6181 agcaattcgc ctttggcggc acctcgctcc tgatcgtggt cagcgtcacc atggatacgg
     6241 tggcccagat tcaaagccat ctgattgctc agcaatatga aggcctgatc aagaaggcac
     6301 ggctcaaggg ggcgcggccg aaataaacgg ctcgcaacgg cgacacgctc atgaatctca
     6361 tcctgttcgg tcctccgggc gcgggcaaag gtacgcaggc gaaaattctg caggcggaat
     6421 ggcgcctgcc tcagctgtcg accggcgaca tgctgcgcgc ggcgattgcg gcgggaaccg
     6481 agcttgggcg gcgctgcaac gacatcatgg aacgcggcga ccttgtgccg gacgaggtgg
     6541 tggtggggat catcgccgag cgcctggatc agcccgattg cgcgcatggc gccgtgttcg
     6601 acgggttccc gcgcacggtt cggcaggccg aagcgctgga tggcatgctc gcagggcgcg
     6661 gcagcaaaat cgatgcggtg atcgcgctca aagtagatga cgaggcgatg gtcgggcgca
     6721 tggaaaatcg ggtgaaggag agccccggct cggcgcgggc cgacgacaat cccgagacat
     6781 tgaagaagcg tttgaccgtc taccatcaga acacggcacc cttgctggcc ttctatcacc
     6841 accaagggaa gctcgagacg gtggacggca tggaaccgat tccccaggtg gccatggcca
     6901 ttggcgaaat ccttcgccgg atcgcggcgc aggctgtggg ataaaggctt ggaaagccag
     6961 gattttgggc tggagtttga ggtattgact aaaggttatc gcaacctata atccgcgccc
     7021 tcgcggcggg attctttggt aattccggtg caaccatccg cgcgtctgcg ggtcatttga
     7081 ataaggagac atcaccgtgg cgcgcattgc cggcgtcaat atcccgacca acaagcgcgt
     7141 ggaaatcgcg cttcgctata ttcacggcat cgggcctgcc cacgccaagg atatcatcga
     7201 gaaggtcggc atcgcccccg atcgccgggt c
//
LOCUS       DASYEG010000030        17511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4938296, whole genome shotgun sequence.
ACCESSION   DASYEG010000030 DASYEG010000000
VERSION     DASYEG010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 17511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17511
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4938296"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VGM72_01260"
     CDS             <1..492
                     /locus_tag="VGM72_01260"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY4263919.1"
                     /translation="SDEGWGRGAHPVIFVSWNDAQTYIAWLRNKTGKPYRLLSEAEWE
                     YAARADTVSAYYWGPRAAGNWAKYGGSSGSASVGSYMPNSFGLYDTAGNVSEWAMDCL
                     NKTYDGAPTDGSAWMTGDCSLHALRGGAWNSPAANLRSANRDWDAAGFRVNRNGFRVA
                     RGL"
     gene            536..967
                     /locus_tag="VGM72_01265"
     CDS             536..967
                     /locus_tag="VGM72_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263920.1"
                     /translation="MTYTVDQLLATPQADLDKLFSASEAGPIPDGEAHGTAIIAPGTK
                     FSGETAKLINFFAWQGKVFDAQKGLLKNKVSSFGFHAIVARVYKGASWLDGKECIVLD
                     YSDTSIVAQWIRDEIRQIQPNMYLGVVYWNKARLIDFTLEF"
     gene            1071..5045
                     /locus_tag="VGM72_01270"
     CDS             1071..5045
                     /locus_tag="VGM72_01270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEY4263921.1"
                     /translation="MAAKGLQLGLGVERWIERRARGALHLLRRLDPFYRPFFNSTLRE
                     PIAAVVQALYNWRRPNLGLALAEEKTMPGEAEALASIIQDMGAYMRAMYKPGTFLRVG
                     NTKTHGVVRGEVIVRDDVPAHMRRGIFAEPRTYKSWVRFSGPGPDSPADIDDVGFGSI
                     AIKMMGVPGEKLLEDEKFTQDLICVSTPTFVTPDIVENALLQTQSNLRGTPFMYFWRP
                     GATHILDFLMQGLWNETKTSPLESQYWSCVPYLLGEGQAMIYSVRPKVWTRSRVPNLP
                     LRPPDNYLRDALLDTLAVREVEFDILVQVQTDPHRMPVENAGVRWPETLSPWVPVATL
                     RLFQQAHPWPAQLDLANRLSYNPWHSLKEHRPLGNQSRARFEMYQTLARLRQTENRTP
                     HIEPTGDEILETIFDDPARDDGAAWEDQGSVTLYAAVRPDSIDALKQELAAIQSRVQA
                     DGMPFKRSRRLHYARFVFVEETIDAGLGHCGPGLAYQGDFDGPRKRHLTELADFAADE
                     LNRIGRFLVDPIGPDAASRLQWLQDHIIPDATFYVNAVGRTVRQIRSEARLRARIEDF
                     LDQNKTPISDPKSLHWHILDFVRSEPSLKWALTPVRDLRWRIRRTIARIVLILIAIPL
                     VIVLSPLLLLWAIAIRWHEKIEHGEPPIPEPAHVAELANAEDGTLQNQFSAVGFRKAS
                     WLRLISPVIFLWLAKIVVRYFFDTNNLANLKTIHFAHWTFIDGKRRMLFTSNYDGSHE
                     NYMGDFIDIVGWGLNASFSHGVQYPRTRWLIVDGAWNERTFKRHNRNRQITTQVWYAA
                     YPNLSAVNIAENAKLRASLSARQNDGQGIDWLKLLRRDWGRPAAAPIVLEREDMQALL
                     TRGHSHHKAACFFLLTFADGADGIAAAKRWLSELIADREVTMNGKREDPCAHLAFTVD
                     GLRHFGCSESLISGFSDEFQFGMATEHRQRLLGDTGASSPQHWEWGRPGERLHAVLML
                     YTADQDELTRFRNRYDVSFKQHGIHATARETTWLADKKEHFGFHDGITTPPIEGIKPD
                     PASNLSIKPGEFIFGYQNEYHRYPTSPLIHRALDPQGHLKLDVEGSGMADFGRNGSYM
                     VFRQLRQDVFGFWSFMDRCAHALDGASDKRDWIAAKMVGRWRSGAPLMLTPDRDDPSL
                     ANSDDFMFHKADRLGLRCPVGSHIRRTNPRDSLDPQPGSAESLSLSKLHRLLRRGRTY
                     GDCLAGMDPEAILAAGDDGLDRGLHFICLNANISRQFEFVQSSWSNNPQFGALYDDVD
                     PLTGERGRYQTSNGAPGTANFTIPHDAGRTRIRGIPNFVTMRGGAYFFMPAMSALRYL
                     ATLP"
     gene            complement(5024..6895)
                     /locus_tag="VGM72_01275"
     CDS             complement(5024..6895)
                     /locus_tag="VGM72_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY4263922.1"
                     /translation="MAAGEMEYDYIVVGSGAGGGTVAARLAEAGMRVVLLEAGSDSGA
                     IDAARLPGDYEVPAFHTFASENPAMRWDFFVNHYTGTARQQRDSKCTPQGIYYPRAGT
                     LGGCTAHNAMIFMAPHDVDWDGIAELTGDKSWRAAKMRRYFHRIEDCRYHPFWRVLAW
                     LGFNPTGHGWAGWLQTQVALPRVVFRDDAMTHFVRASTWASVSRAPKPLRALWRLIKG
                     EADPNNRRVSRGRFEGVCYTPLATRGHARGGARERVLDAAARCPGRLRIETNALATAI
                     VFDEDNRAIGVDYLKGENLYRAHAMPADGPGRKQRVHARREVIVCGGTFNTPQLLMLS
                     GIGPAEDLRAHGICVRVDLPGVGRNLQDRYEIGVVYHMTQSWKSLNGARFEQDDPLYR
                     AWRDNRQGMYISNGAAISLVRRSAEHLSAPDLFCMALVARFEGYFPGYSRLVADHTDY
                     LTWAILKGHTQNRAGTVGLRSADPRDMPRIDFHYFDEGDDPGERDLKAVVEGIRFARC
                     IMASVNARGEIAREEKPGADIQSDDALAQYVRDNSWGHHASGTCAIGPREANGVLDGN
                     FSVHGTKGLRVADASVFPRIPGLFIVAAVYMIAEKAADAILAGAKSEHGAHQGSVAR"
     gene            complement(6915..9401)
                     /locus_tag="VGM72_01280"
     CDS             complement(6915..9401)
                     /locus_tag="VGM72_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263923.1"
                     /translation="MLPIVTFIRRAMLLSALLMLAACNRGETGPGHVLDEARLAGRDA
                     ASFPHAEEDYFRDMDRGLALSPDEIKGRNMWLVWSGGNDRFWDKMTGYTFGSFDLLKI
                     VTSHPSQTNSNSRDKRWAWLGLINEPCFKDAAGPDPNRFGLYLDVRDPSQDPGCAAAD
                     PFENETKYPGVKIGARGTTVPVGSYYGYASGIVGLRLFPNPDFDETAKKNWDPQKYYT
                     DPAYYNNPKLVRPYRVGMSCGFCHVGPNPVHPPANAAHPQFADLSSTVGAQYMWVDRL
                     FIYSANTSNFMYQLVRTYRPGTMDTSLVSTDSINNARTMNAIYDLGTRLAIGLRIAQE
                     KLAGGELNNKQFNDFPRTAALNNLFQKPDTVFTPHVLKDGADSVGGLGALNRVYLNIG
                     LFSEEWLLHFNPVVGGKPITPIAIADAEKNSSYWQATEAGTPDTALFFLKSTVPDHLK
                     EAPGGDKYLADSAAVLDRGKTVFAETCARCHSSKAPPVPPDRSIANCIGPNYLDCFKR
                     YWAYTQTADYKTQMNAIVHAPDFLDGNFLSTDARIPVTLLRTNACSPLATNGIRGNVW
                     DNFTSESYKNLPSVGTVTTYDPYTGDKVPYKMPAGGRGYTRPPSLIALWSSAPYLLNN
                     SVGTFNQDPSVDGRMSAFNESIEEMLWPEKRKRDTVLGDKLPGVIDRTTERSYVYIPV
                     GYLPDALRPLAGSFAQAGSIPIGPIPAGTPVGLLANTKLRPEDGDNPAEHAANLLKLT
                     AALVSNAPTIATANQIGDDALRQKLASVRAPFLALSKCPDFVVNRGHYFGTAAFNEQE
                     GLSADEKAFGTEPALSDDDKRALIAFLKTL"
     gene            complement(9502..10650)
                     /locus_tag="VGM72_01285"
     CDS             complement(9502..10650)
                     /locus_tag="VGM72_01285"
                     /inference="COORDINATES: protein motif:HMM:NF021508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HEY4263924.1"
                     /translation="MKNIAIFCDGTWQDLTQNVPTNVARLARSVAAQTKATDKTPACE
                     QFVYYDTGVGVGQGVLDGATKLIGGSIGKGLDDKILNAYIALCLNFSPCDRIFIFGFS
                     RGAYTARSLAGLLRRCWILRREHVGEADRAMDLYRNSTSEAPEVAKFKEDFCYPADAF
                     IGQRGADPVDTAKQLNAAPAFWGHLQYVGVWDTVGSLGIPTALPFSSLVDNKYRFHDT
                     TLSRFVLSARHAVSIDERRSTFVPTLWDNIDSLNINAFAGNLPYEKRPYQQIWFPGRH
                     SGVGGGEDDGGLSIVPMRWIAEGAAAAGLEFNDDVLDLPPTADCCAPFVAVQRTIGTL
                     VIEAAGEGDRGGPASTTEISDAARTRWTKLADYRPPPLSKFHDFLDAK"
     gene            10874..11185
                     /locus_tag="VGM72_01290"
     CDS             10874..11185
                     /locus_tag="VGM72_01290"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HEY4263925.1"
                     /translation="MKIDQNFDARSVTIPVGEAIELSLPENPTTGFRWALIGTGAPVC
                     ALKADDFVPSSQKPGAGGTHNFTFLAHQSGETTISLRNRRKWGDADTGRDFTLNVRVS
                     S"
     gene            complement(11188..12003)
                     /locus_tag="VGM72_01295"
     CDS             complement(11188..12003)
                     /locus_tag="VGM72_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEY4263926.1"
                     /translation="MAEEPLPVQRIKKFGWVPDLPDARDHLYAAPLFSLGALPVKADL
                     RSQCPKTIYDQGQLGSCTGNAIACAVQFDRIKQKMTSAGTLTPSRLFIYYNERVMENS
                     VAQDNGAQIRDGIKSIAKVGVCFEEGANSWPYDITKFAKAPPAACVKAALANRATQYS
                     RLVATAPQLKGCLASGYPFVFGFSVYEGFESDAVAKTGKVMMPGPKEKLLGGHAVIAV
                     GYDDAQQRFTVRNSWGPKWGMSGYFTIPYAYLTDTNLADDFWTVRMIQSPGTA"
     gene            12226..13197
                     /locus_tag="VGM72_01300"
     CDS             12226..13197
                     /locus_tag="VGM72_01300"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4263927.1"
                     /translation="MKIYLWAAMALVAGFGVAGASAQDGANTIDSHLRAAKAAAEFDY
                     TGTLARTCVLPQTGPGRDVAPGPAPARETWFTEPAKVFDNLYFVGTKIHSSWALTTSD
                     GIILLDTLYEYASGEAIVGGLRKLGLDPANVKYVIISHAHGDHVGGAKLMQDQFHSRI
                     VMGAPDWDSIEQSKNQYPNGKPKRDIVAADGQKITLGDTSVTLVLTPGHTPGTVSMIF
                     QVKDNGRPLTVAYSGGTAFNFVNDVPHFETYINSQRKMAAAAASAGATIIMSNHSEFD
                     NAVTKVKLVSARKAGEPHPFEVGRDAVARYFKVMEECAQVARLKLIK"
     gene            complement(13203..14300)
                     /locus_tag="VGM72_01305"
     CDS             complement(13203..14300)
                     /locus_tag="VGM72_01305"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY4263928.1"
                     /translation="MERIDCAVIGAGVVGLAIARALALSGREVVVLESQGAIGTGISS
                     RNSEVIHAGMHFATGSLKARFCVAGNAMLRAFAASHNVPFRMVGKLIVAVNADEERAL
                     AELYRLGNANGVAGLELISGVDAMRMEPELHCAQALYSPNSGIVDVHSLMLALRGDAE
                     AHGAVIALKTPVEDGRSRSDGVLVRIGGPDATEILAKTVVIAAGLDACRLGRAFALSS
                     VPTPYLCKGNYFALTGKPPFRRLIYPIPETASLGVHYTCDLSGQGRFGPDAEWIETEN
                     YDVDPNRAGQFYRAIGRYWPAIANRTLEPAYSGIRPKIHAQGTPRPDFRIAGPGEHGA
                     AGIIALYGIESPGLTSSLAIADHVRDLAASL"
     gene            complement(14476..15744)
                     /locus_tag="VGM72_01310"
     CDS             complement(14476..15744)
                     /locus_tag="VGM72_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263929.1"
                     /translation="MLPSASLLAAPGMNNFFAVLKRHKIYRAGASFALVSSLLLAHGC
                     TAGHEEPLCVLSPDAMPQSWRPSGEMQKRLNAAPWSAAETQDAEYAVKTGLRELNDLF
                     STRPTAVSTLGSDAVESAIDASYAASNMPALRNAARDLARRNLTPLMARYRERGPATV
                     TCKEFPSLLPLTVHAHALLSPEDANVSAMVALTNAAYRACGSLDAVLGHDYRQILVAP
                     NASTDDIWDLVMWSITFTDAQTIPGIELPAAARDLPPALWRYLQRYPFPRASAYPDGA
                     RNKTFYDTAYLATHIAYIPTGYGRYRIYIADAPGLYRFLRENFYAVLQMGELDLTAEF
                     VDLFREYGCTEQNDLQLRDGTRYLLKLFHSAADRWMAYREPDEPADTGDYNIVHKAWT
                     GMAGVRVRVPEPPAPHTYGGVVRGWLGDPR"
     gene            complement(15810..16850)
                     /locus_tag="VGM72_01315"
     CDS             complement(15810..16850)
                     /locus_tag="VGM72_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4263930.1"
                     /translation="MALRFSIRKWVRRLALTGIALAALSAQNGSSAPDRMPVRIEVPS
                     TNNLQFLTLWVALGAKYFQQEGLEPRIVAASTPRNTGDLLLRGEADVALLPPPMFLGM
                     MAEQKPIVLFASLLANEPINLIVRQDIGEARKIRANASLRERLRTIAGLKVGLASEVA
                     PRLRILFASAGMNADKDLQLVVVAGPDQLRAFADGRVDALFAHTPYLETALVQHHAIL
                     IADNSAGEVEALAKGQIHALATTRAQARANPALIAAVARAIARAQILIHSDSKTTVDA
                     IIASGAAAQANRQQLEAIVAIYGPAVPRTPQISLSGIARDATLYPAHPRAPDFRRTKV
                     ADYVAAEFAKPN"
     gene            17182..>17511
                     /locus_tag="VGM72_01320"
     CDS             17182..>17511
                     /locus_tag="VGM72_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263931.1"
                     /translation="MAVLTVSARAAESRVVVLTSYPDELTSAYEKAFEQAHPGIDVQI
                     VWQQGRDAMATLKKPDHGGIDVYWAPALFNFPALAQSGTFEKFQVDRSQVPGHIGAEA
                     ISDSNGYF"
BASE COUNT         3450 a         5260 c         5393 g         3408 t
ORIGIN      
        1 tccgatgaag ggtggggccg cggcgctcac cccgtcatct tcgtgagctg gaacgatgcg
       61 caaacctata tcgcatggct gcgcaacaag accggcaagc cctaccgcct gctgtcggaa
      121 gcggaatggg aatatgccgc ccgggccgac accgtgtcgg catattattg ggggccgaga
      181 gcggccggca attgggcgaa atatggcggc agcagcggaa gcgcttcggt gggcagctat
      241 atgcccaatt cgtttggcct ctacgacacg gccgggaatg tttcggaatg ggcgatggat
      301 tgcctgaaca agacctatga cggcgcgcca accgacggca gcgcctggat gacgggagat
      361 tgtagcctgc atgcgcttcg cggcggcgcg tggaacagcc ccgccgccaa tctgcgttcg
      421 gccaatcgcg actgggacgc ggcgggattt cgcgtcaacc ggaacggctt ccgtgtcgcg
      481 cgtggacttt gatgttatgg tcaatcgatt ttgaaccgag tggacggggg ctcagatgac
      541 ttatacggtc gatcaattgc tggcgacgcc gcaggcggat ctggacaaat tgttcagcgc
      601 cagcgaagcg ggtcccattc cggacgggga agcccatggc accgcgatca ttgcaccggg
      661 cacgaagttc agcggcgaga cggcgaagct gatcaatttt tttgcctggc agggcaaggt
      721 gttcgacgcc caaaagggat tgctgaagaa caaggtctcc tcattcggtt ttcacgccat
      781 cgtggcgcga gtctacaagg gcgcgagctg gctcgacggc aaggaatgca tcgtcctgga
      841 ttattccgac acctccatcg tcgcgcagtg gattcgcgac gagatccgcc agatccaacc
      901 gaatatgtat cttggcgtgg tctattggaa caaagcgcgg ctgatcgact tcacgctcga
      961 attttgaacg ggggcgtggc cgcggctcat cgatgatcgc ccggcaacag ccgttcatgg
     1021 ccgttgcgcc cgtatttttc agcgatctca aagagtgcag ggaggaagca gtggccgcta
     1081 agggtctgca gcttgggctg ggggtcgaga ggtggatcga acgccgcgcg cgcggcgcgc
     1141 tgcatctcct gcggcggctc gatccctttt atcggccgtt cttcaacagc acactgcgtg
     1201 aaccgatcgc tgcggtggtg caggcattgt acaattggcg ccgtccgaac ctgggcctcg
     1261 ctctggccga ggaaaagacc atgcctggcg aggccgaagc cctcgcttcg atcatccagg
     1321 acatgggcgc ctatatgcgc gcgatgtaca agccgggcac cttcctgcgc gtcggcaaca
     1381 ccaagacgca tggcgtggtg cgtggcgaag tcatcgtccg cgacgatgtc ccggcccata
     1441 tgcgccgcgg catcttcgcg gaaccgcgca catataaaag ctgggtgcgt ttttccggac
     1501 ccgggccgga cagtcccgcc gatatcgacg atgtcggatt cggcagcatc gcgatcaaga
     1561 tgatgggcgt ccccggcgag aaattgctcg aggacgaaaa attcacccaa gacctgatct
     1621 gcgtcagcac accgacgttc gtcacgccgg atatcgtcga gaacgcgcta ctgcagaccc
     1681 agtccaatct gcgcggcacg ccgttcatgt atttctggcg tccgggcgcg acccatatcc
     1741 tcgacttcct gatgcaagga ctgtggaacg agaccaagac cagcccgctc gaatcgcaat
     1801 attggagctg cgttccgtat cttctcggcg aaggccaggc catgatctat tccgtgcggc
     1861 cgaaggtatg gacgcgcagc cgcgtcccga acctgccgct ccgtccgccc gacaattatc
     1921 tgcgtgacgc gcttctcgac acgttggccg ttcgcgaggt ggaattcgac atcctggtgc
     1981 aggtccaaac cgatccccac cgcatgccag ttgagaacgc cggcgtgcgc tggcccgaga
     2041 cgctgtcgcc ctgggtgccg gttgcgacgc tgcggttgtt tcagcaggcg catccttggc
     2101 cggcgcagct cgatctggcg aatcggcttt cctacaatcc ctggcatagc ctcaaggaac
     2161 acaggccgct cggcaaccaa agccgcgcgc gcttcgagat gtatcagacg cttgcccggc
     2221 tcaggcagac ggaaaatcgt accccccaca tcgagcccac gggcgatgag attctcgaaa
     2281 ccattttcga cgatccggca cgcgacgacg gcgcggcgtg ggaagatcag ggcagcgtta
     2341 cgctctacgc ggcggtgcgg cccgattcca tcgacgcgct gaaacaggaa ttggcggcga
     2401 tccaatcgcg cgtgcaggcg gacggcatgc cgttcaaacg ttcccggcgc cttcattatg
     2461 cgcgatttgt gttcgttgag gagacgatcg acgccggact gggccattgc ggtcccggac
     2521 tcgcctatca gggcgatttc gacggaccgc ggaagaggca tttgaccgaa ctcgcggact
     2581 tcgcggccga tgagctgaac cgcatcggcc gtttcctggt ggaccccatc ggccccgatg
     2641 ccgcgagccg cctgcaatgg ttgcaggacc acatcatccc ggacgcgaca ttctatgtga
     2701 acgcggtcgg gcgtaccgtg cgccagattc gcagcgaagc aaggctgcgc gcgcgcatcg
     2761 aggacttcct cgaccaaaac aagactccga tcagcgaccc gaaatcgctc cactggcaca
     2821 tcctcgattt cgtgcgcagc gagccgtctc tgaaatgggc gctcacaccg gtgcgcgatc
     2881 tgcgctggcg cattcgccgg accatcgcgc ggatcgtgct tatcctgatc gctattccat
     2941 tggtcattgt cctttcgccg ctgctgctgc tgtgggccat cgccatccgc tggcatgaga
     3001 agatcgagca cggcgaacct ccgatccccg agcccgccca tgtcgcggag ctggcaaacg
     3061 ccgaagacgg taccctgcag aatcagttca gcgcggtggg tttccgcaaa gcctcctggc
     3121 ttcggctgat aagcccggtg atctttctgt ggctcgccaa gatcgtcgtg cgctatttct
     3181 tcgacacgaa taacctcgcc aatctgaaga cgatccattt cgcccattgg accttcatcg
     3241 atggcaagcg ccgtatgctc ttcacgagca attatgacgg cagccatgaa aactatatgg
     3301 gcgatttcat cgacatcgtc ggctggggcc tgaatgcgtc cttcagccac ggtgtccaat
     3361 atccgcgcac gcgctggctg atcgtcgatg gcgcctggaa cgaacgcacg ttcaagcggc
     3421 acaatcgcaa ccgccagatc acgactcagg tttggtatgc cgcctatccg aacctgtcgg
     3481 ccgtcaacat cgcggagaat gcgaaattgc gggcatcgct gtccgcacgc cagaacgatg
     3541 ggcaggggat cgattggttg aagctgttgc ggcgcgattg gggccggccg gccgccgcgc
     3601 cgatcgtact cgaacgcgaa gacatgcagg cgctcctgac gcgtggccac agccaccata
     3661 aggcggcgtg ctttttcctg ctcacattcg cggacggtgc ggacgggatc gccgccgcga
     3721 agcgttggct gagcgagctg atcgcggacc gcgaggtgac gatgaacggc aagcgcgagg
     3781 acccttgcgc gcatctagcc ttcaccgtcg atggtctccg gcatttcggc tgcagcgagt
     3841 ccttgatctc cggcttttca gacgagttcc agttcggcat ggcgacggag caccgtcagc
     3901 gcctgctcgg ggacaccggt gcgagctcgc cacagcattg ggaatgggga cgcccgggcg
     3961 agaggctcca tgcggtgctg atgctctata ccgccgatca ggatgagctg acgcggtttc
     4021 gaaatcgcta cgatgtaagt ttcaagcagc atggcatcca tgcgacggcg cgcgagacca
     4081 catggctcgc cgacaagaaa gagcatttcg gctttcatga cggaatcacc acgcccccta
     4141 ttgaaggcat caagcccgat ccggcgtcga atctcagcat caagccgggc gagttcatct
     4201 tcggctacca gaacgaatat caccgctatc cgaccagccc gctgatccac cgcgcgctcg
     4261 atccgcaggg gcatttgaag ctcgatgtcg agggatcggg catggccgat ttcggccgca
     4321 acggcagcta tatggtgttc cggcaattgc gtcaggacgt cttcggcttc tggtccttca
     4381 tggacaggtg cgcgcacgcg ctcgacggtg cttcggacaa acgcgactgg atcgccgcaa
     4441 aaatggtggg tcgttggcgc agtggcgccc ccctgatgct gacacccgat cgggatgatc
     4501 cgagtttggc gaattcggat gatttcatgt tccacaaagc cgatcgcctc ggattgcgct
     4561 gtccggtcgg atcgcacatc cggcgcacca acccacggga ttccctcgat ccccagcccg
     4621 gcagcgcgga gtcgctttcg ctgagcaagc tgcatcggct gctgcggcgg ggtcgcactt
     4681 acggcgattg cctggccggc atggaccccg aagccattct ggcggccggt gacgacggcc
     4741 tggaccgcgg gctgcatttc atttgcctca acgccaatat cagccgccaa ttcgagttcg
     4801 tgcaatcgag ctggtcgaac aatccgcagt ttggcgcgct ttacgacgat gtcgacccgt
     4861 tgaccgggga acgcggccga tatcagacca gcaatggcgc gcccggcacg gcgaatttca
     4921 ccattcctca cgatgccggg aggacgcgta ttcgcggtat ccccaatttc gtcacgatgc
     4981 gaggcggggc ttatttcttc atgcccgcga tgagcgcgct ccgctatctg gcgacgctgc
     5041 cctgatgcgc cccatgctct gatttcgcgc cggcgagaat cgcgtcggcg gctttttccg
     5101 cgatcatgta gaccgccgcc acgatgaaga gtcccgggat gcgggggaac accgacgcat
     5161 cggccacgcg caggcccttc gttccatgga cggagaaatt gccgtcgagc acgccgttcg
     5221 cttcacgcgg cccgatcgcg caggttcccg atgcgtggtg accccaggaa ttgtcgcgga
     5281 cgtattgggc aagcgcatcg tcgctctgga tatcggcgcc cggtttttct tcgcgcgcaa
     5341 tctcgccgcg ggcattgacg gacgccatga tgcagcgcgc gaagcgaatt ccctcgacga
     5401 cggctttcag gtcccgttct ccgggatcgt cgccctcgtc gaaataatga aaatcgatac
     5461 gcggcatgtc gcgcgggtcg gccgatcgca agccgacggt gccggcgcgg ttttgcgtgt
     5521 ggcccttcag gatggcccag gtcagataat cggtatgatc ggcgaccaaa cgggaataac
     5581 cggggaaata cccttcgaat cgcgcaacca gcgccatgca gaacagatcg ggagcggaga
     5641 ggtgttccgc cgaccgccgc acgagggaga ttgcggcgcc attcgagatg tacatgccct
     5701 gccggttatc gcgccaggcg cgataaagcg gatcgtcctg ttcgaagcgc gcaccgttga
     5761 gcgatttcca ggactgggtc atgtggtaga caacgccgat ttcgtaacga tcctgcagat
     5821 tccggccgac gcccggcaaa tcgacacgca cgcaaatgcc gtgagcgcgc aaatcctcgg
     5881 ccggcccgat gcccgacagc atcagcagtt gcggcgtatt gaatgtgccg ccgcaaacga
     5941 tcacctcgcg gcgcgcgtgg acacgttgtt tccggcctgg cccgtctgcc ggcatcgcat
     6001 gggcgcgata aagattttcg cctttcagat aatccacgcc gatggcgcga ttgtcctcgt
     6061 cgaacacgat cgcggttgcg agcgcgttgg tttcgatgcg caaccggccg gggcagcgcg
     6121 ccgccgcgtc cagcacgcgt tcgcgggcac cgccgcgcgc atgtccgcgc gtggcgagcg
     6181 gggtgtagca gacgccctcg aaacgtccac ggctgacgcg ccgattgttg ggatcggcct
     6241 cacccttgat cagccgccac agcgcacgca ggggtttggg ggcgcgcgat acgcttgccc
     6301 atgtgctcgc gcgcacaaaa tgggtcatcg catcgtcccg gaacaccacg cggggcagcg
     6361 cgacctgcgt ttgcagccag ccggcccagc catggccggt cgggttaaag ccaagccacg
     6421 ccaggacacg ccaaaatgga tgatagcggc agtcttcgat gcggtggaaa taacgccgca
     6481 tcttcgcggc acgccagctc ttgtctcccg tcaactccgc gatgccgtcc caatccacat
     6541 cgtgcggcgc catgaagatc atcgcattat gcgcggtaca gccgcccaac gtgccggcgc
     6601 gcgggtaata aatgccctgt ggcgtgcatt tggaatcgcg ctgctgacgg gccgtgccgg
     6661 tataatggtt gacgaagaag tcccatcgca tcgccgggtt ctccgacgcg aacgtatgaa
     6721 aggccggaac ctcgtaatcc cccggcagtc gcgcggcatc gattgcgcca ctgtcgctgc
     6781 ccgcttcgag cagcacgacg cgcattcccg cttcggcgag gcgcgcggcc actgtaccgc
     6841 cgccggcccc cgaaccgacg acgatgtagt cgtactccat ctcgccggca gccattatct
     6901 ttgcgagaat cgattcagag ggttttgagg aaggcgatca atgcgcgttt gtcgtcgtcg
     6961 ctgagtgcgg gctcggttcc gaaagctttc tcatcggcgg aaagaccctc ctgctcgttg
     7021 aacgcggcag tcccgaaata atgcccgcga ttgacgacga agtccggaca tttgctcagc
     7081 gccaagaagg gggcgcgcac gctggcaagt ttttgccgca gcgcgtcgtc gccgatctgg
     7141 ttggcggtcg cgattgtggg cgcgttgctg acgagcgcag cggtcaattt taggagattt
     7201 gccgcatgtt cggcgggatt gtcgccgtct tcggggcgca gcttggtgtt cgcgagcagc
     7261 cccaccggcg ttcccgccgg aatcggaccg atcgggatgc ttcccgcctg cgcgaatgaa
     7321 cccgcaagcg gccgcagggc atccggcaaa tagccgacgg gtatgtagac atagctccgc
     7381 tccgtcgtgc ggtcgatgac gcccggcagc ttgtcgccca gaaccgtgtc gcgcttgcgc
     7441 ttttcgggcc acaacatctc ttcgatggat tcgttgaatg cgctcatccg gccatcgacg
     7501 gaagggtcct gattgaatgt tccgacactg ttgttcaaca gatagggcgc cgacgaccac
     7561 agcgcgatga gtgaaggcgg acgcgtgtag ccgcggcccc cggcgggcat tttgtatggc
     7621 actttatcgc cggtataggg atcgtaagtc gtcaccgtgc cgaccgacgg gaggtttttg
     7681 taggactccg atgtgaaatt gtcccagacg ttgccgcgaa tgccattggt cgctagcggg
     7741 ctgcatgcgt tggtccgcaa cagcgtgacc gggatgcgtg cgtcggtgga cagaaaattg
     7801 ccatcgagaa aatcgggcgc atgcacgatg gcgttcattt gcgttttgta atcggccgtc
     7861 tgggtgtagg cccaatagcg cttgaagcaa tcgagataat tgggcccgat gcaattggcg
     7921 atcgaacggt cgggaggaac cggcggcgct ttgctggaat ggcatcgcgc gcaggtctcg
     7981 gcgaacacag ttttgccgcg gtcgagcacc gcggcgctgt cggcgagata tttatcgccc
     8041 ccgggcgctt ccttcagatg gtccggtacg gtcgatttga ggaagaacag tgcggtgtcc
     8101 ggcgttccgg cttcggtcgc ctgccaatag ctggaatttt tttccgcatc ggcaatcgcg
     8161 attggggtga tcggcttgcc gcccaccacc ggattgaaat gcagcagcca ttcctcactg
     8221 aacaatccga tgttgagata aacacggttg agcgcgccaa gcccgcctac ggagtcggcg
     8281 ccgtctttca gcacatgcgg cgtgaacacc gtgtccggct tctgaaacag attgttgagc
     8341 gcggcggtcc ggggaaaatc gttgaactgc ttgttgttca attcgccgcc cgcgagtttc
     8401 tcctgggcga tgcgcaaacc gatggccagc cgcgtgccga gatcgtaaat cgcattcatc
     8461 gtgcgggcat tgttgatgct gtcggtcgag accagcgagg tgtccatggt tccgggccgg
     8521 taggtgcgga cgagctggta catgaagttc gacgtattcg ccgaatagat gaaaaggcgg
     8581 tcgacccaca tatattgtgc gcccacggtc gaactgagat cggcgaattg cggatgggcg
     8641 gcattggccg ggggatggac cggattgggt ccgacatggc aaaagccgca gctcatcccg
     8701 acgcgatagg gacggacgag cttcggatta ttataatagg ccgggtcggt ataatatttt
     8761 tggggatccc agttcttctt cgccgtctcg tcgaaatccg gattgggaaa cagccgcaag
     8821 cccacaatgc cggacgcgta gccgtaatag gagcccaccg gaacggtcgt gccgcgcgcg
     8881 ccgatcttca cgcccggata tttggtctcg ttctcgaaag ggtccgccgc cgcgcagccg
     8941 ggatcctgcg aaggatcgcg aacatcgaga tagagaccga agcggttggg atcgggtccc
     9001 gccgcatcct tgaaacaggg ctcgttgatc agcccgagcc aggcccatct cttgtcgcgg
     9061 ctgttggaat tggtctggct cggatgggat gtgacgatct tcaaaagatc gaaagagccg
     9121 aaagtatagc ccgtcatctt gtcccaaaaa cggtcattgc cgccggacca caccagccac
     9181 atgttgcggc ccttgatctc gtcaggcgag agggcgaggc cgcgatccat gtcgcggaaa
     9241 taatcctctt cggcatgcgg gaaagacgcc gcatcgcggc ccgccaagcg cgcttcgtcc
     9301 agcacatggc ccggtcccgt ttcgcccctg ttgcaggcag cgagcatcaa caatgccgaa
     9361 agaagcattg cccggcggat gaacgtcacg attggcagca tcgttttccc cagcgcacga
     9421 atcccagcgg ttcgcttgcc gcgataggcc ggacggaccg ttaactatga gagcagaaca
     9481 taatcccgct cgccaggaaa atcacttggc atccaggaaa tcgtggaatt tgctcagcgg
     9541 cgggggccgg taatcggcga gcttggtcca gcgtgtccgc gcggcgtcgg aaatctccgt
     9601 tgtgctggcc ggtccaccgc ggtcgccttc gccggcggct tcgatgacga gggtcccgat
     9661 cgtacgctgt acggcgacaa agggcgcgca gcaatcggcc gtgggcggaa ggtctaagac
     9721 gtcatcgttg aattccagac cggccgcggc cgcgccttcc gcgatccaac gcatcggcac
     9781 aatcgacagg ccgccgtcgt cttcgccgcc gccgacgccg gaatgccggc ccggaaacca
     9841 gatctgttgg taaggacgct tttcgtaggg aagattgccg gcaaaggcat tgatgttgag
     9901 agaatcgata ttgtcccaca gcgtcggtac gaaggtcgaa cgccgttcat cgatcgagac
     9961 ggcgtggcga gcgctcaaga cgaagcgcga cagggtcgta tcgtggaaac gatatttgtt
    10021 gtccaccagc gatgaaaatg gcagcgccgt tggaattccg agcgagccca ccgtgtccca
    10081 cacgccgaca tattgaagat gtccccagaa cgcgggcgcg gcattgagtt gcttggcggt
    10141 atcgaccgga tccgccccgc gctgtccaat aaaggcatcg gcggggtaac aaaaatcctc
    10201 tttgaatttt gccacctcgg gggcttccga tgtgctgttg cgatagaggt ccatcgcgcg
    10261 atccgcctcg ccgacatgtt cgcggcggag aatccagcaa cggcgcagca ggcccgccag
    10321 actgcgcgcg gtataggcgc cacgcgaaaa cccgaaaatg aaaatccggt cgcaaggcga
    10381 gaaattcagg cacaaggcaa tataggcatt caggattttg tcatcgagcc ctttgccgat
    10441 gctgccaccg atgagcttgg ttgcgccgtc caagacacct tgtccgacac cgacgcccgt
    10501 gtcgtaatag acgaattgct cgcaagcggg cgttttgtcg gtggccttgg tctgcgccgc
    10561 gacgctgcgg gcaaggcgag cgacattggt ggggacgttt tgcgtgagat cctgccaggt
    10621 gccatcgcag aaaatcgcta tgttcttcat cgcgggaacc tgcctgtcgt ctgagtggac
    10681 gtcgcgaaat gtgcccgaac tcgcccgtat gataaagcag attctggagc gtggctgtga
    10741 tgccggagtg cgccaatcgg catatgcgca tgcgatatgc gaaacgcgtt gcggcacggc
    10801 gcgttatctt gcctgccagc cggttgcgcc tgtccctctc atggggcgct cacacgcgag
    10861 aaacgatgcc aatatgaaaa tcgatcaaaa cttcgacgcg cggtccgtca ccatacccgt
    10921 aggggaagcg atcgaactta gccttccgga gaatccgacc acggggttcc gctgggccct
    10981 gattggcacg ggcgctccgg tctgtgcgtt gaaagcagac gatttcgtcc cctcgagcca
    11041 aaaaccgggg gcgggcggaa cgcacaattt cacgtttctg gcacaccaat ccggtgagac
    11101 gacgatctcg cttcgcaatc ggcgcaaatg gggagacgcg gacaccgggc gtgatttcac
    11161 actgaatgtc cgcgtctcgt cctgaattta cgccgtccca ggggattgaa tcatccggac
    11221 cgtccaaaaa tcatccgcga gatttgtgtc ggtgagatag gcatagggta tggtgaaata
    11281 accgctcatg ccccatttgg ggccccagga attgcgcacg gtgaaacgct gctgcgcatc
    11341 gtcgtaaccc acggcgatga ccgcgtgccc gccaagcagt ttttccttgg gaccgggcat
    11401 catgactttg ccggtttttg caaccgcgtc gctttcgaat ccctcataca cgctgaaacc
    11461 gaagacgaag ggatatcccg aggccaagca gcccttgagt tgaggcgcag tcgccacaag
    11521 ccgtgaatat tgtgtggcgc gattggctag cgcggcttta acgcaagccg cgggcggcgc
    11581 tttcgcgaac ttcgtgatgt cgtagggcca tgaattggcg ccctcttcaa aacagacgcc
    11641 gaccttggcg atggacttta ttccgtcgcg tatctgcgcg ccattgtctt gcgcgaccga
    11701 attttccatt acccgctcat tgtaatagat gaacagccgc gaaggggtca gcgtgccggc
    11761 gctggtcatt ttctgcttga tccggtcgaa ctgaacggcg cacgcaatcg cattgccggt
    11821 acagcttccg agctgaccct ggtcgtaaat cgttttaggg cattggctgc gcaagtcggc
    11881 tttcaccggt aaggccccca gcgaaaacag cggcgccgca tacagatggt cccgtgcgtc
    11941 cggaagatcg ggcacccagc cgaatttctt aatacgttgc acaggcaagg gttcttcagc
    12001 cattcgtctc tcctacattt acgtgggacg cacgcctgat caggcgatga agcatcctag
    12061 ccatagatgc cgggattttg gaatagatcg tgtcgagcgc cggatttgat tgggcgatga
    12121 tatgtttgta tcttgcagcg cgtcgtcacc ggatattttc tccgcactcg gacgccgtct
    12181 ttttgtgcag cgtcgtttct tcgaattgca aatcgggagg gtgccatgaa gatttatctc
    12241 tgggccgcga tggcacttgt ggccggattt ggcgtcgcgg gcgcgtccgc acaggatggc
    12301 gcgaacacga tcgactccca cctgagggcc gcaaaggccg cagccgaatt cgactatacc
    12361 gggacgctgg cccgcacgtg cgttctgccg cagacgggtc ccggccggga cgtggcgccg
    12421 gggcccgcgc cggcgcgcga gacctggttc accgaaccgg ccaaggtatt cgacaatctt
    12481 tatttcgtgg ggacgaagat tcattcgtcc tgggcgctga cgacgagcga cggcatcatc
    12541 ctgctcgaca cgctgtacga atacgcatcc ggcgaagcca ttgtaggcgg attaagaaag
    12601 ctcggcctcg atccggcgaa cgtcaaatac gtcatcatct cgcatgcgca tggggatcat
    12661 gtcggcggcg ccaagctgat gcaggaccag ttccattcgc gcatcgtcat gggagcgccg
    12721 gactgggatt ccatcgagca atccaaaaac cagtatccga acggaaaacc caaacgcgat
    12781 atcgtcgccg ccgatggcca gaagatcacc ctcggcgata ccagcgtaac actggtgcta
    12841 acgcccggtc atacgcccgg caccgtttcg atgatcttcc aggtgaagga taatggcagg
    12901 ccgctgacgg tcgcgtattc cgggggcacg gcattcaatt tcgtcaatga cgtgccgcat
    12961 ttcgagacct atatcaattc ccagcgcaaa atggccgccg ccgccgcgag tgccggcgcg
    13021 acgatcatca tgtcgaacca ttcggaattc gataatgcgg tgacgaaggt caagctcgtt
    13081 tcggcacgca aggccggcga gccgcatccc ttcgaagtcg gccgcgacgc ggtggcgcgt
    13141 tacttcaagg tcatggagga atgcgcccag gtggcccggc tgaagctcat caaataggag
    13201 tctcagagac tcgcggccag gtccctgaca tgatcggcaa ttgcgagcga tgatgtcagg
    13261 cccggcgatt cgataccgta gagcgcgatg attcccgccg cgccgtgctc tcccggcccg
    13321 gcgattctga aatccggccg cggcgtgcct tgcgcgtgaa tcttggggcg tattccggaa
    13381 taggcgggct ccaatgtccg gttcgcgatg gcgggccaat aacggccgat ggcacgatag
    13441 aattgtccgg cgcgattggg gtcgacatcg taattctccg tttcgatcca ttcggcgtcc
    13501 ggcccgaagc gtccctgccc ggaaaggtcg caggtgtagt gaacgcccag gctggcggtc
    13561 tccggaatcg gatagatcag gcgccgaaac ggaggctttc ccgtcagcgc gaaataattg
    13621 cctttgcaca aatagggcgt gggcaccgag gacagggcga acgcgcgtcc gagacggcac
    13681 gcgtcgagac cggcggcgat caccacggtc ttggcgagga tttccgtcgc atcgggacct
    13741 ccgatcctca ccaatacgcc atcgctgcgg ctgcgcccgt cttcgaccgg cgttttcagc
    13801 gcgatcacgg cgccatgggc ttcggcatcg ccccgaagcg caagcatgag agagtgcaca
    13861 tcgacgatgc cgctattcgg ggaataaagc gcttgcgcgc aatgcagttc cggctccatg
    13921 cgcatggcgt ccacaccgga gatcagctcc agtcccgcaa ccccgttggc gttgcccaac
    13981 cggtacagct cggcaagcgc gcgttcttca tccgcattga ccgcgacgat cagctttccc
    14041 accatccgaa aggggacatt atgggacgcc gcgaatgccc ggagcatcgc gtttccggcg
    14101 acgcagaaac gggccttgag gctgccggtc gcgaaatgca tcccggcgtg gatcacctcg
    14161 ctgttgcgtg atgaaattcc ggtgccgatc gcgccctgcg attccaggac cacgacctcg
    14221 cgccccgaca gagcgagcgc gcgggcgatc gccaagccga caacgcccgc gccgatcacc
    14281 gcacaatcta tgcgctccat gcagccgtct ccggtttgaa aaaatgagtg tccgtcgacc
    14341 cacaaaaaac ggcgcgtgct attccagcga tcacgccatc aagcacgcgg ccgccgattg
    14401 ccgccggtct tatagcagaa gcggtgtcat cgcgaatttc cgctttatgt tccccatctc
    14461 ccccggcgag gcgttttaac gcggatcgcc aagccatccg cgaacgacgc cgccataggt
    14521 gtgcggggcg ggcggctctg gaacccggac acggactccc gccattccgg tccacgcttt
    14581 gtggacgatg ttataatcgc ctgtatcggc cggttcgtcc ggctcgcgat aggccatcca
    14641 acggtctgcc gccgaatgaa aaagcttcag cagatatcgc gtgccatcgc gcaattgcag
    14701 atcgttttgc tccgtgcagc catattcgcg gaacaggtcc acaaactccg ccgtcagatc
    14761 gagctcgccc atctgcaata cggcataaaa attctcgcgc agaaaacggt acaagccggg
    14821 cgcatccgca atgtaaattc gatagcggcc atagccggtt ggaatgtagg cgatgtgggt
    14881 tgcgagatag gccgtatcgt agaacgtctt gttccttgcc ccatcgggat aggcgctcgc
    14941 tcgcgggaac gggtagcgct gaagatagcg ccaaagcgcg gggggaagat cgcgggcggc
    15001 agcgggcaat tcgatgcccg gaatcgtctg ggcatcggta aaggtgatgc tccacatcac
    15061 cagatcccaa atatcgtccg tggaggcgtt tggggcgact aggatttggc gatagtcgtg
    15121 acccagaacc gcatcgagcg agccgcacgc gcgatatgcg gcattcgtga gcgccaccat
    15181 cgccgacaca ttcgcatcct ccggcgataa gagggcgtgc gcgtggacgg tcagtggcag
    15241 caaggacgga aattccttgc aggtgaccgt ggcggggccg cgctcgcgat agcgcgccat
    15301 caacggcgtc aggtttcggc gcgccaggtc gcgcgccgca ttccggaggg ctggcatatt
    15361 cgatgccgca taggaagcat ctatggcgga ttccaccgcg tcgcttccga gcgttgagac
    15421 cgctgtgggc cgggtggaaa aaagatcgtt cagttcacgc aaacccgtct tcacggcgta
    15481 ttcggcatcc tgcgtttcgg cggctgacca gggtgcggcg ttcaaacgct tctgcatctc
    15541 gccggatggc cgccagctct gcggcattgc atcgggagaa agcacacaca gcggctcttc
    15601 atgcccggca gtgcagccat gagccaacag cagcgacgac acgagcgcga aagacgcgcc
    15661 cgcgcgataa attttgtggc gcttcaatac ggcgaagaaa ttgttcatgc ccggtgccgc
    15721 cagcagggat gcggacggca gcattgctga agcccgcgag acaaatcaat ccgccaccgc
    15781 cggttatgga ctctggattt tatccgctgt cagttcggct tggcgaattc cgccgcgacg
    15841 taatcggcca ccttcgtccg cctgaaatcc ggtgcgcgcg gatgggcggg ataaagcgtg
    15901 gcatcgcgcg cgatgccgga gagcgagatt tgcggtgttc tcggcacagc cgggccgtag
    15961 attgcgacaa tcgcctccaa ttgctgccgg tttgcttgtg ccgcggcgcc ggaggcgata
    16021 atggcatcga ccgtcgtttt tgaatcggaa tggatcagaa tttgcgcgcg cgcgatggca
    16081 cgcgcaactg cggcaatcag cgccgggttg gcacgtgctt gtgcgcgcgt ggtcgctaaa
    16141 gcgtggatct gtcctttggc gagcgcctcg acctcgcccg ccgaattgtc cgcgatcaga
    16201 atggcgtgat gttgtaccag cgcggtttcg agatagggcg tatgggcgaa cagggcatcc
    16261 acacggccat cggcaaaggc ccggagctga tcggggccgg caacaacgac aagctgcaaa
    16321 tccttgtccg cgttcatgcc ggccgaagca aagaggatgc gcagccgtgg cgccacctcg
    16381 cttgccagac cgaccttgag tccggcgatt gtccgcaaac gctcgcgcaa cgatgcattg
    16441 gcgcggattt tccgggcttc tccgatatcc tgccggacaa tcaggttgat gggttcattg
    16501 gcgaggagac tcgcgaatag cacaatcggc ttttgttccg ccatcatgcc cagaaacatg
    16561 ggcggcggca gaagcgccac atccgcctcg cctcttaaca gcagatcccc cgtatttcga
    16621 ggcgtcgaag cggcaacaat gcgcggctcc aatccttcct gctgaaaata cttcgcgccc
    16681 agtgcgaccc agagcgtgag gaattgcagg ttgttcgtgc tggggacttc gatacggacc
    16741 ggcatccggt ctggcgcgga cgatccgttc tgcgcggaaa gcgccgccaa agcgattccc
    16801 gtcaacgcca atcgtcttac ccatttacgg atcgaaaaac gcaacgccac gtgaagcctc
    16861 tcattgccga tgcacgacgg taaacgatac ccggaaatat gctcgaaaaa acaacgcgca
    16921 gtcctatgag gttgaatccg tatggctgtc ctcacttttg tcttcggccc aagcaagctc
    16981 cgtggacgca caaccgggcc atggtcttag gccttgaata tcggcgggag accgtgatat
    17041 tcaaacgaat ataacaaata atttagagag attgtaatgc caaggggccg gttttgttgc
    17101 gagtgggacg aagtcgcctt cagggcgcct cgcgttggcc gggcatccgc aatcttgcgc
    17161 tatggcgcgg tatttctgtt cttggccgtt ctcacggtct ccgcgcgtgc ggcagagtct
    17221 cgggtcgtcg tcctcacctc ctatcccgac gaactgacga gcgcttacga aaaggcattc
    17281 gagcaggccc atcccggaat cgatgtgcag atcgtttggc agcaaggccg cgacgcgatg
    17341 gccacgttga agaagccgga tcatggcggc atcgatgttt attgggcgcc tgcgcttttc
    17401 aatttcccgg cattggcgca atccgggaca tttgaaaaat tccaggtcga tcgcagtcag
    17461 gtgccgggcc atatcggcgc ggaagccatt tcggattcga acggatattt c
//
LOCUS       DASYEG010000031         3847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_5083973, whole genome shotgun sequence.
ACCESSION   DASYEG010000031 DASYEG010000000
VERSION     DASYEG010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5083973"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VGM72_01325"
     CDS             complement(<1..566)
                     /locus_tag="VGM72_01325"
                     /inference="COORDINATES: protein motif:HMM:NF036854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263932.1"
                     /translation="MDFDKRVTPARLDLAAAHLEGKVTAAKFVEGRTGQVTRGVTGLQ
                     AAPTETAGLRTQLLFGEEFTVYEEKHGWVWGQAALDGYVGYARAGSVGTSCPPTHRVI
                     VLSTPLLNAPDVKKGARDMLPLNAKVAVAEEDGRFVRLSNAGYVFAGHLAPLGAQVPD
                     WVDMAERFLGVPYLWGGKTAAGLDCSGLV"
     gene            complement(569..1972)
                     /locus_tag="VGM72_01330"
     CDS             complement(569..1972)
                     /locus_tag="VGM72_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEY4263933.1"
                     /translation="MLSIFVNQTDAGAVTPVMLHAVLPKDLEKFLGAREPAQARWLKA
                     TGFSAKEGELVLVPAETGALGEAVLGLGTGRDPHALALFSERLPQGLYTLGDVPDAFS
                     GARAAYAWAIGTYAFDRYRKHKKSSRREPPRLVLPAGVDGETISRVAQGVFLGRDLIN
                     TPSNDMGPDELEQAARSLGESFGARLSVTAGDALLAANYPMIHAVGRASVRTPRLIDL
                     VWGDSSHPKVTLVGKGVCFDTGGLDLKNASGMATMKKDMGGAACVLGLARTIMDAKLP
                     VRLRVMVPAVENSVSGSAYRPGDVLPSRKGLFVEIGNTDAEGRLVLADALAEADTERP
                     DLMFCLATLTGAARAATGMELPPFFTDDEMLAADLMRHGAEENDPVWRLPLWRSYEAA
                     IDSQIADINNAPDYPLAGAITAALFLNRFITKTQSWAHFDIPAWVDRPKPGRRKGAEA
                     NAIRAMFAVIRARYEDR"
     gene            2053..2721
                     /locus_tag="VGM72_01335"
     CDS             2053..2721
                     /locus_tag="VGM72_01335"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY4263934.1"
                     /translation="MTARYTLFIGSKNTSSWSLRPWLAMKMAKLPFEDVLIVLRQPET
                     KEQIKPHSPSGKVPALRIADGATVETVWDSLAICETLAERHPDARMWPQDAAARAHAR
                     SIAAEMHSGFADVRNVLPMDIATRHPTPRLDDSTQTQIARIVAVWEETLSHYGKDGGF
                     LFGPFTIADAFYAPVATRFETYGIALPPAAQAYAQRVLSLGPMREWEAAARAEKTASP
                     SERP"
     gene            complement(2735..>3847)
                     /locus_tag="VGM72_01340"
     CDS             complement(2735..>3847)
                     /locus_tag="VGM72_01340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEY4263935.1"
                     /translation="NGSVLYSVMQAKRRVSWSLATGAIFALGVSLTLAIAATAQITDN
                     PQRIAFQISTGSTTGTYFPIGSMLAQLLSHPPGVGRCEAANICGPSGLIVSTRASQGS
                     IANVLAVNSGMVTSGLAQTDVIALAMAGQGPFRKAGPTKDVRVIANLYGEDLHLIAAK
                     GAKIKSVADLRGKRVSLSSEGSGTIVTARAVLAAYRLTEKSIIANYDSAEKAVDLLQA
                     GKLDALFFVGGTPVNLLQPLLDEDVAVLVPIDGDGLKRLLAKESFLSPHVIAKGTYAN
                     TPDLETVSVDALWITDASEPDALIYGMLKALYNPANRATLTAGKLGTHFLDETYGAKA
                     GMAPLHPGAARFFTEAGLLKPAQKAALPARSGSGRT"
BASE COUNT          729 a         1243 c         1198 g          677 t
ORIGIN      
        1 accagacctg agcaatccag gccggcggcc gtcttgccgc cccagagata gggcaccccc
       61 agaaatcgct cggccatgtc tacccaatcg ggaacctgcg cacccagcgg cgccagatgg
      121 cccgcgaaga cgtagcctgc attgctcaga cgcacaaaac gcccatcctc ttcggcgact
      181 gcgaccttgg cgttcagggg cagcatgtcg cgcgcgccct tcttcacgtc cggagcattc
      241 agcagaggag tgctcagcac gatcactcga tgcgtaggtg ggcaggatgt accgacgcta
      301 ccggctcgcg cataaccgac atagccgtcg agcgcggcct ggccccagac ccagccatgc
      361 ttttcttcgt agacggtgaa ttcctcgccg aacagaagct gcgtgcgcag cccggcggtc
      421 tcggtgggcg ccgcttgcag gcccgtgacg ccgcgtgtca cctgccccgt gcgtccttcg
      481 acgaacttcg ccgccgtgac cttgccttcg aggtgcgcgg ccgcgagatc gaggcgcgcc
      541 ggtgtcaccc gcttgtcgaa atccacgtct atcggtcctc gtagcgcgcc cggatgacgg
      601 cgaacatggc gcgaatggcg ttggcttccg cgcccttgcg ccgtcccggt ttcggccggt
      661 ctacccaggc cggaatatcg aaatgcgccc agctttgggt tttcgtgatg aagcggttga
      721 gaaacagagc cgccgtgatc gcgccagcga gcggataatc cggtgcatta ttgatgtcgg
      781 cgatctggct gtcgatcgcc gcctcgtagg atcgccataa gggaaggcgc caaaccggat
      841 cgttttcctc ggcgccgtga cgcatcagat cggcggcgag catttcatcg tcggtgaaaa
      901 aaggcggcag ttccatgccg gtcgcagccc gcgccgcgcc ggtgagggtc gccaggcaga
      961 acatcaggtc cggcctctcg gtatcggctt cggcgagcgc atccgccagc accaagcgcc
     1021 cttccgcgtc ggtattgccg atctcgacga acagcccctt gcggctgggg agcacgtcgc
     1081 cggggcgata ggccgaaccg gagacgctgt tctccaccgc cggcaccatc acgcgcaggc
     1141 gcacgggaag cttcgcgtcc atgatggtcc gggcgaggcc aagcacgcag gcagcacccc
     1201 ccatgtcttt cttcatggtc gccatgccgg aggcgttctt caaatcgagt ccgcccgtgt
     1261 cgaaacagac acctttgccg acgagcgtca ccttgggatg ggagctatcg ccccagacga
     1321 ggtcgatgag ccggggggtg cgcacgctgg cgcggcccac cgcatggatc atcggataat
     1381 tcgcagcgag caaggcatcg cccgccgtca cgctaagccg cgcgccgaaa ctttccccaa
     1441 gcgaacgcgc cgcctgttcc aattcgtccg ggcccatgtc gttggagggg gtgttgatga
     1501 ggtcgcggcc caggaacacg ccttgagcga cgcgcgatat cgtctcgcca tcgacacctg
     1561 cgggcagcac cagacgcggc ggttcgcggc ggctggattt cttgtgcttg cggtaacggt
     1621 cgaaggcata ggtgccgatg gcccacgcat aggccgcgcg cgccccggaa aaagcgtccg
     1681 gcacatcgcc cagcgtatag agaccctgcg gcaaacgttc ggagaacagc gccagcgcat
     1741 ggggatcgcg tcccgtgccc aagccaagca ccgcctcgcc gagggcgccc gtttctgcgg
     1801 gcaccaggac aagttcgcct tccttggcgg agaatcccgt tgctttcagc cagcgtgctt
     1861 gggccggctc acgcgcgccg aggaatttct cgagatcctt gggaagcacc gcatgcagca
     1921 tgaccggcgt gaccgcaccg gcgtcggtct ggttcacgaa aatgctcaac atgaaaggtt
     1981 tgaatctccc ggctgaagcc gctcttataa gagcggtaag acgtctttcg caatcggcaa
     2041 ccgaaaccca agatgaccgc gcgctatacg cttttcatcg gcagcaagaa cacatcgtcc
     2101 tggagtctaa gaccgtggct cgccatgaaa atggcgaaac tcccattcga ggacgtgctg
     2161 atcgtactcc gccagcccga gaccaaggaa cagatcaaac cgcattcgcc atcgggcaag
     2221 gtgccggcac tgcgaatcgc ggacggggcc accgtggaaa ccgtttggga cagccttgcg
     2281 atctgcgaga cactggccga acgccatccc gatgcgcgga tgtggccgca ggatgcagcc
     2341 gcacgggcgc acgcccgcag catcgccgcc gaaatgcatt cgggctttgc cgatgtgcgc
     2401 aatgtccttc ccatggacat cgccacacgc catccgacac ccaggctcga tgattccacg
     2461 cagacgcaga tcgcgcgcat tgtcgcggtc tgggaagaga cgctttcgca ttacggaaag
     2521 gacggcgggt tcctcttcgg tccattcacg atcgccgatg ccttctacgc gcccgtggcc
     2581 acgcgcttcg agacctatgg catcgccttg ccgcccgcgg cgcaggccta cgcgcaacgt
     2641 gtcctgtcgc tcggtccgat gcgcgaatgg gaagcggcgg caagagcgga aaaaacggca
     2701 agcccgtcgg agcggccata acagccgggc attatcaggt tcttccggag ccggaccttg
     2761 cgggaagcgc agccttttgc gctggtttca acaggcccgc ttcggtgaaa aaccgcgccg
     2821 cgccgggatg aagcggggcc attccggctt ttgcgccgta agtctcgtcc aggaaatgcg
     2881 ttcccagttt tccggctgtc aaggtcgcgc ggttggccgg attgtacagc gccttcagca
     2941 ttccgtagat cagtgcgtca ggttcggatg catccgtgat ccatagcgca tccacgctga
     3001 ccgtctcgag atcgggcgtg ttggcgtaag tgccctttgc gatcacatgc ggcgagagaa
     3061 acgattcctt cgcgaggaga cgcttcaaac cgtcgccatc gatcggcacc aggacagcga
     3121 catcttcgtc cagcaatggc tgcaagagat tgaccggcgt gccgccgaca aagaacagcg
     3181 cgtcgagctt tcccgcctgt agcaggtcga cagccttttc ggcggaatcg taattcgcaa
     3241 tgatggattt ctccgtgagc cggtaggcgg ccagcaccgc gcgcgcggtc acgatggtgc
     3301 ccgagccttc gctcgacaac gaaacgcgct tgccgcgcag atcggcaacc gatttgatct
     3361 tcgcgccctt ggccgcgatc agatgcaagt cctcgccata gagattggcg atgacacgaa
     3421 cgtccttcgt ggggccggct ttgcggaacg gaccctgccc cgccatcgcg agggcgatca
     3481 cgtcggtctg ggccaggccc gatgtcacca tcccgctatt caccgcaagc acgttggcaa
     3541 ttgagccctg agacgcacgg gtggacacga tgagacccga cggcccgcaa atgttcgcgg
     3601 cctcgcaacg tccgacgccg ggcggatggc tcagaagctg agccagcatc gaaccgatgg
     3661 ggaaataggt tcctgtcgtg gagccggtag agatctggaa ggcgatccgc tgcgggttgt
     3721 cggtgatttg ggcggtggcc gcgatggcga gcgtcagcga cacgccaaga gcgaagatcg
     3781 ctccggtcgc aaggctccaa gagactcgtc tcttcgcttg catgacgctg tatagaacag
     3841 acccatt
//
LOCUS       DASYEG010000032         2503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2185206, whole genome shotgun sequence.
ACCESSION   DASYEG010000032 DASYEG010000000
VERSION     DASYEG010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2185206"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VGM72_01345"
     CDS             complement(<1..562)
                     /locus_tag="VGM72_01345"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263936.1"
                     /translation="MAFTSWRGVVGNIKPTMRPGSVEELCRILPEGVGIIPLFLDIQK
                     GAREEFTRIIPHYEPLIAKLAEHEVDLIHPEGAPPFMVLGYEGEAELIKKWEKKYKTP
                     IFTSGTNHIRALNALKIKRFVGASYFSGKINETFGRYFVDAGFDVLGMEGIDVPFHDV
                     GNLSGELVYAHVKKIFLKHRKEAEGIY"
     gene            722..>2503
                     /locus_tag="VGM72_01350"
     CDS             722..>2503
                     /locus_tag="VGM72_01350"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY4263937.1"
                     /translation="MGRYRLTVDTGGTFSDFVYWNEDTGEIFVAKLPSTPDDPSRAIL
                     AGVETLLARGVAAADIGYFCHGTTVGTNALLEGKGVRTGLLVTKGFRGIYEVAEQCRP
                     HGSAIFDIFYDRPPLLVPESLTGEVVERITATGDVLLALDEAALRRTVRELRGQDPQA
                     IAVCLLFSFLAPEHEERARAIIAEEIPGCAVSLSSDIVPQIREYYRLSTTVINAYLEP
                     ILARYIGNLEERLAGAGLTTRQTYIMQSNGGMATFAASAKKAVATVLSGPAGGITASV
                     QTCRTTGLSNLITFDMGGTSCDVALIKDGEAAVQNRGKIEGRDISLPMIDINTVSAGG
                     GTLARVDRFGQLEVGPQSAGAVPGPACYGRGGAEPTITDCNVVLGYLGADNFLGGKMR
                     LDIEAACRVVETIARPLKLGVADAAEGIVRIIDVKMQEAIKAISTMRGHDLRDFHLLA
                     FGGAGPLHAARIAAELGMAGVVVPLYPGVYAAMGLLMSDVKHDYIRSKMAGLAKVGPA
                     DIGAVFAELESHAMRDLRDEGFAPARIGIERALDLRYAGQGYEITIACDYPLGDGSIA
                     SLRARFDETHRQMFGHTAPEEPVEIVSY"
BASE COUNT          473 a          763 c          783 g          484 t
ORIGIN      
        1 gataaattcc ttccgcttcc ttgcggtgct tgaggaagat cttcttcaca tgggcataga
       61 ccagttcgcc cgacagattg ccgacatcgt ggaacggcac atcgatccct tccatgccca
      121 gaacatcgaa acccgcatcg acgaaatagc gtccaaaggt ttcgttgatc ttgcccgaaa
      181 aataggaggc gccgacaaag cgctttatct tcagcgcgtt cagcgcccgg atatgattcg
      241 tgcccgaggt gaagatgggg gtcttatact tcttctccca tttcttgatc aactcggcct
      301 cgccctcata gccgagcacc atgaagggcg gcgccccttc gggatggatc aaatccacct
      361 cgtgttccgc gagcttggcg atcaacggtt cataatgcgg gatgatgcgc gtgaattcct
      421 cacgcgcgcc cttctggatg tcgaggaaga ggggaataat ccccacgccc tcgggcagga
      481 tgcggcacaa ttcctcaacg gagccggggc gcatggtcgg cttaatgttg ccgacgacgc
      541 cgcgccagga tgtgaaggcc aaattcgtcc ccttttcgga ttcgtataaa agctggccga
      601 aattaggagg tttgaggggc ggtttccagg cgctttctta agtgcgcgtc aacggccttg
      661 ttgccccatt gttccttcta taaaccgctc cccacacagg caatcgcatc agaaagccgc
      721 tatgggccgc tatcgtctga ccgtggatac cggaggcacg ttctccgatt tcgtctattg
      781 gaacgaagac acgggcgaga tcttcgtcgc caagctgccg tccacgcccg acgatccgtc
      841 gcgcgcgatc ctggcgggcg tggaaacact gctcgcccgc ggcgtggcgg cagccgatat
      901 cggttatttc tgtcatggga ccacggtcgg caccaatgcg ctcctggaag gcaagggcgt
      961 gcgcaccggc cttctggtga ccaaggggtt tcgcggcatt tacgaagtgg cggagcaatg
     1021 ccggccgcat ggttcggcaa tcttcgatat tttctacgac cgcccgccgc tgctggtgcc
     1081 cgaaagcctg accggcgaag tggtggaacg catcaccgcc acaggcgacg tgctgctcgc
     1141 gctcgacgag gccgcgttgc gcagaacggt acgcgagctt cgcggccaag accctcaggc
     1201 gattgccgtg tgcctgctgt tttcgtttct ggcgcccgag catgaggaac gcgcccgcgc
     1261 catcatcgcc gaggaaatcc cgggatgcgc ggtatcgctg tcctccgaca tcgtgccgca
     1321 gatccgcgaa tattaccggc tgtcgaccac ggtgattaac gcctatctcg aaccgatcct
     1381 ggcgcgctac atcggcaatc tggaggagag gctcgccgga gcagggctga cgacgcggca
     1441 gacatacatc atgcaatcca atggcggcat ggcgaccttc gcggcgagcg cgaaaaaggc
     1501 ggtggcgacg gtactttccg gaccggcggg cggcatcacc gccagcgtgc agacctgccg
     1561 caccaccggg ctttccaacc tcatcacttt cgacatgggc ggcacgtcct gcgatgtcgc
     1621 gctcatcaag gatggcgagg cggcggtgca gaaccgcggc aagatcgaag ggcgcgatat
     1681 ttcgctcccc atgatcgaca tcaatacggt gagcgcgggc ggcggcacgc tcgcgcgcgt
     1741 ggaccggttc gggcagctcg aagtcgggcc gcaaagcgcg ggcgccgtgc ccggacctgc
     1801 ctgctatggt cgtggcggcg ccgagcccac catcaccgat tgcaatgtcg tgctcggtta
     1861 tctgggcgcg gacaatttcc tgggcggcaa gatgcggctc gacatcgagg cggcctgtcg
     1921 cgtggtagag accatcgcgc gcccgctgaa gctcggcgta gcggatgccg ccgaaggcat
     1981 cgtgcgcatc atcgatgtga agatgcagga agcgatcaag gcgatctcca ccatgcgcgg
     2041 gcacgatctg cgcgatttcc atctcctggc cttcggcgga gcggggccgc tgcatgccgc
     2101 acgcattgcc gccgaactcg gcatggcggg cgtggtggtg ccgctttatc ccggcgtcta
     2161 tgcggcgatg ggtcttttga tgtcggacgt gaagcacgat tacatccgct cgaagatggc
     2221 tggactcgcc aaggtggggc ccgcggacat cggtgcggtt ttcgccgaac tcgaaagtca
     2281 cgcgatgcgc gatttgcgcg acgagggctt cgcacccgcg agaatcggga tcgaacgcgc
     2341 gctcgacctg cgctatgcgg gtcagggcta tgagatcacg attgcgtgcg attatccgct
     2401 tggtgacggc agcatcgcaa gtctacgcgc gcgcttcgat gaaacgcaca gacagatgtt
     2461 cggtcacacc gcgcccgagg agccggtcga aatcgtctcc tat
//
LOCUS       DASYEG010000033         9920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2765778, whole genome shotgun sequence.
ACCESSION   DASYEG010000033 DASYEG010000000
VERSION     DASYEG010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 9920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9920
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2765778"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VGM72_01355"
     CDS             <1..241
                     /locus_tag="VGM72_01355"
                     /inference="COORDINATES: protein motif:HMM:NF013216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY4263938.1"
                     /translation="WTLSRIDSILRALLRSATYELVARRDVPAKVVIDEYVELARDFF
                     EGEEPGFVNAVLDRLAHRKRAAEFGETPPDDELQF"
     gene            222..1226
                     /gene="thiL"
                     /locus_tag="VGM72_01360"
     CDS             222..1226
                     /gene="thiL"
                     /locus_tag="VGM72_01360"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEY4263939.1"
                     /translation="MTNSSSEPTQRLGEFAMIAKLFAPLAANAPGAFGLTDDAATLGL
                     APGEELVVTVDALVEGVHFFKDDPAAAIAKKALRVNLSDLAAKGARPCGYLLALSLPP
                     WIGDEWLAQFAAGLGEDQEKFGIDLLGGDTTSTPGPLTLSITALGSVPSGRMLRRAGA
                     MPGNRVFVSGTIGDAGAGLDLLQQANREVAAPDRAGLIARYRLPEPRLALGGHLIGLA
                     TAAADVSDGLIADLGHIAETSKVRIVIDAARIPLSPALARLWGREGEAVLRAATAGDD
                     YEIVFTAPQTAAAGIAKAAQDTGIPVAEIGRVETGQGAALLDENGDCLPIPRSGFTHF
                     "
     gene            1284..1712
                     /locus_tag="VGM72_01365"
     CDS             1284..1712
                     /locus_tag="VGM72_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263940.1"
                     /translation="MTTPVKTFAALAVALLFFLPLQDATAAEAAKDAGPTDVEMPAML
                     APMVVDNRLESYAYITVALTPASRDKTLLIREKVPFLRDGFLREVNKASIVKPAEPTA
                     VDQPALKKRLLARVNQILPQGTVSDLKFQEIVMTPIQPQS"
     gene            1872..4010
                     /locus_tag="VGM72_01370"
     CDS             1872..4010
                     /locus_tag="VGM72_01370"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001951.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEY4263941.1"
                     /translation="MSLPLTLVLACGVIALAYGGWLVQSVLAQSAGTKEMQEIAAAIQ
                     EGAAAYLNRQYTTIAIAGAVIFVAAFIVLGWQVAVGFLIGSTLSGAAGYIGMNVSVRA
                     NVRTAEASRHGLAEGLSVAFRSGAVTGLLVVGLGLLGVAGYYAVLTGAFGLSVTDPAQ
                     SRVVVNALVALGFGASLISIFARLGGGIFTKGADVGGDMVGKVEAGIPEDDPRNPATI
                     ADNVGDNVGDCAGMAADLFETYAVTTVATMVLASIFFADAERIAMMVYPLTIAGACIL
                     TSIVGTFFVRLGPSRNIMGALYKGLIATGVLSLAVLLPLTYWIVGLDTQYAILGGARF
                     TGWHLYFCGVAGLVVTGLLVWITEYYTGTDFRPVQSVAKASQTGHGTNVIQGLAVSME
                     STALPALVICVGIVVTYLLAGLFGIAIAVTTMLALAGMIVALDAFGPVTDNAGGIAEM
                     AGLEGDVRKTTDALDAVGNTTKAVTKGYAIGSAGLGALVLFAAYTEDINYFVANGDSY
                     PYFKGLELPTFALTDPWVVVGLFIGGLLPFLFGGMAMTAVGRAAGAVVEEVRRQFREM
                     PGIMLRTQKPDYGRAVDMLTKAAIREMILPSMLPVLSPIVLFLIVRGIAGPTSALNAL
                     GAMLLGVIVTGLFVAISMTSGGGAWDNAKKYIEDGHYGGKGSDAHKAAVTGDTVGDPY
                     KDTAGPAVNPMIKITNIVALLLLAVLAHLA"
     gene            complement(4084..4533)
                     /locus_tag="VGM72_01375"
     CDS             complement(4084..4533)
                     /locus_tag="VGM72_01375"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEY4263942.1"
                     /translation="MLAACAAVENQRGYVPDMQALSSIEVGMDTKDSVSKKLGDPSTA
                     ATFGNDIWYYMSSHVEQNAFFAARATDRNIVAVEFAKDGKVTNVHKYTLADGHVVDFV
                     SRETPTRGRELSLIQQIFNAVPGQIGQSAPTDAIPGGGMPPPIPAPR"
     gene            4674..5201
                     /locus_tag="VGM72_01380"
     CDS             4674..5201
                     /locus_tag="VGM72_01380"
                     /inference="COORDINATES: protein motif:HMM:NF015914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HEY4263943.1"
                     /translation="MLRRFLGSDKSAAAARVLYAGLVVRARAPVFHTAFQVPDTIDGR
                     FDLLALHGFVVMEALKPLRRPGRSLGTALASVIFAGFDDALRELGVSDFGIPRRIKAM
                     AGAFYGRLEAYGAADSQRALGEAILRNLYRAEATRRHEADVLAHYIALAREALRAQAD
                     VLLQGHADFGPLPKI"
     gene            5211..5825
                     /locus_tag="VGM72_01385"
     CDS             5211..5825
                     /locus_tag="VGM72_01385"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEY4263944.1"
                     /translation="MVFAAPYSLVFDLGLVPKLGTQIVLDPTQAERAAISGWLGIEDL
                     ESLTAMLLISRDGENHYSCAANFEADVVQACVVTLEPVRAHLSGTYHRLFQVLPRSSS
                     RRKNPVGEPPSLDVPLAGEDGPELLDNSVVDLAAPLLEELSLALDPYPRAPGVSFAPP
                     VEEVEPADNPFAVLEQLKAPPSPPARSVKPEIGTKPASAGRKRR"
     gene            5997..6176
                     /gene="rpmF"
                     /locus_tag="VGM72_01390"
     CDS             5997..6176
                     /gene="rpmF"
                     /locus_tag="VGM72_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEY4263945.1"
                     /translation="MAVPKRKTSPSRRNMRRSHHALSPNAHVECANCGELKRPHHVCA
                     ACGHYDGREVVAAEA"
     gene            6267..7340
                     /gene="plsX"
                     /locus_tag="VGM72_01395"
     CDS             6267..7340
                     /gene="plsX"
                     /locus_tag="VGM72_01395"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEY4263946.1"
                     /translation="MILSVDAMGGDSGPASVLAGVAIARRRHPNVRFLLHGDEAVLAP
                     LLTRHRALAAHAEIRHAPAVIGMAEKPSQAIRRGRNSSMWHALEAVARGDAQVAVSAG
                     NTGALMAISMFRIGLIEGVSRPAIAVLWPTVRGQSVVLDVGANAGSDAEQLVDFAVMG
                     EAFARAIFGIERPTVGLLNIGEEEVKGIESVKQAAQILRASKLPIEFHGFIEGDDIGA
                     GLVDVVVTDGFTGNVALKTAEGTAKLILFYLRSALKRSLLGRLGALLASGAFRVLKRK
                     LDPRNLNGGVFLGLNGVVVKSHGGADALGFASAIDLAIDMGGSDMIGRILTDRAAISS
                     SPEQPGAASGQPNSGNTAKAAVS"
     gene            7337..8314
                     /locus_tag="VGM72_01400"
     CDS             7337..8314
                     /locus_tag="VGM72_01400"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY4263947.1"
                     /translation="MTSIRSVVRGCGAFLPANVVSNDDLAKRVDTSDTWVVERTGIRR
                     RHIAAAGEKTSDLALAAGTRALENARLSAADLDLVIVATTTPDETFPSVATKVQAGLG
                     MVRGAAFDLQAVCSGFIYGLSVADNFIRGGQAKTVLVIGAETMSRILDWNDRTTCVLF
                     GDGAGAVVLQAAEGEGTNADRGILNTQIFSDGRLHDLLYTDGGVSTTGTAGYLRMHGR
                     EVFKHAVTNISAAIEASTRRAGITPADIDWFVPHQANQRILDGTARKLNIRPERVIST
                     IADHGNTSAASVPLALSVGVEDGRIKRGDLVLMEAMGGGFTWGAALLRW"
     gene            8432..8752
                     /locus_tag="VGM72_01405"
     CDS             8432..8752
                     /locus_tag="VGM72_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008755922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEY4263948.1"
                     /translation="MGTRTLTRADLTEAVFNAVGLSRNESSQMVEDILNEVCGALSRG
                     ETVKLSSFGTFQVRQKSQRVGRNPKTGDEVPIAPRRVLVFRPSHVLKAQINGLDSVGL
                     HDEE"
     gene            8757..9257
                     /locus_tag="VGM72_01410"
     CDS             8757..9257
                     /locus_tag="VGM72_01410"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY4263949.1"
                     /translation="MSVLSAQKSPEAFRTISEVAMELDVPQHVLRFWETRFANIKPLK
                     RGGGRRYYRPDDLDLLRGIRALLYRDGLTIKGVQKILREQGARYVMELGRGTVSLITV
                     SPKATAAPAASKADGRSNVHPFSRESGGAPGLSKAERARFSMLIDELLDLKTRLSFAR
                     QMQASR"
     gene            9392..9469
                     /locus_tag="VGM72_01415"
     tRNA            9392..9469
                     /locus_tag="VGM72_01415"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9427..9429,aa:Pro,seq:ggg)
BASE COUNT         1683 a         3019 c         3175 g         2043 t
ORIGIN      
        1 ctggacgctg agccggatcg attcgatttt gcgggcgctt ttacgaagcg cgacctatga
       61 gttggtggcg cggcgcgatg tgccggccaa ggtggtgatc gacgaatatg tcgaacttgc
      121 ccgagacttc ttcgaaggcg aggagcctgg attcgtgaac gccgtgctgg accgcctcgc
      181 gcaccgcaaa cgggcggccg aattcgggga gactccgccc gatgacgaac tccagttctg
      241 aaccgacgca acgcttgggc gaattcgcga tgatcgcgaa attatttgcg ccgctggcgg
      301 ccaatgcccc tggtgcgttc ggtctcaccg atgatgcggc aacgctcggt cttgcgcccg
      361 gcgaggagct tgtggtcaca gtcgacgcac tggtcgaggg cgtccatttc ttcaaagacg
      421 atcccgcggc cgcaatcgcc aaaaaagcgc tgcgcgtgaa tctttccgat ctggccgcca
      481 aaggggcacg gccatgcgga tatcttctgg cattgtcgct gccgccttgg atcggtgacg
      541 aatggctcgc gcaatttgcc gcgggtcttg gcgaggacca ggagaaattc ggcatcgatc
      601 ttttgggcgg cgataccacg tccacaccgg gaccgctgac gctttcgatc accgcgctcg
      661 gaagcgtgcc ctcgggaagg atgctgcggc gggcgggcgc catgccgggg aatcgcgtgt
      721 tcgtttcggg cacgatcggg gatgcgggtg cgggccttga cctactgcaa caggcaaaca
      781 gagaggtcgc ggcgccggat cgcgccggac tgatcgcacg ctatcggctg cccgagccac
      841 gcctcgcttt gggcggccat ttgatcggcc ttgccaccgc cgcggccgat gtctccgacg
      901 ggctgatcgc cgatcttggc catatcgcgg aaacctctaa ggtccggatt gtcatcgacg
      961 cggcgcgcat accgctttcg cctgcgcttg cccgtctttg gggacgggaa ggggaagccg
     1021 tgctgcgcgc cgccaccgcc ggcgacgatt atgagatcgt cttcacggcc ccgcaaaccg
     1081 ccgcagccgg gatcgcaaag gcagcccagg acacgggaat cccggtggcc gagatcggcc
     1141 gcgtcgaaac ggggcagggg gccgcccttc ttgatgaaaa cggcgattgt cttcccatcc
     1201 cgcggtctgg tttcacccat ttctgacccg cggacggcag ggcggtaacc aagccttagt
     1261 acggtttcgg gcactctcat cccatgacga cgcctgttaa gacatttgcg gctctcgcgg
     1321 tcgccttgtt gttctttctg ccgctccagg atgcaacggc ggctgaggcg gccaaggacg
     1381 caggtcccac cgatgtggag atgccggcca tgctggcgcc catggtcgtg gacaaccgcc
     1441 tggaaagcta tgcctacatc accgtcgcac tgacaccggc gtcccgcgac aaaaccctcc
     1501 tcatccgcga gaaggttccg ttcctgcgcg atggcttcct cagggaggtc aacaaggcca
     1561 gcatcgtcaa gccggccgag cctacggccg tggaccagcc cgcgctgaaa aagcggcttc
     1621 ttgcccgcgt gaaccagatc ctgccccagg gcaccgtcag cgacctcaaa ttccaggaga
     1681 tcgtcatgac gcccattcag ccgcagtcct gaacttaagc aattcgagtc tttttgttgc
     1741 gcgctgcgca tgactttggc atgttccgcc ccccatgcgg cggggtgggg gcttgccgca
     1801 tctctcatct tgtgagaggc aagccgcgtt tcccctccct taccggaatc gacacatata
     1861 aggactgcaa gatgagcctg cctctaaccc tcgttctcgc ctgcggcgtg attgcgctcg
     1921 cctatggagg ctggcttgtg cagtcggttc tggcccagag tgcgggcaca aaggagatgc
     1981 aggagattgc cgcggcgatt caggaagggg ccgccgccta tctgaaccgt cagtacacga
     2041 ccattgcgat cgccggtgcc gtcatcttcg ttgccgcatt catcgtgttg ggctggcagg
     2101 tggccgtcgg ctttctgatc ggctccacgc tttcgggcgc tgccggctat atcggcatga
     2161 atgtctcggt gcgcgccaat gtgcgcaccg ccgaagcctc gcgccacgga ctcgccgaag
     2221 gattgtctgt cgcattccgc tcaggcgcgg tgaccggtct gctggttgtc ggtctcggac
     2281 ttctgggtgt ggcgggctat tatgcggtcc tgaccggagc gttcggcctt tcggtcaccg
     2341 atcccgctca aagccgcgtg gtggtgaacg ccttggtggc gctgggcttc ggcgcctccc
     2401 tgatctcgat tttcgcccgt ctcggcggcg gcatcttcac caagggtgcg gatgtcggcg
     2461 gcgacatggt gggcaaggtg gaagccggta tcccggaaga cgatccgcgc aatccggcca
     2521 ccattgccga caatgtcggc gataacgtcg gagactgcgc cggcatggcg gccgatctgt
     2581 tcgaaaccta cgcggtgacg actgtcgcca ccatggtgct ggcctcgatc ttctttgccg
     2641 atgccgagcg catcgccatg atggtctatc cgttgaccat cgcgggcgcc tgcatcctga
     2701 cctcgatcgt ggggacgttt ttcgtacggc tcggccccag ccgcaacatc atgggcgcgc
     2761 tctataaggg cctgatcgcc acgggcgtgt tgtccttggc cgtgctcttg cctttgacct
     2821 actggatcgt cggtctggac acccaatatg ccattctcgg cggcgcgcgt ttcaccggct
     2881 ggcatctcta tttctgcggc gtcgcgggtc tggtcgtgac ggggcttctg gtctggatca
     2941 ctgaatatta tacgggcacg gattttcgcc ccgtacagtc ggtggcgaag gcctcgcaaa
     3001 ccggccatgg caccaacgtc attcagggac tggcggtctc aatggaatcg accgcgctgc
     3061 ccgcgcttgt catctgcgtc gggatcgtcg tgacttatct tctggcgggc cttttcggca
     3121 tcgcgatcgc ggtgacgacc atgctggcgc ttgccggcat gatcgtggcg ctcgatgcct
     3181 tcggtccggt gaccgataat gccggcggca ttgccgagat ggccgggctg gaaggcgatg
     3241 tccgcaagac gacggatgct ctcgatgccg tcggcaatac gacgaaggcg gtgacgaaag
     3301 gctatgccat cggttcggcg ggcttgggcg ccctcgtgct gtttgcagcg tatacggagg
     3361 atatcaacta tttcgtcgcc aatggcgatt cctatccgta tttcaaaggg ctggagcttc
     3421 ccaccttcgc gctcaccgat ccctgggtcg tcgtgggact cttcatcggc gggctgctgc
     3481 cgtttttgtt cggcggcatg gccatgactg cggtcggacg cgcggccggc gcggtggtgg
     3541 aagaggtgcg ccgtcagttc cgggaaatgc cgggcattat gctgcggacc cagaagcccg
     3601 attatggccg cgcggtggac atgctgacaa aagccgcgat ccgggagatg atccttccct
     3661 cgatgctgcc cgtgctgtcg cctatcgtgc tgttcctgat tgtgcgcggc atcgccggcc
     3721 cgacctcggc gctcaatgcg ctgggggcca tgctgctggg tgtcattgta acgggcctgt
     3781 tcgtggccat ctcgatgacc tcagggggag gcgcttggga caacgccaag aaatacatcg
     3841 aggacggtca ttatggcggc aagggttcgg acgcacataa ggcggcggtg accggcgaca
     3901 cggtcggtga tccctacaag gacaccgccg gaccagccgt gaatccgatg atcaagatca
     3961 ccaacattgt ggcgctgctg ctgctcgcgg tgctggcgca cctcgcataa agcacaattg
     4021 cgcggcaaaa tccgcgcctg gagtgacact cgcttctatc cgaggtgccg ctctatgcgc
     4081 gtttcagcgc ggtgccggta tcggcggagg catgccgccg ccggggatgg cgtcggtcgg
     4141 cgccgattgg ccgatctgtc ctggcaccgc attgaagatt tgttggatca ggctcagttc
     4201 gcggccgcgc gtgggcgtct cccggctgac gaaatcgacc acgtgcccat cggccagcgt
     4261 gtatttgtgg acgttcgtca ccttgccgtc cttggcaaat tcgaccgcca caatgttgcg
     4321 gtcggtggcg cgcgccgcga agaaggcgtt ctgttcgaca tgcgatgaca tataatacca
     4381 gatgtcattg ccgaaggtgg cggccgtgga gggatcgccg agctttttgc tgacgctgtc
     4441 cttggtgtcc atgccgactt cgatgctcga cagcgcctgc atgtcgggga cgtagccgcg
     4501 ctgattttcc accgccgcgc aggccgccaa catggttgcc ccggcaacac atgaccatct
     4561 caaaaggcgc atcgcactga actccctcga ctgaccgccc cgcaggttcc caatatctaa
     4621 tgtgctagct tcctcttaga tgtcttgcaa gtccagcacg ctacggcccc ttcatgctcc
     4681 gccgatttct tggttccgat aaatccgccg ccgccgcgcg ggtcctgtat gcgggcctag
     4741 tagtccgggc gcgcgcgccg gttttccaca ccgcattcca agtgccggac acgatcgatg
     4801 gccgtttcga cctgctggcg ttgcatggct ttgtggtcat ggaggcgctg aaaccattgc
     4861 ggcgcccggg ccgcagcctg ggcaccgcgc tcgcaagcgt catctttgcc ggtttcgacg
     4921 atgcgctgcg cgagcttggc gtcagcgatt tcggcattcc ccgccggatc aaagccatgg
     4981 cgggtgcctt ctatggcaga ctcgaagcat atggcgcagc agattcgcag cgcgcgcttg
     5041 gggaagcgat cctgcgcaat ttataccgcg cggaagccac gcgccggcat gaagccgacg
     5101 tcctggcgca ctatattgcc ctggcgcgcg aggccctgcg tgcgcaagcg gatgttttgc
     5161 ttcaaggtca tgccgatttc ggtccattgc ccaagatttg agttcacgcc atggtctttg
     5221 ccgctcccta ttctctggtc ttcgatctcg gcctcgtgcc gaaattagga acgcagatcg
     5281 ttctcgaccc cacgcaggcc gaacgcgccg ccatttccgg ttggctggga atcgaggacc
     5341 tggaaagcct tactgccatg ctcctgatct ctcgcgatgg tgagaatcat tattcctgtg
     5401 ccgcgaattt cgaggccgat gtggtccagg cctgcgtggt gacacttgag ccggtccgcg
     5461 cccatctgtc gggaacctat caccgccttt tccaggttct tccccgatcc tcgtccagac
     5521 gaaagaaccc ggttggagag cccccgagcc tggatgtgcc gttggccggc gaggatgggc
     5581 cggaattgct ggacaactct gtcgtggacc tcgcggcgcc tctgcttgag gagcttagcc
     5641 ttgcgcttga cccttatccc cgggcgcccg gggtcagctt cgcccccccg gtcgaggagg
     5701 tggaacccgc ggataaccct tttgccgtgc tggaacagct caaagccccc ccatcgcctc
     5761 cggcccggtc cgtgaagcct gaaatcggaa caaaaccggc ttccgctgga agaaaacggc
     5821 gctgagaagg atacggcctg gcgccgattg cttaagaagc gtccctatgc gtacggattg
     5881 aacgatccct tgcgtcggct gcattttcgg gttacctccg cccctgtttt ccgtttgtga
     5941 ccgtgcgggt ccggacggca gaaccgcgtg catttgttca ggaaagggtt tccgccatgg
     6001 cggttccaaa acgaaaaacg tctccgtcgc ggcgcaatat gcgccgttcg catcatgcgc
     6061 tttcgccgaa cgcacatgtc gaatgtgcca attgcggcga gttgaagcgc ccgcaccatg
     6121 tttgcgccgc ctgcggccat tatgacggtc gcgaggttgt tgccgctgag gcgtgaaccc
     6181 ggttctatct gggctttgag ggcccgcacc cttaagcttg cattcgtagc tgcggatgtt
     6241 cgggggctgc taacgtggtc cgggacttga ttctgtctgt cgacgccatg ggtggcgatt
     6301 cgggacctgc ctccgttctg gcgggtgtcg cgatcgcgcg ccggcgtcat cccaatgtcc
     6361 gttttttgct tcatggcgac gaggccgtgc tggcgccgct cttgacgcgc catcgcgcat
     6421 tggcggccca tgccgaaatc cgtcacgcgc ccgccgtgat cggcatggcg gaaaagccgt
     6481 cccaggccat ccgccgcggg cgcaattcca gcatgtggca cgcgctggag gcggtcgcgc
     6541 gcggcgatgc gcaggtggcg gtctccgccg gcaacactgg cgcgctgatg gcgatctcca
     6601 tgttccggat cgggctgatc gaaggcgttt cgcggcctgc gatcgcggtt ctgtggccga
     6661 cggtccgagg gcagagcgtc gttctcgatg tcggcgccaa tgccgggagc gacgcggagc
     6721 aattggtgga ttttgccgtg atgggcgaag ctttcgcgcg cgcgatcttc gggatcgagc
     6781 ggcccacggt cggacttctg aatatcggcg aggaagaagt gaagggcata gaatcggtga
     6841 agcaggccgc ccagattctg cgcgcctcga aacttccgat cgaattccac ggcttcatcg
     6901 aaggcgacga tatcggcgcc gggcttgtcg atgtcgtcgt gaccgatggg ttcaccggca
     6961 atgtcgcgct gaagaccgcg gaaggcaccg cgaagctgat ccttttttat ctgcgctcgg
     7021 cactcaaacg ctcgttgctg ggccggcttg gtgcgctgct cgcgagcggt gctttccggg
     7081 tcctgaaacg caagctcgat ccccgcaatc tcaatggcgg cgtgttcctc ggtctcaacg
     7141 gtgtggtggt gaaaagtcat ggcggcgcgg acgcgttggg tttcgcgagc gccatcgatc
     7201 tggccatcga catgggcggc tcggacatga tcgggcgcat cctcacagac cgcgccgcca
     7261 tttcgtcctc cccggaacag cccggcgcgg cttccggaca acccaatagc ggcaacacgg
     7321 ccaaggcggc agtttcgtga catctatccg ttctgttgtt cgtggctgcg gcgcctttct
     7381 tccagccaat gttgtaagca atgacgacct cgcgaagcgg gtcgacacca gcgatacctg
     7441 ggtcgtcgaa cgcaccggga tcaggcgccg gcacatagcg gcggcgggcg agaagacatc
     7501 cgatctcgca ttggcggcgg ggacgcgcgc gctggagaat gcacggctga gcgcggccga
     7561 tctcgatctg gtcatcgtcg ccaccaccac cccagacgag acatttccat ccgtcgccac
     7621 caaggtgcag gcggggctcg gcatggttcg cggggctgcg ttcgatctgc aggccgtgtg
     7681 ttcgggcttc atctacgggc tgtccgtggc agacaatttc atccgcggcg gccaggcaaa
     7741 aaccgtcctg gtgatcggcg ccgagaccat gtcgcggatc ctggactgga acgaccgcac
     7801 cacctgcgta ttgttcggtg acggcgcggg tgcggtggtg ctgcaggcgg ccgagggcga
     7861 gggcaccaat gcggatcgcg gcatcctcaa cacccagata ttctccgacg ggcggctgca
     7921 cgatcttctc tacacggatg ggggggtctc cacgaccggg actgccggct acctgcgcat
     7981 gcacgggcgc gaggttttca agcatgcggt gaccaacatt tccgcggcca tcgaggcatc
     8041 gacccggcgg gccggtatca cgcccgccga tatcgattgg ttcgtgcccc accaggccaa
     8101 tcaacgtatc ctggatggca ccgcgagaaa gctgaatatc cgcccggagc gcgtgatctc
     8161 gaccattgcc gaccatggca acacctcggc ggcctcggtg ccgctcgcgc tgtcggtggg
     8221 ggttgaggac ggccgcatca agcggggcga tctggtgctg atggaagcca tgggcggagg
     8281 cttcacctgg ggcgcggcgc ttttgcgctg gtagagccgt tttagccggt gcttcaagca
     8341 aagcccccaa gcctctgaca ttgacgggaa atgtgcctgc gcctacggtt tgtgtcctgc
     8401 gcgagaggag tcgcgcgcca ggggggcatc gatgggcaca cggactttga cacgcgccga
     8461 tctcacagaa gctgtcttca acgcggtcgg cttatcgcgc aacgaatcct cgcaaatggt
     8521 cgaggatatt ctgaatgagg tttgcggcgc gctttcgcgc ggcgagacgg tgaagctttc
     8581 gtcttttggt acgttccaag tgcgccagaa gtcgcagcgg gtgggacgca atcccaagac
     8641 cggtgatgaa gtgccgatcg cgccgcgccg cgttttggtc ttccgtccct cgcacgtgtt
     8701 gaaggctcag atcaacggcc ttgattccgt gggcctgcat gacgaggaat aggtaattgt
     8761 ccgtcctttc ggcacagaaa tcgccggaag cctttcgcac catcagcgaa gtggctatgg
     8821 agttggatgt gccccagcac gtcctgcggt tctgggaaac caggttcgcg aacatcaagc
     8881 cgctgaaacg cggtgggggg cggcgttatt accgccccga cgatctggat ttgctgcgcg
     8941 gcatccgcgc gttgctttat cgcgatgggc tcaccatcaa gggcgtacag aagatcctgc
     9001 gcgagcaggg ggcgcgttat gtgatggaat tgggccgcgg cacggtaagc ttaatcacag
     9061 tatctccaaa agcgactgcg gcgccggcag cctccaaggc cgatggacgt tccaatgtgc
     9121 atcccttctc gcgcgagagc ggtggggcgc cggggttgag caaggccgag cgtgcgcgtt
     9181 tttcgatgct gatcgatgag ttgctggatt tgaagacgag gctttccttc gcacggcaaa
     9241 tgcaggcctc gcggtagacg aaatccgtat ctcgaactgc tgagagattg gtttcccgaa
     9301 agcgaaggat tcgttgcggg ggcgaacgtg cgcgactata gtccgcctcc ctcgaagttc
     9361 gggccctgat acgagggctg agcattggaa acggagcgtg gcgcagcctg gttagcgcac
     9421 cagtctgggg gactgggggt cgagagttca aatctctccg ctccgaccag acaaataact
     9481 cagcgataac agcggtttat ttgtctttcg tcggttgaat aaatattttt ccggtgtgcg
     9541 ttaggccgtt acggtaagcg tctggtaagc gccgtgagcg ttttcgagct gctccgactc
     9601 cgcgccgtcg catgcggcga gcgccgtccg ctggccccgg ccggttagat gtagaggacg
     9661 agaaatgagg gcaggcggca tgcgcgagcc gagggcgcgt gcgcagtatc agcgccgcgc
     9721 tcttgcgcag cacgcgaacc gcgtcacctg actaaggaaa catggcccgg cacgtcaggc
     9781 gcgcgctggg ccatccgtct ttcatctagt gaaagaactt acggccctgc cgtggggacc
     9841 gccaaatcat atgaccctat tgggtacttt gtacgctgct caagctttca tacaatccga
     9901 ttggataccc cggcgcatga
//
LOCUS       DASYEG010000034         2976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3925258, whole genome shotgun sequence.
ACCESSION   DASYEG010000034 DASYEG010000000
VERSION     DASYEG010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3925258"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="VGM72_01420"
     CDS             <1..1170
                     /locus_tag="VGM72_01420"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEY4263950.1"
                     /translation="PNYARGQTVYVTDASRAVGVVASLISKETRASVITDVRSEYARI
                     IASHDTNRGPQRRLTLGQARANRFAPDWASYAPHAPQFIGVRNLDSYDLGELARYIDW
                     LPFFQAWEMKGRFPDILDDPVAGPTARALFADAQAMLKRIVEEKWLTARASFGFWPAE
                     SSGDDIVLYTDESRTKQLAILHTLRQQVARERETPNLALSDFVAPAGAGKEDYAGAFV
                     VTTGHGEEAIAARFREDKDDYNAILLSALADRLAEAFAERLHERVRKEFWGYAPDEAF
                     ANAELIAEAYRGIRPAPGYPAQPEHTEKGTIFRILDAEKGAGVTLTESFAMWPGSSVS
                     GLYIGHPDSRYFGVGKIERDQVEDYARRKNWTLAEAERWLGPILNYNPRKIIEAA"
     gene            1339..1716
                     /locus_tag="VGM72_01425"
     CDS             1339..1716
                     /locus_tag="VGM72_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263951.1"
                     /translation="MILVLVTALVIAGYFIQFAAQYAEGSLKPFGRYLSLWVFVLAAL
                     LIVAAALQPALRARGIGFGGPGFARGAAFARGPGFARGPGPGFFQGRRGGRRFERREA
                     PPAPADNSAAQAPAPQGTPPATP"
     gene            complement(1777..2472)
                     /gene="aqpZ"
                     /locus_tag="VGM72_01430"
     CDS             complement(1777..2472)
                     /gene="aqpZ"
                     /locus_tag="VGM72_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005418268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HEY4263952.1"
                     /translation="MDMSRRLAAEFIGTFWLVLGGCGAAVLAAGVPMVGIGYAGVALA
                     FGLTVLTGAYAFGHVSGAHFNPAVTFGLFVGGRIPAKDIIPYWLAQLAGGIAGAAVLY
                     AIASGAAGFDASAGFASNGYGEHSPQHYSLMAGLISEVAMTFMFLIVILGATHDNAPA
                     GFAPLAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGSWALEQLWAFWLAPLVGAG
                     LAGLVQRWMHPAR"
     gene            complement(2557..>2976)
                     /locus_tag="VGM72_01435"
     CDS             complement(2557..>2976)
                     /locus_tag="VGM72_01435"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEY4263953.1"
                     /translation="RAEAEPRQSPQQEGRLVFEFGVNDVREVNGKRQLEAAQSLAAAR
                     EILSAASRWKPVLMIGPPPGCDEPRNARVKNLSDRLAKLCEELCVPYFDSYAALMDAP
                     VWMPSCKSVDGTHPDAPGYTEWASRIDAWPAWRDWLP"
BASE COUNT          571 a          936 c          911 g          558 t
ORIGIN      
        1 cccaattatg cgcgcgggca gacggtgtat gtgaccgatg cttcccgtgc cgtcggcgtg
       61 gtggcgagtc tgatctccaa ggagacgcgc gcgtcggtta ttacggatgt gcgcagtgaa
      121 tatgcgcgca tcattgcctc ccacgacacc aatcgcggac ctcaacggcg cctgacgctg
      181 ggtcaagcgc gcgccaatcg tttcgcgccc gactgggcgt cctatgcgcc gcacgcgccg
      241 caattcatcg gggttcgcaa tctcgattct tacgatcttg gcgaactggc gcgctatatc
      301 gactggctgc ctttcttcca ggcctgggag atgaagggcc gcttccccga tatcctggac
      361 gatccggtgg cgggtcccac ggcgcgcgcg ttgttcgccg atgcgcaagc catgctcaaa
      421 cgcattgtcg aggaaaaatg gctgaccgcg cgggcatcct ttggattctg gccggcggaa
      481 tcctcgggcg acgatatcgt tctttacaca gatgagtcac gcacaaagca actcgccatc
      541 ctgcacacgc tgcgacagca ggttgcgcgc gagcgcgaaa cgcccaatct cgcgctgtcc
      601 gattttgtcg cgcccgccgg cgccggaaaa gaggattacg ccggggcctt cgtggtgacc
      661 accggtcatg gcgaggaggc catcgccgcg cgcttcaggg aagataagga cgactataac
      721 gccatcctgc tctccgcact cgccgaccgg ctggccgaag cctttgccga gcggctgcac
      781 gaacgcgtgc gcaaggaatt ctggggctat gcgccggacg aagcctttgc caatgcggag
      841 ctgatcgcgg aggcctatcg cggtatccgt cccgcgccgg gctatccggc gcaacctgaa
      901 catacggaaa agggaaccat tttccgtatc ctggacgcgg aaaaaggagc cggcgtcacc
      961 ctgaccgaaa gtttcgccat gtggccggga tcgtccgtat ccggacttta tatcggccac
     1021 cccgactccc gttatttcgg ggtcggcaag atcgagcgcg atcaggtcga agattacgcg
     1081 cggcgtaaga attggacgct ggccgaggcc gaacgctggc tcggtccgat cctgaactac
     1141 aatccgcgca agatcatcga agcggcgtag ccgcaacgtc cgttcggccc agcactttcg
     1201 agtaattcat cgaactgtaa tgctgtgcgc attatggcgc gacatctgcg tggcaggatg
     1261 aatggtgtgg agtttgattg cggcgctttg gccatcgacc ggagtccgct tagggaggca
     1321 catgacatga ttttgttcat gattttggtt ctcgttactg ctttggtgat tgccggttat
     1381 ttcatccaat tcgcggcgca atacgcggaa gggtctttga agccgttcgg acgatatcta
     1441 tcgctttggg ttttcgtgct tgcggcgctt ctgattgtcg cagccgcgct tcagccggca
     1501 ctcagggctc gcggcattgg cttcggtggt ccgggctttg cgcgcggcgc tgcttttgca
     1561 cgcggtcccg gttttgcgcg tggtccgggt ccgggattct tccagggcag acggggcgga
     1621 cggcgcttcg agagacgcga agcaccgccc gcgccggcgg acaattcggc agcgcaagcg
     1681 cctgcgccgc aaggcacgcc accagcgacg ccctgaacgg atagcgttcc acgtgggagg
     1741 ggcacagcgc tcctcccgcg tgttcattgg aaaaaatcag cgtgccggat gcatccagcg
     1801 ctgcacgagc cccgcgaggc ccgcgccaac cagcggcgcc agccagaacg cccaaagctg
     1861 ttcgagcgcc cagcttccct ggaagatcgc gaccgctgtc gaacgcgccg gattcaccga
     1921 cgtattggtc accggaatcg agatgagatg gatcaaggtc agcgcgaggc cgatggcgag
     1981 cggcgcgaag cccgcgggtg cgttgtcatg ggtcgcacca agaatgacga tcaggaacat
     2041 gaacgtcatc gccacttctg aaatcagccc ggccatcagc gaataatgct gcggcgaatg
     2101 ctcgccatac ccgttggacg caaaacctgc cgatgcatcg aacccggccg cgccgctggc
     2161 gattgcataa agcaccgcgg cgccggcgat gccccctgcc aattgcgcca gccaataagg
     2221 gataatgtct tttgccggga tgcgtccgcc cacgaacagg ccgaacgtca ccgccggatt
     2281 gaagtgggcg cccgagacgt gtccgaacgc gtaagccccg gtcagcaccg tcaatccaaa
     2341 tgcgagagcg actccggcat agccgatgcc gaccatcgga acgccggcag ccagcaccgc
     2401 cgctccgcat cccccgagga caagccagaa tgtgccgatg aattccgctg caagcctgcg
     2461 cgacatgtcc atgatgagcc ctcacccctg cgcttttcca accgccgagt tcaagcttac
     2521 atccacatct tcaccgggag tgagcgaaat tttggtctag ggtagccaat cccgccaagc
     2581 gggccaggca tcgatacggc tggcccattc cgtgtaaccc ggagcgtcag gatgcgtacc
     2641 atccacggat ttgcagctcg gcatccagac gggggcgtcc atgagcgccg cataggaatc
     2701 gaaatagggc acgcagagtt cttcgcacag ctttgcaagc cgatcggata gattttttac
     2761 ccgcgcgttg cggggctcat cgcaccccgg cggcgggccg atcatcaaaa ccggtttcca
     2821 gcgcgatgcc gcactcagga tttcacgcgc ggcggcgagc gattgggcgg cctccaattg
     2881 ccgtttgccg ttcacctcgc gcacatcgtt gacgccgaat tcaaaaacaa gccggccctc
     2941 ctgctgcggc gactgacggg gctcggcctc tgcccg
//
LOCUS       DASYEG010000035         8083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3781431, whole genome shotgun sequence.
ACCESSION   DASYEG010000035 DASYEG010000000
VERSION     DASYEG010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 8083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8083
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3781431"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="VGM72_01440"
     CDS             complement(<1..830)
                     /locus_tag="VGM72_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY4263954.1"
                     /translation="MKRWLKRIGLSLAGLAFIAGLLLVDFVRSFQRSVPSYDGTLNVT
                     AVAAPVQILRDRYAIPHIFAASLPDAAFGLGYVHAQDRLWQMETSRRFIQGRLAELFG
                     ASAVGTDTMMRAMDLYGAAQEAVKHLSPESRLVLQAYADGVNTYLKGHKGPLGIEFAL
                     AGDTPEPWVPADSIAVLKGMAMQLSGNAFSEAARASLIPVLGRMGVQDFFLPFGDAPL
                     PAYLDTLYGTTQTGQAYGIPDITASDNWAVDGAHSVTGKPLLANDPHLGFSIPSVWYL
                     A"
     gene            963..1421
                     /locus_tag="VGM72_01445"
     CDS             963..1421
                     /locus_tag="VGM72_01445"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY4263955.1"
                     /translation="MKTQSLDRDPIVVLSDVARLIRTRADARARAHGMTRAQWMILVR
                     LEWQPGMSQNELAALIEVEPITVGRLVDRLETRGFVERRPDASDRRIWRLHLMPAATP
                     MLEEIAKARAELNAMLVAAIPEQRLDGAIDCLLQMKTNLGADVRTSVKSA"
     gene            1428..2525
                     /locus_tag="VGM72_01450"
     CDS             1428..2525
                     /locus_tag="VGM72_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEY4263956.1"
                     /translation="MDAIVENRREALKAENPNRRRLVGRVLMLAGVAIVVVGGLAFWL
                     TGGRYVSTDDAFVDANKLMVSTDVSGLVLDIDVKEGQSVHKGDVLFRLDPKPFQIALD
                     QAQAKMAESALTIKSMEQDYKRMQSDIQAQRAQVALADTTLKRQNALLRIGGTAQQNV
                     DQAMATLNTSQSQTASLQQQAEVQLAKLGGRIGAPLESHPDYMQAKAARDEAQRQLDH
                     TVVKAPYDGTVTQVAALQPGAMIVSSLASFVPTSAVALVSDGAKWVTANMKETDLTYV
                     HPGQPVKMTIDTYPDRVWHGTVASIAPATGAQFSVLPSENSSGNWVKVVQRLQVKIAF
                     DAGEDISMVRAGMSTNVSIDTNHKRKLSDLF"
     gene            2525..4069
                     /locus_tag="VGM72_01455"
     CDS             2525..4069
                     /locus_tag="VGM72_01455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY4263957.1"
                     /translation="MADAVLGESMATRHRVLVVGCTMIATLMQALDNTIANVALPHMQ
                     GSLGASRDQITWVLTSYVIAAAIMTAPVGWLAARFGRKQFFIVCLVGFTVTSMACGLA
                     QNLPQEVVFRFLQGCFGAALVPLSQSIMLDLYPPQQRGMVMAVWATGVMIGPILGPTL
                     GGYLTDLYNWRWVFFINVPFGIVAVAGLVLFLGEQRRDSSLRFDWMGFAALSLGLGAM
                     QLAFDRGSSQDWFNSYEIIVETVLAGLGFYTFVIHMFTADKPFIPPRLFKDVGFLSGF
                     FTMFAVGVVLFGSSALLPPYLQNLGGYSVTQAGLLMTPRGIGTMVMMMVAGRLSSMID
                     PRFLIGVGVVFLAGAMWDMTLWTPDVSVWRLSVITMIQGAGIAFVFSPLQVVAFSSLA
                     PNLRTDAAAILSLSRNVGSAIGITATSVLLASNVQTLHAQYSEQITLFNRSLQSGATG
                     LFLGPTTPFGAQGIEAHIQYQALVASYANDFLLMFWVCVPLLPLIFFMKKPPKAQAAH
                     AEMAMD"
     gene            4163..4564
                     /locus_tag="VGM72_01460"
     CDS             4163..4564
                     /locus_tag="VGM72_01460"
                     /inference="COORDINATES: protein motif:HMM:NF022610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HEY4263958.1"
                     /translation="MISLPSHRVFRTLALWLGLAALMIQGLAPLCAGAMSGSGGASIV
                     ICTAHGSQTVQLDSDGKALPGGPAKLAADCCTVCHAPNGFTISSPILAALPSEAVHET
                     VRSEAAPLILPRSYSSYISRGPPPAVHYASA"
     gene            4625..6919
                     /locus_tag="VGM72_01465"
     CDS             4625..6919
                     /locus_tag="VGM72_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4263959.1"
                     /translation="MSCQATRAALVAGAGALLCAETVHAQASAPEQVVVEGARSVAER
                     DALPTTVESITFGELTSSVNLTNTEDALKYLPDILVRKRHIGDTQAPITTRTSGVGSS
                     ARSLIYADGVLLSALIGNNNSFASPRWGMIAPEEIDRIDVLYGPFAAQYPGNSIGAVI
                     EIATRMPDRYVATARVQTVVQDFSQYATHSGYPAYELSALIGDRSGKFSWRASINHLT
                     SRSQPLAYVTAARPSSPSAGGVAVTGAFDDVNRNGAAIAVLGAGGLEQQRQDNFTVKF
                     AYDLAPGVSIAYTAGLFLNDDKAGAQTYLKNASGVPVYSGNLNSAGFNITVPSSAFSN
                     NVYRLEETHWMHSVALNSGADGSLGWSVTGSLYDFNKDRQRFPAAALPAAFNGGAGTL
                     TDMGGTGWYTLDAKAFWKPDGEDGAHRLSFGAHDDRDTLSSDKYNIANWMTGSVGPLA
                     TAANGKTQTYAFWAQDVWRFAPGFTLTLGARYESWQAYGGFNASLSPPLSVAQPKLHS
                     SDVSPKASLGWQIAEDWFASASFGGAYRYPTVSELYQAVTTGAVLTVPNPDLKPEHAL
                     STELAVTRMTSEGKLRLSLFSEDISDALISQSAPLLPGSTTLFNYVQNIDHVRSYGAE
                     VSVERNDVLISGLTLQGSVTYVDPRIVSDPAFPAAAGKRIPQVPAWRGTLVATYRPDG
                     RWSYTLAARASTRVYAAADNSDTVTHTYQGFDSYFVIDARVNYQIDAHWNAAFGIDNL
                     NNRKYFLFHPFPQRSAIFELTYNY"
     gene            6945..7844
                     /locus_tag="VGM72_01470"
     CDS             6945..7844
                     /locus_tag="VGM72_01470"
                     /inference="COORDINATES: protein motif:HMM:NF019599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEY4263960.1"
                     /translation="MRIFLAVTALLPIFGIAFPASAHIVLSQPSFEAGQNYAAFFKVE
                     HGCDGSPTVALQVRIPDDVAVLDMPQKPGWTLNAERSKGRIASVTWRGKLEAKTSDQF
                     GLLLKLPANPGTLYFPVSQRCEKGETHWTDIPAAGQAWRDVPHPAPMLQLTAAVTPPS
                     YMAGSIMIEQPWSSATPNGAANGVAYMTVMNHGTAPDTLMGGSTPVAGKIEVHRMSSA
                     NGVMTMRLASGGIVIPPGGTVTLGPEANYHLMLTGLKAPLTQGSHVPATLDFAKAGSV
                     RVELAIAPIGARAPMAMPGMDHH"
     gene            7841..>8083
                     /locus_tag="VGM72_01475"
     CDS             7841..>8083
                     /locus_tag="VGM72_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY4263961.1"
                     /translation="MKAANSNRPYHHGDLAVSLREAARAILEEEGLAALSLRSVARRA
                     GVSHAAPYRHYASREALLADIAVEGLGRLRAELAQAA"
BASE COUNT         1465 a         2504 c         2485 g         1629 t
ORIGIN      
        1 gcgagatacc agaccgacgg aatggagaat cccagatgcg ggtcgttggc aagcagcggc
       61 ttgccggtga cggaatgagc gccatccact gcccaattgt cggacgccgt gatgtccgga
      121 atgccataag cctgcccggt ctgggtcgtg ccgtagagcg tatcgagata ggcgggcagg
      181 ggagcatcgc caaagggcag gaaaaaatcc tggacgccca tgcggcccag cacggggatg
      241 agagatgcgc gggccgcctc ggaaaacgca tttccggaaa gctgcatcgc catccctttg
      301 agcacggcga tgctgtccgc cgggacccag ggttcgggcg tgtcgcccgc gagcgcgaat
      361 tcgatgccga ggggaccctt atgccctttc agataggtat tgactccgtc ggcataggcc
      421 tggagcacaa gccgcgattc cggcgacaga tgtttcaccg cctcttgcgc ggcgccgtac
      481 aggtccatcg cccgcatcat ggtgtcggtg cccaccgcgg atgcgccaaa aagctccgcc
      541 agccggccct ggataaagcg tcgtgacgtt tccatctgcc acagccggtc ctgcgcatgg
      601 acgtagccga gcccgaacgc cgcgtcgggc aaggaagccg cgaagatatg gggaatggcg
      661 tagcggtcgc gcaggatctg aaccggcgcg gctactgcgg tgacgttcag ggtgccatca
      721 taggatggca cggaacgctg gaaggagcgg acaaaatcca ccagcagcag gccggcaata
      781 aaagccaatc ccgccaagct taggccgata cgcttcagcc agcgtttcac gcgttcctca
      841 tctcccgtcc ggatatggac ggttttcagg gccgccgggt caagattttc atgaaatttc
      901 tttgggagag cccatccggc cctcttgcgg aattaataac gtggcttata ttcacctacg
      961 ttatgaaaac gcaatccctc gatcgcgacc ccattgtcgt cctgtcggat gtcgcccggt
     1021 tgatccgcac gcgcgccgat gcccgcgccc gggcccatgg catgacccgc gcccagtgga
     1081 tgatcctggt tcgtctggaa tggcagccgg ggatgtcgca gaacgagctc gcggcattga
     1141 tcgaagtcga gccgatcacg gtcgggcgcc tggtggaccg gcttgaaaca cgaggcttcg
     1201 tcgaacgccg ccccgatgcc tcggaccggc gcatttggcg gcttcatctc atgccggcgg
     1261 cgacccccat gctggaggag atcgccaaag cgcgtgcgga gttgaacgcc atgcttgtgg
     1321 cggcgattcc cgagcaacgc ctggacggcg caatcgactg ccttcttcaa atgaaaacca
     1381 atctcggcgc cgacgtgcgc acgagcgtca agagtgcgta ggaccccatg gatgccattg
     1441 tcgaaaatcg ccgcgaggcg ctgaaagcgg aaaatcccaa tcgccgccgg cttgtcggcc
     1501 gcgtgctgat gctcgcgggc gtcgccatcg tcgtggtggg aggtttggcc ttttggctca
     1561 ccggcggacg ttacgtcagc acggacgatg cgtttgtcga cgccaacaag ctgatggtgt
     1621 ccaccgatgt ctcgggcctc gttttggata tcgatgtcaa ggaaggccaa agcgtccaca
     1681 agggcgacgt gctgttccgg cttgatccca agccgttcca gatcgcgctg gatcaggcgc
     1741 aagccaaaat ggcggagagc gcgctcacca tcaaatcgat ggagcaggat tataagcgca
     1801 tgcagagcga catccaggcg cagcgcgcgc aagtcgcgct cgccgacacc acgctgaagc
     1861 ggcagaacgc gcttttgcgc atcggcggca ccgcgcagca gaatgtcgat caggccatgg
     1921 cgacgctgaa tacgtcgcaa tcccagaccg catccttgca gcagcaggcc gaagtccagt
     1981 tggcgaagct cggtgggcgt atcggtgccc cgctcgaaag ccaccccgat tacatgcagg
     2041 ccaaagccgc gcgcgacgaa gcccagcgtc aattggacca tacggttgtg aaggcgccct
     2101 atgacggcac ggtgacccaa gtcgccgcct tgcagcccgg cgcgatgatc gtctcttccc
     2161 ttgcgtcgtt cgtaccgacg agcgcggtgg cgctggtgtc cgatggcgcc aaatgggtca
     2221 cggccaatat gaaggaaacc gaccttacct atgtccatcc cggacagccg gtgaagatga
     2281 ccatcgatac gtatcccgac cgcgtctggc acggcacggt tgcctcgatc gcaccggcga
     2341 cgggggcgca attctcggtg ttgccgtccg agaattccag cggcaattgg gtgaaggtcg
     2401 tgcagcgcct gcaggtcaag atcgctttcg atgcgggcga ggacatttcg atggtccgcg
     2461 ccggcatgag cacgaacgtc tccatcgaca ccaatcataa gcgcaagctc agcgacctct
     2521 tctgatggct gacgcggttc tcggcgaatc catggcgacg cgccaccgcg ttctggtggt
     2581 gggttgcacc atgatcgcaa cgctgatgca ggcgctggac aacaccatcg ccaatgtcgc
     2641 cctgccgcat atgcagggaa gcctcggggc cagccgggac cagatcacct gggtgctgac
     2701 atcctatgtg atcgcggcgg cgatcatgac cgcgcccgtg ggctggctcg cggcgcgctt
     2761 cggccgcaaa cagttcttca tcgtctgcct tgtcggattc acggtcacgt cgatggcgtg
     2821 cggtctggca cagaaccttc cccaggaagt ggtgttccgt ttcctgcagg gatgtttcgg
     2881 cgccgcgctc gtgccgcttt cgcaatccat catgctggac ctttatccgc cgcagcagcg
     2941 cggcatggtg atggcggtat gggctaccgg cgtgatgatc ggtcccatcc tgggcccaac
     3001 gcttggcggc tatctgaccg atctctacaa ttggcgctgg gtgtttttca tcaatgtgcc
     3061 gttcggcatc gtcgcggtcg cgggcctggt gttgttcctc ggtgagcagc gccgtgactc
     3121 gagcctgcgt ttcgattgga tgggcttcgc ggcgctttcg ctcggccttg gcgccatgca
     3181 attggcgttc gaccggggct catcccagga ttggttcaat tcctacgaga tcatcgtcga
     3241 gacggtgctc gcgggccttg gcttctacac cttcgtcatc cacatgttca cggccgacaa
     3301 accgttcatt ccgccgcgtc ttttcaagga tgtcggattt ctttcgggat tcttcaccat
     3361 gttcgcggtg ggcgtcgtgc tgttcggaag ttccgccctg ctgccgccct atctgcagaa
     3421 tctcggcggt tattcggtga cgcaggccgg tcttctgatg accccgcggg gcatcggaac
     3481 catggtcatg atgatggtgg cggggcggct gagcagcatg atcgacccgc gctttctcat
     3541 cggggtaggc gtggtcttcc tcgcgggcgc gatgtgggac atgaccctat ggacgcccga
     3601 tgtcagcgtg tggcggcttt ccgtcatcac gatgatccag ggtgcgggca ttgccttcgt
     3661 gttcagtcct ttgcaggtgg tggccttttc gtcgcttgcc ccaaatctca ggaccgatgc
     3721 cgccgcgatt ctttcgcttt cgcgtaatgt cggcagcgcc atcggcatca ccgcgacgtc
     3781 ggtgcttctc gcctccaacg tgcagacctt gcacgcgcaa tattccgagc agatcacgct
     3841 cttcaatcgt tcgctacagt cgggtgcgac cgggctattc ctcggaccga cgacgccgtt
     3901 cggagcccag gggatcgagg cgcatatcca ataccaggca ctcgttgcgt cctacgccaa
     3961 tgatttcttg ttgatgttct gggtctgcgt accgttgctg cccctgatct tcttcatgaa
     4021 gaagcctccc aaggcgcagg cggcccacgc cgaaatggcg atggactagg cggcaatggc
     4081 tcggacgatc cgccgcgttc cccgtagtcg atgcgagttg ctataaggga ttcgcatcct
     4141 ccggatcctt gatgtgaacc gcgtgatttc tttgccaagc caccgcgtct tcagaacgct
     4201 ggctctgtgg cttggcctcg ccgctttgat gattcaaggt ctggcgccgc tttgcgccgg
     4261 agcgatgagc gggagcggcg gcgcttccat cgtcatctgc accgcgcacg gttcccagac
     4321 ggttcaactg gacagcgatg gcaaggcgct gcccgggggg ccggcgaagc tcgcggcgga
     4381 ttgctgtacc gtatgtcacg caccgaacgg attcacgata tcatcgccaa tccttgcggc
     4441 acttcccagc gaagctgtcc atgagaccgt gcgttccgaa gcggcgcctc tcattctgcc
     4501 gcgttcctat tcctcctaca tcagccgagg gccgccgcct gcggtgcatt acgcatccgc
     4561 atagccggcg ctgcttcctc aattgagggg cgccgaggcg ttcctttgga agtaggagaa
     4621 tttcatgtct tgccaagcaa cccgcgcggc ccttgtggca ggcgccgggg cgctcctatg
     4681 cgccgagacc gttcatgcgc aagcaagcgc gcccgaacag gtcgtcgtgg aaggtgcgcg
     4741 gtccgtggcg gagcgcgacg ctctgcccac gacggttgaa agcattacct tcggcgagct
     4801 gaccagctcg gtgaatctca ccaacaccga agacgcgctg aaatatctgc cggatattct
     4861 cgtgcggaaa cgtcatatcg gcgacacgca ggcccccatc acaacccgaa cctccggcgt
     4921 gggatcgagc gcgcgcagcc tgatctatgc cgacggggtg ctgctgtccg cgcttatcgg
     4981 caacaacaat tctttcgcca gcccgcgatg gggcatgatc gcgcccgagg agatcgaccg
     5041 catcgatgtt ctctacggcc cttttgccgc gcaatatccc ggaaactcca tcggggcggt
     5101 gatcgagatt gcgacacgca tgcccgatcg ctatgtcgcg accgcgcgcg tgcagaccgt
     5161 ggtgcaggat ttcagccaat atgcgacgca ttccggctat ccggcctacg agctttccgc
     5221 tctcatcggc gaccgctccg gaaaattctc ttggcgcgcc agcatcaatc acctcaccag
     5281 ccgcagccag ccgctcgcct atgtcacagc ggcccggccg tcatcgccaa gtgctggcgg
     5341 tgttgcggtc accggcgcct tcgacgatgt gaaccgcaac ggcgccgcga ttgccgtcct
     5401 cggagccggg ggactcgaac agcagcggca ggacaatttc acggtgaaat tcgcctatga
     5461 tctcgccccc ggcgtcagca tcgcctacac ggctggactg ttcctcaatg acgacaaagc
     5521 cggtgcgcag acttatctga aaaacgcgtc gggcgtgccg gtctattccg gcaatctgaa
     5581 cagcgcgggt ttcaatatca cggtgccctc cagcgcgttc tcgaacaatg tctaccgttt
     5641 ggaggaaacc cattggatgc acagcgtggc gctcaactcc ggcgcggatg ggagcttggg
     5701 ttggagtgtg accggcagcc tgtatgactt caacaaggac cgccaacgct ttccggccgc
     5761 tgcattgcct gccgcgttca acggcggcgc gggcaccctg accgatatgg gcggcacggg
     5821 ctggtacacg ctcgatgcca aggcattctg gaagcccgac ggcgaggatg gggcccatcg
     5881 cttgagcttt ggcgcgcatg acgatcgcga cacgctttcg agtgacaaat acaacatagc
     5941 gaattggatg accggttcgg tgggaccgct tgccaccgcg gccaatggca agacccagac
     6001 ctatgccttt tgggcgcagg acgtctggcg ttttgcgcct ggcttcactt tgactctcgg
     6061 tgcgcgctac gaatcctggc aggcctatgg cggcttcaac gcttcgctgt cgccgccgct
     6121 ttccgtggcg cagccgaaac tgcattcgtc cgatgtctct cccaaggcct cacttggctg
     6181 gcagattgcc gaagattggt tcgcttcggc ctcgttcggc ggcgcctatc gctatccgac
     6241 ggtcagcgag ctttaccaag ccgtcacgac cggcgcggtg ctcacggttc ccaatcccga
     6301 tctgaaaccg gagcatgctc tttcgacgga gcttgccgtg acgcgcatga cgagcgaggg
     6361 gaagctgcgc ctatcgctgt tctccgaaga catttcggat gcgctgattt ctcagagtgc
     6421 acccttgctg cccggttcga cgacgctgtt caattatgtg cagaatatcg atcatgtgcg
     6481 cagttacggc gccgaagtct cggtcgaacg gaacgacgtg ctcatcagcg gcctcacgct
     6541 gcaggggagc gtgacctatg tcgatccacg cattgtttcc gaccccgcct ttccggcagc
     6601 cgccggaaag cgcattccac aggttcccgc ttggcgcgga actctggttg cgacctatcg
     6661 gccggacggc agatggagct atacgctggc cgcgcgcgcc agtacgcggg tctatgcggc
     6721 cgccgataat tccgacactg tgacgcacac ctatcagggg ttcgatagct attttgtgat
     6781 cgatgcccgg gttaattatc agatcgatgc ccactggaat gccgcttttg gcatcgacaa
     6841 tctcaacaat cggaaatatt tcctgttcca cccattccca cagcgttcgg cgattttcga
     6901 attgacgtac aattactgag gcgttgcgtg aaaggagaaa acacatgagg atatttctcg
     6961 ctgtcacggc gcttctgccg attttcggca ttgcgtttcc ggcatccgcg cacatcgtgc
     7021 tctcgcagcc gagtttcgaa gccggacaga attacgctgc cttcttcaag gtcgaacatg
     7081 gctgcgacgg ctctcccacc gtggcgcttc aggtgcggat tcccgatgat gtcgccgttc
     7141 tggatatgcc gcagaagcct ggctggacat tgaacgctga gcgtagcaag ggtcgcattg
     7201 ccagcgtgac gtggcgcggg aaactggagg cgaagacgtc cgatcagttc ggtcttttgc
     7261 tgaagctgcc ggcgaacccc ggcacgctct atttcccggt gtcgcagcga tgcgaaaagg
     7321 gcgagacgca ttggaccgac attcccgccg ccggtcaggc ttggcgggat gttccgcatc
     7381 ccgcgccgat gctgcaattg accgcggcgg tgacgcctcc gagctatatg gcaggcagca
     7441 tcatgatcga acagccctgg agttcggcga cacccaatgg cgcggcgaac ggcgtggcct
     7501 acatgaccgt catgaatcac ggcacggcgc cggatacgct catgggcggc tccaccccgg
     7561 tggccgggaa aatcgaagtg catcggatgt cgtcggcgaa cggtgtgatg acgatgcgtc
     7621 tggcgtcggg aggcatcgtc attcctcctg gcggcacggt cacgctgggc ccggaggcga
     7681 actatcattt gatgctgacc ggactgaaag cgccgctcac ccaaggttcg catgtccccg
     7741 cgacgctgga tttcgccaag gcagggtccg tgcgcgtcga acttgcgatc gctcctatcg
     7801 gcgcccgtgc tccgatggcc atgcccggga tggatcatca ttgaaggcgg ccaattccaa
     7861 ccgtccctat catcatggcg atctcgcggt cagcttgcgc gaagccgcgc gcgcgatcct
     7921 ggaagaagaa ggtttggccg cgctgtcgct gcgttcggtt gcgcgccgcg cgggcgtttc
     7981 ccatgcagca ccctatcgcc attatgcgag ccgcgaggcg ctgcttgccg acatcgcggt
     8041 ggaggggctg ggacgtttgc gtgccgagtt ggcgcaagcc gcc
//
LOCUS       DASYEG010000036         2032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_5085608, whole genome shotgun sequence.
ACCESSION   DASYEG010000036 DASYEG010000000
VERSION     DASYEG010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5085608"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..1772
                     /locus_tag="VGM72_01480"
     CDS             54..1772
                     /locus_tag="VGM72_01480"
                     /inference="COORDINATES: protein motif:HMM:NF006203.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEY4263962.1"
                     /translation="MPTKTIASGTVADAYLELLADRGIDYLFANAGTDFAPLIEAYAK
                     AQAHGTKVPKPITVPHENVAIAMAQGYYLKTGRPQAVMVHVNVGTANAICGLINGWRG
                     NIPVLFTAGRTPFSEEGGLMGMRSGEIHWPQEMRDQRAMVREFVKWDYELPHGEVLEA
                     AVDRALNIAMSEPMGPVYMTLPRETLAAPIRDFRYSSPSRHATPSPAFPDTRAIDEAA
                     GMLAAAEFPLIITRTAGRIEADVAKLAGFAERFAIPVFERKHHYMCMPSDSAMNLGGN
                     PESYFDIADVIVVLEADVPWIPKHKAPREGTKIIHIGADPIFANYPLRGFPCDLAITG
                     VLGAVLPALSEALALREKNAAARIASRRRTLAQARETIAARTAASLERVRNGSPIHPG
                     WINHCIDRVKGADGIVIKEALTPAEHLNFTKPGTYYSLGQGGALGWGLGTALGMKAGA
                     PDRLVICAVGDGSYMFGNPLAAHYVGKAEKLPTLTVIYNNEMWNAVRRNTRDVYPDGY
                     AARSNREPLTYFEPGTRYEKAIETVGGYGERVEDAAALPKAFDRALDAVASGQQALLN
                     VICGTG"
BASE COUNT          369 a          649 c          649 g          365 t
ORIGIN      
        1 atgacttcat ccggcttgcc gttccgttcc tgaaatctta atcggagttc ttcgtgccga
       61 ccaaaaccat cgcgtccggc actgtcgccg atgcctatct ggaactcctc gccgatcgcg
      121 gcatcgatta tctcttcgcc aatgcgggca ccgatttcgc gccgctgatc gaggcgtacg
      181 ccaaggcgca ggcgcacggc accaaggtgc ccaagccgat cacggtgccc catgagaatg
      241 tcgccatcgc catggcgcaa ggctattatc tgaagaccgg ccggccgcag gcggtgatgg
      301 tccatgtcaa tgtcggcacc gccaatgcga tctgcggcct gatcaatggc tggcgcggca
      361 atattccggt gctgttcacc gcgggtcgca cgccgttctc ggaagagggc gggctgatgg
      421 ggatgcgcag cggcgaaatc cattggccgc aggagatgcg cgatcagcgc gcgatggtgc
      481 gcgaattcgt caaatgggat tatgagcttc cccatggcga ggtgctggaa gccgccgtcg
      541 accgcgcgct gaacatcgcg atgagcgagc cgatgggccc ggtctacatg acgctgccgc
      601 gcgagacgct ggcggccccc atcagggatt tccgctattc ctcgccgtcg cgccatgcca
      661 cgccgtcacc ggcttttccc gacacccgcg ccatcgatga ggccgccggg atgctggcgg
      721 cggcggaatt tccgctcatt atcacccgca ccgcggggcg catcgaagcc gatgtggcca
      781 agctcgcagg cttcgcggag cgtttcgcca ttccggtgtt cgagcgcaag catcattaca
      841 tgtgcatgcc gtccgacagc gcgatgaatc tcggcggcaa tccggaaagc tatttcgaca
      901 tcgccgatgt catcgtggtc ctggaagccg atgtgccctg gatccccaag cacaaggcgc
      961 cccgggaggg caccaagatc atccatatcg gtgccgaccc gatcttcgcg aattatccgt
     1021 tgcgcggctt tccctgcgat ctggcgatca ccggcgtgct cggcgcggtc ttgccggcat
     1081 tgagcgaggc gcttgccctg cgcgagaaaa atgcggccgc gcgcatcgcg tcacggcgca
     1141 ggacattggc gcaagcccgc gagacgatag cggcgcgcac cgcggccagt ctggaacggg
     1201 tgagaaacgg atcgcccatt catcccggct ggatcaatca ttgcatcgac cgggtcaagg
     1261 gtgcggacgg catcgtcatc aaggaagcgc tgacgcccgc cgagcatctg aacttcacca
     1321 agcccggcac ctattattcg ctggggcagg gcggcgcgct gggctggggt ctgggcacag
     1381 cgctcggcat gaaggcgggt gcgcccgaca ggctcgtcat ctgcgcggtg ggcgatggct
     1441 cctatatgtt cggcaatccg ctggccgccc attatgtcgg caaggccgag aagcttccga
     1501 ccctgacggt gatctataac aacgagatgt ggaacgcggt caggcgcaac acccgcgacg
     1561 tctacccgga cggctatgcc gcccggagca atcgcgagcc gctgacctat ttcgagcccg
     1621 gcacgcgcta tgagaaggca atcgagacgg tgggtggtta tggcgagcgg gtggaggatg
     1681 ccgccgccct ccccaaggcg tttgaccgcg cgctcgatgc cgtggcgagc ggccagcagg
     1741 cgctcctcaa cgtcatctgc ggcacaggat aattaatccg tatacggaat aattatgtag
     1801 gcgcgcacag tcgcgcgaaa aacgagggga tggcgtgagg cgccgtgtcc ggcgatgacc
     1861 acacgtgttt aaagaaggtg ccgtcttgcg gccctcacgc ccggcgattt tgggcgcttc
     1921 gctttccgat ttcgccgcaa aggctcgaag cctgccggct catcggcaac ccgtcctccc
     1981 gcccgccctt cctgtcgaaa gggtagcggg tcggccggaa agcgccgcct tc
//
LOCUS       DASYEG010000037         6486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4070951, whole genome shotgun sequence.
ACCESSION   DASYEG010000037 DASYEG010000000
VERSION     DASYEG010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6486
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4070951"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VGM72_01485"
     CDS             complement(<1..186)
                     /locus_tag="VGM72_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263963.1"
                     /translation="MRSFRSLISATGLACLTLLSSSARAAGVADFYKGRTVQVLIGFS
                     AGGGYDIYARTLARYMGK"
     gene            complement(313..2331)
                     /locus_tag="VGM72_01490"
     CDS             complement(313..2331)
                     /locus_tag="VGM72_01490"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY4263964.1"
                     /translation="MPGQWEMALHALQLLLEPARLIILVLGVLIGLGIGCMPGLGGVV
                     ALAVLIPFTYNLDAPTSFALLLGVAAVTTTSDLIPAVLFGVPGTVGAAATVIDGHQMA
                     RQGKAGRAFGAGFVAAAIGGIFGAFVLALAIPLLQPVMLAVGSPELLAFSIFGLSMVA
                     TLSGRMPLKGLTAAGIGLMISMIGSGTQTGTLRWTFDWLYLFDGVPLIPVTLGLFALP
                     ELADLAINRKRIAGERLDVTVSSQWEGVRDVFHNWKLVLKCSALGTALGIVPGIGSAV
                     IDWIVYGYAVRTEKNPETFGTGDVRGVIAPESSNNAKEGGHLVPTIAFGVPAGASMTL
                     LLGAFLLHGLTPGPDMLTKNLDITYSIIWSLTIAHVLGTTICLLGARQLARFAEMRHE
                     ILLPMVMPIVLVATFQATRSWGDLYFLLIFGTLGWIMKRFGWPRPPMVLGFVLGPIFE
                     RYLFLSNEIYGARWMARPVVIAIAILIAWALYRPLRQTVARLVHEFRHLSRAHLRLKP
                     RAMFTLVLIAVIVAALVTSASWPPIEQIVPRTACWAALIVASLNLVTEIFGADEATAE
                     PPAAHGAEPEALIPAGIAVKRAAAFLAWLIAMVALAATIGFIPAIGVFVFVYMGIGFR
                     EPIWRAALFGTAMTLFCYAVFDRGLSVPWPQAVLGDWVPALREASGLM"
     gene            complement(2452..2997)
                     /locus_tag="VGM72_01495"
     CDS             complement(2452..2997)
                     /locus_tag="VGM72_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY4263965.1"
                     /translation="MKNLDERDFAHTGDKTCTFRTDDIKNSADFRAAYEHAFSVISAE
                     DQPFEHSPDGLIKHLVHEKMGTREQCVDAYMLFLQEQGQSGKHRHMWEEVIFVAEGSG
                     YDLHWDVKFDCIDTYHWEWEETPRKSQWERGDFIYVPPYSIHQHFNTGKGEVRLIVIS
                     NRIVKAMGFNWLDQVENAPGA"
     gene            complement(3068..3670)
                     /locus_tag="VGM72_01500"
     CDS             complement(3068..3670)
                     /locus_tag="VGM72_01500"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY4263966.1"
                     /translation="MSAPPAGAPQAQEASLPGPKHNQEAPVVWERWQKKRTFVRALEG
                     TYSHLTKELLDAPRVISSKSVPWKGGPNLYGKHPIAPGGVAKITQAIECHVEAYGPGA
                     YSQKHGHLNSACFYIIKGRGHDIHDGKHIPWKAGDVMFVENGCVHQHFNDDPDNEALM
                     IVFKAKPLFLFMHLLYQKMISYPPSEPTEGHKGWTPPTDF"
     gene            3977..4753
                     /locus_tag="VGM72_01505"
     CDS             3977..4753
                     /locus_tag="VGM72_01505"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEY4263967.1"
                     /translation="MARTISNVWVNSEYKHLIVEASPKQLRCAPGQFFNILCPSPDDG
                     DLWLRRPQSIYRIDREEGHLEFLYKCVGRGTRGMATFKPGDPCNMVGPLGVGFSLRPD
                     WRNIVVLGRGVGLATLGPISQLAAENNVGVTAILSARSPDLVMADELFGKSGEVVRVL
                     DSDGTSAVENVEAIIERRIADGKADAFYTCGSNRLLFLMKAIGRKHNIPGQVAMEQIM
                     ACGLGPCYVCVRTFEVDGHKELRRVCIEGPVFDLQECVGW"
     gene            4747..5733
                     /locus_tag="VGM72_01510"
     CDS             4747..5733
                     /locus_tag="VGM72_01510"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEY4263968.1"
                     /translation="MVDLSVRIGSVSLKNPIMPASGCFSTDMAQIMDVNRLGALVAKT
                     VSREYRIGNPPPRVAELECGMINSIGLPTKGIGYFLEHQVPDYARFTPPLVGSISATT
                     AEDFEAMTREVGGHPDVDVVEVNISCPTRNPGGGNFAMHEDHTYKVTKQIRDATGKPV
                     WVKLSPNAGDVVAIGQAAEAAGADALVVSNTLLGLKINIDTFRPAIGNKFGGISGPGV
                     KPIIMRMVHQVSQKVKIPVIGCGGIVKVEDVVEYMLAGASAVMLGYIVFRNPSALVGM
                     IDELEAWCEKRGFARAADLTGAMIDEPIAETFAAAAAPIGQGHQTFVPQAAE"
     gene            5740..>6486
                     /gene="pyrC"
                     /locus_tag="VGM72_01515"
     CDS             5740..>6486
                     /gene="pyrC"
                     /locus_tag="VGM72_01515"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY4263969.1"
                     /translation="MAERITLRKPDDWHLHVRDGAMLKAALPFTAAHFGRAIIMPNLL
                     PPVLNVKDAIAYRDRVMAALPEGAKFQPLMTCYLTDATDADDVETGFREGVFTAVKMY
                     PANATTNSAYGVTDFQRIRAVLARMEKIGMTFLIHGEEADPEIDVFDREVVFIERRLA
                     PMAKDFPGLRIVLEHVSSREGVAFVRAHEGQIGGTITPYHLMLTRTDWLGYGNRPYMY
                     CMPVIKRREDRDALRKAAISGDSNFFLGTDS"
BASE COUNT         1275 a         1943 c         2045 g         1223 t
ORIGIN      
        1 ttttcccatg tagcgcgcca gtgtgcgggc atagatgtcg tagccgccgc ccgcggagaa
       61 tccgatcagc acttgaacgg ttctgccctt gtagaaatcc gcgacgccgg ccgcccgcgc
      121 cgaagaggag agcagggtca gacaggccaa tcccgtggcg gatatcagcg agcggaacga
      181 acgcatgcgc gaatcctctt ttgcgcgatt ttccacgaac caagaaaaac acaaagggca
      241 ccaagcttcc accatgttca ccaaggacat tttgcaccct tcgtgcgtcc ttatctggta
      301 tctcacctct ttctacatca agccgctggc ctcgcgcagc gcgggcaccc aatcgcccag
      361 cacggcttgc ggccagggga ccgacaatcc gcggtcgaac acggcatagc agaacagcgt
      421 catggcagtg ccaaacaacg cagcacgcca gatcggttcg cgaaagccga tccccatata
      481 gacgaagacg aatacgccga tggccgggat gaacccgatg gtcgcggcca atgccaccat
      541 tgcgatcagc caagcaagaa aggcggccgc gcgcttgacc gcgattcccg ccggaatcag
      601 agcttccggt tcggcgccgt gtgcagccgg aggctctgct gtggcctcgt cggcgccaaa
      661 gatctcggtc acaagattga gagatgcgac gatcaaggcc gcccagcatg cggtgcgggg
      721 cacgatctgt tcgatcggcg gccagcttgc cgaagtgacg agcgcagcca cgatcaccgc
      781 gatcaggacc agcgtgaaca tcgcgcgcgg cttcaacctc agatgcgcgc gcgacaaatg
      841 gcggaactcg tggaccagcc gcgcgacggt ctggcgcaga ggccggtaca gtgcccaggc
      901 gatcagaatc gcgatagcga tcaccaccgg ccgcgccatc cagcgtgcgc cgtagatttc
      961 gttggagagg aacagatagc gttcgaagat cggaccgagc acgaagccca gcaccatggg
     1021 cgggcgcggc cagccgaaac gtttcatgat ccagccaagc gtgccgaaga tgagcaggaa
     1081 atagagatcg ccccagcttc gcgttgcctg aaaggtcgcg accagcacga tgggcatcac
     1141 catgggcagc aggatttcgt gccgcatttc cgcaaagcgc gcgagctgtc gcgcacccaa
     1201 aaggcagatc gtcgtgccca gcacatgggc gatggtgagg ctccagatga tggaataggt
     1261 gatgtcgaga ttcttggtca gcatgtccgg gcccggcgtc agcccgtgca gcagaaacgc
     1321 tcccagcagc agcgtcatgg acgcacccgc cggcacgccg aaggcgatcg tgggaacgag
     1381 atggccgcct tccttggcat tgttcgagga ttccggtgca atgacgccgc gcacgtcgcc
     1441 ggtgccgaag gtttccgggt tcttctcggt gcgcacggca tagccgtaga cgatccagtc
     1501 gatgaccgct gaaccgatgc ccggcacgat gccgagcgcg gtgccgagcg cgctgcattt
     1561 cagcaccagt ttccaattgt ggaagacgtc gcgcacacct tcccattggc tcgaaacggt
     1621 cacgtccaga cgctcgccgg cgatgcgctt gcgattgatc gcgaggtcgg caagctccgg
     1681 cagagcgaac aggccgagcg tcacgggaat cagcggcacg ccatcgaaca gatagagcca
     1741 gtcgaaggtc catcgcagcg tgccggtctg cgtgcctgag ccgatcatgg agatcatcag
     1801 cccgatccct gcggcggtga gacccttgag cggcatgcgg cccgaaagtg tcgccaccat
     1861 ggaaagccca aaaatgctga acgcgagcag ctccggcgag cccacggcca gcatgaccgg
     1921 ctgaagcagg ggaatcgcga gcgccagcac gaacgcgccg aaaatcccgc ctatcgcggc
     1981 ggccacgaac ccggcgccga aggcgcggcc cgctttgccc tggcgcgcca tctgatggcc
     2041 gtcaatgacg gtcgccgcgg cgcccaccgt gccgggcacg ccgaacagca cggcgggaat
     2101 cagatccgat gtcgtggtga cggcggcgac gcccagcagc agcgcaaagg atgtcggcgc
     2161 gtcgaggttg taggtgaagg gaatcaacac ggcgagcgcg accacgccgc ccagccccgg
     2221 catgcagccg atgccaagcc cgataagcac gcccagtacg aggatgatca gccgggccgg
     2281 ttccagcagc aattgcagcg cgtgaagcgc catctcccat tgccccggca tcggctgcac
     2341 gattcccctg cttgcgctgc ggcgccaatg ccgcagcggc ggcggcttat cttattacgg
     2401 tgtgctcgtg cctcgcccgc tacacagacg cgtgatgaaa tcgcatctcg gtcatgcgcc
     2461 gggtgcgttc tccacctggt cgagccagtt gaaccccatg gccttgacga tgcggttgct
     2521 gatgacgatc agccgcactt cgcctttgcc ggtattgaaa tgctggtgga tggagtaagg
     2581 cggaacgtag atgaaatcgc cgcgctccca ttgggatttg cgtggggtct cttcccattc
     2641 ccaatgatag gtgtcgatgc agtcgaactt cacgtcccaa tgcaggtcgt agccggagcc
     2701 ttcggccacg aagatcacct cttcccacat gtggcggtgc ttgcccgatt ggccctgttc
     2761 ctgaaggaac agcatatagg catcgacgca ttgctcgcgg gtgcccatct tctcatggac
     2821 gagatgcttg atgagcccgt cgggggaatg ctcgaagggc tgatcttcgg cgctgatcac
     2881 gctgaatgcg tgttcatagg ccgcgcgaaa atcggcggag ttcttgatgt cgtcggtacg
     2941 gaaggtgcaa gtcttatcgc cggtgtgggc aaagtcgcgt tcgtctagat ttttcattcg
     3001 tctctccgga aaagcgaaaa tttggaaatc ggccgattgg aaatcggcca attaaaaaat
     3061 cagccaatta aaaatcagtc gggggcgtcc aacccttatg gccttcggtc ggttcgctcg
     3121 gcggatagga aatcatcttc tgatagagca agtgcatgaa gaggaagagc ggcttggcct
     3181 tgaagacgat catcaacgct tcattgtccg gatcgtcgtt gaagtgctga tgcacgcagc
     3241 cattctccac gaacatcaca tcgccggctt tccagggaat gtgcttgccg tcatggatgt
     3301 cgtggccgcg tcccttgatg atatagaagc aggccgaatt cagatgcccg tgcttctggc
     3361 tataggcgcc cggcccgtag gcctcgacat ggcattcgat ggcctgcgtg atcttggcca
     3421 cgccgcccgg tgcgatgggg tgcttgccgt aaagattggg gccacccttc caggggacgc
     3481 ttttcgatga gatgacgcgc ggtgcatcca gcagctcctt ggtcagatgg ctataggtgc
     3541 cttccagcgc gcggacgaag gtacgcttct tctgccagcg ctcccagacg acgggggctt
     3601 cttgattgtg tttggggccg gggaggctgg cctcttgcgc ctggggcgcg ccggcgggag
     3661 gtgcggacat gagggtttcc tttggttcgc gatgatggtg gggatgtttt cgaaccctct
     3721 ccggccttcg tcaagcagga gctggaaaga gggatgcgtc ctttggctcg tttggcgata
     3781 cgttttttcc attgtgagca cactgtacca aaaaaaggaa atatgttgat ttttgtacag
     3841 ccgaccgctt gaatagcgcg atccagatgt acgggcgcgc cgcgaaatga caaaaaccgc
     3901 ctttttatca aagccttatg ttgtcggcgg cgaaagcggc ggcgaatccg ctccgcacat
     3961 tccagccgag gaggcgttgg ccaggaccat ctctaatgtc tgggtcaatt ccgaatacaa
     4021 gcatttgatc gtggaagctt cgcccaagca gctccgctgc gctccgggcc agttctttaa
     4081 cattctttgt ccgtcgccgg acgatggcga tctctggctg cgccggcccc agagcatcta
     4141 ccgcatcgac cgggaagaag ggcacctcga atttctctat aaatgcgtcg ggcgtggcac
     4201 gcgcggcatg gcgaccttca agccgggcga tccctgcaac atggtgggcc cgctcggggt
     4261 cgggttttcc ctccggccgg actggcggaa tatcgtggtg ctgggccgcg gcgtggggct
     4321 tgccacgctc gggcccatct cccagctcgc cgccgaaaac aatgttggcg tgacggcgat
     4381 tttgtcggcg cgctcccccg atctggtgat ggccgatgag ctgttcggga agtcgggcga
     4441 ggtggtgcgc gtgctggaca gcgacggcac cagtgcggtt gagaatgtag aagccattat
     4501 cgaacgtcgg attgccgacg gtaaagcgga cgccttctac acctgcggct ccaaccggct
     4561 gctgttcctg atgaaggcga ttggccgaaa acacaatatt cccggccagg tggcgatgga
     4621 gcagatcatg gcctgcgggc ttggtccctg ctacgtctgc gtgcgcacgt tcgaggtcga
     4681 tggccacaag gaactgcgcc gggtgtgcat cgaggggccg gtgttcgatt tgcaggagtg
     4741 cgtaggatgg tagatctctc ggtcagaatc ggcagcgtgt cgctgaagaa cccgatcatg
     4801 ccggcctcgg gctgtttctc gaccgacatg gcgcagatca tggacgtgaa ccggctgggc
     4861 gcattggtgg ccaaaaccgt gtcgcgcgaa tatcgtatcg gcaatccgcc gccgcgcgtc
     4921 gccgagctcg aatgcggcat gatcaattcc atcggcctgc cgaccaaggg tatcggttat
     4981 ttcctggaac atcaggtgcc ggattatgcg cgcttcaccc cgccgctggt gggctcgatc
     5041 tcggccacga cggcggagga tttcgaggcc atgacccgcg aagtgggcgg tcatcccgat
     5101 gtcgatgtcg tggaggtgaa tatttcctgc ccgacccgca atccgggcgg cggcaatttc
     5161 gcgatgcacg aggatcacac ctacaaggtc accaagcaaa tccgcgatgc caccggcaaa
     5221 cctgtctggg tgaaattgtc gcccaatgcg ggcgatgtcg tggccatcgg ccaggccgcg
     5281 gaagcggccg gcgccgatgc gctggtggtc tccaacacgc ttctggggtt gaagatcaat
     5341 atcgacacgt tccgcccggc catcggcaac aaatttggcg gcatttcggg acctggcgtg
     5401 aagccgatca tcatgcgcat ggtgcatcag gtctcacaga aggtgaaaat cccggtcatc
     5461 ggctgtggcg gcatcgtcaa ggtcgaggac gtcgtcgaat atatgctggc gggcgcttcg
     5521 gcggtgatgc tgggctacat cgtcttccgc aatccgtccg cgcttgtcgg catgatcgat
     5581 gaattggaag cctggtgcga gaaacgcggg ttcgcgcgcg ccgccgatct gaccggcgcg
     5641 atgatcgacg agccaatcgc ggagacgttt gccgccgcgg ccgctcccat cggccagggc
     5701 caccagacct tcgtgccgca agccgccgag taggggcgca tggccgagcg catcaccctt
     5761 cgcaagccgg acgactggca tctgcatgtg cgcgacggag ccatgctcaa ggccgcgctg
     5821 ccgttcaccg ctgcccattt cggccgcgcc atcatcatgc ccaatctgct gccgccggtg
     5881 ctgaacgtca aagacgccat cgcctatcgg gaccgggtga tggcggcgct gccggaaggc
     5941 gcgaagttcc agccgctgat gacgtgctat ctcaccgatg ccacggacgc ggacgatgtg
     6001 gagacgggtt ttcgcgaagg cgtgttcacg gcggtgaaga tgtatcccgc caatgcgacg
     6061 acgaattcgg cctatggcgt caccgatttt cagcgcatcc gtgccgtgct cgcgcggatg
     6121 gaaaagatcg gcatgacctt cctcatccat ggcgaggaag ccgatcccga gatcgatgtg
     6181 ttcgaccgcg aggtggtgtt tatcgaacgc cggctcgccc ccatggccaa ggattttccc
     6241 ggccttcgca ttgtgctgga acatgtctcc tcgcgcgaag gcgtggcctt cgtgcgcgcg
     6301 cacgaaggcc agatcggcgg cacgatcacg ccctatcatc tgatgctgac acgcaccgat
     6361 tggctgggtt atggcaaccg gccctatatg tattgcatgc cggtgatcaa gcggcgggag
     6421 gatcgcgacg cgctccgcaa agccgcgata tcgggggaca gcaatttctt cctcggcacg
     6481 gattcc
//
LOCUS       DASYEG010000038         3681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1171848, whole genome shotgun sequence.
ACCESSION   DASYEG010000038 DASYEG010000000
VERSION     DASYEG010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171848"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VGM72_01520"
     CDS             complement(<1..620)
                     /locus_tag="VGM72_01520"
                     /EC_number="4.1.2.61"
                     /inference="COORDINATES: protein motif:HMM:NF006588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxycinnamoyl CoA hydratase/lyase"
                     /protein_id="HEY4263970.1"
                     /translation="MANDGMTRFNSIRIERDSGVAFVILNRPEKRNAMSPELHGEMCE
                     ALEELETEEETKIVVVTGAGEAFSAGQDIKLYFRGTEDNPKARAQARHDSHHWRWEKL
                     SKYAKPTIAMVNGYCFGGAFTPLCACDFAIAADEATFGLSEVNWAILPGGIVSWNVAQ
                     LMSYRDAMYYACTGETFDGKQAAAMKLVNFSVPRSELRAATLKFAAG"
     gene            749..949
                     /locus_tag="VGM72_01525"
     CDS             749..949
                     /locus_tag="VGM72_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263971.1"
                     /translation="MAGDSYITNPSAQQGESTLRKGSRGPANIGGNVQPEIQPEESAE
                     EGPVDIPEHAGKRPAPTLDRLR"
     gene            1087..1284
                     /locus_tag="VGM72_01530"
     CDS             1087..1284
                     /locus_tag="VGM72_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263972.1"
                     /translation="MDEDSAGRAKRYREHAAEIYAAAVGVTHPPSRAALLRLAETYDR
                     LAAKIDAEPRAGRAGKGLLDE"
     gene            complement(1297..>3681)
                     /locus_tag="VGM72_01535"
     CDS             complement(1297..>3681)
                     /locus_tag="VGM72_01535"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY4263973.1"
                     /translation="ESRARDLLSAKLYDGEEGRIGQRFRRLEDLPLLLGRGRFVDDIR
                     IPGILHVAFVRSQHAHATILSIDAEAARSAPGIHAVLTLDDLMPSLRIRRMVRQPAQG
                     TPRAELWPFALSPGEVAFVGEPIALVVAENRYLAEDAAALVDVTYDILPPVTDPREAA
                     VAGSPRVRSELASNIVATYKVTYGDTDAAFRNAAHVFKEELWQHRGSAHPIEPRGLAA
                     QYEPANDGVTVFASTQKAHDLYQNLSAFLALDENRLRVATPDVGGGFGPKLCVYPEDV
                     AVTAAAHLLKRSLKWIEDRREHFVGAVHERDQYWTIEIAVDDKGHVLGIRGRLFHDQG
                     AYAFQDVNLPYNSASGVSGPYKVPALHMDVVVAHTNKVPVSSVRGAGYPQAAFAMERL
                     LDRVAHELDIDRAELRRRNLIPPEKLPYEKPIKSRAGASIVYDSGDYPACQAELLQQA
                     GWESFPQRQKLAREEGRYIGIGLAHGLKGTGRGPFESAVVRVTGTGVVSVLTGAAAIG
                     QGLVTALSQICAEQLGMRPQDVHVIHGDTAVVPWGLGAFASRQTVTAGSSVYLAARAV
                     AEKARKLGGMMLQMPPDDLEVADGTVRAKADPSRAIALAELARILRGGPGYAFPPGFE
                     PGLEATACFRTDLLAYANVCHVAEVEVDIETAHVRVTRYLALHDSGVRINPMIVDGQT
                     HGSISHGLGNALYEWMGYDSHGQPTTTSFADYLIPTAPEVPRFETSYRQSPSPMNPLG
                     VKGAGEAGVIPVSPAVMSAIENALSPFGLRIAETPLLPARLHALIADARARGRR"
BASE COUNT          688 a         1153 c         1156 g          684 t
ORIGIN      
        1 ccagccgcga atttcagcgt ggccgcgcgc agttcgctcc tgggcacgga gaaattcacc
       61 agcttcatcg cggccgcctg tttgccgtcg aaggtctcgc cggtgcaggc atagtacatc
      121 gcgtcgcgat agctcatgag ctgcgccacg ttccagctca cgatgccgcc cggcaggatc
      181 gcccagttga cctcggaaag cccgaaggtc gcttcgtccg ccgcgatggc gaaatcgcag
      241 gcacacagcg gcgtgaaggc gcccccgaag caatagccgt tgaccatggc aatcgtgggc
      301 ttggcgtatt tcgacaattt ttcccagcgc caatgatgcg aatcgtggcg ggcttgcgcg
      361 cgcgctttcg ggttgtcctc ggtgccgcgg aaataaagct tgatgtcctg gcccgcggaa
      421 aaagcttcgc ctgcgccggt gacgacgacg atcttggtct cttcctcggt ttccagttcc
      481 tccagcgcct cacacatttc gccatgaagt tccggactca tcgcattgcg tttttcgggg
      541 cggttgagga tcacgaatgc gacaccgctg tcgcgctcga ttctgatgct gttgaaccgg
      601 gtcatgccat cgttcgccac gcaaacctcc cgaattgtca tagttttccg aagtcagcct
      661 ggcgtgggct tgccgccacc gacaagaccg agcctggaac aaaaaccatc catcggcgat
      721 tacccttcta tcagtaaagg agagttccat ggccggcgat tcctatatca ccaatccctc
      781 tgcccagcag ggcgaatcca cattgcggaa aggctctcgc ggtcccgcca atatcggcgg
      841 gaacgtgcag ccggaaattc aaccggaaga atccgctgaa gaaggcccgg tggatattcc
      901 cgaacatgcg ggaaaacggc ccgcgcccac tctcgataga cttcgctaaa cgggacgccg
      961 ctttcgcgga cttgcctctg tgaaagagca gcagcatatt atcgcgctgt cgtcgcgatg
     1021 acggcgaaga tgttgcctgc tgcttggggg gcgggcgggc tcccgggggc taacgggagc
     1081 ggtagggtgg acgaagattc ggcgggacgc gccaagcgtt atcgcgaaca tgcggcggaa
     1141 atctacgccg cggccgtggg tgttacgcat cctcccagcc gcgcggcttt gctgcgtctg
     1201 gctgaaacct acgatcgctt ggctgctaaa atcgacgcgg aaccgagagc cggtagagcc
     1261 ggtaaaggtc tcttggacga atgatggcgt agccgcttat cgccgccctc gggcgcgcgc
     1321 atcggcgatg agtgcgtgca agcgtgccgg caaaagcggg gtctccgcga tccttaatcc
     1381 gaatggcgag agcgcatttt ctatcgccga catcaccgcc ggtgaaacag ggattacgcc
     1441 cgcttcgccc gctcccttga cgccgagagg gttcatcggc gaaggcgatt gacgatacga
     1501 agtctcgaag cgcggcactt cgggcgcggt cggtatcaga tagtcggcaa agctcgttgt
     1561 ggtcggctgt ccgtgcgaat catagcccat ccattcgtag agcgcgtttc ccagaccgtg
     1621 cgagatgctg ccatgtgttt gtccatcgac aatcatggga ttgatacgga cgccggaatc
     1681 gtgcagggcg agatagcgtg tgacgcggac gtgcgcggtc tcgatgtcga cctccacctc
     1741 cgcgacgtgg cagacattcg cgtaggcaag cagatcggtc cggaaacacg cggtggcctc
     1801 caagccgggc tcgaacccgg gcggaaaggc gtaacccgga ccgccgcgca aaattcgcgc
     1861 gagttccgcg agagcgatag cgcgcgaggg atcggctttt gctcgcaccg taccgtcggc
     1921 gacttcgaga tcgtcaggcg gcatttgcag catcatgccg ccgagctttc tggctttttc
     1981 ggccacggca cgggctgcga ggtatacgga cgatcccgcc gtcaccgtct gccggctggc
     2041 aaaggcgcca agaccccagg gcaccacggc ggtgtcgcca tgaatcacat gcacgtcctg
     2101 gggccgcatg cccaattgct cggcgcagat ttgcgagagt gcggtcacga gcccctgacc
     2161 gatggcggcg gcacccgtca gcaccgatac gacacccgtg ccggtgacgc gcacgacggc
     2221 ggattcgaaa ggtccgcgtc ccgtgccttt gagcccatgc gcgagaccga tgccgatata
     2281 gcggccttct tcgcgcgcga gcttctggcg ctgcggaaaa gactcccacc cggcctgctg
     2341 tagcaattcc gcctggcagg cggggtaatc gccgctatcg taaacgatgg acgcgcccgc
     2401 ccgggatttg atcggttttt cgtagggcag tttttccggc ggtatcagat tcctgcgcct
     2461 gagctcggcc cggtcgatat ccaactcgtg ggccacgcga tccagcagac gttccatcgc
     2521 aaaagcggcc tgcggatacc ccgcgccgcg aacagatgac accggcacct tgttggtgtg
     2581 cgcgaccacc acgtccatat gaagggccgg caccttgtac ggtccgctca cgccggaggc
     2641 ggaattgtag ggcagattca cgtcctggaa cgcataggcg ccctgatcgt ggaacagtct
     2701 gccccggatg cccagcacgt ggcccttgtc gtctaccgcg atttcgatgg tccaatattg
     2761 gtcgcgctca tgcaccgcgc cgacgaaatg ttcacgccgg tcctcgatcc atttgagcga
     2821 gcgcttcaag agatgtgcgg ccgccgtcac cgccacgtct tcgggataga cgcagagctt
     2881 ggggccgaag ccgccgccca catcgggtgt ggcgacgcgc aggcggttct cgtccaatgc
     2941 aagaaaggcg gaaagatttt ggtagagatc atgcgccttt tgggtggagg cgaagaccgt
     3001 caccccgtca ttggccggtt catattgcgc cgccagcccg cgcggttcga tcggatgggc
     3061 agagccgcga tgctgccaga gctcttcctt gaacacatgc gccgcgttgc ggaacgcagc
     3121 gtccgtatcg ccataagtca ctttgtaggt ggcgacgatg ttggaggcga gttcgctgcg
     3181 gacacgaggc gagccggcaa cagccgcctc gcggggatcg gtgacgggcg gcaggatgtc
     3241 gtatgtgaca tccaccagcg ccgccgcgtc ctccgccaga tagcgattct ccgcgacgac
     3301 gagcgcgatg ggctcgccga cgaacgcgac ctcgccgggc gaaagcgcga acggccataa
     3361 ttccgcgcgc ggtgttcctt gcgccggctg ccgcaccatc cgccgtatgc gcaagctggg
     3421 catcagatcg tcgagcgtga gcaccgcatg gatgccgggg gccgaacgcg cggcctcggc
     3481 atcgatgctt aggatcgtcg catgcgcgtg ctggctgcgc acaaacgcca cgtgcaaaat
     3541 gcccggaatc cggatgtcgt cgacaaagcg tccccgcccc aggagcagcg gcaaatcttc
     3601 cagccgccgg aagcgctggc cgatgcggcc ctcctctccg tcataaagtt ttgcgctcaa
     3661 tagatcgcgc gcgcggcttt c
//
LOCUS       DASYEG010000039         4203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4652333, whole genome shotgun sequence.
ACCESSION   DASYEG010000039 DASYEG010000000
VERSION     DASYEG010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4652333"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="VGM72_01540"
     CDS             <1..1006
                     /locus_tag="VGM72_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY4263974.1"
                     /translation="EIALPADLTRGPFAGGILNLPAAAIVLLLMTALFTGVKQSAKMN
                     MAIVAVKLGALAIFILVASLHVNPSLWHPFMPFGWFSHTTDGKTVGVLAGASLVFFAY
                     RGFQTVSAAAEESTNPQRDLPIGIIGSLTICTAIYLSVAGLLTAIAPYMSLNIASPVG
                     FALLQLGYSWGAGLVSVGVVVGLTSTLLILFYCLTRALFAMARDRLLPPFFVTLSPRT
                     GTPINATILVGIVTSSIAGLVPIGALAELVNANTLVEFALVCTGVIVLRITKPDMPRP
                     FKARGGLILPVLGIVSCLALLAFLPVATLQRYVLWLAIGIAFYFLYAARRTGALTAEA
                     "
     gene            complement(1007..1552)
                     /locus_tag="VGM72_01545"
     CDS             complement(1007..1552)
                     /locus_tag="VGM72_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008035437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActR/PrrA/RegA family redox response regulator
                     transcription factor"
                     /protein_id="HEY4263975.1"
                     /translation="MSEIVLPEDKSLLVLDDDRPFRERLARAMQARGFDVKVAETVRE
                     GIAMVKENPPAFAVVDLKLEDGNGLDVVETLHEVRPAARVVVLTGFGNIATAVAAVKF
                     GAVDYLSKPADADDILGALLAAPGGKPSPPENPMSADRVRWEHIQRVYELCGHNVSET
                     ARRLNMHRRTLQRILAKRSPR"
     gene            complement(1654..3036)
                     /locus_tag="VGM72_01550"
     CDS             complement(1654..3036)
                     /locus_tag="VGM72_01550"
                     /inference="COORDINATES: protein motif:HMM:NF033792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActS/PrrB/RegB family redox-sensitive histidine
                     kinase"
                     /protein_id="HEY4263976.1"
                     /translation="MVSIEETAGSSQSPSMFLRFKVPREVRGRVRLGTLNNLRWLAVV
                     GQAVALLVVRLVLNFDFPVLLAASPIAASAVINIILAIAYPAAKRLSAREATAFLAYD
                     IVQLAALLFFTGGIENPFALLFLAPVVISAATLDVLSTLFLGGLSFAAILFLWQAHLP
                     LPWSPVNSFRLSPLYQIGIWVSLLLGIGFTSIYAWRIASEGTRMSAALSATQLALARE
                     HRLGSLGALAAAAAHELGTPLGTIAVVAHELARELATSPHADDFRLLRGEAERCRAIL
                     ARLAQPEESVLGHTERLPVGALLDDIAAPHRGVDVVISLDLQAGPAPRIWRVPEILHG
                     LGNLIENAADFASKEVRLRARWNATALSIEVMDDGPGFAAEIFEQLGEPYITSRPASR
                     KEAHREVNPADGKQEGMGLGFFIAKTLIEQTGGTVTAGNRMGGGAVVTAMWPRGAIDG
                     EALPPQESRL"
     gene            complement(3197..3892)
                     /locus_tag="VGM72_01555"
     CDS             complement(3197..3892)
                     /locus_tag="VGM72_01555"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEY4263977.1"
                     /translation="MGRKIMLGSGAAAVLMLLAACDREPASTTTAPQAAVDATAIQAA
                     QNDPVPGSKNETLSAAKDAVAGAVGTLSAELTTTTKGFVDAAALSDMYEVQAGKLAAM
                     RAHMPELKKFAQDMVDAHTQTTDRLKSILVKSAPDVMPPAQLDSRREDMLNDLKGAKD
                     ADFDGRYIAQQIGAHDEASILMRGYAKDGDNPDIKMFAADTEPKVQMHLDMINGIDRK
                     NRANDEQARNKTP"
BASE COUNT          732 a         1360 c         1289 g          822 t
ORIGIN      
        1 tgagatcgcg cttccggccg atctgacgcg cggccccttc gcgggcggca tcctcaatct
       61 tcccgccgcg gcaatcgtgt tgctgctgat gaccgccctt ttcaccggcg tgaagcagag
      121 cgccaagatg aacatggcca ttgtcgcggt gaaactcggt gcgcttgcca ttttcattct
      181 ggtggcgtcg ctgcatgtga atccgtcgct ctggcatcct ttcatgccgt tcggctggtt
      241 cagccatact acggatggaa agacggtcgg cgtgctcgca ggcgcatcgc ttgtgttctt
      301 tgcctatcgc ggctttcaaa ccgtgtccgc ggccgccgaa gaatcgacaa atccgcaacg
      361 cgacctgccc atcggcatca tcggctccct cacgatctgc acggcgatct atctttcggt
      421 ggcggggctc ttgacggcga tcgctccgta tatgtcgctc aacatcgcgt ctcccgtggg
      481 gttcgcattg ctgcagcttg gttattcgtg gggggcaggg ctcgtttccg tcggtgtcgt
      541 cgtcggactg acatcgacgc tgctcatcct gttctactgc ctgacccgcg cgcttttcgc
      601 gatggcgcgg gaccggctcc tgccgccctt cttcgtgacg ctcagtccgc gcacgggcac
      661 gcccatcaac gcgacgatcc tcgtcggcat cgtgacgtcc tcgattgcgg ggctcgttcc
      721 catcggagcg ctggcggaac tcgtgaatgc caatacgctt gtggagttcg cgctggtctg
      781 tacaggggtt atcgtgctgc gcatcacaaa gcccgatatg ccgcgtccct tcaaggcacg
      841 cggcgggctg atcctgcccg tgctggggat cgtttcctgc ctggcgctgc tcgcgtttct
      901 gcccgtggcg acattgcagc gctatgtgct gtggcttgcc atcggcatcg cgttttattt
      961 tctctatgcg gcgcggcgca cgggcgcgct taccgccgaa gcgtagctaa cgcgggctgc
     1021 gtttggcgag aatccgctgc agcgtgcggc gatgcatgtt gagacgccgc gccgtctccg
     1081 agacattgtg accgcacaat tcatagacgc gctgaatgtg ctcccatcgc acccggtcgg
     1141 cggacatcgg attttccggc gggctgggct tgccgccggg cgctgccagc agcgcaccca
     1201 atatgtcgtc ggcatcggcc ggcttggaaa gataatcgac ggcgccgaac ttcaccgcgg
     1261 cgactgccgt tgcgatgttg ccaaagccgg tcagcaccac gacgcgcgcg gcgggtctga
     1321 cttcgtgaag ggtctccacc acgtcgaggc cgttgccgtc ttccagcttc agatcgacca
     1381 ccgcgaaggc gggaggattc tccttcacca tcgcgatgcc ctcgcgcacc gtctcggcga
     1441 ccttcacgtc aaaaccgcgg gcctgcatgg cacgcgcgag ccgttcccgg aacggacggt
     1501 cgtcatccag gaccagaagg ctcttgtcct cgggaagcac aatttcactc atcgcgtcat
     1561 gctcgcaaca tcgctccttc ggctacatat agcgccggcc gcgatttgac caatcgataa
     1621 agcacccttg cctaatttga tgacgcggtc cggctaaagg cgggattcct gaggaggcag
     1681 ggcctcgccg tctatggcgc cccggggcca catggcggtc acgaccgccc cgccccccat
     1741 gcgatttccc gccgtcactg tgccgccggt ctgttcgatc agcgttttgg cgatgaaaaa
     1801 gccaagtccc atgccctcct gcttgccgtc cgcggggttg acctcacgat gggcctcctt
     1861 gcggctggca gggcgcgagg tgatgtaagg ctcgccgagc tgctcgaaaa tctcggccgc
     1921 gaagccgggg ccgtcatcca tgacctcgat cgagagcgcg gtggcgttcc agcgggcgcg
     1981 cagacgcacc tccttgctgg cgaaatcggc ggcattctcg atcagattgc cgagcccgtg
     2041 caggatttcg ggtaccctcc agatcctggg cgccggaccg gcctggagat ccagggaaat
     2101 gaccacatcc acgccgcggt ggggcgcggc gatatcgtcg aggagcgcgc ccacgggcag
     2161 gcgctcggtg tggccgagta ccgattcctc aggctgggca aggcgggcca ggatggcccg
     2221 gcagcgttcg gcttccccgc gaagcagccg gaaatcatcg gcgtgaggac tcgtcgcaag
     2281 ctcgcgcgcc agctcatggg cgaccaccgc gatggtgcca agcggcgtgc ccaactcatg
     2341 tgctgccgcg gcggccaacg cgcccaagga cccgagccgg tgctcgcgcg ccagcgcgag
     2401 ctgagtcgcc gagagcgccg ccgacatgcg cgtgccctcg ctcgcgatgc gccaggcata
     2461 gatcgaggtg aaaccgatcc ccagaagcag cgaaacccag ataccgatct gatagagcgg
     2521 cgacagcctg aaactgttca ccggcgacca cggaagcggc agatgcgcct gccacaggaa
     2581 cagaatcgcc gcgaaggaaa gacccccgag gaacagcgtg ctcaggacat cgagcgtcgc
     2641 cgccgaaatc accacaggtg cgagaaacaa caaggcaaag gggttctcga tgcctccggt
     2701 gaagaaaagc aaagccgcca gttgcacgat gtcataggcg agaaaagccg tcgcttcgcg
     2761 cgcggagagc cgcttggccg caggataggc gatggcgagg atgatgttta tcaccgcgct
     2821 tgccgcgata ggagaggccg cgagcagcac cgggaaatcg aaattgagca ccaggcgcac
     2881 caccagcagc gcgaccgcct ggcccaccac cgcaagccag cgcagattat tgagcgtgcc
     2941 cagccgcacc cgtccgcgga cttcgcgcgg caccttgaaa cgcagaaaca tgctgggact
     3001 ctgagacgaa cccgccgtct cttcgatcga aaccatggga acctgccggc caaaccacga
     3061 caccggctta tagcagtttc ctcggcgtgc gatggcgatg gataaaaata aagcgccccc
     3121 ggagatgaat ccgggggcgc ttcgcaggct aagcgctggt cagctgggag ggcacggctt
     3181 cacgctcgcc tgtttctcaa ggtgttttgt tgcgcgcttg ctcatcgttg gcgcggtttt
     3241 tccgatcgat gccgttgatc atgtcgagat gcatctgcac cttgggttcc gtatcggcgg
     3301 cgaacatttt gatgtccgga ttgtcgccgt ccttcgcgta accacgcatc aggatcgacg
     3361 cctcgtcgtg ggcgccgatc tgctgcgcga tgtaacgccc gtcgaaatcc gcgtccttcg
     3421 cacccttgag atcgttgagc atatcctcac ggcgcgaatc gagctgcgcc ggtggcatca
     3481 catcgggtgc ggatttcacc aggatcgatt tcaaccgatc ggtggtctgg gtatgagcat
     3541 cgaccatatc ctgcgcgaat ttcttcaatt cgggcatgtg cgcgcgcatg gccgcgagtt
     3601 ttcccgcctg cacttcatac atgtcgctca gcgccgccgc atccacaaaa ccctttgtgg
     3661 tggtggtgag ttccgcggac agcgtgccga ccgcaccagc caccgcatcc ttcgccgcgc
     3721 ttagcgtttc attcttgctt cccgggacgg gatcgttctg tgccgcctga atggccgtcg
     3781 catcaaccgc agcctgcggc gcggtggtcg tcgatgccgg ctcgcgatcg cacgcggcga
     3841 gcagcataag aaccgctgcg gccccgcttc caagcatgat cttgcgaccc atgatatcct
     3901 ccatcttgtc ccgaaaaccc gtgagcccag atttcgttcc gcaaagaaaa cgctccgctt
     3961 cggccatcct tgggtgaaac cgaactggcc ccctgttgcg ccgccgcgtt agtgtagggg
     4021 tgacattttc gagtctttgg catggacgcg atgcttcctt cgagcgcggc cgcgacgcac
     4081 gatctgcgag ggcgggatcc tgccattgcc gtcgaaggac tgatcaaacg ctttggcgca
     4141 ttgaccgcgg tcgatggcat cagcttcact gtggcgcgcg gttcctttac ggcactcctc
     4201 ggg
//
LOCUS       DASYEG010000040         2325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2767988, whole genome shotgun sequence.
ACCESSION   DASYEG010000040 DASYEG010000000
VERSION     DASYEG010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2767988"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="VGM72_01560"
     CDS             complement(<1..1065)
                     /locus_tag="VGM72_01560"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEY4263978.1"
                     /translation="MANARRKIDLDKPRDRMVDRQITARGVRDGRVLAAMRRVPREDF
                     VDEGAEEFAYEDSPLPIGEGQTISQPYIVALMLEAAEICPADRVLEVGAGSGYAAAVA
                     AQLAAHVSAIERHASLVTQAKERFAALGYSNISLRHGDGTKGWPDGGEFDAIVVSAGG
                     SQIPPALKSQLKIGGRLVIPVGPEDEAQELLKLTRRSETEFEEQRLGSVRFVPLIGSE
                     TWVEDGRRSSSSHLPGASQQKTPTELIAETAEPLPDFDDPSFGALFDRFADKRVVLLG
                     EASHGTSEFYRARAAVTRHLIARHGFSILAVEADWPDAAAIDRYVRHLQPREGEPPFQ
                     RFPTWMWRNTDVDAFIDWMRQ"
     gene            1518..1982
                     /locus_tag="VGM72_01565"
     CDS             1518..1982
                     /locus_tag="VGM72_01565"
                     /inference="COORDINATES: protein motif:HMM:NF015444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HEY4263979.1"
                     /translation="MKSQRIASEGTVDTLVVVLDNGEEAFSALQNFARNEGISAASLT
                     AIGAFARATVGWFDFASKSYKEIEIGEQCEVLSAIGDVAVGDDGKASLHVHVVLGLSD
                     GSTRGGHLLKGTVHPTLEVVLTETPARLRRRKRPDLGIALIDADPHRENSRQ"
     gene            complement(1976..>2325)
                     /locus_tag="VGM72_01570"
     CDS             complement(1976..>2325)
                     /locus_tag="VGM72_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263980.1"
                     /translation="AIALEEAGASKYESGGRNDRNLHRTERKEAKGETAQQEREGKSH
                     VGASTKRESTRSVGGKDARKPTARGEKAANARAHRADGHTRDELYARAQRRHIEGRSK
                     MSKRQLENALNVH"
BASE COUNT          404 a          729 c          743 g          449 t
ORIGIN      
        1 ctggcgcatc cagtcaatga acgcatccac gtcggtgttg cgccacatcc aggtcgggaa
       61 acgctggaac ggcggctcgc cctcgcgagg ctgcaggtga cgaacgtagc ggtcgatcgc
      121 cgcagcgtcg ggccaatcgg cctccactgc aaggatggaa aacccgtgac gcgcgataag
      181 atgtcgggtg acggcggcgc gcgcccgata gaattccgac gtgccgtggc tcgcttcacc
      241 cagcagcacg acacgtttat cggcgaagcg gtcgaacaag gcgccgaagg acggatcatc
      301 gaagtcgggg aggggctcgg cggtttccgc gatgagctcg gtcggggtct tttgctgcga
      361 agcgccgggc agatgggacg aagacgatcg ccgcccgtct tctacccagg tttccgagcc
      421 gatcaggggc acgaagcgaa ctgatccgag gcgttgctcc tcgaattctg tttcggaacg
      481 gcgtgtcagt ttgagaagct cttgcgcctc gtcctcaggg ccgacgggaa tgacaagcct
      541 gccaccgatc ttcagttggg atttcagcgc aggcggaatc tgcgatccgc cggccgaaac
      601 cacgatggcg tcgaattcgc caccatccgg ccagcccttt gtcccgtcgc cgtgtcgcag
      661 gctgatattg gagtagccca gcgccgcgaa ccgctcttta gcctgcgtga ccaagctcgc
      721 gtgccgctcg atggcactga catgcgctgc gagttgcgcc gctacggcag cggcatagcc
      781 cgagccagcg ccaacctcca aaacgcggtc ggccgggcag atttccgccg cctcaagcat
      841 gagcgcgaca atgtagggct gcgagattgt ttggccttcc ccgatcggca gcggactgtc
      901 ttcgtaggca aattcctccg cgccttcgtc gacgaaatcc tcccgcggaa cgcggcgcat
      961 cgcggcgagc acgcggccgt cccgcacgcc ccgggccgtg atctggcggt ccaccatgcg
     1021 atcacggggc ttatcgaggt cgatttttct tcgcgcattg gccatcggaa gaaaccgctg
     1081 ccggcccggc cagttcccca tggttcgctg ccctgaacgc cagaaaggaa ccacgtgagt
     1141 tgcgtggcgt tgctgtggag acgcgcacgc gaggttcaaa atggctctgt cccaagacat
     1201 gaaacgctgc atcgatgttt gcctggattg ctataaaacg tgccttggca cagccatgaa
     1261 ccactgcctg gaaacgggcg gcgagcacgt cgcgccagcg cacttccggt tgatgatggc
     1321 ctgcgccgaa atgtgccgaa cgtcagcgca cttcatgctc atctcctcgc cccaccacaa
     1381 gcacacctgt cgcgaatgcg cggagatctg cgcggagtgc gcgaaggatt gcgagcgcct
     1441 tggcggaatg gatgagtgcg tggccgcctg tcgggcctgc gccgagtcat gcaccgcgat
     1501 ggcgggttga gacgagcatg aagagccagc gcatcgcgtc cgaaggcacc gtcgatacgc
     1561 tcgtcgttgt cctggacaat ggtgaggaag cattctctgc tctgcagaac tttgcccgga
     1621 acgagggaat ttccgcggcg tccctgacgg cgatcggcgc cttcgcgcgc gcgacggtcg
     1681 gatggttcga cttcgccagc aagtcgtaca aggagatcga gatcggcgaa cagtgcgagg
     1741 tgctgagcgc catcggcgat gttgctgtcg gggacgacgg gaaggcgagc cttcatgttc
     1801 acgtcgtgct ggggctttcg gacggatcaa cccgtggtgg ccatctcctg aaaggcaccg
     1861 ttcatcccac actcgaagtc gtcctgactg aaacgccggc gagattacgg cggcgcaagc
     1921 gcccggatct cggcatcgcg ctgatcgacg ccgatccaca ccgggagaat tcgcgtcagt
     1981 gaacgtttaa tgcgttttcc agctggcgtt tgctcatttt tgagcggcct tcgatgtggc
     2041 ggcgctgcgc ccgggcgtag agctcatcgc gggtgtggcc gtcagcccgg tgtgcgcgcg
     2101 cattcgcagc tttctcgccg cgggccgtcg gtttccgagc atcttttcct ccaaccgagc
     2161 gcgtgctttc gcgtttggtc gaagcgccaa catgcgactt tccctcgcgc tcttgctgtg
     2221 cggtctcgcc cttggcttct ttccgctcgg tgcgatggag attgcgatcg ttgcggcccc
     2281 cgctctcgta cttcgatgcg ccggcctcct ccagtgcgat cgcga
//
LOCUS       DASYEG010000041         1939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1608128, whole genome shotgun sequence.
ACCESSION   DASYEG010000041 DASYEG010000000
VERSION     DASYEG010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608128"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="VGM72_01575"
     CDS             <1..1040
                     /locus_tag="VGM72_01575"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEY4263981.1"
                     /translation="RWKRAITAVSGDDFGVGDRFGTFGTMGFGVGQLYTAKWFGPDAK
                     AKIETLVQNLLAAYRVRLQNLDWMSDATKVEAIKKLDTYTIKVGYPDHPRDYSGLVVL
                     DDDLVGDVRRAGAFDWAFYLGRYNGPVDRSDWQMTPQTNDAYNGSLRDIVFPAGILQA
                     PIFDANADAAINYGAVGGVIGHELTHGFDDEGRKIDAEGRLRDWWTKDDAAKFEARAK
                     VLGAQYSSFEPLPGVHVNGDLTMGENIADLGGVTLALDAYHTSLKGHRARKVDGYTGD
                     QRVFLGWAQAWRGKVSDDYVKKQVVSDPHSPRQFRVNGPVRNVDAWYESFDVKPTDKL
                     YVAPDARVHIW"
     gene            1100..1804
                     /gene="pgl"
                     /locus_tag="VGM72_01580"
     CDS             1100..1804
                     /gene="pgl"
                     /locus_tag="VGM72_01580"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEY4263982.1"
                     /translation="MRDRLCEFPDAGSMIANLADNIVRRLSAAVQDRGRASFVVSGGT
                     TPGALFDVLSTRDAPWDKIWITLSDERWIAPTEDGSNEKLVRTRLLCGKAATAHFVAM
                     KTEDVKPEDAQAAVGAAIAAMPHPFDVTLLGMGDDGHTASLYPQAAELKAALDTKAPA
                     LVHAVHAKNAAATGDRMTLSLRAILDSQWIVVLIKGAAKMKTYRDAEEGADPLKMPVR
                     SVLAQADVPVEVFWAP"
     gene            complement(1822..1898)
                     /locus_tag="VGM72_01585"
     tRNA            complement(1822..1898)
                     /locus_tag="VGM72_01585"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1862..1864),aa:Arg,seq:acg)
BASE COUNT          383 a          585 c          621 g          350 t
ORIGIN      
        1 tgcgctggaa acgcgccatc accgccgtca gcggcgatga tttcggcgtc ggcgatcgct
       61 tcggcacgtt cggcaccatg ggcttcggcg tcggccagct ctacaccgcc aaatggttcg
      121 gccccgacgc gaaagccaaa atcgaaaccc tggtgcaaaa tctgctcgcc gcctatcgcg
      181 tgcgtttgca gaatctcgac tggatgagcg acgccaccaa ggtcgaagcg atcaagaaac
      241 tcgataccta cacgatcaag gtcggctatc cggatcaccc gcgtgactat tcgggccttg
      301 tcgtcctcga tgacgatctc gtcggcgatg tgcgccgcgc cggcgccttc gattgggcat
      361 tctatctggg ccgctacaac ggtccggtcg atcgcagcga ctggcagatg acgccacaga
      421 caaacgacgc ctataacggc tcgctacgcg atatcgtctt cccggcgggc attcttcagg
      481 cgcccatctt cgatgccaat gccgatgcgg ccatcaatta cggcgcggtg gggggcgtca
      541 tcggtcatga actgacgcac ggcttcgacg atgaaggccg caagatcgac gccgaaggcc
      601 gcctgcgcga ctggtggacg aaagacgacg cggcaaagtt cgaagcgcgg gcaaaagttc
      661 tgggcgcgca atattccagc ttcgagccgt tgcccggcgt gcatgtgaac ggcgacctga
      721 cgatgggcga gaacatcgcc gatctgggcg gcgtcaccct ggcgctcgac gcctatcaca
      781 cctcgctcaa aggccatcgc gcgcggaagg tcgatggtta cacgggcgat cagcgcgtgt
      841 tcctcggctg ggcgcaggca tggcgcggga aagtctcgga tgattatgtg aagaagcagg
      901 tcgtcagcga tccgcacagc ccgcggcagt tccgcgtcaa cggcccggtg cgcaatgtcg
      961 atgcctggta cgaatccttc gatgtgaaac cgaccgacaa actttatgtc gcgccggacg
     1021 cccgcgtgca tatctggtga cgggagcttt gcgcggctgc ccgcgttggg tctcgtgatg
     1081 atctgcgagg accgaaccaa tgcgtgaccg gctgtgcgag tttcccgatg ccgggagcat
     1141 gatcgcaaat ctggcggata acatcgtccg cagattaagt gcggcggtgc aggatcgcgg
     1201 gcgcgcaagt tttgtcgttt cgggtggaac cacgcccggt gcgcttttcg atgttctctc
     1261 cacgcgcgac gccccgtggg acaaaatctg gataacgctg tcggacgagc gttggatcgc
     1321 gccaacggaa gatggcagca atgagaaact ggtgcgcacc cgcctgctgt gcggcaaagc
     1381 ggccaccgcg catttcgtgg cgatgaagac cgaggatgtg aaacctgaag acgcgcaggc
     1441 tgcggtcggt gcggccatcg ccgcaatgcc ccaccctttc gatgtgacac ttctgggcat
     1501 gggcgacgac ggccacaccg catcgcttta tccccaggcg gccgagctga aggccgcgct
     1561 cgatacgaag gctccggcac tggtgcatgc cgtccacgcc aaaaacgcgg cggcgaccgg
     1621 cgatcggatg acgctgagct tgcgcgccat tctggattcg caatggatcg tcgtcctcat
     1681 caagggcgcg gcaaagatga agacctatcg ggatgcggaa gagggtgccg atccgttgaa
     1741 aatgccggtg cgcagcgtgt tggcgcaggc ggacgtgccg gtcgaggtgt tctgggcgcc
     1801 ttaaatcgtc gcaatggaaa gtggcgcgcc cgaaaggatt cgaacctctg acccccagat
     1861 tcgtagtctg gtgctctatc cagctgagct acgggcgcgc atgtcgcgag ggcgcgcaac
     1921 ctattcagcg aaaacccca
//
LOCUS       DASYEG010000042        12367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_594051, whole genome shotgun sequence.
ACCESSION   DASYEG010000042 DASYEG010000000
VERSION     DASYEG010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 12367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12367
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594051"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="VGM72_01590"
     CDS             complement(<1..1046)
                     /locus_tag="VGM72_01590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4263983.1"
                     /translation="MYRIVNGSRIMAYLRNSAVNLLNLHYGMHAIALNGAGVFFFVFL
                     LKAGVPVPAVFIAIALTVAARFLFRPLILVLAPRCGLRALVAFGTMFTGVQYLFLARV
                     QGVDLMLLGFCLSAAIGDVFYWTCYHAYFAALGDSEHRGHQIGAREALGSFAAIAGPL
                     IGAWALTTLGPRIAFGAAACVHVLAALPFLGTPDVKVARMVPRVFRAAIPGILLFAAD
                     GWIAAGFLFVWQIALFLSLGESFSAFGGALALAGLVGAIGGLLLGRHIDAGHGGRAVW
                     LVFASIAGVTLFRAASTGNAAMAVTANALGTLAGCLYIPTLMTAVYNQAKRSPCALRF
                     HVATEGGWDIGCAAG"
     gene            1271..2119
                     /locus_tag="VGM72_01595"
     CDS             1271..2119
                     /locus_tag="VGM72_01595"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY4263984.1"
                     /translation="MRFPRPANMLAALSPRRAAPMLAAGLLLPLLAGQAAATPTTTWN
                     LYTNCTQIAGSGSSCVGDGSNHASNGNEYRFSSATSPNLSLTAAAYHSNKNTSLGHAS
                     FLGEYTGSQFGLGVENDNAPEHAVDNNGGYDFIVFRLPTGSNFDQLKITLSPFGWTED
                     MNATILYGTPTAGLGIGLDPSVTAFKNLSIDDLLDNGFSMETTTNLLDKSTPQTISLS
                     SPDIINYFIVAASLTPGRHEDDYFKVNAVTAIDSPSPVPEPASLALLAAGLFGLFTLR
                     QRAVKA"
     gene            2407..2805
                     /locus_tag="VGM72_01600"
     CDS             2407..2805
                     /locus_tag="VGM72_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263985.1"
                     /translation="MSVKLPSHFDFGIDLGMDISGIPTNYSFGITALPKIDIGIEPIT
                     LSIDPLEIKPLDLSFRIKEIPSVRVHLPVDYKVGVALFGAELASVRLCGQAQIITEPY
                     VPNPCECRGAVNRGNIMDGRDVPAPIKAAG"
     gene            2817..3536
                     /locus_tag="VGM72_01605"
     CDS             2817..3536
                     /locus_tag="VGM72_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263986.1"
                     /translation="MAKRSAAHAHPLSFRGTPRSLSLSNPALAGHECGELVLPEELAA
                     HSESAVIKLPVHSRRSDGPSLKLRLDRGAPAGKYQAQLRLAGKSVPVTLDIAPAPRLS
                     VFPLSADFTGKAGSDCELEMTLVNKGNVAIELPERSIAGIFDDEGLEAAFAETYRQDT
                     DDPVQLVGHWLRKLRDGYGGLLKLRIGSGSVSLPPDTERTITVSAHLPESLKRGHSYH
                     GIWEIGPVHYRVTVAISKDKG"
     gene            3539..4174
                     /locus_tag="VGM72_01610"
     CDS             3539..4174
                     /locus_tag="VGM72_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263987.1"
                     /translation="MTITPCSGTTPIDTGDLQKLLKDVVAAQFSLGKEMLKVLGNGTN
                     SALAGMRGLNMPNLPKLHTCCDIPEPCWMPKSLGEITCQLTAGSTGQVKLIVTNNDFR
                     AHTAIAQSAGKDAALVQFTSSQVALGPKERTTITAQFTAPPDAGTYEFLIWVSVCSDH
                     YLRVTVTVGDTNNACCYQVTVDDNPNYVVHWYDHFYCPRPCPGTLAKQANG"
     gene            4175..4717
                     /locus_tag="VGM72_01615"
     CDS             4175..4717
                     /locus_tag="VGM72_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263988.1"
                     /translation="MKDRLLDVLKGAVEVNLRYSSIALNLSKEYIKEFDRTLRDGAAV
                     KPEPNSEPAPETRPASARRQPILLVGQLDEEAGGAFVLNNTSESELNVSLTVQGELDP
                     DQVHLVPAAFVLAAGASAFVRLKITLTGALEENRDYSGAVFAPGLSAQAIAFVVRRLP
                     GEGMAKPAKKGKASAKRATG"
     gene            4724..6556
                     /locus_tag="VGM72_01620"
     CDS             4724..6556
                     /locus_tag="VGM72_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY4263989.1"
                     /translation="MNAGDFQRRGEEFSRRLFEFLQRTQAPGRSGEEADPSAVAKPLL
                     SLIDSATKGLNELREMVAGANAPPQNPGEAQNPLHGKGFLQTLKLLIAHVLNAPESTA
                     KHYAQFAGEALRIFDGESDLKPEQRDFRFKDRLWLDSHFFRVLLQLYLAWGHSVQSWL
                     DEQELDEADKRRINFILQQVIAAIAPSNLPLNPSALRRADTTEGRSMVDGVRHWIDDA
                     IHNRAMPRQIRRGAYEVGKDFALTPGAVVHRSEQLELIQYAPQTERVRRRPVLLIPAQ
                     INKYYCFDLRPQNSFVAHAVRAGIQLFIVSWRNPNAEHAHWNLDTYARATLDAMNAVS
                     AITKSRTMGVISACAGGLTAMAAMGYLAARGERRVVNHSLLVTCLFPNQGSDLELFVT
                     PDAVELARSHVRANGVMDGEELAKLFFWLRPTDLVWRYWINNYLLGKNPPPLDVLFWD
                     NDSTRLPAALHSDFLDMYTNDVFHRPNAIEVLGQRLDFRKLRVDSYVVGGEDDYLMPW
                     KGCYHACQTFKGRHEFILSTSGHVQSLLRPPRLANSYYFTNRDCDMTPQAWRASCVRQ
                     EGTWWGHWHAWLNSVSGDLKRAPMQLGSTDHPELMPAPGSYVFG"
     gene            complement(6565..8352)
                     /locus_tag="VGM72_01625"
     CDS             complement(6565..8352)
                     /locus_tag="VGM72_01625"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4263990.1"
                     /translation="MAIKNNISRRDLIKTGTLAGAAAAAGGLAATPAAAQAQALPTRW
                     DREADVVIIGAGATGMPAAIVAREAGSSVIIIEAEPDVGGHAICSGANIPLGGGTSIQ
                     KKAGIQDSPDLVFRDLTDWSLTEPNGAADYRFNDREIIRAFADNCAPTFEFLLQHGVK
                     FVNEKPDGRAGSAHGNSVPRQMHVAVLDWPEVETGKQLPPEQRATASNGNGLMRPLEA
                     AARKAGVEILLEHRMTAIYREQPKSGRVLGIAVTNNGRVLNVRAKKAVIVATGGSSGN
                     VNFRRMFDPRLTEEYCGLAGMPWSNQDASGELAGMAINASLWGAANYTLEIGDNITKP
                     GKIGAQYIYSNLTWMPGSRVFDRAGASGLRVKDFQNLIHVNMLGKRIYDETGGQYPGN
                     RYNTVQPYFPDSPKNAKNVKFDAANWIPAAMAGIGDGKNGGGPIWAIFDGDAAAREKW
                     TTEHPWVDTKNGFFFSADTLSGLASKIQMKYQRVPMPSANLEATVARYNSFVDTGADE
                     DFGKPKPLYKIAKPPFYAAWSTPVVHDSRAGLRINAKCQVVDMNGEVIPNLYCGGETA
                     GGFSMHGLARCLCQGYIAGHNAHTEKASA"
     gene            8517..9107
                     /locus_tag="VGM72_01630"
     CDS             8517..9107
                     /locus_tag="VGM72_01630"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY4263991.1"
                     /translation="MRLCTVMAAATFATSSAYAAATYEGSGRVPEQFVLSGKAAERLH
                     DYVSINLDTAEKLVAACQASARKINSPVVVVVLDPQGLPVLESRMDGEGWIQIKAAEQ
                     KTLTALRTRAPSHVLTNRNVQNPFTSQNVAGYGLTANKGGLPIIVNGQLIGAMGAGGM
                     ATPSGEESCVRDALEQVIGPQPPLLADVTPQRNNAR"
     gene            9288..10142
                     /locus_tag="VGM72_01635"
     CDS             9288..10142
                     /locus_tag="VGM72_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4263992.1"
                     /translation="MIDRIRRNILATGAAVTAMAAAPRAFSQPAGQAGAFYEKGPVRI
                     YYEQEGSGFPLLLIAGGGLNSTISGLRRGDPFNPFEEFKADYHCVAADLRNANAGQSS
                     GPLEIDRPWDSHTDDHLGLMDHLGIQKFMVMGFCIGGPLIWNLLKRAPDRVVAAVLAQ
                     PSGFRPEMPTLTYDGNMAGWGPDLVKRRPDITMDTVQKFLTRMYRNNPDFVITVTRDF
                     VRNCQTPVLILPDDIPAHPYAVAMEAAMLAPKAEVSMFPWKQPKERIPLAVRQIRSFL
                     KAHRPVSV"
     gene            complement(10173..11012)
                     /locus_tag="VGM72_01640"
     CDS             complement(10173..11012)
                     /locus_tag="VGM72_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263993.1"
                     /translation="MSKTGFALSAMVAAVLMSASAGPILAANVGASDEGAPDLSGLWV
                     RTGEPMFEPIPGAKEGKPIARLEVNSKDAEEIMAGNWDNPILQPWARAIVKKNADSEV
                     ALKHVYQADDSCWPVTVPAILNMREAVQFLQNKDRVTILYQRDHQIRRVWLNQKHSAK
                     VEPSWYGESVGHYEGDTLVIDTIGVKTHAMSFVDPFGTPHTAKLHVVERYRPIKDEKG
                     KGLDVTIRVEDPGTFTGPWAGRLTYRVNRSQTMEEVICEENNVTFNGQSFGPLPEEKS
                     PQF"
     gene            11157..>12367
                     /locus_tag="VGM72_01645"
     CDS             11157..>12367
                     /locus_tag="VGM72_01645"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY4263994.1"
                     /translation="MMEQKNTAPRIAYGDLREWLALADRLGEVRHVAGASWQEDIGLA
                     AEAILRAENGPCVLFDDVPGCAKGFRLLLNMFAGTRRNMTLGFPYLLTKWELSDAYRA
                     AYLANPKLVPHEIVADGPVLENVLTGDAVDVLKFPAPVWHEKDGGRYIGTGTFSITRD
                     PEEGWLNAGAYRAQVHDAKSVGLVMAAGHHGGQHYEKYQKRGEPMPVAMVLGGDPLSF
                     FYGGLEVPYGTFELDVVGGLRGRPVKMVRGRITGLPFPAAAEIVLEGYVQPDKTRIEG
                     PFGEWTGHYAGGAKPWPVLDVHAIYHRNDPILVGVPPMGAGPDEMARYRAVMRSATIK
                     ENMIRAGVPGVEQVWCHEVGAARSFHGVSIKQRYPGHAVQAGTIAAQCGGSAYASKYI
                     VVTDEDVDVTNL"
BASE COUNT         2407 a         3862 c         3776 g         2322 t
ORIGIN      
        1 cccgccgcgc agccgatgtc ccagccgcct tcggtcgcga cgtgaaagcg cagcgcgcag
       61 ggcgagcgct tggcctgatt gtagaccgcc gtcatcagcg tcgggatgta gaggcacccg
      121 gccagcgtcc ccagcgcgtt cgccgtcacc gccatcgcgg catttccggt gctggcggcc
      181 cgaaacagtg tgacgcccgc gatagatgca aacacgagcc agacggcgcg gccgccatga
      241 cccgcatcga tgtgccgtcc gagcagcagc ccgccgatgg cccccaccag acccgccaag
      301 gcgagcgcgc cgccaaaggc ggagaagctt tctccgagcg agaggaacag cgcgatctgc
      361 cacacgaaca ggaacccggc ggcgatccag ccgtccgccg cgaacaacag gatgcccggt
      421 atcgccgcgc ggaacacacg cggcaccatg cgcgcgacct tgacgtcggg tgtgccgagg
      481 aacggcagcg ccgccagcac atgcacgcag gcggccgcgc caaaggcgat ccggggtccg
      541 agcgtggtca gggcccatgc gccgatcaag ggaccggcga tcgcggcgaa cgaacccaat
      601 gcttcgcgcg cgccgatctg atggccgcga tgttcggaat ctcccagcgc cgcgaaatag
      661 gcgtgatagc aggtccaata gaacacatcg ccgatggccg cgctgaggca gaaaccgagc
      721 agcatgagat cgacgccctg cacccgggcg aggaaaagat attgcacgcc ggtgaacatc
      781 gttccgaacg ccaccaaagc ccgcaacccg cagcggggag cgagaacgag gatcaagggc
      841 cggaacagga agcgtgccgc aaccgtcagc gcgatggcga tgaagaccgc cggcaccggc
      901 accccggctt tcagcaggaa cacgaagaag aacacgcccg cgccattcag cgcgatggcg
      961 tgcatgccat agtgcagatt caagagattg acagcgctgt tgcgcagata cgccatgatc
     1021 cgggagccgt taacgatccg atacacggga cgtagacgtt gccccaccgc ggcgcaaggc
     1081 cggttcgaca cgcaaaagcg gcggcgatcc cgcacgtcgg acgttccgaa aattttccgg
     1141 aattaatact taaatgcgat tcaaattgga cacgcagatt ttcgcctcta tccgttgcgc
     1201 catggaaaac cgagaaaaac acgtgcgctc cgaactggtg ccgcaggatt ttaaacagga
     1261 gaaaatggcc gtgagatttc cccgccccgc aaatatgctc gctgctctgt cgccgcgccg
     1321 cgctgcgccg atgctggcag ccggcctcct gcttcccttg ctcgccggcc aggctgccgc
     1381 aacgccgacc accacctgga atctttacac gaactgcacc cagattgccg gatcgggaag
     1441 ttcctgtgtc ggcgatggca gcaaccatgc cagcaacggc aatgaatatc gtttctcgag
     1501 cgcgacctct ccgaacctca gtctgacggc ggcggcctat cattcgaata agaacacgag
     1561 cctgggccat gccagtttcc ttggcgaata caccggcagc caatttggcc tgggcgtgga
     1621 gaatgacaac gcgccggaac acgcggtcga caacaatggg ggatatgatt ttatcgtctt
     1681 ccgtctgccg acgggctcga atttcgatca gttgaagatc actctcagtc cctttggctg
     1741 gacggaggat atgaacgcca cgatccttta cggcacgccg accgcgggtc tgggcatcgg
     1801 tctggacccc agcgtcacgg cattcaaaaa cctgagcatc gacgatcttc tggacaatgg
     1861 tttttcgatg gagaccacaa ccaatctgct cgacaaatcc actccgcaga cgatttccct
     1921 ctccagcccc gacatcatca attatttcat cgtggcggcg agcctgacgc ccggacgcca
     1981 tgaagatgat tatttcaagg tcaatgcggt gacggctatc gactcgccga gcccggtccc
     2041 cgagcccgcg tccttggcgc tgctcgcggc aggtcttttc ggtctgttca cattgcgcca
     2101 acgcgccgtc aaagcctgat ccggcacgaa aatcatcgca ggatgtggaa gcggcggcgc
     2161 aagccgccgt ttccatttgc gcaaagcgcg cgcctccagg gcgccacatc gccatcgacg
     2221 cgatccatgt ttcgcgaaat cgggtgctga aaaaatgggc aaatcgaatt ccgtgggcga
     2281 gcgatggtct caccggctga ttacaagtgc gaaaggacac gcgaaagcgc atcggtgaaa
     2341 ttgcatccta tcgaacatcg ttctatggtt aacgactgct cgctgattgc gatggggtgc
     2401 tggaacatga gcgtaaaact tccctcgcat ttcgatttcg gcatcgacct cggaatggac
     2461 atcagcggca tcccgacaaa ttattcgttc gggatcacgg cgcttccgaa aatcgacatc
     2521 ggtatcgaac cgatcacgct cagcatcgat cccttggaaa tcaaacccct ggatctgtcg
     2581 ttccggatca aggaaattcc gtctgtccgg gtgcatttgc cggtcgatta caaggtcggc
     2641 gttgcgctgt tcggcgcgga actcgccagc gtgcggctct gcgggcaggc gcagatcatc
     2701 accgaaccct atgtgcccaa tccgtgcgag tgcagaggcg ccgtgaaccg gggcaacatc
     2761 atggacggac gcgatgttcc cgctccgatc aaggccgccg gctaggagac gtgaacatgg
     2821 ccaagcgatc cgccgcccat gcgcaccctt tgagttttcg cggcacgccg cgaagtctgt
     2881 ctttgtcgaa tccggcactc gccggacatg aatgcggcga actcgttctg cccgaggaat
     2941 tggctgccca cagtgaaagc gcggttataa aattgccggt ccacagccgc cgctcggacg
     3001 gcccgtcgct caagcttcgt ctcgatcgcg gagcacctgc cggaaaatat caggcgcaat
     3061 tgcggcttgc cggcaaatcc gttcccgtga cgctggacat agctccggcg ccgcgtctga
     3121 gcgtctttcc gctgagcgcc gatttcaccg gcaaggcggg aagcgattgc gaacttgaga
     3181 tgacgctcgt caacaagggc aatgtcgcga tcgagctgcc cgaacgttcg atcgccggca
     3241 tcttcgacga cgaagggctg gaggctgctt tcgcggagac gtaccgtcag gatacggacg
     3301 atccggtgca attggtcggc cattggctgc ggaaactgcg cgacggctat ggcgggctgc
     3361 tcaagctgcg catcggttcg ggcagcgtct cgctgccgcc ggacaccgaa cgcaccatca
     3421 ccgttagcgc gcaccttccc gaaagcctca agcggggcca ttcctaccac ggcatttggg
     3481 aaatcggccc ggtgcattat cgcgtcaccg tcgctatttc aaaggataag ggttgaccat
     3541 gacgatcact ccctgctcgg gcacaacgcc catcgatacc ggcgatcttc aaaagctcct
     3601 gaaggatgtc gtggcggcgc aattctcgct cggcaaggaa atgctgaagg tgctgggcaa
     3661 cggcaccaac agcgcgctgg cggggatgcg cgggctgaat atgccgaacc tgcccaagtt
     3721 gcacacctgc tgcgacattc cggagccctg ctggatgcca aaatcattgg gcgagataac
     3781 atgccaattg acggccggaa gcaccggcca ggtgaaactc atcgtcacca acaatgattt
     3841 ccgcgcgcac accgcgattg cgcaaagtgc gggcaaggat gcggccctgg tgcagttcac
     3901 atcctcgcag gtggcgctcg gtccgaagga acgcaccacc atcaccgcgc aattcaccgc
     3961 accgccggac gccggtacct acgagtttct gatctgggtt tcagtctgca gcgatcatta
     4021 tctgcgcgtg accgtcacgg tcggcgacac caacaacgcg tgctgctatc aggtgacggt
     4081 cgacgacaat cccaattatg tcgtgcactg gtacgatcat ttctattgcc caaggccgtg
     4141 tcccggcaca ctcgcaaagc aggcgaacgg ttagatgaag gaccgccttc tcgatgtgct
     4201 gaaaggcgcg gttgaagtga atctgcgcta ttcgtcgatt gccctcaatc tatccaagga
     4261 atacatcaag gaattcgatc gcacccttcg cgatggcgcg gctgtgaagc ccgagccaaa
     4321 ctccgaacct gcgcccgaga caagacccgc gtccgcaaga cgccagccga tcctgctggt
     4381 gggccagttg gatgaggaag cgggcggcgc ctttgtgctc aacaacacat cggagagcga
     4441 actcaatgtc agcctcacgg tgcaaggcga actcgatccc gatcaggttc atctcgtgcc
     4501 ggcggccttc gttctggcgg cgggcgcgag cgcattcgtc cgcctgaaaa tcaccctcac
     4561 cggcgcattg gaagaaaacc gggattattc cggtgcggtg ttcgcgcccg gcctgtcggc
     4621 gcaggccatc gcgttcgtcg tacgccgcct accgggcgaa gggatggcca aacccgcaaa
     4681 aaaaggaaaa gcatcggcca aacgcgcgac gggctgagcg gccatgaacg ccggcgattt
     4741 ccagcggcgg ggcgaggagt tttcgcggcg tctctttgaa ttcctgcaac ggacgcaggc
     4801 cccaggccgc tccggcgagg aggccgatcc cagcgcggtc gccaagccat tgctgtctct
     4861 gatcgacagc gcaaccaaag gcctgaacga gttgcgcgag atggttgcgg gcgcgaacgc
     4921 accgccgcag aacccgggcg aggcgcaaaa cccccttcac ggcaagggct tcctccagac
     4981 cctcaaactc ctcatcgccc atgttctgaa cgcgcccgaa agcaccgcca agcattacgc
     5041 gcaattcgcg ggcgaggcgc tgcgtatctt cgacggcgaa tcggacctca agcccgagca
     5101 gcgcgatttt cgtttcaagg acaggctgtg gctcgacagt catttcttcc gcgtgctgct
     5161 acagctttat ctggcctggg gtcattcggt gcagtcctgg ctcgacgaac aggaattgga
     5221 tgaggccgac aaacggcgga tcaatttcat cctgcaacag gtgatcgcgg ccatcgcgcc
     5281 gtcgaacctg ccgctgaacc cgtccgcctt gcggcgcgcc gacactaccg aaggacgcag
     5341 catggtggat ggcgtccgcc actggatcga cgacgcgatc cacaatcgcg cgatgccgcg
     5401 gcaaatccgg cgcggcgctt acgaggtcgg caaggatttc gcgctgacgc cgggggcggt
     5461 cgtgcatcgc agcgaacagc tcgaactcat ccagtacgcg ccgcagaccg aacgcgtgcg
     5521 ccgccggccg gtgctgctga tccccgcaca aatcaacaaa tattattgtt tcgatctccg
     5581 gccccagaac agcttcgtcg cccatgctgt ccgcgcgggc attcaattgt tcatcgtgag
     5641 ctggcgcaac ccgaatgcgg agcacgcgca ttggaatctc gatacctatg cgcgtgcgac
     5701 gctcgatgcg atgaacgcgg tcagcgccat caccaagagc cggaccatgg gcgtgatcag
     5761 cgcctgcgcg ggcgggctga cggcgatggc ggcaatggga tatctcgcgg ccaggggcga
     5821 gcggcgcgtg gtcaatcatt cgctcctggt cacttgcctt tttcccaatc agggctccga
     5881 cctcgaattg ttcgtgacgc ccgatgccgt agaattggca cgcagccatg tgcgcgccaa
     5941 tggcgtcatg gacggcgaag agctggcgaa actgtttttc tggctcaggc cgaccgatct
     6001 cgtgtggcgc tattggatca acaattacct tctgggcaag aatccgccgc cgctcgatgt
     6061 gctgttctgg gataatgatt ccacccgcct gcccgccgcg ctgcacagcg attttctgga
     6121 catgtacacg aacgatgtct tccaccgtcc gaatgcgatt gaggtgctgg ggcaaagact
     6181 cgacttccgc aaactacggg tcgattccta tgtggtcggc ggcgaggacg attatctgat
     6241 gccgtggaaa ggctgctatc acgcctgcca gacgttcaag ggccgccatg aattcatcct
     6301 cagcaccagc ggtcatgtgc aaagcctgct gcgcccgccc cgcctcgcca attcctatta
     6361 tttcaccaat cgcgattgcg atatgacgcc gcaggcttgg cgcgccagtt gcgttcggca
     6421 ggaaggcacg tggtggggac actggcatgc atggctcaac agcgtttcgg gcgatctcaa
     6481 acgcgcgccc atgcagctcg gaagcacgga tcatcccgaa ctgatgcccg cacccggttc
     6541 ctatgtgttc ggctaggcgg ggtcttacgc cgacgctttt tccgtgtgcg cgttgtgccc
     6601 ggcgatgtaa ccctggcaga ggcagcgcgc cagcccgtgc atggaaaacc cacccgccgt
     6661 ctcgcccccg caatagaggt ttgggatcac ctcgccattc atatccacca cctggcattt
     6721 cgcattgatg cgcagccctg cgcgcgaatc atggacgacg ggagtcgacc acgccgcata
     6781 aaacggcggt ttggcgattt tatagagcgg cttgggcttg ccgaaatcct catcggcccc
     6841 cgtgtctacg aaggaattgt agcgcgcgac ggtggcctcc agattggcgg atggcatcgg
     6901 cacgcgctga tatttcatct ggatcttcga agcgaggccg gagagcgtat cggcgctgaa
     6961 gaagaaaccg ttcttggtgt cgacccaagg atgttccgtc gtccattttt cgcgcgccgc
     7021 cgcatcgcca tcgaagatcg cccagatcgg tccgccgcca ttcttgccgt cgccgatccc
     7081 cgccattgcc gccggaatcc agttggcggc atcgaatttg acgttcttgg cgtttttcgg
     7141 gctgtccggg aaatagggct gcaccgtgtt gtagcggttg ccgggatatt ggccgccggt
     7201 ctcgtcgtag atacgcttgc ccagcatatt gacgtggatc agattctgga aatctttgac
     7261 gcgcagaccc gacgcgcccg ctctatcgaa cacccggctt cccggcatcc aggtgagatt
     7321 ggaatagata tattgggcgc cgatcttgcc cggtttggtg atgttgtcgc cgatttcgag
     7381 cgtataattc gcagcccccc agagcgatgc gttgatcgcc atgccggcga gttcgccgct
     7441 cgcgtcctga ttggaccacg gcatcccggc gaggccgcaa tattcttccg tgaggcgcgg
     7501 atcgaacatc cggcggaaat tcacattgcc cgacgagccg ccggtcgcga cgatcaccgc
     7561 tttcttcgcg cgcacattca gcacccgccc attgttggtg accgcgatcc cgagcacgcg
     7621 ccccgatttc ggctgctcgc gatagatcgc cgtcatgcga tgctcgagca ggatttcaac
     7681 accggccttg cgtgccgccg cttccaacgg ccgcatcaga ccattcccgt tagaggccgt
     7741 cgcgcgttgc tccggcggga gctgcttgcc ggtctccact tcgggccaat cgaggaccgc
     7801 gacgtgcatc tggcgcggca ccgaattgcc atgcgcggaa ccggcgcggc cgtcaggctt
     7861 ctcgttgacg aatttgacgc catgctggag gaggaattcg aaggtcgggg cgcaattgtc
     7921 ggcaaaggcg cggatgatct cgcgatcgtt gaagcggtaa tcggcagcgc cattgggctc
     7981 ggtcagcgac cagtcggtca ggtcgcggaa cactagatcg ggactgtcct ggatgccggc
     8041 cttcttctgg atggacgtgc cgccgccgag cggaatattc gcgccggagc agatcgcgtg
     8101 gccgccaacg tccggttcgg cttcgatgat gataaccgag gagcccgcct cgcgcgcgac
     8161 gatggccgcg ggcatgccgg tggcgcccgc cccgatgatg acgacatcgg cttcgcggtc
     8221 ccagcgcgtg ggaagcgctt gggcctgggc cgccgccggt gtcgcggcaa ggccaccggc
     8281 cgcggcggcc gcgccggcaa gcgtgccggt cttgatcaga tcgcgccgcg agatgttgtt
     8341 cttgatggcc atggaaatcc tcccagatcg tttgacacga ctataccggc tcgatcgacg
     8401 gactgcaccg aatcttcctg ccttttgggg taatgccctg cctttggata atgcattgta
     8461 agcgtgcgac agcccgtggc atcctgacgc gaaggccatc ggctcaggag gcgagcatga
     8521 gattgtgtac cgtaatggcc gccgcgacat tcgcgacgtc cagcgcctat gcagcggcga
     8581 catatgaggg aagtggccgg gtgcccgagc agttcgtcct ttccggcaag gcggccgaac
     8641 gtctccacga ttatgtttcc atcaatctcg ataccgccga gaaattggtc gcggcctgcc
     8701 aagccagcgc gcgaaagatc aacagtccgg tggtcgtcgt tgtcctcgac ccgcaaggtc
     8761 tgccggttct ggaaagtcgc atggacggtg aaggctggat acagatcaag gcggcggaac
     8821 agaaaacatt gacggcgctg cgcacacgcg ccccttcgca tgtgctgacc aaccgcaacg
     8881 tccaaaatcc gttcacgagt cagaacgttg cgggctatgg gctcaccgcg aacaagggtg
     8941 gtcttcccat catcgtcaat ggccaactga ttggcgccat gggagcgggc ggaatggcga
     9001 ccccctccgg cgaggaatca tgcgtccgcg acgcgctcga gcaggtcatt ggtccgcagc
     9061 cgccattgct ggccgatgtg acgccccaac gcaacaacgc tcgctgaagg agaacgcccg
     9121 atggccttga ggtcatcgcc cgaggcaccg ccttggagcg tctggtggcg cgggggtacg
     9181 gccctcgctt atcatccggc tctttactgc gctctgcggc gcgcgcaatc tcccgtcctg
     9241 acaatgccgg cacgataatg tcagtatgat aaccgcggag gacatcgatg attgatcgga
     9301 tcagacgcaa catcctggca acgggtgccg cggtgaccgc aatggccgcg gcgccgcgcg
     9361 cattttctca gccggccggg caggcaggcg ccttttacga aaaaggcccc gttcgcatct
     9421 actatgagca ggagggttcc ggcttcccgt tattgctcat tgccggcgga ggattgaact
     9481 cgacgatctc cggtttgcgc cgtggcgatc cgttcaatcc tttcgaggag ttcaaggcag
     9541 actaccactg tgtcgcggcg gacctgcgca acgccaatgc cggccagtcc tcgggaccgc
     9601 ttgagatcga ccggccatgg gattcccata ccgacgatca tctcggcctg atggatcatc
     9661 tgggcattca gaaattcatg gtgatgggct tctgcattgg tggcccgttg atctggaatc
     9721 ttttgaagcg ggcgccggat cgcgtggtcg cagccgtgct ggcccagccc agcggattcc
     9781 ggccggagat gcccaccctc acctatgacg gcaatatggc cggatggggc cccgacctgg
     9841 tcaagcgccg gcccgacatc accatggata cggtccaaaa attcctgacc aggatgtacc
     9901 gcaacaaccc cgacttcgtg ataaccgtga cgcgggattt cgtgcgcaat tgccagacac
     9961 ccgtactgat cctccccgac gatatcccgg cgcatcccta tgccgtcgcc atggaggccg
    10021 cgatgctcgc accgaaagcc gaagtgagta tgttcccgtg gaagcagccc aaggagcgga
    10081 ttccgctggc ggtgcgccag attcgttctt tcctgaaggc ccaccggccc gtttccgttt
    10141 agggggctcg tccgtttagg aggctacgcc ctctagaact gtggggattt ttcttccggc
    10201 aagggtccga aactctggcc gttgaaggtc acattgttct cttcgcagat gacctcttcc
    10261 atggtctggg agcggttcac ccgataggtc agtctgccgg cccagggccc ggtgaaagtg
    10321 ccggggtcct cgacccggat ggtcacgtcc agacccttgc ccttttcatc cttgatcggc
    10381 cggtagcgct ccacgacatg cagctttgcg gtgtgcggcg tgccgaacgg atcgacgaac
    10441 gacatcgcgt gggttttcac gccgatggtg tcgatgacga gcgtgtcgcc ttcgtaatgg
    10501 ccgaccgatt ctccgtacca ggacggttcc accttcgcgg aatgtttctg attcaaccag
    10561 acgcggcgga tctgatggtc gcgctgatac aggatcgtga cgcggtcctt gttttgcagg
    10621 aactgcacag cctcgcgcat gttcaggatg gcgggcaccg tcaccggcca gcaggaatcg
    10681 tcggcctgat agacatgctt caaggcgact tcgctgtcgg cgttcttctt gacgatggcg
    10741 cgcgcccagg gttgcaggat gggattgtcc caattgccgg ccatgatctc ttcggcgtcc
    10801 ttgctgttca cctcgagccg ggcgatgggc ttgccctcct tggcgccggg gatgggctcg
    10861 aacatgggtt cgccggtgcg cacccagagg ccggacaaat caggcgcgcc ctcgtccgag
    10921 gcccccacgt tcgcggcgag aatcggaccg gcggacgcgg acatcagcac cgccgcaacc
    10981 atcgcgctca gcgcaaaccc tgtcttcgac attgggcaac ccctgtttcg gttgtccgca
    11041 ctatatcggg tccgataggc tgaggaaaac catggaaatg gcccgcattc tgggctcttg
    11101 gtaggcaatc gacgccatgc cacagtcgcg gccgattttt tccggtacgc gctgccatga
    11161 tggaacaaaa gaataccgct ccccgcatcg cctatggcga tttgcgcgaa tggctggcgc
    11221 tggcggaccg gttgggcgag gtgcgccatg tggcgggcgc gagctggcag gaggatatcg
    11281 gtcttgccgc cgaagcgatc ctgcgcgccg agaacggccc ctgcgtcctg ttcgacgatg
    11341 tgccgggctg tgccaagggc ttccgcctgc tgctcaacat gttcgccggc acacggcgca
    11401 acatgacgct cggctttccc tatttgctga cgaaatggga attgtccgat gcctatcggg
    11461 cggcctatct cgccaatccg aagcttgtgc cgcatgaaat cgtcgccgat gggccggtgc
    11521 tcgaaaatgt gctgaccggc gatgcggtcg atgtgctgaa attccccgct cccgtctggc
    11581 acgaaaaaga cggcggccgc tacatcggca ccggcacgtt ctccatcacg cgcgatccgg
    11641 aggaaggctg gctgaacgcc ggggcctatc gcgcgcaggt gcatgatgcg aaatcggtgg
    11701 gcctcgtcat ggcggcgggc catcatggcg ggcagcatta cgaaaaatat caaaaacgcg
    11761 gcgagcccat gccggtcgcc atggtgctgg gcggcgatcc gctgtcgttt ttctacggcg
    11821 ggctggaagt gccctatggc acgttcgaac tcgacgtagt cggcggcttg cgcgggcggc
    11881 ccgtgaagat ggtgaggggc cgcatcacag gcctgccctt cccggcggcg gccgaaatcg
    11941 tgctcgaagg ctatgtgcag ccggataaga cgcggatcga gggcccgttt ggcgaatgga
    12001 ccggccacta cgcgggcggc gccaaacctt ggccggtgct ggacgtacac gcgatctatc
    12061 accgcaacga tccgatcctt gtcggcgtgc cgccgatggg cgcgggtccg gacgagatgg
    12121 cgcgctatcg cgccgtcatg cgttcggcca ccatcaagga gaacatgatc cgcgccggag
    12181 tgccgggcgt ggaacaggtc tggtgccatg aggtgggggc cgcgcgctcg ttccacggcg
    12241 tctccatcaa acagcgttat ccgggccacg ccgtgcaggc cggaacgatt gccgcgcaat
    12301 gcggcggctc ggcctatgcg tcgaaatata tcgtggtgac ggatgaggat gtcgacgtca
    12361 ccaatct
//
LOCUS       DASYEG010000043         7587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4941817, whole genome shotgun sequence.
ACCESSION   DASYEG010000043 DASYEG010000000
VERSION     DASYEG010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 7587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7587
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4941817"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..2218
                     /gene="acs"
                     /locus_tag="VGM72_01650"
     CDS             281..2218
                     /gene="acs"
                     /locus_tag="VGM72_01650"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY4263995.1"
                     /translation="MSDTLIPVPDAWKSRAYLNAERYDDWYAESIADPESFWGREGKT
                     LAWIKPFTSVKDTSFDLHDFRISWFADGTLNVSANCIDRHLPARSHQTAIIWEGDDPA
                     QSRHITYAELHREVCRFANVLKANGAKRGDRITIYLPMIPEAAYAMLACARIGAIHSV
                     VFAGFSPDSLAGRILDCQSQIVITADEGLRGGKAVPLKRNTDVALTQCPDVQRVIVVK
                     RTGAKEMMHAGRDVWYHDEAAKVSDQCAPVEMGAEDPLFILYTSGSTGKPKGVLHTSA
                     GYLCYVAFTHKYVFDYHDGDVYWCTADVGWVTGHSYIVYGPLANGATTLMFEGIPNHP
                     STSRFWEVIDKHKVNIFYTAPTAIRALMREGEKPVKQTSRASLRLLGSVGEPINPEAW
                     LWYYRVVGDERCPIVDTWWQTETGGILISPLPGATDLKPGSATKPLFGIRPALVDQGG
                     NILDGAATGNLVLTDSWPGQMRSVYGDHQRFIDTYFKAYPGMYFTGDGCRRDEDGYYW
                     ITGRVDDVINVSGHRLGTAEVESALVSDLRVAEAAVVGYPHDIKGQGIYAYVTLKLGQ
                     SPSEALEAELKQWVGAQISPIARPDVIQFAPGLPKTRSGKIMRRILRKIAENDIAHVG
                     DISTLADPSVVDDLVKNRRSG"
     gene            complement(2258..2542)
                     /locus_tag="VGM72_01655"
     CDS             complement(2258..2542)
                     /locus_tag="VGM72_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4263996.1"
                     /translation="MGSPAPEHGGTVRVFVTAKSEELMMAEIRETHIVKEREGSSATP
                     WLAFIVGALLIAVVAVFLLNAHGRVSGPAGSVDLNIKSPVTLPANPAPAK"
     gene            complement(2563..2853)
                     /locus_tag="VGM72_01660"
     CDS             complement(2563..2853)
                     /locus_tag="VGM72_01660"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY4263997.1"
                     /translation="MSKLEGDARKQALAELTGWREVAGRDAISKIFIFKDFNQAFGFM
                     TRAALAAEKMDHHPEWSNVYKTVEVTLSAHDAGGVTVKDIALAKAMDRFAGI"
     gene            2919..3332
                     /locus_tag="VGM72_01665"
     CDS             2919..3332
                     /locus_tag="VGM72_01665"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEY4263998.1"
                     /translation="MDWTRIEAPSLDDIETLARDAFASLPEGFRALTGEIVFMVQDFP
                     DEDVIEEMGLETGFDILGLFQGADLASREGASSSHSPTMIFLYRRPILDYWAEHGEPL
                     GHIVRHVLIHEIGHHFGLSDDGMDAIETRENHPSL"
     gene            complement(3317..3850)
                     /gene="thpR"
                     /locus_tag="VGM72_01670"
     CDS             complement(3317..3850)
                     /gene="thpR"
                     /locus_tag="VGM72_01670"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEY4263999.1"
                     /translation="MIRLFVALELPETVRDRLTALEGGVPGARWAGDEQLHLTLRFIG
                     EVDENVANDIDDALAGIRTPAFALELAGVGEFGGKKPRALWAGVRANDALLHLQRKIE
                     TALQRIGLPAEERKFSPHVTLARMHSAPQEKIVQFLTQHALFASGPFPVDRFVLFSSQ
                     LGSGGSVYHAERIYKLG"
     gene            complement(3847..5376)
                     /gene="murJ"
                     /locus_tag="VGM72_01675"
     CDS             complement(3847..5376)
                     /gene="murJ"
                     /locus_tag="VGM72_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEY4264000.1"
                     /translation="MIGRLFSVGGFTLLSRITGFGRDIMMAAVLGSGPMSDAFLVALR
                     LPNSFRSIFGEGAFNAAFIQRFAKVKTQEGSKAAADFANRVFSWQMVAQVVLLVVALA
                     DMEPVVRVLAPGFVARPDQLALAVSLSRIAFPYLLMTVVAIQLSAMLNANGKFAAAAA
                     WSLLLNVAMMATLGAARWFPNPAYAAAWGVFLAGLLQLVFIVPTAARHGLGLRINAPR
                     WTPDMREFLRALGAATFGSASVQILLFVDTLIASFLPAGALTALYYSDRINQLPMGTL
                     GIALGTVLLPEMSTRIAQGDAGAANAAQNRAAALGLLFTLPFVAAYFAVPLTIMRGLF
                     AHGAFHLEAAELSAAALMAYGVGLPAFVLIRVVAPSFYARGDTGSPVRATMISVLVNI
                     ALKFAFVWGLGFGAVGLALGTSLAIWVNVAVLIAMARRRKLIAVDRVFWNALGPSVVA
                     AAAAGAGALAGVRLADSVTRSAWGDVIALIFAAVLGGLAYGAVVFLFRRRLPLGRWAQ
                     P"
     gene            complement(5412..6086)
                     /locus_tag="VGM72_01680"
     CDS             complement(5412..6086)
                     /locus_tag="VGM72_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEY4264001.1"
                     /translation="MGLFFKTILSALVLSLLLARGVEAAEHNIRILAFGDSLTQGYGV
                     PPGMDFPARLQQALTQKGLKVAVVNAGVSGDTSAGGLARLDWSLGDAKDAPDAAIVEL
                     GANDGLRGLAPAEMEKNLDGILAKLQQRKIPVLLAGMKSPRNLGASYAAEYDAVFPRL
                     AKKYGVLFYPFFLEGVALKADLIQPDGLHPNPKGVDAIVKNILPLARKLVQQAQAASI
                     APGSAR"
     gene            6110..6835
                     /locus_tag="VGM72_01685"
     CDS             6110..6835
                     /locus_tag="VGM72_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4264002.1"
                     /translation="MRASATRDTPPTTARAPSILLEDVRLTLASAAGAVNILNGVSVA
                     VEAGEAVALLGPSGSGKSSLLMVAAGLETPTAGRVGVAGRTITGLGEDALARFRREHV
                     GIVFQSFHLVPTMSALENVAVPLELLRRANAFESARAALEAVGLAHRLDHYPGQLSGG
                     EQQRVALARAIVHEPDVLFADEPTGNLDGATGAGIVDLLFALRARVNATLFLVTHEEA
                     LAARCDRTLRMKDGLIVAEERTK"
     gene            6832..>7587
                     /locus_tag="VGM72_01690"
     CDS             6832..>7587
                     /locus_tag="VGM72_01690"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4264003.1"
                     /translation="MSAFALAIRFARRELRSGLSGFRIFLAALTLGVAAIAGIGSLGE
                     TFLTGLSEQGRTLLGGDVRLQRLYQPATDAERAFLVQYGAVTESAALRSMATNANQPD
                     RRTLIELKAVDGAYPLLGETVLEPAMALQTALACDASTCGAVAEEALAARLGLRIGDA
                     IRIGNADFTLRARIVSEPDRVAGGFALGPHVLVSRDGLRRAGLVTEGSLINYAYRVAF
                     ARDATPELFHANLEAAFPKASWEFNDRSNALPQV"
BASE COUNT         1515 a         2450 c         2317 g         1305 t
ORIGIN      
        1 atgtgctcga gataattgcg cccgacgcaa tagacgcgcc ggaccggaaa tcgctggccg
       61 ccgcccacga tgggaagcga aaccaccgga atcgaaaaca ccatgccgga attggaatcg
      121 gacgccatgc cttcaaatct cccctacgca ttgtcgaacc acacgccttg tagcattgct
      181 ggaagataac aaaaggaaat ccgtcccgcc gtcgcgatca agcggcttgt acggcaaacc
      241 tcgccccgtc aatatcgcgc caaacaatcg aggaaaccgc atgtccgaca cgctgatccc
      301 cgttcccgac gcgtggaaat cacgcgctta cctcaatgcc gagcgatatg acgattggta
      361 tgccgagtcc atcgccgacc ccgagagctt ctggggcaga gagggcaaga cgctcgcctg
      421 gatcaagccc ttcacgagcg tgaaggacac ctcgttcgat ctgcacgatt ttcgtataag
      481 ctggttcgcg gatggcacgc tgaacgtctc ggccaactgc atcgaccggc atctgccggc
      541 ccgcagccac cagaccgcca tcatctggga gggcgacgat ccggctcaat cgcgtcatat
      601 cacctatgcc gaattacatc gcgaggtctg ccgctttgcc aatgtgctga aggcgaacgg
      661 cgccaaacgc ggcgaccgga tcacgatcta tctgccgatg atccccgaag ccgcctacgc
      721 catgctcgcc tgcgcgcgca tcggagcaat ccattccgtg gttttcgccg gcttctctcc
      781 ggacagcctc gcggggcgca tcctggactg ccagagccag atcgtgatta cggccgatga
      841 aggcctgcgc ggcggcaagg cggtgccgct caaacgcaac accgatgtcg cgctaaccca
      901 atgcccggat gtccaacgcg tcatcgtggt caagcggacc ggtgccaagg aaatgatgca
      961 tgcgggccgc gacgtctggt atcacgacga agccgcgaag gtttcagacc aatgcgctcc
     1021 cgtcgagatg ggcgcggagg acccgctgtt catcctctat acctccggtt cgacgggcaa
     1081 accgaagggc gtgctgcaca ccagcgccgg ttatctctgt tacgtcgcat tcacccacaa
     1141 atacgtcttc gattaccacg atggcgatgt ctattggtgc acggccgatg tcggctgggt
     1201 gacgggtcac agctacatcg tctatgggcc gctcgccaac ggcgccacca cgctgatgtt
     1261 cgaaggcatt ccgaaccatc ccagcacgtc gcgtttctgg gaggtgatcg acaagcacaa
     1321 ggtcaatatc ttctacaccg cgccgaccgc catccgcgct ttgatgcgcg agggcgagaa
     1381 accggtgaag cagaccagcc gcgcgtcttt gcggcttctg ggctcggtgg gagagccgat
     1441 caatccggaa gcctggctct ggtattaccg tgtggtggga gatgagcgct gccccattgt
     1501 cgacacctgg tggcagacgg aaacgggcgg gattttgatc tcgccgctgc ccggcgcgac
     1561 cgatctgaag ccgggctccg cgaccaagcc gctgttcggg atcaggcccg cccttgtgga
     1621 ccagggcggg aacattctcg acggtgccgc gaccggcaat ctcgtgctca ccgattcctg
     1681 gccaggccag atgcgcagcg tctatggcga tcaccagcgc ttcatcgaca cttatttcaa
     1741 agcctatccg ggcatgtact ttaccggcga tggctgccgc cgcgacgagg acggttatta
     1801 ctggatcacg ggacgcgtcg atgatgtaat caacgtgtcg ggccacaggc tgggcacggc
     1861 ggaggtggaa tccgcgctgg tgtccgatct gcgcgtcgcc gaagccgccg tggtcggcta
     1921 cccgcacgac atcaagggcc agggcatcta cgcctatgtg acgctgaagc tcggacaatc
     1981 gccgtcagag gccctggagg cggagttgaa gcaatgggtg ggggcgcaga tcagccccat
     2041 cgcgcggccc gacgtgatcc agttcgcacc gggccttccc aagacacgct cgggcaagat
     2101 catgcggcgt atcctgcgca agatcgcgga gaacgacatc gcccatgtgg gcgatatctc
     2161 aacgctcgcc gatccttcgg tggtggacga tctggtgaag aaccgacgga gtggatgaat
     2221 agataagtcc ggctcgacta cgccgggccg gacttatcta tttcgcggga gcagggttcg
     2281 ccggcaaagt gaccggcgac ttgatattca aatccacgct gcccgcgggc ccgctgaccc
     2341 tgccgtgcgc attcaacaga aataccgcaa ccaccgcgat cagcaacgcc ccgacaatga
     2401 aggcgagcca cggcgtcgcg ctgcttccct cgcgctcctt gacgatatgt gtttcccgaa
     2461 tttcggccat catcagctcc tcacttttcg ctgtaacgaa aacgcgaact gtgccgccat
     2521 gttccggtgc gggcgaaccc acggccaagc tccagcagcc ggtcaaatcc ctgcgaaacg
     2581 gtccatcgcc ttggcgagcg cgatatcctt gaccgtgacg ccgccggcat catgggcgga
     2641 aagggtgacc tcgacggttt tgtagacatt cgaccattct gggtgatggt ccatcttttc
     2701 ggcggccagc gccgcgcgcg tcatgaatcc aaaggcctgg ttgaaatcct tgaaaatgaa
     2761 aatctttgaa atcgcgtcgc ggcccgcgac ttcgcgccag ccggtcagtt ccgcgagcgc
     2821 ttgcttgcgt gcatcgcctt cgagcttgga catcgcctac ctcatgtcat gaatgttgct
     2881 agtttgcgcc gacacctgca tcatgccaat cctaaaccat ggactggaca cgcatcgaag
     2941 ctcccagcct cgacgacatc gagacgcttg cgcgtgacgc cttcgcgagc ctgcccgagg
     3001 gttttcgtgc gctgaccggc gaaatcgtct tcatggtcca ggatttcccc gatgaagacg
     3061 tgatcgaaga gatgggattg gagaccggct tcgatattct cggtctgttc cagggggccg
     3121 atctggcatc gcgcgaaggt gcgtcgtcct cgcacagtcc cacgatgatt ttcctctatc
     3181 gccgtccgat cctggattat tgggccgagc acggagaacc gctcggccat attgtccgcc
     3241 atgtgctgat ccacgagatc gggcatcatt tcggcctctc cgacgacggt atggacgcga
     3301 tcgagacccg cgaaaatcac ccgagcttgt aaatccgctc cgcgtgatag accgagccgc
     3361 cgctgccgag ctggcttgaa aacaacacga aacgatcgac tgggaatggt ccgctcgcga
     3421 acagcgcatg ttgggtcagg aactgcacga tcttctcttg cggggccgag tgcatgcggg
     3481 ccagcgtcac atggggcgag aatttgcgct cttccgcagg caggccgatg cgttgcagag
     3541 cggtttcgat cttcctctgc agatgcagga gcgcatcgtt cgcgcgtacc ccggcccaca
     3601 gcgcgcgcgg tttcttgccg ccgaattcgc ccacacccgc aagctcaagc gcaaaggccg
     3661 gggtgcggat gccggccaaa gcatcgtcga tgtcgttggc gacattttca tcgacttcgc
     3721 cgatgaaacg cagagtcaaa tgaagctgct catcgcccgc ccagcgtgcg ccgggcacac
     3781 cgccttcaag cgcggtcaag cggtcgcgca ccgtttcggg cagttccaat gcgacgaaca
     3841 aacggatcat ggctgcgccc atctccccag cggcaagcgg cggcgaaaaa gaaatacgac
     3901 cgcgccataa gccaatccac ccagcacggc cgcgaatatc aacgcaatca cgtcacccca
     3961 tgcgctccgc gtcacgctgt cggcgagccg cacgccggca agtgcgcccg cgcccgccgc
     4021 cgccgccgcg acgacgctcg gtcccaacgc gttccagaac acgcggtcga cggcaatgag
     4081 tttgcggcgt ctggccatgg cgatcaacac tgcgacattc acccagatcg ccagcgaagt
     4141 gccgagcgcg aggccaaccg caccgaagcc aagcccccag acgaacgcga atttcagcgc
     4201 gatgttgacg agcaccgaaa tcatcgtggc ccgcaccgga ctgccggtgt cgccgcgcgc
     4261 ataaaaactg ggtgcgacca cacggatgag gacgaaggcc ggcagaccga cgccataagc
     4321 catcagcgcg gcggcggaca attcggcggc ttcgagatga aacgccccat gcgcgaaaag
     4381 cccgcgcatg atcgtcagag gcacggcgaa ataggcagcg acaaagggca gcgtgaacag
     4441 caatccgagc gcggcggcgc ggttttgcgc cgcgtttgca gcccccgcat cgccttgcgc
     4501 gatgcgcgtc gacatttcag gcagcagcac tgtaccgagc gcgatgccga gcgtgcccat
     4561 cggcaattgg ttgatgcggt cggaataata gagcgccgtc agtgcgccgg ccggcaggaa
     4621 actcgcgatc agcgtatcga cgaacaacag gatttgcacg ctggcagaac caaacgtcgc
     4681 cgcccccagc gcccgcagga attcgcgcat atccggggtc cagcgaggcg cgttgatcct
     4741 gagcccgagg ccgtggcgcg cggccgtcgg cacgatgaag acaagctgca acaggccggc
     4801 aaggaacacg ccccaggccg cggcataggc cggattggga aaccagcgtg ccgcgcccag
     4861 cgtcgccatc atcgcgacat tgagcagaag cgaccaggca gcggcggcag caaacttgcc
     4921 attggcattc agcatcgcgg agagctggat cgccaccacc gtcatgagca gatagggaaa
     4981 ggcgatgcgc gaaagactga ccgcgagggc gagttgatcg gggcgcgcca cgaaacccgg
     5041 cgccagcacg cgcaccaccg gttccatgtc ggcgagtgcg accaccagca gcacgacctg
     5101 cgccaccatc tgccaggaaa atacgcgatt ggcgaaatcc gcggccgcct tcgatccctc
     5161 ttgcgttttc accttggcga agcgttggat aaaagcggca ttgaacgcgc cttcgccgaa
     5221 gatcgagcga aaactgttgg gcaggcgcag cgcgaccagg aaagcgtcgg acatcggccc
     5281 cgaacccaga accgccgcca tcatgatgtc gcgcccgaag ccggtgatgc gcgacaaaag
     5341 cgtgaagcca ccaacggaaa acagccggcc gatcacgctt gcgggtcttt cgctctgcga
     5401 atcatccccg attatcgcgc ggatcctggc gcaatggagg ctgcttgtgc ctgctgaacc
     5461 agcttccggg ccagcggcag aatattcttc acgatcgcat cgacgccctt cggattgggg
     5521 tgcagcccgt cgggctggat caggtccgcc ttgagcgcaa cgccttccag gaagaaggga
     5581 tagaacagga caccgtattt tttggccagg cgcggaaaga cggcatcata ttccgcagca
     5641 taggacgcac cgagattgcg cggacttttc atgccggcca gcagcacggg gattttgcgt
     5701 tgctgaagtt tggcgagaat cccgtccaga tttttttcca tctcggccgg ggcaagcccg
     5761 cgcaacccgt cattggcacc gagttcgaca atcgccgcgt cgggcgcgtc cttcgcatcg
     5821 ccgagcgacc aatccagacg cgccagcccg cccgcgctgg tgtcgcccga aacccccgca
     5881 ttgacgacag cgactttcag tcccttttgc gtgagcgcct gttgcagccg cgccggaaaa
     5941 tccatgcccg gaggaacgcc atagccctgg gtgagactgt cgccaaatgc cagaatgcga
     6001 atgttgtgct cggcggcctc aacaccccgg gcaagcagca acgacaacac caacgccgaa
     6061 agaattgtct tgaagaaaag gcccatcgtc actatcgcct gatatcgcca tgcgagcatc
     6121 cgccacacgc gacacgccgc ccaccaccgc gcgcgcgcca tccatcctgc tcgaagatgt
     6181 aaggctgaca ttggcgagcg cggccggggc ggtcaacatc ctcaatggcg tctcggtcgc
     6241 ggtggaggcc ggtgaggcgg ttgctctgct tggcccctcg ggttcgggaa aatcctcgct
     6301 gctgatggtg gcggcgggac tggaaacccc gacggcgggg cgcgtcggcg tcgcgggacg
     6361 cacgatcacc gggctcggcg aagacgcgct cgcgcggttc cggcgcgaac atgtcgggat
     6421 cgtcttccag tcctttcatc tggtgccgac catgagcgcg ctcgagaacg tggcggttcc
     6481 gctcgaactg ctgcggcgcg caaatgcatt cgaatccgcg cgggcggcgc ttgaggcggt
     6541 gggcctcgcc catcggctgg accattatcc cggacaattg tcgggcggcg aacagcagcg
     6601 cgtggcgctg gcgcgcgcca tcgtgcatga gcccgacgtg ctgttcgccg atgagccgac
     6661 cggcaatctc gacggcgcca cgggcgcagg catcgtcgat ctgttgttcg cgctgcgcgc
     6721 gcgcgtcaac gcaacccttt tcctggtcac ccatgaagaa gccctggcgg cacgctgcga
     6781 ccggactttg cgcatgaagg acggcctgat tgtggcggag gaacggacaa aatgagtgcg
     6841 tttgcgctgg cgatccgttt tgcgcggcgc gaactcagaa gcggcctctc gggttttcgc
     6901 atcttcctgg cggctttgac gctcggagtt gccgcgatcg ccggcatcgg atctctgggg
     6961 gaaaccttcc tgacgggact gtccgaacag ggccgcacac tcctcggcgg cgatgtgcgg
     7021 ctgcagcggc tctaccaacc cgccacagat gcggaacgcg cgttcttggt ccaatatggg
     7081 gccgtcaccg aatccgcagc cctccgcagc atggcgacca acgccaatca gccggacagg
     7141 cgcacgctga tcgaattgaa ggccgtggac ggtgcctatc cgctgttggg ggagacggtg
     7201 ctcgaaccgg cgatggcgct acagacggca ctcgcctgcg atgcaagcac ttgcggcgcg
     7261 gtggccgagg aggcgctcgc cgcccggctc ggcttgagaa tcggcgacgc catccgcatc
     7321 ggcaacgcgg atttcacctt gcgcgcgcgc atcgtgtcgg agcccgaccg cgtcgcaggc
     7381 ggatttgcgc tggggcccca cgttctggtc tcccgcgacg ggctcaggcg cgccggcctg
     7441 gtcaccgaag gcagcctcat caattatgcc taccgcgtgg cattcgcgcg cgatgccacg
     7501 cccgaactgt tccacgcgaa tctggaagcc gcctttccca aagcctcctg ggagttcaac
     7561 gatcgcagca acgcgctgcc gcaggtg
//
LOCUS       DASYEG010000044         4396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1319502, whole genome shotgun sequence.
ACCESSION   DASYEG010000044 DASYEG010000000
VERSION     DASYEG010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319502"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VGM72_01695"
     CDS             <1..573
                     /locus_tag="VGM72_01695"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEY4264004.1"
                     /translation="LDYGAGKLNLISPNHCEGKVVYWQAPTVAIVPMRVDTQGKITFP
                     LTLDGHTIRAILDTGATRTALNLDVAKRVFNIDTSDPDLQKVGELKGGYTAQIYNRKF
                     KSLSIEGVTVTDPLITLLPNMVSLTSEQRSAGSLVRDTDKQAPDMLLGMSVLNKLHVY
                     IAAHEGKLYITQASTPAPNPEPLPNISAAQ"
     gene            672..2021
                     /locus_tag="VGM72_01700"
     CDS             672..2021
                     /locus_tag="VGM72_01700"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY4264005.1"
                     /translation="MSTLPDPTAADRLDRLVKGALKSGADAADAVEFKNISVAVSYRL
                     GKLEDVGRAESSDLGLRVFIGRQVAFVSSTDFSENALSALPERAVAMARLAPEDKYAG
                     LAPRERLAQAIPDVEIDDPQEPSTVLLVERARAAEGAALEVPGVTNSEGGSADYGRTM
                     VTLATSEGFLGGYVGTSRSVAVSVIAGEGTEMERDYDYAQTRFASDLESAEAVGKRAA
                     MRAVKRLNPRKVKTQSVPVIFDPRVASSLLGHFAGAISGAAIARGVSFLKDRMGEQVF
                     GENITIIDDPHRKRGTRSKPFDGEGVANRMTVLIDKGRLTTWLLDSASARQLGLQTTG
                     HAARGTGGPPSPSTTNLHMAAGALSPVELMADIREGFYVTDLIGMGVNGVTGDYSRGA
                     AGFWIENGEIAYPVSEVTIAGNLKDMYRNLTPADDLVFRYGTDSPTLRVEGLTIAGE"
     gene            2008..2802
                     /locus_tag="VGM72_01705"
     CDS             2008..2802
                     /locus_tag="VGM72_01705"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEY4264006.1"
                     /translation="MPENDLADTGLLVAAVREAGTIARHFYGGTYKSWQKSKGNPVTE
                     ADIAIDDFLKKTLLAARPDYGWLSEESIDDPARLARARIFVVDPIDGTYGFLKQRPHF
                     TIVATVVEKGRPVSGAIYNPITEEMYEAAYGAGAQLNGKPIHVSGQSDFPHMRLLAEK
                     AVTDSTRWTVPWPASIVAETRASAAYRMALVAAGEFDAMISLSRKADWDIAAGDLIVT
                     EAGGRVTTRSGDTQVYNQANPVHRNIICAGPALHARLIERMRDLRE"
     gene            2880..>4396
                     /locus_tag="VGM72_01710"
     CDS             2880..>4396
                     /locus_tag="VGM72_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4264007.1"
                     /translation="MKTNPSAAERPVAQGDWPLIRRLLRDYLGPQKTVLAGAILCMIG
                     GALTPPAQAWLVGPAVNYLFLHPRADMLFLIPAALVGLVIVRAAANFGEAVLVGSLGQ
                     RITAGTQTDMAQSMVGFDLKTLNAVHSGAFISNFLYDATLLREAVVKGILACGKEIIS
                     LILFAALMLYQNWRLTLISLVVLPIIAVVTRNLGRSVRKASGQGMVDSGALSTALAEM
                     LDGRRIVKAYGLEEHTLARIQANIDRRLESLLKVLRSSAISAPAADFFGGLGLAVVLF
                     ISGYLGTHGQLTADQFASFLAAMLLAQQPVRNLSNVWTVTTTGIAAAKRVYEIVDTEP
                     SIKDAPGAKGLRISAPPFGGNVRFAGVSFAYQAGSAALDNISFEVPAGKKVALVGPSG
                     AGKSTIFNLLLRFYDANNGRIDIDGQDIHAVTLNSLRGHLALVTQEPFLFDDTIAANI
                     GYGRAGATQDEIESAARSAAAHEFIAALPMGYDTRTGEGGLRLSGGQRQRIAIARA"
BASE COUNT          807 a         1421 c         1334 g          834 t
ORIGIN      
        1 ctggattacg gcgccggcaa actcaatctg atttcgccca atcattgcga gggcaaggtc
       61 gtctattggc aggctccgac ggttgccatt gtgcctatgc gcgtggatac ccagggaaag
      121 atcacgtttc cgctgacatt ggacggacac accattcgag ccatcctcga cacaggggca
      181 acaaggaccg cgctcaatct cgacgtggcc aagcgcgtct tcaatatcga cacgagcgat
      241 cccgatctcc aaaaggtggg agaactcaaa ggcggctaca cagcacaaat ctacaatcga
      301 aaattcaaga gcctatcgat cgaaggtgtc accgtcaccg atcccctgat tacgctgctg
      361 ccgaacatgg tgagtttgac atccgaacaa cgttccgccg gttcgctggt ccgcgacacc
      421 gacaagcagg cacccgatat gctgctcggc atgtcggtcc tgaacaaact tcacgtctat
      481 atcgccgccc atgagggaaa actttacatc acgcaggcct cgacccctgc tcccaacccg
      541 gaaccgctgc ccaacatctc ggccgcgcaa taggcgcggg gcggccggcc gccccctctc
      601 acagctatga atccggcgcg cgcgcgttta tgagagacgc tcgcatatgc ttggctattg
      661 ccggagtttc catgagcacc ctgcccgacc ccaccgccgc cgaccgtctc gaccgcctgg
      721 tcaagggcgc gctcaaatcc ggcgccgacg cggccgatgc cgtcgagttc aagaacatct
      781 cggtcgctgt ctcctatcgg ctcggaaagc tcgaagatgt cggacgggcg gaatcctccg
      841 atctgggctt gcgcgttttc atcggccggc aggtggcgtt tgtttcctcg acggattttt
      901 cggaaaacgc gctttcggcg ctgcctgagc gagcggtcgc catggcccgg ctcgcgccgg
      961 aggacaaata tgccggactc gcgcctcggg aaagactggc gcaagccatt ccggatgtgg
     1021 agatcgacga ccctcaggag ccttcgaccg tgcttttggt ggaacgtgcc cgcgcggccg
     1081 agggcgccgc cctggaggtt ccgggcgtga ccaattcgga aggcggcagc gccgattacg
     1141 gccgcaccat ggtaacgctc gccaccagcg agggcttcct cggaggctat gtcggcacca
     1201 gccgcagcgt cgcggtgtcg gtcattgccg gcgaaggcac cgagatggag cgcgattatg
     1261 actacgcgca gacacgcttc gcctccgacc tggaatcggc cgaggcggtc ggcaagcgcg
     1321 ccgcgatgcg cgccgtcaaa cgcctcaatc cgcgcaaggt gaagacccaa tccgtgccgg
     1381 tcatcttcga cccgcgcgtg gcgtccagcc tgctcggtca ttttgccggt gcgatctcgg
     1441 gcgcagccat cgcccgcggc gtcagcttcc tgaaagaccg catgggcgag caggtgttcg
     1501 gggaaaacat caccatcatc gatgatcccc accgcaaacg gggcacgcgc tccaagccgt
     1561 tcgacggcga aggcgtcgcc aaccggatga cggtcctgat cgacaagggc cggctcacca
     1621 cctggctttt ggattcggct tccgcccgcc aattgggcct tcagaccacc ggccatgccg
     1681 cgcgcggcac gggcggaccg ccctcgccct cgaccaccaa tctccacatg gccgccggcg
     1741 cgctttctcc cgtcgagttg atggccgata tccgcgaagg cttctacgtc accgatctga
     1801 tcggcatggg cgtgaacggc gtcaccggcg attattcgcg gggtgcggcg ggtttctgga
     1861 tcgagaatgg cgagatcgcc tatccggtca gcgaagtcac catcgcgggc aatctgaaag
     1921 atatgtaccg caatttgacg ccggccgacg atctggtttt ccgctatggc acggattcgc
     1981 ccacgctgcg cgtcgaagga ctgacgattg ccggagaatg atctcgccga taccggtctt
     2041 ctggttgcgg ccgtccggga ggcgggaaca atcgcgcggc acttctatgg cggcacctac
     2101 aaaagctggc agaagagcaa aggcaatccc gtcaccgaag ccgatatcgc gatcgacgat
     2161 ttcctgaaga agacgcttct tgccgctcgc cccgattatg gctggctctc ggaagaatct
     2221 attgacgatc cggcgcggct cgcgcgcgca cgcatattcg tggtcgatcc catcgacggc
     2281 acctatggat ttctgaagca gcggccgcat ttcaccatcg tggctacagt ggtggaaaaa
     2341 ggccggcccg tgtcgggcgc catctataat ccgatcacgg aggaaatgta cgaggccgcg
     2401 tatggcgcag gtgcgcagct gaacggcaaa cctatccatg tcagcggcca aagcgatttt
     2461 ccgcatatgc gattgcttgc ggagaaagcc gtcaccgatt ccacgcgctg gaccgtgccc
     2521 tggcccgctt cgattgtggc cgaaacgcgc gcctccgccg cctaccgcat ggcgctcgtg
     2581 gccgccgggg aattcgacgc catgatttcg ctcagccgca aggcggattg ggacatcgcc
     2641 gcgggcgacc tcatcgtgac ggaggccggg ggccgcgtca ccacgcgcag cggagatacg
     2701 caggtctata atcaggctaa tccggtgcat cgcaacatca tctgcgccgg gccggccctt
     2761 catgcgcgcc tcatcgaacg catgcgggat ttgcgcgaat agcgccattt cctgcccggc
     2821 ctgggcatcc caagcgatgg agggtgaatg cgccaccccg cgcatgctaa gagcctctca
     2881 tgaagacgaa tccttccgca gccgaaaggc ccgtggccca gggcgactgg ccgttgatcc
     2941 gccggctgct gcgcgattat ctcgggccgc aaaagacggt tctcgcgggc gccattcttt
     3001 gcatgatcgg cggcgcgctc acccctccgg cgcaggcctg gctggtcggt ccggcggtga
     3061 actatctttt cctgcatccg cgcgccgaca tgctctttct catcccggcg gcactggttg
     3121 gcctcgtgat cgtgcgcgcg gcggccaatt tcggagaagc ggttctggtc ggctcgctcg
     3181 gccagcgcat caccgcggga acccagaccg atatggccca gagcatggtc ggattcgatc
     3241 tgaagacgct gaatgccgtg cattcgggtg ccttcatctc gaattttctc tatgacgcca
     3301 cgctcttgcg cgaggccgtc gtcaaaggga ttctcgcctg cggcaaagag atcatctccc
     3361 tcatcctgtt cgcggcgctg atgctctatc agaattggcg gctgacgctg atttcgctcg
     3421 tcgtgctgcc catcatcgca gtggtcacac gcaatctcgg ccgcagcgtg cgcaaggcct
     3481 ccggtcaggg catggtggac agcggggcgc tgtccaccgc ccttgccgaa atgcttgacg
     3541 gacgacggat cgtcaaagcc tatgggctgg aggaacacac actcgcccgc attcaagcca
     3601 atatcgatcg tcgcctggag tcgctgttga aggtgttgcg gtcgagcgcg atttccgcgc
     3661 ccgccgcgga tttcttcggt gggctcggcc tcgccgtcgt gctttttatc tcaggctatc
     3721 tcggcacgca cgggcagctt acggccgatc aattcgcctc ttttctggcc gcgatgcttc
     3781 tggcccaaca gccggtgcgc aatctatcga atgtgtggac ggtgacgaca accggcattg
     3841 cggcggcgaa gcgcgtttat gagatcgtcg acaccgagcc gtccatcaag gatgcgccgg
     3901 gcgccaaggg cttgcgcatt tccgcgccgc ccttcggcgg caatgtgcgc tttgccggcg
     3961 tcagcttcgc ctatcaggcg ggttcggcgg cgctggacaa tatttcgttc gaggtcccgg
     4021 ccggcaagaa agtcgcgctg gttggcccgt caggcgctgg aaaaagcacg atcttcaatc
     4081 tgctgctcag gttttacgac gcgaacaacg gccgcatcga catagacggc caggacattc
     4141 acgcggtgac gctgaactcg cttcgcggcc acctcgcact cgtgacgcag gagccgtttc
     4201 tgttcgacga cacgattgcc gcgaatatcg gctatggccg cgccggtgcg acacaagatg
     4261 agatcgaatc cgcagcccgt tccgctgccg cgcatgaatt catcgctgcc cttcccatgg
     4321 gttatgacac gcgcaccggc gaggggggct tgcggctttc cggcggtcag cgtcagcgta
     4381 tcgcgatcgc gcgcgc
//
LOCUS       DASYEG010000045         4045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1609280, whole genome shotgun sequence.
ACCESSION   DASYEG010000045 DASYEG010000000
VERSION     DASYEG010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609280"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VGM72_01715"
     CDS             <1..377
                     /locus_tag="VGM72_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264008.1"
                     /translation="AIPALVAASSFLAGSLTQIDFLMLIALCVTQIMLWSAIAPLIAM
                     PSMFLGGSAAAGGIALVVSIGQFGGFLGSAVIGVLKERTGDYAAAMAVIGLILALSAI
                     TVLTLGRTMTSRQVTADASRVN"
     gene            complement(344..2446)
                     /locus_tag="VGM72_01720"
     CDS             complement(344..2446)
                     /locus_tag="VGM72_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY4264009.1"
                     /translation="MTSGRANGASPCVRVERDGEIAVLVIDNPPVNASSIEVRRALLS
                     AIDSVEKDPAIEASVIIGAGTTFVAGSDIKEFGKPLEDPQLPQIIAAIQNCPKPFVAA
                     IHGAALGGGFELALGCDARVAVSNAVVGMPEVMLGMIPGAGGTQYIPRLIGVAAAIEM
                     ICSGRRVAAKEAVDSGLIDAVIESDGLWTGAVTFSRMLRGRKRPLGAEAVPTADSVAV
                     EKAAGVALRLGRNRPQTIAAIVAVKSAATLPIGEALARERSVFQALRVGTEAAALRHL
                     FFAERQAAKVTGLEGIGPRSVSNVGIIGAGMMGVGIAICFADAGIGVTFVDRDETIVQ
                     QGLARMRAIYDRMTASGRVSSDEAVRRLARVRPANDLQALSNSDLIVEAVFEEMDVKT
                     QLFHALGPILRPDAVLASNTSYLDLDRIAAATERPENVVGLHFFSPANVMRLVEVVRC
                     AGTSAQTLATALDTAKRLRKLPIVARVGEGFIGNRIFAAYRRQCEFMLEEGAYPEDID
                     AALQNFGFAMGPFAVADMSGLDIAWRMRRRLAETRDPRERYAAIADTLCERGRLGQKS
                     GAGWYRYRSGERKSQPDPEVHALIQAASVAKGFVRHAFTAEEICTRALIAMVNEAALI
                     LEEGIAARASDIDLVMVNGYGFPNYEGGPLFWARRQNRNWLLKELDTLRRMSGYGFRM
                     GNVASLHDQLTRDASAVT"
     gene            complement(2443..>4045)
                     /locus_tag="VGM72_01725"
     CDS             complement(2443..>4045)
                     /locus_tag="VGM72_01725"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEY4264010.1"
                     /translation="MGRIFVASHSGSMIGALVSRGKARGIGFAGLVSVGNEIDLSIGE
                     ICAATLDDPMVDAYLLFLETIRNSDSLRSFALAAAERGKSILAYKLGRSMAAAELAMT
                     HTGALAGEDDVADAFLKDCGIARVETLDAALEGLALLRRMPANPAAGRRPRVGVVTTT
                     AGGAAMVVDQLGIRNIAVEPPTEATLARLAEAGVDVAPARIVDLTLAGTRYLVVKSAL
                     DVLLTAPEFDLIVAVPGSSARFEPQRVVRPLIDCAASRKPLAAFVVPDAPEALAQLTA
                     AGIPNFRTPESCADSIAAAFARRTPRPLPARATAFTAGAGRLLDELEAYELLDRRGIP
                     HAPAMAVPVDSESPPTLGFPYPVAVKILSPHIAHKTEAGGVVLGVTDDRGLHRAIARI
                     RANVIKNLPETDVRRVLVQQMVRGVGEVLVGYRRDAEVGPIVILAAGGVLAEIYRDRA
                     LRLAPVGFDTAHDMIAEVKTLRALYGYRGQPAGDRDALASAIVALSQFAVLDDPRVAE
                     AEINPLLVLEEGFGVIAVDALVRLA"
BASE COUNT          788 a         1343 c         1209 g          705 t
ORIGIN      
        1 tggcgattcc cgccttggtc gccgcatcga gcttcctcgc cggaagcctc acgcaaatcg
       61 atttcctcat gctgatcgcg ctttgcgtga cgcagatcat gttgtggtcc gccatcgcgc
      121 cgctgatcgc gatgccgtcc atgttcctgg gcgggtcggc cgccgcgggc ggcatcgcac
      181 tcgttgtttc catcggtcag ttcggcggat ttctgggatc ggccgtcatc ggtgtgctga
      241 aggaacgtac cggcgactat gccgcggcca tggcggtcat cggattgatc ctcgcgctct
      301 cggccatcac cgtgctgacg ctcgggcgca cgatgacctc acgtcaagtg accgcggacg
      361 catcgcgcgt caactgatcg tgcaggctcg ccacgttgcc catgcgaaac ccatatccgg
      421 acatacggcg caatgtgtcc aattccttga gcagccaatt gcgattctgc cggcgcgccc
      481 agaacagcgg cccgccttcg taattcggga acccatagcc gttcaccatc accagatcga
      541 tatcggaggc ccgcgcggcg atgccctcct ccagaatcag cgcagcttcg ttgaccattg
      601 cgatcagcgc gcgtgtgcag atctcttcgg cggtgaacgc gtgacgcacg aaccctttgg
      661 caacggaagc cgcctggatc agggcgtgga cttccggatc gggctggctt ttccgttccc
      721 cggaacgata gcgataccaa ccggcgccgc tcttttgccc gagccggccc cgctcacaga
      781 gcgtgtcggc gattgccgca tagcgctccc tcgggtcgcg cgtctcggcg agccgccggc
      841 gcatgcgcca tgcgatatcg aggcccgaca tgtcggcaac ggcgaacgga cccatggcaa
      901 agccgaaatt ctgtagcgcg gcatcgatat cttccggata ggcgccttcc tccaacatga
      961 actcgcattg gcggcgatag gccgcgaaga tgcgattgcc gatgaagcct tcgccgaccc
     1021 gcgcgacgat tgggagtttg cgcaagcgct tcgccgtatc caaagcggtc gccagcgttt
     1081 gcgcgctcgt tccggcgcag cgcaccacct cgacgaggcg catgacattc gcaggactga
     1141 agaaatgaag accgaccaca ttctcgggcc gctcagtcgc cgcggcgata cggtcgagat
     1201 cgaggtagga cgtgttgctc gccagcaccg catcgggacg cagaatagga ccgagagcgt
     1261 ggaacaattg cgtcttcaca tccatctcct cgaagacggc ttccacaatc agatcggaat
     1321 ttgacagagc ctggagatcg tttgcggggc gcacgcgcgc gaggcgccgg accgcttcat
     1381 cggacgagac gcgccccgat gccgtcatcc gatcgtagat cgcacgcata cgcgcgaggc
     1441 cttgctgaac gatcgtctca tcgcgatcca caaatgttac gccaatcccc gcatcggcaa
     1501 agcatatggc gataccgaca cccatcattc cggcaccgat gattccgaca tttgaaacag
     1561 agcgtgggcc aatgccttcc agcccggtga ccttcgcagc ctgccgctcg gcaaaaaaca
     1621 gatggcgcag cgcggcggct tcggtcccaa cgcgaagcgc ctgaaagacc gaccgctccc
     1681 gcgccagagc ttcaccgatg ggaagcgttg ccgccgattt cacggcgaca atcgcagcga
     1741 tggtttgggg acgattgcgt cccaggcgaa gcgcaacgcc ggccgccttt tcgaccgcaa
     1801 cggaatctgc tgtgggcacc gcttccgcgc ctaacggacg tttacggcca cgtagcatgc
     1861 gggaaaaagt cactgccccg gtccaaagcc catcgctctc gattacggcg tcgatcaggc
     1921 cggaatccac agcctctttc gcggcaacgc gccgaccgct gcaaatcatc tcgatcgcag
     1981 cggcgacgcc gataagtctt gggatgtatt gcgttccccc tgcgcccgga atcatgccga
     2041 gcatcacctc gggcatgccg acgaccgcgt tcgaaacggc cacgcgcgca tcgcacccca
     2101 aggccagttc gaaaccgccg ccgagggccg ccccatggat agcggcgacg aacggcttgg
     2161 gacaattctg gatcgccgcg atgatctggg gcaattgcgg atcttcgagt ggttttccga
     2221 actctttgat gtccgatccc gcgacgaatg tggtcccggc accgatgatc accgacgcct
     2281 ctatggccgg atccttttcc acgctatcga tggcggagag gagtgcgcgg cggacctcga
     2341 tcgaactcgc gttcaccggc ggattgtcga tgacgagaac cgcaatctct ccgtcgcgct
     2401 ccacgcgcac acacggactt gcgccatttg cacggccgct cgtcacgcca gccggaccaa
     2461 ggcgtccact gcgatgacgc caaagccctc ctccagcaca agcagtggat tgatctccgc
     2521 ttcggcaacc cgtggatcgt cgaggacggc aaactgcgac agcgccacaa tcgccgacgc
     2581 gagcgcatcg cgatccccag ccggctgtcc ccgatatcca tagagcgccc gcagtgtctt
     2641 cacctctgcg atcatgtcgt gggccgtatc aaagccgaca ggcgcaagcc gcaaagcgcg
     2701 gtcgcggtaa atctcagcca gcacgccacc ggcggccaat atcacgatgg gaccaacctc
     2761 ggcgtcgcgc cggtaaccca ccagaacctc accgacacct ctgaccatct gctggaccag
     2821 cacgcggcgg acgtcggttt ccggtagatt ttttataaca ttcgctcgaa tccgcgcgat
     2881 cgcgcgatgg agaccgcgat catccgtgac gccgagcacc acgccaccgg cttcggtctt
     2941 gtgggcgata tgaggcgaca ggattttgac ggcgacggga tagggaaatc caagcgtggg
     3001 cggggattcg gaatctaccg gcacagccat cgccggggca tgcgggattc ccctgcggtc
     3061 caacaattca tacgcttcca attcgtcgag caagcgtccg gcgccggccg tgaaagccgt
     3121 ggcgcgggcc ggtagcggcc ttggtgtgcg gcgcgcgaaa gcggcagcga tcgaatcggc
     3181 gcaggattcg ggggtacgga aattcgggat acccgcggcc gtgagctgcg ccagcgcctc
     3241 gggggcgtcc ggcacgacga acgccgcaag cggctttcgc gaagccgcgc agtcgatcag
     3301 cggacgcacc acgcgttgag gttcaaagcg cgcggatgaa ccgggcaccg cgacgatcag
     3361 atcgaattcc ggcgcggtca acaacacgtc caacgcgctt ttcacaacaa ggtatcgcgt
     3421 gcccgcgagc gtgagatcga ctatgcgggc cggcgcaaca tcgacgcctg cttcagccag
     3481 tcgcgcaagc gtggcctcgg ttggcggttc aaccgcgata ttgcggatac cgagctggtc
     3541 cacgaccatc gcggccccgc ctgcggtggt ggttaccacg ccgacccgcg gccggcgtcc
     3601 cgccgccgga ttcgccggca tgcgcctgag cagcgcaagt ccttccagcg cagcatccaa
     3661 tgtttcgacg cgtgcgattc cacaatcctt gagaaaggcg tccgcaacat cgtcctcacc
     3721 ggcaagggct ccggtatggg tcatggccaa ctccgccgcc gccatggaac gtccaagctt
     3781 atacgcaaga atggacttgc cgcgttcggc cgcggccagc gcaaaacttc tcagcgaatc
     3841 gctgttgcgg atggtctcga gaaacagaag ataggcgtcc accatgggat catcgagtgt
     3901 cgcagcgcat atttcgccga tgctgagatc gatttcattg cccaccgaca caagcccggc
     3961 aaagccgatg ccgcgtgctt tgccgcgcga gaccagtgcg ccgatcatgc tgccgctgtg
     4021 agacgcaacg aaaatgcgac ccatc
//
LOCUS       DASYEG010000046         2973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3638497, whole genome shotgun sequence.
ACCESSION   DASYEG010000046 DASYEG010000000
VERSION     DASYEG010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3638497"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VGM72_01730"
     CDS             <1..206
                     /locus_tag="VGM72_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264011.1"
                     /translation="YRADINAALTLDPTFPDALVERGAMKMDAGDKAGARADFLQVLV
                     RAPDSPAADSVRKRIEALEVHDP"
     gene            complement(580..1347)
                     /locus_tag="VGM72_01735"
     CDS             complement(580..1347)
                     /locus_tag="VGM72_01735"
                     /inference="COORDINATES: protein motif:HMM:NF025221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HEY4264012.1"
                     /translation="MLGYLLTRRVVLSTVLSLAPFAALAQKSSSDPLGGLLNKIPGGL
                     GASAGSGGASLSQNQIGLGLKDALKVASQRVVGRVGKTDGYNADPDIRIPLPDALQKI
                     EGALKAVGASGMLDDLRLKMNRAAEQAAPKAFDIFVDATSKMTFDDARTILTGPQDSA
                     TQYFKRTTSGSLTSSFRPVVDTTLNGVGAVMALNTVQTKAKGIPFVGQSVGNFNLTDF
                     TVGKALDGLFHYLAVEEAAIRTNPAARTTALLKQVFG"
     gene            complement(1428..2594)
                     /gene="queG"
                     /locus_tag="VGM72_01740"
     CDS             complement(1428..2594)
                     /gene="queG"
                     /locus_tag="VGM72_01740"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEY4264013.1"
                     /translation="MRNSISETLNHADVREAIRARARAEGFDVCGFARAERPEGAAER
                     LAEYLALSHHGDMSWMQTTQARRADPHVLWPDARSIITLGVNYAPVADPLLALTRRER
                     GVVSVYAQGADYHEVLKGRLKRLASWIVQTYGGDVKIFVDTAPVMEKPLAAASGIGWQ
                     GKHTNLVARDFGSWLFLGSIFTTMNFESDPPQADSCGTCSRCLDICPTRAFPAPYTID
                     ARRCISYLTIEHKGHIAREFREAIGNRIYGCDDCLAVCPWNKFAQASRETAFHPRIEL
                     TLPLLRELVLLDDAAFREIFRGSPVKRIGRDRFIRNVLIAIGNSGHSPLAAVVEARLS
                     DESPLVRAMAVWALSRLVPREDFLALATKHAPAETDPDVREEWRHPLPGAPMAP"
     gene            complement(2575..>2973)
                     /locus_tag="VGM72_01745"
     CDS             complement(2575..>2973)
                     /locus_tag="VGM72_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY4264014.1"
                     /translation="LFDWAMNNLHEEATRRIVFEKGSQSHTGSINRRPPNMETVRLGR
                     QALKDTLPGLGTLAETRGFLAGRDVTLADLAVAAHLSALDYFGEVPWTEHLALTEWYT
                     RMKSRPSFRPLLSDRVPGQPPVSHYAELDF"
BASE COUNT          599 a          886 c          922 g          566 t
ORIGIN      
        1 gttacagggc cgatatcaat gcggccctga cgctcgaccc gacttttccg gacgcgctgg
       61 tggagcgtgg cgcaatgaag atggatgcgg gcgacaaggc cggcgcgcgc gccgatttct
      121 tgcaggtgct ggtgcgggcg ccggacagtc ccgcggccga cagtgtacgc aagcgcatcg
      181 aagcgctgga agtccacgac ccctagttcc catagcgcac gcggaccgaa gcacagccag
      241 ttagtctcta cgtccgcatg acgtgtttgc gggaacgcga tcattgagcg ggtgacggat
      301 atgagaacct tgtgccgaat ggcgcgtcag ccttgctaag gaaattccct cacaagccga
      361 actccgattg gtcggcggaa aagcgccgcg aagatctaca gcccgccaat tcgtggattg
      421 gccggtcgtc tcaattccgc gggatcgaac ctatccggcc gattgcccat gtcgtgatga
      481 agtcaaaatc ttgatcctcg acggcgcaaa ccgaatgtcg aaaccaaatc gctcatctat
      541 cgcgttgcca cccgtcacgg aaatcggcgc gtttgatcgc tagccgaaga cctgcttcaa
      601 gagggcggtg gttcgtgcgg ccgggttggt tcggatcgcg gcttcttcca ctgccagata
      661 atgaaacagc ccatcgagcg cctttccgac tgtgaagtcg gtaaggttga aatttccgac
      721 cgactggccg acgaagggaa ttcccttagc cttcgtttgc acggtgttga gcgccatgac
      781 ggcaccgacc ccgttcaacg tcgtgtcgac aaccggacgg aaggaactcg tcagggaacc
      841 cgaagtcgtt cgtttgaaat attgagtggc tgagtcctga ggaccggtga ggattgtgcg
      901 ggcatcgtcg aacgtcattt tcgatgtggc atcgacaaaa atatcgaacg ccttcggcgc
      961 cgcctgctca gccgcacgat tcattttcag tctcagatca tccaacatgc cgcttgctcc
     1021 gactgcttta agagcgcctt ctatcttctg gagcgcgtcc ggcagcggaa tacggatgtc
     1081 agggtcagca ttgtatccat ctgtcttgcc aacacgcccg acaacgcgct gcgaagcaac
     1141 tttgagtgca tctttgagac caaggccaat ctgattctgg ctcaaacttg caccgcctga
     1201 accggcactc gcgcccaaac cgcccgggat tttattgaga agcccaccca aaggatcgga
     1261 tgaagatttc tgcgcgagag ccgcaaaggg ggcgagcgaa aggacggtgg aaaggacgac
     1321 acggcgtgta agcaaatatc ctagcatgac gccctccgat gagatttcca ccatctaccg
     1381 cgcagactag cacggtttgg aacttcgccg cgaacgcacg cttggcgtta gggcgccatt
     1441 ggcgcaccgg gtaaggggtg acgccattct tcccgcacgt ccggatcggt ttcggcagga
     1501 gcgtgtttcg tggccagcgc caggaaatct tcgcgcggca ccagcctgga aagcgcccat
     1561 accgccatgg cgcgcaccag cggcgattca tcgctcagcc ttgcttccac cacggctgcc
     1621 agcggggaat gaccggagtt gccgatggcg atcagcacat tgcggatgaa gcggtcgcgc
     1681 ccgatgcgtt tgacgggcga accgcggaaa atctcgcgaa acgccgcatc gtccagcagc
     1741 acgagttcac gcagcagcgg cagggtcagt tcgatgcgcg gatgaaacgc cgtctcgcgc
     1801 gaggcttgcg cgaatttgtt ccagggacac acggccaggc aatcgtcgca gccatagatg
     1861 cggttgccga tggcttcgcg gaattcccgc gcgatatggc ccttgtgctc gatggtgaga
     1921 taggaaatgc agcgccgcgc atcgatggtg tagggcgcgg ggaaggcgcg cgtcgggcag
     1981 atgtccagac agcgcgagca ggtaccgcag gaatcggctt gtggcgggtc ggattcgaaa
     2041 ttcattgtgg tgaagatcga gcccaggaac agccacgatc caaaatcgcg cgcgaccaga
     2101 ttggtgtgct tgccttgcca gccgatgcca gatgcggcgg ccagcggctt ttccatcacc
     2161 ggggcggtgt cgacgaatat cttcacatcc ccgccatagg tttggacaat ccatgaggcg
     2221 agccgcttca ggcggccttt caacacctca tgataatccg cgccctgggc atagaccgaa
     2281 accacgccgc gttcccgccg ggtaagcgcg agcagcggat cggccacggg cgcataattg
     2341 acccccaagg tgatgatgct tctcgcatcg ggccacagca cgtgcggatc ggcccggcgc
     2401 gcctgtgtcg tctgcatcca actcatgtcg ccgtgatggc tcagcgctag atattccgcg
     2461 agtctctcgg ccgcgccttc ggggcgttcg gcacgcgcga atccacatac gtcgaaacct
     2521 tccgcgcgcg cccgcgcgcg gatcgcctcg cgaacatccg catggtttag cgtctcagaa
     2581 atcgagttcc gcataatggg ataccggcgg ctggcccggc acgcgatcgc tcagcagcgg
     2641 gcggaaagag ggtcgcgatt tcatgcgcgt ataccattcg gtcagcgcga gatgctcggt
     2701 ccatggaacc tcgccgaaat agtcgagcgc cgaaagatgg gcggccaccg cgaggtcggc
     2761 gagcgtgaca tcgcggccgg cgaggaaccc ccgtgtctcg gcgagtgtgc cgaggccggg
     2821 cagcgtatcc ttcagcgcct ggcggccgag acgcaccgtt tccatgttgg gggggcgccg
     2881 gttgatgctg ccggtgtggg actgcgatcc cttttcgaag acgatgcgcc ttgtggcttc
     2941 ttcgtggaga ttgttcatcg cccaatcgaa cag
//
LOCUS       DASYEG010000047         6406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_14795, whole genome shotgun sequence.
ACCESSION   DASYEG010000047 DASYEG010000000
VERSION     DASYEG010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6406
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14795"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VGM72_01750"
     CDS             complement(<1..952)
                     /locus_tag="VGM72_01750"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY4264015.1"
                     /translation="MPTKTIASGTVADAYLELLADRGIDYLFANAGTDFAPLIEAYAK
                     AQAHGTTVPKPVTVPHENVAIAMAQGYYLKTGRPQAVMVHVNVGTANAICGLINGWRG
                     NIPVLFTSGRTPFSEEGGLMGMRSGEIHWPQEMRDQRAMVREFVKWDYELPHGEVLEA
                     AVDRALNIAMSEPMGPVYMTLPREMLAAPIRDFRYSSPSRHATPTPAFPDTRAIDEAA
                     GMLSAAEFPLIITRSAGRVEADVAKLAGFAERFAIPVFERKHHYMCMPSDSAMNFGGN
                     PESYFDIADVIVVLEADVPWVPKHKAPREGTKIIHIGVDPI"
     gene            complement(965..1825)
                     /locus_tag="VGM72_01755"
     CDS             complement(965..1825)
                     /locus_tag="VGM72_01755"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4264016.1"
                     /translation="MSGDSMTHMEDNFITVDGLRIRYIEDGDGPPVLLLHGASLGSSA
                     DVFARNLTPLANAGFQAIAFDQPGFGLSDVPTDHSTAYRRDIVPKFLDALRLKQAALV
                     GHSQAGNPAVQLALREPERYTHIVVLGTGSLLPPLDGAGEDRGAAAQQRLERRMAESE
                     PTIEDTRKLLEANLFHHELITPEALALRHARSIGRNFQAFVARNAAAEAAPPKEPAVP
                     LWQRLTSLKMPLLLIFGRDDRANAAERAELLMEKYPDLDVNIVDDCKHLVPWDAADEF
                     IRLAVPFLKS"
     gene            complement(1875..2687)
                     /locus_tag="VGM72_01760"
     CDS             complement(1875..2687)
                     /locus_tag="VGM72_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4264017.1"
                     /translation="MFAQINHMAIISPAYANLEKFYQAVFGLKPSARTRPGSAATTGD
                     GYVGLNLIPRRDGYVGGIDHFGMVVDDVAPVLERMQKKYPRANLVKRPSARPFAAYSG
                     HDPDGNVFDLAQKKQDTRKEVYADIAAEGWAQDRYFNKFAIRTPNAEQCAEFYADVFE
                     LQPVNRKGRSPGYHLTDGRVTLSILPWSIPVFEGMSIKRPGPDHVGFHVEDLEGFKKH
                     VAEAAGANPILAPVPLGGSPESDVRRRFFEKHATGKWQMSDPDGNWIDITDE"
     gene            complement(2700..3509)
                     /locus_tag="VGM72_01765"
     CDS             complement(2700..3509)
                     /locus_tag="VGM72_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4264018.1"
                     /translation="MFAQINHTAIISHQYNALSKFYEAVFGLKYSAFRLTDAPIVSDG
                     YVGMQLIPRRDGYVGGIDHFGMVVDDVETVLERMQKKHKRANIVKRPSTRPFAAYSGH
                     DPDGNVFDLAQKKNDTRVDLYKKQAEEGWAQDRYINKFAIRTPNAEACAEFYADVFEL
                     QPVNRESEAPGYHLTDGRITLSILPWSIPIFANMSIKRPGPDHLGFHVEDLEGFKKHV
                     AEVAGANPYLAPVPLGGSPESKVRQEFFKTSATGKWQMTDIDGNWLDITDE"
     gene            complement(3540..4949)
                     /locus_tag="VGM72_01770"
     CDS             complement(3540..4949)
                     /locus_tag="VGM72_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4264019.1"
                     /translation="MTKPVDVADIIDAQPVSFYQCLIAFMAWLTLFLDGVDNQSIAYV
                     APALTHDWGLGRGALRTVFSSGVLGVAIGALIIGPLADRYGRKRVTVLTIIYVALFSL
                     IVTQVPEISALLSPAFAGVSDLNVLMVLRFLTGLGLGAAVPLSVVIVNEFAPKRRRAA
                     MVTLMGCGYAMGSASGGLLASHLVPAFGWQAQFYVAAAMTLVMAIVLAAWLPESIRLL
                     TVQGKSAGVIAILRKINPALSFEEGTHFVLRQELREKSAHRLRPLRLFSEGRSSVTVL
                     IWLCLFMNTLALNYLNNWLPTLTTETGLPEAQALRAAASLQFGGILGVITLGFLADRY
                     GFFKVLAGSFAGGGVCIALIGWAGGAFYPLSATIFASGFFNIGTQITLAALAATLYPT
                     DIRSTGVSWAHGVARIGSIISIMFAGIMLALHWPLQTMYYLVSIPMFFGCVWIFILAN
                     VRARTSYVAAEPQRRRAMA"
     gene            complement(5063..5707)
                     /locus_tag="VGM72_01775"
     CDS             complement(5063..5707)
                     /locus_tag="VGM72_01775"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEY4264020.1"
                     /translation="MTPGRAKSFIMQHRQNTRQRNSVLKLKVAANCPDWDTRIAIIGA
                     CTQEVIADHEHGHGKAHWQILEELGTHIGMSLNDIRAAKPIDTTQIAWAAWEGLMANR
                     HWLEGIVANTCAERANVPGYGDGVMRDKGWFGLERERWGALFTLDNEQLDFFELHEQA
                     DIEHSDMGWQTIAKFSRDLHMEDAVVEACRKNLLVWELYLNGIAAAGDAVDRKI"
     gene            complement(5888..5963)
                     /locus_tag="VGM72_01780"
     tRNA            complement(5888..5963)
                     /locus_tag="VGM72_01780"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5928..5930),aa:Thr,seq:tgt)
     gene            complement(6151..>6406)
                     /locus_tag="VGM72_01785"
     CDS             complement(6151..>6406)
                     /locus_tag="VGM72_01785"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4264021.1"
                     /translation="MYGSKNSAGNYSFLYSAKGFAALLGGGLGAQLFESTGTWDYAFF
                     ASAALALLSAFMALYLLWMPLPQKSPSSVAEGKPAPLPAE"
BASE COUNT         1278 a         1971 c         1956 g         1201 t
ORIGIN      
        1 agatcgggtc gacaccgata tggatgatct tggtgccctc ccggggcgcc ttgtgcttgg
       61 ggacccaggg cacatcggct tccaggacca cgatgacatc ggcgatatcg aaatagcttt
      121 ccggattgcc gccgaaattc atcgcgctgt cggacggcat gcacatgtaa tgatgcttgc
      181 gctcgaacac cggaatggcg aaacgctccg cgaagcctgc gagcttggcc acatcggctt
      241 ccacgcgccc cgcgctgcgg gtaataatga gcggaaattc ggccgccgac agcatcccgg
      301 cggcctcatc aatggcgcgg gtgtcgggaa aagccggtgt cggcgtggca tggcgcgacg
      361 gcgaggaata gcggaaatcc ctgatggggg ccgccagcat ctcgcgcggc agcgtcatat
      421 agacaggacc catcggctcg ctcatcgcga tgttcagcgc ccggtcgacg gcggcttcca
      481 gcacctcccc atggggaagc tcataatccc atttgacgaa ttcgcgcacc atcgcacgct
      541 gatcgcgcat ctcctgcggc caatggattt cgccactgcg catgcccatc agcccgccct
      601 cttccgagaa cggcgtgcgg cccgaggtga acagcactgg aatattgccg cgccagccat
      661 tgatcagacc gcagatcgcg ttggcggtac cgacattgac atgcaccatc accgcctgcg
      721 gccggcccgt cttgaggtaa tacccctgcg ccatcgcgat ggcgacattc tcatggggca
      781 cggtgaccgg cttgggcacc gtggtgccgt gcgcctgggc cttcgcatag gcctcgatca
      841 gcggcgcgaa atccgtgccc gcattggcga agaggtaatc gatgccgcgg tcggcgagca
      901 attccagata ggcatcggcg acggtgccgg acgcgatggt tttggtcggc acgaagaact
      961 ccgattacga tttcaggaag ggcacggcaa gccggatgaa ttcatccgcc gcgtcccacg
     1021 gcaccagatg cttgcaatca tccacgatat tcacatcgag atcgggatat ttctccatca
     1081 gcagctcggc gcgttccgcg gcattggcgc ggtcgtcgcg cccgaagatg agcagcagcg
     1141 gcattttgag cgacgtcaga cgttgccaca gcggcaccgc cggttccttc gggggtgctg
     1201 cttcagccgc cgcattgcgc gccacgaagg cctggaaatt ccggccgatg ctgcgcgcat
     1261 ggcgaagcgc cagcgcctcg ggcgtgatca gttcatgatg gaagagattg gcctccagca
     1321 gcttgcgcgt atcttcgatt gtgggttcgg attccgccat gcgccgttcc agccgctgct
     1381 gcgcggcggc gccgcgatcc tcgccggccc cgtcgagcgg cggcaacagg ctgcccgtgc
     1441 cgagcacgac gatgtgggtg tagcgctcgg gttccctcag cgcgagctgc accgccggat
     1501 tgccggcctg cgaatggccc accagtgccg cctgtttcag ccgcagcgcg tccaggaatt
     1561 tgggcacgat atcgcggcga taggcggtgg agtggtcggt tggcacatcg gaaagaccga
     1621 accccggctg atcgaaggca atggcctgga acccggcatt cgcgagcggt gtgagattgc
     1681 gcgcgaacac atcggccgac gagccgagcg acgccccatg cagcagcagc accggcggac
     1741 cgtcgccgtc ctcgatatag cggatcctga gcccgtcaac cgtaatgaaa ttgtcttcca
     1801 tgtgcgtcat ggaatcgccg ctcacgctga cctccttgtt tgagtcgggt gcttcatatc
     1861 ataggctcac gaaattattc gtccgtgatg tcgatccaat tgccgtccgg atcgctcatc
     1921 tgccatttgc cggtggcgtg cttttcgaag aatcgccggc gcacgtccga ttccggactg
     1981 ccgccgagcg gcaccggcgc gagaatgggg ttggcgcccg cggcctcggc gacatgtttc
     2041 ttgaagcctt ccagatcctc gacatggaag ccgacatggt ccgggccggg acgcttgatc
     2101 gacatgcctt cgaacacggg aatggaccag ggcaggatcg acagcgtcac ccgtccatcc
     2161 gtcagatgat aaccgggcga ccgccccttg cgattcaccg gctgcaactc gaacacatcg
     2221 gcgtagaact ccgcgcattg ttcggcgttc ggcgtgcgga tggcgaattt gttgaaatag
     2281 cgatcctgcg cccagccttc ggcggcgatg tccgcataga cctccttgcg cgtatcctgt
     2341 ttcttctgcg ccagatcgaa gacattgccg tccggatcat gcccgctata ggccgcgaag
     2401 gggcgcgcgg agggacgttt caccaggttc gcgcggggat attttttctg catgcgttcg
     2461 agcacgggcg cgacatcgtc caccaccatg ccgaaatgat cgataccccc gacataaccg
     2521 tcacggcgtg gaatcagatt gagtccgaca tatccgtcgc cggtcgtggc cgccgaaccg
     2581 ggccgcgtcc ttgccgaagg cttcaacccg aacacggcct gatagaattt ttccagattc
     2641 gcgtaggccg ggctgatgat cgccatgtgg ttgatttgcg caaacatcgg cacaatctct
     2701 cattcgtccg taatgtcgag ccaattgccg tcgatatccg tcatctgcca tttgccggtg
     2761 gcgctggttt tgaagaactc ttgacgcacc ttggattcgg gactgccgcc gagcggcacc
     2821 ggcgcgagat agggattggc gcccgcgacc tccgccacat gcttcttgaa cccttcgagg
     2881 tcctcgacat gaaagcccag atgatcggga ccgggacgct tgatggacat attggcgaag
     2941 atcgggatcg accacggcag gatggaaagc gtgatgcgtc cgtccgtcag atgatagccc
     3001 ggcgcttcgc tttcgcggtt caccggctgc agctcgaaga catcggcgta gaattccgcg
     3061 caggcttcgg cgttcggcgt gcggatggcg aatttgttga tatagcggtc ctgcgcccaa
     3121 ccctcctcgg cctgtttctt gtagaggtcg acgcgggtgt cgtttttctt ctgtgccaga
     3181 tcgaagacgt tgccgtccgg gtcatggccg ctataggcgg cgaaggggcg tgtggaagga
     3241 cgcttcacga tgttggcgcg cttgtgcttc ttctgcatgc gttccagaac ggtttccaca
     3301 tcgtccacca ccatgccgaa atgatcgatg ccaccgacat agccgtcgcg gcgcgggatc
     3361 aattgcatgc cgacatatcc gtccgagacg atcggcgcgt cggtaagacg gaaggcggaa
     3421 tatttcagac cgaacacggc ctcgtaaaac ttgctcagcg cattatattg gtgactgatg
     3481 atggcggtat gattgatctg ggcgaacatt cttttctccg gtcttttttg tcttttctct
     3541 cacgccatgg cgcggcggcg ttgcggctcg gcagcgacat acgaagtcct ggcgcgcaca
     3601 ttggcgagga tgaatatcca cacgcagcca aagaacatgg ggatcgacac cagatagtac
     3661 atcgtttgca gcggccaatg cagcgccagc atgatgccgg cgaacatgat gctgatgatc
     3721 gacccgatgc gggcgacgcc atgcgcccaa ctgacgcccg tcgatcggat gtcggtgggg
     3781 tacagcgtgg cggcgagcgc ggccagcgtg atctgcgtgc cgatgttgaa gaagcccgat
     3841 gcgaagatgg tcgcggagag cggatagaac gccccgcctg cccagccaat cagcgcgatg
     3901 cacacgccac cgcctgcaaa ggatccagcg agcaccttga agaaaccgta tcggtcggcg
     3961 aggaacccca gcgtgatcac gcccagaatg ccgccgaatt gcagcgaggc cgccgcgcgc
     4021 agtgcttgcg cctcgggcag gccggtttcg gtcgtcaaag tcggcagcca attgttcaga
     4081 tagttcagcg cgagcgtgtt catgaacaga cagagccaga tcagcaccgt cacgctgctg
     4141 cgcccttcgc tgaaaagacg cagcggacga agccgatggg cgcttttctc ccgcaactcc
     4201 tggcgcagca cgaaatgcgt gccctcctcg aacgacaaag caggattgat cttgcgcaaa
     4261 atggcaatga cgccagcgct tttgccctgc accgtcagga gccggatcga ttccggaagc
     4321 cacgccgcga ggacaatcgc catcaccagc gtcatcgccg cggcaacata gaattgcgcc
     4381 tgccagccga atgccggaac cagatgggac gcgagcagcc cgcccgaggc cgagcccatc
     4441 gcgtagccgc accccatcaa tgtgaccatg gcggcgcggc ggcgcttggg cgcgaattcg
     4501 ttgacgatca cgacagacag cgggacagcg gccccgagac cgagtccggt caggaaacgc
     4561 aacaccatca agacattcag gtcggatacc cctgcgaacg ccggcgacag cagtgccgaa
     4621 atctccggca cctgggtgac gatgagggag aacagcgcca catagatgat ggtcagaacc
     4681 gtgacgcgct tgcggccata gcggtcggcc agcggcccga tgatgagcgc gccgatggcc
     4741 acgcccagca cgcccgatga gaaaaccgtg cgcagcgcgc cgcgtcccaa accccaatca
     4801 tgggtaaggg cgggcgcgac ataggcgatg gactgattgt cgacgccgtc gagaaacagg
     4861 gttagccatg ccatgaaggc gatgaggcat tgatagaaac tcaccggctg agcatcgatg
     4921 atgtccgcga cgtcgaccgg cttggtcatc tctaacgcct cccactggcc tttggcctaa
     4981 tcgggtaatc ctacacgcaa aaaaccgcgc tgtcccagag gcatagacca aacgcacccc
     5041 gcaaaagcac cgcaaaaaga gatcagattt tgcggtcaac cgcgtcgccg gcggcggcga
     5101 tgccgttcag atatagctcc cataccagga ggttcttgcg gcaggcctca acgaccgcgt
     5161 cttccatatg caggtcgcgt gagaatttgg cgattgtctg ccagcccata tcggaatgct
     5221 cgatgtcggc ctgctcgtgc agttcgaaga aatccagttg ctcattgtcg agcgtgaaca
     5281 gtgcgcccca gcgttcgcgc tcgagcccga accaaccctt gtcacgcatg acgccatcgc
     5341 cataaccggg cacattggcg cgctccgcgc aggtgttggc gacgatgcct tccaaccaat
     5401 ggcggttggc catcaatcct tcccaagcgg cccaggcgat ttgcgtggtg tcgatcggtt
     5461 tcgccgcgcg gatgtcattc aggctcatgc cgatatgggt gccgagctct tccaggatct
     5521 gccaatgcgc cttgccgtgg ccatgctcgt gatcggcgat gacctcttgc gtgcaagcgc
     5581 cgatgatcgc gatgcgcgtg tcccaatccg ggcaattggc ggcgactttc agtttcagga
     5641 cagagttgcg ctgacgcgtg ttctgccggt gctgcatgat gaaagatttc gcgcggcctg
     5701 gcgtcatcgg ctccttgaaa aaacgagtcc ggcgacaaaa atcatcgacc actgcgtcca
     5761 ggcggcgaag gatcgggtct tgctgagagg ccatcgaatg ttcctgaagg gtcggcacac
     5821 gcgcgttctt gcatgcctgt ttgcgaagcg aaattcgagg atcctcaccc ggacactccg
     5881 aagaggctgg tgccggctgc aggatttgaa cccgcgaccc cgtgattaca aatcacgtgc
     5941 tctaccaact gagctaagcc ggcccatggc tttgcgacat acgacgaatg cgggcgagtg
     6001 ggcaatcgcg cgtctttcaa tttccgattg ccggaatgaa gagccatgcc tccccgatgc
     6061 acaaccaagc gagacgccat cgaaccggtg gcagaccgaa gcagcagcgg cgacgccgat
     6121 gcgctcgccc acgcggcgtt gcggatcgat ctactccgcc ggaagcggtg ccggtttccc
     6181 ctcggcgacg gagctcgggc tcttttgtgg caaaggcatc cagagcagat acagcgccat
     6241 gaaggccgag agcagagcga gggcggcgct ggcaaagaac gcataatccc aggtgcccgt
     6301 gctttcgaac agctgcgcgc cgagcccccc gcccaaaagg gccgcgaaac ccttagcact
     6361 atataagaag ctgtagttgc ctgccgagtt cttgctgcca tacatg
//
LOCUS       DASYEG010000048        12605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_449106, whole genome shotgun sequence.
ACCESSION   DASYEG010000048 DASYEG010000000
VERSION     DASYEG010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 12605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12605
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449106"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VGM72_01790"
     CDS             complement(<1..386)
                     /locus_tag="VGM72_01790"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4264022.1"
                     /translation="MENRAHLPVPLTYPKLLTLMEEAGVGRVILVPPSLDGDRNDLCL
                     AAASARPDKFAVMGRIFVDRPSAREKLAEMKHQNGMLGLRLTFHRDNDRPLLTNGAAD
                     WLWPEAERLGMPVMIHAPERLPVIGA"
     gene            complement(460..1374)
                     /locus_tag="VGM72_01795"
     CDS             complement(460..1374)
                     /locus_tag="VGM72_01795"
                     /inference="COORDINATES: protein motif:HMM:NF018420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HEY4264023.1"
                     /translation="MRGNRTGFLGALALGATLALVDSTAASAAAAAPSTPSSQAAEVD
                     VALILAIDASGSIDHDEAELQRKGISEAFLSKEVLQAIQSGSLGRIGVAAIYFSSRQY
                     GVMSVPVNWMMIRDQKSAEDFVRTLVAAPRQSARGTSISDALELSQRMLEKGPYHSAK
                     QVIDVSGDGENNAGRPMLEVRDELLAKSITINGLPIIDETTSQDLDKYYEGCVIGGPG
                     SFVIPAKGFGDFARAMRRKLILEISGLSPRETPGANPLLKKVAATAPARPLPRGGYVA
                     KNPPPPRGCDFPMFGGFGGFGGFSFPER"
     gene            1518..2627
                     /locus_tag="VGM72_01800"
     CDS             1518..2627
                     /locus_tag="VGM72_01800"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEY4264024.1"
                     /translation="MEDNLLAVARAGKKRHPYHRRRRGGQGARALSNGVPVLGAIDVG
                     TNACRLLIAIPQMHLTGEYGGMVPRVIDSYTANVRLGESLERTGTITEAALDRTVAAL
                     KVCAARLRRRNVTHVKAIATEACRRASNAQDLVKRAEAEAGIRLTIVTPEEEARLAAA
                     GCLQLIGHDFEGALIFDIGGGSTELILVRRDGVRRPAQYEIVAWSSAPVGVVKLAERH
                     GSHELAAASYKPMRAELDAIFSAMRAELGPDMFDAGRYHLLGTSGTLTTLAGIKLGLK
                     RYERSRIDGQWLKRDDIVQISRDIVTRDFSGRAAIACIGTDRADLILPGCAILDSIMD
                     NWPCATLRVADRGLREGMLIALLRESEAATAERTQ"
     gene            2627..3313
                     /locus_tag="VGM72_01805"
     CDS             2627..3313
                     /locus_tag="VGM72_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEY4264025.1"
                     /translation="MSPKSVPPKSPGARGLRVRVRSKGLKASSTRWLARQLNDPYVAE
                     AKAKGYRSRAAFKLIELDDKFHFFKPGARVLDLGAAPGGWSQVARARVGAKGSVVAAD
                     ILEMEPLSGVHLLMLDMLEEEAPAAIRQALAGPADIVLSDMAAATTGHRATDHLRTTG
                     LFEAALELASEVLRPGGAFVGKVFQGGAAPELLVRLKKSFREVRHVKPPASRAESVEL
                     YLVALGFKAL"
     gene            3388..3786
                     /locus_tag="VGM72_01810"
     CDS             3388..3786
                     /locus_tag="VGM72_01810"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEY4264026.1"
                     /translation="MATIYTDANRGADVPREETFSLIASDKVEGTAVYGPDREKIGSV
                     DNLMIDKHSGRVAYAVLSFGGFLGMGTDHYPLPWSVLRYDEDLGGYVTNITKDQLTGA
                     PKYRDDENWDWNDQEYIGMVDTHYRPIAFI"
     gene            3930..5399
                     /gene="guaB"
                     /locus_tag="VGM72_01815"
     CDS             3930..5399
                     /gene="guaB"
                     /locus_tag="VGM72_01815"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY4264027.1"
                     /translation="MTIREALTFDDVLLVPAASTVLPGQVDTRTRLTKTIELGIPLIS
                     AAMDTVTEAGLAIAMAQAGGMGVIHRNLTPEEQAGQVRRVKKFESGMVVNPLTIGPEA
                     TLADALSLMERNHISGIPVVESTGRPGTLVGILTNRDVRFAQDRRQSVRELMTKERLV
                     TVRENVPMEEAKQLLHQHRIEKLLVVDESYRCVGLITVKDIEKAQKYPNACKDERGRL
                     RVAAATNVGEDGVARAITLLEAECDVIVVDTAHGHSKGVLETVARIKRESNYAQVVAG
                     NIATADGAKALIDAGADAVKVGIGPGSICTTRIVAGVGVPQLTAVMDAAEAARKQGIP
                     VVADGGIKYSGDLAKAIAAGADVAMIGQLLAGTDESPGEVFLFQGRSYKGYRGMGSLG
                     AMARGSADRYFQAEVKDTLKLVPEGVEGQVPYKGPAGAVVHQIVGGLRAAMGYTGCAT
                     IDDFHEKAEFVRITSAGINESHVHGVVVTRESPNYPRPQ"
     gene            5415..6485
                     /locus_tag="VGM72_01820"
     CDS             5415..6485
                     /locus_tag="VGM72_01820"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEY4264028.1"
                     /translation="MTTVRKGQAPGPMSRDAFHAHFRRSFFDPAFDCEIDAIARLEEI
                     AWNAYCKGRKAPRAQKAGAGFADPDYDLSIEWHETRDALIVAEARQKDSTAPSRVLLI
                     IGSARNDGTCPGEISKSFRLANAAEEIFGAAGLNVDRLDLSLLSSDYGRKIHPCKSCV
                     ATSMALCHWPCSCYPNHALGQSDDWMAEIYQRWVAAHGVMIVTPVYWGQAPSGLKLMM
                     DRLVCADGGNPDPSSTHGKKPEEAKALELAGWPYPKHLAGRVYGVAVQGDAAGVQEVR
                     SALCDWLDWNGLIGAGALSQLDRYIGYFEPYATSHEALDKDAAVVEEVKNVARAIARA
                     IPEMRKGKLKPPDSGLTEPRKK"
     gene            6539..7810
                     /locus_tag="VGM72_01825"
     CDS             6539..7810
                     /locus_tag="VGM72_01825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEY4264029.1"
                     /translation="MTPAARIQAAIEVLDALNATTMPADRLLRDWFRARHFMGSKDRA
                     AVAERVYAVLRHRASAGWRMGAEDSRSLAIASLLGEGLTGDEIAKLFAAGAYGPSPLT
                     ENESRAISEPPAGPAPLHVLGEFPQWLEPELTRAFGDRLLEHMRAMLTRAPVDLRVNT
                     LRASRADMLVGLRSLDVVADLAPFAPHGIRIPSGEGLGALQQTQFFQTGAFEFQDESS
                     QIAAVLCDAKPGSSVLDLAAGSGGKSLALAAAMQNTGEILAFDTDATRLKQLRPRARR
                     AGATIIAATGNLGGPLWGEEKFDCVLVDAPCSGSGAWRRNPGAKWWLTRERLDSLMAL
                     QNRLIDDGARHVKPGGRLVYATCSVLPCENADVIAHFLARNPGFRVLAAEEIWRSATA
                     SAPPPGMKDYFTANPLMTGMDGFFACIMSTD"
     gene            7843..8334
                     /locus_tag="VGM72_01830"
     CDS             7843..8334
                     /locus_tag="VGM72_01830"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4264030.1"
                     /translation="MNRSLLGIILAAGLTSSAAWAAASAPQKPADAESDSQAAQLKPQ
                     DMHFLTDADQAAKAGDSDGAVQLYHSAIIYAPGDPVPYERLAEFYVHSNRPEQAQRYF
                     TLALDAQPAYAPALQGLALLDIAAGNRAAAQAQHDILLHSCGAKCPETAQVEKALNSR
                     SAP"
     gene            8367..9911
                     /gene="guaA"
                     /locus_tag="VGM72_01835"
     CDS             8367..9911
                     /gene="guaA"
                     /locus_tag="VGM72_01835"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEY4264031.1"
                     /translation="MPPVLVIDFGSQVTQLIVRRVRESGVYCEVVPFNKAEAALAEGI
                     PGAIILSGGPASVGDAGSPRAPESVFTLGVPVLGICYGQQAMVTQLGGRVEPHHKREF
                     GRAEIEVSGQSPLLEGVWPLGSHDTVWMSHGDRVIQLPPGFHVLAQSDNAPFAVIADE
                     ARKFYGVMFHPEVVHTPKGGALLRNFTQIVAGIAPDWNMKAFRAEAVRRIRAQVSGAR
                     VICGLSGGVDSSVAALLIHEAVGDALTCIFVDTGLMRSGEAQDVVSLFRGHYNIPLVH
                     ADAGELFLSRLEGIDDPEKKRKIIGATFIDVFDAEAKKVGHPEFLAQGTLYPDVIESV
                     SAIGGPSVTIKSHHNVGGLPERMHLKLVEPLRDLFKDEVRALGRELGLPQSFVGRHPF
                     PGPGLAIRIPGAITPEKLDILRRADVIYLDEIRKAGLYDAIWQAFAVLLPVKTVGVMG
                     DDRTYEYVCGLRAVTSSDGMTAESYPFEHAFLARVATRIINEVRGINRVVYDVTSKPP
                     GTIEWE"
     gene            10204..11484
                     /locus_tag="VGM72_01840"
     CDS             10204..11484
                     /locus_tag="VGM72_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY4264032.1"
                     /translation="MLTDAALKHLKPKDKLYKVTDRDGMYVLVRPSGVLTFQLDYRMN
                     GRRETVTFGKYGPADLSLARARELCIDAKRAIAEGRSPAIEKQRDKRRLLEAKSFGEF
                     GEKWLTAAPMADSTRAMRRSIFERELLPVWRNRLLKEITPDDLRAHCGAIVERGAPAT
                     AIHVRDILKQIYGFAILHGEKVANPADDVGPASIATFVPKDRSLSPTEIRIMLKLLEK
                     VATLPTIRLGMRLYLQTMVRKSELQDAIWDEVDFENAVWTIPKERMKRSKAHNVYLSR
                     QTLDIMIALKTCAGNSRYLLPSRYDADAPMSRATFNRVTYAVVEQAKKEGLPLEPFTV
                     HDLRRTGSTLLNELGFNSDWIEKCLAHEDGRSSRGVYNKAEYEVQRRHMMQEWSDIID
                     AWVEGKKRVPVLIPPSMPLLEPDPVLEPGAIAAA"
     gene            complement(11414..11698)
                     /locus_tag="VGM72_01845"
     CDS             complement(11414..11698)
                     /locus_tag="VGM72_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HEY4264033.1"
                     /translation="MSTTIKRTIRRQQLREIVPLADSTIYEMEQRGEFPQRFALSPRC
                     VVWDLAEVEAWLVSRRSAPIVRAQAPDVRQRRSRPVREPGRAQAAASKAG"
     gene            11895..12170
                     /locus_tag="VGM72_01850"
     CDS             11895..12170
                     /locus_tag="VGM72_01850"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY4264034.1"
                     /translation="MAKLAICSRGGETLKLYYSRNLNPRVAVAVARYLELPVEFVRAS
                     PREAKNEDAFRRINPNALVPVLVEENRSLWETDAIACRLAMLANSDF"
     gene            12201..12545
                     /locus_tag="VGM72_01855"
     CDS             12201..12545
                     /locus_tag="VGM72_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEY4264035.1"
                     /translation="MWVSWSTHHFTRAASVFYWEYVIKPMIGLGSASESAIEEATGDF
                     HRFAGVLDDALSGRTWLVGDNPSYADFRVATALPFAEASKLPLREYKNIGKWHDRLWR
                     FPAWSDPFAGLA"
BASE COUNT         2392 a         3817 c         4013 g         2383 t
ORIGIN      
        1 atcgcgccga tcaccggcag gcgctcgggc gcatggatca tcaccggcat tcccaagcgt
       61 tccgcttccg gccacagcca atccgccgcg ccattggtga gcagcgggcg gtcattgtcg
      121 cggtgaaagg tcaacctcag ccccagcatg ccgttctgat gcttcatctc ggcgagtttt
      181 tcgcgggccg atggcctgtc gacgaaaata cgtcccatga cggcgaattt atcgggacga
      241 gcggacgccg ccgcgagaca cagatcgttg cggtcgccgt cgagtgaagg cggcacgagg
      301 atcacgcgcc cgacgccggc ttcctccatc agggtgagaa gcttcggata agtcagcggc
      361 accggcagat gggcgcggtt ttccattccc tcgccccagg ggcgctcggg cgtgttggcg
      421 gcccagatgt ggacctgcgt atcgacgacg agaatgggcc taccgctccg ggaaactgaa
      481 tccgccgaag ccgccaaacc cgccgaacat cgggaagtcg cagccgcggg gcggcggcgg
      541 attcttcgcg acatagccgc cacgcggcag cggacgcgcg ggcgcggttg ccgccacttt
      601 cttcagcagc ggatttgcgc ccggcgtttc gcgtgggctc agtccggaaa tctccaggat
      661 cagcttgcgc cgcatcgcgc gcgcgaagtc gccgaagccc ttggcgggaa tcacgaacga
      721 gccggggccg ccgatcacgc agccctcgta atatttgtcg agatcctgcg aggtggtttc
      781 atcgatgatg ggaagaccat tgatggtgat gctcttggcg agcagttcat cgcgcacttc
      841 aagcatcgga cggccggcat tattttcgcc atcgcccgag acgtcgatca cctgtttggc
      901 ggaatgatag ggaccttttt ccagcatgcg ctgcgacagt tccagtgcgt cggagatcga
      961 ggtgccacgc gccgactgac ggggcgccgc gaccagcgtg cgcacgaaat cttccgcgct
     1021 tttctgatcg cggatcatca tccagttcac cggcacgctc atcacgccat attgccggct
     1081 ggaaaaatag atggcggcga caccgatgcg gccgagcgag cccgactgga tcgcctgaag
     1141 aacctccttg ctgaggaacg cctcggaaat ccccttgcgc tgcagctccg cctcgtcgtg
     1201 atcgatgctg cccgaagcat cgatggcgag tatgagggcg acatcgactt cggccgcctg
     1261 gctggacgga gtggaagggg ccgctgcggc cgccgatgcc gccgtggagt ccaccagcgc
     1321 cagcgtggcg cccagcgcca atgcgcccag aaagcctgtc ctgtttccac gcatgacccg
     1381 ctcgccacac agccgcaagc gaaaatctat acagaagcgg ctggaacggc aatcttcatg
     1441 gctattatgg acgatctgtc ataggcctac atgaatgcgg gttgcacgga gctgttctcc
     1501 gggccaagct tcgaaaaatg gaggacaatt tgctggcggt cgcgcgcgcc ggaaagaaac
     1561 gtcatcccta tcaccgccgc cggcggggag ggcagggcgc acgcgcgctc agtaatggcg
     1621 ttccggttct cggcgcgatc gatgtcggga cgaatgcgtg ccgtcttctc attgccattc
     1681 cgcagatgca tctgaccggc gagtatggcg gcatggttcc gcgcgtaatc gattcctaca
     1741 ccgccaatgt ccgtttgggt gaaagtctcg agcgaaccgg aaccatcacc gaggccgcac
     1801 tcgatcgcac ggtcgcggca ctcaaggtct gcgccgcgcg tctgagacgc agaaacgtca
     1861 cccatgtcaa agccatcgcc accgaagcct gccgccgcgc gagcaatgcg caggatctgg
     1921 tcaaacgcgc cgaagccgaa gctggcatcc ggcttaccat cgtgactccg gaagaggagg
     1981 cgcgtcttgc cgcggccggc tgcctgcaac tgatcggcca cgatttcgaa ggcgctctca
     2041 tcttcgatat cggcggcggc tcgaccgagc tgatattggt gcggcgcgac ggcgtgcgcc
     2101 gcccggcgca atatgagatc gtcgcctgga gctccgcgcc cgtaggggtc gtaaagctcg
     2161 ccgagcggca tggcagccat gagcttgccg cggccagcta caaacccatg cgcgcagaac
     2221 tcgacgcgat cttctccgcc atgagagctg agcttggacc ggacatgttc gacgcggggc
     2281 gctatcatct gcttggcacg tcggggacgc tgaccacgct tgccgggatc aagctgggat
     2341 tgaaacgcta cgagcgcagc cgtatcgacg gacaatggtt gaagcgagac gacatcgtgc
     2401 agatttccag ggatatcgtg acgcgcgatt tttccggccg cgctgccatt gcctgcatcg
     2461 gcacggaccg ggcggatttg atccttcccg gctgcgccat tctggactcc atcatggaca
     2521 attggccttg cgccaccttg cgggtggccg acaggggcct gcgcgaggga atgctgatcg
     2581 cgctcctgcg tgaatcggaa gccgcgaccg cggagcgcac gcaatagtgt cgccaaaatc
     2641 ggtgccgcca aaatcaccgg gcgcgcgggg tttgcgcgta cgtgtccgat ccaaagggct
     2701 gaaggcatcg tccacgcgct ggctggcgcg ccagctcaac gatccctatg tcgccgaggc
     2761 caaggccaag ggctaccggt cacgcgcggc gttcaaactg atcgagctcg acgacaaatt
     2821 ccatttcttc aaacccggtg cgcgggtcct cgacctgggc gccgcccccg gcggttggag
     2881 ccaagtcgcg cgcgcgcgcg tgggcgccaa gggaagcgtg gtggctgcgg acattctcga
     2941 gatggaaccg ctttccggcg tgcacctgct gatgctcgac atgttggagg aagaggcgcc
     3001 ggctgcgatc cggcaggcgc tcgcagggcc ggccgacatc gtactctccg acatggcggc
     3061 ggccaccacg ggccatcgcg ccaccgatca tctgcgcaca acgggacttt tcgaagcggc
     3121 tctggaattg gcgtcagagg ttctgaggcc aggcggtgct tttgtcggca aagtgttcca
     3181 aggcggcgcc gcgcctgaat tgcttgtccg gctgaaaaaa tcctttcgcg aagtgcggca
     3241 tgtgaagcca ccggcgagcc gcgcggaatc ggtggagctt tatctggtgg cgttgggatt
     3301 caaggcgctc tgacttctct tgccgtaagc ggaactaggg atggggccgc acgttccttc
     3361 ggcgtgcggg ggctttggag gacgaacatg gcgacaattt atacggatgc aaaccgcggc
     3421 gcagacgtgc ctcgggaaga aacgttttcc ctcattgcaa gcgacaaagt ggaaggaacg
     3481 gcggtctacg gaccggaccg cgagaagatt ggcagtgtcg acaatttgat gatcgacaag
     3541 cacagcgggc gcgtcgccta cgcggtgctc agtttcggcg gttttcttgg aatgggaacg
     3601 gaccactacc cgcttccttg gtcggttctg cggtatgacg aagaccttgg cggatatgtc
     3661 accaacatca ccaaggatca gctgacgggc gccccgaaat atcgcgatga cgagaattgg
     3721 gattggaacg accaggaata tatcggcatg gtcgatacgc attacaggcc catagccttt
     3781 atctagtccg gtctttgacg agaattcccg gcgaggtctc tcttgaaggg ggcctcgctt
     3841 gtttttgtgg ctttccccgc agaaccgtcc gcgctcttgc taagaaacgg gcgcgatgtt
     3901 aggcccagcc cctggaaaag ggctcccctt tgacgattcg tgaagcgctg acgttcgacg
     3961 acgttcttct ggttcccgcc gcctctaccg tgctgccggg ccaggtcgat acgcggacgc
     4021 gcctgaccaa aaccatcgag ctcggcattc cgctcatctc ggcggccatg gacacggtaa
     4081 cggaagccgg cctggcaatc gccatggcgc aggccggcgg catgggtgtg atccatcgca
     4141 atctgacccc ggaagaacag gccggtcagg tccggcgggt gaaaaaattc gaatccggca
     4201 tggtcgtgaa ccctctgacc atcggaccgg aagcgacgct tgccgatgcc ctctccctga
     4261 tggagcgcaa ccacatttcg ggcatcccgg tggtggaatc gactggcagg ccagggacgc
     4321 tcgttgggat tctcaccaat cgcgacgtcc gctttgccca ggaccggcgc caatcggtgc
     4381 gcgagctgat gaccaaggag cgccttgtca ccgtgcgcga gaacgtgccg atggaggagg
     4441 ccaagcagct tcttcatcag catcgcatcg aaaagcttct ggtggtggac gagagttacc
     4501 gctgcgtcgg cctgattacg gtcaaggaca tcgaaaaagc gcagaaatac cccaatgcct
     4561 gcaaggatga acgcggccgt ctgcgcgtcg cggccgccac caatgtgggc gaggatgggg
     4621 ttgcgcgcgc catcacgctg ctcgaagcgg aatgcgacgt catcgtggtg gacaccgccc
     4681 acggccattc caagggcgtg cttgagacgg tcgcgcgtat caagcgcgag agcaattatg
     4741 ctcaggtcgt ggccgggaat atcgccaccg ccgatggcgc caaggccctg atcgatgccg
     4801 gcgccgatgc cgtgaaggtc gggatcgggc ccgggtccat ttgcaccacc cgcatcgtgg
     4861 cgggtgtcgg cgtgccgcag ctcaccgccg tgatggatgc cgcggaggcc gcgcgcaaac
     4921 agggtattcc cgtcgtcgcc gacggcggca tcaaatattc gggcgacctc gccaaggcga
     4981 tcgccgccgg cgccgatgtc gcgatgatcg gccaattgct cgccggcacg gatgaaagcc
     5041 cgggcgaggt gttcctgttc caaggtcgtt cctacaaggg ctatcgcggc atggggagcc
     5101 tcggcgcgat ggcacgcggg tcggcggacc gttatttcca ggctgaggtc aaggacacgc
     5161 tcaagcttgt gcctgaaggc gtggagggtc aggtccccta caagggcccc gcgggcgcgg
     5221 tggtccacca gatcgtcggg ggcttgcgtg cggctatggg ttacacaggt tgcgccacta
     5281 tcgatgattt ccacgaaaag gcggaatttg tgcgtatcac cagcgccggc atcaacgaaa
     5341 gtcacgtcca cggcgttgtg gtgacgcggg aaagtcccaa ttacccgcgt ccacaataaa
     5401 ccagcgacgt gccgatgacg acagtgagaa agggccaggc gccgggcccg atgtcccgcg
     5461 atgccttcca cgcgcatttc cggcggtcct tcttcgatcc ggcgttcgat tgcgaaatcg
     5521 atgccattgc ccggctggaa gagatcgcct ggaatgccta ctgcaagggc cgcaaggccc
     5581 cacgcgcgca aaaggcggga gccggcttcg ccgatcctga ctacgatctt tccatcgagt
     5641 ggcatgagac acgcgacgcg ctgatcgtgg ccgaggcgcg gcagaaagac agcaccgcgc
     5701 catcgcgtgt gctcctgatt atcggatcgg cgcgcaatga cggcacctgc cccggcgaaa
     5761 tatccaagag ctttcgcctc gccaatgcgg ccgaggaaat cttcggggcc gctggactca
     5821 atgtggaccg gctcgatctc agccttctgt cgtccgatta cggccggaag attcatccat
     5881 gcaaatcctg cgtcgccacg tcgatggccc tctgccactg gccatgcagt tgctatccca
     5941 accatgcgct cggccaatcc gacgattgga tggcggaaat ctaccaacgc tgggtcgcgg
     6001 cccacggcgt gatgatcgtg acgccggtct attgggggca ggcgccgagc ggcctgaaac
     6061 tgatgatgga ccggctcgtc tgcgccgatg gcggcaatcc cgatccttca tccacccatg
     6121 gcaagaagcc cgaggaagcc aaggcgctgg agctggcggg ctggccttat cccaaacatc
     6181 tcgcgggccg cgtctatggg gtagcggtgc aaggcgatgc ggccggcgtg caggaggtgc
     6241 ggtccgcact ttgcgattgg ctcgattgga acggcttgat cggcgcaggc gcgttatcgc
     6301 agctcgatcg ttatatcggc tatttcgagc cttatgcgac aagccatgaa gcactcgaca
     6361 aggatgcggc ggtcgtcgag gaggtgaaga acgttgcgcg cgccattgca cgcgccattc
     6421 ccgaaatgcg caagggtaag ctcaagccgc cggattctgg cctcaccgaa ccgcgcaaaa
     6481 aataacgcgc tgtatttctt cttgtgggga cgctgaaatt cgggcgtatc tcgccctaat
     6541 gacacctgcc gcacgcattc aggccgccat tgaagtgctc gacgcgctga acgcgaccac
     6601 gatgccggcc gaccggctgt tgcgcgattg gttccgcgcg cgccacttca tgggctccaa
     6661 ggatcgcgcc gccgtagcgg agcgtgtcta tgccgtcctg cgtcatcgcg catcggcggg
     6721 ctggcgcatg ggcgcggagg attcgcgctc gctcgccatc gcaagcctgc ttggcgaggg
     6781 tctgacgggc gatgagatcg cgaaactgtt cgccgccggc gcttacggac cctcgcccct
     6841 cacggagaac gaatcccgcg cgatttccga gccgcccgcg ggtccggcgc cgcttcacgt
     6901 gctcggcgaa tttccccaat ggctggagcc cgaactgacc cgcgccttcg gggaccgtct
     6961 gctggagcat atgcgcgcga tgctgacgcg cgcgccggtg gatttgcgtg tcaacacgct
     7021 acgcgcctcg cgcgccgata tgctggtggg cttgcgttcc ctggatgttg tggcagacct
     7081 cgcacccttc gcacctcacg gaatccgaat cccttcggga gagggccttg gcgcccttca
     7141 gcagacgcag ttcttccaga ccggggcctt tgaattccag gacgagtcct cgcaaatcgc
     7201 cgccgtactc tgcgatgcaa agccgggcag cagcgtgctc gacctcgcag cggggtcggg
     7261 gggcaagtcg ctggcgcttg ccgcggccat gcagaatacc ggcgaaatcc tcgccttcga
     7321 caccgacgcc acacggctga agcagcttcg cccccgcgcg cgccgggccg gcgccacgat
     7381 catcgcggcc accggcaatc tcggtggtcc tttgtggggc gaggagaaat tcgattgtgt
     7441 tttggtggac gccccgtgca gcgggtcggg ggcgtggcgg cggaatccag gggcgaaatg
     7501 gtggctgacg cgggagcggc tcgacagcct catggctctc cagaaccggt tgatcgatga
     7561 cggcgcccgg cacgtcaagc ccggtggccg gctggtctat gccacctgct cggtgctgcc
     7621 ttgtgaaaac gcggatgtga tcgcgcactt cctggcgcgc aatccgggct ttcgggtgct
     7681 cgcggccgag gaaatctggc gttccgccac ggcgagcgcc cccccgccgg gcatgaagga
     7741 ctattttacc gcgaacccgc tgatgactgg catggacggc ttttttgcct gtatcatgag
     7801 caccgactga ggaatttcgc cttgcatgtg tggagaggca tcatgaatcg cagcttgctg
     7861 ggaattatct tggccgccgg tttgacgagt tcggcggcgt gggctgcggc gagcgcgccg
     7921 cagaagcccg cggatgccga aagcgattcc caggccgccc agctgaagcc gcaggacatg
     7981 catttcctca cggatgccga ccaggcggcc aaggccgggg acagcgacgg cgcggtgcag
     8041 ctttaccatt cggccatcat ctatgcaccg ggcgatccgg ttccttacga acggctggcg
     8101 gagttctatg tccatagcaa tcggccggag caggcgcagc gttatttcac gctggcgctc
     8161 gatgcgcagc ccgcctatgc cccggcactt cagggtctgg cgctgctcga cattgccgct
     8221 ggcaatcggg ccgccgccca ggcgcagcat gacatcctgc tgcattcctg cggtgccaaa
     8281 tgcccggaaa cggcgcaggt cgaaaaagct ctgaacagcc gctccgcgcc gtagacaggc
     8341 ccctcttgcc tgactaggtt ccggccatgc ctccggtatt ggtcatcgat ttcggctcgc
     8401 aggtgacgca gctgatcgtg cggcgtgttc gcgaaagcgg cgtctattgc gaagtcgtac
     8461 ccttcaacaa ggccgaagcc gcgcttgccg agggaattcc gggcgccatc atcctgtccg
     8521 gcggcccggc aagcgttggc gatgccggat ctccgcgtgc ccctgaatcg gtgttcacac
     8581 tgggcgttcc ggtgctcggc atctgctatg gccagcaagc catggtcacg caattgggcg
     8641 gccgcgtgga gccgcaccat aagcgcgaat tcggccgtgc cgagatcgaa gtctcgggcc
     8701 agtctccgct tctggagggc gtgtggccgc tcggcagcca tgacaccgtc tggatgagtc
     8761 acggcgaccg ggtgatccag ctccctccgg gctttcatgt gctggcccag agcgacaacg
     8821 caccctttgc ggtcatcgcc gatgaggcgc gcaaattcta tggggtgatg ttccatcccg
     8881 aagtcgtgca cacgcccaag ggcggagcgc tgctgcgcaa tttcacgcaa atcgtggcgg
     8941 gtatcgcgcc cgactggaac atgaaggcgt tccgcgccga agcggtcagg cgcatccggg
     9001 cgcaagtgag cggagcccgc gtcatttgcg gtctctcggg cggcgtggat tcgtcggtcg
     9061 cggcccttct cattcacgag gcggtgggcg atgcgctgac gtgtattttc gtggacaccg
     9121 gattgatgcg cagcggcgaa gcgcaagacg tggtttcgtt gttccgcggc cactacaaca
     9181 ttccccttgt ccatgccgat gcgggcgaat tgttcctgtc gcggctcgaa ggcatcgacg
     9241 acccggagaa gaaacgcaag atcatcggcg cgactttcat cgatgtgttc gacgccgaag
     9301 ccaagaaggt gggacatccg gaatttctcg cgcaaggcac gctctatccc gacgtgatcg
     9361 agagcgtctc ggcgatcggc gggccatcgg tgaccataaa atcgcaccac aatgtcgggg
     9421 gcttgccgga acgcatgcat ctgaagctgg tcgaaccctt gcgcgatctc ttcaaggacg
     9481 aggtgcgcgc gttgggtcgc gagcttggat tgccgcaaag tttcgtcgga cgtcatccct
     9541 tccccggtcc cggtcttgct atccgcattc cgggcgccat cacgccggaa aagctcgata
     9601 tcctgcgccg cgccgatgtc atctatctcg atgaaatccg caaagcgggt ctgtacgacg
     9661 cgatctggca ggccttcgcc gtactgcttc cggtcaagac cgtgggcgtg atgggggatg
     9721 accgcacgta tgaatatgtc tgcggtttgc gcgccgtcac gtcgtcggac ggcatgaccg
     9781 ccgaatccta tccgttcgag cacgcattcc tcgcgcgcgt tgccacccgc attatcaacg
     9841 aagtgcgcgg catcaatcgc gtggtctacg acgtgacgtc gaagcccccc ggcaccatcg
     9901 agtgggaatg attttggcct atccggggct accccacggt gtcccgtaaa cctccataac
     9961 acattgataa acaaaataaa ttcatacaat gacgttcgag gactatcggg cggcagagat
    10021 cgcgcctgac gtacttcctg acggtatgcc tccccattgc aaaagcggat ttcgtccgat
    10081 accgtcaggg tctgagcgcc tctgacggtc tttttttatt cataacttac tgaaagaaaa
    10141 aggatttttg tctagacggt gccaaaaacg gcctgacggt cttttttcgt ggaggcggcc
    10201 aaaatgctga ccgatgcagc ccttaagcat ctgaaaccga aagacaaatt atacaaggtc
    10261 accgaccgcg acggcatgta tgtgcttgtc agaccgtcgg gggtactcac tttccagctc
    10321 gattatcgga tgaacgggcg gcgtgagacc gtcacgttcg gcaaatacgg cccagccgac
    10381 ctgtcgctgg ctcgcgcccg agagctgtgc atcgacgcca aacgggccat cgccgagggg
    10441 cgatcgccgg cgatcgagaa gcagcgggat aagcgccgcc tcctggaagc gaagagcttc
    10501 ggcgagttcg gcgagaaatg gctgaccgcg gcgccgatgg ctgacagcac gcgcgcgatg
    10561 cggcggtcca tcttcgagcg tgagctgctc cccgtgtggc gaaacaggct tctgaaagag
    10621 atcacaccgg acgacctgcg cgcccattgc ggggccatcg tcgagcgtgg cgcgccggcg
    10681 acagctatcc acgtccgcga cattctcaaa cagatctacg gcttcgcgat cctgcacggt
    10741 gaaaaggtcg ccaatccggc cgacgatgtg gggccggcct cgatcgcgac gttcgtgccc
    10801 aaggataggt cgctctcgcc aaccgagatc aggattatgc tcaagctgct cgagaaggtg
    10861 gcgacgctgc cgaccattcg gctcggcatg cgcctctatc tacagaccat ggtgcgcaag
    10921 agcgaattgc aggacgccat ctgggatgag gtcgatttcg agaacgccgt ctggacgatt
    10981 ccgaaggagc ggatgaagcg ttcgaaggcg cacaacgtct acctgtctcg gcagacgcta
    11041 gacattatga tcgcgctgaa aacatgcgcc ggaaattcga gataccttct accgtcgcgt
    11101 tatgacgcgg acgcgccgat gtcgcgcgcg accttcaatc gcgtcaccta tgcggttgtc
    11161 gagcaggcga agaaggaggg cttgccgttg gagccgttca cagttcacga cttgcgaagg
    11221 acgggctcaa cgctgctcaa cgaactgggc ttcaacagcg actggatcga gaaatgcctc
    11281 gcgcatgagg atggccgttc ctcgcgcggc gtttacaaca aggcagagta cgaagtgcag
    11341 cgtcgccata tgatgcagga atggtcggac attatcgacg cctgggtcga gggcaaaaag
    11401 cgcgtgccgg tgctcatccc gccttcgatg ccgctgcttg agcccgaccc ggttctcgaa
    11461 ccgggcgcga tcgccgctgc ctgacgtcag gagcctgcgc gcggactatt ggggctgatc
    11521 ggcgtgaaac cagccaggct tcgacctctg ccaggtccca gacgacgcag cgaggtgaga
    11581 gggcgaaacg ctgcgggaac tcgccgcgct gttccatctc gtagatcgtg ctgtcggcga
    11641 gcggcacgat ctcgcgcaat tgttgcctcc ggatcgtccg tttgattgtc gttgacatgt
    11701 agggcacgct cgcgggttcg agttatcgat acgagacaat tgaaagcgac tgctgcgaaa
    11761 agatggaagt gccgatttca tttcaatggg ccatggaagg ctgccggaca gaaatcggat
    11821 gagcctggat cctctaagtc gaagaatcag gcgatcgatc gcacagcctc gttagacaga
    11881 ttgaggctcg tctcgtggca aaactggcga tctgttcaag aggaggcgag acgttgaagc
    11941 tctactactc aagaaatctg aatccgcgcg tagcggtggc tgttgcgcgc tatttggagc
    12001 tgcctgtgga attcgtccgt gcgagccctc gagaagccaa gaacgaagac gcttttcgtc
    12061 gcatcaatcc gaatgctctg gtgcctgttc tggtcgaaga aaaccggtcg ctttgggaaa
    12121 cggacgcgat agcctgccgg ttggcgatgt tggcaaattc ggacttctag ctcacgggca
    12181 attcggcgcc tgagtttcag atgtgggtga gctggagcac ccatcacttc acgcgcgcgg
    12241 cgagtgtttt ctattgggag tacgtcatca agccgatgat cggcttggga tcggcatccg
    12301 aaagtgcaat tgaagaggca acgggggatt ttcaccgatt tgccggcgtg ctcgacgacg
    12361 ctctgtctgg gcggacgtgg ctggtcggag acaatcccag ctacgccgat tttcgcgttg
    12421 ccacagcttt gccgtttgca gaggcctcga agctgcctct gcgggaatac aagaacatcg
    12481 gcaagtggca tgaccgactc tggcggttcc ctgcctggag cgatccattc gcaggtttag
    12541 cgtagaggat ctgaagatga ctgatcggat actttctgga aaatgttttt gtggcgccgt
    12601 cgctt
//
LOCUS       DASYEG010000049        12831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2913532, whole genome shotgun sequence.
ACCESSION   DASYEG010000049 DASYEG010000000
VERSION     DASYEG010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 12831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12831
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2913532"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2457)
                     /locus_tag="VGM72_01860"
     CDS             complement(<1..2457)
                     /locus_tag="VGM72_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264036.1"
                     /translation="MKCSPRPPLVVTRSGEAIATALAENRRGYTPEWRTPRGMGEAGA
                     ALNDILARYLQIQGDGLNAMPRRLQLELLESLGVNVLPPQAARAPLVFKLVETASGDA
                     TVPMGTRVAAVLPPPSPSLESDAAPKSKSPPEFFTEQEFTAMRGQLGVLYSIDPQADT
                     FVDHTPTASSGFDVFSRMSPVPHRLYLGHGELFKLTGSAQIVLSFDFAASSGGGGSGS
                     QRPLLLDWEYLSVDGWQPLELTEDRTERFTVDGKVTLEKVYGPDSQEDLVGGTKSCWI
                     RATVSNRTPGARISSEAAGYLIRYTPVAPPFSLEPGDRVGITGDPLLVNVLDVGNAIL
                     IVDAPLSAAVRGAPLQRADGTAIGSVLGAPAEFRLPVESTRDLLAGDVVTVDGAQSAS
                     VVTLDGDAVYLSVPLAAAQPGMIIELADALPPLRPDGADDAGVLPQVDLIRARVGFGK
                     TDLPADSAYLDSTSVDISKDFYPFGTEPARFATFYVACKEAFERNGARIELNFTYAQP
                     GRDDGGPKVAAEFFDGSRWIELTINNDYSDGSLSLTRPFETDPAFPHAKLSFTVPANW
                     EASEVNGDKQRWLRLRLSKGDYGHPLALSVEPDPDDNTKYIVKSTPSTLQPPIIARLA
                     VNYLYFTNPQSPDYCLTENDFAFAEHSEDARWPRSPFEPFTPVSDRTPALHFGFTSKP
                     PAALVSLLVQVLAPAIDGDPQPFAWDYWGSRGWTELSVRDATLGLRQTGLLQFVGAPD
                     ALPREGMNGALYQIRARLKTGLASQDQIVQCGGVWLNAAWARQGQSVDRDQLGISNGN
                     PEQTFALPIVRAAKSETVAAP"
     gene            complement(2457..2876)
                     /locus_tag="VGM72_01865"
     CDS             complement(2457..2876)
                     /locus_tag="VGM72_01865"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEY4264037.1"
                     /translation="MAARDTAPDPKAFLGRGLAFPVAVDGTGAVALAEYEEDIRQAIR
                     IILDTDPGERVMRPDFGAGLRALLFEPINTHTLALARHRVEQALILWEPRIDSITVKV
                     DAQPELGILNISIRYRVRTTNTFYNLVYPFYLQEGRD"
     gene            complement(2877..3155)
                     /locus_tag="VGM72_01870"
     CDS             complement(2877..3155)
                     /locus_tag="VGM72_01870"
                     /inference="COORDINATES: protein motif:HMM:NF017315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEY4264038.1"
                     /translation="MPAAARFLDLTNHPGLIVKGSPTVLINGFPAARQTDTHACLLPP
                     LVGPHPSNAISAGSKTVLINGLPAARQLDLAGCGASISTGSPTVLIGG"
     gene            complement(3167..3826)
                     /locus_tag="VGM72_01875"
     CDS             complement(3167..3826)
                     /locus_tag="VGM72_01875"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEY4264039.1"
                     /translation="MANEAVKFGATQQFDSKPPEAKIEGVSIATVLNNIDCTGEARVQ
                     LTLPWLPGYTPWARISSQMAGMASGSFFVPQIGDEVLVAFHHGDVREPYILGTCWNTM
                     DRPPALSPTDAVTKRKIRTPMGHELSFDEATQSVTLSSNTMSTVTLDLEKAEISTPLA
                     KVTIGKAGNVTISAKTMLTLEAPVIKINAKTVLTAQSGGVAQFKAGGVCSVQGATVKI
                     N"
     gene            complement(3854..5050)
                     /locus_tag="VGM72_01880"
     CDS             complement(3854..5050)
                     /locus_tag="VGM72_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264040.1"
                     /translation="MSNTRFAPEFALMLDGKSIPAELRASILSVRCQTGYEGLDEVEI
                     TLANEKLRWLDDPMFKLDTSLTLKLGYAPDPLVQTFDGEVVARAASFPSGSIPTFSIT
                     AHDRRHKMRDGKKVRWFAIPLPCPGNLPLPDIATASIVTLENLMLPIFDPVGAALSII
                     LGGVDTFVAITDPYSAQKVIRKQVNESDYDFLGRIATENGWDVLVEHDGAVGGHLLRF
                     TSSLDHLDADFTFGYGRSLIDFAPRISQVGQIFTVGGFVWIPAIKTTFTVTLGFDWDR
                     MSLTLSVSPGAIPIGMQPGDYMVDEPLTPTSIPRKLVSELIPRLNRRLTATGTVIGDP
                     NIRAGNVIRIEGTGEEFGGLYRATAVTHTIDGGGFRTQFEGRKEIWFGSIPLADQGAV
                     PIRVSF"
     gene            complement(5060..5746)
                     /locus_tag="VGM72_01885"
     CDS             complement(5060..5746)
                     /locus_tag="VGM72_01885"
                     /inference="COORDINATES: protein motif:HMM:NF039279.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264041.1"
                     /translation="MALEKALLTNTVTGMKIPVQFNPEEYTLNRDINYAQAAIPGLSA
                     PILQFVNGNLQTLEMELFLDSYEKHKVGSTTINEAQSDVRVLVKSVADLMSIDPTTHA
                     PPVLLFTWGSLAFTCVLARLVQRFLMFLPDGIPVRARLNVTFNEYRNIDLEAKEVKRE
                     TSDYSKRRVVIEGETLSSIAGVEYGDPRLWRVIAIANRLQRARNLEPGSKLLLPNLPY
                     RDPDSGRIYA"
     gene            complement(5756..6421)
                     /locus_tag="VGM72_01890"
     CDS             complement(5756..6421)
                     /locus_tag="VGM72_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264042.1"
                     /translation="MIFTAMLARHKVQSAYSATAARHRAFVTRVFGVFHVKGGMSPVG
                     RVMRRALGARFYQTMLHVQLALAAVTRNNSQTHGGARRIGQQRHRTIPSRTSVRQIER
                     LQSWRTRVVAPWDTMPQRAYRLAPQIAGRGHVERNVLPPKVAMTVIRERVQVTSQPVL
                     SPRIETSPQPAARSASTIAVPHGIDKKSFSLPVQELSRITDHVIQQLDRRILSYRERS
                     GRM"
     gene            complement(6556..6951)
                     /locus_tag="VGM72_01895"
     CDS             complement(6556..6951)
                     /locus_tag="VGM72_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264043.1"
                     /translation="MFQTEFEFSLPCGFLDEDGTLHKEGVMRRATAADEILPLKDPRV
                     VKNEAYVIVILLSRVVTRLGGLGQVNPKVVEGLFATDLAFLQELYNKINSYDRTEACP
                     HCGKPLHGVGSMGAEAPRPGESSATPSIN"
     gene            complement(6980..7414)
                     /locus_tag="VGM72_01900"
     CDS             complement(6980..7414)
                     /locus_tag="VGM72_01900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEY4264044.1"
                     /translation="MATRKDPYVNFNFLVEIDGITRAAFHEAGGLDSSVDVIEHREGG
                     ENITMRKLPGQVKFSSISLKWGMTDDVDLYKWHRQWVDGDPAAPRKNGSIVLLDRQGQ
                     EKARWNFFNAWPTKWTGPSFNAEANDVAIESLEIAHEGLARA"
     gene            complement(7429..9399)
                     /locus_tag="VGM72_01905"
     CDS             complement(7429..9399)
                     /locus_tag="VGM72_01905"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEY4264045.1"
                     /translation="MANYFTPEVYIEEFEPAAPIAGVGTSTAAFLGPCAQGPLNQPTR
                     VNSWDEFVRTFGAAPVPNFYLWYAARGFYENGGTTAFITRVSNATYGEMTLNDISAAP
                     TLIVRARQAGLLNPGFSITVDSNIHTVDAPARLFRPTATIAGAAVNTRIITLTSAADA
                     ERFRPGDRITWAGVAPADDRATIARVNGATLEILNPLSAGYGGGALRLANPAAGDDTL
                     RVENGAAVAPGMVLSIKQAAPAAVANTMAVKSVTIERISGALTTHRVTFQSGLTAAYD
                     FSGGQVDVQSQEFKLTVTPAVGTPIVYDGLGMDPAHPRYYDTIVNGVDPYIMLIPVDP
                     VNTTPVPNNRPNTLPIAAMANGTADTPATLTSANYKSAIAQLEAIDDINIISIPDRVD
                     PDVQTALVGHCEKMADRFAVLDAARNLPPFGTPSINTQRSGLTSSRGYAALYYPWLTV
                     SDNTGNLTLNVPPSGHMAGIYARTDQLRGVHKAPAGTEAIVRGALGVERAITHTEQEG
                     LNPNGINVVRVFSAGGRPIVWGARTTASGYGGDANWQYVNIRRLFLYLEESIQEGIRG
                     AVFEPNNLALWQKLKRSIGDFLTRAWRDGALFGAKAEEAFYVRIDEVLNPFSEQQLGR
                     LHIEIGVRPSYPAEFIIVRIGIWDGGSEVTEA"
     gene            complement(9409..10326)
                     /locus_tag="VGM72_01910"
     CDS             complement(9409..10326)
                     /locus_tag="VGM72_01910"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pvc16 family protein"
                     /protein_id="HEY4264046.1"
                     /translation="MIDHLDNLLHRLFRYSIAELSADAQIRFQPPDQDWRGIVTTITD
                     GAGNPGNSLNVYLIDLRENRRLRSNEREKMVVGQDIFEIPPARRVDCHYLISAWSPVT
                     VTIGIDPTFDEHALLAETARVLGAHDELDPVAIYALSNPPAPPPPVIADERFPLTLLP
                     VEGFPKFAEFWGTMGDKNRWKPCVYTVITVPLKETALPAGPMVTTAMMDTRLWGVPVT
                     ANTLMQFGGTIRASAVPNAPPVAGAWVDLLTTTGARRQLVRTDINGRFLFVQVAAGTY
                     QIRVSATGFGSFVNPIDVPAPAGSYDMHF"
     gene            complement(10337..12679)
                     /locus_tag="VGM72_01915"
     CDS             complement(10337..12679)
                     /locus_tag="VGM72_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264047.1"
                     /translation="MTDGLGSSLLHSGEKLATEESGAAEDLKGFGRKKAQQGIGAAKA
                     VATQVTEVVDTGLWLGGEYKGLRDKAATSIGGKKGSPGNIAVTQLIDTTARIIPGLAA
                     LPAAAEAGDQLKALGLVDPDTKDEKSGEAQASLTAPLAAKLSTAVAWTDEHIGGTPSD
                     PDLFTETEKAELATNVGVQVLLAFTGAEEVKIAMNVAGALGGLRGVVESIRREPDWKT
                     SSRFWSSVIGLVLSVAGLKHTLAATKITTLLLKFGWVAAAVPPLAQMLADYVNPNLAE
                     STDLPAEENERRRNELNRRMKQNWTQVVHVLKDAILHVAQSQGGKSAAPNEPPASAQK
                     NTGGLPEGGTIQPPPKTPAPPIESKSATAVTTEPVSPAKPVNEGNVTPIKGGPARQKS
                     EASQKLESALSKQSPEKAGSVTPLRPRKGGKPPSEPQQQAVPAVEEQALPVAVGQTHG
                     PEGGGNRPQLRAVDNNEPIVGVRKSATSAVKKSAAAPGTKSTSSGGAPSRSRSTSGRP
                     GTKAASGRGGTKAPAKKQPSKSGGKAGAAQPKLVAVRVQGGGAKRKGAYFEEGTPEFD
                     KAAKDKRFIVVPKSEADPRSLDPKSPDRGLGFRPAGTPHDTGIQPVKINGVQVESNGA
                     GGGKGNKHTISARADTQRGRAAAKKVFEKTIRATIAESHGYKQLLTDGELGIQRAGNV
                     STGGVDAITARVQGGKAKIFLNDFTGPKTSKGAKATHQNWVGELQTALSDKNFGFGNK
                     ATDRAIKSALRRGEVYVRTVRVEYGAGGVKVTVGRPVKVQ"
BASE COUNT         2303 a         3886 c         4021 g         2621 t
ORIGIN      
        1 cggcgcggcg accgtctccg acttcgctgc cctcacaatg ggcagggcaa atgtctgttc
       61 cggattgccg ttgctgatgc ccagttgatc gcgatccacg ctctgtccct ggcgggccca
      121 ggccgcgttc agccatacac cgccgcattg aacgatctgg tcctggctcg caagcccggt
      181 cttcaaccgc gcgcgaatct ggtacagcgc accattcata ccctcgcgtg gcagcgcatc
      241 gggcgcgccg acgaattgca gtagcccggt ctgccggagc cccaacgtgg cgtcgcggac
      301 cgacagctct gtccagcccc ggctccccca gtagtcccat gcgaaaggct gtggatcgcc
      361 gtcgatggcg ggcgcgagca cttggacgag caagctgacc aacgctgccg gaggcttgct
      421 cgtgaagcca aaatgcagcg ccggcgtgcg gtccgacacc ggcgtaaacg gctcaaacgg
      481 gctgcgtggc cagcgtgcat cctcgctgtg ctcggcgaat gcgaagtcgt tctcggtcag
      541 gcaataatcg ggtgattggg ggttggtgaa atataaatag ttgaccgcca gccgcgcaat
      601 gatgggcggc tgcagggtgg agggggtgct ctttacgata tattttgtgt tgtcatcggg
      661 gtccggctcg accgagaggg ccagcggatg cccatagtca cccttgctga gccgcagccg
      721 cagccaccgc tgcttgtcac cgttgacttc agacgcttcc caattcgcgg gaacggtgaa
      781 cgacagcttg gcgtgcggaa atgcgggatc ggtctcgaag ggccgcgtca gcgagaggct
      841 gccatccgaa tagtcgttgt tgatcgtcag ctcgatccaa cgggagccgt cgaagaactc
      901 cgcggcgacc tttggcccgc catcgtcgcg acctggttgc gcataggtga aattgagctc
      961 gatgcgggct ccgttgcgct cgaatgcttc cttgcaagct acatagaaag tggcaaagcg
     1021 tgccggctcc gttccgaaag gatagaaatc cttgctgata tcgacactgg tgctgtcgag
     1081 ataggcgctg tcggcgggca ggtccgtctt gccgaagcca acacgtgccc gtatcagatc
     1141 gacctgcggc aagactcccg cgtcgtcagc gccatcgggg cgcagtggcg gcagggcatc
     1201 cgccagttcg atgatcattc cgggctgcgc cgcggccagc ggaacgctca gataaactgc
     1261 gtcgccatcc aatgttacga cgctcgcgct ctgcgctccg tccacggtca cgacgtcgcc
     1321 ggccaacaga tctcgcgtgc tttccaccgg cagccgaaat tcggcgggcg caccgagcac
     1381 gctcccgatg gcggtgccgt cggctctctg cagcggcgct ccccggaccg ccgcggacag
     1441 cggagcatcg acgatcaaga tggcgttgcc cacatccagc acgttaacca gcagcggatc
     1501 gccggtgatg ccaacccgat ctcctggttc caatgaaaac gggggagcga ccggggtgta
     1561 gcgaatcaga taaccggcgg cttcgctgct gatgcgtgca cctggcgtcc gattcgacac
     1621 ggtggcgcgg atccagcaac tcttagtgcc gccgacgagg tcctcctggc tgtcgggacc
     1681 gtagaccttt tccagggtta ccttcccatc caccgtgaac cgttcggtgc gatcctcggt
     1741 caattccagc ggctgccaac cgtcgacgct caggtactcc cagtccaaca gcaatggtcg
     1801 ctgtgaacca gatccaccgc cgccgctcga agccgcgaag tcgaaggaca atacgatctg
     1861 cgcagagccg gtcaatttga agagctcgcc atgccccaga tagaggcgat ggggcaccgg
     1921 cgacatgcgg gagaagacat cgaatccgga agatgcggtg ggggtatggt cgacaaaggt
     1981 gtctgcctgc ggatcgatgg agtataagac acccagctgg ccacgcatgg ccgtgaattc
     2041 ctgctcggtg aaaaactcag gcggcgactt gctcttcggc gccgcatcgc tctccaaaga
     2101 aggggatggc ggaggcagta cagcggcgac gcgcgtgccc atgggaacgg ttgcgtcacc
     2161 cgaggccgtt tccaccagct tgaacaccag aggcgcacgt gccgcttgcg gcggaagaac
     2221 gttcacgccc agcgattcaa gaagctccaa ttgcagccgc cgcggcatcg cgttcaggcc
     2281 gtcgccctga atttggagat agcgggccag gatatcgttc aacgcggcgc cggcttcgcc
     2341 catgccgcgc ggtgtgcgcc attccggtgt gtaaccgcgg cggttctctg caagcgctgt
     2401 cgcgatggct tccccgcttc gggtgacgac cagtggaggg cgcggcgagc atttcatcag
     2461 tcgcgcccct cctgcagata gaacgggtag acaaggttgt agaaggtgtt ggtggtgcgc
     2521 acacggtatc ggatgctgat gttcagaatg ccgagttccg gctgtgcgtc gaccttcaca
     2581 gtgatgctgt cgatgcgcgg ctcccacagg atcagtgcct gttcgacgcg atgccgcgcc
     2641 aatgcgagcg tgtgggtgtt gatgggctca aacaagagcg cgcgcaggcc ggcgccaaag
     2701 tcggggcgca tcacacgctc gcccggatcg gtatcgagga tgatacggat cgcctgtcgg
     2761 atgtcttctt catactcggc cagagcaacg gcacccgtgc cgtccacggc aaccgggaag
     2821 gcgaggccac gcccgagaaa ggccttggga tccggcgctg tatccctcgc ggccattcag
     2881 cctccgatca gcacggtggg cgagcctgtg gagatgctgg caccgcatcc ggcgagatcg
     2941 agctggcgcg cggccggcaa gccgttgatc agcacggtct tgctgccggc gctgatcgcg
     3001 ttcgagggat gcggtcccac cagcggcggc aggaggcagg catgcgtatc cgtctggcgc
     3061 gctgcgggaa atccattgat cagcaccgta ggagagccct taacgatcaa tccgggatga
     3121 ttggtgagat ctaagaaccg ggcggctgca ggcatcggat atcctttcag ttgattttca
     3181 ccgtggcgcc ttggacggaa cacacgccac cggccttgaa ttgggcaaca ccgccgctct
     3241 gcgcggtcag cacggtcttc gcattgatct tgatgaccgg ggcttcgagc gtgagcatgg
     3301 tcttcgcact gatggtcacg ttgcccgctt ttccgatggt gaccttggcg agcggcgtgc
     3361 tgatttcggc tttttccaga tcgagcgtca ctgtcgacat ggtattcgac gacagagtca
     3421 ccgattgggt ggcttcgtcg aaagacagct catgacccat cggcgtgcgg atcttacgtt
     3481 tggtgactgc gtccgtgggc gacagcgcag gcggacggtc catcgtgttc cagcaggtgc
     3541 cgaggatgta aggttcgcgc acgtcgccat gatggaacgc caccagcact tcatcgccga
     3601 tctgcggcac gaagaacgag ccgctcgcca tgccggccat ctgcgacgag atgcgcgccc
     3661 atggcgtata gccgggcagc cagggcaacg tcagctgcac gcgtgcttcg ccggtacaat
     3721 cgatgttgtt cagtaccgtc gcgatgctga cgccttcgat cttggcctca ggaggcttgg
     3781 aatcgaattg ctgtgtcgca ccgaatttca ctgcttcatt ggccatcttg cgtcccttgc
     3841 ttcccccgtc tgatcagaac gaaacgcgta tcggcacggc gccctgatcg gccaagggga
     3901 tggagccgaa ccagatttcc ttgcgtccct cgaattgcgt gcgaaagccg ccgccgtcga
     3961 tcgtgtgggt cacggcggtg gcgcgataaa gcccaccgaa ctcctcgccg gttccctcta
     4021 tccggatgac gttgccggcc cgtatgttgg ggtcgccgat gaccgtgccg gtcgcggtca
     4081 ggcgccgatt gagccttggt atcaactcgg aaaccagctt ccgcggaatc gatgtcggcg
     4141 tgaggggctc gtcgaccatg tagtcgccgg gctgcatccc gatgggaatc gctccgggcg
     4201 acaccgaaag ggtcaggctc atgcggtccc aatcaaagcc cagagtgact gtgaatgtcg
     4261 tcttgatggc ggggatccaa acgaaaccac cgacagtgaa gatctggccg acctgggaga
     4321 tccgtggcgc gaagtctatc agggagcggc catagccgaa cgtgaaatcc gcatccaaat
     4381 ggtcgagcga ggacgtgaat cgcagcagat gcccgcccac cgcgccgtca tgctcgacca
     4441 gcacgtccca gccgttctcg gttgcgatgc gacccaggaa atcatagtcg ctctcgttca
     4501 cctgcttgcg tatgactttc tgcgccgaat atggatcggt gatagcaacg aaggtatcga
     4561 cgccgcccaa aatgatggac agagctgccc caaccggatc gaagatcggc aacatgaggt
     4621 tttccagcgt gacgatgctg gccgtcgcga tatcggggag cggaagattc ccggggcagg
     4681 gcaggggaat ggcgaaccag cgcaccttct tgccatccct cattttgtgc cggcgatcat
     4741 gggccgtgat gctgaatgtc ggaatactgc ccgagggaaa gcttgcggcg cgggcgacca
     4801 cctcgccgtc aaatgtctgc accaggggat cgggtgcgta gccaagcttc aatgtcagcg
     4861 aggtgtcgag cttgaacatg ggatcgtcca accagcgcag cttctcgttc gccagtgtta
     4921 tctcgacctc gtccagccct tcgtagcccg tctggcaacg cacgctgagg atcgacgcac
     4981 gcagctctgc cgggatcgac tttccgtcga gcatcagcgc gaattccggc gcgaagcgtg
     5041 tattgctcat gatttcgacc tacgcataga ttcttcccga atcggggtca cggtagggaa
     5101 ggtttggcag cagcagcttc gagccgggct cgaggttgcg tgcgcgctgc aggcggttgg
     5161 cgattgcgat gacacgccag aggcgcggat cgccgtattc cacaccggca atcgaggaca
     5221 gcgtctctcc ctcgatgacc acgcggcgct tggaatagtc gctggtctcc cgtttgacct
     5281 ctttcgcctc gagatcgatg ttgcggtatt cgttgaatgt gacgttcaag cgcgcgcgca
     5341 ccgggatgcc gtcgggcagg aacatcagaa accgctggac aaggcgcgca aggacgcagg
     5401 tgaatgcaag cgatccccag gtgaacagca gcaccggcgg agcatgcgtc gtgggatcga
     5461 tggacatcaa atccgcgacc gatttgacca atacccgcac gtccgactga gcctcattga
     5521 tcgtagtgct acccactttg tgtttctcat aggaatccag gaacagttcc atctcgagcg
     5581 tctgcagatt gccattgacg aactgaagaa ttggcgctga gaggcctggg atagcggcct
     5641 gcgcgtagtt gatgtcgcgg ttgagcgtgt attcctcggg attgaactgc acggggatct
     5701 tcattcccgt aacggtgttc gtcagcaatg ccttttccaa tgccatcggg cgcctctaca
     5761 tccgtccgga gcgttcgcgg tacgacagaa tccgacggtc caattgctga atgacgtgat
     5821 cggtgatgcg ggagagttct tgaaccggca gcgagaaact tttcttgtcg atcccatgcg
     5881 ggaccgcaat ggtgcttgcc gatcgtgctg ccggctgagg cgaggtttca attcgaggcg
     5941 acaggaccgg ctgcgaggta acctgtaccc gctcgcgaat aacggtcatg gccaccttgg
     6001 gcgggaggac gttgcgctca acatgtccac gcccggcgat ttgcggtgcc aggcgataag
     6061 cacgctgggg cattgtgtcc cagggagcaa ccacgcgtgt gcgccatgac tgcagccgtt
     6121 cgatttggcg gacggacgtt cggctgggta tggtgcggtg cctttgctgg ccaattcgtc
     6181 tggcgccacc gtgagtttgc gaattgttgc gtgtgacggc ggcgagcgcc agctgtacgt
     6241 ggagcatcgt ctgatagaaa cgggcgccaa gggctcgacg catcacccgc ccaaccgggc
     6301 tcattccgcc tttaacatga aacaccccga agacccgcgt gacgaaagct cgatggcgcg
     6361 cggccgtggc gctataggca gattggactt tgtgacgggc aagcatcgcg gtgaaaatca
     6421 cgctctattc tccgttgctc cgctcattca gcgccgagat ctggctgacc cactgcctgc
     6481 gatcgtcatg ctccatctcc atgatctgat cgtgcggcca gtgaaaatga tatgcgatga
     6541 acgccacctc ctcggtcaat tgatcgaggg ggtagccgat gattcccccg ggcggggcgc
     6601 ctccgcgccc atcgaaccga ctccatgcag gggtttgccg caatgcgggc aggcctcggt
     6661 gcgatcatag gaattgatct tgttgtacag ttcctgcagg aaggcgagat ccgtggcgaa
     6721 tagaccctcg accaccttcg ggttcacctg tccgagaccc ccgagccgcg taacgacgcg
     6781 cgacagcagg atgacgatga cataggcctc gttcttgacc acgcggggat ccttcagcgg
     6841 cagaatctcg tccgcggcgg tggcgcgtcg catgacacct tctttgtgaa gcgtgccgtc
     6901 ctcatcgagg aatccgcagg gcagggagaa ttcaaattcg gtctgaaaca tggctcggct
     6961 ccttggggtt aacgcggcct cacgcccgcg ccaggccttc atgcgcgatt tccagcgatt
     7021 cgatggcgac gtcgttcgcc tcggcgttga agctcggccc cgtccacttg gtcggccagg
     7081 cgttgaagaa gttccagcgc gccttctcct ggccttgccg gtcgagcaat acgatggagc
     7141 cgttcttgcg cggcgccgcg gggtcgccgt caacccactg gcggtgccat ttgtacaggt
     7201 cgacgtcgtc ggtcatgccc catttgaggc tgatgctcga gaacttcacc tggccgggca
     7261 gtttgcgcat ggttatgttc tcgccgcctt cgcggtgctc gattacgtcc acagaagaat
     7321 cgagacctcc cgcttcgtga aaggccgcgc gcgtgatgcc gtcgatctcg accaggaagt
     7381 tgaaattgac gtaaggatct ttgcgggtcg ccatcgtcgt gctcctgtct aagcttcggt
     7441 gacttccgag ccaccatccc agatgccgat gcgaacaatg atgaattctg cggggtagct
     7501 cggccgcacg ccgatctcga tgtgcaagcg gcccaactgc tgctcggaga acgggttgag
     7561 cacctcatcg atgcgcacgt agaacgcctc ttccgccttt gccccgaaca gagctccgtc
     7621 acgccaagca cgcgtgagga agtcgccgat ggaccgcttg agtttttgcc acaatgcgag
     7681 attgttcggc tcgaacaccg ccccgcgaat gccttcttga atcgactctt cgagatagag
     7741 gaacaggcgg cggatgttca cgtattgcca attcgcatcg ccgccatagc cgctggcggt
     7801 ggtgcgggca ccccaaacga tcggccgccc gcccgcggaa aatacgcgaa cgacgttgat
     7861 gccgttcgga ttgaggcctt cctgttccgt atgcgtgatc gcgcgctcga caccgagcgc
     7921 gccgcggacg atggcttccg tgccagccgg cgctttatgt accccgcgca attgatccgt
     7981 gcgtgcgtag atgccggcca tatggccgga cggcggcacg ttgagagtga gattgcccgt
     8041 gttgtcgctg acggtgagcc atgggtaata cagcgccgca tagccacgag aggacgtaag
     8101 gccgctccgt tgcgtgttta tgcttggtgt cccgaacggc ggcaggttgc gcgccgcgtc
     8161 caagaccgcg aaccgatccg ccattttctc gcaatgcccg accaaagcag tctggacgtc
     8221 gggatccacg cgatccggga tcgagattat gttgatgtca tcgatggcct cgagctgcgc
     8281 gatggcgctt ttgtagttgg cggaggtcaa ggtcgcgggc gtgtcggccg tcccgttggc
     8341 catcgctgcg ataggcaatg tgttcggccg gttgttggga accggcgtgg tgttcaccgg
     8401 gtccaccgga atcaacatga tatagggatc gacgccgttg acgatggtgt cgtaatagcg
     8461 aggatgcgcc gggtccattc ccaagccgtc atagacaatc ggagttccaa ccgcgggcgt
     8521 caccgtcagc ttgaattcct gcgactgcac atccacctgg ccgccggaaa agtcataggc
     8581 agcagtcagg ccgctttgaa aggtgacgcg atgcgtggtg agcgcacctg aaatgcgttc
     8641 gatggtcacc gatttcacgg ccatcgtatt ggcgaccgcg gcgggcgccg cctgcttgat
     8701 cgacaagacc atgccggggg ccactgcggc accattctcg acacgcagcg tgtcatcacc
     8761 cgccgcggga ttggcgaggc gaagggcgcc gccaccatat ccagccgata ggggattcaa
     8821 gatctccagc gttgcaccgt tgacgcgggc tatggttgca cgatcatcgg cgggggctac
     8881 gcccgcccaa gtgatgcgat ctcctgggcg gaatcgctcg gcatccgcgg ccgaggtgag
     8941 ggtgatgatc ctggtattga ccgccgcgcc cgcgatcgtc gctgtgggac ggaaaagtcg
     9001 cgccggcgca tcgacagtgt ggatattgct atcaacggtg atgctgaaac ctggattgag
     9061 caggccggcc tggcgtgcac gcacgatcag cgtcggcgca gcgctgatgt cgttcagcgt
     9121 catctcgcca taagtcgcgt tgctgacgcg cgtgatgaac gccgtcgtgc cgccgttttc
     9181 gtagaagccg cgggcagcat accaaaggta gaaattcggc acaggtgccg cgccaaacgt
     9241 gcgaacgaac tcgtcccagc tgttcacacg ggtgggctgg ttgaggggtc cctgggcaca
     9301 gggccccagg aaggccgcgg tgctggtgcc gacgccggca atcggagctg caggttcgaa
     9361 ttcttcaata taaacctcag gagtaaaata gttcgccatg ggcctctcct aaaaatgcat
     9421 gtcataactg ccggcgggag cgggcacgtc gatcggattg acgaaactgc caaatcccgt
     9481 tgcggagact ctgatctggt acgtgccggc cgcgacctga acgaacagaa agcggccgtt
     9541 gatatcggta cgtaccagct ggcggcgcgc acccgttgtg gtcagcagat ccacccacgc
     9601 accggcgaca ggcggtgcat tcggtactgc gctcgcccgt atcgttcccc caaactgcat
     9661 cagcgtattc gcggtcaccg gcacgcccca caggcgcgta tccatcatcg ccgtggtgac
     9721 catggggccg gcgggcaggg cggtctcttt cagcggaacg gtaatcaccg tatagacgca
     9781 cggcttccag cgattcttgt cgcccatcgt cccccagaat tccgcgaatt tcgggaaacc
     9841 ctcgaccggc aacaacgtca aggggaagcg ttcatccgcg atcacggggg gtggaggagc
     9901 ggggggattg gagagtgcat atatcgcgac gggatcgagc tcgtcatggg cgcccagcac
     9961 gcgcgcggtc tcggcgagca atgcatgctc gtcgaaggtc ggatcgatgc cgatcgtaac
    10021 cgtcaccggg ctccatgcgc tgatcagata gtggcaatcg acgcgacgcg cgggcgggat
    10081 ttcgaaaata tcctgtccca ccaccatctt ctcacgctcg ttcgatctca ggcggcgatt
    10141 ctctcgtagg tcaatgaggt agacgttcag cgaattccca ggattgcccg cgccatcggt
    10201 gatggtggtg acgatgccgc gccaatcctg gtccggcggc tggaaccgaa tctgcgcatc
    10261 ggcgctcagt tccgcgatcg agtagcggaa caggcggtgc aacaaattat cgaggtgatc
    10321 gatcatccgg cgcctctcat tggactttca ccggtcgacc gacagtgacc ttcacacccc
    10381 cagcgccata ttcgacgcgg acggtgcgca catagacttc accgcgtcgc agtgcgctct
    10441 tgatcgccct gtctgtggcc ttgttgccga agccgaaatt cttgtcgctt aacgcggttt
    10501 gcagctcacc gacccagttc tggtgggtgg cttttgcgcc cttagaggtt tttgggccgg
    10561 tgaaatcgtt cagaaatatc ttggcttttc cgccttgcac acgagcggtt attgcatcaa
    10621 cgcccccggt gctgacattg ccagcgcgct gaattccgag ctcgccatca gtaagcagct
    10681 gtttatagcc gtgcgattcg gcaatggtcg ctctgatagt cttttcgaag acttttttcg
    10741 cagcggcgcg gcctctttgg gtgtcggcgc gtgccgagat cgtatgtttg ttacccttgc
    10801 cgcctccggc accattggat tcgacctgca cgccgttgat cttgaccggt tgaatgccgg
    10861 tatcatgtgg cgtgcccgct ggccgaaagc ccaggccgcg atccgggctt ttcggatcaa
    10921 ggcttctcgg gtcggcctcg ctcttcggca ccacaatgaa gcgcttgtct ttggcggcct
    10981 tatcgaattc cggcgtaccc tcttcgaaat aggcgccttt acgcttcgct ccgccacctt
    11041 gtactcggac tgcgacgagc ttaggttgcg cggcgcccgc cttgcctccg gacttgctcg
    11101 gttgtttttt cgccggcgcc ttggtgccgc ctctacctga ggcggccttc gtacccggcc
    11161 taccgctcgt agagcgactg cgggatggtg cgccgccaga ggatgtgctc ttggtgcctg
    11221 gggctgcagc ggatttcttg actgctgatg tcgcgctctt ccgcacaccg acgatcggct
    11281 cgttgttgtc caccgcgcgc aattgcggcc gattgccgcc gccttccggg ccatgggttt
    11341 gtccgacggc gactggaagc gcctgctctt ccactgctgg aactgcctgc tgttgcggtt
    11401 cgctcggcgg ctttccgccc ttgcgcggac gcaagggtgt gacgcttccc gccttctccg
    11461 gggattgctt ggaaagcgcg ctttccagct tttgcgaggc ctcggatttc tggcgcgcag
    11521 gcccaccctt tatcggcgtg acattgccct cgttcaccgg tttcgcggga ctgacgggct
    11581 ccgtggtgac ggcggttgcc gatttggact cgatcggggg cgccggcgtc tttggcggcg
    11641 gttggattgt gccgccttcc ggaagaccgc ccgtgttctt ttgagcggag gcaggtggtt
    11701 cgttgggggc cgccgacttg ccgccctgcg actgcgccac gtgcaggatc gcgtccttga
    11761 ggacgtggac gacttgcgtc cagttctgct tcatgcggcg attgagctca ttgcggcggc
    11821 gctcattctc ttcggcgggc aaatcggtgc tctcggcgag attggggttc acatagtcgg
    11881 caagcatttg cgcaagtgga ggaacggcgg ccgcaaccca tccaaacttg agaagcaatg
    11941 tggttatttt tgtcgcggcc agtgtgtgct taagccccgc cacggagagc acgagcccga
    12001 tcacgctgga ccagaagcga gagcttgttt tccagtccgg ttcgcgccga atgctttcga
    12061 cgacgccgcg caggccaccc agcgctccag cgacgttcat cgctatcttg acttcttccg
    12121 cgccggtgaa agccagcaga acctgtacgc cgacattggt ggcgagctct gccttttcgg
    12181 tttccgtgaa gagatccggg tcggagggcg tgccgccgat atgttcgtcg gtccacgcca
    12241 cggcggtact gagcttcgcc gccaggggcg ccgtcaggct cgcctgcgct tcgccggact
    12301 tctcgtcctt ggtgtccggg tcgaccagac ccagggcttt caattgatct cccgcttctg
    12361 cagcggccgg gagcgcagcc aggcccggga tgattctcgc cgtggtgtcg atcagttggg
    12421 tgaccgcgat gtttcccggg ctgccttttt tgccgccgat ggatgtggcg gccttatcgc
    12481 gcagtccttt gtattcaccg cccagccaca accccgtatc gacgacctcg gttacctggg
    12541 tcgccaccgc cttggcggcg ccgatgccct gctgcgcctt cttgcggccg aagcctttga
    12601 ggtcctctgc ggctccgctt tcttcggttg caagcttttc gccgctgtgc aacagcgaag
    12661 aaccgagacc atcggtcacg atcggtggcg cgataggact cagcaccacg ctgacggtct
    12721 tatccgccac gacatcggcg actttggcaa tcggttcgcg gtatctctcg ggaattctct
    12781 ccttcaacgc gttgacacgg cgcgtaaagc gtgcgcccca gctgtcatcg t
//
LOCUS       DASYEG010000050        21920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4364604, whole genome shotgun sequence.
ACCESSION   DASYEG010000050 DASYEG010000000
VERSION     DASYEG010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 21920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21920
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4364604"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VGM72_01920"
     CDS             <1..425
                     /locus_tag="VGM72_01920"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY4264048.1"
                     /translation="TSLGIGISLAQTIFDGGRLTGESDEARAREQELIANYRNTAIVA
                     FSEVETSLGGIAHFRELEVYQAEQVAQSQIAFDIAQVRYREGVADYLTVLDAQRTLYS
                     ARDSLGQIKLQRLQAIVALYKALGGGWTEPSSSTLARD"
     gene            complement(429..1121)
                     /locus_tag="VGM72_01925"
     CDS             complement(429..1121)
                     /locus_tag="VGM72_01925"
                     /inference="COORDINATES: protein motif:HMM:NF018482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEY4264049.1"
                     /translation="MFRNLAGFALAGALLAILPGGAIAAQQAAAAPPPEAPPAAPPAP
                     PQYSINQSVGDWVVRCVQTTVKSPAPCEVMQTTVNKDNKQRISAFSLAYVPSRDAYAV
                     QVVVPTGVALSKGLQLGTALNGAKFNRCERDGCYVEMLLDNAAVASISGAGKSTTIGV
                     VGYGQANEIKLPVSLTGFPEALDRMKGYAKDRAVALPAGANPLPVAPPPTLPGPVAGR
                     AAPAPAKPAGGK"
     gene            complement(1150..1779)
                     /locus_tag="VGM72_01930"
     CDS             complement(1150..1779)
                     /locus_tag="VGM72_01930"
                     /inference="COORDINATES: protein motif:HMM:NF018482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEY4264050.1"
                     /translation="MTETSNQRVRAGTRSMDGGRWGRWALRGGIVILLLAAGGVIALA
                     AERLLGGIAPNEMRISTFQDWRVICPPLTKTTPNCALTSDVLRDTGGTLLTLSVLDPA
                     PGSQLSLTVPHGVLLDPGLGFAIGTEPTRVRPYETCSNQGCIALVTMDADTLKALDAN
                     MGGHVVVAAPNNPQPINIPYSLKGFADGFGELQRAKARRTGMLSFLNRS"
     gene            complement(2003..4084)
                     /locus_tag="VGM72_01935"
     CDS             complement(2003..4084)
                     /locus_tag="VGM72_01935"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEY4264051.1"
                     /translation="MKRTGLAVLGILLTISAMGAIAAQTAPVSNQATAIAKKPTFGNW
                     GVDLSGMDKTVAPGDDFFRYVNGAWYDKAVIPSDRTSTGAFVDLDVESEDRVRGIMGD
                     LETRKDRLTPAEKRVRDLYHSYVDTARVERFGLKPAEKDLQAIAAVRSLDDVARAMAS
                     VAMGTQSIFRTGIGIDDKNPDAYVVTLGQSGLGLPDRDYYLLDEKGIVAARDAYRTYV
                     AGILKLGAIADADSKATAIFDLETAIAKLHWARAERRNAEKTYNPMSVAELETFAPQF
                     PWRLYLKELGISGVEQGTRRVVVSENTAFPQLAALFANTPVSVWRDYLTYHYLSSHAS
                     YLPQRFDDARFAFYGKVLGGQMQQLAREKRGVRFVDGVIGQEVGKIYVAKYFSPEAKA
                     KAQNLVANLLDVYRQRIQAADWMSPATRQKALEKAANFTVKIGYPDKWRDYSKFQTDA
                     DDLLGNAERASLFEWNRKLVRLDQPVDRSEWEMSPQTVNAYYEESLNEIVFPAAILQP
                     PFFDPNADDAVNYGGIGAVIGHEISHGFDDQGSKYDAKGVLQNWWTPSDRKNFEARTA
                     SLAKQYGTYSPVKGMFINGELTLGENIADLAGLTVAQAAYHLSLKGKEPPVLDGFTGD
                     QRLFMGYAQVWRYKAHEETARQRLLSDPHSPPEFRISGAVRNVDAWYQAFNVKPENRL
                     FLAPEARVHLW"
     gene            complement(4260..4580)
                     /locus_tag="VGM72_01940"
     CDS             complement(4260..4580)
                     /locus_tag="VGM72_01940"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HEY4264052.1"
                     /translation="MSDPASASATPPPPPTDLPPFRPRPAFPWARLFYAIGFSFVAWA
                     VFWLIVALLAPLHYITIAITGRANEELRTMSLRAVHYLFELLAYITGARDSQPFPLGP
                     FPKD"
     gene            complement(4617..5339)
                     /locus_tag="VGM72_01945"
     CDS             complement(4617..5339)
                     /locus_tag="VGM72_01945"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY4264053.1"
                     /translation="MDSGSRDFYARGFMAFVQDDRPARAPFLNAPPIVLWLIGLLLAT
                     HTVRVLLPGNLPDAILENYAFIPARFADAMAQGVSPGGLAGLGVCFISYIFLHGDFTH
                     VGINSLWLLVFGPIVARRLGAARFVLFFLLCGVAAAAVHLAVYWGSAMAVVGASGGVS
                     GLMAAGMRILYGRMFGDAKGLAPIFSKPILAFSAVWMVANIIGGVLRLGVTDDLTLIA
                     WVAHLGGYFAGLVMIAVFDRHA"
     gene            5661..5927
                     /locus_tag="VGM72_01950"
     CDS             5661..5927
                     /locus_tag="VGM72_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264054.1"
                     /translation="MRILDEAGIDARVDMVRLEALAEAAYDAMYEARLHGARNRYEEA
                     RYYFERAMEAAHRARLYEEAARLKRRRDHVGRVYNSQFRYSGGG"
     gene            6105..6761
                     /locus_tag="VGM72_01955"
     CDS             6105..6761
                     /locus_tag="VGM72_01955"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY4264055.1"
                     /translation="MTWTIIATCIVVFFWQLSFSESQEEALIYALGFVPKNLFDNAEI
                     TRFYGIPWPWFTLMTSMFLHGGFLHLGGNMLYLWIFGNNVEDAMGHVRFLAFYFVCGI
                     VAALSEGLVNPHSGLPMLGASGAISGVLAAYVLIYPRTRITVIIPLGILLYPTKISAF
                     YVVGFWFLLQLLNVVGTTAGGPGTAWWAHVGGFVAGMLLTPMLSNFPLFGRYRRGPWG
                     "
     gene            6894..7580
                     /locus_tag="VGM72_01960"
     CDS             6894..7580
                     /locus_tag="VGM72_01960"
                     /inference="COORDINATES: protein motif:HMM:NF018960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY4264056.1"
                     /translation="MKHRNSHFAVGYWSRIRHGRATPDQVDIDPKALKRLLPFVFLLD
                     ARGGAFTYRLAGTTLCERYGSELRGRDFLYQWDADSRVRLNELLRRSLQVCVPVCLTS
                     IGATDDCHMVEIETVLMPISFGGAYPERFLGVAQVLTDVSPLAGQAISFERLVSSALI
                     REDEPAEQASPPPNDRGRPHPRAPHLRLVAGRESVRQSDKDPLRMDEETIKTFLGFFQ
                     KPRNRSVEGF"
     gene            complement(7590..7949)
                     /locus_tag="VGM72_01965"
     CDS             complement(7590..7949)
                     /locus_tag="VGM72_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264057.1"
                     /translation="MKNDVRRSVFAALALALWMGSSAAQTLPPTAGRDLSVGTGTFGV
                     RNMDFDLWCQQTQRYDAARCAARRAEDIKAFEDYRSSIERYELDYLKQVQKDQETRAR
                     TNRDPSQTVSGKQDGVP"
     gene            8164..8838
                     /locus_tag="VGM72_01970"
     CDS             8164..8838
                     /locus_tag="VGM72_01970"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY4264058.1"
                     /translation="MIARALKERRRFRRVHVAITGRLYIPATQEEAICTVEDISPGDA
                     CILCELREEPRGRAVIYLDTLGRFEGPVVRARNGGFVMTFSCSLQKREKLADQLALEM
                     NRHLMSEADLRRYDRVEATNGSFTHFTRSTGEQMRCEVMDLSLTGVSVRTEHKPPIGE
                     HILIGHRAGRVARHHGDGIGVEFLGGGAAHAESPPTAAPMRVIMAPATRMAAQPQPAL
                     AQAARR"
     gene            complement(8853..9608)
                     /gene="hisF"
                     /locus_tag="VGM72_01975"
     CDS             complement(8853..9608)
                     /gene="hisF"
                     /locus_tag="VGM72_01975"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017395071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEY4264059.1"
                     /translation="MLKIRVIPCLDVKDGRVVKGVNFVSLRDAGDPVEQALVYDAAGA
                     DELCFLDITASHEKRPILLDVVRRTASACFTPLTVGGGVRSTEDIRALLLAGADKVSI
                     NTEAVRRPEFIREAAEKFGSQCIVAAIDAKGVNGRFEVFTHGGRNATGIDAVEHAVKL
                     AAYGAGEILLTSMDRDGTKAGYDIALTRSVAGAVGIPVIASGGVGSLDDLVAGVTEGH
                     ANAVLAASIFHFGEATIRDAKARLAAAGLCVRL"
     gene            complement(9708..10739)
                     /gene="holA"
                     /locus_tag="VGM72_01980"
     CDS             complement(9708..10739)
                     /gene="holA"
                     /locus_tag="VGM72_01980"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEY4264060.1"
                     /translation="MDIKSHLADRFVSAPPSTLVAALFYGPDQGMVRERAARLAKSVV
                     PDLSDPFRVAELDDAALDSDPARLWDEAAALSMIGGRRVVRVRGAGNGLAKDFERFLS
                     DPKGDALIVVEAGELAKNAALRRIFEEADNAAAIACYPDNERDLEEVVRSALKDFGLG
                     IEPDALDDAVSRLGSDRGVTRVELEKLALYAMGEKTVSQAHVAAIMGDESELRMDETL
                     DAAGEGNYAGLDMSLSRLWAAGTSPVAVLRQALTHFQRLLLVRAQTDEGNDTKAAMRK
                     LRPPVHFAREADFRGQVSRWTAARLEEALTLLYEAEALVKTTAVPAEAACGRALLSVA
                     ALANARRAA"
     gene            complement(10750..11256)
                     /gene="lptE"
                     /locus_tag="VGM72_01985"
     CDS             complement(10750..11256)
                     /gene="lptE"
                     /locus_tag="VGM72_01985"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEY4264061.1"
                     /translation="MATAMGMLLLTGCGFHPLYGNTALDPHVGATLANVYVEPISDRV
                     GYALRNNLLDLFNATGEANGATYRLKLTLSEMEEAVVLQTNTAITRYNYTLAAHYELI
                     PRESATAVKMGDVTALSAYNVAAAPFLYATVTAERDAKNRAASDIAERIRTQIAVYLR
                     NTASQPQH"
     gene            complement(11523..14075)
                     /gene="leuS"
                     /locus_tag="VGM72_01990"
     CDS             complement(11523..14075)
                     /gene="leuS"
                     /locus_tag="VGM72_01990"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEY4264062.1"
                     /translation="MARYNAKESEARWQEAWSTRGVFAAVNQSALPKCYVLEMFPYPS
                     GRIHMGHARNYTMGDVIARFKRAQGFNVLHPMGWDAFGLPAENAAREKGVHPREWTYA
                     NIAAMREQLKAMGLAIDWSREFATCDPEYYARQQKLFLDFFRMGLVYRAEADVNWDPV
                     DSTVLANEQVIEGRGWRSGAIVERRKLSQWFFRITQFADDLLTGLDSLERWPEKVRLM
                     QKNWIGRSEGLRFSFALKDAPEPGVEVFSTRPDTLFGASFVALSPDHPLTEQLAARDA
                     KLAAFVAECHRGGTAEETIETAEKMGYNTGLRATHPLDPNWQLPVYVANFVLMAYGTG
                     AIFGCPAHDQRDLDFARKYRLPVLPVVIPDDADTKSFTIGTEAYVGSGHLANSRFLDG
                     LDVDAAKNEVAKRLEAAGTGARTVHYRLRDWLVSRQRYWGCPIPIVHCGMCGMVPVPE
                     RNLPLKLPDEVRFDLPGNPLENHPAWKHVKCPVCGGAATRETDTFDTFVDSSWYFARF
                     TDSHADAPVNPDAARYWMPVDQYIGGVEHAVLHLLYARFFTRAMAKAGDIAVQEPFAG
                     LFTQGMVCHETYRDADGEWLLPEDVEKRDGQAFRRGSDAPVAVGPSEKMSKSKKNVVA
                     PEGIIDAFGADTIRWFMLSDTPPERDVEWTQEGAEGCWRFVQRIHRLVTEAGALPPPG
                     AKPAQDCGGADLELRRATHRAVAAVTEDLAALRFNRAVAQIYTLANALSASGPVDGAV
                     KREALEVLVLLIGPMMPHLAETCWEALGHKTLVATAHWPVANKGLVTSDTLVIAVQVN
                     GKRRGEIEIARAAQEEEVKASALALDSVQRALEGKTPRRVIVVPGRIVNVVA"
     gene            complement(14168..14659)
                     /locus_tag="VGM72_01995"
     CDS             complement(14168..14659)
                     /locus_tag="VGM72_01995"
                     /inference="COORDINATES: protein motif:HMM:NF023522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3576 domain-containing protein"
                     /protein_id="HEY4264063.1"
                     /translation="MRILILAATACLLGACSSDSSTQSAAPSTASTSTNNGSWFWPFG
                     GSSDDQTASDMPQLGVNSYLWRATLDTLNFMPLSSADPVGGVVISDWYAAPDKPDEHL
                     KVTVYILDKRLRADAVKVSVFRQVRNPNGWADATVNPDTGIKLENAILARARELRLAT
                     TPQ"
     gene            complement(14813..15517)
                     /locus_tag="VGM72_02000"
     CDS             complement(14813..15517)
                     /locus_tag="VGM72_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264064.1"
                     /translation="MRIISLVACVALSGAAGFAGTGSALAADCNKGMLWPFVRTAGDC
                     LTDVEIASGKTGVYNGPVNTNVDLSTVKPSEPPVQNNNDSNSGGLLGGSLFGGSLFGS
                     GSGSSGNNSGDGALITSTSVFGGGPASRTATTVNCNKGTLWPFSREPGDCLTEGEKKA
                     GKTGVYGGGPGVMPVGATATATATAAADSAQTSSASPSPPAAAPPATCQRGWFWPFVR
                     ESNDCPTEADKKSSGK"
     gene            15668..16900
                     /locus_tag="VGM72_02005"
     CDS             15668..16900
                     /locus_tag="VGM72_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY4264065.1"
                     /translation="MIQKKKRVAPLLAAAALFGGFAPACMADAAEDSAGIAQELALEP
                     PQFDLGDINVKLGGFAGAAFFNAHQSAGPAYGSYDFARTSGEARANIRAQRIFDNGLI
                     LGAGGDFLLYHDSLSGDQYGNDFVEKAFLFAQTGFGRVEIGEADGAGYTLGLTGPVTN
                     PEVTLENRNISLFHDPVAGEDFARIFEAVAAVQSTSNFAKINYLTPRLFGIQIGASFT
                     PETLRAPLPFTGNPSNLSDRQHDIWEIAASYTGYVSNVALGLSAAYAHGSLKNESAGA
                     DADGLYDWSLGAQAATMLSDVRLSFGGGYRESNAYLLETDTVLAHGGTHTGHISATAE
                     WKRWIAGVEYSLSEISGPVDFDIRGYQVSAGYRLNDNLQLTAGWQWYDYRRNFGTFYN
                     GGPGIDMNAGFLALSYEL"
     gene            complement(16907..17527)
                     /locus_tag="VGM72_02010"
     CDS             complement(16907..17527)
                     /locus_tag="VGM72_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEY4264066.1"
                     /translation="MASVLARVKLARAARLLNAAAPHLPPLILMTDQTRLADPLAAAR
                     ALPKGAAIILRHTEAAPRAALARALHKIARQRGLRLLIAGDAGLAARLPCDGLHLPEA
                     RAGEAVHWKALHPDWLITASAHSRRGLGVAARARCDAVLLAPVFATQSHIGREPFGVS
                     RFCTLAGSARLPVYALGGVNATNVQRLAGTRLAGIAAIEALIPAHS"
     gene            17531..18223
                     /locus_tag="VGM72_02015"
     CDS             17531..18223
                     /locus_tag="VGM72_02015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEY4264067.1"
                     /translation="MTSENPPISVDPAGNLKAVLARIAAARRCAIAPADETHLIAVSK
                     THDEARILPVLEAGHRIFGENRVQEAKSKWPQLRARFADVTLHLMGPLQTNKVRDAVA
                     IFDAIHSLDRLKLAEALRDECARTGKRIALFVEVNIGAETQKAGLAPHETARFVRHCR
                     DGLNLNVVGLMCIPPAKEEPALFFALLRKLARECGLNALSMGMSDDFETAIRFGATHV
                     RVGTAIFGERET"
     gene            complement(18236..19039)
                     /locus_tag="VGM72_02020"
     CDS             complement(18236..19039)
                     /locus_tag="VGM72_02020"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEY4264068.1"
                     /translation="MFRRGQFMIAVRKKFHDEANAKPARRIKRELLKIDGKSIEVILR
                     TNPRARRFIVKVDPATGEVSVVSPSSRSFDRALDFARKEKDWIAGRLADVPDAVQIDI
                     GSTILFKGVEYAIRPGEGKRAPVWIDREAARPTIRVGGRPEHASRRLLDWLKREARMK
                     IDERVAEHAARLGVRPKRITIRDTSSRWGSCSSARSLSFSWRLVLAPPAVLDYVVAHE
                     VAHLRELNHRPRFWRLVELLVPDIEKSQAWLSEHGAMLHRYAPRMTIIE"
     gene            19339..19854
                     /locus_tag="VGM72_02025"
     CDS             19339..19854
                     /locus_tag="VGM72_02025"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEY4264069.1"
                     /translation="MALAIGKDHALSGSAGTDAALFVGAVSAALLLGAFGSQYLGGLA
                     PCEMCIWQRWPHGAAILLGLIGGGLVRGGVLPPNLGRTLAILAILAIAISGAIGVFHA
                     GVEWKLWPGPAECTGVGYVPGRDDFKPLQIVRCDEAQWRLFGISLAGYNAVFSLAAAA
                     LAASWLRRARA"
     gene            19851..20462
                     /locus_tag="VGM72_02030"
     CDS             19851..20462
                     /locus_tag="VGM72_02030"
                     /inference="COORDINATES: protein motif:HMM:NF015207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HEY4264070.1"
                     /translation="MNSRTMSPSARTANRGARPVLPGTQDACAADIEAMIRVDHAGEF
                     GALRIYEGQLAVLSRKTPASEAQSDVQSIRHMAAQEQTHFDTFERMIEERSVRPTALE
                     PVWRIAGFALGAATALMGEKAAMACTVAVEDVIDEHYSRQIERLGENEPDLKQTIQKF
                     HAEECAHRDEALERGAREAPAYPLLSAAIRLSCRMAIALSERL"
     gene            20459..21103
                     /locus_tag="VGM72_02035"
     CDS             20459..21103
                     /locus_tag="VGM72_02035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEY4264071.1"
                     /translation="MNASAQAPIRRATEADVPRLARLFAAAFARDPLFDWLARAGSKR
                     QRALQRFFGWVLARRTVPHGETWISADGLAAAAWIPPYVKSAPPRFLEDLRTLPAILR
                     LTGLKRLPRGAAMAAAMDEAPPQTPYFYLAFIGVAPRMQGAGLGSALLRQTLARADAA
                     GSGAYLENSNPRNLKLYQRAGFAVLREVKAHADAPPVTAMWRPAKARQSGIPSD"
     gene            complement(21255..21815)
                     /locus_tag="VGM72_02040"
     CDS             complement(21255..21815)
                     /locus_tag="VGM72_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY4264072.1"
                     /translation="MLAAERVPDNCPALVLNADYRPLSYFPLSLWSWQDTIKAVFLER
                     VNIVAEYERTVRSASFQMRLPSVVSLKTYVRPTRNPAFTRFNVFLRDRFQCQYCPSKE
                     DLTFDHVIPRSRGGRTTWDNVVAACAACNLMKGGRLPHRAGMHPRNSPHKPSVAELQN
                     NGRMFPPNYLHQSWADYLYWDIVLEP"
BASE COUNT         4258 a         6857 c         6622 g         4183 t
ORIGIN      
        1 caaccagtct cggcatcggc atttcgctcg cgcagacgat cttcgacggc gggcggctga
       61 cgggcgaatc cgatgaagcg cgtgcccgcg agcaagagct gatcgcaaat tatcgcaata
      121 ccgccatcgt ggcgttttca gaggtcgaaa cctctctcgg cggaatcgcc cattttcgcg
      181 agctggaagt ctatcaggcc gaacaggtgg cgcagtcgca aatcgcgttc gacatcgcac
      241 aggtgcgcta ccgcgaaggc gtcgccgatt atctgacggt gctcgatgcg cagcgcacac
      301 tctattcagc gcgcgattcg cttggacaga tcaaactgca gcggctgcag gccatcgttg
      361 cgctctacaa agcattgggc gggggctgga ccgagccatc ctcctcaacg ctcgcacgcg
      421 attaggcgtt acttgccccc ggcgggtttt gccggagccg gcgcagcacg gccggccacc
      481 gggccgggca aggtgggcgg cggcgccacc ggcaggggat tggcaccggc gggcagtgcg
      541 accgcgcgat ctttcgcata gcccttcatc cggtctaagg cctcaggaaa gcccgtcagc
      601 gacaccggca atttgatctc gttcgcctgc ccgtagccaa cgacaccgat ggtggttgat
      661 tttccagccc cggaaatcga ggcgaccgcc gcattgtcga gcagcatttc gacatagcac
      721 ccgtcgcgct cacagcggtt gaacttggcg ccattcagcg cagtgccgag ctgcaaaccc
      781 ttggacagcg caactccggt cggcacgacg acctgcaccg cgtaagcatc ccggcttggc
      841 acataagcaa gcgagaaggc cgaaatgcgt tgcttgttgt ccttgttcac cgtcgtctgc
      901 atgacttcgc acggcgccgg gctcttcacc gttgtctgta cacagcgcac cacccaatcg
      961 ccgacactct gattgatgga atattggggg ggcgccggcg gagcggcggg cggagcctcc
     1021 ggtggcggag ccgccgcagc ttgctgcgcg gcgattgccc ctcctggaag gatagccagt
     1081 aacgcccctg ccaacgcgaa gcctgcaaga ttacggaaca tgtcggccat cctcacgatc
     1141 atcagcgatt taagagcgat tgagaaagct cagcataccg gtgcgccgcg ccttggcccg
     1201 ctggagttcg ccaaagccat cggcaaatcc tttaagcgaa tatggaatat taatgggctg
     1261 cgggttgttg ggggccgcta caaccacgtg cccacccata ttggcatcga gcgccttcag
     1321 agtgtcggca tccatggtga ccagggcaat gcaaccctga ttgctgcagg tctcgtaagg
     1381 ccgcacccgt gtcggctcag tgccgatcgc aaagcccagg cccggatcaa gcagaacgcc
     1441 gtggggaaca gtgagcgaaa gttgactgcc aggcgcgggg tcaagcaccg agagcgtcaa
     1501 caaggttcct ccggtatccc gcagtacatc ggaggtaagc gcgcagttgg gcgtcgtttt
     1561 tgtcagtggc ggacaaatca cacgccaatc ctgaaacgta ctgatgcgca tctcattcgg
     1621 tgcaattccg ccgagcagac gttcggcggc taaggcgatc acgccgccag ccgcgagcag
     1681 gagtatgaca atccccccgc gcaacgccca acgtccccag cgcccgccgt ccatggatcg
     1741 cgtgcctgct ctcacgcgtt ggttgctggt ttcagtcatc gaaattccac ccgatcagat
     1801 atcgctgttc cccgcgggaa gcggcaagcc gcgctccatg gcgaatatgc ccggcttttg
     1861 tacaaacccc acccgtccgg ctcgattggc cgccgcgtgg cacacggcgc tcaaccgata
     1921 aaacgcccaa ccgttaacgc cctcctgcag gcaaggcaag cgcaatgctg ttaaggtgct
     1981 taagacaaac ggacttctcg cattaccaaa gatgcacacg ggcctctggc gcgaggaaga
     2041 gcctgttctc cggcttcaca ttaaaagcct gataccaggc atccacatta cgcaccgcac
     2101 cgctgatacg aaattcaggc ggactatgcg ggtctgatag gagcctctgc cgcgcggttt
     2161 cctcatgggc tttatagcgc caaacctggg catagcccat gaacagccgc tgatccccgg
     2221 taaatccgtc gagaacgggc ggctctttgc ccttaagcga gagatgatag gccgcttgcg
     2281 caaccgtcaa tcccgccaga tctgcgatgt tttccccaag cgtaagttcg ccgtttatga
     2341 acatcccttt gaccgggctg taggtgccgt attgcttcgc cagagaggcc gtgcgcgctt
     2401 cgaagttctt gcggtccgat ggtgtccacc aattctgcag gacgcccttg gcgtcgtatt
     2461 tgctgccctg atcgtcgaaa ccatggctga tctcatggcc gatcaccgcg ccgatcccgc
     2521 cataattgac ggcatcatcc gcgttgggat cgaaaaaagg cggctgcaga atcgccgcgg
     2581 gaaaaacaat ctcgttcagc gactcttcgt aataggcgtt gaccgtctgg ggcgacattt
     2641 cccattcgct gcgatccacc ggctgatcga gccgcacgag cttacgattc cattcgaaca
     2701 aggacgcgcg ttccgcatta ccgagaagat cgtccgcatc tgtctgaaat ttggaatagt
     2761 cgcgccattt gtcggggtag ccgattttaa cggtgaagtt ggcggctttc tccagcgcct
     2821 tctgacgtgt cgccggcgac atccaatccg ccgcctggat gcgctgacga tagacatcta
     2881 gaagattggc cacgagattc tgcgctttgg ccttggcttc cggtgaaaaa tatttggcga
     2941 cgtagatctt gccgacttct tggccgatca cgccatcgac aaagcgcaca cctcgttttt
     3001 cgcgcgccaa ttgctgcatc tggccaccca gaaccttgcc gtagaaggca aagcgcgcat
     3061 cgtcaaaacg ctgcggcaga taggaagcgt gactggaaag atagtgatat gtcagataat
     3121 cgcgccacac cgaaacgggc gtattcgcga acagcgccgc aagttgcgga aaagcggtgt
     3181 tttcggaaac gacaacccgt cgtgttccct gttcgacccc ggaaatgcca agttctttga
     3241 gatatagcct ccaagggaat tgcggcgcga atgtttccaa ttccgccacg ctcatcggat
     3301 tgtaagtctt ttcggcattg cgccgctcgg cgcgagccca atggagcttt gcgattgccg
     3361 tctcaagatc gaatatggct gtcgccttgc tgtctgcgtc agcaatcgcg cccagcttca
     3421 ggatgcccgc aacataggtg cggtaggcgt cgcgtgctgc gacaatgccc ttctcgtcca
     3481 acagataata atcgcggtcg ggcaagccca atcccgattg accgagcgta accacatagg
     3541 catcggggtt cttgtcgtcg atgccgatgc cggtacgaaa gatgctttgc gtgcccatcg
     3601 cgaccgaggc catcgcgcgt gcgacatcgt ccagagatct cacagcggcg atcgcctgca
     3661 gatctttttc cgccggcttc agcccgaacc gctcgacgcg ggccgtatcg acgtagctgt
     3721 gatacaggtc tcggacgcgt ttctcggcag gagtcagccg gtccttgcgg gtttcgagat
     3781 cccccatgat cccgcggaca cggtcctcac tctcaacgtc gagatcgaca aacgcaccgg
     3841 tgctggtgcg atcggacggg atgacggcct tgtcgtacca ggcgccgttg acgtagcgga
     3901 agaaatcgtc tcccggtgcc acggtcttgt ccattccgga aagatcgacg ccccaattcc
     3961 cgaacgtagg cttcttggcg atagcggtcg cctgattgga gaccggcgcg gtctgggcgg
     4021 caatcgcgcc catcgcggat atggtcagca aaatgcccag aacagccaac cctgtccttt
     4081 tcatgcgata gtccttcttt gcgcttttgg tattcgactc aatgttcaaa ctgacttgga
     4141 gcatttgtga tgcttggata cgagggctgt ggtttcgcct ccgtccaatc aatagtgcgt
     4201 taatgccttt aattcgcgct agtctacttc attcgatttc ggagggcgag gttgaaacct
     4261 cagtccttgg gaaaaggtcc gagcggaaac ggttggctgt cacgcgcgcc cgtgatgtag
     4321 gccagcagtt cgaaaagata atgcaccgcc ctcaagctca ttgtgcgtag ttcttcattt
     4381 gcccggccgg taatcgcgat ggtgatgtaa tgcagcggcg ccagcagtgc gacgataagc
     4441 cagaaaaccg cccaagcgac gaagctgaac ccaatagcat agaaaagcct ggcccaagga
     4501 aaagccggac gaggacggaa cggcgggaga tcggttggag ggggtggtgg tgtcgcagac
     4561 gcggaagcgg gatcgctcat ctcaactcct tccatccggc gcggaattct ctcgccctag
     4621 gcatgccggt cgaacaccgc gatcatgacg agccctgcaa aatagccccc gagatgcgcg
     4681 acccatgcaa tgagcgtcag atcgtccgtg acgccgagcc gaagcacgcc gccgatgata
     4741 tttgcgacca tccagactgc ggaaaacgcc aagataggct tagaaaagat gggcgccagt
     4801 cctttggcat cgccaaacat gcggccatag agaatccgca tccctgccgc catcagtccc
     4861 gaaacaccac cgcttgcacc caccacagcc atggccgagc cccaataaac ggccagatga
     4921 acggccgctg ccgcaacacc gcataggagg aagaatagaa cgaaccttgc cgctcccagc
     4981 ctgcgagcga cgatcggccc gaacaccagc aaccacagac tgttgatacc gacatgggta
     5041 aagtcaccat gcagaaaaat atagctgatg aagcaaaccc ccaggccggc aagcccgccc
     5101 ggagacacgc cttgagccat ggcgtcagca aatcgcgcgg gaataaaggc ataattctca
     5161 agaatagcgt ccggcagatt tccgggtaaa agcacgcgga cggtatgcgt tgcaagcaat
     5221 agtccgatca gccagagcac aatcggtggt gcattcaaaa atggcgcacg tgcgggacgg
     5281 tcgtcctgaa caaaggccat gaagcccctc gcgtaaaaat cccggctgcc gctgtccatc
     5341 cacgctcgag gcactgccag cctctttcat attacctcgc acccagcggt acggcgagac
     5401 acggcgtgtg gttatctttg cgaccaaact caacctgcag aagcaatttc ctgaacaaaa
     5461 gagcgatgaa cttttcctct cgccgcatct gtattaaacc tgtgcagtgc gtttgacctt
     5521 gcagcagaca tgttggtttg atttgatact cgttctacac ttgcctgagt gaggctgggg
     5581 agtggtagcg gatgaccatc agtgactggt ggcacaagcg tcgcgaacgc cgggcacgca
     5641 gagaaaaagc gaggcctctc atgcggattc tcgatgaggc cggcatcgac gcgcgggtcg
     5701 acatggtgcg gctggaagcg cttgcggaag ccgcttacga cgccatgtac gaagcgcgtt
     5761 tgcatggcgc caggaaccgc tatgaagagg cgcgctatta tttcgaacgc gccatggaag
     5821 cagcccatcg tgcacgtctt tacgaggaag cagcccgtct gaagcggcgg cgtgaccatg
     5881 tgggacgggt ttataacagc caattccgct attccggcgg cggctgagag cgctcctctc
     5941 agctattttc gatgagcgcg ggaacccggt taccgctccg gaaaaggggg tgagcggcga
     6001 acgtgtgtca tatcgaaaca gcaaggccgt tatgacgggc gaggcggccc aaatattcca
     6061 tgatccccat ctcggacgat aacccggcac gattaagacc gatcgtgacc tggaccatta
     6121 ttgcaacctg cattgttgtc tttttttggc aactttcctt cagcgaaagc caggaagaag
     6181 cccttattta tgcgcttggc tttgtgccga agaatctctt cgataacgct gaaatcacac
     6241 ggttttatgg gattccctgg ccctggttca ccctgatgac ctccatgttt ctccatggag
     6301 gcttcctgca cctcggcggc aacatgcttt acctctggat cttcgggaac aatgtcgagg
     6361 acgcgatggg gcatgtgcgt tttcttgcct tctatttcgt ctgcggcatc gtggcggcgc
     6421 tctcggaggg cctcgtcaat ccacactctg ggttgccgat gctgggtgcc tcgggagcga
     6481 tttccggggt tctggcagcc tatgtgctga tttatccacg cacacgcatc acggtcatca
     6541 tccccctggg aatccttctc tacccgacca agatttcggc gttttacgtc gtcggcttct
     6601 ggttcctgct gcaactcctg aacgtggtag gcactacggc gggcgggcct ggaactgcgt
     6661 ggtgggcgca tgtgggcggc tttgtagcgg gcatgcttct cacccccatg ctttccaatt
     6721 tccccctttt cggacgctac cggcgcggcc cctggggata gtctgcgcat tcgggaacgt
     6781 accgcttcgg gcggttggca cggctttcgc tgtttgcttc ccgcgcgacg tgccgtcttc
     6841 ccattttggg gcagacgtga aacggcgcgc gaattccggt ggagcgtttt ctcatgaagc
     6901 atagaaacag ccattttgcg gttggatatt ggagcaggat ccgtcacggc cgcgccactc
     6961 cggatcaggt cgacatcgat ccaaaggctt tgaagcgtct gctgccgttc gtattcttgc
     7021 ttgacgcgcg tggcggcgca ttcacttacc gtttggccgg gacgacgctt tgcgaacgct
     7081 atggcagcga gttgcgaggg cgggattttc tttaccaatg ggatgccgac agccgggtgc
     7141 ggctgaacga gttgctgcgc cgttcgctgc aagtctgcgt gccggtctgc ctgacctcga
     7201 tcggtgcgac cgacgattgc cacatggtcg agattgaaac cgtgctgatg ccgatttcgt
     7261 tcggcggagc ctatcccgag cgtttccttg gggtcgctca ggttttgacg gatgtctcgc
     7321 cgctcgccgg tcaggcgatc agcttcgaac gcctggtgtc ctcagccttg attcgtgaag
     7381 acgaaccggc ggaacaggcg tcgcccccgc ccaacgaccg tggccggcct catccccgtg
     7441 cgccgcatct gcggctcgtc gcagggcgcg aaagcgttcg tcaaagcgac aaggacccgc
     7501 tgcgcatgga tgaggagacc atcaagacat tcctcgggtt ctttcaaaaa cccaggaacc
     7561 gttcggtcga aggcttctga tcccaaggat tacggcacgc cgtcctgctt tccgctgacc
     7621 gtctggcttg ggtcgcggtt ggtgcgcgcg cgcgtttcct gatccttttg cacctgcttg
     7681 agatagtcca actcatagcg ttcgatggaa ctgcggtaat cctcgaatgc cttgatgtcc
     7741 tccgcgcgcc gtgcggcgca gcgcgctgcg tcataacgct gtgtctgctg gcaccaaaga
     7801 tcgaaatcca tgttgcgtac gccaaatgtg ccggttccaa cagacaggtc gcgtccggcc
     7861 gtaggaggaa gggtctgggc cgcgctgctt cccatccaaa gggcgagagc aagtgcggca
     7921 aaaaccgatc tgcggacgtc atttttcatg agacagcctc cccattagaa accatatcct
     7981 ccctgaaagg ttccaggaag cgccaaccat cgaagcggcc ccggcaaagt tacgcgaaag
     8041 ccctctccgt aaacgcttta ttgagcagtc agacgataat ttggcggccg gaatcggaaa
     8101 aaccgggtca ccgcgcgtat gaacgctcct gaagagaaca cagacaagga cgctggccaa
     8161 aaggtgatag cgcgcgcgct caaggagcgc cgccgcttcc gccgcgtcca tgttgccatc
     8221 accggccggc tctacattcc ggcgacgcag gaagaggcga tctgtacggt tgaggacatc
     8281 tcgcccggcg atgcatgcat cctctgcgaa ttgcgcgaag aaccgcgcgg gcgcgccgtc
     8341 atctatcttg acacgcttgg acgtttcgag gggccggtcg tgcgtgcgcg caatggcggc
     8401 ttcgtcatga cgttttcctg ctcgcttcag aagcgcgaaa agcttgccga tcaattggcg
     8461 ctcgaaatga atcgccacct catgagcgaa gcggatctgc gccgctatga ccgggtcgag
     8521 gccaccaatg gcagcttcac gcatttcacc cgctccaccg gcgagcagat gcgctgcgag
     8581 gtcatggacc tgtcgctcac cggcgtctcg gtgcgcacgg aacacaaacc gcccataggc
     8641 gagcacatcc tgatcggcca tcgcgcggga cgtgtcgcgc gccatcatgg cgacggcatc
     8701 ggagtcgagt ttttgggcgg tggcgccgcc catgcggaaa gccctcccac cgcagcaccg
     8761 atgcgcgtca tcatggcgcc ggcgacgcgt atggctgccc agccccagcc tgcattggcc
     8821 caagccgcgc gacgctagag cctcacgtag agctaaagcc tgacgcacag gccagccgcg
     8881 gccagacgcg ctttagcgtc ccgaatggtc gcttcgccaa aatggaagat cgaggctgcc
     8941 agaaccgcat tggcatggcc ctcggtgaca cccgcgacga gatcgtcgag cgaacccact
     9001 ccgcccgagg cgatgaccgg aatgccaacc gcgccggcca cgctgcgtgt cagcgcgatg
     9061 tcatatcccg ctttggtgcc gtcgcggtcc atcgaagtga gcaaaatctc gcccgcgcca
     9121 tatgcggcga gcttcaccgc gtgttcgacc gcatcgatgc ccgtcgcgtt gcgtccgcca
     9181 tgggtgaaga cctcaaagcg tccattgaca cccttggcgt cgatggcggc gacgatgcat
     9241 tggctgccga atttttccgc ggcctcgcgg atgaattctg gccggcgcac ggcctcggta
     9301 ttgatcgaga ccttgtccgc acccgcaagc aatagcgcgc ggatatcttc cgtgctgcgc
     9361 acgccgcctc cgaccgtcag tggcgtgaag cacgcggacg ccgtgcggcg cacgacatcg
     9421 agcagaatgg gacgcttctc atggctggcc gtgatgtcca ggaagcagag ttcgtcagcg
     9481 ccggcagcat cgtaaaccaa cgcctgctcg accggatccc cggcatcgcg caagcttacg
     9541 aagttcacgc ccttgacgac ccggccatcc ttcacgtcga ggcacgggat gacgcggatt
     9601 ttcaacatgg gacggccatt ctttgcttgt tcgttctcgc ggcaaagccg ctgcggacga
     9661 acgggccgtt cacgtcaagg catgggatga cgcgaatttt cagcatctca cgccgccctg
     9721 cgcgcgttgg cgagcgctgc gaccgaaagc agagcccggc cgcaggccgc ttccgccggc
     9781 accgccgtcg tcttcaccag cgcttcggct tcgtagagaa gcgtgagggc ctcttcaagc
     9841 ctagccgccg tccagcgcga cacctgtccg cgaaagtctg cctcgcgtgc gaaatgtacc
     9901 ggcgggcgga gttttctcat cgccgctttc gtgtcgttgc cttcatcggt ttgcgcgcgc
     9961 accagaagca gccgctgaaa atgcgtcagc gcctgacgca ataccgcgac gggtgacgtg
    10021 cccgccgccc aaagccgcga cagcgacatg tccagccccg cataattgcc ctcgcccgcg
    10081 gcgtccaacg tctcgtccat gcgcaattcg gattcatcgc ccatgatcgc cgcgacatgg
    10141 gcttggctca ccgtcttttc gcccatcgca tagagcgcga gcttctccaa ttcgacgcgg
    10201 gtgacgcccc tatccgagcc caaccgcgaa accgcgtcat cgagcgcatc gggctcgatt
    10261 cccagaccga agtccttcag cgcgctgcgt accacttctt ccagatcgcg ctcattgtcg
    10321 ggatagcagg caatcgcagc tgcgttgtcc gcctcttcga agatgcgccg cagcgccgcg
    10381 ttcttcgcca gctcgccggc ttccaccacg atcaaggcat cgcctttcgg atcggaaagg
    10441 aagcgctcga aatccttggc cagcccattg cccgcgccgc gcacgcgcac cacgcgccgt
    10501 ccgccgatca tggaaagcgc cgccgcctcg tcccagagac gcgccggatc ggaatcgagc
    10561 gccgcgtcat cgagttcggc gacacggaag ggatcgctca ggtccggcac cacgcttttc
    10621 gcgagccgtg ccgcgcgttc gcgcaccatg ccctggtccg gaccatagaa cagcgccgcc
    10681 accaatgtgc tcggaggcgc gcttacgaaa cgatccgcca aatgggattt gatgtccatg
    10741 ccgcgtccgt cagtgctgcg gctgactggc cgtattgcgc aagtaaaccg cgatctgggt
    10801 gcggatgcgt tcggcgatgt cgctggcggc gcggttcttg gcatcgcgct ccgccgtcac
    10861 cgtggcatag aggaacggcg ccgctgcaac attgtaggcc gagagcgccg tcacatcgcc
    10921 catcttcacc gccgtcgcgc tttcgcgcgg aatcaattcg taatgggcgg cgagtgtata
    10981 attgtagcgg gtgatcgcgg tgttggtctg aagcacgaca gcttcttcca tttcggaaag
    11041 cgtgagcttc aggcggtacg tcgcgccgtt tgcctcaccc gtcgcgttga agagatcgag
    11101 cagattgttg cgcaacgcat aaccgacgcg atcggaaatg ggctcgacat agacgttcgc
    11161 aagggtggcg cccacatgcg gatcgagcgc ggtgtttccg taaagtggat gaaagccgca
    11221 gcccgtgagc aggagcatgc ccatggcggt ggccatgcag cggcaccacc ggagcaagcg
    11281 attcggagca gcccccccct cccccctatt tttggccggc aggatggttt tttccgcgga
    11341 acgcctcgaa aaatctgaag tgccggtgag ggtttttggt gcgaatagcg gtgcaattgg
    11401 gtgcaataga ggcatcctac cccgccgcgc ggtcgaactc cggttccgat tcatgcgcct
    11461 aatatgggcc tgcttaagcc cgcgcgcgag ggggagccca tgccggccga aaggcgtttt
    11521 catcaggcca ccacgttcac gatgcgcccc ggcacgacga tgacccggcg cggggtcttg
    11581 ccttcgagcg cgcgctgtac cgaatcgagc gccagcgcgc tggctttcac ctcttcctcc
    11641 tgcgccgcgc gcgcgatctc gatttcgcct ctgcgcttgc cattgacctg aaccgcgatg
    11701 acgagcgtgt cgctcgtgac caggccctta ttggcgaccg gccaatgcgc cgtggcgacg
    11761 agcgtcttat ggccgagcgc ctcccagcag gtctcggcca gatgcggcat catcgggccg
    11821 atcaacagca ccagcacttc caaagcttcg cgcttgactg cgccatccac aggacctgac
    11881 gcggagagcg cgttggccag cgtgtaaatc tgggcgacgg cgcgattgaa gcgcagcgcg
    11941 gccaaatctt ccgtcaccgc ggcgaccgcg cgatgggtgg cgcggcgcaa ttcgagatcg
    12001 gcgccgccgc aatcctgcgc cggtttggcg cccggcggag gcagcgcgcc cgcttccgtc
    12061 accagccggt ggatgcgctg cacgaaacgc cagcagcctt ccgcgccttc ctgcgtccat
    12121 tcgacatcgc gttcgggggg cgtgtccgac agcatgaacc agcggatggt gtcggcgccg
    12181 aacgcatcga tgatgccttc cggcgcgacg acgttcttct tcgacttcga cattttctcc
    12241 gacggcccaa ccgccacagg tgcatcgctg ccccggcgga acgcctggcc gtcgcgtttt
    12301 tcgacgtcct cgggcaagag ccactcgccg tccgcgtcgc gataggtttc gtggcacacc
    12361 atgccttgcg taaagagtcc ggcgaagggt tcctgcaccg cgatatcgcc ggccttcgcc
    12421 atcgcgcggg tgaagaagcg cgcatacaga agatgcagca cggcatgttc gacgccgccg
    12481 atatattggt cgaccggcat ccaataacgc gcggcgtccg gattcaccgg cgcatcggcg
    12541 tggctatcgg tgaagcgcgc gaaataccag gacgaatcga caaacgtgtc gaaggtatcg
    12601 gtctcgcgcg tcgccgcccc gccgcagacc gggcatttga catgcttcca ggccggatga
    12661 ttctccagcg gattgcccgg aagatcgaac cgcacctcgt cgggcagttt cagcggcaga
    12721 ttgcgctcgg ggaccggcac catcccgcac atgccgcaat ggacaatcgg aatgggacag
    12781 ccccaataac gctgccgcga gaccagccaa tcgcgcaggc gataatgcac ggtgcgcgca
    12841 cccgtcccag cggcttccag gcgcttggcc acttcgtttt tcgcagcgtc cacatccagc
    12901 ccgtcgagga agcgcgaatt ggcgagatga ccggaaccga cataggcttc cgtgccgata
    12961 gtgaaactct ttgtgtcggc gtcgtccgga atcacgaccg gcagaacggg cagacggtat
    13021 ttgcgcgcga aatcgagatc gcgctggtcg tgcgcggggc aaccgaagat cgcgcccgtg
    13081 ccataggcca tcagcacgaa attcgcgaca tagaccggga gctgccaatt cggatccaag
    13141 ggatgcgtgg cgcgcagacc ggtattgtac cccatctttt ccgcggtctc gatcgtctcc
    13201 tccgccgtgc cgcctcggtg acattcggcg acgaacgccg caagcttggc gtccctggcg
    13261 gccaattgct ccgtcaacgg atgatcgggc gacaacgcca cgaagctcgc gccgaacaac
    13321 gtatcgggac gcgtcgagaa cacttcgacg cctggctccg gcgcatcctt cagcgcgaac
    13381 gaaaagcgca agccttccga gcggccgatc cagttcttct gcataagccg gactttttcc
    13441 ggccagcgct ccaaggaatc gagcccggtc aaaagatcgt cggcgaattg cgtgatgcgg
    13501 aaaaaccatt gcgacagctt gcgccgttcc acgatcgcgc ccgaacgcca gccgcgtcct
    13561 tcgatcacct gctcattggc gagcacggtc gaatcgacag ggtcccaatt gacgtcggct
    13621 tcggcgcgat agacgaggcc catgcggaag aaatcgagga acagcttctg ctggcgggca
    13681 taatattcgg gatcgcaggt ggcgaattcg cgtgaccaat cgatggcgag gcccatggct
    13741 ttgagctgtt cgcgcatcgc ggcgatattg gcgtaggtcc attcgcgcgg atgaacgcct
    13801 ttttcgcggg cggcattttc cgccggaagt ccgaaggcgt cccagcccat cggatgcagc
    13861 acgttgaagc cttgcgcgcg cttgaagcgg gcaatgacgt cgcccatcgt gtagttgcgc
    13921 gcatggccca tatggatgcg ccccgacgga taggggaaca tctccagcac gtagcatttg
    13981 ggaagcgcgg attgattcac cgccgcgaat acgccgcgcg tgctccacgc ctcctgccag
    14041 cgcgcttccg attcctttgc gttgtaacgc gccacgcgaa gcccccgaat ccggacctga
    14101 aacctaaccg cccccgttca cccgcgccaa ggcggtcgag ggcatcacac tgggccttgg
    14161 ctagcgttta ttgaggcgtg gtcgcaagcc gtagctcgcg ggcgcgcgca aggatcgcgt
    14221 tttccagctt gatgcccgtg tcaggattga cggtcgcatc ggcccagcca ttgggattgc
    14281 gcacctgccg gaaaaccgaa accttcacag catcggccct aaggcgcttg tccaggatat
    14341 agacggtgac cttcaggtgc tcgtcgggct tgtcgggtgc ggcgtaccag tcgctgatca
    14401 cgaccccgcc aaccggatcg gccgaagaca gcggcatgaa attcagcgtg tccagcgtgg
    14461 cccgccagag ataggaattg acgccaagct gcggcatgtc ggacgcggtt tgatcgtcgc
    14521 tgctgccacc gaagggccag aaccacgagc cgttgttcgt gctggtcgag gccgtgctcg
    14581 gggcggcgct ctgggtggaa ctgtcggagc tgcaggcgcc aagcaaacag gcagtggcgg
    14641 cgaggatgag aatccgcatg aatcgtcctt atgaactccg gtaccggacc ggcaccgaag
    14701 acaaaccccc agagccccat cggcttgggc ttataatcag gcaggacgga cggcgcaacg
    14761 gctcacccat cgcgcatgtc gcgcccggct tcggtctccc gccaaatcct cgctactttc
    14821 cgctgctctt cttgtcggct tcggtcgggc aatcattcga ttcccgtacg aacggccaaa
    14881 accagcccct ctgacatgtc gcggggggag ccgccgccgg aggagaggga gaggccgaag
    14941 aggtctgcgc gctgtccgcc gcggctgtgg ctgtggctgt ggctgtggca ccgaccggca
    15001 tcacgccggg cccgccgcca tagacaccgg tcttgccggc cttcttctcg ccctccgtca
    15061 ggcaatcgcc gggttcgcgg ctgaagggcc agagcgtccc cttgttgcag ttaaccgtcg
    15121 tggcggtccg gctcgccggc ccaccgccaa aaaccgaagt ggaggtgatc agagccccat
    15181 cgccgctgtt gttgccgctg cttccgctgc cacttccgaa aagagagccg ccgaacagag
    15241 aaccgcccaa aagtccgccg ctgttcgaat cattgttgtt ctgaacggga ggctccgacg
    15301 gcttgacggt gctgaggtcg acattggtgt tgaccggtcc attataaacg ccggtcttgc
    15361 cggaggcgat ttcgacatcg gtcaagcaat cgcctgccgt ccgtacaaag ggccacagca
    15421 tccccttgtt acagtctgcg gccaaggccg agccagttcc ggcaaagcca gccgcgccgg
    15481 acaatgcgac acacgcgaca agtgaaataa tacgcatctc tttatcccgc cgctgttttc
    15541 gtgaagggca cgcaacacgc aacaccatcg gcatttatac tctcagggca ccaatgcgca
    15601 atgcgcccgc cttaccgatg cttctggcca acgtgttagt gatagattga agattcgagg
    15661 tcattgcatg attcaaaaga aaaaacgcgt ggctccgctg ctggcggcgg cggccctctt
    15721 cgggggattc gcgccggcat gcatggcgga cgccgcggag gattccgcgg gcatcgcgca
    15781 ggaactggca ctcgaaccgc cgcaattcga tctcggcgac atcaatgtga agcttggcgg
    15841 attcgcggga gccgcgttct tcaacgcgca tcaatcggcg ggtccggcct atggcagcta
    15901 tgatttcgcg cgcacgagcg gtgaagcgcg cgcgaacatc cgcgcccagc gcattttcga
    15961 caacggcctg atcctgggcg cgggcggcga tttcctgctc tatcacgaca gtctgtccgg
    16021 cgatcaatat ggcaatgatt tcgtcgagaa agccttcctg ttcgcgcaga ccggttttgg
    16081 ccgcgtcgag atcggcgaag cggacggcgc cggctatacg ctcggcctga ccggtcccgt
    16141 caccaatccg gaagtgacgc tggagaatcg caacatctcg ttgtttcacg atccggtcgc
    16201 gggcgaggat ttcgcccgga tcttcgaagc cgtcgccgcg gtgcagagca catcgaattt
    16261 cgccaagatc aattatctca cgccgcgtct gttcggaatc cagatcggcg cgtcattcac
    16321 gcccgaaacg cttcgcgcgc cgctgccctt taccggcaat cccagcaacc tatcggatcg
    16381 gcagcacgac atctgggaga tcgcggcgag ctatacgggc tatgtgtcga acgtcgcact
    16441 gggtttgtcg gcggcttatg cgcatggatc gctcaagaac gaaagtgcgg gcgcggacgc
    16501 ggacggtctc tatgattggt cactcggcgc gcaggcggcc accatgctgt ccgatgtgag
    16561 gctctcgttc ggcggcggct accgcgagag caacgcctat ctcctggaga ccgataccgt
    16621 gctcgcccat ggcggcaccc acaccggcca catctcggcc accgcggaat ggaagcgatg
    16681 gatcgcgggc gtggaatatt ctctttccga aatcagcggc ccggtggatt tcgacatcag
    16741 gggctatcag gtctcggccg gataccggct gaacgacaat ctgcagctca ccgcaggctg
    16801 gcaatggtac gactaccgcc gcaattttgg cacgttctac aacggaggtc ccggcatcga
    16861 catgaatgcc gggtttcttg cccttagcta cgaattgtga gcgggatcaa gagtgagccg
    16921 ggatcagggc ctcgatcgcg gcaattcccg caagccgcgt cccggccaaa cgctgaacgt
    16981 tcgtcgcgtt gacgccgcca agcgcgtaaa ccggcaagcg cgcgctccct gccagcgtac
    17041 aaaagcgcga gacgccaaag ggctcgcgcc cgatatggct ttgggtcgca aaaacgggcg
    17101 cgagcagaac cgcatcgcag cgcgcgcgcg ccgcaacgcc gaggccgcgc cgcgaatggg
    17161 ccgacgccgt gatcagccaa tcggggtgca acgccttcca atggaccgct tcgccggcgc
    17221 gcgcttccgg cagatgcagc ccgtcgcacg ggaggcgtgc ggccagaccc gcatcgcccg
    17281 caatcagcaa tcgcaagccc cgctgccggg cgattttgtg caaagcgcgc gccagcgcag
    17341 cgcgcggcgc ggcctcggtg tgccgcagga tgatcgcagc ccccttgggc agagcgcgcg
    17401 cggcggcgag cgggtcggcg agccgggtct ggtcggtcat caaaatcagc ggcggcagat
    17461 ggggagcagc tgcgttgagc aggcgcgcgg cccttgctag tttcacgcgc gccaacaccg
    17521 atgccatgat gtgacctccg aaaatcctcc catatccgtc gatccggccg ggaatctcaa
    17581 ggccgttctc gcccgcatcg cggccgcgcg ccggtgcgcc atcgcaccgg cggacgaaac
    17641 ccatctgatc gcggtgagca aaacccatga cgaggcgcgc atcctgcccg tcctcgaggc
    17701 cggccaccgc atcttcggcg aaaatcgcgt acaggaagcc aaaagcaaat ggccgcaatt
    17761 gcgcgcgcgg tttgcggatg tgacgctgca tctgatgggg ccgctgcaga ccaacaaggt
    17821 ccgcgatgct gtggcgatct tcgacgccat tcattcgctc gaccgcctga agcttgccga
    17881 ggcactcagg gacgaatgcg cgcgcaccgg caagcgcatc gcgctctttg tcgaagtgaa
    17941 tatcggcgcg gaaacccaga aggccggtct cgcgccgcat gaaaccgcgc gcttcgtgcg
    18001 ccactgccgc gatgggctga acctgaatgt ggtggggttg atgtgcattc cgcccgccaa
    18061 ggaggagccc gcgcttttct ttgctctgct gcggaaactg gcgcgcgaat gcgggcttaa
    18121 cgccctcagc atgggcatga gcgatgattt cgaaaccgcg atccgcttcg gcgccactca
    18181 tgtgcgcgtc ggcacggcga tcttcgggga acgtgaaacc tgattgtccg gcgatctatt
    18241 cgatgatggt catgcgcggc gcgtaacggt gcagcatcgc gccatgttcg gacagccaag
    18301 cctgactctt ctcgatgtcg ggcaccagaa gctcgaccag acgccagaag cgcggccgat
    18361 gattgagttc gcgcaaatgg gccacttcgt gggccacgac gtaatcgagc acggccggcg
    18421 gcgccagcac cagccgccac gagaaggaga gcgagcgcgc ggaggagcac gaaccccagc
    18481 ggctggacgt gtcgcgaatg gtaatccgct tgggacgcac accaagcctt gcggcatgtt
    18541 cggcaacccg ctcgtcgatc ttcatccgtg cttcacgctt caaccagtcc aggagccggc
    18601 gcgacgcgtg ttcaggccgg ccgccgacac ggatggtggg acgcgcggcc tcgcgatcga
    18661 tccagaccgg cgcgcgtttg ccctcgcccg gacggatcgc atattccacg cctttgaaca
    18721 aaatcgtcga gccgatatcg atctgcaccg cgtcgggcac atcggcgagg cggcccgcga
    18781 tccaatcctt ctccttgcgt gcgaaatcga gcgcgcgatc aaaggaacgc gacgatggag
    18841 acaccaccga cacttcaccc gtcgcgggat cgaccttcac gatgaagcgg cgcgcgcgcg
    18901 gattggtgcg cagaatcact tcgatggatt tgccgtcgat cttcaacaat tcgcgtttga
    18961 tgcggcgcgc cggttttgcg ttcgcctcgt cgtgaaattt tttgcgcact gcgatcatga
    19021 attgtccccg acgaaacacg attggtctcg tgtcctatca agccaaattt ttcgtagcgc
    19081 cgttgcggtc cgccatcccg gtacgtcctg cggccccttc ggcacccgcc attaaacctt
    19141 gccttccggc gcgcacaagg gcaaaattta agttcccgct aagtttatta gttgattgaa
    19201 aaataagcat ttttcgattc caatgagaga aattaacgtc tgttgttcat tattttgaac
    19261 gatgatcgga aattttccca aattgcacgt ctttagcggc cctggcatac agggcgggaa
    19321 accccattcg ccgctgccat ggcacttgca atcggcaaag atcacgctct ctcgggatcc
    19381 gccggaacgg acgccgccct ctttgtgggc gcggtgagcg ccgccctgct tctgggcgca
    19441 ttcgggtcgc aatatctcgg cggcctggct ccttgcgaga tgtgcatctg gcaacgctgg
    19501 ccgcacggag cagcgatcct tttggggctt atcgggggag gactggtcag aggcggagtc
    19561 ctgcccccca atcttggccg gaccctggcc attctggcca ttctggccat cgcgatttcg
    19621 ggcgccatcg gcgtcttcca tgccggggtg gaatggaagc tctggccggg ccccgcggaa
    19681 tgcaccgggg tcggttatgt tccgggacgg gacgatttca agccgctgca gatcgtacgc
    19741 tgcgatgagg cgcaatggcg gctgtttggc atttcgctgg ccggctataa tgccgtgttc
    19801 tcgctggcgg ccgctgcctt ggcggcaagc tggctgcggc gggcgcgggc atgaattccc
    19861 gtacgatgag tccctcggca agaacggcaa atcggggggc gcggccggtt cttccgggaa
    19921 cccaggacgc ttgcgccgcc gatatcgaag ccatgatccg tgtcgatcat gcgggcgaat
    19981 tcggtgcgct tcgcatctat gaaggtcagc tcgcggtgct gtcgcgcaaa accccggcct
    20041 ccgaggcaca aagcgatgtg cagtctatcc gtcacatggc ggcccaggaa cagacgcact
    20101 tcgatacctt cgagcgcatg atcgaggaac ggagcgttcg cccgaccgcg ctcgaaccgg
    20161 tctggcggat cgccggcttt gcgctgggtg ccgccaccgc gctgatgggc gagaaagcgg
    20221 cgatggcctg cacggtcgcg gtagaagatg tcatagacga acattattcc agacagatcg
    20281 aacgtcttgg cgaaaacgag ccggatttga aacagacgat ccagaaattc cacgccgaag
    20341 aatgtgccca tcgcgacgaa gcgctggaac gcggcgcgcg cgaggcaccg gcctatccgc
    20401 ttctttccgc cgcgatccgg ctatcctgcc gcatggcgat cgcgctatcg gagcgactat
    20461 gaacgcaagt gcgcaggccc cgatccggcg cgcgacagag gccgacgtgc cgcgtctggc
    20521 acggcttttc gcggcggcct tcgcacgcga tcccctgttc gattggctgg cgcgagcggg
    20581 aagcaagcgc cagcgcgcgc tgcagcgatt tttcggatgg gtcttggctc gccgtaccgt
    20641 gccccacggc gaaacctgga tttccgccga tgggttggcg gcggcggcgt ggataccacc
    20701 ctatgtgaag tccgcgccgc cgcgcttttt ggaagatctg cgcacgcttc cggcgattct
    20761 gcgtctgacg ggtttgaagc gcctgccgcg cggggccgca atggcggctg cgatggacga
    20821 ggctcctccg caaacgccct atttctatct ggctttcatc ggtgtcgcac cgcgcatgca
    20881 gggcgcaggc ctcggctcgg cacttttgcg tcaaacgctg gcgcgggccg acgcggcggg
    20941 cagcggtgcc tatctggaaa attccaatcc gcgcaatctg aagctctatc agcgcgcggg
    21001 ctttgcggtt ctgcgcgagg tcaaggcgca cgccgatgcc ccgcccgtca ccgcgatgtg
    21061 gcgcccggcc aaagcccgtc agagcggcat cccttcagat tgaacgcgat accgctctag
    21121 atttttattt taccgcgcaa attttagggc gaacggcctc acgcgcttgc gcgcgatatc
    21181 aaacgatcgc ggcgaagccg caagggcact tcctatcgcg gcgaagccgc aagggcactt
    21241 cggccaattg cgttttaggg ctcaagcacg atgtcccaat agagatagtc cgcccaggac
    21301 tgatgcagat aattgggtgg aaacatgcgg ccattgtttt gcaactcggc gaccgatggc
    21361 ttatgcggag aattgcgcgg atgcatgccc gcgcgatggg gcaagcggcc gcccttcatc
    21421 agattgcagg ccgcgcaggc cgcgaccaca ttgtcccagg tcgtgcggcc gccacgcgag
    21481 cgcggaatca cgtggtcgaa ggtgaggtct tccttcgatg gacaatattg gcattggaag
    21541 cggtcgcgca ggaaaacatt gaaccgcgtg aaggcgggat tgcgcgtagg ccgcacatag
    21601 gtcttgagcg acaccaccga gggcaggcgc atctgaaagc tcgccgagcg caccgtgcgt
    21661 tcatattcgg ccacgatgtt cacgcgctcg aggaacaccg ccttgatggt gtcctgccag
    21721 gaccatagag acagcggaaa atagctcagc gggcggtaat ccgcgttgag cacgagggcc
    21781 gggcaattgt cggggacacg ctcggcggct agcacgtttg cctatccgta ctgaaggcgt
    21841 cactctccct tgaggcgcga ggcgaaccaa aagcgggcgg gactgtagcc ggaacatcgg
    21901 cgttgcgcca acccttagcg
//
LOCUS       DASYEG010000051         1884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4074322, whole genome shotgun sequence.
ACCESSION   DASYEG010000051 DASYEG010000000
VERSION     DASYEG010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4074322"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VGM72_02045"
     CDS             <1..849
                     /locus_tag="VGM72_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEY4264073.1"
                     /translation="LFFLHGARLSRDTVMAGITHWRLHLLVFASTFILFPSLGMLIGL
                     WLPRLLTPTLYTGILFLCVLPSTVQSSIAFTSIARGNVPAAICSASASNIVGIFLTPL
                     LVGVLLSGKRGVGFSFSEVETILLQLLLPFIAGQICYRWLSGWLGKHKSLTSIVDRGS
                     ILMVVYLAFSNAVVGGLWHQLPLGAIGMLVLVDMAILAIVLAATLIASRVMGFAKEDE
                     ITIVFCGSKKSLASGIPMANAIFAGQNIGAVVLPLMIFHQIQLLVCAVVARRYAERQK
                     AQATAV"
     gene            1047..>1884
                     /locus_tag="VGM72_02050"
     CDS             1047..>1884
                     /locus_tag="VGM72_02050"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY4264074.1"
                     /translation="MNILSRQSRGVIPIVPTPFDDEGRVDLASIPKLIAYYQACGVIG
                     LTILGVMGEASKLTQGETEDVVETFVSHAKGRLPVIVGVSGTSLAASAALALWSQERG
                     AFGVMLQAMSGLQGDDALVTYFEMFAERTQKQCPICVQDYPVSSGVHMSVAAWRRIAK
                     LESVFMLKHEPPAGLQKLSQIRASEKEGACPRIAILTSNNAMHLSQELDRGADGAMVG
                     VAYTDLIVLICRLYAEGQVEKALDIYDAVLPLIRHESQGAFGLAIRKEILRRRGVLRT
                     GHV"
BASE COUNT          320 a          585 c          565 g          414 t
ORIGIN      
        1 ttgttcttcc tccacggcgc ccgcctgtcg cgcgacaccg tgatggccgg catcacgcat
       61 tggcgcctgc atctgctggt cttcgccagc accttcatct tgtttccgag cctcggcatg
      121 ctgatcggct tatggctgcc gcggctcctc acgcccacgc tctataccgg gattctattc
      181 ctctgcgtgc tgccatcgac ggtgcaatcc tccatcgcct tcacctcgat cgcgcgcggc
      241 aacgttccgg ccgcgatctg ctcggcttcg gcgtccaata tcgtcggcat attcctgacg
      301 ccgcttctgg tgggcgtgct gctcagcggc aagcgtggcg tcggcttttc cttctcggaa
      361 gtcgagacga tcctgctgca attgctgttg cccttcatcg ccggccagat ttgctatcgc
      421 tggctttccg gatggctggg caagcacaag agcctgacca gcatcgtcga tcgcggctcg
      481 atcctgatgg tggtctatct ggcgttcagc aatgcggtgg tcggcgggct ttggcaccag
      541 ttgccgctcg gcgccatcgg tatgctggtc ctcgtcgata tggccatcct ggctatcgtc
      601 ctcgccgcca ccctgatcgc cagccgggtg atgggctttg ccaaggaaga tgaaatcacc
      661 atcgtcttct gtggatcgaa gaagagtctc gccagcggca ttccgatggc gaatgcgatc
      721 ttcgcgggac agaatatcgg cgcggtggtc ttgccgctga tgattttcca tcaaatacag
      781 cttttggtct gcgccgtcgt ggcgcgccgc tatgcggaac gccaaaaggc ccaggccacc
      841 gcggtgtgag accatgtggg tcatcacgtt tcgcgataat tataatcggg aatgctttgt
      901 tccgcatgcc cccctacttc ggtatacctt caagatgacg cgctcttttc agcccgacat
      961 cgatccgaaa cgacgcgttg gcgtcggttt ggcgcggttc ttcgcgcgct cagcgaataa
     1021 caacccgatc tcctgagagc catcccatga acatcctttc ccgacagtcg cggggcgtga
     1081 tccccatcgt tccaacgccg ttcgatgacg agggtcgggt cgatctcgcc agcattccga
     1141 agctgatcgc ctattaccag gcgtgcgggg tgatcggatt gacgatcctc ggcgtgatgg
     1201 gagaagcctc gaagctgacc caaggcgaga cggaagacgt cgtggagacc tttgtttcgc
     1261 atgcaaaggg caggttgccg gtcatcgtcg gcgtcagcgg caccagcctc gcggcctcgg
     1321 ccgcgctggc gctgtggagt caggaacgcg gcgctttcgg cgtcatgctg caggccatga
     1381 gcgggctgca gggagacgac gcgctcgtca cttatttcga gatgttcgca gagcgcacgc
     1441 aaaaacaatg tccgatctgc gttcaggatt atccggtctc cagcggcgtc catatgtcgg
     1501 tggcggcgtg gcgccgaatc gcgaaattgg aatcggtgtt catgctgaag catgagccgc
     1561 cagccgggct gcagaagctc tcccagatcc gcgcgagcga gaaggaaggc gcctgcccgc
     1621 ggatcgcgat cctcacctcc aacaacgcga tgcatctctc gcaggaactc gatcggggtg
     1681 ccgacggcgc catggtcggc gtggcctata ccgatctgat cgtcctcata tgccgcctct
     1741 atgccgaggg acaggtagag aaggccttag acatctatga cgcggtgctt cccctcattc
     1801 ggcatgaaag ccagggcgcc ttcggtctgg cgatccgcaa ggaaattctg cggcggcgcg
     1861 gtgtgcttcg caccggtcac gtcc
//
LOCUS       DASYEG010000052         2564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4656324, whole genome shotgun sequence.
ACCESSION   DASYEG010000052 DASYEG010000000
VERSION     DASYEG010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4656324"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..823
                     /locus_tag="VGM72_02055"
     CDS             89..823
                     /locus_tag="VGM72_02055"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264075.1"
                     /translation="MAFTSWRGVVGNIKPTMRPGSVEELCRILPEGVGIIPLFLDIQK
                     GAREEFTRIIPHYEPLIAKLAEHEVDLIHPEGAPPFMVLGYEGEAELIKKWEKKYKTP
                     IFTSGTNHIRALNALKIKRFVGASYFSGKINETFGRYFVDAGFDVLGMEGIDVPFHDV
                     GNLSGELVYAHVKKIFLKHRKDAEGIYLLGSGWRVMGIIDMLERDLGVPVIHPVPARC
                     WEIQKRLMINHPVAGYGRLLSEMIAG"
     gene            820..1848
                     /locus_tag="VGM72_02060"
     CDS             820..1848
                     /locus_tag="VGM72_02060"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY4264076.1"
                     /translation="MRFRWAACALGVAMMGVLAAMPARAAAPQPFYKGKQIILLIASG
                     AGGGYDAYARALARHMGPHIPGNPAIVPKNTPGAGGLIAANTLYNSSAPDGLTFAALT
                     NGAAMDPLFGERAARFDGQKFAWLGSIGKLENICVTWKGKSPITRIEQARDKEVTVSA
                     TGATGNSAIMPKIVNQFLGTKFKVIGGYAEGAGQTLALERGEVDGICGLSYSTLKAAH
                     PDYFRDNKLNIILQIGLEKLKDLPNVPNAIDLVSDPDSKKVLELILIRQEMGRPFALP
                     PGTPADRVAILRAAFDATLKDPAFLAEAAKLQLEIDPLTGPQIEDLLKTAYAAPKPIV
                     ARAAQLTK"
     gene            1972..>2564
                     /locus_tag="VGM72_02065"
     CDS             1972..>2564
                     /locus_tag="VGM72_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY4264077.1"
                     /translation="MNRHYDYIIAGAGSAGCVLANRLSEDSTARVLLLEAGGPDRHPY
                     IHIPLGLGMMHKKRMFDWGYDSEPEPALNGRRIEAARGKVLGGSSSINVMAFTRGDPG
                     DYERWAQSGALGWSNAELLPYFKRVETWQDGENAFRGGHGPMGVEWAKTTDPLFDAWI
                     EAAKAAGFPHVQDYNAGSHEGFGRSQYSIRNGRRSSSA"
BASE COUNT          510 a          785 c          795 g          474 t
ORIGIN      
        1 tgaagcgcac ctgagaaagc gcctggaaac cgcctcgcaa accccctaat ttcggccagc
       61 tttacacgaa tccgaaaagg ggacgaattt ggccttcaca tcctggcgcg gcgtcgtcgg
      121 caacatcaag ccgaccatgc gccccggctc cgtcgaggaa ttgtgccgca tcctgcccga
      181 gggcgtgggg atcattcccc tcttcctcga catccagaag ggcgcgcgcg aggaattcac
      241 gcgcatcatc ccgcattatg aaccgttgat cgccaagctc gcggaacacg aggtggattt
      301 gatccatccc gaaggcgcgc cgccgttcat ggtgctcggc tatgagggcg aggccgagtt
      361 gatcaagaaa tgggagaaga aatacaaaac ccccatcttc acctcgggca cgaatcatat
      421 ccgggcgctg aacgcgctga agataaagcg ctttgtcggc gcctcctatt tttcgggcaa
      481 gatcaacgag acctttggcc gctatttcgt cgatgcgggt ttcgatgtcc tcggcatgga
      541 ggggatcgat gtgcccttcc acgatgtcgg caatctgtcg ggcgaactgg tctatgccca
      601 tgtgaagaag atcttcctga aacatcgcaa agacgcggaa gggatttatc tcctgggctc
      661 gggctggcgc gtgatgggca tcatcgacat gctggagcgc gaccttggcg tgccggtcat
      721 ccatccggtg cccgcgcgct gctgggagat tcagaagcgg ctgatgatca accatccggt
      781 cgcgggctat gggcggctct tgtccgagat gatcgcggga tgagatttcg ctgggccgcg
      841 tgcgctttgg gtgtcgccat gatgggcgtg ctcgccgcca tgccggcccg cgccgctgcg
      901 ccgcagccct tctacaaggg caagcagatc attcttctga tcgcgagcgg tgcgggcggc
      961 ggctatgacg cctatgcgcg cgcgctcgcc cgtcatatgg gcccgcacat cccgggcaac
     1021 ccggccatcg tgcccaagaa cacgccgggc gcgggcggat tgatcgcggc caatacactt
     1081 tacaacagca gcgcgcccga cgggctgacc ttcgccgcgc tgaccaatgg cgcggccatg
     1141 gacccgctgt tcggcgagcg cgcggcgcgc ttcgacggac agaaattcgc ctggctcggc
     1201 tcaatcggca aactcgaaaa catctgcgtg acgtggaaag gcaaaagtcc gatcacgcgc
     1261 atcgagcaag cgcgtgacaa ggaagtcacc gtgagcgcga caggcgccac cggcaattcc
     1321 gcgatcatgc cgaagatcgt caatcaattc ctcggcacca aattcaaggt gatcggcggc
     1381 tatgccgaag gcgcgggcca gacgctggcg cttgaacgcg gcgaggtcga cggcatctgc
     1441 ggcctttcct attcgacgct gaaggccgcg caccccgatt atttccggga caacaagctc
     1501 aacatcatcc tgcagatcgg tctcgagaag ctgaaggatt tgccgaacgt gccgaacgcg
     1561 atcgatcttg tctccgatcc cgacagcaaa aaagtgctgg agctgatcct gatccgtcag
     1621 gagatgggcc ggccgtttgc gctgccgccg ggaacgcccg ccgaccgcgt ggcgattttg
     1681 cgcgccgcgt tcgatgcgac gctgaaggac ccggcgttcc tggccgaagc cgccaaattg
     1741 caattggaga tcgatccgct gacgggtcca cagatcgagg acttgctgaa gaccgcttac
     1801 gcagcgccca aacccattgt cgcgcgcgcc gcccaattga cgaaatagaa aaccacaaag
     1861 ggcacgaagc agacacgaag tacgcgaagg catttggacg aagcttcgtg accttcgtgc
     1921 gccctttgtg ccctttgtgt ttgactttac cgacctgccg cggggctgaa tttgaatagg
     1981 cattacgact acatcattgc cggtgcggga tcggcggggt gcgtgctcgc caaccgcctc
     2041 tctgaggaca gcaccgcgcg cgtgctgctg ctggaagccg gcgggcccga caggcatccc
     2101 tatatccata tcccgctggg tctcggcatg atgcacaaga agcgcatgtt cgattggggc
     2161 tatgacagcg agcccgaacc cgcgctcaat ggccgccgca tcgaggccgc gcgcggcaag
     2221 gtgctcggtg gttcgtcttc catcaatgtg atggcgttca cgcgcggcga tcccggcgat
     2281 tacgaacgct gggcgcaatc gggcgcgctg ggctggtcca atgccgaatt gctgccctat
     2341 ttcaagcgcg tcgagacctg gcaggatggc gagaacgcgt ttcgtggcgg acacggtccc
     2401 atgggcgtcg aatgggcgaa gaccacggac ccattgttcg atgcctggat cgaagcggcc
     2461 aaggcggcgg gcttcccgca tgtgcaggat tacaatgccg gttcgcatga aggctttggc
     2521 cgcagccaat attccatccg caacggacgg cgttcgtcct cggc
//
LOCUS       DASYEG010000053         2323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3641511, whole genome shotgun sequence.
ACCESSION   DASYEG010000053 DASYEG010000000
VERSION     DASYEG010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3641511"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VGM72_02070"
     CDS             complement(<1..276)
                     /locus_tag="VGM72_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEY4264078.1"
                     /translation="MAETSYTPAPDADFVLPFDLPDVGLRGRILRLDAVSTRALAAHP
                     LPEAGQRVLAEALALSALLGSSLKFEGRLSVQTKGDGPLNLLATDYFA"
     gene            complement(320..1285)
                     /gene="argF"
                     /locus_tag="VGM72_02075"
     CDS             complement(320..1285)
                     /gene="argF"
                     /locus_tag="VGM72_02075"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEY4264079.1"
                     /translation="MTASGALKQHKPTASGPRHFLDLADLDSATLNGLIASARRRKDD
                     RAGFPRGLKDADVPLDGRMLALIFEKPSTRTRISFDVGMQELGGSTIMLTGAEMQLGR
                     GETIADTARVLSRYVDAIMIRTLGHDTLTQLAEYAGVPVINGLTKLSHPCQVMADVMT
                     FEEKKGPIRGSTIAWTGDCNNVCASWIHAAARFDFTLHVATPRELGPRPLLRKWAENS
                     GAKIVFTGDAAEAVAEADCVVTDTWVSMGDKEGTKRHNLLKPFQVNAALMARAKPDAI
                     FMHCLPAHRGEEVTDAVIDGPQSVVFDEAENRLHIQKAILLWCLA"
     gene            complement(1282..>2323)
                     /locus_tag="VGM72_02080"
     CDS             complement(1282..>2323)
                     /locus_tag="VGM72_02080"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY4264080.1"
                     /translation="HLVSVLREQAGKLWHTSNLYRVPGQERLARRLIDASFADTVFFA
                     NSGAEAVECAIKMARRFHYASGAPERFRLLTFEGAFHGRTLATIAAGGSPKYLEGFGP
                     KVEGFDPVPLGDFDAVRNACGPQTAGILIEPIQGEVGVRAAAPRFLKRLREFCDAQGL
                     LLVLDEVQTGIGRTGRLFAYEDAGIVPDIMAVGKGIGGGFPLGACLATERAAKGMTLG
                     SHGSTYGGNPLAMAVGSAVLDLVLEPAFLEHVRAISGSMQQHLAMLAAEHRGVIAEVR
                     GRGLLLGLRLHVPAAAFIAKLRENRLLAVGATENVARLLPPLNIEERHVKEAMRALMT
                     ACAAFETERQAV"
BASE COUNT          437 a          754 c          742 g          390 t
ORIGIN      
        1 cgcgaaataa tcggtcgcca ggagattgag cggcccatcg cccttggtct gcacgctgag
       61 acgcccctcg aatttcagcg acgaacccag caacgccgac aaggccaggg cctcggccag
      121 cacgcgctga ccggcctccg gtagcgggtg ggccgcaagc gcgcgtgtcg aaaccgcatc
      181 cagacgcagg atccggcccc gcagacccac atccggcaga tcgaatggaa gaacgaaatc
      241 ggcgtcaggc gcaggtgtgt aggatgtttc ggccatgggc caatgtgggg tcggatttgg
      301 cgccccgcaa gagcaagcct taagccaggc accagagcag aatggccttc tggatatgga
      361 gccggttctc cgcctcgtcg aacaccaccg attgcggccc gtcgatcacc gcatcggtga
      421 cttcctcgcc gcgatgggca ggcagacaat gcatgaagat cgcgtcgggt ttcgcgcgcg
      481 ccatcaacgc tgcgttcacc tgaaacggct tcaggagatt gtgacgcttg gttccttcct
      541 tgtcgcccat ggagacccag gtatcggtga ccacgcagtc cgcctccgcg acagcctctg
      601 cggcatcgcc ggtgaaaacg atcttcgccc ccgaattttc cgcccatttg cgcagaagcg
      661 ggcgcggccc gagttcacgg ggagtggcca cgtgcaaggt gaaatcgaaa cgggccgccg
      721 catggatcca ggacgcgcac acattgttgc agtcgccggt ccaggcaatc gtgctgccgc
      781 gaatgggtcc ctttttctcc tcgaacgtca tcacatcggc catcacctga caggggtggg
      841 acagtttggt caggccgttg atcaccggaa cgccggcata ttcggccaat tgcgtcagcg
      901 tgtcgtggcc cagcgtgcgg atcatgatcg catcgacata gcgcgacagg acgcgcgccg
      961 tatcggcgat ggtttcgccg cggccgagct gcatttcggc gcctgtcagc atgatggtgc
     1021 tgccgcccag ttcctgcatg ccgacatcga aggaaatgcg cgtgcgggta gagggcttct
     1081 cgaagatcag cgcgagcatg cgtccgtcga gcggcacatc cgcatccttc agcccgcgcg
     1141 ggaaacccgc gcgatcgtcc ttgcggcggc gtgccgaagc gatgaggccg ttcagcgtcg
     1201 cggaatccag atcggcaaga tcgaggaaat gccgtggacc gctcgccgtg ggcttgtgct
     1261 gtttgagagc cccggacgcg gtcataccgc ttgcctctcg gtttcgaatg cggcgcaggc
     1321 tgtcatgaga gcacgcatcg cttccttgac gtggcgttcc tcgatattca aaggcggcaa
     1381 cagacgcgcg acattctcgg tcgctccgac cgcgagcagc cgattttccc gcagcttcgc
     1441 gataaacgcc gctgcgggaa catgcagccg caatcccaac agcaggccgc gcccgcgcac
     1501 ctcggcgatg acgccgcgat gctcggccgc cagcatcgcg agatgctgct gcatggagcc
     1561 ggatatcgcg cggacatgtt cgaggaaggc cggttccagg acgagatcca ggacagcgct
     1621 tcccaccgcc atggcgagcg gattgccgcc ataggtggac ccgtgcgagc ccagtgtcat
     1681 gcccttggcg gcgcgctcgg tggcgaggca ggcgcccagc gggaaaccac cgccgatgcc
     1741 tttgcccacc gccatgatgt cgggaacgat cccggcatcc tcataggcga aaagccggcc
     1801 ggtgcggccg atgccggtct gaacctcgtc aagcaccagg agcagtccct gcgcgtcgca
     1861 gaactcccgt aagcgcttca gaaatctcgg cgcggccgcg cgcacgccca cttcgccctg
     1921 gatcggttcg atcaggatgc cggccgtttg cggtccgcag gcgttcctga cggcatcgaa
     1981 atcgccgagc ggcacgggat cgaacccttc gaccttcggt ccgaagcctt cgagatattt
     2041 ggggctgccg ccggccgcga tcgtggcaag cgtgcggcca tggaacgcgc cctcaaaggt
     2101 gaggaggcga aaacgctcgg gcgcgcccga cgcatagtga aaacggcgcg ccatcttgat
     2161 cgcgcattcg accgcttccg cgccggaatt ggcaaaaaag accgtgtcgg cgaaactcgc
     2221 gtcgatcagg cggcgcgcca ggcgctcctg accgggcacc cggtagagat tggaggtatg
     2281 ccagagtttg cccgcctgtt cgcgcaacac cgaaacgaga tgc
//
LOCUS       DASYEG010000054         5933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1322980, whole genome shotgun sequence.
ACCESSION   DASYEG010000054 DASYEG010000000
VERSION     DASYEG010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322980"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VGM72_02085"
     CDS             <1..426
                     /locus_tag="VGM72_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264081.1"
                     /translation="NLIKRAPDRVVAGVLAQPVGFRPEMPTLLYDGGMTVWGPELMKQ
                     RPNITKDTVEKFLTKMFRSNADFVFTVSRDFVRNCQTPVLILPDDIPAHPYAVAMEAA
                     MLAPKAEVSVYPWKEPKDGIPLAVRQIRSFMKAHRPASV"
     gene            561..878
                     /locus_tag="VGM72_02090"
     CDS             561..878
                     /locus_tag="VGM72_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264082.1"
                     /translation="MTKRKKPAADPDDVLAELLIECRDGVRTCFELSHDPEWDDRGRC
                     EAMNVAARLMKTSLALAGALKKKPDFTHRIIVERAAAIPPPPNFPAKQFMAVRNRKDR
                     KTE"
     gene            1075..2046
                     /locus_tag="VGM72_02095"
     CDS             1075..2046
                     /locus_tag="VGM72_02095"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY4264083.1"
                     /translation="MRVLVLHSDVPPDAPPDDQDTLLQATAIESTLAELGHESSCAVF
                     TPDPAALDGLIARECPDLVFNLVEVVWGRGLYAPLAPQMLSQMGVPFTGASAAAIAVC
                     GDKVLSKRLLKGAGLPTPAWSEPPHWPGIGTGRWIVKSTSEDASLGLDDGAVVSGREA
                     VVMRAQSSAARHGGRWFAERYIDGREFNVALIEKDGAPQILPIGEMTFERWDENRPRI
                     IGYAAKWDEAAADYRDTARVFVWSEREPDLHEKLERLSKECWTLFGLSGYARVDFRID
                     AHGEPYILEINPNPCLEPNAGVAAAAEQADVPYAALIDDIVHAALRG"
     gene            2039..2521
                     /locus_tag="VGM72_02100"
     CDS             2039..2521
                     /locus_tag="VGM72_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4264084.1"
                     /translation="MAEGFRSTPRQSDVDAVERLVRLTHVFNEGEVAIARELVQESLA
                     KGAQASGYYFLFADGPGVVAGYACFGPIAGTAGRWELYWIAVDPGAHRSGLGKRLQGA
                     SEEAVRAFGGRLLIAETSTRPDYAPARGFYLSQGYKLLAEVPDWHDDGDGLAIFGKRL
                     "
     gene            complement(2518..3429)
                     /locus_tag="VGM72_02105"
     CDS             complement(2518..3429)
                     /locus_tag="VGM72_02105"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY4264085.1"
                     /translation="MSAPTHRDRPLVGIGCKVAATFLFAVMFAGIRWLGPYFPLGEIV
                     FFRSVLGIPVIVAMAYATGGLRLLATARIDSHALRSIAGTVAMFCNFAAYTYLPLADA
                     TAIGFAAPLFVVILAALMLSERVHVTRWSAVLVGFIGVVIIAGPEAGLSRSALYGAIF
                     ALTGAGLTAVAMIFLRRMSAHEHSITIAFYFMLTSAAVSLTTLVFGWNWPTGAEAAVL
                     ILTGLAGGVGQLFLSFSYRYGEASVLAPFDYAAMIWAVVLGYFVFAELPGPQVWFGGT
                     IVIAAGLLILWRERQLGRARIAGALES"
     gene            complement(3439..4410)
                     /gene="corA"
                     /locus_tag="VGM72_02110"
     CDS             complement(3439..4410)
                     /gene="corA"
                     /locus_tag="VGM72_02110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEY4264086.1"
                     /translation="MLKAYQQQNGGLKGVTITEGEALPVGALWLDMLNPTLEERRKVD
                     KFLGLELPTRADMEEIEISSRLYQEDTTLFMTAMVMAQTDTDLPTADAVTFVLTAERL
                     VTMRYIDPQPFRTYALRCERSVINAGRAEFVLMQILDAIIDRMADILEKTGSDVEIIS
                     GEIFSPVSHKMGTRDFQGILRRLGQKHGLTSKMRESLLTIMRMLTFLTQSVDNKHNKE
                     TRAHVKTLVRDVQSLQDHSSFVATKVSYLQDGTLGLINNEQNNIIKIMSVASIVFLPP
                     TLFASIWGMNFHHMPELDETWAYPMAIVVMIISGILPYIYFKRRGWL"
     gene            4608..5612
                     /locus_tag="VGM72_02115"
     CDS             4608..5612
                     /locus_tag="VGM72_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEY4264087.1"
                     /translation="MTAMAAPIPDFADVQRAAARLNGVAVMTPLLESPFLSERAGGRV
                     FLKLETLQRTGSFKFRGAYNRLVQLTPDERSRGVVAFSSGNHAQGVASAAALLQMPAT
                     IVMPADAPRIKTQSTRRLGATVVAYDRRRESREEIAARIAAETGAVVVPSFDDPHIVA
                     GQGTAGLEIAGQAAAQGVTLDAVIVPCSGGGLSSGIALALSGASAGTRLFTAEPEGYD
                     GARLSLEGGIRTTAPGTRETIADALTSPGVGAIPFAILKSCGAYGLAVTDEAMMRAVG
                     MAALQLKLVVEPGGAAALAAVLSGTFDARGKSVAIVISGGNIDPETLTRCMDVSLPRS
                     "
BASE COUNT         1091 a         1824 c         1943 g         1075 t
ORIGIN      
        1 aatcttatca agcgcgcacc ggatcgggtg gtcgcgggcg tgctggcgca gccggtcggt
       61 ttccgtcccg agatgcccac gcttttgtac gatggcggca tgacggtctg gggccccgaa
      121 ttgatgaagc agcggcccaa catcaccaag gatacggtcg agaaattcct caccaagatg
      181 ttccgcagca acgccgattt cgtcttcacc gtgtcgcgcg atttcgtgcg caactgccag
      241 acgccggtgc tgatcctgcc cgatgacatc ccggcccatc cctatgccgt cgccatggag
      301 gccgcgatgc tcgcgcccaa ggccgaagtg agcgtctacc cgtggaagga gcccaaggat
      361 ggaattccac tggcggttcg ccagatccgt tccttcatga aggcccatcg gcccgcttct
      421 gtttgaaccc gtcgcgcgcg tgtttgcggc gccgcggacg atttatcccc gccggccgcc
      481 gccgattccg ccgaaagggc atgatttatc cacactctcc ggcgcgggcg taaaataccc
      541 gcgcccaatg gagagtgccg atgaccaaac gcaagaagcc cgcggctgac ccggacgacg
      601 tgttggcgga attgctcatc gaatgccgcg acggtgtgcg tacttgtttc gaactgagcc
      661 atgatcccga atgggacgac cggggacgct gcgaggcgat gaacgtcgcg gcgcggctga
      721 tgaagaccag cctggccctg gcgggggcgc tgaagaagaa gcccgatttc acccatcgca
      781 tcatcgtgga gcgcgctgcg gcgatccccc cacccccgaa ttttccggca aaacaattca
      841 tggcggtcag gaaccggaag gatcgcaaaa ctgagtaggg gcgcgccgta cgggaacagg
      901 aatgcggcca aaccggcaag ccgtgcccga agaatgcgcg cactgaagcg ccacgtccgc
      961 gcgttgatcg tccgcgcgcg agaggcgttg gccgcacaga cgtccacaga tcgctcgcgg
     1021 ctttttcgag tgacgccggg atgacaagat cgttaggact gctacactcg aatcatgcgt
     1081 gtgctcgttc ttcattccga tgtgccgccg gatgcgcctc cggacgatca ggatacgctg
     1141 cttcaggcga cggcgatcga atcgacattg gccgaactcg ggcacgaatc gtcatgcgcg
     1201 gtgttcacgc ccgatcccgc tgcgctggat ggcttgatcg cgcgcgaatg tcccgatctc
     1261 gtgttcaatc tggtcgaagt ggtgtgggga cgcgggcttt atgcgcctct cgcgccccag
     1321 atgctcagtc aaatgggtgt gcccttcacg ggggcgtcgg ccgcggccat cgcggtttgc
     1381 ggcgacaagg tgctctccaa gcggctgctc aaaggtgccg gcctgccgac accggcctgg
     1441 tcggaaccac cgcattggcc gggcatcggg accgggcgct ggatcgtgaa atccacgagc
     1501 gaggacgcgt cgctcgggct ggatgacggt gccgtggtca gcggccgcga agccgtcgtg
     1561 atgcgcgcgc aaagctccgc ggcccgccat ggcgggcgct ggttcgcgga acgctatatc
     1621 gacgggcggg agttcaatgt cgccctcatc gagaaggatg gagcgccgca gatccttccc
     1681 atcggagaga tgacattcga gcgctgggac gagaaccggc cccgcatcat cggttatgcc
     1741 gcgaaatggg atgaagccgc cgccgattat cgcgacacgg cgcgcgtctt cgtctggagc
     1801 gagcgcgagc ccgacctgca cgaaaaactg gagcgtctgt cgaaagagtg ctggacgttg
     1861 tttgggctca gcggttatgc gcgggtcgat ttccgcatcg atgcgcatgg cgagccctat
     1921 atcctcgaaa tcaatcccaa tccctgtctc gaaccgaatg ccggggtcgc cgccgccgcc
     1981 gagcaagccg atgttcctta tgccgcgctg atcgacgata tcgtccacgc cgcgcttcgt
     2041 ggctgagggt tttcgcagca cgccgcgaca atccgatgtc gacgcggtgg agcgtctggt
     2101 gcggctgacc catgtcttca acgagggcga ggtcgcgatt gcccgcgagc tggtccagga
     2161 atccctcgcc aagggggcgc aagcctcggg ttattatttc ctgttcgcgg acggtcctgg
     2221 cgtcgtcgcg ggctatgcct gcttcggccc catcgccggg acggccgggc ggtgggagct
     2281 ttactggatc gccgtcgatc ccggcgcgca tcgttcgggc ctgggcaagc gtctgcaagg
     2341 agcgagcgag gaggcggtgc gcgcgtttgg cgggcgcctt ctgatcgccg aaacttcgac
     2401 gcggcccgat tatgcgccgg cgcggggttt ctatctttcg cagggctaca aactgctcgc
     2461 cgaagtgccc gattggcatg acgatggcga cgggttggcg attttcggca aacgtctcta
     2521 gctctcgagc gcgccggcga ttctggcgcg gccgagctgc cgttcgcgcc agagaatcaa
     2581 gagccccgcc gcgatcacga tggtgccgcc gaaccacact tgcgggcccg gcaattcggc
     2641 gaacacgaaa tagccgagca ccaccgccca gatcatcgcc gcgtaatcga agggcgcgag
     2701 cacggaggcc tcgccatagc gataggaaaa gctgagaaac aattgcccga cgccacccgc
     2761 aaggcccgtc aggatcagaa cggcggcttc agcgccagtc ggccagttcc agccgaagac
     2821 caaagtcgtg aggctcaccg ccgcgctcgt cagcatgaaa tagaacgcga tggtgatgga
     2881 gtgttcatgc gcgctcatgc ggcgcaggaa aatcatcgcc accgccgtca gcccggcgcc
     2941 tgtgagcgcg aatatcgcgc cgtaaagcgc gctccgcgac aggccggcct cgggtccggc
     3001 gatgatgacg acgccgatga agcccacgag aaccgcgctc cagcgggtga catgcacgcg
     3061 ctcggacagc atcagcgcag cgaggatcac cacgaacaga ggggcggcga agccgattgc
     3121 ggtcgcgtcg gcgagcggca gataggtgta ggcggcgaaa ttgcagaaca tcgccacggt
     3181 gcctgcgatg gatcgcagcg catggctgtc gatcctggcg gtcgccagca gcctcaggcc
     3241 cccggtggca taggccatcg cgacaatcac cggaatgccg agcacactgc ggaagaagac
     3301 gatctcgccg agcgggaaat agggtccgag ccagcggatg ccggcgaaca tcaccgcaaa
     3361 taggaatgtc gccgcgacct tgcagccaat cccgacaagg ggccggtcgc ggtgcgtcgg
     3421 ggcgctcatg gagcccctct acagccagcc gcggcgcttg aaatagatat agggcaggat
     3481 gcccgaaatg atcatgacga cgatcgccat cggataggcc caggtctcgt cgagttccgg
     3541 catgtggtgg aaattcatcc cccagatcga agcgaacagg gtcggcggca ggaatacgat
     3601 cgaggccacc gacatgatct tgatgatgtt gttctgctcg ttattgatga gcccgagcgt
     3661 gccgtcctgc agatagctca ccttggtggc gacaaagctc gaatggtcct gcaaggactg
     3721 cacgtcgcgc accagggttt tcacgtgggc gcgggtttcc ttgttgtgct tgttgtccac
     3781 cgactgcgtg agaaatgtca gcatccgcat gatggtgagc agactttcgc gcattttcga
     3841 ggtgagcccg tgcttctggc cgagacggcg cagaatgccc tggaaatcgc gtgtccccat
     3901 cttgtgcgag accggcgaga aaatctcccc cgagatgatc tccacgtcgg agccggtctt
     3961 ttccagaata tcggccatcc ggtcgatgat cgcatcgagg atctgcatca gcacgaattc
     4021 cgcccggccc gcattgatga cggagcgttc gcagcgcagc gcataggtgc ggaagggctg
     4081 cggatcgata tagcgcatcg tgaccagacg ctcggccgtc agcacgaagg tgaccgcatc
     4141 ggcggtgggc agatcggtgt cggtctgggc catgaccatg gcggtcatga agagggtggt
     4201 atcttcctgg tacagccggc tggagatttc gatctcttcc atgtccgcgc gcgtcggtaa
     4261 ttccagaccc aggaatttgt cgaccttgcg gcgctcttcg agcgtcggat tgagcatatc
     4321 gagccagagg gcgcccaccg gaagggcttc gccctcggta atggtcacac cttttaggcc
     4381 gccgttctgc tgctgatagg ctttcagcat gcgacgcttc ccgctgggat taacgaacac
     4441 aaattgcgaa tgaggagcgc ttgaattgga aaagacaacc gcttcagtcc catatagcct
     4501 caagcagcgg ccggcgcgaa ggatatttcg ccgcattcca ggatttcatg ggcacctccc
     4561 ggtttggcct cgccgggccg aaggcgggat aggctaagct ccttttcatg actgcgatgg
     4621 ccgcacctat tcccgacttc gccgatgtcc agcgcgctgc cgcgcggctg aacggtgtgg
     4681 cggtgatgac gccattgcta gaaagcccgt tcttgtccga acgggccggt ggccgcgtct
     4741 tcctcaagct cgaaacgctg cagcgcacgg gctcgttcaa gttccgcggg gcctataatc
     4801 ggctggtgca gctcaccccc gatgagcgga gccgcggcgt ggtcgccttc tcctcgggca
     4861 atcatgcgca aggcgtggcc tcggctgccg cgctgctcca gatgcccgcc accatcgtga
     4921 tgcccgccga tgcgccccgc atcaagacgc agagcacgcg taggcttggc gcgaccgtcg
     4981 ttgcctatga ccggcgccgc gaaagccgcg aggagattgc cgcccgtatc gccgctgaga
     5041 cgggcgcggt cgtggtgccg tccttcgacg atcctcacat cgtcgcaggg cagggaaccg
     5101 ccggtctgga gatagcaggt caggcggcgg cccagggcgt cacgctggat gccgtgatcg
     5161 tgccctgcag cggcggtggg ctttcgtcgg gcatcgcact cgcattgagc ggggcgagtg
     5221 cgggcacccg cctcttcacc gcggagccgg aaggctatga cggggcgcgc ttgtcgctcg
     5281 aaggcgggat tcggaccacc gcgccgggca cgcgcgaaac aatcgccgat gccctgacct
     5341 cgccgggcgt gggtgcgata ccattcgcta ttctgaagtc ttgcggcgcc tacggtctcg
     5401 ccgtgacgga tgaggcgatg atgcgtgcgg tcggcatggc ggctctgcaa ctgaaactcg
     5461 tggtcgagcc gggcggtgct gcggcgctgg cagcggtctt gtcgggcacg ttcgacgcgc
     5521 ggggcaagag cgtggccatc gtcatctccg gcggcaatat cgaccccgag acgctcacgc
     5581 gctgcatgga cgtgtcgttg cctcggtcat gagaaatttg gggaggggac gatgaggccg
     5641 atttgtgtgg ctttcggggt ggccttggcg atcgtggcaa aggcggcgcc catgtgaaaa
     5701 tcacccctct ccgtaaagct gtactgaata gcgtctctct gagtccgcgc cgctgatcgc
     5761 tggtccaaat gaagggattt cttcgggggg cgcccttcag agccacgcgt aaaccatgta
     5821 agcttcttcc atcacctccg ggagttattc atgaacagat tgatggtgcc agcggtgctc
     5881 ctggcaagcg gtgtcgcagc aacggccgca tggtcgcagc cgaagccgcc cgc
//
LOCUS       DASYEG010000055        10399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3207587, whole genome shotgun sequence.
ACCESSION   DASYEG010000055 DASYEG010000000
VERSION     DASYEG010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 10399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10399
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3207587"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..2211
                     /locus_tag="VGM72_02120"
     CDS             64..2211
                     /locus_tag="VGM72_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY4264088.1"
                     /translation="MAKRMASFGMLGVSFAALLAATAPAASQAVDAGDIGGVVTGANG
                     PEAGVWVIAETRDLPTKYVKIVVTDDAGRYLIPDLPTANYDIWVRGYGLVDSPRVKSA
                     TGKMLNLKAVPAPSAAAAAAYYPAIYWYSMLKIPDPKLFPGTGVKGNGMPVSLKDQGV
                     WLRQLKTDGCNSCHQLGDIATRELPAQLGHFDSSAAAWERRIQSGQPGALMVQALGDL
                     DGSRALSLFGDWTDRIKKGELPFAKPVRPAGIERNVVLTMWDWSQPTAYMHDLTSTDK
                     RNPTLNVNGIIYGSPELSSDYVAWLDPANNKTGLIKTEWRDPKTPSTKTSPIYAPSPY
                     WGQEPVWDSHSLNHNPMMDAKGRVWLTSRIRPDANPAFCKAGSHHPSAEAFPLETSRR
                     QLQMYDPATKKITTIDTCFTTHHLNFDRDGKIWFSSGSNTDPVVGWFDTKKWDETHDE
                     QASQGWAPFIVDVNGNGKVDAYVEPNQPLDPKKDKRVLQGLYSVSANPSDGTIWAVVL
                     GFPGTAIRFDPKTKLTEVYEVPWNDPKNPQSGFSPRGADLTSDGVLWSVLASGQMASF
                     DRRLCKGPLTGPVAATGKACPEGWKLYTIPGPQFKGVAMKGGSAEAPYYDWVDQFDTL
                     GLGKNTPIATGNLSDAMFAMVDGKWVTMRVPYPMGFFAKGMDGRVDDAKAGWKGKGIY
                     ATYSGSASTHIEGGKGTMPKVIHFQLRPNPLAK"
     gene            2262..2762
                     /locus_tag="VGM72_02125"
     CDS             2262..2762
                     /locus_tag="VGM72_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264089.1"
                     /translation="MILGMPIATFTAVHVILSLLGIAAGLVVVFGMIAGKHSSAWTGI
                     FLAATALTSVTGFPIPPLGFDPPRAVGVLSLLLLAFAVPALYVFRLAGWWRWIYVVTA
                     TAALYFNCFVGVVQTFQKIAFFKSLAPTQSEPPFAIAQIAILVILIALGALAVKKFHP
                     RMSRAF"
     gene            2763..3596
                     /locus_tag="VGM72_02130"
     CDS             2763..3596
                     /locus_tag="VGM72_02130"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4264090.1"
                     /translation="MSEESFEGAGGLRIFFRSWRPTGMPRGVVVIVHGFNAHSGQYDW
                     VAAQFVARGLAVYAPDLRGRGNSDGERYYVEKFADYVGDVAGLMTIVKSREPRLPVFL
                     LGHSAGGVVSCLYALEHQSDLAGLICESFAFQVPAPDFVLAVLKGISHLAPHAHVLHL
                     KNEDFSRDPKVVAAMNADPLIAHETQPTRTVAEMVHADELLKQDFPLIVLPVFILHGT
                     ADKATRPSGSQLFYDKAGSKHKTLKFYEGYFHDPLHDLGKETVMADIQAWVDKRITPA
                     K"
     gene            complement(4246..6891)
                     /gene="topA"
                     /locus_tag="VGM72_02135"
     CDS             complement(4246..6891)
                     /gene="topA"
                     /locus_tag="VGM72_02135"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEY4264091.1"
                     /translation="MSEPSSVVVVESPAKAKTINKYLGRDYTVLASYGHVRDLPAKDG
                     SVDPNSDFAMVWEVDAKAQKRLKDIADALKGADRLILATDPDREGEAISWHVLEALKQ
                     RRALGKLEVQRVVFNAITKSAVLDAIAHPRAIDEELVDAYLARRALDYLVGFTLSPVL
                     WRKLPGARSAGRVQSVALRLICERELEIEAFKPQEYWTVEVDFAAPGGNFTAKLTHAD
                     GKKLDKLSLAHAEDAARAVAAIRNGTFTVAEVEKKPVKRHPWPPFITSTLQQEAARKL
                     GFAAARTMKVAQGLYEGVELGGETVGLITYMRTDGVTMAGEAIAESRQTIAELHGDRY
                     VPERPRAYSSKVKNAQEAHEAIRPTSFARHPERVSRYLDADQARLYELIWKRALASQM
                     ESAEQERTTADIASGDSETRLRATGTVTLFDGFLKLYQEGRDDEADGEDGRLPKLAGG
                     DALSTLAVRDAQHFTEPPPRYSEASLVKKLEELGIGRPSTYASILSTLRDRAYVRMDR
                     NRFVPEDKGRIVTAFLTSFFKRYVEYDFTADLEEKLDEVSAGELSWKQLLRDFWKNFH
                     TAAEDTKNLKFADVIDALDAILGPHMFPEREDGGDPRRCPTCGTGRLNLKLGKFGAFI
                     GCSNYPECRYTRQMTRGDENGEAAPRALGIDPNSGEEVALKSGRFGPYVQLGNGEKPQ
                     RASIPKGFSAETMDLEKALQLLSLPREVGVHPETGKPIMAGFGRFGPYLSHDGAYASL
                     ETPEDVFTVGINRAVTLLAERKEKGGRGPRGSQALKELGAAPGGGAPIKVMKGRYGPY
                     VTDGKVNATLPRDSDPVSVTLEEALALIAARAEKGPAKKKGRGSTAPAAKAKAPAGKK
                     KAAPKKAATETASKPAVKPKRKATK"
     gene            7104..7322
                     /locus_tag="VGM72_02140"
     CDS             7104..7322
                     /locus_tag="VGM72_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264092.1"
                     /translation="MPSTPHPQSRIDESSPKNPTPAESADYTYDLLISLKKLAVDHNQ
                     TRLARLIEAAAMEAQFLTGQGLGADDPP"
     gene            complement(7319..7939)
                     /locus_tag="VGM72_02145"
     CDS             complement(7319..7939)
                     /locus_tag="VGM72_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY4264093.1"
                     /translation="MLLVLATVFFIAGFLSLRIDRQAAHYSREQLGRPLWRFALRVTD
                     WAKGVVWIIAALLFYAGVQALMAWRGETPLLRLISGYSLALLASFVVASVVLHSIKIF
                     LGRRRPRDEFEHGLYGFRFFTWELQYDSFPSGHSMTILCVAVVLSAIVPVLAPLWLAA
                     ATALALTRAMLTAHFFSDVFVGAGIGLLIARETFAIGFPQLMPSWF"
     gene            complement(7939..9222)
                     /gene="purD"
                     /locus_tag="VGM72_02150"
     CDS             complement(7939..9222)
                     /gene="purD"
                     /locus_tag="VGM72_02150"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016659107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY4264094.1"
                     /translation="MNILLVGSGGREHALAWEISASPLLTKLYCAPGNAGIARDAECV
                     AIDAMDLAGLVAFAKEKRIDFVVIGPEGPLTAGLADRLEAAGIKALGPSVRAARLEAS
                     KGFVKDLCAEHGIPTAAYKRCGDAAKAKAFAASLSLPVVVKADGLAQGKGVAIAQTRE
                     EADAAIDAMFAGAFGDAGNEVVIEEFLEGEEASFFVLTDGSHVLPLASAQDHKRAFDG
                     DLGPNTGGMGAYSPAAVLTPEIERKVMTRIVQPTVDAMRARGTPYQGVLYAGLMIKDG
                     EPKLIEYNCRFGDPECQVLMLRLKSDLLPALIAAHDGILDSVDLRWRDDAALGVVMAA
                     QGYPGAYEKGTEIKDLKQASAVDGVEIFHAGTLANGERILANGGRVLNICASGKDVGQ
                     ARLRAYEAVDRIDWAGGFCRRDIGWRALARDEAKR"
     gene            9273..>10399
                     /gene="xseA"
                     /locus_tag="VGM72_02155"
     CDS             9273..>10399
                     /gene="xseA"
                     /locus_tag="VGM72_02155"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY4264095.1"
                     /translation="MMDETPLNRLAEPRPQGNLREFTVSELSNLLKRTVEDAFPYIRV
                     RGELSRVSRPSSGHCYFDLKDDVSVIGAVIWKTSFARLKIKPEQGIEVICTGRLTTFP
                     GQSKYQIVVEEMELAGLGALMAMLEARKKKLAAEGLFDATRKKPVPFLPRVVGVVTSP
                     TGAVIRDIVHRLSDRCPRHVLLWPVTVQGERAAGEIAAAIRGFNALSEGGAIPRPDVL
                     IVARGGGSFEDLLAFSEESVVRAAAESSIPLISAVGHETDTTLIDFASDKRAPTPTAA
                     AEMAVPVLRDCVETTLSLNQRLLRATSRGLDRQRRHLEALARALPRGDSLFALPRQRF
                     DSSAGRLSAALFQNLQRHRGQFARVSALLRPRIITAEIERGR"
BASE COUNT         1977 a         3191 c         3218 g         2013 t
ORIGIN      
        1 gattttttgt acaacgccgc gctgtggtct acagtccctc atcggatttt tgagggatga
       61 gaaatggcga agagaatggc ctcgttcggg atgttgggtg tcagctttgc ggcgctcctg
      121 gcggcaaccg cgcccgcagc atcccaagcc gtggatgccg gcgatatcgg cggcgtggtg
      181 acgggcgcca atggcccgga agccggggtg tgggttattg cggagacgcg cgaccttccg
      241 acgaaatatg tgaagatcgt cgtgaccgac gatgccggac gttaccttat ccccgatctt
      301 cccactgcca attacgacat ctgggtgcgc ggttatgggc tggtggattc gcccagggtc
      361 aaatccgcga ctggaaaaat gttgaatctg aaagcagtgc cggcgccgag cgcggctgcc
      421 gcggccgctt attaccccgc gatttattgg tattcgatgc tgaagatccc cgacccgaaa
      481 ttgtttccgg ggacgggcgt gaaaggcaac ggcatgccgg tgtcgctgaa ggatcaaggc
      541 gtctggctgc gtcagctcaa gaccgatggc tgcaattcct gccatcagct tggcgacatc
      601 gcgactcgcg agctgcccgc gcaattgggc catttcgaca gttcggcggc cgcctgggaa
      661 cgccggatcc aatcgggtca gccgggcgcg ctgatggtgc aggcgctggg cgatctcgac
      721 ggctcgcgcg cgctttctct gttcggcgat tggaccgacc gcatcaagaa gggcgagctg
      781 cctttcgcga aaccggtgcg tccggccggc atcgagcgca atgtcgtcct gacgatgtgg
      841 gattggtcgc agccgaccgc ctatatgcac gatctgacgt cgaccgataa acgcaacccg
      901 accctgaatg tgaacggcat catctacggc tcgccggaat tgagttcgga ttacgtcgcc
      961 tggctcgatc cggcgaacaa caagacgggt ctgatcaaga ccgaatggcg cgatcccaaa
     1021 acgcccagca ccaagacatc gccgatctat gcgccgtcgc cctattgggg ccaggaaccg
     1081 gtgtgggaca gccacagcct caaccacaat ccgatgatgg atgccaaggg ccgcgtgtgg
     1141 ctgacttcgc gcatccgtcc ggacgcgaat ccggctttct gcaaagccgg ttcgcatcat
     1201 ccttccgcgg aagcgtttcc gctggagacc tcgcggcgcc aattgcagat gtacgatccg
     1261 gcgaccaaga agatcaccac catcgatacc tgcttcacca cacatcatct gaatttcgac
     1321 cgcgacggaa aaatctggtt ctccagcggc agcaacaccg atcccgtggt cggctggttc
     1381 gacaccaaga aatgggacga aacccatgac gaacaggcga gccagggctg ggcgccgttc
     1441 atcgtggatg tcaacggcaa cggcaaggtg gacgcctatg tcgaacccaa tcagccgctc
     1501 gatcccaaaa aggacaagcg cgtcctgcag ggactttatt cggtgtcggc caatccgtcc
     1561 gacggcacga tctgggccgt ggtcctgggc tttccgggca ccgcgatccg cttcgatccc
     1621 aagaccaagc tgaccgaagt ctatgaagtg ccgtggaacg atccgaagaa tccgcaatcg
     1681 ggattcagcc cgcgcggcgc ggacctgacg agcgacggtg tgctgtggtc ggtgctggcg
     1741 agcggccaga tggcgagctt cgaccggcgt ttgtgcaagg gtccgctgac gggccccgtt
     1801 gcggccacgg gcaaagcctg tccggaagga tggaagctct acacgatccc cggcccgcaa
     1861 ttcaaaggcg tcgcgatgaa gggcggcagc gcggaggcgc cctattatga ttgggtcgat
     1921 cagttcgaca cgctgggcct cggcaagaac acaccgatcg cgacgggcaa tctgtcggat
     1981 gcgatgttcg ctatggtgga cggcaaatgg gtgaccatgc gcgtgcccta tccgatggga
     2041 ttctttgcca agggcatgga cgggcgtgtg gacgacgcca aggccggctg gaaaggcaag
     2101 ggcatctacg ccacctattc gggcagcgcg agcacccaca tcgaaggcgg caaagggacg
     2161 atgcccaagg tcatccactt ccagctccgg cctaatccgc tggcgaaata agtgcgttcg
     2221 caccagcggg cgcattctcc gccgccttaa ggaaaaactt tatgatcctg gggatgccga
     2281 tcgcgacgtt tacggccgtc catgtgattc taagtctgtt gggaattgcg gccggtctcg
     2341 tcgtcgtttt cggcatgatc gcgggcaaac attcaagcgc ctggacgggg attttcctcg
     2401 ccgcgacggc cctgaccagc gtaacgggat ttcccattcc tccacttggc ttcgatccgc
     2461 cgcgtgcggt gggcgtgctt tcgctgctgc tgctggcgtt cgcggtcccg gcgctctacg
     2521 tcttccgtct ggcgggatgg tggcggtgga tctatgtcgt gacggcgacc gcggccctct
     2581 acttcaactg ctttgtcggc gtcgtacaga cattccaaaa gattgctttc ttcaaaagcc
     2641 tcgcgccgac gcaatccgag ccacccttcg caatcgcgca gatcgctata ttggtcatcc
     2701 tcattgcatt aggagccttg gctgtgaaaa aatttcatcc aagaatgagc agggcgttct
     2761 gaatgagcga agaaagtttc gaaggcgccg gcggtctcag gattttcttc cggtcgtggc
     2821 gcccgactgg aatgccgcgc ggtgtggtcg tcatagttca tggcttcaac gcgcacagtg
     2881 gccaatatga ttgggtggcc gcccaattcg tggctcgcgg cttggcggtc tatgcacccg
     2941 atctgcgcgg acgcgggaat tccgatggcg agcgctatta tgtcgagaaa ttcgccgatt
     3001 atgtcggcga tgtcgctggc ctgatgacaa tcgtgaaatc gcgtgagccg cgcctgccgg
     3061 ttttcctgct tggtcattcc gccggcgggg ttgtttcctg tctgtatgcg ctcgagcatc
     3121 agagcgatct tgccgggctg atctgcgaaa gtttcgcgtt ccaggtgccc gcgccggatt
     3181 tcgttctcgc cgtgctgaaa gggataagcc atctcgcgcc gcacgcccat gtgctgcatc
     3241 tgaagaacga ggatttctca cgcgatccca aagtggtggc cgcgatgaat gccgatccgc
     3301 tgatcgctca tgagacgcag ccgacgcgca cggtcgccga aatggttcac gccgatgaac
     3361 tgctcaaaca ggattttcca ctcatcgtcc tgccggtgtt catcctccac ggcaccgccg
     3421 acaaggccac caggccgagc ggcagccagc tcttctacga caaagccggt tccaagcata
     3481 agacgctgaa attctacgag ggatatttcc acgatcccct gcacgatctc ggcaaggaaa
     3541 cggtgatggc cgatatccag gcctgggtcg ataagcggat cacgccggcg aaatagcgcg
     3601 gcgaatagct ccggcattcg ctttgattgc gggtcttggc ttaagcgccg ttcaatgcgc
     3661 ccaaaacgga gatgcccgat tgggcgtgta ggaatttgag ggtccagaat ttgggtaaat
     3721 ctgcgagttg ggaaagccct gcgaattggg aaagcccaac ggtaagtcct gggaattggg
     3781 gaagccctga cctgagaatc ctcgatcggg aaagcctgaa ttataggagc ctcggtcgta
     3841 aaagccctga ttggcgaaac caggaccgat gccccgggac gtcgtgacgc caaaggagaa
     3901 tgagctgctt ccaaacgtgg tggtcgtttg gcccgggccc gaggtgaatg tctggggcct
     3961 ggaagccgga tcggaaaagc gcggcgtccc gtcgctgttg gagggagaag gaaccgtcga
     4021 aatcgcccaa cacggaccgg caacaaaaag cgcggccgcc aacacgggcg cgctaacaaa
     4081 cgaaaatcca cgagacatgg gaaccacctc tttgccgcgc actcttcata gggcgcttcg
     4141 aaagcgcgcc tagcaactaa aataagcgca atcgcaggtt cttcacggaa aatatggcca
     4201 tcggctttcg taattttcca gccatcgtca tattcccgct tggggttatt ttgtcgcttt
     4261 gcgcttgggc ttgacggccg gtttggacgc cgtctcggtg gccgcttttt tcggggccgc
     4321 ctttttcttt ccggctggtg cttttgcctt ggcggcaggg gcggtcgacc cacggccctt
     4381 tttcttggcc ggacccttct cggcgcgcgc ggcgatcagg gcgagggctt cttccaatgt
     4441 caccgatacg ggatcggaat cgcgcggcag cgtcgcgttc accttgccgt ccgtgacata
     4501 gggcccgtaa cggcctttca tcaccttgat cggcgcaccg cctcccggcg ctgctcccaa
     4561 ttccttcagc gcctgactgc cacgcgggcc gcgcccgccc ttttccttgc gttcggcgag
     4621 cagtgtcacc gcgcggttga tgccgacggt gaagacatcc tccggcgttt ccagcgaggc
     4681 ataggctccg tcatgcgaga gataggggcc gaagcgcccg aagcccgcca tgatgggttt
     4741 gccggtttcg ggatgaacgc ccacttcgcg cggcagcgac aaaagctgca acgccttttc
     4801 caaatccatc gtctcggcgg agaagccttt gggaatgctg gcgcgctggg gcttctcgcc
     4861 attgcccaat tgcacatagg ggccgaaacg ccccgatttc aacgcgactt cctcgcccga
     4921 attcggatcg atgccgagcg cgcgcggcgc cgcctcgcca ttctcatcgc cccgcgtcat
     4981 ctgccgcgtg tagcggcatt cgggataatt cgagcagccg atgaacgcgc cgaattttcc
     5041 gagtttcaaa ttcagccgcc cggtgccgca ggtggggcag cggcgcgggt cgccgccatc
     5101 ttcgcgttcc ggaaacatat gcggtccgag gatcgcatcg agcgcatcga tgacgtcggc
     5161 gaatttcagg ttcttggtgt cttcggccgc ggtgtggaaa ttcttccaga aatcgcgcag
     5221 caattgtttc cacgaaagtt cgcccgcgga aacctcgtcg agcttctctt ccagatcggc
     5281 ggtgaaatca tattcgacat agcgtttgaa gaagctggtc aggaacgcgg tgacgatgcg
     5341 ccccttgtcc tcgggcacga aacgattgcg gtccatgcgc acataggcgc ggtcgcgcaa
     5401 ggtggaaagg atgctggcat aggtcgaggg ccggccgatc ccgagttcct ccagcttctt
     5461 gaccaggctt gcctcggaat agcgcggcgg cggttcggtg aaatgctgcg cgtcgcgaac
     5521 cgcgagcgta gaaagtgcgt cgccacccgc aagcttcggc aggcgcccgt cctcgccatc
     5581 ggcctcatcg tcacgaccct cctgataaag tttcaggaag ccgtcgaaca aagtcaccgt
     5641 gccggtggcg cgaagccgcg tctcgctatc gcccgaagcg atatcggcgg tggtgcgctc
     5701 ctgttcggcc gattccatct ggctcgcgag cgcgcgcttc cagatcagct catagagccg
     5761 cgcctgatcg gcatcgagat agcgcgagac acgttcgggg tgccgcgcga agctggtggg
     5821 gcggatcgcc tcatgggctt cctgcgcgtt cttcaccttg gaagaatagg cgcgcgggcg
     5881 ttcggggaca taacgatcgc catgcagttc ggcaatggtc tgacggcttt ccgcgatggc
     5941 ttcgcctgcc atggtgacgc catcggtccg catataggtg atcaggccca cggtctcgcc
     6001 gcccagctcg acaccttcat acaggccttg tgcgaccttc atcgtgcgcg ccgcggcgaa
     6061 acccaatttg cgcgcggctt cctgttgcag agtcgaggta atgaagggtg gccagggatg
     6121 acgtttgacc ggctttttct cgacctcggc gaccgtgaat gtgccgtttc ggatggcggc
     6181 gaccgcgcgt gcggcgtcct cggcgtgggc gagcgagagt ttgtcgagct ttttgccgtc
     6241 cgcatgcgtc agcttggcgg tgaaattgcc gccgggcgcc gcgaaatcga cttcgacggt
     6301 ccaatattcc tggggcttga acgcctcgat ctcaagctcg cgctcacaga tcagcctgag
     6361 cgccaccgat tggacgcggc ctgccgagcg cgcccccggc agctttcgcc agaggaccgg
     6421 agaaagcgtg aacccgacga gataatcaag cgcccggcgc gcgagatagg catccaccag
     6481 ctcttcatcg atggcgcgcg gatgggcgat ggcgtcaagc acggccgatt tggtgatggc
     6541 attgaatacg acgcgctgga cttccagctt gccgagcgcg cgccgctgct tcagagcttc
     6601 cagcacgtgc caggagatcg cctcgccttc acggtctggg tcggtggcga ggatgaggcg
     6661 gtccgcgccc ttcaaggcat cggcgatatc tttcagccgt ttttgcgcct tggcatcgac
     6721 ttcccagacc atggcgaaat cggaattggg atcgaccgag ccgtccttcg cgggaagatc
     6781 ccgcacatgg ccgtagctcg ccaaaaccgt gtaatcccgg cccagatatt tgttgatggt
     6841 cttcgctttg gcaggcgatt ccacgaccac gacgctggaa ggttctgaca tcggctccgg
     6901 ctggtgagac tgtcacaatt gcgctttcag gccttggctg cggctgttcg gcgcggaggg
     6961 cgcggacact ggggtacgga ccggatgtgt gtcaacgtgt taatcccgca agttttagta
     7021 acgcataata ttatcacaac tatagattgc aaacttcggc taccgtccgt tatggtacgg
     7081 aatcagccaa acaaaagatt cccatgccaa gcacacccca tccacagtcc cgcatcgacg
     7141 aaagttcccc taagaaccct accccggccg aaagcgcgga ttatacgtac gatctattga
     7201 tttctttgaa gaaattggcg gtggaccaca accagaccag gctcgctcgc ctgatcgagg
     7261 ctgcggccat ggaagcccaa tttctgaccg gccaagggct tggcgcggac gatccaccct
     7321 agaaccagga tggcatcagt tgtggaaagc cgatcgcaaa ggtctcccgc gcgatcagga
     7381 gccctatgcc ggcgcccacg aacacgtccg agaaaaaatg tgcggtcagc atggcccgcg
     7441 tcagtgcgag ggcggtcgcg gctgccagcc ataagggcgc gagcacggga acaatcgcgg
     7501 acagcaccac cgcgacgcag aggatcgtca tggaatggcc cgaggggaag gaatcatatt
     7561 gcagctccca ggtgaaaaac cggaatccgt aaagcccatg ctcgaactcg tccctgggcc
     7621 gtctgcgtcc gaggaaaatc ttgatgctgt gcagcaccac gctcgccacg acaaagctcg
     7681 ccagcagcgc cagcgaatag cccgagatca gacgcagcaa tggcgtttcg ccgcgccacg
     7741 ccatgagcgc ctgcacgccg gcatagaaca gcagggcggc gatgatccag acgaccccct
     7801 tcgcccaatc ggtaacgcgg agcgcaaagc gccacagcgg acggcccagt tgctcgcgcg
     7861 aataatgcgc ggcctggcgg tcgatgcgca gtgagagaaa tcccgcgatg aaaaagacag
     7921 tcgcgagcac aagcagcatt agcgcttcgc ctcgtcgcgc gcgagcgccc gccagccgat
     7981 gtcgcgccgg caaaacccgc cagcccaatc gatgcggtcg acggcctcat aggcgcgcag
     8041 ccgggcttgg cccacatcct tgcccgaggc gcagatgttc agaacccgcc cgccattggc
     8101 caggatgcgt tcgccatttg cgagcgttcc ggcatggaag atttccacgc cgtccacggc
     8161 ggacgcctgt ttgaggtctt tgatctcggt gcccttctca taagcgccgg ggtagccttg
     8221 cgccgccatc acgacgccga gcgcggcgtc gtcgcgccaa cgcagatcga cgctgtcgag
     8281 aatgccgtcg tgcgccgcga tcaaggccgg cagcagatcg gatttcagcc gcagcatgag
     8341 cacctggcat tccggatcgc cgaagcggca attatattcg atcagcttcg gctcgccgtc
     8401 cttgatcatc agtccggcat agagcacgcc ctgatacggt gttccgcgtg cacgcatggc
     8461 atcgactgtc ggctgaacga tgcgcgtcat caccttgcgc tcgatttcgg gagtgaggac
     8521 agcagcggga gaataggcgc ccatcccgcc ggtgttgggc cccaaatcgc catcgaaagc
     8581 gcgcttatga tcctgggcgc tcgcgagcgg cagcacatgc gagccatccg tgagcacgaa
     8641 gaagctggct tcttcgcctt ccaggaattc ctcgatcacg acttcattgc cggcgtcgcc
     8701 gaaggcgccc gcgaacatcg catcgatggc ggcatcggcc tcttcgcgcg tttgcgcgat
     8761 cgccacgcct tttccttgcg cgagtccatc ggctttcacc acgaccggta agctcagcga
     8821 cgccgcaaag gccttggcct tggccgcatc gccgcagcgt ttgtacgccg ccgtcggaat
     8881 gccgtgctcc gcgcacagat ccttgacgaa gcctttggag gcttccagcc gcgcagccct
     8941 gaccgatggt ccgagtgcct tgattcccgc agcctcgaga cgatccgcca atcccgccgt
     9001 cagcggacct tccgggccga tcaccacaaa gtcgattcgc ttttctttcg cgaacgccac
     9061 gagtccggcc aaatccatcg cgtcgatggc gacgcattcc gcatcgcgcg cgattcccgc
     9121 attgcccggc gcgcaataaa gcttggtcaa cagcggactt gccgaaattt cccaggcaag
     9181 cgcatgctcg cgtccgcccg atccgacaag aagaatgttc aagggggttt ccctgctggc
     9241 gtgctcaagg accagggctg tatcattggc gcatgatgga cgaaacccct ttgaaccgtt
     9301 tggccgaacc gcgcccacag ggaaatctgc gggaattcac ggtcagcgaa ttgtccaacc
     9361 ttctgaagcg caccgtggaa gatgcctttc cctatatccg ggtgcgaggc gagctgtcgc
     9421 gggtgtcgcg cccttcgagc ggccattgct atttcgacct caaggacgac gtctcggtga
     9481 tcggcgccgt catctggaag accagctttg cccggctgaa gatcaagccc gaacagggaa
     9541 tcgaggtgat ctgcaccggg cggctcacca catttcccgg ccagtcgaaa tatcagatcg
     9601 tcgtcgagga gatggaattg gccggcctcg gcgcgctgat ggcgatgctg gaggcgcgaa
     9661 agaaaaagct cgcggccgaa ggcctgttcg atgccacgcg caagaagccg gtgccgttcc
     9721 tgccgcgtgt cgtgggcgtg gtgacttcgc cgaccggggc tgtgatccgc gatatcgtgc
     9781 accggttgtc ggaccggtgt ccgcgtcacg tcctgctgtg gccggtgacc gtgcagggcg
     9841 agcgcgcggc cggcgagatc gccgcggcga tccgcggttt caatgcgctg tccgagggcg
     9901 gagcgatccc gcgtcccgac gtgctgatcg tcgcgcgagg cggcggctcg ttcgaggacc
     9961 tgctcgcatt cagcgaggaa tcggtcgtgc gtgcggcggc ggaatcgtcc attcccctga
    10021 tctcggcggt ggggcatgag accgatacga cgctgatcga tttcgcgtcc gacaagcgcg
    10081 cgccgacgcc cacggcggct gccgagatgg cggtgccggt gctgcgcgat tgcgtggaaa
    10141 cgacgctttc gctgaatcag cggctgttgc gggccacatc gcgcgggctc gaccggcagc
    10201 gccgccacct ggaagcgctc gcgcgggcgc tgccacgcgg cgactcgctt tttgcgctgc
    10261 cgcgtcagcg tttcgattcg agcgcggggc gcctcagcgc cgcgctgttc cagaatctgc
    10321 aacgtcaccg ggggcaattc gcgcgggtct ccgcgctgct gcgtccgcgc atcataactg
    10381 ccgaaatcga gcgcggccg
//
LOCUS       DASYEG010000056         8282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3786842, whole genome shotgun sequence.
ACCESSION   DASYEG010000056 DASYEG010000000
VERSION     DASYEG010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 8282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8282
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3786842"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..1565
                     /locus_tag="VGM72_02160"
     CDS             219..1565
                     /locus_tag="VGM72_02160"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4264096.1"
                     /translation="MSVSASLPSRSRFDGWRLYFRPRVATMLMLGFSSGLPFMLTANT
                     LSYWLRDAGISLTAIGFLSWVGLAYSLKPLWAPLVDRLDAPIFGRLGRRRGWMFAAQI
                     LVCLGLLGMSLLGPGGGLAAIGICALVVAFASSTQDIVVDAWRIEIAEDGNELGLLSS
                     AYTLGYRAALLVSDAVILILANHLSWQIAYGVMAALMGVGFFATLKAAEPVRADRVME
                     ANERERPLFSLRGFFDAVVGPFIAFFRTHQWLGLLILAVISLYQIPNFVSGPMSSTFY
                     SDVGLSKDAVGTIRGSVGLISSLVGIAAAGISAARFGYLPTLVAGIALEAIAVAAFIP
                     LAYSGADLRVFTAMMAVDSFGLAFAGVALVTYMSSLTSLGYTATQYALLSSTYTWLGK
                     ILKGFSGQTVDALAHGRNLMDAYAIFFAGAAAIGLPALVLCLFLAQARNTVPATTP"
     gene            1746..2714
                     /locus_tag="VGM72_02165"
     CDS             1746..2714
                     /locus_tag="VGM72_02165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HEY4264097.1"
                     /translation="MGPARRVKFRFGIGRRRWGFARRPQFGRRVAQAMGFQQETAPAL
                     ISVHEQMPPAHREHAWPVLAAHGSLEVRIAESEYEIEASQRLRYRVFYEEMSAIPTPQ
                     MREQQRDFDRYDEFCDHMLVVDREVRDAQGEPAVVGTYRLLRGEVAARHGGYYTSSEY
                     DLTPMLKGNPPGTRYLELGRSCVLKNYRAKATTMQLLWRGIVVYLDRYAIDVMFGCGS
                     LPGIDPDALKLPLSYLHHFHSTPEQQRVAARHELYVSMNRMAKADIDPKEALRKLPPL
                     VKGYVRAGSYIGDGAVIDKQFGTTDVLIYFPVAHIDQRWRTKFGRG"
     gene            complement(2721..3695)
                     /locus_tag="VGM72_02170"
     CDS             complement(2721..3695)
                     /locus_tag="VGM72_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY4264098.1"
                     /translation="MSDAEPFTAPEVRIPESAARAPAARGHDLGGYLLAATGAILFSM
                     KAVIVKLAYGLHVDPETLLALRMGFSLPFYLGIGFLAVRGRRHKLAAIPSLNRTIRAA
                     LIGMLGMWVASYTDFLGLQYISAQFERLILLTYPLFVVLFGAMFFGQTVRPRALLALG
                     VSYAGLALIFGESVSLEGHDVAIGAGWVLVAAISFGLYQLLAKNAIDEMGPKLFTCIA
                     MTGASIAAFIQFFAHHSGAELLVSGPLLFYSVLVAIAATVLPSFFMSAALHRISAQAN
                     STIGVLSPVSTIILAAIILGERMNWMSVFGTLLVIGGVGWFTLADRRS"
     gene            complement(3692..4609)
                     /locus_tag="VGM72_02175"
     CDS             complement(3692..4609)
                     /locus_tag="VGM72_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEY4264099.1"
                     /translation="MVQSVPELTRAWLASLAHERRLSPHTLRAYGDDAERFLGFFAFH
                     RGAAVDLSALQGLRPADVRAFLTARRAEGLGARGVQRALAALRSFYRYLERENLAGSA
                     ALRSVKSPKLPRTLPRPLSEIDAARTIDEAGQDEEPWIAARNAALITLLYGTGLRISE
                     ALSLKRGDTPLGETLTILGKGSKERAVPIIAAVRDAVDHYAQACPFERERAGALFLSR
                     RGKPMSPREAQGLMQNLRGRLGLPSSATPHALRHSFATHLLTNGGDLRAVQELLGHAS
                     LSTTQKYTEVEGARVLAVYEAAHPRARAK"
     gene            4688..6883
                     /locus_tag="VGM72_02180"
     CDS             4688..6883
                     /locus_tag="VGM72_02180"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEY4264100.1"
                     /translation="MQAQAGIALAPAVETRAAVLLPLPLPAAYDYILPEGVVPKRGLI
                     VRAPLGGRTLIGIVWGKAEGGVARDKLRAAEPIAEFRLPPMLCDFIDWVARYTLSPPG
                     AVLAQALRVKSAFDPEIPRRALRIGAIRPARLNPARARVLSLMADGLARTPPQIAELA
                     AVSAGVVRSLSESGALIGVNLPEFAPCPEPDAAFDAVTLNPDQLRAAEHLRDTVKKRE
                     FSVALLDGVTGSGKTEAYFEAVAEALSEGKQALILLPEIALTVQFLDRFEARFGCRPV
                     EWHSDLSLRDRKRAYRAVMHGEARVVVGARSALFLPFTALGLIIVDEEHEHAYKQEEG
                     VIYHARDMAVVRARLENCAIVLASATPSLETYVNARTGRYEWLKLAHRHGAAEMPEVK
                     LVDLREQQGEPGQFLSPVLRAALDGTLRAGEQALLFLNRRGYAPLTLCTQCGHKQTCR
                     NCSAWMVEHRYRKRLVCHHCAYETAIPQHCPQCAAENSIIACGPGVERVEEEFRAAFP
                     EARVAIASSDTLLGPAQTQATIRAFAKGEIDVIIGTQIVAKGHHFPQLTLAGIIDADL
                     GGGAGDPRAAERSFQLLHQVAGRSGRGEKPGLVLIQTRNPDDPVMRALASQARDDFLE
                     EQMRERERGGAPPFGRLASLILAGADAVAVRETGRALASAAPRTRGLTVWGPAPAFYQ
                     MLRGRTRERLLVQAEKNIDIQGYIRAWLAGVKIPGSVRLTVDIDPISFF"
     gene            7077..>8282
                     /locus_tag="VGM72_02185"
     CDS             7077..>8282
                     /locus_tag="VGM72_02185"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEY4264101.1"
                     /translation="MTESTNSDAGLSSSARITRARVVIWVLCLLIILAAGIVPQGFFE
                     ISSNSVFDAYQQFSPSRVLPSPTILVDVDDESLRRVGQWPWRRDQIAQMIDAAAGARV
                     VGLDILLTEQDRLSPKALLSDLPNIPPEIVQALSALPQSDAVLAKSLSRVPVVLAAAA
                     RTAGDDPSRAPVAMAPIFETGVDPRPAMPRYRSVAWPLPELVAASRGIGLVSEVSESD
                     GVTRRMSVLLAIGPVLMPSFAAEVVRVASNAPRIVLLSGPAGGRELQIGDHRVALDTA
                     GRVWPHLGKRPEGESVPAYRLIDGSIPPSFFRDRIVLMGVGASGLGNVSVTPLRQTEP
                     PMAIQAHLIDSLMSGDVLWRPPMVKSMEVLFALALAAAALLLMGRISDLAYGILFGAI
                     ALVSIIGSFV"
BASE COUNT         1399 a         2588 c         2670 g         1625 t
ORIGIN      
        1 atccggaccc gctcaacccg atatcctcga cctcggccgc gggacctatc ctcgaatcga
       61 gctgcgcgga gaatcccaat tcgctgttcg gcgccgaggg cgcgttgccg atcccgaccg
      121 cgaacagctc cgatttctga gggttgcggc cggcacgtcg aatgcgcctt ttggcgcgtt
      181 ctcgtctatg attcgcgaac gtgttgcaga gtagcccgat gagcgtttcg gccagtcttc
      241 catctcgcag ccgtttcgat ggctggcggc tctatttccg cccgcgcgtg gcgaccatgc
      301 tgatgctggg cttttcctcc ggtctgccgt tcatgctcac cgccaacacg ctgtcctatt
      361 ggctgcgcga tgccggcatc tcgctcacgg cgatcggatt tctgtcctgg gtcgggttgg
      421 cctattccct gaagccatta tgggcgccgc tggtcgaccg gctggatgca ccgattttcg
      481 ggcgtctcgg caggcggcgc gggtggatgt tcgccgctca gatccttgtg tgtctcggtc
      541 ttctcggcat gtcgctgctt ggcccgggcg gtggtttggc ggcaatcggg atctgtgcgc
      601 tggtcgtggc gttcgcgtcc tcgacacagg acatcgtggt ggatgcctgg cgcattgaaa
      661 tcgccgagga cggcaatgag cttggcctgc tgtcgtcggc ctatacgctg ggttaccgcg
      721 ccgcgctcct ggtgagcgat gcggtcattc tgattctggc caatcatctg agctggcaga
      781 tcgcctatgg cgtcatggcg gccctgatgg gggtcggatt tttcgccact ttgaaggccg
      841 ccgagcccgt acgcgcggac agggtgatgg aggcgaacga acgcgaacgt ccgcttttca
      901 gtctgcgcgg ctttttcgat gcggtggtgg gccctttcat cgccttcttc cggacgcacc
      961 aatggctggg acttctgatt ctggcggtga tcagcctcta tcaaattccc aatttcgtct
     1021 caggcccgat gtcgagcacc ttttacagcg atgtcgggct ttcaaaggat gcggtgggca
     1081 ctatccgcgg ctcggtcggg ctcatttcgt ctctggtcgg cattgccgcc gccggcatca
     1141 gcgccgcgcg tttcggctat ttgccgaccc tggttgcagg gatcgcgttg gaagcgattg
     1201 cggtggccgc ctttattccg ctggcctatt cgggtgccga tctgcgtgtc ttcaccgcca
     1261 tgatggcggt cgacagtttt gggcttgcct tcgcgggcgt ggcactcgtg acctatatgt
     1321 cgagcctgac cagccttggc tatacggcga cgcaatatgc gctcctttcc tcgacctata
     1381 cgtggctcgg gaaaatcctg aagggcttct ccggtcagac cgtggatgcg ctcgcgcacg
     1441 ggcgcaacct gatggatgcc tatgcgatat ttttcgcggg cgcggcagcc atcggtctgc
     1501 cggcgttggt cctgtgcctg tttcttgcgc aggcgcgaaa tacggtcccg gccaccaccc
     1561 cttagcgggg cgacctcgcg aagcttttat gaattccctc tgaaagtcac tgggcgcggt
     1621 ttccggcggc gtggtagaat gtcatgattg ttggtgcggt aggggtaacc aaaccggaac
     1681 gggtccggca tttcccgtag catcccgcta ggtcttggct ttccccttcg gcgctggaga
     1741 acgatatggg tcccgcgcgt cgtgtgaaat ttcgtttcgg catcggccgg cgccggtggg
     1801 gattcgcgcg ccggccgcaa ttcggccgcc gcgtggcgca ggcaatggga tttcagcagg
     1861 aaaccgcacc ggcgctgatt tccgttcatg agcagatgcc gccggcccac cgtgaacacg
     1921 cttggccggt gctggccgcg catggatcgc tcgaagtgcg tattgccgaa tccgaatatg
     1981 agatcgaggc ctcgcagcgc ctgcgctatc gcgtctttta tgaggagatg tcggcgatcc
     2041 ccacgccgca gatgcgcgaa cagcagcgcg atttcgatcg ttacgacgaa ttctgcgacc
     2101 acatgctggt ggtggaccgc gaggttcgcg atgcgcaggg cgaacccgcc gtggtcggaa
     2161 cctatcgcct gctgcgcggt gaagtcgcgg cgcgacatgg cggctactac acttcgtcgg
     2221 aatatgattt gacgccgatg ctcaagggca acccgcccgg aacgcgttat cttgagctgg
     2281 gacgttcctg cgtgctcaag aattatcgcg ccaaggcgac gacgatgcag ctcttgtggc
     2341 gtggcatcgt ggtctatctc gaccgctatg ccatcgatgt gatgttcggc tgcggctcgc
     2401 ttcccggcat cgatcccgat gcgttgaagt tgccgctgtc ctatctccat catttccaca
     2461 gcacaccgga acaacagcgc gtggccgcgc ggcacgagct ttacgtctcc atgaatcgca
     2521 tggccaaggc cgacatcgac cccaaggaag cgctgcgcaa gctgccgccg ctggtgaagg
     2581 gctatgtgcg cgcgggctcg tatatcggcg atggcgccgt catcgacaag caattcggca
     2641 ccacagacgt gctgatctat ttcccggtcg cccatatcga tcagcgttgg cgcaccaaat
     2701 tcggccgcgg ctagagcagc ctagctgcgc cggtcggcga gcgtgaacca gcccacgcct
     2761 ccgatcacca gcagcgttcc gaaaacgctc atccaattca tgcgttcgcc gaggatgatc
     2821 gcggcaagga tgatggtcga caccgggctg agcacgccga tggtcgaatt ggcctgcgcc
     2881 gaaatgcgat gcagcgccgc gctcatgaag aagctcggca acaccgtggc ggcaattgcg
     2941 accagcaccg aatagaagag aagcggtccg ctgaccagaa gctccgcacc gctgtgatgc
     3001 gcgaagaatt ggatgaaggc cgcaatgctg gcccccgtca tggcgataca ggtgaaaagt
     3061 ttcggaccca tctcgtcgat ggcatttttg gcgagcaatt gatacaggcc gaaggaaatc
     3121 gcggcgacga gcacccagcc cgctccgatc gcgacatcat gaccttcaag cgaaacactc
     3181 tcgccgaaga tgagcgcgag tcccgcatag gaaacaccga gtgccaagag cgcgcgcgga
     3241 cgcaccgtct ggccgaagaa catcgcgccg aacaggacca cgaatagcgg ataggtgagc
     3301 aggatcagcc gctcgaattg tgccgagata tattgcaggc cgaggaaatc ggtgtagctc
     3361 gcgacccaca tgccgagcat gccgatgagg gccgcgcgga tggtgcggtt cagcgaggga
     3421 atcgcggcga gcttatgccg cctgccgcgc accgccagaa atccgatacc gagataaaag
     3481 gggagcgaaa atcccatccg caaggccagc agcgtctcgg gatcgacatg aagcccgtaa
     3541 gcgagcttga cgatgacggc cttcatcgaa aacaggatcg cgccggtggc cgccagcaga
     3601 tagccgccca gatcgtgccc gcgcgccgcc ggtgcccggg cggcagattc gggaatgcgc
     3661 acttcgggcg cggtgaacgg ctcggcgtcg ctcatttcgc ccgcgcacgg ggatgggccg
     3721 cttcgtagac cgcgagcacg cgcgcgcctt cgacttcggt gtatttctgt gtcgtggaca
     3781 gcgaggcgtg gcccagcaac tcctgtacgg cgcgcagatc gccgccattg gtcagcagat
     3841 gcgtggcgaa ggaatggcgc agcgcatggg gtgtcgcgct cgatggcaga ccgagccggc
     3901 cgcgcagatt ttgcatcaag ccttgcgcct cgcgcgggct catgggtttg ccgcgccgtg
     3961 aaagaaacag cgcacccgcg cgctcccttt cgaaggggca ggcctgcgca taatgatcga
     4021 ccgcgtcgcg caccgcggca atgatcggga cggcgcgctc cttgctgccc ttgccgagga
     4081 tggtcagtgt ctcgccgagc ggcgtgtcgc cgcgtttcag gctgagggct tcggagatgc
     4141 gcagacccgt cccgtaaagc agcgtgatca gcgcggcatt gcgcgcggcg atccatggct
     4201 cctcatcctg tcccgcttcg tcgatggtgc gggcggcatc gatttcgctc aaagggcgcg
     4261 gcagggtccg cgggagcttc ggactcttga cgctgcgcaa ggccgcgctg ccggcaagat
     4321 tttcgcgttc gagatagcga tagaacgaac gcagcgccgc cagcgcccgc tgcacgccgc
     4381 gcgcgcccaa tccttccgcg cgccgggcgg tcagaaaggc gcgcacatcc gcaggccgca
     4441 agccctgcag cgcggacaaa tccaccgcag ccccgcgatg aaaggcgaag aagccgagga
     4501 agcgctcggc gtcgtcgcca taggcgcgca gcgtgtgggg gctcaagcgc cgttcatggg
     4561 ccaggctcgc cagccacgcg cgcgtgagtt ccggaacgct ttgtaccatg gacgtggcat
     4621 atcctgcttg accgggcggg ccgacgcgcg aaactaaccc gcaaaatcaa gggatgaagg
     4681 aaaacgcgtg caggctcaag ctggaatcgc gctcgcaccc gcagtggaaa cccgcgccgc
     4741 cgtgctgctg ccgctgcctt tgcccgcagc ctacgactat atcctgcccg aaggcgtggt
     4801 gccgaaacgt ggacttatcg tgcgcgcgcc tttgggcgga cgcacgctga tcggcatcgt
     4861 ctggggaaag gccgaaggcg gtgtcgcgcg cgacaaattg cgggccgccg agccgatcgc
     4921 ggagtttcgc ctgccgccca tgctttgcga tttcatcgat tgggtggcgc gctacacgct
     4981 ctcacccccc ggagccgtgc tggctcaggc gttgcgcgtg aaaagcgcgt tcgatccgga
     5041 aatcccgcga agagcgctca ggataggagc catcagacct gcgcggctca atccggcgcg
     5101 cgcgcgcgtg ctgtcgctga tggcggacgg tttggcgcgc acgcctcctc agatcgccga
     5161 gctggccgct gtgtcggcgg gcgtggtgcg cagtctctcc gaatcgggcg cgctgatagg
     5221 ggtgaacctg ccggagttcg cgccttgccc cgaacccgac gcggccttcg atgccgtcac
     5281 gctcaacccc gatcaactgc gcgccgccga gcatctgcgc gacacggtga agaagcgcga
     5341 attctccgtg gcgctgctcg acggcgtcac cggttcgggc aagaccgagg cctatttcga
     5401 agcggtggcc gaggcactga gcgaaggaaa gcaggcgttg atcctgctcc ccgaaatcgc
     5461 tctgacggtc cagttcctcg accggttcga agcgcgcttc ggttgccggc cggtggaatg
     5521 gcattccgat ctttccttgc gcgaccgcaa gcgcgcctat cgcgcggtga tgcatggcga
     5581 ggcgcgcgtg gtggtgggcg cgcgctcggc gctgttcctg ccgtttaccg cgctcggcct
     5641 catcattgtc gatgaagagc atgagcacgc ctacaaacag gaagaaggcg tgatctatca
     5701 cgcacgcgac atggcggtgg tgcgcgcgcg tctggagaat tgcgcgatcg tgctggcctc
     5761 ggccacgccg tcgctcgaaa cctatgtcaa cgccaggacc ggccgctatg aatggctgaa
     5821 actcgcccac cgccatggcg ccgccgaaat gcctgaggtg aagcttgtcg atcttcgcga
     5881 acagcagggt gaaccggggc aattcctctc gccggtgttg cgggccgcgc tggacggcac
     5941 gttgcgcgcc ggcgagcagg cactgctgtt cctcaatcgt cgtggttatg cgcctttgac
     6001 cctgtgcacc caatgcggcc acaagcagac ctgccgcaac tgctcggcct ggatggtgga
     6061 gcatcgctat cgcaagcggc tcgtgtgcca tcattgcgcc tacgagaccg cgattcccca
     6121 acactgcccg caatgcgccg cggaaaactc catcatcgcc tgcgggccgg gcgtggagcg
     6181 cgtggaggaa gaattccgcg ccgcctttcc ggaggcgcgg gtggcgattg cctcgagcga
     6241 cacgctgctc ggccccgcac aaacccaggc cacgatccgc gctttcgcga aaggggagat
     6301 cgacgtcatc atcggcacgc agatcgtcgc caagggtcat cattttccgc aactgacgct
     6361 ggccggaatc atcgacgcgg atttgggcgg gggcgcaggc gatccgcgcg ccgccgagcg
     6421 cagcttccaa ttgctgcatc aggtggcggg ccgttctggc cgtggcgaaa agcccggcct
     6481 cgtgctcatc cagacgcgca atccggacga tcccgtgatg cgggcgctgg cctcccaggc
     6541 gcgcgacgat ttcctggaag agcagatgcg tgagcgtgag cgcggcggcg caccaccctt
     6601 tgggcgtctc gcctcgctga tccttgcggg cgccgacgcc gtggcagtgc gcgagacggg
     6661 ccgcgcattg gcgtcggcgg cgccgagaac gcgcggtctg accgtatggg ggccggcgcc
     6721 ggcgttctat cagatgctgc gtgggcgcac ccgcgaacgt cttctggtgc aggccgaaaa
     6781 aaatatcgac atccagggct atatccgcgc ttggctcgcg ggcgtgaaga ttccggggag
     6841 cgtccggctc actgtcgaca tcgatcccat cagctttttc taaggatcaa atattgccat
     6901 tccatgccgg gcggcaaccg cgtcgaagtc gtcagccgcg ataacgacgc ggctattggg
     6961 ctcgattgtt aaggtttgca ggaaatcgtc cgtatcccga gaatatcggc ccggatggta
     7021 taggccaaag gcgatttcgt atcttaaact cggcagtgcc gttgccgggt ggtgccttga
     7081 ccgaatcgac gaacagcgac gccggattgt ccagctcggc ccgcataacg cgggctcgtg
     7141 tcgtgatctg ggttctttgt ttgctgatca tcttggctgc ggggatcgtt ccgcagggtt
     7201 tttttgaaat atcctccaat tcggttttcg acgcctatca acaattctcg ccctcgcgtg
     7261 tattgccctc ccccaccatc cttgtcgacg tggacgacga gtcgctccgg cgggtcggcc
     7321 aatggccttg gcgacgcgac cagatcgctc aaatgatcga cgctgcggcc ggtgcgcggg
     7381 tcgtggggct cgatatcctg ctgacggaac aagatcgcct gtcgcctaaa gcattgctct
     7441 cggatttgcc gaatatccca ccggagatcg ttcaggccct atccgcactg ccccaatccg
     7501 acgcggtatt ggcaaaaagc ttgagccggg ttccggtggt ccttgccgca gcggcacgga
     7561 cggccggcga tgatccgtct cgcgcgcctg tcgccatggc gccgatcttc gagacgggcg
     7621 tcgacccaag gcctgccatg ccccgttatc gctcggtggc ttggccattg ccggaactgg
     7681 tggcggcctc acgcgggatc ggactcgtct ccgaggtgtc agaatccgat ggcgtcacgc
     7741 gccggatgtc cgtccttctc gcgatcggtc ccgtgctcat gccgagcttt gcagcggagg
     7801 tcgtccgggt cgcgtccaat gctccgagga tcgtactgct atcgggtccg gcaggcggcc
     7861 gcgaactcca gatcggcgac catcgcgtgg ccctcgacac ggcgggacgc gtctggccgc
     7921 atctcggcaa acgtccggaa ggcgaatccg tcccggccta tcgtttgatc gacggttcga
     7981 tcccgccgtc attttttcgc gatcgaatcg tcttgatggg cgtgggtgcc tcgggactcg
     8041 gcaatgtcag cgtgacaccg ctgcgccaaa ccgaaccacc catggccatc caggcgcatc
     8101 ttatcgacag tttgatgtca ggcgatgtgt tgtggcggcc accgatggtc aagtccatgg
     8161 aggttttgtt tgccttggcg ctggccgccg ctgccttgct gctgatgggc cgcatatcgg
     8221 atttggcgta tggcattctg tttggcgcca tcgcgctcgt ttcgatcatc ggctcttttg
     8281 tt
//
LOCUS       DASYEG010000057         3076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2772294, whole genome shotgun sequence.
ACCESSION   DASYEG010000057 DASYEG010000000
VERSION     DASYEG010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2772294"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..1097)
                     /locus_tag="VGM72_02190"
     CDS             complement(198..1097)
                     /locus_tag="VGM72_02190"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase family protein"
                     /protein_id="HEY4264102.1"
                     /translation="MCGIVGLFLKNPALQSQLGKHVEIMLVGMSERGPDSAGIAVYHS
                     EVPEGQSKITLFNADVHYPWRGVADGLAKALKVKTEIEQKGNHALLHAHCSEDKVLPW
                     LRANHPDVRIMGYGKLMEVYKDMGLPAEVAARYGLAGMEGSHAIGHTRMATESAVTTE
                     HSHPFTPASDMCLVHNGSLSNHNNLRKWLRHQGQEFETDNDSEVAARYFAYRLSEGAT
                     LEEALEAGLVDLDGFFTFTMGTKDGFAVVRDGIACKPAVLAENDDWVAMASEFRSLAY
                     LPNIEKAKIWEPAPATVYTWSLH"
     gene            complement(1216..1902)
                     /locus_tag="VGM72_02195"
     CDS             complement(1216..1902)
                     /locus_tag="VGM72_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEY4264103.1"
                     /translation="MSNNTKKREPLRRTRPAKRAKRAAETKLDFQDPHAASSRRNSLE
                     LAIGREVRDFRRKLSMTVAELANMAGMSTGMLSKIENGQTSPSLATLQELSKALQVPV
                     TAFFRKFEEEQDATFVRAGQGLTIERRGTRAGHQYQLLGHSLSRNLAVEPYLITLTKD
                     SDVFPVFQHDGIEFIYMLEGEVGYRHGDRVYAMTPGDSLFFDADVPHGPEQLRKLPIR
                     FLSVITYLRS"
     gene            2191..2457
                     /locus_tag="VGM72_02200"
     CDS             2191..2457
                     /locus_tag="VGM72_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY4264104.1"
                     /translation="MNQKDLFAAVQKKSGVALKDVKSVVEAFFETVTKTSKKEPVRTT
                     LGTFVVAKRAARMGRNPQTGEAIKIAASKKFRFRPSKSTKEAVL"
     gene            2820..>3076
                     /locus_tag="VGM72_02205"
     CDS             2820..>3076
                     /locus_tag="VGM72_02205"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4264105.1"
                     /translation="MPPRAPNPMLIPMIIATALFMENLDSTVLATALPAIARSLNEDP
                     IRLSLAISSYLLSLAVFIPASGWFADRFGARQVFRAAILIFL"
BASE COUNT          579 a          920 c          914 g          663 t
ORIGIN      
        1 tatggccttc gatatggact tcgatcggct gggtgagtcc gaccgcgcag gcatgttcgc
       61 catgcggatt ggccagtttc cagagccgct cattggtttc cgccggaagg ccctgtagct
      121 tctgattgac ctcacgcagc gaggtctttt cgagatcgat gacgttcatg ctgcaacgac
      181 ccccaatgca acagtcgtca atgcaaactc catgtgtaaa cggtggcggg agccggctcc
      241 cagatcttcg ccttctcgat gttcggcaga taagcgaggc tgcggaattc ggacgccatc
      301 gccacccaat cgtcgttctc cgccagcacc gccggcttgc aggcgatgcc gtcgcgcacc
      361 acggcgaagc cgtccttggt gcccatggtg aaggtgaaga agccgtcgag atcgacgagg
      421 cccgcttcca gcgcttcttc gagcgtcgcg ccttcgctca ggcgataggc gaaatagcgc
      481 gcggccactt cggaatcatt gtcggtttcg aattcctgcc cctgatgccg cagccatttg
      541 cgcagattgt tgtggttcga cagcgagccg ttgtggacca ggcacatatc cgaagccggc
      601 gtgaagggat gcgaatgctc ggtggtgacg gcgctctcgg ttgccatccg ggtatggccg
      661 atcgcatggc tgccttccat cccggccagg ccgtaacgcg cggcgacttc cgcgggcagg
      721 cccatatcct tgtagacttc cataagcttg ccgtagccca tgatgcgcac gtcgggatgg
      781 ttggcgcgca accaaggcag caccttgtcc tcggagcaat gcgcgtgcag caaggcgtga
      841 ttgcccttct gctcgatctc ggttttcacc ttcaatgcct tggcaaggcc gtcggcgacg
      901 ccgcgccacg gataatggac gtccgcattg aagagggtga tcttggattg cccctcgggc
      961 acctcgctgt gatagacggc gatgccggcg ctgtccgggc cgcgctcgct catgcccacc
     1021 agcatgatct cgacatgctt gccgagctgc gattgcagcg caggattttt caggaaaaga
     1081 ccgacgatgc cgcacatggc gcgtctccgg ataggttcag gaattggcgc ggatactagc
     1141 ccagctattc cggccttaca agcggaggaa agtttttttc ctggcaggaa accccggtta
     1201 tgctggtatt cggggtcagc tccggagata ggtgataacc gacagaaatc ggatcggcag
     1261 tttccgaagc tgttccggcc cgtgcggaac gtcggcatcg aaaaacaggc tgtcgccggg
     1321 cgtcatcgca tagacccggt cgccgtgccg gtagcccact tcgccttcca gcatatagat
     1381 gaattcgatc ccgtcgtgct ggaacaccgg aaagacgtcg gaatccttgg tcaacgtgat
     1441 gagatagggc tccaccgcga ggttgcggct gagcgagtgg ccaagcagct gatactgatg
     1501 accggcccgg gttccgcgcc gctcgatggt caagccctgc ccggcacgaa cgaaggtggc
     1561 atcctgctct tcctcgaact tgcggaagaa ggcggtgacg gggacctgca gcgccttgga
     1621 cagctcctgc agagtcgcca gcgagggcga agtctggccg ttctcgatct tcgaaagcat
     1681 gccggtggac atgccggcca tattcgccag ttccgccacc gtcatgctca gcttgcggcg
     1741 gaagtcccgg acctccctgc cgatcgccag ttccaggctg ttgcggcggg aactggcggc
     1801 atgtggatct tgaaaatcga gcttggtttc cgcggcccgc ttggcgcgtt tcgccggtcg
     1861 ggtccggcgc agcggttcgc gttttttcgt gttgttcgac aaggttatgg cccagccgct
     1921 tgcatgcgcg ggcgctcaat tcgcccagga agtcaccgca ccagactaca caaaggcagt
     1981 cgctttcggc aatctttcgc tgccttgtgc gcctgcgcaa aattccgggg aggctcgagt
     2041 cgcatgcttt tatggcctct cgcgagtcgc gagaagatgc atttcgccgc ggttttcctt
     2101 gctcaatcga ttgctgacta cggccaattt gggtagcttg gattcgccgt gggcgattcg
     2161 cccgtacgcc aatcaacagg ggtctaagct atgaaccaga aagatctgtt cgccgccgtc
     2221 cagaaaaaga gtggcgtcgc cctgaaggac gtgaagagcg tcgtcgaggc tttcttcgaa
     2281 accgtcacca agacttccaa gaaagagcca gtgcgcacga cgctcggcac tttcgtagtc
     2341 gcgaagcgtg ccgcccgcat gggccgcaat ccgcagaccg gagaggcgat caagatcgcc
     2401 gcttcgaaga aattccgctt ccgtccgtcc aagtcgacca aggaagccgt gctctaaaaa
     2461 gaccggtgaa gcctaatagg cttcgctgca ccgccgaata cgccggcatc catggatgcc
     2521 agtgcttgat tggcagttgg gcaacgggtc gatttcgatc ggcccgttcc cagttgtcag
     2581 gttcgggttt gaattttcag cccgaaattt aaagctcaat ttcatttaaa gtttaatccg
     2641 gcgatccgct ttctcctccg catccgtcgc gtgagggggg caatattgac aaggggttag
     2701 cgcgctggct ccgaccggga atagaactcg ccgaagcatg ccatgcgcac gataaacgca
     2761 acgctagtgc gggtaattcg ttctattgtg gaacgctaat tcctcacgat catccggcca
     2821 tgcccccgcg cgcccctaat cccatgctca ttcccatgat catcgccacc gcgttgttca
     2881 tggagaatct cgattcgaca gtattggcga cggcgcttcc ggcgatcgcg cgatccctca
     2941 acgaagatcc catccggctt agcctggcga tatcgtctta tttgctgagc ctcgcggtct
     3001 tcattccggc gagcggctgg ttcgcggatc gcttcggggc gcggcaggtg tttcgcgccg
     3061 caattctgat cttcct
//
LOCUS       DASYEG010000058         5748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4512061, whole genome shotgun sequence.
ACCESSION   DASYEG010000058 DASYEG010000000
VERSION     DASYEG010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4512061"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VGM72_02210"
     CDS             <1..528
                     /locus_tag="VGM72_02210"
                     /inference="COORDINATES: protein motif:HMM:NF036421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264106.1"
                     /translation="IEAARVREWPALRRVVVAVDPPASAGADADECGIVIAGASADGH
                     GYVLADRSARGLSPLGWAKRAGAAYEAFSADAIVAEANQGGAMVGEVIRQASPDAVIR
                     LVHARRDKVTRAAPAAALYERGLVHHVGAFAELEDQLCQYDGTGASPDRMDALVWALA
                     NLFPGTAVKPRVRRI"
     gene            874..2076
                     /locus_tag="VGM72_02215"
     CDS             874..2076
                     /locus_tag="VGM72_02215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEY4264107.1"
                     /translation="MFERWFPWERPARVAAPETKNAPLIALQLSARAHWTSRDYVALA
                     REGVMANAVAYRAVRMIAEGAASVPWLLYEGQSELETHPLLDLLVTPNPDESGTELFT
                     NWYGHLQTAGNAYLEAVRLRGDVRELHVLRPDRVKVVAGARGAPDAYDYTVEGHTTRL
                     VRDAATGFLPVLQAKLFHPLDDHYGLSPIEAAAVAIDVHNAGAYWTKALLDNAARPSG
                     ALVYRGPDGQPNLTDEQFARIKQELESAYQGAANAGRPMVLEGGLEWRAMSYSPADMD
                     FAAARAAAAREIALAFGVPPMLLGIPGDNTYSNYREANLAFWRQTILPLVAKTAQALT
                     RWLAPGFGSALRVGYDSDAVDALAVEREAVWARINGADFLTLNERRAAAGYGPIPGGD
                     ESGNADGN"
     gene            2060..2356
                     /locus_tag="VGM72_02220"
     CDS             2060..2356
                     /locus_tag="VGM72_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264108.1"
                     /translation="MLTAIDMNERPARRRGFDFERRIPLALVLGFLLQTGGALFWAGS
                     AAQRISEIERETRANENAVERVVRLETEVAALHETLARIETKIDRMTAVRRDVR"
     gene            2369..2908
                     /locus_tag="VGM72_02225"
     CDS             2369..2908
                     /locus_tag="VGM72_02225"
                     /inference="COORDINATES: protein motif:HMM:NF016471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEY4264109.1"
                     /translation="MHRVERVKHRLTRKILPVRLSELGVDGFEGYASVFGARDGIGDI
                     VAPGAFAASLRRRGAGGVRMLYQHFAYEPIGVWDEIREDARGLHVRGHLVMEVERARD
                     VAALLREGALGGLSIGFKTMRARKDSATGSRTLQQVDLWEISVVTFPLLEAANVTAVG
                     GKTTALAQRIRDVTQLLQA"
     gene            3056..4300
                     /locus_tag="VGM72_02230"
     CDS             3056..4300
                     /locus_tag="VGM72_02230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEY4264110.1"
                     /translation="MELESKALNGPAESREFKEAFDEFRRTIEIFKAENDERLLKLER
                     RSEDAVTNEKMDRINQAVTEQKRALDEFVLASQRPQRGGLAVQTGGSAEHKSAFEHYV
                     RKGDAGGLAPFEIKSLSSQSDPDGGYLVPRETETAIDRVLAKVSPIRAIATVRQIGAQ
                     SYRKPVTTTGADAGWVGEAASRTETTAPSLSVVEFPTMELYAMPAATQILLDDAFINV
                     DQWLAEEVQTTFGEQEGAAFITGDGINRPRGLLSYTVVADASWAWNRIGYIASGGAGV
                     FASSNPSDALVDLVYAPRQSYRANGRWVMNRKTEAAIRKLKDGQGNYIWQPGASAGDP
                     ATLLGYPVHEAEDMPDITANSYSIAFGDFARGYLIVDRVGVRVLRDPYSAKPFVLFYT
                     TKRVGGGVQNFEAIKLMKFATS"
     gene            4342..4758
                     /locus_tag="VGM72_02235"
     CDS             4342..4758
                     /locus_tag="VGM72_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264111.1"
                     /translation="MRDLFNNITVARAIAPVSVSDNTAQVGQVIDRQGFSSLTFVINA
                     GSLADADATFAVLVEHGDAANLSDAATTAAGDLLGTAALAGFTFADDNATRKIGYKGT
                     KRYVRLTITPANNASAALFGALAILGHAQLAPTSNP"
     gene            4768..5337
                     /locus_tag="VGM72_02240"
     CDS             4768..5337
                     /locus_tag="VGM72_02240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HEY4264112.1"
                     /translation="MSVERIAAPASEPVSLAEAKAHLRVDNDDEDGLIARLIVAAREK
                     CESMTGRAFILQNWKFWCDAWPDCRAPRILTIPKPPLMSLTSVTAYDRSGAASLLSSD
                     AYIVDSAAAPGRLVLKDTANLPVDLREANAIAIAFQAGYGAEPSDVPAGIRAGILTLI
                     AHLYEGRGLSAGPPAETLALLAPFRVAML"
     gene            5334..5666
                     /locus_tag="VGM72_02245"
     CDS             5334..5666
                     /locus_tag="VGM72_02245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEY4264113.1"
                     /translation="MNAFPIGEARQRIVLETKVLTADGGGGYSENWEPYALVWAALEL
                     EGGGEPLQAGRPEMRVSCRIVIRRRSDVSVNHRVRLAARVFAIRAIVDPGAQSASMTL
                     LCEEGAPS"
BASE COUNT         1049 a         1771 c         1870 g         1058 t
ORIGIN      
        1 atcgaggctg cgcgcgtgcg cgaatggccc gcgctcagac gcgtcgtcgt tgccgtcgat
       61 ccgcccgcat cggccggcgc ggacgcggat gaatgcggca ttgtcatcgc gggcgcgagc
      121 gcggacggcc acggctatgt gttggcagac cgttcggcgc gcgggctttc gcctttgggc
      181 tgggccaagc gcgcaggcgc cgcgtatgag gcattttcgg ccgacgccat cgtcgccgaa
      241 gccaatcagg gcggcgccat ggtgggcgaa gtgatccggc aggcatcccc cgatgcggtg
      301 atccgtctgg tgcatgcgcg ccgcgacaag gtcacacgcg ccgctccggc ggccgcgtta
      361 tacgagcgcg ggctggtgca tcatgtgggc gcgttcgctg agctggaaga tcagctctgc
      421 caatatgacg gcacgggtgc gagtccggac cggatggatg cgctggtctg ggcgttggcg
      481 aatttgtttc ccggcaccgc cgtcaaaccc cgcgtgcgga ggatttagca aaccacaatg
      541 gccgcctctg ccgccatatt tgtcgacatt cgatcttcgc catcggtccg tggcgcaacg
      601 cgaatctgca tctgggaaaa ttcttcaagc tcgttcatga aagccaaaac attgaccggc
      661 aaattcaccc gaaaaagctc gtaaagatct ggcaatggca tggcgaaccc caatggactc
      721 agcgagtcct agggcctttc gggcatttgc gccataagac aaaaaactgt tctgctcact
      781 tgaacagtgg cgctgtcgtc accaagtttc ctacatcccc gtaataacaa acgcaccaat
      841 gtctcccgcg aagggagagg tgatggagca tccatgttcg aacgctggtt cccctgggaa
      901 cgtccggcgc gcgttgctgc gcccgaaacc aagaacgcac ctttgatcgc gctgcaacta
      961 tccgcgcgcg cgcattggac gtcgcgcgat tatgtggcgc tcgcgcgcga aggcgtgatg
     1021 gccaatgcgg tcgcctatcg cgcggtgcgc atgatcgccg aaggggccgc ctcggtgccg
     1081 tggctgcttt acgaaggtca aagcgagctg gagacgcatc cgcttctcga tctgctcgtg
     1141 acacccaatc cggatgagag cggcacggag ctgttcacca attggtatgg gcatcttcag
     1201 acggcgggca atgcctatct cgaagccgtg cgtctgcgcg gcgatgtgcg cgagctgcat
     1261 gtgctgcgcc ccgaccgggt gaaagtggtg gcgggcgcgc gcggcgcgcc ggatgcatat
     1321 gattacacgg tcgagggcca caccacgcga ctcgtgcgcg acgcggcgac cggattcctg
     1381 cccgtgctgc aggcgaaatt gtttcatccg ctcgacgatc attatggtct gtcgccgatc
     1441 gaagcggctg ccgtcgccat cgatgtgcac aatgcgggcg cctattggac caaggcgctg
     1501 ctcgacaatg cggcgcggcc ttcgggtgcg cttgtctatc gcgggccgga cggccagccc
     1561 aatctcaccg acgaacaatt cgcgcgtatc aaacaggaat tggagagcgc ctatcagggt
     1621 gcggccaatg cgggacggcc gatggtgctg gaaggcgggc tcgaatggcg cgccatgagc
     1681 tattcgccgg ccgatatgga tttcgcggcg gcgcgcgcgg ccgccgcgcg cgagatcgcg
     1741 ctcgctttcg gcgtgccccc gatgctgctc ggcattcccg gcgacaacac ctattccaat
     1801 tatcgcgagg cgaatctggc gttctggcgt cagacgattt tgccgctggt ggcaaagacc
     1861 gcgcaggcgt tgacgcgctg gctcgcgccc ggtttcggca gcgctttgcg cgtgggctat
     1921 gattcggacg cggtggacgc gctggccgtg gaacgcgaag cggtgtgggc gcgcatcaat
     1981 ggtgcggatt ttctcacgct taacgagcgg cgcgcggcgg cgggctatgg cccaattccc
     2041 ggcggcgatg aatctggcaa tgctgacggc aattgacatg aacgagagac cggcaaggcg
     2101 ccgcggcttc gatttcgaac gccgcattcc cttggcgctc gtgctgggct ttctgctgca
     2161 gaccggcggc gcgttgttct gggcgggctc ggcggcgcag cgcatcagcg agatcgagcg
     2221 cgagacccgc gccaatgaga atgcggtgga gcgcgttgtg cggctggaga ccgaggtcgc
     2281 cgcgctgcat gaaacgcttg cccgcatcga aaccaagatc gaccggatga cggctgtccg
     2341 ccgggacgtt cggtagaaga acatcatcat gcatcgtgtc gaacgcgtga agcatcgcct
     2401 cacgcgaaaa atcttgcctg tgcggctgtc ggaattgggc gtcgatgggt ttgaaggcta
     2461 tgcctcggtg ttcggcgccc gtgatgggat cggcgacatc gtggcgccag gtgcctttgc
     2521 cgccagtctc aggcggcgcg gcgccggcgg ggttcgcatg ctctatcagc attttgccta
     2581 tgagccgatt ggcgtgtggg acgaaatccg cgaggatgcg cgcggtcttc atgtgcgcgg
     2641 ccatctggtg atggaggtgg aacgcgcccg cgatgtggcc gcgctcttgc gtgaaggcgc
     2701 gctgggcggg ctttccatcg gtttcaagac aatgcgtgcg cgcaaagatt ccgcgaccgg
     2761 atcgcgcacg ctgcaacagg tggatctttg ggaaatttcg gtcgtgacgt ttccgcttct
     2821 tgaggcggcg aatgtgaccg ccgtcggcgg taagacaacc gcgctcgcgc aacggatccg
     2881 cgacgtcacc caattgctcc aagcatgatg aaaagatttc gcgggggacg tcttgaagcg
     2941 gcacgctgtc acgaccaagt agcttacatc ccgaaaatct caatcgcata gccggtgccc
     3001 gcgaacgccg gccgatgaat gctgtgcgca atgcgcaaac acataaggag acatcatgga
     3061 attggaatcc aaagccttga acggaccggc cgagagccgc gaattcaagg aagccttcga
     3121 cgaattccgc cgcaccatcg agattttcaa ggcagagaat gacgagcggc tgctgaaact
     3181 ggagcgccgc tccgaagatg ccgtcaccaa cgaaaagatg gatcgtatca accaagcggt
     3241 gaccgaacaa aagcgcgcgc tcgacgaatt cgtgctcgcg agccagcgtc cccaacgcgg
     3301 cggcctggcg gtgcaaaccg gcggcagcgc cgaacacaaa tcggccttcg aacattatgt
     3361 gcgcaaaggc gatgccggcg gtctggcgcc gttcgagatc aaatcgctct cctcgcaatc
     3421 cgatcccgat ggcggctatc tcgtgccgcg cgagaccgaa accgccatcg accgtgtgct
     3481 cgccaaggtg tcgcccattc gcgccatcgc gaccgtgcgg cagatcggcg ctcaatccta
     3541 tcgcaagccc gtcaccacca cgggcgcgga tgcaggctgg gtcggcgaag cggcctcgcg
     3601 caccgagacc accgcgccga gcctttccgt cgtcgaattt ccgaccatgg agctttatgc
     3661 catgccggcg gcgacccaga tcctgctgga cgatgccttc atcaatgtcg atcagtggct
     3721 ggccgaggaa gtgcagacca cgttcggcga gcaggaaggg gcggccttca tcaccgggga
     3781 tggcatcaat cgtccgcgcg gcctcttgag ctacaccgtg gtcgccgatg cgagctgggc
     3841 ctggaacaga atcggttaca tcgcctcggg cggggcgggc gtgttcgcgt cctccaatcc
     3901 atccgatgcg ctggtcgatc tggtctatgc cccgcgtcag tcgtatcgcg ccaatggccg
     3961 ctgggtgatg aaccgcaaga ccgaagcggc gatccgcaaa ttgaaggacg gccagggcaa
     4021 ttacatctgg caacccggtg cgtcggccgg cgatcccgcg accttgctgg gctaccccgt
     4081 gcacgaagcc gaggacatgc cggacatcac ggccaacagc tattcgatcg cgttcggcga
     4141 tttcgcgcgt ggctatctga tcgtcgaccg cgtcggcgtg cgcgtgttgc gcgatcccta
     4201 ttcggccaag ccgttcgtcc tcttctacac caccaagcgc gtggggggcg gcgtgcagaa
     4261 tttcgaagcg atcaagctga tgaagttcgc gacctcctga accgaaaatt tttacccaaa
     4321 gatatttgca aggaacaaga catgagagat ctgttcaaca acatcaccgt cgcgcgcgcc
     4381 atcgcgccgg tgtcggtgag cgacaatacg gctcaggtgg gtcaggttat cgaccgccag
     4441 ggcttcagca gtctcacttt cgtgattaac gcgggcagcc tcgccgatgc ggatgcaaca
     4501 ttcgccgtgc tggtcgagca tggcgatgcg gccaatctgt ccgatgccgc gaccacggcc
     4561 gcgggcgatc tcctcggcac ggcggcgctc gctggcttca cctttgccga cgacaatgcg
     4621 acgcgcaaga tcggctacaa gggcacaaag cgctatgtgc ggctgacgat cacaccggcc
     4681 aacaatgctt cggctgcact gttcggcgcg ctggcgattt tgggccacgc gcaactcgct
     4741 ccgacctcca acccgtaaaa cgcacccatg tctgtggaac gcatcgccgc tccggccagc
     4801 gagcctgtct cgcttgccga ggcgaaggcg catttgcgcg tggacaatga cgatgaggac
     4861 gggttgattg cccgcctcat cgtcgccgcc cgcgagaaat gcgaaagcat gaccgggcga
     4921 gcgttcatcc tccagaactg gaagttctgg tgcgatgcct ggcccgattg ccgggccccg
     4981 cgcattttga ccatccccaa gccgccactg atgagcctca catcggtgac ggcgtacgac
     5041 cgctccggtg cggccagttt gctgtccagc gatgcctata tcgtggatag cgcggccgcg
     5101 ccggggcggc tcgtgctcaa ggatacggca aacttgccgg tcgatctgcg cgaggccaac
     5161 gccatcgcga ttgcgtttca ggccggctat ggcgcggagc cttccgatgt gccggctgga
     5221 atccgcgccg gcatcctgac gctcatcgcg catctgtacg aggggcgtgg actgtccgcg
     5281 ggcccgccgg ccgagacgct ggcgctgttg gctccgttcc gcgtggcgat gctgtgaacg
     5341 cctttcccat cggcgaagcc cgtcagcgaa tcgtactgga aaccaaggtt ctgaccgccg
     5401 atgggggcgg gggatattcg gagaattggg aaccctatgc gctggtttgg gccgcgctcg
     5461 aactcgaagg cggcggcgaa cccttgcagg ccggccgtcc cgagatgcgc gtctcgtgcc
     5521 ggatcgtcat ccgccggcgc agcgatgtct ccgtcaatca ccgcgtgcgt ctggccgcgc
     5581 gggttttcgc catccgcgcc atcgtcgatc cgggggcgca atcggcgagc atgacattgc
     5641 tgtgcgaaga aggagcgcca tcatgaccga agtgttttcc tttgcccggc ttgatgcggg
     5701 gattgcgcgc gccgttcagg aaggcgcgcg cgatgccgca ggccagct
//
LOCUS       DASYEG010000059         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1323797, whole genome shotgun sequence.
ACCESSION   DASYEG010000059 DASYEG010000000
VERSION     DASYEG010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323797"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VGM72_02250"
     CDS             <1..375
                     /locus_tag="VGM72_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264114.1"
                     /translation="RAKTGAARAKMKSPRAKAGVTRSKKTAARGKAAKTRTKTMMGRA
                     KAAPGSVRKAASRGAKSAMAAERTLVKAAKNMVSAATPATGAKRGRKKNTVASIAARA
                     RRKNAAATDYNPLHMLAKLSPF"
     gene            complement(361..867)
                     /locus_tag="VGM72_02255"
     CDS             complement(361..867)
                     /locus_tag="VGM72_02255"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEY4264115.1"
                     /translation="MTALITLIVAVAENGVIGVKGGLPWYIPEDLKRFKALTLGKPII
                     MGRKTWDSLPKKPLPGRTNIVVTRDSIFHADGAILAHSFDDAVAVAEREHPQEIMVIG
                     GQAIFAAALPRAHRIHWTDVMTRPEGDAFMPPLDPAVWKETAREGPFESPGDSGGLRY
                     AFLVLERR"
     gene            complement(905..1699)
                     /locus_tag="VGM72_02260"
     CDS             complement(905..1699)
                     /locus_tag="VGM72_02260"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEY4264116.1"
                     /translation="MRQYHDLMEWVLGHGLEKRDRTGTGTLSVFGHQMRFDLNDGFPL
                     LTTKKLHVKSIIHELLWFLRGDTNVRYLRENGVRIWDEWADDAGELGPVYGKQWRSWP
                     KWDGSSIDQIALVVQQIMRNPDSRRLIVTAWNPSDIEEMALPPCHCLFQFYVGEGRLS
                     CQLYQRSADIFLGVPFNIASYALLTMMVAQVTGLKPGEFIHTFGDAHLYRNHLEQARE
                     QLTRRPYPLPTMKLNPPVKDIFAFKFEDFMLENYQAHPHIKAAVAV"
     gene            complement(1778..2074)
                     /locus_tag="VGM72_02265"
     CDS             complement(1778..2074)
                     /locus_tag="VGM72_02265"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEY4264117.1"
                     /translation="MKIEFNPAKSAKKRKPLTDRDGEVRELTAADFRTMKRLDHFPEL
                     AKLVRGRGERGPQKTPTKQQVTLRLDRDVVERFRATGEGWQSRINEALRKARVG"
     gene            2111..>2432
                     /gene="ssrA"
                     /locus_tag="VGM72_02270"
     tmRNA           2111..>2432
                     /gene="ssrA"
                     /locus_tag="VGM72_02270"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
BASE COUNT          513 a          747 c          717 g          455 t
ORIGIN      
        1 cgagccaaaa cgggcgctgc gcgcgccaaa atgaaatcgc cgcgcgcaaa agccggtgtg
       61 acccgttcaa agaaaaccgc agcgcgtggc aaagccgcca agacacgcac caagacgatg
      121 atgggacgtg cgaaagcggc ccctggaagc gtgagaaaag ccgcatcgcg tggcgccaaa
      181 agcgccatgg ccgcggaacg cacgctcgtc aaagcggcga aaaacatggt gagcgcggca
      241 acacccgcca ccggcgccaa gcgcggccgc aagaaaaata cggtcgcctc catcgccgcg
      301 cgggcgcgcc gcaagaacgc ggcggcaacc gattacaatc cgctgcacat gctggcgaaa
      361 ttatcgccgt tctaatacga ggaacgcgta gcgaaggccc ccggaatcgc ccggcgattc
      421 gaacgggcct tcgcgcgcgg tttccttcca gacagccggg tcgagcggcg gcatgaacgc
      481 atcgccttcg ggccgggtca tgacatccgt ccagtggatg cgatgcgcgc gcggcagagc
      541 ggcggcgaaa atcgcctggc cgccgatcac catgatctct tggggatgtt cgcgctccgc
      601 gacagcaacc gcatcgtcaa aggagtgcgc caggatcgcg ccatccgcgt gaaaaatgga
      661 atcgcgcgtc actacgatgt tggtgcggcc ggggagcggc tttttcggca ggctgtccca
      721 ggtcttgcga cccatgatga taggcttgcc cagcgtcagc gccttaaagc gcttcaaatc
      781 ttcgggaatg taccagggca atcccccctt gaccccgatg acgccgttct cggcgaccgc
      841 gacaatcagc gtgatgagag ctgtcatatc ccctcctcat cctgaggagc gctcgcagcg
      901 cgcatcatac cgccaccgcg gccttgatgt gcggatgggc ctgataattc tccagcatga
      961 aatcttcgaa tttaaacgcg aaaatatcct tcaccggcgg attcaatttc atggtgggca
     1021 gcggataggg ccgccgcgtc aattgttcgc gcgcctgttc gagatggttc cgatagagat
     1081 gcgcatcgcc gaaggtgtgg atgaactcac cgggtttcag ccccgtcact tgcgccacca
     1141 tcatcgtcaa gagcgcatag gacgcgatgt tgaacggcac gcctaagaag atgtccgccg
     1201 aacgctgata gagctggcag gaaagcctgc cctccccgac atagaactgg aacaggcaat
     1261 ggcagggcgg cagcgccatc tcctcgatgt ccgagggatt ccaggccgtg acgataagcc
     1321 gccgcgaatc gggattgcgc atgatctgct gaacgaccag cgcgatctga tcgatggagc
     1381 tgccgtccca tttcggccag cttcgccatt gcttgccgta aaccggaccg agctcacccg
     1441 catcatccgc ccactcgtcc cagatgcgca cgccgttctc ccgcagatag cgcacattgg
     1501 tgtcgcccct gaggaaccag agcagctcgt ggatgatcga tttgacgtgc agcttcttgg
     1561 tggtgagaag cgggaagccg tcgttgaggt cgaagcgcat ctgatggccg aaaaccgaaa
     1621 gcgtgcccgt gccggtgcgg tcgcgcttct ccagcccgtg cccgagcacc cattccatca
     1681 gatcgtgata ttgccgcatg tcacagtggt aaaagcgatc acgctgccga gtctagcgcc
     1741 ttctgagcat tgaaatgaaa taaccgctac aactcgatca ccccacccgg gctttgcgca
     1801 gcgcctcgtt gatacggctc tgccagccct cgcctgtcgc gcgaaagcgt tcgacaacat
     1861 cccggtccag ccgcagggtg acctgctgct tggtcggcgt cttttgcggc ccgcgttccc
     1921 cgcggccccg cacgagtttg gccaattcgg gaaaatgatc caagcgtttc atggtgcgaa
     1981 aatcggcggc cgtcagttcg cgaacctcgc cgtctctgtc agtcagcggt tttcttttct
     2041 tcgcgctctt ggcgggattg aattcgattt tcacggattt atttgtagct acaaaaagaa
     2101 agaccggcaa gcgctttaag tcttaaccgc acatccctat attgggtgtg tccgggttta
     2161 cccggactat ggcgataaac ggcctcgtaa taagcggccc ggacccgggg gcggtacccg
     2221 gcgcctccac caaatgcccg tcgaagcctc atcctaggct aggttccggc gggcatctgt
     2281 gggggcgaaa caggatcgac ggacgtgtaa aggtggttct ttcgctcggt atggtatccg
     2341 ccgttatcgg gctattcgta caggtgccaa cgataatgaa atggccctcg ctgcttaatt
     2401 aatacctaag caagcgcggc tcgaggggcg cc
//
LOCUS       DASYEG010000060         6099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3787785, whole genome shotgun sequence.
ACCESSION   DASYEG010000060 DASYEG010000000
VERSION     DASYEG010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3787785"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VGM72_02275"
     CDS             <1..372
                     /locus_tag="VGM72_02275"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HEY4264118.1"
                     /translation="PEQIRPENDRSILKYGYGLTTAVARPTRSAVEFSRDELVRAGAV
                     LERRIAAFKPRHVAFLGKMAYAAIVKNRDIPWGPQTGTFASAPVWVLPNPSGLNRNFQ
                     RDALVCAYRDMRVAVMDKIVN"
     gene            445..885
                     /locus_tag="VGM72_02280"
     CDS             445..885
                     /locus_tag="VGM72_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY4264119.1"
                     /translation="MPALLRHARTAYGAAMRRALEESGYDDIPKNGLYVIGGLALGVG
                     DAPLGQLIRELGVSKQAAGHLIDALVLRGYLERNVDDEDRRKLTLALTARGRAAAATQ
                     AAARQKIDAELMACVGEEDVRRTRRTLAILANMRSTDKAGDAGH"
     gene            981..1907
                     /locus_tag="VGM72_02285"
     CDS             981..1907
                     /locus_tag="VGM72_02285"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4264120.1"
                     /translation="MPRSTAFAIAFSLLSGTAHAAAYDDAFVAYRKGDYQLALKLMKP
                     LAEAGNVNAQYNVGVMYNENLVKQDYAEAAKWFQKAAAQGNVFAEVDLGTLYEYGRGV
                     KTDYKEALRLFRLAADKKNPTAEFSLGSMAERGLGVPQDYKEAVKWYQLAANQGNADA
                     QYDLALMLMAGRGVPQDYKQAASWYRKSADAGNASAQLNLGALYYAGQGVPKAPKEAL
                     KYFRLAAQQGNGRAQFNLATLYTNGDEGVAQDRPRALIWFNAAAVTLTGNDAKIADEN
                     GKAVAAQMTREQIRAAQELGHKCQAGDYKSCD"
     gene            2030..3271
                     /locus_tag="VGM72_02290"
     CDS             2030..3271
                     /locus_tag="VGM72_02290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEY4264121.1"
                     /translation="MLDMLLARLIEQGTLTVIWPGGKSRTYGTGGRRVTTRLHGLSTP
                     WAIGLRPELAFGEAYMDGRLTVEEGTIADVLEIIISNLGMRPLPRIVRFARALRRSFR
                     WIVQFNPASRARRNVAHHYDLSGALYDLFLDSDRQYSCAYFKEPKMTLEAAQSAKKRH
                     IAAKLKLDRPDLSLLDIGSGWGGMGLELARGAGANVLGITLSTEQLALSNSRAAAAGL
                     AERCRFAFKDYRDIQGPFDRIVSVGMFEHVGIMHYDTFFRKMVCLLAPDGVALLHTIG
                     RTDGPGTTNSWITKYIFPGGYTPALSEVMAAIERSGLYATDVEILRLHYAETLKEWRL
                     RFRKNWAKAVALYDECFCRMWEFYLASAEMTFRYNQLVVFQIQLARRIDTLPMTRDYM
                     LDGERRMGLAPEQLSRANEAA"
     gene            3429..4865
                     /locus_tag="VGM72_02295"
     CDS             3429..4865
                     /locus_tag="VGM72_02295"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF006606.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEY4264122.1"
                     /translation="MDQEISRQLPYDVDIEQALLGAILVDNYSLERVSSLLKPEHFYD
                     PLHQRLFDAVERMWAKGHAVTPLTLKATMEQDAGLAEVGGQAYLISLARAAPALPNVK
                     DYARILADLAMRRELIRIGEDIVNTAFEASIDVAPQSQVEEAEKALYRVSEKARFGEG
                     PLSFDTALAQAVAYAEQALARGGQISGVPSGFADLDSLLGGLNASDLIIIAGRPGMGK
                     TSLATNMAFHAARLRAKDKADGATPPRGAPVLLFSLEMAASQLSARILSEQTEIEMRK
                     IRTGRFTDAEWDRFVQTAQVLSDLPLYIDDTGGISIAQIAARARRLKRERQIGCVIID
                     HIQLVAPSRRVENRVQEITEISKGLKVLAKELDVPVIALSQLSRGVDNRDDKRPILSD
                     LRESGSIEQDADVVMFVYREEYYLQSREPEAGTSEYDDWVKKLERVHNRAEVLVEKNR
                     HGPTNKVELFFDARFTRFSNLAGDGERR"
     gene            complement(4900..5871)
                     /locus_tag="VGM72_02300"
     CDS             complement(4900..5871)
                     /locus_tag="VGM72_02300"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY4264123.1"
                     /translation="MIRQALVTLTIALAAGLSVSSAVAQTPPNTVQLQPAPDIPGVIA
                     GGTMPEVVIKGLTSSDDPFWLPNVGLIFSESQANRIVRLDEHDQPVTFVGNLLNPLGM
                     TLDAKGQLISIQTRPGFLGPRIVWPKGKERIIADNYQGKPFGRPNDIVADLKGGLYFT
                     DPPASAVYYVPAGGAIIRVVNEGIKSPNGLQLSHDEKTLYVNDTRGINIYAFDVQPDG
                     RLANRRVFATYTGRDMSLPPTAPPVSRADGLVTDDEGRLYALTDSGIEVLSPTGQHLG
                     AIPVWCITRRCQNLGFGGPDKRTLYVAGGGTLLRIAMRARGFLGRAK"
     gene            complement(5976..>6099)
                     /locus_tag="VGM72_02305"
     CDS             complement(5976..>6099)
                     /locus_tag="VGM72_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEY4264124.1"
                     /translation="IENYFCRIKDWRRIATRYDKLARNYLAAAAIVGALVWIKL"
BASE COUNT         1186 a         1806 c         1922 g         1185 t
ORIGIN      
        1 ccggaacaga tacgtcccga gaacgatcgc tcgatcctga aatatggcta tgggctgacg
       61 accgccgtgg cgcgtcccac acgaagcgca gtcgagtttt cgcgcgacga acttgttcgt
      121 gcaggcgcgg tgctcgaacg caggatcgcg gcgttcaagc cgcgccacgt cgcctttctc
      181 ggcaagatgg cctacgcggc catcgtcaaa aatcgcgata ttccttgggg gccgcaaacc
      241 gggacattcg cgagtgcgcc ggtatgggtg cttcccaatc cgagcggcct caaccgtaac
      301 ttccaacggg atgcgctcgt ctgtgcctat cgcgacatgc gcgtggcggt aatggataaa
      361 atagtaaatt aacttgacta taaaatatct gtatagtaca gtcgtgagat ggaatcaaag
      421 aggccctggt tcgaagatat cgtcatgccc gccttgcttc ggcatgcgcg cacggcctat
      481 ggcgcggcga tgcgcagggc tttggaggaa tcgggttacg atgatattcc caaaaacggg
      541 ctctatgtga tcggcgggct ggccttgggc gtgggcgatg cgccgctggg acaattgatc
      601 agggaacttg gcgtctccaa gcaggctgcg ggccatttga tcgatgcgct tgtgctgcga
      661 ggttatctgg aacgcaatgt ggacgatgag gatcgccgca agctgacatt ggccctgacc
      721 gcgcgcggcc gcgcggccgc ggcgacgcag gccgcggctc ggcaaaagat agatgcggaa
      781 ttgatggcct gcgtcgggga agaggacgtg cgccggaccc ggcgcaccct tgcgatcctc
      841 gccaacatgc gcagcacgga caaggctggc gacgcgggtc attgagggct gcgcgatgtc
      901 gtgaacgcag gcacgagccg agctgagact ttcggaccgc ggcaaacggc gttaggattc
      961 agctcgcttg agggcgcgcc atgccgagat cgaccgcatt tgccattgca ttctcgctct
     1021 tgagcgggac ggctcacgcc gccgcctatg acgatgcctt cgttgcctat cgcaaaggcg
     1081 attatcagct cgcgctcaaa ctgatgaagc ctttggcgga agccggcaat gtgaacgccc
     1141 aatacaatgt cggcgtcatg tacaatgaaa acctagtgaa gcaggactat gccgaggcgg
     1201 cgaaatggtt tcagaaggcg gccgcccaag gcaatgtctt cgcggaagtg gatctcggaa
     1261 cgctttacga atatggccgc ggtgtgaaga ccgattacaa ggaagccctg aggctgttcc
     1321 ggctggccgc cgacaagaaa aatcccaccg ccgagttcag tctcggttcc atggccgagc
     1381 gcgggctcgg ggtaccgcag gactataagg aagcggtcaa atggtatcag ctcgccgcga
     1441 accagggcaa cgcggacgcg caatacgatc tggcgctgat gcttatggcc ggccggggtg
     1501 tgccgcagga ctataaacag gccgcttcct ggtatcgtaa atccgccgat gcgggaaacg
     1561 ccagcgcaca actcaatctc ggcgcgctct attatgcggg gcagggtgtt ccgaaggccc
     1621 caaaggaagc gctgaaatat ttccggctgg cggcacagca gggaaatggg cgcgcgcagt
     1681 tcaaccttgc cacgctctat accaacggcg atgaaggcgt tgcccaggat cgcccccgtg
     1741 cgctgatctg gttcaatgcg gccgcggtga cgcttacggg caatgacgcc aagatcgccg
     1801 acgagaacgg caaggcggtc gcggcccaga tgacccgtga gcagattcgg gccgctcagg
     1861 aactcggcca caaatgccaa gctggcgatt acaaaagctg cgattgattc tggtgtctgg
     1921 gcggatcgag cggaaccgta cataaaatca cagtgttcgg ctctttctgt ccggacccct
     1981 tcgacgctgg ttttcgttaa gaaagttgaa atttccggcg gatacgtcga tgctcgacat
     2041 gcttcttgcg agactcatcg aacagggtac gctcaccgtc atctggccgg gtggaaaaag
     2101 cagaacctac ggcacggggg ggcggcgggt gacaacccgc ctccatggct tgtcgactcc
     2161 ctgggcaatc gggctgcggc ccgaactggc gttcggcgaa gcctatatgg atggccgcct
     2221 gaccgtcgag gaaggcacca tcgccgatgt cctcgagatc atcatctcaa acctgggcat
     2281 gcgcccgctg cccaggatcg tgcgtttcgc gcgcgccctt cgccgctcct tccgctggat
     2341 cgtacaattc aatccggcct cgcgcgcacg ccggaacgtc gcgcatcatt acgatctgtc
     2401 gggagcgctc tacgatctgt tcctggattc cgaccggcaa tattcttgcg cctatttcaa
     2461 ggagcccaag atgacgcttg aggccgccca atcggccaag aagcgtcata tcgcggcaaa
     2521 gctgaagttg gaccggcccg atctttcgct gctcgacatc ggttcgggct ggggggggat
     2581 ggggctggag cttgcacggg gcgccggcgc gaacgtcctg ggcatcacac tgtccaccga
     2641 gcaattggcg ctgtccaaca gccgcgccgc cgctgcgggt ctcgccgaac gctgccgttt
     2701 cgcattcaag gattatcgcg acattcaagg gccgttcgac cgtatcgtct cggtcggcat
     2761 gttcgagcat gtgggcatca tgcattacga cacattcttc cggaagatgg tttgccttct
     2821 cgcgccggat ggcgtggcgc ttctgcacac gatcggccgc accgacgggc cgggcacgac
     2881 caatagctgg attaccaaat atattttccc gggcggctat acgccggcct tgagcgaagt
     2941 catggctgcc atcgagcgtt cgggccttta cgcaaccgac gtggaaatcc tgcgcttgca
     3001 ctatgccgag acgctcaagg aatggcgtct gcgtttccgc aagaattggg cgaaggcggt
     3061 ggcgctctat gatgagtgct tttgccggat gtgggagttt tatcttgcct cggcggaaat
     3121 gacgttccgc tacaatcagc tggtggtgtt tcagatccaa ctcgcccggc gcatcgacac
     3181 gcttcccatg acgcgcgact atatgctcga tggcgaacgg cggatgggtc tggcgccgga
     3241 acagctttcg cgcgccaacg aggcggcctg acttttcgca attggcctgg ctcttcgcaa
     3301 ttgtggataa cgcactgcaa caagcggttg cgaaaaatca atcctgtgag tcgcggaata
     3361 tattgcttgt gccggcttgt cagaacgaca attgattcgg ccctcgcgca aaccaaccgc
     3421 cggacttcat ggatcaggaa atctcacgtc agcttcctta cgatgtggac atcgagcagg
     3481 cgcttctcgg tgcgatcctg gtcgacaatt attcccttga gcgcgtgtcc tcgctgctca
     3541 agcccgagca tttctacgat cccctgcatc agcggctctt tgacgccgtc gaacgcatgt
     3601 gggcgaaagg tcacgccgtc acgccgctga cgctcaaagc cacgatggag caggacgcgg
     3661 ggctcgccga ggtcggcggt caggcctatc tcatcagtct ggcgcgcgcg gcacctgccc
     3721 ttcccaatgt gaaggattac gcgcgtatcc tggccgatct cgcgatgcgc cgcgaattga
     3781 tccgcatcgg cgaggatatc gtcaacaccg cgttcgaagc ctctatcgac gtcgcgccac
     3841 agtcgcaggt ggaggaggct gaaaaagcgc tctaccgcgt gtcggaaaag gcgcgcttcg
     3901 gtgaaggccc gctgtccttc gacaccgcac tggcacaggc tgtcgcctat gcggagcagg
     3961 cgctggcgcg tggcggtcag atttccggtg tgccatcggg cttcgccgat ttggactcgc
     4021 ttctaggcgg gctgaatgcc tccgatctca tcatcatcgc ggggcggccg ggcatgggca
     4081 agacctcgct tgccaccaac atggcattcc acgccgcgcg tctcagggcc aaggacaagg
     4141 ccgacggcgc cactccgccg cgcggcgcgc cggtcctgtt gttctcattg gaaatggcgg
     4201 cctcccaatt gtcggcgcgc attctctcgg agcagaccga gatcgagatg cgcaaaatcc
     4261 gcacgggccg cttcaccgat gccgaatggg atcgcttcgt gcagaccgcg caagtcttga
     4321 gcgatctgcc gctctatatc gacgacaccg gcggcatttc catcgcccag atcgcggcac
     4381 gggcgcggcg gttgaaacgc gaaagacaga tcggctgcgt gatcatcgat catatccagc
     4441 ttgtcgcacc gtcgcgccgg gtcgagaatc gggtgcagga aatcaccgag atcagtaagg
     4501 gcctgaaagt gctggccaag gaattggacg tgccggtgat cgcgctctcg caattgtcgc
     4561 gcggcgtgga caatcgcgac gacaagcggc ccatcctgtc ggatttgcgc gaatccggct
     4621 ccatcgagca ggacgcggac gtcgtcatgt tcgtctatcg cgaggaatat tatctacagt
     4681 cgcgcgagcc cgaggccggg acgtcggaat atgacgactg ggtcaagaag ctggagcgcg
     4741 tgcacaaccg cgccgaggtt cttgtcgaaa agaaccgcca cgggcccacc aacaaggtgg
     4801 agttgttctt cgatgcccgc ttcacacggt tctcgaacct ggccggtgac ggcgagcgcc
     4861 gataagcgac ggcgacagga atgtcattcc cggctctgcc tattttgcgc ggcccaggaa
     4921 tccgcgcgcg cgcattgcga tgcgaagcag cgtgccgccg cccgccacgt agagcgtgcg
     4981 cttgtcgggg ccgccgaatc ccaaattctg gcagcgccgc gtgatgcacc agaccgggat
     5041 ggcgccaagg tgctgtccgg ttgggctcaa gacctcgatg cctgaatccg tcagcgcata
     5101 cagccggccc tcatcatcgg tgaccagccc gtcggccctc gacacgggcg gcgccgtcgg
     5161 gggcagcgac atgtcgcgcc ccgtataggt ggcgaacacc cggcgattgg cgaggcggcc
     5221 gtccggctgc acgtcgaagg catagatgtt gatgccccgg gtgtcgttca catagagcgt
     5281 cttttcatcg tgactgagct gcaggccgtt tggagacttg atcccttcgt tcaccacgcg
     5341 gattatcgcg ccgccggcgg gcacgtagta cactgcggag gccggagggt ccgtgaagta
     5401 caggccgcct ttaaggtccg ccacgatgtc gttcggacgg ccgaagggct ttccctggta
     5461 gttgtccgcg ataattctct ccttcccttt cggccacacg attcgaggtc cgaggaatcc
     5521 gggtctcgtc tggatcgaaa tgagctggcc cttcgcgtcg agggtcatgc cgagcggatt
     5581 gagcagattg cccacgaagg tgacgggctg gtcgtgctcg tccagcctca cgatgcggtt
     5641 cgcctgcgac tccgagaaga tgaggccgac gttgggcagc caaaaggggt cgtcggagct
     5701 cgtcaatccc ttgatcacca cctcgggcat cgtgccgccg gcgatgacgc ccggaatgtc
     5761 gggagccggt tgcagttgaa cggtgttggg cggtgtctgc gcaacggccg aggagacgct
     5821 caagcctgcg gccagcgcga tggtcagcgt cacaagcgct tgtcggatca tggcaacggc
     5881 tccttcgcgg gtcgagacga ccatccgcat gatgcgagtt tacggcatat cggggccggt
     5941 agcgaatgtc cgctttggat gactagggtc tggactcaca acttgatcca gacgagcgcg
     6001 ccgacgatgg cagcggcggc aaggtaattt ctggcgagtt tgtcataccg ggttgcaatg
     6061 cgcctccaat ccttgatgcg gcagaagtag ttctcgatc
//
LOCUS       DASYEG010000061         3731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4802247, whole genome shotgun sequence.
ACCESSION   DASYEG010000061 DASYEG010000000
VERSION     DASYEG010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4802247"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VGM72_02310"
     CDS             <1..304
                     /locus_tag="VGM72_02310"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264125.1"
                     /translation="ASVHAMNGMLMDAKGMTLYTWDNDKQANKSSCNGMCQMNWPALK
                     AEASDKDMGDWKVIARDDGSKQWAYKGKPLYYFSMDAAAGDKKGEGRGMVWHMAKM"
     gene            520..681
                     /locus_tag="VGM72_02315"
     CDS             520..681
                     /locus_tag="VGM72_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264126.1"
                     /translation="MKFFSKFFAGRLIVTTAMSAATAAIAVTISGADSTSVFPVYSKW
                     AAVDRVSQA"
     gene            804..1793
                     /gene="pstC"
                     /locus_tag="VGM72_02320"
     CDS             804..1793
                     /gene="pstC"
                     /locus_tag="VGM72_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEY4264127.1"
                     /translation="MVNLALSQDLSSSSNSAVLRRFHAGDRTFRRIALASAFLLLAMF
                     AGILGALTIGAWPAVRAFGLPFLWTESWNPATEKFGALAPIYGTLVTSTIAMLISVPV
                     GIGIAIFLTELCPRSLRRPIGVAVELLAGIPSIIYGIWGLFVLAPFLQQYVMPGFIAA
                     FSNVPVLSSLFAGPPFGIGIFTAGLILGIMTLPFISAITRDVFETVPPVLKEAAYGLG
                     ATTWEVQRNVVLPYARSGVVGGVMLALGRALGETMAVTFVIGNAHRIAASLLAPGTTI
                     SAAIANEFTEAIGELYEPALIELGLILFFLTASVLAVAQYMLYRLELRAGGRS"
     gene            1790..2638
                     /gene="pstA"
                     /locus_tag="VGM72_02325"
     CDS             1790..2638
                     /gene="pstA"
                     /locus_tag="VGM72_02325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEY4264128.1"
                     /translation="MTGNRRYLRRRARSGIFLGLSAAATLFGLGWLALILGTLIHEGL
                     SGVDLAIFTQSTPPPGAMGGLLNAIFGSVLMSSFAVAIGTPIGIFAGTYLAEYGRHTR
                     FAFVVRFINDILLSAPSIVLGLFVYDVMVLSLGHFSAWAGGVALATIVVPVVVRTTEN
                     MLLLIPNSLREAAAALGAPRSHVIRQVTWRAARAGIITGVLLAIARISGETAPLLFTA
                     LNNQFWSVNPNAPMASLPVAIFQMAMSPYADWHRLAWSGALLITMTVFILSIVARILE
                     AQTGRR"
     gene            2635..3408
                     /gene="pstB"
                     /locus_tag="VGM72_02330"
     CDS             2635..3408
                     /gene="pstB"
                     /locus_tag="VGM72_02330"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEY4264129.1"
                     /translation="MTIPDNETVKVAINDLSFFYGDTKALKNISLPLYANKVTAFIGP
                     SGCGKSTLLRVMNRIYELYPNQRAEGEVLLDGENILSRKLDVNALRAKVGMVFQKPTP
                     FPMTIFDNVSFGLQLYESLPRSEMEGRVELALRRAALWDEVKDKLHASGTSLSGGQQQ
                     RLCIARSVAIKPELILLDEPCSALDPISTAKIEELINELSDDYTIVIVTHNMQQASRV
                     SDHTAFMFLGELIEFGETEKVFTTPTNKRTEDYITGRFG"
     gene            3454..>3731
                     /locus_tag="VGM72_02335"
     CDS             3454..>3731
                     /locus_tag="VGM72_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009315099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEY4264130.1"
                     /translation="MSGDIRNDWTKDEVRALFALPFGELIFRAQTLHRKYFDTREVQI
                     STLLSIKTGGCPEDCAYCPQSAHYETGVKAEKLIALSAVLDAAETAKAS"
BASE COUNT          701 a         1099 c         1065 g          866 t
ORIGIN      
        1 tgccagtgtc catgcgatga atggcatgtt gatggatgcc aagggcatga ctctctacac
       61 ctgggacaat gacaagcaag ccaacaaatc ttcatgcaac ggcatgtgcc agatgaactg
      121 gccggcgctg aaggccgaag cctccgataa ggatatgggc gattggaagg tcatcgcccg
      181 cgatgatggc tccaagcaat gggcttacaa gggcaagcct ctctattatt tcagcatgga
      241 tgccgccgcc ggcgacaaaa agggcgaagg ccggggcatg gtttggcata tggccaaaat
      301 gtaaggcctc ttgcagcctt tttcttcggg gcgccggtga tgccggcgcc cttttctttg
      361 agtgccctct tctttgaatt tccccgcggc cgggtcctgc tgtcataaaa gcttcgtgca
      421 aacgtgacat cgcttgcgcg cgatttggca ttgtccgccg gtcggcgcag ccgaaggtct
      481 gccgccgcca cggcgccgca aggaactcaa gaggatcgaa tgaaattttt cagcaagttc
      541 ttcgctggaa ggctgatcgt gacgaccgcc atgagcgcag caactgccgc catagccgtg
      601 acgatttccg gcgccgactc gacatccgtg ttccctgttt attccaaatg ggcagccgtg
      661 gatcgcgttt ctcaagccta gaggggcggc gacaaccggt acccttcgca aaactcgtca
      721 aattggcaaa tatgggagtg ggcgacccgc gttcggaaaa cggtgcccgc tcaatatttg
      781 agaaccccat ctccgagtct ctcatggtca atcttgcttt gagccaagac ctttcctctt
      841 cgtccaattc cgcggtgttg cggcggtttc atgcaggcga tcgcactttt cgccggatcg
      901 ccttggcttc cgcgttcctc cttcttgcga tgttcgcggg aatcctggga gctctgacga
      961 taggcgcttg gcccgcggtc agggcgttcg ggctcccctt tctatggacg gaaagctgga
     1021 acccggcgac cgaaaaattt ggtgcgctcg cgccgattta tgggacgctc gtgacctcga
     1081 cgatcgcaat gctgatttcg gttccggtgg gaatcggaat cgcgattttt ctgaccgagc
     1141 tttgtccccg ttccctgcgc cggccgattg gcgtggccgt cgagctgctc gccgggatcc
     1201 ccagcatcat ctacggaatt tggggacttt tcgtcctcgc gccatttctt cagcaatatg
     1261 tcatgccagg ttttatcgcg gctttttcta atgttcctgt gctatcgagc ctatttgccg
     1321 gtcccccgtt cggcatcggc attttcacgg ccggacttat cctcggaatc atgacactgc
     1381 cctttatcag tgcgatcacg cgcgacgtgt tcgaaaccgt cccccccgtc ttgaaggaag
     1441 ccgcttacgg cctcggcgcc accacttggg aagtccaacg gaatgtggtg ctgccttacg
     1501 cccgtagcgg tgtggtcggc ggcgtaatgc tggctctggg ccgcgccctc ggcgaaacca
     1561 tggcggtgac attcgtgatc ggaaacgccc atcgtatcgc ggcttctctg ctcgcgcccg
     1621 gcacgacgat atcggcggcc attgccaatg agttcaccga agcgatcggc gaactctatg
     1681 aaccggcatt gatagaactc ggcctcattc ttttcttcct cacggcaagc gtgctggccg
     1741 tggcgcaata catgctctac cggttggaac ttcgcgcagg aggccgttca tgaccggaaa
     1801 ccgccgctat cttcgcagac gcgcgcgaag tggcattttt cttggtcttt cggcggcagc
     1861 caccctgttt ggcctgggtt ggctcgcgct gatccttggg acgctcatcc atgaagggtt
     1921 gtccggcgtc gatttggcga tctttaccca atcgacgccg ccgccaggag ccatgggcgg
     1981 actgcttaat gccattttcg ggagtgtgct gatgagcagt ttcgccgtcg ccatcggtac
     2041 accgatagga atttttgctg ggacgtatct ggcggaatat ggccgccaca cgcggttcgc
     2101 ttttgtggtg cgtttcatca acgacattct gctgtcggcg ccttccatcg tgcttggtct
     2161 ctttgtctac gatgtcatgg tgctttcgct gggtcacttc tcggcctggg ccggtggcgt
     2221 ggcgcttgcc acgattgtcg tccctgtggt cgtgcgcacc acggaaaaca tgctgctgct
     2281 gattcccaat tcattgcgtg aagccgccgc cgcgctcggc gcgccgcgct cgcacgtcat
     2341 ccggcaggtg acttggcgcg cggcgcgggc cggcatcatt accggtgtcc tgcttgcgat
     2401 cgcgcgcatc tcgggagaga cggcgcctct tcttttcact gcgctcaaca atcaattctg
     2461 gagcgttaat cccaatgcgc cgatggcaag ccttccggtt gcgattttcc agatggccat
     2521 gagcccctat gcggattggc acaggctcgc ctggtcgggc gctcttctta tcaccatgac
     2581 ggtgtttatc ctgtccatcg tcgcacgcat actcgaagcg caaacgggtc gcagatgacc
     2641 ataccagaca atgaaaccgt caaggtcgcg atcaacgatc tttcgttttt ttatggcgat
     2701 acgaaagcgc tgaagaatat ctcccttccg ctgtatgcca acaaggtgac cgcgttcatc
     2761 gggccatcgg ggtgtggcaa gtccacgctg cttcgtgtca tgaaccgcat ctacgaactt
     2821 tatcccaatc aacgcgcgga aggcgaagtg ctgctggacg gggagaacat cctttcccga
     2881 aagctcgacg taaacgctct gcgcgccaag gtcggcatgg tgtttcagaa gccgacgcct
     2941 ttcccgatga cgattttcga caatgtctcg ttcggattgc agctttacga aagtctgccg
     3001 cgttcggaga tggaagggcg agtcgaactc gcactccgcc gcgcagcact ttgggacgag
     3061 gtcaaagaca aactccatgc cagcggtacg tcgctttccg gcggccagca gcagcggctt
     3121 tgtatcgcgc gttcggtggc gatcaagccc gagctgattc tgctggatga gccctgttcc
     3181 gcgctggacc cgatatcgac cgccaagatc gaggagctga tcaacgagct ttccgacgat
     3241 tacacgatcg tcatcgtcac ccacaatatg cagcaggcaa gccgcgtttc cgaccacacc
     3301 gccttcatgt tcctgggtga actgatcgag ttcggcgaaa ccgagaaagt ctttaccacg
     3361 cccaccaaca aacggactga ggattacatc accggccgct tcggttgagg agccggtgct
     3421 tgcgttttgc gcgcatcacg cgatagtgcc gccatgagcg gcgatatccg aaacgattgg
     3481 acgaaggacg aggtgcgggc gctctttgcg cttcccttcg gcgagttgat tttccgcgcc
     3541 cagacattgc accgcaaata tttcgatacg cgcgaggtgc agatttcaac gctgctttcg
     3601 ataaaaaccg gcggttgtcc cgaagattgc gcctattgcc cgcaaagcgc ccattacgag
     3661 accggcgtga aggcggaaaa gctgatagcg ctttcggccg ttctcgatgc cgcggaaacg
     3721 gcgaaagcat c
//
LOCUS       DASYEG010000062         7355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3788098, whole genome shotgun sequence.
ACCESSION   DASYEG010000062 DASYEG010000000
VERSION     DASYEG010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 7355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7355
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3788098"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..156)
                     /locus_tag="VGM72_02340"
     CDS             complement(<1..156)
                     /locus_tag="VGM72_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY4264131.1"
                     /translation="MGKQKFERTKPHCNVGTIGHVDHGKTTLTAALTRVSADRGWTST
                     AISYDQVA"
     gene            complement(239..312)
                     /locus_tag="VGM72_02345"
     tRNA            complement(239..312)
                     /locus_tag="VGM72_02345"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(278..280),aa:Gly,seq:tcc)
     gene            complement(425..509)
                     /locus_tag="VGM72_02350"
     tRNA            complement(425..509)
                     /locus_tag="VGM72_02350"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(473..475),aa:Tyr,seq:gta)
     gene            862..1629
                     /locus_tag="VGM72_02355"
     CDS             862..1629
                     /locus_tag="VGM72_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY4264132.1"
                     /translation="MSRQKTRSEAKSSPSEGAFWLYGLHAARAALANPRRKIRKAVLS
                     ERAQAEFGPALARAKVAAVSQASSDNISRLLPPGAVHQGVALVCEPLPALHLEDALSS
                     TPPGPRLVAVLDQITDPHNEGAILRSAAAFGVAAILVQDRHAPPESGALAKAASGGLD
                     LIPRIAVVNIARALEELGRLGFYRIALAADGDGPLREAAPAGDIALVLGAEGSGLRRL
                     VRERCDASAFIPMGGVMESLNVSNAAAIAFYELRRKP"
     gene            complement(1809..2690)
                     /locus_tag="VGM72_02360"
     CDS             complement(1809..2690)
                     /locus_tag="VGM72_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEY4264133.1"
                     /translation="MTREALSYQGVYINLERSTARRARMEAQLAQLKLTDLYVRFPAV
                     DGAKLPLPGSKIGPGETGAFLSHMRVLEEARQRGLPLHVLEDDALLCAPVRPVIGDVI
                     ASGLLDRFDIVFTDTLLMPHLGMLKGLKAEFDRVMRPQTRPLCFADLKAIDLKRENFS
                     CLTSYVVGAGAMDRILALYRAELARGPLKPVDLFLRDCVADGRLRAGLLFPFVTSFRL
                     DEVAPSTVAAGTQNAKPSVMVLAVLRYLFFVERDLVYAKSCLDAVTQQNRRKSDPHHD
                     LMVQALEFVLSADFQPF"
     gene            2811..4457
                     /locus_tag="VGM72_02365"
     CDS             2811..4457
                     /locus_tag="VGM72_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY4264134.1"
                     /translation="MTDDNSRATIARQDADNQPPPFEDVNLFASDRVLQEALAREGGS
                     NHRARAETFGARMGSAEIWALAAAAHHHTPELHSHDRYGRRIDEVEFHPAYHAMMREG
                     IGAGISSAAWSGIQAGHVLHATLEFLLAEVEPSVCCPITMTYAAPAALKHAPGIAAEW
                     IPRILSQTYDSASRPAALKAGVTIGMAMTEKQGGSDVRANTTAAERLCDGTYRLIGHK
                     WFCSAPMSDAFLTLAQAEGGLTCFLCPRWTPDGTRNTIHLMRLKDKLGDRANASAEIE
                     YHGAYALRIGDEGRGIATILDMVHHTRLDCAIAPAAYMRSALAQALWHTAHRHAFGKA
                     LIEHTPMRQVLADLAIESESATVLAFRVARSFDESLQDKESALFSRLATPVAKYWLNK
                     RGIAHLAECLECHGGAGYVEEWPIARFYRQAPLNGIWEGSGNVICLDVLRALGRDSQS
                     SEVFLNEVRRAKGGNPHLDHAIAKLESQLAQTGESNARLLVERMALVLQGALLLRHAP
                     SFVADAFCTTRLGREGGQAYGVLDVRIDTNAIIARAMPEH"
     gene            complement(4461..6704)
                     /gene="parC"
                     /locus_tag="VGM72_02370"
     CDS             complement(4461..6704)
                     /gene="parC"
                     /locus_tag="VGM72_02370"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEY4264135.1"
                     /translation="MGDISFKDDIRPEPLGKALSERYLAYALSTITSRALPDARDGLK
                     PVHRRLLHAMRELGLNPAAGFKKCARVVGDVIGKFHPHGDQAVYDALVRLAQDFAVRY
                     PLVEGQGNFGNVDGDNAAAMRYTESRLTAVAEALLAGIDEDSVDFRPTYDGEDREPVV
                     LPGAFPNLLANGATGIAVGMATSIPPHNAGELCQAIVALIENPNVQDRTIFRHVKGPD
                     FPTGGLIIDDPATIQEAYRTGRGGFRVRARWQREEGARGTYQIVVDEIPYQVQKAKLI
                     ERIAQLIEEKKLTALDDVRDESTEDIRIVLVPKSRTVEPEILMEQLFRETDLETRVPL
                     NLNVLDKGVSPRVMSLREALTAFIDHRRDVLGRRSRFRLAKIAERLEVLEGYLAVYLN
                     LDKVIKIIRSEDEPKPKLIAAFKLTDRQAEAILNMRLRSLRKLEEMQIREEHTALAKE
                     QKDLKRLLGSEKQQSEKLIEEVKDIDRRFGVKTELGKRRTVIAEAKEVEKILATAEEV
                     AEMITAVEREPITIVCSDKLWIRTLKGHQEQGDSVKFREGDRGRFWLHAETTDSLMLF
                     ATDGRFFTLDCAKLPGGRGLGEPLRDFLDLPPEADMVAMFVHREGRKLLLAATSGHGF
                     VTSEDDAVAMTRSGKRVMNVPPGIEAVTCRFVEGDSVAVLGENRKLLIFALEEVPELA
                     RGRGVILQRHKDGHLLDAQVFTWKEGLRDANGRTFTPAELKEYRGQRAQAGRITPRGF
                     SKANRLG"
     gene            6886..7242
                     /locus_tag="VGM72_02375"
     CDS             6886..7242
                     /locus_tag="VGM72_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264136.1"
                     /translation="MFAKSIAMHIAIVGALNLGAAAAPAIADPFFFGTGGPDGKIAAG
                     SRSGPGFEIETADDFIVTQVATLNSATFTGLIPAGSSVLNVVVEIYRVFPSDSDVTRT
                     SGSPLFSTSLGSGLTT"
     gene            complement(7232..>7355)
                     /locus_tag="VGM72_02380"
     CDS             complement(7232..>7355)
                     /locus_tag="VGM72_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEY4264137.1"
                     /translation="IENYFCRIKDWRRIATRYDKLARNYLAAAAIVGALVWIKL"
BASE COUNT         1434 a         2289 c         2185 g         1447 t
ORIGIN      
        1 cgccacctga tcgtacgaga tcgccgtcga cgtccagccg cgatcggccg aaacccgcgt
       61 cagcgccgcc gtcagagtcg tcttgccgtg gtcaacgtga ccaatcgtgc caacgttgca
      121 gtgcggcttc gtgcgttcaa atttctgctt gcccatgacc taacctctta ttcttcttta
      181 aagcccgtct tcgccgtcat tctcaaaacc ggcttgccaa gtcgtagccg ccgaagactg
      241 gagcgggtga agggaatcga accctcgtca tcagcttgga aggctgttgc tctaccattg
      301 agctacaccc gcgatgaacg gaaaatcgac caacagaagc caggactcga tctccgaagg
      361 gtggctcatc tcctgtgaca ctcccgtctt tttccgtttc gtcttctgac ttccattttc
      421 cgattggtgg gggaggtagg actcgaacct acgaaggctg cgccagcgga tttacagtcc
      481 gccccctttg ccactcggga cactcccccg tttgatgcca gagaatggcg ccaaaacccg
      541 ttcaatagtg accgcatctc cctgaattaa caccaaaaaa ccggaggcca cgaacacgtc
      601 ggcacaccgg caaagcggga tcgctccgga ggaaactgcg gagggcgcat attggtgaat
      661 tgcggcttac tgtcaataga ggttgacaag agattgcggc gcgcggccat tggcatcgca
      721 ccggctcctc taacctgatg aaagacgcct aacgcgaccg ccgaaaagcg caagcggggc
      781 agagacattg atgaatagca ttggaatttc tgaacaccgc cttctcatgc cctctgcgga
      841 atgcttgccg aaggcgcacg catgagccgc caaaagaccc gttccgaggc aaagtccagt
      901 ccgtccgaag gagcgttttg gctttacggg ctgcacgccg cccgggccgc gctggccaat
      961 ccccgtcgca agatccgcaa ggccgtcctg agcgagcgag cgcaggctga attcggcccc
     1021 gccttggcca gggccaaggt tgccgctgtc tctcaagcca gctctgacaa tatttcacgg
     1081 ctgctgccgc cgggcgccgt ccatcagggc gtggcgctcg tctgcgagcc tctgccggca
     1141 ctccatctgg aggacgcgct ttcgagcacc ccgccgggtc ccaggctcgt cgcggttctc
     1201 gaccagatca cagacccgca caatgaagga gcgattctgc gttcggcggc cgccttcggg
     1261 gtggcagcaa tccttgtgca ggaccgccac gcgcccccgg aatcgggcgc cctggcgaaa
     1321 gccgcgtccg gcgggctcga cctgattccc cgcatcgcgg tcgtcaacat cgcgcgcgcc
     1381 ctcgaagaac tcggccggct tggcttctat cgcatcgcgc ttgccgccga tggcgatggt
     1441 ccgcttcgcg aggccgcgcc tgcgggcgat atcgctttgg ttctcggcgc cgaaggttcg
     1501 ggcttgcgtc gtctggtgcg cgaacgctgc gatgcgagcg cgttcatccc catgggcggc
     1561 gtcatggaaa gtttgaacgt gtcgaatgcg gcggcgatcg ccttttacga attgcgccgc
     1621 aagccctagc tgaagcgatc acatcgccgt gcggaacaag tcttcacctt cgccattatg
     1681 taacccagag gaacaacaac gaggccagaa atgcgctccg tgcttctttg ggctattggc
     1741 ataccgatcc cgatcatcat tattctgtgg ctcgtgacgg gccacgcctg acgtcaaaat
     1801 tgttgaaatc agaatggttg aaaatctgcg gatagaacga attccagcgc ctgaaccatc
     1861 aaatcatggt gtggatcgga cttccgccga ttttgttgcg taacggcatc gagacatgac
     1921 ttcgcataga cgagatcgcg ctccacgaaa aacagatagc gcaacaccgc gaggaccatc
     1981 accgagggtt tggcgttttg cgttccggct gcgacggtgc tcggcgccac ctcatccaga
     2041 cgaaaactcg tgacgaacgg aaagagcagg ccggcgcgca ggcgtccgtc cgccacgcaa
     2101 tcgcgcagga aaagatcaac cggcttcagc ggcccacgcg ccagctcggc acggtagagc
     2161 gcgaggatgc gatccatcgc gccggcgccc accacatagg aggtgaggca cgagaaattt
     2221 tcgcgcttga gatcgatcgc tttaagatcg gcgaagcaca gtgggcgtgt ttgcggcctc
     2281 atcacgcgat cgaactccgc cttcaatcct ttgagcatac cgagatgggg catcagcagc
     2341 gtatcggtga agacgatgtc gaacctatcc agaagacccg aagcgataac atcgccgatc
     2401 accggacgca caggggcgca tagaagcgca tcgtcttcga gaacatgcaa aggcaggccg
     2461 cgttgccgcg cctcttccag aacgcgcatg tgcgacagga acgcacccgt ttcgccgggt
     2521 ccgattttgc tcccgggaag cggcagcttc gctccatcga cggcgggaaa ccgcacatag
     2581 agatcggtga gctttagttg cgcgagctgt gcctccatgc gcgcgcgccg tgccgtgctg
     2641 cgctcaagat tgatatagac gccctggtag gagagagctt ctcgcgtcat cctggcagcc
     2701 ggttgaacac ggtttaacgg cttacccggc gcgagacgcc aagtccacat cgtagccgtg
     2761 gaaaatcatt tccagacagc atctgatagg atctctgcgg gagatgcgcc atgaccgacg
     2821 acaattcgcg cgcgaccatc gcgcgtcagg acgcggacaa tcagcccccg cctttcgagg
     2881 atgtgaatct gttcgcgtcc gatcgcgtgc tgcaagaggc cctggcgcgc gaaggcggtt
     2941 cgaatcatcg cgcccgcgcg gaaacattcg gcgcgcgcat gggctccgca gagatatggg
     3001 cattggccgc ggctgctcat catcatactc cggagcttca cagccacgac agatatgggc
     3061 ggcgaatcga tgaggtggag tttcaccccg cctatcacgc gatgatgcgc gaggggatag
     3121 gcgcagggat ctcatcggcg gcatggtccg gcatccaggc cggtcatgtg ctgcacgcaa
     3181 cgctggaatt cctcctggcc gaggtggagc cttccgtctg ttgtcccatc acgatgacct
     3241 atgccgcgcc tgcggctctg aagcacgcgc ccggcattgc cgctgaatgg attccgcgca
     3301 tcctcagcca aacctacgat agcgcgtcac ggcccgcggc gctgaaagcc ggcgtgacaa
     3361 tcggcatggc gatgaccgaa aaacagggcg gctcggatgt gcgcgccaac acaacggcgg
     3421 ccgagcgtct ttgcgacgga acctatcggc tgataggaca caaatggttt tgctccgcgc
     3481 cgatgtccga tgcatttctc acattggcgc aggccgaagg cggattgaca tgttttctct
     3541 gtccacgctg gacgccggat gggactcgta acaccattca tctgatgcgg ttgaaggaca
     3601 agcttggcga ccgcgccaac gcatcggccg aaatcgagta tcacggcgcc tatgcactcc
     3661 ggatcggcga tgagggccgt ggcatcgcga ccatcctcga catggtgcat cacacgcgcc
     3721 tggattgcgc catcgcgccc gccgcttaca tgcgcagtgc gcttgcccag gcgctgtggc
     3781 acaccgccca ccgccatgcc ttcggcaagg ccctcatcga gcatacgccg atgcgccaag
     3841 tgctggccga tctcgcgatc gaatccgaaa gcgcaaccgt gctcgccttt cgtgtcgcgc
     3901 gcagtttcga tgaatcgcta caagacaaag agtccgccct gttttccagg ctcgcgacgc
     3961 cggtcgcaaa atattggctc aataaacggg gaatcgctca cctcgcggaa tgcctggaat
     4021 gtcacggcgg tgcaggctat gtcgaagaat ggccgatcgc gcgtttctat cggcaggctc
     4081 cgctcaacgg aatctgggaa ggttccggca acgtcatatg cctggacgtg ctgcgcgcgc
     4141 tcgggcgcga ttcccaatcc agcgaggtct ttctgaacga ggtgaggcgc gccaaaggcg
     4201 gcaacccgca tctcgaccac gcgatcgcga aactggagtc gcagttggcg caaaccggcg
     4261 aatccaacgc ccgccttttg gtggaacgca tggcgcttgt gctgcaaggg gcgctcctgt
     4321 tgcggcacgc accgtctttt gtggccgatg cgttctgcac gacacgtctg ggcagagaag
     4381 gcggccaagc atatggcgtg ctggacgtta gaatcgatac gaacgcgatc atcgcgcgcg
     4441 caatgccgga gcattgacca tcatcccagc cggtttgctt tcgagaagcc gcgcggggtg
     4501 atgcgccccg cctgcgcgcg ctgaccccta tattccttca attcggccgg cgtgaaggtg
     4561 cggccgttcg catcgcgcaa gccttccttc caggtgaaaa cctgtgcatc gagcagatgc
     4621 ccatccttgt ggcgctgaag gatcacgccg cgcccgcgcg ccagctccgg cacctcttcg
     4681 agcgcgaaga tcaggagctt gcggttctca ccgagcaccg caacgctatc gccttcgacg
     4741 aaacggcatg tcaccgcttc gattccgggc ggcacattca tcacgcgctt gcccgaacgc
     4801 gtcatcgcca ccgcgtcgtc ttcactcgtg acgaacccat gtccggacgt tgccgcgagc
     4861 agcagcttgc gtccctcgcg atggacgaac atcgccacca tatcggcctc cggcggcagg
     4921 tcgagaaaat cgcgcagcgg ctcgcccaga ccgcgtccgc cgggaagctt ggcgcaatcc
     4981 agggtgaaga agcgcccgtc ggtggcgaac agcatcaggg aatcggtggt ctcggcgtgc
     5041 agccaaaacc ggccgcggtc gccttcgcga aacttgacgg aatcaccttg ctcttgatga
     5101 cccttgagcg tccgtatcca aagcttgtcc gagcagacga tggtgatcgg ctcgcgttcc
     5161 accgcagtga tcatctcggc gacttcttca gccgtggcaa ggattttctc cacctccttc
     5221 gcctccgcga tcacggtgcg gcgcttgccg agctccgtct tcacaccgaa gcggcggtcg
     5281 atatccttga cttcctcgat caatttttcc gactgctgct tttccgagcc gagcagtcgc
     5341 ttcagatcct tctgttcctt cgcaagggcc gtatgctcct cgcggatctg catttcctcg
     5401 agcttgcgca aactgcgcag ccgcatgttg aggatcgcct cggcctgacg gtcggtaagc
     5461 ttgaatgccg cgatcagttt cggctttggc tcgtcctcgc tgcggatgat tttgatcacc
     5521 ttgtcgaggt tcaaatagac ggcgagatag ccctccagca cttccagccg ctcggcgatc
     5581 ttcgccagtc gaaaccggct ccggcggccg aggacatcgc ggcgatgatc gatgaacgcc
     5641 gtcagcgcct cgcgcaggct catcacgcgg ggcgaaacac ccttgtcgag cacgttgaga
     5701 ttgaggggca cgcgcgtttc cagatcggtc tcgcggaaca attgctccat caggatctcg
     5761 ggctcgacgg tgcgactctt gggcaccagc acgatgcgga tgtcctcggt ggattcgtcg
     5821 cgtacatcgt cgagcgccgt aagtttcttt tcctcgatca gttgggcaat gcgttcgatc
     5881 agcttggcct tctggacctg atagggaatc tcatccacga cgatctgata ggtgcctcgc
     5941 gcgccttcct cgcgttgcca gcgcgcgcga acgcgaaaac cgccgcggcc ggttcgataa
     6001 gcctcctgaa tcgtggcggg atcatcgatg ataagcccgc cggtggggaa atccggacct
     6061 ttgacgtgcc tgaagatcgt gcggtcctgg acattcggat tttcgatcag cgcaacaatg
     6121 gcctggcaca actcgccggc attgtgcggg ggaatcgacg tcgccatgcc aaccgcaatc
     6181 ccggtggcgc cattggccaa gagattggga aaagcgccgg gcagcacgac cggctcgcgg
     6241 tcctcgccat cataggtggg ccggaaatcc accgaatctt cgtctatgcc tgcgagcagc
     6301 gcttcggcca cggccgtgag gcggctttcg gtgtaacgca tggcggcagc gttatcgcca
     6361 tcgacgttcc cgaaattgcc ctgcccctcc acgagcggat agcgcaccgc gaaatcctga
     6421 gccagacgga cgagtgcgtc atagaccgct tgatcgccgt ggggatggaa tttgccgatg
     6481 acgtcgccga cgacgcgagc gcatttcttg aaacccgcag ccgggttcag ccccagctcc
     6541 cgcatcgcgt gcaacaggcg gcggtgtacc ggcttcaatc cgtcgcgcgc atcgggaagc
     6601 gcgcgcgagg taatggtcga gagcgcatag gcaaggtagc gctcggatag cgccttgccc
     6661 aagggctcgg gacgaatgtc atccttgaac gatatgtcac ccatgttttc tctttagtcc
     6721 gattcggctg aggcgcggac ggctagatga gtgagtcgcg ccggcggtat ggggtacgcg
     6781 tcacctggcg aattctggtc caaacggcac tttgacctgg cctaaattat gcttaaagtt
     6841 gagctgtggg tttcaatctt cctgacgttt caagggtgaa acgcattgtt cgctaaatcc
     6901 atcgccatgc atattgcgat tgtcggggcg cttaacctgg gggcagcggc ggcgccggct
     6961 atcgctgacc catttttctt cggcaccggc ggccccgacg gtaagatcgc cgcggggtcg
     7021 cgatcgggcc cgggattcga gatcgaaacg gccgacgact ttatcgtgac ccaggtcgcg
     7081 acactgaaca gcgccacatt caccggtttg attcctgccg gcagttccgt gttgaacgtg
     7141 gtcgtcgaaa tttatcgcgt ttttccaagt gattccgacg taacgcgcac gagcgggtcc
     7201 ccgctgttct cgacgagcct agggtctgga ctcacaactt gatccagacg agcgcgccga
     7261 cgatggcagc ggcggcaagg taatttctgg cgagtttgtc ataccgggtt gcaatgcgcc
     7321 tccaatcctt gatgcggcag aagtagttct cgatc
//
LOCUS       DASYEG010000063        11830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2628876, whole genome shotgun sequence.
ACCESSION   DASYEG010000063 DASYEG010000000
VERSION     DASYEG010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 11830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11830
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2628876"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VGM72_02385"
     CDS             <1..435
                     /locus_tag="VGM72_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264138.1"
                     /translation="SPHLIDRYSEALAWLALDDPQLDRIRRELLNLAASGKSLDKAAV
                     ENHLVRQGLGVLAERLKTHSVLQSDLRGQADEEAREVLWLRTRAQLADPDISGAGDLK
                     ARRDSALQRYLDGGANQDWDELQRLNGEIRKAVEADGKRDVK"
     gene            432..2954
                     /gene="rpoD"
                     /locus_tag="VGM72_02390"
     CDS             432..2954
                     /gene="rpoD"
                     /locus_tag="VGM72_02390"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEY4264139.1"
                     /translation="MSKKTPSKAKPVLKPTKVKHEAAKPAVKQREPARAAAAKTAIKK
                     APSKSKVEVKKPEIKKPIAKQSGPEPRLAAKLAAAKPPAKEIPKAAAVKIPEPKSAKA
                     EPAKPAGPKPALPVELKGHPAQKLDPKSAAKKNGTAPEGAQAPAPDKGDPDAPLLDLS
                     DAAVRKMITRAKQRGYVTYDELNRVLPSDKVSSEQIEDTMAMLSEMGINVIESEEQEE
                     AEGEQLPAVQAGRAVAEVPEKAEETYDRTDDPVRMYLREMGSVELLSREGEIAIAKRI
                     EAGRELMIGALCEGPLTFEALTVWRDELMEGKALLRDIIDLEAMYGGVPGAEGALGQG
                     IAPPGAPGMPGVPPGPGVPGVPGAVPPAPPPVMKAPAPPPPQPVAAKPAPSRMGPADD
                     ITGGDGGATPEGGGEGGGDEDFDDESNLSLSAMEAALKPQVLATLDQIAALYKKLGKL
                     QDAQVEAALGAEEFSTGQERRFVKLRNETVELVKSLRLNNNRIEALVDQMYGINRRLL
                     SLEGRLLRLAEAHGVDRTDFLKQYFTNELDPNWVRRVGRLTGTGWKDFAHEERDKIKD
                     LRDTIQALAQETRQSISDYRRIVQTVQKGERESRQAKKEMIEANLRLVISIAKKYTNR
                     GLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVH
                     MIETINKLVRTSRQMLHEIGREPTPEELAERLAMPLEKVRKVLKIAKEPISLETPIGD
                     EEDSHLGDFIEDKNAILPIDAAIQSNLRETTTRVLATLTPREERVLRMRFGIGMNTDH
                     TLEEVGQQFSVTRERIRQIEAKALRKLKHPSRSRKLRSFLDN"
     gene            3051..3326
                     /locus_tag="VGM72_02395"
     CDS             3051..3326
                     /locus_tag="VGM72_02395"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HEY4264140.1"
                     /translation="MPTIAYFYGIAIRMYFEDHDPPHFHAIYAGNEAFVSIATGEIFE
                     GRLPKNAARLVKEWTLARQSLLMDNWLNARSGKPVKKVPGLDADQSD"
     gene            3307..3576
                     /locus_tag="VGM72_02400"
     CDS             3307..3576
                     /locus_tag="VGM72_02400"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HEY4264141.1"
                     /translation="MLTKVTRVEAIGDFHLRTHFSDGSCGEHDFSALAHKPGEMLMPL
                     RDPSYFARVFLEDGAPTWPNGYDIAPEWLRREMEAAGELHRAAAE"
     gene            complement(3698..3988)
                     /locus_tag="VGM72_02405"
     CDS             complement(3698..3988)
                     /locus_tag="VGM72_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEY4264142.1"
                     /translation="MNMKHPLHPGLSVRHDCLEPLNLTVTEAAKALGVTRQALNNLVN
                     GKSGISPEMAIRLDKAFGGGAEIWLGLQLDYDLAEARKSERGIKVRRIPRAA"
     gene            4372..4743
                     /locus_tag="VGM72_02410"
     CDS             4372..4743
                     /locus_tag="VGM72_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264143.1"
                     /translation="MKPTIARVALFVAGFAVWAAFSIVPGSDGGFTTREAWDSAPYWE
                     FGVPLLAAAQAISAAASREKLLQLPVWPLAGHFLGMFAIHRAGADFALVPLAFVFIGV
                     PGYFTLLAVSVAARKFAGRFA"
     gene            complement(4961..5512)
                     /locus_tag="VGM72_02415"
     CDS             complement(4961..5512)
                     /locus_tag="VGM72_02415"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY4264144.1"
                     /translation="MALVQNPICEFGWKAKDFSLPGVDGKTHSLNDARGPNGTLAVFI
                     CNHCPYVRAIVGRFVEETHALKALGIGVIAIMPNDVKISLGDSFDAMTLFAREHDFTF
                     PYVMDRTQDVARAYGAVCTPDFFGFNAKDELQYRGRLDESKTSLVRNARRDLYEAMKG
                     IAETGHGPKDQIASMGCSIKWRE"
     gene            complement(5584..6045)
                     /locus_tag="VGM72_02420"
     CDS             complement(5584..6045)
                     /locus_tag="VGM72_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264145.1"
                     /translation="MGIFVAERSTDFYSDFEHATKDSTIIEKGGTSGRIRLKAAGNRL
                     HVIIEQEPAGGCLIACFREGLERKILCPNMRALADVTGFTGSVDWDAIHAIGAMADWG
                     EGGDSRVAYVVRNDQFAPLIKVLQVLFAKTHHQSFTNRKDATAWLESRDPA"
     gene            6284..6703
                     /locus_tag="VGM72_02425"
     CDS             6284..6703
                     /locus_tag="VGM72_02425"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HEY4264146.1"
                     /translation="MAKASMGQRKTEGRIMHEFKHGELKSGKSGKGGKVKSRKQAIAI
                     ALHEAGASKYDSPAKRKQNLAKTKGKEAKGETAQQEREGKSRVGARGKRENTSAMGGK
                     NAKTPTRRAKSKGRATVSRPGTARKISRASGRKRSSA"
     gene            complement(6704..8497)
                     /locus_tag="VGM72_02430"
     CDS             complement(6704..8497)
                     /locus_tag="VGM72_02430"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HEY4264147.1"
                     /translation="MGLSEAVNREAAYIAAVARTLMLTRGVRPDARRSIADWTERWAR
                     ERPASPAILYEDRVVSWEALDAGANRFARWALAIGLRKGDVVSLLMENRPEYVMAWLG
                     LIKSGMIAALINTHLTGQPLAHCIQIAGAKHLILGEELAANYATALAGLDPRPVLWTT
                     GGARAGCENLDAILSSQSDAPMSAGSRSDIFCRDKALYIYTSGTTGLPKAANISHMRL
                     LHIMSAFQGAVNAGPDDRMYDVLPLYHSAGGIAALGPSFLGGGSLVVRSHFSATEFWD
                     DCHRYRPTLFQYIGELCRYLLNTPEHPYERDHRLRIAIGNGLRPEIWPKFQARFKIPR
                     IMEFYGATEGNVGMINYDGKVGAIGRVPWYARSIQKIRLARYDLEKGIPIRGSGGLCS
                     ETANGEIGEAVGLIDPNDPRSRFDGYTNSAETQKKILKDAFVKGDAWFRTGDLMRRDA
                     HDYFYFVDRTGDTFRWKGENVATSEVAEALSVFPGVLEANVYGVEVPGGEGKAGMAAL
                     VTGPDFDLAGLAAYLERNLPFYARPIFLRFQQEIEATSTFKQRKIELQKQGFDPATVS
                     DPLYVRDAATGGYLRLTPELYRGIAEAKLKL"
     gene            8576..8797
                     /locus_tag="VGM72_02435"
     CDS             8576..8797
                     /locus_tag="VGM72_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEY4264148.1"
                     /translation="MSETLDLTGLKCPMPALLARRYLMRAPAGAKVEVLTDDPMGVID
                     VPHMCRSEGFELLDIQREGSVARLTLRKP"
     gene            complement(8836..9468)
                     /locus_tag="VGM72_02440"
     CDS             complement(8836..9468)
                     /locus_tag="VGM72_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264149.1"
                     /translation="MWRPVLFLGGAFLAVAALTLATVLGTAGLAGLFGAGPEAKAIAP
                     GFVGVANFGEWRLICVPGPVQLDSLSTTSAPDPAVPAKTHANACRINQEMMAPQQNPR
                     TGEKPGQVIVATNLSLIGLKQTPAAMLRLPVTARPGDMVGFRFDDGAMAQTKVRDCAA
                     TECLAAWTLSGSDWRHLSAARSLQVIFPATGRQWCCSIFLYKDFPRPWPP"
     gene            9567..10268
                     /locus_tag="VGM72_02445"
     CDS             9567..10268
                     /locus_tag="VGM72_02445"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY4264150.1"
                     /translation="MATRRTTVPPAKTSRHTEPAEIPGGTARWRRRKDERPQEILDAA
                     LAVFAEKGFAAARMDAIAARARVSKGTIYLYFPGKEAIFRALVREMLAPELARFSELA
                     AAHRGPVAPFLADILRALGHLIVTSNRVVLPKMVIAEAGNFPDLARIYREEVIERGLA
                     LFGGVLHTGMARGEFRELSIPHAVRLCIAPLLLAAIWRTTFSRLDTVPYDYEGLFEAH
                     IATLLRGLAREEPPQ"
     gene            10383..10493
                     /locus_tag="VGM72_02450"
                     /pseudo
     CDS             10383..10493
                     /locus_tag="VGM72_02450"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
     gene            10608..11210
                     /locus_tag="VGM72_02455"
     CDS             10608..11210
                     /locus_tag="VGM72_02455"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY4264151.1"
                     /translation="MRQQELVRIGGSPRRGLETAQAAYDSARAQRTQAVALQTQAAAA
                     RRQAEAQARQGEANLTTAKFNLSERSVQALVSGQVQDIYFRQGEYANAGTPVVALLPP
                     ENVFVRFFVPEPEVAKLMLGNQVHIRCDGCDGDLIGTISFIASQAEFTPPVIYSVGNR
                     ERLVFKAEARVTNGLPLRPGLPLEVWPVEIAAATAPNPQR"
     gene            11220..>11830
                     /locus_tag="VGM72_02460"
     CDS             11220..>11830
                     /locus_tag="VGM72_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4264152.1"
                     /translation="MSALAIDVYGLTKRFGKKIAVDHVDIAVPEGEVWGFLGPNGSGK
                     TTTIRMLCGLLKPDAGTGKCLGHELLRESESIKREIGYMTQRFSFWEDLSIRENLEFV
                     ARLYEIPRRREKVDATLEQLGLTARQNQLAGELSGGWKQRLALAACMLHEPRLLLLDE
                     PTAGVDPQARREFWEQIHALSQSGLTVLVSTHYMDEAERCDRIV"
BASE COUNT         2486 a         3655 c         3542 g         2147 t
ORIGIN      
        1 tcaccccatt tgatcgaccg ctattccgaa gcgctggctt ggctagcttt ggacgacccc
       61 caacttgaca ggatcagacg cgaactctta aacctcgcag cctcgggcaa aagccttgac
      121 aaagcggccg ttgagaacca cctcgtccgc cagggattag gcgtacttgc agaacggctt
      181 aagacacatt ccgtcctcca gtccgacctc aggggccaag cggatgaaga ggctcgtgaa
      241 gtattgtggc tgcggaccag ggctcaattg gccgaccccg atatttcagg cgcaggtgat
      301 ctcaaggcac gacgcgattc cgcacttcag cgttatctgg acggtggagc caatcaggac
      361 tgggatgagt tgcagcgttt gaacggcgag attcgcaagg ctgtcgaagc cgacgggaaa
      421 cgggatgtga agtgagcaag aaaacccctt cgaaagcgaa accggtcctc aagcccacga
      481 aagtgaagca cgaggccgcc aaacccgcgg tcaagcaacg cgaacccgcc cgtgccgccg
      541 ccgcgaaaac agccataaaa aaagccccct ccaagtcaaa ggtcgaagtg aaaaagcccg
      601 aaatcaagaa gccgatcgcc aagcagtccg ggcccgagcc cagactggcc gccaagcttg
      661 ccgccgccaa accgccggcc aaggaaatcc ccaaggccgc cgcggtgaaa atccccgaac
      721 ccaaatccgc gaaagccgag cccgccaagc cggcaggccc aaagcccgcc cttccggtcg
      781 aactcaaagg ccatcccgcc cagaaactcg atcccaaatc cgccgccaag aagaacggca
      841 cagcgcccga aggcgcgcaa gccccggcac ccgacaaggg cgacccggac gcgccgctcc
      901 tggatttgtc cgacgccgcg gtccgcaaga tgatcacgcg cgccaagcag cgcggctatg
      961 tcacctatga cgagctgaac cgcgttctgc cgtcggacaa agtgtcctcc gagcagatcg
     1021 aggacaccat ggcgatgctc agcgaaatgg gcatcaacgt catcgaaagc gaagagcagg
     1081 aagaggccga gggcgaacag cttcccgccg tccaggccgg acgcgcggtg gccgaagtcc
     1141 ccgaaaaagc cgaagagacc tatgaccgca cggacgatcc cgtgcgcatg tacctgcgcg
     1201 agatgggctc ggtcgagctc ttgtcgcgcg agggcgaaat cgccatcgcc aagcgcatcg
     1261 aggccggacg cgagctgatg atcggggctt tgtgcgaagg cccgctgact ttcgaagccc
     1321 tgaccgtgtg gcgcgacgag ttgatggaag gcaaggcgct gttgcgcgac atcatcgatc
     1381 tcgaagccat gtatggcggc gttccgggcg cggaaggcgc gctcggccag ggcattgccc
     1441 cgcctggcgc acccggtatg cctggcgtgc cgcctggacc tggtgttccc ggggtcccgg
     1501 gcgcggttcc tccagcgcca cccccggtca tgaaggcgcc tgcgccccca ccgccccagc
     1561 ccgtcgctgc aaaaccggcg ccgtcgcgca tgggacccgc cgatgacatc accggcggcg
     1621 atggcggagc cacgccagaa ggcggcggcg aaggcggcgg cgatgaggat ttcgacgacg
     1681 agagcaatct gtcgctctcc gcgatggaag cggccctcaa gccccaagtg ctggcgacac
     1741 tcgaccagat cgcagcgctc tacaaaaagc tcggcaaatt gcaggacgcg caggtcgaag
     1801 ccgcgctcgg cgccgaggaa ttctccaccg ggcaggagcg gcgttttgtc aagctgcgca
     1861 acgagacggt ggaactcgtc aaaagcctgc gcctcaacaa caatcgcatc gaagcgctgg
     1921 tcgatcagat gtacggcatc aaccggcggc tgctctcgct ggaagggcgc ctgttgcgcc
     1981 ttgccgaggc ccatggcgtc gaccgcaccg atttcctcaa gcaatatttc accaatgagc
     2041 tggaccccaa ttgggtgcgg cgcgtcggac gtctgaccgg caccggctgg aaggatttcg
     2101 cgcatgagga gcgcgacaag atcaaggatc tgcgcgacac catccaggcg ctcgcgcagg
     2161 agacgcgcca gtcgatttcc gattaccgcc gcatcgtgca gacggttcag aagggcgagc
     2221 gcgaaagccg tcaggcaaag aaggaaatga tcgaggcgaa tctgcgtctc gtgatctcga
     2281 tcgccaagaa atataccaat cgcggcctgc aattcctcga tctgatccag gaaggcaata
     2341 tcggattgat gaaggcggtg gacaaattcg aatatcgccg cggctacaaa ttctcgactt
     2401 acgcgacgtg gtggatccgc caagcgatca cgcgctcgat tgcggaccag gcgcgcacca
     2461 tccgcatccc ggtgcatatg atcgagacca tcaacaagct tgtgcgcaca tcccgccaga
     2521 tgctgcacga gatcggccgc gagccgacgc ccgaagaatt ggccgagcgt ctcgccatgc
     2581 cgctcgaaaa agtgcgcaag gtgctcaaga tcgccaagga gccgatttcc ctcgaaacgc
     2641 cgatcggcga tgaggaagac tcgcacttag gcgatttcat cgaggacaag aacgcgatcc
     2701 tgcctatcga cgcggcgatc caatccaatt tgcgcgagac caccacgcgc gtgttggcga
     2761 cactgacccc gcgcgaggag cgcgtgttgc gcatgcgctt cggcatcggc atgaacaccg
     2821 atcacacgct ggaagaagtc ggccagcaat tcagcgtgac gcgcgaacgt atccgccaga
     2881 tcgaagccaa agcgctgcgg aaactgaagc accccagccg cagccgcaaa ctgcgcagct
     2941 tcctggataa ttagcccttt cttctcccct tgcgggagag gaagtgcgcc cgcgcactca
     3001 gtacgccgcc ggcgcgccgg gtgaggggga acgaaagaag ccgggggccg atgccgacga
     3061 tcgcttattt ctatggaatc gcgatccgca tgtattttga ggatcacgat cctccgcatt
     3121 ttcacgcgat ttatgcaggc aacgaagctt ttgtgagcat cgcgacaggc gaaatctttg
     3181 aagggcggtt gcccaagaat gccgcgcgtc tggtcaagga atggacactg gcgcgccaat
     3241 cgctcctgat ggataattgg ttgaatgccc gttcaggcaa gccagtgaag aaggttccag
     3301 gcctcgatgc tgaccaaagt gactagagtg gaagcaatcg gagatttcca tcttcgcacc
     3361 catttcagcg acggaagttg cggcgagcat gatttttccg ctcttgcaca caagcccggg
     3421 gaaatgctca tgccgctgcg cgatccttcc tatttcgccc gcgtatttct cgaagacggc
     3481 gcgccgactt ggccgaacgg ttacgacatc gcgccggaat ggctgcgccg cgagatggaa
     3541 gcagccggtg aattgcatcg cgccgctgca gagtgagact tctgcaccca gcagaccgaa
     3601 gcgaaagcgc tgcgggaact caagcgctca cgcaaactgc gctcattctt ggataattag
     3661 taaagcctca cgcgtctaga catattccta ccgtcgatta cgccgcgcgc gggatgcggc
     3721 ggactttgat gccccgttcg ctcttgcgcg cctccgcaag atcgtaatcg agttgcagcc
     3781 cgagccagat ttccgcaccc ccaccgaacg ccttgtccaa ccggattgcc atctccggcg
     3841 agatgccgct ttttccattg accagattgt tgagcgcctg acgggtcacg ccgagcgctt
     3901 tcgcggcttc ggtgacggtc agattcaatg gctcaaggca atcgtgccgc accgataatc
     3961 cgggatgaag aggatgcttc atattcatgg cgcgctcctt cagtgataat cgagataatc
     4021 cacgtcagtg atttcgccgc cctcaaagcg gaaaacgatc cgccaattcg ccagcccggc
     4081 aaatctagat gttctggctt ggacgcccgg tcaaggcgcg ccagaacccg cttgatcttc
     4141 tcgacatgac cggattgagc cctcgtgggc tgtcctcctc gtagagtttt ttcaacccgc
     4201 gatggcgaat tcgccggatc atatttcaaa cgtaatgtga caagcatcac ttgtcaaatc
     4261 tggaatctac ccataactcg tattagaatg gtttgaccaa ggtgcgaaac cgcgtagcga
     4321 tgggaaagga ttgcgcttta tagtggcacg gtctttcgaa agtctccctc ggtgaaaccc
     4381 accatcgcgc gcgttgcgtt gttcgtcgcc ggttttgcgg tgtgggcggc cttttcgatt
     4441 gtgcccggat cggacggagg tttcacgact cgcgaagcgt gggatagcgc gccctattgg
     4501 gaattcggcg tgccgcttct tgccgccgcg caagcgatct cggcagcggc ctcacgcgag
     4561 aagctgctgc aattgccggt ctggcctctt gcgggacatt tcctcggcat gtttgccatc
     4621 caccgcgcgg gggcggattt cgccctggtt ccgctcgcct tcgtgttcat cggcgttccc
     4681 ggctatttca cgctgttggc ggtttcggtc gcggcgcgca aattcgccgg acgcttcgcg
     4741 taacagcgac tcgctgccgt tgaagcgggc aaacgcgcgc cccatgtctt tgggttgaaa
     4801 aacgaaccgg cgcgctttgc tcgatggcac ccaattgcac ctctattcgc accaaataaa
     4861 atggcgtgcg cgagaagatt tccgcacgac gccggcgttt ttcgcgccta ccaccctgaa
     4921 aacagggggg agggggtctg ggttgatttg ggctagcgac ttactctcgc catttgatgg
     4981 agcagcccat cgaggcgatc tgatccttcg gcccgtgacc ggtttcggcg attcccttca
     5041 tcgcctcgta cagatcgcgg cgggcgttcc taaccaaact cgtcttggat tcatccagac
     5101 ggccccgata ttgcaattcg tcctttgcgt tgaagccaaa gaaatccggc gtgcacaccg
     5161 cgccataggc gcgcgccaca tcttgggtcc gatccatgac atagggaaag gtgaaatcat
     5221 gttcgcgcgc gaacagcgtc atcgcgtcga aggaatcgcc cagcgaaatc ttcacatcgt
     5281 tgggcataat cgcgatcacg ccgatgccga gcgctttgag tgcgtgcgtc tcctccacga
     5341 agcggccgac gatcgcgcgc acataggggc aatgattgca gatgaagacg gcgagcgtgc
     5401 cattcggccc gcgcgcatcg ttcagactgt gcgtcttgcc atcgacaccg gggagcgaaa
     5461 aatctttcgc cttccagccg aattcgcaaa tcgggttttg taccagcgcc atcgaagagc
     5521 ctcatcgtcg catttcgcat cgcgcgagcc tgccacctct ttcgctcccg catcaaccgt
     5581 aacctatgcg gggtcgcgcg attcgagcca cgcggtcgcg tccttgcgat tggtgaagct
     5641 ctgatgatgc gtcttggcaa acaggacttg cagaaccttg atgaggggcg cgaactgatc
     5701 gttgcgcacc acataggcga cacgcgaatc gccgccttcg ccccaatcgg ccatcgcgcc
     5761 gatcgcgtgg atcgcgtccc aatccaccga gccggtgaac cccgtcacat ccgccagtgc
     5821 gcgcatgttc gggcacagga tttttctttc caggccttca cgaaaacagg cgatgagaca
     5881 gccgccggcg ggttcctgtt cgataatgac gtgaagccgg tttccggcgg ctttcaacct
     5941 gatccggccc gaagtcccgc ctttttcgat gatcgtgctg tctttggtcg cgtgttcaaa
     6001 atcggaataa aaatccgtgc tgcgttccgc cacaaaaatc cccatgctgc atggcagatc
     6061 ggcggcgttc ccccgtaccg cgtgatcgaa tcatgccggc atggtatgcg ccacgcatac
     6121 cggaccacaa cgcgaaagca tcgaaaatca tgtcattttc gtagggttcg ccaatgttcg
     6181 ttttcagacg ccaatggtcg ttttcagacg taggggaact tcgactgtgc gctggcgttt
     6241 gcagcaatgc tggtggagcg gcgaattcgt gaggagacgc atcatggcca aagcatcgat
     6301 gggacaacgc aagaccgaag gacgcatcat gcatgaattc aagcatggcg aactcaaaag
     6361 cggcaaaagc ggcaagggcg gcaaagtgaa aagccgcaaa caggcgatcg cgatcgctct
     6421 gcacgaggcg ggcgcctcca aatatgatag ccccgcaaaa cgcaaacaga accttgccaa
     6481 aaccaaaggc aaggaagcca aaggcgagac cgctcagcag gaacgcgaag gcaagtcgcg
     6541 cgtcggcgcc cgcggaaagc gcgaaaatac ctctgcgatg gggggcaaga acgcgaagac
     6601 cccgacacgg cgcgcgaagt caaaagggcg cgcaaccgtt tcgcgccccg gcacggcacg
     6661 caagatctca cgcgcctccg gcagaaagcg aagcagcgcc tgattacaat ttgagcttcg
     6721 cctccgcgat gccgcggtaa agctcagggg tcagcctcag atagcctccg gtcgccgcgt
     6781 cgcgcacgta aagaggatcg gaaacggttg cgggatcgaa accctgtttc tgcaattcga
     6841 tcttgcgctg cttgaacgtg ctggtggctt cgatctcctg ctggaaacgc aggaagatcg
     6901 gccgcgcgta gaaaggcagg ttgcgttcca gatacgccgc aaggccggcc agatcgaaat
     6961 ccgggcccgt gacaagcgcg gccattccag ccttgccctc gcctcccggc acttccacgc
     7021 catagacatt cgcctccaag acgcccggaa agaccgaaag cgcctcggcg acctcggagg
     7081 ttgcaacatt ctcccccttc caccggaacg tatcgccggt gcggtcgacg aagtaaaaat
     7141 agtcgtgcgc gtcgcgccgc attaaatcgc cggtgcggaa ccatgcatcg cccttgacga
     7201 aagcgtcctt cagaattttc ttctgggtct cagcgctatt ggtatagccg tcgaaacgcg
     7261 agcgcggatc gttgggatcg atgaggccga ccgcctcacc gatctcgccg ttggcggttt
     7321 cgctgcatag ccctccgctt ccccggatcg ggatgccctt ttcaaggtca taacgcgcga
     7381 gcctgatctt ctggatgctg cgcgcatacc agggcacacg gccgatggcg ccgaccttgc
     7441 cgtcataatt aatcatgccg acattgcctt cggtcgcgcc atagaactcc atgatgcgcg
     7501 ggattttgaa ccgcgcctga aatttgggcc agatctccgg gcgcaggccg ttgccaatgg
     7561 cgatccgcaa acgatgatcg cgctcgtatg gatgttcggg cgtgttcaga agatagcggc
     7621 agagctcacc gatatattgg aacaaagtgg gccgatagcg atggcaatcg tcccagaatt
     7681 ccgtcgccga aaagtggctg cgtaccacca gcgaaccgcc gccgagaaaa gacggaccaa
     7741 gcgcggcgat gccgccagcc gagtgataaa gcggaagaac gtcgtacatg cggtcatcgg
     7801 ggccggcatt caccgcgccc tgaaacgcgc tcatgatgtg gagcagccgc atatggctga
     7861 tattcgccgc cttgggcaga cctgttgtgc ccgaggtgta gatatagagt gccttgtcgc
     7921 ggcagaagat atcgctgcgg cttcctgccg acatgggggc atcggattgc gaactcagga
     7981 tcgcgtcgag attctcacag cctgcccgtg cgccacctgt cgtccagagt accggacgcg
     8041 ggtccaggcc ggcaagcgcg gtggcatagt ttgccgccag ctcttcgccc aggatcaggt
     8101 gcttggcgcc cgcgatctgg atgcaatgcg ccaggggctg gccggtcaga tgggtgttga
     8161 tgagagccgc gatcattccg cttttgatga ggccgagcca cgccatgaca tattcggggc
     8221 ggttctccat cagaagcgag accacatcgc ctttgcgcaa accaatcgcc aatgcccagc
     8281 gggcgaaacg attggcgcca gcatctagcg cttcccagct cacgacgcgg tcttcgtaaa
     8341 ggatcgcggg actggcggga cgctcgcgtg cccagcgttc ggtccaatcg gcaatgctac
     8401 gccgcgcgtc gggacgaacg ccgcgcgtca gcatcagcgt gcgcgccacg gccgcgatat
     8461 aggcggcttc gcggttcacc gcctcggata accccatcgg cgaatcgacc ccgctgacgt
     8521 aaagctgaaa gagagaatag gttgacgcgt ttgacggtca agcaatatgg aaaacatgtc
     8581 ggaaacgctc gatcttaccg gcttgaaatg tccgatgccc gcgcttctcg cgcggcgcta
     8641 tctgatgcgg gcacccgcag gcgcgaaggt ggaggtgctc accgacgatc cgatgggcgt
     8701 catcgatgtg ccccatatgt gccgcagcga aggcttcgag cttctcgata ttcaacgtga
     8761 ggggagtgtt gcgcggttga cgctgcgaaa gccgtgagcc ggcagctcag tcggtccgtg
     8821 aaatttcggc ccgtttcagg gcggccatgg ccgcggaaag tccttgtaca ggaagatcga
     8881 gcagcaccat tggcggcccg tcgccggaaa gatgacctgc aggctcctgg ccgcggaaag
     8941 atgccgccaa tctgaaccgc tcagcgtcca tgccgccagg cactcggtcg cggcacaatc
     9001 ccgcaccttg gtttgcgcca ttgcgccatc gtcgaaacga aagcccacca tgtcgcccgg
     9061 ccgcgcggtc acggggagcc gcagcatggc ggccggggtt tgcttgagac cgatgaggct
     9121 gagattggtc gcgacgatca cctgtcccgg tttttccccc gtccgtggat tttgctgcgg
     9181 tgccatcatt tcctgattga tgcggcaggc attggcatga gttttcgccg gcacggcagg
     9241 gtcaggcgcg gacgtcgtgc tcaaactgtc cagctggacc gggccgggga cgcaaatcag
     9301 ccgccactcg ccgaaattcg caacccccac aaagcctggc gcgatagcct tcgcctccgg
     9361 gcccgcgcca aacagcccag cgagccctgc ggtccctaac acggtggcaa gcgtaagagc
     9421 cgcgacggcg aggaacgcac caccgagaaa aaggaccggg cgccacaaat ttcgctctcc
     9481 catcggcgcc aatcataggc cgactgcttg cgccacttca cgtaaaaata tataaatgac
     9541 tagtcagtta tttatttgat atgagaatgg ccacgcgccg aacgacagtg cctcccgcca
     9601 agaccagccg gcacacagag ccggcggaga taccgggcgg gacggcacgt tggcgcagac
     9661 gcaaggatga acgaccccag gaaatcctag atgccgctct tgcggtcttc gctgagaagg
     9721 gcttcgcggc ggcgcgcatg gatgccatcg ccgcgcgcgc gcgcgtctcc aagggaacca
     9781 tctatcttta tttccctggc aaggaagcga tcttcagagc gctcgtccgg gaaatgctgg
     9841 cgcctgagct tgcgcggttt tccgagctgg cagcggcgca tcggggacct gtcgcgccgt
     9901 tcctggcgga cattctgcgc gcgcttggcc acctcattgt caccagcaat cgcgtggtgc
     9961 tgccaaaaat ggtgatcgcg gaagccggta attttccgga cttggcccgg atctaccgcg
    10021 aagaagtcat cgagcgcgga ttggcgctgt tcggtggtgt cttgcacaca ggcatggcgc
    10081 gcggcgaatt ccgggaactc tcgattccgc atgcggtgcg tctgtgtatt gcccctctgc
    10141 tgcttgctgc gatctggcgc accaccttct cacgcctgga tactgtccct tacgactatg
    10201 aggggctgtt cgaggcgcat atcgcaacat tgctgcgcgg tctcgcgcgc gaggagcctc
    10261 cgcaatgaaa cggtcatcgc ttttgctcgc tttgctttgt ctcgcctcct gcgaagctcc
    10321 tccaaacgat cattggcttg gctatgtcga gggcgagatg gcgctgatcg gcccccccgc
    10381 aacccggttg gatcaccagc ctcgacattg cgcgcggcag tcaggtgaag gccggcgacg
    10441 cgcttttcac gctggatgcc gtgcgcgagc ttgcagcgcg cgacaacgcc actgcgcaga
    10501 tcgcgggggc gaaagagcag gcgagtcagg cggccgcgca gatcggacag gctgaagctc
    10561 agcagacaca gatcgaagcc gatatcgtca agagccagaa agagcttatg cgccagcagg
    10621 aactggttcg catcggcggt tctccgcggc gcggtctgga aacagctcag gccgcttacg
    10681 acagcgcgcg cgcccagcgc acgcaagccg tcgccttgca gacccaggcc gccgccgccc
    10741 gccgccaggc cgaggcccaa gcgcgccagg gcgaagcaaa cctcaccacg gccaaattca
    10801 atctctcgga gcgcagcgtg caggcgctgg tctccggtca ggtgcaggat atttatttcc
    10861 gtcaaggtga atatgccaat gcaggcacgc ccgtcgtcgc cttgctgccg ccggaaaatg
    10921 ttttcgtgcg cttcttcgtg ccggagccgg aagtcgccaa attgatgctc ggaaaccagg
    10981 tgcacatccg ttgcgacggt tgcgacgggg acctgatcgg caccatcagc ttcatcgcct
    11041 cgcaagcgga attcacgccg ccggtgatct acagcgtggg aaatcgcgag cgcctggtgt
    11101 tcaaggccga agcgcgcgtg acgaacggtc ttccccttcg tcccgggcta ccgctcgaag
    11161 tctggccggt ggagatcgct gcggccacgg caccaaaccc gcaacgctga gtttttacga
    11221 tgagcgcgct cgccatagac gtatacggac tgaccaagcg tttcggcaag aagattgcgg
    11281 tcgaccatgt cgacattgcc gtgcccgaag gcgaggtatg gggctttctc ggaccgaacg
    11341 gcagcggcaa gacgacgacc atccgcatgc tctgcggatt gctcaaaccc gatgcgggta
    11401 cgggcaaatg cctcggccac gaattgctgc gcgaatccga aagcatcaag cgcgagatcg
    11461 gctacatgac ccagcgcttc agcttctggg aggatttgtc catccgcgag aatctggaat
    11521 tcgtcgcgcg gctctatgag attccacggc gcagggaaaa agtggacgct acacttgaac
    11581 agcttgggct gacggcgcgg cagaaccagc tcgcgggcga attgtcgggc ggctggaaac
    11641 agcgcctcgc ccttgccgcc tgcatgctgc atgagcctcg cctgctgctg ctcgacgagc
    11701 ccacggctgg cgtcgatccg caggcacgac gggaattctg ggagcagatc catgccctca
    11761 gtcagagcgg cctcacggtc cttgtcagca cccattacat ggacgaagcc gagcgctgcg
    11821 accgcatcgt
//
LOCUS       DASYEG010000064         2270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2193555, whole genome shotgun sequence.
ACCESSION   DASYEG010000064 DASYEG010000000
VERSION     DASYEG010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2193555"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1204)
                     /locus_tag="VGM72_02465"
     CDS             complement(134..1204)
                     /locus_tag="VGM72_02465"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4264153.1"
                     /translation="MREPVAIIGGGLSGLHAARLLHRAQMPFVLLEARTRLGGRILSE
                     DGFDLGPAWFWPDMQPAIAALIEELGLTGFQQYSEGDVLVEQAPNTPPARYPGFGQMA
                     SSFRIAGGTAALVTALARDLPQACIRLGSTVTAAALHEGGVRLTVRNAENESEIVEAR
                     AVLFALPPRLLEKNVAFTPALAPAFGARWRAMPTWMAPHAKFVAVYAKPFWRESGLSG
                     TAQSGVGPMVEIHDASAMPEREDGAALFGFVGVPADARRRVGESVLRQHCLAQLVRLF
                     GADAGKPVSTHFKDWAADGFTATDADVGEHPEVTAAPWFDAAWSHFAVMAGSEAAPAH
                     PGYLEGAVLAAEAAVRAIIASR"
     gene            1333..2076
                     /locus_tag="VGM72_02470"
     CDS             1333..2076
                     /locus_tag="VGM72_02470"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY4264154.1"
                     /translation="MLLKGKTALITGGSSGIGLTTAKLFRENGARVAITGTDAAKLER
                     ARAEIGGDTIAIRADVQSLADLTRMADETKAAFGSLDIFFANAGAAAASPLGGTDEAV
                     YTRLMDINVKGVFFSVQAVVPLMGRGGSIILNSSWLDLRGVAGRGILSASKAAVRSFA
                     RTFAAELAPKGIRVNAVSPGSIVTPLHRGGRSDADFNAYAEMTSKIIPIGRMGQPEDI
                     ANAALYLASELSSYVLGAEIIVDGGRAEL"
     gene            complement(2086..>2270)
                     /locus_tag="VGM72_02475"
     CDS             complement(2086..>2270)
                     /locus_tag="VGM72_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264155.1"
                     /translation="FLRAAVKEACIRRAVIRTPRRIGKLLFEKGTPDFLSHSAYYPQL
                     ESWTTTLMEDQLGALA"
BASE COUNT          423 a          797 c          680 g          370 t
ORIGIN      
        1 tctggcgttg aaagccggga aagaaatctt gcgcctgcgc tgacgccgcc tggctggcca
       61 aggccgccgc gcccgcaccg atcagaaaat cccgccggtt ggcaaacaga tcgctcatgg
      121 tccgcatcct cgatcagcgc gaggcgatga tagcacgcac cgccgcttcc gccgccagta
      181 ccgcgccttc cagataaccg ggatgcgcgg gcgcggcctc gctgcccgcc atgacggcaa
      241 agtgcgacca ggcggcatcg aaccagggcg ctgccgtcac ctcgggatgc tcgccaacat
      301 cggcatccgt ggcggtaaaa ccgtccgccg cccaatcctt gaaatgggtt gaaacaggtt
      361 tgccagcatc cgcgccgaac aggcgcacga gttgggcgag gcaatgctgc cgcagcacgc
      421 tctccccgac gcggcggcgt gcgtccgcag gcacaccgac aaaaccgaac agggccgcac
      481 cgtcttcgcg ttccggcata gcggaggcgt catggatttc caccatcggg ccgacgccgc
      541 tctgcgccgt gccactgaga ccgctctcgc gccagaacgg tttggcgtag acggccacga
      601 atttggcatg cggcgccatc caggtcggca tcgcccgcca gcgcgcacca aatgcgggcg
      661 caagcgcggg cgtgaaagca acgttctttt ccaacagccg tggcggcagc gcgaacagaa
      721 ccgcgcgcgc ctccacgatc tcgctttcat tctcggcgtt gcgcaccgtc aggcgcacgc
      781 cgccctcgtg caacgccgct gccgtcaccg tcgaacccaa ccggatgcaa gcttgcggca
      841 gatcgcgcgc caatgccgtc accagcgcgg cagtgccgcc cgcgatgcgg aaggacgatg
      901 ccatctggcc gaagcctgga tagcgcgcgg ggggcgtgtt cggcgcctgc tccaccagaa
      961 catcgccttc ggaatattgt tgaaagcccg tcaggcccaa ttcttcgatc agcgccgcaa
     1021 tggcaggctg catatcgggc cagaaccaag cgggccccag atcgaaaccg tcttccgaca
     1081 gaatgcgccc gcccaatcgc gtccgcgctt ccagcaggac gaacggcatt tgcgccctat
     1141 gcagcagccg cgccgcatgg agcccgctca atccgccgcc gataatcgca accggttcgc
     1201 gcaatgaaag cccattcccc tattaaggat ttcacgccgc gcatgccgcg tgccgctgtc
     1261 atgcgggtag cggcaatcgc ctgccccgtc tagtctcgcc ctcaaagaca atacgcaatc
     1321 gggagacgaa gcatgttgct caaaggaaag acggcgctga tcacgggtgg ttccagcggc
     1381 atcggcctca ccaccgccaa gctgttccgc gaaaacggcg cgcgcgtcgc catcaccggc
     1441 accgacgccg ccaagctgga acgcgcgcgg gccgagatcg gcggcgacac catcgccatc
     1501 cgcgccgatg tgcagtcgct cgccgacctc acccgcatgg ccgacgaaac caaggccgcc
     1561 ttcggttctc tcgacatctt cttcgccaat gcgggcgccg ccgcggcctc gccgctcggc
     1621 ggcaccgacg aggctgtcta tacccggctg atggacatca atgtgaaagg cgtcttcttc
     1681 tccgtgcaag cagtggtgcc gctgatgggg cgcggcggct ccatcattct caactcctcc
     1741 tggctggacc tgcgcggcgt ggccggacgc ggcattcttt ccgcctcgaa agcggcggtg
     1801 cgttccttcg cgcgcacctt cgccgccgaa ctggcgccca aaggtatccg cgtcaatgcc
     1861 gtgagccctg gctccatcgt caccccgctc catcgcggcg ggcggagcga tgcggatttc
     1921 aacgcctatg cggagatgac atcgaagatc attcccatcg gccgcatggg ccagccggag
     1981 gatatcgcta acgcggcgct ctatctcgcc tccgagcttt ccagctatgt gctgggggcg
     2041 gaaatcatcg tggatggcgg ccgcgccgaa ctctgacctt ggccgtcagg ccagcgcgcc
     2101 gagctgatct tccatcagcg tggtggtcca ggattcgagc tgcggataat aggcgctgtg
     2161 agaaagaaag tccggcgtcc ctttttcaaa taacaatttc ccaatgcgcc gcggcgttcg
     2221 tataaccgcg cgccggatac atgcttcctt aaccgcggcc cgcaaaaacg
//
LOCUS       DASYEG010000065         5336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1325412, whole genome shotgun sequence.
ACCESSION   DASYEG010000065 DASYEG010000000
VERSION     DASYEG010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325412"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..560
                     /locus_tag="VGM72_02480"
     CDS             162..560
                     /locus_tag="VGM72_02480"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY4264156.1"
                     /translation="MRRIRVAIAGILIALATRALAADAPPAHQDIQIVLSNFSFMPNI
                     LHLQRNIPTTLHLMNSASGGHAFSAPELFAAVTVAPEDKGKIMAGKIEVPAGQIVDIT
                     VTPTNLGTYPIVCTHFLHQSFGMRGQATIE"
     gene            complement(580..1359)
                     /locus_tag="VGM72_02485"
     CDS             complement(580..1359)
                     /locus_tag="VGM72_02485"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY4264157.1"
                     /translation="MNIRMLGAFLLVSALTVEAGTAQPVKGETVKIYAAAVMKAPLTA
                     IAADYEKASGNKVTLIFDTAGATEQRFHADPDAVLLITNPVLIQRAESAGVLRDGTTS
                     LLGSTVAGVAVAPGSKKPDVSSPEKLKAALLAAKRIAVSDPARGATVGTHFMKVIEAL
                     GVKDELLPKIIFANDGIETVHLVLDKKVDLGVSQSSEILQGDPEAMAGPFPTEFALVT
                     DFSLWHRNDIPSAAGDFVALLTGPAGRGKLTADGVMPPATR"
     gene            1592..2503
                     /locus_tag="VGM72_02490"
     CDS             1592..2503
                     /locus_tag="VGM72_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264158.1"
                     /translation="MRAARAFASLILTFAAIPALADAGTAIPDLSGTWGRNAFNFEPP
                     PSGPGPIANLRRVGKDASVQTTGGGDPIPLVGDYRNPILKPHAAEVVKRMGEASASGH
                     DILDPSNQCATYSPPYVLAMQQRVTVMQTKNEVTLIYSQDDQVRHVRLNEAHPAHPEP
                     SPMGHSIGHYEGDTLVVDTVGIEVKPHTVVDRFGTPQSEAMHVVERYRLIDAEDARAA
                     QERHEQAAGRLGGKQGNTSFVPGSKKGLQIQATIDDPNIYATQWSGNITYRLTQDPWE
                     ERICAENASLGHPEGIEHPPTADRPDF"
     gene            complement(2522..2995)
                     /gene="smpB"
                     /locus_tag="VGM72_02495"
     CDS             complement(2522..2995)
                     /gene="smpB"
                     /locus_tag="VGM72_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEY4264159.1"
                     /translation="MARKKDADHKVVADNRKARHLYFIESTLEAGIMLMGSEVKSLRG
                     GKGNIAESHAQTKDGEIFLFNSYIPEYTEANRFNHEPRRVRKLLLRKAEARKLSVAVQ
                     REGMTLVPLRVYFNPRGIAKIELGLAKGKKQHDKRQTEKSRDWARDKARLMRAKG"
     gene            complement(2997..3890)
                     /gene="dapA"
                     /locus_tag="VGM72_02500"
     CDS             complement(2997..3890)
                     /gene="dapA"
                     /locus_tag="VGM72_02500"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020055946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEY4264160.1"
                     /translation="MAGIDRIKGSITALITPFRNGALDEDAFCRLVEWQIAEGTHGLV
                     PVGTTGESPTLSHKEHMRVVELCAATARGRVPVIAGAGSNSTREAIELARHAKDVGAN
                     AALSVTPYYNKPTQEGIYRHFASIAEAVDIPIVLYNIPGRSIVEISTETMARLAGIAN
                     IVGVKDATGNLARASRDRSALPKSFIRLSGDDGTALGFMAHGGRGCISVSSNVAPKLC
                     ADFQNACAAGDYAKALTYQDRLMALHDGLFCEASPAPTKFAASLLDLCDPEVRLPIVP
                     LTAQGRETVRAAMAAAGLLNG"
     gene            4062..>5336
                     /locus_tag="VGM72_02505"
     CDS             4062..>5336
                     /locus_tag="VGM72_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264161.1"
                     /translation="MRFARRSPAATGRIVTAVLTLGLAIVPPASNVLAQSEESSVPAA
                     APEAQTPFKAEPSGDSSAVFDAPGMPGVRMMAPIPDRLEPASRSPGIVAAPATKTDGN
                     DRVILSQAQAQQAQAQAQQTQAQQALAQALAAGHDWGRARLLAAQTANPIVKSIVEWR
                     YLLDEMSGASFDSINAFLNAHPNWPRYDALLIRAEKAMPDMDPRDVIGWYVNRQPLSG
                     FGYIRLGSALMDSGKRAEGSALIRKGWIDFTFAPFDEKAIIAAHGDVLGAPEQKARLL
                     KLLGHEDIGGAKRQMQRVDSDTRRLGNALLQIKANPATVKQVLTSFPVSARNAPELLL
                     EAARALRRRNQDEAAWDLMAQAPTDKENMAVAERWSSERQIMARDALKAGKVELAYQF
                     ASTPELGPDSGAAFMDAEFLAGWIALRNLHKPE"
BASE COUNT         1042 a         1686 c         1554 g         1054 t
ORIGIN      
        1 cccattcctc cttggaacga atgagcttgt ccttgatctg gccaagaagg cttggcttac
       61 cggggttgtc gccgttcatg cggatgggta tcttagtcaa tgatggctag agcgccaaat
      121 gtgcgttcca tacctctaca attcacctgc gtggtattgc cgtgagacgc attcgcgttg
      181 ccattgctgg catcctcatc gctctggcaa cccgcgcgct cgcggcagac gcgcctcccg
      241 cccatcagga cattcagatc gtactgagca atttcagctt catgccaaat atcctgcacc
      301 tgcagcggaa tattcccacc acgctgcatc tgatgaacag cgcctcgggc ggacacgcct
      361 tctccgcgcc tgaacttttt gccgcggtca cggtggcgcc cgaggacaag ggaaaaatca
      421 tggccggaaa aatcgaggtg cccgcagggc agattgtgga tatcaccgtc acacccacaa
      481 accttgggac ctatcccatc gtctgtacgc attttttgca tcagtccttc ggaatgcgcg
      541 gccaggcgac gatcgaatag ctttctcttc gtccggtgtt cagcgggtgg cgggaggcat
      601 gactccgtcc gcagtcaatt tgccgcggcc cgccggaccg gtcagcagtg caacgaaatc
      661 accggccgcc gacggtatgt cgttgcgatg ccaaagcgaa aaatccgtga ccaatgcgaa
      721 ttccgtggga aacggtcccg ccatggcctc gggatcaccc tgcaggatct cactggattg
      781 cgatacgccc agatcgactt tcttgtcgag aacgagatgc acggtttcaa tgccgtcatt
      841 ggcaaagatg atcttcggaa ggagttcgtc cttcacgcca agggcttcga tcactttcat
      901 gaaatgcgtg cccactgttg ctccgcgtgc cggatccgaa acggcgattc gtttggcggc
      961 gagtaacgcg gccttcaatt tttccgggct ggagacatcg ggttttttag accctggagc
     1021 caccgccacg cctgcgacgg tactgcccaa gagactagtg gttccatccc gaagcacgcc
     1081 cgcgctttcg gcgcgctgta tcagcaccgg attggtgatc agcaacaccg cgtcgggatc
     1141 ggcatgaaat ctctgctccg tggcgccagc cgtatcgaag atcagggtca ctttgttgcc
     1201 gctcgctttt tcgtaatcgg cggcgatagc ggtaagagga gccttcatca cggccgcagc
     1261 gtagattttc acggtttcgc cctttacagg ttgcgcggtc ccagcctcca cggtcagcgc
     1321 tgaaacaagc aaaaaagcgc ccaacatccg tatattcata cgtgctctcc ctctaaccga
     1381 accgaacagc gtgagcatgc gtcctggcga ggttggggtc caggccaacg atcgagtcgg
     1441 cggcatggcc gacaggaagc gcaacgcgat acagcgacaa aatgcgccat ctgcgtgctt
     1501 tccggccacc gccggcaatt gtattctttc gccagttcgg caacacctcg cccccggcga
     1561 acctcagccg gatttgaaac ggaagatttc gatgcgtgca gcgcgggcgt tcgcctcact
     1621 gatcctgacg tttgccgcca ttcccgctct tgcggatgcc ggtacggcca taccggacct
     1681 ctctggcacg tggggccgca acgcttttaa cttcgaaccg ccaccgtcgg ggccgggacc
     1741 gatcgccaat ctgaggcgcg tcggaaagga tgccagcgtc cagactacgg gcggcggcga
     1801 ccctatcccg ctggtcggcg attacaggaa cccgatactc aagccgcatg ctgcggaagt
     1861 cgtaaagcgc atgggcgaag cttcggcgag cggccacgac atcctcgatc cgtcgaacca
     1921 atgcgcgacc tattcgccac cctatgtcct cgccatgcag cagcgcgtaa cggttatgca
     1981 gacgaagaac gaggtgaccc tcatttatag ccaggacgac caggtccgcc acgtacgctt
     2041 gaacgaagcc catcccgccc atcctgagcc ctcaccgatg ggccattcca ttgggcacta
     2101 tgaaggggac acgcttgtcg tcgacaccgt cggcatcgag gtcaaacctc acaccgtggt
     2161 cgaccgcttc ggtaccccgc aaagcgaagc catgcatgta gtcgagcgct atcgcctgat
     2221 cgatgcggaa gacgcgcgcg cggcccagga acgtcacgaa caagccgcgg gccgccttgg
     2281 cggcaagcaa ggaaacacgt ccttcgtccc cggctctaag aagggcttgc aaattcaggc
     2341 gacgatcgac gacccgaaca tttacgccac gcaatggtcg gggaacatca cctaccggct
     2401 gacgcaggat ccttgggaag aacggatttg cgcagagaac gcatcccttg gccaccccga
     2461 agggatcgag catccgccga ccgcggacag accggacttc tgatcggcga tacgcttgcg
     2521 cctaaccctt ggcgcgcatc aggcgcgcct tgtcccgggc ccagtcgcgg gatttttcgg
     2581 tctggcgctt gtcgtgctgt ttttttcctt tcgccaggcc aagctcgatc ttggcgatgc
     2641 ctctcgggtt gaaatagacc ctgagcggaa cgagcgtcat gccttcacgc tgcacggcca
     2701 cgctgagctt tcgcgcttca gccttgcgca acagcagctt gcgcacgcgg cgcggctcat
     2761 gattgaagcg attggcctct gtgtattcgg ggatgtagga attgaacagg aagatctcgc
     2821 catccttcgt ctgggcgtgg gattccgcga tgtttccttt gccgccgcgc aggctcttca
     2881 cctcgctgcc catcagcatg attcccgctt cgagcgtcga ttcgatgaag tagagatgac
     2941 gcgctttgcg attgtccgcg acgaccttgt gatcggcgtc tttctttctt gccatgtcag
     3001 ccgttcaaca gcccggccgc cgccattgcg gctcgcacgg tctcgcgacc ctgggcggtc
     3061 aacggcacaa tgggaagccg gacttcggga tcgcaaagat ccagcagcga cgcggcaaat
     3121 ttcgtgggcg ccgggctcgc ttcgcagaag agaccgtcat gcagcgccat caaccggtcc
     3181 tgataggtaa gcgccttggc atagtcgccg gcagcacagg cgttctggaa gtccgcgcaa
     3241 agcttgggcg cgacattgga ggagaccgaa atacaaccac gcccgccatg ggccatgaag
     3301 cccagtgccg ttccgtcatc gcccgaaagc cggatgaaac tcttgggcag cgcggagcgg
     3361 tcacggctcg cgcgtgccaa attgcccgtc gcatccttca ctccgacgat attcgcaatc
     3421 ccggcaagac gcgccatcgt ctcagtcgag atctcgacga tgctgcgtcc gggaatgttg
     3481 tagagcacaa tcggaatgtc caccgcctcg gcaatgctgg cgaagtggcg ataaatccct
     3541 tcctgcgtgg gcttgttgta ataaggcgtg accgacagcg cggcattcgc cccgacgtcc
     3601 ttggcgtggc gggccagttc gatggcctcg cgcgtggaat tggaacccgc gcccgcgatg
     3661 acgggtacgc gcccgcgcgc ggtggcggcg caaagctcga ccacacgcat atgttcctta
     3721 tggctcaggg tgggcgattc tcccgtcgtg cccaccggga ccaggccgtg ggttccctcg
     3781 gcgatttgcc actcgactag acggcagaag gcatcctcgt cgagtgcgcc attgcggaac
     3841 ggcgtgatca gcgccgtgat ggaacccttg atgcgatcga tgccggccat atcccaatcc
     3901 cgaaaattag cgcccgactt taaccttcgc gtaagcacgg cgcaaagatt tacctttgcc
     3961 tgcggtcgat tcacaaaatc gggaagagtg tggtcagaca tgcatagcat tgttatgctt
     4021 gtttcattca gctccgttgg cggggattcg acgagttcaa gatgcgattt gcgcgcaggt
     4081 caccggctgc gacagggcgg attgtcaccg ccgtcctgac gctcgggctc gcgatcgttc
     4141 ctccggcttc caacgtgctc gcccagtccg aagaatcgtc ggtgcctgcg gccgcgcccg
     4201 aagcgcagac gcctttcaaa gctgagcctt ccggtgattc gagcgccgtg ttcgatgcgc
     4261 ccggcatgcc cggcgtgcgc atgatggctc cgattccgga caggctggag cctgcctccc
     4321 gctcacccgg tatcgtggcg gcgcccgcca ccaaaacgga cggcaacgac cgcgtgattc
     4381 tttcccaggc ccaggcccag caggcccagg cccaggccca gcagacccag gcgcagcagg
     4441 ctctggccca ggctctggcg gctggccatg attggggccg cgcccgtctt ctggccgccc
     4501 aaaccgcgaa cccgatcgtc aaatcgatcg tggaatggcg ctacctgctc gacgagatga
     4561 gcggcgcgag tttcgattcc atcaatgcgt tcctcaatgc ccatccgaac tggccacggt
     4621 acgacgcgct tcttattcgc gccgaaaagg cgatgccgga tatggacccg cgcgatgtga
     4681 tcggctggta tgtcaatcgc cagcctctct cgggctttgg ctacattcgg ctgggttctg
     4741 ctctgatgga tagcggcaag cgcgccgaag gcagtgccct cattcgcaaa ggttggatcg
     4801 atttcacgtt cgcgccgttc gacgaaaaag cgattatcgc ggcgcatggc gatgttctcg
     4861 gagcccctga gcaaaaagcc cgtctcctaa aactgctcgg gcacgaggac attggcggcg
     4921 ccaaacggca aatgcaacgc gtcgactccg atacgcgccg cttgggcaat gcgttgctgc
     4981 agatcaaagc aaatcccgcg acggtcaaac aggttctgac ttcgtttccc gtttccgcgc
     5041 gaaacgctcc ggaattattg ctcgaggcag cccgtgcgct tcgccgccgc aatcaggatg
     5101 aggcggcttg ggacctgatg gcgcaggcgc ctacagacaa ggagaacatg gcggtcgccg
     5161 agcgctggtc ctccgaacgt cagatcatgg cgcgcgacgc gctgaaggct ggcaaggtcg
     5221 aactcgccta tcaattcgcc tctaccccgg aactcggccc ggattcgggc gccgccttca
     5281 tggacgccga attcctcgcc ggatggatcg cactgcgcaa tctgcacaaa cccgaa
//
LOCUS       DASYEG010000066        14281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1325599, whole genome shotgun sequence.
ACCESSION   DASYEG010000066 DASYEG010000000
VERSION     DASYEG010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 14281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14281
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325599"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1775)
                     /locus_tag="VGM72_02510"
     CDS             complement(138..1775)
                     /locus_tag="VGM72_02510"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY4264162.1"
                     /translation="MSTAKANPRTYPDLHDHIAALEKAGQLIRVTRPINKDTEMHPLV
                     RWQFRGGIPEDERKAFLFTNVIDSKGKHYDIPVIVGGLAANREIYRIGLGCDLDKIEA
                     TWARATAAPIAPRVVTNAPCHEIVITGDELNKEGLGLDGIPVPISTPGWDNAPYTTLS
                     QYITKDPETGVQNMGNYRGQVKTPRRLGMNPSLELRPGIYQHWLKYKKRGEKMPAAVV
                     LGAPPAVTFVSTQKISESLDELDVAGGLVGAPINVVKAVTVDLMVPAEAEIVIEGYID
                     TEWLEPEAPFGESHGHVNLQEYNAFMDVTAITRRKDAVLTSIISQVTPSESSLIKRVS
                     LEPLLLQGLHALGMKFVKRVALHEPLTNLRKLCMLHVEKGTPRQEVWRAMYGLCAQHR
                     AVGKYVIALDEDIDPQNMDAVWWALSYRCNPALDVEILKHRDQGHGPRSLRNDGEDAS
                     VLFDATLKEPFPPISLPKREYMENARKIWEELGLPPLKPQAPWFGTSLGEWDERFDVM
                     AERAVKSDYWVTGDIIAQQRRNDVTMNTEIRRVPKKA"
     gene            complement(1881..3101)
                     /locus_tag="VGM72_02515"
     CDS             complement(1881..3101)
                     /locus_tag="VGM72_02515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4264163.1"
                     /translation="MNTSEDFPNANSLVRMWTAGALASPMPIVHTSKTCSEVFDWFTA
                     NSTQPAAAVVDNENRVIGIANRLRFLARYAQRFAPELYGKCSIMKLANARPLIIDENV
                     SVLDLATTLTLEYPDALRECFVVTRAGRYLGIGTSEALVRCKMEILGARENELKSALL
                     IATDASKTKSHFLALMSHELRTPLNAIIGFSEILAAQMYGPLGADRYREYACDIHGAG
                     RHLLALINDILDLSKAEAGKLDLHLEAIDVATLIADCVRLTSERAREQSVQVAVRAAP
                     DLPALHADALRVKQVILNLLTNAIKFTLAGGDVEVAAEIGREGEFLLSVRDTGIGMAP
                     ESIPVALEPFRQIASPFTRNVEGTGLGLSLVKVLTEQHGGEIAIESAPSQGTMVRLRF
                     PASRTLWPAAAIIA"
     gene            complement(3261..4013)
                     /locus_tag="VGM72_02520"
     CDS             complement(3261..4013)
                     /locus_tag="VGM72_02520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4264164.1"
                     /translation="MKLTGQVAIVTGAGRNIGEETAKLFAKEGAAIAVVDMDAGRADH
                     VTDAITAAGGKAKTYICDVSQEDQIIATVTAVAADWGRVDMLVNNVAVSDNKHLFDIT
                     KAEWDRVIAVCLTAPMLFSKYAAKAMIEGGRGGRIVNVSSTSGHYGRGRAIAYTTAKA
                     GVINLTRSLAIQLAPHNIRVNCVVPNKIGSPVGKDAFNPDRKVLNLRNRPGEPIDLAR
                     AMLFLVSDDSDFIAGTELFVDGGCAAMMPGGD"
     gene            complement(4094..4693)
                     /locus_tag="VGM72_02525"
     CDS             complement(4094..4693)
                     /locus_tag="VGM72_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264165.1"
                     /translation="MLEPKQLAPDGRVASAILDHMRLPAFLVESNADLRYSNAAGIAA
                     LKDGDYFKQAGETLCAMHPNDDVRLRSAITSACASGERRTLMMNDARNQRPNLVTAIP
                     FLDDEQAARKEVIVFVQKCEPANDIFASTLRQLFRLSAAETAIAAALVSGADIERIAE
                     MRNAKITTVRTQIAAMLLKTRTRRQGELIALFSRICSLP"
     gene            complement(4858..5808)
                     /locus_tag="VGM72_02530"
     CDS             complement(4858..5808)
                     /locus_tag="VGM72_02530"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEY4264166.1"
                     /translation="MHDSKRILVTGGAGFIGSFLCEKLVAQGHDVICVDNMYTGSRRN
                     VAKLLPNPRFEFIRHDVTFPLYVEVDEIYNLACPASPIHYQRDPVQTTKTSVHGAINM
                     LGLAKRLKARILQASTSEVYGDPSVHPQPESYWGNVNPIGPRSCYDEGKRCAETLFFD
                     YHRQHRLPIKIARIFNTYGPRMHPNDGRVVSNFIVQALKGEAITLFGDGSQTRCFCYV
                     DDLVDGLIKLMNTPPELTGPVNLGNPTEFSVRELAEKIIRMTGTRSRIEIRPLPTDDP
                     RQRQPDIALARKTLDWTPKIALDEGLTITIAYFRELLELN"
     gene            5896..6300
                     /locus_tag="VGM72_02535"
     CDS             5896..6300
                     /locus_tag="VGM72_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264167.1"
                     /translation="MRIRFDKFVYSAAIGLLAAMGPARAAANDEVPTALMSAAECMVR
                     TLKAMPGVTEVQIRVSAGQGGAYPVLQYRFADAFGGRRFTEVSLFEIVGAADPFVFDK
                     ADIDEDVVAERLLPVWKARCHSGFGYITSQPQ"
     gene            6546..7082
                     /locus_tag="VGM72_02540"
     CDS             6546..7082
                     /locus_tag="VGM72_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02444 family protein"
                     /protein_id="HEY4264168.1"
                     /translation="MTKATSEIERDGSPFWRFSLRFYARAKMSAACLVLQDEAGVDVN
                     LLLFLLFLAEHQRQLGAADVARLDAAVHDWRDSVVKPLRALRRALKTGVGEISVTISE
                     TFRGQIKRLELDSEHIEQSVLERFNAAELGAPAVSRMAAAETNIASYEIHLGRFPGEA
                     KANILSGFAEFGPHHPAN"
     gene            7236..8699
                     /locus_tag="VGM72_02545"
     CDS             7236..8699
                     /locus_tag="VGM72_02545"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4264169.1"
                     /translation="MGNSDLIVGEAVPPRTMREQLKNPARKKSAARVAPDERAKETAS
                     DTKDAPTDVIARLERRLAGLEKTIANIANVVERHEITLREREEALASVAQSVVALRSQ
                     MEQSEKQHAAETAELHAALAQAGTRLNALEAAKAATPAPAAAIPGSAIEAAPCAPPIV
                     WAGENGNPAAAAATRAPQVSATGTKDTQVPSYLSAARSAANHGTGVVDKKAAVGARRI
                     GRTQLMVFGCVAPLVLLATGAFVLNHHPVTAEPVPAKTAFVPPPEAPLPPPPQIIIAP
                     PSDPTAEELANSASLDALQQKAKTGDADAERDIGLKYLAGDTIPVNEEEAARWLLRAS
                     YRGEPSAEYWLGTLYSRGRGVPADLPQANHWYGASAKQGNPYAMYRLGIANFEGLGMD
                     SNAEQAAHWFTQAAEMGLIDSQFDLAVLYERGMGVMQSLPDAYKWYTVAATQGDKEAG
                     ERAAQLAKALKPAELADATRASAEFKPELPAAVAAPK"
     gene            8832..9743
                     /locus_tag="VGM72_02550"
     CDS             8832..9743
                     /locus_tag="VGM72_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264170.1"
                     /translation="MRPFLIATSLVALLIVAASGQEQKKTSGAKIPALASSDFGWQTN
                     FEDWQDPPPGYGHGPMKNDPAYPFVNNSVVDAPENDLAKSQDKNFININGRQPTVRIA
                     NSKDPILKPWAAAAVKATNDEILQGKRQVSFVAQSRCWPGGVPGQLIYPFEPLYFIQM
                     PKEVWMIWQRDHMIRRIYLTGKHSADLKPSWFGESIGHYENGDTLVVDTVGLSANKLS
                     YIDNFRTPHSEKLHVVERFRISPDNNTLTAIARAEDSDAYNAPITMMRQWFKVNEPIA
                     ETVCAENNDDHFHQNLYPIPEAENPDF"
     gene            complement(9906..11258)
                     /locus_tag="VGM72_02555"
     CDS             complement(9906..11258)
                     /locus_tag="VGM72_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4264171.1"
                     /translation="MKRTDLLGIAVLLLPLGGCAINPLSTATTPAPMAKAKQATDAKQ
                     PSALPTDLESAIRSAQAARKNGDLAGATKILSQLVLVTPDDAHVLGEYGKALAAEGRS
                     DDALAFLERATQLDPGDWSLYSAEGVALDQKGNYQAAQTFYARALTLKPGEASVLNNS
                     ALSHMQSGDLDGAEQLLRQAMPGSPDDARIAQNLALVQSLKAAQPPKAMAAATPAPAP
                     TATPAPVQMASVTPPASAMPAIPTAVAPLPVPAPVVTTTLETQMPTPAVKPEPSAVEA
                     LKSDPSVRMQALPKETAAAPAPHELPAKPELASVPEKKAVQAPAPKAAATATPAPKSV
                     NASTPAPKVAAAPAAAPAPNNATPAGPAPRLITAAARTPASSSSFYVQAGSFPTEERA
                     VKSASNLDSMGARVMSGTVDGHAVYRVRIGPFLNIRQANAAVDQAHALGHADLAIVTE
                     "
     gene            11486..13156
                     /locus_tag="VGM72_02560"
     CDS             11486..13156
                     /locus_tag="VGM72_02560"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY4264172.1"
                     /translation="MTISVPEAGRTAQQKSKIAKDFQEHLAALEARGLVVRVDRPINK
                     DTELHPLARWQFQGAIPEDKRRAFLFTNVVGSDGKKFDMPVVAGAFAASPEIYALGMG
                     VALEDIGPAWLRAIQNPISPVTVKSGACQEVVIDGDALKTPGGGLAALPVPISTPGFD
                     AAPYLTATICVTKDPETGVRNMGTYRGQLKATDRLGVRMASRLGGAGGYLHWLKYQKL
                     GQPMPVAIVIGCAPVIFFTGPQKLGIDFDEMGVAGALLGEPVKTVRCVTNDLEVPADS
                     EIVIEGLIDSQLLEPEGPFGESHGHVALEDYNMSMQVTAITMKKKPVFVSIISQVTPS
                     ESSVTKVVAYEPLFLAHLRGHLGVKGIKRVVMHEALTNVRPVIFLTFAHGTPKTEIWR
                     GLQGAATLQAQCGKIVIAVGEDIDPTNTDAVFWSLAYRTNLGEDLHVAPYKDAGHGPK
                     SGGARSSDSTLLVDATPKHAMPPFALPAREFMERAKVIWDELGLPELNPRPPWYGYSL
                     GDWTANWDNWAKRAVEGRWEETGAETYAGRKGGMKPETPVRDVKAKEK"
     gene            complement(13159..13944)
                     /locus_tag="VGM72_02565"
     CDS             complement(13159..13944)
                     /locus_tag="VGM72_02565"
                     /inference="COORDINATES: protein motif:HMM:NF018420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HEY4264173.1"
                     /translation="MNRSQGLGWTAALLFAVGLSVTLAQAQPAAPKPPAPTPSRGLIT
                     QPAGQPRVDLNLVLAVDASGSVDDDRFELQKQGYAKAFLNPRVLNAIRNGNEAAIAIS
                     MVQWTGPTLHVIMLPWMVIKDERSAQLAAAAIAASPRQIFGGGTSLSGAIDFSVTMLT
                     ASPYRAERKVIDISGDGSNNLGRPAEQARDEAVKMGVRINGLPILAVEPDLDQYFRES
                     VIGGPGAFIIPVKDYNAFGEAIMRKLVQEISQDQRPRPKFAAK"
BASE COUNT         2747 a         4238 c         4445 g         2851 t
ORIGIN      
        1 cgctcttgcg gctcttcggc atcaacggtt caaggagcgt ccattcctca tcggataaat
       61 caaatcgcat gatccacctc ccatcacggt gaatcactgt tcgtcaaaat tttcaacgac
      121 ctatgtgggt acagacccta ggccttcttc ggtacgcggc ggatttcggt gttcatcgtg
      181 acatcgttgc gcctctgttg cgcgatgatg tcgcccgtca cccaatagtc gcttttgacc
      241 gcgcgctcgg ccatcacgtc gaagcgctcg tcccattcgc cgagcgatgt cccgaaccac
      301 ggcgcctgcg gcttgagcgg aggaagcccc agctcctccc agattttccg tgcattctcc
      361 atatattcgc gcttgggcaa cgatatcggt ggaaacggtt ccttcagcgt cgcgtcgaac
      421 agcacggagg catcctcgcc atcattgcga aggctccgcg ggccgtggcc ctgatcgcga
      481 tgcttcagga tttcgacgtc gagagccgga ttgcaacgat acgaaagcgc ccaccacacc
      541 gcatccatgt tctgcggatc gatatcctcg tccagcgcga tgacatattt tccgacggcg
      601 cgatgctgcg cgcaaagccc atacatcgca cgccagactt cctgacgcgg cgtgcctttt
      661 tcaacgtgca gcatgcacaa cttgcgcaga ttggtcagcg gctcgtgcag cgcgacgcgt
      721 ttgacgaatt tcattccgag cgcgtgcagg ccctggagca gtagcggctc gagcgacacg
      781 cgcttgatca ggctcgactc gctcggcgtc acctggctga tgatagaggt gagtacggca
      841 tccttgcgcc gcgtgatcgc ggtcacatcc atgaaggcat tgtattcctg caggttgacg
      901 tggccatggg attcgccgaa cggggcttcg ggctccagcc attcggtgtc gatatagcct
      961 tcgatgacga tttccgcttc ggcgggcacc atcagatcga cggtcaccgc cttgacgaca
     1021 ttgatcggcg cgccgacaag gccgccggcg acatccagtt cgtcgagcga ttcggaaatc
     1081 ttctgggtcg agacgaatgt caccgcgggc ggcgcaccga ggacaacggc ggcaggcatt
     1141 ttttccccgc gcttcttgta tttcagccaa tgctgataga tgccgggccg aagctccagc
     1201 gacggattca tgcccaatct gcgtggcgtt ttcacctggc cgcggtaatt gcccatgttc
     1261 tggacgccgg tctccggatc cttggtgatg tattgcgaca atgtcgtgta gggcgcgttg
     1321 tcccaacccg gcgtcgagat aggcaccgga attccatcca gccccaggcc ctccttgttc
     1381 agttcatcgc cggtgatcac gatctcatgg cagggcgcgt tcgtgaccac gcgcggcgcg
     1441 atcggcgccg ccgtcgcgcg cgcccatgtc gcttcgattt tgtcgagatc gcaaccgagc
     1501 ccgatgcggt agatctcacg atttgccgcg agcccgccga caatgaccgg aatatcatag
     1561 tgcttgcctt tgctgtcgat cacattggtg aacaggaagg ctttgcgctc gtcttcgggg
     1621 atgccgccgc gaaactgcca gcgcaccagc ggatgcatct ccgtgtcctt gttgatcggc
     1681 ctggtcacgc ggatgagctg gccggctttc tccagcgctg cgatgtgatc gtgcagatcg
     1741 ggataggtgc ggggattggc cttggccgtg ctcatgcgac gcctccttcg actgaggttc
     1801 tcatgcgccg aaggcgcacg cgaaatcaag cccgctttac gaaggattgt tccggcgtct
     1861 cccaaccggc acgctggcgt tcaggcgatg atcgcggcgg ctggccatag ggtgcgcgac
     1921 gcgggaaacc gcaaccggac catcgtgcct tggctgggtg cgctctcgat cgctatttca
     1981 ccgccatgct gctcggtcag caccttcacc agcgacaggc cgaggccggt tccttccacg
     2041 ttgcgggtga agggcgaggc aatctgacgg aatggctcca gagccacggg gatcgactcc
     2101 ggcgccatgc cgatgcccgt gtcccgcacg ctgagcagga attcgccttc gcgtccgatt
     2161 tcggccgcta cctcgacatc gcctccggcc agggtgaact tgatcgcgtt cgtcaggaga
     2221 ttgagaatca cctgcttgac gcgcaaagcg tcggcatgca gtgcgggcag atcgggtgcg
     2281 gcgcgtacgg ccacctgcac gctttgctcc cgcgcgcgct cagaggtcaa gcgtacgcaa
     2341 tcggcgatga gtgtagcgac atcgatggct tccagatgaa ggtcgagctt tcctgcctcg
     2401 gctttggaca gatcgagaat atcgttgatc agcgccagca gatgccgtcc agcgccatga
     2461 atatcgcaag cgtattcgcg atagcggtcc gcgcccagag gtccgtacat ttgagccgcg
     2521 aggatttccg agaaaccgat gatggcattc aatggtgtac gaagctcgtg gctcatgaga
     2581 gcgagaaaat ggctcttggt cttgctcgcg tcggtcgcga tcaagagagc ggatttcagc
     2641 tcgttttcgc gcgctcccag gatctccatc ttgcaccgga ccagggcttc gctggtgcct
     2701 atcccgagat agcgtccggc tcgtgtcacc acaaagcatt cgcgcagcgc gtccggatat
     2761 tccagagtga gagtcgtcgc caaatccaat acggagacgt tttcgtcgat gatcagcggc
     2821 ctggcgttcg ccagtttcat gatcgagcat ttgccgtaaa gttctggcgc gaagcgttgc
     2881 gcatagcgcg ccagaaagcg aagccgattt gcgatgccga tcacgcgatt ttcgttatcg
     2941 accacggcgg cggcgggctg tgtgctgttc gcggtgaacc aatcgaagac ttcggaacat
     3001 gttttgctgg tgtgaacgat cggcatgggc gaagcgagcg cgccggcagt ccacattcgc
     3061 acaagggaat tcgcgttggg aaaatcttcg gatgtattca cgaaatcata tccgcggagg
     3121 gcagaagtgc tcgggcggtt atccaatagt ggcgttatct agcggttaac gatgacgctt
     3181 gttcgcggaa accgcttgct ttttcgcatg aactttttgt ggcggagcta tgaaccgccg
     3241 gcagacgtcg aacgcggcgc tcaatcgcct ccgggcatca tggccgcaca gccaccatcc
     3301 acgaaaagtt cggtgccggc gatgaaatcc gaatcgtccg agaccaggaa cagcatggcg
     3361 cgcgccagat cgatgggttc gcccgggcgg ttgcgcagat tgaggacttt acggtcggga
     3421 ttgaacgcat ccttgccgac cggcgagccg atcttgttcg gcacgacgca gttcacgcgg
     3481 atgttgtgag gcgcaagctg gatcgcgagc gaacgggtga ggttgatcac accggctttg
     3541 gccgtcgtat aggcgatggc ccgcccgcgc ccgtaatgcc ccgaggtcga gctgacattg
     3601 acgatgcggc ctccgcgccc gccttcgatc atggctttcg ccgcatattt gctgaacagc
     3661 atgggggcgg tcaggcagac cgcgatgacg cggtcccact cggccttggt gatgtcgaag
     3721 agatgcttgt tgtccgaaac cgccacattg ttgacgagca tgtcaacccg gccccaatcg
     3781 gccgccacgg ctgtcacggt cgcgatgatc tggtcttcct gcgacacatc gcagatatag
     3841 gttttggcct tgccccccgc cgctgtgatc gcgtccgtga catgatcggc gcggccggcg
     3901 tccatgtcga ccacggcaat ggcggcccct tccttggcga acagcttggc cgtctcttct
     3961 ccgatattgc gtcctgcgcc cgtcacgatg gcgacctgtc cggttaattt catatgcgct
     4021 ctctcgcgtt ttgcaacgcg ctagcgatag ctttcttgga cggcttcagc aatgccggcg
     4081 cggacctgcg gtctcagggc agactgcaaa tccgcgagaa cagcgcaata agctcgcctt
     4141 ggcgccgcgt gcgcgtcttc aacagcattg cggcaatctg tgtgcggacc gtggtgatct
     4201 tggcattgcg catctcggcg atacgctcga tatccgcgcc cgatacgagg gctgccgcga
     4261 tcgcggtctc ggcggctgag aggcggaaca actgccgcag tgtgctcgcg aagatatcgt
     4321 tggccggttc gcacttctgc acgaagacga tgacctcctt tcttgccgcc tgctcgtcgt
     4381 cgagaaacgg aatcgcggtg acgagattcg gccgctgatt gcgcgcgtca ttcatcatga
     4441 gcgtgcggcg ttcgccgctg gcgcaggcac tggtgatcgc gcttcgcaaa cggacatcgt
     4501 cgttcggatg catcgcgcag agcgtctcgc ccgcctgctt gaaatagtct ccgtccttga
     4561 gggcggcgat gcccgcggcg ttgctgtatc taaggtccgc attgctctcc acgaggaaag
     4621 cgggcagccg catatgatcc aaaatcgcgg acgccacgcg cccgtccggc gccaattgtt
     4681 tcggctccaa cattcccaac tctccaatct ctcaggcggt tgccctctgc gcggaccgca
     4741 accgagccaa tagcactaca actataagtt gtaaacgtaa taaatagcac ctaatgcgtt
     4801 tctgacggct ttgcgggggt tgtttggtgc tgaaggcgcg agctcagccc ggcagcgtca
     4861 gttcaattcc agcaattccc ggaaataggc gattgtgatc gtcaaacctt cgtcgagcgc
     4921 gattttcgga gtccaatcca gcgtcttgcg cgccagcgca atgtcgggtt gccgctgccg
     4981 gggatcgtct gtcggcaacg gccgaatctc gatccgggaa cgtgtgcccg tcatgcgaat
     5041 gattttttcg gcaagttccc ggacgctgaa ttccgtggga ttgccgagat tgaccgggcc
     5101 ggtcagttcc gggggcgtgt tcatcagctt gatcagccca tccacgagat cgtccacata
     5161 gcagaaacag cgggtctggc tgccgtcacc gaacagcgtg atcgcttcgc cctttagcgc
     5221 ctgtacgatg aaattggaaa ccacccgtcc gtcattggga tgcatccgcg ggccataggt
     5281 gttgaagatg cgagcgatct tgatggggag ccgatgctgc cggtgatagt cgaagaacag
     5341 cgtctcggcg cagcgtttac cttcgtcgta gcaggagcgc ggcccgatgg gattcacatt
     5401 gccccagtaa gattcgggct gcgggtgaac cgacggatcg ccgtagactt cgctggtgga
     5461 ggcctggagg atgcgtgctt tcagcctttt ggcgagcccg agcatgttga tggccccgtg
     5521 cacgctggtc tttgtcgtct ggaccgggtc gcgctggtaa tggattggcg atgccggaca
     5581 agcaagattg tagatttcat cgacctcgac atagagcgga aatgtcacat catggcggat
     5641 gaattcgaaa cgcgggttgg gcagaagttt tgcgacattg cgccgcgagc cggtgtacat
     5701 attgtctacg cagatcacgt catggccctg ggccacaagc ttttcgcaca ggaacgagcc
     5761 tatgaagcct gcgccacccg tgacgagaat gcgtttggaa tcgtgcatgt cctgaatggt
     5821 ccatcctcgg atgttttttc ggtctgttgc cgcctgcggc aagtcctatg ataggagcgg
     5881 gttcggagga agaccatgcg cattcgcttt gacaagtttg tctattccgc ggccattggg
     5941 cttctggcgg caatgggtcc tgcgcgcgcc gccgcaaacg acgaggttcc cacagcgctg
     6001 atgtccgcgg cggaatgcat ggtgaggact ttgaaagcca tgcccggcgt cacagaggtg
     6061 caaatccgtg tgtcggcggg acagggcggc gcctatcccg ttttgcaata tcgtttcgcc
     6121 gatgctttcg gaggacgccg tttcaccgaa gtgagtctgt tcgaaatcgt gggcgctgcc
     6181 gacccctttg tgttcgacaa agcggacatc gatgaagatg tggtggccga gcggctgctg
     6241 cccgtatgga aggccagatg ccattcgggg ttcggttaca tcaccagcca gccgcaatga
     6301 ggaaggatct tacggatacg gacgagcctg tgcccatcca atttccaccg agacagaacg
     6361 gcgtatccgc gcgatagatt gtgggaggcg actgtgatcc gaaagctaaa atcgggcgaa
     6421 taccgcctct attctcgtaa gaaaaatccc aagaccggca agcgccggaa tttgggcacg
     6481 ttcaagacgc gttccgcggc ggaaaaacac gaacgcgcgg tgcaattttt caaacgaggc
     6541 ggcgcatgac gaaagccaca tcggaaatcg aacgtgacgg ctcgccgttc tggcgattct
     6601 ccttgcgttt ctatgcccgc gccaagatgt cggcggcgtg tctggtactg caggacgaag
     6661 cgggggtgga tgtgaatttg ctgctgtttc ttctgtttct tgcggagcat cagcgtcagc
     6721 tcggcgccgc ggatgtcgcc aggctggacg ccgcggttca cgattggcgc gatagcgtgg
     6781 tgaagccgct tcgcgcgctc agacgcgcgc tgaaaaccgg ggtcggagaa atttccgtga
     6841 cgattagcga aacctttcgt ggacagataa aacgtctgga actggattcc gagcacatcg
     6901 agcaatcggt gctggaacgc ttcaatgccg cggaattggg cgcaccagcc gtgtcccgca
     6961 tggcggcggc tgaaacgaat atcgcgtcct atgaaatcca tctcggacgt tttcccggcg
     7021 aggccaaagc gaacatcctg tcgggtttcg ccgaattcgg accgcatcat ccggcaaatt
     7081 gattgccttt aggcccgccg tttaaacgaa ttttagctcc cataatttat accggccttt
     7141 caaaggcctg ccctcatttt ccgcggcggt tgtgaacacc cgccttgaag gcctgcggcg
     7201 caacgcaatg gcacaggtga atcatgggga tagacatggg aaattccgat ctgatcgtgg
     7261 gagaggccgt gccgccgcgc acgatgcgcg agcaactgaa aaaccctgcc cggaaaaaaa
     7321 gcgccgcgcg cgttgcgccc gatgagcggg cgaaagaaac cgcttccgat acgaaagacg
     7381 cccccaccga tgtgatcgcg cgtctggagc ggcgccttgc cggcctcgag aagacgattg
     7441 ccaatatcgc caatgtcgtc gaacgccacg agatcacgct gcgcgaacgc gaagaagccc
     7501 tggcctccgt ggcgcaaagc gtggttgccc ttcgcagcca gatggagcag tccgagaaac
     7561 agcacgcggc agaaaccgcg gaattgcacg cggctctcgc gcaagccggc acgcggttga
     7621 atgcgttgga agccgccaaa gccgcaaccc cggcgccggc ggctgctatt cccggatcgg
     7681 ccatcgaagc agctccttgc gctccgccga tcgtgtgggc gggagaaaac ggcaacccgg
     7741 ccgccgctgc cgcgacgcgc gcgccgcaag tttccgcgac cggcaccaag gatacgcaag
     7801 ttccgtccta cctttcggcc gcgcgcagcg ccgccaatca tgggacaggc gtggtggaca
     7861 agaaggcggc cgtgggcgcg cgccgcatcg gccgtaccca attgatggtg ttcggctgtg
     7921 tcgcaccgct ggtgctgctc gcaacgggtg cattcgtcct gaaccatcac cccgtgacgg
     7981 ctgaaccggt gcccgcgaag accgctttcg tgccaccgcc ggaagcgcct ttgccgccgc
     8041 cgccgcaaat catcatcgcc ccgccatcgg acccgaccgc ggaggaactc gcgaattctg
     8101 cctctctcga tgcgctgcag cagaaggcca agaccggcga tgccgatgcg gagcgggaca
     8161 tcggactgaa atatctcgca ggcgacacta tccccgtgaa cgaagaagaa gccgcgcgct
     8221 ggctcctgcg cgcctcctat cgcggtgaac cgtcggccga atattggctg ggcacgctct
     8281 actcgcgcgg acgcggcgtt cccgccgatc tgccacaggc caatcattgg tacggcgcgt
     8341 cggccaagca gggcaatccg tatgccatgt accggttggg aatcgccaat ttcgagggcc
     8401 tcgggatgga cagtaatgcc gagcaggccg cgcactggtt cacccaggcc gccgagatgg
     8461 gcttgataga ttcccaattc gatcttgcgg tgctgtatga gcgcggcatg ggcgtgatgc
     8521 agagcctgcc cgacgcatac aaatggtaca cggtcgcggc gacccagggc gacaaggaag
     8581 ccggcgagcg cgcggcccag ttggccaagg cgttgaagcc cgcggagcta gcggacgcca
     8641 cgcgcgcctc cgcggagttc aaaccggaat tgcctgcggc agttgccgcc ccgaaataaa
     8701 gacgggtctt aacggtcatc gaaaaggcgg gtggcgtcag cgccatccgc ctttttcttt
     8761 ttgcgcgttg tccagccatc gcggagtgct aaactagaga gacgaatttc gcagcgagcg
     8821 aggatcatgc catgaggcca tttctcattg ccaccagtct cgttgcgctt ttgattgttg
     8881 cggcctccgg tcaagaacaa aagaaaacat caggagcgaa aatcccagcg ctcgcatcga
     8941 gcgatttcgg gtggcagacc aatttcgagg attggcaaga cccgccgcca ggctatgggc
     9001 atggtccaat gaagaacgat ccggcctatc ccttcgtcaa caattcggtc gtcgatgctc
     9061 ccgaaaacga cttggcaaag agccaggaca agaacttcat caacatcaat ggaaggcaac
     9121 cgactgtccg catcgccaac agcaaggacc ccatcctgaa gccgtgggcc gcggccgctg
     9181 tgaaggcgac caatgatgag atcctccaag gcaagcggca ggtctctttc gtggcgcaat
     9241 cgcgttgttg gccgggtggc gttcccggtc agctcattta tccgtttgaa ccgctctact
     9301 tcatccagat gccgaaggaa gtgtggatga tatggcagcg cgaccacatg atccgccgta
     9361 tctatctcac cggcaaacac tcggccgatc tcaagccgtc atggtttggc gaatccattg
     9421 gtcattatga gaacggcgat acgctggttg tggatactgt cggcctttcc gcgaacaagc
     9481 tcagctatat cgacaatttc cgcacgcccc attccgaaaa gctgcacgtc gtggagcgct
     9541 tcaggatcag cccggacaac aacaccctta ccgccatcgc gcgcgccgaa gattccgacg
     9601 cctataacgc gccgatcacc atgatgcggc aatggttcaa ggtaaacgag ccaatagcgg
     9661 aaacggtctg cgccgaaaat aatgacgacc acttccacca aaatctctac ccaatcccgg
     9721 aagccgaaaa tcccgatttc tgaatagcat tgagcgtgcc gtcttctatt ccgccgagca
     9781 tacatcgggc attggctgcc gcgtgatcgc cgcgcggtga cgccgacgcg aaatcgcgga
     9841 gagcggtcat aaagaaaggg gcagcgtgtg ctgccccttc tcccgccccc atcaagcgat
     9901 gcgttttatt cggtgacgat tgccaggtcg gcatggccca gcgcatgggc ctggtcgacc
     9961 gcggcattgg cttggcgaat gttgaggaac ggtccgatcc ggacgcgata gacagcatgg
    10021 ccatcgaccg tgccgctcat gacgcgcgca cccatgctgt cgaggttcga agccgacttg
    10081 accgcgcgct cttcggtcgg aaaggacccg gcctgaacat agaaggaact gctggatgcg
    10141 ggtgtgcgtg ccgctgccgt tatcagcctc ggcgcaggac cggccggtgt cgcgttgttg
    10201 ggcgcgggcg ccgcggcggg agctgccgcc actttgggcg ctggcgttga agcgtttacg
    10261 cttttgggag ccggagttgc tgtggctgcg gctttcggcg cgggcgcctg tacggctttt
    10321 ttctccggca ctgaggccaa ttcgggtttc gcgggcagtt catgcggcgc gggcgccgct
    10381 gcggtttcct tgggaagtgc ctgcatgcgc acgctgggat cggatttgag cgcctcgacc
    10441 gcgctcggct ccggcttcac cgccggtgtg ggcatctgcg tctccagagt cgtggtcacg
    10501 acgggggccg gaacgggcag cggagccact gcggttggga tcgccggcat agcagacgcg
    10561 ggcggggtga cggatgccat ctgaacgggc gcgggcgtcg cagtgggtgc aggcgcgggc
    10621 gtggcggcgg ccatggcttt ggggggttgt gccgctttca ggctctgcac caaagcgaga
    10681 ttctgcgcga tgcgcgcatc gtcgggcgaa ccgggcatcg cctgacgcag caattgctcc
    10741 gcgccatcca gatcgcccga ttgcatatgg gacagcgcgc tattgttgag caccgaagcc
    10801 tcgcccggct tcagcgtgag ggcgcgcgcg tagaatgttt gcgccgcctg ataattgcct
    10861 ttctgatcga gcgccacacc ttcggcggag tacaacgacc aatcgccggg gtccagctgc
    10921 gtggcccttt cgaggaatgc gagcgcgtcg tcgctgcgtc cctctgcggc aagcgccttg
    10981 ccatattcgc ccaggacatg cgcatcgtcg ggagtgacaa gcacgagctg gctcaggatt
    11041 ttggtcgcac cggccaggtc gccatttttg cgcgcggctt gcgcggagcg gatagcactt
    11101 tcgagatctg tgggcagtgc gctcggttgt ttggcgtcgg ttgcctgttt ggctttggcc
    11161 attggcgcgg gcgtcgtagc cgtcgacagc ggattgatcg cgcagcctcc aagcggcaaa
    11221 agcagaacgg caattccgag cagatccgtg cgtttcacga ttcgacactc ctttttgatc
    11281 cgcatgggcg gaaaaattcc attcgggcgc cgcgcgcaat tcttcgcacc cgtacccaga
    11341 tggtccacag catggaggaa cgtcgtcaag aaaggcttaa gccacggcag gaatggtcga
    11401 aaaatgcgtt cggccgcggc catggcgctg ttgccgcctg ggcggcttgg gctatggtcc
    11461 cgtcgccaag tagaagagtg tcgccatgac gatttcggtg ccggaggccg ggcggaccgc
    11521 ccaacagaag tcaaaaatcg ccaaggattt tcaggaacat ctggcagccc tggaggctcg
    11581 cgggttggtt gtgcgggtgg accgcccgat caacaaggac accgagctgc atccgctggc
    11641 acgctggcag ttccagggcg ccattcccga ggacaagcgg cgcgctttcc ttttcaccaa
    11701 tgtggtcggc agcgacggaa aaaaattcga catgccggtg gtcgcgggcg ccttcgccgc
    11761 aagtccggaa atctatgcac tcggcatggg ggtcgcgctt gaggacatcg gtccggcttg
    11821 gctgcgcgcg attcagaatc cgatttcccc cgtcacggtg aaatcggggg catgccagga
    11881 ggtggtgatc gacggggacg cgttgaagac gcccggcggc ggattggctg ctctgccggt
    11941 gccgatttcg acgcccggct tcgatgcggc accctatctg accgcaacca tctgcgtgac
    12001 caaggacccg gaaacgggcg tgcggaacat gggcacctat cgcggccagt tgaaggcgac
    12061 cgaccggctc ggcgtgcgca tggcctcgcg gttggggggc gcgggcggtt atctgcattg
    12121 gttgaaatat caaaagctgg gccagcccat gcccgtggcc atcgtcatag gctgcgcgcc
    12181 ggtaattttc ttcaccggcc cccaaaagct tggcatcgat ttcgatgaga tgggcgtggc
    12241 cggcgcgttg ctgggtgaac ccgtcaagac tgtgcgctgt gtcaccaacg atctcgaagt
    12301 gcccgcggat tccgaaatcg tgatcgaggg cctgatcgac tcgcaattgc tggagcccga
    12361 agggccattc ggcgagtcgc acggccacgt cgcactcgaa gactacaata tgtcgatgca
    12421 ggtcaccgcg atcacgatga agaagaagcc cgtcttcgtc tcgatcatca gccaggtgac
    12481 gccatccgaa tcttccgtga ccaaggtggt cgcctatgag cctttgttcc tcgcgcatct
    12541 gcggggccat ttgggggtca aggggatcaa gcgcgtcgtc atgcatgaag cgttgaccaa
    12601 tgtgcgcccg gtgatttttt tgaccttcgc gcacggcaca ccgaagaccg agatctggcg
    12661 cggcctccag ggggccgcga cattgcaggc ccaatgcggc aagatcgtta tcgcggtcgg
    12721 cgaggacatc gatcccacca acaccgatgc cgtcttctgg tcgctcgcct accgcaccaa
    12781 tctcggcgag gatttgcatg tggcgcccta taaggacgcc ggacacggcc cgaaatcagg
    12841 cggggcacgg agcagcgatt ccacgttgct ggtcgatgcc acccccaaac acgcgatgcc
    12901 gcctttcgca ctgccggcgc gtgaattcat ggaacgggca aaagtgatct gggacgaact
    12961 cggcctgccg gaactcaatc cgcggccgcc ctggtatggc tattcgctgg gcgattggac
    13021 ggccaattgg gacaattggg ccaagcgcgc ggtggaaggc agatgggaag aaacgggtgc
    13081 tgagacctat gcgggccgca aaggcgggat gaaacccgaa accccggtcc gcgacgtcaa
    13141 ggccaaggaa aagtagagct acttcgcggc gaatttcggg cgtgggcgtt ggtcttgcga
    13201 aatctcctgc acgagtttgc gcatgatcgc ctcgccgaac gcattgtaat ccttgaccgg
    13261 aatgatgaac gcgccgggtc caccaatcac gctttcgcgg aaatactgat cgaggtccgg
    13321 ttcgacggcg agaatgggaa gcccgttgat acgcacgccc atcttgaccg cttcgtcgcg
    13381 cgcctgttcg gcgggccgtc ccagattgtt cgatccatcg ccggaaatgt cgatgacctt
    13441 gcgttcggcg cgataggggc tcgcggtcag catcgtgacg gaaaaatcga tagcgcctga
    13501 gagcgatgtc ccaccgccga agatttgacg cggcgaggcg gcgatggccg cggccgccaa
    13561 ttgcgccgag cgctcatcct tgatcaccat ccacggtaac atgatcacgt gcagcgttgg
    13621 accggtccat tgcaccatgg aaatggcaat cgcggcctca ttgccgttgc ggatggcatt
    13681 cagcacgcga ggattgagaa aggctttcgc atagccctgc ttctgcaact cgaagcggtc
    13741 gtcatccacg ctgcctgagg cgtccaccgc aagcacgaga ttgagatcca cgcggggttg
    13801 tccggccggc tgcgtgatca ggccgcgcga gggcgtgggt gccggcggct tgggcgcggc
    13861 aggttgagct tgcgccaacg tgacgcttag ccctacggca aacagcaagg ccgcggtcca
    13921 gcccaatccc tgagaacggt tcattcctca tcccttccgt tcggatgcgc ctaaattact
    13981 gggacatgcg cgccgggcaa gaaaattcgc tgcaatatgg cgcgattttg cgggcgcggt
    14041 gatgcaccgc cataggcgtg ggcgtctttc tcgtgatact ggcatcaaaa agccgctggc
    14101 ggtgggccgg gcagttaggc cgggcagttt gggagacagg accgtgcaaa ccgacgcatt
    14161 tcgaatcgaa gaagacagag cgcggcacgt cgcgacggta aggctgacgc ggggtgctgc
    14221 cggcaacaaa ctcagtgcgg cggaaatccc gctattgggc cgcgccattc gtgaaatggg
    14281 c
//
LOCUS       DASYEG010000067         5801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4514444, whole genome shotgun sequence.
ACCESSION   DASYEG010000067 DASYEG010000000
VERSION     DASYEG010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4514444"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1660)
                     /locus_tag="VGM72_02570"
     CDS             complement(<1..1660)
                     /locus_tag="VGM72_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY4264174.1"
                     /translation="MAQGSQPYVEDLQRQARTRAPSKKLTPLAALKPFLMPYRGMIAA
                     AGLALILAAMATLVLPAAVRGMIDHGFSQADAANIGRYFLALIGVVAVMGIASASRFY
                     LVTWIGERVVADVRARVFSHVLSLSPRFFETTRTGEVLSRLTADTTLVQTVIGSSASI
                     ALRNLLMALGSLVMMTITSPKLTGLCLIGVPLVVAPIILFGRRVRKLSRDSQDRIADT
                     AAYASEALNAVQTVQAFTHEDVDRMRFGAAVEDSFIAAVRRTKMRAAMTALVIFLVGA
                     GIVIVLRVGANDVLAQRMTGGELSQFILYAIFLATGMGAVSETWGDVQRASGATERLM
                     EIVSTEPEVTAPANPRALPAPAHGAVVFDDVTFRYPARPEQKALHSVSFAISPGEAVA
                     LVGPSGAGKSTMFQLLLRFFDPQEGRILFDGAPIAEADPRALRRSIALVAQDPAIFAG
                     SIADNIRYGRPEASDAEIREAAMAAAADEFIRRVPEGYEAMLGERGVTLSGGQRQRLA
                     IARALLLNPRILILDEATSALDYESERVIQENMKAISAGRTVFII"
     gene            1809..3335
                     /locus_tag="VGM72_02575"
     CDS             1809..3335
                     /locus_tag="VGM72_02575"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4264175.1"
                     /translation="MSATHPTATRRPLVLVCVMLALFISAIEATIVATAMPQIVGKLG
                     GFALYSWVFAAFLLTQTATTVLFGKLSDIYGRKPVMIGGILIFLVGSLLCGFARSMPA
                     MIVFRLIQGLGAGSMQPVAITIAGDIYAPRERLRIQGWLSAVWAIAAIAGPIAGGLIV
                     QRFNWAWVFWVNIPIGILTIIGFLLFMQEDIQTKTHTIDYPGALLFFVSVTALLVALT
                     QGATLSWAEIGGLVALFATASLLFLLQEKRAPEPMIALDLWGMRLVATANASLLLGTM
                     TLIGVTSYLPVYIQAVQGRPAILAGIPLTAMLFAWPLASAFSGRILKRISMRKTLRFG
                     ALLIPTGALFLLPIRPDSHPAFAAVGPFIMGFGMGLLNITSMVMIQGSVVWSKRGSAT
                     ASLIFSRTLGNTLGVTALGAILNFGVVLFASEHARMSTDQIHALLGSIGNILGGGANP
                     DLHAALDAALRLTFWGMAAFAILAAVFAMMIPVRELETLSAHGGSTAPAASPTPPAGE
                     "
     gene            complement(3409..4272)
                     /locus_tag="VGM72_02580"
     CDS             complement(3409..4272)
                     /locus_tag="VGM72_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264176.1"
                     /translation="MSGALLCAAAALLPARAADAIPDFMADGMGWASAGGLTAVPGSP
                     SPVVQDPAVKYVPNNVGGQPTWRVGDVNNPNLTQFAKDGLRKANDMVAEGFALYNRTS
                     RCWEPGIVSLNLSPGRTYFLQTPKEVTIIWQRDQIARHVYLNVPHSANPKPSWNGESV
                     GHYEGDTLVVDTIGQTTKSFVDLYRTPHSEKLHVVERYRMLDGGKKLQVEMTVDDPVA
                     FVQPWKGTKIWNKDTTRPKVDGNVTGTFAEEIRCMDGEMVNPLNQEYAAKLEPLPVDR
                     SGTEQKLLSGG"
     gene            complement(4511..5563)
                     /locus_tag="VGM72_02585"
     CDS             complement(4511..5563)
                     /locus_tag="VGM72_02585"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY4264177.1"
                     /translation="MHAQSDSPNSEHGPSRRNVLSGIGLAAAVAALPPIAARAATANV
                     TFGGAQGFFNKEYTAKKGDVKLALYRRRAKEPVAGAAPLPVLIMVHGSSIGALSSWDL
                     NVPGAGEYSMMNVFARFGYDVWAIDFEGYGKSDVTAGNSDIKSGVADLIAAVPLIAQE
                     TGQQKFHFMGESSGALRVAAFAAAHPERMDRALLGAYTYTGKDSPTLTERAKQVDFYR
                     THNRRPRPKAMIESIFTRDHPGTSDERVSAAMVASELRFGDSVPTGTYLDMTAKLPII
                     EAKLVKCPVLLARGQYDGIATEADILDFYSQLPNPDRQLSILPGLAHSLTLGLNRQIY
                     WHVANAFLSMPKGQFV"
BASE COUNT         1045 a         1856 c         1804 g         1096 t
ORIGIN      
        1 cgatgatgaa gacggtccgg ccggccgaaa tcgccttcat gttctcctgg atcacccgct
       61 cgctctcata gtccagcgcg ctggtggctt catccaggat caggatccgc gggttcaata
      121 atagcgcccg agcaatcgcg agacgctggc gctgtccccc ggacagggtc acaccgcgct
      181 cgccaagcat cgcctcatag ccctccggca cgcggcggat gaattcatcg gctgccgcgg
      241 ccatcgccgc ctcgcggatt tcggcatcgg acgcttcagg ccgcccatag cggatgttgt
      301 cggcaatgga gcccgcgaaa atcgccgggt cctgtgccac cagcgcaatg ctgcgccgaa
      361 gcgcgcgcgg gtccgcctcg gcgatgggag cgccatcgaa caatatccgg ccctcctgcg
      421 gatcgaagaa gcgcagcagc aattggaaca ttgtcgattt gccggcaccg gacgggccga
      481 ccagcgccac cgcttcgccg ggagaaatcg cgaaactgac actgtgcagc gccttctgct
      541 cgggccgtgc gggatagcgg aaggtcacat catcgaacac gaccgctccg tgcgcgggcg
      601 ccggcaatgc gcgtgggttc gcgggtgccg tgacctcggg ttcggtggag acgatctcca
      661 tcagacgctc ggtcgcgccc gatgcgcgct ggacatcgcc ccaggtctcg ctcaccgcgc
      721 ccatgccggt cgcgaggaaa atggcgtaga ggatgaattg gctcagttcg ccgccggtca
      781 tgcgctgggc cagcacatca ttggccccga cgcgcagcac gatgacgatc cccgctccta
      841 ccagaaagat cacgagcgcc gtcatcgccg cacgcatctt ggtccggcga acggcggcga
      901 tgaaggaatc ctccaccgcg gcgccgaacc tcatgcggtc gacatcctca tgggtgaagg
      961 cctgcacggt ctgcaccgcg ttcagcgcct cgctcgcata ggcggcggtg tcggcgatcc
     1021 ggtcctggga atcgcggctg agcttacgca cgcgccggcc gaacaggatg atcggcgcca
     1081 ccaccaacgg cacgccgatg aggcaaaggc cggtcagctt tgggctggtg atcgtcatca
     1141 tcacgaggct gcccagcgcc atcagcagat tcctgagcgc gatcgaggcc gacgatccga
     1201 tcacggtctg cacgagcgtg gtgtcggcgg tcaggcgcga cagcacttcg ccggtgcgcg
     1261 tggtctcgaa gaaacggggc gagagcgaca gcacatggct gaagacgcga gcccgcacat
     1321 cggccaccac acgttccccg atccaggtga ccaggtagaa ccggctcgcc gacgcgatgc
     1381 ccatcacggc aacgaccccg atcagggcca ggaaatagcg cccgatattg gcggcgtcgg
     1441 cctgcgaaaa accatggtcg atcatgccgc gcaccgccgc gggcagcacc aaggtcgcca
     1501 tggccgcgag gatgagggca agccccgccg cggcgatcat cccgcgatag ggcatcagaa
     1561 atggcttcag cgcggcaagc ggcgtgagtt tcttcgacgg cgcgcgggtc cgtgcctgac
     1621 gctgcaaatc ctcgacatat ggctgggagc cttgggccat gggctcctat agccgaaggt
     1681 ttccgcgaga gccacatgaa cccggaacac gtctttgggc gtgacgccat gcgcgggcgt
     1741 gctagaagac acgcgcaatc gaacgccttt gcgcgcgtct tcaggtcctc gccggccaaa
     1801 gagaagaaat gtccgccaca catcccaccg ccacgcgccg ccctctggtc cttgtctgcg
     1861 tgatgctggc gctcttcatc tcggccatcg aggcgaccat cgtcgccacg gccatgccgc
     1921 agatcgtcgg caagctcggc ggcttcgcac tctattcctg ggtgttcgcg gcattccttc
     1981 tgacgcagac cgcgaccacg gtattgttcg gcaagctgtc cgacatatac ggccgcaagc
     2041 cggtgatgat cggaggcatc ctgatcttcc tggtgggatc gctgctctgc ggctttgccc
     2101 ggtcgatgcc cgccatgatc gtcttccggc tgatccaggg tctcggcgcc ggctccatgc
     2161 agcccgttgc catcaccatc gcgggcgaca tctacgcacc gcgcgagaga ttgcgcatcc
     2221 agggctggct gtccgccgtc tgggcgatcg ccgccattgc cggccccata gccggcggcc
     2281 tcatcgtgca gcgtttcaac tgggcctggg tgttctgggt gaacatcccc atcgggatcc
     2341 tgaccatcat cggattcctg ctgttcatgc aggaggacat ccagaccaaa acccacacca
     2401 tcgattaccc gggcgcgctg ctgtttttcg tttcggtcac cgcgctcctg gttgcgttga
     2461 cgcaaggcgc gacactgagc tgggccgaga tcggcggctt ggtcgcccta tttgccaccg
     2521 caagtctgct gttcctgctg caggagaagc gcgcccccga gccgatgatc gcgctcgatc
     2581 tttggggcat gcggctggtc gcgaccgcca atgcgtccct gcttttggga acgatgacgc
     2641 tgatcggcgt gacgtcctat ctgccggtct atattcaggc cgtacaaggg cgccccgcca
     2701 tcctggccgg catcccgctc accgcgatgc tgttcgcctg gccgctggcc tcggcatttt
     2761 cgggacgcat cctgaagcgc atctcgatgc gcaagacgct cagattcggg gctttactca
     2821 tcccgaccgg cgcgttgttc cttttgccga tccgtccgga cagtcacccc gcctttgccg
     2881 ccgtcggccc cttcatcatg ggattcggca tgggtctcct caacatcacg tccatggtga
     2941 tgatccaggg cagtgtcgtc tggagcaaac gcggcagcgc gaccgcttcg ctgatcttct
     3001 cgcgcacact cggcaacacg ctcggcgtca ccgcgttggg ggcgatcctg aatttcggcg
     3061 tggtgctgtt cgcgagtgaa catgcccgca tgagcacaga ccaaatccac gcgcttctgg
     3121 gaagcatcgg aaacattctg ggcgggggtg ccaatccgga cctgcatgcc gccctcgatg
     3181 ccgcccttcg gctcaccttc tggggcatgg cggcgttcgc gatcctcgcc gcggtgttcg
     3241 cgatgatgat tcccgtgcgc gaactcgaaa cgctgtcggc gcacggagga agcacggcgc
     3301 ccgccgcttc acccactcct ccagcagggg aataagagac tttcgaggga tgaccggccg
     3361 gcacagtcgg caggtcatcc agggcgtcat ggcgagggaa tcccgacctc agccgccact
     3421 caaaagtttc tgttcagtgc cgctgcgatc gaccggcaac ggctctagtt tggcggcgta
     3481 ttcctggttc agaggattaa ccatttcccc gtccatgcag cgtatttcct cggcgaatgt
     3541 gccggtgacg tttccgtcga ctttcggtct ggtggtgtcc ttattccata tcttcgtgcc
     3601 cttccagggt tggacgaagg caaccggatc gtcgaccgtc atttcgacct gcagcttctt
     3661 gcctccgtcg agcatccgat agcgctcaac cacatgcagt ttttcgctgt gcggcgtgcg
     3721 gtaaagatcg acgaagctct tggtggtctg cccgatggta tcgacgacca gcgtgtcgcc
     3781 ctcgtaatga ccgaccgatt ctccattcca ggacggtttc gggttggcgc tgtgcggcac
     3841 gttcagatag acgtggcgcg cgatctgatc gcgctgccag atgatggtga cttccttcgg
     3901 cgtctgcagg aaataggttc gtcccggact gagattgagg ctcactatgc cgggctccca
     3961 gcaacgcgag gtgcgattgt agagagcaaa accctctgcc accatgtcat tcgccttcct
     4021 caaaccgtcc ttggcaaact gcgtgagatt gggattgttg acgtcgccga cgcgccatgt
     4081 cggctgaccg ccgacattgt tcggcacata tttcacggcg ggatcctgca ctacggggct
     4141 cgggctgcct ggcacggcgg taaggccgcc agccgacgcc catcccatgc cgtccgccat
     4201 gaagtcaggg atggcatcgg ccgcgcgcgc cggcagaagt gcggcggcgg cgcaaagaag
     4261 tgcgccgctc aaaccgcagg ctgcaaagcg cagatttttg tttctgtgaa agccaacccc
     4321 agtcatgcgc atctctcccc gattgcgtga acaggacact ctcgggggat attacgcccg
     4381 ccctgtcccg ctgacaacga agcttgccgg tgcttactgg ggagaaaaag aaacggcccc
     4441 acggacatgc caatggggcc gcttactcag tcgttcaccc tgcgacacgt tcaggatgag
     4501 gagcttgacc tcagacgaac tggcccttgg gcatggacag aaatgcgttg gccacatgcc
     4561 aatagatctg gcggttgagg cccaaggtca gcgaatgcgc cagcccggga aggatcgaaa
     4621 gctgacggtc cggattcggg agctggctgt aaaaatccag gatgtccgct tcggtcgcga
     4681 tgccgtcata ttggccgcgc gccagcagca ctgggcattt caccagtttg gcttcgatga
     4741 tcggcagttt cgccgtcatg tcgagatagg tgccggtggg gacggaatcg ccgaagcgca
     4801 attccgaggc caccatggcc gccgacacac gctcatcgga cgtaccgggg tgatcgcgcg
     4861 tgaagatgga ctcgatcatc gccttggggc gcgggcggcg attgtgggtg cggtagaagt
     4921 cgacctgctt ggcacgctcg gtgagtgtcg gcgaatcctt gccggtatag gtgtaggcgc
     4981 cgagaagcgc ccgatccatg cgctcgggat gggccgcggc gaacgcggcc acgcgcagcg
     5041 caccggagga ttcgcccatg aaatggaatt tttgctggcc ggtttcctgg gcaatcagag
     5101 gcacggccgc gataagatcg gcgacgccgc tcttgatatc ggaattgccc gcggtgacgt
     5161 ccgatttgcc atagccttcg aaatcgatcg cccacacatc gtaaccgaaa cgcgcgaaga
     5221 cgttcatcat gctgtattcg ccggcacccg ggacattcaa atcccaactc gacagcgcac
     5281 cgatcgaaga accgtgcacc atgatgagca ccggaagggg agcggcgccg gccaccggtt
     5341 ccttggcgcg gcggcgataa agcgccagct ttacgtcgcc tttcttggcc gtgtactcct
     5401 tgttaaagaa accttgcgcg ccgccgaatg tcacattcgc ggttgcagcg cgcgcggcaa
     5461 tcggcggcag cgccgcgacc gcggctgcga gacctatccc ggaaagcaca ttgcggcggc
     5521 tcggaccatg ttcggagttc gggctgtcgg attgggcgtg catgcgggtt gcctccttgg
     5581 gttgaccgcc gcttaagggc gttagcccca gtttacgtgt ttcgtgacgc gaaggccaat
     5641 ccccaaagga gcccgagccg ggattttcgc gagggccgcg atgcggcgtt ccgtagacgc
     5701 aatgagaaaa atttcatctg cgttccggca ctggccgtaa cttcgagccg ggagtatcgt
     5761 tgtcccaagc ggatgcccgg cagaggtaac gaatggccgc t
//
LOCUS       DASYEG010000068        11068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3210130, whole genome shotgun sequence.
ACCESSION   DASYEG010000068 DASYEG010000000
VERSION     DASYEG010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 11068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11068
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3210130"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VGM72_02590"
     CDS             complement(<1..193)
                     /locus_tag="VGM72_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY4264178.1"
                     /translation="MDIEIIFAGDIARVKLRGRLDTPGVDKIESKFTTSVVPDSRNTI
                     VDLRDVSFIASMGLRMFIGI"
     gene            complement(260..1393)
                     /locus_tag="VGM72_02595"
     CDS             complement(260..1393)
                     /locus_tag="VGM72_02595"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEY4264179.1"
                     /translation="MSQSEENLSDRTTIYRRENTAPVSVHEEFGHYLVAIGGSGLGRR
                     LRLDATPLIIGRDLQCGLVLASLDVSRRHCQVELKGVEVIVTDLNSTNGTYVDGNRIA
                     APTALPDGSILEIGQQVLKHERRSTREVEEAQELDRDLEKARSYVLSLLPAPIAASTP
                     QTEWFIVPSARVGGDAFGYGELDEGKFVSFLFDVSGHGIGAAMLSVSILNVLRQHALP
                     GCDFKSPAQVLARLNAMFQMENHNGMFFTIWYGVYDRRQHQMTYASAGHHPAYLVSPD
                     RTKITPLKTEGALIGVLEEFTVKEATIDVPQNAALHLFSDGVFEVDDATGRQLGLPNL
                     LSLLHQPAVEGRSEPQRIYQSVREFARPGPLDDDFSYLVVNFP"
     gene            complement(1511..3067)
                     /locus_tag="VGM72_02600"
     CDS             complement(1511..3067)
                     /locus_tag="VGM72_02600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY4264180.1"
                     /translation="MKTRAAVLLPLALGLWGCELGPDYMRPSAIEPGDYKENRGWMPA
                     SPQQAAGWESWWAIYNDPILDGLEKQVDISNQNLRSAEAAYRAARAEVGIERGALLPA
                     LSANASVRQSGGGSRNTVITSTGTSDTTGTGTPVIASSGGSRTTYQTSLSGSWDIDVW
                     GRIRRTVEGGVATAQASAADIAAARLSAQSELAVDYFQLRAADEQAQLLVSIIQDFET
                     ALQIARNRVQVGITTQADVFAAQTQIDNAQAQLVNLQLTRAQLEHAIATLIGKPASDV
                     AIAKAPLPTIIPVVPTGVPSTLLERRPDIAASEYTMASANAQIGVAIAAWYPDLTLTG
                     SYGFASSSLGSLFSAPNSVWSLGPSLAELVFDGGAREAQTQQARARYEQSVAIYRQTV
                     LTAFQQVEDQLAALRVLEQEAAVENQTVSDARQSEQLALNQYRAGTADFTTVITAQTA
                     RLNAESSALSVLDQWLAASINLIVALGGGWDNSRLPQPSFFYRLPEMTTNDEPPPQDA
                     VPAPAPANAR"
     gene            complement(3064..6405)
                     /locus_tag="VGM72_02605"
     CDS             complement(3064..6405)
                     /locus_tag="VGM72_02605"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY4264181.1"
                     /translation="MNVSARFIARPVATTLLTIWIAIAGGAAFFLLPVSPLPNVDYPT
                     ISVTAQLPGASPETVATSVATPLERHLGTIADVTEMTSSSSVGQVRITLQFSLDRDID
                     GAARDVQAAINAARVDLPAALRSNPTYRKVNPADAPILILALTSDTLTPGQIYDSAST
                     VLQQKLSQVDGVGEVQIGGSSLPAVRVELNPRALFKYGIGLEDVRAALSAANANAPKG
                     AIEQRERRYQIYVNDTATKAAQYQTLIVAYRDGAPVRLSDIAEVSDGVEDIRNQGNAN
                     GKPAVLVILNRQPGANIIDVVDRIKAMIPSLQASIPNSITITPVNDRTTTIRASLRDV
                     ETTMGIAVLLVIAVVFVFLRNGRAALVPSVAVPISLLGTFGVMYLLGFSLDNLSLMAL
                     TVATGFVVDDAVVVLENISRHMEEGMPRMQAALLGAREVGFTVLSMTVSLIAVFLPIL
                     LMGGLIGRLFHEFAMTLSIAILISLVLSLTTTPMMCAYLGMGRAKREQGWLMRGSERV
                     FVRVHRAYDRSLSWSLSNPGTIMVILALTIGLNVYLFTIIPKGFFPQQDTGSIGGGIR
                     ADQSISFQAMKGKFDQFVNAIHQDPAVQSVVGFSGRGGQTNTGNVFVNLKPLSERKET
                     TDHVIARLRTRLGNVSGARLFLAPVQDLRVGGRQANAQYQYTIQSDTLAELETWVPRI
                     TEALQDVPELLDVNSDRQEAGLDVELHIDRSTAARLGVTATQIDNTLYDAFGQRQVST
                     IYKDLNQYHVVMEVAPAFWQNPETLRDVYVSTAGNITGTQASGGIVTTAAAPAESNTA
                     GSVGAATSTTAAAAATDLSVADATAFQNQAQNQIANTARGGASAGASVSTRVERMVPL
                     AAFSSYDAGTTPLSVNHQGPFVATTFSFNLPEGESLSDAVSAIQRTMATLHVPISVHG
                     TFAGTARSFQQALAYEPLLILGALVTVYIVLGILYESLVHPITILSTLPSAGVGAVLA
                     LLIFGAEFSLIALIGVILLIGIVKKNAIMMIDFALVAQRTEGLDSREAIYRAALLRFR
                     PIVMTTLAAIFGALPLALITGDGAELRQPLGISIVGGLLVSQLLTLYTTPVVYLYLDR
                     FRLWSAGLWKRSYGGLFGRPREATP"
     gene            complement(6402..9563)
                     /locus_tag="VGM72_02610"
     CDS             complement(6402..9563)
                     /locus_tag="VGM72_02610"
                     /inference="COORDINATES: protein motif:HMM:NF007798.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtB/MuxB family multidrug efflux RND
                     transporter permease subunit"
                     /protein_id="HEY4264182.1"
                     /translation="MSADSNPNETGPDETGINPSRIFIQRPIATSLFMVAILLVGLVG
                     YFFLPLSALPEVDYPTIQVQTFLPGASPEVMTTSVTAPLERQFGQMPGLREMSSASSA
                     GASVLTLQFDLSLNLDIAEQEVQAAINAAGNLLPQNLPAPPVYAKVNPADAPILTLAV
                     SSASLQMTQLEDIADTRIAQKISQLPGVGLVSIGGGQRPAVRVQANIQALAAYGLNID
                     DLRTIIGNANSNAPKGNFDGATRAYTINANDQLQTPEDYRSIVVAYRNGAPVHLSDVA
                     DVIESAENVKLSSWMNMTPAILLNVQRQPGANVIAVADSVKNLLPDIKTSLPASIDVT
                     VLTDRTNTIRASVADVEFELGLAVALVVLVIFLFLRNVPATIIPSLSVPLSIVGTFAA
                     MYLLGYSLNNLTLMALTIASGFVVDDAIVMIENISRHLEMGEKPMEAALKGSGEIGFT
                     IMSLTVSLIAVLIPLLFMQEVVGRLFREFAVTLAITILISAVVSLTLVPMLCAKLLHP
                     EAEQKQGRIARKAGEWFDRGIAQYGRLLDWVLDRQFLTLMVAIVTLVVTVVLYIGIPK
                     GFFPVQDTGFIQAVTRAAPTVSFATMAERQQALADIIRKDPDVVSLSSFIGVDGSNTT
                     LNSGRFLINLKSKSERSTSVTDVMRRISRATHDVSGIALYMQPVQDLTVESTVGRAQY
                     QFALQAAQDATLAQWVPRLMDALARTPEIENISTDYLDRGLSAYIAVDRDTAARFGIT
                     AATIDNALYDAFGQRIISTIFTQSNQYRVILEADPDVQNSLASLETIYLPSSAGGQVP
                     LSAIAKIEERTGPLQIGHIGQFPAAMISFDVAAGGSLGAAVEAVRAVEAQVGLPPEIT
                     TSFQGAALAFQSALGNELLLIVAAIVTVYIVLGVLYESFIHPVTILSTLPSAGVGALL
                     ALIVSGHDLDIISIIGIILLIGIVKKNAIMMIDFALVAEREEKKPPREAIHQAALLRF
                     RPILMTTVSALFGALPLMLGGGTGAELRHPLGIAIVGGLLVSQLLTLFTTPVIYLYFD
                     RLATRSRSRRNAVPPEGARGVAP"
     gene            complement(9563..10987)
                     /locus_tag="VGM72_02615"
     CDS             complement(9563..10987)
                     /locus_tag="VGM72_02615"
                     /inference="COORDINATES: protein motif:HMM:NF008589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HEY4264183.1"
                     /translation="MDQRTDRKPFDAASDDITRRRGIDRIPGGRKIVIITIAVALVAL
                     LVWTIRPSRNAIPNRNAFAGANQATPVGIAKVTSGDITVTLNALGTVTPLATVTVKPQ
                     VGGQLIRIDFTEGETVRAGQVLAEIDPKPYQAALDQADGQLARDQAQLANAQVDLERY
                     RTLAGQNSIAQQQVDTQAALVRQLQGVIKSDQANVEAAQINLNYATIRSPITGRVGLR
                     QVDLGNLLTAGQATGIAVVTQLEPISVVFAVPEDAISDVLAQMRQGAKLTSDAYDRAQ
                     SKKIATGVLATIDNQIDTTTGTVKLRAMYDNKSGELFPNQFVNIRLLVNTLHNQMVVP
                     ASAIQHGASGTFVFVVNSDHTVTMRNVQVGQTQDDRVAVTSGVMAGDTLVVDGADRLR
                     DGAKVLLPGEQPPAAAATQGRAPANGAGGRRQGRAGRRGGQGADAPGAAPGTPDAGAA
                     GAGAAGAGAAGAGAAGGRGGPGGG"
BASE COUNT         2151 a         3355 c         3517 g         2045 t
ORIGIN      
        1 cgatgccgat gaacatcctc agacccatcg aggcgatgaa actgacatcc ctcagatcga
       61 cgatggtgtt tctgctgtcc ggcaccaccg aggtggtgaa tttcgattcg atcttgtcga
      121 cgccgggggt gtccagccgc ccgcgcaatt tcacgcgcgc aatatccccg gcgaatatga
      181 tttcgatgtc catgtcttga tctctgccca gcaaccccac gcaattctat accacgcccg
      241 tcagcgggct tgcattgcgc tatgggaagt tcacgacgag ataggagaaa tcgtcgtcca
      301 ggggccctgg gcgcgcgaat tcccgcacgc tctgatagat ccgttgcggc tcgctgcgtc
      361 cttccaccgc cggctggtgg agcaatgaca aaaggttcgg cagtcccaat tgccggcccg
      421 ttgcgtcgtc cacctcgaac acgccgtcgc tgaaaagatg cagcgccgcg ttttggggca
      481 cgtcgatggt ggcctccttg acggtgaact cctcaagcac gccgatcaag gcaccttcgg
      541 ttttcagcgg cgtgatcttc gtgcggtccg gtgaaaccag ataggcggga tgatgaccgg
      601 ccgaagcata ggtcatttgg tgttgccgcc ggtcgtagac gccgtaccag atggtgaaga
      661 acatcccatt gtggttttcc atctggaaca tggcgttcag ccgggcaagc acctgggccg
      721 gactcttgaa atcgcaaccg ggcagcgcat gctgcctgag cacgttgagg atcgaaaccg
      781 acagcatcgc cgccccaatt ccatgccccg acacgtcgaa gagaaagctg acgaatttgc
      841 cttcgtccag ctcgccatag ccgaaggcat cgccgcctac gcgcgcggat gggacgatga
      901 accactcggt ttgcggcgtg gacgcggcga tgggcgctgg cagaagcgaa agcacatagc
      961 tgcgtgcctt ctcgagatcg cggtccagct cctgggcctc ctcgacttca cgcgtgctgc
     1021 ggcgctcatg cttcagcacc tgctggccga tttccagaat gctgccatcg ggcagcgccg
     1081 tcggcgcggc gatgcgattg ccatcgacat aagtcccgtt ggtcgagttg agatcggtga
     1141 caatgacttc gacgcctttg agttcgacct ggcagtggcg gcgtgaaacg tccaggctcg
     1201 ccagcacgag cccgcattga aggtcccggc cgatgatcag gggcgttgcg tccaggcgca
     1261 aacggcgccc caaaccggac ccgccaatgg ccacgagata atgtccgaac tcctcgtgga
     1321 cgctgaccgg agcggtgttt tcccggcgat agatggtcgt ccggtcgctc agattctctt
     1381 cagactgcga catggattcg cctgcgaaga tgttggggaa gacaccccca tcagcttaac
     1441 ggcttggttt cgcgcgaaca agtaatgggc tgtgacggcg atgacgcagg gagtctccgc
     1501 gacagaattc ctatcgcgcg ttcgcaggcg ccggtgcggg tacggcatcc tggggcggcg
     1561 gttcgtcgtt tgtcgtcatt tcgggcaatc gatagaagaa gctaggctgc ggcagacgcg
     1621 aattgtccca gccgcctcca agcgccacga tcagattgat gctcgcggcg agccactggt
     1681 cgagcacgct cagggccgag ctttcggcgt tcagccgtgc cgtctgcgcg gtgatgacgg
     1741 tggtgaaatc cgccgtgcct gcgcgatatt gattgagcgc aagctgctcg gactggcgcg
     1801 catcggaaac agtctggttt tccaccgcgg cctcctgctc aagcacgcgc agcgcggcga
     1861 gctgatcttc cacctgctgg aatgcggtga gcacggtttg gcgatagatg gcaaccgact
     1921 gttcatagcg ggcccgtgcc tgctgggtct gcgcttcacg cgcccccccg tcgaacacca
     1981 gttcggcaag agaagggcca agcgaccaca ccgaattggg tgcgctgaac agcgagccca
     2041 gcgaggaaga ggcgaagcca taggaaccgg tcagcgtcag atccggatac caggcggcga
     2101 tcgcaactcc gatctgggcg ttggccgacg ccatggtgta ttcggaagcc gcgatatcgg
     2161 gccgccgctc gagcagtgtt gacggcacgc ccgttggcac gacagggatg atcgttggca
     2221 gcggcgcctt tgcaatcgcg acgtcgctcg ccggcttgcc gatcagggtc gcgatggcat
     2281 gctccagttg cgcccgcgtc agttgcagat tgaccaattg ggcctgcgcg ttgtcgatct
     2341 gggtctgggc ggcgaagaca tctgcctggg tggtgatacc gacctggacg cgattgcgtg
     2401 caatctgcag cgcggtctcg aagtcctgga taatggagac gagcaattgc gcctgctcgt
     2461 cggctgcgcg cagctggaaa taatccaccg cgagttcgga ttgcgccgaa agccgtgccg
     2521 ccgcgatatc ggcggcgctc gcttgtgccg tggcgacgcc gccttccacc gtgcggcgga
     2581 tgcggcccca aacgtcgata tcccagcttc cgctcaagga cgtctggtag gtggtgcggc
     2641 tccccccgct gctcgcgatc accggagtcc cggtccccgt cgtatcggaa gtaccggtgc
     2701 tggtgatgac cgtattgcgc gaacctcctc cggattgacg gaccgaagcg ttggcgctga
     2761 gcgcgggcag cagcgcaccg cgctcgatcc ccacttcggc acgcgccgcg cgataggccg
     2821 cttccgccga cctgaggttc tgattcgaga tatcaacctg cttttcaaga ccgtccagga
     2881 tcggatcgtt gtaaatcgcc caccagcttt cccagcccgc cgcctgttgc ggagaggcgg
     2941 gcatccatcc gcgattctcc ttgtagtctc caggctcgat cgcggaggga cgcatataat
     3001 cgggtcccag ttcgcagccc caaagcccta gcgcaagcgg cagcagcacg gcggcgcgcg
     3061 ttttcatggc gtggcctcgc gcggacggcc gaaaagaccg ccataggagc gtttccagag
     3121 accggcactc cacaggcgga aacgatccaa ataaagataa accaccggcg tcgtatacag
     3181 cgtcagcaac tggctcacca gcaatccccc gacaatggaa atccccagcg gctggcgcag
     3241 ttctgcgcca tccccggtga tgagcgcgag cggcagcgcg ccgaaaatcg cggcgagcgt
     3301 ggtcatcacg ataggccgga agcgcagcaa tgcggcgcgg tagatcgcct cgcgcgaatc
     3361 caacccttcg gtgcgctgcg cgacaagcgc gaaatcgatc atcatgatgg cgttcttctt
     3421 cacgatgccg atgagaagaa tgacgccgat cagcgcgatg aggctgaatt ccgcgccaaa
     3481 gatcagcagc gccagcaccg cgcccacgcc tgccgaagga agcgtcgaca ggatcgtgat
     3541 aggatggacg agactctcat agagaatccc cagcacgatg tagaccgtga caagcgctcc
     3601 gagaatcagc aatggctcat aggcgagcgc ctgctgaaac gaacgcgccg taccggcgaa
     3661 tgtcccgtgc accgagatcg gcacatgcaa cgtggccatg gtgcgttgga tggcggaaac
     3721 agcgtccgac agagactcgc cctcgggaag attaaaagaa aacgtcgtcg ccacgaacgg
     3781 accttgatga ttgaccgaca gcggcgtggt gcctgcgtca tagctggaaa atgccgcaag
     3841 cggaaccatg cgctccacgc gcgtactcac cgaagctcca gcggaggcgc cgccgcgcgc
     3901 ggtattggcg atctggttct gcgcttgatt ctggaaggcc gtcgcatcgg ccacgctgag
     3961 atcggtcgcg gcggccgcgg ccgtggtgct ggtcgcggcc ccgacggagc cggcggtatt
     4021 cgattctgcc ggggcggcgg ccgtggtgac gatccccccg ctcgcctgtg tgccggtgat
     4081 gtttcccgcc gtgctgacat aaacatccct gagcgtttcg gggttctgcc agaacgcggg
     4141 cgcgacttcc atcaccacat gatattgatt gaggtctttg tagatcgtcg acacctgccg
     4201 ctgaccgaat gcgtcataaa gcgtgttgtc gatttgtgtg gcggttacgc cgagccgcgc
     4261 tgccgtggac cggtcgatat gcaattcgac atcgagacca gcctcctgcc ggtcggaatt
     4321 gacgtcgagc agttccggca cgtcctgcag cgcttcggtg atgcgcggca cccaggtttc
     4381 aagctcggcc agcgtgtcgg attggatggt gtattgatat tgcgcgttgg cttgtcgccc
     4441 gcccacgcgc agatcctgga ccggagcgag aaacagccgc gcgcccgata cgttgccgag
     4501 gcgggtccgc agcctggcga tgacgtggtc ggtggtttcc ttacgctccg acaggggctt
     4561 cagattgacg aacacgtttc cggtgttggt ctgaccgccg cgccccgaga aaccgaccac
     4621 gctttgcacg gcagggtcct gatggatcgc attcacgaat tgatcgaact ttcccttcat
     4681 ggcctggaac gagatgctct gatcggcgcg gatgccgccc ccgatgcttc cggtatcctg
     4741 ctgcgggaaa aaacctttcg gtatgatggt aaaaagataa acattgagcc cgatcgtgag
     4801 agcgagaatg accatgatcg taccgggatt ggagagcgac caggagagcg accggtcata
     4861 ggcacgatgg acgcgcacga acacgcgctc ggagccgcgc atcagccagc cctgttcgcg
     4921 tttcgcgcgg cccatcccca gataggcgca catcatgggt gtggtggtga gcgaaagcac
     4981 cagagaaatc agaatcgcga tggacagcgt catcgcgaat tcgtggaaca gacggccgat
     5041 gaggccaccc atcagcagga tgggaaggaa taccgcgatc agtgagacgg tcatggaaag
     5101 gacggtgaag ccgacctcgc gcgcgccgag aagagcggct tgcatgcgcg gcatgccttc
     5161 ttccatgtgg cgtgagatgt tctcgagcac gaccacggca tcatcgacca cgaagccggt
     5221 ggcgaccgtg agcgccatca gcgacagatt gtcgaggctg aatccgagca ggtacatcac
     5281 tccgaacgtg ccgagaagcg atatcggcac cgccacgctg ggcaccagcg ccgcacgccc
     5341 attgcgcagg aacacgaaca ccacggcgat cacgagcagg accgcgatcc ccatcgtcgt
     5401 ctccacgtcg cggagcgaag cgcggatggt agtggtgcga tcgttgaccg gagtgatcgt
     5461 gatgctgttg ggaatcgagg cctggaggga cgggatcatc gccttgatgc ggtccacgac
     5521 gtcgatgatg ttcgcgcccg gctggcgatt caggatcaca agcaccgcgg gcttgccgtt
     5581 ggcgttgccc tgattgcgga tgtcttcgac tccatcgctg acctcggcga tatcggaaag
     5641 acgcacaggc gcgccatcgc gataggcgac gatgagggtc tgatactgtg ccgccttcgt
     5701 cgccgtgtcg ttgacataga tctggtagcg gcgttcgcgc tgttcgatag cgcccttggg
     5761 ggcattggcg ttagcggcgg aaagcgcggc acgcacgtct tccagtccga ttccatattt
     5821 gaagagcgcg cgcggattga gttcgacgcg cacggcaggc agtgagctgc cgccgatctg
     5881 cacctcgccc acgccatcca cctgcgacag tttctgctgc aaaacggtgg aggcggaatc
     5941 atagatctgg ccgggcgtca gcgtgtcaga ggtgagcgcc aggatcagga tcggcgcatc
     6001 ggccggattg accttgcggt aggtgggatt tgagcgcaac gccgccggaa gatcgacgcg
     6061 ggccgcattg atcgccgcct gcacatcgcg cgccgcgcca tcgatgtcgc gatcgaggct
     6121 gaattgcagc gtgatgcgca cctgaccgac agaactggag gatgtcattt cggtgacatc
     6181 ggcgatggtt ccaagatggc gctccagcgg cgtcgccaca ctggttgcca ccgtttcagg
     6241 actggcgccg ggaagctggg ccgtcacgga aatggtcggg taatcgacgt tgggcagcgg
     6301 cgagacgggc agcaggaaga acgccgcgcc gcccgcgatg gcgatccaga tcgtgagcag
     6361 cgtcgtcgcc acagggcgcg cgatgaagcg agccgagacg ttcatggcgc aacgcctctc
     6421 gcgccttcag gcgggacggc gttgcgccgg ctgcgcgagc gcgtcgccag ccggtcgaaa
     6481 tacagataga tcacgggtgt cgtgaaaagc gtgagaagct ggcttaccag caggccgccg
     6541 acgatggcga tgccgagcgg atggcgtaat tccgcgccgg tgccgccacc gagcataagg
     6601 ggaagagcgc cgaaaagcgc cgagacggtc gtcatcagga tggggcggaa gcgcagcagc
     6661 gccgcctgat ggatcgcctc gcgtggcggc tttttctctt cgcgttccgc gacgagagca
     6721 aagtcgatca tcatgatcgc attcttcttc acaatgccga tcagaaggat gatcccgatg
     6781 atcgagataa tatcgagatc gtgaccgctg acgatcaggg cgagaagcgc gcccaccccg
     6841 gccgacggca gggtcgaaag gatcgtgacc ggatggatga agctctcata cagcacaccc
     6901 agcacgatgt agacggtcac gatggccgcc acgatcagca acagttcatt gccgagcgcg
     6961 gattggaacg caagcgccgc gccctgaaag ctcgtggtga tttcgggcgg caatccgacc
     7021 tgggcttcaa ccgcccgcac cgcctcgacc gcggcgccga gcgagccccc cgcggccacg
     7081 tcgaatgaga tcatcgccgc cggaaattgt ccgatatgcc cgatctggag cggtccggtg
     7141 cgctcctcga tttttgcgat cgccgagagc ggaacctgcc ctcccgcgga agacggcaga
     7201 tagatcgttt ccagcgaagc cagcgagttc tggacgtcgg gatcggcttc gaggatcacg
     7261 cgatactggt tcgactgggt gaagattgtc gaaatgatgc gctggccgaa cgcgtcgtaa
     7321 agcgcgttgt cgatggttgc cgccgtgatg ccgaagcgcg cggccgtgtc gcggtccacc
     7381 gcgatatagg ccgagagtcc gcggtcgaga taatccgtgg aaatgttttc gatttccggg
     7441 gtccgcgcga gagcgtccat cagacgcggc acccattgcg ccagcgtggc gtcctgggcg
     7501 gcctgcaatg cgaactgata ttgcgcgcgt ccgaccgtgg actccacggt cagatcctga
     7561 accggctgca tataaagcgc gatgcccgag acgtcgtgcg tcgcgcgcga aatccggcgc
     7621 atgacgtccg tgaccgatgt gctgcgttcg ctcttggatt taagattaat caggaaacgc
     7681 ccgctgttca gcgtcgtgtt cgatccgtcc accccgatga aggacgacag gctgacaaca
     7741 tcggggtctt tgcggataat gtcggccagc gcctgctggc gttcggccat tgtggcaaag
     7801 gacacggtcg gggcggcgcg agtgacagcc tggatgaaac cggtgtcctg gaccgggaag
     7861 aaaccctttg gaatgccgat gtagagcacc accgtgacga cgagggtgac gatcgccacc
     7921 atgagcgtca aaaactgccg atccagcacc caatcgagga gacggccata ttgggcaatc
     7981 ccacggtcga accattcgcc cgccttgcgc gcgatccgcc cctgtttttg ctccgcttcc
     8041 ggatgcagca gcttcgcgca cagcatcggt acgagggtca gcgacaccac tgccgaaatg
     8101 agaatggtga tcgccagcgt caccgcgaat tcgcggaaca ggcgtcccac cacttcctgc
     8161 atgaaaagca gcggtatcag caccgcgatc agcgacaccg tcagcgacat gatcgtaaag
     8221 ccgatttcgc cggagccctt gagggcggct tccatcggtt tttcgcccat ttcgagatgg
     8281 cgcgagatgt tctcgatcat gacgatggcg tcgtcgacga cgaagccgga cgcgatggtg
     8341 agggccatca gcgtcagatt gttcagactg tatccgagca gatacatcgc cgcgaaggtc
     8401 ccaacgatgg aaagtgggac cgaaaggctg gggatgatcg tcgccggaac attgcgcagg
     8461 aacaggaaga tcaccagcac gaccagcgcg acggcgagcc cgagttcgaa ttccacatcg
     8521 gcgacggaag cgcggatcgt atttgtgcga tcggtgagca ccgtaacgtc gatggacgcg
     8581 ggaaggctgg tctttatgtc gggcaaaaga ttcttgacgc tgtcggccac cgctatgaca
     8641 ttggcgcctg gctggcgctg aacgttcagg agaatggccg gcgtcatatt catccaactc
     8701 gacagtttca cgttctccgc gctttcgatc acgtccgcga catcggaaag atgcaccgga
     8761 gcgccgttgc gataggccac gacgatgctg cgataatctt ccggggtctg aagctgatcg
     8821 ttggcattga tggtgtaggc gcgcgtggcg ccgtcgaaat tgcctttcgg cgcattggaa
     8881 ttcgcattgc cgatgatggt ccgcagatcg tcgatgttga ggccataagc ggcgagcgcc
     8941 tgaatgttgg cctgcacgcg gactgccggg cgctgcccgc cgccgatgct gaccaatcct
     9001 acgccaggga gttgagaaat cttctgcgcg atacgggtgt cggcgatgtc ctccaactgg
     9061 gtcatctgaa gcgacgcgga ggaaacggcc agggtgagaa tcggcgcatc cgcaggattc
     9121 accttggcgt aaaccggcgg cgcggggaga ttctgcggca acaggtttcc cgctgcgttg
     9181 atcgccgcct ggacctcctg ctcagcgatg tccaggttaa gcgaaagatc gaattgcagc
     9241 gtgagcacgg aagcccccgc cgaactggcg gaggacattt cgcgtagacc cggcatctgc
     9301 ccgaactggc gttcgagcgg tgccgtcaca gacgtggtca tcacttcggg actggcgccc
     9361 ggaaggaatg tctgcacctg gatcgtgggg taatcgactt cgggcagcgc cgaaagtggc
     9421 aggaagaaat accccaccaa tcccaccaga aggatcgcga ccatgaaaag cgaggtcgcg
     9481 atcggacgtt ggatgaaaat gcgcgatgga tttatcccgg tctcatccgg gccggtctca
     9541 ttcggattcg aatcggcaga catcatccgc cgccagggcc gccgcggccg cccgccgctc
     9601 cagcacctgc ggctccggct ccggcggctc cggcccccgc ggctccggca tcaggcgtcc
     9661 ccggtgcggc acccggagcg tcggctccct ggccaccccg tcgcccagcg cgaccttgcc
     9721 gccgcccgcc tgccccattt gcaggtgcgc ggccttgggt tgcagcggcc gcggggggct
     9781 gctcgcccgg cagcaacacc tttgcgccgt cgcgcaaacg atctgcgcca tccacgacaa
     9841 gagtatcccc ggccatgacc ccgcttgtca ccgcgacgcg gtcgtcctgc gtctggccca
     9901 cctgcacatt gcgcatggta acggtgtgat cggagttcac gacgaaaacg aaagtcccgg
     9961 acgcgccgtg ctggatggcc gaggcgggca ccaccatctg attgtgcagc gtgttgacga
    10021 gcaatcggat gttcacaaat tgattcggga acagttcgcc gctcttattg tcgtacatcg
    10081 cgcgcagctt caccgttccg gtcgtcgtat cgatctgatt gtcgatggtc gcgagtacgc
    10141 cggtggcgat tttcttgctt tgagcgcgat cgtaagcgtc ggatgtcagc ttggcgccct
    10201 gacgcatctg cgccaggaca tcgcttatgg catcctcggg aaccgcgaac accaccgaga
    10261 tcggttcaag ctgcgtcacc acggcaatac ccgttgcctg acccgcggtg agaagatttc
    10321 cgagatccac ctgtctcaac cccacgcggc cggtgatcgg cgatctgatc gtcgcgtaat
    10381 tcaggttgat ctgcgctgct tcgacattag cctggtccga tttgatcacg ccctgtagct
    10441 ggcgcacgag cgcggcctgt gtatcgactt gctgctgggc aatggaattc tgaccggcca
    10501 gcgtgcgata gcgctcaagg tcgacttgcg cattggcgag ttgcgcctga tcccgtgcaa
    10561 gctgaccatc cgcctgatcg agcgcggcct gatagggctt cggatcgatc tccgcgagca
    10621 cttgccctgc ccgtaccgtc tcgccttcgg tgaaatcgat tcggatgagt tggccgccaa
    10681 cttgtggctt cacagtcacg gtcgccagcg gcgtcacagt gcccagtgca ttcagcgtga
    10741 ccgtgatgtc accggaggtg actttcgcga taccgaccgg cgtggcctga ttggcgccgg
    10801 caaaagcatt gcgattgggg atcgcattgc gactcggacg gatggtccag acgagcagcg
    10861 cgacaagcgc gaccgcgatc gtgatgatga caatcttccg gccgcccggg atgcggtcga
    10921 tcccgcgcct cctcgtgatg tcatcagacg cggcatcgaa aggcttgcgg tcggtgcgtt
    10981 ggtccattgc tgcgtcgatc catcggcgtc acgcccacgc tttcacaatc gcgtaccgcg
    11041 tggcattaaa aagaggcaac ccaagcaa
//
LOCUS       DASYEG010000069        25013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1760452, whole genome shotgun sequence.
ACCESSION   DASYEG010000069 DASYEG010000000
VERSION     DASYEG010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 25013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25013
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760452"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VGM72_02620"
     CDS             complement(<1..521)
                     /locus_tag="VGM72_02620"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY4264184.1"
                     /translation="MKLDRIYTRGGDAGRTSLGDGARVPKHHLRVHAVGGLDEANAAI
                     GVARLHIEDAEVGNVLALAQNDLFDVGADMCRPEQPEANRQPLRVSAQQVLTVERQID
                     RFNEALSPLTSFVLPGGSPASAYLHLARTMVRRAERDMAELAAGEAVNAEALKYVNRL
                     SDLLFVLGRYTNQR"
     gene            complement(617..1396)
                     /locus_tag="VGM72_02625"
     CDS             complement(617..1396)
                     /locus_tag="VGM72_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4264185.1"
                     /translation="MTVLSASNLMMHRGGRPILRDVAFRAQSGEFVAIIGANGAGKST
                     LLASIAGLIVPDAGTIRLDDADLAGLTRVQIARRRAYLPQNPRCEWPLSVERLVALGL
                     TPVLPAFGGLPAEMQMRVDAMLDEWDLVFHRAQPATTLSGGELARAMLARALVGNPDI
                     LIADEPISGLDPRHALDCAGRLSALARGGKLVIASVHDLTLAARYATRICALDHGSVT
                     DDGPPEKILTPELLRSVFEVEACVSGAGARAFVDYLTPSAP"
     gene            complement(1393..2442)
                     /locus_tag="VGM72_02630"
     CDS             complement(1393..2442)
                     /locus_tag="VGM72_02630"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY4264186.1"
                     /translation="MTGVAEIASGDGRPGPGARPFTSGFPVAAPLIVILCVLAIVSLL
                     SGRVWLSPQEVWDGIFSSHSQLSSLIVIELRLPRTVLAIMVGATLGLCGAVLQGFTRN
                     PLAEPGLLGVSAGASFGAVIAIYFGISLISAVAIPVCGLIGALAAMSLTFAIGGGGGT
                     VSLVLAGAAVNAFLFALISLGLNLAPSIEAAYEIMTWLMGSLANRSWDHVLLVTPFIV
                     AGCAMLAFTGRSLDALSLGEIQAESLGVNLRRLSLIALIGTALAVGAATSVTGTIGFI
                     GLVGPHLVRPFVGYQPSRILFPAALAGAVLLLAADIATRWISIGPEMKLGVFTALVGT
                     PVFFWLVMRLRRVSP"
     gene            complement(2439..3302)
                     /locus_tag="VGM72_02635"
     CDS             complement(2439..3302)
                     /locus_tag="VGM72_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4264187.1"
                     /translation="MKRILAFFAAMAFGMPGVIVAAERPKPQRVMSLNLCTDQLVLQL
                     LPPERISSVTYISRIPGYSFLTAQARAVPVNDATAEEVLRDHPDLVIAGSTSTTPIRA
                     LLRKTGIPLLEVPAADNFEEIRNVTRLVAHAVGEDAAGETLIAHMDRTLGALAASAPT
                     KIVVAGWNGAGDVPAKGTLFDAILTAAGGVNVVAGINAHIVYGQYTGFDLEQLVAMRP
                     DLLAYGRSRAGALDLANQTLQHRIVRKLYAGRQITYPETLYNCGLPQSADAARDLRHA
                     MLAAMHKETAP"
     gene            complement(3299..5287)
                     /locus_tag="VGM72_02640"
     CDS             complement(3299..5287)
                     /locus_tag="VGM72_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4264188.1"
                     /translation="MPFDKNFLLKSAALILGPFWIGQPAVAQTAQAPSEIPESVLITG
                     SRGLFGLRTDLLGSSYTIIQPVDLELRQVEIVSDVLRDVPGVAVNRTGTVGGQTEVRI
                     RGAEGNHTLTLIDGIEASDPFAGEFDWAGLLADESAKIEVLRGQQSALYGSTAIGGVI
                     QYITLTGAEAPGVRAHLEGGSFDTLSGSARVAGVTGALDYALSGSLSRTGGIVGARNG
                     VRHDGAENEGFSGKFIYTVKDNLHITAVGRYSNVDAETTPQAFPFPADASFGLTYDTL
                     PGNPSVQVADSYTAKSLYGLLRGDLELMGGMWTHSLTLQGVSGDRKSYDDIRALSGDD
                     YGTRQKASYTTTINFATDNLAHALTFAADFKRETYQNKAVGAPPTLANDERHLDNAGF
                     VGQYDLRIGDDIGFGAAVRYDDNTHFQDATTYRVQASYRPMQDLRLRAAAGTGITNPT
                     NFELFGFDPATFIGNPNLKPERSEGWEAGLDYDLFAGMASVGATYFDSRLTDEIITAF
                     TPAFLSTPENLSTKSTQQGVELWGSARISEQWRIDATWTNLHALEGSPAVQEVRRPPN
                     IASLNVSWRAADDRFGANLTLRYNGEQVDFQFTPAATLRVPMKAYTLVNLGADYRLDD
                     NWQIYGRVENLFDEKYEEVFAFPSPGIAVYGGLRFRMR"
     regulatory      complement(5478..5695)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5910..6404)
                     /locus_tag="VGM72_02645"
     CDS             complement(5910..6404)
                     /locus_tag="VGM72_02645"
                     /inference="COORDINATES: protein motif:HMM:NF022893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3016 domain-containing protein"
                     /protein_id="HEY4264189.1"
                     /translation="MRTLVLFAAAIVTGTWPFQASAAVIVNFIQPEHYTDAGPQGQDR
                     TRNLNEIERTFQKLGEHYLSADQILTIDVLDVDLAGRLEPWHANAYDVRYLRDITWPR
                     MKLRDTLETPSQPPQSAEEILSDPSYLESPNTRFSGEPLAYDKAMIERWFHARFVEHR
                     PPHH"
     gene            complement(6833..6967)
                     /locus_tag="VGM72_02650"
     CDS             complement(6833..6967)
                     /locus_tag="VGM72_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264190.1"
                     /translation="MTLRGRILRYRELAATLYNHDLVAEVEALAAELEEEAAKLVGGA
                     "
     gene            complement(7157..8002)
                     /gene="rlmJ"
                     /locus_tag="VGM72_02655"
     CDS             complement(7157..8002)
                     /gene="rlmJ"
                     /locus_tag="VGM72_02655"
                     /inference="COORDINATES: protein motif:HMM:NF016278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEY4264191.1"
                     /translation="MNYRHAFHAGNFADCLKHAALVAVLLHLRKKQTPFAVIDTHGGR
                     GLYDIAGEEAKKTGEAEAGIKRLLRAPSVPGVLDLYRECVRGFGEGAYPGSPLIASKF
                     LRPQDRLIAIEKHPEEQAALSAALAHEKRVRVVQGDYVRELPRFLPPPERRGVVLIDP
                     PYEAEDEFARATRTLLAAHARFATGIFLFWYPAKERALVGASAGELLNAGIASLLRVE
                     LDVGAPEEKREETRERLSAAGLLIVNPPFGFAGEMQRIMPFLERHLAQGTGARGLVRS
                     FTSTQ"
     gene            complement(8035..8604)
                     /gene="efp"
                     /locus_tag="VGM72_02660"
     CDS             complement(8035..8604)
                     /gene="efp"
                     /locus_tag="VGM72_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY4264192.1"
                     /translation="MVSVIASSIRKGNVVEKDGKLYAVIRAENIHPGKGTPVTHLEMR
                     RISDGVKTVERYRTTDSVEKAFIDQGTFNYLYKDGEHYVFMDPVNFEQIHVSADILGD
                     SAVYLQENMEVSMSQYEGVPISMELPARVVLEVVETEPVVKGQTASSSFKPAVLSNGA
                     RTMVPPHINVGTRVVVQTEDGAYLERAKE"
     gene            8749..9792
                     /gene="epmA"
                     /locus_tag="VGM72_02665"
     CDS             8749..9792
                     /gene="epmA"
                     /locus_tag="VGM72_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HEY4264193.1"
                     /translation="MTGPSPWWDRGVHADRRPLLLARGRVRDALRHYFAGEGFIEVEC
                     GALQTSPGNEAHLHAFSTERIGTDGALRTLYLHTSPEFACKKLLAAGERRIFTLAPVY
                     RNRERGDLNASEFTMLEWYRAEEPYACVIVDCLAILKQAAGAVENTLFRFRGIECDVS
                     LKPERISVCDAFRLYAGIDLLATLDTEGAADRNALAGAAETAGVKIAADDSWSDIFSR
                     ILTERIEPRLGLGAPTVLDRYPRVEAALARAASDDPRVAERFELYVCGVELANGFGEL
                     TDTVEQRRRFEAEMAEKERIYGEPYPIDEDFLAALGHMPEACGVALGFDRLVMLATGA
                     QRIGSVMWTPPVA"
     gene            9853..10899
                     /locus_tag="VGM72_02670"
     CDS             9853..10899
                     /locus_tag="VGM72_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-2,3-aminomutase-like protein"
                     /protein_id="HEY4264194.1"
                     /translation="MLDRRRTLRTPEELAAAGLIEACQTGALAPVAERYAIAITPAIA
                     DLIDTADPNDPIARQFVPSPAEGDTHPQERADPIGDERHSPVAGIVHRYPDRVLLKLT
                     HVCPVYCRFCFRREMVGPGENQVLGADELDAALAYIAAHAEIWEVILTGGDPFMLSPR
                     RLAQVTARLSAIAHVKILRWHTRMLSADPERVTPELVRAIRTDKTTYVVAHVNHPREL
                     TDAARRAASRLVEAGIPLLSQSVLLKGVNDDLATLEALMRALVESRIQPYYLHHMDLA
                     PGTAHFRTSIADGQALVRELRARISGLCQPHYVLDIPGGYAKALLNSCDAERAGPLHR
                     IRDGEGKLHDYPFA"
     gene            11037..11339
                     /locus_tag="VGM72_02675"
     CDS             11037..11339
                     /locus_tag="VGM72_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264195.1"
                     /translation="MNKLILPLLLATAAFATPASANWFHNPYQGINRNIGSAPNPTPA
                     DIQEMRLPQATKVETPMLNYTVVEPAKDPNKTASAATSANAPAQGAGTVPAAAPSR"
     gene            11514..12041
                     /locus_tag="VGM72_02680"
     CDS             11514..12041
                     /locus_tag="VGM72_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264196.1"
                     /translation="MKTAKTLMSLLLLSTVSVAPAYANWFSNPRTNTMMNIGSAPNPT
                     PADLRSIGDSTAVALNGNAGSREPVERVAYVAADRSHAVRREQLTRLEGKAVFGAHGV
                     RLGSILTVNLAARMAEVQTPSGIAVAMPVGLLRDRGNHVAAPTISRADMTAMAKTQTG
                     HTIAINIDRRRPSRG"
     gene            12428..14263
                     /locus_tag="VGM72_02685"
     CDS             12428..14263
                     /locus_tag="VGM72_02685"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4264197.1"
                     /translation="MDVDTGQVSDRISGLPPTNINDPSYFHKVVDCQWACPAHTPVPE
                     YIRLIAAGRYSDAYMVNWHSNVFPGVLGRTCDRPCEPACRRGRVEEGKAAQPVAICRL
                     KRVAADHKDDIRSRLPVAAIAKNGKRIALIGAGPASLTVARDLLPLGYHCVVYDADAS
                     AGGMMRTQIPKFRLPETVLDEEVNYILGLEPELRLGKRIESMKAVLEEGYDAIFVGCG
                     APRGRELEVPGRKEAAANIHIGIDWLSSVSFGHIDKIGKRVIVLGGGNTAMDCCRTSR
                     RLGGEDVKVVVRSGFEEMKASPWEKEDAMNEGIPISNYLVPKEFTHDDGRLTGVIFEK
                     VKAEYDPKGRRTLVPSGEPDVHMECDDVLVAVGQENAFPWIERDLGIEFDRWDMPKVN
                     TVTMQSTHPKIFFGGDAAFGPKNIIWAVAHGHEAAVSIDLLCSGNEVHHRPSPLTNLS
                     SQKMGIHEWSYDNDISADKRYKVPHAPPALTLKDVRMEVELGFDVKQAFMEAERCLNC
                     DVQTVFEGPKCIECDACVDICPMDCITFTENGPEVELRQRLIAPSSHPDTQDLYISET
                     LKTGRIMAKDEDLCLHCGLCAERCPTGAWDMQKFLFNTAKAGCHV"
     gene            14256..16085
                     /locus_tag="VGM72_02690"
     CDS             14256..16085
                     /locus_tag="VGM72_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEY4264198.1"
                     /translation="MSKPIKSINDFVVKFANVNGSGSASANELFARAILRMGVPIATR
                     NIFPSNIQGLPTWFEVRVSAKGWGGRRGGCDLMVAMNPQTWDKDLASIDAGGYLFYDS
                     SKPLPESKFRSDVNIVGMPLTEICNRTYHDPRQRQLFKNIIYIGALAAILEIDLAVVE
                     TLITEQYGAKKPLLESNIQAVRMGHAYARENLSGICGLHVARANAVGNRIFVNGNDAA
                     ALGCVYGGATVAAWYPITPSTSLAESFMKYCRKLRVDPETKKNKFAIIQSEDEISAIG
                     SVVGAGWNGARAFTATAGPGISLMQEFLGLAYFAEVPAVIFDVQRGGPSTGMPTRTQQ
                     SDLLVCAYASHGDTKHVLLFPQDPGELFEMAAQAFDLAERLQTPIFVMEDLDIGMNDW
                     LCEPFAWDDARKYDRGKIMTAEDLDAGKTFGRYLDVDGDAIPFRTLPGTHATKGAYFT
                     RGTSRDRFAKYSEEGSVYVDNMQRLVRKFETAKSLVPAPILHKSRETARHGVIYFGST
                     SAAMAEALVALESEGLHLDILRIRAFPFPEAVVDFINAYDQVFLIEQNRDAQMRMLLV
                     NECAIDPARIISVLHYDGTPITARFITAAIAERLQMKAKEAAE"
     gene            16082..17137
                     /locus_tag="VGM72_02695"
     CDS             16082..17137
                     /locus_tag="VGM72_02695"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEY4264199.1"
                     /translation="MTYIAKPKFHHPALPKNSLGFTHRDYEGSISTLCAGCGHDSISA
                     AVIQACFELSIEPHRLAKLSGIGCSSKTPDYFLGQSHGFNTTHGRMPSVLTGANLANK
                     DLLYLGVSGDGDSASIGLGQFVHCVRRGVNMLYITENNGVYGLTKGQFSATADKGSKS
                     KAGGANNDSPLDLVGMALLTGATFVARSFSGDKKQLVPLIMAGLSHPGPAFIDVISPC
                     VAFNNHPGSTKSFDFVRAHNEAVNRLDYMDPREPIIVDYAPGTVSEVTLHDGSTLRLA
                     KLHPEYDPHDRVGAMTYMQSRAAAGELVTGLIYMDPQPRDMHTMLNTVDAPLNRLSEK
                     ELCPGSAALEKINAGLR"
     gene            17142..18086
                     /locus_tag="VGM72_02700"
     CDS             17142..18086
                     /locus_tag="VGM72_02700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4264200.1"
                     /translation="MARIIFLLPFAKHEITGGIKTTYRHAELLAELGFDAFVYQPGGP
                     PAWFETRAKLLTQLVPDPGDILVFPESLNGPLMDMMRLEARKVLFCQAHYYTLFNAVA
                     PETYRDLGFERAACQSTIAKGFLERVLGLSNIAVLPCFIDGELFLPRAKSMQIAAIPK
                     KLPREAAAIQKIFHLKYPGLAATRWEIIEGRSERETAEIFGRSAIVLSLPFLESFGLV
                     PLEAMASGAIVVGFTGYGAQEYANSQNGFWFPPDHLEEVADALAQVITGLQTNDPQIS
                     KIRETGFATAAVYSKDQTRAALKSFYGSLMQDRAGASA"
     gene            18083..19090
                     /locus_tag="VGM72_02705"
     CDS             18083..19090
                     /locus_tag="VGM72_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY4264201.1"
                     /translation="MKRDSSTPAGTVTRQIVDSEVLKTNPLGDSTSRIVDVYVPAGHD
                     GHGLPLLVDLVGFTAGGPAHTNWRNFTENIPEQADRLIATGAMPPCVIAFPDCFTRLG
                     GNQYINSAGIGRWADFLLQEAVPFVERAFGCGGAGKRGLFGKSSGGYGAIVHALLYPD
                     FWAASACHSGDMGFELLNIPEMPRVLRALAKQGGTKGWLEAFFAKRKVKDDDIHVLMM
                     LAMCATYDPDPRAFMGVRLPVDIETCTFIPERWANWMKWDPLNLVEIHHAGLKRLKAL
                     FIDCGDIDQYNLVYGARRMHRALQTYGVAHIYEEFPDDHSGIDYRMDRSLPVLAKALA
                     R"
     gene            19198..19851
                     /locus_tag="VGM72_02710"
     CDS             19198..19851
                     /locus_tag="VGM72_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264202.1"
                     /translation="MGRGFLPLVAVLLFAACNPAGATNFTAEVVDQDGHPVVNAVVSL
                     ISETKASMPAALTHLATEKTIDQRNETFLPLVTIVPKGGHVVFANNDQTTHQVYSFSA
                     IKQFEITLARGEKSPPVAFEKAGIAALGCNIHDHMIAYAFVAESPWTALTGADGRVTI
                     ADVPAGNYQAQVWHPRFPPGREPPSLRAVLSGDTTHLSVSVKLPYAATASRGHAGSY"
     gene            19861..21681
                     /locus_tag="VGM72_02715"
     CDS             19861..21681
                     /locus_tag="VGM72_02715"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4264203.1"
                     /translation="MSFRKRLALFLMVTLIGVQVITAIFAYGVIRGNLVEQAKNELKS
                     TASVFTRQLNVLSERVSDDVEVLSLDYALRKAVAEHDQATVLSALRNHGNRVGATRMM
                     LVGLDGKITADTTDERAVGTAFQFSDLIETASANDQGTSLAVLDGAIYWIVVVPVRAP
                     VPIAFIAACVPVNDALLEKLRQLSSVPQSLALATTDARGNWAVVSKTAGYAPTVRLTS
                     QTASIPQDSIIATEQKDDRLAMMARLATSQSSRPVVAVLDYSLEEALNAYRVVITPML
                     LVLGGALLVALVGAMLIAHGVSRPLETLAATARRIAKGDYTPMPAIGRSDEIGELLLA
                     LNNMTQSIAERETALKSAVASLELARNQAVKASEAKSQFLSNMSHELRTPLNAIIGFS
                     EMIHRQVLGPIPVQRYVEYAHHVFDSGRRLLFQVEEMLDLSDAESDKLSLARISLKPG
                     SLLSASLETLAPFAAKSGVRLDICGEMGSWPLIAADAAKLEQSLTNVVHNAIKFTPAG
                     GLVTVSGGCAGSMLKITVSDTGVGIRSEDLDLVVRPFHRRKSAFDAVYQGAGLGLPFA
                     KTIIDLHGGTLAIQSTQGIGTTVTMELPLAMDATALNHAA"
     gene            21687..22901
                     /locus_tag="VGM72_02720"
     CDS             21687..22901
                     /locus_tag="VGM72_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264204.1"
                     /translation="MRKLLAALFLIGSCSAAFAVDFHFDGYVDLRLIVPSNQESWQDG
                     QLGKLRYGAEDGKPELRMAEAVGQGVALITPELMALAVVRIEPEQRTFLDFLEAYARY
                     RPVSTTPWRWSVKAGAFFPPISLENTEIGWTSPWTLTPSAINTWVGEELRTIGVEGAL
                     EWRSEERTITAMASVFGWNDPAGALIADRGWALHDRVTGLIDRPREPDVIAAKIGVSV
                     PLYTYEALEIDDRPGWYAAASWDEAGLGFLNLIYYNNEADPAAVRRDTIAWRTDFWDV
                     GARTQFGDVTLMAQATTGQTMIVPNAFFFSDTTFKSAYLLAGWTIVQDWRVAARADVF
                     STDEKNPFPGNQSEHGNALTFAVNYLPYDWLRLTAEAIRVDSTRNQRTLAGLNPRAVE
                     NQLQLSAKFYLP"
     gene            complement(22889..24373)
                     /locus_tag="VGM72_02725"
     CDS             complement(22889..24373)
                     /locus_tag="VGM72_02725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY4264205.1"
                     /translation="MRSISHENPIRAVVLCVLALILVRGIVAGLTPLSYDEAYYWLWS
                     KHLAAGYLDHPPLIAFTIAAGTALFGDTSWGVRFVPWLLSVLASWAVWRSGRLILKSE
                     YGAVLAALFFNVMPMIGVEALVATPDSPQIAAAAFLLLTLAKVAETGNGLWWIAAGVA
                     AGFALLSKYTGFFLGVGILAWLAIVPRERRWFLSFWPYLGAAIGLLMFVPVLLWNAEH
                     DWISFAKQFGRLDEGGFTLRFLVEFLGGQLALATPFIAILGVAGVVMIFRSREGMRSE
                     PALLFAMIAPPVIYFAWHALHDRVQGNWPSFLYPAFVLAAAATCLRVEQKGSPWFLRL
                     SRRIAVPFAGAMLAVVYAQALWGIIPRVRDPVSRLLAFGIDPVANQIEALRNRTHARA
                     VLTTGYALTGWLSFYLPSHPPVVQVNERVRYLDEPEPPRALFEGPLLYVTETRNDQSQ
                     LLAAHFARIMPLAHISRVRNGATIDEYAIYGVSGLKGYPLDQGR"
     gene            24646..>25013
                     /locus_tag="VGM72_02730"
     CDS             24646..>25013
                     /locus_tag="VGM72_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEY4264206.1"
                     /translation="MQENEISFKPKKSVALSGVVAGNTALSTVGHSGNDLHYRGYDIL
                     DIANRCEFEEIAHLLVHERLPGAGELAAYKAKLRAMRGIPANVRTVLEAMPAAAHPMD
                     VMRTAVSALGCIAPEKDGHNP"
BASE COUNT         5066 a         7864 c         7420 g         4663 t
ORIGIN      
        1 cgctggttcg tatagcggcc cagcacgaac agcaaatccg acagccggtt gacgtatttc
       61 agcgcctcgg cgttgaccgc ttcgcccgcc gccagctcag ccatgtcgcg ttcggcccgg
      121 cggaccattg tgcgcgcaag atgcagatag gccgatgccg ggctgccacc cggaagcacg
      181 aaactcgtga ggggactgag ggcctcgttg aaacgatcga tctgcctctc gaccgtcagc
      241 acctgctgcg cgctcacccg caatggctgc ctgttcgcct ccggctgttc ggggcggcac
      301 atgtccgcgc cgacgtcgaa gagatcgttc tgggcaagcg caagcacatt gcccacctcg
      361 gcatcttcga tatgcagccg tgcgacgccg atcgccgcgt tcgcctcgtc gagaccgcca
      421 acggcatgca cgcgcaaatg atgtttgggc acacgtgcgc catcgccgag tgaggtgcgg
      481 ccggcatcgc cgccgcgggt ataaatacga tcgagtttca cgatgctgtc tttcgcagcc
      541 gaagaggaac cgtccgcacc tggaagtcac aatttatcat cacgtttgac ccatttccaa
      601 tttttcctac cgggcctcat ggcgccgacg gcgtgagata atcgacgaaa gcgcgcgctc
      661 ctgcgcccga gacacaggct tcaacctcga aaacggagcg cagcaattcc ggcgtcagga
      721 ttttttccgg cggtccatcg tccgtgacgc tgccatggtc cagcgcgcag atgcgcgtgg
      781 cgtaacgcgc cgccagtgtg aggtcgtgga cggaggcaat cacgagtttt cccccgcgcg
      841 ccagagcgct cagccggcct gcgcagtcaa gggcgtggcg cgggtcgagt ccggaaatcg
      901 gctcatcggc gatcaatatg tccggatttc caaccagggc gcgcgccagc attgctcgcg
      961 ccagctcgcc cccggataat gtcgtcgcgg gctgcgcacg atggaacacg agatcccatt
     1021 catcgagcat tgcatcgaca cgcatctgca tctccgcggg caaaccgcca aatgccggaa
     1081 gtaccggagt caatccgagc gcgaccagcc tttcgacgga gagcggccat tcgcagcgtg
     1141 gattttgcgg caaataggcc cggcgccgcg cgatctggac acgcgtgaga cccgcaagat
     1201 cggcatcgtc cagccttatc gtgccggcat ccggcacaat aaggccggcg atcgatgcaa
     1261 gcagcgtcga ttttccggca ccgttggcgc cgataattgc gacgaactct ccactctgcg
     1321 cccgaaacgc gacatcgcgc aggataggcc tgccgccgcg atgcatcatc agatttgagg
     1381 cagaaagcac ggtcatggcg acacccgccg cagacgcatc acgagccaaa agaagaccgg
     1441 cgttcccacg agcgcggtga aaacgccaag cttcatctcc ggaccaatgc tgatccatct
     1501 cgtggcgatg tccgcagcca gcagaagaac cgcgcccgcc agcgccgcgg gaaaaagaat
     1561 gcggctgggc tgatagccca cgaacgggcg caccaggtgc gggccgacca ggccgatgaa
     1621 cccgatggtg ccggtcaccg aggtcgccgc gcccaccgcc agcgccgttc cgatcagcgc
     1681 aataaggctc agccggcgca gattgacgcc caggctctcg gcttgaatct cgcccagcga
     1741 cagcgcatcg agcgagcgcc cggtaaatgc cagcatcgca caccccgcca cgatgaacgg
     1801 cgtgacaagc agcacatgat cccaactgcg attggcaagc gaacccatca gccaggtcat
     1861 gatctcatag gctgcctcga tgctcggcgc caaattcaat ccgagcgaaa tgagcgcgaa
     1921 cagaaatgca ttcaccgccg cgcccgcgag aaccagactc accgtgccgc cgccgccgcc
     1981 gattgcaaat gtcagcgaca tcgccgcaag cgcaccgatc aggccgcaaa ccggaatcgc
     2041 aacggccgaa atcagggata ttccaaaata gatcgcgatc acggccccga aggatgcgcc
     2101 cgccgagacg ccaaggaggc cgggttcggc caaggggttg cgcgtgaagc cttgcaggac
     2161 ggcgccgcaa aggccgagcg tcgcacccac catgatggca agcacggtac gcggcaaacg
     2221 caactcgatc acgatgagcg aagacaattg cgaatgcgaa gagaaaatcc cgtcccagac
     2281 ctcctgcgga ctgagccaga cccgccccga gagcagcgag acgatcgcca gcacgcagag
     2341 gatgacgatc aacggcgcgg cgaccggaaa accggacgtg aacggccggg ccccgggtcc
     2401 ggggcgtcca tcgccggatg caatctccgc caccccggtc atggcgccgt ttccttatgc
     2461 atggcagcaa gcatcgcatg gcgaagatcg cgagccgcat cggccgattg cggcaaaccg
     2521 caattataaa gcgtttcggg ataggtgatc tgacgcccgg cataaagctt acgcacgatc
     2581 cggtgctgaa gcgtttgatt ggccagatcg agagccccgg cccgcgaccg gccataggcg
     2641 agaaggtcgg gacgcattgc aacaagttgt tcgaggtcga agccggtgta ttggccatag
     2701 acgatgtggg cgttgatacc cgccaccaca ttcacgcctc ccgcagcggt gaggatcgca
     2761 tcgaacaagg ttcccttggc cggaacatcg cccgcgccgt tccagccggc cacaacgatc
     2821 ttcgtaggcg cgctcgccgc cagcgcgcca agcgtcctat ccatatgcgc aatcaatgtt
     2881 tcccctgccg catcttcgcc aacggcgtga gcgacaagcc gcgtgacgtt ccggatttct
     2941 tcgaaattat cggccgccgg cacttcgaga agcggtatcc cggttttccg caacaaggca
     3001 cggattggcg ttgtcgatgt gctgcctgcg atcaccagat ccggatggtc ccgcagcact
     3061 tcctccgcgg tcgcatcgtt gaccggcacc gcgcgggctt gagcggtcag aaaggaatag
     3121 cccggaatgc gcgaaatata ggtaaccgaa ctgatgcgct cgggcggaag caattgcagg
     3181 accaactgat ccgtacacaa attgagcgac atcacacgtt gaggcttggg tctctccgca
     3241 gccacgatga cgcccggcat gccgaacgcc atcgctgcaa aaaaggcaag gatgcgtttc
     3301 atctcatccg aaaccgcaat ccgccataga cggcaatgcc cggcgagggg aaggcgaaaa
     3361 cttcttcgta tttctcatcg aagagattct ccacccgtcc gtagatttgc caattgtcgt
     3421 caaggcgata atcggcgccc agattgacca gcgtataggc cttcatcggc acccgtagcg
     3481 tggccgccgg tgtgaactgg aaatccacct gctcaccgtt ataacgcaac gtcagattgg
     3541 cgccaaaccg gtcgtccgcc gcccgccatg acacgttcag acttgcgata ttcggcggac
     3601 ggcgcacttc ctgcacggcg ggactgccct ccaacgcgtg cagattggtc caggttgcat
     3661 cgatgcgcca ctgttcgctg atacgcgcac tcccccagag ttcgacgcct tgctgcgtgg
     3721 atttcgtgct gagattttcc ggagtggaaa gaaacgccgg cgtgaacgcg gtgatgatct
     3781 catccgtgag ccggctgtcg aaataggtgg ccccgaccga ggccatgccc gcgaacagat
     3841 cgtaatccaa gcccgcttcc cacccttcgg agcgctccgg cttgagattg ggattgccga
     3901 tgaacgtcgc gggatcaaat ccgaacagct cgaaattggt gggattggtg atacccgtgc
     3961 ctgccgccgc gcgcagacgc aaatcctgca tcgggcgata gctcgcctgg acccgatacg
     4021 tcgtggcgtc ctggaaatgt gtgttgtcgt cgtagcgcac cgcggcgcca aagccgatat
     4081 catccccgat ccgaagatcg tactggccga cgaagccggc attgtcgaga tggcgttcgt
     4141 cattggcgag cgtcggtgga gcaccgacgg ccttattctg ataggtctct cgcttgaaat
     4201 cggccgcgaa cgtcaacgcg tgcgcgagat tgtcagtcgc gaaattgatc gtcgtcgtgt
     4261 aggacgcttt ttgacgggtt ccgtaatcgt ccccagagag cgcgcggatg tcgtcgtagc
     4321 ttttgcggtc gccggacacg ccctggaggg tcagcgaatg cgtccacatc cctcccatca
     4381 gttcgagatc gccgcgcagc aaaccgtaga gcgattttgc tgtgtagctg tcggcgacct
     4441 gaacggacgg gttgcccgga agcgtgtcgt aggtgagccc aaaactcgcg tctgccggga
     4501 agggaaatgc ctgcggcgtc gtctcggcat cgacattgct gtagcgccca accgcggtga
     4561 tatgaagatt atccttcacg gtatagatga acttgccgga gaaaccttca ttctccgcac
     4621 catcatgccg gacgccgttg cgcgcgccga cgatgccgcc ggttcgggac agagagccgc
     4681 tgagcgcgta gtccaatgcg cccgtcactc ctgccacgcg cgccgatccc gagagcgtgt
     4741 cgaaggaacc gccttccaga tgcgcgcgca cgcccggcgc ctcggccccc gtcaaggtga
     4801 tgtattggat gacgccgccg atcgcggtcg agccatacag cgcgctttgc tggccgcgaa
     4861 gaacttcgat cttggccgat tcatccgcaa gcaaaccggc ccaatcgaat tctcccgcga
     4921 agggatcgct cgcctcgatg ccgtcgatca gcgtcagcgt atgatttcct tccgcacctc
     4981 ggatgcggac ttcggtctgg ccgccgaccg tgcccgtgcg gttgaccgcg actccaggga
     5041 cgtcgcgaag cacatccgac acgatctcga cttggcgcag ttccaaatcg acgggctgga
     5101 taatcgtata agacgacccg agaagatcgg tgcgcagacc gaaaagtcca cgcgaaccgg
     5161 tgataaggac cgattccggg atctccgagg gggcctgcgc cgtctgagcg acggcgggct
     5221 gcccaatcca aaagggcccc aaaatcaaag cagcggattt tagaagaaaa ttcttgtcga
     5281 acggcataat ccccccaaat ggggccttcg ccccggttgc atgcgcgact gacccggctg
     5341 ccgggccatt ggtccggcag ccctaagacg gaacgccaaa cgcgcacggc ttcgcgggcg
     5401 cgtccaacgc tccatgcggc ttgtggccgc cctgtcgctc ggcgggataa tccgtgcact
     5461 gaactcacct ggttcaggca tgagcgacgc gcaccggccg gtctactggc ttcgcgggtc
     5521 aacgcgtcat gcccgccttc ccaggaaaaa ttcccagtgg ctgcgaccca ttacggggcc
     5581 gcgtgggcat acgctcaccg cttacagttg cagggacagc ccagtttcag gccttgcggc
     5641 ctttagcttg agttcccgtg gagcccttgc gggcaccggc gcgcatggat gaggctctga
     5701 gagactcatc gggcatcagt cttctcccct attgcggatt cgtcaactct cacctgagcg
     5761 agaattttcc acgagggaac atctacggtg tcgcgtatca gggttgaaac cggctgccgc
     5821 agcttccgtc cgcgacgtct cacactcgcg gagcggcacg ccggtttctt ctcttgatta
     5881 ttgctgtttt tggtggatga cgccgggcat caatgatgcg gagggcgatg ctctacgaaa
     5941 cgcgcgtgga accagcgctc gatcatcgcc ttgtcatagg ccaaaggctc gccggaaaag
     6001 cgcgtattgg gagactccag ataggatgga tcggacaata tttcctcggc gctctgcggg
     6061 ggttgcgacg gcgtctccag cgtgtcgcgc aatttcatcc gcggccaggt gatgtcgcgc
     6121 agatagcgca catcatatgc gttcgcgtgc cagggttcca atcggcccgc caagtcgaca
     6181 tccagaacat cgatggtgag aatctgatcg gcggacagat aatgttcgcc gagcttttga
     6241 aacgtccgtt cgatttcatt gagattgcgc gtgcggtcct gcccctgggg accggcatcg
     6301 gtataatgtt ccggttggat gaaattcact atcaccgcgg cggaagcttg aaacggccac
     6361 gtcccggtta cgatcgccgc tgcgaagaga acaagggttc gcattgcgtc tccacccttt
     6421 ggattcgatc ggctgcctga cttaaaaata tcgatactga cacttgcatg ccataacgat
     6481 tccaacgggg ctttgttctg ttaccgcggg ttgaaacgtg aggcgtcgcg gctatcaact
     6541 ctatgtgggc ggggaaaaag tgtcggaacc ctcgcagcaa agacccgtta ccggcgcatg
     6601 tcgaggcatc ttcaccggga tgatccctgc cccgaatgtg gtgagcgttt gatccacagc
     6661 ggtgcaatct gggctttttt catgcgctcg tcatttctga aatgccccaa ctgcaaactg
     6721 attgcgtgga aacatgcaac cgcctatggc cgcagacggg agcagcatag ggaagcccgg
     6781 gcttggcatt taggcgcgtt tctcaggtcg ccgtttttca ggcccccgtt tttcaggcgc
     6841 cgccgacgag cttagcggcc tcttcttcga gctcggcggc taaagcttcc acttcagcga
     6901 caagatcgtg attgtagagg gtggcggcaa gttcccggta gcgcaggatg cgcccgcgca
     6961 aggtcaaaag tttttctgac gcctctgttt tgacaatcgg cgcggcggcg tcgcattgcg
     7021 ccgtatctcc accggaatca aacctttcag gcctaaaaca aagcataggc gcctccagcc
     7081 gagtcgcccc cttcccatga ccgtgagcct aggctcggct ggtaaacaaa tggtaaatta
     7141 gagaccgttt ccccccttat tgcgtgctcg tgaagcttcg caccagtccc cgcgctccgg
     7201 tgccttgggc gagatgacgt tcgagaaacg gcatgatccg ctgcatctcg ccggcaaagc
     7261 cgaagggcgg attgacgatc agcaatcccg ccgccgacag ccgttcccgt gtttcttctc
     7321 tcttttcctc cggcgcgccg acatcgagtt ccaccctcaa gagggacgcg atgcccgcgt
     7381 tcagcaattc gccggccgat gcccccacga gcgcgcgttc ctttgccgga taccagaaaa
     7441 ggaaaatccc cgtcgcgaaa cgggcgtgcg ccgcgagcag cgtacgtgtc gcccgggcaa
     7501 attcatcctc cgcctcatag ggcggatcga tcagcacgac accgcgccgt tccggaggcg
     7561 gcaaaaaacg cggcagttcg cgcacatagt cgccttgcac gacgcgaaca cgcttttcat
     7621 gcgcaagcgc ggccgaaagc gcggcctgtt cctcggggtg tttttcgatc gcgatcagcc
     7681 ggtcctgcgg gcgcaggaat ttggacgcga tgagaggcga gccgggatag gcgccttccc
     7741 cgaagccgcg cacgcattcg cgatagagat ccagcacacc gggcacggat ggcgcgcgca
     7801 acaaccgttt tatgccggct tccgcctcgc cggtcttttt cgcttcttcg cccgcgatgt
     7861 cataaagccc acgtccgcca tgggtgtcga tcaccgcaaa gggcgtctgc ttcttgcgca
     7921 aatgcagcaa cacggcgacg agtgccgcat gtttcaggca atcggcgaaa ttcccggcat
     7981 gaaaggcgtg gcggtaattc atgggcgggc gcgagagaag gccgcgcggg tatcctactc
     8041 cttggcgcgc tccagatagg caccgtcttc ggtctggacc acgacgcgcg tgcccacatt
     8101 gatatggggc ggcaccatag tgcgcgcgcc attggaaagc accgccggct tgaacgaaga
     8161 cgatgccgtc tggcccttca ccacgggctc ggtctccaca acctccagca ccacgcgcgc
     8221 cggcaattcc atggaaatcg gcacgccctc gtactggctc atgctcactt ccatgttctc
     8281 ttgcagatag accgccgagt ctccaaggat atccgcagac acatggatct gctcaaaatt
     8341 caccggatcc atgaacacgt aatgctcgcc gtccttgtag agataattga acgtaccctg
     8401 gtcgataaag gccttctcaa ccgaatccgt cgtgcggtag cgttccaccg tcttcacgcc
     8461 gtcggagatg cggcgcattt ccagatgcgt caccggcgtg cccttgccag gatggatgtt
     8521 ttcggcgcgt atgacggcat aaagcttgcc gtccttctcc acgacgttgc ccttgcgaat
     8581 actgctggcg atgactgaga ccacgcgatg cctctttgat tgtgcccgga ctaggttttt
     8641 gggccgtgcc ccacgcttgg gccggtgccc acgattgggc cggtgcccac gcttcggccg
     8701 gggtcatagc agttgcattc gcaagggcca agatggcccg cgcggaccat gaccggcccc
     8761 tcgccctggt gggatcgcgg cgtccatgcc gaccggcggc cgttgttgct ggcgcgcggg
     8821 cgtgtccgtg atgctctccg ccactatttc gccggtgagg gcttcatcga ggtcgaatgc
     8881 ggcgcgctcc agacatcgcc gggcaacgag gcccatctgc acgcattttc aacggaacgg
     8941 atcggcaccg atggcgcgct caggacgctg taccttcaca cctcaccaga atttgcctgc
     9001 aaaaagctgc ttgcggcagg cgaacgccgc atcttcaccc tggcgcccgt ctatcgtaat
     9061 cgcgagcgcg gcgatctgaa cgcgtccgaa ttcaccatgc tggaatggta tcgcgccgag
     9121 gagccctatg cctgcgtgat cgtggactgc ctggcgatcc tgaagcaggc cgcgggcgcg
     9181 gtggaaaaca ctctcttccg cttccggggg atcgaatgcg atgtctccct caagcctgaa
     9241 cgcatcagcg tgtgcgatgc gttcaggctt tacgcaggca tcgatctctt ggcgacgctg
     9301 gacacggaag gcgcagcgga tcggaacgcg ctggcggggg cggcagaaac tgccggagta
     9361 aaaatcgcgg cagacgattc ctggtccgac atcttcagcc ggatactgac agagcgaatc
     9421 gaaccgcgtc tcggcctcgg ggcaccgacc gtgttggacc gttatccgcg cgtcgaagcg
     9481 gcactcgcgc gagcggcgtc cgacgacccg cgcgtggccg agcgcttcga gctttatgtc
     9541 tgcggcgtcg aactggcgaa tggcttcggc gaactgaccg acacggtgga acagcgccgc
     9601 cgcttcgaag cggagatggc cgagaaagaa cgcatctatg gagagcctta tccgatcgac
     9661 gaggatttcc tggcggcgct cggccatatg cccgaggctt gcggcgtcgc gctcggcttc
     9721 gaccgcctgg tgatgctggc gaccggcgcg cagcggattg gctcggtcat gtggaccccg
     9781 ccggtggcgt gacgcgcctc gtgcttcgtg attaatcgcc ggactcggat gcgcctgcca
     9841 agggaaagaa atatgctcga tagacgacgc acgttgcgga cgccggagga attggcggca
     9901 gccggactga tcgaagcctg tcaaaccggc gcgcttgccc ctgttgccga gcgctatgcc
     9961 atcgccatca cgccggccat tgcggacctc atcgacacgg ccgatcccaa cgatcccatc
    10021 gcgcggcagt ttgtgccctc gcccgccgaa ggcgacacgc acccgcaaga acgcgccgac
    10081 cccatcggcg atgagcgcca cagtccggtc gcgggcatcg tgcatcgcta tcccgaccgc
    10141 gtactgttga agctcaccca tgtctgcccg gtctattgcc gcttctgttt tcgccgcgag
    10201 atggtgggcc ccggggaaaa tcaggtactc ggcgcagacg aactggatgc cgccctcgcc
    10261 tatatcgccg cgcatgccga aatctgggaa gtgatcctga ccggcggcga tcccttcatg
    10321 ctgtcgccgc ggcggctggc gcaagtaacc gcgcggctgt cggcgattgc gcacgtcaaa
    10381 atcctgcgct ggcacacgcg catgctcagc gccgatcccg aacgcgtgac acccgaactc
    10441 gtgcgcgcga ttcgcacgga taagaccacc tatgtcgtcg cgcatgtgaa tcacccgcgc
    10501 gaactaaccg atgccgcccg gcgcgccgcg tcccgcctgg ttgaagccgg cattcccctc
    10561 ctgagccaaa gcgtgcttct caaaggcgtg aacgacgatt tagcgactct ggaggccctg
    10621 atgcgcgcgc tcgtggagtc gcgaatccag ccctattatc ttcatcacat ggatctggcg
    10681 cccggaaccg cgcatttccg aaccagtatc gccgacggtc aggcattggt gcgcgaattg
    10741 cgcgcccgga tatccggcct ctgccagccg cattacgtgc tggacattcc tggcggctac
    10801 gccaaggcgc tgctcaattc ttgcgatgcc gaacgggccg ggccactcca ccgcatccgc
    10861 gacggagagg gcaaattgca cgactatccc tttgcctgac gtgtcaaaaa agtgtgttcg
    10921 tgctatgagt gtgccacgag gggttgaaat gatcggcgca gcgccgatct atcgcaggca
    10981 ttcatcgtgc gcttgccggc tcgggaagcc caccttgtgc atatggagat aacgccatga
    11041 acaaactgat tctgccgttg ttgctcgcca cagccgcgtt cgccacaccg gcatcggcga
    11101 attggtttca caacccgtat caaggcatta accgcaatat cggctcggca ccgaacccca
    11161 cgcccgccga catccaggag atgcggttgc cccaggccac caaggtcgaa accccgatgc
    11221 tgaactatac ggtcgtggaa ccggctaagg acccgaacaa gaccgcgagt gcggccacct
    11281 cggccaatgc cccggcgcaa ggcgcaggca cggtaccggc ggcggcgccg tcgcgctgac
    11341 gccctgtttg caagatcaca gattggcggg cccgttggcc cgcctttttg ttgccgcaaa
    11401 cggcacaagt aacttattga tttctcaagc gaatgttgaa gctcttgacg gcggccccca
    11461 atgccgtttc gcgcgtttta tatgggaggc ttgctcaccc caaggagttc ccaatgaaaa
    11521 cggctaaaac cctgatgtca ttgctgcttc tatcgacggt gtcggtcgcg cccgcttacg
    11581 cgaactggtt cagcaatcca cggaccaata ccatgatgaa tatcggttca gcgcccaatc
    11641 cgacacccgc cgacctgcgg agcattggcg attccacagc ggtcgccctg aacggcaacg
    11701 cgggatcgcg agagccggtg gaacgtgtcg cctatgtggc cgcggaccgt tctcatgccg
    11761 tcaggcggga acaactgacg cgtctggagg gcaaggccgt atttggcgct catggcgtca
    11821 ggctcggttc gatcctgacc gttaacttgg ccgcacggat ggcagaagtt cagactccaa
    11881 gcggtatcgc ggtagccatg cccgtgggcc tgcttaggga tcggggaaac catgtcgcgg
    11941 caccgacgat ttcccgcgcg gatatgacgg ccatggccaa gacgcaaacc ggccatacca
    12001 ttgcgatcaa tatcgatcgg cgccgcccat cccgaggata gccatcgacg atagatcggc
    12061 tgtccgtcct gtaacgcgaa atcgagggcc atggcggaca accgccgtgg cccttgtttt
    12121 tgttggcaaa agtaacctta gagcggcaaa atttttcacc aaggacgcgt ctatgggtgt
    12181 aaatagggcc ggggggaggt tgcctcgtat ccgtgccgaa gagcacgaag gccgcctcaa
    12241 tgtatgcatg tgaggacaca tccactaaat ttcccagatc acgtgcagtc gcaatcgcat
    12301 agaaaatatg ccgctaacag gctggagcaa cggtcaggac atggatggta atgcggcgag
    12361 tggacagacc ggtgaatcgg ctgcctcgcg atcaagccaa agcgtagagc acattccggg
    12421 agtacgcgtg gacgtggata ccggccaagt ttcggatcga atatccggac ttcctccaac
    12481 caatatcaac gaccccagct atttccacaa agtcgtcgat tgccaatggg cgtgtccggc
    12541 acatacgccg gttccggaat atatccggct gatcgccgcg gggcgttaca gcgacgcgta
    12601 catggtcaac tggcattcga atgtgtttcc cggtgtgctc ggacgcacgt gcgaccgtcc
    12661 ttgtgaaccc gcctgccggc gcggccgcgt ggaagaaggc aaggcggcgc agcccgtcgc
    12721 catctgccgg ctgaaacgtg tcgccgccga ccacaaggac gatatccgta gccggctgcc
    12781 tgtcgcagcc attgcgaaga acggcaagcg catcgcgctg atcggggcgg gaccggcatc
    12841 gctgacggtc gcgcgcgatc tgcttccgct cggttatcat tgcgtggtct atgatgcgga
    12901 cgcatcggcc ggcggtatga tgcgcacgca gattccgaaa ttccgccttc ccgaaaccgt
    12961 tctcgacgag gaagtgaatt acatcctcgg tctcgagccc gaattgcggc tcggcaagcg
    13021 catcgaatcc atgaaggccg tcctggaaga aggctatgac gcgatcttcg tgggttgcgg
    13081 cgcgccgcgc gggcgcgagc ttgaagttcc gggacgcaag gaagcggctg cgaacattca
    13141 tatcggcatc gattggttgt ccagcgtttc cttcggccac atcgacaaaa tcggcaaacg
    13201 cgtgatcgtg ctcggcggcg ggaacacggc gatggattgc tgccgcacgt cgcgccgtct
    13261 cggcggcgag gacgtgaagg tcgtcgtgcg ttcgggtttc gaggaaatga aggcgtctcc
    13321 ctgggaaaaa gaagacgcga tgaacgaagg catcccgatc tcgaattatc tggtgccgaa
    13381 ggaatttacc catgatgacg gccgtctcac gggtgtcatt ttcgagaagg tcaaggcgga
    13441 atacgacccc aagggccgcc gcacgctggt tccttccggc gagcccgacg tgcacatgga
    13501 gtgcgacgat gtgctggtcg cggttggcca ggagaacgcg ttcccttgga tcgagcgcga
    13561 tctcggcatc gaattcgacc gctgggatat gcccaaagtg aataccgtca cgatgcaatc
    13621 gacgcatccg aaaatcttct ttggtgggga tgcggctttc gggccgaaga acatcatctg
    13681 ggcggtggcg cacggccatg aggcggccgt ttccatcgat ctcctctgca gcggcaacga
    13741 ggtgcatcac cgcccttccc cgctgacaaa cctgtcgagc cagaagatgg gcatccatga
    13801 atggagctat gacaacgata tctccgccga caagcgctac aaggttcccc atgcgccgcc
    13861 cgcattgacg ctgaaggacg tgcgcatgga ggtcgagctt ggtttcgacg tcaaacaggc
    13921 gttcatggaa gccgaacgct gcctcaattg cgacgtgcag acggtgttcg aagggcccaa
    13981 atgcatcgag tgcgatgcct gcgtcgacat ctgcccgatg gattgcatca ccttcaccga
    14041 aaacggtccg gaagtggagt tgcgccagcg tctgatcgcg ccctcctctc atcccgatac
    14101 gcaggatctc tacatctcgg aaaccttgaa gaccggccgc atcatggcaa aggacgaaga
    14161 cctctgcctg cattgcggcc tgtgtgcgga gcgttgcccg actggggcct gggacatgca
    14221 gaaattcctg ttcaacacgg ccaaggccgg gtgccatgtc taagccgatc aaatccatca
    14281 acgattttgt cgtcaaattc gccaatgtga acggttcggg ctcagcgtcc gccaatgaat
    14341 tgttcgcgcg cgccattctg cgcatgggtg tgccgatcgc cacgcgcaac atcttccctt
    14401 ccaacattca gggcctgccc acctggttcg aggtccgggt ctcggccaag ggatggggcg
    14461 gccggcgcgg cggttgcgat ctgatggtgg cgatgaaccc ccagacctgg gacaaggacc
    14521 tcgcctccat cgacgccggc ggctatctct tctacgattc ctccaagccg ttgccggaat
    14581 cgaaattccg cagcgacgtg aacatcgtcg gcatgcctct gacggagatt tgcaaccgca
    14641 cctatcacga tccgcggcag cgtcagcttt tcaagaacat catttatatc ggcgcgctgg
    14701 cagccattct ggagatcgac ctcgccgtgg tcgaaacgct gatcacggag caatacggcg
    14761 ccaagaagcc gcttttggag tccaacatcc aggccgtgcg catgggccac gcctatgcca
    14821 gggaaaacct ttccggcatt tgcggcttgc acgtggcgcg cgcgaacgcg gtgggtaacc
    14881 gtatcttcgt caacggcaat gatgcggcgg cgcttgggtg cgtctacggt ggagcgacgg
    14941 tggccgcctg gtatcccata acgccctcaa cctcgcttgc cgaatccttc atgaaatatt
    15001 gccgcaaatt gcgcgtcgat cccgagacca agaaaaacaa attcgccatc atccagtccg
    15061 aagacgagat ctcggcaatc ggctcggtcg tcggtgccgg ctggaacggc gcacgcgcct
    15121 tcaccgcaac ggccggtccg ggcatttcgc tgatgcagga attcctcggc ctcgcctatt
    15181 tcgcggaagt tccggcggtg attttcgacg tgcagcgcgg tgggccttcg accggcatgc
    15241 cgacgcgcac gcagcaatcc gaccttctgg tgtgcgccta tgccagtcac ggcgacacca
    15301 agcatgttct gctgttcccg caagaccccg gcgaattgtt cgaaatggcg gcccaggcct
    15361 tcgatctcgc ggaacggctg cagacgccga tcttcgtgat ggaagacctc gacatcggca
    15421 tgaacgattg gctgtgcgag cccttcgcct gggacgatgc gcgcaaatat gaccgcggca
    15481 agatcatgac cgccgaagat ctggatgcgg gcaagacttt cggccgctat ctcgatgtcg
    15541 atggcgatgc catccccttc cgcacgctcc ccggaaccca cgccaccaag ggcgcctatt
    15601 tcacccgtgg cacgtcgcgc gaccgcttcg ccaaatacag cgaagagggc tcggtctatg
    15661 tcgacaatat gcagcggctg gtgcgcaaat tcgaaaccgc gaaatcgctg gttcccgcac
    15721 cgattctaca caagtcccgt gaaacggccc gccacggcgt gatctatttc ggctcgacgt
    15781 cggctgccat ggccgaagcg ctggtggcgc tcgaatccga aggactgcac ctggatattt
    15841 tgcgcatccg ggcctttccg ttccccgaag cggtggtcga tttcattaac gcctacgacc
    15901 aggtctttct gatcgagcag aaccgcgatg cgcagatgcg catgctgctg gtgaacgaat
    15961 gcgccatcga tccggcccgg atcatctcgg tgctgcatta tgacggaacc cccatcaccg
    16021 cgcggttcat cacggcagca atcgcggagc gcttgcagat gaaagccaaa gaggcagcgg
    16081 aatgacctac atcgccaaac cgaaattcca tcatcccgca ttgccaaaaa attcgctcgg
    16141 cttcacgcat cgcgactatg aaggttcgat ttccacgctc tgtgccgggt gtggacatga
    16201 ttccatcagc gcggccgtca tccaggcctg tttcgagctg agcatcgagc cgcatcgcct
    16261 cgccaagctg tcgggcatcg gctgttcgtc gaaaacgccg gattatttcc tcggccaaag
    16321 ccacgggttc aataccacgc atggaagaat gccgtcggtt ttgaccggcg cgaacctcgc
    16381 caataaggat ttgctctatc tcggcgtctc cggtgacggc gattccgcct ccatcggcct
    16441 tggccagttc gtgcattgcg tgcgccgcgg cgtgaacatg ctctacatca ccgagaacaa
    16501 tggcgtgtac ggcctgacca aggggcaatt ctccgccacc gccgacaagg gctcgaagag
    16561 caaggcgggc ggcgccaaca atgattcccc gctcgatctg gtgggcatgg cgcttcttac
    16621 cggcgctaca ttcgtcgcgc ggagtttctc gggtgacaag aagcagctcg tgccgctgat
    16681 catggcggga ctgagccatc cagggccggc cttcatcgac gtcatcagcc cttgcgtcgc
    16741 gttcaacaat catccgggct ccaccaagag cttcgatttc gtgcgcgctc acaacgaggc
    16801 ggtgaaccgc ctggactaca tggacccgcg cgagcccatc attgtcgatt acgcgcccgg
    16861 caccgtcagc gaagtgacgc tgcacgacgg ctcgacgctg cgccttgcca agctgcaccc
    16921 cgaatatgat ccgcacgacc gcgtcggcgc gatgacctat atgcagagcc gtgcggcggc
    16981 cggcgaattg gtgaccggcc tgatctacat ggacccgcag cccagggaca tgcacaccat
    17041 gctcaacacg gtggacgcgc ccctcaatcg cttgagcgag aaagaattgt gtccgggttc
    17101 cgcggcgctg gaaaagatca acgcgggcct gcgctgacgc tgtggcgcgg atcatatttc
    17161 tgcttccatt cgcgaagcat gagatcaccg gcggtatcaa gacgacgtat cggcacgccg
    17221 aattgctcgc cgaattggga ttcgacgcct tcgtctatca acccggcggg ccgccggcct
    17281 ggttcgagac ccgcgccaag ctcctgacgc agcttgtgcc tgaccccggc gacattctgg
    17341 ttttccctga atcgctgaac ggaccgctga tggacatgat gcgccttgag gcgcgcaagg
    17401 tcctgttctg ccaagcgcac tactatacgc tgttcaacgc cgttgcgccc gagacctatc
    17461 gggatttggg gtttgagcgc gcggcgtgcc agagcaccat cgccaagggc tttctcgagc
    17521 gtgttctggg actttccaat attgccgttc ttccctgctt catcgacgga gagcttttcc
    17581 ttccccgcgc caaaagcatg cagatcgcgg ccatacccaa gaaattgccg cgggaagcgg
    17641 cggctattca gaagattttc catctgaaat atcccggcct ggccgcaacc cgatgggaga
    17701 tcatcgaggg ccgctccgaa cgcgaaacag cggagatttt cggccgctcc gccatcgttc
    17761 tatcgctgcc gtttctcgaa tccttcggct tggtgcctct ggaggcgatg gcgtcggggg
    17821 ccatcgttgt cggattcacc ggctacggcg cacaggaata cgcgaattcc caaaacggct
    17881 tctggtttcc ccccgaccat ctcgaagaag tggccgatgc gctcgcgcag gtgatcacgg
    17941 gattgcaaac aaacgatccg cagatctcga agatacgtga aaccggcttc gccaccgccg
    18001 cagtttacag caaagatcag acccgcgcgg cgctgaaatc attctatggt tccttgatgc
    18061 aggaccgggc gggagccagc gcatgaaacg cgattccagc actccggcgg gcacagtcac
    18121 ccggcaaatc gtcgacagcg aagtcctcaa aaccaatccg ctcggcgatt ccacgagccg
    18181 catcgtcgat gtgtatgtcc cggccggcca tgacggccac ggtttgccgc tgcttgtcga
    18241 tctcgtcggc ttcaccgcag gcggaccggc gcacaccaat tggcgcaatt tcaccgagaa
    18301 cattccagaa caggcggacc ggctgatcgc aaccggagcc atgccgcctt gcgtcatcgc
    18361 atttcccgat tgcttcacca ggctcggcgg caaccagtac atcaattccg cgggcatcgg
    18421 ccgctgggcg gatttcctgc tccaggaagc cgtgcccttt gtcgaacgtg cattcggctg
    18481 tggcggagcg ggcaaacgcg gcctcttcgg caaaagttcg ggcggttacg gcgccatcgt
    18541 ccatgctctg ctgtacccgg atttctgggc ggcatcggcc tgtcattccg gcgacatggg
    18601 tttcgaactc ctcaacattc ccgaaatgcc gcgcgtcttg cgcgcgttgg ccaaacaggg
    18661 cggcaccaaa ggctggctcg aagctttctt cgccaagcgc aaggtgaagg atgacgatat
    18721 ccatgtcctg atgatgctcg ccatgtgcgc gacctacgac ccggatccga gagcgttcat
    18781 gggcgtgcgc ctgccggtcg acatcgaaac ctgcaccttc attcccgagc gctgggccaa
    18841 ttggatgaaa tgggatccgt taaacctggt cgagatccat catgcgggat tgaagcggct
    18901 gaaggccctc ttcatcgatt gcggcgatat cgaccagtac aacctcgtct acggcgcccg
    18961 ccgcatgcac cgggcgctgc aaacctatgg agtcgcacat atttacgagg aatttccgga
    19021 cgatcattcc gggatcgact accgcatgga tcgcagcctg ccggtgctgg ccaaggcact
    19081 cgcgcgctag cgcgtgtcca attccgcagc atgagccgcc gcggaccgtg aaaatgcatc
    19141 agcgccggtt gcccggtaaa aaaggttcag taaccaattt cccctagatt aagccgcatg
    19201 gggagaggtt tcttaccgct cgtcgccgtg ctgctgtttg ctgcgtgcaa tccggcgggt
    19261 gcgacgaatt tcacggctga agtcgtcgat caggacggcc atccggtcgt gaacgccgtg
    19321 gtcagcctga tttccgaaac caaggcatcc atgcccgcgg cgttaacgca tctcgccaca
    19381 gaaaagacca tcgatcaacg caacgagacg ttcctgccgc tggtgaccat cgtgcccaag
    19441 ggcgggcatg tcgtctttgc caataacgat cagaccacgc accaggtcta ctccttctcc
    19501 gcgatcaaac aattcgagat cacgcttgcg cgcggcgaga aatcgcctcc cgtcgccttc
    19561 gagaaggccg gcattgccgc cttgggctgc aacatccacg atcacatgat cgcctacgcc
    19621 ttcgtggcgg aaagcccgtg gactgcgctg accggtgcag acgggcgcgt cacgatcgcc
    19681 gatgtgcctg caggaaatta tcaggcgcag gtctggcatc cgagatttcc gcccggccgt
    19741 gagccgccga gtctgcgtgc cgtgctgtcg ggcgatacca cgcacctttc cgtgagcgtc
    19801 aagctgccct atgccgccac cgcgtcccgc ggccatgcgg gcagctacta gggccatgcg
    19861 atgagtttcc gcaaacgcct tgcgctcttt ctgatggtaa ccctgatcgg ggtacaggtg
    19921 atcaccgcga tcttcgctta cggcgtcatc cgcggaaatt tggtcgaaca ggccaagaac
    19981 gaactcaaat cgaccgcgtc ggtcttcacc cgtcagctca acgtgctttc cgaacgcgtc
    20041 agcgacgatg tcgaagtgct ctccctcgac tacgcgcttc gaaaggccgt cgcggagcac
    20101 gatcaggcca ctgtgctttc cgcgctgcgc aaccacggca atcgtgtcgg tgccacgcgg
    20161 atgatgctgg tgggactcga cggaaagatc acggccgata ccacggacga acgcgcggtc
    20221 ggaaccgcgt ttcagttttc cgacctgatc gaaactgcga gcgcgaacga tcaaggcaca
    20281 tcgctcgccg ttctggacgg cgcgatctat tggattgtgg tcgtgccggt ccgcgcgccc
    20341 gttcccatcg cgttcattgc ggcatgcgtc ccggtgaacg atgcgcttct ggaaaagctg
    20401 cgccagcttt cctccgttcc acaatcgctc gcgcttgcta ccaccgatgc gcgcgggaat
    20461 tgggctgtcg tttcaaaaac cgcaggttac gcgccgacag tgcggctcac atcccagaca
    20521 gcgtcgatac cgcaggattc gattatcgca accgagcaga aggacgatcg cctcgcgatg
    20581 atggcgcggc tggcgacctc ccaatccagc cgtcccgtcg ttgccgtcct cgactattct
    20641 ctggaagaag ccctgaacgc ctatcgcgtg gtgatcacac ccatgcttct ggtgctgggc
    20701 ggcgcgctgc tggttgcgct cgtgggggcc atgctgatcg cgcatggcgt ctcgcgaccg
    20761 ttggagacgc tcgcggccac cgcacggcgc attgccaagg gcgactacac accgatgccc
    20821 gcaatcggcc gcagcgatga gatcggcgag cttttgctgg cgctgaacaa catgacgcaa
    20881 tcgatcgccg aacgcgaaac cgcgctcaag agtgcggtcg cgtctctgga actggcgcgc
    20941 aaccaggccg tgaaggccag tgaagcgaaa tcgcaattcc tttccaacat gagtcacgag
    21001 cttcgcaccc ccttgaatgc gatcatcgga ttttcggaga tgattcatcg tcaggtgctc
    21061 ggtcccatcc cagtccagcg ttatgtcgaa tatgcgcacc acgtcttcga tagcggcagg
    21121 cgcctgcttt tccaggttga agaaatgctg gacctgtccg acgcggaatc cgacaaacta
    21181 tcgctggcgc gcatatcttt aaagccaggc agcctgttgt cggccagcct cgaaactctc
    21241 gcaccattcg ccgcgaaatc cggcgtgagg ctggatatct gcggtgagat ggggtcatgg
    21301 ccgctcatcg ccgcggacgc agcgaagctg gagcagagcc tgaccaatgt cgtgcacaac
    21361 gccatcaagt tcacgccggc cggcggcctc gtaacggtgt ccggaggttg cgccggcagc
    21421 atgttgaaga tcaccgtttc tgatacgggt gtcggcatcc gttccgaaga tctcgacctc
    21481 gtggtccgtc cgttccatcg caggaagtcc gcattcgatg ccgtgtatca gggcgccggt
    21541 ctcggacttc ccttcgccaa gaccatcatc gatctgcatg gcggcacact cgccatccaa
    21601 agcacccagg gcatcggcac gacggtcacg atggagttgc cgctggccat ggatgccaca
    21661 gccctcaacc acgcggcgtg acagatatgc gcaaactgct cgcggcactt ttcctgatcg
    21721 gttcctgctc ggccgcgttc gccgtcgatt ttcatttcga cggctatgtc gatctgcggc
    21781 tgatcgtgcc gtcgaaccag gagtcgtggc aagacggcca gttgggaaag cttcgctatg
    21841 gagccgaaga cggcaaaccg gaactgcgca tggccgaagc ggtcggacag ggagtggcct
    21901 tgatcacgcc ggagctgatg gctttggcgg tggtgcgcat cgagcccgag caacgcacgt
    21961 tcctcgattt tctcgaggct tatgcgcgat accggccggt atcgacgacg ccgtggcgct
    22021 ggtcggtcaa agcgggagcc ttcttcccgc cgatctcgct cgagaacacc gaaatcggct
    22081 ggaccagccc gtggacgctg acgccttcgg caatcaatac ctgggtcggc gaagaactgc
    22141 gcaccatcgg tgtggaaggc gcgctcgaat ggcgcagcga ggaacgcacc atcacggcca
    22201 tggcctctgt cttcggctgg aacgatccgg ccggcgccct gatcgccgac cgcggctggg
    22261 cgctgcacga ccgcgtaacc ggactcatcg accgtccgcg cgaacccgat gtcatcgccg
    22321 caaaaatcgg ggtgagcgta ccgctctata cctatgaggc gctcgagatc gacgaccggc
    22381 ccggttggta tgcggccgca tcgtgggacg aagccggttt gggatttctc aacctgatct
    22441 attacaacaa tgaggccgat ccggcggcgg ttcgcaggga cacgatagcg tggcggaccg
    22501 acttctggga tgtgggcgca cgcacccaat tcggcgacgt cacgctgatg gcgcaggcta
    22561 cgaccggcca gaccatgatc gtgccgaatg ccttcttctt cagcgatacc acgttcaaat
    22621 ccgcctatct gctggcggga tggacgatcg tgcaggactg gcgtgtcgct gcgcgggccg
    22681 atgtgttctc caccgacgaa aaaaatccct ttcctggaaa ccagagcgag cacggcaacg
    22741 cactgacgtt tgccgtgaac tatctgccct atgactggct tcgcctgacc gccgaagcga
    22801 tccgcgtcga cagtacgcgc aaccagcgga cattggcggg gctgaacccg cgcgccgtgg
    22861 aaaatcagct ccagctcagc gcgaaattct atctgccctg atcgagcgga tagcccttaa
    22921 gtccgctcac gccatagatc gcatattcgt cgatcgtggc cccatttcgg acgcgtgaaa
    22981 tatgggcgag cggcataatc cgtgcaaaat gcgccgccaa tagctgagat tggtcgttgc
    23041 gcgtttcggt cacgtaaagc aacggacctt cgaacaaagc gcgcggcggc tccggctcat
    23101 cgagatagcg aacgcgctca ttcacctgca ccacgggcgg gtgcgacggc agatagaagc
    23161 tgagccatcc cgtcagtgcg taacccgttg tgagaaccgc acgcgcatgc gtccgattgc
    23221 gcagcgcttc gatctggttc gcaaccgggt caattccgaa cgcgagcagc cgcgaaaccg
    23281 gatcgcgaac ccgcgggatg atcccccaga gcgcctgggc atagacgacc gccagcattg
    23341 cccccgcgaa cgggacggca atgcgccgcg aaagccgcag gaaccagggc gatcccttct
    23401 gctccacgcg caagcaggtg gccgcggcgg caagcacaaa ggcagggtac aggaaggacg
    23461 gccagtttcc ctgcacgcga tcatgcagcg cgtgccaggc gaaatagatt accggcggcg
    23521 cgatcatcgc gaaaagaagc gcgggctcgg accgcatccc ctcgcgtgac cgaaagatca
    23581 tgaccacgcc cgccacgccc aatatggcga tgaacggggt ggcgagcgcc aattggcccc
    23641 cgagaaattc cacaagaaat cgcaaagtga acccgccctc atccagacgg ccgaattgtt
    23701 ttgcgaagga aatccaatcg tgctccgcat tccacaaaag aacgggcacg aacatcagca
    23761 gtccaatggc ggcgcctaga tagggccaga acgatagaaa ccatcgccgc tcccgaggca
    23821 cgatggcaag ccaggccaga attcccacgc cgagaaagaa gccagtgtat ttggacaata
    23881 gcgcaaagcc cgccgcgaca ccggccgcga tccaccagag gccatttccc gtttccgcga
    23941 ccttcgccag cgtaagcaaa agaaacgcgg ccgccgcgat ttgcggtgag tccggcgtgg
    24001 cgaccagcgc ctcaacgccg atcatcggca tcacattgaa gaacaatgcc gcgagcaccg
    24061 cgccatattc gctcttgaga ataaggcgtc cagaccgcca caccgcccaa cttgccagaa
    24121 cggacagcag ccacggcacg aagcgaacgc cccagctcgt gtctccgaac aaggctgttc
    24181 cggcggcgat cgtaaaggcg atcaatggcg gatgatcgag atagcctgcc gcaagatgct
    24241 ttgaccacag ccagtaatag gcttcatcat acgaaagcgg cgtcaggccc gcgacaatgc
    24301 cgcgaaccag gatgagagcg agaacacaga gcaccaccgc gcgaatagga ttctcatgag
    24361 agatcgatct catgcaatac gccgccgtgg gaggaaaaag acgaagggga tgtgcggcat
    24421 ccgatgagcg actcgggcgg gagcggtcag accaaggcgg tggtagcgga tggttcgtgc
    24481 tcccgcaagg acagtcccat cgcctcttgg gccggcttgc gggaatccat taaaaaaatc
    24541 gccagagcgt ccagccgttt acgcatgaaa cctgcgcttg gataatgctc cgcgtctcct
    24601 tatcatcgcg cccgcccggc caacaggcga agcgcggagg acagggtgca agagaacgag
    24661 atcagcttca aaccgaaaaa atccgtcgct ctgtccggag ttgtcgccgg gaatacggcc
    24721 ctgtcgacgg tcggccattc cggcaacgat ctgcattacc ggggctacga cattctggac
    24781 atcgccaatc gctgcgagtt cgaggagatc gcccatctgc tggtgcatga gcggctgccg
    24841 ggcgccggcg aactcgcggc ctacaaggcc aaactccgcg cgatgcgcgg cattccggcc
    24901 aatgtgagga cggtgctgga agcgatgccg gccgcggccc acccgatgga tgtgatgcgg
    24961 accgccgtgt ccgcgcttgg ctgcatcgcg cccgaaaaag acggccacaa tcc
//
LOCUS       DASYEG010000070         2308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4948079, whole genome shotgun sequence.
ACCESSION   DASYEG010000070 DASYEG010000000
VERSION     DASYEG010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4948079"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VGM72_02735"
     CDS             <1..319
                     /locus_tag="VGM72_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264207.1"
                     /translation="DFNDASNFLDLFISGGGNNWGGYSNKEFDRMLNAAKADPNSETR
                     GAKLAAAEAILLKDQVVMPLFFWVSGNLVRPYVKGWQANALDKHRARWISIDEKARAA
                     TLG"
     gene            373..449
                     /locus_tag="VGM72_02740"
     tRNA            373..449
                     /locus_tag="VGM72_02740"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:407..409,aa:Arg,seq:cct)
     gene            complement(451..>2308)
                     /locus_tag="VGM72_02745"
     CDS             complement(451..>2308)
                     /locus_tag="VGM72_02745"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEY4264208.1"
                     /translation="AYEKLSGKVEFALDPKLAINKNIIDLDLAPKNAQGQVEFTADFM
                     MLKPVDPSRGNGRLFDEVGNRGGKAMLSMFQKAKGSRDPQTAEEFGNGALMNQGYTLV
                     WMGWQWDVPNGQMRMDMPIATNGGKKITGLVRGNFIPNDNSPTQSLADRNHLAYPIDD
                     PDGPDSVMTVRDRAADKPQVIPRAKWHFVGDASVSLDGGFELGRIYDVVYRARDPRVV
                     GTGLAGTRDLVSFLKYDRTPANPMPTIKYAYGWGVSQSGRFLRQFLYEGFNEDEQGRQ
                     VYDGVLDEVGGAGRGSFNYRFGQASRDAEQFFNVFYPADMFPFTDAVESDPILGKTDS
                     MLGPAEARHVAPKIFHIFSNSEYFNRAGSLIHTDVTGTRDVAPPANARIYFVSSGPHA
                     FGPMPPVQATGAAALNNPVQRNPVVRALLRDMDQWVVEGTLPPDSRIPHIADGTLVPT
                     EKAGWPNIPGVHFPVPYLKTYRLDFGPDWSKGIVREPPVVGQIYVNLVPAVDANGNSR
                     AGIRLPAIEVPVGTYGGWNFRDPAIGSGDQLFGEMGSFHPFARTKAQREASGDSRLSV
                     EERYASREAYRAKADAVAKKLVAERLVLPEDEQEPVDLAVSLYDWASSPDRK"
BASE COUNT          459 a          726 c          684 g          439 t
ORIGIN      
        1 cgatttcaac gacgcgtcga atttccttga cctgttcatc agcggcggcg gcaacaattg
       61 gggcggctac agcaacaagg aattcgaccg gatgctgaac gccgccaagg ccgatccgaa
      121 cagcgagact cgcggcgcca agctcgccgc agcggaagcg atcttattga aggaccaggt
      181 ggtgatgccg ctcttcttct gggtcagcgg caatctggtc cggccttacg tcaaaggctg
      241 gcaagccaat gcgctggaca agcaccgcgc ccgctggatc agcatcgacg aaaaggcccg
      301 tgccgccacg cttggatgac aggtttcgcc actggccttg ccgcagaaaa tcgggcaatt
      361 tcccgcacaa gcgaccccat agctcagcag gatagagcgg cggtttccta aaccgtaggc
      421 cggaagttcg agtcttcctg gggtcgccag ttatttccga tccggcgacg aagcccaatc
      481 atacagcgac accgccagat cgaccggctc ctgctcatcc tcgggcaaca ccagccgttc
      541 ggccaccagt tttttggcca ccgcatcggc cttggcgcga tacgcctcgc ggctggcata
      601 gcgttcctcg accgagagac gggaatcgcc gctggcttcg cgctgcgcct tggtgcgggc
      661 aaaggggtgg aaggatccca tctccccgaa aagctgatcg ccggacccga ttgcgggatc
      721 gcggaaattc cagccgccat aggtgccgac cggaacttca atggcaggaa gccggatacc
      781 tgcgcggctg ttgccattcg catccaccgc cggaaccaga ttcacgtaaa tctgcccgac
      841 caccggcggc tcgcgcacga tgcccttgct ccaatccgga ccgaaatcga ggcgataggt
      901 tttcagatag ggaacgggga aatgcacacc cggaatgttc ggccagcccg ccttctcggt
      961 cggcaccaac gtgccatcgg cgatatgcgg aatgcggctg tccggcggca gcgttccctc
     1021 caccacccat tgatccatgt cgcgcagcaa tgcgcgcacc acgggattgc gctgtacggg
     1081 attgttcaga gcggccgcgc cagtcgcttg aaccggcggc atgggaccga aagcgtgcgg
     1141 gcccgacgac acgaaataga tccgcgcatt ggcgggcggc gcgacatccc gtgtgccggt
     1201 cacatcggta tggatgagcg aaccggcgcg gttgaaatat tccgaattgc tgaagatgtg
     1261 aaagatcttt ggcgcgacat gccgggcctc ggcggggccc agcatcgagt ccgtcttacc
     1321 caggatggga tcgctctcca ccgcgtccgt gaaaggaaac atgtcggcgg gatagaagac
     1381 gttgaagaac tgctccgcgt cgcgcgatgc ctggccgaaa cggtagttga aggatccgcg
     1441 tcctgcaccg cccacttcgt ccagcacgcc gtcatagacc tggcggccct gttcgtcttc
     1501 gttgaagcct tcataaagga actgccgcag gaagcgcccg ctctgcgata cgccccagcc
     1561 ataggcatat ttgatggtcg gcataggatt ggcgggcgta cggtcatact tcagaaagct
     1621 gaccagatcg cgcgtgcctg cgaggcctgt gccgaccacg cggggatcgc gcgcacgata
     1681 gaccacgtca tagatgcgcc ccagctcgaa cccgccatcc agtgaaacgc tggcgtcacc
     1741 cacgaaatgc catttcgcgc gcgggatcac ttgcggcttg tccgccgcac ggtcccgcac
     1801 cgtcatcaca ctgtccgggc cgtcgggatc gtcgattgga taggcaagat ggttgcgatc
     1861 ggccaaagac tgggtcggcg aattgtcgtt gggaatgaaa ttgccgcgca ccaggccggt
     1921 gatcttcttg ccgccatttg tggcgatggg catatccatc cgcatctggc cgttcggcac
     1981 atcccattgc cagcccatcc agaccaaggt gtaaccttgg ttcatcagcg cgccattgcc
     2041 gaactcttcc gccgtctgcg gatcgcggct gcctttcgcc ttctggaaca tcgatagcat
     2101 cgccttgccg ccgcgattgc ccacttcgtc aaacagcctg ccattgccgc gtgacggatc
     2161 gacgggcttc agcatcatga aatcggcggt gaattccacc tgcccttgcg cattcttcgg
     2221 cgccagatcg agatcaataa tgttcttgtt gatggcaagt ttggggtcga gcgcgaactc
     2281 cactttgccg ctgagctttt cataggcg
//
LOCUS       DASYEG010000071         4701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_601717, whole genome shotgun sequence.
ACCESSION   DASYEG010000071 DASYEG010000000
VERSION     DASYEG010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601717"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="VGM72_02750"
     CDS             complement(<1..1014)
                     /locus_tag="VGM72_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264209.1"
                     /translation="MLNYNPYFSIGDAVAGLGIFLLIPQFLKPIYVFRLRVLGIGLRT
                     LYAVSGLGFLCVVTAALCAHYNDSFPSFLGNPFGWEMAGGFLYAAAYSALGLVYIFPA
                     RASLGSITKYVRAGAQFLASASEEDRVEFVADVLANIRKLIRIADLAGSAPRRMTSLI
                     LRLRHRSTTDIAAYSQSFLGLLADPVFCRTLVSRLPWDAARILHAFPLEKPAGQVGRT
                     FVHQLTRYALIAAETMGAKEDEWMGFSDAPALSHAAFGNTYLNRYYLPWDGLTAADLK
                     GADLPMLERIARAAQLTIDEYAAGGFSYHSYNIAKLQESYEVLSRHLYQLKKTDADIS
                     QFSG"
     gene            complement(1091..4018)
                     /locus_tag="VGM72_02755"
     CDS             complement(1091..4018)
                     /locus_tag="VGM72_02755"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEY4264210.1"
                     /translation="MRRAAHHVPDAAFARLAEQLQADAWASRMIYDHEGREEAIRIML
                     RPLLVMPQQLSYVHHVCLQLVEALKRLPALYLGDPRIRRVLAITPDEERRLRDTWTPA
                     HQQFNPIYGRLDAVCDFTGAGWQDSLHFMEPNLSGVGGIHFAPVAEDLVLRDVVPTLL
                     AQNPELVVSLPRDQRDLFVQVLIDHARDIGRETCQLCFVEAKYVHDGPNEQSVLSSYL
                     ARKHDLTIAHADPRELRVVGDEVYCADIRIDVAYRDFEMRELIAREKETGERLDAMRL
                     LFRQNRMISSIVGDFDHKSGFEVLTDPVLAGEYFGADDLRLFRRHVLWTRLVGERKTT
                     LPGGREADLMAYARRNREILVLKPNRSYGGIGVVLGAATGQAEWEHLLDEAAALCGDP
                     ERSSVVQLATRLPVHEFPVVSSDGRVFGEPFYAVMGFAATDNGLGTMCQVSQKQVVNV
                     AQRGGLAAVLEAEAPPELTIPTRAVNRAAGTRELREQIRELRHLDHTIALLGWDEETM
                     LVPAGREERGEQLATLEGLRHALLTSGNLGDLVENIAAKPNADPACAREMQLLQRLRR
                     HALALPQDLVRHLANARSHALGAWEEARTRDDFGIFSQPFDQLLGLLRDKAAALAAGG
                     DPYDALLDEYEEGVTRARLDPVLRDVRERLVPLVRQASEATARYAPLLEGRNFAANEQ
                     WELCRKLLAAIGFTFGRGRLDRSTHPFTLYAGINDVRLTIRVDESDLSSAILATMHEG
                     GHGLYDQGFDPADRDTLLGEAPSMALHECQSRLWENHVGRSPAFWRYMFPILQASFPN
                     AVRGLDGEAICRAVNVVRPGVNRVAADEMSYHLHIVLRYELELALLSGSLPVRDLPQV
                     WRERSATLIGATPASDREGVLQDVHWSLGSFGYFPTYTLGSLYAAQLAESYARESPLE
                     EQIEHGDFAGLLGWLRSHVHRIGSRLQAEDIIRNATGNGLNSDAFFRHLERAIPRGSV
                     SAS"
     gene            4235..>4701
                     /locus_tag="VGM72_02760"
     CDS             4235..>4701
                     /locus_tag="VGM72_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4264211.1"
                     /translation="MSNIKSIGIGREKKSVKSRGEALRDYVLRRLLLMIPTFLGITFV
                     TFLLCQFVPGGPIDQLRLELAGAGGEFGGRSSRVQLEIPDDQLKQLNAYYGFDKPVVV
                     AYGLWLVKTVQLDFGQSFRYNEPVLRVIAERMPVSIYYGIVTAIFTFAICIPLG"
BASE COUNT          937 a         1425 c         1489 g          850 t
ORIGIN      
        1 gcctgaaaat tgcgagatgt ccgcatcggt cttcttcagt tgataaagat gccggctcaa
       61 cacctcgtag ctctcctgca atttcgcgat gttataggaa tgataggaaa acccgccggc
      121 ggcatattcg tcgatggtta gttgtgccgc gcgggcgatt cgttccagca tcggcaggtc
      181 ggcgcctttc aaatcggcgg ccgtcagtcc gtcccaggga agataataac gattgagata
      241 ggtgttgccg aaggccgcat gcgacagagc cggcgcatcg gagaacccca tccattcgtc
      301 ttctttcgca cccatggttt cggctgcgat caatgcgtag cgtgtgagtt gatgaacgaa
      361 ggtgcggccc acctggccgg cgggtttctc caacggaaat gcgtgcagga ttcgtgcggc
      421 gtcccaggga agacgcgaca ccagagtgcg gcaaaacacg ggatccgcga gcaatccgag
      481 aaaagactgg ctgtaggcgg cgatgtccgt ggtgctgcgg tgacgcaaac gaaggatcag
      541 cgatgtcatt ctgcgtggcg cggagcccgc caaatccgcg atgcggatca gcttgcggat
      601 gttcgcgagc acgtcggcga cgaattccac ccggtcttcc tccgaagcgc tggccagaaa
      661 ttgcgcgccc gcccggacat atttggtgat cgaaccaagg ctcgcgcggg cgggaaaaat
      721 gtagaccagg ccgagcgcgc tgtaggccgc cgcatagagg aacccgccgg ccatttccca
      781 gccgaatggg ttgccgagga aagatggaaa cgagtcgttg taatgggcac aaagcgccgc
      841 tgtcacgacg cagaggaacc ccaggccgga aaccgcatag agcgtgcgca gcccaatccc
      901 gagcacccgc aggcgaaaga catagatggg tttcaggaat tgcgggatca gcaggaatat
      961 gccgagaccc gcaacggcat caccaatcga gaaataggga ttgtagttca gcaatggccc
     1021 cgggcccctc gcgcctgtcg tctatctgta acccgaagcg accttccatc gccagacttt
     1081 gtgcggtgtt tcaggaagct gacaccgaac cgcgtgggat tgcgcgttcc aaatggcgga
     1141 agaaagcatc gctgttaaga ccgtttccgg tcgcgttccg gatgatgtct tccgcctgga
     1201 ggcgcgagcc gatccggtgc acatgggacc gcagccagcc cagcaggccc gcgaaatcgc
     1261 cgtgttcgat ttgctcctca agaggagatt cgcgcgcgta gctttccgcc aattgcgcgg
     1321 cataaagact tcccagcgta taggtcggaa aatagccgaa gctgccgagc gaccagtgca
     1381 cgtcctgcag cacgccttcg cggtccgatg cgggtgtcgc tccgatcaag gtggcgctgc
     1441 gctcgcgcca gacctggggc aaatcgcgga ccggaaggct tccggaaagg agcgccaatt
     1501 ccaattcata gcgcagcacg atgtgcagat gatacgacat ctcgtcggcg gcgacgcgat
     1561 tcactccggg cctgacgacg ttgacggcac ggcagattgc ctcgccgtcg aggcctctga
     1621 cggcattggg aaaggatgct tgcagaatcg ggaacatgta gcgccagaag gcggggctgc
     1681 gtccgacatg attctcccag aggcgtgact gacattcgtg cagcgccatg ctgggggctt
     1741 cgcccagcag tgtgtcgcgg tcggcgggat cgaagccctg atcgtacaga ccgtgtccgc
     1801 cttcatgcat ggtggcgagg atcgccgagg acagatcgct ctcatcgacg cggatggtga
     1861 ggcgtacatc gttgatgccg gcatacagcg taaacggatg ggtcgagcgg tcgaggcgtc
     1921 ctcgcccgaa cgtaaatccg atagccgcga gcagtttgcg gcacagctcc cactgctcat
     1981 ttgcggcaaa attccggccc tcaagaaggg gcgcgtagcg cgcggtcgcc tctgaggctt
     2041 gccggacaag cggcacgaga cgctcgcgca catcccgaag caccggatcg agccgtgcgc
     2101 gcgtcacgcc ttcttcgtat tcgtccagca gcgcatcgta gggatcgcca ccggcggcga
     2161 gcgcggcggc cttgtcgcgc aacaggccga gcaattggtc gaagggctgg gagaaaattc
     2221 cgaaatcatc gcgtgtccgt gcctcttccc aggctcccaa cgcatgggaa cgcgcattgg
     2281 cgagatgccg caccaggtcc tgcggcagag cgagagcgtg tcgccgcagc cgctgcagca
     2341 attgcatttc acgcgcacag gctggatcgg cgtttggttt cgcggcgatg ttttccacca
     2401 ggtcgccgag attgccggag gtcagaagcg catggcgcag gccctccagc gtcgccaatt
     2461 gctcgccacg ctcctcgcgg cccgcgggga cgagcatggt ttcctcgtcc cagccgagga
     2521 gcgcgattgt gtggtcgaga tggcgcagct cgcgaatctg ttcgcgaagt tcacgcgttc
     2581 ccgcggcgcg attcacggcg cgcgtgggaa tcgtgagttc cggcggtgcc tccgcttcca
     2641 gcaccgcggc aaggccgccg cgctgggcga cattcaccac ttgtttctgg gaaacctggc
     2701 acatggtgcc cagcccgtta tcggtggccg caaaacccat cacggcataa aaaggttcgc
     2761 cgaacacgcg tccgtcggag ctgacgaccg gaaattcgtg gaccggaaga cgcgtcgcca
     2821 gttgcacgac ggaggaacgt tcgggatcgc cgcaaagggc ggccgcttcg tccaacaaat
     2881 gctcccattc ggcttggccg gtggccgcgc ccagcacgac gccaataccg ccataggagc
     2941 ggttgggttt cagcactagg atttcgcgat tgcgccgtgc gtaggccatg aggtcggcct
     3001 cgcgcccgcc gggaagcgtt gttttgcgct cgccaacgag ccgggtccac agcacatgac
     3061 ggcggaacag acgcaaatcg tctgctccaa aatattcacc ggcgagcacg ggatcggtga
     3121 gcacttcgaa gccgctcttg tggtcgaaat ccccgacgat ggaggagatc atgcgattct
     3181 ggcggaacag aagacgcatt gcatcgagcc gttcgccggt ttccttctcg cgcgcgatca
     3241 gctcgcgcat ctcgaaatcg cgataggcga cgtcgatccg gatgtctgcg caatagacct
     3301 cgtcgcccac gacccgcagt tcccgcgggt cggcatgggc gatggtgaga tcgtgtttgc
     3361 gcgccagata gctgctgagg acggactgct cgtttggtcc gtcatgaacg tatttcgcct
     3421 cgacaaaaca gagctggcag gtctcgcggc cgatatcgcg ggcgtggtcg atcagcacct
     3481 ggacgaaaag atcgcgctgg tcgcgcggca ggctgaccac gagttcagga ttctgcgcaa
     3541 gcagcgtcgg caccacatcg cgcaggacaa gatcctcggc caccggcgcg aaatggatgc
     3601 cgccgacgcc cgagaggttc ggttccatga aatggagcga gtcctgccag ccggcgccgg
     3661 tgaaatcgca taccgcatcg agccggccat aaatcggatt gaattgctga tgcgccggcg
     3721 tccaggtgtc gcgcagccgg cgttcctcgt ccggggtgat cgcgagcacc cggcggatgc
     3781 gaggatcgcc cagatagagc gcgggcaggc gcttcagcgc ttcgacgagc tgaagacaaa
     3841 catggtggac gtaagagagc tgctgcggca tcaccagcag cgggcgcagc atgatgcgga
     3901 tggcttcctc gcgcccctca tggtcgtaga tcatccggct cgcccaggcg tcggcctgaa
     3961 gctgctccgc aagccgcgcg aatgccgcat ccgggacgtg gtgcgccgcg cgcctcaccc
     4021 gcccctccag agtggctcct gacagcccca agcttcggca ggactgagaa tggaccgcat
     4081 gcgccagggg cctcccgaac atttcatcaa tcgccagaga ccattccagg ctcataaaat
     4141 ccaggccctc atgcgccagc gccgcgggtg acggcggcgg aaaagccctt aatcagggag
     4201 cagggccaaa cttgacagtg acggcgcaag ctaagtgagt aatatcaagt ccattggcat
     4261 cggcagggag aagaaatcgg tgaaatcacg cggcgaggct ttgcgcgatt atgtgttgcg
     4321 gcggctcctg ctgatgatcc ccaccttcct cggcatcacc ttcgtgacgt tcctgctctg
     4381 ccaattcgtt ccgggcgggc cgatcgatca gctccgcctg gaactagccg gcgcgggtgg
     4441 cgaattcggc ggcagaagct cgcgcgtcca gctcgaaatt cccgacgacc agctcaaaca
     4501 gctcaacgcc tattatggct tcgacaagcc cgtcgtcgtt gcttacggtc tgtggctggt
     4561 caagacggtg cagttggatt tcggccagtc cttccgctat aacgagccgg tgctgcgcgt
     4621 cattgccgaa cgcatgccgg tctcgatcta ttacgggatc gtgaccgcca tcttcacctt
     4681 tgcgatctgc attccgcttg g
//
LOCUS       DASYEG010000072         1612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4661152, whole genome shotgun sequence.
ACCESSION   DASYEG010000072 DASYEG010000000
VERSION     DASYEG010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4661152"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VGM72_02765"
     CDS             complement(<1..377)
                     /locus_tag="VGM72_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264212.1"
                     /translation="MIVKPQEADRFAANPPKGLRVALVFGPDAGLVQERAEKLLKSVV
                     ADLTDAFNVADLSESVLLSDPARLSDEASAISMMGGRRVVRVRGAGNNLTDLFEEFLE
                     SDERPGDALVVVEGGDLAKGTGLR"
     gene            complement(374..850)
                     /gene="lptE"
                     /locus_tag="VGM72_02770"
     CDS             complement(374..850)
                     /gene="lptE"
                     /locus_tag="VGM72_02770"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEY4264213.1"
                     /translation="MRAAAVLGCLFLSACGFHPMYGGGKLAPQLAAIYVEPVAERDGY
                     ELRNQLIDLLGSDGREAGKVYHLKLTINQVSNGVTLQNDATITRYNDTVTATYVLTDA
                     HGTEMTRGSQTSLSSFNVSKSPYSSLVVQQDADRRAADDLADRIRMDLGAYFGGRR"
     gene            complement(937..>1612)
                     /locus_tag="VGM72_02775"
     CDS             complement(937..>1612)
                     /locus_tag="VGM72_02775"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY4264214.1"
                     /translation="EAIISDYGADTIRWFMLSDTPPERDIEWTDAGAEGCWRFVQRVW
                     RLASEAEGLPAPGTAAAPADDVSKALRQAAHKAIAAVTEDLSGLRFNRAVAQLYMLSN
                     AIADASKADAATRREALEALVLLSAPMMPHLAEECWQALGHTKLVAETSWLKHDPALL
                     KTDTVTIAVQVNGKRRGEVTVARDADNKTVEAAALAEDGVKRALDGAAPKKVIVVPGR
                     IVNIVA"
BASE COUNT          296 a          538 c          492 g          286 t
ORIGIN      
        1 cgcaggcccg tgcccttggc gagatcgccg ccctccacca ccaccagcgc gtcacccgga
       61 cgctcgtcgc tttccagaaa ttcttcgaac agatccgtca gattgttgcc ggcaccgcga
      121 acccgcacga cgcggcgccc acccatcatc gaaatcgcag aagcttcatc cgacagccgt
      181 gccggatcgg acagcagtac gctttcgctc aggtctgcga cattgaaggc atcggtgaga
      241 tcggcgacca cgcttttcaa aagcttttcc gcgcgttcct gcaccagacc ggcatcggga
      301 ccgaacacca gcgccacgcg cagccccttg ggcggattgg cggcgaagcg atccgcctcc
      361 tgcggcttga cgatcatcgc cgtccgccga aataggcgcc cagatccatg cggatacggt
      421 cggccagatc gtcggcggcg cggcggtcgg catcctgctg caccaccagc gaagaataag
      481 gcgatttgga gacgttgaag gacgagaggc tggtctgact gccgcgcgtc atctcggtgc
      541 catgcgcatc ggtcagcaca taggtcgcgg tcacggtgtc gttgtagcgg gtgatggtgg
      601 cgtcgttctg caaggtgacg ccgttcgaca cctgattgat ggtgagcttg aggtgataaa
      661 ccttgcccgc ctcacgcccg tcggaaccca gaaggtcaat cagctgattg cgcaactcat
      721 agccatcacg ctcggccacc ggctcgacat agatcgccgc caattgcggc gccaattttc
      781 cgccgccata catcggatgg aacccgcaag cagagagaaa caagcacccc aacactgccg
      841 cagcacgcac aagcccgcct cccccgcgcg cgaagcgctg gtgggggagg tgcccgtagc
      901 accgatcagg tgcgaaggcc ggagggggca aaaaactcat gccacgatgt taacaattcg
      961 gcccggcacg acaatgacct ttttcggcgc cgcgccatcc agggcccgtt tcacgccgtc
     1021 ttccgccaaa gccgcggctt ccacggtctt gttgtcggcg tcgcgggcca ccgtcacttc
     1081 gccccggcgc ttgccgttca cctggaccgc gatggtcacg gtgtcggttt tgagcagagc
     1141 gggatcgtgt ttgagccagg acgtttcggc gacgagcttg gtgtgaccca gcgcctgcca
     1201 gcattcttcc gccagatgcg gcatcatcgg cgcggataga agcaccagcg cttccaaggc
     1261 ttcgcgcctt gtggcggcat cggctttgga cgcatccgcg atggcgttcg atagcatgta
     1321 gagctgtgcc accgcgcggt tgaagcgaag cccgctcaaa tcttcggtga ccgcggcaat
     1381 cgccttgtgc gccgcctgac gcagcgcttt ggagacatca tcggcgggcg ctgcggcggt
     1441 gcccggcgcg ggcagacctt cggcttcgct cgccagacgc cagacgcgct gcacgaaacg
     1501 ccagcaccct tccgcgcccg cgtcggtcca ttcgatatcg cgttcgggcg gcgtgtccga
     1561 cagcatgaac cagcggatcg tgtcggcgcc atagtcggaa atgatcgctt cg
//
LOCUS       DASYEG010000073         1662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4080121, whole genome shotgun sequence.
ACCESSION   DASYEG010000073 DASYEG010000000
VERSION     DASYEG010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4080121"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VGM72_02780"
     CDS             <1..477
                     /locus_tag="VGM72_02780"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY4264215.1"
                     /translation="HSPIEQALTVRRGVCQDFAHIMIAVARGWGIPARYVSGYLYHRR
                     QDKDRSGDDATHAWVEAYLPSFGWVGFDPTNNIMASERHIRAAVGRDYDDVPPTRGTF
                     KGDADSELAVSVTVEPTSAPVRHEEFLRVARPMGAAAEAKPSAEQQNYYQQQQQQQ"
     gene            complement(535..>1662)
                     /gene="pcaF"
                     /locus_tag="VGM72_02785"
     CDS             complement(535..>1662)
                     /gene="pcaF"
                     /locus_tag="VGM72_02785"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: protein motif:HMM:TIGR02430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEY4264216.1"
                     /translation="DDLAAVPIQALMARNTDADWGALDEVILGCANQAGEDNRNVARM
                     AALLSELPESVPGATINRLCASGLDAVGTAARAIRTGEIDFAIAGGVESMTRAPLVMG
                     KAESAFQRSAEIFDTTIGWRFVNPLFKALYGADSMPDTAENVAEAHQISREDQDKFAL
                     RSQHRAGRAIESGFFAEEIVRVHAKDAKGKPATLERDEHPRPQTTLEDLTRLKAPFRD
                     PGTVTAGNASGINDGAAALTLAAEGALNRHGLRPRARVLGMATVGVAPRMMGIGPVPA
                     TEKLLARLGLKIADFDVIELNEAFAAQALAVLRGLGLPDDAAQVNPNGGAIALGHPLG
                     ASGARLVLTALHQLEKTGGKRALVTLCVGVGQGVSLALERV"
BASE COUNT          281 a          579 c          514 g          288 t
ORIGIN      
        1 cattcaccca tcgagcaggc cctgacggtg cgccgcggcg tctgtcagga cttcgcccat
       61 atcatgatcg ccgtcgcacg cggctggggc ataccggcgc gctatgtctc gggctatctg
      121 tatcatcgcc gccaggacaa ggaccgctcg ggcgacgacg ccacgcacgc ctgggtggaa
      181 gcctatcttc cgagtttcgg ctgggtggga ttcgatccca ccaacaatat catggcgagc
      241 gagcgccata tccgcgccgc cgtcggccgc gactatgacg acgtaccgcc cacgcggggc
      301 actttcaaag gcgatgccga cagcgagctt gcggtttcgg tcacggtgga gcccacctca
      361 gcccccgtgc gccacgagga atttctgcgc gtcgcccgcc cgatgggtgc ggcagccgag
      421 gcaaaacctt cggccgagca gcagaattat taccagcagc agcaacagca gcagtagacg
      481 ctagggccgc gatccgcaaa gcgcacgaag ctcagtgtgc ttgcgttttt ccttttacac
      541 ccgctccagg gccaggctga cgccttgtcc gacgccgaca cagagcgtga cgagggcgcg
      601 cttgccgccg gtcttttcga gctgatgcag cgccgtcagc accagccgcg ccccgcttgc
      661 ccctaacggg tggccgagcg cgatcgcccc accattcgga ttcacctgcg ccgcatcgtc
      721 gggcaatcca agcccgcgca gcacggcaag ggcttgagcg gcaaaggcct cgttcagttc
      781 gatgacatcg aaatccgcga ttttcaagcc gagccgcgcc agcagcttct cggtcgccgg
      841 cacaggaccg atccccatca ttcgtggcgc aacgcccact gtcgccatgc ccagcacgcg
      901 ggcgcgcggt cgcaggccat ggcggttcag cgcgccttcg gcggcgaggg tcagcgcggc
      961 agcaccgtcg ttgatgcccg aggcattgcc cgccgtcacc gtaccgggat cgcggaacgg
     1021 cgccttcagc ctggtcaaat cttcaagcgt ggtctgaggg cgcggatgct catcgcgttc
     1081 aagcgtcgcc ggtttgccct tcgcgtcctt ggcatgcacg cgcacgattt cctcggcaaa
     1141 aaatccgctc tctatcgcgc gacccgcacg gtgctggctg cgcaacgcga atttgtcctg
     1201 atcctcgcgg ctgatctggt gggcttcggc cacgttctcc gccgtgtccg gcatcgagtc
     1261 ggcgccataa agcgccttga aaagcggatt gacgaagcgc cagccgatgg tggtgtcgaa
     1321 gatttccgcc gaacgctgga aggcgctttc tgccttgccc atcaccagtg gcgcgcgggt
     1381 catggattcg accccgccgg caatcgcgaa gtcgatctcg cctgtgcgga tggcgcgcgc
     1441 ggccgtgccc accgcatcca gcccggaggc gcacagccgg ttgatggtcg cacccggcac
     1501 cgattcgggc agttcggaca acagcgccgc catgcgggcc acattgcgat tgtcctcgcc
     1561 cgcctgattg gcgcagccca ggatcacttc gtcgagcgcc ccccaatcgg catcggtgtt
     1621 gcgcgccatc agcgcctgaa tcggaaccgc cgcgagatcg tc
//
LOCUS       DASYEG010000074         9709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_602530, whole genome shotgun sequence.
ACCESSION   DASYEG010000074 DASYEG010000000
VERSION     DASYEG010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 9709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9709
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602530"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VGM72_02790"
     CDS             complement(<1..738)
                     /locus_tag="VGM72_02790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY4264217.1"
                     /translation="MRQFLLRGAAMLALSALTQISASAAELSAANQTKLIGAVEAYVP
                     RMSEVALQIWAMPEMGYLENNTTALLQGELKKAGFTVQSGVAGMPTSFIARTGTNDGP
                     VIAILAEMDSLPGLSNAAVPERKPIPGQSSGHACGHNLFGSGAVAAAIAVKKWMEETG
                     TKGQLRVYGTPAEEGGGGKSYMVSAGLFKDVDATIYWHPADANNASQDKNLANISAKF
                     RFHGVAAHAAAAPERGRSALDAVEVMNV"
     gene            883..2160
                     /locus_tag="VGM72_02795"
     CDS             883..2160
                     /locus_tag="VGM72_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264218.1"
                     /translation="MPAIAFAAAPIGWLEAVQENGTVTGWALDPDNPAQPLTVNFYID
                     GGPGTGKLLGTTVANAPRRDVNGATGFAGDHGFSWTAPAISDDRVHRLSAYALEPSGS
                     GGSNLTGSGVLLNVTENTILGTLNMAPGALEGAASIVDSFKGSPIVITTANAFAGAIF
                     SLTWNGKQFINNYDHGRELQSASFFDGYLECLNPTEAGSGDDGQKLTSTSRLLSLSAV
                     GNVLRTRTQMAFWIKPGGESGHSCGSAARAVNTTVLSSDILSKTVTIGFQGMPNVIEH
                     QITFTVTEPHASALFQAVVGFMPASFSKFWSYNPLTEKLEPLSDRSGYQRFPVVLSTA
                     DGSYAMGVYSPDEPQTPEMRYGRYKFSGGLGDATNTWICRFDETNIAAGDYNYRCYSI
                     IGTLNDVRESLSKLYSYFGAGARVPPGTPSSRQ"
     gene            complement(2172..3077)
                     /locus_tag="VGM72_02800"
     CDS             complement(2172..3077)
                     /locus_tag="VGM72_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264219.1"
                     /translation="MLEFALLRRAFGAGLLATAAALTLSPATAQDTPAKDGIPDFMAP
                     GTSWTMINSNATDYVNPASGAKPVTNDPKYPHIGNLQPGQKTERVADLNSPILQDWVK
                     PQMAKYNQEVIGGTIPFVATSLCYPGGVPAQLLVPTTVFFIQKPNEVIMIWERDQLVR
                     HVYLNQQHTKNPRPTWFGDSVGHYENGDTLVIDTIGFVEHPMSFVDNYRTPHTKQLHV
                     TERWKITNVPGGGRRGGATVAGVKGIEVTFTVEDPGAFTMPWGGMMRYRNEKGSLEEF
                     VCAENNGGHFAAEKYEMPVAKKPDF"
     gene            3213..4889
                     /locus_tag="VGM72_02805"
     CDS             3213..4889
                     /locus_tag="VGM72_02805"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4264220.1"
                     /translation="MAAPVSIIDTRYDQMFPTLGPADVARLRRFGELRAYADGDYLVH
                     AGDKGIGMFLVLSGEVVITRHCEFVPHGPIVTHHAGSFGGELAQLSGRPALVDAQAKG
                     AVEVVVIPPHQLRNVLVEEAELGERIMRALILRRVGLIQEGVGGPVIIGPAGNGDILR
                     LQGFLTRNAHPHQILDSGSDPDAKLLLERFHIAPGELPIVLCPGGQLLRNPSENEIAR
                     CIGLVGAMDSTKIYDTVIVGAGPAGLAAAVYAASEGLSVLVLDCRAFGGQAGASARIE
                     NYLGFPTGISGLALMARAYNQAQKFGVEMGIPNEVVSLQSGEEPGGRFSLLLANDEHV
                     TARTVVIASGARYRRLDIANLSEFEGSSVHYWASPLEGRLCAAQEVALVGAGNSAGQA
                     AVYLATQAAKVWMIVRGKSLEASMSRYLIDRIAAQPNIEVLTETVVSGLEGKGGALDA
                     IRWRHLQTAEETRLALRHLFLFIGADPNTDWLGQCGVALDSKGFVHTDGHARPLETSR
                     EGVFAIGDVRANSIKRVAAAVGEGAQVVATLHAFLASAGEEPAIANPGRA"
     gene            4889..5197
                     /locus_tag="VGM72_02810"
     CDS             4889..5197
                     /locus_tag="VGM72_02810"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEY4264221.1"
                     /translation="MADACPHEKTIREVTPSARGCEECLRIGSRWLHLRLCRTCGHVG
                     CCDSSPHRHATKHFHATRHPIIEGYDPPEGWGWCYIDEIMFDLSDRMTPQLGPIPRYY
                     "
     gene            5342..5938
                     /locus_tag="VGM72_02815"
     CDS             5342..5938
                     /locus_tag="VGM72_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY4264222.1"
                     /translation="MIKNHLPAVLIALIALAAPALAAETTSDPGPQREFHARMKVCEA
                     CHGQNGVPKTATIPIITGQQENYLAKQIHDFRSGDRKIEVMAWMADALSESELEPTVA
                     NFAKKTWPSRSAGAASVPPPRGVAVCQSCHQQNFLGAAQAEGMTTPRLAGQNYEYLVE
                     AMGRFADGERTNNADMVQIMKAISPADREAMARYLSSL"
     gene            6257..7321
                     /gene="soxC"
                     /locus_tag="VGM72_02820"
     CDS             6257..7321
                     /gene="soxC"
                     /locus_tag="VGM72_02820"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR04555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HEY4264223.1"
                     /translation="MTHYTPLQDYAGIITPASLHFMQQHSSELPEIDADQHRLTVHGM
                     VDRPLSFSMDDLKRLPSVSRVHFVECHANSSPGIHDADDPNMGLPVQFIHGMASCSEW
                     SGVPLSVLLNMAGVQKEASWLVSEGGDAGKFTHTLPLAKAMDDVFVAYNQNGEPLRRE
                     QGYPIRLLVPGWEGPFNVKYLKHIKVVDQPYMTWNESMNHSVPRPDLGGKSRWYHFQW
                     GPKSVITRPSAGMEMRRGYVQITGLAWSGAGMVKKVDISIDGGRNWKEAKLQGPIHSK
                     AHTRFTFDWAWDGQEAELQSRCTDETGDIQPSLAELSKHWDTPLEEWRKANKPRAIHF
                     NAIQPWKVARDGRITDAMFS"
     gene            7305..7877
                     /locus_tag="VGM72_02825"
     CDS             7305..7877
                     /locus_tag="VGM72_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY4264224.1"
                     /translation="MRCSLKLLVPAMILAAPLAALAAAPNYNVGTPLSQQEIQGFDRI
                     IGPEGKELPPGRGTSKEGAEIFAKRCEVCHGKNGQNGIVRRLVVGSPGKPYRGNFYGS
                     EANGPSYYPYPTIAWDYINRAMPASNPGSLKPDEVYALVAFLYYQNGIINENDVMDAK
                     TLPKVEMPNKNGFVPAKPVYPIDPKQPSWY"
     gene            complement(7923..8579)
                     /locus_tag="VGM72_02830"
     CDS             complement(7923..8579)
                     /locus_tag="VGM72_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264225.1"
                     /translation="MRDLNQRTTEGTPRRGFFGLAALSAFGLFGLATSAGAQPAPSDG
                     PDWPGTLKGRHRQLFDIYDINEGFPLGFANNFLAPNASATAVMIFRHKGLPYALGSAI
                     WAKYKVGETFKILDPETKAPAVKNPYFEPKPGVLANADTALDRLLAKGTVMGACGVAL
                     RGQSRRLAGNAGVTPDEAFKEWAANLIPGVTIIPSGTWGVNRAQEAGCTYCAGGVPSA
                     "
     gene            complement(8741..>9709)
                     /locus_tag="VGM72_02835"
     CDS             complement(8741..>9709)
                     /locus_tag="VGM72_02835"
                     /inference="COORDINATES: protein motif:HMM:NF036590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N C-terminal domain-containing
                     protein"
                     /protein_id="HEY4264226.1"
                     /translation="TRAFLMRRDTDAFNRWEAGQQLSTELLRGMIGAIQSGAAPQVDP
                     VYIEAIGGVLQRAHEDHAFTALMLIPPSESELALATTPIDPQAIHAARNALIRSVSDA
                     HGPALAELYRTLESKGAFSPDAVSAGRRSLRNAALRFLTAADDEKAAQLADAHYRAAT
                     NMTEMTAGLAALARTRSPLRDAAFGHFHDRFRNDPLVLDKWMGLQATSPQPDTVNRVR
                     ILMGHPVFSMKNPNRVRALVGAFALGNPLRFHDRSGAGYRLLREVVQGLDAINPQTAA
                     RMAGAFETWRRYDPERQGLMRAELEAIASGSGLSANLYEMATKMLG"
BASE COUNT         1846 a         2894 c         3055 g         1914 t
ORIGIN      
        1 cacattcatc acttcgaccg catcgagcgc ggaacgtccg cgttcgggcg ccgcggcggc
       61 atgggcggcc acgccgtgga agcggaactt ggcgctgata ttggcgaggt tcttgtcttg
      121 cgaggcgttg ttcgcgtccg ccggatgcca atagatggtg gcatcgacat ccttgaagag
      181 gcccgcgctc accatatagg acttgccgcc gccgccttcc tcggccggcg tgccatagac
      241 gcggagctgg cctttggtgc cggtctcctc catccatttc ttcaccgcga tggccgcggc
      301 gaccgccccc gaaccgaaca gattgtgccc gcaggcatga ccggaactct ggccgggaat
      361 cggtttgcgc tcgggcaccg ccgcgttgga caggccgggg agcgaatcca tttcggcgag
      421 gatcgcgatc accggcccgt cattggtgcc ggtgcgggcg atgaaggagg tcggcatgcc
      481 ggcaacgccg ctctgaacgg tgaagcccgc ctttttcagc tcgccttgga ggagcgcggt
      541 ggtgttgttc tcgagatagc ccatctccgg catcgcccag atctgcaagg ccacttccga
      601 catgcgcggg acataggcct cgaccgcgcc gatcagtttg gtctggttcg ccgccgacag
      661 ttcggccgcg ctggcggata tctgcgtcag tgcagagagc gcaagcatcg cagcgccgcg
      721 caaaaggaat tgccgcatca ccctcatcct cccatgcagt ccccacctct tcgacagagg
      781 acggggaaca gtcctggaac aatatatagg tttgtgtttt catagtgtat gggaccgacg
      841 ccgcgaagaa ttctcatttt tgcggcggcc gcatgtcttg gaatgccggc tattgccttt
      901 gcggcggctc cgatcggttg gcttgaggcg gtccaggaaa atggaaccgt aacgggttgg
      961 gcactcgatc ccgacaaccc ggcgcagccg cttaccgtca atttctatat cgatggcggt
     1021 ccggggaccg gcaagctcct cggaacaacg gtcgcaaatg cgccgcggcg tgacgtcaat
     1081 ggcgccacgg gatttgcggg cgatcatggc ttctcctgga ccgcccctgc gatctccgac
     1141 gatagagtgc atcggctcag tgcatatgcc ctcgagccat cgggaagtgg cgggtcaaat
     1201 cttacaggtt ccggagtgct tcttaatgtc acggagaaca caattcttgg aaccttgaac
     1261 atggcccccg gggctttgga aggcgcggca agcatcgtgg acagtttcaa aggttctccc
     1321 atcgtcatta caacggcgaa cgcgttcgcg ggtgctatct tctcactcac ctggaacggc
     1381 aagcagttca tcaacaatta cgaccatggc cgcgaattgc agtccgcgtc gttcttcgac
     1441 ggctatctcg aatgtctcaa tcccacggaa gctggatcgg gggatgatgg tcagaaattg
     1501 accagtacga gccgtctgct ttctctgtcg gcagttggaa acgttttacg cacgcgcacg
     1561 cagatggcct tttggataaa accgggaggt gaaagcgggc actcctgcgg ttctgcggca
     1621 agggccgtaa atacaacggt tctttcgtcg gatattttga gcaagaccgt cacgatcggt
     1681 tttcaaggaa tgccgaacgt tatcgaacat cagatcacgt tcacggtcac cgaaccccac
     1741 gcaagcgccc tgttccaggc ggttgtcggt ttcatgcccg caagcttctc caaattttgg
     1801 agctataatc ccttgacgga aaaactcgaa cctctgtccg atcgatccgg ttatcaacgt
     1861 tttcccgtcg ttctttcaac cgctgacggt tcctacgcga tgggcgttta ttcgcccgac
     1921 gaaccgcaaa ccccggaaat gcgatatgga cgctataagt tctcgggcgg ccttggcgat
     1981 gcgacgaaca cctggatttg ccgttttgat gaaaccaaca ttgcggccgg cgactacaat
     2041 tatcgctgtt attccattat cggcacgctg aacgatgttc gcgagtcact gagtaaactg
     2101 tattcatatt tcggggcagg cgctcgcgtc ccaccaggca cgccatcctc gcggcaatag
     2161 atttcctccg ttcagaaatc gggtttcttc gccaccggca tctcgtattt ctccgccgcg
     2221 aaatggccgc cattgttctc cgcacagacg aattcctcca gcgatccctt ctcgttgcga
     2281 tagcgcatca tgccgcccca gggcatggtg aaagcgcccg gatcttccac cgtgaaggtg
     2341 acttcgattc ccttgacgcc ggccacagtg gcgccgccac gccgtccgcc gccgggcaca
     2401 ttggtgatct tccatcgttc ggtgacatga agctgcttgg tatgcggcgt gcgataattg
     2461 tcgacgaagc tcatgggatg ctcgacgaaa ccgatcgtgt cgatcaccag cgtgtcgcca
     2521 ttctcgtaat ggccgacgga gtcgccgaac caggtggggc ggggattctt ggtgtgctgc
     2581 tgattgagat agacgtggcg cacgagctgg tcgcgctccc agatcatgat cacctcattg
     2641 ggcttctgga tgaagaacac cgtcgtgggc accagcaatt gcgccggtac gccgccggga
     2701 taacacagcg aggtcgccac gaaggggatc gttccgccga tcacctcctg attgtatttc
     2761 gccatttgcg gcttcaccca atcttgcagg atcggactgt tgaggtcggc gacgcgctcg
     2821 gtcttctgcc ccggctgcag attgccgatg tggggatatt tcggatcgtt ggtcacgggt
     2881 ttggcgccgg aagcaggatt cacataatcg gtggcgttgg aattgatcat ggtccagctc
     2941 gtgccgggtg ccatgaaatc gggaatgccg tccttggcgg gcgtgtcctg tgcggttgcc
     3001 ggagaaagcg tgagcgccgc cgcggtggcc aggagaccgg cgccgaacgc gcgccgcaac
     3061 aaagcaaatt ccaacatcag acgttccttc cggatgagat ggcttgaatg gggatggcgc
     3121 caggatagtg cctgtcgcgc gacttgtcag cgtttcgcga agatggtctt taagacgtga
     3181 gcacgaaatg ctgtccttcc cgagagatgc ccatggccgc tcccgtctcg atcatcgata
     3241 cccgttacga ccagatgttt cccacgctgg ggcctgcgga cgttgcgcgt ttgcgccggt
     3301 tcggcgaatt gcgggcctat gcggatggcg attatctggt gcacgcgggc gataagggca
     3361 tagggatgtt cctggtcctc tcgggcgaag tcgtcatcac ccggcactgc gaattcgtgc
     3421 cccatggtcc gatcgtcacc catcatgcgg gttccttcgg cggcgagctg gcgcagctct
     3481 ccggccgccc ggcgctcgtg gatgcgcagg ccaaaggcgc ggtcgaagtg gtcgtcatcc
     3541 cgccgcatca gcttcgcaat gtgctggtcg aagaagccga attgggcgaa cggatcatgc
     3601 gggcgctgat cctgcgccgc gtcggtctga tccaggaagg cgtgggcggg ccggtcatca
     3661 tcggccccgc tggcaatggc gacattctgc ggctgcaagg gttcctgacc cgcaacgccc
     3721 atccccacca gatattggac tccggtagcg atccggacgc gaagctcctg ctggagcgct
     3781 ttcatatcgc gccaggcgaa ttgccgatcg tgctgtgtcc cggcggtcag cttctgcgca
     3841 atccgagcga gaacgaaatt gcccgctgca tcggcctcgt gggcgcgatg gattccacca
     3901 agatttatga cacggtcatc gttggggccg gcccggccgg actggcggcg gcggtctacg
     3961 cggcgtccga agggctttcg gtgctggtgc tcgattgccg cgctttcggc ggacaggcgg
     4021 gcgcgtccgc gcggatcgag aattatctgg gcttcccaac gggaatctcc ggcctcgctt
     4081 tgatggcgcg ggcctacaac caggcccaga aattcggcgt cgagatggga attccgaatg
     4141 aagtcgtgag tttgcagagt ggggaggaac ccggcggacg gttttccctg ctgctcgcca
     4201 atgacgaaca cgtcaccgcg cgaaccgtgg tgatcgcgag cggcgcacgg taccggcggc
     4261 tcgatatcgc aaatctctcc gagttcgagg gttcttccgt gcattattgg gcgtcgccgc
     4321 tggaggggcg gctctgcgcg gcgcaggagg tcgccctggt gggtgcggga aattccgcgg
     4381 ggcaagccgc ggtctatctg gcgacacagg ccgcgaaagt ctggatgatc gtgcgtggga
     4441 aaagtttgga agccagcatg tcgcgttatc tgatcgaccg catcgccgcg cagcccaata
     4501 tcgaagtcct gacggagacg gtggtgagcg ggctcgaagg caagggcggt gctctggacg
     4561 ccatccgctg gcgccatttg caaacggccg aggagacccg gctggcgttg cggcatctgt
     4621 tccttttcat cggcgcggac ccgaataccg actggctggg acaatgcgga gtggcccttg
     4681 acagcaaggg cttcgtgcac accgatggcc atgcgcgtcc gctcgaaacc agtcgcgaag
     4741 gcgtcttcgc catcggcgac gtgcgcgcca attccatcaa gcgggttgcc gccgcggtcg
     4801 gcgagggggc gcaagtggtg gcgacgcttc atgccttcct tgccagcgcg ggcgaggaac
     4861 cggcgatcgc caatcccggg agggcctgat ggccgatgcg tgcccccatg agaagaccat
     4921 acgcgaggtc acgccgagcg cgcgcggctg cgaggagtgc ctgcgcattg gttcacgctg
     4981 gctgcatctg cggctctgcc ggacctgcgg tcatgtcgga tgctgcgatt catcgccgca
     5041 tcgccacgcc accaagcatt ttcacgcgac ccgccatccg atcatcgaag gctacgatcc
     5101 ccccgaagga tggggctggt gctatatcga cgagatcatg ttcgatcttt cggaccgcat
     5161 gacgccgcag ctcggaccga tcccgcgcta ttattgaaag gcctttcgtc cccggcccag
     5221 cagagccatc cgaaaacccg ccatcttgcg ccccaagcct gactgcggca tgttccacgg
     5281 gttccagcgc gttggtcgcc tctcaaaaat ttcaggcagt cgaacaatgg cagggccaaa
     5341 catgatcaaa aatcatcttc cggctgtttt gatcgctctg atcgcgctgg ccgcgccggc
     5401 acttgccgcc gaaaccacct cagatccggg cccgcaacga gaattccatg cgaggatgaa
     5461 agtttgcgag gcatgccacg gccagaacgg cgtgcccaaa actgcgacca ttccgatcat
     5521 cacggggcag caggagaatt atctcgcaaa gcagatccat gacttccgca gtggagatcg
     5581 caagatcgag gtcatggcgt ggatggcgga cgctctctcg gagtcggaat tggagcccac
     5641 ggtggctaat ttcgcaaaga agacatggcc gtcgcggtcc gccggtgccg cttccgtgcc
     5701 gccgccgcgc ggcgtggctg tatgccagag ctgtcaccag cagaacttcc ttggcgccgc
     5761 tcaggcggaa ggcatgacga cgccgcgcct ggccggccaa aactacgaat atctcgtcga
     5821 ggcgatgggc cgctttgccg atggcgagcg gaccaacaat gccgacatgg tgcagattat
     5881 gaaggcgatt tcgcctgccg accgcgaagc gatggcgcgt tatctctcca gcctctgacc
     5941 ggccggccgc tcataatggc ttcgagttga ctcgctgttg gttttacaaa acaatttaga
     6001 gttgtgcgta gacgagaaac gttcgggcca agacaaagct caaatcgcaa cgggaccacg
     6061 ggaggaaatc atggattcca atggatctgg ccgcagacgg ttcctgaaac aaacggctgc
     6121 gatggccggg gccggtttgg ccggaagcgc ggtatggtct gggaaaggcc aatccgccga
     6181 gactcaagca ccgccccagg ctggccacgt ccacaatgaa cccccgaggc ggggggcccg
     6241 gtacaccatc gatcacatga ctcactacac cccgcttcag gactatgcgg gaatcatcac
     6301 gccggcctcg ctccacttca tgcagcagca ctcctccgag ctgcccgaga tcgatgcgga
     6361 ccaacatcgc ctcacggtcc acggcatggt ggatcgtcct ttgagcttca gcatggacga
     6421 tctgaagcgg ctgccttccg tatcccgggt tcatttcgtg gagtgccatg caaacagctc
     6481 tccgggaatc catgatgccg acgatccgaa catggggttg ccggtccagt tcatccacgg
     6541 gatggcgagt tgcagcgaat ggagcggcgt gccgctttcg gtgttgctga acatggccgg
     6601 cgtgcaaaaa gaggcgagct ggctggtttc ggaaggcggg gacgccggca agtttacgca
     6661 cacccttccg ctggcaaagg ccatggacga tgtcttcgtg gcctataacc aaaatggcga
     6721 gccgctgcgc cgtgaacagg gttatccgat ccggctgctc gttcccggct gggaaggccc
     6781 tttcaacgtg aagtatttga agcatatcaa ggtcgtggat cagccttaca tgacttggaa
     6841 cgagagcatg aatcactccg tcccgagacc cgatctgggc ggcaaatcgc gctggtacca
     6901 ttttcagtgg gggccgaaat cggtgatcac gcgcccctcg gccggaatgg aaatgcgccg
     6961 gggatatgtc cagatcaccg gcttggcctg gtccggtgcg ggcatggtca agaaggtcga
     7021 tatctccatc gacggcggcc gcaattggaa agaagccaag cttcaggggc caatccactc
     7081 aaaggcgcat acacggttta ctttcgactg ggcttgggac ggacaggaag cggaactcca
     7141 gtctcgctgc acggacgaaa cgggcgacat tcagccgtcg ctggcggagc tgagcaagca
     7201 ttgggacacc ccgctcgagg aatggcgcaa ggccaacaag ccccgcgcca ttcatttcaa
     7261 tgccattcag ccctggaaag tggcccgcga cgggaggatc accgatgcga tgttctctta
     7321 aattgcttgt gccggcgatg atcctggcag cccctctggc cgcgctggcg gcggcgccca
     7381 actacaatgt gggcacaccg ctgagccagc aggaaatcca gggttttgac cgtataatcg
     7441 gtccggaggg aaaggaactt ccgcccgggc gcggaacctc caaggaaggt gcggagattt
     7501 ttgcgaagcg ctgcgaggtg tgccacggga agaatgggca aaatgggata gtgcggcgtt
     7561 tggtggtcgg cagtcccgga aaaccttatc gcggcaattt ttacggttcg gaagcgaacg
     7621 ggccaagcta ctacccgtat ccgacgatcg catgggacta tatcaaccgt gcgatgcccg
     7681 cgagcaatcc gggatcgctc aagccggatg aagtgtacgc gctggtcgcg tttttgtact
     7741 accagaacgg catcatcaat gagaatgacg tcatggatgc caagaccttg ccgaaggtcg
     7801 aaatgcctaa caagaacggc ttcgttccgg caaagccggt ttatcccata gatcccaagc
     7861 aacccagttg gtattgagcg ccaacggcgg cgggcggggg cgacgccgct cccgcgattt
     7921 cctcaggcgg acggcactcc tcccgcacaa taggtgcagc cggcttcctg cgcccgattg
     7981 acgccccatg tccccgaagg aatgatcgtg acgccgggga tcaagttggc ggcccattcc
     8041 ttgaacgctt cgtccggggt tacgccggcg tttccggcaa gcctgcggct ctggccgcgt
     8101 aatgcaacac cgcacgcgcc catcaccgta cctttggcga gcagtctgtc gagagcggtg
     8161 tcggcgttcg ccagcacgcc gggcttcggc tcgaaatagg gattcttcac ggcgggtgct
     8221 tttgtttccg ggtcgaggat tttgaatgtt tcgccgacct tgtatttggc ccaaatcgcg
     8281 ctgccgagtg cgtagggcag ccccttgtgc cggaagatca tgacagccgt cgccgacgca
     8341 ttcggcgcga gaaaattgtt cgcgaagccg agcggaaatc cctcgttgat gtcgtagatg
     8401 tcgaaaagct gccggtggcg gcctttcagc gttcccggcc aatccgggcc gtccgatgga
     8461 gccggttggg cgcctgcgct ggtggctaaa ccgaacaggc cgaacgccga tagggcggca
     8521 aggccgaaga atccgcggcg gggtgtgcct tcggtcgtac gctgattgag atcgcgcatc
     8581 taatcctcca tcgcgtgccg ctcgcacgct tttgcgtggt ggtgccccca acgcgcgcca
     8641 gccttgttcg cggccggttg gcgccagcgt aacacttcca tcggcaagcg gttaaggggg
     8701 caatggtccg gcgtatggct tatttagggg ggagtgaaaa ctaccccagc atcttggtgg
     8761 ccatctcgta gagattggcc gaaaggccac tgcccgaagc aatggcttcg agttcggcgc
     8821 gcatcagacc ctggcgttcg ggatcgtaac gcctccaggt ctcgaacgcg ccggccattc
     8881 gcgcggcggt ctgtggattg atggcatcga gaccctgcac cacctcgcgc agcaggcgat
     8941 agcccgcgcc gctgcggtcg tggaagcgca acgggttgcc cagcgcaaag gcacccacca
     9001 gcgcacgcac gcgattggga ttcttcatgg agaagaccgg gtgccccatc aggatacgca
     9061 cgcggttgac ggtgtcgggc tgcggcgacg tcgcctgcag acccatccat ttgtcgagca
     9121 ccagcggatc gttgcggaag cggtcgtgga aatggccgaa cgccgcatcg cgcaaggggc
     9181 tccgcgtgcg ggcgagtgcg gcgaggccgg ccgtcatctc ggtcatgttg gtcgcggccc
     9241 gataatgcgc atcggcaagc tgcgcggcct tttcgtcgtc ggccgcggtc aggaagcgca
     9301 acgcggcgtt gcgcaaggaa cgccgcccgg ccgacaccgc atccggactg aacgcgccct
     9361 ttgattccaa cgtgcgatag agttccgcca gtgctggacc atgcgcgtcc gagacgctgc
     9421 ggatgagtgc gttgcgcgcg gcatgaatgg cttgcggatc gatcggcgtc gtggcgagcg
     9481 caagttcgct ttcgctcggg ggaatgagca tcagcgcggt gaaggcgtgg tcttcgtggg
     9541 cgcgctgcag aactcctccg atggcctcga tatagacggg atcgacctga ggggctgcac
     9601 ccgattggat ggccccgatc atgccgcgca gcaattcggt ggaaagctgc tggcctgctt
     9661 cccagcggtt gaacgcgtcg gtgtcccggc gcatcaggaa ggcgcgcgt
//
LOCUS       DASYEG010000075         2631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3356693, whole genome shotgun sequence.
ACCESSION   DASYEG010000075 DASYEG010000000
VERSION     DASYEG010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3356693"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..463
                     /locus_tag="VGM72_02840"
     CDS             59..463
                     /locus_tag="VGM72_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY4264227.1"
                     /translation="MNRLMVPAVLLASGVAATAAWSQPKPPAMQNNRYFTMPLEKDPT
                     REVGLQSVTMPPGAGNEFHRHPGDQWTAVQEGEVTFTVKGKPPQVLKAGDYNYVPRGT
                     VHRQQNLSGKPARYIEMRIFDKGKPASEAVAD"
     gene            complement(500..877)
                     /locus_tag="VGM72_02845"
     CDS             complement(500..877)
                     /locus_tag="VGM72_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264228.1"
                     /translation="MKFSGIIVTATVLAFAGYAIAQTEKTPENQAAPDWITTSNKPCK
                     VWNPEPQPNESVTWSGTCKDGLASGKGILFWTEDGKPDVEFDGEYANGKRNGHGVVIS
                     PDGNMFEGDWVDDKLITNARDSI"
     gene            1071..1979
                     /locus_tag="VGM72_02850"
     CDS             1071..1979
                     /locus_tag="VGM72_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY4264229.1"
                     /translation="MKKFAWLLVGALVPIAAMAADAPGPGWAFMTPDPNAPAPAAGGG
                     GGGGGGGGGGAPAAPVAQPGVPQIVVAGKGAEVRPCNTCHTPSGMGQPESANIRGLNA
                     EYFARQMADFKSGARGGPRSAAMATFAKGLSDDEVKEIAAYYAGLKPMQWTRMTEANV
                     VPKTVVGRNSQRTRLEGSETEQVGNRIIEFASSPAAVRQMAEPAFVAFAPPGALGNGK
                     NLVTTGGAGKTQACSGCHGDKLQGEDDVPGIAGHSPVYIARQIYSFKNGVRKGPLAEI
                     MKGVIGKLSDDDVIGVASYVSSLDPA"
     gene            complement(2058..>2631)
                     /locus_tag="VGM72_02855"
     CDS             complement(2058..>2631)
                     /locus_tag="VGM72_02855"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264230.1"
                     /translation="KGNDVFDRPTGIAFAPNGDIFVSEGHAPTFGNSRITKFDKNGKF
                     IKSFGHLGSGDGELKGPHVLAFDSQGRLFVADRSNSRVAIFDQDGKFLAAWKQFGRPS
                     GIFIDKNDVLYVSDSESQDAPGKDTNNPGCKRGIRVGSAKTGKVDFYIPPPTPKNPKN
                     PPPEGVAADSHGNIYAAAVPEKSVYKYVRN"
BASE COUNT          500 a          791 c          833 g          507 t
ORIGIN      
        1 cccttcagag ccacgcgtaa accatgtaag cttcttccat cacctccggg agttattcat
       61 gaacagattg atggtgccag cggtgctcct ggcaagcggt gtcgcagcaa cggccgcatg
      121 gtcgcagccg aagccgcccg cgatgcagaa caatcgctat ttcacaatgc cgctggagaa
      181 agacccgacg cgcgaggtgg gtctgcaatc cgtcaccatg ccgccgggcg ccggcaacga
      241 attccaccgc catcccggcg atcagtggac tgcggtccag gaaggcgaag tcacgttcac
      301 cgtcaagggc aaaccgccgc aagtgctcaa agcgggcgac tacaattacg ttccgcgcgg
      361 gaccgtccac cgccagcaga atctctccgg caagcctgcg cgctacatcg agatgcgaat
      421 cttcgacaag ggcaagccgg cgtcagaagc ggtggcggac tgacctataa cgtaacggca
      481 tcatgcgcgc gcgcgctgct cagatcgagt cgcgcgcgtt cgttatgagc ttgtcgtcga
      541 cccagtcgcc ctcgaacatg ttcccatcag gcgagatcac gacgccgtgg ccgttgcgct
      601 tgccgttggc gtattcgcca tcgaattcca catcgggttt gccatcttct gtccaaaata
      661 aaatcccctt ccccgatgcg aggccgtcct tgcaggtgcc cgaccaggtc acggattcgt
      721 tcggctgggg ttcggggttc caaaccttgc acggtttgtt ggacgtcgtg atccaatccg
      781 gagcggcctg gttttctggc gttttttcgg tctgtgcgat ggcgtacccg gcgaaagcca
      841 ggacagtagc agtgacgatg atgccggaaa atttcatcct tccacctcaa cgatacgaaa
      901 caatcagctt agcaaactct gcgcatgcgt gccatcgcgg ggcacgcgca tctcgggatt
      961 tcacgtccgc ttgcagcgcg gggcgtgggg gtcccttgcc gcttcggtgc actcacaata
     1021 ttgtcactgc aagcgtgccc cggatgccgg gcggcgtaac ggaggggacc atgaagaaat
     1081 ttgcatggct gcttgtaggc gcattggttc cgatcgcggc tatggcggcg gatgctccgg
     1141 gtcctggttg ggccttcatg actccggacc cgaacgctcc ggctcctgcc gccggtggcg
     1201 gaggaggcgg cggcggtggc ggtggcggcg gtgcacccgc agcgcctgtg gcgcagcccg
     1261 gcgttcctca gatcgttgtg gcgggcaaag gcgccgaagt gcgcccctgc aacacctgcc
     1321 atacgccttc cggtatgggc cagccggaat cggcgaatat ccgcggcctg aacgccgagt
     1381 atttcgcgcg gcagatggcg gacttcaaaa gcggggcgcg cggcggtccg cgatcggcag
     1441 ccatggcgac cttcgccaaa ggcctctccg acgatgaggt gaaggaaatc gccgcctatt
     1501 atgccggatt aaagccgatg caatggacgc gcatgaccga agccaatgtc gtgcccaaga
     1561 ccgtggtcgg acgcaacagt cagcgcaccc ggctggaagg gtcggagacc gagcaggtgg
     1621 gcaatcgcat catcgaattc gccagcagcc cggcggccgt gcggcaaatg gcggaacctg
     1681 ctttcgtcgc gtttgcgcct cccggtgcgc tcggcaatgg aaaaaatctg gtgacgaccg
     1741 gcggggccgg taagacgcag gcttgctccg gctgccacgg cgacaagctg caaggcgagg
     1801 acgatgtgcc gggcattgcc ggacattcgc cggtctacat cgcgcgccaa atctattcct
     1861 tcaagaacgg cgtgcgcaaa ggaccgctgg ccgagatcat gaagggcgtc atcggcaaat
     1921 tgtccgatga cgatgtgatc ggggtggcgt cttacgtgtc ctcgctcgat ccggcctgac
     1981 cgggcggagt ggcggaaaaa caaaatgccc gcggggagtg acctccccac gggcgttttc
     2041 gtttgcccaa aacgggctca gttcctgacg tatttgtaga ccgacttctc cggcacggca
     2101 gcggcgtaga tgttgccgtg ggaatcggcg gcgacacctt ccggcggcgg gttcttcgga
     2161 ttcttcggtg tcggtggggg aatgtagaaa tcgaccttgc cggtcttggc gctgcccacc
     2221 cggatgccgc gcttgcagcc gggattgttg gtgtccttgc cgggcgcatc ctgcgattcg
     2281 gaatccgaaa cgtagagcac gtcgttcttg tcgatgaaga ttccgctcgg cctgccgaat
     2341 tgcttccacg cggcgaggaa ttttccgtcc tgatcgaaga tcgcgacgcg gctattgctg
     2401 cggtccgcaa cgaacaagcg cccctggctg tcgaaggcca gaacatgggg acctttcaat
     2461 tcaccatcgc cagagcccag atggccgaaa ctcttgatga acttcccgtt cttatcgaat
     2521 ttggtgatgc gcgaattgcc aaaggtcggc gcatggcctt cggagacgaa aatgtcaccg
     2581 ttcggggcaa aggcgatgcc ggtcggccgg tcgaagacgt cattgccctt g
//
LOCUS       DASYEG010000076         8629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4516723, whole genome shotgun sequence.
ACCESSION   DASYEG010000076 DASYEG010000000
VERSION     DASYEG010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 8629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8629
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4516723"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VGM72_02860"
     CDS             complement(<1..374)
                     /locus_tag="VGM72_02860"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036535.1"
                     /note="Catalyzes the reversible phosphorolysis of
                     5'-deoxy-5'- methylthioadenosine (MTA) to adenine and
                     5-methylthio-D-ribose-1- phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEY4264231.1"
                     /translation="MTKTILGIIGGSGVYDISGLENARWQHVDSPWGEPSDDLLLGTL
                     SGVDMAFLPRHGRGHVHSPSSINYRANIDALKRVGCTDVISVSACGSLRENFPPGHFV
                     LPDQFIDRTHAREKSFFGRGFVA"
     gene            complement(476..1300)
                     /locus_tag="VGM72_02865"
     CDS             complement(476..1300)
                     /locus_tag="VGM72_02865"
                     /inference="COORDINATES: protein motif:HMM:NF014247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEY4264232.1"
                     /translation="MRNSVTISFLAAALFSALALPVLALDKPASPPSTEPLPPKKMAW
                     SFSGPFGTYDRAALQRGFQVYKEVCSNCHSLTRVALRNLADAGGPGFSPEQVAALAAG
                     YKVPAEPNAQGKTTDDMGQPLTRAGVASDYFPPPFPNEKATRAAMNGALPPDLSLIVK
                     AREGHEDYVYSILTGFGQKPPANEKMARGMNYNPYFPRHQIAMPPPLTNGTVTYADGT
                     PNTIDQEAHDVVTFLAWTSEPKMEERKRMGFNVILFLAAFSTLLYFTYRRVWHGEH"
     gene            complement(1312..2577)
                     /locus_tag="VGM72_02870"
     CDS             complement(1312..2577)
                     /locus_tag="VGM72_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEY4264233.1"
                     /translation="MSGPSTYEPKTGFERWLDVRLPIIRFSQEHLASYPTPRNLNIWY
                     TFGGILSAFLVTQILTGIVLAMHYTPDSRLAFDSVEHIMRDVNSGWLLRYLHSNGASF
                     FFLAVYIHIFRGLYYGSYKAPREIIWILGVVLYILMMATAFLGYTLPWGQMSFWGATV
                     ITNFFGAFDALIPGLGTGIVTWLWGGFGVDNATLHRFFSLHYLLPFAIAGVVALHIWA
                     LHVPGNNNPLGIDVKSPQDTVSFHPYYTVKDLFYLSCFLTIYAYFVFFNPNMLGDPIN
                     YAPANPIQTPEDIVPEWYFLPFYAILRSIPNKLIGVLALFGSLLTLFAVPWLDTSPVR
                     SSRFRPLYRQFFWMLFADCLILGYVGSQPVDASLFNTAVPMVWLGRIATAYYFLHFWI
                     VMPIVGLIETPKPLPDSIAKSVLATAPAE"
     gene            complement(2589..3176)
                     /gene="petA"
                     /locus_tag="VGM72_02875"
     CDS             complement(2589..3176)
                     /gene="petA"
                     /locus_tag="VGM72_02875"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008035832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEY4264234.1"
                     /translation="MDHSQTAEPTRRDFLYIATAALGAVGAAATLIPLIDQMNPSADV
                     LALASIEVDLAPIMAGQSVVFTWRSHPLFVRRRTPEEIAAARAVKMSDLIDPVARNDS
                     LPDSAPATDENRIKPATGAVKPEWLVLVGVCTHLGCTPEASTPQSPQGDYGGWLCHCH
                     GSQYDTAGRVRVGPAPRNLEVPVFAFLSDTRIKVG"
     gene            complement(3402..3815)
                     /locus_tag="VGM72_02880"
     CDS             complement(3402..3815)
                     /locus_tag="VGM72_02880"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY4264235.1"
                     /translation="MNTPLLLDTCAAIWAAEDQLAPAAADLLTQSYRRGEPVYLSPIT
                     AWELGLLFSRGRLRAARSPLDYFRHLINLPGVRLAAMPPELLLASSFLPDSPPSDPAD
                     RIMATTAREYGYTLMTRDDRLLAYAEQGHIRAFAC"
     gene            complement(3812..4285)
                     /locus_tag="VGM72_02885"
     CDS             complement(3812..4285)
                     /locus_tag="VGM72_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264236.1"
                     /translation="MSKYNSLHGFLNTQAASELPMTFGEIEQVIGSKLPPSAYRHRPW
                     WANEAANHVHAKAWLSAGYRTEQVDMAGKKLIFKRAHFPSPPSGGPAAESRGGLGEPM
                     RAFQDDSAKNPAARHPLIGALKGWLIVEPGYDLTQPAMPEWAELADRKYGRENPQ"
     gene            4350..4814
                     /locus_tag="VGM72_02890"
     CDS             4350..4814
                     /locus_tag="VGM72_02890"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEY4264237.1"
                     /translation="MRLCLFEPDIPQNAASLIRLGACLGVAVDIVEPCGFLFSESGFR
                     RAGMDYVERADLVRHRSWEEFRATAQGRLILLTTKAPLPYTAFAFQDGDILILGRESA
                     GVPDHVHDAAEARLRIPLRSGLRSINVAQAGALVLGEALRQTNGFPAEGLPG"
     gene            4811..5704
                     /gene="hemF"
                     /locus_tag="VGM72_02895"
     CDS             4811..5704
                     /gene="hemF"
                     /locus_tag="VGM72_02895"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF003727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEY4264238.1"
                     /translation="MNVPNAEVPSIAARKEAAASWFRALRDKICAAFEAIEDDYTGAP
                     ASAPGRFVRKDWKRPTADGSDGGGGTMALMKGRVFEKVGVNISTVHGRFEPQFAKEIP
                     GADKDPSYWASGISLVAHMHSPRVPTAHFNTRHIVTGKSWFGGGGDLTPMLMEQRNAE
                     HRNTVAFHAAFRRACDAHGPDYYARFSKWCDEYFTIKHRGETRGVGGIFYDYLESDWE
                     KDFAFTQDVGRAFLAAYPAIIRDSMNEAWTRAEKDEQLVRRGRYAEFNLVYDRGTRFG
                     LMTGGNAEAILMSLPPEVRWP"
     gene            5756..6403
                     /locus_tag="VGM72_02900"
     CDS             5756..6403
                     /locus_tag="VGM72_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03717.1"
                     /note="Members of this highly hydrophobic protein
                     family,regularly are found preceded by the yybP-ykoY
                     manganese riboswitch (see RF00080). A metal cation
                     transport function is proposed; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjbE family putative metal transport protein"
                     /protein_id="HEY4264239.1"
                     /translation="MDLFSSSALFALAQVLMINIVLSGDNAVVIGMVAARVEPGNRRN
                     VILWGMVAAVVLRVLLALVAVDLLNILGLLLAGGIVLLWVAWRLYRDIREQHSEKAAL
                     KAKESRGESSQGAVSASSMSLRNAIWQIAVADISMSVDNVLAIAGAAKQNIPVLVIGL
                     AVSVALMGVAATYIARLLQRFPALSYIGVALIVYVGAEMIWEGSGDVPEILKFFR"
     gene            complement(6426..7361)
                     /locus_tag="VGM72_02905"
     CDS             complement(6426..7361)
                     /locus_tag="VGM72_02905"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4264240.1"
                     /translation="MKRAGLAAFGAAAILQTEEGRAQVAVSNSTGTESPKLKAPANAA
                     DCHMHIYNPAHFPLAPSPRVAPENAAVDQYRLLQKRIGTTRVVIVTPRNYATRNDATV
                     DAIAQLGANARGVAVLHPDVSDAELKRLNAAGIRGTRFSLGDPSTAVVMPDMVEPMAR
                     RVAPLGWHLQMNMGGEQIVAMADLLKRLPAPLVFDHLGIPPLPAGVTHPSHAIVRGLL
                     DSGRAWVKLSGAYLNSKVGPPYPEASRIAQDFVKAAPERLVWGSDWPHPTSPGPAKPD
                     DALLFDLLAVWAPDERTRQRILVENPQNLYGFTKV"
     gene            complement(7458..>8629)
                     /locus_tag="VGM72_02910"
     CDS             complement(7458..>8629)
                     /locus_tag="VGM72_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4264241.1"
                     /translation="VLRRLQRWQDALACIDTALSIEPGSAEALSSRAAILAEASRPAE
                     SLATLDRMLALWPDDAAGWNNRGSVLVSMSRLDDALASFDRALAIQPDFIEALSNRAV
                     MLLQLFRLDEAIAACDAAVRLRPDHAISWNNRGNILMALRRYQEAATSYDKALEIDPA
                     LAATAENRDLAMLKLKRLTRCPPGYVRGLFDEFSSDYDQIMLETLGYRAHLHLRTLAN
                     RVLGSGAGALRILDLGSGTGLVGDAFKDLAKGGRLDGIDLSPRMIDEASRRGIYDNLI
                     LGDIETMLLAVERRYDLVLAADTMIYFGDLAPTLSGVYQRLVPGGLYLFAVERMEGHG
                     WEQTEDKRFRHGEAYLRSSARYAGLEVADLMTCVLRRQASEPVPGLAVAVRKPPA"
BASE COUNT         1750 a         2587 c         2731 g         1561 t
ORIGIN      
        1 gcgacgaagc cgcggccgaa aaagcttttc tcgcgcgcat gggtgcggtc gatgaactga
       61 tcgggcagaa cgaaatggcc gggcggaaag ttctcgcgca aggaaccgca agccgaaacc
      121 gagatgacat cggtgcagcc cacgcgcttc agcgcatcga tattggcgcg gtaattgatc
      181 gacgagggcg aatgcacatg gccgcgcccg tgccggggca gaaaggccat gtcgacgcct
      241 gagagtgtcc caagcagcaa atcgtcggac ggttcgcccc agggagaatc cacatgttgc
      301 cagcgcgcat tttcaaggcc cgagatgtca tagacgccgg agccgccgat gatgccgaga
      361 attgttttgg tcatggatga ttcgtaactg ccagagaata tcgcaaaacg aagatagcct
      421 cgttccgaga cttcgcgcca cctccgcggg cactatttgc gaaaggccgc gggcttcagt
      481 gctcgccgtg ccagacgcgg cgataggtga aatacagcag cgtcgaaaac gcggccagga
      541 acaggatcac gttgaagccc atgcgcttgc gctcttccat cttgggctcg gacgtccagg
      601 cgaggaacgt caccacgtca tgggcttcct gatcgatggt gttcggcgtg ccgtcggcat
      661 aggtgaccgt accgttcgtc agaggcggcg gcatggcgat ctgatgacgt ggaaaatatg
      721 ggttgtagtt catgccccgc gccatctttt cgttcgccgg cggcttctga ccgaagccgg
      781 tgaggatcga atagacgtaa tcctcatggc cttcgcgcgc tttcacgatc agggacaaat
      841 ccggcggcag ggcgccgttc atggccgcgc gcgtcgcctt ctcattgggg aagggcggcg
      901 gaaaataatc cgacgctacg ccggcgcgcg tcagcggctg ccccatgtcg tcggtggtct
      961 tgccctgcgc gttgggttcg gcgggcactt tgtagccggc cgcgagtgcg gccacctgtt
     1021 ctggtgagaa gcccgggccg cccgcatcgg cgagattgcg cagcgcgacg cgggtgagcg
     1081 aatggcaatt ggagcagact tccttgtaga cctggaaacc ccgctgcagg gcggcgcggt
     1141 cataggtgcc gaacgggccg ctgaacgacc aggccatctt cttggggggc aatggctcgg
     1201 tcgagggagg agacgccggt ttgtccagcg caagaacagg aagggcgagc gcactgaaaa
     1261 gtgctgcggc aagaaacgaa attgtcacgg aattgcgcat cttcgttttc cctactcggc
     1321 cggcgcggtg gcgagcaccg atttggcgat ggagtcgggc agaggcttgg gcgtctcgat
     1381 caatccgacg atgggcatga cgatccagaa atgcaggaaa taataggcgg tggcgatgcg
     1441 ccccagccac accatgggaa cggccgtatt gaaaagggac gcgtccacgg gttgcgaccc
     1501 gacatagccg aggatcaaac aatccgcgaa cagcatccag aagaactggc gatagagcgg
     1561 acgaaaccgc gacgagcgca ccggcgaagt gtccagccag ggcaccgcaa acagcgtcag
     1621 caacgaaccg aacagcgcca gcacgccgat gagcttgttc gggatcgagc gcaaaatcgc
     1681 gtagaacggc aggaagtacc attccggaac gatgtcttcc ggcgtctgga tcggattggc
     1741 cggcgcgtaa ttgatcggat cgcccagcat gttgggattg aagaacacga aataggcata
     1801 aatggtcagg aagcaggaca gataaaacag gtccttgacc gtgtaatagg ggtggaagga
     1861 caccgtgtcc tgcgggctct tcacgtcgat gccgagcgga ttgttgttgc ccggcacatg
     1921 cagggcccat atgtgcagcg ccaccacgcc cgcaatcgcg aacggcaaca gataatgcag
     1981 gctgaaaaag cgatgcagcg tcgcattgtc gacgccgaag ccaccccaca gccaggtcac
     2041 gatgccggtg ccaaggccgg gaatcagcgc atcgaaagcg ccgaagaaat tggtgatcac
     2101 ggtggcgccc cagaacgaca tctggcccca ggggagcgtg tagccgagaa aggccgtcgc
     2161 catcatcagg atgtagagga cgacgccaag aatccagatg atctcgcgcg gggccttgta
     2221 cgagccgtaa tagaggcccc ggaagatatg gatatagacg gcgaggaaga aaaaggatgc
     2281 accgttggaa tgcagatagc gcagcagcca tcccgaattc acgtcgcgca tgatgtgctc
     2341 gaccgaatcg aaggccagcc gcgaatccgg cgtgtagtgc atcgcaagca cgatccccgt
     2401 gagaatctgc gtcaccagga aagcgctgag gattccaccg aaggtgtacc agatattcaa
     2461 attgcgcggc gtcggatagg acgccagatg ctcctgggag aagcggatga tcggcagccg
     2521 cacgtcgagc cagcgctcga aacccgtttt cggttcatag gtcgaaggac cggacatcgt
     2581 cgttctcctc aacccacttt gattcgcgta tcggacagga acgcaaaaac cggcacctcg
     2641 agattgcgcg gcgcggggcc cacgcgcaca cggccggcgg tgtcgtattg cgaaccgtgg
     2701 caatggcaaa gccacccgcc ataatcgccc tgcggcgatt gtggggtact agcttccggc
     2761 gtacagccga gatgggtaca cacgcccacg agcaccagcc attcgggctt cacggctccc
     2821 gtcgccggct tgatgcgatt ctcatccgtg gcgggcgcgc tatcgggaag gctgtcattg
     2881 cgggcgaccg gatcgatcaa gtcgctcatt ttgaccgcac gcgcggcggc gatctcctcc
     2941 ggggtacggc ggcgcacaaa cagcggatga ctgcgccagg taaagacgac ggactgtccg
     3001 gccatgatgg gcgccagatc gacctcgatg gaggcgagcg ccagcacatc ggcggatgga
     3061 ttcatctgat cgatcagagg gatcagcgtt gccgccgcac ccaccgcgcc cagagcggcg
     3121 gtcgctatgt aaagaaaatc gcggcgcgtc ggttcagccg tttgcgaatg atccaaaatt
     3181 ctagcccttt gaaaccggtc ttcgaacacg ggtgcgaagg ctgcactcct cacaaaccgt
     3241 tgcgtccaaa gtccgtgcgt gtggtcacac tgccttgaag atcaaggcca cgtttacgtg
     3301 gcggctacca tagagagagt taattttcga gggtttgcgg cacgatgccg cagaaaatca
     3361 ggccttagct ggacggtttc gattggcgcg gccattggac gtcagcaggc aaacgcgcgg
     3421 atatggcctt gttcggcata agcgagaagc cggtcatcgc gtgtcatcaa ggtataacca
     3481 tattcgcgcg cggtcgtcgc catgatccgg tcggcgggat cggacggtgg cgaatcgggg
     3541 agaaaactcg acgcgagcaa aagttccggc ggcatcgcgg ccagacgcac accgggcagg
     3601 tttatcaaat gccggaaata gtcgagcggg cttctggccg cacgcaatcg tccgcgtgaa
     3661 aacaagagcc ccaactccca ggccgtgatc ggcgacagat agaccggttc tccgcgccgg
     3721 taggattggg tcagcaaatc cgccgccgca ggcgcaagtt gatcctccgc cgcccaaatc
     3781 gcagcacatg tatcaagcag cagcggcgtg ttcattgggg attttcacgg ccgtatttcc
     3841 tatccgccag ttctgcccat tcgggcatgg caggctgagt cagatcatat ccgggctcca
     3901 cgatgagcca tcccttcaag gccccgatca gcggatgccg cgccgccgga ttcttcgcgg
     3961 aatcgtcttg gaatgcacgc atgggctcac ccaatccccc acgtgattcg gcagccggcc
     4021 cgccggaagg cggactggga aaatgcgcac gtttaaaaat gagcttcttg cccgccatgt
     4081 cgacctgttc ggtccggtag ccagcgctca accaagcctt tgcgtgaacg tggttcgcag
     4141 cctcattggc ccaccacggg cgatggcggt aggccgaagg cggcagcttg gacccgatga
     4201 cctgttcgat ttctccaaat gtcattggca gctccgaagc ggcttgcgta ttgaggaacc
     4261 catgtaggga attatatttg gacatgaact acttccattt ataaattatg tactatacat
     4321 ggactccata tagaaggtcg tcaagcccta tgcgcctttg cctgttcgaa cccgacatcc
     4381 cgcagaacgc cgcctcgctg atccggctgg gcgcctgcct cggcgtagcc gtggacatcg
     4441 tggagccttg cggattcctg ttcagcgaaa gcggctttcg ccgggctggg atggattatg
     4501 tggaacgcgc cgacctcgtg cgccaccgct cctgggagga gtttcgcgcg acggcccaag
     4561 ggcggctcat tctcttgacg accaaagcac ccctgcccta cacggccttc gccttccagg
     4621 acggcgatat tctgatcctc ggacgtgaga gcgcgggcgt gcccgatcat gtccacgacg
     4681 cggcggaggc gcggttgcgc attccgctgc gttcgggttt gcgctcgatc aatgtggcgc
     4741 aggccggcgc cttggtgttg ggtgaagcgc tgcgtcagac caatggcttt ccggcggaag
     4801 gacttcctgg atgaacgtgc ccaatgccga ggttccatcg atcgcggcgc gcaaggaagc
     4861 ggcggccagc tggttccgag cgctgaggga caaaatctgt gccgcgttcg aagccatcga
     4921 agacgattac acgggcgcac ccgcatccgc acccggacgt ttcgtgcgca aggattggaa
     4981 gcgcccgaca gcggatggct cggacggcgg cggcggcacc atggccctga tgaagggccg
     5041 ggtgttcgaa aaagtcggag tcaatatttc caccgtgcac ggacggttcg agccgcaatt
     5101 cgccaaggaa attccaggcg ccgacaaaga cccctcctat tgggcaagcg ggatttcgct
     5161 agtggcccac atgcactcgc cccgggttcc caccgcccat ttcaacaccc gccatatcgt
     5221 cacgggcaaa tcctggttcg gcggcggcgg cgatctgacg ccgatgctca tggagcagcg
     5281 caacgccgaa catcgcaata cggtcgcctt ccacgccgcg ttccggcgcg cttgcgatgc
     5341 gcatggcccc gactattacg cgcgcttctc gaaatggtgc gacgaatatt tcacgattaa
     5401 gcaccgcggc gagacccgcg gcgtgggcgg catcttctac gactatctgg aaagtgattg
     5461 ggagaaggac ttcgccttca cgcaggatgt gggccgtgcc ttcctcgcgg cctatccggc
     5521 catcatccgc gactcgatga acgaggcctg gacgagagcg gaaaaggacg agcaactggt
     5581 acgcaggggt cgctatgccg agttcaatct ggtctatgat cgcggcacca gatttggact
     5641 gatgaccggg ggcaacgccg aagcgatcct gatgtcgctg cccccggaag tacgctggcc
     5701 gtaaggaagt acgctggcgt aagtttggcg gataacgaga cggggataaa accgggtgga
     5761 tctgttttcg tcttcggcgc tcttcgcgct ggcccaggtg ctgatgatca atatcgtgct
     5821 gtcgggcgac aatgctgtcg tcatcggcat ggtggccgca cgcgtcgaac ccgggaaccg
     5881 gcgcaacgtt atcctctggg gcatggtggc agccgtcgtc ttgcgtgtgc tgctggcgct
     5941 cgtcgcggtc gaccttctca acatcctcgg gctgttgctc gcgggcggga tcgtccttct
     6001 ctgggtggcc tggcggctct atcgcgacat ccgtgagcag cactcggaaa aagccgctct
     6061 taaggccaaa gagtcgcgcg gcgaatctag ccaaggcgct gtttctgcct catcgatgtc
     6121 gctgcgcaac gcgatctggc aaatcgcagt cgccgatatt tccatgtcgg tggacaatgt
     6181 gctggccatt gccggcgccg ccaaacaaaa catccccgtg ctggtgatcg gtcttgccgt
     6241 ttccgtcgcg ctgatgggcg tggcggcgac ctatatcgcg cggctgctgc aacgctttcc
     6301 ggcgctctcc tatatcggcg tcgcgctgat cgtctatgtc ggggccgaga tgatctggga
     6361 gggcagcggc gacgtaccgg aaatcctgaa gtttttccgc tgaagccgag caattttcct
     6421 tatcctcaga ccttcgtgaa gccgtagagg ttttgcggat tctcgaccag gatgcgctgg
     6481 cgtgtgcgct cgtccggagc ccagaccgcg agcaaatcga acagcagcgc atcgtccggc
     6541 ttggccgggc cgggcgaggt tggatgcggc caatcgctgc cccagaccag ccgttctgga
     6601 gcggccttca cgaaatcctg cgcgattcta ctggcctcgg ggtatggcgg tccaactttg
     6661 ctgttgagat aggcgccgga gagtttgacc caggcgcggc cggaatcgag cagtccgcga
     6721 acaatcgcgt gcgagggatg cgtgacgccg gcgggcagtg gcggaatccc cagatgatcg
     6781 aacaccagcg gcgccggcag ccgcttgagc agatcggcca tcgccacgat ctgctcgcca
     6841 cccatgttca tctgcagatg ccagccgagc ggcgccacgc gtctggccat cggctcgacc
     6901 atgtcgggca tcaccaccgc ggttgacgga tcgccgaggc tgaaacgggt tccccggatg
     6961 cccgccgcat tcagccgctt caattcggcg tcggagacat cgggatgcag caccgcgacg
     7021 ccgcgcgcgt tcgcgcccaa ttgcgcgatg gcatcgacag tcgcatcgtt gcgggtcgca
     7081 taattgcgcg gcgtcacgat gacgacgcgt gtggtgccga tgcgcttctg cagcaggcga
     7141 tattgatcga cggcggcatt ctccggcgcc acgcgcgggc tcggcgccaa tgggaagtgc
     7201 gccggattgt agatgtgcat atggcagtcg gcggcgttcg cgggcgcctt gagcttcggc
     7261 gactcggttc ccgtggaatt cgacaccgcc acctgcgcgc gcccctcttc tgtctgcagg
     7321 attgcggccg cgccaaatgc ggcgagacca gcccttttca tgaatgtccg ccggccaaat
     7381 tcatccatct cccgccccat tctggaaaca ggaaaatcct atcacgaagg gagaccgcgt
     7441 gggttgaggc tttcccgtca ggccggcggt tttcgcacag cgacggcgag gcccggaacc
     7501 ggctcgctgg cctggcgccg cagcacgcag gtcatgaggt ccgcaacctc gagcccggca
     7561 tatcgggcgg agctgcgcaa ataggcttcg ccgtggcgga accgtttgtc ttcggtctgc
     7621 tcccagccat gaccctccat gcgctcgacc gcgaaaagat agagaccgcc gggaacaagc
     7681 cgctggtaga cgcccgacag ggtaggagca agatcgccga aatagatcat ggtgtcggcg
     7741 gcaaggacga gatcatagcg ccgctcgacg gccagcagca tcgtctcgat atcgcccagg
     7801 atgagattgt catagatgcc gcggcggctg gcctcatcga tcatgcgcgg tgacagatcg
     7861 ataccatcga gccgcccgcc tttggccaaa tccttgaacg cgtcgcccac cagccccgtg
     7921 cccgatccca gatccaggat gcgcaaagcg cccgcgccgc ttccaagcac gcggttcgcc
     7981 agcgtgcgca gatgcagatg tgcccggtag cccagcgttt cgagcatgat ctgatcgtaa
     8041 tccgaggaga actcgtcgaa gagaccacgc acatagcccg gaggacagcg cgtcagccgc
     8101 ttcaatttga gcatcgccag atcgcggttc tcggctgtcg cggccagtgc gggatcgatc
     8161 tccagagcct tgtcgtagct cgtggccgct tcctgatagc gtctcagtgc catgaggata
     8221 ttgccccgat tgttccagct gatggcatgg tccggccgca gccggacagc ggcgtcgcaa
     8281 gcggctatcg cctcatccag ccggaacaat tggagaagca tgaccgcacg attgctgagc
     8341 gcttcgatga aatcgggttg aatcgccaaa gcgcggtcga aactggcgag cgcatcgtcc
     8401 aggcgcgaca tcgagacgag gacgctgccc cgattgttcc agcccgcggc atcgtccggc
     8461 cacaacgcca gcatgcgatc cagcgtcgcc aaggactcgg caggccggct tgcctcggcc
     8521 agaatagcgg cccggctcga aagagcctcc gccgagccgg gctcgatcga aagcgcggta
     8581 tcgatacagg cgagcgcgtc ctgccatctc tgaagacgcc gcagcaccg
//
LOCUS       DASYEG010000077         3048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4373202, whole genome shotgun sequence.
ACCESSION   DASYEG010000077 DASYEG010000000
VERSION     DASYEG010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4373202"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1197
                     /gene="obgE"
                     /locus_tag="VGM72_02915"
     CDS             160..1197
                     /gene="obgE"
                     /locus_tag="VGM72_02915"
                     /inference="COORDINATES: protein motif:HMM:NF008956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY4264242.1"
                     /translation="MKFLDQAKVYIQSGKGGNGIIAFRREKFIEYGGPNGGDGGRGGD
                     VWAEAVENLNTLIDYRFQQHMVAKSGGHGMGKEMHGANGADAILKVPVGTQIYEEDNE
                     TLIADLDRPGSRVQLLRGGNGGFGNAHFKSSTNQAPRHANPGQPAEEKTIWLRLKLIA
                     DVGLIGLPNAGKSTFLARVSEAKPKIADYPFTTLNPQLGMVRVTGVDFVVADLPGLIE
                     GAADGAGLGTRFLGHAERTSVILHLIDGTQSEIARAYKTIRAELQSYGHGLGEKSELV
                     VLNKSDAIPKAALAKKRSSLEKTSGQRVFVMSSVSGEGVDDVLRALAKTVDSARAKER
                     RASEPKASWAP"
     gene            1194..2330
                     /gene="proB"
                     /locus_tag="VGM72_02920"
     CDS             1194..2330
                     /gene="proB"
                     /locus_tag="VGM72_02920"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEY4264243.1"
                     /translation="MTDYFAAAKRIVVKVGSALLVDSATGALKRDWLASLAADVAALK
                     QEKKDIIIVSSGAIALGRRALKLKAGPLRLEESQAAAATGQVRLAQAYVDAFHGYDIT
                     VAQILLTLEDTEERRRYLNARETLNTLLALNAVPMINENDTVATAEIRYGDNDRLGAR
                     VASMMQADMLVLLSDIDGLYSANPQRDPTATHIPEVGAITPEIERMAEGPLSSTSKGG
                     MESKIAAAKIATSAGAAVIIANGHVLNPLRAIADGARTTIFQPRVSPAQAKKRWIAGG
                     LSALGTIVIDSGAERALLSGKSLLPAGVKSVSGTFERGDAVVVKSEDGRELARGLVAY
                     NTEDAKALVGRRTVDIEAILGYRGRDEMIHRDDMAVTALGGATP"
     gene            2327..>3048
                     /locus_tag="VGM72_02925"
     CDS             2327..>3048
                     /locus_tag="VGM72_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY4264244.1"
                     /translation="MNVPAMIPDALEADMLAIGRAARDAAVHLRGAPPQQKIDALLAA
                     SDAIRKTQADILSANDEDMKAAAVRDLTPALMDRLKLDAKRVAAMAQGVAEVAALPDP
                     VGRELARWRRPNGLDIARVSTPIGVIGIIYESRPNVTADAGALCLKSGNVAILRGGSE
                     SLRSSRAIHAALVNGLAAAGLPAAAIQLVPTTDRAAVGHMLAGLNGTIDMIVPRGGKS
                     LVARVQAEARVPVLAHLEGICHT"
BASE COUNT          596 a          914 c         1015 g          523 t
ORIGIN      
        1 atggttccat gacaatcccg cctccgggcg cgtgttggag aagcttggct gcattccgga
       61 tggcggcgag gtgcgcgcct gcctctcgcg cggaggcaat gtgttctgtc ataaggtcgt
      121 gctgaagcgg gccgttttcg aacggcggaa agaattgcaa tgaagttcct cgatcaggcc
      181 aaggtgtaca tacagtccgg caagggcggg aatggcatca tcgcgttccg ccgtgagaaa
      241 ttcatcgaat atggcggacc caatggcggc gatggcggcc ggggcggcga tgtatgggcc
      301 gaagccgtcg agaatctgaa cacgctgatc gattaccggt ttcagcagca tatggtggcg
      361 aaatccggcg gccacggcat gggcaaggag atgcatggcg cgaacggcgc cgatgccatt
      421 ctcaaggtcc ctgttggaac gcagatctat gaggaagaca atgagacgct gatcgccgat
      481 ctggatcgcc ctggctcccg cgtgcagctt ttaaggggcg gcaacggcgg attcggcaat
      541 gcccatttca agagttcgac caatcaggcg ccgcgccatg ccaatcccgg tcagccggcc
      601 gaggagaaaa ccatctggct ccggttgaag ctgatcgccg atgtcgggct gatcgggctt
      661 cccaatgcgg gcaaatcgac gtttctcgcc cgggtgtcgg aggcaaaacc caagatcgcc
      721 gattacccgt ttaccacgct caacccgcag ctcggcatgg tgcgggtgac gggcgtggat
      781 ttcgtggtcg ccgacctccc tggactgatc gaaggggcgg ccgacggtgc cggcctcggc
      841 acgcgttttc tcgggcatgc cgagcgcacc agtgtgatcc tgcatctgat cgacggcacg
      901 caatcggaaa tcgcgcgcgc ctacaagacc atccgcgcgg agctgcaatc ctatggtcat
      961 gggctcggtg aaaaatccga gcttgtggtg ctcaacaaga gcgatgccat acccaaggcc
     1021 gcactcgcca aaaagcgttc cagcctggaa aagacctccg ggcaacgggt tttcgtgatg
     1081 tccagcgtca gcggcgaagg cgtggacgat gtgctgcgcg cgctggcgaa gacggtggac
     1141 agcgcccgcg caaaggaacg gcgcgcatcc gaacccaaag cgagttgggc gccatgacgg
     1201 attatttcgc agccgcaaag cggatcgtgg tcaaggtcgg atcggcgctg ttggtcgact
     1261 ccgccacggg cgcgttgaag cgcgattggc tcgcgagttt ggccgcggat gtcgcggcgc
     1321 tgaagcagga aaaaaaggac atcatcatcg tctcctccgg cgccatcgcg ctcgggcgcc
     1381 gcgcgctgaa gctgaaggcg ggacccttgc gtctggagga aagccaagcc gcggcagcga
     1441 cggggcaagt gcgtctggcc caagcctatg tcgatgcctt ccatgggtat gacatcactg
     1501 tcgcgcaaat cctgctcacg ctcgaagaca ccgaggagcg ccgccgttat ctgaacgcgc
     1561 gcgagacctt gaacacattg ctggcgctga acgcggtgcc gatgatcaac gaaaacgaca
     1621 ccgtcgccac cgccgagatt cgctacggcg acaatgaccg gctgggcgcg cgcgtcgcaa
     1681 gcatgatgca agccgatatg ctggttctgc tttccgacat tgacgggctt tacagcgcca
     1741 atccgcagcg cgatcccacc gccacgcata ttcccgaagt cggcgcgatc acgcccgaga
     1801 tcgaacgcat ggccgaagga ccgctgtcga gcaccagcaa gggtggcatg gaatcgaaga
     1861 tcgcggccgc gaagatcgcc acttcggcgg gtgcggccgt catcatcgca aacggccacg
     1921 tccttaatcc cctgcgcgcg attgccgatg gcgcccgcac gacaatattc cagccgcgtg
     1981 tcagtcccgc gcaggccaag aagcgctgga tcgcgggcgg gctctcggca cttgggacga
     2041 ttgtcataga ttccggtgcc gagcgcgcgc tgctctcggg caaaagcctg cttccggcgg
     2101 gcgtcaaatc ggtaagcgga acgttcgagc gcggcgatgc ggtggtggtc aaaagcgaag
     2161 atgggcgcga gctcgcgcgc gggcttgtgg cctacaacac cgaggatgcc aaagcattgg
     2221 tggggcggcg caccgtcgat atcgaagcca ttctcggcta tcgtggccgt gacgaaatga
     2281 tccatcgtga cgatatggcg gtcacggcat tgggtggagc gacgccatga atgtgccggc
     2341 catgataccg gacgccctcg aagcggacat gctcgcaatc ggccgggccg cacgcgatgc
     2401 ggccgtacat ttgcgtgggg caccaccgca gcaaaagatc gatgcgctgc tggcggcgtc
     2461 ggatgcgatc cgaaagacgc aagccgacat tctcagcgcc aatgacgagg acatgaaggc
     2521 ggcggcggtc cgcgatctca cgcctgcgct gatggaccgc ctcaagctgg acgcaaagcg
     2581 cgtcgctgca atggcacagg gtgtggccga agtcgccgcc ttgcccgatc cggtgggccg
     2641 ggaattggcg cgctggagac ggcccaatgg tctggacatc gcgcgtgtgt cgacacccat
     2701 cggcgtgatc gggattatct atgagagccg gcccaatgtt acggcggatg ccggtgccct
     2761 gtgtctgaag tcgggcaacg tcgcgatcct gcgcggcggc tcggaatcct tgagatcgtc
     2821 gcgcgccatt cacgccgctt tggtgaacgg acttgccgcg gcgggtttgc ctgcggctgc
     2881 gattcagctc gtgccgacca ccgatcgcgc ggcggtaggc cacatgcttg ccgggctgaa
     2941 cgggaccatc gacatgatcg tgccgcgtgg cggcaagagc ctggttgccc gcgtgcaggc
     3001 cgaagcgcgc gtgccggtgc tggcccacct tgaagggatc tgccacac
//
LOCUS       DASYEG010000078         3775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3792456, whole genome shotgun sequence.
ACCESSION   DASYEG010000078 DASYEG010000000
VERSION     DASYEG010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3792456"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..2175)
                     /gene="recG"
                     /locus_tag="VGM72_02930"
     CDS             complement(94..2175)
                     /gene="recG"
                     /locus_tag="VGM72_02930"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008164.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY4264245.1"
                     /translation="MRPSILFPLFAEIRTLPGVGPKLERLIAKVAGPRLVNLIFDLPT
                     GVVDRSYRPKLIEAEPGRIATVDIHVLDHMPPRVKSQPYKIRVSDDTAMMDLVFFRAD
                     AKYLTALLPPGTRRLISGRIERFKDRLQMAHPDYVVAPEAATSLPQHEAIYGLTEGLA
                     AKTMAKAVRGALDRVPGLPEWQDPAFLRQRGFDAFHPALMAAHQPAHESDLLPGTMAR
                     QRLAYDELLANQLALLLTRARLRAPAGRRIAGDGQRKARAIAALPFALTDGQLSALAE
                     IEADMASDKRMLRLLQGDVGSGKTIVALLALLSAVEAGFQGALMAPTELLVRQHLASL
                     TPYAEAAGLRLAALTGRERGPQRTETLAKLAAGEIDILIGTHALFSDDVAFRDLGLAV
                     VDEQHRFGVHQRMQLQSKGRPADVLVMTATPIPRTLALTAYGDMDVSRITGRPPGRQP
                     VETRVMSAARLDDLIAHLRHALERGERAYWVCPLVEESEKIDLAAAQERAVMLRQILD
                     VPLGLVHGQMKAAERDAAMAAFKAGETRLLVATTVIEVGVDVPEATIMVVEHAERFGL
                     AQLHQLRGRVGRGTGKSSCLLVYHGALGQTAKARLATMRETDDGFVIAEQDLKLRGPG
                     EMLGRRQSGLEEFHLADPIAHADLLAVAHDDAKLILARDPDLKSPRGESLRVLLYLFG
                     RDEAVRYLRTG"
     gene            2320..2931
                     /locus_tag="VGM72_02935"
     CDS             2320..2931
                     /locus_tag="VGM72_02935"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HEY4264246.1"
                     /translation="MTQVFLEEARDYLIQSQKVLSAAAESQEFLGRIEAISEAIINCF
                     KSGGKVLLAGNGGSAGDAQHIAGEFLSRLNFDRNPLAAIALTVDTSVLTAIGNDYGYE
                     QVFERQVHGLGKPGDVFIGISTSGRSPNILRALRKARELGLVTVGFCGARKAEMLDLC
                     DIALCAPSESTPFIQQVHITSAHIICGEVEKQLFRQNASVAAI"
     gene            3114..3617
                     /locus_tag="VGM72_02940"
     CDS             3114..3617
                     /locus_tag="VGM72_02940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY4264247.1"
                     /translation="MFFDRDGVLNIDDGYTHKPSDLLWNLGAIAAVRAVNQAGWFAFV
                     VTNQAGIGHGYHSEADVITFHAHMSQLLAAEGAHIDEYLHCPFHPEAKLEAYRRESEH
                     RKPAPGMILELMSRWPVDKAKSFLVGDRESDLEAAARASIEAYHYRGGDLHELVLKGL
                     RSRGAAV"
BASE COUNT          728 a         1120 c         1184 g          743 t
ORIGIN      
        1 tggtttcgtg tccggcctgc gccaatgcgc gcgccacagc caggccgaca acaccggcgc
       61 cgatcacgac tgcgtccgcg gagaaaatct cgctcatccg gtcctgagat agcgcacggc
      121 ttcgtcgcgg ccaaacagat agagcaggac gcgcaaactc tctccgcgcg gacttttgag
      181 gtcgggatcg cgcgccagga tcaacttggc atcgtcatgt gcgacggcca gcagatcggc
      241 atgtgcgatt gggtcggcca gatggaattc ctccaatccg ctttgccggc gccccagcat
      301 ttcacccgga ccgcgcagtt tcagatcctg ttcggcgatg acaaagccgt catcggtttc
      361 tcgcatggtc gccagccgtg ccttggccgt ctggcccagc gcaccatgat agaccaatag
      421 gcaggaagat tttccggttc cgcgtcccac ccggccgcgc aattgatgca gctgcgccaa
      481 gccgaaacgc tcggcgtgtt ccaccaccat gatggtggct tccggcacat cgacgcccac
      541 ttcgatcacc gtggtggcga ccagaagccg ggtttcgcca gctttgaacg ccgccattgc
      601 ggcatcgcgc tcggcggctt tcatctggcc atgcacgagg cccaacggca cgtccaatat
      661 ctggcgcagc atcacggccc gctcctgcgc ggcggcgaga tcgatctttt cggattcctc
      721 caccaggggg cagacccaat aagcgcgctc gccccgctcc agggcatggc gcagatgcgc
      781 gatgagatcg tccaaccgcg ccgcgctcat cacgcgagtc tcgaccggct ggcggcctgg
      841 cgggcgaccg gtgatgcgcg agacatccat atcgccataa gcggtaagcg ccagggtgcg
      901 cggaatgggc gtcgccgtca tcaccagtac gtcggcgggg cggcccttgc tctgcaactg
      961 catgcgctga tgcacgccga agcgatgttg ctcgtccacc accgccagtc ccagatcgcg
     1021 gaaggcgacg tcatcggaaa acagcgcatg cgtgccgatc agaatatcga tctcaccggc
     1081 cgccagcttg gccaatgttt cggtgcgctg tgggccgcgt tcgcgccccg tgagtgccgc
     1141 cagccggaga ccagccgcct cggcataagg cgtcagcgat gccaaatgct gacggaccag
     1201 caattccgtc ggtgccatca acgcgccctg gaaaccggct tcgaccgcgc tcagcaaggc
     1261 caacagcgcc acgatggtct tgcccgagcc gacatcgccc tgcagcagcc gcaacatgcg
     1321 cttgtcgctg gccatgtcgg cttcgatctc ggcgagggcg gagagctgtc catcggtcaa
     1381 ggcgaagggc agggctgcga tggcgcgggc cttgcgctgg ccgtcgcccg caatgcgtcg
     1441 cccggcaggt gcgcgcagcc gggcgcgggt cagcagcaac gccagctgat tggccagcag
     1501 ctcgtcatag gcaaggcgct ggcgcgccat cgtgccggga agcaggtcgg attcgtgtgc
     1561 gggctgatgc gccgccatca acgcagggtg aaacgcatcg aagccgcgct ggcgcaggaa
     1621 tgcggggtcc tgccattccg gcaggccggg cacgcggtcc agcgccccgc gcactgcctt
     1681 ggccatggtc ttggccgcga ggccctcggt caggccatag attgcttcgt gctgaggcag
     1741 ggacgtggcc gcttccgggg ctaccacata atcggggtgc gccatctgca gtcggtcttt
     1801 gaaacgctcg atacggccgg aaatcaaccg ccgcgtgcct ggcggcagga gagcggtgag
     1861 gtatttggcg tcggcgcgaa aaaacaccag gtccatcatt gcggtgtcgt cgctgacgcg
     1921 gattttatag ggctggcttt ttacccgggg cggcatgtgg tcaaggacat gaatatcgac
     1981 ggtcgcgatg cggccgggct cggcctcgat cagcttggga cgataggaac ggtcgaccac
     2041 gccggtgggc agatcgaaaa tcaggttcac cagcctggga ccggcgactt tggcgatcaa
     2101 tctttccagc ttcgggccca ccccgggcag ggtgcgaatc tcggcgaaaa gcgggaacag
     2161 aatcgaaggt cgcatcggtg caatttaagg ttcccgcccc gggttgcgcc actgggggcc
     2221 gcgacctagc gttggatata tggatacgcc aagctaatcg cgctactgtt cggcccatca
     2281 ggcttgtata gattcagcga gatttgacgt gggaccgtta tgacacaggt atttttagag
     2341 gaagcgcggg actatctgat tcagtctcag aaagttttgt ccgcggccgc cgagagccag
     2401 gaatttcttg gtcgaattga agctattagc gaagccatta tcaactgctt caagtccggc
     2461 ggtaaggtat tgttggccgg caacggtggc agtgcgggag atgcgcaaca tatcgcgggg
     2521 gaatttctct cccgattgaa ttttgatcgc aatccattgg cagccatcgc actcaccgtg
     2581 gatacttcgg tgctgactgc tattggcaat gattatggct atgagcaagt tttcgaacgc
     2641 caagttcatg ggctgggtaa accaggggac gtatttatag gtatttcaac ttcaggacgg
     2701 tctcccaata ttcttcgagc gttgcgcaaa gcgagggagc ttggcttggt tactgtcgga
     2761 ttttgtggtg cgcgtaaggc agaaatgctg gatctatgtg atatcgcgct ttgtgcacct
     2821 tctgaatcca cgccgtttat ccagcaggta catattactt cagctcacat catttgcggc
     2881 gaagtggaaa aacaattatt tcggcagaat gcgagtgtcg cagcgatctg agtgattagc
     2941 tcggatacgt cttagagcgc ctcctctcca acctgtgctg tattgcgcgg caaggcggcg
     3001 cgagctcatc gttgggaaac caaactcagg gagcgatgat tttgcaacac ggagttaccg
     3061 agaatcgcga acgctcggag gcggttttgt gccataacct ctgccgtccc gctgtgttct
     3121 tcgaccgcga tggcgtgctt aacattgatg acggttatac gcataagccc agtgatctgc
     3181 tctggaacct tggcgccata gccgcggtcc gggcggtcaa tcaagcgggc tggttcgctt
     3241 ttgtggtgac caatcaggcg ggtatcgggc acggctatca cagcgaggcg gatgtgatca
     3301 cctttcacgc tcatatgagc cagctcctgg ccgcggaagg cgcgcatatc gacgaatacc
     3361 tgcattgccc ctttcacccc gaggccaagc tcgaggccta tcgccgcgag agcgagcatc
     3421 gcaaacccgc gccgggaatg attctggaac tgatgtcccg ctggccggtg gacaaagcga
     3481 aaagctttct ggtcggcgat cgggaaagcg atctggaagc cgcggcgcgt gcctcgatcg
     3541 aggcctacca ttatcgcggc ggggatctac acgaactggt cctcaagggg ctgcgctcgc
     3601 gcggcgccgc cgtctaaagc ctgttgtccc ggaagagggg cagggctata tccccgcgca
     3661 tggatgacag cgaaaaaggt ggctcttcgg agaaccgccg caaaaggctt ttgttccgtg
     3721 cccagcgccg gggcttcaag gaagtcgatc tgatcttcgg cgcttatgcg gaggc
//
LOCUS       DASYEG010000079         5082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2341137, whole genome shotgun sequence.
ACCESSION   DASYEG010000079 DASYEG010000000
VERSION     DASYEG010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2341137"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VGM72_02945"
     CDS             <1..199
                     /locus_tag="VGM72_02945"
                     /inference="COORDINATES: protein motif:HMM:NF016195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HEY4264248.1"
                     /translation="QYYAYIEILDEREMEVFSSEEAGAELWRAYRMVAEAWKNFATKR
                     IAKPADIFPVFHELFRKREAV"
     gene            480..1973
                     /locus_tag="VGM72_02950"
     CDS             480..1973
                     /locus_tag="VGM72_02950"
                     /inference="COORDINATES: protein motif:HMM:NF008737.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEY4264249.1"
                     /translation="MTTPLFTESEWTFEALDRVLKAVEEIALDDLKLDVYPNQIEIIS
                     TEQMLDAYSAMGMPLMYHHWSFGKLFAREESLYRAGHIGLAYEMVINSNPCISYLMEE
                     NSMTMQTIVIAHAAFGHNHFFKNNYLFQQWTNAESILEYLAFAKRYIVACEERHGHAE
                     VEAVLDSAHALMDQGVFRYRRPPRPSRARILEKQRRRAEHAEEDYSELWRTLPAAAEP
                     APPERDVWDFDEDELGEGPKLPEENLLYFLEKYSPVLKSWQREILRIVRHLAQYFYPQ
                     RQTKVMNEGAASFVHHTIMHRLYDKGLLTEGAIFEFLASHTAVVFQPEFEDPRFSGIN
                     PYALGFGMMTDIKRISEEPTDEDRAWFPAFAGQGDWRGVLKDAWANYRDESFIEQFLS
                     PKLMRDFRLFALADEAEASAVTVSAIHDESGYRRVRSTLARQYDIGESDPNIQVTGAN
                     LRGNRKLFLEHRMHRDVPLHAGLKAQVMPHIERLWGHEVVLEDITNN"
     gene            complement(1990..2063)
                     /locus_tag="VGM72_02955"
     tRNA            complement(1990..2063)
                     /locus_tag="VGM72_02955"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2029..2031),aa:Gly,seq:ccc)
     gene            2159..3199
                     /locus_tag="VGM72_02960"
     CDS             2159..3199
                     /locus_tag="VGM72_02960"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEY4264250.1"
                     /translation="MADQPILQTTIAGSLPKPSWLAEPEKLWAPWRLEGAALAAGKRD
                     ATLIWLKEQEDAGIDIVTDGEQSRIHFVHGFLERIEGIDWGRKTRMGIRNNRYEVEVP
                     TVTGALRRRGPVHSDDMRFARAHTRRKLKVELPGPMTICDTIADAHYGRRADMAMAFA
                     KLLNEEARELAQLGVDVVQLDEPAFNVFMDEVKEWGVAALDEAIKGLTCKTAVHICYG
                     YGIEANINWKNTLGSEWRQYEEIFPAINASRIHQVSLECAASKVPMSLIALLKDKEIM
                     VGAIDVATNAVETPEAVASVLRSAMKYAAPEQIYPCTNCGLAPLPREVAAGKLKSLAA
                     GAALLRKELDRA"
     gene            complement(3207..4538)
                     /locus_tag="VGM72_02965"
     CDS             complement(3207..4538)
                     /locus_tag="VGM72_02965"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY4264251.1"
                     /translation="MKRQLIFAGLACLTIAPVAIAAELQLTPAPGFPTQNEPTGTPPG
                     VVALQNRNSTGFPAPMLKEGEPIETRPPEKEDDHSVFAGQTRAPYHASVPYTVTTLSD
                     TLKAPWSFAFLPGGEILITEKGGTMRIRNAQGALSEPLTGVPTVLAVGQVGLLDVALD
                     PQFANNHRLYLSYSEPVGETYSNIAVARARLDGHALSDVKVIFRAKPGLPARLLSSNQ
                     GGRIAIGRDGNLFVIIGDRSKSPPWDMAQRLDTDLGKMIHITPDGDPAPGNPFIGKAG
                     ALPEIWSIGHRSEEGLTFDPTTGKLWEVEDGPRGGDELNLMEPGKNYGWPVALHGIDY
                     PGQTIGEGIVEKAGIEQPRYYWDPVIAPSGLAFYTGNLFPQWKGSVFIGALRGQMLDR
                     LTMNADKVVAEEPLLVDLHSRIRDVRIGPEGAVYVLTDGPNNAKLLKLTPK"
     gene            4754..>5082
                     /locus_tag="VGM72_02970"
     CDS             4754..>5082
                     /locus_tag="VGM72_02970"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4264252.1"
                     /translation="MRGFALFIAGALVGGLAVQAAIAQNQGPNHGIVGLNHVGINVPD
                     LDKAVTYYTKTMGFPEAFRISNAQGQVQLVYVQISKNTFIELQPANGRPIGVSHFGLH
                     VENMKDAV"
BASE COUNT         1032 a         1493 c         1574 g          983 t
ORIGIN      
        1 tcaatattac gcctatatcg aaatcctgga cgagcgcgag atggaagtgt tctccagcga
       61 agaggcgggc gcggaattgt ggcgcgccta tcgcatggtc gccgaagcgt ggaagaattt
      121 cgccaccaag cgcatcgcca aaccggccga tatcttcccg gtgttccacg agttgttccg
      181 caagcgggag gcggtgtgat gccaaagagg cgcggcccaa agaggcgcgg catggtcgcg
      241 aggccatcgc caccccatat gcgagggatg tgtcttggag cctccctgcc gcgcttgtgc
      301 ccagtgcacc gaaattgcac cgaaattcgc accaaatttt atggcgcatt aaccgtgttt
      361 tcggcgggaa agagcgcagt ttttcatgac ctcgatggaa aaataggggg gagggggggg
      421 gacgcctatt tccgcctgga aatggcgacc gctggtgaaa gagacggtcg ggcaacagca
      481 tgacgacgcc gcttttcacc gaatcggaat ggacgttcga agcgctcgac cgcgtgctca
      541 aggcggtgga agagatcgcg ctcgacgatc tgaagctcga tgtctatccc aaccagatcg
      601 agatcatctc caccgaacag atgctggacg cctattccgc catgggcatg ccgctgatgt
      661 atcaccattg gtccttcggc aaattgttcg cgcgcgagga gagtctctac cgcgcaggcc
      721 atatcgggct cgcctatgag atggtgatca attccaatcc ctgcatctcc tatctgatgg
      781 aggagaactc gatgacgatg cagactatcg tcatcgcgca tgcggctttc ggccacaatc
      841 atttcttcaa gaacaattat ctcttccagc aatggaccaa tgccgaaagc atcctggaat
      901 atctcgcctt cgccaaacgc tacatcgttg cctgcgaaga acgccacggc catgccgagg
      961 tcgaagcggt gctcgattcg gcccatgctc tgatggacca gggcgtgttc cggtatcgcc
     1021 ggccgccgcg tccatcgcgc gcgcgcatat tggaaaagca gcgccgccgc gcggaacacg
     1081 ccgaggagga ctacagcgag ctgtggcgca cgttgccggc cgccgccgaa ccggcgccgc
     1141 ccgaacgcga tgtctgggat ttcgacgagg acgaattggg cgaaggacca aagttgccgg
     1201 aagagaattt gctgtatttc ctggagaaat attcgccggt gctcaaatcc tggcagcggg
     1261 agatcctgcg catcgtgcgc catctggcgc agtattttta tccccagcgc cagactaagg
     1321 tgatgaacga gggcgcggca tccttcgtgc accacaccat catgcacaga ctctacgaca
     1381 aggggttgtt gacggagggc gcgatattcg aattccttgc aagccacacc gccgtcgtat
     1441 tccagcccga attcgaagac ccgcgctttt cggggatcaa tccctacgcg ctcggtttcg
     1501 gcatgatgac ggatatcaag cgcatttcgg aagaaccgac ggacgaagac cgcgcctggt
     1561 tccccgcctt cgccggccag ggcgattggc gcggggttct gaaggacgcc tgggcgaatt
     1621 accgcgatga aagcttcatc gagcagttcc tgtcacccaa gctgatgcgc gatttcaggc
     1681 ttttcgcatt ggccgacgag gccgaagctt cggccgtcac cgtgtcggcc attcacgacg
     1741 aaagcggtta ccggcgcgtg cgctcgaccc ttgcccgcca gtacgatatc ggcgaatccg
     1801 atcccaacat ccaagtcacc ggcgccaatc tcaggggcaa tcggaagctg ttcctcgagc
     1861 accgcatgca tcgcgacgtg ccgcttcatg ccgggctgaa ggcccaggtg atgccgcata
     1921 tcgaacggct atggggccat gaggtggtgc tggaggatat cacgaacaac tagctgagcg
     1981 gccacgggtt ggagcgggcg aagggaatcg aaccctcgta tgcagcttgg gaagctgccg
     2041 ttctaccatt gaactacgcc cgcgaaccga agacttaggc tattgtccgt gccgcggttt
     2101 atcccccgcg ccggatagaa tatcaatccg cctgccgcct cgccatagga gccatttcat
     2161 ggccgaccag ccaatattgc agacgaccat cgccggcagc ctgccgaaac cgtcctggct
     2221 tgcagagccg gaaaaattgt gggcgccgtg gcgtctggaa ggcgcggcac tcgcggccgg
     2281 taaacgcgac gccacgctga tctggctcaa ggagcaggag gatgccggta tcgacatcgt
     2341 caccgatggc gagcaatccc gcattcattt tgtccatggc tttctggaac ggatcgaggg
     2401 gatcgattgg ggcaggaaga ccaggatggg aatccgcaac aatcgctatg aagtggaggt
     2461 gccgacggtg accggcgcgt tgaggcggcg cggaccggtg cacagcgatg acatgcgatt
     2521 tgcgcgcgcc cacacgcgtc gcaagctcaa agtcgagttg cccgggccga tgacgatttg
     2581 cgataccatc gcggatgcgc attacggacg ccgggccgac atggcgatgg cgtttgccaa
     2641 gctcctcaac gaagaggcgc gggagctcgc gcaattgggc gtcgatgtcg tccagctcga
     2701 tgagccggcg ttcaacgtct tcatggacga ggtgaaggag tggggcgtcg cggcgctcga
     2761 tgaggccatc aagggcctca cctgcaagac cgcggttcac atctgctacg gctatgggat
     2821 cgaggccaac atcaattgga agaacacgct gggctcggag tggcgccaat atgaggaaat
     2881 attcccggca atcaatgcga gccgcatcca tcaagtctcg ctcgaatgcg ccgcctccaa
     2941 agtcccgatg tcgctcatcg cgttgttgaa ggacaaggag atcatggtcg gcgccatcga
     3001 cgtggcgacc aacgctgtgg agacacccga ggcggtggcc tccgtcctga ggtcggcgat
     3061 gaaatatgcg gcgcctgagc aaatctaccc ctgcaccaat tgcgggctcg caccgctgcc
     3121 gcgcgaagtc gcggcaggca agctcaaatc gctcgccgcc ggcgcagcac tcttgcgcaa
     3181 agaactggat cgcgcctgag cgtgcgctat ttcggcgtca gtttgagcag tttggcgttg
     3241 ttggggccgt cggtcaggac atagacagcg ccctccggtc caatccgcac atcgcggatt
     3301 cgcgaatgca ggtccaccag caacggttct tccgcgacca ccttgtctgc gttcatggtg
     3361 agccgatcga gcatctggcc gcgaagcgca ccgatgaaga cgctgccctt ccattgcggg
     3421 aatagattgc ccgtgtagaa ggcaaggccg gagggggcga tgacggggtc ccaataatag
     3481 cgtggctgct cgatgcccgc tttctcgacg atgccctcgc caatggtttg gccgggataa
     3541 tcgatgccat gcaaggcgac cggccaacca taattcttgc ccggctccat gaggttgagt
     3601 tcgtcgccgc cgcgcgggcc gtcctccact tcccataatt tgcccgtcgt gggatcgaac
     3661 gtcaggcctt cctcgctgcg atggccgatg gaccagattt caggcagcgc gcccgccttg
     3721 ccgatgaacg gattgccggg cgcgggatcg ccgtccgggg tgatatggat catcttgccg
     3781 agatcggtat ccagcctctg cgccatgtcc cacggcgggg atttggagcg atcgccgatg
     3841 atgacaaaca gattcccgtc gcgtccgata gcgatgcgcc cgccctggtt tgacgaaagc
     3901 agtcttgcgg gcaagcccgg cttggcgcgg aagatcacct tcacatcgct cagcgcatgg
     3961 ccgtccaaac gcgcgcgcgc aacggcgata ttgctgtagg tctcaccgac cggttcgcta
     4021 taggacaaat agaggcgatg gttgttcgcg aattgtggat cgagcgccac atccagaagc
     4081 ccgacctgtc cgactgcgag caccgtcgga acgccggtaa gcggctcgga gagcgcgccc
     4141 tgcgcattgc ggatgcgcat cgtgccgcct ttttcggtga tcaggatttc gccgccgggc
     4201 aggaaggcga agctccaggg tgcctttagc gtatcgctga gggtggtcac cgtgtagggc
     4261 acgctcgcgt gatagggcgc acgcgtttgg ccggcaaaga ccgagtggtc gtcttccttt
     4321 tccggcggtc gggtctcgat cggctcgcct tccttcaaca tcggtgcggg gaaacccgtc
     4381 gaattgcggt tctgcagagc gactacgccg ggcggtgtgc cggtgggttc gttctgcgtg
     4441 ggaaaacccg gggccggcgt cagctgcagt tcagcggcaa tggcgacggg agctatcgtc
     4501 aggcaagcca gccccgcaaa gatcaattgc cgtttcatgc tccgtgctcc cgttctattt
     4561 cccggccgaa aataaccggt ttctttcggg aagtcgaagg ggccagccga cgcgcgagcg
     4621 ggccggactt ttgcctacga agctccttct aaattcgggt tcggcatttc ggttcggcac
     4681 gggaacccgg gcttatcgat gccaggcctt cggtgctatc gtccgggtca cgcaaaaaaa
     4741 tggggagttc gtcatgcgcg gatttgctct attcatcgcc ggggctttgg tgggcggact
     4801 ggcggttcag gcggccatcg cccagaacca agggccaaat cacgggatcg tcggactcaa
     4861 tcatgtcggg atcaatgttc ccgacctcga taaggccgtg acgtattaca ccaagacgat
     4921 gggcttccct gaggcattcc ggatcagcaa tgcgcaggga caggtgcaac tcgtctatgt
     4981 ccagatcagc aagaacacat tcatcgaatt gcaacccgcc aatggtcggc ccatcggcgt
     5041 cagccatttt gggctgcatg tcgagaacat gaaagatgcg gt
//
LOCUS       DASYEG010000080         7223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4373567, whole genome shotgun sequence.
ACCESSION   DASYEG010000080 DASYEG010000000
VERSION     DASYEG010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 7223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7223
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4373567"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VGM72_02975"
     CDS             <1..422
                     /locus_tag="VGM72_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HEY4264253.1"
                     /translation="LAAVASKLAAEIHHLAILKSDVEDADHNVTRFLIMSPQAEDAQN
                     DGTPAITSFIFRVRNVPAALYKALGGFATNGVNLTKLESYQLEGSFNATQFFADIDGH
                     PEERNVQLALEELSFFTSSLKILGVYPASPFRLTLKS"
     gene            complement(426..1340)
                     /gene="nudC"
                     /locus_tag="VGM72_02980"
     CDS             complement(426..1340)
                     /gene="nudC"
                     /locus_tag="VGM72_02980"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEY4264254.1"
                     /translation="MIAFAGNSLDRASEKRSDAAWLAAKRRHANARVLPLWKLQPLLF
                     EPEKASEGTGLGFLEGVLASGLGAPDAVEVFLGLDGHTPYFARDISALTDPLAAALAH
                     HGHFRDARSAAGLIAAGDIAILGQAKALIDWHGRHGFCANCGTRTSLADGGYRRLCPV
                     CKAEHFPRTDPAVIMLVTAGDHCLLGRNKRFAGGHYSTLAGFIEPGETIEEAVRREVY
                     EEVRVRVDAVRYFASQPWPFPSNLMIGCFAHSEQADIQVDGNEIVAARWFDRANVKRL
                     ISGASDEVGLPRSDAIAFHLIRTWAEEG"
     gene            1572..2426
                     /locus_tag="VGM72_02985"
     CDS             1572..2426
                     /locus_tag="VGM72_02985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY4264255.1"
                     /translation="MIRSRARRAGEGARLRAYIHELETTKAQLEATSSNLAAALVSAD
                     AANKAKSEFLAAMSHELRTPLNAVIGFSEVMLNEVFGPLGHGRYREYIENIRGSGTHL
                     LGLINDVLDMSRLDAGELTLSREELKFADLIGEAVRAITLQAEAAGIALIEDIEPWLP
                     PIQADRRRMRQVLINLLTNAVKFTHAEGTVRISAFRHGDNLAIQVCDTGIGMADDEIP
                     RALERFGQVDSRLSRKYEGAGLGLPLAKQLTELHGGHLELQSVPGAGTQVTVILPLEM
                     ISSERQAA"
     gene            2473..2569
                     /gene="ffs"
                     /locus_tag="VGM72_02990"
     ncRNA           2473..2569
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VGM72_02990"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2599..4377
                     /locus_tag="VGM72_02995"
     CDS             2599..4377
                     /locus_tag="VGM72_02995"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF006585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY4264256.1"
                     /translation="MDESQEPRLGLDDGGVPAAHPPAGEPYRVLARKYRPQDFTGLIG
                     QDALVRTLKNAFASGRIAHAFMLTGVRGVGKTTTARIIARALNCVGPDGKRTTPTIDP
                     CGVCEPCRAIAESRFVDVQEMDAASRTGIDDIREIIEGTRYAPAAARYKVYIIDEVHM
                     LSKQAFNGLLKTLEEPPPHVKFIFATTEIRKVPVTVLSRCQRFDLRRIESAALSAHLA
                     HIAGEERIAIESAALSLIARAAEGSVRDALSLLDQAIAHDAGETDTIEAESVRAMLGL
                     ADRGRVLDLFEKLMGGEIPLALAEFKSLYDDGADPLVVIQDLLETTHFLTRVKVAPGA
                     EGFFDGGSGEAARAVTMAGKLNVPALTRAWQMLLKGLIEVRDASNPYPAAEMALVRLA
                     YAADLPPTERLVRSLRDSEPASVSPPPASPRGGSGGSPRAQSAVLASEEPKPLHDEIP
                     ASAASAPRMEIRTLEDIVALAKDRGARLLATQLENNVHLVSLERGRIQFRPSAQAPTA
                     LAGDIAQRLRDWTGERWIVTLASEGGAPTIAGQRIADERAKKDAVAQEPFVRAVLDAF
                     PGAEIVAVRDRETPEPASMASDEEQN"
     gene            4377..4697
                     /locus_tag="VGM72_03000"
     CDS             4377..4697
                     /locus_tag="VGM72_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEY4264257.1"
                     /translation="MDSNLLRQMQEMQRRADELQKGLAAMEVEGVSGGGLVRATVNGL
                     GELKALSIDSSLLKPEEKGIVEDLVVAACADGKMKLEARRMQDSQFLQDLLKSFSAGL
                     PDSD"
     gene            4701..5303
                     /gene="recR"
                     /locus_tag="VGM72_03005"
     CDS             4701..5303
                     /gene="recR"
                     /locus_tag="VGM72_03005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY4264258.1"
                     /translation="MAGSPAGHEIERLIQLLAKLPGLGPRSARRAALALLKKRETLLE
                     PLADAMAKAASALKQCSHCGNLDTTDPCALCRDPARETGIICVVEDVADLWALERASV
                     FRGRYHVLGGALSALDGVTPERLNLPSLLERVKAGAGEIILAMNATVEGQTTAHYLLD
                     TLEPLGVKITRLAHGVPVGGELDYLDEGTLSAAFKARRAF"
     gene            complement(5369..5953)
                     /locus_tag="VGM72_03010"
     CDS             complement(5369..5953)
                     /locus_tag="VGM72_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264259.1"
                     /translation="MMKDVRGDTAPTADATNNTTKIIVAAVVALGFGAMGAYSYETGT
                     WNSAPVAKHIVDSKSLPTTAVVPPTPPSETPQQSSADVPSATPPAAVEKPAAAPNKDN
                     HAQNVPPASITPPVRVARAVQAPAPVIPPAPESQPTVNESPAAPEAAAPASAPEPAAP
                     AAIPESAQDSAPVTAPMPEAPVAPEQPAETAPAQ"
     gene            6167..6913
                     /locus_tag="VGM72_03015"
     CDS             6167..6913
                     /locus_tag="VGM72_03015"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY4264260.1"
                     /translation="MKNCFLVAAIAGLLCAGAGNAAEIKVLSTQATEQSYRELVPQFE
                     KTTGHKVTTIFTGTLDADKRLAAGERYDLLIMSSPSIDEHIKDGKLAPGSRVDLAKSG
                     VAVAVLRGAPKPDIGSAEALKKTLLAAKTIGYSTGPSGNIVMALFQRLGIADQIKPKL
                     KQTATGVFVGSIIASGEVEVGFQQVSELSQFPGIDYVGPLPKEIQQYTIFSSGIVAGT
                     GQADAAKALVKFLTSPAAAPVFKKRGMEPA"
BASE COUNT         1378 a         2179 c         2310 g         1356 t
ORIGIN      
        1 cgctggcggc ggttgcctcc aaattggcgg cggaaatcca ccacctcgcg attctgaaga
       61 gcgatgtcga agacgccgac cacaatgtga cgcgttttct catcatgtcg ccgcaagccg
      121 aagatgcgca gaatgacggc acaccggcga tcaccagctt catcttccgg gtgcgcaatg
      181 tgccggcggc gctttacaag gcgcttggcg gatttgccac caacggggtc aatctgacca
      241 agctcgaatc ctatcagctc gaaggttcgt tcaacgcaac gcagttcttc gcggacatcg
      301 atggccatcc cgaggagcgc aatgtccagc ttgcgctaga agagttgagc ttcttcacct
      361 cgtccttgaa gatcctgggc gtctatcccg cgagtccctt ccgcctcacg ctgaagagct
      421 gacgatcatc cttcttccgc ccaggttctg atcaggtgga aggcgatggc gtcggaacgc
      481 ggcaaaccca cctcatcgct ggcgccgctg atgagacgtt tcacatttgc cctgtcgaac
      541 cagcgcgcgg ccacgatttc gttgccatcg acctggatat cggcctgctc gctgtgcgcg
      601 aaacagccga tcatcagatt ggagggaaag ggccagggct ggctggcgaa atagcgcacg
      661 gcatccacgc gcacgcgcac ctcctcatag acctcgcggc gcaccgcctc ctcgatggtc
      721 tcgccgggct cgatgaaacc ggcgagcgtc gaataatggc cgccggcaaa tcgcttgttc
      781 cgccccagaa ggcaatggtc gccggccgtg accaacatga tgacggcggg atcggtccgt
      841 ggaaaatgct cggccttgca gaccgggcaa agccggcggt agccgccatc ggccaggctc
      901 gtgcgggtgc cgcaattggc gcagaaacca tgccgcccgt gccaatcgat cagcgctttt
      961 gcctgaccga ggatcgcgat atccccagct gcgatgagac cggccgcaga gcgcgcatcg
     1021 cggaaatggc catgatgcgc gagcgcggcg gccaacggat cggtcagcgc cgagatatcg
     1081 cgggcgaaat agggtgtgtg gccatccaag cccaggaaaa cctcaaccgc gtccggcgcg
     1141 ccgagtcccg aggcaagaac gccttcgagg aagccgagcc cggttccttc cgatgccttc
     1201 tccggctcaa aaagcagagg ctgcagcttc caaaggggca ggacgcgcgc gttcgcatgc
     1261 cgccgcttgg cggccagcca cgccgcatcg ctccgctttt cgctcgcccg atccagcgaa
     1321 ttgccggcaa acgcaatcat tgcacccccg caagaagacg ccgaacctaa acgggacgac
     1381 gcctcaggca aaggcgcttc tgtgggcaaa tcgctgaaat gcacctgggg aattagccgt
     1441 gaattaaccg gattatgcga gcttttccca tgcttcgcgt cctttccgac agcgccggtc
     1501 gaacgggtcc tccgctcgtt tggctcatgg gattcgccct cgtctgcttc tgcggggcgg
     1561 cgttgcgtct gatgatccga agtcgcgccc gccgggccgg cgagggcgcg cggctgcgcg
     1621 cctatatcca tgaactcgaa accaccaagg cgcaattgga ggcgacatcc tcgaatctcg
     1681 ccgcggcgct ggtttcggcc gatgccgcga acaaggcgaa atcggaattc ctagccgcca
     1741 tgagccatga gctacgcacg cccttgaacg ccgtgatcgg cttttccgaa gtgatgctga
     1801 acgaggtgtt cggacctttg gggcatggac gctatcgcga atatatcgaa aacatccgtg
     1861 gcagcggcac gcatctcttg ggcctcatca acgatgtgct cgacatgtcg cggctcgatg
     1921 ccggagaact cacgctttcg cgcgaagaac tgaagttcgc cgatctgatt ggcgaggcgg
     1981 tgcgcgcgat cacgctgcag gccgaagcgg ccggtatcgc gctgatcgaa gacatcgaac
     2041 cctggctgcc ccctatccag gcggaccgcc gacgcatgcg ccaggtgctc atcaacctgc
     2101 tgaccaatgc ggtgaaattc acgcatgccg agggcaccgt ccggatctcg gcatttcgtc
     2161 acggcgacaa tctcgcgatt caggtctgcg ataccggtat cggaatggcc gacgacgaga
     2221 ttccgagggc gttggagcgt ttcggacagg tcgatagccg gctgtcacgc aaatatgagg
     2281 gcgccggcct tggtttgccg ctcgccaagc agctcacgga actgcatggc gggcatcttg
     2341 agttgcagag tgtgcccggc gcgggaacac aggtgacggt gatcttgcca ctcgaaatga
     2401 tttcctcgga acgtcaggcc gcctagtttc cgcccttgct tcacgggcgc gcgcccctga
     2461 tatagtgcgt tgggaggttg gcgacggacg agacactcgc caacccggtc aggtccggaa
     2521 ggaagcagcc gtaacgagcg ccgtttcggg tcgctcgcca gcctcctgct tattttttcc
     2581 gaaagcgcga accttacgat ggacgagtcc caggaaccgc gtttgggcct tgacgacggc
     2641 ggcgttccgg ctgcgcatcc gccggcgggc gaaccctatc gcgtgctcgc ccgcaagtac
     2701 cgcccgcagg atttcaccgg gctcatcggg caggacgcgt tggtccgtac cctgaagaat
     2761 gccttcgcgt cgggccggat tgcccatgcc ttcatgctga ccggggtaag aggcgtgggc
     2821 aagaccacga ccgcgcgcat catcgcgcgc gctttgaatt gcgtgggtcc tgacggcaag
     2881 cgcacaacgc cgaccatcga cccttgcggg gtgtgcgagc cttgccgggc cattgccgaa
     2941 tcgcgtttcg tcgatgtgca ggagatggat gcggcttcgc gcaccggcat cgacgacatc
     3001 cgtgaaatca tcgaaggcac gcgctatgcg ccggccgccg cgcgctacaa ggtctatatc
     3061 atcgacgaag tgcatatgct ctcgaagcag gccttcaatg gcttgttgaa gacattggaa
     3121 gagccgccgc cgcatgtgaa attcattttc gcgaccaccg aaatccgcaa agtgccggtg
     3181 accgtgctgt cgcgctgcca acgcttcgat ttacggcgca tcgagtcggc cgcactgagc
     3241 gcgcatttgg cgcatatcgc cggtgaggag cgcatcgcca tcgagtccgc ggcgttgtcg
     3301 ctgatcgcgc gcgccgccga aggctcggtg cgcgatgcgc tgtcgctgct cgatcaggcg
     3361 attgcccatg atgcgggtga aaccgatacc atcgaggctg agtccgtgcg ggcgatgctg
     3421 gggcttgccg atcgcgggcg ggtgctcgac ctgttcgaga aattgatggg cggcgaaatt
     3481 ccgctcgccc ttgccgaatt caaatcgctc tacgacgacg gagccgatcc gctggtggtg
     3541 attcaggatc tgctcgaaac cacccatttc ctgacacggg tgaaggtggc gccaggggcg
     3601 gaagggttct tcgacggcgg ctcgggagag gcggcgcgcg cggtcaccat ggcgggcaag
     3661 ctcaatgtgc ctgcgctgac gcgggcgtgg cagatgctgc tcaaagggct gatcgaagtg
     3721 cgcgacgcct cgaaccccta tcccgcggcg gagatggcgc tggttcgcct ggcctatgcg
     3781 gccgatttgc cgccgaccga acggctggtg cgcagcttgc gcgacagcga gcccgcgtcc
     3841 gtgtccccgc cgccggcctc cccgcgcggt ggatcgggtg gcagcccaag ggcccaaagc
     3901 gccgtgcttg catccgaaga gcccaaaccg ctgcatgacg agatacccgc gtccgccgct
     3961 tccgcgccgc ggatggaaat ccgcaccctc gaagatattg tggcgctggc caaagaccgg
     4021 ggcgcgcgtc ttcttgcgac gcaattggag aacaatgtgc atctggtctc gctggagcgt
     4081 gggcgaattc aatttcgtcc cagcgcgcag gcaccgacgg cgctcgcggg cgatatcgcc
     4141 cagcgtttgc gcgattggac gggcgaacgc tggatcgtca cgctggcatc cgaaggcgga
     4201 gcgccgacca tagccggtca acggatcgcc gacgaacgcg ctaagaaaga tgcggtggcg
     4261 caggagccgt tcgtgcgggc cgtgctcgat gcgtttccgg gcgcggagat cgtcgccgtg
     4321 cgcgatcgcg agacacccga gccggcctca atggcatctg acgaggagca gaactgatgg
     4381 attccaatct gctgcgtcag atgcaggaga tgcagcgccg tgccgacgag ctgcaaaaag
     4441 gtctcgccgc gatggaagtc gagggcgttt cgggcggcgg acttgtgcgc gcgacggtca
     4501 acggcctcgg cgagttgaag gcattgtcga ttgattcttc gctgctcaag cccgaggaaa
     4561 aaggcatcgt tgaggatctg gtggtggccg cctgcgccga cggcaaaatg aagctggaag
     4621 cgcgccggat gcaggattct caattcctgc aggatttgct gaaaagcttc agcgccggtt
     4681 tgccggattc ggattgaggc atggccgggt cgcctgcggg acacgagatc gagcgcctga
     4741 tccaattgct ggcgaagctg ccggggctgg gtccacggtc ggcgcgccgc gccgcgctgg
     4801 cgcttctgaa gaaacgcgaa accctgctcg aacctttggc cgatgcgatg gcaaaagcag
     4861 cttccgcgct gaagcaatgt tcgcattgcg gcaatctcga caccaccgac ccctgcgcgt
     4921 tgtgccgcga tcccgcgcgt gagaccggga tcatctgtgt tgtcgaggac gttgcggatc
     4981 tgtgggcgct ggaacgcgcg agcgtatttc gtggccgcta tcacgtgctg ggcggcgctt
     5041 tgtcggcgct cgacggtgtt acgcccgaac ggctcaatct tcccagcctg ctggagcgcg
     5101 tgaaggcggg ggcgggcgaa atcatcctcg ccatgaacgc gacagtggag ggacaaacca
     5161 ccgcgcatta tctgctcgat acgctggagc cgctgggcgt gaagatcaca cggctcgccc
     5221 atggcgtccc ggtcggcggc gaattggatt atctcgatga aggcacgctc tctgccgcct
     5281 tcaaggcccg ccgcgctttc tgaaaagaag acccttccgc gacggtgcgt tccatcgcgg
     5341 aagggtaagt ccatgggatc ggtgaattct attgggccgg cgcggtttcc gcaggttgct
     5401 ccggcgcgac cggagcctcg ggcatcggcg ccgttaccgg cgcgctatcc tgcgcagatt
     5461 ccgggatcgc cgcaggcgcc gctggttcgg gagcgcttgc aggcgccgca gcttcggggg
     5521 ccgcgggaga ttcattgacg gttggctgcg attccggagc cggcggaatt accggagccg
     5581 gggcttggac cgcgcgcgcc actctgaccg gcggcgttat cgaagccggt gggacgtttt
     5641 gcgcatgatt gtccttgttc ggtgcggccg cgggtttttc caccgcagca ggtggcgttg
     5701 cggatggcac atcggcggac gattgctgcg gcgtttcact cggtggtgtg ggcggtacga
     5761 ccgccgtggt cggaagagat ttggagtcta cgatatgctt cgcaacgggg gcggagttcc
     5821 acgtccccgt ctcgtagctg tatgcgccca tcgcgccgaa acccaacgcg acaacggctg
     5881 caacgataat cttggtggta ttgttcgtgg catccgctgt gggagccgtg tctcctcgca
     5941 catccttcat catcgctgtc tctcctgctt gtaatgtcat tttccgtggg agaatacgca
     6001 cgttcctaag ctaggttgcg aaagagaacg aagatgggcg gaaatgcggc agcccgcggg
     6061 tcatgctgtg tcaggacatt ggctgcaaac gaaatatcgt ggcaagtgca cgtctgtcgc
     6121 gtggcgaagc catccgctac acttttgaaa gcacggggag gatattatga aaaactgttt
     6181 tcttgtcgcc gcgatcgcgg gtctgttgtg cgccggtgca ggcaacgccg ccgaaatcaa
     6241 agtcctttcg acgcaggcga ccgaacaatc ctatcgcgag cttgtgccgc aattcgaaaa
     6301 gaccacgggg cacaaagtaa cgacgatctt caccggtacg ctggatgcgg ataagagatt
     6361 ggcggcgggc gaacgctacg atcttctcat catgtcgagc ccatcgatcg acgagcacat
     6421 caaggatgga aagctcgcgc cgggaagccg cgtcgatctg gcgaaatccg gtgtcgcggt
     6481 cgccgtgctg cgcggcgcgc ccaaacccga tatcggttcc gccgaagcgc tgaagaaaac
     6541 tctgctggcg gcaaaaacca tcggctattc caccgggccg agcggaaaca tcgtcatggc
     6601 attgttccag cgtttgggaa tcgccgatca gatcaaaccg aagctgaagc agaccgcaac
     6661 cggtgtgttc gtcggcagca tcatcgcgag cggcgaggtc gaggtcggct tccagcaggt
     6721 gagcgaactc tcgcaatttc ccggcatcga ctatgtcggt ccgctgccga aagagattca
     6781 gcaatacacc atattctcaa gcggcatcgt tgctggaacg ggtcaagccg atgcggccaa
     6841 agcgctggtg aaatttctca cttcgcccgc tgcggcgccg gttttcaaga agcggggcat
     6901 ggagcccgct tgatgattcc cccgcttgat gattccacag cggaccgtaa tcgccgcggc
     6961 tacaatagtt ttggccgaag tggatgccgg ttcggcctgg aaaatgtgac cggacaaaac
     7021 cgggagcgtg atttccaatc gaacgtaaac ccatcacgct ctagggtctg tacccacata
     7081 gatcgttgaa aattttgacg aacagtgatt caccgtgatg ggaggtggat catgcgattt
     7141 gatttatccg atgaggaatg gacgctcctt gaaccgttga tgccgaagag ccgcaagagc
     7201 gcccgggccg acgaccgcaa gat
//
LOCUS       DASYEG010000081         2836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1619240, whole genome shotgun sequence.
ACCESSION   DASYEG010000081 DASYEG010000000
VERSION     DASYEG010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619240"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1767)
                     /gene="asnB"
                     /locus_tag="VGM72_03020"
     CDS             complement(<1..1767)
                     /gene="asnB"
                     /locus_tag="VGM72_03020"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEY4264261.1"
                     /translation="MCGIAGVMTSDATHPKPAMLDGFLRALAHRGPDGEGRYEAPGVG
                     LVQTRLAIIDLSTGDQPLYGDNGTALVANGEIYNYLELKRDFPTTHFKTHSDCELPLA
                     SYARDGADFAKPLRGMYAIALHDARDHALYLARDPFGIKPLYYAEIPGGIVFASELQA
                     ILKTNFVTAKIRPQTATELVQLQFTTGRDTIFEEIHRVMPGETLTLRGGRIVGRRQKP
                     ALPEGPPLKLGEVEALKALDAALMDSVSVHQRSDVPYGMFLSGGIDSAAVLACMARLS
                     EKPVRAFTAGFPATATHDERENARRVAASVGAEHIEIAITDKDFWTQLPAIAAIMDDP
                     AADYAALPTFLLAAQAASELKVVLTGEGGDELFAGYGRYRSALRSPFLGGRPMRRRGA
                     LEGLGVMRDSSRQWRAGIATAERRLSSSNSTRLQQAQALDCADWLPNDLLLKVDRCLM
                     AHGLEGRTPMLDPVVADLAFRLPDDLKIAKGKGKYLLRRWLQDALPVADPFAEKRGFT
                     VPVAEWMQPSAAKLAPLLSRSAGLAEICYPEKIGALFAAFGNLGGKHEGIACWQLLFY
                     ALWHAIHVEKRPIVHNVFAMLEA"
     gene            complement(1767..2795)
                     /locus_tag="VGM72_03025"
     CDS             complement(1767..2795)
                     /locus_tag="VGM72_03025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4264262.1"
                     /translation="MALKILTVLAGASVGGAETFFVSLTLALAHAGAAVHSVLKSNAL
                     RERALKQAHISYDTAPFASPVDFTSTRKLRRAVAAFRPDIVLAFAGRAASFVPRGNYT
                     IIGRLGGYYNLRNFKGCDYLVCNAPDLVRYVRAGGWTESKVTLIPNFPSVPDEPAADR
                     ALFDTPAEAPLAVALGRLHPNKGLDVLIRSLASVPDLFVWIAGEGPERVALENLARDC
                     GVAQRIRFLGWRSDRAALYKAADLCVYPSREEPFGNVVVEAWSCGVPIVTTASVGPSW
                     LARNGEDAIITPIDDPAALADAVRTLIASKELRARLVAGGKKRVAEEFSEGAIVKRYF
                     DLFEKVAR"
BASE COUNT          577 a          885 c          862 g          512 t
ORIGIN      
        1 ggcctccagc atggcaaaaa cgttgtggac gatagggcgt ttctcgacgt gaatggcgtg
       61 ccacagcgca tagaacagaa gctgccagca agcgatgcct tcatgcttgc cgccgagatt
      121 gccaaaggcg gcgaagagcg cccctatttt ttccggataa cagatttccg ccaatcccgc
      181 cgaacgcgac aaaagcggtg ccaacttggc ggcactcggc tgcatccatt cggcaaccgg
      241 cacggtgaaa ccgcgttttt ccgcgaaggg atcggctacg ggaagggcat cttgcagcca
      301 tcgccgcagc aaatattttc ctttgccctt ggcgatcttg agatcgtccg gcagccggaa
      361 agcaaggtcg gcgacgacgg gatcgagcat cggcgtgcgg ccctcgagtc cgtgcgccat
      421 caggcagcgg tcgaccttaa gcagcagatc attcggcagc caatccgcgc aatccagcgc
      481 ctgggcctgc tggagccgcg tagaattgct gctggagagc cggcgttcgg ccgttgcaat
      541 gcccgcgcgc cactggcgcg aggaatcgcg catcacaccc agtccttcaa gcgcgccgcg
      601 ccttcgcatg ggccgcccgc cgaggaaggg tgaccgcagc gcgctgcgat agcggccata
      661 acccgcaaac agctcatcgc cgccttcgcc cgtaaggacc accttcaact cgcttgcagc
      721 ttgggctgcc aacaaaaagg tgggcagcgc cgcgtaatcg gctgcgggat cgtccatgat
      781 ggcggcgatt gcgggaagtt gcgtccaaaa atccttgtcc gtgatagcga tttcgatgtg
      841 ctccgcgcct acggaggccg ccacgcgccg cgcattctcg cgctcatcat gagtagccgt
      901 ggccggaaag ccggccgtga acgcgcgcac gggtttttcg ctcagccgcg ccatgcaagc
      961 gagcaccgcc gcggagtcga tgccaccgga cagaaacatg ccatagggca catccgagcg
     1021 ctgatgcacg gagacgctgt ccatcaacgc ggcatcgagc gctttcaagg cctcgacctc
     1081 gcccagcttc aacggcgggc cttccggcaa tgccggcttt tgccggcggc ccacgatccg
     1141 tccgccgcgc aatgtcaatg tttcacccgg catgacgcga tgaatttctt caaagatcgt
     1201 gtcccggcca gtcgtgaatt gcagctgcac gagttcggtc gcggtttgcg gacgaatttt
     1261 cgcggtaacg aaattggtct tcaggatagc ctgcaattcg gaggcaaaaa cgattccgcc
     1321 aggaatttca gcataataaa gtggtttgat tccgaacggg tcgcgcgcga gatagagcgc
     1381 atggtcgcgc gcgtcgtgca acgcgatggc atacataccg cgcagaggtt ttgcgaaatc
     1441 agcgccatcg cgcgcgtagc tcgcgagcgg aagctcgcag tccgagtgtg tcttgaaatg
     1501 ggtggtggga aaatcgcgct tcagttcgag ataattgtag atctcgccat tggcgaccaa
     1561 tgccgtcccg ttgtctccat acaggggttg gtctccggtg gaaagatcga tgatcgccag
     1621 ccgcgtttgc acgaggccca cgcccggtgc ctcataacgg ccttccccat cgggcccgcg
     1681 atgggcaagc gcgcgcagaa acccatccag catcgccggt ttggggtgcg ttgcgtcgct
     1741 ggtcatcacg ccggcgatgc cgcacatcag cgcgcgacct tttcgaagag atcaaaatag
     1801 cgcttcacga tggcgccttc cgaaaattct tcagccacgc gctttttgcc gccggcaacg
     1861 aggcgcgcgc ggagttcctt cgacgcgatc agcgtgcgga cggcatcggc cagcgctgcc
     1921 ggatcgtcaa tcggggttat gatcgcgtcc tctccattgc gcgcgagcca ggaagggccg
     1981 acgctggcgg tcgtcacaat gggtacgccg catgaccaag cttccaccac cacattgccg
     2041 aaaggttctt cgcgcgaagg atagacgcaa aggtcggcag ccttgtagag cgccgcgcgg
     2101 tcgctgcgcc agccgagaaa tctgatcctt tgggctactc cgcaatcgcg cgccagattc
     2161 tccaacgcaa cacgttcggg cccctccccc gcgatccaaa cgaacaaatc cgggaccgac
     2221 gcgagggagc ggatcagtac atccagcccc ttattgggat gcaggcggcc aagcgccaca
     2281 gccaagggcg cctccgccgg cgtgtcgaaa agagcccggt cggctgcagg ctcatcggga
     2341 acggacggaa aattagggat cagcgtaacc ttcgattccg tccagccgcc cgctctcaca
     2401 tagcgaacaa ggtccggggc attgcagact aggtagtcgc aacccttgaa attcctcaga
     2461 ttgtagtaac cgccgagccg cccgatgatt gtgtagttcc cacgcggaac gaacgacgcc
     2521 gcccggccgg cgaaggcgag cacgatgtcg ggtctgaacg cggctaccgc acggcgcaat
     2581 ttgcgcgtgc tggtgaaatc cacgggactt gcgaatggcg cggtgtcata ggaaatgtgt
     2641 gcctgtttca gcgccctttc ccgcaatgcg ttcgacttca ggaccgaatg gactgccgcc
     2701 ccggcgtgcg ccagggcaag cgtcaggctg acgaaaaatg tttcggcgcc gcctacggat
     2761 gctcccgcca gcacggtgag gattttcagc gccatggaac cttcttcaag gggacgtcag
     2821 aagtacgaca gcccat
//
LOCUS       DASYEG010000082         5616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_458905, whole genome shotgun sequence.
ACCESSION   DASYEG010000082 DASYEG010000000
VERSION     DASYEG010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458905"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2455
                     /gene="uvrA"
                     /locus_tag="VGM72_03030"
     CDS             <1..2455
                     /gene="uvrA"
                     /locus_tag="VGM72_03030"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY4264263.1"
                     /translation="RILALPEGTRLFVLAPIVRGRKGEYRKELADLQKKGFQRVKVDG
                     KIHELADVPPLDKKLKHDIDVVVDRVAVRADLGNRLPDSIATALDLAEGLLVIEFADQ
                     KEKGEPKRLVMSSKFACPVSGFTIPEIEPRLFSFNNPHGACPECDGLGSKLYFDPAMV
                     VPDESKTLREGVIAPWSRSSSPYYLQTLEAITRHYKAGVNTPWEDLPKKIRDAILFGS
                     GDEEIKFSYDDGARRYDIKKAFEGVIENMERRYKESDSAWVREELEKFQSESPCEVCK
                     GYRLKPEALCVKIAGNHIGQVCELSVRAADRWFAELDAQLNPKQKEIAARILKEIRAR
                     LLFLNNVGLEYLTLSRSSATLSGGESQRIRLASQIGSGLSGVLYVLDEPSIGLHQRDN
                     ARLLDSLKGLRDLGNTVIVVEHDDEAIRTADYVFDMGPGAGIHGGHLIAEGVPDAIMR
                     NPNSITGQYLSGARQIPMPAMRRRAAHNRWLKVVGATANNLKNVTAEVPLGTFTCITG
                     VSGGGKSTLTIETLYKAVARRLNNAREHPAPHEKILGLEFLDKVIDIDQSPIGRTPRS
                     NPATYTGAFTPIREWFAGLPEAKVRGYGPGRFSFNVKGGRCEACQGDGVIKIEMHFLP
                     DVYVQCDVCNGKRYNRETLEVKFRGFSIADVLEMTVEAGADLFKAVPAVREKLDTLTR
                     VGLGYIKVGQQATTLSGGEAQRVKLAKELSRRATGRTLYILDEPTTGLHFEDVRKLLE
                     VLQELVESGNTIIVIEHNLEVIKTADWIIDLGPEGGEGGGEIIAAGTPEDVAKVARSY
                     TGRYLKPLLKKQIRANAAE"
     gene            2666..3289
                     /locus_tag="VGM72_03035"
     CDS             2666..3289
                     /locus_tag="VGM72_03035"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY4264264.1"
                     /translation="MGTGDVTYDIQTWAHTTERRKFCRVRISAQAKLFVTGDSRETGC
                     TVMTLSPGGAGIRCKNPPPARTHIVLYINGFGRFEGVTTKLTRDGIGVRFDCTPRKRQ
                     RTVEQLTSFVKEGLATGIMQLSYYNRAALEVSGAEPPEETAEITSAMLSAGQAEARSW
                     KDPNPHAPHILMAVYLAMARMDGRAQDVQDQPKLLPAPRKRAGGRDE"
     gene            3589..3930
                     /locus_tag="VGM72_03040"
     CDS             3589..3930
                     /locus_tag="VGM72_03040"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY4264265.1"
                     /translation="MMTDAAMALPPRAGDSRKFLRQRVYLSGRIIYGDGSFTINCRIR
                     DISECGARIVLSVGMVIPTHNILVQVGNRIVHDAAVARIASPEFGLKFISTHSLDAVL
                     PPHMQYLKRYR"
     gene            4027..4767
                     /locus_tag="VGM72_03045"
     CDS             4027..4767
                     /locus_tag="VGM72_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264266.1"
                     /translation="MTRLPNPVWPCLLMCLPIWLMQAPPAAAEVLDKTASINGLTLHY
                     KVVLPKSFDPAKSYPAVLAFPPGGQDMEMVDATLSQNFRDQAEQRGYIVIEPAAPNGT
                     LFFEGGERVFPAFLIKLTGDYKILDNKFHVAGNSNGGLSAFLIASKYPQFFWSVTGFP
                     GYLDSGTPQQMAALGKMCIHMFAGERDSGWPEAMRDQAAKFRSLGYNVTFAIEKGQPH
                     VIRTLTGAGAARLFDQFDAARTGHCAQG"
     gene            complement(4807..>5616)
                     /locus_tag="VGM72_03050"
     CDS             complement(4807..>5616)
                     /locus_tag="VGM72_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264267.1"
                     /translation="AASLAAVVVIPAMLLFGKFPGTTNAASAPVATLSIPTQAIPRQA
                     SVTPPPSTPVPPSGSHTAIVNNDLAAAEKSDTGNSTRKSASLSRTPDAAKPSGQVRTA
                     KPAKPANDAKAAKATPAPVPSAAAKAPATPSPAVVAPAPVQAADGAPAPSEAAVQAGT
                     APAATVNPANAAAEAEARSVAVAAEVARIMAAQQEAEREKAAKDASAQAQAAAIRASV
                     ARDAANRINAAMQEAQAAQAATQAAAAAQAAKQTAEKERRAFQNSLFGVAH"
BASE COUNT         1058 a         1739 c         1758 g         1061 t
ORIGIN      
        1 ccgcatcctg gcgctgcccg agggcacgcg gcttttcgta cttgccccca tcgtgcgcgg
       61 ccgcaagggc gaataccgca aggaactggc cgacctgcag aagaagggct tccagcgcgt
      121 caaggtggac ggcaaaatcc acgaactcgc cgatgtgccg ccgctggaca agaaacttaa
      181 gcatgacatc gacgtggtgg tggaccgtgt tgcagtgcgc gccgacctcg gcaaccggct
      241 gccggattcc atcgcgacgg cgctcgatct ggccgagggc ctcctggtga tcgaattcgc
      301 cgaccaaaag gaaaagggcg aacccaagcg gctggtgatg tcctcgaaat tcgcctgccc
      361 ggtgtcggga ttcaccattc cggagatcga gccgcggctg ttctcgttca acaatcccca
      421 tggcgcctgt cccgaatgcg acgggctggg gtccaagctc tatttcgatc ccgccatggt
      481 ggtcccggat gaaagcaaga cgctgcgtga gggcgtcata gcaccgtggt cgcggtcatc
      541 ctcgccctat tatctacaga ccctggaagc gatcacgcgc cactacaagg ccggcgtgaa
      601 cacgccttgg gaggacttgc ccaaaaaaat ccgcgacgcg atcctgttcg gctcgggcga
      661 tgaggagatc aaattctcct atgatgacgg agcgcggcgc tacgacatca aaaaggcctt
      721 cgagggcgtc atcgagaata tggagcggcg ctacaaggaa tccgattccg cctgggttcg
      781 cgaggagctg gaaaaattcc aatcggaaag tccatgcgag gtgtgcaagg gctatcgcct
      841 caaacccgaa gccttgtgcg tgaagatcgc cggcaatcat atcggccagg tgtgcgagct
      901 ttctgtgcgc gccgccgacc gctggttcgc ggagctcgat gcgcagctca atcccaagca
      961 aaaagaaatc gccgcgcgca tcctgaagga aatccgggcg cgtcttctgt tcctcaacaa
     1021 tgtgggtctg gaatatctga cgctatcgcg cagttccgcc acgctttcgg gcggcgagag
     1081 ccagcgcatc cgcctggcct cgcagatcgg ctcggggctt tcgggtgtgc tctacgtgct
     1141 ggacgaacct tccatcggtc tgcatcagcg cgacaatgcg cggcttctgg attcgctcaa
     1201 aggtctgcgc gatctcggca acaccgtgat cgtggtcgag catgacgacg aggcgatccg
     1261 caccgccgat tatgtcttcg acatggggcc gggggcgggg attcacggcg ggcacctgat
     1321 cgccgaaggc gtgcccgatg cgatcatgcg caaccccaac agcatcaccg ggcaatatct
     1381 ttccggcgcg cgccaaattc cgatgccggc gatgcggcgg cgcgcggcgc ataatcgctg
     1441 gctgaaagtg gtgggagcca ccgccaacaa tctgaagaac gtgaccgccg aggtgccgct
     1501 cggcacgttc acctgcatca ccggggtttc gggcggcggc aaatccacgc tgacgatcga
     1561 gaccctgtac aaggcggtgg cgcgcaggct gaacaacgcg cgcgaacatc ccgcgcccca
     1621 cgaaaagatc ctcggcctgg aattcctcga caaggtgatc gatatcgatc aatcgccgat
     1681 cggccgcacc ccgcgttcca atccggcgac ctatacgggc gccttcacgc cgatccgcga
     1741 atggttcgcc ggtcttccgg aagccaaagt gcgcggttat gggccgggac ggttctcgtt
     1801 caacgtcaag ggcggacgct gcgaggcctg ccagggtgac ggcgtcatca agatcgagat
     1861 gcatttcctg cccgacgtct atgtgcaatg cgatgtctgc aacggcaagc gctacaaccg
     1921 cgaaacgctg gaagtgaagt tccgtgggtt ctccatcgcc gacgtgcttg agatgacggt
     1981 ggaagccggt gccgacctgt tcaaggcggt ccccgcggtg cgcgagaagc tggatacgct
     2041 gacgcgcgta gggctaggct acataaaggt cgggcagcag gcgacgacat tgtccggcgg
     2101 cgaagcgcag cgcgtgaagc tcgccaagga attgtcgcgc cgcgccacgg gacgcacgct
     2161 ctacatcctg gatgagccga cgacgggctt gcatttcgaa gacgtgcgca agctgctcga
     2221 ggtgctgcag gagctggtgg aaagcggtaa cacgatcatc gtcatcgagc acaatctcga
     2281 ggtgatcaag accgcggact ggatcatcga cctggggcca gagggcggcg aaggcggcgg
     2341 tgagatcatc gcggccggca cgcccgagga tgtcgccaaa gtggcacgct cctatacggg
     2401 acgatatctg aagccgctgc tgaaaaaaca gatccgcgcc aacgcggcgg aatgaaggct
     2461 tgattgtcgt ggttacggca ttgctaatcc gcgcatagct ggcaccggca ttgcaaatcg
     2521 tccaccatcg ggccgaacgt gtgtgccgtt tcgatacgct ccacacggca cgtcccggta
     2581 gcgatgcaag tgagtttaca acgtctttat ggttgcttcg tagacttcgc cggaacgttc
     2641 ttgcgacttg gtctgcgtct cattgatggg taccggcgac gtgacttacg atatccaaac
     2701 ctgggcgcac acaacggagc ggcgaaagtt ctgccgcgtg cgtatctcgg cgcaagccaa
     2761 gctcttcgtc accggcgatt cccgcgaaac cggctgcacg gtgatgaccc tgtcgcccgg
     2821 cggcgccggt atccgctgca agaatccacc gccggcgcga acgcatatcg tgctctacat
     2881 caacggcttc ggccggttcg aaggcgtgac caccaagctg acgcgcgatg ggataggcgt
     2941 tcgcttcgat tgtacgccgc gcaagcgcca gcgcacggtc gagcagctga cgtccttcgt
     3001 caaagaaggc ctcgcaaccg ggatcatgca attgagctat tacaaccggg ccgcccttga
     3061 ggtctccggc gcggagccgc ccgaagaaac ggcggagatc acatccgcga tgctgagcgc
     3121 cggtcaggcc gaagcacgtt cgtggaaaga ccccaatccg catgcgcccc acattttgat
     3181 ggcggtctat ctcgccatgg cgcggatgga cggccgcgcg caagacgtcc aggatcagcc
     3241 caagctgctc cctgccccgc gcaaacgcgc cggcggccgt gacgaatagg cagtatttta
     3301 ttttgccgtg cggtctttat ttccgcatct ttctaaattt tcgccctgaa cattccctac
     3361 aatatgtctc gaaattgcac gcgctgccgc tcagcgtgag acagacgcgc cgcgcgcgga
     3421 cgcacgccgc gccggatttt gtggacagcg gcgcaaaatc cctgcgacgg cattctggca
     3481 tgggacgtgc aaatcacctc gtgacgttca aagtgcgccg gagctttcgg tgaattgaca
     3541 gggcatctgc aaatggagac ctcctatcgt gggccttcgc gttcttccat gatgaccgac
     3601 gctgcgatgg cactcccgcc tcgcgccggc gattcgcgaa aatttctgcg ccagcgcgtt
     3661 tatctctccg gcaggatcat ctatggcgat ggctcattca ccatcaattg ccgcatcagg
     3721 gacatttccg agtgcggggc acgcatcgtg ctttccgtcg gcatggtcat tcccacgcac
     3781 aatatcctgg tgcaggtcgg caatcgcatt gtccacgatg cggcggtcgc gcgaatcgct
     3841 tcgccggaat tcgggctgaa attcatcagc acgcattcgc tcgatgctgt cctgccgccg
     3901 catatgcaat atctgaagcg ataccggtag ggttctgccg cgggcgggcg tgaatatttg
     3961 acggcggcgt ctactcccct tctcgatgcg gcgctagact tgcgcgctct cgagaaggag
     4021 accgcaatga cccgccttcc caatcccgtt tggccctgtc tgctgatgtg tcttccgata
     4081 tggctgatgc aggcgccgcc tgctgccgcc gaggtgctcg acaagaccgc ttcgatcaac
     4141 ggcctcacgc tgcattacaa ggtcgtgctt ccgaaatcgt tcgatccggc aaaatcctat
     4201 cccgccgttc tggcgtttcc gcccggcgga caggacatgg agatggtcga cgccacgttg
     4261 agccagaatt ttcgcgacca ggcggagcag cgcggataca tcgtgatcga gccggccgcg
     4321 ccgaacggga ccctgttctt cgagggcggc gagcgcgtgt ttccggcatt cctgatcaag
     4381 ctcaccggcg attacaaaat cctggacaac aaattccacg ttgcggggaa ttccaatgga
     4441 gggctcagcg cattcctgat cgcatcgaaa tatccgcaat tcttctggtc cgtgacgggc
     4501 tttcccggat atctcgattc cggcaccccg caacagatgg cggcactcgg caagatgtgc
     4561 atccacatgt tcgcgggcga acgagattcg ggctggcccg aggcgatgcg cgatcaggcc
     4621 gcgaaatttc gtagcctcgg ctacaatgtg accttcgcca tcgagaaggg gcaaccccat
     4681 gtgatccgca cgctcaccgg tgcgggcgct gcgcgcctgt tcgaccaatt cgatgcggca
     4741 cggacagggc attgcgccca gggttgagcg gctgtgctca tctgcatttg ggaaatcccg
     4801 gacgtcctaa tgagcgacgc cgaataggct gttctggaac gcgcgccttt ctttctcggc
     4861 agtctgtttc gccgcctggg cggcggcggc agcctgggtg gcggcctgcg cggcttgggc
     4921 ttcctgcatc gcggcgttga tcctgttggc cgcgtcacgc gcaacgctgg cgcggattgc
     4981 cgccgcctgc gcttgagcgg acgcgtcctt ggcggccttc tcgcgttcgg cttcctgctg
     5041 cgcagccatg attctcgcca cctccgcggc gacggccacg gaccgggctt cggcttccgc
     5101 cgcagcattg gcgggattga cagtcgcggc cggagcggtc ccggcttgaa cggcggcctc
     5161 actcggggcc ggagcaccgt ctgcagcctg aaccggagcc ggagcaacca ccgccggtga
     5221 cggcgtcgcg ggcgccttgg cggcggcgga cggcaccgga gctggcgtgg ccttcgccgc
     5281 cttcgcgtca ttggcgggtt ttgcgggttt ggccgttctc acctgaccgg aaggcttcgc
     5341 ggcatccggg gtcctggaaa ggctcgcgct tttccgggtg gaattgccgg tatcggattt
     5401 ctccgctgcc gccaaatcat tgttcacgat cgctgtatgc gaaccggaag gcggcaccgg
     5461 cgtggacggc ggcggcgtga cgctcgcctg cctcggaatg gcctgcgtcg gaatcgacaa
     5521 cgtcgcaacc ggcgcgctgg ccgcattggt tgtgccgggg aatttgccga acagcaacat
     5581 cgcggggatc acaaccacgg cggcgaggga agcggc
//
LOCUS       DASYEG010000083        17878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4518636, whole genome shotgun sequence.
ACCESSION   DASYEG010000083 DASYEG010000000
VERSION     DASYEG010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 17878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17878
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4518636"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="VGM72_03055"
     CDS             complement(<1..1042)
                     /locus_tag="VGM72_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008439522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY4264268.1"
                     /translation="MSISHHSQALPAPLTGTSSDDTSLLGGNERMLLGLRLTLALAAF
                     CLLAVAGAIRLFLPAQTGISDLVAGVAAAMVAVPVFSEAWASLRHPSLHGITDRLVAL
                     ALVACWATGDLLTAAFLPIVMTVGHILEERSMLGSREAVQALGKLTETDARRLLPDGS
                     VEMVPTANLRTGDLIELRAGERLPADGTVRRGASSLDMASLTGESVPVEVNEGVTVLA
                     GAMNIDGLLQIELTRVGEDTTLGRIILLMREAENAKPPVTRLLDRYAGQYMTLVLLVA
                     AGTWFVTGNVPAMLAVLVASCPCALVLAAPATAIAAIVVAARHGILIKGSAFLEQLAD
                     VTAVVFDKTGTIT"
     gene            complement(1039..2145)
                     /locus_tag="VGM72_03060"
     CDS             complement(1039..2145)
                     /locus_tag="VGM72_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY4264269.1"
                     /translation="MNDLSDEIQRARGEIGRGDIGPPPEPPPGPIAQSVAIGFRAVYV
                     VTLLLAVVWLFSNVREIAPDNQAVVLRFERIVRVQNSGLLLAWPRPIEEVRLLPGPDR
                     QLNKSVGALPPVGGIARPSAQDAAAAANIPATASPYLTGDGNVVFLDARLVYRISDPR
                     AYVLSEAHVAPALDRLFRATAVNVTAGWGLNDFLVAQPNTAGGAQGQAIVALRTAVRD
                     DLLKGINAKLQQLNAQDVGLGVEVAQIDLTAWLPPEAKVAFDAVLTATQAADQQVAAA
                     RTAAELRRQGAEREGDRVKSAAEATASERVTTATVDTATIKALVAQTTAQTRQSVMLR
                     AYNERVADIMKRTGSVTLVDPQSGARFVLPGKTP"
     gene            complement(2142..3146)
                     /locus_tag="VGM72_03065"
     CDS             complement(2142..3146)
                     /locus_tag="VGM72_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEY4264270.1"
                     /translation="MFSHHHPDDHDDHDDDHDHGPEHERRERIALGLRIAVAAFVLIA
                     AISAACFVMVPAGEAVVVTRFGNPVRVITEPGLRLKLPIPIEMTIPINLRLRTTSSGL
                     QDVGTKDGLRVLVQTYAAWKVSSDPEHIRQFLRSVRNEPDEAAVQLRSFISAQLHITA
                     SNFDLADLVNIDPAKVRVPAFEQQLRDQVAQRMLQVYGIEISQVGVERLTLPTETLAA
                     TVARMRAERETVAAERTAEGLRQAAIIRADADRDGRQVVAKAREQAAEIEANAEQAAA
                     KIHADAYRGDPQLYNMVRSLDTVSKVVNRSSAMVLRTDAAPFRALVTGVPGTGAGAAP
                     "
     gene            complement(3205..5214)
                     /locus_tag="VGM72_03070"
     CDS             complement(3205..5214)
                     /locus_tag="VGM72_03070"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY4264271.1"
                     /translation="MAQADHEIGGLGLGSSQAYRFVTRLLWLYGGFTFVVVLGLVQAW
                     LPVTVLRTQAQEAIAGGGGGLVLATLLMALTGLFGAQLLAAARQRHFAVEEIVNEPAG
                     RRFVLRQIGPGIAARQGQAFLIATGAALTCFASWLLWPMARTLPSAMADPNLVGAIAI
                     ALAFCSLIGERMMAVFPVPQMPEATALRRLLLVTTILLAAAGALEIGRGAGQLWVYWI
                     FAALSVVPWIVTIELALRALARLFLPVPQPLEARAAVDSIFLGLVTGGPRAPGMLIRE
                     HLGLDFTRSWALSYLTSAALPAVLATALLCWGLSGLKLIDISQRGIYERFGAPVAVLG
                     PGIHLLLPWPLGIMRPVEYGTLHEVKVGSSVPEATLVRIDAEAVPPPSTNHLWDTADP
                     AEAEYLVASQSGAGVQGFQVVDAEIHVVYRTGLSDADAMAAVYGAADPGTLVGEASSR
                     VVSMYFASHTLDTVMGAQRDMLAELLRANVAREVAAYHAGLEILSLHIESVHPPVGAA
                     AAYHAVQAAEINANASVFNETGRAKRMAGVAQEEAHQMTSAAEASAEEKIQSAKGETY
                     QFAADRKAYAEGKESFVTERTFQALVAALSQVRLTIIDNRLTPAQAPIIDMRGGNAAL
                     PAPATAPVAGSVPGAPPAAAAVPPPARPPAPEGPRPLTPGVEDAN"
     gene            5405..6178
                     /locus_tag="VGM72_03075"
     CDS             5405..6178
                     /locus_tag="VGM72_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY4264272.1"
                     /translation="MIGRGYRLWVVLGLLIAAGGAVFAWRQTQTNRLETQVLTLLPDE
                     VPRHADLVNLSIAEAKPVYAAHCASCHGADMKGDPRIGAPNLTDGVWLYGSGNVTDIE
                     RTILYGIRSGHGQSHNVTEMTAFGLSGQLTPGEINDVVQYVLSLSKRPNTAQAALSGS
                     QIFAGKGNCGDCHGPDGRGDPYYGSSNLTVNVWNNGGDTASLYRSIYFGLQRVCPAWY
                     GIIPLEKIRALAVYLHAVSHSEAGAESGAASGAAAPPAS"
     gene            6204..7151
                     /locus_tag="VGM72_03080"
     CDS             6204..7151
                     /locus_tag="VGM72_03080"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY4264273.1"
                     /translation="MDMKTPRPPSLPSRLFGGLRYRPPRGVAVPLAGAFAGLAIALFN
                     LLGPGSRPVSVVPAGYAALVNQRPILMSDFINQTETIESTPFAETTPEQRRAVLHSMI
                     DEELMVQRALVLDLPEQDTDVRKELVDAVGAVILARKTPTDADLMAYFNAHRSNYAGV
                     GEMRFHDIVLHFGGSQDADQSLEQAEADAHEAVYQLRSGAPLESVMQHFGFVDSGKTD
                     STMDDLDFAVKLHLGEKLFNVAAKLTDGEVSEPVDEVDGVHVLVMEERKPPRPAEFAV
                     SRNQVYTDYRRDQQLRAEQDNLKFLRANAQILLAPGQSQ"
     gene            7162..8376
                     /locus_tag="VGM72_03085"
     CDS             7162..8376
                     /locus_tag="VGM72_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264274.1"
                     /translation="MNRLFVLLCLLLLAAGPAGAETRSASYGLWNVTGGTVRILYTLP
                     DAEAKRLAAPGAPVLTTKQVADYILAHMGVSHRGKACPIVDQGEDLGLINTLALTPGF
                     LRFEMVFQCPDGAGLTLSDTAFADRIANHADYGRVQINNGGLVQHVFTAAAPRFALPA
                     PGGTLQGDSIFRYAQLGFAHVANGVDILCFTLALLLIARRRRDFALAFGALAAGYGTA
                     ALLGSFNLAAVRFTDGEAFSGMLVLAAAAAGLALSRPDPRRAALGLSSGALVLGAAAW
                     FFHGAAAAFAVLGIALLAIATLYVPARDERRALFLVLASFLFALLDGFTLAGDVAILF
                     LSPAQLAPMLVGFNAATLAGSLVLPLCAMAAWLLVPLFRRLTVPRGLAAELAASVFIG
                     FGIFGFATWLYS"
     gene            8435..9430
                     /locus_tag="VGM72_03090"
     CDS             8435..9430
                     /locus_tag="VGM72_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY4264275.1"
                     /translation="MIRRILRTSMSVRIAVLVCGLAPLLIARELNAQAELTVPAGLPD
                     WTFNIPDKAQPSAVRVEGIVKAPGSTKEYEAAKIAGNANPPDWFPDEHPAAPHVVTGG
                     QGVRFACGSCHLMSGQGHSEAADLAGQPAEYLIRQMAYYKTGARKEDARMGPIAKITS
                     DEDVRQAADYFAALKPRVWVKVIETATPPKTFIATAGRHRQLLPGGGTEPIGHRILEI
                     PADPFLTEIRDPHSGFIAYVPPGSIARGEALVKDGASGKTVQCARCHGEALKGKGDVP
                     RLAGLQPLYVARQLFDMRYGSSAGETAAPMKPVVANLSEDDIIAISSYLGSLPPQ"
     gene            9827..12223
                     /locus_tag="VGM72_03095"
     CDS             9827..12223
                     /locus_tag="VGM72_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4264276.1"
                     /translation="MSSVRHSWIRSIFLGGTAVSVGMLICANRASAQPAPQAQPVQVA
                     QDAVPETVIVTGTLFDPDVAPAKASLDTKEPQTIINRSYIEDSVSPTADYVTILAITP
                     SLTGADINGPGLSDGNVKNTLRGLPDGQYGMTYDGIPFGDTNGPTHHSASYFPATTIG
                     SIEVERGPGNAGNLGPATFGGSINLYSEPLADGMRAKGSVSFGSFGTTDQNLNFQSGN
                     INIGDISSTRILFNFQNTDSDGALTLQRLRRNNDLLKIENNLSANWTLTFFANYSKLT
                     ENLDDNNGATLAQVATYGKDFALQSSNPNLPTYYQYNVTNKSTDLDYIRLKGNIADDF
                     KVDETVYTYAYVNKTMSPRSILQTITDINNGTAQGIGGKFNPIVGGAVQTNDIPGYQK
                     LNAYRVWGNIIRGSADYQIGDISGQVRLGVWWEGAATQRARFDFDSTLCLANGINPWV
                     TFGTSCQDSSLTPKSAVKVTGQGNPTYNGYAELLEHSGWQQYQPFLEVDFRPIEALTI
                     TPGVKYVDWNHTTDSVVEPKLLKPFIGSFRTTRTLPFLEANYKFTNSWSVYAQYAQGI
                     YVPDITAFEQKTAVDAFPKAQTTTNYQVGTVFYADNFTFDADLYYIPVKNNIVFENCS
                     LAGGLAGDTCGVNTGEALYKGIEGETTYAFNDDIMSGALRGLVAFINGSVNSAKSQGF
                     YLKQAPMWTAAAGLVYKRDAFKISLIDKLVGPQYSDNAQNVNYKVHAYNNMDATVGYD
                     FGRFEASVGVYNVLGSRSVLAITENDAAFIADRLLSTDQYYFQAPRSFMVTLKASVSP
                     "
     gene            complement(12248..12655)
                     /locus_tag="VGM72_03100"
     CDS             complement(12248..12655)
                     /locus_tag="VGM72_03100"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEY4264277.1"
                     /translation="MAMEGLITIPSSFTAAETMDRLEAGIKAKGIGVFARIDHASGAA
                     KAGLSLRPTELLIFGKAEGGTPLMQSNQTAGIDLPLKALVWQDASGKVWLSYNDPAWL
                     AKRHAVGIAAEPNVRALAALLDDLFSHSCGVTD"
     gene            complement(12668..14188)
                     /locus_tag="VGM72_03105"
     CDS             complement(12668..14188)
                     /locus_tag="VGM72_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264278.1"
                     /translation="MQDFVPLQEKSCAPNKSWRSKASSRQNAPLQSRSDASPFAADFS
                     RVPIQQQMARGSGPGEGGSKQPSPATDQGPDLPPANPSLEVIAPQTDPAQGAVPMTQV
                     AVGTIKANIGPNGRSIAHVPPCGNQPAIQFTAGPATAKPVTWTIDAGSPAAGTALTPG
                     ADTLTAALSLGAAQKGGVLDIKAENNEGGQVMPYRLASHPTGIASTNVIGDPTDSSLF
                     GGVFDHVFKSNDGQVTSLDQVVMGERFPKLATPEAATHKMTTPFGEFTLQTGTLPDIP
                     AKGNWFMNDSGELGDNHDTVGIQKSLIDVGRHLASDSNPTPANPMPVGFTADQEFHWW
                     CPHGAAGSRWNSANSTTTHERTLRLDKSGKNAEFVAKVNGKENVMAYDGDPKIVLTGV
                     THAKADHAAVETSAAGRSANTIQISADLFPSSRDAHFSIRGNALGCKIDASSGELTIG
                     SKPGTVKVRVANAKDGPNWDEVDVTVTAPAAPKTTPANATSVPAPRAGADAPPSDL"
     gene            complement(14259..14954)
                     /locus_tag="VGM72_03110"
     CDS             complement(14259..14954)
                     /locus_tag="VGM72_03110"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HEY4264279.1"
                     /translation="MTELETEDITCELVIKPALYLMEAHLPASLVTTINDHVDGVRDS
                     AADHSPSLVGQIRQHARSAQLQLDLRQKAPATLAAVIVEIAGQYVAQQGYRAAVTAHD
                     MWSVHSYAGDYNPLHDHGGNTPLGLSSILYLKVPSGIAEKPAVQHGGSPKLNLTSGNC
                     DGFTQFVWGGNGMRDFSLLRPPTQQYIKPETGRLIVFPNWLLHRVEPFFGEGERRTLS
                     CNMDVVFDEPDWY"
     gene            15204..16823
                     /locus_tag="VGM72_03115"
     CDS             15204..16823
                     /locus_tag="VGM72_03115"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY4264280.1"
                     /translation="MADIAASPETFRGRGTPALDQKTSPVTAIIFFMLLAAGIGYGIH
                     GLLDDIQSVHETLALGVFALLGIALLIALGFEFVNGFHDTANAVATVIYTNSMAPVAA
                     VIWSGTFNFLGVMVSSGAVAYSIVTLLPVELILQVGNSAGYAMIFALLIAAIVWNLGT
                     WALGIPNSSSHALIGSIMGVGLANQLMAPAGQATSGVDWNQALGVAQALLFSPMMGFV
                     FSALLLVVMKFLVRAPKLYEEPRSKAPPPWWIRGVLIFTCTGVSFAHGGNDGQKGMGL
                     IMLILIGAAPTAYALNRTMPDSATPAFVESTMAAQSVFAAHAAGTAPVAIEDSRHIIG
                     DALRDKNLNRPEVYASLATISADIAGNVRNYGALSHVPAAATPNLRNDMYLAGDAIRL
                     LPKQGAQFTTAENATLKTYSGGLQNGTRFIPLWVKVCVAIALGLGTMVGWKRIVVTVG
                     ERIGKTHLTYAQGASAEMVAALTIGAAEVYGLPVSTTHILSSGVAGTMAANGSGLQWA
                     TVRSIAMAWVLTLPAAMLIAGGLYFILRHVF"
     gene            17064..>17878
                     /locus_tag="VGM72_03120"
     CDS             17064..>17878
                     /locus_tag="VGM72_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264281.1"
                     /translation="MKFSAMARGAAFVIMATLAAAIPHFASAQSNALAGTWNFVPDRS
                     SFMPGPARYKSMTLTYSASGDMTLDGVDAEGKPVKLTYPAVTDGKPHAVSGIDAFDTV
                     SWSKFSDTIATYAFLKRKSNVVLGSRSLSPDGNIMTFNEKTFDTNGKQTGTAVMTFAK
                     PGFETASATAPKAPPPPVVVPTTTPDEDTGTAALGKGDADGAIAAFTLAIDKKDKIAS
                     PVYDHVMRGLAYSEKGMTEQAYADFDAAVMLKPEDKDARFRRGATRVLMKQYDG"
BASE COUNT         3268 a         5671 c         5574 g         3365 t
ORIGIN      
        1 tggtgatggt gccggtcttg tcgaacacca cagccgtcac atcggcaagc tgttcgagga
       61 aggctgagcc cttgatcaga ataccgtgcc gtgccgcgac gacgatcgcg gcaatcgcgg
      121 tcgcgggtgc ggcgagcacc agcgcacacg ggcaggatgc caccagcacc gccagcatcg
      181 ccgggacatt gccggtgacg aaccaggtgc ccgcggcaac cagcagaacc agcgtcatat
      241 attgcccggc atagcggtcg agcagacgcg tgaccggcgg cttggcgttc tccgcttcgc
      301 gcatgagcag gatgatgcgg ccgagcgtgg tgtcttcgcc cacgcgtgtc agctcgatct
      361 gcaacagtcc gtcgatgttc atcgcgccgg cgagaacggt gacgccttca ttcacttcga
      421 ccggcacaga ctcgccggtc agcgaggcca tgtcgaggct ggaggcgccg cggcgcacgg
      481 tgccgtccgc cggcaggcgc tcgcccgcgc gcagttcgat aaggtcgccg gtgcgcaggt
      541 tggcggtcgg caccatttcc accgagccgt ccggcagcag acgccgcgcg tcggtttccg
      601 tcagcttgcc cagtgcctgc accgcttcgc gcgagcccag catgctgcgc tcttccagga
      661 tatgacccac cgtcatgacg atgggcagga acgcggcggt caaaagatcg cccgtcgccc
      721 agcaggcgac aagagcgagc gccaccagac ggtcggtgat gccatgcaag ctgggatggc
      781 gcagggatgc ccaggcttcg gaaaacaccg gcacggccac cattgcggcg gcgacacctg
      841 cgaccaggtc ggaaattccg gtttgcgcag gcaggaacag ccggatcgct ccggccacgg
      901 cgagcaggca gaacgccgcc agcgcaagcg tcaggcgcaa gccgagcagc atgcgctcat
      961 tgccgcccag gagcgaggta tcgtcggagc ttgttccggt cagcggagcg ggaagggctt
     1021 gactgtgatg gctgatgctc atggcgtttt tcccggcaag acaaagcgtg caccgctttg
     1081 cggatcgacc aatgtcacgg accccgtgcg cttcatgatg tccgcgaccc gttcattgta
     1141 agcgcgcagc atgacgctct gccgcgtttg cgcggttgtc tgggccacca gcgccttgat
     1201 ggtcgcggtg tccacggtgg ctgtggtgac acgctcgctg gcggtcgcct ccgcggcgct
     1261 cttcaccctg tcgccttcgc gctcggcccc ttggcggcga agctcggcgg cggtgcgggc
     1321 ggcggcgacc tgctggtcgg cggcctgagt cgcggtcagc acggcgtcga acgcgacctt
     1381 ggcttccggc ggcaaccagg cggtcagatc gatttgcgcg acctctacgc cgagaccgac
     1441 atcctgcgcg ttgagctgct gcaatttcgc attgatccct ttcagcaaat cgtcgcggac
     1501 cgcggtgcgc agcgccacga tcgcctggcc ctgtgcaccg cccgcggtat tgggctgcgc
     1561 caccagaaaa tcgttcaagc cccagccggc cgtcacgttc accgcggtcg cgcggaacag
     1621 acggtcgagc gcgggcgcca catgggcctc ggacaagaca taggcacgcg gatcggaaat
     1681 gcgatagacg aggcgcgcgt ccaggaacac gacattgccg tcgcccgtca gatagggcga
     1741 tgcggtggcg gggatgttgg cggcggcggc ggcgtcctgg gcggagggac gcgcgatccc
     1801 gcccacgggg ggcagggcgc cgacggattt gttgagctgg cggtcgggtc ccggcagcag
     1861 ccgcacttct tcgatgggac gcggccaagc cagcagcagg ccggaattct ggactctcac
     1921 gatgcgctcg aagcgcagca ccacggcctg attgtcgggc gcgatctcgc ggacattgct
     1981 gaacaaccag accacggcga gaagcagcgt caccacatag acggcgcgaa agccaatcgc
     2041 cacggactgg gctatcggac cgggtggcgg ctccgggggc gggccgatat cgcctcggcc
     2101 gatctccccg cgcgcccgct ggatttcgtc ggacagatcg ttcatggggc cgcgcctgcg
     2161 cccgtaccgg gcacgccggt caccagcgcg cggaacggtg ccgcgtcggt cctgagcacc
     2221 atcgcgctgg agcggttcac caccttgctc accgtgtcga gcgaacggac catgttgtag
     2281 agctgcggat cgccgcgata ggcgtcggca tggatcttgg cggcagcctg ttcggcgttg
     2341 gcttcgatct cggcggcttg ttcgcgtgct ttggcgacga cctgccgccc atcgcggtcg
     2401 gcgtcggcgc ggatgatcgc ggcctggcgc aggccttcgg ccgtgcgttc ggcggcgacc
     2461 gtttcacgtt ccgcgcgcat ccgcgcgacg gtggcggcca gcgtttcggt gggcaatgtc
     2521 agacgctcca cgcccacttg gctgatctcg atgccgtaca cctgaagcat gcgctgggcg
     2581 acctgatcgc gcagttgctg ttcgaaggct ggaacgcgca cttttgcggg gtcgatgttc
     2641 accagatcgg cgagatcgaa attgctggcg gtgatgtgaa gctgtgcgct gatgaagctc
     2701 ctgagctgca cggcggcctc atcgggctcg ttgcggaccg aacgcaggaa ttggcggatg
     2761 tgctccggat cggacgacac cttccaggcg gcataggttt gcaccagcac gcgcaagccg
     2821 tccttcgtcc cgacgtcctg caaccccgag gatgtcgtgc gcagccgcag attgatggga
     2881 atcgtcattt cgatggggat cggcaatttc aagcgcaggc ccggctcggt gatcacgcgc
     2941 accggattgc cgaagcgcgt caccaccacg gcctcgcccg ccggcaccat cacgaagcag
     3001 gccgccgaga tcgccgcgat caggacgaac gcggcaaccg cgatgcgcag gcccaacgcg
     3061 atgcgctcgc gcctttcgtg ctcggggcca tgatcgtggt cgtcgtcatg atcgtcatga
     3121 tcgtcggggt gatgatgtga aaacatggcg attatccgat tttgtcagcg ctggctgcgc
     3181 gttgcttgcg aatgaaggcg aacgtcaatt ggcatcctcg acaccgggag tgagaggtct
     3241 tgggccctca ggcgccggag gccgggcggg gggaggcacc gccgcggccg ctggcggcgc
     3301 gcccggcacg gaccccgcaa ccggagcggt cgcgggcgcg gggagcgcgg cgttgccgcc
     3361 ccgcatgtcg atgatgggag cctgcgccgg ggtcagccgg ttgtcgatga tcgtcaaacg
     3421 cacctggctc agcgcggcca cgagggcctg gaacgtgcgt tcggtcacga aggattcctt
     3481 gccctcggca taggccttgc ggtcggcggc gaattgatac gtctcgccct ttgccgactg
     3541 gattttttcc tccgccgagg cttcggccgc actcgtcatc tggtgggctt cttcctgggc
     3601 gacacccgcc atgcgtttgg cgcggccggt ctcgttgaag acgctggcat tggcgttgat
     3661 ctcggcggcc tgcaccgcgt gataggcggc tgcggcgccc accggcggat ggacgctttc
     3721 aatgtgcagg ctgaggattt ccaatcctgc gtgataggcc gcgacttcgc gcgccacatt
     3781 cgcgcgcagg agttcagcca gcatgtcgcg ctgcgcaccc atcaccgtat ccagcgtgtg
     3841 cgaggcgaaa tacatcgaca ccacccggct cgacgcttcg ccgacgagcg tgcccggatc
     3901 ggccgcgcca tagacggcag ccatcgcgtc ggcgtcgctc aggccggtgc gatagacgac
     3961 atggatttcg gcatccacca cctggaagcc ctgcacaccg gcgccgctct ggctcgccac
     4021 cagatattcg gcttccgccg gatcggcggt gtcccacaga tgattggtgc tgggcggcgg
     4081 caccgcttcg gcgtcgatcc gcacgagcgt cgcctcaggc acgctgctgc cgactttgac
     4141 ttcatggagc gtgccgtatt ccaccggccg catgatgccg agcggccagg gcagcagaag
     4201 atggatgcca ggccccagca ccgccaccgg cgcgccgaag cgttcataga tgccgcgctg
     4261 gctgatatcg atcagcttga gcccggaaag gccccagcac agaagcgcgg tggcgagcac
     4321 cgcgggcagg gccgccgacg tcagatagga cagcgcccaa ctgcgcgtga agtcgaggcc
     4381 gagatgttcg cggatcagca tgccgggtgc gcgcgggcct ccggtgacca gaccgaggaa
     4441 gatgctgtcc accgcggccc tggcttcgag cggttggggc acgggaagga acagccgcgc
     4501 cagggcgcgt aaagccagct cgatggtcac gatccagggc acgaccgaga gcgcggcgaa
     4561 aatccaatag acccagagtt ggcccgcgcc gcgtccgatt tccaacgcac cggcggcggc
     4621 cagcaaaatc gtcgtcacca gcagaagccg ccgcaacgcg gtcgcttcgg gcatctgcgg
     4681 caccggaaat accgccatca tgcgctcgcc gatcagcgag cagaacgcca gcgcaatggc
     4741 aatggcgccg accaggttgg gatcggccat ggcagagggc agcgtgcgcg ccatgggcca
     4801 cagcagccag gacgcaaaac aggtcagcgc agcgccggtg gcgatgagga acgcctggcc
     4861 ctgccgcgcg gcaatcccag ggccgatctg gcgcaggaca aagcgccggc cggccggttc
     4921 gttcacgatc tcctcgaccg cgaaatggcg ctgacgcgcg gccgccagca attgcgcgcc
     4981 gaacaatccg gtgagcgcca tcagcagcgt ggcaagcacg agtccgcccc cgccgccggc
     5041 gatggcttcc tgggcttgcg tgcgcagaac cgtgaccggc agccacgcct gaacaaggcc
     5101 aagaaccacc acgaatgtga agccgccata cagccagagc aggcgggtga cgaaacgata
     5161 ggcctggctg gacccgaggc ccaagccgcc tatctcatga tccgcctgcg ccatgcatcc
     5221 tccagcaatg ccgtccccgt caggcggcac cttcgtttgg ttatagaatt agcccaattc
     5281 aaggcatgag tgccacggat aaatacgcag ccagacagca gactcctcag atcccgcgcg
     5341 tttgcgcagc tgaacgccga tggcctataa acgccttgtt cgcacaaggc aaaatgcatt
     5401 cttcatgatc ggccgcggct atcgtttgtg ggttgtgctt ggcctcctga ttgctgccgg
     5461 cggggcggtg ttcgcgtggc ggcagacgca gacaaaccgg ctggaaaccc aggtgctgac
     5521 gctgcttccc gatgaggtgc cgcggcatgc cgatctggtg aatctttcca tcgccgaggc
     5581 caagccggtc tatgccgccc attgcgcgtc ctgccacggc gcggacatga aaggcgatcc
     5641 ccggatcggc gcgcccaacc tcaccgatgg ggtatggctc tacggtagcg ggaatgtgac
     5701 cgatatcgag cgcaccatcc tttacggcat ccgctccggc catgggcagt cgcacaatgt
     5761 caccgagatg accgccttcg gcctgagcgg gcagcttact ccgggcgaaa tcaacgatgt
     5821 cgtgcaatat gtgctgtcgc tgagcaagcg gccgaacacg gcgcaggcgg cactctccgg
     5881 cagccagatt ttcgccggca agggcaattg cggcgattgc cacggacccg atgggcgcgg
     5941 cgatccctat tacggttcgt ccaatctgac cgtcaatgtg tggaacaatg gcggcgacac
     6001 ggcctcgctc tatcgctcga tctatttcgg gctgcagcgg gtttgcccgg cctggtacgg
     6061 aatcatcccg ctcgagaaga tccgcgcgct cgcggtgtat ctgcacgcgg tctctcattc
     6121 cgaagccggg gccgaatccg gggccgcatc cggcgccgcc gctccgccgg caagctgacg
     6181 ctgttcatgg aaagatcctc cgcatggaca tgaaaactcc ccgtcctccg tcgctgccgt
     6241 cgcggctttt cggcggattg cgctatcgtc cgccgcgcgg tgtggcggtg ccccttgccg
     6301 gcgctttcgc cggtctcgcg attgcgttgt tcaatctgct tggccccggt tcgaggccgg
     6361 tctcggtggt gcccgccggt tacgccgcgc tggtcaatca gcgtcccatc ctgatgagcg
     6421 atttcatcaa ccagaccgag accatcgaga gcacgccctt cgccgaaacc acgcctgagc
     6481 agcggcgcgc ggtgttgcat tcgatgatcg acgaggaact gatggtgcag cgcgcgctgg
     6541 tgctcgacct tcccgaacag gacaccgacg tgcgcaagga attggtggac gcggtgggcg
     6601 cggtgatcct ggcgagaaag acgccgaccg acgccgacct catggcctat ttcaatgccc
     6661 atcgcagcaa ttatgcgggc gtgggcgaaa tgcgcttcca cgacatcgtg ctgcatttcg
     6721 gcggctctca ggacgccgac caaagcctgg aacaggccga ggccgatgcg catgaagcgg
     6781 tgtatcagct gcgttcgggc gcgccgctcg aatcggtgat gcagcatttc ggcttcgtgg
     6841 attcgggcaa gaccgacagc accatggacg atctcgattt cgcggtgaag ctgcatctcg
     6901 gcgagaagct gttcaacgtc gccgccaagc tgaccgacgg cgaggtgtcc gaaccggtgg
     6961 acgaagtcga cggcgtccat gtgcttgtca tggaagagcg caagccgccc cgtccggccg
     7021 aattcgccgt cagccgcaat caggtctaca ccgattaccg gcgcgatcag caattgcgcg
     7081 cggagcagga caatctgaaa ttcctgcgcg ccaatgcgca gatccttctc gcgcccggtc
     7141 aaagccagtg aggcaaagcg gatgaatcgc ctctttgttc tcctgtgtct gctgctgctt
     7201 gcggctgggc ccgcaggggc cgaaacgcgc agcgcgtcct atggactgtg gaacgtgacg
     7261 ggcggcacgg tgcgcatcct ctacacgctg ccggacgcgg aggcgaaaag actggccgca
     7321 cccggagccc ctgtcctcac caccaaacag gtggccgatt acatcctggc gcatatgggg
     7381 gtgagccatc ggggcaaggc ctgtcccatt gtcgatcagg gcgaggatct cggcctcatc
     7441 aatacattgg cgctgacgcc gggtttcctg cgtttcgaga tggtcttcca atgtccggac
     7501 ggcgccggcc tcacattgtc cgacacggca tttgccgacc gcatcgccaa tcatgccgat
     7561 tacggccgtg tacagatcaa caacgggggc ctggtccagc atgtctttac cgctgctgcc
     7621 ccgcgcttcg ctttgcccgc gccggggggc acgctgcaag gcgattccat ttttcgttac
     7681 gcgcagctcg gctttgccca tgtcgcgaac ggtgtggaca ttctctgctt cacgctggcg
     7741 cttctcctga tcgcccggcg ccggcgcgat ttcgcgctcg ccttcggcgc gctggccgcc
     7801 ggctatggca cggccgcgct gctcggcagc ttcaatctcg ccgccgtgcg ttttacggac
     7861 ggcgaggcgt tcagcgggat gctggtcttg gccgccgccg cggccggact cgcgttgagc
     7921 cggcccgatc cgcggcgtgc cgcgttgggc ctgtcttcgg gcgcgttggt gcttggcgcg
     7981 gcggcgtggt tctttcacgg tgccgcggcg gcattcgccg ttttggggat cgcgctcctg
     8041 gccatcgcca cgctgtacgt gccggcgcgc gatgaacggc gcgctttgtt cctggtgctg
     8101 gcgtcgtttc tgttcgcgct gctggatggc tttacactgg ccggcgatgt cgcgatcctg
     8161 tttctttcgc cggcacaatt ggcgcccatg ctggtgggct tcaacgccgc tacgctcgca
     8221 ggctcgctcg tgctgcctct ctgtgcgatg gccgcgtggt tgctggtgcc gctgttccgc
     8281 cgcctgacgg tgccgcgtgg tctcgccgcc gaactggcgg catcggtctt catcggcttc
     8341 ggaatcttcg ggttcgcgac ttggctctat agctgagcgt cattcctggg ataagttgca
     8401 gccagtgcgc gtcgaagaaa agaggagccc cccaatgatc cgcagaatcc tgcgaaccag
     8461 catgtccgtc cgcatcgccg tcctggtgtg cggtcttgct ccactcctca tcgcgcgcga
     8521 actcaacgcg caggcggaac tcacagtccc cgcgggactt cccgattgga cgttcaacat
     8581 ccccgacaag gcccagccga gtgccgtgcg ggtggaaggc atcgttaaag cccccggcag
     8641 caccaaggaa tatgaggccg caaagatcgc cggaaacgcg aacccgccgg attggtttcc
     8701 cgatgaacat cctgccgcgc cgcatgtggt cacgggcggc cagggggtca ggtttgcgtg
     8761 cggctcatgt catctcatgt ccggccaggg tcattcggaa gccgccgacc tcgccggcca
     8821 gccggccgag tacctgatcc ggcaaatggc ctattacaaa accggcgcgc gcaaggaaga
     8881 cgcgcgcatg ggtccgatcg cgaagatcac atcggacgag gatgttcggc aggccgcaga
     8941 ctatttcgcc gctttgaagc cgcgcgtctg ggtgaaggtt atcgagaccg ccaccccgcc
     9001 gaagacgttc attgccaccg ccggacggca tcgtcagctt cttcccggcg gcggcaccga
     9061 gccgatcgga caccgtatcc ttgaaatccc cgccgatcca ttcctcacgg agatccggga
     9121 cccgcattcc ggtttcatcg cctatgtgcc gcccggcagc atcgccagag gcgaagcatt
     9181 ggtcaaagac ggagcgtccg gaaagacggt gcaatgcgcg cgctgtcacg gcgaggcgct
     9241 gaagggcaag ggcgacgttc cgcggctcgc ggggttgcaa ccgctctatg tcgcgcggca
     9301 gctcttcgat atgcggtatg ggtcgagcgc gggtgaaacc gcagcgccga tgaagccggt
     9361 cgtcgccaat ctgtcggaag acgacatcat cgcgatttcc tcctatctcg gctcactgcc
     9421 tccgcaatga tcgtgccgcc ggccggcgcc gccgctccat tcctgcgtcg cgggagcgtc
     9481 cgcgattcgc gccggacttc gtcattctaa ccgccagccc ccgcgcgcgg atcttctatc
     9541 acgctgtctt cgggctcgcc gttttcgatt tttgctggat atgcgcgccg tatcgccgcg
     9601 cttgagagtc ccatccggca aaggttggga agaatcaatg taaatccaat ttacgtcaca
     9661 aaggcgaagc cgttttgtcg aaaaacgttc acagcccacg gaaatattcg ttcgcacgaa
     9721 tgaattcgta cgccggggct ttgtgaaaat cagtcgcgcc taaagacgaa ttctcaacat
     9781 tcatcctgag cgaacggcgg atcgcatccg ccgcaatgga gatattatgt caagtgtaag
     9841 acattcctgg attcgttcga tttttctcgg cggcacggcg gtttccgtgg gcatgttgat
     9901 ttgtgcgaac agagcgtcgg cacagcctgc cccgcaggca cagccggtgc aggtggcgca
     9961 ggacgccgtg cccgaaaccg tcatcgtgac aggcacattg ttcgaccccg acgtcgcgcc
    10021 ggccaaagcg tcgttggata cgaaagaacc tcagacgatc atcaatcgtt cctatatcga
    10081 agattcggta tcgccgacgg cggactacgt caccattttg gccattacgc cctcgctcac
    10141 gggcgccgat atcaacggtc ccggtctgtc ggacggcaat gttaaaaaca cgctgcgcgg
    10201 tttgccggat ggtcaatacg gcatgacgta tgacggcata ccgtttggcg acacaaacgg
    10261 gcctactcat cattccgcat cgtattttcc cgccaccaca atcggctcca tcgaagtgga
    10321 gcgcggtccc ggcaatgccg gcaacctggg tccggccacc tttggcggct cgataaacct
    10381 gtattccgag cctctcgccg acggtatgcg cgccaaagga tccgtcagtt tcggcagctt
    10441 cggtacgacg gatcagaacc tcaatttcca gagcggcaat atcaatatcg gcgatatctc
    10501 ttcgacgcgc atcctgttca atttccagaa caccgacagc gatggcgctc tgactcttca
    10561 aaggctcagg cgcaacaacg atcttctcaa gatcgagaac aatctttccg ccaattggac
    10621 gctgacgttt ttcgccaact acagcaagct caccgaaaat ctggatgaca ataatggtgc
    10681 gacgctggcg caggtcgcga cctatggcaa ggatttcgcg ctccagagca gcaatccgaa
    10741 tctgcccacc tattaccagt acaacgtgac caacaaatcg acggatctgg attacatccg
    10801 cctcaagggc aatatcgccg atgacttcaa ggtcgatgag acggtttata cctatgccta
    10861 tgtgaacaag acgatgtcgc cgcgcagcat cctgcagacc ataacggaca tcaacaacgg
    10921 caccgcccag ggcatcggcg gaaaattcaa cccgatcgtg ggcggcgccg tccagacgaa
    10981 cgatatccca ggctatcaga agctcaatgc ttatcgcgtc tggggcaaca tcatccgggg
    11041 gtccgccgac taccaaatcg gcgatatcag cggacaggtc cgcctgggcg tctggtggga
    11101 aggtgcggcc acacagcgcg cgcgcttcga tttcgattcg actttgtgtc tcgcgaacgg
    11161 catcaacccg tgggtcacgt ttggaacctc atgccaggat tcctccctaa cgcccaaaag
    11221 cgcagtgaag gtgaccggcc agggcaatcc cacctacaac ggatatgctg aattgctcga
    11281 acatagcggc tggcagcaat atcagccctt tcttgaagtg gattttcgcc cgatcgaggc
    11341 gctgaccatc acgccgggcg tgaaatatgt cgattggaat cacaccacgg attcggtcgt
    11401 cgaaccgaaa ttgctgaagc cgttcatcgg ctcgttccgg acgacgcgaa ctctgccgtt
    11461 cctggaagcc aattacaaat tcacgaactc ctggagcgtg tacgctcaat atgcgcaagg
    11521 gatttacgtc cccgacatca ccgcattcga gcagaaaacc gcggtggatg ccttcccgaa
    11581 agcgcaaacc acgacgaatt accaggtcgg taccgtgttc tatgccgaca atttcacctt
    11641 cgatgccgat ctctattaca tcccggtgaa gaacaatatc gtgtttgaga attgctctct
    11701 ggccggaggg ttggccggcg acacttgcgg tgtgaacacg ggggaggctc tctacaaagg
    11761 catcgaaggc gaaaccactt atgccttcaa cgatgatatc atgagtggcg ccctgcgcgg
    11821 gctcgtggcg ttcatcaacg ggtcggtgaa ctccgccaag tctcagggct tttacctcaa
    11881 acaggcgccg atgtggaccg cggcagccgg acttgtctac aagcgcgatg ctttcaagat
    11941 atcgctgatc gacaaattgg tcggaccgca atattccgac aacgctcaaa acgtgaacta
    12001 caaagttcac gcctataata atatggacgc tactgtcggc tatgacttcg gcaggttcga
    12061 agcatctgtc ggcgtttata atgttcttgg cagtcgtagc gtccttgcga tcaccgagaa
    12121 cgatgcagcc tttatcgcgg acagacttct cagcaccgac caatattatt tccaggcacc
    12181 tcgcagcttc atggtcacct tgaaagcatc ggtgtcgccc tgatcccggc ggcagcggat
    12241 gctcttctca atcggttacg ccgcagctgt gggaaaagag gtcgtccagc aacgccgcca
    12301 aggcgcggac gttcggttcg gccgcaattc ccacagcgtg acgcttcgcc agccacgcgg
    12361 ggtcgttata agacagccac accttgccgg atgcgtcctg ccagaccaac gccttcagcg
    12421 gcaaatcgat gccggctgtc tgattggatt gcatcagagg cgtgccgccc tcggccttcc
    12481 cgaagatcag aagctcagtc ggcctcagcg aaagaccggc tttcgcggcg cccgaggcat
    12541 gatcgatgcg agcgaacacg ccgatgccct tggctttgat cccggcttca agccggtcca
    12601 ttgtctcggc ggctgtgaaa ctgctgggaa tggtgatcag gccttccatc gccatcggcg
    12661 ctgaccgtta aagatcggac ggcggggcat ctgcgccagc acgcggggcc ggaacggacg
    12721 ttgcgttcgc tggcgtcgtc ttgggcgcgg cgggtgcggt gacggtcaca tcgacctcat
    12781 cccaattggg tccgtccttc gcgttggcga cgcgcacttt cacggtgccg ggcttggagc
    12841 cgatcgtgag ctcgcccgaa ctcgcgtcga tcttgcaccc gagcgcattg ccccgaatcg
    12901 agaaatgggc gtcgcggctg gaggggaaaa gatccgcgct gatctggatc gtattggcgc
    12961 ttcgccccgc tgccgaggtc tccacggccg cgtgatccgc cttcgcatgg gtgacgcccg
    13021 tcagcacgat cttcggatcg ccgtcatagg ccatcacgtt ttccttgccg ttcaccttgg
    13081 cgacgaattc cgcgttcttg ccggatttgt ccaggcgcaa ggtccgctca tgggtggtgg
    13141 tggagttcgc ggaattccaa cggcttccgg ccgcgccatg cggacaccac cagtggaatt
    13201 cctgatccgc tgtgaaccca accggcatgg gattggccgg cgtgggattg gaatcgctcg
    13261 ccagatggcg cccgacatcg atcagcgatt tctggatgcc gaccgtatcg tgattgtccc
    13321 cgagttcgcc gctgtcattc atgaaccagt tgcccttggc cggaatatcg ggaagcgtgc
    13381 cggtttgcag cgtgaactcg ccgaacggcg tcgtcatctt gtgggtcgcg gcttcgggcg
    13441 ttgcgagctt gggaaagcgt tcacccatta ccacctgatc cagcgatgtc acctgcccgt
    13501 cgttgctttt gaagacatga tcgaacaccc cgccaaacag cgagctgtcg gtggggtcgc
    13561 cgatgacatt cgtgctcgcg atgccggtgg gatgggaggc gagacgatag ggcatgacct
    13621 ggccgccttc attgttctcc gccttgatgt cgagcacgcc gcctttttgc gcggccccga
    13681 gtgagagcgc cgcggtgagc gtatcggcgc ccggcgtcaa tgccgttcca gccgcgggcg
    13741 atccggcatc gatcgtccat gtcaccggct tggcggtcgc ggggccggcg gtgaattgga
    13801 tcgccggctg atttccgcat ggcggcacat gcgcgatgct ccgcccgttg ggtccgatat
    13861 tggccttgat ggtgccgacc gcaacctgag tcatcggcac cgcgccctgt gcgggatcgg
    13921 tttgcggcgc gatgacttcc aatgacggat tcgcgggcgg caaatcgggg ccttgatcgg
    13981 tcgcgggcga cggttgcttg ctgccgcctt cgcccgggcc ggagccgcgc gccatctgct
    14041 gttggatcgg cacccggctg aagtcggcgg caaatggcga agcgtcggag cgcgattgca
    14101 aaggagcgtt ttggcgggag gacgctttgc tgcgccaact cttgttcggc gcacaggatt
    14161 tctcctgaag gggaacaaaa tcttgcatga caattccagt cgtgtcgcca gaaaaactag
    14221 catgccgccc gcatgtgcga aaccggacga aaatggagtc aataccaatc gggttcgtcg
    14281 aatacgacat ccatgttgca ggagagcgtt cgcctctccc cctctccgaa gaagggctcc
    14341 acccggtgca acagccaatt ggggaaaaca atgagccggc ccgtttcggg cttgatgtat
    14401 tgctgcgttg gaggccgcag gagactgaaa tcgcgcattc cgtttccacc ccagacgaat
    14461 tgcgtaaacc cgtcgcaatt gccgctcgtc aggttcaact tcggcgaccc gccatgctga
    14521 accgcgggtt tttccgcgat gcccgatggc accttcaaat agagaatgga agacagaccg
    14581 agaggcgtgt tgccgccatg atcgtgcagc ggattgtagt cccccgcata actgtggacg
    14641 ctccacatgt catgggccgt gacggccgcg cgatatccct gctgcgcgac atactgtccc
    14701 gcgatttcga caataacggc cgccagggtt gccggcgctt tctgccgcaa atccaattga
    14761 agctgcgcgg atcgcgcgtg ctgacgaatc tgcccgacga gcgaaggcga atgatcggcc
    14821 gcagaatccc gcacgccatc gacatggtcg ttgatggtgg tcacgagcga ggcaggcaga
    14881 tgcgcttcca tcaaataaag cgcaggtttg atcaccagct cgcaggtgat gtcctcggtt
    14941 tccaattccg tcacgtcgcc ctatcccgac agacagcatt gcacaggcga atgagaacgc
    15001 ttttcctgac gcggacctcg ctcaataaat aacgcaaaat aacgcagggc catcggcgcc
    15061 ctttgcgaat tgtgttgccg cctgcccgcc ctgtaaaggc ctttgctgtg gacatcaggg
    15121 gggcgtgccg aatcttctcg gcacattcat gaaactgtcg cagccaggcg attgaatccc
    15181 ccctgagata aggggcatcg acaatggctg acatagcggc atctcccgaa acattccgcg
    15241 gacgcggcac accggcgctg gaccaaaaaa ccagtcccgt gacggcgatc atattcttca
    15301 tgctcctggc ggccggcatc ggttatggca tccacggcct cttggacgac atccagagcg
    15361 tgcacgagac cctggcgctc ggcgtctttg cgcttctcgg aatcgccctc ctgatcgcgc
    15421 tcgggtttga attcgtgaac ggcttccacg atacggcgaa cgcggtggcg acggtgatct
    15481 acaccaactc catggccccg gtggctgccg tcatctggtc ggggaccttt aatttcctcg
    15541 gcgtcatggt gtccagcggc gcggtcgcct acagcatcgt cacgcttctg ccggtcgagc
    15601 ttatcctgca ggtcggcaac agcgcgggct atgcgatgat cttcgcgctg ctgatcgcgg
    15661 cgattgtatg gaatctcgga acctgggcgc ttggcatccc caacagttcc agccacgcgc
    15721 tgatcggttc gatcatgggt gtcggccttg ccaaccagtt gatggccccg gcggggcagg
    15781 cgacctcggg cgtggattgg aaccaggccc tgggcgtcgc tcaggcgctt ctgttcagcc
    15841 cgatgatggg gttcgtgttt tcggcattgc tgctggtggt gatgaaattc ctcgtgcggg
    15901 cgccgaagct ctatgaagaa ccgcgcagca aagcgccgcc gccgtggtgg attcgcggcg
    15961 tcctgatctt cacctgcacc ggggtttcgt ttgcccatgg cggcaatgac ggccagaagg
    16021 ggatgggcct catcatgctg atcctgatcg gcgcggcgcc cacagcctat gcgctcaatc
    16081 gcaccatgcc cgattccgcg acgccggcgt tcgtcgaatc gacgatggcc gcccaaagcg
    16141 tgttcgcggc ccatgccgcg ggcacggccc ccgtggcgat cgaggattcg cggcatatca
    16201 tcggcgatgc gttgcgggac aaaaacctca accggccgga ggtctatgcg agtctcgcga
    16261 cgatctccgc ggacattgcc ggcaatgtcc gcaattatgg ggcgctgtcc cacgttccag
    16321 cggcagccac gcccaatctt cgcaacgaca tgtatctggc cggcgacgcc atccgtctgc
    16381 ttcccaagca aggcgcacaa ttcaccaccg ccgaaaacgc aacgctcaag acttattccg
    16441 gcgggctcca gaacggcacc cgcttcattc cgctgtgggt caaggtgtgc gtcgccatcg
    16501 cgcttggcct cggcaccatg gtgggctgga aacggatcgt ggtcacggtc ggcgagcgga
    16561 tcggaaagac tcatctgacc tatgcgcagg gcgcttcggc ggaaatggtc gccgctctga
    16621 ccatcggcgc ggcggaggtc tatgggcttc ccgtgtcgac cacgcacatc ctctccagcg
    16681 gggtcgccgg cacgatggcg gccaacggct ccgggttgca gtgggccacc gtgcgctcca
    16741 tcgcgatggc atgggtgctc acgcttcccg cagcgatgct gatcgccgga ggactgtatt
    16801 tcatcctgcg ccatgtcttc tgaggttcga acaaccgcag aagaacgagg ccgctaacgc
    16861 cgcagccatc cagacaacgc atggtttcct tagcgttgtg ccggatgcgc tttgctcgcg
    16921 acaatgtcag gattcgtgcc ttgccctcgt gagaagggca attctatact tcggcttgtg
    16981 cttgccgtct ggcggctgga cgcgttgttg cgcgcccatt gcggttatgt gtccactaaa
    17041 tgtcgaggaa tggggaaggt ccgatgaagt tttctgcaat ggcgcggggc gccgccttcg
    17101 taatcatggc cacactcgcc gctgcgattc cccatttcgc gtccgcgcaa agcaatgcgc
    17161 tcgccgggac ttggaatttc gttcccgacc ggtcgagctt catgccgggc ccggcccgct
    17221 ataaaagcat gacgctgacc tactcggcgt ccggcgatat gacgcttgat ggagtcgacg
    17281 cggaaggcaa gcccgtcaaa ctcacctatc cggccgttac cgacggcaaa ccgcacgccg
    17341 tcagcggcat cgacgccttc gacaccgtgt cctggagcaa gttcagcgac accatcgcca
    17401 cctatgcgtt cttgaagcgc aaatcgaacg tcgtcctcgg cagccgctca ctgtctcccg
    17461 acggcaatat catgacgttc aacgagaaga cgttcgacac caacggaaag cagaccggca
    17521 cggcggtcat gaccttcgcc aagcccggtt tcgagacggc aagtgccacg gccccaaagg
    17581 ccccgccgcc gccggtcgtg gtgccgacca ccacgcctga cgaggacacc ggcaccgccg
    17641 cgctggggaa aggcgacgca gacggtgcga ttgccgcgtt caccctcgcc atcgacaaga
    17701 aggacaagat cgcctcgccc gtctacgacc acgtcatgcg cggccttgcc tactccgaaa
    17761 agggcatgac cgaacaggca tacgccgatt tcgacgccgc cgtgatgctg aagcctgaag
    17821 acaaggatgc ccgtttccgc cgcggcgcca cgcgggttct gatgaagcaa tatgatgg
//
LOCUS       DASYEG010000084         3526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_5100056, whole genome shotgun sequence.
ACCESSION   DASYEG010000084 DASYEG010000000
VERSION     DASYEG010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5100056"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="VGM72_03125"
     CDS             complement(<1..1169)
                     /locus_tag="VGM72_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY4264282.1"
                     /translation="MIDLPAPNPVTLSRRAEIADALKAIVPGGVIGDEATLAAFDCDA
                     LTAYRQSPLLCVLPENAEQVSNVLAYAARENIRIVPRGAGTSLSGGALPLADSILLGL
                     SRMRRILEIDYDNRCIVVQPGVTNASVTRAVQDEGFYYAPDPSSQIACTIGGNVAENS
                     GGIHCLKYGLTTHNLMGIELVLMNGERLRLGGKHAGDGGLDLLSVVCGSEGLLGVVTE
                     ATLRILPRPPVTKTVLIAFDTVRAAGETVAAVIAAGIVPAALEFMDRRCIDAVEAFCA
                     PNYPRDAEAILIGEIDGMPAEVAGDARRMGEIAIACGATSVRDCADEADRARVWSGRK
                     AAFGAMGRLEPDMLCMDGTIPRKALPEMLDRICALSDHYGLVCCNVFHAGDGNLHP"
     gene            1326..2987
                     /locus_tag="VGM72_03130"
     CDS             1326..2987
                     /locus_tag="VGM72_03130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEY4264283.1"
                     /translation="MWSQIYDPLGNAVLSTIAAAIPTILLLALIASGKMKIHLAAVVA
                     VIVAAAIAITIYGMPADLAVRAIGLGALAGFFPIGWIIVNVIFLYRLQVEAGAFAIIQ
                     QSIGAITEDRRLQLLLIAFALGAFFEGSSGFGTPVAMTGSILMSLGFSPLAAAGLSLI
                     ANTAPVAFGALGAPVTGLQQATQLDFNMLGAMIGRQLTLFSVIVPFWLIWAFAGFRAM
                     KEIWPAAFICGLTFAVGQFAISNYISPALASIGAAVICLFCLRGFLYVWQPKTIWTSA
                     ALRSADHSHSTMPPLKPMGAKASSSQVQKAWLPLILLCIILVTWGMPPVKAFLDNIFS
                     PRFDIGGLHNMVLRMPPVAPMPTPEPAFFRFFFLSYSGTAILVTAIISGFIMGFSPKR
                     MVQVYIETFGVVRYSIITISAMVALATLTRYAGVDATLGLAFAASGVLYPFFGTLLGW
                     LGVATTGSDTASNLLFGNLQRITSEQLGLSPILMAAANSSGGVMGKMIDAQSIVVAST
                     ATGWVGHEGTILRFVFVHSIVLAVLVGLLVTLQAYVYPFTEMVVR"
     gene            3090..3314
                     /locus_tag="VGM72_03135"
     CDS             3090..3314
                     /locus_tag="VGM72_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264284.1"
                     /translation="MSTLSPALDLFLYQFLRFFNPRQGYCWIYLAGGLFFALCVMLWR
                     RRNRSRITPARFCGFSARARFGCTAPRFWT"
     gene            3311..>3526
                     /locus_tag="VGM72_03140"
     CDS             3311..>3526
                     /locus_tag="VGM72_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264285.1"
                     /translation="MKLYFLHGVLVLIAYGMFEATSEVWRASTVSALAALAGAEPRLH
                     APHAIVGSLTTMLQIIALDLGYLALHYC"
BASE COUNT          577 a         1116 c         1087 g          746 t
ORIGIN      
        1 ggatgcagat tgccgtctcc ggcatggaag acgttgcagc agacgagccc ataatgatcc
       61 gacaatgcgc aaatccggtc cagcatttcg ggcaaggctt tcctcgggat cgtgccgtcc
      121 atgcagagca tgtcgggctc cagccggccc atcgcgccaa atgccgcctt gcgtcccgac
      181 cagacgcgcg cgcggtcggc ctcatcggcg caatcgcgca cgctggtggc cccgcaggca
      241 atcgcgattt cgcccatgcg gcgcgcatcg ccggcgactt ccgccggcat gccatcgatc
      301 tcgccgatca ggatggcttc ggcatcgcgg ggataattcg gcgcgcagaa cgcctccacc
      361 gcgtcgatac agcgccggtc catgaattcg agcgcggccg gaacgattcc ggcggcgatg
      421 acagcggcca ccgtctcgcc cgccgcccgc accgtgtcga acgcgatcag cacggtcttc
      481 gtcaccggcg gacgcggcag gatccgcaag gtcgcttccg tgaccacgcc gagcagccct
      541 tcggagccgc acaccacgct caaaagatcg agcccgccat cgccggcgtg cttgccgccc
      601 agacgcaagc gttcgccgtt catcagcacc agttcgatgc ccatcagatt gtgagtcgtc
      661 aggccatatt tcaggcaatg aataccgccc gaattctccg ccacattgcc gcctatggtg
      721 caggcgatct ggcttgaagg atcgggggca taatagaacc cctcatcctg cacggcgcgc
      781 gtcaccgagg cattggtgac acccggctgc accacgatgc agcgattgtc ataatcgatc
      841 tccaggatcc ggcgcatgcg ggagagcccg agcaggatcg aatccgccaa gggcagcgct
      901 cctccggaga gcgacgttcc ggcgccgcgc ggcacgatgc ggatattctc gcgcgccgca
      961 taggccagca cgttcgagac ctgctcggcg ttttccggca agacgcacag aagcgggctc
     1021 tggcgatagg cggtcagggc atcgcaatcg aaggcggcca gcgtcgcctc atcgccaatc
     1081 acgccgccgg gtacgattgc cttcagggcg tccgcgattt cggcgcggcg cgacagggtc
     1141 acggggtttg gggcgggcag gtcgatcata ggccgggtct attaccacaa atagagcgcc
     1201 gcaccccctt tacggggcct cggcaaatgc caacactcaa aaaacatagc gcagtccgcc
     1261 ggggtgattg ccggttcggc gtaagattgc gcggccaggg gataagggga gctgggggca
     1321 ggggcatgtg gagccagatt tacgacccat tggggaatgc ggtgctttca accattgccg
     1381 cagcgattcc aacgatttta ttgcttgccc ttatcgcctc gggaaagatg aaaatccatc
     1441 tcgcggcggt ggtggcggtg atcgtggccg cggcaatcgc gataacgatc tacggaatgc
     1501 cggccgacct cgctgtgcgt gcgatcgggc tcggcgcgct ggccgggttt ttcccgatcg
     1561 ggtggatcat cgtgaacgtc atctttctct accggctgca ggtggaagca ggcgcattcg
     1621 ccatcattca gcaatcgatc ggcgccatca ccgaggaccg gcggctccaa cttttgctca
     1681 tcgcctttgc gctgggtgcc tttttcgaag gatcgtcggg cttcggaacg ccggtcgcga
     1741 tgaccggttc gatcctcatg agcctcggct tctcgccgct tgccgcggcc ggtttatcgc
     1801 tgatcgccaa caccgcgccg gtcgccttcg gggcgctggg cgcgcccgtc acgggcctgc
     1861 aacaggcgac ccaactcgat ttcaacatgc tgggcgccat gatcggccgc caattgacgc
     1921 tgttctcagt cattgtgccg ttctggctga tctgggcatt cgcgggtttc cgggccatga
     1981 aggaaatctg gccggcggcg ttcatctgcg gtctcacctt cgccgtcggg caattcgcga
     2041 tctcgaacta tatcagcccc gcactcgctt cgatcggcgc ggcggtgatt tgtctcttct
     2101 gcctgcgcgg ctttctttat gtctggcagc ccaagacgat ctggacgtcg gcggcgctca
     2161 ggagtgccga ccattcccat tcgaccatgc cgccgctaaa gccgatgggg gcgaaggcca
     2221 gttccagcca agtgcagaaa gcgtggctgc ccctgattct tctttgcatc atcctggtga
     2281 cctggggcat gccgccggtg aaggcgtttc tcgataatat cttctcgccg cgattcgata
     2341 tcggcggact gcacaatatg gtgctgagaa tgccgcccgt ggcgcccatg ccgacgcccg
     2401 agcccgcttt tttccgattt ttctttctct cttatagcgg cacggccatt ctggtgaccg
     2461 ccatcatttc cggattcatc atgggctttt cgcccaagcg catggtgcag gtctatatcg
     2521 agaccttcgg agtagtgaga tattccatca tcacgatttc ggccatggtg gcgctggcca
     2581 cgctcacgcg ctatgcgggc gtggacgcga cgctcggcct cgcctttgcc gcgagcggcg
     2641 tgctgtatcc cttcttcggc actttgctcg gctggctcgg cgtggcgacc acggggtccg
     2701 acaccgcctc caatctgctg ttcgggaatc tgcagcgcat cacgtcggaa cagcttggcc
     2761 tctcgcccat cctgatggcg gcggccaatt cctcgggcgg cgtgatgggc aagatgatcg
     2821 atgcccaatc catcgtcgtg gcctccacgg caacaggatg ggttggccac gaaggcacca
     2881 tcctgcgttt cgtgttcgtg cactcgatcg tgctggcggt cctggtcggg cttctggtga
     2941 cgctgcaggc ctatgtctat ccgttcaccg aaatggtggt gcggtaggca ttgcggtttt
     3001 tccatctctg gaacggatgg cggtcgattg gcgggagggg ccaggattaa gttaacggcc
     3061 tggaaagcgt ggcgcggcca gattggcgca tgtccacgct gtcgcccgcg ctcgatctgt
     3121 tcctttatca gtttctgcgt ttcttcaatc cgcgccaggg ctattgctgg atctatcttg
     3181 ccggcggatt attttttgcg ctttgcgtca tgctatggcg gcggcggaat cgttcccgga
     3241 tcacgcccgc ccgtttttgc ggcttctcgg ctcgcgcgcg ctttggctgc accgctccac
     3301 gcttctggac gtgaaattgt atttcctgca cggcgtgctg gtgctgatcg cctatggcat
     3361 gttcgaggcg acgagcgagg tctggcgcgc gagcactgtg tccgctctcg cggcgcttgc
     3421 gggagcggag ccgcgcctgc atgccccgca cgcgatcgtg ggctctctca cgacgatgct
     3481 tcaaatcatc gcgctcgatc tcggttactt ggcgctgcac tattgc
//
LOCUS       DASYEG010000085         3092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4519684, whole genome shotgun sequence.
ACCESSION   DASYEG010000085 DASYEG010000000
VERSION     DASYEG010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4519684"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VGM72_03145"
     CDS             complement(<1..327)
                     /locus_tag="VGM72_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEY4264286.1"
                     /translation="MAASRAMRICFIGDSFVSGAFDSECQGWAGRISAAARARGHDLS
                     HYNLGIRGETSIQIAKRWRAEAEPRQSPQQEGRLVFEFGVNDVREVNGKRQLEAAQSL
                     AAAREIL"
     gene            complement(337..1125)
                     /locus_tag="VGM72_03150"
     CDS             complement(337..1125)
                     /locus_tag="VGM72_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY4264287.1"
                     /translation="MKRIMFAASLSLIMSAASAFAASSGLVPAGILRATFIATNPVQG
                     IVEAKTGAVKGPASDLTRALAERLSVPFKVTPANGVQGVLASVKNGEADIGFLAFDPG
                     RAAEVDYSQIYSLAQNTYMVLDASPLKSVSEIDRAGVTIGVTQGDTGDLYLTRTLKSA
                     AFLRNKDGDMAVAMRLLRGGEIAAYGTNRQRLYELAQANPGFRLLPDNFYSVPQAIIV
                     KKGNSALLATIEPMIDAARRSGLIAASIRNAGLVGVDVAPPPAK"
     gene            1574..2449
                     /locus_tag="VGM72_03155"
     CDS             1574..2449
                     /locus_tag="VGM72_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY4264288.1"
                     /translation="MRVSHLLLALLVCVAWGLNAVAAKVGVTYIPPVFFTALRFVFVL
                     ICLAPWLKPVPGKWNVLIPAVFFMGGLHFALIFTGAKLSDASTMAIVNQLYVPISVLL
                     AMAWLGEAVPQRRWLGIAIAFCGVVVFSLDASVASHLMGVFILFLDAISMAIGTVLLR
                     RLSGVPPLVMQAWMAALGIPFLFLTSLVFETGQFAALAQSPWQGWAALAYTVIAGSLI
                     GHTAYYVLLQHYEVSLVGSVLLLGPAIGVLGGVAILGEPFTPMIVIGAAMTLFGVAIV
                     LRRVPMRRLEPAEGV"
     gene            2536..2862
                     /locus_tag="VGM72_03160"
     CDS             2536..2862
                     /locus_tag="VGM72_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264289.1"
                     /translation="MRAWIFAIAISVAFTPAPAAFAAPTCQDQNGATIKCGIPGAMPV
                     GWRLSVQERLDRQMPAPEYQSANELLKLICVIGIFFALMALMPEFDGRRAGDWDRQED
                     DGEDRR"
BASE COUNT          510 a          942 c          983 g          657 t
ORIGIN      
        1 caggatttca cgcgcggcgg cgagcgattg ggcggcctcc aattgccgtt tgccgttcac
       61 ctcgcgcaca tcgttgacgc cgaattcaaa aacaagccgg ccctcctgct gcggcgactg
      121 acggggctcg gcctctgccc gccagcgttt ggcgatttgg atactggttt cgccgcgaat
      181 gccaagattg taatggctga gatcatgtcc gcgtgcacgc gcggccgctg aaatgcgtcc
      241 cgcccagccc tggcattcgc tgtcaaaggc gccactgacg aaactgtcgc cgatgaaaca
      301 gatgcgcatc gcgcgtgagg ccgccatgat gggctcctat ttcgcgggcg gtggagcgac
      361 gtcaactccg acgaggccgg cgtttctgat cgatgcggcg atcagaccgg agcgtcgtgc
      421 cgcatcgatc atcggttcga tggtcgcgag cagcgcgctg ttgccctttt tcacgatgat
      481 cgcctggggc acgctgtaga aattatccgg caaaagtcga aaacccggat tggcctgggc
      541 caattcatag agccgctgac gattggtgcc gtaggccgcg atctcgcctc cgcgcagcaa
      601 cctcattgca accgccatgt cgccgtcttt gttgcgcagg aatgcggcgc ttttcagcgt
      661 ccgcgtcaaa tagaggtctc ccgtgtcgcc ttgggtgacg ccaatggtga cacccgcccg
      721 gtcgatttcg ctcaccgatt tcagcggcga ggcgtcgagc accatgtagg tgttctgcgc
      781 cagcgaatag atttgcgaat agtccacttc ggccgcgcgg ccaggatcga aggcaaggaa
      841 tccgatgtcg gcttcgccat tcttgacgct cgcgagcaca ccttgcacgc cgtttgccgg
      901 ggtcactttg aagggcacgc tcaggcgctc cgccagcgcg cgtgtcagat cgctggccgg
      961 gcctttcacc gcgccggtct tggcctcgac gatgccctgc accggattgg tcgcgatgaa
     1021 ggtggcgcgc aagatcccgg ccggcacgag gccggacgaa gcggcaaaag cggaagcggc
     1081 gctcatgatg agggacaagc tcgcggcgaa catgatgcgt ttcataaccc acccatttgg
     1141 gatgcgaagc gtgcctttgc ctatcatcga aacctggggc cgcgccagat gcgccagatg
     1201 aggcaaacaa aaaggccgga agcggtgctc ccggcccgat ccctttgcgc gcgcgttttg
     1261 ttagaagacc cccatgacga tggccccgag gaatgaaaaa acgaaaacga cggtcagaag
     1321 ctgcaactcc cgcaccatgg agacactcct tcttcccatt gcggcggtag tgtggcgcat
     1381 cttgagggcc ggctccaaga ggaaatttgt gctgcagcgc cgcaatattt tcatgacgcg
     1441 cgcgtcatcg cggcccgctc atgcaagggc ttgcgcgtta agatctcatc gccgccatat
     1501 cttgcagggc tgtatcgtaa ttggacgggg attggcttaa tgctgccggc gcatttcttg
     1561 tgaatccaat ttcatgcgcg tctcccatct tctgctggcg cttctcgtct gtgtcgcctg
     1621 gggcctcaac gcggtcgcgg cgaaagtggg tgtcacctat attccgccgg tgttcttcac
     1681 ggcgctgcga ttcgtctttg tactgatctg tctcgcgccc tggctgaagc cggttcccgg
     1741 caaatggaat gtgctgatcc cggcggtgtt cttcatgggc ggcctgcatt tcgcgttgat
     1801 ctttaccggc gcgaaattgt cggatgcctc gaccatggcg atcgtgaacc agctttatgt
     1861 gccgatctcg gtcttgctgg cgatggcttg gctcggcgag gcggtgcctc agcggcgatg
     1921 gctcggcatc gccatcgcgt tttgcggcgt ggtggtgttc agcctggatg cgagcgtcgc
     1981 cagccatctg atgggggtgt tcatcctgtt cctcgatgcg atttccatgg ccatcggcac
     2041 ggttctgttg cggcgccttt cgggggtgcc gccgctggtc atgcaggcct ggatggcggc
     2101 gcttggcatc ccgtttctct tcctgaccag cctcgtgttc gagaccggcc agttcgcggc
     2161 tctggcgcaa tcgccgtggc agggctgggc ggcgctcgcc tacacggtca ttgccggaag
     2221 cctgatcggc cacaccgcct attatgtcct gctgcagcat tatgaagtgt cgctggtggg
     2281 ctcggtgctg ctgctgggac ccgcgatcgg tgtgctcggc ggcgttgcga ttctgggcga
     2341 accgttcacg cccatgatcg tgatcggcgc cgcgatgacc ttgttcggtg tcgcgatcgt
     2401 tctgcgccgc gtgcccatgc gcaggctcga gccggccgag ggggtctagg cgaaggccgg
     2461 catggcctca ggctgcggaa tgttctaccg tcccgcttcg ttgggcgggc gttcgttggt
     2521 cgtgcgagga acgtcatgcg cgcttggatt ttcgctatcg ccatttcggt cgcgttcaca
     2581 cctgcgcccg cggcgttcgc ggcccccacc tgccaggacc agaacggcgc caccatcaaa
     2641 tgcggcatcc ccggcgcgat gccggtcggc tggcggttgt ccgttcaaga gcgtctggac
     2701 cggcaaatgc ccgcaccgga ataccagagc gcgaatgaac tgttgaagct gatctgcgtg
     2761 atcggcatct tcttcgcgct gatggcgctg atgcccgaat tcgacggacg gcgcgcgggc
     2821 gactgggacc ggcaggaaga cgatggcgag gaccggcgct gagcaggccc tctctcgaag
     2881 gaacatcttt gctttcttcg cgctcccgcc ccgtgatagt ttgacggcgc gctgaacgtt
     2941 cacacaccgc cacgcacaat taaaaccagc gcgacaggga ggcagccgtg aagataagaa
     3001 tgttcgccgc gttgttgttt gcttcgatgc tgaccgcggg cagtgcgtcc gcgcaaccgg
     3061 ctccgttcaa cgcggacggc gtgaccatgg gt
//
LOCUS       DASYEG010000086        18276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4953575, whole genome shotgun sequence.
ACCESSION   DASYEG010000086 DASYEG010000000
VERSION     DASYEG010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 18276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18276
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4953575"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(330..1331)
                     /locus_tag="VGM72_03165"
     CDS             complement(330..1331)
                     /locus_tag="VGM72_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEY4264290.1"
                     /translation="MKQIPVISLILSIAFSTSAIAAAAPNYKIVDRIKVQDGGFDYAT
                     FDAKNGRILMARPDMTTVIDVKTGKVSELKSAVKGHIALPIPGSALLAVTQRGGDVLL
                     VDGAADKVVATFKGGKNPDGATYDPSSKMIFAMDHEAGNAIVIDPATKKLVTRIPVGG
                     ELEFAVPDGAGKVYVNVEDKNQIAVIDVAGKKVTARYALKGCDGPTGLAYDAADKLLI
                     SSCDGVAKVVRADTGAEVASLPTAKGADAVIFDAARKLAFIPCRAGTLEVLSFADPAH
                     ISIVQHVPTQVGTRTGTLDPATGRIYSMAAKFDPPAVAGGRPQALQASYEVLVIAPQ"
     gene            complement(1759..4863)
                     /locus_tag="VGM72_03170"
     CDS             complement(1759..4863)
                     /locus_tag="VGM72_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY4264291.1"
                     /translation="MNLAQWAAQHRRSILFLLAVVAIGGAFSAFGLPVALFPEVNFPR
                     IAITIDAGDQPAAQMVTQVTRPVEQAVKAVPGLLEVRSTTSRGTAELSLNFDWGIDMA
                     VATLQVQAEIARVLPSLPAGATFDIRRMNTNVFPVAAYSLTSDKLDLVKIREIAQYEL
                     LPLVSSIKGVQRVEILGGAAREIRVDIDPARLASYGLTMDDVSKALTAANVLQAVGRV
                     QDRHKLLLTLTDNQLTTPDQIRQTIVRGGPAGAVYLSNVGRVYETAAPSWTIVTGDGH
                     PAIILQIKQQQDGNTVQIVNDVKAALTAYAPKLPPDFHISNWYDQSELILGSARSVFE
                     AVLIGVALAGLVLLLFLRNLKITLITLIVVPAVLASTVLLLSVLGMSFNIMTLGGMAA
                     AIGLIIDDAIVIIEQIDRRLHSTGERGHAEIRAATWEFLQPLAGSSAATIIIFVPLAF
                     LTGVTGAFFKALSLTLAAALIVSFLAAFFVIPLLADYVLTAKDAKAEEHTGPVFRRVL
                     RYYRKTFKGLLAFPVLALPVFAIFLAAGYLAYTQVGTGFMPIMDEGGFVFDYISPPGT
                     SLEDTNAMLNQVEKILRATPEVLTYSRRTGLQLGAGLTESNTGDIFVRLKPAPRRPIE
                     EVMTEIRQKAQKTIPALDVETAQLMEDLIGDLTAVPQPIEVQIYGDDPAVLNEIAPKV
                     ADAIMGVPGITEIRNGVVIAGDAFDIRVDPLKAGLEGLNPDLVTQQINAYLSGTVATQ
                     VRNVDRTIDVRVWVPPKERATPNDIREFLITAPDGHKVPLRRVAQVSLLTGQPEITRD
                     NLKPMVAVTARIEGRDLGSTAAEVKALLDKSGLFTQGTYFQLGGLYAQQQLAFRGLIG
                     VLIAAIALVFALLIYLYERLTLAILIIAMPLSAMGAVFMGLWLTGIELNISAMMGMTM
                     VVGIVTEIAIFYFSEYQLLIREGMTHERAIIEAGANRFRPIAMTTIAAILALLPLAIG
                     LGQGSDMQKPLAVAIISGLIVQMPLVLLIMPLAFSVLTRKSGTRMAGSDSPSFDGPAR
                     QPAE"
     gene            complement(4860..6023)
                     /locus_tag="VGM72_03175"
     CDS             complement(4860..6023)
                     /locus_tag="VGM72_03175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY4264292.1"
                     /translation="MKHPLLRWILLVGALLVTAAVITIVVFPGLFGINERPAGEAEEE
                     EIQGTATVTLATPTSGSLAPVALAYGTVASSPQNAFVIALMRDGVFKSVNVRDGDAVR
                     AGQPIVTVTTAPANVAAFLQAKQAVDFAMQDLTRVERLFANKLATNDQLATAHKILAD
                     AQVQLDQQRMIGAGETEVTLRAPFDGVITGLMATPGDKVQANTTIATVAKRSDVVIEL
                     NLDPQDASKLAPGAAVRLTVSFGDGSEIAGKLTSIGASIDPMTHLVKAIANVPAANSA
                     HLALGTTLLAHIDLPSVQGIVIPRMALLEDAKGSYVFTVSEGEAKRQDIKVLVETDDM
                     AIIDGLDPALGTQVVISGNSELDDDTPVQVEGQPSAEAAPAAQAAAPVKEAKP"
     gene            complement(6020..7363)
                     /locus_tag="VGM72_03180"
     CDS             complement(6020..7363)
                     /locus_tag="VGM72_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY4264293.1"
                     /translation="MLRRHLSRAAGNLAAVLGAVFALTGCATYSTYAPVPLPETVNLS
                     ERIPAQAAQPLDINAVATVAVLNNPDLKAARLKAGVAEAQAFAAGILPNPVLTANLDP
                     PIGGGSVVTGYGLGIEYDIQALVVQPARLAAANAARDEARLSLLWQEWQTVAQARTLY
                     VQRINAAEKRELFAALEGRYATQAERSGRALQAGDVTFDQAGIDLAALLDARSQLRTA
                     ERIQSQTDYNIRALLGVAPKVDVPLQPLTPPPIPTSAEIDAALAKVAQTRPDLRALQF
                     GYQSQEETFRRSVLAQFPSLSFGLGTANDTTNVHTLSLGATLNLPLFDRNQGDIAVQS
                     ATRAQLLAEYQARLNQATGDAWRLWTEMQQLVEAIRDIDARLPELQMASENGERAYLA
                     GDLPALTYVTLQSSLLNRQSELSDLRQSLWNDAIALSSVLGTQIEPVIETKELMP"
     gene            7534..8211
                     /locus_tag="VGM72_03185"
     CDS             7534..8211
                     /locus_tag="VGM72_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003097513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4264294.1"
                     /translation="MRILVVEDDAETASYLVAGLEEEAFTVRRAANGRDGLLIAMTEA
                     FDLLIVDRMLPQLDGLTLVKTLRTASIQTAVIFLTTMTGIDDRVTGLNAGGDDYLVKP
                     FAFSELVARVRALSRRPRGTAVETRLAVADLELDLLKRVVKRNGNEIQLQPREFRLLE
                     YLMRHAGQVITRTMLLDNVWNYHFDPRTNVVESHLSRLRTKIDKGFSPELIHTVRGAG
                     YVLRAPE"
     gene            8198..9550
                     /locus_tag="VGM72_03190"
     CDS             8198..9550
                     /locus_tag="VGM72_03190"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY4264295.1"
                     /translation="MRRNRLLHASSFRLGLLYAGLLTASVLVLFGVTYWFATDYAARD
                     ETDEIGVEFASIQDEVRSSGEARLPTIIDNHVRLRKDVRAVYLLQDPSGHKIAGNIEA
                     MTPFLGPKILDRIFDGEKRQVRAYGYNLSNGDYLLVGQDTATLRQMKALIFRSFGIGF
                     IVTVLLAILGSVIVSSTVLKRVETVGRTARAIIGGDFSQRVPVRGTDDEFDELAASMN
                     AMLDRIEDLMRSMRQVSNDIAHDLRTPLTRLRQRLEHARRRTHTVEELQEALGHSIAQ
                     VDSILETFGALLRIAQIEADTRSAREVSVDVSKLLSGIVDDFAPAAEDHGQQLVAEIE
                     SRLSAKADPELLTQMVVNLVENAIRYSPVGARIAVQARASAGYLELAVSDTGPGIPMQ
                     ERENVLRPFYRLEASRTTEGSGLGLSLVAAIAKRHQAKLSLNDNDPGLRVVVLFPVAA
                     "
     gene            complement(9981..11738)
                     /locus_tag="VGM72_03195"
     CDS             complement(9981..11738)
                     /locus_tag="VGM72_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264296.1"
                     /translation="MIRRNKALSLKLRLIRKRRSLVVLSGTLGIVTTLTGLAIGYGRA
                     AERGASAHPPVDHMLFTTSLHCIACHSKVFAPNGEDISIGYQWRASIMANSARDPYWQ
                     AGIRRETMDHPQAKAIIEDTCSTCHMPMQRFQARAENQSGEVFRYLEAIRSGMANVEP
                     EAKLEDAAEPKATLAADGVSCTLCHQVRPDNFGQESSLDGGFVIDLTKKPEEREIFGP
                     FDVDKGRTRIMHSVTGFTPTKTEHIKQSELCATCHTLLTRALDDKGNPAGTLPEQVPY
                     QEWQHSDYAQTNSCQSCHMPRVAGDAPITSVHSQTHQEVSRHVFVGGNAFLLRILKDH
                     RKELGVIATADELEASAKRTENQLGTETASLTIKNATVTAGRLEFGVMVTNKAGHKFP
                     TAYPARRAWLHVVVRDSRGALVFESGASRPDGSIAGNDNDEDATKFEPHYTRITSTDQ
                     VQIYESIMGDFANRVTTGLLYGTHYLKDNRLLPRGFDKTNAEPRVMVVGPAREDSDFT
                     GGSDSLTYSAALPNGNAGAPYNVTAELLYQSIGYRWAQNLRNYDAPEPKRFVSYYTQA
                     AGASAILVARSALTTSSSP"
     gene            11917..13044
                     /locus_tag="VGM72_03200"
     CDS             11917..13044
                     /locus_tag="VGM72_03200"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY4264297.1"
                     /translation="MTAIFILRPAAGFIAASSAFLLLAIVAAASQPAPPDGHAIFLAN
                     CATCHGVDGRGMRTPAEVGFDLPMPNFSDCSFATREADADWSSTIHRGGRQRALARIM
                     PAFEDALSDDEIDAVIGYLRTFCTDPRWVRGEFNFPLGLFTEKAFLEDELVWTTAIDA
                     KSPNNIQSQFILEKRIGATGQLEFTLPFARMDGGPGVGRNFGVGDFGVAWKQNVVADV
                     DSGTIFSVLGEMVLPTGSEKKQLGTGSLSFETHALFAQMLPDDFVYQGQIFAAFPLRK
                     NLVQEVGVNMGIGRTFAEEDGYGRAWTPALELVGRQEMASGAKTDWDIVPQMQVSLST
                     LQHILVSAGYRIPVTNSSQRSGQFVFYFIWDWYDGGLLDHW"
     gene            13077..13616
                     /locus_tag="VGM72_03205"
     CDS             13077..13616
                     /locus_tag="VGM72_03205"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEY4264298.1"
                     /translation="MADVTTAPGYGFWHKSFHWIIFALLAAQYVVGSVMPHIGRNTQD
                     EGWVHWHFVIGAAILFFIVLRLIWRVLHPVPLMAMPGWQTWLASFTHTGLYVLILVMC
                     VLGWAANGDRGWTVYLFGIVPLPALAAKGTPWAHTAGDIHNVLVYVLLAFIILHIAGA
                     LYHTFVMRDRTLQRMLPGV"
     gene            complement(13663..15120)
                     /locus_tag="VGM72_03210"
     CDS             complement(13663..15120)
                     /locus_tag="VGM72_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HEY4264299.1"
                     /translation="MSRMDLDHDACYRAVCLRDSRFDGRFFTGVKTTGIYCRPICPAK
                     TPQSKNVTFYPTAAAAQEAGFRPCLRCRPETAPDLGAWRGTSNTVSRALALIELGALD
                     DARLDVLADRLGVGERQLRRLFRQHLGASPIAVAQTRRVLLAKQLIHETHLPMAEVAF
                     ASGFASIRRFNETFLSLFGRPPRDLRRAIGPDVPAGPLGEIDLLLRYHPPYDWPAMLE
                     FLSRRAIPGVESVTANRYARSVQLDGVQGTVVVQPTSQNALRATVRFPRLSALPAIIA
                     RLRRVFDLSADPVPIAAHLAKDPVLASLVKARPGLRVPGAWDGFELAIRAVLGQQITV
                     SAAARLGGRLAAMYGDRLTEPDGDLTHIFPRPEALAAADLTSLGMPRSRAATLCAVAA
                     ASMSDPHLFDATCGLDEAVRRLRSIRGIGEWTAQYIALRQLREPDAFPAADIGLMRAM
                     ANREGHGHSSHELLDRANVWRPWRAYAAQHLWASA"
     gene            15250..15819
                     /gene="ogt"
                     /locus_tag="VGM72_03215"
     CDS             15250..15819
                     /gene="ogt"
                     /locus_tag="VGM72_03215"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF007626.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY4264300.1"
                     /translation="MPVQIFSLERLNTPTGPILLATDDQHRLRALDWEDHEPRMQRLL
                     RRHYGADAIELRTALHPSAAGRSLEAYFEGDMDALTAQQTATNGTDFQRAVWDALRHI
                     PAGQTISYRVLAARIGRPAAIRAVGLANGANPVAIIVPCHRVIGANGSLTGYGGGLDR
                     KRWLLTHENASTPSGTSVRGDPAVASAAR"
     gene            complement(15876..17129)
                     /locus_tag="VGM72_03220"
     CDS             complement(15876..17129)
                     /locus_tag="VGM72_03220"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HEY4264301.1"
                     /translation="MESPDQTMPRAYVLDDEAEIRAFVCEVLRRNGFHAVQFSTMPSL
                     VEQLAVEPSEVLILDLALGQSDAVEVMQHLERLGYRGKILLISGRDGDILRDIQNIGR
                     SRGLSMLPPLGKPFRAGDLKQRLQANVEMREIAPGGPSKGSTVSIDLAEALHKDWLEL
                     WYQPKVDLKSLTICGAEALIRARHPEYGIVAPAGFLPPPNDPLYLPLSSFVIRRAMSD
                     WARFAAGGNPLKLAVNIPVSVISAPGFVNLVRASLPKDPSFRGLLVEVTENEVVQDTE
                     LFREIANQLKLYDVWLSIDDFGSAYSTLSRLLDLPFAEVKLDRAFVSNCGQDAMKAVV
                     CQTVIDLAHRFGADVCAEGIEDVEEARALRDRECDIAQGYFFAKPMPADGLLSTMLAP
                     NGPFDEQVHSIRNLDHCRSGTTIKS"
     gene            17663..>18276
                     /locus_tag="VGM72_03225"
     CDS             17663..>18276
                     /locus_tag="VGM72_03225"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY4264302.1"
                     /translation="MILATTILTAVSTFGNAARAVEDVDPFTLSPEELFDATVTSVSK
                     MPENLSDAPAAVFVITHDEILRSGAANLPDILRLAPNLQVAASNSDFYAVTARGFNGV
                     AANKLLVLIDGRTVYTPLYSGVFWDVQGVLPEDIDRIEVISGPGATLWGPNAVNGVIN
                     IITRSSRDTQGAVATVEGGSFENTARLRYGGKLGENATWRIYGMG"
BASE COUNT         3591 a         5340 c         5613 g         3732 t
ORIGIN      
        1 tgtcccgcga ggtcggcacg ttgccgacct ccatataatc ggggccatgg cagatgctgc
       61 actgaatcgt cttgccatta ccgcgcgcgg tcgtgagcac ctcgcccgcc ttgatgcttc
      121 tcatcggcac ataggcgacg aatggctaat gaggatctcg aacgttcgcc ctcttgcctc
      181 tcttgcggta cttcgatgat acgccgcccg atcggctcct tggcgctgcg tcggcgccca
      241 ttgttgcggc gagcaacagt aggaaaagaa gtgcaaagcc aatgttttat gagcatcctc
      301 cgaaaaacgc tgggatcctg ctgtaaagtc tattgcgggg caattacgag aacctcgtag
      361 ctggcttgca aggcttgtgg acgcccaccg gcaacggctg ggggatcgaa tttcgcggcc
      421 atggaataga tgcggccggt ggccgggtcg agcgttccag tgcgtgtccc gacctgagtg
      481 ggaacgtgct ggacgatgga gatatgtgcc gggtccgcga aggagagcac ttccaatgtc
      541 cccgcacggc agggaatgaa ggcgagcttg cgcgccgcat cgaagataac cgcatcggca
      601 cctttggcgg ttggaagcga tgcaacttcg gctccggtat cggcgcgtac gactttcgcg
      661 acaccgtcgc acgaggaaat caggagtttg tcggcggcgt cataggcaag acccgtgggg
      721 ccgtcgcaac cctttaacgc atagcgcgcg gtgaccttct tacccgccac gtcgatgacc
      781 gcgatctggt tcttgtcctc gacattgacg tagaccttgc cggcaccgtc cggtacggcg
      841 aattcgagtt caccgccgac agggattcgg gtcacgagtt ttttcgttgc cggatcgatg
      901 acgatggcat tgccggcctc gtgatccatc gcaaatatca tcttggagga aggatcatag
      961 gtcgcgccgt cgggattctt cccgcccttg aacgtcgcca caaccttgtc ggctgctcca
     1021 tcgacgagca gaacatcgcc gccgcgctgg gtcactgcca acagcgcact gcctggaatc
     1081 ggcagcgcga tatggccctt caccgcactc ttgagttcgc tcacctttcc agtcttgacg
     1141 tcgatcaccg tggtcatatc gggccgcgcc atgaggatgc gtccgttctt ggcatcgaac
     1201 gtggcataat cgaagccgcc gtcctgcacc ttgatgcggt cgacgatttt gtaattgggc
     1261 gctgccgctg cgatagccga cgtgctaaac gcgatcgaca gaatgagact gatgacggga
     1321 atctgtttca cggctgattc tccctttgac agttggccgg gtccatcatt ggcctggccg
     1381 cgttcttatc gaaacgtcgc gctcatcgaa catcgtaatc ggggccggga gcgccggtat
     1441 cggccgcggt cccaacggat agcttgcacg gaagccttgc gatgctttgg tctgcgcacc
     1501 gcggcgaggc caggaaggga tattccaggg gatggcccgg cgtctgagcc aaggtgtcga
     1561 gataattcaa tgggcccggc aacgggggtg gagctttggc cgtctcggta ttggcggagc
     1621 gctgcgtggg cgagggcccg tggaagccat ataaccgcgc gcccgctggg aagccgccgc
     1681 gcggtgccgc attcgcaata ttgggacaca gcgccaccgc aagtcccaac gccaacgtcc
     1741 tgaggatcgg agttcgcatc attcggccgg ttgtcgagcc gggccgtcga aggacgggct
     1801 gtcgctgccc gccattcgcg ttccggattt gcgagtcaaa acggaaaacg ccaggggcat
     1861 gatgagcaac accagcggca tttgaacgat aagtccggag atgatggcaa cggctagcgg
     1921 cttctgcatg tcggatccct gaccgagccc aatcgccagc ggcagaaggg ccagaatcgc
     1981 cgcgatcgtc gtcatcgcaa tcggccgaaa ccgattggcg ccggcctcga ttatcgcccg
     2041 ctcgtgggtc atgccttccc ggatgagcaa ttggtactcg gagaaataga agatcgcgat
     2101 ctcggtgacg attccgacca ccatggtcat gcccatcatg gccgaaatat tgagttcgat
     2161 acccgtgagc cagagcccca tgaacaccgc tcccatggcg ctcagcggca tcgcgatgat
     2221 gaggatcgcc aatgtcagtc gctcatagag atagatcaaa agcgcgaaga ccagggcgat
     2281 cgcagcaatc agaacgccga tgagaccgcg aaaagcgagc tgctgttggg cataaagacc
     2341 accaagctgg aaataggtgc cctgggtgaa cagaccggac ttgtcgagca atgctttgac
     2401 ctccgccgca gtgcttccca aatcgcggcc ttctatccgg gcggtgacgg caaccatcgg
     2461 cttgagattg tcgcgggtga tttccggttg accggtgagc aaactgacct gcgccacgcg
     2521 cctgagtggg actttgtggc cgtccggcgc ggtgatcagg aattcgcgaa tgtcgttggg
     2581 tgtcgcgcgc tccttgggcg gcacccagac gcgcacatcg atggttcggt cgacgtttcg
     2641 cacctgtgtc gcgaccgtgc ctgaaagata cgcattgatt tgttgggtca ccaaatcggg
     2701 gttgagacct tcaagcccgg ccttcagggg atccacgcgg atatcgaagg catcgcccgc
     2761 gatgacgacg ccattgcgga tctcagtgat gccgggaacg cccatgatcg catccgcgac
     2821 cttgggtgcg atctcgttca gcaccgccgg atcgtcgcca tagatctgga cttcaatggg
     2881 ctggggcacc gccgtgagat cgccgatcag gtcttccata agttgcgcgg tctcaacgtc
     2941 cagcgcgggg atcgtctttt gcgccttctg gcgtatttcc gtcatgacct cttcgatcgg
     3001 gcgccgggga gcaggcttca ggcgcacgaa aatatcgccg gtattggact ccgtgaggcc
     3061 cgcgccgagc tgcaaaccgg tacggcgcga ataggtcagc acttcgggcg tcgcgcgtag
     3121 aatcttctcg acctggttga gcatcgcatt ggtgtcttcg agcgaggttc ccggtggaga
     3181 gatgtagtcg aagacgaaac caccctcgtc catgatgggc atgaacccgg tgccgacctg
     3241 cgtgtaggcc aaatatccgg cggcaaggaa gatggcgaac accggcaatg cgagcaccgg
     3301 aaaggccaga agccctttga acgtcttgcg atagtagcgg agcacgcggc ggaaaaccgg
     3361 ccccgtgtgc tcttcggctt tggcgtcctt cgcggtcagg acgtaatcgg ccaagagagg
     3421 gatgacgaag aaagccgcca ggaacgacac gatcagcgcc gccgccaagg tcagcgacag
     3481 cgctttgaag aaggcacccg ttaccccggt cagaaacgcg agcggaacga agatgatgat
     3541 ggttgctgcc gaagagccgg cgagcggctg gaggaattcc caggtggccg cgcgtatttc
     3601 ggcatggccg cgttccccgg tgctgtgcag ccgccggtcg atctgctcga tgatgacgat
     3661 ggcatcgtcg atgatcaggc cgatggctgc cgccatccca cccagtgtca tgatgttgaa
     3721 actcatgccg agcacggaaa gcagcaatac cgtcgaggca agaaccgcgg gcaccacgat
     3781 cagcgtaatc aatgtgatct tgagattgcg caagaagaga agaagaacga gccccgcaag
     3841 cgcaacgccg atcagcacgg cctcgaacac actgcgcgcc gatcccaaaa tcagctcgct
     3901 ctgatcgtac caattgctga tgtgaaaatc gggcggcagc ttgggtgcgt aggcggtaag
     3961 cgcggctttc acgtcattga cgatctggac cgtattgcca tcctgctgct gtttgatctg
     4021 cagaataatg gcgggatgtc cgtctcccgt cacgatggtc cagcttggcg ctgcggtttc
     4081 gtacactctg cccacattgg acagatagac ggcaccggcg ggccctccgc gcacgatcgt
     4141 ctgacgaatt tggtcgggtg tcgtgagttg attgtcggtg agcgtaagaa gcagcttatg
     4201 tcggtcctgg acgcgcccta cggcctgcag cacattcgcc gccgtcaaag ccttggacac
     4261 gtcgtccatg gtaaggccgt aactcgccag ccgcgccgga tcgatatcca cccggatttc
     4321 tcgtgcggct ccgccaagaa tctctacgcg ctggacaccc ttgatcgagg aaaccagcgg
     4381 cagaagttcg tattgcgcga tctcccggat cttcacgagg tcaagtttgt cgctagtaag
     4441 gctgtaggcg gcgaccggaa agacatttgt attcatgcgc cggatatcga acgtcgcccc
     4501 ggccggaagc gacggcaaca cgcgcgcaat ctcggcctgg acctgaaggg tggcgacagc
     4561 catgtcgatg ccccaatcga agttgagcga cagttcggcg gtgccccggc ttgtggtcga
     4621 gcgcacttcc agaaggccgg gcacggcctt gaccgcctgc tcaaccgggc gtgtcacctg
     4681 ggtgaccatc tgcgccgccg gctgatcgcc agcatcgatc gtgatcgcta tgcggggaaa
     4741 attcacttcc ggaaagagcg caaccggcaa accaaacgca ctgaacgcgc cgccaatcgc
     4801 gacgaccgcc agaagaaaca ggatcgatcg gcggtgctgc gcagcccatt gcgctaaatt
     4861 catggctttg cctccttgac gggagctgct gcctgggccg cgggcgcggc ctccgccgat
     4921 ggctggcctt ccacctgcac cggcgtgtca tcgtccaatt cggaattgcc cgagatgacg
     4981 acttgcgtgc cgagggcggg gtcgagccca tcgataatcg ccatgtcgtc ggtttcgacc
     5041 aggaccttga tgtcctgtct cttggcttcg ccttccgaaa ccgtgaagac ataggagccc
     5101 ttggcgtctt cgagcagtgc catacgcgga atcacgatgc cctgtaccga aggaaggtcg
     5161 atgtgcgcga gcaatgtcgt ccccaacgcc agatgggcgc tgttggccgc cggaacgttg
     5221 gcgatggcct tcaccagatg ggtcatcgga tcgatgcttg cgccgatcga cgtgagtttg
     5281 ccggcgatct cgctcccatc gccaaatgag acggtgagcc gcaccgctgc tcccggcgcc
     5341 agtttcgatg cgtcttgggg atcgagattc agctcaatca ccacgtcgga gcgtttcgcc
     5401 acggtggcga tcgtggtgtt cgcttgcacc ttgtcgcctg gcgtcgccat gagccccgtg
     5461 atcacaccgt cgaagggagc gcgcagcgtt acctccgttt cacccgcgcc gatcatgcgt
     5521 tgctggtcca attggacctg tgcgtcggcc aagattttat gcgccgtggc aagttgatcg
     5581 ttggttgcaa gcttgttggc aaacaagcgc tcgacccgcg tgagatcctg catcgcaaaa
     5641 tcgaccgcct gttttgcctg caagaacgca gcgacgttcg cgggcgcggt cgtgacggtg
     5701 acgataggtt ggcccgcccg caccgcatcg ccgtcgcgca cattaacgct cttgaagacg
     5761 ccgtcccgca tcagggcgat gacgaatgcg ttctgtggcg atgacgccac cgtgccatag
     5821 gcgagagcca ctggcgcaag cgatccggag gttggggtcg ccaacgttac tgtcgccgta
     5881 ccttgtattt cttcctcttc ggcttcaccg gccggtcttt cgttgatccc gaacaatccc
     5941 gggaacacca cgatggtgat cacggcggcg gtcaccagca gcgcgcccac cagcaaaatc
     6001 cacctgagaa gtggatgctt catggcatca gttccttcgt ttcaataacg ggctcgattt
     6061 gcgtgccaag tacgctgctg agcgcaattg catcgttcca gagggactgt ctgagatcgg
     6121 aaagttcgga ctgacgatta agaagcgaac tctgcaacgt aacgtaggtc aaggccggca
     6181 aatcgccggc aagataggcg cgttcgccat tttccgatgc catctgaagt tccggcagtc
     6241 gggcatctat gtcccgtatc gcctcgacca attgttgcat ctcggtccat aagcgccacg
     6301 catcgcctgt cgcctggttc agccgcgcct gatactccgc gaggagctgc gcgcgtgtag
     6361 cactctggac cgcgatgtcg ccttgattgc gatcgaacaa cggcagattg agcgtcgcac
     6421 ccaggcttag cgtatgaaca ttcgtcgtat cgttcgcagt cccaagaccg aacgagagac
     6481 tgggaaactg cgccaacacc gatcggcgaa aggtctcttc ctggctctgg tagccgaatt
     6541 gaagggcacg cagatcgggc cgcgtttgcg cgaccttggc gagcgcggca tcgatctcgg
     6601 cgctggttgg aatcgggggc ggtgtcaggg gttggagcgg cacatcaacc ttgggggcca
     6661 ctccgagcag cgcccgtatg ttataatctg tttgcgattg gatacgctct gcggtgcgca
     6721 gctgactgcg ggcgtccaga agtgcggcaa gatcgatgcc cgcctggtca aaggtgacat
     6781 ctccggcttg cagagcgcgt cccgaccgct ccgcctgcgt cgcgtatcgt ccttcgagtg
     6841 cggcaaagag ttcgcgcttc tcagccgcgt tgatacgctg gacataaagc gtgcgcgctt
     6901 gcgccaccgt ctgccattcc tgccagagca gactaaggcg cgcttcgtcg cgcgccgcat
     6961 ttgctgccgc aagcctagcg ggttggacga caagcgcctg gatatcgtat tcgataccga
     7021 ggccgtatcc cgtgacgacg ctccctccgc caatcggtgg atcgagattg gcggtgagca
     7081 cgggattggg caagatgccg gcggcgaagg cttgtgcttc agcgaccccg gccttgagac
     7141 gcgcggcctt caaatccgga ttgttgagaa ccgcgacggt cgccaccgcg ttgatgtcca
     7201 gcggttgcgc cgcctgcgcg ggaatccgct ccgacaaatt gaccgtttcc ggcagcggga
     7261 cgggggcata ggtcgaatag gtggcgcagc cggtaagcgc aaagaccgcc ccaaggacgg
     7321 cagcgagatt tccggcggca cgactaagat gacgccgaag catcgcaggc aaattcccat
     7381 atttccgcat gaagacaact gtttctaacg gtatcagaac ggatgttatg gcgacgttgg
     7441 gagaaggttg ggaatttgta acctagctcg cgagacattg tgactcgcat tcggaatagc
     7501 attacgattg cgggagaccg actggaggac gcggtgagaa tcctggtggt ggaagacgat
     7561 gcggagaccg caagttacct ggtcgccgga cttgaggagg aggcgtttac ggtaaggcgg
     7621 gcggcaaatg gccgtgacgg cttgcttatc gcgatgaccg aagcgttcga tcttctgatc
     7681 gtcgaccgaa tgcttccgca actcgatggt ttgaccctcg tcaagacgct gcgtactgcc
     7741 agtattcaga cggcggtgat ttttctgacg actatgaccg gcatcgacga tcgcgtaacg
     7801 gggctgaatg ctggcggcga cgattatctg gtgaagccat ttgccttctc cgaactggtc
     7861 gcgcgcgtgc gcgcgcttag ccggcgtcct cgcggcacgg cagtggaaac gcgtttggcg
     7921 gtggccgatc tcgagctcga ccttttgaag cgcgtggtca aacgcaatgg caacgaaatt
     7981 cagctccagc cacgtgaatt ccgtctgctg gaatatctca tgcgtcacgc cgggcaggtg
     8041 attacgcgaa ccatgcttct ggacaatgtt tggaactatc atttcgatcc gcgcacaaac
     8101 gtggtggaat cgcatttgag ccgcttgcgg accaagatcg acaagggatt ttcgccggaa
     8161 ctgatccaca cggttcgcgg cgccggatac gttcttcgtg cgccggaata gacttcttca
     8221 cgcatccagt ttccggctgg gactgcttta tgccggcctt cttacagcct ccgtacttgt
     8281 ccttttcggc gtcacctatt ggttcgcgac cgactacgct gcacgcgatg agaccgatga
     8341 gatcggcgtg gagttcgcat caattcaaga cgaggtacga tcctcaggcg aagcccgtct
     8401 acccacgatc atcgacaatc atgtgcgttt gcgcaaagac gttcgcgccg tctatctgct
     8461 ccaagacccc tcggggcata agatcgccgg caacatcgag gccatgacac cgtttcttgg
     8521 ccccaagatc ctggatcgta tttttgatgg agagaaacgc caggtacgag cctatggcta
     8581 caacctcagc aatggcgact atctgctcgt aggccaggat acggccacgc ttcgacagat
     8641 gaaggcgctg atttttcgct cattcggtat tggtttcatc gtcaccgttc tgttggcgat
     8701 tctcggcagc gtgatcgtca gcagcacagt gctcaagcgc gtcgagacgg tcggccgcac
     8761 agcgcgcgcg attatcggcg gtgatttttc gcagcgtgtg cctgttcgcg gaacggacga
     8821 cgaattcgac gaactggccg cgagcatgaa tgcgatgctc gatcgcatcg aggacctgat
     8881 gcgcagcatg cgtcaggtat ccaacgatat cgcccacgat ctgcggacgc cgttgacccg
     8941 gttgcgccaa aggctcgagc acgcaagacg gcgcacgcat acagtggagg aattgcagga
     9001 agccttggga cattcaatcg cccaggtgga ttcgatcctg gagacttttg gcgctctgtt
     9061 gcgcattgcg caaatagagg ccgacacccg gtcagcaagg gaagtttcgg tcgatgtctc
     9121 gaagctgctc tcgggaatcg tcgacgattt cgcaccggcg gccgaggatc acggtcaaca
     9181 gctggtcgcg gaaatcgagt cgcggttgag cgcgaaagcc gatccggaat tgcttacgca
     9241 gatggtggtc aacctagtcg agaacgccat tcggtattcg cccgtaggtg cccggattgc
     9301 ggtacaggcg cgcgcttcgg caggatattt ggaactggcc gtcagcgata cgggcccggg
     9361 cattccgatg caggagcgcg agaatgtgct gcggccattt taccgcctgg aggcgagccg
     9421 gaccaccgaa ggcagcggcc ttggattgag tctcgttgcc gcgatcgcca aacgtcatca
     9481 ggcaaaactg agcctgaacg acaacgatcc gggccttcgg gtcgtggtcc tgttcccggt
     9541 tgcggcctga cgccggcgaa tccttcagcc gcccgggctc gtcttgcgtc gacgatttgg
     9601 ccgatagcga ggccttcgcg tgagcaagac tgcgtgacgt caggaaataa tattgatcgg
     9661 tgcggagaag tttgttggcg gtgagcggcg catcgttttg ggggatggca agtatgctac
     9721 ggctgttgaa gattatcccg gcgtctgact caaacgcgcg tgaagttgaa gaccggtgaa
     9781 tgccaaatgt tgcgtcctta acgccgaccg gttgattggc tctaacacat gttacgacaa
     9841 tcgcattgca agtgatattc agtgtgtcag ccattccgtc ccgcgctcga tagtctatcg
     9901 tccaggcttg tcatcggtgc gcccgcgtca tgcacaatca aaagatggcg cttcaatgtc
     9961 gtccggaaca ccgaggctcg ttaaggggaa gaggaggtcg tgagagcgct gcgagcaacc
    10021 agtatggcgg atgcacccgc tgcctgcgtg tagtagctca caaagcgctt gggctccggc
    10081 gcatcgtaat tgcgcagatt ctgcgcccag cgatagccga tcgactggta caggagttcc
    10141 gccgtcacat tatagggcgc gcccgcattc ccattgggaa gtgctgcgct gtaggtcagc
    10201 gaatccgaac cgccggtgaa atcgctgtcc tcgcgcgcgg ggcccacgac catcacgcgt
    10261 ggctcggcgt tcgtcttgtc gaagccgcgc ggcagaaggc gattgtcctt cagataatgc
    10321 gtcccataga gcaggcccgt cgtcacccgg tttgcaaaat cgcccatgat cgattcatag
    10381 atctgcacct gatccgtact cgtgatgcgc gtgtaatgcg gttcgaattt cgttgcgtcc
    10441 tcgtcattgt cgttgcccgc gatcgagcca tcgggacggg aagcgcctga ttcgaacacc
    10501 agcgcacccc ggctatcgcg caccacgaca tgcagccaag cgcgccgtgc cggataggcg
    10561 gtcgggaatt tgtggccggc cttgtttgtg accatgacac cgaattccaa ccgccccgcg
    10621 gtgactgtgg cattcttgat cgtgagggaa gccgtctcgg tccccagttg attctcggtg
    10681 cgcttggccg atgcttccag ctcgtcggcc gtcgcgatga cgccgagttc cttgcgatga
    10741 tctttcagta tccgcagcag gaacgcattg ccgccgacga agacatgccg cgacacttcc
    10801 tgatgcgtct ggctgtgcac cgaggtgatc ggcgcatcgc ccgcgacgcg tggcatgtgg
    10861 caggattggc aggaattggt ctgcgcgtaa tcgctgtgct gccattcctg atagggcacc
    10921 tgttcgggca aggtgccggc gggattgcct ttgtcgtcca gcgcacgcgt caacagggtg
    10981 tgacaggtcg cacataattc ggattgcttg atgtgctcgg tcttggtggg cgtgaagccg
    11041 gtcaccgaat gcatgattcg cgtgcggcct ttgtcgacat cgaagggacc gaaaatctca
    11101 cgctcttcgg gcttttttgt gagatcgatc acgaagccac cgtcgaggct cgattcctgc
    11161 ccgaaattgt cggggcgtac ctgatgacac aatgtgcagg agacgccgtc cgccgcgagc
    11221 gtcgctttcg gctccgccgc gtcttcgagc ttcgcttcgg gttcgacatt tgccatccct
    11281 gaccggatgg cttcgagata gcggaagact tcgccgcttt ggttctcggc acgcgcctga
    11341 aagcgctgca tcggcatgtg gcaggtggag caggtgtctt cgatgatcgc cttggcctgc
    11401 ggatgatcca tggtctcgcg gcggatgccg gcctgccaat acgggtcgcg cgcggaattc
    11461 gccatgatgg acgcgcgcca ctgatagccg atcgagatgt cctcgccatt cggcgcgaag
    11521 actttgctgt ggcaggcgat gcagtgaagc gaagtcgtga acagcatgtg atccaccggc
    11581 ggatgcgccg atgcgccgcg ctccgcggcc cggccatagc caatcgccaa acctgtcagc
    11641 gttgtaacaa ttcccaaagt accggacagg acgacaaggc tccttctctt gcgtatcagt
    11701 cgcaacttta agctcaacgc cttgttcctg cggatcattc gcatgtaatc ctgtctatct
    11761 gttcttgcgg atcacttgca ctatgcttgc aagtctagag aaaatcataa tagcaacgtc
    11821 tgtattgctt caagtcaaaa gcatttcgta ctcctgttac cttgggagcc gcacgagcgt
    11881 ttcgctgccg cgcggcatgc acatcaccga ggggagatga cggcaatttt cattcttcga
    11941 cctgcggccg ggttcatcgc cgccagctcc gcgtttttat tgctcgcaat cgtagcggcc
    12001 gcatcgcagc cggcgccgcc ggatgggcat gcgatcttcc tggcgaattg cgcgacctgc
    12061 cacggcgtgg atggccgcgg catgcgcacg ccggccgaag tgggcttcga ccttcccatg
    12121 cccaatttca gcgattgcag tttcgctacg cgcgaggccg acgccgactg gtcctccacc
    12181 attcacagag gcggccggca acgcgcgctc gcccgcatca tgccggcgtt tgaagatgcg
    12241 ctctccgacg acgagatcga tgctgtgatc ggttatctca gaactttctg caccgatccg
    12301 cgttgggtgc gcggcgaatt caatttcccg ctcggcctct tcaccgaaaa ggcgtttctg
    12361 gaagacgagc tggtctggac gaccgccatc gatgcgaaat cgcccaacaa catccaatcg
    12421 caattcattc tggagaagcg catcggcgct accggccaac tcgaattcac gctgcccttc
    12481 gcccgcatgg acggcggccc cggtgtcgga cgaaatttcg gcgtgggcga tttcggcgtg
    12541 gcgtggaagc agaacgtagt cgcggatgtc gattccggta cgatcttcag cgtgctgggc
    12601 gagatggtgc tgccgacggg aagcgaaaaa aagcagctag gcaccggttc gttgtcattc
    12661 gaaacccatg ccttgttcgc gcaaatgctg cccgacgatt ttgtctatca aggccagatc
    12721 ttcgccgcct tccccctgcg caaaaatctt gtccaggaag tgggcgtgaa tatggggatc
    12781 ggcaggactt tcgctgaaga agatggctat ggccgcgcct ggacaccggc tctcgaactc
    12841 gtgggccgcc aggaaatggc gagcggtgcc aagaccgact gggatatcgt cccacaaatg
    12901 caggtgagcc tttcgacgct tcagcatatt ctggtcagcg cgggttatcg cattcccgtg
    12961 accaatagct cgcagcgcag cggccaattt gtgttctatt tcatctggga ttggtatgac
    13021 ggtggcctgc tcgaccattg gtagtacgcc ttgcctgtct tgaatgggag atttccgtgg
    13081 cagatgtgac gaccgcgccg ggctatggct tctggcacaa aagtttccat tggatcattt
    13141 tcgcactcct cgcagcacaa tatgtcgtcg gctcggtgat gcctcatatc gggcgcaata
    13201 cccaggacga gggatgggtg cattggcact ttgtcatcgg cgcggcgatt ctgtttttca
    13261 ttgtgttgcg gctgatctgg cgcgtcctcc acccagtccc gctcatggcc atgccgggct
    13321 ggcagacatg gctcgcgagc ttcacccaca ccggcctcta cgtgctgatc ttggtgatgt
    13381 gtgtgctggg ctgggccgcg aacggcgacc ggggatggac ggtctatctt ttcgggattg
    13441 ttccgctccc cgcactggcc gccaagggaa cgccgtgggc gcatacggcc ggcgacatcc
    13501 acaatgttct cgtttatgtg ctgctcgcct tcatcattct gcacatcgcc ggggcgctct
    13561 atcacacatt cgtcatgcgc gaccgcactc tgcagcggat gctgcccggc gtatgatcgt
    13621 ctattttcct cgagaattca gaaaccgcaa atacgtcctc attcaagccg atgcccaaag
    13681 atgttgggca gcataggcgc gccaaggtct ccagacattg gcgcgatcga gaagttcgtg
    13741 tgaggaatgg ccgtgtcctt cccgattggc cattgcccgc atgagcccga tgtcagcggc
    13801 gggaaaggcg tccggctcac gcaattgacg caaagcgatg tactgtgcgg tccattcccc
    13861 tattccacga atcgaccgca gccgccgcac cgcctcgtcg aggccacaag tggcgtcgaa
    13921 cagatgcggg tcggacatag aggccgctgc aacggcgcag agcgtggccg cccggctgcg
    13981 tggcatgccg agggatgtga ggtctgctgc cgctaaagcc tcgggccgcg gaaatatgtg
    14041 agtcagatcg ccatccggct ccgtcaagcg atccccatac atggcggcca aacgcccgcc
    14101 taggcgcgca gccgcggaaa ctgtgatctg ttgtccgagc accgcgcgga ttgccagctc
    14161 gaagccatcc caagcgcccg gcacgcgcaa tccaggccgt gccttcacca gggaggcgag
    14221 tacgggatct ttcgcaaggt gggcggcgat cgggaccgga tcggccgata agtcgaacac
    14281 ccgccgcaag cgcgcgataa tggccggcaa tgctgacagt ctgggaaatc tcaccgtcgc
    14341 gcgaagcgcg ttctggctgg tgggctggac cacgaccgtt ccttgcacgc catccaattg
    14401 taccgatcgt gcgtaacggt tggcagtgac ggactcgact ccggggatgg ctcgccggct
    14461 gaggaattcc agcatggcgg gccagtcgta aggcgggtga tagcgcaaca gaaggtcgat
    14521 ctcgccaagg gggccggcgg gaacgtccgg gccgattgcg cggcgcaagt cgcggggcgg
    14581 gcggccgaaa agggacagga atgtttcatt gaaccggcgg atactggcga atcccgaggc
    14641 gaacgcgacc tcggccattg gcaggtgcgt ctcatggatg agttgcttgg caagcagcac
    14701 gcggcgggtc tgggccacgg cgattggcga tgcgccaaga tgctggcgga agagacgccg
    14761 gagctgccgc tcgccgacgc caaggcggtc ggctaaaacg tcgaggcgag catcgtccaa
    14821 ggcacccaac tcgatcaacg ccagcgcgcg cgacacggta ttggatgtcc cgcgccaggc
    14881 gcccagatcg ggcgccgttt ccgggcggca gcgcaaacac ggacggaagc cagcctcctg
    14941 tgccgccgct gccgttggat aaaacgttac gttcttggac tgcggtgtct ttgccggaca
    15001 gatcggccgg cagtagattc cggttgtttt gacgccggtg aagaatcggc cgtcgaaacg
    15061 ggagtcgcgt aggcaaaccg cgcggtagca ggcatcatga tcgaggtcca tacgggacat
    15121 ataggcggtg tcgcggcaat ttctagcggt tttcggacat cgtcatggaa atgatgacca
    15181 tgtccgaaaa ccgccagcgg atggcttcgc gctgtccata tctgtcatga ccaacgcgga
    15241 gtgatcgcca tgcctgttca gattttttct cttgaacgtc tcaacacacc taccggcccg
    15301 attctgctcg ccaccgatga ccaacatcgc ctgcgggcgc tggactggga agaccatgag
    15361 ccgcgcatgc agaggctgtt gcggcggcac tatggtgcgg acgcgatcga gctgcgcacc
    15421 gcgttgcatc cttcggcggc tgggcgatcc cttgaagctt atttcgaggg cgatatggat
    15481 gctctgaccg cccaacaaac cgcgaccaat ggcacggact tccagcgcgc ggtttgggat
    15541 gcattgcgcc acattcctgc cggacaaacc atcagctatc gcgtcttggc ggcccggata
    15601 ggccggccgg cagcgattcg cgccgtagga ttggcgaatg gcgccaatcc cgtagccatc
    15661 atagtcccct gtcatcgggt tatcggcgca aacggctctt tgaccggtta tggcggcggc
    15721 ttggatcgca agcgatggct gttgacgcat gagaatgcaa gcacgcccag tggcaccagc
    15781 gtacgaggcg atccggcggt ggccagtgcg gctcgatgaa tggcgggagc ggaagacgaa
    15841 atccgtttaa tgcttgcgtt cgttcgcaca gggactcacg atttgatcgt ggttccagac
    15901 ctgcaatggt ccaggttccg gatgctgtga acctgttcgt cgaacggacc gtttggcgcg
    15961 agcatcgtgc tcaagagccc atcggcgggc atgggtttgg cgaaaaaata cccctgtgcg
    16021 atatcgcatt ccctgtcacg cagggctcgc gcctcttcga catcttctat gccttcagca
    16081 cagacatcgg cgccgaaacg atgcgcaagg tcaatgaccg tctgacagac cacagctttc
    16141 attgcgtcct ggccgcaatt tgagacgaac gcccgatcca gtttgacctc ggcgaaaggc
    16201 aggtcgagca gccgggacag ggtagaatag gccgatccga aatcgtcgat ggaaagccag
    16261 acgtcataga gcttcaattg attcgcgatt tctctgaaca attccgtatc ctgaacgacc
    16321 tcattctcgg tgacctcgac aagaaggccg cggaatgacg gatccttcgg caaggaggcg
    16381 cggacgagat tgacgaagcc cggcgcgctg atgacggaga ccgggatatt caccgccaat
    16441 ttgagcggat tgccgcccgc agcgaaacgc gcccaatccg acatcgcgcg ccggatcaca
    16501 aacgaagaca gcggcagata aagcggatcg ttcggcggcg gcaggaagcc cgccggtgcg
    16561 acgatgccat attccgggtg ccgcgcgcgt atcaatgctt ccgcgccgca tatggtcagc
    16621 gacttcagat cgactttcgg ctgataccac agctccaacc agtctttgtg gagcgcctcc
    16681 gcgagatcga tggaaacggt ggaacccttg gatggcccgc caggcgcgat ctcccgcatt
    16741 tcgacgttcg cctgaagtct ttgtttcagg tcgccggcgc gaaatggctt gccaagcggt
    16801 ggcagcatcg acagcccacg gcttctgccg atgttctgga tgtcacgcag gatatctcca
    16861 tcccgtccgc tgatgagaag aattttgccc cgatacccga gacgctccag atgctgcatc
    16921 acttcgacag catcggactg accgagggcg aggtcgagaa tcagaacttc ggacggttcg
    16981 accgccagtt gctccaccag cgaaggcatg gtggagaatt gtaccgcatg gaagccgttg
    17041 cgcctgagca cttcgcacac gaacgcacgg atttccgcct cgtcgtcgag aacgtaagcg
    17101 cgcggcatgg tctggtccgg cgactccact atgactccta ggtgttcgac cctcatggag
    17161 aaattaggtt caatgtttgg atttttgatt aaggcaagtt ctcgccacgg gcggtcgaaa
    17221 aaaggcatga tcaacgccat gccccccgtt attccgttcc gcaagatgcg tgtgctgagt
    17281 ccgcggtgca cttcctgcgg atagcgttgg cccatcattt tcgcgaatgt ttggcagtca
    17341 cgatcgattt ggccgcacgg ccgggatgca agccgttcac ggcgacgccg gcgggccttc
    17401 caggattctg acacccattc gggaaagacg cggtcaccgc tttaatgaat cgttaactac
    17461 gcaccaagga gaatccgaat cgtaacctga agtgttacgc cgcagccaag ggggactcgg
    17521 ccgccggcgt cttggggtga gcaatctcgc tcagcgaagg acgcagcata tgatcccggc
    17581 aagttccggc aaagattcga cgttgcgccc cccgttgagc tgcctggcgg cgaccagagg
    17641 caaacgcttc gagggacgtg ggatgatcct cgctaccacg atattgaccg ccgtatcgac
    17701 cttcggcaat gcggcgcgcg cggtggaaga cgttgatccc tttacgcttt ctccggaaga
    17761 gcttttcgat gccactgtca cttcggtgtc caagatgccg gagaatctga gtgacgcgcc
    17821 tgctgcggtc tttgtcatca cacatgacga aatcctgcgt tcgggtgccg caaatcttcc
    17881 ggacatcttg cggctggcgc cgaatcttca ggtggcggcg tccaattccg atttttatgc
    17941 agtgacagcg cggggtttca acggggtcgc cgccaacaag ctgctggtgc tcatcgatgg
    18001 gcgcaccgtc tacacaccgc tttattccgg agtgttctgg gatgtgcagg gggtgctgcc
    18061 tgaggacatt gaccggatcg aagtgatcag cggtcccggc gccacgctct ggggtcccaa
    18121 cgcggtcaat ggcgtgatca acatcatcac acgcagttcc cgtgatactc agggcgcggt
    18181 ggcgacggtc gagggcggta gttttgagaa taccgcgcga ctgcgctatg gcggcaagct
    18241 cggcgaaaac gccacgtggc gcatctacgg catggg
//
LOCUS       DASYEG010000087        11625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2344595, whole genome shotgun sequence.
ACCESSION   DASYEG010000087 DASYEG010000000
VERSION     DASYEG010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 11625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11625
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2344595"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="VGM72_03230"
     CDS             complement(<1..1452)
                     /locus_tag="VGM72_03230"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY4264303.1"
                     /translation="MAKARSIFMPSVSRTLSRFVNAAHGLPSAVHRSLSPVTAAEAGI
                     IESQLDFVQASLRHLDWALPLAGLAVMVTVRAYGIAVAPIAACFAALIVNCVLNEFLL
                     TRRIASCGDIVADTKRRAYRIAAMALILSIVWCAMVFSMYHSEITANRMFVVLILGCT
                     MGSLSTMFAMHTAAAAGSMLVVAASLVTILSWNMLSGRLTLLPIGAIYIVLVVNQVAG
                     MNLRFRQTKRLEQEREDLIGRLQDANIQSMTAQERATKANKAKSEFLANMSHELRTPL
                     NAIIGFSEVMREQMFGALGHSRYHEYSRLIHGAGMHLLGLINDVLDMSKIEAGKLDLH
                     LEKFDLRDVIGDCLDLMGERAGAARIELVSELSGTPLPLLADRRAVSQMVLNLLSNAV
                     KFTLPGGRICVRAANIDSRAIISVEDNGVGIAADDLHRLGKPFVQVRNQAGILHAGTG
                     LGLALVRALAEKHAGAMRIESQETVGTTVTIELP"
     gene            complement(1580..2356)
                     /locus_tag="VGM72_03235"
     CDS             complement(1580..2356)
                     /locus_tag="VGM72_03235"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY4264304.1"
                     /translation="MSSSAARALRILDWIARAGRPLGVSEIAREASLAPGTVFRSLDA
                     LVRADFIARYQASARYVPANAVERLRRSVIAGFRMREPVLPYLRQLASASGETVSLHV
                     RLGWYGVRIASALGTAEVTSAANLGETQPLGDYYAGKAMLAFLSEAHIARYGDWLAGH
                     GVPFREQARELHAVKKRGFASGDADFTGARAVAFPIRSTQEPIAAIAIEGPVLPRTGE
                     HHGGFSDWRETVAHVEALARAQAGLFENPFAHLDPNSLIL"
     gene            complement(2353..3183)
                     /locus_tag="VGM72_03240"
     CDS             complement(2353..3183)
                     /locus_tag="VGM72_03240"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY4264305.1"
                     /translation="MSLAEKSVPGPVRYRNQATGRVLSVLLAFTNSSQPRGVAELSRA
                     LGMNKNMVHRALTTLTAEGYLVRDVSGARYQLGPRLLTLPAGALHESDIVALARPALE
                     QLHALTGESVYLSIIVGRNRVTVDDIQAQGARVLRSARGHPVPLHCTKMSRVLLAHLS
                     DADIAAYLAAAAPLARASRFPDPPSESERGIWQDIAAIRAVPFLLWRNPHASSAAYAI
                     FPVCDASGHPHAILTVGGPRERFDLAEIEARLPRMRAILEPLEREARLFPAPIFLAEI
                     "
     gene            3271..4077
                     /locus_tag="VGM72_03245"
     CDS             3271..4077
                     /locus_tag="VGM72_03245"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4264306.1"
                     /translation="MDGQAPETDRIDCAGVSVELLQRGEGQPILFLHPGHPAGRVDPV
                     ARMVELLAQRARVIVPTHPGFGMEAAPPQLTTVDDLAYLYLDLMDAMDLRDTVVVGAS
                     IGGWIAAEMAVKNCERISRLVLADTVGIKTGGRESRDIADIYAITDRELAERVYADPA
                     RMARDPKSLAPAALKLMARSRESTGRYGWTPYMHDPKLKGRLHRIRVPALVLWGDADR
                     VVTPDYGRAFAAAIAGARFDSIAGAGHFPHLEQPDAFARAILDFIEEIRR"
     gene            4074..5267
                     /locus_tag="VGM72_03250"
     CDS             4074..5267
                     /locus_tag="VGM72_03250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY4264307.1"
                     /translation="MRVFQFTEQPYPDVWNDHNGSLRVNLPNRKLDPKRAADLFHRYY
                     DEWQLADELGLDIMLNEHHSTATCMSAACVVGLSVLARITKRARLLVLGYPIANRPDP
                     LRAAEELAMIDVISRGRLEMGFVRGVPQEVAIANLNPVFQSDRFWEAHDFIMKALTSH
                     DEPFNWESENFNYRHVNIWPRPYQQPHPPVWSTTGSTYTAKKLGGYGYVMATLGTGYD
                     TRRLYDAYRQGYAEKREGAPAPADRFAYLGLVAVGRTEQDARRRGELIASYPRSSAIV
                     FAPFRNPPGYLSIEDNVRILKGEGKPRSFTKDGRVVEMSKGSVQDLIDAGVMFCGTPD
                     QVHDQIVDFAEHCGGMGNLLMMGHAGPMTHEDTVENLTLFAQEVYPRLKTLHQPETAA
                     ADAAQ"
     gene            5273..6088
                     /locus_tag="VGM72_03255"
     CDS             5273..6088
                     /locus_tag="VGM72_03255"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4264308.1"
                     /translation="MRNIESRFADIAGMRTHYLEGGRGPHVVLLHSGEFGGCAELSWE
                     FTLSALAEHFHVIAPDWLGFGESAKIFSFDGMRALRVRHIAQFLQTLNIEKAHFIGNS
                     MGGGMLAGVAAQDAPLWPIDRMILASAGGFAPVNEARRILNSYDGTREHMKRILETTL
                     AHSPLRSDEAYLEKRHRLSLVPGSWECTAAARFRRPGVTSRDKEEIDYRNIKRPTLIV
                     AGGKDPLREPGYGQELQTDIAGSELLVFPEAGHFPHIDLPERFNRAATAFLSR"
     gene            complement(6080..7603)
                     /locus_tag="VGM72_03260"
     CDS             complement(6080..7603)
                     /locus_tag="VGM72_03260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY4264309.1"
                     /translation="MVENQNEQDDVHAVAAKSASGAFTAFQSVEQELIQTKEALERKT
                     NELALAKERYALAEDATDEGLWDWNPLTRENYLSARWKAMLGFAEHELSDIDDTFFSR
                     IHPDDETRVREALHLHFEKREPYNTEVRLRCKDTSYRWFRTRGKSIRDDSGRVIRMVG
                     SIFDATEQKHADQKLRDSEARFRGTFDNVAVGMVHLSLESVYLMVNQCFCDIVGYSKE
                     ELIGKTFRDITHPEDFRNDVAEANEILAGTIDFFTREKRYLRKNAEPVWVSLTVSLQR
                     DQAGRPQYFIGVVRDITGRKRTAQHLEFLIQELSHRSKNLLAVVQAMANHSARTSASM
                     TDFRRRFSQRLQGLAASHDLLVQQNWQRGDLAELVARQLAPFDEIADGRLDAGGPGVS
                     LDAGAVQNIGLLFHELATNASKYGALSVPEGKVIIRWTLDPAEAKSRCLRLSWTERGG
                     PPVETPAHKGFGLIVIERTVAAALNGSVSVEFAPEGLACTLAIPTSHVICRRDARSA"
     gene            complement(7871..9724)
                     /locus_tag="VGM72_03265"
     CDS             complement(7871..9724)
                     /locus_tag="VGM72_03265"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY4264310.1"
                     /translation="MTKLHSMLLASAAAVVTGLGMTASAQAADQLLTGAIASAAGQKL
                     DGVVVSAKRDGSTITTSVYTDATGNYYFPPMAAGKYQVWAQTLGFEQTKVAVDLEGTK
                     HQDLMLKTIADPETKWKQLPGELVWDALPEGNAEDVHMKQIIHNNCNGCHTPSYPLQF
                     KFDEQGWSRVIDLMKVIGGGLPQDRPANQIIQMNQKRLAAYLARVRGPNSPTPNIPTR
                     ARPSGEAARVVWTLYDVPRVQGAGASALPPSTADNDGSNWALGTPSKAALIIHDDQMD
                     FDGNIWFTSNSMNSLVTVGKVNAKTGEISYYKWPRASNPALASPTHGITRDADGNLWF
                     DVNPGRRSLGKMEPKTGKITVYPTPETMMPLGGAVTMDVDGKGMVWASAPAGVLKFNP
                     KTLEFTEFKTTDVKRSYGTGGTYGAAGDRSGNGYWAEMGFDTIGKADFATGKVVEIKL
                     PENKKIRDFLTPQEVAAYGKVTDISTGNPYPWAQGPRRMGTDKAADVLWVGNSWGSSL
                     ARIDTKTNGVEMIPFPDAAMQPYHISVDSKHNVWGNLWTNDEIFKYEPTTKKWTMFEL
                     PVRGTEMRHLTVDERNGLKISMPVYRTNQMGVMIIRSDEDIAKLKQQVASR"
     gene            complement(9746..10102)
                     /locus_tag="VGM72_03270"
     CDS             complement(9746..10102)
                     /locus_tag="VGM72_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY4264311.1"
                     /translation="MKRHAALFVFLLASGAQAASAQSLTPQQAHGRQILTQNCNICHL
                     PQNPGSATYGPRLSKSSANGDDKLMKQVIQTGLVKMPGWRYALKDSDIDDVIAYVRTL
                     PEPPPPTTRGGGLGAD"
     gene            10313..>11625
                     /locus_tag="VGM72_03275"
     CDS             10313..>11625
                     /locus_tag="VGM72_03275"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4264312.1"
                     /translation="MAVILDTDTVINKSRIGRLQIMVGVLGAAILFVDGFNTQVIGYI
                     APQIARNWNIPRDVLGWILAADKVGLLIGYLFVAPFSAYFGHKRVAIGCIVTFGLLAL
                     ATTVADNAAVLFVLRLLTGIGLGGALPSGVVLTGEYFPLARRSSSITFIYCGFSFGQG
                     SAGEVANAVLQPYGWQAAILIGGGLALALAATLAVLLPESLEYLVNRGGNPQTAAKIL
                     RRIAPDIAIGPETRLVAGARNGATLTFWQLFPQLFQMRRGFGTLVIWLALGMNLIVNT
                     SLQVWLTKILQDSGFAQHTAIIATEASFAAGIVGAFIIGPLIDRFGPYRVMGALFVVG
                     ALFSLFIGLSLSWGVAATIIAASFGSGFCTSGVQKGGNALSVYFYPVALRSTGLGWGL
                     GIGRIGAILGPLAVGYLLTSGWPAPTVFAMMAVPMLIGASAIFAMG"
BASE COUNT         2153 a         3629 c         3552 g         2291 t
ORIGIN      
        1 gggcagctcg atcgtgacgg tggtgcccac cgtttcctgg ctctcgatgc gcatcgcgcc
       61 cgcgtgcttt tctgcaagcg cgcgcaccag agcaaggccg agcccggtgc ccgcgtgcag
      121 gatgccggcc tggttgcgca cctgcacaaa gggttttccc aggcgatgca aatcgtccgc
      181 ggctatgccc acgccattgt cttcgaccga aataatcgcg cgcgaatcga tattggcggc
      241 gcgcacacaa atgcgtccgc ccggcagcgt gaacttcacc gcattggaaa gcagattcag
      301 caccatctgg ctgaccgccc gccggtccgc caacagagga agcggggtgc cggaaagctc
      361 actcaccaat tcgatccttg cagcgccggc gcgctcaccc atcagatcga ggcaatcgcc
      421 gatcacgtcc cgcagatcga atttctcgag atggaggtcc agttttccgg cttcgatctt
      481 ggacatgtcc agcacgtcat tgatcagtcc gagcagatgc atgccggcgc cgtggatcaa
      541 tcgggaatat tcatgatagc ggctatgtcc cagcgccccg aacatctgtt cgcgcatcac
      601 ctcggaaaac ccgatgatgg cattgagcgg cgttctgagt tcgtggctca tattggccag
      661 gaattcggat ttggccttgt tcgccttcgt cgcccgctcc tgggccgtca tcgattggat
      721 attggcgtcc tgcagcctcc cgatgagatc ttcgcgttcc tgctccagac gcttggtctg
      781 acggaatctg agattcatgc cggcgacctg gttcaccacc aggacgatgt agatcgctcc
      841 tatcggaagc agagtaagcc ggcccgacaa catgttccag gacaggatgg tgacgaggct
      901 ggccgcgacg acgagcatgg acccggccgc cgccgctgtg tgcatcgcga acatcgtgct
      961 caacgatccc atcgtgcagc ccaggatgag aacgacgaac atgcggttgg cggtgatctc
     1021 ggaatggtac atcgagaaca ccatggcgca ccacacgatc gaaaggatga gcgccatggc
     1081 ggcgattctg tacgcgcgcc gtttcgtgtc cgcgacgata tccccgcaag acgcaatcct
     1141 gcgagtgagc aggaattcgt tcagcacgca gttcacgatg agcgcggcaa agcaggcggc
     1201 gatcggcgcg acggcaatgc catacgcgcg gaccgtcacc atgaccgcca gaccggccag
     1261 cggaagcgcc caatccaaat gtctgaggct cgcctgcacg aaatcgagtt ggctctcgat
     1321 gatgccggct tcggccgccg tcacgggact gagcgagcga tgaaccgccg aggggagtcc
     1381 gtgtgccgca ttcacaaagc gcgatagcgt tcgcgaaacg ctcggcatga atatgctgcg
     1441 tgccttcgcc atgacggttc tattacgttc gcagcatgtc gagaactttc attgcgtcat
     1501 gatcgcgcgc cagcagttaa gcttcgctta agctacacgc gcggctgcga gaattggcca
     1561 aggccgcatg ggcctcagct cacaggatca gggaattggg gtccagatgc gcaaacggat
     1621 tttcgaacag accggcttgg gcccgggcaa gcgcctccac atgagcgacg gtttcacgcc
     1681 aatcggaaaa tccgccgtgg tgctcgccgg tgcgcggcaa aacgggacct tcgatggcga
     1741 ttgccgcgat cggctcttgc gtggagcgga tgggaaacgc gaccgcgcgg gcacccgtga
     1801 aatccgcatc gcctgacgcg aaaccacgct tcttgacggc atgaagctcc cgcgcctgct
     1861 ctcgaaacgg aacgccgtgt ccggcaagcc agtccccata gcgcgcgatg tgcgcctcgg
     1921 aaagaaacgc cagcatcgct ttgccggcat aataatcccc cagaggctgc gtttccccga
     1981 gattcgccgc gctcgtcact tcggcggtgc cgagggccga cgcgatgcgg acgccgtacc
     2041 agccaagccg gacatggagc gacacggttt cgccgctcgc cgaagccaac tggcgcagat
     2101 agggcaacac cggctcgcgc atgcgaaaac cggcgatgac gcttcggcgc aagcgctcca
     2161 cggcgtttgc tggcacgtag cgcgccgatg cctggtaccg cgcgatgaaa tcggcgcgca
     2221 cgagggcgtc caggctgcga aacacggtcc caggcgcgag cgatgcctcg cgcgcgatct
     2281 cgctgacgcc gagcggccgg ccggcgcgag caatccaatc caatatacga agcgcgcggg
     2341 ccgccgagct gctcatatct cggccaaaaa aattggtgcc gggaaaagac gggcttcgcg
     2401 ttccagcggc tccagaatcg cgcgcatccg tggcagccgg gcttcgatct ctgcgagatc
     2461 gaaacgctcg cgcggtcccc cgacggtgag gatcgcgtgc ggatgtcccg aagcgtcgca
     2521 caccggaaaa atcgcatagg ccgcgctgga ggcatgagga ttgcgccata gcaggaaagg
     2581 cacggcacgg atcgcggcga tgtcctgcca gataccgcgt tcgctttcgc tcggcggatc
     2641 gggaaaacgg ctggcgcgcg ccagcggcgc cgcggcggcg agataggccg cgatgtccgc
     2701 atcgctcaaa tgcgcgagca gcacgcgcga catcttcgtg caatgcaggg gaacgggatg
     2761 accgcgcgcg ctgcgcagaa cgcgcgctcc ttgcgcctgg atatcgtcga ccgtcacacg
     2821 attgcgtccg acgatgatgg agagatagac gctttctccg gtcagggcgt gcaattgctc
     2881 aagcgcgggc cgcgcgaggg caacgatgtc cgattcatgc aaagccccgg ccggcaacgt
     2941 cagcagtcgc ggcccaagct gatagcgcgc gcccgagaca tcccggacca gatacccctc
     3001 ggccgtcagc gtggtgagcg cgcgatggac catgttcttg ttcatgccga gcgcgcgcga
     3061 aagctcggcg acgccgcggg gctgcgaaga attggtgaag gccagaagca cgctgagaac
     3121 ccggcctgtc gcctggttgc gatagcgaac cggcccgggg acggatttct cagcgaggct
     3181 catggatgtt tgacaggcaa agcgctgtct cggtaggatg aaaagtaaaa ttcttttcac
     3241 atactataac gaatggggag gtggggcaac atggatgggc aggccccgga aaccgatcgc
     3301 atcgactgcg ccggcgtaag cgtcgaactg cttcagcggg gcgaaggcca gcccattctg
     3361 ttcctgcacc ccggccatcc cgccggacgc gtggacccgg tcgcgcgcat ggtggaactg
     3421 ctggcgcaaa gggcgcgcgt gatcgtcccg acccatcccg ggttcgggat ggaggcagcg
     3481 cccccgcagc tcacaacggt cgatgacctc gcctatctct atctcgatct gatggacgca
     3541 atggatttgc gcgacaccgt cgtggtgggc gcctccatcg gcggctggat cgcggccgag
     3601 atggcggtca agaattgcga acgcatatcc cgtctggtgc tggccgatac ggttggaatc
     3661 aaaaccggag gccgggaaag ccgcgacatc gccgacatct atgccatcac cgacagagag
     3721 cttgccgaac gggtctacgc cgatccggca cggatggcgc gcgatcccaa atcgcttgcg
     3781 ccagccgcgc tgaaattgat ggcgcgctcg cgcgaatcga cgggccgcta cggctggacg
     3841 ccttatatgc acgatcccaa gctgaaaggc cgtctgcatc gcattcgcgt tccggcactg
     3901 gtgctgtggg gtgacgcgga tcgcgtggtc acacccgatt atgggcgtgc cttcgccgcg
     3961 gcgattgcag gggcgcgctt cgactccatc gcgggcgcgg gccatttccc gcatcttgaa
     4021 cagcccgacg cgttcgcgcg agccatactg gatttcatcg aggagatccg ccgatgagag
     4081 tcttccaatt caccgagcag ccctatcccg atgtgtggaa cgaccacaat ggctcgctgc
     4141 gcgtcaatct tcccaatcgc aaactggatc ccaaacgcgc ggccgatttg ttccaccgct
     4201 attacgacga gtggcagctc gccgacgaac tcgggctcga catcatgctg aacgagcatc
     4261 attcgaccgc cacctgcatg agtgcggcct gtgtcgtggg tctgtcggtg ctcgcgcgca
     4321 tcaccaaacg ggcgcggctc ctggtgctgg gctatcccat cgccaaccgg cccgatccgc
     4381 tgcgcgccgc cgaagaactc gccatgatcg acgtcatctc gcgcggacgg ctggaaatgg
     4441 gtttcgtacg cggcgtgccg caggaagtcg cgatcgccaa tctcaatccc gtgttccagt
     4501 cggaccgctt ctgggaagcg catgatttca tcatgaaggc gctcaccagc cacgacgagc
     4561 cgttcaactg ggagagcgag aatttcaact accgtcacgt gaacatctgg ccgcgtccct
     4621 atcagcagcc tcatcctccc gtgtggagca cgacgggaag cacgtatacg gccaagaagc
     4681 tcggcggtta cggctatgtc atggcgacgc tcggcaccgg ctacgatacg cgcaggctct
     4741 atgacgccta tcggcaaggc tacgcggaaa agcgcgaagg cgcgcccgcg cccgccgatc
     4801 gcttcgccta tctgggcttg gtcgcggtcg gtcgcaccga acaagacgcg cgccgccgcg
     4861 gcgagctgat cgcttcctat ccgcgttcgt cggccatcgt gttcgcgccg ttccgcaatc
     4921 cgccgggtta cctctccatc gaggacaatg tccgtatcct gaaaggcgag gggaaaccgc
     4981 gaagcttcac caaggacggc cgcgtcgtgg aaatgtcaaa gggcagcgta caggacctga
     5041 tcgatgccgg cgtgatgttc tgcggcaccc ccgatcaggt gcatgaccag atcgtggatt
     5101 tcgccgaaca ttgcggcggc atgggaaatc ttttgatgat gggccatgcg ggaccgatga
     5161 cccatgagga cacggtcgag aatctcacgt tgttcgccca ggaggtctat ccgcgtctga
     5221 agacgctgca ccaacccgaa accgccgctg ccgacgcggc tcaatagacg cgatgcgcaa
     5281 catcgaaagc cgattcgccg acatcgcggg catgcgcacg cattatctcg aaggcggacg
     5341 cggccctcat gtggtgctgc tgcattcggg cgagttcggg ggttgcgccg aattatcctg
     5401 ggaattcacc ctgtccgctc tggccgaaca ttttcatgtc atcgcacccg attggctggg
     5461 tttcggcgag agcgccaaga tcttcagctt cgacggcatg cgggccctgc gcgtccggca
     5521 catcgcgcaa tttttgcaaa cacttaatat cgaaaaggcc catttcatcg gcaattccat
     5581 ggggggcggc atgctcgcgg gcgtcgcggc ccaggatgcg cccctctggc cgatcgacag
     5641 gatgatcctt gccagcgcgg gcggcttcgc cccggtgaac gaagcccgcc ggatcctcaa
     5701 ctcctatgac ggcacgcgcg agcacatgaa acggattctg gaaacgacgc tggcgcattc
     5761 gccgcttcgc agcgatgaag cctatcttga gaagcggcac cgtctttcgc tcgtgcccgg
     5821 ctcatgggaa tgtacggcgg cggcgcgctt ccggcggccc ggcgtgacaa gccgcgacaa
     5881 ggaagagatc gattaccgca acatcaaacg gcccacattg atcgtcgccg gcggcaaaga
     5941 tccgttgcgc gaacccggct atgggcaaga attgcagacc gacatcgccg gctccgaact
     6001 gctggtgttt ccagaggcgg ggcattttcc acatatcgac ctgcccgagc ggttcaatcg
     6061 ggcggccacc gcgtttctgt cacgctgacc tcgcgtccct ccggcaaatg acgtgagacg
     6121 tcggtatggc cagcgtacac gcaaggcctt ccggggcgaa ttcgaccgat acggagccat
     6181 tgagtgccgc cgcaacggtg cgttcgatca caatcaggcc gaagcccttg tgcgccggcg
     6241 tctcgacggg cggaccaccg cgctcggtcc agctaaggcg caagcaacgc gatttggctt
     6301 cggccgggtc gagtgtccat cgaatgatga ccttgccctc cggcaccgac agcgcgccat
     6361 atttggaggc gttggtcgcc aattcgtgaa acaagagacc gatgttctgg acggcgcctg
     6421 cgtccaggct cacgcccgga ccgccggcat caagccggcc gtcggcgatt tcatcgaacg
     6481 gcgcaagctg gcgggccacg agttcggcga ggtcgcctct ttgccagttc tgctggacga
     6541 gaagatcgtg cgatgcggcc aaaccttgca ggcgctgcga gaagcgccgc cggaaatccg
     6601 tcatggaggc gcttgtcctc gcggaatggt tggccatggc ctgaaccacc gcgagcagat
     6661 tttttgaacg atgcgacaat tcctggatca aaaactccag atgctgggcc gtgcgcttgc
     6721 gtccggtgat gtcgcgcacg actccgatga aatattgagg ccgccccgcc tggtcacgct
     6781 gcaacgagac ggtcaggctg acccatacgg gttcggcgtt cttgcggaga taccgcttct
     6841 cgcgagtaaa aaaatcgatc gtgccggcaa ggatttcatt cgcctccgcc acatcgttgc
     6901 gaaaatcctc ggggtgggtg atgtcccgga acgtcttgcc gatcaattct tccttgctgt
     6961 agccgacaat gtcgcaaaaa cactgattga ccatcagata gacgctttcg agcgacaaat
     7021 gcaccatgcc gaccgcgaca ttgtcgaagg tgccccggaa acgcgcttcg ctgtcccgca
     7081 atttctgatc ggcatgtttc tgttcggtgg catcgaagat cgaaccgacc atgcgaatga
     7141 cgcggcccga atcgtcgcga atgctcttgc cgcgtgtcct gaaccagcga tagctggtgt
     7201 ccttgcagcg gagcctcacc tccgtgttgt agggctcgcg cttttcgaaa tgcaggtgaa
     7261 gcgcttcgcg cacgcgcgtc tcatcgtcgg gatggatgcg ggagaaaaac gtatcgtcga
     7321 tatcggacag ctcgtgctcg gcgaaaccca gcatcgcttt ccagcgcgcc gataggtaat
     7381 tctcccgtgt cagcggattc cagtcccaca ggccctcgtc ggtggcatct tccgcgagag
     7441 cataacgctc cttggccaga gcgagttcgt tggtcttgcg ctccagcgct tcctttgtct
     7501 ggatgagttc ctgttccacg ctctggaacg cggtaaaggc gccggacgcg ctttttgccg
     7561 cgacggcatg aacgtcatcc tgctcgtttt ggttttctac catcacggcc ttcgatcttt
     7621 cccccggccg ccgcggtgtt ccgcaaacag ggccgtgcga gacacaaccg gaaaaatcca
     7681 ttcgacgatg gagcaagtcc gagtctcgtt tacgatgcgg acttagggtt tataaaaggt
     7741 taccgaacaa acctccaagc ctgtcaagcc cagtcttctc cgcccaccat gacgttcccg
     7801 aaacagaacg ggcgggccac ctggcccgcc cgttgtaacg cactgcgctg ttaagacagc
     7861 gcacccccca ttagcgggaa gcgacctgct gtttcagctt cgcgatgtcc tcgtcgctgc
     7921 gaatgatcat cacgcccatc tgattggtgc gatagaccgg catgctgatc ttgagaccgt
     7981 tacgttcatc gaccgtgagg tggcgcatct cggtgccacg aaccggcagc tcgaacatcg
     8041 tccacttctt ggttgtgggt tcgtatttga agatctcgtc gttggtccag agattgcccc
     8101 acacattgtg cttggaatcc acgctgatgt gataaggctg catcgccgca tccgggaagg
     8161 ggatcatctc gacgccgttg gtcttggtgt cgatgcgcgc gaggctcgag ccccaggaat
     8221 ttccgaccca aagcacgtcg gcggccttgt ccgtgcccat gcggcgcggg ccttgcgccc
     8281 acgggtatgg gttgccggtg gagatgtccg tcaccttgcc gtaagccgcg acttcttgcg
     8341 gcgtgaggaa gtcacggatc ttcttgtttt ccggcagttt gatttcgacg accttgccgg
     8401 tcgcgaagtc ggccttgccg atcgtgtcga agcccatttc cgcccaataa ccattgcccg
     8461 aacggtcgcc cgcagcgcca taggtgccgc ccgtgccata ggagcgcttg acgtccgtgg
     8521 tcttgaactc cgtgaactcc aaggttttgg gattaaattt gagcacgcct gccggcgcgg
     8581 acgcccagac catgcccttg ccatccacat ccatcgtcac ggcgcccccg agcggcatca
     8641 tggtctccgg cgtcgggtag acggtgatct tgcccgtctt cggctccatc ttgcccaggc
     8701 tgcggcggcc agggttcacg tcgaaccaga gattgccgtc cgcatcgcgc gtgatcccgt
     8761 gcgtcggaga cgcgagcgcc ggattgctgg cgcgcggcca tttataatac gagatctcgc
     8821 ccgttttggc gttgaccttg ccgaccgtga ccaggctgtt catgctgttc gaggtgaacc
     8881 agatgttgcc gtcgaaatcc atctggtcgt catggatgat gagagcggcc ttcgaaggcg
     8941 tccccagcgc ccagtttgag ccgtcattgt ccgcggtgct cggcggcagc gccgaggcgc
     9001 cggcgccctg aacgcgcgga acatcataca atgtccagac cacgcgcgcc gcctcgcccg
     9061 atggacgtgc gcgtgtcggg atattgggcg tcgggctgtt cggtccgcgc actcgggcga
     9121 gataggccgc gagccgcttc tggttcatct ggatgatctg gttcgctgga cggtcctgcg
     9181 gcagtccgcc gccgatgacc ttcatcaggt cgatgacccg gctccatccc tgctcgtcga
     9241 atttgaactg caacggatag ctcggcgtgt ggcagccgtt gcagttgttg tggatgatct
     9301 gcttcatgtg gacgtcttcg gcgttgccct cgggcaacgc gtcccagacc agttcgcccg
     9361 gaagctgctt ccacttggtt tcgggatcgg cgatcgtctt cagcatcaga tcctgatgtt
     9421 tcgtcccctc gagatcgact gcgaccttgg tttgttcgaa gccaagggtc tgagcccaga
     9481 cctgatattt gcccgctgcc atcggcggga aataataatt tcccgtcgcg tccgtataga
     9541 cgctggtggt gatggtcgat ccgtcacgct tggccgaaac caccacgcca tcgagcttct
     9601 ggcccgccgc cgaggcgatc gcgccggtca aaagctgatc cgcagcctga gccgatgccg
     9661 tcattcccag cccggtcacc accgcggccg cacttgccaa tagcatgcta tgcaatttgg
     9721 tcatggtgtt tctcctgtga ggatttcaat ccgctccgag gccgccgcca cgggtcgtgg
     9781 gaggcggtgg ttccggcaat gtccgtacat acgcaatcac atcgtcgata tcggaatctt
     9841 tgagggcgta tcgccagccc ggcatcttca cgaggccggt ctggatgacc tgcttcatca
     9901 gcttgtcatc gccattggcc gaggacttgc tgagccgcgg accgtatgtc gccgagcccg
     9961 ggttctgcgg caaatggcag atgttgcaat tctgggtgag aatctgccgg ccatgggctt
    10021 gctgcggagt caaactctgg gccgaagcgg cctgtgcgcc cgaagccaat aggaacacga
    10081 acaatgcagc gtgacgcttc atcaggcgtt ctcccattac ttatttttta caagtctaat
    10141 ccccggtgag gccttgggga aggcctccat gcaagaattc actccaattt atgccacaaa
    10201 agcaagattc atgcgggcgc agggctctat ccgatcattt ggcgcttccc gggtttacgg
    10261 tctaggcttc ggaaaaatca cggagaaatc cgggcgttcg ggagcagtta cgttggccgt
    10321 tattctcgac accgatacgg tcatcaataa aagccggatc ggccgcttgc agatcatggt
    10381 cggcgtgctg ggcgcggcca tcctgttcgt cgacgggttc aacacccagg tcatcggcta
    10441 tatcgcgccg cagatcgcca ggaactggaa catcccgcgc gacgtgctgg gctggattct
    10501 ggccgccgac aaggtcggcc tcctgatcgg ttatcttttc gtggcgccct tttccgccta
    10561 tttcggccac aagcgcgtcg ccatcggctg catcgtcacg ttcgggcttc tcgccctggc
    10621 gacaacggtc gcggacaatg cggccgtctt gttcgtcctg cgtctgctca ccggcatcgg
    10681 acttggcggc gcgcttcctt cgggcgtggt gctgaccggc gaatattttc cgctggcccg
    10741 ccgctccagt tccatcacct tcatctattg cgggttttcg tttggccaag gctccgctgg
    10801 cgaagtcgcg aatgcggtgc tgcaacccta tggctggcag gcggcgatcc tgatcggcgg
    10861 cggtctcgcc cttgcgcttg cggcgacgct ggcggtgctt ctgcccgaat cgctcgaata
    10921 tctcgtcaat cgcggcggga atccgcaaac cgcggccaaa attctccgcc gcatcgcgcc
    10981 cgatatcgcc atcggtccgg aaacccggct cgtcgcggga gcgcgcaatg gcgccacgct
    11041 gaccttctgg cagttgtttc cacagctttt ccaaatgcgg cgcggcttcg gcacgctggt
    11101 gatctggctg gcgctcggca tgaacctgat cgtcaacacc tcactgcagg tgtggttgac
    11161 caagatcctg caggattccg gatttgccca gcacaccgca attatcgcga cggaggcctc
    11221 cttcgcggcg ggtattgtcg gggcctttat catcggcccc ctgatcgacc gcttcggacc
    11281 ctatcgcgtg atgggagcct tgttcgtggt cggcgctttg ttcagcctct tcattggtct
    11341 gtcgctgtcc tggggggtgg cggccacgat catcgcggct tcgttcggct cgggcttctg
    11401 caccagcggg gtgcaaaaag gcggcaatgc gctgtcggtc tatttctatc cggtggcgtt
    11461 gcgctccaca ggactcggct ggggactcgg catcggacgc attggcgcaa ttctggggcc
    11521 gctggcggtg ggatatctgc tgacgtccgg ttggccagcg cccactgtgt tcgccatgat
    11581 ggcggtgccg atgctgatcg gcgcaagcgc catcttcgcc atggg
//
LOCUS       DASYEG010000088        14252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2200927, whole genome shotgun sequence.
ACCESSION   DASYEG010000088 DASYEG010000000
VERSION     DASYEG010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 14252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14252
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2200927"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="VGM72_03280"
     CDS             <1..757
                     /locus_tag="VGM72_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4264313.1"
                     /translation="KTVQLDFGQSFRYNEPVLRVIAERMPVSIYYGIVTAIFTFAICI
                     PLGILKAIRHRSVVDNLSSALIFIGYAIPGFALGAVLVNLFAVQFQVFPLGGFQSQGG
                     EALPPVAKFVDIVWHSVLPLVAYLAGAFAIETMLMKNSLMENMSADYVRTALAKGLTW
                     RRAVFVHALRNSLIPMATSIGSLLGIFLTGSVLIERVFNIQGVGLLFFEAIQARDFPV
                     VMGFLVISSVILMLGNLVSDLAVAFVDPRVRFE"
     gene            762..2126
                     /locus_tag="VGM72_03285"
     CDS             762..2126
                     /locus_tag="VGM72_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY4264314.1"
                     /translation="MTWIKLDPITRRRFARFRQIRRGYYSFLILTAAILLSVFAPFLA
                     ESRALLVVYQGRVFLPTFQYLTMAIFHQTPPPGWSSADLDTDYNRLQHEWRVERFFYE
                     RERAEAGSDPAKLSALETKYPDRRSYVIMPLIPWDPYQSDFWYNEILNDIQGLLTAGN
                     REGAERLARQDDLEDLANAIQTGTIDTILKDPKRSPSGDLAGLARSGTMSSLKGLAQV
                     PPTGPDLRRGHYFGTDSQGRDVASRLLYGFRISIFFALFLTISGQVIGTIIGSLQGYL
                     GGGFDLASQRVIEVLISIPFLYVVIILAALIVPNFWTLLLIMAVFQWIAITFYMRTEM
                     YREKTKEYCLAAKSYGASHMRIIFRHLLPNCLTPLVTFTPFAIVASIFALTGLDYLGY
                     GLPAPTPSWGEMIDQALRPENRDKLWLTLAPFGAITITLVLVVFIGESVREAFDPKRY
                     AKYE"
     gene            2140..4056
                     /locus_tag="VGM72_03290"
     CDS             2140..4056
                     /locus_tag="VGM72_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4264315.1"
                     /translation="MNLTRRSVALMVSAAALFRGQLSWPAESGKISAPAPAGPALLPK
                     DIVWETANDEPPIGSDKAMRGGTLNLSISSYPLTFRLYGPNNNDSFATWNRAFTIGFS
                     LVQMHPVNDKFLPMLATQWAVEKDQKTLYFKLDPDARWSDGKPITAGDYVFAWKMLQS
                     KFIVDPFANTYMKEYFASVDKIDDYTLRIVGTRPSWRPLYDYNFSPMPAHAIVLDENW
                     VTRTNNQFPVAVGPYVISGVVRGESVTFKRLPNWWGDKKRYFIGLYNFDEIHLRVIPT
                     DRELDYLRQGELDMMAEPSVKNWHEVYTFPAVTNGWIRRARVFVDTPTGVNGFQMNLE
                     APVFQNKDFRTAMQYLVNFDRLNKNLWYGEYFRVNSFFEGTIFANPDVKARPFDPAKA
                     REYLERAGYRRPDSVRNQGVWAQLRNVAYGLLFTRSDTDDILVNDKGEKASFTLMYLY
                     KALENEMTVIQQDFRRAGVDMRLQLLEPGAAFQRMLERKFEMGFVNMTSGYYPDPRQY
                     LHTDFKNSKNNNDFWGFGTKEVDDLIGVYEKDLDPKARMDAMHRIDQIIHDEAFTIPF
                     RTAPFIRLVYWDYVQFPDFYLPLRTQQYMDYMVYWIDPAKKAALEEAMKTGKTYPVDT
                     NMDQDYYGVRKKFQ"
     gene            4096..5094
                     /locus_tag="VGM72_03295"
     CDS             4096..5094
                     /locus_tag="VGM72_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4264316.1"
                     /translation="MALLEIENLQVGFDTEAGFVHAVDGVSCRIDSGRTLGLVGESGC
                     GKSVTATSILRLVPNPPGKIYGGEIRFNGTDLLKLKSEELPKIRGKEIAMIFQDPMTS
                     LNPVFTIEKQMGEVLKLRFGMDRHAARQRSVEMLGTVGIADPASRLTNYPHELSGGMK
                     QRVMIAMALLCEPKLLIADEPTTALDVTIQAQILHLIRELQRRTGAAVLFITHDMGVV
                     AEMCDEVAVMYAGRVVEQGDVVDIFKRSRHPYTKGLLRSLPQKGQQKKAELPTIEGVV
                     PSLLDPPQFCRFADRCWKRKRLSEDLQKKCWSEDPQLRSVESGLVACHYPLAGEEP"
     gene            5091..6098
                     /locus_tag="VGM72_03300"
     CDS             5091..6098
                     /locus_tag="VGM72_03300"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEY4264317.1"
                     /translation="MSAPVDTPLLTVEGLKKYYPVRGGVFSAKIGDVRAVDGVDLTVR
                     RGETLGLVGESGCGKSTLGRTILRLEEPTAGRVLFEGLDIARASKSELFNLRRNMQVI
                     FQDPYSSLNPRMTVGEIVREPLLVHRIGTKAEQIEKVRVLLETVGLTGEMLNRYPHEF
                     SGGQRQRIGIARALALDPQLVIADEPVSALDVSVRSQVLNLMVRLQRERNLTYIFISH
                     DLSVVEHLSDSIAIMYLGRIVEKGPAERVFAKPAHPYTRALLEAIPVADPTRRRDSIP
                     VRGEAPSPVSPPPGCPFHPRCAYAIDACHTIVPPLEPVGIPNLVPPLGHVAACIRKNE
                     I"
     gene            complement(6107..6487)
                     /locus_tag="VGM72_03305"
     CDS             complement(6107..6487)
                     /locus_tag="VGM72_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264318.1"
                     /translation="MRTKIIRHTRHGSCIASIAAAILAMSCMSASAAAQPSAVNAIPL
                     EDDVVPAPNGYDATSAKYTVARPTDIYISPFIWGGKVKGVHFDAGQPVDILARPKGYD
                     WFLVGKNGTGIGYVPMANVAPAKP"
     gene            6669..7010
                     /locus_tag="VGM72_03310"
     CDS             6669..7010
                     /locus_tag="VGM72_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264319.1"
                     /translation="MKRIALFAATALVFAPSAAIQVRAADTSPGKVCLNASQIQRTEV
                     PDDRTIIFHMRDGKIWRNTLKTACPMLKISPYTQKLNGDLVCSNQQFIHVVMTGNDCV
                     LGDFTPLPDER"
     gene            7097..7972
                     /locus_tag="VGM72_03315"
     CDS             7097..7972
                     /locus_tag="VGM72_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264320.1"
                     /translation="MTAHVHSRRLLRASLAALVLCAGAVMPTLRAQPVAGVSGAVPDF
                     SSNRMTWVLMNGTAFFRVPGDTGPGPIMQRGKEYKHDDVPRIAETDNPILKPWAKKLM
                     DINNARVLAGDIPFYTTSRCWPGGVPGLLLYPGEPVVFAQTPKEVWILYQRDAQVRRI
                     YLNVPHSKNPAYSWYGESVGHYENGDTLVVDTIGLDDKGPIDRFRTPHTKQLHVVERY
                     RLNNGGKNIEVTFSVEDPGAFTMPWRAKVDFARGRGARSGAWEESICADGPVDYFADS
                     IPSAVPIPRADKPDF"
     gene            8144..11380
                     /locus_tag="VGM72_03320"
     CDS             8144..11380
                     /locus_tag="VGM72_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4264321.1"
                     /translation="MKSTSLRSHTCNAVRLAALLGSASFMAMAATEAAWAQGQATPPQ
                     NQTAQALAAMPPEQVLVTGSLIQGAAAVGVPVSDLRPTDFVETGKLSITEVLKSVPSL
                     RIDAEASPTYGGGTLSFLQNVQIHGLGTGSGVETLLLVNGLRWPPQNYSNDTVNPSII
                     PQIALERIDVLSAGASAVYGSDATAGVINLILKRGYDGAMTQASVTSSPSTGYLQEQV
                     SQLYGKSWDSGNITVSYAFTDSRAVSAGRRSYYTQDFTPWGLMDLTPLGSSIPGIAHS
                     GNAATPAGTPALETAASGTMFCSNCFSIPKGQDGTGLTWAAIMANPGVKNERNPWIDS
                     DARPRLQTNAVTGTLDQQLTNDFFGLGPVALFADGFYSNQRGKQIYTPGNGQARNVLE
                     TNLPIPASNPFRPTGAPANLRVDYSFALEAPLYVTGGEVATHWDAGLNLDKLPFDWIG
                     KLTYSMSDDRNYGDATNSINKNNLLAALGNTVTIPATSTATSYSFVKPSAVPYLNVFC
                     DPSAFTCNDPATLAYIAGYRLQHETFKIQETGFNFNGPIFDLPGGPVKLAFAGQTLSE
                     HWTYLNLENDNTDSTAVVQNAPDAAYQSSYAFFTQADIPIFGQANAIPFFQSLVVELG
                     YRYDKYNNLNDPVWTPKAAVNWDVGAGLTLRAAWGKSFRVPSFAENSVGGSRVAGYNP
                     LGGFSNATNVTVLDCKSVNGSPAGVALPGSLTAVLNPTCSTDPSLVQPGGVSVELSGN
                     GAAAVLRGHGLSPQTLNQWSTGFNFSPNDSILGGALNGLNFDISWFRLEFKGLISSNG
                     LGQGGNDPVSFARYTVIPRPDLPITASENAAFLNLVTALAAVPQRSGFTFDPAFIPNI
                     KFIQDIALTNIGSRVFSGIDFDVRYDFDLGRVGLTDMGALNIGAAGFYETIDKSRADA
                     GQPLVSVFEGKDSGNRLQRVRYRLGWSDDTWSVTAFANYFGHGANTATLGVNFAGNNL
                     IPPCYYSAGFGPGSCYPGSPFYGPYSTYPNMAPAMVYFDLTIGYQTGEKPANEYLRNI
                     GVQFTVNDIFDRPPPFEVGARGNGSIRAFENSFSDLQRTFTLTLTKSW"
     gene            complement(11454..12596)
                     /locus_tag="VGM72_03325"
     CDS             complement(11454..12596)
                     /locus_tag="VGM72_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264322.1"
                     /translation="MNTKIMATASIAALLVGLSLPVPAAEQTTIFNTKDFHQDRTLWS
                     DPAYYKNNTAGQLRGMAIGIVPYENTGQVGSSRVYNTEGTGKAGAANLGSPYPFKTAK
                     EHYEAWLKDAHGGIKHTKATIPDWSGHWNGGGGGFGGGGSPASDVVKLLTPKYQEYLV
                     QELKAATEGRIWSANAFCLPNGFFPTLAASEFIATPDRVWTLTEGNGWNGIRWIYTDG
                     SGHTPEDLQFPKWAGESIGFWNGDTLIVYTNQMKAWKGGLEEYTDNLETVEKYRRVGD
                     QIQGEITLYDPEVLVKPVTSRLIYRLSKDNRPEKRPLYNTCSDSNGPSPKVFMDANGL
                     LNEHVPGDPGFNWSGADSRPWGTWLTESDRRYKAYIAAGGKPPGQK"
     gene            complement(12656..13024)
                     /locus_tag="VGM72_03330"
     CDS             complement(12656..13024)
                     /locus_tag="VGM72_03330"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HEY4264323.1"
                     /translation="MKTLQLAGLLAGASALGVVSPVLAHHSHAMFDHAKDVNITGTVV
                     DFAYVNPHGALDIAVDEKGQKVTYWIEMSNLTNMVQRKIKKATFKPGDKVSVKMHPLK
                     DGRPGGSYVTIVAADGTLYE"
     gene            13395..>14252
                     /locus_tag="VGM72_03335"
     CDS             13395..>14252
                     /locus_tag="VGM72_03335"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY4264324.1"
                     /translation="MRYPDAMGLSIRNLAMLGSEILGLDLSKPLAADDRAAIADALDT
                     QLVVVARDQRLSDPELIAFSRNFGELDPPGPNPYGEPFNKEFPEINVISNVIENGRPI
                     GGLGAGEAVWHADMTYTDVPPKAAILHALEIPADGGNTYFADMYAAYDTLPSGLKAAV
                     AGQTAVHDASRNSAGLLRKGYAEVRDVRETIGACHPLMRTDPESGRPALFLGRRYNSY
                     VVGLDVADSEALLDRLWDHATQTCFTMCHQWRAGDLLMWNNRAVLHRRDPFDENARRI
                     MHRTQIKGAN"
BASE COUNT         2798 a         4275 c         4135 g         3044 t
ORIGIN      
        1 caagacggtg cagttggatt tcggccagtc cttccgctat aacgagccgg tgctgcgcgt
       61 cattgccgaa cgcatgccgg tctcgatcta ttacgggatc gtgaccgcca tcttcacctt
      121 tgcgatctgc attccgcttg ggattctgaa agccattcgc caccgaagcg tggtggacaa
      181 tctgtcctcg gcgttgatct tcatcggcta tgcgattcca gggttcgccc tcggcgcggt
      241 tctggtcaat ctgttcgcgg tgcaattcca ggttttcccg ctggggggat tccagtccca
      301 aggcggcgag gcactgccgc ccgtggcgaa attcgtcgat atcgtctggc actcggtgtt
      361 gccgctggtg gcctacctcg ccggagcttt cgcgatcgag accatgctga tgaagaacag
      421 tctgatggag aatatgagcg ccgactatgt caggacggcg cttgccaagg ggctgacctg
      481 gcgccgcgcg gtgttcgtgc atgcgttgcg caactcgctg atccccatgg cgacgagtat
      541 cggaagcctg ctcgggattt tcctcacggg aagcgtgctg atcgaacgcg tcttcaacat
      601 ccagggtgtg ggtctcctgt tcttcgaagc gatccaggcg cgcgattttc ccgttgtgat
      661 gggtttcctg gtgatttcca gcgttatcct gatgctgggc aatctggtct cggacctcgc
      721 ggtcgccttt gtcgatccgc gcgtgcgatt cgagtagcac catgacgtgg atcaagctgg
      781 accccatcac gcggcgcagg tttgcccgat ttcgccagat caggcgtggt tattattcgt
      841 tcctgatcct gacggcggcg attctgcttt ccgtgttcgc gccgttcctg gcggagagcc
      901 gcgctctact ggttgtttac caggggcgcg ttttcctccc caccttccaa tatttgacga
      961 tggccatttt ccatcagacg ccgcctccgg gatggtcgag cgccgatctg gacaccgatt
     1021 acaatcggct gcagcacgaa tggcgggtgg agcgtttctt ctacgagcgc gaacgcgccg
     1081 aggccggaag cgatccggct aagctgtccg cgctggagac gaaatatccg gaccgccgca
     1141 gctacgtcat catgcctctg atcccgtggg acccgtatca gagcgatttc tggtacaatg
     1201 aaatcctcaa cgacattcag ggacttttga ccgcgggaaa tcgcgagggc gctgaacgcc
     1261 tggcacggca ggacgatctg gaagatctgg cgaatgcgat tcaaaccggg actatcgaca
     1321 ccatcctaaa ggacccgaaa cgctcgccat cgggcgatct cgccggtctt gcgcgcagcg
     1381 gaaccatgtc ctcgctcaag ggtctcgcac aagtgccgcc cacgggtccg gatttgcgcc
     1441 gcggccatta tttcggcacg gattcacagg ggcgcgatgt tgcctcgcgt ctgctctacg
     1501 gattccgcat ctcgattttc tttgcgcttt tcctcacgat ttccggacag gtaatcggca
     1561 ccatcatcgg cagcctgcaa ggttatctcg gcggcggctt cgatcttgca agccagcgcg
     1621 tcatcgaagt gctgatttcc atacccttcc tctatgtcgt gatcatcctc gccgccttga
     1681 tcgtgcccaa tttctggacg ctgcttctca tcatggcggt gttccagtgg atcgccatca
     1741 cgttttacat gcgcacggaa atgtatcgtg agaagaccaa ggaatattgc ctggcggcga
     1801 aatcctatgg tgcatcgcat atgcgcatca tcttccggca tctgttgccg aattgcttga
     1861 cgccgctggt gacgttcacg ccgttcgcga tcgtggcgtc gatcttcgcg cttacgggat
     1921 tggattatct cggctacggc ctgcccgcgc cgacgccgag ctggggagag atgatcgatc
     1981 aagcgcttcg tccggaaaac cgcgacaaat tatggctgac cttggcgcct ttcggagcga
     2041 tcaccatcac attggtgctg gtggtgttca tcggcgaatc ggtgcgtgaa gcgttcgatc
     2101 ccaaacgcta cgcgaaatat gaatgaaagg gttcgggcga tgaatctgac ccggcgttcc
     2161 gtggcgttga tggtgtcggc ggcggcctta ttccgcggac aactttcatg gccggcggaa
     2221 agcggcaaga tttcggctcc cgctccggcg gggcccgcgt tgctgcccaa ggatatcgtc
     2281 tgggaaaccg ccaatgacga gcctccgatc ggttcggaca aagcgatgcg cggcggcacg
     2341 ctcaatctgt cgatcagctc ctatcccctg acgttccgtc tttatggccc caacaacaac
     2401 gacagcttcg caacgtggaa tcgcgccttc accatcgggt tcagcctggt gcagatgcat
     2461 cccgtcaacg acaaatttct tccaatgctg gccacgcaat gggcggtgga gaaggatcag
     2521 aagacgctct atttcaagct cgacccggat gcgcgctggt ccgacggcaa acccatcacg
     2581 gccggcgatt acgtgttcgc ctggaaaatg ctgcagtcga aattcatcgt cgatcccttt
     2641 gcaaacacct acatgaagga atatttcgcg tccgtcgaca aaatcgacga ttacacactt
     2701 cgcatcgtcg gcacgcggcc gagctggcgt ccgctttacg attacaattt ttctccgatg
     2761 cccgcgcacg cgatcgtgct ggacgaaaat tgggtgacac gcaccaacaa ccaatttccg
     2821 gttgccgtcg gtccctatgt catctccggt gttgtgcgtg gagaatcggt gaccttcaag
     2881 cgtctcccga attggtgggg cgacaaaaag cgctatttca tcggcctgta caatttcgat
     2941 gagatccatt tgcgggtgat cccgacggac cgcgaactgg attatctccg ccagggcgaa
     3001 ctcgacatga tggctgaacc gtccgtcaag aattggcacg aggtctatac gtttcctgcc
     3061 gtgaccaacg gttggatccg gcgcgcgcgc gtctttgtcg acacgcccac cggcgtgaac
     3121 ggtttccaga tgaatctgga ggcgccggtc ttccagaaca aggatttccg cacggcgatg
     3181 caatatctgg tgaatttcga ccggctgaac aaaaatctct ggtacggcga atatttccgc
     3241 gtcaattctt tcttcgaagg gaccatcttc gccaatcccg acgtcaaggc gcggcccttc
     3301 gatccggcca aagcgcgcga atatctggag cgcgccggtt atcgccggcc cgacagcgtt
     3361 cgcaatcagg gcgtctgggc gcaattgcgc aatgtggcgt atgggctgct tttcacgcgc
     3421 tcggatacgg atgacatcct ggtcaacgat aagggcgaaa aagcgagctt tacgttgatg
     3481 tatctctaca aggcgctcga aaacgaaatg accgtgatcc agcaggattt tcgccgcgct
     3541 ggggtggaca tgcggctgca attgctggaa cccggggcgg cgttccagcg catgctggag
     3601 cggaaattcg agatgggctt cgtcaacatg acctcgggct attatcccga tccccggcaa
     3661 tatctgcata cggacttcaa gaacagcaag aacaacaatg atttttgggg atttgggacc
     3721 aaggaagtcg acgatctgat cggcgtttat gaaaaggatc tcgatcccaa ggcgcgcatg
     3781 gacgcgatgc atcgcatcga tcagattatc catgacgagg cgttcacgat tcctttccgt
     3841 acggcgccgt ttatccgcct tgtctattgg gactacgtgc agttcccgga tttctatctg
     3901 ccgctgcgta cgcagcaata tatggattac atggtctatt ggatcgatcc ggcgaagaag
     3961 gccgccttgg aagaggcgat gaagaccggc aagacctatc cggtcgatac gaacatggac
     4021 caggattatt acggcgtgcg caagaagttt cagtgagcgg gaataaacac cctgccaagg
     4081 tgagaagggg gcttcttggc gcttctggaa attgaaaatc ttcaggtcgg cttcgacacc
     4141 gaagcgggat tcgtgcatgc cgtcgacggg gtctcatgcc gtatcgattc ggggcgcacc
     4201 ttgggacttg tcggcgaatc gggttgcggc aaaagcgtga cggcaacgtc gatcctgcgc
     4261 cttgtcccaa atccgcccgg aaagatctat ggcggcgaaa tccggttcaa cggcaccgat
     4321 ctgctcaaac tgaaaagtga ggaactgccg aagattcgcg gcaaggagat cgccatgatc
     4381 ttccaggacc cgatgacctc gctcaatccg gtcttcacga tcgaaaagca gatgggcgaa
     4441 gtcctcaagc ttcgtttcgg catggaccgt cacgccgcgc gtcagcgttc cgtcgaaatg
     4501 ctcggcacgg tgggcatcgc cgatcccgcc tcgcgcctga ccaattatcc gcacgaactg
     4561 tccggcggca tgaagcagcg cgtgatgatc gccatggcgc ttctctgcga gcccaagctg
     4621 ctgatcgcgg acgagccgac caccgcgctt gacgtcacca ttcaggcgca gattcttcac
     4681 ctgatccggg aattgcagag gcgcacgggc gcggccgtcc tgttcatcac ccacgacatg
     4741 ggcgttgtcg cggagatgtg cgacgaagtg gccgtgatgt atgcgggccg cgtggtggag
     4801 cagggcgatg tcgtcgatat tttcaaacgc agccgtcatc cctacaccaa gggattgctc
     4861 cgcagcttgc cgcaaaaggg gcagcagaaa aaagccgagt tgccgaccat agagggcgtg
     4921 gtgccttcac tattggaccc gccgcaattc tgccgtttcg ccgaccgctg ctggaagcgc
     4981 aagcggctga gcgaagatct gcaaaaaaaa tgttggagcg aagacccgca attgcgcagc
     5041 gttgaaagcg gccttgtcgc ctgtcattac cccttggcgg gagaagagcc gtgagcgcgc
     5101 ccgtggatac gcctcttctg accgtcgaag gtctgaagaa atattatccg gtgcgcggcg
     5161 gggtgttcag cgcgaagatc ggggatgtgc gcgcggtcga cggagtcgat ctgaccgtgc
     5221 ggcgcggcga aacgctagga ctagtcggag aatccggttg cgggaaatcg acgctggggc
     5281 gcacgattct gcgcctggaa gaaccgaccg cgggccgcgt attgttcgag gggctcgata
     5341 tcgcgcgggc gtccaaatcc gaactcttca acctgcgccg caacatgcag gtgattttcc
     5401 aggatcccta ttcctcgctc aacccgcgca tgaccgtcgg cgagatcgtg cgcgagccgc
     5461 ttctggttca tcgcatcggc accaaagccg agcagatcga gaaagtgcgc gtgctgttgg
     5521 agaccgtcgg attgaccggc gaaatgctga atcgttatcc gcacgaattc tccggcggcc
     5581 aacgccaacg catcggcatc gcgcgcgcgc ttgcgctcga tccccaactc gtgatcgcgg
     5641 acgaaccggt ctccgcgctc gatgtctcgg tacgcagcca agtcctcaat ctcatggtcc
     5701 ggctgcagcg cgagcgcaat ctgacgtaca tcttcatcag tcacgacctg tcggtggtgg
     5761 aacatctcag cgacagcatc gccatcatgt atctcggccg catcgtggag aaggggccgg
     5821 cggaacgcgt cttcgcgaag cccgcgcacc cgtacacgcg cgcacttctg gaagcgattc
     5881 ccgtggccga tcccacgcgg cgccgtgaca gcatacccgt gcgcggcgaa gcgcccagtc
     5941 cggtgtcgcc gccgcccgga tgtccctttc atccgcgctg cgcttacgcc atcgatgcct
     6001 gccacaccat tgtgccgcct ctggagccgg tgggcattcc taacctcgtt ccgccgctcg
     6061 gccatgtggc cgcctgcatc cgcaagaacg aaatctgaaa aattcgttac ggcttcgccg
     6121 gcgcgacgtt ggccatcggc acataaccga tgccggtgcc attcttgccc acaagaaacc
     6181 agtcatagcc tttgggtctc gcaaggatgt ccaccggctg acccgcatcg aaatgcacgc
     6241 ccttgacctt gccgccccag atgaaggggc tgatgtagat gtcggtcgga cgcgccacag
     6301 tgtattttgc cgatgtcgcg tcataaccgt tcggcgcggg aaccacatcg tcctcgagag
     6361 gaatcgcatt gacggcggac ggctgggccg ccgccgaggc gctcatgcat gacatggcga
     6421 ggattgcggc cgcgatcgag gcgatgcagc tgccatgtct tgtgtgccgg atgatttttg
     6481 ttctcatggc tgatgcccga tgtcgcgaag gctcctcgcc gagctttatc gcgcagcgga
     6541 attctccgca acgtgcgcgg gatggttcgg cgtcacaatt ccggcggggg atgtcacaac
     6601 ctcttttcgt cttaatcgtt gtatagaatt tgcaggggcc aggatgaaga ctggaatgga
     6661 ggattctcat gaaacgcatc gcattgtttg ccgccacagc gctcgttttc gcaccatcgg
     6721 cggcaatcca agtccgggcg gccgacacca gccctggcaa agtctgcctg aatgcaagcc
     6781 agatccagcg caccgaagtt cctgacgaca ggaccatcat tttccacatg cgtgacggca
     6841 agatatggcg caacacgctg aagacggcgt gtccgatgct gaagatcagc ccatacaccc
     6901 agaagctgaa tggcgatctc gtctgttcca atcagcaatt catccatgtg gtgatgacgg
     6961 gaaacgattg cgtgctcggg gacttcacgc cgctcccgga cgaacgttga ggcgaggctg
     7021 tctttgccgc ccgtgccgat atcgggcaga atgactgaaa ccgtaagctt gagcgcgaaa
     7081 gccgataacg atgttcatga ccgcccatgt ccattcccgc cgcttgcttc gggcaagcct
     7141 ggccgccctc gtcctttgcg ccggcgcggt catgcccacg ctgcgggccc agccggtcgc
     7201 cggggtgagc ggcgcggtgc cggatttttc ttcgaacaga atgacgtggg tcctgatgaa
     7261 cggcacggcg ttcttccggg tgccgggtga cacaggcccg ggcccgatca tgcaacgggg
     7321 caaggagtac aagcatgacg atgtgccccg catcgccgaa accgataacc ccattctgaa
     7381 gccctgggcc aagaagctca tggacatcaa caatgcgcgc gttctggctg gcgatattcc
     7441 gttctacacc acgtcgcgct gttggcccgg cggtgttccg ggtcttttgc tctatcccgg
     7501 agagccggtg gttttcgccc agactcccaa ggaggtctgg attctctatc agcgcgatgc
     7561 ccaggtgcgg cggatttatc tgaatgtccc gcacagcaaa aatccggcct attcctggta
     7621 cggagaatcg gtgggccatt atgaaaatgg cgatacgctt gtcgtcgata ccatcggatt
     7681 ggacgataag gggccgatcg accgcttccg cacaccgcat accaagcaat tgcacgtggt
     7741 ggagcgctac cggctgaata atggcggcaa gaacatcgaa gtcacattca gcgtcgaaga
     7801 cccgggcgcg ttcaccatgc cttggagggc caaggtggat ttcgcgcgcg gccgtggagc
     7861 gcgctcggga gcctgggaag aatccatctg cgccgatggt ccggtcgatt atttcgccga
     7921 cagcattcca agcgcggttc ccattccgcg cgcggacaaa ccggatttct aaaccttagc
     7981 cgttactgtt gcgagatgcg ccgtgttgcg ccggcaacat tcttacgcat gctcaactgc
     8041 gacaaaaata cgccagcagt cagattctcg tgaactcgta ttgtttttcc cgcctgggaa
     8101 tgttgtcgtt aaaaaaaatt aacgccaaaa ccgggggaaa ttcatgaagt ccacgtcatt
     8161 acgttcgcac acatgcaatg cggttcgcct cgcggcattg ctcggcagcg catcttttat
     8221 ggccatggcc gcgactgagg ctgcttgggc gcaaggccaa gcgaccccgc cgcaaaatca
     8281 gacggctcaa gcgttggcgg ccatgcctcc ggagcaggtg ctcgtcaccg gatcgctgat
     8341 ccagggggct gcggcggtcg gcgtgccggt ctccgatctg aggcccaccg atttcgtgga
     8401 gacgggcaaa ctttccatca ccgaagttct taaatccgtt ccgtccctgc gcattgatgc
     8461 ggaagcatcg cccacttacg gcggcggtac gctcagcttc ctgcagaatg tgcagatcca
     8521 tggcctcggt acgggctcgg gcgtcgagac gctgcttctg gtcaatggcc ttcgctggcc
     8581 gccgcagaat tacagcaacg acaccgtcaa cccttcgatc attccccaga tcgcgcttga
     8641 gcgtatcgac gtgctgtcgg ccggtgcctc cgccgtctat ggctccgatg ccacggccgg
     8701 cgtgatcaac ctgatcctta aacgtggcta tgacggtgcg atgacccagg ccagcgtcac
     8761 cagctcgcca tcgaccggtt atctgcagga acaggtttcg cagctttatg gcaagtcatg
     8821 ggatagcggc aatatcaccg tcagctatgc attcaccgac agccgggcgg tgtcggcggg
     8881 gcggcgcagc tattacacgc aagatttcac gccatggggc ctgatggacc tcacgcccct
     8941 gggctcgagc attcccggca ttgcccatag cggcaatgcg gctacgcctg ccggtacgcc
     9001 ggcgctggaa accgcggcca gcgggaccat gttctgttcg aattgttttt ccatcccaaa
     9061 gggacaggac ggcaccggcc tgacgtgggc ggcaatcatg gccaatcccg gggtcaagaa
     9121 tgaacgcaat ccctggatag acagtgacgc caggccacgg ctgcaaacca acgccgtcac
     9181 cgggacgctc gatcaacagt tgaccaatga tttcttcgga cttggtccgg tcgcgctttt
     9241 tgccgatgga ttttattcca atcagcgcgg caagcagatt tatacgccgg gcaacggtca
     9301 ggcccgcaat gtacttgaga ccaacctgcc cataccggcc agcaatccat tccggccgac
     9361 cggcgcgccg gcaaatttgc gcgtcgatta cagcttcgct ctggaagcgc cgctgtatgt
     9421 cacgggcggt gaagtcgcca cccattggga cgccggattg aatctggaca agctgccttt
     9481 cgactggatc ggcaagctca cctattcgat gagcgacgac cgcaactatg gcgacgccac
     9541 caattcgatc aacaagaaca atctgctcgc ggcattgggc aacacggtga cgattcccgc
     9601 gacgagcacg gcgacctctt acagcttcgt caagccatcc gcggtacctt atctcaatgt
     9661 gttttgcgat ccgtcggcat tcacctgcaa cgatcctgca acgctggctt acatcgccgg
     9721 ctaccggctt cagcatgaga cgttcaagat ccaggagacc ggattcaatt tcaacgggcc
     9781 catcttcgat ctgcccggcg gaccggttaa actcgccttc gccggtcaga cgctcagcga
     9841 acactggacc tatctgaatc ttgagaacga caacaccgac agcacggccg tcgtccagaa
     9901 cgctcccgat gcggcttacc agagcagcta tgcgttcttc acccaggcag acattccgat
     9961 cttcggtcag gccaatgcta tccccttctt ccaatccctg gtcgtggagt tgggctatcg
    10021 ctatgacaaa tacaataatc tgaacgatcc tgtctggacg ccgaaggccg cagtgaactg
    10081 ggacgtcggc gcggggctca cgctgcgcgc ggcctggggc aaatccttcc gcgtgccgtc
    10141 cttcgccgaa aactcggtcg gcggatcgcg tgttgctggc tataacccgc tgggcggatt
    10201 ctcgaacgcg accaacgtga ccgtgctcga ttgcaaatcg gtcaacggct cgcccgccgg
    10261 cgtggcactt cccggatcgc tgacggccgt gctgaatccg acgtgttcca cggatccctc
    10321 gcttgtacag cctggcgggg tcagcgtgga actgagcggc aacggggccg ccgcggttct
    10381 gcgcggacac ggtctctcgc cgcagacgct aaatcagtgg agcaccggct tcaatttctc
    10441 gcccaatgac agcatcctgg gcggcgccct caacggcctg aatttcgata tctcttggtt
    10501 ccggctggag ttcaaaggtc tcatctccag caacggattg gggcagggtg gcaatgatcc
    10561 ggtctccttt gcacgctata cggtcattcc gcgtccggat ctgccgatca cggcgtcgga
    10621 gaatgcggcc ttcctcaatc tggtcaccgc actggccgcc gtgccgcagc ggtcgggctt
    10681 caccttcgat ccggccttta ttcccaacat caagttcatc caggacatcg cgctcaccaa
    10741 tatcggatcg cgggtgtttt ccggcatcga tttcgacgtc cgttacgatt tcgatctggg
    10801 ccgggtgggc ttgacggaca tgggcgcgct gaatatcggc gcggcgggtt tctacgagac
    10861 gatcgacaag agccgcgccg acgccggtca acctcttgtt tccgtgttcg agggaaaaga
    10921 ttccggcaat cgtctacagc gcgtgcgcta tcgccttggc tggagcgacg acacctggag
    10981 cgtcacggcc ttcgccaatt atttcggcca tggcgccaat acggcaacgc tgggcgtgaa
    11041 tttcgccggc aacaatctga tcccgccttg ctattattcc gcgggcttcg gacctggttc
    11101 ctgttatccc ggttcgccgt tctacggccc gtattcgacc tatcccaata tggcgcccgc
    11161 gatggtctat ttcgatctga cgatcggata tcagacgggt gaaaagccgg cgaatgaata
    11221 tctgcgcaat atcggcgtcc agttcaccgt caacgatatc ttcgaccggc cgccgccctt
    11281 cgaggttggt gcgcgcggca acgggtccat tcgcgccttc gagaattcgt tctccgatct
    11341 gcaacggacg ttcacgctga ccctgacaaa atcctggtaa ggcgtatcgc aaaagaaaag
    11401 agccgcgacc ggtttcggtg gcggctcttt tccgttgata tgtcccgatg cgcctatttc
    11461 tggccgggag gctttccgcc tgctgcaata tacgccttat accggcggtc gctttcggtc
    11521 agccacgtgc cccacgggcg ggaatccgcg ccgctccaat tgaagccagg atcgccggga
    11581 acgtgctcgt tcaaaagacc gttggcatcc atgaaaacct tcggtgaagg tccattggaa
    11641 tcgctgcaag tgttgtagag cggcctcttc tcgggcctat tgtccttgga cagcctgtaa
    11701 atgagccggg acgtgacggg cttcaccagc acctccggat cgtacagcgt gatttcgccc
    11761 tgaatctgat cgccaacgcg acgatatttc tccaccgttt ccagattgtc ggtgtattcc
    11821 tccaggccgc ctttccaggc cttcatctgg ttggtgtaga cgatcagcgt atcgccgttc
    11881 caaaagccga tcgactcacc ggcccatttg gggaattgca aatcttcggg ggtgtgaccg
    11941 ctgccgtcag tgtagatcca gcggatgccg ttccagccat tgccttccgt taaagtccac
    12001 acccggtcgg gcgtggcgat gaattcgcta gctgcgagcg tcggaaaaaa gccgttcggt
    12061 aggcagaacg cattcgccga ccaaatgcgc ccttcggtgg cagccttcaa ttcctgaacc
    12121 aaatattcct gatatttcgg cgtcagcaat tttacgacgt cgcttgccgg actgccgccg
    12181 cccccgaaac cgccgccgcc gccattccaa tgtccgctcc aatccgggat ggtggccttg
    12241 gtgtgcttga tgccgccatg ggcatcctta agccaagcct cgtaatgctc cttcgcggtc
    12301 ttgaacggat aggggctgcc gagattcgcg gcgcccgcct tgccggtgcc ttcggtgttg
    12361 tacacgcgcg aggagccgac ctgtccggta ttctcataag ggacaattcc gatcgccatg
    12421 ccgcgcagtt ggcccgccgt gttgttcttg taataggcag gatcggacca cagcgtgcga
    12481 tcctgatgga aatccttggt gttgaagatg gtggtttgtt cggccgcggg aacaggcagc
    12541 gacaagccca ccagcagcgc ggcgattgaa gccgtcgcca tgattttcgt attcacgatg
    12601 tatcctcccg tgccggtggg tcggccggcc attcgatcca agacctggcg aaaatctatt
    12661 cgtagagggt cccgtcggcg gcgacgatgg tgacgtagct gccacccggc cgaccatcct
    12721 tcaggggatg cattttgacc gacaccttgt cgccgggttt gaaggtcgcc tttttgattt
    12781 tgcgctgcac catgttcgtg agattcgaca tctcgatcca gtaggtcacc ttctggcctt
    12841 tttcatccac ggcgatatcg agcgccccgt gcggattgac gtaggcgaaa tccaccaccg
    12901 tcccggtgat attgacgtct ttggcgtgat cgaacatcgc atgggagtga tgcgccaaga
    12961 ccggcgaaac aacgcccaac gcgctcgcgc ccgccagcaa accggccaat tgcaacgtct
    13021 tcataactcg tcctccttat ggtcttgagg gccatatttc gctttcacga acgtcgcccc
    13081 tgccgttgtg attgccgtca aattagcaaa aaaaaattat cgcggccacg cattcgaatg
    13141 ccgcgcggga acagggatta agcttagcct aatcaatagc ttgctcagcc cggatttgac
    13201 gtaaccgggg gcaagcaagg ccccgcgtat cgaacgaccg aatcgcacca tgcggcccgc
    13261 gtgcccgatg cgaaattgct tctgatgcga aatggcttct tgtgtccgcc ggcattggac
    13321 cagccttgcg ctatgatttt cgcctggcgg ttcggccgct ttcgtctgaa cgctcgctgt
    13381 cagaagcgtg acgcatgcgc taccctgacg cgatggggct gtcgatcaga aatctcgcca
    13441 tgctcgggtc cgaaattctg ggtctcgatc tctccaaacc gctggctgcg gatgaccgcg
    13501 ctgccatcgc ggacgcattg gatacgcaac tggtcgtggt ggcgcgggac cagcggcttt
    13561 ccgatcccga actcatcgcc ttcagccgga atttcggtga actcgatccg cccggaccca
    13621 atccgtatgg cgagccgttc aacaaggaat ttcccgagat caatgtcatt tccaatgtga
    13681 tcgagaatgg ccgtccgatt ggcggtctcg gagcggggga ggcggtctgg cacgccgaca
    13741 tgacctatac ggatgtgccg cccaaagccg ccattctgca tgcgctggaa attcccgccg
    13801 atggcggcaa cacttatttc gccgacatgt acgcggccta tgacacgctg ccgtccgggc
    13861 ttaaggccgc cgtggccggt cagacggctg tgcacgatgc ttcgcgcaac agtgcgggac
    13921 ttttgcgcaa aggctacgcg gaggtgcgcg acgtgcgcga aaccattggc gcgtgccacc
    13981 cgctaatgcg caccgatccc gagagcgggc gcccagcgct gttcctcggc cggcgttaca
    14041 acagctatgt cgttggcctc gatgtggcgg acagcgaagc gttgctggac cggttgtggg
    14101 atcatgcgac gcagacatgt ttcaccatgt gccatcaatg gcgggccggc gatctcttga
    14161 tgtggaacaa tcgcgccgtg ctgcaccggc gcgacccctt cgacgagaat gcgcggcgca
    14221 tcatgcaccg tacacagatc aaaggcgcga at
//
LOCUS       DASYEG010000089         8012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_899062, whole genome shotgun sequence.
ACCESSION   DASYEG010000089 DASYEG010000000
VERSION     DASYEG010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 8012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8012
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899062"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..2470)
                     /locus_tag="VGM72_03340"
     CDS             complement(284..2470)
                     /locus_tag="VGM72_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY4264325.1"
                     /translation="MRTKRAACLGMMSASFAALLCAASANTAAAQGAAAGQVRVDADD
                     IGGVVTGAKGPEAGVWVIAETRDLPTKYVKIVATDDQGRYVIPDLPKGNYDIWVRGYG
                     LVDSPKVKTQPGKIVDLKSVAAPNRAAAAQYYPGVYWYAMMKIPPANLFPGTGPTGNG
                     MSVTLKDQGQWLRFTKTDGCGHCHQLGDLATRTLPADLGHFNTSAEAWERRLESGQAG
                     RNMMEELAKLDVQRQLNFLGDWTDRVAKGELPKSDPPRPVGMERNVVITEWDWETPTM
                     YVHDTISTDRRNPTVNGHGLIYGAPEVSSDNVPWLDPVDNKAGFIKSEYRDPKTPTTK
                     NDLILQPSPYWGNEPIWDSHTIIHNPMFDEKGRVWLTARIRPQANPAFCKEGSDQPSA
                     KIYPKNTSNRQVEVYDPKARKIDMIDLCFNTHHLQFDKNEVLWFSAGGNYDVIGWLDV
                     KKWEATHDDKASQGWSPFIIDTNGNGKRDASWTEPNQPNDAAKDKRMFLGFYGVAPNP
                     ADGTIWGGVTGFPGGIARFDPKTQLTEYYEFPYKNPKNPKSGFSPRGSDIDSNGVFWA
                     VLSSGDLASFDRRLCKGKLNGPAANDPQNICPEGFKLYQMPGPEFENLPKGTPGASAE
                     APYYVWVDQHNTFGLGENVPIATGNNSDSLNALVDGKWIVMRIPYPLSFYAKNLDGRI
                     DDMATGWKGRGLWTQYSGRSQPHLEGGKGQQSKVVHVQYRPDPLAK"
     gene            complement(2615..4801)
                     /locus_tag="VGM72_03345"
     CDS             complement(2615..4801)
                     /locus_tag="VGM72_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY4264326.1"
                     /translation="MRTRTASIGMLSTSAAALLCAVSMATLTAAPAAAQQVRVDADDI
                     GGVVTGAKGPEAGVWVIAETKDLPTKYVKIVVTDDQGRYLLPDLPKGNYDIWVRGYGL
                     VDSPKVKSAPGKTLDLKSVAAPSRAAAAEYYPAQYWYSMVKPPAATLFPGTGPQGNNM
                     SQQMTDQGVWFRNMKTDGCVACHQLGDKATRTIPAALGHFDTGAAAWERRLQSGQASG
                     PMMGAIGRFDTQRALAMFADWTDRVAKGELPKSDPPRPQGIERDIVVTEWDWNTPKAY
                     LHDEISTDRNNPSVNAHGIIYGSTEASSDYIPWLDPVDNKSGLLKTEYRDPKTPTTKN
                     DAIYGESPYWGNEPIWDSHTNVHNPMYDSQGRLWLTARIRPQANPAFCKEGSQQASAK
                     VFPQGRSGRQVEMFDPKTRKITMVDLCFSTHHLQFDKTGMLWFSSGGGNNEVVGWLDV
                     KKFDATHDEQASQGWSPFIIDTNGNGKRDAGWVEPNGKLDPTKDKRIITGLYSVSPNP
                     ADGSVWGTVLGFPGGVARFDPKTQLTEYFEVPWKNAKAEAMGFSPRGADITTDGVVWT
                     VLASGHFASFDRRLCKGKLSGPALADPQNLCPEGWKLYQMPGPEFEGLPKGTPGAGTE
                     APYYNWVDQYDTLGLGKNIPIATGNESDSLAAVVNGKWVVMRVPYPIGYFAKGMDGRI
                     DDPATGWKGKGLWSTFSDRAATHIEGGKGQMSKVVHFQLRPDPLAK"
     gene            complement(4995..7169)
                     /locus_tag="VGM72_03350"
     CDS             complement(4995..7169)
                     /locus_tag="VGM72_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264327.1"
                     /translation="MSTAHRSHLSMLTASLLGTVLFIASPLPASAQGAAPQVKADEIG
                     GVVTGAKGPEAGVWVIAETKDLPTKYVKIVVTDDQGRYLIPGLPKGSYDVWVRGYGLV
                     DSPRTKSALGKTVNLKATAAPNEAAAAQYYPAIYWYSMVKVPETNLFPGTGTSGNGMP
                     VSLTDQGMWLRYMKTDSCNSCHQIGDKATRELEPQLGHFETSSAAWERRLQSGQASAL
                     MMNGIGNFDTARALANLGDWTDRVKKGELPFAKPARPVGIERNVVLTEWDWSTPQTYL
                     HDEISTDRRNPTVNPHGLIYGSPEESSDFIPWLDPVDNKSGLIKSEYRDPKTPTTKTA
                     PQYAESPYWGSDAIWDSHTSIHNPWMDQKSRLWVTARIRPANAEPAFCKAGSKHPSAV
                     AFPLTRSGRQLEVYDPQTKKATPIDLCFSTHHLQLDKNDVMWFSSGNTNDEVVGWFDM
                     KKWDATHDEAASQGWAPFVVDTNGNGKVDDYVGPTDPVDPKKDKHVKVGFYGASPNPA
                     DGTVWASYLGFPGGVARFDPKTKLTEFYEVPYKDPKNPHSGFSPRGMDIDSNGVAWLA
                     LASGHMASFDRKLCKGPLNGPNAATGKACPEGWKLYTIPGPQFKGLAVADGSAEAPYY
                     DWVDQFDTLGLGKNVPIATGNESDALFALVDAKWVTMRVPYPMGFFAKNMDGRIDDAA
                     AGWKGKGIFSTYSGRSAPHIEGGKGQTSKVVHFQIRPNSLAD"
     gene            7382..>8012
                     /locus_tag="VGM72_03355"
     CDS             7382..>8012
                     /locus_tag="VGM72_03355"
                     /inference="COORDINATES: protein motif:HMM:NF036509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264328.1"
                     /translation="MAHLATHPGETKRDLARALGVKGPERQALKRILSSLADDGAIAR
                     GRRRSFVPAGELPEVTVLEIFGEDPDGEPLGRPVEWKNEGPAPSVLILPGREETGAPG
                     RGERVLARIARSHEGHAQYEARIIKRLGASAHRVLGVLKLETGMAPRIEPIDRKTRYA
                     FTIDPRELAGAKNGELVLVEPTAQHGAVGPRARIAERLGSMDEPKTVSLI"
BASE COUNT         1458 a         2453 c         2460 g         1641 t
ORIGIN      
        1 ggcggccgca tgtaggcgcg cgaataggaa taccacccgc ttgcggcggt cggcgcaaaa
       61 ttgggggcgg cgcttgcggc ggtcgccgtg aacaaggacg ttgcgagaag ggctgcggta
      121 aatcgggtca tggtccgctc gctgtgatgc cttcgcggcc aactatagca ccttgcaggc
      181 gctgtggcgc gagcggccat cccgaacgga gtccgccaac ccccaaaaga aaatggggcg
      241 gatatcatag gcatccaccc cattggctta tgctgcggtc aggttatttc gccagcggat
      301 cgggccggta ctgcacatgc acgaccttgc tctgctgccc cttgccgcct tcgagatgcg
      361 gctgggaacg gccggaatat tgggtccaca aaccgcggcc cttccagccc gtcgccatgt
      421 cgtcgatgcg cccgtcgaga ttcttggcat agaagctcag cggataggga atgcgcatca
      481 cgatccattt gccgtcgacg agcgcgttca gcgagtccga attgttgccg gtcgcgatgg
      541 gaacattctc gccgaggccg aacgtgttgt gctgatcgac ccagacataa tagggcgctt
      601 cggcagatgc gccgggcgtg cccttcggca ggttttcgaa ctcaggccca ggcatctggt
      661 aaagcttgaa gccttccgga cagatatttt gcggatcgtt ggccgccgga ccgttgagct
      721 tgcccttgca cagccggcgg tcaaagctcg ccagatcgcc gctcgacagc acggcccaga
      781 acacgccatt gctgtcgatg tccgaaccgc gcgggctgaa gcccgatttc ggattcttcg
      841 ggttcttgta cgggaactcg taatattccg tcagctgcgt cttggggtcg aaccgcgcaa
      901 tgccgcccgg gaagcccgtc acgccgcccc agatcgtgcc gtccgccgga ttgggcgcaa
      961 cgccatagaa gccgaggaac atgcgtttgt ccttggctgc atcattgggt tggtttggtt
     1021 cggtccagct tgcgtcgcgc ttgccattgc cattggtgtc gatgatgaag ggcgaccagc
     1081 cttggctcgc tttgtcgtcg tgggtggctt cccatttctt gacgtcgagc cagccgatga
     1141 catcgtaatt gccgccggcg ctgaaccaca gcacttcgtt cttgtcgaac tgcagatggt
     1201 gggtgttgaa gcacaaatcg atcatgtcga tcttgcgtgc tttcggatcg tagacttcca
     1261 cctgacggtt cgaggtgttc ttcggataaa tcttcgccga aggctgatcc gatccttcct
     1321 tgcagaacgc cggattggcc tgcgggcgaa tgcgcgccgt caaccacacg cgtcccttct
     1381 cgtcgaacat gggattgtga atgatggtgt gcgaatccca gatcggctca ttgccccaat
     1441 agggcgaagg ctgcaggatc agatcgttct tggtggtagg cgtcttcgga tcgcgatatt
     1501 ccgatttgat gaagccggct ttgttgtcca ccggatcgag ccagggaaca ttgtccgagc
     1561 tgacttccgg tgcgccatag atcaggccgt gaccgttgac ggtgggattg cgccgatccg
     1621 tcgaaatcgt gtcgtggaca tacattgtgg gggtttccca atcccactcc gtgatgacga
     1681 cattgcgttc catgccgacg ggacgcggcg gatcgctctt gggcaattcg ccctttgcga
     1741 cgcgatcagt ccaatcgcca aggaagttca gctggcgctg cacgtcgagc ttggcaagct
     1801 cttccatcat gttgcgtccg gcctgaccgg attcgaggcg gcgctcccag gcttcggcgg
     1861 atgtgttgaa atggccgaga tcggcgggaa gcgtgcgagt cgccagatcg cccaactgat
     1921 ggcaatggcc gcaaccgtcg gtcttggtga agcgcagcca ctggccctga tccttcaacg
     1981 ttacggacat gccgttgccc gtgggtcccg tgccggggaa cagattggcc ggcgggattt
     2041 tcatcatcgc gtaccaatag acgcccggat aatattgggc ggccgcggcg cgattgggcg
     2101 cagccacgga tttcagatcg acaattttgc cgggctgcgt cttcaccttc ggcgagtcga
     2161 cgaggccata gccacgcacc cagatgtcgt aattgccctt cggcagatcg gggatgacat
     2221 agcggccctg gtcgtcggtg gcgacgatct tgacgtattt ggtcggaaga tcgcgcgttt
     2281 cggcaatcac ccaaacgccg gcctcaggcc ccttggcgcc ggtgaccacg ccgccaatgt
     2341 cgtcggcatc gacccgcacc tgtccggcgg cggcaccttg cgcggctgcc gtatttgccg
     2401 acgcggcgca cagcagcgcc gcaaagctgg cgctcatcat gccgaggcag gctgctctct
     2461 tcgtcctcat tgtatcttcc ctcattgcgt taggagtgac gctgaaattc ttaggcggaa
     2521 cgagccggct ccggccgggc cggaggttac tcaaaaacga aggcggtttc caccagcggg
     2581 gaaccgcctt cgtctgacag accttgattg accgctattt tgcgagcgga tcgggacgca
     2641 gctggaagtg gacgaccttg ctcatctggc ccttgccgcc ttcgatatgc gtggcggcgc
     2701 ggtcggagaa cgtggaccac aggcccttgc ccttccagcc cgtggccgga tcgtcgatac
     2761 gtccgtccat gcccttggcg aaatagccaa tcgggtatgg cacgcgcatc acgacccatt
     2821 tgccattcac gacagccgcc agcgaatcgg attcatttcc ggtggcgatc ggaatgttct
     2881 tgccgagtcc gagcgtgtcg tattgatcga cccagttgta atagggcgct tcagtgccgg
     2941 cgcccggcgt gcccttgggc aggccttcga actcaggccc aggcatctga tacagcttcc
     3001 agccctcggg gcagaggttc tgcggatcgg ccagagccgg tccgctgagt ttgcccttgc
     3061 acagccggcg gtcgaagctg gcgaaatggc cgctcgccag aaccgtccac acgacgccat
     3121 cggtggtgat gtcggcgccg cgcggagaga agcccatcgc ttcggccttc gcgttcttcc
     3181 aaggcacctc gaaatattcg gtgagctgcg tcttgggatc gaagcgcgca acgccacccg
     3241 ggaagcccag caccgttccc cagaccgagc catcggccgg attcggagac acgctataga
     3301 ggccggtgat gatgcgcttg tccttggtcg gatcgagctt gccgttcggt tcgacccagc
     3361 cggcatcgcg cttgccgttg ccgttggtgt cgatgatgaa tggcgaccag ccttggctgg
     3421 cctgctcgtc atgcgtggca tcgaactttt tcacgtcgag ccaaccgacg acttcgttgt
     3481 tgccgccgcc cgagctgaac cacagcatgc ccgtcttgtc gaactgcaga tggtgcgtgc
     3541 tgaagcacag atcgaccatc gtgatcttgc gggttttcgg gtcgaacatc tccacctgac
     3601 gccctgacct tccttgcggg aagaccttgg cggaggcctg ctgcgaaccc tctttgcaga
     3661 aagcgggatt ggcttgcggg cgaatccgcg ccgtcagcca cagccggccc tggctgtcat
     3721 acatcgggtt atggacattg gtgtggctgt cccagatggg ttcattcccc caatagggcg
     3781 actcgccata gatcgcgtcg ttcttggtgg tcggcgtttt gggatcgcga tattcggtct
     3841 tcaagagacc ggatttgttg tccaccggat cgagccaggg aatgtaatcg gagctggctt
     3901 cggtcgatcc gtagatgatg ccatgcgcat tcacgctcgg attgttgcgg tcggtcgaaa
     3961 tctcatcatg cagataggcc ttgggcgtgt tccaatccca ttccgtaacc acgatatcgc
     4021 gctcgatacc ttgcggacgc ggcggatcgc tcttgggcaa ttcgcctttg gcgacgcggt
     4081 ccgtccaatc ggcgaacatc gcgagtgcac gctgcgtatc gaagcggcca atagcaccca
     4141 tcatggggcc gctcgcctgt cccgattgca gccggcgctc ccacgccgcc gcacccgtgt
     4201 cgaaatgacc gagcgccgcc ggaatggtgc gagtcgcctt gtcgcccaat tgatgacagg
     4261 cgacgcagcc atccgtcttc atgttgcgga accagacgcc ctgatcggtc atctgctggg
     4321 acatgttgtt gccttgcggg ccggtaccgg ggaacagagt ggctgccggc ggcttcacca
     4381 tcgaatacca atattgcgcc ggataatatt cggccgccgc cgcacggctc ggcgctgcaa
     4441 cggacttcag atcgagggtc ttgccggggg ccgatttcac tttcggcgaa tcgaccaggc
     4501 cgtagccgcg aacccagatg tcgtaattgc ccttcggcag gtcgggcagc agatagcggc
     4561 cctggtcgtc ggtcacgaca atcttcacat atttggtcgg cagatccttg gtttccgcga
     4621 tcacccaaac gccggcctcc gggccttttg cgcccgtgac gacgccgccg atatcgtcgg
     4681 catccacgcg tacttgctgg gctgcggcgg gggccgctgt gagcgtcgcc atcgacacgg
     4741 cgcatagcaa ggctgcggcg ctggtgctca gcatgccgat gctggctgtt ctcgtcctca
     4801 tctgttcgtc cctcattgcg ttaggattgg cttcaggcgt cggggtgaag tgcgatctta
     4861 tagcgcatcc gcaagcaaag aacgagggtg ctgcgacacg cgaaatcgtg aacccggaca
     4921 aacaaaaacg ggccccgttc gcacgggacc cgctcttaaa cgcagccctg gtgaaaccca
     4981 ggaccggaca attctcaatc cgccaaggaa ttcggacgga tctggaagtg cacgaccttg
     5041 ctggtctggc ccttgccacc ttcgatgtgc ggcgcggagc ggcccgaata ggtcgagaag
     5101 attcccttcc ccttccagcc ggcagcagcg tcatcaatgc ggccgtccat gttcttggcg
     5161 aagaagccca tcggatacgg aacgcgcatc gtcacccact ttgcgtcaac cagcgcgaac
     5221 agcgcgtcgg actcattgcc ggtggcgatc ggcacgttct tgccgagccc caacgtgtcg
     5281 aactgatcga cccaatcgta atagggcgct tcggcgctgc cgtccgccac ggccagaccc
     5341 ttaaactgcg ggccagggat ggtgtacagc ttccagcctt ccgggcaggc tttgccggtc
     5401 gccgcattgg gtccgttcag aggacctttg cagagcttgc ggtcaaagct cgccatgtgg
     5461 ccgctggcca acgccagcca ggcgacgcca ttgctgtcga tgtccatgcc gcgcgggctg
     5521 aagccggaat gcggattctt gggatccttg taggggacct catagaactc ggtcagcttc
     5581 gtcttgggat cgaagcgcgc cacgccaccg gggaatccga gataggaggc ccacacggtg
     5641 ccatccgccg gattcggcga agcaccatag aagccgacct tcacgtgctt gtccttcttc
     5701 ggatcgaccg gatcggtcgg gccgacataa tcgtcgacct tgccgttgcc gttggtgtcc
     5761 accacgaacg gcgcccaacc ttggctcgcc gcttcgtcat gggtggcatc ccatttcttc
     5821 atgtcgaacc agccaacaac ttcgtcattg gtgttgccgc tcgagaacca cataacgtcg
     5881 ttcttgtcga gttgcagatg gtgcgtcgag aagcagagat cgatcggcgt cgccttcttg
     5941 gtctgcggat cgtagacttc aagctgacgt cccgaacgcg tcaacgggaa tgctacagcc
     6001 gaaggatgct tcgaacccgc tttgcagaaa gccggctcgg cattcgccgg acgaatacgt
     6061 gcggtcaccc acagccggct cttctggtcc atccacggat tgtggatgct ggtgtggctg
     6121 tcccagatcg catccgaacc ccaatagggc gattccgcat attgcggcgc cgtcttggtg
     6181 gtgggcgttt tgggatcacg gtattccgat ttgatcaaac cggatttgtt gtccaccgga
     6241 tcgagccacg gaatgaagtc cgagctttct tccggcgagc catagatcag gccatgcgga
     6301 ttgactgtcg gattgcggcg gtcggtcgag atttcgtcat gcagataggt ctgcggggtc
     6361 gaccaatccc attccgtaag gacgacattg cgctcgatgc cgacgggacg cgccggcttg
     6421 gcgaagggaa gctcgccctt ctttacgcga tcggtccaat cgcccaaatt cgccaacgca
     6481 cgcgcggtat cgaaattgcc gatgccgttc atcatcagcg cactggcctg gccggattgc
     6541 agacggcgct cccaagcagc cgaggaggtt tcgaaatggc cgagttgcgg ctccagttca
     6601 cgcgtcgcct tgtcgccgat ctgatggcag gaattgcagc tgtcggtctt catgtagcgc
     6661 agccacatgc cctgatcggt gagcgaaacc ggcatgccat tgcccgacgt tccggtgccc
     6721 gggaacagat tggtttccgg caccttcacc atcgaatacc aatagatcgc aggataatat
     6781 tgcgcggcgg ccgcttcatt gggggccgcg gtggccttca ggttcacggt cttgccgagc
     6841 gcgctcttgg tccgcggtga gtcgacgagg ccatagccgc gcacccacac atcataactg
     6901 cccttcggca ggccggggat caaatagcgc ccctgatcgt ctgtcacgac gatcttcaca
     6961 tatttggtcg gcaggtcctt ggtttcggca atcacccaca cgccggcttc ggggcctttt
     7021 gcgcccgtga cgacgccgcc gatttcatct gccttcactt gcggtgcggc cccttgggcg
     7081 ctcgccggca gcggacttgc gataaacagg acggtgccca aaaggctcgc cgtcaacata
     7141 ctcagatgcg accgatgcgc ggtgctcatg ccccgtagtc ctccaaagct tgtagagttt
     7201 ggttatccaa ccctcggcaa tctagcggca atccgggccg cggcaaagcc gatcctgtcc
     7261 cagcgcgcga aaataagcgt tatgctcaag catccgtgaa cacgaggtcg cattggcaaa
     7321 aaagccccgc cgcacagacc gttctagcct ttcaaaaacc acgctgtcca aggaagccgt
     7381 gttggcccat cttgccaccc atccgggcga gaccaagcgc gatcttgccc gcgccctcgg
     7441 cgtgaaaggc ccggaacgcc aagcacttaa gaggattctc tcaagcctgg cagacgatgg
     7501 ggctatcgcg cgagggcgcc ggcgcagctt cgtgccggcg ggtgaacttc ccgaggtgac
     7561 ggtgctggag atcttcggtg aagaccccga tggagaacct ctcggccggc cggtggaatg
     7621 gaagaacgaa ggcccagccc cttcggtatt gatccttccg ggccgcgagg aaacgggggc
     7681 accggggcgc ggcgaacggg tattggcgcg aatcgcccgc agccatgagg gccatgctca
     7741 atacgaggcg cgcatcatca agcggctggg ggcgagcgcg catcgcgtgc tgggcgtgct
     7801 gaaactcgag accggcatgg cgccccgcat cgagcccatc gaccgcaaga cgcgctatgc
     7861 gttcacgatc gacccaaggg aattggccgg ggccaagaac ggcgaattgg tgctggtcga
     7921 accgaccgca cagcatggcg ccgtcggacc gcgcgcgcgc atcgccgagc ggttgggttc
     7981 gatggacgag cccaagaccg tcagcctgat cg
//
LOCUS       DASYEG010000090        23820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1333333, whole genome shotgun sequence.
ACCESSION   DASYEG010000090 DASYEG010000000
VERSION     DASYEG010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 23820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23820
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333333"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="VGM72_03360"
     CDS             <1..1118
                     /locus_tag="VGM72_03360"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY4264329.1"
                     /translation="NPVIVADRVGRTPAGVKHLVELAEALNAPVIDQLGRMNMPNNHY
                     LCNIGAGNLVANSDCVLGLELTDFWGTVNKMDDSATADYRPKIKPGTKLISIGVGDLY
                     IRANYQDFERYQPVDIAIGADAETALPMLTEYVKQAIPADRRAMIAARGDKAKKGQAA
                     ARERMLKAAADNAWDAQPISASRFSAELWNQIKGENWALVSRDSSPSNWPHRLWTFDQ
                     HHQFIGGPGGAGIGYGLPAAVGASFAHRAIDSSRLCINIQNDGDSMYAPGALWTASHH
                     KMPMLTVMWNNRAYHQEIMHLQRMANWRQRDVTTAHIGTTIWDPFVNYSKLAEALGQV
                     GIGPVTDPKDLAAAIKRGIEAVKMGEPVLIDVVCQPR"
     gene            1133..1525
                     /locus_tag="VGM72_03365"
     CDS             1133..1525
                     /locus_tag="VGM72_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY4264330.1"
                     /translation="MRNSIFIAASLMAACLFGASSAFAAAAAPQGDAAKGKANFERVG
                     CYQCHGHMGQGGREGPRIADPVPLAWPALQAWVRTTSGDMPPYTPKVLSDQELIDIYA
                     YLQSVPKSPDYKSIPLLSGMQNTAANAR"
     gene            complement(1537..2352)
                     /locus_tag="VGM72_03370"
     CDS             complement(1537..2352)
                     /locus_tag="VGM72_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HEY4264331.1"
                     /translation="MTVAVDLDAYFKRVGYGGSAEPTLETLRRLHALQPAAIAFENLD
                     SLMGRPVRIDAGAIQQKLISEGRGGYCYELNGLLGHVLRAIGFEVTGLLAHALLGQTM
                     AGTPRTHMVLKVETPEGPHIADVGFGSRTLSAPLSLEGAGEQTTPHGAFRLTRVGAYL
                     EEQALMEDAWTSLYRFSLEEHTQADYEVCNWYHSTHPASPFTNRLMASRFDGPRRLGL
                     LNNRFAIHHADGRTQTRMLASADEIAGVLEHQFAIVLPCPREALLATLARGLA"
     gene            2462..4153
                     /gene="ybaL"
                     /locus_tag="VGM72_03375"
     CDS             2462..4153
                     /gene="ybaL"
                     /locus_tag="VGM72_03375"
                     /inference="COORDINATES: protein motif:HMM:NF007950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HEY4264332.1"
                     /translation="MTQAPLISMLVIGFGLAFVFATIANRLKLSPIAGYLMAGVAVGP
                     FTPGFIGDPSLALQLADVGVILLMFGVGLHFSLHDLLALKKAVIPSVVAQVAFSASLG
                     GLVAWLSGFPIGAAAVFGVALSVASTVVLLRTLQNRRLLDTDRGRLAIGWLIVQDFVT
                     VIALVLIPAFAQLLKGQESGGTFDSTRLLETLALTFGKFVAFVVVMLVVGRRAIPAIL
                     HYVAHTGSRELFRLAVLAVALGVAFLAAALFGVSFALGAFFAGMILSESQLSARAAEE
                     SLPMRDAFAVLFFVSIGMLFNPMVLVHDALPLAACVVVVILGNGGIAFVLTRLLGYSV
                     ETSLLMAVGLAQIGEFSFILADLGIGLDLLTARARDLILGSSIISILANPFLVALASR
                     IHPVHPSGEPVKEDAPPELHPTTLAHHIVLVGYGRVGNIVGRALKERHEPFLVIEDAD
                     RLVAALHESAIEVIPGNAVRAEVLRAANVQEARLLFIAIPNGFEAGQIIQQSRALNPG
                     LRIIARAHSNDEVDYLLNLSADAVIMGEREIARGMMEHAFGESGVASTDTVALAT"
     gene            4198..5349
                     /locus_tag="VGM72_03380"
     CDS             4198..5349
                     /locus_tag="VGM72_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY4264333.1"
                     /translation="MQISASFRLLMGGAAAALAFVGICGPASADDSQRLLTIDHYVLT
                     HSIVPAIKGQPAQIYVREKARAATMARESGGANNVVLFVHGAGTPAEVAFDVPYQDYS
                     WMAYLARAGFDVFSMDTTGYGRSTRPAPMNDPCNLAPDQQPRLTPARCAATYPYQLTT
                     LESDWNDVGAVVDYLRSLRHVDRISLVGWSLGGPRSAGYAAQHPDKIARLVLLAPAYN
                     RDAKANPPGEIPFKGVAMNTQSRAEFDANWDRQVGCPDQYDPAASQSVWTNMLASDPV
                     GATWGTGVRRAPVTTSWGWTSDVVKKTTIPILMVSGAHDKQVPQQRVRDMYADLGSPQ
                     KIFIDLVCSSHNAMWEKNHRLLFQASLEWLQNGTVNGMKTGMLRFGYPD"
     gene            complement(5351..7693)
                     /locus_tag="VGM72_03385"
     CDS             complement(5351..7693)
                     /locus_tag="VGM72_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY4264334.1"
                     /translation="MPENGPSMSKHMAPASSQLHKEKDPVCGMDVDPGSARHSATHDG
                     QSYYFCCDGCRQKFLGEPARYLNGTPPPSALAPAGAIYTCPMHPEVRQIGPGSCPICG
                     MALEPVGVDMDSGPNPELAGMTRRLWAGAALSLPIVILEMGGHLPLLSAWVSAQASEW
                     LQAALAIPVVVWAGWPFLVRGWRSVVTSSLNMFTLIAMGTLVSLGYSLAAILAPSFFP
                     AAFRTSEGTIPVYFEAASVITVLVLLGQVLELRARERTGHAIRALLDLAPKTARRVRG
                     GSEKEIALDQVKVSDRLRVRPGEKIPVDGKVVDGSSSLDESMVTGESMPVGKTIGDSV
                     IGGTINQSGSLVIQAEKVGRDTMLARIVQMVAEAQRSRAPIQRLADRVSGWFVPGVLG
                     VAALAFVAWSFWGPEPRAVFGLVAAVSVLIVACPCALGLATPMSIMVGVGRGARAGIL
                     IKNAEALEHLEKIDVLVVDKTGTLTEGKPSLVRIGVAPGFEENELLRLAASAEQPSEH
                     PLARAVIAAAKARGLRLEQPADFLSHGGKGISGTAGGRKLVLGTSVFLAENHIDNSPL
                     GESADGLREEGATVLFGAIDGALAGIFAIADPIKPAAPEALGQLRAGNIRVVMLTGDN
                     RTTAEAVARRLGIADVEADILPEAKSAIVEKLRREGHVVAMAGDGINDAPALAAADVG
                     IAMGSGTDVAIESAGIALLKGDLTALARARALSRATMGNIRQNLFLAFVYNALGIPIA
                     AGILYPPFGILPSPIVAAAAMSLSSVSVIANALRLRMIRL"
     gene            7762..8037
                     /locus_tag="VGM72_03390"
     CDS             7762..8037
                     /locus_tag="VGM72_03390"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEY4264335.1"
                     /translation="MQDQAKTSCARRLSRIEGQVRGILRMIQEDRYCIDVMNQISAVQ
                     AALKRVEEEILRDHVAHCVEHAIASGDKIDQRRKVTELIEVLKHAGR"
     gene            8197..8865
                     /locus_tag="VGM72_03395"
     CDS             8197..8865
                     /locus_tag="VGM72_03395"
                     /inference="COORDINATES: protein motif:HMM:NF006184.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEY4264336.1"
                     /translation="MHIMEGFLPATHCIFWGAISAPFVIQGGRKLQRIMRDQPQMRLT
                     LAASGAFTFVLSALKMPSVTGSCSHPTGTGLGAVLFGPPVMAVLGAIVLLFQALLLAH
                     GGLTTLGANIFSMAIVGPWVSYFLYRLCRTMGLSIAVGVFVGAAFGDLATYLTTSVEL
                     AAAYPDAASGYVGAFLKFFGVFAMTQVPLAIAEGFLTVVVMNALSSKTHVLEELGLVR
                     GQMS"
     gene            8862..9185
                     /locus_tag="VGM72_03400"
     CDS             8862..9185
                     /locus_tag="VGM72_03400"
                     /inference="COORDINATES: protein motif:HMM:NF002780.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY4264337.1"
                     /translation="MIISSRRTNILLLLGVVVLVVVPLVFIKGEYGGSDDQGTAAIEA
                     LRPGYKPWFTPIWVPPSPEIESLLFALQAGLGAGVIGYVLGRIHGAVSARDAQKKSTP
                     GNVAH"
     gene            9172..9903
                     /gene="cbiQ"
                     /locus_tag="VGM72_03405"
     CDS             9172..9903
                     /gene="cbiQ"
                     /locus_tag="VGM72_03405"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HEY4264338.1"
                     /translation="MSHIDTSAHLNRWRKKSLAEKSLLAIGLLLIAVSVPSWHAAVLV
                     AAVMIGATLFGAGVAFSLWWKAMTAPIGFLFVGVFTLIFQVQFWHIGIAPHGLELAGR
                     VGARAFAGLTCLLFIALTTPATDLIGGLRRIGVPAEIAEMTLLIYRFVFLLTDTAETM
                     NAAQAARLGHITYQRHMKSLGLLIANLMPRAFARAQALEIGLAARGWHGELRVLSPVR
                     HASPLGLGIILALEAGVLLIGLWTS"
     gene            9900..10739
                     /locus_tag="VGM72_03410"
     CDS             9900..10739
                     /locus_tag="VGM72_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4264339.1"
                     /translation="MSLLEARALVHVYPGNVRALDGLNFRIERGKRLVILGPNGAGKT
                     TMLLHLNGTLRPQSGEVMLDGEVMGYAAASLTAWRRRVGLVLQNADDQLFAPSVAEDV
                     SFGPLNLGLDEAETRRRVTTALAAMHISHLAERSTHMLSGGQKRRVAIAGLVAMQPEI
                     LLLDEPTAGLDHEGTEDLLDVLEGLERDGTTLVFSTHDVELAYRFADEVAVFEGGRIL
                     AQGPIAATLTDTELVKRAKLGTPFLLKLGLRARELGILGPDEPMPRTPAAALRLLDLV
                     GVK"
     gene            complement(10743..11924)
                     /locus_tag="VGM72_03415"
     CDS             complement(10743..11924)
                     /locus_tag="VGM72_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264340.1"
                     /translation="MIFGPELLLVGAVAAVGVLHTIVPDHWVPITLIARQQAWSKGET
                     ARASLQAATGHVLSTLLIASVVWLAGVAFAERFGQIVNTVSSLALIAFGGWIAISAWR
                     ELHGGHGHGHSHAFPHLHGHGHGHEDGGHAASAHGPELQAIATDDGTIALSIFEAGVP
                     PRFRLTAPHADAFRVETRREDGGGELYMFANHGRYWESVEEIPEPHRFDVVVTVEHGG
                     HAHIYRTAFHEHDHHGHAEPEPAADDPLYAPSHGDTALLTRHAHAHRHGSVHAHWHDH
                     SADSSHPVTPDIETAPPAHVHRHKTSVRTALLLILGSSPMIEGIPAFFAAGQYGIGLI
                     VTMALVFAISTIATYMVLCVYSTAGLQRVRLGSFERYGEVLSGAFIAAIGAIFWIWPL
                     V"
     gene            complement(11921..12262)
                     /locus_tag="VGM72_03420"
     CDS             complement(11921..12262)
                     /locus_tag="VGM72_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY4264341.1"
                     /translation="MKQSRQTAAKRSSNDSTALAAHCARKLLDLRAHTQETQKQLARR
                     LRMTESMISRLERGDHVPSLKTLCRIADAFGRHLEIVFHEHEHAHADGTRHTHPHRHD
                     PDHPHAHGDDE"
     gene            complement(12464..13486)
                     /locus_tag="VGM72_03425"
     CDS             complement(12464..13486)
                     /locus_tag="VGM72_03425"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4264342.1"
                     /translation="MKKIDIFPHIFPKAYFDKMVEVIPNKAAVRRWLEIPVLYNLDAR
                     LKMMEEFGPDYQQILTLSMPAIEYVAPPETSPDMARLANDGMAEIVAKHKKQFPAFAA
                     SVPMNNVKAALAEIRRSVETLDARGIQIMTNVNGRALDDPELAPIFELMHSYDLPIWM
                     HPTRPQKFADYPNEGGSKYDIWWCFGWPYETSAAMARMVFSGFFDRWPNLKIITHHLG
                     AMVPFLEARVGLGLDQLGTREGAEPAYGEIVANMKKKNRRPVDYFRMFFADTAINGSK
                     SGTRCGIDFFGCDHVLFGSDCPFDPEGGPLFIRDIIKVLDEIDITEDERRKIYQLNAE
                     RMLKLK"
     gene            13612..15195
                     /locus_tag="VGM72_03430"
     CDS             13612..15195
                     /locus_tag="VGM72_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEY4264343.1"
                     /translation="MNATPPARGTPAAEAARRRTFAIISHPDAGKTTLTEQLLLLGGA
                     IRLAGAVKARGEARRARSDWMRIEQERGISVTTAVMTFEFEDTVFNLLDTPGHEDFSE
                     DTYRTLTAADSAVMVIDAAKGIEAQTRKLFEVCRLRDIPIITFINKMDREARDPFELV
                     DEIADQLQLECAPLNWPAGSGLNFRGVFDLEADDFVKFASGEPRTKDLSTLAEDERAK
                     LREDVELVRHASTPFDLETYRGGHMTPVFFGAALKNFGVRGLLQALARLAPPPRAQAA
                     QAREVQPDENQVTGFVFKVQANMDPNHRDRVAFLRLCSGRFQRGMKLKQSGTGKTIGV
                     HNPILFFAQEREVVDEAWPGDIIGIPNHGVLRVGDTLSESEDLVFTGIPNFAPEILRR
                     VRLFDAMKAKHLKRALESLAEEGVTQVFRPLLGAQWTVGVVGQLQLDVLKSRLRDEYS
                     LDVDLEASPYETARWVAGTPDEVAKFRDANRGQMAEDRDGGAVFLAKSAWELGYVGGK
                     FPEIRFLKTRERSYAAETA"
     gene            complement(15196..16395)
                     /locus_tag="VGM72_03435"
     CDS             complement(15196..16395)
                     /locus_tag="VGM72_03435"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264344.1"
                     /translation="MNIQPAPRSSGLDPVHPLADYPSFAALLEKTHPTLPYYTLHPAR
                     FREAASRFLQSFPGTTMYAVKANPALHVLDQIYAAGIRHFDTASLAEIALIRGRYPDS
                     TCHFMAPVRIPGEAGTAVREYGLTDFVVDCDPELDKLLGETDGGKGLRIFVRVATPLG
                     GAMLELSSKFGTTPVNAGRLLKRIASAGAKPALTFHVGSQCLSPFTYAQAVELARRAV
                     TAGGVTITALDVGGGFPGPYLGNDAPPSHWYFDTIREAIVDFPMEKDSDVFCEPGRAL
                     CAEGVSLAARVILRRDDQLFLNDGIYGSFDELSVPAWTADYPRRVYSFGPSGRVRERR
                     GELKPFKIFGPTCDTLDVLPRPLLLPQTIQQDDYIVFENMGAYSIALRTGFNGFYPND
                     WAIIDGE"
     gene            complement(16505..16924)
                     /locus_tag="VGM72_03440"
     CDS             complement(16505..16924)
                     /locus_tag="VGM72_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264345.1"
                     /translation="MRKSLVSLFLSSAMVVAPLAAAHAETSVACMYMLMRVYKAEMDA
                     CHVPLAADRDARYQRMRTNLEGFIRANAKNDPNRILSGVENDNIKRALAGLKSCKSDD
                     FKFARQAMDQITSAEHEKMIEGTLGIPRDPMSGDCSS"
     gene            complement(16995..17519)
                     /locus_tag="VGM72_03445"
     CDS             complement(16995..17519)
                     /locus_tag="VGM72_03445"
                     /inference="COORDINATES: protein motif:HMM:NF017849.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HEY4264346.1"
                     /translation="MLVKLEHGRARTVEDLFVAVGDDESLPHGAIVVPLKRFLAETTP
                     LLARETPLGVRLETGDTPQILGEHIHRLALIVLHVPYFKDGRGFSWARLARTRLGYTG
                     EIRVTGHFLKDQMAFYARVGVDVFDIAQNIPFAQIEAAFSEISNVYQPAFDGRATIRE
                     LRAAKRAAATISTS"
     gene            complement(17509..19164)
                     /locus_tag="VGM72_03450"
     CDS             complement(17509..19164)
                     /locus_tag="VGM72_03450"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005266030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEY4264347.1"
                     /translation="MYRYDEFDAQFVRERAEQFRGQVARRISGELTEDEFKPLRLMNG
                     LYLQLHAYMLRIAIPYGTLSARQLRKLAHLTRKYDRGYGHFTTRQNLQLHWAQLPRVP
                     DMLVELAEVEMHCIQTSGNSVRNISADPFAGAAAGELEDPRILSEILRQWSTLHPEFS
                     FLPRKFKFCVIASDEDRAALLIHDIAIKVIRNDSGELGYRVYVGGGLGRTPYLALEIG
                     SFVAKPDILAFCEAILRVYNEAGRRDNIHKARVKILVHQIGIEEMRRRVEAEFIEIKK
                     TGVLELPAEELARIGAYFADPPFETLPARCEAFEAAKRSHSDFADWVKTNVAPHKMPG
                     YAIATLSLKPIGKTPGDCSSEQMDAIAALMNTFGQAEIRVTHEQNLVLPHMRSSDLFA
                     LWQKLVPLELATANHGLVTDIIACPGLDYCDLANARSIPIAQAIAKRFADLERQHDLG
                     ELKIKISGCINACGHHHVGHIGILGVDRKGVENYQILLGGSGAEDASLAEIMGPGFDT
                     EGIVDAVENVIGTYTDMRQKGERFLDTYRRVGAVPFKERLYAR"
     gene            complement(19164..19499)
                     /locus_tag="VGM72_03455"
     CDS             complement(19164..19499)
                     /locus_tag="VGM72_03455"
                     /inference="COORDINATES: protein motif:HMM:NF022464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2849 domain-containing protein"
                     /protein_id="HEY4264348.1"
                     /translation="MQILTANRLKLGEVAYWNQGRWVSRLDEAELMSGAVAEAALKGA
                     GEFVRSGEIVAPYLFEAHETAGRTVPVKVREAVRAAGPTVRTDLGKQADLGKKTDLGE
                     KTGLGKKTG"
     gene            complement(19609..20403)
                     /locus_tag="VGM72_03460"
     CDS             complement(19609..20403)
                     /locus_tag="VGM72_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4264349.1"
                     /translation="MMNVSRTLRLLLPLALGVAVLALWEGLVAAYDIPRFVLPAPSAI
                     WAALIADFPSLMASLWTTLRITLLAFLLAVVSGVALAVFFSQSRIVEMALYPYAVILQ
                     VTPVVAIAPLIVIWVGFAHIDLALLILAWIVAFFPILSGTTLGLRSADHNLVDLFRLH
                     GASNWQILTRLRLPTALPFLLNAMKTSGGLALIGTVVAEYGAGSGTATGLAWRIVEAG
                     NRLQIATMFAALFLLAALGVMIFFALALVEYLVLRKWHESALKKDI"
     gene            complement(20400..21152)
                     /locus_tag="VGM72_03465"
     CDS             complement(20400..21152)
                     /locus_tag="VGM72_03465"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4264350.1"
                     /translation="MTIVASLRAVERVFSNGVKALGPLGLAIERGEFLSLLGPSGCGK
                     STSLRIIAGLLAPSAGTLDWPRTKPRIGFVFQDPTLMPWAIVRDNVRLPLDLAGVARA
                     DADAEAERALARVGLTGFSGAYPRVLSGGMRMRVSIARALAAKPQLLLMDEPFAALDE
                     ISRDALNEDLLKLWREDGLTIVFVTHSVYESTFLSTRIVTMTPRPGRVAQEFRLASPP
                     DRTSQWRMAPEFAEAARAVSRGLRDAAAKAAA"
     gene            21377..22225
                     /locus_tag="VGM72_03470"
     CDS             21377..22225
                     /locus_tag="VGM72_03470"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HEY4264351.1"
                     /translation="MAWADPIPEPVARIILKAAGSGDPATLQSVVDIAKATNPASVAE
                     IDALAARLRKEAEATRIAKLSSETFFEGWTGEGEAGASLTTGTSDNKTLALGVHLNKE
                     DLEWRHKIAAVANYQSSDDVTTSESYLASYEGNYRFAPRLYAFGLLQWEQDRFAGFNA
                     RYSESFGAGYTIVDTPAFNWQISTGPALRQTRRVTHEAESDASIRADTLLLWNLSPST
                     AFNEELGTYAGGSDSTYFSTTSLTTKIMGNLSARTSFNITSESNPPQGIDNTNTITRL
                     TLVYSF"
     gene            complement(22257..23297)
                     /locus_tag="VGM72_03475"
     CDS             complement(22257..23297)
                     /locus_tag="VGM72_03475"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4264352.1"
                     /translation="MRGLGRCLAAAALLAATACGPAETPKTPTPAKPQVHIGFVTDWK
                     AQAEHGGFYQALAEGLYARRGLDVTLLQGGAAVNVPQLLAGGAADFGMGSNNFIALNL
                     VREGAKIKAVMAVFQKDPQVLITHPRDDIKSIAGMKGKPIMIGDASTVTFWPWLKTKF
                     GFADSQIRKYTFNLAPFLVDKNAIQEGYLTSEPYTVEKEGKFTPQVFPLADAGYPGYA
                     NMVLVPEKWIAEHPDAVQAFVDASIEGWMHYLYGDPSAGNALIKRDNPDMTDDVLAQA
                     IDKMKTYGIALSGDAERLGLGAMTDARWQEFFDVMSKQNVYPANLDYRDAYTLQFIDK
                     GYALNDRHAAAK"
     gene            complement(23374..>23820)
                     /locus_tag="VGM72_03480"
     CDS             complement(23374..>23820)
                     /locus_tag="VGM72_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264353.1"
                     /translation="GVWVGNDDFSPMKKITGGSLPAQIWSGFMRTALKNSPHTHLPRA
                     EPVPPFIAEDGNTPQGGDNPIQRGLDGIGNFFDHLFGGGHANIASGPASKDISPPPET
                     SEARTHATPPAPPGETFVFENGETGERQVFQTGQGRPARQTPPLIP"
BASE COUNT         4460 a         7405 c         7546 g         4409 t
ORIGIN      
        1 acaatccggt catcgtcgcg gaccgcgtcg gacgcacgcc cgccggcgtc aaacatctgg
       61 tagaactcgc cgaagccttg aatgcgccgg tgatcgatca gctcggccgc atgaacatgc
      121 cgaacaatca ctatctctgt aatatcggcg ccggcaatct ggtcgccaat tcggactgcg
      181 tgctgggcct cgaactcacc gatttctggg gcacggtgaa caagatggac gacagcgcga
      241 ccgccgatta ccgcccgaag atcaaaccgg gcaccaagct gatcagcatc ggcgtcggcg
      301 atctctatat ccgcgccaat tatcaggatt tcgagcgcta tcagccggtg gacatcgcca
      361 tcggcgcgga cgccgaaacc gcgctgccca tgctgaccga atatgtgaaa caagcgatcc
      421 ccgccgaccg tcgcgccatg atcgcggcgc gcggcgacaa agcgaaaaag ggtcaagccg
      481 cggcacggga gcgcatgctg aaagccgccg ccgacaatgc ctgggacgcg caaccgatca
      541 gcgcgtcgcg cttctcggcc gagctatgga accagatcaa aggcgagaat tgggcgctgg
      601 tgtcgcgcga ttcgagcccg tcgaactggc cccatcgcct gtggaccttc gaccagcatc
      661 accaattcat cggcggcccg ggcggcgcgg gcatcggcta tggtcttccc gccgccgtgg
      721 gcgcgagctt cgcacatcgc gccatcgaca gttcgcggct ctgcatcaac atccagaacg
      781 acggcgattc catgtatgcg ccgggcgctt tgtggacggc ctcgcaccac aagatgccga
      841 tgctgaccgt catgtggaac aaccgcgcct atcaccagga gatcatgcat ctgcagcgca
      901 tggcgaactg gcggcagcgc gatgtgacca ccgcccatat cggcaccacg atctgggatc
      961 ccttcgtcaa ttactccaag ctcgccgaag ccttgggcca ggtcggcatc gggccggtga
     1021 ccgatcccaa ggatctcgca gccgccatca agcgcggcat cgaagcggtg aagatgggcg
     1081 aacccgtgct catcgacgtc gtctgtcagc cgcgttgaga ggagcgaggt ccatgcgcaa
     1141 ttccattttc atcgccgcgt ctctcatggc cgcgtgcctg ttcggcgcgt cttcggcctt
     1201 tgccgcagct gccgcccccc agggcgatgc ggccaagggc aaggccaatt tcgaacgcgt
     1261 cggctgctat caatgccacg gccatatggg acagggcggg cgcgagggcc cgcgcatcgc
     1321 cgatccggtg ccgctcgcct ggccggcact gcaagcctgg gttcggacga cgagcggcga
     1381 catgccgccc tatacgccca aggtgctgtc cgaccaggag ctgatcgata tctacgccta
     1441 tctgcaatcg gtgcccaaat caccggatta caaatcgatc ccccttctga gcgggatgca
     1501 gaacacggcc gcgaacgcgc gctgagcttt ttcgcgtcac gccagtcccc gcgcgagtgt
     1561 ggcaagcagc gcctcgcgcg ggcaaggcag cacaatcgca aattgatgtt cgagcacgcc
     1621 ggcgatttca tccgccgatg cgagcatgcg cgtttgggtg cggccatcgg cgtggtggat
     1681 cgcgaagcgg ttgttgagca ggcccagccg gcgcggaccg tcgaagcgcg atgccatcag
     1741 acggttggtg aagggcgatg ccggatgggt cgagtgatac cagttgcaga cctcgtaatc
     1801 ggcctgcgtg tgttcttcca gcgagaaacg gtagagactc gtccacgcgt cctccatgag
     1861 cgcctgctcc tccagatacg cgcccacgcg cgtcaggcgg aatgcgccat gcggcgtcgt
     1921 ctgctcgccc gcgccctcca ggcttaaggg ggcgctcaag gtgcggctgc caaatcccac
     1981 atcggcgata tgcggcccct ccggcgtctc gaccttgagg accatatggg tgcggggcgt
     2041 gcccgccatt gtctggccca gcaaggcatg ggccaaaagg ccggtgacct cgaaaccgat
     2101 tgcccgcagc acatgcccca ataggccgtt cagctcgtag caatagcccc cgcgtccctc
     2161 tgagatcagc ttctgctgga tcgcacccgc atcgatacga accggccgcc ccatcagcga
     2221 atccagattc tcgaatgcaa tagcggcggg ttgaagggcg tggagccggc gcagggtttc
     2281 aagcgtcggc tcggcagagc cgccataacc gacgcgttta aaataggcat cgagatcgac
     2341 ggcgacggtc acgcgattcc tccgagcctg gcggttttag aggaccccgc gcgtggtgac
     2401 caatcttaat cgcgcaaatc cccccttgcc gcgccaggcc gaacccgcga aagcttaagc
     2461 catgactcag gcgccgctca tttccatgct tgtcatcggg ttcgggctcg ccttcgtgtt
     2521 cgccaccatc gcgaaccggc tgaagctttc gccgattgcg ggctatctga tggcgggcgt
     2581 cgcggtcggc ccgttcacgc ctggattcat cggcgatccc agtctcgccc ttcaattggc
     2641 cgatgtcggc gtcattctcc tgatgttcgg cgtcgggctg catttctcgc tgcacgattt
     2701 gctcgcgctg aagaaagcgg tgatcccgag cgtggtggcg caggtcgcat tctccgcttc
     2761 gctcggcggg ctcgtcgcgt ggctcagcgg ctttcccatc ggcgcggccg ccgtcttcgg
     2821 cgtggccctc tcggttgcca gcaccgtcgt cctgctgcgc acgctccaga accggcggct
     2881 cctcgatacc gatcgtggcc gtctcgcgat cggctggttg atcgtccagg atttcgtcac
     2941 ggtcatcgcg ctggtcctga ttccggcctt cgcccaattg ttgaaggggc aggaaagcgg
     3001 cggcacgttc gacagcacgc ggcttctcga gacccttgcc ctgaccttcg gcaagttcgt
     3061 ggccttcgtc gtggtcatgc tggtggtcgg ccgccgtgcc attccggcaa tcctgcatta
     3121 tgtcgcccat accggctcgc gcgaattgtt ccgcctcgcg gtgcttgcgg tggcgctcgg
     3181 cgtggcgttc ctcgccgccg cattgttcgg cgtgtcgttc gcgctcggtg ccttcttcgc
     3241 cgggatgatc ttgagcgaat cccagctcag cgcccgcgcc gcggaagaat cgctgcccat
     3301 gcgcgatgcg tttgccgtgc tgtttttcgt atccatcggc atgctgttca atccgatggt
     3361 gctggtgcac gacgcgctgc cgctcgccgc gtgtgtcgtc gtcgtcattc tgggcaatgg
     3421 cgggatcgct ttcgttctga cgcgtctgct cggctattcc gtcgagacct cgcttctgat
     3481 ggcggtgggt ctcgcgcaga tcggcgaatt ttctttcatc ctcgccgatc tcggaatcgg
     3541 actcgatctc ctcaccgcgc gggcacgcga tctcatcctc ggttcgtcca tcatctcgat
     3601 tctcgcgaat ccattcctgg tggcgctcgc gagccgcatc catcccgtcc atccatccgg
     3661 cgagcccgtg aaagaggatg ccccgcccga attgcatccc acgacgcttg cccatcacat
     3721 cgttctcgtc ggctatggcc gcgtgggcaa cattgtcggt cgcgcgctga aggaacgcca
     3781 cgagcccttc ctcgtcatcg aagacgccga cagactggtg gccgctttgc acgagagcgc
     3841 gatcgaagtg ataccgggca acgcggttcg cgcggaggtt ctgagggcgg cgaatgtgca
     3901 ggaggcgagg cttctgttca tcgccatccc gaacggcttc gaagcgggcc agatcatcca
     3961 acaatcgcgt gctctgaatc cgggtcttcg aatcatcgcc cgcgcccatt ccaacgatga
     4021 ggtggactat ctgctgaatc tttccgccga cgccgtcatc atgggcgagc gcgagatcgc
     4081 gcgcggcatg atggagcacg ccttcggcga gtccggggtg gcatccaccg ataccgtggc
     4141 gctggccacc tgatccgcac ctcactagaa tgcgccgcgc taaaaggggt cacgcacatg
     4201 caaatctcgg catcgttccg gctgcttatg ggtggcgccg ccgcagcttt ggctttcgtg
     4261 ggcatctgcg gaccggcgag cgccgatgac agtcagcgtc ttctgaccat cgaccattac
     4321 gtcctcactc actccatcgt acccgcgatc aagggccagc ctgcgcagat ttatgtgcgc
     4381 gagaaggcgc gggcggccac catggcgcgc gaaagcggcg gcgccaacaa cgtcgtcctt
     4441 ttcgtgcacg gcgcgggaac gcctgcggaa gtcgcgttcg atgttccgta ccaggactat
     4501 agctggatgg cctatctcgc ccgcgcgggc ttcgacgtct tctcgatgga tacgacgggc
     4561 tatggccgct cgacgcggcc cgctcccatg aacgatccct gcaatctcgc gcccgaccag
     4621 cagccgaggc tgacgcccgc ccgttgcgcg gccacctatc cctatcagct caccacgctg
     4681 gaatccgatt ggaacgatgt cggcgcggta gtcgattatc tgaggtcgct caggcatgtc
     4741 gaccgcatca gcctggtggg atggtcgctc gggggcccgc gctccgcggg ctatgcggcc
     4801 cagcatccgg acaaaatcgc caggctggtt ctcttggcgc ccgcctacaa tcgcgatgcc
     4861 aaggcaaatc cgcccggcga aattccgttc aagggcgtgg ccatgaacac gcagtcgcgc
     4921 gcggagttcg acgccaattg ggaccggcag gtgggctgtc cggaccaata tgatccggcg
     4981 gcctcgcaat cggtgtggac caacatgctg gcctccgatc ccgtcggcgc gacatggggc
     5041 acgggtgtca ggcgcgcgcc ggtgacgacg agctggggct ggacctccga tgtcgtcaaa
     5101 aagacgacga tcccgatcct gatggtttcg ggggcgcatg acaagcaggt gccgcagcag
     5161 cgcgtgcgcg acatgtatgc cgatctcggt tcgccgcaga aaatcttcat cgacctggtc
     5221 tgctcgtctc acaacgcgat gtgggagaag aaccacagat tgctgttcca ggcttcgctc
     5281 gaatggctgc agaacggaac cgtgaacggc atgaagaccg gcatgctccg cttcggctat
     5341 ccggattgaa ttaaagcctg atcatccgca gccggagcgc gttggcgatg acgctgaccg
     5401 acgacagcga catcgccgcg gccgcgacga tcggcgaggg aagaatcccg aaaggcggat
     5461 agaggatacc ggcggcaatc ggtatgccca gcgcgttgta gacgaaggca agaaacagat
     5521 tctgccggat attgcccatt gtcgcgcggc taagcgcgcg cgcgcgcgcc agcgcggtca
     5581 gatcgccctt gagcaaggcg atgcccgcac tttcgatggc gacgtcggtg cccgatccca
     5641 tcgcaatgcc gacatcggcg gcggcaagcg cgggcgcatc gttgatcccg tcgccggcca
     5701 tcgccaccac atgaccctcg cgccggagct tctcgacgat ggcgctcttg gcctcgggca
     5761 ggatgtccgc ctcgacatcg gcaataccga gacggcgggc aacggcttcg gcggtcgtgc
     5821 gattgtcgcc cgtcagcatc acgacgcgga tgttgccggc gcgtagctgc ccgagcgctt
     5881 cgggcgcggc gggcttgatc ggatcggcaa tcgcgaagat tcccgccagc gcgccatcga
     5941 tggcgccaaa gagcaccgtt gctccttcct cgcgcagacc atcggcggat tcgccaagcg
     6001 gcgaattgtc gatgtgattt tcggcgagaa acacggacgt gcccagcacg agctttcgtc
     6061 cccccgccgt tcccgagatg cccttgccgc catggctgag aaaatccgcg ggctgttcga
     6121 ggcggagccc tcgcgccttg gccgcggcga tgaccgcccg cgcaagcgga tgttcgcttg
     6181 gctgctcggc gctcgccgcc aaacgcagca attcgttttc ttcgaaccct ggcgccacgc
     6241 cgatccgaac cagggaaggt ttgccttccg tcagcgtccc ggtcttgtcg acgaccagga
     6301 catcgatttt ttccaggtgc tcgagggctt cggcattctt gatgaggatc cccgcccgcg
     6361 cgccgcgccc tacccccacc atgatggaca tgggcgtcgc caagccgagc gcgcagggac
     6421 aggcgacgat cagaacggaa acggcggcga ccagaccgaa gaccgcgcgc ggctccggcc
     6481 cccaaaacga ccaggcaaca aaggcaagcg ccgcgacccc gagcacgccg ggaacgaacc
     6541 atcctgaaac gcggtccgcg agacgctgaa tgggggcgcg gctgcgctgt gcctcggcga
     6601 ccatctgcac aatgcgcgcg agcatcgtgt cgcgtcccac cttctccgcc tgaatcacga
     6661 ggctgccgct ttggttgatc gtgccgccaa tgacggagtc cccgatcgtc ttgccgacgg
     6721 gcatggattc tcccgtgacc atcgattcat ccagcgagga gctgccatcg acgaccttgc
     6781 cgtcgacagg gattttttct ccggggcgga cgcgcaagcg gtcgcttacc ttcacctgat
     6841 cgagcgcgat ttccttctcc gatccgccgc gcacgcggcg cgcggttttc ggcgccagat
     6901 cgagcagcgc gcggatcgca tgaccggtgc gctcgcgggc gcgcaactcc aggacctggc
     6961 ccagaagaac gagcacggtg atgaccgatg ccgcctcgaa atagaccggg atcgtgcctt
     7021 cggatgtgcg aaacgccgcg ggaaaaaagc tcggggccaa aattgcggca aggctgtagc
     7081 ccagcgagac taaggtcccc atggcgatca aggtgaacat gttgagggag gacgtcacaa
     7141 ccgaccgcca gccccgaacc aggaacggcc accccgccca gaccacgacg ggaattgcca
     7201 atgccgcctg aagccattcc gaggcctgcg cggacaccca tgccgaaagt aggggaagat
     7261 ggccgcccat ctccaggatg acgatgggaa gactcagcgc cgcgccggcc cacaatcgcc
     7321 gcgtcatgcc ggcaagctca ggattcggcc cggaatccat gtccacgccg acaggctcca
     7381 gcgccatgcc gcagatgggg cacgatcccg gcccgatctg ccgtacttcg ggatgcatcg
     7441 ggcaggtgta aatggcccct gccggagcca aggccgaagg tggcggcgtg ccgttcagat
     7501 agcgtgccgg ttcgcctagg aatttttgcc ggcagccgtc gcagcagaaa taataggatt
     7561 ggccgtcatg cgtcgcactg tgccgcgcgc tgccggggtc cacgtccatg ccgcagacag
     7621 gatccttttc tttgtgcaac tgcgaagagg ccggggccat gtgtttcgac atcgaagggc
     7681 cgttttccgg catggacatc cggtcttcct cacagcgata atcgcttgat aggtataccc
     7741 ccgtacggta tgtagcaagc catgcaagat caggcgaaaa catcctgtgc aagacggctt
     7801 tcgcgtatcg aaggccaggt ccgcggcatc ctgcgcatga tccaggagga ccggtattgc
     7861 atcgacgtca tgaatcagat ttcagcggtt caggcggctc tcaagcgcgt cgaggaggaa
     7921 atcctccgcg atcatgtggc gcactgcgtc gaacacgcca tcgccagtgg ggacaaaatc
     7981 gaccagcgcc gcaaagtgac tgagcttatt gaggttttaa aacatgccgg ccgctaagag
     8041 atcggaacat gaataatcgt ccattgtgtg attgccccag gggatggaag gacttgctgc
     8101 gtataggacg atagataaga tatggtctga acgggcgcga tatcaggggc acgcccgcca
     8161 tcgggaccag caacaggacg catctggagc ttaaccatgc acatcatgga aggttttttg
     8221 ccggccaccc actgcatatt ctggggcgcg atctcggctc cgttcgtaat ccagggcggc
     8281 aggaagctcc aacgcatcat gcgcgaccag ccgcagatgc ggctcactct ggcggcctcg
     8341 ggcgccttca cttttgtctt gtcggcgctg aagatgccga gcgtcaccgg ctcctgttcg
     8401 caccccacag gcacggggct cggcgcggtg ctgttcgggc cgccggtgat ggccgtgctc
     8461 ggcgcgatcg tcttgttgtt ccaggcgttg ctcctcgccc atggcggcct caccacattg
     8521 ggggcgaaca tcttctcgat ggcgatcgtc ggtccctggg tcagctattt tctgtatcga
     8581 ttatgccgga cgatgggcct ttccatcgcg gtgggggttt ttgtcggcgc ggccttcggc
     8641 gatctcgcca cctatttgac cacgtcggtc gaactcgcgg cggcctatcc cgacgcggcc
     8701 agcggttatg tcggcgcgtt ccttaaattc ttcggtgtct tcgcgatgac ccaggtgccg
     8761 cttgccatcg cggaagggtt cctgaccgtg gtggtgatga acgcgctttc ctccaagacg
     8821 catgtgctcg aagaactcgg cctggtgcgg gggcagatgt cgtgatcata agctcccgcc
     8881 gcaccaacat cctgctgtta ctcggggtcg tcgtattggt ggtggttcca ctcgtcttca
     8941 tcaaaggcga atatggcggc agcgatgacc agggtacggc cgctatcgaa gctcttcggc
     9001 cagggtataa gccctggttt acgcccattt gggtgccacc gagcccggag atagaaagcc
     9061 tgctcttcgc gcttcaagcg gggctcggcg cgggggtcat cggttatgtg ctgggccgca
     9121 tccatggcgc ggtcagcgcg cgcgacgcgc agaagaaatc cacgcccggc aatgtcgcac
     9181 attgacacca gcgcccacct caatcgttgg cgcaagaaat cgctggcgga aaaatccctg
     9241 ctcgcgatcg gcctgctgct gatcgcggtt tcggtgccat cctggcatgc cgccgttctg
     9301 gtggcggcgg tgatgatcgg ggcgacattg ttcggtgccg gcgtggcgtt ttcgctctgg
     9361 tggaaggcga tgaccgcgcc tatcggtttt ctgttcgtcg gcgtattcac gctcatcttc
     9421 caagtgcaat tctggcatat cgggattgcg ccgcatgggc ttgaattggc gggccgggtg
     9481 ggcgcgcgcg ccttcgccgg attgacctgc ctgctgttca tcgccctgac gacaccggcc
     9541 acggatctga tcgggggctt gcgccgcatc ggcgtgccgg cggagatcgc cgagatgacg
     9601 cttctgatct accgcttcgt attcctgctc accgatacgg cggagactat gaacgccgcg
     9661 caggccgccc ggctcggtca catcacctat cagcgtcaca tgaaatcgct cggtctcctg
     9721 atcgccaatt tgatgccgcg ggccttcgcc cgggcgcagg ctctggagat cggtctcgcg
     9781 gcgcgcggct ggcacggcga gctgcgggtt ctaagccccg tgaggcacgc ttccccgctt
     9841 gggctcggga tcattcttgc gctcgaagca ggcgttcttc tgatcggatt gtggacctcg
     9901 tgagcctgct cgaagcgcgt gcgctcgtcc acgtctatcc cggtaatgtg cgggcgctcg
     9961 acgggctgaa cttccgcatc gagcgcggaa aacgcctggt gatcctcggc ccgaacggtg
    10021 cgggcaaaac caccatgctg ctgcatctca acggcacgct ccgtccgcaa tcgggcgagg
    10081 tgatgctcga tggtgaggtg atggggtacg cggcagcctc cctcaccgcg tggcgccgcc
    10141 gggtgggact tgtgctgcag aacgccgatg atcagctctt cgcgccttct gtcgcggagg
    10201 atgtgtcgtt cggtccgctc aatctggggc tcgacgaggc cgagacccgc cggcgcgtga
    10261 ccacagcgct cgccgcgatg catatttcgc atctggcgga gcgctcgacc catatgctgt
    10321 ccggcggcca gaagcggcgc gtggccatcg ccgggctggt ggccatgcag ccggagatcc
    10381 tgctgctgga cgagcccacg gccgggctcg atcatgaggg caccgaagac cttctggacg
    10441 tgttggaagg cttggaacgc gacggcacga cgcttgtgtt cagcacccat gatgtggagc
    10501 tggcttatcg ctttgccgat gaggtcgcgg tgttcgaggg cgggcggatt ctggcgcagg
    10561 gtccgattgc cgcgacgctc accgataccg aactggtgaa gcgcgccaag ctcggcacgc
    10621 cgtttctgct gaagctgggc ctgcgggcgc gcgaattggg aattctgggg cctgacgaac
    10681 ctatgccgcg tacgcccgcc gccgcactca gattgctcga tctggtcggc gtcaaatagc
    10741 cgtcacacca gcggccaaat ccagaatatc gcgccgatag cggcaatgaa ggcgccactc
    10801 agcacctcgc catagcgttc gaacgaaccc aaacgcacgc gctgaagtcc ggccgtcgag
    10861 tacacgcaca acaccatata ggtggcgatg gtgctgatgg cgaagacaag cgccatcgtg
    10921 acgatgaggc cgatgccata ttggccggcg gcaaagaatg ccggaatgcc ttcgatcatc
    10981 ggtgacgagc ccagaatgag cagcaacgcg gtgcgcacgc tcgtcttgtg gcgatggaca
    11041 tgggcgggcg gcgcggtctc gatgtccggc gtaaccggat gcgaggaatc cgcgctgtga
    11101 tcgtgccaat gcgcgtgcac cgatccgtga cggtgggcat gggcatggcg cgtgagcaaa
    11161 gccgtgtcgc catgcgacgg cgcatagagc ggatcgtccg ccgccggctc cggctccgca
    11221 tgcccgtgat gatcatgctc atggaacgcc gtccgataga tgtgcgcgtg gccgccatgc
    11281 tcgaccgtca ccaccacatc gaaccgatgc ggttcgggaa tctcttcgac cgattcccaa
    11341 tatctgccgt gattggcgaa catatacagc tcgccgccac cgtcttcccg ccgcgtctcg
    11401 acgcgaaatg catccgcatg gggtgcggtg aggcggaagc gcggcggcac gcccgcctcg
    11461 aagatcgaca gcgcgatggt gccgtcatcc gtcgcgattg cctggagctc cggcccatgg
    11521 gcggaagcgg cgtgtccgcc atcctcgtga ccatgaccat gaccgtgcaa atgcggaaac
    11581 gcgtggctgt gcccgtgtcc atgcccgccg tgcagctccc gccacgccga aatcgcgatc
    11641 cagccgccga acgcgatcag cgccaggctg gacaccgtgt tgacgatctg gccgaagcgc
    11701 tccgcgaacg cgacaccggc cagccacacg acggaggcga tcaacagagt cgaaagcaca
    11761 tgtccggtcg cggcctgcaa cgaggcacgc gccgtttcgc ccttcgacca cgcctgctgc
    11821 cgcgcgatca gcgtgatcgg cacccaatga tccggcacga tggtgtgcag cacgccgacc
    11881 gccgcgacgg cgccgaccag aagcagctcg ggtccgaaga tcattcatcg tctccatgcg
    11941 cgtgcgggtg atcgggatcg tggcgatgcg gatgggtgtg gcgggtgccg tcggcatggg
    12001 cgtgttcatg ttcgtggaac acgatctcca gatgccggcc gaacgcatcg gcgatccggc
    12061 acagcgtctt gaggctcgga acatgatcgc cgcgctccag gcgcgagatc atcgattcgg
    12121 tcatgcgcag gcgccgcgcg agctgctttt gcgtctcctg cgtatgggcg cgcagatcga
    12181 gcagcttgcg cgcgcaatgg gcggcaagcg ccgtgctgtc gttgctgctg cgcttggcgg
    12241 ctgtctggcg ggattgtttc atatcatccc tcaacatatg gggaggcgaa gccctatcgg
    12301 ggacttcgca aatttgcgaa ggtctttact tagcaaatct gcgaagttcg cgcaaccaca
    12361 ggtaaagacc tcttaaacgg cgtctcgagg tcgcaactta agacgtgcgt catcttcggg
    12421 atggggagac gcggcaggcg atatcgccgt gaccggcccg tgattatttc agcttcagca
    12481 tccgctccgc attgagctga tagatcttgc gccgctcgtc ctcggtgatg tcgatctcat
    12541 cgagcacctt gatgatgtcg cggatgaaca gcggtccgcc ttcgggatcg aacgggcaat
    12601 cgctgccgaa cagcacgtga tcgcagccga agaaatcgat gccgcagcgc gtccccgatt
    12661 tggagccgtt gatggcggtg tcggcgaaga acatgcggaa ataatccacc ggccggcggt
    12721 tctttttctt catgttggcg acgatctcgc cataggccgg ttccgcgcct tcgcgcgtgc
    12781 cgagctgatc caggccaaga ccgacacgcg cttccaggaa cggaaccatc gcgcccagat
    12841 gatgggtgat gatcttcaaa ttcggccagc ggtcgaagaa gcccgagaac accatccgcg
    12901 ccatggcggc gctggtttca tagggccagc caaagcacca ccagatgtcg tatttggaac
    12961 cgccctcgtt cgggtagtcg gcgaatttct gcggccgcgt cggatgcatc caaatcggca
    13021 gatcgtagga atgcatcagc tcgaagatgg gcgcgagttc gggatcgtcc agcgcgcgcc
    13081 cgttgacgtt ggtcatgatc tggatgccgc gcgcatccag cgtctccacg ctgcgccgga
    13141 tttcggcgag tgccgccttg acattgttca tcggcaccga ggccgcgaag gccgggaact
    13201 gcttcttatg tttggcgacg atctcggcca tgccgtcatt ggcgagcctg gccatgtccg
    13261 gggaggtctc cggcggcgcc acatattcga tggccggcat ggaaagggtg agtatctgct
    13321 gatagtccgg accgaactcc tccatcatct tcagacgcgc atccagattg tagagcaccg
    13381 ggatctcgag ccagcgccgc accgcggctt tgttcgggat cacttcgacc attttgtcga
    13441 aataggcctt gggaaagatg tgcggaaaaa tgtcgatttt cttcacggga gcaaggcctt
    13501 tcggatgcgg gaaatggcag cgcgcgggac cttagaccgg cccgttccca ggcggcaagc
    13561 gttgcatcgc ccaaacgcca ctgctagcac cttcgcaagc caaggatttc catgaacgcc
    13621 acgccacctg caagaggaac gcccgccgcc gaggcggcaa gacggcgcac tttcgcgatc
    13681 atctcgcacc ccgacgcggg caagacgacg ctgaccgagc aattgctgct tctgggcggc
    13741 gcgatccggc tggccggcgc ggtgaaggcg aggggcgagg cgcggcgcgc gcgctcggac
    13801 tggatgcgga tcgagcagga acgcggcatc tcggtcacca ccgcggtgat gacgttcgag
    13861 ttcgaggaca cggtgttcaa tcttctcgac acgccgggcc atgaggattt ctcggaagac
    13921 acctatcgca cactgacggc ggcggattcg gcggtgatgg tgatcgatgc cgccaagggc
    13981 atcgaggccc agacgcgcaa attgttcgag gtctgccgcc tgcgcgacat ccccatcatc
    14041 accttcatca acaagatgga ccgggaggcg cgcgatccct tcgagcttgt cgacgagatc
    14101 gccgaccagc tccagctcga atgtgcgccc ctgaactggc cggccggatc ggggctgaat
    14161 tttcgcggcg tgttcgatct ggaggcagat gatttcgtca aattcgccag cggtgaaccc
    14221 agaacgaagg atttgagcac gctcgcggag gacgagcggg cgaagctgcg cgaagatgtc
    14281 gagctggtgc gccatgcgtc cacgcccttc gatctcgaaa cctatcgcgg cggccacatg
    14341 acgccggtgt tcttcggcgc ggcgctgaag aattttggcg tccgcgggct tttgcaggcg
    14401 ctcgcgcggc tggcgccgcc gccgcgcgcc caggccgctc aggcgcgtga ggttcagccg
    14461 gacgaaaatc aggtcaccgg gtttgtcttc aaggtccagg ccaatatgga ccccaaccat
    14521 cgcgaccggg tggcgttcct gcgtctctgt tcggggcgct ttcagcgcgg catgaagctg
    14581 aagcaatcgg gcaccggcaa gaccatcggc gtgcacaatc cgattctctt tttcgcccag
    14641 gagcgcgaag tggtggatga ggcgtggccc ggcgacatca tcggcattcc caatcatggc
    14701 gtgctgcgcg tcggcgacac gctgagcgag agcgaagacc tcgtcttcac cggcatcccc
    14761 aatttcgcgc cggaaatcct gcgccgcgtg cgcctgttcg acgcgatgaa ggccaagcat
    14821 ctgaagcgtg cgctggaaag cctcgccgag gaaggcgtga cgcaggtgtt caggccgctg
    14881 ttgggcgcgc aatggacggt gggggtcgtc ggtcaattgc agctcgacgt gctgaaaagc
    14941 cggctgcgcg atgaatatag tctggatgtg gatttggaag catcgcctta cgagacggcg
    15001 cgctgggtgg cgggcacgcc cgacgaggtg gcgaaattcc gcgatgccaa tcgcggccag
    15061 atggcggagg atcgcgacgg gggcgcggta ttcctggcga agagcgcctg ggaattgggc
    15121 tatgtcggcg ggaaatttcc ggagatccgg ttcttgaaga cgcgcgaacg cagctacgcc
    15181 gccgaaacgg cgtaatcatt ccccgtcgat gatcgcccaa tcattggggt agaagccgtt
    15241 gaagccggtg cgcagcgcga tggaataggc gcccatattt tcgaagacga tataatcgtc
    15301 ctgctggatc gtttgcggca ggaggagcgg acgcggcagc acgtccagcg tgtcgcaggt
    15361 ggggccgaaa atcttgaacg gcttcagctc gccgcgccgt tcgcgcacgc gtccgcttgg
    15421 cccgaaggaa tagacgcggc gcggataatc cgcggtccag gcagggacgg agagttcatc
    15481 gaagctgcca tagatgccgt cattcaggaa tagctggtcg tcgcggcgca ggatcacgcg
    15541 cgcggccaac gacacgcctt ccgcgcacag cgcccggccg ggctcgcaga agacatcgct
    15601 gtctttttcc atgggaaaat ccacaatcgc ctcgcggatg gtgtcgaaat accaatgcga
    15661 gggcggcgcg tcattgccca aataagggcc gggaaaaccg cctccgacat cgagcgcggt
    15721 aatcgtaacg ccgcccgccg tcaccgcgcg gcgcgccagc tccacggcct gggcataggt
    15781 gaaaggagaa aggcattgcg agccaacatg gaaggtgagg gccggtttcg cgcccgcgct
    15841 cgcgatgcgc ttcaaaagac gcccggcatt aaccggcgtg gtgccgaatt tgctggacag
    15901 ctccagcatg gcgccgccca ggggcgtggc cacgcgcacg aagatgcgca agccctttcc
    15961 gccgtcggtt tcgccaagca gcttgtcgag ttccggatcg caatcgacca cgaaatcggt
    16021 cagcccatac tcgcgcacgg cggtcccggc ctcgcccgga atgcgcaccg gcgccatgaa
    16081 atggcaggtc gaatcgggat agcggccgcg gatcagcgcg atctcggcca aggaagcggt
    16141 gtcgaaatgg cggatgccgg ccgcataaat ctggtcgagc acgtgcaagg ccggattggc
    16201 cttgaccgca tacatcgtgg tgccggggaa ggattgcagg aaccgacttg ccgcctcgcg
    16261 gaaacgcgct gggtgcagcg tgtaataggg cagcgtcgga tgggtctttt ccagcagcgc
    16321 ggcgaaggaa ggatagtccg cgagcgggtg gacaggatcg agtccggaag atcggggcgc
    16381 cggctggata ttcatggacg cgttacccca aagaaggcgc gctttttagg cgcacggggg
    16441 acgagggtca agcaatacgg cgcagcgggg gccggacggc gcagcgggag gctggcattc
    16501 acgatcaact gctgcaatcg ccggacatcg gatcgcgcgg aatgcctaat gtgccctcga
    16561 tcatcttctc gtgctcggcc gaggtgatct gatccatggc ctgccgcgcg aatttgaaat
    16621 cgtccgattt gcaggatttc aagccggcca aagcgcgttt gatgttgtcg ttctcgacac
    16681 cggaaaggat ccggttgggg tcgttcttgg cgttcgcgcg aatgaagcct tcgagattcg
    16741 tccgcatgcg ctgatagcgg gcgtcgcgat ccgccgccag gggcacgtga caggcatcca
    16801 tctccgcctt gtagacgcgc atcagcatgt acatgcaggc caccgaggtt tccgcatgag
    16861 ccgccgccaa cggcgccacc accatcgccg aggacagaaa caacgacaca aggcttttgc
    16921 gcatggatgc gcctccagga aaggccacag aaaataacgt cagaggccca taaaaggttc
    16981 gcgaagttaa caattcaaga ggtcgaaatt gtggctgcgg cccgcttggc ggcacgcaat
    17041 tcgcggatgg tggcacgtcc atcgaaagcg ggttgataga cattgctgat ttccgaaaac
    17101 gcggcctcga tctgcgcaaa gggaatgttc tgtgcgatgt cgaagacgtc cacgccgacg
    17161 cgcgcataga atgccatctg atctttcagg aaatggccgg tgacccggat ttcgccggtg
    17221 tagccgagcc gggtacgggc aagacgcgcc caggaaaatc cgcgtccatc cttgaaataa
    17281 ggcacatgca ggacgatgag cgcgagcctg tggatgtgct cgccgaggat ttgcggggtg
    17341 tcgcccgtct ccagccgaac accaagcggc gtctcacgcg ccagaagcgg cgttgtctcg
    17401 gccaggaagc gcttcagcgg cacgacgatg gcgccgtgcg gcagggattc gtcatcgcca
    17461 accgcgacga acaggtcctc gaccgtgcgt gcccgcccgt gttcgagctt aacgagcata
    17521 tagacgctcc ttgaagggaa cagccccgac gcggcgataa gtgtcgagga agcgttcccc
    17581 cttctggcgc atatccgtat aggtgccgat cacgttttcc acggcgtcca cgatgccttc
    17641 ggtgtcgaag ccgggaccca tgatttcggc caggctcgca tcttccgcgc cggagcctcc
    17701 aagcaggatc tggtaattct cgaccccctt ccgatccacg cccaatatgc cgatatggcc
    17761 gacatgatga tgaccgcagg cattgatgca gccggaaatc ttgatcttca gctcacccag
    17821 atcgtgttgg cgttcaagat cggcgaaacg cttggcgatc gcctgcgcga tggggatgga
    17881 acgcgcattg gcaagatcgc aataatccag gccggggcag gcgatgatgt cggtgacgag
    17941 gccgtgattg gcggtggcaa gctcaagcgg cacgagtttc tgccacagcg cgaagagatc
    18001 ggagctgcgc atatgcggca gcaccagatt ctgctcgtgc gtcacgcgga tttcggcctg
    18061 cccgaacgta ttcatgagag cggcgatggc gtccatctgc tccgatgaac aatcgcccgg
    18121 agtcttgccg atgggtttga gagagagtgt cgcgatggcg tagcccggca tcttgtgggg
    18181 cgcgacgttg gtcttcaccc aatcggcgaa atcggaatgg ctccgtttgg ctgcttcgaa
    18241 cgcctcgcat cgtgccggta gcgtctcaaa cggcgggtcc gcgaaataag cgccgatgcg
    18301 cgcgagttcc tcggcgggaa gctcaagcac ccctgtcttc ttgatctcga tgaattcggc
    18361 ttctacgcgg cggcgcatct cctcgatgcc gatctgatgg accaggatct tgacgcgcgc
    18421 cttatggatg ttgtcgcggc gcccggcctc gttgtagacg cgcaggatcg cctcgcagaa
    18481 ggccaggatg tcgggcttcg caacgaagga accgatttcc agcgcgagat aaggcgtgcg
    18541 cccgaggccg ccgccgacat agacgcggta gcccagttcg cccgaatcgt tgcggatgac
    18601 cttgatcgcg atgtcgtgga tcaaaagcgc tgcccggtcc tcgtccgagg cgatgacgca
    18661 gaacttgaac ttgcgcggca ggaacgagaa ttcggggtgc agcgtcgacc attggcgcag
    18721 gatttcggac aggatgcggg gatcttccag ctcaccggcg gccgcgcccg cgaatggatc
    18781 ggcgctgatg ttgcgcacgc tgttgccgct ggtctggatg cagtgcatct cgacttcggc
    18841 gagttccacc agcatgtcgg ggacgcgcgg cagttgcgcc caatggagct ggaggttctg
    18901 ccgcgtggtg aaatggccgt agccgcgatc atatttgcgc gtgagatgag caagtttgcg
    18961 caactggcgc gcgctcaggg tcccataggg aatggcgatg cgcagcatgt aagcgtgaag
    19021 ctgaagatac agcccgttca tcagccgcaa cggcttgaac tcgtcctcgg tcagctcgcc
    19081 ggaaatccgc cgcgccacct ggccgcggaa ctgctcggcg cgctcgcgca cgaattgcgc
    19141 gtcgaattcg tcatagcggt acatcagcca gtcttctttc caaggccagt cttctctcca
    19201 aggtccgtct tctttccaag gtccgcctgc tttcccagat ccgtgcggac ggtaggtcct
    19261 gccgcgcgga ccgcctcgcg caccttcacc ggcaccgtgc gccccgccgt ttcgtgggcc
    19321 tcgaagagat agggcgccac gatttcgcca ctgcgcacga attcgcccgc acccttgagc
    19381 gccgcctcgg caacggcccc ggacatcaat tcggcctcat caagccggga aacccagcgg
    19441 ccctggttcc agtaagcaac ttcgccgagc ttcagccgat tggcggttag gatttgcatg
    19501 agaaaagcgc accttgcaaa gcgggggcta aaatcacatt ccggatcgga gaaatcaaga
    19561 gatcacataa ttttcgtgta aatatttcta attaacatga tttatttgtt atatatcttt
    19621 cttgagcgcg ctctcatgcc atttgcggag cactagatat tccacgagcg ccagcgcgaa
    19681 aaagatcata acccccagag ccgcaagaag aaacaacgcc gcgaacatgg tggcgatctg
    19741 caaccggttg cccgcttcca cgatgcgcca ggcaagcccc gtggcggtgc cggagcccgc
    19801 gccatattcc gccaccacgg tgccgatgag agcaagtccg ccggatgtct tcatggcgtt
    19861 gagcagaaag ggcagggcgg tgggcaggcg cagccgggtg agaatttgcc agttggaggc
    19921 gccgtgcagc cggaacagat caaccagatt gtgatcggcg gagcgcaaac ccagcgtggt
    19981 gcccgagagg atcggaaaga aggcgacaat ccaggcaagg atcagaagcg ccagatcgat
    20041 atgcgcaaat cccacccaga tgacgatcaa gggcgcgatg gcgaccaccg gcgtcacttg
    20101 caggatcacc gcatagggat aaagcgccat ttcgacgatg cggctttgcg aaaagaacac
    20161 ggccagggcg accccgctga cgaccgcaag cagaaaggcc agcagcgtga tccgtaaggt
    20221 cgtccagagg gaggccatca gcgagggaaa atcagcgatc aatgccgccc agatcgccga
    20281 tggcgccggc agcacgaaac gcggaatgtc ataagccgcg acgagccctt cccagagggc
    20341 cagcaccgcc acgcccagcg cgagcggaag caacaggcgg agcgtgcggg acacattcat
    20401 catgccgccg ccttggccgc ggcatcgcga agcccgcgcg agacagcccg cgcggcctcg
    20461 gcgaattcgg gcgccatccg ccattgcgat gtgcggtccg gcggcgaggc aagccggaat
    20521 tcctgtgcga cgcggccggg gcgcggcgtc atggtcacga ttcgggtcga caggaacgtg
    20581 ctctcataaa cgctgtgggt gacgaagacg atggtgagtc catcttcgcg ccagagcttc
    20641 agcagatcct cgttcaacgc atcgcggctg atctcatcga gcgccgcgaa gggctcgtcc
    20701 atcaacagaa gctgcggttt ggcggccaac gcgcgcgcga tggaaacgcg catccgcatg
    20761 cccccggaaa gcacgcgcgg gtaggcaccg gagaatcccg tgaggccgac acgcgccagg
    20821 gcgcgttcag cctctgcatc ggcgtctgcc cgcgctacgc ccgccaggtc gagcggcaga
    20881 cgcacgttgt cgcgcacgat agcccagggc atgagtgtcg gatcctggaa tacgaacccg
    20941 atgcggggct tagtacgcgg ccaatccagc gtgccggcgc tcggcgccag aagcccggcg
    21001 atgatgcgca agctggtgga tttgccgcat cccgacgggc ccagcaggga caggaactcc
    21061 cccctctcga tcgcgagacc gagtggacca agtgccttca cgccattgct gaagacgcgc
    21121 tcgacggcgc gcaacgacgc aacgatggtc atattcctgc cactccgccg cgcgggtatc
    21181 tcctgcgggg accgtaacag cgtgggatga aatttatcac cgtgattttc gcggcacctc
    21241 ccaaccagca cagctattag gcccttaacg cgggaccgcg cccttggcgc ggaacgcata
    21301 agcgatgaag tggaggcagg cggtggcatt gtggcgtttg atatttcctc tcctatgttc
    21361 tgcgttcata cccgagatgg catgggccga tcccatcccg gagcccgttg cgcggattat
    21421 cctgaaggcc gccgggagcg gggatcccgc gaccctgcaa tcggttgtgg atatcgccaa
    21481 ggcgaccaat ccggcttcgg ttgcggaaat cgacgcgctc gcggcccgat tgcgcaagga
    21541 ggcggaggcc acgcgaatcg caaagctgag ttcggaaaca ttcttcgagg gctggacggg
    21601 cgaaggggaa gccggcgcgt ccctgacgac aggcacgtcc gataacaaga cgctcgcgct
    21661 cggcgtgcat ctcaacaagg aagacctcga atggcgtcac aagatcgcgg ccgtcgccaa
    21721 ttatcaaagc agcgacgatg tcacgacctc ggaaagctat ctcgcgagct atgaaggaaa
    21781 ttaccggttt gcgccgcgcc tttacgcttt tggtttgctg caatgggaac aggacaggtt
    21841 cgccggtttc aacgcccgtt attcggaatc gttcggcgcc ggctacacga tcgtcgatac
    21901 gcccgcgttc aactggcaga tatcgaccgg cccggcgctc cgccagaccc ggcgggtcac
    21961 ccatgaagcg gaaagcgacg cctccatccg agccgacacc ctgctgctct ggaatctcag
    22021 tccaagcacc gcgttcaacg aagagctcgg cacctatgcc gggggaagcg acagcaccta
    22081 tttttccacg acgtcgctga cgaccaagat catgggaaac ctttcggcgc gcacgtcttt
    22141 caacatcacc tcggaaagca atccgcccca aggcatcgac aacaccaaca cgatcacacg
    22201 cttgaccctg gtctatagtt tttgaaatgc cgcgagggcc gatgggggct gcggcgttac
    22261 tttgctgccg cgtgccgatc gttcagcgcg tagcccttgt cgatgaattg cagcgtatag
    22321 gcgtcgcgat aatccaggtt cgccggatac acgttctgtt tcgacatgac gtcgaagaac
    22381 tcctgccagc gggcatcggt catcgcgcca agaccgagcc gctcggcgtc gccggacagc
    22441 gcgatgccat aggtcttcat cttgtcgatg gcctgggcga gcacatcgtc ggtcatatcg
    22501 ggattgtcgc gcttgatgag cgcattgcct gccgagggat cgccatagag ataatgcatc
    22561 cagccttcga tgctggcatc cacgaaggcc tgcacggcat caggatgttc ggcgatccat
    22621 ttctcgggca ccagaaccat gtttgcatag ccgggatagc cggcgtcggc gagcgggaag
    22681 acctggggcg tgaacttgcc ttccttttcg accgtatagg gttcggacgt cagataacct
    22741 tcctgtatcg cattcttgtc caccaggaat ggcgccagat tgaaggtgta tttgcggatt
    22801 tggctatcgg cgaacccgaa tttcgtcttc agccagggcc agaacgtcac cgtcgacgcg
    22861 tcgccgatca tgatgggttt tcctttcatg ccggcaatgg atttgatgtc gtcgcgcggg
    22921 tgcgtgatga gcacttgggg gtccttctgg aagaccgcca tgaccgcttt gatcttggcc
    22981 ccttcgcgga cgaggttcag cgcgatgaaa ttgtttgagc ccatcccgaa atcggccgcg
    23041 cctccggcta gcaattgcgg cacgttcacc gcggctccgc cctggagaag ggtgacgtcc
    23101 aggccgcggc gggcatagag cccttcggcg agcgcctgat agaatccgcc atgctcggcc
    23161 tgcgccttcc aatcggtgac gaagccgata tgcacctgcg gctttgccgg ggttggcgtt
    23221 ttcggcgttt cggcggggcc gcacgccgtc gccgcgagca atgccgcggc ggcaaggcat
    23281 cgtccgagcc cacgcatatg acgctccttc ttggatgacc catgaggctt aaatgaccca
    23341 tgagggtgca ggcatgggcg cggagaagca agctcaaggg atgagtggcg gggtttgacg
    23401 cgcaggccgg ccctgaccgg tctggaaaac ctgacgttct ccggtttcgc cgttttcaaa
    23461 cacaaaggtc tcgcccggcg gtgcgggcgg tgttgcatgg gtcctggcct ccgatgtctc
    23521 cggaggaggg gagatgtctt tcgacgccgg ccccgatgcg atgtttgcat gtccgccgcc
    23581 gaacagatga tcgaagaaat tcccgattcc gtcgaggccg cgctggatcg gattatcgcc
    23641 cccctgcggt gtgttgccgt cttccgcgat gaaaggcggc acaggttcgg cgcgcggcag
    23701 atgcgtgtgc ggactgttct tcagtgcggt gcgcatgaag ccgctccaga tctgcgccgg
    23761 cagcgagccg ccggtgattt ttttcatggg tgagaaatcg tcattgccga cccagacgcc
//
LOCUS       DASYEG010000091         8812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4378087, whole genome shotgun sequence.
ACCESSION   DASYEG010000091 DASYEG010000000
VERSION     DASYEG010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 8812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8812
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4378087"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="VGM72_03485"
     CDS             <1..566
                     /locus_tag="VGM72_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264354.1"
                     /translation="IGMSKKVPRLEMEQLAPNVQETLAARVKRLGYLGEFFKCSGHNP
                     DVLVPFMEMTEALKEALPDRLTEVGSLTVAGLMGNDYERNQHERLSEKLGFGRDWVAA
                     VNRLQPDALGPMSEDERAVQRLALALIARHGKNVDAEFDALIERIGHRQAMAVLFLIG
                     RYVMHAYVVNALNLAPPVPSIFAGDKA"
     gene            563..1549
                     /locus_tag="VGM72_03490"
     CDS             563..1549
                     /locus_tag="VGM72_03490"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEY4264355.1"
                     /translation="MTDPLWITEADVVAALSLPEAIPALEAGLRLEAAGTARNMEKTH
                     QIWGGHHTLHAIGAVVEGAHIVGTKTWAHTAGGATPLVTLWNSDTGKLEAIIEAFALG
                     QMRTGGISGVATARMAREDADELAIVGTGKQALPQVMAVAAVRPLKRIRVFSPTPENR
                     CAFMARLETLLPAARLIEATSVEAAVKDAPIVTLVTRAREPVLTAAMIAPGTHLNAVG
                     AITPEREEFTQDVFGRVGAIAVDMVESVKSLSAEFVTRFGAGDWSAVRPLSELVAVNA
                     TRPKNADITLFKAMGMGLSDLALGIEILKRVRASGGGRPIPSPKPAQPRLVS"
     gene            1572..2426
                     /locus_tag="VGM72_03495"
     CDS             1572..2426
                     /locus_tag="VGM72_03495"
                     /inference="COORDINATES: protein motif:HMM:NF027552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4886 domain-containing protein"
                     /protein_id="HEY4264356.1"
                     /translation="MKRLVALSVLAVIALAEPASAKTILFVGNSFTYGELSPVRHYKA
                     NTVTDLNKTAIGGVPALFKAFATEAGLDYDVSLETVPGVGLDYHYAERRSLFDRKWDA
                     VVLQSYSTLDQNHAGDPGMLTKYAGLFSDMFHKRNADVKIYLTATWSRADMTYPNGMP
                     WHGKPITQMGKDVDAGYRIAAQKAPGIAGVVPVGLAWNEAIDKGIADPNPYDGIAANQ
                     INLWAVDNYHASTYGYYLEALLVFGRVTGIDPLKVGDHEKAIGELSLDPAVIRKLEQI
                     AHDELAAH"
     gene            2444..3529
                     /locus_tag="VGM72_03500"
     CDS             2444..3529
                     /locus_tag="VGM72_03500"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEY4264357.1"
                     /translation="MRNDYFRDCTGATPRPLDLWEPIVIPKEEIDAEIERLANLPTPA
                     NGRRRSVFVHPRNQKSQGLAPGIEVALDVLKPGERTVPYRQNSTQVNFVIQGRGTSVV
                     GGRRWDVNLYDVWNTPSMQPYWHQNTGDGLFVRLTYSNAALMEMMNVHYVEENPPPVQ
                     AVAHEALSEEDKKRRASPFGTFKLNEEGAWLMPYEILINPPTVESPALHWPWAEVKSH
                     LDKLEALGKDYVGRRLYLLYNPMTGRTNGTTPNFFATMTIRPPKIVDRPHRHTSAAIN
                     YYFHGTGRSTVEGKVYQWKAGDLMLSAPGWAVHNHASHDDYVYELTVQDQPLNIAMES
                     LLWQESLKLPMAVLGVEEGVGTNRAAG"
     gene            3532..4434
                     /gene="dapA"
                     /locus_tag="VGM72_03505"
     CDS             3532..4434
                     /gene="dapA"
                     /locus_tag="VGM72_03505"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEY4264358.1"
                     /translation="MIRLDAHQLKGSFPPVVTPFKDDGAVDYDTYAKLVEFHVTGGSP
                     GIVVTGTTGEPSTLTSDERTQLLKVAIGAANKRIQVVAATGTQNHADTVVMTEAAAKA
                     GADAVLIVTPYFIRPPQRGLKAYYLDLCKRSDLPVMIYHIPGRAVVNVTAETVVRIAE
                     AAPNLVGIKHAVNDLALVSDLIAKLGAEFRIHVGLEDLSFPMVCLGAHGLMNAVGNLL
                     PGKVQELYAATAAGDLTRGRALHYELWELNQAVFFDTNPIPMKYMMKRMGLLPNEAHR
                     LPMVPTTPELRARLDAVLARAGLV"
     gene            4437..5174
                     /locus_tag="VGM72_03510"
     CDS             4437..5174
                     /locus_tag="VGM72_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY4264359.1"
                     /translation="MAKNALAEIDTALIQDLVDANRILYHLGVVDGFGHVSARDPNDS
                     SRFIISRSMAPALVTADDLMAIHLDGRPVSQGAPTPYLERFIHGEIYAARPEVVAVVH
                     SHSAAIIPFTIVPEATLKPVSHMGGFIGEGAPVFEIRDTAGPDNDMLVRSRELGQALA
                     KSLGGHNIVLMRGHGSTVVGFNLRQAVYRAVYAELNARQQSEALRLGTPIYLTPGEAV
                     ESAKANDGQQNRAWDLWKRAIDARGLA"
     gene            complement(5178..6284)
                     /locus_tag="VGM72_03515"
     CDS             complement(5178..6284)
                     /locus_tag="VGM72_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4264360.1"
                     /translation="MLQVENLRKQYVSFEGKPGGGVLGVGFDIAEGELFTLLGPSGCG
                     KTTTLRAIAGLETPDSGIIQIAGREMFNAERGVNVALYDRDIGMVFQSYAIWPHMSVF
                     ENAAYPLRVSRTHRSSGRDIEKKVMAVLDMVGLGAFAARPATQLSGGQQQRLALARAF
                     TREPRLLLLDEPLSNLDAQLREQMRVELKRLQQETGVTAVYVTHDQAEALAISDRIAV
                     MHEGKIAQIGRPKEIYSRPSCEFVANFIGRTNLIKGVPSRNVAAESVGPVTTDLGELL
                     CFFPNACAAGRAISLVVRPEHIAIANGDPGSRETNRLSGHVRRETYLGEIVEYLVSVG
                     GGELLMRTQFGGAAKDDAITLQFSAGQTVALAQS"
     gene            complement(6303..7379)
                     /locus_tag="VGM72_03520"
     CDS             complement(6303..7379)
                     /locus_tag="VGM72_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY4264361.1"
                     /translation="MSTRRAFTFGWVMAALIATMPALAAPAPNIATYKGADREQLILA
                     GAKREGKVVFYSGMIENQALRPMADAFRRKYPFVTVEYWRGDSRGLVQKALTERRAGR
                     VIGDILESTGGAEALIKAGAVEAFASPSTAGFPKNYLDPNGMWAASRLDYFGMAYNTR
                     QLRAADVPKTYDDLLNPKWKGAIAWRADSEVGADLFIAGVMREMGKEKGEAYLKKLAG
                     QDIVNYAGSARALVDRVGEGEYKIALEIYAHHPLISKAKGAPLDTQMLDPVPSALSTI
                     QLTKNAPHPNAAMLFIDFALSREGQEVLRAAQYLSPNPEVDTDPALRKIIPRLNGLKE
                     TVFTPELMFQSRDEANALFNKYFR"
     gene            complement(7376..8473)
                     /locus_tag="VGM72_03525"
     CDS             complement(7376..8473)
                     /locus_tag="VGM72_03525"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY4264362.1"
                     /translation="MIVARVLLEPCVMRKDDPKWRFALGASPVTEGVVVTLVAENGVC
                     GFGYGSATPHMGATRESLMAALTRFVPLVTGRDCFAVEEILQVLDADSAGHHQAKAAI
                     DCALHDLNARLLNIPLHQMFGGKLRDSVPILRILAIKTPDEMATQAQKLADAGYRYLK
                     VKVHGDVKEDVARVRAIRRQVGDAVRLTIDANQSYSVKDAISALTRMDECDIDLAEQP
                     VAAGDLAGLKLVTESVPVTVEADESAASLADVMHLVSNRIVDAVSLKIPKLGGLRNTL
                     AAARICEAGHVRHRMGAAVGSRLLSAHAMHLAATLPGLDYACELGEFDRLKDDPFEGI
                     EIENGHLRLPQGPGSGVRPRDVPRTLKAVES"
     gene            complement(8479..>8812)
                     /locus_tag="VGM72_03530"
     CDS             complement(8479..>8812)
                     /locus_tag="VGM72_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264363.1"
                     /translation="LRWGFIEVSAEWLPYAFNDLELRFKRKDRKFPTDPLKTNNMYVA
                     CQCTDDLGYILKHGGADNLVIGTDYGHADTSAQIEALRIVRKEGKVDASVIDRILDDN
                     ARALYGLD"
BASE COUNT         1647 a         2739 c         2763 g         1663 t
ORIGIN      
        1 caattggcat gagcaagaaa gttccccgcc tggaaatgga gcaacttgcg ccaaacgtcc
       61 aggaaacgct cgcggcccgc gtgaagcgtc ttggctatct cggcgagttc ttcaaatgct
      121 ccggccacaa tcccgacgtg ctggtcccct tcatggagat gacggaggcg ttgaaggaag
      181 ccttgcccga ccggctgacg gaagtcggct cgctgacagt tgcgggcctc atgggcaatg
      241 attatgagcg caatcagcat gagcggctgt ccgagaagct tggcttcgga cgcgattggg
      301 tggcggcggt caaccggctt cagcctgatg ctctaggccc gatgagcgag gacgagcgcg
      361 ccgtgcaacg actggcgctg gcgctgatcg cgcgccacgg caaaaacgtc gatgcggagt
      421 tcgacgcgtt gatcgagcgc atcggccacc gtcaggccat ggcggtgttg tttctcatcg
      481 gacgctatgt catgcacgcc tatgtggtga atgcgctcaa tctcgcgccg cctgtgccct
      541 cgatttttgc cggagacaaa gcgtgaccga tcccctgtgg atcaccgaag ccgacgtcgt
      601 ggccgcgctc agcctgcccg aagccattcc cgcacttgag gcggggctgc ggctggaagc
      661 ggccggcacc gctcgcaaca tggaaaagac ccatcagatc tggggcggcc atcacacgct
      721 gcacgccatc ggcgcggtgg tggaaggcgc tcatatcgtc ggcaccaaga cctgggcgca
      781 caccgccggc ggcgcgacgc ctctggtcac gctgtggaac agcgataccg gaaaactcga
      841 agccatcatc gaggcgttcg cgttggggca gatgcgcacc ggcggcatct cgggcgtggc
      901 cacggcgcgc atggcgcgcg aggatgcgga cgagctcgcc atcgtcggca ccggcaagca
      961 ggcgcttccc caggtgatgg cggtcgccgc cgtcaggccg ttgaagcgca ttcgggtctt
     1021 cagcccgacg ccggaaaacc gttgtgcctt catggcgcga ttggagacat tgctcccggc
     1081 ggcaaggctg atcgaggcca catcggtcga agccgcagtg aaggatgcac cgatcgtcac
     1141 gctggtgacg cgcgcgcgcg aacccgttct caccgcggcg atgatcgcgc caggaaccca
     1201 cctgaatgcg gtcggggcga tcacgccgga gcgcgaggaa ttcacccagg atgtgttcgg
     1261 ccgggttggg gcaatcgctg tggacatggt ggagtcggtc aaatcgctga gcgctgaatt
     1321 cgtcacgcgc ttcggcgccg gcgattggtc ggcggtacgg cctttgtcgg aattggtggc
     1381 ggtgaacgcg acgcgtccga agaacgccga catcacgttg ttcaaggcga tggggatggg
     1441 cttatccgat ctcgcgctcg gcatcgagat tttgaaacgc gtgcgtgcaa gcggcggcgg
     1501 gcggccgatc ccgagcccca agcccgccca accgcgtttg gtttcataat cacccgcagg
     1561 agggacatag cgtgaaacgt ctcgtcgccc tgtctgttct cgcagtgatc gcgctggctg
     1621 agcccgcttc cgccaagacg atcctcttcg tcggtaacag tttcacctat ggcgagctgt
     1681 cgcccgtgcg gcactacaag gctaacaccg tcaccgatct caacaagacc gctatcggcg
     1741 gggtgcctgc gctgttcaag gccttcgcga cagaggccgg gctcgattac gacgtcagcc
     1801 tcgaaaccgt gcctggcgtc gggctcgatt atcattatgc cgagaggcgt tcgctgttcg
     1861 accgcaaatg ggatgcggtc gttttgcaga gctacagtac gctcgaccag aatcatgccg
     1921 gcgatcccgg catgctgacc aaatacgccg gactgttttc cgacatgttc cataagcgca
     1981 atgcggacgt gaaaatctac ctcaccgcga cctggagccg tgctgacatg acctatccca
     2041 atggcatgcc ctggcacgga aaacccatca cccagatggg taaggatgtc gatgccggtt
     2101 acagaatcgc ggcccaaaaa gcgccgggga tcgcgggcgt tgtccccgta ggccttgcct
     2161 ggaacgaggc tatcgacaag ggaattgccg atcccaatcc gtatgacggc atcgcggcaa
     2221 accagatcaa tctctgggct gtcgacaatt atcacgccag cacttacggc tattatctgg
     2281 aggcgctgtt ggtgttcgga cgggtcacgg gcatcgatcc gctgaaggtc ggtgaccacg
     2341 aaaaagccat cggcgaactg tcactggacc cagcggtgat ccgcaaactc gaacagatcg
     2401 cccatgacga attggccgcg cattgaggat cacggagttg accatgcgta acgattattt
     2461 ccgcgactgc acgggcgcca cgcccaggcc gctggacctt tgggagccga tcgtcattcc
     2521 caaggaggag atcgacgcgg agattgaacg tctggccaat ctgccaacgc ccgccaatgg
     2581 caggcgccgc tccgtctttg tccatccgcg caatcagaaa tcgcaaggtc tggcgcccgg
     2641 catcgaagtg gcgctcgatg tgctgaagcc cggcgagcgc accgtgccct accgccagaa
     2701 ttcgacgcag gtgaatttcg tcatccaagg ccgtggcacg tccgtggtgg gcgggcggcg
     2761 ctgggacgtg aatctctatg acgtctggaa cacgccttcg atgcagccct attggcacca
     2821 gaacaccggc gacggattgt tcgtgcgact tacctattcc aatgccgcgc tgatggagat
     2881 gatgaacgtc cattatgtcg aggagaatcc accgcccgtg caggccgtcg cgcatgaggc
     2941 gctgtcggag gaagacaaga aacgccgcgc cagccccttc ggcacgttca agctgaacga
     3001 ggagggcgcc tggctgatgc cttatgaaat cctgatcaat ccgccgacgg tggaatcgcc
     3061 cgcgctgcat tggccatggg ccgaggtgaa gtcgcatctc gacaagctcg aagcgctcgg
     3121 caaggattat gtcgggcggc ggctctatct gctctacaat ccgatgacgg gccgcaccaa
     3181 tggcacgacg ccgaatttct tcgcgaccat gacgattcgc ccgccaaaaa tcgtggaccg
     3241 cccgcaccgg cacacgtcgg cggcgatcaa ttattatttt cacggcacgg gccgctcaac
     3301 cgtcgaaggc aaggtctatc aatggaaggc gggcgatctg atgctctcgg cgcccggctg
     3361 ggcggttcac aatcacgcct cgcatgacga ttacgtctat gagctgacgg tgcaggatca
     3421 gccgctcaac atcgccatgg aaagtctgct gtggcaggag agcctgaagc tgcccatggc
     3481 ggtcttgggc gtcgaagaag gtgtcggcac caaccgggcg gcgggctgac catgatccgg
     3541 ctcgatgcgc atcaactcaa aggttcgttt ccccccgttg tgacgccatt caaagacgac
     3601 ggcgcggtcg attacgacac ctacgcgaaa ttggtggagt ttcatgtcac gggcggttcg
     3661 ccgggcatcg tggtgacggg cacgacaggg gagccatcga cgctgacgtc cgatgagcgc
     3721 acgcaattgt tgaaagtcgc aatcggtgcc gccaacaagc gcatccaggt ggtggcggcg
     3781 acgggcacgc agaatcacgc cgacacggtg gtgatgacgg aggccgctgc gaaggcgggc
     3841 gcggatgcgg ttctcatcgt cacgccctat ttcattcgcc ccccgcagcg tgggctcaaa
     3901 gcctattatc tcgatctgtg caagcgctcc gatctgccgg tgatgatcta tcacataccc
     3961 gggcgcgcgg tcgtgaacgt caccgccgaa acggtggtgc ggattgccga agccgcgccc
     4021 aatcttgtcg gcatcaagca cgcggtcaac gatctcgctc tggtcagcga tctgatcgcg
     4081 aaactgggag cggaattccg catccatgtc gggcttgagg atttgtcctt tccgatggtg
     4141 tgtctcggcg cgcatgggct gatgaatgcg gtcggcaatc tcttgcccgg caaggtgcag
     4201 gaactctacg cggcgacggc cgccggcgat ctgacacgcg gacgcgcgct gcattatgaa
     4261 ttgtgggagc tgaatcaggc cgtgttcttc gataccaatc cgatcccgat gaaatatatg
     4321 atgaagcgta tgggcctctt gccgaacgag gcgcatcgtc tacccatggt gcccaccacg
     4381 ccagaattgc gcgccagact cgacgccgtc ctggcgcgcg cgggtctcgt ctgaccatgg
     4441 cgaaaaacgc gctcgccgaa atcgatacgg ccctgatcca ggatctggtc gatgccaacc
     4501 ggattctcta tcacctcggc gtggtcgatg ggttcggcca tgtcagcgcg cgggacccga
     4561 acgattcctc gcgcttcatc atttcgcgca gcatggcgcc ggccctggtt acggccgatg
     4621 atctgatggc gatccatctc gacggccgtc ccgtttcgca aggcgcgccc acgccctatc
     4681 tcgaacgctt catccatggc gagatttatg ccgcccggcc cgaagtggtc gcggttgtgc
     4741 atagccattc ggctgcgatc atcccgttca ccatcgtgcc ggaggcgacg ctcaaacccg
     4801 tgtcgcatat gggcggcttc atcggagagg gcgcgccggt gttcgaaatc cgcgataccg
     4861 ccggaccgga caacgacatg ctggtgcgct cgcgcgaact cggccaggcg ttggcgaaat
     4921 cgctgggcgg ccataatatc gtgctgatgc gcggccatgg ctcgacggtg gtggggttca
     4981 atctgcgcca ggcggtctat cgcgcggtct atgccgagct gaatgcgcgt cagcaatccg
     5041 aagccctgcg gctgggcacg ccgatctatc tgacgccggg cgaagcggtg gaatcggcca
     5101 aggcgaatga cggtcagcag aaccgcgcct gggatttgtg gaagcgcgcg attgacgcgc
     5161 gcggcttggc gtaaacctca gctctgcgcc agcgcgactg tctgtccggc ggagaattgc
     5221 agcgtgatcg catcgtcttt tgccgcgccg ccaaattgcg tgcgcatcag gagttcgcca
     5281 ccgcccacag acacgagata ttccacgatc tcgcccaaat aggtttcgcg ccgcacatgc
     5341 ccgctcagcc ggttggtttc gcgggagccg ggatcaccat tggctatcgc gatgtgttcg
     5401 ggccgcacca ccagggaaat tgcgcgtccc gccgcgcagg cgttcggaaa aaagcacagc
     5461 agctcgccca gatcggtcgt caccggtccc accgattccg ccgcgacatt ccgtgacggc
     5521 acgcctttga tgaggttggt gcggccgatg aaattggcga cgaattcgca cgacgggcgg
     5581 ctatagattt cctttggccg gcctatttgc gcgatttttc cctcatgcat gacagcgatg
     5641 cggtcggaaa tggccagcgc ctcggcctgg tcatgcgtga catagacggc ggtgacgccg
     5701 gtctcctgct gcagccgctt caattcgacg cgcatctgct cgcgcaattg cgcatccaga
     5761 ttggacagcg gttcatcgag caacaacagg cgcggttcgc gggtgaaggc gcgcgcgagt
     5821 gccagacgct gttgctggcc gccggaaagc tgtgtggcgg gccgcgccgc gaacgcgccg
     5881 agcccgacca tgtcgagcac ggccatcact ttcttctcga tgtcgcgccc agaactgcga
     5941 tgcgtccgcg atacccgcaa agggtaggcg gcgttttcga acacgctcat atgcggccag
     6001 atggcgtagg actggaacac catgccgatg tcgcgatcgt aaagcgcgac attgacgccg
     6061 cgttccgcgt tgaacatctc gcgtccggcg atttggatga ttccgctgtc cggcgtttcc
     6121 agccccgcaa tcgcccgtag cgtcgtcgtc ttgccgcagc ccgatgggcc cagcagcgtg
     6181 aacaattcgc cttccgcgat gtcaaagccg acgccgagca cgccgccgcc gggcttgccc
     6241 tcgaagctca catattgctt gcggagattc tcgacctgaa gcaactcgcg cccctcttcc
     6301 actcagcgga aatatttgtt gaacagcgcg ttggcttcgt cgcgggattg gaacatcagt
     6361 tcgggcgtga atacggtttc cttcagcccg ttcaagcgcg ggatgatctt gcgcaaggcg
     6421 ggatcggtgt cgacttccgg gttcggcgag agatattgcg ccgcgcgcaa cacctcctgg
     6481 ccctccctgg acagcgcgaa atcgatgaac agcatggccg cgttgggatg cggcgcattc
     6541 ttcgtgagct ggatggtgct gagcgcggat ggcacgggat cgagcatctg cgtgtccagg
     6601 ggcgcgccct tggctttgct gatcaaagga tggtgcgcat agatctccag cgcgattttg
     6661 tattcgccct cgccgacacg atccaccaga gcgcgcgcgc tgcctgcgta attcacgatg
     6721 tcttggccgg cgagtttttt aagataagcc tcgcccttct ccttgcccat ttcgcgcatc
     6781 acgcccgcga tgaagaggtc cgcacccact tcgctgtccg cgcgccaggc gatagcgcct
     6841 ttccatttgg ggttcagcaa atcgtcatag gttttcggca cgtcggccgc gcgcaattgg
     6901 cgcgtgttgt aggccatgcc gaaataatcc agacgcgaag ccgcccacat tccgttcgga
     6961 tcgagataat tcttcgggaa gccggcggtg gagggcgatg caaaagcctc gactgcgccg
     7021 gccttgatga gcgcctccgc accgccggtg gattcgagga tatcgccgat gacgcgtccc
     7081 gcgcgccgct cggtcaaggc cttctgcacc agcccgcggc tgtcgccacg ccaatattcc
     7141 accgtcacga agggatattt tcgtctgaag gcatcggcca tcggacgcag cgcctgattt
     7201 tcgatcatgc cagaatagaa gacgactttg ccctcgcgct tggcgccggc caggatgagc
     7261 tgctcgcggt cggcaccctt ataggtcgcg atattgggcg cgggcgccgc caacgccggc
     7321 atggtggcga tcagcgcggc catgacccag ccgaatgtga aagcgcggcg cgtgctcacg
     7381 attccaccgc cttcagcgtc cggggcacgt cacgcgggcg gactccgctg ccgggaccct
     7441 gtggcaggcg aagatggccg ttttcgattt cgatgccttc aaagggatcg tccttcagcc
     7501 ggtcgaattc tccgagttca caggcgtaat cgaggcccgg cagcgtggcg gccagatgca
     7561 tggcgtgcgc ggacaacagc cgcgagccga ccgcggcacc catgcggtgg cgcacatggc
     7621 cggcctcgca gatccgcgcc gcggcaaggg tgttgcgcaa gccgcccagc ttggggattt
     7681 tcaggctcac cgcatcgacg atccggttgg agaccagatg catgacatcg gcgaggctgg
     7741 cggcgctctc gtcggcttcc acggtgaccg gcacggactc cgtcaccagc ttgagaccgg
     7801 cgagatcgcc cgcggcgacc ggctgctccg cgaggtcgat atcgcattcg tccatgcggg
     7861 taagcgcgga aatcgcgtcc ttcaccgaat aggattggtt cgcgtcgatg gtcaggcgca
     7921 ccgcgtcgcc cacctgacgc cggatggcgc ggacccgcgc cacatcttcc ttcacatcgc
     7981 catgcacctt gaccttcaga tagcgatagc cggcatcggc gagcttctgc gcctgcgtcg
     8041 ccatttcatc gggcgtcttg atcgcgagga tgcgcaggat cggcaccgag tcgcgcaatt
     8101 tgccgccgaa catctggtgc agcggaatat tcaggagccg cgcgttcaga tcgtgcagcg
     8161 cgcaatcgat cgccgccttg gcctgatgat ggccggcaga atcggcatcg agcacctgca
     8221 atatctcttc gaccgcgaag caatcgcgcc ctgtcaccag cggcacgaaa cgggtgagcg
     8281 cggccatcag gctttcgcgc gtcgccccca tatggggcgt ggccgaacca tagccgaagc
     8341 cgcaaacgcc gttctcggcg acgagtgtca ccaccacgcc ttccgttacc gggctggcgc
     8401 cgagcgcgaa gcgccatttc ggatcgtctt tgcgcatcac gcaaggctcg agtagaacgc
     8461 gcgcgacgat catcggcttc agtccagtcc gtagagcgcg cgcgcattgt cgtcgaggat
     8521 gcggtcgatc accgaggcat cgaccttgcc ttccttgcgc acgatgcgca gcgcctcgat
     8581 ctgggccgag gtgtcggcgt ggccgtaatc cgtgccgatg accagattgt cggcgccccc
     8641 atgtttgagg atgtagccga gatcatccgt gcactgacag gcgacataca tattgttcgt
     8701 cttcagcgga tcggtgggga atttccggtc cttgcgcttg aagcgcagtt ccagatcgtt
     8761 gaacgcatag ggcagccatt cggcgctcac ttcgatgaaa ccccagcgca gc
//
LOCUS       DASYEG010000092         2758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2783296, whole genome shotgun sequence.
ACCESSION   DASYEG010000092 DASYEG010000000
VERSION     DASYEG010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2783296"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VGM72_03535"
     CDS             <1..292
                     /locus_tag="VGM72_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007702574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY4264364.1"
                     /translation="AQAEAFYAVHSARPFFNGLVAFMTSGPVVVQVLEGEGAVLKNRE
                     VMGATDPAKAAPGTIRKDFAENIEANSVHGSDAPDTAANEIRFFFSDLEITG"
     gene            complement(282..869)
                     /gene="purN"
                     /locus_tag="VGM72_03540"
     CDS             complement(282..869)
                     /gene="purN"
                     /locus_tag="VGM72_03540"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEY4264365.1"
                     /translation="MSAGRLRIAVLISGRGSNLKALIDACAARDYPAEIVAVISNVAD
                     AGGLKFASDAHIPTVVISHKAFASREAFDTAMDKRLRDAGVELICLAGFMRILSDGFI
                     RAWQGRLINIHPSLLPAFKGTNVHERVLATGEKMSGCTVHYVIPELDSGPAILQSSVP
                     VQLEDTPETLAARVLEAEHMLYPQAVKIVAGSISR"
     gene            complement(866..1936)
                     /gene="purM"
                     /locus_tag="VGM72_03545"
     CDS             complement(866..1936)
                     /gene="purM"
                     /locus_tag="VGM72_03545"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEY4264366.1"
                     /translation="MSTGKPSVTYKDSGVDIDAGDALVDRIAPAAKATRRAGADAALG
                     GFGGLFDLKAAGFKDPLLVASTDGVGTKLKLAIEAKKFDTIGQDLVAMVVNDIVVQGA
                     EPLFLLDYYASSRLDVDEAAIVIEGIARACKESGCALIGGETAEMPGLYAKGDFDLAG
                     FAVGAVERGQVLPDLAAIRAGDVLIGVASSGVHSNGYSLVRKVLELTGLRLDSEAPFA
                     SGMPVGEALLTPTRLYAKSALAAIKLGGVKGLAHITGGGITGNLPRALPPGLDADVDL
                     GAWKLPPVFQWLAREAGLPEAEMLRTFNCGIGLIVVADATKAADVAMAFRDYGEQAFP
                     IGALVTSSGPEPDVRYRGRLLP"
     gene            complement(2054..2620)
                     /locus_tag="VGM72_03550"
     CDS             complement(2054..2620)
                     /locus_tag="VGM72_03550"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEY4264367.1"
                     /translation="MATTIALAAGIEISSAQTTPRALPNLQNQQQEQTPSPNSDNGQY
                     GGRSDFRDGHGFGNAGDRLDRRLAFLHTRLRITAAQERTWMVFAAVLKDEAQDGGRDR
                     GRDANDRRAPSVVERLEERQHRLANRGAELDHVIGALQPLYASFSEEQKHTADRLMFH
                     PNRERGFGGHRRADTRGDRFDRQDHDYR"
BASE COUNT          516 a          885 c          855 g          502 t
ORIGIN      
        1 ggcgcaggcc gaggcgttct atgcggtcca cagcgcgcgt ccgttcttca acggcctggt
       61 cgcgttcatg acctcgggtc cggtggtcgt tcaggtgctg gaaggcgagg gcgcggtcct
      121 caagaatcgc gaagtgatgg gggccaccga tccggccaag gcggcaccgg gcaccatccg
      181 caaggatttc gccgaaaata tcgaggccaa ttccgtgcat ggctcggatg cgcccgacac
      241 cgcggccaac gagattcgct tcttcttcag cgatctcgaa atcaccggct gatcgaaccc
      301 gccactatct tgaccgcttg cgggtagagc atgtgctcgg cttcgagcac gcgcgcggcc
      361 agagtctcgg gcgtgtcttc cagctgcaca ggcaccgagc tttgcaggat cgcgggtccg
      421 gaatccaatt ccggaatcac atagtgcacc gtgcagccgg acatcttttc gccggtggca
      481 agtacgcgtt catgcacatt ggtgcccttg aacgcaggca gcagcgaggg atggatgttg
      541 atcagccgcc cctgccacgc gcgtatgaag ccatcgctca ggatgcgcat gaaaccggca
      601 aggcagatca attctacgcc ggcatcgcgc aagcgcttgt ccatcgcggt atcgaatgcc
      661 tcgcgggacg caaacgcttt gtgggaaatc acaaccgttg gaatgtgagc gtcggacgcg
      721 aacttcagtc cgcccgcatc cgcgacatta gagatcacag ccacgatttc ggccggatag
      781 tcacgtgccg cgcaagcatc gatcagcgct ttcagattgc tcccccggcc tgagatcagc
      841 accgcgattc tgagccggcc ggcactcacg gcagcaggcg gccgcgatag cgcacgtccg
      901 gttcgggacc gctggaggtg acgagcgcgc cgatcggaaa tgcctgttcg ccataatcgc
      961 gaaacgccat cgcgacatcg gcggccttgg ttgcgtccgc gacaacgatc aatccgattc
     1021 cgcaattgaa ggtgcgcagc atttcggctt ccggcagacc ggcctcgcgc gcgagccatt
     1081 ggaatacggg gggtagtttc cacgccccta aatccacatc cgcatcgagg cctggcggca
     1141 gggcgcgcgg cagattgccc gtgatgcctc cgccggtgat atgggcgagc cctttgacac
     1201 ccccgagctt gatcgccgca agcgccgatt tcgcatagag ccgggtcggc gtgagcaacg
     1261 cttcgccaac aggcatgccg ctcgcaaatg gcgcttccga atccagacgc aggccggtca
     1321 attccagaac cttgcgcacc agcgaatagc cgttggaatg aacccccgat gaggccacgc
     1381 cgatcagcac atcgcccgcg cgtatggccg ccaggtcggg aaggacctgg ccgcgctcga
     1441 ccgcacccac ggcaaagccc gccaggtcga aatcgccctt ggcataaagg ccgggcattt
     1501 ccgcggtttc accgccgatc agcgcgcagc ccgattcctt gcaggcgcga gcgatgcctt
     1561 cgatcacgat agcggcttcg tccacgtcga gccggctgct cgcataataa tccaggagga
     1621 acagcggctc ggccccttgc accacgatgt cgttgacgac catcgccaca aggtcctgcc
     1681 cgatggtgtc gaatttcttc gcctcgatgg cgagcttcag cttggtgccg acgccatctg
     1741 tggaggcgac cagcagcggg tccttgaatc cggccgcctt aagatcgaac agcccgccaa
     1801 aaccgccgag cgcggcatcg gcgcccgcgc gtctcgtagc cttggcggcg ggggcaatgc
     1861 ggtccaccaa ggcatcgcct gcgtcgatgt cgacgccgga atccttgtag gtaacggagg
     1921 gtttgccggt gctcatatat ccccgagagc gggatgcggg ccatgccgcg atgggccatg
     1981 ctgtagtgag cgccgcgaaa gcgtgcaaga gccgatgcgc cgctgcaacc ggcagcggcg
     2041 catctctcaa caactaacga taatcgtgat cctggcgatc gaagcggtcg ccgcgggtat
     2101 cggcccgccg atgaccgccg aaaccgcgct ctcggttcgg atggaacatc aaccggtcag
     2161 cggtgtgttt ctgctcctcg ctgaaggagg catacaaggg ttgtagcgcc ccgattacat
     2221 ggtccaactc ggcgccgcga ttcgccaacc tatgctggcg ctcttcgagc cgttcgacca
     2281 cgctgggtgc gcgccggtca ttggcatcgc gtccgcgatc gcggcctccg tcctgggcct
     2341 cgtctttcaa tacggcggcg aacaccatcc aggtgcgctc ctgtgctgct gtgatgcgca
     2401 gcctcgtgtg aaggaacgcc agccgccggt ccagccggtc gcccgcattg ccgaatccgt
     2461 ggccgtcgcg gaaatcggag cggccaccat attggccgtt gtcgctgttg ggagaggggg
     2521 tctgctcctg ttgctgattt tgcagattgg gtaaggcccg gggggttgtt tgggcggaac
     2581 tgatctcaat tccggcggcc agggcaatcg tcgtggccaa tcccagccca gatgctgcgc
     2641 gccaggaaat tatgcgactc atgttttacg ctccatgggt aatcggcgcc tttgaacgcc
     2701 gagcacccct ccgcgcacaa atctagtggc cgtgagctgt cgccacgatg aacaaaaa
//
LOCUS       DASYEG010000093        10568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_317852, whole genome shotgun sequence.
ACCESSION   DASYEG010000093 DASYEG010000000
VERSION     DASYEG010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 10568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10568
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317852"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VGM72_03555"
     CDS             complement(<1..683)
                     /locus_tag="VGM72_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY4264368.1"
                     /translation="MTPALRLADVPNLPMPTGEELMKYLLSTALITLAMSGYASAAEV
                     TLVAPGGIRAAVEQMIPAFEKKTGHKVKATFGSGNGTKAQIVKGDAFDVPIVQPPYPD
                     VLTSGNVVTASETPLAHVAVGLAVRPGTPHPDISTPDAVKKLFLSAKSISYPDPKGGA
                     AAGVSMNETLKKLGISDQMQSKIKLAQGGAGAMKMLASGEVEFGLTFVSEIITEPGVE
                     AVGPLPESIS"
     gene            835..1935
                     /locus_tag="VGM72_03560"
     CDS             835..1935
                     /locus_tag="VGM72_03560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4264369.1"
                     /translation="MFRMAGWYDWPTSCSFKSTAAISTCLVLVIGMVDYLSGWQVSWA
                     AFYVYPVAIAAWYVSARWAYGLSLLSVLLFTAGDVAAGFQFLSWVILVWNALTRLIFN
                     AIVIELLVHVRSLTHGLEMRVMERTIDLRKEIAERERLERELLEVGARERRQLGFDLH
                     DGLCQHLTGTALAVQVLKEKLTHKGAPESAEAAKAVDLIEDGIALSRKLAKGLQPVEM
                     HAGGLMQALQEFAAATAELFKVSCRFECDAPLLLSDVSTADHLYHLAREATMNAIKHG
                     RAKNIVIALEERDEGSFLAVTDDGSGIPDPLPKNRGIGLTIMAQRAKLIGASFAIQAT
                     TPQGTVVVCCLPDALPKAEQIYDVPKRDPHYV"
     gene            1928..2581
                     /locus_tag="VGM72_03565"
     CDS             1928..2581
                     /locus_tag="VGM72_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4264370.1"
                     /translation="MSERKHEILLVDDHPLVRESLASLINQQPDLMVCGEAESVKQAL
                     EYLSKASPALAIIDLSLKDSSGLDLIKAMKMAYPQIGMIVLSMHDERHYAERAIRAGA
                     RGYVMKRETTKKIIAAIHQVLAGRLGVSEEVAALFAEKFLDNQRSGTPVEELSDRELE
                     VFRLLGAGLDTRSVATRLQISIKTVQAYCARIKDKLNLANATELLREAIRWNESEIL"
     gene            2595..3074
                     /locus_tag="VGM72_03570"
     CDS             2595..3074
                     /locus_tag="VGM72_03570"
                     /inference="COORDINATES: protein motif:HMM:NF001095.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEY4264371.1"
                     /translation="MTEIFVDGDACPVKDEIERVADRHGLNVHLVTNGGVRPSRNPRV
                     RHVIVQEGADAADDWIAQHIGPCDIAVTADIPLASRCLTAGAAVLGPSGKKFTSETIG
                     MALGMRDLHRHLREATGQQTFNRGFTKQDRSRFLGALENEIQAMKRNASDAAKSPQT"
     gene            3302..4546
                     /locus_tag="VGM72_03575"
     CDS             3302..4546
                     /locus_tag="VGM72_03575"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY4264372.1"
                     /translation="MTHPTFTTLGLTEALRRALTAENYSVPTPIQAQAIPLILAGRDV
                     LGIAQTGTGKTAAFGLPLMQRLVENHSAAAPNTTRALILAPTRELAIQIDESLRSYGR
                     HLKLRHAVILGGVNQNRQVQALRHGVDVLVATPGRLLDLIQQKHLRLNAVSELVIDEA
                     DRMFDMGFIRDVRRIVAYLPRQRQSMLFSATMPPEVSHLVAEILRDPARIDISPAAVT
                     ADNIDQRVYFVPAQEKRTLLQNLLSDAAMKRVIVFTRTKHGANKVAEHLEKAGHAAEA
                     IHGNKSQNARQRALEEFRTGRARILVATDIAARGIDIDDVTHVVNFELPDVAESYVHR
                     IGRTARAGTVGVAIAFCDPAERDSLRAIERLVKQPLTTMGGDRNASGDRHAAAAKPGR
                     GHAPKHSHQGPRGNRRNARRAA"
     gene            4562..4783
                     /locus_tag="VGM72_03580"
     CDS             4562..4783
                     /locus_tag="VGM72_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264373.1"
                     /translation="MAMKKKKSLPAPYAALTKDMRFEGTYEVLVPAPERVKPHRVPLQ
                     FETLQQAESWIHSPEGVEMIEDLLSERQK"
     gene            complement(4803..6260)
                     /locus_tag="VGM72_03585"
     CDS             complement(4803..6260)
                     /locus_tag="VGM72_03585"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4264374.1"
                     /translation="MDSLRPVHLTLLASLVFAALGVAPAQSQPQRIILYEGARLIPGD
                     GSAAIENSAFLVTGTQFTRVGRKGEVALPAGATRVDLTGKTVMPTLVDMHGHLGFQNI
                     ADGTMSKESFTRENLIDHLQRFAYAGVGAIIGIGDLVERSDMHGGRTKWGDVPLRVRN
                     EVIPGAALYKTAGAGMAWPGSGAQGHPSRVDVSYPVSTPEEARAAVRDYVRMKPEFIK
                     IWVDDREGTKKTLTPALYGAILDEAHKFNVPVGVHNVKLSDAKLLMKGGVEGWLHIPV
                     RGGDVVDAEIIGIVKDRIARNDRPNMWVTPALITAWMNTQGGGRPVWLDDPLLKALYS
                     PANIEEYWGAPLKKMTPEQVARAKQAFMADGKNMMLLRAAGMKVVGGTDTGQTRHFMA
                     FFNHLDLESMVAMGLTPNEAIKAATSDGAAMGHFNTGLVAPGRNADFIVLDANPLDSI
                     SNTRKIDKVYLRGDEVPRAQYAAKWQAKFKTAAAH"
     gene            complement(6373..7302)
                     /locus_tag="VGM72_03590"
     CDS             complement(6373..7302)
                     /locus_tag="VGM72_03590"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY4264375.1"
                     /translation="MNGPIHRIVQTALWGVLLASPVSAQPSPGIPGVVAAGTAPELVS
                     EMFENTEGPLGAPDGSLYFSDTNASLTYHLDLNGKITIARFNTHRGNGIALTRDGDMV
                     WAEGDGPRLSTLNKVNGGYRSLLPGFRLFQPNDLIVDSKGGIYFTDPGPRPVVPGLKV
                     YVYYVPPGAKLPIVIDDSVPRPNGITLSPDGRTLYVDNTIGTGVFRYDVQPDGTVKNR
                     RPFAELHDIKPGEEGFGDGMGMDRAGRLYVTSLVGIQVFDPAGKYLGTIKVPRQPSNV
                     AFAGPDKKTLYISARQGLYRLQMLSQGPDRPGK"
     gene            complement(7333..8406)
                     /locus_tag="VGM72_03595"
     CDS             complement(7333..8406)
                     /locus_tag="VGM72_03595"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4264376.1"
                     /translation="MIEDTENPPQGEDHLNRRGLFQALAAGAAAAGAFPSQAFAQNQP
                     AIASTADYARDPNRWGSAEVAALFPGFQHLDMKTSGAIIRVRHGGSGPPLLLIHGNPE
                     SHMTWYKIAAKLSQRYHVVLPDLRGYGDSSLPEPGPNHINYSFRAFAQDNVEIMEQLG
                     YRRYFVAGHDRGGRTTFRMCLDHPERVIKATIMDVLPNYHVWTNTTKQWALASWHWLF
                     MAQPEPFPERQMSAAGAEFFLRNRMAFRGGTGLGFLTQTAFDEYVRAFTLKTITGSCR
                     DYRACATCDFEMDTADKDRKITTPLFILWGARGPERSDEFINIWRKFASNIEGYEGLP
                     CGHYMQEEVPDRVYDHFVKFFTA"
     gene            complement(8420..10108)
                     /locus_tag="VGM72_03600"
     CDS             complement(8420..10108)
                     /locus_tag="VGM72_03600"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY4264377.1"
                     /translation="MRSALMLTVSAGLLGFLTQSAYAQGGAALAGQVSSAKEGMMEGV
                     VVSAKKDGSTIAVSVVSDRAGRYAFPAGRLEPGHYTLKIRAIGYELDGASAADVKAGA
                     TASADIKLAPAKNLSAQLTNAEWLASAPGSDQQKKFLLSCNSCHSYQRIFNSQHDSDE
                     FMQVFDRMAGYYPGSTPLQPQRLVGTARRNLGGGMGNASGPGAARANVMKSAAEWLAS
                     INLSKGPERSYSYHTLPRPTGRATKMVVTEYDLPRKTIMPHDVMVDKDGMVWYSDFGA
                     LFIGKMDPKSGKVTEYPIPKIKEGFPVGTLDLEQDQEGNMWVAMMYQGGIAKLDTKTG
                     KVQTWSVPKEWQTDATQQSFASPNSLHVDGKVWVKNSDGAQIMRVDVKTGQWENFGAF
                     VDPDTKRPIGSYGINADRNNNLYLLDFNANNIGVLDGVTKKLEVHKTSIQNSRPRRGR
                     VDEQNRLWFAEYDGNAIGMYDPKSKSIKEWVVPTPWSAPYDVVVDKNGDAWTASMMTD
                     RVTRINTKTGEQTEYLLPNPTNIRRVWIDNSTTPVSFWVGSNHGASIVKLEPLE"
     gene            complement(10305..>10568)
                     /locus_tag="VGM72_03605"
     CDS             complement(10305..>10568)
                     /locus_tag="VGM72_03605"
                     /inference="COORDINATES: protein motif:HMM:NF015602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HEY4264378.1"
                     /translation="GIPDPATNLTPDQRVEVLRGAVEKLIKKSLPANSKLQIEQDKIS
                     GTFIYRSINPDTGEVITQWPSEKLLELRDYLKEMEGVLVNKQV"
BASE COUNT         2038 a         3086 c         3236 g         2208 t
ORIGIN      
        1 gaaatcgatt ccggcaaagg tcccacggcc tcgacgcccg gctcggtgat aatttcgctg
       61 acgaatgtga gaccgaattc aacttcgccg gaagccagca tcttcatcgc gcccgcaccg
      121 ccctgcgcga gcttgatctt ggattgcatc tgatcgctta tgccgagctt tttcagggtc
      181 tcattcatgc tcacgccggc ggccgctccc cctttgggat ccggatagga aatggatttc
      241 gctgagagga aaagcttctt caccgcgtca ggcgtggaga tatcggggtg tggcgtgcct
      301 ggccgcacgg caagaccgac agcgacgtga gcgagcggcg tctcgctcgc ggtcaccaca
      361 ttgcccgagg tcagaacgtc cggatagggc ggctgaacaa tcgggacatc gaaggcatcg
      421 cctttgacaa tttgcgcctt ggtgccgttg ccggagccga aggtcgcttt gaccttgtga
      481 ccggttttct tttcgaacgc cggtatcatc tgctccaccg cggcccggat accgcccggt
      541 gcgacgagcg tgacctccgc ggcgctcgca taaccggaca tcgccagtgt gatgagagcc
      601 gtagacagca gatatttcat gagttcctcc ccggtgggca tcggcagatt cggaacgtcc
      661 gccagacgca gtgccggggt cattctatcc gaggccggga tgccgcgtcg atggctcgca
      721 aaggagagtg cgaccctccg ttttgaacac cggaaagcgt atgttttaca cccggcaaat
      781 gctaaacctg aacccacgat aaaacgccgg accgcagagt caaaaggggg cgccatgttc
      841 cgcatggcag ggtggtatga ttggcctacg tcgtgctcgt tcaaaagcac ggcggcgatt
      901 tcaacgtgcc tggtcctggt aatcggtatg gtcgattacc tgtcgggatg gcaggtttcc
      961 tgggccgctt tctacgttta tccggtcgcg atcgccgcgt ggtacgtcag tgcccgctgg
     1021 gcgtatgggc tctcgctttt gagcgttctg ctgttcacgg caggggacgt ggcggcgggt
     1081 tttcagtttc tgagctgggt gatcctggtg tggaacgctc tgacgcggct catattcaat
     1141 gcgatcgtga tcgagcttct ggtccatgtc cgttcgctga cgcatggcct cgagatgcgg
     1201 gtgatggaac gcacgatcga tttgcgcaag gagatcgccg aacgcgagcg tctggagcgc
     1261 gaacttctgg aagtgggcgc gcgggagcgc cgccaattgg gcttcgatct tcatgacggt
     1321 ctctgccagc acctgacggg aacggccctt gccgtgcagg tgctgaagga aaaattgact
     1381 cacaaaggcg cacccgaatc cgctgaggcc gccaaagcgg tcgacctcat cgaagacggc
     1441 attgcgttgt cgcgcaaatt ggcaaagggc cttcaacccg tcgaaatgca cgccggcgga
     1501 ctcatgcagg cgctgcagga atttgccgcc gcgaccgcgg agctgttcaa ggtttcctgt
     1561 cgtttcgaat gcgacgcgcc gcttttgctg agcgatgtct cgacggccga tcatctttat
     1621 catctggcgc gcgaagcgac gatgaacgcc atcaaacatg gccgcgccaa aaatatcgtg
     1681 atcgcgctcg aggaacgtga tgaaggatcc tttttggcag tgaccgatga cggtagcgga
     1741 attcccgatc cgcttcccaa gaatcgtgga atagggctga cgatcatggc tcagcgcgcc
     1801 aaactgattg gtgcgagttt tgccatacag gcgacaaccc ctcaggggac cgttgtcgtc
     1861 tgctgtttgc ccgatgcctt gccgaaggcg gagcagattt atgacgtacc aaagcgcgat
     1921 ccgcattatg tctgaaagga agcatgaaat attgctggtt gacgatcatc cgctggtgcg
     1981 ggaatcgctc gcgagtctga tcaatcagca accggacctg atggtctgcg gtgaggccga
     2041 gagcgtgaag caagccctcg agtatttgtc caaagcatcg ccggcccttg cgatcatcga
     2101 tctttccctg aaagacagtt cggggcttga tctgatcaag gccatgaaaa tggcttaccc
     2161 ccagatcggt atgattgtgc tttcgatgca tgacgaaagg cactacgccg aacgggctat
     2221 ccgcgcgggc gcgcgcggtt atgtcatgaa gcgcgaaacc acgaaaaaga tcatcgcggc
     2281 gattcatcag gttctcgccg gaagattggg cgtgagtgag gaagtggcgg cgttgttcgc
     2341 ggagaaattc ctcgacaatc agcgctccgg aacgcccgtg gaagaattga gcgaccgtga
     2401 attggaggtc tttcgtctgc tgggcgcggg cctggacacg cgcagcgtcg ccacacgcct
     2461 acagatcagc atcaagacgg ttcaagccta ttgcgcgcgt atcaaggaca agctcaatct
     2521 tgcgaacgca accgagctgt tgcgcgaagc aattcgctgg aacgagagtg aaatcctctg
     2581 agggcatttc ctgcgtgact gagatttttg tcgatggcga tgcgtgcccc gtcaaggatg
     2641 aaatcgaacg cgtcgcggac cgtcacggcc ttaatgtcca tctcgtcacc aatggcggag
     2701 tgcggccgag ccggaatcct cgcgtgcgcc atgtgatcgt gcaggaaggg gccgatgccg
     2761 ccgatgattg gatcgcgcag catatcgggc cgtgcgatat tgcggtcacc gccgacattc
     2821 cgcttgcttc gcgctgtctg acggctggcg ctgctgttct cggcccttcg ggcaagaaat
     2881 tcacctccga gaccattggg atggcgctcg gcatgcgcga ccttcatcgt catttgcgcg
     2941 aagcgacagg gcagcagacc ttcaatcgcg gcttcacgaa acaggaccgt tcgcgattcc
     3001 tcggcgcgct ggaaaatgag attcaggcga tgaagcgcaa cgcgagcgat gcggcgaaat
     3061 cgccgcaaac gtaaagcaaa gcttaacggc gcagcaggcc atttcaggcc tggggttgac
     3121 ttcgaatccc gtacctcata taggcagcgc accctcgcgg tcgcacgacg gcgccgatta
     3181 catggagcgc ctgtcaccgc gggatggttt tcaacatacc gttcgctcca gatgtgcgtg
     3241 gaagacggca accgccgacg accgcgcgat tctcgccgtt ctgatgcgat aaggaaaaat
     3301 tgtgactcat ccgactttta cgaccctcgg tctgaccgag gcgttgcggc gcgcgctcac
     3361 ggccgagaat tacagcgtgc cgacgccgat ccaggcgcag gccatccccc tcattcttgc
     3421 cggccgcgat gttctgggca tcgcgcaaac cggcaccggc aaaaccgctg cgttcggtct
     3481 gccgttgatg cagcgcctgg tggagaatca cagcgctgcc gcgcccaata ccacacgcgc
     3541 gctgatcctc gcgccgaccc gcgagcttgc cattcagatc gacgagtcct tgcggtccta
     3601 tggccgtcat ctgaaattgc gccacgccgt cattctcggt ggcgtcaatc agaacagaca
     3661 ggttcaggcg ctgcgtcatg gcgtggacgt tctggtcgcg acgcccggcc ggctgctgga
     3721 cctgattcaa caaaaacatc ttcgtttgaa tgcggtcagc gaactggtca tcgatgaagc
     3781 cgatcgcatg ttcgacatgg gattcatccg ggacgtgcgg cgaatcgttg cctatctgcc
     3841 gcgccagcgg caatcgatgc tattctccgc gaccatgccg cctgaagttt cgcatctcgt
     3901 cgcggaaatc ctgcgcgatc cggcgcgcat cgacatttca cccgccgccg tgacagccga
     3961 caacatcgat cagcgggttt acttcgtacc cgctcaggag aagcgcacgc tgctgcagaa
     4021 tcttttgagc gatgcggcga tgaagcgcgt gattgttttc acccgcacca agcatggcgc
     4081 caacaaggtg gccgagcatt tggaaaaggc gggtcatgcc gccgaggcca tccacggcaa
     4141 caaatcccaa aatgcgcgtc aacgtgcgct ggaagaattc cgcacgggcc gcgctcgcat
     4201 cctggtggcg acggatatcg ctgcacgcgg catcgatatc gacgatgtca cgcatgtggt
     4261 gaatttcgag cttccggatg ttgccgaaag ctatgtccat cggattggcc gcacggcacg
     4321 cgccggcacc gtcggggtgg ccatcgcgtt ctgcgatccc gccgaacgcg attccttgcg
     4381 tgcgatcgag cggctggtga agcagcctct caccacgatg ggcggagacc gtaacgcaag
     4441 tggcgaccgc cacgcagcgg ctgccaagcc ggggcggggg cacgcgccca aacattccca
     4501 tcaaggcccg cgcggcaatc gccgcaatgc ccgccgcgcg gcctgatcaa aggagacgag
     4561 catggcgatg aaaaagaaga aatctttgcc cgcgccgtac gccgccctta ccaaggacat
     4621 gcgcttcgaa ggcacctatg aagtgctcgt accggcgccc gagcgggtga agccccatcg
     4681 ggtgccgctg caattcgaaa cgctccaaca ggcggagtcc tggatccata gcccggaagg
     4741 cgtggagatg atcgaggatt tgctgagcga gcgccagaaa tagcgcgttg ttctaaccct
     4801 cgtcagtgtg ccgcggcggt cttgaacttg gcctgccatt tggcggcata ttgcgctcgg
     4861 ggcacttcat cgccgcgcag atagaccttg tcgatcttgc gcgtgttgga aatgctgtcg
     4921 agcgggttcg cgtccagcac gatgaaatcg gcattgcggc caggtgccac caatccggtg
     4981 ttgaaatgcc ccatggccgc gccgtcgctg gttgcggcct tgatggcttc gttcggcgtc
     5041 aggcccatcg cgaccatgct ttcgagatcc agatggttga aaaacgccat gaaatggcgc
     5101 gtctggccgg tgtcggtgcc gccgacgact ttcatgccgg cggcgcgcag cagcatcatg
     5161 ttcttgccgt ccgccatgaa ggcttgtttt gcgcgggcga cctgttcggg ggtcatcttc
     5221 ttcaggggcg cgccccaata ttcctcgatg ttcgcaggcg aatagagcgc cttgagaagc
     5281 gggtcgtcga gccaaacggg gcgcccgccg ccttgcgtgt tcatccacgc ggtaatgagt
     5341 gccggagtga cccacatatt gggccgatcg ttgcgcgcga tgcgatcctt gacgatgccg
     5401 atgatctcgg cgtcgaccac gtcgccgccg cgcacaggga tatgcagcca tccctccacc
     5461 ccgcccttca tcagcagttt ggcgtcggag agtttcacat tgtgcacacc gaccggcacg
     5521 ttgaatttat gcgcctcatc caggatcgca ccgtagagcg cgggcgtcag cgtctttttg
     5581 gtgccctcgc gatcgtccac ccagattttg atgaattccg gtttcatccg cacatagtcg
     5641 cgcaccgccg cgcgggcttc ttccggtgtg gacaccggat aggacacgtc gacacgggag
     5701 ggatggccct gcgcgcccga tccaggccag gccatgcccg cgccggcggt cttgtaaagc
     5761 gcagcacctg gaatgacctc gttgcgaacc cttaacggta catcgcccca tttggtccgt
     5821 ccgccatgca tgtccgagcg ctcgaccagg tcgccaatgc cgatgatcgc gcccacgccg
     5881 gcataggcaa agcgctgcag atgatcgatc aggttttcac gcgtgaagct ttccttggac
     5941 atcgtgccgt ccgcgatatt ctgaaagccg agatggccat gcatgtcgac cagcgtcggc
     6001 atcaccgtct tgccggtgag atcgacgcgc gtagcgccgg cgggtagagc cacttcgcct
     6061 ttgcggccga cccttgtgaa ttgcgtgccg gtcacgagga aggcggaatt ttcgattgcc
     6121 gcgctcccat cgcccgggat caaacgcgca ccctcataaa ggattatccg ctgcggctgg
     6181 ctttgcgccg gagcaacgcc gagcgctgcg aagacgagcg acgcgagaag ggtgagatga
     6241 accggcctca aagagtccat tgcgttcccc cgggacgagt ttgtcttttc tgcgcacaag
     6301 gatgcgtctt tccgcgcccg ctggcaacca aatcgaagct gccccggcgt ttgcggattc
     6361 gggggcgaac gctcacttgc cggggcggtc gggtccttgg gacagcatct gcaagcgata
     6421 cagcccctgg cgcgcgctga tgtagagcgt cttcttgtca ggacctgcaa aggcgacatt
     6481 cgagggctga cgcggcactt taatggtacc gagatatttg cccgcagggt cgaaaacctg
     6541 aatgcccacc aggctcgtga cgtataggcg accggcgcgg tccattccca ttccgtcgcc
     6601 gaagccttct tcgcccggct tgatatcgtg cagctcggca aaggggcgcc ggttcttgac
     6661 cgtgccgtcg ggctgcacgt cgtaacggaa cacaccggta ccgatggtat tatcgacgta
     6721 gagcgtgcgg ccatcgggcg agagcgtgat cccattcggg cgcggcacgg aatcgtcgat
     6781 gacgatcggc agcttggcac cgggcggaac gtaatagaca tagactttca gcccgggcac
     6841 cacggggcgc gggcctggat cggtgaaata gatgccgcct ttggaatcga cgatcaggtc
     6901 gttgggctgg aacaggcgaa aaccaggcag caggctgcgg taaccgccat tcactttgtt
     6961 cagagtactg agacgcggtc catcgccttc ggcccacacc atgtcgccat cccgcgtcaa
     7021 cgcgatgccg ttgccgcgat gagtgttgaa gcgcgcgatg gtgatcttgc cgttcaggtc
     7081 gaggtgatag gtcaggctcg cattggtgtc ggagaaatag agacttccat ccggagcgcc
     7141 gagcggcccc tcggtgtttt caaacatctc gctcacaagc tcaggcgctg tacccgccgc
     7201 gaccacgccg ggaatgccgg gcgaaggctg cgcggaaacg ggggaagcga gaagcacgcc
     7261 ccaaagtgcc gtctgcacaa tccgatgaat tggtccgttc attcgccgca tgaatccccg
     7321 ccctccttgc gctcaagccg tgaaaaattt gacgaagtga tcgtagacgc ggtctggaac
     7381 ttcttcctgc atgtagtggc cgcaaggcag accttcgtaa ccttcgatgt tgctcgcgaa
     7441 ttttcgccag atgttgatga attcatcgga gcgttccggc ccacgcgcgc cccagagaat
     7501 aaagagcggc gtcgtgatct tgcgatcctt gtcggcggtg tccatctcga aatcgcatgt
     7561 cgcgcaggcg cggtagtcgc ggcaggaccc cgtgatcgtc ttcagcgtga aggcccggac
     7621 atattcgtcg aatgcggttt gggtgagaaa tcccaacccg gtacccccgc gaaaggccat
     7681 gcggttgcgc agaaagaatt ccgcgcccgc agcactcatc tggcgttcgg gaaacggctc
     7741 cggctgcgcc atgaacagcc aatgccagga cgccagcgcc cattgcttcg tagtattggt
     7801 ccagacatga tagttcggca gcacgtccat gatcgtggcc ttgatgacgc gctcgggatg
     7861 gtcgagacac atgcggaatg tcgtgcgtcc gccacgatcg tggccggcaa cgaaatagcg
     7921 gcgatagccg agctgctcca tgatctcgac attgtcctgc gcgaaggcgc ggaaactgta
     7981 attgatgtgg ttggggcctg gttcggggag cgatgagtcg ccataaccac gcaaatccgg
     8041 caatacgacg tggtagcgtt gcgaaagctt ggcggcgatc ttgtaccacg tcatgtggct
     8101 ttcgggattg ccgtggatca aaagcaaagg cggacctgag ccgccatggc gcacacggat
     8161 gatcgcgccg ctcgtcttca tgtcgagatg ctggaaaccc ggaaacagag cggcaacctc
     8221 ggcgctgccc catctgttcg ggtcgcgcgc ataatcggca gtggaggcga tggccggttg
     8281 attctgtgca aaggcttgcg aaggaaatgc gcccgccgcg gcagcaccgg cagcaagcgc
     8341 ctggaacagc ccacggcggt tgagatggtc ttcgccctgg ggtgggttct ctgtatcttc
     8401 aatcattgtg taatcagtcc tattccagtg gctccagctt cacgatcgag gcgccgtgat
     8461 tgcttccgac ccagaagctg accggggtcg tggaattatc gatccagacg cggcggatat
     8521 tggttggatt ggggagaaga tattcggtct gctcaccggt cttggtattt atgcgggtca
     8581 cgcggtcggt catcatcgat gcggtccatg cgtcgccatt cttgtcgacg acgacgtcat
     8641 agggcgcgga ccacggcgtc ggcacgaccc attccttgat ggatttggac ttcgggtcgt
     8701 acatgccgat cgcgttcccg tcatattcgg cgaaccacag acggttttgt tcatcgaccc
     8761 ggcccctgcg cggacgagaa ttctggatgc tcgtcttatg gacctccagc ttcttcgtaa
     8821 cgccatcgag gacgccgatg ttgttggcgt tgaagtcgag cagatagaga ttgttgttgc
     8881 gatcggcatt gatgccatag gagccgatcg ggcgtttcgt atccggatcg acgaaggccc
     8941 cgaaattctc ccactgcccc gtcttcacat cgacccgcat gatctgggcg ccgtcggaat
     9001 tcttgaccca gaccttgccg tcgacatgca gcgaatttgg cgacgcgaag gattgttgcg
     9061 tcgcgtccgt ttgccattcc ttgggtaccg accaagtttg aacttttccg gtcttggtat
     9121 cgagtttcgc gatgccgccc tgatacatca tggcgaccca catattgcct tcctggtcct
     9181 gctcgagatc gagagtaccg accgggaaac cctccttgat cttggggatc ggatattcgg
     9241 tgaccttgcc gcttttggga tccatcttgc cgatgaacag cgcgccgaaa tcggagtacc
     9301 agaccatgcc gtccttatcg accatgacgt cgtgaggcat gatagtcttg cggggcagat
     9361 catattcggt gacgaccatc ttggtcgcgc gccctgtggg gcgcggcagc gtgtggtacg
     9421 aatagctccg ttcgggaccc ttgctgagat tgatgctggc gagccattcg gcggctgact
     9481 tcatcacatt ggcgcgcgcg gcgccggggc cgcttgcgtt gcccatgcca ccgccgagat
     9541 tgcgccgtgc ggtgccgacg agacgctggg gttgcagcgg cgtgctgccc ggatagtacc
     9601 cggccatgcg gtcgaagact tgcatgaact catccgagtc gtgttgggaa ttgaagatgc
     9661 gctgatagga gtggcaggaa ttgcacgaca gcaggaattt cttctgctgg tcgctgccgg
     9721 gcgcactcgc gagccattcc gcattggtga gttgcgctga aaggtttttc gccggcgcga
     9781 gcttaatatc tgcgctggct gtggcgccgg ccttcacatc ggccgccgat gcgccatcaa
     9841 gctcgtatcc tatggcgcgg atctttagtg tgtaatggcc cggttcgagt cttccagcgg
     9901 ggaatgcgta acgtcccgct ctgtcgctca cgacggagac cgcgatggtg ctgccgtcct
     9961 tcttggcgct gacgaccacg ccttccatca tgccttcttt ggcggaactg acctgccccg
    10021 cgagtgccgc gcccccttgc gcataggcgc tttgcgtcag aaagccgagc aagccggcgg
    10081 aaacggtgag catgagagcg cttcgcatga caacctccct gaggggttta cacgcgtttg
    10141 cgcgcggttc gccccgcatt gccaatgatc gagcgcaagg gtgcgctttt ccacatttcc
    10201 cagcaagacg gattggtggc tgaatcaggg aatggaaggg caattcctgc gtttgttggg
    10261 tgtttgggtg ggctgccagg actatgagtc agccattcct gggcttacac ctgtttattg
    10321 accaacacgc cttccatctc tttcaaataa tctcgaagtt cgagcaattt ttcggacggc
    10381 cattgggtta tgacctcgcc tgtgtcggga ttgatggaac gatagataaa tgtgccgctg
    10441 atcttgtcct gttcgatctg aagtttggaa ttcgccggca ggcttttctt gatcagcttc
    10501 tcgacagcac cccgaagaac ttcgaccctt tgatcgggcg tgagattggt ggccggatcg
    10561 ggaatgcc
//
LOCUS       DASYEG010000094         5913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3508919, whole genome shotgun sequence.
ACCESSION   DASYEG010000094 DASYEG010000000
VERSION     DASYEG010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3508919"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VGM72_03610"
     CDS             <1..462
                     /locus_tag="VGM72_03610"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY4264379.1"
                     /translation="PRNAPYQAYRARDRYFVIAAGNDKLWASVCDIVERPGLVSDPRF
                     ATVKDRAKNQVELAQLLTAIFDARDAADWLAAFERVGVPASLINSYSESLADPQVQAN
                     DWIRELRLPGGAVTRTFASPLAIGGRHLPLPKAPPRLDEDRAAILAMIGER"
     gene            600..1526
                     /locus_tag="VGM72_03615"
     CDS             600..1526
                     /locus_tag="VGM72_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4264380.1"
                     /translation="MQTILSIANLSKTYASGFNALKSINLDIRKGEILALLGPNGAGK
                     TTLISIVCGIVNSSAGKVMVNGHDNLTDYRAARSLIGLVPQELTTDAFETVWATVSFS
                     RGLFGKPANPDYVAKVLKDLTLWDKKDSKIRTLSGGMKRRVLIAKALAHEPQILFLDE
                     PTAGVDVELRKDMWRIVRRLRESGVTIILTTHYIDEAEEMADRIGIINRGELILVEDK
                     AELMRKLGKKQLTLNLHEPLDVVPSSLAAYGLERNAGGNELVYTYDTKSERTGITALL
                     KDLAEAGIRFNDLNTTQSSLEEIFVSLVRRPS"
     gene            1523..2284
                     /locus_tag="VGM72_03620"
     CDS             1523..2284
                     /locus_tag="VGM72_03620"
                     /inference="COORDINATES: protein motif:HMM:NF011648.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4264381.1"
                     /translation="MNFRAVQAIYTFEMARAWRTVAQSIIAPVISTSLYFVVFGAAIG
                     GRISQIDGVSYGAFIVPGLVMLSLLTQSTMNASFGIYFPRFTGTIYELLSSPVSTIEI
                     VLGYVGAAASKSIILGAIILLTAGLFVPLHIAHPFWMLAFLLLTALSFSLFGFILGIW
                     ADGFEKLQIVPLLIITPLTFLGGSFYSIKVLPPFWQKVSLVNPVVYLVSGFRWSFYDI
                     SDVSVEWSLAMTAIFLAVCLILVWGIFKTGYRLKS"
     gene            complement(2411..2713)
                     /locus_tag="VGM72_03625"
     CDS             complement(2411..2713)
                     /locus_tag="VGM72_03625"
                     /inference="COORDINATES: protein motif:HMM:NF037081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY4264382.1"
                     /translation="MAAAVKAKVETVSSKQLAQQLAEKHEISKKQAAMIMDDLSGFIT
                     KHLKKGVRIRLNGLGILQVRKRAARMGRNPATGEAIKIKASKKVAFRVAKELKTSV"
     gene            2985..4793
                     /locus_tag="VGM72_03630"
     CDS             2985..4793
                     /locus_tag="VGM72_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4264383.1"
                     /translation="MAAPLLLLQNIRLTLGRTALLDRAELSVGAGERLCLVGRNGSGK
                     STLLRIGAGLVEPDGGTRFLQPGATIRYLPQEPMLGDFSTVRSYVEAGLAPGDDPNRA
                     YYFLGKLGLTGDEPTATLSGGEARRAALTRVLASSPDILLLDEPTNHLDLPAIEWLEN
                     ELRAMRSALVLISHDRRFMENLGRATVWLDRGVTRRLEQGFAGYEIWRDEILEQEERE
                     RHKLDRRIAMEFDWVRYGVTARRKRNQRRMAELRTLRRERREQRHATGNVKFSPAESD
                     QSGSCVIEAKTIAKTYGAGPVVRDFSIRILRGDRVGIVGPNGAGKTTLLNLLTGRLEP
                     DSGTVKLGTNLRIATLDQSRALLDPEMSLSGVLTGGVGDIVHAGGQARHVISYMKDFL
                     FQPEQARTPVHVLSGGERARLLIAKALATPSNLLVLDEPTNDLDLETLDLLEEMIADY
                     RGTAVIVSHDRDFLDRVATSVLMAEGDGRFVEYAGGYTDMLAQRGEGVSAKEPPKPVD
                     SRAAAKERNPASAKRKLSFAEKHALDTLPSRIAALTGEIAALQSQLSDSALYARDPKR
                     FLETSAKLETSQNDLNAAEMRWLELEMLREDIEGAG"
     gene            complement(4794..>5913)
                     /locus_tag="VGM72_03635"
     CDS             complement(4794..>5913)
                     /locus_tag="VGM72_03635"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEY4264384.1"
                     /translation="RLINTAAEAAIVGGLADWFAITALFRHPLGLPIPHTALIPSRKN
                     EIGRALGQFVRDSFLDPALLTERLRRHNRAGQIAQWLDTEEAADFIAARTAALVPPLL
                     SGLNDADVHNFVRNLAQQGISRVEFVPIVDGLLDDFVGTGKHMDFIDAGVAMAGPSLQ
                     ALREPIVERVGERTGRFFPRYFDRRIAQAIVDGAQAWLNAIVTPGSDERTRLDCWMRD
                     SVMAFRRSADYPKLLAEIQNAVASNHALLNALGTIWDDVKRELIADTAGASPKTAIVT
                     VKIVRTFGRLLQQMPAMQDYLNAAIEGVLVDSIAPWRIQIGKYIAEIVESWDGPKVAA
                     TIESHVGGDLQYIRINGTLVGAAIGALLFLIGAGIGSL"
BASE COUNT         1150 a         1770 c         1762 g         1231 t
ORIGIN      
        1 ccgcgcaacg cgccgtatca ggcctatcgc gcgcgggacc gctatttcgt gattgctgcg
       61 ggaaacgaca aattatgggc cagcgtgtgt gacattgttg aaaggccggg actcgtgtcc
      121 gatccgcgct tcgccaccgt caaggatcgt gcgaagaacc aggtcgaact tgcgcaattg
      181 ctgaccgcga tcttcgacgc ccgcgacgcg gccgactggc tggcggcatt cgagcgggtg
      241 ggagtccctg cgagtctgat caacagctat tcggaaagcc ttgccgatcc gcaggtgcaa
      301 gccaatgact ggattcggga attgcgcctt ccgggaggcg ccgttacgcg gacctttgct
      361 tccccgctcg ccatcggggg gcgccatttg ccgcttccca aagcgccgcc gcgcctcgat
      421 gaagaccgcg ccgcgatcct ggccatgatc ggcgaacgct gattgcccgc aggacctcga
      481 aggttccaga agaaattttc gttttcaccc gccaacacat cgcatgaccg ccgagaattt
      541 ctcttgtcgg gaggggaaag ttctgctcgt ttgtcatccg ccgccaaacc atccgcttca
      601 tgcaaacgat tctttccatc gccaatcttt ccaagaccta tgcgtccggg ttcaatgctc
      661 tcaaaagcat caacctcgac atccgcaagg gcgagattct ggcgcttctg ggccccaatg
      721 gcgcgggcaa gacgacgctg atcagcatcg tttgcgggat cgtcaattcc agtgcaggaa
      781 aagtgatggt gaatggtcac gataatctta ccgattatcg cgcggcgcgc agcctgatcg
      841 gtctggtgcc gcaggaactg acgacggacg cttttgaaac tgtgtgggcg accgtgagtt
      901 tcagccgcgg cctgttcggt aagcccgcca atcccgatta tgtcgccaag gtgctgaagg
      961 atctcaccct ttgggacaag aaggacagca agatccgcac cctctccggc ggcatgaagc
     1021 ggcgcgtgct gatcgccaag gctttagcgc atgagccgca aatccttttt ctcgatgaac
     1081 ccactgccgg cgtcgacgtg gaattgcgca aagacatgtg gcgaattgtg cgccgtttgc
     1141 gcgaatcggg ggtcacgatc atcctgacga cacattatat cgatgaagcc gaagagatgg
     1201 cggaccgcat cggcatcatc aatcgcggtg agttgatcct ggtggaagat aaggccgaac
     1261 tgatgcgcaa gctcggcaag aagcaattga ctctgaactt gcatgagccc ttggatgtcg
     1321 tgccctcctc gcttgccgcc tatggattgg aacgcaacgc aggcggaaat gagcttgttt
     1381 atacctatga tacgaaaagc gagcgtaccg gtatcacggc gctactgaag gatcttgcgg
     1441 aagctggcat ccgattcaac gatttgaaca cgacccaaag ttcgctggaa gaaatcttcg
     1501 tcagtctggt gaggcgcccg tcatgaattt ccgcgcggtg caggcgatct acacattcga
     1561 gatggcgcgc gcctggcgga cggtcgcgca gagcatcatc gcgccggtga tttccacctc
     1621 gctctatttc gtggttttcg gggcggcgat cggcggtcgc atcagccaga tcgatggggt
     1681 gagttatggc gcgtttatcg tgccgggttt ggtgatgctg tcgcttctga cccaaagcac
     1741 catgaatgca tccttcggca tctatttccc gcggttcacc ggcacgatat acgagcttct
     1801 gtcctcgccc gtctccacga tcgagatcgt gctgggctat gtcggcgccg ccgcgagcaa
     1861 atccatcatc ttgggtgcga tcattctttt gacggcaggg ctattcgtgc cgcttcacat
     1921 cgcgcacccg ttctggatgc tcgctttcct cctgctcacc gcgctgagct tcagcctatt
     1981 cgggttcatc ctgggaattt gggcggatgg cttcgaaaag cttcagatcg tgccgctgct
     2041 catcatcacg ccgctgacct ttcttggcgg cagtttctat tccatcaagg tgctgccgcc
     2101 gttctggcag aaggtgagcc tcgtcaaccc cgtcgtttat ctcgtcagcg gcttccgctg
     2161 gagcttttac gatatttcgg acgtcagcgt ggaatggagc ctcgccatga cggcgatctt
     2221 cctcgcggtt tgcctgatcc tggtgtgggg gatattcaag accggctatc gcctgaagtc
     2281 ctgaattcgc ggttccgccg ctatggcggc aatacacaaa tccgcgcaaa gcgcggatac
     2341 gaaaaattac gaaggcggga agcttacaag gacggatagc aaacacgctt ccgtcctcgt
     2401 aaagcacgcg tcaaaccgaa gttttcagtt ccttggcgac acggaaggcg acttttttgc
     2461 tcgccttgat cttgatcgct tcgccggttg cagggttgcg gcccatgcgg gcggcccgct
     2521 tgcggacttg caggatacca agcccattca ggcgaatgcg cacgcctttc ttgagatgct
     2581 tggtgataaa gccactgaga tcgtccatga tcatcgcggc ctgcttcttg ctgatttcgt
     2641 gcttctccgc caattgctgg gcgagctgtt tggaactgac ggtttcaact tttgctttaa
     2701 ctgcggctgc cataggaaat ccctctgcga atcaccaagg aacgatacat attcgagcgc
     2761 tgtcattcga ctaattttca cacttgtgca ccattaggtg cgtattttcg ccctattccc
     2821 tgtgtttttc acatgttcgg gttgttcgcg ccccaaaata caagagaatt cgcatgccgg
     2881 tacgccattt tcttggacgt tttcattcgc cgcgggaggc tcgaacatcg gttgagaccg
     2941 tagattcgcc gttgtcatcc gctcgattgc cgggggtttg acagatggcg gcaccgcttc
     3001 tgctgctcca gaacatccgc ttaacgctgg gccgcaccgc gctcctcgat agggcggagc
     3061 tttcggtggg cgctggcgaa cgcctctgtc ttgtggggcg caacggttcg ggaaaatcga
     3121 cgcttctgag aatcggggcg ggacttgtgg aacccgatgg cggaacgcgc tttctgcagc
     3181 ccggcgccac gatccgctat ttgccccagg agcccatgct cggcgatttt tcaacagtgc
     3241 ggtcctatgt cgaggcgggc cttgcgccgg gcgacgatcc aaaccgcgct tattatttcc
     3301 tcggcaagct cggactgacg ggcgacgaac cgacggccac gctttcgggt ggcgaagcac
     3361 ggcgcgcggc gctgacacgg gttctggcgt cttcgcccga tatcctgctg ctggacgagc
     3421 cgaccaacca tctcgatctg ccggcaatcg aatggctgga gaatgagctg cgcgcgatgc
     3481 gttctgcgct cgtgctgatc agccatgatc gccgcttcat ggaaaatctt ggccgcgcca
     3541 ctgtttggct cgaccggggc gtgacgcgcc gcctggaaca gggcttcgcc ggatatgaaa
     3601 tctggcgcga cgagatactc gaacaggaag agcgcgaacg gcataagctc gatcgcagaa
     3661 tcgccatgga gttcgattgg gtacgctatg gcgtgactgc gcggcgcaag cgcaaccagc
     3721 gccgaatggc ggagctgcgg acccttcgcc gtgagcgccg cgagcaacgt cacgccaccg
     3781 gcaatgtgaa attttctccc gccgaatccg atcaatcggg cagttgcgtg atcgaagcga
     3841 agacaatcgc caagacttat ggggccgggc ccgtggtgcg ggatttctcc atccggattc
     3901 tgcgcgggga ccgcgttgga attgttgggc caaatggtgc gggcaagacc acgcttctca
     3961 atcttctgac cggccggctg gaacccgatt ccggcacggt gaagctcggc accaatctca
     4021 gaatcgccac actcgatcaa agccgcgcct tgctcgatcc cgagatgagc ttgagcggcg
     4081 tcttaaccgg cggtgtcggc gacatcgtcc atgccggcgg acaagcgcgg cacgtgatca
     4141 gctacatgaa ggattttctg tttcagcccg aacaggcgcg caccccggtg cacgtgcttt
     4201 ccggcggcga gcgggcgagg cttctgatcg caaaggcgct ggcgacgccg tccaatctcc
     4261 tagtgctgga tgaacccacc aacgatctgg acctcgaaac gctcgatctg ctggaggaaa
     4321 tgatcgccga ttatcggggc accgcggtga tcgtcagcca tgatcgcgat tttctcgacc
     4381 gggtcgccac ttcggtgttg atggccgaag gcgatggccg tttcgtcgaa tatgccgggg
     4441 gttacaccga catgctggcg cagcgcggcg aaggcgtatc ggcgaaggaa ccgcccaaac
     4501 ccgttgactc acgtgccgca gcgaaggagc gcaaccccgc ttcggccaag cgcaaactga
     4561 gcttcgccga aaaacacgcg ctcgacacgc tcccgtcacg catcgcggcg ctcacgggtg
     4621 aaatcgccgc tctgcagtcg caactttccg acagcgccct ttacgcccgc gaccccaagc
     4681 gctttctcga aaccagcgcg aagctcgaaa catcccagaa cgaccttaat gccgccgaaa
     4741 tgcgctggct ggagctggaa atgctgcgcg aggatatcga aggcgcgggg tgactacagc
     4801 gacccgattc cggccccgat gaggaacagg agcgcaccga tcgccgcacc caccagcgtt
     4861 ccattgatcc ggatatattg cagatcgccg ccgacatggg attcgatggt cgccgccact
     4921 ttcggaccgt cccagctctc gacgatctcc gcgatatatt tgccgatctg gattcgccag
     4981 ggcgcgatgg aatcgaccag cacgccctcg atggcggcat tgagataatc ctgcatcgcg
     5041 ggcatctgct gcaacaaccg gccaaaagtc ctcacaatct tcactgtcac aatcgccgtc
     5101 ttgggcgaag cgccggctgt atcggcaatc agttcgcgct tgacgtcgtc ccatatcgtg
     5161 cccaacgcgt tgagcaatgc atgattggag gcgacggcgt tctgaatctc cgcgagcagt
     5221 ttgggataat cggccgagcg gcggaatgcc atgacgctat cgcgcatcca gcaatccagc
     5281 cgcgtccgct cgtcgcttcc cggtgtgacg atcgcattca gccaggcctg ggcgccatcg
     5341 acgatggcct gggcgatgcg ccggtcgaaa tagcgtggga aaaatcgccc ggtgcgttca
     5401 ccgacacgct ccacgatcgg ctccctgagt gcctgcagcg aaggccccgc catggcgacg
     5461 cccgcatcga tgaagtccat gtgcttgccg gttcccacga aatcgtccag cagcccgtcc
     5521 acgatgggca cgaactcgac ccggctgatg ccctgttgcg cgaggttgcg gacaaagtta
     5581 tggacatcgg catcgttcag ccccgagagc agcggcggta ccagggcggc agtgcgcgcg
     5641 gcgatgaaat ccgcggcctc ttcggtgtct agccattgcg cgatctggcc cgcgcgattg
     5701 tgccgtctca acctctccgt cagaagggcg gggtccagaa aggaatcgcg cacgaattgg
     5761 ccgagcgcgc ggccgatctc gttcttgcgt gacgggatca gcgcggtgtg cggaatgggc
     5821 aggcccagcg ggtggcggaa caaggcggtg atcgcgaacc aatcggccaa gccgccgacg
     5881 atggccgctt cggcggcggt gttgatcagg cgg
//
LOCUS       DASYEG010000095         3041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_29651, whole genome shotgun sequence.
ACCESSION   DASYEG010000095 DASYEG010000000
VERSION     DASYEG010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29651"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2116
                     /locus_tag="VGM72_03640"
     CDS             <1..2116
                     /locus_tag="VGM72_03640"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEY4264385.1"
                     /translation="GQLGVKDIVIGMPHRGRLNMLANVMAKPYRQLFHEFAGGSAKPS
                     DVQGSGDVKYHLGASSDRVFDANEVHLSLTANPSHLEAVDPVVLGKSRAKQFQYGDKV
                     TRASVLPLLMHGDAAFAGQGVVAECFAMSGTKGFRTGGTIHFIVNNQIGFTTTPGYAR
                     SSPYPSDVALMVQAPIFHVNGDDPEAVVHCCRIATEYRQLFHKDVVVDMFCYRRFGHN
                     ESDEPFFTQPLMYRAIKEHPTTLELYSKKLIGEGVVSEKEVADMIAAFQQRLEQDLEV
                     AKSHLPNKADWLDGRWTGLETAPRGDRRGTTEVDMETLKKVGMAISHVPENFHAHRTI
                     ARLMQTKRQMIETGEDIDWATGEALAFGTLIDDGFPVRLSGQDSSRGTFSQRHAALID
                     QETEERYIPLNHIREDQPEFEVVDSLLSEEAVVGFEYGYSLAEPKALVLWEAQFGDFA
                     NGAQVVIDQFIASGEMKWLRMSGLVMLLPHGFEGQGPEHSSARMERYLQLCADDNMQV
                     AYCTTPANYFHVLRRQLHRNFRKPLILMTPKSLLRHKRAVSKLSEFGPGSSFHRVLWD
                     DAETAPGSTITLVPNEEIRRVVLCTGKVYYDLLEEREKRNETRIQLLRIEQLYPFPGL
                     ALTDELTRFPNAEIIWCQEEPKNQGAWTFLAPNIEGVMEQLGRTGPLRYTGRKASASP
                     AAGQMSQHLAELKAFLNDALTL"
     gene            2211..>3041
                     /locus_tag="VGM72_03645"
     CDS             2211..>3041
                     /locus_tag="VGM72_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEY4264386.1"
                     /translation="MSIEIKVPAMGESVTEATIARWLKKEGDSVNRDEPLVELETDKV
                     NVEVPSPAAGVLEHISIQQGGTVGVGTVLGAVAEGAAAAKPSAQPESAKPAKAKTPEA
                     KSAPAPAAAAAPPPPPPPSVRRIAEEEKLDLSTVAGSGKRGQITKADALAALEAVKTP
                     MSAPPVRASAPSPRGDREERVPMSRLRKTIALRLKESQDIAAQLTTFNEVDMSKLMAL
                     RMAYKDGFEKKHGVKLGFMGFFVKACIAALKEIPAVNAEIDGDDIVYKNYFDIGVAVS
                     T"
BASE COUNT          631 a          918 c          923 g          569 t
ORIGIN      
        1 cggccaactg ggcgtcaagg acatcgttat cggcatgccg catcgcgggc ggctgaacat
       61 gctcgccaat gtgatggcca agccctatcg tcagttgttc cacgaattcg ccggcggttc
      121 tgcgaaaccc tctgacgtgc agggctcggg cgacgtcaaa tatcacctcg gcgcttcttc
      181 cgaccgtgtg ttcgacgcta atgaggtgca tctgtccctc accgcgaacc cctcgcatct
      241 tgaagcggtg gacccggtgg tgctcggcaa atcgcgcgcc aagcaattcc aatatggcga
      301 caaggttacc cgtgcgagcg tcttgccgct gctgatgcac ggcgatgccg ccttcgccgg
      361 ccagggcgtc gtggcggaat gtttcgcgat gtcgggaacc aagggattcc gcaccggcgg
      421 cacgatccat ttcatcgtca acaatcagat tggtttcacc acgacacccg gctatgcgcg
      481 gtcatcgcct tatccttcgg atgtcgcgct gatggtgcag gcgccgatct tccacgtgaa
      541 tggggatgat cccgaagcgg tggtgcattg ctgccgtatc gcaaccgaat accggcagct
      601 gttccacaag gacgtggtgg tggatatgtt ctgctatcgc cgcttcggcc acaacgaaag
      661 cgacgagccg ttcttcacgc agcccttgat gtatcgcgca atcaaggagc atcccaccac
      721 gctggaattg tattcgaaga agctgatcgg tgaaggtgtg gtcagcgaaa aagaagtcgc
      781 cgacatgatt gccgccttcc agcaaaggct cgaacaagat ctggaagtcg ccaaatccca
      841 tctgccgaac aaagccgatt ggctggacgg tcgctggacg ggcctggaaa cggcaccgcg
      901 cggcgatcgc cgcggtacca ccgaagtcga tatggaaacc ttgaagaagg tcggcatggc
      961 gatttcgcat gtgccggaga attttcatgc ccaccgtacc atcgcgcgtt tgatgcagac
     1021 caagcgtcag atgatcgaaa ccggcgaaga tatcgactgg gcgaccggcg aggcgctggc
     1081 attcggcact ctgatcgatg atggattccc ggtgcgtctg tcgggccagg attcgtcgcg
     1141 cggcaccttc tcgcagcgcc acgcggccct gatcgatcag gagaccgaag agcgctacat
     1201 cccgctgaac catatccgcg aagatcagcc ggaattcgaa gtggtcgatt cgctgctttc
     1261 ggaagaggcg gtggtcgggt tcgaatatgg ctacagcctc gccgagccca aggcgctcgt
     1321 gctttgggaa gcgcaattcg gcgatttcgc caatggcgcc caagtggtga tcgatcagtt
     1381 catcgcctcg ggcgaaatga aatggttgcg catgtcgggc cttgtcatgc tcttgccgca
     1441 cggcttcgaa ggtcagggac ccgaacattc ctcggcccgc atggagcgct atctgcagct
     1501 ctgcgccgac gacaatatgc aggtggccta ttgcacgacg ccggccaatt acttccacgt
     1561 gttgcgccgc caattgcatc gcaatttccg caagccgctg atcctgatga cgcccaaatc
     1621 gctgctgcgc cataagcggg cggtatccaa gctgtccgag ttcggaccgg gttcgtcctt
     1681 ccatcgcgtg ctgtgggacg atgccgagac cgcacccggt tccacgatca cactggttcc
     1741 caacgaggaa atccggcgcg tggtgctctg caccggcaag gtctattacg atcttctgga
     1801 agagcgcgag aagcgcaacg aaacccgcat ccagcttttg cggatcgagc agctttatcc
     1861 cttccccggc ctggctctga ccgatgaact gacgcgcttt cccaatgcgg agatcatctg
     1921 gtgccaggag gagccgaaga atcagggcgc ctggacgttc cttgcgccga acatcgaagg
     1981 cgtcatggaa caattgggac gcaccggtcc gttgcgctat accgggcgta aggcatcggc
     2041 gtcaccggcg gcaggtcaga tgagtcaaca tcttgccgaa ctcaaagcct tcctcaacga
     2101 cgctctgacg ctgtaatgga tgcagactcg cccctcgaca gctcggggtg aggaaaaggt
     2161 ttggtcctca tgctgagctg atcggcgcat gaggtgcagg ggtttcgcat atgagcattg
     2221 aaatcaaagt accggcgatg ggcgaatccg tcaccgaggc gacgattgcc cggtggctga
     2281 agaaagaagg cgattcggtc aaccgcgacg aaccgttggt cgaactcgaa accgacaagg
     2341 tcaatgtcga ggttccttca ccggcggccg gcgtgctcga acatatcagc atccagcagg
     2401 gcggcacggt gggcgtcggc acggttctgg gcgcggtcgc ggaaggtgcg gcggctgcca
     2461 aaccatcggc ccaaccggaa agcgccaaac ccgcaaaagc gaaaacgccg gaagccaagt
     2521 ctgcgccagc acccgccgcg gctgccgcac cccctccgcc accaccgcct tcggttcggc
     2581 gcatcgccga ggaggagaag ctcgaccttt cgactgtcgc cggatccggc aagcgcggcc
     2641 agatcaccaa ggctgacgcg ttggcggcgc tggaagcggt gaaaaccccg atgagcgcgc
     2701 cgccggtccg ggcctcagcc ccatcgccgc ggggcgaccg cgaagaacgc gtgccgatgt
     2761 cgcggctgcg caagaccatc gcgctgcgtc tgaaggaaag ccaggacatc gccgcccagc
     2821 tcaccacgtt caacgaagtc gatatgtcga agttgatggc ccttcgcatg gcctataagg
     2881 acggcttcga aaagaagcac ggcgtgaagc tcggcttcat gggcttcttc gtgaaggcct
     2941 gcatcgccgc cttgaaagag attcccgcgg tgaacgccga aatcgacggt gacgacatcg
     3001 tctacaagaa ttatttcgac atcggcgtgg cggtttcgac c
//
LOCUS       DASYEG010000096         2989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_901119, whole genome shotgun sequence.
ACCESSION   DASYEG010000096 DASYEG010000000
VERSION     DASYEG010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901119"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VGM72_03650"
     CDS             <1..545
                     /locus_tag="VGM72_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY4264387.1"
                     /translation="RKLVRLKGGDPAIFGRLGEEIAALQHACIPFAIVPGITAASAAA
                     AASGISLSLRGRARRVQFVAAHARHGEELELDWPALADNSATTVFYMARESASLIAER
                     LISHGLSASTPVLLMSDVSHTTEMRMCVALSGLADAVENFPPAVPLIILIGEVTEGAA
                     THAHRVGAFSQALALHPACA"
     gene            555..>2989
                     /gene="nirB"
                     /locus_tag="VGM72_03655"
     CDS             555..>2989
                     /gene="nirB"
                     /locus_tag="VGM72_03655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HEY4264388.1"
                     /translation="MNMIAPKREHLVVVGNGMAGMRTVEELLKRPGGKHFRITVFGAE
                     PHVNYNRIMLSSVLAGDKSVDDIIINSREWYDENGITLIIGDPVKRIDRQARTITSAN
                     GVTVGYDNLLIATGSRPLAPPIPGLSLPGVCAFRDIADVDKMLEAARTHKRAVVIGGG
                     LLGLEAAWGLKQRGMSVALVHLMPTLMERQLDAASGQLLQRDLDRRGIAFFTDGQTEE
                     ITGSERAEGVRLADGRFIPADLVVLAIGIRPNIDLARAAELEVNRGIVVADDMRTSDS
                     DIFAVGECVEHRGQVFGLVAPLWDQAKVCAAQLAGDETALFSAGALSTSLKITGVDVF
                     SAGALMAADESDDEITLRDDNRGLYKKIVLREGRLVGAVMYGDVADGQWYLRLIREKT
                     DVAGLRDRLVFGRAFAEANKPPSADSDVVGMADEAQICGCNGVSKSTIVCAIKGKELT
                     SLSQVRAHTKASASCGQCTPLVEALLAHTTGVVAEKKIAPLCDCTQAGHDEIRRAICE
                     QNLKTMQAVRDAFGWAKKEGCHKCRPALNYYLICAWPGEYRDDSRSRFVNERVHANIQ
                     KDGTYSVVPRMWGGVTTPAELHAIASVAEKFAIPTVKITGGQRIDLLGVKKEDLPAVW
                     GDLNKAGLVSGHAYAKGLRTVKTCVGTEWCRFGTQDSTGLGIKLEKMCWGSWTPHKVK
                     FAVSGCPRNCAETTIKDMGVVCVEAGYDILVGGNGGLEVRVTDPLVRVASEAEVLEYA
                     GAFMQLYREEAHYLERTAPWIARVGIDYVKNRLVEDGDNRRALHARFLHSQTFVQTDP
                     WADRAKGADAHEFA"
BASE COUNT          586 a          867 c          943 g          593 t
ORIGIN      
        1 gccgaaaact cgtgcgcctc aagggtggag acccggcaat tttcggacgc cttggcgagg
       61 aaatcgccgc gctccagcat gcttgcattc cattcgcaat tgtaccgggc atcacggcgg
      121 catctgcagc ggccgcggca agcggaatct ctctctccct gcggggccgc gcgagacgcg
      181 tgcaattcgt ggcggcccat gcgcgtcacg gcgaagaact cgaactcgat tggcccgccc
      241 tcgccgacaa ttccgcgacc accgttttct atatggcgcg cgaatccgca agcctgattg
      301 ccgagcggtt gatatcccat gggctcagcg caagcacgcc ggtgctgttg atgagcgatg
      361 tcagccatac caccgaaatg cggatgtgcg ttgccctgtc gggtttggcg gatgcggtcg
      421 aaaattttcc gccggctgtg cctctgatta ttctcatcgg cgaagtgacg gaaggagccg
      481 ccacccacgc acatcgggtt ggagcatttt cccaagccct cgcgctccac cctgcctgtg
      541 cgtaggagcc atccatgaac atgatcgcac ccaagcgcga gcatctggtt gtcgtgggca
      601 atggcatggc cggaatgcgc accgtcgagg aattgctgaa acgccccggc ggcaaacatt
      661 ttcgcataac ggttttcggg gcggagccgc acgtcaatta caaccgcatc atgttgtctt
      721 cggtgctggc cggcgataag agcgtcgatg acatcatcat aaattcgcgc gaatggtatg
      781 atgaaaacgg catcacgctg atcatcggtg atccggtcaa acgcatcgac cggcaggcgc
      841 gcactattac atccgcaaat ggcgtcacgg ttggttacga taatctactg atagccacgg
      901 gctcacgtcc gctcgccccc ccaatcccgg ggctctccct tccgggtgtt tgcgcattcc
      961 gtgacattgc cgatgtcgac aagatgctgg aggccgcacg cacccataag cgtgccgtgg
     1021 tgatcggcgg cgggctgctg gggctggagg cagcctgggg cctgaaacag cgcggcatgt
     1081 cggtggcgct cgttcatctg atgccgactc taatggagcg tcagttggac gccgcttccg
     1141 gtcagcttct gcaacgcgat cttgaccgcc ggggcattgc gtttttcacc gatggtcaga
     1201 ccgaggagat caccggcagt gaacgcgccg aaggtgtgcg gcttgcggac ggacggttca
     1261 ttccggccga tctggtggtc ctggccatcg gcattcgtcc caatatcgat ctggcccgcg
     1321 cggcagagct ggaggtcaat cgcggaatcg tggtcgcgga cgatatgcgc accagcgatt
     1381 ccgatatttt tgccgtcggc gaatgcgtgg agcatcgcgg tcaggtcttt ggtctggttg
     1441 ctccgctttg ggatcaagca aaagtctgcg ccgcgcagct cgccggagat gaaacggctc
     1501 tgttctccgc cggggctttg tccaccagcc tcaaaataac cggtgtcgat gtgttctcag
     1561 ccggggccct gatggccgcg gacgaaagcg acgatgagat cacgctgcgc gacgacaatc
     1621 gcgggctgta caaaaaaatt gtgctgcgcg aaggccggct ggtgggtgcg gtgatgtacg
     1681 gcgatgtcgc ggacggtcaa tggtatttgc gcctgatacg ggagaagacc gatgtggccg
     1741 ggctgcgcga ccggttggtt ttcggccgcg catttgccga agcaaacaaa ccgccatctg
     1801 cggattcgga tgtcgtgggt atggccgacg aggcacagat ctgcggctgc aacggcgtgt
     1861 ccaaatccac catcgtttgc gccatcaagg ggaaagaact tacgtcactc tcgcaagtgc
     1921 gagcccacac caaagcgtcg gcgtcttgcg gccagtgcac gccactggtc gaagcgcttt
     1981 tagcgcacac aacgggcgtg gtggccgaga agaaaatcgc ccccctctgc gattgcacgc
     2041 aggcgggaca tgacgaaatc cgccgggcga tttgcgagca gaatctcaaa accatgcagg
     2101 cggtgcgcga tgcgttcggc tgggcgaaga aagagggttg ccacaaatgc cgtccggcgc
     2161 tgaattatta tctaatttgc gcctggccgg gcgaatatcg cgacgattcc cgttcgcgct
     2221 tcgtcaatga gcgcgtacac gccaatatcc agaaagacgg cacttattcc gtggtgccgc
     2281 ggatgtgggg cggagtcaca accccggcgg aattgcacgc catcgcatcg gtcgcggaaa
     2341 aatttgccat tcccaccgtg aagatcacag gcgggcagcg catcgacctt ctcggcgtca
     2401 agaaagaaga tctgcctgcg gtgtggggcg atttgaacaa agccggcctg gtctccggcc
     2461 acgcctatgc caaaggcctt cgcaccgtta agacctgcgt tggcaccgaa tggtgccgct
     2521 tcggtacgca ggattcgacg ggcctcggta tcaagctcga aaaaatgtgc tggggctcct
     2581 ggacgccgca caaggtgaaa ttcgcggtat cggggtgtcc gcgcaattgc gcggaaacca
     2641 ccatcaagga tatgggcgtg gtgtgcgtcg aggcgggata cgacattctg gttggcggca
     2701 atggcgggct tgaggtgcgt gtcaccgatc cgttggttag ggtcgcgagc gaagccgaag
     2761 tgcttgaata tgcgggcgcc ttcatgcagc tctaccgtga ggaggcgcat tatctcgaac
     2821 gcaccgcacc ctggatcgcg cgcgtcggga tcgattatgt gaagaatcgc cttgtcgagg
     2881 acggcgacaa tcgccgcgct ctgcacgcgc ggtttctcca ttcccagaca ttcgtgcaga
     2941 ccgatccttg ggcggatcgc gcgaagggtg ccgatgccca cgagttcgc
//
LOCUS       DASYEG010000097         8630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3654681, whole genome shotgun sequence.
ACCESSION   DASYEG010000097 DASYEG010000000
VERSION     DASYEG010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 8630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8630
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3654681"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="VGM72_03660"
     CDS             complement(<1..1245)
                     /locus_tag="VGM72_03660"
                     /inference="COORDINATES: protein motif:HMM:NF011999.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HEY4264389.1"
                     /translation="MSNLDVFDLFNRDYSREQLETMSLRDWLLAARDDKMLYASPAER
                     MISAIGEQELIDTAQDPRLGRIFFNRTIKRYKAFDGFFGMEDTIERIVGYFKHAAQGL
                     EEKRQILYLLGPVGGGKSSLAERLKDLMEKQPIYVLKAGDQISPVFESPLGLFRSADL
                     AALLEDKYGIARHRLTSVMSPWAVKRLDEFNGDISKFEVARLYPSKLRQIAVAKTEPG
                     DENNQDISSLVGKVDIRKLEHFSQNDPDAYSFSGGLCHANQGLLEFVEMFKAPIKVLH
                     PLLTATQEGNYIGTETLGPIPFNGVILAHSNEAEWQVFKANKNNEAFLDRICVITVPY
                     CLRVTEEVQIYKKLLKESQLDKASCAPETLEMLGRFCVLTRLKEHENSNLYSKMRVYD
                     GEKLRDTDPKAKTLQEYKDMAGV"
     gene            1735..2277
                     /locus_tag="VGM72_03665"
     CDS             1735..2277
                     /locus_tag="VGM72_03665"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4264390.1"
                     /translation="MGTKLIIRKARPTDAEGVARVYVASWRDTYPLVLPTRLLSSMTI
                     EGQSARWRNAITIAAREAVYVAEDEAGRIAGMTSMGRARDSGLGYDAEIYTLYVDPML
                     TGTGIGRALLAGAFEALAERGHARCVIWAHAGNPARFFYHAMGGRLIAERTTAMMGAP
                     VPEVAFGWTKLALAEASRSN"
     gene            complement(2274..3101)
                     /locus_tag="VGM72_03670"
     CDS             complement(2274..3101)
                     /locus_tag="VGM72_03670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4264391.1"
                     /translation="MSTSREGAGRTALITGASAGIGMALARVFAANGFDLVLTARRAE
                     RLNALAAELRAAHGIAAHVFLADLALPETPARLIDAIQQAGLTIDALINNAGYGVAGL
                     YRETPWEAQRDFLQVLVTAPCELTHRLLPAMTNRGYGRILNVASVAGFMPGAARHTLY
                     GAAKALMIKFSQSLHSEQSQTGVHVSALCPGFTHSEFHDVNGTRSAMSRLPEFMWLSA
                     ERVAEDGYRAVMKNDPVCVPGAQYKFITAMVRLLPMKMAYRLTQRRSPMAGSGARES"
     gene            complement(3098..3691)
                     /locus_tag="VGM72_03675"
     CDS             complement(3098..3691)
                     /locus_tag="VGM72_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY4264392.1"
                     /translation="MSARRALPSVEALFERRSLRLLLLRHAKSDWSAHTGDHERPLSA
                     RGRKAVPRMASRMRREGYRPAYVLCSTAQRAKETAALLMPLLEFTDGIRYDRALYLAE
                     WPQLLAEIHSVVQDMSPLLLVGHNPGMEQLAMALALQPQSPGERARAESLADKFPTAA
                     LAVLDFAMAQWSAIKPGSGRLVDYIRPKDLGAGGGDE"
     gene            3880..5082
                     /gene="metZ"
                     /locus_tag="VGM72_03680"
     CDS             3880..5082
                     /gene="metZ"
                     /locus_tag="VGM72_03680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEY4264393.1"
                     /translation="MATNKDWANWKRQTLAVRGGQRRSPFQETSEALYLTSGYAYEGP
                     EEAEERFKGAPGYTYTRYGNPTISIFEERMALLEGAPVGRATASGMAAVTATFLCCLK
                     AGDHVVAARAMFGAIRYVVEDVLARFGITSTIVDGTDLESWRAAMRPETKIVFFETPS
                     NPTLEIVDIAGVADIAHKFGARVVVDNAFASPALQRPMEFGADIVVHSSTKYIDGQGR
                     AMGGIILCKEDFLKDHLQIFLRNTGPALSPFNAWVHLKSLETLDLRMREHCRNAVAIA
                     DFLAAQKNVRLVIYPFRESSRTNAAQIALARKQMSGGGGVVTFEVAGGKKEAFKVAKS
                     LKLIDVSNNLGDTKSLLTHPATTTHQRLTLEARLAAGVTDGMLRISVGLEDPADVCDD
                     LAQALSKI"
     gene            5094..5540
                     /locus_tag="VGM72_03685"
     CDS             5094..5540
                     /locus_tag="VGM72_03685"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY4264394.1"
                     /translation="MEHHATIDWRRTTDTYTYETYNRAHEWHFHAVTVAASSAPDFRG
                     ERERVNPEEAFIAALSSCHMLTFLAIAAKKKLPLDSYRDEAIGYLEKNAAGRLAITRV
                     ILRPRIQWPQGVTVAQADLETMHHQAHEGCFIANSVNTNVTVEPQH"
     gene            5537..5941
                     /gene="apaG"
                     /locus_tag="VGM72_03690"
     CDS             5537..5941
                     /gene="apaG"
                     /locus_tag="VGM72_03690"
                     /inference="COORDINATES: protein motif:HMM:NF003967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEY4264395.1"
                     /translation="MSRHRYEETTRDIRIAIEPDFLEDQSEPEENRFLWSYRVVIENK
                     SALAVQLLSRYWRITDARGRVREVRGDGVVGEQPVIAPGRAFEYTSGAPLETASGFMT
                     GTYHMRASTGESFEVGIPMFALESPYEAHRMH"
     gene            6009..6641
                     /gene="folE"
                     /locus_tag="VGM72_03695"
     CDS             6009..6641
                     /gene="folE"
                     /locus_tag="VGM72_03695"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEY4264396.1"
                     /translation="MDTCRLSLITPSTPTEPKPSREEAEAAVRTLLRWAGDDPEREGL
                     RDTPARVARAYEDWFSGYADDPVSFLQRTFEEVEGYDEMVVLRDIRFESHCEHHLAPI
                     IGRAHVGYLPDRKVVGISKLARVVDAYARRLQVQEKMSAQIANCIQHILEPKGVAVVI
                     EAAHQCMTTRGVHKTGVTMVTSMMLGAFREDSRTRREFLAMIHHPVAPGS"
     gene            6652..7803
                     /gene="argE"
                     /locus_tag="VGM72_03700"
     CDS             6652..7803
                     /gene="argE"
                     /locus_tag="VGM72_03700"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF005710.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HEY4264397.1"
                     /translation="MLSGTPIVDMLGRLIAFDTTSRNSNLDLIAFARETLEKSGATIR
                     LTYDSDKRKANLFATLGPERDGGYVLSGHSDVVPVDGQEWSSNPFKSEIRDGRLYGRG
                     AADMKGFIAAALALAPEFAAADLKRPIHIALSFDEEVGCVGVRRLLADLESAGIRPAL
                     ALIGEPTEMRVVAAHKAGAVIETIVHGREGHSSAPALGANAVMMAGEFIAALSAIGND
                     LMAERDEFFEPPYTTVQANMISGGTAVNVLARDAKVIWEYRALPDRDTQTILERAQAA
                     AQAILPRYREGAPNADFQTRIKAAYPGLKRDSHSPAVHLASALCGSNDVHAVSYGTEA
                     GLFQQAGIPAAICGPGSITQAHRADEFVAVDQLDACAAFLRRLLQRAAE"
     gene            complement(7854..>8630)
                     /locus_tag="VGM72_03705"
     CDS             complement(7854..>8630)
                     /locus_tag="VGM72_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEY4264398.1"
                     /translation="PEAGQRVLAEALALSALLGSSLKFEGRLSVQTKGDGPLNLLATD
                     YFADGGLRGYARVDEKRFAEAGRPNHFGSLVGKGALAITIEPKKGAQAYQGLVPLSPD
                     GLAASAETYFAQSEQLATRLRLAAGPIYRADAGHGWRAGALMVQAVPGARIADVTASD
                     DWRRVSLFLQTLEDVELLDTAVAAESILWRLFHEDEVRVHPSQKLRFQCSCESERVRT
                     VLKSYSAEALKDLPDPDGVIRTRCEFCGSVYEFPLESLAR"
BASE COUNT         1663 a         2700 c         2640 g         1627 t
ORIGIN      
        1 gacgcccgcc atatccttgt attcctgcaa ggtcttggcc ttgggatcgg tgtcgcgcag
       61 tttttcgccg tcatagacgc gcatcttcga ataaagattc gagttctcgt gctccttgag
      121 ccgcgtcagc acgcagaagc gccccagcat ttccagcgtc tcgggcgcgc aggaggcctt
      181 gtcgagctgg ctctccttca agagcttctt gtagatctgc acttcctcgg tgacgcgcag
      241 gcagtacggc acggtgatga cgcagatgcg gtcgaggaaa gcttcgttgt tcttgttggc
      301 cttgaagacc tgccattcgg cctcgttcga atgggcgagg atgacgccgt tgaacggaat
      361 gggaccgagc gtttcggtgc cgatgtaatt gccttcctgg gtggcagtca gcagcgggtg
      421 caagaccttg atcggcgcct tgaacatctc gacgaattcg agaaggccct gattggcgtg
      481 gcagagcccg cccgagaagg aataggcgtc cggatcgttc tggctgaaat gttcgagctt
      541 gcggatatcc accttgccga cgagcgagga aatatcctgg ttgttctcat cgccgggctc
      601 ggtcttggcg accgcgatct gacgcagctt cgaaggataa agccgcgcca cttcgaattt
      661 ggagatgtcg ccgttgaact cgtccagccg cttcaccgcc cacgggctca tcacgctggt
      721 gagccggtgg cgcgcgatgc cgtatttgtc ttcgagcagt gccgccagat cggcggaacg
      781 aaacagcccg agcggacttt cgaacaccgg actgatctgg tctcccgcct tcaacacata
      841 gatcggctgc ttctccatca aatctttgag acgctcggcg agcgaggatt taccgccgcc
      901 gaccggtccc agcagataga gaatctgccg cttctcttcc aatccttgcg ccgcgtgctt
      961 gaaatagccc acgatacgtt cgatcgtgtc ttccatgccg aagaaaccgt cgaacgcttt
     1021 gtagcgcttg atggtccggt tgaagaagat gcgccctagc cggggatctt gcgccgtgtc
     1081 gatcagctcc tgctcgccga tcgcggagat catgcgttcc gccggagagg cgtacagcat
     1141 cttgtcgtca cgcgcggcga gcagccaatc gcgcagactc atcgtctcca attgttcacg
     1201 cgaatagtcg cggttgaaca ggtcgaatac gtcgaggtta ctcattctct cccgctcctt
     1261 ccggtcgggc taagcccagg tgaacaaaac aagatgtcga atacatggcg aagctcgcgc
     1321 caaatcaatg cacgatctgt gcctcgttgc atgaacgatt gcttaacttt gtcttttctt
     1381 tgaggttgag tgcgtccggt ttcgcaagcc ttgcccgcga aacgcgcaac tccacatgac
     1441 gcggcaatcg ccaaatgaac cccgcgaact gcggccttcc gtcttgcgaa tgcggccgcc
     1501 gattcccaat tgtaccgcga gacaggaaga tgcagaaggg aggctgggca atcatgctgt
     1561 gtaaaaacac aagcagcatt catgcatgct cttctgattc acgaggaaaa tcgctccacg
     1621 gagcgcactt gcgtcaataa cgcacagagg caatcaccgt tccgctcaag gcttgcggtt
     1681 agattcttgc ggatttttct tgaacccgcg ttcgcgtacg gtcaagctcg aacgatgggc
     1741 acgaagttga tcatccgcaa agcacgcccg accgatgccg agggcgtggc gcgagtttat
     1801 gtcgcttcct ggcgcgatac ctacccgctg gtgctcccga cccgcctgct gtccagcatg
     1861 accatcgaag gccagagcgc gcgctggcga aatgccatta ccatcgccgc acgcgaagcg
     1921 gtctatgtcg ccgaggatga agcgggccgg atcgccggca tgacgagcat gggccgcgcg
     1981 cgggattccg gccttggata tgatgcggaa atttatacgc tctatgtcga tcccatgctg
     2041 accgggaccg ggatcggccg ggcgctgctg gccggggcct tcgaagcgct ggccgaacgc
     2101 gggcacgcac gttgcgtgat ttgggcccat gccggaaacc cggcacgatt cttctatcac
     2161 gcgatgggcg ggcggctgat cgcagagcgc accaccgcga tgatgggagc gccggttccg
     2221 gaagtcgcct tcgggtggac gaagcttgcc ctggccgagg ccagccgcag caattaggat
     2281 tcgcgtgcgc ctgagccggc catgggcgag cgccgctggg tgaggcgata cgccattttc
     2341 atcggcagca atctcaccat cgcggtaatg aatttatatt gcgcgcccgg aacgcagacc
     2401 ggatcgtttt tcattacggc acgatagcca tcctcggcca cacgctcggc ggacagccac
     2461 atgaattccg gcaagcggct catcgcggac cgcgttccat tgacgtcgtg gaattcgctg
     2521 tgcgtgaacc ccggacacag cgcgctcacg tggacgccgg tttgcgattg ctcgctgtgc
     2581 agggattgcg aaaatttgat catcagcgct ttggcggcgc catacagcgt atgacgcgcc
     2641 gcgcccggca tgaatccggc aaccgaggcg acattcaata tccggccgta gcccctgttc
     2701 gtcatggcgg gaagaaggcg gtgtgtgagc tcgcaaggcg cggtcacaag cacctgaaga
     2761 aaatcgcgct gcgcctccca cggcgtctcg cggtaaagtc cggcaacccc gtagcccgca
     2821 ttgttgatca gcgcgtcgat cgtgaggccc gcctgttgaa ttgcgtcaat cagcctggcg
     2881 ggtgtttcgg gaagcgccag atcggccagg aacacatgtg cggcaattcc atgggccgcg
     2941 cgcaattcgg cggcgagcgc attcagccgt tcggcacgcc tggccgtcag caccagatcg
     3001 aaaccattgg cggcaaagac ccgggccagc gccatcccga tcccggccga tgcgcccgtg
     3061 atcagcgcgg tgcgtcccgc cccttcacgc gatgtgctca ttcgtccccg ccgcccgcgc
     3121 caagatcctt gggacggatg taatcgacga gccgtcccga tccgggtttg atcgcgctcc
     3181 actgcgccat cgcaaaatcc agcaccgcga gcgcggcggt cggaaattta tcggcgaggc
     3241 tttcggctcg cgcacgttcg ccggggcttt gcggctgcag cgcgagcgcc atcgccaatt
     3301 gctccatgcc tggattatgc ccgaccagaa gtaacggcga catgtcctga accacgctat
     3361 ggatttcagc cagaagctgc ggccattccg ccagatacag cgcgcgatcg taccgaatgc
     3421 cgtccgtgaa ctccaaaagc ggcatcagaa gtgccgcggt ttctttggcg cgctgagcgg
     3481 tggaacaaag cacataagcg ggccgatacc cctctctacg catccgcgac gccatgcgcg
     3541 gcacggcctt tcgtccgcgc gcggaaagag gccgctcgtg gtcgccggtg tgggccgacc
     3601 aatccgattt ggcgtgtcgc aggagcaata gccgcaatga acgcctctcg aatagagcct
     3661 caacactagg cagcgcccgg cgtgctgaca agcgatgcct ggcttttcaa tcgccccacg
     3721 gatcgtgcga accggcaaga aaagtccccg cgccatccgc cgctggattt tttgcacgct
     3781 ctcgttgcgg ttgtcagagg gagcgccggg accgctcacg cttgacgtgt ggacaggcgg
     3841 accctatcgc tttgctttca cacggcctgc ggatttgaca tggcgacgaa taaggattgg
     3901 gcgaattgga aacggcagac gctcgcggtc cgcgggggcc agcggcgtag cccgtttcag
     3961 gaaacgagcg aggctctgta cctcacttcg ggctacgcct atgaggggcc ggaagaagcc
     4021 gaggaacgtt tcaagggcgc gcccggctac acctatacgc gctatggcaa tccgacgatt
     4081 tcgattttcg aagaacgcat ggcgctgctg gaaggcgcgc ctgtcggacg tgccacagcg
     4141 tcgggcatgg cggcggtcac cgcgactttt ctgtgctgcc tgaaggcggg cgatcatgtg
     4201 gtggccgcgc gtgcgatgtt cggcgcgatc cgctatgtgg tcgaggacgt cctggcgcgc
     4261 ttcggcatca ccagcaccat cgtggacggc accgaccttg aatcctggcg ggcggccatg
     4321 cgcccagaga cgaaaatcgt tttcttcgag acgccttcca atccgacgct cgagatcgtc
     4381 gatatcgcgg gcgtggcgga catcgcgcac aaatttggcg cgcgcgtcgt ggtggacaac
     4441 gcgtttgcct cgcccgcgct gcagcgcccg atggagttcg gtgccgatat cgtcgtgcat
     4501 tcctccacca aatatatcga cggccagggc cgcgcgatgg gcggcatcat tttgtgcaag
     4561 gaagatttcc tgaaagacca ccttcaaatc ttcctgcgca acaccgggcc ggcgctgagc
     4621 cccttcaatg cctgggtgca cctgaaaagc ctcgaaacgc tggacctgcg catgcgcgaa
     4681 cattgccgca atgccgttgc cattgcggat ttcctggcgg cgcagaaaaa tgtgcgcctg
     4741 gtgatttatc cgttccgcga aagctcgcga accaatgcgg cgcaaatcgc tcttgccagg
     4801 aaacagatgt cgggcggcgg cggcgtggtg accttcgaag tggcgggcgg aaagaaagaa
     4861 gcgttcaagg tcgccaagag cctgaagctg atcgatgtct ccaacaattt gggagacacc
     4921 aagagcctgc tcacccatcc cgccacgaca acccatcagc gtctcacgct cgaagcgcgg
     4981 ctcgccgccg gagtcaccga cggcatgttg cgcatctcgg ttggtttgga agaccccgcc
     5041 gatgtctgcg atgacctcgc ccaggcgctg tcgaaaatct gaaggaccgt ccaatggaac
     5101 atcacgccac gatcgattgg cgccgcacga ccgacaccta tacgtacgaa acctacaatc
     5161 gcgcgcatga atggcatttc catgccgtga cggtggcggc ctcttcggcg ccggatttcc
     5221 gcggcgagcg tgagcgcgtc aatccggagg aagcattcat cgcggcactt tcctcctgtc
     5281 atatgctgac gtttctcgcg atcgcggcaa agaaaaagct gccgctcgat tcttatcgtg
     5341 acgaggcgat cggctatctg gaaaagaacg cggccgggcg gcttgcgatc acgcgcgtga
     5401 tcctgcggcc gcgcatccaa tggccgcagg gcgtgacggt ggcgcaagcg gacctggaaa
     5461 cgatgcacca tcaagcccat gaaggctgct ttatcgcgaa ttccgtgaac acgaatgtca
     5521 ctgtggagcc gcagcattga gccggcatcg ttacgaagag accacacgcg acatccggat
     5581 cgcgatcgag cccgatttcc tcgaagacca atcggaaccg gaggagaacc gctttctctg
     5641 gtcgtatcgc gtcgtcatcg aaaacaagag cgcgctcgcc gtgcaattgc tctcgcgcta
     5701 ctggcggatc accgatgcgc ggggccgcgt tcgggaagtg cgcggtgacg gcgtggtggg
     5761 cgagcagccg gtgatcgcgc ccggccgcgc cttcgaatat accagcggcg caccgttgga
     5821 gacggcttcc ggattcatga ccggcaccta tcacatgcgc gcctccaccg gcgaaagctt
     5881 cgaggtcggc atcccgatgt tcgcgctcga aagcccttac gaagcccacc ggatgcattg
     5941 atggagccgc gtcctggttt cctgcaatgt ttccactaga atgccgtcat cgctattgga
     6001 gccttcgcat ggatacgtgc cgcctctccc tcatcactcc gtctacgccg acggaaccca
     6061 agcccagccg ggaggaagcc gaggccgccg tgcgcacatt gcttcgctgg gcgggggacg
     6121 atcccgagcg tgagggtctg cgcgatacgc cggcccgcgt tgcgcgcgcc tatgaggatt
     6181 ggttttccgg ctacgcagac gatccggtct catttctcca gcgaaccttc gaggaggtcg
     6241 agggttatga cgaaatggtc gtgctgcgcg acatccggtt cgaatcgcat tgcgagcacc
     6301 atctggcgcc gatcatcggc cgggcccatg tcggctatct tccggaccgc aaggtcgtgg
     6361 gcatctccaa gcttgcccgc gtggtggacg cctatgcccg gcgcctgcag gtccaggaaa
     6421 agatgagcgc gcagatcgcc aattgcatcc agcatatcct ggagcccaag ggcgtcgccg
     6481 tggtgatcga ggccgcgcac caatgcatga cgacgcgtgg cgttcacaaa accggcgtga
     6541 ccatggtgac atccatgatg ctgggcgcgt ttcgcgaaga ttcgcgcacc cggcgcgaat
     6601 tcctcgccat gattcatcat cccgtcgccc cagggtcatg agaaacgctc catgctgtcc
     6661 ggcacaccca ttgtggatat gctcggcagg ctgatcgcct tcgacacgac gagccgaaat
     6721 tccaacctcg atctgatcgc attcgcgcgc gaaacactgg aaaaatccgg cgcgacgatc
     6781 cggctgacct atgattcgga caagcgcaaa gccaatctgt tcgcgacact ggggcccgag
     6841 cgcgacggtg gttacgtgct gtccggccac agcgacgtcg ttccggtaga cggccaggaa
     6901 tggtcgagca atcccttcaa aagcgagatt cgcgacggac gcctctatgg gcgcggcgcc
     6961 gccgacatga aaggcttcat tgcggccgcg ctggcgctcg ctccggaatt cgccgccgcc
     7021 gatctgaagc gccctatcca catcgcattg tcgttcgacg aagaagtggg ctgcgtcggc
     7081 gtgcgccggc tgctggcgga tttggaaagc gccgggatac gccccgcgct cgccctcatc
     7141 ggagagccga ccgagatgcg ggtcgtcgcc gcgcataagg caggggcggt gatcgagacc
     7201 atcgtgcatg gccgcgaagg tcattccagc gccccggcat tgggcgccaa tgccgtgatg
     7261 atggccggcg aattcattgc cgcattgtcg gccatcggaa atgacttgat ggcggagcgc
     7321 gatgaattct tcgagccgcc ctatacgacc gtccaggcca acatgatttc gggcggaacg
     7381 gcggtgaacg tgctcgcgcg cgatgccaag gtgatttggg aatatcgcgc ccttcccgac
     7441 cgtgacacgc agacgatctt ggaacgggcg caagccgccg ctcaggcgat attgccgcgc
     7501 taccgcgaag gcgccccaaa cgccgatttt cagacccgga tcaaggccgc ctatccgggg
     7561 ctcaagcgcg attcccattc gcccgcggtg catctggcat cggcgctttg cggcagcaac
     7621 gacgtgcacg cggtttccta tggcaccgaa gcggggctgt tccagcaagc cgggattccg
     7681 gcggcgattt gcggacccgg ctcgatcacc caggctcacc gggccgatga gttcgtggcg
     7741 gtcgatcagc tcgatgcctg cgccgcgttc ctgcggcggc tcctacaaag agcggcggaa
     7801 tagcatcggc ctatgcccat cgggatagcc cgctgtcgag ggcattagat gtgctaacgc
     7861 gccagcgatt ccagcgggaa ctcgtacacc gaaccgcaga attcgcagcg cgtgcggatg
     7921 acaccgtccg gatcgggcag gtccttcagc gcttcggccg aataggattt cagcaccgtg
     7981 cgcacgcgtt cggattcgca actgcattgg aagcgcagct tctgactcgg atgcacgcgc
     8041 acctcgtctt catggaagag gcgccacaaa atcgattccg ccgcgacggc ggtgtccagc
     8101 aattcaacgt cttccagcgt ctgcaggaaa agcgagacgc gccgccaatc gtcggacgcc
     8161 gtgacgtcgg cgatcctggc acccggcacc gcctgaacca tcagcgcgcc ggcccgccag
     8221 ccatggcccg catccgcgcg atagatggga ccggccgcga gccgcagccg cgtcgcgagc
     8281 tgctcgctct gggcgaaata ggtttcggcc gaagcagcga gtccatcggg cgacagcggc
     8341 accaggcctt gataggcttg cgcccccttt ttgggttcga tggtgatggc aagcgcacct
     8401 ttgcccacca ggcttccgaa atgattcggc cgccccgctt cggcaaagcg cttttcgtcc
     8461 acgcgcgcat atccgcgcaa gccgccgtcc gcgaaataat cggtcgccag gagattgagc
     8521 ggcccatcgc ccttggtctg cacgctgaga cgcccctcga atttcagcga cgaacccagc
     8581 aacgccgaca aggccagggc ctcggccagc acgcgctgac cggcctccgg
//
LOCUS       DASYEG010000098        12061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3798920, whole genome shotgun sequence.
ACCESSION   DASYEG010000098 DASYEG010000000
VERSION     DASYEG010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 12061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12061
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3798920"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="VGM72_03710"
     CDS             <1..1113
                     /locus_tag="VGM72_03710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4264399.1"
                     /translation="PRAATGHSGIITGALPMPILLVGTGDRLLYANQAAEQFFDMGVG
                     LLLKQTLSDLLPFGSPLLQLVQQARNRGASIAERNVDLTTPKLGERLADVTLTPSGDE
                     EGVIILLQERSLAQRIDRQLLYRGAARSMSGMAAVLAHEIKNPLAGIRGAAQLLEDAI
                     SPNDRSLAQLICNEADRIRALIDRMEAFGDPMALVRGPVNIHEVLDRVRRVAETSFGR
                     RMRIVEVFDPSLPPVWGDRDNLIQAFMNIVRNAADAAPADNGEIVLTTAYRPGVRIAV
                     TGTGQKHRLSLPLEVTVRDNGVGIAHDLLPYIFDPFITTKTHGAGLGLPLVAKIINDH
                     GGVIECESQPRRTLFRVLLPVASAETQHHHSEPAHA"
     gene            1106..2557
                     /gene="ntrC"
                     /locus_tag="VGM72_03715"
     CDS             1106..2557
                     /gene="ntrC"
                     /locus_tag="VGM72_03715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEY4264400.1"
                     /translation="MPEGTILVCDDDAAIRTVLNQALGRAGYDVRTTANAAGLWRWIA
                     QGEGNLVITDVILPDENAFDLLPRIKRLRPELPVVVMSAQNNILTAITATERGAYEYL
                     PKPFDLKELITIVSRALAAPSRKRATAELEQGDERLPLIGRSAAMQEIYRIIARLTQA
                     DLTVMISGESGTGKELVARALHDYGKRRNGSFVAVNMAAIPKELIESELFGHERGAFT
                     GATGRGVGRFEQAEGGTLFLDEIGDMPLEAQTRLLRVLQQGEYTTVGGRVPIRTDVRI
                     IAATNRDLRQLIQQGLFREDLFYRLNVVPLRLPPLRERIEDIPDLVRHFLRKAEADGL
                     PLKRFDAPALERLKTHIWPGNVRELENLIRRLVVLNPDEVLNEDAVSGELAQLERSMP
                     VSDAEEASLASLIERHLAAHFAEYGERLPPSGLYDRVIRDVERPLIAISLAAARGNQI
                     RAAQLLGLNRNTLRKKIRELGVEVIRGLKSHGA"
     gene            2726..4996
                     /locus_tag="VGM72_03720"
     CDS             2726..4996
                     /locus_tag="VGM72_03720"
                     /inference="COORDINATES: protein motif:HMM:NF039586.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEY4264401.1"
                     /translation="MFRSRLIGGPFQLLRAASRSSLIALAVALAALISGILTYLGITG
                     LAPYNRTPAAMVVLIVVDLSLVLALAGLIAWRLTRLWTERRSGAAGSKLHLRLVAMFS
                     AIAVIPAILVAIFAAVSLNLGIEAWFSERVQTALANSVSVADAYVAEHKLVIRGDILA
                     MAFDLNNNAALLLSDPVKFRTTLENEAALRALPAVYIVDSTGRFLASAVQKDFIAKRP
                     QQSSPNPDQIAQANSSKQVVVTTDPKENVVSALVKLDAFVDAYLFVTRAVDPQVLEAQ
                     SRTQDAVSEYQRLNQNRSEIQLTFAALYVVVAMLILLAAVWLALWAASRIVTPIGRLA
                     GAAERVSEGDLGVRVAVGMDDDEIGALSETFNRMTSQLEGQRKELVDANFQLDSRRRF
                     TEAMLAGVSAGVLGLDGEGHITLINRMASRMLDAVPEDIEGRHYSECIPELAGLIRRA
                     IGEPTARAAGQVDVRRDGALRHLNVQVSSEAGDKIHGFVVTFDDITDLVSAQRTAAWA
                     DVARRIAHEIKNPLTPIQLSAERLKRKYSSEVSSDPEVFAQCTDTIIRQVGDIGRMVD
                     EFSSFARMPAPVVRREEAQELVRHAVFLQRVAHALIAFEVTAPVEPITFDCDGRLVSQ
                     ALINLLKNAAEAIAARMAAGQTMPGRIVLDLSAKNGRLIISVRDNGVGLPAENRHRLT
                     EPYVTTRAKGTGLGLAIVRKILEDHGGELVLEDVAGDSSGEATGAVVRMVFPLRRTGK
                     IEQGFMHETERVAGLA"
     gene            4965..6377
                     /locus_tag="VGM72_03725"
     CDS             4965..6377
                     /locus_tag="VGM72_03725"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY4264402.1"
                     /translation="MKQNGSLVSPEILVVDDEIDIRELMAGILADEGYETRVASDSDG
                     LLHAIGQRRPHLIVLDVWLQGSRLDGIQVLDILQREHPDLPVVIISGHGTIETAVAAI
                     KKGAYDFIEKPFKADKLCHTVRRALEASRLKREVEELKLKAGDDTELCGVSTAITQLR
                     QLVDKIAPTGSRVLITGPSGSGKEVTARLIHALSRRTNNAFIAINCAMMAPARIEAEL
                     FGVESEAGRKIGLLELAHGGTLFLDEAADMPLETQGKILRVLIDQTFQRVGGSTRVQV
                     DVRVISSSTRDLRQEIEAGRFREDLYHRLNVVPVRVPSLAERRDDVPLLVAHFMKRLA
                     AGSGLAIREISDEAMAVLQAYNWPGNVRQLRNIVERLLILATDDAAGAISADLLPSHF
                     NGSSSPWAQGQKPDLIISLPLREAREIFEREYLLAQINRFGGNISRTASFIGMERSAL
                     HRKLKSLGVSSARLDQVFNA"
     gene            6389..6721
                     /locus_tag="VGM72_03730"
     CDS             6389..6721
                     /locus_tag="VGM72_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264403.1"
                     /translation="MGDFLVKTVSAFVLCGGLIAGMSSVRAADAADTITANYVLQCQK
                     DQAACQAFTNDALRTLGQSRKICAPVPLSREQANQLVAWIVSRPQQASGHADDDIATA
                     AGALWPCK"
     gene            6718..7647
                     /locus_tag="VGM72_03735"
     CDS             6718..7647
                     /locus_tag="VGM72_03735"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF005209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HEY4264404.1"
                     /translation="MNAPSQRARPAPAGRVAYVNGRYLPHAAASVHVEDRGLQFADSI
                     YEVCAVLDGRLMDEAGHLDRMERSVGALAIAMPMARAPLRIVMREMIRRNLLKDGLLY
                     LQVTRGAHRRDHPMPAIHRSTLIMTARPINKTAVEKRRTDGIAVVTSPDMRWQRCDIK
                     TTGLLANVMAKTQARKAGAFEAWLVDRDGNVTEGASTNAWIVTEENVLVTRDLSPNIL
                     AGVTRMGVLSALAAEGFNAIEERAFTVEEARTAREAFVTSASGGVLPVISIDGHRIGE
                     GRPGPATWRIHELYARFACAAAEAAWDCASQLQ"
     gene            7737..7985
                     /gene="hfq"
                     /locus_tag="VGM72_03740"
     CDS             7737..7985
                     /gene="hfq"
                     /locus_tag="VGM72_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008385166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEY4264405.1"
                     /translation="MNDKPQNLQDTFLNAVRKSKISVTIYLVNGVKLQGNITWFDNFC
                     VLLRRDGQVQLVYKHAISTVMPMGPVQLQDQDAAQGEG"
     gene            8000..9361
                     /gene="hflX"
                     /locus_tag="VGM72_03745"
     CDS             8000..9361
                     /gene="hflX"
                     /locus_tag="VGM72_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY4264406.1"
                     /translation="MRSGKGHASRKGRRAIVLHIARKSGADSSRSEEARLQEAVALTA
                     AIGLDVAEGVIATIARATPATLIGTGKADEIKTLCHAVEPEVVIVNAQLSPVQQRNLE
                     KHWEAKVLDRTALILEIFGERARTREGRLQVELAHLTYQRSRLVKSWTHLERQRGGFG
                     FLGGPGETQIETDRRLIGERIAKIKRELEEVKRTRGLHRRARKKVPYPVVALVGYTNA
                     GKSTLFNRLTAAEVVAKDMLFATLDPTMRGLKLASGRAIILSDTVGFIADLPTQLVAA
                     FRATLEEVLEADVILHVRDAAHDESEAQKSDVLKVLHDLGVDKDQRRMIEVLNKIDLL
                     EPDVRQSLLTRNKVAGRMLTGEQLAVSALSGAGLEALTGSIDRLLGSQEQTMRLTLPV
                     DDGAGLAWAYEHGRVLERRQTDQGVYLVIAGDETIVDRFSRRFPGKLTIIDQDQRRKA
                     MSS"
     gene            9417..10361
                     /locus_tag="VGM72_03750"
     CDS             9417..10361
                     /locus_tag="VGM72_03750"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEY4264407.1"
                     /translation="MSLEPKEFSNLAALMKAIPDEQAAVDHFTAIRWKNGEFCPYCGG
                     TKVYHFADKRTHKCADKDCHQRFSIKVGTVFEDSKLGLRLWMLAVWYVSSHKKSIAST
                     QLAKDLGVTQKTAWFMMHRLRHAARTRSFNRPLRGTVEADEAFFGGKDKNQHADRRGL
                     KKKAIVVGMLERGGELRARIVDHLREAADVVAIHVQPGANLMTDEYTGYKRLRHDYEH
                     GFVAHSRGEYVKYPKASKSYDTEWGPLVHVNSIEGYWSQLKRQIYGIHHWVSAKHLGR
                     YIDESVWRYNRRDVGEAFRFNELLKITSGRLTYSDLIA"
     gene            10358..10594
                     /locus_tag="VGM72_03755"
     CDS             10358..10594
                     /locus_tag="VGM72_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264408.1"
                     /translation="MTARPPKEELALEMPFAEAMERFAGVIPAEMHANIAKSKKKKPP
                     SGKKKRKPPGGKAKNQNVVSLRERKNSLRRKGLA"
     gene            complement(11471..>12061)
                     /gene="mazG"
                     /locus_tag="VGM72_03760"
     CDS             complement(11471..>12061)
                     /gene="mazG"
                     /locus_tag="VGM72_03760"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEY4264409.1"
                     /translation="FHSQMAEEQQLFAFQDVVRAINAKMIRRHPHVFAEGAADTPQAV
                     AAAWEDIKRQERASKPAQAEGILDDVPGALPALMRAVKLQNRAAEVGFDWPSAMNVAD
                     KIAEETRELAEAAQTGIPAKVAEEFGDLLFAMANLARHLKLDPEDALRAANTKFIRRF
                     KMIEAGLKAKGRTPEDASLEEMEALWQDAKRAERPA"
BASE COUNT         2335 a         3623 c         3723 g         2380 t
ORIGIN      
        1 cccagagccg ctactgggca tagcggcatc attacaggcg cgcttccaat gcccatcctt
       61 ctggttggga caggcgaccg gctgctctat gccaatcagg cggcggagca attcttcgac
      121 atgggcgtgg gccttttgct gaagcagacg ctgagcgacc ttcttccatt cggcagtccg
      181 ttgctgcaac tggtgcagca ggcgcgcaac cgcggcgcct cgatcgccga gcgcaatgtc
      241 gatctgacaa cgcccaaatt gggggagcgt ttggccgacg tcacgctgac gccttccggc
      301 gatgaagaag gggtgatcat tcttttgcag gaacgcagcc ttgcccagcg catcgaccgg
      361 cagcttcttt atcgtggggc cgcgcgttcg atgagcggca tggcggccgt gctcgcccac
      421 gagatcaaaa atccgttggc gggcatccgg ggcgccgcgc agcttctgga ggacgcgatc
      481 tcgcccaacg accggtcgct tgcgcaactc atctgcaatg aagccgatcg cattcgcgca
      541 ctgatcgatc ggatggaggc gtttggggat ccgatggcgc tggtgcgcgg gccggtgaac
      601 attcacgaag tgctcgaccg ggtaagacgc gtggccgaga cgagcttcgg ccgccgcatg
      661 cgcatcgtcg aagttttcga tccgtcgctt cctcccgtct ggggcgatcg cgacaatctg
      721 attcaggcct tcatgaacat tgtgcgcaat gcggccgatg cggcacccgc cgacaatggc
      781 gagatcgtgc tcacgacggc ctatcgtccc ggtgtgcgga tcgcagtcac cggcaccgga
      841 caaaagcatc gcctgagtct gcccctcgaa gtcaccgtcc gcgataatgg cgtggggatc
      901 gcgcacgatc ttcttcccta tatcttcgat cccttcatca ccaccaagac ccatggcgcc
      961 gggctcggcc ttccgctcgt cgccaagatc atcaacgatc atggcggcgt catcgaatgc
     1021 gaatcccagc cgcgtcgcac gctctttcgc gtgctgttgc cggtcgcctc cgccgaaacc
     1081 caacatcatc acagcgagcc cgcacatgcc tgagggcaca attcttgtct gcgatgacga
     1141 tgccgccatt cgcactgtgc tcaaccaggc ccttggccgc gccggatacg atgtgcgcac
     1201 caccgcgaat gcggccgggc tgtggcgctg gatcgcgcag ggcgaaggca atctcgtcat
     1261 cactgatgtg atcctgcctg acgaaaatgc attcgatctt ctgccgcgga tcaagcggct
     1321 caggcctgag cttcccgtgg tggtgatgag cgcccagaac aatattctga ccgcaatcac
     1381 cgcgaccgaa cgcggcgcct atgaatatct gcccaagccg ttcgacctga aagagctgat
     1441 caccatcgta agccgtgcgc tggccgcccc gagccgaaaa cgcgcgacag ccgaattaga
     1501 gcagggcgac gagcgcctgc cgttgatcgg ccgttcggcc gcgatgcagg agatctatcg
     1561 catcatcgcg cgcctcaccc aggccgacct gaccgtgatg atctccggcg aatcgggaac
     1621 cggcaaggaa cttgtcgcgc gcgcgctgca cgattacggc aagcgccgca acggcagctt
     1681 cgtcgccgtg aacatggccg cgattcccaa agagctgatc gaaagcgaat tgttcggcca
     1741 tgagcgcggt gcgttcacgg gcgcgacggg gcggggcgtg ggccgcttcg aacaggctga
     1801 aggcggcacg ctgtttcttg atgaaatcgg cgatatgccg ctggaggcgc agacacggct
     1861 cctgcgcgtg ctgcagcagg gcgaatacac caccgtcggc ggccgcgtgc cgattcgaac
     1921 cgacgtgcgc atcatcgcgg ccaccaatcg cgatctacgc caattgattc agcaaggatt
     1981 gttccgggaa gatttgttct accggttgaa tgtcgtgccg ctgcgcttgc cgcctttgcg
     2041 cgagcgtatc gaagacatcc cagacctcgt gcgccatttc ctgcggaagg ccgaagccga
     2101 cggtctgccg cttaagcgat tcgatgcgcc cgcactcgaa cggctgaaga cccacatctg
     2161 gcccggcaat gtccgcgagc tggaaaatct catccgccgt ctggtggtgc tgaatccgga
     2221 cgaggtcctg aacgaggatg cggtctcggg cgaactggca cagcttgagc gcagcatgcc
     2281 ggtttcggac gctgaggagg cgagtctggc ctctttgatc gaacgtcatc tcgcggccca
     2341 tttcgccgaa tatggtgagc gtcttccgcc ctcgggcctc tatgaccggg tgattcggga
     2401 cgtcgagcgg ccgctgatcg cgatttcgct cgcggccgcg cgcggcaatc agattcgcgc
     2461 ggcgcaattg cttgggctca accgtaatac gctgcgaaag aagatccgtg agcttggcgt
     2521 ggaggtcata agggggctca aatcgcacgg cgcctgatct ttggccgaat atgcgcccga
     2581 tccatccagg ccggagccga tatcgtcggc ccggcatcac cgcgactttc ttgcaacatc
     2641 ctgtggcttt gctacactca cggcattcag gggatccatc gttctgcgtc gcgaggcctt
     2701 ccggccacat atggcaagca catccgtgtt ccgatcgcgt ctcatcgggg gacccttcca
     2761 actcctgcgc gccgcctcgc gttcctcgct gatcgcactc gccgttgcgc tggcggccct
     2821 gatctcgggc atcctgacct atctcggcat caccggtctc gccccctata atcgcacgcc
     2881 tgcggccatg gtcgtcctga tcgtggtgga cctttctttg gtgttggccc tcgcggggct
     2941 gatcgcctgg cgcctgacgc ggctgtggac cgagcgcaga agcggggcgg cgggttccaa
     3001 actccatctg cggctggttg cgatgttctc cgccatcgcg gtgatcccgg ccattctcgt
     3061 cgcaattttt gcggcggtgt cgctcaatct cggcatcgag gcctggtttt cggaacgcgt
     3121 gcagaccgca ctcgccaatt ccgtgagcgt cgccgatgcg tatgtcgccg agcacaagct
     3181 tgtcatccgt ggcgatattc tcgccatggc gttcgacctg aacaacaacg cggccttgtt
     3241 gctgagcgac ccggtcaagt tccggacgac tctggaaaac gaagcggcgc tgcgcgcgct
     3301 gccggcggtt tacatcgtcg attccaccgg gcgctttttg gccagtgccg tgcagaagga
     3361 ctttattgcc aaacggcccc agcaatcttc tcccaatccc gatcagattg cgcaggcaaa
     3421 ttccagcaaa caggtcgtgg tgacgaccga tccgaaagag aacgtggttt cggcgctggt
     3481 gaagctcgat gcgttcgtcg atgcttatct gtttgtcacg agagcggtcg atccgcaggt
     3541 cctggaagcc cagagccgca cgcaggatgc ggtttcggaa tatcagcgtc tgaaccaaaa
     3601 tcgttccgag atccagctca cgttcgccgc cctgtatgtg gtggtggcga tgctcattct
     3661 gctggctgcc gtatggctcg cgctgtgggc ggcgagccgt atcgtaacgc cgatcgggcg
     3721 gctggcggga gcggccgaac gcgtgtccga gggcgacctc ggtgtgcgtg tcgcggtcgg
     3781 aatggacgat gacgagatcg gcgcgctctc cgaaaccttc aaccgcatga cctctcagct
     3841 tgaggggcag cgcaaggaac tggtcgacgc caattttcag ctcgactcgc ggcgccggtt
     3901 taccgaggcg atgctcgccg gcgtttccgc gggcgttctg ggcttggacg gagaagggca
     3961 catcacgctg atcaaccgca tggcatcgcg gatgctcgat gccgtgccgg aggatatcga
     4021 gggccggcat tactccgaat gcattccgga gctggcgggt ttgatccggc gtgccatcgg
     4081 cgagcccacc gcccgtgcgg cgggccaggt ggatgtgcgg cgcgacggag cgttgcgcca
     4141 tttgaacgtg caggtgtcga gcgaagcggg agataagatc catggctttg tcgtcacctt
     4201 cgacgacatc accgatttgg tttccgcgca gcgtaccgcc gcctgggcgg acgtcgctcg
     4261 ccgcattgcg catgagatca agaatccgct gactccgatt cagctttcgg ccgagcggct
     4321 aaagcgcaaa tattccagcg aagtgtcgag cgatcccgag gtgttcgccc aatgcaccga
     4381 tacgatcatc cgccaggtcg gcgatatcgg ccgcatggtg gatgaatttt cttctttcgc
     4441 ccgcatgccc gcgccggtcg tccgccgtga ggaggcccag gaattggtgc gccatgccgt
     4501 cttcctgcag cgcgtggcgc atgccctgat cgcctttgag gtcaccgcgc cggtcgaacc
     4561 gattacattc gattgcgatg ggcggctggt gagccaggcg ctgatcaatt tgctgaagaa
     4621 tgcggccgaa gccattgccg cgcgcatggc ggccggtcag acgatgccag gacgaatcgt
     4681 tctcgatctg tcggcgaaaa acggacgtct catcatatcg gtgcgcgaca atggcgtggg
     4741 cctgcctgcg gaaaaccgtc accgcctgac cgaaccctat gtcacgacgc gagccaaggg
     4801 cacaggtctc gggctcgcca tcgtgcgcaa gattcttgaa gatcatggcg gcgaattggt
     4861 gctcgaggat gtcgccggcg attcatcggg cgaagctacg ggggcggtcg tgcgcatggt
     4921 ttttccgttg cgccgcacgg gcaaaatcga acaggggttc atgcatgaaa cagaacgggt
     4981 cgctggtctc gcctgagatt ctcgtcgtcg acgacgagat cgatatccgc gaactgatgg
     5041 cgggtattct tgccgatgaa ggttacgaga cgcgggtcgc ttcggactcg gatggtttgc
     5101 tccatgcgat cgggcagcgg cgtccccatc ttatcgtgct cgacgtctgg ctgcagggaa
     5161 gccgtctcga cggtatccag gtgctggata tcttgcagcg cgaacatccc gatcttccgg
     5221 tcgtcatcat cagcggccat ggcaccatcg aaaccgcggt cgcggccatc aagaaggggg
     5281 cctatgattt cattgaaaag cctttcaagg ccgacaaact gtgccacacc gtgcgccgcg
     5341 cgctggaagc ctcgcggctc aaacgcgaag tcgaggagct gaaactcaag gcaggagacg
     5401 atacggagct ttgcggggtc tccacggcga tcacgcaatt gcgccagctc gtcgataaga
     5461 tcgcgccgac cggaagccgc gtgctgatca ccggaccttc gggctccggc aaggaagtga
     5521 cggcgcggct tattcacgcg ctgtcgcggc ggaccaacaa tgccttcatc gcgatcaatt
     5581 gcgccatgat ggcaccggca cgcatcgagg cagaattgtt tggcgtggaa tcggaggccg
     5641 ggcgcaagat cggacttctg gaactcgccc atggcggcac gctgtttctc gacgaggcgg
     5701 cggatatgcc gctcgaaacg caaggtaaga tcctgcgcgt gctgatcgat cagacgttcc
     5761 aacgcgtcgg cgggtccacg cgcgtgcagg tggatgtgcg tgtgatctca tcctccacgc
     5821 gcgatctgcg ccaggagatc gaagccggcc ggttccgtga agatttgtac catcggctta
     5881 atgttgtgcc ggtccgcgtg ccttcgctcg ccgaacggcg cgacgacgtc ccgttgcttg
     5941 tggcccattt catgaagcgc ctggccgccg gatccggcct ggctattcgg gaaatctccg
     6001 atgaagccat ggccgtcctg caggcttaca attggccggg caatgtgcgc cagcttcgca
     6061 acatcgttga gcggcttctg atccttgcca cggacgatgc agcgggcgcg atttcggccg
     6121 atttgctgcc ctcccacttc aacggctcgt cctcgccttg ggcccagggg cagaaacccg
     6181 atctgatcat ctcgctgccg cttcgcgagg cacgcgaaat cttcgagcgc gaatatctac
     6241 ttgcgcagat taaccgcttc ggtggaaata tctcgcgtac ggcgtctttt attggcatgg
     6301 agcgctcggc attgcatcgc aagctgaaat cgctcggtgt atcctcggcc cgactcgatc
     6361 aggtttttaa cgcgtgatgc tatcccggat gggcgatttc ctcgtgaaaa ccgtttcggc
     6421 gttcgtgctc tgcggcggtt tgatcgctgg catgtcctcg gtccgcgccg ccgatgccgc
     6481 cgacaccatt acggcgaatt atgttctgca atgccaaaag gaccaggctg cctgccaagc
     6541 cttcaccaat gatgcgctgc ggactctcgg tcagagtcgc aaaatctgcg ctccggtgcc
     6601 cttaagccgg gagcaggcca accaattggt tgcctggatt gtctcccgcc cgcaacaggc
     6661 gagcggtcat gccgacgacg atatcgcaac agccgccggt gcgctctggc cgtgcaaatg
     6721 aatgccccgt cacaacgcgc ccggccggcg ccggccggac gcgtcgccta tgtgaacgga
     6781 cgctatcttc cgcacgcggc cgcgtcggtg catgtggaag atcgcggctt gcaatttgcc
     6841 gattcgatct acgaggtttg cgccgttctc gacggccgcc tgatggatga ggcaggccat
     6901 ctcgacagaa tggagcgaag cgtcggcgcg cttgcgatcg ccatgccgat ggcacgcgca
     6961 ccgctgcgca tcgtcatgcg ggaaatgatc cgccgaaact tgctgaaaga cggcttgctc
     7021 tatctgcagg tcacgcgcgg cgcgcacagg cgcgaccatc cgatgcctgc catccaccgc
     7081 agcacgctca tcatgacggc gcggccgatc aacaaaaccg ccgttgaaaa gcggcgcacg
     7141 gacgggatag ccgtggtcac atcgcccgat atgcgatggc agcgctgcga tatcaagacg
     7201 accggcctgc ttgccaatgt gatggctaag acgcaagcgc gcaaagcggg cgcctttgaa
     7261 gcctggctgg tcgatcgcga tgggaacgtg accgagggcg cgtccacgaa cgcctggatc
     7321 gtcacggaag aaaatgttct ggtcacgcgc gatctgtcac ccaatatcct tgccggcgtg
     7381 acccgaatgg gcgtcctgag cgcgctggcg gccgagggtt tcaacgccat cgaggagcgc
     7441 gctttcacgg ttgaggaagc ccgcacggca cgggaagcct ttgtcacctc ggcgtccggt
     7501 ggggtactgc cggtcatctc catcgatgga caccggatcg gcgagggccg tccgggcccg
     7561 gctacatggc gcatccatga gctttacgcg cgatttgcct gtgcagcggc cgaagccgcc
     7621 tgggattgtg cttcgcaatt gcaataaaca ccgatattgt gaatgaaggc ggaaagcgta
     7681 ttccgtcctg acgtcctcgc agcggcgtta caacgcaaaa caaatggggt tcacccatga
     7741 acgataaacc acaaaatctt caagacactt ttcttaacgc cgtccgtaag agcaagatct
     7801 ccgtcaccat ttatctggtc aatggcgtga aattgcaggg caatattacc tggttcgaca
     7861 atttctgcgt gctcctgcgc cgtgatggac aggtgcagct cgtctacaag catgccatct
     7921 ccacggtgat gccaatggga ccggtccaat tgcaggacca ggatgccgcc caaggggaag
     7981 gctgacgcgc gagacatcgg tgagatccgg taagggccac gcctcgcgca aggggcggcg
     8041 ggccatcgtg cttcacatcg cgcggaaatc gggcgcggac tccagccgga gcgaggaagc
     8101 gcgcctgcaa gaggctgtcg cgctgacggc cgcgatcggt ttggatgtcg cagaaggcgt
     8161 gattgcgacg atcgcgcgcg cgacgcctgc gacactcatc ggcacgggaa aggccgatga
     8221 gatcaagacg ctttgccatg cggtcgagcc cgaagtggtg atcgtcaatg cccagctttc
     8281 tccggtccag cagcgcaatc tggagaaaca ttgggaggcc aaggttctgg accgcacggc
     8341 gctgatcctg gagattttcg gcgagcgggc gagaacgcgg gaaggacgcc ttcaggtcga
     8401 gcttgcccat ctgacctatc agcggtcgcg tctggtcaag agctggaccc atctggaacg
     8461 ccagcgcggc ggcttcgggt ttctgggcgg cccgggcgaa acgcagatcg aaaccgaccg
     8521 ccggctgatc ggagaacgga tcgccaagat caagcgcgag ctggaggagg tcaagcgcac
     8581 acgcgggctg caccgccgcg cccggaagaa agtgccttat cccgtggttg cgctcgtcgg
     8641 ttacaccaat gccggaaaat cgacgctttt caaccgtttg accgctgcgg aagtggtggc
     8701 caaggacatg ctgttcgcga ccctcgatcc cacgatgcga gggctgaaac tggcctcggg
     8761 gcgcgcgatc attctctccg ataccgttgg tttcattgcc gatcttccca cccagctggt
     8821 ggcggccttt cgcgcgaccc tggaagaggt gctggaggcc gacgtcattc tgcatgtgcg
     8881 cgatgccgcc cacgatgagt cggaagccca aaaatccgat gttctcaagg tgttgcacga
     8941 tcttggtgtg gacaaagatc agcgcaggat gatcgaagtg ctgaacaaga tcgacctgct
     9001 ggaacccgat gtccgccagt cgctccttac ccgcaataag gtggccggac gcatgttgac
     9061 aggcgaacaa ttggcggttt ccgcgctttc cggtgccggt ctggaggcgc tgacgggttc
     9121 tatcgaccgc ctgttgggaa gccaggaaca gaccatgcgg ctgacgctgc cggtcgacga
     9181 cggcgccggt ctggcatggg cgtatgaaca tggccgcgtg ctggaacggc gtcagaccga
     9241 ccagggcgta tatctcgtca ttgccggcga cgagaccatt gtggatcgtt tctcgcggcg
     9301 cttccccggc aaactgacga tcatcgatca ggatcaacgg cgtaaagcga tgtcctcgta
     9361 attctatcca gggatttata tagtatagtg acctagtgaa atcagggacg attgctatgt
     9421 cattggaacc gaaagagttt tctaatctcg ccgcgctgat gaaggccatc ccagacgaac
     9481 aagcggctgt ggatcatttt acagccatcc gttggaagaa tggcgagttc tgcccctact
     9541 gtggtgggac caaggtttac cacttcgcgg acaagcgcac ccacaagtgc gccgacaagg
     9601 attgccatca gagattttcg atcaaagtcg ggactgtctt tgaagactcg aaattgggtc
     9661 tacgcctttg gatgctggcg gtttggtatg tcagctctca caagaaaagc atcgccagca
     9721 cccaactcgc gaaagacctt ggcgtcacgc agaagaccgc gtggttcatg atgcaccggc
     9781 tacgccatgc cgcccgcacg cggtccttca atcgccctct gcggggcacg gttgaggccg
     9841 atgaggcttt cttcggtggc aaagataaga atcagcacgc ggacaggcgc ggcctcaaaa
     9901 agaaggcaat cgtggtcggc atgttggagc gcggagggga attgcgggcg cgcatagtgg
     9961 accatcttcg cgaggcggca gacgttgtag ccatccatgt acagccgggc gcaaacctga
    10021 tgaccgatga gtacacgggc tataagcgcc tgcgccacga ttacgagcat gggtttgtcg
    10081 cccattcccg tggcgaatat gtcaagtatc cgaaagcgtc aaaatcctat gatacggagt
    10141 ggggtccgct ggttcacgta aacagcattg aaggctattg gtcccaattg aagcgccaga
    10201 tttacggcat ccatcattgg gttagcgcga agcacttggg tcgctacatc gatgagagcg
    10261 tatggcgtta taatcgccgt gacgttggcg aagccttccg gttcaatgaa ctgctcaaga
    10321 tcacaagcgg caggctgaca tacagcgact taatcgcatg accgcaaggc cgcccaagga
    10381 agaactagcg ctagagatgc ccttcgcaga ggcgatggaa cgctttgccg gtgtcatacc
    10441 ggcagagatg cacgccaaca ttgccaagtc gaaaaagaag aaaccgccga gcggcaagaa
    10501 gaaacgaaaa ccgcccggcg gaaaggctaa aaatcagaac gttgtgtcat tgcgtgagcg
    10561 caagaacagt cttcgtagga agggccttgc gtgaaggata ccgcctccaa tagccccgga
    10621 cttgttcgag gcagccaaag cggatgcggt agtgtggttc gacccagagg aaggccgtga
    10681 ggatagacat ggttttcacc tgttgtttgg acaggcgcgg tgcgagagtg gtgtcggtgg
    10741 ttgaagccca gtggacacca gctcgcactc gcgcatgtcg cgtgagtgcc gaaaaatgct
    10801 gtgtgtccga ccgctatcgg gacttcatca aagggcgcta tccacatgcg cagactgcaa
    10861 gcctttgaag ttactcaacc actagtcgga gctttcagag tctccagggc cgatgctttt
    10921 cgcaccagcc atccaaactc ttccggcgag tcgaaaactc gcacatttcc cgcccttggt
    10981 caaactcgta tgtaaacgcc tctatccaca ggcatgtggg ttttgtggat gatcgcacgc
    11041 gcttgagcta ttcccgcgaa ctgcaagctg cgggagggtg aggggatggt gtgaggcgcc
    11101 ggtcaggcgg tccacaattg tgtttcaaaa aaagatgccg atccggcacc ccacacccgg
    11161 cgattttagg cgcggcgctt cccgtttcgc cggccgcaaa cgcgaacggc ttatcggcaa
    11221 cccgtcctcc cgccagccct tcctgtcgga atggtagcgg gtcggctgga aagcgtgcct
    11281 tcgcatcctt ctttgacagg cccccttagg gtcatcctgc cgccgaagtt tttcccgtcc
    11341 gtccgttacc cggccgggag atgcaaacgc cggccgaaac ccacattgaa gacggtcgac
    11401 aacgcccctt atcaggctcg gctttgtcga tgtgggacgg cggattcgaa agacaagagc
    11461 agcgtcacgc tcaagccggc cgctcggcgc gtttggcgtc ctgccacagc gcctccatct
    11521 cctccagcga ggcgtcctcc ggggtgcggc ctttagcctt aagacccgct tcgatcatct
    11581 tgaagcgccg tataaatttc gtattggcgg cacgcagcgc gtcttccgga tcgagcttca
    11641 gatggcgggc cagattggcc atcgcaaaaa gcaaatcgcc gaattcctcc gcgaccttgg
    11701 cggggatgcc ggtttgtgcc gcctcggcca attcacgggt ttcctcggcg attttgtcgg
    11761 ccacattcat cgcactcggc caatcgaatc cgacctcggc cgcgcggttc tgcaatttga
    11821 ccgcgcgcat caatgcgggc agggcgcccg gcacatcgtc caggatgccc tcggcctggg
    11881 cgggcttgga ggcgcgttcc tgccgtttga tgtcctccca agcggccgcc acggcttgcg
    11941 gcgtatcggc ggcgccttcc gcaaagacat gggggtggcg gcggatcatc ttggcgttga
    12001 tggcgcgcac cacgtcctga aaggcgaaaa gctgctgttc ctcggccatc tgcgaatgga
    12061 a
//
LOCUS       DASYEG010000099         5031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3364910, whole genome shotgun sequence.
ACCESSION   DASYEG010000099 DASYEG010000000
VERSION     DASYEG010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3364910"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VGM72_03765"
     CDS             complement(<1..761)
                     /locus_tag="VGM72_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEY4264410.1"
                     /translation="MRLARQLWFQVLVAMAAGAMLGYARPDFGVVMQPLGDLFVNLIR
                     MIIGPVIFCTVVHGIAAMNDMKRVGRVALKALIWFEAVTLAALVFALVGVNLWQPGAG
                     MNIDPSALDANSVRSYAAAAQHQTFSEFLLHIVPSTFVSAFAQGDVLQVLFVSVLFAF
                     ALALIGEPAKPVLDVIGRLSQIFFRIVGLIMWVAPIGAFGALAFTVGRFGASSLLSLG
                     GLIVEFYVVCLAFVLIVLGGVARALGVSMFRLLYY"
     gene            complement(764..1798)
                     /locus_tag="VGM72_03770"
     CDS             complement(764..1798)
                     /locus_tag="VGM72_03770"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4264411.1"
                     /translation="MIIDCHGHYTTEPKPLMEFRQKQTAAVKDHAALPAPSSVKISDD
                     DFRASVEGNQLRIQRERGTDLTLFSPRASGMGHHIGDAKTSLEWSRLSNDCIQRVATL
                     FPNNFIGVCQLPQSPGVAPENCIPELERCINELGFVGCNLNPDPSGGSWTAPPLTDRS
                     WYPLYEKLCDLQVPAMVHVSMSCNPAFHTTGAHYINADTTAFMQFIQGDLFKDFPDLK
                     LIVPHGGGAVPYHWGRYRGLAQNLNRPPLDEMMRNNVFFDTCVYHQPGIDLLTKLIPA
                     DNILFASEMLGAVKGDDPTTGKPYDDTKRYIDASALSAGDKQKIFEGNARKVFPRLGP
                     KLDKIAAARK"
     gene            complement(1813..2721)
                     /locus_tag="VGM72_03775"
     CDS             complement(1813..2721)
                     /locus_tag="VGM72_03775"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HEY4264412.1"
                     /translation="MPAFSRITLIGFGEVGQTLGEDLLAAGAKITAFDILFAKPESAP
                     ASVLRRIPVSMAANARDAVADAELVIAAVTAASDLEAVQAAAPGLPRGAFYLDVNSVS
                     PGMKRSCAEIIEAAGGHYVEAAVMTPIAPKRIASAMLLGGPYARTFAERARPLGFTGA
                     KPFSDIIGQASATKMCRSVIIKGVEALLCESMLTARHYGIEKHVLDSLSDLLPVGDWE
                     RLARYFISRSLEHGTRRAEEMRESAKTVAEAGLEPLMALATAEREDWSAAHKYALAHI
                     DDLGAMLDAIRNNFDTEAAASLKAAE"
     gene            complement(2726..3415)
                     /locus_tag="VGM72_03780"
     CDS             complement(2726..3415)
                     /locus_tag="VGM72_03780"
                     /inference="COORDINATES: protein motif:HMM:NF006731.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HEY4264413.1"
                     /translation="MSVVVRNIRRVDPAVVEGISGIGVATVHEAQGRKGLLASTMRPI
                     YRPCHIAGAAVTCEMAPGDNWMLHVAVEQCQKGDILVATPTSPCEDGYFGELIATQLQ
                     TRGVAGLILEAGCRDVSELTKMGFPVWSRAVFAQGTVKETVGNVNLPVVCAGQLINPG
                     DLIVADDDGVVVVRRKEAANILQLSREREEKEAKSRQRYLKGELSLDVNNMRAKLAEK
                     GLQYLDNPPED"
     gene            complement(3516..4100)
                     /locus_tag="VGM72_03785"
     CDS             complement(3516..4100)
                     /locus_tag="VGM72_03785"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY4264414.1"
                     /translation="MSWKYLLTAASAAVMTVPAHDASAQLQNYVIQGEMAKKIQQKSE
                     ISLDTAKKIASGCEAFAKANQASAAIVIVDMFGQEVFFERLDGAFGLTQLVAARRKAE
                     TALSSRRTSREEMNRVLKGQTTEFHEGFYNDMFAVPGGVPIVVDDQMLGAVGVGGSND
                     DEACARAGLDAVHVKQPPRADILPRRYGSAAGGG"
     gene            4224..5030
                     /locus_tag="VGM72_03790"
     CDS             4224..5030
                     /locus_tag="VGM72_03790"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4264415.1"
                     /translation="MAFIEANGVSFHYRLEGPGNTFGNTSVGPTMIFLHEIGGSLDSW
                     DGVTPDLAKRFTVFRYDQRGFGLSEKTRAPYSIETLTDDLQSLIDALKLTPPFHVVSL
                     AASSMQALKFHARNPQKIGSLVFCNPAPGVDPSRADALNAVAAKAEAEGIRGIIPGML
                     DKSYPPELSTREAYENYRGRYMANDPVGFGNAFRMMAGTNLINLLETIKVPSLVIAGR
                     QDFIRAVAVTEGLAKKIPAARFEIIDAGHFMPTTSPGALLALLQDFWKSA"
BASE COUNT         1011 a         1597 c         1493 g          930 t
ORIGIN      
        1 atgtaataca gcaatcggaa catgctgacg cccagcgcgc gcgccacgcc gccgagaacg
       61 atcagcacga aggcgaggca aacgacatag aactccacaa tcaacccgcc caaggagagc
      121 agcgatgagg caccaaaacg ccccactgtg aaggcaagcg cgccgaaggc gccgatggga
      181 gccacccaca tgatgaggcc gacaatgcga aagaatatct gcgacagccg tccgatgaca
      241 tcgagcacag gcttggccgg ttcgccaatc agcgccaagg caaaggcgaa caggacagag
      301 acgaacagca cctgcagtac atcgccttgc gcaaaggcgg agacgaaggt ggacggtacg
      361 atatgcagca ggaattccga gaatgtctga tgttgggccg ccgccgcata gctgcgcacg
      421 gaattcgcat cgagtgcgga aggatcgatg ttcatgccgg cgccgggctg ccagagattg
      481 acgccgacga gcgcgaaaac aagtgcggcg agcgtaacgg cttcgaacca gatgagcgcc
      541 ttcaacgcca cgcgccccac ccgcttcatg tcgttcatcg cggcgatgcc gtgcaccacg
      601 gtgcagaaga tcaccgggcc gatgatcata cggatgagat tgacgaagag atcgcccagg
      661 ggctgcatca ccacgccgaa atcgggtcgc gcatagccca gcatcgcgcc cgccgccatg
      721 gccacgagca cctggaacca gagctggcgc gccaagcgca tgcttatttc cgggccgcgg
      781 cgattttgtc gagcttcggc cccagccgcg gaaacacctt gcgggcgttg ccttcgaaga
      841 tcttctgctt gtcgcccgcc gaaagcgccg atgcatcgat atagcgcttg gtatcgtcat
      901 acggtttgcc ggtcgtcgga tcgtcgccct tcaccgcgcc cagcatttcg gaggcgaaca
      961 ggatgttgtc cgccggaatc agcttggtca aaagatcgat gcccggctgg tgatagacgc
     1021 aggtgtcgaa gaagacattg ttgcgcatca tctcatccaa aggggggcga ttcagattct
     1081 gcgccaggcc ccgatagcgt ccccaatgat agggcacagc acccccgcca tggggcacga
     1141 tcagcttcag gtccgggaaa tccttgaaga gatcgccctg gatgaattgc atgaaggccg
     1201 tcgtgtcggc attgatgtaa tgcgccccgg tggtgtggaa cgccggattg cacgacatgc
     1261 tgacatgcac catcgccggc acctgcagat cgcagagctt ttcgtacagc ggataccaag
     1321 agcggtcggt caggggcggc gccgtccagc ttcccccgga cggatcggga ttgagattgc
     1381 agccgacgaa gccgagctcg ttgatgcagc gttccaactc ggggatgcag ttttccggcg
     1441 caacgcccgg actttgcggc aattggcaga cgccgatgaa attgttcggg aacagcgtgg
     1501 cgacgcgctg aatgcaatca ttggaaagcc gcgaccattc aaggctggtc ttggcatcgc
     1561 cgatatgatg gcccatgccc gaggcacgcg gactgaacag cgtcagatcc gtgccgcgct
     1621 cgcgctggat gcggagctga ttgccctcga cgctggcgcg gaaatcatcg tcgctgatct
     1681 ttaccgacga cggagcgggg agagcggcgt ggtccttcac cgccgccgtc tgcttctggc
     1741 ggaattccat gagcggcttg ggctcggtcg tgtaatggcc gtgacaatcg atgatcatga
     1801 ataccccata gattattccg cggccttcaa cgatgcggcc gcctctgtgt cgaaattgtt
     1861 gcgaatggca tcgagcatgg cgcccagatc gtcgatatgc gccagcgcgt atttatgcgc
     1921 cgccgaccaa tcctcgcgtt cggcagtcgc caatgccatc aggggctcga gccccgcctc
     1981 ggcaaccgtc ttcgccgatt cgcgcatttc ctcggcgcgg cgcgtgccat gttcgagcga
     2041 gcgcgagatg aaataacgcg ccagtctttc ccaatcgcca acgggcaaaa gatcggacag
     2101 cgaatccagc acatgtttct cgatgccgta atggcgcgcc gtcagcatgg attcgcacag
     2161 cagcgcttcg acgcccttga tgatgacgct gcggcacatt ttggtggccg aagcctggcc
     2221 gatgatgtcg gaaaacggct tggcgccggt gaaaccgagc gggcgtgcgc gttccgcaaa
     2281 ggttcgggca tagggcccgc cgagcagcat cgccgatgcg atccgcttgg gcgcgatggg
     2341 ggtcatcacc gcggcctcca cgtaatgccc gcccgcagcc tcgatgattt cggcacaact
     2401 tcgcttcatg ccgggcgaga cggaattcac gtcgagataa aacgccccgc gcggcaggcc
     2461 gggcgccgcc gcttgcaccg cctccaaatc ggaggccgcc gtcaccgcgg cgattacgag
     2521 ttcggcgtcc gccaccgcat cgcgggcgtt tgccgccata gaaacgggaa tccggcgaag
     2581 caccgatgcg ggcgcagatt cgggtttcgc gaacaggatg tcgaaggcgg tgattttcgc
     2641 gccggccgct aacagatctt cgccgagtgt ctgcccgacc tcaccaaatc cgatcagcgt
     2701 gatgcgggag aacgccggca ccgcctcaat cctccggcgg attgtcgaga tattggagtc
     2761 ccttctccgc cagcttggcg cgcatattgt tcacgtcgag actcaactcg cccttgagat
     2821 aacgctggcg gcttttggcc tccttctctt cgcgttcgcg gctcagctgc aggatgttgg
     2881 ccgcttcctt gcgccgcacc accaccacgc cgtcatcgtc cgcgacaatg agatcgccgg
     2941 gattgatcaa ctgccccgcg cagaccaccg gcagattgac gttgccgacc gtctccttga
     3001 cggtgccttg cgcaaagacc gcccgcgacc agaccggaaa acccatcttg gtcagctccg
     3061 acacgtcccg gcagccggcc tccaaaatca gaccggcgac accgcgcgtc tgaagctggg
     3121 tcgcgatcag ctcgccgaaa taaccgtcct cgcaggggct ggtgggcgtc gcgaccagga
     3181 tgtcaccctt ttggcactgc tcgacggcga catgcagcat ccaattatcg ccgggcgcca
     3241 tttcgcaggt gacggccgcg cccgcgatgt ggcagggccg atagatcggc cgcatggtgg
     3301 aggcaagcaa acccttgcgc ccttgcgctt catggacggt cgcaacaccg atcccgctta
     3361 tgccttcgac cacggccggg tccacccggc ggatgtttcg gaccaccacg ctcacgcgaa
     3421 ttctcccttt gtttttaagt ctttagactt atcccactgc ctccgtaaag gcgcatgaac
     3481 attcataggc ccgccaggat cgaacctcaa taggatcagc cgccgccagc cgccgagccg
     3541 tatcgccgcg gcaggatgtc cgcccgcggc ggttgtttca catgcaccgc atcgaggccg
     3601 gcccgcgcgc acgcttcatc gtcgttcgat ccgccgacgc cgaccgcacc gagcatctga
     3661 tcgtcgacca cgatcggcac tccgccgggc acggcgaaca tatcgttgta aaagccttcg
     3721 tggaattccg tggtctggcc tttcagaacc cggttcatct cctcgcggct ggtgcggcgc
     3781 gagctgagcg cggtttccgc cttgcgtcgc gccgcaacga gttgggtcag cccgaacgcg
     3841 ccgtcgagcc gttcgaaaaa gacttcctgg ccgaacatat cgacgatgac gatggcggcg
     3901 gacgcctggt tcgctttggc aaaggcttcg cagcccgaag cgatcttctt ggcagtatcg
     3961 agcgaaattt cgctcttctg ctgaatcttc ttcgccatct cgccctggat gacataattc
     4021 tgaagttgcg ccgacgcatc gtgcgccggc acggtcatca cggcagcgct cgcggccgtc
     4081 aaaagatatt tccagctcat gatgttctcc caacgattat tgccctcagt gtgatgtaga
     4141 cgtccgcctt cggcaataat ggcgcttgtt tgtccggcga agaggcgcaa tagacacgag
     4201 cgcataaagc gacaggagac ggcatggcgt tcatcgaggc caatggggtc agctttcatt
     4261 accggctcga aggcccgggc aatacattcg gcaacacatc cgtcgggccg acgatgatct
     4321 tcctgcatga gatcggcgga tcgctcgaca gttgggacgg cgtgacgccc gacctggcga
     4381 aacgtttcac ggtcttccgc tacgaccagc gcggcttcgg gctgagcgag aaaacgcgcg
     4441 ccccctattc catcgagacg ctgaccgacg atcttcagag cctgatcgac gcgctgaagc
     4501 tcacgccccc ctttcatgtg gtgtcgttgg ccgcctccag catgcaggcc ctgaaatttc
     4561 atgcgcgcaa tccgcaaaag atcggctcgc tcgtgttctg caaccccgca cccggcgtag
     4621 acccctcacg cgcggacgcg ctcaatgcgg tcgccgcgaa agccgaagcc gaaggcatcc
     4681 gcggcatcat tccggggatg ctggacaaat cctatccgcc cgaattgtcg acccgcgagg
     4741 cttacgagaa ttatcgcgga cgctatatgg cgaacgatcc cgtcggcttc ggcaatgcgt
     4801 ttcgcatgat ggccggcacc aatctcataa atctgctcga aacgataaaa gtgccgtctt
     4861 tggtcatcgc cggacggcag gattttatcc gcgcggttgc ggtcaccgag ggtctggcca
     4921 aaaagattcc ggctgcgcgc ttcgaaatca tcgacgccgg ccatttcatg ccgaccactt
     4981 cacccggcgc gttgctcgcg ttgctccagg atttctggaa aagcgcctaa c
//
LOCUS       DASYEG010000100         3561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1914877, whole genome shotgun sequence.
ACCESSION   DASYEG010000100 DASYEG010000000
VERSION     DASYEG010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1914877"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VGM72_03795"
     CDS             <1..220
                     /locus_tag="VGM72_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264416.1"
                     /translation="QSSDMPGDRFNPGCKRGIRVGSTKDGKVVAYIPPPPPADPKMQP
                     AIGVAVDKNGNIFAASDDQHNVVKWVKN"
     gene            complement(333..1232)
                     /locus_tag="VGM72_03800"
     CDS             complement(333..1232)
                     /locus_tag="VGM72_03800"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4264417.1"
                     /translation="MIRPLIAGLFIVAAVSSNASAQSCTGNPVAVQILGSGAPGFVKD
                     RANTSYLLWVGNQAKILLDAGGGAYVRFGQSGAKFGDLSMILVSHLHPDHASDLPGIL
                     WSGRMMRSDSLPIVGPSGNEAAPDFSVFLKRLFDAKTGSFEILNSVMPGASGMGVKLD
                     TSVVDVTKPDATTVFDRDGIKVTAMGIPHGNLPTLAYRVETQGLSVVFSSDQNGTNPR
                     FPGFAKDANLLVMHLAIEVGAKNPNQALPALVGTVAQSAHPGRLILSHIANFDLDAAV
                     ADVKKGYTGPLVVGADLQCTQVK"
     gene            complement(1258..1953)
                     /locus_tag="VGM72_03805"
     CDS             complement(1258..1953)
                     /locus_tag="VGM72_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264418.1"
                     /translation="MKRGFISVVAAVGFFAAAPLAAQQETPVPMMKAPFHLPVFHNDY
                     VTVLNVNVLPGHTTGYHIHTFDQVSVNIGAADMTNQNFGSNEVSKPQRGEAGRATFTD
                     YVKNGMHTHKAVNVGPTPFHNISTLLTGKASGILPAARGGAYKQEIDNAKVRVWRLVL
                     DPGQSTPAITQNAPGMRVIVRGGEFTEIVPGQPDRAMGPSTGDFFWQDAGATRAIRNT
                     GTTPVEFVEFEIK"
     gene            complement(2139..>3561)
                     /locus_tag="VGM72_03810"
     CDS             complement(2139..>3561)
                     /locus_tag="VGM72_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4264419.1"
                     /translation="LGDDTFLSDVPARLDRLRWGRFHWLVVVALGITWILDGLEVTLV
                     GSLAGVLTQGATLALTSTQIGLTGSAYIIGAVLGALLFGDLTDRFGRKKLFTVTVGLY
                     VVATVATGFAWDFWSFALFRVLTGAGIGGEYAAINSAIQEFVPARYRGRTDIIVNGSF
                     WVGAAIGALASLIVLNPAVLPIAYGWRLAFIVGGVLGVFIVWLRHFVPESPRWLMTHG
                     RIAEAERVIGEIEVREGIAPPRTACVPMSFAPYRGTRFGDVVRALLRHYPKRTILGLV
                     LMATQAFCYNAIFFTYALVLTNFYGVPASDIGWFILPFALGNFLGPLTLGHLFDTLGR
                     KIMITATYAVSGILMAITGVLFAAGTLSAAEQTALWTINFFFASCGASAAYLTVGEAF
                     PLEVRARAISIFYAVGTGIGGIAGPALFGALIEAGSRREILWGYLAGGALMLAAAATE
                     AVLGIKAERVALEDVASPLSRVR"
BASE COUNT          744 a         1108 c         1077 g          632 t
ORIGIN      
        1 ccaatcctcc gacatgccgg gcgaccgctt caatcccggc tgcaagcgcg gcatccgcgt
       61 ggggagcacg aaagatggca aggtggttgc gtatattccc ccgccgccgc ccgccgatcc
      121 gaaaatgcaa cccgccatcg gtgtcgcggt ggacaagaac ggcaacatct tcgccgcctc
      181 ggacgaccag cataacgtcg tgaagtgggt gaagaactag gtgaagtggg tgaagaacta
      241 ggattcgaca cgaaggacac acaggctgca tcaaggcctg cgccttcgtg cagcttcgtg
      301 cccttagtgt ttcaacctta gcgagctgag cgtcacttca cctgcgtgca ttgcagatcg
      361 gcgccgacga cgagcgggcc ggtatagccc ttcttcacat ccgccacggc cgcatcgaga
      421 tcgaaattcg cgatgtggct gaggatcagg cgtcccggat gcgcgctctg cgcgaccgtg
      481 ccgaccagcg cgggcaaggc ctgattggga ttttttgcgc ccacttcgat ggccagatgc
      541 atgaccagaa gattcgcgtc cttggcgaat ccggggaatc tcggattggt gccgttttga
      601 tcggagctga aaacgaccga cagcccttgc gtctcgacgc gataggccag ggtcggaaga
      661 ttgccgtgcg ggattcccat cgccgtcacc ttgatgccgt cgcggtcgaa caccgttgtg
      721 gcgtcgggct tggtgacatc gacgacgctc gtatccaatt tgacgcccat cccggacgcg
      781 cccggcatga ccgagttcag gatttcgaac gagccggtct tcgcgtcgaa caggcgtttg
      841 aggaataccg aaaagtccgg cgccgcctca ttgcccgacg gcccgacgat ggggagagaa
      901 tcgctgcgca tcatgcggcc cgaccagagg atcccgggca aatccgaagc atgatcgggg
      961 tgaagatgac tgaccaggat catcgatagg tcgccgaact tcgcgcccga ctgcccaaag
     1021 cgcacatagg cgccgccgcc ggcatcgagg aggatcttcg cctgattgcc gacccacaaa
     1081 agataagagg tgttggcacg atccttgacg aaacccggcg cgccggagcc cagaatctgc
     1141 acggccacag gattgccggt gcagctttgc gcagatgcgt tcgaagaaac cgcggcaacg
     1201 ataaaaagtc ctgcgatcag tggacggatc atacgcacct cccgttttgt ttctgtatta
     1261 tttgatctcg aattccacga actccaccgg cgtcgtgccg gtgttgcgga tggcgcgggt
     1321 ggcgcccgcg tcctgccaga agaaatcgcc cgtgctgggt cccatcgcgc ggtcaggctg
     1381 acccggcacg atttcggtaa attcgccgcc acggacgatg acccgcatcc ccggcgcgtt
     1441 ctgcgtgatc gcgggcgtgg attgcccggg gtctaaaacc aagcgccata cacgcacctt
     1501 tgcgttgtcg atctcctgct tgtaggcacc gccacgggcc gcgggcagaa tgccgctcgc
     1561 cttgccggtg agcagcgtcg aaatgttgtg gaacggcgtc ggccccacat tgaccgcctt
     1621 gtgcgtgtgc atgccgtttt tgacatagtc ggtgaatgtg gcgcgtcccg cctcgccgcg
     1681 ttggggcttg ctcacttcgt tcgagccgaa attctgattg gtcatgtcgg cggcgccgat
     1741 attcaccgac acctgatcga aggtgtggat gtgatagccg gtggtgtggc ccggcagcac
     1801 attcacgttc aacacggtca cataatcatt gtggaacacc ggcaaatgaa acggcgcctt
     1861 catcatcggc acgggcgttt cctgctgcgc ggccaacggc gctgccgcaa agaatccgac
     1921 tgctgcgaca accgatatga acccccgctt catgcaccgc tcctgccctt ggatttgctg
     1981 ccgacgctag cgattttccg ggctcctcgc cagcacccac aattcgtcat ggccgggctt
     2041 gacccggcca tggtgaaaat ccgttcgagg cggatttcac attcgcgtgg ctcttgagca
     2101 ccttgtgggt catccggtcc tgcacgcacc cttccccgtc accgtacgcg tgacaggggc
     2161 gatgccacat cctcaagcgc aacgcgttcg gctttgattc ccagaaccgc ttcggtcgcg
     2221 gcggccgcga gcatcagcgc gccgcccgca agatatcccc acaaaatctc ccggcgcgag
     2281 cctgcctcga tgagcgcgcc aaacaatgcc gggcccgcga tgccaccgat cccggtgccg
     2341 actgcgtaga agatcgaaat ggcgcgggcg cgcacttcca gcggaaaagc ttcaccaacc
     2401 gtcagatagg ccgcgctcgc gccgcaggac gcgaagaaga agttgatcgt ccatagcgcg
     2461 gtttgctccg cggcactcaa tgttccggcg gcgaatagaa cgccggttat cgccatcaga
     2521 ataccggaaa ccgcataggt ggccgtgatc atgattttgc gtccgagcgt gtcgaacaaa
     2581 tgtcccaggg tcagcggtcc aagaaaattg ccgagtgcga aaggcaagat gaaccagccg
     2641 atgtctgaag ccggcacgcc gtagaaattg gtgaggacca gcgcataggt gaaaaagatg
     2701 gcgttgtagc agaaggcctg cgtcgccatc aggacgagtc cgagaatggt gcgtttggga
     2761 tagtgcctaa gaagggcacg aacgacatcg ccgaatcgcg tgccgcgata gggcgcaaaa
     2821 gacatgggca cgcaggcggt gcgcggcggc gcgatgcctt cgcgaacctc gatctcgccg
     2881 atcactcttt cggcttccgc aatgcgccca tgcgtcatga gccagcgggg actttccggt
     2941 acgaagtggc gcagccagac gataaaaacc cccagcactc ccccaacgat gaatgcgagc
     3001 cgccagccat aggcgatggg aagcaccgcc ggattgagca cgatcagcga agccagcgcg
     3061 ccaatggcgg cgccaaccca gaagctccca tttacgatga tatcggtgcg tccgcggtag
     3121 cgagccggta cgaactcctg gatcgccgaa ttgatggccg catattcgcc gccgatgccc
     3181 gcgccggtca aaacacggaa cagcgcaaaa gaccaaaaat cccacgcgaa ccctgtcgcg
     3241 acggtggcga cgacatagag gccgaccgta accgtgaaga gctttttgcg cccgaaccgg
     3301 tcggtcagat cgccgaacag cagcgcgccc agaaccgcgc cgataatata ggcgctgccg
     3361 gtcaaaccga tttgtgtgct ggtgagcgcg agtgtcgcgc cttgcgtcag cacacccgcg
     3421 agcgagccga ccagcgtcac ttccaggccg tccagaatcc aggtgatgcc cagcgcaacc
     3481 accaccagcc aatgaaagcg gccccatcga aggcggtcga gccgtgcggg tacgtcgctc
     3541 aggaaagtgt cgtcgccgag a
//
LOCUS       DASYEG010000101         7248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2494500, whole genome shotgun sequence.
ACCESSION   DASYEG010000101 DASYEG010000000
VERSION     DASYEG010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2494500"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VGM72_03815"
     CDS             <1..192
                     /locus_tag="VGM72_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264420.1"
                     /translation="QHPSRDPFRFWFDTVKSHMQGHMDGVPRLEFEMIFIFRDIANEG
                     LVRTNTIDRFLLRVGYHVL"
     gene            182..1768
                     /locus_tag="VGM72_03820"
     CDS             182..1768
                     /locus_tag="VGM72_03820"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein D"
                     /protein_id="HEY4264421.1"
                     /translation="MSYEIDRRSVLRAIAAYTAFGFVTGRDSEALAAQRGLVLGKPFA
                     FSFDALKEIARQAVNRPYERPNPASDIVSRIDYEAWGKIIFNTDYALFQGERFPVTFF
                     HLGKYFQKPVEMHAVDGGEARELIYDQRYFDMPADSIAHQLPKASGFAGFRIQEPRDG
                     KRDWRKNDWVAFLGGCYFRAIGELYQYGASARGVALDTVVAGKDEEFPDFTKIYIVKP
                     DESSDAVTVYSLLEGPSVVGAYKFVMAREKGVVMDIEARLFLRKPVSRFGIAPLTSMY
                     WFSETAKPTAIDWRPEVHDSDGLAMWTGWGEHLWRPLNDPPRIMASAFSDENPKGFGL
                     LQRDRAFDHYLDGVYYDRRPSLWVEPKGNWGKGAVQLIEIPTDDEIRDNIIATWVPET
                     PAAAGSEFNIAYKLYWLADEPHPSDLARCVATRLSDGGQPGQPRPKGVRKFLVEFLGG
                     PLSTLPFGVKPSMVMEASRGHFTDYQIMEAVPDGQAGHWRAQFDLAGVEGSDPVEMRL
                     HLTVGDKIATETWMFQYHPF"
     gene            complement(1796..2221)
                     /locus_tag="VGM72_03825"
     CDS             complement(1796..2221)
                     /locus_tag="VGM72_03825"
                     /inference="COORDINATES: protein motif:HMM:NF017936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY4264422.1"
                     /translation="MTSDEIIAALDLQPHPEGGFYRETFRDYAGPDGRARLTAIYYLL
                     RAGEVSRWHRIDAAEIWHWYDGDALELSFVGSGTISRVPLGPKVSEGQRPQIVVQAGA
                     WQCAKPLGSYVLCGCTVAPGFEFSKFEIAPEGWSPPSHF"
     gene            complement(2329..3255)
                     /locus_tag="VGM72_03830"
     CDS             complement(2329..3255)
                     /locus_tag="VGM72_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264423.1"
                     /translation="MMMLRRLLTAAALAAISAAPASGQASPGGAAVPDFSGVWAHPWL
                     PGFEALASGSTALVNLARTPEGRSIQTQLVGDYKNPILKPEAAETVRKLGEQSSAHFG
                     FHNPRNQCWPNGLPFLLSSNGQQIFDRGDHLTMIYRVDHQVRTVRMKSSHPANVRPSY
                     YGDSVGHYEGDTLVIDTVGIKPGPFAMIDWFGTPQTAALHVVERYRLIDRDRAQDGVR
                     RANKDNVLPQFNNPPVVDFNSKAKWLQLLFTVDDPNVFTTPWSATVTFGPAVQSVGAG
                     ANPWPEDVCAENPHKYGTEEDVRLPTARVADF"
     gene            complement(3382..4545)
                     /locus_tag="VGM72_03835"
     CDS             complement(3382..4545)
                     /locus_tag="VGM72_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEY4264424.1"
                     /translation="MERSHARILTTHVGSLIRPAELLDQLRTVQSGKGDVAAYEQCLK
                     ESVAQVVRRQADTGIDIVDDGEFGKNISWSQYALERLSGFERRPVPLEKNPFAAGADR
                     TRFKEFYAEMDAAQAPSSDWESVCAGPIAYTGTAALERDIADIKTALKGVAVTEAFLP
                     VAAPASVIPDRKNAYYKNDEEMFQALAGAMRTEYRTIAASGLLLQLDDARAAVTYDRM
                     VPPASFADYRKWLEHQIEVLNHAVEGIAPERIRYHVCWGSWPGPHTTDVPLKDIVDIL
                     MKANVGAFLIEGANPRHEHEWEVWEKVKLPDGKILIPGVISHATNIVEHPELIAQRIV
                     RYAKFLGRENVIAGTDCGFAQGPFHRRVHPSIMWAKFEALAEGARLATKQLWN"
     gene            4693..5559
                     /locus_tag="VGM72_03840"
     CDS             4693..5559
                     /locus_tag="VGM72_03840"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4264425.1"
                     /translation="MSVIDIHPHVISTDTARYPRAPLGGHQSDWSRERPIGVDRMIAA
                     MDQAGIAKSALVQASTCYGHDNSYVADAVAAHPERFTGVFSADVLAGDAVDRMKYWMA
                     KGLTGMRLFTTGSTMPGQAGWLDDPRSFPAWEFAQKSNLPVCLQMTAQGIAQLRGILE
                     RFPKLKVVLDHLARPVQEDGPPYHGADSLWGLAGFPGVYLKVTERNLVGAKSGKATPE
                     TFFTHLVSEFGASRIAWGSNYPASERPLPDLVKLAQETLSFLPAGDREWIFSGTAKAL
                     YPALAGKEGTGR"
     gene            5556..6452
                     /locus_tag="VGM72_03845"
     CDS             5556..6452
                     /locus_tag="VGM72_03845"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4264426.1"
                     /translation="MSEPVVLHCLLGNHPNTKALKSGSVSSPLVNFDYFEVKVANTQF
                     KAMVRELKYDFGELAMVTFLQAKHYGKPYVLLPATIVGRNQHHTIFYNAERGPLSPRD
                     LNGKRVGVRAYTQTTGAWVRGILAGDYGIDLNGVTWVTFEDSHLAEYRDPSHVERAGE
                     GKVLKQMLIDGEIDAAILGDVAEEGPLKRLIPDHESAGRDWARRHGGVPINHLAVMRE
                     SIVKARPDVVREVYRVLKESRAAADLPTGADDPLRFGIGAIRQSLEQLNAYAVAQKLI
                     PRAFSADELFADAARILGPEAD"
     gene            6726..6902
                     /locus_tag="VGM72_03850"
     CDS             6726..6902
                     /locus_tag="VGM72_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264427.1"
                     /translation="MKLEALTKGKSVRVSAISSSMSPEYKKAVYTLGNIRVSSGRRFR
                     MKLGVSALTITRTK"
BASE COUNT         1411 a         2166 c         2240 g         1431 t
ORIGIN      
        1 caacatcctt cgagagaccc ttttcggttt tggttcgaca ccgtaaagtc acatatgcaa
       61 gggcacatgg atggagttcc ccgcctggaa tttgaaatga ttttcatttt ccgggacatt
      121 gcaaacgaag gtttggttag aacaaacacc atcgaccgct tcctgttgag agttggctat
      181 catgtcctat gagattgacc gccgttcggt cctaagggct attgcggcct atacggcgtt
      241 tgggttcgtc acgggacgcg acagcgaagc tctggccgcg cagcgcggtc tcgttctcgg
      301 caagcctttc gctttttcgt tcgatgccct gaaagaaatc gcgcgtcagg cggtcaaccg
      361 gccctatgag cggccgaatc cggcttccga tatcgtgtct cggatcgatt acgaggcctg
      421 gggcaagatc attttcaata cggattacgc gctgttccag ggcgagcggt tcccggtcac
      481 cttcttccac ctcggaaaat atttccaaaa acccgtcgag atgcatgcgg tcgacggcgg
      541 cgaagcccgt gaacttattt acgaccaacg ctatttcgac atgccggcgg attcgatagc
      601 gcatcaattg ccgaaagcct cgggcttcgc cggcttccgc atccaagaac cgcgcgacgg
      661 caagcgcgat tggcgcaaga atgactgggt cgcgtttctt ggtggctgct acttccgggc
      721 gatcggcgaa ctctatcaat atggcgcatc ggcgcgcggc gttgcactgg ataccgtggt
      781 cgccggcaag gatgaggaat tccccgactt cacgaagata tacatcgtga agcccgacga
      841 aagcagcgat gccgtgacgg tctattcgct gctcgaaggt ccctcggtcg tcggtgccta
      901 caaattcgtc atggcgcgcg aaaagggcgt ggtcatggat atcgaggcga ggctgttcct
      961 gcgcaagccc gtgagccgct tcggcattgc gcctctgacc tctatgtatt ggttctcgga
     1021 aacggcaaag ccgaccgcca tcgactggcg gccggaggtt catgattccg acgggttggc
     1081 catgtggacg ggttggggcg agcatctctg gcggccgctc aacgatccgc cgcgcatcat
     1141 ggcgtccgcc ttcagcgatg agaacccaaa gggcttcggt ctcctgcagc gcgaccgcgc
     1201 attcgaccat tacctcgacg gcgtctatta cgaccggcgt ccaagcttgt gggtcgaacc
     1261 aaaaggcaat tggggaaagg gcgccgtcca actgatcgag attccgacgg acgacgagat
     1321 ccgcgacaat atcatcgcca cctgggtgcc cgaaacaccg gccgcggccg ggtccgaatt
     1381 caacatcgcc tacaaactct attggttggc cgacgaacct cacccgagcg acctcgcgcg
     1441 ttgcgtcgcg acgcggctca gcgacggcgg tcaacccggt cagccccgtc ccaaaggcgt
     1501 gcggaaattt ctggtcgaat tcttgggcgg tccgctttcg actttgccct tcggtgtgaa
     1561 gcccagcatg gtgatggagg cctcgcgcgg gcattttacc gactaccaga tcatggaggc
     1621 tgtaccggac gggcaggcgg ggcattggcg cgcgcaattc gatttagcgg gtgtggaagg
     1681 ctccgatccg gtcgagatgc gcctgcacct gacggtcggc gacaagatcg cgaccgagac
     1741 ctggatgttc cagtatcacc ccttttagcg cagcatcgaa ttgggtgatg gcgctttaaa
     1801 aatggctggg gggagaccag ccctcgggag cgatttcaaa tttcgaaaac tcgaacccgg
     1861 gcgcgaccgt gcagccgcac aagacgtacg acccgagagg tttggcgcat tgccaggcgc
     1921 cggcctgaac gacgatctgg gggcgttggc cttcgctcac cttgggaccc agcggtacgc
     1981 gactgatggt tcccgagccg acaaacgaca actccaaagc atcgccgtca taccaatgcc
     2041 agatttccgc cgcgtcgatg cgatgccagc gcgagacctc gcccgcgcgc agcaagtaat
     2101 aaatcgctgt caaacgcgcg cggccgtccg gacccgcgta atcgcggaag gtctcgcggt
     2161 aaaagccgcc ctcgggatgc ggctgcaagt cgagtgccgc gatgatctcg tctgacgtca
     2221 tgaaatcgtt ccttcaggga aaaagggatc ggatgctccg ggtggacgag cgcgatggat
     2281 cggaccgcga cgccgcggcc gtcggagagc acgtcgagct tgcgctcttc aaaaatccgc
     2341 gaccctcgcc gtcggcaggc ggacatcctc ttccgtgccg tatttgtggg gattttcggc
     2401 gcagacgtct tcaggccagg gattcgcgcc cgccccgacg gactgcaccg ccggaccgaa
     2461 ggtcaccgtc gcggaccacg gcgtggtgaa gacgttcgga tcgtccaccg taaagagaag
     2521 ttgcagccac ttggccttgg aattgaaatc gacgaccggc ggattgttga attgcggaag
     2581 cacattgtct ttgttcgccc gccgcacgcc gtcttgcgcc cgatcgcgat cgatcagccg
     2641 gtagcgttcc acgacatgca gcgccgcggt ctgcggcgta ccgaaccagt cgatcatcgc
     2701 gaacgggcca ggcttgatgc cgacggtatc gatcaccaaa gtgtcgcctt cataatgacc
     2761 aacggaatcg ccgtaataag acggcctgac gttggccggg tgcgacgatt tcatgcgcac
     2821 cgtgcgcacc tgatgatcca cccggtaaat catcgtcagg tgatcgccgc ggtcgaatat
     2881 ctgctgccca ttgctcgaca gcaggaaggg aagcccgttc ggccaacact ggttgcgcgg
     2941 attgtgaaag ccgaaatgcg ccgatgattg ctcgcccaat ttgcgcaccg tttcggccgc
     3001 ttcgggcttg aggatgggat tcttgtagtc gccgacgagt tgggtctgga tgctgcgccc
     3061 ttccggcgtg cgcgcgaggt tgaccagcgc cgtggaaccg gacgcaagcg cctcaaagcc
     3121 aggcagccag ggatgtgccc agacgcccga gaagtccggt acggcagcgc cgccggggga
     3181 tgcttgtcca gacgcgggcg ccgccgaaat tgccgccaat gccgctgccg tcaataaccg
     3241 cctgagcatc atcatcgcgc gcccccgcaa gtttgatgaa agcgatgcta caccggttcc
     3301 gggcccgtta aaactctccc aaccgcgacg ctcccacggg acgtatgcca gcgttccgcg
     3361 cggatcgatg cggacggtgc cttaattcca gagctgcttc gtcgccagcc gcgcgccttc
     3421 ggccagcgct tcgaacttcg cccacatgat ggagggatgc acccgccggt ggaacggccc
     3481 ctgcgcgaag ccgcaatcgg ttcctgcgat gacattctcg cggcccagga atttcgcgta
     3541 gcgcacgatg cgctgggcga tgagctcggg atgttcgacg atgttggtcg cgtggctgat
     3601 gacgccgggg atgaggattt ttccgtccgg aagtttcacc ttttcccata cctcccattc
     3661 gtgctcgtga cggggattgg cgccctcgat caggaatgcg ccgacattgg ccttcatcaa
     3721 aatgtcgacg atgtccttca agggcacatc ggtggtgtgc gggccgggcc agcttcccca
     3781 gcagacgtga tagcggatgc gttcgggagc aatgccttcc accgcatggt tcagcacctc
     3841 gatctgatgc tccagccatt tgcggtaatc ggcgaaactc gccggcggca ccatgcggtc
     3901 ataggtgacg gcggcgcgcg catcgtccaa ttgcagcagc agaccggatg ccgcgatggt
     3961 gcgatattcg gtgcgcatcg caccggcgag cgcctggaac atctcttcgt cgtttttata
     4021 atatgcgttc ttgcggtcgg ggatgacgct tgcgggtgcc gccaccggca gaaacgcctc
     4081 cgtcactgcg acgcccttca acgccgtctt gatgtcggcg atgtcgcgtt cgagtgccgc
     4141 ggtccccgta taggcgatgg ggccggcgca aaccgactcc cagtccgacg acggggcctg
     4201 ggccgcgtcc atctcggcgt agaattcctt gaagcgcgtg cggtccgcgc cggcggcgaa
     4261 cggatttttc tccagcggca ccgggcggcg ctcgaatccg gaaagccgct ccagcgcata
     4321 ttgcgaccag gagatgttct tgccgaattc tccatcgtcg acgatgtcga ttccggtatc
     4381 ggcctggcgg cgcaccacct gggccacgct ctccttcagg cattgctcat aggcggccac
     4441 atcgcccttg ccgctctgaa cagtgcgcaa ctgatccaga agttccgcag gcctgatcag
     4501 gctgccgaca tgggtggtga ggatgcgtgc gtggcttcgt tccatggacg tttcctgagc
     4561 attacaggac ctacgactag aagaaattcg cccggcccac aatcgagacc agaaaacaaa
     4621 atttgtggct atttgaccat cgcgcgcagc gatattttcg actaaaaggg ccgcgccttc
     4681 gggagcccgc ttatgtccgt catcgatatc catccccacg tcatttcgac cgatacggcg
     4741 cgttatccgc gcgcgcccct gggtgggcat caatcggatt ggtcgcgcga acgcccaatc
     4801 ggcgtggacc ggatgatcgc tgccatggac caggcgggca tcgccaaatc ggcgctggtc
     4861 caggcctcca cctgttacgg gcatgacaat tcctatgtcg ccgacgcggt ggccgcccat
     4921 ccggagcgtt tcacgggcgt gttctccgcc gatgtgctgg ccggcgatgc ggtggaccgg
     4981 atgaaatatt ggatggcgaa gggcctgaca ggcatgcggc tgttcaccac ggggagcacc
     5041 atgccggggc aggccggttg gctggacgat ccacgctcct ttcccgcctg ggagttcgcg
     5101 caaaaatcga acctgccggt gtgtctgcag atgacggccc aaggcattgc gcaattgcgc
     5161 gggattctgg aacgctttcc aaagcttaaa gtggtcctcg accatctggc gcgtccggtt
     5221 caggaagacg gcccccccta tcatggcgcg gacagcctgt ggggactcgc aggctttccc
     5281 ggggtgtatc tgaaagtgac ggagcgcaat ctcgtcggcg ccaaaagtgg caaggcgacg
     5341 ccggagactt tcttcacaca cctggtgtcg gaattcggtg cttcgcggat tgcctggggt
     5401 tcgaactacc cggcgtcgga gcggccgctg cccgacctgg tgaagctcgc gcaagagacg
     5461 ctcagctttc tgcctgcggg cgaccgcgaa tggattttct ccggcaccgc caaggcgctt
     5521 tatcccgcgc tcgccggcaa agaagggacc ggacgttgag cgaacctgtc gtactgcatt
     5581 gcctcctcgg caatcatccg aacaccaagg cgctgaaaag cggaagcgtg tcttcgccgc
     5641 tggtgaattt cgattatttc gaggtgaagg tcgccaacac gcaattcaag gcgatggtgc
     5701 gcgagttgaa atatgatttc ggcgagctgg cgatggtcac gttcctgcag gccaagcatt
     5761 acggcaaacc ctatgtgctt ctgcccgcca ccattgtggg gcgcaatcag catcacacga
     5821 ttttctacaa tgcggagcgc ggtcccctca gcccgcgcga cctgaacggc aagcgcgtcg
     5881 gcgtgcgcgc ctatacgcaa acgacgggtg cgtgggtgcg cggcattctc gcgggcgatt
     5941 acggcatcga tctgaacggc gtgacctggg tgacgttcga ggattcgcat ctggcggaat
     6001 atcgcgatcc ttcccatgtc gagcgggcgg gcgagggcaa ggtgctcaag cagatgctca
     6061 tcgacggcga aatcgacgcg gcgatcctcg gcgatgtcgc ggaagaaggg ccgctgaagc
     6121 gtctgatccc cgatcacgaa tccgcgggcc gcgactgggc gcgccggcat ggcggggtgc
     6181 cgatcaatca ccttgcggtg atgcgcgaat ccatcgtcaa ggcgcggcca gacgtggtgc
     6241 gcgaggtcta tcgcgtgctg aaggaaagcc gcgcggcggc ggatttgccc accggcgcgg
     6301 acgatccgtt gcgtttcggg atcggcgcca tccgccaatc gctggaacag ctcaacgcct
     6361 atgcggtggc gcagaagctg atcccacgcg ccttttccgc tgacgaattg ttcgccgatg
     6421 ccgcccgcat cctcggcccg gaagcggatt gatttgtcac cccgaagcag agttctcata
     6481 aggttaaacg ccgcggtttc cggcgatagg cgggaacgcc ggtcttgcct taaaagcaga
     6541 catttattga agtcgaagaa gaggacgcag ctcgctaacg tgcggccatg attgtgcact
     6601 tccggcgtgt gagtccatta tcggacagcc ggagcgggtg gaatgttgca ggagagctac
     6661 gacccgtgca aagggcgtat cagcaagagg tggaaacagg aatgacggca aaaagcagac
     6721 tacgaatgaa gctcgaagca ttgacgaagg gcaaaagcgt gcgcgtgtca gccattagct
     6781 cgagcatgag ccccgaatac aaaaaagccg tgtacactct cggcaatatt cgagtcagca
     6841 gcggccggcg cttccgtatg aaactcgggg tgagcgctct gaccattacc cgcacgaaat
     6901 agacgataga tttgcatcgc gcggaatcag ccgatgagcg cgtgtcaccg gaacgaccct
     6961 agtcgtggcc tctaacatct gcggtgtgcg gtatcctggc cgtcggcaac acagctttgt
     7021 gtgcggctcg aatcgaccat ctgggatacc cattttaatt tggagccgat cttgaacaga
     7081 gcaaaaatgc gcccggagcg aaccctcccc gatacgccag cgctggtgcg aagtctcgtt
     7141 cattaccgag aaccaagtca cgcgcgtgct ttgtttgaac tcggtgtcac cgccggggcg
     7201 cttgcgctgt tttgggtctt gatgtggctg tcgcttggcg ttggctat
//
LOCUS       DASYEG010000102         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4234993, whole genome shotgun sequence.
ACCESSION   DASYEG010000102 DASYEG010000000
VERSION     DASYEG010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4234993"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VGM72_03855"
     CDS             <1..592
                     /locus_tag="VGM72_03855"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY4264428.1"
                     /translation="MAPHFVLEDSGQKYVARKVDCGGGEQYTEEYKKNVNPHSRVPVL
                     QLDNGAWISENIAILPYLGKRFGFWTEDPVLEAKMLSFIGWVASTVHPAQAHYRRAYR
                     YASDEAVHPNMQKVGLESFHTYLKDVDKMYAGKAYLFGDKLSVCDHYAFVLYTWGLQR
                     GFAMADEMPHFTAFNVEMRKRPAIQHVFEDENIDVR"
     gene            676..>1502
                     /locus_tag="VGM72_03860"
     CDS             676..>1502
                     /locus_tag="VGM72_03860"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4264429.1"
                     /translation="MGQPFRIDVHHHPTPTAAIANGRRAVPPNMKDWTAAMSLEDMDK
                     NDVQTSILSLNHPIIVWPSDQAAAIKQAREWNEFMVKQAKDHPGRFGVFAVLPILNVE
                     ASLKEMEYALDTLKVDGINMMTNIGDKWLGDPHYWPLFEELNRRKAVLYTHPMAPTCS
                     VNAMMPEVNDSVIEFATDTSRAIARILFSGAAHKFKDINFIFSHAGGTMPYILERYTR
                     HPLTDKELAARVPDGVVNYLKRWHYDTAQASHPFAMSSITKLVNVSQLLFGTDFPYR"
BASE COUNT          321 a          470 c          428 g          283 t
ORIGIN      
        1 gatggcgccg catttcgtgc tcgaggacag cggccagaaa tacgtcgccc gcaaagtcga
       61 ttgcggcggc ggcgagcaat acacggaaga gtacaagaag aacgtcaatc cgcattcgcg
      121 cgtgccggtg ctgcagctcg ataacggcgc gtggatttcc gagaacatcg ccatcctgcc
      181 ctatctcggc aaacgcttcg gcttttggac cgaggatccg gttctcgaag cgaagatgct
      241 gtcgttcatc ggttgggtcg cgtcgaccgt gcatccggcg caagcgcatt accgccgcgc
      301 ctatcgctat gcgagcgatg aagcggtaca tccgaacatg cagaaagtcg gcctcgaatc
      361 cttccacact tatctgaagg acgtcgacaa gatgtatgcc ggcaaggcct atcttttcgg
      421 cgacaagctg tcggtctgcg atcactacgc cttcgtgctc tacacgtggg ggctgcaacg
      481 cggtttcgcg atggcggacg agatgccgca tttcaccgcg ttcaatgtcg agatgcgcaa
      541 gcgcccggcg atccagcacg ttttcgaaga cgaaaacatc gacgtccgtt gacacgcgtg
      601 cggcatggcg cggccgccgg atagcgttaa agcgcgcggc gacctatagt cgcgcgcagc
      661 cattttggag gaatcatggg ccagccattc cgtatcgacg tgcaccatca tccgacgcca
      721 accgccgcca tcgccaatgg ccgtcgcgcg gtgccgccga atatgaaaga ctggaccgcc
      781 gccatgtcgc tcgaagacat ggacaagaat gacgtccaga cgtcgatcct ctcgctcaac
      841 catccgatca tcgtgtggcc gagcgatcag gctgccgcga tcaagcaggc gcgcgagtgg
      901 aacgagttca tggtgaagca ggccaaggac catcctggcc gcttcggcgt cttcgccgtg
      961 ctgccgatcc tcaatgtcga agccagcctc aaagagatgg aatatgcgct cgacacgctg
     1021 aaggtcgacg gcatcaacat gatgaccaat atcggcgaca agtggctcgg cgatccacac
     1081 tattggccgc tcttcgaaga actcaaccgg cgcaaggcgg tgctctacac ccatccgatg
     1141 gcgccgacct gctcggtcaa tgccatgatg cctgaggtga acgattcggt gatcgaattc
     1201 gccaccgaca cctcgcgcgc catcgcccgc atcctgttca gcggcgcggc gcataaattc
     1261 aaggacatca atttcatctt ctcgcatgcc ggcgggacga tgccctatat cctcgagcgc
     1321 tatacgcgcc acccgctgac cgacaaagaa ctcgccgcgc gcgtgcccga cggcgtggtg
     1381 aattatctga agcgctggca ctacgacacg gcgcaggcct cgcatccttt cgcaatgtcg
     1441 tcgatcacca agttggtcaa tgtctcgcaa ttgctgttcg ggacggactt cccctaccgc
     1501 aa
//
LOCUS       DASYEG010000103         3013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2929784, whole genome shotgun sequence.
ACCESSION   DASYEG010000103 DASYEG010000000
VERSION     DASYEG010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2929784"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="VGM72_03865"
     CDS             <1..1108
                     /locus_tag="VGM72_03865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HEY4264430.1"
                     /translation="QVTSSQNLTQQGLLTSTGSSTDLAISGNGFFVVTDNPGNPQAAL
                     YTRAGSFVPDAQGRLKNSAGFYLEGWTLQPDGSMPANRSSLGLINLSSLNGTAEATTT
                     MSLRANLQASAAIVSPYTAGDMTAGTVTPEFEQTINVYDSQGAARPMQLAFVKTGADT
                     WKYEVSYQGPVADIGGAGNNPVATGTVNFNADGTLSIPPTATLNIPWAASTGLNPQTV
                     TITLGTSGSSNGVTQFDAPSSLTSATVDGAPYGALTSVSVDDQGFVTALFDNGIEKKV
                     FKVPVATFTDPDALAPVAGNAYQLTDASGTAIIQEAKNGGAGSIASNSVEASTVDLAK
                     EFSDLITTQRAYSAATRIITTADQMLQELMQIKQ"
     gene            1271..1549
                     /locus_tag="VGM72_03870"
     CDS             1271..1549
                     /locus_tag="VGM72_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HEY4264431.1"
                     /translation="MAEEQRRSRVSYVIGPDGSPLTLADLPPPGTKRWVIRRKAEVVV
                     AVRGGLLTLDEACTRYTLTVEEFLAWQRAIERFGLAGLRATRVQQYRH"
     gene            1782..>3013
                     /gene="fliF"
                     /locus_tag="VGM72_03875"
     CDS             1782..>3013
                     /gene="fliF"
                     /locus_tag="VGM72_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEY4264432.1"
                     /translation="MPEFFKGIGPARIGAMAGVAAALTAFFLYVFGMIAEAPKAILFS
                     GLEAREATSVITKLEALNIPYEQKGDGGTILIPSDQVSKIRMQLASEGLPSAGVGYEI
                     FDKTDTFGTTAFVQNVNRLRALEGELARTIKSLDSIDSARVHLVIPERQVFARDNQPP
                     SASVVVKTRSRLSRGQVSAIQHLVAAAVAGLTANNVALVDDKGELLAGGGGADGDAAT
                     GAVQEERTSGFEDRLRQRVENIVSSIVGPGHSRVQVAAEMDFNRVTQTAETFDPDSRV
                     VRSSQTVQQDSQSKDNHGGAAPVSVGTALPGQAAPATPPNDGSTSANTRTEETVNYEI
                     SKVTKTQVLDAGQVKKLSVAVVVDGTYTPGANGQRAYAPRSTDDMGKITALVRSAIGF
                     DEKRGDQLQVTNMRFADI"
BASE COUNT          619 a          940 c          909 g          545 t
ORIGIN      
        1 gcaggtcaca tcctcacaga atttgacgca gcaggggctg ctgacctcga cgggatcctc
       61 caccgatctc gcgatttcgg ggaacgggtt cttcgtcgtc acggacaatc cggggaatcc
      121 gcaggccgct ctctataccc gcgcgggctc ctttgtgcct gacgcgcagg gccggctgaa
      181 gaactcggca ggcttctatc tcgaaggctg gacgctgcag cccgacggca gcatgccggc
      241 gaaccgcagc tcgctcgggc tgatcaatct gtccagcctg aacggcacgg ccgaagcgac
      301 gacgacgatg tcgttgcgcg ccaatctgca agcctccgcg gctatcgtca gcccgtacac
      361 cgccggcgac atgacagcgg gcacggtgac accggaattc gagcagacca tcaacgtcta
      421 tgacagccaa ggcgccgcgc gccccatgca gcttgcgttc gtgaaaacgg gagccgacac
      481 atggaaatat gaagtctcct accagggtcc ggttgccgat atcggtggcg cgggcaacaa
      541 ccccgtcgcc accggcaccg tcaatttcaa tgccgacggc acgctgtcga tcccgccgac
      601 ggcgaccctc aacatcccgt gggcggcctc caccggtctc aacccgcaga ccgtgacgat
      661 aaccctcggc acttcggggt cttccaatgg cgtgacgcag ttcgatgcac catcctccct
      721 gacctcggca accgtcgacg gcgcgcccta tggcgcactg acttcggtgt cggtggacga
      781 tcaaggattc gtgaccgccc tgttcgacaa tggcatcgag aaaaaagtgt tcaaggttcc
      841 ggttgcgaca ttcaccgatc cggacgcgct ggcgccggtt gccggcaacg cctatcagct
      901 gaccgacgca tccggcacgg ccatcatcca ggaagccaag aacggcggcg ccggctccat
      961 tgcctccaac tcggtggaag cttcgaccgt cgatctggcg aaggaatttt ccgatctgat
     1021 cacgacgcaa agagcgtatt cggcggccac gcgtattatc accaccgccg atcagatgtt
     1081 gcaggaactg atgcaaatca aacaataagg tatgaaacgg tgcccgccgg acggtaaagg
     1141 caccgtttca acaaacaatg ctaacgaaat cattgggtta ttccttcttt taccctgact
     1201 tttggggctt tcttaaaagc ggcagcggta atttggggtg tgtgggaaaa gaaggtggag
     1261 catgaggaat atggctgagg agcaacgccg gtcgcgcgtc agttacgtga tcggtcccga
     1321 tgggagtccg ctcactctgg ctgatttgcc gccgcctggc accaaacgct gggtgatccg
     1381 gcgcaaagcc gaagtcgtgg tggcggtgag gggcggactg ttgaccctcg acgaggcctg
     1441 cacgcgctat acgctcacgg tcgaggaatt cctggcctgg cagcgcgcga tcgaacgctt
     1501 tggattggcg ggcttgcgcg caacacgcgt gcagcaatat cggcactagc gaaacgagag
     1561 ccggaacagc acgccttcca tcgtcattct ccagataggg ttaaagaatc attttcacga
     1621 tgcattgcat tttcaatgtt gcgatatcgc gaaaattcgt aatcccgtta agctgaattt
     1681 tacccagtcc cggcaatgtt tgcctagttc agcggcgagc cgaaagagag cgacaacgcc
     1741 tctcgcatcc ggctcgatcc gcgttctagg cggaggttag gttgccggaa tttttcaaag
     1801 ggatcggacc tgcgcgtatc ggcgccatgg cgggcgtcgc tgcggcgctg acggcattct
     1861 tcctgtacgt attcggcatg atcgccgaag cgccgaaggc gatcctgttc tccgggctcg
     1921 aagcgcgcga agcgacttcg gtcatcacca aactggaagc cttgaacatc ccctatgagc
     1981 agaagggcga tggcggcacc atccttattc cgtccgacca agtctcgaag atccgcatgc
     2041 agcttgcgtc ggaaggcctg ccgtccgccg gcgtcggcta tgagattttc gacaagaccg
     2101 acacgttcgg cacgacggcc ttcgtacaga acgtgaatcg cctgcgcgcg cttgagggcg
     2161 agctggcccg caccataaaa tccctggatt cgatcgactc ggcgcgcgtg cacctggtca
     2221 ttcccgagcg tcaggtcttc gcccgcgaca atcagccgcc atcggcctcg gtcgtggtga
     2281 agacgcgttc gcgcctgtcg cgcggccaag tgagcgcgat tcagcatctg gtggccgccg
     2341 cggtggccgg acttacggcc aataatgtcg ctctggtgga tgacaagggc gaattgctcg
     2401 caggcggcgg tggcgcggac ggcgacgccg cgacgggcgc ggtccaggaa gaacgcacgt
     2461 cgggtttcga agatcgtctg cgccagcgcg tcgaaaacat cgtctcctcg atcgtgggac
     2521 cggggcattc ccgcgtccag gtcgccgccg agatggattt caaccgcgtc acccagaccg
     2581 ccgaaacctt tgatccggat agccgcgtgg tgcgttcgtc ccagacggtg cagcaggatt
     2641 cgcagtccaa ggacaatcac ggcggagcgg cccccgtcag cgtcggcaca gcgcttccgg
     2701 gacaggccgc cccggcaacc ccgccgaatg acggctcgac atccgccaac acgcgcaccg
     2761 aggaaaccgt caattacgag atctccaagg tcacgaagac tcaggtgctc gatgcgggac
     2821 aggtaaagaa gctttccgtc gccgtggtgg tggacggcac ctatacgccg ggcgccaacg
     2881 gacagcgcgc ctatgcgccg cgcagcaccg acgacatggg caagatcacc gcactcgtac
     2941 gctcggccat cggcttcgac gaaaaacgcg gcgaccagct ccaggtcacg aacatgcgct
     3001 ttgcggacat cga
//
LOCUS       DASYEG010000104        33458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1770664, whole genome shotgun sequence.
ACCESSION   DASYEG010000104 DASYEG010000000
VERSION     DASYEG010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 33458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33458
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1770664"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..141
                     /locus_tag="VGM72_03880"
     tRNA            67..141
                     /locus_tag="VGM72_03880"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:99..101,aa:Gly,seq:gcc)
     gene            799..1404
                     /locus_tag="VGM72_03885"
     CDS             799..1404
                     /locus_tag="VGM72_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEY4264433.1"
                     /translation="MKTVIIQTATALVLTTFFAVSADAQNQGSAGPPVLASKLAPARS
                     GSTQLLVTSSAFGSGSELDDKFTQNGDNMSPPLAWTKGPPGTQYYAVLAEDAGVDRAE
                     PIVHWVIYNIPSSMRGLPQNVPTDATLENGAVQGKNVRGAAGFIGPKPPKGQTHPYHF
                     EVFALNAKLNLPANADRNAVVGAMRNHVLAQGDLMAMYTGK"
     gene            1555..2613
                     /locus_tag="VGM72_03890"
     CDS             1555..2613
                     /locus_tag="VGM72_03890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY4264434.1"
                     /translation="MSTRMFKFLQSLSPPVLLTTLTGLTLVIGYFDYRAGTDTTFTAI
                     YLFPIGAAAWFFDRRTASVLAAVSSILSVSGDMEAGAHYASLWIPLWNIAARFAVFVF
                     AAHLIGALRKLNADLEKRAAERAAKLTEEIAMRERLQRELLQISEREQERVGHDIHDS
                     LCQHLTGTALAAQVLAESLQSRNPAEQDNAVRVVELVEEGIVLARNLARGLNAVEVSN
                     NGLMAALTDFAASTSDLFNVSCRFECSEPFLMDDPTAAVHLYRIAQEAVGNAIKHGEA
                     KRIVIRLERSNAGRVLRVIDDGTGVRASRLDGKAKGMGLRIMSYRSELIGGRIHIQRR
                     TPAGTEVTCSLPLEEAAQ"
     gene            2610..3272
                     /locus_tag="VGM72_03895"
     CDS             2610..3272
                     /locus_tag="VGM72_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4264435.1"
                     /translation="MTSAKARIVIVDDHPLVREWLTALIEKHPDLTVCGEADNAATAL
                     RIISEDPPDLAIVDLSLGSKSGIELIQSIRSSHPGVAMIVLSMYDEQVYAEHCIRAGA
                     RGYIMKRETAKNIIDAIHEVLAGNLYLSKRMTELMVQKAVSADASNGGVPLAQLSERE
                     LQVFKLLGQSREIDEIAKMLDVNIKTVHTYCARIKEKIKVSTTAELLHEARLWQGALP
                     PA"
     gene            complement(3299..3688)
                     /locus_tag="VGM72_03900"
     CDS             complement(3299..3688)
                     /locus_tag="VGM72_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264436.1"
                     /translation="MRDFALYAALGAAVFIAPAMAQEAGTAGQNPSQESAIRAGHEIA
                     LESCTGCHVASPQQEFRPVTGDAIPSFEDIANRPGVSVVSLREALDRTHRNKDAPQRT
                     SISTTTSISDVEASEVIAYILTQKRAP"
     gene            4003..7239
                     /locus_tag="VGM72_03905"
     CDS             4003..7239
                     /locus_tag="VGM72_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY4264437.1"
                     /translation="MYAIVRLALRYRYTAVVIAIFISIFGISAAVTTPADIFPDIKIP
                     VIAVVWTYRGLSPNDMAGRVIYYYERQLSTYVNDIEHMESQSLFGIGIVKIYFHPGAD
                     IRTATAEVDSVSQTVLKQLPPGITPPLILNYNASTVPILQLALSSATLPEQEIYDLGQ
                     NSIRPRLATVQGTAVPSPYGGKERQIQIDLDPQALQAKGLSAQDVETAIAAQNQIIPA
                     GTTKIGQFEYNVKLNDSPVTVAELNDLPIRSQDGSTTYIRDIGHVRDGFPPQRNIVRV
                     DGRRAALMTILKSGSVSTLAIVDGVKSLLPRLEETLPKSLRIAAIGDQSLFVKAAVSD
                     VIREGVIAAALTSLMILVFLGSWRSTLIIATSIPLAILASIAALSALGQTLNIMTLGG
                     LALAVGILVDDATVTIESINWHLERGKPIQAAIFDGARQIVAPAFVSLLCICIAFVPM
                     FFFPGVSGFLFAPMAEAVVFAMIASFVLSRTLVPVMANHLLVAHGGAHAVAVMRSHDS
                     ETGIRTTRNPFILFQHGFARRFDRARDGYRNLLELALGNRRIFIAGFMLAVLASFALI
                     PFLGQDFFPSVDSGQISLHVRAPVGTRIEETAALFDRIENNIRSVIPPKQLGTIVDNI
                     GLPVSGLNRAYLNTGGIGPEDGDILVSLTPGHAATAGYVKTLRGLLPRSFPGSGFAFL
                     PADIVSQILNFGAPAPIDVTVSGPNKTANETYAQELLRGIAKIPGTADARIEQSSHYP
                     ELAIDIDRTRANQMGVTERDVTNSLVVNLASSFQVAPAFWLDPSNGASYPIVAQTPQY
                     RLDSLSKMENLPVTGSKPGNMQVLGALGTIHREDSDAIVSHYAIQPTIDIYAATQGRD
                     LGAVAADIQQLIQQTGKDRPKGATVTMRGQVETMNSAFSGLFIGLAEAVILIYLLIVV
                     NFQSWADPFVIVSALPSALAGIVWMLFATDTTLSVPALTGAIMCMGVATANSVLVVSF
                     AREQLELTGDAVGAALEAGYTRFRPVLMTALAMIIGMAPMALGLGEGGEQNAPLGRAV
                     IGGLIVATAATLLFVPVAFSLVHGRTKSQPLPSDAASLPERDVYAPAE"
     gene            7223..8413
                     /locus_tag="VGM72_03910"
     CDS             7223..8413
                     /locus_tag="VGM72_03910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY4264438.1"
                     /translation="MPQQNDMEKPRRRTIRRLKQTAAVGVGVAAIIVASGIWVRLHAS
                     SEQASWTTEQTVPTVNVVHPSGLGGNGVLTLPGNLQAFYTARIYARVPGYVRQWYKDI
                     GAVVHRGDILADIDTPELDQQIEQVKADLANAQAAQKLAETTAARWANLLAVDAVSKQ
                     EAEEKAGDLAVKNAQVNAAKANVDRLESMKTFARITAPFDGVVTERATDVGALVNAGA
                     GSTGSPLFTVSDLHKIRVYVQVPQNYSAQIHNHMKAELSLPEYPGETFSAQLVSTSDA
                     ISDQSNTLLVQLEADNRMGKLKPGEYAQVSLLLPADKGMLRIPSSALVFRGEGLQVAT
                     VGQDNHVVMKPIRIANDLGPQVEVSSGLDASDKVIDNPPDSLAMGDAVRVAGPQDQQH
                     ADAK"
     gene            8415..9857
                     /locus_tag="VGM72_03915"
     CDS             8415..9857
                     /locus_tag="VGM72_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY4264439.1"
                     /translation="MRAAGLVSALLGASWLAGCSLAPRYEVPASTIPVSFKENGMWTQ
                     ATPQDAIPRGNWWSVYRDPTLAALEARIEGSNPTLAQALARYDEASAYLAEAQASLFP
                     LVGSNAQISQNRQSDERPLRGSNQPNDYANNSLTASISYDLDFWGRIRNIVQAGKAEQ
                     QAQAADLESVRLSLEAQLADDYVKLRAMDAELDLYRVTLAAYERAFNLIDQRYTQGAA
                     SGLDLGRAQTQLESARAEISNVAAQRALYEHAIASLVGTPASAFSLTPEIVTLNVPNV
                     PTGLPSTLLQRRPDIAAAERRVAEANAQIGVARAAFFPSIILAATAGFQDSGGVGVGL
                     VSAPNLLWSVGPAAALTLFDAGARQARVDATVARRNQTADIYRTAVLQAFQDVEDNLA
                     VLNNLAAAATAEDAAVGAAARTETLSMDRYDLGAASYLDVVTAQTAALQARLSALDFH
                     TRRLESSVRLIRALGGGWPASEAYDVTALP"
     gene            complement(9921..11405)
                     /locus_tag="VGM72_03920"
     CDS             complement(9921..11405)
                     /locus_tag="VGM72_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264440.1"
                     /translation="MNFKSILAGTITCVALAGPAFGQAATAPAAPVDKKIHFKGITLS
                     LGGFLAAETVWRSNNLESDIGSPGFAKIPFAGPGPGGTGNNVGVGHTGEFRMTARQSR
                     VQGLAEGDFDPNTHFAMYGEFDFMGGPASANSNESNSYAPRIRNLYGSADWSDLGIHV
                     LFGQSWSLATLNGQGMSERTELAPPTIEAQYSAGFVWTRQAQLRVVKNFGNEFWVGLS
                     IENPQTTIGGTAPAGYTGLTVSSGSGFNGGSTNVGLSEFNPGITLSLNHVPDLIGKVA
                     WEPSFFGRNVHLEAVGMYRDFYDRFGTSLTDVSNHNVSGGGGGVAGLIKLVPGTLDVQ
                     FDTLFGEGIGRYGSSQLPDVTYNANGTFAPLRQDIEMIGLTWHATRALDIYVFAGREH
                     DDSDFFSGTLNGTSQNLGYGNPAFNNTGCSSFSSTASCTGNIQTVEQINVGLWDNTYD
                     GDFGRVRIGVQYSYTYLKAFNGVGGSPHTSDNMVFTSFRYYPFQ"
     gene            11693..12727
                     /gene="pstS"
                     /locus_tag="VGM72_03925"
     CDS             11693..12727
                     /gene="pstS"
                     /locus_tag="VGM72_03925"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF008171.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEY4264441.1"
                     /translation="MRLYFSFPASILAGVIAVGLATDVSAADISGAGSTFAFPIYGKW
                     AAAYQMVSGSRLNYQSIGSGGGIKQIESKTVTFGATDMPLPTKELDQYGLIQFPTVIG
                     GVVPVVNIAGVKSGDVVLDGPTLADIYLGKIKTWNAAAIKKLNPTVNLPNQAIVVIHR
                     SDASGTVYIFTNYLSKVSAEWKDHVGNATGVDWPAGIGAKGSEGVAGNVMQSNGAIGF
                     VEYAYAKQNNLSYTRMLNHDGKAVSPVAAAFAAAASGAAWNPADGFATMLTDQAGQGS
                     WPISGATWVLFYKNPPDKAATAEGLKFFQWSFKNGDQIAAGLDYVPFPTAVKDKIESS
                     WTQVQGWSAR"
     gene            complement(12809..13396)
                     /locus_tag="VGM72_03930"
     CDS             complement(12809..13396)
                     /locus_tag="VGM72_03930"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEY4264442.1"
                     /translation="MSPIEMARGLCPRSLVTFFRRFHSAREGVIATEFALIAPIMIVM
                     YFGVAEIADAFTASSKVTSVASSAADLTARESAVCNAEISDIFSAMIAIMFPYPQNNM
                     QITISSLIDTGTGTVKVAWSDALNTTPRLVNSVVAIPAGLVTSGGGGSVIFTEVTYNY
                     SSPAGYLIYGTRPLHDSFYLRPRRTAQITRSANGC"
     gene            complement(13383..13928)
                     /locus_tag="VGM72_03935"
     CDS             complement(13383..13928)
                     /locus_tag="VGM72_03935"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEY4264443.1"
                     /translation="MRSVCSIFRRRENSGAAAAEFAMIAPVFMLLTFAILETSMVFFA
                     NMILDNAVIDTGRLIRTGQAQTGSMTQADFRNAVCDQVKILLSCDPTNLLIDVRSFAN
                     FSGSAFPPALDAAGNLNPNLNAYQPGGSSDATGSSIVLVRVLYKWQLYTPLFAQYFEN
                     MGKGSGVRLIAFSAAFKNEPY"
     gene            14269..15726
                     /locus_tag="VGM72_03940"
     CDS             14269..15726
                     /locus_tag="VGM72_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEY4264444.1"
                     /translation="MIFALSSLTLFSAAGAGIDMSRAMNAKSRLAGALDAAALAVGTT
                     NGLTTPQLQTLAQKYFDANYPATALGTHTPVTVAVNGQSITLGVTGRVPTTLLQVAGI
                     GDVDLSVTNQVTKAVTKLRVALVLDNTGSMSQTDNTGTSKISALKTATHQLLTQLQGA
                     AVNPGDVQVSIVPFSLDVNYGTSNPAATWIDWTDFDAQSRQPSYSVGPGSTCPYTNGT
                     DGFKCAKSPVNDPNCNVGSNNTCVSTVPSSGTYKGYICPSAHQTDASNGRGGHFYNGC
                     WDSVLVSGRYTHTWHGNTHTTWTGCFMDRAQNYDTNNSTPVVATPATLFPAENNLYCP
                     PVALNALSYDWASLGSKVDAMTPNGSTNQTIGLAWGFQALTSTNPLNAPAQDPTIQNV
                     IVLLSDGLNTQNRFAGDGSNQSSAVDARMALACTNAKAAGVRIYTVLVMSGNSSILQN
                     CATDSKKYFSLNTAGQIVTAFNTIGTELANLHLSR"
     gene            complement(15795..17552)
                     /gene="malQ"
                     /locus_tag="VGM72_03945"
     CDS             complement(15795..17552)
                     /gene="malQ"
                     /locus_tag="VGM72_03945"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEY4264445.1"
                     /translation="MDSIEERAACWGIETQYWDGLGQHRIVTEDVLSRLIEILGLNGK
                     PTALDFPAVSVSHPAYQGHGNAPERMWGLAVQLYGVRSRHNWGHGDFTDLEMLIEIAA
                     GLGASAIGLNPLHALFDNLADSYSPYSPSSRLFLNTHYIDAERIEEFPGLDEAGLADD
                     VSALRQSELVDYVGLIKTKTRAFSLAYANFRRHGTARRKREFASFRERGGHALLRFSC
                     FEFLRRQHDGPWWTWPAEWRTPEDERLAALRASQDEATGIHEFLQWVADSQWRACSTQ
                     AAALGLPIGLYLDIAVGVRSDGFDAWNQQSVFEPALEIGAPPDRLNLEGQRWGLAGIN
                     PIGLAEQGCAAFRRMLAASMQYAGSIRLDHVLGLKRLYLIPKDMRADQGAYVRLPFEP
                     LLAAVSQESLAHRCVVIGEDLGTVPDGFRETLADWGLWSYQVMLFQRAADGGFVAPDH
                     YARNALVTFATHDLPTFAGWMSSRDLAVKRALGMDPGESDQERAAARDAIARTMASRG
                     FHTLDYPSLDRFLSETPSRLLMVSAEDVLGMMDQVNVPGTIAEHPNWRRRLPVCLEDL
                     KGDRRLIALAKILSAERGR"
     gene            17623..19779
                     /gene="glgB"
                     /locus_tag="VGM72_03950"
     CDS             17623..19779
                     /gene="glgB"
                     /locus_tag="VGM72_03950"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEY4264446.1"
                     /translation="MQTEPLPPAAYAIVEGRHHDPFHYLGPHVEDGVCFVRVFLPGAS
                     QVAVILKNGREFSLARLHDAGLFGAPISGGDSHYRLRAVFDGKTVEMEDAYRFKRVLS
                     ALDLYLLGEGTDMRLYDKLGAHPMELDDVAGVHFAVLAPNARRVSVVGDFNLWDGRRH
                     AMGVCGQGIWEIFVPGAAAGDRYKFELIGPDGGLLPLKSDPLAFAAELRPQTASIVTD
                     TDSILQPQPAPPDANLRSAPISIYEVHLGSWRRRAEEGHRWLTYRELAAELPAYVRDL
                     GFTHVEFMPVSEFPFDGSWGYQPTGLFAPTSRFGSPADFAALVDACHAAGLAIIIDWV
                     PAHFPDDPHGLRRFDGTPLYEHADPKEGLHPDWNTLVYNFGRREIANFLIASGLFWLD
                     RYGVDGLRADAVASMLYRDYSRREGEWVPNAFGGRENLEAVAFLRRFNTESFSRFPHA
                     TTAAEESTSWPMVSRPVDWGGLGFGYKWNMGWMHDTLEYIAKDPIHRKYHHDRMTFGL
                     LYAFSENFILPLSHDEVVHGKGSLLARMPGDDWQRFANLRAYYGFMFGHPGKKLLFMG
                     CEFAQSQEWRHDHSLDWHLLGDARHAAIQSLIRDLNRLYRSVPALHERDCDASGFEWL
                     VGGDAEHSVFAWLRRGEDERKPCLVVVNFTPEIRHDYPIHVPLPGIWREVLNTDAAIY
                     GGTNAGNGGAVHARGESNELTLVLPPLAALFLVPEG"
     gene            19781..21853
                     /gene="glgX"
                     /locus_tag="VGM72_03955"
     CDS             19781..21853
                     /gene="glgX"
                     /locus_tag="VGM72_03955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEY4264447.1"
                     /translation="MRATQDIARPLGATWDGRGTNFALFSANAEKVELCLFGDGGLKE
                     RARIALPERTGDVWHGYVKDIGPGQLYGYRVHGPYDPERGHRFNANKLLLDPYAQRLS
                     GRLALSGIHFGYRYDDARGDLSFDMRDSASQMVKAVVVAPITSPIHVRRPSIPWADTI
                     LYEAHVKGLTILRQDVPPAWRGTFRALGSPAIVRHLKSLGVTALELLPVQAFIDDWFV
                     TQRGLTNYWGYSTLGFFAPEPHYAAGDPDHILRDTVARLHDAGIEIILDVVYNHTCEG
                     DHLGPTLSFRGIDNVSYYWLRPGEPRFYENFSGTGNALNLSHPAVLQMVVDSLRHWVD
                     IFHIDGFRFDLAATLARGAEGFDAAAPFFAAIRNDPTLTGVKLIAEPWDVGPGGYRAG
                     GFPAEWSEWNDHFRRTQRRYWRGDTNLLGEMARRMAGSDDVFKRASRGPRAGINYVTA
                     HDGFTLADAVSFARKHNAMNGEDNRDGENNNDSTNCGVEGATGDEDILKARQQLRRNL
                     MASLLLAHGVPMLLAGDEAGNSQSGNNNAYCQDNEIGWVKWTGLGCDGEDLMPLIGRL
                     TELRKRFPQLRSGRWLDGRHEDGSHDVLWLTREGAEMTAEDWNTPDGRFLSYLLGVEE
                     NGETPLFIVLNSGPKCVRFTLPPMRKFDEWIAVLNTASRKTDELVYRIGSVLQSPAKS
                     VLVFAGAA"
     gene            complement(21883..23346)
                     /gene="glgA"
                     /locus_tag="VGM72_03960"
     CDS             complement(21883..23346)
                     /gene="glgA"
                     /locus_tag="VGM72_03960"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEY4264448.1"
                     /translation="MSDVKGVSVLSVVSEIFPLIKTGGLGDVTGALPLALREHGVRMV
                     TLVPGYPAVVAALENVHAVLADDDLFGGPARLLAGYAAGHDLLVIDAPHLFDRDGNPY
                     VDADGIDWPDNALRFAALGWVAARIGLGEVAQFRPDIVHCHDWQAGLTLGYLAYDGRP
                     VPGSVVTIHNLAYQGLFPAEMLSALRLPPAAFQVDGIEYYGKIGYLKAGLQFADRITT
                     VSPTYAAEMQTPANGFGLDGLLRGRSDVITGITNGIDVNVWNPLRDTRIPMRFGRTTL
                     SKRTANKAHLQRRFALEENPERLLLGVVSRLAWQKGLDILADALPILLDTGAQLVVLG
                     TGEQALERRLVSLVESHPGRVGCIVGYNEDLAHLMQAGADAMLVPSRYEPCGLTQLCA
                     LQYGAVPVVAKVGGLADTIYDPEETSADAQRATGVQFSPVTQDALEAALIRTSQIWSR
                     KKQWKNMQLNGMRTDVSWKKSAALYARLYVDLLAAKN"
     gene            complement(23343..24608)
                     /gene="glgC"
                     /locus_tag="VGM72_03965"
     CDS             complement(23343..24608)
                     /gene="glgC"
                     /locus_tag="VGM72_03965"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF001947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEY4264449.1"
                     /translation="MASPLSAAPYSRSAMAYVLAGGRGSRLLELTDWRAKPAVFFGCK
                     SRIIDFALSNALNSGIKRIGVATQYKAHSLIRHLQRGWNFFRLERNESFDILPASQRV
                     SETGWYAGTADAIYQNIDIIESYAPKHIAVLAGDHVYKMDYELMFQQHVEQGADVTIG
                     CLEVPLAEASGFGIMHVDANDRILSFIEKPKVPPPMPTKSDHALASMGVYVFETRFLI
                     DQLRRDAADPNSSRDFGKDIIPYLVRNGKAVAHHYSRSCVRTPGQTHAYWRDVGTLDA
                     YFAANMDLTDIVPQLDLYDQDWPIWTFSEITPPAKFVHDLDGRRGQAVSSLVSGGCIV
                     SGASVKKSLLFTRAWLKSYSHVENAIVLPEVVIERAARLRNVIIDRGVHIPENLVVGE
                     DPDADARRFRRTESGVCLITRSMIDKLAS"
     gene            complement(24706..27234)
                     /locus_tag="VGM72_03970"
     CDS             complement(24706..27234)
                     /locus_tag="VGM72_03970"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEY4264450.1"
                     /translation="MGQGEGRSEPSGSHETGFSPSGPPSDAEIEEVKSAVLAKLALTV
                     GKDAASASDRDWFVATALAIRDRVMHRWLASDRESQAKGSKRVYYLSLEFLIGRLLAD
                     VLCNLGLMELFRAALGDLGVDLGVLRAAEPDAALGNGGLGRLAACFMESMATLGIPAC
                     GYGIRYQHGLFRQVIRDGIQQEYPEEWLSFGNPWEFARPDVTYDIGFGGRVETAAWPR
                     GAVRSIWHPAETIQAVAYDIPIVGWRGGHVNPLRLWSARAADPLRLDAFNSGDHFGAF
                     SDLARAEAISKFLYPSDATEAGRELRLRQEYFFVSASLQDLVQRHLKTYGDLLQLPSR
                     AAIQLNDTHPSLAVAELLRILLDVHGVPWRDAWQVAVKTLSYTNHTLLPEALESWPVS
                     VFETMLPRHMQIIYRINAEHLEQAKRNAAFSPDLLSAVSLIDEYGGRRVRMGHLAFVG
                     SHHVNGVSALHTDLMKKTVFRDLHTLYPERIVNKTNGITFRRWLMQANPELLEIVRKT
                     CGDVVLDNPAYLARLADRADDPTLQRLIGEAKHRNKMALSRLIGNRLGLRVDPNAIFD
                     VQIKRVHEYKRQLLNLLETVAMYDAIRANPSGNWVPRVKIFAGKAAPSYTQAKLIIRL
                     INDVGKTINGDASIRNLLSVAYLPDYNVSMAEAIIPAADLSEQISTAGMEASGTGNMK
                     LALNGALTIGTLDGANIEIREQVDDENIFIFGLKANEVEERRRYGLDAGAAITASSKL
                     SDVISAIESGFYSPDEPHRFTALMQALRYSDYYMVASDFEAYFQAQRQVDELWISKAR
                     WMRASIFNIARMHWFSSDRAIGEYAREIWNVPFEAPGPGAGTGT"
     gene            27738..28367
                     /locus_tag="VGM72_03975"
     CDS             27738..28367
                     /locus_tag="VGM72_03975"
                     /inference="COORDINATES: protein motif:HMM:NF016398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HEY4264451.1"
                     /translation="MPTEGGIPVIPLAELEKDALTEIANIAMGRAATSLGQMVNGEVR
                     LTVPSIDIVPNERASMIVAKSDDLRLIAVRQDFRGLFSGRAILIFPEASSLDLIQIIV
                     GRQLSHEDILDLEEEALAETGNIILNSWVATLANLLKHNLTMSLPIVVHGNSTKMFEG
                     TGESAQLVLFLHINFSVSETTISGYLALLMDIPSMDALRALIADYISSF"
     gene            complement(28372..30696)
                     /locus_tag="VGM72_03980"
     CDS             complement(28372..30696)
                     /locus_tag="VGM72_03980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY4264452.1"
                     /translation="MFAVEDQLSADILDALDTGVMLLDGQRRVLVWNRWLVAASGIPV
                     QKALAKTLEELFADSFPHRLMSAIAGVFESGMSSFLTHSLHPSLLPLKTRAGEYLIHS
                     ISVTPLGSRPHVRCLLQIADLTVAVHRERILRERQNARYDAVVESAPDAILTLDSDGI
                     IQLVNPAAANHFQYTMQELIGRPVIVLLKDRTIWSDVWNSVVKGGPSRSIRVLGQRKD
                     GSVTYLEVSASRWLSDSRVFVTVIFHDMNVRRAAEEALHRLNRTLEERVAERTADRDR
                     MWRLSTDVMVVARLDGTINAVNPAWHHLLGWEEGDMVGSNIEAFIVPEDRPQFEIARA
                     ALSASSAPKLFEVTLLTRNRATRRIEWSAVASDNLLQAVGRDVTAEREAEKALRGAEE
                     ALRQAQKMEAIGQLTGGIAHDFNNLLTGIIGAMDALKRRIAVGRYDDLARFMDAAISS
                     SNRAAALTHRLLAFARRQPLNPQAVEANRLVRGMEDLLRRTLGEQVKLVTDLAPDLWP
                     AFTDAHQLENALLNLAINARDAMPTGGQLTIATRNILVAEAEHHGQDEIDPGDYTVIA
                     VSDTGVGMSPEVVPKIFEPFFTTKPIGQGTGLGLSMIYGFAKQSRGHIRIESTLGRGT
                     QASLYLPRYMGPLMPDFLLDTRDAPLGKGEAVLLVEDDSSVRLLIGEVLRDLGYACIE
                     ASDGQAALPMLTSNARLDLMITDVGLPGMNGRELAEIARQHRPSLKILFVTGYAEHAT
                     GKHPFLEQGMEMVTKPFMLDALALKIRQMLADSR"
     gene            complement(30701..31285)
                     /locus_tag="VGM72_03985"
     CDS             complement(30701..31285)
                     /locus_tag="VGM72_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264453.1"
                     /translation="MNIGVSRAAVSLQEMVSQQVHLAVPMIALVSRSEAIRLLREREP
                     DNLIAVQQTFEGSIAGRALLIFPQTKSLELVRAITKSELSLEDIIELEQEALAETGNI
                     ILNGCLATIANLLKCSLRMTLPEILHGGGSDFFAMASLPTSEDVVMFLYIDFTVREHD
                     IRGYICMLMDMPSLKILRGLIRNFLERTTDQAEF"
     gene            complement(31336..31719)
                     /locus_tag="VGM72_03990"
     CDS             complement(31336..31719)
                     /locus_tag="VGM72_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4264454.1"
                     /translation="MTFRILVVDDSKLARMAVTKALNALYPECERMDATNADEALALA
                     RQTPFDVALLDFNMPGKSGLDLAADLRALNPKLALAVVSANHQDEILRRARELGAAFL
                     MKPLTTQALAHFLSDALAHSGTPGR"
     gene            complement(31792..32034)
                     /locus_tag="VGM72_03995"
     CDS             complement(31792..32034)
                     /locus_tag="VGM72_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264455.1"
                     /translation="MTVRLLDSATIVLEGDCPVEDAAVLLERLLAAPNAIVDWRRCSK
                     AHLAVIQVLMTAKVTMHGPPGDGFLAFWVQPLLSRG"
     gene            complement(32031..>33458)
                     /locus_tag="VGM72_04000"
     CDS             complement(32031..>33458)
                     /locus_tag="VGM72_04000"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEY4264456.1"
                     /translation="QIQLLAENLEDGFAGRLASAGRVGANVLRHRDRFEDADQIESAL
                     LQSQIHHEPRFLSEALKAALNADAAGALPPPESHARPREEIAARSLRVDVARIDALVK
                     LTSELTVVKNAIGHAAQLAQAGGDLKTIAAVLKEGHALLEARVAELQHSVLSIRVLPL
                     RHVFQRFPRLVRELSESLGKPAKLIIEGEETEADKTVVEALFEPLLHIIRNAVDHGIE
                     PANDRVSRGKPALASIHLRAARQGQHVLVEVRDDGAGIDVARLRRVAVERGVASAEAA
                     RSMSDQEAVQLIFAAGFSTAAQITGLSGRGVGMDAVRAAAERLGGKVTVETQWGHGTA
                     VRLILPFTVMMTRVMTVEAGGQVFGIPLDAVLESVRIPRDRIVPIGAGRAFVLRNRTV
                     PVVNLAQSLKPDLPGATGRDAKIVITAMGRQVGGIEVDRIGEQSDVMMTPMEGLLAGV
                     RGVSGTTLLGDGRVLIVLDVQDILE"
BASE COUNT         6913 a        10178 c         9723 g         6644 t
ORIGIN      
        1 cgccgccgcc ccgcggctat tgaaacttat ggcgggctgc gcaataaccc cacccttgct
       61 tggagcgcgg gcgtagctca ggggtagagc ataaccttgc caaggttagg gtcgggcgtt
      121 cgaatcgcct cgcccgctcc agattttact cagcgaaatc agcgacttat tgcaccatca
      181 tccgagtggt tctggccgtt tttccgggaa actcgggggg agcttggggc ccgttgcagc
      241 cagcgtccat ttcatgggca gacagtcgtg cttttccagc cgacgaaaat gttcgtgcga
      301 gatataagac ttttatcttg gcaacaaatg attctcgcca atcgcacggc ggcatttcta
      361 agcccaattt gggccagttc ttggattgat cgccggatcg ccgcaatcct ccactgctcg
      421 gcgagcagac gattgcgaag tgctcaaagc ttatggatcg cgagacgtga ttgatgttgg
      481 cgcttacaaa agcgtggcgg ccgcgatggc gaggccatgt cacgacggtg gcctcagacc
      541 gaacccgcac cggcgccagg cacgtctagc gacgaactgc tcgaccacga gcgaaccccg
      601 tcaacccctc cgcgcccaat gcgcaccagc tcccatttgt aggaattacc ctaatcatat
      661 gtagttttat tcctacaaat cgtgtagaaa aagtaagttc cggggccaag caaagcgaac
      721 cctgccattg caattcaaga agggcgtacc ccggcctatc acgcaatctc gtgaagcggc
      781 actcagagga gaaagcccat gaagacggtc atcatccaaa cagccacagc attggttctg
      841 acgacgtttt ttgccgtctc ggcggatgct caaaatcagg gctccgccgg accgccggtt
      901 ctcgcgagca agctcgcccc tgcgagatcc ggcagcacgc agcttctcgt gacgtcaagc
      961 gccttcggtt caggcagcga actcgacgat aaattcacgc agaacgggga caacatgtct
     1021 ccaccgcttg cctggaccaa gggccccccg ggaacgcaat attatgcagt gctggccgaa
     1081 gacgccggcg tggatcgcgc cgaaccgatc gtgcactggg ttatctacaa tattccgagt
     1141 tcgatgaggg ggctgccgca gaatgtgcca acggacgcca cgctcgaaaa cggcgcggtg
     1201 caaggcaaga atgtccgcgg cgccgccggg ttcatcggcc ccaagccgcc caaaggacag
     1261 acccatccct atcactttga ggttttcgca ctgaacgcca agttgaacct gcccgctaac
     1321 gcggaccgca acgcagtggt tggcgcgatg aggaaccatg ttctcgcaca gggcgacctc
     1381 atggccatgt ataccggcaa ataatttcca tctgcatcga cacggcggcg gcgcgcgtca
     1441 cgggcccgcg ccgccgcggg tgccggcccc atgcacgttc gcgaggcgcg gccacgccgt
     1501 cttatcgatc cacgccgcct tatcgaggag gccgaaacga tgtaacttca gggcatgtcc
     1561 accagaatgt tcaaattcct gcagtcgctc tcaccgccag tcctgcttac gacccttacc
     1621 ggattgacgc ttgtgatcgg ctatttcgat tatcgtgcag gaaccgacac cacatttacg
     1681 gcgatttatc tgtttccgat aggtgccgca gcctggttct tcgatcgccg gactgcctct
     1741 gtgctcgctg ctgtgagttc gatactttcg gtaagcgggg acatggaggc cggcgcgcat
     1801 tacgcgagcc tgtggattcc gctatggaat atcgcggcgc gttttgccgt ttttgtgttc
     1861 gccgcgcatc tgatcggtgc gcttcgcaaa ctgaatgccg atttggaaaa gcgggcggcg
     1921 gaacgggcgg caaagctgac ggaagaaatc gccatgcgcg aacggctcca acgcgaactg
     1981 ctgcaaatca gcgaacgcga acaggagcgc gtgggccacg acattcatga cagtctttgt
     2041 cagcacctca ccggcacggc gcttgccgca caggttctcg ccgagagcct tcagtcgcgc
     2101 aatccggcgg aacaggataa tgccgtgcgc gtcgtcgaac tcgtcgagga agggatcgtt
     2161 ctcgcccgca atctcgcgag aggattgaac gccgtcgaag tgtccaacaa cggattgatg
     2221 gccgcgctta ccgattttgc cgcgagcaca agcgatttgt tcaatgtgtc ttgccgtttc
     2281 gaatgttccg aaccgttttt gatggacgat ccgactgcgg cggtccatct ctaccgcatc
     2341 gcgcaggagg cggtgggcaa tgccatcaag catggtgagg cgaagcgcat cgtcatccgg
     2401 ctcgaacgct cgaatgcggg acgcgtgctg cgcgtgatcg acgatggcac cggagtgcgg
     2461 gcctcgcgtc tggacggaaa ggccaagggc atgggtctgc gcatcatgtc ctatcgatcc
     2521 gagcttatcg gaggccgcat ccatatccag aggcgcactc cggccggcac ggaggtaacc
     2581 tgttcgctgc cgttggaaga agccgcgcaa tgacatcggc caaagccaga atcgtcatcg
     2641 tggacgatca ccccctggtc agggagtggc tgaccgcctt gatcgagaag catccggacc
     2701 tcaccgtttg cggcgaggcc gacaacgctg caaccgcgct gagaatcatt tccgaagatc
     2761 cacccgatct tgccatcgtc gatctttccc tcggaagcaa atcgggaatc gagctcatac
     2821 agtcgatccg ttcgtcgcat ccgggcgtcg ccatgatcgt gctgtcgatg tacgacgagc
     2881 aggtttacgc ggagcactgc atcagggcag gcgcgcgcgg ctatatcatg aagcgcgaga
     2941 cggcgaagaa catcatcgat gcaattcacg aagtccttgc gggaaacctc tacctcagca
     3001 agcgcatgac cgaacttatg gttcaaaagg cggtttcagc cgacgcgtca aacggcggcg
     3061 taccgctcgc acagcttagc gagcgggaat tgcaggtttt caagctgctg ggccagagcc
     3121 gtgagatcga tgagatcgcc aagatgctcg atgtcaacat caagaccgtg catacctact
     3181 gcgcgcgcat taaggaaaag atcaaggtca gcaccaccgc cgaactgctg cacgaagcgc
     3241 ggctttggca aggggcgctt ccgccggcct gacggtgcgc gggacgcata tccgagcgtc
     3301 acggggcacg cttctgcgtg aggatatatg cgatgacctc cgaggcttcc acatcggaga
     3361 tagatgtcgt ggtggaaatc gaagtccttt gcggcgcgtc cttgttgcga tgcgtccggt
     3421 ccaacgcttc acgcaaggac acgaccgaca cgccaggccg attcgcgatg tcctcgaagc
     3481 tgggaatcgc atccccggtg accggccgga attcctgttg cgggctcgcg acatggcatc
     3541 cggtacatga ttccaacgcg atttcgtgac cggcccgaat cgcgctttcc tgggagggat
     3601 tctggccggc tgttccggcc tcttgtgcca tggctggcgc gatgaaaacc gccgcgccca
     3661 gcgccgcgta aagcgcaaaa tcacgcattg tcggtgtccc tgtaaagtcc tttaccagtc
     3721 tgacgtcttc caattgttag aaatcttaca tgatttgtag gcttgaacct acgcacgcgt
     3781 aggctaatag tttccgcaca gagacccgtg tcttccagag ccttctcttc gagagggggc
     3841 ggaagccgca ggacatttgc ccttcaagaa ggaaatccca tgcgtcgtga cgcatgcgaa
     3901 acaggcgcag cgtgcggacg gacgaacgga cgatatggcc gcgcgcgcac gttgctcaac
     3961 cccacttacc cacgcgtaat gcgctctggg gacctgacct gaatgtatgc gatcgtccgc
     4021 cttgcgctca gataccgcta caccgcggtc gtgatagcga tattcatatc catcttcggc
     4081 atctcggcag ccgtgacaac gccggcggac atttttcccg atatcaaaat ccccgttatt
     4141 gctgtcgtct ggacctatcg gggactttcc ccgaacgata tggccgggcg cgtgatctat
     4201 tactacgagc gccagctctc cacctatgtg aacgatatcg aacatatgga gagccagtct
     4261 ctgttcggca tcgggatcgt aaagatttat ttccatccgg gtgccgatat tcgcacggcg
     4321 accgccgaag tcgattcggt ctcccagacg gttctcaaac aactgccgcc cggaatcacg
     4381 ccgcccctca ttttgaacta caacgcctcc acggtcccaa tactccagct tgcgctttca
     4441 agtgccacgc tgccggaaca ggagatttac gatctcgggc agaattcgat ccgtccccgg
     4501 ctcgcaacgg ttcagggcac tgcggttccc tcgccctacg gtggaaagga acggcagatc
     4561 cagatcgatc tcgaccctca ggcgcttcag gccaagggct tatccgcgca ggacgttgaa
     4621 accgcgattg ccgcgcagaa ccagatcatc ccggcaggca cgaccaaaat cggccagttc
     4681 gaatacaacg tcaaactcaa tgacagcccc gtcacggtcg cggagctgaa cgatctgccg
     4741 atccgatccc aggacggctc gacgacgtac atccgcgaca tcggccatgt aagagacggc
     4801 tttccgccac agcgcaatat cgtgcgggtc gatggccggc gcgcagcctt gatgaccatt
     4861 ttgaaaagcg gaagcgtgtc cacgctcgcg atcgtggacg gcgtcaaatc ccttttgccg
     4921 cggctggagg agacgctgcc gaaatcattg cgtatcgcgg cgattggcga ccaatcgctt
     4981 ttcgtcaagg ccgcggtatc ggacgtgatc cgcgagggcg tcattgcggc cgctctcacc
     5041 agtttgatga ttctggtctt cctcggaagc tggcgctcga cgcttatcat cgcgacatcg
     5101 atcccgctcg cgatcctcgc atcgatcgcg gcgctatccg cattgggcca gacgctgaat
     5161 atcatgacgc tgggaggact tgcgctcgcg gtcggcattc tggtcgacga cgccaccgtg
     5221 acgatcgaga gcatcaactg gcatctggag cggggcaaac ccatccaggc ggcaatattc
     5281 gacggggcgc gccagatcgt cgcccccgcc ttcgtgtcct tgctttgtat ctgcatcgcc
     5341 tttgtgccga tgttcttctt ccccggcgtc tccggctttc tgtttgctcc gatggccgaa
     5401 gccgtcgtgt tcgccatgat cgcttcgttc gtgctctccc gcacgctcgt tccggtcatg
     5461 gcgaaccatc tgctcgtggc ccacggcgga gcccacgccg tcgcggtcat gcgcagccat
     5521 gattccgaaa ccgggatcag gaccacccgc aatcccttca ttctctttca gcatggtttc
     5581 gcgcggcgat tcgatcgcgc ccgcgatgga tatcgcaacc tgctcgagct ggcgctcggc
     5641 aaccgccgca ttttcatcgc cggtttcatg cttgcggttc tcgcgtcttt tgcgctgata
     5701 ccgtttctcg ggcaggactt cttcccgtcc gtcgattccg gccaaatcag tctgcacgtg
     5761 cgcgcgcctg tgggaacccg gatcgaggag accgcggccc tgttcgaccg tatcgaaaac
     5821 aacatccgga gcgtaatccc gccgaagcag cttggtacga tcgtggataa tatcggcttg
     5881 ccggttagcg gtctcaaccg tgcctatttg aacactggcg gaattggccc cgaagacggc
     5941 gatatccttg tcagtttgac gcccggtcac gccgcgacag caggttatgt caagacactt
     6001 cgcggcctgc tgccgcggag ctttcccggc agcgggtttg catttcttcc cgccgacatc
     6061 gtcagtcaga ttctgaactt cggcgcaccg gccccaatcg atgtgacggt ctcaggcccc
     6121 aacaaaacgg cgaacgaaac ctatgcgcag gaattgctgc gggggatcgc caagattccc
     6181 ggcacggcgg acgctcgcat cgagcaatct tcgcattacc cggagctcgc catcgacatc
     6241 gaccgcaccc gggccaatca gatgggggtc accgagcgcg acgtgacgaa cagcctcgtc
     6301 gtcaatctgg ccagcagttt ccaggtcgcc cccgcgttct ggctcgatcc tagcaatggg
     6361 gcgtcctatc cgattgtcgc acaaacaccg caatatcggc tggattcgct ttccaagatg
     6421 gaaaatctcc ccgtgaccgg cagcaagcct ggaaacatgc aggtgctcgg ggcactgggc
     6481 acgatccacc gcgaagacag cgatgcgatc gtttcccatt atgcgattca accgaccatc
     6541 gacatctacg cggcgacgca aggccgcgat cttggcgcgg tcgccgccga catccaacaa
     6601 ttgatccagc agaccggcaa agatcgccca aagggcgcga cagtcacgat gcgcggtcag
     6661 gtcgaaacca tgaacagcgc tttttccggt ctgttcatcg gtttggccga agcggtcatt
     6721 ctcatctatc tgctcatcgt cgtgaatttc caatcctggg ccgatccatt tgtcatcgtg
     6781 agcgctttgc catccgcttt ggcgggaatc gtgtggatgc tgttcgcaac cgacaccacg
     6841 ctgagcgttc cggccctgac gggcgccatc atgtgcatgg gcgtcgccac ggcgaacagc
     6901 gttctggtgg tgagcttcgc gcgggaacaa ttggagctga cgggcgatgc ggtcggcgcc
     6961 gcgctggaag cgggctacac gcggttccgt ccggtgttga tgaccgcgct cgccatgatc
     7021 atcgggatgg ccccaatggc gctcggactg ggagagggcg gagagcagaa cgcaccgctc
     7081 ggccgcgccg tgatcggcgg gcttatcgtt gccaccgccg ccacactgct tttcgttccc
     7141 gttgcattca gcctcgtcca cggtcgcacg aaatcccaac cgcttccgtc ggatgcagcg
     7201 tccctgccgg aaagagacgt ctatgcccca gcagaatgat atggaaaaac cgcggcgccg
     7261 gacgataagg cggctcaagc agaccgctgc ggtgggggtc ggcgtcgctg ccatcatcgt
     7321 cgcgagcggc atatgggtgc gcctccacgc gagcagcgag caggcaagct ggaccacgga
     7381 gcagacggtt cccacggtca atgtcgtgca tccctccggt ctcggcggca atggcgtgct
     7441 cacgctgccg ggaaatctgc aagccttcta caccgcgcgt atctacgcac gcgttccggg
     7501 atatgtgcgg caatggtaca aggacatcgg cgcggtggtg catcgcggcg atatcctcgc
     7561 cgacatcgat acgcccgaac tggaccagca aatcgagcag gtgaaagccg acctcgccaa
     7621 tgctcaggcg gcgcagaagc tggcggaaac gacggccgcc cgatgggcga acctgctggc
     7681 ggtggatgcc gtttccaaac aggaagcgga ggagaaagcg ggcgacctcg cggttaagaa
     7741 cgctcaggtc aatgccgcca aggcaaatgt cgaccgcttg gagagcatga agacattcgc
     7801 ccgcatcacc gccccttttg acggcgtggt gaccgaacgt gcaaccgatg tcggagcgct
     7861 ggtcaatgca ggcgccggat cgaccggctc gcccctcttc accgtttcgg acctgcacaa
     7921 gatccgcgtc tacgtccagg tgccacagaa ctattcggcg cagattcaca atcatatgaa
     7981 ggccgagttg agtctgccgg aatatcccgg cgagaccttc tccgcccagt tggtttcgac
     8041 ctcggatgcg atcagcgacc aatcgaacac tctcttggtt cagcttgagg ccgacaatcg
     8101 gatgggaaag ctcaaacccg gagaatatgc gcaggtcagc ctgctgcttc ccgcggataa
     8161 gggcatgctt cgcattccat cgagcgcgtt ggtgttccgg ggcgaggggc ttcaggtggc
     8221 gacggtggga caggacaatc acgtcgtgat gaagccgatc cgtattgcca atgatctggg
     8281 tccccaggtc gaggtgagtt ccgggttgga tgcctcggat aaagtcatcg acaatccgcc
     8341 cgattccctg gccatgggag acgccgtgcg cgtggccggc ccgcaggatc aacagcacgc
     8401 ggatgccaaa taggatgcgc gccgctgggc tcgtttcggc gcttcttggg gcgtcatggc
     8461 ttgcaggatg ctcgcttgca ccgcgctacg aggttcccgc gtccaccatc cccgtgtcct
     8521 tcaaggaaaa cggaatgtgg acgcaggcaa cgccgcaaga tgcgatcccg cgcggcaatt
     8581 ggtggtcggt ctatcgcgat cccacgctcg cggcattgga agcgcggatt gagggatcga
     8641 acccaacgct tgcccaagcg ctggcgcgct acgatgaagc gagcgcctat ctggcggagg
     8701 cacaggcgag tctgtttccc ctggtcggat cgaacgcgca aatcagccaa aaccggcagt
     8761 cggacgaacg gccgctgcgc ggctccaatc agcccaatga ctacgccaac aattccctta
     8821 cggcgtcgat ctcctacgat ctcgatttct ggggccggat tcgcaacatc gtgcaggccg
     8881 ggaaggccga acaacaggca caggccgccg atttggaatc ggtgcggctc agcctcgagg
     8941 cacaactcgc cgacgattac gtgaaattgc gcgccatgga cgcggagctc gatctgtacc
     9001 gggtcacgct tgccgcctat gaacgggcct tcaatctgat cgatcagcgt tatacccagg
     9061 gggcggcttc gggtctggat ctgggccgtg cccagactca actcgaaagc gcgcgcgcgg
     9121 aaatctccaa tgtcgccgcg caacgcgcgc tttacgaaca cgctattgcg agcctcgtgg
     9181 gcacaccggc gtctgcattt tcgcttacgc ctgaaatcgt aacgctgaat gttccgaacg
     9241 tccccacggg gttgccgtcc actcttttgc aacgccgccc cgacatcgca gcggcggaaa
     9301 gacgggttgc ggaggccaat gcccagatcg gcgttgcgcg cgccgcgttt ttcccgtcga
     9361 ttatcctggc ggcgacagcg ggatttcaag attcgggcgg cgtgggcgtg ggactggttt
     9421 cggcgcccaa tctcctatgg tcggtcggtc ctgccgcggc gctgaccctt ttcgatgccg
     9481 gagccagaca agcgcgtgtg gatgcgacgg ttgcccggcg caaccagacc gccgatatct
     9541 atcgcaccgc tgttctgcag gcctttcagg acgtcgagga caatctggcg gttttgaaca
     9601 atctcgcagc ggccgcaacc gcggaagacg ccgccgttgg cgcggcggca cgaaccgaga
     9661 cgctttccat ggatcgctat gatctgggtg cggcaagcta tctcgatgtg gtgacggctc
     9721 agacggcggc cttgcaggcc agattgtcgg cactcgattt tcatacgcga cgtcttgaat
     9781 ccagcgtaag actcatccgt gcgctgggcg gaggctggcc cgcgtccgaa gcttatgacg
     9841 tcaccgcgct gccctgaacc gacgctacac tgaaccggtt ggccgaacct ctcgcatgag
     9901 aggcccggcc gctggatttg ttattggaac ggataatagc ggaagctggt gaacaccatg
     9961 ttgtcggagg tatgcggaga gcccccaacc ccgttgaagg ccttcagata ggtgtaggaa
    10021 tactgcacgc cgattctcac ccggccgaaa tcgccgtcat aggtgttatc ccacaagccg
    10081 acattgatct gctcgaccgt ctggatgttg cctgtacagc tcgcggtgga agagaaactg
    10141 gagcagccgg tattgttgaa agccggattg ccgtaaccga gattctgcga agtgccgttg
    10201 agcgtgccac tgaaaaaatc cgaatcatcg tgttcgcggc ccgcgaagac atagatgtcg
    10261 agcgcgcggg tggcatgcca tgtcaggccg atcatctcga tgtcctgtct cagcggcgcg
    10321 aaggtgccgt tggcattgta ggtcacgtct ggaagctgac tgctgccgta acggccgatg
    10381 ccctcgccaa acagagtgtc gaattgaacg tccagcgtac cgggcacaag tttgatcagg
    10441 cccgcgacgc cgccgccgcc gcccgagaca ttatggttcg atacgtcggt cagggacgtt
    10501 ccgaagcgat cgtaaaagtc gcggtacatg ccgacggctt caagatgcac gttgcggccg
    10561 aaaaacgacg gctcccatgc gaccttgccg atcagatcgg gaacatggtt gagcgagagc
    10621 gtgatgcccg gattgaattc ggacagaccc acattcgtac tgccgccatt gaagccgctg
    10681 ccactgctga ccgtaagtcc ggtataccca gcgggtgcgg ttccaccgat ggtcgtttgc
    10741 gggttctcga tcgacagacc aacccagaac tcgttgccga aattcttgac gacgcgaagc
    10801 tgggcctgac gcgtccagac gaaaccggcg gaatattggg cttcgatggt gggcggagca
    10861 agttcggtgc gttccgacat cccctggccg ttcaacgtcg caagcgacca gctttggccg
    10921 aataggacgt ggatgccgag gtcgctccaa tccgccgatc cgtatagatt gcggatgcgt
    10981 ggcgcgtagg aattgctttc attcgagttc gcgctggcgg gaccacccat gaaatcgaac
    11041 tcgccataca tggcaaagtg cgtattggga tcgaaatcgc cttcagcgag accttgaacc
    11101 cggctttgcc gggcggtcat gcgaaattcg ccggtgtgcc caacgccgac attgttgccg
    11161 gtgccaccgg ggccaggacc cgcaaaggga attttggcga agcccggaga gccgatatcg
    11221 gactccagat tgtttgagcg ccagacggtt tcggccgcca gaaagccgcc cagcgagagc
    11281 gtgatgccct tgaagtgaat tttcttgtct accggcgcgg caggcgccgt cgcggcttgt
    11341 ccgaatgccg gaccggcgag cgccacgcat gtgattgtcc ccgccaatat gcttttgaag
    11401 ttcattttat ccccgcgatt gtcgattcca accgctagcc cggacagcac ctgtccccgc
    11461 caacggtgcg acgttgcgat aaatgctgtt tgtgacaccg gattgaatga tgcatgaacg
    11521 attcatgacg gcggctctcg ggaattgccc gcgcggtccg ggggcactgt cacaaaagct
    11581 tccgatatgg gtgatatcag cgtcgtctga atttgagctc atctgcccga aagatttcgg
    11641 ctagccattt cggcggacga acttcgcgcg aagcaatcac gggagaatcc caatgaggct
    11701 atattttagt ttccccgcca gcatccttgc cggcgttatc gcagtgggcc tggcaaccga
    11761 tgtctctgcc gcagacatct ccggtgccgg atccacattc gcgtttccca tctacggcaa
    11821 atgggcggcc gcatatcaga tggtcagtgg atcacggttg aattatcaat ccatcggctc
    11881 gggcggcggc atcaaacaga tcgaatccaa gacggtcacg ttcggcgcca ccgatatgcc
    11941 gctcccgacc aaggaattgg accagtatgg tcttattcaa tttccaaccg tcatcggcgg
    12001 cgtcgtgccc gtggtgaata ttgccggcgt gaagtccggc gatgtggttc tggacggccc
    12061 gactttggcg gatatttatc ttggcaagat caaaacgtgg aacgccgcgg ccatcaaaaa
    12121 actgaatccg accgtgaatt tgccgaatca ggccatcgtc gtcatccatc gctccgatgc
    12181 ctctggaacc gtctatatct tcaccaacta tctttccaag gtcagcgccg aatggaaaga
    12241 ccacgtcggc aatgccactg gagtcgattg gccggccggc attggcgcca agggaagcga
    12301 gggtgtcgcc ggcaacgtga tgcagtcgaa cggggctatc gggttcgtgg aatatgccta
    12361 tgccaagcag aacaatcttt cttacacacg catgctgaac cacgatggca aagcggtaag
    12421 cccggtggcg gcggctttcg cggcggctgc cagtggcgcg gcgtggaacc cggcggacgg
    12481 tttcgcaacc atgctgaccg accaggccgg gcaaggcagt tggccgatct caggggcgac
    12541 atgggttctc ttctacaaga acccgccgga caaagccgca accgcagagg gtctcaaatt
    12601 tttccaatgg tctttcaaga acggcgacca gatcgccgcc ggcctggact acgtgccttt
    12661 cccgacggct gtgaaggaca agatcgaaag cagctggact caggtgcagg gctggtccgc
    12721 ccgttagacc gcggactttt ccggactcgg ctttcacaaa gagaaaatag cggagcggcg
    12781 ctccgctatt tttttgtgac gcctcgcgtc aacagccgtt ggcggagcgg gtaatctgcg
    12841 cggtccgccg cggacgcaga tagaaggaat catgcaacgg ccgggtgccg tagatcagat
    12901 aaccggcggg cgacgaataa ttgtaagtca cttcggtgaa gatcacgctg ccgccgcccc
    12961 cgctggtcac caggcccgca ggaatagcga caaccgagtt caccaagcgc ggcgtggtat
    13021 tgagcgcatc gctccaggcc actttcaccg tgccggttcc cgtatcgatc aggcttgata
    13081 tcgtgatctg catgttgttc tggggatatg gaaacatgat cgcgatcatt gccgaaaaga
    13141 tgtcgcttat ctcggcattg cagaccgcag attcccgggc cgtcagatcg gccgcagaac
    13201 ttgccacgga cgtgaccttg ctgctggcgg taaaagcatc cgcgatttcc gccacgccga
    13261 aatacatcac gatcatgatg ggagcgatca gggcgaattc cgtggcgatg acgccttcgc
    13321 gcgcgctgtg aaagcggcga aagaatgtga ccaaggatcg cggacacaag ccgcgcgcca
    13381 tttcaatagg gctcattttt gaatgccgcg ctgaaggcga tgagacgcac accactgccc
    13441 tttcccatgt tctcgaaata ttgggcaaat agcggcgtgt agagctgcca tttgtacaat
    13501 acgcgcacca agacgatcga gcttccagtc gcgtcgctcg accctcccgg ctggtaggca
    13561 ttgagattgg ggttgaggtt tcccgccgca tccaaggccg gaggaaacgc cgagccgctg
    13621 aaattggcga aggaccgcac gtcgatcagc aaattcgtgg ggtcgcagga aagcaaaatc
    13681 tttacctgat cgcacacggc gttacgaaaa tcggcttgcg tcatgctgcc cgtctgcgcc
    13741 tgtccggtac ggatcaagcg cccggtgtct attacggcgt tgtcgaggat catgttcgcg
    13801 aagaacacca tgctggtttc cagaatggcg aaggtcagca acatgaatac gggagcgatc
    13861 attgcgaact ccgccgccgc ggcgcccgaa ttttcccgcc ttctgaatat cgaacaaaca
    13921 ctgcgcatga acgatggtcc ttgacccaga tcgcggcgcc gtcttctgga acgtttttcc
    13981 ttacgcgcca atccgccaca gataaaggcg atgaacgaac tagcgtgaat gaactcttaa
    14041 ccggcttggg cgaggtttcg cctcaaatga aatttttaac attctgtgtg cctgcttaac
    14101 cggacactaa gaacgaattg cttcggcacg ggagtttccg gcgtgcttgc gtatcatgcg
    14161 atcatgcatt cggtctggag gttggtcatg cgcaagaatc gtaactttat cccgcaagct
    14221 tctcattggt ggaaaaagtt cgttgccgcg aagagcggga gcaccgcaat gatttttgcg
    14281 ctcagttcgc tgacattgtt ttcagcggca ggcgcaggca tcgatatgtc gcgcgccatg
    14341 aatgcgaaat cacggctggc cggcgcgctc gatgcggctg cgcttgctgt cggcaccacg
    14401 aacgggctta ccacgcctca gctccaaacc ctcgcccaga aatatttcga cgccaactac
    14461 ccggcgacgg cactcggcac gcatacgcca gtcaccgtgg ctgtcaacgg acaaagcatc
    14521 actctcggcg tgaccggcag ggtgccgacc accctgctgc aagttgcggg aatcggcgac
    14581 gtcgacctgt cggtcaccaa tcaggtgaca aaagcggtga cgaaactgcg cgtcgcgctc
    14641 gtgctcgaca ataccggctc gatgtcccaa accgacaata ccggaaccag caagatatcg
    14701 gcgttgaaaa cggcgacgca tcaattgctg acgcagttgc agggcgcagc ggtcaaccct
    14761 ggcgatgtgc aagtctccat cgttccgttt tcgctcgacg tgaattacgg cacgtcgaac
    14821 ccggcggcca cttggatcga ctggaccgat ttcgatgcgc aatcacgcca accgtcctac
    14881 agtgtcggcc ccggatcgac ctgtccgtac accaacggaa ccgacggatt caaatgtgcc
    14941 aaaagtcctg tcaatgatcc aaactgtaac gtcggctcca acaacacgtg tgtcagcacg
    15001 gtcccttcat cgggaaccta taagggttac atctgcccaa gcgcccatca aaccgatgcc
    15061 agcaatggcc gcggcggaca tttttacaat ggctgctggg acagtgtgtt ggtttccgga
    15121 agatacacac acacatggca tgggaacact cacaccacgt ggacaggttg cttcatggac
    15181 cgcgcccaga actacgatac gaacaactcg acacccgtag tcgcaactcc tgcgacattg
    15241 ttcccggcgg aaaacaacct ctattgcccg ccggtggcac tgaatgcgct gagctatgat
    15301 tgggcatcct taggcagcaa ggtcgatgcg atgacgccga acggatcgac caaccagaca
    15361 atcgggctcg cctggggctt tcaggcgcta acgtcaacaa accctctgaa tgcgccagcc
    15421 caggacccca ccatccagaa cgtcattgtt ctcttgagcg acggtttgaa cacgcaaaac
    15481 cgctttgccg gtgatggatc gaaccagagt tctgccgtcg atgcccgcat ggctcttgcc
    15541 tgcaccaacg caaaagcggc cggcgtgcga atttatacgg tgctggtaat gtccggaaat
    15601 tcttcgatcc tgcagaattg cgccacggac tcgaagaagt atttttcctt gaatacggcg
    15661 ggccagatcg tcacggcgtt caacaccatc gggaccgagc tcgccaacct gcatctgtcg
    15721 cgctgaacgc agctctaacg gtttcgaaaa caccccttcc ttcatgggaa ggggtgttct
    15781 ctttttaagc gcatctaacg tccacgttcc gctgacagaa tcttggccaa agcgataagg
    15841 cgccggtcgc ctttcagatc ttccagacag acgggaagcc gccggcgcca attgggatgt
    15901 tccgcgatcg tgccggggac attcacctga tccatcatgc ccagcacgtc ctccgccgac
    15961 accatcaaca atcgcgaggg agtctccgac aggaaacgat cgaggcttgg ataatcgagg
    16021 gtatgaaaac cgcgtgacgc cattgttcgg gcgattgcat cacgcgccgc ggcgcgctcc
    16081 tgatcgcttt cgcccgggtc catgccaagc gcccgtttca cggccaagtc ccggctgctc
    16141 atccatccgg cgaaggtggg caggtcgtgc gtcgcaaagg tcacgagtgc gttgcgcgcg
    16201 taatgatccg gcgcgacgaa tcctccatcg gccgcgcgct gaaacagcat cacctggtaa
    16261 gaccagaggc cccaatccgc gagggtctcg cgaaagccgt cgggcaccgt tccgagatcc
    16321 tcgccgatga ccacacatcg atgagcgaga ctttcctgac tcacagccgc caacagaggt
    16381 tcgaacggca atcgcacata cgcgccctga tcggcgcgca tgtcctttgg gatcagatac
    16441 agccgtttga gtcccagaac atgatcaaga cgaatggagc cggcatattg catggacgcg
    16501 gcgagcatgc ggcgaaacgc ggcgcagccc tgctccgcga ggccaatcgg attgatgccc
    16561 gcaagccccc atctttggcc ctccagattg aggcggtcgg ggggcgcgcc gatctccagg
    16621 gccggctcga acacgctttg ctggttccag gcgtcgaagc cgtcgctgcg cacacccacg
    16681 gcgatgtcga gataaagacc tatcggcaac cccaatgccg ccgcctgcgt gctgcacgcc
    16741 cgccattgcg aatcggcgac ccattggaga aattcatgga tgccagtcgc ttcatcctgc
    16801 gaggcccgaa gggccgcgag ccgttcatcc tctggcgttc gccattccgc gggccatgtc
    16861 caccagggac cgtcatgctg ccggcgcagg aattcgaaac aggaaaaccg cagcaaagca
    16921 tggccaccgc gttcccggaa gctcgcgaat tcgcgttttc gccgcgcagt tccatggcgc
    16981 cggaaattgg cataggcaag agaaaaggcg cgcgttttcg ttttgatgag acccacgtaa
    17041 tcgaccaatt cggactggcg aagtgcgctg acatcgtccg ccaggcccgc ctcatcgagg
    17101 ccgggaaatt cctcaatgcg ctccgcatcg atataatggg tattgagaaa aagcctgctg
    17161 ctgggcgaat agggactgta ggaatcggca agattgtcga aaagcgcgtg cagcggatta
    17221 agacctatcg cggatgcgcc gagaccggcg gcgatttcga tgagcatttc gagatcggtg
    17281 aaatccccat ggccccaatt gtgccgtgaa cgcacaccgt agagctgcac cgcaaggccc
    17341 cacatacgct caggcgcatt gccatgcccc tgataggcgg ggtgggatac cgaaacggcc
    17401 ggaaaatcca gagcggtagg tttgccgttc aagccgagaa tttcgatcag ccgggacaac
    17461 acatcttcgg tgacgatgcg atgctgtcca agaccatccc aatattgcgt ttcaatgccc
    17521 caacacgccg cacgctcttc gatgctatcc attgcccacc gatccgctca tagagagcga
    17581 gcttattgag cggggccgca atagtcgata acgctagtgc cgatgcaaac agaacctctt
    17641 ccgcccgccg cctatgccat cgtcgaggga cggcatcacg acccgtttca ctatcttggc
    17701 ccccatgtcg aagacggtgt ttgcttcgtc cgcgtctttc ttccgggtgc ctcacaggtt
    17761 gccgtgatcc tgaaaaacgg acgcgaattt tccctcgcgc gcctgcacga tgcgggcctt
    17821 tttggggcgc ccatttccgg tggagattcc cattaccgtc tacgtgccgt tttcgacgga
    17881 aaaaccgtcg agatggaaga cgcctatcgc ttcaagcgcg tgctgtccgc gttggacctc
    17941 tatctgctgg gcgaaggcac ggatatgcgg ctttacgata agctcggcgc ccatcccatg
    18001 gaactcgatg acgtcgcggg tgtgcatttc gcggtcttgg ctccgaatgc tcggcgcgtc
    18061 agcgtggttg gcgatttcaa tttgtgggat gggcggcggc atgcgatggg ggtctgtggt
    18121 caaggcattt gggaaatctt cgtgccgggg gccgcggcgg gcgacaggta caaattcgaa
    18181 ctcatcggcc cggacggcgg ccttcttcct ctcaaatccg atccgctcgc ctttgcagca
    18241 gagttgcggc cgcagacggc gtccatcgtc accgatacgg attcgattct ccagccgcag
    18301 cccgccccgc ctgacgcgaa cctgcgctcg gcgcccattt cgatttatga ggtccatctc
    18361 ggctcgtggc gccgccgcgc ggaggagggc catcgctggc tcacctatcg cgaactcgcc
    18421 gccgaattgc ccgcctatgt ccgcgatctc ggtttcacgc atgtcgaatt catgccggtg
    18481 agcgaattcc cgttcgacgg ctcgtggggt tatcaaccca ccggattgtt cgcgccgacg
    18541 agccgtttcg gatcgccggc cgacttcgcc gccctggtcg atgcctgtca tgccgcgggt
    18601 ctcgcaatca tcatcgattg ggtgccggcg cattttccgg acgatcccca cgggcttcgc
    18661 cggtttgacg gaacgccgct ttatgagcac gccgatccca aggagggcct tcacccggat
    18721 tggaacacgc ttgtctataa tttcgggcgg cgcgagatcg cgaatttctt gatcgcaagc
    18781 ggtctgttct ggctggaccg ctacggagta gacggcctgc gcgccgacgc ggtggcctcc
    18841 atgctctacc gcgactacag ccggcgcgaa ggcgaatggg tccccaatgc gtttggcggc
    18901 cgcgagaatc tcgaggccgt cgccttccta cgccgtttca acacggagag cttttcgcgt
    18961 tttccgcatg ccacgaccgc cgccgaagaa tccacctcct ggccgatggt ttcacgcccc
    19021 gtcgattggg gcggcctcgg cttcggctat aaatggaaca tgggatggat gcacgacacg
    19081 ctggaataca tcgccaaaga tccgatccac cgcaaatatc atcacgaccg tatgacgttt
    19141 ggattgctct acgcgttcag cgaaaacttc atcctgccgc tgtcccatga cgaggtcgtc
    19201 catggcaagg gctcgctgct ggctcgcatg ccgggcgacg attggcagcg cttcgccaat
    19261 ttgcgggcct attacggctt catgttcggc catcccggca agaagctgct gttcatggga
    19321 tgcgaattcg cgcaaagcca ggaatggcgc cacgatcatt cgctcgattg gcatcttctc
    19381 ggcgatgcgc gccacgccgc gatccaatcg ctgatacgcg atctcaaccg gctttaccgt
    19441 tccgttccgg cgctgcacga gcgcgattgc gatgcttccg gctttgaatg gctcgtcggc
    19501 ggcgacgccg aacacagcgt gttcgcttgg ctgcgaagag gcgaggacga acgcaaaccc
    19561 tgcctcgtcg tcgtcaattt caccccggaa atccggcatg attaccccat ccatgtacca
    19621 ttgccgggga tttggcgcga agtgctcaac accgatgccg cgatctatgg cggaaccaat
    19681 gccggcaatg gcggcgcggt ccacgcgcga ggcgaaagca acgaactgac gctcgtgctg
    19741 ccgcccttgg ccgctttgtt cctcgttccg gaaggctgag atgcgcgcca cccaggacat
    19801 agcgcgtccg ctgggcgcca cctgggacgg acggggtacg aatttcgcgc tgttctccgc
    19861 caatgccgaa aaagtggagc tctgtctttt cggcgatggc ggtctcaagg aacgcgcgcg
    19921 catcgcgctg cctgagcgca ccggagatgt ctggcatgga tatgtgaagg acatcggtcc
    19981 cggtcagctt tatggctatc gcgtgcatgg tccttacgac cccgagcgcg gccatcgctt
    20041 taacgcgaac aaattgctgc tcgatcccta cgcacagcgg ctttccggac gtctcgcgct
    20101 cagcggtatc catttcggct atcgctatga cgatgcgcgt ggcgacctct ccttcgacat
    20161 gcgtgacagt gcaagccaaa tggtgaaagc tgtcgtcgtc gcacccatca cgtccccgat
    20221 acatgttcgc cggccttcaa tcccgtgggc ggacacgatc ctctatgaag cccacgtcaa
    20281 aggattgacg atccttcgcc aggatgttcc accggcctgg cgcggcacgt ttcgcgccct
    20341 tggctcgccg gccattgtcc ggcatctcaa atctcttggc gtcacagcgc tcgaattgct
    20401 tccggtccag gcattcatcg acgattggtt tgtcacccaa cgcggactga cgaattactg
    20461 gggttacagt acgctcgggt tcttcgcgcc cgaaccgcat tatgccgcgg gtgaccccga
    20521 ccatattctt cgcgacacgg tcgcgcggct gcacgatgcc ggcatcgaga tcattctcga
    20581 tgtcgtctac aatcatacct gcgaaggtga ccatctggga cccacactca gtttccgcgg
    20641 catcgacaac gtctcctatt attggctgcg ccccggtgaa ccgcgtttct acgaaaattt
    20701 cagcggcacg ggaaacgcgc tcaatctctc gcatccggcc gtcctgcaaa tggtggtcga
    20761 ttcgctgcgc cattgggtcg acattttcca tatagatggg tttcgtttcg atctcgccgc
    20821 gacgctggcg cgcggagcgg agggtttcga cgccgccgcg ccattctttg ccgcgattcg
    20881 gaatgatcct accctcaccg gcgtcaaact gatcgccgaa ccatgggacg tgggccccgg
    20941 cggctatcgt gccggcggat ttccggcgga atggtccgaa tggaacgatc atttccgccg
    21001 gacccagcgc cgctactggc gcggcgatac aaatcttctg ggcgaaatgg cgcggcggat
    21061 ggcgggttcg gacgatgtgt tcaagcgcgc atcgcgcggc ccgcgcgccg ggatcaatta
    21121 tgtgacggcc catgacggat ttacccttgc cgatgccgtc agcttcgcac gcaaacacaa
    21181 tgcgatgaat ggcgaggaca atcgcgatgg cgaaaacaac aacgacagca ccaattgtgg
    21241 cgttgagggg gccacgggag atgaggatat cctcaaagcg cgccagcaat tgcgccgcaa
    21301 tctgatggcc tcgcttctgc tggcccatgg cgtgcccatg ctgcttgccg gcgacgaagc
    21361 gggcaacagt caatcgggca acaacaatgc ctattgccag gacaatgaga tcggctgggt
    21421 caaatggacc gggctcggct gtgacggcga ggatctcatg ccgctgatcg ggcggctgac
    21481 cgaactgcgc aagcgtttcc cgcaactccg ttccggccgc tggctcgacg gccgccatga
    21541 ggatggctcg cacgatgtcc tttggctaac ccgcgagggc gcggagatga cggccgaaga
    21601 ttggaatact cccgacgggc ggtttctgtc ctatcttctt ggcgtggagg agaacggtga
    21661 gactccgctg ttcatcgtgc tcaattcggg gccgaaatgc gtgcgcttta cgctgcctcc
    21721 catgcgcaaa ttcgacgaat ggatcgccgt tctcaatacc gcatcgcgca aaacggatga
    21781 attggtgtac cggataggat cggtcctgca gtcgccggcg aaatcggtgt tggtttttgc
    21841 cggcgcggcg tgatcgcgga acgacgccgg ctggttcttc ttctaatttt tcgccgccag
    21901 cagatcgaca tagagacgcg cataaagcgc tgcggatttc ttccacgaga catccgtgcg
    21961 catgccgttg agctgcatgt tcttccattg cttcttgcgc gaccagattt gcgatgtgcg
    22021 aatcaatgcc gcttcgagcg cgtcttgcgt caccggactg aactggacac ccgtggcgcg
    22081 ttgtgcgtcc gccgaggttt cttcagggtc gtagatcgtg tctgcaagtc cgccaacttt
    22141 ggccacgacg ggcacggcac catattgtag cgcgcacaat tgggtcaggc cgcaaggctc
    22201 atatcgcgag gggacaagca tggcatcggc ccccgcctgc atgagatgag cgagatcttc
    22261 gttgtagccg acgatgcacc cgacgcggcc gggatgagat tccacgagcg acaccaggcg
    22321 gcgctccaac gcctgttcac cggttcccag cacgacgagt tgtgctccgg tatcgagaag
    22381 tatcggcaag gcatccgcca aaatgtcgag gcccttctgc caggcaagcc ggctcacgac
    22441 gccaagaagg agccgttccg ggttttcctc cagtgcgaaa cgccgctgca gatgcgcctt
    22501 gttcgcggtg cgctttgaaa gcgtcgtgcg accgaaacgc atggggatgc gcgtgtcccg
    22561 cagggggttc cagacgttca catcgatgcc gttggtgatg cccgtgatca cgtccgaacg
    22621 gccccgcaac aaaccgtcaa gcccgaatcc attggccggc gtctgcatct ccgccgcata
    22681 ggttggcgat accgtcgtga tccggtccgc gaattgcaga ccggccttga gatatccgat
    22741 cttgccgtaa tattcgatgc catcgacctg aaaagcggcg ggcggcaaac gcagcgcgct
    22801 cagcatttcg gcggggaaca agccctgata ggccaaatta tggatggtga cgaccgaacc
    22861 gggcaccggc cttccatcat aggcgagata gccaagcgtg agaccggcct gccaatcgtg
    22921 acaatgcacg atgtcggggc ggaattgcgc aacctcgccg aggccgatcc gtgccgcgac
    22981 ccatccaaga gccgcgaagc gcaaggcatt gtcgggccaa tcgataccgt cggcatcgac
    23041 ataggggttg ccatcacgat cgaaaagatg cggcgcgtcg atcacaagca gatcgtgacc
    23101 ggcggcgtat ccggcgagga ggcgcgcggg gcccccgaaa agatcgtcat cggccaggac
    23161 cgcatgaaca ttttcgagcg ccgccaccac ggcgggataa cccggcacga gggtaaccat
    23221 cctgacgcca tgttcccgca aagcgagagg cagggcgccc gtgacgtctc caaggccacc
    23281 ggtctttatc agcgggaaga tttccgacac gaccgaaaga acactgacgc ctttaacatc
    23341 gctcatgacg ccagcttgtc gatcatggat cgcgtaatca aacagacgcc actctcggta
    23401 cggcggaatc gtctggcgtc cgcgtcggga tcctcaccca cgacgagatt ttcagggatg
    23461 tgcacgccgc gatcgatgat gacgttgcgc aaccgcgcgg cgcgttcgat caccacttcc
    23521 ggcaaaacaa tggcattttc cacgtgggaa taggatttca gccaggcacg agtgaacagc
    23581 agcgatttct tgacggacgc gccggaaacg atgcacccgc cagaaaccag tgacgacacc
    23641 gcctgaccac ggcgtccgtc caaatcatgg acgaatttcg ccggaggggt tatttcggag
    23701 aacgtccaga tcggccaatc ctgatcataa agatcgagct gcggaacgat gtcggtcaaa
    23761 tccatattgg ccgcaaaata cgcatccagc gtaccgacat cgcgccaata ggcatgagtc
    23821 tgaccgggcg tcctcacgca ggaacgcgaa taatgatggg ccacggcttt gccgttccga
    23881 accagatagg gaatgatatc cttgccgaaa tcgcggctgg aattggggtc cgccgcatcg
    23941 cgcctgagct gatcgatcag gaagcgcgtt tcgaagacat agacccccat gctcgcgagc
    24001 gcgtgatcgg acttggtggg catgggcggg gggactttgg gcttttcaat gaaggaaaga
    24061 atgcggtcat tggcgtccac atgcatgatg ccaaagcccg aagcttccgc cagtggaact
    24121 tccagacagc cgatcgttac gtcagcgccc tgttccacat gctgttggaa catcagctcg
    24181 tagtccattt tgtagacatg atcgcccgcc agaacagcaa tgtgcttcgg agcatagctc
    24241 tcgatgatgt cgatattctg atagatggcg tcggcggttc ctgcgtacca gccggtttcc
    24301 gacactctct gactcgccgg caggatgtcg aaactttcat tgcgctccag acggaaaaaa
    24361 ttccatcccc gctgcagatg ccggatgagg ctgtgggcct tgtattgggt ggcgacgccg
    24421 atgcgcttta tccccgaatt gagtgcgttg gagagcgcaa aatcgatgat ccgggatttg
    24481 cacccgaaaa ataccgcagg cttggcccgc caatcggtga gttccagcag cctgctgcct
    24541 cggcctccag ccaggacata tgccatcgcc gaccgtgaat agggtgcagc ggacagggga
    24601 gaggccacga tcgatcaccg cgcaatttgc ttatgcgaca ggctattatt gtccaagatt
    24661 tccgcacaac gcacgcgata tcgaacacgc gcgataataa gcaatttacg tgccagtgcc
    24721 ggcgccgggc cccggtgcct cgaacgggac gttccagatc tctcgcgcgt actcgccgat
    24781 ggcgcggtcg gacgaaaacc aatgcattcg cgcgatattg aagatgctgg cgcgcatcca
    24841 ccgtgctttc gagatccaca attcgtcgac ctgtctctgg gcctgaaaat aggcttcgaa
    24901 gtcggaggcg accatgtagt aatccgaata ccgcagcgcc tgcatcagag ccgtgaaccg
    24961 atgcggttcg tcgggcgaat aaaagcccga ctcgatcgcc gaaatgacat ccgaaagctt
    25021 ggaggatgcg gtgatggccg cgccggcgtc caacccgtag cgccgccgtt cttcgacttc
    25081 gttcgccttc aagccgaaaa tgaagatgtt ttcatcgtcg acctgttcgc gaatttcaat
    25141 gttggcgcca tccagcgtgc cgatggtcaa ggcgccattc aacgcgagct tcatgtttcc
    25201 ggtgccggat gcctccatgc cggcggtcga gatctgttcg gagaggtcgg ctgccggaat
    25261 gatcgcttcg gccatgctca cattgtagtc gggcagatag gcgacgctca gcagattgcg
    25321 aatcgacgca tcgccgttga tcgtcttgcc aacatcgttg atgagcctga tgatcagctt
    25381 tgcctgggtg taactcggcg ctgccttgcc ggcaaaaatc ttcacacggg gaacccaatt
    25441 cccggaggga ttggcgcgga tcgcgtcata catcgccacc gtttccagaa gattgagcaa
    25501 ttgacgcttg tattcgtgca cgcgcttgat ctgcacgtcg aatatcgcat tcgggtccac
    25561 ccgcaagccc aaacgattgc cgatcaaccg tgaaagcgcc atcttgttgc ggtgcttggc
    25621 ttcgccgatc aatctctgca gcgtgggatc gtccgcgcga tcggcgagcc gcgcgagata
    25681 ggccggattg tcgagaacca cgtcgccgca ggtttttcgc acaatttcca agagttccgg
    25741 attggcttgc ataagccagc ggcggaacgt gatgccgttg gttttgttga cgatccgttc
    25801 gggatagagc gtgtgaagat cgcgaaacac ggtcttcttc atcaggtcgg tatgaagcgc
    25861 cgacacgccg ttcacatgat gggagccgac gaatgcgagg tgccccatgc gcactctccg
    25921 gccgccatat tcatcgatca acgacaccgc cgagagaaga tcgggagaga aagcagcatt
    25981 gcgtttggcc tgctcaaggt gctcggcatt gatgcgatag atgatctgca tgtgccgcgg
    26041 cagcatggtt tcgaagacgg aaaccggcca actttcgagc gcttcgggta gcagggtatg
    26101 gttggtatag gaaagcgtct ttacggcgac ttgccatgcg tcgcgccacg gcacgccgtg
    26161 cacgtcgagc aggatgcgca aaagttcggc aacggcaagg ctcggatggg tgtcgttcaa
    26221 ctggatcgcc gcgcgcgacg gcaattgaag aaggtcccca taggtcttga ggtggcgttg
    26281 caccagatcc tgcaaggagg cggatacgaa gaaatattcc tgccgcagcc ggagttcgcg
    26341 gccagcctcc gtcgcgtcgc tcggatagag gaatttcgaa atcgcctcgg cgcgcgccaa
    26401 gtccgaaaat gctccgaaat ggtccccgct attgaacgca tcgaggcgca acggatcggc
    26461 cgcgcgcgcc gaccagagac gcagcggatt gacgtggccc ccgcgccacc cgacgattgg
    26521 aatgtcgtac gccacagctt ggatcgtctc ggcaggatgc cagatggacc gcacggcgcc
    26581 gcgcggccac gccgccgttt ccacacggcc cccgaaacca atgtcatagg tgacgtcagg
    26641 acgcgcgaat tcccacggat ttccaaatga aagccactct tcgggatatt cctgctggat
    26701 tccgtcgcga atgacttgcc ggaacaatcc atgctggtag cggataccat agccgcaggc
    26761 cgggatgccg agcgttgcca tgctctccat gaagcaggcg gcgagccggc cgagacctcc
    26821 attgccaagc gcggcgtccg gttcggccgc cctcaggacg ccgagatcga ctcccaggtc
    26881 gcccaaagcg gcgcggaaca attccatcaa tcccaggttg cacaagacat cggcaagaag
    26941 ccggccgatc aggaactcca gggacaggta gtaaacgcgc ttggagccct tcgcctggct
    27001 ctcgcgatcg ctggcgagcc aacgatgcat aacgcggtcc cgaatggcga gcgccgtcgc
    27061 cacgaaccag tcgcggtccg aggccgaagc cgcatccttg cccacggtga gcgccaattt
    27121 ggccagcacc gcgcttttga cttcctcgat ctcggcatcg ctgggaggac cggaaggcga
    27181 aaaacccgtc tcatgcgatc cggatggttc cgaacgtcct tcaccttgac ccatgctacg
    27241 cccccatccc ggcccctgtc tcaggcgcca ccctagagtg gaactccatg gcgcgagcta
    27301 cgtcaattcg cacaggagga gaaatgactt cctcacggca aaaggataac ccgcgcggga
    27361 acgctcaaat ccacggcagt tcgggctatg ccaccggcac cggccttgct ggcggtcgaa
    27421 gagatccgtc aatgatgccg tttctgcaaa agtgcagcgt tctggaattt acgattttgc
    27481 ggctttgaaa ggtccagcca tgaacgccgt acggaaccgc acaagctgtt cgcgtgtgga
    27541 aaatggccgg cgccaaacga cggcatctga acaccccgat gtcgcggcgg aactgcgaag
    27601 accgtgagcg aaaattccgg aaccgttgcc gatgaatcag gctcgaattc ccgctggtgt
    27661 gcgaaggagg cataatccta atatgccgcc agcaatgggt gtgcgaccct tatgcggtgt
    27721 tattggggga gatttgattg ccaacagaag gggggatacc cgttatacca ttggcggaat
    27781 tggaaaagga cgccctgacc gaaatcgcga acatcgccat ggggcgcgcg gccaccagtc
    27841 tggggcagat ggtaaacgga gaagtccgtc ttaccgtgcc gtccatagac atcgtgccga
    27901 acgagcgggc atcgatgatc gtggcaaagt ccgacgatct gaggcttatc gccgtacgtc
    27961 aggatttccg tggcttgttt tcaggccgtg ccattctgat ttttccggaa gcgagcagtc
    28021 tcgatctcat ccagatcatc gtcggccggc agctatcgca cgaggatatc ctcgatctgg
    28081 aagaagaggc actggcagaa accggaaaca tcatcttgaa cagctgggtt gccacgctgg
    28141 caaacctgct gaagcacaat cttacgatgt cgcttcccat cgtggtgcat ggcaacagca
    28201 caaagatgtt tgagggcacg ggagaaagcg cacagctcgt gctcttcctg cacatcaatt
    28261 tttccgtaag cgaaaccacg atatcgggct atctggcgct gcttatggat atcccctcga
    28321 tggatgcgct gcgcgcgctc attgccgact atatcagcag tttctgacgc ttcatcgcga
    28381 gtcggccagc atctgccgta tcttgagcgc cagcgcatcg agcatgaagg gcttggtcac
    28441 catctccatt ccctgttcca aaaaggggtg tttgccggtg gcgtgttcgg catagccggt
    28501 gacaaagagg attttcagac tcggacgatg ctgtcgcgcg atctcggcga gttcacgtcc
    28561 attcatgccc ggcaaaccga catcggtgat catgagatcc agccgcgcgt tggacgtcag
    28621 catcggcagc gccgcctggc catcactggc ttcgatgcat gcgtagccga gatcgcgcag
    28681 gacttcaccg ataaggagac gaaccgaaga gtcgtcctct acgagaagaa ccgcctcacc
    28741 cttgccaagc ggcgcgtcac gcgtatccaa aagaaaatcg ggcatcagcg ggcccatata
    28801 gcggggaaga tacaagctcg cctgcgtccc ccttccgagg gtgctttcga tccggatatg
    28861 gccgcgggat tgcttggcga agccatagat catcgaaagt ccaagcccgg ttccttgtcc
    28921 gatcggtttg gtggtgaaga acggttcgaa gatcttgggc accacctccg gtgacatgcc
    28981 tacacccgta tcgctcacgg cgatgacggt gtagtcgccc ggatctattt cgtcctgtcc
    29041 atggtgctcc gcttcggcga ccaggatatt tctcgtcgcg atcgtcaact gacctccggt
    29101 tggcatggcg tcgcgcgcgt tgatcgcaag attgagaagg gcattttcca actggtgcgc
    29161 atcggtgaat gccggccaca ggtctggagc caaatccgtc accaatttca cttgctcgcc
    29221 cagggtgcgc cgcagcagat cctccattcc gcggacgagc cggttcgcct cgacggcctg
    29281 cggattcaaa ggctgccgcc gagcgaacgc cagcagacga tgcgtcagcg ccgccgcacg
    29341 attggatgaa ctgatggcgg cgtccatgaa gcgcgccaga tcatcatagc gtccgaccgc
    29401 gatacgccgt ttcagagcgt ccatcgcgcc aatgatgcca gtcagaagat tgttgaaatc
    29461 gtgagcgatc ccacccgtga gctgtccgat cgcctccatc ttttgcgctt gtcgcaaagc
    29521 ttcctccgcg ccgcgcaagg ccttctcggc ctcacgttcc gccgtcacgt cgcggccgac
    29581 cgcctgcagc agattatcgg atgcgacagc gctccattcg atacgccggg tagcccgatt
    29641 gcgcgtcagc agagtcactt cgaacaactt cggagcagaa ctcgcggaca aggccgcccg
    29701 ggcgatctcg aattgcggac ggtcctccgg aacgatgaag gcctcgatgt tggaccccac
    29761 catatcgcct tcttcccagc caagcaggtg atgccacgcc ggattgaccg cattgatcgt
    29821 cccgtcgaga cgggccacca ccatcacatc ggtggaaagg cgccacatgc gatcgcggtc
    29881 ggccgtgcgt tcggctacgc gctcttcgag cgtgcgattc aagcggtgta gtgcttcctc
    29941 ggccgcgcgc cttacattca tatcatggaa aataaccgtg acaaaaacgc gtgaatcgct
    30001 cagccaacgc gacgccgaca cttccagata ggtaacagac ccgtcttttc tctggccaag
    30061 caccctgatc gaccgggagg gaccaccttt gacgacggaa ttccagacat cggaccagat
    30121 cgtccggtcc ttgagaagga caatgacggg acggccgatc aattcctgca tcgtatattg
    30181 gaagtggttc gccgcggccg gattcacgag ctgaataatt ccatcggaat ccagcgtcag
    30241 aatcgcgtcg ggtgcactct cgacgaccgc gtcgtagcgc gcattctgcc gctcccgcag
    30301 aatccgttcg cgatggaccg cgacagtaag atcggcgatc tgcaaaaggc agcggacatg
    30361 aggcctactg cccaacggag tcacggagat gctgtggatc agatattcac cggcacgcgt
    30421 tttcaggggc agcaaggaag gatgcaagga atgggtcaga aaacttgaca tgcccgattc
    30481 gaataccccc gcgatagcgg acatcaaacg gtggggaaac gagtccgcaa acagctcttc
    30541 cagcgttttt gccaaggcct tttgaaccgg aatgccgctc gccgcgacca gccaccggtt
    30601 ccagacgagc acgcgccttt ggccgtccag cagcatgaca ccggtgtcca atgcgtccag
    30661 aatgtcggcc gaaagctgat cttcaaccgc gaacatctgg ctagaactcc gcctgatccg
    30721 tagtccgctc aaggaaattt cgtatcaatc cccgaagaat cttgagcgac ggcatgtcca
    30781 ttaacatgca aatgtaccca cgaatatcat gttcgcgcac tgtgaaatcg atatagagga
    30841 acatcaccac atcctccgac gtgggaagcg aagccatagc gaaaaagtcc gatccgcccc
    30901 cgtgcaggat ttcggggagc gtcattcgca aactgcattt caggagattg gcgatcgtcg
    30961 caagacagcc attgaggatg atatttccgg tttccgcgag cgcttcctgt tccagttcga
    31021 tgatgtcttc aagggacaac tcgcttttgg ttatggctct gacgagttcg agactcttag
    31081 tttggggaaa gatgagcaac gcccgtcccg cgatgctgcc ctcaaaggtc tgttgaaccg
    31141 cgatgaggtt gtcgggttcg cgttcccgca gaagcctgat ggcttcactg cggctgacca
    31201 gcgcgatcat cggtaccgcc agatggacct gctggctcac catttcctga aggcttaccg
    31261 cagcccggct gacgccgatg ttcaccaatt ccgttagggc atcgcgttca agttcgctca
    31321 ggacggtttc tctggtcacc ttccgggagt gcccgagtgc gccaaggcat ccgatagaaa
    31381 atgggcgagc gcttgcgtgg tcaggggttt catcaggaat gccgctccca gttcgcgcgc
    31441 gcgccgcaag atctcatcct ggtgattggc cgagacaacc gccagggcca gtttgggatt
    31501 caatgcgcga aggtcggccg cgaggtccaa accacttttc cccggcatgt tgaaatcgag
    31561 caatgccaca tcgaagggtg tttgacgcgc caatgccaga gcctcatccg catttgtcgc
    31621 gtccatccgt tcgcactccg gataaagcgc gttgagcgcc tttgtcaccg ccatgcgagc
    31681 cagcttgctg tcgtcgacga caagaatcct gaatgtcatc ccacccactc ttatccaaaa
    31741 acgcggcacc gagacattcg atcccgacgt gttgagcccc gatttttcct cttagccccg
    31801 agataacaag ggctgaaccc aaaatgccag gaacccatct ccgggcggtc cgtgcatcgt
    31861 gaccttggca gtcattaaaa cctgaatcac ggccaaatgc gccttgctgc aacggcgcca
    31921 atccacaatg gcgttgggag ccgcaagaag gcgttccagc agtactgcgg cgtcctcaac
    31981 cggacaatcg ccctccagaa caatcgtcgc gctgtccaac aatcgcaccg tcattcgaga
    32041 atatcctgta catccagcac aataaggacc cggccgtctc ccagcaacgt ggttccgctg
    32101 acgccacgca cgcccgcgag cagcccttcc atcggcgtca tcatcacatc gctttgttct
    32161 ccgatgcgat cgacctcgat accgccaacc tgccgcccca tcgcggttat cacgatcttg
    32221 gcgtcccgcc ccgttgctcc cggcaggtcc ggtttgaggg attgcgcgag attgacaacc
    32281 ggaacagtcc ggttccgcag cacgaaagcc cgccccgcac cgataggcac gatgcgatcg
    32341 cgcgggatac gaacggattc caggacggca tccagcggaa tcccaaacac ctggccgccg
    32401 gcttccaccg tcatcacgcg ggtcatcatc acggtgaaag ggaggatgag ccgcaccgcc
    32461 gtaccgtggc cccactgggt ttcgacggtc accttgccgc ccagccgttc ggcggccgcg
    32521 cgcacggcat ccattccgac accgcgcccg gacagtcctg tgatctgcgc cgcagtggag
    32581 aaacccgccg cgaaaatcag ctggacggcc tcttggtcgg acatcgatcg ggcagcctcc
    32641 gcggatgcga caccccgctc aaccgccaca cgcctgagcc gcgccacgtc tatgccggcg
    32701 ccatcgtcac ggacttcgac cagcacgtgc tggccttgcc gcgcagcccg cagatgaatg
    32761 ctcgccagcg caggcttgcc gcgcgataca cgatcgttcg ccggttcgat gccatgatcg
    32821 accgcatttc ggatgatatg gagcaacggc tcgaacagcg cctccacgac tgttttgtcg
    32881 gcttcggtct cctcaccttc gatgatcagt ttcgcgggtt ttccgagact ctcggacaat
    32941 tctcgaacca atcgcgggaa gcgctgaaag acgtgacgaa gcggaagcac gcgtatgctc
    33001 agcaccgagt gctggagttc ggcgacccgc gcttccagca gcgcgtgccc ctccttcagg
    33061 acggccgcga tggtcttcaa gtcgcctccc gcctgcgcca gttgagcggc atgaccgatg
    33121 gcgttcttca cgacggtgag ttcgctggtc aacttcacca gcgcatcgat gcgcgcgaca
    33181 tccacgcgca gcgaacgcgc tgcgatctcc tcacgcggcc gggcgtgcga ttccggcggc
    33241 ggcagcgcgc cggccgcatc cgcgttcagg gccgccttaa gcgcttctga gagaaaccgt
    33301 ggttcatgat ggatctggct ttggagcaac gccgattcga tctgatccgc gtcctcaaaa
    33361 cggtccctat ggcgaagcac attggctcct actcttccgg cacttgccaa tcgcccggca
    33421 aagccgtcct ccaaattctc cgccaagagc tggatttg
//
LOCUS       DASYEG010000105         2973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_466946, whole genome shotgun sequence.
ACCESSION   DASYEG010000105 DASYEG010000000
VERSION     DASYEG010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466946"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1487)
                     /locus_tag="VGM72_04005"
     CDS             complement(108..1487)
                     /locus_tag="VGM72_04005"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4264457.1"
                     /translation="MTANSSEADSQSHVTARGRIGWVLFEWARNPYVLVITVYVYATY
                     FTRDIVGDPVRGQALWAEIQGYAGLAVAALAPFLGAIADAGGRRKPWVASFAILLGLC
                     TAGLWFGKPGGEGIGLAGIGALIGLANLSYDGSMVFHGAMLPSLVPQMRIGRWSGLGY
                     ALGNLAGIVLLVFVLVFIYLPDKPFFGLDRAAHEHERISGPLCALWFAVFSLPFFLWT
                     PDGRATGLPLSEAVRRGFSSVSRTVKSLGHYRNAAAYLAARMIYNDALNVMLAFGGVY
                     AAGVFHWGPVESAVYGIILSVFAALGGFLGGRLADRIGIKPALQLSIGGTMIAALLSL
                     GFAPDRLFFVFPYAAGTRIAALPVFGTAPELFYIATVMGIAVCLVATYSNSRAMMARI
                     APQSRMTEFFGLYALSGEATAFVAPLAVAFATQASGSQQWGMAVIVAFLAAGFIGLTF
                     VQETRATEI"
     gene            complement(1468..1947)
                     /locus_tag="VGM72_04010"
     CDS             complement(1468..1947)
                     /locus_tag="VGM72_04010"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEY4264458.1"
                     /translation="MRVGLTIVLLLLASAGLPVAAAEETKLPYFASMKDATTNMREGP
                     SEDNRVKWVYHRKGLPVEVIATFEVWRRVRDMDGEIGWIHVALLSRERTAVVRGVGLA
                     AVHDKADAGSPLVAEAKPGAVGRLEHCGPVACEVKFDGATGWIDRMRLWGVHDGEQF"
     gene            2048..>2973
                     /locus_tag="VGM72_04015"
     CDS             2048..>2973
                     /locus_tag="VGM72_04015"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEY4264459.1"
                     /translation="MTGTTPVVIVTRKLPDGVERELAARFHAELNASDTPMNAEVLKA
                     AVARAGVLVPTVTDRIGPEIIAAAGPQLKLIANFGVGFNHIDVKAASARGIAVTNTPG
                     VLTDATADIGMTLMLMVTRRAVEGSNLLHAGQFPGWCPTWLMGVSLRGKTLGIVGMGR
                     IGEAMGLRASAFGMAIHYHNRKRVAPETENALGATYWPDLDAMLGAMDIVSIHAPYTP
                     QTYHLFSAERIARMKPSAYLINTARGEIVDEAALADALASGRLAGAGLDVFEHEPKVH
                     PTLLGLTNVALLPHLGSSTIEARTGMGNKVIA"
BASE COUNT          599 a          992 c          925 g          457 t
ORIGIN      
        1 acggcggtca tccagccggg cggttcggtg aaggacgatg cggtgatcag ggcggcggac
       61 gatgcggggc ttgcgatggt cttcaccggc atccgccact tccggcacta gatttccgtt
      121 gcgcgggttt cctgcacgaa cgtcagcccg atgaacccgg ccgccagaaa cgcgacgatc
      181 accgccatgc cccattgctg actgccgctg gcttgcgtgg cgaatgcgac cgcgagcggc
      241 gccacgaagg cggttgcttc gcccgaaagc gcgtagaggc caaagaattc cgtcatccgc
      301 gattgtggcg cgatacgcgc catcatggcg cggctgttgg aataggtcgc aaccaggcag
      361 accgcgatgc ccatcacggt tgcgatgtag aacagttccg gcgcggtgcc gaagaccggc
      421 aacgcggcga ttcgcgttcc cgccgcatag ggaaacacga agaacagccg atccggcgcg
      481 aagccgagcg acagcaaggc cgcgatcatc gtgccgccga tcgaaagctg cagcgcgggt
      541 tttatgccga tgcggtccgc cagacgcccc ccaagaaaac cacccagcgc ggcgaacacc
      601 gaaaggatga tgccgtaaac tgcgctctcg accggccccc agtggaagac accggcggca
      661 tagacgccgc cgaaggccag catcacgttc agcgcatcgt tgtagatcat gcgcgcggcg
      721 agataggcgg cggcattgcg gtaatggccc agacttttca cggtgcgcga caccgatgaa
      781 aagccgcgcc gtacggcttc gctcaacgga agcccggtcg cgcgcccgtc gggcgtccac
      841 agaaagaacg gcaggctgaa gaccgcgaac cacaaggcac agagcggtcc ggaaatgcgc
      901 tcatgctcat gcgcggcacg atccaacccg aagaagggct tgtccggcag atagatgaag
      961 acaagcacga agacgagaag aacgatgccc gccaaattgc ccagcgcata gccgaggccg
     1021 gaccatcgtc cgatgcgcat ctgcggcacc agcgatggca gcatggcgcc gtggaatacc
     1081 atcgagccgt catagctaag attggcgaga ccgatcaatg cgccgatacc cgcgagcccg
     1141 attccctcgc cgcccggctt gccgaaccac agtccggcgg tgcagagccc gagcagaata
     1201 gcgaaggatg cgacccaggg tttgcgccgg cctccggcat cggcgatcgc gcccaggaac
     1261 ggcgccaacg cggcaaccgc gagccccgca tagccctgga tctccgccca cagcgcttgt
     1321 ccgcgcaccg gatcgcctac gatgtcacgc gtaaaatagg tcgcgtaaac atagacggtt
     1381 atgacgagca cgtaggggtt gcgggcccat tcgaacagca cccagccgat ccgcccccgc
     1441 gcggtcacat gcgattgtga atccgcctca gaactgttcg ccgtcatgca cgccccagag
     1501 gcgcatccgg tcgatccagc ctgtggcgcc gtcgaacttc acttcgcagg ccaccgggcc
     1561 acaatgttcc agccgcccga ccgctccagg tttggcctcg gccaccaaag gcgatcccgc
     1621 atcggccttg tcgtgcaccg ccgccagccc aaccccacgc accactgcgg tgcgctcgcg
     1681 cgacagcagc gccacatgga tccagccgat ctcgccatcc atgtcacgca cccgccgcca
     1741 aacctcgaac gtggcgatca cttccaccgg caggcccttg cggtgataga cccatttgac
     1801 gcgattgtcc tcgctgggac cctcacgcat gttcgtggtc gcgtccttca tgctggcgaa
     1861 atagggcagc ttcgtctcct cggcggccgc aaccggcagg cctgccgatg ccagaagcag
     1921 caacacaatc gtaagcccta cacgcatcgc aagcacaacc gccgattcga gacgctctat
     1981 ctttgctccg cgcgagtcgt tctgataagg agaaaaacgc cgcgcgcggg gttaaatcga
     2041 taccatcatg accggaacaa cgcccgtcgt catcgtcacg cgaaaactgc ccgacggtgt
     2101 cgagcgcgag ctcgccgcgc gcttccatgc ggaactcaac gccagcgata cgccgatgaa
     2161 cgcggaagtg ctgaaagcgg ccgtggcgcg cgccggcgtg ctggttccca ccgtcaccga
     2221 ccgcatcggg cctgagatca tcgccgccgc cggaccgcag ttgaagctga tcgcgaattt
     2281 cggcgtcggc ttcaatcata tcgatgtgaa ggcggcatct gcgcgcggga tcgccgtcac
     2341 caacacgccc ggcgtgctga ccgatgcgac cgccgatatc ggcatgacgc tgatgctgat
     2401 ggtgacgcgc cgcgcggtgg aaggcagcaa tctgctgcat gcgggtcaat tcccggggtg
     2461 gtgtcccacc tggctgatgg gggtgagcct gcgcggcaag acgctcggca ttgtcggcat
     2521 gggccgcatc ggcgaggcga tgggattgcg tgccagcgcc ttcggaatgg cgatccatta
     2581 tcacaatcgc aagcgcgttg cgcccgagac cgagaacgcg ctgggggcga cctattggcc
     2641 cgatctcgat gcgatgctgg gcgcgatgga catcgtgtcg atccatgcgc cctacacgcc
     2701 gcagacctat catctgttct cggcggagcg gatcgcacgc atgaagccat cggcctatct
     2761 gatcaacacc gcccgaggcg aaatcgtgga cgaggcggcg ctggccgatg cgctggcttc
     2821 gggaaggctc gccggtgcgg ggctcgacgt gttcgaacat gaacccaagg tccatcccac
     2881 tctgctgggg ctgacgaacg tcgcgctgct gccgcatctg ggctcctcga ccatcgaggc
     2941 gcgcaccggg atgggcaaca aggtgatcgc caa
//
LOCUS       DASYEG010000106        14109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2205603, whole genome shotgun sequence.
ACCESSION   DASYEG010000106 DASYEG010000000
VERSION     DASYEG010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 14109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14109
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2205603"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VGM72_04020"
     CDS             <1..196
                     /locus_tag="VGM72_04020"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEY4264460.1"
                     /translation="FAKTKAERMTNEDPRPSLEERYHDHAGYVQAVKMAAAKIVAAGF
                     LLQADADRLADEAEKSNVLK"
     gene            complement(303..1313)
                     /locus_tag="VGM72_04025"
     CDS             complement(303..1313)
                     /locus_tag="VGM72_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4264461.1"
                     /translation="MRKIWMLTVSGLVAANFIGPAAFAQNAEAEAAAAAASAAAAAGR
                     ANAKVSPNDSLEKICRIGPRSREVDIQKIEPFKVFDNLYHVGPCYVSSWILTTPQGDI
                     MFDTSQEPFVDMIEANIKKIGVNPHDIKYIIINHGHIDHFGGAKRLQEFTGARVVATP
                     EDWKMIEAFAGKPNNRDGGKPTQVPKRDMEVREGDHFDFGDQHLIIHTAPGHTPGNLF
                     IEGLTLHDGAQTYRGVWGGGGGGAPGLAGAEQGVKNAEKVNAMKGIQVYIQTHAWQDP
                     NGYPGGGIHERAKKLANRKPGDPNPFVDPATWDARAKRQLETAKKVLAEEQAKAGATK
                     TN"
     gene            complement(1548..2579)
                     /locus_tag="VGM72_04030"
     CDS             complement(1548..2579)
                     /locus_tag="VGM72_04030"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY4264462.1"
                     /translation="MTSKTKIAGVAVTLLSLAPICVAGAQTPVVVGAATEIPGVVKGG
                     APIQRILQGYKGLDDPIGLRDGTLVFSEPDGLRLHRLNTQTNEVSVLVAQSNESHGVS
                     QDSQGRLISAQAWDGSTRIGVIYPQGSETVLADKYDGKPFSRPNDLIVTRNGGIYFTD
                     PGLTAQQAVELVKRQGGKPLAPRLPPAVYYIPPGQAPVKIEDRLIRPNGVQLNRDETE
                     LYVDDSNGDHVIAWDIGPKGLVENRREFATLQGRSRRDNGLGGIKTFADGMAVDNDDR
                     LYVATGGGIEVLSPQGQHLGIIPVRCPPADCQNIAFSGPTKQTLYIGGAGSLYKVEMI
                     ARGLTTRSK"
     gene            3061..4170
                     /locus_tag="VGM72_04035"
     CDS             3061..4170
                     /locus_tag="VGM72_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEY4264463.1"
                     /translation="MHVKAAVAFEAGKPLEIVDVQLEGPRDGEVLVELKATGICHTDA
                     FTLSGGDPEGRFPVIFGHEGAGIVREVGKGVTSVKPGDHVIPLYTPECRQCEYCLNPK
                     TNLCQAIRTTQGAGVMPDGTSRFSYRGKPVLHYMGCSTFANFTVLPEIAVAKIRADAP
                     FDKVCYIGCGVTTGIGAVINTAKVEPGSRVVVFGLGGIGLNVIQGARMVGASQIVGVD
                     LNPARRALGEKLGMTDFVNPRDVSGDLVAHLVERTGGGADYSFECIGNVNSMRQALEC
                     CHKGWGMSVIIGVAGAGQEISTRPFQLVTGRVWKGTAFGGARGRTDVPKIVDWYMQGK
                     IAIDDLITHTMPLEKINDAFDLMHEGKSIRSVVLY"
     gene            complement(4178..6307)
                     /locus_tag="VGM72_04040"
     CDS             complement(4178..6307)
                     /locus_tag="VGM72_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4264464.1"
                     /translation="MTYAPKKNATPDHLSAWPIRGESALAIAAGIGLGLAAATSAVAG
                     ENIQQPEVVTITARPPDPVGNKAYSTTLLDEQQLQVSDQIDTALRQVPGLSLFRRNTS
                     LSANPTVQGVSLRSIGASGAGRALVTLDGVPQNDPFGGWVIWSSLPSEDIQGAEIVHG
                     AGAGPYGAGALTGVIALTERAGTGGIIDAEGGELDQGRVAGAGNAQFGNVSIGASAMY
                     QSVGGWVPVAPGQAGAADKPLTLNATSASIHGGVEVVPGTQITGRFGFYDERRDTGIA
                     GATSTAKGNTGSITVANAEGPDTIGWRIQGWYRDTNMSNVTSAVGVKRATVTPAGNQY
                     AVPALGWGGNAAVRGNFSWLDWELGTDARFADGESRELFSFVNGQFRSSRFAGGRTFV
                     GGVYAEGASRVDDWLFTAGVRIDDWRNYDGHTIEHSLATGAMTLNSQPADTSGTVPTG
                     RAGVRKDLGDGLYLRSAAYEGFRAPSLNELYRAFRVGNNYTRANSALKPEKLYGVEVG
                     VGDDEGAFTWDATAFWNKLQDGVTNVTIASGPVSFPDVTLPAGGLLIQRQNVGYIRAL
                     GAEGDAQYQFTNVFAVRGGFAITDARVNGQSVTPQLTGKRPASTPRVTLTAGLVANPF
                     KELTLEGDVRYETARYSDDLNTLKLPPVATVDVKASVHFTDRIDLYLAVDNLFNAQVA
                     TSEGADRVFTYDAPRALRIGIAYRYGS"
     gene            complement(6438..7586)
                     /locus_tag="VGM72_04045"
     CDS             complement(6438..7586)
                     /locus_tag="VGM72_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264465.1"
                     /translation="MRLDWKSFTLAGVSVLSLTSVIGLAMGAPAADKPPTYKYDPAWP
                     KELPNKWTMEGITGLFVDKDDHIWVLNRPRDFDERENAAAVNPPAGECCVPPPAVMEF
                     DTAGNLLTAWGVPNSVPGWPKSEHTIFTDRAGNVYIGGAQAGDSLLKFSHDGKLLSDF
                     GHRGPIVPAGTQQKQDNQQTDLLLRGVAAATLDEDAHEIYIADGYLNRRVLVYDWDTG
                     KFKRGWGAYGKPLAEIDNGPQPPHDPKGPPAKDFKSPVHCVRIAKDGLVYVCDRAGDR
                     IQVFTKEGKYLKEFFIANETLRGGSAGSVDFSSDPGQKYIFVADIVNNVVWELERDTG
                     KVVAKIGHHGHQGGMFHFVHVAFMDSKGNLYTGEVDSGKRVQKFTPVP"
     gene            7665..8546
                     /locus_tag="VGM72_04050"
     CDS             7665..8546
                     /locus_tag="VGM72_04050"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEY4264466.1"
                     /translation="MTLRTPAPSDAEAAHVLAMYAAEGRDAAPDWEGALDHLQQAAEL
                     GHGLAQAELAALCGDWALAQKFGAGKSLTEGTWGDLRRAVDLQRLLTPPRANILSASP
                     RIAAIEAMVAPELCDWLIARARIKLAPAEVYDHETGSQRIEAVRTNSAAYFLWEDSDF
                     LLLALRARMAAAAELPVYAMESTAVLHYTAGQEFMPHFDFLDVAKPGHAKDVAGRGQR
                     VLTFLIALNEGYEGGQTEFPTLGKRWKGRKGSALFFWNVEPDGSVDRRMLHAGLPPTR
                     GEKWLLSQWIRGRPIQS"
     gene            complement(8547..9014)
                     /locus_tag="VGM72_04055"
     CDS             complement(8547..9014)
                     /locus_tag="VGM72_04055"
                     /inference="COORDINATES: protein motif:HMM:NF018748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY4264467.1"
                     /translation="MNTRPPLPPFTSETAAQKARGAEDAWNSRDPKRVALAYTQDSRW
                     RNRAEFFQGREKIVEFLTRKWTKEIEYRLIKEVWAFHANRIAVRFQYEWHDEHGDWFR
                     SHGNEQWEFDEVGLMRRREASINDVPITQAERKFHWPLGPRPADHPGLSEHGL"
     gene            9163..10347
                     /locus_tag="VGM72_04060"
     CDS             9163..10347
                     /locus_tag="VGM72_04060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEY4264468.1"
                     /translation="MSKRPIIRLKPREGRRVRAGAPWVFSNEMTMDERAKALAPGTLV
                     GLATEDGTVLGTGYFNPRSLIGVRMLAGADAVIDTDFFIEKFRRALVLRERFHGAPFY
                     RLVHAEGDGLPGLVIDRFGDACVVQIATAGMERLKDPMLDALDAVIAPVSVILRNDVP
                     SRALEGLDSHVETVRGEAPPRLAVAENGVTYFADPIGGQKSGWYYDQRENRVFMADLA
                     NTARVLDAYCYSGGFGVLAAARGATEIVGLDSSEPALKLAAEGAAANGLSERCRFVKC
                     DVFEELERRARDGELFDIVICDPPPFAPSRKDVEAAARAYRKLARAAAGIVAPGGFLM
                     LASCSHNMSAERFAAECAAGLARSGRRAALIREAGAGPDHPVHPMLPETAYLKTLTYS
                     VG"
     gene            complement(10344..12263)
                     /locus_tag="VGM72_04065"
     CDS             complement(10344..12263)
                     /locus_tag="VGM72_04065"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEY4264469.1"
                     /translation="MQTTEAARGHGGASRVSGLAWRKRQAWLTLGALGVVYGDIGTSP
                     LYAVRQSVLATGGQMPMSSAVLGAISLIFWSLIIIVTIKYIVFILRADNKGEGGVIAL
                     ASLAHRSAGLSRRMKALIGFAGVLGLALFFGDGMLTPAISVLSAVEGLKIEDPRFGPL
                     VLPITLVILLGLFFIQNRGTGKLGRLFGPIVLVWFVVLGLLGLRSILMTPEILFALNP
                     WDGIRLFVMEPWTAFVALGSVVLTVTGVETLYADMGHFGRTAIRLGWLAVTMPGLILN
                     YLGQGAVLLRDPSALDSPFYALAPPGFHYPLVALATVATVIASQAVISGVFSITRQSV
                     QLGQLPRMEIRHTSATDYGQIYVPRANTLMLIGVVAIVLIYKNSDALAAAYGMAVTGM
                     MSITTFLGLIVVIRQWHWKPAWIATAFGVFILTDLTFLGAVSLKLVEGAWLPLAIAAG
                     VFVIMEVWRAGRRVLLEKAYGGGISTDRFLERADKSPIRVAGTAIFVTPRIDEVPGSL
                     LHNLKHNQVLHERVIFLRVDVQDVPFVPPEKRLTVKKLGKGFYTVEVHYGFFQTPDVP
                     AALEGSRAYGLSLDLDATTFFIRRETLVPARASSMRRWQTKLFIKLYASALEAAQFYR
                     LPPGRVVELGSQTEI"
     gene            complement(12485..13615)
                     /locus_tag="VGM72_04070"
     CDS             complement(12485..13615)
                     /locus_tag="VGM72_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4264470.1"
                     /translation="MRMFSFARLYAILVKEFIQMRRDHMTFAIMVGVPILQLLTFGFA
                     INMNPRHLPAAIHVADDSRYARSIAAALKNSEYFDIVAVARSPEEGDRMLDRGDVTFV
                     ITIPVNFARDMIRGLNPEVLIQADATDPVAAGNALAAMTQLATSALKDDLVGPLAIRA
                     AVLPFTAVIQRRYNPEGITQYNIVPGLIAVILTMTMVMVTAMAMTRERERGTLENLLA
                     MPAHPFEVMLGKIIPYVVVGYIQATLILLGARFIFHVPMIGSLLLLVGATGIFIAANL
                     ALGFTFSTVARNQLQAMQMTVFFLLPSILLSGFMFPFRGMPDWAQWIGEALPNTHFLQ
                     IVRGILLKGNGWTEIWPNIWPLLIFLGGVGAIALLRYRRTLD"
     gene            complement(13659..>14109)
                     /locus_tag="VGM72_04075"
     CDS             complement(13659..>14109)
                     /locus_tag="VGM72_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264471.1"
                     /translation="LDEPTAGVDPQARREFWEQIHALSQSGLTVLVSTHYMDEAERCD
                     RIVYINQGRIIARGTVDDVIKQSGLVTFVIEGRDVRRLLPQLQGKPGVEHVAFFGAAL
                     HASGHDRTLLEQTVAALRGQSGVEMHEETPSLEDVFIHLQGTHIRAQ"
BASE COUNT         2694 a         4292 c         4410 g         2713 t
ORIGIN      
        1 gttcgccaag accaaggcgg agcgcatgac caatgaggac ccgcgtccct cgctggagga
       61 gcgctatcac gatcatgcgg gctatgttca ggcggtgaag atggcagcgg cgaaaatcgt
      121 ggcggccggg ttcctgttgc aagccgatgc ggaccgtctg gcggacgaag ccgaaaagag
      181 caacgtcctg aaataggcgt tcgatcgcat tctcgcctcc caaatcaggg gaaaatgcga
      241 tctcacgacg ggccccggtc ctggccgcgc ttagctacgt ctgccttgcc tcgaaactcc
      301 ggtcaattcg tcttggtggc gcccgccttg gcctgctctt cggccagcac tttcttggcc
      361 gtttcaagct ggcgcttggc gcgcgcgtcc caggtcgccg gatcgacgaa gggattcggg
      421 tcgcccggct tgcggttggc gagcttcttg gcgcgctcat ggatgccgcc gcccggatag
      481 ccgttcgggt cctgccaggc atgggtctgg atatagacct ggatgccctt catggcatta
      541 accttctctg cgttcttcac cccttgctcg gcacccgcaa ggcccggcgc accgcccccg
      601 ccgccccccc agacgccgcg ataggtctgg gcgccgtcat gcagggtaag gccctcgatg
      661 aacaaattgc ccggcgtgtg gcctggggcg gtgtggatga tgaggtgctg atcgccgaag
      721 tcgaaatgat cgccctcacg cacttccatg tcgcgcttgg gcacttgggt aggcttgccg
      781 ccgtcgcggt tgttgggctt accggcaaag gcttcgatca tcttccagtc ttcgggcgtc
      841 gccacgacgc gcgcgccggt gaattcctgc agacgcttcg cgccgccgaa atggtcgata
      901 tggccatggt tgatgatgat gtatttgatg tcgtgcgggt tgacgccaat cttcttgatg
      961 ttcgcctcga tcatgtcgac aaagggctcc tgcgacgtat cgaacatgat gtcgccttgc
     1021 ggcgtcgtca ggatccagga cgacacgtag cagggaccga catggtagag attgtcgaag
     1081 accttgaagg gctctatctt ctggatatcg acttcgcgcg agcgcgggcc gatgcggcag
     1141 atcttctcga gcgaatcatt gggcgaaacc ttcgcgttgg cgcggcctgc ggcggcggca
     1201 gcggaggcgg cggcagcagc ggcttccgct tcggcgttct gcgcaaaggc cgcaggtccg
     1261 ataaaattgg cggccacgag gccgctcacc gtcagcatcc agatttttct cattgcatcc
     1321 tcccgtggcc cgtgccgggc gcatttaatt tcgcaacgct cgccagtcta tcgcgttgcc
     1381 gcccgccacg gaaagggtgc ggcaccccga ttttctacgc cgtgagcagc agcatagcgt
     1441 tcttggtccg ggttgccggc gtagtggcgc gctgcaaccc tggccgtcgc cgagacaaat
     1501 ttcatcgtcg atcaagcgtg caggatcttg gcgtcccggc tgccgctcta ttttgagcgc
     1561 gtcgtaaggc cccgagcgat catctcgacc ttatagagag agcctgcccc gccgatatac
     1621 agcgtctgct tggtgggccc gctgaatgcg atattctgac aatctgccgg cggacagcgc
     1681 acgggtatga tgccgaggtg ctgcccttgc ggactcaaga cctcgatacc gcctccggtg
     1741 gcgacgtaga gccggtcgtc attgtcgaca gccatgccgt ccgcgaacgt cttgatgccg
     1801 cccagaccgt tgtcccggcg gcttcgaccc tgcagagtgg cgaactcgcg ccggttttcg
     1861 acaaggccct tcggcccgat atcccaagcg atgacgtggt ccccattgga atcgtcaacg
     1921 tacagctcgg tttcgtctcg attgagctga acgccgtttg gccggatcaa tctgtcttcg
     1981 atcttcaccg gcgcttgccc aggcgggatg tagtacacgg cgggcggcag gcgcggggca
     2041 aggggtttgc cgccttgcct cttcaccaat tccacggcct gctgcgcggt gagcccgggg
     2101 tccgtgaagt agatgccgcc attgcgggtg acgatcaggt cgttcggacg actgaacggc
     2161 ttgccgtcgt acttgtcagc gaggacggtt tcactcccct gcggatagat cacaccgatc
     2221 ctcgtggacc catcccaggc ttgggccgag atcaaccgtc cctgggagtc ctggcttacg
     2281 ccgtgcgatt cattggattg cgcaacgagg accgaaacct cgttggtctg cgtgttcaag
     2341 cggtgcaggc ggagcccatc cggttcggag aacaccagcg ttccgtccct cagcccaatc
     2401 ggatcgtcca ggcctttata gccttggaga atgcgttgaa tcggggctcc gcccttgacc
     2461 acaccgggga tttccgttgc ggcgccgacg acgaccggtg tctgagcgcc agcgacgcaa
     2521 atgggtgcca gcgacagtag cgtgactgcg acgcccgcga tttttgtttt tgatgtcatg
     2581 tcgcgattaa ctccagaacg aaaatgggag cactccgccg tcggtgggag aacaacctaa
     2641 catagacttg caagccagcc atgggcggcg aggcacggca agaatatgcg cgctgctcga
     2701 tgcccccaat tgcgatgaat aacggaccgt gcggcgacct gcctcgaacg tctgctttgg
     2761 cctaaaggac gtcgcactca tccattcgaa gcggcacatt tccagaatcc cacggcgatc
     2821 ccaattttgt tcaacgcttg cgcgtttcaa tgcgccggca ccgtccccat atgccatagc
     2881 caagctaagg gaaggagcag gtcgctgtgt gcccgcaatt gcaccggaat tcgcaccaaa
     2941 taatcttgcg cgaggtccgg tttttcttaa gggttccctg gagttctctc gttcgaaccc
     3001 tcaaattcgg ggggagggga ggagtgcccc tttttgcgaa tttttgtccg gagataatac
     3061 atgcacgtga aagccgcggt agcgttcgag gcgggcaagc cgcttgaaat cgtcgatgta
     3121 caactggagg gtccccgcga cggcgaagtg ctggtcgagc tgaaggccac cggcatctgc
     3181 cacaccgacg cattcacgct ttcgggcggc gatccggaag ggcgctttcc cgtcatcttc
     3241 ggtcatgagg gtgcgggcat cgtgcgcgaa gtgggcaagg gtgtgacctc cgtcaaaccc
     3301 ggcgatcacg tgattcctct gtacacgccc gaatgccggc aatgcgaata ttgcctcaac
     3361 ccgaagacca atctttgcca ggcgatccgc accacccaag gcgcgggtgt gatgccggac
     3421 ggcacgagcc gcttttccta tcgcggcaag ccggtgctgc attacatggg gtgttcgacc
     3481 ttcgccaatt tcaccgtgct gccggagatc gcggtggcga aaatccgcgc cgacgcgccg
     3541 ttcgacaagg tttgctatat cggctgcggc gtcaccacgg ggatcggcgc cgtcatcaac
     3601 accgcgaagg tggaaccggg ctcgcgcgtg gtggtgttcg ggctcggcgg catcgggctc
     3661 aatgtgatcc agggtgcgcg catggtaggt gcctcccaga tcgtcggcgt cgacctcaat
     3721 ccggcgcgcc gagcgcttgg cgaaaaattg ggcatgaccg atttcgtcaa tccgcgcgat
     3781 gtctcaggcg atctggtggc gcatctggtc gagcggacgg gcggcggcgc cgattacagc
     3841 ttcgaatgca tcggcaatgt gaactccatg cgccaagcgc tcgaatgctg ccacaagggc
     3901 tggggcatga gcgtcatcat cggcgtggcg ggcgcgggcc aggaaatctc cacgcgcccc
     3961 tttcagctcg tgacgggccg cgtctggaag ggcaccgcgt tcggcggcgc gagaggccga
     4021 accgatgtgc caaaaatcgt cgactggtac atgcagggga aaatcgccat cgacgatctc
     4081 atcacccaca ccatgccgct agaaaagatc aacgatgcct tcgacctgat gcacgaaggc
     4141 aagtctatcc gctcggtggt cctctactga gatgcggtca cgatccgtaa cgataggcga
     4201 tgccgatccg gagcgcgcgc ggtgcatcat aggtgaagac tctgtcggca ccttccgaag
     4261 tcgcaacctg cgcgttgaac agattgtcca cggccaaata gagatcgatc cggtcggtga
     4321 aatgcacgct cgccttcacg tctaccgtcg caacgggcgg gagcttcagc gtgttcagat
     4381 cgtcggaata gcgcgccgtc tcgtaacgca cgtcaccctc cagcgtcagt tccttgaacg
     4441 ggttcgcaac caggcccgcg gtcagcgtca cacgcggcgt cgaagctggg cgcttgcccg
     4501 tgagctgcgg tgtgaccgat tggccattga cccgcgcatc ggtgatcgcg aatccgccgc
     4561 gcaccgcgaa cacattggtg aactggtatt gcgcgtcgcc ttccgcaccc agagcgcgga
     4621 tgtaacccac gttctggcgc tgaatcagca gcccgccggc gggcaaggtc acatccggaa
     4681 acgagaccgg tccgctggcg attgtgacgt tggtcacccc atcttggagc ttgttccaga
     4741 atgcggtcgc atcccaggtg aaagcgcctt catcgtcgcc cacaccgact tccacgccgt
     4801 aaagcttctc cggcttcagg gcggaattgg cgcgtgtgta attgttgccg acgcggaagg
     4861 cgcgataaag ctcgttcagc gaaggggcgc gaaagccttc ataggcggcg gagcgaagat
     4921 aaagcccatc acccaaatcc ttgcgcacgc ccgcgcgccc ggtgggaacc gtaccgctgg
     4981 tgtcggcggg ctggctgttg agagtcattg cgccggtcgc aaggctgtgc tcgatcgtgt
     5041 ggccgtcgta attcctccaa tcgtcgatgc gcactccggc tgtgaacagc caatcgtcca
     5101 ccctgctggc gccttcggca tagacgcccc cgacaaaggt gcgtccgccg gcgaagcggc
     5161 tggagcggaa ttgaccgttg acgaagctga aaagctcgcg cgattcccca tcggcgaatc
     5221 gtgcatccgt gcccagttcc caatccagcc aggagaaatt cccgcgcacc gcagcgttgc
     5281 cgccccagcc gagcgcgggc accgcatatt ggtttccggc gggcgtgacc gtcgcgcgtt
     5341 tgaccccgac cgcggacgtc acattggaca tgttggtatc ccggtaccaa ccctgaatgc
     5401 gccagccgat tgtatccggt ccttcggcat tggcgaccgt gatgcttccg gtgttgccct
     5461 tcgccgttga cgtggctccg gcaatgccgg tatcgcggcg ctcgtcgtaa aagccgaaac
     5521 gtccggtaat ttgtgtcccc ggcacgactt caacgccgcc gtggatggac gcgctcgtgg
     5581 cattcagtgt cagcggtttg tcggcggcgc ccgcttgacc gggggcaacc ggcacccagc
     5641 cgcctacgga ctgatacatg gcgctggcgc cgatgctcac attgccgaat tgggcatttc
     5701 ctgcaccggc gacgcgtccc tggtccaatt cgccgccttc ggcgtcgatg atacccccgg
     5761 tgcccgctct ttcggtcagc gcgatgacgc cggtcagcgc acccgcgcca tagggcccgg
     5821 cgccggcgcc atggacgatc tctgccccct gaatatcttc cgatggcagc gaactccaga
     5881 tcacccagcc gccaaaagga tcgttctgcg gcacgccatc cagtgtgacc agcgcacgtc
     5941 ccgcgccgga ggcgccgatg gagcgcaggg acacgccctg tacggtgggg ttggccgaaa
     6001 gactcgtatt gcgccggaac agcgacaggc cgggcacctg acgcagcgcc gtatcgatct
     6061 ggtccgacac ctgcaattgc tgctcatcaa gaagcgtcgt ggaataggct ttgttgccaa
     6121 ccggatcggg aggccgtgcg gtgatcgtca cgacctcagg ctgctggata ttttcaccag
     6181 cgaccgcgga cgtcgccgca gcgagaccca gaccgatacc ggctgcgatg gcgagcgcgg
     6241 actcgccgcg tatgggccat gcggagagat gatccggtgt ggcgttcttt ttcggcgcat
     6301 atgtcatctt gtgcctccca cggcgcaaat tcgcttattt cgcgccgctt cgccactggt
     6361 ccgtgggaaa atccgcgcac tgacaattgg cagccttgag cgaaagaacg ctttccggct
     6421 tgtgcaatga tgatgagcta tgggaccggc gtgaatttct ggacccgttt tcccgaatcc
     6481 acctcgcccg tgtagagatt gcccttcgaa tccatgaacg ccacatggac gaaatggaac
     6541 attccgccct gatggccgtg atggccgatc ttggccacga ccttgccggt gtcgcgttcc
     6601 aattcccaga cgacgttgtt cacgatgtcg gcaacgaaaa tatatttttg gccaggatcg
     6661 gaagagaaat cgaccgagcc cgccgagccg ccgcgcaagg tctcgttggc gatgaagaat
     6721 tccttcagat acttcccctc tttggtgaag acctgaatgc ggtctccggc gcggtcgcag
     6781 acatagacga gcccgtcctt cgcgatacgc acgcaatgca ccggcgattt gaaatcctta
     6841 gcgggcggtc ccttgggatc gtgcggcggt tgcgggccgt tatcgatctc cgcaagcggc
     6901 ttgccgtagg cgccccagcc gcgtttgaac ttgccggtat cccaatcata gaccagcacg
     6961 cgccgattga gataaccgtc ggcgatatag atttcatggg cgtcttcgtc gagggttgcc
     7021 gccgcaacgc cgcgcaggag cagatcggtc tgctgattgt cctgtttttg ttgcgtccct
     7081 gccggcacga tggggccgcg gtggccgaaa tcggacagca gctttccgtc atggctgaat
     7141 ttgagcagcg aatcgcctgc ctgggcgccg ccgatataca cattgccggc gcgatcggtg
     7201 aaaatcgtat gctccgattt cggccagccc ggaacggaat tgggcacgcc ccaggcggtc
     7261 agaagatttc ccgccgtatc gaactccatc accgcaggcg gcggcacaca gcactcgccg
     7321 gccggcggat tgaccgcggc ggcgttttcg cgttcgtcga aatcacgcgg acggttcagc
     7381 acccagatgt gatcgtcctt gtcgacgaac agcccggtaa tgccttccat ggtccatttg
     7441 ttggggagtt ccttgggcca ggccggatcg tatttgtagg tcggcggttt gtccgccgcg
     7501 ggcgcgccca tagccaagcc gatgacgctc gtcagcgaca gcacagaaac ccccgcgagc
     7561 gtaaaacttt tccaatccag gcgcatgcgt gtcctcccca cgaaagccga acaaacgtca
     7621 gattatcagc agcaagacgg aagggatact gttagcattt gcccatgaca ttgcgcaccc
     7681 cagcaccgag cgacgccgaa gctgcccatg tcctggcgat gtatgcggcc gaaggcaggg
     7741 acgctgctcc cgattgggag ggtgcgctcg atcatttgca gcaggcagcc gaactcggac
     7801 atggtctcgc gcaagccgaa cttgcggcac tctgcggcga ttgggccttg gcgcaaaagt
     7861 tcggcgcggg aaaatcgtta accgaaggca catggggaga tttgcggcgc gcggtcgatc
     7921 ttcagcggtt gctcacgccg ccgcgcgcca acattctctc cgcgagcccg cggatcgcgg
     7981 cgatcgaagc catggtggca ccggaactct gcgattggtt gatcgcgcgc gcgcgcatca
     8041 agcttgcgcc ggctgaggtc tacgatcacg agacaggcag tcaaaggatc gaagcggtac
     8101 gcaccaacag cgcggcctat tttctgtggg aggacagcga ttttttgctg ctcgccttgc
     8161 gcgcgcggat ggcggcggcc gccgaattgc cggtttacgc catggaatcg acggcagtcc
     8221 tccattacac ggccgggcag gaattcatgc cccatttcga ctttctcgat gtcgctaaac
     8281 ccggtcacgc caaggatgtg gcagggcgcg gccagcgggt gctgacattt ctgatcgcgc
     8341 tgaatgaggg ttacgagggc ggccagaccg aattcccgac gctcggaaaa cgctggaaag
     8401 ggcgcaaggg cagcgcgctg ttcttttgga atgtggaacc cgacggcagt gtcgaccggc
     8461 ggatgcttca cgcaggcctt ccccccacac ggggcgagaa atggctgctc tcgcaatgga
     8521 tccgcggccg tcccatccaa agctgattaa agtccgtgtt cgctcaatcc cggatggtct
     8581 gcaggacgcg ggccgagcgg ccagtggaat tttctctcgg cttgcgtgat cggcacatcg
     8641 ttgatgctgg cttcgcgccg gcgcatcaga cccacctcgt cgaattccca ttgctcattg
     8701 ccgtggctgc gaaaccaatc gccatgttcg tcatgccatt catattggaa acgcacggcg
     8761 atgcggttgg cgtgaaaggc ccagacctcc ttgatgagac ggtactcgat ttccttcgtc
     8821 catttgcgcg tcaggaattc gacgattttt tcgcgcccct gaaagaactc ggcgcggtta
     8881 cgccagcggc tgtcctgggt ataggccagc gcgacgcgtt tggggtcgcg gctattccat
     8941 gcgtcctcgg ccccgcgggc tttttgcgcg gcggtttcgg acgtgaaggg tggcaaaggc
     9001 ggtcgggtgt tcatagccgt aaatcagctt tctttgcgat ggggatgggg tttccatatg
     9061 ccgggccgga cacgatcttt caggttgttg cgtcttgttc gcttcccacg cactccggtc
     9121 ctctatatca aagcgcaaac atccgaagca aacggcaagc tcttgagcaa acgtcccatt
     9181 atccgcttaa agccgcgcga gggccgccgc gtgcgcgccg gcgcgccatg ggtcttctcc
     9241 aatgagatga cgatggatga gcgcgccaag gcgctcgcgc ccggcacgct ggtcggattg
     9301 gcaaccgagg atggcacggt gctgggcacc ggctatttca atccgcgaag cctgatcggc
     9361 gttcgcatgc tcgcgggtgc ggatgcggtt atcgatacgg atttcttcat cgaaaaattc
     9421 cgccgggcgc tggttttgcg cgaacggttc catggcgcgc ccttctacag gctggtgcat
     9481 gcggaaggcg atggtctgcc ggggcttgtg atcgaccgtt tcggcgatgc ctgtgtggtg
     9541 cagatcgcca ctgcgggcat ggaacgcctg aaagatccga tgctggacgc gctcgatgcg
     9601 gtgattgcgc cagtctcggt gatcctgcgc aacgatgtgc cgtcgcgtgc gctcgaaggt
     9661 ttggacagcc atgtcgagac cgtacgcggt gaagccccgc cgcgattggc ggtcgcggaa
     9721 aacggcgtca cctatttcgc cgatcccatc ggcgggcaga aatccgggtg gtattacgat
     9781 cagcgggaaa atcgcgtctt catggcggac ctcgcgaaca ctgcgcgcgt gctggatgcg
     9841 tattgctaca gcggcgggtt cggcgtgctc gccgcggcgc gcggagccac ggagatcgtc
     9901 ggtctggatt cgtcggaacc ggcgttgaag ctcgctgccg aaggcgccgc cgcgaacggg
     9961 ctgagcgaac ggtgccgttt cgtgaaatgc gatgtgttcg aggaattgga gcggcgtgcg
    10021 cgagacgggg aacttttcga catcgtgatc tgcgatccgc cgcccttcgc gccctcgcgc
    10081 aaggatgtcg aagcggcggc gcgcgcctac cgcaagctcg cgcgggcggc ggcggggatt
    10141 gtggcgcctg gaggattcct gatgctcgcg tcctgttcgc acaatatgtc tgcggaacgc
    10201 tttgccgccg aatgtgccgc gggcctcgcc cgttcgggac ggcgggcggc gctcatccgc
    10261 gaagccggtg ctggacccga tcatccggtg catcccatgc tgccggaaac ggcttatctc
    10321 aaaacgctga catatagcgt gggctagatt tcggtctgcg aacccagttc caccacgcgc
    10381 ccggggggca ggcgatagaa ctgcgccgct tccaatgccg atgcgtagag cttgatgaaa
    10441 agcttcgtct gccagcgtcg catcgacgag gcgcgcgccg gaaccagcgt ctcgcggcgg
    10501 atgaagaatg tcgttgcgtc gagatcgagc gaaagcccat aggcgcgcga accctccagc
    10561 gcggcgggca catcgggcgt ctggaagaaa ccataatgaa cctccacggt atagaagccc
    10621 ttgccgagct tcttgaccgt cagacgcttt tccggcggca cgaagggcac gtcctgcaca
    10681 tcgacacgca ggaaaatcac gcgctcatgc agcacctgat tgtgcttgag attgtgcagc
    10741 agcgagccag gcacttcatc gatcctgggc gtaacgaaga tcgcggtacc ggcgacccgg
    10801 atcggggact tgtcggcgcg ttcgagaaag cggtcggtgg agatgccgcc gccataggct
    10861 ttctccaaga gaacgcgccg tcccgcgcgc cagacttcca tgatgacgaa caccccggcc
    10921 gcgatggcga gcggcagcca ggcgccttcg accaatttca gcgacaccgc gccgaggaag
    10981 gtgagatcgg tcagaatgaa gacaccgaac gcggtcgcga tccacgccgg tttccaatgc
    11041 cattgccgga tgacgacaat caggccgaga aacgtcgtga tcgacatcat gcccgtgacg
    11101 gccatgccat aggccgcggc gagggcgtcc gaattcttat agatcagtac gatggcgacc
    11161 acaccgatca gcatcaatgt gttcgcccgc ggcacataaa tctggccgta atcggttgcc
    11221 gaggtgtgcc ggatctccat gcgcggcaat tggccgagct ggacggattg gcgcgtgatg
    11281 gaaaagacgc ccgaaatcac cgcttggctc gcgataacgg tggcgacggt cgcgagcgcc
    11341 accagcggat aatgaaagcc cggcggcgcg agcgcataga acgggctatc cagcgccgaa
    11401 ggatcgcgca gcaggaccgc cccctggccg agataattga ggatcagccc gggcatggtg
    11461 accgcgagcc agccgagccg gatcgccgtg cggccgaaat ggcccatgtc ggcgtagagc
    11521 gtttccacac ccgtgacggt caggaccacg gatcccagcg cgacgaacgc ggtccatggt
    11581 tccatcacga agagcctgat tccatcccag gggttcagcg cgaacaggat ttccggggtc
    11641 atcagtatgg agcgcaggcc gagaaggccc agcaccacga accagacaag gacgatgggt
    11701 ccgaacagcc ggccgagctt gcccgtgccg cgattttgga tgaagaacag cccgagcaga
    11761 atcaccagcg tgatgggaag caccagtggc ccaaaacgcg ggtcttcaat cttcagacct
    11821 tcgacagcgg aaagcaccga tatcgcgggc gtcagcatgc cgtcgccgaa gaacagagca
    11881 agcccgagca cgccggcaaa gccgatcagg gctttcatgc gccgcgacag gccggccgag
    11941 cgatgcgcaa gcgaagccag cgcaatcacg ccgccttcgc ccttgttgtc agctcgcagg
    12001 atgaagacga tatatttgat cgtgacgatg atgatgagcg accagaagat cagggagatc
    12061 gcgcccagca cggcgctaga catgggcatc tgcccgccgg tcgccagaac cgattggcgt
    12121 accgcataaa gcgggctggt gccgatatcg ccatagacga caccgagcgc gcccaatgtc
    12181 agccatgctt ggcgcttgcg ccaggcgagc cccgatactc tggacgctcc gccatggccg
    12241 cgggccgcct cggttgtctg catcaagctc acaggaaaca gtgctcgcgg atcattcgaa
    12301 cccgttgtcg gccgtaaggg ccttccttag gaaggatggt ccttacaacg cctcgccttt
    12361 cgggatagtt gccaacccga aggcggcgga aaatacgctt cgccctcaac tccgacaagg
    12421 cctaagctgc cgcagcagaa aaatctccta agtaactgaa ttacaaagga aaaatttcgt
    12481 ccttttaatc tagtgtgcga cgatagcgca aaagcgcgat cgccccgacc ccgccaagga
    12541 agattaacag cggccagata ttgggccaga tttccgtcca gccattgccc ttcagcagga
    12601 tgccgcgaac gatctgcagg aaatgggtat tgggaagggc ttccccaatc cattgcgccc
    12661 agtccggcat gccgcggaac gggaacatga atcccgagag caggatggat ggcagcagaa
    12721 aaaacaccgt catctgcatg gcctgaagct gatttcgcgc gacggtggaa aaggtgaagc
    12781 cgagcgccag attggccgcg atgaaaatcc ccgtggcgcc gacgagaagc aaaagcgagc
    12841 ctatcatggg cacatggaag atgaagcgtg cgcccaacag gatgagcgtt gcctggatgt
    12901 agcccaccac cacatagggg atgatcttgc ccagcatgac ctcaaagggg tgcgccggca
    12961 tggcgaggag gttctccagc gtgccccgtt cgcgctcgcg cgtcatggcc atggctgtca
    13021 ccatcaccat tgtcatggtc aggatgactg cgatcagtcc cggtacgata ttgtactggg
    13081 tgatcccctc ggggttgtag cgccgctgga tcacagcagt gaacggaagt accgcggcgc
    13141 ggatcgccag gggcccgacc aagtcatcct tcaacgccga agtcgcgagc tgcgtcatcg
    13201 cggcaagcgc atttcccgcc gccaccggat cggtcgcatc cgcctggatc agcacttcag
    13261 gattgaggcc gcggatcatg tcgcgcgcga aattcaccgg aatggtgatg acgaaggtga
    13321 cgtcgccccg atccagcatc cggtcgcctt cctcgggcga gcgcgccact gcgacgatat
    13381 cgaaatattc ggagttcttg agagccgccg cgatggagcg cgcatagcgg ctgtcgtcgg
    13441 cgacatggat ggcggcgggt agatgccttg ggttcatgtt gatggcaaag ccgaatgtga
    13501 ggagctgcaa tatcggcacc ccgaccatga tggcgaatgt catgtggtcg cggcgcatct
    13561 ggatgaattc cttcaccagg atggcgtaga ggcgggcgaa ggagaacatt ctcatgatgg
    13621 gttcttccct tgcgcatgat cgcggcactt cgccgcgatc attgcgcccg aatatgcgta
    13681 ccttgcagat ggatgaacac gtcttcgagg ctgggcgttt cctcatgcat ttcaacgccg
    13741 gactgtccgc gcaacgccgc gacagtctgt tcgagaagag tgcggtcgtg gccgcttgcg
    13801 tgcagcgcgg caccgaagaa tgccacatgc tcgacaccgg gcttgccctg gagctgcggc
    13861 agcaggcgcc tgacatcgcg cccctcgatg acgaacgtca cgaggcccga ttgcttgatg
    13921 acatcgtcga ccgtgccgcg tgcgatgata cggccctgat tgatataaac gatgcggtcg
    13981 cagcgctcgg cttcgtccat gtaatgggtg ctgacaagga ccgtgaggcc gctctgactg
    14041 agggcatgga tctgctccca gaattcccgt cgtgcctgcg gatcgacgcc agccgtgggc
    14101 tcgtcgagc
//
LOCUS       DASYEG010000107         4370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4383036, whole genome shotgun sequence.
ACCESSION   DASYEG010000107 DASYEG010000000
VERSION     DASYEG010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4383036"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VGM72_04080"
     CDS             <1..426
                     /locus_tag="VGM72_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264472.1"
                     /translation="QLELPDQNPNAVAFGIAPQLATLEALVNPSVDELLSTAAQSANG
                     TLEIIPPEAPLVIFVFSKNRVVPVRVTDFSITEEAFDIALNPIRAKVNLGLRVMSTDD
                     LGFQHKGGTIFLGYLRAREALAAKSGTANLSTLGITNLA"
     gene            440..790
                     /locus_tag="VGM72_04085"
     CDS             440..790
                     /locus_tag="VGM72_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264473.1"
                     /translation="MTTDPVQLLIEAGAIPSSPFAANSRYYSVVLGRCQRDANDAGIT
                     YVLRRFIPQQRDIPIAGTHIVHGGERPDLLAAQTLGDAELYWRVADANAVTDPFELTD
                     TPGARIAIPTPPGT"
     gene            805..1929
                     /locus_tag="VGM72_04090"
     CDS             805..1929
                     /locus_tag="VGM72_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264474.1"
                     /translation="MNGGVQLSLQIGPVPIPAPREVVDALVHVKVEDGSGGTQSGFEL
                     GFELPIRSPLRTLFLLTGGGSLPLMRVVLIVTINGAARSIIDGVTTNVETQPGEGGVA
                     KLIVKGKDLSALMDLIEIPGLPFPAMPPSARVLLILAKYAAFGVIPMVIPSILDIPPI
                     PVQQIPQQRGSDYAYVTKLAKDAGYVFYLEPGPAPGTSKAYWGPEIRVGEPQPALTTN
                     MDALSNVDQISFNFDKEKKTIPIVFFQEPVSKIPIGIPIPDVTPLNPPLGLVPPLPPK
                     IVKLDSTAHLSAPDALMAGLAYAGQHSDSVFGKGTLDVAKYGRLLKSRQLVGVRGAGL
                     PFDGLYYVKSVTHDIQRGSYKQSFSLARNALVSTFPTVPT"
     gene            1926..2438
                     /locus_tag="VGM72_04095"
     CDS             1926..2438
                     /locus_tag="VGM72_04095"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEY4264475.1"
                     /translation="MSTEPLYGKYRGTVTNNIDPLQIGRIQVMVADVAGFVPSTWAMP
                     CLMVAGINTGVFTVPMIGAGVWVEFERGDPDYPIWVGGYWGSAAEVPLLAHAVPPGIN
                     GITLQTPLKNGLVISDLPGPTGGILITTATNAMISVSDTGIIISNGKGASITLTGPTV
                     DVNLGALTVI"
     gene            2449..2766
                     /locus_tag="VGM72_04100"
     CDS             2449..2766
                     /locus_tag="VGM72_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264476.1"
                     /translation="MPAPILHLGATVLCSHAGQATPLTPFPRVTLSGQPAVTLASPYA
                     IAGCALTGTPNPPCVTGQWVAGATRVLAGGTPVVTMMGQSVCMAPGTPMLPVAAQTRV
                     LAT"
     gene            2777..3145
                     /locus_tag="VGM72_04105"
     CDS             2777..3145
                     /locus_tag="VGM72_04105"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEY4264477.1"
                     /translation="MSELDFPYHFDGLGRTATTSPEDHVRDLIEQVLFTAPGERVMRP
                     DFGSGLLALVFQPNSVTLAATTQMLIQGALQQYLSNLIAVQSVDVVNDDSTLRVTIVY
                     MLLSDRTTQTATFVAPGGAA"
     gene            3142..>4370
                     /locus_tag="VGM72_04110"
     CDS             3142..>4370
                     /locus_tag="VGM72_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264478.1"
                     /translation="MKYHCCDLRRLEVLKLSGSANAIEFLEVLDLASPPGAPRQQTLF
                     IRLLRPGFALTPDNLRISGGQRIRAVDIVWCAPANALPPEAEPGLVASVDDLARTLVV
                     RTESAGDFSIYIFAIVANSGSDDPPASFDPKLSSIEFSFKVECPSDFDCAQNPACPPE
                     PIVRPEIDYLAKDYQGFRRLMLDRLSLLTPSWTERSPADLGVALVELLAYAADNLSYR
                     QDAIANEAYLATARQRVSVRRHARLVDYFLHEGCNARAFVHFDVVGQNVALPSGTQLL
                     TRISNLGTVIAPGSQALRDAINSGAQIFETQHDAILDERLNQLFFYSWGDGGCCLPRG
                     TTSATLAGHADSLKPGDILVFQEMISPTTFKPEDADGSHRWAVRLTAVTLSRDPSGQL
                     FNEPPVDGPVDVTEIAW"
BASE COUNT          775 a         1422 c         1329 g          844 t
ORIGIN      
        1 caattggaac ttcccgacca gaaccccaac gcggtggcct tcggcatcgc accgcagctc
       61 gcgacgctcg aagccctggt caaccccagc gtcgatgagc tcttgtccac cgccgcgcag
      121 agcgcgaacg gcacactcga aatcataccg cccgaagcgc cgctggtgat cttcgtcttc
      181 agcaagaatc gcgtcgtgcc ggtgcgagtg acggatttct ccatcacgga agaggcgttc
      241 gacatcgcgc tgaaccccat ccgcgccaag gtcaatctcg gcttgcgcgt gatgagcacc
      301 gacgatctcg gcttccagca caagggcggc acgatcttcc tcggctatct gcgcgcgcgt
      361 gaggcactgg ccgcgaaatc cggcaccgcg aatttgagca cgctcggcat taccaaccta
      421 gcctgaaccg gataaagcga tgacaaccga tcccgtccaa ttgctgatcg aagccggcgc
      481 cattccgtcg agcccgttcg ccgcgaacag ccggtattac agcgtcgtgc ttgggcgctg
      541 tcagcgcgat gccaacgatg cgggcatcac ctatgtgctg cgccgcttca tcccgcagca
      601 gcgcgatatt ccaatcgcgg gcacgcacat cgtgcatggc ggggagcgtc cggatctgct
      661 ggctgcccag acgctcggcg atgcggagct ttactggcgc gttgccgatg ccaatgccgt
      721 gaccgatccg ttcgagctca ccgatacgcc gggcgcgcgt atcgccattc ccaccccacc
      781 cggaacctag gcgagcgata cattatgaac ggcggcgttc agctctctct gcaaatcggc
      841 ccggtgccca tccccgcgcc ccgagaggtc gtggacgctc tggtgcatgt caaagtggag
      901 gatggttcgg gcggcacgca aagcggcttc gagctcggct tcgagctgcc gattcgctcg
      961 cccttgcgca ccctgttcct gctgaccggc ggcggcagct tgccgctgat gcgcgtggtg
     1021 ctcatcgtca cgatcaatgg cgcggcgcgc tccatcatcg acggcgtgac caccaatgtg
     1081 gagacccagc cgggcgaagg cggcgtcgcc aagctgatcg tgaagggcaa ggacctttcc
     1141 gcgctgatgg atctcatcga gattccggga ctgcccttcc cggccatgcc gccttcggcg
     1201 cgcgtattgc tgatcctggc gaaatatgcc gcgttcggcg tgatccccat ggtgatcccg
     1261 agcattctcg atattccacc gattcccgtg cagcagattc cgcagcaacg cggcagcgat
     1321 tatgcctatg tgacgaagct cgcgaaggat gcgggctatg tcttctatct ggagccggga
     1381 ccggcaccgg gcacgtccaa ggcgtattgg gggcctgaaa tccgtgtcgg cgaaccgcag
     1441 cccgcgctga ccaccaacat ggatgcactc agcaatgtcg atcagatttc gttcaatttc
     1501 gacaaggaaa aaaagaccat ccccatcgtc ttctttcagg agccggtgag caaaattcca
     1561 atcggcattc ctattcccga tgtcacgccg ctcaatccgc cgctcgggct ggtgccgccg
     1621 ctcccgccga agatcgtgaa gctcgacagt accgcgcatt tgtccgcgcc cgacgcgttg
     1681 atggcggggc tcgcttatgc cggacagcac agcgattcgg tgttcggcaa gggcacgctc
     1741 gatgtcgcga aatatggccg gctgctgaag tcgcgccagc ttgtgggcgt gcgcggcgcg
     1801 gggctcccct tcgacggact ttattacgtc aagtccgtca cccatgacat tcagcgcggt
     1861 tcctacaagc agagcttctc gctcgcgcgc aacgccttgg tctcgacatt tccaacggtg
     1921 ccgacatgag cacagaaccg ctctacggca aatatcgcgg cacggtgacg aacaatatcg
     1981 atccgctgca gatcgggcgc atccaggtga tggtggccga tgtcgccggc ttcgtgcctt
     2041 cgacctgggc gatgccgtgt ctgatggtgg cgggcatcaa taccggcgtg ttcaccgtgc
     2101 cgatgatcgg tgcgggcgta tgggtcgaat tcgagcgcgg cgatcccgat tatccgatct
     2161 gggtcggagg ctattggggc agtgccgcgg aagtgccgct tctggcccat gcggtgccgc
     2221 cgggaatcaa cgggatcacg ctgcagacgc cgctcaagaa cggtcttgtc atcagcgacc
     2281 tgcccggacc gaccggcggc attttgatca ccacagccac caacgccatg atctcggtca
     2341 gcgacacggg aatcatcatc tccaacggca agggcgcgag catcacgctg accgggccga
     2401 ctgtggatgt gaatctcggc gcgctgacgg tgatctagga gagacgcgat gccggcaccc
     2461 attctccacc tcggcgcaac ggtcctctgc tcccatgcgg gccaggcgac ccctttaacg
     2521 ccgttcccgc gcgtgacgct ttccggccag ccggcggtca cgctggcaag tccttatgcg
     2581 attgcggggt gcgcactgac cggcacgccg aacccgccct gcgtcactgg acagtgggtg
     2641 gcgggcgcca cgcgcgtctt ggcgggcggc acgccggtcg tgaccatgat gggacagtcg
     2701 gtgtgcatgg cacccggcac gccgatgctt cccgttgccg cgcagacgcg cgtgctcgcg
     2761 acgtgatgag gcggcaatga gcgagctcga ttttccttat catttcgacg gcctgggccg
     2821 gacggcgacc acatcacctg aggatcatgt tcgggacctc atcgagcagg tgcttttcac
     2881 cgcgcccggc gaacgggtga tgcgccccga tttcggcagc ggtcttctgg cgctcgtgtt
     2941 tcagccgaac agcgtgacat tggcggcgac gacgcaaatg ctgatccagg gcgcgctaca
     3001 gcaatatctt tcgaacctga ttgcggtgca gtcggtcgat gtcgtcaatg atgattccac
     3061 cttgcgcgtg actatcgtct acatgctgct cagtgaccgc acgacccaga ccgcgacctt
     3121 cgtggcgccg ggaggggccg catgaaatat cattgctgcg atcttcgccg gctcgaagtg
     3181 ctgaagctga gcggctctgc caatgccatc gagttcctgg aggtgctcga tctcgcctcg
     3241 ccgccgggcg cgccgcggca gcaaacgctt ttcatccggc tgctgcggcc cggtttcgcg
     3301 ctcacgccgg acaatcttcg cataagcggc ggccagagaa tccgcgccgt ggacattgtg
     3361 tggtgcgcac cggccaatgc cctgccgccg gaggccgagc ccggcctcgt ggcgagcgtg
     3421 gacgatctcg cgcgcacgct cgtggtgcgc acagagagcg ccggagattt ctcgatctac
     3481 atttttgcca ttgtcgccaa ttccggcagt gacgatccac cggcgagttt cgatccgaag
     3541 ctgtcatcca tcgaattctc gttcaaagtg gaatgcccgt cggatttcga ctgcgcgcaa
     3601 aatcccgcct gcccgcccga gccgattgtc cgccccgaga tcgactacct cgcgaaggat
     3661 tatcagggct tccggcggtt gatgctcgat cgcttgagtc tgctgacacc gagctggacg
     3721 gaacgctcgc ccgccgatct gggcgtggcc ctggtcgagt tgctcgccta cgcggccgac
     3781 aatctctctt atcgccagga tgcgatcgcc aacgaagcct atcttgcgac ggcgcgtcag
     3841 cgcgtctccg tgcgccgcca tgcgcgcttg gtggattatt tcctccacga aggctgcaac
     3901 gcgcgcgcct tcgtgcattt cgatgtggtt gggcagaatg tggcccttcc cagcggcacg
     3961 caattgctga cccgaatttc caatctcggt acggtgatcg ctccgggcag tcaggcgttg
     4021 cgcgatgcca tcaatagcgg cgcccagatt tttgaaaccc agcacgacgc gattctggac
     4081 gaacggctga accagctatt tttctatagc tggggtgacg gcggatgctg tctgccgcgc
     4141 ggcacgacca gcgccacgct tgccggccac gccgattcgt tgaagcccgg cgacatcctg
     4201 gtgttccagg agatgatcag tcccacgaca ttcaaacccg aagatgccga cggctcgcat
     4261 cgctgggcgg tacggctcac cgccgtcacg ctttccaggg acccttcggg gcagctcttc
     4321 aatgaaccgc cggtcgatgg gcccgtcgat gtcaccgaaa tcgcctggga
//
LOCUS       DASYEG010000108        20890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2497026, whole genome shotgun sequence.
ACCESSION   DASYEG010000108 DASYEG010000000
VERSION     DASYEG010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 20890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20890
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2497026"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..373
                     /gene="rnpB"
                     /locus_tag="VGM72_04115"
     ncRNA           4..373
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGM72_04115"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            638..1120
                     /locus_tag="VGM72_04120"
     CDS             638..1120
                     /locus_tag="VGM72_04120"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264479.1"
                     /translation="MGLKGRKHQTVQPFVSTIVNKLDAKGRVSIPAPFRQILAQQTLR
                     GFYCFPSFVSPALEAFGEDLLADFQRRLGAVDPLFSEDYDAQAQAVVGETQLLNFDDE
                     GRVRLPDDFLAHAGIKDRVLFVGMIQKFQIWDPDRFEPVRLERIARARATRLGTGDAA
                     "
     gene            1117..2079
                     /gene="rsmH"
                     /locus_tag="VGM72_04125"
     CDS             1117..2079
                     /gene="rsmH"
                     /locus_tag="VGM72_04125"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEY4264480.1"
                     /translation="MSGAHVPVMLDEVMAVLQPRDDAIYVDGTFGGGGYSAALLASAR
                     CRVFGIDRDAEAIARGRVLADRSDGRLTLIHGRFSEMDELLEAQGVAKADGVALDLGV
                     SSFQLDQSARGFSFSSDGPLSMRMDDTNGKTAADLVNTLSEDALGDILRRYGEEKRAR
                     SIARAIVAARPIERTRELAGIAERVLGRGAHKIHPATRTFQALRIAVNDELGELERGL
                     AAAERVLKPQGCLAVVSFHSLEDRIVKRFLVERTGRTGRGSRHEPEQVRLHAPTFQTI
                     GKNPQVPGNEETRANPRARSARLRAAERNSAPAWNFSPTHGEPV"
     gene            2076..2504
                     /locus_tag="VGM72_04130"
     CDS             2076..2504
                     /locus_tag="VGM72_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264481.1"
                     /translation="MIRTLNFAFIVITSLVCLAVYRTAEQARVDQRALKTTQIAIVRE
                     TDALTVLGAEWARITQPARIQALTQRHLDLSDLPTVELSSLSQLPEKNPPLAAEGEIR
                     NAKAVVPQVPAVSQIIPASEPRIQPARLPDASFASFRTGT"
     gene            2508..4106
                     /locus_tag="VGM72_04135"
     CDS             2508..4106
                     /locus_tag="VGM72_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY4264482.1"
                     /translation="MAKRPAVPSLVRNRIAFAALFSAACFGIIAARLIDVMVFGATST
                     GTVVTLAHPMRADLVDRNGVLIARDLPVSDLYATPSAFWDTNEAAEELAAATGAEKGH
                     LKQIFAAKRGYILVQRGLTPDRRDAVMHLGLPGLVFEEGYKRYYPSGRIVAHAVGQVD
                     TDDHGVSGLELGLETRLRGARDAAPVQLSLDMRIQYVLEHEIEEAAHSFKTKAAGGIV
                     LNVRTGEVLALASMPDYEPNMRKLQAGDSIRNRMTQDVYELGSIFKIFAFTEAIEEQT
                     IRLDEPFEVGKPFKLGHYYIHDFERLGPSLPAAMVFADSSNIGTAQIEMRSGPAKQKP
                     FLKKLGLLDPLKTELPEVASPLYPRHWEVVEAATIGYGHGISVNPLGFVAAAAAVVNG
                     GTTIRPTFLKNDTVQHGERMISEETSRTMRQLLRLVVTDGTGKLADVPGYDVGGKTGT
                     AEKAENGSYAHHLLISSFCGVFPIGDPQYLVFIMLDEPHGNKQSGGFATGGATAAPAV
                     GHTIAKIAPLLGLHRTDTFAAANP"
     gene            4151..5608
                     /locus_tag="VGM72_04140"
     CDS             4151..5608
                     /locus_tag="VGM72_04140"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEY4264483.1"
                     /translation="MGKAEIVKSGVQEFRGLAADSREVKPGFLFAALSGIRTDGAHFI
                     EDAVTRGAVAVLGTPEIATIAKNLGVRFIGAANPRQRLAEIAAEFYGAQPAVIAAVTG
                     TNGKTSVASFLRQIWAFQGRRAASLGTIGVDSPSGHVSLGHTTPDPVRLHAELRRLKE
                     EGVDHLALEASSHGLDQYRLDGVKISAAAFTNITRDHLDYHASFAGYLAAKLRLFSEL
                     VREGGAAVVNADADHAQEFIAAAQSRGLRLLTVGRHGEDLRLIAQVPHVRGQDVTVRH
                     PGGEQKISLPLAGSFQASNALVAAGLALALGDLAQDVFAALTTLKGAPGRLELVARTS
                     QDAAIYVDYAHTPDAIETVLKALRPHVQGRLHIVFGCGGDRDRGKRSLMAEAAARLAD
                     EVVVTDDNPRSEDPAAIRREVLGGAPGAREMADRAEAIQYAVSSLREHDALVIAGKGH
                     EDYQIVGTEVRVFSDRDEAVKAARALGGMAVSEAA"
     gene            5605..6996
                     /locus_tag="VGM72_04145"
     CDS             5605..6996
                     /locus_tag="VGM72_04145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamyl-2,
                     6-diaminopimelate--D-alanyl-D-alanine ligase"
                     /protein_id="HEY4264484.1"
                     /translation="MSETALLWTAEEAAAATGGKAPVNWSAFGVSIDTRTLQPGDLFL
                     ALKGTRDGHAFVGAALEKGATAAMVAARPERVTADAPLLLVGDTQIGLERLGRASRKR
                     VRAKIVAVTGSAGKTTTKEMLRLMLAPSGMVAASAASYNNHWGVPLSLARMARDTAYG
                     VFEIGMNHAGEIRALVDQVRPHVALITTIAPAHLEYFGSVEAIADAKAEIFESVQPGG
                     TAILPRDNPQFERLKNRAQAAGVARVLTFGRGVDADARLIATESYSDGQKLTADIAGK
                     RIVCAVGAVGAHIALNAVASLLAVRELDAGMDAAAGALIGYHALKGRGARLVIGGIEL
                     IDESYNANPASMVAALDGLGQTQTRGRRIAVLGDMLELGENSEDLHRALAGAITAAHA
                     DLVFLCGSHMAALWQAIPPRVRGAYTEASATLAPELTRQLRTGDVVLVKGSFGSRMSV
                     IIDALKAREAARA"
     gene            7007..8095
                     /gene="mraY"
                     /locus_tag="VGM72_04150"
     CDS             7007..8095
                     /gene="mraY"
                     /locus_tag="VGM72_04150"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005297383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY4264485.1"
                     /translation="MFYHFLVPLADEYRVLNVFRYLTFRTGGAMMTGLFVCFVIGPAF
                     IRWLKISQGKGQPIRTDGPQRHIIEKQGTPTMGGLLILVAMVVATLLWADLSNLYIWA
                     VLMVTLFFGLLGLADDYLKVTKRSADGVSGRMKLLFQFLVAMAAVLFLMHLQAAPLTG
                     AVAVPFFKGVLIQLGWAFVIFGSLVVVGASNAVNLTDGLDGLAIVPVMIAAGTFLLIA
                     YLVGNVRFADYLQINYVAGTGELTILLGALIGASLGFLWFNAPPAMIFMGDTGSLALG
                     GVLGATAVAVKHEIVLAIVGGLFVMEAVSVMVQVVSFKTTGKRVFRMAPIHHHFEHLG
                     WREPTIVIRFWIIAVILALIGLSTLKLR"
     gene            8103..9539
                     /gene="murD"
                     /locus_tag="VGM72_04155"
     CDS             8103..9539
                     /gene="murD"
                     /locus_tag="VGM72_04155"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY4264486.1"
                     /translation="MIEVRCFKERTVGVFGLARSGLSAIRALRAGGAKVFAWDDNETG
                     RSLAEAESAAIEPWDGWPWAELSALILSPGVPLTHPQPHAIVTRARETGVEIIGDVEL
                     FARTVRPDRNTKGMAPVIAVTGTNGKSTTTALIGHILQSCGFDAQIGGNIGMPALDLA
                     TPGAETVYVLEVSSYQIDLSPGLVSNVAILTNLSSDHIDRHGSMAGYAAVKQKLLAQT
                     APDGCVAVGVDDPQSGAIYTKLSAERGNKVVAVSIGKVLGRGIFAIDGKLFDAWDQPA
                     TAVGDLTMATRLPGAHNWQNAALAYAAVKRIVRDPRAIMAAIIRFPGLAHRIEEVGRI
                     GKVRFVNDSKATNADAAARALACYSDIFWIAGGKPKEGGIVGLAPYYARLRRTYLIGE
                     AAQSFGRALQGQVDAVQSGTLEQALGMAFEDARSSNAPEPVVLLSPACASFDQFRDFE
                     QRGDRFREIVKTMMMDSQNLPTHEDVAS"
     gene            9536..10654
                     /locus_tag="VGM72_04160"
     CDS             9536..10654
                     /locus_tag="VGM72_04160"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY4264487.1"
                     /translation="MSLSRADNSGFARWWWTVDRVSLIAMLALMSIGLVLAFAASPAA
                     TGHGNSVGNFSYALKQVVFAATAVCILIGASLLSFKQARMAAAAIFALALVGAGLALL
                     IGPNVNGAHRWLEFGGFNLEPSEFLKPAFAVLAAAFLADKLKRGFPGEAWTLGFLVPA
                     ILILLFQPDVGQTALLTTLCVSMLFFAGLAYYWIVVLAAGGAGLATAAYFLHPHVRER
                     IAQFMDAGGYQTGLALKAFEAGGIGGVGPGAGTVKYRLPDAHTDFIFAVAGEEFGVLL
                     CILIAGLFGVVTIRLLLRAARSREPFAQLAGAGLALVIGLQAFINMGVSLSILPAKGM
                     TLPLISYGGSSLFGVALTLGFALGLTRQRPALPEEAAR"
     gene            10651..11751
                     /gene="murG"
                     /locus_tag="VGM72_04165"
     CDS             10651..11751
                     /gene="murG"
                     /locus_tag="VGM72_04165"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEY4264488.1"
                     /translation="MNAPFVLAAGGTGGHLFPAQALASELMRRGRRVVVMTDRRGPNY
                     GNAFPGAEITTIPAATFAGRSMLGRMTAFGVIALGVAAALTKFLNLRPRAVVGFGGYP
                     SLPVMLAASLVRVPSALHEQNAVLGRVNRLLAPRVKRIAATFPFARFAPDHPERVVFT
                     GNPVRVEAAALRAASYVPPGPGELIKLLIFGGSQGARALSEIVPAALARLSGDLRARL
                     DITQQSRAEDMEMVKQAYRVAALNAEVAKFFTDLPGRMAASHLVIARSGASTLSELTV
                     IGRPSILIPYPYATDDHQAANAAVLEKAGAAWVIKQEALDADGLARQLTEILSNPRLL
                     AARAQAAGALGHADAASRLADLAEALAKGGTV"
     gene            11748..13190
                     /gene="murC"
                     /locus_tag="VGM72_04170"
     CDS             11748..13190
                     /gene="murC"
                     /locus_tag="VGM72_04170"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEY4264489.1"
                     /translation="MSRLLPIDSQMRVPLSIGAIHFIGIGGIGMSGIAEVMKNLGYRV
                     QGSDSTDSANVKRLRDSGIPIAIGQCVENLGDAQVVVYSSAVKTDNPEFAAARTAGLP
                     VVRRAEMLAELMRLKRCVAIAGTHGKTTTTTMVAALLDAGGLDPTVVNGGIINAYGTN
                     ARLGAGEWMVVEADESDGTFLKLPATVAIVTNIDPEHLDFYGDFDHVRAAFQGFVENL
                     PFYGFGVMCADHPEVQAIIARIRDRRVITYGFSPQADARAVNVSFSQGASHFDAVFHD
                     RRKGTETTLGDLILPMPGEHNVSNAIAAIAVARELGVEASAIRKALNGFGGVRRRFTR
                     VGEWNGAAIIDDYGHHPVEIAAVLKAARQAYSGPIVGIIQPHRYTRLRDLFDQFCTCL
                     NSADTAIVAPVYAAGEAPIDGISRDALVEGLRAHGHKHVLTIDGPEDLPSLVAPLARA
                     GGAIVCLGAGSITGWAAGLESALRQWEAKL"
     gene            13190..14101
                     /gene="murB"
                     /locus_tag="VGM72_04175"
     CDS             13190..14101
                     /gene="murB"
                     /locus_tag="VGM72_04175"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEY4264490.1"
                     /translation="MPAGLNALPKVRGTYAEHASLKDLVWFRAGGAAEVLFRPADVED
                     LCAFMAAKPDDLPVTVIGVGSNLLIRDGGIGGVVIRLPASFGRIERLSGTHIRAGAAA
                     LDAAVARAAADAGIAGLEFLRGIPGTIGGALSMNAGCYGSEIKDVLMQARAVDPRGAL
                     VTLSPEEMGFSYRHSKPRGLVYVDAVLRGQSGEPEAIRSRMETLVAQRESTQPVRSKT
                     GGSTFTNPPGHKAWQLIDAAGCRGLTRGAAQVSEKHCNFLINTGAAAASDLEALGEEV
                     RDRVRTKFSIALEWEIKRIGRAKGGQT"
     gene            14098..15036
                     /locus_tag="VGM72_04180"
     CDS             14098..15036
                     /locus_tag="VGM72_04180"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEY4264491.1"
                     /translation="MIPFKRIAVLLGGRSPEREVSLVSGRACAKALREEGFEAVEVDA
                     GGDLAERLRVTNPDAVFNALHGRWGEDGCVQGLLELMEIPYTHSGVLASALAMHKEHA
                     KSAFRAAGLPVADSVVVDRHCAADSHQMARPYVVKPLNQGSSVGVFIVREGDNGPPTA
                     LDSPDWNFGESVMIEDYVPGRELTVAVMGDKGNAVRALAVTEIVPNLAFYDYDAKYAP
                     GGSNHVIPAKIPEIVGQEAMRLAERAHTALGCRGVSRTDFRFDDTKGSNRLIVLEVNT
                     QPGMTPTSLVPEQAAYKGMNFRALVRWMLEDASCDR"
     gene            15171..15944
                     /locus_tag="VGM72_04185"
     CDS             15171..15944
                     /locus_tag="VGM72_04185"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEY4264492.1"
                     /translation="MVLRRPIFTLTLSLVVIGGGVGLFAGGHVAQGLLRVQAVIAAPF
                     ADAGFALHEVTISGEERTTPEAASAALALTKGDSIFTVRPSAARARLLMLPWVADAEV
                     RRHFPDKVTVRLIEKRPFALWKNGNELSVVERSGAVITRVQRNAPSHLPNIAGVGAPE
                     AAAPMIDALAKEKAVQARLVAMERIGERRWDLHLSGGVTVRLPEEGWERQLSELERLI
                     VEKGVLERDIEMIDLRYPDNYVFRLHNGDSRPVPRERHA"
     gene            15956..17245
                     /gene="ftsA"
                     /locus_tag="VGM72_04190"
     CDS             15956..17245
                     /gene="ftsA"
                     /locus_tag="VGM72_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY4264493.1"
                     /translation="MGDAFRTRANGKANGAATSRARSGLIAALDVGSSKIACIIARAE
                     QGGIQVLGSALHESQGIKSGAVTNLDLAERSIREAVGAAEQLADLRIQDVILAVQCGQ
                     PKSLIARAEGTTAGELVNDVHLRALLGDAKRRCREDGYETIQAAPTGYVVDTSRGVSD
                     PRGMFCERLGVSVHAVAVRTGPLQNLRLAVERCHLSIARQLYGPYASGLATLTPDETS
                     LGVTLIDIGGGCTSVAVFMEDVLVHVDSVPLGGANITADVAKMLSAPLAAAERIKTLY
                     GSALGELDAAGDLIEVPLMGEEGASANDRVRRSVLTRIIQARLEEIFGEVQARLKKSG
                     FDVAAGRRAVLTGGGAQLAGTRELAAQLLNKQVRIGRPQTFPGLPAATAGPAYATALG
                     LLISGATSPPESNDPNPPQAEETPRRGFLRWVSDLFG"
     gene            17276..18877
                     /gene="ftsZ"
                     /locus_tag="VGM72_04195"
     CDS             17276..18877
                     /gene="ftsZ"
                     /locus_tag="VGM72_04195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY4264494.1"
                     /translation="MTINFMAPDANELTPRITVLGVGGAGGNAVNNMINAKLEGVSFV
                     VANTDAQALTQSKADHKIQLGAHLTEGLGAGAKPEIGRGAAEETLPEILERLSGAHMV
                     FITAGMGGGTGTGAAPVIARAVREQGILTVGVVTKPFHFEGDKRMRIAERGIEELQNY
                     VDTLIVIPNQNLFRVATERTTFAQAFAMADDVLHSGVRGVTDLIIMPGLINRDFADIK
                     TVMSEMGKAMMGTGEMDGEGRALRAAEAAISNPLLDDVSMKGARAVLVNVTGGPDLML
                     FEVDEAVNRIRAEVDPDANILFGSALSEDMEGRVRVSVVATGIDAESCVRLGDNVEKL
                     KPRRKAVDIEASPIASSLARGVFEPVAEISAQQPVANLALGISSEVEAILSTEAPELE
                     AVEPLNLGSEGAPIVEERAAPPLVMREDVAHERGRRSMFSGLFGGRKRIERTEPVVEP
                     AFERPRTPAMTQAVAQSIGRVPTPQQPVAQAARDSQQPVATQSRGSETRQPAQGKAGD
                     LFEGVAEDDRFEIPAFLRRQAKTGS"
     gene            18877..19731
                     /gene="lpxC"
                     /locus_tag="VGM72_04200"
     CDS             18877..19731
                     /gene="lpxC"
                     /locus_tag="VGM72_04200"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEY4264495.1"
                     /translation="MGKRRTLAAEVFATGVTLHAGVPVHMRLAPALPSSGICFRRTDI
                     VGSEPIMALWSNVVEARLGTVLGANGINVGVVEHLLAALAGAEIDDCSVELDGPEPPV
                     LDGDALAFLNLVNQAGVRETDGRQRIITVLKEVAVVTGDSSARLLPSDTRCFYFEIDF
                     SNAAIGRQSFTFDFTRENFRRDIAPARTFGFLNEAEQLRAMGYGRGADLSNTLVIDGD
                     RLINPERQRFPDEFVRHKILDVIGDLKLAGAPLIAHFEGRRSSHALNNALLRALFADR
                     KNYEWKDL"
     gene            19899..20747
                     /locus_tag="VGM72_04205"
     CDS             19899..20747
                     /locus_tag="VGM72_04205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEY4264496.1"
                     /translation="MPLKPLFFLSGARYTAAVIALGACSFLMTGCASDQDMAAQYVER
                     PIEKIYGDAWTMIDRQNWLEAGRQFDEVERQHPYSVWARRAILMSAYCYYEGNRYTEA
                     IDASTRFIQLHPGNKDVPYAYYLKAISLYEQIGDVERDQGKTDEALTALQDLIQRFPD
                     TEYARDARLKVDLARDHLAGREMTIGRFYLHEGEYLAAINRFRTVIDKYQTTTHAPEA
                     LERLTEAYLSLGVVKEAQTSAAVLGYNYPGSAWYMDAYTLLTEENIAPAEDQESWISR
                     AYRAVF"
BASE COUNT         3920 a         6046 c         6599 g         4325 t
ORIGIN      
        1 gcgtcagatg gccggatggc cgctggcctg tcgcgagaca ggctggagga aagtccgggc
       61 tccacggaaa cacggtgccg gataatgtcc ggcggaggcg actccaggga tagcgccaca
      121 gaaatgatac cgccaaccgg cgaaagccgc ggcaaggttg aaatggtgcg gtaagagcgc
      181 accgcgggtt cggcaacgaa accggcaagg caagccccac cgggagcaag accaaatagg
      241 gacggcactt ggcgtgttct ccgcgccgcc gtccgggtag gtcgcgcgag gcgttcggca
      301 acgaacgtcc cagaggaatg gccatccctc gcgtaagcga ggacagaacc cggcttacag
      361 gccgtctgac gccaatattt cgggggaggg cagtaacaga acaaagaaag aacggaaaca
      421 ttaggattcc atcaacgaat attgtagatt ggcgctgttg ttgtttcgtt ctaagggaat
      481 ggtaggccgc gaataagacg agactaaata ttgtaaagcc aaacccatat atttcctgtg
      541 tttttcgttg acgtggggca aaatcccatg ctatcccatc taatcccatg cgtcgtcgcg
      601 tggcgggtcg gctcttttgg agggtcgttc gcgtcgcatg ggtctgaagg ggcgcaagca
      661 ccagaccgtg caaccatttg tctccaccat tgtgaacaaa ctcgacgcca agggtcgcgt
      721 cagtattccc gcgccattcc gccaaattct cgcccagcaa actcttcgcg gtttctattg
      781 ctttccttct tttgtttctc cggcgcttga ggcgttcggc gaggacttgc tcgccgattt
      841 ccagaggcgc ctcggcgccg tcgatcccct gttcagtgag gattacgacg cgcaagccca
      901 ggcggtcgtc ggcgaaacgc aactcctgaa cttcgacgat gaggggcgcg tccgtctgcc
      961 cgacgatttc ctcgcgcatg ccggaatcaa agaccgggtg ctcttcgtcg gcatgatcca
     1021 aaaattccag atttgggacc cggaccgctt cgagccggtc cgtctcgaac gcattgcgcg
     1081 tgcgcgcgcg acgcggcttg gcacgggaga cgcagcatga gcggcgccca tgtcccagtg
     1141 atgctcgatg aagtgatggc agtgctccag ccgcgcgacg atgcgatcta tgtcgatggg
     1201 acattcgggg gcggcggcta ttcggcagcg ctcctcgcat ccgccagatg ccgggtcttc
     1261 ggcatcgacc gcgatgccga agccattgcc cgtggccgcg tgctcgccga tcgatccgat
     1321 ggccgtctga cgctcatcca cggccgcttc tccgaaatgg atgagcttct ggaagcgcaa
     1381 ggtgtcgcaa aggcagacgg cgtcgcgctg gacctcggcg tgtcgtcctt ccaactcgac
     1441 caaagcgcgc gcggcttttc ctttagcagc gacggcccgc tcagcatgcg gatggacgat
     1501 acgaacggaa aaaccgcggc cgatctcgta aacacgctgt ccgaagatgc gctcggtgac
     1561 atcctgcgcc gttatggcga agaaaaacgc gccagatcga tcgcgcgcgc catcgttgcc
     1621 gcacggccga ttgaacggac gcgcgagctt gcgggcatcg ccgaacgcgt gcttggccgc
     1681 ggcgcgcaca aaattcatcc cgctacgcgc acatttcagg cactgcggat cgcggtgaac
     1741 gatgaactcg gtgagctgga acgcgggctg gccgccgccg aacgtgtcct gaagccgcag
     1801 ggttgcctgg cagtcgtgtc gtttcattcg ctcgaagacc gcatcgtcaa acgcttcctg
     1861 gtcgagcgta caggccgcac aggccgtggt tcacgccatg aaccggagca ggttcgcttg
     1921 catgcgccga ccttccagac aatcggcaaa aatccgcaag tcccgggcaa cgaggaaacg
     1981 cgcgccaatc ctcgtgcccg ctcggcacga ctgcgcgccg ccgaacgaaa ttccgcgcct
     2041 gcctggaatt tctcgcccac ccatggagag cctgtatgat ccgcacgcta aatttcgcgt
     2101 tcatcgtcat caccagtttg gtatgtcttg cggtctaccg caccgcggag caggcgcgag
     2161 tcgatcagcg ggcgcttaag acaacgcaaa tcgccatcgt tcgtgaaacc gatgcgctaa
     2221 cggtgttggg cgcggaatgg gcacgcatca cgcagccggc acgtattcag gcgctgacgc
     2281 aacgtcacct cgacctttcg gatctgccga cggtggaact ttcgtcgctt tcccaacttc
     2341 ctgaaaagaa tccgccgctc gctgctgagg gcgaaatccg caacgccaag gcggtcgttc
     2401 cgcaagttcc ggcggtgtca cagatcattc ctgcgagcga accgcgcatt cagcccgccc
     2461 ggttgcccga tgcgagtttc gcgtcgtttc gcaccggaac atgatctatg gcgaagcggc
     2521 cggcagttcc atcgcttgtc cggaatagga tcgcattcgc ggccctgttc tcggcggcct
     2581 gcttcggcat catcgcagcg cgtttgattg acgtgatggt gtttggtgcc acctccaccg
     2641 gcaccgtggt caccctggcg catcccatgc gagcagacct ggtcgaccgc aatggcgtgt
     2701 tgatcgcacg cgatttgccg gttagcgatc tttatgcgac gccatcggca ttctgggaca
     2761 cgaacgaggc tgctgaggag cttgctgcag cgacgggcgc ggagaaaggg catctgaagc
     2821 agatctttgc cgccaaacgc gggtacattc tggtgcagcg cggactcaca ccggaccgcc
     2881 gggatgcggt gatgcatctg ggcctgccgg ggcttgtttt cgaagaaggc tacaaacgtt
     2941 actatccaag tggacgtatc gtcgcgcatg ccgtaggcca ggttgatacc gacgatcacg
     3001 gcgtgtcggg tttggagttg ggattggaga cccgtctccg tggtgcgcga gacgcagcac
     3061 cggtccaact ctctctcgat atgcgcattc agtatgtact ggaacacgag attgaagagg
     3121 cggcccactc gttcaagacc aaggctgcgg gaggcatcgt tctcaacgta cggacaggcg
     3181 aggttctggc gcttgcctcg atgcccgatt acgagcccaa catgcgcaaa ttgcaggctg
     3241 gagattccat ccgtaatcgc atgacgcaag acgtttacga gctgggctcg atcttcaaga
     3301 ttttcgcttt taccgaagct atcgaagaac agacaatccg gctggacgag ccattcgaag
     3361 tgggcaagcc tttcaagctc gggcactact acatccatga tttcgagcgg ctggggccga
     3421 gcttgcccgc cgccatggtg ttcgccgatt cctccaatat cggcaccgcg cagatcgaaa
     3481 tgcgctcggg cccggcaaag cagaagccgt ttctgaagaa acttgggctg cttgaccctc
     3541 tgaaaaccga gcttccggaa gtcgcctcgc ctctttaccc tcgccattgg gaggtggtgg
     3601 aagcggccac aatcggttac ggccacggca tctcggtcaa tccgttgggt tttgtcgcgg
     3661 ccgccgctgc ggtcgtcaat ggcggcacca ccatccgtcc aacgttcctc aagaacgaca
     3721 cggttcagca tggcgagcgt atgatctccg aggaaaccag ccgcacgatg cgtcagcttt
     3781 tgcggctcgt ggtaaccgat ggcacgggca agctggccga tgtgccgggc tatgacgtcg
     3841 gcggcaagac cggcaccgcg gaaaaagcgg agaacggttc ctacgcgcat catttgctga
     3901 tttcgtcctt ttgcggcgtc ttccccatcg gcgatccgca atatctggtt ttcatcatgc
     3961 ttgacgagcc tcacggcaat aagcaaagcg gcggtttcgc gaccggcggc gctaccgcag
     4021 cgcctgccgt cggacatacg atcgccaaaa ttgctccgct tctgggactg caccgaaccg
     4081 acacctttgc cgcggcaaat ccctgagatt ttcacaatgg aaccataatc aaggacgagt
     4141 gttgggtgcg gtggggaagg cggaaattgt gaagagtggc gtgcaggaat tccgcggact
     4201 tgcggcggac agccgcgaag tgaaacccgg attcttgttt gcggcccttt ccggcatccg
     4261 cacggacggc gctcacttca ttgaagacgc ggtcacgcgc ggggcggtgg cggttttagg
     4321 gacgccggag atcgcgacga ttgcgaaaaa cctcggcgtc cgtttcatcg gagccgctaa
     4381 tccccggcag cgtctggctg agatagcagc ggagttctat ggcgcgcagc ctgctgtgat
     4441 cgctgccgtt acgggcacca atggcaagac atcggttgcg tcgttcctgc gtcagatatg
     4501 ggcctttcag gggcgccgcg cggcaagcct cggcacgatc ggcgtcgatt ctccgtcggg
     4561 tcacgtgtcg cttggccata ccacgcccga tccggtgcgg ctccatgcag agcttcgccg
     4621 gcttaaggaa gagggtgtgg atcacctcgc gctcgaagcc tcgagccatg ggctagatca
     4681 gtaccggctc gacggcgtca agatttccgc agccgcgttc accaacatca cccgcgacca
     4741 tttagattat cacgccagct tcgccggcta cctcgcggcg aagttgcgac tcttcagcga
     4801 gctggtgcgg gaaggtggtg ctgcggtcgt gaatgcggat gccgaccatg cgcaagaatt
     4861 catagcggcg gcgcaatcgc ggggtctcag gctcctcaca gtgggcagac acggtgagga
     4921 tttgcggcta atcgcacaag tgccccatgt gagaggacag gatgtgactg ttcgccatcc
     4981 gggtggcgaa cagaaaattt ctctcccgct cgcgggttcg tttcaggcgt cgaacgcgtt
     5041 ggttgcggca ggactggctc tagcgttggg cgatctcgcc caagatgtgt tcgccgcgct
     5101 gacgacgctg aaaggcgctc ccgggcggct cgaactcgtg gcgcgcacga gtcaggacgc
     5161 ggcaatctat gtcgattatg cccacacccc ggatgcgatc gaaaccgttc tgaaggcact
     5221 ccggccccat gtgcaggggc ggctgcatat tgtctttggc tgtggcggtg atcgcgaccg
     5281 cggcaagcga tccctgatgg ctgaagccgc cgcgcgcttg gcagatgaag tcgtggtgac
     5341 ggacgacaat cccaggtccg aggatccggc tgcgatccgc cgcgaggtgc tcgggggcgc
     5401 gccgggtgcg cgggaaatgg ccgaccgcgc ggaggcgatt cagtacgcgg tgtcctccct
     5461 tcgcgagcac gacgcgctgg tgattgccgg caaagggcat gaggattatc aaatcgtcgg
     5521 taccgaagta cgtgtctttt ccgatcgcga tgaggcggtg aaggctgcgc gcgctttggg
     5581 tggcatggcc gtcagcgagg ccgcatgagc gaaaccgcgc tcttgtggac tgctgaggaa
     5641 gccgctgccg ccaccggcgg caaggcgccg gtcaattggt cggcttttgg cgtgtccatc
     5701 gatacgcgca cgcttcaacc cggtgatctt ttcctggccc tcaaaggcac gcgcgacgga
     5761 catgcgttcg ttggcgcggc gctggaaaaa ggagcgaccg ccgcaatggt ggcggcgcgg
     5821 cctgaacgcg ttacggcgga cgcaccgctg ctcctggtcg gcgatacgca gataggcctc
     5881 gaaaggctag gccgggcttc acgcaaacgc gtgcgcgcga agatcgtggc cgtcacaggc
     5941 agcgcgggga agacgacgac caaggagatg ctgcgcctga tgttggcgcc ctccggaatg
     6001 gttgcggcat ccgcagcctc ttacaataat cattggggcg tgccgcttag cctcgcacgg
     6061 atggctcgcg atactgctta cggcgtgttc gaaattggga tgaatcatgc cggggagatc
     6121 cgcgcactgg tggatcaggt ccggccgcac gtcgcgctga tcaccactat cgcgcctgct
     6181 catctggaat atttcggcag cgtggaagcg attgccgatg cgaaagcgga gatcttcgaa
     6241 tctgtccagc cgggtggaac cgcgatcctg ccccgtgata atccccaatt cgaacggctc
     6301 aaaaaccgtg cgcaggcggc gggcgttgcg cgcgttctca cgttcggcag gggtgtggat
     6361 gccgatgccc gcctgattgc gacggagtcg tatagtgacg ggcagaagct gactgcggac
     6421 attgccggaa aacgcatcgt ttgcgcggtc ggcgcagtgg gcgcgcatat tgcgttgaat
     6481 gcggtcgcct ctctgcttgc ggtacgcgaa ttggacgcgg gtatggacgc cgccgctggc
     6541 gcgctcattg gataccacgc actcaaagga cgtggtgcgc ggcttgtgat aggcggaatc
     6601 gaattaattg acgagagcta caacgccaat cccgcttcca tggtcgcagc tctcgatggg
     6661 ctgggccaga cgcagactag gggtcgccgt atagcggtgt tgggcgatat gcttgagttg
     6721 ggagagaact ctgaggattt gcatcgcgct ctcgcgggag ctatcaccgc cgcgcacgcg
     6781 gatctggtct ttctgtgcgg ctcacacatg gccgctctgt ggcaggcgat accgccacgt
     6841 gtacgcggtg cctacaccga agcttccgcg acactcgccc ccgagctcac gcgccaattg
     6901 cggacaggcg atgtggtgct ggtcaaaggc tcatttggca gccgcatgtc ggtgatcatc
     6961 gacgcgctca aagcgcgcga agccgctagg gcttaggggg gaacgcgtgt tctaccattt
     7021 tctggtgccg ctcgccgacg aataccgggt cctcaacgtt ttccggtatc tcacttttcg
     7081 tacaggcggc gcgatgatga ctggactctt tgtctgcttc gtcatcgggc cggccttcat
     7141 ccgatggttg aagatcagtc agggcaaagg ccagccgatt cggactgacg gtccgcagcg
     7201 ccacattatc gagaaacagg gcacgcccac catggggggc ctcctcatcc tcgtcgccat
     7261 ggtggtggcc acactgctgt gggcagacct gtccaatctt tacatttggg ctgtgctgat
     7321 ggtcacactc ttctttgggc tgctcgggct cgcggacgat tatctgaaag tgacgaagcg
     7381 ttcggccgat ggcgttagcg gccgcatgaa gctgctgttc cagttcttgg ttgccatggc
     7441 ggccgtgctt ttcctcatgc acttgcaggc ggctccgctc accggagcgg tcgcggttcc
     7501 tttcttcaag ggtgtgttga tccagcttgg ttgggcattt gtcattttcg gctccctcgt
     7561 agtggttggc gcatccaacg cggtgaatct tacggacggt ctcgacgggc ttgccatcgt
     7621 gccggtcatg atcgcggcag ggacgtttct gctcatcgca tatctcgtcg gcaacgtccg
     7681 tttcgccgac tatttgcaaa tcaactatgt tgctggcacc ggagaactca ccattcttct
     7741 cggtgctctg atcggggcaa gtctaggttt tctttggttc aacgccccgc cggcgatgat
     7801 cttcatgggt gatacgggat cgctcgcgct tggtggggtt ctgggtgcaa cggccgttgc
     7861 ggtgaagcac gaaatcgtat tggccattgt cggtggtttg ttcgtgatgg aagcggtctc
     7921 ggtgatggta caggtggttt cttttaagac caccggcaaa cgcgtttttc gcatggcccc
     7981 aattcatcat cacttcgagc atctgggctg gcgcgagccc accattgtga tccgtttctg
     8041 gattatcgcg gtgattcttg cattgatcgg tctttcgact ctgaaactgc gttgagatgg
     8101 tcatgatcga agttcgatgc ttcaaggaac gaacggtcgg cgtattcgga ctggcacgct
     8161 cgggcctctc cgcgatccgg gcactacgcg cgggcggcgc gaaagttttt gcctgggacg
     8221 acaacgaaac tggacgttcc ctcgcagaag ctgaaagcgc ggctatcgaa ccatgggacg
     8281 gctggccatg ggcagaattg agcgcgctta tcttgagtcc cggggtgccg ctgacgcatc
     8341 ctcaaccgca cgcaatcgtg acgcgcgcgc gcgaaacagg tgtcgagatt atcggcgatg
     8401 tggaattgtt cgcacggacg gtaaggccgg accgcaatac gaaggggatg gcgccggtca
     8461 tcgcggttac cggcactaat ggcaaatcta cgactacggc gctgatcggc cacatcctcc
     8521 agagctgcgg attcgatgcg caaatcggcg gcaatatcgg catgccggcg cttgatctcg
     8581 cgacgccggg cgcggaaacc gtctatgtgc tggaggtttc ctcttatcag atcgatctgt
     8641 cgccgggact tgtttcgaat gtagcgatcc tcaccaatct ttcgtcggat cacatcgacc
     8701 gccacggatc catggccggc tatgcagcgg tgaagcagaa gctgctggca cagaccgcac
     8761 cggatgggtg cgtcgcggtt ggagtggacg atccgcaatc gggagcgatt tacaccaagc
     8821 tttcggccga acgcggcaat aaagtggtgg cggtgtccat cggcaaggtg ctcggccgcg
     8881 gtattttcgc aatcgatggg aagctcttcg atgcgtggga ccagccggcg acggcagtcg
     8941 gcgatcttac gatggccacc cggcttcccg gggcgcacaa ttggcagaac gcggcgctcg
     9001 cttacgctgc agtcaaacgg atcgtgcgcg atccgcgcgc catcatggct gcgatcatcc
     9061 gttttccggg actcgctcac cgtatcgaag aagtagggcg aattggcaaa gtgcgctttg
     9121 tcaatgattc caaggcgacc aatgcagacg ctgcggcccg cgcgcttgca tgctactccg
     9181 atatcttctg gatcgccggc ggcaagccca aggaaggcgg catcgtgggc ctcgcgccct
     9241 attacgcaag actccgccgg acctatctga tcggagaagc ggcgcaaagt ttcgggcgcg
     9301 cactgcaggg gcaggtcgat gcggtgcaat caggcacact tgagcaggcg ctcggaatgg
     9361 cgttcgagga tgcacgatcg agcaatgccc ctgaaccggt ggtgcttctg tcgcccgcct
     9421 gcgcatcttt cgatcagttt cgcgatttcg aacagcgcgg cgacaggttc cgcgaaatcg
     9481 tcaagactat gatgatggat tcgcaaaatt tgccaacgca cgaggacgtt gcttcatgag
     9541 tctttcgcgc gccgataatt ccgggtttgc ccgctggtgg tggacggtcg accgcgtcag
     9601 cctgatcgcc atgttggcgc tgatgtcgat cggcctggta ctcgcatttg ccgccagccc
     9661 ggccgcaacg ggccacggca attcggtcgg aaatttctcc tatgcattga aacaggtcgt
     9721 ctttgccgca acggccgttt gcattctgat aggcgcctca ttgctcagtt tcaaacaggc
     9781 gcgtatggcc gcggcggcga tcttcgctct cgcgttggtc ggagctggac ttgcgcttct
     9841 tatcgggcca aatgtcaacg gggcccatcg ctggctcgaa tttggcggtt tcaacctcga
     9901 gccttcggag tttctcaagc ccgctttcgc tgttcttgcc gcggcttttc tcgcggacaa
     9961 gctcaaacgt ggtttcccgg gtgaagcctg gacgctgggg ttcttggtgc ctgccattct
    10021 gattttattg ttccagcccg atgtcgggca gacggctctg ctcacgacgc tctgcgtatc
    10081 catgctattt ttcgcgggtc ttgcctatta ttggatcgtg gttctggcgg ccggaggagc
    10141 tggtctcgcc accgcggcgt atttcttgca ccctcatgtg cgcgaacgta tcgcccaatt
    10201 catggatgcg ggcggctatc aaacaggtct cgctctaaag gctttcgagg caggcggtat
    10261 cggtggtgtc gggcccggcg cgggcacggt taaataccgg cttcccgacg cgcacaccga
    10321 tttcatcttc gcagtcgcgg gcgaggaatt cggtgtcctg ctttgcatct tgatcgcggg
    10381 tctcttcggg gtggtgacca tacgcttgtt gctgcgtgcc gcacgctcgc gcgagccttt
    10441 cgctcaactt gctggcgcgg gtctggccct tgtcattggg ctccaggcgt tcatcaatat
    10501 gggtgtctcc ctgtcgattc tgccagcgaa aggtatgacc ctgccgctga tctcctatgg
    10561 cggctcgtcg ctcttcggcg tggccctgac tttagggttt gcgcttggcc tcacccggca
    10621 acgtcccgct cttcccgaag aggctgcgcg atgaatgccc cgtttgtgct cgcggccggt
    10681 ggcaccggcg gtcacctttt tcccgcccaa gcgcttgcga gtgaattgat gcgccgtggc
    10741 cggcgggtgg tggtgatgac cgacaggcgt gggcccaatt acggcaacgc ctttcctggc
    10801 gccgaaatca caacgattcc tgcagcgaca tttgccgggc ggtccatgct gggccgtatg
    10861 accgcatttg gcgtaatcgc actgggggtt gcggcggctt tgacgaaatt tctcaatctg
    10921 cgaccccgcg cggtggtggg gtttggcggc taccccagcc tgccggtgat gctggcagcc
    10981 tcacttgtgc gggtgccttc ggcattgcat gagcagaatg ccgtgcttgg acgcgtcaac
    11041 cgcctgcttg cgccgcgcgt gaaacggatt gcggccacgt ttccgtttgc gcgtttcgct
    11101 ccggatcatc ccgaacgcgt cgtcttcacc ggcaatccgg tgcgggtcga agccgccgct
    11161 ctacgggccg cttcttacgt gccgccaggc ccgggggaac tcataaaact gcttattttt
    11221 ggcggcagtc agggcgcacg tgcgctgtcg gaaatcgtac ccgcggcgtt ggcacgcttg
    11281 tctggcgatt tacgggcccg actcgacatc acccagcaga gccgcgccga agatatggag
    11341 atggtgaaac aggcttatcg cgtggcggcg ttgaatgcgg aagtggcgaa gttcttcacc
    11401 gatttgcctg gacgcatggc ggcatcccac cttgtcatcg cgcgaagcgg cgcgtccacg
    11461 ctttcggaac tgacggtgat cggccgtcca tcgatcctca tcccctatcc gtacgcgaca
    11521 gatgatcatc aggcggccaa cgcggctgtg cttgaaaagg ctggcgcggc ctgggtcatc
    11581 aagcaggaag cattggacgc cgacgggctt gcgcggcaac tgaccgaaat cctgtcaaat
    11641 ccgcggctgc ttgcggcgcg tgctcaagcc gccggagcgc tcggccatgc ggacgcagct
    11701 tcgcgcctcg cggatcttgc tgaggccctg gcgaaaggcg gcaccgtatg agccggctat
    11761 tgccaatcga ttcacagatg cgggtgccgc tttccattgg cgcgatccat ttcatcggga
    11821 tcggtggcat cggtatgagc ggcatcgccg aagtgatgaa gaatctcggc taccgggtgc
    11881 agggttcgga ttccacagat agcgcaaatg tcaaacgcct gcgcgattca ggaatcccga
    11941 tcgccatcgg ccagtgcgtg gaaaatctcg gtgatgcgca agtcgtcgtc tattcctccg
    12001 cagtaaaaac cgacaatccc gaattcgcgg ccgctcgtac ggcggggctg ccggtcgttc
    12061 gccgtgccga gatgctggcc gaactcatgc ggctgaaacg ttgtgtcgcc atcgcgggca
    12121 cccacggcaa gaccacgaca accaccatgg tggcggcgtt gctcgatgcc ggcgggctcg
    12181 acccgactgt ggtgaatggc ggtatcatca acgcctacgg aaccaatgcg cgtcttggcg
    12241 caggtgagtg gatggtcgtg gaagccgacg aatctgacgg caccttcctg aaacttccag
    12301 cgacggttgc cattgtcaca aacatcgatc ccgaacacct cgatttctat ggcgatttcg
    12361 accatgtccg tgccgctttc cagggtttcg tcgagaacct gcccttctac ggctttggtg
    12421 tgatgtgtgc cgatcacccg gaggtacagg cgatcatcgc ccgtattcgc gaccggcgtg
    12481 tcatcaccta tggcttcagc cctcaagcag acgcacgcgc cgtgaacgtg tcgttttcgc
    12541 agggagcatc gcatttcgat gcagtctttc acgaccggcg caaaggcacc gagaccacgc
    12601 tcggcgatct tattttgccg atgcctggcg aacacaatgt ctccaatgcc atcgcggcga
    12661 tagcggtcgc gcgcgaactg ggcgtggaag ccagcgccat tcgtaaggcg ctgaacggct
    12721 tcggcggggt aaggcggcgg ttcacgcgcg tcggtgaatg gaacggcgca gccatcatcg
    12781 acgattatgg ccatcatccc gttgaaatcg ctgccgtcct gaaagcagcg cgtcaagctt
    12841 attcgggtcc aattgtcggg atcattcagc cccatcgtta tacgcggctg cgtgacctgt
    12901 tcgatcagtt ctgcacgtgc ctcaacagtg ccgacacggc gattgttgca cccgtctatg
    12961 cggccgggga agcgcccatt gatggcataa gccgcgatgc gttggttgaa ggtctgcggg
    13021 cgcacgggca taagcatgtc ctcactattg acggtcccga ggacctgccg tcgcttgtcg
    13081 cgccgctcgc cagggcaggc ggtgcgattg tctgcctcgg tgcaggctcc atcacaggct
    13141 gggccgctgg tctcgaatcc gcgctgcgcc agtgggaagc gaagctgtga tgcccgcggg
    13201 actcaatgcg ctgccgaagg tgcgcggcac ttatgccgaa cacgcgagcc tgaaagacct
    13261 tgtatggttc cgcgcgggcg gagccgccga ggttctcttt cgtccggccg atgtggaaga
    13321 tctttgcgcc ttcatggcgg ccaagcccga cgatctgccg gtgaccgtga tcggcgtcgg
    13381 ctccaacctc ttgatccgcg atggaggcat cggcggcgtc gtcattcgcc ttccggcttc
    13441 tttcggccgc atcgaaaggc tgagcggcac acacatccgc gctggggcgg ccgcgctcga
    13501 tgcggcggtc gcgcgtgcag cggctgatgc cggcatcgcc ggccttgaat ttctgcgcgg
    13561 tattccgggg accatcgggg gcgccctgag catgaatgcc ggctgctatg gctctgaaat
    13621 aaaggacgtg ttgatgcagg ctcgcgccgt ggatccgcgc ggagcattgg tcacgctcag
    13681 cccggaggag atgggcttca gctatcggca ttcgaaaccg agaggcttgg tttatgtcga
    13741 cgcggtcctg cgaggacaat ccggagagcc cgaagcgatc cgttctcgga tggagacgtt
    13801 ggtcgcacag cgcgagagca cccagccggt gcgcagcaag acggggggct ctacttttac
    13861 caatccgccg gggcacaagg cctggcagtt gatcgatgct gccggatgcc ggggcttgac
    13921 ccgtggcgcg gctcaagtct ctgagaagca ttgcaatttt cttatcaaca cgggcgccgc
    13981 cgccgccagc gatcttgaag cgttgggcga ggaagttcgc gaccgcgtga ggacgaaatt
    14041 cagcatcgcg ctcgaatggg aaatcaaacg catcgggcgc gcgaagggcg ggcaaacatg
    14101 atccccttca aacgtatcgc tgttctgctc ggggggcgat cgcccgaacg cgaagtgagc
    14161 ctcgtttcgg gccgcgcctg cgccaaagcg ctgcgtgagg aaggctttga ggccgtcgaa
    14221 gtcgacgccg gcggggacct tgccgaacgt ctgcgtgtga cgaatcccga cgccgtgttc
    14281 aacgccctgc atggccgctg gggggaagac ggctgcgtcc aggggctgct ggagctgatg
    14341 gaaatcccct acacccattc aggcgtactt gcgtccgccc tcgcgatgca caaggaacat
    14401 gccaaatccg cttttcgcgc tgcgggcttg ccggttgccg acagcgtggt ggtggatcgc
    14461 cattgcgccg ccgacagcca ccagatggcg cggccttatg tagtgaagcc gctcaatcaa
    14521 ggttccagtg tcggcgtttt catcgtgcgg gagggcgata atggtccacc caccgcactc
    14581 gattcgccgg attggaattt cggtgaatcc gtgatgatcg aggattatgt gccgggccgc
    14641 gaactgaccg tggccgtgat gggagacaag ggcaacgccg ttcgcgcatt ggcagtgacc
    14701 gagatcgttc cgaacttggc gttctacgat tacgacgcaa aatatgcgcc gggtggttca
    14761 aaccatgtga tccccgcgaa aattcctgaa atcgtcgggc aggaagctat gcgcttggct
    14821 gaacgtgcac ataccgcgct cggctgccgg ggggtttcgc gcaccgactt tcgttttgac
    14881 gacacaaaag gctcgaatcg gctcatcgtg ctcgaagtca acacgcagcc gggcatgact
    14941 ccgacgtctt tggtgccgga gcaggccgca tacaaaggca tgaacttccg ggccctcgtg
    15001 cgctggatgc tggaggacgc ttcgtgcgac aggtaaaggc gaaggccacg cgattggaac
    15061 ggcccccagg cagcagccgt cgcggcaagt ccgctatacg cccggttgat tctcgtgttt
    15121 cgcgtagggc caaatctgct gagaccttct ggggaggttt gatacagcgc ttggttcttc
    15181 gccggcctat tttcactttg acgctgagtc tcgtggtgat cggtggcgga gttggattgt
    15241 tcgccggcgg gcacgtcgcc caagggcttt tacgggtgca agccgtcatc gctgcgccct
    15301 ttgcggatgc cggttttgca ttgcacgagg tcacgatctc gggcgaggag cgtacaactc
    15361 cagaggccgc gtcggcggcg ttggcgctga ccaaaggcga ttctattttc actgtgaggc
    15421 cgagcgcggc gcgggcccgg ctcctgatgc ttccttgggt ggcggatgca gaagtacggc
    15481 gccattttcc ggacaaggtt acggttcgct tgatcgaaaa gcgtcccttc gctctctgga
    15541 agaacggaaa cgaactctcc gtggtggaac gctccggggc ggtgattacc cgggttcagc
    15601 gtaatgctcc ttcccacttg ccgaacattg ctggcgtcgg cgctcccgaa gccgcagctc
    15661 ccatgataga cgcactggcg aaagaaaagg cggttcaggc acggcttgtc gccatggagc
    15721 gtatcggtga gcggcgctgg gacctgcatc tttccggcgg tgtcaccgtg cgccttccgg
    15781 aagaagggtg ggagcggcaa ctttcggagc ttgagagact gatcgtagaa aaaggcgtcc
    15841 tcgaacgcga tatcgagatg atcgatcttc gctatcccga taattatgtg tttcgattgc
    15901 ataatggtga ttcgcggccc gtgccgcgag aacgtcatgc gtaggtagag gcccgatggg
    15961 ggacgcattc cgcacacgag cgaatggcaa ggcgaacggc gcagcaacga gtcgcgcacg
    16021 cagcggcctg atagctgccc tcgacgtggg ctcgtccaag atcgcctgta tcatcgcacg
    16081 cgctgaacag ggtggcatcc aggtgctggg ttcagccttg catgagtcgc agggcatcaa
    16141 atcaggtgcc gtgaccaatc ttgatctcgc ggagcgttcc atccgcgaag ctgttggcgc
    16201 ggccgagcaa ttggcggatt tgcgcattca ggacgtgatt ctcgcggtgc agtgtggaca
    16261 gccgaaaagt ctgatcgcgc gcgccgaagg cacaaccgct ggagaattgg tgaatgatgt
    16321 gcacttgcgt gcacttctgg gagacgcgaa gcgccgttgc cgggaagacg gctacgaaac
    16381 tatccaggcg gcgcccaccg gctatgtggt cgatacctcg cgaggtgtat ccgatcctcg
    16441 cggtatgttc tgcgaacggc tcggcgtctc agtgcatgcc gtcgcagtgc ggacgggacc
    16501 gttgcagaat ctgcgccttg cagtggagcg ctgtcatctc agtatcgccc gtcaattgta
    16561 tggaccctac gcaagtgggc tggcgacttt gaccccagac gagacatcgc taggcgtgac
    16621 cctgatcgat attggcggcg ggtgcacgtc ggtcgctgtt ttcatggaag atgtgctggt
    16681 gcatgtggat tcggttccac tcggtggagc gaatatcacc gccgacgtag caaagatgct
    16741 gtccgcgccc ctcgcagctg cggagcgcat caagacgctc tatggttccg cgctcggcga
    16801 actcgacgca gcaggtgatc ttatcgaagt gccgctgatg ggcgaggagg gcgcgtctgc
    16861 caacgaccgt gtacgtcgct cggtgttgac ccggatcatt caggcacggc tcgaagaaat
    16921 ctttggcgaa gtgcaggctc gcttaaagaa gagcggtttc gatgtcgccg cgggaaggcg
    16981 ggctgtgctg accgggggcg gcgcacagct tgccgggaca cgcgaactgg cagcacagct
    17041 tttgaacaag caggtccgca ttggacggcc acagaccttt ccgggacttc ccgcagcgac
    17101 tgccggtccc gcttatgcca cggctttggg gttgcttatt tcgggcgcaa caagccctcc
    17161 ggagagcaac gatcccaatc cgccgcaggc ggaggaaact ccgcggcgcg gcttcttacg
    17221 ctgggtaagt gacctttttg ggtgaatacg tgacgttaaa tctcggaggc atgacatgac
    17281 gatcaatttt atggcgccgg acgccaacga actgacaccg cgcataaccg tgcttggtgt
    17341 cggcggcgcc gggggcaatg ccgtcaacaa tatgatcaat gcgaaactcg aaggagtgag
    17401 tttcgtcgtt gccaatactg atgcgcaggc gctgacccag tccaaggcgg atcataagat
    17461 tcaattgggc gcgcatttga ccgaaggtct cggtgcgggt gcgaaaccgg agatcggccg
    17521 tggtgctgcg gaggagaccc tgccggaaat cctggaacgg ctgtcgggag cccacatggt
    17581 tttcatcacg gcgggtatgg gaggcggtac gggtacgggc gctgctccgg tgatcgcgcg
    17641 ggcggtgcgc gaacagggga tcctcacagt gggcgtggtg acgaagccat tccactttga
    17701 aggcgataag cgcatgcgga tcgccgaacg cggtatcgaa gaactgcaaa attatgtcga
    17761 cacgctgatc gtcatcccca accagaacct tttccgggtt gccaccgagc gcaccacttt
    17821 cgcgcaggcc tttgcgatgg ccgacgatgt gctgcattcg ggcgtccgcg gcgtcaccga
    17881 tttgatcatc atgccaggcc ttatcaatcg cgatttcgcc gatatcaaga cggtgatgtc
    17941 cgaaatgggc aaagctatga tgggtacggg cgaaatggac ggggagggcc gtgcccttcg
    18001 cgcggccgag gccgcgattt ccaacccgtt gctggacgat gtctccatga aaggcgcacg
    18061 ggcggtgctg gtgaacgtga ccggtgggcc cgatctgatg ctgttcgagg tcgatgaagc
    18121 ggtcaatcgc atccgtgccg aggttgatcc ggacgccaat atcctgtttg gaagtgcgct
    18181 ttcagaagat atggagggcc gggtccgcgt ttcggtggtt gccaccggca tcgatgcgga
    18241 atcctgtgtg cgcctgggcg ataatgtcga gaaactgaaa ccgcgccgta aagcagtgga
    18301 catcgaagcg tcgcctattg cgtcgtcgct cgcgcggggt gttttcgaac cggtcgcgga
    18361 aatctctgcc caacagcctg tcgccaatct tgcgctcgga atttcctctg aagtggaggc
    18421 catactgtcc acggaagctc ctgagttgga agccgtggag cctctaaatc tcggcagcga
    18481 aggggcgccg atcgttgagg agcgcgcggc tccgccgctg gtgatgcgcg aggacgtggc
    18541 gcatgagcgc ggaaggcgga gcatgttcag cggattgttt ggcgggcgca aacgcataga
    18601 acgtaccgag cctgtggtgg aaccagcgtt cgaacgtccc cggacgccgg caatgacgca
    18661 agccgtcgcg cagtcgatcg ggcgtgtgcc gacgccacag caaccggtcg cgcaagcggc
    18721 cagagattcc caacagccgg tggcgacaca atcgcgcggc agcgaaacac gtcagcccgc
    18781 ccaaggtaaa gccggtgatc tcttcgaggg cgttgccgag gatgaccggt ttgagattcc
    18841 ggcctttctg cgtcgccagg caaaaacagg ttcttgatgg ggaaacgccg gacgctcgct
    18901 gcagaggttt tcgccactgg cgtgaccctg catgcgggtg ttccggtgca tatgcggttg
    18961 gctcctgctc tgccgtcatc cggaatttgt ttccgccgga cggatattgt cggatcggaa
    19021 cccattatgg ctctgtggtc gaatgtcgtc gaggcgcggc tgggcaccgt cctgggcgcc
    19081 aacgggatta acgttggggt cgtggaacat ctgctcgctg cgttggcggg cgcggaaatc
    19141 gacgattgta gcgtcgagct agacggacct gaaccacccg ttcttgatgg cgatgccctg
    19201 gcgtttctga acctcgtgaa ccaggctggc gtacgagaaa cggacggtag gcaacgaatc
    19261 atcacggtgc tgaaagaggt cgcggttgtg actggagata gctcggcgcg gctgctgcca
    19321 tccgatacgc gctgtttcta ttttgagatt gatttctcca atgcggcaat cgggcggcaa
    19381 agcttcacat tcgatttcac gcgtgaaaat tttcgccgtg acatcgcgcc ggcgcgcact
    19441 ttcggcttct taaacgaagc tgaacaattg cgcgctatgg gttacgggcg tggtgccgat
    19501 ctttccaaca ccctcgtaat cgatggcgac aggctgataa atccggagcg gcaacggttc
    19561 cccgatgaat ttgtccgcca caagatcctg gatgtgatcg gcgatctgaa acttgcgggt
    19621 gctccgctaa tcgcgcattt cgagggacgg cgttctagcc acgcgctcaa caatgcgctt
    19681 ttgcgtgcgc tctttgcgga ccggaaaaac tatgaatgga aagacttgta gctatacacc
    19741 tacaggtaga gaatgatata aactctcaac cggtgcagac tgatatggtt taaaagtgaa
    19801 agcggcggcc cgcggcatga tctagaccgg ttgttcccgc cagtggcata aaccatttgc
    19861 attgaacgcg ccttactcaa cgcctctagg attcttccat gcccctgaag ccgctgttct
    19921 ttctgtccgg tgcccgatac accgccgcgg ttatcgcact tggtgcctgt agttttctga
    19981 tgacgggttg tgccagcgac caggacatgg ccgcccaata tgtcgagcgc ccgatcgaaa
    20041 aaatttatgg cgacgcttgg acgatgattg atcggcagaa ctggctcgag gcgggacgcc
    20101 agtttgacga ggtggaacgg caacacccct attcggtatg ggcgcgtcgc gccatcctga
    20161 tgtcggccta ctgctattac gaaggtaacc gctacaccga ggccatcgac gcttcgacgc
    20221 ggtttatcca gctgcatccc ggcaacaagg atgtgccata cgcttattat ctgaaagcga
    20281 tctcgctcta tgagcagatc ggcgatgtgg aacgcgatca gggcaagacg gatgaggcgc
    20341 ttaccgcgct gcaggatctg atccaacggt ttccggacac ggaatatgcc cgcgatgcac
    20401 ggttgaaggt ggatctcgca cgcgaccacc tagccggccg cgagatgaca atcggccgct
    20461 tctatctgca tgaaggcgaa tatctggccg cgatcaatcg ctttcgcaca gtgatcgata
    20521 aatatcagac cacgacgcac gcacccgaag cccttgaacg cttgaccgaa gcctacctgt
    20581 ccttgggcgt ggtgaaggag gcgcaaacct ctgccgccgt gctcggctac aattacccgg
    20641 gcagcgcctg gtacatggat gcctatactc ttttgaccga ggaaaatatc gctccggccg
    20701 aagaccagga atcctggatc agcagggcgt atcgggcggt gttctgataa aagcaggtca
    20761 tgctggcctc gctcaccgtt cgcgacatcg ttctgatcga aaaggcggag cttcacttcg
    20821 cgccgggctt gaatgtcctg accggcgaaa cgggtgcagg caaatccatt ctgctggatg
    20881 cccttagtct
//
LOCUS       DASYEG010000109        32219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_32854, whole genome shotgun sequence.
ACCESSION   DASYEG010000109 DASYEG010000000
VERSION     DASYEG010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 32219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32219
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32854"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(938..1013)
                     /locus_tag="VGM72_04210"
     tRNA            complement(938..1013)
                     /locus_tag="VGM72_04210"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(977..979),aa:Glu,seq:ctc)
     gene            complement(1073..1498)
                     /locus_tag="VGM72_04215"
     CDS             complement(1073..1498)
                     /locus_tag="VGM72_04215"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEY4264497.1"
                     /translation="MGRSLHLPDALAAQILNAAARSYPCECCGLIEGVSREEGWHVTA
                     VHEIANVAENPTRHFQIDPQAQFDLMRTLRGSQRRIIGCFHSHPDGVPAPSATDRAQA
                     YESDFLWLIVGGSTEGGLTLGAHHFVEETGFAPIAVRED"
     gene            complement(1498..1812)
                     /locus_tag="VGM72_04220"
     CDS             complement(1498..1812)
                     /locus_tag="VGM72_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264498.1"
                     /translation="MATSDAPNVPSHRGLLAVVIILGVLILLGVVGLMVGAVLKAGNR
                     APADASYAATVVAPGEHVESTELDGSRILLRLSGPNGEELVVLDAGSGRVLGRIAINA
                     KP"
     gene            1841..2833
                     /locus_tag="VGM72_04225"
     CDS             1841..2833
                     /locus_tag="VGM72_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY4264499.1"
                     /translation="MHEFRASETHKGWRLDHFLAANLPELSRSRLQDLIAKDAVTLGG
                     TTIKDPNKRVKPEDLIAIFVPPPLPASPQPQSIPLAIVYEDSDLLVIDKPAGLVVHPA
                     AGNLDGTLVNALLAHCGDSLSGIGGVARPGIVHRLDKDTSGLLVAAKTERAMRSLAKQ
                     FAAHAVERAYNAVVWGAPRVGEGLVEGNLGRNPFDRKRMAVLRGQGKPARTRYRVLEK
                     FGSDEKPFAALVECRLETGRTHQIRVHLAHIGHPLIGDSTYGRSRHPPRAKTPIEKTA
                     YEAADEFPRQALHAGVLGFFHPTRQLKLRFESAWPDDFSSLVKALRGLTLTGAP"
     gene            2970..3860
                     /gene="rpoH"
                     /locus_tag="VGM72_04230"
     CDS             2970..3860
                     /gene="rpoH"
                     /locus_tag="VGM72_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEY4264500.1"
                     /translation="MATRALPAMGEGGLSRYLAEIRKFPLLDPQEEYMLAKRWREHED
                     TDAAHRLVTSHLRLVAKIAMGYRGYGLPINEVIAEGNIGLMQAVKRFEPERGFRLATY
                     AMWWIRASIQEYILRSWSLVKLGTTAAQKKLFFNLRRVKGQIEALEEGDLRPEHVTDI
                     AKKLGVPEEEVVNMNRRLASPDSSLNTPLGADSDMEWQDWLPDDSVDQETRLAETEEL
                     DERRTLLSTALEELTPRERDILEARRLRDEPMTLEDLSVKYDISRERVRQIEVRAFEK
                     LQEKMKSALSHKRAETLDAR"
     gene            3922..4476
                     /locus_tag="VGM72_04235"
     CDS             3922..4476
                     /locus_tag="VGM72_04235"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY4264501.1"
                     /translation="MQKLINTLVLGVAGIFVVSAAFAIVQQMVKTMTLQSLSANDISV
                     DQALLVAQGAINECKKDSPALTVAVVDHEGGVRFLIRGDGASPEAVENARKKAYTART
                     FRASTSSWMERTAMDALDDKGKRIDLYGQRMLENIIAQKGGLPIIWHGDAIGGVGVSG
                     SKGGEETDEGCAKAGVEAIADQLF"
     gene            4524..5042
                     /locus_tag="VGM72_04240"
     CDS             4524..5042
                     /locus_tag="VGM72_04240"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY4264502.1"
                     /translation="MSRMSTIWKAAAAAALLSIASQSAYAQSSSLTSAQAMAALQAVQ
                     AYAKNDGSNPSLAVIDRDGNTVLLLKGNGAAPHNIGLAQRKAFTANQFKMRSIDWRDR
                     TMPGKPNADERNTPNAVPLGGGVPIMVGNDVVGAVGVSGTRGGQEGDTAGAQAGADAA
                     AAAAAQRPLAQN"
     gene            5063..5758
                     /locus_tag="VGM72_04245"
     CDS             5063..5758
                     /locus_tag="VGM72_04245"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY4264503.1"
                     /translation="MKALFSLLFCALAVLGVTQANAADVVIQGPIHTTTFVEVSPGAV
                     TQTVAMLKEYRDATRKEPGATSANIYQEATTPSRFVSNEVWKDMAAYEAHAKATAATQ
                     LAQKLHPIEYGPPDSRTHIGYFMAPGGGAPTANSVVILSHLDVTPNVLPKLMEIMKSL
                     SEGSAKDPGMQTYQILRQTAGAGNHFRLFEIWASDRAFDAHNLAAHTQGFRDELYPLL
                     GTPYDQRKYVPVN"
     gene            5823..6533
                     /locus_tag="VGM72_04250"
     CDS             5823..6533
                     /locus_tag="VGM72_04250"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY4264504.1"
                     /translation="MRSVHRLIACAVLAAALIGPAQGQQQIQGVTVVNGPIYTMTYIE
                     VIPSAKAQAIAMMKDYRDASRKEPGAMYADIYQEEGQSHRFVVSEIWQNRGAVETHTK
                     GAAVAGLIQKMKPIETGPLDMRIHQAHSVTPPHAPNANDVVVISHIDVAGGNTQNLIN
                     AFAPLAEGSRKEPGMVQFEILDEVPAHVNHFRSFEEWSSLAAFEAHNRAPHTQAYRTT
                     VLQWLGTPYDQRLYRLVN"
     gene            6563..6946
                     /locus_tag="VGM72_04255"
     CDS             6563..6946
                     /locus_tag="VGM72_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4264505.1"
                     /translation="MIDHIGIPVSDYACAKAFYEKALAPLDYRLIMEVQQPEDGIPAA
                     GFGADSKPDFWIGGEGGLNKRVHIAILAKSRSMVDAFYKAALAAGGRDNGAPGLRPLY
                     HPNYYGAFVLDPDGHNIEAVCHDAE"
     gene            complement(7046..7840)
                     /locus_tag="VGM72_04260"
     CDS             complement(7046..7840)
                     /locus_tag="VGM72_04260"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4264506.1"
                     /translation="MKNIRLLGAAAAVAVLALASTTLSVSMSTSVAAQTAQDDKLATS
                     NGDLVIHPVHHAGVVLTWNGKRIVADPTAFPPDPMKSGAADFKGAAAPDLILITHEHD
                     DHYSVPTLTDLAGPNTVIVAPQSVFAKMPPALQAKTKVMKNGDKGNFAGVPVEAVVEY
                     NTTPERMMYHPKGRDNGYVLTMGGKRIYLAGDTEEAPELKNLPNIDVAFVPMNLPYTM
                     TEEAAANWVKDFKPKIVYPYHYGMSDVKKFAMLVGNASEVRLRKWY"
     gene            7979..8812
                     /locus_tag="VGM72_04265"
     CDS             7979..8812
                     /locus_tag="VGM72_04265"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEY4264507.1"
                     /translation="MKLNLWQIDAFSEKPFRGNPAAVVPLRQWLPDITMQAIANENNL
                     AETAFFVPRELGFYDLRWFTPTMEVPLCGHATIASAWVIFSELAPELDCVRFATQSGV
                     LTVEKGPDGCHRMAMPAGRVESWIGPEGLSHALGESLGVGPPSEIHFAPTGAGGTPAP
                     LAVWPEAAIRSMQYSGALSAALSDAGLDALLVTARGDGAPYDYVARFFAPGLGVPEDP
                     VTGSMNCTLVPFWAKRLGKTQLRAYQASPRGGDLLCTDEGERVVLSGPCALYLRGEIE
                     V"
     gene            complement(8785..9120)
                     /locus_tag="VGM72_04270"
     CDS             complement(8785..9120)
                     /locus_tag="VGM72_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264508.1"
                     /translation="MNDYSATDPILTAWAEKHGLHVYTGHRQNVVRSITVYVWADARH
                     ESTGHIWLDPPNELGLVGLHAANRGFRLDEAVPIAGLEAGLDAVCERLAAQNRHAGRT
                     DQTSISPLR"
     gene            9254..10411
                     /locus_tag="VGM72_04275"
     CDS             9254..10411
                     /locus_tag="VGM72_04275"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF002841.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEY4264509.1"
                     /translation="MTGNYPAVRPARLFFSSGPCTKRPGWTLEALNQACLGRSHRSKP
                     GKAKLKEAIDRTRSVLCVPADYRIGIVAGSDTGAVEMALWSLLGPRAIDVLAWESFGE
                     DWVTDVAKQLKLKDTRIVKAAYGELPDLRQIDGARDVVFLWNGTTSGVRVPNGDWIAD
                     NRQGLTICDATSAAFAMDLPWPKLDVVTFSWQKVLGGEGAHGMLILSPRAVQRLETYI
                     PPWPMPKTFRMTKDGKLVEGIFEGETINTPSMLAVEDYLDTLKWAESIGGLSGLMARA
                     DRNLGVLANWVQKTGWVDFLARDPLTRSNTSVCLKVVDPWFTALNAEAQSAAASRIAS
                     LLEKEGVALDIGGYRAAPPGLRIWCGATVESSDLEALTPWLDWAFAHVKNG"
     gene            10418..11995
                     /gene="serA"
                     /locus_tag="VGM72_04280"
     CDS             10418..11995
                     /gene="serA"
                     /locus_tag="VGM72_04280"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEY4264510.1"
                     /translation="MPKVLISDKLSPAAIAIFKERGVEADVKTGLAKDELIKIIGDYD
                     GLAIRSATKVTADVIRAAKKLKVIGRAGIGVDNVDIPAATAAGMIVMNTPFGNSITTA
                     EHAIALMMALARDIPAANASTHAGKWEKNRFMGVELTGKVFGLVGCGNIGSIVADRAK
                     GLRMRVIAYDPYLSPERATDLGVEKVELNDLIARADFISLHTPLTPETRNIISADAIA
                     RMKKGARLINCARGGLVDEAAVKAALDSGQLAGAAFDVFEEEPATSNLLFGNEKVVST
                     PHLGASTSEAQENVALQVAEQISDYLLTGAVTNALNMPSISADEAQKVGPWIALAEHL
                     GAFAGQLTESSISAVELVFEGTASTLNTRALTQAAISGLLKPALSEVNMVNAPIVAKE
                     RGVRISEVRRDQEGAYEGYIKLTVTTDQQARSVAGTVFSNNRPRLIQIKNIDLDAEFA
                     PHMLYITNEDKPGFIGRLGMLLGNAHVNIANFNLGRASRGADAICVVQVDERVPDSVL
                     QEIRSLPWVKQARALAF"
     gene            12032..12595
                     /locus_tag="VGM72_04285"
     CDS             12032..12595
                     /locus_tag="VGM72_04285"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY4264511.1"
                     /translation="MSPALGFILAWTAWALSWGAAAAWSSHTAKRSAIASALAYRLTI
                     TAGAVLLFHRTSERLKAPMLWHVGLIGAWLLVAVAVLGFLFTWWARLHLGKLWSGSVT
                     LKEDHRIIDSGPYGLVRHPIYTGILVAIFATAAAEATLPAMTGAALMSLGIWMKARVE
                     EKFLRQELGAAAYDSYAARVPMLLPFL"
     gene            12662..13957
                     /locus_tag="VGM72_04290"
     CDS             12662..13957
                     /locus_tag="VGM72_04290"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEY4264512.1"
                     /translation="MANVAVIGAQWGDEGKGKIVDWLSARADVVVRFQGGHNAGHTLV
                     IDGVTYKLSLLPSGVVRPGKLAIIGNGVVVDPWAFLEEIARVQALGVAVTPDNLIVAE
                     NAALILPLHRELDVARESDSSVQALGTTKRGIGPAYEDKVGRRALRVVDLKDFANLPR
                     KIDRLLAHHNMLRRGGNLPEIDARALFDSLRDIAPKIVPFASCAWRRLDEVRKQGKRI
                     LFEGAQAVLLDIDHGTYPYVTSSNTVAGQAAAGSGIGPRHIGYVLGIAKAYTTRVGEG
                     PFPTELTDATGERIGKIGAEFGTVTGRKRRCGWIDTVLVRQTVITGGIDGIALTKLDV
                     LDGFDEIKLCIGYRLDGRQIDYLPADATEQARCEPVYESMDGWKGSTRGARSWVQLPA
                     QAVKYVRRVEELIGAPVALLSTSPEREDTILVRDPFIDR"
     gene            complement(14239..15027)
                     /gene="thiD"
                     /locus_tag="VGM72_04295"
     CDS             complement(14239..15027)
                     /gene="thiD"
                     /locus_tag="VGM72_04295"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEY4264513.1"
                     /translation="MIEPARVLIVAGSDSGGGAGIQADIKTVTALGGYAMTAVTAITV
                     QDTTGVHGFHPVPPGIVADQMRVVLADIGADAIKTGMLVSAACVEAVAAALAAYASVP
                     LVVDTVMIAKGGHALLDDAGVDAMRRLLFPLAALITPNAPEAARLTGIAVRSPEDSAR
                     AGMILRDAGARAVLMKGGHISGDLVTDVLVDDEGVHQFTAPRIANPSTHGTGCTLASA
                     IAVGLAQGLPLRDAVIRARNFVQEAIRNGLAFGHGTGPVNPHAR"
     gene            complement(15024..16370)
                     /gene="glmM"
                     /locus_tag="VGM72_04300"
     CDS             complement(15024..16370)
                     /gene="glmM"
                     /locus_tag="VGM72_04300"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF008139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY4264514.1"
                     /translation="MTRKMFGTDGIRGLANKFPMTSEIALKVGMAAGRIFTRGDHRHR
                     VVIGKDTRLSGYMIESALVSGFTSVGMDVFQFGPLPTPAVAMLTRSLRADLGVMISAS
                     HNAYHDNGIKLFGPNGQKLSDEREHEIEALMENGLEDGLAPPENIGRARRIEDAQARY
                     IEFVKRTFPKHLRLEGLRIVVDCAHGAAYRVAPAVLWELGADVFAIGVDPNGMNINQE
                     VGSTAPTAMIEKVKETRADFGIALDGDADRVVMCDEKGYIIDGDQVLALIARSWAKSG
                     ELKGGGVVGTVMSNIGLERYLTTQGLKLVRSPVGDRYVIEKMRAGGFNVGGEQSGHII
                     FSDFITTGDGLIGALQVLAVLAEERRPASEAAHIFEPAPQVLENVRLKRGEPLSDATV
                     ASHIKACEARLNGHGRLLVRKSGTEPVIRIMGEGEDEKLVRQIVREVAQVIREASA"
     gene            complement(16459..17262)
                     /gene="folP"
                     /locus_tag="VGM72_04305"
     CDS             complement(16459..17262)
                     /gene="folP"
                     /locus_tag="VGM72_04305"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY4264515.1"
                     /translation="MTTIFGILNITADSFSDGGRFLDPEAALAHAVKLIADGADALDL
                     GAASSNPRSEPVSPDIEIGRLAPIVLAAKERGWKISVDSFASETQLWALDEEVDYLND
                     IQGFADASLYAHLAKARAKLVVMHAVQETGRAQRIDTDPRTIVGRIADFFEHRIDALT
                     HAGIARERLILDPGMGLFVGTKPEVSLTILRRLSQLKSAFGLPILVSVSRKSFLRKLA
                     GRSVEEIGPGSLAAELYAALHGADIIRTHDPKSLRDALTIWSHIATGIG"
     gene            complement(17294..19222)
                     /gene="ftsH"
                     /locus_tag="VGM72_04310"
     CDS             complement(17294..19222)
                     /gene="ftsH"
                     /locus_tag="VGM72_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY4264516.1"
                     /translation="MNNFRNLALWIIIALLLVALFNLFQGPGSRQGARTPISFSEFNQ
                     QIVADGIKTVNIAGTHVEGQFKNGTPFSTDIPDDPGLTSRLLEHNVDIKVRPEGDNIT
                     LLSVFINWFPMLLFVAVMIFLMRQMQAGGGKAMGFGKSRAKMLTERQGRVTFEDVAGV
                     DEAKEDLVEIVDFLKDPQKFQKLGGRIPKGVLLVGPPGTGKTLLARAIAGEANVPFFT
                     ISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIVFIDEIDAVGRHRGAGLGGGNDEREQ
                     TLNQLLVEMDGFEANEGVILIAATNRPDVLDPALLRPGRFDRQIVVPNPDLSGREKIL
                     KVHMRKVPLSPDVDARVIARGTPGFSGADLANLVNEAALLAARRGKRLVSRAEFEDAK
                     DKVMMGAERRTLAMTDEEKKLTAYHEGGHALVALTVVGSDPIHKATIIPRGRALGMVM
                     RLPERDQVSLTRQKLYADLCVAFGGRIAEELIFGYDKVTTGAQSDIEMATRMARAMVT
                     RFGMSDELGPISYGENQEEVFLGHSVSRTQSVSEATAQKIDSEVRRIIEETYNRAKQI
                     LSDRIEDLHMLAKGLLEYETLTGDELKGLMRGERPVRTPYEEPESQTQPRPAATVPQA
                     GRAREPGITISPEPQPGT"
     gene            complement(19340..20410)
                     /gene="tilS"
                     /locus_tag="VGM72_04315"
     CDS             complement(19340..20410)
                     /gene="tilS"
                     /locus_tag="VGM72_04315"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEY4264517.1"
                     /translation="MPGFAAALAVSGGGDSIALMHLFADWARRESISRPAVLIVDHGL
                     RRESYDEAALVAQWAKNAGFAAAVMRWRGRKPQSNIEDEARAARYRLLGEWCKARMTP
                     YLFAAHTREDQAETFLLRLGRGSGVDGLSGMRAAGPLPVPGFAPVRLLRPLLDMGRTE
                     LRAYLQGRGEHWLEDPMNADCRFARVRIRQALPGLEAAGVSVQRIAQAAGHLARAREA
                     LEAATEEFFERHTQLGADFALMDGGALAHNSREIGLRVLSAVLMRVAGAPYRPRFERL
                     EALFDAISVEPFTARTLLGCRIAKAPKGRAVFGGATLLIVREPARRSRLNGANAGKLM
                     RNADTVSDRPGNPPHKGRIRPL"
     gene            complement(20466..21491)
                     /gene="ybgF"
                     /locus_tag="VGM72_04320"
     CDS             complement(20466..21491)
                     /gene="ybgF"
                     /locus_tag="VGM72_04320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEY4264518.1"
                     /translation="MKAMRGRWMKAGAALVGGFVWVSGAGVSGASAAPATQVIDARDL
                     MARLQRLERDMHDVQAETFRRSGGTGPAPAPVEASPGLIPAPIPDAQTQQPPVPDLNP
                     LMRRLGDLEDGLGRLTGQMEEIGHQVDELSQKTDRLQKQLEYQAAQQTAPVANTDTPS
                     ATAGLPPLPAGSGNAVASLTPDQNLSPLRGAPPSNLGQIPAGTAIPAAPAPSASPKAE
                     FDTAMNLLSRAQYEPASAAFRKFADAHPDEDRAPDALYWTGDIAYSARKDYPEAARDF
                     AELLKKYPKSQRAPEGMLKLGLALFGLGQMKEGCAAIAALPAKYPDASPAVATRAKSE
                     RATNKCK"
     gene            complement(21657..22220)
                     /gene="pal"
                     /locus_tag="VGM72_04325"
     CDS             complement(21657..22220)
                     /gene="pal"
                     /locus_tag="VGM72_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEY4264519.1"
                     /translation="MTKLTTSAKLASLSFVLMLTACADKMMEAPPPAPPPPPAATPAP
                     PPPPPAPPPRPPAPVVQSIVPGSVQDFQVNVGDRVFFEYDKTDLTEAGRATLQKQAAW
                     LQRFGAVTLTLEGHCDERGTREYNLGLGARRAQAEKDYLASLGVSAARLDTISYGKER
                     PVCVESTEACWAQNRRGVSTIKSGSVS"
     gene            complement(22352..23698)
                     /gene="tolB"
                     /locus_tag="VGM72_04330"
     CDS             complement(22352..23698)
                     /gene="tolB"
                     /locus_tag="VGM72_04330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEY4264520.1"
                     /translation="MHAQNSFRHLSRHFILAAVLGLGMVAGVFEPANAALQVRVDQGV
                     TEPIPLAIPDFLGTGQAGRDIAGVVRGDLERSGLFRSLNPASFIEKITDINTPPRFGD
                     WRTIQAQGLVTGQAVMQPDGRLKVDFRLWDIFGESQMVGLQFMTTPENWRRIAHLVSD
                     AIYERITGEKGYFDTRIVFVAESGPKLNRRKRLAVMDQDGANPVFLTQGDYLVLTPRF
                     NPTAQMIAYMSYIQSRPRVYLFDLETGRQEMLGNFPNMTFSPRFSPDGKSVVMALETN
                     GNSEIYVMDLATRTSRRLTNDPAIDTAPSFSPDGKQITFESNRGGSQQIYVMNADGTN
                     VHRISFGQGKNGTPVWSPRGDLIAFTNMTPSYFGVGVMRPDGSGTRLLTHGWQDEGPT
                     WAPNGRVIMFTRTLQTGAGGAGGGSQIWSVDITGRNERRVLSPGDASDPAWSPLIQ"
     gene            complement(23735..24559)
                     /locus_tag="VGM72_04335"
     CDS             complement(23735..24559)
                     /locus_tag="VGM72_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264521.1"
                     /translation="MLKRSIVVSGTLHLAVIVAATVAWPHALNLSDESPPVVPVDLVT
                     IADVTNIAPTVQETKKVETPPDTPAPPQPEVAAPPPPEAEVAPPDLDKTPTPKPKELP
                     KEAEQAAPAPAKPALPRRKPQATPQAKFDVDSVIALLDKRAPKPEAPPANAKLAEQTV
                     KGLGDQNALTMDIKDALLAQMRECWNVPAGAPNPEQLIVQVRVFLAPDGRLLQPPQLE
                     PATRAAAATNQYMRTAAEAASRAINVCAPYKRLPPDKYDTWREIVMTFDPSKMTGH"
     gene            complement(24657..25130)
                     /gene="tolR"
                     /locus_tag="VGM72_04340"
     CDS             complement(24657..25130)
                     /gene="tolR"
                     /locus_tag="VGM72_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HEY4264522.1"
                     /translation="MPAVMNNPRAGRGRRGGGRMRGGRPMSEINVTPFVDVMLVLLIV
                     FMVTAPLLTVGVPVDLPQTRAKSLGEDREPLAVSIDNGGKIFLQNTPIGIDDLVPKLM
                     AISNNGYDQRIFVRGDKAVDYGKVMEVMGLLNSAGFTRIGLVTDAQKKPPQRPLK"
     gene            complement(25130..26026)
                     /gene="tolQ"
                     /locus_tag="VGM72_04345"
     CDS             complement(25130..26026)
                     /gene="tolQ"
                     /locus_tag="VGM72_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008034890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEY4264523.1"
                     /translation="MRLAAITLMALLLCVTLDGANHSAFAQFQRAAPSGGRPPSAAPS
                     GTPAESAPLAAPSGIADANVAGSQAEIQEAFTPVGLFLRADIVVKGVLVLLLLASFWS
                     WVLIVDKLIAFATLKRKAKRFEKSFWSGRSLEELYAQHQQRTDQPFTAVFVAALREWK
                     RSFEGGTPPETQLANVKDRVEKAMNISIQRELEKIERGLGYLATVGSTAPFVGLFGTV
                     WGIMTAFTAIAARNDTTLAVVAPGIADALFATALGLVAAIPAVIAYNRFVNELGRYTG
                     RLEIFADEFSTFLSRQLDEKAR"
     gene            complement(26264..27058)
                     /locus_tag="VGM72_04350"
     CDS             complement(26264..27058)
                     /locus_tag="VGM72_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY4264524.1"
                     /translation="MRKCFFVPALLLLLGATPALSLSDTSYLAPGAFDSVHLLPPPPR
                     ATSPQQGQDIDGVLRAQQTASPERIKRAEEDAKVDIAQFAAVLGTKFSFTAVPGVAAF
                     FRKVARDTREPVNMAKDCWERPRPFVADDRVHPPGAMREETANRPGATPANSAPHDRA
                     SPCLPVESPSPEFSYSYPSGHSTFGAMTAILLANMVPEKRNELFVRGWDYGQSRIIGG
                     VHFPTDVESGRIEATAMVALMMQNTDFRTDFAAAKAELRKALGLSE"
     gene            complement(27125..27847)
                     /locus_tag="VGM72_04355"
     CDS             complement(27125..27847)
                     /locus_tag="VGM72_04355"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY4264525.1"
                     /translation="MRKLALAPILFLSLLFLSLAASPVLALSDKPYIAATDVDFPALL
                     PPPPKEDSGAGKRDLQAILDLQKNMTPERMANIQRDLEQNVYVVAAPVLGPKFTKENF
                     PLAGEFFAKVTKDAGIGIGPIKQKYKKLRPFQYSKDVKTPENIAKVSGGPTYPSGHST
                     TGAEVAILMSMMVPEKREALYARGWGYGVNRMASGAAYPSDWESGHITASLAVNQMLK
                     NPEFRTDFEAVKAEIRKGLSLN"
     gene            complement(27874..28344)
                     /gene="ybgC"
                     /locus_tag="VGM72_04360"
     CDS             complement(27874..28344)
                     /gene="ybgC"
                     /locus_tag="VGM72_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEY4264526.1"
                     /translation="MTNPDPSTSPPGLSSGWLEGNIHVFPVRVYYEDTDFSGVVYHAN
                     YLRFMERGRSEFLRLAGAGHQGMLENAEPLVWAVRRMATDFVKPARMDDALSVHTEVL
                     ALAGARMRLAQSVMRAGLALVKADVEVCVITLDGRPRRVPDSTRKKLEIFLKSP"
     gene            complement(28331..29404)
                     /gene="ruvB"
                     /locus_tag="VGM72_04365"
     CDS             complement(28331..29404)
                     /gene="ruvB"
                     /locus_tag="VGM72_04365"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008034894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEY4264527.1"
                     /translation="MASPKSKQHPAKDGTRVVAPERSDDDELESTLRPKRLAEFTGQK
                     QARENLSVFIEAAKARGEPLDHVLFSGPPGLGKTTLAQIVARELGVGFRATSGPVLAR
                     AGDLAALLTNLEPRDVLFIDEIHRLNPAVEEILYPAMEDFELDLIIGEGPSARSVKIG
                     LAPFTLIGATTRSGLLTTPLRDRFGIPIRLAFYETNELMLIVARGARVLGLNLTQDGA
                     REIAMRSRGTPRIAGRLLKRVRDFAAVAGRVEVDQMVADAALARLEVDKRGLDAFDRR
                     YLALIAEGFNGGPVGIETLAAALGEPRDAIEEIVEPYLIQQSLVQRTPRGRVLTGQAY
                     VHLGLPGPSTPPGQPQLFGDDES"
     gene            complement(29412..30032)
                     /gene="ruvA"
                     /locus_tag="VGM72_04370"
     CDS             complement(29412..30032)
                     /gene="ruvA"
                     /locus_tag="VGM72_04370"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY4264528.1"
                     /translation="MIGKLTGRVDFLGTDHAIIDVGGVGYVVQCASSTLSRLSAGASA
                     TLWVETKMSDEAIRLYGFLAGDEREWFRLLQTVQGVGARVALSILSALRIAELERAIV
                     LGDKSVVGRAQGVGPKLATRIVTELKDKAPALALQRSAETGERVGGLQAPHPVESDAI
                     AALMKLGYSQMAAAEAIARASQNIGPNAEALPLDVLLRESLRQLAR"
     gene            complement(30029..30553)
                     /gene="ruvC"
                     /locus_tag="VGM72_04375"
     CDS             complement(30029..30553)
                     /gene="ruvC"
                     /locus_tag="VGM72_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005851638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY4264529.1"
                     /translation="MTTSIRIAGFDPGLSRTGWGVIQVAGAKLTHVANGVIVTTTAHP
                     LGVRLAMLHAGIRAVLAAHTPHAVAVEQAFVAKDPLAALKLGHARAIALLAAAEAGLT
                     IGEYAANHIKKSVVGVGHADKTQVQAMVRRLLPAVQFETADAADALACAIAHAHLAGT
                     RARIAAALERQLAQ"
     gene            complement(30633..31952)
                     /locus_tag="VGM72_04380"
     CDS             complement(30633..31952)
                     /locus_tag="VGM72_04380"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEY4264530.1"
                     /translation="MRSLMRLFVLFLVLCVFGSSPVLADAEPETYSLHGQTTFVLQYH
                     PAFRSPYRGANSLDPGSRGNETFDATLFAGARLWQHTEAYIDIEADQGFGLSNTLGVA
                     GYLSGEAYKIGDSSPYLRIPRLFARQTFDLSGEDVSIAPAANQLGGSRLSDNVIVTLG
                     KFAVTDIFDTNMYAHDPRSDFLNWSVIDAGAFDYAADAWGYTYGGAAEWTQDWWTLRI
                     GAFDLSRVPNSRFLQRGFGQFEVTSELEARFTAGAHPGKIKLLAFANRGRMADYEKAV
                     ETARASGATPDVAAVRDYATRPGFALNIEQELGPGWGMFLRASANDGTKEAYEFTEIN
                     KSLSGGLSLRGTAWGRPDDALGLAGVVNGLSQPAQDYFAAGGLGILIGDGRLAHYGSE
                     DIVETYYRLAVREGLALSFDWQYVAHPAYNAERGPVLAFGFRVHAER"
     gene            complement(32004..>32219)
                     /locus_tag="VGM72_04385"
     CDS             complement(32004..>32219)
                     /locus_tag="VGM72_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY4264531.1"
                     /translation="SAVRDALEAKLGAPAQSAKIIWRPGNTVPVSDEAGETLSKLLDV
                     LEDHDDVQNVFANYEMSEALIEKLSAA"
BASE COUNT         6285 a        10018 c         9786 g         6130 t
ORIGIN      
        1 ctcttgcggc tcttcggcat caacggttca aggagcgtcc attcctcatc ggataaatca
       61 aatcgcatga tccacctccc atcacggtga atcactgttc gtcaaaattt tcaacgatct
      121 atgtgggtac agaccctagt ccgacttaag cacgaacttt tccgcatcga ttgaccaact
      181 tgaagtcatt tctgagccgc gcggacatgc ctttcgcgtg gccatctacg ttggccagca
      241 ccacgattca tggttatttt catccacatg atgtgcatgg accgcgtggg gctcgataag
      301 aacacttcgt tcctatggaa gtgttctcga atcgtctgcg tcaggaagca cgcatgcttg
      361 gcttagcgga cgtccaggtt gctcgtcggg caggacttac ggaacgacgc tacgggaatt
      421 atgttgcggg cattcgtgag cccgatctac ttatcttggt gaagataacc accgcgcttt
      481 ccaccacgcc caaccgactg cttggcattg gggatgcccc aggaacgctc gatgaaaaag
      541 ccacatacat cgctatatta agtgcggctg agaccctccc tgtcgaagac cttgaatccg
      601 tcctcattca gatagaggcg cttgcgcgtc acagggcgaa aaagccgaag cgcaggcgag
      661 acgaatatta gcggtggccg ccggggatat cggacttggc tgcggcgctt cggggggtag
      721 tacccctggc gtagcagcaa tgcctcgcct gaacgtttct aacccgttcg gcggccattt
      781 ctttattcct ggccggatgg cagcttggtc ttcggcgcta gggcttggct atcctatagc
      841 taaattgcag aagggactgc cttttgtggg gtaccagggg tggaaaatac cctgctaacg
      901 taagccgctg attttaaaaa taaagtttga tatggtttgg cgcccccatg gggtttcgaa
      961 ccccagtttt cgccgtgaga gggcgacgtc ctaggccgct agacgatggg ggcggcgaca
     1021 aaaccgctta tacggttgcg ctgggatcgg atcaagacga gcgcacgccc tctcaatctt
     1081 cgcgcacggc gattggggca aagccggtct cttccacgaa atgatgagca ccaagcgtga
     1141 gccccccctc tgtggaaccg cccacgatca gccacagaaa atcggattca taggcttggg
     1201 cacgatcggt cgcggatggc gcgggcaccc catcggggtg ggaatgaaag cagccgatga
     1261 tccgccgttg cgacccacgc agagtgcgca tcagatcgaa ctgcgcttgc ggatcgatct
     1321 gaaaatgccg cgttggattt tccgccacat tcgcgatttc atgcacagcc gtcacgtgcc
     1381 agccctcctc tctcgacacg ccctcgatca ggccgcaaca ttcgcaaggg tagctgcgag
     1441 cggcggcgtt cagaatttgc gccgccagcg catcgggaag atggagagac cgtcccatca
     1501 cggctttgcg ttgatcgcga tgcggcccag cacacgcccc gatcccgcat cgaggaccac
     1561 caattcctcg ccgttcggtc cggacagcct gagcaggatc cggctgccat cgagctctgt
     1621 cgattcgaca tgctcgcccg gcgccaccac cgtcgcggca taggaggcat cggccggtgc
     1681 gcggttgcct gccttgagca ccgcccccac catcagccca accacgccta gcaatatgag
     1741 cacgcccagg ataatgacga cggccaaaag gccgcgatgg cttgggacat tgggggcatc
     1801 gctcgtagcc atggccgatt cttcatcgtt aaacggaaag atgcacgaat ttcgcgcaag
     1861 cgaaacgcac aagggatggc ggctggacca tttcctggcc gccaatctgc ccgagctttc
     1921 acgttcacgc ctgcaggacc tgatcgcgaa agacgccgtc acgctcggcg gcacgacgat
     1981 aaaagacccg aacaagcggg tcaaaccgga ggatctgatc gcgattttcg tgcccccgcc
     2041 ccttcccgct tcgccgcaac cccaatccat tccgcttgcc atcgtctatg aagattccga
     2101 ccttctcgtg atcgacaaac cggcgggact cgtggtccat ccggcggccg gcaatctgga
     2161 cggcacgctc gtcaacgcgc tgcttgccca ttgcggagat tctctttccg gcattggcgg
     2221 tgtcgcacgg ccgggaatcg tgcaccggct cgacaaggat accagcgggc ttctggtcgc
     2281 cgccaagacc gagcgcgcga tgcgcagtct cgccaagcaa ttcgccgcgc acgccgttga
     2341 gcgcgcctac aacgccgtgg tctggggggc gccgcgggta ggcgagggtt tggtcgaggg
     2401 caatcttggc cgcaacccgt tcgaccgcaa acgcatggcg gtcctgcgtg gacaaggaaa
     2461 gccagcccgg acccggtatc gggttctgga aaaattcggc tctgacgaaa aacccttcgc
     2521 cgcactggtg gaatgccgcc tcgaaacggg gcggacgcat caaatccgcg tgcatctcgc
     2581 ccatatcggc catcccctga tcggggattc cacctatggc cgcagccgcc accccccgcg
     2641 ggcgaaaacg ccgatagaaa aaaccgccta tgaggccgca gatgaattcc cccgccaggc
     2701 gctgcatgcc ggcgtgctcg gtttcttcca tcccacgcgc cagctcaaac ttcgcttcga
     2761 atcggcttgg ccggatgatt tctccagcct ggttaaggcc ttacgcggat tgacacttac
     2821 aggcgcacct taggcgatct tgcaatacag ccaagccata cccacattcg gtagagatcg
     2881 gggatacgca caatccgaca tgcgttagca ccatggtagg ctatgctccg gcagagccgt
     2941 gtgtgcgtcg ttgtaagggg aattaggcaa tggcaacgag agccttaccg gccatgggcg
     3001 aaggcggcct ttcgcgctac ctcgctgaaa tccgcaaatt ccccctgctc gacccgcagg
     3061 aggaatacat gctcgccaaa cgttggcgcg agcatgagga caccgatgct gcgcacagac
     3121 tcgttaccag ccatctcaga ctggtggcga agatcgccat gggttatcgc ggctatggct
     3181 tgccgattaa cgaagtcatc gcggaaggca atatcgggct gatgcaggcg gtgaaacgct
     3241 ttgagcccga acgcggcttc cggctagcga cttatgcgat gtggtggata cgggcctcta
     3301 tccaggaata catcctgcgt tcctggagtc tggtgaaact cggtaccacg gcggcgcaga
     3361 aaaaactgtt cttcaatttg cgccgggtga agggccagat cgaagccttg gaagaaggcg
     3421 atctcaggcc tgaacacgtg acggacatcg caaaaaaact gggcgtgccg gaagaggagg
     3481 tggtgaacat gaaccgccgt cttgcctcgc ccgattcttc gttgaacacg cccttgggtg
     3541 cggactccga catggaatgg caggactggc tgccggacga ttccgtcgac caggagacac
     3601 gcctcgcgga aaccgaagaa ctggacgaac gccggacgct gctttccacc gccctcgagg
     3661 aactcacccc ccgcgagcgc gacattctcg aagcgcgccg gcttcgcgac gaacccatga
     3721 cgctggagga cctctccgtc aaatacgaca tcagccgcga gcgcgtgcgc cagatcgagg
     3781 tccgggcctt tgaaaagctt caggaaaaga tgaagtcggc gctctcccac aagcgcgccg
     3841 agaccctcga cgcccggtag tctctgccct aaactgcgcg ccggaacttg aaaaagaccg
     3901 tgcgccgcgg ggagggtcag catgcaaaaa ttgatcaaca cattggtctt gggtgtggcc
     3961 ggcattttcg tcgtgtcggc cgcttttgcc atcgtccagc agatggtgaa gaccatgacc
     4021 ttgcagtcgc tgtccgccaa cgacatctcc gtggaccagg cgcttctcgt cgcccaaggc
     4081 gcgatcaacg agtgcaaaaa ggacagccct gccttgaccg tcgcagtggt ggaccatgaa
     4141 ggcggcgtgc gctttctgat ccgcggcgat ggcgcctcgc cggaagccgt cgagaacgca
     4201 cgtaaaaagg cttacaccgc acgcactttc cgggcatcga cctcgtcctg gatggagcgc
     4261 acggcgatgg atgccctgga cgacaagggc aagcgcatcg atctttacgg ccagcgcatg
     4321 ctcgaaaaca tcatcgcgca aaagggcggc ctgccgatca tctggcatgg cgacgccatc
     4381 ggaggcgtcg gggtttcggg atcgaaaggc ggcgaggaga cggacgaagg ctgtgccaaa
     4441 gcgggcgttg aagccatcgc cgatcagctc ttttagacag ccggatttcg cggccgtctc
     4501 agcactatct cgggaggatt tcaatgtctc gcatgtctac gatctggaaa gcagccgcag
     4561 cagccgccct tctttcgatc gccagccaat ccgcgtacgc gcaatcctcg agcctgactt
     4621 cggctcaggc catggcagcg ctacaggccg tgcaggccta cgccaaaaac gacggctcta
     4681 acccgtcgct ggctgtgatc gaccgtgacg gcaataccgt cttgctgctc aagggcaatg
     4741 gcgcggcgcc ccacaatatc ggcctcgcac agcgtaaggc gttcactgcg aaccagttca
     4801 agatgcgatc gatcgactgg cgcgaccgca ccatgccggg aaaaccgaat gccgatgagc
     4861 gcaatacgcc caacgccgtt ccgctcggcg gcggcgtgcc catcatggtc ggcaacgatg
     4921 tggtcggcgc ggtaggcgtc tcgggcaccc gaggcggcca ggaaggcgac accgccggcg
     4981 ctcaggcggg cgccgatgca gcggccgcag ccgctgcgca gagaccgctc gcgcaaaatt
     5041 aaacccttat tcggagtgat gcatgaaagc gcttttcagc ctgctgttct gcgcgctcgc
     5101 cgtcctcggc gtgacgcaag ccaacgccgc cgatgtggtg atccagggac cgatccacac
     5161 gaccaccttc gtcgaggtga gccccggagc cgtcacgcag actgtggcga tgttgaagga
     5221 atatcgcgac gccacgcgta aggaaccggg cgcgacatcg gccaatatct atcaggaagc
     5281 gaccactccg tcgcggtttg tcagcaatga ggtgtggaag gatatggccg cctatgaggc
     5341 tcacgccaag gcaaccgccg cgacgcagct cgcccagaag ctgcacccca tcgaatatgg
     5401 gccgccggat tcgcgcaccc atatcggcta tttcatggcg ccgggtggtg gagcgcccac
     5461 cgcgaacagc gttgtcatct tgagccatct cgacgtgacg ccgaacgtgc tccccaagct
     5521 catggagatc atgaagtccc tgtcggaagg ttcggcaaag gatccgggga tgcagaccta
     5581 tcagatcctt cgccagacag ccggcgcggg taatcatttc cggctgttcg agatctgggc
     5641 cagcgaccgg gcgttcgatg cgcataattt ggcggcgcac acccaggggt tccgcgacga
     5701 gctgtatccg cttctcggta cgccctacga ccagcgcaaa tatgtaccgg tgaattgagc
     5761 ttgggcgcac gcgagacggc agttttgccc tgtggaagat ggaagcaatc gtggaggaca
     5821 atatgagatc ggttcaccgc ttgatcgcgt gcgccgtgct ggcggcggcg ctgataggcc
     5881 cggcacaggg ccagcagcag atccaagggg tgacggtggt caatgggccg atttacacca
     5941 tgacctatat cgaggtcatt ccgagtgcca aagcgcaagc catcgccatg atgaaggatt
     6001 atcgcgatgc gagccgaaag gagcctggcg cgatgtatgc cgatatctac caggaagaag
     6061 gccaatcgca tcgcttcgtg gtgagcgaga tttggcagaa ccgaggcgcg gtggagaccc
     6121 acaccaaggg cgcagccgtc gccggcctga tccagaagat gaaaccgatc gaaacaggcc
     6181 ccctcgacat gcgcattcat caagcccatt cggtgacgcc gccgcacgcg cccaatgcga
     6241 acgatgtcgt tgtcatcagc catatcgacg tggccggcgg caacacgcag aatctgatca
     6301 acgcattcgc cccccttgcg gaaggcagcc gcaaggaacc cggcatggtg cagtttgaga
     6361 tccttgatga ggtacccgct catgtgaacc atttccgctc gttcgaggaa tggtcgagtc
     6421 tcgccgcctt cgaggcgcac aatcgtgctc cgcacacgca ggcttaccgc accaccgttc
     6481 tgcaatggct cggaacgcct tacgatcagc ggctgtatcg gttggtcaat taggcgggcg
     6541 atctgacaaa caaacggtcg ccatgatcga ccatatcggc attccggttt ccgattacgc
     6601 ctgcgccaag gcgttctacg agaaggcgct cgcgccgcta gactacagac tcatcatgga
     6661 agtacagcag cctgaggatg gcatccccgc tgcgggcttt ggagcggaca gcaagccgga
     6721 tttctggatc ggcggcgaag gcggtctgaa caaacgcgtg catattgcca tcctggcgaa
     6781 aagccggtcc atggtcgatg cattctacaa agctgcgctg gccgccggag gccgcgacaa
     6841 cggcgcaccc ggcctcaggc ccctctatca tccgaattat tatggcgcgt tcgtactcga
     6901 tcccgacggg cataatatcg aagccgtatg tcacgatgca gaataactga cacattcccc
     6961 aaaaggaacg gccgctttcc ccagggatag cggccgtttt gtctcctgtc atccttcgtc
     7021 aagcttggga tgagtcggta tcagatcaat accatttgcg cagacgcact tcgctcgcat
     7081 tgccgacaag catcgcgaat ttcttcacgt cgctcatgcc gtaatgatag ggatagacga
     7141 tcttcggctt gaaatccttt acccaattcg ctgccgcctc ttcggtcatg gtgtagggca
     7201 ggttcatcgg cacgaacgcg acatcgatat tgggaaggtt cttcaattcc ggtgcttcct
     7261 cggtatctcc cgcgagatag atgcgtttgc cgcccatcgt aagcacatag ccattatcgc
     7321 gccccttggg gtggtacatc atccgctcag gggtcgtgtt gtattccaca acggcttcga
     7381 ctggtacgcc ggcaaagttg cccttgtcgc cgttcttcat caccttggtc ttcgcctgca
     7441 gggccggtgg cattttggca aagacgcttt gcggcgccac gatcacggtg ttgggaccgg
     7501 ccagatcggt cagcgtcggc acgctgtaat ggtcgtcatg ctcgtgagtg atcagaatga
     7561 ggtcgggggc agcggcaccc ttgaaatcgg ctgcgccgct cttcatcggg tcgggcggaa
     7621 aagctgtcgg atcggcaacg atccgcttgc cgttccatgt cagtaccacg cccgcgtggt
     7681 gcaccggatg gatgaccaga tcgccgttcg aggtggcgag cttgtcgtct tgcgcggtct
     7741 gagccgcaac cgatgtggac atcgataccg aaagcgtcgt cgatgcgagc gccaacacgg
     7801 ccaccgcagc ggctgcgcct aaaaggcgaa tgtttttcac gattgtcttc tccaaggttc
     7861 ggtttcaaga agtcagcccg gcgatgcaca gcggccggcc ggggccgaag cgggttatac
     7921 tgcgatgatg caggttaggc tatggtcggc actatgcccc ggccatagaa ggggcacgat
     7981 gaaactcaat ctctggcaga tcgacgcatt ttccgagaag ccctttcggg gaaatcccgc
     8041 cgctgtggtg cctctgcggc aatggctgcc cgacataacg atgcaggcca tcgccaatga
     8101 gaacaatctc gctgagaccg ctttcttcgt cccgcgcgaa ttgggattct acgatctgcg
     8161 ctggttcacg cccaccatgg aagtgccgct ttgcgggcac gcgacaattg catcggcatg
     8221 ggtgattttc tccgaattgg caccggaatt ggattgcgtg cgcttcgcta cgcaaagcgg
     8281 ggtgctgacc gtggagaagg gaccggatgg ctgccaccgc atggctatgc cagcggggcg
     8341 cgtggaatcg tggataggcc cggaaggact cagccatgcg ctcggcgaat cgctcggtgt
     8401 tggtccaccc agcgaaatcc acttcgcgcc gaccggcgct ggcgggaccc ccgctccgct
     8461 cgctgtctgg ccggaagcgg ccatccgatc gatgcaatat tccggcgcac tctcggcagc
     8521 gctttccgat gccggcttgg acgcgttgct cgtgaccgcc cggggcgatg gcgcacctta
     8581 cgattacgtc gcgcgattct ttgcgcctgg cctaggcgtg cctgaagacc ccgtgacggg
     8641 ttcgatgaat tgcacgctcg tgcctttctg ggcgaagcgc ctcggcaaga cgcaacttcg
     8701 cgcctatcaa gcatccccgc gtggtggcga tcttctgtgt acggatgagg gtgagcgtgt
     8761 ggttctgtcc ggaccctgcg cgctttatct caggggcgag atcgaagtct gatccgtgcg
     8821 accggcgtgt ctgttctgcg cggccaggcg ctcgcacacg gcatcgagac ccgcttcaag
     8881 accggcgata ggcaccgcct cgtccagacg aaagccgcgg ttggcggcat gaagtccgac
     8941 gagtcccaac tcgttcggcg gatcgagcca gatatgtccc gtcgattcgt gccgcgcatc
     9001 ggcccagaca tagaccgtga tcgagcgcac gacgttctga cgatgaccgg tatagacatg
     9061 caacccgtgc ttttcggccc aggccgtcag aatgggatcg gttgcgctat aatcattcat
     9121 ggcgacatgt tgagcaaagc caccgcaaac ggcaagcttc caacgcgcga ttgacgcgag
     9181 cgtgccgact cgctacagac gcctcgggcc acgctccccc aacggagcgc catcattctg
     9241 gaaggaatga aacatgaccg ggaattatcc ggccgtgcgc ccagcacggc tttttttttc
     9301 gtccggacct tgtaccaaac ggccgggctg gacccttgaa gccctgaacc aggcgtgcct
     9361 gggccgctcg catcgctcca agccgggtaa agcaaagctc aaggaagcca tcgaccgaac
     9421 ccgcagcgtg ctatgtgtcc ccgcggacta tcgcatcggc atcgtcgcgg gttccgatac
     9481 gggtgccgtg gagatggcgc tttggtcgct tctgggaccc cgcgcaatcg acgttctggc
     9541 ctgggaaagc ttcggcgagg actgggtcac cgatgtcgcc aaacagctca aattgaaaga
     9601 cacgcgcatc gtcaaggcgg cctatggcga attgccggat ctgcggcaaa tcgacggagc
     9661 ccgcgatgtc gtcttcctgt ggaatggaac gacatccggc gtgcgtgtgc cgaatggcga
     9721 ttggatcgcc gataaccgcc aaggcctcac catctgcgac gcgacgtccg cggcgttcgc
     9781 gatggatttg ccctggccga agctcgatgt cgttaccttc tcttggcaaa aggttctggg
     9841 cggcgaaggc gcccacggca tgctgattct ttcgccgcgc gccgtacagc ggcttgagac
     9901 ctacatcccg ccttggccca tgccgaaaac cttccgcatg accaaggacg gcaaactggt
     9961 cgaaggcatt ttcgaaggcg agaccatcaa cacgccctcc atgctggccg tcgaggatta
    10021 tctcgacacg ctgaaatggg cggaaagcat cggcggcctc tccggtttga tggcgcgcgc
    10081 ggaccgcaat ctcggcgtgc tcgcgaactg ggtgcagaaa accggctggg tggattttct
    10141 tgcccgtgat cccctcaccc gttccaacac cagcgtgtgt ctcaaggtcg ttgacccctg
    10201 gttcaccgcg ctgaatgccg aggcgcaaag tgcggctgcg tccagaatcg cgagcctgct
    10261 cgaaaaggaa ggcgtcgcgc tggatatcgg cggttatcgt gcggctcctc ccggcctgcg
    10321 catctggtgt ggggcgaccg ttgaatcctc cgatctggaa gccctcacgc cctggctcga
    10381 ttgggccttt gcccacgtca agaacggttg aaatcccatg cccaaagttc tgatttccga
    10441 caaattgtct ccggccgcga tcgccatctt caaggagcgc ggcgtcgagg ccgacgtgaa
    10501 gaccggcctc gccaaggacg aactcatcaa gatcatcggc gattatgacg gtctggccat
    10561 ccgctcggcc accaaagtca ccgccgatgt gatccgggca gcaaaaaagc tgaaggtcat
    10621 cgggcgcgct ggcatcggtg tcgacaatgt cgatattccc gctgcgaccg cggccggaat
    10681 gattgtgatg aacacgccgt tcggaaattc gatcaccacg gccgaacacg ccattgcgct
    10741 gatgatggcg cttgcccgcg acattcccgc agcgaatgcc tccacccatg ccgggaaatg
    10801 ggaaaagaac cgcttcatgg gtgtggagct gaccggcaag gttttcggcc tcgtcgggtg
    10861 cggcaatatc ggttccatcg tggccgatcg cgccaagggc ttgcggatgc gggtcatcgc
    10921 ctacgatccc tatctgtcgc ccgaacgggc aacggatctg ggcgtggaga aagtcgaact
    10981 caacgatctg atcgcacgcg ccgatttcat ttcgctgcat acgccgctga cgccggaaac
    11041 ccgcaacatc atctcggctg acgccattgc ccgcatgaaa aagggcgcgc gactgatcaa
    11101 ttgcgcgcgc ggcgggctgg tggacgaagc cgcagtgaaa gcagcactcg attccggaca
    11161 gctcgcgggg gctgccttcg atgtgttcga ggaagaaccc gcgacgagca atcttctgtt
    11221 cggcaatgag aaggtcgtgt ccacgccgca tctgggcgcc tccaccagcg aggctcagga
    11281 gaatgtggcc cttcaagtcg ccgagcagat ttccgattac ctcttgacgg gcgcggtgac
    11341 gaatgcgctg aacatgcctt cgatctccgc cgacgaagcg caaaaagtcg ggccttggat
    11401 cgcgcttgcg gaacatctcg gcgcctttgc cggacagctt acggaatcca gcatttccgc
    11461 cgtggagctt gtctttgaag gcacggcctc gacgctgaac acgcgcgcgc tcactcaggc
    11521 tgcgatctcg ggactgttga agcctgcgct ctccgaagtg aacatggtca atgcccccat
    11581 cgtcgcgaag gaacgcggag tgcgcatctc cgaagtgcgc cgcgatcagg aaggcgccta
    11641 tgagggctat atcaaactga cagtcacgac cgaccagcag gcacgcagcg tcgccggcac
    11701 cgtgttctcg aacaatcggc cgcgcctgat ccagatcaag aatatcgatc tcgatgccga
    11761 attcgcgccg catatgcttt acatcaccaa tgaggacaag ccgggattca tcggccgact
    11821 cggcatgctg ctcggcaatg cgcatgtgaa catcgcgaat ttcaatctcg gacgcgcctc
    11881 gcgcggagcc gatgcgattt gcgtcgtcca agtcgacgag cgggtgccgg acagcgttct
    11941 gcaggaaatc cggtcgctgc cctgggtgaa gcaggcacgc gcgctggcat tctgagtgcg
    12001 ttggttctga gtgtcttggt aaggaagagc gatgagtccc gctcttggtt tcattctggc
    12061 ttggacagcg tgggcccttt cctggggggc cgcggcggcc tggtcgtccc acaccgccaa
    12121 gcggtccgca atcgcatcgg cgcttgccta tcgcctgacc ataacagcgg gggccgttct
    12181 tcttttccac cgtacatccg agcggctgaa agcgccgatg ctttggcatg tcggactgat
    12241 tggcgcgtgg cttcttgtgg ctgtcgcggt gttgggtttt ttgttcacat ggtgggcccg
    12301 gcttcatctg ggcaaattgt ggtcgggttc ggtgaccctc aaggaagatc atcgcatcat
    12361 cgacagcgga ccctatggct tggtgcgcca tcccatctac acgggaatcc tggtggcgat
    12421 cttcgcgaca gccgcagccg aagcgaccct tccggcaatg accggagcgg cgctcatgag
    12481 cctcggcatc tggatgaaag cgcgcgtcga ggagaaattc ctgcgccaag aactcggcgc
    12541 cgcggcttat gattcctatg ccgcgcgtgt gccgatgctg ctgccatttt tgtgaggtcc
    12601 ttcgctcgac ccccggcagg ggacgcgcta tgacatcgca gcagattgcg gatgaagacg
    12661 gatggccaat gttgcggtga tcggcgccca atggggcgac gagggcaagg ggaagatcgt
    12721 cgactggctg tcggcgcgcg ccgatgtggt ggtgcgtttc cagggcggcc acaatgccgg
    12781 ccacacgctc gtcatcgatg gcgtgacgta taaattgtcg ctgctgcctt cgggcgtggt
    12841 gcgtcccggc aagctcgcca tcattggcaa tggcgtggtc gtcgacccat gggcgttcct
    12901 cgaggagatc gcccgcgtac aggcgctcgg cgtcgccgtc acaccggaca atctgatcgt
    12961 ggcggagaac gcggcactca tcctgccttt gcatcgcgag ctcgacgtcg cgcgcgaatc
    13021 cgacagcagc gttcaggcgc tcggcaccac caagcgcggt atcggcccgg cctatgaaga
    13081 caaggttggc cgccgcgcgt tgcgggtggt cgatctgaag gatttcgcga acctgccccg
    13141 caagatcgac cggctgctcg cccatcacaa catgctccgg cgcggcggca atctgcccga
    13201 aatcgatgcg cgcgccctgt tcgattcgtt gcgcgacatc gcgccgaaaa tcgtcccttt
    13261 cgcctcctgt gcgtggcggc gcctcgacga ggtgcgcaaa cagggtaagc gaatcctctt
    13321 cgaaggcgca caggccgtat tgctcgacat cgatcacggg acctatccct atgtcacctc
    13381 ctcgaacacg gtcgcgggac aggccgcggc aggttcggga atcgggccgc gccacatcgg
    13441 ctacgtgctg ggcatcgcca aggcctatac gacacgggtc ggcgaaggtc cgttcccaac
    13501 cgaactcacc gacgcgaccg gcgagcgtat cggcaaaatc ggcgctgagt tcggcacggt
    13561 cacggggcga aaacgccgct gcggctggat cgacacagtg ctggtgcgcc agaccgtgat
    13621 caccggcggc attgacggca ttgcgctgac caaactcgac gtgctcgacg gcttcgatga
    13681 gatcaagctg tgtatcgggt atcgtttgga tgggcgccag atcgattatc tgcccgcaga
    13741 cgcaaccgaa caggcgcgct gcgagcccgt gtacgagagc atggatggct ggaagggatc
    13801 gacccgcggc gcgcggagct gggtccaatt gccggcacaa gcggtcaaat atgtccgccg
    13861 cgtggaggaa ttgatcgggg cgccggtggc gctgctgtcc accagtcccg aacgcgagga
    13921 caccatcctg gtgcgcgatc cgttcatcga ccggtaaaca tgcttcacgc tgccggggaa
    13981 tccggcgttg gagcggcgct tgaatgccat tacccccccc ggcgattggc cgtctggcgg
    14041 ggaaaaacgc ccagtgtttg gcggaaaaaa caagaaacgc gcaagaaaat ttggtgcgaa
    14101 tagagtgcaa ttgggtgcca ttggcgcgct atggacttgc tcggtttgcc tcatcccgtg
    14161 agatatagga acccgatgtc attgcgtcca tcgcagcgac accattgaaa tccattccgg
    14221 ccggccatcg gctgcggatc atcttgcgtg aggattgacc gggccggtgc catgtccgaa
    14281 cgcgaggcca ttgcggatcg cttcctgcac gaaattccgt gcacggatga ccgcgtcgcg
    14341 aaggggcagg ccctgcgcca atcccacggc gatagccgag gccagggtgc atcccgttcc
    14401 atgagtcgag ggattggcga tgcgcggcgc ggtgaattgg tgtacaccct catcatccac
    14461 caacacgtcg gtgacaagat cgccggagat atgtccgccc ttcatcagga cggcacgcgc
    14521 accggcatcg cgcaggatca taccggcccg cgcgctgtcc tccggcgacc gcactgcgat
    14581 tcccgtcaac cgcgctgcct ccggtgcgtt aggcgtgatc aacgcggcaa gcggaaacaa
    14641 caatcgccgc atcgcatcca ctccggcatc gtcgagcagc gcgtggccac cctttgcgat
    14701 catcacggtg tcgaccacga gcggcacgga cgcataggcc gcgagcgcag ccgcgaccgc
    14761 ctcgacgcag gctgccgaca ccagcatgcc cgtcttgatc gcatccgcgc cgatgtctgc
    14821 aagcacgacg cgcatttgat cggcaacgat tcccggggga accgggtgaa agccgtgaac
    14881 accggtcgtg tcctgcaccg tgatcgccgt aacggcggtc atggcgtaac cgcccaacgc
    14941 ggtcacggtc ttgatgtcgg cctggatacc ggcgcctccg cccgaatccg aacccgcgac
    15001 gatcaggacg cgggcgggtt cgatcatgcg ctcgcttcgc gaatgacctg cgcgacctcg
    15061 cgcacgatct ggcgcaccag cttttcgtct tctccctcac ccatgatgcg gatgaccggt
    15121 tcggttccgg acttgcgcac gagaagacgg ccatggccgt tcaaccgcgc ttcgcaggcc
    15181 ttaatatgcg aggccactgt ggcatcgctc agcggctcac cgcgtttcag ccgcacattt
    15241 tccagcactt ggggcgcggg ttcgaaaata tgggcggcct cgctcgcggg ccggcgttcc
    15301 tccgccagca cggcaagcac ctgcaacgca ccgatcagtc cgtcgcccgt ggtgatgaag
    15361 tcgctgaaga tgatgtggcc cgattgttcg ccaccgacat tgaaaccacc ggcgcgcatc
    15421 ttctcgatca catagcggtc cccgacgggc gagcggacca gtttaagacc ctgggtggtg
    15481 agataacgtt cgagcccgat gttcgacatc accgtgccga ccacgccccc gcctttcagc
    15541 tcgccggact tcgcccagga acgcgcgatc agcgccagca cctggtcccc atcgatgatg
    15601 tagccctttt cgtcgcacat cacgacacgg tcggcatcgc catcgagcgc gataccgaaa
    15661 tccgcccgcg tctccttcac cttctcgatc atcgcggtgg gcgcggtcga tcccacctcc
    15721 tgattgatgt tcatgccatt gggatcgacg ccgatggcga acacatcggc gcccagttcc
    15781 cagagcacgg ccggcgcgac gcgataggcc gcgccatgcg cgcaatcgac cacgatgcga
    15841 agcccttcga gacgcaaatg cttgggaaag gtgcgtttga cgaattcgat gtaacgcgct
    15901 tgcgcgtctt cgatgcgccg cgcgcggccg atgttttcgg ggggggcgag gccgtcctcc
    15961 agcccgttct ccatcagcgc ttcgatttca tgctcgcgtt cgtcggaaag cttctggccg
    16021 ttggggccga acagcttgat gccgttgtcg tgataggcat tgtgcgaggc cgagatcatc
    16081 acaccaagat cggcgcggag cgatcgcgtc agcatggcga cggccggtgt cggcagggga
    16141 ccgaattgga acacgtccat cccgacggag gtgaatcccg acaccagcgc ggattcgatc
    16201 atgtaaccgg aaaggcgcgt atccttgccg atcaccaccc ggtgacgatg atccccccgc
    16261 gtgaagatgc ggcccgcggc catgccgact ttgagcgcga tttccgaggt catcggaaac
    16321 ttgttggcca aaccccggat gccgtcggta ccaaacatct tacgcgtcat gagacttccc
    16381 cctatcccgg ccgcaaagcg gaagggatgc tcggcattat aacagaacat aatccgcaac
    16441 aatattggcg ggcttcgatc atccgatgcc ggtcgcaata tgcgaccaaa tcgttaaggc
    16501 gtcgcgaaga gatttgggat cgtgcgtccg gattatgtcg gctccgtgaa gcgccgcgta
    16561 aagttcggcg gcaagcgacc ccggcccgat ttcctccacg ctacgccccg ccaattttcg
    16621 cagaaaggat ttgcgcgaca ccgaaacgag gatcggcaat ccgaaagcgg atttcagttg
    16681 ggaaagacgg cgcaggatcg tcagcgacac ctcaggcttt gtcccgacga acagccccat
    16741 gcccggatcg aggatcaaac gttcgcgcgc gatgcccgca tgggtgagcg catcgatcct
    16801 gtgctcaaaa aaatccgcga tccggccaac gatggtcctg ggatcggtat cgattctctg
    16861 ggcgcgcccc gtttcctgaa ccgcatgcat gacgacgagc ttggccctgg ctttagccaa
    16921 atgcgcatac agggatgcat cggcaaatcc ttgtatgtcg ttcaggtaat cgacttcttc
    16981 gtccagcgcc cagagttgcg tttcggatgc gaagctgtcg acggatattt tccatccccg
    17041 ctccttggcg gcgagaacaa tcggcgcgag ccggccgatc tcgatatcgg gcgacacggg
    17101 ttcggaacgc ggattgctgg aagctgcgcc caaatcgagc gcatcggctc cgtccgcgat
    17161 cagtttgacc gcgtgcgcaa gcgctgcctc aggatcgagg aaacgccccc cgtcggaaaa
    17221 cgaatccgcg gtaatgttca ggatgccgaa gatcgtcgtc atgtgaaaac gaccaagaca
    17281 gctacttctg gcttcacgtt cccggttgcg gctcaggcga gatggtgatg cccggttcgc
    17341 gcgcccggcc tgcttgcggc accgtcgcgg cggggcgtgg ctgggtctgg gactcgggtt
    17401 cctcatacgg cgtgcgcacc gggcgttcgc cccgcatcaa gcctttgagt tcgtcgcccg
    17461 tcagcgtttc gtattcgagc agccccttcg ccagcatatg caaatcctcg atgcggtcgg
    17521 acaggatttg cttcgcgcga ttgtaggtct cctcgatgat ccggcgcact tcggaatcga
    17581 tcttctgcgc ggtcgcctcg gacacgcttt gggtgcgcga gacagagtgg ccaagaaaga
    17641 cttcttcctg attctcgccg taggaaatcg ggccgagttc atcggacatg ccgaaacgcg
    17701 tcaccatggc gcgcgccatg cgtgtcgcca tttcgatgtc ggattgcgcg ccggtggtga
    17761 ccttgtcgta accgaagatc agttcttcgg cgattcgtcc cccgaaagcg acacacaaat
    17821 cggcatagag tttttggcgg gtcagcgaaa cctgatcgcg ttcgggcaaa cgcatgacca
    17881 tgcccagcgc gcggccgcgc ggtatgatcg tcgccttgtg gattggatcg gagccgacca
    17941 ctgtcagcgc cacaagtgca tgaccgccct cgtgataggc ggtgagcttc ttttcttcat
    18001 ccgtcatggc cagggtgcgc cgctccgcgc ccatcatcac cttgtccttc gcgtcttcga
    18061 attcggcgcg gctgacaagg cgcttgccgc gtcttgccgc aagcagggcc gcttcgttga
    18121 cgagattggc gagatccgcc cccgaaaagc ccggcgtgcc gcgtgcgatc acgcgggcgt
    18181 ccacatcggg cgacagagga accttgcgca tgtgcacttt caggattttt tcgcggcccg
    18241 aaagatcggg gttcggcacc acgatctgcc ggtcgaaacg gcccggacgc agcagcgcag
    18301 ggtcgagcac atcgggccgg ttggtggctg cgatgaggat cacgccctca ttggcttcga
    18361 aaccgtccat ctcgaccaac aattgattca gcgtctgctc gcgctcgtca ttgcccccgc
    18421 cgagaccggc gccgcgatga cgcccgacgg catcgatttc gtcgatgaac acgatgcagg
    18481 gcgcgttctt cttggcctgt tcgaacatgt cgcgcacgcg cgacgcgccg acgccgacaa
    18541 acatctcgac gaagtccgaa cccgaaatcg tgaagaaggg cacgttcgcc tcgcccgcga
    18601 tggcgcgtgc gagcaacgtc ttgccggtac ccggcgggcc caccaggagc acgcctttgg
    18661 gaatgcggcc gccgagcttc tggaattttt gcgggtcttt caggaagtcg acgatttcga
    18721 ccagatcttc cttggcctcg tccacaccgg ccacatcttc gaaggtgacc cggccctgac
    18781 gctcggtgag catcttggcc cggctcttgc cgaagcccat ggccttgccg ccacccgctt
    18841 gcatctgccg catcaagaag atcatcaccg ccacgaacag cagcatcggg aaccaattga
    18901 tgaacaccga cagcagcgtg atgttgtcgc cttcagggcg caccttgata tcgacattgt
    18961 gctcgagcag gcggcttgtc aggcctgggt cgtccgggat gtcggtagag aacggggttc
    19021 cgttcttgaa ctggccctcg acatgcgtac cggcgatgtt caccgtcttg atgccgtccg
    19081 cgacgatctg ctggttgaat tcgctgaaag aaatcggggt gcgtgcacct tgccggctgc
    19141 ccggaccctg gaacaggttg aacagcgcca ccagcagcag cgcaataatg atccagagtg
    19201 ccaaattgcg gaaattgttc acggccgacc tcttctccgg gcccgtcgtc tcaggggatt
    19261 aaacgccgac gcgcggtatt cgtctacaga taggtatttg tataccgact tgccagtcag
    19321 gaaacggtta agaattgcat cagaggggcc gaatacgacc tttatggggc ggatttcccg
    19381 gccgatccga aacggtatcg gcgttccgca tgagcttccc ggcgtttgcg ccgttcaacc
    19441 tcgaccggcg ggccggttca cgaacgatca gcagcgtcgc tcccccaaag acagcccgcc
    19501 ccttcggcgc tttggcgatg cggcaaccca gcagggtccg tgccgtgaac ggctccacag
    19561 aaattgcatc gaacagcgct tccaaccgct cgaatcgcgg ccggtatggc gcgcctgcaa
    19621 cacgcatcag gacagcactg agcacccgca agccgatttc acgggaattg tgcgcaagcg
    19681 cgccaccgtc cataagcgcg aaatcggcgc ccaattgcgt gtgacgttcg aagaattctt
    19741 cggttgccgc ctccaaagcc tcacgcgctc tggccagatg gcctgcggct tgggcaatac
    19801 gctgcaccga gactcccgcc gcctccagcc caggcaacgc ttggcggatg cgcacgcgcg
    19861 caaagcggca atccgcgttc atcgggtctt caagccaatg ttcgccccgt ccctgaagat
    19921 aggcgcgcaa ctcagtgcgg cccatgtcca gcaatggcct cagcaatcgc acaggcgcaa
    19981 agcccggcac gggcaaaggc cctgcggccc gcatgcctga caacccatcc acgccactgc
    20041 cgcggccaag gcgcaggaga aaggtctcgg cttggtcttc gcgggtatgg gcggcgaaga
    20101 ggtaaggcgt catccgcgcc ttgcaccatt cgccgagcag ccgataccgc gcggcgcgcg
    20161 cctcatcctc gatattcgac tggggcttgc ggcctcgcca acgcatgacc gccgcggcga
    20221 agcccgcgtt ctttgcccat tgtgcaacca gggcggcttc atcgtacgat tcgcgccgca
    20281 aaccgtgatc gacgatcaga accgcgggtc gcgaaatgga ttcgcgcctg gcccaatcgg
    20341 cgaaaagatg catcagcgca atcgaatcgc cgccgccaga aaccgccagc gccgcggcga
    20401 agccgggcaa aaagccaagc tccgtcagcg cacgcgaaaa gcgctccggc agatcgcgcg
    20461 cgtgtctatt tacacttgtt ggtggcgcgt tcggacttgg ctctggtcgc gacggccggc
    20521 gaggcatcgg gatattttgc gggcagggcg gcaatcgcag cacaaccttc cttcatctgc
    20581 ccaagtccga aaagcgcaag accgagcttc agcatgcctt cgggcgcgcg ctgggacttg
    20641 ggatatttct tcagcagctc cgcgaaatcc cgggccgctt ccggatagtc cttccgggca
    20701 gaataggcga tgtcgcccgt ccaatacagc gcgtccggag cccggtcctc atcggggtgc
    20761 gcatccgcga atttgcggaa cgccgcactg gcgggttcat attgcgcgcg cgatagcaga
    20821 ttcatcgccg tatcgaattc ggccttggga ctggccgatg gcgcgggcgc agcgggaatt
    20881 gccgtgccgg ccggaatctg cccgagattg gacgggggcg cgcctctgag tggcgagaga
    20941 ttctgatccg gtgtcagcga agccaccgca ttgccgctgc ccgccggcaa aggcggcagc
    21001 ccggccgtag ccgaaggcgt atcggtattg gccaccggcg cagtttgctg ggcggcctga
    21061 tattccaatt gtttttgcaa gcgatcggtc ttctgcgaaa gctcatcgac ctgatggccg
    21121 atttcttcca tctgacctgt cagccggccg aggccgtcct ccaggtctcc caggcgccgc
    21181 atcagcggat tcaaatccgg cacagggggt tgttgagtct gcgcatcggg aataggagcc
    21241 gggatgagtc cggggctcgc ctcgacaggg gccggcgcgg gccccgtacc gccggagcgg
    21301 cggaaggttt cagcctggac gtcgtgcatg tcgcgctcca gacgctgaag ccgcgccatc
    21361 agatcgcgcg catcaatgac ttgggttgcg ggcgcagcag acgcgccact cacccccgcg
    21421 ccactcaccc agacgaatcc tccgaccagc gccgctcctg ctttcatcca tcgcccgcgc
    21481 atcgctttca tcggctcgcc tcttgctgga ggaaacgaag attcgccaat tctatcctga
    21541 aacaaccgat tctatgccga aaaatggtgg aaatagggct gtaagaatgt ggaaaaaaaa
    21601 cagggaccgg aaattttatt tccgatccct gccgattttc gcttgagggt ttgcgattac
    21661 gaaacgctac cgctcttgat cgtcgagacg ccccgccggt tttgcgccca gcacgcttcc
    21721 gtcgattcga cgcaaaccgg gcgttccttg ccgtaggaga tcgtatcgag gcgtgcggcc
    21781 gatacgccca ggcttgcgag gtaatccttc tcggcctgcg cgcggcgcgc accaagccca
    21841 agattgtatt cgcgcgtgcc gcgttcgtcg caatgacctt ccaaggtcaa ggtcacggca
    21901 ccgaagcgtt gcagccatgc ggcttgcttc tgcagcgtcg cgcggccggc ttcggtaaga
    21961 tcggttttgt catattcgaa gaaaacccga tccccgacat tcacctgaaa atcctgcacg
    22021 ctgccgggca cgatagattg caccacgggc gcaggtggac gcggcggcgg tgccggcggc
    22081 ggtggcggcg gcgcgggcgt cgcggcgggc ggtggcggag gcgccggagg aggtgcttcc
    22141 atcattttgt ccgcgcatgc cgtcagcatc agtacgaaag aaaggctcgc caactttgcg
    22201 ctcgtcgtga gtttcgtcat tttttatgga cctccgttat gcgcagggcg ctctaggtgc
    22261 ctaggcgatg cgcttctcag ggaaatgtcc cccttaaata tgcgactcgg acaaaatcgt
    22321 ttccgaatgg aacaaaatta cgggaagcca ttcactgtat caagggcgac catgcgggat
    22381 ccgacgcatc gccaggagaa agcacgcgcc gctcgttgcg gccggtgatg tcgaccgacc
    22441 agatttgcga tcccccaccc gcaccgccgg ctccggtttg taacgtacgc gtgaacatga
    22501 tcacccgccc attgggcgcc caggtcgggc cttcgtcctg ccagccatgc gtcaaaagcc
    22561 gcgtccccga cccgtcgggc cgcatcacgc cgaccccgaa atagctcggg gtcatgttgg
    22621 taaaggcgat caggtcgccg cgcgggctcc atacgggcgt gccattcttg ccctgaccga
    22681 aactgattcg atgcacgttg gttccgtccg cattcatgac gtagatctgc tgcgaaccgc
    22741 cgcgattgga ctcgaaggtg atctgtttgc cgtccgggga gaacgagggc gcggtgtcga
    22801 tcgcaggatc gttggtgagg cgcctggatg tccgcgtcgc cagatccatg acatagattt
    22861 cagaattgcc attggtctcc agcgccatga caacgctctt accgtccggc gaaaagcgtg
    22921 gagaaaaggt catattgggg aaattcccca acatttcctg ccgcccggtt tccaaatcga
    22981 agagataaac ccgcggacgg ctctggatat aggacatata ggcgatcatc tgcgcggtcg
    23041 ggttgaagcg cggagtcaga accaaataat cgccctgcgt gaggaacacc gggttggcgc
    23101 cgtcctgatc catcaccgcc aggcgtttgc ggcgattgag cttgggaccg gattcggcga
    23161 cgaaaacgat gcgggtgtcg aaatatccct tttcgccggt gatgcgctca tagatcgcgt
    23221 ccgagacgag gtgcgcgatg cgccgccaat tctctggcgt ggtcataaac tgcaacccca
    23281 ccatctggct ttcgccgaag atgtcccaga gacggaaatc gaccttgagg cggccatccg
    23341 gctgcatcac ggcctgaccc gtcaccaggc cctgcgcctg gatggtgcgc caatccccga
    23401 accgcggcgg cgtattgata tctgtgatct tttcgatgaa ggaggcgggg ttgagcgagc
    23461 ggaagagacc ggagcgctcc aaatccccgc gcaccacgcc ggcgatgtcc cgccccgcct
    23521 ggccagtgcc cagaaaatcg ggaatggcga gcgggatcgg ctccgtgaca ccttgatcga
    23581 cgcgcacctg caacgcggcg ttcgcgggtt cgaacacgcc cgcaaccatg cccaaaccaa
    23641 gcacagcggc gagaatgaaa tgccgcgaga ggtgtctgaa agagttttgc gcgtgcatgc
    23701 ccatggtcaa tcctgattcc tgacggcctg tatcctaatg gccggtcatc ttgctggggt
    23761 cgaatgtcat cacgatttcc cgccaggtat cgtatttgtc cggcggcaga cgcttatacg
    23821 gggcgcaaac attgatcgca cgcgatgccg cctcggccgc ggtacgcata tattgattcg
    23881 tagcagcggc ggcacgggtg gcgggctcca actgcggagg ctgcaaaagg cggccatccg
    23941 gcgcgagaaa aacgcgcacc tgtacgatga gttgctcggg attgggcgcg ccggccggca
    24001 cgttccagca ttcgcgcatc tgggcgagaa gcgcatcctt gatgtccatg gtcagggcgt
    24061 tttggtcacc cagacccttg accgtctgct ccgcgagctt ggcattcgcg ggcggtgctt
    24121 caggcttggg cgcgcgcttg tccagcagcg cgatgaccga atcgacatcg aatttcgcct
    24181 gcggcgttgc ttgcggcttg cgccggggca gggccggttt ggcgggggcc ggcgcagcct
    24241 gctcggcttc tttaggcaat tcctttggct tgggcgtcgg ggttttgtcg agatcgggag
    24301 gggcgacttc agcctccggc ggcggtggtg cggcgacctc cggctgcggc ggcgcgggtg
    24361 tatcgggcgg cgtctcgacc ttctttgttt cctgcacggt gggcgcgata ttggtcacgt
    24421 cggcaatcgt caccagatcg accggaacca cgggcggaga ttcatccgag aggttgagcg
    24481 catgcggcca agcgacggtc gcggctacga tcaccgccag atgcagtgtg cccgagacca
    24541 caatgcttcg cttcagcatg cccttcggtt catcctcact gacgcctcac ttattgacgt
    24601 ctcatgctcg ctggagaggg cgtctcgata ggcgagccgc cgcgcgccgg catcggctat
    24661 tttaaaggac gttgtggcgg ctttttttgg gcatcggtga caagaccgat ccgggtgaag
    24721 cctgccgaat tcaaaagtcc catcacctcc atgaccttgc cgtaatccac ggctttgtcg
    24781 ccccggacga aaatccgctg atcgtagcca ttattggaga tcgccatgag cttcggcacc
    24841 agatcgtcga tgccgatcgg cgtgttctgc aaaaatatct tgccgccatt atcgatgctc
    24901 acggcgagcg gctcgcggtc ctcgcccaag gacttcgccc gtgtctgcgg caaatccacg
    24961 ggcacgccca ccgtcagcag cggcgccgtc accatgaaga cgatgagcag gacaagcatg
    25021 acgtccacaa acggtgtgac gttgatttcc gacatcggac gcccaccgcg catgcggccg
    25081 ccgccgcgac ggccgcgtcc cgctcgcgga ttgttcatca ctgcgggcat cagcgcgcct
    25141 tctcgtcgag ctggcgtgaa aggaaggtgg agaattcatc ggcgaagatc tccagccgtc
    25201 ccgtataacg gccgagctcg ttgacgaagc gattataggc gataaccgcg gggatggccg
    25261 cgaccagccc caaagccgtt gcgaacaacg catcggcgat gccgggcgcc accaccgcaa
    25321 gcgtcgtgtc gttgcgcgcg gcgattgccg tgaacgccgt catgatgccc cagaccgtac
    25381 cgaacaatcc gacgaagggt gcggtggaac cgacggtggc gagatagccc agaccgcgtt
    25441 cgatcttttc caactcgcgc tggatcgaaa tattcatcgc cttttcgacg cgatccttga
    25501 cattggcgag ctgggtctcg ggtggcgtcc cgccttcgaa agaacgcttc cattcgcgca
    25561 gcgccgcaac gaagaccgct gtgaacggtt ggtcggtgcg ctgctgatgc tgcgcgtaaa
    25621 gctcctccag cgagcggccg gaccagaagg acttctcgaa acgtttggct ttgcgcttca
    25681 acgtggcgaa ggcgatcaat ttatccacga tcagcaccca cgaccagaac gatgccagca
    25741 acagcagaac gagcacgcct ttcacaacga tatcggcacg taggaacaaa ccgaccggcg
    25801 tgaaggcttc ctggatctct gcctgcgagc cggcaacatt cgcatctgcg atcccgcttg
    25861 gcgcggcaag cggggcggac tccgcgggcg ttccggaagg tgccgcgctg ggggggcgtc
    25921 ctccggaggg tgctgcccgc tggaactgcg caaacgcgga atgattcgcc ccgtccaaag
    25981 tgacacacaa tagaagcgcc atgagagtga tggcggctaa acgcaaaatt gtcatcgccg
    26041 gataccggag caaattttcg agcctggtct tgggagcgct tgcggtaaga cttgcgccat
    26101 tgcccttaag ggcgggatga aaaacgaacg tccagtgaac tcattcaaac atggctgaat
    26161 tttgaccctc attcctgcat ccctatgtcc tgcatcccta tgtgaagcgc ggtccagcgt
    26221 gcccggtttg cggatgtccg attctcggat gaacaaaagg acatcactcg gaaagcccga
    26281 gcgccttgcg aagttccgcc ttggccgctg caaagtcagt ccgaaaatcc gtgttctgca
    26341 tcatgagcgc gaccatggcg gtggcttcga tcctgcctga ttcgacgtcg gtcggaaaat
    26401 gaactccgcc gatgattcgg ctctggccat aatcccagcc ccgcacaaac agttcgttgc
    26461 gtttttccgg caccatgttg gcaagcagga tcgcggtcat tgcgccgaag gtcgaatggc
    26521 cgctcggata ggaataggag aattccggcg agggcgattc gaccggcagg caaggcgatg
    26581 ccctatcgtg gggcgcgctg tttgcgggcg tcgcgcccgg ccggttggcg gtttcctctc
    26641 gcatcgcgcc tggcggatga acccggtcat cggccacgaa cggccggggc cgctcccagc
    26701 aatccttggc catgttgacc ggctcccgcg tgtcgcgtgc caccttgcgg aagaacgcgg
    26761 cgacaccggg aacagcggtg aaagagaatt ttgtgccgag cacggcggcg aactgcgcga
    26821 tgtcgacctt cgcgtcttcc tcggcgcgtt ttatacgctc cggcgaggcc gtctgctggg
    26881 cgcgcaggac gccatcgata tcctgccctt gctgcggcga agtcgcgcgt ggcggcggcg
    26941 gcagcagatg aacgctgtcg aacgcaccgg gcgcgagata ggacgtatcg gacagagaaa
    27001 gtgccggcgt tgcgcccaga agaaggagca acgccggaac gaagaaacat ttgcgcatgg
    27061 aaaatccttc cgatgcgcgt cttatatcag cttgagccaa ggatatcagt ttaagctcag
    27121 tatatcagtt taagctcagg cccttgcgga tctccgcttt gaccgcctca aaatcggtgc
    27181 ggaattccgg attcttcagc atttgattca ccgcgagcga ggcggtgatg tgaccacttt
    27241 cccagtcgga cggataggcg gcgccgctgg ccatgcgatt gacgccatac ccccagccgc
    27301 gcgcgtacag cgcttcacgc ttttccggaa ccatcatgct catgagaatc gcgacttcgg
    27361 cgccggtcgt gctgtgaccg ctcggatagg tcgggccacc cgacaccttg gcgatatttt
    27421 cgggtgtctt gacgtccttg ctgtactgga atggacgcag ctttttgtat ttctgtttga
    27481 tcgggccgat tccgataccg gcgtctttcg tgactttggc gaaaaattcc cctgccagcg
    27541 gaaaattttc cttggtgaat ttcggaccca ggaccggagc ggcaaccaca tagacgtttt
    27601 gctccagatc cctctggatg ttcgccatcc gctccggtgt catattcttc tgaagatcga
    27661 gaatggcctg caagtcacgt ttgccggccc ccgaatcctc cttcggcggc ggtggcaaca
    27721 gtgccgggaa gtcgacatcg gttgctgcga tgtagggttt gtcggacaag gccagcacgg
    27781 ggctggcggc gagcgacaaa aacaatagcg acaaaaacaa tattggggcc aaagcgagct
    27841 tgcgcatgca ttcctccgtt gcgtgtgagt ctattatgga cttttgagga aaatttccaa
    27901 tttttttcgt gtggagtcgg gcacgcggcg tggccgtccg tcaagggtga tgacgcagac
    27961 ctccacatcg gccttcacca atgcgagccc tgcgcgcatg acgctttgag caagccgcat
    28021 gcgcgcacct gccaatgcga gaacctcggt gtgcacgctc agcgcgtcgt ccatgcgggc
    28081 cggcttgacg aaatccgtcg ccatgcgcct gaccgcccac accagcggtt cggcgttctc
    28141 cagcatgccc tgatgccccg ccccggcaag acgcaggaat tcggagcggc cccgctccat
    28201 gaaacgcaga taattcgcat gatagacgac accggagaaa tccgtgtctt cgtaataaac
    28261 ccgcaccggg aagacgtgga tattcccttc aagccagccg cttgaaagtc cgggtggaga
    28321 ggttgagggg tcaggattcg tcatcgccga acaattgcgg ctggcccggt ggcgtactcg
    28381 gtcccggcag cccgagatgc acataggcct gaccggtcag cacgcgcccg cgcggcgtgc
    28441 gctgcaccag gctctgctgg atcaggtagg gctccacgat ctcctcgatg gcatcgcgcg
    28501 gttcgccgag tgcggccgcc agcgtctcga tgcccacggg accaccgttg aagccttcgg
    28561 cgatcagcgc cagatagcgc cggtcgaaag catccagacc ccgtttgtcc acttccaatc
    28621 tggcaagcgc cgcatcggcc accatctgat cgacctcgac gcgccccgca acggcggcga
    28681 aatcgcgcac gcgcttgaga aggcgcccgg caatgcgcgg cgtaccgcgc gaacgcatgg
    28741 cgatctcgcg ggcgccatct tgggtgaggt tgagacccag cacgcgcgcg ccgcgcgcca
    28801 cgatcagcat caactcgttg gtctcgtaaa aggcgagacg gatgggaatg ccgaagcggt
    28861 cgcgcaacgg cgtggtgaga agtcccgagc gcgtagtcgc cccgatcagg gtgaagggcg
    28921 ccagcccgat cttcaccgaa cgcgccgagg gtccttcgcc gatgataagg tcgagttcga
    28981 aatcttccat cgcggggtag aggatttcct cgaccgccgg attgagacga tggatctcgt
    29041 cgatgaaaag gacatcgcgc ggttcgagat tggtgaggag cgcggccaga tcgcccgcgc
    29101 gcgcgagcac cggtccggat gtcgcgcgga accccacgcc cagctcacgg gcgacgatct
    29161 gcgcgagcgt cgtcttgccg aggcccggcg gcccggagaa cagcacgtgg tccaatggct
    29221 cgccgcgggc cttggcggcc tcgatgaaga cggaaagatt ctcgcgggcc tgtttctggc
    29281 cggtgaattc tgcaagccgc ttaggccgga gcgtggattc aagctcgtcg tcgtcactgc
    29341 gctcgggcgc cacgacgcgg gttccatctt ttgcggggtg ctgtttggat ttgggcgacg
    29401 ccattcgagt ctcaccgcgc caattgccgc aaggattcgc gcaacagcac atccagcggc
    29461 aacgcctccg cattgggtcc aatgttctgg cttgcccgcg cgatggcctc ggctgcggcc
    29521 atttgcgaat agccgagttt catcagcgcc gcgatggcgt cggactccac cgggtgcggc
    29581 gcctgcagtc cacccacgcg ttcgccggtt tccgcactgc gttgcagcgc gagcgccggc
    29641 gccttgtcct taagctccgt gacaatgcgt gtcgccagtt tcggacccac accctgggca
    29701 cgccccacca cgctcttgtc gcctagcacg atagcgcgtt ccaattcggc tattcgcagc
    29761 gccgacaaaa tcgacagcgc cacgcgtgcg ccgacgcctt gcacggtttg cagcaggcgg
    29821 aaccattccc gctcgtcccc ggcaagaaac ccgtacaacc gaatcgcctc gtccgacatt
    29881 ttcgtttcga cccagagcgt cgcgctggcg cccgcgctca agcgcgacag cgtcgaagag
    29941 gcgcactgca ccacatagcc gacgccgccg acatcgatga tggcatgatc cgtgcccaga
    30001 aaatccaccc ggccggtgag cttgccgatc attgcgccaa ttgccgttcc agggccgcgg
    30061 cgatgcgcgc gcgcgtcccg gcgagatggg catgggcaat cgcgcaggcc agcgcgtcgg
    30121 cggcgtcggc ggtttcgaat tgcaccgcgg gcaacagccg gcgcaccatc gcctggacct
    30181 gcgtcttatc ggcatggccg acgccgacga cggatttctt gatgtggttg gccgcatatt
    30241 cgccgatcgt cagccccgcc tcggccgccg ccagaagtgc gatcgcccgc gcatgtccga
    30301 gcttcagcgc cgccagcggg tccttggcca cgaaggcctg ctcgaccgcc accgcgtgcg
    30361 gcgtgtgcgc ggccagcacc gcacggatcc ccgcatgcag catggccaga cggacgccca
    30421 gcggatgggc ggtcgtcgtc acgatgacgc cgttggcgac atgggtcagc ttcgcgcccg
    30481 cgacttggat cacgccccag ccggtgcgcg aaagccccgg atcgaatccg gcaatgcgga
    30541 tgctggtcgt caacgcgggc tatcctggag agaatcgctt tgctcagtgt cgttcgagag
    30601 cgcgccggct gcaatgtccg aagcgtgtcc catcatctct cggcgtggac gcgaaagccg
    30661 aacgccaaga cgggaccgcg ctcggcgttg taggcgggat gagcgacata ttgccaatcg
    30721 aagctcagtg cgagcccctc gcgcaccgcc agacgataat aggtttcgac gatgtcttcc
    30781 gagccgtaat gcgccagcct gccgtcaccg atcagaatcc caagaccgcc ggcagcgaaa
    30841 tagtcttggg ctggctgcga aagaccgttt acgacaccgg cgaggcccaa cgcgtcgtcc
    30901 gggcggcccc aggccgttcc acggagcgaa agaccgcctg agagcgattt gttgatctcg
    30961 gtgaactcat aggcctcctt agtgccatca ttcgcgcttg cgcgcaggaa catgccccag
    31021 cccggcccga gttcctgttc gatattgagc gcaaatcccg gacgtgtcgc ataatcgcga
    31081 acggcggcga catccggggt ggcgccgctt gcccgcgccg tctcaacggc cttctcatag
    31141 tccgccatgc ggccgcgatt ggcgaacgcc agcaacttga tcttgccggg gtgcgcaccc
    31201 gcagtgaagc gggcttccaa ttccgaagtc acttcgaact ggccgaatcc gcgctgcaga
    31261 aaccggctgt tcggtacgcg cgagaggtcg aaagccccaa tccgcaaggt ccaccaatcc
    31321 tgcgtccatt cggcggcccc gccataggtg taaccccacg catcggccgc atagtcgaac
    31381 gccccggcat cgatcaccga ccagttcaaa aaatcgctgc gcggatcgtg cgcgtacatg
    31441 ttggtgtcga aaatgtccgt caccgcaaat ttgcccagcg taacgatcac attgtccgac
    31501 aggcgcgaac caccgagctg attggccgcc ggcgcaatcg aaacgtcctc gccgctcaga
    31561 tcgaaggttt gccgggcgaa caggcgtgga atgcgcaaat agggcgagga atcgccgatt
    31621 ttataagcct caccactgag atacccggca acgcccagcg tgttgctcag accgaaaccc
    31681 tgatcggcct cgatgtcgat ataggcttcg gtgtgctgcc acagccgtgc tccggcaaag
    31741 agcgttgcgt caaaggtctc gttgccgcgc gatcccgggt cgaggctatt cgcgccgcga
    31801 tagggcgaac ggaaggccgg atgatattgc agaacgaaag tcgtttggcc gtggaggctg
    31861 taggtttccg gttccgcgtc ggccaggacc ggtgaggatc cgaacacaca cagcaccagg
    31921 aaaagaacga aaagccgcat cagggaacgc atggctccgt cctatcccaa acggcacaac
    31981 actgcgcgat ctctgcaaag cttttatgca gcagagagct tctcgatcag cgcttcgctc
    32041 atttcgtaat tggcgaagac gttctgcacg tcgtcatgat cctcgagcac gtccagcaat
    32101 ttgctcagcg tctcgccggc ctcatcgctc accgggacgg tatttcccgg acgccagatg
    32161 atcttggcgc tttgcgcagg cgcgccaagc ttggcttcca gcgcgtcccg cacggcgga
//
LOCUS       DASYEG010000110        14662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4816187, whole genome shotgun sequence.
ACCESSION   DASYEG010000110 DASYEG010000000
VERSION     DASYEG010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 14662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14662
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4816187"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /gene="tsaB"
                     /locus_tag="VGM72_04390"
     CDS             <1..571
                     /gene="tsaB"
                     /locus_tag="VGM72_04390"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY4264532.1"
                     /translation="QSLMRSSGLDFTALDLIAVTTGPGTFTGQRVGLAFARALRVALN
                     KPAIGVTTLDCLAEEALVRQPRPWAIAAADAKREEIYLGARSARGETLIAPELIARDM
                     VAARLSALANLHGNRVALAGTAAEAVAPLLAEIGWQCTDSLVRQPDAIFVAGLVAAEA
                     RAGVHDPIPPPPKPLYLRAPDAKLPGRAL"
     gene            568..1062
                     /locus_tag="VGM72_04395"
     CDS             568..1062
                     /locus_tag="VGM72_04395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4264533.1"
                     /translation="MTAIIRGAMPGDAPLLARLHGASFDEPWDADSFRRLMERPGALA
                     FLGANEAATDLQAFILVQVAADESEILSIATLPAARRLGLARALIMQAAAEASKNTAI
                     AMFLEVAEDNFAALALYRDCGFTAQGRRRAYYVRRGGSSADALTLRARLPLKQVMGMM
                     RGLD"
     gene            1093..1542
                     /locus_tag="VGM72_04400"
     CDS             1093..1542
                     /locus_tag="VGM72_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEY4264534.1"
                     /translation="MNRTEENLNRIEKACNEKGLRMTEQRRVIARVVAEAYDHPDAEE
                     LYRRAISVDPHISIATVYRTVKLFEDAGILERHDFRDGRSRYEEISEHHDHLIDISTG
                     RVIEFVNEDIEKLQRRVAEELGFELVDHRLELYGIPKGGKKPGPPTQ"
     gene            1575..2447
                     /locus_tag="VGM72_04405"
     CDS             1575..2447
                     /locus_tag="VGM72_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEY4264535.1"
                     /translation="MASLRAFCILFVFATATLAGIPLQWLAVKLGLNAARTFPRLYHR
                     FVCRLFGVRVAVTGVPLRGGGLMAANHTGWLDIPILSSIAPVSFVAKSEVGRWPFFGT
                     LARLQRTIFVRREKSKAAEDRDNIKMRLIEGDTLVIFPEGTSSDGNHVLAFRSALLSA
                     AELGLGEDAGHHRLQVPVQPVSVAYIGVHGMPMGREIRPFFAWYGDMELVPHLWEALK
                     TGPIEVAVEFHPPLTIGQAGGRKALAARAEAAVRSGLIRALAGSNAAVAPPQQDEALL
                     EALSEAEDENADAA"
     gene            2526..3881
                     /gene="miaB"
                     /locus_tag="VGM72_04410"
     CDS             2526..3881
                     /gene="miaB"
                     /locus_tag="VGM72_04410"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEY4264536.1"
                     /translation="MKTLYIKTYGCQMNVYDSARMADLLAPLGYMPADRPDGADMVIL
                     NTCHIREKATEKVYSELGKLKQMKTLRAQSGNPMLVAVAGCVAQAEGAEIMARQPAVD
                     MVVGPQSYHRLPEMIARIARGAGHAMETDFPAEDKFDSLPEEQAVSGPAAFLTIQEGC
                     DKFCTFCVVPYTRGAEFSRPAMQIEAEARRLADKGVREITLLGQNVNAYRGAGPDGSV
                     WNLADLIRHLARIKGVARLRYTTSHPRDMDVSLIEAHRDVEALMPYLHLPVQSGSDRI
                     LKAMNRGHTIAEYLRIVERIRVARPDIALSSDFIVGFPGETDADFESTMSLARMVGYA
                     GAFTFKYSARPGTPAAATPKQISDAVRDERLRILQELIAAQQAAFNRSCVGRVFPVLF
                     EKPGKKPGQAVGRSPYLQPVHVEGAHALMGEIRDVRVAQTLPNSLKGVLANAQHPVPS
                     H"
     gene            3878..4903
                     /locus_tag="VGM72_04415"
     CDS             3878..4903
                     /locus_tag="VGM72_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY4264537.1"
                     /translation="MNRRHGTNAPDAAAHEQVTLEFTRNALMPALTGNHSRNLVRLEQ
                     KLGVKLAQRGNLIAVEGAPEARAHAATILRALYERLEAGEELTMGDIDAEIGFAAGPS
                     HELPQRDRAGTLRTAAGKLTRPRSAAQGAYIELMRNRELVFGLGPAGTGKTYLAAAFG
                     AHLLHERRVERLILSRPALEAGERLGFLPGDLKEKIDPYLRPLYDALFEVLGERCTKL
                     METGIIEVAPLAFMRGRTLSRAFVILDEAQNCTPAQMKMFLTRLGEESRMVVTGDPSQ
                     SDLPNGPEGLDSAVRYLDGVEGVGIVRFTAADVVRHPLVTRIVAAYDAHEPDTKARSR
                     QVRRDLE"
     gene            4900..5406
                     /gene="ybeY"
                     /locus_tag="VGM72_04420"
     CDS             4900..5406
                     /gene="ybeY"
                     /locus_tag="VGM72_04420"
                     /inference="COORDINATES: protein motif:HMM:NF014216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY4264538.1"
                     /translation="MIGRAAARAGQPAIVFLIEEARWREDAAVLRVIRRAVRLGLPKK
                     KTGESVTILLTGNAKLRALNAAFRARDVPTNVLSFPAAKGDPSSFGDIAMAYGVVRRE
                     ARAQKKDFAAHAAHIALHGTLHLLGYDHEESKDAEIMESLEVRLLATLGIADPYRPRP
                     YTKGRKKA"
     gene            5416..6396
                     /locus_tag="VGM72_04425"
     CDS             5416..6396
                     /locus_tag="VGM72_04425"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY4264539.1"
                     /translation="MPADSDTKSGPVAVLASPPQTETADGRPAGLPARALLSEIATRL
                     AQAVRGLRRDSAMRESLEEMIEESEREAKELTSQERMMVTNLLTFGELSVSDVMVPRA
                     DIVAVEEQIAFADLVALFREAQHSRLPIYRETLDDPLGMLHIKDVFALIETGEDGQLR
                     WTPTPITKLKREVLFVPAAMPALDLLLKMQATRIHLALVVDEYGGTDGLVSIEDLVEE
                     IVGDIDDEHDVEEVPDIVARPDGKYDADARVDLDDFKERTGIDLTGMQTEEDVDTLAG
                     IVAAALGRVPVRGEIVSHGGVEFEVLEADPRRAKRLRIRALPVTAPVAAG"
     gene            6399..7967
                     /gene="lnt"
                     /locus_tag="VGM72_04430"
     CDS             6399..7967
                     /gene="lnt"
                     /locus_tag="VGM72_04430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY4264540.1"
                     /translation="MRLSTAFLDRTAASLQRVRGFKRVAAAAGLGAMGALGFEPFRAF
                     PLLLLSYAGLVLLLDGASASPRRLRHAFGIGWAYGFGFFLVGLHWIGYAFLVDAAAHG
                     WEIPFVAILFPGGLGLFFALGAAGCMLRWRPGVQRIFLFALVFAIVEWLRGHVLTGFP
                     WNLPAYGWGASLGVLQSASVFGAYGLSLLTLLFGASLAILPGASAQARWLPVTITLFF
                     LALWADGELRLFTATDAVVPGVHLRLVQPDTPQSEKYVQRYMERNWWRLIDLSAAPAP
                     IAPTHIIWPEAAPPFVLARAPGALSEIARLTGDSRVLLTGEVRIAGEGPAARFYNSFA
                     MFGPHGKLLATSDKFHLVPFGEYLPFESALRSIGLTEIAANTGFSSGPGPRTFSVPGA
                     PPMGPLICYEVIFPQAVTGDPRPSWLVNMTDDSWFGPNAGPMQHFLIARVRAIEEGLP
                     IARAANSGISAVIDGYGRVQARLDLGLRGHLDSDLPIALPATPFARYGNFIMLALYVI
                     CAGAALWPPSLSKA"
     gene            8037..8450
                     /locus_tag="VGM72_04435"
     CDS             8037..8450
                     /locus_tag="VGM72_04435"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY4264541.1"
                     /translation="MPKKQANPVDGHVGHRVRLRRMLIGMSQERLGDLLGLTFQQVQK
                     YEKGINRIGAGRLFEVAGILGVPISFFYEDAHAGAASVSGFAEGDEPPPVMEFLSSGE
                     GLQLSLAFMRIKDGKVRRRILDLVRSLANEDGDIN"
     gene            complement(8478..8798)
                     /locus_tag="VGM72_04440"
     CDS             complement(8478..8798)
                     /locus_tag="VGM72_04440"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEY4264542.1"
                     /translation="MTTHPIDRLFQRVLARKGEDPNLSYTAKLLKAGRLKCAKKLGEE
                     AIETSLAAVAEDKTAVANESADLLYHLMVLWAACEISPDEVYDALAQREAQSGIDEKA
                     SRKS"
     gene            complement(8839..10599)
                     /locus_tag="VGM72_04445"
     CDS             complement(8839..10599)
                     /locus_tag="VGM72_04445"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY4264543.1"
                     /translation="MNNFKLVIAAGFACFFAAGTVHADAGDDLSRLMRDYRVSEGKLR
                     PDESSGVYDEKRNADTLRARQRINENTRGKLASIDALILKAQDLLSYEIFGWSLDDEA
                     RELSPAIAENFQLLALNQFDGAQIIFPRQMQSRSEHPWTRSKDYDDAIGRMLRFTNGI
                     DAAIGKMREGIKRGIVQPRAIVTRMIAQTEIFANGDPDDGLFMAPLKTMPDPIAGAER
                     ARIEGAYREAVEGQLAPAYRRLLDFLKNEYLPHARESIGLSAIPGGKETYLYLVRSET
                     TSDLTPDAIHALGLSDLARIEAAMERVKNEAGFSGSLADFRVFLHNDSRFKFKDKDAM
                     LGEFTRVKNTVDAHLSQLFITKPKSDLVFRFYEPYVAPDRPAAEYTSASADGRRPGIV
                     SLNASDLGARPAYTSEALAIHEGIPGHHMQVAISMENNALPRFRRFGYETAFTEGWAL
                     YAETLGSDLGLYTDPYQKFGALSFEAWRSSRLVVDTGIHWLGWTRFQAIAFLQTHAGL
                     SENEASEEVDRYIAIPAQALSYRIGEREILDLRNRAKAALGPKFDIRLFHEAVLKDGA
                     MPLPILDAKINRWIASQKGT"
     gene            complement(10680..11546)
                     /gene="gluQRS"
                     /locus_tag="VGM72_04450"
     CDS             complement(10680..11546)
                     /gene="gluQRS"
                     /locus_tag="VGM72_04450"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEY4264544.1"
                     /translation="MIVTRFAPSPTGFLHLGHACAALTAFEAARAADGSFHLRIENID
                     LTRCRPDFEAAIFEDLRWLGLSWPVPVRRQSEHFADYRSALNGLDSLGLLYPCFCTRK
                     EIAEEIARADFAPEAAELSPEGPLYPGICRTLTTEIRRARIARGDAYALRLDVAKALA
                     SLGRELIFDERGHGANGESGIQAARPEIFGDVVLARKEMPTSYHLAVVVDDALQGVTL
                     VTRGEDLFPATHIQRLLQGLLDLPTPVYAHHRLILDRSGRKFSKRDRAPTLRNLRQSG
                     ATPEDVRVMIGL"
     gene            11578..12264
                     /locus_tag="VGM72_04455"
     CDS             11578..12264
                     /locus_tag="VGM72_04455"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY4264545.1"
                     /translation="MSTLDGPRLPPVRPPATHLVVLCHGYGSDGNDLIGLAPHWRNAL
                     AGAAFVSPNAPERIAGAGYQWFPLSRIDPQETLRGTEAAAPKLNAFIDEELARLQLTG
                     DKLALVGFSQGTMMSLYVGLRRNPAPAAIVGFSGMLVGAESLPHSGNAPPVLLLHGDM
                     DQVIAPQALFMSANALGAAGVSVQWHMSKGLAHGIDATGLALAGQFLSDAFAGRAPRT
                     GPAAPSNLRG"
     gene            complement(12247..14022)
                     /locus_tag="VGM72_04460"
     CDS             complement(12247..14022)
                     /locus_tag="VGM72_04460"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4264546.1"
                     /translation="MAEAARLIPPDYDSRTIVGAGDEALARVEAATLAVATIAARLRS
                     NKVLQRSLRAWKRGDTVKTAKLALEATEADESNAQAFHLLALALDKLGHLHKALVTYE
                     RAFALDPDDTDLLLNLGLTAWNLDLLDGAERMFRLFIEKCPDHPAGYNNLGSIQRDKG
                     DLTLAIETLRNAILRMPDSAMLWNSLATALAEEGRAEESLPFYHEAMRLDPKFARVWH
                     NLGYSYMHLGRLEESLHAYDCALAIASSAHERVESRHSRSICLIGMGRLEEGFKDYEI
                     RHAPEFRTWLLHYTKAPLWRGEPLAGKRILIVGEQGLGDELMFANILPDVARAVGDDG
                     QLQIAVDPRLIPIFQRSFPKAEVGSYDDGKLEGRQVRIFHWARENGDPDFYAPIGTPL
                     HFFRKNVSDFPHEAFLKADPEKRAGFRARLAGLGPGPYVGICWRSMVMGAKRAKYYSA
                     IDSWGPILNVPGVTFVNLQYGAVAPELQHAREKLGIAIHHFDDVDLRNDLDGAAALTD
                     ACDLVISAPTAAAAMAGALGRETWFMAAGRVWPQLGTDHYPWYRSTRVFACEKFADWR
                     TLMPEVARALSDFVAQAHDASSPQI"
     gene            14188..14613
                     /gene="flbT"
                     /locus_tag="VGM72_04465"
     CDS             14188..14613
                     /gene="flbT"
                     /locus_tag="VGM72_04465"
                     /inference="COORDINATES: protein motif:HMM:NF001995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048027 - mRNA 5'-UTR binding [Evidence
                     IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis repressor FlbT"
                     /protein_id="HEY4264547.1"
                     /translation="MPLKLSLKPGEKFVLNGAVLANGDKRASFVIQNKACILREKDIM
                     QAEAADTPAKRIYFPIMMMYLDDEEADVYYSQFALRMTEFMDAIRDRKTLTTCVEISR
                     EVMSGAYYKALVKCRNLFEFEQERLNYGTSGLSKNATRD"
BASE COUNT         2643 a         4430 c         4708 g         2881 t
ORIGIN      
        1 gcaatcgctg atgcgaagca gcggactgga tttcacggcg ctcgatctca tcgcggtgac
       61 caccggccct ggtacgttca cgggacagcg ggtcgggctg gcgtttgcgc gcgctttgcg
      121 cgtggcactg aacaagcccg caatcggcgt tacgacgctg gattgtctgg ccgaggaagc
      181 attggtcaga cagccccgcc catgggccat cgccgccgcc gatgcgaagc gcgaagagat
      241 ttacctcggc gcgcgatccg cgcgaggtga gacgttgatt gcaccggagt tgattgcccg
      301 cgacatggtt gcggcccgcc tgagcgctct ggcgaacctc cacgggaatc gcgtcgcgct
      361 cgcggggacg gcagctgagg ctgttgcgcc cctgctggcg gagattggat ggcagtgcac
      421 cgacagcctg gtgcgccagc ccgacgcgat ttttgtcgcc gggctcgtgg ccgctgaagc
      481 acgcgcgggc gtccatgatc cgataccgcc gccgccaaaa ccgctctatc tgcgtgctcc
      541 cgacgcgaag cttccggggc gcgcgctatg acggccatca tccggggggc gatgcccggc
      601 gatgcgccgt tgctcgcgcg cctccacggc gcctcattcg acgagccttg ggacgcggac
      661 tcgtttcgcc gattgatgga gcggcccgga gcgttggcgt ttttaggcgc gaatgaagct
      721 gcgactgatt tgcaggcttt catcctggta caagtcgcgg ccgatgaatc ggaaatcctc
      781 agcattgcga cgctgccagc cgcgcgtcgt ctgggacttg cgcgcgcgct cattatgcag
      841 gctgcggcgg aagcgtcaaa aaacacggca atagcgatgt tcctggaagt tgccgaggac
      901 aatttcgccg cgctcgcgct ctaccgcgat tgcgggttca cggcgcaagg acggcggcgt
      961 gcctattacg tccgccgcgg aggctcctct gcggacgcgc tcacgctgcg tgcgcggttg
     1021 ccgttgaaac aagtcatggg aatgatgcgc ggtctcgatt agagtaatcg cttggagccg
     1081 ggagatcccc acgtgaaccg gactgaagaa aatctcaatc gcatcgaaaa ggcctgcaac
     1141 gagaagggtc tgcggatgac cgagcagcgg cgcgtgatcg cgcgcgtggt cgcggaagcc
     1201 tatgaccacc cggacgcgga agagctgtat cgtcgcgcca tctcggtcga tccgcatatt
     1261 tccatcgcca cggtttatcg caccgtgaag ctgttcgagg atgctgggat tctcgaacgt
     1321 catgattttc gggacggccg ctcgcgctat gaggaaatca gtgaacatca cgatcacctg
     1381 atcgacatct ccacgggccg cgtgatcgaa ttcgtcaatg aagatatcga gaagctgcag
     1441 cggcgcgtcg ccgaggagct gggcttcgaa ctggtcgatc accgcctcga actttacggg
     1501 atccccaagg gcggcaagaa gcccggcccg ccgacccaat aaatagcgcc gcttgggcgc
     1561 gtgagcttgt gaccatggcg agtcttcgcg ccttttgcat tcttttcgtc tttgccaccg
     1621 caacattggc cgggattccg ctgcaatggc tggcggtcaa actcggtctc aacgcggcgc
     1681 gcacatttcc caggctctat caccgcttcg tgtgccggct gttcggcgtg cgcgttgccg
     1741 ttaccggcgt gcctctgcgc ggaggcgggc tgatggcggc caatcacacc ggctggctcg
     1801 atattccgat tctctccagc atcgcgccgg tgtccttcgt cgccaagtcc gaagtcggac
     1861 gatggccgtt cttcggcaca ctggcgcggc tgcagcgcac gatctttgtc cgtcgcgaaa
     1921 aaagcaaagc ggcggaagac cgcgacaata tcaagatgcg gctgatcgaa ggcgatacgc
     1981 tggtgatctt tccggaaggg acctcgagcg acggcaatca tgtgcttgca ttccgcagcg
     2041 cgctgctgtc ggcggccgaa ctcggtctcg gcgaagacgc cggccaccat cgtctgcagg
     2101 tgccggtcca gccggtgtcg gtcgcttata tcggcgtgca cggcatgccg atgggccggg
     2161 aaatccgccc ttttttcgcc tggtacggcg atatggaact ggtaccgcat ctctgggagg
     2221 ccctgaagac aggaccgatc gaagtggcgg tggagttcca cccgcctttg actattggtc
     2281 aggcaggggg gcgcaaagcc ttggccgccc gggcagaggc cgcggtccgg tctgggctga
     2341 tccgggcttt ggcgggctcg aacgcggcgg tcgcaccgcc gcaacaggat gaagcattgc
     2401 tggaggccct cagcgaagcc gaggatgaaa acgcggatgc cgcctgatag gaccttcacg
     2461 ctcccaggtt gtcttgacgc gcgcgtttgc gcataaacat tccctcggtc atcgctgaag
     2521 tccgcatgaa gacgctctac atcaagacct atggctgcca gatgaacgtc tatgattccg
     2581 cccgcatggc ggatcttctg gcgccgctgg gttatatgcc cgccgatcgc ccggacggcg
     2641 cggacatggt catcctcaac acctgccaca tccgcgaaaa ggctacggag aaggtctatt
     2701 ccgagcttgg caaattgaag cagatgaaaa cgctgcgcgc gcaaagcggc aatccaatgc
     2761 tggtcgcggt cgcggggtgt gtcgctcagg ccgagggcgc ggagatcatg gcgcgccagc
     2821 ctgcggtgga catggtcgtg ggaccgcaat cctatcatcg tctgcccgag atgatcgccc
     2881 gcatcgcccg tggcgcaggc cacgcgatgg agaccgattt tccggccgaa gacaaattcg
     2941 actcgcttcc ggaagaacaa gccgtctcgg gccccgccgc ctttctcacc atccaggaag
     3001 gttgcgacaa attctgcacc ttctgtgtgg tgccctacac gcgcggcgcg gaattctcac
     3061 ggcccgcgat gcagatcgaa gccgaggcgc ggcgtttggc ggacaaaggc gttcgcgaga
     3121 tcacgctgct cggccagaat gtgaacgcct atcgcggcgc cggccccgac ggttcggtct
     3181 ggaatctggc ggacctgatc cggcatctgg cgcgcatcaa gggcgtcgcg cggctgcgct
     3241 acaccaccag ccatccccgc gatatggacg tttcgctgat cgaggcacat cgcgatgtcg
     3301 aagcgttgat gccttatctt catctgccgg tgcagtcagg ctccgaccgt atcctcaaag
     3361 cgatgaatcg cggacacacg attgcggaat atcttcgcat tgtcgaacgc atccgggtgg
     3421 cgcgccccga catcgcgctg tcgtcggatt tcattgtcgg ctttcccggc gagaccgacg
     3481 ccgatttcga gtccacgatg agcctcgcgc gcatggtcgg ctatgccggg gccttcacgt
     3541 tcaaatacag tgcgcgtccc ggaactccgg cggcggcaac gcccaaacag atttccgatg
     3601 cggtgaggga cgaacgtctg cggatattgc aggagttgat cgccgcgcag caggccgcct
     3661 tcaaccgcag ctgcgtgggg cgtgtattcc cggtgctgtt cgagaaaccg ggcaagaagc
     3721 cgggtcaggc ggtcggacga agcccctatt tgcagcccgt ccatgtcgag ggtgcgcatg
     3781 ctctgatggg agagattcgc gacgtgcgcg ttgctcagac cctgccgaac agtctcaaag
     3841 gcgtcctcgc gaacgcccag cacccggtgc cgtcccattg aaccggcgcc acggcacgaa
     3901 cgcgccggac gccgccgccc atgagcaggt gacgttggaa ttcacgcgca atgcgttgat
     3961 gcccgcgttg acgggcaatc acagccgcaa cctcgttcgg ctggagcaaa aactcggcgt
     4021 gaagctggcg cagcggggga atctgatcgc ggtggagggc gcccccgaag cacgcgcgca
     4081 cgccgcgacg attttgcgcg cgctttacga acgtctcgaa gccggagagg aactgacgat
     4141 gggcgatatc gatgccgaaa tcggcttcgc ggcgggcccc tcgcatgaat tgccgcaacg
     4201 cgatagagcc ggcacgctgc gcaccgcggc gggcaagctg acgcggccgc gttcggcggc
     4261 gcaaggcgcc tatatcgagc tcatgcgaaa tcgtgaactt gtgttcggcc ttggtcctgc
     4321 aggaaccggc aagacctatc ttgcggcggc gttcggcgcg catctcctgc atgagcggcg
     4381 cgtcgagcgg cttattcttt cgcggcccgc gctcgaggcc ggagaacgtc ttggctttct
     4441 gcccggcgat ctgaaggaga agatcgatcc ctatttgcgt ccgctttacg atgctctgtt
     4501 cgaggtgctg ggggagcgtt gcaccaagct gatggagacc ggaatcatcg aagtggcgcc
     4561 gctcgcgttc atgcgcgggc gcaccctgtc gcgcgccttc gttatcctgg atgaagcgca
     4621 gaattgcacg ccggcgcaga tgaagatgtt cctgacccgg ctgggcgagg aatcgcgcat
     4681 ggtggtgacg ggcgatccct cgcaaagcga tctgccgaac gggcctgaag gcctcgattc
     4741 cgcggtgcgc tatctggacg gagtggaagg cgtgggcatt gtgcgtttca cagcggccga
     4801 tgtcgtgcgc cacccgctcg tgacccggat cgtcgcggcc tatgatgctc acgaaccgga
     4861 tacgaaagcg cggtcgcgcc aagtcagacg agacctggaa tgatcggacg cgcggcggcg
     4921 cgcgccggcc agcccgcgat tgtctttttg atcgaagagg cgcgctggcg cgaagatgcc
     4981 gccgtcttgc gtgtcatccg ccgggccgta cggctggggc tccccaaaaa aaagaccggt
     5041 gaatcggtca cgatcctgtt gaccggcaat gcgaaattgc gcgcgctcaa tgcggcgttc
     5101 cgcgcccgcg acgtgccgac caatgtcctg tccttcccgg cggccaaagg cgacccgtcc
     5161 tctttcggcg atatcgccat ggcctatggc gttgtgaggc gcgaagcccg cgcgcaaaaa
     5221 aaggattttg ccgcccatgc cgcgcatatc gctttgcatg ggaccttgca ccttctgggt
     5281 tacgatcacg aagaatcgaa ggacgcggaa attatggaaa gcctggaagt tcggcttctg
     5341 gccacattag gcatcgccga tccttaccgc ccgcgtccct atacgaaggg acgcaagaaa
     5401 gcctaaactc acgccatgcc tgccgattcc gatacgaagt ccggacccgt tgcggtcctc
     5461 gccagccccc cccagaccga aactgccgac gggcgcccag caggcctgcc ggcgcgcgcg
     5521 ttgttgagcg aaatcgcgac ccggctcgcc caggcagtcc ggggcctgcg ccgggactcg
     5581 gccatgcgcg agagcctgga agagatgatc gaggagagcg agcgcgaggc caaggaattg
     5641 acctcgcaag aacgcatgat ggtcaccaat cttctgacct tcggcgagct ttcggtctcc
     5701 gatgtgatgg tgccgcgcgc cgatatcgtc gcggtggagg agcagatagc tttcgccgat
     5761 ctggtggcgc tctttcgtga agcgcagcat tcgcggcttc cgatctatcg cgagaccttg
     5821 gacgatccgc tcggcatgct gcacatcaag gatgtcttcg ccctgatcga aacgggagag
     5881 gacggacaat tgcgctggac gccgacgcct atcaccaaac tgaagcgcga agttctgttc
     5941 gtgcccgcgg ccatgcccgc gctcgatctc ctcttgaaga tgcaggcgac ccgcattcat
     6001 ctggcccttg tggtcgatga atatggcggc accgatgggc tcgtctcgat cgaggacctg
     6061 gtggaggaaa tcgtcggcga tatcgacgac gagcacgatg ttgaggaagt ccccgacatc
     6121 gtggcgcgcc ccgatggcaa atatgatgcc gatgcgcgtg tcgacctcga cgacttcaag
     6181 gagcgcaccg gtatcgatct cacgggtatg caaaccgaag aggacgtgga cacgcttgcc
     6241 ggaatcgttg ccgcggcctt gggccgcgtc cccgtgcggg gcgagatcgt cagccatggc
     6301 ggcgttgagt tcgaggttct ggaggccgat ccgcgccgcg ccaaacgctt gcgcattcgg
     6361 gcgctgcccg tgacggcgcc tgtcgcggcc ggttgacctt gcgcctttcc acggcctttc
     6421 tggatcggac tgccgcttcg ctccaacgcg tgcgcgggtt taagcgggtt gccgccgccg
     6481 ccggtctggg tgcgatgggc gcgctgggtt tcgagccctt tcgcgccttt ccgctgctgc
     6541 tcctgtccta tgcggggctc gtgcttctgc tcgatggcgc aagcgcaagc cctcggcgtc
     6601 tgcgccacgc ctttggcatc gggtgggcct atggttttgg atttttcctc gtcggactgc
     6661 attggatcgg ttacgcgttc ctggtcgatg cggccgccca tggctgggaa attcccttcg
     6721 tcgcaatcct gtttccgggc gggcttggac tcttttttgc gctaggcgcc gcgggttgca
     6781 tgctcagatg gcgcccgggc gtgcagcgga tatttctttt cgcgctggtg ttcgcgatcg
     6841 tcgagtggct gcgtggccat gtgctgacgg ggtttccctg gaatctcccc gcctatggct
     6901 ggggagcctc gctgggggtg cttcagagcg cgtcggtttt cggcgcctat ggcctttcgc
     6961 tgctgacact gttgtttggc gcgtcgctcg cgatccttcc cggtgcaagc gcgcaggcac
     7021 gctggttgcc ggtgacgatc acgttattct tcctcgcgct ctgggcggat ggggaattgc
     7081 ggctgttcac agccaccgat gcggtcgtcc ccggggttca tttgcgcctc gtccagcccg
     7141 acacgccgca aagtgagaaa tatgttcaac gctatatgga gcgcaattgg tggcggctga
     7201 tcgatttgtc cgccgcgcct gcgcccattg ctccgaccca cattatctgg ccggaggccg
     7261 cccccccctt cgtgctcgcg cgcgcacccg gagcgctgtc cgagatcgcg cggctgaccg
     7321 gcgattcgcg ggtgctgcta acgggtgaag tgcgaattgc cggcgagggc ccggccgcgc
     7381 gcttctacaa ttcgttcgcg atgttcggac ctcacggaaa attgctcgcc acttcggaca
     7441 aattccatct ggtgccgttt ggcgaatatc tgcccttcga atcggcgctt cggtccatcg
     7501 gtcttacgga aattgccgcc aataccggct tttcgtccgg cccgggaccg cggactttct
     7561 ccgtacccgg agcgccgccc atggggcctc tcatctgcta tgaagtgatc ttcccccaag
     7621 cggtgaccgg cgacccgcgg ccatcctggc tggttaacat gacagacgat tcatggtttg
     7681 gtccaaacgc cggaccgatg cagcattttt tgatcgcgcg cgtgcgcgcc attgaagaag
     7741 gtttgccgat cgcacgcgcc gcgaatagcg gtatttccgc ggtgatcgat ggttacgggc
     7801 gtgtgcaggc gcggctcgat cttgggttgc gtggtcatct tgattcagac ctcccgattg
     7861 ccctccccgc gacaccattt gcgcgctacg gaaatttcat aatgctggcg ctctatgtga
     7921 tttgtgcagg tgcggcgctt tggcccccaa gcctttccaa ggcttgacgt attggggtgc
     7981 gagagctttt cttcgcccat tccaatttgt acagggtcaa agaaaggttt ggggaagtgc
     8041 caaagaaaca ggcgaatccg gtcgacgggc atgtcggaca ccgcgtgcgg ctgcgacgca
     8101 tgctgatcgg aatgagtcag gagcgtttgg gcgatttgct cggtctcaca tttcagcaag
     8161 ttcagaaata tgaaaaggga ataaatcgca ttggggcagg acggctcttc gaggttgcag
     8221 ggattctcgg agttccgatc agcttcttct acgaagatgc tcacgccggc gcggcgtccg
     8281 tgagcggctt tgccgaagga gacgagccgc ctcctgtgat ggaatttctt tccagtggag
     8341 aaggcctcca gctgagcctc gcattcatgc gcatcaagga tggcaaggtc agacggcgca
     8401 ttctcgatct ggtccgatcg ctggcgaacg aggacggtga catcaactag atactgccgg
     8461 cgggcttgtt cccggcgtca gctcttgcgg gaggctttct cgtcgatgcc cgattgcgct
     8521 tcgcgttgcg cgagcgcgtc gtaaacctcg tcgggggata tctcgcacgc cgcccatagc
     8581 accatcaggt ggtagagaag atcggcggac tcgtttgcca ccgccgtctt gtcttccgcc
     8641 accgcggcaa ggcttgtctc gatggcttct tcgcccagct tcttggcgca tttcagccga
     8701 ccggctttga gcagcttggc cgtgtaggaa agatttgggt cctcgccttt gcgggcgagg
     8761 acgcgctgaa acagccggtc gatcggatga gtcgtcatga ggctcagttt ggagcgcgaa
     8821 gcgggcgcgt gcaatcgctc aggtgccttt ctgcgatgca atccagcggt tgatcttcgc
     8881 gtccaggatc ggaagcggca tggcgccatc cttcagcacc gcctcatgga acagcctgat
     8941 gtcgaatttg gggcccagcg cggctttcgc acgattgcgc agatcgagga tctcgcgttc
     9001 gccgatccga taggagagcg cttgcgccgg aatggcgatg tagcggtcca cctcttcgga
     9061 cgcttcgttc tccgacagtc ccgcatgggt ttggaggaac gcaatcgcct ggaagcgcgt
     9121 ccagccgagc caatgaatcc cggtatccac caccagccgg ctgctgcgcc aggcctcgaa
     9181 ggaaagcgcg ccgaatttct gatagggatc ggtataaagc cccagatcgc tacccaatgt
     9241 ttcggcatag agcgcccagc cttccgtgaa ggccgtttca tagccaaagc gccggaatcg
     9301 cggcagcgcg ttgttttcca tgctgatcgc cacctgcata tgatggccgg gaatgccttc
     9361 gtggatcgcg agcgcttcac tcgtgtaggc cgggcgcgcg ccgagatcgg atgcgttcag
     9421 agagacgatg cccggacgtc tgccatcggc tgaagcggac gtatattccg ccgccgggcg
     9481 gtcgggcgcg acatagggtt cataaaaacg gaacacaaga tccgacttcg gcttggtgat
     9541 gaaaagctgg ctcagatgcg cgtcaaccgt attcttcacg cgggtgaatt cgccaagcat
     9601 cgcgtccttg tccttgaatt tgaaccgcga gtcgttgtgc aggaagacac ggaaatccgc
     9661 gagcgaaccg gagaagcccg cctcgttctt gacgcgctcc atcgccgctt cgatgcgtgc
     9721 cagatcggac agtccgagcg cgtggatggc atcgggtgtc aaatccgaag tggtttcgct
     9781 cctgacgaga taaagataag tctccttgcc gcccggaatg gcggagaggc cgatgctctc
     9841 tcgcgcgtgc ggtagatatt cgtttttcag gaagtcgagc agccggcgat aggctggcgc
     9901 caactgccct tccacggctt cgcggtaggc gccctcgatg cgtgcgcgtt ccgcgccggc
     9961 gatcgggtcc ggcatggttt tcaggggagc catgaagagg ccgtcatcgg gatccccatt
    10021 ggcgaaaatc tcggtttgcg cgatcatgcg cgtcacgatg gcgcgcggct gcacgatgcc
    10081 tcgcttgatg ccttcgcgca ttttgccgat ggccgcatcg atgccgttcg tgaagcgcag
    10141 catgcggccg atggcgtcgt catagtcctt ggatcgggtc catgggtgct cgctgcgcga
    10201 ttgcatctga cgggggaaga tgatctgcgc gccgtcgaac tggttgagcg cgagaagctg
    10261 gaaattctcg gcgatggcgg gactgagttc gcgggcttcg tcgtccagcg accagccgaa
    10321 gatctcgtag gaaagcaagt cctgcgcctt cagtatgagc gcatcgatgg aagcgagctt
    10381 gccgcgtgta ttttcgttga tgcgctgccg cgcacgcagc gtgtcggcgt tgcgcttttc
    10441 gtcataaacg ccggagcttt cgtcggggcg caatttgcct tcggagacgc gataatcgcg
    10501 catcagcctg gagagatcgt caccggcatc ggcatgcacc gttccggcgg cgaagaaaca
    10561 tgcgaagccg gcggcgataa cgagcttgaa attattcatg cgcgcctcat cgcataagct
    10621 tgcagcagaa tgacggcgca cgccatgggt tgaacttttc gtgacgcgaa ggaagattcc
    10681 tacagcccga tcatgacgcg gacatcttcg ggcgtggcgc ccgattgccg caaattccgc
    10741 agcgtcggcg cgcggtcgcg tttggagaat ttgcgccccg atcgatccag gatcagccga
    10801 tgatgggcgt agaccggcgt cggaagatcg agcaggccct ggagaaggcg ctgaatatgg
    10861 gtcgccggga acaggtcctc cccgcgtgtg accagagtga ctccctgcag cgcatcgtcg
    10921 accacgaccg ccagatgata gcttgtgggc atttcctttc gcgccagcac gacatcgccg
    10981 aagatttccg gtcttgcggc ctgtattccg gattcaccgt tggcgccgtg gcccctttcg
    11041 tcaaagatca gttcgcgtcc cagactggcc agcgcctttg ccacgtccag ccgcaacgcg
    11101 taagcatcac cgcgcgcgat gcgtgcgcgg cggatttccg tcgtcagggt gcggcagatt
    11161 ccgggataaa gcggtccctc gggagacaat tcggccgctt ccggtgcgaa gtctgcgcgc
    11221 gcgatctctt cggcgatttc ctttcgcgtg cagaagcaag gatagagcag gcccagcgaa
    11281 tcgagcccat tcaaggcgga cctgtaatcg gcaaagtgtt ccgactggcg gcgaactggc
    11341 accggccagc tcagtccgag ccagcggaga tcctcgaaga ttgcggcctc gaaatcgggg
    11401 cggcagcggg tcaggtcgat gttctcgatg cgcaggtgga agctgccgtc ggcggcgcgc
    11461 gcggcctcga aggcagtgag cgcggcgcag gcatgaccca gatgcagaaa tccggttgga
    11521 cttggcgcga agcgggtgac aatcatgggg cgggaatcta cacggaaaac ctcaagcatg
    11581 agcacgctcg acggcccgcg gctaccgccg gtgcgcccac cggccaccca tctcgtggtg
    11641 ctgtgccacg gctatggctc ggacggcaac gatctgatcg gcctcgcccc gcactggcgc
    11701 aacgcgcttg ccggcgcggc tttcgtgtcg cccaacgcgc cggagcgcat cgccggtgcg
    11761 ggctatcaat ggttccccct gtcccgcatc gacccgcagg aaacgctccg tggcaccgaa
    11821 gcggcggcac cgaaactgaa cgcctttatc gacgaagaat tggcgcgtct ccaactcacc
    11881 ggcgacaagc ttgcgctggt cggattcagc cagggcacca tgatgtcgct gtatgtcggc
    11941 ttgcggcgca atccggcgcc ggccgcgatc gtcggctttt ccggcatgct ggtgggtgcc
    12001 gaatccctgc cgcactccgg aaacgcgccg ccggtgctgc tgctgcatgg cgatatggac
    12061 caggtgattg ccccgcaggc gctcttcatg tcggccaacg cgctcggagc ggcgggcgta
    12121 tccgtgcagt ggcatatgag caaaggtctc gcgcacggca tcgacgccac gggacttgcc
    12181 ctcgccgggc agtttctctc cgacgcgttt gcgggccgcg cccctcgtac cggtccggcc
    12241 gcgccctcaa atttgcgggg atgacgcgtc gtgggcctgg gctacgaaat cgctcaaggc
    12301 gcgcgccacc tcgggcatca atgtgcgcca atcggcgaat ttctcgcagg cgaaaacgcg
    12361 ggtgctgcga taccaggggt aatggtcggt gccgagctgc ggccagaccc ttcctgccgc
    12421 catgaaccag gtctcgcgtc ccaaggcgcc cgccatggca gccgccgcgg tgggtgcgga
    12481 gatcacgaga tcgcaggcat cggtcaaggc ggccgcgccg tctaaatcgt ttctgagatc
    12541 gacatcgtcg aaatgatgga tggcgatgcc gagtttttct cgcgcatgct gtagctcggg
    12601 tgcgacggcg ccatattgca aattgacaaa cgtcacgcct gggacgttca ggatcggtcc
    12661 ccaggaatcg atggcgctgt aatatttcgc gcgcttggcg cccatgacca tcgaacgcca
    12721 gcaaatgccg acataggggc cggggccgag tccggcaagc cgcgcccgga aacctgcgcg
    12781 cttttcggga tcggccttca ggaaggcttc gtgcggaaaa tccgagacgt tctttcgaaa
    12841 gaaatgcaga ggcgtgccga tgggcgcgta gaaatccgga tcgccattct cgcgcgccca
    12901 atggaatatg cggacctggc gcccttcgag cttcccgtcg tcataagagc cgacttcggc
    12961 cttgggaaag gaacgctgga aaatcggaat gagtcgcgga tcgacggcaa tttggagctg
    13021 gccgtcgtcg cccacggcgc gtgcgacatc gggaaggatg ttcgcgaaca tcagctcgtc
    13081 gcccaagcct tgttcgccga cgatcagaat acgcttgccc gcgagcggtt cgccgcgcca
    13141 cagcggcgcc ttggtgtaat gcagcagcca ggtacggaat tccggggcat ggcggatttc
    13201 atagtccttg aaaccttctt ccagccgtcc catgccgatc aggcagatgc tgcgtgaatg
    13261 acggctctcc accctctcat gcgcgctgga cgcgatggca agcgcgcaat cataggcgtg
    13321 cagggattct tccaggcggc cgagatgcat gtagctgtag ccgagattgt gccagacccg
    13381 cgcgaatttg ggatcgaggc gcatggcctc atggtagaag ggaaggcttt cttcggcgcg
    13441 gccttcttcg gcgagcgcgg tggcaagcga attccacagc atcgcggaat ccggcatgcg
    13501 caggatcgcg ttgcgcaggg tttcgatcgc caaggtgaga tcgcccttgt cgcgctggat
    13561 gctgccgaga ttgttgtagc cggccggatg gtcgggacac ttctcgatga acagccggaa
    13621 catgcgctcg gcaccgtcca gaagatcgag gttccacgcg gtgaggccga gattgagcag
    13681 cagatcggta tcgtcggggt cgagcgcgaa ggcgcgttca taggtgacaa gcgccttgtg
    13741 cagatggcca agcttgtcga gcgcgagagc gagcagatgg aaagcctggg cgttggattc
    13801 gtcggcctcg gttgcctcga gcgccagttt ggcggtcttg acggtgtcgc cgcgcttcca
    13861 ggctcgcagc gaccgctgca gcactttgtt cgagcgaagc cgggctgcga tcgtggcgac
    13921 cgccaaagtc gccgcttcga cgcgcgcaag cgcttcgtcg cccgctccga cgatggtgcg
    13981 ggaatcgtaa tcgggcggta tgagccgcgc agcctctgcc atggtgcttc tccttgagcg
    14041 tccataaaac agcaagcgtg gataaatttg cgtaaacagt ttgcgttcgc atcgcatttt
    14101 gaagggagtg ttaaccagtc ccggcctaag gtcccgccga cattgcatcg ccacccgcgc
    14161 aaaatgcgca acgtccgagg acatccaatg ccgcttaagc tgtcgttgaa acccggcgag
    14221 aaattcgtct tgaacggcgc agttctggcc aatggcgaca agcgcgccag tttcgtgatc
    14281 cagaacaagg cctgtatcct tcgcgagaag gacatcatgc aggccgaagc ggcggacacg
    14341 ccggccaagc gcatctattt ccccatcatg atgatgtatc tcgatgacga ggaggcggat
    14401 gtctattaca gccagttcgc gcttcgcatg accgagttca tggacgcgat tcgcgatcgc
    14461 aagacgctga caacctgcgt cgagatcagc cgcgaggtga tgagcggcgc ctattacaag
    14521 gcgctggtca aatgcagaaa tcttttcgag ttcgaacagg aacggctcaa ctatggcact
    14581 tcaggcctat caaagaacgc aacgcgcgac tgagaatacc agaggcgcgg aataccgttt
    14641 gttcggtcag gtgacgggcg cg
//
LOCUS       DASYEG010000111         7036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3223976, whole genome shotgun sequence.
ACCESSION   DASYEG010000111 DASYEG010000000
VERSION     DASYEG010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3223976"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VGM72_04470"
     CDS             complement(<1..237)
                     /locus_tag="VGM72_04470"
                     /inference="COORDINATES: protein motif:HMM:NF040598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpp/OprI family alanine-zipper lipoprotein"
                     /protein_id="HEY4264548.1"
                     /translation="MSRTFLPLVMGTALLTAGCATNDDLKAVQATADSARMQAEQARA
                     TADQAIAASQMAGQRADQANATAQQAQATANGAQT"
     gene            655..2253
                     /locus_tag="VGM72_04475"
     CDS             655..2253
                     /locus_tag="VGM72_04475"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY4264549.1"
                     /translation="MSATQSALRDLLLSKEAQSQQSAAALPDPSFAFYKARNFMPVWS
                     GGAEQEDASAKVHAILGRAHEQGLRDEDYNMSQDESRRTSGAEAAAYDLALTRTVLAY
                     SRDVRTGHVWPGDIYDDVILPVPGFNAATDLTKALADHAIAKYLADLSPPHREYGLLA
                     VALAHYRAIAGQGGWPSLPGQTEIKLDGKDPRASLLAARLAFEDPALAATPDPSAGEL
                     REAVKRFQTRNGLAGDGRVGGETLTSLNIPASYRAAQIAANMERWRWLPRFEDRYIAV
                     NVPDQSLEFVRDGIPTLSSRVIVGQKTSPTPITRTEIVAVVANPPWNIPGDIAARDLL
                     PHLVKSANYLASKHMVVTNGPPGDPYGRTIKWASVVPAEFPYAIRQLPGPATALGAVM
                     LDSPNDFDVYLHDTPNKKRFESNEREISNGCVRVQQILSLASLALTGDPAKGMDRLNR
                     VIKTHETQRLTLDHSLPVYFLYWTAIPAADGSVGFRPDRYGRDARLIAALTKNDRSIP
                     AIEIPMNASGDMEQNAPDADDPSP"
     gene            complement(2286..5039)
                     /locus_tag="VGM72_04480"
     CDS             complement(2286..5039)
                     /locus_tag="VGM72_04480"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:NF003467.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEY4264550.1"
                     /translation="MAVDTFSAAVMDGAQPLDGDALRRDLTALARASGGDRAVLRKAA
                     LVSIRAAFLAARERIKAGMESGVSPGQAVARALSALQDITIQVLYDFAVRHFYRAPNP
                     TTSERLAVVATGGYGRGLLAPASDIDLLFIRPFKETAWDESVIEFILHMLWDMGLKVG
                     HATRSIPECVRLAKQDITIRTSLLEARYVWGDASLYEELRKKFWTDIATGNGQDFVEA
                     KLAERDARHLRQGESRYLVEPNIKDGKGGLRDLQTLYWIGKYLYRVEDTSDLVHHGVF
                     TKEEFKTFQKAEAFLWTVRCHLHYLTGRAEERLSFDVQPELAKRLGYSDPNLHRAVER
                     FMRSYFLVAKDVGDLTRIFCASLEEQNRKSHPSLTRLMPGFLKLRSSNDEFFVENGRL
                     AARPGLFREAPINLIRLFHVAESKGLDVHPNTLRRVTRQLDLIDGDLRNNAHANRLFL
                     DILCSRRDPERALRRMNEAGVMGRFMPEFGRVVALMQFNMYHHYTVDEHLLVAVGNVA
                     RFEHGEMRQELPLVSDLIKRVKSREVLYVATLLHDMAKGLPGDHSEVGAAMAEAVCPR
                     FGLSEADTDTVVWLVRNHLVMSDAAQKRDVSDPKTVQDFVRIVQTPERLRLLLILTAA
                     DIRAVGPGVWNGWKAQLLRELYFESETLMSGGDSSPARSTRIEDAKRALEERLKNFSD
                     AARQHALSRHYDSYWLAFDAEELEFHARLMQSADRTGEKLAVGSRGEISGAVTEIVIY
                     TADHPGLFSRLAGAISISNGSIVGAKIFTSSDGYALDVFQVQDAAGGPFGDGGRIDRL
                     RQTIIKTLAGEIRPRDVLAKRIPGRRAAAFKTKPKVNFDNEASTVSTLVEVESLDRVG
                     LLYEITRGLFEAGMTISSAIIATYGELAVDTFYVRDVFGHKVVHPQRLAAIEARLMKG
                     LDG"
     gene            5162..5923
                     /locus_tag="VGM72_04485"
     CDS             5162..5923
                     /locus_tag="VGM72_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4264551.1"
                     /translation="MTPAVKTVLITGGAKRLGRAIALHLGSAGWSVAVHYNVSQGDAE
                     ETVAALHGKGVHAAALNADLSMEEDSQRLIARANKAVGPLTALVNNASVFESDTLATM
                     TRESWEKHMETNLRAPIVLAQNFAEQLPEGAEGAIVNLLDQRLLKPTPQFLSYGVSKA
                     GLHWLTVTLAQALAPRIRVNAVAPGPTLRNARQSEAQFKKQQAATILGRGATPEDVAA
                     AALYLIESRAVTGQMIAVDGGQHLAWRTADVTGVE"
     gene            5920..6282
                     /locus_tag="VGM72_04490"
     CDS             5920..6282
                     /locus_tag="VGM72_04490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEY4264552.1"
                     /translation="MRNRSVLVLIRDLELLARIGVHGHEQGKPQPVRINIQLTGEIDS
                     TGDTLSNAIDYEAVADKIRAIIAAGHVNLAETLAERIAATCFDDKRVRHARVRVEKLH
                     ALPGAAAAGVEIERDAPV"
     gene            complement(6279..>7036)
                     /locus_tag="VGM72_04495"
     CDS             complement(6279..>7036)
                     /locus_tag="VGM72_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY4264553.1"
                     /translation="EIRDTPGHTRGCVSYVLGDESMAFTGDALLIRGSGRTDFQEGDA
                     RSLYRSVHLQLFTLPDSCLLYPAHDYRGLTVTSVGEEKRFNPRLGGALSENDFAGYMK
                     NLHLAHPKKLDIAVPANLKCGRPEEDHPMHDPDWAPLTCNFAGIWELQQPSWLEDNLD
                     AVQVLDVREPDEFTGPLGRIRGAKLIPLGELSARTGELAKDKPVVAVCRAGGRSAQAT
                     LILQQAGFERVANMPGGMLRWRGEGFPVEGGKS"
BASE COUNT         1445 a         2195 c         2087 g         1309 t
ORIGIN      
        1 ggtctgagcc ccattcgcgg tcgcttgcgc ctgctgggcc gtcgcattgg cctggtcggc
       61 cctttgtccc gccatctgcg aagccgcgat ggcctggtcg gctgtggccc tggcctgctc
      121 ggcctgcatt ctcgccgaat cggccgtggc ctggacggct ttaagatcgt cattcgtcgc
      181 acaaccggcc gtcagtagtg ccgttcccat gacgaggggc aaaaaagttc tgctcaaaac
      241 ccaatgcata tttttttctc cttctgtgat ccataggcac gtggcctacg tccccttgaa
      301 cccggcggag attagcactg gtgcggcaga aaaattgccc tcttaggcaa cgtcacaatt
      361 tttacacagg cggcaggcga ggaattcccc gattctccag tgacgaaaga aatcgaaatg
      421 gactctgcaa aacggactcc gtccgttcac ggagaaaact gccgttcgat catgaggctg
      481 tcgatgcatc caagggtttc cacgcgcccc gtgatgggtg aacggcaaca ggcttttagg
      541 gtccgggcgt ttcgcgcctc taccttcctg gtcgcagcac ttaatttggc agcgtgcgcg
      601 ggcaccggaa caagccggcc gccagccgtc accatcgtgc ctgagcaacg gcaattgtcc
      661 gccacacaat cggctttgcg cgaccttctg ttatcgaaag aggcgcaatc ccagcagagc
      721 gcggcggctc tgcccgatcc gtcatttgcg ttctacaaag cgcgaaattt catgcccgta
      781 tggagcggag gcgcagaaca agaagacgcc tcggcaaagg tccacgcaat cctcggccgg
      841 gctcacgaac agggcttgcg cgatgaagac tacaatatgt cacaggacga atcgcggcgc
      901 acatccggcg cggaagcggc cgcatacgat ctcgcgctca ccaggacagt gctggcctat
      961 tcccgcgacg tgaggacggg gcatgtttgg cccggcgaca tctatgatga cgtcatcctg
     1021 cctgtacccg gtttcaatgc cgccacggat ttgaccaaag cccttgcaga tcatgcgatt
     1081 gcaaaatatc tggccgatct gtccccgccc caccgcgaat acggcctgct cgcagtggcg
     1141 ctcgcacact accgcgcaat cgcggggcag ggcggctggc cttcgctgcc ggggcagacc
     1201 gaaatcaagc tcgacggcaa ggatccgaga gcgagcctcc tcgcggcgcg cttggcattt
     1261 gaagatcccg ctttagccgc gactcccgat ccttccgcgg gcgagttgcg tgaggccgtc
     1321 aagcgcttcc agacgcgcaa cggtcttgca ggggatggac gcgtcggcgg cgaaacgctg
     1381 acatcgctga acatcccggc gtcctatcgc gccgcgcaaa tcgccgcgaa catggaacgc
     1441 tggcgctggt tgccgcgatt cgaagaccgc tacatcgccg tcaatgttcc ggatcaaagc
     1501 ctggagttcg tccgcgatgg aattcccacg ctgagttcgc gcgtcatcgt cgggcagaag
     1561 acaagcccga ctccgatcac acgcacggaa atcgttgcgg tggttgccaa tccgccttgg
     1621 aacattcccg gagacatcgc ggcccgggat ctgctgcctc acctcgtcaa gagcgccaat
     1681 tatctggcat cgaagcacat ggtggtgacg aacggtccgc ccggcgatcc gtatggtcgc
     1741 acgatcaaat gggcaagcgt cgtgccggcg gaatttccct atgccatccg ccagctcccc
     1801 ggaccggcaa ccgctcttgg cgccgtgatg ctcgattcgc ccaatgattt cgatgtctac
     1861 cttcacgata cgcccaacaa gaaacggttt gaatccaacg aacgcgaaat cagcaatggc
     1921 tgcgttcgcg tgcaacagat tctttcgctg gcctcgcttg cgctcaccgg cgatccagcc
     1981 aagggcatgg atcgcctcaa tcgcgtgatc aaaacccatg agactcagcg gctgacattg
     2041 gatcattcgc tgccggttta ttttctctat tggacggcga tacctgccgc cgacgggagc
     2101 gtgggattcc ggcccgaccg gtacggccgc gatgcgcgcc tgattgcggc gctcaccaag
     2161 aacgatcgca gcataccggc gatcgagata cccatgaatg cttccggcga tatggaacag
     2221 aacgcgccag acgccgacga cccaagtccg taacgccggt cgcctaaacg aaaccagctc
     2281 cgcgttcagc cgtcgagccc cttcatcagg cgcgcctcga tcgcggcgag ccgttgcggg
     2341 tggaccactt tgtggccgaa cacatcgcgc acatagaacg tatcgactgc cagctcgccg
     2401 taagtggcga tgatggccga tgaaatcgtc atgccggcct cgaacaagcc gcgcgtgatc
     2461 tcatagagca gcccaacccg gtcgaggctc tcgacttcga ccagcgttga tactgtcgag
     2521 gcttcgttgt cgaaattcac tttgggcttc gtcttgaagg cagccgcgcg ccggccaggt
     2581 atccgtttcg ccaacacgtc gcgcggcctg atctcgccgg ccaaagtctt gatgatggtt
     2641 tgacgcaaac ggtcgatgcg tccgccatct ccgaacggcc cgcctgccgc atcctggacc
     2701 tgaaagacgt cgagtgcata accgtccgag gaggtgaaga tcttggcgcc aacgatcgag
     2761 ccgttggaaa tcgagatggc tccggcaaga cgcgagaaca atcccggatg gtcggcggtg
     2821 tagatgacga tttccgtcac cgcacccgat atctcgccgc gcgaacccac agccagtttc
     2881 tcgccggtcc ggtcagcgga ttgcatcagg cgcgcatgaa attccagttc ttcggcatcg
     2941 aacgcaagcc aatagctgtc atagtgacgg ctgagcgcgt gctggcgcgc ggcatcgctg
     3001 aaattcttta gccgttcttc caatgcgcgc ttggcgtctt cgatccgcgt gcttcgcgcc
     3061 ggagaggaat cgccccccga catcaaggtc tcggattcga aataaagctc gcgcaggagc
     3121 tgggccttcc agccgttcca tacgcctggt ccaaccgcgc ggatgtcggc ggccgtcagg
     3181 atcaagagga gccgcaaacg ctccggcgtc tgcacgatgc gcacgaaatc ttggacggtt
     3241 tttgggtccg acacgtcccg cttctgtgct gcgtcgctca tcacgagatg atttcgcacc
     3301 agccatacga cggtatccgt atcggcctcg gacaaaccga agcgcggaca caccgcttcg
     3361 gccatcgccg cgccgacttc ggaatgatcg cccggaagac ccttggccat atcgtgcagc
     3421 agcgtggcga catagaggac ttcgcgcgat ttcacgcgct tgatcagatc gctgaccagc
     3481 ggcagctcct ggcgcatttc gccatgctcg aagcgtgcga cattgccgac cgccaccaac
     3541 aaatgctcgt ccaccgtgta gtggtggtac atgttgaact gcatcagagc gacgacacgg
     3601 ccgaattcgg gcatgaagcg gcccataaca cctgcctcat tcatgcgccg aagcgcacgt
     3661 tcgggatcgc ggcgcgaaca gaggatgtcg agaaacaacc gattcgcgtg ggcgttgttg
     3721 cgcagatcgc cgtcgatcag atcgagctgg cgcgtgacgc ggcgcaacgt gttggggtga
     3781 acatcgagtc ccttggactc ggcgacatgg aacaaccgaa tcagattgat gggggcttca
     3841 cggaacagac cagggcgcgc ggcaagccgt ccattctcga cgaagaactc gtcgttggaa
     3901 gaacgtagtt ttaggaaccc tggcatcagc cgtgtcagcg atggatggga cttgcgattc
     3961 tgctcttcga gcgaggcgca gaatatccgg gtgagatcac cgacgtcttt ggcgaccagg
     4021 aagtagctgc gcatgaagcg ctcgaccgcg cgatgcaaat tggggtctga gtagccgagc
     4081 cgcttcgcaa gctccggctg cacatcgaag gacagccgtt cctcggcccg tccggtcaaa
     4141 taatggagat gacagcgcac agtccataga aacgcttcgg ctttctggaa ggtcttgaac
     4201 tcctccttgg tgaagacacc atgatggaca aggtcgctgg tatcctcgac gcggtagaga
     4261 tatttgccga tccaataaag agtttgcaga tcgcgcaggc cgcccttgcc gtccttgatg
     4321 ttgggctcga ccagatagcg gctctcaccc tgtctgaggt ggcgggcgtc acgctcggcc
     4381 agcttggcct ctacgaaatc ctgaccgttt ccggtggcga tgtcggtcca gaatttcttg
     4441 cgaagctctt cgtaaagcga agcatcgccc caaacataac gcgcttcgag aagcgaggtc
     4501 cggatggtga tgtcctgctt cgccaaacgc acgcattcgg gaattgaacg cgtggcatgg
     4561 ccgactttca ggcccatgtc ccacaacatg tgtaggatga attcgatgac actctcgtcc
     4621 cacgccgttt ccttgaaggg gcggatgaac aacagatcga tatcggaggc cggtgccagc
     4681 aatccgcggc catagccacc ggtggcgaca accgcaagac gctcggacgt cgtgggattg
     4741 ggcgcccgat agaaatggcg gacagcgaaa tcgtaaagaa cctggatcgt gatgtcttgc
     4801 agcgccgaaa gggcgcgagc gaccgcttgc ccggggctca cccccgattc catacccgcc
     4861 ttgatgcgtt cgcgcgccgc cagaaacgca gcgcgaatgg agacaagtgc ggccttgcgc
     4921 aacaccgcgc gatccccgcc ggatgcccgc gcgagcgccg tcaggtcgcg ccgcaaggca
     4981 tcgccgtcga gcggttgcgc gccatccatc accgctgcgg agaacgtatc aacggccatt
     5041 tcgagtcatc ggacctcgct gcggggtcat agaatgacgc tggccgtcca aacacaagac
     5101 gaatgatgcg ccacacgata agctttcgac aacgaggccc agccgatgta gagaatggag
     5161 catgacaccc gctgtaaaaa ccgttctcat cactggtggc gccaagcgcc tggggcgggc
     5221 gatcgcgctt catctcggtt cggctggatg gtccgtggcg gtccattaca atgtctccca
     5281 gggcgacgct gaggaaaccg tcgcagcctt gcatggaaag ggcgtgcacg cagcggcatt
     5341 gaacgccgac ctctcaatgg aggaagactc gcagcggctc atcgcgcgtg cgaacaaggc
     5401 ggtcggtccc ttgacggcgc tggtcaacaa cgcgtcggtc ttcgagagcg ataccttggc
     5461 caccatgacg cgcgaatcct gggaaaaaca catggagacg aacctgcgtg cgccgatcgt
     5521 gctggcccag aatttcgccg agcaattgcc ggaaggggcc gaaggcgcca tcgtcaatct
     5581 gctcgatcaa agattgttga agccgacgcc acaattcctc tcctacggcg tcagcaaagc
     5641 gggactgcat tggctgaccg tgacgctcgc tcaggcgctt gcaccgcgga tccgcgtcaa
     5701 cgcggtggcg ccgggcccga cgttgcgcaa tgcacgccag agcgaagccc aattcaaaaa
     5761 gcaacaggcc gccacaattc tcgggcgtgg cgcgacgccc gaagacgtcg cggcagcggc
     5821 tctttatttg atcgaatctc gcgcggtcac gggccagatg atcgccgtcg atggcggcca
     5881 gcatctcgct tggcgcacgg cagatgtcac aggtgtcgaa tgagaaaccg atccgttctg
     5941 gtgctgatcc gcgatctgga actgctcgcc cgcatcggcg tccacggaca cgagcagggc
     6001 aagccgcagc cggtccgcat caatatccaa ctcacgggtg agatcgattc gaccggcgac
     6061 acactgtcaa acgccatcga ttacgaagcc gttgcggaca agattcgcgc gatcatcgcc
     6121 gcgggccatg tcaatctggc cgaaaccctg gccgaacgga tcgcggccac ttgcttcgac
     6181 gacaagcgcg tgcgtcacgc gcgggtgcgg gtggagaaat tgcacgcgct gccgggtgcc
     6241 gccgctgccg gtgtggaaat cgagcgcgac gcccccgttt aggatttgcc accttcgacc
     6301 ggaaatcctt cgccacgcca gcgcagcatg ccccccggca tattggccac gcgttcgaac
     6361 cccgcctgct gcaggatgag cgtcgcctgg gccgaacgcc cgccggcccg gcagaccgcg
     6421 accacgggtt tatctttagc aagctcaccc gtgcgcgccg acaactcgcc cagaggtatg
     6481 agcttggcgc cacgaatgcg tccgagcggt ccggtaaact catcgggctc gcgcacatcg
     6541 aggacctgga ccgcatccag attgtcctcg agccatgaag gctgctgcaa ttcccaaatg
     6601 ccggcgaaat tacaggtcaa cggggcccaa tcgggatcgt gcatgggatg gtcctcctcc
     6661 ggccggccgc acttcaaatt ggcgggcact gcgatgtcga gcttcttggg gtgtgctaag
     6721 tgcaggttct tcatgtagcc cgcgaaatcg ttttccgaaa gtgctccgcc aagccttgga
     6781 ttgaaccgct tctcttcacc cacgctggtc acggtcagcc cgcgataatc atgggcggga
     6841 taaagcagac aggaatcggg cagggtgaaa agctgaagat gcaccgaacg gtaaagactg
     6901 cgcgcatcgc cttcctggaa atccgtgcgt ccgctgcctc ggatgagcag agcatcgccg
     6961 gtgaacgcca tgctctcatc gccgagcaca tagctgacgc agccccgcgt atggcccggc
     7021 gtgtcgcgta tctcga
//
LOCUS       DASYEG010000112         7499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_469359, whole genome shotgun sequence.
ACCESSION   DASYEG010000112 DASYEG010000000
VERSION     DASYEG010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 7499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7499
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469359"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VGM72_04500"
     CDS             complement(<1..429)
                     /locus_tag="VGM72_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEY4264554.1"
                     /translation="MKTARYGLRLSYTIILTLMLSVFAVVANAAEFTARATGVTDGDT
                     LTVLTGNNESLRIRLVEIDAPESDQPWGANAKQALSALVLGQSVRVVSTGQDEYGRTL
                     ARIFVNGKDVNAEMVRTGSAWAYRHYLTDTSLLAMERDAQN"
     gene            complement(567..1514)
                     /locus_tag="VGM72_04505"
     CDS             complement(567..1514)
                     /locus_tag="VGM72_04505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4264555.1"
                     /translation="MGAIAAKTIISWSASDGASHDGPLISRKNGYDVIACDVCGFRHV
                     VPLPDPDALERTYREAYYSEEKPTFLVHAGEDQEWAELAQRDRLESFERLLPPGQRRI
                     LDIGCGPGFFLKTAKERGWTVQGLEPSRQAAEHARGLGLDIVEEFFNATTAARLAGRY
                     DVVHLNNVLEHVPNPLTVVRLARDLLSRSGILCLNAPNDFTPFQESARASLSLPEWWV
                     APPHHLNYFDFESLSHVVATAGFRIAERGTSFPMEMFLLMGLDYTTNPELGLACHNQR
                     KRFDLGLEKAGFKETRRAFYRALAQSGIGREAVIIAVKN"
     gene            1582..2445
                     /locus_tag="VGM72_04510"
     CDS             1582..2445
                     /locus_tag="VGM72_04510"
                     /inference="COORDINATES: protein motif:HMM:NF015087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEY4264556.1"
                     /translation="MAVTFIAEASSNHAQDLSRALAFVDAAADCGCDAVKFQLFRLKE
                     LFAPEILAKSASHRARIAWELPVAHLAPLAARAKERGIAFSCTPFYLAAVAELEPYVA
                     FYKIASYELLWRGLLETCARTGKPVIISTGMATMPEIMTAVETLKDAGCRAITALHCV
                     SAYPAPAEDANLSAMQAIRESTGVAVGWSDHTRNPAVIERAVHRWDASAVEFHLDLDG
                     QGAEYASGHCWLPDEIAPVIARIREAERSDGTGFKEPAASELSDREWRADPHDGLRPL
                     KHIRAQWKPAA"
     gene            2442..4259
                     /locus_tag="VGM72_04515"
     CDS             2442..4259
                     /locus_tag="VGM72_04515"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEY4264557.1"
                     /translation="MSENAHRPRVVAIVQARMGSTRFPGKVLRPIAGKPLLWHVVHRL
                     RRAATLDAIVIATSTNPRDDAIRNFCREEKIDCVRGPEDDVLARFALAAARSNADVIV
                     RISSDAPFLDGPFIDHLVKALIAQDGDYVLMEPGALCAHEGVDPFSRRALDKLIREAP
                     DDRIAREHVSGYFKRHPDFVRIVYAAPFSTLERKSARLTIDTPDDLAFIETLHERLNV
                     KAGEAVLSDLLLLLEREPHLVQLNAHIRQKPLEAEGGLALIRCDGGGMLGFGHVKRCL
                     TLARALRDREGLGVLFVLNGEEDAAETIRQDGFETIVLPRNSQTTAFMALVTAKKPDI
                     LIADARTNLSRDMLMRIAQKVRIVAVIDDGTDRRLAATHAYYPPAPQVESLSWKGART
                     IVHSGWEWALLGFDPARLPVKPALRDRARPTIVVSMGGSDPLELTRLAARALAKVTAS
                     YRARFIIGPGFRGAPALAREIEAMSPAFEIVQGMNDLGAEFSGADLALVTFGVTAYEL
                     AALGVPALYLALSDDHALSASSFEKGGMGAVLGLGRIIRADDIARNCWQLLADEDRRR
                     DMRAAGLTTIDGRAGSRIAADLANALAEARAATPLALAQ"
     gene            4393..7194
                     /locus_tag="VGM72_04520"
     CDS             4393..7194
                     /locus_tag="VGM72_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264558.1"
                     /translation="MVVSFDPSLLMNYYMAKLPLASSQTFSSPSQQNKTQTQTPPWDI
                     SIAKPAQEIRDVTARNSDPYFDPKDALLSATATGSAATNAQSQIAALLNSTLSNTSTS
                     ATNAAMSADNDKMFALYSALDRLDYIAQMATRDGTVDGQLSGLNQSFQTGLNQVLSFV
                     QNTGLSNLTVLPGQTTSTAQSSVTIAYPQSDYTGGAAVQDKQILNPVPGVSASDSFTV
                     SITKAGVKSDVVIDLSQVSGPLTIDNINTYANQQLAAAGFATRLTRVQTGGSLTDATA
                     TWGEQIAYRPGETVSLSSNQAKPSVYVAGISGLSSDSDGKLIKLAGLDGTPSSAFSTN
                     IAPTSGTATAKNTVVDANGNVYVIGDSTGSFGNEVNQGAQDVYLTKYDSAGNQQWTKL
                     LGSADSASGYGLAVDPKSGGVVIAGAVTGDLTPTAIGSGTDSFVAKYDSSGNQTWLRQ
                     VAPVNNDQANSVSVDADGNIYVGGQVNGAIASGATSAGGTDAYVTKLDSTGKLVYQRQ
                     FGTAGADSAAQTAVAPDGNVIVASVQNGHAILSKYASADGTSAAMWQIDLGDLKGGSI
                     GGLTVSGGKVYVSGTSSNAALDAGGTASIANANSGGADGFVFAATDAGASASADFVSY
                     VGTGATDQGGGVAVAGGNIYLTGTTSGTFAGQVRNSANTHNMFVAQLGADGTLNWTQQ
                     YGGLDGESEGLAIAADNSGSSVLDALKLQRGTISVNQSNAIESQTTARAGDYFTLQIK
                     DATGSRSAKITIAKGETLRSLAIKINSALQSDGKATASAAVGGQGLKIAVNPGAQIQL
                     VSGPKDFDALAGLGLKPQLLTNVAATSTPSTSSSTKVTDNAAVKPAPTTTPATTGPQI
                     IGLGINGGIDLLSKDTAAHANVVLQGAMSLIKQAYSTLNTPQTSTATAATGSVPAYVQ
                     SQLASYQTALAYMQSLTTS"
     gene            complement(7301..>7499)
                     /locus_tag="VGM72_04525"
     CDS             complement(7301..>7499)
                     /locus_tag="VGM72_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4264559.1"
                     /translation="VSTKGHTLDHIGFDVKNLEAFCKKAAADGIKVDRPYTKNAGGTG
                     ICFIYDPWGTYIELNEREKPL"
BASE COUNT         1473 a         2358 c         2266 g         1402 t
ORIGIN      
        1 gttttgcgcg tctctctcca ttgcgagaag cgatgtatcc gtcagatagt ggcgataggc
       61 ccacgccgag cctgtgcgca ccatttcggc attcacgtct ttgccgttca cgaagatgcg
      121 cgcgagcgtt cgcccgtact catcctgccc tgtagagacg actcgcaccg attggcccaa
      181 aacgagcgcc gacaatgctt gtttcgcatt tgcgccccag ggttgatcgc tttccggcgc
      241 atcgatttcc acaaggcgaa tccgcagcga ctcattgttg ccggtaagaa ccgtcagcgt
      301 gtcgccgtcc gtgactcccg tagctcgtgc cgtaaattca gcggcgttcg caacaacagc
      361 gaagactgac agcatcagtg tcagaatgat cgtataggat agcctaagtc catatcgggc
      421 cgtcttcatc cgatcatcct tcaagagttc ttgcgggaaa aacgctcgga cgcgggaaaa
      481 aggtagtaag acgttcgctg tgaataaaat cttacagaca gacgttgaag atcttgctgc
      541 aaggcttcgc gcggcagaga tctgcgttaa ttcttcacgg cgatgatgac ggcctcgcgt
      601 ccgattccgg actgggcgag cgcgcgatag aaggcgcggc gggtctcctt gaagccggcc
      661 ttttccaatc caagatcgaa gcgctttctc tgattgtggc aggcgagccc gagctccggg
      721 tttgtcgtgt agtcgagtcc catcagcagg aacatctcca tcgggaagct ggtgccacgt
      781 tcggcgatgc gaaaacccgc ggttgcaacg acatgggaga gggattcgaa gtcgaaataa
      841 ttcagatgat gcgggggggc gacccaccat tcggggagcg aaagcgatgc ccgcgcgctc
      901 tcctgaaagg gggtgaaatc gttcggcgcg ttgagacaga gaattccgga acggctgagc
      961 aaatcgcggg cgagccgcac cacggtcaag ggattgggca catgctccag cacattgttc
     1021 agatgcacca catcgtaacg cccggctagg cgcgccgccg tcgttgcgtt gaagaattcc
     1081 tcgacgatgt cgaggccgag gccacgcgca tgctcggctg cctgacgtga aggctcgagc
     1141 ccttgcaccg tccagccgcg ctccttggcg gttttgagga agaatccggg tccgcagccg
     1201 atatccagga tgcggcgctg ccccggtggc aaaagccgtt cgaagctctc cagccggtcg
     1261 cgctgggcca attccgccca ttcctgatct tcaccggcat ggacgagaaa cgtaggtttt
     1321 tcttcgctgt aataggcctc gcgataggtg cgttcgagcg catccggatc gggcagcggc
     1381 acgacatggc gaaagccgca cacgtcgcag gcaatgacgt catagccgtt tttgcggctg
     1441 atcagaggcc cgtcgtggct cgcgccgtcc gatgcgctcc aggagatgat ggttttggct
     1501 gcgatcgcgc ccatttggtc cttccgttaa ctaaggttta gccatgtctc tttaaaaagc
     1561 cgttaagcgg gaggacaaac tgtggctgtt accttcattg ccgaagcatc cagcaatcac
     1621 gctcaagacc tttcgcgcgc gctcgccttc gtggatgcgg cggcggattg cggctgcgat
     1681 gcggtgaaat ttcaactgtt ccggctgaag gaattgttcg cgcccgagat cctcgcgaaa
     1741 agcgcatcgc atcgcgcgcg tatcgcgtgg gagctgccgg tcgcccatct tgcgccgctt
     1801 gccgcgcggg caaaggaacg cgggatcgcg ttctcctgca cgccgtttta tctcgccgcc
     1861 gtcgccgaac tcgaacccta tgtcgccttc tacaagatcg cgtcctatga gcttttgtgg
     1921 cgcggcctcc tggagacatg cgcgcggacc ggcaagccgg tcatcatctc gacgggcatg
     1981 gcgaccatgc ccgaaatcat gacggcggtg gaaacgctca aagatgcggg ctgccgcgcg
     2041 atcaccgcgc tgcattgcgt ttcggcctat cctgcgccag ccgaagacgc caatctttcc
     2101 gcgatgcaag cgattcgcga atcgacaggc gttgcggtcg gctggtccga ccacacgcgc
     2161 aaccccgccg tgatcgaacg cgcggtccat cgctgggacg cgtcagcggt ggaattccat
     2221 ctcgacctcg acggtcaggg cgccgaatac gcttcaggcc attgctggct tcccgatgag
     2281 atcgcacccg taatcgcgcg catccgcgaa gccgaacgca gcgatggcac cggcttcaag
     2341 gaaccggcgg cctcggagct ttccgaccgc gaatggcgcg ccgatcccca tgacggtctg
     2401 cgtccgctca agcatatccg cgcgcaatgg aagccggcgg catgagcgaa aacgcacatc
     2461 gcccgcgcgt cgtcgccatc gtacaggccc gcatgggctc gacccgcttt cccggcaagg
     2521 tcctcagacc catcgcggga aagcccttgc tttggcatgt ggtgcatcgc ctgcgccgcg
     2581 ccgcgacact cgatgcgatt gtgatcgcca cctcgaccaa tccgcgcgac gacgcaatcc
     2641 gcaatttctg ccgcgaagaa aaaatcgatt gcgtgcgggg gcccgaagac gacgtgcttg
     2701 cgcgtttcgc cctggccgcc gcgcgctcca acgccgatgt gatcgtgcgg atttcgtccg
     2761 atgctccgtt cctcgacggg ccgttcatcg accacctggt gaaagctctg atcgcgcagg
     2821 acggcgatta tgtgctgatg gaacccggcg cgctttgcgc ccacgaaggc gtggacccat
     2881 tctcccgccg cgcgctggac aagctcatcc gcgaggcgcc ggacgaccgc atcgcccgcg
     2941 aacacgtcag tggctatttc aaacggcatc ccgatttcgt gcgcatcgtt tatgccgccc
     3001 ccttttcgac gctggagcgc aaatccgcgc gactcaccat cgatacgccg gacgatcttg
     3061 ccttcatcga aacattgcac gaacgcctta acgtcaaagc tggtgaggct gtcttgtcag
     3121 atcttctgct cctgctcgaa cgcgaacccc atctcgtcca actgaatgcg catatccgcc
     3181 agaagccgct cgaggccgaa ggcggactcg cgctgatacg ctgcgacggc ggcggcatgc
     3241 tgggattcgg ccatgtgaag cgttgcctga cgctcgcgcg ggccttgcgc gaccgcgagg
     3301 gcctgggcgt gctgtttgtc ctcaacgggg aggaggacgc cgccgaaacc atccgtcagg
     3361 acggatttga aaccattgtg ctgccgcgca acagccagac gacggccttc atggcgctgg
     3421 tgacggcaaa gaagccggac atcctgatcg cggacgcgcg caccaacctg tcgcgcgaca
     3481 tgctgatgcg gatcgctcag aaggtgagaa ttgtcgccgt gatcgatgac gggaccgacc
     3541 gccgcctggc ggcaacgcac gcctactacc cgccggcgcc ccaggtcgaa agcctgtcgt
     3601 ggaaaggcgc ccgcaccatt gtgcacagcg gatgggaatg ggcgcttctc ggcttcgacc
     3661 cggcgcggct gccggttaaa ccggcactgc gcgatcgcgc ccgccccacc atcgtcgtca
     3721 gcatgggcgg gagcgatccc ctggaactga cgcgcttggc cgcgcgcgca ctcgccaagg
     3781 tcaccgcttc ttatcgcgcg cgcttcatca tcggcccggg ctttcgcggg gccccggccc
     3841 tcgcacgtga gatcgaagcg atgagcccgg cctttgaaat cgtccagggc atgaacgatc
     3901 tgggcgcgga attttccggc gccgatcttg cgctcgttac ctttggcgtc accgcctacg
     3961 aactcgccgc cctcggggtg ccggcgcttt atctcgcgct ctccgacgat catgcgcttt
     4021 cggcttcgag cttcgagaaa ggcggcatgg gcgcggtgct gggcttggga agaatcatcc
     4081 gtgccgacga tatcgcacgc aactgctggc aattgcttgc cgatgaagac cgccgccgcg
     4141 atatgcgcgc ggccggtctg accacgatcg acggccgcgc cggctcgcgc atcgcggccg
     4201 atctggcgaa tgctctggcc gaggcccgcg ccgccacgcc gctggcgctg gcgcaataag
     4261 gcatcggggt tcgaggccgc ttatatcagg gtaacgcacg cttaaccgta ccggctctta
     4321 gtgtgctgtt aaggaaagtc cgcgcactct tctctggaag ggtgcaaagc gttttgcgca
     4381 ggaagacata tcgtggtggt cagtttcgat ccttctctgc tgatgaatta ttacatggcg
     4441 aagctgccgc tcgcctcgtc gcagacattt tcgtctccct cgcagcagaa taaaactcag
     4501 acccagacac cgccctggga catcagcatc gccaagcccg cgcaagagat tcgcgacgtc
     4561 accgcgcgca acagcgaccc ctatttcgat cccaaagacg cgcttctgtc cgcgacggcg
     4621 accggaagcg cggcgacaaa tgcgcaatcg cagatcgcag cacttctgaa cagcacgctg
     4681 tccaacacat cgacgtctgc cacgaatgct gcgatgtcgg cggacaacga caagatgttc
     4741 gcgctctaca gcgcgctcga ccgtctcgac tacatcgcgc aaatggccac acgcgacggc
     4801 acggtcgacg ggcaactttc cggtctgaac cagagcttcc agacgggtct caatcaggtg
     4861 ctttctttcg tgcagaatac gggtctgtcg aatctgaccg tgcttccggg gcagacgacg
     4921 tccaccgcgc agagctccgt caccatcgcc tatccgcaat cggattacac cgggggcgcg
     4981 gcggtccaag acaagcagat cctgaatcct gttccgggcg tctcggcgag tgacagcttc
     5041 acggtgtcca tcaccaaggc aggggtgaag agcgatgtgg tcatcgatct ttcgcaggtc
     5101 tcaggtccgc tcaccatcga caacatcaac acctacgcca atcagcagct cgcggcggcc
     5161 ggctttgcca cgcggctcac ccgcgtgcag accggcggct cgctcacaga cgccacggcg
     5221 acatggggcg agcagatcgc ctatcggccg ggcgagacag tcagcctgtc atccaatcag
     5281 gcaaagccat cggtttatgt cgcgggaatc agcggtcttt cgagcgacag cgacggcaag
     5341 ctgatcaagc tcgcgggcct tgacgggacg ccatccagcg ccttttccac gaacattgcg
     5401 cccacgtccg gcacggccac cgccaagaac accgtcgtcg atgccaatgg caatgtctat
     5461 gtgatcggag attcgaccgg cagcttcgga aacgaagtca atcagggcgc gcaggacgtc
     5521 tatctcacca aatatgattc cgccggcaat cagcaatgga ccaagttgct gggttcggcg
     5581 gattcggcca gcggctacgg actcgccgtc gatcccaaat cgggcggcgt ggtgatcgcg
     5641 ggtgcggtga cgggcgatct tacgccgacg gcgatcggta gcggcaccga cagtttcgtc
     5701 gcgaaatatg acagctccgg caaccagacc tggctacgcc aggtggcgcc ggtgaacaat
     5761 gatcaggcca acagcgtgag cgtggacgcg gacggcaaca tctatgtcgg cggacaggtg
     5821 aacggcgcta tcgcatcggg cgcgacgtcg gcgggcggca cggacgctta tgtgacgaag
     5881 ctcgatagca ccggcaagct cgtctatcag cggcagtttg gcaccgccgg cgcggattcc
     5941 gctgctcaga cggccgtggc gccggacggc aatgtgatcg tggcgagcgt ccagaacggc
     6001 cacgcgatcc tttcgaaata tgcgtctgcg gacggcacga gtgctgcgat gtggcagatc
     6061 gatctcggcg atctgaaagg cggatccatc ggcggcctca cggtttccgg cggcaaggtc
     6121 tatgtctcgg gcacatcctc gaatgccgct ctcgatgccg gaggaaccgc atccatcgcc
     6181 aatgccaatt cgggcggcgc ggacggtttt gtcttcgcgg caaccgacgc gggggcaagc
     6241 gcgagcgcgg atttcgtctc ctatgtaggg acgggggcaa ccgatcaagg cggcggcgtc
     6301 gcggtggcgg ggggaaatat ctatctcacc ggcacgacca gcggcacctt tgcgggccag
     6361 gtccgcaata gcgcgaacac acacaatatg ttcgtggccc agctcggagc ggacggcacg
     6421 ctgaattgga cgcagcaata tggcggcttg gacggcgaat cggagggatt ggcgatcgct
     6481 gccgacaatt cgggctccag cgtgctcgat gcgctcaaac ttcaacgcgg gaccatctcc
     6541 gtcaaccaat ccaacgccat cgaatcgcag accaccgcgc gcgcgggcga ttatttcacg
     6601 ctccagatca aggacgcgac cggatcgcgc agcgcgaaaa tcacgatcgc caagggcgag
     6661 accttgagat cgctggccat caagatcaac agcgcgttgc agtccgacgg caaggcgacc
     6721 gcttcggccg ccgttggcgg gcagggtttg aagatcgcgg tgaatccggg cgcacagatc
     6781 caactcgtct cgggacccaa ggatttcgat gcgctcgctg ggctgggact gaagccgcaa
     6841 ttgctgacca atgtcgctgc gaccagtacg ccaagcacat cgtcgagcac caaagtgacc
     6901 gacaatgcag cggtcaaacc cgcgcccacg acgacacccg caacgacggg gccgcaaatc
     6961 atcgggctcg gtatcaatgg cggcatcgat cttttgagca aagacaccgc cgcgcacgcc
     7021 aatgtcgtcc tgcaaggcgc gatgtcgctg atcaagcagg cctattcgac gctgaatact
     7081 ccgcaaacat cgaccgccac ggccgcgacg ggcagcgtgc cggcctatgt gcaaagccaa
     7141 ctcgcaagct atcagaccgc gctcgcctat atgcagtcgc tgaccacgtc gtgactgcgc
     7201 ctaaagcgtg attgatttgg attgaatcgg aaatcacgct ctaggttttg tttgtcgcat
     7261 tttttcaggc tgaaccggca tccacttcag ccaaaatgct ctaaagcggt ttttcgcgct
     7321 cgttcaattc gatataggtg ccccagggat cgtagatgaa gcagatcccg gtcccgcccg
     7381 cattcttggt ataggggcgg tccactttga ttccgtcagc cgccgccttc ttgcagaagg
     7441 cttccagatt cttcacgtcg aaaccgatgt gatccagcgt gtggcccttg gtgctgacg
//
LOCUS       DASYEG010000113         3630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2065751, whole genome shotgun sequence.
ACCESSION   DASYEG010000113 DASYEG010000000
VERSION     DASYEG010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2065751"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VGM72_04530"
     CDS             <1..247
                     /locus_tag="VGM72_04530"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY4264560.1"
                     /translation="VAEAYPQLRAAENFQSLQKDLAEIEDQIQLARRYYNGAVRDYNV
                     TTEQFPSNLIASSFGFTKAPFFGIENAADRDVPRIAF"
     gene            244..2166
                     /locus_tag="VGM72_04535"
     CDS             244..2166
                     /locus_tag="VGM72_04535"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEY4264561.1"
                     /translation="MILFARAFLFVAIAFASVSAAVAEERITDFASDIAIARDGTLSV
                     RETISVNAEGDRIQHGIFRDFPTLYRDAAGNRLRVRFDVAEVDLDGHPEPYTLETISN
                     GTRVRIGEANVVLNPGAHTFLIVYRTDRQIGFFQDYDELYWNVTGNGWDFPISRAEAV
                     IHLPPGAHLKQYAFYTGPQGAAGKDAQAFPEGDSVRFVTNASLNPREGLTVAAGFDKG
                     AVIPPSRADRAGNFLRDNASAGAALLGLIALAIYYAIAWSRFGRDPARGVIVPLFAPP
                     QDFSPAAVRFVYRMGYDRKAFAAALIAMAVKGYLKITESHGVYTLTRTGVSQAASGLA
                     STEGAIANVLFNIANEITLKNTNHTIVARAITALRDALRKEDEGVYFVTNRGWFLGGV
                     AILVLSGAAAALLSEDAAPSAFILIWLAVWTAGTSHLLLNAYQAWRGVIAGPGSRVLN
                     FFGAAVLTLFAAPFVAGLVFGIYFLGLSFPIAPLIAILAQGVLAAVFYRLLKAPTLAG
                     AKIRDSIDGFKMFLDVAEKDRLEVLHPPQVTPEVFEKFLPYAIALDAENAWSRKFEMQ
                     AAAAGAGANQGGYVPVWYSGPSFNRLGTTGFASSLGSAMAGATAEAATAPGSSSGSSG
                     GGSSGGGGGGGGGGGW"
     gene            complement(2201..2632)
                     /locus_tag="VGM72_04540"
     CDS             complement(2201..2632)
                     /locus_tag="VGM72_04540"
                     /inference="COORDINATES: protein motif:HMM:NF014144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HEY4264562.1"
                     /translation="MKRRGSLLRLKRFRVDELKRRLATLDEMKADLEKKLTDLEDSVV
                     RERQRASDSDIGRLAFPSFLQSIEVRRKNIRTTMADLERERAAQQDDLTAAFQDLKSF
                     ELAEEERLRRASEAEARAQQSRMDEIAMTRHLRKHAARQTG"
     gene            complement(2629..>3630)
                     /locus_tag="VGM72_04545"
     CDS             complement(2629..>3630)
                     /locus_tag="VGM72_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0046034 - ATP metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HEY4264563.1"
                     /translation="PLVCGMEAYPLRAAPPSAHARARLGGKLDLGVRALNAFTTCCKG
                     QRMGIFAGSGVGKSILLAMLARNTAADVTVIALVGERGRELKEFIEDDLGPEGLARSV
                     VVAATSDEAPLTRRQAAFVAMATAEYFRAQGLHVLLLMDSVTRFAMAQREIGLSAGEP
                     PASKGYTPTVFAELPRLLERAGPGPSGTTGSITGLFTVLVEGDDHNEPISDAVRGILD
                     GHIVLDRAIAERGRYPAINVLRSVSRAMPQCNAPAEQATIIQARGQLAIYEDMAELIR
                     LGAYRTGTNPEVDRAIALYPHLEAFLSQQKNERTPMAAGYANLSKILDAMGTKGSGR"
BASE COUNT          625 a         1163 c         1129 g          713 t
ORIGIN      
        1 ggtggccgaa gcctatccgc aattgcgcgc tgctgagaat ttccaatcgc tgcagaagga
       61 cctggccgag atcgaagatc agatccagtt ggcgcggcgc tattacaatg gcgcggtccg
      121 cgattacaat gtgacgaccg aacaattccc ctcgaacctc atcgcatcgt ctttcggttt
      181 caccaaagcg ccgttcttcg gaatcgagaa tgccgcggac cgggacgtgc ccaggatcgc
      241 gttttgattc tgttcgcccg cgcttttctt tttgtcgcga ttgcgtttgc atccgtatcg
      301 gctgccgtcg ccgaggagcg catcaccgat ttcgccagcg acatcgccat cgcccgcgac
      361 ggcacgctca gcgtgcgcga gacaatcagc gtgaatgccg aaggcgaccg gatccagcac
      421 ggtattttcc gtgatttccc gacgctctat cgtgacgccg cgggaaatcg gctgcgtgtg
      481 cgcttcgacg tcgcggaggt cgatcttgac ggccatcccg aaccgtacac gctcgaaacc
      541 atctccaacg gaacccgggt gcgcatcgga gaggcgaatg tcgtcctgaa ccccggcgcg
      601 catacattcc tgatcgtgta tcgcaccgac cggcagatcg gattcttcca ggattatgat
      661 gaactgtact ggaacgtcac cggcaatggc tgggacttcc cgatcagccg cgccgaagcc
      721 gtgatccatc tgccgccggg cgcgcatctg aagcaatatg cgttctacac cggaccgcaa
      781 ggtgccgccg gcaaggacgc gcaagctttt ccggaaggcg acagcgttcg tttcgtgacc
      841 aacgcgtcgc tgaatccccg cgaggggctg accgtcgcgg ccgggttcga caagggcgcc
      901 gtgattcctc catcccgcgc ggaccgcgcc ggaaatttcc tgcgcgacaa tgcctccgcc
      961 ggggccgcgc tcctcgggct gatcgcgctc gcgatctatt acgcgatcgc atggtctcgc
     1021 ttcggacgcg atccggcacg cggcgtgatc gtccccctct tcgcgccgcc ccaggacttc
     1081 tcccccgccg cggtgcgctt cgtgtatcgc atgggttatg accgcaaggc attcgccgcc
     1141 gcactgatcg ccatggcggt gaagggctat ctgaagatca ccgaatccca tggcgtttac
     1201 accctcacgc gcacgggcgt cagccaggcc gcgagcgggc tcgcctccac cgaaggcgcg
     1261 atcgcgaacg tcctcttcaa catcgccaat gagatcacgc tcaagaatac caaccacacc
     1321 atcgtcgcgc gcgcgatcac ggccttgcgc gacgcgctca ggaaagaaga cgaaggcgtc
     1381 tatttcgtca ccaatcgcgg ctggtttctg ggcggcgtcg cgatccttgt cctcagcggc
     1441 gcggctgcgg cgttgctctc cgaggacgcc gcgccttccg cgttcatcct gatctggctt
     1501 gccgtgtgga cggcgggcac gtcgcatctt ttgttgaatg cctatcaggc ttggcgcggg
     1561 gtcatcgccg gccccggctc gcgcgtgctg aatttcttcg gcgctgccgt tctcacccta
     1621 ttcgcggcgc ctttcgtcgc gggattggtt ttcgggattt attttctcgg tctgtctttc
     1681 ccgatcgcgc cgctgatcgc cattttggcg caaggggtgc tggcggctgt tttctaccgc
     1741 ctcctgaagg cgccgaccct ggccggcgcg aaaatccgcg attccatcga cggcttcaag
     1801 atgttcctcg atgtcgcgga gaaagaccgc ctcgaagtct tgcacccgcc gcaggtgacg
     1861 ccggaggtgt tcgaaaaatt ccttccctat gcgatcgcgc tcgatgccga aaatgcttgg
     1921 agccgcaaat tcgaaatgca ggcggcggcg gcgggcgcag gcgccaatca agggggctat
     1981 gtgccggtct ggtactcggg cccgtcgttc aaccggctcg gcaccacggg atttgcgtcg
     2041 tcattgggaa gcgcgatggc cggcgcgacc gcggaggcgg caacggcacc gggcagctcg
     2101 tcgggttcgt ccggcggggg atcgtccggc ggcggcggtg gaggcggtgg cggcggcggc
     2161 tggtgagacc aaaggtcgcg ccgtcaggtc cctgaccgaa tcaaccggtc tggcgcgcgg
     2221 cgtgctttct cagatggcgc gtcatcgcga tttcgtccat gcgcgactgt tgggcgcgcg
     2281 cttcggcctc cgacgcgcgg cgcagacgtt cttcctcggc caattcaaaa ctcttcagat
     2341 cctgaaaggc ggccgtgaga tcgtcctgct gagcggcgcg ttcgcgctcc agatccgcca
     2401 tcgtggtgcg gatatttttc cggcggactt cgatggattg caggaaactt gggaacgcca
     2461 gccggccgat atccgaatcg ctcgcgcgct gacgttcgcg cacgaccgaa tcttccagat
     2521 cggtgagctt cttctccaga tcggctttca tctcgtccaa tgtggcgagc cgtcgcttca
     2581 attcgtcgac gcgaaagcgc ttcaagcgca aaagggaacc acggcgtttc atcggccact
     2641 acctttcgtt cccatagcat ctaggatttt gcttaaattc gcgtaaccag ccgccatcgg
     2701 cgtgcgctcg ttcttttgct gcgagagaaa cgcttcgaga tgcggataaa gcgcgatggc
     2761 acgatccact tcggggttcg ttcctgtgcg gtaggccccg agccgaatca gttcggccat
     2821 gtcctcatag attgcgagct ggccgcgcgc ttggatgatg gtggcctgct cggcgggcgc
     2881 attgcattgc ggcatggccc gcgacaccga tcggagcacg ttaatggcgg gatagcgtcc
     2941 gcgctcggcg atggcgcggt cgagcacgat gtgaccgtcg agaatgccgc gcaccgcatc
     3001 tgaaatcggc tcattgtggt cgtcgccctc gaccagaacg gtgaacagcc cggtaatgct
     3061 tccggtggtg ccggaagggc ctggccccgc gcgttcgaga agccggggca attcggcgaa
     3121 tacggtcggc gtataaccct tggaggcggg cggctcaccc gccgacaacc caatttcgcg
     3181 ctgcgccatg gcaaagcggg tgaccgaatc catcaacaga agaacgtgca gaccctgggc
     3241 acggaaatat tccgcggtcg ccatggcgac gaacgccgcc tgacgccggg tcaagggcgc
     3301 ttcgtcggat gtcgcggcga cgaccacgct cctggccagt ccttccggcc cgagatcgtc
     3361 ttcaatgaat tccttcaatt cgcggccgcg ctcgcccact agtgcgatca ccgtgacgtc
     3421 ggccgcggta ttgcgcgcca gcatggcgag cagaatggat ttgccgacgc cggagccggc
     3481 gaaaatgccc atgcgctggc ctttgcagca ggtcgtaaag gcattgagcg ctcgcacacc
     3541 cagatcgagt ttgccgccga gacgcgcgcg tgcatgggcc gaaggcggcg cggcacgcag
     3601 cggataagcc tccatcccgc aaaccagcgg
//
LOCUS       DASYEG010000114         6114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1048745, whole genome shotgun sequence.
ACCESSION   DASYEG010000114 DASYEG010000000
VERSION     DASYEG010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048745"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VGM72_04550"
     CDS             complement(<1..285)
                     /locus_tag="VGM72_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264564.1"
                     /translation="MLNAVSPRQQEGSLDEALARATAPVSEILDRALNGNELGFSDGI
                     LLGDVAGDDLIALVKTADEMRRRKIGETITYVVNRNINFTNICFVGCAFCG"
     gene            complement(279..1493)
                     /gene="cofG"
                     /locus_tag="VGM72_04555"
     CDS             complement(279..1493)
                     /gene="cofG"
                     /locus_tag="VGM72_04555"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF004884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HEY4264565.1"
                     /translation="MSLAFATAGFAKNPESREIARALARGEDGNGLSRAEALLLLEQA
                     PSDALIDAASRLRDRVKGRIVSYSKKVFIPLTHLCRDYCGYCTFRADPKDGHPAYMSP
                     DEVLALADAGRRAGCKEALFSLGDQPERIFPEAREFLNRLGFTTTLDYLAAMSRAVLE
                     KTGLLPHSNPGLMGADDLARLRETNVSLGLMLESISSRLRHKGAAHWRAPDKAPLLRL
                     RTIEDAGRQKIAFTTGILIGIGETREERVDSLLAIRCLHEQYGHIQEVLIQPFRAKTD
                     TRMAGASEPDMEDLQRTIAVARLILGDMNVQSPPNLVSEDYPRLLAAGINDWGGISPV
                     TRDFINPESAWPGIAVLDRRTKEAGFVLRERLAIYPEFARDDAFVDPALRPQLDSHRD
                     ADFYARAGSLEC"
     gene            complement(1501..2307)
                     /locus_tag="VGM72_04560"
     CDS             complement(1501..2307)
                     /locus_tag="VGM72_04560"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY4264566.1"
                     /translation="MSEDVSGEMIQCPGGMPGFLAHPVTPGRYPVVILMHERYGLVSH
                     TKDLASRCARDGFTVLAPNFFARHPDSAALNAGAARYDMSDPESVSLLTSALAALENH
                     PFADMSKIAIAGYCQTGRHPLVFAAQTKIQAAIVWYGAASKREWGVTDIQPKALDVIV
                     ARIDCPVFGAFGSDDHIISVDDVRRFRDALETHKKSYDIRLYAAAPHGWLNDTMPGRY
                     RKAQADAALAAQQQFLKEVFAGEWPGTSIRWRFDSSFGEDYDFANNRRQE"
     gene            complement(2316..2963)
                     /locus_tag="VGM72_04565"
     CDS             complement(2316..2963)
                     /locus_tag="VGM72_04565"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEY4264567.1"
                     /translation="MGRITRLEKELSAARTGLVLFDTLNGYLHPRDNPAKLRFLAERN
                     ILGHMQRLLAGARKSGMIVFYPSGAHDPTGADSVDRLTDTDMELAPGGNAAAPIRPHF
                     HKGSVDADIAPELAPGPRDVVVPKQRWSSFFQTNFDLQLRTRGIDTIVIAGGSTDVGI
                     ASTVFAARDLDYGIVVVRDCCYSTRGNNNDFFMDRVFPRMARVMDADQAVALMTA"
     gene            complement(2976..4004)
                     /locus_tag="VGM72_04570"
     CDS             complement(2976..4004)
                     /locus_tag="VGM72_04570"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY4264568.1"
                     /translation="MRNLSRHMALALAASLLWTGVARAQSNDGFYKGKTVTLVVSSSA
                     GGGYDIMGRTIAKYLGSHIPGNPRVVVSNMPGAGGIVAMNYFYRNAPKDGTFIAGMQN
                     NTPFEPLLGTKEAQYDATKFNWLGSPSVEVGLIAVWRNVPVNSIADLKKREITVGSSG
                     ANSTPSFYARLINATLGTKMKIVVGYPGQNEVYFAMERGEVDGFPSLFYNTLNATRPN
                     WRAEKNIKLILQYGLEKEPAIPEVPSALDLAANADDRQLLKAGLAEVSLGRPYLMPPG
                     VAPARVALMRKALADTFADKGFLADSKRMALGVNTPKSGAQIQQLIADTYRISPKTIE
                     RLRKLSLH"
     gene            complement(4027..4890)
                     /locus_tag="VGM72_04575"
     CDS             complement(4027..4890)
                     /locus_tag="VGM72_04575"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY4264569.1"
                     /translation="MDVLTKTRLDIVPLSQHIGAEIRGMDLRERPDNETIHAIYQAWL
                     DHLVLVFPGQTLSQEDLLRVTTYFGENGHLDRPAKYHPPGFSRMLPGIMLISNIRENG
                     EPIGALPDGEMMFHHDMIHKEVPSKATLLYSVEIPSYGGNTLFASGYAAYETLDPAIR
                     ERLEGRLALHHYNYGSTQRGDDRGTEAFNECVHPIFRTHEDTGRKAVYVNRLMTVKIL
                     DMEPDESMRLLGALFDQAERTEFVYSHRWRKDDLLLWDNRCSSHAREDFPSTERRLML
                     RTTVKGTVRPS"
     gene            complement(4962..>6114)
                     /locus_tag="VGM72_04580"
     CDS             complement(4962..>6114)
                     /locus_tag="VGM72_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEY4264570.1"
                     /translation="TDPLFDAWIEAAKAAGFPHVQDYNAGSHEGFGRSQYSIRNGRRS
                     SSASAYLRPARKRPNLTVHTGALTTRILTQGTRAIGVEYLQGGQTIRANADAEVIVSA
                     GTFNTPQILMLSGIGPAEHLREIGIAPLVDLPVGKNLQDHVAALITYSRPNPGPFREA
                     MRFDRMARGMVEAHFRGTGIATVVPGGLHAFVKTRPGLAVPDIEFMFRGAPTNAALWF
                     PLVKPAYADGFGIRPTLLHPQSRGAVKLASADPRSPPRIFLNLLTVASDLGTLREGFK
                     RARDVAMQAPLDPYRGEEKSPGPKVQTDAEIDSWLKRVVVTAHHPASTCPMGTREDCV
                     LDPELRVRGMEGLRVVDASAMPDLVSAHINATVLMMAEKAADLIRARAA"
BASE COUNT         1161 a         1858 c         1929 g         1166 t
ORIGIN      
        1 gccgcaaaag gcgcagccga caaaacagat attggtgaaa ttgatattgc ggttcaccac
       61 ataggtgatg gtctcgccga ttttgcggcg gcgcatttca tcggcggttt tcaccagcgc
      121 gataaggtca tcgcctgcaa catcgcccaa caggatgccg tcggagaacc caagctcatt
      181 cccgttgagc gcgcgatcca gaatttccga cacgggcgcg gtggcgcgcg cgagcgcctc
      241 gtcgagcgaa ccctcctgct ggcgcggaga aacggcgttc agcattcaag gctcccagcc
      301 cgcgcataga aatccgcgtc ccgatgcgaa tccaattgcg gtctcaatgc cgggtccaca
      361 aaagcgtcat cgcgcgcgaa ttcgggatag atggcgagcc gctcgcgcaa gacaaagccg
      421 gcctctttcg tgcggcgatc cagaaccgcg attcccggcc aggcgctttc cggattgatg
      481 aagtcgcgcg tcacgggcga aatcccgccc caatcattga tccccgccgc aagcagccgc
      541 ggataatcct ccgacaccag attgggtggg gattgcacat tcatgtcgcc aaggatcagc
      601 cgcgccacgg cgatggtgcg ctgcaaatcc tccatgtccg gttcggatgc accggccatg
      661 cgcgtatcgg tcttggcgcg gaaaggttga atcaggactt cctggatgtg gccatattgc
      721 tcgtgcaggc agcggatggc gagaagcgaa tccacgcgtt cctctcgcgt ctccccgatg
      781 ccgatgagga tccccgtcgt gaacgcgatt ttctggcggc ccgcgtcctc gatggtgcgc
      841 aagcgcaaaa gcggcgcctt gtcgggagcg cgccaatgcg cggccccttt gtggcgcagc
      901 cgtgagctta tcgattccag catcaggccg aggctcacat tcgtctcgcg caaacgcgca
      961 agatcgtccg cccccatcag accgggattg gaatgcggca gtagcccggt tttttccagc
     1021 acggcccgcg acatcgccgc cagataatcg agcgtcgtgg taaagccgag ccggttcagg
     1081 aattcgcggg cttcggggaa aatccgctcc ggctggtcgc ccagactgaa cagcgcttcc
     1141 ttgcaccccg cgcgccgccc cgcatccgcg agcgccagca cttcgtccgg actcatatag
     1201 gcggggtgcc cgtctttcgg atcggcgcgg aaggtgcagt agccgcaata atcgcggcag
     1261 agatgggtca gcggaatgaa gactttcttc gaatagctga cgatccggcc cttcacgcgg
     1321 tcgcgcaggc gcgatgcggc gtcgatcaac gcatcgctcg gcgcctgttc cagcaagagc
     1381 aacgcctcag cgcgtgacag cccgttgccg tcttcgccgc gtgccagcgc gcgagcgatt
     1441 tctcggcttt ctggattttt cgcgaaaccc gctgtggcga aggctaggct catcgattgg
     1501 ctattcctgc cggcgattgt tggcgaaatc gtagtcctcg ccgaagctgc tgtcgaaccg
     1561 ccagcgtatg gacgtgccgg gccattcgcc cgcaaagacc tccttgagga attgctgctg
     1621 cgcggccaag gcggcgtctg cttgggcttt gcggtagcgc cccggcatgg tgtcgttcag
     1681 ccagccatgc ggcgccgcgg cataaagacg gatgtcatag gatttcttgt gggtttccag
     1741 cgcgtcacga aagcggcgca catcgtccac cgagataatg tggtcatcgg aaccgaacgc
     1801 gccgaagacc gggcaatcga tccgcgcgac gatgacgtcc agcgctttgg gctggatgtc
     1861 cgtcaccccc cattcgcgtt tggacgccgc gccataccag acgatggcgg cctgaatctt
     1921 cgtttgcgcg gcgaagacga gggggtggcg tccggtctgg caatagcccg caatcgcgat
     1981 cttgctcata tcggcaaagg gatgattttc cagggcggcc agcgccgatg tcagaaggct
     2041 caccgattcg ggatcgctca tgtcatagcg cgccgcgccc gcgttcagcg cggcggaatc
     2101 gggatgacgc gcgaagaaat tcggcgccag gacggtgaaa ccatcacgcg cgcaacgcga
     2161 cgccagatcc ttggtgtggc tcaccagacc gtagcgctca tgcatcagga tgacgacggg
     2221 atagcgcccc ggagtgaccg gatgtgcgag gaaacccggc atgccgccgg ggcattggat
     2281 catttcgccg ctgacgtctt cggacattcg caaactcatg cggtcatcag agcgacagcc
     2341 tgatccgcgt ccatcacgcg ggccatgcgc gggaaaacgc ggtccatgaa aaaatcattg
     2401 ttgttccccc gtgtcgaata gcagcaatcg cgcactacga cgatgccgta atccagatcg
     2461 cgcgccgcga acacggttga ggcgatgccg acatcggtgg aacctccggc gatgacgatc
     2521 gtgtcgatac cgcgggtacg aagctgcaga tcgaaattgg tctggaagaa cgaactccag
     2581 cgctgcttgg gcaccacgac atcgcgtggc cccggcgcca gttcgggcgc gatgtccgca
     2641 tccaccgagc ccttgtggaa atggggccgg atcggggccg cggcgttgcc gccgggcgcc
     2701 agttccatgt cggtgtcggt cagccggtcg acggaatccg cacccgtcgg gtcatgcgcg
     2761 ccggagggat agaacacaat catcccggat ttgcgcgcgc ccgccagcag acgctgcata
     2821 tggccaagaa tgttgcgctc ggcgagaaat cgcagctttg ccggattgtc gcgcggatgc
     2881 agatagccgt tcagcgtgtc gaacagcacc aggccggtgc gtgcggcgct cagttctttt
     2941 tccagccgcg ttattctgcc catggttcct cgcgttcagt gaagcgacag cttgcgcaag
     3001 cgctcgatgg ttttggggga tatacgatag gtgtcggcga tcaattgctg aatctgcgcg
     3061 ccggattttg gcgtgttgac gccgagcgcc atgcgtttgg aatcggcgag gaagcccttg
     3121 tccgcgaagg tgtcggcaag cgccttgcgc atgagggcga cacgcgcagg cgccacgccg
     3181 ggcggcatca gatagggccg ccccaggctc acttcggcaa gtccggcctt cagcaattgc
     3241 cggtcgtccg cattggccgc gaggtcgagc gcggacggca cttccggaat ggcgggttct
     3301 ttttcgagcc catattgcag gatcagcttg atgttctttt cggcgcgcca attgggccgc
     3361 gtggcgttca gcgtgttgta gaaaagactg gggaagccat cgacttcgcc gcgctccatg
     3421 gcgaaataga cttcgttctg gccgggatag ccgaccacga ttttcatctt ggtgccgagc
     3481 gtcgcgttga tcagccgcgc atagaaggaa ggcgtcgaat tggcgcccga cgatcccacc
     3541 gtgatttcgc gtttcttcag atcggcgatg gaattgaccg gcacattgcg ccacaccgcg
     3601 atcaagccta cttcgacgct gggcgagccc agccagttga atttcgtcgc gtcatattgc
     3661 gcttccttgg tgccaagaag cggctcgaag ggtgtgttgt tctgcatgcc ggcgatgaag
     3721 gtgccgtcct tcggcgcatt gcgataaaaa taattcatcg ccacgatgcc gcccgcgccc
     3781 ggcatgttgc tgaccaccac acggggattg cccggaatat gactgcccag atatttggcg
     3841 atggtgcggc ccatgatgtc gtagccgccg cccgcgctcg aactcacgac caaggtgacc
     3901 gttttgccct tgtagaaacc atcgttggat tgagcgcgcg caacgcccgt ccacagcagc
     3961 gacgcggcca gggcaagagc catatggcgt gaaagatttc tcaacctcga actcccgccc
     4021 tgccactcaa cttggccgca ccgtgccttt gacggttgtg cgcagcatca accgcctttc
     4081 ggtcgaagga aaatcctcgc gcgcgtgcga cgagcagcga ttgtcccaca gcagaagatc
     4141 gtccttgcgc cagcgatggg agtagacgaa ttcggtgcgt tccgcctgat cgaacagggc
     4201 accgagaagc cgcatgcttt catccggctc catgtcgagg atcttcacgg tcatcagccg
     4261 gttcacatag acggctttgc ggcccgtgtc ctcatgggta cgaaagatgg gatgcacgca
     4321 ctcgttgaac gcttcggtgc cgcgatcatc gccgcgctgg gtcgagccgt aattatagtg
     4381 atggagcgcc agcctgcctt cgagacgctc gcggatcgcg ggatcgagcg tctcataggc
     4441 ggcatagccg ctggcgaaca gcgtattgcc gccataggac ggaatctcga ccgaatagag
     4501 cagcgtcgcc ttgctcggca cctccttatg gatcatgtcg tgatggaaca tcatctcgcc
     4561 atcgggcagt gcgccaatgg gctcgccgtt ttcccggata ttcgagatca gcatgatgcc
     4621 gggcagcatc cgggaaaaac cgggcgggtg atatttcgcc gggcggtcga gatgtccgtt
     4681 ttcgccaaaa taggtggtga cgcgcaggag gtcctcctgc gagagggtct gtccgggaaa
     4741 caccagcacc agatggtcga gccaagcctg atagatggcg tgaatcgttt cgttgtccgg
     4801 gcgctcacgt aagtccatcc cccggatctc ggcgccgata tgctggctga gcggcacgat
     4861 atcgagccgg gtcttggtca gaacatccat cgccgaactc ccgcggggga ttgctgaatt
     4921 tcgattgtta gagagtaccg aatgggcctg gggccacgca actaggctgc gcgggcgcgg
     4981 atcagatcgg ccgctttttc cgccatcatc agcacggtcg cattgatatg ggccgaaacc
     5041 aggtccggca tggcggaggc atcgaccacg cgcaagcctt ccatcccgcg cactcgcagc
     5101 tcgggatcga gcacgcaatc ctcacgcgta cccatcgggc aagtcgacgc ggggtggtgg
     5161 gcggtgacca ccacgcgctt caaccaggaa tcgatttccg catcggtctg cacctttgga
     5221 cctggcgatt tctcctcgcc acgataggga tcgagcgggg cctgcattgc cacgtcgcgg
     5281 gcgcgcttga aaccttcgcg caaagtcccg aggtcggatg caacggtcag aaggttgagg
     5341 aagatgcgcg ggggcgagcg cggatcggcg ctcgccaatt tgaccgcgcc gcggctttgg
     5401 ggatgcagca gggtggggcg gatgccgaag ccgtccgcgt aggcgggctt caccagcggg
     5461 aaccatagag cagcatttgt cggcgcgccg cggaacatga attcgatatc gggaacggca
     5521 agtccgggac gcgttttcac aaacgcgtgc aggccacccg gaaccactgt tgcgattccg
     5581 gtaccgcgaa aatgcgcctc gaccatgccg cgtgccatgc ggtcgaagcg catggcctcg
     5641 cgaaagggcc ccgggttggg gcggctatag gtgatgagtg ccgccacgtg gtcctgaaga
     5701 ttctttccca ccggcaaatc cacgagcgga gcgatgccga tctcgcgtaa gtgctccgcc
     5761 ggtccgatgc ccgacagcat cagaatttgc ggcgtgttga acgtgcccgc cgaaacgatc
     5821 acttcggcat ccgcgttcgc gcggatggtt tggccaccct gcagatattc cacgccaatc
     5881 gcgcgggttc cttgcgtcaa tatgcgtgtc gtcaacgcgc ccgtgtggac ggtgagattg
     5941 gggcgtttgc gcgcggggcg cagataggcc gacgccgagg acgaacgccg tccgttgcgg
     6001 atggaatatt ggctgcggcc aaagccttca tgcgaaccgg cattgtaatc ctgcacatgc
     6061 gggaagcccg ccgccttggc cgcttcgatc caggcatcga acaatgggtc cgtg
//
LOCUS       DASYEG010000115        17028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1192995, whole genome shotgun sequence.
ACCESSION   DASYEG010000115 DASYEG010000000
VERSION     DASYEG010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 17028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17028
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192995"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VGM72_04585"
     CDS             complement(<1..624)
                     /locus_tag="VGM72_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY4264571.1"
                     /translation="MTISQNRVLIIGAGPVGLCLALKLAKNGIASTIVEQLRDDNFLD
                     QVPRAGTNHPATLEMYDEIGLYEKLEPRGIIAPLFHYWDREKGERIAEFDHAALKDDT
                     RFPYALQCERIKIVEEALKMAKADPLIDVRMGTIFTGFTQNAYGVMAEVENEAGEKES
                     LAARYIVSAEGARSIVRKECGIEFTGFTYPDRTLNIEVAYDFKQHGYT"
     gene            complement(647..1039)
                     /locus_tag="VGM72_04590"
     CDS             complement(647..1039)
                     /locus_tag="VGM72_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4264572.1"
                     /translation="MAKLRHIALIVTDPEASAKFYEQAFDLKRVGQARRGIYLSDGTV
                     NLALLRVEGNEKPGVYHFGAWVEDLAEGEKKATDAGATYLAGRPTSPNSFYECKYRDP
                     DGIVFDITHNGWVGAVKDVVPAKEKADA"
     gene            1181..2488
                     /locus_tag="VGM72_04595"
     CDS             1181..2488
                     /locus_tag="VGM72_04595"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4264573.1"
                     /translation="MRVEISAIEKRTIARVYWRLVPMLFVLMFFNYVDRVNVGFAALR
                     MNRDLGFSASVYGFGATIFFAGYVLLQIPSNLMVHRLGARVWLSLILFVWGAISTATA
                     FVWDAASFYTLRFLLGLAEAGFLPAAALYATYWFPEKYRARAIAGYIIATSCSTIIGG
                     PIAGAIITYMNDVLGLQGWQWMFLLEGAPTIVLAFFVLAWLTDRPSEAGWLDAASREW
                     LVARLAAEKAALAQGTGAGLVTVMKDARVWSLGLLFGSGLVGLYGLILWLPQIIKEMG
                     NLSDLQVGFLSSVPPAIGVVGAIFVSWRSDRVGDRKGHMAGCYIVSGVGLLASALVGD
                     PILAYIFLCIGNGAVLAASPLFWTIAGSFFTGAAAAACIAFVNIVAQVGGIGPWLIGE
                     VRERTGSFTIALITLSGFLFLAALIALLMKAEPKHTAASALAE"
     gene            2508..3044
                     /locus_tag="VGM72_04600"
     CDS             2508..3044
                     /locus_tag="VGM72_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264574.1"
                     /translation="MTDKTRYRAFKRDELTPEQQTVFDAIGGPRSGVVPAPFHVLLES
                     PELASLTQALGAFCRYRTGFPPPLSELLVLITASHWKAEFEFTVHAREAAKAGIAESI
                     IEALRAGRLPDFADAESKLLYDFVTTFFVARDVPDDLFARAVAQFGRRRVVELTGVLG
                     YYSSLAILLRVFRVPADA"
     gene            complement(3211..4248)
                     /locus_tag="VGM72_04605"
     CDS             complement(3211..4248)
                     /locus_tag="VGM72_04605"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4264575.1"
                     /translation="MKDSASDIKIDAYNHFIPPAYLELLKQHSKDIGIVKRMASIPVL
                     CDLDARVELLSRWPEVRQVLTLGLPHPEALGGPEISVPAARQVNDGLAEACKRFPRKF
                     PAFVASLPMNDVPSALEEMDRAIGKLGARGIQLGTNILGRALDDPEFFPVFERMANHH
                     RLPVWMHPLRQQSFADYRSEPKSRYEIWQVLGWPYETSAAMARIVFCGLFDRLPDLRI
                     ITHHCGGMIPYFAGRAETLWAQLGSRTADEDYSQILARMTKPLMEYFRMFYGDTVLGG
                     SSSALRCGLDFFGSEHVVFASDCPFDPEGGAMFIREGIRSVEDLRLTPDVARKIYYAN
                     AAGLMKLTEIK"
     gene            complement(4259..5062)
                     /locus_tag="VGM72_04610"
     CDS             complement(4259..5062)
                     /locus_tag="VGM72_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY4264576.1"
                     /translation="MRTRAIPDQATKTVDSDSRAYVTALARGLSVLKAFDDQNARLSL
                     SDVARIAGLPRASARRALLTLESLGYVENEGRVFSLSPQVLTLARAYLASSPVPRVAQ
                     GFLENVSETLGQSCSLSILNGEEVIYIARSTRKRIGSLHRDVGAHLPAHCTSMGRVLL
                     AATSDAEIGAFLTRVTLRSFTPYTVTDKTELLAILEKVRRNGYSLLDQELEIDLRSIA
                     VPVQNASGRVIAAMNVSAQASRTPKKQLVERFLPALREAAMKMRPLLIG"
     gene            5148..6434
                     /locus_tag="VGM72_04615"
     CDS             5148..6434
                     /locus_tag="VGM72_04615"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY4264577.1"
                     /translation="MLVKEQNDILTLTGAGTAMGQLFRSYWIPALLSSELSEPDCPPV
                     RVQLLSEKLIAFRDSQGRLGLIEEFCAHRRVSLWFGRNEENGLRCPYHGWKFDVTGQC
                     VDIPSELPGSTHCQRIRMVNYPLVEKGGVLWTYMGPPDRTPPLPDWEFTNVAEAHRFV
                     SKRWQECNWLQALEGGIDSSHVSFLHRGDLETDPTFKGAKGNKYNMNDLMPVFDVVES
                     PGGLYIGARRNAENGNYYWRITQWVMPAFTMIPPRGDHPVHGHFFIPMDDENCWTWSF
                     DYRVKSPLEPELVQQMREGAGIHTVIDPRTFRPLANKDNDYLIDRAAQKRGRTYSGVA
                     GFAIQDSSLQESMGPIIDRSRENLVATDKGIVMARRRLLRAAKALADSGTVPPGVDPA
                     HTRVRSASVVLPPDTPFAEAVGDALAARAGAAHISV"
     gene            complement(8015..9079)
                     /locus_tag="VGM72_04620"
     CDS             complement(8015..9079)
                     /locus_tag="VGM72_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY4264578.1"
                     /translation="MRPSLMGMMSNDIAIDLGTANTLIYVRGKGIVLNEPSIVATISK
                     GGRKQVLAVGNEAKAMMGRAPGAIEVIRPMRDGVIADFEIAEEMIKYFIRKIHNRRSF
                     VHPLIIICVPSNSTAVERRAIQEAALSAGARQVFLIDEPIAAALGARLPVSEPTGSMV
                     VDIGGGTTEVAVLSLGGIVYSRSARVGGDSMDEAIVSYVRRRENLLIGETTAEQIKKT
                     IGSASLPLQGDGITMEIKGRDLLKGMPKQMGVTQRQIAEALSESVNAIVTAVRVALEA
                     TPGELSADIAEKGVTLTGGGSLLSNLPRVLSDATGLAVVIAENPLCCVALGIGHVLEH
                     RGNLGHMLAQPAVSLRRSNT"
     gene            complement(9221..9601)
                     /locus_tag="VGM72_04625"
     CDS             complement(9221..9601)
                     /locus_tag="VGM72_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264579.1"
                     /translation="MPDGSADLFKPAQGGELTAPKLHTGPVILAEIRAVLAAHPGGLR
                     RWSVMRAIRKERERTGQDVPQNFEAEVERIFRRFCTDVTAKHSGGAAKDAIFFRPSEK
                     AGEVWAILRDRASSWCNDELTEVI"
     gene            complement(9740..9943)
                     /locus_tag="VGM72_04630"
     CDS             complement(9740..9943)
                     /locus_tag="VGM72_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY4264580.1"
                     /translation="MAIGTVKFFNTSKGFGFISPDGGTKDVFVHATAVEDAGMRSLAE
                     GQRVSFDIQPDAKGSKAVNLKAA"
     gene            10513..10785
                     /locus_tag="VGM72_04635"
     CDS             10513..10785
                     /locus_tag="VGM72_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264581.1"
                     /translation="MGDQDKQSTAQKGAQKYFRKIQDDETLAKHTRKKERAADAVKTA
                     KLRTLRLTKEAADKEAADKQAVENGVSAPSKRRPAMKRAGMVRMTY"
     gene            10834..11307
                     /gene="greA"
                     /locus_tag="VGM72_04640"
     CDS             10834..11307
                     /gene="greA"
                     /locus_tag="VGM72_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY4264582.1"
                     /translation="MERIPMTAAGRKVLENEVAHLKNVERHAIIKQIAEARAHGDLSE
                     NAEYHAAKERQSFIEGRVMDLEDRLSRAEVIDISKLSGKNVTFGATVTLSEEARHAKV
                     KYQIVGDMEADLTKGKVSISSPIARALIGKAAGDTIEVRAPGGARSYEILEVQFV"
     gene            11461..12495
                     /locus_tag="VGM72_04645"
     CDS             11461..12495
                     /locus_tag="VGM72_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4264583.1"
                     /translation="MKMFSDACICSVAAAVVVTAISAYSATAQNARPTPDTVEGHLAA
                     GKNAAGGRDNTPDFYGLVTAICVAPQNGAARPDAPAPRENPDRKATYMEPKKAFDDLY
                     WMGTASTSSWLLTSDDGYILYDTQGVYDAEDVLVGGLRKLGLDPAKVKYVIVSHGHAG
                     ESGGAYLFQSRYGSHVVMNDWDMVAASVNGYPTGKPKRDIVATDGMKITVGNRTVTLY
                     LTPGHTPGTISSVFQVHDNGRPLTVVYSGGTEFNFQNDVTHFDQYLASERRLASLAAA
                     AGATIVLNNQSQFNEAATKLRMLQGRHSGEPNPLDVGTDAVARYFKIEDECAQAVRLK
                     LVAAGITNIR"
     gene            complement(12613..14112)
                     /locus_tag="VGM72_04650"
     CDS             complement(12613..14112)
                     /locus_tag="VGM72_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEY4264584.1"
                     /translation="MPQFENLGDLIRRENDLDKIAIVDLGGEGTPREFTYRMLDDAAR
                     GVARALSRQNIPRGGRVAVLSANRAEYLAAVCGIMRAGLVAVPVNFKFPRETIHFIIK
                     DSGADLVFCDAVRRADCPDGIPTVTFGPEFDAFLDDRPFETLRPVTREPAMFLYTSGS
                     TGIPKGVVLSHQSHLWVVETRLGSADISRHRFLVAAPLYHMNGLAVSQLVSAGHATLV
                     LLPQFTAAAYIDAAERYRCTWLTAVPPMIAMMLRETERMARADLSQVEFVRMGSAPVS
                     EALMTALRHALPEATITNAYGTTEGGPVVFGPHPNGIAQPELSVGYPHPKVQTRLVEG
                     ANRDADHGELEMKSPALMNGYHNRPDLPSPITADGFYRTGDVFRRDENGFHFFVGRTD
                     DMFVSGGENIYPGDVERMLERHPDIAQAAVVPIDDDIKGQKPVAFVVLRDGARLSEDD
                     VKQYALKHAPAFQHPRFVWFIDKLPLSSTNKVDRTVLKQDAAQRLGRAP"
     gene            complement(14124..14552)
                     /locus_tag="VGM72_04655"
     CDS             complement(14124..14552)
                     /locus_tag="VGM72_04655"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY4264585.1"
                     /translation="MSEDIGIEKVQALIARAPFHQWLGIEVVALHDDGIDLSAKWREE
                     WVVNPDRRYTHGGILAALVDLAADWALVKRTGRGVPTIDMRIDYHAAAMPGDLTMKGR
                     VVKAGGQFSTAEAHVYDAGGKLLASGRGTYFTAPPPAPKA"
     gene            complement(14549..15553)
                     /locus_tag="VGM72_04660"
     CDS             complement(14549..15553)
                     /locus_tag="VGM72_04660"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4264586.1"
                     /translation="MKALLLRQHGSLDALELVDNHPVPKAVDGHVVIRVRASSFNYHD
                     VFTVKGMPGIKVPLPVVIGLDMAGEIAELGSGVEGWKTGDRVLVNPLNKKKGLMGEML
                     DGGMAEFCLVAADQLIRMPDGVSFEQAASLPVAYGTAHRMLVTHKTVKAGDKIVILGA
                     SGGVGTGCVELCKLLGAHVIACASSEEKMARLREIGADETVNYLETDFSKWAVEKYGK
                     PQRRTYEGGVDVVINFTGGDTWVPSLRCLKRGGKLLVCGATAGFDPKEDLRYIWSFEL
                     QIIGSNSFYDENLIALMQLIQDGQMKPLIDTVVPLEGAAEGLRLIQDRKVIGKVVVTP
                     "
     gene            15671..>17028
                     /locus_tag="VGM72_04665"
     CDS             15671..>17028
                     /locus_tag="VGM72_04665"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY4264587.1"
                     /translation="MQESFRQFLGRLRDAGELIDLHQPVDIRHIATLVDQSDKALLFH
                     KVIGYDMPVVSGIIRSRERAMLSMSAKTYGEIEVRLKNAIDNPISPIMVNTSPTRDVT
                     MVGEDVDLFKLPIPMSSIYDGGPMITAGVVIAKDPELGWNSGIYRFIVKEKALTGIDI
                     VTPNNMRLFAQRAYEAGRPLPISISIGTHPLEIMASGYRAPLGVDEMHIAGGLRGAPV
                     ELAPCQTVDAPYIADAEIVREGEILPTGWNWPEGRFGEFTRLMGGLHWNPLVRIKAIS
                     HRKDAIYYSLHMPWENTWLAAPTRYAAIRQALRTAGVTVKDINVTLGGCAFWHAVISI
                     KKQPGEAKNAILAALSVMDLKHVVIVDDDIDVFNPMDVEWAIATRVQGDKDILIVTNA
                     RGKPLDPSLPPTPPGVVPTTAKVGIDATIGEGIPKERYERIAYAYADTAKIGDYVKGK
                     SDA"
BASE COUNT         3400 a         5064 c         5122 g         3442 t
ORIGIN      
        1 cgtgtagccg tgctgcttga aatcgtaagc gacctcgata ttcagcgtgc ggtcgggata
       61 ggtgaagccg gtgaattcga tgccgcattc cttgcgcacg atgctgcgcg cgccctcggc
      121 gctgacgatg tagcgcgcgg ccaggctttc tttttcgccc gcttcgtttt ccacctcggc
      181 catcacgcca taggcgttct gggtgaagcc ggtgaaaatg gtccccatgc gcacatcgat
      241 cagcggatcg gccttcgcca ttttgagcgc ttcctcgacg atcttgatgc gctcgcattg
      301 cagcgcgtaa ggaaaacggg tgtcgtcctt cagggccgca tggtcgaatt ccgcgatcct
      361 ctcgcccttt tcgcggtccc aataatggaa cagcggcgcg atgatgccgc gcggttcgag
      421 tttttcgtag agcccgatct cgtcatacat ctccagcgtc gcaggatgat tggtgccggc
      481 ccgggggacc tgatcgagga aattgtcgtc gcgcaactgt tcgacgatag tgcttgcgat
      541 tccattcttg gcgagcttga gcgccagaca gagaccgacc ggccccgcac cgattatcag
      601 cacgcgattt tgcgaaatcg tcatatgtcg aacctatatc ggggattcac gcatccgctt
      661 tttctttggc cggcacgaca tccttcaccg cgccgaccca gccattgtgc gtgatgtcga
      721 acacgatgcc gtcgggatcg cgatatttgc attcatagaa cgaattgggc gaggtgggac
      781 gccccgcgag ataggtggcg cccgcatcgg tggccttttt ctcgccttcc gcgaggtcct
      841 cgacccaggc gccgaaatga tagacgccgg gcttctcatt gccttcgacg cgcagcagcg
      901 caaggttcac ggttccgtcg gagagataaa tcccgcgccg cgcctgcccc acgcgcttca
      961 gatcgaacgc ctgctcgtag aatttggcgg acgcctcagg atcggtgacg atgagggcga
     1021 tgtggcgcag cttggccatg gcggactcct ttaataacga tgccggtctc tccctatagg
     1081 cgagtttggc ggagcgggca aaaggaaggc gagggcagcc tttgcttgta cggcttgtaa
     1141 gcctgcggca cactgcggcc caacgacaaa aagagtgcgg gtgagggtgg agatcagcgc
     1201 gatagaaaag cgaacaatcg ccagagtcta ttggcggctg gtgcccatgc tgttcgtcct
     1261 gatgttcttc aactatgtcg atcgcgtgaa tgtcggcttc gccgcgctgc gcatgaatcg
     1321 ggacctcggc ttttcggcct cggtctacgg cttcggcgcc acgatctttt tcgcgggcta
     1381 cgtgctgttg cagatcccca gcaatctgat ggtgcatcgg ctgggcgcgc gcgtctggct
     1441 gagcttgatc ctgttcgtct ggggcgcgat ctcgacggcg accgcgtttg tgtgggacgc
     1501 ggccagcttc tacaccttgc gcttcctgct cggtctggcg gaagcgggat tcctgccggc
     1561 cgccgcgctc tacgccacct attggtttcc cgaaaaatat cgggcgcgcg cgattgcggg
     1621 ctacatcatc gccacctcgt gctcgaccat catcggcggg ccgattgcag gggccatcat
     1681 cacctatatg aacgatgtcc tcggccttca gggctggcag tggatgttcc tgctggaagg
     1741 cgcgccgacc atcgtcctcg cgtttttcgt gcttgcgtgg ctgaccgacc gcccgagcga
     1801 ggcagggtgg ctggacgcgg cttcgcgcga atggctggtg gcgagactcg cggccgagaa
     1861 ggcggcgctc gcgcaaggca cgggcgccgg gctcgtcacc gtcatgaagg atgcgcgcgt
     1921 ctggagcctc ggcctgttgt tcggttcggg tcttgtcggg ctttacgggc tcatcctgtg
     1981 gctgccgcag atcatcaagg agatggggaa tctcagcgat cttcaggtcg ggttcctgtc
     2041 gtcggtgccc cccgccatcg gcgtggtggg tgcgatcttc gtgagctggc gctcggaccg
     2101 cgtcggcgac cgcaagggcc acatggccgg ctgctatatc gtgtcgggcg tgggccttct
     2161 cgcgagcgcc ttggttggcg atcccattct cgcctacatc tttctctgca tcggcaacgg
     2221 tgccgtgctg gcggcctcgc ctttgttctg gacgattgcc ggatcgtttt tcaccggtgc
     2281 tgcggctgcg gcctgcatcg cctttgtgaa tatcgtggcg caagtgggcg gcatcggccc
     2341 ctggctcatc ggtgaggttc gggagagaac gggaagcttc accattgcgc tcatcacgct
     2401 ttcggggttc ttgttcctgg cagcgctcat cgcgctcctg atgaaggcgg agcccaaaca
     2461 cacggcggca tcggcgctgg cagaataata aggatcgcat cgcaaatatg acggacaaga
     2521 cgcgctatcg cgcattcaaa cgagacgagc tgacgcccga acagcagacc gtattcgatg
     2581 ccatcggcgg ccctcgaagt ggtgtggtgc ctgcgccgtt ccatgttctg ctcgaaagcc
     2641 cggagcttgc gtcgctgaca caggcgctgg gcgcgttctg ccgctatcgc accggctttc
     2701 cgccgccgct ttcggagctt ctggtcctga tcacggcatc ccattggaag gcggagttcg
     2761 aattcacggt gcatgcgcgc gaagcggcca aggcgggtat cgcggagtcc atcatcgagg
     2821 cgctgcgcgc cggacgcctc ccggatttcg cggatgcgga atccaagctg ctctacgatt
     2881 tcgtgaccac gttcttcgtg gcccgcgatg tacccgatga tctgttcgcc cgcgcggtcg
     2941 cgcaattcgg ccgcaggcgg gtggtcgaac tgaccggcgt gctcggctat tattccagcc
     3001 ttgcgatcct gctcagggtg ttccgcgttc cggcggatgc gtaaagcccc tgtcattccc
     3061 ggcgtcgtga tgcgttagaa cagtttgtgt tctgaccgaa tcaaaaaacg attgtcatcc
     3121 cggccgaacg aagtgagagc cgggacccac ggtgcgatgt ctcggtggtc ccggatagtc
     3181 gctgaagcgg cttgcgggat gacatgcagt tcattttatt tcagtgagct tcatcaggcc
     3241 ggcggcattg gcataataga ttttgcgcgc cacatcgggg gtgaggcgca aatcctcgac
     3301 cgaacggatg ccttcacgga tgaacatcgc ccccccttcg ggatcgaacg ggcagtcgct
     3361 cgcgaagacc acatgttcgc tgccaaagaa atccagcccg cagcgcagcg ccgaagacga
     3421 tccccccagc accgtatcgc cgtagaacat gcggaaatac tccatcaagg gcttggtcat
     3481 gcgggcgagg atctggctat agtcctcgtc ggcggtgcgc gaacctaatt gcgcccacag
     3541 cgtttcggcg cgccccgcga agtacgggat cataccgccg caatggtgcg tgatgatgcg
     3601 caaatcgggc agccggtcga acaggccgca gaacacgatg cgcgccatgg ccgcgctcgt
     3661 ctcgtagggc cagccgagca cctgccagat ttcatagcgc gattttggtt cgctgcggta
     3721 atcggcgaaa ctctgctggc gaagcggatg catccagacg ggtagccggt gatggtttgc
     3781 catgcgttcg aacacgggaa agaattccgg atcgtccagc gcgcgtccca gaatattggt
     3841 gccaagctga atgccccgcg cgcccagttt cccaatcgcg cggtccattt cttccagcgc
     3901 ggacgggaca tcgttcatcg gcagcgaggc gacgaaggcc ggaaatttcc gcgggaaacg
     3961 cttgcaggcc tccgccaagc cgtcgttgac ttgcctcgcg gcgggaacag agatttccgg
     4021 cccgccgagc gcttcgggat ggggaaggcc cagggtcagc acctgccgca cctccggcca
     4081 gcgcgagagc aactctacgc gcgcgtccag atcgcacagc acgggaatgc tcgccatgcg
     4141 tttgacgata ccaatatcct tggaatgctg tttgagcagc tcaagatagg ccggcgggat
     4201 gaaatgattg taggcgtcga ttttgatgtc gcttgccgaa tctttcatgg actattcctc
     4261 aaccgatgag cagcgggcgc atcttcatgg cggcttcgcg caaggccggc aggaatcgct
     4321 cgaccaattg ctttttgggt gtgcggctcg cctgcgcact gacattcatc gcggctataa
     4381 cgcgtccgct cgcgttctgc accggaaccg cgatggagcg cagatcgatc tccaattcct
     4441 ggtcgagcag cgagtagcca ttgcgccgca ctttctcgag gatggcgaga agctcggttt
     4501 tgtctgttac cgtgtagggc gtgaagctcc gcaaagtcac gcgcgtcaga aacgcgccga
     4561 tctcggcatc ggaagtcgcg gccaagagca cgcgccccat cgacgtgcaa tgggccggca
     4621 gatgggcgcc gacgtcacgg tgcagcgagc cgatacgctt tcgggttgag cgcgcgatat
     4681 agatgacctc ttcgccgttg aggatcgaga gcgagcagga ttgccccaag gtctcgctga
     4741 cattctccag gaagccctgc gccacgcgcg ggaccggact cgacgccaga taggcacgcg
     4801 cgagcgtcag cacttgcggg ctcaggctga acacgcggcc ctcgttttcg acataaccga
     4861 gcgattccag cgtcagaagc gcacggcgcg cgcttgcccg cggcaggccg gcgatgcgcg
     4921 cgacatccga caggctcagc cgcgcgttct ggtcgtcgaa ggccttcagc acggaaagtc
     4981 cgcgcgccag cgccgtgaca taggcgcggg aatcgctgtc gaccgttttt gttgcctggt
     5041 caggaatggc gcgcgtcctc attcgcgatg cttgactcac ccatgataac ggctacgctg
     5101 tgcgttatcc acatattttg tgcgctatgc gcacattcga ggccgccatg ctcgtcaagg
     5161 aacagaacga catcctcacc ctgacaggtg ccggaaccgc catgggacaa ttgttccgca
     5221 gctattggat tccggcgctc ctatcttccg agctttccga acccgattgt ccgccggtgc
     5281 gggtgcagct cctttccgaa aaattgatcg cgtttcgcga tagccaaggc cgcctcggcc
     5341 tgatcgagga attctgcgcg cacaggcgcg tgtcgctgtg gttcgggcgc aatgaggaga
     5401 acggcctgcg ctgtccgtat catggctgga aattcgacgt caccggacaa tgcgtggaca
     5461 ttccctcgga gcttccggga agcacgcatt gtcagcgcat ccgcatggtg aattatccgc
     5521 tggtggaaaa gggcggcgtg ctgtggacct atatggggcc gccggatcgc acgccgcctc
     5581 ttcccgattg ggaattcacc aatgtcgcgg aggcgcaccg tttcgtctcg aagcgctggc
     5641 aggagtgcaa ttggttacag gcgctcgaag gcggcatcga ttcgagccat gtctcgttcc
     5701 tgcatcgcgg cgacctcgag accgatccca cgttcaaggg cgccaagggc aacaaataca
     5761 acatgaacga cctgatgccg gtgttcgacg tggtcgaaag ccccggcggg ctttacatcg
     5821 gcgcgcgccg caatgccgag aacggcaatt attattggcg catcacccaa tgggtgatgc
     5881 cggccttcac catgattccg ccgcggggcg atcatcccgt gcatggccat ttcttcattc
     5941 cgatggatga cgagaattgc tggacgtgga gcttcgatta ccgcgtgaaa tcgccgctgg
     6001 aacccgaact cgtgcagcag atgcgcgaag gggccggcat ccacacggtg atcgatcccc
     6061 ggacttttcg tccgctggcc aacaaggaca atgattatct gatcgatcgc gcggcccaga
     6121 agcgcggccg cacctatagc ggcgttgccg gtttcgcgat ccaggactcg tccttgcagg
     6181 aaagcatggg gccgatcatc gaccgctcgc gcgaaaatct cgtcgccacc gacaagggca
     6241 tcgtcatggc gcggcggcgt ttgctgcgtg cggccaaggc gctcgccgat tccggcaccg
     6301 tgcctccggg cgtcgatccg gcgcacaccc gtgtgcgttc ggcctcggtc gtgctgccgc
     6361 cggatacgcc tttcgccgaa gcggtgggcg acgcgctcgc ggcgcgcgcc ggtgccgcgc
     6421 acatctcggt gtgaacgtca tccccacccc caatgggagt ggggatactg ctatctccaa
     6481 aacgagccac gctttgggca ccggcaaaag tcgggtgaga tgtgcgaatt cgtttcgagc
     6541 gggatatttt cgaatttcgt tttgaggaaa agcaggcgtt ccggcccatg acctagccga
     6601 gtgttggcgc taccaaccgg gttgtcagac ccacttccct agttgggggt gcgtgccgtt
     6661 acccggcttc gatcgagacc tgcgccgccg cccaccaata aagagtatcc ctcctaggcc
     6721 ggtgaccagg agagtgagtg tactcggctc tgccacgctt gccgggggcg ttacctggcc
     6781 agcgtgccaa gttcctggcg cgccatcgac aaacgccgaa ttgacgagac ccagtccgca
     6841 cagagaattt gaagtgatcg tctgacaacg agccacggta ctaatgtctc tgccggcaaa
     6901 agagaattcg aactgggatc ccatagtccg agcgagatct cctacgttga cggggggcag
     6961 gtaatcttgc tctgcgagca taagccactg ggcaatatcg cccgagctat caacccagag
     7021 gctgagggac gtcaatgtgg agtttgtctg atcaagtctt tgcgctccat cgctgaagct
     7081 aaatatcgac accgcattct gaagaaaata gaattgcgag gtgggagacg gtaaaagcgg
     7141 cgaactcaac tcgaatgaga aagataaacc catattagaa gtataagggg tgaccccagg
     7201 gtccgaaaac ggattgaata tttgatcgta agtattcccc tgataagtgt atacaatcgc
     7261 atttgcggga tgcgtggaaa ccgccaagaa aaatacgaac acaagagcgc cgataccggt
     7321 tcgtaggcct tcaagagcaa taaccccatg ctgccgcccg ttgacgcaca atagcgccac
     7381 cagatttctc gcaaggagga gcattgaggg aggagcggcc cgtcttgtgt ccgttcgcgg
     7441 cattgatcgt tcctctgacc gacaggcacg catttatgtg cgaaaaactt attgttgcat
     7501 aatcaactaa actcagggga cttggacaaa tttgagccag caacgggttg aagctaggaa
     7561 caacaacatt tcgaggaagc tctccgcgag ttttaagtcc gcgtctcatc cgccatagag
     7621 aaaacgcacg ggagcgggcg tggcgaaatg atagaacacc cagcggccca tgtacgccgt
     7681 atcgacaggt cgaacactgg cgagaatgag cctggatatg cggcagccgg agtggccggc
     7741 gcgctccaat ccggccattc cgcctcagct tagaggccaa ccttttctga gatggcgata
     7801 gcgtcatcat cctcgacgcc gaggtaccat acggtgcttt cgagcttggt gccgctgttt
     7861 atgccgcgat tgcgctcttc gtcatggcat gtgttgcggg gatggttcgc agaacatgca
     7921 accgcatcat gcatgcattt gttttcgatc cgcagtcccc aataggatcg gccaagccaa
     7981 tcgcggcggc caacggccac agcgtctacc gatttcaagt attgcttcgc cgcagcgaca
     8041 ctgcgggttg agcgagcata tgaccgagat tcccccgatg ctcaaggacg tgcccaatcc
     8101 caagcgcgac gcagcagagt ggattttcag caatcacaac ggccaatccg gtggcatcgc
     8161 ttaaaacccg aggcagattg gacaacaggg agccaccgcc cgtgagggtt acgcccttct
     8221 cggcaatatc ggctgagagt tccccgggtg ttgcttcgag agcaaccctc acagcggtga
     8281 cgatcgcgtt taccgactcg ctgagtgctt ccgcgatttg gcgctgtgta actcccattt
     8341 gtttaggcat gccctttaaa agatcgcgcc ctttgatttc catggtgatt ccgtcccctt
     8401 gtaatgggag agaagcagag cctatcgtct tttttatttg ttcggcggtt gtttcgccga
     8461 ttaagagatt ttcccgacgc cgcacgtagc tgacaatggc ttcgtccatg ctgtccccgc
     8521 cgacgcgtgc ggaacgtgag taaacgatcc cgccaaggga taaaactgcg acttctgttg
     8581 tgccgccgcc tatatcgacg accatggagc ccgtcggctc actgacaggg agcctcgcac
     8641 cgagcgccgc cgcgatgggt tcgtcaatga gaaatacttg tctcgcgcct gcagaaagag
     8701 ccgcctcttg gatcgcgcgg cgttccacag cggtcgaatt agacggcaca caaatgatga
     8761 tcagtgggtg gacgaatgat cgacgattgt gaatcttacg aataaaatac ttgatcatct
     8821 cttcggcgat ttcgaaatcg gcaataacgc cgtcgcgcat tgggcgaatg acctcaatcg
     8881 ccccaggcgc ccgccccatc atggccttcg cttcgttgcc gaccgcaagc acctgctttc
     8941 tgccgccctt gctgatcgtc gccactattg aaggctcatt gaggacgatc cccttgccgc
     9001 ggacgtaaat cagcgtattg gcggttccaa gatcaatggc aatgtcgttc gacatcattc
     9061 ccatcagact tgggcgcatc ttttactttc cctcttcggc gagacaaaat gttccggcgg
     9121 caagcattga acatggtcag atgccggccg acctcaagga gcggaaagaa ttaattgaga
     9181 tcagaggatc tccccactca acaagggagt gtgcttgatc tcatatcacc tcggtcagct
     9241 cgtcattgca ccacgatgaa gcacggtcgc gaagaatggc ccacacttcg cccgcctttt
     9301 cagagggtcg aaagaagatg gcatctttgg cggcgccacc gctatgtttt gcggtcacat
     9361 ctgtacaaaa ccgccgaaaa atgcgttcga cttcggcctc gaagttctga gggacgtctt
     9421 gccccgtgcg ttcgcgctcc ttacggatag cgcgcatcac cgaccaacgt cgaaggccac
     9481 ccggatgagc ggcaagaacc gcacggattt cggctaaaat gacggggcca gtgtgcaatt
     9541 ttggcgccgt taactcgccg ccttgcgccg gcttgaacag gtccgcgcta ccatcgggca
     9601 ttttgatctc ctcatttggg ataaggactt gtgccagcga ttgggatcca gcgattggaa
     9661 taagatgttg tgccagcgat ccaactgcgg atgacggcag aggattctgt ctctgccgtt
     9721 ccttgatgta aaacggactt tatgcggcct tcaaattaac tgcctttgag cccttagcat
     9781 ccggctggat atcgaacgat acccgttgac cttctgccag gctgcgcatt ccggcatctt
     9841 ccacggcggt tgcatgcacg aacacgtcct tggtgccgcc gtccggtgaa atgaagccaa
     9901 aacctttgct ggtgttgaaa aactttactg ttccgatagc catgtgattg ctcctcacgt
     9961 atcgggttgc gcaccagcga ttgcttgaac gcgcgagaaa cgcaatcatc agttcggaaa
    10021 gttagctgtt tcgtcctggc gcactcaggc gtttggattg tacagtgcgc ttcatctgac
    10081 ggaacgccat cgcgcagcgt gtatacggat ttgtccgcgg acttacaacc cgcgcatggt
    10141 gctatccgcg aagtgttgac gatcgggcgg tctgccgccg catgaacacc gggcaaccgc
    10201 ggcgttttcc agagaaccgc ctagagttgg tactttggcg ctaagcttgt cctcgttcga
    10261 ttcgcaattg tgtttccact atttggaggg agcggcatgg cacgccagac cgtctatgtt
    10321 gtccagacct atatcgccgg caagggcaat cgtacaaacc cacggatttg ccaattctcg
    10381 tgtacagtgg gcccgcggtt actatgtcac gcaagtgctc gctctcgcgc gccatagaca
    10441 atctcgatca tccctgcgga tggtttggcc tgaacggtca ccggactgca tcctctaaca
    10501 aaaggaattc gaatgggcga tcaggacaag cagagcaccg cacaaaaggg tgctcagaaa
    10561 tattttcgta agatccaaga cgacgagacg ctcgcgaaac atacacgcaa aaaagaacgt
    10621 gcagccgacg cggtaaagac cgcaaaattg cgcaccttgc gtttgaccaa ggaggcagca
    10681 gacaaggagg cggcggataa acaggctgtt gagaatggcg tgagtgcccc tagcaaacgc
    10741 cgtcccgcca tgaagcgcgc tggaatggtg agaatgacct actgagcagt cagtatcgat
    10801 tttgccggtc aaaaaagttc gaaggtggag cgaatggaac gcattcctat gacggccgcg
    10861 ggccgtaaag tcctggagaa cgaagttgcg cacctaaaga acgtcgagcg gcacgcgatc
    10921 atcaagcaga tcgcggaagc acgggcgcat ggggatcttt cggagaacgc cgaatatcat
    10981 gccgcgaagg agcgtcagag tttcatcgaa gggcgggtga tggatttgga ggacaggcta
    11041 tcccgcgcgg aagtcatcga catctccaag ctctcgggca aaaacgtgac atttggcgca
    11101 accgttacgc tgtccgaaga ggcgcgtcat gcgaaggtca aatatcagat cgtcggcgat
    11161 atggaggctg acctgacaaa gggtaaagtt tcgatttcct caccaatagc gcgcgcgttg
    11221 attggcaagg ctgccggcga cacaattgag gtcagggcgc caggtggcgc acgctcttat
    11281 gaaatcctag aggtccagtt cgtctgatac caatctgcgc gagcagagcc cagcgccgcg
    11341 aagcttatcc ggatcctcat cggtcatcgc gtgtctcttg gcaacgccgc ttgtcgtcat
    11401 cccagcgaac atcctcgttt gaagtagaca ttggccgcgc ggggtgacat ggggatgcga
    11461 atgaagatgt tcagcgatgc ctgcatatgc tctgtagcgg cggccgtcgt cgtgacggca
    11521 atttcggcat attcagcgac cgcgcagaat gcacggccta cgcccgacac ggtggaaggt
    11581 catcttgcgg ccggcaagaa cgccgccggt ggccgcgaca atacccccga tttttacggt
    11641 ctggtcactg cgatctgcgt cgctccgcag aatggagcag cgaggccgga tgctcccgcg
    11701 ccgagggaga accccgatcg caaagccaca tacatggagc cgaaaaaggc cttcgacgat
    11761 ttgtattgga tgggaaccgc gagcacttcg agctggcttc tgacctccga tgacggttac
    11821 atcctgtacg acacccaggg cgtctacgac gccgaggacg tgctcgtcgg cgggctgaga
    11881 aaactcggcc tcgatcccgc caaggtgaaa tacgttatcg tttcccacgg tcatgccggg
    11941 gagtccggcg gcgcctatct gttccagtcg cgctatggct ctcatgtcgt gatgaacgat
    12001 tgggatatgg tcgcggcatc cgtcaatggc tatccgacgg gcaagccgaa acgcgacatt
    12061 gtcgcgacag acggcatgaa aatcacggtc ggcaaccgaa cggtcacgct ctatctgacc
    12121 ccgggccaca cacccgggac gatctccagc gttttccaag tgcacgacaa tgggaggccg
    12181 ttgacggtcg tctattcggg cggcaccgaa ttcaatttcc agaacgacgt gacgcatttt
    12241 gaccaatacc tcgcctctga gcggaggctt gcctcactcg cggccgccgc cggcgccacc
    12301 atcgttttga acaatcagtc tcagtttaat gaagcggcga ccaagctgag gatgttgcag
    12361 ggtcgtcact ctggggagcc caaccccttg gacgttggaa cggacgcagt ggcgcggtat
    12421 ttcaaaatcg aagacgaatg tgcacaggcg gtgcgcctga agctggtcgc ggctggaata
    12481 acgaatattc gataaccgcc gcgggctgtc actattgcgt tgcagacttc atgagcggag
    12541 cggcagcgat ggaccgcacg gcttttccga aaaatctgat cggcgaatcg gttgtgctat
    12601 ggcaaggatg cgctaagggg cacgccccag ccgctgtgcc gcgtcctgct tcaagactgt
    12661 tcggtccacc ttgttggtgg aggagagcgg caatttgtcg atgaaccaga cgaaacgagg
    12721 atgctgaaag gcgggcgcat gtttcaacgc gtattgcttc acatcgtctt cgctcagtcg
    12781 cgcgccatcg cgcaacacca cgaacgccac cggcttctga cccttgatgt cgtcgtcgat
    12841 gggcaccaca gcggcttgcg cgatatcggg atgccgctcc agcatgcgct cgacatcgcc
    12901 gggatagatg ttctcgccgc ctgacacgaa catgtcatcg gtgcgcccga cgaagaaatg
    12961 gaaaccgttt tcgtcacgcc ggaacacatc gccggtgcga tagaatccat ccgcggtgat
    13021 gggcgaggga agatcgggcc ggttgtgata gccgttcatc agcgccgggc ttttcatttc
    13081 cagctcgcca tgatccgcat cgcggttggc accctccacc agccgcgtct gcactttggg
    13141 atgcggatag ccgacggata attccggctg ggcgatcccg ttggggtgtg ggccgaacac
    13201 caccggcccg ccttcggtgg tgccataggc attggtgatg gtggcctcgg gcagcgcgtg
    13261 gcggagcgcc gtcatcaggg cttcactcac cggggcggaa cccatgcgca cgaattcgac
    13321 ctgggataga tcggcgcgcg ccatgcgctc cgtctcgcgc agcatcatgg cgatcatcgg
    13381 cggcacggcg gtcagccagg tgcagcggta gcgctccgcc gcgtcgatat aggcggcggc
    13441 ggtgaattgc ggcagcagca ccaaagtggc atggccggca gagacaagct gcgacacggc
    13501 aagcccgttc atatgataga gcggagcggc gacaaggaag cgatggcgcg agatatccgc
    13561 cgagccaagc cgcgtctcga ccacccacaa atggctttga tgcgacagca ccacgccttt
    13621 agggatgcct gtcgagcccg aggtgtaaag gaacatggcc ggttcgcgcg tcacggggcg
    13681 caatgtttcg aatggtcggt catccaggaa cgcatcgaac tccgggccga atgtgaccgt
    13741 cggaatcccg tccgggcaat cggctcgccg tacggcatcg cagaacacca ggtcggcgcc
    13801 cgaatccttg atgatgaaat ggatggtctc gcgcgggaat ttgaaattca ccggcaccgc
    13861 cacaaggccc gcgcgcatga tgccgcaaac cgccgcgaga tattccgcgc gattggccga
    13921 aaggaccgcg acccgtccgc cgcgcggaat gttctggcgc gataacgcac gcgcgacgcc
    13981 gcgcgcggca tcgtccagca tgcgataggt gaattcgcgt ggggtcccct cgcctccgag
    14041 atcgacaatc gcgattttgt cgagatcgtt ctcgcgccgg atcagatcgc cgagattttc
    14101 aaattgcggc atcgacgaag gggtcacgct ttcggcgcag gcggcggcgc ggtgaaatag
    14161 gtcccgcgcc cgctcgcaag cagcttgccg cccgcatcat acacatgggc ttcggcggtg
    14221 gagaactgac cgcccgcttt caccacgcgc cccttcatcg tcagatcgcc cggcatggcg
    14281 gcggcatgat aatcgatccg catgtcgatg gtcggcacgc cgcgccccgt gcgtttcacc
    14341 aaggcccaat cggccgcgag atcgaccaat gcggccagaa taccgccatg ggtgtagcgc
    14401 cggtcgggat tgacgaccca ttcctcgcgc catttcgcgc taaggtcgat gccgtcgtcg
    14461 tgcagcgcca cgacctcgat ccccagccat tggtgaaagg gcgcgcgcgc gatcagcgcc
    14521 tgcacctttt cgatgccaat gtcctcgctc atggcgtcac caccaccttg ccgatcactt
    14581 tgcggtcctg gatgaggcgc aagccctcgg ctgctccttc gaggggcacc accgtgtcga
    14641 tcagcggctt catttggccg tcctggatca attgcatgag ggcgatcagg ttctcgtcat
    14701 agaaactgtt cgagccgatg atctgaagtt cgaagctcca gatgtagcgc aggtcctctt
    14761 tcggatcgaa gcccgcggtc gcgccgcaga cgagcagttt gccgccgcgc ttcaaacatc
    14821 gaagcgacgg cacccaggtg tcgccgccgg tgaaattgat caccacatcg acgccgccct
    14881 cataggtgcg ccgctgcggc ttgccatatt tctcgacggc ccatttggag aaatcggtct
    14941 ccagataatt caccgtctcg tcggcgccga tctcgcgcag cctggccatt ttctcttcgc
    15001 tgctggcgca ggcgatgaca tgggcgccga gcaatttgca cagttcgacg cagccggtgc
    15061 ccacgccccc cgacgcgccc aggatcacaa ttttgtcgcc cgccttcacg gttttatgcg
    15121 tcaccagcat gcgatgcgcc gtgccatagg ccacggggag cgaggccgcc tgctcgaagc
    15181 tcacgccatc gggcatgcgg atcagctgat cggccgcgac caggcagaac tccgccatgc
    15241 cgccatcgag catctcgccc atcaggccct ttttcttgtt cagcgggttc accagcacgc
    15301 ggtcgccggt tttccagccc tcgacaccgg acccaagttc cgcgatttcg cccgccatat
    15361 cgagcccgat caccacgggc agcggcactt tgatgccggg catccctttg acggtgaaaa
    15421 catcgtggta gttgaaggac gatgcccgta cgcgaatgac gacgtgaccg tccaccgcct
    15481 tgggcacggg atgattgtcg accagttcga gggcatcgag actgccatgc tggcggagga
    15541 ggagggcttt cattctttct tggcctgcgg ggtcgatggg ttgaaactct atctagcccg
    15601 ccagcccccg attggctagg aacaggcgta aatttccgcg cgaaatcggc agcggatcag
    15661 ggagatcgcg atgcaggaaa gtttcaggca attcctcggc cgcctcaggg atgcgggcga
    15721 gttgatcgat cttcatcagc cggtggacat ccgccatatc gcgacgctgg tggaccaatc
    15781 ggacaaggcg ctgctgttcc acaaggtcat cggctacgac atgccggtgg tctccgggat
    15841 catccgctcg cgcgagcgcg cgatgctgtc catgagcgcc aagacctatg gcgagatcga
    15901 ggtgcggctg aaaaatgcca tcgacaatcc gatttccccg atcatggtga acacctcgcc
    15961 cacacgggac gtcacaatgg tgggcgagga cgtggatctg ttcaagctcc cgattcccat
    16021 gtcgtcgatc tatgacggcg ggccgatgat caccgcgggc gttgtcatcg ccaaggaccc
    16081 cgagcttggc tggaacagcg gcatctaccg cttcatcgtc aaggaaaagg cgctgaccgg
    16141 catcgacatc gtcacgccca acaatatgcg cctgttcgcg caacgcgcct atgaggcggg
    16201 ccggccgctt cccatctcca tcagcatcgg tacgcatccc ctggaaatca tggcctcggg
    16261 ctaccgtgcg cccctgggcg tggatgaaat gcatatcgcg ggcgggcttc ggggcgcgcc
    16321 cgtcgaactc gcgccctgcc aaacggtgga cgcgccctat atcgccgatg ccgaaatcgt
    16381 ccgggaaggc gaaatcctac ccacgggctg gaactggccg gaaggccgct tcggcgaatt
    16441 cacccggctg atgggcgggt tgcactggaa tccgctggtg agaatcaagg cgatctccca
    16501 tcgcaaggac gcgatctatt attcgctgca tatgccgtgg gaaaacacct ggctggccgc
    16561 gccgacgcgt tatgccgcca tccggcaggc gctgcgcacg gccggcgtga ccgtgaagga
    16621 catcaacgtc acgctcggcg gttgcgcctt ctggcacgct gtcatctcga tcaagaagca
    16681 gcccggtgag gcgaaaaacg cgatcctcgc ggcgctctcg gtgatggacc tgaagcatgt
    16741 ggtgatcgtc gatgacgata tcgacgtgtt caatccgatg gatgtggaat gggcgatcgc
    16801 cacccgcgtc cagggcgaca aagatatcct catcgtcacc aatgcgcgcg gcaagccgct
    16861 cgatccctcg cttccgccga cgccacccgg cgtggtgccg acgacggcca aggtcggcat
    16921 cgatgccacc atcggcgaag gcatcccgaa ggaacgctat gagcggatcg cctatgccta
    16981 tgccgacacc gcgaagattg gcgattatgt gaagggcaag agcgatgc
//
LOCUS       DASYEG010000116        65113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2352921, whole genome shotgun sequence.
ACCESSION   DASYEG010000116 DASYEG010000000
VERSION     DASYEG010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 65113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65113
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2352921"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VGM72_04670"
     CDS             complement(<1..338)
                     /locus_tag="VGM72_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264588.1"
                     /translation="MKRKSPKECGLPATIARFQRAASLAPASEAARYALGCAWLDAGE
                     AEHAIEILSSLSDIQSPYAERAAAKIAEGRAMLQAKRSPAPYVRHLFDQFAERYDRQM
                     LDDLSYCAHR"
     gene            complement(684..2546)
                     /locus_tag="VGM72_04675"
     CDS             complement(684..2546)
                     /locus_tag="VGM72_04675"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY4264589.1"
                     /translation="MKFDISRALDMTLFSSFRSYGDPVFPDSLPQRLRSFSILADVGD
                     HALRRLLAEADWFGLPGGTQLPRDGENDRAVFLVVTGSLGVFVDEDNAPKRLVATIPA
                     GETVGEMSLLTGESHSATLIALRDTELLRLGPKAFEMLLTRYPRVMLNLLKVVVRRLR
                     ETTRGGHQRTRPKTFAIVPLQHGLADDPIARSIAAILEEMGAKAAVLDKSMSEQPTDW
                     FNRFEAEHDVVFYQGDQPESTWSQFCLRQADRVLLIARADRNLPLHPFERRYFKRETS
                     NPPELLLVHASDASRRGVPEHIELRSDLFGTHHHLRAGNMSDMKRLARFIAGSAVSVV
                     LAGGGARGFAHIGVLKALKEAGVPFDYVGGTSMGGIVAAGIAMEWDIDELSERVRDAF
                     VVNKPLSDFTVPLIALFRGAKVSALLQKHFGDIRIEDLAKPYFCVSSDLTSGRDYVHR
                     SGLLWRALRASVALPGILPPVTEGGHLLVDGGVMNNLPVDVMAAEARGPIIAVDVAGE
                     IDLHAEDARYGERSIFSLIGQRMRGSPSIISILMRAGTVGSDLQRRSVRAQADFLYEP
                     PLEGVGMRDWKFFEQAIAQGYAHAMLEIEKHGVPLSDSWTAGPALAAGHILAPG"
     gene            complement(2548..3339)
                     /locus_tag="VGM72_04680"
     CDS             complement(2548..3339)
                     /locus_tag="VGM72_04680"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4264590.1"
                     /translation="MNPQALFRLDGRVAFLSGAAGHLGRAMALALGQAGAKLIINGRN
                     AERLAAFACELENDGIATECAAFDMMDFETVRSFFAAQRCLDILINNAVTMSPKALAQ
                     VTPEDFDTAYRSAVTAAFEASRAACPALTRAAAERGDASIVNIATMYASVSPDPKLYS
                     APGQMSPPHYGAAKAGLVQLTRHLAAELGPSGIRVNAVAPGPFPREAVLEADPAFAAR
                     LAARTMLNRIGKPSEIAGPVLFLASPASSFVTGALLAADGGWTAW"
     gene            complement(3336..4268)
                     /locus_tag="VGM72_04685"
     CDS             complement(3336..4268)
                     /locus_tag="VGM72_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY4264591.1"
                     /translation="MGIARRAELVMGSAQIGLDYGAANRTGKPAREAALRLVRRAADR
                     GVVTFDTARAYGDAEERLGEALDGRRSVRTITKLSALGDLAADASREAVMAAVDASIG
                     QSLAALRRTSLDCLLLHRARHITAYRGVVWARVKDHLASGVIAALGVSVQSPAEAHEA
                     LREASVTHIQLPFNLLDWRWRDAGVIAALRSRPEVTVHARSIFLQGVLIAGDPRLWQR
                     VPGVDGPRLIAWLEETARAFGREGVADLALAYARGQDWIDGVVIGQETEAQLDGNLRL
                     CDRPVLAPEDCARIAARAPRVPESLLDPAQWTPQ"
     gene            4380..4913
                     /locus_tag="VGM72_04690"
     CDS             4380..4913
                     /locus_tag="VGM72_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY4264592.1"
                     /translation="MKRLIILILALAIGACATRPPGPELPSSEAQMSALKERLFVLVE
                     AERHRLNSEARPLILDPELMRAAQTHSDDMASKHSFDAGNPNGNLAIATLLADPKFRG
                     FVGENSAAQYFTPGARIDPDAYARGFLDIWLKSTDHKMNLIYPAFDKTGIGIAVSGDM
                     IYAAELFATDLGLPEPQ"
     gene            4934..5281
                     /locus_tag="VGM72_04695"
     CDS             4934..5281
                     /locus_tag="VGM72_04695"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HEY4264593.1"
                     /translation="MTNTARIKTYGEFWPFYLREHAKPETRLWHIVGTGTASVLVAAA
                     VFSVSYELLLAAMVTGYGPAWFAHFLIEKNRPATLRYPIWSLVSDFRMAGAWLTGDLA
                     RELRDAGVEGPVD"
     gene            5393..7561
                     /locus_tag="VGM72_04700"
     CDS             5393..7561
                     /locus_tag="VGM72_04700"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264594.1"
                     /translation="MKHTLPWNVTGIPPEVREIARAAALREGISVGEWLTRRITTDAG
                     KLTGGEPQAPMASPVRSPREDLHPDTSDPVFRRIDEAFRNLAHRLENTERAQTDAHRA
                     MRAAAHEINAATRDQAEAFQSITTRIDRVERQTDTGALRDAVRGLHQGLSRLAEQIAK
                     TTNESSNGITALAGNVEMLAGRMANAGEESQQNARALEERFAVLDERIRDSEGRLKQT
                     EERLAAALRSDGTVAMFQSRLASTEQRMRESLDNSLSGFERSLKQMGERLQKTEMHDG
                     SDNRIDEALQTLAVRVGAMESRQSFPVLESAAGDTLSLIQAAVDGALPSGAPDLAEFR
                     LTPDASLAPASTPAAGMPPLAHEIDAPDCVGTIGDAKFHRADEVQPARASAENFLAQA
                     RRAAMRATERSASHGTRHRIGVPAGSGPGLRQRISQHASMTFLFLVLMGAGFLLTRAV
                     QNRAEVLAFDPSHLIALAAPASAGPMTAPEKTSAPAIPAAVPRQAVESVAPALAAPAD
                     SVASASVLPFGAAGETPLAQLLAKANSGDPKAAMSLGLEYADGDGVAANDTEAFYWLQ
                     KAAEAGEAVAQYRLGTLYERGRGVPADTRQALHWYGEAAKRGNRRAMHNLAIVYADGA
                     AADRNFPEALRWFKDAAELGLTDSQFNLAVLYERGLGVKPSLSEAYKWYEIAAASGDN
                     QSKARVAALMNQITPEDRDTADKAARAYRPRPIDLAANDG"
     gene            7658..8578
                     /locus_tag="VGM72_04705"
     CDS             7658..8578
                     /locus_tag="VGM72_04705"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY4264595.1"
                     /translation="MEVYLPVAELSVNWAMILGMGAAVGFLSGMFGVGGGFLMTPLLV
                     FYGIPPGIAVATQASHITASSLSGALAQWRRQGVDAKMGLMLLAGGLLGSAGGVYVFS
                     ILQKLGQIELVVTGSYVILLGSIGGLMLNESIRTIRAARSGKAQPFAKPSQHSWIHGL
                     PFKMRFRRSKLYISVIPPLVLGVLIGVLTAIMGVGGGFIMVPAMIYLLRMPTNVVIGT
                     SQFQILFVTAATTILHATSDQTVDIVLAFLLVVGGVVGVQLGIRVGSQLRGEQLRALL
                     AILVVAVALRLLVGLITRPGDIYSIIAGTP"
     gene            8638..9381
                     /locus_tag="VGM72_04710"
     CDS             8638..9381
                     /locus_tag="VGM72_04710"
                     /inference="COORDINATES: protein motif:HMM:NF021148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02186 family protein"
                     /protein_id="HEY4264596.1"
                     /translation="MVFLAAFFPGVGARADDLVSGLSTDLIQITSNFTGTDIVLFGAI
                     EPTEEAGPAKDQDLVVVIRGPQVDMTVRRKARVFGIWVNREQTVFTGLPGYYFLASTR
                     PLDDIASLATQQRFRLGTANLDSPVLGGMKPDEAAAFRAAAIRDRKRERLYWESPTGI
                     EFLSRTLFRARIAVPASVPPGEYRAEVYLFQNGTVVSAQSSPLYIDKSGFERRVYNYA
                     YQASFAYGFAAVLMAFAFGWAGFLVFRER"
     gene            complement(9456..10628)
                     /locus_tag="VGM72_04715"
     CDS             complement(9456..10628)
                     /locus_tag="VGM72_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005170900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY4264597.1"
                     /translation="MADFDWADPLLIEQELSGEERMVRDSARRFCEGELAPRVREDFR
                     HERFDRKLMEAMGAMGFLGATLPEEEGGAGLNNVAYGLIAREVERVDSGYRSLISVQS
                     ALVMHPIHAYGTPAQKKKYLPKLAKGELLGCFGLTEPDHGSDPGSMSTRAVKAPGGFR
                     LNGAKTWITNSPYADIAVVWAKSEDDRIRGFIVERGTKGFSTPKIEGKFSLRCSPTGE
                     IVLEDCFVPEENMFPEVKGLAGPFGCLNKARFGIVWGAMGAAESCWHAARQYTLDRKQ
                     FGRPLAANQLVQKKLADMQTEIALGLGGALRLARLMDQDKATPEMISLLKRNNCGKAL
                     DIARIARDMHGGNGVADEYNVIRHVLNLEAVNTYEGTHDIHALVLGRAQTGIQAFN"
     gene            complement(10650..11405)
                     /locus_tag="VGM72_04720"
     CDS             complement(10650..11405)
                     /locus_tag="VGM72_04720"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY4264598.1"
                     /translation="MTYYVTVSDGTDIAYDCMGDGLPLVLIHGFGANRNITWSNTNWY
                     QTVQRARHKLIAIDCRGHGESEKPHEPADYDEERMAMDVIAVLAALEIPEVDVMGYSM
                     GAQIAIRLMHDAPGRVRRTILAGIGENYFRPSKDAVESIAAGLLAKDATEVTDPVARE
                     YRSFCEKAGDDLVAMAACIRRPRRIFPPEELRALAQKVLVVCGEQDDTAGSPGPLSDA
                     FRDAATLIVPKRNHHSTVGDRSFKDAVVAFLAN"
     gene            11483..11848
                     /locus_tag="VGM72_04725"
     CDS             11483..11848
                     /locus_tag="VGM72_04725"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEY4264599.1"
                     /translation="MNGWLLVGAVNGFLAVAAGAFAAHGLEGRVSLRALEIFQTGAGY
                     HLTHALAIVATSFVGGARGARAGVAAWLFTLGIVLFSGSLYFMVLADSTALVLATPLG
                     GVCLLAGWAALGFAALKHD"
     gene            11890..12774
                     /locus_tag="VGM72_04730"
     CDS             11890..12774
                     /locus_tag="VGM72_04730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039989.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY4264600.1"
                     /translation="MPPSNPTDSFSYATEDDPRLKRLLIRAIERMTGQPHLKRLYDEH
                     RTNPVPGESFWAAAIRNLELNVVCNDDALAQLPATGPLVIVANHPFGVLDGLIISYLT
                     SRVRNDFLVLTNSVLYQADEIREHLLPIDFAETKEALGTNLKSRAAAKAHVVKGGCLV
                     IFPAGGASTVPKPWSKRAVDPEWKNFSARLIVSAKAPVIPVYFAGQNSRLFQVASHIS
                     MTLRLSLFFKEVYDKIGSEIHVRIGGLIPYKELEHLDRKDLMEHLRKATYALGADLPD
                     AEIRGKRPRPFPPPGKRS"
     gene            12771..13547
                     /locus_tag="VGM72_04735"
     CDS             12771..13547
                     /locus_tag="VGM72_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY4264601.1"
                     /translation="MNAPNNKGANPHVGLLVTCLADMFRPEVGFAAVKLLEQAGCDVE
                     VPAQSCCGQPAYNAGDAKDAAALAKQVIANFEAFDFVVVPSGSCGGMAKVHYPHLLKD
                     DPAWALRANALAAKTHELFSFLVNVRGMKNVAAACNGRAAYHDSCSSLREMNVSGEPR
                     LLLQSVDGLSVRDLAEKETCCGFGGFFSVKYPEVSARMADDKIADAKATGAQMILAGD
                     LGCLLHLAGRLSRKGEALHVRHAAEVLAGMTDGPAIGEAE"
     gene            13544..14977
                     /locus_tag="VGM72_04740"
     CDS             13544..14977
                     /locus_tag="VGM72_04740"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HEY4264602.1"
                     /translation="MSTDASQFPREARAALHDVRLQGALAHLKEGFQHKRAAAFAGFP
                     IPFEALREQGRAIRDSALSQLDGLLLQFETQVEAAGGHVHWARDAAEARDVVTGILKQ
                     AGAKTVTKGKSMVTEEIELNAHLVAQGITPVETDLGEYIIQLRDEKPSHIVAPAFHLQ
                     RSDVEESFRTAHQSLPEARSLDDRPALVGEARAVLRRHFEAADAGITGANFLIAETGT
                     AIIVTNEGNGDLTRLLPRTHIVVTGIEKIVPRLADAAILLRLLTRSATGQEITSYVSF
                     MSGPKRKEDADGPENFHVVLLDNGRSALLSSPAREVLQCIRCGACMNHCPVYGAVGGH
                     AYGATYPGPLGAALDPGLDGLTKTYHLPNASSFCGRCETVCPVKIPLTRIMRHWRNEA
                     FARGIPSWSFDAGLKMWSWIARHPPLYAMGTAIAARIMRAIAGKHPRLRSLPILSGWF
                     AARDLAKPAAVSFQARWHSRRPGKRHP"
     gene            14974..15582
                     /locus_tag="VGM72_04745"
     CDS             14974..15582
                     /locus_tag="VGM72_04745"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEY4264603.1"
                     /translation="MSAARETILQAIAKGRGVAKPVPDYILPSWPGETGAHFITKAKA
                     SIAAVHEIVSPEDAPETILSILIADGLTSRLHLPAASPLNALPWHRAPGLTLLPEPPD
                     GNDSAFSMADYGIAETGTLVFFSGPRSASSWHFRPGCEFVLLSRNAILPRVEDIIALI
                     APWKSMPATLNLVTGPSRTADIEQTIELGAHGPRALHILIAG"
     gene            15717..16850
                     /locus_tag="VGM72_04750"
     CDS             15717..16850
                     /locus_tag="VGM72_04750"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEY4264604.1"
                     /translation="MRAQQSLWTAHQGWTGHSGGPADLLLAFGGTRAVTDRDLWNELA
                     SRHPGAILLGCSTGGEIHGSDVLDESLSVTSLSFARTPLRSAEAIVGSAADSFKAGQE
                     IGKSLYALDLKTIFVLSDGTRVNGSELVRGIRTCTGNKVIITGGLAGDGPRFGTTYVG
                     LNGTPIPGRVAAVGFYGEAIAVGHGSAGGWDVFGPERNITRSKGNVLYELDGKPALDL
                     YKHYLGEDAERLPGSALLFPLKVYPRDCAGEALTRTIVAVSETDRTMTFAGDMPEGFS
                     AQLMRGNFDRLVEGAADAAQQAALSQLGDQVAILVSCIGRKLLLGQRICEEVEAVQDV
                     FGQKTALTGFYSYGEVSPHATTHCAELHNQTMTITVFGESTGA"
     gene            16843..18876
                     /locus_tag="VGM72_04755"
     CDS             16843..18876
                     /locus_tag="VGM72_04755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4264605.1"
                     /translation="MHKLLARQLRAATKEGAGIDLDALIAIVDQTYAEFDRERRLNDR
                     AATLMEDELKAANAEAKREHDIVLAAILDNASDGMLVLRGNGEIVIANAAAEKQFAAS
                     VGALTGMRIAMLLGEDAHAIARGEYGQGAMPELSGMTLDGRAFPIEYSCADLDVSGDR
                     RQLWMIRDISERVRAQREIMESRMRFQDFAEASSDCFWEMDANLKQVEVSSALQSEPV
                     ANLSSLLVPGPDGLTPKGVVEEGWRAFRQNLSARQRFRLRLDITGSDGEILYVAVGGK
                     PVFDLDGGFRGYRGTARDVTREVAAREAARRAERRLIEAMDAAPCAVALLDSRLRLVS
                     GNSALRTMASAKGGRLPFGGSFQDFLGGLKADDIAPQSLRGLVQTGEMREMAIGGSWY
                     LAAAGSLSDGGMVLTFSDVTALKERERELAESKLSAESASRLKSQFLATMSHELRTPL
                     NAILGFSEVIRDGVFGRSQNAWGKYAEYASSIHASGRHLLSLISEILDLSKIEAGSYV
                     LDLRTIDLHEVVEDALTIVSPAAMKAGVQLCPILPAGEIWLTADERAVRQIALNLLSN
                     AVKFTPGGGSVTIEVSTAGEEAKFTVTDTGIGIDKAHVSTVFELFRQVDSSLQRRHEG
                     TGLGLAITKRLIELHGGTISLESELAVGTKVSVTFPDALARTSSAPILKGAAA"
     gene            18966..20240
                     /locus_tag="VGM72_04760"
     CDS             18966..20240
                     /locus_tag="VGM72_04760"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4264606.1"
                     /translation="MSESPPDVPSLPPWLMRLPRVFRHRNYRLFYAGQGVALMGAWVQ
                     TVALSWLVYRLSHSVFLLGLVSFVAQAPIFFITPFAGMIADRHDRRFVFMITRALCMI
                     QAAILTVLTLTDNAGIGTIIALALVLGIITAVETPVRQSFTVEMVGREDLRQAIAFNA
                     MMFNLARTLGPAIGGVIVAVLGEGLCFLLNTLSYGAVLTSLFLMRTKSRTGRPTTHPW
                     DDLKQGFQYVTRHPHLRMVLFLSATCSCFGTSYLALMPAFTQDILHQGAEALGYLISA
                     FGVGAISGAILVSRLHERHLALTPTIMAVLLGASLIIFANTQSMLLALLFVVPTGLGY
                     LGIAVSSNTQVQTLSDDGMLGRMMAFYAMGALGTPPFGALLLGYISDLVGVRSAFMLS
                     GAICLIAAGLSLMSLRRRGLVDFAVLREAQEP"
     gene            20237..22552
                     /locus_tag="VGM72_04765"
     CDS             20237..22552
                     /locus_tag="VGM72_04765"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4264607.1"
                     /translation="MSVNADFDGLKRIREAIALHQQGRLAEAERLYRDILAHTPDQPD
                     ALHFLGILEGQRGRHQPALQLLDRALAVNPRHPAIAYNRANLLRDMGRLEDALTGYDT
                     ALAIKPDNVLALGNRGAVLHGLGRYDDAMASYERALALKPGDCDTHSNRANALAALGR
                     HEDALAGYDKALAGKPGHVAALYGRGNALAKLGRPEEALASYDRAVQCDPGNAELLAN
                     RAIVLSDLNRYEEALAEFASSIARAPENAESFNSRGLVFMQLRRHRDALADFTRACEL
                     NPAFANALYGRASALVELNRHDEAIPAFEQLLRQAPDYPYGLGMLVYAQRTACDWRDL
                     SASAALADAIAAGKRVATPLVLLAISDSPSLKLRCAEVLIADKFRPVEEPLWRGDRSR
                     HERIRVAYLSADFRPHPVAILMAGVFEHHDRERFETIAISYGTDDKSMMRARLMQSFE
                     RFIDVRGKSDRQVAALIHDMEADILVDLTGLTASARPGILAFRPAPVQVNYLGFAGSL
                     GAPYTDYILADTIVIPEDQQVFYAEKMAYLPASYLPHDSKRRIAERAPARGELGLPDS
                     GFVFASFNNSYKFTPEIFDVWMRLLRKIEGSVLWLSASNAAAQRNLKREAEARGVAGH
                     RIVFAGFVPQGEDHLARLAAADLFLDTFPYNAHTTASDALWAGLPLLTCKGQSFASAV
                     AASLLQACGLPELITDSLAAYENRALDLARNAAALADIRAKLSSNRDVCALFDTARFT
                     RNLERAYTTMWKRSQSGTEPTSFHVEPVADP"
     gene            22549..24744
                     /locus_tag="VGM72_04770"
     CDS             22549..24744
                     /locus_tag="VGM72_04770"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4264608.1"
                     /translation="MSEAALKQAWSLQQAGQHGEAARLYADVLRTNPRNFDALFQLGN
                     IYLVTGHPADSERLYARAAQINSQSPELFYNRGCAAMSLSRHEDALAAFAHALALRPD
                     FTEARNNRGVTLLSLKRHKEALACFDRVLADRPGLATVQNNRATALLGLLRQEEALAA
                     AELALCENPQDAHALYNRGAALMMIGRLPESLSQFDKALSVNRDYADALIYRGIVLAV
                     LNRHEEALASYNMALVIRPGDIEILYNRATSLWALKRFEEAIPDCEEVLKRNPHFKYA
                     RGNLVQSRLQCCDWQNLTEDKVKIVTDLHNGVLTLNPLQSIVLFDSPDMLLQAARLWI
                     ANECAPAMTPLWQGEIHKHDRIRIAYMSADFRMHAVALLVAGVFEHHDRTRFETTAIS
                     IGPNDSSPMRARLETAFDRFIDVQDRTDAEVAELIREMEIDILIDLTGFTQHGRTGIL
                     ARRPAGIQVNFLGFPGTMGAPYIDYILVDETVIVEADQRYFEEKVAYLPETYLPNDFT
                     RAIAEHSPSRAESGLPENGFVFCTFNNLSKITPETFDVWMRLLENVPDSVLWLSQANP
                     SAIRNLRREAQARGIAGNRILFAPFVQAPEEHLARLRLADLFLDTRPYNAHATACDAL
                     WAGVPMVTQIGSSFAGRVGASLLTAIGLPELIAHSEEEYEALALRLARDPTALALLKE
                     KLANNRMTHPLFDTARFTRDLESAYLAMWERHRRGESPRGFRSSETIAQ"
     gene            24741..26840
                     /locus_tag="VGM72_04775"
     CDS             24741..26840
                     /locus_tag="VGM72_04775"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4264609.1"
                     /translation="MSGGTIQKAFGLHRAGKLDEAAKVYSEILRSEPRQVDALYLLGS
                     VHFQRGEFSDALARFDEALAIKPRFIEAMAGRGAALSNFGRQEEALTAYETVLAASPD
                     HAQSWNNRGNTLLVLGREQDALASYDRALSLVPNYVDAWRNRATALLRTGRIEEALAN
                     FERALELKPDFAVAWEDYATVLARLGRREDAIVAYDRALALTPDNPDLLYNRGNLHAI
                     LRRYDEAIHDCEATLARAPDYPYARGVLIHSKLQSCNWRGLDAEIWNISMALAAGKRV
                     VSPFNLKALSDSPPEHLRVAQIWTANEAPAPPFLASKPLGQSPRDTHDRIRLAYVSGD
                     FNSSAVATLMAGVFEHHDRRMFETIAVSFGPAASTPTRVRLESAFERFVDMRGRSDGE
                     IANLLRDMEADIAVDLMGYTGECRTAIFAQRPAPVQVNYLGFPGTMGASYMDYIIADS
                     TVVPEDQQRHYAEAVAYLPHCYLPADRARGVAETIPSRAEAGLPQDGFVFVSFNNSYK
                     FNPVMFGVWMRLLRAVEGSVLWLPETNLAARRNLVREAEARAVAPARIVFAPLVPDAG
                     EHLARLSLGDLFLDTLPYNAHTTASDALWAGVPVLTQIGNSFAGRVAASALKAVGLAE
                     LIARTPDEYEAIALALARDPVRLGEIRAKLGRNRLTTPLFDTAGFTRDLETVFTTMWA
                     RHRRGEMPASFAVAATD"
     gene            26863..29562
                     /locus_tag="VGM72_04780"
     CDS             26863..29562
                     /locus_tag="VGM72_04780"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4264610.1"
                     /translation="MNDHILQNAWRLHQAGQFAEAARLYNQVLSANPRHLGALQMLGY
                     LHFQRGELNDADRIMARALKLDPTSVDALYNRGCVLQALGKERDALASFDRALALKPD
                     YAPAMANRGNVLARLGRFADALASYNLALALMPASAELLLNRGNALFELGRHAEALTS
                     YDQALAKEPQVPIVWHNRGNAQAELGRHVEALASFDRAIQLDARYGDAYFGRGAALVK
                     LERDSEALADFEKTLALDPAHLAALHNRGRILRKTLRIAEALAAFEAILAFEPTYTEA
                     MIDKGITLIALKRHAEALAAFDSALTLAPGNVEALTNRASALIRLKRYEEARADSVRA
                     TAAEPANAAAWHNLGGALSGLKRPKDALVCLDKALALAPDNGASWNNRGAAMVVLKQE
                     EAALPYFDKALQLDPSDVDSWANRARAHSNLRHFTEAIADSDRALAIDPDLAAAKRIG
                     IHARLHACAWERRDDDKRRITAGLTAGERIITALDHRGLCDSESENLIVARLWAMEEY
                     PPAPNPLWRGERYSHDKIRIAYVSTDFRAHAVAFLIVGSFEHHDKQRFETTAISIHPG
                     DGSETRNRIETAFDRFIDATAMSDAEVAARLKEMEIDIAIDLNGYTGDARTGIFAHRA
                     APVQVNYLGYPGTMAAPYMDYIIADPITIPAEHRTYYSEKVVYLPNAYQANDRKRRIA
                     ERTPTRAECGLPQEGFVFACFNNTHKIGPEIFGIWMRLLRDYAGSVLWLFEDNAVAAQ
                     NLRREAAARGVAPERLVFAPRMMPPEHLARTRLADLFLDTLPYNAHTTASDALWMGLP
                     VVTCPGTTFPSRVAASLLHAIGMPELVTSSLAEYEELVRVLAKNPERLAEIRAKLARN
                     RDIEPMFDTAGFTRDLEAAYTGMWERQQAGRAAESFAVPAEAP"
     gene            29563..31245
                     /locus_tag="VGM72_04785"
     CDS             29563..31245
                     /locus_tag="VGM72_04785"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264611.1"
                     /translation="MGDGPPPPEHLAKLHFQEGRFELALRHLDLAIAAAPGTAQLWNS
                     RGAVLATMTRFEDAHQSFSRALMLAPDFASASINRAHVLMALHRYEAAIADYEHVLRI
                     NDRIPFARGNLIRAKLQCCDWRGLAEEWERALDEMRAGRPIISPMVATTLCDAPQDQL
                     RVSRILAETRYPPARTPLWNGEIYRHPRIRIAYLSSDFHAHATATLMAGLFEAHDKTR
                     FETFAVSFGPEDASPMRQRLVHAFEHFLTVGDKNDTEIAKLLREHEIDIAIDLKGFTD
                     QSRPGILTARPAPLQVNYLGFPATMGAPYMDYIVADRVVVPPANAIHYTEKIVYLPHS
                     YQPNDRTRAAGTPPTRASENLPEIGFVFCCFNASYKIQPVIFDIWMRLIRDVPSSVLW
                     LLDDNPQAQRNLKREAAARGVDADRLVFAPRKPVAEHLARHSLADIFLDTLPYNAHTT
                     ASDALWMGLPLVTCPGTTFPARVSASLLSAMGLPELMTSSLAEYEELARALARDPQRL
                     AAIRAKLARSCETSPLFDAAQYARNLESAYMTMWERQQRGDAPATFTVDTAG"
     gene            complement(31255..33996)
                     /locus_tag="VGM72_04790"
     CDS             complement(31255..33996)
                     /locus_tag="VGM72_04790"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY4264612.1"
                     /translation="MMGNSGVRNARKFGFAGAALGGLALAVIGIANFVSIPPAPAAQA
                     RAAATADEPGVLRATLANGLRIILVRNTLAPVVATSVNYLVGSNDAPDGFPGTAHALE
                     HVMFRGSPGLTADQLANIGSIMGGNFNANTRENLTQYLFTVPSEDIDVALRIEATRMR
                     GLDADQKDWNQERGAIEQEVAQDVSSPNYLMFQKLREIMFAGTPYEHDALGTRPSFEK
                     TTAADLKAFYAKWYAPNNAILVVVGNLDPQATLAKIRTLFESIPKKTLGSHPAINPKA
                     LVAVDPISIPTDNANGAAVLAMRMPGLDSADFPALEVLSDVLNSERGDLFALVPAGKA
                     LAAGFSLDPLPKVGMAYASISYPADADAKAAQNELRTTLQKILRDGVPAELVDAAKIQ
                     EERQAEFQKNSIDGLASIWAEAVAVYGLRSPDEDLERIKKVTVADVNRVARKYLDLNH
                     AITAVMVPRGSGRPVASGGFGGQESIALGEAQATSLPDWAEAALNRLSVPASGVHPTV
                     STLPNGLTLIVQPEDVSDTVTVYGHIKTRPEVEVPQGKEGLSQVLGELFDYGSQSLDR
                     IAFQEALDEIGAQESAGTDFTVQVLTRDLDRGVALLADNELHPALPQAAMDVIKRQVA
                     RVVAAQQKSPGYLMQRSLREALFPANDPSLRDATPETVGALTLDDIKAYYKKSVRPDL
                     TTIVVVGRITPEAARAAVQKYFGAWTAEGPKPAIDLPVAPPNKAKLVAVPDESRVQNS
                     VILAQTMAITRSHPDYYALELGSAVLGGSFYSTRLSIDLRKNAGLVYSVGSDLNAGRT
                     RGNYFVQYASDPQNVSKAHQIIVQELKKMQDEPVSTDELKRVKALMLRQIPLGESSIG
                     GIARGLAGRWDLDLPLDEPTEAARRYIALGPAEIQAAFKKWMRPDDLVRITQGPPPQ"
     gene            complement(34099..34881)
                     /gene="modA"
                     /locus_tag="VGM72_04795"
     CDS             complement(34099..34881)
                     /gene="modA"
                     /locus_tag="VGM72_04795"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY4264613.1"
                     /translation="MFPVRWLKTAASAILLLLLSFAAEAQTRDRITVLAAASLTDVMQ
                     QIGKDYQAATGKWAEFSFAGSMTLAKQIEASAGADMFISADSESMDYLDRKNLIARTT
                     RTNLLANSLVLIAPAGSKTTLRIAPGFKLADALMGGRLALADIASVPAGRYGKAALTT
                     LGVWDSVKDHLAQGEDVRATLAYVARGEAPLGIVYATDARVEPKVRVAGTFPENTHEP
                     IVYPVALTKDAKPDAATFLAYLKSGKARPVLERAGFRVLTGK"
     gene            34980..35363
                     /locus_tag="VGM72_04800"
     CDS             34980..35363
                     /locus_tag="VGM72_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY4264614.1"
                     /translation="MTRLSIRIDFEGAEAFGPGKVRLLELIDEQGSIRGAAAAMSMSY
                     RHAWLLLQAVEDTFGAPVISTATGGAKGGGAKLTELGKTVVARYRAIEAQAAQAAASE
                     LTELSKAAAGAVPEERNKRRERSPR"
     gene            complement(35365..35571)
                     /locus_tag="VGM72_04805"
     CDS             complement(35365..35571)
                     /locus_tag="VGM72_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005378494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEY4264615.1"
                     /translation="MKISARNQLKGTIIEVHKGQTTAHVRIDLGNGAHITSSITNEAV
                     DELGLAAGDKVTAIIKASDVMVGK"
     gene            35683..37896
                     /locus_tag="VGM72_04810"
     CDS             35683..37896
                     /locus_tag="VGM72_04810"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY4264616.1"
                     /translation="MAFGKYGGAARFFCRFAAAGLRSSRRYSLRERLAMLPVSGAPVS
                     HPVSIRWDEHQIPFIEAQTDADLATALGIVHAHLRLGQMELMRFIARGRVAEVTGHIG
                     LPIDRLLRTFDIGRAVPDILVGLPHETRNWLEAFVRGINHCAAHAPALPREFALLGLK
                     RELWDLRDIVLLGRLVAADVNWIVWLRLFKFRNLPGWPEVWRHLLRHDTLSFESGSDA
                     GDLGRSFAGAAIRSGSNSYAVAAARSESGGALIANDPHLSFMLPNAFLLAGFQSPSYH
                     AVGMMVPGIPFIGIGRNPAIAWGGASLHAASSDLVAVPKGASLRSREERIAVRGEKDT
                     ILHIRESAWGPVISDLPALRSQETLALRWMGHRSSDEFTAMLRVNRARNWEEFHTAFD
                     GYALPGLEMNCTEVSGDIGQLMAAKLPRRRNAESDDMVSQPDNGWDTPMTSCDLPCRY
                     QPVEGFLASANARPDNCGAIVGFHFSPPNRVRRLRELLGGHRILSLSALMTIQCDCHL
                     SESLTQRDQILSWLQGAGHGAREKQFRRALRNWNGDYDACSHGAAAFELLFVHLARAM
                     VSVTRRAAYDAAWGTRALIWADIMAAPAETRADALGRAVVHAARDFSKASEWGARHRL
                     RLAHPFGMIPLLGRRYRLADIPASGTSETLMTTAHGLTNKRHHARYGSVARHISDLSD
                     PDANHFALLGGQDGWFGSINFSDQMTLWRRGKYVRLPLTPAAVRERFTHLTVLAS"
     gene            37893..39425
                     /locus_tag="VGM72_04815"
     CDS             37893..39425
                     /locus_tag="VGM72_04815"
                     /inference="COORDINATES: protein motif:HMM:NF015291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HEY4264617.1"
                     /translation="MIDATPLLRLYARARASELDHQDAEDAQRGQLLRLLRHAANTRF
                     GIDHGFSAINGITDFQASVKLRRYEDMWRDYWQKPFPRLVDCSWPKTIPYFALSSGTT
                     SGTTKYIPYTEDMHRANSRGAKDMLVHHLRNRPASHLLGGKNFMLGGSTDLTKLAPGI
                     EAGDLSGIVAARFSRWTRGYIYPPRALALIADWDTKIDALSRGILDEDIRSISGTPSW
                     LLIFFERLFALRPEREPKFHAFFPNLELIVHGGVNFAPYRAQFDTLLQGTHAETREVY
                     AASEGFIASADRGPGEGMRLVTDNGLFFEFVPVEELASENPARHWVANFELGVDYAIV
                     VTSCAGLFAYVVGDIVKFVDRSPPRLLVTGRTSYTLSAFGEHLTGDEIEDAAGIAANA
                     IGAAIADFSVGAVFPDAHHSRGVHLYIVEFSGLSPNIDAARHFIDTIDSVLSRRNDDY
                     RVHRSGGFGLDPPRLLSVRRGTFAAWMKSRGQLGGQHKVPRIIANPELFQSLRDFVEN
                     AL"
     gene            complement(39432..41045)
                     /locus_tag="VGM72_04820"
     CDS             complement(39432..41045)
                     /locus_tag="VGM72_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY4264618.1"
                     /translation="MLSIDNVTVRIAGREIIAGATANLPPGRRVGLVGRNGAGKSTLL
                     KVILGQLAPDGGEIAWPREWKVGAVAQEAPGGADSLLDTVLAADAERSALLLAAETET
                     EPHALGYIYTRLDDIDAYSAPARAASILAGLGFSAEEQLRPCSEFSGGWRMRVALAAI
                     LFASPDLLLLDEPTNYLDLEGVLWLEDFLRRYRGTVLIVSHDRDLLNTAAEFILHLER
                     GKLTLYTGGYDVFVATRTAKRALDAAFAKKQEAAREHMQAFVDRFKAKASKARQAQSR
                     MKMLAKMQTIELPVDEHVAPIRIPKPVEASPPLITMHNASVGYEAGKPILTHMSMRFD
                     PDDRIALLGKNGNGKSTLAKLLADKLPLMDGELVRARKWVPGYFAQHQLEELDGALTP
                     IETLAHLRRNLRMEQVRAQLGGFGFSADKATTKVANLSGGERARLMLALATLDKPNML
                     ILDEPTNHLDIDARSELLNALNHFDGAVILVSHDRRLLEATADSFLLVADGRVEPFDG
                     DLDDYRRYLLSGENTPTRRAKSTKPVRAA"
     gene            complement(41179..42354)
                     /locus_tag="VGM72_04825"
     CDS             complement(41179..42354)
                     /locus_tag="VGM72_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEY4264619.1"
                     /translation="MTSDFQADIAAINQIAAVPSILNVACRLTGMGFAAVARVTEDRW
                     IACSVKDDIQFGLKPGDELQIETTLCNEIRQHKEPIVIDNVATDDVYCTHHTPAKYGF
                     LSYISMPIMLRDGRFFGTLCAIDPRPRLLKTPEIVGTFKLFAELIGFHLDAIETLASS
                     DANLRGERRQAELREQFIAVLGHDLRNPLAAIDAGAKMLLRIPLDEKAVNIVRLVQNS
                     VSRMSGLIDNVLDLARARLGGGFALKRDSSEPLEPVLAQVVGELRAVWPDRVVETAFD
                     LNRPVTCDRTRVAQLLSNLVGNALSYGTPGSPVVVRGTTGGGMFELSVSNAGEPIPPA
                     AMERLFEPFSRGHVRPNQKGLGLGLYIASEIARAHGGTLTAHSSPGETRFTFRMPLD"
     gene            complement(42593..43195)
                     /locus_tag="VGM72_04830"
     CDS             complement(42593..43195)
                     /locus_tag="VGM72_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264620.1"
                     /translation="MWSRVCAFAALALCCAVPAMTQNVPVRIYGPIVSVEGRSLTVTN
                     RDGHTISFDLAPQGRIVSNEPLDVRTIKAGDVIAIDEEMGKPIQAHTQAFAHGANAPE
                     QRKMVTNPNATRYLGVVTAAEPTANGVKVTVDLQKNGGKMQVELSNNIVHYRNETESM
                     AEVKPGQFVMANANRNDSGKLVSGFTTIEKNFHRPIDIGD"
     gene            complement(43462..44349)
                     /locus_tag="VGM72_04835"
     CDS             complement(43462..44349)
                     /locus_tag="VGM72_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264621.1"
                     /translation="MSKFASMLFGVGTAVLAVSLVAGAAAQTPSAIPNFAADGRSWRT
                     RGGEIFIPVPGAPQPTGNDTTHPYIHNVDAQRLGTQPTFRISDLTNPNIKQWAKDIMK
                     KDNEEVLAGKIAYTPGSSCRPYGVPAIWNSGGPFFIIQSPKEVLIANQGEQFARHVYL
                     NVPHSTNLKPTYYGESVGHYEGDTLVVDTTGMNDKTFADNFRTPHTEKLHVIERIRLI
                     EGGKKLEIHVTAEDPGTFEKPWQGIRRYDPYHEDRGEVEEGHVGMAEDICQEGNFVGA
                     RNNLASTDYHVPTADKPDF"
     gene            complement(44529..47828)
                     /locus_tag="VGM72_04840"
     CDS             complement(44529..47828)
                     /locus_tag="VGM72_04840"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4264622.1"
                     /translation="MDPRVSKIASLLGSASLLAIASSGAAYAQAQTQTSQAGPADAPE
                     QVLVTGSLIRGAASVGVPVTNVGPQDFVVSGALTTADLFKTIPAFVVTPGAVGTNNGG
                     NIARENSVNLRGLDSVGGYPVRSLMMVDGYRVPPQGEHSGQRDPSIIPSLAIDRVDIL
                     ANGASATYGSDAVSGVMNILLKRGYDGAITQLRATVADGKTQYQASQLWGRTWDGGDV
                     TLTYEWSDSSPLKGKDRSNFTPDFSPWGLDNRTPIASSIPATISVGVPAPSSLRGLGT
                     GCTNCFALPAGAGANFNSSLNGGLGPLAPSSGPGLINWANVGTSANGGATNTAAGTAN
                     EINPYSIGWYDAAQQHNAAVLTVDQRLTKDISFYGEGYYSNRRAEFLNLANQSPASTQ
                     DLQVQVPTRNPYYPTGGAPNNLVVNYNIGLEVPPLTTASELADRYLLGLNFDLPGDWQ
                     GRIYYSETFNGTRTDTHAVNPNAVSAALGWTIPAGFAAGTAPAIGTWTKPATLPYLNL
                     FCDPRSLDCNAQSTLDYVTGRRLSTTSFWFNEKGVNFDGPLFSLPGGDVKAAIGATYT
                     SERWVYSQLDNTKVASLLTPLLQESKGTQFWAIFAQLNIPIVGEANSIPLINALEIEA
                     SWRHDQYSDVGGTSNPKIGFNWTISRDMGLFVRGDWGTSFRAPSFAEQSAIVGNIISG
                     INIPNFVSNRTVSLDCSAGPGSLTDRLLNPGAGYVGWAGVVGNAGTAGTICGARANPS
                     GLGTYSTGGVALKDGFRAYVNTAQSALRPETATNYTLTGEFAPTNFLKGLDLQVTWYQ
                     VKISNALTQFGNPSNSTVNNPALGFSYIVPTDIAKAGIDVAGCSNNNKPAACPEFERM
                     VSAVLNDPQNAVPPGSLTSILWINDGAIGNFGWIKLEGIDFSASYDYDAGDLGAWNAG
                     ITGTYYLHEYEVNNPTPELPNPAIVDDFHTDAGTVGGVAQAGVTTSPRMRYRGRVGWS
                     NGPWSATGFVNYTGHYYNTQNAPPNVNLQCLTAGGTVGGGALPCAISNYTGLEPSYYT
                     FDFSIGYTTGDLPQNDYLKNIGIQFVVQNLMNKLPSFEYQIAAGSAPAAFDVTKSDLG
                     RTFGLILTKAW"
     gene            48182..49063
                     /locus_tag="VGM72_04845"
     CDS             48182..49063
                     /locus_tag="VGM72_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264623.1"
                     /translation="MKRQALFFSIVTAATLALPAFAADEGITGVQWGRNSKDFDGMPS
                     GPKPLRNLNRDANGVADDHALVGDYRNPILTPYAAAIVKQKGEEALAAGFTTPKEQCR
                     PLAPPFSAAIQFDFQILRKKSGDLTIVYHADNQVRHIRMNGTHPAKVAPTPMGDSIGH
                     WEGDALVVDTVGVKSDKFTMVDRFGIPLTEAMHVVERYRPIDGARAKADLTKYEESEG
                     VVGGRTNGYNPDTKLTGLRLEITMEDPKVFTQPLTGVVTYRRLTFGWREDVCADNPVE
                     HYKDEWIGLPTAGHPDF"
     gene            49266..50063
                     /locus_tag="VGM72_04850"
     CDS             49266..50063
                     /locus_tag="VGM72_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4264624.1"
                     /translation="MSAKVVHVAIASDHYAINARFYESLFGMKPSSKPRPSRSVVVGD
                     GYVGLNCIPRREGRCSGIDHFGIEVDDLEDTIARIHKFDPTLEAVKRPPIRPFAAYSA
                     HDPDCNVFDLSQRNIGVQKDVYAENDGETRPRRISHIAIRTRQAEKCAQFYADVFGLA
                     PQNRQDENFYLSDGRVTLMIIPWKIGDYYGQDPARTGLDHIGFKVESVEQVKKDMEFL
                     IGENPLMHGRPLGYGPEGESRLKLFQKCPLGCFHLTDIEGALIDVGE"
     gene            complement(50247..51167)
                     /locus_tag="VGM72_04855"
     CDS             complement(50247..51167)
                     /locus_tag="VGM72_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4264625.1"
                     /translation="MLPDEMRQLKQLKMESIMRTRTAGLFTVTVLAFFGMPAFAQVPD
                     PGLTVTKLKDNIYMAEGGGGASTIIIGQNGVIVVDAKNRPPDGKQLVDEVGKLTNKPI
                     TTVILTHSDPDHVRGLGGFPKGLNLTVIAHENDKKEIEKDLAAGGLQSPPKEYLPNKI
                     ITQMRESITVEGVKMTLIHIAPAHTSGDLAVYFPDYKLVASGDLIGDGDPTIHLFKNG
                     SSEGWIKFVSALVDLDADTYVRGHAGAGTREQVRQSLANAEAKRARIADLVKQGKSLD
                     DIKQAFGEPLKSANRFQTFTETTYEELTRK"
     gene            complement(51174..52010)
                     /locus_tag="VGM72_04860"
     CDS             complement(51174..52010)
                     /locus_tag="VGM72_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264626.1"
                     /translation="MKPKTSKRVFLLGLVVLLAVSAAAAQSPSPDYPDFSGSWESTNA
                     TGLLDPEAGPGPIGDLAGYSHFGFGVDAAHRRNTSRPWIGDYRSPILTPWAAGILKSE
                     AETAIRGEDPFWAQSNCWPGGPAAVLWADQHYFIQTQRTVYIVYARDHEVRRVYLNRS
                     HTKNPAPSWYGESVGRYEGDTLVIDTIGFNDRSLIDRYGTPHSSDLHLIERYKVLSDG
                     KTLEGTLTYDDPKAFTATWSAITKYRRASDPLAEAACAEAANNPVTGKFFRLPTASKP
                     DF"
     gene            complement(52079..52963)
                     /locus_tag="VGM72_04865"
     CDS             complement(52079..52963)
                     /locus_tag="VGM72_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264627.1"
                     /translation="MKRQALFFNILTAAMFAVPAFAAGDGITGVQWGRNSYNFGGMPS
                     GPQPVKNLSRHLDGVANSSQMVGDYRNPILTPEAAAALKKKGEQAIAEGGFSTSEDQC
                     RTIAPPLTAAVQFDFHILPKKDGDLTIVYHVDNQVRHIRMNGTHPAKLAPSAMGDSIG
                     HWEGDMLVIDTVGIKTDAFISSDLFGTPQSELMHVVERYRLIEGARAKADIAAYEKTE
                     GVIGGRTNGYTPDPNRKGLRLEVTMEDPKVFTQPLTAVVTYLPLTFGWREYVCADNPI
                     EHYKDEWIGLPMANHPDF"
     gene            complement(53208..54095)
                     /locus_tag="VGM72_04870"
     CDS             complement(53208..54095)
                     /locus_tag="VGM72_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264628.1"
                     /translation="MNAQPWNFLLLVILGTFGAASGVGETAPIPDFSGTYNHQGIPGF
                     EPLASGSTSLVNLHRRERNVSNNRQLVGDYRNPILRPETAALVKTFGEMSLDHYGYPT
                     PRNQCWPGGVPFEFTNNEIQMLQTPTEILILYNGDHQIRHVRMNQSHPARVTPSWYGD
                     SVGHYEGDTLVIDTVGVKVGPYAMVDWYGTPHSPALHVIERYRLLDYAAAKEGLERSA
                     KENVIPDFDGKFNETSQYLQLRFTVEDPVVFTTPWSATITYERVNPQIWGEDVCAENP
                     RKYGTEKDVAVPTAGTPDF"
     gene            complement(54129..55847)
                     /locus_tag="VGM72_04875"
     CDS             complement(54129..55847)
                     /locus_tag="VGM72_04875"
                     /inference="COORDINATES: protein motif:HMM:NF006203.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEY4264629.1"
                     /translation="MSIKIIESGTVADAYLALLADRGIDCLFANAGTDFAPIIEAYAK
                     AQVEGTKVPKPITVPHENVAIAMAQGYYLKTGRPQAVMVHVNVGTANALCGIINGWRG
                     NIPVLFTAGRTPYSEEGGLMGMRGGEIHWPQEMRDQRAMVREFVKWDYELPNGETMEA
                     AVDRALNIAMSEPMGPVYLTLPRETLAAPIKNFRYASPSRHATPSPAFPDKNAIDRAA
                     DMLAAAEFPLIITRAAGRVETDVAKLAAVAERFAIPVFERKHHYMSMPWNSPMHLGSN
                     PESYFDAADVIVVLEADVPWVPKRKAPRPDCKIIHIGADPLFENYPLRGFPCDLAITG
                     VLGASLPALTDALATREKAASSRIEARRCKVAEARDTLHTRIAANLEKAKNASTIHPG
                     WINHCIDRVKGEDGIVVKEALTPSEHLHFNKPGTFYSLGQGGALGWGLGTALGMKAAM
                     PDRLVICAVGDGSYMFGNPIAAHYVGKAEKLPTLTVIYNNEMWNAVRRNTRDVYPDGY
                     AAKSNHEPLTYFEPGTRYEKTIETVGGYGERVENAAALPKALDRALDAVAGGQQALLN
                     VICGTG"
     gene            complement(55861..56721)
                     /locus_tag="VGM72_04880"
     CDS             complement(55861..56721)
                     /locus_tag="VGM72_04880"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4264630.1"
                     /translation="MSTDRVTRVQDKFVTVDGLALRYIEEGEGPAVLLLHGASLGSSA
                     DVFIRNLAPLAQAGFRAIAFDQPGFGLSDVPTDLSPAYRRDSIPKFMEALGLKQAALA
                     GHSQAGNPAVQLALTDPSRYSHIVVLGTGSLLPPVDGEGEGNKGAAQQRLERRMAQSE
                     PAIDDTRKLLEANLFHHELITPEELALRHARSIGRNFQAFVARNQAAEVLPPKQPKMP
                     LWQRLIELKMPLLMIFGREDRAKAAERATLLKEKFPQLDVHIVNGCKHLVPWDAADEF
                     IRLAVPFLKN"
     gene            complement(56748..58157)
                     /locus_tag="VGM72_04885"
     CDS             complement(56748..58157)
                     /locus_tag="VGM72_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4264631.1"
                     /translation="MTGTVDVADVIDSHKVSRYQCLIAFMAGLTLFLDGVDNQSIAYI
                     APALSHDWDLARGALRTVFSSGVLGVAVGALVIGPLADRYGRKRVTVLTVIYVALFSL
                     IATQVPEISTLLLPAIPDASNLNVLMGLRFLTGLGLGAAVPLAVVIVNEFAPQARRAA
                     MVTLMGCGYAMGSASGGLLASHLVPAFGWQAQFYAAASMTFVMAGVLWIWLPESIRLL
                     TVQGRSDGVIAILRKIAPELTFEPGTHFVLRQELREQSANQPRLVRLFMEGRTSATVL
                     IWLCLFMNTLALNYLNNWLPTLTTETGLPEAQALRAAASLQFGGILGVITLGFLSDRF
                     GFFKVLAGSFAAGGVFIATIGWAGGAFYPLAATIFASGFFNIGTQITLAALAATLYPT
                     DIRSTGVSWAHGVARVGSIISVMFAGTMLALHWPLQTMYYLVSIPMFFGCVWILILAN
                     VRAKTSRGAAEPQGRRAVA"
     gene            complement(58322..58567)
                     /locus_tag="VGM72_04890"
     CDS             complement(58322..58567)
                     /locus_tag="VGM72_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264632.1"
                     /translation="MLQQNNACVRQFRAAGMTHEKLNLQLLFQLANLEPEGRLRDPQN
                     LCGLCEAFLFAHSNEIHQLPDIHAILMVSQIVFTGGM"
     gene            58535..59410
                     /locus_tag="VGM72_04895"
     CDS             58535..59410
                     /locus_tag="VGM72_04895"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY4264633.1"
                     /translation="MTDAGVILLQHARDILERIELAGAAIRDSTSTQGERIIIGMAPS
                     ISAMLSYPLIKLGAQKLPNVTIVLVEELSSVLLEWLENGRIDIAIGYELLHSAKVAGD
                     PLFVQDFFLVQSPKAKTRRRNLNFAELADIELLMPASPHGLRELMEAGAARLGIHLKI
                     RFEVQSISVMKELVEQGICAAVLSYGAVARECEKGRLVATEIVNPTVKRIAYLAYSLR
                     RPRSKAKDAVLAFIRELALKQKGVNSDLHSMAPSSGRATKDLGAQKKHRRNGVTKPRT
                     VLTSSGGPAFGGRKT"
     gene            59572..61047
                     /locus_tag="VGM72_04900"
     CDS             59572..61047
                     /locus_tag="VGM72_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY4264634.1"
                     /translation="MPAAVLRNEWKPVHARMLIGGEWTISTQRIEVFSPARPDELVGT
                     VPRGTPADVERAIAAAKAAQPVWASKSYSERARIIAPLLERLADDIDERTVLYVRENG
                     KTTPEARGELAGVPSRQRLTLDLAAELDQRREADAPNGKSFVGYLPFGVVVSIVPWNA
                     PMGLAFLQIIPALLAGNTVVLKPPETCPLSLIRAIEMIAPMLPPGVINVVTGLPTEIG
                     DTLTTHPDVDKVAFTGSVAAARKIMANAALTIKGITLELGGNDPAILLEDADLGDAAM
                     GRMANSVYRMTGQVCMAIKRIYVPESIKGRFLDAFTRTVDKIVAGDGLDTTVTMGPMH
                     TARALERAQAIVADAAKRGAKVQILGQVPDKAAFGHGHFMQPTVVSDIAEDAPLVVEE
                     QFCPAVPVATYRDVDDAIARANATIYGFGGSIWSRDIARATDLARQLHAGTVFVNTHG
                     TESVNRKLPYGGIKHSGIGRRAGIEGVREYMQVQTLTTFEQ"
     gene            61049..63508
                     /locus_tag="VGM72_04905"
     CDS             61049..63508
                     /locus_tag="VGM72_04905"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4264635.1"
                     /translation="MHAQTPLEIAIGEERDTAADSDTHETAQLLAEAIRLHRSGRMAD
                     AARLCERVRQHAPDNLTALLLSGTIELQSGRHHAADQYFQLAQAAAPHSVDAATARSN
                     TLRAIGKHDQALAVLEDILRHSPDHAIAWNNRGNLLLEMGRNTNAIESYDRAISLHPN
                     YLDALHNRGVARMASGDNFAAEADLTRALELKPDYVPALVNRGTARVQQSPERAHDAI
                     TDLRQAVLLERTNPDAWHMLGRSYLALGQHAEALSNWSTGLARHANHAAMRHDRAFLF
                     NALGRYSDAATDFERLIALNLHDAAAWQGRGIALARLNHEKEALESFSQALRFRPDDV
                     VSLYNRAAIFSRLKRYEEAASDLEVLVAADPEFPLARGLLLSARLYLSDWKGLERQRE
                     AIDACVRRGSRVIHPFAHLLISDCSAAQLACARRHTSGAYPTARVPLYRGEQYSHEKI
                     RVAYFSGDFYQHAVPSLIAGVLENHDSSRFEILGISYGGNDKSPMRARLEKACARFFD
                     VRERDDASIATLLRNLEVDMIIDLKGHTGPERPGILAHRPCPVQVNYLGYPGTMGADY
                     MDYLIADRIVIPPEQRQFYSEQIVYLPDTYQANDSARPIAPDGITRGDAGLPEAAFVF
                     CCFNGSQKILPPVFDSWMRILSHSENSVLWLLEDNQGTAVNLRREAQARGIAAERLIF
                     ARHERPDRHLARLKLADLVVDTLPYGAHTTASDALWAGVPVLTCIGSSFAGRVAASLL
                     TAAGLPELIMQSVVEYEATAQNFAATPGLLLKIREKLARNRESCALFDTVRMTRNLEA
                     AYVKMWQRHQRGESPTGFALP"
     gene            63684..64448
                     /locus_tag="VGM72_04910"
     CDS             63684..64448
                     /locus_tag="VGM72_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264636.1"
                     /translation="MEEFRVHARAGVAIAAALAFSAIAGSAYGAGATPDISGTYWTPR
                     YYATLPIVGGGALPLTAMGKEAYAKNQAGLKSGEITDAARKFCVPDGMPRAMASPYPF
                     EIIQDPANQITMVHELNHQIRVIMMDKPLQTYEQVVAFPYYNGHSVGHWEGDTLVVQT
                     NGFNEKTFLDATGAPHSDMLVTTERIRKTGANQLEDVITIHDPDYYTRDFQARFTYVL
                     RNDVHLEDYVCGEPHRNIDMIPGIPEARRANAAAGR"
     gene            64694..65098
                     /locus_tag="VGM72_04915"
     CDS             64694..65098
                     /locus_tag="VGM72_04915"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HEY4264637.1"
                     /translation="MKFKALGLAAAALGLYAIPALAHHSFAMFDADKKIALEGTVKEF
                     QWTNPHSWILIMVPNAQGTPEQWAIEMGAPGGLARQGWVPKTLTPGMKVTATIHPLKD
                     GTHGGQFMAVALPDGTLKGNPNGAAGANAGAN"
BASE COUNT        12621 a        19969 c        19653 g        12870 t
ORIGIN      
        1 atgcgatgcg cacaatagga cagatcgtcc agcatctgcc tgtcgtatct ttccgcgaac
       61 tggtcgaaga gatggcgcac atagggcgca ggcgaacgct tcgcctggag catcgcacgt
      121 ccttccgcga ttttcgcagc cgcgcgttcc gcgtatgggg attgtatatc ggagagcgat
      181 gacaatatct caatcgcatg ttcagcttct ccggcatcga gccaggcaca gcccagggcg
      241 taccgcgccg cctcggatgc cggagcaaga ctcgctgcgc gttggaaccg ggcgatggtg
      301 gccggcaggc cgcactcttt tggcgatttg cgtttcatgt gaatcacacc gagggcttat
      361 gtcgcgaaag agatcgaaat ttccttggcg cgcctttaac ggtgcagcgg gcgcaagcca
      421 aggctccgct gcctcttgag ctatccctat gagccacatg agggtttccg gtgcccgtcg
      481 agacattgag accgcattgc ccaaaatgca aaagcaagcc cgcgcaaggc cagaggaaac
      541 tatccgaaac cgaacgcgac aggctcatcg gccctgcgtt caaagccgcg tcggcgcgct
      601 ttgcgaccgc ctatgtttgc aaattctgcg acgccatctc atcaggatgg gttcagcagt
      661 tatatcagac gtctgcgcag accttaaccc ggcgcaagaa tgtgccccgc cgccagcgcc
      721 ggccccgcgg tccaggaatc ggacaggggc acgccatgtt tttcgatttc aagcatggca
      781 tgggcatagc cctgcgcaat cgcctgctcg aagaatttcc agtcgcgcat gccgacacct
      841 tcgagcgggg gttcgtaaag gaaatcggcc tgtgcgcgaa cgctgcgccg ctgcagatcg
      901 ctgcccaccg tgcctgcacg catcaggatg gagatgatcg agggcgaacc gcgcatacgc
      961 tgaccgatga gcgaaaaaat cgaccgttcg ccatagcgtg cgtcctcggc atgcagatcg
     1021 atctcacccg cgacatcgac cgcgatgatc gggccgcgcg cttcggcggc catgacatcc
     1081 accggcagat tgttcatgac gccgccatcg accagcagat ggccgccttc ggtgaccggc
     1141 ggcaggatgc ccggcaaggc tacgctggca cgcaatgccc gccacaatag cccgctgcga
     1201 tgcacataat cacgtcccga ggtcagatcg gacgagacac agaaataggg tttggcgaga
     1261 tcctcgatcc ggatgtcgcc aaagtgcttt tgcagcagcg cgctgacctt ggctccgcgg
     1321 aacagcgcga tcagcggaac ggtgaaatcc gaaagcggct tgttcacgac gaaagcgtcg
     1381 cgcacgcgtt cggacaattc atcgatgtcc cattccatgg cgattccggc agcgacgata
     1441 ccgcccatgc tggtgccgcc cacataatcg aatggaacac ccgcttcctt cagcgccttc
     1501 agcacgccga tatgggcgaa gccgcgtgcc cctccgccgg ccagcaccac gctgaccgcc
     1561 gaaccggcga tgaagcgcgc aagccgcttc atatcggaca tattgccggc gcgcagatga
     1621 tggtgcgtcc cgaaaagatc gctccgcaac tcgatatgct ccggaacccc gcgtcttgag
     1681 gcgtcgctgg catggaccag cagcagttcg ggaggattcg atgtctcccg cttgaaataa
     1741 cgccgctcga agggatggag cggcaggttt ctgtcggcgc gcgcaatcag aagcacgcga
     1801 tcggcttggc gcaggcagaa ttgcgaccac gtgctttccg gctgatcgcc ctggtagaac
     1861 acgacatcgt gctcggcttc aaagcggttg aaccaatcgg tgggctgctc gctcatggat
     1921 ttgtccagca ccgcggcctt ggcgcccatc tcttccagga tggccgcaat gctgcgcgca
     1981 atcggatcgt cggccaggcc atgctggagc ggcacaatcg cgaacgtctt gggccgcgtg
     2041 cgctggtggc cgccacgcgt cgtttcgcgc aggcgccgga cgacgacctt cagcaggttc
     2101 agcatcacgc gcggatagcg cgtcagcagc atctcgaacg ccttgggccc gagccgcaac
     2161 aattcggtgt cccgcagggc gatcagggtc gcggaatgcg attctccggt gagcagcgac
     2221 atctccccca ccgtctcacc ggcggggatc gttgcgacga gacgtttggg ggcattgtcc
     2281 tcgtcgacga aaacgccaag cgacccagtg accaccaaga acacggcgcg gtcgttttcg
     2341 ccgtcccggg gcagttgagt gccaccggga agaccgaacc aatcggcttc ggcaagcaac
     2401 cgcctgagcg cgtgatcccc cacatcggcc aggatgctga agctcctcag ccgctgaggc
     2461 agcgaatccg gaaataccgg atcgccatag ctgcggaagg acgagaacag cgtcatatcg
     2521 agcgctcggg aaatgtcgaa tttcatacta ccaagccgtc catcccccgt ccgccgccaa
     2581 taatgccccg gtgacgaaac tcgatgccgg gcttgccagg aacaggaccg gaccagcgat
     2641 ttccgatggt ttgccgattc ggttgagcat ggtgcgcgcc gccagcctcg cggcaaaggc
     2701 gggatcggcc tccagcacgg cctcgcgcgg gaaaggccca ggcgcgaccg cattgacccg
     2761 gatacccgag ggaccgagtt ccgccgcaag atgacgggtc agttggacca gccccgcttt
     2821 ggccgcacca tagtggggcg ggctcatctg cccgggtgcg ctgtagagct tggggtcagg
     2881 gctgaccgac gcatacatgg tcgcgatgtt gacgatgctg gcgtcccccc gttcggcagc
     2941 cgcacgggtg agcgccggac acgccgcgcg cgaagcctcg aaggcggccg tcacggcgct
     3001 ccgataggcc gtgtcgaagt cctcgggcgt tacctgggcg agagctttcg ggctcatggt
     3061 gacggcattg ttgatcaaga tatccaaaca tcgctgcgcg gcgaaaaagc ttcgcacggt
     3121 ctcgaaatcc atcatgtcaa acgccgcaca ctcggtggcg atcccgtcgt tctcaagttc
     3181 gcacgcaaac gcggccagcc gctccgcgtt ccggccgttg atgatgagct tcgcgccggc
     3241 ttggcccagc gcgagggcca tggcccggcc cagatgcccg gcggcgcccg aaaggaacgc
     3301 cacccggcca tcgagccgaa agagagcttg cggattcatt gcggcgtcca ttgggcggga
     3361 tcgaggaggc tttccggcac gcgcggggcg cgcgctgcga tgcgggcgca atcttcgggc
     3421 gcgaggacgg ggcggtcgca gagcctcaga ttgccgtcga gttgagcctc ggtctcctgg
     3481 ccgatgacca cgccgtcgat ccaatcctgt ccccgtgcat aggcaagggc aagatcggca
     3541 acgccctccc ggccaaaggc gcgcgcggtt tcctccagcc aggcgataag ccgcgggcca
     3601 tccacgccgg gaacgcgctg ccacaggcga gggtccccgg caatcagaac accctgcagg
     3661 aatatgctgc gggcatgaac ggtcacttcg gggcgggatc gcaatgccgc gatcacgccc
     3721 gcgtcgcgcc agcgccaatc gagcagattg aacggaagct ggatgtgcgt gacgctcgct
     3781 tcgcgcaggg cctcatgcgc ctcggcagga gattgcacgg agacgccgag cgcagcgatc
     3841 acgccgctcg cgagatggtc cttcaccctg gcccagacca cgccccgata ggcggtgatg
     3901 tgacgggcgc gatgaagcag cagacaatcg agagaggtgc ggcgcagcgc ggccagggat
     3961 tgcccgatgc ttgcgtccac ggccgccatg acggcttcgc gcgaagcatc ggccgcgaga
     4021 tcgccgagcg cggagagctt cgtgatggtc cgcacgctcc gccggccatc cagcgcctcg
     4081 ccaagccgtt cttcggcatc gccataggcg cgcgcggtat cgaaggtgac gacgccgcga
     4141 tcggccgcac gccggacgag ccggagggcg gcttcgcgcg ccggcttgcc ggtgcggttc
     4201 gcagcaccat aatcgaggcc aatctgcgcc gaacccatca cgagttcggc ccggcgggca
     4261 atgcccatgc tctcctcgcc accacaatcg gaaaaaggca agaatcctct atcgtggtaa
     4321 aggacgcgtg aacggcggcg gtgacaggga ccccaacccg ctctaagctg gaaaacacca
     4381 tgaagcgcct tattattctg attctggcgc ttgcgatcgg cgcctgcgcg acgcggcctc
     4441 cgggtccaga gttgccctcg tccgaggctc agatgagcgc gctcaaggag cgcctctttg
     4501 tgcttgtgga agccgaacgc catcggctga acagcgaggc caggccgctg atcctggacc
     4561 cggaattgat gcgtgccgcc caaacccatt ccgacgatat ggcgtcgaaa cactccttcg
     4621 acgccggcaa tccgaacggc aatcttgcaa tcgcgacctt gctcgcagac ccgaaattcc
     4681 gcggcttcgt tggagaaaat tcggccgccc agtatttcac ccccggcgcg cgcatcgatc
     4741 cggacgccta tgcgcgaggg tttctcgaca tctggctcaa gagtaccgac cacaagatga
     4801 acctgattta tcccgctttc gacaagaccg gcatcgggat cgcggtgagc ggcgacatga
     4861 tctatgcggc cgaactgttc gccaccgatc tcggcctgcc ggagccccag tgattgggtc
     4921 cagtgattcg accatgacga acacagcacg catcaagact tatggcgaat tctggccgtt
     4981 ttatctgcgt gagcacgcaa aacccgaaac gcgtctctgg cacatcgtgg gcaccggaac
     5041 cgcgagcgtg ctcgtcgcag ccgccgtttt cagcgtcagt tacgagcttc ttctcgccgc
     5101 catggtcacg ggctatggcc cagcctggtt cgcacatttc ttgatcgaga agaaccgtcc
     5161 cgccacgctg cgctatccca tctggtcgct ggtctccgat ttccgcatgg ctggggcatg
     5221 gctgacgggc gatctcgcac gggaacttcg ggacgcaggg gtcgagggac ccgtcgattg
     5281 actggatcgg gatagcgatt cctgggaata aaggcggttc gcgcgctaag cttgctttac
     5341 acaacatctc gcgccagtta ctttttgttg tgtgggaggc gccgtcagcc acatgaagca
     5401 cacacttcct tggaatgtga caggtatccc gccggaagtc cgggagatcg cgcgcgcagc
     5461 ggccctgcgc gaaggcatca gtgtcggcga atggctgacc cggcggatca cgacggacgc
     5521 aggcaaactg accggcggag agccccaagc tccaatggcg tcccccgtcc gctcgccgcg
     5581 cgaagacctg catccggaca cgtccgatcc tgtgttccgc cggatcgacg aagcattccg
     5641 caatctcgcg caccggctcg aaaacaccga gcgcgcgcaa accgatgcgc atcgcgcgat
     5701 gcgtgcggcc gcacacgaga tcaacgccgc gacacgcgac caagcggaag ctttccagtc
     5761 catcaccacg cgcatcgatc gcgtcgagcg gcagaccgat accggagcgc tgcgtgacgc
     5821 cgtgcgggga ttgcatcagg gcctgtcgcg ccttgccgag caaatcgcca agaccacgaa
     5881 cgaatcttcc aatggaatta ccgcgcttgc cggcaatgtg gaaatgctgg ctggcaggat
     5941 ggcgaatgcg ggcgaagaat cgcaacagaa cgcccgtgca ctcgaagaac gctttgcggt
     6001 actggacgaa cgtataaggg atagcgaagg ccgtctgaag cagacggagg aacgtcttgc
     6061 tgccgctttg cggagtgatg ggaccgtcgc gatgttccaa tcgcgcttgg cgtctaccga
     6121 acagcgcatg cgggaatcgc tcgacaattc cctgtcgggc ttcgaacgca gcctgaaaca
     6181 gatgggcgag cgtctgcaaa agaccgaaat gcacgacggg tccgacaatc gaatcgatga
     6241 ggctctccaa accctcgccg tccgcgtcgg ggccatggag agtaggcaaa gcttcccggt
     6301 cctcgaatcc gctgccggcg acacgctttc cctcatccaa gccgccgtgg acggcgccct
     6361 gccttcaggt gccccggact tggccgaatt ccgtctgacg ccggacgcca gccttgcgcc
     6421 cgcatccaca ccggcagccg ggatgccgcc tttggcacat gagatcgatg cccccgattg
     6481 tgttggcaca atcggagacg cgaagttcca ccgggcggac gaagtgcagc cggcccgcgc
     6541 atcggccgaa aattttcttg cccaggcgcg acgcgctgcc atgcgtgcca cggaacgcag
     6601 cgcctcgcac ggcacgcgtc accgcatcgg cgttcccgcc ggcagcggac ccggactgcg
     6661 ccaacgcatt tcgcagcacg cctccatgac gttcctgttt cttgtgctga tgggggccgg
     6721 gttcctgctg acccgcgctg tccagaatcg cgcggaggtc ctggcattcg atccttcaca
     6781 cctgattgcg ctcgccgcgc ctgcaagcgc cggaccgatg actgcgcccg agaagacatc
     6841 ggctcctgcg attccggcag ccgttccccg gcaagcggtc gaatcggttg cgcctgccct
     6901 tgccgcaccc gccgacagcg tggcctccgc atcggtgctc ccatttgggg cggccggcga
     6961 aacgccgcta gcgcagctcc tggcgaaagc caatagcggc gatcccaaag ccgccatgtc
     7021 gctgggattg gaatatgccg atggcgacgg tgtcgcggcc aacgacacgg aagcctttta
     7081 ttggctgcag aaggccgcgg aagccggcga ggccgtggcg caatatcgtc tcggcaccct
     7141 gtatgagcgc ggccgtgggg tgcccgccga cacgcggcag gctctgcact ggtatggcga
     7201 ggccgccaag cgcggcaacc gcagggcgat gcacaatctc gccatcgtgt atgccgacgg
     7261 ggcagccgcg gacaggaact tcccggaagc gcttcgttgg ttcaaggacg ccgccgaact
     7321 cggcctgacc gattcgcaat tcaacttggc ggtgctctat gagcgcggcc tcggggtgaa
     7381 accatcgctc agcgaagcat acaaatggta cgaaatcgcg gcagcatccg gggacaacca
     7441 atccaaagcg cgcgttgcgg cccttatgaa ccagatcacc cccgaagatc gcgataccgc
     7501 cgacaaggcg gctagggcct atagaccgcg gccgatcgat ttggccgcca atgacggctg
     7561 acgggaccca cacaaatcag atatcagacg ccggaaagcg ataaatcggc gtatcctgat
     7621 tttcgtttcc ttcgcttttg cgattttcgg tctgagcatg gaagtctatc tcccggtcgc
     7681 cgagttgtcg gtcaattggg ccatgatcct gggtatgggg gctgcggtcg gctttctgtc
     7741 gggcatgttc ggcgtggggg gcggcttcct gatgacgccg cttctggttt tctacggaat
     7801 cccgccgggc atcgcggtgg caacgcaggc aagccacatc acggcctcct ctctctccgg
     7861 cgccctcgcc caatggcgcc gtcagggcgt ggacgcgaag atgggactga tgctgctcgc
     7921 cggcggcttg ctgggctccg ccggtggggt ctacgtgttc agcatcctgc agaagctcgg
     7981 ccagatcgag ctggtggtga cgggaagtta tgtgatcctg ctcggctcca tcggcggcct
     8041 catgctcaat gagagcatcc gcaccatacg cgcggcgcgg agtggcaagg cgcagccatt
     8101 cgcgaaaccc agccagcaca gctggattca tgggctgccg ttcaagatgc gcttccggcg
     8161 ttccaagctt tacatcagcg tcattccccc cctggtcctg ggcgtgctga tcggcgtgct
     8221 cactgcgatc atgggggtgg gtggcggctt catcatggtg cctgccatga tctatctttt
     8281 acgcatgccg accaatgtcg tgatcggcac gtcgcagttc cagattcttt tcgtcacggc
     8341 cgcaacgacc atcctgcacg ccaccagcga tcagaccgtg gacatcgtgc tggcgtttct
     8401 tctggtcgtg ggcggtgtcg tcggcgtgca actgggcata cgcgtaggtt cgcaattacg
     8461 cggcgaacaa ttgcgcgcgc ttctggcgat tctcgtggtt gcggtggcgc tcaggcttct
     8521 cgtcgggctg attacgaggc cgggcgacat ttattcgatc atcgcgggga cgccatgatc
     8581 cccaggcgcg ccccggaccg ccaccggcgc gcgtgccccc gcgggtgggg atttgccatg
     8641 gttttcctcg cggcgttctt tcccggcgtc ggcgctaggg cggacgatct cgtctcgggt
     8701 ctttccaccg acctcatcca gatcacatcc aatttcaccg gcacggatat cgtgctcttc
     8761 ggcgccatcg agccaactga ggaagcgggg cccgccaagg atcaggatct cgtcgtcgtc
     8821 atccgagggc cgcaggtcga tatgaccgta cgccgcaagg cacgcgtctt cggaatctgg
     8881 gtgaatcgcg aacaaaccgt gttcacgggt cttccgggtt attactttct cgcgagcacc
     8941 cggccgctcg acgatatcgc ctcgcttgcg acgcagcagc gcttccgact cgggaccgcc
     9001 aatctcgact caccggtgct gggcggtatg aaacccgatg aggcagcggc cttccgcgcc
     9061 gccgccattc gcgaccgcaa gcgcgagcgc ctctattggg aaagcccgac gggaatcgag
     9121 ttcttgagcc ggacgctgtt ccgcgcccgt atcgcggttc cggcctcggt tccgccggga
     9181 gagtatcggg cggaagtgta tcttttccag aatggaaccg tggtgagcgc acaatcctcg
     9241 cccttgtaca tcgacaagag cggcttcgag cggcgggtct acaattacgc ctatcaggca
     9301 tctttcgctt atggatttgc cgcggtgctc atggccttcg ccttcggctg ggcggggttc
     9361 ctggtgttcc gtgagcgata ggacggcgcg ttataatctg tagacggatg ataacggcat
     9421 cgccattttt tatcgagagc gcaacctgag tttatttaat tgaacgcctg gatgcccgtc
     9481 tgggcacggc cgagaacgag tgcatgaatg tcgtgcgtgc cctcataggt attgacggcc
     9541 tccagattca gcacgtgtcg gatgacattg tattcgtcgg ccacgccatt gccgccatgc
     9601 atgtcgcgcg cgatccgcgc gatgtcgagc gccttgccgc aattgttgcg cttcaagagc
     9661 gagatcattt caggcgtcgc cttgtcctga tccatcagcc gcgcgagacg cagggcgcca
     9721 cccaacccca atgcgatttc tgtctgcatg tcggcaagct ttttctggac aagctgattg
     9781 gcggcgagcg gcctgccgaa ctgcttacgg tcgagcgtgt attgccgcgc tgcgtgccag
     9841 caggattcgg ccgcgcccat cgcgccccac acaatgccga agcgcgcctt attgaggcat
     9901 ccaaatgggc ctgcgagacc tttcacctca gggaacatgt tttcctcagg cacgaaacag
     9961 tcttcgagca caatctcgcc cgtgggcgaa caacgcagcg agaacttgcc ctcgatcttg
    10021 ggcgtcgaaa atcctttcgt gccgcgctca acgataaaac cgcggatgcg atcgtcttcg
    10081 gacttcgccc agacgacggc gatatcggcg tacggtgaat tggtgatcca ggtcttggcg
    10141 ccgttcagtc ggaacccgcc gggtgccttg acggcgcgcg tcgacatgga accgggatcg
    10201 gagccgtgat cgggttcggt caacccgaaa catcccagca gttcgccctt ggcgagcttg
    10261 ggaagatatt tcttcttctg ggctggcgtt ccataggcat gaatcggatg catgaccagc
    10321 gcgctctgga cgcttatgag cgagcgataa ccggaatcga cgcgctcgac ttcgcgcgcg
    10381 atcagaccat aggcaacgtt gttgaggccg gcacctcctt cttcttccgg cagcgtggcg
    10441 ccgaggaagc ccatggctcc catcgcctcc atcagcttgc ggtcgaagcg ttcgtgccgg
    10501 aagtcctcgc gcacgcgagg ggcaagctcg ccctcgcaga agcgacgggc ggaatcgcgc
    10561 accatgcgtt cctcgcccga aagttcctgt tcgatgagaa gcgggtcggc ccaatcgaaa
    10621 tccgccatga taatctttcc gttccgtcgc tagttggcaa gaaaggccac aaccgcatcc
    10681 ttgaaactgc gatcgccgac cgtcgaatga tgattgcgct taggaacgat cagggtcgcg
    10741 gcatcgcgga acgcatcgga cagtggcccg ggcgatcccg ccgtgtcgtc ctgttcgccg
    10801 cagacgacaa gcaccttctg cgccagcgcg cgcagttcct cgggcggaaa gatgcgtcgc
    10861 ggacggcgga tgcaagcggc catcgcgaca agatcgtcgc cggctttttc gcagaagcta
    10921 cggtattcgc gcgccacggg atcggtgacc tcagtggcgt ccttcgccaa aagccccgcg
    10981 gcaatggatt caaccgcgtc cttcgaggga cggaaataat tctcgccgat gccggccagg
    11041 atcgtgcgcc gcacccggcc cggcgcatca tgcatcagcc ggatcgcgat ctgcgcgccc
    11101 atggaataac ccatgacgtc gacttccggg atttcaagcg cggccagcac agcgatgaca
    11161 tccatcgcca tacgctcctc atcgtaatcg gccggctcat gcggcttctc gctttcgcca
    11221 tggccgcggc agtcgatggc gatcagtttg tgccgggcgc gctgaaccgt ctgataccaa
    11281 ttggtgttgg accaggtgat attgcgattg gcgccgaacc catggatcag cacgagaggc
    11341 agaccatcgc ccatacaatc ataggcgatg tccgttccgt ccgagacagt aacgtaataa
    11401 gtcatgggtg gggtaactcg cgcgtttgga cgcgctttgc aagccggttg cgctcctgta
    11461 cgcgaaggcc cagcatggct ccatgaatgg atggcttttg gtgggcgccg tgaacgggtt
    11521 ccttgcggtg gcggcgggcg cttttgccgc gcatgggctg gagggccgcg tcagcctgcg
    11581 cgcgctggag atattccaga cgggggcagg ataccacttg acccatgcgc tcgcgatcgt
    11641 ggccacgagt ttcgtcgggg gtgctagggg ggcacgcgcc ggtgtcgcgg cctggctgtt
    11701 caccttgggc atcgtgctct tctcgggatc gctatacttc atggttctgg cggactcgac
    11761 cgcgctcgtg ctggcaacgc cgttgggcgg cgtgtgcctg ctggccgggt gggcagcact
    11821 cggattcgcc gccctcaaac acgactaaga ctttccggct gcgtccaaaa ctgcgattct
    11881 gtgaatctca tgccaccatc caacccgacc gacagctttt cctacgccac cgaagacgat
    11941 ccgcggctga agcgtctgct catccgcgcc atcgagcgca tgaccggaca gccgcacctc
    12001 aagcggctct acgatgagca ccgcaccaac ccggtaccgg gagaaagctt ctgggcagcg
    12061 gcgatccgga atctggaact gaatgtcgtc tgcaatgatg atgccctcgc gcaattgccg
    12121 gctacgggcc cattggtcat tgtggcgaac catcctttcg gcgttctcga tgggctgatc
    12181 atctcctatc tgacgtcccg ggtccgcaac gacttcctcg tcctcaccaa cagcgtcctc
    12241 tatcaggccg atgagatccg cgagcatttg ctgcccatcg attttgccga aaccaaggaa
    12301 gcgctgggca ccaatctgaa atcgcgcgct gccgccaaag cgcatgtcgt caaagggggc
    12361 tgcctcgtga tatttccggc gggcggggcc tcgaccgtgc ccaagccctg gagcaaacgc
    12421 gccgtcgatc cggaatggaa gaatttttcc gcgcgcctca ttgtttcggc gaaggcgccg
    12481 gtcataccgg tctatttcgc gggccaaaac agccggctgt tccaggtcgc aagccacatc
    12541 agcatgacgc tgaggctctc gctcttcttc aaagaagtgt acgacaagat cggcagcgag
    12601 atacatgttc gcatcggcgg gcttattccg tacaaggagc tggaacattt ggaccgcaag
    12661 gatctgatgg aacatttgcg caaggcgacc tatgcgctgg gtgcagatct gccggatgct
    12721 gagattcgcg gcaaacgtcc gcgccctttt cccccgcccg gcaaacgctc atgaacgcgc
    12781 caaacaataa gggcgcgaat ccgcacgtcg gccttctggt gacatgcctt gccgacatgt
    12841 tccgcccgga ggtcggcttt gccgcagtga agctactcga acaggcgggc tgcgatgtcg
    12901 aggttccggc gcaaagctgc tgcggccagc cagcctataa tgcgggcgat gcgaaggacg
    12961 cggctgcact ggcgaagcag gtgatcgcca atttcgaagc gtttgatttc gtggttgtgc
    13021 catccggttc ctgcggcggc atggcgaagg tccattatcc gcatctatta aaggacgatc
    13081 cagcctgggc cctgcgcgcg aacgcgctgg ccgcgaagac ccatgagctg ttctcgtttc
    13141 tggtgaatgt gcgcggaatg aaaaatgtcg cggcggcatg caacggccgg gcggcctatc
    13201 atgattcctg ctcgtcactg cgcgagatga atgtgtcggg cgaaccgcgt ctactgctgc
    13261 aatccgttga cggcctgagc gtccgcgatc tcgcggaaaa ggaaacctgc tgcggcttcg
    13321 gcggattctt ctcggtcaaa tatcccgaag tctccgcccg catggccgac gacaaaatcg
    13381 ccgatgccaa agccacgggc gcgcagatga tcctggcagg tgatcttggc tgcctgctgc
    13441 atctggcggg acgcctttcg cgcaaaggcg aggccttgca cgtgcgccat gcggcggagg
    13501 tgctggccgg catgaccgat ggtccggcaa tcggtgaagc cgagtgagca cggatgcaag
    13561 ccaatttccg cgtgaggcgc gcgccgcact gcatgacgtg cggctgcagg gtgcgcttgc
    13621 gcatctcaaa gagggattcc agcacaaacg cgcggccgcc tttgccggtt tccctatccc
    13681 gttcgaagca ttgcgcgagc aaggacgcgc catccgcgat tcggcactct cgcaactgga
    13741 cggcctgctt ctgcaattcg aaacacaagt cgaggccgcg ggcggccacg ttcactgggc
    13801 gcgcgatgcg gccgaggcgc gcgatgttgt caccggcatc ctgaagcaag cgggggcaaa
    13861 aaccgtcacc aagggcaaat cgatggtgac tgaagagata gagctgaatg cccatctcgt
    13921 ggcgcaggga atcacacctg tggaaaccga tctcggcgaa tacatcatcc agcttcgcga
    13981 cgagaagccg agccacattg tggcgcccgc gttccatctg cagcgcagcg atgtggagga
    14041 gagctttcgc acggcgcacc agagcctgcc cgaggcgcgc agtctggatg accgtcccgc
    14101 tctcgtcggg gaagcccgcg cggtgctgcg ccggcatttc gaagccgcag atgccgggat
    14161 taccggcgcc aatttcctga ttgcggaaac cggcaccgcg atcatcgtca ccaatgaagg
    14221 caatggcgat ctgacgcggc ttttgccgcg cacccatatc gtggtgacag ggatcgagaa
    14281 gatcgtcccg cgtcttgccg atgccgccat tctgctgcgc ctgctcaccc gctccgcgac
    14341 gggtcaggag atcacgtcct atgtgagctt catgagcgga cccaagcgca aagaggatgc
    14401 agacggtccg gaaaatttcc acgtcgtgct gctggacaac gggcgtagcg ccttgctttc
    14461 cagcccggct cgggaggtgc tgcaatgcat acgctgcggc gcctgcatga atcattgtcc
    14521 cgtctatggc gcggtcggcg gccacgccta tggcgcgacc tatcccggac cgctcggcgc
    14581 agccctcgat cccggactcg atgggctgac caagacctat catctgccca atgcgtcgag
    14641 cttttgcggg cgctgcgaaa ccgtgtgccc ggtgaagatc ccgctcacgc gcatcatgcg
    14701 ccattggcgc aatgaggcgt tcgcgcgcgg tattccgtca tggtctttcg atgccggcct
    14761 caaaatgtgg agctggattg cacggcaccc ccctctctac gcgatgggaa ccgcgattgc
    14821 cgcacggatc atgcgcgcca tcgcgggtaa gcatccgcgg ctgcgttcgc tccctatcct
    14881 atcgggctgg ttcgccgcgc gcgatctcgc caagcccgcc gccgtgagtt tccaggcacg
    14941 atggcacagc cgccgtcctg ggaaacgcca tccatgagcg cggcgcgtga gactattctg
    15001 caggccatcg ccaaagggcg cggcgtggcg aagcctgtac cggattacat actcccttca
    15061 tggcccggag agaccggcgc tcatttcatc accaaagcca aggcaagcat cgccgccgta
    15121 catgaaatcg tgtcgcccga ggatgccccc gaaacaattc tgtcgatcct gatcgccgac
    15181 ggccttacgt cacgcctgca tcttccggca gcatccccgc tgaacgcgct cccctggcat
    15241 cgcgcaccgg gcctcacgct cctccctgag ccgcccgacg gcaatgacag cgcgttttcg
    15301 atggccgatt acggcattgc cgaaaccgga acgctggtct tcttttcggg accgcgaagt
    15361 gcttcatcct ggcattttcg ccccgggtgc gagttcgtgc ttctgtcccg caatgccatt
    15421 ctgccgcgcg tcgaggatat catcgcgctt atcgccccct ggaaatcgat gccggcgacg
    15481 ctcaatctcg tcaccggacc ctcgcgcacc gccgacattg agcagaccat agaattgggc
    15541 gcgcatggac cacgggcgct gcacattctg attgccggct aaacggttgc agaccggcaa
    15601 gcgcctctat cctaacagga tttatgcttc gcaaaccggc gaaaccaagc attaattccc
    15661 ctgctctact attgcgatcc gcatcgcgaa attcgcgtgc acagaccggg ggacatatgc
    15721 gcgcccaaca atccttatgg accgcacatc agggctggac cggacattcc ggcggcccgg
    15781 ccgacctctt gcttgctttt ggcggcacgc gggccgttac cgaccgcgac ctctggaacg
    15841 aactcgcatc gcgtcacccc ggcgcgatcc tgctcggctg cagcacgggc ggcgagatcc
    15901 atggctcgga tgttcttgac gaaagtcttt cggtgacatc tctgtctttc gcgcgcaccc
    15961 ctttgcggtc cgccgaagcc attgtcggca gcgcagccga ttccttcaag gcaggccagg
    16021 aaatcggcaa gagcctgtat gcgctggacc tcaagacaat cttcgtcctt tccgatggca
    16081 cgcgggtgaa cggcagtgaa ctcgtgcgcg gcatccggac ctgcacggga aacaaagtta
    16141 tcatcaccgg gggccttgct ggggatggac cgcgcttcgg caccacctat gtcggcctca
    16201 acggcacgcc cataccgggc cgggtcgcag ctgtgggatt ctacggcgaa gccatcgcgg
    16261 tcggtcatgg cagcgccgga ggctgggacg tattcgggcc agagcggaac ataacgcgct
    16321 ccaaaggcaa tgtgctttac gagcttgacg gaaaacctgc actggatctc tacaagcatt
    16381 atcttggcga ggacgccgaa agactgccgg gaagcgcact gctgtttccg ctgaaggtct
    16441 acccgcgcga ttgtgccggt gaggccttga cccgtacgat tgttgcggtg agcgaaaccg
    16501 atcgcaccat gacctttgcc ggcgacatgc cagagggctt cagcgcccaa ttgatgcgcg
    16561 gcaatttcga ccggctggtc gaaggcgcag ccgatgcggc ccagcaagcc gcactttcgc
    16621 aactcggaga tcaggttgcg attctcgtga gttgcattgg gcgcaaattg cttctgggtc
    16681 agcgcatttg cgaagaggtc gaagcggtcc aagatgtctt cggacaaaaa acggcgctga
    16741 cgggtttcta ttcctacggc gaggtttctc cgcatgccac gacgcattgc gcggaactgc
    16801 acaaccaaac catgaccatt accgtcttcg gtgagagcac tggtgcataa gcttctcgcc
    16861 cggcaattgc gtgcggccac taaggaggga gccggcatcg atctcgacgc attgatcgcg
    16921 atcgtggatc aaacctatgc cgaattcgac cgcgaacgcc ggctgaacga tcgtgccgcc
    16981 accctcatgg aggatgagct gaaggccgcg aatgccgagg ccaagcgcga acacgacatc
    17041 gtactggcgg cgattctcga caatgcgtcg gacggaatgc tggtcctgcg ggggaacggg
    17101 gaaatcgtca tcgccaatgc cgcggcggag aaacagttcg ccgcatctgt gggagcgctc
    17161 accggcatgc ggatcgccat gcttctgggc gaggacgccc atgcgatcgc gcgcggagaa
    17221 tatgggcagg gcgcaatgcc ggaactctcg ggcatgacgc tggacggacg cgcctttccc
    17281 atcgagtatt cctgcgccga tctggacgtg tccggcgaca ggcgtcagct ctggatgatt
    17341 cgcgacattt cggagcgcgt gcgcgctcag cgcgaaatca tggaaagccg catgcgcttc
    17401 caggatttcg cggaagcctc ctccgactgt ttctgggaga tggatgcgaa cctgaaacaa
    17461 gtcgaggtca gctccgcctt acagtccgaa cccgtcgcga atttgtcctc gctcctggtg
    17521 cccggtccgg acgggctcac gcccaagggg gttgtggaag agggctggcg cgcattccgg
    17581 cagaatcttt cggcgcgcca gcgttttcgc ctacggctcg atatcaccgg aagcgacggc
    17641 gaaatattat atgtggccgt cggaggcaaa ccggttttcg atctcgatgg cggcttccgc
    17701 ggctatcgcg ggacggcgcg cgatgtgacg cgcgaagtcg cggcgcgcga agcggcgcgg
    17761 cgggctgagc gccgcctgat cgaagcgatg gatgccgcgc cgtgcgccgt agcgctgctg
    17821 gattcgcgtc tgagactcgt gagcggcaat tccgcactga ggaccatggc atcggcaaaa
    17881 ggcggccgtc tgccgttcgg cggatcgttc caggactttc tcggcggcct gaaagccgac
    17941 gatattgccc cacaaagttt gcgcggcctc gtccaaaccg gcgagatgcg ggaaatggcg
    18001 atcggtggtt cgtggtatct ggccgcggcg ggcagcttgt cggatggcgg gatggttctc
    18061 accttttcag acgtcaccgc gctcaaggaa cgcgagcgcg agcttgccga gtcgaagcta
    18121 tcggccgagt ccgccagccg cctcaaatcc cagttccttg ccacgatgag ccatgagctg
    18181 cggacgcccc tcaacgcgat tctcggtttc tccgaagtca tccgtgacgg cgtattcggg
    18241 cgcagccaga acgcctgggg aaaatacgcg gaatatgcct cctcgatcca tgccagcgga
    18301 cgccacttgt tgtcgctgat ctcggagata ctcgacctct ccaagatcga ggcgggaagt
    18361 tatgtcctcg atctgcgcac gatcgatttg catgaggtgg tggaggacgc cctcaccatc
    18421 gtgtctccgg cggcgatgaa agccggtgtc cagctctgtc cgatcctgcc ggccggcgag
    18481 atctggctca ccgcggacga gcgcgccgtg cgccagatcg ctctcaatct tctttccaac
    18541 gccgtcaagt tcactcccgg tggcggcagc gtcacaatcg aggtgagcac ggctggcgaa
    18601 gaagcaaaat tcaccgtcac cgacaccggc atcggtatcg acaaggcaca cgtatcgacg
    18661 gtgttcgaac tcttccgcca ggtggattcc agtctccaaa ggcgccatga gggcacaggg
    18721 ctgggtttgg ccatcacgaa aaggctgatc gaactgcatg gcggcacgat ttcactcgaa
    18781 agcgaactcg cggtcggcac caaggtgagc gtgacgtttc cggacgcgct tgccagaacc
    18841 agctccgcgc ctattctcaa gggcgccgcg gcgtagcgcg aattcgcgcc ttcgcggtct
    18901 tgcgtgcgtc aagcccggcg gtctactagc atccgtgccg ggtcccttga gatgccgctt
    18961 cctccatgtc cgagtcaccg ccagacgtgc cgagcctgcc gccttggctg atgcgactgc
    19021 cgcgcgtgtt ccggcaccgc aattaccggc tgttctatgc cggccagggc gtggccctga
    19081 tgggcgcgtg ggtccaaacc gtggcgctct cctggctcgt ctaccgcctt agtcattcgg
    19141 tgtttctttt ggggcttgtc tccttcgtcg cgcaggcgcc gatcttcttc atcacgccct
    19201 ttgccggcat gatcgccgac cggcacgacc ggcgcttcgt cttcatgatc acgcgtgctc
    19261 tttgcatgat tcaggctgcg atcctcaccg tcctcacact caccgacaat gccggcatcg
    19321 gaaccatcat cgcactggcg ctggtcctgg ggatcatcac ggcggtggaa acgccggtga
    19381 gacagtcctt cacggtggaa atggtcgggc gcgaggatct gcgccaggcg attgccttca
    19441 acgccatgat gttcaatctc gcgcgcacct tgggaccggc gatcggcggg gtgatcgtag
    19501 cggttcttgg cgaagggctg tgcttcctgt tgaacacgct gtcctatggt gcggttctga
    19561 caagtctgtt cctgatgcgc acaaaatcgc gcactggccg gccaaccacc cacccctggg
    19621 acgatctgaa acagggattc caatatgtca cgcgtcaccc gcatctgcgc atggtgctgt
    19681 ttctttccgc cacatgctcc tgtttcggaa cctcctatct cgcgctgatg ccggccttca
    19741 cgcaggacat cctgcatcaa ggcgctgagg cgctcggtta tctcatcagc gccttcggcg
    19801 tgggcgcgat ttcgggggct atcctggtgt cgcggctgca cgagcggcac ctggcattga
    19861 cgcctaccat catggcggtg cttctgggcg cgagtctgat aattttcgcc aacacccaat
    19921 cgatgctgct ggcgctcttg ttcgttgtgc ccactggctt gggctatctc ggcatcgcgg
    19981 tctccagcaa cacgcaagta cagacgctgt ccgacgatgg gatgctgggc cgcatgatgg
    20041 ctttctacgc gatgggagca ctgggaactc ccccgttcgg agcgctgctg ctcggctata
    20101 tttccgacct cgtgggcgtg cgcagtgcgt tcatgttgag cggcgccatc tgtctcattg
    20161 ccgcagggct ctcgctgatg agtctcagac gccgcggtct cgtggatttc gccgtccttc
    20221 gcgaggcgca ggaaccatga gcgtcaatgc ggacttcgac ggcctgaagc ggatacgcga
    20281 agcgatcgcg ctgcaccagc aaggccggct tgcggaagcc gagcggctct accgcgatat
    20341 tctcgcacat acgcccgatc aacccgatgc gcttcacttc ctgggcatat tagaaggtca
    20401 gcggggccgg catcagccgg ctctgcaatt gttggatcgc gcactggcgg tcaatccgcg
    20461 tcatcccgca attgcctata atcgggcaaa tcttctgcgt gacatgggcc ggctcgaaga
    20521 cgcgctgacc ggctacgata cagccctcgc catcaaaccg gacaatgtcc tcgcgctcgg
    20581 caatcgcggc gcggtgctgc atggcttagg acgctatgac gacgcaatgg cgagctatga
    20641 acgcgcgctg gcgctcaagc ccggcgattg cgacacgcat tccaatcgcg caaacgccct
    20701 ggccgccctt ggacggcatg aagatgcgct cgccggctac gacaaggcac tggcggggaa
    20761 gcccggccac gtcgccgccc tttacggccg gggcaatgcg ttggcgaaac tgggaaggcc
    20821 cgaggaggcg cttgcttcct atgatcgggc tgtccagtgc gatcctggca atgcagagct
    20881 tctcgccaat cgcgccatcg ttctgagcga tctcaaccgc tacgaggaag cgcttgcgga
    20941 attcgcgtca tcgattgcac gcgcaccgga gaatgccgaa tcgttcaaca gtcgcggcct
    21001 cgtattcatg cagcttcggc gccatcggga tgctctcgcg gatttcacgc gagcctgcga
    21061 actaaatccg gctttcgcta atgctcttta tgggcgcgct tcggcccttg tcgaattgaa
    21121 ccgtcacgac gaggccattc cggccttcga acaattgttg cggcaagcgc cggactatcc
    21181 ctatgggctt ggcatgctgg tctatgccca gaggacagct tgcgattggc gcgacctatc
    21241 cgcgagcgct gcattggccg atgccatcgc ggcaggaaag cgcgtcgcta caccgcttgt
    21301 gcttctcgcg atctccgatt cgccttccct gaagctgcgt tgcgcggaag ttctgatcgc
    21361 ggacaaattc cggcccgtgg aggagcctct gtggcgtggc gatcgatctc gtcatgagcg
    21421 gattcgcgtc gcctatcttt ccgcggattt ccgaccccac cccgtggcca tcctgatggc
    21481 cggcgtattc gagcatcacg accgcgagcg tttcgagacc atcgcgattt cctatggtac
    21541 ggatgataaa agcatgatgc gcgcgcgact gatgcaaagt ttcgagcgct tcatcgatgt
    21601 gcgcggcaag agcgacaggc aggtagccgc cctgatccac gatatggagg ccgatatcct
    21661 cgtcgatctc accggcctca ccgccagcgc acgccccgga atcctcgcgt ttcgccccgc
    21721 gcccgtgcag gtgaactatc tcggcttcgc tggctctctc ggcgcccctt acacggacta
    21781 tatccttgcc gacacgatcg tcatcccgga agatcagcag gtgttttacg cagagaaaat
    21841 ggcttatctg ccggcctcct acctgcccca tgattccaag cgccgcatcg ccgaacgcgc
    21901 gcccgcccgc ggcgaactcg gtctgccgga ttcgggcttc gtctttgcgt ctttcaacaa
    21961 cagctacaaa ttcacccccg aaatattcga cgtctggatg cggctgttgc gcaaaatcga
    22021 gggtagcgtg ctgtggttgt ccgcatcgaa cgcggccgcc cagcgcaatc tgaagcgcga
    22081 agccgaagcg cgcggcgtcg ctggacatcg aatcgtgttt gccggattcg tgccccaagg
    22141 tgaagatcat ctcgcgcggc tggccgccgc ggatcttttt ctggacacgt ttccctataa
    22201 tgcccacacg accgcgagcg atgcattgtg ggcggggctt ccgctcttga cctgcaaagg
    22261 ccagagtttc gccagcgcgg tggcggccag cctgctgcag gcctgcggct tgccggagtt
    22321 gatcaccgat tcactggcag cctacgaaaa ccgtgccctc gatctcgccc gcaacgctgc
    22381 cgcgctggcc gatataagag cgaaactcag cagcaatcgc gatgtttgcg ccctcttcga
    22441 cacggcccgt ttcacacgaa atctcgaacg cgcttacacg acgatgtgga aacgttcgca
    22501 atccggcacc gaacccacca gctttcacgt cgaaccggtg gcagacccat gagcgaggcg
    22561 gcgctcaaac aggcgtggag cttgcagcaa gcggggcaac atggcgaagc cgcacggctc
    22621 tacgccgatg ttctgcgcac aaacccgcgc aatttcgatg cgcttttcca attgggtaac
    22681 atctatctcg tcaccggaca tcccgccgac tctgaacggc tttatgcccg cgctgcacag
    22741 atcaattcgc aatcacccga gctgttctat aatcgcgggt gcgcggccat gagcctttcc
    22801 cgccacgaag atgcgcttgc cgcttttgcg catgctcttg cgctccgtcc cgatttcacc
    22861 gaggcacgca acaatcgtgg cgtcaccctc ctttcgctca agcggcataa ggaggcacta
    22921 gcgtgtttcg accgcgtact ggcggacagg cccggccttg ccactgttca gaacaatcgc
    22981 gccaccgctc ttctgggttt gcttcgccag gaagaagccc ttgccgctgc cgaactcgcc
    23041 ttgtgcgaga atccacagga tgcgcatgcg ctttacaatc gtggcgcggc gctgatgatg
    23101 atcgggcggc ttccggaatc cctgtcgcag ttcgacaagg cgctctccgt caatcgcgat
    23161 tatgcggacg ccctcatcta tcggggtatc gtgcttgccg tgctgaaccg tcatgaggaa
    23221 gcgctggcca gctataacat ggcactcgta atccggccag gcgacatcga gattctttat
    23281 aatcgcgcga cctcgctttg ggcgctgaaa cgcttcgagg aagcaattcc cgattgcgaa
    23341 gaggtcttaa agcgcaatcc gcatttcaaa tatgcgcgcg gcaatttggt gcagagcagg
    23401 ctgcagtgct gcgactggca gaatttgacg gaggataagg taaaaatcgt gacggatttg
    23461 cacaatggcg tgctgaccct caatccgctg cagagcatcg tgctgttcga ttcgccggac
    23521 atgctgctgc aagccgcgcg gctctggatc gccaacgaat gcgcgcccgc gatgacgccg
    23581 ttgtggcagg gcgaaatcca taagcacgac cgcatccgta tcgcctacat gtcggctgat
    23641 tttcgtatgc atgccgtggc gctgctggtg gcgggcgttt tcgagcatca cgatcgaacg
    23701 cgtttcgaga ccaccgcgat ttcgataggt cccaacgatt caagccccat gcgcgcgcga
    23761 ctggaaacgg ccttcgaccg cttcatcgac gtgcaggacc gcaccgatgc cgaggtcgcc
    23821 gaactgatcc gtgagatgga gatcgatatt ctcatcgacc tcaccgggtt tacgcaacac
    23881 ggccgcaccg gcattctggc gcggcgtccg gcggggattc aggtgaactt tcttggcttc
    23941 ccgggcacca tgggcgcgcc ttatatcgac tacatccttg tcgatgaaac tgtcattgta
    24001 gaagccgatc agcgctattt cgaagagaaa gtggcgtatc tgcccgaaac ctatctcccg
    24061 aacgatttca cacgcgccat cgcagaacac tcgccgagcc gcgcagaatc gggactgcct
    24121 gagaatggtt tcgtgttctg caccttcaac aatctttcca agatcacgcc tgagacgttt
    24181 gatgtctgga tgcgcctgct cgaaaatgtc ccggacagcg tgttgtggct ctcccaggcc
    24241 aatccttcgg ccatccgcaa tctgaggcgc gaggcgcaag cgcgcggcat cgcgggcaac
    24301 cggattctgt ttgcgccgtt cgtccaagca cccgaagaac atctggcgcg gctgcgtctt
    24361 gccgatttgt tcctcgacac gcggccctat aacgcccacg caacggcatg cgacgcgcta
    24421 tgggcgggtg tccccatggt gacgcagata gggtccagct tcgccggacg cgtgggcgcc
    24481 agtcttttga ccgcgatcgg ccttcccgaa ttgatcgcgc attccgaaga ggaatatgag
    24541 gctctcgccc tgcggcttgc ccgcgatccc acggcactcg cgctcctgaa ggagaagctt
    24601 gcgaacaatc gcatgacgca tccgctgttc gataccgccc gcttcacgcg cgatctcgaa
    24661 agcgcctatc ttgccatgtg ggaacgtcat cggcggggcg aatcgccccg tggcttcagg
    24721 tccagcgaaa ccattgcaca atgagcggtg gcacgatcca aaaagcgttc ggtctgcatc
    24781 gtgcgggcaa actcgacgaa gccgcgaagg tttacagcga aatcctgcgc agcgaacccc
    24841 gtcaagtgga tgcactctat ctgcttggat cggttcattt tcaacgtggt gaatttagcg
    24901 atgcgctggc ccgtttcgat gaagcgcttg ccattaagcc gcgcttcatc gaagccatgg
    24961 ccggacgcgg cgcggcgctc tcgaatttcg gccggcagga agaagcgttg acggcctatg
    25021 agactgtgct ggcggccagt cccgatcacg cgcagagttg gaacaatcgc ggcaatacgc
    25081 tgcttgttct gggtcgcgag caagacgcgc tcgccagcta cgaccgggcg ctcagcctgg
    25141 tgccgaatta cgtcgatgcg tggcgcaatc gcgcaaccgc cttgctgcgg acaggacgga
    25201 tcgaggaggc gctggcgaac ttcgagaggg cgcttgaact caagcccgat ttcgccgttg
    25261 cgtgggagga ctacgccacc gtgctggcaa gacttggccg gcgcgaagat gccatcgtgg
    25321 cctatgatcg cgcgctcgcc ctcacgccgg acaatccgga cctgctgtat aatcgcggca
    25381 acctccacgc catcctgaga cgttacgacg aggccatcca tgactgcgag gccacgttgg
    25441 cgcgcgcgcc cgattatccc tacgcccgcg gcgtgctgat tcattcaaag ctgcaaagct
    25501 gcaactggcg gggattggat gcggagattt ggaatatctc gatggcgctg gccgcgggca
    25561 agcgggtggt atcgccgttc aatctcaagg cgctgtccga ttcgccgccg gaacatttgc
    25621 gcgtggcaca gatctggacc gccaacgaag cacctgcgcc gccgtttctg gcgtccaaac
    25681 ctctcgggca aagcccgcgc gacacgcatg accgcatccg cctggcctat gtctcaggcg
    25741 atttcaacag cagcgccgtg gcgaccctga tggcgggcgt gtttgaacat cacgaccgca
    25801 gaatgttcga gacgattgcg gtctccttcg ggcccgccgc cagcacgccc acgcgcgtgc
    25861 gattggaatc cgcgttcgaa cgcttcgtcg atatgcgggg caggagtgac ggcgaaatcg
    25921 ccaatctgtt acgcgacatg gaagccgata tcgccgtcga tctgatggga tataccggcg
    25981 aatgtcgcac cgccatcttc gcccaacgcc ccgctcccgt gcaggtgaac tatctcggct
    26041 ttcccggcac catgggcgca tcctatatgg actatatcat cgccgattcc accgtcgtgc
    26101 ccgaagatca gcagcgtcac tacgccgagg ccgtcgccta tctgccccat tgttatttgc
    26161 cggccgaccg cgcgcgtggg gtggcagaga ccattcccag ccgcgccgag gcgggattgc
    26221 cgcaagacgg cttcgtcttc gtctcgttca acaacagcta caaattcaac cccgtgatgt
    26281 tcggcgtctg gatgcggctg ttgcgtgcgg tggaggggag cgtgctgtgg ctgccggaaa
    26341 ccaatctggc agcgcggcgc aatctcgtgc gtgaagccga agcgcgcgcc gtggcgcccg
    26401 cgaggatcgt gttcgccccc cttgtgcccg atgccggcga acatctggcg cgactctcgc
    26461 ttggcgatct gtttctcgat acgctgccct acaatgcgca caccactgca agcgatgcgc
    26521 tctgggcggg tgttccggtt ctgacgcaga tcggcaacag cttcgcgggg cgcgtggccg
    26581 cgagcgccct gaaagccgtc ggcctcgccg aattgatcgc ccgcacaccg gacgaatacg
    26641 aggccatagc gctggcactt gcgcgcgatc ccgtgaggct tggcgaaatt cgggcaaagc
    26701 tcggccgcaa ccgcctgacc acacccttat tcgatacggc ggggttcact cgcgatctcg
    26761 agaccgtgtt caccaccatg tgggcgcggc accggcgcgg cgagatgccc gcaagctttg
    26821 ctgtcgcagc cacggattaa ttatgcttcg atcccgcccg tcatgaacga tcacattctg
    26881 cagaacgcgt ggcggctgca tcaggcagga caattcgccg aggccgcacg gctctacaat
    26941 caggtgctga gcgccaatcc gcgccatctg ggcgcgctgc agatgctcgg ctatctccat
    27001 ttccagcgcg gtgagctgaa cgatgccgat cgcatcatgg cgcgtgcgct gaagctcgat
    27061 ccgacatcgg tggatgcgct ctacaatcgc ggctgcgttc tgcaggcgct cggcaaggaa
    27121 cgcgatgcgc tggccagttt cgaccgggcg ctcgcgctga agcccgatta cgcgcctgcg
    27181 atggccaatc gcggcaatgt gctggcgcgg ttggggcgct ttgccgatgc gctcgcgagc
    27241 tacaatctgg cgctggcctt gatgccagca tccgcagaat tgctgctcaa tcgcggcaat
    27301 gcgctattcg aactcggccg ccacgccgaa gcgctgacga gctacgatca ggcgttggcg
    27361 aaggaaccgc aagttccgat cgtctggcac aatcgtggca atgcgcaggc cgaactcggg
    27421 cgtcatgtgg aggcgttggc gagctttgac cgcgccattc aattggatgc ccgctatggc
    27481 gacgcctatt tcgggcgcgg cgccgcactc gtaaagctcg aacgcgacag cgaggcgctc
    27541 gcagatttcg aaaagacatt ggcgctcgat ccggcccatc tcgcagcgct ccacaatcgg
    27601 ggacgtatat tgcgaaagac cctgcggatc gccgaggcgc tcgccgcgtt cgaggccatc
    27661 ctcgcgttcg agcctaccta taccgaagcc atgatcgaca agggcatcac gctgatcgcc
    27721 ctgaaacgcc acgccgaagc gctcgccgcg ttcgatagcg ccctaacgct cgcgcccggc
    27781 aatgtggaag ccctgacgaa tcgcgcgagc gcgttgatcc gcctgaagcg ttacgaagaa
    27841 gcccgcgcgg attccgtccg cgcgacagcc gcggaacctg cgaacgctgc cgcctggcac
    27901 aatctcggcg gcgcactctc cgggctgaag cggcctaagg acgcgctcgt ctgtttggac
    27961 aaggcgctcg ctctcgcgcc ggacaatggg gcaagctgga acaatcgcgg cgccgcgatg
    28021 gtggtgctga aacaggagga ggcggccctc ccttatttcg acaaggcgct ccaactcgat
    28081 ccgtccgatg tcgacagctg ggcaaaccgc gcgcgcgccc attctaatct gcggcatttc
    28141 acggaagcca tcgccgacag cgaccgggcg ctcgccatcg accccgatct cgcggcggca
    28201 aaacgcatcg gcatccacgc gcgccttcac gcctgcgctt gggagcggcg tgacgacgac
    28261 aaacggcgga tcacggcagg cctcacagcg ggagaacgga tcatcaccgc gctcgaccat
    28321 cgcggtctat gcgattccga aagcgagaac ctcatcgtcg cgcggttatg ggccatggaa
    28381 gaatatcccc ccgctcccaa tccgctctgg cgcggcgaac gctattccca cgacaaaatt
    28441 cgcatcgcct atgtttcgac cgatttccgg gcccacgcgg ttgccttcct catcgtgggc
    28501 tcattcgaac atcacgacaa gcagcgtttc gaaaccaccg ccatctcgat tcatccaggc
    28561 gacggcagcg agacgcgaaa ccgcatcgaa accgcgttcg accgcttcat cgatgcgacc
    28621 gcgatgagcg atgccgaagt cgcagcgcgg ctcaaggaga tggaaatcga tatcgccatc
    28681 gatttgaacg gctatacggg cgacgcgcgc accggcattt tcgcgcatcg tgccgcgccc
    28741 gtgcaggtga attatcttgg ctatcccggc accatggccg caccctatat ggactatatc
    28801 attgccgatc cgatcacgat cccggccgag caccggacgt attacagcga gaaagtcgtg
    28861 tatctgccga acgcctatca ggccaacgac cgcaagcggc gcatcgcgga gcgcacgccc
    28921 acacgcgccg aatgcggatt gccgcaagag gggttcgtgt ttgcctgctt caacaacacg
    28981 cacaagatcg gacctgagat attcggcatc tggatgcgcc tattgcgcga ttatgccggc
    29041 agcgtgttgt ggctgttcga ggacaatgcc gtcgctgccc aaaatctccg acgtgaggcg
    29101 gccgcacgcg gcgtggcccc ggagcggctg gtgttcgcgc cccgcatgat gccgcccgag
    29161 catctggccc gcacgcggct cgccgatctt ttcctcgaca ctcttcccta caatgcccac
    29221 accacggcga gcgacgcgct gtggatgggg ttgccggtcg tcacttgccc cgggaccacc
    29281 ttcccctccc gcgtggcggc aagtctgctc catgccatcg ggatgccgga attggtgaca
    29341 tcctcgctcg cggaatatga ggaactggtg cgcgttctgg cgaagaaccc tgaacggctt
    29401 gcggaaatcc gggcgaagct cgcgcgaaac cgcgacatcg agcccatgtt cgatacggcg
    29461 gggttcactc gcgatcttga agcggcttac accggcatgt gggaacggca gcaggcgggg
    29521 cgcgcagcgg agagcttcgc cgtgcctgcc gaagcgccgt agatgggcga cggcccgccc
    29581 ccgcccgaac atttggcgaa gctgcatttc caggagggcc gcttcgaatt ggccttgcgg
    29641 catctcgatc tggcgattgc cgccgcaccc gggaccgccc aattgtggaa cagccggggt
    29701 gcggtgctcg cgacgatgac gcgattcgaa gacgctcatc aaagcttcag ccgcgcgttg
    29761 atgctcgcgc cggacttcgc cagcgcgagc atcaatcgtg cccatgtgct gatggcgttg
    29821 caccgttacg aagcggcaat cgccgattac gagcatgtcc tgcgcatcaa tgaccgaatt
    29881 ccgttcgcgc gcggcaatct gatccgcgcc aaactgcaat gctgcgattg gcgcggtctt
    29941 gcggaagaat gggaacgtgc gctggacgaa atgcgcgccg gccggcccat catttccccg
    30001 atggtcgcaa ccactctttg cgatgcaccg caggatcagc ttcgtgtctc gcggattttg
    30061 gcggaaaccc gatatccgcc cgcgcgcaca ccactctgga acggcgaaat ctatcgccac
    30121 ccgcgaatcc ggatcgccta tctgtcgtcg gacttccacg cccatgcgac cgcgacgctg
    30181 atggctggat tgttcgaggc gcatgacaaa acccggttcg aaaccttcgc cgtgtctttc
    30241 ggacccgagg acgccagtcc catgcgccag cgcctggtgc atgcgttcga gcatttcctg
    30301 actgtcgggg acaaaaacga cacggaaatc gcgaagctgc tgcgcgagca cgagatcgac
    30361 atcgccatcg atctgaaagg tttcaccgat caatcgcgcc ccggcatcct cactgcgcgg
    30421 cctgcgccgc tgcaggtgaa ctatctcggc tttcccgcaa ccatgggcgc accctatatg
    30481 gattacatcg tcgcggaccg cgtggtggtc ccgcccgcca atgccatcca ctacaccgag
    30541 aagatcgttt atctgccgca ttcctatcaa cccaatgaca ggacgcgcgc ggccgggaca
    30601 ccgcccaccc gcgccagcga aaatctgccg gaaatcggtt tcgtcttctg ctgcttcaat
    30661 gcgagctaca aaatccagcc tgtgatattc gacatctgga tgcggctcat ccgtgacgtg
    30721 ccatccagcg tgctgtggct tctcgacgac aatccgcaag cccagcgcaa tttgaaacgc
    30781 gaagcggctg cgcgcggcgt agacgcggac cgtctcgtct tcgcgccgcg aaaaccggtc
    30841 gccgaacatc tggcgcggca cagcctcgcc gatatcttcc tcgacacgct gccctacaac
    30901 gcgcacacca ccgccagcga cgcgctgtgg atggggctac cgctggtcac atgccccggc
    30961 acgacatttc ccgcgcgcgt gagcgcgagc ctgctttcgg cgatggggct gcccgaactg
    31021 atgacatcct ctttggcgga atatgaagaa ttggcgaggg cgctggcgcg cgatccgcag
    31081 cggcttgcgg ccatcagggc gaagctcgcg cggtcctgcg aaacttcgcc tctgttcgat
    31141 gccgcgcaat acgcgcgtaa tcttgaatcc gcctatatga cgatgtggga acgccagcag
    31201 cgcggcgatg cgcccgcaac cttcacggtg gacactgccg gctgatcccc acgattattg
    31261 cgggggtggg ccctgggtga tgcggaccag atcgtccggg cgcatccatt tcttgaacgc
    31321 ggcctggatt tcggcgggcc ccagcgcgat atagcgccgt gcggcctctg tcggctcgtc
    31381 gagcggcagg tccaaatccc agcggccggc caacccgcgc gcgatgccgc cgatgctgga
    31441 ttcgcccaac ggtatctgcc ggagcatcag cgccttcaca cgcttgagtt cgtcggtgct
    31501 caccggctcg tcctgcatct tcttcagttc ctgcacgatg atctgatgag ccttggagac
    31561 gttttgcggg tccgacgcgt attgcacgaa ataattgccg cgcgtgcgcc cggcgttcaa
    31621 atccgagccc acggaataga ccagccctgc gttctttctg agatcgatcg aaagccgcgt
    31681 cgaatagaag ctgccgccca gcacggcgct gcccagttcc agcgcgtaat agtcgggatg
    31741 cgagcgcgtg atcgccatgg tctgcgccag gatgacgctg ttctgcaccc ggctttcatc
    31801 cggcacggcg acgagttttg ccttattggg cggcgccacc ggcagatcga ttgcgggctt
    31861 ggggccctcc gcagtccagg cgccgaaata tttctgaacc gctgcgcgcg cagcttccgg
    31921 cgtgatgcgc ccgaccacga cgatggtggt gagatcgggc cgcacggact tcttgtaata
    31981 ggccttgatg tcgtcgagcg tgagcgcgcc gacagtttcc ggcgtcgcat cgcgcaagga
    32041 cggatcgttc gccgggaata gcgcttcgcg cagcgagcgc tgcatcagat aaccgggact
    32101 cttttgctgt gctgcgacca cgcgtgcgac ctgcctcttg atcacatcca tggcggcttg
    32161 cggcaaggcc gggtgcagct cgttgtcggc gagcaatgcg acgccgcggt cgagatcgcg
    32221 tgtcaggacc tgcacggtga aatcggtgcc cgcgctttcc tgcgcgccga tctcgtccag
    32281 cgcctcctgg aaggcaatgc ggtcgagcga ttggctgcca taatcgaaca gctcgccgag
    32341 cacttgggac aggccttcct tgccttgcgg cacctcaacc tcgggccgcg tcttgatgtg
    32401 gccgtatacg gtgaccgtgt cgctgacgtc ttcgggctgt acgatcagag tgagcccgtt
    32461 gggaagcgtg ctgacggtgg gatgcacacc cgatgcgggc accgacaaac ggttgagcgc
    32521 cgcttcggcc cagtccggca atgacgttgc ctgcgcttcc ccgagcgcga tgctctcctg
    32581 gccgccaaaa ccaccagacg ccaccggacg cccgctgccg cgcggcacca tgactgcggt
    32641 gatcgcatgg ttgaggtcga gatatttgcg cgccacgcgg ttcacatcgg cgacagtgac
    32701 tttcttgatg cgctccagat cttcgtccgg agagcgcaag ccgtagaccg ctacggcttc
    32761 ggcccaaatc gaggcaagcc cgtcgatgga attcttttgg aattccgcct gacgttcttc
    32821 ctgaatcttc gctgcatcca ccaattccgc cggcacccca tcgcgcagga ttttctgcag
    32881 cgtcgtacgc agttcgtttt gcgcggcctt cgcgtcggca tccgcggggt atgagatgga
    32941 ggcgtaggcc atgccgactt tgggcagcgg atcgagcgag aagcccgccg ccagcgcctt
    33001 accggcggga accagcgcga agagatcgcc gcgctcgctg ttcagcacat ctgagagcac
    33061 ctccagggcc ggaaaatcgg cgctgtcgag acccggcatg cgcatcgcga gaacggcggc
    33121 gccattggca ttgtcggtcg ggatggagat cgggtcgacc gcgacaagcg ccttgggatt
    33181 gatcgccgga tggctcccca gcgtcttctt cgggatggac tcgaacaggg tgcggatctt
    33241 cgccagcgtc gcttgggggt cgagattccc gaccaccact aggatcgcat tgttcggcgc
    33301 gtaccatttt gcgtagaagg ccttcagatc ggccgccgtc gttttctcga aggacggccg
    33361 cgtgccgagc gcgtcatgtt cataaggcgt tcccgcaaac atgatctcgc gcagtttctg
    33421 gaacatcaga taattcgggc tggagacgtc ctgcgcgact tcctgctcga ttgcgccgcg
    33481 ctcctggttc caatccttct ggtcggcatc gagcccccgc atgcgcgtcg cttcgatgcg
    33541 cagcgcaacg tcgatgtctt ccgacggcac cgtgaaaaga tactgggtga gattctcccg
    33601 ggtattggcg ttgaaattgc cgcccatgat gcttccgata ttggcgagct ggtcggcggt
    33661 cagtcccggg ctgccgcgga acatcacatg ctcgagcgca tgggccgtgc cgggaaaacc
    33721 gtcgggggcg tcattggagc cgacgagata attcaccgac gtggcgacca cgggcgccag
    33781 cgtgttgcgc accaggataa ttcgaagccc gttggcgagg gtcgcgcgca ggacgcccgg
    33841 ctcatcggcg gttgcggccg cgcgcgcttg tgcagcgggc gcgggtggga tcgagacgaa
    33901 attggcaatt ccgatgacgg caagcgcgag gccacccaga gccgcgcccg cgaaaccgaa
    33961 tttgcgcgca ttccgcacgc ctgaattgcc catcatgaac tcccgaaagg ggtggtttgg
    34021 cgttcgttac gccccgccag cgccaagcaa attatcttag cgcggctttg aacccagcgt
    34081 tacaattctt tatattattc acttccccgt cagcaccctg aagcccgcgc gctccagaac
    34141 agggcgagct ttgccgctct tcagataggc caggaatgtc gcggcgtcgg gcttggcgtc
    34201 cttggtcagc gccaccggat agacaatcgg ctcatgcgtg ttttcgggaa acgtgccggc
    34261 cacgcgcacc ttcggttcga cgcgggcatc cgttgcatag acgataccga gcggcgcctc
    34321 gcctcgcgcg acataggcca gagtggcgcg cacgtcctca ccttgagcga gatggtcctt
    34381 gacgctgtcc cagacgccaa gggtggtcag cgcggccttg ccgtagcgtc cggcggggac
    34441 cgaggctatg tcagccagcg caagccgccc acccatcagc gcatcagcca gcttgaagcc
    34501 gggcgcgatg cgcagcgtgg ttttcgatcc cgcaggtgcg attagaacaa ggctgttggc
    34561 cagaagattg gtccgggtcg tccgcgcgat aaggttcttc cggtccagat aatccatcga
    34621 ctcgctgtcg gcggagatga acatgtcggc gcccgcgctc gcttcgatct gcttggcgag
    34681 cgtcatggag ccagcgaacg agaattcggc ccacttgccg gttgcggctt gataatcctt
    34741 gccgatctgc tgcatgacat ccgtgagcga ggccgccgcc agcacggtaa tccggtcgcg
    34801 tgtctgagcc tccgccgcaa aagacagcag cagaagcaga atcgcggaag cggccgtctt
    34861 cagccatcga accggaaaca tttgtgtacc gttccgtgtg atatttcgtt aaatatcacg
    34921 tcgaggtgga tagcggcaag ctctggacga aagcccgcgc gccttgcaac atcacatcca
    34981 tgactcggct ttccatccgc atcgatttcg aaggcgctga agcattcggc cccggcaaag
    35041 tgcgtcttct ggaattgatc gacgagcagg gctccatccg gggcgcggct gcggcgatga
    35101 gcatgagcta tcgccacgcc tggctgctgc tgcaggccgt cgaggatacg ttcggcgcgc
    35161 cggtgatcag tacagccacc ggcggggcaa agggcggcgg cgccaaattg acggaactcg
    35221 gcaagaccgt ggtcgcgcgc taccgtgcga tcgaagcgca ggcagcacag gccgcggcca
    35281 gcgagctgac ggaactttcc aaagccgccg ctggcgccgt ccccgaagaa cgcaacaaac
    35341 gccgcgagcg tagcccccgg taagttattt cccgaccatc acgtcggatg ccttgatgat
    35401 cgcggtgacc ttgtctccgg cggcgagacc caattcgtcc accgcctcgt tggtgatgga
    35461 ggacgtgata tgcgcgccgt tgcccagatc gatccggaca tgggcggtgg tctggccctt
    35521 atgaacctcg atgatggtgc ccttgagctg attgcgtgcg ctgatcttca tctttggctc
    35581 ctgacggatg ttgcggatcg ggaacgcccg ggcagaaaac ccgtttccct tcgcctacgc
    35641 tcctcgccac gatagcattc ttcggcccac agggaatcgt ccatggcgtt cggcaaatat
    35701 ggtggcgcag cgcgcttttt ctgccgcttt gctgccgcgg gcctacgcag cagtcgcaga
    35761 tattcgctgc gagagcgcct cgcgatgctg ccggtttcgg gcgcgcccgt ttcgcatccg
    35821 gtttcgatcc ggtgggatga gcaccagatt ccgttcatcg aagcccaaac agatgcggat
    35881 ctcgccaccg cgctcggaat cgtgcatgcg catttgcgct tgggtcagat ggaactgatg
    35941 cgcttcatcg ccagggggcg cgtggccgaa gtcaccggcc atatcggcct tcccatcgac
    36001 cggctattgc gcactttcga catcgggcgt gccgtgcccg acatccttgt gggcctgccc
    36061 cacgaaacgc gaaactggct cgaagccttt gtgcgcggca tcaatcattg tgccgcgcac
    36121 gcgcccgcgc ttccgcgtga attcgccctt ctcggtctga aacgagagtt gtgggacctc
    36181 cgcgatatcg tgctgctggg acgcctggtc gccgccgacg tgaattggat cgtatggttg
    36241 aggctgttca aattccgcaa ccttccagga tggccggagg tttggcggca tttgcttcgc
    36301 cacgacacgc tctccttcga gagcgggagc gatgcgggcg atcttggacg cagcttcgcg
    36361 ggcgcggcga tccgctccgg gagcaattcc tacgctgtcg cggccgcgcg gagcgaaagc
    36421 ggcggagcgt tgatcgccaa cgatccgcat ctttcgttca tgctgcccaa tgcattcctg
    36481 ctcgccggat tccagtcacc gtcctaccat gcggtgggca tgatggtgcc gggtattccc
    36541 ttcatcggca tcggccgcaa tccggcaatc gcctggggcg gagcgagcct gcatgcggcc
    36601 agcagcgatc ttgtcgcggt gccaaagggc gcgtccttgc ggagccgcga ggagcgcatc
    36661 gcggttcgcg gcgaaaaaga tacgatcctc cacattcgcg aatcggcgtg gggaccggtg
    36721 atcagcgatt tgcccgcgct gcgtagccag gaaacgctgg cgctgcgctg gatgggccat
    36781 cggtccagcg acgagtttac cgccatgctg cgggtgaacc gcgcacgcaa ttgggaggag
    36841 ttccataccg cattcgacgg ttacgcccta cccggcctcg agatgaactg tacggaggta
    36901 tcgggcgata tcggccagtt gatggcggcg aaactgccac gacggagaaa cgccgaatcc
    36961 gacgacatgg tgagccagcc ggacaatggc tgggacacgc ccatgacaag ctgcgatttg
    37021 ccctgtcgct atcaacctgt ggaagggttt ctcgcatcgg ccaatgcgcg ccccgacaat
    37081 tgcggcgcca ttgtcggctt tcatttctcg ccgccaaatc gcgtacggcg attacgggag
    37141 ctgcttggcg gacacaggat attgtcctta tccgcgctga tgacgattca gtgtgattgc
    37201 catctgtccg aaagcctcac ccagcgcgat caaattctgt cctggcttca gggcgcagga
    37261 cacggcgcac gggagaagca gttccgccgg gcgctgcgga attggaacgg cgattacgac
    37321 gcatgctcgc atggcgctgc cgcttttgag cttctgttcg tccatctcgc acgtgccatg
    37381 gtgtccgtca cccgccgagc ggcctatgat gccgcctggg gaacgcgcgc gctcatctgg
    37441 gccgacatca tggcagcgcc ggccgagacg cgcgccgatg cgcttgggcg tgccgtcgtc
    37501 cacgccgcgc gcgatttttc taaagcctcc gaatggggcg cgcgccatcg cctgcggctc
    37561 gctcacccgt tcggaatgat tccgctcctc ggacgccgct atcggctggc cgatattccc
    37621 gcatcgggca catcggagac gctgatgacg accgcgcacg gcttgaccaa caagcgccat
    37681 cacgcgcgtt acggttcggt tgcgcggcac atctcggacc tgtccgaccc ggacgccaat
    37741 catttcgcgc tgttgggcgg ccaggacggt tggttcggaa gcatcaattt ctccgatcaa
    37801 atgacgctgt ggcgccgcgg taaatatgtg cgcttgccgc tgaccccggc agccgtgcgc
    37861 gaacgcttca cccatctcac ggtgctcgcg tcatgatcga cgcaaccccc ctgctcaggc
    37921 tttatgcgcg cgcgcgcgcc agcgaactcg atcatcagga cgccgaggac gcgcagcgtg
    37981 gacaactctt gagacttctt cgtcatgccg cgaacacgcg cttcggcatc gaccatggat
    38041 tttctgcgat caacggcatt accgattttc aggcaagcgt gaaacttcgc cgctacgaag
    38101 acatgtggcg cgactattgg caaaagccgt ttccccgcct tgtcgattgc agttggccta
    38161 aaaccatccc ttatttcgcg ctcagttccg gcaccaccag cggtacaacg aaatatattc
    38221 cgtatacgga agacatgcat cgcgccaaca gccgcggcgc caaggacatg ctggtgcatc
    38281 atctgcgcaa ccggcccgcg agccatttgc tcggcggcaa gaatttcatg ctcggcggca
    38341 gcaccgatct cacgaagctc gcgcccggca tcgaagcggg agatctttcg gggatcgtgg
    38401 cggccaggtt ttcacgctgg acacgcggct atatctatcc gccgcgtgcg ctggcactga
    38461 tcgccgattg ggatacgaag atcgacgcgc tgagccgcgg tatattggac gaggacatcc
    38521 gctccatttc aggcacgccg agctggctgc tgatcttctt cgagcggctg ttcgcgctca
    38581 gacccgagcg ggagccgaaa ttccacgcat ttttccccaa tctcgaattg atcgtccatg
    38641 gcggcgtgaa tttcgcgccc tatcgcgccc agttcgacac gcttctgcaa ggcacccatg
    38701 cggaaacccg cgaagtctat gccgccagcg aaggcttcat cgcgtccgcc gaccgcggtc
    38761 ctggagaagg catgcggttg gtcaccgaca atggattgtt cttcgaattc gtgcccgtgg
    38821 aggaattggc gtccgaaaat ccggcacggc attgggtggc gaatttcgaa cttggcgtgg
    38881 attacgccat cgttgttaca agctgtgccg ggctgttcgc ctatgtggtc ggcgacatcg
    38941 tgaaattcgt cgatcgttca ccgccgcgcc tcctggtgac ggggcgcacg tcctacacgc
    39001 tttcggcttt cggcgaacat ctgacgggcg atgaaatcga ggacgcggcc ggaatagcgg
    39061 cgaacgcaat cggcgccgcc atcgcggatt tttcggtggg cgccgttttt ccggacgcgc
    39121 atcatagccg cggcgtccat ctttacatcg tcgagttcag cggcctctcg cccaacatcg
    39181 acgccgcgcg gcacttcatc gatacaatcg acagcgtcct aagccggcgc aacgacgatt
    39241 atcgcgtcca ccgttcgggc ggattcgggc tcgacccgcc gcgcttgctc agcgtcaggc
    39301 gtggcacatt cgcggcatgg atgaaatccc gcggtcagct cggcgggcag cacaaggtgc
    39361 cgcgcattat cgccaacccg gaactattcc aatcgctacg ggattttgtt gaaaacgctt
    39421 tgtgaaaaag cctatgccgc gcgaaccggt ttggtggatt ttgcgcgccg cgtcggcgtg
    39481 ttttcacccg agagcaaata gcggcgataa tcgtcgagat cgccgtcaaa gggttcgacc
    39541 cggccatcgg cgaccaaaag gaagctgtcc gcggtggcct ccagtaagcg ccggtcatgg
    39601 ctgaccagga ttaccgcgcc gtcgaaatga ttcagcgcgt tcagcaattc ggaacgcgca
    39661 tcgatatcga gatgattggt gggttcgtcc agaatcagca tattgggctt gtccagcgtg
    39721 gcgagcgcga gcatcagccg cgcgcgttcg ccgcccgata gattcgcgac cttggtcgtc
    39781 gccttgtcgg cggaaaatcc gaagccgccc aattgcgcgc gcacctgctc catccgcaga
    39841 ttgcggcgca gatgcgccag cgtttcgata ggagtgaggg cgccatccaa ttcttccaat
    39901 tggtgctggg cgaaatagcc aggcacccat ttgcgcgccc gcaccagctc gccatccatc
    39961 aggggaagct tgtcggccag caatttggcc aatgtcgatt ttccgttgcc gttcttgccc
    40021 agaagcgcga tgcgatcatc gggatcgaat cgcatggaca tatgcgtgag gatcggcttg
    40081 ccggcttcat agcccaccga tgcgttatgc atggtaatga gcggcggact tgcctcgacc
    40141 ggtttgggga tgcggatggg cgcgacatgt tcatcgacgg gaagctcgat ggtctgcatc
    40201 ttggcaagca tcttcatgcg gctttgcgcc tggcgcgcct tggacgcctt ggctttgaag
    40261 cgatcgacaa aggcctgcat gtgttcgcgc gccgcttcct gcttcttggc aaaggccgca
    40321 tcgagtgcgc gcttggcggt gcgcgtggcg acaaacacat cgtagccgcc ggtataaagc
    40381 gtgagcttac cgcgttcgag gtgcaagatg aattcggcgg cggtgttcag gaggtctcgg
    40441 tcatggctga cgatcagaac cgttccgcga taacggcgca ggaaatcctc cagccacaaa
    40501 acgccctcga ggtcgagata gttggtgggc tcgtccagca acagcagatc ggggctcgcg
    40561 aacagaattg cggcgagcgc cacgcgcatc cgccaaccac cggaaaattc ggagcaggga
    40621 cgcaattgtt cttcggcgga gaaaccgaga ccggcaagaa tggaggccgc acgcgccggc
    40681 gcggaatatg cgtcgatatc gtcgaggcgc gtgtagatat agccgagcgc atgcggctcg
    40741 gtttccgtct ccgccgcgag caacagcgcg ctgcgctccg catcggccgc cagaacggtg
    40801 tccaggaggc tgtcggcacc gcccggagct tcctgcgcca cagcgccgac cttccattcg
    40861 cgaggccacg caatctcgcc gccatcgggc gcaagctggc ccaggatcac cttcagcagc
    40921 gtggatttgc ccgcaccgtt acggccgaca agaccgacgc gccgtccggg cggcagattg
    40981 gcggtcgctc ccgcgatgat ctcgcggccg gcgatgcgga cggtaacatt atcgatgctc
    41041 aacatggcgg caatggtcca aacaagagta cggcatcgcg gggtgtacgc ttcgcgattt
    41101 cgacgtgcgt cgatgtcaga aagaaaggtg acctaacggc ttttgcccgc caataccacc
    41161 ctattgtgcg acaaatcgtt aatcgagcgg catccggaag gtgaaccgcg tttccccggg
    41221 cgatgagtgg gccgtcagag tgccgccatg cgcgcgggcg atttccgaag cgatgtacag
    41281 gccaagtccc aggcctttct gattcggccg gacgtgaccg cgggaaaacg gttcgaacag
    41341 tcgttccatc gcggcgggcg gaatcggctc gccggcgttc gacaccgaaa gctcgaacat
    41401 gccgccgccg gtggtgccgc gcaccaccac cggagagccg ggcgttccat agctgagagc
    41461 attaccgacg aggttggaga gcaattgcgc cacacgcgtg cggtcgcatg tgacgggccg
    41521 gttcagatcg aatgcggttt cgacgacccg gtccggccat accgcgcgca attcgcccac
    41581 tacctgcgca aggaccggtt ccaatggctc cgaggaatcg cgtttcagcg cgaagcctcc
    41641 acccagccgc gcccgcgcca gatccagcac gttgtcgatc aggcccgaca tgcgagacac
    41701 gctgttctgc accagacgaa caatgttgac agctttctcg tccaatggaa tgcgcagcag
    41761 catcttcgcc ccggcatcga tcgccgccag aggattgcgc agatcatggc cgagaacggc
    41821 gatgaattgt tcgcgcagtt cagcctgcct gcgttcaccg cgcaaattgg catcgctaga
    41881 agccagtgtt tcgatcgcat cgagatgaaa gccgatgagt tcggcgaaga gcttgaaagt
    41941 ccccacgatt tccggcgtct tcaataggcg cggtcgcgga tcgatcgcgc acaacgtccc
    42001 gaaaaatcgt ccatcgcgca gcatgatcgg catcgagatg tagcttagaa agccatattt
    42061 ggccggcgtg tgatgcgtgc aataaacatc gtccgtggcg acattgtcga taacgatcgg
    42121 ctccttgtgc tgccggatct cgttgcagag cgtggtttct atctgcaatt cgtcgccggg
    42181 cttgaggccg aactggatat cgtccttcac gctgcaggcg atccagcggt cttcggtgac
    42241 ccgcgccacc gccgcgaaac ccatgccggt gagacggcac gcgacattga gaatgctggg
    42301 cacggccgct atctgattga tggcggcgat atcggcttga aaatcagacg tcattgtcga
    42361 accatcgtgc gtgcagagaa atccttatcg gaagcgcaag ccaccatgac ttccggccca
    42421 aggccactcg ggaagatcca ccattttata tacacatagg gcgtcgaagt ctcccggcgt
    42481 accgagtcgg tgtgcccccc ggatcgactt cgtgcgtatc ttgaaggtgg agcgtgtcgc
    42541 tcaatgcggc ccgcgcttca tgtacaattt caccgcgcca aggtccgccg attcaatcgc
    42601 caatatcgat tggccggtga aaattcttct cgatcgtggt gaaaccggac accagcttgc
    42661 cgctgtcgtt gcgattggcg tttgccatca cgaactgacc cggcttcacc tccgccatgc
    42721 tttcggtctc gttgcggtag tgcacgatgt tgtttgaaag ctcgacctgc attttgccgc
    42781 cgtttttctg caaatcgacg gtgaccttaa cgccatttgc ggttggctcg gccgcggtca
    42841 ccacgccgag atagcgcgtt gcattggggt tggttaccat cttgcgttgc tcaggcgcat
    42901 tggcgccatg cgcgaatgcc tgggtgtgcg cctgaattgg cttgcccatc tcttcgtcga
    42961 tcgcaatcac gtcccctgct ttgatggttc tcacgtcaag aggttcattg gagacgatgc
    43021 gcccctgcgg cgcgaggtcg aacgagatcg tatgaccgtc gcggttcgtc accgtaagcg
    43081 accgcccctc gacgcttacg atcggcccgt aaatgcgaac cggcacgttc tgggtcattg
    43141 ccggcacagc acagcacagt gccagcgctg caaacgcaca aactctgctc cacatgagcg
    43201 cttcctctat ttatcaaatt ccgatggaga ttagattttc acatcggcgg cttcaatccc
    43261 acgcagccgg cccatgcagc cgtaatgtct tggagccaca acagccgatc agcaacgctc
    43321 gccggcgtgc atcttaatct gtgtgcgggg tgcggtatat tgccggcaat ggtcattcac
    43381 gccggtgtgc cgttcgaccc ccggtgaatg cgagctttcc tgccagcact tcgccgctgg
    43441 cgaggcggct tgcggaatat gtcagaaatc cggcttgtcg gcggttggaa cgtgatagtc
    43501 ggtcgatgcg agattatttc tcgctcccac aaaattgcct tcctggcaga tgtcctcggc
    43561 catgcccaca tgcccctctt caacctcacc gcggtcctca tggtaggggt cgtagcgcct
    43621 gatgccttgc caaggttttt cgaaggtgcc cggatcctcc gccgtcacgt ggatttccag
    43681 cttcttgccg ccttcgatca gacgaatccg ttcgatgaca tgcagtttct cggtgtgcgg
    43741 ggtgcggaaa ttgtcggcaa aagtcttgtc gttcatgccg gtcgtgtcga cgacgagggt
    43801 gtcgccttcg taatggccaa ccgattcgcc gtagtaagtg ggtttcagat tggtgctatg
    43861 gggcacgttc aaataaacat gccgcgcgaa ctgttcgcct tgattggcga tcagcacctc
    43921 cttcggcgac tgaatgatga agaacgggcc gcccgagttc cagatcgccg gaacgccgta
    43981 gggccggcag gatgaacccg gtgtgtaggc gatctttccg gcaagcacct cctcattgtc
    44041 ctttttcata atgtccttgg cccactgttt gatattggga ttggtgaggt ccgagatgcg
    44101 gaaggtcggc tgcgtgccga ggcgctgggc gtccacgttg tgaatatagg ggtgcgtcgt
    44161 atcgtttccg gtgggttggg gcgcaccggg aactggaatg aaaatctcgc caccgcgagt
    44221 tctccagctc ctgccgtccg cggcaaaatt ggggattgca ctgggcgttt gcgccgccgc
    44281 gccggcaacg agactgacgg caagaacggc cgtgccgaca ccaaacaaca tcgaagcgaa
    44341 tttggacatg gtttgctcct tctgcctctc acgcgcaaac gtgcggatac gaaatacacg
    44401 gcagggcgcc gttcaaccgg cccgataccg cactttggtt cactccaaaa ccatccatag
    44461 gcgcccgaat ctcgaacggc cccctgcgca ctccaaatca aatattttcg ttccgtttga
    44521 gcctcgcatt accaggcctt ggtcaggatg agtccgaacg tgcgccccag atcgcttttc
    44581 gtaacgtcga aggccgcggg cgcggagccc gccgcgattt ggtattcgaa agagggcagc
    44641 ttgttcatga gattctggac tacgaactgg atgccgatat tcttgagata gtcattttgc
    44701 ggcaaatcgc ctgtcgtgta tccgatcgag aaatcgaatg tgtagtagga cggctccagg
    44761 cccgtgtaat tgctgatggc gcacggaagt gccccgccgc cgaccgtgcc gccggccgtg
    44821 aggcactgca aattgacatt gggcggcgcg ttttgggtgt tgtagtagtg tccggtgtaa
    44881 ttgacaaatc ccgtggctga ccaggggccg ttgctccagc ccacccggcc ccggtatcgc
    44941 atcctggggc ttgttgtaac gcccgcctgc gcaacaccgc cgacggtacc cgcgtccgtg
    45001 tggaaatcat caacgatcgc cggatttgga agttccggcg tcggattatt cacctcgtac
    45061 tcgtgcaggt aatatgtgcc ggttatgccc gcgttccacg cgccgagatc acccgcgtcg
    45121 tagtcatagc tggcgctgaa atcgatgccc tccagcttga tccaaccgaa atttccgatc
    45181 gcgccatcat tgatccaaag tatggaggtg agcgaacccg gcggcactgc gttctgggga
    45241 tcgttgagca cggcgctaac catcctttcg aactcgggac aggcagccgg cttgttgttg
    45301 ttgctgcatc cggccacgtc gatacccgcc ttggcgatat ccgtaggcac aatgtaagag
    45361 aaaccgagtg ccggattgtt gaccgtggaa ttgctcggat tgccgaactg cgtcagcgcg
    45421 ttgctgatct tcacctgata ccaggtcacc tggagatcga gccccttgag aaagttcgtc
    45481 ggggcgaatt ctcccgtgag cgtgtagttc gtcgcggtct cgggccgaag cgcggactga
    45541 gcggtattca cataggcgcg gaagccatcc ttgagcgcga cgccgccggt ggagtacgtg
    45601 cctaagccgc tgggattggc ccgagcgcca caaatcgtac ccgctgtgcc ggcgtttccg
    45661 acgacaccag cccaaccgac gtaacccgct ccgggattca acaacctgtc ggttagagaa
    45721 cccggtcccg cgctgcagtc gaggctgacg gtgcggtttg acacgaaatt cggaatattg
    45781 atgccggaga tgatgttgcc cacgatagcg gattgttcgg cgaaacttgg cgcgcggaag
    45841 gacgtgcccc aatcgccccg gacgaacagt cccatatccc gcgaaatcgt ccaattgaag
    45901 ccgattttgg gattactggt gccgccgacg tcgctgtact ggtcgtgacg ccaggaggct
    45961 tcgatttcca gtgcattgat gagcggaatc gaattggcct caccgacgat cgggatattg
    46021 agctgtgcga atatggccca gaattgcgtg cccttggatt cctgcaggag aggcgtcaac
    46081 agggacgcca ccttggtgtt gtcaagctgc gagtataccc atctttcgga ggtataggtg
    46141 gcgccgatgg cggctttgac atcgccgccg ggcagcgaaa acagcgggcc gtcaaaattg
    46201 acaccctttt cattgaacca gaaactcgtc gtagaaagtc ttcgacccgt gacgtaatcg
    46261 agcgttgact gcgcgttgca gtcgagcgag cgcggatcgc agaacaggtt cagataggga
    46321 agcgtcgcgg gcttggtcca ggtgccgatc gccggggccg ttcccgctgc aaatccggca
    46381 ggtatcgtcc aaccgagcgc ggccgatacg gcattcggat tgaccgcgtg ggtatcggta
    46441 cgcgtaccgt tgaaggtctc ggaataataa atgcgtcctt gccagtcgcc gggcaaatca
    46501 aaattcagtc cgagcaagta gcgatcggcg agttcactgg ccgtcgtgag gggcgggact
    46561 tccagtccga tattgtagtt gaccaccaga ttgttgggcg caccgccggt cggataataa
    46621 ggattgcggg tcggaacttg gacctgcaga tcctgagtcg aggccggcga ctgattggcg
    46681 agattgagaa attccgcccg tcgattgctg taataccctt cgccatagaa ggatatatct
    46741 ttggtgaggc gctgatcgac cgtcaacaca gcagcgttgt gctgctgggc cgcgtcgtac
    46801 cagccgatcg agtagggatt gatttcgttt gccgtgccgg ccgcggtatt ggtcgctccg
    46861 ccattggccg aagtgccgac gttcgcccag ttgataaggc cggggccgct gctgggcgca
    46921 agtggtccca gccccccgtt cagggaggaa ttgaaattgg cgccagcccc ggccggaagg
    46981 gcaaagcaat tggtacagcc cgttcccaac cctctgaggc tgctcggcgc cggaacgcca
    47041 accgagatcg tcgcggggat cgaagaggct atcggcgttc ggttgtcgag gccccacgga
    47101 ctgaaatcgg gcgtgaaatt cgaacggtcc ttgcccttga gcggcgaaga atccgaccac
    47161 tcgtaggtca gcgttacatc gccgccgtcc caggtccggc cccagagctg ggacgcctgg
    47221 tattgcgttt tgccgtccgc gacggtggcg cgaagctgtg tgatcgcacc gtcatacccc
    47281 cgcttaagga ggatgttcat cacgccgctg acggcatcgg aaccgtaggt ggccgacgca
    47341 ccgttcgcta gaatgtcgac gcgatcgatc gcgagcgaag gaatgatgga tggatcgcgc
    47401 tggccggagt gctcgccttg tggcggaacg cgataaccgt ccaccatcat cagagagcgg
    47461 acggggtatc ccccaaccga atcaagaccg cggagattta ccgaattctc cctggcgata
    47521 ttaccaccgt tgttcgtgcc aacggccccc ggcgtgacga caaacgcggg aatagtcttg
    47581 aaaaggtccg cggtcgtcag cgcgccgctc acaacaaaat cctgcggccc aacattggta
    47641 acagggacac cgactgaagc agcgccgcga attaacgagc cggtgacgag aacctgttcc
    47701 ggggcatctg ccggcccggc ctgagacgtt tgcgtctgcg cctgagcata ggctgcgccc
    47761 gagcttgcta tggccagcaa tgatgccgag cccagaagag atgcgatttt cgatactctc
    47821 ggatccatgc tccgtctccc ccgccttttc gttgaaaggc aaaaacatca cgaaacccgg
    47881 cgccctcggg gcagctggtg cgcggcaacg atattttggt tagtccccac ctcgggatca
    47941 cctgcccccc cgggcagata gactaccgcc gacgatgaaa ctgtcttgca acaggctgag
    48001 gaggtcaatc gatttaggag cccatattaa atccttttag gagccatatt aaaaatacac
    48061 tcatcagaaa cccgtattcc ggttgatact ggacaatcac gccggccgcc atcaaaccgg
    48121 atttctgaag cggaaaaaaa tcagccggat tagagcactg cggaagcgag ggagagattt
    48181 gatgaaacgg caggcactgt tcttcagcat cgttacggca gcaacgcttg ctttgcccgc
    48241 atttgccgcc gatgagggta ttaccggcgt ccagtggggc agaaattcca aagatttcga
    48301 cggcatgcca tccggaccga aaccgctgag aaatttgaac cgcgatgcaa acggcgtggc
    48361 cgacgaccat gcactggtag gcgattatcg caatccgatt ttgacgccgt acgccgccgc
    48421 gattgtgaag caaaaaggcg aggaagcact tgcggcgggc tttaccacgc cgaaggaaca
    48481 gtgccgtccg ttagcgccgc ccttcagcgc ggccatacag ttcgatttcc agatcctgcg
    48541 taagaaaagt ggcgacctga cgattgttta tcacgcagac aaccaggttc ggcacattcg
    48601 catgaacggc acgcacccag cgaaggtggc gcctacgccg atgggcgatt cgatcggtca
    48661 ttgggaaggc gatgcgctgg tcgtcgatac cgttggggtc aagtccgata agttcacaat
    48721 ggtggaccgt tttggcatac cgctgacgga ggcgatgcat gtcgtggagc gttatcggcc
    48781 gatcgacggg gcgcgcgcca aggcggatct gaccaaatat gaggagagcg agggcgtcgt
    48841 gggtggcaga accaatggct acaatcccga caccaagctg acagggctgc ggctcgaaat
    48901 cacgatggaa gacccgaagg tgtttactca gcctttgacc ggcgtcgtca cctaccggcg
    48961 cctgacgttc ggttggcggg aggacgtctg cgccgacaat ccggtcgaac attacaaaga
    49021 cgagtggatc ggtctgccca ccgcgggcca cccggatttc tgagctgaaa acctgtcatc
    49081 gttcacaccg cgcaatcgtg cttccttgct ggatccactg ctccattgtc gcagcgtgct
    49141 gccggtgagg attgatgccg atcaaattgc cggacgtagc gatgtggcaa acggcgacgt
    49201 gtaccgatga cgaactcgcg gctataaaac accatcgtca ttcataattt aggagtcttc
    49261 ttgcaatgag tgccaaagtt gttcacgtcg cgattgcgtc ggatcattac gcgatcaacg
    49321 cgcgcttcta cgaatccctc ttcggcatga agccgtcgag caaaccgagg ccgtcacgct
    49381 ccgttgtcgt cggagatggc tatgtcgggc tgaattgcat tccgcggcgt gaagggcgct
    49441 gttcggggat cgatcatttc gggatcgagg tggacgatct ggaagatacg atcgcgcgca
    49501 tccacaagtt cgatcccaca ctggaggcgg tgaagcggcc cccgatccgc ccgttcgcgg
    49561 cttacagtgc gcatgatcca gactgcaacg tgttcgatct gtcgcagcgg aatattggcg
    49621 tccagaaaga cgtctatgcc gaaaacgacg gcgagacgcg gccgcgccgc atcagccata
    49681 ttgcgattcg tacgcggcag gcagaaaaat gcgcgcagtt ctatgcagac gtgttcgggt
    49741 tagcgccgca gaaccgtcag gacgagaatt tctacctcag cgacgggcgc gtcacgctga
    49801 tgatcattcc gtggaagatc ggcgattatt atgggcagga ccccgcgcgc acagggctgg
    49861 accatatcgg attcaaagtg gaatcggtcg agcaggtgaa gaaggatatg gaattcctga
    49921 taggcgagaa tccgctcatg catgggcggc ccctgggcta tggcccggaa ggcgagtccc
    49981 ggctgaagct gttccaaaaa tgcccgctcg gttgcttcca tctgaccgat attgaaggcg
    50041 cgctcatcga cgtgggggag tgagcgaatt ttgcgttaaa atgttcggcc cggtgtaccg
    50101 ctgggccggt ttgcgccggc gcgcagacct cgctttgaac gcgggaggcc gcgcgcgatc
    50161 actgtagtcg cttcagattt ctccctggcg gccggcgcgg ttcattccat tccaacagaa
    50221 cgtccaagaa tgtacggctc tgcgcgttat tttctggtca gctcttcata ggttgtttcg
    50281 gtgaacgtct ggaaccgatt ggctgacttt agcggctctc cgaaagcctg cttgatatca
    50341 tcgagcgatt tgccttgttt cacgagatcg gcgatccgtg cgcgtttcgc ttccgcattc
    50401 gcaagactct ggcgcacctg ctctctcgtt ccagcgcccg cgtggccgcg aacatacgta
    50461 tcggcatcga gatccacgag cgcggagacg aatttgatcc agccttctga gctgccgttc
    50521 ttgaaaaggt gaatggtcgg atcgccgtcg ccgatgagat cgccgctggc gacgagctta
    50581 taatcgggaa aatagacagc aagatcgccg ctcgtgtggg cgggcgcgat atggatcagc
    50641 gtcattttaa caccctcgac ggtgatggat tcgcgcattt gcgtgatgat tttgttgggc
    50701 aggtattcct tgggaggcga ttgaagccca cccgcagcca aatccttttc gatttccttt
    50761 ttatcgttct cgtgagcgat gacggtcaaa tttaaaccct tgggaaagcc ccctaggccg
    50821 cgcacatggt ctggatcgct gtgggtgagg ataaccgtcg taatgggttt attcgtcagc
    50881 tttccaactt catcgacgag ttgcttgcca tccggcggcc tgttttttgc gtcaacgacg
    50941 ataacgccat tctggccgat gatgatggtg gacgcgccgc cgccgccctc ggccatgtaa
    51001 atgttgtctt tgagcttcgt tacggtcagt cccggatcgg gcacctgagc aaatgccggc
    51061 ataccgaaaa aagccaatac cgtaacggtg aatagaccgg ccgttcgagt tcgcatgatg
    51121 gactccattt tgagttgctt cagctggcgc atttcgtccg gcaacattgt ccttcaaaaa
    51181 tcaggctttg atgctgtcgg taagcgaaag aactttcccg taacgggatt gttcgccgct
    51241 tcggcgcagg ctgcttccgc aagcgggtcg gaagctcgtc tgtattttgt aatggcggac
    51301 catgtggccg tgaatgcttt cggatcgtca taggtcaatg ttccctcgag ggtcttgcca
    51361 tccgaaagaa ctttatatcg ctcgatcaga tggagatcgg agctgtgcgg cgttccgtag
    51421 cggtcgatga gggatcggtc gttgaagcca atcgtgtcga taacgagcgt atcgccttca
    51481 tagcggccaa ccgattcgcc ataccaagat ggtgccggat ttttcgtgtg cgatcgattc
    51541 agatagacgc gccggacctc gtggtcgcgc gcatatacga tatagacagt ccgctgtgtc
    51601 tgaatgaaat agtgttggtc ggcccacaac accgccgcag gcccgccagg ccagcaattg
    51661 ctctgagccc aaaacggatc ttcacctcgg atagcggttt ccgcttcgct ttttaggatg
    51721 cccgcggccc atggcgtaag aatgggactt cggtaatcgc cgatccaagg cctcgaggtg
    51781 ttcctgcgat gcgctgcgtc gacgccaaat ccaaaatgcg aataccccgc cagatcgcct
    51841 atcggccccg ggccagcctc cgggtccagc aggccagtcg catttgtgct ttcccagctt
    51901 ccggaaaaat cgggataatc gggggagggg ctttgcgctg ccgccgcact cacagccaag
    51961 agcacgacca ggcccaaaag aaatacccgt tttgatgtct taggtttcat ggccgccccc
    52021 ggtgccaata gcattggtgt cgcgcgcaac ggagcgtccg cgtcgcatat tcattcggtt
    52081 agaaatccgg atggttcgcc atgggcaggc cgatccactc atctttgtaa tgctcgattg
    52141 gattgtcggc gcagacgtat tcccgccaac cgaaggtcag gggcaggtag gtgacgacgg
    52201 cggtcaaagg ctgggtaaac accttcgggt cctccatcgt gacttccagc cgcaatccct
    52261 tccgattggg atcgggggtg tagccattgg ttctgccacc gatgacgccc tcggtttttt
    52321 catacgcggc gatatccgcc ttggcacggg cgccctcgat caaccgatag cgctccacga
    52381 catgcatcaa ttcgctctgc ggtgtgccaa aaagatccga cgatatgaac gcgtcggtct
    52441 ttatcccaac ggtatcgatg accagcatgt cgccttccca atgaccgatc gaatcgccca
    52501 ttgccgaagg cgccagtttc gcggggtgcg tgccgttcat ccggatgtga cgaacctggt
    52561 tgtctacgtg atacacaatg gtcaggtcgc catccttctt cggcaggata tgaaaatcga
    52621 attgaacggc ggcggtcagt ggcggcgcta tcgtgcggca ctgatcctca gaagtggaaa
    52681 agcctccctc agcaatcgct tgttcgccct ttttcttcaa agccgcggct gcttccggcg
    52741 tcaaaatcgg attgcgataa tcgcccacca tttggctgct gttggccacg ccatcgagat
    52801 ggcggctcaa atttttcacg ggttgcggtc cggatggcat gcccccgaaa ttgtaggaat
    52861 tcctgcccca ttggacgcct gtgataccgt caccggcggc aaatgcgggc acagcaaaca
    52921 ttgccgctgt gaggatgttg aagaacagcg cctgccgttt catcgcaccc ccctcttggg
    52981 atcgcccttc ccgtgcagca gattgtctgc cgctgccaat cagactgtct ggcaataatt
    53041 agaggagatc aatcaattta ggagatatat caaaattttt taggagatat gttaaagcca
    53101 ttcgctcata agcgggtggc tcgccgttca tgcggcaata aaatctgtcg cagcgaacac
    53161 tccggacaaa aataacgctg caaccgcctt tccggaaaaa tcccgattta aaaatctggc
    53221 gtgccggcag ttggaaccgc gacgtctttc tcagtgccgt atttgcgcgg gttctcagcg
    53281 caaacgtcct ctccccagat ctgcggattc acgcgctcat atgtaatcgt cgcggaccag
    53341 ggtgtggtga acacaacggg atcctcgacc gtaaaccgca gctgcagata ctggctggtt
    53401 tcattgaatt tgccgtcaaa atcggggatg acattttcct ttgcgctgcg ttccaatccc
    53461 tctttcgcgg ccgcgtaatc cagcagccta tatcgctcga tgacatgcag cgccgggctg
    53521 tgcggcgtgc cgtaccagtc taccatggca taagggccaa ccttcacgcc gaccgtatcg
    53581 atgaccagcg tgtcgccttc gtagtgaccg acggaatcgc cataccagga tggcgtcacg
    53641 cgcgccggat gagactgatt catgcggacg tggcgaatct ggtgatcgcc gttatagaga
    53701 atcaggatct cggtcggtgt ctgaagcatc tgaatttcat tgttggtgaa ttcgaagggc
    53761 acgcctccgg gccaacattg gttgcgtggc gtcggatagc cgtaatggtc cagcgacatt
    53821 tcgccgaacg tcttcacgag cgccgccgtc tccggcctca gaataggatt gcggtaatcg
    53881 ccgacgagtt gcctgttgtt gctgacattc cgttcgcgtc ggtgcaggtt gaccagcgac
    53941 gtggaacccg aggccaatgg ttcgaaaccg gggattccct ggtggttata tgtaccggag
    54001 aaatccggaa taggcgcggt ttccccaacg ccagacgccg cgccgaacgt tccgaggatc
    54061 acgagcagaa ggaaattcca cggctgcgca ttcatggttc gttccagctt ttatgcgtga
    54121 tgcgtgtatt agcccgtgcc acagatgacg ttgagcagcg cctgttggcc gcccgccact
    54181 gcatcaagcg cgcgatcgag cgctttagga agcgcggctg cattttccac acgttcgcca
    54241 taaccgccga ccgtctcgat ggtcttctca tagcgcgtgc cgggctcgaa ataggtcagc
    54301 ggttcatgat tgctcttggc cgcatagccg tccgggtaga catcgcgcgt gttacggcgg
    54361 accgcattcc acatctcgtt gttatagatc acggtcaggg tcggaagctt ttcggccttg
    54421 ccgacataat gggcggcaat cggattgccg aacatatagg agccgtcacc gaccgcgcag
    54481 atcacgagtc tgtccggcat cgccgctttc atcccgagag cggtaccgag gccccaaccg
    54541 agcgcaccac cctgccccag cgaataaaaa gtacccggct tgttgaaatg cagatgctcc
    54601 gaaggagtca gggcttcctt gacaacaatg ccatcctcgc ctttcacccg atcgatgcaa
    54661 tgattgatcc agccggggtg gatggtggag gcgtttttcg ccttttccag gttggctgca
    54721 atgcgcgtat gcaacgtgtc gcgcgcttcc gccaccttgc accggcgtgc ctcgatccgc
    54781 gatgaggccg ccttttcgcg cgttgccaat gcgtccgtca gtgcgggaag gcttgcgcca
    54841 agcacgccgg tgatcgccag atcgcagggg aagccgcgca atggataatt ctcgaagagt
    54901 ggatcggcgc cgatatggat gatcttgcaa tcagggcgcg gcgccttgcg cttgggtacc
    54961 caaggcacat cggcttccag caccacgata acgtcggcgg cgtcgaaata gctttccgga
    55021 ttcgacccga gatgcatcgg gctgttccat ggcatggaca tgtaatgatg cttgcgctcg
    55081 aacacgggaa tagcgaagcg ctccgcaacg gctgccaatt tcgccacatc ggtttccacg
    55141 cggcctgcgg cgcgcgtgat gatgagtgga aattccgccg ccgccagcat atcggctgcg
    55201 cggtcgatgg cgttcttgtc gggaaatgcc ggcgagggcg tcgcgtgacg cgacggcgaa
    55261 gcgtaacgga aattcttaat cggcgcggca agcgtctcgc gcggcagcgt caaataaacc
    55321 ggtcccatcg gctcgctcat ggcgatgttc agcgcgcggt cgaccgctgc ttccatcgtc
    55381 tcgccattgg gcagctcgta gtcccatttg acgaattcgc gcaccatggc gcgctgatcg
    55441 cgcatttcct gcggccaatg gatttcgccg ccgcgcatgc ccatcaaccc gccttcttcg
    55501 gaatagggcg tgcggccggc ggtgaacagc acaggaatat tgccgcgcca cccgttgatg
    55561 atgccgcaga gcgcattggc cgtgccgacg ttcacatgca ccatgacggc ctgcgggcgg
    55621 ccggtcttga ggtaatagcc ctgcgccatg gcgatggcga cgttttcatg cggcacggtg
    55681 atgggcttgg gcactttcgt gccctccact tgtgctttgg cataagcctc gatgatcggc
    55741 gcgaaatccg tgccggcatt ggcgaataga caatcgatgc cgcggtccgc cagcagcgca
    55801 agataagcgt cggcgaccgt tcccgactcg atgatcttta tggacacaaa ggactccgct
    55861 ttagtttttc aggaacggca cggcgaggcg gatgaattca tccgccgcat cccacggcac
    55921 aagatgttta cagccattga caatgtgaac atcgagctgc gggaattttt ccttcagcag
    55981 cgtggcgcgc tcggctgctt tggcgcggtc ttcgcggccg aagatcatca gcagcggcat
    56041 cttcaactcg atgaggcgct gccacagcgg catcttcggc tgcttcggcg gcaatacttc
    56101 ggcggcctga ttgcgcgcga caaaagcctg gaaattccta ccgatgctgc gcgcgtgccg
    56161 aagcgccaat tcctccggcg tgatcagctc gtgatgaaac aaattcgctt cgagcagctt
    56221 gcgcgtatcg tcaatcgccg gctcgctctg agccatgcgc cgctccagcc tctgctgcgc
    56281 agcaccttta ttgccctcgc cttcgccgtc aaccggcggc aaaaggctgc ccgttccgag
    56341 cacgacgata tgcgaatagc gggacggatc ggtgagcgcg agctgcaccg cgggattgcc
    56401 ggcttgcgaa tgacctgcga gcgctgcctg cttcaggccc aatgcctcca taaacttcgg
    56461 aatactgtcg cgacgatagg cgggcgacag gtcggtgggt acatcggaaa ggccaaaacc
    56521 tggctgatcg aaggcgatgg cgcggaagcc cgcttgggcc agcggcgcaa gattgcggat
    56581 gaacacatcc gccgacgaac ccaaggatgc gccatgcaga agaagaacgg ccggtccttc
    56641 gccttcttcg atatatcgca gggcgaggcc atcgaccgtc acgaatttat cttgcacgcg
    56701 agtcacgcgg tcggtgctca atgctccact cccttttcgg acatcgatca cgccacggcg
    56761 cggcggcctt gtggctccgc cgcacctcgc gatgtcttgg cgcgcacatt ggcgaggatc
    56821 aggatccaca cgcagccgaa gaacatcggt atcgagacca gatagtacat cgtctgcagc
    56881 ggccaatgca gcgccagcat cgtgccggcg aacatgacgc tgattatcga gccgacgcgg
    56941 gcgacgccat gcgcccaact cacgccggtc gagcgaatat cggtgggata gagtgttgca
    57001 gccagcgcgg ccagtgtaat ctgggtgccg atgttgaaga agcccgaggc gaagattgtc
    57061 gcggcgagcg gatagaatgc cccgcccgcc catccgatag tcgcgatgaa cacgccccct
    57121 gccgcgaaag aaccggccag caccttgaaa aagccgaagc ggtcggagag aaagccgagc
    57181 gtgatcacgc ccagaatgcc gccgaattgc agcgaggcgg cggcgcgcaa agcctgcgct
    57241 tcaggcagac cggtttcggt ggtcaacgtc ggcagccaat tgttcagata attcagcgcc
    57301 agagtgttca tgaacaggca cagccagatc agaaccgtgg cgctggtgcg cccttccatg
    57361 aagagacgca ccagcctcgg ctgattggcg ctttgttcgc gcagttcctg gcgcaacacg
    57421 aaatgcgtgc ccggttcgaa ggtcagttcc ggagcgatct tgcgcaaaat ggcgatcacg
    57481 ccatcgctgc gcccctgcac ggtcaaaagc cggattgatt ccggcagcca aatccatagc
    57541 acccccgcca tgacgaacgt catagaggcc gcggcataaa attgcgcctg ccagccgaaa
    57601 gccggaacca gatgggacgc gagcaaaccg cccgaggccg agcccatcgc atagccgcag
    57661 cccatcagcg tgaccatggc ggcgcgcctg gcttggggcg caaattcatt cacgatcacc
    57721 acggcaagcg gcaccgccgc gccaaggcca agccccgtga gaaagcgcaa gcccatcagc
    57781 acgttcaaat tcgatgcgtc gggaatcgca ggcaacaaca gggttgaaat ctccggcact
    57841 tgcgtggcga taagcgagaa caacgcgaca taaatcacag tcagtaccgt aacccgtttg
    57901 cgcccatagc ggtcggctag gggcccaatc acgagtgcac caacagccac accaagaaca
    57961 cctgaggaga ataccgtgcg cagcgcgccg cgggccaaat cccagtcatg gctgagcgcg
    58021 ggcgcgatat aagcgatgga ctggttgtca acgccgtcaa gaaacaaagt gagcccggcc
    58081 atgaaggcta tgaggcactg ataacgactg actttatgag aatcgatgac gtccgcgaca
    58141 tcgaccgtcc cggtcatggc tcccccaatg catctttagc gtattgagca agctacacaa
    58201 aaaaaaacgc tctgtctcag cgatgccgtt tcagtataac tcctataccg aaagggtctt
    58261 gtgcttgcgg cggacctcaa agacggcatc ttctctggcg gctcgtccaa tgcgacaatc
    58321 gttacatccc gccggtaaaa acaatctgtg agaccatgag gatcgcgtgg atatccggaa
    58381 gctgatgtat ttcgttgctg tgagcgaaca gaaaagcttc acaaaggccg cagagattct
    58441 gcggatcgcg cagcctgccc tcgggctcca gattcgccag ttggaacaac aactgaaggt
    58501 tcagcttctc gtgcgtcatt cccgcggcgc ggaattgacg gacgcaggcg ttattctgct
    58561 gcaacatgcg cgtgacatcc tcgaacgaat cgaactggcg ggtgctgcga tacgcgattc
    58621 gacgtctacg cagggcgagc gcattatcat cggcatggcg ccgagcattt cggcgatgct
    58681 gtcgtatcct ttgatcaaat tgggcgcgca gaagcttccc aatgtcacca tcgttctcgt
    58741 cgaggaactc agttccgtgc ttctcgaatg gttagagaat ggccggatcg atattgcgat
    58801 cgggtacgaa ctgctgcatt cggccaaggt tgcaggcgat ccattattcg tccaggattt
    58861 ctttctcgtg cagtcaccca aagcaaagac tcggcgaagg aacctcaatt tcgccgagct
    58921 tgccgatatc gagcttttaa tgcccgcgag tccgcatggg ctgcgcgaac ttatggaagc
    58981 aggcgcagcg cggctgggga tccatctgaa aatccgcttc gaagtacaat ccatttcggt
    59041 gatgaaagag ctggtggaac agggaatttg cgccgcggtc ctttcctatg gcgcagttgc
    59101 acgcgaatgc gagaaaggcc ggctcgtcgc aaccgaaatc gtcaatccga ctgtcaagcg
    59161 gatcgcctat cttgcctatt ccctgcgcag gccgcggtcc aaagcaaagg acgcagtatt
    59221 ggcatttatc cgcgagttgg cgctgaagca aaagggcgtc aattccgatc ttcacagtat
    59281 ggctccatcg tccggacggg cgactaaaga tttaggcgcg caaaaaaaac atcggcgaaa
    59341 cggcgtgacg aagccgcgta cagtcctaac gagttccggg gggccagcat ttggtgggcg
    59401 aaagacctag catgatcgcg gttgccgccc aagtaagcgc cctcttatga gtgtattcga
    59461 tatagctccc aaaatttttt gtgcgtttgc cgggtctgtg gcaaacccaa ccatcgggat
    59521 agttccttcg acgtctacgt gcagcacaat aactttatcg gggagggctc aatgccagcg
    59581 gcggttttga gaaatgagtg gaagccggtc catgccagga tgcttatcgg cggagaatgg
    59641 accatcagta cgcagcggat cgaggtgttc agtcccgccc ggcccgatga attggtgggc
    59701 acagttccgc gcggcacgcc ggcggatgtg gagcgcgcaa ttgcggccgc aaaggcggcg
    59761 caacccgttt gggcttcgaa gagctatagc gagcgggcac ggattattgc ccccctgctc
    59821 gaacggttgg ccgatgatat cgatgagcgc accgttctct atgtgcgcga gaacggcaag
    59881 accaccccgg aagccagggg cgagcttgcc ggcgttcctt cacgccagcg cctcacgctt
    59941 gacctggcag cggaattgga tcagcggcgt gaagccgatg cgcctaatgg caaaagcttt
    60001 gtcggctatc ttccatttgg cgttgtcgta tccatcgtgc cttggaatgc gcccatgggt
    60061 ctcgcgtttc ttcagataat tcccgccttg cttgccggaa acacagtcgt gctgaagccg
    60121 ccagagacct gtccgttgtc gctcatccgt gcgatcgaga tgatcgcgcc gatgcttcct
    60181 ccgggggtta tcaatgtggt gaccgggttg ccaaccgaga tcggcgatac gctcacaacg
    60241 catccagatg tggacaaggt cgcgtttacg ggcagtgtgg cggctgcgcg taaaatcatg
    60301 gccaatgccg cactgacgat caaaggcatt accctggagc tcggcggcaa cgaccccgcg
    60361 attttgttgg aagatgccga tcttggcgat gccgcgatgg ggcgcatggc gaattcggtg
    60421 taccgcatga cgggtcaggt gtgcatggcg atcaaacgga tctacgttcc ggaatccatc
    60481 aagggcagat tcctcgacgc tttcacccgg actgtcgaca agatcgtggc cggcgatggc
    60541 ctggacacga ccgtgaccat ggggccgatg cataccgcgc gggccctgga gcgggcgcag
    60601 gccatcgttg ccgatgcggc caagcgaggc gcaaaagtcc agatcttggg ccaggttccg
    60661 gataaagcag cattcggcca cgggcatttc atgcagccta ccgtcgtcag cgacattgcc
    60721 gaagacgcgc ccttggtggt ggaggagcaa ttctgtccgg cggtacctgt cgcgacatat
    60781 cgcgatgtgg acgacgccat cgcccgtgca aatgcaacaa tctatggttt tggcgggtcg
    60841 atctggagcc gggatatcgc acgcgccacc gatcttgcgc ggcaactgca tgcgggaacg
    60901 gtgttcgtca acacgcatgg aaccgaaagc gtcaatcgca agctgcccta tggcggaatc
    60961 aaacacagcg gtatcggacg gcgcgcgggg atcgagggcg tcagagagta tatgcaagtt
    61021 cagacattga cgacgttcga gcaatgaatt gcatgctcag actccattgg agatcgcgat
    61081 aggcgaggaa agggacaccg ccgcggactc ggacacgcat gagaccgctc agctgctggc
    61141 cgaagcgatt cgcctgcatc ggtcggggcg gatggccgac gctgcgcgcc tctgtgagcg
    61201 cgtgcgtcag cacgcgccgg acaatttgac ggcattgctg ctatcgggaa cgatcgagct
    61261 gcagtctggc cgacatcatg cggccgacca atatttccag cttgcgcaag cggccgcacc
    61321 gcactcagtc gacgcggcca ccgcgcggtc gaacacgctc agagcgatcg gcaaacatga
    61381 tcaggccctt gctgtactcg aggatattct ccggcacagc cccgatcacg caatcgcatg
    61441 gaacaatcgg ggtaatctgc tgctggaaat ggggcgaaac actaatgcga tcgagagtta
    61501 tgaccgcgcg atttcgcttc atcccaatta cctcgacgca ttgcacaatc gtggcgtcgc
    61561 acgaatggct tcaggcgaca attttgcggc ggaagccgac ctcacgcggg ctctggagct
    61621 gaaaccggat tacgtccctg cccttgtgaa ccgcggaacg gcgcgcgtcc agcaaagtcc
    61681 ggagcgcgca catgacgcga tcacggacct tcggcaggcc gtcctgttgg agagaacaaa
    61741 ccccgatgcg tggcatatgc tgggccgaag ctatcttgcg ctcggacagc atgcggaagc
    61801 cttgtccaat tggtccacgg ggctggcgcg gcacgcgaac catgcggcga tgcgacacga
    61861 cagggctttt ttattcaacg cgctcggccg ctattccgat gccgctaccg atttcgaacg
    61921 gctgattgcg ctgaacctcc atgatgcggc ggcatggcag ggacgcggaa tcgcgctggc
    61981 gcggctgaat catgaaaaag aggcgctcga gagcttttcg caagcgctga gattcaggcc
    62041 cgacgatgtt gtgtctctgt acaatcgcgc ggcgattttt tccaggttga aacgctatga
    62101 ggaagccgct tccgatctgg aggttctggt cgctgcggat cctgaatttc cgctcgcccg
    62161 cgggctgttg ctgagcgcgc gactgtatct atccgactgg aagggactgg aacggcaacg
    62221 cgaggccatc gatgcctgcg tgcggcgcgg ttcgagggtg atccatccat ttgcacatct
    62281 tctgatatcg gattgctcag ccgcgcagct cgcttgcgcg cggcggcaca cctctggcgc
    62341 gtatcccacc gcgcgcgtgc cgctctatcg cggtgagcaa tacagccatg aaaaaattcg
    62401 cgtggcatat ttttcgggtg atttttacca gcacgccgtc ccatctctga ttgcgggcgt
    62461 gctggaaaat cacgattcca gccggtttga gattcttggg atttcctacg gcggtaacga
    62521 caagagcccg atgcgcgcgc gcttggagaa agcatgcgcc cgctttttcg acgtgcgcga
    62581 acgcgatgat gcatcgatcg cgacactgct ccggaatctg gaagtcgata tgatcatcga
    62641 cctgaagggc cacaccggcc ccgaacgtcc tggaatactc gctcaccggc cttgtccggt
    62701 gcaggtgaat tatctcggtt atcccggaac catgggggcc gattatatgg attatctcat
    62761 cgccgaccgg atcgtaatcc cgccggaaca gcggcagttt tattccgaac agatcgtcta
    62821 cctgcccgat acgtatcagg caaatgattc cgcgcgcccg atagcgccgg acggtattac
    62881 ccgcggcgat gcaggcctgc cggaggcggc atttgtattt tgttgcttca atggcagtca
    62941 gaaaattctg ccgccagtat ttgattcctg gatgcggatt ttgtcgcact ccgaaaacag
    63001 tgtcctttgg ttgcttgagg acaatcaagg cacggccgtg aatcttcgtc gtgaggcgca
    63061 ggcgcgaggt attgcagcag agcgattgat ttttgcccgc cacgagcggc ctgaccgcca
    63121 cctcgcccgg ctcaaattgg ctgatctcgt tgtggatacg ctgccctatg gagcgcacac
    63181 aacggccagc gatgcgctgt gggccggagt accggtgctg acctgcatcg gatcaagttt
    63241 tgccgggcgt gtcgccgcaa gccttctcac ggccgccggc ctgcctgaac tgatcatgca
    63301 atcggttgtt gaatatgaag caacggcgca aaatttcgcc gctacgccgg gactgttgtt
    63361 gaaaatccgg gaaaagctgg cgcgcaaccg cgaaagctgc gcgttgttcg atacggttcg
    63421 tatgacgcgg aacctcgaag ccgcttatgt gaaaatgtgg cagcgccacc agcgcggcga
    63481 atctcccacg ggctttgcgt taccctgacg caatccggtg gccgtcaaaa acgccggcaa
    63541 tcgcagcaat cacgaatgac gcgatggcgt ccgttcgatc aggggagagc aagccgaaat
    63601 ggctcctata ttcttttgtt tgtggtgcgc agagcccgca tcatggagga tcggctttcg
    63661 catgtacctt cgggaggggt tcgatggaag aatttcgcgt tcacgctcgc gctggggtcg
    63721 ccatcgcggc tgcgttggct ttttcggcga tagccggttc ggcctatggc gcgggcgcca
    63781 cgccggacat ttccggcacc tactggacgc cgcgctatta cgccacgctt ccgatcgtag
    63841 gcggcggtgc cctgcccctc accgcgatgg gcaaggaggc ctatgccaag aatcaagccg
    63901 gactgaagag cggtgaaatc accgacgcag cgcgcaaatt ctgcgtgcct gacgggatgc
    63961 cgcgcgcgat ggcaagtccc tatccgttcg agatcattca agatccggcc aaccaaatca
    64021 ccatggtcca cgaactcaat caccagattc gcgtgattat gatggacaag ccgctgcaga
    64081 cttacgagca agtcgttgcc tttccctatt acaatggcca ttcggtaggc cattgggaag
    64141 gcgacacgct ggtggtccag accaacggct tcaacgagaa gacctttctc gatgcgaccg
    64201 gcgcgccgca ctcggacatg ctggtaacca cggagcggat ccgcaagacg ggcgcgaacc
    64261 agctcgagga tgtgataacg atccacgatc ccgactatta cacgcgcgat ttccaggcgc
    64321 gctttaccta tgtgctccgc aacgacgtcc atctcgagga ttatgtctgc ggcgaaccgc
    64381 atcgcaacat cgacatgatt cccggcatcc ccgaagctcg gcgggccaat gccgcagccg
    64441 gtcgatagcg caacaagcct ctgtgtctct tcatcagccg ccggatcgca tccggcggct
    64501 tcgtcttttt tagaccctgc cacaaccctc ctcactgccc gcgtttgtct ttggacaagg
    64561 ggcacgccgt tcatgaatct tatagctgcg ttcacctttt tatatgaccg gcggctgcgt
    64621 cggcggatta aagtcgactg cgatgcggcc acggttttcg gtatccccgc gtgccactaa
    64681 cgaggagata aggatgaaat ttaaagcact tgggttggcg gcagcggcgc ttggcctgta
    64741 tgcgatcccg gcactcgcac atcattcctt cgcgatgttc gacgcggaca agaagatcgc
    64801 gctggaaggc acggtaaagg aattccagtg gaccaacccg catagctgga tcttgatcat
    64861 ggttcccaat gcgcagggca cccccgaaca atgggcgatc gaaatgggtg cgccgggtgg
    64921 tctggcgcgc caaggctggg tcccgaagac cttgaccccg ggcatgaagg tgacggccac
    64981 gattcatccg cttaaggacg gcacgcatgg cgggcagttc atggcggtgg cgcttccgga
    65041 cggaacgctg aagggcaacc cgaacggcgc agccggcgcc aacgcgggcg cgaattaata
    65101 tcgcttattt cgg
//
LOCUS       DASYEG010000117         4774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1920730, whole genome shotgun sequence.
ACCESSION   DASYEG010000117 DASYEG010000000
VERSION     DASYEG010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1920730"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VGM72_04920"
     CDS             <1..310
                     /locus_tag="VGM72_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264638.1"
                     /translation="LSCDSVTSDKTYKNSVDSGSGTSETAPQLICHATYTVSNFTSGS
                     ITTLDFMDWPETIGIDNLTVTLPEARSIAVFALGLLGLGLIVRRKRGRWTLSAGAKEA
                     "
     gene            complement(384..3230)
                     /gene="prsT"
                     /locus_tag="VGM72_04925"
     CDS             complement(384..3230)
                     /gene="prsT"
                     /locus_tag="VGM72_04925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HEY4264639.1"
                     /translation="MSGKNGSRKTRRYALATLWMLGVPTGIYGGNSPAEAAVPIPNTE
                     AASPMSLDTKKLLDDAQSAQSRGELGLAIIQLKNAVRLTPRNGEVRARLGMALLKSGD
                     TRTAERELRQARKDNAPEDLVVPAILDAMIVRGEARELLEEFADPSLQTQSKIAADIL
                     RARALALQIVGRSADANAAMARSLSLRRDTRGLIAKAKLAMQQRDFALARTAIDEAAK
                     LSPSNEEVLLSQIALLFQSGDAKKTLAAADDFIRRTPNSTIGRVMRIEALLALKQDKT
                     ASDDVDTLEMAVPESSFIPYYRGLIFARAGDYKDAWHLVETLHPEFILAQPAMAMLVA
                     EIAIASGNIESGGAILATLVSRRPELTAARVRLAGVQLQLKSPTAALKTLEPIKTNAD
                     PEIQALFGQASLQLGRFSDAIGSLEKAIDASSAKNTDALQLDLAESAFGYGDIDRAIR
                     ALEQIKAHGPTDWQTAIPLIASLVQAGKYQEAVEVVDGIAKSAGRTPLVPFYRARVLA
                     AASDLAGASAAFGEALAIDPKFVPALYFRAHVLVARGNPEAGKKDLRQVLALDPRNVP
                     AYIALAQISLHEGEESQSVGLLNSAIRVAPNDPTPRLTLATYQTSQGKYQDARATVNA
                     LLKIAPNDPATLMQLGQIQFLSGDTGKAIETFRSLAATYTTAAGTYVVLAKVLHATKD
                     RLAAIDAAKRAVELDPFSPKIRGLLVEYLIAGDRPDEAIANAREYVSSHPGTDADLLL
                     AAALIGVKRQEEASAYLTTRLAAKPDRLLALRLSQLAMDMGNRKKALDILAEWLRKSP
                     GDYDVGRQYGASLLQIGDVPAARKEFETLLMRRPEDPVVLNDLGWILRDDDPARALSL
                     VTLAVKIVPDSTQILDTLAWIKFQRRDLQGALLLLRRAHSLDTGDGEVGFHFAVALDA
                     TGRRAEAKTLLQSVVEKNLEFTDKDNAKKLLARW"
     gene            3469..4311
                     /locus_tag="VGM72_04930"
     CDS             3469..4311
                     /locus_tag="VGM72_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY4264640.1"
                     /translation="MRTTATGLSLVAGACAMVTACAAQLPPAPLAPVSAPVASTPPAE
                     DSQSREARERYVVHALSTHAPPGVEGMRRIGSGSGFFIAMDRVVTNFHVVESCRAMTV
                     GNNTEGEEVDAKVIATDPRADLAVLAAEEKDIQPAQFLAGPFAETREDLAIVGYPEHG
                     LVVLQAELHRVAVFQDDLERGGARYNFFGPVRRGNSGGPLLDAHGAVVGVVTAKIDTV
                     ALYKKTGEVVDDLGVAIANHTVFDFLSKNQIAFAPATADADLSSEDLLKKAHGFVRQI
                     GCWR"
     gene            4535..>4774
                     /locus_tag="VGM72_04935"
     CDS             4535..>4774
                     /locus_tag="VGM72_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264641.1"
                     /translation="MSESPRLIDALNSRWKDRSAWRSSIAFRLAWRNITRDYLRLAIA
                     VIGVGFAVLLMMVQSGLLIGFATTTSSLVDRAGADL"
BASE COUNT          928 a         1465 c         1377 g         1004 t
ORIGIN      
        1 acttagctgt gactccgtca cttcggacaa gacctacaaa aattcggtcg attccggatc
       61 gggaacgagt gaaactgcgc cacaacttat ctgtcacgcc acctatacgg tgagcaattt
      121 cacatccggc agcatcacga ccctggactt catggattgg ccggaaacca tcggcatcga
      181 caatctgacg gttacattac cggaagcccg cagcatcgcg gtgttcgcgc tcgggcttct
      241 gggattgggt ctcatcgtgc gccgcaagcg cggccgttgg accctctctg ccggcgccaa
      301 agaggcctaa gtccgcgccg acggacggct ctcctttaga agagccgtcc gaataccgcg
      361 ttcaatggcg agaattacga aacttaccag cgcgcgagca atttcttcgc gttatctttg
      421 tcggtgaatt caagattttt ctcgacgacg gactgcagca gggtcttcgc ttcggctcgc
      481 cttccggtcg catccaaagc cacggcgaaa tgaaatccga cctctccatc gcccgtatcc
      541 aggctatggg cgcgtcgaag cagcagcaac gcaccttgaa gatcgcgccg ctgaaacttt
      601 atccatgcaa gcgtgtccag gatttgtgtg gaatcgggca cgatcttcac cgccaatgtt
      661 accagggata gcgcccgcgc cgggtcgtcg tctctgagaa tccagccgag atcgttcaag
      721 accacgggat cttccggcct gcgcatcaac agagtttcaa attccttgcg ggccgccggc
      781 acgtcgccaa tttgcagcaa cgatgcgcca tattgccggc ccacgtcata atcgccggga
      841 ctctttcgca gccactccgc gagaatatcg agcgccttct tccggttgcc catatccatg
      901 gcaagctgac tcaatcgcaa cgccagaagg cgatccggtt tggcggcgag gcgcgtcgtc
      961 agataggcgc ttgcctcttc ctgacgcttc actccaatca gtgccgcggc gagcagcaag
     1021 tccgcgtcgg tccccgggtg agacgacacg tattcgcgcg cgttcgcgat agcctcgtcg
     1081 ggcctgtccc cagctatcag atattccacc aggagcccgc gaattttcgg cgagaaggga
     1141 tcgagttcca cggctctttt cgcggcatcg atcgccgcga ggcggtcttt ggtggcatgc
     1201 agcaccttgg cgagaaccac ataagttccc gccgctgtcg tgtaggtggc cgccagcgat
     1261 cgaaatgtct cgattgcctt gccggtatcg ccactcagga actgaatctg ccccaattgc
     1321 atcagggtcg ccggatcgtt gggagcgatt ttcagcagcg cattcaccgt cgcccgcgcg
     1381 tcctgatatt ttccctgact ggtttggtag gtcgccagcg tcaagcgcgg cgtagggtcg
     1441 ttcggcgcga cacggatcgc gctgttgagc agccccacgg actgggactc ttctccctca
     1501 tgcaacgaaa tttgcgccag ggcgatatag gcaggaacat tgcgcgggtc gagcgcgaga
     1561 acctgtcgaa gatccttttt tcccgcctcg gggtttccgc gcgccacaag aacatgggcg
     1621 cggaaataaa gtgcaggcac gaatttggga tcgatggcaa gagcctcgcc gaacgccgcc
     1681 gaggcacccg ccagatcgct cgccgcggcc agcactctgg cccgataaaa cggcaccaat
     1741 ggggtgcgcc cggcgctctt ggcgatgcca tccacgacct cgacggcttc ctggtatttg
     1801 cctgcctgca ctagagaggc gataagcggg atcgctgttt gccaatccgt tggcccatgc
     1861 gccttgattt gctccagcgc gcgaatggct cgatcgatgt cgccatatcc gaacgccgat
     1921 tccgcaagat cgagctggag tgcgtccgta ttcttggccg aactcgcgtc gatcgctttc
     1981 tccaacgaac cgatggcgtc gctaaaacgg ccaagctgca agctggcctg accgaacaag
     2041 gcctgaatct caggatcggc attggtcttg atgggctcca gcgtcttgag agcagcggtt
     2101 gggcttttca actgcagttg cacgccagcg agacgaaccc gggccgccgt tagttcggga
     2161 cgccgcgaga cgagggtcgc cagaatggct cctccgcttt caatgtttcc gctggcgatg
     2221 gcaatttcgg ccaccagcat cgccatggcc ggctgcgcca aaatgaattc gggatgcagc
     2281 gtctcgacca ggtgccaggc gtccttgtag tcgccggcgc gcgcgaatat caggccgcgg
     2341 taatacggga tgaaagagga ttccggcacc gccatttcca gcgtatcgac atcgtccgaa
     2401 gccgtcttgt cctgcttcaa agcgagcaac gcttcgatgc gcatcacccg gccgatggtg
     2461 ctgttcggtg tacgccggat gaaatcatcg gccgcggcca gtgtcttctt cgcatcgccg
     2521 ctctggaaca gcagcgcaat ttgcgaaagg agaacctcct cattcgacgg cgacaatttg
     2581 gccgcttcgt cgatggccgt gcgcgcaagc gcaaaatcgc gctgctgcat tgccagcttg
     2641 gccttcgcga tcagacctcg agtgtcgcgg cgcaggctta gcgaacgcgc cattgccgcg
     2701 ttggcatctg cggagcggcc aacgatctgc aaggccaagg cgcgggcccg caggatatcc
     2761 gcggcgatct tgctttgcgt ttgaagagat ggatcggcga attcctccag caattcacgc
     2821 gcctcgccac gcacaatcat tgcgtccagt atcgcaggta caaccagatc ttcgggcgca
     2881 ttgtccttcc gggcttgacg cagctcgcgt tcggcggtgc gtgtgtcgcc gcttttcaga
     2941 agcgccatcc ccaatcgcgc gcggacctcg ccattgcgcg gcgtcaaacg gacggcgttc
     3001 ttaagctgaa tgatcgccaa cccaagctca ccgcgcgatt gcgcgctctg agcatcgtcc
     3061 aggagtttct tggtatccaa actcataggc gatgccgctt ccgtgttcgg aatcggaaca
     3121 gcagcctcgg caggggagtt ccctccatag atgcctgtcg ggacgcccaa catccagagt
     3181 gtcgcgagcg cgtaacgccg ggtttttcgc gaaccgttct tgccgctcac gataccttcc
     3241 ttgcatcccc gctgcgctca gagcgcgttt ttcgtaaccg gagtattgag attttgcaga
     3301 ttcctataca agagcctgac ggggcttcac aattaacatt accgagcgag gttaacggaa
     3361 gctttcactc tttgtcgtga caggtcacat cgcccattcg caatagcgga ccgattgcag
     3421 cccacgggca agacctttta atatctacgg tgtcggagga gaaccacgtt gcggaccaca
     3481 gcgacaggtt tgtcattggt tgcgggcgct tgtgccatgg tcacggcttg cgccgcacaa
     3541 ctgccccccg ccccgttagc gccggtgtcg gccccggtcg ccagcacgcc gcccgccgag
     3601 gactcccaat cgcgcgaagc ccgggagcgc tatgtcgtcc acgcgctttc cacccatgcc
     3661 ccacctgggg tcgagggaat gcgacgcatt ggatcgggaa gcggtttctt catcgccatg
     3721 gaccgggtgg tcaccaattt ccacgtcgtc gaaagctgcc gcgccatgac cgttggaaac
     3781 aacaccgaag gtgaggaagt cgacgctaaa gtgatcgcca cagatccacg agcggacctt
     3841 gcggtgctcg cagcggagga gaaagatatc cagcccgcac aattcctggc gggccctttc
     3901 gccgagactc gcgaggatct ggcgatcgtc ggatatcccg aacacggcct cgtggtgctg
     3961 caagccgaat tgcatcgcgt cgccgtcttt caagacgatc tggagagagg tggtgcacgg
     4021 tacaatttct ttggcccggt gcgcaggggc aatagcggtg gtcctctgct cgacgctcat
     4081 ggcgcggttg tgggagtcgt gaccgcgaaa atcgacacgg tcgctctcta taagaagacc
     4141 ggcgaagtcg tcgacgattt gggggttgct atcgcaaatc acacggtatt cgattttctg
     4201 tcgaaaaatc agattgcctt tgcgcctgcg actgcagacg cggatttgtc gtccgaagac
     4261 cttctgaaga aagcccatgg attcgtgcgt cagatcggct gttggcgata acgcctcccc
     4321 gcatgtgagc tttgcccgca tgtgagcctt gtccgctgaa cgcatcctta ccgcttggcg
     4381 ccgattgcgc gcgcatgaac tatttcttca ccaagattgt acgccgtctc cttgcgcctc
     4441 cgctgcaaca cccacgcatg ggacggagaa acttagcgtt aatgccgcag gcgctattta
     4501 tccgatgttc catctgggat ttccgcaaag cgccatgtcc gaatcgccgc gcctcatcga
     4561 cgctctgaat agccgctgga aagatcggag cgcatggcga agctcgatcg cgtttcggct
     4621 cgcatggcgg aacatcactc gcgattatct gcgtctggcg attgccgtga tcggcgtggg
     4681 atttgccgtc cttctcatga tggtgcagtc cggacttctc atcgggttcg cgaccacgac
     4741 gtccagcctg gtggatagag caggagccga tctt
//
LOCUS       DASYEG010000118        23962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3370524, whole genome shotgun sequence.
ACCESSION   DASYEG010000118 DASYEG010000000
VERSION     DASYEG010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 23962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23962
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3370524"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..128
                     /locus_tag="VGM72_04940"
     CDS             <1..128
                     /locus_tag="VGM72_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEY4264642.1"
                     /translation="KIENYFCRIKDWRRIATRYDKLARNYLAAAAIVGALVWIKL"
     gene            1875..3941
                     /locus_tag="VGM72_04945"
     CDS             1875..3941
                     /locus_tag="VGM72_04945"
                     /inference="COORDINATES: protein motif:HMM:NF014581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEY4264643.1"
                     /translation="MTRFRIKAHARARAAAHIEHSYKILSAALTALLMSTRIASAQNF
                     DSFMDQYFGYGVFGALVFWWWVRRGKNKRVARDQQQASEQTEEFRRNFPERNSREALA
                     ALGATKGERSQRIEDIFNRKSESLTFEMQDMNPRQETGVVVITGTLVRAIAAMAPRER
                     ESLRAAYHLFNINEFDPKGFCHVRLALVFGDGKAQHANVNFYAGDNLKQNQNVHTVAD
                     AIKYWTGMNAEGAVSAVLHRLQAVATKMPHDPTVKQLTGRLLGGGDQALSSTCEFAQA
                     QGGKEGAGLILGIDEETGQQVVFAGEGSVITVAPPGSGKTQCHVFPTLLSWKGPAVVL
                     DVKGEIYAATSKWRSENVGPVYKFSPLAPAASHGYNPLTPIRSDPDYLWEDSRFLADM
                     MLVPTGGKDPFWENRARDVLTAAIARVCLNPDVSKRSLGDVLDVFHGVGWDQFVRALQ
                     MAVDIRSMTRAGSSLGEMERKTRDGVLQTGLSSLSAWDGDRIERATRKSDWSPLDLRN
                     AKSPTIYICLKPNEVESYISVLRVFIAQHIRMLTSSLPPRGAPSILFVLDELPRLRQM
                     PPVEEALEIGRQYGIKLWMFTQSLGQLENAYPNAEGMVGNCALRMYMNPSLHDETAQK
                     ISDDIGFQEGVLDNARQKIVEAPVLAGPDYKDYVIVMASGFKPFRVRKSFAYQDAMLK
                     SRMGSL"
     gene            3982..4845
                     /locus_tag="VGM72_04950"
     CDS             3982..4845
                     /locus_tag="VGM72_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264644.1"
                     /translation="MPESDYIDRQAARLHQERRSAEAALDRVDDARQSLNEQPSSVEV
                     ANRQAELESEQKLAEAELNRIEADLRKIEALEPEDRAKEATLETDAKLKGQIEKADAL
                     KIEAAEEDRRKEEFFAKEQREKQEHFEDQTRMKEGLERTEREGIEAIEQQTKDASEKS
                     AEENVISEIASDLASDGLKIADTLLEIAESAKLELEKSAMAERHKEELEQLRRDHEQA
                     QTELVARLQQRQADNSEIDRACQALVKAAERILAELLQRQEEERQRLAEQRAREAQQR
                     AQRAASDGLSL"
     gene            4842..4952
                     /locus_tag="VGM72_04955"
                     /pseudo
     CDS             4842..4952
                     /locus_tag="VGM72_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611359.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5206..5733)
                     /locus_tag="VGM72_04960"
     CDS             complement(5206..5733)
                     /locus_tag="VGM72_04960"
                     /inference="COORDINATES: protein motif:HMM:NF027788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HEY4264645.1"
                     /translation="MKYWIDTEFIAQPYTIDLISIGLVAEDGREFYAESSEVDWTKAN
                     QWILNSVRPQLEGIGISREEIGYAVRRFIDEDKHPVFWGYFPAYDWVVFSWLFGGMTE
                     LPFSFPQLCLDIKQWAIELGDPELPHQMSARHHALADARWTKDAWAFLAGLHPEASER
                     RSFGHKYPELTGKPL"
     gene            complement(5819..6196)
                     /locus_tag="VGM72_04965"
     CDS             complement(5819..6196)
                     /locus_tag="VGM72_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY4264646.1"
                     /translation="MMASRVFAVFTMAMSLAAGALTDGALAADAGKPDPVKGRALYLA
                     NGCSYCHGTVGQGGGGRTGGLRLAQMGIGYEAFLNQLRHPVNEMPPYAQSAMPDSDVA
                     NIHAYIASLPPPPDVKSIPALNR"
     gene            complement(6207..8150)
                     /locus_tag="VGM72_04970"
     CDS             complement(6207..8150)
                     /locus_tag="VGM72_04970"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY4264647.1"
                     /translation="MTGKADLPSAKRRKFLKGAGFAGAAAFATPLNAVAQPAVRPGPA
                     NNPVPLPNKAAETAVPPELEVLTEGKSGSDFMVDCIKSLGFEYMAANPASSFRGLHES
                     LVNYGGNANPEFLTCSHEEISVAMGHGYYKIEGKPLCVFAHGTVGLHHATMALYNAWC
                     DRVPVYVVIGNIMDAQLRRPGIEWNHSAQDPAAVVRDFTKWDDNPASLQHFAESSVRA
                     YKIAMTPPTLPVLLVADGALQEDPIPDGLDLRIPKLPKISPPQGESGAVAETARLLAT
                     AENPVLIADRFARTSQGSKMLVELAEALNCPVIDTSGRLNLPSRHPLNQSSRARAHLS
                     QADVVLGLEMTDYWGTINSYRDQLHRTSKPITKPGAKLISLGSGDLYTKSNYQDFQRF
                     ADVDMAVTGDGEATMPMLIEAARTHIDGTKKSAFETRGKKFAEMHKADFERARVDATY
                     GWDASPVSTGRLCAEVWNQIKDEDWSMASPTASLSYWPQRLWTMDKPYHFIGDGGGMG
                     IGYCASASVGAALANRKHGRFTVAIQCDGDLLMTIGVLWTAAHHKIPLMFVMHNNRGY
                     HQELMHVQRMANRHMRGLDRAHIGTTLRDPYIDYAKIAQGMGIAAIGPITDPKDLEPA
                     LTRAKAIVKGGEPVLVDVVTQGR"
     gene            8303..9070
                     /locus_tag="VGM72_04975"
     CDS             8303..9070
                     /locus_tag="VGM72_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264648.1"
                     /translation="MTRIISAAVLVLGLGTMTLAFAQTPLEQSAEHRFQLDFHVSDTA
                     LQKMLPQGWAPAVAAAGPAKDANLRMIFIDRIGIVGTDGKPLGRGAARMVYLAVPVKQ
                     TAGDTAGQMIVAGLTDNAADAPGAFGVYQYAATAKMTRSVNASNGTAIAEEDWDFVGN
                     GGEHMQVHVKYERAPAAKGGGEVKFFNPADPSKYQIFKTEQTTDITRNATTNPPDRVK
                     EFSYRAGGGRIASLFDGTEKVLSWDSQPWYNRSVIAP"
     gene            9103..10455
                     /locus_tag="VGM72_04980"
     CDS             9103..10455
                     /locus_tag="VGM72_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264649.1"
                     /translation="MRHLRLPHVGLALGLVVGTSVAAQETAKPGLPDTIPCPEAVAQI
                     ATCYSAKLDSGAYVLAAMPKHWNGNLIVFAHGGPSLVPATATSSKGDLSKYSVEVERG
                     FGWIASSYRREGYGIQMAVSDTEDARKFFIARIAKPKRTIIHGASYGGLVAAKLVETV
                     AKDADGRPLYDGAFLNSGLVSGSAVGYEFRADLRVVYQYYCKNLPRANEPQYPLWLGL
                     PADSKLTLKEMESRVDECTGVSKPVTERTERQKQNLANIIGVMGFSDRLLTRHMQSAT
                     YIFRDIATRITQGKSAFTNIGIVYRGSSDDAALNHEIVRFNAAPEARAALRADGQPTG
                     ALPIPMVAIHSINDPQVAVEVESAYRDAVQGAGNAERLVQAYTDENQHTGQSAPEIAA
                     ALEMLMQWIEMDYKPDASIIAEACAQLRSGFDGPCRYHPEFQPKAYGTKFYTRDVVAR
                     "
     gene            complement(10452..11729)
                     /locus_tag="VGM72_04985"
     CDS             complement(10452..11729)
                     /locus_tag="VGM72_04985"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4264650.1"
                     /translation="MTEDQIFARIARRLLPFVMLLYVVNYIDRVNVGFAALTMNKDLG
                     LSPAVFGFGASIFFVGYMIFQVPANLMLEKLGARRWIFLILAVWGLLSACNAFMQGPR
                     SYYTLRLLLGIAEAGFFPGMLLYMTYWFPQSWRSRFVGVFMAAIPLANIIGGPLSTSI
                     LGLDGIAGLHGWQWMFLLEGLPATALAFLSLKLLPDRPAEARFLTDEEKRIVTARLKK
                     DEGSSEHRDFWPALRDPRVIALGLVLLGNQIGLYGVQLWLPQIVKDMGFSNFATGFVV
                     ALCFIASMVAMILWARHSDATEERIWHVAVPLLLGAFGLMFAALVQSNMLVLLSLAVA
                     LVGTLAYNGPFFALPSTFLAGTAAAGGVGFVNTIGSLGRFIGPYGVGVLKESSGGYSS
                     AMAAMGAAMMISGLIVLVMGRKMGARKVAVKTV"
     gene            11914..13137
                     /locus_tag="VGM72_04990"
     CDS             11914..13137
                     /locus_tag="VGM72_04990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEY4264651.1"
                     /translation="MKKVVVTGMGCVSGLGPDRASAWKTLMAGEGGIRPVVKFAQGQQ
                     EMRIEGPGAAVPPDAAKRLNAHFTEKQLAGVDPFSTFAAAATLEALEDAGLWGTSASL
                     GEAAIIYGSASGGNLAVEAGYVRMFLQGMPNVHPMTIPRCMSSASVAHLSMLFGIHGH
                     SLAVSSACSSSAHAIGEAMHLIRAGRASIVVTGGSEASLTFGSLHSWKALQAMAPDAC
                     RPFSMDRKGMVLGEGAATLILEEEGHALARGAHIHAVVAGSGSTSDASHITQPDAESA
                     AAAIRAAHRDADLSGDAPILISAHGTGTRLNDRSEASALRLAYPSSLDRHRVIATKSA
                     HGHMLGATGAMEFLLAIEALNAQIAPPILGYLGPDPECDLPLVLAPEKIACEATVSAS
                     FAFGGLNCVLIAKKK"
     gene            13184..13435
                     /locus_tag="VGM72_04995"
     CDS             13184..13435
                     /locus_tag="VGM72_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEY4264652.1"
                     /translation="MDLEQELRAVLKTKLPPGAEIDMNTKLADAGLDSLDVVEIAFDV
                     EDKFRIQLPPLAGQITDVTFLDLYRLVEEKIAAKAAETI"
     gene            13512..14585
                     /locus_tag="VGM72_05000"
     CDS             13512..14585
                     /locus_tag="VGM72_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HEY4264653.1"
                     /translation="MTEAHINRIATAVPPYDVHQAFVQFGRSLLTHDERRSALFERMV
                     DRAEIDHRYSYLSPNERADQGGMSAIDFYTRKSFPSTGARMKLFEDQAPMLAEIAIGR
                     LNLRPEERARLTHILISCCTGFSAPGLDLEIMKRCGLPGSMERSIIGFMGCYAAINTL
                     KLARHIVRSEPHARVLVINLELCSLHLKETSNLDEILSFLIFGDGCAASIVTAEPVGL
                     ALDSFRAVLVPDTHDLITWHIGDTGFDMILSGQVPGAILEALRKGAGEILDGAPVSAF
                     DLWAVHPGGRSVLDAVERAFDLRSNTLSASRSILRRFGNMSSATVMFVLETMLRASAQ
                     TRGCAMSFGPGLIAETMLFHGTG"
     gene            14608..15327
                     /locus_tag="VGM72_05005"
     CDS             14608..15327
                     /locus_tag="VGM72_05005"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY4264654.1"
                     /translation="MALLRTMEPEWLDEIPADDFRAIRSRRDLKRINAFMLQTAILAR
                     LLTRHQTTPPRRIIELGSGDGTFMLGVARRLASVWPEVHVVFLDRQNIVSDETRENFR
                     ALNWQTESVSADVFDFLSAQKPGSADIVTANLFLHHFSEQALSRLFAFAAQLAPLFVA
                     CEPRRSSSALIASHMVIALGCNDVSRHDAVVSVRAGFSGQELSKLWPQDRGWNLQEQP
                     ALPFTHCFAARRIERAGHADV"
     gene            15314..16501
                     /locus_tag="VGM72_05010"
     CDS             15314..16501
                     /locus_tag="VGM72_05010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY4264655.1"
                     /translation="MPMYDALIIGAGPAGSTAARLLAKAGWSVALVEKSEFPRRKVCG
                     EFISATSLPLLYDPHVRESFLSEAGPEVRRVGLFAADAMLTSAMPAADASRTGWGRAL
                     GRDRLDLLLVQGAVAAGAEVFQPWKVVVLQRGPGEFTCTIAAGDTRQDLCARIVIAAN
                     GSWEKSSWETTRPRNRAASELLGFKAHFSDSALPSDVMPLLVFPGGYGGMVHTDGGRV
                     SLSCCVRRDTLDACRRAHRGLPAGDAVLQHIMHHCAGVADALLRARIDGGWLSAGPIR
                     PGIRPRYAGGIFFVGNSAGEAHPVIAEGISMAMQSAWLLCQRLMAAEDRTAISASTSE
                     IGRLYAKDWHRAFAGRIRAAAMFAHLAMWPSAARALPVVKRFPEILTWGARLSGKATQ
                     LVP"
     gene            16562..20185
                     /locus_tag="VGM72_05015"
     CDS             16562..20185
                     /locus_tag="VGM72_05015"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY4264656.1"
                     /translation="MAANGTWEFWIDRGGTFTDIVGRTPDGALVVHKLLSENPEQYDD
                     AATAGIRALMMRHGEAPIAAVKMGTTIATNALLERKGEPTVLAITAGHRDALRIGYQS
                     RPNLFARHIVLPEPLYTQIIEIDERVTAEGEILKPLDEAAARCAFEAVFVDGYRAIAI
                     VLMHGYRHTAHEKRLESIARAVGFTQISVSHEVAALIKLVPRGDTSVTDAYLSPLLLR
                     YVDRIRGRLDEKTRLFFMQSSGGLAEANAFRGKDAVLSGPAGGVIGLAKVVAAAGFAK
                     AIGFDMGGTSTDVSHYAGIYEHSRGNAIAGVRLHVPMLDIRTVAAGGGSICRFDGLRL
                     RVGPDSASAIPGPACYGRAGPLTVTDCNLLLGKLQPEFFPRVFGCTGDGPLERGVVAR
                     KFAELAVEITASTGKSITPQEIAEGFLRIAVANMAKAIKQISIQRGHDLAEYTLVAFG
                     GAAGQHACLVADALGMKRVMIHPLAGVLSAYGIGRAELRTIREQTMNVRLEAAVDRLG
                     AVVEVLLQDARESLVRQGAAPTAIQGFARVELRYQGVDSTLSVPLQGIAQMRAAFENE
                     HRRRFGFASAGKSLIVESVSVEAVEFATTSEEPASRPQTVLASPLVQVRACLGGEERE
                     TQVYDRDAMLAGVEIAGPAIVRETTATTLIEPGWRARVDPLRNLILERAHPSPAYSTI
                     GTAADPVSLELFNNLFMAVAEEMGLSLQNTASSVNIKERLDFSCALFAPDGSLIANAP
                     HIPVHLGSMSDSVRTIIAARGFVSDGYPRDGRGIKDGDVYVLNAPYRGGSHLPDVTVI
                     MAIFDDGWRIAGFVAARGHHADIGGINPSSMPPFSRTVEEEGVLLDNVLLVDEGRFRE
                     EEMRALLASGRYPARDPDSNIADLKAQVAACVKGAAELRALIGHYGRDVVHAYMVHVQ
                     DNAAECVRRVIDTLANGSFSCEMDNGAVIRVAIIVDRASRHARIDFTGTSAQRDDNFN
                     APLTVCRAAVLYVFRTLVTDDIPLNEGCLRPLELVVPEGCLLNPNYPAAVVAGNVETS
                     QAITDTLYGALGIQAAAQGTMNNFTFGDGARQYYETICGGSGAGPDHHGTSAVHTHMT
                     NTRLTDPEILETRFPVLVEEFAIRKGSGGGGRFHGGDGVVRKIIFREPMTASILSNRR
                     RVAPFGLHGGAPALSGRNRVLRADGTVEDFGACATTAMQPGDVFIIETPGGGGFGPRA
                     STE"
     gene            20194..21225
                     /locus_tag="VGM72_05020"
     CDS             20194..21225
                     /locus_tag="VGM72_05020"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HEY4264657.1"
                     /translation="MRPGSVREEFPRLDACTASRLAAIALGHVTREYPNRLDHMLEGP
                     ADLESPQALHPVFYGSFDWHSCVHSYWLLATLIRLFPEMPEASAIRALFNNTFTKKNI
                     AGECTYLERKSAALFERPYGWAWLLMLQAELLRHGGIEGRRWASALRPLSAMFVARFL
                     AHLAKATYPHRAGTHGNSAFALALALEYARTARDKGLAMLIRKTARRFYAKDADCQAW
                     EPSGEDFLSPALMEVYCMSRVLPAQRFRAWLSRFLPRLAAQNPASLFSPARVSDRTDG
                     RIVHLDGLNLSRAWCWRAIAGTLPENDRAGPVAARAARRHFAAAEGFLTGHYMGEHWL
                     ASFALLALR"
     gene            21241..21768
                     /locus_tag="VGM72_05025"
     CDS             21241..21768
                     /locus_tag="VGM72_05025"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEY4264658.1"
                     /translation="MNSFYTIGHSTRSLDAFADLLVSSGVKELVDVRTIPRSRTNPQF
                     NADVLPESLRPFGIVYRHIAQLGGLRGRRKDRPQSPNTFWENSSFRNYADYAGTEPFR
                     EGLSNLREIGHRQTSAIMCAEAVWWRCHRRIIADYLLSLGETVFHILGPNKIEPAHLT
                     PAAQQAADGTLLYPK"
     gene            complement(21773..22540)
                     /gene="madM"
                     /locus_tag="VGM72_05030"
     CDS             complement(21773..22540)
                     /gene="madM"
                     /locus_tag="VGM72_05030"
                     /inference="COORDINATES: protein motif:HMM:NF015757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005342 - organic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015849 - organic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate transporter subunit MadM"
                     /protein_id="HEY4264659.1"
                     /translation="MTDILKRLADEHALIVAFAAVGGVMWLSYAVSKYLTFGRIHGSA
                     IAIILGLVLAYAGGIATGGKNGLADIPMFAGLGIMGGAMIRDFAIVATAFEVDVNEAK
                     KAGLLGAIALTLGTILPFIVGTSVARAFGYSDAVSMTTIGAGAITYIVGPVTGSALGA
                     SSPVMALSIATGVFKAVLVMTGTPFVARSIGLDNPRSAMAFGGLMGTVSGVTGGLAAT
                     DPRLVPYGALTATFHTGLGCLVAPSILFFIVRAIMGS"
     gene            complement(22537..22959)
                     /gene="madL"
                     /locus_tag="VGM72_05035"
     CDS             complement(22537..22959)
                     /gene="madL"
                     /locus_tag="VGM72_05035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005342 - organic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015849 - organic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate transporter subunit MadL"
                     /protein_id="HEY4264660.1"
                     /translation="MTIFGVALLAICTLAGALLGDVLGVALGAKTNVGGVGIAMLLLI
                     AARAWLMQRGKLNHGFKLGIEFWAALYIPIVVAMASIQNVVAAVRGGPVVLLAGGGGV
                     LVSLAATALLSRWSRPRDAVPASAEEAHAARVLSGAAE"
     gene            23120..>23962
                     /locus_tag="VGM72_05040"
     CDS             23120..>23962
                     /locus_tag="VGM72_05040"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY4264661.1"
                     /translation="MGSEVAASALPLAGLIVAEFCNVAAGPFCGMLLADMGADVLKIE
                     PPGGDMLRQWPPHTNGFSENFASLNRGKRSIALDLKNPADNEIALRIMGRADVVIENN
                     RPGVMERLGLGWLRIHAEKPKLVYCSISAYGQSGPRAGQGGFDVTMQAASGLMSVTGE
                     TEGAPVKCGVPVADFTAGLYGAFAIAAAIARVRAGGEGLYIDVPMLGTTLAIGALQTS
                     EYFGTGRDPQALGSAHPRNAPYQAYRARDRYFVIAAGNDKLWASVCDIVERPGLVSDP
                     RFATV"
BASE COUNT         4838 a         7043 c         7287 g         4794 t
ORIGIN      
        1 acaagatcga gaactacttc tgccgcatca aggattggag gcgcattgca acccggtatg
       61 acaaactcgc cagaaattac cttgccgccg ctgccatcgt cggcgcgctc gtctggatca
      121 agttgtgagt ccagacccta agtccgctca aggcggcata cttcaagaat gcagtatcga
      181 taagcttgta gaacatcact tctcacaaat ggaatccgct tcattccgat tccttgaaga
      241 gcccagaact caaatttcca gacgcttatc tactggcatg tacaaagaat aacggaatgg
      301 tacgcttttg ttcgtacccg gtgcatcatg ctccagacgg ttgcgcggcg catcccgaga
      361 tcgcgggcga cttggtagga aaagcgtacc attccgaaaa cattatatgg cggctcaagc
      421 cacgaacacc acttgccgct atccgacatt ctgagggcgg atagtgacat gcattcatcg
      481 gcccgattga ttcagaaacc attggactgg cgatccaatg atcctgcgtg ctcgcttggc
      541 tgaaatgaaa ttcagatggg cgcttgctgt tgttcgtaag agtttcagat ggcttggatg
      601 aaaacatcga catctgaatg cgcttaggaa atcgataggg gatctatgca aatttgtttt
      661 tgctggatta gctcatggaa atgcatggcg gatggggatt actagatgcc tcgcatagga
      721 gatggggatt gctacatacc tatataaagc ggcgaggggc gatttcgctt ccaattgatg
      781 atggctagaa tttgagaagt acctttgatt gcgtggaatg ttgggccttc tggctataac
      841 gaccgccgac tttcttttcg catcttttgc aacttctatc ctaaagcacc gaagtacgtg
      901 tcgattcttc gtttctacga gcggtctgcc aggaccatac ggcgcgtgaa tgaccgactt
      961 tggatctccg cgatcatgaa tttcccacga gatcgtcaca gggaagtcac tcgacttgcg
     1021 cggcgctact tccacaatct acttggggcg gcgtgatgaa agtcgaggac gacatgcgaa
     1081 catacgaaat tcgctacgtg gacgacaatg acaatctcat tggcgcaatc ttcacgacac
     1141 agagggacgg tacggatgcg tccatttacg cacatgcgat gacattgcgc tcttacaaga
     1201 gaatggaagt gtgggacgcg gactctctca tctatgagcg gccctgcaga tcgaaacctc
     1261 attagccaag ttgatagggc tgggaacagc cgactttgac tgcgggttca acgggtcact
     1321 gcaacaccag ccgttagaat gcggctggtg ttgcagtgac ccgttgaacc cgcaccgtaa
     1381 agcagacgtt ccggtacgat gccaagaaag tgacccaaag cagaccattc tctttgccgg
     1441 acggaacgcc ttagcggact tgttgaccta taccgttcca atgcccgttg gtggcgatgc
     1501 ggacctacgt gccttccctc catctgtcac ttgacccgat tcagacgtga tgagtgcgct
     1561 cgcatagggc aacaataaac aagagttaat gttcatgaga caacgcgcaa gatgtgctct
     1621 cgtacctaag aggtgaggct gccgagtgcg agatttaagg tggcgttctc atcactcggc
     1681 gacaggggat gccgcgcgtc ggaagacggc cccttctgtt ttgaaaacca tgctttgcag
     1741 caaatatccg agtgggcgaa tgtcgtttga gaacgcattt gacgatgcta ccgttcggtt
     1801 agatgcaagc tgagcgttcg gaagctgcga tgacgctttg atgggcatcg gcgagtaggc
     1861 tgggggatcg atccgtgacg aggttccgta tcaaggcaca tgcgcgtgcg cgcgctgctg
     1921 cgcacatcga acacagctac aaaatcctct ccgcggcact gactgcactt ttgatgtcga
     1981 cccgcatcgc gtcggcgcaa aattttgatt ccttcatgga tcaatatttc ggctacggcg
     2041 tgtttggcgc cctcgtgttc tggtggtggg tgcgacgagg gaaaaacaaa cgagtcgcgc
     2101 gcgaccaaca acaggcgtca gaacaaacgg aggaatttcg ccgcaatttt cctgagagga
     2161 acagtcgcga agcactggct gcgctcggcg ctacaaaggg cgaacgttcg caacgtattg
     2221 aagatatctt caatcggaaa agcgaaagcc tgacgtttga gatgcaagat atgaatccgc
     2281 gccaggaaac cggtgtcgtc gtgatcaccg ggaccttggt acgggcaatc gcagcaatgg
     2341 caccgcgcga acgtgaatcc ctgcgtgccg cctaccatct tttcaacatc aacgaatttg
     2401 accccaaggg cttttgccat gtgcgactgg cgctggtttt cggtgacgga aaagcacagc
     2461 acgcaaatgt aaatttctat gcgggtgaca atttaaaaca gaatcagaac gttcacaccg
     2521 tcgccgatgc gatcaaatat tggacgggga tgaatgccga gggtgccgta tccgcggttc
     2581 ttcacaggtt gcaggcggtc gccaccaaga tgccgcatga tccgacagta aagcagctaa
     2641 ccgggcgcct attgggcggc ggggatcagg cgctgtcgtc cacctgcgaa tttgcgcagg
     2701 cccaaggtgg aaaagaaggc gccggactga tactcggcat tgacgaagag accgggcaac
     2761 aggtggtctt cgcaggcgaa ggttccgtta ttacggtggc gccgccgggg tcgggtaaga
     2821 cgcaatgtca tgtctttcct accctgttgt cctggaaagg tccggccgtc gtattggacg
     2881 tcaaaggcga aatctacgcg gccaccagta agtggcggag tgagaacgtc ggccctgtct
     2941 ataagttctc accgctcgct cctgccgcaa gccacggcta caacccgctg acccccattc
     3001 gctccgatcc tgattatctc tgggaagact cgcggtttct ggccgatatg atgctggtgc
     3061 cgacgggcgg aaaagaccca ttttgggaga atcgcgctcg cgatgtgttg acggccgcca
     3121 tcgcgcgggt ttgcctcaac ccagacgttt ccaaacgatc cctcggtgat gtgctcgacg
     3181 tctttcacgg cgtcggctgg gatcagttcg tccgcgcgct tcagatggcg gtggatatac
     3241 gttcgatgac gcgggcagga agttctcttg gtgagatgga gcgcaagact cgcgacggcg
     3301 ttctgcaaac gggcctctcg agcctcagcg catgggacgg ggatcgtatt gaacgcgcga
     3361 cccgcaaatc ggattggagt ccgctcgacc tcagaaacgc caagagcccg acgatctaca
     3421 tttgtctcaa gcccaacgaa gtcgagagct acatctccgt tctgcgcgtt ttcatcgcgc
     3481 agcacattcg catgctaacc tcgtctttgc cgccaagagg cgcgccgtca attctgttcg
     3541 tcttggatga gcttccccgg ctcaggcaga tgccgccagt cgaggaagcc cttgaaatcg
     3601 gacgccaata cgggattaaa ctctggatgt tcacacaaag tctcggccaa cttgaaaatg
     3661 cctatccgaa tgcggaaggg atggtcggta actgcgctct gcgcatgtac atgaacccga
     3721 gccttcatga cgagacggcg cagaagattt ccgatgacat cggctttcaa gaaggtgttc
     3781 tcgacaatgc acgccagaaa atcgttgaag cccccgtctt ggcggggccg gactacaagg
     3841 attatgtgat cgttatggca tcgggcttca aaccgtttcg cgtgcgcaag agcttcgcgt
     3901 atcaggatgc gatgctcaaa tcacggatgg gaagcctcta gagctgcatc attcactggt
     3961 cctttcatgc tggaagcgtt catgcctgaa agcgattaca tcgatcggca agcagcgcgc
     4021 ctgcatcaag aaagacgcag cgccgaggct gcgctcgatc gtgttgatga cgcgcggcaa
     4081 tcgctgaacg agcagccgtc gagcgtcgag gtggctaaca gacaggcgga attggagagc
     4141 gagcaaaagc tggctgaagc cgaactcaat cgtatcgaag cggatttacg aaagatcgaa
     4201 gccctagagc cggaagatcg cgcgaaagaa gctaccctgg aaaccgacgc gaaactcaaa
     4261 ggtcaaatcg aaaaagccga tgcgctcaaa atcgaggccg ccgaggagga tcgtcgaaag
     4321 gaagaattct tcgcaaaaga gcagcgcgaa aagcaggaac atttcgaaga ccagacccgg
     4381 atgaaagaag gtctcgaaag aacggagcgc gaagggattg aagcaataga acaacaaacc
     4441 aaagacgcct ctgaaaaaag cgcggaggaa aacgtgatct cggagatcgc gtcggacctc
     4501 gcctctgatg gtctaaaaat cgcagacact ttgttggaaa tcgccgaatc cgcgaaattg
     4561 gagttggaaa aaagcgcgat ggcggaacgc cacaaggagg aactagagca actgcggcgc
     4621 gaccacgagc aggcccaaac agaactggtc gcgaggttgc aacaacgcca agccgacaat
     4681 agtgagattg accgggcatg ccaggccctc gtcaaggcgg ctgaaaggat tttggcagaa
     4741 ctactccagc ggcaggaaga agaacgccaa cggttggcgg aacagcgcgc ccgcgaagcg
     4801 cagcaaaggg cacagcgcgc cgcgagcgac ggattatctt tataagtgtt gcgcaagccg
     4861 cgcgcgattt gggtgtgcac agcaccgtgt tgcgcagctg ggtgaaggat tttgcggccg
     4921 atccgagtca ggcctttccc ggccatggtc agggccagca ctacgtgtct gatctcggcc
     4981 gatcgcaagc gttaccggta aagccgtcaa tcgggccact tgtctagcca cgcgcgtacc
     5041 cccgtttttt acccctgttt acagcttgga tactgctgta cgggatcgaa cggcgccaga
     5101 caaaatatga attttattag taaaatcagt gacttgtcat agtattccca tacgcggcca
     5161 aatagccaca aacgaatgga ggctggccat gggggcgcca atccatcaca gcggctttcc
     5221 ggtgagctcg ggatatttgt ggccgaagga gcgccgttcg ctcgcttccg gatgaagccc
     5281 tgcgaggaac gcccacgcat ctttcgtcca gcgcgcgtcg gcgagagcat gatggcgagc
     5341 gctcatttgg tggggcaatt cggggtcgcc cagctcgatg gcccattgtt tgatgtcgag
     5401 gcagagctgc ggaaatgaaa agggcagctc ggtcatgccg ccgaacagcc agctgaacac
     5461 cacccaatca taggcgggaa aatatcccca gaacaccgga tgcttgtcct catcgatgaa
     5521 ccggcgaacg gcataaccga tttcctcgcg gcttattccg atcccttcga gctgtggtcg
     5581 cacactattg agaatccatt gattggcctt ggtccaatcg acttcgctgc tttcggcata
     5641 aaattcgcgg ccgtcttcgg ccacgaggcc gatactaatc aggtcgatgg tatagggctg
     5701 cgcaataaac tcagtatcga tccaatactt cacaagacgg cccttcgttg gtggagcaat
     5761 tcgtcaggcg agtttcgcct gggcctcgcg gcaggtctac ggccagccac gccgtggatc
     5821 atcggttcaa ggctgggatc gatttcacat cgggcggcgg gggcaggctc gcgatgtagg
     5881 cgtggatgtt ggcgacatcg gaatcgggca tcgccgattg cgcgtagggc ggcatctcgt
     5941 tcacgggatg gcgcagttga ttgagaaacg cctcatagcc gatccccatc tgcgccaggc
     6001 gcaacccgcc ggtgcgtcct cccccgccct ggcccaccgt gccgtggcaa tagctgcagc
     6061 cattcgccaa atagagcgcg cggcctttga ccggatcggg tttgccggca tcggcggcca
     6121 gcgcgccgtc cgtcagtgcg ccggccgcca gcgacatcgc catcgtgaac actgcgaaca
     6181 cgcgtgaagc catcatcgtc cggtcctcag cgtccttgcg tcacgacatc gacgagaacg
     6241 ggttccccgc ccttgacgat cgcctttgcg cgcgtcagtg ccggttccaa atccttgggg
     6301 tcggtgatcg ggcctatggc cgcgatgccc atgccttggg cgatcttggc gtaatcgata
     6361 tagggatcgc gcagcgtagt gccgatatgg gcgcggtcga ggccgcgcat atggcgattg
     6421 gccatgcgct gcacatgcat cagttcctga tgatagccgc gattgttgtg catcacgaac
     6481 atcagcggaa ttttgtgatg cgcggcggtc cacaagacgc cgatggtcat caagagatcg
     6541 ccatcgcatt ggatcgccac cgtgaaccgc ccatgtttgc gattggcaag tgcggcgccc
     6601 accgatgccg acgcgcaata gccgatgccc atgccgccgc catcgccaat gaaatgataa
     6661 ggcttgtcca tggtccacag acgctgcggc cagtaggaga gcgaggcggt tggcgaagcc
     6721 atggaccaat cctcgtcctt gatctgattc cagacttcgg cgcaaagacg tcccgtggag
     6781 acgggactcg cgtcccagcc ataggtggca tcgacgcggg cgcgttcgaa atcggctttg
     6841 tgcatttcgg caaatttctt gccgcgcgtc tcaaacgcgg atttcttggt cccatcgata
     6901 tgggtccttg ccgcctcgat cagcatcggc atggtggcct cgccgtcgcc ggtaacggcc
     6961 atgtcgacat cggcgaagcg ttggaaatcc tgatagttgg atttggtgta gaggtcgccg
     7021 cttccaaggc tgatcagctt ggctccgggc ttggtgatcg gcttcgaggt gcgatgaagc
     7081 tggtcccggt aggaattgat ggtaccccaa taatcggtca tctcgagccc aagcacgacg
     7141 tcggcctggg aaagatgggc gcgcgcgcgg ctgctctggt tcagcggatg acgcgaaggc
     7201 agattgagcc gtccgctggt gtcgatcacg gggcagttca gcgcttcggc caattcgacc
     7261 agcatcttgc ttccttgcga cgtgcgcgca aagcggtcgg cgatgagaac gggattttcc
     7321 gctgttgcga gaagccgcgc ggtttcggcc acggcccccg attcgccctg tggcggcgaa
     7381 atcttgggaa gctttggaat gcgcaagtcc aaaccgtcgg gaatcgggtc ttcctgcagc
     7441 gcgccatcgg cgaccagaag cacgggcagg gtcgggggtg tcatcgcgat tttgtaggcg
     7501 cgcacgctcg attcggcgaa gtgctgcagc gacgcgggat tgtcgtccca cttcgtgaaa
     7561 tcacgcacca ccgcggccgg gtcctgtgcg ctatggttcc attcgatgcc gggacggcgc
     7621 aactgcgcgt ccatgatatt gccgatcacg acatagaccg gcacgcggtc gcaccaggca
     7681 ttgtagagcg ccatggtggc atgatgcagc ccgacggtgc cgtgggcgaa gacgcaaagc
     7741 ggcttgccct cgatcttgta atagccatgg cccatcgcga ccgagatttc ctcgtggctg
     7801 caggtgagga attcgggatt cgcattaccc ccgtagttga cgagcgactc gtgcaggccg
     7861 cggaaactcg aagcgggatt ggccgccata tattcgaagc cgaggctctt gatgcagtcg
     7921 accatgaaat cgctgccgct cttgccttcc gtcagcactt cgagttcggg cggaacggct
     7981 gtttcggcgg ctttgttcgg cagcggaacc gggttgttcg cagggccggg ccgcacggct
     8041 ggctgcgcca ctgcattcag tggcgtagca aaagccgccg cgccggcgaa ccccgcgcct
     8101 ttcaggaatt tgcggcgctt ggcgcttgga agatcggctt ttccggtcat caaacagccc
     8161 cgatacctat aggtccgaac gaaccttaaa cgccggttcg aacgctgtcg atcatcgagg
     8221 cgcgattccc aaaatggacc tcgtgccaat tgcgctgtac gcttaaagaa aaacaggtag
     8281 ggggcatcaa atggtggcga tcatgacccg gataatttcg gcggctgtgc tcgttcttgg
     8341 gttggggact atgaccctcg cgtttgccca aacgccactc gaacaaagcg ccgaacaccg
     8401 cttccagctc gactttcatg tctcagatac ggcacttcag aagatgctgc cgcaagggtg
     8461 ggctcccgcc gtcgcggccg caggtccggc aaaagacgcc aatctgcgca tgatcttcat
     8521 cgaccgcatc ggtatcgtcg ggaccgatgg caagccgctg ggccgaggtg ccgcgcgcat
     8581 ggtctatctt gccgttccgg tgaaacagac agccggcgat acggccggcc agatgatcgt
     8641 cgctggattg acggacaatg ccgccgacgc gccgggggct ttcggcgtct atcaatatgc
     8701 ggcaaccgcg aagatgacgc gcagtgtgaa cgcgtccaat ggcaccgcga tcgcggagga
     8761 ggattgggat ttcgtcggca acggtggcga gcacatgcag gtgcatgtga aatatgaacg
     8821 tgcgcccgcg gccaaaggcg gcggggaggt gaaattcttc aatcccgccg atccttcgaa
     8881 ataccagatc ttcaagaccg aacagacaac ggacattacc cgcaacgcga cgaccaatcc
     8941 gccggaccgc gtcaaggaat tctcctatcg tgccggcggt ggacgcattg catcgctttt
     9001 cgatggtacg gaaaaggtgc tgagctggga ttcgcagccc tggtacaacc gcagcgtgat
     9061 cgcaccgtga aaatcgcacc cacaagaatg ggggatgcaa agatgcgcca tttgagattg
     9121 ccgcatgttg gattggccct gggcctcgtc gttgggactt cggtcgcagc acaagagaca
     9181 gcgaaaccgg gcctgcccga tacgatcccc tgcccggaag cggttgcgca gatcgcgacg
     9241 tgctattccg cgaaactgga cagcggcgct tatgtgctcg ccgcgatgcc gaagcattgg
     9301 aacggcaatc tcatcgtctt tgcccatggc gggccatcgc tcgtgcccgc gactgcgaca
     9361 agcagcaaag gcgatctttc caaatattct gtcgaggtcg aacggggctt tggatggata
     9421 gcctcgagct accgccgcga gggctatggg atacagatgg ctgtgtccga taccgaggac
     9481 gcgcggaaat tcttcatcgc gcgcatcgcc aagcccaagc gcaccatcat tcacggcgcg
     9541 tcctatggcg ggctggtggc ggcaaagctg gtggagaccg tcgcgaagga tgccgatggc
     9601 cgccccctct atgacggcgc gttcctgaac agcggactcg tttccggttc tgcggtcggg
     9661 tatgaattcc gtgccgactt acgcgtggtt taccaatatt attgcaaaaa tctgccgcgt
     9721 gcgaacgagc cgcaatatcc attgtggctg gggttgcccg ccgattccaa gctgacgctg
     9781 aaggagatgg aatcgcgcgt cgacgagtgc acgggtgttt ccaaacccgt caccgaacgc
     9841 acagaacggc agaagcagaa tcttgccaac atcatcggcg tcatgggctt ttccgaccgg
     9901 ctgctcacgc gccacatgca gtcggcgacg tacatattcc gcgacattgc cacgcgcata
     9961 acccaaggca aaagcgcgtt cacgaatatc ggcatcgtct atcgtggctc ttccgacgac
    10021 gcggcgctga atcacgagat cgttcgcttc aatgcggcgc cggaggcgcg cgcggcactg
    10081 agggcagacg gtcagcctac gggcgcgttg ccgattccaa tggtcgccat tcattccatc
    10141 aacgacccgc aggtggcggt ggaggtggaa tcggcctatc gcgatgcggt gcagggtgcg
    10201 ggcaacgctg agcgtctggt gcaggcctat acggatgaga atcagcacac cggacagagt
    10261 gcgccggaaa tcgcggcggc gctggaaatg ctgatgcaat ggatcgagat ggactacaaa
    10321 cccgatgcgt ccatcatcgc ggaggcttgt gcgcaattgc gcagcggctt tgacggcccc
    10381 tgccgctacc atccggaatt ccagcccaag gcctatggca caaaatttta cacgcgcgac
    10441 gtggtggcgc gctaaaccgt cttcacggcg accttgcgcg cacccatctt gcggcccatc
    10501 accagcacga tcaggcccga gatcatcatc gcggctccca tggcggccat ggctgagcta
    10561 tatccgccgc tcgattcttt caacacgccg acgccgtagg gcccgatgaa gcgccccagg
    10621 cttccgatcg tgttgacgaa cccgactccg cctgccgcgg ccgtaccggc gaggaatgtc
    10681 gagggcaggg cgaagaacgg tccgttgtag gcgagcgtcc ccaccaatgc gaccgcaaga
    10741 gacagcagga caagcatgtt cgattgcacg agggctgcga acatcaaccc gaacgcgcca
    10801 agcagcaacg gaaccgccac atgccagatg cgttcctctg tcgcgtcgct gtggcgcgcc
    10861 caaaggatca tcgcgaccat cgaagcgatg aagcaaagcg caacgacgaa acccgtcgcg
    10921 aaattcgaga aacccatgtc cttgacgatc tggggcagcc acaattgaac cccatacagc
    10981 ccgatctggt tgcccaacag cacaagtccg agtgcgatga ctcgcgggtc gcgcaaagcg
    11041 ggccagaaat cgcgatgctc gctgctgcct tcgtcttttt tgagccgcgc tgtgacgatc
    11101 cgtttttctt cgtccgtcag aaaacgcgct tctgccggcc ggtccggcag aagtttcaga
    11161 gaaagaaatg ccagcgcggt cgcaggtagc ccctccagca ggaacatcca ttgccatcca
    11221 tgcaggcctg cgatcccatc caggcccagg atgctggtcg aaagcggacc gccgatgatg
    11281 ttggcgagcg gaattgccgc catgaaaacc ccgacgaatc ggctgcgcca ggactgcgga
    11341 aaccaatagg tcatgtagag cagcatgccg ggaaagaatc ccgcctcggc gatgcccagc
    11401 aacagacgca gcgtgtaata gctgcgcggt ccctgcatga aggcgttgca ggcggataac
    11461 agaccccaga cggccaggat caggaatatc cagcggcgtg cgccaagctt ctccagcatc
    11521 aaattggccg gcacttggaa gatcatgtaa ccgacgaaga atatgctcgc gccgaacccg
    11581 aacacagccg gggagagacc aaggtccttg ttcatggtca gtgccgcgaa accgacattc
    11641 actcggtcga tgtaattcac cacatagagc agcatgacga aggggagcag gcggcgtgca
    11701 atcctcgcga aaatctggtc ttcggtcata tgtcgtcctg ccctgttgcc atttgcacga
    11761 tgagccgctc tgtgcgttcc ggcagctctt tgaccacgct agcataatct tgaagcttcc
    11821 cggaacgcgg acttgtcatc ggacgaaaat ccacgccaaa ttgcataagc gctccgcgac
    11881 aggcacaggc tgcgttcgcc caaggttgga atcgtgaaga aagtcgtcgt tacaggcatg
    11941 gggtgcgtct cgggtttggg gccggatagg gcatctgctt ggaaaacgct catggccggc
    12001 gaaggaggga ttcgccccgt cgtgaagttc gcccagggcc agcaagagat gcgcatcgaa
    12061 gggcccggag cagctgtccc gccagacgct gcaaaaaggc tgaatgcgca ttttaccgaa
    12121 aagcagcttg caggcgtcga tccattttcc acatttgcgg cggctgcgac acttgaagcc
    12181 ctcgaggatg cgggactttg gggaacttcc gccagcctgg gcgaagccgc aatcatttat
    12241 gggagcgcca gcggcggcaa tcttgcggtt gaagccggct atgtgcggat gtttctgcag
    12301 ggaatgccca atgtgcatcc gatgacgatc ccgcgctgca tgagcagcgc atcggtcgcg
    12361 catctgtcga tgctgttcgg catccatggc cattcgctcg ccgtgtcgag tgcctgttcg
    12421 tcctcggccc atgcgatcgg tgaagccatg catctgatcc gcgcggggcg cgcatccatc
    12481 gtggtcacgg gcgggagcga ggctagtctg acattcggat cgcttcacag ttggaaggct
    12541 ttgcaggcga tggcgcccga tgcttgcagg ccattctcca tggaccgcaa aggcatggtg
    12601 ctgggcgagg gcgccgcaac cctgatcctg gaggaagaag gtcacgcgct ggcccgcggt
    12661 gcgcacattc atgccgtagt cgcgggctca ggctcgacct ccgacgccag ccacatcacc
    12721 cagccggatg ccgaaagtgc cgccgcggcg attcgagccg cccacagaga tgcggactta
    12781 agcggggacg cccctatttt gatcagcgcg catggaacag gaacgcggct gaacgatcgg
    12841 tcggaggcct ctgcgctgcg cctggcgtac ccgtccagtc ttgaccgcca tcgcgtgatc
    12901 gcgacgaagt ccgcgcacgg ccatatgctg ggggcgaccg gcgcaatgga atttctgctc
    12961 gcgatcgagg ccctgaatgc gcaaatcgcg ccgcccatcc tcggctacct cgggcccgat
    13021 ccggaatgcg atttgccttt ggttctggcg cccgagaaaa tcgcatgcga agccaccgtc
    13081 tcggcatcat tcgcatttgg agggctcaat tgtgtcctca ttgccaaaaa gaaatgagcc
    13141 ccctgccaat tgatggatag gggtttgtat ggtgaagtaa accatggatc tcgaacaaga
    13201 attgcgcgcc gtattgaaga cgaaactccc ccctggcgct gaaatcgata tgaataccaa
    13261 actcgccgat gccggcctcg acagtctcga tgtcgtcgag atcgcgttcg atgttgaaga
    13321 caagttccgt atccaactgc ccccgctggc gggccagatc acggacgtca catttttgga
    13381 cttgtaccgg ctggtcgaag agaagatcgc cgcgaaagcg gcagagacaa tttaggctgg
    13441 tcgcgtctcg ttcaggcgct gttcacgtcc aaagccgttt cctttcattc gcgggtcatg
    13501 tgagggcgta tttgaccgaa gcgcatatca accgaatcgc aacggccgtc ccgccctatg
    13561 atgttcacca ggcgttcgtg caattcggcc gttcgctgtt gacgcacgac gaacgccgct
    13621 cggctctgtt cgaacgcatg gtggatcgcg cggagatcga tcatcgctat tcttatcttt
    13681 cgcccaatga acgcgcggac cagggtggca tgtcggcaat cgatttttac acgcgcaaga
    13741 gcttccccag caccggcgcg cggatgaaat tgttcgagga tcaggcgccc atgctcgccg
    13801 aaatcgcgat aggacggctt aatcttcggc ctgaggaacg cgcgcgcctt acgcatatcc
    13861 tgatttcctg ctgcacggga ttttccgcac ccggtctcga tctggaaatc atgaaacgat
    13921 gcggccttcc cgggtccatg gagcgcagca tcatcggatt catgggatgc tatgccgcga
    13981 tcaatacgct caagcttgcg cgtcatatcg tgcgctccga gccgcatgcc cgcgtgcttg
    14041 tgatcaattt ggagctctgc agcctgcatc tgaaagagac atccaatctc gatgaaatcc
    14101 tctcatttct gatcttcggc gatggctgcg cggcttccat cgtgacggct gagccggtgg
    14161 gtcttgcgct cgacagcttt cgtgccgtgc tggttcccga tacgcacgat ctgatcacat
    14221 ggcatatcgg cgataccggt tttgacatga ttctgtcagg gcaggtgcct ggagcgatcc
    14281 tggaagcgct gcgcaaaggt gccggtgaaa tcctcgatgg tgcgccggtc agtgcctttg
    14341 atttatgggc tgtgcatcct ggcggccgtt cggtcctgga tgcggtcgaa cgcgctttcg
    14401 atcttcgcag caacacactt tccgcgtccc ggagcatttt gcggcgcttc ggcaacatgt
    14461 cgtccgcgac ggtgatgttc gtgctggaga ccatgcttcg cgcaagtgcg cagacccgtg
    14521 gctgcgcgat gtcgttcggg ccggggttga tcgcggagac catgctgttt cacggcacgg
    14581 gctagcggtt tctgcggacg attttccatg gctctgcttc gcacaatgga acccgaatgg
    14641 ctggatgaaa taccggccga tgatttccgt gcgatccgtt cgcgccgcga cctgaagcgg
    14701 atcaacgcct tcatgcttca aaccgcgatt ctggcgcggc tgttgacacg acatcaaacc
    14761 acgccgccgc ggagaatcat cgagcttggc tcgggcgatg ggaccttcat gctgggcgtt
    14821 gcgcggcgcc tcgcttcggt ttggccggag gtccacgtcg ttttcctcga tcggcaaaat
    14881 atcgtcagcg atgaaacacg cgagaatttc cgggcgctta attggcagac cgaatcggtg
    14941 agcgccgatg tgtttgattt tctcagtgcc cagaagccgg ggagcgcgga tatcgtcacg
    15001 gccaatctgt tcctgcatca tttttccgaa caggccttga gcaggctctt cgcctttgcc
    15061 gcgcaattgg cgccgttgtt tgtcgcctgc gagccgcgcc gttcgtcttc cgctctgatc
    15121 gccagccata tggtcatcgc gctgggctgc aacgacgtct cgcgtcatga tgcggtggta
    15181 agcgtgcgcg ctggcttttc gggtcaggag ctttcgaagc tctggccaca ggacaggggc
    15241 tggaacctgc aggagcaacc cgcgctgccc ttcacccatt gcttcgcagc ccgccggatt
    15301 gagcgcgccg gacatgccga tgtatgacgc gctcatcatc ggtgccggcc cggcgggatc
    15361 gacggccgcg cggctcttgg caaaggccgg ctggtcggtc gcattggtcg agaaaagcga
    15421 atttccccgg cgcaaggttt gtggggagtt catttcggcg accagtctgc cgcttcttta
    15481 cgatcctcat gtgcgcgaga gctttctttc tgaagcgggg cccgaagtgc gccgcgtcgg
    15541 attgtttgcg gccgatgcga tgttgacgtc cgcgatgccg gcggccgatg cgtcgcgaac
    15601 cggctggggc cgggctttgg gcagggaccg tctcgatctg ttgctggtcc agggggccgt
    15661 ggctgccggc gccgaggtct tccagccctg gaaagtcgtc gtattacagc gcggcccggg
    15721 agaatttacc tgcaccatcg cggcgggcga cacgcgtcag gatctctgcg cacgcatcgt
    15781 gattgcggcc aacggatcgt gggagaaaag ttcctgggag acgacacgcc cacggaaccg
    15841 tgcagcctcg gagctgcttg gcttcaaagc gcatttcagc gatagcgcac tgcccagcga
    15901 tgtgatgccg ctgctggtgt tcccgggcgg ctatggcggt atggtgcaca cggacggcgg
    15961 ccgtgtcagt ctttcctgct gcgtacgccg ggacacgctg gatgcttgcc gccgcgcgca
    16021 ccggggcctg ccggcaggtg acgcggttct ccagcacatt atgcaccatt gcgccggagt
    16081 ggcggatgcg ctgctgcgtg cccgcatcga tggcggctgg ctttccgccg gtccaatccg
    16141 gccgggcatt cgcccgcgtt acgcaggcgg aattttcttc gtgggcaaca gcgcgggcga
    16201 ggcacatccg gtcatcgcgg aaggcatcag tatggccatg caatcggcgt ggctcctctg
    16261 ccagcgtctc atggctgcag aggatcgaac ggcgatttcc gcttccacgt ccgaaattgg
    16321 acggctctat gcaaaggact ggcatagggc ttttgcggga cggattcgcg ccgccgcgat
    16381 gttcgcacat cttgcgatgt ggccgagtgc ggcgcgtgct ttgccggtcg taaagcggtt
    16441 cccggaaatt ttgacttggg gcgcgcggtt gagcggaaag gccacccagc tcgtgccatg
    16501 atgcggccgt gatgcgggca ggcttgctgt gcaggcggcc tgccgaaatg gagattttcg
    16561 cgtggcggcg aacggcactt gggaattctg gatcgaccgc ggggggacct ttactgacat
    16621 cgttggccgt acgcccgatg gggctttggt cgtgcataag ctcctttccg aaaatcccga
    16681 acaatatgac gatgcggcca ccgccggcat tcgcgcactg atgatgcgtc atggcgaggc
    16741 cccgatcgca gcggtgaaaa tgggcaccac catcgccacc aacgcgctgt tggagcgcaa
    16801 aggcgaaccc accgttctcg ccataaccgc gggccacagg gacgcgctga ggattggcta
    16861 tcaaagccgc cccaatctct ttgcgcgcca tatcgtgctg ccggaaccgc tctatacgca
    16921 aattatcgag attgacgaac gcgtgacggc ggagggtgaa atcctcaagc cgctcgatga
    16981 agctgccgcg cggtgcgcct ttgaagcagt cttcgtggat ggctatcgtg ccatcgccat
    17041 cgttttgatg catggctatc gccacactgc ccatgagaag cggcttgaat caatcgcgcg
    17101 cgcagtcggt tttacgcaga tttccgtcag ccacgaggtc gcggcactca tcaaacttgt
    17161 gccacgtggc gataccagtg tgacggatgc ctatctgtcc cccttgttgt tgcgctatgt
    17221 cgaccggata cgtggccgct tggatgagaa aaccagactc tttttcatgc aatcatcggg
    17281 tggcctggcc gaggcaaacg cgtttcgcgg caaggatgcg gtgttgtccg gccccgccgg
    17341 aggcgtcatc ggactggcga aagtggtcgc tgccgcgggc ttcgccaagg cgattggctt
    17401 cgacatgggc ggaacatcga ccgacgtgtc ccattacgcc gggatttacg aacactcgcg
    17461 cgggaacgcg attgcaggcg tacgcctgca cgtccccatg ttggacattc gcaccgtcgc
    17521 tgcgggcggc ggctccattt gccgcttcga cggactgcgc cttcgtgtcg gccccgattc
    17581 ggccagtgcc attccagggc ccgcctgcta tggccgcgct gggcctttga cggtgacgga
    17641 ctgcaatctc ctgctcggca aattgcagcc ggagttcttt ccgcgcgttt tcgggtgcac
    17701 cggcgatggc cctttggaac gcggcgttgt ggcgcggaaa ttcgccgagc ttgcggtaga
    17761 gatcacggcg tcaaccggaa aatctatcac gccacaggag atcgctgaag gtttcctgcg
    17821 cattgcggtg gccaacatgg ccaaagccat caagcagatt tccatccaac gcggccatga
    17881 tctggccgag tacacgcttg tcgctttcgg tggcgcggcc ggtcagcacg cctgccttgt
    17941 cgccgatgcg cttggcatga agcgcgtgat gatccacccg cttgcgggcg tgctgtccgc
    18001 ctatgggatt ggacgtgccg agctgaggac catccgcgaa cagacgatga atgttcgcct
    18061 tgaggcggca gtcgatcgtc tgggggccgt agtcgaagtg cttctacaag acgcacgtga
    18121 atcattggtc aggcagggcg cggcgccaac ggctatccag ggcttcgcac gggtggaatt
    18181 gcgctaccag ggtgtggatt ccacgctttc cgttccgctt caggggatcg cacagatgcg
    18241 cgcggcgttc gaaaatgagc atcgcaggcg tttcggcttc gccagcgcgg gcaagagcct
    18301 cattgtcgaa tccgtttcgg tagaggccgt ggaattcgcc acgacgagcg aagagccggc
    18361 ctctcgtcct caaaccgtcc tcgcttcgcc attggtacag gttcgtgcgt gcctgggcgg
    18421 cgaggagcgg gagacacaag tgtatgaccg tgacgccatg ctggctggag tggagatcgc
    18481 gggtcccgcg atcgtcaggg aaacgaccgc aacgaccctc atcgaaccgg gatggcgcgc
    18541 gcgcgtcgat ccgcttcgaa atcttatcct ggagcgcgcc cacccctcgc cggcgtacag
    18601 caccatcggc acggcggccg atcccgtctc gctggaactc ttcaacaatc ttttcatggc
    18661 ggttgccgag gaaatgggcc tttcgctgca gaacaccgca tcttcggtga acatcaagga
    18721 gcggctcgat ttttcctgcg ccctattcgc ccccgatggc tcgctgatcg cgaatgcgcc
    18781 gcatatcccc gtccatctcg gttcgatgag cgattcggtg cgcaccatca tcgcggcgcg
    18841 cggcttcgtg agcgacgggt atccgcgcga tggccgggga atcaaagacg gcgatgtcta
    18901 tgtgctgaac gcgccctatc ggggcggctc gcatctgccg gatgttaccg tcatcatggc
    18961 aatcttcgat gatggttggc gcatcgcggg ttttgtcgcc gcgcgcggcc atcacgcgga
    19021 tatcggcggg ataaatccta gctccatgcc gcccttcagc cgcaccgtgg aggaggaggg
    19081 ggtccttttg gacaatgtcc tgctcgtcga tgagggtcgt ttccgcgagg aggaaatgcg
    19141 tgcactgctc gcatcgggcc gctatcccgc gcgcgatccc gattccaata tcgccgacct
    19201 gaaggcccag gtcgcggctt gtgtcaaagg tgccgctgaa cttcgcgcgc tgatcgggca
    19261 ttacggccgc gatgtcgtgc acgcttacat ggttcatgtg caggacaatg cggccgaatg
    19321 cgtgcgacgc gtcatcgata cgcttgcgaa cgggtctttc tcctgcgaga tggacaatgg
    19381 cgcggttatt cgcgtggcaa tcatcgtcga tcgtgcaagc cgccatgccc ggatcgattt
    19441 caccggaacg agcgcgcagc gcgacgacaa tttcaacgcc cccctgacgg tctgccgcgc
    19501 tgccgtgctt tacgtcttcc gcacgcttgt cacagatgac attcccctca atgagggctg
    19561 tctcaggcca ctcgaactcg tcgtaccgga aggctgtttg ctcaatccca attatcctgc
    19621 cgccgtggtt gccggcaatg tggagaccag tcaggccata acggatacgc tttatggcgc
    19681 gctcggcatt caggccgcgg cgcagggaac gatgaacaac ttcaccttcg gagacggtgc
    19741 gcggcagtat tacgaaacca tctgcggcgg ctcgggagcc ggtcccgatc atcacggcac
    19801 cagcgcggtc cacacgcata tgacgaacac gaggctcacc gaccctgaaa tcctggagac
    19861 gcgattccct gtgctggttg aggaattcgc gattcgcaaa ggctcgggtg gcggtggccg
    19921 ctttcatggc ggagacggcg tggtgcgcaa gatcattttt cgcgaaccta tgacggcgtc
    19981 gattctatcc aaccgccgcc gcgttgcacc tttcggtctc catggcggtg cgcccgctct
    20041 ctccgggcgc aatcgggttt tgcgcgcgga cggcacggtt gaggattttg gcgcctgtgc
    20101 aacgactgcg atgcagcccg gcgacgtgtt catcatcgaa acaccgggtg gcggtggttt
    20161 cggaccgcgc gcctccacgg aatgagggtg gaattgcgcc ccggatccgt tcgtgaagaa
    20221 tttccgcggc tcgatgcatg cacagcctcg cgcttggccg ccatcgcgct cggtcatgtg
    20281 acgcgcgaat atcccaatcg gctcgaccat atgctcgaag ggcctgccga tttggaatcg
    20341 ccgcaggcgc tgcatccggt tttctacggc agtttcgatt ggcactcttg cgtgcattcc
    20401 tattggctgc tggcgactct gatccggctc tttcctgaga tgcccgaagc aagcgccatt
    20461 cgcgcgctct ttaacaacac attcacgaag aagaacattg cgggggaatg cacctatctg
    20521 gagcgcaaat cggcggcgct gttcgaacga ccctatggct gggcatggct tctgatgctt
    20581 caggcggagc tgctgcgcca tgggggcatc gagggtcggc ggtgggcatc ggcgttacga
    20641 ccgctttccg cgatgttcgt cgcgcggttc ctcgcgcacc ttgccaaagc gacctatccc
    20701 catcgcgccg gaacccacgg caatagtgct ttcgcgctgg cgctcgcgct ggaatacgcc
    20761 cggacggcaa gggacaaggg gcttgcgatg ctgatccgga aaaccgcccg gcgcttttat
    20821 gcgaaggacg ccgattgtca ggcatgggag ccaagcggcg aggatttcct gtcgcccgcg
    20881 ctgatggaag tctactgcat gagtcgcgtg ttgccggcgc agcgcttccg cgcctggctt
    20941 tcccgtttcc tgccgcgatt ggcggcgcaa aacccggcga gcctgttttc accggcacgg
    21001 gtaagcgacc ggaccgatgg ccggatcgtg catctggacg gtttgaattt gagccgcgcc
    21061 tggtgctggc gcgcaatcgc cgggacattg ccggagaacg accgcgccgg ccccgtcgcg
    21121 gcccgtgccg cgcgacgcca tttcgcggcg gcggaaggtt ttctcaccgg ccattatatg
    21181 ggcgagcatt ggctggcgag tttcgcgctg ctcgcgcttc gttaaggacc catggctccc
    21241 gtgaacagct tctacaccat tggacattcc acgcgctcgc ttgacgcgtt tgcggatctc
    21301 ctggtttcct cgggcgtgaa ggagcttgtc gatgtccgca ccatcccgcg ctcgcggacc
    21361 aatccgcaat tcaacgcgga tgttttgccg gaatcgctgc gcccctttgg aatcgtctat
    21421 cggcatatcg ctcaattggg tggcttgcgc ggccggcgga aggatcgtcc gcaatccccc
    21481 aatacattct gggaaaatag tagcttccgc aactatgccg actatgccgg cacggaacca
    21541 ttccgcgagg ggctttcgaa cttgcgcgag atcggccacc ggcaaacctc tgccatcatg
    21601 tgcgccgaag ccgtatggtg gcgttgccac cgccggatca ttgcggacta tctgcttagc
    21661 ctaggcgaga ccgtgttcca tatcctcgga ccgaataaaa tcgagcccgc ccacctgacg
    21721 cccgccgcgc agcaggccgc cgacggaacg ttgctctatc ccaaatgacg cttcaggatc
    21781 ccatgatcgc gcggacaatg aaaaacagga tcgacggcgc gaccaggcag cctagaccag
    21841 tatggaatgt cgccgtcagc gcgccatagg gaacgaggcg aggatcggtc gccgcaagcc
    21901 ccccggtgac accgctgacc gttcccatca ggccgccgaa cgccatcgcc gaacgcggat
    21961 tgtccagtcc gatagaacgc gccacgaagg gcgtgcccgt catcaccagc acggccttga
    22021 agacgccggt ggcgatggag agcgccatta ccggcgagct tgcgcccaag gccgagcccg
    22081 tgaccggccc cacaatatag gtgatggcgc cggcaccgat ggttgtcatc gagaccgcat
    22141 ccgaataacc gaaagcgcgc gcgacgctgg tgccgacgat aaagggaagg atggtcccaa
    22201 gcgtgagtgc aatcgcgccc agcaatcctg cctttttggc ttcgttcaca tccacttcga
    22261 acgcggtcgc gacgatcgcg aaatcgcgga tcatcgcgcc gcccatgatg cccagacccg
    22321 cgaacattgg aatgtcggca agcccgttct tgccgcccgt cgcgatgccg cccgcatagg
    22381 ccaggaccag acccagtatg atcgcgatgg ccgagccgtg gatgcggccg aacgtcagat
    22441 atttcgaaac cgcataggac agccacatca caccgcctac ggcggcaaaa gcgacgatca
    22501 atgcgtgctc gtcggccaga cgtttcagga tgtcggtcat tccgcggcgc ccgacagcac
    22561 gcgcgcggca tgggcttctt ctgcgctggc cggaacggcg tcgcgcgggc gcgaccaccg
    22621 gctcaggagc gcggtcgccg ccagactgac aagcacgccc ccgcctcccg ccagcaacac
    22681 cacgggccca ccgcgcacgg cggcgaccac attttggatc gaagccatgg caaccacgat
    22741 ggggatatag agcgctgccc agaactcgat gccaagtttg aacccatggt tcagcttgcc
    22801 ccgttgcatc agccaggcgc gcgccgcgat caggagcagc atggcgatgc cgacgccgcc
    22861 gacattggtt ttggccccga gtgcgacacc gagcacatcg ccgagcaagg cgccggcaag
    22921 cgtgcagatc gccagcaatg caacaccgaa aatggtcatg ccaagcgtct cctcttcgat
    22981 gtccctctgc gcgcgcggga actgtcgtgc cagaaactac aggcaggctc aaaccctagt
    23041 caatttgcct gcgaaccatg ataggaggat ttacactgtg gaatggctga aatcatgggt
    23101 ctgaacggga tgcgttggga tgggttctga agtcgccgca agcgcgcttc cgctcgccgg
    23161 actgatcgtg gcggagttct gtaacgtcgc cgcggggcct ttctgcggca tgctgctcgc
    23221 cgatatgggc gccgacgtgc tcaagatcga accgcccggc ggcgatatgc tgcggcagtg
    23281 gccgccgcat accaacggct tcagcgaaaa tttcgcctcg ctcaatcgcg gcaaacgctc
    23341 catcgccctg gatttgaaaa atcccgccga caatgaaatc gcccttcgca tcatgggccg
    23401 cgccgatgtc gtcatcgaga acaaccgccc cggcgtcatg gaaagattgg gactcggctg
    23461 gctgcgcatc catgccgaga agccgaaact cgtctattgc tccatctccg cttacgggca
    23521 gtccgggccc cgcgccggcc agggcgggtt cgatgtcacg atgcaggcgg cttccggatt
    23581 gatgagcgtc accggcgaga cggaaggcgc gcctgtcaaa tgcggtgtgc cggtggccga
    23641 tttcaccgcc ggcctttacg gtgcgttcgc gatcgcggcc gcgatcgccc gcgtgcgcgc
    23701 aggcggcgaa ggcctataca ttgatgtccc gatgttgggg acgacacttg ccatcggcgc
    23761 gctccagacg agcgaatatt tcggcacggg ccgcgatccg caggctttgg gctcggcgca
    23821 tccgcgcaac gcgccgtatc aggcctatcg cgcgcgggac cgctatttcg tgattgctgc
    23881 gggaaacgac aaattatggg ccagcgtgtg tgacattgtt gaaaggccgg gactcgtgtc
    23941 cgatccgcgc ttcgccaccg tc
//
LOCUS       DASYEG010000119         2611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_181492, whole genome shotgun sequence.
ACCESSION   DASYEG010000119 DASYEG010000000
VERSION     DASYEG010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181492"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VGM72_05045"
     CDS             complement(<1..626)
                     /locus_tag="VGM72_05045"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY4264662.1"
                     /translation="MEAQPDRPAQQRVQGKRASIKEQNRRLILDAARRVFAESGYGGV
                     TVRDIIRATPLASGTFYNYFRSKEEIFQAMRDETALALRPVLRDARRDAGTSEAFVAE
                     TFRAFFTYVAQRRGTVAVIRRNDAAPLRVDTPEVLAGFEELKADVEAAIARGLFAPVD
                     AEYLAASMLGIACELAEILQWREIPEPDEIARFATALVLGGLAALPKPV"
     gene            complement(726..1433)
                     /locus_tag="VGM72_05050"
     CDS             complement(726..1433)
                     /locus_tag="VGM72_05050"
                     /inference="COORDINATES: protein motif:HMM:NF019180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043461 - proton-transporting ATP synthase
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP12 family protein"
                     /protein_id="HEY4264663.1"
                     /translation="MNARRFYREVSVEAVTDGGYSVLLDGKTIRTPTGAALALPAAAL
                     AEAIAEEWRGQADTIRPETMILTTLANTAIDLVRPNRQIAIEQILAFARSDLVCYRAQ
                     GPENLVRRQAAEWDPLLDWARTRYGATLVCGAGIVHVEQPPQTLEALEGAIAQYDDFE
                     LAGLHAAATLLGSAIVALALSDRHVSAEQALAAAQLDELYQAERWGQDVEMLNRSRKK
                     TAELTDITEYFLFLRVC"
     gene            complement(1430..2437)
                     /locus_tag="VGM72_05055"
     CDS             complement(1430..2437)
                     /locus_tag="VGM72_05055"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY4264664.1"
                     /translation="MSATTAVQEDEAGLRLDRWFKRHYPAIGHGPLEKLLRTGQVRID
                     GKRAHAGDRLEAGQAIRIPPQLQGQTGAQPNGPRAHSFSDADKDFMERLVIHEDPSVF
                     VLNKPSGIATQGGSGISRHIDGLLEALQGKKKQRPRLVHRLDRDTSGVLVVARTLPAA
                     AALAQSLRRRDARKIYWALTRGVPIPHQGVVRLALAKEAGFGKHGRDERMAPATEDSE
                     DAKSATTHYAVMGTAANQYAWVALRPVTGRTHQLRAHLAHLGTPIVGDFKYGGENAKG
                     LGELEDRLHLHARSIDMAHPEGGRLRATAPLSPHMVHAWRLFGFDPNSTADPFADSRK
                     L"
BASE COUNT          483 a          856 c          794 g          478 t
ORIGIN      
        1 accggtttag gaagggctgc gagcccgccc aagaccaagg cggtggcaaa acgtgcgatc
       61 tcatcgggtt ccggtatttc ccgccactgc aggatctcag ccagttcgca agcaatcccc
      121 agcatggatg ccgcgagata ttccgcatcc acgggtgcga aaagaccgcg cgcgatggcg
      181 gcctctacat cggccttcag ttcctcgaag ccggcaagca cctccggcgt atcaacacgc
      241 aagggcgccg catcgttacg cctgatcaca gccacagtcc cgcgcctttg cgccacatac
      301 gtgaagaagg cccggaaggt ttccgccacg aacgcctccg acgtccccgc gtcccgccgc
      361 gcgtcacgca ggaccggccg caacgcgagc gcggtttcgt cccgcatggc ctggaagatt
      421 tcctctttcg agcggaagta attgtagaag gtgcccgaag ccagcggcgt cgcccgtatg
      481 atgtcgcgca ccgtgacgcc gccataaccg ctctcggcga agacacggcg tgcggcatcg
      541 aggatcagac gacgattctg ctccttgatg gatgcgcgct tgccctggac acgctgttgc
      601 gccgggcgat cgggctgggc ttccaaaggc ggtccttctt tacggccgtg ccggctcccc
      661 aggagcgatc ctagcgcaag atgacgcacc ttcaacttac cgttccgttt aagttaaaga
      721 cggcgtcaac atacacgtaa gaatagaaaa tattccgtaa tatcagttag ttctgcggtt
      781 ttcttacgag atcggtttag catttccaca tcctgacccc acctctcggc ctgataaagc
      841 tcgtccaatt gagcggccgc gagggcctgc tctgcactga cgtgcctgtc cgaaagcgcg
      901 agtgcgacga tcgccgagcc cagcaatgtg gccgccgcat gcagccccgc gagttcgaaa
      961 tcgtcatact gggcgatagc gccctccagt gcctccaggg tctgtggcgg ctgttcgaca
     1021 tgcacgatgc ccgcgccgca aacgagagtc gcgccatagc gcgtgcgggc ccagtccaga
     1081 agcgggtccc attctgcggc ctgccgccga accagatttt ccggcccttg cgcccggtag
     1141 cagacgagat cgcttctcgc gaaggcgaga atttgctcga tggcgatctg acggttggga
     1201 cgcaccagat cgatggcagt gttcgcgagc gtggtgagaa tcatggtctc gggccggatc
     1261 gtatcggctt ggccgcgcca ctcctcagct atcgcctcgg caagcgcggc cgcgggtagc
     1321 gcaagcgctg caccagtcgg cgtgcgaatc gtcttcccgt ccaggagaac gctatatccc
     1381 ccgtccgtaa ccgcctcgac cgacacctcc cgatagaacc gcctcgcgtt cacagcttcc
     1441 tggaatccgc aaacgggtcg gccgtgctgt tgggatcgaa tccgaacaga cgccaggcat
     1501 ggaccatgtg cggcgaaagg ggtgcggtcg cccgcaatcg tccgccctcg ggatgggcca
     1561 tgtcgatgga gcgtgcgtgg agatgcaggc ggtcttccag ctcccccagg cccttggcgt
     1621 tttcgccacc atatttgaag tcgcccacga tgggcgtgcc cagatgcgca agatgcgcgc
     1681 gcaattggtg cgtgcgtccc gtgacgggac gcagcgccac ccaggcatat tgattggccg
     1741 cggtccccat cacggcataa tgggtggttg cggatttggc atcctccgaa tcctcggtcg
     1801 caggcgccat gcgttcgtca cgcccatgtt tgccgaagcc tgcttctttg gcgagcgcca
     1861 ggcgcacaac gccctgatgc gggatcggaa cgcccctggt cagcgcccaa tagatcttgc
     1921 gcgcgtcgcg acgacgcaag gattgggcca atgccgccgc cgcgggcagc gtgcgcgcga
     1981 caaccagaac accggacgta tcgcggtcaa gccgatgcac caggcgagga cgttgtttct
     2041 ttttgccctg tagcgcctca agcagcccgt ctatgtggcg gctgatgccc gaaccgccct
     2101 gcgtcgcgat cccgcttggc ttgttcagca cgaacaccga cgggtcttca tggatgacaa
     2161 gccgctccat gaaatcctta tcggcatcgc tgaaactgtg agcccgtggc ccattcggct
     2221 gcgccccggt ctggccctgg agctgcggcg ggatgcggat cgcctgccct gcctccagcc
     2281 ggtcgcccgc atgggcgcgc ttgccgtcga tgcgcacctg gcccgtgcgc aggagctttt
     2341 ccagcggccc atggccaatt gcggggtaat ggcgtttgaa ccagcggtcc aggcgcaggc
     2401 ccgcttcgtc ctcttgcacg gctgtcgtgg cgctcatgcg aggctgcgaa cgagccataa
     2461 gccgctgaac agcgcaatca ccgagagcaa caccgacgcg atcatataga ccgccgctcc
     2521 gatccaatcc ccgcgttcga tcagaagcgc ggcatccagc gaaaaggtcg agaatgtggt
     2581 gaagccgccg agaatgcccg tggtgagaaa g
//
LOCUS       DASYEG010000120         3042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2646325, whole genome shotgun sequence.
ACCESSION   DASYEG010000120 DASYEG010000000
VERSION     DASYEG010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2646325"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VGM72_05060"
     CDS             <1..400
                     /locus_tag="VGM72_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY4264665.1"
                     /translation="QNDYDTDPRLQEINLGAWDGLTDAQAKALDPVAFERRGADKWNV
                     RVPGGGENYADVAARAESWIQEIKADTFAVAHGAFTRILRGLFMGVDWKAMSNLDEKQ
                     GVLFRVRGNTIKRFDPPEALEKPTGPHPPG"
     gene            404..1000
                     /locus_tag="VGM72_05065"
     CDS             404..1000
                     /locus_tag="VGM72_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY4264666.1"
                     /translation="MPDFRDLTLYVIRHGECEHNALGVVAGQNDSPLTEKGRGHARAN
                     GVLLKSLAGDLSQFDFFASSLHRTCATMEIVRENAGLPRTGYVADRRLMEIDCGDNTW
                     RRWSDITTDAQKNPVWHRARWDYQHPGGESFHDLYRRVSEFLQTLKRDSIIVTHAGPV
                     RAIRGHYLGLTSDAVLSYETKHAGIMRLSAGTDATYGD"
     gene            1003..1602
                     /locus_tag="VGM72_05070"
     CDS             1003..1602
                     /locus_tag="VGM72_05070"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY4264667.1"
                     /translation="MTDFRDLTLYVIRHGECEHNVAGRAASWDDSPLTATGRAQARNN
                     GHLLEEIVGDLSRFDFFASPIHRTCNTMELVREGAGLPPTGYRADHRLMEIHFGDHTW
                     MTREEIAKLDPQLPGLDDQDDWDYVRPNGESWASLHERVGRFLPTLKRDAVIVTHYGP
                     ARAIRGHYLGLSAMQTKSYKPPHAGIMRLSAGAETYFGT"
     gene            complement(1693..1971)
                     /locus_tag="VGM72_05075"
     CDS             complement(1693..1971)
                     /locus_tag="VGM72_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264668.1"
                     /translation="MKFLLAAFAASAAMAVAPVAVQAADVQAAPAAQQQSALAPGSAA
                     GVKQAQTWHDNQTLYIVGGALVAGGVIWAVSSGGNNHHHTTTPSTGTL"
     gene            2168..>3042
                     /gene="aroC"
                     /locus_tag="VGM72_05080"
     CDS             2168..>3042
                     /gene="aroC"
                     /locus_tag="VGM72_05080"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY4264669.1"
                     /translation="MSFNTFGHLFRVTTFGESHGPAIGCVVDGCPPGVPLVESDIQAD
                     LDRRRPGQSKFVTQRKEPDTVRILSGVFRDERMKAQVTTGTPIALLIENVDARSKDYT
                     DIRDKFRPGHADYTYWAKYGVRDYRGGGRQSARETASRVAAGAIARKVLNALYKKVIV
                     RGALVQMGTQTIERARWKWEEVAKNPFFCPDPKAIVPWTQYLEAIRKKGSSVGAIIEV
                     VAEGIPVGLGAPIYGKLDADLAGALMSINAVKGVEIGDGMAVASLTGEQNADEIRMGK
                     GGKPKFLSNHGGGVMG"
BASE COUNT          631 a          932 c          943 g          536 t
ORIGIN      
        1 gcaaaacgac tacgataccg acccgcgcct tcaggagatt aatctgggcg cgtgggatgg
       61 actgacggat gcgcaggcca aggcgctcga tcccgtagcg ttcgaacggc gcggcgccga
      121 caaatggaac gtgcgtgtgc cgggcggcgg cgagaattat gccgatgtcg cggcgcgcgc
      181 ggaaagctgg atacaggaaa tcaaagcaga caccttcgcc gtggcgcatg gtgcgttcac
      241 acgcatcctg cgcggcctgt tcatgggcgt ggactggaaa gcgatgtcca atctggatga
      301 gaagcagggc gtgctattcc gcgtacgcgg caacacgatc aagcggttcg atccgccgga
      361 agcgctggaa aagcccaccg gcccgcaccc accgggttaa gacatgcccg attttcgcga
      421 cctgacgctc tatgtcatcc gccacggcga atgcgagcac aatgcgctgg gcgtcgtcgc
      481 gggccagaat gattctccac tgaccgaaaa gggacgcggc catgcgcgcg ccaatggggt
      541 gctgctcaaa tcgctggcgg gcgatctttc ccaattcgat ttcttcgcca gctcgcttca
      601 ccgcacctgt gcgaccatgg agattgtgcg cgagaacgcc ggcctgcccc gcaccggcta
      661 tgtcgccgac cggcggctga tggaaatcga ttgcggcgac aacacctggc ggcgctggag
      721 cgacatcacg acggatgcgc agaaaaatcc tgtatggcac cgcgcacgct gggattatca
      781 gcaccccggc ggcgaaagct tccacgatct ctacagacgt gtctccgaat tcctgcaaac
      841 gctcaagcgc gacagcatca tcgtgaccca cgccggcccg gtgcgcgcga tccgcgggca
      901 ctatctgggg cttacctctg acgcggttct gagttacgag acaaaacatg ccgggatcat
      961 gcgattgtcc gccggcacgg acgccactta cggcgattga acgtgaccga ttttcgcgac
     1021 ctcaccctct atgtcatccg ccacggcgag tgcgagcaca acgtcgccgg gcgcgcggca
     1081 tcgtgggatg actcgccgtt gacggcgaca ggccgcgcac aggcgcgcaa caacggtcat
     1141 ctgctggagg aaatcgttgg cgatctttcc agattcgatt tcttcgccag cccgatccac
     1201 cgaacctgca acacgatgga actggtgcgc gaaggcgcag gcttgccgcc caccggctat
     1261 cgcgccgatc accgccttat ggaaatccat ttcggcgacc acacatggat gacacgcgag
     1321 gagatcgcaa agctcgatcc gcaacttccc ggtctcgacg atcaggacga ctgggattat
     1381 gtgcggccaa atggcgaaag ctgggccagc ctgcacgagc gcgtcggacg ttttctcccg
     1441 accctgaaac gcgatgccgt gatcgtcacg cattacggtc cggcacgtgc gatccgcggg
     1501 cattatctcg ggctctcggc gatgcaaacg aagagctaca aaccgccgca cgccggaatc
     1561 atgcggctct ctgcgggcgc cgaaacatat ttcggcacct gaccggcgca aggatgttct
     1621 aaaaagaaaa aacctccgcc cgccgggatc gcgatccacg gcgaacggag gttctgttcc
     1681 gtcttcgaat tcttagagcg taccggtgct cggcgtcgtc gtgtgatggt gattgttacc
     1741 accgctggac accgcccaga tcacgccacc ggcgacgagc gcgccgccga cgatgtagag
     1801 cgtttgattg tcatgccagg tctgggcctg cttcacaccg gcagccgaac cgggcgcgag
     1861 cgcggattgc tgctgtgcag cgggagcagc ctgaacatcg gccgcctgaa cggcaaccgg
     1921 cgcaaccgcc atggcggcgc ttgcggcaaa ggcggcaagg aggaatttca tcggatattt
     1981 cctttaggat taagatgcaa aaactggcca gcccctcccc cgaattcgcc gggggtattc
     2041 gttaccgtcc tgtcctgtcg gttttaggcg actcgttggg ttaaacaaga gacaaatttg
     2101 ttacgtgcaa acttagtcgg tccccctgcg ttgtagccgc gcacgcaacc cgtggtatga
     2161 cgattccatg tccttcaata cgttcggcca tctgtttcgc gtaaccacct ttggcgagag
     2221 ccacgggccg gccattggct gcgtggtgga tggctgcccg ccgggcgttc cgctggtcga
     2281 atcagacatc caggcggatc tggatcggcg ccgccctggc cagtccaaat tcgtcacgca
     2341 acgcaaagaa ccggacacgg tccgaatcct ttccggcgtc tttcgggacg aacggatgaa
     2401 agcgcaagtg accacgggca caccgatcgc gctgctgatc gagaacgtgg acgcgcgctc
     2461 gaaggattac accgacatcc gcgacaagtt tcgccccggc catgccgact acacgtactg
     2521 ggccaaatat ggcgtgcgcg attatcgcgg tggcggacgg caaagcgcac gcgaaaccgc
     2581 aagtcgcgtg gcggccggcg ccatcgcccg caaggttctg aacgcactct ataagaaagt
     2641 catcgtgcgc ggcgcgctgg tgcagatggg cacgcagacc atcgagcgcg cgcgctggaa
     2701 atgggaggaa gtggcgaaga acccgttctt ctgccccgac cccaaagcga ttgtgccgtg
     2761 gacgcaatat ctcgaagcga tccgcaagaa gggttcatcc gtcggcgcga tcattgaagt
     2821 ggtggccgaa ggtattccgg ttgggttagg tgcgccgatc tacggcaagc tcgacgcaga
     2881 cctcgcaggc gcgctgatga gcatcaatgc cgtgaagggc gtggagattg gcgacggcat
     2941 ggcggtggcg tcgctcaccg gcgaacagaa cgccgacgag atccgcatgg gcaaaggcgg
     3001 caagccgaaa ttcttatcca accatggcgg cggcgtgatg gg
//
LOCUS       DASYEG010000121         4074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1487677, whole genome shotgun sequence.
ACCESSION   DASYEG010000121 DASYEG010000000
VERSION     DASYEG010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487677"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VGM72_05085"
     CDS             <1..413
                     /locus_tag="VGM72_05085"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY4264670.1"
                     /translation="SPTKFWRGLTEVAGHPEWQDDARFTKKRDRQENYRVLHRLLAEI
                     FATRERDHWLALLQARDVPSAAINTLDQVFADPQVKHLGLIREVPHPKLGHVSLVGGG
                     VNLSETPPEIRSAAPDLGQHNEEVLARIAMAKTT"
     gene            410..1591
                     /locus_tag="VGM72_05090"
     CDS             410..1591
                     /locus_tag="VGM72_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264671.1"
                     /translation="MIAPSQRLRNACAIVGAGNSRLGQVPGVSSLDLLIEAMANALDD
                     AGLKPADIDGIIVRGPDDSYCHHQQAGERLGINARFSTSLDNGGASQILSVAIAVMAI
                     EAGLATTIICGYGRDAWSRTHKSDAARVKNQTRPDDQHAQEFGPEYGYFGAVAAHAFG
                     AQRHMHEFGTTRENFAEIAVAFREHAMRNPDAQIRKPLSTADYFKARLIVSPFGLFDC
                     SLRSDGAGAVIVTRSERARDFKAPPVMIRGFGSFNNLTGWFAADNMIRTAARQSGEAA
                     YRMAGLGPSDIDTAQLYDCFTYMVLVQLEDYGFCAKGEGGPFVQSGALRMGGRLPTNT
                     SGGQLSEAHVEGMLQIVEGVRQLRGTYPAERQVAHANVALISGHGGNQVCHSTLILER
                     G"
     gene            1558..2010
                     /locus_tag="VGM72_05095"
     CDS             1558..2010
                     /locus_tag="VGM72_05095"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEY4264672.1"
                     /translation="MPFNADPGAWLMVPDTPLSKPVPRPSPESRSFWDGAKDRRLMLP
                     RCNVCGQFWFPPSQRCRHCLAADFAWQESAGRGRIYSFVVYHRVYHPAFENDVPYVVA
                     IVELDENVRLLTNIVGTPPEDVRCDARVRVVFADTDDGAVLPKFEIAP"
     gene            2007..3710
                     /locus_tag="VGM72_05100"
     CDS             2007..3710
                     /locus_tag="VGM72_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY4264673.1"
                     /translation="MMARGAKAFPRFSPLVLGLILVLLVLILPPVIYLIRSSLQEIAF
                     DGSFGAFTLEHYRALIGTGGLFLVILTSAAYAAGSAVVALLFGGVQAWIVERTDTPLR
                     SLVIIVSIVSLGIPSVLYTISFLLLLGKTGPLNQLLMGITGATEPLFNVYSLHGMILI
                     QGIEYTPLCFLLLSSVFRSTDAAFEEASMMSGAGIATTFRRITFKLVTPGIAALLILI
                     FIRAFEAFETPALVGLPGRVHVITNDIYQTMLDIPPNYGEASAFSVVMLVIMVFLLRL
                     YGNFSKHAEKYQTISGKGFRPRVMHIGPWRWLTMAVLVGLFFLLIVFPVGITLWVSLV
                     PYYDGVNAAALARFSLANFRTILFETSSASSLGNTFILGGATATLVAAFTTLAAWLAV
                     RRQPGAWLLDQLASAPLVFPAIVLAVAFLQFVLSVPFGLYGTLTSIIIASMVQFMPFG
                     MRYSYAGVLQIHRELEEASALSGARAPTTFLRVVLPLIAPAVVTCWLFIFLMVSKAVS
                     IPLLLAGPNSQVVAVSMFDMWQNGVAPELAALGIIWTIIMTVVSTAFFTVSRRYGLSA
                     R"
     gene            complement(3712..>4074)
                     /locus_tag="VGM72_05105"
     CDS             complement(3712..>4074)
                     /locus_tag="VGM72_05105"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY4264674.1"
                     /translation="EYWITLTTAQHLPIIAAGKGAPIAAQVLQPMPSTVASIMIPKGT
                     KHPHAAMLFIDYMLSEAGQTLLARAQYFPVNERVQPLKELQPVSPRLAGLKENFIDDE
                     VLYATHAKVAALLKKYFS"
BASE COUNT          672 a         1263 c         1254 g          885 t
ORIGIN      
        1 cctcacccac aaaattctgg cgcggcctga ccgaggtcgc gggccatccc gaatggcagg
       61 acgatgcgcg ctttacgaag aaacgcgatc gccaggaaaa ttaccgcgtg ctgcaccgat
      121 tgctggcgga gattttcgcg acgagagagc gcgatcattg gctggcactg ctgcaggcgc
      181 gcgatgtccc gagtgccgcc atcaacacac tggatcaggt gttcgccgat ccgcaagtga
      241 agcatctggg tctcattcgc gaagtccccc atcccaagct cgggcatgtg tcgctggtcg
      301 gaggtggggt gaatttgagc gaaacgccgc ccgagattcg cagcgccgcc cccgatctcg
      361 gtcagcacaa tgaagaagtg cttgcccgca ttgccatggc gaagaccaca tgatcgcgcc
      421 ctcgcaacgc ttgcgcaatg cctgcgccat tgtcggggcc ggcaacagcc gcctgggaca
      481 ggtgcccggc gtttccagcc tcgatcttct gatcgaggcc atggccaatg cgcttgacga
      541 tgcggggctg aagcctgccg atatcgatgg catcatcgtt cgcggaccgg acgattccta
      601 ttgtcatcat cagcaagccg gcgagcggct cgggatcaat gcacgcttct cgacttcgct
      661 cgacaatggc ggcgccagcc agatcctgtc tgtggccatc gccgtgatgg caatcgaagc
      721 gggtctcgcg accacaatca tttgcggcta tggccgcgat gcctggtcgc gcacgcacaa
      781 atccgacgcg gcgcgcgtca agaaccagac ccggcccgac gatcagcacg cgcaggaatt
      841 cggtcccgaa tatggctatt tcggcgccgt ggcggcgcac gccttcgggg cgcagcgtca
      901 catgcacgaa ttcggcacca cgcgcgagaa tttcgccgag atcgcggtcg cgtttcgcga
      961 acatgcgatg cgcaatcccg acgcgcaaat ccgcaagccg ctcagtaccg cggattattt
     1021 caaggcccgc ctgatcgttt ctcctttcgg tctgttcgat tgttcgctca gaagcgatgg
     1081 ggcgggtgcg gttattgtga cgcgcagcga gcgggcccgc gattttaaag cgccccccgt
     1141 gatgatccgc ggcttcggca gcttcaacaa tctgaccggt tggtttgccg ccgacaacat
     1201 gatccgcacc gccgcgcgcc agagcggcga ggccgcctat cgcatggcgg gtcttgggcc
     1261 gtccgatatc gataccgcgc agctctatga ttgtttcacc tacatggtgc tggtccagct
     1321 tgaggattac ggcttctgcg ccaagggcga gggtggccct ttcgtgcaat cgggcgcgct
     1381 cagaatgggc ggccggcttc ccaccaacac atcgggcggg cagctctcgg aggctcacgt
     1441 cgaaggcatg ttgcagatcg tcgaaggcgt gcggcaattg cgcggaacct atccagcgga
     1501 gcgccaggtc gcgcacgcga atgtggcgct gatctccggc catggcggaa accaggtgtg
     1561 ccattcaacg ctgatcctgg agcgtggctg atggtgcccg atacgcccct ctcaaaaccg
     1621 gtgccgcggc catccccgga atcgcgcagc ttttgggacg gcgcgaagga caggcgcctg
     1681 atgctgcccc gctgcaatgt ttgcggccag ttctggtttc cgccgtcgca gcgctgccgc
     1741 cattgtctgg ccgccgattt cgcatggcag gaaagtgcag gcagggggcg catctacagt
     1801 tttgtggttt atcatcgcgt ctatcacccg gccttcgaga acgatgtccc ctatgtcgtc
     1861 gccatcgtgg aattggacga aaatgtgcgg ctgctgacga atatcgtggg aacgccgccg
     1921 gaggatgtgc gctgcgatgc gcgcgtgcgt gtggtgttcg cggataccga tgacggtgcc
     1981 gtcctgccga aattcgaaat cgcgccatga tggcgcgtgg ggcgaaagct ttcccccgat
     2041 tctcgccgct ggttctcggc ctcatccttg tgttgctggt gctgattctg ccgccggtga
     2101 tctatctcat ccgttccagc ctgcaggaga tcgcgttcga cgggtccttc ggggccttca
     2161 cgctggagca ttatcgcgcg ctgattggaa cgggcgggct gttccttgtg atcctgacga
     2221 gtgccgccta tgcggcggga tcggcggtgg ttgcgctgct cttcggcggg gtgcaggcct
     2281 ggatcgtcga acgtacggat acgccgctac gttcgctggt catcatcgtt tccatcgttt
     2341 cgctcggcat accgagcgtg ctctacacga tttccttcct gctgctgctg ggcaagaccg
     2401 gcccgctgaa tcaattgctg atggggatca ccggcgccac cgagccattg ttcaatgtct
     2461 attcgctgca cggcatgatc ctgatccagg ggatcgaata cacgcccctg tgtttcctgc
     2521 tgctctcctc cgtctttcgc tccaccgacg cggcgttcga ggaagcctcc atgatgtcgg
     2581 gtgcggggat cgccacgaca ttccgccgca tcaccttcaa actggtgacg ccggggatcg
     2641 cggccttgct gatccttatc ttcatccgcg ccttcgaggc cttcgagacg cccgccttgg
     2701 tgggcctgcc ggggcgtgtg catgtgataa ccaacgatat ctatcagacc atgctcgaca
     2761 tcccgcccaa ttatggggag gcctcggcat tctcggtggt aatgctggtc atcatggtgt
     2821 ttctgctcag gctgtacggc aatttttcga agcatgcgga gaaataccag accatcagcg
     2881 gcaagggatt ccggccgcgg gtgatgcaca tcgggccctg gcgctggctg accatggctg
     2941 tgctggtggg gttgtttttc ctgctgatcg tctttcccgt cggcatcacg ctctgggtct
     3001 cgctggtgcc ctattatgac ggcgtgaatg cggcagccct ggcgcgtttc agcctcgcca
     3061 atttccgcac catcctgttc gaaacctcat cggcgtcgtc gctcggcaac accttcatcc
     3121 tgggaggcgc caccgcgact ttggttgccg cgttcaccac gcttgcggcg tggcttgcgg
     3181 tgcggcgcca gcccggcgcg tggcttctgg atcaactggc gagcgcgcct ttggtctttc
     3241 cggccatcgt cctcgcggtc gcgttcctgc aattcgtgct cagcgtgccg ttcgggctgt
     3301 atggtacgct gacctcgatc atcatcgcct ccatggtgca gttcatgccc ttcgggatgc
     3361 gctattccta tgccggcgtg ctgcagatcc atcgcgagct ggaggaggcc tcggcacttt
     3421 ccggcgcgcg cgcaccgacc acattcctgc gtgtcgtcct gccgctgatc gcgccggcgg
     3481 tggtgacctg ctggctattc atcttcctga tggtctcgaa agcggtgtcg attccactcc
     3541 tcctcgccgg accaaattcc caggtcgtcg cggtctcgat gttcgacatg tggcagaatg
     3601 gcgtcgcgcc cgagctggca gcactcggta tcatctggac catcatcatg acggtcgtga
     3661 gcacggcctt tttcaccgtc tcgcgccgct atggtctttc cgcgcgttga gctaggagaa
     3721 atatttcttc agaagcgcag ccactttcgc gtgggtggca tacaggactt catcgtcgat
     3781 gaaattctcc ttcagcccgg ccagacgtgg actgaccggc tgcaattcct tcagaggctg
     3841 cacccgttcg ttcaccggga aatattgcgc acgcgccagc agcgtctgac cagcctccga
     3901 cagcatgtag tcgatgaaca gcatcgcggc atggggatgc ttggtgcctt tcgggatcat
     3961 gatgctggcg acggtggacg gcatcggctg caacacttgg gcggcgatag gcgcgccctt
     4021 gccggcggcg atgatcggca gatgctgcgc ggtggtcagg gtgatccaat attc
//
LOCUS       DASYEG010000122         8685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1195603, whole genome shotgun sequence.
ACCESSION   DASYEG010000122 DASYEG010000000
VERSION     DASYEG010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 8685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8685
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195603"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1926)
                     /locus_tag="VGM72_05110"
     CDS             complement(<1..1926)
                     /locus_tag="VGM72_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264675.1"
                     /translation="MRLPHLDLPALALTGGGAVLAHPGGETRALSVAEARNLIAAGDV
                     LVCHAAFVAQRLHARLLKPLFDLLELFAFVCPGEPCLPSPLGLSRALGLGEAHIPADS
                     ARSLHVIAGALFDRLAGLSAEQKERTRLTASFLASAGWRWGPAILTVLGAPERTAGPL
                     SGIDAWRRLPEWEDQPPRSPPGSLPVDTEEAQARLAALIGHSGASRSQQRDFTTAATA
                     AFAPRNRVGAPRIALVEAGTGTGKTLGYLAPASLWAEKNGPGLWISTYTRNLQRQILQ
                     EAEKLERGARARPLKVVLRKGRENYLCLLNFEEAAKRNAHAPGQRAVALGLIARWLET
                     SPDGDLSAAGFPAFLAGTVPLRELTDRRGECIYAACPHYRVCFIERGLRRAHGADIVI
                     ANHALVMAQASHDGVRAEEADNITPERRVRYVFDEGHNLFDAADSAFAAELSGTEMAE
                     LRRWIRGPEGRRSRARGLNERARDLVADEDGASALEEAFAAAGALAGEGWLSRLGAGP
                     RGPGEVFLAHVYAHARARSADGDSEYGIEADVRPLEPELLDAAAKLDHWLVRLGAPLN
                     RLAGNLRARLDKEAGELESPMRVRIEAVLRGLERRAHLLIPAWRAMLAALEEEPHATS
                     DFADWFEIARIDGRDFDV"
     gene            complement(2268..4400)
                     /locus_tag="VGM72_05115"
     CDS             complement(2268..4400)
                     /locus_tag="VGM72_05115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4264676.1"
                     /translation="MIIEGGENHPLSKQNALLSGRIAIVYALSRYSFFLPFAALCMAA
                     CLMEQPGFFVLAIAPLFLLIGAALCSHRLKILYDRRDTDRDPRIWARRCTILSGLTGA
                     IWGLGALVWFVPGSFPAEAYLVLAFFGVSAIEFVARAAYRPAYLAHAAGSLVPLAALL
                     IHEGGAYQMLTAILVLFFGGTLYNYSGRVAELLDESILLRHDNAGLIRRLSEEKRAAE
                     RLCDAAQASERAKSVFVSNISHELRTPLNAIAGMAQLLERSDLVKAQRDHVKVLLESS
                     RGLKTLLDDIIALSQQGGEPLSPPEEGCDAVQAVRTVARLLQPNAWEKRLRLSVNIAS
                     GLSRAAADPRLFRRVLLKLAGNAIKFTERGNIELALDIADDETGAPCVRFAVADTGPG
                     IPHHLLAAIFDPFTKEDNSYATRHTGTGVGLAVAKRLVESIGGTIGVESEPAMGSRFW
                     IKVPTIKSSTIEEPIDEEHMTPPSGLSLLAYLPDETMRSSVERLLVPFGNSISHADTL
                     AESVTMSARGAYALILATAGSVDAFAAAPGQRTPILALAAAEERHPNGADAVLRWPAG
                     SNALYAAITGVTGEAGKYADGVKQDHIEAAIDAKAISDLEKSLGFKTLIDILQSYMHT
                     ADELAAALSVTSDKEDWSQAGRLAQDFAGAAGGLGLSALTSAARLLAQSARDGADDAA
                     LSTAAEGVLSEHSRVREALCRLYPDLAA"
     gene            4525..6132
                     /locus_tag="VGM72_05120"
     CDS             4525..6132
                     /locus_tag="VGM72_05120"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001968.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY4264677.1"
                     /translation="MTSGSRREAASAARAWPFEEARKLLARLEKRQAAGTARTDILFE
                     TGYGPSGLPHIGTFGEVARTSWVRQAFETMSDVPTRLVAFSDDMDGLRKVPDNVPNQE
                     MLRDYLGKPLTAIPDPFGTHPSFGAHNNARLKTFLDRFGFRYEFYSATDCYRSGRFDT
                     ALLTVLSHYEKVRDVVLPTLGPDRRATYSPFLPLCPRTGRVLQVPVVAWDAQAGTIVY
                     EEDGRRNEVPVTGGHCKLQWKADWAMRWLALGVDYEMSGKDLIPSVELSTRIVRVLGG
                     EPPETLTYELFLDESGQKISKSKGNGLSVDEWLAYGPEESLALFMFQKPRAAKRLYFD
                     AIPRAVDDYLAFLSQYHRAGTEPAAALENPVWFLHVGTPPSENYPVSFALLLNLVSAS
                     GTHERDVLWGFIRAYAPDAGPKTHPGLDRLVTYALKYYEDFVRPQKRYRPATPKEREA
                     LSALADALENMAEERDAETVQAAVYEIGKTHGFEPLRDWFKAIYEVALGQEQGPRFGS
                     FAALFGCAQTAVLIRTALAGAFLPETV"
     gene            6204..6923
                     /locus_tag="VGM72_05125"
     CDS             6204..6923
                     /locus_tag="VGM72_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264678.1"
                     /translation="MSAAEPSPFELYRAGKYEEAIAAGEAANDGNGLAMAARAAFAQA
                     NLSDKPCLSCLQRVERLARRSIAKDPTHPEAFIYLAAGVGYEARIVGNLRAQFAHYPE
                     IAKEAIDHALAIAPNDDWSLAAAGAWHIEVVRNGGSVLARAVYGARADAGLEYFARAF
                     AADPNNLVIRFQYALSLSGYDFNTYEQKAAEALTATVMIEPRTNYEGVIKQRANRLLD
                     LLKAHKRSEYLMLVNHYQGYP"
     gene            7047..7193
                     /locus_tag="VGM72_05130"
     CDS             7047..7193
                     /locus_tag="VGM72_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264679.1"
                     /translation="MAEIRENYMPKERGFGGKMGSKKTLWVAIVVIIAAAAVGAFYMF
                     PQFW"
     gene            complement(7199..7834)
                     /locus_tag="VGM72_05135"
     CDS             complement(7199..7834)
                     /locus_tag="VGM72_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY4264680.1"
                     /translation="MLTHKQIWRAIDALAERHRLSPSGLAKIAGLDPTTFNKSKRETA
                     RRKPRWPSTESIAKVLQATGSTLEQFVTLVGAEDGARGTLKLVPLIGLAQAGSAGFFD
                     DAGFPAGAGWEEIAFPELGDPHAYALEVTGESMLPVYRDGDRIILSPQASTRRGDRVV
                     VKTLEGEIMAKQLARVTMRRVELQSFNPQFEDRVLDLTELAFIHRIIWASQ"
     gene            7957..8304
                     /locus_tag="VGM72_05140"
     CDS             7957..8304
                     /locus_tag="VGM72_05140"
                     /inference="COORDINATES: protein motif:HMM:NF017880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HEY4264681.1"
                     /translation="MIDWVYKIVRRAEWDEAEKTGIFTGSADDRRDGFIHLSSANQVR
                     TTYDKYFQSEGNLLLTAIESVRLGAALKWEVSRGGEKFPHLYAALPLVSVHSVLAIGR
                     TSGGRPIFSPEIP"
     gene            8427..>8685
                     /locus_tag="VGM72_05145"
     CDS             8427..>8685
                     /locus_tag="VGM72_05145"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY4264682.1"
                     /translation="MSVHKGSCHCGAVEFEVEGAFDTGFICDCSLCRRKGAIMARLPQ
                     GQFRLTKGEGDLTTYQWNTKIAKHYFCKHCGIYTHHQPRTAP"
BASE COUNT         1634 a         2688 c         2781 g         1582 t
ORIGIN      
        1 gacatcgaag tcgcggccgt cgatgcgcgc gatctcgaac cagtcggcga aatccgaggt
       61 cgcgtggggt tcttcttcca gcgccgccag catcgcgcgc caggcgggaa tgagaagatg
      121 ggcgcgccgt tcgagcccgc gtaataccgc ttcgatgcgc acgcgcatgg ggctctccag
      181 ctcgcccgcc tccttgtcca gacgggcgcg aagattgccc gccaagcggt tcagcggcgc
      241 acccagacgc acgagccaat gatcgagctt ggcggccgcg tccagcagct caggctcaag
      301 cgggcgtaca tcggcttcga tgccgtattc ggaatcgcca tcggcacttc gcgcgcgtgc
      361 atgggcatag acatgggcca ggaaaacctc ccccggcccg cgcggtcccg cgccaagacg
      421 tgagagccag ccttcgccgg ccagcgctcc ggcggcggca aacgcctctt cgagcgcaga
      481 cgcgccgtct tcatcggcga cgagatcgcg ggcccgctcg ttcaatccgc gggcgcgcga
      541 gcggcggcct tcggggcccc ggatccaacg gcgcagctcg gccatttccg tgcccgacag
      601 ttcggccgcg aaggccgaat ccgccgcatc gaaaagattg tgcccctcat cgaagacata
      661 gcgcacgcgc cgctcgggcg tgatgttgtc ggcttcttcg gcgcgcacgc cgtcatggct
      721 cgcctgcgcc atcacgagcg catgattggc gatgacgatg tccgcgccat gagcgcggcg
      781 caaaccccgt tcgatgaagc agacgcgata atgcggacac gcggcataaa tgcattcgcc
      841 gcgccggtcg gtaagctcgc gcaaaggcac cgtgccggcg agaaaggccg ggaagcccgc
      901 agccgaaagg tcaccgtccg gcgaagtctc gagccagcgc gcgatcagtc cgagcgcaac
      961 ggcgcgctga ccgggcgcat gggcgttgcg tttggcggcc tcttcgaaat tgagcaggca
     1021 gagataattc tcccggccct tgcgcagcac cactttcaac ggacgcgcgc gcgcgccccg
     1081 ctcgagtttt tcggcctcct gcagaatctg gcgctgcaga ttgcgggtat aggtcgagat
     1141 ccagagtccc ggcccgttct tctccgccca gagcgaggcc ggtgcgagat aaccaagcgt
     1201 cttgccggtg cccgttcctg cctccaccag cgcgatgcgc ggtgcgccca cccgattgcg
     1261 cggtgcgaag gccgcggtgg ccgccgtggt gaaatcgcgc tgctgggacc ggctcgcgcc
     1321 gctgtgcccg atcagcgcgg caaggcgcgc ttgggcttcc tccgtatcga ccggcagact
     1381 gccgggcggg gacctcggcg gctgatcttc ccattcggga agccgccgcc atgcatcgat
     1441 gccggaaagc ggtcctgccg tgcgctcggg cgcgccaaga acggtaagga ttgcaggacc
     1501 ccaacgccat ccggcgctcg ccaggaagga cgcggtcagc cgcgtgcgct ctttctgctc
     1561 cgcagaaagg cccgccagcc tgtcgaacag cgcgcccgca atgacatgca ggctgcgcgc
     1621 ggaatcggcc ggaatgtgcg cttcccccag gccaagcgcg cgtgagagcc ccaacgggct
     1681 cggcaggcag ggctcgcctg ggcacacaaa cgcgaagagt tcgagcaggt cgaacaaggg
     1741 tttcaagaga cgcgcgtgca aacgctgggc aacgaaggcc gcatggcaga caagcacatc
     1801 gcccgccgcg atgagattgc gtgcttcggc cacggagaga gcgcgcgttt cgcctccggg
     1861 gtgcgccagc acggcgccgc cgccggtcaa cgcaagcgcc ggcaaatcca aatgtggcag
     1921 gcgcaatgtc ggcggcctcg cgaatccgaa aagtcagcga atatgacgag ttcgccgttg
     1981 cgccgaaagg ccggaaagtg aggacgcgga ttggcagagt aagcggtcat cgaagcctac
     2041 ggacgcgatg ttcttccccg cttgcgcaat caagcgcggg cgcgtttgca aatctgcggc
     2101 ctaggttagg cagggggttc gtatttcggc cattgttgcg ctccatgagg ccacaattcg
     2161 tgcgttgtat cggggagacc ttggtggttc gggaatgttt ggcgtgagcg ttagccccgt
     2221 ccgcgcctct cagaccggct gccggggctc accctttttc tgaacattca cgcggcaaga
     2281 tcgggataga gccggcatag cgcttcgcgc acgcggctgt gttccgaaag cacgccttcg
     2341 gccgccgtcg aaagcgcggc atcgtcggcg ccatcgcgcg cgctttgcgc gagcagccgg
     2401 gcggcggaag tgagtgcgga aaggcccaac ccgcccgcgg cgccggcgaa gtcctgcgcc
     2461 agacgtccgg cttgactcca atcttcctta tcggatgtga ccgacagcgc ggccgccaat
     2521 tcgtcagccg tatgcatgta ggactggaga atatcgatca gcgtcttgaa gccgagtgat
     2581 ttttccagat cggaaatcgc cttcgcgtcg atggccgcct cgatatgatc ctgcttcacg
     2641 ccgtccgcgt atttgcccgc ctcgccggtg acgccggtga tcgccgcata cagcgcgttg
     2701 gatccggcgg gccagcgcag cacggcatcc gcgccgttgg ggtgacgttc ctcggcggcc
     2761 gcgagcgcga ggatgggcgt gcgctgaccg ggtgctgccg cgaatgcatc gacgctgccg
     2821 gccgttgcga ggatcagcgc gtaggcgccg cgcgccgaca tggtgaccga ttcggccagg
     2881 gtgtcggcgt ggctgatgga atttccgaat ggcacaagca ggcgttcgac gctggaccgc
     2941 atcgtctcgt ccggcaggta ggcgagcagc gagagcccgc ttgggggtgt catatgttct
     3001 tcgtcgattg gctcctcgat cgtcgaggat ttgatcgtgg gcaccttgat ccagaatcgc
     3061 gaccccattg cgggctcgct ctccactccg atagtgcctc caatggattc gaccagacgc
     3121 ttggcaaccg cgagccccac acccgtgccg gtgtggcgtg tcgcatagga attgtcttct
     3181 ttcgtgaagg gatcgaagat cgccgccagc aaatggtgcg ggatgccggg ccccgtgtcg
     3241 gcgacggcaa agcgcacgca tggagcgccg gtttcatcgt ccgcgatatc gagcgccaat
     3301 tcgatattgc cgcgttcggt gaatttgatc gcattgcccg ccagcttcag cagcacacgg
     3361 cgaaacagcc gcggatcggc ggcggcacgc gacaggccgg acgcgatgtt gacggaaaga
     3421 cgcaggcgct tctcccaggc gttgggctgt agcaggcgcg caaccgtgcg caccgcctgg
     3481 acggcgtcac aaccctcttc cggcggcgaa agcggttctc cgccctgctg cgacagcgcg
     3541 atgatgtcgt cgagcagcgt cttcaagccg cggctcgatt ccagcaggac tttgacgtgg
     3601 tcgcgctgcg ccttgacgag gtcgctgcgt tcgagaagct gcgccatgcc cgcgatcgcg
     3661 ttcagcggcg tgcgcaattc gtggctgata ttcgagacga agaccgattt ggcgcgctcg
     3721 ctggcctggg ccgcatcgca aagccgttcg gccgcccgct tttcctcgct caagcgccgg
     3781 atcagtccgg cattgtcgtg gcgcaggagg atgctttcat ccagaagctc ggccacgcgg
     3841 ccgctgtaat tgtagagcgt gccgccgaag aagagcacca ggatggcggt caacatctgg
     3901 taggcgccgc cctcgtggat gagcagcgcc gccagaggca caagagagcc cgcggcatgt
     3961 gcaagatagg ccggacgata ggccgcgcgc gcaacgaatt cgatggccga cacgccgaag
     4021 aacgcgagga ccagataggc ttccgccgga aaagagcccg gcacgaacca gacaagagcg
     4081 cccaagcccc agatggctcc ggtgagaccc gaaaggatgg tgcagcgccg cgcccagatg
     4141 cgagggtcac ggtccgtgtc gcgcctgtcg tagagaatct tcaaacggtg ggaacacagg
     4201 gccgcgccaa tgagcagaaa cagcggcgcg atggcgagca caaaaaaccc gggctgctcc
     4261 atcaggcaag cggccatgca gagcgcggcg aacggaagaa aaaacgagta gcggctgagc
     4321 gcgtaaacaa tcgcgatcct gccgctcagc aaggcgttct gtttcgaaag gggatggttc
     4381 tcaccacctt cgataatcac gaccactccc tcggacacga tttcagcgtc cgtttcgccg
     4441 gcccgttcgg gacccgcgtt tgaccttgtt tagggcagag cctagctttc gcgcctttcg
     4501 atacgcttaa catcaaggat cgccatgaca tccggctcac gccgcgaagc tgcttccgcc
     4561 gcccgtgcct ggcccttcga agaggcgcgc aaattgctgg cgcgcctgga gaaaaggcag
     4621 gccgccggca ccgcccgcac cgatatattg ttcgaaaccg gctatggccc ttcgggcctt
     4681 ccccatattg ggacattcgg cgaggtcgcg cgtacgagct gggttcgcca ggcgttcgag
     4741 acgatgtcgg acgtgcccac caggctggtg gcgttctccg acgacatgga cggactgcgc
     4801 aaggtccccg acaatgtgcc gaatcaggag atgctgcggg actatctcgg caagcccctg
     4861 accgccattc ccgatccctt cgggacgcat ccaagctttg gcgcgcacaa caatgcccgg
     4921 ctgaagacgt tcctcgaccg tttcggcttt cgttatgagt tctacagcgc gacggattgc
     4981 tatcgcagcg gccgtttcga caccgcgctt ctcaccgtcc tgagccatta tgaaaaggtg
     5041 cgcgacgtcg tcctgccgac attgggtccg gaccggcgcg cgacttacag ccctttcctt
     5101 ccgctttgcc cccgaaccgg gcgcgtgctg caggtgcccg tcgtcgcatg ggacgcacaa
     5161 gcgggcacga tcgtttacga agaagacggc agacgaaacg aggtgcccgt caccggcgga
     5221 cattgcaaac tgcaatggaa ggccgattgg gccatgcgct ggctcgcgct cggcgtggac
     5281 tatgagatgt cgggcaagga tctcatcccc tcggtggagc tgtcgactcg aatcgtccgt
     5341 gtgctgggtg gggagccgcc cgagacgctg acttatgaat tgttcctgga cgaatcaggg
     5401 cagaagattt cgaaatcgaa gggcaacggg ctctcggtcg acgaatggct ggcctatggg
     5461 ccggaggaaa gcctcgcgct cttcatgttc cagaagccgc gcgctgccaa acggctgtat
     5521 ttcgacgcca tcccgcgcgc ggtggacgat tatctcgcct tcctgtcgca atatcatcgg
     5581 gctggcacag agcctgcggc ggcgttggaa aatcccgtct ggtttcttca tgtgggcacg
     5641 ccgccgagcg agaattatcc cgtcagcttc gcgctgcttt tgaatctcgt gagcgcatcg
     5701 ggaacccatg agcgcgacgt gctgtggggt ttcatccgcg cctacgcgcc ggacgcagga
     5761 cccaagacac atcccggact cgaccggctc gtgacctatg cgctcaaata ttacgaagat
     5821 ttcgtgcggc cgcagaagcg ctatcgcccg gccacgccca aggagcgcga ggcgctgtcc
     5881 gcgctcgccg atgcgctgga gaacatggcc gaggaaagag acgccgaaac cgtgcaggct
     5941 gcggtctatg agatcggcaa gacccacggt ttcgagccgt tgcgcgactg gttcaaggcg
     6001 atctacgaag tggcactggg ccaggagcag gggccgcgct tcggttcgtt cgcggcgctg
     6061 ttcggatgcg cgcaaaccgc ggtgttgatc cgcaccgcat tggcgggcgc gtttcttccc
     6121 gaaacggttt agaggtcaag gtgcggcgac tgggcgcggc gtgcattctg gcgggtttgc
     6181 tgtggggccc aggatgcagc cccgtgtccg ccgccgaacc gtcgccgttc gagctttacc
     6241 gcgccggaaa atatgaagag gcgatcgcgg ccggagaagc cgccaacgac ggcaatggtc
     6301 ttgcgatggc cgcccgcgcc gctttcgccc aggccaatct cagcgacaag ccctgtcttt
     6361 cctgcctgca gcgggtcgaa cgtttggcgc gccgctcgat tgcgaaggac ccgacccatc
     6421 ccgaggcgtt catctatctg gcagcgggcg tgggctatga agcacgcatc gtcggcaacc
     6481 tgcgtgcgca gttcgcgcat tatccggaaa tcgcgaagga ggcgatcgat cacgctctgg
     6541 ctatcgcccc gaacgatgat tggagcctgg cggccgcggg cgcctggcac atcgaggtgg
     6601 tgcgcaatgg cggcagcgtg cttgcgcgcg cggtctatgg ggcgcgtgcc gatgcgggac
     6661 tggaatattt cgcccgcgcc ttcgcagccg atccgaacaa tctcgtgatc cgcttccaat
     6721 atgcgctgtc cctgtcaggt tacgatttca acacttatga acagaaagcg gcggaagcgc
     6781 ttaccgccac ggtgatgatt gaaccccgca caaattatga aggcgtcatc aagcaacgcg
     6841 cgaaccggct gctcgatctg ttgaaagccc ataaacgaag cgagtatctg atgctcgtca
     6901 atcattacca gggctatccg taaaaccctc ttaccttccc gtttggcact tcagcttggc
     6961 ggtttgcccg cgcatgcggg aaccatagct gtgccgtcgc attgtcttgg acggcatcag
     7021 cgcgaaacga agcgcaggag gtagtcatgg ccgagattcg agagaattac atgcccaagg
     7081 aacgcggttt cgggggcaag atgggcagca agaagacgct gtgggtcgcc attgtcgtca
     7141 tcatcgcggc ggcggccgtt ggggctttct atatgttccc gcaattctgg taatagaatt
     7201 actggcttgc ccagatgatg cggtgaatga aggcaagttc ggtcaaatcc aacacccggt
     7261 cttcgaattg cgggttgaag gattgcagct cgacgcggcg catggtgacg cgggcgagtt
     7321 gcttggccat gatctcgcct tccagggtct ttaccaccac gcgatcgcct cgacgcgtgc
     7381 tcgcttgcgg cgagaggatg atgcggtcgc cgtcgcggta gacgggcagc atggattcgc
     7441 ccgtgacttc gagcgcatag gcatggggat cgcccagttc tggaaaagcg atttcttccc
     7501 atccggcccc agccggaaaa ccggcatcgt cgaaaaagcc cgcgctgccc gcctgcgcga
     7561 gaccgatcag ggggacgagc ttcagcgtcc cgcgcgcacc gtcttcggcc ccgaccagcg
     7621 tgacgaattg ctccagcgtc gagccggtcg cctgaagcac tttggcgatg gattccgtcg
     7681 aaggccaacg cggcttgcgc ctggcggtct cccgcttgct cttgttgaac gtcgtcggat
     7741 cgagtcccgc aatcttcgcc agacccgatg gcgacagcct atgacgctcg gccagggcat
     7801 cgattgcgcg ccagatttgt ttatgcgtta gcatggagtc cccctcccag ctcgcaaaaa
     7861 tgggataatt gtccggattc tgcgcctaaa ttcctattaa taaggttaat ataggattaa
     7921 ttacttattc atgttctgta tgcaaggtct gaggtcatga tcgactgggt ctacaagatc
     7981 gttcgccggg cggaatggga tgaagccgag aagaccggaa ttttcacagg atcggccgat
     8041 gaccgacgcg acggtttcat tcacctgtcc tcggcaaatc aggtgagaac aacctatgat
     8101 aagtacttcc aaagtgaggg caacctttta ttgacggcga tagaatcggt tcgcctgggc
     8161 gccgcactga aatgggaagt gtcccggggc ggcgagaaat tcccccatct ctatgcggcg
     8221 ctcccgcttg tctctgtcca tagtgtattg gcaattggcc gtacgtccgg cgggcggcct
     8281 attttttcgc cggaaattcc ctgagacagt gatgaggcgc atggcctcaa ccttgatgca
     8341 gggcgtcgcc gtgacctaca tgaggggaac aaaggcgtct gagccacgaa gcgcggtgag
     8401 gcgcataaca tatttcggag atggaaatgt ccgttcacaa aggctcctgt cattgcggcg
     8461 cggtggaatt cgaagtcgag ggcgcgttcg ataccggctt catctgcgat tgctcactgt
     8521 gccggcgcaa gggcgcgatc atggcccgcc tgccgcaagg gcagttccgg ctcaccaagg
     8581 gcgaaggcga tctgaccacc tatcaatgga acacgaaaat cgcgaagcat tatttctgca
     8641 agcattgcgg gatctatacg catcatcagc cgcgcacggc gccgg
//
LOCUS       DASYEG010000123        19135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_5113195, whole genome shotgun sequence.
ACCESSION   DASYEG010000123 DASYEG010000000
VERSION     DASYEG010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 19135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19135
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5113195"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VGM72_05150"
     CDS             <1..400
                     /locus_tag="VGM72_05150"
                     /inference="COORDINATES: protein motif:HMM:NF027523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264683.1"
                     /translation="TLGTVGHVSALRRLKVGPFSIESAISLETLEGFGHSPAALEHLR
                     PIATALDGILALAVTEPDSVRLRSGNPILIRAPHFQRLVEQGGMTGTQTDLQGLTVFL
                     STARGEPVALAEVAQGELRPFRVFTFGPSV"
     gene            434..703
                     /gene="rpsO"
                     /locus_tag="VGM72_05155"
     CDS             434..703
                     /gene="rpsO"
                     /locus_tag="VGM72_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY4264684.1"
                     /translation="MSVTTERKKEVITQYATGENDTGSPEVQVAVLSERITNLQDHFK
                     THAKDNHSRRGLIKMVSQRRRLLDYVKSKDEARYQTLIQRLGLRR"
     gene            871..3012
                     /gene="pnp"
                     /locus_tag="VGM72_05160"
     CDS             871..3012
                     /gene="pnp"
                     /locus_tag="VGM72_05160"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY4264685.1"
                     /translation="MFNIHREEIDWGGRKLVLETGRVARQADGAVLATYGETVVLATA
                     VGMTEPKQGIDFFPLTVNYQEKFYAAGKIPGGYFKREGRPTERETLISRLIDRPIRPL
                     FVPGYRNETQVIVTVLSHDLENEPDVVAMVAASAALTLSGIPFMGPIGAARVGYIGGA
                     YKLNPPIDEMTDSNLELVVAGTADAVLMVESEAKELSEEVMLGAVMFGHREMQAVIDA
                     IIRLAEKAAKEPRAVHTNEYEAELALLARHVEDGLKTAYQIAHKGARHERIDAIKSEA
                     LAALAGEEDGKIPPNLFGKLFKEMESRVMRGSVIDTGRRIDGRDLKTVRPIVSEVGIL
                     PRAHGSALFTRGETQALVVATLGTGEDEQFIDSLEGTYKQHFMLHYNFPPFSVGEVGR
                     MTGAGRREIGHGKLAWRALNPLLPDKANFPYTIRVVSEITESNGSSSMATVCGTSLAL
                     MDAGVPLKRPVAGIAMGLILEGAKFAVLSDILGDEDHLGDMDFKVAGTEGGVTSLQMD
                     IKITGITEEIMQVALNQAKDGRLHILGEMAKALTVARAELGEHAPRIETIKIPTDKIR
                     EVIGSGGKVIREIVEKTGAKIDIQDDGTIKVASADGESIKAALKWIKGIVAEPEVGEI
                     YEGKVVKIMDFGAFVNFFGPRDGLVHISELASKRVAKVNDVVKEGDTVKVKLIGVDDR
                     GKVRLSMKVVDQATGEDLSKKQGAEGEQQAG"
     gene            3114..3443
                     /locus_tag="VGM72_05165"
     CDS             3114..3443
                     /locus_tag="VGM72_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264686.1"
                     /translation="MIVFRLFGLLLIVLALMLLGADLVSTLEKNGETVIRSLDQILML
                     LGADVKPWLQEQLSPQFANVLLVALSWPGWAILGILGTLLAMLSAGHREPRAPLPPQP
                     PANPINR"
     gene            3553..3756
                     /locus_tag="VGM72_05170"
     CDS             3553..3756
                     /locus_tag="VGM72_05170"
                     /inference="COORDINATES: protein motif:HMM:NF021772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEY4264687.1"
                     /translation="MSIEAPETIVVDHTRVACEGSGGALGHPRVYLEMGERDFVECGY
                     CDRRFALSPEALEAREKGEAGAH"
     gene            3753..6656
                     /gene="polA"
                     /locus_tag="VGM72_05175"
     CDS             3753..6656
                     /gene="polA"
                     /locus_tag="VGM72_05175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEY4264688.1"
                     /translation="MNAPDHNVLGLRAISPGDHLYLIDGSGFIFRAYHALPPLNRKSD
                     GLPTGAVSGFCNMLWKLLEDSKAGDKPTHLAVIFDAGRKSFRNDMYTDYKANRSAPPD
                     DLVPQFPLTRDAVRAFGIACVEKAGFEADDLIATYARLAREAEARVTIVSSDKDLMQL
                     IYDGKVEMLDPVKNRRIGPAEVIEKFGVAPDKVIDVQALIGDSIDNVPGVPGIGVKTA
                     AKLIAEFGDLETLLARAGEIKQNKCREALIEHIEQARLSARLVRLDEAVPLDEEIDQF
                     AVRDPDPAKLMPFLKAMEFSNLLRRAAAKLGVEDAEAVSVTPSVSTVPQPQPQPSPPL
                     ADAKICPGAVMDRAALLKPIEYGGYECVTEIAALDRWVAAAHEAGHLAIALESDSIDP
                     MRARLFGLALATAPGRACYIPLAHRSGEGLALGGATPAQLTEEDVFARLGPLLENESV
                     LKIGHNLKFAVVLLGQRGLAMRALDDTLLLSYVLDIGLHGHGLEELSETHLQHRPIAL
                     SDIAGKGRDKISFEQVAIAEATRYGAERADIALRLHALLKPRLVAERRMTVYETLERP
                     LIGVLAEMERTGIAIAPSFLAKLSNDFAREMARLEEECCGHAGERFNLGSPKQLGDIL
                     FGKFSLPGGRRTKTGAWSTDADILEELAAQGHALPKTILQWRQLSKLKSTYTDALPTY
                     VNATTGRVHTSYALGATTTGRLSSNEPNLQNIPIRTEEGRRIRRAFVAPEGRCLVSAD
                     YSQIELRLLAHIADVPNLRQAFADGLDIHAMTASEMFGVPVKDMPAEIRRRAKAINFG
                     IIYGISGFGLANQLGIPRGEADAYIKKYFERFPGIRDYMESTKAFVREHGYVETIFGR
                     RIHIRDVKAASAATRAFGERQAINAPIQGSAADIIRRAMVRLPPALAENDLDAHLLLQ
                     VHDELVLEVADSQGSQVCDVARAVMEKAPLPVFQLRVPLVVDARAGKNWDEAH"
     gene            complement(6681..8417)
                     /locus_tag="VGM72_05180"
     CDS             complement(6681..8417)
                     /locus_tag="VGM72_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264689.1"
                     /translation="MSQVHFEIFRQQGKSGGWSLAEAMEDREAALERGRQMLEEGQAA
                     AVRVVKETYQASTGDYMSLTIFEGGHTEVKKKNKKVEDIATFSPCFKPDDLYSYHARM
                     TMTRVLGEWLGRQKLTVTELIHGAAALERFEATGTTYQHAIQKVAVAQAAESETTVSQ
                     IVKQLNELCTAAIHRVYKDEKRNLFPELQAGQFGPLASKLGANPEGRYILNGALAKYL
                     GHAKTWDTKLQRLLTLMEELPVEGPGRALLLTAIDTLVAEMLNGAAALADLLGHNPDL
                     GHALMNLVELFLGTLVNAAEGAGHGINDLARSFAKDELPEARAAIAGRILAELKGLKR
                     LCPSSLDEEFKMLRRLGNRLVRGQGKYLSNQDLIGAFTERSRRLVSQEPLQQFMQASK
                     SSDEKLERLLVVEENIVGAENKRMLSAFMMPIITSNNFEELLLAGAPVAQRLRRLAEI
                     QERVLRSGFQEVQKNQIAMALDTVAQRIEVRAKFLASIETRIANPIERAQTMLKLCAA
                     GIFTQGDLMMKARRLLMASLAKPGFLATYIAQLEHERRTGVDKDQAMGELAAQLESIG
                     IAPEDALRALAA"
     gene            complement(8461..9309)
                     /gene="moeB"
                     /locus_tag="VGM72_05185"
     CDS             complement(8461..9309)
                     /gene="moeB"
                     /locus_tag="VGM72_05185"
                     /inference="COORDINATES: protein motif:HMM:NF004281.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEY4264690.1"
                     /translation="MNEIPRSRPPLDDEELERYARHIVLREVGGAGQAKLLAAKVLVI
                     GAGGLGSPLILYLAAAGVGTIGIADFDRVSLSNLQRQIAHRTQDVGRLKTESARDAAH
                     AINPGVKMVPHAMRLTAANAADLIAGYDVVADGSDNFATRFLINDACYFAKKTLVSAA
                     VTEFDGQLATYKAWDGSGLYPCYRCLFAEPPPPETTTSCSEAGVLGVVAGIMGTLQAL
                     EVMKEILAIGETMAGRLLVYDALAAQSRTLKVPKDPGCALCGSAPTIRDLSTHAAPAE
                     ACLTGI"
     gene            9736..11562
                     /locus_tag="VGM72_05190"
     CDS             9736..11562
                     /locus_tag="VGM72_05190"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEY4264691.1"
                     /translation="MAVPTTPTRNKKLLAWVEEVAAKCTPDRIYWCDGSQAEYDRLCA
                     EMVQAGTFIKLNEKKRPNSFLCRSDPADVARVEDRTFICSTDKTDAGPTNNWMNPKDM
                     RATLDRLFTGAMRGRTMYVVPFSMGPLGSPISHIGVQLTDTPYVAVSMRIMTRMGQQA
                     LDVLGDGDFIPCLHSVGAPLAPGQKDVAWPCNAEHKYIVHFPDDYSIHSYGSGYGGNA
                     LLGKKCFSLRIASLMGRDQGWLAEHMLILGVESPQGEKTYVAAAFPSACGKTNFSMLV
                     PPNGFDGWKVSTIGDDIAWIKPRPDGTFRAINPEAGFFGVAPGTGMKSNPNAVKMLNR
                     DVIFTNVALTDDGDVWWEGLTDEPPAHLIDWQGKDWTPNSGRKAAHPNSRFTASARQC
                     PSFDPAGDDPEGVPISAFIFGGRLSKTFPLVYEARNWQNGVYWAATMGSEATAAAVNQ
                     AAIRRDPFAMLPFCGYNMGDYWKHWLAIQDKAKKLPRIYRVNWFRRDDNGKFVWPGFG
                     ENMRVLKWIVDRANGRAKDSPEGPFGFMPRYQDLHWTGMEFSKDKFHRITDIDRAEGL
                     EEAEEQSEHFGRFGDRLPTQLEAERQDLIRRLKAAPPVWTTG"
     gene            11807..12256
                     /locus_tag="VGM72_05195"
     CDS             11807..12256
                     /locus_tag="VGM72_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEY4264692.1"
                     /translation="MALKNFTLTAAALIALTSASFAANTTTGTSASKATTEVEKAVGA
                     GDTLSATDLKSAVKLGTLKDAKDKLATAKVDDPMGNIIGPVKDVVTGKTGAPTAIHVD
                     VGGWLGVGERVVSINAGNFTYIPSRDILLTKMSKADIQKLPAVKQKS"
     gene            complement(12409..13533)
                     /gene="dnaJ"
                     /locus_tag="VGM72_05200"
     CDS             complement(12409..13533)
                     /gene="dnaJ"
                     /locus_tag="VGM72_05200"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY4264693.1"
                     /translation="MSKRCYYEILGVARTCAAEEVKGAYRKLAKECHPDRNPGDHTAE
                     HKFKELSEAYEVLKDPDKRGAYDRFGHAAFENGGRGGPGGFDFSASFTDVFDDLFGEF
                     MGGKRNRRSNRGGDLRYNMEITLDDAYRGKAAEIRVPSLIACEECTGTGAKPGTSPET
                     CATCAGHGKVRATQGFFTIERTCPKCRGAGRVVKHPCKACGGHGHVQKERTLSVDIPP
                     GVEEGTRIRLSGEGQAGLNGGPPGDLYIFLSVQQHPIFQRDAHDLYCRVPIGFVTAAL
                     GGEIEVPTLSGAGARIAIPEGAQTGHQFKLRGKGMPVLKSGGHHGDLFVEVRVETPVK
                     LTRKQKELLRSFETETPQGCNPDSESFFAKMKEFLSGGAD"
     gene            complement(13685..15616)
                     /gene="dnaK"
                     /locus_tag="VGM72_05205"
     CDS             complement(13685..15616)
                     /gene="dnaK"
                     /locus_tag="VGM72_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY4264694.1"
                     /translation="MAKLIGIDLGTTNSCVAVMEGSAPKVIENSEGGRTTPSIVAFTP
                     EGERLIGQPAKRQAVTNPEHTFFAIKRLIGRRFDDPMTQKDIGMVPYKIVKAGNGDAW
                     VQGRESKQYAPSEISAFILQKMKETAESYLGEKVSQAVITVPAYFNDAQRQATKDAGK
                     IAGLEVLRIINEPTAAALAYGMDKKNAGTIAVYDLGGGTFDISVLEIGDGVFEVKSTN
                     GDTFLGGEDFDIRLVNYLADEFKKESGIDLRSDRLALQRLKEAAEKAKIELSSAMQTE
                     VNLPFITADASGPKHLTMKITRAKLEALVDDLIQKTIAPVKQALKDAGVSANQIDEVI
                     LVGGMTRMPKVQDAVKQLFGKEPHKGVNPDEVVAIGAAIQAGVLKGEVKDVLLLDVTP
                     LSLGIETLGGVFTRLIERNTTIPTKKSQIFSTADDNQTAVTIRVFQGEREMAANNKLL
                     GQFDLVGLPPAPRGVPQVEVTFDIDANGIVSVGAKDKATGKEQTIRIQASGGLSQADI
                     DKMVKEAEAHAADDKQTRELAEAKNHGDALIHSTEKAAAEHGSKVSDAERSAIENAIA
                     ALKAAIKEDNAEDIKAKTNALAQASMKLGEAMYKAQSGGGDGAEGPGAGPDGDGAPKD
                     ENVVDAEFSEVDDQKKGAA"
     gene            complement(15895..16143)
                     /locus_tag="VGM72_05210"
     CDS             complement(15895..16143)
                     /locus_tag="VGM72_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264695.1"
                     /translation="MRYLILMASVGLALCGCATDRNRADRLAIANCNAVGITEKDPQF
                     ATCREAYSREYVEGRLEHDYHDALTAFHDPKLAHYDPY"
     gene            complement(16257..16511)
                     /locus_tag="VGM72_05215"
     CDS             complement(16257..16511)
                     /locus_tag="VGM72_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264696.1"
                     /translation="MRVSIAGIIGILGLCGCATDESVYRQANLECQAVGISEKDPQFA
                     TCSAAYSRQHLENQLDRSYRDALKRVPNELDYRIPHQDVY"
     gene            complement(16618..17070)
                     /locus_tag="VGM72_05220"
     CDS             complement(16618..17070)
                     /locus_tag="VGM72_05220"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY4264697.1"
                     /translation="MHVISNYVALILAALFGAIGLVHLAGPRFVREAYERWEYSQNLR
                     RIVGFLEIIAAAWLADPVFRLWGIGLAAAINFGGVVTLLNHRQYLHAGPGLVMMLALL
                     PATLAVPGAHPVRFIAIMPAPVAQQQATPRQDDLEAARDDARAQPSGG"
     gene            17296..17790
                     /locus_tag="VGM72_05225"
     CDS             17296..17790
                     /locus_tag="VGM72_05225"
                     /inference="COORDINATES: protein motif:HMM:NF018960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY4264698.1"
                     /translation="MITQGLMIPDSYARLASIAKCRTELQALFRFWEKLRGPRSMPAR
                     RDFDPADLRALLPHLILIDVFAQKPPERRFRVRLQGTAQVDYQGSDWTGCYLHEKTEQ
                     ASADRLCAVGDHIVATHEPWMSTGGLYWTRRKPYSRFESILLPLSDDDATVNMILGLT
                     IFSA"
     gene            complement(17913..18185)
                     /locus_tag="VGM72_05230"
     CDS             complement(17913..18185)
                     /locus_tag="VGM72_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264699.1"
                     /translation="MLELLVLHVQPVMGQPAPPLLVPGVSNEKLATVIGVAEAEPENS
                     PKLAAKATPTATRADLTVLIDMSPCFFPMITQAPAANSNNCARIFK"
     gene            18184..18702
                     /locus_tag="VGM72_05235"
     CDS             18184..18702
                     /locus_tag="VGM72_05235"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY4264700.1"
                     /translation="MGVYVPVTTQFPSGANGLSGGAIVPDGTQAAYLALTNGFISQNT
                     AALIANSTTYTLSVFVGRRNDFNPAISGSIALTNNGTVFATLAMSAPAQGQWLDETVS
                     YTTTLADSLLFGGTLGIRITDLNGNQLNVDDVRLSQSTTTVTDVPEPATLGLFGLGLA
                     AVGVARRKRRAA"
     gene            complement(18730..>19135)
                     /locus_tag="VGM72_05240"
     CDS             complement(18730..>19135)
                     /locus_tag="VGM72_05240"
                     /inference="COORDINATES: protein motif:HMM:NF015882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEY4264701.1"
                     /translation="ITPEEVRQVRGDAIIATGRSDYPNQINNVLGFPYIFRGALDVAA
                     TTINEEMKIAAANALAELARETVPDEVAAAYQGARHAYGRDYIIPVPFDPRLISRVSS
                     AVAEAAMRTGVARRPILDMEAYRARLAKRLGG"
BASE COUNT         3632 a         5844 c         5896 g         3763 t
ORIGIN      
        1 aacgctgggg accgtaggac atgtgagcgc tctgcggcga ttgaaggtgg ggccattttc
       61 catcgaatcc gcgatttcgc tggaaacgct ggagggtttc ggccatagtc ccgccgcttt
      121 ggagcattta aggcccatcg cgaccgcgct ggacggcatc ctggcgctgg ccgtgacgga
      181 gccggattcg gttcgcctgc gaagtggcaa ccccatcctt atccgcgcgc cgcattttca
      241 gcggcttgtg gaacagggcg ggatgaccgg gacgcagacg gatcttcagg gtctgacggt
      301 gtttctttcc acggccaggg gcgagccggt tgcgctcgcc gaagtggcgc aaggggagtt
      361 gcgaccgttt cgggttttca ctttcggacc aagcgtttaa gacggcttgg cctcagcacg
      421 ggactttaat tcgatgtcgg tgacgacgga acgcaaaaaa gaagtgatca cgcaatacgc
      481 gaccggtgag aacgatacgg gttcgccgga ggtccaggtg gccgtgctct cggagcgcat
      541 caccaatctt caggaccatt tcaagaccca cgcgaaggat aatcattcgc gccgcggtct
      601 gatcaagatg gtgtcgcagc gccgccgcct gctcgattac gtcaagtcca aggacgaagc
      661 gcgctatcag acgctgatcc agcgtctggg tctgcgccgc tgatcgccgt tcttcaaacc
      721 ggctggacgc cagccgtgca cacacaccgg tattatccgg tctcagcttg cgggcgccca
      781 aacgcgcgtt cccgcgggca cagattttct tgaccacacc agcaaggaag gcccgcggca
      841 atccggccat gggaaagaag gaaaacaaca atgttcaaca ttcatcgcga ggagatcgat
      901 tggggcgggc gcaagctcgt tcttgaaacc ggacgggttg cgcgccaggc cgatggggcc
      961 gtgttggcga cctatggcga aacggtcgtg ctcgctacgg ccgtcggcat gaccgagccc
     1021 aagcagggca tcgatttttt ccccctcacc gtcaactacc aggaaaaatt ctatgccgcc
     1081 ggaaaaattc cgggcggcta tttcaagcgc gagggacgcc cgaccgagcg tgagacgctg
     1141 atctcgcggc ttatcgaccg tccgatccgg ccgcttttcg tgccgggcta tcgcaacgag
     1201 acccaggtca tcgtgaccgt gctgagccac gatctcgaaa acgaacccga tgtcgtggcg
     1261 atggtggcgg cctctgccgc gctcaccctt tccggcatcc ccttcatggg accgatcggg
     1321 gcggcgcgcg tcggctatat cggcggtgcc tacaaactga acccgccgat cgatgagatg
     1381 accgattcca atctcgaact cgtggtcgcc ggcaccgccg atgcggtctt gatggtggag
     1441 tccgaggcca aggagctttc cgaagaggtc atgcttggtg cggtcatgtt cggccaccgc
     1501 gaaatgcagg ctgtgatcga cgccatcatc cggctcgcgg aaaaggcggc gaaggaaccg
     1561 cgcgctgttc acaccaatga atacgaggcg gaactggcgc ttctggcccg ccatgttgag
     1621 gacggtctga agaccgccta tcagattgct cataagggcg cgcgtcatga acgcatcgac
     1681 gcgatcaaga gcgaagcctt ggcggcgctc gccggcgaag aagacggcaa gatcccgccc
     1741 aacctgttcg gaaaattgtt caaggaaatg gaaagccgcg tgatgcgcgg ttccgtcatc
     1801 gatacggggc ggcgcatcga cggccgcgat ctgaagacgg tgcgccccat cgtctccgaa
     1861 gttggcatcc ttccgcgcgc ccatggcagc gcgctgttca cgcgcggcga gacgcaggcc
     1921 cttgtggtgg cgacactcgg caccggcgag gacgagcaat tcatcgattc tctggaaggc
     1981 acctacaagc agcatttcat gctgcactat aattttcctc ccttctcggt gggcgaagtg
     2041 ggccgcatga cgggcgcggg ccggcgcgag atcggccatg gcaagctcgc ctggcgcgcg
     2101 ctcaatccgc tgctgccgga caaggcgaat tttccttaca ccatccgggt ggtgtcggag
     2161 atcaccgaat ccaacggttc gtcctcgatg gcgacggtct gcggaacgtc gcttgcgctg
     2221 atggatgcgg gcgtgccgtt gaagcgtccg gtggccggca tcgcgatggg cttgattctc
     2281 gaaggcgcga aattcgccgt cctttccgac atcctgggtg acgaggatca tctcggcgat
     2341 atggatttca aggtggcggg aaccgaaggc ggcgtcacct cgctgcagat ggacatcaag
     2401 atcaccggca tcaccgaaga gatcatgcag gtggccctca accaggccaa ggacggccgc
     2461 ttgcacatcc tgggcgagat ggccaaagcg ctcacggtcg cgcgcgccga actcggcgag
     2521 catgcaccgc gcattgaaac gatcaagatc ccgaccgaca agatccgcga agtgatcggt
     2581 tccggcggca aggtgatccg cgagatcgtg gagaagaccg gcgccaagat cgatatccag
     2641 gacgatggca ccatcaaggt ggcgagcgcc gatggcgaat ccatcaaggc ggcgctcaaa
     2701 tggatcaagg gaatcgtcgc cgaacccgaa gtcggcgaaa tctacgaagg caaggtcgtc
     2761 aagatcatgg atttcggcgc cttcgtgaat ttcttcggac cgcgtgacgg tctggtgcac
     2821 atttccgaac tggcgtccaa acgcgttgcc aaggtcaacg atgtcgtcaa ggaaggcgac
     2881 acggtgaaag tgaagctcat cggcgtggac gaccgcggca aggtgcgtct ctccatgaag
     2941 gtggtggatc aggcgaccgg cgaggatctg tccaaaaaac agggggccga gggcgagcag
     3001 caggccggct gatcgttttc aggccctcgt agaaaacaac gcccgggaga ggcatgtttc
     3061 ccgggcgttt gcgtggctgc catgcggttc aggataggct acattgaagc gctatgatcg
     3121 ttttccggct cttcgggctc ctgctcatcg ttctggcgct gatgctgctc ggcgcggatc
     3181 tggtgagcac gcttgaaaag aacggtgaaa ccgtgatccg gtctctcgat cagattttga
     3241 tgctcctcgg tgccgacgtg aagccttggc ttcaggagca actttcaccg caattcgcca
     3301 atgttttgct tgtcgctctg tcatggccgg gctgggcgat cctgggcatt ctgggcacgc
     3361 tgcttgccat gctgtcggcc ggtcaccgcg aaccccgcgc gccccttccc ccgcagccgc
     3421 cagcgaatcc catcaatcga taggcaacat caatcgatag ccgccatcaa tcgatctata
     3481 gtcgatgatg ttgcggcgtg cggttcgccc gctatgatcc gcagcccttt tgacaatatt
     3541 tggaaaaagc ccatgagcat cgaagccccg gaaacgattg tcgtcgatca cacgcgcgtg
     3601 gcctgcgagg gctccggcgg cgcgctcggt catccccgcg tctatctcga aatgggcgag
     3661 agggacttcg tcgaatgcgg ctattgcgac cgccgcttcg cgctcagtcc cgaagcgctc
     3721 gaagcacgcg aaaaaggtga ggcaggcgcg cattgaatgc acccgatcac aatgtactgg
     3781 gactgcgcgc gatttcaccg ggcgatcatc tctatctgat cgacgggtcg ggctttatct
     3841 tccgcgccta tcacgcgctg cctccgctca accgcaaatc ggacggactg cccacgggtg
     3901 ctgtgtccgg cttctgcaac atgctgtgga agcttctgga agacagcaaa gccggcgaca
     3961 agccaacgca tctggccgtc atcttcgatg ccggacgcaa aagcttccgt aacgatatgt
     4021 acacggacta taaggcgaac cgctccgcgc cgccggacga cctcgtgccg caatttccgc
     4081 ttacgcgcga tgccgtgcgc gccttcggaa ttgcctgcgt ggaaaaggcg ggcttcgagg
     4141 ccgacgatct gatcgcgacc tatgcgcgcc ttgcgcgcga ggccgaggcc cgagtcacca
     4201 tcgtttcgtc cgacaaggac ctgatgcaat tgatctatga cggcaaggtc gagatgctgg
     4261 atccggtgaa gaaccgccgc atcgggccgg ccgaggtgat cgagaaattt ggcgtggctc
     4321 cggacaaagt catcgacgta caggcgttga tcggcgattc catcgacaac gtgcccggtg
     4381 tccccggaat aggcgtgaag acggcggcta agctgatcgc ggaattcggc gatctcgaaa
     4441 ccctgctcgc acgcgccgga gagatcaaac agaacaaatg ccgcgaggcg ctgatcgaac
     4501 atatcgaaca agcgcgtctg tccgcacgat tggtgagact ggatgaagcc gttcccctcg
     4561 atgaggagat cgaccaattc gcggtgcgcg atcccgatcc ggcgaagctg atgccgtttc
     4621 tgaaagccat ggagttctcc aatctcctgc ggcgcgcggc ggcgaagctt ggcgtcgaag
     4681 atgccgaagc ggtttccgtg acgccgagcg tttccacagt cccgcaaccc cagccccagc
     4741 caagcccgcc gctcgccgat gccaaaatct gcccgggcgc ggtaatggat cgcgccgcgc
     4801 tccttaaacc catcgagtac ggcggatatg aatgcgtcac ggaaattgcc gcactcgacc
     4861 gctgggtggc ggctgcccat gaagcgggcc atctcgccat agcgttggaa tccgattcga
     4921 tcgatcccat gcgggcgcgg cttttcggcc tggcgctcgc aaccgcgcca ggcagggctt
     4981 gctatatccc gctggcccat cgcagcggcg aaggtctcgc ccttggcggc gcaacgcctg
     5041 cgcagttgac ggaagaagac gtattcgcgc ggctggggcc gctcctcgag aacgagagcg
     5101 tgctgaagat cggacacaat ctgaaattcg ccgtcgtgct gttggggcag cgtgggctag
     5161 cgatgcgcgc gctcgacgac accttgttgc tctcctatgt gctcgacatc gggctgcacg
     5221 gccacgggct ggaggagctt tcggaaaccc acttgcagca caggccgatc gcgctttccg
     5281 acattgccgg caaggggcgc gacaagatct ccttcgaaca ggtggcgatt gccgaggcga
     5341 cgcgttatgg agccgaacgc gccgacatcg cgctccggct tcatgccctg ctgaagccgc
     5401 gtctggtcgc cgagcggcgc atgaccgttt atgaaacgct cgagcggccg ctgatcggcg
     5461 tgctggccga gatggagcgc accggcatcg ccatcgcgcc gtccttcctc gccaagctct
     5521 cgaatgattt cgcgcgtgag atggcgcgac tcgaagaaga atgctgcggg cacgccggcg
     5581 agagattcaa tctcggatcg cccaagcaat tgggcgacat cctgttcggc aaattctcgc
     5641 tgccgggcgg caggcggacc aagaccggcg cctggtcgac ggatgccgac attctcgaag
     5701 aactcgccgc gcagggccat gcgcttccca aaacgattct gcaatggcgc cagctctcaa
     5761 agctcaaatc gacctacacc gatgcgcttc ccacctatgt caatgccacg acggggcgcg
     5821 tgcatacgtc ctatgcgctg ggcgccacca ccacgggccg gctgtcctcg aacgagccca
     5881 atctgcagaa catccccatc cgcaccgagg aaggacgccg tatccgccgt gccttcgtgg
     5941 cgcccgaagg gcgctgtctc gtctcggccg attattccca gatcgagctt cgcctcctgg
     6001 cgcacatcgc cgacgtgccg aatttgcgcc aagctttcgc ggacggcctc gacatccacg
     6061 ccatgaccgc gtccgaaatg ttcggtgtgc cggtgaagga catgccggcc gaaatccgcc
     6121 gccgtgcgaa ggcgatcaat ttcggcatca tctacggcat ttcgggtttt ggcctcgcca
     6181 atcagctcgg catcccgcgc ggcgaagccg atgcctatat caagaaatat ttcgagcgct
     6241 tccccggcat ccgcgactac atggaatcga ccaaggcttt cgtgcgcgag cacggctatg
     6301 tggaaaccat cttcggccgc cgcattcaca ttcgcgatgt gaaagccgcg agcgctgcca
     6361 cccgcgcctt tggcgaacgt caggccatca acgcgcctat ccagggttcg gccgccgaca
     6421 tcatccgccg cgccatggtg cgtctgccgc ccgcgctggc cgagaacgat ctcgacgcgc
     6481 atctcctgct gcaagtccat gacgagctgg ttctggaagt cgccgattcg caaggctccc
     6541 aggtttgcga tgtcgcgcgc gccgtaatgg agaaagcgcc gctgccggtg tttcaactgc
     6601 gcgtgccgct ggtggtggat gcgcgtgccg gcaagaattg ggacgaggcg cactgatgcg
     6661 attgataaag cttggcgccg ctacgcggcg agcgccctga gggcgtcttc gggcgcgatg
     6721 ccgatgcttt cgagctgggc ggcaagctca cccatggcct gatccttgtc gacgcccgtc
     6781 cggcgttcgt gttcaagctg cgcgatgtag gttgcgagga accccggctt cgccagcgag
     6841 gccatcagga gccgccgcgc tttcatcatg agatcgccct gcgtgaagat gccggccgcg
     6901 caaagtttga gcattgtttg cgcccgctcg atcggatttg cgatccgagt ctcgatggag
     6961 gcgagaaatt tcgcgcgcac ttcgatgcgt tgggccaccg tgtcgagggc catcgcgatc
     7021 tggttcttct ggacttcctg gaatccggaa cgcagcacac gttcctggat ctccgcgagg
     7081 cggcgcagcc gctgcgcaac cggtgctccg gcgaggagca gctcctcgaa attgttggag
     7141 gtgatgatcg gcatcatgaa ggccgagagc atgcgcttgt tctcggcgcc gacgatgttt
     7201 tcctcgacca ccagcagccg ttccagcttc tcgtccgagg atttcgaggc ctgcatgaat
     7261 tgctgcagcg gttcctggct gaccagtcgc cgtgaacgct cggtgaaggc gccgatcagg
     7321 tcctgattgg agagatattt gccctgcccg cggaccagcc gattgccgag ccgccgcagc
     7381 attttgaact cttcgtccaa ggaactcggg cagaggcgct tcaagccctt gagttcggcc
     7441 agaatccgtc cggcgattgc cgcgcgcgct tcgggaagct cgtccttggc aaaagatcgc
     7501 gccaaatcat tgatgccgtg ccctgcccct tcggccgcgt tgacgagggt tccgagaaac
     7561 agctcgacca ggttcatcag ggcatgtcca agatcgggat tgtgcccgag caggtccgcc
     7621 aaagccgctg cgccattcag catctcggcc accagcgtgt cgatggcggt caggagcagc
     7681 gcgcgtcccg ggccctccac cggcaactcc tccatcaatg tcaggagccg ttgcagcttg
     7741 gtgtcccagg tcttggcgtg tccgagatat ttcgcgagcg ccccattcag gatgtagcgt
     7801 ccttccggat tcgcgcccag cttgctggca agcggtccga attgtccggc ctgcagttcc
     7861 ggaaagagat tgcgtttctc gtccttgtag acgcgatgga tggcggccgt gcagagttcg
     7921 ttcagttgct tgacgatctg actgacggtg gtttcggatt ccgcggcctg tgccaccgcc
     7981 actttctgga tggcatgctg gtaagtggtg ccggtggcct cgaacctttc cagtgcggcc
     8041 gcgccgtgga tgagttcggt caccgtcagc ttctggcgtc ccagccattc gccgaggacg
     8101 cgcgtcatgg tcatgcgcgc gtgataggaa tagagatcgt ccggcttgaa gcagggcgag
     8161 aaggtggcga tgtcctcgac cttcttgttt ttctttttga cctcggtatg gccgccttcg
     8221 aatatcgtga ggctcatata atcgccggtc gaggcctgat acgtctcctt caccacgcgc
     8281 acggcggcgg cctggccttc ttccagcatc tggcggccac gctcgagcgc ggcttcgcga
     8341 tcttccatcg cttcggccag ggaccagcca ccgcttttgc cctgttggcg aaaaatctcg
     8401 aaatggactt gggacataag ctcaccggcc ggctgccggg aaagaattaa cggctcggtc
     8461 ttaaatgccg gttaagcacg cttccgccgg tgcggcatgt gtggacaggt cgcggatggt
     8521 gggtgccgaa ccgcaaaggg cacaccccgg gtctttgggc actttaaggg tgcgggattg
     8581 tgccgccagc gcgtcataga ccagaagccg ccctgccatg gtttcgccga tggcaagaat
     8641 ttccttcatt acctcgagcg cttgcagcgt tcccataatt cccgcaacta caccgagcac
     8701 acctgcctcg ctgcaacttg tcgtcgtctc aggcggtggg ggttcggcaa acaggcagcg
     8761 atagcagggg taaaggccgc ttccgtccca ggccttgtag gtcgcgagct ggccgtcgaa
     8821 ttcggtgacg gcggcggaaa ccagcgtttt cttcgcgaaa tagcaggcat cgttgatgag
     8881 gaaacgggtg gcgaaattgt ccgatccatc ggccacgacg tcgtagccgg cgatcagatc
     8941 ggccgcattg gccgccgtca ggcgcatcgc atgggggacc attttcacgc cgggattgat
     9001 ggcgtgggcc gcgtcgcggg cggattcggt tttcagacgc cccacatcct gggtgcgatg
     9061 ggcgatctgg cgctgcaggt tggaaaggct cacccggtcg aaatcggcga tcccgatggt
     9121 ccccacgccc gccgccgcga gatagaggat cagggggctg cccagccccc ctgccccaat
     9181 gaccaggacc tttgcggcca gaagctttgc ctggcccgcg ccgccgactt cgcgcaggac
     9241 gatgtgccgg gcatagcgtt cgagttcttc gtcgtcgagt gggggccggg agcggggtat
     9301 ttcgttcatg gcccttcttt agcatggggg ccagtcttta gcaggcagcc atctttagca
     9361 ggcggcagtg tttggcaggc aagaggcgag cgtcgaaaac ggaaaatcca tgggggcaag
     9421 cggttaacta gggtggcacc ccgcgattgc ggcactttgc ccatcgatct atcttgcggg
     9481 gagacccggg ttggaccgat aaagcggcgg ctcgggtaag gctcttcaat agggtcgtcg
     9541 caccgggaga acaagcggga gacgctcgca acctgtcatc ggcaaagctc tgccgggtag
     9601 ggttcgccgt cgaacaggcg ggcgggactg ctttcgtgat ggtgcctgcg caaggcaagg
     9661 ccggaatccg gatgcgatcc ttgagaaggt catagccggc aaaggcaata aagaggtttg
     9721 agaatgaccg taccaatggc tgtcccgaca accccgacac gcaacaaaaa gcttctcgcc
     9781 tgggtggagg aggtggccgc caaatgcacg cccgaccgga tttattggtg cgacggttcg
     9841 caggccgaat atgatcgtct ttgcgccgag atggtgcagg ccggcacttt catcaaactc
     9901 aacgaaaaga agcgtcccaa ttcctttctc tgccgctccg atccggccga tgtggcgcgc
     9961 gtcgaagacc ggaccttcat ctgctccacc gacaagacag acgcgggtcc caccaacaat
    10021 tggatgaacc ccaaggacat gcgcgcgacg ctcgatcgcc tgttcacggg cgcgatgcgc
    10081 ggacgcacca tgtacgtcgt tcccttcagc atgggcccgc ttggctcgcc gatttcccat
    10141 atcggcgtgc agctgacgga cacgccctat gtcgcggtca gcatgcgcat catgacgcgc
    10201 atgggccagc aggcgctgga tgtccttggc gacggcgatt tcattccctg cctgcattcg
    10261 gtgggcgcgc cgctggcgcc gggtcagaag gacgttgcct ggccgtgcaa cgccgaacac
    10321 aaatacatcg tgcatttccc cgacgattat tcgatccact cctatggctc gggctatggc
    10381 ggcaatgcgc tgctcgggaa gaaatgcttc tccttgcgca tcgcttccct gatgggccgc
    10441 gatcagggct ggctcgcgga gcacatgctc atcctgggcg tggaaagccc gcagggcgaa
    10501 aagacctatg tcgcggcggc cttcccttcc gcctgcggca agaccaattt ctcgatgctg
    10561 gtgccgccaa acggcttcga tggctggaag gtttcgacca tcggcgacga catcgcctgg
    10621 atcaagccgc gccccgatgg cacgttccgc gccatcaatc cggaagcggg cttcttcggc
    10681 gtggcgccgg gcaccgggat gaaatccaat cccaacgccg tgaagatgct caacagggat
    10741 gtgatcttca ccaatgtcgc gctgaccgac gatggcgatg tgtggtggga agggctgacc
    10801 gatgagcccc cggcgcatct gatcgactgg cagggcaagg attggacgcc aaactcgggc
    10861 cgcaaggcgg cgcatcccaa ttcgcgcttc accgcgtccg cgcgccaatg cccgagcttc
    10921 gacccggcgg gcgatgatcc cgaaggcgtg ccgatctcgg cgttcatttt cggcggccgc
    10981 ctgtccaaga ccttcccgct cgtctacgaa gcgcgcaact ggcagaacgg tgtctattgg
    11041 gccgccacca tgggctccga agccaccgcc gccgcggtca accaggcggc gatccggcgc
    11101 gatcccttcg cgatgctgcc cttctgcggc tacaacatgg gcgattattg gaagcactgg
    11161 ctcgccatcc aggacaaggc aaagaagctt ccgcgcatct atcgcgtcaa ttggttccgc
    11221 agggacgata acggcaaatt cgtctggccc ggctttggcg agaacatgcg cgtgctcaaa
    11281 tggatcgtgg atcgcgccaa tggccgcgcg aaggattcgc cggaaggtcc attcggcttc
    11341 atgccgcgct accaggatct gcactggacg ggcatggaat tttccaaaga caaattccac
    11401 cgcatcaccg acatcgatcg cgccgaaggg ctggaagaag ccgaagagca gtccgagcat
    11461 ttcggaaggt tcggcgaccg cctgccgacg caactcgaag ccgaacgtca ggacctcatc
    11521 cgccgcctga aggccgcgcc gccggtgtgg accacaggct agtatttccg ccctactaga
    11581 aatacttagg ccttcaagga tttatcgcgc agcatgcgcg cagcatgaaa ttcatgctgc
    11641 gcagcatagc tgcggcatga ccggagcaaa ttcgctctgc gcgagccggt gttcgcgccg
    11701 ctttcgctta gataaatcaa aagcaatctc gcttcccagg aacgccattc gaacggcccc
    11761 gttaccgtat cactggggct ggtttccacg tttcatggag gaagcaatgg ctcttaagaa
    11821 cttcactctc acagccgccg ccctgattgc gctcacaagc gcaagcttcg cggcaaacac
    11881 cacgacaggc acgtctgctt caaaggcgac caccgaagtc gaaaaggccg tcggggccgg
    11941 cgatacgctg tcggctacgg atctgaaatc cgcggtcaaa ctcggcacgc tcaaagatgc
    12001 taaggacaag ctcgccacgg cgaaagtcga cgatccgatg ggcaacatca tcggtcccgt
    12061 gaaggatgtc gtcactggca aaacgggtgc gccgaccgcc attcacgtcg atgtcggcgg
    12121 ttggctcggt gttggcgaac gcgtcgtgtc gatcaacgcc gggaatttca cctatatccc
    12181 cagccgcgat attctgctga ccaagatgag caaggcggac atccagaagc tgccggcggt
    12241 caaacagaaa agctgaccca ttgcctacga acgcgagcac gcgggcatcg gcctgtgtgc
    12301 tcgtttttgc gggcggcatt tcggcgcggc cgcgtcctgc gagtgctatg aagcggtcga
    12361 aagccatttc gccagattgc tgccgaaaga cgcggatgcg gcgaagggct aatcggcgcc
    12421 gccgctcagg aattccttca tcttcgcgaa gaagctttcc gaatccggat tgcacccttg
    12481 cggcgtttca gtttcgaaac tgcgcagcaa ttccttctgc ttcctggtga gcttcaccgg
    12541 ggtctcgaca cggacttcga cgaagagatc gccgtgatgg ccgcccgatt tcagcacggg
    12601 catgcccttg ccgcgcagct tgaactgatg tccggtctgc gcgccttcag ggatcgcgat
    12661 gcgcgctccc gcgcccgaaa gtgtaggcac ttcgatctcg ccgccgagtg ccgcggtcac
    12721 aaacccgatc ggcacacggc aatagagatc gtgcgcgtcg cgctggaaga tcggatgctg
    12781 ctgaaccgag aggaagatat aaagatcgcc cggcggcccg ccgttcaggc ccgcttggcc
    12841 ttcgcccgaa aggcggatgc gcgtgccctc ctcgaccccc ggcggaatgt ccaccgacaa
    12901 ggtgcgctct ttctgcacat gaccgtgacc gccgcaggct ttgcacggat gcttgaccac
    12961 gcggcccgcg ccccggcatt tggggcaggt gcgctcgatg gtgaagaagc cctgagtcgc
    13021 gcgcaccttg ccgtggcccg cgcaggtggc gcaggtttcc gggctcgtgc cgggcttggc
    13081 gccggtgccg gtgcattcct cgcaggcgat gagcgagggc acgcgaatct ccgccgcctt
    13141 gccgcgatag gcatcatcga gcgtgatttc catattgtag cggagatcgc cgccgcgatt
    13201 ggagcgccgg ttgcgcttgc cgcccatgaa ttcgccgaac agatcgtcga acacatcggt
    13261 gaaggaggcg gagaaatcga acccgccggg gcccccgcgt ccgccatttt cgaaggcggc
    13321 gtgaccgaac cggtcatagg cgccgcgctt gtcgggatct ttcaggacct cataggcctc
    13381 gctcagttcc ttgaacttgt gctcggccgt atgatcgccg ggattgcggt ccgggtgaca
    13441 ttccttggcg agcttgcgat aggcaccctt gacctcttcg gccgcacagg tgcgcgcaac
    13501 gcccaagatt tcgtaatagc agcgtttgct cattccattc ctccgcgcga cgaggacaag
    13561 cccggagcat cacgccccga gcccgccccg cggaggcctt catgctcgga gaagctcagc
    13621 atgaaggtcc tttaattctc gtttccttga gcatccgcgc cccccgacgc cgagccgtgc
    13681 gcaatcacgc cgcgcctttt ttctgatcgt cgacctcgga gaattccgca tcgacgacat
    13741 tctcatcctt gggagcgccg tcgccgtcag gaccggcacc cggtccctcg gcgccatcgc
    13801 cgccgccgct ttgggccttg tacatggctt cgcccagctt catcgaggct tgcgccagcg
    13861 cattggtctt ggccttgatg tcctcggcat tatcttcctt gatcgcggcc ttcagggctg
    13921 cgatcgcatt ttcgatggcg ctgcgttcgg cgtccgacac tttcgagcca tgctcggcgg
    13981 ccgctttttc cgtggaatgg atcagcgcat cgccgtgatt cttggcctcc gcaagctcgc
    14041 gcgtctgctt gtcgtccgcc gcatgggctt cggcttcctt gaccatcttg tcgatgtcgg
    14101 cttgagaaag tccgcccgag gcctggatgc ggatggtctg ctccttgccg gtggccttgt
    14161 ccttggcgcc caccgaaacg atgccattcg cgtcgatgtc gaaggtgact tcgacctgcg
    14221 gcactccgcg cggggccggt ggaagaccga ccaggtcgaa ctggccgagc agcttgttgt
    14281 tcgccgccat ctcgcgttcg ccctggaaga cccggatcgt gacagccgtc tgattgtcgt
    14341 cggcggtcga gaagatctgg cttttcttgg tcggaatggt ggtgttgcgc tcgatcaggc
    14401 gggtgaacac gccgccgagc gtttcgatgc cgagcgaaag cggtgtcacg tcaagcagca
    14461 ggacgtcctt gacctcgccc ttcagcacgc cggcctggat ggccgcgccg atggcgacca
    14521 cttcatccgg gttgacgccc ttatggggct ccttgccgaa aagctgcttc acggcatcct
    14581 gcaccttggg catgcgtgtc atgccgccga ccaagatcac ttcgtcgatc tgattggcgg
    14641 agacgcccgc gtccttcagc gcctgcttca ccggcgcgat ggtcttctgg atcaaatcgt
    14701 cgaccagggc ttccagcttg gcgcgcgtga tcttcatggt caggtgcttg ggccccgacg
    14761 catcggcggt gatgaagggc aggttcactt cggtctgcat cgcggaggac agctcgattt
    14821 tcgccttttc ggcggcttcc ttcagccgct gcagggcaag gcggtccgag cgcaggtcga
    14881 tgccggattc tttcttgaat tcgtcggcca ggtaattgac cagccggatg tcgaaatcct
    14941 cgccgccgag gaaggtgtcg ccattggtgg acttcacctc gaacacgccg tcgccgattt
    15001 ccaggacgga gatatcgaag gtgccgccgc ccagatcgta gaccgcgatg gtgccggcgt
    15061 tcttcttgtc catgccatag gcgagcgcgg ccgcggtcgg ttcgttgatg atacgcagaa
    15121 cttcgagccc ggcgatcttg ccggcgtcct tcgtcgcctg acgctgggcg tcgttgaaat
    15181 aggcgggaac cgtgatgacg gcctgactga ccttttcgcc gagataggat tcggccgttt
    15241 ccttcatctt ctgcaggatg aaggcggaaa tctccgatgg cgcgtattgc ttgctctcgc
    15301 ggccctgcac ccaggcatcg ccattgccgg ccttgacgat cttataaggc accatgccga
    15361 tgtccttctg ggtcatcggg tcgtcgaaac gccggccgat caggcgcttg atggcaaaga
    15421 acgtatgctc ggggttggtc acagcctgac gcttggccgg ctggccgatc agccgctcgc
    15481 cctcgggcgt gaacgcgacg atcgagggcg tcgtccggcc cccttcggaa ttctcgatga
    15541 cttttggcgc cgatccttcc atgacggcga cacaggaatt ggtcgtgccg agatcgatac
    15601 cgataagttt tgccatatat aatcctcctg atggcggatg aacatttcgc agcccttcaa
    15661 ccgggaagcg gctgccaatc catccccttc gatgcaaagc caaaaccaaa gacgctacgg
    15721 cggtatatag ggaggccgtc ctgatgccgc aaccggatga cgcaccgttt ggctgcggtt
    15781 tttagggccg gcgcgggaaa accggggacg aagacctcat ggagccgggc ccggtccatt
    15841 tgtccaagag agcggctaag ctcttgtata aggtgatttt ttcatgcggc ccgttcagta
    15901 gggatcgtaa tgcgccaatt tggggtcgtg gaaggccgtg agcgcgtcgt gatagtcgtg
    15961 ttccaagcgg ccctcgacat attcgcggct ataggcttcg cggcacgttg cgaattgggg
    16021 atccttttcg gtgatgccta cggcattgca attggcgatg gccagcctgt cggcccgatt
    16081 gcggtctgtg gcgcagccgc aaagcgccaa accaaccgat gccatgagga tgagataacg
    16141 catgaccggc tccctccagc cgccgctctg gcggcggcca tttttcgaca tcgtaccata
    16201 agccgggcca tgcctcattg gcaaaacggc gcgccgcggg cacaccctgg cacgccctag
    16261 taaacatcct gatgcggaat gcggtaatcc agctcattgg gtacgcgctt aagggcgtcg
    16321 cggtaacttc tgtcgagctg gttttcgagg tgctggcgcg aataggccgc cgagcaggtc
    16381 gcaaattgcg gatccttctc gctgattccg accgcctggc attcgagatt ggcctggcga
    16441 tagacgcttt catcggtggc gcagccgcaa agccccaaaa taccgatgat cccggcaatg
    16501 ctgacgcgca tggcgattcc tgcagcgcag agccttccgt ccagaagaat agcataaacc
    16561 gcggaaaata cgtccgcagg gtccgccgcc gattttcgcg atgccgagac cggcgcgcta
    16621 tccgccggat ggctgcgccc tggcgtcgtc ccgcgcggcc tccagatcgt cctgccgcgg
    16681 cgtggcctgt tgttgcgcga ccggggccgg cattatggcg atgaaacgaa ccggatgcgc
    16741 gcccggtacg gcgagcgtgg cgggcagaag tgccagcatc atgacaaggc ccggtccggc
    16801 gtgaagatat tggcgatgat tgagcaaggt gacgacgccg ccgaaattga tggcggccgc
    16861 gagcccgatt ccccagaggc gaaacacagg gtcggcaagc caagccgccg cgataatttc
    16921 cagaaagccg acgatccgcc gtaaattctg cgaatattcc caacgctcat aggcctcgcg
    16981 cacgaagcgc ggaccggcca gatggaccaa accgattgcg ccgaagagag cggcaaggat
    17041 caaagcaacg taattcgaga tgacgtgcac gcaaaaactc caaggatgga ccgcggcaaa
    17101 ggctgcgatt tgcgcggttc acatgaggat cggaaatttg gcgttcaatt ggtgtaagtt
    17161 ttcgacgaaa tggctggccg cctcttaagg caaaggtctt gaagacgtgc aaagccgaaa
    17221 gcgcgatttt tatcggtttt tgaaagaaat ccgagagtgt tcccgcagcg tctcatgcgg
    17281 acgggacagg gtgcgatgat cacacaaggc ctgatgatcc cggacagcta tgcgcggctc
    17341 gcatccatcg ccaaatgccg aacggagctt caggcgttgt tccgtttttg ggagaagctg
    17401 cgcgggccgc gcagcatgcc ggcgcggcgc gatttcgatc cggccgattt gcgcgcgctg
    17461 ttgccccatc tcatcctgat cgatgtcttc gcgcagaagc cgcccgagcg gcgtttccgg
    17521 gtccgtcttc aggggaccgc tcaggtcgat taccagggaa gcgactggac cggctgttat
    17581 ctgcatgaaa agacggagca ggcgtcagcc gaccggcttt gcgccgtggg cgatcatatc
    17641 gtcgccaccc atgagccatg gatgtcgacc ggcgggctct attggacgcg ccgcaaaccc
    17701 tattcgcgtt ttgaatccat cctgctgccg ctttcggatg atgacgccac ggtcaacatg
    17761 attctgggcc tgacgatatt ttctgcctga ctttccccgg catccgcggg gaaaggaagc
    17821 ttaagtcgcc cttggcccca tggaaaccgt cagaaatctt tacactttcc gttaatttaa
    17881 caaaggcgcg taatgtaact agcgggaaag gctcatttaa agattctggc acaattgttg
    17941 ctatttgcgg cgggtgcttg agtaatcatt ggaaaaaagc agggggacat gtcgatgagg
    18001 accgtcaaat cggcccgcgt agctgtgggc gtggctttgg ccgccagttt ggggctgttt
    18061 tcgggttctg cttcggccac accaataacc gttgccaatt tttcgtttga aaccccgggc
    18121 accaacaatg gcggcgcagg ctgccccatc accggttgga cgtgtagcac caacagttcc
    18181 agcatgggtg tgtatgttcc ggttacgacc caattcccct ctggcgccaa tgggctcagc
    18241 ggaggcgcca ttgtgccgga tggcactcag gccgcttatc ttgccctcac caatggcttc
    18301 atcagccaaa acaccgctgc ccttatcgcg aatagcacca cctacacgct gagtgtgttc
    18361 gtcggccgcc gcaacgattt caatcctgcg atttcggggt cgattgcctt gacaaacaat
    18421 ggcactgtgt tcgcgaccct tgcgatgtcg gcgcccgcgc aaggccagtg gctcgatgag
    18481 actgtctcct atacgacgac gcttgcggac agtctgcttt tcggtggcac gctcggcatc
    18541 agaatcaccg atctcaacgg caatcaattg aatgtcgacg acgtaagatt gagtcagtcg
    18601 accacgacgg tcaccgatgt tccggagccg gcaacactgg gtcttttcgg gctgggcttg
    18661 gcagcggtcg gagtggcgcg gcgcaaacgg cgtgccgctt aacgcgaagc acgcgccgca
    18721 caaaaacgat cacccgccaa ggcgctttgc cagacgcgcg cgataggcct ccatatcgag
    18781 aatcggccgc cgcgccacgc ctgttctcat ggcggcttcg gcgaccgctg acgagacccg
    18841 agaaatcagc cgtggatcga acggtaccgg aatgatgtag tcccgcccat aggcgtggcg
    18901 cgccccctga taggccgccg ctacctcatc gggcacagtc tcgcgcgcaa gttcggccag
    18961 tgcgttcgcc gccgcgatct tcatttcttc attgatggtg gtggccgcga catcgagcgc
    19021 accgcggaag atatagggaa agcccagcac attgttgatc tgattcgggt agtcagaacg
    19081 cccggtggcg atgatggcgt cgccgcggac ctgacgcacc tcttcgggcg tgatt
//
LOCUS       DASYEG010000124         2189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4242770, whole genome shotgun sequence.
ACCESSION   DASYEG010000124 DASYEG010000000
VERSION     DASYEG010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4242770"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(275..350)
                     /locus_tag="VGM72_05245"
     tRNA            complement(275..350)
                     /locus_tag="VGM72_05245"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(315..317),aa:Val,seq:tac)
     gene            complement(404..583)
                     /locus_tag="VGM72_05250"
     CDS             complement(404..583)
                     /locus_tag="VGM72_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264702.1"
                     /translation="MTAAPPNRRAFALRVQPDVLAAVERLAAAELRSTNAQIEILLRE
                     ALARRGARRRPVPGD"
     gene            complement(580..1437)
                     /locus_tag="VGM72_05255"
     CDS             complement(580..1437)
                     /locus_tag="VGM72_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY4264703.1"
                     /translation="MEHSVERPGAGFSGGLALIVCLLLLAGAIASILAGAQGAVSGPV
                     FAIVFVMTVAGFYSLQPNEAAVLTLFGRYAGTDRQNGLRWRPFWYARKKISLRARNVT
                     SETLKVNDKRGNPVETAANVVWHVSDTAQAFFDVDNYETFVHIQIETGLRATASRYAY
                     DHGEEAEPTLRDGEIISEVLRTELQERVHAAGIGIDDARISHLAYAPEIAGAMLRRQQ
                     AQAILAARRLIVEGAVGMVEHALKQIGERGLVALDEERKASMVSNLLVVLCSDHDAQP
                     VLNTGTLYN"
     gene            complement(1593..1901)
                     /locus_tag="VGM72_05260"
     CDS             complement(1593..1901)
                     /locus_tag="VGM72_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY4264704.1"
                     /translation="MRESPAQRRTRVNKNDLVQKLAERTGLSKMDAQKAVDGVFDVIT
                     DSLKGGDEVRVTGFGVFVVSERAGGKGRNPQTGEEITIKASKAARFRAGKPLKDALNA
                     "
BASE COUNT          429 a          708 c          643 g          409 t
ORIGIN      
        1 ccgaacgcgc aggaagaccg cgacgcggcc cgccgcacgc cgaggcaaga aagatcacgg
       61 cagcgctcat tgcgaatatc cgaatcgcgc gcgtcatcgc agaggccaat cgaacgtcct
      121 cttcttttag cgcgccctgg cagaatggcg catgaattcg gtgcaagccc atatccatac
      181 ccgcaaactt agccgaaact cgccaggctt gccgagccgt agcattacgt gctcgctcgc
      241 ttcggcgcgg caaccttgct cgatccgcga aaattggtgg gcggtgacgg gctcgaaccg
      301 ccgaccctat cggtgtaaac gatatgctct accaactgag ctaaccgccc gtgacctgcg
      361 ccttacagca ccggcggtgg cgtttcaatc ttatgaagcg cgttcagtcc ccgggaacgg
      421 gccggcgccg ggccccccgc ctggccaggg cttcgcgcaa aaggatttcg atctgggcat
      481 tggtgctgcg aagctcggcg gcagccaggc gctcgaccgc cgccaggacg tccggctgga
      541 cgcgcagcgc gaaggcgcgc ctgttcgggg gtgcggcggt caattgtaca gcgtacccgt
      601 attcaggacg ggttgcgcat cgtggtccga gcacaacacg acgaggagat tggagaccat
      661 cgaggctttg cgttcctcat cgagcgcaac gaggccgcgc tcgccgatct gcttgagcgc
      721 gtgctcgacc atgcccacgg ccccctccac gatcagcctg cgcgccgcca gaatggcctg
      781 cgcctgctgg cggcgcagca tggcaccggc gatttccggc gcgtaggcaa gatgggatat
      841 ccgtgcatcg tcgatgccga tgcccgcggc atgcacgcgt tcctgcaact cggtccgcag
      901 cacttcggag atgatctcgc cgtcacgcag cgtgggttcc gcctcttcgc catgatcgta
      961 ggcatagcgc gacgcggtgg cacgaaggcc ggtttcgatc tggatgtgga cgaaggtctc
     1021 gtaattgtcg acgtcgaaga atgcctgggc ggtatcggag acatgccaca ccacgttggc
     1081 ggcggtctcc accgggttgc cgcgcttgtc attcaccttc agcgtttcgc tggtgacgtt
     1141 gcgcgcgcgc agcgatattt ttttgcgcgc ataccagaac ggccgccagc gaaggccgtt
     1201 ctgccggtcg gtaccggcat agcgcccgaa caaggtcagc accgcggctt cattgggctg
     1261 cagcgaataa aagcccgcga ccgtcatcac gaagacgatc gcgaacacag gacccgagac
     1321 ggcgccctgc gccccggcga gaatggaagc aatcgcgccg gccagaagca gaagacacac
     1381 aatcagcgca agtccgccgg aaaaccccgc acccggacgc tcaacggaat gctccatgtt
     1441 tcgagtttcc accatcatcg ccatcctctt agatatcatc taagtatcat agtgatatca
     1501 caatgggaag cagcttcccg gcacgggcat aggcgcgcct aacgcagcac ccggcagcgc
     1561 ttcatcgaga gaggaaaggc aatcctgaaa cgttaggcgt tcaatgcatc tttcaacggc
     1621 ttgcccgcgc ggaaacgcgc ggcctttgac gccttgatgg tgatctcctc gcccgtctgg
     1681 gggttgcggc ctttgccacc cgcccgctca gacacgacaa agactccgaa ccccgtgaca
     1741 cggacctcgt ctcccccctt gagagagtcg gtgatcacat cgaacacgcc gtcgacggcc
     1801 ttctgtgcgt ccattttgga aagtccagtg cgctcggcca gcttttggac aagatcattt
     1861 ttgttcacgc gcgttctcct ttgggcggga ctctcccgca ctcgccaaga ttaaggccgc
     1921 tttttaggcg ccgtcaaagc ttgaatcctc gaaaaaacac ccccgcgggg attgccccgc
     1981 gagggtattg gcatgattcg ctccgagaaa accgcgtcag tgggtcatga cagcgtcttc
     2041 ggacccatcg tccgcccgtc tggcttgagg cgggtcctcg ggctcctccc acaccaccgg
     2101 gatcggaaca cggaccagcg cgatcttgag cacctcttcc atgttggaga cgggaacgat
     2161 ttcaagttct ttcttcacat tatccggta
//
LOCUS       DASYEG010000125         7525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1196656, whole genome shotgun sequence.
ACCESSION   DASYEG010000125 DASYEG010000000
VERSION     DASYEG010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 7525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7525
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196656"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VGM72_05265"
     CDS             <1..679
                     /locus_tag="VGM72_05265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HEY4264705.1"
                     /translation="VTGALSSDALGEAALDAGLYDDAEVEIWRVNWATTSQRVLMRKG
                     SLGQVKRGKLGFEAEIRGLAQALNQPMGRAFGYSCDADLGDARCTVDLTEAALHSSGV
                     VIAATDARRFTVSGLSAFASGWFSGGKLLWTDGANAGRAMEVKRHAISASSVSIELWQ
                     AMSETLMAGDAFTVTAGCDKQFATCKAKFVNQINFRGFPYMPGNDVVLSYPSRGDALD
                     GKSRYGH"
     gene            669..1100
                     /locus_tag="VGM72_05270"
     CDS             669..1100
                     /locus_tag="VGM72_05270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264706.1"
                     /translation="MAIERGRIIAAARGWIGTPYVHQASLKGLGCDCLGLLRGVWREL
                     MGDEPEAPPPYSADWAEARGDETLYAALARHFAEIDRIDIAPGDIALFRMAASGPAKH
                     CGIVAELGGIPSLIHARQNKRVSEEELSRVLRRKLAFAFRV"
     gene            1104..5048
                     /locus_tag="VGM72_05275"
     CDS             1104..5048
                     /locus_tag="VGM72_05275"
                     /inference="COORDINATES: protein motif:HMM:NF024936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase TIM-barrel-like
                     domain-containing protein"
                     /protein_id="HEY4264707.1"
                     /translation="MATLILESAGQAVGALFGGSVGGIIGSALGALAGASIDNALFAT
                     HGTANREGARLSDVSIQASTEGAAIPVIYGRVRVAGEIIWATRFKETAVTTSQSAGGG
                     KGGGGGTTVVSTDYLYSISFAVGLCEGIVHQLGRVWADGKLFDLSKATTRFYPGTQDQ
                     GTDPLIEEIEGAGNTPAYRGLCHIVFEDLPLAEFGNRIPQLQFELIRSLSADNPDSLE
                     SRLGGVALIPGAGEFVYATDSVNADDGQGGTASQNVHGASGVADIVASLNDLETLAPN
                     LGAVSLVVGWFGDDLRAGHCTVKPGVEVASKTTYPQDWSVNGVARADAHLVSQVEGRP
                     AYGGTPSDASVVQAIAALRARGWRVMFCPFLFMDVPDGNTLSDPYTGAAGQPAYPWRG
                     RVVCDPAPGVAGSPDKTSAAATQVNAFFGSASAGDFSVSGTKVSWTGGTDWGWRRMVL
                     HYAHLCAAAGGADAFLIGSEFTALNHVRSSSTAYPAVAALKTLAADVRGILGEATRIG
                     YAADWSEYNNHQTGDAPGAVLFHLDALWADANIDFVGIDNYMKLSDWRDGTAHADYDA
                     VNGPTDIHDPAYLAANIAGGEDFDWYYASDADRLSQTRTSITDGVAGKPWVFRAKDLV
                     GWWSNAHYDRANGTEAGSPTAWVPQGKPIWFTEIGCPAIDKGANQPNVFFDPKSSESA
                     VPYFSNAGRDDLIQRRFLEAHLKYWSDTGNNPVSGVYTASMVDIANIYAWTWDARPFP
                     FFPARGDVWGDTANYRLGHWLNGRLGSVLLADLVADICRHTGFADFDTADLSGLVTGY
                     LIGETMSPRDQIEPLGLAFHFDAVESEGIIRFLTRGQIPVCSLTEDDLALPEDQASLG
                     ANFVRAQDADLPNVSRISYIDAGADYRESMAEAHRLTGGSNRVAQSALPLVLDQGEAI
                     GIGERLLQDAWVARETANFALPPARIALDPADEVTLAVGGRTRRLRVTEIGDAGPRAI
                     AAVMTDPSIYETLNGPDRGVGALTGVNVAGRAILAFLDLPLLTGTELAYAPHVAAYAT
                     PWPGSVLVYRSTSDANYALDTQLATAAIMGELRFDFYAGATGRWDKGNQLWLTLYGGT
                     LSCVPDIDVFAGTNTLAIENGDGEWEIVQFRDAELVGSGQWKLTTLLRGQAGTESAMR
                     NPVGAGARIVVLDRDLPQLHLSLDSRALPFFYRWGPNGKAISDATYQTAQRQFVGIGL
                     RPLSPVQIRARWPTVAGDIVISWKRRTRIGGDSWEQLDVPLGEDSENYEVDIFNGATV
                     VRTLQASMPQATYTFAQQTSDFGAQQWSVTVAIYQISAVFGRGAQRKATLFY"
     gene            5058..5579
                     /locus_tag="VGM72_05280"
     CDS             5058..5579
                     /locus_tag="VGM72_05280"
                     /inference="COORDINATES: protein motif:HMM:NF022436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2793 domain-containing protein"
                     /protein_id="HEY4264708.1"
                     /translation="MSDSTNLQLPYLDGAQAQKHVTVNESLRKLDALVLLAIEDKDLT
                     TPPGSPSDGARYIPKATAAGAWSGKENAVAHYVDGAWEFYPPREGFIAYLRDEDQLYL
                     YNGSAWIALSSSFATLSVSGAVRVGSFMVSELGSASPASQIVYVSNETGGAVIAFSDG
                     TNWRRVTDRAVVS"
     gene            5608..6012
                     /locus_tag="VGM72_05285"
     CDS             5608..6012
                     /locus_tag="VGM72_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264709.1"
                     /translation="MAHNVKYSTALKNSRLDQITSKIGASGLLRIYSGTQPANPDIAV
                     TSQVKLLEFTGDATAFAGAASGGVLTANAIADATALASGTASWFRLCKDDGTPAVDGT
                     VGISGTDCIIDNISITAGQSMPVISLTITSAN"
     gene            6028..6936
                     /locus_tag="VGM72_05290"
     CDS             6028..6936
                     /locus_tag="VGM72_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264710.1"
                     /translation="MTTSDVPVPGGTQRVPTYAVSEDSKTKDLERVALNESDGSDLAL
                     GAKLGALAETAPATDTGSSGLNGRLQRIAQRLSTLIANLAFVFGAGTASAAQRVTLAS
                     DDPAVATLGSTSGAKVIADANGTIQQYLRGLVAQWISGTLTLASRAASASVALTRTAD
                     TNAYAANDVIGAATGSTAAIQFSNMGPSGAVVQIVSIELEIDSTGVISGETSYRGYLY
                     NVTPPSALADNAAFDLPSGDRASYLGFLDFGTPVDLGSTLYVRATPDNCFVKLSGTDI
                     FMYLVTNGPYTPTSARVYKPKLFAVA"
     gene            6936..>7525
                     /locus_tag="VGM72_05295"
     CDS             6936..>7525
                     /locus_tag="VGM72_05295"
                     /inference="COORDINATES: protein motif:HMM:NF027224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HEY4264711.1"
                     /translation="MTADYFSSDGATYPNYDNIGPCAFYDAADNKTWDCREAWNGSHR
                     QITVTVFDHGAGNITDYTVSTNTLTNDDHGEGALCMDHEGYVHLYWGAHLSPLHHAVT
                     TNPRDPTAWTIRPNITTTHPGAGGVSYPHPCQVGSVQYLLLRDTLSGTHKPCCLFYTT
                     SLSGGVETWSSRIDIADYGLGSDGLGTWFYSSLMYVA"
BASE COUNT         1355 a         2336 c         2354 g         1480 t
ORIGIN      
        1 cgtcaccggc gcgctctcct ccgatgcgct tggcgaagcc gcgctggacg ccggtctcta
       61 tgacgatgcc gaggtggaaa tctggcgcgt gaattgggca acgacatccc agcgtgtgct
      121 gatgcgcaaa ggttcgctcg gtcaggtcaa gcgcggcaaa ctcggtttcg aagcggagat
      181 acgcggcctc gcgcaggcac tcaatcagcc aatgggccgc gcgttcggct attcctgcga
      241 cgcggacctc ggcgacgcgc gctgcacggt ggatctgacg gaagcagctt tgcacagcag
      301 cggcgtggtg attgcggcaa ccgacgcgcg gcgcttcacg gtgagtggcc tttccgcgtt
      361 cgcctcggga tggttctccg gcggcaagct cctgtggacg gatggcgcca atgcgggacg
      421 cgccatggaa gtaaagcgcc acgcgatttc ggcatcgtcc gtcagcatcg agctgtggca
      481 ggcgatgagc gaaacgctga tggcaggcga tgccttcacg gtgacggcgg gatgcgacaa
      541 gcaattcgcc acctgcaagg caaaattcgt aaaccagatc aatttccgcg gcttccccta
      601 tatgccggga aacgacgtgg tgctgtccta tcccagtcgc ggcgacgcgc tcgatggaaa
      661 aagccgctat ggccattgaa cgtggccgga tcatcgccgc cgcgcggggc tggatcggaa
      721 cgccctatgt gcatcaggcg agtctcaagg ggctcggttg cgattgtctg ggattgctgc
      781 gcggcgtatg gcgcgagctg atgggcgatg aacccgaagc cccgccgccc tactcggcgg
      841 attgggccga agcgcgtggc gacgagacgc tctacgcggc attggcgcgc catttcgcgg
      901 aaatcgatcg catcgatatc gcgccgggcg acatcgcgct tttccgcatg gccgcttccg
      961 gccccgccaa gcattgcggc atcgtcgcgg aactgggcgg catcccgagc ttgatccatg
     1021 cgcgccagaa caagcgcgtg tccgaagaag aattgtcacg tgtcctgcgc cgcaaattgg
     1081 cgttcgcgtt ccgggtctga tccatggcca cgctcattct tgaatcggcc ggccaggcgg
     1141 tgggcgcgct gttcggcggt tccgtcggcg gcatcatcgg ctccgcgctc ggggcgctcg
     1201 ccggcgcgag catcgacaat gcgctttttg ccacacatgg cacggccaat cgcgaaggcg
     1261 cgcggctttc cgatgtgagc atccaggcct cgacggaagg cgccgccatt ccggtcatct
     1321 atgggcgcgt gcgtgtcgcg ggcgagatca tctgggcgac gcggttcaag gagaccgcgg
     1381 tcaccaccag ccagtcggcc ggcgggggca aaggcggcgg tggcggcacc acggtcgttt
     1441 cgacggatta tctctattcc atttcgttcg cggtcggtct ctgcgaaggc attgtgcatc
     1501 agctcgggcg cgtctgggcc gacggcaagc tgttcgatct ttccaaagcg accaccaggt
     1561 tctatccggg gacgcaggat caagggaccg atccgctcat cgaggagatc gaaggagcgg
     1621 gcaatacgcc ggcctatcgc gggctttgcc atattgtgtt cgaagacctg ccattggcgg
     1681 aattcggcaa tcgcatcccg cagcttcagt tcgaattgat ccgctcgctc tcggcggaca
     1741 atcccgattc gctggaaagc cggttgggcg gtgtcgctct cattccgggc gccggcgaat
     1801 tcgtctatgc gaccgacagt gtgaacgccg atgacggcca gggcggcacc gcatcgcaga
     1861 atgtccatgg cgcgagcggt gttgccgaca ttgtcgcctc gctgaacgat ctggaaacgc
     1921 ttgcgcccaa tctgggcgcg gtgtcgctcg tggtcggttg gttcggagac gatctgagag
     1981 ccggtcattg caccgtcaaa ccaggcgtcg aggtcgcgag caaaaccacc tatccccaag
     2041 actggagcgt gaacggcgtt gcgcgcgccg atgcgcatct cgtttcccag gtggaaggcc
     2101 ggccggccta tggcggtacg ccctcggatg cctccgtcgt ccaggcgatt gcggcgttgc
     2161 gcgcgcgcgg ctggcgggtg atgttctgtc cgttcctgtt catggatgtg ccggacggca
     2221 acacgctctc cgatccctac acgggggctg cgggtcagcc ggcctatccc tggcgcgggc
     2281 gcgtcgtttg cgatcccgcg ccgggtgtgg cggggagccc cgacaagacg agtgcggccg
     2341 caacgcaggt aaacgcgttt ttcggcagcg cgagtgcggg cgatttttcc gtcagcggga
     2401 caaaggtgag ttggacgggc ggcaccgatt ggggctggcg ccgcatggtg ctgcattacg
     2461 cgcatctttg cgcggctgcg ggcggcgccg atgccttcct gatcggatcg gaattcacag
     2521 cgctcaatca tgtgcgaagt tcctccacgg cctaccccgc ggttgcggcg ttgaagacgc
     2581 tcgcggcgga tgtgcgcggc atcctcggcg aggccacgag aatcggttat gccgcggatt
     2641 ggtccgaata caacaatcac cagaccggcg atgcacccgg cgcggtgttg tttcatctcg
     2701 acgcgctatg ggccgatgcc aatatcgatt ttgtcggcat cgacaattac atgaaactgt
     2761 ccgattggcg cgacggcacg gcgcacgccg attacgatgc cgtcaatggc cccaccgaca
     2821 ttcacgatcc cgcatacctc gcggcgaata tcgcgggcgg ggaggatttc gactggtatt
     2881 acgcgagcga tgccgaccgc ctcagtcaga cgcgcacgtc catcacggac ggcgtggccg
     2941 gcaaaccctg ggtgtttcgc gccaaggatc ttgtgggctg gtggtcgaat gcacattacg
     3001 accgcgcgaa cggaacggaa gcaggctcgc ccaccgcctg ggtcccgcaa ggaaagccaa
     3061 tctggttcac cgagatcgga tgccccgcca tcgataaggg cgcgaaccag cccaacgtct
     3121 tcttcgatcc gaagtcgagc gaaagtgcgg ttccctattt ttccaacgcc gggcgcgacg
     3181 atctgatcca gcgccgtttc ctggaagcgc atctgaaata ttggagcgac acgggcaaca
     3241 atcccgtctc cggcgtttac acggccagca tggtggatat cgcgaacatc tatgcctgga
     3301 cctgggatgc gcggccgttt ccgttctttc ccgcgcgcgg cgatgtgtgg ggcgataccg
     3361 cgaattaccg tctcggtcat tggctgaatg ggcgtttggg ttcggtgctg ctggccgatc
     3421 tcgtcgccga catctgccgc cacaccgggt tcgccgattt cgataccgcc gatctttccg
     3481 gtctggtgac cggctatctc atcggcgaaa cgatgagccc gcgcgaccaa atcgaaccgc
     3541 tcggattggc gtttcatttc gacgcggtgg agagcgaggg aatcatacgc ttcctcaccc
     3601 gtggccagat accggtttgc tccctgaccg aagacgatct ggcgctgccc gaggaccagg
     3661 cctcgctcgg cgcgaatttc gtgcgcgcgc aggatgccga cctaccgaat gtgtcgcgca
     3721 tctcctacat cgatgccggc gccgattacc gcgaaagcat ggccgaagct catcgcctga
     3781 ccggcggctc caatcgcgtg gcgcaatcgg cattgccgct ggtgctcgac cagggcgagg
     3841 ccatcgggat tggcgagcgg ctgctgcagg acgcatgggt cgcccgcgag accgcgaatt
     3901 ttgcgctgcc gcctgcgcgc atcgcactcg atcctgccga tgaagtgacg ctggccgtcg
     3961 gtggccgcac acggcgattg cgcgtgaccg aaatcggcga tgcgggccct cgcgccatcg
     4021 ctgcggtgat gactgatccc tcgatctacg aaacgctgaa cggtcccgat cgcggcgtcg
     4081 gagctttgac aggcgtaaat gtggcgggcc gcgcgattct cgcctttctc gatctgccgc
     4141 ttttgaccgg caccgaactg gcctatgcgc cgcatgtggc ggcctatgca acgccctggc
     4201 ccggcagcgt gctggtctat cgcagcacca gcgatgccaa ttatgcgctc gacacgcagc
     4261 ttgcgacggc cgcaatcatg ggcgagctgc gcttcgattt ctatgccggt gccaccggcc
     4321 ggtgggacaa gggcaaccag ctctggctga cgctttatgg cggcacgctg tcttgcgtgc
     4381 cggacatcga tgtgttcgcc ggcaccaata cgctcgccat cgaaaatggc gatggcgaat
     4441 gggagatcgt gcagttccgc gacgcggagc tggtcggttc cggccaatgg aagctcacga
     4501 cattgctgcg cggacaggcg ggcaccgaat ccgccatgcg caatccggtc ggggccggcg
     4561 cgcgcatcgt cgtgctggac cgcgacctgc cgcagctcca cctcagtttg gattcccgcg
     4621 cattgccgtt tttctatcgc tggggaccca atggaaaggc gatttccgat gccacttatc
     4681 agacggctca gcggcaattc gttgggattg gcctgcgtcc cttgagtccg gttcagatac
     4741 gggcgcgttg gccgacggtt gcgggcgaca tcgtcatttc ctggaaacgc cgcacgcgca
     4801 tcggcggcga ttcctgggaa caattggacg tgccgctcgg cgaagattcc gaaaattacg
     4861 aagtcgatat tttcaacggc gcgacggtcg tgcgcacgct gcaagcctcc atgccacagg
     4921 cgacctacac gtttgcccag cagacgagtg atttcggcgc ccagcaatgg tcggtcaccg
     4981 tcgcgatcta ccaaatctcg gccgtgttcg gccgcggcgc gcaacgcaaa gcgacgctgt
     5041 tttactgaaa gcacatcatg tccgactcca ccaatctcca gctcccctat ctggatgggg
     5101 cgcaggccca gaaacacgtc accgtcaatg aaagtttacg caaactcgac gcgctggtgt
     5161 tgctcgccat cgaggacaag gatctgacca cgcctccggg ctcgccctcc gatggtgcgc
     5221 gctacattcc gaaagcaacg gcggccggcg cctggtcggg caaggaaaac gcggtcgcgc
     5281 attatgtcga tggcgcgtgg gaattttatc cgccgcgcga aggcttcatc gcctatctgc
     5341 gcgatgagga tcaattgtat ctctacaatg gcagcgcgtg gatcgctctt tcgtcgtcct
     5401 tcgcgacgct ctccgtgagc ggcgccgttc gtgtcggcag cttcatggtt tccgagctcg
     5461 gcagcgcaag tcccgccagc cagatcgtct atgtcagcaa tgaaacaggc ggtgctgtca
     5521 tcgcattctc cgacggcacc aattggcggc gcgtgacgga tcgcgccgtc gtgagctgag
     5581 gattagcgct tcttacggag cttccgcatg gcccataatg tcaaatattc caccgcactg
     5641 aagaattccc ggctcgatca gataacgtcg aaaatcggcg cgtccggcct cttgcgcatc
     5701 tatagcggga cacagccggc caatcccgac attgcggtga catcgcaggt caagctgttg
     5761 gaattcaccg gcgacgcgac cgcctttgcc ggtgcggcgt caggcggggt tctaaccgcc
     5821 aacgcgattg ccgacgccac tgcgctcgcg tccgggacgg cgtcctggtt ccggctttgc
     5881 aaagacgatg ggacaccggc cgttgacggc acggtgggca tctcgggcac cgattgcatc
     5941 atcgacaata tttcgatcac ggccggccaa tccatgccgg tcatctcgct caccatcact
     6001 tcggcaaact gagcagaaag acaaaccatg accacttcgg acgttccggt tcccggcggt
     6061 acgcaacgcg ttccgaccta cgccgtcagc gaagactcca agaccaagga ccttgagcgt
     6121 gttgcgctca atgaatccga tggatcggat cttgcgctcg gcgcgaagct gggcgcgttg
     6181 gcggaaactg cgccggcgac ggataccggg tcgtccggac tgaacgggcg tttgcagcgc
     6241 atcgcgcagc ggctcagtac gctcatcgcc aatcttgcct tcgtattcgg tgccggtacg
     6301 gcgtcggccg cgcagcgtgt gacgctcgcc tccgacgatc ccgcggtcgc tacgcttggg
     6361 tccacatccg gcgcgaaagt gatcgccgat gcgaacggca ctatccagca atatttgcgc
     6421 ggactggtgg cgcaatggat ctccggaacg ctgacgctgg cctcgcgcgc ggcgagcgct
     6481 tcggtggcgc tgacacgcac ggccgatacc aatgcctatg ccgcgaacga tgtcattgga
     6541 gccgcgacgg gatcgacggc tgccatccag ttttcgaaca tgggaccgag cggcgcggtt
     6601 gtgcagatcg tttcgatcga gcttgagatc gactcgactg gggtcatcag cggggaaact
     6661 tcctatcgtg gatatctcta taacgtcacg cccccgtccg cgctcgcaga caacgcggct
     6721 tttgatcttc cgtcgggaga tcgcgcctca tatctgggtt ttctcgactt tggaacgccc
     6781 gtcgatctgg gaagcactct ttatgtgcgt gccacgccgg acaattgctt cgtcaagctc
     6841 tccggcaccg atatcttcat gtatctcgtt acgaacggcc catacacgcc gaccagcgcg
     6901 cgtgtctaca agcccaagct gtttgcggtg gcgtaatgac cgccgattat ttttcgtctg
     6961 atggcgccac ctatcccaat tacgacaaca tcgggccatg cgcattctat gacgccgctg
     7021 acaataagac ctgggattgc cgcgaagcgt ggaacgggtc gcatcgtcaa atcacggtca
     7081 ccgtcttcga tcatggcgcc gggaacatca ccgattatac cgtttccacg aacaccctga
     7141 ccaatgacga ccatggcgag ggcgccttgt gcatggatca tgaggggtat gtgcatctgt
     7201 attggggcgc gcatctaagc cctctgcatc atgccgtaac caccaatccg cgcgatccaa
     7261 cggcatggac aattcgtccc aacatcacga cgacacatcc gggtgcgggt ggggtttctt
     7321 acccccatcc ctgccaggtc ggcagcgtgc aatatctctt attgcgtgac acactcagcg
     7381 gtacccacaa accatgttgc ctgttctaca cgacctcatt gtcgggcggc gttgagacgt
     7441 ggtcttcacg gatcgatatc gcagactacg gtctgggatc ggacggcctc gggacgtggt
     7501 tctattccag cctcatgtat gtggc
//
LOCUS       DASYEG010000126        15134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1345454, whole genome shotgun sequence.
ACCESSION   DASYEG010000126 DASYEG010000000
VERSION     DASYEG010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 15134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15134
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345454"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..1777
                     /locus_tag="VGM72_05300"
     CDS             308..1777
                     /locus_tag="VGM72_05300"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY4264712.1"
                     /translation="MSTQNRKENVTRRGLLKAGGAAVLAAAPLRAKAAAAPPGKLITT
                     LSIYMAEAANKPLPPAVVEKAKQMILDTLAAQISGSQLPPGKMALGFARAYQGDKVAT
                     IPASNMLCGPIEAALVNGMLAHSDETDDTHPPSQSHPGCSLVPSALAAGERFGASGER
                     FIRAVALGYDVGPRVTMTLGKLQYMVATHRSTHAISGTFGSAASAGCMAGLSAQQMRW
                     LLSYTAQSASGLASWQRDTQHIEKSFDFGGMPARNGITAALLVQAGGTALDDIFSGAD
                     NFLEAFKPMNDPNMLIEALGERYEIMRTDVKKWTVGAPIQAPLDALDNLIKKNKFDAA
                     QVQKVVVHVATDEAAIVDNREIPDISLQHLMAVMLVDKTVSFKTAHDEARMKDPAVLK
                     ERAKVQLVRDDGLEKLMPKRVGVVDVTLNDGKMLSERVETVRGTAGNPMTREEMIAKA
                     RDLIVPVLGADKFQKLSDKIFALEGVKNVTELRPLLQTG"
     gene            1821..3215
                     /locus_tag="VGM72_05305"
     CDS             1821..3215
                     /locus_tag="VGM72_05305"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEY4264713.1"
                     /translation="MNKQTTPSKQTSAHAAAKAELEAVLTANPAIQFVDAAIADICGA
                     LRGKRIAVADASKLFESGMQIPLSLHLMDVRGEMMNPSGRGYSDGDPDGTAWPIPGTV
                     TEVWGADPPRAQMLMGLRDNAGAALSYDPRAILERVLARFGELHLTPVAAHELEFYLI
                     DRLRDEKGGPLPPVSPRSGKREDTPSVYGIDDLDRYRGFLTALAEAALLQRVPMSALS
                     KEYSPGQFEANLRHQKNALAASDHAVFLRQIVRAAALSQNVQASFMAKPYPAGLGSGQ
                     HVHLSLVDAAGRNIFDDGTAEGSELLRFAAGGLAALMPESMAFFAPNLNAYRRFQPDM
                     FAPVNRRWGVNNRSAGLRVPTGPSEARRIEHRCAGADANPYLVMAAILAGVHHGIVNR
                     IDPGPPAIGNVSREPDLALPFSLGNALKRLETASVLPSYFGAQAISLYRETKSAELAR
                     FRKIVTAEEYAWYL"
     gene            3313..3720
                     /locus_tag="VGM72_05310"
     CDS             3313..3720
                     /locus_tag="VGM72_05310"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HEY4264714.1"
                     /translation="MRRLAAIAMTWLCLGSIPVSAHHSLAAYTLSNYKTVEGTVKSFE
                     WSNPHAKLSLIAPDANGRTVNWNFEGGSIGRLTTGGFRKGSIAPGDKIVVAYNPRRDN
                     AIGGFFIAVTTANGTTYTTDRYKQLNTPERGLP"
     gene            3908..4927
                     /locus_tag="VGM72_05315"
     CDS             3908..4927
                     /locus_tag="VGM72_05315"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY4264715.1"
                     /translation="MKKLLLALLLLPMSQASAWAQNIPGGDAAAGKALWANDLRCKDC
                     HGGVAEGAFGPDLAGRGLSADQVKQAVRKPWGIMPRFIDEQVSDQELTNLAAYFASMP
                     KVAAPGPWKTEVPANAPAGQRVMITIGCGQCHGDVLIGPRDDMGAVDADINWIKDLVY
                     NHTTAIRTHAQRLGTGNGPVRIHMGNFNPDRVTEAELAQVLAWIKSDVGFRAAMRGRL
                     TKGEAGEKGVTYKLNVQNLGLSGKGLAAEDVSVRLVIPSGTQVVNATGAGYKGVHMDG
                     KQNAQVAEWSWPKAAPQDKQNYTITLSKAGTKADNLRGLIHWEKPMIKPGPTGDEIAI
                     PGAPL"
     gene            5248..6144
                     /locus_tag="VGM72_05320"
     CDS             5248..6144
                     /locus_tag="VGM72_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264716.1"
                     /translation="MRKTTVRLMGALAAASLLISASDGADMSIPPDFSGIWARTTFGW
                     ELPDSGPGPLKNLQRRANGTSDAQMLVGDYMSPILKPGAAKIVKRYGEMSKAGQAFPD
                     PSNQCVSMGTPYILRINEMQMVQQPDRVLILYMQDHQIRTVRLNDSHPQKVTPTWHGD
                     SIGHYEGGTLVVDTVGIKVGPVSMVDQYGTPHSAALHVVERYRLVNESAAKASSAKNE
                     HEYAKADGPAADGDFVDYTYKGEGIQVEFTVEDPQMFTTPWKGAVTYLRAANDWEERV
                     CAENMFEYYDRAALPIADKPDF"
     gene            6157..7032
                     /locus_tag="VGM72_05325"
     CDS             6157..7032
                     /locus_tag="VGM72_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264717.1"
                     /translation="MKRASRIAAKFTRRICGSPGVALLAAVGLLPVAFARGADAIPDF
                     SPNADTGWQLADDEFIAPPMGPGPVASDPAHPYISFYKFPRNPNPTFRVADLDNPILQ
                     PWTREALRKVNERSLSGRYVAIPKERCWPVGVPAFDLLPATPVYFLQQPRTVTMIWTQ
                     DHQVRHVYLNAAHSAKPRPSWFGESVGHYEGDTLVVDTIGLSVKTFVDNYQTPHTGAL
                     HVVERYRMIDGGKTLEVNIHVEDPGAFTTPWNAIQRYRRTQTGPMPETVCAENNDDHF
                     DQDIGPMPVAQRPDF"
     gene            7087..8028
                     /locus_tag="VGM72_05330"
     CDS             7087..8028
                     /locus_tag="VGM72_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4264718.1"
                     /translation="MRSTGRAWGFAVLAVGAALLLNGNFDAGPAFAADAKQEQLSESY
                     RGSKRNDEAYQKIAPFKLFDNLYYVGPGFVSAWLIPTSAGIIMIDTAQEPYVDHVIDS
                     IKTSGFDIKDIKYIVLSHGHLDHFGGAKGIQELSGARVAALDEDWKMMEQFATRPGKN
                     GARPDEAPKRDMVLKEGDTLTLGDETLKFYKLPGHTPGSLAAEFTVYDNGAPHKAFLL
                     GGPGPRNGVTGGQQFVDSMNRVAQIPGIEVAVNVHSWLTSYPYPKGGILERAIALKAR
                     KPGEPNVFVDPESWKQWVTMAQEGAAKYLEAEKRKAA"
     gene            complement(8067..9587)
                     /locus_tag="VGM72_05335"
     CDS             complement(8067..9587)
                     /locus_tag="VGM72_05335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY4264719.1"
                     /translation="MGLSFPRKIQTLFRALAIKIIIVAIIFLFVPIIFYRLFQIVDAQ
                     QSALLQRTVEEKGSLIASVLEPRLAGFETESSDRLQMALDQLLSNGTNIKVLVRPQTG
                     AASNGFLYVASSPQTSADYLEQERDELIRLGIFNRLAPSCDGRSSPTMRFTNPAGHPE
                     ILTSVSAIHVGNNCWVVITSQSAAAILNTSIAQPVWRTPTIHIAIFVYLLSAVIVAWL
                     FIDIWRNIDRFRAAARRIRVHGAGKVTFRQMNTIPELTGVADDFDSLVAALKQSKDFI
                     IQAAEENAHALKAPLAVISQAIEPLKRAVPLSNLQAQRSLDLIERSTARLDLLVSAVR
                     DLEQAAADAIYSNSRPINLSAYLTQLIAAYQTTLTAEGKLLRSEIEPDVHAYATEEAM
                     ESIIENLLENAASFTAPGGTIKVTLRASDSQAHLTIADEGPGVPHDNLPLIFERYFSA
                     RSQVRDMPNVPAQSDNHYGLGLWIVRRNVEGLGGRIAAANRDGGGFAVTASFRAAA"
     gene            complement(9577..10335)
                     /locus_tag="VGM72_05340"
     CDS             complement(9577..10335)
                     /locus_tag="VGM72_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4264720.1"
                     /translation="MNLHYRDNVNERIMIVDDDGMFRESLAQNLSDVGFTIQCFADGP
                     AALRHLTDAGPPDMILLDWKMPGMTGIEVLRELRDRNFETPVVFLTVLSDQIYEEAGL
                     LGGAVDFIEKSRGFAILSRRIELILKGSKSNDALKPAQAQPDIATHGHVRLHRDVNRA
                     FWKDVQVDLTLTEFQMVDYLVTRTGRDVRYRELYDIVHGEGFAAGQGEIGFRANVRAF
                     IKRIRQKFRDVDSDFIQIENYPGFGYRWSDGDGA"
     gene            complement(10316..10885)
                     /locus_tag="VGM72_05345"
     CDS             complement(10316..10885)
                     /locus_tag="VGM72_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264721.1"
                     /translation="MGPRRSIPAVFRTALILFASLAIANCAAPMRLQPEPRPAKITPP
                     PAAQEIPLQAPIVDVPVANQPEAPQVASNMPRPRPSARRPGAPASSSASVSEQEAVLP
                     SELVGYDFSAVLKLLKKPDMIQNSALSIVWTYSQSDCMLQLYFYPDIQTRIFRLLKYD
                     LKDGTGEKPNDSSACMRHMMVRNDEPALP"
     gene            complement(11053..11262)
                     /locus_tag="VGM72_05350"
     CDS             complement(11053..11262)
                     /locus_tag="VGM72_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264722.1"
                     /translation="MPAMNSITDRIQSISARVERYRHLAEALYDRRIAAEVSRVADEL
                     EADLRRLERWQHFSIAAPAPTALAS"
     gene            11552..12283
                     /locus_tag="VGM72_05355"
     CDS             11552..12283
                     /locus_tag="VGM72_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264723.1"
                     /translation="MTMRKTMVLGAAALMAVTSIAPLSAQPRFGNPRTGGAYRDGYEE
                     GRRDGFRDGYRAGYDDGRANRGFDERYGPPPPPPRIANDERDQRWRQRYARTYTYNDD
                     SFYQECRQTVDPAGVIAGALIGGLLGNAVGRGGGRGGATVAGVVVGGALGATLTRNLD
                     CEDRSYAYKTYYDGFNAGRANAVYNWRNPKNGHYGEFRVRDYYNDPDGFRCANYSQQI
                     FVNGRPQEATGRACQQPDGTWTIVS"
     gene            complement(12305..13201)
                     /locus_tag="VGM72_05360"
     CDS             complement(12305..13201)
                     /locus_tag="VGM72_05360"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY4264724.1"
                     /translation="MTVERVIEPRRKDLGGFEVARVLPHAQRRMVGPFVFFDHMGPAD
                     FPAGIPRSVDVRPHPHIGLSTVTYLFDGEIMHRDSLGSQQAIVPGEVNWMTAGRGITH
                     SERFEKARAQGDRLHGIQAWVALPVEDEEADPGFAHHGPSDLPTYQENGLWARLVAGE
                     AFGAKANVKTHSPLFYVHWRLDAGAKAQLPAEYSERAAYIVSGAVEVEGRFYEAGHML
                     VFSAGDPVTVTARQAATIMLLGGEPVGERFVEWNFVSSSKARIDQAKQDWRAGRMKLP
                     DADDKEFIPLPDDPPPPANPMS"
     gene            13347..13814
                     /locus_tag="VGM72_05365"
     CDS             13347..13814
                     /locus_tag="VGM72_05365"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4264725.1"
                     /translation="MERLIDNLIGRYEMGALSRRDLVGALAVLGATGTGAWAAPAGFQ
                     SSTLNHVSITVSDLQRSVEFYQRVFGLALQSENKAAELVQLKLGASHLSIRRADGPKG
                     VDHFAIGLDRFNKDSVIADLKARDATPREDPGAGLHVVDPDGLNVQLIANTAG"
     gene            complement(13833..14735)
                     /locus_tag="VGM72_05370"
     CDS             complement(13833..14735)
                     /locus_tag="VGM72_05370"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEY4264726.1"
                     /translation="MSTPMWPELAFEDWKDTCATLHMWTQIAGKIRLACAAPMNHWWQ
                     VPLYLTSRGLTTSPMPYGARTFEIAFDFCAHRLVITTSDARTESFALQPMSVAAFYRE
                     IMGRLSALGIAVKIWTMPVEIADPIPFEMDSVHASYDGEMAQRFWRILVLADRILTEF
                     RGRFLGKASPVHFFWGSFDLAVTRFSGRTAPPHPGAPNIATWVAQEAYSHEVSSCGFW
                     PGNGGFGKAAFYAYAYPAAPGFSESAGLPVGAYFDKNFGEFILPYDAARQMPDPRGAI
                     LNFLQTTYEAAANTAHWDRKGLER"
     gene            complement(14732..>15134)
                     /locus_tag="VGM72_05375"
     CDS             complement(14732..>15134)
                     /locus_tag="VGM72_05375"
                     /inference="COORDINATES: protein motif:HMM:NF044283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264727.1"
                     /translation="SFHGVSIKQRYPGHAVQAGTIAAQCGGSAYASKYIVVTDEDVDV
                     TNLEHLLWAMVMRTDPVDSIQFLKGTWDSPADAALPPDRRARGDMTHSVAIINACKPY
                     HWRDQFPPSNTPSPETMRKAREKFGWLLEDK"
BASE COUNT         3052 a         4501 c         4807 g         2774 t
ORIGIN      
        1 tctgtggctg ccgccgcagg ttttatgccc gaagagcgct cagcgtaata atccgaggaa
       61 taatacgcat aactcgccca ggccgcgaca atggccaccg tcagaagaaa ctgccacgaa
      121 cgacccatga ccggctccac atgaccctcg atacggccct tttcccgtgc gagagggttg
      181 ggggcgttta tcggaccgcg agggtcttgc ttgcaagagc aaattggctt gagcgaaggc
      241 cacgggccat gtgcaagtcg ggcccgaatg gtacaaacct gtaacaaatc ggaccggggg
      301 agtgaacatg agcacgcaga atcgcaaaga aaacgtcacg cggcgtggat tgctgaaggc
      361 cggaggcgct gccgtgctcg cagcggcgcc gttacgggcg aaggccgccg ccgctccgcc
      421 gggcaagctg ataacaacgc tcagcatcta catggcggag gccgcgaaca agccattgcc
      481 gcccgccgtc gtcgagaaag ccaagcagat gatcctcgac acgctggccg cgcagatttc
      541 gggctcgcaa ttgccgccgg ggaaaatggc gctcggtttt gcgcgcgcct accagggcga
      601 caaggtggcg accatccccg cctccaacat gttgtgcggc ccgatcgaag ccgcgctcgt
      661 caacggcatg ctcgcccatt cggacgagac cgacgacacc catccgccgt cgcaatctca
      721 tcccggttgt tccctggtgc cctcggcatt ggcggcggga gagcgctttg gcgccagcgg
      781 ggagcgtttc atccgcgccg tggcgctcgg ctacgatgtc ggcccgcgcg tgaccatgac
      841 gctcggcaag ctgcaataca tggtggcgac gcacaggagc acgcacgcga tctccggcac
      901 gttcggttcg gcggcgtcgg cgggttgcat ggccggattg agcgcgcagc agatgcgctg
      961 gctgttgtcc tacacggccc aatcggcatc gggtctggca tcctggcagc gcgatacgca
     1021 gcacatcgag aagagcttcg atttcggcgg catgccggcc aggaacggca tcaccgcggc
     1081 gctgctggtg caggcgggcg gcaccgcgct cgacgatatt ttctcgggcg cggacaattt
     1141 cctcgaggcg ttcaagccga tgaacgaccc caacatgctg atcgaggcgc tcggcgagcg
     1201 ttacgagatt atgcgcacgg atgtgaagaa atggacggtc ggcgccccga tccaggcgcc
     1261 gctcgacgcg ctcgacaatc tgatcaagaa gaacaaattc gatgccgccc aggtgcagaa
     1321 ggtcgtcgtc catgtcgcca ccgacgaagc cgccatcgtg gacaatcgcg agattccgga
     1381 catctcgctg cagcatctga tggcggtgat gctggtcgat aagacggtgt cgttcaagac
     1441 cgcccatgac gaagcccgca tgaaggaccc ggcggtgctg aaggaacgcg ccaaggtgca
     1501 actcgtgcgc gacgacgggc tggagaaact gatgcccaag cgcgtcggcg tggtggatgt
     1561 gacgttgaac gacggcaaga tgctgagcga gcgcgtcgaa accgtgcgcg gtacggcggg
     1621 caacccgatg acacgcgagg agatgatcgc caaggcgcgg gatctgatcg tgccggtcct
     1681 gggcgcggac aaattccaga agctcagcga caagattttc gcgctggagg gcgtgaagaa
     1741 cgtgaccgag ctgcggccgt tgctgcagac gggttgattc ttaggacgtc tttcgcgcgt
     1801 caacgcgtta tcatggtatc atgaacaagc agaccacccc atccaagcaa acgagcgcgc
     1861 atgcggcggc caaggccgag ttggaggcgg tgctcaccgc caatccggcc atccaattcg
     1921 tcgatgcggc catcgcggat atttgcggcg cgctcagggg caagcgcatc gcggtcgccg
     1981 acgcgtccaa actgttcgaa tccggcatgc agattccgtt gtcgctgcat ctgatggatg
     2041 tgcgtggcga aatgatgaat ccgagcgggc ggggctatag cgacggcgat ccggacggca
     2101 cggcatggcc cattcccggc accgtgacgg aggtgtgggg ggcggatccg ccgcgcgcgc
     2161 agatgctgat gggcttgcgc gacaatgccg gcgcggccct ctcctacgat ccgcgtgcga
     2221 tcctggaacg cgtgctggcg cgttttggcg aattgcatct gacgccggtc gccgcccatg
     2281 agctggaatt ttacctgatc gatcggttgc gcgacgaaaa aggcggaccc ctgccgccgg
     2341 tctctccgcg ctcgggcaaa cgtgaagaca caccatccgt atacggaatt gatgacctcg
     2401 accgttatcg gggattcctg accgcgctcg ccgaggccgc cctgctgcag cgcgtgccga
     2461 tgagtgcgct cagcaaggaa tattcgcccg gacagttcga agcgaatttg cgccatcaga
     2521 agaacgcgct cgccgcatcc gatcacgcgg tcttcctgcg ccagatcgtc agggctgccg
     2581 cgctgagcca gaacgtccag gcgagcttca tggccaagcc ctatcccgcc ggcctgggat
     2641 cgggccagca cgtgcatctc agtctcgtcg atgcggcggg gcgcaatatt ttcgatgacg
     2701 gcacggcaga aggcagcgag ctgctgcgtt tcgcggcggg aggcttggcg gcgctgatgc
     2761 ccgaaagcat ggcattcttt gcacccaatc tgaacgcata ccggcgtttc cagccggaca
     2821 tgttcgcgcc cgtcaaccgg cgctggggcg tcaacaatcg ttccgcgggt ttacgggtgc
     2881 ccacggggcc gagcgaggcg cgccgcatcg agcaccgatg tgcgggggcc gacgccaatc
     2941 cctatctggt gatggccgcg atccttgccg gtgtgcacca cgggatcgtc aaccggatcg
     3001 atcccggacc gcccgcgata ggcaatgttt cgcgtgagcc cgatctggcg ttgcccttca
     3061 gtctgggcaa cgcgctgaag cggctggaga ccgcgagcgt gctgccgtcc tatttcggcg
     3121 cgcaggcgat ttcgctttat cgcgaaacca aatcggccga gcttgcgcgc ttccgcaaga
     3181 tcgtcaccgc cgaagaatat gcgtggtacc tgtagttttc cgtatcgcgg gaatttggct
     3241 aggataaggg acgcgagcgc aggcaaacat gcgccgcgca taagccgaag aaaattcgcg
     3301 tgggggacat tcatgagaag gctcgccgcc atcgccatga cgtggctttg cctgggttcg
     3361 attccggttt cggcccatca ttcgctcgcc gcctatacac tctcgaacta caagacggtc
     3421 gagggcacgg tgaagagttt cgaatggagc aacccgcatg cgaagctgag tctgattgcg
     3481 cccgatgcga acggtcgcac cgtgaattgg aatttcgagg gcggcagcat cggccggctt
     3541 accaccggcg gtttccggaa gggctccatc gcgccgggcg acaagatcgt cgttgcctat
     3601 aatccacggc gcgacaacgc gatcggcgga ttctttatag ccgtcaccac agccaacggc
     3661 accacctaca ccacggaccg ctacaagcag ctcaatacgc cggagcgcgg tttgccctaa
     3721 gtccaaggac cgccgcaaca aacgcgatct tcgcgagaag gggcgcgctg ttcgcggtta
     3781 tcgtacgcga gcacttttgg cgtgtgtgag cccgtttggc gcgttgcaga aactcggctt
     3841 ggcggtgtaa actaatcgtg aagagccctc ccgccacgtg gagggctgac cgggagggca
     3901 tcgtccaatg aaaaaacttt tgcttgcgct tcttctgctg cccatgagcc aggcgtccgc
     3961 ctgggcgcaa aacattcccg gtggcgatgc cgccgcgggc aaggcgttgt gggccaacga
     4021 tctgcgctgc aaggattgcc atggcggcgt ggctgaaggc gccttcgggc ccgatctcgc
     4081 cggccgcggc ctgagtgcgg atcaggtcaa gcaggctgtc cgcaagccct ggggcatcat
     4141 gccgagattc atcgacgaac aggtcagcga tcaggagctc accaatctcg ccgcctattt
     4201 cgccagtatg ccgaaggtag cggcccccgg tccgtggaag acggaggttc ccgccaacgc
     4261 gccagccggt caacgggtga tgatcaccat cggttgcggc caatgccatg gcgacgtgct
     4321 gatcggaccg cgcgacgaca tgggcgcggt cgatgccgac atcaactgga tcaaggatct
     4381 ggtctataac cacaccaccg ccatccgcac gcacgcacaa cgcctgggga ccggcaatgg
     4441 cccggtacgc attcacatgg gcaattttaa tccggatcgc gtgaccgaag ccgagttggc
     4501 gcaggtgctt gcctggatca agagcgatgt gggtttccgc gcggcgatgc gcggccgtct
     4561 taccaagggc gaagccggcg agaagggcgt cacctataaa ttgaatgtcc agaaccttgg
     4621 tctttccggc aagggactgg ccgccgaaga cgtgtcggtt cgccttgtca tcccgtccgg
     4681 cacgcaagtc gtgaatgcga ccggcgccgg atataagggc gtccatatgg atggcaagca
     4741 gaacgcgcaa gtcgccgaat ggtcctggcc gaaggccgcg ccgcaagaca aacagaatta
     4801 tacgatcacc ttgtcgaagg ccggcaccaa ggcggacaat ctgcgcggtt tgatccattg
     4861 ggaaaaaccg atgatcaagc caggcccgac cggcgatgag atcgccattc caggcgcgcc
     4921 gctttagccg cgtctataag gtatgaatgg gccggctctg cgaacgtggg gccggccgat
     4981 ttttaccgcc ggatctatcc ggcgtttacg gtgaagtgac gctgcgagtc cgggtcttat
     5041 aggcttgtcc ggggccccgg cgatcagaca atgatctccc tggttaagac ttggagccat
     5101 ttcagaaaac tgggggccca cccagtccta cccccatctt ggcaatggcg tttgcccctg
     5161 agacggattc ttctccggtg agagtgcggc tcgtaatata gtgtggtccc ggcggccgga
     5221 cggagggatt ttcgggtgcg gtttgttgtg cggaaaacga cggtgcggct gatgggggct
     5281 ctcgctgccg cgtccttgct gatctcggcg agtgacggcg ccgatatgag cattccaccg
     5341 gatttcagcg gtatctgggc gcgcaccaca ttcggctggg agttgcccga ttcggggccg
     5401 gggccgctga agaatctgca gcgccgcgca aacgggacga gcgatgcgca aatgctggtc
     5461 ggcgattata tgagcccgat cctgaaaccg ggggccgcaa aaatcgtcaa gcggtacggt
     5521 gagatgtcaa aggcgggcca ggcctttccc gatccctcca atcaatgcgt ctccatggga
     5581 acgccctaca tcctcagaat caacgagatg cagatggtgc aacagccgga ccgcgtcttg
     5641 atcctgtata tgcaggacca tcagatccgc acggtcaggc tgaacgattc gcacccgcag
     5701 aaggtgacgc ccacctggca tggcgattcc attggccatt acgagggcgg gacgctggtg
     5761 gtcgatacgg ttggcatcaa ggtcgggccg gtgtcgatgg tggatcagta cggcacgccg
     5821 cacagcgcgg cgctgcacgt ggtggaacgc tatcgccttg tgaatgagtc ggccgcgaaa
     5881 gcctcgagcg ccaagaacga acacgaatat gccaaggccg acggcccggc cgccgatggc
     5941 gactttgtcg attacaccta caagggcgag ggcatccagg tcgagttcac ggtcgaggat
     6001 ccgcaaatgt tcaccacgcc gtggaagggt gcggtcacct atctgcgcgc ggcgaatgat
     6061 tgggaagagc gcgtctgcgc cgagaacatg ttcgaatatt acgaccgcgc ggcgttgccg
     6121 attgcggata agccggattt ctgaaaggaa aaagcgatga agcgcgcatc gcgaattgcg
     6181 gcgaaattca cccggcgcat ctgcggatcg ccgggcgtcg cgctattggc ggctgtggga
     6241 ttgctgcccg tggcgttcgc gcgcggcgcc gatgcgattc cggatttctc ccccaatgcc
     6301 gataccggct ggcaattggc cgatgacgaa ttcatcgcgc cgccgatggg gccagggccc
     6361 gtcgcatccg atccggcgca cccctacatc agcttctaca aattcccgcg caatcccaat
     6421 cccaccttcc gggtagccga tctcgacaat ccgatcctgc agccctggac gcgtgaggcg
     6481 ctgcgcaagg tcaacgagag atcgctgtca ggacgctatg tggccatccc gaaggaaaga
     6541 tgctggcctg tcggcgttcc cgcgttcgat ctcttgcccg cgacaccggt ctatttcctg
     6601 cagcagccca gaaccgtgac gatgatctgg acccaggatc atcaggtgcg tcacgtctat
     6661 ctgaatgcgg cgcattcggc gaaacccagg ccgagctggt tcggcgaatc cgtcggtcat
     6721 tatgagggcg acacgcttgt cgtcgatacg atcgggctgt cggtcaagac gttcgtggac
     6781 aattaccaga ccccgcatac cggtgcgctg cacgtggtcg agcgttatcg catgatcgat
     6841 ggcggcaaga cgctggaggt caatatccat gtcgaagacc cgggggcctt caccacgccg
     6901 tggaacgcga tccagcgcta ccggcgcacg caaaccgggc caatgcccga aacggtgtgc
     6961 gccgagaaca atgacgatca tttcgatcag gacatcggac cgatgccggt cgcgcaaagg
     7021 ccggattttt gaggccggat tttttaggcc ggattttgag gccggatttt gaggagagcg
     7081 ggaaagatga gatcgaccgg acgcgcatgg ggatttgccg tgctcgccgt tggcgcggcg
     7141 ctgctgctga acggaaattt cgatgccggg ccggccttcg cggcggacgc gaaacaggag
     7201 cagctgagcg aatcctatcg cggcagcaag cgcaacgacg aggcgtacca gaaaatcgcg
     7261 ccgttcaaac tgttcgacaa tctctattat gtcggaccgg ggttcgtgtc ggcgtggctg
     7321 atcccgacct cggccggaat catcatgatc gacaccgctc aggagcccta tgtcgatcac
     7381 gtcatcgaca gcatcaagac gtccggcttc gacatcaagg acatcaaata tatcgtgctc
     7441 agccacggtc atctcgatca tttcggcggc gctaagggca tccaggaatt gtccggcgcg
     7501 cgcgtggccg cgctcgacga agattggaag atgatggagc aattcgcgac gcgcccgggc
     7561 aagaacggcg cacggcccga cgaggcgccc aaacgcgaca tggtcctgaa agaaggcgat
     7621 acgctgaccc tcggggacga gacgctgaaa ttctacaagc tgccaggaca cacgccgggt
     7681 tcgttggcgg ccgaattcac cgtctacgac aatggcgcgc cgcataaggc gttcctgctt
     7741 ggcgggccgg gtccgcgcaa tggtgtgacc ggaggacagc aattcgtgga cagcatgaac
     7801 cgcgtggccc agattcccgg catcgaagtc gccgtgaacg tccatagctg gctgacgtcc
     7861 tatccctatc cgaagggcgg catcctggaa cgcgcgatcg cgttgaaggc gcgcaaaccc
     7921 ggcgaaccca atgtcttcgt cgatcccgaa tcctggaagc aatgggtgac gatggcccaa
     7981 gagggtgccg cgaagtatct cgaagcggag aagcgcaagg cggcgtagta ggcgtctcgg
     8041 cgctgcttca accggattcc gcaaaatcag gcggcggcgc ggaagctcgc cgtcaccgca
     8101 aagccgccgc cgtcgcgatt ggccgcggca attcgcccgc ccaggccttc gacattgcgc
     8161 cgcacgatcc acaggccaag cccgtaatga ttgtcgctct gcgccggaac attcggcatg
     8221 tcgcggacct gagaacgcgc ggaaaaataa cgctcgaaga tcaacggcag attgtcgtgg
     8281 ggtacgcccg gaccttcgtc ggcgatggtg agatgggcct gcgaatccga tgcccgcagc
     8341 gtcaccttta tggtgccgcc cggcgcggtg aagcttgccg cgttctcgag cagattttcg
     8401 atgatggatt ccatggcttc ttcggtggcg taggcatgca cgtcgggttc gatttccgaa
     8461 cgcagcagct tgccttcggc ggtgagcgtc gtctgatagg cggcgataag ctgcgtgaga
     8521 taggccgaca ggttgatggg acgcgaattg gaatagatcg cgtcggctgc cgcctgctcc
     8581 agatcgcgca ccgcggaaac cagaaggtcg agccgcgccg tcgaacgctc gatcagatcg
     8641 agactccgct gggcctgcag attgctgagg gggaccgcgc gcttcaaggg ttcgatcgcc
     8701 tgcgaaatca cggcgagggg ggccttgagt gcgtgggcat tttcttcggc ggcctggatg
     8761 atgaaatcct tcgactgttt caatgccgcg accagcgaat cgaaatcgtc cgcaacgccc
     8821 gtcagttccg gaatcgtgtt catctgccgg aacgtcacct tgcccgcgcc gtggacgcgg
     8881 atcctgcggg cagcggcgcg gaagcggtcg atgttgcgcc agatgtcgat gaacagccaa
     8941 gccacgatca ccgcgctcaa aagatagaca aagatcgcga tatggatggt cggcgtgcgc
     9001 cagaccggtt gggcgatgga ggtgttcaga atcgccgccg ccgattgcga ggtaatcacc
     9061 acccagcaat tgttgccgac atggatggcc gacaccgagg tgagaatctc cggatgtccc
     9121 gcagggttgg tgaagcgcat cgtgggactg ctgcgcccgt cgcagctcgg cgcgagacgg
     9181 ttgaagatcc ccaagcggat cagctcgtcg cgctcctgtt cgagatagtc cgccgatgtt
     9241 tgcggcgagg aggccacgta aagaaagccg ttcgatgccg cacctgtttg cgggcggaca
     9301 aggaccttga tgttcgttcc gttcgagaga agctggtcca gcgccatctg cagccggtcg
     9361 gacgattcgg tttcaaaccc ggcaagacgc ggttcgagca ccgaggcgat cagcgagcct
     9421 ttttcctcga ccgtacgctg gagcaaggcg ctttgctgag catcgacgat ctggaacaag
     9481 cggtagaaaa tgatcggcac aaaaaggaat ataatcgcaa cgatgatgat cttgatcgcg
     9541 agcgcgcgaa agagtgtctg aatcttgcgg gggaagctaa gccccatcgc catcgctcca
     9601 ccggtaaccg aaaccggggt aattctcgat ctggatgaaa tcggaatcca catcccggaa
     9661 tttctgccgg atgcgcttga tgaaggcgcg gacattggcg cgaaagccga tttcgccctg
     9721 accggccgca aagccttcgc catgcacgat gtcgtaaagc tcgcgatagc gcacgtcacg
     9781 acccgtgcgc gtgaccaggt aatcgaccat ctggaattcg gtcagcgtga gatcgacctg
     9841 cacgtccttc cagaaggcgc ggttgacgtc gcggtgcagg cgcacatggc cgtgcgtggc
     9901 gatgtctggc tgggcttggg ccggtttgag cgcgtcgttg ctcttcgatc ccttgagaat
     9961 caactcgatc cggcgcgaga gaatcgcaaa gccgcgcgat ttctcgatga aatccacggc
    10021 ccccccgaga aggcccgcct cttcatagat ctgatcgctg aggaccgtca ggaaaaccac
    10081 cggtgtctcg aaattgcgat cgcgcaattc gcgcagcact tcgatacccg tcatgccggg
    10141 catcttccaa tcgagcagga tcatgtccgg cgggccggcg tccgtcaggt ggcgaagcgc
    10201 cgccggaccg tcggcaaagc attgaatggt gaagccgaca tccgacagat tctgtgccag
    10261 ggattcccgg aacatgccat cgtcatcgac gatcatgatg cgttcgttca cattgtcacg
    10321 gtagtgcagg ttcatcgttc ctcaccatca tgtggcgcat gcatgcgctt gaatcattgg
    10381 gtttttcgcc ggtgccgtcc ttgagatcat atttcagcaa gcggaagatc cgagtctgaa
    10441 tgtcgggata gaaataaagc tggagcatgc agtcggattg ggaataggtc cacacgatcg
    10501 aaagcgcgct gttctgaatc atgtccggct tcttcagcag cttcaggaca gcggagaaat
    10561 catagccgac aagctcggat ggaaggacgg cctcctgttc ggaaacggaa gcggacgatg
    10621 acgcgggcgc tccaggtctt ctggccgacg gtcgcggtct tggcatattg ctcgcgacct
    10681 gcggtgcttc gggttgattt gccacaggca cgtccacgat cggggcctgc aaggggattt
    10741 cctgcgcagc cggaggcggg gtgatcttcg ccggacgcgg ttcgggttgg agccgcatgg
    10801 gcgccgcgca attggcgatc gcaagcgagg cgaacaggat cagcgcagta cggaaaactg
    10861 cgggaattga tcgtctcggg cccataatgc gccaccttca caaagcactg cgaaccccga
    10921 gcgctccccc cacctgttcc aataccgcac atatccttaa aaagtcacta ctaacttgca
    10981 tggaagtctt acccatgtcc taacacgcct tccggtccgg aacgcggcat agcacgcgat
    11041 taatgaggaa aatcaggagg caagagccgt gggggcgggt gccgcgatcg agaaatgctg
    11101 ccagcgctcc aaccgccgaa gatcggcctc gagttcatcg gcaacgcgcg aaacttccgc
    11161 agcgatgcgc cggtcgtaca gcgcctccgc caaatgccgg taacgttcca cgcgggcgga
    11221 aatgctctgg atacggtcgg tgatggaatt cattgccggc atggaaattc ctctactcta
    11281 ctctcacaaa cgccgtcatc atgcgggaga aacgcagaca ctacgctcca aaaccgccgc
    11341 atcgcgaagg ggggtgaact ggccgcgagt cgttggaaaa ttcgctcggc ctgtgtttgc
    11401 cccgcatgat gagcgcatga gaaccatagg gaatttccgt atcgattggt gtgatctatt
    11461 gaagacaaag ccggcgcaaa tcatggtctt ttgccgcaaa attgccatca aatggaatga
    11521 aattcatatt ggaatccggg ggcaaaacat catgacgatg cgtaaaacaa tggtgcttgg
    11581 cgcggcggct ctcatggctg tgacttcgat tgcgccgctg tcggcacagc cgcgtttcgg
    11641 caatccacgc accgggggtg cgtatcgcga cggttatgag gaaggccgcc gcgatgggtt
    11701 tcgcgatggc tatcgcgccg gctatgacga tggccgcgcc aacaggggct tcgacgaacg
    11761 ctacgggcct ccgcctcctc cgccgcgcat cgccaatgac gaacgcgatc agcgctggcg
    11821 gcagcgttat gcacgcacgt atacctataa tgacgattca ttttaccagg aatgccggca
    11881 aacggtcgat ccggccgggg tgatcgccgg cgcgctgatc ggaggattgt taggaaacgc
    11941 cgttggacgc ggcggcggac gtggcggcgc cacggttgcg ggcgtggtcg tgggcggtgc
    12001 gctgggcgcg acgctgacgc gcaatctgga ttgcgaagac cgcagctacg cctacaagac
    12061 ctattatgac ggcttcaacg cgggccgggc gaacgcggtc tataattggc gcaatccgaa
    12121 gaacggccat tatggggagt tccgagtccg cgattactac aatgatccgg atggctttcg
    12181 ctgcgccaac tactcgcaac agatcttcgt caatggccgt ccgcaggaag ccacgggccg
    12241 cgcttgtcag cagccggacg ggacgtggac gatcgtgagc tgaagccggt acggcatcgg
    12301 accatcagga catcgggttt gccggcggcg gcggatcgtc aggcagcggg atgaattcct
    12361 tgtcgtccgc gtcgggaagc ttcatccgcc ccgcccgcca gtcctgtttg gcctggtcga
    12421 tgcgcgcctt cgaagacgaa acgaaattcc attcgacgaa gcgctccccc acgggttcgc
    12481 ccccgagcag catgatggtc gcggcctggc gcgccgtgac ggtgacggga tcgccagccg
    12541 agaagaccag catgtggccc gcctcataga aacggccctc gacctcgacc gcgccggaaa
    12601 cgatataggc cgcgcgctcg gaatattcgg cgggcaattg cgcctttgcg cccgcatcaa
    12661 gccgccaatg cacatagaac aaaggcgagt gggttttgac gttcgctttg gcgccgaagg
    12721 cttcacccgc cacgagccgg gcccataggc cgttctcctg ataggtcggc aaatcgcttg
    12781 gcccatgatg cgcgaagccg ggatcggcct cctcgtcttc cacgggcaag gcgacccacg
    12841 cctggatgcc gtgcagccgg tcgccttgcg cgcgcgcctt ctcgaagcgc tcggagtggg
    12901 tgatgccgcg ccccgcggtc atccaattga cctcgccggg aacgatggcc tgctgcgagc
    12961 ccagactgtc gcgatgcatg atctcgccgt cgaacaggta ggtgacggtg gaaagaccga
    13021 tatggggatg ggggcggaca tcgacgctgc gcggaatgcc tgcggggaaa tccgccggac
    13081 ccatatggtc gaagaacacg aaggggccaa ccatgcgccg ctgcgcgtgc ggaagcacgc
    13141 gggcgacctc gaacccgcca aggtccttgc gccgcggctc gatgacacgt tccaccgtca
    13201 tgatgtccgt ctccctttgt gctggcgctg agcttggcgc agacgcaaac ggcattcaat
    13261 gccgggcttt gcgtctttct cgcccctcga aggaagcggt gatagcatcg catcgttcgg
    13321 atgcgcatca atgtttggag gctcggatgg aacggctcat cgacaatttg atcgggcgtt
    13381 acgaaatggg tgccttgtcg cggcgagatt tggttggggc gctggcggtc ttgggcgcaa
    13441 caggcactgg cgcatgggcc gcaccggccg gatttcagag cagcacgctg aatcacgttt
    13501 ccatcacggt cagcgatctt cagcgctcag tggaattcta tcaacgcgtc ttcggtttgg
    13561 cgctgcagag cgagaacaag gcagcagagc tcgtgcagtt gaagctcggc gcgagtcatc
    13621 tttcgatccg gcgtgccgat ggacccaaag gcgtggatca tttcgccatc gggctcgacc
    13681 ggttcaacaa ggattccgtc atcgccgatt tgaaggcccg tgatgcgacc ccgcgcgaag
    13741 accccggcgc cggcctgcac gtggtcgatc ccgatgggct gaacgtgcag ctcatcgcta
    13801 acaccgccgg ataagaagcg tccgggccga gatcagcgct ctaaaccttt ccggtcccaa
    13861 tgtgcggtgt tggctgcggc ctcgtaggtc gtctgcagaa aattcaggat cgcgccgcgc
    13921 gggtcgggca tctgccgcgc ggcatcatag ggcagaatga attcgccgaa atttttgtcg
    13981 aaataggcac ccacaggcaa acccgcgctc tcgctaaatc cgggcgcggc cggataggcg
    14041 taggcataga acgcggcttt gccgaaacca ccattgccgg gccagaagcc gcaactgctc
    14101 acctcatgcg aataggcttc ctgagccacc catgtcgcaa tgttcggcgc gccgggatgc
    14161 ggcggcgccg tgcgtcctga aaatcgcgtc acggcgagat cgaagctgcc ccagaagaaa
    14221 tgcaccgggc tggccttgcc taaaaaccgc ccgcggaatt cggtgagtat ccgatccgcc
    14281 agcaccagga tgcgccagaa tcgctgtgcc atctcgccat cataggacgc gtgcacgctg
    14341 tccatctcga acgggatggg atcggcaatc tccaccggca tggtccagat tttcacagcg
    14401 atgccgagtg cgctgaggcg gcccatgatc tcccgataga aggcggcgac gctcatgggc
    14461 tgaagcgcga aactttccgt cctcgcatcg cttgtcgtga tgacgagccg gtgggcgcag
    14521 aaatcgaacg cgatctcgaa ggtgcgcgcg ccataaggca tgggcgaggt ggtcaggcct
    14581 cggctggtca gatagagcgg cacctgccac caatgattca tcggcgccgc gcaagccaga
    14641 cggattttgc ccgcgatctg cgtccacatg tgaagcgtgg cgcaggtgtc tttccaatcc
    14701 tcgaacgcaa gctcaggcca catgggcgtg ctcatttgtc ctccagaagc cagccgaatt
    14761 tctcgcgcgc cttgcgcatc gtctcgggcg aaggcgtgtt ggagggcggg aattgatcgc
    14821 gccaatgata gggcttgcag gcattgatga tggcaaccga atgcgtcatg tcgcccctgg
    14881 cgcgtctgtc cggcggcaag gccgcatcgg cgggcgaatc ccacgtccct ttcaggaatt
    14941 ggatcgagtc cacgggatcg gtgcgcatca ccatcgccca taacagatgt tccagattgg
    15001 tgacgtcgac atcctcatcc gtcaccacga tatatttcga cgcataggcc gagccgccgc
    15061 attgcgcggc aatcgttccg gcctgcacgg cgtggcccgg ataacgctgt ttgatggaga
    15121 cgccgtggaa cgag
//
LOCUS       DASYEG010000127         5108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3664433, whole genome shotgun sequence.
ACCESSION   DASYEG010000127 DASYEG010000000
VERSION     DASYEG010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3664433"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..777
                     /locus_tag="VGM72_05380"
     CDS             13..777
                     /locus_tag="VGM72_05380"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY4264728.1"
                     /translation="MSKRLVFAGVVAVALAGSDAARAIEINVYSAGAVQEGERALAVS
                     FTKMTGNKVSFTVGTVGQIQEKLKSGAPADVIVVSAPALAQLEKSGDVRASSAVALGR
                     IGIGVGVKDGAAVPDISTPEKFREAMLAAKSVTYMDPAQGASSGIATAKIMKDLGIAE
                     EVAKKTKLTETGYSADRVASGEVEIAIQNVSEIVPVNGVKLAGMLPEPLQVYTAYAAG
                     VATKSVAPKEATDFIRYLARAESAGDWKEAGIDPAH"
     gene            908..1612
                     /locus_tag="VGM72_05385"
     CDS             908..1612
                     /locus_tag="VGM72_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4264729.1"
                     /translation="MATIALVDDDKNILASVTMLLEQEGYHVRSYTDGASALTALTAT
                     PPDLAILDIKMPRMDGMEVLRRLRQAMEIPVIFLTSKDEEIDELMGLNAGADDYIRKP
                     FSQRLLLERVKAVLRRAEGKGSAAQAQPGEDKQIMTRGKMTLDPLRHECTWDGKPVRL
                     TVTEFLILQSLALRPGFVKSRDNLMDAAYDDQVYVDDRTIDSHIKRLRKKFKAVDDNF
                     DSIETLYGVGYRYKEN"
     gene            1698..3323
                     /locus_tag="VGM72_05390"
     CDS             1698..3323
                     /locus_tag="VGM72_05390"
                     /inference="COORDINATES: protein motif:HMM:NF025131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stimulus-sensing domain-containing protein"
                     /protein_id="HEY4264730.1"
                     /translation="MSADLPYRPRRRFSALTRRILFFNVIALLILVAGVLWVQSNRIG
                     LVQERISGIRDQALIVAGALAEYAADPNRRAIQPTQAEPLLRQLISPTHLRARVYSTT
                     GRLQLDTRNLLARNVVTTEELPPPEPSNPIVAFVYRSVAAVRHLYDGLMGVRPLAHLE
                     PYFEAGQNGRVYDEVNRALMSETGSSVRVNEQDKLVISVAVPIRRFATLYGVLMMTTE
                     SGDIDDILKAERTALVEVFAVAFVALLFSAVYLAGFIARPILRLAGAAERVRRGRAGR
                     DAIPTLDGRDDEIGELADSLSAMTRALYERIDAIESFAADVAHELKNPLTSLKSAIEM
                     FERASAGENRDRLVQVIRNDVKRIDRLITDISDASRLDAELSRGLSEPIDLSKLLETI
                     VDIYSGMNVPRGVQIRFAGDAHKPLLVQGLSTRLGQVFRNLIDNSISFSPDGGTVLVQ
                     AQMANGNVRATVEDEGPGIGEDDLERIFGRFYTERPPEHGFGKNSGLGLAIARQIVAS
                     HGGRIWAENKFDSEGKRAGARFIVELPLLRNDT"
     gene            3320..3889
                     /locus_tag="VGM72_05395"
     CDS             3320..3889
                     /locus_tag="VGM72_05395"
                     /inference="COORDINATES: protein motif:HMM:NF019118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264731.1"
                     /translation="MMSPVLVHASCVVLGNAAAPFGAAIEGAVLLMGASGAGKSDVAL
                     RLIAMGATLLADDQTALFAEGGRLFAQGISSLHGQMEVRGVGILKADAITRAPVILAV
                     RLDETSKIPRLPEPLTHKLPQGLEGCEEPPFLVLRPFEASTPAKIAAAGAGLARGAFV
                     AGLVSPSSGRFFNRNAAIFPAPSPTRTKT"
     gene            3886..4296
                     /locus_tag="VGM72_05400"
     CDS             3886..4296
                     /locus_tag="VGM72_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEY4264732.1"
                     /translation="MIGIVIVTHGQLAAELLSAAQHIQGHQSHVKTVCIGPNDDMEER
                     RDEITAAIKAVDLGSGVVLVTDMFGGTPCNLALTHLQKGKVEVLAGANLPSLIKLISV
                     RITLPLEKAVCEAIESGRKYMRAGSAELAGSPGK"
     gene            4293..4577
                     /locus_tag="VGM72_05405"
     CDS             4293..4577
                     /locus_tag="VGM72_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEY4264733.1"
                     /translation="MNTPQTVRTIATIVNKRGLHARASAKFVETAARFQSEVTVTKGD
                     TSVSGRSIMGLMMLGASLGTAIELTAEGPDASSAVGALTSLIAAKFHEDA"
     gene            4633..>5108
                     /locus_tag="VGM72_05410"
     CDS             4633..>5108
                     /locus_tag="VGM72_05410"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEY4264734.1"
                     /translation="MTAAQQLRTRLAGVLCACLISAPAAGLAQVAPHPALWRVARGPA
                     TIYLFGSLHVLPAGYSWSTPEIDAAKAASEMFIFEVPVDEAALKDERQFIVENGLLGR
                     GQSLRGVLSANEFQTYSAVLKRAGLKPQQFERYRPWLAAVVVGLAYLHGGNLDSLRG"
BASE COUNT          952 a         1584 c         1612 g          960 t
ORIGIN      
        1 atgaggaaaa cgatgtccaa aaggcttgtc ttcgccgggg tcgtcgcagt tgcgctggct
       61 ggttcggatg cggcgcgtgc gatcgagatc aatgtctatt ccgcgggcgc ggtgcaggaa
      121 ggcgaacgcg cgctggccgt gagtttcacg aagatgacgg gcaacaaggt gagttttacc
      181 gtcggaaccg tggggcaaat ccaggagaag ctgaaatcgg gcgcgccggc cgacgtcatc
      241 gtggtgtccg ccccggcact ggcacaactg gagaaatccg gcgacgtgcg ggcttcgagt
      301 gccgtggcct tgggccgtat cgggatcggc gtgggcgtca aggacggtgc ggccgtgccc
      361 gacatctcga cgcccgagaa attccgggag gccatgctgg cggcgaaatc cgtgacctat
      421 atggacccgg ctcagggcgc ctcgtccggc attgccacgg ccaagatcat gaaggacctc
      481 ggcatcgccg aagaggtcgc gaagaagacc aaactgaccg agacaggcta ttccgcggac
      541 cgtgtcgcgt ccggcgaggt ggagatcgcg atccagaacg tcagcgagat cgtgccggtc
      601 aacggcgtga aattggcggg catgctgccg gaaccgctgc aggtgtacac ggcctatgcg
      661 gcgggggtgg caaccaagag cgtcgcgccc aaggaagcca ccgatttcat ccgttatctg
      721 gcgcgggccg aatccgcggg cgactggaag gaagccggta tcgatccggc ccattgatcc
      781 ctatttcagc catcttggga cgcgatattc ccttcgtcgt ggagatgacc gacagagccg
      841 gctccgagag gcgctgcgct ttcgccgccc ctgccctata ttacaattgc aacaaaggtg
      901 accttccatg gcgactatcg ccctcgtgga cgacgacaag aacatcctgg cctcggtgac
      961 catgctgctg gaacaggaag gctatcacgt ccgcagctat accgacggag cctcggcctt
     1021 gaccgcgctc acggccaccc ctcccgacct cgccattctg gacatcaaaa tgccgcgtat
     1081 ggacggcatg gaagtgctga ggcgcctgcg tcaggcgatg gagattcctg tgatcttcct
     1141 gacctccaaa gacgaagaga tcgacgaact gatgggcctg aacgcgggcg ccgacgatta
     1201 catccgcaag cccttttccc agcgcctgct gctggagcgc gtcaaggcgg tgctgcgccg
     1261 cgccgaaggc aaaggcagcg ccgcgcaggc acagccgggc gaagacaagc agatcatgac
     1321 gcgcgggaag atgaccctcg atcccctgcg ccatgagtgc acctgggacg gcaagccggt
     1381 gcggctgacg gtgaccgaat tcctgatcct gcagtcgctg gcgctgcgtc ccggtttcgt
     1441 caaaagccgc gacaacctta tggacgccgc ttatgacgat caggtctatg tcgacgatcg
     1501 caccatcgat tcgcacatca agcgtctgcg caagaaattc aaagcggtcg atgacaattt
     1561 cgactccatc gagacgcttt acggggtcgg ctaccgctat aaggaaaact gaggggcggc
     1621 tgtcttgaaa tgaggacatt gctctgaatc taaggccgcg attccagacc gaaacccaac
     1681 gcccatcgcc tcgcccgatg agcgccgatc tgccctatcg cccacgccgg cgattttcgg
     1741 cgctgacgcg ccggatcctg ttcttcaatg tcatcgcgct gcttatcctg gtggccggcg
     1801 tgctctgggt gcaatccaac cgcatcggtc tggtccagga gcgcatctcg ggaatccgcg
     1861 accaggcgct gattgtcgcc ggcgcgctcg ccgagtatgc cgccgatccc aaccgccgcg
     1921 ccattcagcc gacgcaggcc gagcccttgc tgcgccagct catctcaccc acccatttgc
     1981 gcgcgcgcgt ctattcgacg acaggccgtc tgcaactgga cacgcgcaat cttctcgccc
     2041 ggaacgtggt gaccacggaa gaattgccac cccccgaacc ctccaacccg attgtcgctt
     2101 tcgtctatcg ctcggttgcg gccgtcaggc acctttatga tggactgatg ggcgtgcggc
     2161 cgctcgccca tctggaaccc tatttcgagg ccgggcagaa cggccgcgtt tatgacgaag
     2221 tcaaccgcgc cctgatgagc gagaccggca gcagtgtgcg cgtcaatgag caggacaagc
     2281 tggtcatttc ggtcgccgtg ccgatccgcc gcttcgctac tctttacggc gttctcatga
     2341 tgacgaccga atcgggggat atcgacgata tcctgaaggc cgagcgcacc gctttggtgg
     2401 aggtgttcgc ggtggcgttc gtagcgctgc tgttttcggc ggtgtatctc gcgggcttca
     2461 tcgcccggcc gattctgcgg cttgccggtg cggcggagcg cgtccgccgg ggccgcgccg
     2521 gccgcgatgc cattccgacg ctcgacggcc gcgacgacga gatcggcgag ttggcggatt
     2581 ccctctccgc catgacgcgc gctctctatg agcgcatcga tgcgatcgag agcttcgcgg
     2641 ccgatgtcgc ccatgagctg aagaaccctc tgacttcgct gaaaagcgcc atcgagatgt
     2701 tcgaacgcgc cagcgcaggc gaaaaccgcg atcggctggt gcaggtgatc cgcaacgatg
     2761 tgaagcgcat cgaccggctg atcaccgaca tttcagatgc ctcacgcctc gacgcggaat
     2821 tgtcccgtgg actttccgag cccatcgacc tttccaagct gcttgagacg atcgtcgata
     2881 tctatagcgg catgaacgtt ccgcgcggcg tgcaaatccg ctttgccggc gatgctcaca
     2941 aaccgcttct ggtgcaaggg ctaagcaccc gtctcgggca ggtgttccgc aatctgatcg
     3001 acaattcgat ttcgttcagt ccggatggcg gcacggtgct tgtgcaggcg cagatggcca
     3061 acgggaatgt gcgcgccacg gtcgaggacg aaggtccggg tatcggcgag gacgacctcg
     3121 agcgcatctt cgggcgcttt tataccgaac gtccgccgga gcacggcttt ggaaaaaatt
     3181 ccggactcgg cctcgccatc gcgcgacaga tcgtggccag ccatggcgga cgcatttggg
     3241 cggagaacaa attcgacagc gaaggcaaac gcgccggcgc gcgcttcatc gtcgaactgc
     3301 cgctattgcg gaatgacaca tgatgtcccc ggttctggtt catgcgagct gcgtggttct
     3361 gggcaacgcc gcggccccct tcggggctgc gatcgagggc gcggtgctgc tcatgggcgc
     3421 cagcggcgcg ggcaaatcgg acgtggcgct tcggctgatc gcaatgggcg cgacgcttct
     3481 ggcggacgac cagacagcgc tcttcgccga ggggggccgt cttttcgcgc aaggtatttc
     3541 ctccttacac ggacagatgg aggtgcgggg cgtgggcatc ctcaaggcgg acgccattac
     3601 gcgcgcgccc gtgattctgg ccgtgcggct ggacgaaacc tcaaagatac cgaggctgcc
     3661 tgaaccgctc acccataagc ttccccaggg tctggagggg tgcgaggagc ctcctttcct
     3721 ggttttgcgg cccttcgagg cgtcgacacc ggcaaaaatc gcggcagcgg gcgcagggct
     3781 ggctcgcggc gcttttgtcg caggcttggt ttcgccgtct tccggtcgtt tttttaaccg
     3841 gaacgctgct atcttccccg ctccttcacc cacacgcacg aaaacatgat cggtattgtc
     3901 attgtcacgc atgggcagct cgcggccgag cttttgtccg cggcccagca tatccaaggc
     3961 catcagtctc acgtgaagac cgtgtgcatc ggtcccaatg acgacatgga ggagcgccgc
     4021 gacgagatca ccgcggccat caaggcggtg gatctgggca gcggcgtcgt gctggtcacc
     4081 gatatgttcg gcggcacgcc ctgcaatctc gcgctcaccc atttgcagaa gggcaaggtc
     4141 gaagtgctcg cgggcgccaa tctgccgagc ctcatcaagc tcatcagtgt gcggatcacg
     4201 ctgccgctcg aaaaggcggt ctgtgaagcc atcgaatcgg gacgcaaata tatgcgcgcg
     4261 ggctcggcgg agcttgccgg ttcaccggga aaatgaatac cccacaaaca gtccgcacca
     4321 ttgcgaccat tgtgaacaag cgggggctgc atgcccgggc ctcggcgaaa ttcgtcgaga
     4381 ccgccgcgcg attccaatcc gaggtcaccg tgacaaaggg cgataccagc gtctctggcc
     4441 gttccatcat gggattgatg atgcttgggg cctcccttgg gaccgcgatc gaactcaccg
     4501 cggagggacc cgatgcgtcc tcggcggtgg gcgcgttgac aagcttgatc gctgccaaat
     4561 ttcacgaaga tgcctgaagg cagacgcggc gcttccgtgc taaatttctc tccacagcgg
     4621 gggaggatgc gcatgacagc agcgcagcaa ttgcggacca ggctcgccgg tgtcctttgt
     4681 gcctgcctga tatcggcgcc ggccgccgga ctcgcgcaag tggcacccca tccggccctt
     4741 tggcgcgtcg cgcgcggccc cgccacgatc tatctatttg gctctctgca tgtgctgccg
     4801 gcgggttatt cgtggagcac gccggaaatc gacgccgcca aggccgcgtc cgaaatgttc
     4861 atctttgagg tgccggtgga tgaggccgcg ctcaaggacg agagacaatt cattgtcgag
     4921 aatgggttgt tgggcagagg ccagtcgctg cgcggcgttc tcagcgcaaa cgagttccag
     4981 acctattccg ccgtgctcaa gcgtgcgggt ctgaaaccgc agcaattcga acgctatcgc
     5041 ccatggttgg ccgccgtcgt cgtgggcctg gcctatctgc acggcggcaa tctggattcg
     5101 cttcgggg
//
LOCUS       DASYEG010000128        11750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_5114726, whole genome shotgun sequence.
ACCESSION   DASYEG010000128 DASYEG010000000
VERSION     DASYEG010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 11750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11750
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5114726"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1616)
                     /locus_tag="VGM72_05415"
     CDS             complement(<1..1616)
                     /locus_tag="VGM72_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY4264735.1"
                     /translation="MNIKRAALLGSMALTLASLVTALPSSVRAAPAPDRIAIDNDDIG
                     GVVRNPSGQPEAGVWVIAETRDLAVRFIKIVVTDDQGRFVVPDLPQANYQVWARGYGL
                     VDSAKITSTPGKIVDIAAVPAPNDAAAAKYYPAIYWYSMMKIPGKELFGGKDPNIPAR
                     LTQQGYLNSMKNNGCVGCHQLGQLSTRTIPTLFHEEQSNYADMWLRRIQSGQGGMPMI
                     QVGAGDLGGAAFKYLGDWSQRVEKGEIPSNKPQRPQGVERNIVVTLRDWMNEKQYLHD
                     LISTDRRNPTVNGYGPLFGSPEYSSNDMPILDPVKNTATVFRMTVREDAPLALGPGHV
                     AGLTPLLPSNYWGEESIWDTRVNNHNSMIGRDGRVWLAATFRKPDNPAFCKAGSDHPS
                     AKVFPIARNVRSITVFNPKTKEYRWIDTCFGTHHLQFAYDKNDTLWASGGGPVVGWLN
                     TKMYDETGDAAKSQGWTPLILDTNGNGKRDAGWVEPNQPVDPTKDKRIVASFYAVMPN
                     PVDGSIWGTYRGNPGAIVRIEPGSNPSETAL"
     gene            complement(1789..2685)
                     /locus_tag="VGM72_05420"
     CDS             complement(1789..2685)
                     /locus_tag="VGM72_05420"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY4264736.1"
                     /translation="MSEHDRTDKFDEEAAVSRRAFGALSVGVGLAAVAAPARAASAPP
                     LTETDVAIKTPDGMCDAAYIRPATGAHAAVIIWPDAFGLRQSMRGMARRLAADGYSVL
                     VPNPFYRVAKAPVFPEPVKFDFTNPADTAKLGPLTGSIGAAGAAERDAVAFVAFLDAQ
                     KDVDKAKKIGTQGYCMGGALVVRTAAAVPDRIGAGGSFHGGNLVNDTPASPHLMAPKI
                     KARMYFGIAGNDDMRQPDQKTKLKEAFAAAHVPAEVEVYMSNHGWCVPDMPAAAGKPI
                     YNMAEAERAWGKLVALYKIGLA"
     gene            complement(2713..4896)
                     /locus_tag="VGM72_05425"
     CDS             complement(2713..4896)
                     /locus_tag="VGM72_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY4264737.1"
                     /translation="MNIKRAACAGMMSASFAALLCVTSANTAAAQGAAGQIHLDTNDI
                     GGVVAGAKGPEAGVWVIAETKDLPTKYVKIVVTDDQGRYVLPDLPKANYDIWVRGYGL
                     VDSPKVKAQPGKPLDLKAVTAPSRAAAAAYYPGIYWYSMLHIPDAKLFPGTGPQGNGM
                     PVTLKDQGQWLRYAKTDGCIHCHQIGDLATRTIPAQLGHFNTSAEAWERRIQSGQAGR
                     GMVEAIGLFDTGRQLSLLGDWTDRVAKGELPKTDPVRPTGVERAIVVTEWDWNTPKAY
                     VHDGISTDKRNPTVNANGLIYGSTEVSTELLPWVDPVHNTSGVLKTEYRDPKTPTTKT
                     DPSLAPSPYWGDEAIWDTHTNVHNPMFDDKGRLWLTARIRPAATQPAYCKAGSNHPSA
                     KAYPKDTSNRQTEVYDPKTGKISMIDLCFNTHHLNFDDNGTLWFSAGGPYDVIGWLDT
                     KKWDATHDDAASQGWSPFVIDTNGNGKRDAGWAGLKDPVDPTKDKQIMGAPYGINTAP
                     DGSVYGSILGFPGGVIRFDPKTQLSEYYEVPYKNAKTPMSGFSPRGMGITSDGVVWLA
                     LASGQAASFDRRLCKGPLNGPKAADAQNVCPEGWKLYQMPGPVFENLPKETVGATAEA
                     PYAMWVDQDNTLGFGKNIPIATASQSDALEALVDGKWVVMRIPYPMSYYAKGMDGRID
                     DAAAGWKGKGLFSTYSERAPTHIEGGKGQTSKVVHFQLRPSPLAD"
     gene            complement(5070..6020)
                     /locus_tag="VGM72_05430"
     CDS             complement(5070..6020)
                     /locus_tag="VGM72_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4264738.1"
                     /translation="MICNRFRAACFLAAAVAVSLVAVSGPGAADDTAPLALARDGYFY
                     VNARTTRVGGKTYVTDQMYVEERVPARRTHAYPIVMVHGGTMSGVNFTGTPDGREGWA
                     QYFVRQGYAVYVVDQPGRGRSGYLPASYGPARNVESTNSFARFVSQEKSKLWPQAVLH
                     TQWPGSGNPDDPAAQQVMASQLPAIESFEKQQFLNRDALIALFEKIGPGILMVHSQAG
                     AFAWPVADARPDLVRAILAIEPNGPPVHSVDFIGAPNWFKEGPVSNTYLQQAGIKPSV
                     IRLADLGIHGNSHVMMLEKNNQEVAAVIAGWLDKTLPKGK"
     gene            6111..6887
                     /locus_tag="VGM72_05435"
     CDS             6111..6887
                     /locus_tag="VGM72_05435"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY4264739.1"
                     /translation="MTYETLLVRTEGAVGIVTLNRPQALNALNSQLMDELTAAVDAFE
                     ADDAIRCVILTGSEKAFAAGADIGEMKSKSFAEVYKEDFITKNWERVSRARKPVIAAV
                     AGFALGGGCELAMMCDVILAADNAKFGQPEINLGIGPGAGGTQRLTRALGKAKAMEMC
                     LTGRMMDAAEAERAGLVAQVVPLADLMPEALKMAAKIAAASLPAMMMVKECVNRSFET
                     SLAEGLHFERRLFHAMFATQDQKEGMTAFMEKRKALFKDC"
     gene            7007..7273
                     /gene="rpsT"
                     /locus_tag="VGM72_05440"
     CDS             7007..7273
                     /gene="rpsT"
                     /locus_tag="VGM72_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY4264740.1"
                     /translation="MPSHPSAEKRVRQTARRTAVNKNRKSLIRTAVRKVEEALASGDK
                     AAATAALKLAEPAIMRGVTKGVIHKNTGSRKVSRLNARLKTLKA"
     gene            7766..8230
                     /locus_tag="VGM72_05445"
     CDS             7766..8230
                     /locus_tag="VGM72_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY4264741.1"
                     /translation="MSNRDSSEAVPSSAGYAGDLDPKESWERLSRNTAAQLIDVRTIP
                     EWNFVGVPDLGPLQRQVLFCEWQHFPQGANDTFVRQAAEALERTSYRPDVPLFFLCRS
                     GARSRAAAIAMTAAGYGPCFNITDGFEGGLDTERHRGRSGGWKACGLPWIQT"
     gene            8282..9796
                     /gene="dnaA"
                     /locus_tag="VGM72_05450"
     CDS             8282..9796
                     /gene="dnaA"
                     /locus_tag="VGM72_05450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEY4264742.1"
                     /translation="MNRPDGLPAKTGYQGMGKMGFPLQQTPEPAWSDLWGMVRDRLRL
                     EFGQGIFDTWIAPLSFAGVERGHVRLSAPSRLVRDYVASHHAAKLERAFAAASADFAS
                     LDIVIGFSDTRTGLAHKAVAPAPAPRANPLPASATGSGRAMGNVPGASLQGLWDRQPD
                     PAQSFASFVTGPSNEFAFKAAQRFAENGESEMGLLFIHGGFGFGKTHLLNAIALEART
                     VRGARVLFLRAEDFMRRFLAALRNQETLAFKEELRAADILLIDDLQHICGRTATISEF
                     LHTVNAFTDLKRKVVIAADRPPSGLEGLSDDIRSRLQGAVAIALEKPDAATRFAILKA
                     KAAALEKKLPRATIPESVLEQVATELNVSPRELLGVLMKLATYADLTGKPVTAEVAEE
                     AIGSGSRAATGDHRVTIDEIQKKTAEYYKLDLRELHSARRARRVARPRQVAMYLAREL
                     TSRSLPDIGRRFGGRDHTTVLHACRRIEALCKTDPVFQQEVDFLRKVLGRQPRS"
     gene            10034..11155
                     /gene="dnaN"
                     /locus_tag="VGM72_05455"
     CDS             10034..11155
                     /gene="dnaN"
                     /locus_tag="VGM72_05455"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEY4264743.1"
                     /translation="MKINVERGAFLKALGHVQSVVERRNTIPILSNVLIEAAKGRLKL
                     TATDLDIEIVESIPADVLRNGAATAPAHMLYDIVRKMPDGAQVQAELIAGDSGRLLVS
                     AGSIRFELACLPQEDFPQMSAGALPHRFRLGASDLVQLIEKTRFAISTEETRFYLNGI
                     YLHAAKDGKQPCLRAVATDGHRLARYELDLPEGAAEIPGVIVPRKTVAELRRLLEDGD
                     SAIEIGLSDTKVQFTFGSVELTSKLIDGSFPDYQRVIPTANDKVLTLDAKDFSQAVDR
                     VSTISADKTRAVKLNLDTDRVTLSVVNPESGTATEGLGANYAADAIEIGFNARYLLDI
                     TGQIVGKDVRFLLADAGSPTLIEDTEDGRTLYVLMPMRV"
     gene            11165..>11750
                     /locus_tag="VGM72_05460"
     CDS             11165..>11750
                     /locus_tag="VGM72_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264744.1"
                     /translation="MTQTARLMDDADQRPGKPIADETSLAGGAAALAVTQLELTNFRS
                     YARGRLHTGALPIVLAGANGTGKTNLLEALSLLSPGRGLRGAKLAAIQRKAPADTMSR
                     TDVFADSLWAVSAAIARPDGHWEIGTGLLPGNPNAPPRRALHLNAATAESADLAELIP
                     MLWLTPAMDRLFLEGASERRRFLDRLVFALDSTHA"
BASE COUNT         2174 a         3660 c         3625 g         2291 t
ORIGIN      
        1 agcgcggttt cgctcgggtt cgaacccggc tcgatccgga ctatcgcgcc gggattgccg
       61 cggtaggtgc cccagatcga accatcgacg ggattgggca tcaccgcata gaagctggcg
      121 acgatgcgct tgtccttcgt cgggtcgacc ggctggttcg gttcgaccca gccggcatcg
      181 cgtttgccat tgccgttggt atcgaggatg agcggggtcc agccttgcga cttcgccgca
      241 tcgccggtct cgtcatacat cttcgtgttc agccagccca ccaccggtcc gccgccgctc
      301 gcccacagcg tgtcgttctt gtcataggca aattgcagat gatgcgtgcc gaagcaggtg
      361 tcgatccatc tgtattcctt ggtcttcgga ttgaagacgg tgatggagcg cacattgcga
      421 gcgatcggaa acactttggc ggacggatga tccgagcccg ccttgcagaa ggccggattg
      481 tcgggcttgc ggaaggtcgc cgcgagccag acgcgcccgt cgcgcccgat catggaattg
      541 tggttgttga cgcgggtgtc ccagatcgat tcttcgcccc aatagttgga cggcagaagc
      601 ggtgtcaaac cggcgacatg gccggggccg agcgcgaggg gcgcatcctc gcgcaccgtc
      661 attctgaaga cggtcgcggt gttcttcacc ggatcgagaa tgggcatgtc gttggagctg
      721 tattccggcg aaccgaacag cgggccatag ccgttcacgg ttggattgcg ccgatccgtc
      781 gagatcagat cgtgcagata ttgtttctcg ttcatccagt cacgaagtgt gacgacgatg
      841 ttgcgctcca ccccttgcgg acgctgcggc ttgttcgacg ggatttcgcc cttctccaca
      901 cgctggctcc aatcgccgag atatttgaag gcggcaccgc cgaggtcccc ggcgccgacc
      961 tggatcatcg gcatgccgcc ctggccggac tgaatccggc gcagccacat atcggcataa
     1021 ttggattgct cttcgtgaaa cagggtcggg atggtgcgcg tggaaagctg gcccaattgg
     1081 tgacagccca cacagccatt gttcttcatg ctgttgaggt aaccctgctg ggtcaggcgc
     1141 gccggaatgt tcgggtcctt gccgccgaac agctccttgc cggggatctt catcatcgaa
     1201 taccagtaga tcgccggata atatttcgca gccgcagcgt cgttcggcgc cggcacggcc
     1261 gcgatatcca cgatcttgcc cggcgtgctc gtgatctttg ccgaatcgac caggccatag
     1321 ccccgcgccc acacctgata attcgcctgc ggcagatcgg gcacgacaaa gcgtccctgg
     1381 tcgtcggtga ccacgatctt gatgaagcgc accgcgagat cgcgggtttc ggcgatcacc
     1441 cagacgccgg cttccggctg gccgctcgga ttgcgcacga ccccgccaat gtcgtcattg
     1501 tcgatggcga tgcgatccgg agcgggcgcg gcgcgcaccg atgacgggag ggccgtaaca
     1561 agactggcca aggtcagggc catgctgccg agcaaagcgg cccgcttgat attcatgatg
     1621 ctgatccccc ttgtgacgcg caagaagcgc cgattggcca catcatagcc gtgccttgct
     1681 ggccataaaa gccaaccgcg tcccctcaac ggcaatttga ggcgcgcgcg tccgtcacag
     1741 agcgggataa cctcttcctc taggttggag ggccttctct tccggtaatc aggcgaggcc
     1801 aattttataa agcgcgacca gtttgcccca agcgcgttcg gcttcggcca tattgtagat
     1861 cggcttgccg gccgccgccg gcatgtccgg cacgcaccag ccatgattgc tcatatagac
     1921 ttcgacttcg gccgggacgt gggccgcggc gaaggcctcc ttcaatttgg tcttctgatc
     1981 gggctgacgc atgtcgtcat tgcccgcgat gccgaaatac atgcgcgcct tgatcttcgg
     2041 cgccatcagg tgcgggctcg ccggcgtgtc gttgacgaga ttgccgccgt ggaacgagcc
     2101 gcccgcgcca atgcggtccg gcactgccgc cgctgtcctg acaaccagcg caccgcccat
     2161 gcaatagccc tgcgtgccga tcttcttggc cttgtcgaca tccttctgcg catcgagaaa
     2221 cgcgacaaag gcaaccgcgt cgcgttcggc ggcgcccgcc gcaccgatgg atcccgtcag
     2281 cggccccagc ttcgccgtgt cggcgggatt ggtgaaatcg aatttcaccg gttccgggaa
     2341 taccggcgcc ttcgcgaccc gatagaacgg gttgggcaca agtaccgaat agccatcggc
     2401 cgcgagccgc cgggccatgc cgcgcatgga ctgacgcaac ccgaaagcat cgggccagat
     2461 gatcaccgcg gcatgagcgc ccgttgccgg gcggatatag gccgcgtcgc acatgccatc
     2521 cggggtcttg atggcgacat cggtttcggt caacggcggc gccgaagcgg ccctggcggg
     2581 ggccgcgaca gccgccaacc ccacgcccac ggacaaggcg ccaaaggcgc ggcgggagac
     2641 cgccgcttcc tcatcgaatt tgtccgttct gtcatgctcg ctcacgtgat cctccgtagg
     2701 ctgaacaatg gctcaatccg ccagcgggct cgggcgcaat tggaagtgta cgaccttgct
     2761 ggtctggccc ttgccgcctt cgatatgggt cggcgcacgt tccgaatagg tcgagaacag
     2821 gcctttgccc ttccatccgg ccgccgcgtc gtcgatgcgc ccgtccatcc ccttggcgta
     2881 gtagctcatg ggatagggaa tgcgcatgac cacccatttg ccgtccacca gggcttccag
     2941 cgcgtcggac tggctggcgg tcgcgatggg aatgttcttg ccgaagccca gcgtgttgtc
     3001 ctgatccacc cacatggcat agggcgcttc ggcggtggcg ccgaccgttt ccttgggaag
     3061 attctcgaag accggccccg gcatctgata gagcttccat ccttccgggc aaacattctg
     3121 tgcatcggcc gctttgggac cgttcagcgg ccccttgcac agcctgcggt cgaaggatgc
     3181 cgcctggccg ctcgcaagcg ccagccaaac gacgccgtcg ctcgtgatac ccatgccgcg
     3241 cgggctgaag cccgacatag gcgtcttcgc gttcttgtac ggcacctcgt aatattcgct
     3301 gagctgcgtc ttgggatcga aacggatcac gccgccggga aagcccagga tcgaaccata
     3361 gacgctgccg tccggagcgg tgttgatccc gtagggcgcg cccatgatct gcttgtcctt
     3421 cgtcggatcg acgggatcct tcagccccgc ccagccggca tcgcgcttgc cgttgccatt
     3481 ggtgtcgatg acgaagggcg accagccctg gctcgcggcg tcgtcatggg tggcgtccca
     3541 tttcttggtg tcgagccagc cgatcacgtc atagggtccg ccggcgctga accacagcgt
     3601 gccgttgtcg tcgaaattca gatgatgggt gttgaagcag aggtcgatca tgctgatctt
     3661 ccccgtcttc ggatcgtaga cttcggtttg acgattcgac gtatccttcg gataggcctt
     3721 cgccgaagga tggttcgatc ccgccttgca ataggccggc tgggtcgcgg ccggacggat
     3781 gcgagccgtc agccacaagc gtcctttgtc gtcgaacatc ggattgtgga cattggtatg
     3841 cgtgtcccag atggcctcgt ccccccaata gggcgaaggc gccagactcg gatcggtctt
     3901 ggtcgtcggc gtcttgggat cgcgatattc ggtcttgagc acgcccgatg tgttgtggac
     3961 cggatcgacc cagggcagca gctcggtact gacttccgtc gaaccgtaga tcagcccgtt
     4021 ggcattgacc gtgggattgc gcttgtcggt ggaaatcccg tcatggacgt aggccttggg
     4081 cgtattccaa tcccattccg tgaccacgat ggcgcgctcg acgccagtgg gccggaccgg
     4141 atcggtcttg ggcaattcgc ccttcgccac gcggtcggtc caatcgccga gcaaagagag
     4201 ctggcgtccg gtgtcgaaca gcccgatcgc ttcgaccatg ccgcggccgg cctggcctga
     4261 ttggatgcgg cgctcccagg cttcggcgct ggtattgaaa tggccgagtt gggcggggat
     4321 cgtgcgcgtc gccagatcgc cgatctgatg gcaatggatg cagccgtcgg tcttggcata
     4381 gcgcagccac tggccctgat ccttcaacgt caccggcatg ccgttgccct gtgggccggt
     4441 tccggggaac agcttggcat cggggatatg cagcatggaa taccaataga tgccgggata
     4501 ataggccgcc gcagccgcgc ggctcggcgc tgtcacggcc ttgagatcga gcggcttacc
     4561 cggctgcgct ttcaccttgg gggaatcgac aagaccgtaa ccgcggaccc agatgtcgta
     4621 attggctttg ggaagatcgg gcaggacgta gcggccctga tcgtccgtga cgacaatctt
     4681 gacgtatttg gtcggaagat ccttggtctc cgcaatcacc cagacgccgg cttcgggacc
     4741 tttcgcaccg gcgaccaccc cgccgatatc attggtatcg agatggatct gaccggcggc
     4801 gccctgtgcc gccgcggtgt tcgcggatgt gacgcacaac agcgcggcaa aactggcgct
     4861 catcataccg gcgcaggctg ctctcttgat gttcatcgtc ccctcgtggc gtttctatgt
     4921 ttggatgggc aagcggctgt aaccccgccg aacatagccc aggacgggtg cgagacctac
     4981 ggcaattcag aggttggaac ccaagttttc acacggcagc ggcaaatttt gccaggcttt
     5041 tcgcaacgtc cggcgaggaa cgtcttccgt cacttgcctt tcggcagggt cttgtcgagc
     5101 cagccggcaa tcaccgcggc gacctcctga ttgttcttct ccagcatcat cacgtgcgaa
     5161 ttgccatgga tgccgagatc ggcgaggcgg atgacggagg gtttgattcc cgcctgctgc
     5221 agataggtgt tcgacaccgg tccttccttg aaccagttgg gggcgccgat gaagtccacg
     5281 ctgtgtacgg gcggcccgtt cggctcgatg gcaaggatgg cgcggacaag atcggggcgc
     5341 gcatcggcga caggccaggc aaaggcgccc gcctgcgaat gcaccatcaa gatgcccgga
     5401 ccgattttct cgaacagcgc gatcagcgca tcgcgattga ggaattgctg cttctcgaaa
     5461 ctttcgatgg ccggcaattg actcgccatc acttgctgcg cggcgggatc gtccggattg
     5521 ccgctgcccg gccattgcgt gtgcaacacg gcttgcggcc agagtttgga tttttcctgg
     5581 gacacgaaac gcgcgaagga attcgtgctc tccacattgc gcgccggtcc ataactcgcc
     5641 ggcagatagc ccgaacgccc ccgtcccggc tgatccacca catagacggc ataaccctgc
     5701 cgcacgaaat attgcgccca gccttcgcgg ccatcgggcg tgccggtgaa attcaccccc
     5761 gacatggtgc cgccgtgcac catcacgatg ggataggcat gggtgcggcg cgccgggacc
     5821 cgttcttcga catacatctg atcggtgaca taggtcttgc cgccaacacg cgtcgtcctg
     5881 gcgttgacgt agaaatagcc gtcgcgcgca agcgcgagcg gcgcagtgtc atccgcagcg
     5941 ccaggccctg aaacggcgac aaggctcacg gcaacggcgg cggcgagaaa acaagcggct
     6001 ctgaaacgat tgcagatcat aagcgtcctc ccgatcgccc atcatgccat gttctcttgc
     6061 cgtgcgcgcc ttcctgagag catattgcag cgcaacatgg cgggtacatc atgacttacg
     6121 agacacttct ggtccggacc gaaggcgcgg tcggcattgt cacgctgaac cggccgcagg
     6181 cgctgaacgc gctcaactct caattgatgg acgaattgac ggcggcggtc gacgccttcg
     6241 aggccgacga tgccattcgc tgcgtcatcc tcacgggctc ggaaaaagcc ttcgcggcgg
     6301 gtgccgacat cggcgagatg aaatccaaga gctttgcgga ggtctataag gaagacttca
     6361 tcaccaagaa ttgggagcgc gtgtcgcgcg cgcgcaaacc ggtgattgcg gcggtcgccg
     6421 gcttcgcgct cgggggggga tgcgagcttg ccatgatgtg cgatgtcatc ctggccgcgg
     6481 acaacgccaa attcggtcag ccggagatca atctcggcat cggccccggt gccggcggca
     6541 cgcagcgcct gacgcgcgcg ctcggcaagg ccaaggccat ggaaatgtgc ctcacggggc
     6601 ggatgatgga cgcggccgaa gccgaacgcg ccgggctggt ggcccaggtg gtgccattgg
     6661 ccgatttgat gcccgaagcc ctgaaaatgg ccgcgaagat cgcggcggcc tcgctgccgg
     6721 ccatgatgat ggtgaaggag tgcgtgaacc gcagtttcga aaccagcttg gccgaagggc
     6781 tgcatttcga gcgccgcctg ttccacgcga tgttcgcgac gcaagatcag aaggagggca
     6841 tgacggcctt catggaaaag cgcaaagccc tcttcaagga ctgctgatct ttcgggcgcg
     6901 tgtgggcgcg ctccttaaga ataagcgcat aggcggttcg cattgacgct gcggcgccgg
     6961 gactgtaatg gtccgcccct taaatccatc gagcaagagc gagcccatgc ccagccatcc
     7021 ttcggccgaa aaacgcgtcc gccagaccgc ccgccgcacc gccgtcaaca agaaccggaa
     7081 aagcctgatc cgcaccgccg tgcgcaaggt cgaggaggcg ctagcctccg gcgacaaggc
     7141 ggccgccacg gccgcgttga agcttgccga acccgcaatc atgcgcggcg tgacaaaggg
     7201 cgtcattcat aagaacacgg gttcccgcaa ggtttcccgt ttgaacgccc ggttgaaaac
     7261 cctcaaggcc tagcttttcc gcggctttgc aatattcgaa aataattttc gcgaataaag
     7321 ggtgaacatt tcatttgtca gtgggcggac cttccaatcg tctgtgaacg tcactgtcgt
     7381 ctaactttca cagatccgcc gcctaactcc ctgaatccgc tggcatcaaa tagtccgtac
     7441 actccgcagg gttcatccgt acacgtcaag tccctcattt tgatgcactt ttttcgttat
     7501 gacggaagcg tcacaggacc attcacaatc taaaaatggg ccttgcgttg gtaaatcacc
     7561 tctgtgtaga gttgccttca tcggcggggc ggcaaacatg acgcggcgaa gtttcgggat
     7621 aaatcccaat aaaaagaaga ctttacgtcc cgatgtgcgg cagccttcca aaagtcgcta
     7681 agtaaaacca agtatttgta gagtttcatt tgacgaagtg ctttcagcac tttcgcggcg
     7741 gctgtttatt gtccggagca ttggtatgag caatcgggat tcatccgagg cggttccatc
     7801 gtccgccgga tatgcggggg atttggaccc gaaggaaagc tgggaacggc tttcccggaa
     7861 taccgcggcc cagctcatcg acgtgcgcac aattccggag tggaactttg tgggagttcc
     7921 cgacctcgga ccgttgcagc ggcaggttct gttctgcgaa tggcagcact ttccacaggg
     7981 cgccaacgac accttcgtcc gtcaggcggc ggaagccctg gaacgcacct cctatcggcc
     8041 ggatgtgccg ctcttcttcc tctgccgcag tggcgcacgc tcgcgcgccg ctgcgatcgc
     8101 gatgacggcg gcgggctatg gcccctgctt caacattact gacgggttcg aaggcggcct
     8161 cgatacggag cggcatcgcg gaagatcggg cggctggaaa gcctgcgggc ttccctggat
     8221 tcaaacatga acggcagggc gcgcgaacgc caaaacgacg agatgggatt tgggtggaac
     8281 gatgaataga cccgatggac taccggccaa gaccgggtat cagggaatgg gcaagatggg
     8341 atttccattg cagcagacac cagagccggc atggtccgat ctgtggggta tggtgcgcga
     8401 ccggcttcgc ctcgaattcg gacagggcat tttcgacact tggatcgcgc ctctttcttt
     8461 tgcgggcgtc gaacgcggac atgtgcgtct ttcggcgccg agccgtctgg tgcgcgatta
     8521 tgttgcgtcc catcacgccg cgaagctgga gcgcgccttc gccgcggcca gcgcggattt
     8581 cgccagcctc gatatcgtca tcggattttc ggacaccaga acgggccttg cccacaaggc
     8641 cgttgcgccc gcgccagccc cgcgcgccaa ccccttgccc gcatccgcca ccggatcggg
     8701 ccgcgcgatg ggcaatgtgc cgggtgcctc gctgcaaggc ctttgggacc gccagccgga
     8761 cccggcccag agctttgcat cgttcgtgac cggtccttcc aatgaattcg ccttcaaggc
     8821 ggctcagcgc tttgccgaga acggcgaatc cgaaatgggg ttgctgttca tccatggcgg
     8881 ctttggcttc ggcaagacgc atttgttgaa cgcgatcgcg ctggaagcgc gcacggtgcg
     8941 gggcgcgcgc gtgctgttcc tgcgcgcaga agatttcatg cgccgtttcc tcgcggctct
     9001 gcgcaatcag gagacgctgg cgttcaagga ggagcttcgc gccgccgaca tccttctgat
     9061 cgacgatctg cagcatattt gcgggcggac cgcgaccatc agcgaattcc tgcacacggt
     9121 gaacgccttc accgatctga agcgcaaagt cgtcatcgcg gccgatcgtc cgccttcagg
     9181 gttggaaggg ctttccgacg atatccgctc gcgtctgcaa ggggcggtcg ccatcgcgct
     9241 cgaaaaaccc gacgccgcga cacgtttcgc gatcctcaag gcgaaagccg cggcgcttga
     9301 aaaaaagctt ccgcgcgcca ctattcccga atctgtgctg gagcaggtcg ccaccgaact
     9361 gaatgtgtct ccgcgcgaac ttctgggcgt gctgatgaag ctcgccacct atgccgatct
     9421 cacgggcaag ccggtgaccg ccgaagtcgc cgaagaagcc atcggcagcg gcagccgcgc
     9481 ggcgaccggc gatcaccgcg tcaccatcga cgagattcaa aagaaaaccg ccgaatatta
     9541 caaattggac ctgcgggaat tgcattcagc gcggcgcgcg cggcgcgtgg ccaggccccg
     9601 ccaagtggcg atgtacctcg cgcgcgaact caccagccgg tcgcttcccg acatcggccg
     9661 ccgtttcggg ggacgcgatc ataccacggt gctccatgcc tgccggcgca tcgaggccct
     9721 gtgcaaaacc gatccggtat tccagcagga ggtcgatttc ctgcgcaagg ttctcggccg
     9781 tcagccccgc agctgaagca gcttgaaaag cctcatcccg cgcatcccgg cggatggggc
     9841 ttcggccccc ggtctccaag cccgctcccc tcgattttcg cacgcccggg cggctcctgt
     9901 tcgagtcgct tgttcgcgcg attgtagcta tgcttcgcgc gcaagaatcg tgggcccgga
     9961 tgggcaataa tttggcggcc aaattggata gtttcgatcg ttttcctaaa aaaatcctgg
    10021 aaccccaaga accatgaaaa tcaatgtcga acgcggcgct ttcctgaagg cgcttgggca
    10081 cgtccaaagc gtcgtggaac gccgcaatac gatccccatc ctctcgaatg tgctgatcga
    10141 ggcggccaag gggcggctga aactgacggc caccgacctc gatatcgaga tcgtggaatc
    10201 gatccccgcc gacgtgctgc gcaacggcgc ggcaaccgct cctgcccata tgctctacga
    10261 tatcgtgcgc aagatgcccg acggagccca ggtgcaggcg gagctgatcg cgggcgattc
    10321 ggggcggctg ctggtgtcgg cgggaagcat ccgcttcgag ctggcctgtc tgccgcagga
    10381 ggatttcccg caaatgtcgg ccggcgcgct gccgcaccgt ttccggctgg gggcctcgga
    10441 cctggtgcag ctgatcgaaa agacccgctt cgccatttcg acggaggaga cgcgtttcta
    10501 tctcaacggc atctatctgc acgcggccaa ggacggcaaa cagccatgcc tcagagccgt
    10561 tgcgaccgat ggccaccggc tggcccgcta tgagctggat ttgccggagg gcgccgccga
    10621 aattcccggc gtaatcgtgc cgcgcaagac ggtcgccgaa ttgcgccggc tgctcgagga
    10681 tggcgacagc gccatcgaaa tcggtctttc cgataccaag gtgcagttca ccttcggcag
    10741 cgtggaattg acctcgaaac tgatcgatgg ctcgttcccg gattatcagc gggtcatccc
    10801 gaccgcaaac gacaaggtgt tgacgctcga tgccaaggat ttttcccagg cggtggaccg
    10861 cgtctccacc atttccgcgg acaagacccg ggcggtgaag ctcaatctcg ataccgaccg
    10921 ggtgaccttg tcggtggtca atcccgaatc cggcacagcc accgaaggat tgggcgcgaa
    10981 ttatgccgcc gatgctattg agatcggctt caacgctcgc tatctcctgg acatcaccgg
    11041 gcagattgtc ggcaaggatg tgcgctttct tcttgcagat gccggctcac ccacgctcat
    11101 cgaagacacc gaggacgggc gcacgcttta cgtcctgatg ccgatgcggg tttaggaatt
    11161 tgaattgacg cagacagcca ggcttatgga tgacgccgac cagcgtccgg gcaagcccat
    11221 tgcggacgag acatcgcttg cgggcggcgc ggccgcgctc gcggtcacgc agctggagct
    11281 gaccaatttt cgctcctatg cccgcgggcg gctacacacg ggggcgttgc caatcgtgct
    11341 tgcgggcgcg aacgggaccg gcaaaaccaa ccttctggaa gcgctctccc tgctgtcgcc
    11401 cggacgcggt ttgcgcgggg cgaaactcgc cgccatccag cgcaaggcac ccgccgatac
    11461 aatgtcgaga acggatgttt ttgccgacag cctctgggcg gtgtcagcgg ccatcgcgcg
    11521 tcccgacgga cattgggaga tcggcaccgg actgctgcca ggcaatccga atgcgccgcc
    11581 gcgccgtgcg ctgcacctga atgctgcgac ggccgaaagc gccgatctgg ccgaactcat
    11641 tccgatgctg tggctcacgc ctgcgatgga ccggctattc ctggaaggcg catccgaacg
    11701 ccgccgtttt ctcgaccgtt tggtctttgc gctcgattcc acgcatgcca
//
LOCUS       DASYEG010000129         9825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4966061, whole genome shotgun sequence.
ACCESSION   DASYEG010000129 DASYEG010000000
VERSION     DASYEG010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 9825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9825
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4966061"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            508..1395
                     /locus_tag="VGM72_05465"
     CDS             508..1395
                     /locus_tag="VGM72_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HEY4264745.1"
                     /translation="MTAKIIDGLAIAREVRVGIAARAQRLAERGITPALTMIRVGDDP
                     ASGVYVRNKVRACADVGIRSDVVRLPADVSEDVLLDRIRKLNEMSFVHGILVQLPLPA
                     HIAPRRVLETISVAKDVDGFHLYNVGGLVIGQTVFPPCTPYGVQKILEHENIELEGKN
                     VTVVGASNIVGKPLALMLMQREATVAICHAKTRDLAQYTILADILVVAAGKPNLIVAP
                     MVRHGAVVIDVGINFLPGGKLVGDVDFEGVKEKAACITPVPGGVGPMTVTMLLENTVG
                     AAERAALSMAAVHVRPVPV"
     gene            1518..2750
                     /locus_tag="VGM72_05470"
     CDS             1518..2750
                     /locus_tag="VGM72_05470"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEY4264746.1"
                     /translation="MTLPRLSLFLLGASVLGASVLSLPLPSAAAPAPAIQTVPGMPPV
                     VDTQNVYSEAGINKVSAVTAGALARVYVPNVKSGDVYVIDPATYSVVDHFKVGGNPQH
                     IIPSWDLKTLWVAGSAERKLPGILTPIDPKTGKPGTTINVPDAYNLYFMPDGSSAIVV
                     AEALKRLEFRDPQTMKLQSNLATPQCAGLNHADFSIDGRYAIFTCEFAGGGLAKIDMV
                     AKKVVGYLKLTKQGLPGMPAMNMPGANNAAASMPQDIRIAPDGKLFYVADMQADGVYL
                     VDGDSFQEVGFIPTGRGTHGLYPSRDGKKLYVTNRGTHMGDVKAHGPGMISVIDFATR
                     KVEKTWSIPGGGSPDMGNVSADGKTLWLSGRFDKVVYAIDTTSGAVKEIPVGTEPHGL
                     TVWPQPGRYSLGHTGNMR"
     gene            complement(2756..3673)
                     /gene="copD"
                     /locus_tag="VGM72_05475"
     CDS             complement(2756..3673)
                     /gene="copD"
                     /locus_tag="VGM72_05475"
                     /inference="COORDINATES: protein motif:HMM:NF033808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HEY4264747.1"
                     /translation="MTVAAGLIASRFVHYAALMILFGVAAFPVYAYYAKAPSAFEAEA
                     FFPWSRRVQIVSVLAALVSGILWLGFVAATMSGQPAALFDANILWQVISTTDFGRVWA
                     PRLGLTGVALILLLPKNPPETLRLTVLGCAAIALTSIADTGHAGADNGPRAALHITAD
                     AVHLSAAGVWVGALFILARFALIARRLEDRDLRIFDHALTRFSGVGPVVVGALTLSGI
                     LNPGFLASLETTYGQVLLAKLVLFGAMLLLAAANRFRLAPRLQFALASGIGSESATRA
                     LRTSVLAETVLSLLVLAAVAWLGTLAPPA"
     gene            complement(3670..4038)
                     /gene="copC"
                     /locus_tag="VGM72_05480"
     CDS             complement(3670..4038)
                     /gene="copC"
                     /locus_tag="VGM72_05480"
                     /inference="COORDINATES: protein motif:HMM:NF033814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis periplasmic binding protein
                     CopC"
                     /protein_id="HEY4264748.1"
                     /translation="MTIVKTVMLALAATVGMISFAGAHAQLKAAGPAPGSVVGTSPKM
                     LRIQFNEAIEIGFSGVEVTNAKGEKVPTGAATTAANDKAQLIVPLTGELAPGKYTVAW
                     HAVGDDTHRTEGRYNFEVKQ"
     gene            complement(4070..5299)
                     /locus_tag="VGM72_05485"
     CDS             complement(4070..5299)
                     /locus_tag="VGM72_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264749.1"
                     /translation="MEPHNHDRSPEDDLPPLGTGPSVQATGVLGDYSMMRDSSGTSWQ
                     PDTTPMEGVHGLLGDWSTMVHGYISAIYDHQGGPRGGDKSFSESMLMGMAQHSLGPGH
                     LTLRGMISLDPLMGKNGYPLLLQTGETADGSSPLIDRQHPHDLFMELSGTYSAPVADS
                     TSAFLYLGYPGEPALGPPTFMHRFSGMDDPEAPIGHHWLDSTHITYGVATAGIVSGEW
                     KFEGSIFNGRDPDQERWDFDPLRLDSVSGRISWNPNPDWAFQASYGFIKSPEQLEPAI
                     NQHRITASAIYNRKLAHGNWQTTFAWGQNIQQPGTASNAYLLESALRWYAHTIFARAE
                     NDEKDELFAAPSPLEGHAFNVSKFSLGYIYDIAVAEHLSLGLGAVGSLYGLPSAVKPY
                     YGSNPGSIMLFARVKLT"
     gene            5522..7183
                     /locus_tag="VGM72_05490"
     CDS             5522..7183
                     /locus_tag="VGM72_05490"
                     /inference="COORDINATES: protein motif:HMM:NF011291.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEY4264750.1"
                     /translation="MAAEAPTREAGRDFIRDIIQSDLDSGRVTSVVTRFPPEPNGYLH
                     IGHAKSICLNFGVAQEFGGRCHLRFDDTNPAKEEQEYIDAIKRDVAWLGFDWGEHLHH
                     ASDYFEQLYEWAEHLIGSGKAYVDDQPHEEMRANRGTLTEPGRNSPYRDRTVEENLAL
                     FRRMRAGEFPNGARVLRAKIDMASGNINLRDPVLYRILNAIHPRTGDAWKIYPSYDFA
                     HGQSDAIEHITHSLCTLEFEDHRPLYDWFLDNLPVPSRPHQYEFARLNIANTLLSKRT
                     LTELVRGGHVSGWDDPRMPTLAGLRRRGVPPEAVRDFVRRAGVAKANSVVDAAAFDYA
                     VREVLNRRAPRRMAVLNPLKIVIENYPEGQTEELEAINNPEDPSAGSRRIPFGRELYV
                     EREDFMEDPPKKFFRLSPGREVRLRYAYFITCREVVKNAAGEVAELRCTYDPATKGGN
                     APDDRKVKATLHWVAAASALPAEIRIFNPLFTRPDPGADGDFAQDLNPNSLEILPGAQ
                     LEPALGDAREGEAIQFERQGYFCLDAGPSRNRPVFNRTVSLRDSK"
     gene            7200..8738
                     /locus_tag="VGM72_05495"
     CDS             7200..8738
                     /locus_tag="VGM72_05495"
                     /inference="COORDINATES: protein motif:HMM:NF005760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEY4264751.1"
                     /translation="MNGAESLVRTLLASGIDTCFANPGTSEMHFVAALDRAPGMRCVL
                     GLFEGVVTGAADGYGRMAGKPAATLMHCGPGLANGLAYLHNAKRASTPIVNIVGDQAT
                     YHAPLNPPLASDVEGIARPVSQWVRKVTQTATLGADAAEAVRSAYQPPGGVATLILPS
                     DVSWNDGGAPGTPLPIPAPANVANDAVIEAASALRRGERTVILLAGAALHAAALAIAH
                     NIAEATGARIMAPTFNARIARGAGRPLLDIVPYPVDEAVAQLKDVKHLILVGTSEPVA
                     FFAYPGKPGIMSPKDAVSHVLARADEDLRDALTRLASELNCPNTPIPEYRANALPGAG
                     PMSAEGAGLTLAALLPEQAILIDEAITLRGPFFAATKTAAPHDWLQVPGGAIGGGLPL
                     ATGAAIGAPGRRVIAIQADGSAMYTIQSLWTQARENLDVTTLLLSNRKYAILFHELKQ
                     VGATEGRASSDLFALDRPELDWIKLSESMGVEAARAETLERFAALLTHANGRRGPFLI
                     ELVL"
     gene            8787..9752
                     /locus_tag="VGM72_05500"
     CDS             8787..9752
                     /locus_tag="VGM72_05500"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEY4264752.1"
                     /translation="MTISTLKRIGIGGAVLALGLGGIDAFAAAPKLDGTVKQESVAVK
                     GAQRTFVLYVPHDLKPNAPLLFVFHGSGGDGASMRDVTAFEFDMLADRDGFLVAYPDG
                     YQTTWNDCRKGSPQPARRMNIDDESFVDAMIAKEAAEHAIDRKRVFSAGWSNGGQLGY
                     RFAMERANVFTGVAALSATVPVPANLACTPSGAAMPMMIINGTADPINPFGGGNVMLG
                     GSNLGAVYSSQDGAQYWAKLNGITSGPQTKRLPHRNASDPTYVDEMTWSSAGKPLVVM
                     YAVHGGGHVVPTRVPQDWGGLGRETGDLDAPVAIWDFFSKLPSRP"
BASE COUNT         1899 a         2985 c         3055 g         1886 t
ORIGIN      
        1 tctcggaaca gatcctggcg cgaaccccac gcccgcaacg cagccgcgcg cgaagggacc
       61 gccacaatcg tgtcatagaa gccgaggtgg gcgtggaatg ttttcagcgc gcgcggcatg
      121 taaggtttat atcagagctt cgaaggaagg tgggagccta aggatgcaat ggatccggcg
      181 agcgatgcga atgccaattg gccggacacg atctaagcgc atttctgctg cgccgggatt
      241 cgtcaggtgg gctacgtgcc cgatacgggg catagccacc cattccggga tcgctcttgc
      301 ggcaatcgcc aaagcttgcc gcttcacgca agtgtgctcg atttgcacgc tggaaaagct
      361 ggacgcggcg cacgctctgc ttcattccgg cgccggtccg gtcttcattc atgcgcaggt
      421 caaggctgag gatttgccgc gtgtgctgcc gaaccgggat ggaaacgcga taaaactccg
      481 cttcatgcag acgttggcac aatcatcatg acggcaaaga tcatcgacgg tctggcgatc
      541 gcgcgcgaag ttcgcgttgg gattgcggca cgcgcgcaaa gactggcgga gcgagggatc
      601 acacctgcct tgacgatgat ccgggtgggc gatgatccgg cctccggcgt ttatgtgcgc
      661 aacaaggtgc gcgcctgcgc cgatgtcggg atcagatcgg acgtggtgcg tctacctgcg
      721 gacgtcagcg aggacgtgct tctcgaccgc atccggaaat tgaacgagat gtctttcgtt
      781 cacgggattc tggtgcagct ccctttgccc gcccatatcg cgccgcgccg cgtactggaa
      841 accatatcgg tcgccaagga tgtggatgga tttcatctct acaatgtcgg cggcctggtg
      901 atcggccaga cggttttccc gccctgtacc ccttatggcg tgcagaaaat cctggaacac
      961 gaaaatatcg agctggaagg caagaacgtc accgttgtcg gcgcgagcaa tatcgtgggc
     1021 aagcctttgg cgctgatgct gatgcagcgc gaggcgaccg tcgccatttg ccatgccaag
     1081 acgcgcgacc tcgcccagta cacgatcctt gccgatatcc tggtggtggc ggcgggcaag
     1141 ccaaatctca tcgtggcgcc gatggtgcgg catggcgccg tggtcatcga tgtcgggatc
     1201 aattttcttc ccggtggcaa gcttgtcggt gatgtcgatt tcgagggcgt aaaggaaaaa
     1261 gcggcctgca tcacgccggt tcccggcggc gtcggcccga tgacggtcac aatgctgctc
     1321 gaaaacaccg tcggggcggc ggaacgcgcg gccctgtcta tggcggccgt gcacgtgcgc
     1381 cccgtaccgg tgtgacatta actgggtgtg ctgcgttctt tgccgcgtgg tacgatgttt
     1441 cgattctcgc ctgcggacct taaactcccg caggaaggtt gccgatcttg aatcctgacc
     1501 caattcggat aatcaccgtg acccttcctc gtctgtcgct gtttcttctt ggcgcttcgg
     1561 ttcttggcgc ttcggttttg agcctgccgc ttccgtcagc cgccgctccg gcaccggcga
     1621 ttcagaccgt cccgggcatg ccacccgtgg tggataccca aaatgtttac agcgaggcgg
     1681 gcatcaataa ggtgagcgcc gtcacggctg gcgcactggc gcgcgtttat gttcccaatg
     1741 ttaaatcggg cgatgtctat gtgatcgatc cggcgaccta ttcggtggtc gatcatttca
     1801 aggtcggcgg caatccgcag cacattattc cgtcctggga tctgaagacg ctgtgggttg
     1861 cgggttcggc cgaacgcaaa cttcccggca tattgacgcc tatagacccc aaaaccggca
     1921 agcccggcac aacgataaat gttcccgatg cctataacct ctatttcatg cctgacggtt
     1981 cctcggccat cgtggtcgcc gaagcgctga aacggctcga attccgcgat ccgcagacca
     2041 tgaaattgca atccaatctc gcaactccgc aatgcgcggg cctcaaccat gccgattttt
     2101 ccatcgacgg ccgctacgcc atcttcacct gtgaattcgc cggcgggggc cttgccaaga
     2161 tcgatatggt cgccaagaag gtcgtgggct atttgaagct gaccaagcag ggcctgcccg
     2221 gcatgccggc aatgaacatg ccgggcgcga acaacgccgc cgccagcatg ccgcaagaca
     2281 tccgcatcgc ccccgatggg aagctgttct acgtcgcgga catgcaggcc gatggagtct
     2341 atctggtcga tggcgattcg ttccaggagg tcggcttcat tccgaccggg cgcggcacgc
     2401 atggccttta tcccagccgc gacggcaaga agctctatgt caccaatcgc ggtacccata
     2461 tgggcgatgt gaaggcgcac ggccccggca tgatttccgt gatcgatttc gccacccgca
     2521 aggtggagaa gacgtggtcc attcccggcg gcggcagtcc cgatatgggc aatgtcagcg
     2581 ccgatgggaa gacgctatgg ctctccgggc ggttcgacaa agtggtctat gcgatcgata
     2641 ccacgagcgg ggcggtgaag gagatccctg tcggcaccga accccacggc ctcactgtct
     2701 ggccgcagcc cggccgctat tccctcggcc acacggggaa catgcgctga gccttttatg
     2761 cgggcggcgc cagcgtgccg agccatgcga ccgcggccag caccagtagc gaaagcacgg
     2821 tttcggcaag cacgcttgtc cggagcgcgc gtgtcgcgct ttcggaaccg ataccggatg
     2881 cgagcgcgaa ctgaagacgc ggcgccaatc ggaagcggtt ggcggctgcc agcaggagca
     2941 tcgcaccgaa cagcacgagc ttggcgagca acacctgacc gtaggtcgtc tcaaggcttg
     3001 ccaggaaccc tggattgaga atgccgctca aggtcaacgc gccaaccacg accggtccca
     3061 cccccgaaaa acgcgtcagc gcgtggtcga agattctgag atcgcgatcc tcgagccgcc
     3121 gcgcgatcaa cgcaaaccgg gcgaggatga acagagcgcc gacccaaacc cccgccgcgg
     3181 aaagatgcac ggcatcggcg gtgatgtgca acgcggctct tggaccgttg tccgcgccgg
     3241 catgcccggt gtcggcgatg ctggtgagtg cgatggcggc gcatccgagc acggtcagac
     3301 gcaatgtttc cggtggattt ttgggcagca gcaatatcag ggcaacgccg gtcaggccga
     3361 ggcgtggcgc ccatacccgg ccgaaatcgg tggtgcttat cacctgccac aatatgttcg
     3421 cgtcgaacaa agcggcaggc tgtccactca tggtcgcggc aacaaagccg agccagagaa
     3481 tcccgctgac gagcgcggcg aggaccgaaa caatctgcac gcgccgcgac caggggaaaa
     3541 acgcttccgc ttcgaaggct gaaggcgcct ttgcgtaata ggcgtagacc ggaaaggccg
     3601 ccacgccgaa gagaatcatc aacgccgcat aatgcacgaa gcgcgaggcg atcagccccg
     3661 ctgctaccgt cattgcttca cttcgaaatt gtagcgtcct tccgtgcgat gcgtatcgtc
     3721 gccgaccgca tgccatgcca ccgtatattt tccgggggcg agttcgcccg tcagcggaac
     3781 gatgagctgc gccttgtcgt tcgcggcggt cgttgcggcg ccggttggca ctttctcgcc
     3841 cttggcgttg gtgacctcca ctccggagaa gccaatttcg atggcttcgt tgaactggat
     3901 gcgaagcatc ttgggcgatg tgcccacgac gcttcccggc gccggaccgg cagccttgag
     3961 ctgcgcatgc gcgccggcga acgaaatcat tccgaccgtg gcggcaagcg ccagcattac
     4021 tgtttttacg attgtcatat tgtgctccct ttggctcatc ttatacgcat taggtcagtt
     4081 ttacccttgc aaacagcatg atcgagccgg gattggagcc ataataaggc ttcaccgccg
     4141 agggcagtcc atagaggctg ccaacggccc caaggcccag gctcaaatgc tccgccactg
     4201 cgatgtcata gatatagccg aggctgaatt tcgacacgtt gaaggcgtgt ccttccaatg
     4261 ggctcggggc cgcgaacagt tcgtcttttt cgtcgttctc ggcccgggcg aagatcgtat
     4321 gggcatacca gcgtaacgcc gattcaagca gataggcatt cgatgccgtg cctggctgct
     4381 ggatgttttg accccaggcg aacgtggtct gccagtttcc atgcgcgagc ttcctgttgt
     4441 agatcgccga cgcggtgatc cggtgctggt tgatcgcggg ctccaattgc tcgggcgatt
     4501 tgatgaagcc atagctcgcc tgaaacgccc agtctggatt tggattccag ctaattcgtc
     4561 ccgagacgga atccagccgc aaagggtcga aatcccagcg ctcctggtcc ggatcgcggc
     4621 cattgaagat cgagccttcg aatttccact cgccagaaac gatgccggcg gtcgcgacgc
     4681 cataggtgat gtgtgtcgag tcgagccaat gatgaccgat cggtgcttcc ggatcgtcca
     4741 ttcccgaaaa gcggtgcatg aaggtcggcg gtccgagcgc cggttcgccg ggataaccca
     4801 gataaaggaa ggcggatgtg ctatccgcaa cgggtgcgct gtaggtgccg gagagttcca
     4861 tgaagagatc gtgcgggtgc tgccggtcga tcagggggga acttccgtca gcggtttccc
     4921 ccgtctgcag cagcagcgga taaccgttct tacccatcag cggatcgagc gagatcatac
     4981 cgcgcagggt tagatggccc ggcccgagac tatgctgggc catgcccatc agcatgctct
     5041 cgctgaagct tttgtccccg ccgcgcgggc ctccctggtg gtcgtagatg gccgagatgt
     5101 agccatggac catcgtggac caatctccga gaaggccgtg gacgccctcc atcggggtcg
     5161 tatccggttg ccaggaggta ccggagctat cgcgcatcat ggaatagtcc ccgagcacgc
     5221 cggtcgcctg aacgcttggc ccggtgccga gtggcggcag atcgtcttcg ggcgatctgt
     5281 cgtggttatg aggctccata ggttgcgcga ccgcaggaga tgacagcagg agcaaaatca
     5341 aagcggtctc gaaaaatctc agagcgcgaa tcccatttcg tataccccgc aggggtatgc
     5401 gcggaatatc atggacgaac atggactgca accgctcttg gcgggcaggc gtggatttgc
     5461 cacaacgtgc gcccggtgcg aaagcacctg cggaagaaac gtggaaatcg gagcggtgtt
     5521 gatggcagca gaagcgccga cgcgcgaggc aggtcgcgat ttcatccgcg acatcatcca
     5581 gtcggatttg gattccggcc gggtgacctc tgtcgtgacg cgctttccgc ctgagccgaa
     5641 tggctatctt catatcgggc acgccaagtc catctgcctc aatttcggtg tggcgcagga
     5701 atttggcggc cgctgtcatc tgcgtttcga tgacaccaat ccggccaagg aggagcagga
     5761 atacatcgac gccatcaaac gcgatgttgc ctggctcggt ttcgattggg gcgagcatct
     5821 gcatcatgcg tcggactatt tcgagcagct ttacgaatgg gctgagcatc tgatcggaag
     5881 cggcaaggcc tatgtcgacg accagcccca tgaagaaatg cgcgccaacc gcggcacgct
     5941 gacagaaccc ggcaggaaca gtccctatcg cgaccgtact gttgaggaaa atctcgctct
     6001 cttccgccgc atgcgggcag gcgaattccc caacggggca cgcgtgctgc gcgcgaaaat
     6061 cgacatggca tcgggcaaca tcaatctgcg cgatccggtg ctctaccgca tcctgaacgc
     6121 aatccatccg cgcaccggcg atgcttggaa aatctatccc agttatgatt tcgcgcatgg
     6181 gcagtccgat gcgatcgaac acatcaccca ttcgctctgc acgctcgaat tcgaggatca
     6241 ccggccgctc tacgattggt tcctggacaa tcttccggtg ccttcgcgtc cgcaccaata
     6301 tgagttcgcg cgtttgaaca tcgccaatac cttgctttcc aaacgcacgc tgaccgaact
     6361 cgtgcggggg gggcatgtct cgggatggga cgatccgcgc atgccgacgc tggcgggact
     6421 gcgccgccgc ggcgtgccgc ccgaagccgt gcgtgatttc gtgcgccgcg ccggggtggc
     6481 gaaggccaat agtgtcgtgg atgcggcggc gttcgactat gcggtgcgcg aagtcctcaa
     6541 cagacgcgcg ccacgccgca tggcggtgct caaccccctt aagattgtga tcgagaatta
     6601 tccggaaggg cagactgaag agcttgaggc gatcaacaat cccgaagacc cttccgccgg
     6661 ctcgcggcgc attccgtttg gccgggagct ttatgtcgag cgcgaggatt ttatggagga
     6721 cccgccgaag aaattctttc gtctgtcgcc gggccgcgaa gtgcggctgc gttatgccta
     6781 tttcatcacc tgccgcgagg tggtgaagaa tgcagccggc gaggtggcgg aattgcgctg
     6841 cacctacgac ccggcgacca aaggcggcaa tgcgccggat gatcgaaagg tgaaggccac
     6901 gctgcattgg gtcgcggccg ccagcgccct accggcggaa atccgcatct tcaatccgct
     6961 gttcacgcgt cccgatcccg gcgctgacgg cgatttcgcc caggacctga atcccaactc
     7021 gctggaaata ttgcccggcg cgcagctcga gcctgcactc ggcgatgcgc gcgaaggcga
     7081 agcgatccag ttcgaacgcc agggttattt ctgcctcgat gcggggccga gccgcaatcg
     7141 cccggtgttc aaccgcacgg tcagtttgcg cgattcgaaa tgaccgcgag gacagcatca
     7201 tgaacggcgc ggaaagcctg gtgcgcacgc tgctcgcctc tggcatcgac acctgcttcg
     7261 ccaatcccgg caccagcgag atgcattttg tcgccgcgct cgaccgcgcc cctggcatgc
     7321 gctgtgtgct cggcctgttc gaaggcgtgg tgacgggtgc agccgatggt tatggccgca
     7381 tggccggcaa gcccgccgcg accctgatgc attgcgggcc tggcctcgcc aatgggttgg
     7441 cctatctgca caacgccaag cgcgctagca caccgatcgt gaatattgtt ggcgatcagg
     7501 cgacctatca tgcgccgctg aatccgccgc tcgcctccga tgtggaaggg atcgcgcggc
     7561 ccgtgtcgca atgggtccgc aaggtgacgc aaaccgcaac gctcggcgcc gacgcggccg
     7621 aagccgtgcg gagcgcgtat caaccgccgg gcggggtggc cacgttgatc ctcccatcag
     7681 acgtgagctg gaacgacggc ggcgcaccgg gcacgccgct tcctattccc gcgcctgcga
     7741 acgtggcgaa cgacgcggtt attgaggccg caagcgcgct gcgccgtggc gaacgcacgg
     7801 tgattcttct cgctggcgcg gcgctgcacg ccgctgcgct ggcgatcgcc cacaatatcg
     7861 ccgaggcgac gggtgcgcgg atcatggcgc ccaccttcaa cgcgcggatc gcgcgtgggg
     7921 cagggcgtcc attgctcgac atcgtgccct atccggttga tgaagccgtc gcccaactga
     7981 aagatgtgaa gcacctcatt ctggtaggca cgagcgagcc cgtcgccttt ttcgcctatc
     8041 ccggaaagcc cggcatcatg tcgccgaaag atgcggtgtc gcatgtgctg gcgcgggccg
     8101 acgaggattt gcgcgatgcg ctgacgcgtc tcgcgtcgga actgaattgc ccaaacacgc
     8161 cgatcccgga atatcgcgcc aatgcgttgc cgggcgcagg acccatgagc gcggaaggcg
     8221 cgggtctaac gcttgccgca ctcctgcccg agcaggcgat cctgatcgac gaggcgatca
     8281 ccttgcgcgg gccgttcttc gcggcgacca aaaccgctgc ccctcatgat tggctgcagg
     8341 tgccgggcgg cgccatcggc ggagggttgc cgctcgcgac cggtgccgcc attggcgcgc
     8401 cgggccggcg ggtgatcgcg attcaggccg atggttcggc catgtatacg atccaatcat
     8461 tgtggacgca ggcgcgcgag aatctcgatg tcacgaccct gctgctgtcc aatcgcaaat
     8521 acgccatcct gttccacgag ctgaaacagg tgggcgccac cgaaggccgc gcctcgagcg
     8581 atctcttcgc gctcgaccgg cccgaactcg attggatcaa gctctccgaa agcatgggcg
     8641 tggaagcggc gcgcgccgag acgctcgaac gctttgccgc gcttctgacc catgccaatg
     8701 gccgacgggg tccgttcctc atcgaactcg tgctgtgatc cgccgaaaaa tgctaggatg
     8761 ccgcttcaca tcggatggcg agacccatga caatttcgac gttgaaacgg atcggcatcg
     8821 gtggtgcggt gctggcgctc ggacttggcg gcattgatgc attcgcagcg gcgccaaagc
     8881 tcgatggcac ggtcaagcaa gagagcgtgg cggtgaaagg cgcgcaacgg accttcgtcc
     8941 tttatgtgcc gcatgatctg aaaccgaatg cgccgcttct gttcgttttc cacggctcgg
     9001 gcggcgacgg cgcttcgatg cgcgatgtga cggcgttcga attcgacatg ctggcggacc
     9061 gcgacggctt ccttgtcgcc tatccggatg gttatcagac aacctggaac gattgccgca
     9121 agggatcgcc gcagcccgcg cgccggatga acatcgatga cgaaagcttc gtcgatgcga
     9181 tgatcgcaaa ggaagcagcc gagcacgcta tcgacaggaa gcgtgtgttc tcggcgggct
     9241 ggtcgaatgg cggacaactc ggctaccgct tcgcgatgga gcgggcgaat gttttcaccg
     9301 gcgttgccgc gctttcggcg accgtgccgg tgcccgcaaa tcttgcctgt acgccatcgg
     9361 gcgccgccat gccgatgatg atcatcaacg gcacggccga tccgataaat ccgttcggcg
     9421 gcggcaatgt gatgctgggg ggatcaaatc tcggcgccgt ctattcgtcg caagacggcg
     9481 cgcaatattg ggcgaagctg aacggcatca cctctggacc tcaaaccaaa cgcctgccgc
     9541 acagaaatgc ctcagacccg acctatgtcg atgaaatgac atggtcatcg gccggcaagc
     9601 cactcgtcgt gatgtatgcg gttcacggcg gcggacacgt tgtgccgacg cgtgtccccc
     9661 aggattgggg aggtcttggc cgcgagaccg gcgatctcga tgctcccgtt gcgatctggg
     9721 atttcttctc gaaattgccc agccgcccat aaaaccttag tcgcccataa gaaaaaaaga
     9781 gcccgcatcc gaatggatcg gactcttcat tgtcaatcct tggcg
//
LOCUS       DASYEG010000130        13201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4244156, whole genome shotgun sequence.
ACCESSION   DASYEG010000130 DASYEG010000000
VERSION     DASYEG010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 13201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13201
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4244156"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VGM72_05505"
     CDS             <1..291
                     /locus_tag="VGM72_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEY4264753.1"
                     /translation="YKIEAPSVVGHPAFERAHRTHINTVENLVLMIPLLWIASVFYGG
                     QIPFWLGLVWVVSRILYAIGYAQTNAQLREPGAGLGFLSLIVLFVLAVIGLT"
     gene            complement(320..730)
                     /locus_tag="VGM72_05510"
     CDS             complement(320..730)
                     /locus_tag="VGM72_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEY4264754.1"
                     /translation="MITEMWALFASVILGFVHIFAQALSMNAQRGVDYNVGPRDEAMP
                     PLTGVAGRLSRALRNFLETFVLFVAAVVLAQLVNVHNGLILRGAWLYVVGRVVYLPLY
                     AFGIPYIRSLAWGAASAGIGLILAGVFLGTMARA"
     gene            complement(727..1578)
                     /gene="kdsA"
                     /locus_tag="VGM72_05515"
     CDS             complement(727..1578)
                     /gene="kdsA"
                     /locus_tag="VGM72_05515"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEY4264755.1"
                     /translation="MTTAPNAIVRIGSVEVGNRRPLSIIAGPCALESRDHAFEMASAL
                     KEIAAKAGVGLIYKTSFDKANRTSANSARGLGLELALPILADIRERFGMPVLTDVHER
                     EQCAVAAEAVDVLQIPAFLCRQTDLLVAAAETGRVVNVKKGQFLAPWDMKNVIAKITD
                     AGNPNVLATERGVSFGYNTLVSDMRALPILASFGAPVVFDATHSVQQPGGQGTRSGGE
                     RDMVPVLARAAVAIGVAAVFMETHQDPDSAPSDGPNMLKLKDVPDLLSELVELDRIAK
                     KRQAAQP"
     gene            complement(1596..2285)
                     /locus_tag="VGM72_05520"
     CDS             complement(1596..2285)
                     /locus_tag="VGM72_05520"
                     /inference="COORDINATES: protein motif:HMM:NF006093.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264756.1"
                     /translation="MAADEPTYFNDLRQISTDSIAGVLLKRGLRNQWVRGPMPLRMDF
                     PRTVGRAFTMRFIPVREDIEGAFMKKPPVNRDAVEMMPSGCVVIADARGTRDAATFGD
                     IVVERMVKRGVAGIVTDGAVRDRMGLLASKMPIWMSGITAPPPAASLMLVEWQHPIGC
                     GGVAVFPDDIIVADADGVVVIPQGLLEEVVDRGMDQEHLDKWQMDQIKRGVALCDLAP
                     MPRNDGRSSNN"
     gene            complement(2827..4455)
                     /locus_tag="VGM72_05525"
     CDS             complement(2827..4455)
                     /locus_tag="VGM72_05525"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004815797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY4264757.1"
                     /translation="MARLIFITGGVVSSLGKGLASAALGALLQARGYTVRLRKLDPYL
                     NVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFTGVASSKSDNVTAGRIYQGIIAKE
                     RQGLYLGRTVQVIPHVTDAIKEFVMAGHDGVDFLLCEIGGTVGDIEGLPFFEAIRQLG
                     NELGPERSAFVHLTLVPYITSAGELKTKPTQHSVKELRSIGIQPDILLCRSDMEIPLE
                     DRRKIALFCNVRPERVIQAMNLETIYDAPIAYHEQGFDAQVLDVFGIKDAPPPDLSRW
                     RAVVDRVLKPEGEVNIAVVGKYMQVKDSYKSLIEALIHGGLANNVRVNVEWLESEVFE
                     KQDAAVYLEDRNGILVPGGFGERGTEGKIEAAKFARERKVPFFGICFGMQMAVIEAAR
                     NLAGLESAGSTEFGQPRHPVIALMTEWTRGNEIERRNADTDLGGTMRVGAYEAVLKPG
                     SRVADIYGATNISERHRHRYEVNIGYRRQLEEHGLLFSGLSPDGILPEIVEIPDHPWF
                     IGVQFHPELKSKPLDPHPLFASFISAAVQQSRLV"
     gene            complement(4553..4954)
                     /gene="secG"
                     /locus_tag="VGM72_05530"
     CDS             complement(4553..4954)
                     /gene="secG"
                     /locus_tag="VGM72_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEY4264758.1"
                     /translation="MQAVLIIVHLLVAMALIFVVLLQRSEGGALGMGGGGSGLGGLFS
                     PRGAASTLTRTTAILGLVFFITSMSLTLLSLGARRPTSILNQVAPGQTAPANPNAPPT
                     LPSRAPALPSANPAAPPPAPAPALPAVPPTR"
     gene            complement(5159..6136)
                     /locus_tag="VGM72_05535"
     CDS             complement(5159..6136)
                     /locus_tag="VGM72_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264759.1"
                     /translation="MIAKRLYDTARVLVYDPVASNRNATRASLHSLGYRNVEGVTALD
                     VLANRLMTDPPDLLLAEVAGAESETCGMIQAIRQGQMGDNPFIMVIATTWRRDGSIIG
                     QVLNSGADDLVARPVSTTTLGERVRLLTERRKGFVVTSQYIGPDRRRDVRSDEGGAEC
                     VEAPNPLRFRMLENLSEEEVARRMLESVLQGKEKLNLGKVRRDAVQLCLQWRMLEKRD
                     PAARDFCEILSRMGQLAVEMKRRAKLAKHEAGEEWCDSIAGSVRALTEMVARDGHSVV
                     IADYQPALNLLNHATSALGRIFARDEVEPERLVELDRIVAVRNARSAAA"
     gene            complement(6245..6994)
                     /gene="tpiA"
                     /locus_tag="VGM72_05540"
     CDS             complement(6245..6994)
                     /gene="tpiA"
                     /locus_tag="VGM72_05540"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY4264760.1"
                     /translation="MRMLLAGNWKMNGLRNALGEIRALKRGLADEPARGDVLVCPPAT
                     LIADAVKAAEGGSVAIGGQDCHALASGAFTGDISAEMLRDAGATAVIVGHSERRQYHG
                     ETNVIVAGKVRAARRAGLAAILCVGESGPERDAGSAVAVVTEQLQASIPAELNGKDLV
                     IAYEPIWAIGTGKTPQSSDVGQMHEAIRAVLKTRFGGEGGEMRILYGGSVKPENAADI
                     LALANVDGALIGGASLKAADFLAIIRAAPRR"
     gene            7120..9012
                     /locus_tag="VGM72_05545"
     CDS             7120..9012
                     /locus_tag="VGM72_05545"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEY4264761.1"
                     /translation="MLQQLRIASKSWVASVVIGILVLAFALWGVADIFTASGVDNVVA
                     KVGGTEISANDYDLQLKNQIRNLSAQTKNQLTMEQAKAIGLDKNVLDQVIARAALDEQ
                     VKSLGLTASQDTIATQIRTNQSFRGADGSFDPNLFLRTLQDNGMSEQAFVAATSSDIA
                     REQLLNAVTDGMVAPPGLARLLYDFINEQRVIDYLVVTPEEAGQVPEPSQADLEAYHK
                     AHAAQFSSPEYRSFDYVQIGPDQVAGEIQISDDDIKAEYDSHKADYDKPEQREVEQIA
                     FPTQEAANAAAMKIKTGADFTALAHERGLKDADLKLGTLAAAGLDPKLSAPVFAVPEG
                     GVTPAVQGPFGWVILRAAKVIPGEMKTLDQVKDEIKAGLVKTRDAAKLTEIANKFEDE
                     RGSGGTLAEAAMKEGLMLHHVAAIDRMGMTPEKSEPEIAKIPQVLEQVFQTEVGEDSD
                     LFQSPDGQYFAVKVNSSMPPAIKPLDSVREEVKEGFIADARNKLLQTKVQTLAAEAMK
                     SGSLAAAGKALGHPPVTSMPLKRGDMGDVFSMQLIGQLFAVPPGAVITGPAGKGNGIV
                     LARVVKVTHPEPDVSSADYVNFRRTAAQQLSMTAVDSLAAAARKKAGVNIHQATVQRV
                     LGDAQQ"
     gene            9024..10544
                     /gene="trpE"
                     /locus_tag="VGM72_05550"
     CDS             9024..10544
                     /gene="trpE"
                     /locus_tag="VGM72_05550"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEY4264762.1"
                     /translation="MDIAPGFAAFASAYEKGQPQALFVRRIADLETPVSAFLKLAESR
                     TNAFLFESVQGGETRGRYSIIGLKPDLIWRCRAGAAEINRDACVDADRFVPDADADAP
                     LRSLKRLVKEAEMTLPAELPPMSVGLFGYLGYDIVRHMEKLPSAPPDVLGLPEACLLR
                     PTITAIFDNVRDEIIVVTPVWPQAGISARAAYARGTERLEEIVSDLERMGTPQPTSSV
                     EPTLGPAESNTSHADYLAIVEKAKAYIRAGDIFQVVPSQRFRRSFSLPPFALYRALRR
                     LNPSPFLYFLNFPGFAIVGSSPEILVRLRDSKVTIRPIAGTRPRGKTPDDDSRLAEEL
                     MADPKERAEHLMLLDLGRNDVGRVVQTGSVTVTDQFFIERYSHVMHLVSNVEGHIRDG
                     LDAVDALVAGFPAGTLSGAPKVRAMEIIDELEKEKRGVYAGAVGYFAANGTMDTCIVL
                     RTAVVKDGVMYVQAGGGVVADSDPEAEFQETVNKAKALMAAADEAVRYASAPKRGP"
     gene            complement(10605..10868)
                     /locus_tag="VGM72_05555"
     CDS             complement(10605..10868)
                     /locus_tag="VGM72_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264763.1"
                     /translation="MTKHVHMSSHSRPPRAQGAEHSSSLSWFVLAALVFYAAMIWFGG
                     EHPAWSPIAQFATNMMDRWDESVAPAVLAPLREKGLSVLHHGS"
     gene            complement(11084..12934)
                     /locus_tag="VGM72_05560"
     CDS             complement(11084..12934)
                     /locus_tag="VGM72_05560"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4264764.1"
                     /translation="MKKLLCGISVAACLGLAIGVGPVKVAAQNAGGPAIVIQGGTLID
                     GNGGAPVANSVVVIQGNRILAVGRAGAVPVPAGAQTINASGKYVLPGLWDAQTNIAWY
                     WGEVFLNQGVTSTVDIGDGEEVSIAQRDAVNHGKAIGPRQMVGIGHLGGIRGGAMPTG
                     LESALATRKDPKSPDDARAFARRFLAAGADMVMLHDGSYAPEIYQTAFDEAHKAGKPA
                     FCRCGGPKTGPKEGALAGADVFPHSTGIGAAVAKDGTRANNELDRYAEMDDAKANDLI
                     KVLVEHHVNLVPTLVHDSPGYPKGWARFQAEARKTFQDPNLRAYYDDGNIRSFFATYN
                     RSDAGAVRDRRTRGFANMLRFNRMYDAAGGHVLAGGDTNNQKAPGFILHDEMETMAEA
                     GIPAMHVIQGATKWTAEALHVADKIGTVETGKIADVIVVDADPLADVANLRKVSTVVF
                     DGKPVELGYHAWYGTPFGGGAEDRAVVDNLEWTQSLKQATFRALQGEAAMREAPDPLE
                     NPQPAIEAITPVMIAEGSPTTTLTLKGFNFVRRSRVYFDGRAVPYKRINATELQVSLD
                     DNLLKRAGRFDIEVKNPEPLANKQWGDGQSNKAHLLVMYGFTAIRTANAN"
BASE COUNT         2502 a         4180 c         4018 g         2501 t
ORIGIN      
        1 tacaaaatcg aagcgccttc ggttgtcggc caccccgcct tcgagcgcgc ccatcgcacg
       61 catatcaaca cggtcgagaa tctcgtgctg atgatcccgc tgctgtggat cgcgagtgtg
      121 ttctatggcg gccagattcc attttggctc ggcctcgtgt gggtggtgag ccgcatcctc
      181 tacgcgatcg gctatgcgca gacgaatgcg caactccgcg agccgggcgc gggattgggc
      241 tttctgtcgc tgatcgtctt gtttgtgctg gcggtgatcg gcctgaccta aagggcaatc
      301 cggccaagcg ccctgtgtct catgctctcg ccatggtgcc gaggaaaacg ccggcgagta
      361 tcagaccgat accggcgctg gcggcgcccc acgccagcga gcgaatatag ggaatgccga
      421 acgcgtaaag cggcagatag accacgcgcc ccacaacata gagccacgca ccccgcagga
      481 tgaggccgtt gtgcacattg acgagctgcg ccagcacgac cgccgcgacg aacaatacga
      541 atgtttcgag aaaattgcgc aaggcgcgcg aaagccgccc ggcgacgccc gtcagaggcg
      601 gcatcgcttc atcgcgcgga ccgacattgt aatcgacgcc gcgctgcgca ttcatgctta
      661 gcgcctgtgc aaagatatgg acaaagccaa ggatgacgct cgcgaagagc gcccacatct
      721 cggtaatcat ggctgcgccg cctgacgttt cttggcgata cggtcgagtt cgacgagttc
      781 cgaaagcagg tccggtacat ccttcagttt cagcatattg gggccatcgg acggcgcgct
      841 gtccggatct tgatgcgtct ccatgaagac ggcggcaacg ccgatggcga cggctgcgcg
      901 cgccaacacc ggcaccatgt cgcgctcacc acccgaacgc gtgccctgtc cgcccggctg
      961 ctgcacggaa tgcgtggcat cgaacaccac cggcgcaccg aaggatgcga ggatcggaag
     1021 cgcgcgcata tcggaaacca gcgtgttgta gccaaaggag acgccgcgtt ccgtggcgag
     1081 cacattggga ttgccggcat ccgtaatctt ggcaatcacg ttcttcatgt cccaaggggc
     1141 gaggaactga cctttcttga cattgaccac acgtcccgtt tccgcggcgg ccaccagcag
     1201 atccgtctgc cggcatagga aggcggggat ctgcagcaca tcgaccgcct ccgcggcgac
     1261 ggcgcattgt tcacgctcat gcacgtcggt cagcaccggc atgccaaagc gttcgcggat
     1321 atcggcgagg atcggcaggg cgagttccaa cccaagcccg cgggcggaat tggcgctggt
     1381 gcgattggct ttgtcgaagc tcgtcttata gatcagccct accccggcct tggccgcgat
     1441 ctccttcagc gccgacgcca tctcgaaggc gtgatcgcgc gattcgagcg cgcatggccc
     1501 cgcgatgatc gaaagcggtc ttcgatttcc gacttcgacg gaacctatgc ggacaatcgc
     1561 gttgggtgcg gtggtcatct ttttcctggg ccgcgtcagt tgttcgacga tcttccgtcg
     1621 ttgcgaggca tgggcgccag atcgcaaagc gcgacgccgc gcttgatctg gtccatctgc
     1681 catttgtcga ggtgctcctg atccatcccc ctgtccacaa cctcttccaa caggccctgc
     1741 gggatcacca ccacgccatc ggcatcggcg acgatgatat catcggggaa cacggcgaca
     1801 ccgccacaac cgatcggatg ctgccattcc acgagcatca ggctggccgc cggcggtggc
     1861 gcagtaattc cgctcatcca gatgggcatc ttgctcgcga gcaaacccat ccggtcgcgc
     1921 accgcgccgt ccgtgacgat gcccgcaacg ccgcgcttca ccatgcgctc caccacgatg
     1981 tcgccgaacg tggccgcgtc ccgcgtgccg cgcgcatcgg cgatgaccac acagccggac
     2041 ggcatcatct cgaccgcgtc ccggttgacc ggcggtttct tcatgaacgc tccctcgata
     2101 tcctcgcgca ccggaatgaa gcgcatggtg aaggcacgtc cgacggtgcg gggaaaatcc
     2161 atcctgagcg gcatggggcc tcgcacccat tgattgcgca agcccctctt caacaagacg
     2221 cccgcgatcg aatcggtcga tatctgccgc aaatcattga agtacgtcgg ctcgtcggct
     2281 gccatggcac catcctgatc gttcacggtg tcccggactt agcggaccgg caccacgccg
     2341 tccagccgga cagcgacccg gtcccaagcc tttcctgggc caagcctttc ctggggagaa
     2401 ctggagggta tgaacacccc catggcggca acgatcattt acgacggcgg ccgcccatcg
     2461 gattttccag agtccggaaa aattcgcgcg gcgtctggct tcagcgcggc tccgacgttc
     2521 gcggcggggt ctgcgtgcat tcttccgcct ctaatttcgc cgcctccgtg tcaagatcca
     2581 cggcatagct gttcaggttc gccgcggcca tcgggtcaca tatccgcgcc gcatgatcgc
     2641 gggcccgcat ggcttttgcc cgcaactcgc gaatccgcgt tgcgcattca gggagaaaat
     2701 tatcgagttc ggacatagtc ttaaccgaag ttgcccccgg ttaatgcagt cggcaatatt
     2761 tggttcccga catttcggcc gggcgcgctc acattttacg tcttgcggac ggaaaagccc
     2821 ttggagttag accaggcggc tttgttgtac ggcggcgctg ataaacgagg cgaacagcgg
     2881 gtgcggatcg aggggcttgg acttcagctc gggatggaat tgcacgccga tgaaccaggg
     2941 atggtccggt atctcgacga tctcgggaag aatcccgtcc ggcgaaagtc cagagaacaa
     3001 cagcccgtgc tcctccaatt ggcggcggta gccgatgttc acctcatagc ggtggcggtg
     3061 gcgctcgctg atattggtcg cgccgtagat gtctgcgacg cggctgccag gtttcaggac
     3121 cgcctcatag gcgccaaccc gcatcgtgcc gccgagatcg gtgtcggcat tgcgccgttc
     3181 gatctcattg ccgcgcgtcc attcggtcat gagcgcgatc accggatgac gcggctgacc
     3241 gaattcggtt gagcccgcgc tctccaaccc ggcgagattg cgcgccgctt cgatcaccgc
     3301 catctgcatc ccgaagcaga ttccgaaaaa cggcaccttg cgttcgcgcg cgaatttcgc
     3361 cgcctcgatc ttgccttctg tgccgcgctc accgaagccg ccgggcacca ggatgccgtt
     3421 acggtcttcg agatagaccg cggcatcctg cttctcgaaa acctccgact cgagccattc
     3481 gacattgacc cgcacattgt tcgcgagccc gccatggatg agggcttcga tcagcgattt
     3541 gtacgaatcc ttgacctgca tatatttgcc gacaacggcg atattgacct cgccctcggg
     3601 tttcagcacg cggtcgacga cggcacgcca gcgcgaaaga tcgggcggcg gcgcatcctt
     3661 gatcccgaag acgtcgagca cctgcgcatc gaacccctgc tcgtgatagg cgatgggggc
     3721 gtcgtagatg gtctccagat tcatcgcctg gatgacgcgc tcggggcgta cattgcagaa
     3781 cagcgcgatc ttgcggcggt cttccagcgg aatctccatg tcggagcggc agagcagaat
     3841 gtcgggctgg atgccgatac tgcgcagctc cttgaccgag tgctgggtgg gcttggtctt
     3901 caactctccg gccgaggtga tgtagggcac cagcgtcaag tgcacgaaag cagagcgctc
     3961 gggcccgagt tcgttgccga gttgccggat ggcttcgaaa aaaggcaacc cttcgatgtc
     4021 gcccaccgtg ccgccgatct cgcaaagcag gaaatcgacg ccgtcatgtc cggccatgac
     4081 gaattccttg atggcgtcgg tgacgtgcgg gatgacctgc accgtgcggc cgagataaag
     4141 gccctgacgc tccttggcga tgatgccctg atagatgcgc cccgcggtca cattgtcgga
     4201 tttcgacgac gcgacgccgg tgaagcgttc ataatggcca agatcgagat cggtctccgc
     4261 gccatcgtcg gtgacgtaga cctccccgtg ctgatagggg ctcatcgttc ccggatcgac
     4321 attgagatag ggatcgagct tgcgcaggcg cacggtatag ccacgcgcct gcagaagcgc
     4381 gccaagagcc gcggaagcca agccttttcc cagggaagag accacgccgc cggttatgaa
     4441 gatcaaccgc gccatggaat tggaccatgt ctcaaggttg gctcgcgctc aagcgttttc
     4501 gccatgcaac gtgcggattc atgtgcagaa gagtgcctga tgttttcgcg actcaccgcg
     4561 tcggcgggac ggcaggcagg gccggcgccg gcgccggcgg tggtgcggcc ggattggcgc
     4621 tcggtagagc cggtgcgcgc gagggcaaag tcgggggtgc gttcggattg gcgggggccg
     4681 tctgccccgg cgccacctgg ttcaggatcg aggttggacg ccgggcgccg agtgacagca
     4741 atgtgagcga catgctggtg atgaaaaaga ccagccccag gatcgccgtg gtccgggtga
     4801 gcgtgcttgc cgcgccgcgc ggcgaaaaca gaccgccaag tcccgagccg ccgccgccca
     4861 tgccgagcgc gccgccttcg gagcgctgca acagcacgac gaagatcagc gccatggcga
     4921 ccagaagatg aacgataatg agtaccgctt gcataggctg ccgggggtcc gttccgtgga
     4981 gggaggggat atcgatgcgt cgaggccccg cgtcaataga cgaaatcggc aacccggaaa
     5041 acagaaatcg gaaaatcacc ggcggaaagt ccaaaaactc cgctgaatcg gcgcgtttaa
     5101 cccgcatcgc gcggcatttg cgcccggctt cgcgagagcc ttcgcaagca gggtccgatt
     5161 acgccgcggc ggagcgtgcg ttgcggaccg cgacgatccg gtcgagttcg acaaggcgct
     5221 cgggttcgac ctcgtcgcgg gcgaagatcc ggcctagcgc cgaggtggca tggttgagaa
     5281 gattgagggc cggctgataa tcggcgatga caacgctgtg cccgtcgcgc gccaccattt
     5341 cggtgagagc tcgcaccgac ccggcgatcg aatcgcacca ctcctctccc gcttcatgtt
     5401 tggcgagttt ggcgcggcgc ttcatctcga ccgccagttg ccccatgcgt gacaggattt
     5461 cgcagaaatc gcgggcggcc ggatcgcgtt tctccagcat ccgccattgc aggcagagtt
     5521 gcaccgcgtc ccgccgcact ttcccaagat tgagcttttc cttgccctgc agcacgctct
     5581 ccagcatccg acgcgccact tcttcctccg aaagattctc cagcatccgg aatctcagag
     5641 gattgggggc ttctacgcac tccgcgccac cttcgtcgct gcggacatcc cgccgccggt
     5701 cggggccgat atactgcgaa gtgaccacga aacccttgcg gcgctcggtg agcaggcgaa
     5761 cgcgctcgcc tagggtggtg gtcgagacgg gacgcgccac caggtcgtcg gcgcccgaat
     5821 tcagcacctg cccgatgatc gagccgtcgc gccgccaggt cgtggcgatc accatgatga
     5881 acggattgtc gcccatctgg ccctgacgga tggcctggat catgccgcag gtttcacttt
     5941 cagctcccgc cacctccgcc agcagcaggt ccggcggatc cgtcataagg cggttggcca
     6001 ggacgtcgag cgccgtcacg ccttcgacgt ttcggtagcc gagcgagtgc agtgaggctc
     6061 gtgtggcgtt gcggttcgag gcgacgggat cgtaaaccag tacgcgcgcg gtatcgtaaa
     6121 gccgttttgc aatcatgtgt tttccccggc ggatagccga gagctaacat gccgcccgtt
     6181 aagaggtgtt gcgcgaattg gaacgcaatt gtgcggcaat cgtcgaaaat tctaatcgat
     6241 ggcctcagcg ccggggcgcg gcccggataa tagccagaaa atccgccgcc ttcaggctcg
     6301 cgccgccgat gagggcgccg tcaacattgg cgagggcgag gatgtccgcg gcattctcag
     6361 gcttgaccga gccgccataa aggatgcgca tctccccccc ctcgccgcca aagcgcgtct
     6421 tgagaacggc ccggattgct tcgtgcattt ggccgacatc ggaggattgc ggcgtcttgc
     6481 cggtgccgat cgcccagatc ggctcatagg cgatgacgag atccttgccg ttcagctcgg
     6541 cggggatgct tgcctgtagc tgttccgtca caaccgcaac ggcgctgccg gcgtcgcgct
     6601 ccgggccgga ctcgccgacg cagagaatcg ccgccagacc cgcgcgtctc gctgccctga
     6661 ccttgccggc cacgatgacg ttggtttctc catgatattg gcggcgttcg gaatggccca
     6721 cgatcaccgc ggtcgccccg gcatcgcgca gcatttcggc cgagatatcg ccggtaaagg
     6781 ctccggaagc caacgcatgg cagtcctggc cgccgatggc gacggacccg ccttcggcgg
     6841 ctttcaccgc atcggcgatc agcgttgccg gggggcagac gagcacgtcg ccgcgcgcgg
     6901 gttcatcggc aagtccccgc ttcagcgccc ggatttcccc cagagcgttc ctcagcccat
     6961 tcatcttcca attgccggcc aacaacatgc gcatgcttga tcctctgccc aatgccgtgc
     7021 ttgatccggc gggcggcaac cccatttttc gttaaagcct tgccgcgttc cgggcctctc
     7081 cctatcatcc gcggcctttt gagactattt ggttttccca tgctgcaaca acttcgcatc
     7141 gcctccaaat cctgggttgc atccgtcgtc atcggcattc tcgtgctggc gtttgccctg
     7201 tggggcgtgg ccgatatctt taccgcgtcc ggcgtggaca atgttgtcgc caaggtgggc
     7261 ggcacggaga tttcggcgaa cgattacgat cttcagctca agaaccaaat ccgcaatctg
     7321 tcggcccaga ccaagaacca gctcaccatg gagcaggcca aagcgatcgg cctcgacaag
     7381 aatgtgctcg atcaggtgat cgcgcgcgcg gccctcgacg agcaggtcaa atcgctcggc
     7441 ctgaccgcct cacaggatac catcgccacc cagatacgaa cgaatcagag tttccgaggc
     7501 gcggacggca gcttcgatcc caatcttttc ctgcgcacgc ttcaggacaa cgggatgtcg
     7561 gagcaggcct ttgtcgccgc tacgagttcg gacatcgcgc gtgagcaatt gctcaacgcg
     7621 gtgaccgacg ggatggtggc gccgccaggt ctggcgcgcc tgctttatga tttcatcaac
     7681 gagcagcgcg tcatcgacta tctggtggtg accccggaag aagccggtca ggtccccgaa
     7741 ccgagccagg ccgatctcga ggcctatcat aaggctcacg ccgcccagtt cagttcgccg
     7801 gaatatcgtt ctttcgatta tgtgcagatc ggccccgatc aggtcgccgg cgaaatccag
     7861 atttcggacg acgatatcaa ggccgaatat gattcgcata aggccgatta cgacaagccc
     7921 gaacagcgcg aggtcgagca gatcgccttt ccgacccagg aagctgccaa cgccgctgcg
     7981 atgaagatca agacgggagc cgatttcacg gcactcgccc atgagcgcgg attgaaggat
     8041 gcggatttga agctcggcac cttggcggcg gccggcctcg acccaaagct ttctgcgcct
     8101 gttttcgcag tgcccgaagg cggtgtgaca cctgccgtgc aggggccgtt cggctgggtg
     8161 atcctgcgag ccgccaaggt aatccccggc gaaatgaaga cgctcgatca ggtcaaggat
     8221 gagatcaaag ccggcctggt gaagactcgc gatgccgcca aactcaccga gatcgcaaac
     8281 aagtttgaag atgagcgcgg aagcggcggg acacttgcgg aagcggccat gaaagaaggt
     8341 ttgatgctcc atcatgtggc cgctatcgac cgcatgggga tgacccctga aaagagcgag
     8401 ccggaaatcg caaagatccc gcaagtgctg gaacaggtat tccagacaga ggtcggggag
     8461 gacagcgacc tgttccagag tccggacggc caatatttcg cggtcaaggt caacagctcc
     8521 atgccgcccg cgatcaagcc tctggattcg gtgcgcgagg aggtcaagga aggcttcatc
     8581 gccgatgcgc gaaacaaatt gctgcaaacc aaggttcaga cgctcgccgc ggaagcgatg
     8641 aaatcaggca gccttgcggc ggcaggcaaa gcgcttggcc atccgccggt caccagcatg
     8701 cccttaaagc gcggtgacat gggcgatgtc ttttcgatgc agctgatcgg tcagcttttc
     8761 gcggttccgc cgggcgccgt gatcaccggc ccggccggca agggcaacgg catcgttctc
     8821 gcgcgcgtgg tgaaggttac ccatcccgag cccgacgttt cgagcgcgga ttatgtcaat
     8881 ttccgcagga ccgccgcaca gcaattgagc atgacggcgg tggattctct ggcggccgcc
     8941 gcgcgcaaga aagcgggcgt gaacatccat caagcgaccg tccagcgcgt cctcggcgac
     9001 gcgcagcaat agatttcaac gtcatggata tcgcgcccgg cttcgccgct tttgcgtccg
     9061 cctacgaaaa gggccagccg caggctctct tcgtgcgcag gatcgccgat ctcgaaactc
     9121 cggtttccgc atttctgaaa ctcgccgaaa gccgcaccaa tgccttcctg ttcgaaagcg
     9181 tgcaaggcgg cgagacccgc gggcgctatt ccatcatcgg tctgaagccc gatctgatct
     9241 ggcgctgccg ggccggcgcg gcggagatta accgcgacgc ctgcgtggac gcggatcgtt
     9301 tcgtccccga cgcggatgcg gatgcccccc ttcgttcgct caagcgcctc gtcaaggaag
     9361 ccgagatgac attgccggcg gaattgccgc cgatgtcggt cggcctgttc ggctatctcg
     9421 gctacgacat cgtgcgtcac atggagaagc tgccctctgc gcctcccgac gtgctcggac
     9481 ttcctgaagc gtgtctgttg cgcccaacca ttaccgccat cttcgacaat gtccgcgacg
     9541 agatcatcgt ggtgacgccg gtatggccgc aagccggaat ctcggcgcgc gcggcctatg
     9601 cgcgtggcac cgagcggctc gaggaaatcg tgagcgacct cgaacggatg ggcacgccgc
     9661 agccaacctc cagcgtcgag ccgacgctgg ggccggcgga atccaatacc agccacgcgg
     9721 attatcttgc catcgtcgag aaggcaaagg cgtatatccg cgccggcgac atcttccagg
     9781 tggttccaag ccagcgattc cggcggagtt tttcattgcc gccctttgcg ctgtatcgcg
     9841 cccttagacg gctgaaccca tcaccctttc tctattttct gaattttccc ggctttgcaa
     9901 tcgtcggctc aagtccggag atcctggtgc gtctgcgcga ttccaaggtc accattcgcc
     9961 cgatcgcggg cacgcgtcca cgcggaaaaa ctccggatga cgattccagg ctcgccgagg
    10021 aattgatggc cgatccgaag gagcgcgccg agcatctcat gttgctcgat ctcggacgca
    10081 acgatgtcgg ccgtgtcgtg caaaccggca gcgtcaccgt gacggatcag ttcttcatcg
    10141 agcgttattc gcatgtgatg catctggtgt cgaatgtcga aggacatatc cgcgacggtc
    10201 tcgatgcggt cgatgccctg gttgcgggct ttccggccgg cacgctgtcg ggtgcgccca
    10261 aggtgcgcgc gatggagatc atcgacgaat tggaaaagga gaagcgcggt gtctacgccg
    10321 gcgcggtggg ctatttcgcc gcgaatggca cgatggatac gtgcatcgtg ctgcgcaccg
    10381 ctgtcgtcaa ggacggcgtg atgtatgtgc aggcaggcgg cggggtggtt gcggattccg
    10441 atcccgaggc cgaattccag gagaccgtca acaaggcaaa ggccctgatg gcagccgccg
    10501 acgaagccgt gcgctacgcc tcggcgccca agcgcgggcc gtaatctgtc cctgcccggt
    10561 gccgcggccc cgcgcggcgt tcccttcgcc gatccgccct gcgctcacga accgtggtgc
    10621 agcacgctca ggcctttttc ccgcagcggt gcgagcacag ccggcgccac gctctcatcc
    10681 cagcggtcca tcatgttcgt cgcaaattgg gcaatcggcg accaagccgg gtgctcgccg
    10741 ccgaaccaga tcatggccgc ataaaagacg agcgcggcga gaacgaacca ggacaacgaa
    10801 ctgctgtgct cggcgccctg cgctctaggc ggcctgctat gcgaactcat atgaacatgc
    10861 ttcgtcatga cgccatcacg ccgcatggcg agcgtgaagt caaaacactc cgccaagcaa
    10921 cgtcgccgaa tgctgagtac cgcggataaa attcaacgcg aatgcaatca tgacaaaatg
    10981 cgcaagcaaa gagccagacg tttatgacgt tattgcgaat aatgccgttc aaatgcgagg
    11041 gggtggactg tccgtgtcca gccccctccc gaaagagacc cggctagttg gcgttggcag
    11101 tacggatcgc ggtgaagccg tacatcacca acagatgggc cttgttggat tgcccatcac
    11161 cccattgctt gttcgccagc ggctccggat tcttcacctc gatgtcgaaa cgtcccgccc
    11221 gcttgagcag attgtcgtcg aggctgacct gcagctcggt cgcgttgatg cgtttgtatg
    11281 ggacggcccg gccgtcgaaa tagacccggc tgcggcgcac gaaattgaaa cctttcaatg
    11341 tgagcgtggt ggtggggctt ccttccgcga tcatcaccgg cgtgatcgcc tcgatggcgg
    11401 gctgcggatt ctccagcgga tcgggagctt cgcgcatcgc ggcttcgcct tggagcgcgc
    11461 ggaacgtcgc ctgcttcagc gattgcgtcc attctaaatt gtcgaccacc gcgcggtctt
    11521 cagccccgcc gccgaacggt gtgccgtacc acgcatgata gccaagctcg acaggcttgc
    11581 cgtcgaacac caccgtgctc acctttcgca aattcgcgac gtcggcgagc ggatcggcat
    11641 cgaccacgat gacatcggcg attttgcctg tttccaccgt gccgatcttg tcggcgacat
    11701 gcaacgcttc ggcggtccat ttggtcgcgc cctggatcac atgcatcgcg gggattccgg
    11761 cctccgccat cgtctccatc tcatcgtgca ggatgaagcc cggagctttc tggttgttgg
    11821 tatcgccacc ggcgagcaca tggcccccgg ctgcgtcgta catcctgttg aagcgcagca
    11881 tgttggcgaa cccgcgtgta cggcggtcgc gaacagcccc cgcgtcggac cgattatagg
    11941 tggcgaagaa actgcggata ttgccgtcgt cgtaataggc gcgcagatta gggtcctgaa
    12001 acgtcttgcg cgcctcggcc tggaaacgcg cccagccctt cggataaccc ggcgaatcgt
    12061 gcaccagggt cggcaccaga ttcacatggt gctcgaccag aaccttgatc aggtcgttgg
    12121 ctttcgcatc gtccatttcg gcgtagcggt cgagttcgtt gttggcgcgc gtgccatcct
    12181 tggcgacggc tgccccgatt cccgtggaat gggggaacac gtcggcaccg gcgagcgcgc
    12241 cttccttcgg accggtcttg ggtccaccac aacggcaaaa ggcgggtttt ccggctttgt
    12301 gcgcttcgtc gaaggcggtt tgatagattt ccggcgcgta ggaaccgtca tgcagcatca
    12361 ccatgtcggc gccggccgcc aggaagcgcc gcgcgaaggc ccgcgcatcg tcgggcgatt
    12421 tgggatcctt gcgtgtggca agtgccgatt ccagtccggt cggcatggcg ccgccgcgaa
    12481 tgccgccaag gtgaccaatt ccgaccatct gccgcggccc gatggccttg ccgtgattca
    12541 ccgcatcgcg ctgtgcgatg gaaacttcct caccatcacc gatatcgaca gtggaggtca
    12601 cgccctgatt gagaaatact tcgccccagt accaggcgat attggtctgc gcgtcccaca
    12661 gacccggcag cacgtatttg ccactcgcat tgatcgtctg cgcgccagcc ggaaccggta
    12721 ccgcgcccgc acgtcccacc gcgaggatac gattgccctg gatcaccacg acggaattcg
    12781 cgaccggcgc accgccattg ccatcgatca gcgtgccccc ttggatgacg atcgcaggac
    12841 caccggcgtt ctgagcggcc accttgaccg gtccaacgcc tatcgccagc cccagacaag
    12901 cagcgacgga aatcccgcaa agaagcttct tcatgacata tctccccaga ggcgctgtgc
    12961 gcggccattt tcggttccca tcataggacg ggtcgcgcgc gatctcatat gacttgttgg
    13021 aaaggctttt tcccgcaaga aagccgcctt caccggcggt acaacccaag aagccttgct
    13081 aaagcattgt tttcacatat taaatgaagg gcttacgttc ctgctgcgtt tgcgcgaaaa
    13141 tgagcttcga aacaggcgcg ctcccgtggc gcgcggacac accgaggaat acgcggctcc
    13201 a
//
LOCUS       DASYEG010000131        28601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3085534, whole genome shotgun sequence.
ACCESSION   DASYEG010000131 DASYEG010000000
VERSION     DASYEG010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 28601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28601
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3085534"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VGM72_05565"
     CDS             complement(<1..267)
                     /locus_tag="VGM72_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264765.1"
                     /translation="MDRFCRDCFADVPHDGVRCPGCHGERILFHAELVALAIAHLDCD
                     AFYASVETRDDPSLKGKPLIVGGGKRGVVLTASYEARKFGVKSAM"
     gene            404..859
                     /locus_tag="VGM72_05570"
     CDS             404..859
                     /locus_tag="VGM72_05570"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEY4264766.1"
                     /translation="MFDQILKLLGSPEPKSAHRDDLQIAVAVLLVEAARRDDTFDAIE
                     RAAIERLLSDKFALSPDATRQLLAQAEATADRTSQLHPFTRLAVERMDPQQRIRLIEM
                     LWEVAYADGILDPEEDALVRRVAGLIYVSDADRVAARQRVLERIQRKSH"
     gene            914..5263
                     /locus_tag="VGM72_05575"
     CDS             914..5263
                     /locus_tag="VGM72_05575"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY4264767.1"
                     /translation="MTPTELFHPAVAAWFDKSFAGPTAAQAQAWPAIRAGENVLIAAP
                     TGSGKTLAAFLAAIDALVRRGLEGGLPDETQIVYVSPLKALSNDVQRNLDVPLAGIAE
                     ELRARSLPDVEIRTWVRTGDTPQSERSAMRKRPPHIVVTTPESLYILLGSESGRAMLK
                     TTKTVIVDEIHALAPNKRGSHLSLSLERLAALCEHRLLRIGLSATQKPIEDVAHFLVG
                     ADPGATCKIIDTGHVRARDLALELPPAPLEAVMSNEVWQQIYDRLVQLIDTHRTTLIF
                     VNTRRLVERVTAQLSDRLGETAVAAHHGSLAKEQRLDAEQRLKHGKLKALVATASLEL
                     GIDIGDVDLVCQIGSPRSIAAFLQRVGRSGHAVAGTPKGRLFPLSRDELVECTALIAA
                     VHRGELDRLAIPESPLDVLSQQIVAELTAREWSEDELFALLRRAWPYRRLPREDFNAV
                     VTMLAEGFTTRRGRRGALIHHDAVNHMLRARRGARLTAITSGGAIPDNADYKVLLEPE
                     NNFIGTVNEDFAVESLAGDVFQLGNHSYRIMRVERGTVRVEDAHGQPPTIPFWLGEAP
                     GRSDELSQSVSRLRTDLAAHFSADPSGENAQAWLTQDIGLAETPARELSDYLRAGHAA
                     LGALPTQDIIILERFFDEAGGMQLVVHSPFGSRINRAWGLALRKRFCRKFNFELQAAA
                     TEDNIILSLTTAHSFDLADVARYLNSASIETLLIQALLDAPMFATRWRWVIGISLAEP
                     RFRGGKKIAPQLARMAAEDLLGAVFPDQIACAENLVGEREIPDHPLVNQTIADCLSEA
                     MDLAGLIRVLKRIESGDIRIVTCDLTEPSPLAQEVLSARPYSYLDDAPLEERRTQAVM
                     SRRWTAPEAASDLGRLDAEAIARVRAEAWPEAVNADELHDGLVWLGFLTSEEVAAQPG
                     WTEWLGELSLQNRVARMQLPRGNIWVPAERLPQFLSVWPTATLHPAIAVPETYARKWP
                     PEEALVEILRGRLEGLGPVTQEALAAALGFSASDIAIALVSLESEGFAMRGRFTPGAE
                     TEEWCERRLLARIHGYTVKRLRAEIQPVPARDYLRFLASWQRVSPDTRMEGPDALDTI
                     IHQLEGFEAPAAAWEAEILRARIRGYEPNWLDDRCLAGHVTWTRLRPRANSGEAKPAP
                     VRTTPIALLARRHARLWTMLSPTGDDIPLSPHAETVAGFIREHGASFFDEIFDGTGLL
                     RIQVEGALAELVAQGLVNSDSFGGLRALLVPASERKSPSGRRRRAVKFGMEDSGRWAL
                     IRRTHSAPQAEADAVEHVARTLLQRYGVVFWRLLAREAEWLPPWRDLLRVFRRLEGRG
                     EIRGGRFVAGFSGEQFALPEAIGALRDMRRQEPTGAWVSVSGADPLNLAGILTPGPKL
                     SALTGNRLLYRDGLPIALLTGGEVQFLETLEASDEWQAKKVLLRGASPSPEDVDELDQ
                     RRNRRRPLLDAAQ"
     gene            complement(5276..6967)
                     /gene="ggt"
                     /locus_tag="VGM72_05580"
     CDS             complement(5276..6967)
                     /gene="ggt"
                     /locus_tag="VGM72_05580"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY4264768.1"
                     /translation="MLKTIAAASLTLGLVTTALAQDRSQSRSMVGSQAGVVASESVLA
                     SEVGARILESGGNAIDAAIATNAVMGLVAPMNDGIGGDLFAIVYEAKTGKLYGLNASG
                     WAPAALTADYLLGKGIKTMPQRGINSVTVPGAVEGWDQLSKRFGTKKFADLLAPAIAI
                     AESGFPVGEVVQAYWRDSEKMLKEDEPATKIYMPFGHVPAVGEVFKNPDLAATYRQIA
                     AGGRDAFYKGEVAKKILATEAANGGVMKAPDLAEYSAEWVEPISTTYHGWTVFELPPN
                     GQGIAALEMLNIMEKFPLAEMGHNSVKALHTMIEAKKIAYADMIRYDADPRFAKIPVA
                     GLKSKAFADSRAKLIDPEHAACDVGPGVPPETDKGTTYLSAVDKDGNMVSLIQSNYST
                     VGFGSGVGVSGAGFVLQNRGGLFTLDKSHPNVLAGRKRPVHTIIPAFMQKGDVRIAFG
                     IMGGWNQAQAHAQFVSNMVDFGMNVQGAMDAARFSKETFAGCDVNFEARIPELVRKEL
                     TAMGHKIIMRGDYSSTRMGSGQAVMRDFAKRVNFGASDPRKDGAAVAELQAFAKP"
     gene            complement(7184..7417)
                     /locus_tag="VGM72_05585"
     CDS             complement(7184..7417)
                     /locus_tag="VGM72_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264769.1"
                     /translation="MIARFASAEFLGANTSERLAKCRQMAEEASELAASATTEKQRAS
                     YLELVRQWGILAEEIERAGEPGQTGERNPQDGG"
     gene            complement(7438..7635)
                     /locus_tag="VGM72_05590"
     CDS             complement(7438..7635)
                     /locus_tag="VGM72_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264770.1"
                     /translation="MTDYRIYTLDSAEHIVRGVELQLSDDEEAVAAARQLVAGSPLEV
                     WSGARRVARIDPADLPSAHVR"
     gene            complement(7763..9844)
                     /locus_tag="VGM72_05595"
     CDS             complement(7763..9844)
                     /locus_tag="VGM72_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4264771.1"
                     /translation="MEHISYPCAGRARAILFAGTASSFCAVAALAAPEDSAAPAPTLI
                     APENNPAGESVYVWGLRENTIGQATSSSEGTVNFALFEDRPLLRPGELVEVIPGLAVT
                     QHSGSGKANQYFMRGFNLDHGTDFSVSFDGVPLNLPTHAHGQGYLDLNSITPEFIQVI
                     SYRKGTYFPDAGDFSAAGTATFETFSAPPAPYAETDVGKDNYYRLLGTTSLGKDGYIG
                     ADLTYSDGPWSSPENLRKVNLLGHFALGNDWTVTALAYTAKWNSTDQVPERAIDSGLI
                     TRLGAIDPSDGGKTSRFIGVVRNRNPDGWDAVAYVQKYDLKLWSNFTYFLDDPVNGDQ
                     FEQADNRWIFGGSVAKTWPNSLAGWSLTTGAQIRDDDIGKVALYRTDRRVEIGTVRSD
                     AVNEWAGALWANGEQAFGTVRVSLGLRLDVIGANVSSDNPLNSGSTSDSLLSPKLTLA
                     WQALPNLELYADAGRGFHSNDARGATETIAPKTGLPADPVELIAPALGAETGLRWNLG
                     GFTATATLFWLHLDSELTYSGDAGDTESSSATQRLGGEVLLDWHPIDRIDLDLSAATT
                     HARYLSHPADGDRIPNAIEYMVTGGISALITDRLSTTLTLRYLGPSPLTQDGLVRSKA
                     DLDTNFLIRYQWGRFTFTGEILNLLNNSDDDIEYFYTSRLQGEPAEGVDDFHIHPMEP
                     RTWRLGLRVAL"
     gene            complement(9959..10435)
                     /gene="nikR"
                     /locus_tag="VGM72_05600"
     CDS             complement(9959..10435)
                     /gene="nikR"
                     /locus_tag="VGM72_05600"
                     /inference="COORDINATES: protein motif:HMM:NF002815.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HEY4264772.1"
                     /translation="MQRVTITLDDEFVETLDRVIAERGYQNRSEAIRDLARAGIRQAA
                     EESDLSGDCVAALVYVYDHESRELAKRLTRVFHDHHDLTLSSMHVHLDHGSCMELVVL
                     RGQAGAVGHLADHVIAERGVRHGRVVMVPVAVENEKHPHGGKPSHGHMHVHVQKTG"
     gene            10563..11015
                     /locus_tag="VGM72_05605"
     CDS             10563..11015
                     /locus_tag="VGM72_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264773.1"
                     /translation="MVRGWSMCESHRFLLAAATAGMLFAACAASAAPAGAGGAASVTL
                     RCNLHEAGSDVPVIQYYVVDSGQKTVTSGGELYRIGADPSGATGRVITRWSDTEITMV
                     NEEWIRNGWQRFRIVTTLDRLTGAIRSEDLNISYSGNCTIADVAKKLF"
     gene            11069..12757
                     /locus_tag="VGM72_05610"
     CDS             11069..12757
                     /locus_tag="VGM72_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY4264774.1"
                     /translation="MSVEHFQALAKGVRLNQYEMLGILGAGGFGITYMARDTTLDALV
                     AIKEYLPGDFAVRQGDSQVTAKSSTSKGDFDWGLSAFLDEARVLAKFRHPNIVHVNQI
                     FEANNTAYIVMEYAKGETLDALLKRAGTLKEPETKDILFPILDGLKRVHALGFLHRDI
                     KPANIIIREEGGAVLIDFGAARQAIETKSRAITSIVTEGYAPLEQYDTTGHQGAWTDI
                     YALGGVAYKCLTGNKPPPATSRLRNDPLIPLAIAAKKPVSKEFASAIETALQVFENQR
                     PQSIDEFAARISGIIPAGAPPSDAEDATRVMKPEAPRAASPVSQVPVSLAPVSLAPVS
                     PARDIPAAPPSASPVTPRRSMPGMLPAVLGLAASLLLALTAVGWLYLRGPSPSQTAAS
                     QSAATQSVAIQNAVTQSAGAVLPSPEIASVAPSPPVPSVAAPKPDVAANPPASRPAPS
                     PALPPHAEAPKERQAAQAIPAPPPAPAFRPSFDCAKAGNDAERLVCTDSQLAALDVTM
                     ADLYQLGLKSVTDTNEFRGEQVGWLTQRDVCADKQCLVVSYNDRIKDLQRWVRH"
     gene            complement(12793..13557)
                     /locus_tag="VGM72_05615"
     CDS             complement(12793..13557)
                     /locus_tag="VGM72_05615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM/exosortase system-associated
                     acyltransferase"
                     /protein_id="HEY4264775.1"
                     /translation="MFQNEAFNSTDDAPSTYSNSSVAEQSLLQCFQSHFMTVDADKPP
                     FVGAAYRLRYQIYCVEKQFEDTNAHPNELEHDEFDSHAVQGLLLHKDTGIALGTARLV
                     LPLSNAPEKSFAVQRMLREESTKIAASVPLHSTAEVSRFSISKQSIRRLSTTTNATPA
                     DYRTGPLMRLGLIQMLIRMSAKNGITHWYCMIEPSLKRMLDAMAFYMEPVGPLVDFHG
                     MRQPCFCSLSKVLAAMKLERPSFWDVLTEDGKFAIA"
     gene            complement(14269..15288)
                     /locus_tag="VGM72_05620"
     CDS             complement(14269..15288)
                     /locus_tag="VGM72_05620"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HEY4264776.1"
                     /translation="MRHAYAAKSAFGIAAAASIAFSVQSGFAQITPDPNSAPQTYRED
                     SGWAKLTGGRKWGAVSAVDTDRDGKSIWVFDRCETADDCSASNLDPIQKFDASGKIVF
                     SFGKGLFNYPHGIYVDAQDNIWVSDGRVVKNNGKGQTVMKFSPQGKLLMTLGTPGVAG
                     NDATHFNAPSDILVAPDGDIFVADGHGGDTNARIVKFDKTGKFIKTWGKHGTGPGDFD
                     APHGLAMDSAGRLFVADRSNSRIQIFDQDGKFLAEWRQFGRPSGVYINKNDIIYVADS
                     TSSDKVNPGYKQGIRIGSVKDGKVTAFIPWNETNTLEGVAADDAGNVYGGFTNTLNFR
                     RFVKK"
     gene            complement(15333..16031)
                     /locus_tag="VGM72_05625"
     CDS             complement(15333..16031)
                     /locus_tag="VGM72_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY4264777.1"
                     /translation="MNLSRRETIQSATAIAVLSVAAMRPGSAIAQTDAAQPDALVDTR
                     LRTIAPLPLRGDGSAVEYSQAEAGAIDGVLWKTKDTPDIEFDYRKMKVKVDGRGTSTL
                     SGTLSFGDLEKLPRHSQITLLQCGAVKPHGIVKWTGVRFSDFAASVGAQSFANYARFT
                     ASDDYYLDEDMKTLMHPQVMLAWLANDAPLPPQNGAPLRLVMPFRYGARSVKAITAIA
                     FTATSFPPQKAWAA"
     gene            complement(16204..16872)
                     /locus_tag="VGM72_05630"
     CDS             complement(16204..16872)
                     /locus_tag="VGM72_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEY4264778.1"
                     /translation="MCGKFTQMMGWGAHVRLADLIGNAGSASETVTPMRFATLIHLAA
                     DGQRQAARMRWGLVPPWAKDPNMASFIHARAESIDERPTYRDAFRHRRGILAVQTFNE
                     GKEITPHKTEQHVIAANDAAPIAIAVIWERWGEVRSGKGLETFAMVTVPPNRLIATIT
                     DRMPAVIQPHRWSRWLGEEPASVQALKDMLVPCEGDWTMRPAPQAGRARPSPDESDPQ
                     LPLL"
     gene            complement(17091..17645)
                     /locus_tag="VGM72_05635"
     CDS             complement(17091..17645)
                     /locus_tag="VGM72_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEY4264779.1"
                     /translation="MPDLAGRKIAYLGYTGPIDSEGVSRIATSLNVAVNGSADEAHLC
                     FSSLGGYVSDGIYLYNHIRSLPINVVIHNTGSVSSIAVTVFVAAHERYCSPHAMFMIH
                     PTVMPSQEGMTSERLQSSLNAALADDTRTESILRERAAIPEEILVARRFKDLYITPKE
                     AMIYGLVHAVKNFSLPKGHQILQI"
     gene            17879..18097
                     /locus_tag="VGM72_05640"
     CDS             17879..18097
                     /locus_tag="VGM72_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264780.1"
                     /translation="MGNDNMNRPSGQGQSGQGQSGQGKQQGQSGQGHGSQGQQGQGSQ
                     GQQGQGSQGQQGQTSKQGQRSGRSSSNE"
     gene            18266..19414
                     /locus_tag="VGM72_05645"
     CDS             18266..19414
                     /locus_tag="VGM72_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005262059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEY4264781.1"
                     /translation="MDFALGETADLLRQTVRGFAADHIAPRAEAIDRENTFPRDLWPK
                     LGGLGLFGVTVAEDYGGAGMGYVEQAIAVEEISRASASVGLSYGVHATLCINHLYRFG
                     TEEQKRRYLPQLLSGEWVGALAMSEPGAGSDVLAMTTRAELQNGRYVLNGTKMWITNG
                     PVADFLIVYAKTGDPSGRRGITAFLIEKSFKGFSTAQKLDKLGMRGSDTGELLFKDCE
                     VPGANVLGGVDKGLSVLMSGLDYERVVLSAGPVGIMQACLDLVLPYVRQRKQFGRAVG
                     EFELMQGKLADMDTTLNAARAYVYAVARACDSGTVTRKDAAGVILFTAEHATQMALQA
                     IQALGGMGYVNEMPAGRLLRDAKLYEIGAGTSEIRRMLIGRELFEGRE"
     gene            19414..20586
                     /locus_tag="VGM72_05650"
     CDS             19414..20586
                     /locus_tag="VGM72_05650"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY4264782.1"
                     /translation="MSAETIVIAGAARTPIGSLQGQFKSLSATALGAVAIGAALERSG
                     LDSDAPGEVFMGCVLSAGLGQAPARQAAIAAGIPYGVGCTTVNKVCGSGMKAAMLAHD
                     AILTGSANVVVAGGMESMTNAPYLLKKARGGYRLGHDAVYDHMFLDGLEDAYRPGTLM
                     GQFAEDTAEKYQFTRAVQDEFATLSLQRAKHAAAEGIFAREIAGVRVGDAEITEDELP
                     ARADAAKIPRLKPAFREGGTVTAANSSGISDGAAALVLMSQDEADRRNVRPLARIAGH
                     ASHAQAPALFTTAPVRAIQKLREKIGWNPDDVDLYEINEAFAVVALAAMRDLDIPRDK
                     INVHGGACALGHPIGASGARIVVTLLNALQEKNLKRGIASLCIGGGEATAMAVELI"
     gene            20588..22180
                     /locus_tag="VGM72_05655"
     CDS             20588..22180
                     /locus_tag="VGM72_05655"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY4264783.1"
                     /translation="MAVLTTRLDRASKEYAANARAMEALVDDLQARLARIRLGGGEAA
                     RNRHTERGKLLARARIAALLDKGADLLELSAFAADGVYDDPLPAAGLITGIGTIAGRD
                     CVVVVNDATVKGGTYYPLTVTKHLRAQDIAIENRLPCLYLVDSGGAFLPLQDQVFPGR
                     DHFGRIFYNQAQLSAAGIAQIAAVLGSCTAGGAYIPAMCEESIIVKGQGTIFLAGPPL
                     VKAATGETISAEELGGAETHARLSGVVDHVAKDDADAMRQMRGIIRNLPAPPATIAPK
                     EVHPPLYDAEELGGIVPADLRWIYDVREVIARIVDGSEFDEFKPLYGATLVCGFAHVH
                     GHIAGIVANNGILLSEAALKATHFIELCCQRGVPLIFLQNTSGFMVGRKAEAEGIAKH
                     GAKMVMAVANAQVPKITIIIGGSFGAGNYAMCGRAFSPRFLFLWPNARISVMGGEQAA
                     QVLSRIKREPGQTQPDAAQIARADEIRNQYATQGAAYYASARLWDDGIIAPAETRDVL
                     GRCLGVVGNAPKVPTKFGVFRM"
     gene            22183..23097
                     /locus_tag="VGM72_05660"
     CDS             22183..23097
                     /locus_tag="VGM72_05660"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEY4264784.1"
                     /translation="MRVPKSVRMVEVGPRDGLQNESAALSTALKVELIDRLSAAGLKT
                     IEAGSFVSSKWVPQMADTAMVLARIERAPGASYPVLVPNMKGFEAARAAGADEIAIFT
                     AASESFVRANINCSIGESFERFEPVCAAACASGMKIRGYVSCALGCPYEGEIAPARVA
                     AVTKRLLQFGCYEVSLGDTIGVGTPRKTQILLETVSAECPREKLAVHFHDTYGQGLAN
                     ILAALEMGIAVVDSSVAGLGGCPYAPGASGNVASEDVLYMLSGMGIQTGVDLGLLVET
                     GRFIAGAFHRPPGSKANIALSNRTSPSG"
     gene            23170..24258
                     /gene="pdhA"
                     /locus_tag="VGM72_05665"
     CDS             23170..24258
                     /gene="pdhA"
                     /locus_tag="VGM72_05665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEY4264785.1"
                     /translation="MNKHGMPGIERIVAEFQIRHRQCLDATGTLIGEPPAFAHDRDIM
                     IALYRAMTLTRIFDAKAIALQRTGQLGTYPSCAGQEATGIGYASAMAADDVMFITYRE
                     QAAQIWRGVTLTELLQFWGGDERGCNYAGPREDFPPAVPIASQATHAAGAATAFKLRH
                     EKRVAVCALGDGATSKGDFYESINLAGVWRLPAVFLVIDNFWAISLPREKQTATGTLA
                     QKAIAGGIPGIQVDGNDVIAVRAAMDEALARARAGEGASVIEAITYRMGDHTTADDAS
                     RYRREELVSAAWKLDPLARLRTYIANRHWWTKADEERLIGESREKVDAAVADYLAIAP
                     PAPESMFDHLFETLPASLQWQRDRMARR"
     gene            24260..25240
                     /locus_tag="VGM72_05670"
     CDS             24260..25240
                     /locus_tag="VGM72_05670"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018392213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY4264786.1"
                     /translation="MAEITMVGAIAMALARSLAGDSRVLVLGEDVGANGGVFRATDGL
                     QQRFGEERVMDTPLAETVLAGMSIGLAAQGFRPVTEFQFMGFIYPALDEVINHAGRLR
                     NRTRGRLSCPLVFRAPMGAGIHAPEHHSESTEALFAHIPGIRVVIPSSPARAYGLLLS
                     AIADPDPVMFFEPTRLYRAAKEEVGDNGQGLPLDVCFLLREGADVTLVTWGAAVKETL
                     AAAELLGAEGISAEVVDVATVSPLDFETILASVEKTGRLVIVHEAPRNCGIGAEIAAR
                     MAERGLLSLQAPIERVTAPDVVVPLLRLEKAYMPSEQDIVEAARRAVSFQ"
     gene            25237..26367
                     /locus_tag="VGM72_05675"
     CDS             25237..26367
                     /locus_tag="VGM72_05675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEY4264787.1"
                     /translation="MRTFLLPDLGEGLSEAEIVSWHVSPGDHVVTDQPLVSVETDKAV
                     VEVPSPYSGTVAALHAAAGDIVRIGAKLADFDEAGAHEDRGTVVGAVPPAESSTTGQT
                     AERTPVPGATLRAMPVVRALARKLGVDLAGVEPSGPNGAVTAEDVTRYASAMSDAGPG
                     EPLHGVRRAMAQKMEQSHAEIVPASVYDEADVEDWVEGGDVTVALIRALVAGCRVSPI
                     LNAWYNGADKMVRIVGKIHVGMAINTADGLFVAVMRDVAGRDDTDLRQGLNNLKRAVQ
                     ARRIPLEELRGATITLSNFGVSGAGRFANLIVVPPQVAIVGAGTIKPRIVARAGAPAV
                     RRTLPLSLTFDHRVVTGIEAANFLKSMVTALETRPVTVLTRQ"
     gene            26419..26865
                     /locus_tag="VGM72_05680"
     CDS             26419..26865
                     /locus_tag="VGM72_05680"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY4264788.1"
                     /translation="MNDKSPLPFTKRERRSADRRQKRRPAVELRGEIRMGRKSWPVLI
                     GEIPGSGALVFTDSPPPVGNAVELWIEGFGAVPVEVVHSSRRLCGLVLCDPAAYRAEL
                     LLWLRRMLKTKTSAQPKDRRTPLFFCARVAELASYRSALSPSHQTR"
     gene            complement(26862..27953)
                     /locus_tag="VGM72_05685"
     CDS             complement(26862..27953)
                     /locus_tag="VGM72_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264789.1"
                     /translation="MGTDAQTPNRDVPATGTGGSRLRRWAKSITAVVAVLGVVSATIG
                     YINNGANFFSHVTEYFQGQSEVRTLTQTADERLAHKDYEAAWTANAKARQIAPRNAEA
                     SAQQARVAMRWLEDVHTGSANGPRTFSSIVDPLKAALIEHLPGTEDAEKADLEAHIGW
                     ANFLRSRDGNPETDITEEFNAAIADDSANMYGHVMRGFWILWKGGPVDKARPDLDVAL
                     ASFSDPAFSDSMIMAGLTNNTSSPFIAGAIEYADKIRVAGRNIDDHTKGTLLWYYSVS
                     LHDTELMARISMTLPADKQIVFLDWLKQGDISDNYKRGVAYLTAHFTEAQGKKDEALG
                     LYKDLVGPKPDANEITGLAQAGVARLQKR"
     gene            complement(28283..>28601)
                     /locus_tag="VGM72_05690"
     CDS             complement(28283..>28601)
                     /locus_tag="VGM72_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264790.1"
                     /translation="NALGTLAGCLYIPTLMTAVYNQAKRSPCALRFHVATEGGWDIGC
                     AAGCLAVAALSWAGAPLPVGILLSLAGTVLSLVLLRRYYAGLGVPRDLMIVPPPGGAL
                     REP"
BASE COUNT         5281 a         9041 c         8972 g         5307 t
ORIGIN      
        1 catcgcggac ttgacgccga atttgcgcgc ctcgtagctg gccgtgagca caaccccgcg
       61 tttgccgcct cccacgatca gcggtttgcc tttcagcgag ggatcgtcgc gcgtttccac
      121 gctggcgtag aaggcgtcgc agtccagatg cgcaattgcg agcgcgacga gttcggcgtg
      181 gaaaaggatg cgttcgccat gacagcccgg gcagcgcacc ccatcatgcg gcacatcggc
      241 gaaacaatcg cggcaaaacc tgtccatgcg aatcctgggg taagccgccc taatatcgcc
      301 gattttcggg agggttgtgc actcccgtta ttgtttcctc ggcttcatta gcgggaggcg
      361 ggaatgcggg ttatgtatcc ctcgcaaaaa tcaggtcttt tccatgttcg atcaaatcct
      421 caaactattg ggctcaccgg agccaaaatc cgcccatcgc gacgatttgc agatcgccgt
      481 ggcggttctg ctggtggaag ccgcgcggcg ggacgacacg ttcgacgcaa tcgaacgggc
      541 ggccatcgag cgcttgctga gcgacaaatt cgcgctgtcg ccggacgcga cacggcaatt
      601 gctggcgcaa gccgaagcca ccgcggaccg cacgtcccaa ctccatcctt tcacgcggct
      661 cgccgtcgag cgcatggacc cgcagcagcg catacgcctc atagagatgc tgtgggaggt
      721 ggcctatgcg gacggcattc tcgatcccga ggaagacgcg ctcgttcgcc gggtcgcggg
      781 actgatttac gtgtcggatg ccgacagggt tgctgcgcgt cagcgcgtcc tggagcggat
      841 ccagcgaaag tcgcactagg ccgaactcag gttgatccgg ctggccgcgg gccccatatt
      901 gccgataccc aaaatgaccc cgaccgaact gtttcatccg gccgtcgccg cctggtttga
      961 caaaagcttc gccggcccca cggcggcgca ggctcaagct tggccggcga ttcgagcggg
     1021 cgagaatgtt cttattgcgg cgcctacggg gtcgggcaaa acactcgccg cgttcctcgc
     1081 ggccatcgat gccctggtcc ggcgaggtct agaaggtgga ctgcccgacg aaacgcaaat
     1141 cgtctacgtg tcaccgctga aggcgctctc caacgacgtt cagcgcaatc tggacgtacc
     1201 gctcgccggc atcgccgagg agctgcgggc gcgatctctg ccggatgtcg aaatccgtac
     1261 ctgggttcgc accggcgaca caccgcagag cgagcgtagc gctatgcgca aacgtccgcc
     1321 ccacatcgtc gtgaccacgc ctgaatcgct ttacatcctg ctcggctccg aatcgggccg
     1381 cgcgatgctg aagacgacga agaccgtcat cgtcgacgaa atccacgctc ttgcccccaa
     1441 caagcggggc tcgcatctgt cgctgtcgct cgaacgcctg gcggcgctct gcgaacaccg
     1501 gctcctgcgc atcggcttgt cggcgacgca gaagcccatc gaagatgtcg cgcatttcct
     1561 ggtcggcgcc gatcccggcg cgacgtgcaa gatcatcgac accggccatg tgcgcgcccg
     1621 tgacctcgca ctcgaactac cgcccgcccc tctcgaagcg gtgatgtcga acgaggtttg
     1681 gcagcagatc tatgaccggc tcgtgcagct catagacacc catcgcacca cgctgatttt
     1741 cgtcaacacg cgccgcctcg tcgaacgcgt gacggcccag ctttccgacc ggttgggcga
     1801 aacggccgtc gccgcccacc atggcagcct tgcgaaggaa cagcgcctcg atgccgaaca
     1861 gcgcctgaaa cacggcaaac tcaaagcgct ggtggcgacg gcttcgctgg aactcgggat
     1921 cgacatcggc gatgtcgatc tggtctgcca aatcggttcg ccccgttcga ttgcggcgtt
     1981 cctgcagcgt gtcggacgct cgggccatgc cgtcgcgggc acacccaaag gccggctgtt
     2041 cccgctctcg cgcgacgagc ttgtcgaatg cacggcgctg atcgccgcgg tccatcgcgg
     2101 cgaactcgac cggctcgcca ttccggaaag tcccctcgat gtgctgtccc agcagatcgt
     2161 ggccgagttg acagcgcgcg aatggagcga ggacgagctc ttcgcgctcc tgcgccgcgc
     2221 ctggccctac cggcggctac cccgcgagga tttcaacgcc gtcgtgacca tgctggcgga
     2281 aggttttacg acgcgccggg gacggcgtgg cgcgctgatc catcacgatg cggtcaatca
     2341 tatgctgcgg gcgcggcgtg gcgcgaggct gacggccatc acatcgggcg gcgccattcc
     2401 cgacaacgcc gattacaagg tcctgctgga gccggagaac aatttcatcg gcaccgtgaa
     2461 cgaggatttc gcggtcgaga gtctcgcggg cgatgtgttc cagctcggca atcattccta
     2521 tcgcatcatg cgggtggaac gcggcacggt gcgcgtggaa gacgcccatg gccagccgcc
     2581 gacgattccg ttctggctgg gcgaggcacc gggccgcagc gacgaactct cccaatcggt
     2641 gtcgcgcctg cgtaccgatc tggccgcgca tttctccgcc gatccgtcag gcgagaatgc
     2701 gcaagcctgg ctgacgcaag acatcggcct tgctgaaacg ccggcgcgtg aattgtcgga
     2761 ttatctgagg gcaggtcatg ccgctttggg cgcgttgccg acgcaggaca tcatcattct
     2821 cgaacggttc ttcgatgaag ccggcggcat gcagctcgtc gtgcactcgc ccttcggcag
     2881 ccgcatcaat cgcgcctggg gtttggcctt gcgcaaacgc ttttgccgca aattcaattt
     2941 cgagctgcag gcggcggcca ccgaggacaa catcatcctg tcgctgacca ccgcgcacag
     3001 tttcgatctt gccgatgtcg cgcgctatct caattcggcc agcatcgaga ccttgctgat
     3061 tcaggcgctg ctcgacgcgc cgatgtttgc cacgcgctgg cgatgggtga tcgggatatc
     3121 gctggccgag ccgcgttttc gcggcggcaa gaaaatcgcg ccccagctcg cccgcatggc
     3181 ggcggaagat ttgttgggag cggtgttccc cgatcagatc gcttgcgccg agaatctcgt
     3241 cggcgagagg gagattcccg atcatccact ggtcaatcag accatcgccg attgcctgag
     3301 cgaagcgatg gatttggcgg gtctgatccg ggtgctgaag cgcatcgaat ccggcgacat
     3361 tcgcatcgtc acctgcgatc tcaccgaacc ctcgccgctg gcgcaggaag ttttgtccgc
     3421 gcggccctat tcctatctcg acgacgcgcc cttggaggaa cgccgcaccc aagccgtgat
     3481 gagccggcgc tggaccgcgc ccgaagccgc atccgatctc ggccgcctgg acgccgaagc
     3541 gattgcgcgg gtgcgcgccg aagcctggcc ggaagcggtc aacgcggacg agttgcacga
     3601 tgggctcgtc tggctgggct ttctcacgag cgaagaggtt gccgctcaac ccggctggac
     3661 agaatggctg ggtgagttgt cgcttcagaa ccgcgtggcg cggatgcaat tgccgcgtgg
     3721 aaacatctgg gtaccggccg agcgcctgcc gcaattcttg agtgtctggc caacggccac
     3781 gctccatccc gcgatcgcgg tacccgagac ctacgcgcgc aagtggccgc ccgaagaggc
     3841 actggtggaa atcctgcgcg gacgactcga gggtctgggt ccagtcacgc aagaggcgct
     3901 ggctgccgcg ctgggcttct ccgcgagcga tatcgccatc gcactggtgt cgctcgaatc
     3961 cgaaggcttc gccatgcgcg gacgtttcac gcccggcgcc gaaaccgagg aatggtgcga
     4021 gcgccgtctg ctcgcgcgca ttcacggcta cacggtcaag cgtttgcgtg ccgaaatcca
     4081 acccgtgcct gcgcgcgatt acttacgctt cctcgcgtcc tggcaacgcg tctcgcccga
     4141 tacgcggatg gaagggcctg atgcgctcga caccatcatc caccagcttg aggggttcga
     4201 ggcgcctgcc gccgcctggg aagcggaaat cctgcgggcg cgcatcaggg gttatgagcc
     4261 caattggctg gacgatcgct gccttgccgg ccacgtcacc tggacgcggc tgcgcccgcg
     4321 cgcgaacagc ggcgaagcaa agcccgcgcc cgtgcgcacg acacctattg cgctcttggc
     4381 acgccgtcac gcgcgcctat ggacgatgct gtccccgacg ggagacgaca tcccattgag
     4441 cccgcatgcc gagaccgtgg ccggtttcat ccgtgagcac ggcgcctcct tcttcgacga
     4501 gatcttcgac ggcacggggc ttttgcgcat ccaggtcgag ggggcactgg ccgagctggt
     4561 ggcgcagggt cttgtcaatt cggacagttt cggcggcttg cgcgcgctgc tcgtgcccgc
     4621 cagcgaacgc aaatcgccat cgggccggcg ccggcgcgcg gtcaaattcg gcatggagga
     4681 ttcgggccgc tgggcgctga tccgccggac ccattccgcg ccgcaggccg aagcagacgc
     4741 ggtcgagcac gtcgcgcgca cactgttgca gcgctatggc gtggtgttct ggcgcttact
     4801 ggcgcgcgaa gccgaatggc tgccgccctg gcgcgatctg ctgcgcgtct tccgccgcct
     4861 cgaagggcgc ggggaaatcc gcggcgggcg cttcgtggcc ggattttccg gcgagcaatt
     4921 tgcgctgccc gaggccatcg gggcgttgcg ggacatgcgg cgccaggaac ccaccggcgc
     4981 gtgggtttcg gtgtccggtg ccgatccgct caatctcgcc ggcatcctga cgccggggcc
     5041 gaaattgtcg gcgctgacgg gaaatcgcct gctctatcgc gatggcctgc cgattgcgct
     5101 gctcacaggc ggggaggtgc aattcctcga aacgctcgaa gcgagcgacg aatggcaggc
     5161 gaaaaaagtg ctgctgcgcg gggcgtcccc ctcgccggag gatgtggacg aactggatca
     5221 gcggcgcaac cggcgccgcc ccctcctcga cgcggcccaa taatcatcat cgcaatcagg
     5281 gtttggcgaa cgcctgcaat tcggccacgg ccgcgccgtc tttgcgcgga tcggaggcgc
     5341 cgaagttcac gcgcttggcg aagtcgcgca tcaccgcctg ccccgagccc atgcgcgtgg
     5401 aggaataatc tccgcgcatg atgatcttat ggcccatcgc ggtcagttcc ttgcgcacca
     5461 gttcgggaat gcgcgcttcg aaattcacgt cgcagccggc gaacgtctcc ttcgaaaaac
     5521 gcgccgcatc catcgcgccc tgcacattca tgccgaaatc caccatgttg gagacgaatt
     5581 gcgcatgcgc ctgagcctgg ttccagccgc ccatgatgcc gaaggcgatg cgcacatcgc
     5641 ccttctgcat gaaagcggga atgatggtgt gcacgggccg cttgcgcccg gcgagcacat
     5701 tgggatggga cttgtcgagc gtgaacaacc cgccgcgatt ttgcagcacg aagcccgcac
     5761 cgctcacgcc gacgcccgat ccaaatccga ccgtcgaata attgctctgg atcagcgaaa
     5821 ccatattgcc gtccttatcg acggcggaca ggtacgtggt gcccttatcg gtctccggtg
     5881 ggacgcccgg cccgacgtca caggccgcgt gttcgggatc gatcagtttg gcgcgggaat
     5941 cggcgaacgc cttcgacttc aggcccgcaa ccggaatctt cgcaaagcgc ggatcggcgt
     6001 catagcggat catatccgca taggcgatct tcttcgcctc gatcatcgtg tgcagcgcct
     6061 tgaccgaatt gtgccccatc tcggccagcg gaaatttctc catgatgttg agcatttcga
     6121 gcgcggcgat accctgcccg ttcggcggca attcgaacac cgtccagccg tgataggtcg
     6181 tcgaaatcgg ctcgacccat tcggcgctgt attcggcgag atcgggcgcc ttcatcacgc
     6241 cgccattcgc cgcttcggtc gcgagaattt ttttcgccac ctcgcccttg tagaaggcat
     6301 cccgcccgcc cgccgcgatc tgacgatagg tggccgccag atcgggattc ttgaacacct
     6361 caccgaccgc cgggacatgg ccgaacggca tataaatctt cgtggccggc tcgtcttcct
     6421 tcagcatttt ttcggaatcg cgccaatagg cctgcaccac ctcgccgacg ggaaagccgc
     6481 tttccgcgat cgcgatcgcg ggagcgagca gatcggcgaa tttcttggtg ccgaagcgtt
     6541 tggaaagctg atcccagccc tccaccgcgc ccggcaccgt caccgaattg atcccccgct
     6601 gcggcatcgt cttgatgccc ttgccgagga gataatccgc ggtgagcgcc gcaggcgccc
     6661 agcccgaggc gttgagacca tagagcttgc cggtcttggc ttcgtagacg atggcgaaaa
     6721 ggtccccgcc gatgccgtca ttcatcggcg cgaccaggcc catcaccgcg ttggtcgcga
     6781 tggccgcgtc gatggcgttg ccgcccgatt ccagaatgcg cgcgccgacc tccgaggcca
     6841 gcaccgattc gctcgcgacc acaccggctt gcgaccccac catggagcgc gattgcgaac
     6901 gatcctgcgc gagggcggtg gtgacgaggc ccagcgtgag gctcgcggcg gcgattgtct
     6961 tcagcatgaa atctttccca tgggtgggtt caggtcccgt gcgagaccct tggatgacgg
     7021 cgccggagca tagccatcgg gctcagggaa ttccacaccg gtggcttggc tctatggccg
     7081 gttcggggac ggattgccgt cattcccgcc ccccttattt ttgccggcgg ggcgacgaaa
     7141 aacccagttt tctcgaggat tcagggattg ccgccaggcg ccctcagccg ccgtcttgcg
     7201 gatttcgctc gccggtttgg ccgggttccc cggcgcgctc gatctcctcg gcgagaatgc
     7261 cccattgccg cacaagctcc aaatagctcg cgcgctgctt ctcggtggtt gcactggcgg
     7321 caagttcgga cgcctcctcg gccatctggc ggcacttcgc gagacgttcg gatgtattgg
     7381 cgccgagaaa ttccgctgaa gcgaatcgtg caatcatcgc cgcatgccac gccttaacta
     7441 acgtacgtga gcggaaggca aatccgccgg atctatgcgc gcgacacgcc tggcgcccga
     7501 ccagacttcc aatgggctgc ctgccacgag ttgcctcgcc gcggcgacgg cctcttcatc
     7561 atcggaaagt tgcagctcga caccgcgcac aatgtgttcg gcggaatcca gagtgtagat
     7621 tcgataatcg gtcatgtcgc ctgcatgctt taaccatgtt tactatccgg ccatcgctag
     7681 catgaagcga gtcgaatcat ggttcgctag cgtacggtcc cggttgcaaa ttgcggaacg
     7741 cggcggcgcc gattccactc cgctaaagcg ccacccgcag gccgaggcgc caggtgcgcg
     7801 gctccatggg atggatgtgg aaatcgtcga cgccctcggc gggctcgccc tgcagacgcg
     7861 aggtgtagaa atattcgata tcgtcgtcgg aattgttgag tagattcaga atctcccccg
     7921 tgaaggtgaa gcggccccac tgataccgga tcaggaaatt ggtatcgaga tcggctttcg
     7981 aacgcaccaa gccatcctgc gtcagcggtg agggccccaa atagcgcagt gtcagcgtcg
     8041 tactcaaacg gtcggtgatc agcgccgata tgccgcccgt caccatgtat tcgatggcgt
     8101 tcggaatgcg gtcgccatcg gccggatggc tgagatagcg cgcatgggtc gtggccgcgg
     8161 aaagatcgag atcgatacgg tcgatggggt gccaatccaa cagcacctcg ccaccgaggc
     8221 gttgcgtggc ggacgagctt tccgtgtctc cggcatcgcc cgaataggtc aattccgaat
     8281 ccagatgcag ccagaacagg gtcgccgtgg ccgtgaagcc gccaagattc cagcgcagtc
     8341 cggtttccgc gcccaaggcc ggtgcgatca gctcgacggg atcggcaggc aaacccgtct
     8401 tcggtgcaat cgtttcggtc gctccgcgcg catcgttgga atgaaatccc cgtccggcgt
     8461 cggcatagag ttcgagattg ggcagggcct gccaggcgag cgtcaatttg gggctgagca
     8521 gcgaatccga ggtcgaaccg gagttcagcg gattgtcgga cgacacattg gcgccgatga
     8581 catccagacg cagaccgagc gaaacccgca ctgtcccgaa ggcttgctcg ccgttcgccc
     8641 acaacgcccc cgcccattca ttcaccgcat cggagcgcac ggtcccgatc tcgacacgtc
     8701 gatcggtgcg atacagcgcc accttgccga tatcgtcgtc gcggatttgc gcgccggtgg
     8761 tgagcgacca tcccgccagc gaattcggcc aggtcttggc aaccgaaccg ccgaagatcc
     8821 agcgattgtc cgcctgctcg aactggtcgc cgtttaccgg gtcgtcgagg aaataggtga
     8881 agttcgacca caatttcaga tcgtatttct gaacataggc cacagcgtcc cagccatcgg
     8941 gattgcgatt gcgaacgacg ccgatgaatc gggacgtctt gccgccgtcc gacggatcga
     9001 tggcgcccag ccgggtgatg aggccggaat cgatggcgcg ctccggcacc tgatcggtgg
     9061 aattccattt cgccgtgtag gcaagagccg tgacggtcca atcattcccg agcgcaaaat
     9121 ggcccagcaa gttcaccttg cggagatttt ccgggctgga ccatgggccg tccgaatagg
     9181 tcaaatcggc cccgatatag ccgtcctttc ccagactggt ggttcccagc aaacgataat
     9241 aattgtcctt tccgacatcg gtctccgcat agggagccgg aggcgcggaa aaggtctcga
     9301 acgtcgcggt accggcagcc gagaaatcgc cggcatcggg aaaataagtc cccttgcgat
     9361 aggaaatgac ctggatgaat tccggcgtga tggaattcag atcgagataa ccctgcccat
     9421 gcgcatgcgt cggcagattg agcggcaccc cgtcgaagct cacggaaaaa tccgtcccgt
     9481 gatcgagatt gaagccgcgc atgaaatatt ggttggcttt gcccgagccg gagtgctgcg
     9541 tcaccgccag accgggaatg acttccacga gttcgcccgg cctcagcagc ggccggtcct
     9601 cgaacagcgc gaaattgacg gtcccttcgc tggaggaggt ggcttggccg atggtgttct
     9661 cgcgcaggcc ccacacgtaa accgattcgc cggcggggtt gttttccggg gcgataaggg
     9721 tcggagccgg cgccgcggaa tcttcaggcg cggcgagcgc cgcaaccgcg cagaaagacg
     9781 acgcggtgcc cgcaaatagg atcgcgcgcg cgcgacccgc gcacggatag gatatgtgct
     9841 ccacgacagt cccccaaagt atgatgtcta taacaaaaca tcatacatat cgcatgaccg
     9901 tcggctaggg aaaatatgac atcgctataa aaatatcata ccgcaaacgg catggggctt
     9961 aaccggtttt ctgcacatgc acatgcatgt gcccgtggct cggcttgccg ccatggggat
    10021 gtttctcgtt ctccaccgcg accggcacca tgacgacgcg tccgtggcgc accccgcgct
    10081 cggcgatgac gtgatcggcg aggtgcccga ccgcgcccgc ctggccgcgc agcaccacaa
    10141 gctccatgca gctgccatga tccagatgca catgcatgga agacagcgtg agatcgtgat
    10201 gatcgtggaa gacacgcgtc agccgtttcg ccaattcgcg cgattcatgg tcgtagacat
    10261 aaaccagggc cgcgacgcaa tcgccggaaa gatcgctttc ctccgccgcc tggcggatgc
    10321 ccgcacgcgc caggtcgcga atggcctcgg aacgattttg atagccccgc tcggcgatta
    10381 cgcggtcgag cgtttccacg aattcgtcat cgagggtgat ggtgacgcgc tgcatgggca
    10441 acctccaaag ccggaccaga ccccatagca caccgatagg gccggaaata ccgaagggca
    10501 tctgtcatgg agcggcgaac tttagtagga tcgcgaaaga ccagacttga cggaggggtg
    10561 ggatggttcg gggctggagc atgtgcgaat cgcatcggtt ccttttggct gcggcaacgg
    10621 ccggcatgct gttcgccgct tgcgctgcct ccgcagcccc ggcgggggcc ggcggcgcgg
    10681 catccgtgac gctgcggtgc aatttgcacg aagcaggatc ggatgtcccc gtcatccaat
    10741 attacgtcgt ggattcggga cagaagaccg tgacctcggg cggtgaactt taccgcatcg
    10801 gcgccgaccc cagcggcgca acggggcgcg tcattacccg atggtcggac acggagatca
    10861 caatggtgaa cgaggaatgg atccgcaatg gctggcagcg tttccggatc gtgacgacgc
    10921 tggaccggtt gaccggcgcc atccgcagcg aagacctcaa catttcctat agcggaaact
    10981 gcacgatcgc cgatgtggcg aagaaacttt tctagattcg cggatcaaac atcgattttc
    11041 tgcaaatcgc cgccacggaa aacgtgtcat gtcggtcgaa cattttcagg cgctggccaa
    11101 gggtgtgcgc ctcaatcaat acgagatgct cggcattctg ggcgccggag gtttcgggat
    11161 cacctacatg gcccgcgaca cgacgctcga tgcgctggtc gcgatcaagg aatatctgcc
    11221 cggtgacttt gccgtgcggc agggcgattc gcaggtcacg gcgaaatcca gcacctccaa
    11281 gggcgatttc gactggggtt tgagcgcctt cctcgacgaa gcgcgcgtgc tggcgaaatt
    11341 ccgccacccc aatatcgtgc acgtgaacca gatcttcgaa gccaacaata ccgcttacat
    11401 cgtgatggaa tatgccaagg gcgagaccct ggacgcattg ctcaaacgcg ccggtacgct
    11461 gaaggagccc gagaccaagg acatcctgtt ccccattctc gacgggctga agcgcgtgca
    11521 cgcactcggc ttcctgcatc gcgacatcaa accggccaac atcatcattc gcgaggaggg
    11581 cggcgcggtg ctgatcgatt tcggcgcggc ccgccaggcc atcgagacca agagccgggc
    11641 gatcacctcc atcgtcacgg aaggctatgc cccgctggag caatacgaca ccaccggcca
    11701 tcagggcgcg tggaccgata tctatgcgct tggcggggtg gcctacaaat gcctgacagg
    11761 caacaagccg ccccccgcca catcgcgatt gcgcaatgat ccgctgatac cgctggcgat
    11821 cgccgcgaag aagccggtct cgaaagaatt cgcttcggcc atcgaaacgg ccctgcaggt
    11881 cttcgagaac cagcgcccgc agagcatcga cgagtttgca gcgcgcattt ccggcatcat
    11941 ccctgctggc gcaccgccat cggacgccga ggatgcgacc cgcgtgatga aaccggaggc
    12001 tccacgagcg gcctcgcctg tttctcaagt gcccgtctct ttggcccccg tctctttggc
    12061 ccccgtctct ccggcgcgcg acataccggc ggcgccaccg tcagcatccc ccgtaacgcc
    12121 aagacgttcc atgccaggaa tgctccccgc cgtgcttggc cttgccgcct cccttctgct
    12181 cgcgctcacg gcagtgggct ggctctatct gcggggacca agcccgtccc agaccgcggc
    12241 gtcccaaagc gccgcgaccc agagcgtcgc gatccagaac gccgtgaccc aaagcgccgg
    12301 ggcggtcctg ccatcgcccg agatcgcatc ggttgcacct tcgccgcccg ttccaagcgt
    12361 cgcggcgccg aaaccggacg ttgccgccaa tccaccggca tcacgccccg cgccgtcgcc
    12421 ggcgctcccg ccgcacgccg aagcgccgaa agagcgccaa gccgcgcaag ccattcccgc
    12481 gcctccgcct gcgcccgcgt tccggccaag cttcgattgc gcaaaggccg gcaacgatgc
    12541 cgaacgcctg gtgtgcacgg acagtcagct cgcggcgctg gatgtgacga tggccgatct
    12601 ctatcagttg ggattgaaat cggtcacgga cacaaatgaa ttccgcgggg aacaggtggg
    12661 atggctaacc cagcgcgacg tctgcgccga caagcaatgc ctggtcgtct cttacaatga
    12721 ccgcatcaaa gacctccaac gctgggtacg gcattagcgc attcgccgcc gccccaggcc
    12781 ggttgggaaa tcttaagcga tcgcgaattt tccgtcttcg gtgagaacgt cccagaatga
    12841 cggccgctcc agcttcatcg ccgccaggac tttggaaagg ctacagaagc agggttgacg
    12901 cattccgtgg aaatcgacca gcggccccac gggctccatg tagaaggcca tcgcgtccag
    12961 catccgcttc agcgacggtt cgatcatgca ataccaatgg gttatgccgt ttttggcgct
    13021 catccggatc agcatctgaa tcaatcccag acgcatcagc ggtccggtgc ggtaatccgc
    13081 gggcgtcgca ttcgtcgtcg tggaaaggcg gcggatggac tgcttggaga tgctgaagcg
    13141 cgagacttcc gccgtcgaat gaaggggcac cgaggcagcg atcttcgtcg attcctcccg
    13201 caacatgcgc tggacggcga agctcttttc cggcgcgttc gacagcggca ggaccaggcg
    13261 cgcggtgccg agtgctattc ccgtgtcctt gtggagcaga agcccctgga ccgcatggga
    13321 atcgaattca tcgtgttcga gttcgtttgg gtgggcgttc gtgtcttcga actgcttctc
    13381 gacgcagtag atctggtatc gaagccggta ggctgcgccg acgaagggcg gcttgtccgc
    13441 atccacggtc atgaaatggg actggaaaca ttgaagaagc gattgttccg ctacgcttga
    13501 atttgaatag gtcgaaggtg catcgtctgt ggaattgaaa gcttcgtttt gaaacatgag
    13561 tcatttccct ccatcgagca catttggatc caagaggaca tgaccaccag aaacggtgcg
    13621 ctgcatcatt agatgtgatt tgctaaagat tgccgttcgc tcccgcagga tgatccgggc
    13681 acggagcatc ctcccaattt tttttatagt cgccgaaata ccgattgtgt tgtgataaaa
    13741 ttgagcgggg ggacaataaa cgaatcgttc atcattgtcc gcgcacgcag aacaattggt
    13801 gctcgtctta aacaatcttt aagggattac tttacggtgt taccgctgca ctgcagatcg
    13861 gcgcatccgc gttgcttccc gtcgcggcgt gcgtgcagat cttgagtcct ccatccgaac
    13921 ccaggagcca cagcaccgcg acattctcat tcacgccgga gccgacgcct tggtaggagg
    13981 cccgaacgat cggccccgcc gcgtgcgccg gaccggtgaa ttgttgcgag accgccgcac
    14041 cgattgccgc agtcgctacg cataatgcaa agattttttg acaccccatg acacgccttc
    14101 tccggtgaac tctgccggcg gcatcttgtc ggaatttgat accggcacga tagcgcaaat
    14161 gtttggcctg cgaatgattg gtctgttgcc gcgattttcc gaaggaccaa taaggccata
    14221 ccctacagac gtgtaggaca tcggctatgg ccgcgcctgc gcccgtgctt atttcttcac
    14281 gaaacggcgg aagttgagcg tgttggtgaa gcccccatag acattccccg catcgtccgc
    14341 ggcgacaccc tccagcgtgt tggtttcatt ccatggaatg aatgccgtca ccttgccgtc
    14401 cttgacgctg ccgatccgga tgccctgttt gtagcctggg ttgaccttgt cgctggacgt
    14461 cgaatccgcc acatagatga tgtcgttctt gttgatgtag acgccgctcg gccgaccgaa
    14521 ttgccgccac tcggcgagaa atttcccgtc ctgatcgaaa atctggatac ggctgttgga
    14581 acggtcggcg acaaaaaggc gccccgccga atccatcgcc aggccatggg gcgcgtcgaa
    14641 atcgccgggg cccgtaccgt gcttgcccca ggttttgatg aacttgccgg tcttgtcgaa
    14701 tttcacgatc cgcgcattgg tatcgccgcc atggccgtcg gcgacgaaaa tgtcgccgtc
    14761 cggcgcgacc aggatgtccg agggcgcatt gaaatgggtg gcgtcattgc ccgccacgcc
    14821 gggtgtgccc agcgtcatca gcaatttccc ctgcggcgag aatttcatca ccgtttggcc
    14881 cttgccgttg ttcttgacga cccggccgtc cgaaacccag atattgtcct gcgcatccac
    14941 atagatgccg tgcggataat tgaacaggcc tttcccgaaa ctgaacacga tcttgccgga
    15001 agcgtcgaat ttctggattg gatcgagatt ggaggccgag caatcgtccg ccgtctcgca
    15061 gcggtcgaac acccagatgc tcttgccgtc gcgatcggtg tccactgccg acaccgcccc
    15121 ccatttgcgc ccgcctgtca gctttgccca gccggaatcc tcgcgatagg tctgcggcgc
    15181 gctgttcgga tccggcgtga tttgcgcgaa accgctttgg acggaaaatg caatcgaggc
    15241 agcggcggca atgccaaacg cggatttggc ggcgtatgca tgacgcatga tgtttcctcc
    15301 cgtattttct tcccggatat tttcctggcc gttcacgccg cccaggcttt ttgcggcgga
    15361 aacgacgtgg ccgtgaaggc gatggccgtg atcgccttga cgctgcgcgc accatagcgg
    15421 aacggcatca ccaagcgaag gggcgcgcca ttctgcggcg gcaggggcgc gtcgttcgcc
    15481 agccaggcca gcatcacctg cggatgcatc agggttttca tgtcctcatc gaggtaataa
    15541 tcgtccgagg cggtgaagcg cgcataattg gcgaaactct gcgcgcccac ggacgccgcg
    15601 aaatcggaaa agcgcacgcc ggtccatttc acgatgccat ggggcttcac cgcgccgcat
    15661 tgcaaaagcg tgatctgcga atgacgcggc agcttctcca gatcgccgaa cgaaagcgtg
    15721 ccggacaatg tcgacgtgcc gcgtccatcg accttcactt tcatcttgcg ataatcgaac
    15781 tcgatgtcgg gcgtgtcctt ggtcttccac aggacgccat cgatggcgcc cgcttcggct
    15841 tgcgaatatt cgacggccga gccatccccc cgcaatggca gaggcgcgat cgtgcgcagc
    15901 cgcgtgtcca ccaaggcatc gggctgcgcc gcgtccgttt gggcgatagc ggagccgggg
    15961 cgcatcgccg ccacggaaag cacggcgatg gcggttgccg attggatcgt ctcacggcgc
    16021 gataggttca tggggcacct ttcgggttcg gttgcggtca ggccatggca ggcgcgtcaa
    16081 acctcatcgg ggtcttcaca ccccgcatcg ttttgcgcgc tcgaatgcta aacgctgccg
    16141 ttctcccgga acagatgaat tcgcgcgtgg ttcctacgca agaataacgc ttgtgaggtt
    16201 ccatcaaagc agcggcaatt gcggatcgga ttcgtccggc gacggccttg cccggcccgc
    16261 ctgcggcgcg ggccgcatcg tccagtcgcc ttcgcatggc accagcatgt ccttcagggc
    16321 ctggacgctc gccggttctt ctccaagcca tcggctccat cgatgcggct ggatcaccgc
    16381 gggcatgcgg tcggtgatcg ttgcgatcaa ccggttcggc gggacagtca ccatcgcgaa
    16441 tgtctcgagc cccttgcccg aacgaacctc gccccatcgc tcccagatga ccgcgatcgc
    16501 aatgggcgcg gcatcgttgg ccgcgataac gtgctgttcg gttttatggg gcgtgatctc
    16561 cttgccctcg ttgaacgtct gcacggccag gattccgcgc cgatgccgga acgcgtcgcg
    16621 gtaggttggc cgttcgtcga tggattcggc gcgtgcgtga atgaaggagg ccatgttcgg
    16681 gtctttcgcc cagggcggga cgagccccca acgcatgcgc gcggcctggc gttggccgtc
    16741 cgcagccagg tggatcagcg tggcgaagcg catgggtgtc acggtttcgg acgcgcttcc
    16801 cgcattgcca atgagatcgg cgagccggac atgggcgccc cagcccatca tttgcgtgaa
    16861 ttttccacac atcggatcgc ggcgctccag acgcaggaca aatcggtcac gcgaacgcgg
    16921 ctaaggaacc ttgccggggc ctgaccattc tattgtcgaa cggaaaacgg tgatggcaat
    16981 gaacaatatt atctatctgg tgggacttgt ggtggtggtc ggcgcgatcc tgtctttcgt
    17041 attccattga gcgggaaaat atcgctgtcg ccgcctcata tctgggcgcc tcaaatctgg
    17101 aggatctggt ggcccttggg cagcgagaaa ttttttaccg cgtgcaccag cccgtagatc
    17161 attgcttcct tcggcgtgat gtaaaggtcc ttgaagcggc gggcgaccaa aatctcctcc
    17221 ggaatcgcag cacgttcgcg caggatgctc tccgtgcggg tgtcgtccgc gagcgcggca
    17281 ttcagcgaac tttgcaagcg ttccgaggtc atgccctcct gcgaaggcat gacggtcgga
    17341 tggatcatga acatcgcgtg cggtgagcaa tagcgttcat gggcagccac aaaaacggtc
    17401 accgcgatgg aactgacact gccggtatta tggataacca cattgatcgg cagcgagcgg
    17461 atatgattgt aaagatagat gccatccgag acgtagccgc caagtgaact gaagcagaga
    17521 tgggcttcgt cggcgctgcc gttcaccgca acgttcaatg atgtggcgat gcgcgacacc
    17581 ccctcggaat cgatcggccc ggtgtatccc agataggcga tttttcgtcc cgccagatca
    17641 ggcatgcgag tacgtccatt gcatggcatt caagcgaacg ggattgctga aatccactcc
    17701 ggactccttg attgtcctgg ttttggcaat gttacgcggc tcagccgtaa accatacaag
    17761 tgcttgattt tgtcatatta ttgcgttcga caaagtgcgg cgcaacaagg aacgaatccg
    17821 gcagccgccg gtttttaacc atgggggcga gacttccaca aactctgagg aaatttccat
    17881 gggaaatgac aatatgaatc gcccgtccgg ccagggtcaa tcgggccagg gtcagtcagg
    17941 acaaggaaag caacagggcc agagcggcca gggacacggc agtcagggcc aacagggtca
    18001 gggcagccag ggccaacagg gccagggtag tcagggtcaa cagggccaga ccagcaaaca
    18061 gggtcagcgt tcaggccgtt cgagttccaa cgaatagtcg tgaatcacgg gcaatagccc
    18121 ggaacacaga agacaactga agctcggctg tggggctaac gcagccgggc ttctttattt
    18181 cgcgccagcc ggagcgtcgc cattccaacc tcgcgattta gatctacatt gtatctatcg
    18241 ggccgggcgg cgcaggaggt gcgctttgga tttcgcactg ggtgaaaccg cggatctgct
    18301 gcggcaaacg gtgcggggct tcgcagccga tcatatcgcg ccgcgtgccg aggctatcga
    18361 ccgcgaaaat acattcccgc gcgatctgtg gcccaagctc ggcggcctag gcctcttcgg
    18421 cgtgaccgtg gccgaggact atggcggcgc cggcatgggc tatgtcgagc aggcgatcgc
    18481 ggtggaagag atcagccgcg cttcggcctc ggtcggcttg agctatggcg tgcatgcgac
    18541 gctctgcatc aatcatctgt accgcttcgg caccgaggag cagaagcgcc gctatctccc
    18601 gcaactgctg tcgggcgaat gggtgggggc cctggccatg agcgagcccg gcgcgggttc
    18661 ggatgtgctc gccatgacca cccgcgcgga attgcagaac gggcgctatg tcctgaacgg
    18721 caccaagatg tggatcacca atggaccggt ggccgatttt ctcatcgtct acgccaagac
    18781 cggcgatccg tccgggcggc gcggcatcac cgcctttttg atcgagaaga gcttcaaggg
    18841 attctccacc gcgcagaagc tcgacaaatt gggcatgcgc gggtccgata cgggcgagct
    18901 gctcttcaag gattgcgaag tgcccggcgc caatgttctc ggcggtgtgg acaaggggct
    18961 ctccgtgctg atgtccggtc tcgattatga gcgggtggtg ctctccgcgg ggccggtcgg
    19021 catcatgcag gcgtgcctcg atctggtgct gccttatgtg cgccagcgca agcagttcgg
    19081 ccgggccgtc ggcgaattcg agctgatgca gggcaagctt gccgacatgg atacgacgct
    19141 gaacgcggcg cgcgcctatg tctacgcggt ggcgcgggcc tgcgattccg gaacggtcac
    19201 gcgcaaggat gcggcgggcg tcatcctttt caccgcggag cacgcgacgc agatggcgct
    19261 gcaggcgatc caggcgctcg gcggcatggg ctatgtcaac gagatgcccg cggggcgtct
    19321 gctccgcgac gccaagctct acgagatcgg cgccgggaca tcggaaatcc ggcgcatgct
    19381 gatcgggcgc gaattgttcg aaggacggga ataatgtccg ctgagacgat tgtcatcgcc
    19441 ggtgccgcgc gcacgcccat cggttcgctg caaggccaat tcaagagctt gtccgcgacc
    19501 gcgctcggcg cggttgcgat tggcgcggcg ctggaacgca gcggcctgga ttcggatgcc
    19561 cctggcgaag tcttcatggg ctgtgtgctg tccgcgggcc tcggtcaggc gccggcgcgg
    19621 caggccgcga tcgccgcagg cattccatac ggcgtggggt gcaccaccgt gaacaaggtc
    19681 tgcggctccg gcatgaaggc cgccatgctc gcccatgacg ccattctcac cggcagcgcg
    19741 aatgtggtcg tcgcgggcgg catggagagc atgaccaatg cgccctatct tctgaagaaa
    19801 gcgcgtggcg gttaccgcct gggtcacgat gcggtctatg accacatgtt cctcgacggg
    19861 ctcgaagacg cctatcggcc gggcacgctg atgggacaat tcgccgaaga caccgccgag
    19921 aaatatcaat tcacccgcgc ggtccaggat gaattcgcaa cgctgtctct ccagcgcgcg
    19981 aaacatgcgg ccgccgaagg aattttcgcc cgcgagatcg ccggagtgcg cgtgggcgat
    20041 gcggagatta ccgaagacga gcttcccgcc cgcgccgatg ccgcgaagat tccgcgtctg
    20101 aaaccggctt tccgcgaagg cggcacggtg accgcggcca attccagcgg catatcggac
    20161 ggcgcggcgg cgctggtgct gatgagccag gacgaagccg accggcgcaa tgtgcgaccg
    20221 ttggcgcgca tcgccggtca cgcgagccac gcgcaggcgc cggctctctt caccaccgcg
    20281 ccggtccgcg ccatccagaa attgcgcgag aagatcggct ggaatcccga cgatgtcgac
    20341 ctttatgaaa tcaacgaggc ctttgcggtg gtggcgctgg ccgcgatgcg cgatctcgac
    20401 attccccgcg acaagataaa cgtgcatggc ggagcatgcg cgctcggcca tcccatcggc
    20461 gcgtcgggcg cgcgcatcgt cgtgaccttg ctgaacgcgc tgcaggaaaa gaatttgaag
    20521 cgcggcatcg caagcctctg cattggcggc ggcgaggcca ccgcgatggc cgtggaactg
    20581 atctgacatg gccgttctca ccacgcgcct cgaccgtgct tccaaggaat acgccgccaa
    20641 tgcgcgcgcg atggaggcgc tggtggacga tctccaggcg cggctcgcga ggattcgtct
    20701 gggcggcggc gaagccgcgc gcaaccggca caccgaacgc ggcaagctgc tggcgcgcgc
    20761 gcgcatcgcg gcgcttctcg acaaaggcgc ggatttgctg gagctgtcgg cgtttgccgc
    20821 tgatggcgtg tacgacgatc ccctgcccgc cgccgggctg atcaccggca tcggcacgat
    20881 tgcgggccgc gattgcgtcg tcgtggtcaa cgacgccacg gtgaagggcg gcacttatta
    20941 tccgctgacc gtgaccaaac atctgcgcgc gcaggacatc gcgatcgaga atcgtctgcc
    21001 ctgtctgtat ctggtggatt cgggcggcgc gttcctgccg ctgcaggatc aggtctttcc
    21061 cggccgcgat catttcggac gcatcttcta caatcaggcc cagctctcgg cggcaggcat
    21121 tgcacaaatc gccgccgtac tcggttcctg caccgcgggc ggcgcctata tcccggccat
    21181 gtgcgaagaa agcatcatcg tcaaaggcca gggcacgatt ttcctcgccg ggccgccgct
    21241 ggtgaaagcc gcgaccggcg agaccatcag cgccgaggaa ttgggcggcg ccgagacgca
    21301 cgcgcgactc tccggcgtgg tggaccatgt ggcgaaggac gatgcggacg cgatgcgcca
    21361 gatgcgcggc atcatccgca acctgcccgc gccgcctgca accatagcgc ccaaagaggt
    21421 gcacccgccg ctttacgatg ccgaggaact gggcggcatc gtgccggcag atttgcgctg
    21481 gatctacgac gtgcgcgaag tgatcgcgcg catcgtggat ggcagcgaat tcgacgaatt
    21541 caagccgctt tacggcgcca cgctggtttg cggctttgcc catgtccacg gccatatcgc
    21601 gggcatcgtg gccaacaatg gcatcctgct gtcggaggcg gcgctgaagg cgacgcattt
    21661 catcgagttg tgctgccagc gcggcgtgcc gctgatcttc ctgcagaaca ccagcggctt
    21721 catggtcggg cgcaaagccg aggccgaggg catcgccaag cacggcgcca agatggtgat
    21781 ggcggttgcc aatgcccagg tgccgaagat caccatcatc atcggcggca gtttcggcgc
    21841 gggcaattat gccatgtgcg gacgcgcctt ctctccgcgc ttccttttcc tatggccgaa
    21901 tgcccgcatt tcggtgatgg gcggcgagca ggccgcacag gtgctttcgc gcatcaagcg
    21961 cgagcctggg caaacccagc ccgacgcagc gcagatcgcg cgcgccgatg aaatccgcaa
    22021 tcaatatgca acgcagggcg cggcctatta tgccagcgcc cggctgtggg atgacggcat
    22081 catcgcgccg gccgagacgc gcgacgtgct tggccgctgt ctgggcgttg tgggcaatgc
    22141 gcccaaggtt ccaaccaaat tcggcgtctt ccggatgtga cgatgcgcgt tcccaaatcc
    22201 gtccgcatgg tggaagtggg tccgcgcgac ggcctgcaga acgaatccgc ggcactttcc
    22261 accgccctga aggtggaact gatcgaccgc ctgagcgcgg ccgggctgaa gaccatcgaa
    22321 gccggaagct tcgtctcgtc caaatgggtg ccgcagatgg cggacacggc aatggtcctg
    22381 gcgcgtatcg aacgtgcgcc cggcgcgagc tatcccgtcc tggtgcccaa tatgaagggc
    22441 ttcgaggccg ctcgcgcggc gggcgcggac gagatcgcca tcttcaccgc cgcgtccgaa
    22501 agtttcgtgc gggcaaacat caattgttcc attggggaga gcttcgaacg ctttgagccc
    22561 gtctgcgccg ccgcttgcgc atcgggcatg aagattcgcg gctatgtttc ctgcgcgctg
    22621 ggttgccctt atgaaggcga aatcgcaccg gcgcgcgtgg ccgcggtgac aaagcggctg
    22681 ttgcagttcg gctgctatga agtctcactc ggcgacacga tcggagtggg gaccccgcgc
    22741 aagacgcaaa tcctgctgga gaccgtgagc gcggaatgtc cccgcgagaa actcgccgtg
    22801 cacttccacg atacctacgg ccaggggctc gccaatatcc tggccgctct ggaaatggga
    22861 atcgcggtgg tggattcgtc ggtggcggga ttgggcggct gtccctacgc gcccggcgca
    22921 tcgggcaatg tggcgagcga ggacgtactc tacatgctga gcggcatggg tatccaaacc
    22981 ggcgtcgacc tcggcctcct ggtggaaacc ggacgtttca tcgccggcgc attccacaga
    23041 ccgcccggct ccaaagccaa catcgcgctg tcgaaccgga cttccccttc aggctgaatg
    23101 gagttttcga ggattcatgg aaccgcaagc gtggcaaatg ggttttgatg ccgggccggg
    23161 attttgagag tgaacaaaca cggcatgccc ggcatcgaac gcatcgtcgc agaatttcaa
    23221 atccggcatc ggcaatgtct ggatgcaacc ggaaccctca tcggtgaacc gcccgcattc
    23281 gcgcacgatc gcgacatcat gatcgcgctc tatcgcgcca tgacgcttac ccgcatcttc
    23341 gatgccaagg ccatcgcgct gcagcgcacc ggccagctcg gcacttatcc ttcctgcgcc
    23401 ggtcaggaag ccaccgggat cggctacgcg agcgccatgg cggcggacga cgtgatgttc
    23461 atcacctatc gcgaacaggc cgcgcagatt tggcgcggcg tcacgctgac ggaactcctg
    23521 caattctggg gcggcgatga acgcggctgc aactatgcgg gcccgcgcga ggattttccg
    23581 cccgccgtgc ccatcgcgag ccaggcgacc cacgcggccg gcgcagccac cgccttcaag
    23641 ctgcgccatg agaaacgcgt ggctgtctgt gcgctcggcg atggcgccac gtccaagggc
    23701 gatttctacg aatcgatcaa tctcgcgggc gtctggaggc tgccggccgt cttcctggtg
    23761 atcgacaatt tctgggcgat ctcgctaccg cgcgagaaac agaccgcaac cggaacgctc
    23821 gcgcaaaaag cgattgccgg cggcatcccg gggatacagg tcgatggcaa cgacgtgatc
    23881 gcggtgcgcg ccgcgatgga cgaagccttg gcgcgggcgc gggccggcga aggcgccagt
    23941 gtcatcgaag ccatcaccta ccgcatgggc gaccacacca ccgccgacga cgcgtcccgc
    24001 tatcgccgcg aggaattggt gagcgcggcg tggaagctcg atcccttggc gcggctgcgc
    24061 acctatatcg cgaaccggca ttggtggacc aaggccgacg aggaacgcct gatcggcgaa
    24121 tcccgcgaaa aagtggacgc ggcggtcgcc gattatctcg cgattgcgcc gcccgcgccg
    24181 gaatcgatgt tcgaccattt gttcgaaacc ctgcctgcct cgcttcaatg gcagcgcgac
    24241 cgcatggcaa ggcgctgata tggccgagat caccatggtg ggcgcaatcg ccatggcgct
    24301 ggcgcgcagc ctcgccggcg attcgcgcgt gctggtgctg ggagaggatg tcggcgccaa
    24361 tgggggcgtc tttcgcgcca ccgatgggct gcagcagcgc ttcggcgaag aacgcgtcat
    24421 ggacacaccg ctcgccgaaa ccgttctcgc cggcatgtcg atcggtttgg cggcgcaggg
    24481 ttttcgcccg gtgaccgaat ttcagttcat gggattcatc tatccggcgc tcgacgaggt
    24541 catcaatcat gcggggcgcc tgcgcaaccg cacgcgcggg cgtctttcct gcccgcttgt
    24601 cttccgcgcg ccgatgggtg cgggaatcca cgcgcccgag catcattcgg aaagcaccga
    24661 agccctgttc gcgcatattc ccggcatccg cgtggtgatc ccctcgtcgc ctgcacgcgc
    24721 ctacggcctg ttgttgtccg ccatcgcgga cccggacccg gtgatgttct tcgagcccac
    24781 gcggctctat cgcgcggcga aagaagaggt gggcgacaat ggccagggtc tgccgctcga
    24841 tgtgtgtttc ctcttgcgtg aaggcgccga tgtgacgctc gtcacctggg gcgcggcggt
    24901 gaaggaaacg cttgccgccg ccgaattgct cggcgccgag ggtatctcgg cggaggtcgt
    24961 cgatgtggcg accgtcagcc cgctcgactt cgagacgatc ctggcgtcgg tcgaaaagac
    25021 cggccggctg gtgatcgtgc atgaagcccc gcgcaattgc ggcatcggcg cggagatcgc
    25081 cgcgcgcatg gccgagcgcg ggcttctgtc gctgcaggcc cccatcgagc gcgtgacggc
    25141 gcccgacgtg gtggtgccgc ttctcagatt ggagaaagcc tatatgccgt ccgaacagga
    25201 catcgtggag gcggcgcgcc gcgccgtgag cttccaatga ggacttttct tcttcccgat
    25261 ctcggtgaag ggctgagcga agcggagatc gtttcctggc atgtgtcgcc gggcgatcat
    25321 gtggtgaccg accagccgct ggtctcggtc gagaccgaca aggcggtggt cgaagtgccc
    25381 tcgccctatt cgggcacggt cgcggcgctg catgccgcgg ccggagatat cgtgcgcatc
    25441 ggcgcgaagc ttgccgattt cgacgaggcg ggcgcccatg aagacagagg cacggtggtg
    25501 ggcgccgtcc cgcccgccga atcgtccacc accggacaga ccgccgaacg cactcccgtg
    25561 ccgggcgcga cattgcgcgc catgccggtg gtgcgcgcgc ttgcccgcaa gctcggtgtc
    25621 gatctcgcgg gggtcgaacc gagcgggccc aatggcgcag tcaccgccga agatgtgacg
    25681 cgttacgcct cggccatgtc ggacgccggt ccgggcgagc cgctgcacgg cgtgcgccgc
    25741 gccatggcgc agaaaatgga gcaatcccac gccgagatcg ttccggcctc tgtctatgac
    25801 gaagccgacg tcgaggattg ggtggagggc ggcgacgtca cggtcgcgct gatccgcgcc
    25861 ctggtggcgg gctgccgcgt ctcgcccatc ctgaacgcct ggtacaatgg cgcggacaag
    25921 atggtgcgca tcgtcggaaa gattcacgtc ggcatggcga tcaacaccgc cgacggcctg
    25981 ttcgtcgcgg tgatgcgcga tgtggcgggg cgcgacgaca ccgatctgcg ccaggggctg
    26041 aacaatctta aacgcgcggt ccaagcgcgc cggattccgc tcgaggaatt gcgcggcgcg
    26101 accatcacgc tttccaattt cggcgtctcc ggtgcgggac gcttcgcgaa cctcatcgtc
    26161 gtaccgccac aagtggccat tgtcggcgcg ggcacgatca agccgcgcat tgtcgcgcgc
    26221 gccggggccc ccgcggtgcg ccgcaccctg ccgctgtcgc tgaccttcga tcatcgcgtg
    26281 gtcacgggga tcgaggccgc gaacttcctg aagtccatgg tgacggccct ggaaaccaga
    26341 ccggtcacgg tcttaacccg gcagtaaaaa caatcgcgct aagacaggct gtctcttttt
    26401 agaagacagc gtcaggcgat gaacgacaaa tcccctcttc cgttcaccaa gcgcgagcgg
    26461 cgcagcgcgg accggcgtca aaaacgccgc ccggcggtcg agttacgcgg tgaaatccgc
    26521 atgggacgca aatcctggcc cgtgctcatc ggtgaaatac ccggttcggg cgcgctcgtg
    26581 ttcaccgatt cgccgccgcc cgtcggcaac gccgtcgagc tatggatcga gggcttcggc
    26641 gcggtgccgg tcgaggtcgt gcattcgagc agacgtctct gcggtctggt gctgtgcgat
    26701 ccggccgcct atcgcgccga actgctgctg tggctgcgac ggatgctgaa gaccaaaaca
    26761 agcgcgcagc cgaaggaccg gcgcacgccg ctgttctttt gcgcgcgcgt cgcggagctg
    26821 gcgtcctacc gctccgcact cagcccttcc catcaaaccc gctagcgttt ttgcagccgc
    26881 gccacgccgg cctgcgcgag gccggtgatt tcgttcgcgt ccggcttcgg ccccaccaga
    26941 tccttgtaga gaccgagggc ttcatccttt ttgccttggg cctcggtgaa atgcgccgtg
    27001 agataggcca cgcctcgttt gtaattgtcc gaaatatcac cctgcttgag ccaatccaga
    27061 aagacgatct gcttgtccgc cggcagggtc atggaaatcc gtgccatcag ctccgtgtcg
    27121 tgcagagaga cggaataata ccaaagcaga gtgccctttg tgtggtcgtc gatattgcgg
    27181 cccgcaacgc ggattttatc ggcatattcg attgcgccgg cgatgaaagg cgaagacgta
    27241 ttgttggtga gccccgccat gatcatcgag tcggagaacg caggatcgct gaagctggcg
    27301 agcgccacgt cgagatcggg gcgcgctttg tcgaccggtc cccctttcca gagaatccag
    27361 aaaccccgca tgacgtggcc gtacatattc gccgaatcgt ccgcgatggc cgcgttgaat
    27421 tcttcggtga tgtcggtttc cggattgccg tcgcgcgaac gcaggaaatt cgcccagccg
    27481 atatgggcct cgagatcggc cttttccgcg tcctcggtgc cgggcagatg ttcgatcagc
    27541 gccgccttca gcgggtcgac gatggagctg aacgtccttg gcccgttcgc ggaccccgta
    27601 tgcacgtcct cgagccatct catcgcgacg cgcgcctgct gcgccgacgc ctccgcgttt
    27661 cggggcgcga tctgccgggc tttggcgttg gcggtccatg ccgcctcgta gtccttgtgc
    27721 gctaggcgct cgtcggccgt ttgcgtcagg gtgcggacct ccgactggcc ctgaaaatat
    27781 tccgtcacat gcgagaagaa attcgcacca ttgttgatgt agccgattgt cgccgaaacg
    27841 acgccgagga ccgcgacaac cgcggtgatg gatttcgccc agcggcgcag gcgcgaaccg
    27901 ccggtgcctg tggccggcac atcccgattt ggcgtctgag cgtcagtccc catgcggcat
    27961 cctcgtgggt ggtgtctcag ttttgaattt ctacgatccg cgcaacgtgg cgagcagaat
    28021 gaatatgaga ccaatgaata gcggtgctct atacacaatt cggttctttc ccaggcggcc
    28081 tggccagaaa atgcgcgccg cggcgatcat gcccccaaga atcagcaacg cctctccggc
    28141 caaacgaagc atcgtcgcgt tttccactcg ggctctccct ctcctcggac gggtgccata
    28201 gtatcgtcat gcccaaagga gcccaagacc ggaaacgatc cccggatgtt ggcggcaacc
    28261 cgtttatagc tgtgcgaccc gttcagggtt cgcgcagcgc cccgcccggg ggtggaacga
    28321 tcatcaggtc ccggggaacg ccaaggcccg cgtaatagcg gcgcagcaga acgagcgaca
    28381 ggacggtgcc ggccagcgac agcagaattc ccaccggcaa gggcgcgccc gcccacgaca
    28441 aggccgccac cgccagacac cccgccgcgc agccgatgtc ccagccgcct tcggtcgcga
    28501 cgtgaaagcg cagcgcgcag ggcgagcgct tggcctgatt gtagaccgcc gtcatcagcg
    28561 tcgggatgta gaggcacccg gccagcgtcc ccagcgcgtt c
//
LOCUS       DASYEG010000132        14142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_40028, whole genome shotgun sequence.
ACCESSION   DASYEG010000132 DASYEG010000000
VERSION     DASYEG010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 14142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14142
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40028"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1914
                     /locus_tag="VGM72_05695"
     CDS             <1..1914
                     /locus_tag="VGM72_05695"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY4264791.1"
                     /translation="EGSLINYAYRVAFARDATPELFHANLEAAFPKASWEFNDRSNAL
                     PQVTRFVEQATMFLTLVGLTALIVAGVGAGQAVEAFLERRRATIATLKALGAEGDLIF
                     FIYLLQIMTVAALGLALGLALGAAMPFAVEYFAGASIPAPAHYGVYPGPLALAAVFGT
                     LAALGFAIPPLARAREIAPAGLFRDLVAPSARGGRWPYRAAAGAIFAIIAALAVALSP
                     YPRFSLGFLGGTIAVLAVLRLMAAGVRLGLVQLPRPKSQTLRLALTNLTRPGTPTASI
                     IVALGLGLTLLATIVLMQASVEAQIEDQLPSRAPSFFFIDIQQNQIDAFRTLVSAIPG
                     SADFNATPMLRGRIVKLNGVPVEQVNIAADSRWAVNSDRAVTYTAAKPKDARVVEGPE
                     WWPAGYHGRPLVSFDRDLARGMGLKIGDTITINLIGRDIDLTIFNLRDIDFRTGGINF
                     VLTVSPGIVSQAPHTFLATLRTAPAQEDATFAAVSKAFPNVTVVRVKEALAQVGELLA
                     ALARGIEIASLVTILAGVLVLAGAIAAGHRARLYDAVVLKVLGATRARLGAVYAVEYG
                     VLGALAGCAALLAGTLAAWAVAKFVLDIPFVFATTALLFTIAGGALGTLILGLSGGFA
                     ALAAKPAARLRNP"
     gene            2018..2755
                     /locus_tag="VGM72_05700"
     CDS             2018..2755
                     /locus_tag="VGM72_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010657904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEY4264792.1"
                     /translation="MAEYDNRVRSWGGADATAIDVGLRAYMLRVYNYMLMGLGLTGLT
                     AWFTLSTPAFANLFFTVTARGYTLAPLGWVVLLAPLGLVFFLSWRIQQMSFAAAQMTF
                     WVYAGLMGIGLTPVLYLYTGASVEQVFFVTAATFGAMSLWGYTTKRDLSGLGSFLFMG
                     LIGLIIASLVNMFMQSSMMQWVISVMGVLIFTGLTAYDTQSIKETYYVGDDGAVAGKK
                     AVMGALRLYLDFINLFIMLLRLMGDRR"
     gene            complement(2841..3017)
                     /locus_tag="VGM72_05705"
     CDS             complement(2841..3017)
                     /locus_tag="VGM72_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004153593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEY4264793.1"
                     /translation="MLHYALVFLVLGLIAALFGFTGIAGASIGIAKILFFVFLALFAI
                     ALLMGGMRGRGTMT"
     gene            complement(3205..3636)
                     /locus_tag="VGM72_05710"
     CDS             complement(3205..3636)
                     /locus_tag="VGM72_05710"
                     /inference="COORDINATES: protein motif:HMM:NF022437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2794 domain-containing protein"
                     /protein_id="HEY4264794.1"
                     /translation="MLRKMVDEPIVFGRAKGPHFARTSSRGTVSDNGPASGPETRDTR
                     IRFDRSELKRILNTYGRMVMAGEWRDYAIDFREDVAVFSVFRRSSEMPLYSIEKRPKL
                     KERQGQYCVVAAGGQVLKRGPDLEQVLRVLDRKLLRAVAAE"
     gene            4122..5093
                     /locus_tag="VGM72_05715"
     CDS             4122..5093
                     /locus_tag="VGM72_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4264795.1"
                     /translation="MIILNGLTKTFGSIAAVSGIDLSVRKGEVLGFLGPNGAGKSTTM
                     KMIAGYFTPTSGRVSICGHDVENDTIAAQAQLGYLPEGAPAYGDMTPSQFLTFIAHAR
                     GFWGAEAKQRVNKATLLTELESVMEQPIETLSKGFKRRVGLAQAILHDPPVLVMDEPT
                     DGLDPNQKHSVRTLIRGMAADKAIIISTHLLEEVDAVCTRAVIIDRGRIVADGTPASL
                     LARSRHHNAVTLTLHKDQAAAAKVALQGLPGVASAETVSEADGLATITAYPRENELII
                     ENVSTLVAAQKWDVKELYAEAGRLDDVFRALTTHDTQRVRERNGAAA"
     gene            5090..5824
                     /locus_tag="VGM72_05720"
     CDS             5090..5824
                     /locus_tag="VGM72_05720"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4264796.1"
                     /translation="MRPVLAIFKREFAAYFATPLAYVFIVIFLLAMGAFTFYVGHFYD
                     NGVANLSVFFGYHPWLYLFLVPAVGMRLWAEERRLGTMELLLTLPIPIWASVLGKFLA
                     AWAFVGVALALTFPIWITVNYLGNPDNGVIVASYVGSFLMAGGYLAISAAISATTNNQ
                     VIAFVVSVVVCFLFTISGAPLVLDVFQAWAPIVLVNTISSFSFLTHFQAISSGVIDLR
                     DLIYFGSLIALFLSVNVVLVDLKKTN"
     gene            5826..7772
                     /locus_tag="VGM72_05725"
     CDS             5826..7772
                     /locus_tag="VGM72_05725"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HEY4264797.1"
                     /translation="MKPLSHRVFALSAIALAVVLFVAINIVANQWLGTARLDLTQNGL
                     YTVSPGTEAIVSKLPEPVTLRFYFSRQSSAGYAQIVAYAGRVHDLLQEYAALSHGKII
                     LEELDPTPFTQAEDDAVAQGLTGAPTQEGDNVYFGLVGTNTLAGHEVIPFFDQSREEY
                     LEYDLTSMIYKLSQPAKPKLGILSALPLEQGAGGLQAALAGNAQPYAIYQQLRDNYDV
                     QMLDPMTTDRIPADVGTLLVVHPANFTSSVQYAIDQFVLKGGHAIVFVDPLSESSVQQ
                     GPGAQDQGDKSSSTLGPVFTAWGLDYDPTKVVADADLAQAVQVAGPNGQPRVIDYIAW
                     LRMMPANFDRNDPITSNLQVLNIASAGSLKPHQGATTKFQPLLTSSTTAALLDSILVR
                     TVQNPSDLLRRFSPTGARFTIGARITGPAKTAFPNGAPAAPPKPPADSNEPPKPDAGA
                     LPAQIKEAKNINVVVIADSDLLDDRFWVQVQNVVGQRIAVPTADNGALVMNAVENMMG
                     SNDLISLRTRERSARPFVVVEQIRRNAETRFLAEEQQLQQKVTATEASLRALQGQNQP
                     QGGAAPVLSKEQQAQIDKFRRDLVTTRTSLRGVQASLRQDVERLGTMLAFLNIALVPI
                     GIGAAAIGLSVLRQRRRRKAKALT"
     gene            7769..8875
                     /locus_tag="VGM72_05730"
     CDS             7769..8875
                     /locus_tag="VGM72_05730"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEY4264798.1"
                     /translation="MSDMAMSARQDPRRRSLMILGGVTALFVVLAVAAVLQQASLVAP
                     KFAERPFFPGLNDSINNVGEIAIASKTGTFHVRLNQGKWSVVEKGGFPADAPQARSVA
                     AGMAGLTALEPKTNRADWLKYVDLDAPDKGGNAIDVKLTDNMGKPMAEILAGKNQGAA
                     DDLGRTQLYVRKPDETQAWLVRGNLTPKPNAADWLDKNVINIARDRVKGATVTPPMGP
                     AYTLSRDSKDQQDFKLLDMPAGRQLSFEGSPDGVGSALIGFDFDDVAKADSIDFSKAP
                     QSVTHTFDGLDITVKIATKGMDRWASIAAAGTNPMTQNEAMGIDARLSGWAFKLQPSK
                     ADQFVATRETLLKPPGGPATPAAAPGRAPAFPSR"
     gene            8949..9989
                     /locus_tag="VGM72_05735"
     CDS             8949..9989
                     /locus_tag="VGM72_05735"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY4264799.1"
                     /translation="MAEEPLYLRNQWYMAALSSSLKTGALRREMLLAEPVLLGRGRDG
                     LVFALRDICPHRGVPLSAGKILPENTVQCPYHGWRFKPDGVCSLIPSTVEGQDGIDPS
                     KVRVRSYPLREQDGLIWIYMAANERDAASPSEPPRVPLANATPRWVESQMFPCGIDHA
                     VLGLMDPAHGPYVHAHWWWHKAPREKVKHYAPLPNGFVMTRHKPTKPVYSMIGDVSTE
                     ITFELPSTRFENISGKLFGRPIDVIGLTVCTPRDAETTQVTQIFYWPAWLGFIRPFFM
                     ALGPTFLGDDRKIVALQREGLKFNPPLMLIQDSDVPAIWYHRLKKAWAESVETGKPFE
                     NPVQERTLRWRS"
     gene            10110..13379
                     /gene="carB"
                     /locus_tag="VGM72_05740"
     CDS             10110..13379
                     /gene="carB"
                     /locus_tag="VGM72_05740"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY4264800.1"
                     /translation="MPKRTDIHTIMIIGAGPIVIGQACEFDYSGAQACKALRAEGYRI
                     VLVNSNPATIMTDPGLADATYIEPITPEFVEKIIVREKANVPAGRIFALLPTMGGQTA
                     LNTALDLYESGVLASHGIEMIGAKAGAIAKAEDRQLFRQAMESIGLESPRSVFVMDQK
                     EALAALERIGLPAIIRPSFTLGGTGGGIAYNREEFAELAAGALEASRGHGILIEESVL
                     GWKEYEMEVVRDRADNAIIICSIENIDPMGVHTGDSITVAPALTLTDKEYQRMRTASI
                     AVLREVGVETGGSNVQFALNPADGRMVIIEMNPRVSRSSALASKATGFPIAKVAAKLA
                     VGYTLDELTNDITQVTPAAFEPTIDYVVTKIPRFAFEKFPGADPILTTSMKSVGEAMA
                     IGRTFAESLQKALRSMETGLTGLDEIAIPNLGQGDDANAIRAALGRPTPDRLRVAAQA
                     LRLGIREEELTRITAYDPWFIGEIAQILRTEAAIRASGLPKDADGLRRLKAMGFSDAR
                     LATLTGLKESDVAMARAHLGVHPVYKRIDTCAAEFAALTSYMYATYETPVDGEAECEA
                     RPSERRKVIILGGGPNRIGQGIEFDYCCCHACFALSAEGYETIMVNCNPETVSTDYDT
                     SDRLYFEPLTAEDVLEIIRLEQTKGTLAGVIVQFGGQTPLKLAGALGQAGIPILGTSA
                     DAIDLAEDRQRFLELLKTLGLKQPQNGTALDIDTAVNVARGIGYPTILRPSYVLGGRG
                     MIIVADEPALKAALQSGELFRISGANPVLIDGFLQHATEVDVDAIADETGDVFIAGIM
                     EHIEEAGIHSGDSACALPPHSLDAATIAELERQTVALARALQVRGLMNVQYAIQNGEI
                     FVLEVNPRASRTVPFVAKAIGLPVAGIAARVMAGEHLAGMALARAKKSHIAVKEAVLP
                     FARFPGVDTVLGPEMKSTGEVMGLDTDFGRAFAKSQIGSGAKLPLSGTVFISVRDADK
                     AGASGPAAQLIAMGFDIVATRGTAKYLQASGLPVRIVNKVLEGRPHIVDAMKNGSISL
                     VFNTTDGTQALSDSASIRKTALQMKIPYCTTMAGAAAVTQAIHSLKSGSLEVAPLQSY
                     A"
     gene            13520..13993
                     /gene="greA"
                     /locus_tag="VGM72_05745"
     CDS             13520..13993
                     /gene="greA"
                     /locus_tag="VGM72_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY4264801.1"
                     /translation="MERIPMTAAGYKALEDEIQHLKLEERPAVIKMITEARAHGDLSE
                     NAEYHAAKERQAFIEGRVMDLEDKLSRADVIDVSKLSGKTVKFGATVTLADEDNDSKV
                     KYQIVGDLEADAKHGRISISSPIARALIGKAAGDTVEVAAPGGARSYEILKVQFA"
BASE COUNT         2596 a         4554 c         4302 g         2690 t
ORIGIN      
        1 gaaggcagcc tcatcaatta tgcctaccgc gtggcattcg cgcgcgatgc cacgcccgaa
       61 ctgttccacg cgaatctgga agccgccttt cccaaagcct cctgggagtt caacgatcgc
      121 agcaacgcgc tgccgcaggt gactcgcttc gtcgagcagg cgaccatgtt cctgacgctc
      181 gtcggcttga cggcgctcat cgtcgcgggc gtcggcgccg gtcaggcggt cgaggcgttc
      241 ctcgaaagac ggcgcgccac catcgcgacg ctgaaagcat tgggcgccga gggcgatctg
      301 atatttttca tctatcttct gcagatcatg acagtggccg cgctggggct ggcgctggga
      361 ctggcattgg gtgcggccat gccattcgcc gtcgaatatt tcgccggcgc gtcgatcccg
      421 gcgcccgcgc attacggtgt ctatccgggg ccgctcgcgc tcgcggcggt cttcggcacg
      481 ctggccgcgc tcggctttgc cattcccccg ctcgcccggg cgcgggagat cgcgcccgcg
      541 gggctgttcc gcgatctggt cgcgccatcg gcgcggggcg ggcgctggcc ctatcgcgca
      601 gcggccggtg cgatcttcgc gatcatcgcg gcgctggcag tcgccttgtc gccctatccc
      661 cgattcagtc tgggcttcct gggcggcact atcgccgtgc tggccgtttt gcggcttatg
      721 gcggcgggag tacggctcgg tctcgtgcaa ctgccgcgcc cgaaatcgca aaccctgcgg
      781 ttggcattga ccaatctcac gcgtcccggc acgccgaccg ccagcatcat tgtggcgctt
      841 ggcctcggtc tgacgttgct ggcgaccatc gttctgatgc aggccagcgt cgaagcccag
      901 atcgaggatc agcttccgtc gcgcgcgccg agcttcttct tcatcgatat ccagcagaat
      961 cagatcgatg ctttccgcac cctcgtctcc gcgattcccg gctcggcgga tttcaacgcg
     1021 accccgatgc ttcgcggacg aatcgtgaaa ctgaacggcg tgcccgtgga gcaggtcaat
     1081 atcgcggccg attcgcgctg ggcggtgaac agcgaccgcg ccgtgaccta tacggcggcc
     1141 aagcccaagg atgcacgggt ggtcgaaggc cccgagtggt ggcccgccgg ttatcacggc
     1201 cggccgcttg tttccttcga ccgcgatctg gcgcgcggca tgggtctgaa aatcggcgac
     1261 accatcacga tcaacctcat cggccgcgat atcgatctca ccatcttcaa cctgcgcgac
     1321 attgatttcc gcaccggggg aatcaatttc gtcctcaccg tgtcgccggg aatcgtctcg
     1381 caggcgcctc atacgttcct cgccacgctg cgcaccgctc cggcgcagga agacgccacc
     1441 ttcgcggccg tctcgaaggc atttcccaac gtgaccgtcg tgcgcgtgaa ggaagcgctg
     1501 gcgcaggtcg gcgaattgct tgccgcactt gcgcgcggca tcgaaatcgc cagcctcgtc
     1561 accatcctgg ccggcgtgct ggtgcttgcg ggcgccatcg cggcgggaca tcgcgcacgg
     1621 ctctacgatg cggtcgtgtt gaaagtgcta ggcgccacgc gcgcacgtct gggcgcggtc
     1681 tatgccgtcg aatatggcgt gctcggtgcg ctcgcgggat gtgcggccct tctcgccggg
     1741 acgctggcgg cttgggcggt cgccaaattc gtgctcgata tcccgttcgt gttcgcaacc
     1801 accgcgctgt tgttcacaat tgcgggaggc gcgctgggca cactcattct gggactttcc
     1861 ggcggcttcg ccgcgcttgc cgccaagcct gccgcgcggc tgcgcaaccc ttaacggcaa
     1921 ttaatcgtga cgcggcacta tggcttgatg ttggaggcgc gaagcccgat attagacggg
     1981 atcctcgtga tcagactttt ccaggagatt cgcccatatg gctgagtacg acaatcgggt
     2041 tcggagttgg ggtggcgccg atgccaccgc catcgatgtg ggtcttcggg cctatatgct
     2101 ccgtgtctac aattacatgc tgatggggct tgggctcacc ggcttgaccg catggttcac
     2161 actgagcacg cccgccttcg ccaatctgtt tttcaccgtc acggcgcggg gctatacctt
     2221 ggcgccgctt gggtgggtgg tactgcttgc ccctctgggc ttggtcttct tcctcagttg
     2281 gcgcatccag caaatgagtt tcgccgccgc tcagatgacg ttctgggtct acgccggtct
     2341 gatgggcatc ggccttacgc cggtgttgta tctctatacc ggcgcctccg tggagcaggt
     2401 cttcttcgtc accgccgcca cattcggggc catgagcctg tggggctata cgacaaagcg
     2461 cgatctgtcg ggcctcggtt cgtttctatt catggggctg atcggtctca tcatcgcttc
     2521 actggtcaac atgttcatgc agtccagcat gatgcagtgg gtgatctcgg tgatgggcgt
     2581 gctgatcttc accggcttga ccgcctatga cacgcagtcg atcaaggaaa cctattacgt
     2641 cggggacgac ggcgcggtgg caggcaagaa ggctgtgatg ggtgcgttgc gcctctatct
     2701 cgacttcatc aacctgttca tcatgctgct ccgcctgatg ggggaccggc gctgatcttc
     2761 agcccctgca ataaaacgcc tgcaataaaa aaaccccgga aagcatcgct tccggggttt
     2821 tttgtttgcc gcgtttaaga tcaggtcatt gtccctctgc cgcgcatgcc gcccatcagc
     2881 aacgcaatgg caaacagcgc aaggaagaca aagaacaaaa tcttggcgat cccgatcgac
     2941 gcgccggcaa tgccggtaaa cccgaacagg gccgcgatca atccgagcac cagaaataca
     3001 agggcgtaat ggagcataac aatctcccga ttcagcttcg ttatggaaac gccccggcag
     3061 gctcaaggtt ctcgggtaga atccactatt cgggaaaatg gcccaaagcg ggcagaacaa
     3121 atccctgtgt taacttgccg taatgtctta gcccaggcct ctgaggctct cgcttccgcc
     3181 ggcgcttttc gcccggactt ccgtttattc cgcagcgacc gcgcgcaata gcttgcggtc
     3241 cagcacgcgc agcacttgct cgaggtcggg cccgcgtttg agcacctgcc cgcccgctgc
     3301 gaccacgcaa tattgtccct gccgctcttt cagtttcggg cgcttttcga tggaatagag
     3361 aggcatctcg ctgctgcgcc ggaaaaccga gaacaccgcc acgtcctcgc ggaagtcgat
     3421 ggcgtagtcg cgccattcgc ccgccatcac catgcggcca taggtgttca ggattcgctt
     3481 gagctcggac ctgtcgaaac gaatgcgcgt atcgcgcgtc tccggtccac tcgccggacc
     3541 attgtccgaa accgtgcccc tggatgaggt ccgtgcgaag tgaggccctt tcgcacggcc
     3601 gaaaactata ggttcgtcca ccatcttgcg aagcatggcc tctcagacgg cggcggacaa
     3661 gggcctaggc gccgcagcac gtgcgtcctc atgcaaggag cctcacgagc cgttgatcgg
     3721 cgccacgaaa atgccgcatt ggggacaagc gtgcgccgct ttggtccccc atgttcttca
     3781 tagtcggctg gcgagaaagc ctgaccggga gttacccagc cccccagccc ctgccggacg
     3841 ggaagtccgc cagccgactt ttccaagcaa gctttcagag acaattcctc accggcccgc
     3901 gttgggccgg tatctttttg ggccggtgtc tttatagagc cttgcgctga cgaacccttt
     3961 ttggcgattt gccgccctcg ggacgttttt tcggctatag ctcctcgcgc aggcgtgaat
     4021 catctgtaat cgccagactg caggaagccc ttgcagcgga caggcgattt ttctaaggga
     4081 acagacccac gcgttcaggg tcattcaagc cacctgctgc catgatcata ctcaatggac
     4141 tgaccaagac gttcggctcc atcgccgcgg tttcaggaat agacctgagc gtgcggaagg
     4201 gcgaagtgct gggctttctc ggacctaacg gcgcgggcaa atccaccacc atgaaaatga
     4261 tcgcgggcta tttcacgccg acgtcgggac gtgtctccat ttgcggccac gatgtggaga
     4321 acgataccat cgcggcccaa gcccaactcg gctatctgcc cgaaggggcg ccggcctatg
     4381 gcgatatgac gccgagccaa ttcctgacct tcatcgccca tgcgcgcggc ttttggggcg
     4441 cggaggccaa acagcgcgtc aataaggcca cgcttctgac cgaactcgaa tccgtcatgg
     4501 agcagccgat cgagacgctg tccaaaggct tcaagcgccg cgtcgggctt gcacaggcga
     4561 tcctgcacga tccccccgtc ctcgtcatgg acgaaccgac cgacggcctc gatcccaatc
     4621 agaagcacag cgtgcgcaca ctgattcggg gcatggcggc cgacaaggcc atcatcatct
     4681 cgactcatct cctggaggag gtcgatgcgg tctgcacgcg cgctgtgatc atcgatcgcg
     4741 gccgcattgt cgcggacgga acgccggcga gcctgctggc gcgctcccgc caccacaatg
     4801 cggtgacgct cactctgcat aaagatcagg cagccgcggc caaggtggcc ctccaggggc
     4861 tgccaggcgt ggcttccgcg gaaacggttt ccgaggccga cgggctcgcc accatcaccg
     4921 cctatccgcg tgagaacgaa ctcatcatcg aaaatgtcag cacgctggtg gcggcgcaga
     4981 agtgggacgt caaggaactc tacgccgagg ccgggcgact cgacgacgtc ttccgcgcac
     5041 tgaccaccca tgacacgcag cgcgtccgcg agcgcaacgg agccgccgca tgagaccggt
     5101 ccttgccatc ttcaaacgcg aattcgccgc ctatttcgcc acccccctcg cttatgtgtt
     5161 catcgtgatc ttcctgctcg cgatgggggc gttcacattt tatgtcggtc atttctacga
     5221 caatggcgtg gccaatcttt cggtcttttt cggctatcac ccttggcttt atctcttcct
     5281 ggtgccggcc gtcggcatgc ggctctgggc ggaggaacgc cggctcggca ccatggaact
     5341 cctgctgacg cttccaatcc cgatttgggc gagcgtgctc ggcaaattcc tcgccgcctg
     5401 ggcctttgtg ggcgtagcgc tcgccctcac cttcccgatc tggatcacgg tcaattatct
     5461 tggcaatccc gacaatggcg tgatcgtcgc gagttatgtc ggcagcttcc tgatggcagg
     5521 gggctatctc gcgatctcgg cggcgatttc agccacgacc aacaatcagg tcatcgcctt
     5581 cgtggtgagc gtggtggtgt gcttcctctt caccatctcg ggcgcgccct tggtgttgga
     5641 tgtcttccag gcctgggcgc ccatcgtgct ggtgaacacg atctccagct ttagtttcct
     5701 gacccatttc caggcgattt cctcgggcgt gatcgatctt cgcgacctca tctatttcgg
     5761 gtcgctgatc gcgctgttcc tatcggtgaa tgtcgtgctg gtcgacctca agaagacgaa
     5821 ctgagatgaa accgctgtcc catcgcgtgt tcgctctctc ggccatcgcg ctcgccgtgg
     5881 tgctgttcgt ggccatcaac atcgtcgcca atcaatggct gggcacagca cggctcgatc
     5941 ttacccagaa cgggctctac accgtgtcgc cgggcaccga ggccatcgtc tccaaattgc
     6001 cggagccggt gacgctgcgc ttttattttt cacggcagtc ctcggccgga tatgcgcaga
     6061 ttgtcgccta tgcgggccgc gtgcacgatc tcctgcagga atatgcggcg ctttcccacg
     6121 gcaagatcat cctggaagaa ctggacccca cgccctttac gcaggccgag gacgatgcgg
     6181 tcgctcaagg cctgaccggc gcgccgactc aggaaggcga caatgtctat ttcgggcttg
     6241 tcggtaccaa cacgctggcc ggccacgagg tgattccgtt cttcgatcag tcacgcgagg
     6301 aatatctcga atatgatctg acctcgatga tctacaagct ctcgcagccg gcgaagccga
     6361 aactcgggat cctgtcggcg ctgccgctgg aacagggagc gggcggcctg caggcggcgc
     6421 tggccgggaa cgcgcagccc tatgcgatct atcagcagct ccgcgacaat tacgacgtgc
     6481 agatgctgga cccgatgacc accgatcgca ttcccgccga tgtgggcacg ctgctcgtgg
     6541 ttcatccggc aaatttcacc agctcggtgc agtacgccat cgaccagttc gtgctgaagg
     6601 gcggccatgc gatcgtgttc gtcgatccgc tgtcggaatc ttcggtgcag cagggaccgg
     6661 gcgcccagga tcaaggcgat aaatccagtt cgacactggg gccggtgttc acagcgtggg
     6721 gtctcgatta cgacccgacc aaggtggtcg ccgacgccga cctcgcccag gccgttcagg
     6781 tcgccggtcc caacggccag ccgcgggtga tcgattacat cgcgtggctg cggatgatgc
     6841 ccgcgaattt cgaccgtaac gatccgatca cctccaatct gcaggtgctg aacatcgcaa
     6901 gcgcgggctc cctcaagccg catcagggcg cgacgacgaa gttccagccg ctcctgacca
     6961 gttccacgac ggcggcactt ctggattcca tcctggtgcg gacggtgcag aacccttccg
     7021 atctgctgcg ccggttctca cccacgggcg cgcgtttcac catcggcgcg cggatcacgg
     7081 gtccggccaa gaccgcgttc ccgaacggcg ctccggccgc gccgcccaag cccccggccg
     7141 attcgaacga accgcccaag cccgatgccg gcgcgctccc cgctcagatc aaagaggcga
     7201 agaacatcaa cgtcgtggtg atcgccgaca gcgatctgct cgatgaccgc ttctgggtgc
     7261 aggtgcaaaa cgtcgtcggc cagaggatcg cggtgcccac cgccgacaat ggcgcgctgg
     7321 tgatgaacgc ggtcgaaaac atgatgggct cgaacgactt gatctcgctg cgcacgcgcg
     7381 agcgctcggc gcgccccttc gtggtggtgg aacagatccg gcggaatgcg gaaactcgat
     7441 tcctcgccga ggaacagcag cttcagcaaa aggtgacggc gaccgaagcg tccttgcgcg
     7501 cgctccaggg ccagaaccag ccgcagggcg gcgcggcgcc cgtgctctcc aaggaacagc
     7561 aggcccagat cgacaaattc cgccgcgatc tcgtgacgac acgcacgagt ctgcgcggag
     7621 tgcaggccag tctgcgtcag gacgtcgagc ggctgggcac gatgctcgct ttcctgaata
     7681 tcgcgctggt accgattggg atcggcgccg ccgcgatcgg gctttcggtc ctgcggcagc
     7741 gccggcgcag aaaagcaaag gccctcacat gagcgacatg gccatgagtg cgcggcagga
     7801 cccacggcgc cggagcctca tgatcctcgg cggcgtcacg gcgttgttcg tcgtgctcgc
     7861 ggtggccgcc gtgctgcagc aagcctcctt ggtcgcgccg aaattcgcgg agcgtccgtt
     7921 cttccccggg cttaacgaca gcatcaacaa tgtgggcgag atcgccatcg cgtcgaagac
     7981 cggcacgttc catgtgcggc tgaaccaggg caaatggtcg gtggtcgaaa agggcggctt
     8041 tcccgccgat gccccgcagg cgcgcagcgt cgcggccggc atggcaggat tgaccgcgct
     8101 ggaaccgaag acgaatcgcg ccgattggct caaatatgtc gacctggacg cgcccgataa
     8161 gggcggcaac gcgattgacg tgaagctgac cgacaacatg ggcaagccca tggccgaaat
     8221 cctggccgga aagaatcagg gggccgccga cgatctgggg cgtacgcagc tctatgtgcg
     8281 caagccggac gaaacccagg cctggctggt gcgcggcaat ctcacgccaa agccgaacgc
     8341 ggccgattgg ctggataaga atgtcatcaa catcgcgcgc gaccgggtca aaggtgcgac
     8401 ggtcacgccc ccgatggggc cggcctatac gctctcgcgc gacagcaagg accaacagga
     8461 tttcaagctg ctcgatatgc cggcgggccg ccagctttcg ttcgaaggct cgcctgacgg
     8521 ggtgggcagc gcgctcatcg gcttcgactt cgacgatgtg gccaaggccg attccatcga
     8581 tttcagcaag gcgccgcaat cggtgaccca caccttcgac ggtctggaca tcacggtgaa
     8641 gatcgcgacc aaaggcatgg atcgctgggc cagcattgcg gcggcgggca ccaatccgat
     8701 gacgcagaat gaagccatgg gcatcgatgc gcggctttcg ggctgggcgt tcaagcttca
     8761 gccatcgaaa gccgatcagt ttgtggccac gcgcgagacg ctgctcaagc cccccggtgg
     8821 cccggcgact cccgcagccg cgccgggacg cgccccggcc tttccgtcgc gctgacgtct
     8881 tcgctccccc tttcggggga ggagattctc acgtaagcag cttcgcgtcg tttggggcgc
     8941 accccgtatt ggccgaagaa ccgctttatc tgcgcaatca atggtacatg gccgcactgt
     9001 cttcctcctt gaagacgggg gccctgcgcc gcgagatgct gttggccgag ccggtgctgc
     9061 tcggccgggg acgcgatggc ctggtattcg cgctgcgcga catctgcccc catcgtggcg
     9121 ttccgctttc ggcgggcaag attctgccgg agaacaccgt gcagtgcccg tatcatggct
     9181 ggcgcttcaa gcccgacggg gtgtgctcgc tgatcccttc gaccgtggag ggccaggacg
     9241 gtatcgatcc ctccaaggtc cgcgtgcgct cctatccctt gcgcgagcag gacggcctga
     9301 tctggattta catggccgcc aatgagcgcg acgcggcatc gccgagcgag ccgccgcgtg
     9361 tgccccttgc caacgcgacg ccgcgctggg tggagagcca gatgtttccc tgcggcatcg
     9421 atcatgccgt ccttggcctg atggaccccg cgcatgggcc ctatgtccac gcccattggt
     9481 ggtggcacaa ggcaccccgc gagaaggtga agcactacgc gcccttgccg aacggcttcg
     9541 tgatgacccg gcacaagcct acgaaaccag tctattccat gatcggggat gtctcgaccg
     9601 agatcacctt cgagctgccg agcacgcgct tcgagaatat ctcgggaaaa ctgttcggcc
     9661 ggcccatcga cgtgatcggg ctcacggtct gcaccccgcg tgacgcggag acgacacagg
     9721 tgacacagat cttctattgg ccggcctggc tcggtttcat ccggccgttt ttcatggcgc
     9781 ttggccccac cttcctgggc gatgaccgca agatcgttgc gcttcagcga gagggcctga
     9841 aattcaaccc gccactgatg ctgattcagg actccgatgt gcccgccata tggtaccacc
     9901 ggctcaagaa ggcctgggcg gaatcggtgg aaacgggcaa gcccttcgag aatccggtgc
     9961 aggagcgcac acttcggtgg cgaagctgag cataagccct ggtggggggt tgcggcgatg
    10021 cggcagactc tctagacgat ccgcttaacc ttcaatctgg cggtcccgaa gcgcttcccc
    10081 ggcgcgggcc tgcgtacgcg cgagtgacga tgccgaagcg gaccgacatc cacacgatca
    10141 tgatcatcgg agccgggccc atcgtcatcg gccaggcctg tgaattcgat tattccggcg
    10201 cccaggcctg caaagcgctt cgggccgagg gctatcgcat cgtgctggtg aattcgaacc
    10261 ccgcgacgat catgaccgat cccggcctcg ccgacgccac ctatatcgag ccgatcacgc
    10321 cggaattcgt cgagaagatc atcgtccgcg aaaaggcgaa cgtaccggcg ggacgcatct
    10381 ttgcgctgct gccgaccatg ggcggccaga ccgcgctcaa caccgcactc gatctttacg
    10441 aaagcggcgt tctggcaagc cacggaatcg aaatgatcgg cgccaaggcg ggcgccatcg
    10501 ccaaagccga agaccgccag ctcttccgcc aggccatgga gagcatcgga ctggaatcgc
    10561 cgcgctcggt tttcgtcatg gaccagaaag aagcactggc cgcgctcgaa cgcatcggcc
    10621 tgccggccat catccgcccc tccttcacgc tgggcggcac cggcggcggc atcgcctaca
    10681 atcgcgaaga attcgccgaa cttgccgcgg gcgcactcga agcctcccgg ggccacggca
    10741 tcctgatcga ggaatccgtg ctcggctgga aggaatatga gatggaggtc gtgcgcgacc
    10801 gcgccgacaa cgccatcatc atctgttcca tcgagaacat cgatccgatg ggcgtgcaca
    10861 ccggcgattc catcacggtc gcgccggcgc tgaccctgac cgacaaggaa tatcagcgca
    10921 tgcgcaccgc ctccatcgcg gtcctgcgcg aggttggcgt cgagaccggc ggatcgaatg
    10981 tacaatttgc actgaatccg gcagatggcc gcatggtcat catcgaaatg aacccgcgcg
    11041 tctcgcgttc ttcggcactc gcgtccaaag cgacgggatt tcccatcgcc aaggtcgcgg
    11101 ccaaacttgc cgtcggctac acgctggatg agctcaccaa cgacatcacg caggtgacgc
    11161 cggccgcctt cgaaccgacc atcgactatg tcgtcacgaa aattccgcgc ttcgcgttcg
    11221 agaaatttcc cggcgcagat cccatcctca cgacctccat gaaatcggtc ggcgaagcaa
    11281 tggccatcgg acgcaccttc gccgaatcgc tgcagaaggc gctgcgttcc atggagaccg
    11341 gtctcacggg actggacgag atcgccatcc ccaatcttgg tcagggcgat gacgccaacg
    11401 cgatccgcgc cgccctcggc cgcccgacac cggaccggct gcgcgtcgcg gcgcaagcgc
    11461 tgcggctggg tatccgcgaa gaggagctga cgcgcatcac cgcttacgat ccctggttca
    11521 taggcgaaat cgcgcaaatc ctgcgcaccg aggccgccat tcgcgcatcc ggtctgccca
    11581 aggacgccga tggattgcga cggctgaaag cgatgggctt ttccgacgcg cggcttgcga
    11641 cgctcaccgg cctgaaagag agcgacgtcg ccatggcacg cgcgcatctc ggcgttcacc
    11701 ccgtctacaa gcgcatcgat acgtgcgcgg ccgaattcgc ggccctgacg tcatatatgt
    11761 acgcgacgta tgaaacgccg gttgacggcg aggccgaatg cgaagcgcgt cccagcgagc
    11821 ggcgcaaagt catcatcctc ggcggcgggc cgaaccgtat cggtcagggc atcgaattcg
    11881 attattgctg ttgccatgcg tgttttgcgc tctccgccga gggttatgaa accatcatgg
    11941 tcaattgcaa tcccgaaacg gtgtctacgg attacgacac ctccgaccgg ctctatttcg
    12001 agccgctgac ggccgaagac gtgcttgaga tcatccggct cgaacaaacc aaaggcacgc
    12061 tcgcgggcgt gatcgtgcaa ttcggcggcc agacgccgct gaagctcgcg ggcgctctcg
    12121 gacaggccgg aattccgatc ctcggcacct cggccgatgc catcgacctg gccgaagatc
    12181 gtcagcgttt cctcgaattg ctcaagacgc tcggtctgaa gcagccgcag aacggcacgg
    12241 cactcgacat cgataccgcc gtcaatgtcg cccgtggaat tggctatccc acgatcctgc
    12301 gtccttctta tgtgctgggc gggcgcggca tgatcatcgt cgccgacgag cccgcgctga
    12361 aagccgccct gcaatcgggc gagcttttcc gtatctcggg cgccaacccg gtgctgatcg
    12421 acgggttcct gcagcacgcg acggaagtcg atgtcgacgc catcgccgat gagaccgggg
    12481 acgtgttcat cgccggcatc atggagcaca tcgaggaggc gggaatccat tccggcgatt
    12541 cggcgtgtgc actgccgccc cattcgctcg acgccgcgac gatcgccgaa ctcgaacgcc
    12601 agaccgtcgc gctggcccgt gcccttcaag tgcgcggtct catgaacgtg caatatgcga
    12661 tccagaatgg cgagattttc gttctcgaag tgaacccgcg ggcctcgcgc acggtcccat
    12721 tcgtcgccaa agcgatcggg cttccggtgg ccggaatcgc cgcgcgcgtc atggccggcg
    12781 aacaccttgc cggaatggcg ctggcgagag cgaaaaaatc gcacatcgcc gtcaaggaag
    12841 ccgtgcttcc ctttgcccgc tttcccggcg tcgatacggt ccttgggccc gaaatgaaat
    12901 ccaccggcga ggtgatgggt ctggacaccg atttcggccg cgctttcgcc aagagccaga
    12961 tcggcagcgg ggccaagctg ccgctttccg ggaccgtatt tatctccgtg cgggatgccg
    13021 acaaagccgg ggcatccggg cccgccgcac agctcatcgc catgggtttc gacatcgtgg
    13081 ccacgcgggg aaccgcaaaa taccttcagg cttcaggttt gccggtccgc attgtcaaca
    13141 aggtcctgga aggacgtccc cacatcgtgg acgcgatgaa aaacggcagc atctctctcg
    13201 tcttcaacac caccgatggg actcaggcgc tgtcggactc ggcctccatc cgcaagaccg
    13261 cgctgcagat gaagattcct tactgcacga ccatggccgg ggctgccgcg gtcacccagg
    13321 cgatccattc cttgaagtcg ggctcccttg aagtcgcccc cctccagtcc tatgcttaag
    13381 agctggcttg agccaatgtg gcgaaaccga agccggcaac ggtgacaaat ctcaagcgtt
    13441 gagttgagcg ttctgggtgt ccgttcttta gaacggctgg ccggagccag gaaattctgt
    13501 cgagtcaagg tggaagagga tggaacgtat tccgatgacc gccgcgggct acaaggccct
    13561 cgaggatgaa attcagcacc tgaagctgga ggagcgtcct gccgtcatca aaatgatcac
    13621 cgaagcgcgc gctcacggcg acctttcgga aaatgccgaa tatcatgccg ccaaggagcg
    13681 ccaggccttt atcgaaggcc gcgtgatgga cctggaggac aagctttcgc gcgccgacgt
    13741 gatcgacgtg tccaagcttt cgggcaagac cgtgaaattc ggcgcgactg tcacgctcgc
    13801 cgacgaggac aatgattcca aggtgaaata ccagatcgtc ggcgatctgg aagccgatgc
    13861 gaagcatggc cgcatttcca tttcctcacc gatcgcgcgc gcgctcatcg gcaaggctgc
    13921 tggcgatacg gtggaggttg ccgcccctgg cggcgcgcgt tcctatgaaa tcctgaaagt
    13981 gcaattcgcc tgaacctcga gatgaggaaa acgatgtcca aaaggcttgt cttcgccggg
    14041 gtcgtcgcag ttgcgctggc tggttcggat gcggcgcgtg cgatcgagat caatgtctat
    14101 tccgcgggcg cggtgcagga aggcgaacgc gcgctggccg tg
//
LOCUS       DASYEG010000133        15599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4967614, whole genome shotgun sequence.
ACCESSION   DASYEG010000133 DASYEG010000000
VERSION     DASYEG010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 15599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15599
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4967614"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VGM72_05750"
     CDS             <1..525
                     /locus_tag="VGM72_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4264802.1"
                     /translation="YALAAQYPQRITKWAVIDAPLPGVGNWAAQLSNPKTWHFNFHGP
                     DEERLVAGRERIFLDRFWNELSDDPRRIDEQTRAHYSAFYARPHAMHDALEQFVAFPQ
                     DGIDNRAFLAKGKLMMPVLAVGGAKSYGAGMETELKFVATNVKAGIVPDSGHWIMEEN
                     PAATTKLVMDFLAQ"
     gene            537..770
                     /locus_tag="VGM72_05755"
     CDS             537..770
                     /locus_tag="VGM72_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264803.1"
                     /translation="MRFDALTLFGVVSLTIMLVCYALERRNAWFTFGMGMSCMAASFY
                     AFLQGTWPFGILEAVWGAIAFSRWWRDRASLPG"
     gene            complement(756..1088)
                     /locus_tag="VGM72_05760"
     CDS             complement(756..1088)
                     /locus_tag="VGM72_05760"
                     /inference="COORDINATES: protein motif:HMM:NF036463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5335 family protein"
                     /protein_id="HEY4264804.1"
                     /translation="MAQALDKSQWAHYCDRLSDVLESSNAEIEVASLDLGNQIEAEWL
                     PFHGITYDDKDDIIDIELEGLDHIINHPQALRIDEDFAGLTTLEIKDGDGIQHVVRLK
                     EALALPRQ"
     gene            complement(1161..1772)
                     /locus_tag="VGM72_05765"
     CDS             complement(1161..1772)
                     /locus_tag="VGM72_05765"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEY4264805.1"
                     /translation="MERLIEGYRRFRSAQWPERRATYQALARDGQAPEAVVIACSDSR
                     VEPAVIFGAAPGELFIIRNVANLIPPYAPDGASHATSAALEFGVRVLQVKHLVVLGHA
                     MCGGITALLKGLPYPAGEFLGPWMHNAEQALDNAFADATVDPQTACELEAVKLSLSNL
                     LTFPWIRERVSSEELRLHGATFDIRSGVLSVLQPDGRFTPAEV"
     gene            complement(1791..2051)
                     /locus_tag="VGM72_05770"
     CDS             complement(1791..2051)
                     /locus_tag="VGM72_05770"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY4264806.1"
                     /translation="MSLLGWIIFGLITGFIASRVVNRRGEGCILNVSLGIVGACVGGL
                     IFTSIGGRGITGFDLYSMFVAIIGAIVVLLVFHAVTGRKGLR"
     gene            2189..2527
                     /locus_tag="VGM72_05775"
     CDS             2189..2527
                     /locus_tag="VGM72_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264807.1"
                     /translation="MKTKNAILVGALMGAMLATPVMAASGEVCLQHNRILNLRAIDSR
                     TVLATDRNYHRYTIHMNAGCTGLDNAAAHLVFRTWQNLACVDRGDIIGVQAPGLGFVT
                     CSIDNIQAGG"
     gene            2532..2885
                     /locus_tag="VGM72_05780"
     CDS             2532..2885
                     /locus_tag="VGM72_05780"
                     /inference="COORDINATES: protein motif:HMM:NF039797.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HEY4264808.1"
                     /translation="MNTKRVLVTGVMMGATLASPVLAAENACLQSNRVWGWQALDDHT
                     LIVTDRNEKRYTVHLTGGCVGLDKYAASALVVSTRTSLGCIGQGDRIAFRAPGLGPLT
                     CVVTNVTDAPAAPGK"
     gene            2896..3315
                     /locus_tag="VGM72_05785"
     CDS             2896..3315
                     /locus_tag="VGM72_05785"
                     /inference="COORDINATES: protein motif:HMM:NF039797.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HEY4264809.1"
                     /translation="MNRAAALAIPAVLLVAFTPARATAPADPSHDARACLQNNRIWGW
                     RAVNEKTLIVTDLNDQPFLVQLGGGCVGLNDAIVALEFRTWTNLGCLRQGDRVSYRAP
                     ALGVMSCFVRNVQPYQPGLNDPGAGRQEHRYDSAGGG"
     gene            complement(3312..4307)
                     /locus_tag="VGM72_05790"
     CDS             complement(3312..4307)
                     /locus_tag="VGM72_05790"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEY4264810.1"
                     /translation="MTNPVLVEITRGPLVESIHRGAVAVADASGRLRLSIGDVERPVF
                     PRSALKPIQAVPLLESGAADAFGLSDEELALACASHSGEPRHTTRIAAWQERIGCSAA
                     DLACGPHRPMHEPTATAMIRIGEKWTPLHNNCSGKHTGFMTLARGMGADVAGYQDLRH
                     PVQRAVEQTLKEMAGILGPLPHGIDGCTVPNFALPLAALARAMAGFADPGALGSDRAK
                     VCARVLRAMTANPDLIAGTGRPCTLLMRQAPDIAVKTGAEGVYAAILPTLGLGVALKI
                     DDGAGRAAETAIAAVLIALGAVTDEAAAHALAHAPVLNTRGVTVGERRVPQGLFA"
     gene            4424..4969
                     /locus_tag="VGM72_05795"
     CDS             4424..4969
                     /locus_tag="VGM72_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY4264811.1"
                     /translation="MRGYFAVGTDGLSKPMNLGNLLRIAHAFGGSFFFTVAGRVKLAD
                     ALSDTSDAAGHMPLYQFGTAEELRLPQGCELVGVEITDDAVALPSFRHPLRAAYVFGS
                     ERLSLSEAMLARCRHVVKIPTRFSINVGMAGAIVLYDRMLTLGTFAERPLRAGGPAAA
                     EIPPPVRWGKPIMRRRSSPSV"
     gene            5033..5566
                     /locus_tag="VGM72_05800"
     CDS             5033..5566
                     /locus_tag="VGM72_05800"
                     /inference="COORDINATES: protein motif:HMM:NF018482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEY4264812.1"
                     /translation="MSPISIAIAFAGMLMALTAAHTALAAEEKPTLLESFRDWHVYQT
                     GKGPNRLCYALSEPKQMDPKNVKRDSAFFLISTWPGRKVRNEPSVVPGYAYKDGTKTD
                     VQVGSDKFSFFTKNDGSAGGAWLEDSKDEKKLIDAMKKGSGMNITGISSLGTMTHDTY
                     SLAGISAALDKLDSSCK"
     gene            5628..6755
                     /gene="rlmN"
                     /locus_tag="VGM72_05805"
     CDS             5628..6755
                     /gene="rlmN"
                     /locus_tag="VGM72_05805"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY4264813.1"
                     /translation="MNSGLPIIGLSVVELTAALEAIGLEPKPARMRARQLWNWIYVHG
                     ARDFAKLTNLAKDFRELMAGNFSLARPEIVTQQISEDGTRKWLLRTGPGIEFETVYIP
                     EEDRGTLCVSSQVGCTLNCRFCHTGTQKLVRNLTPYEIAGQLMVARDALEDWPSTREN
                     RRVTNVVMMGMGEPLYNFDHVKTALGIVADGDGLALSKRRITLSTAGVVPMIPRAGAE
                     IGSSLAISLHAARDEIRDVIVPLNRKYPIAELMAACRTYPGVSNARRITFEYVMLKGV
                     NDAPADARALVKLVSGIPAKINLIPFNAWPGAPYECSDWGTIEAFAEIVNRAGYASPV
                     RTPRGRDIMAACGQLKSASLKERASDRMAALRSAELARMPE"
     gene            6802..7536
                     /locus_tag="VGM72_05810"
     CDS             6802..7536
                     /locus_tag="VGM72_05810"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HEY4264814.1"
                     /translation="MSAYPPFAPSTDTPPELNPLLDRARLAEDFRSTRRIHIPNVLTE
                     PSAARLFRALQQETPWTVTFNKGADFLDVENVSNEERARLSRAAFERARSPGFNYIYD
                     NHRLSRNGEPYADPMHYLARVVTFLNAPHVLQFMREVTGLFDIAWADAQATLYRQGDF
                     LTAHDDKTGGHKRLAAYVLNMTPGWRHDWGGILQFIDERGHIAEGFVPTYNALNVFQV
                     PILHAVSQVAFYGGFRYSITGWFHGR"
     gene            complement(7545..8255)
                     /locus_tag="VGM72_05815"
     CDS             complement(7545..8255)
                     /locus_tag="VGM72_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264815.1"
                     /translation="MSQPPAKRGRIRPGAVLLFLAGICGFLTVPFFLERAASSPAPEA
                     SVAGPAAVRPIQVRTPDGQTIGWLQISYPESIRQNEEQQLDVKYIGDKGKWGRVKDIA
                     AMSVLIQAANLGLFPEPFEYRFNLARILRTGEDSHVWTVSPKQEGDYRLLVKFNVEPD
                     SFTVLPVAVNGIDVSTNGETSLPVRVNTRYDVPQAWVDGLKAFGYVLSFILTLPLLKT
                     FVERSWNRGRSGVAGPGV"
     gene            8407..9639
                     /locus_tag="VGM72_05820"
     CDS             8407..9639
                     /locus_tag="VGM72_05820"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEY4264816.1"
                     /translation="MAKKDVKKVVLAYSGGLDTSVILKWLQTEYEAEVVTFTADLGQG
                     EELEPARAKAQLLGIKPDNIFVEDLREEFVRDYVFPMFRANALYEGVYLLGTSIARPL
                     IAKKQIEIAEKTGADAVAHGATGKGNDQVRFELAYYALKPDIKIIAPWREWDLTSRTR
                     LLAFAEAHQIPIAKDKRGEAPFSVDANLLHSSSEGKVLEDPSVEAESIVYQRTLSPEE
                     APDKATTVEIGFEKGDAVSIDGAPLSPATLLTRLNALGRANGIGRIDLVENRFVGMKS
                     RGIYETPGGTILHAAHRAIESITLDRGAAHLKDELMPRYAELIYNGFWFSPEREMLQA
                     AIDKSQEYVAGTVRLKLYKGNVMVVGRSSSNSLYAQDLVTFEDDQGAYDQKDAAGFIK
                     LNALRLRTLGQRKRKTKT"
     gene            9636..10478
                     /locus_tag="VGM72_05825"
     CDS             9636..10478
                     /locus_tag="VGM72_05825"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4264817.1"
                     /translation="MSGPDNASQIAYWNGPNGEAWAKVREKRDSDHAFLTAALIDRAQ
                     PRPGEHVLDIGCGSGTTTLKLAELVQPKGYVLGVDISRPMLGVARRRGVAAKSGAQFL
                     EEDAALCDFEPQSFDLAFSQLGVMFFANPVAAFRNVHRAMKPGGRLHFACWRHPFEHL
                     WAFVPESAAKPFLPQSPPADPNAPGRYAFQNPDHVKSVLLQAGFLAPEFEKLDARNFA
                     GATPEAAAASLIEAGPLQRTLTGMDDATRARVRAAVTERLAREMGPGGIYLTSAAWLV
                     RARA"
     gene            10519..11265
                     /locus_tag="VGM72_05830"
     CDS             10519..11265
                     /locus_tag="VGM72_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264818.1"
                     /translation="MRTSGKAFLAVALMFPYAEMAHAAAVEDANAGVVAARAGKYDDA
                     IELFTKAINSDELNLTGRAQAYAYRGIAKATLGDYDAAREDLNFAVALDSPYNADAYA
                     FRGYIALVTGAPQKAAADLAKSAGLKVWAYNALWLALARMRAGIADNGDVSLANNAMT
                     LDLKQWPGPVVKFLMGQAQTGEVAAAANEGDPAKLTERVCDVNFYIGEYDLSHNDAAA
                     AKPLLQDAAGNCPFASFERMGATAELMRLK"
     gene            complement(11269..11877)
                     /locus_tag="VGM72_05835"
     CDS             complement(11269..11877)
                     /locus_tag="VGM72_05835"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY4264819.1"
                     /translation="MMTLYFAPGSSSMAAHIALHEVGADFESRPMSFAKKDMQSPAYL
                     AINPEGKVPTLVIDGRPLTEVAGILVYLARKFPEAGLLPADDPETEAQAISWMSFTAS
                     TLHPARRRGLDYTRNVYRIADARLGNRQWALGRYSIADIHLFRLYWRFTRAVDVAPEM
                     FPHLAAHHERMMARPAVQRTFETESAIGYELPHWFPGQTPAA"
     gene            complement(11887..12366)
                     /locus_tag="VGM72_05840"
     CDS             complement(11887..12366)
                     /locus_tag="VGM72_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEY4264820.1"
                     /translation="MSAYDFEFTAIDGTPLKLSQFAGHPVLIVNTASQCGLTPQYKDL
                     EALWRKYRDRGLVVLGVPSNDFGAQEPGTEAEIAEFCSTRYNVSFPMTAKNPVIGASA
                     HPFYKWAVTQAGEAAAPRWNFHKYLIGPKGELAGTFGSKVAPGDAALTDAIETALNA"
     gene            complement(12378..14828)
                     /gene="gyrB"
                     /locus_tag="VGM72_05845"
     CDS             complement(12378..14828)
                     /gene="gyrB"
                     /locus_tag="VGM72_05845"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012535740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEY4264821.1"
                     /translation="MAEQDNRPNPNGNGSSAQSYDADSIKVLKGLDAVRKRPGMYIGD
                     TDDGSGLHHMVYEVIDNAIDETLAGYADRIEAELNPDGSATVRDNGRGVPVDIHKEEG
                     VSAAEVIMTQLHAGGKFEQNAYKVSGGLHGVGVSVVNALSEWLDLRIWRDGDEHYMRF
                     RDGDPVAPLTIVGKANGRRGTEVTFLPSTKTFTKTEFDFATLEHRLRELAFLNSGVTI
                     RLIDKRGVEKKEVLLHYEGGLEAFVRYLDRAKTGLIPNPILIGADRDNMRVEAALSWN
                     DSYHENMLCFTNNIPQRDGGTHLAGFRAALTRVVTKYADESGIGKKDRVPLTGDDCRE
                     GLTCVLSIKVPDPKFSSQTKDKLVSSEVRPAVEGAVLEQLGRWFEEHPAEARGIVGKV
                     IEAATAREAARKARELTRRKGALDGVGLPGKLADCQERDPAKCEIFIVEGDSAGGSAK
                     QGRNRANQAVLPLRGKILNVERARFDKMLGSQEIATLITALGTGIGREEFNPDKLRYH
                     KIIIMTDADVDGAHIRTLLLTFFYRQMPELIERGHLFIAQPPLYKAARGKSERYLKDE
                     RELENHLIEEGIKDSALTLSGGERIGGADLAHLLERARAARTALDGFPRHYPRFVLEQ
                     AAIAGALNPEVLSDAEKAEEAASYIARRLDSMSEETERGWKGEPTPDGGLKFRREVRG
                     VTETIAIDGALIASADARKLDRMAVELQTTYLKASRLTRKDQLHEVRSPTQLLEAILG
                     WGRHGLALQRYKGLGEMNPNQLWETTLDENARTLLQVRVEHADAADDLFAKLMGDVVE
                     PRREFIQDNALNVVNLDV"
     gene            complement(14828..>15599)
                     /locus_tag="VGM72_05850"
     CDS             complement(14828..>15599)
                     /locus_tag="VGM72_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264822.1"
                     /translation="ELIPMLWLTPAMDRLFLEGASERRRFLDRLVFALDSTHAKRATR
                     YERAMHERLRLLRDGSRDRVWLDGLEETMATEGAGLTAARLSVIERLNGELEIRGMEG
                     AFPCAHLSLQDSLQGCANDPARLQSALAASRERDAEAGRTNAGPHLADLEVRHTAKRS
                     DARDCSTGEQKALLISIVLANAWIQKRRRDGIAPLLLLDEIAAHLDVQRRAALFAEIL
                     ALGSQAWLTGTDRSLFAPLQEHAAFFSIDAGRFVPMER"
BASE COUNT         2897 a         4854 c         4895 g         2953 t
ORIGIN      
        1 tatgcgctcg ccgcgcaata tccccaacgc atcaccaaat gggcggtcat cgatgcgccc
       61 ctgccgggcg tgggcaattg ggcggcccag ctcagcaatc ccaaaacttg gcacttcaat
      121 ttccatggac ccgacgagga acgtctggtc gcgggccgcg agcgcatctt cctcgatcgc
      181 ttctggaacg aattgtcgga cgatcccaga cgcatcgacg aacagacgcg cgcgcactat
      241 tccgcgttct atgcgcggcc ccatgcgatg catgacgcgc tcgaacaatt cgtggcgttt
      301 ccgcaggacg ggatcgacaa tcgcgccttc ctcgccaagg gcaaactgat gatgccggtt
      361 ctggcggtgg gcggtgcgaa atcctatggc gccggcatgg agacggaact gaaattcgtc
      421 gccaccaacg tcaaagccgg catcgtgccg gattccggcc attggatcat ggaagaaaat
      481 ccggccgcga ccaccaaact ggtgatggat ttcctcgcac aataggaaga atcgccatgc
      541 gtttcgatgc gctcacgctg ttcggcgtgg tgtcgttgac catcatgctt gtctgttacg
      601 cgctcgaacg caggaacgcg tggttcactt tcggaatggg catgtcctgc atggcggcat
      661 cgttttatgc cttcctgcaa gggacttggc cgttcgggat tcttgaggcc gtctggggcg
      721 ccatcgcctt cagccgctgg tggcgcgacc gcgcctcatt gccggggtag cgccagcgcc
      781 tccttcaggc gcaccacatg ctggatgccg tcaccgtcct tgatctcgag ggtggtgagg
      841 cccgcgaaat cctcgtcgat gcgcaatgct tggggatggt tgatgatgtg atccaagcct
      901 tcgagttcga tgtcgatgat gtcgtccttg tcgtcataag taatgccgtg gaacgggagc
      961 cattccgctt cgatctggtt cccgaggtcg agggaggcca cttcgatctc cgcattgctg
     1021 gattccagca cgtcggacag acggtcgcaa taatgggccc attgcgattt atcgagtgct
     1081 tgcgccatgt ttgccgtcct tcagagattg cacggcgaaa accgattccg gccgggaatg
     1141 ttcccgtttt tttgcgcgtg tcacacctcg gcgggggtaa accggccgtc cggctggagg
     1201 acggaaagca cccccgagcg gatgtcaaac gtagccccat gaaggcgaag ttcttcgctg
     1261 gacacccgct ccctgatcca ggggaatgtc agcagattcg acagcgacag cttgaccgct
     1321 tccaattcgc aggcggtttg ggggtctacc gtcgcgtctg caaaggcatt gtccagcgcc
     1381 tgctcggcgt tatgcatcca gggaccgagg aattccccgg cgggataggg gagccccttc
     1441 agcagcgcgg tgatgccgcc acacatggca tggccgagca caaccagatg cttcacctgc
     1501 aacacgcgca caccgaattc gagagccgcg ctggtcgcat gcgaggctcc gtccggggcg
     1561 tagggcggta tgagattggc gacattgcgg atgatgaaca attcccccgg cgccgctccg
     1621 aaaatcaccg caggttcaac ccggctgtcg gagcaggcta tcaccaccgc ctccggggcc
     1681 tgaccgtcgc gggcgagcgc ttgataggtg gcgcgacgtt ccggccattg tgcgcttcga
     1741 aatctccgat atccctcaat cagacgttcc atatcgctcc tatatatctc ctatcgcagg
     1801 cctttgcggc cggtgacggc gtggaagacc agcagcacca caatagcgcc gatgatcgcg
     1861 acgaacatcg aatagaggtc gaagccggta atgccacggc ccccgatgct ggtgaagatc
     1921 aagccgccca cgcaggcgcc gacaatgccc agcgagacat ttaggatgca gccttctcct
     1981 cgccggttca ccacgcggct cgcgataaaa ccagtaatca ggccgaagat gatccagccg
     2041 agaagggaca taggccaaaa acctccgaat cgaccacaaa acttggtgat ctgcagggcc
     2101 ggcgtaacct ctgccggaac agcgcttccc atgatacgtt atgatatgaa gcgtaaacgg
     2161 aggaggggca actccgaagg atacggacat gaaaacgaaa aatgcaattc tggtgggtgc
     2221 gctgatgggc gcgatgctgg cgacgccggt aatggcggcg agcggcgagg tttgcctcca
     2281 gcacaatcga atcctgaact tgcgcgcgat tgacagccgc accgtgcttg ccacggatcg
     2341 caattatcac cggtatacga tccatatgaa tgcgggatgt acgggcctcg ataatgccgc
     2401 ggcgcatctg gtgttccgca cgtggcaaaa tctcgcttgc gtggatcgcg gcgatatcat
     2461 cggcgtgcag gcgccggggt tgggtttcgt gacctgctcc atcgacaata tccaagcggg
     2521 cgggtgaagg catgaatacg aagcgcgttc tggttacggg ggtgatgatg ggcgcgaccc
     2581 tcgcctcgcc ggtattggcc gcggagaatg catgcctgca aagcaatcgc gtctggggct
     2641 ggcaagcgct cgacgatcac accctcatcg tgacggaccg caatgaaaag cgctacacgg
     2701 tgcacctgac aggcggctgc gtcggactcg ataaatatgc cgcttctgcg ctggtggtca
     2761 gcacgcgcac cagcctgggc tgcatcgggc agggcgacag gattgcgttt cgggctccgg
     2821 gtttgggacc gctcacatgc gtcgtcacga acgtgaccga tgcgcccgcc gcgccgggca
     2881 agtgagcggt ttgcggtgaa tagagcagca gccctcgcga ttccggcagt cctgctggtc
     2941 gcattcacac cggccagggc cacggcgcca gccgatccat cgcatgacgc gcgagcgtgc
     3001 ctgcagaaca atcgcatctg gggctggcgc gcggtcaacg aaaaaacgct catcgtcacc
     3061 gatctcaatg atcaaccgtt tctggtgcag ctcggtggag gctgtgtcgg gctgaacgat
     3121 gcgatcgttg cactggaatt ccggacgtgg acgaatcttg gctgtcttcg tcagggcgat
     3181 cgcgtcagct accgggcacc ggctctgggg gtgatgagct gtttcgtgcg gaacgtccag
     3241 ccttaccagc cgggcctgaa cgatcccggt gccggacggc aggagcatcg ctacgacagc
     3301 gccggcgggg gctaggcgaa caatccctgg ggcacacggc gctcgccaac cgtgacgccg
     3361 cgcgtgttga gaaccggtgc gtgggcgagc gcatgggccg cggcttcgtc agtcaccgcc
     3421 cccagcgcga tcagcaccgc ggcaatcgcc gtttcggcgg cgcgtcccgc cccgtcgtcg
     3481 atcttcagcg ccacaccgag accgagtgtg ggcaggatgg cggcgtaaac cccctccgcg
     3541 ccggttttga ccgcgatatc cggggcttgg cgcatcagca gcgtgcaggg ccgtccggtc
     3601 cccgcgatca gatcgggatt ggccgtcatg gcgcgaagaa cgcgcgcgca gaccttcgcg
     3661 cgatccgaac ccagcgctcc cggatcggcg aatccggcca tggcgcgcgc gagcgccgcg
     3721 agcggcagcg cgaaattcgg cacggtgcag ccgtcgatgc cgtgcggtag cggtcccaat
     3781 attccggcca tctccttaag cgtctgttcc acagcgcgct ggaccggatg tcgcagatcc
     3841 tgatagcccg cgacgtcggc gcccattccg cgcgccagcg tcatgaagcc cgtatgcttg
     3901 ccggagcaat tgttgtgcag cggcgtccat ttctcgccaa ttctgatcat cgcggtcgcg
     3961 gtgggctcat gcatcggccg atgggggccg cacgccaaat cggcggcgga acagccgata
     4021 cgctcctgcc aggccgcgat ccgcgtggtg tggcggggct cgcccgaatg cgaggcgcag
     4081 gcgagcgcca gctcctcgtc ggagaggccg aacgcatcgg ccgcccccga ttcgaggagc
     4141 ggaaccgcct ggatcggttt tagcgccgaa cgcgggaaga ccgggcgctc cacatcgccg
     4201 atagaaaggc gcagccgccc gcttgcatcg gcaaccgcca cggcgccgcg atggatgctt
     4261 tcgacaaggg gaccgcgcgt gatttcgaca agaacgggat tggtcatggg caaggacgcg
     4321 taatccatac aacagtgttt gtcatcccgg gcggacgggt tagcgttgcg ctgccgtacc
     4381 caggactggg cgggatgaca gggcgggatg acagaggttg ggaatgcgtg gctatttcgc
     4441 cgtcggcacg gacggactct ccaagccgat gaatctcggc aatctcctgc gtatcgccca
     4501 tgcgttcggc ggcagcttct tcttcaccgt cgcgggccgc gtaaaactcg ccgatgcgct
     4561 gtccgatacg tccgacgcgg ccggccatat gcccctctac caattcggca cggccgagga
     4621 gctgcgcctt ccccagggtt gcgagctggt gggggtggaa atcaccgatg acgcggtcgc
     4681 tctgccgagc ttccgtcacc cattgcgggc ggcttatgtg ttcggttcgg agcggctgtc
     4741 gctttccgag gccatgctcg cgcgctgccg ccatgtcgtg aagattccca cgcgtttttc
     4801 catcaatgtg ggcatggctg gagccatcgt tttgtatgac cggatgctga cgctcggaac
     4861 attcgcggag cgtcctctca gggccggtgg gccggcggcg gctgagattc cgcctcccgt
     4921 gcgctggggc aaacccatca tgcgccggcg aagttcccct tccgtctagg tgcttcaact
     4981 tttggcccaa atcatgctct aagaccaatc gttgaacatt agagacattg cgatgagtcc
     5041 gatatcgata gccattgcgt ttgccggcat gctgatggcc ctgacggcgg cgcacacggc
     5101 cctggccgcg gaagaaaagc ccacgctttt ggaatcgttc cgcgattggc acgtctatca
     5161 gaccggcaaa gggccgaatc gtctctgcta tgcgctgtcg gaacccaagc agatggatcc
     5221 caagaatgtg aagcgcgatt ccgcattctt cctcatttcg acctggcccg gccgcaaagt
     5281 ccgcaacgag ccctcggtgg tcccgggcta tgcctacaag gacggcacca agaccgacgt
     5341 gcaggtaggg tccgacaagt tcagtttctt caccaagaat gacggttcgg ccggtggtgc
     5401 ctggctggaa gattccaagg acgagaaaaa gctcatcgat gccatgaaga agggttcggg
     5461 catgaacatc accggcattt cttcgctggg cacgatgacc catgacacct attcgctggc
     5521 cgggatttcc gccgccctcg acaagctcga ttcaagctgc aaatgagtcg catccattcc
     5581 ctcattgtca tggcccgctg atgcggacca tgacacaaag gttttgaatg aattcgggac
     5641 ttcccatcat tggtctatcg gtcgtggaat tgaccgcggc actggaagcg atcggcctgg
     5701 agccgaagcc cgcccgcatg cgcgcgcgtc aattgtggaa ctggatctat gtccatggcg
     5761 cgcgcgattt cgcgaaactg accaatctgg cgaaggattt ccgggagctt atggcgggga
     5821 atttcagcct cgcgcggccc gagatcgtca cccagcagat ttccgaagac ggcacgcgca
     5881 aatggctgct gcggaccggg cccggcatcg aattcgagac ggtgtacatc cccgaagaag
     5941 atcgcggcac gctatgcgtc tcctcccagg tgggctgcac gctgaattgc cgtttctgcc
     6001 acaccggcac gcagaagctc gtgcgcaacc tcacacccta tgagatcgcc ggccaattga
     6061 tggtggcgcg cgatgccctg gaagactggc cctcgaccag ggaaaaccgc cgcgtcacca
     6121 atgtggtgat gatgggcatg ggcgagccgc tctacaattt cgaccatgtg aaaaccgccc
     6181 ttggcattgt cgccgatggc gatgggctgg cgctttccaa gcgccgcatc acgctttcga
     6241 cggccggcgt cgtaccgatg atcccgcgcg ccggagccga gatcggctct tcgttggcga
     6301 tttcgctgca cgccgcgcgc gacgaaatcc gcgacgtgat cgtgccgttg aacagaaaat
     6361 atccgatcgc cgaattgatg gccgcctgcc ggacctatcc gggcgtctcg aatgcgcggc
     6421 gcatcacttt cgaatatgtc atgctgaagg gcgtgaacga tgcgcccgcc gacgcgcgcg
     6481 cactggtgaa actcgtctcc ggcattccgg cgaagatcaa tctgatcccg ttcaatgcct
     6541 ggcccggcgc accctatgaa tgctcggact gggggacgat cgaggccttc gcggagatcg
     6601 tgaatcgcgc ggggtatgcc tcgccggtgc gcacgccgcg cggccgcgat atcatggcgg
     6661 cctgcggtca gctcaaaagc gccagtctca aggaacgcgc ctcggaccgg atggcggcgc
     6721 tgcgctcggc cgaactcgcc cggatgccgg agtagccgtt cgatcttgcg ccacgctcac
     6781 gaatgcgcga gtgattgagc catgagcgcc tatccgccct tcgcgccttc caccgatacg
     6841 ccgccggagc tgaacccgct tctcgaccgc gcgcggctgg ccgaggattt ccgcagcacc
     6901 cggcgcatcc atatccccaa tgtcctgacc gagccgtccg cggccaggct gttccgcgcc
     6961 ctgcagcagg aaacgccgtg gaccgtcacg ttcaacaagg gcgcggattt cctcgacgtc
     7021 gaaaacgtct ccaacgagga gcgcgcgcgg ctgtcgcgtg cggcattcga gcgggcgcgg
     7081 tccccgggct tcaattatat ctatgacaat caccgcttgt cgcggaacgg cgagccctat
     7141 gccgatccca tgcattatct cgcgcgcgtg gtgaccttcc tcaacgcgcc gcatgtgctg
     7201 caattcatgc gcgaagtgac gggactcttc gacatcgcct gggccgatgc gcaggcgacg
     7261 ctgtatcggc agggcgattt tctcaccgcc catgacgaca agaccggtgg ccataagcgg
     7321 ctcgccgcct atgtcttgaa catgacgccc ggctggcggc acgattgggg cggcatcctg
     7381 caattcatcg atgagcgcgg gcatatcgcg gaagggtttg tgcccaccta caacgccctg
     7441 aatgtgttcc aggtgcccat cctgcacgcg gtcagccagg tcgccttcta tggcggcttt
     7501 cgctattcca tcaccggctg gttccacggg cgctgacgca gcactcacac acccggtccg
     7561 gcaacgccgc tgcgtccgcg attccaagag cgttccacaa atgtcttcag cagcggcaga
     7621 gtcaaaatga acgagagcac atagccgaag gccttcagcc catcgaccca ggcctgcggc
     7681 acgtcgtaac gcgtgttgac ccggaccggc agcgaggttt ccccgttcgt gctgacgtcg
     7741 atgccattca ccgccacggg cagcaccgtg aagctgtccg gttcgacatt gaatttcacc
     7801 agcaggcggt aatcgccctc ctgcttgggc gacacggtcc agacatgcga atcctcgccg
     7861 gtccgaagga ttcgcgccag attgaagcgg tattcaaagg gttccgggaa caacccaaga
     7921 ttggcggcct ggatgagaac cgacatcgca gcgatgtcct tgacgcggcc ccatttgccc
     7981 ttgtcgccga tatatttgac gtccaattgc tgttcttcgt tttgccggat gctttcgggg
     8041 tagctgatct gcagccagcc gatggtttgc ccgtcggggg tgcggacctg aatcggcctc
     8101 accgcggccg ggcccgcgac gctggcctca ggcgccggag aggaggcggc gcgttcgagg
     8161 aagaacggga ccgtcagaaa tccgcagatg ccggccagga acagaagaac cgcgccggga
     8221 cggatgcggc cacgcttcgc gggaggttgg ctcaaggacg gcatccgtca cgggcttcgg
     8281 aaaagagtgc gtcggcgctt tttacgccag acgcaggaaa tttgcacatc gctgcgcgct
     8341 gtaggcttcg catttgcacg gaaaagcccg atccattaga ccgcatctga gagcgtggag
     8401 gggtgcgtgg cgaagaaaga cgtgaagaag gtcgtcctgg cctattcggg tgggctggac
     8461 acctctgtga tcctcaaatg gcttcagacc gaatatgagg cggaggtcgt gacgtttacc
     8521 gccgatctcg gccagggcga ggagctggaa cccgcgcgcg ctaaggcgca actcctgggc
     8581 atcaagccgg acaacatctt cgtcgaggac ctgcgcgaag aattcgtgcg cgactatgta
     8641 tttccgatgt tccgcgccaa cgcgctgtat gagggcgttt acctgctcgg cacctccatc
     8701 gcgcggcctt tgatcgccaa gaaacaaatc gaaatcgcgg aaaaaaccgg cgccgatgcg
     8761 gtggcccatg gcgcgaccgg caagggcaac gaccaggtcc ggttcgaact ggcctattac
     8821 gcgctgaaac ccgacatcaa gatcatcgcg ccctggcggg aatgggattt gacatcgcgc
     8881 acccggctgc tggcgttcgc cgaggcccat cagatcccca tcgccaagga caagcgcggc
     8941 gaagcgccgt tctcggtgga tgcgaatctg ctgcattcct cgtccgaggg gaaagtgctg
     9001 gaagacccat cggtcgaagc ggaatcgatc gtctatcagc gcacgctgtc gccggaggaa
     9061 gcccccgaca aggcgacgac cgtcgagatt ggttttgaaa agggcgacgc ggtttccatc
     9121 gacggcgcgc cgctttcgcc cgcgacgctg ctgacgcggc tgaacgcgct aggccgcgcc
     9181 aacggcatcg gccgcatcga tctggtcgag aaccgcttcg tggggatgaa atcgcgcggc
     9241 atctatgaga cgccgggcgg cacgattctg catgccgcgc atcgcgccat cgaatcgatc
     9301 acgctcgacc gtggcgcggc tcatctgaag gacgagctga tgccgcgcta tgcggagctg
     9361 atctataatg gtttctggtt ctcgcccgaa cgcgagatgt tgcaggccgc catcgataag
     9421 agccaggaat atgtcgccgg gacggtgcgt ctgaaattgt acaagggcaa tgtcatggtg
     9481 gtgggacgct cttcgtccaa ttcactttat gcccaggatc tcgtcacctt cgaggacgat
     9541 caaggcgcct atgaccagaa ggatgcggca ggcttcatca agctgaacgc gctgcgcctg
     9601 cgcacgctcg gccagcgcaa gcgaaaaaca aagacgtgag cggccccgat aatgccagcc
     9661 agatcgccta ttggaacggc cccaacgggg aagcctgggc gaaagtgcgc gaaaagcgcg
     9721 acagcgacca tgccttcctg accgcggcgc tcatcgatcg ggctcagccg cggcccggcg
     9781 agcacgtgct cgatatcggt tgtggctccg gcaccacgac gctgaagctc gcagaattgg
     9841 tgcaaccgaa aggttatgtg ctgggtgtcg atatttcccg accgatgctc ggcgtcgcgc
     9901 gaagacgggg cgtggcggcc aagagtggcg cgcaattcct cgaagaggac gcggcccttt
     9961 gcgatttcga gccgcaaagt ttcgatctcg ccttttcgca attgggtgtg atgttctttg
    10021 ccaatccggt cgccgcattc cgcaatgtgc atcgcgcgat gaaaccgggc ggccgtctcc
    10081 atttcgcgtg ctggcgccat cccttcgagc atctttgggc tttcgtcccc gaaagcgcgg
    10141 ccaagccttt cctcccgcaa tctccgcccg ccgatccgaa tgcgccgggg cgctatgcgt
    10201 ttcagaatcc cgatcacgtg aagagcgtgc tgctgcaggc gggctttctt gcccccgaat
    10261 tcgagaaact ggacgcgcgg aactttgccg gcgccacgcc ggaagccgcc gccgcttcgc
    10321 tgatcgaagc cggtccgctg cagcgtacgc taaccggcat ggatgacgcc acgcgcgcgc
    10381 gggtgcgcgc cgccgtgacg gaacgcctgg cccgcgagat gggcccgggg ggcatctatc
    10441 tcacctccgc cgcctggctg gtgcgcgcgc gtgcctgatc tctgctagaa tgcgcgtcca
    10501 gctcggcgag aggacgtcat gcgcacatcc ggcaaagcat tcctggcagt ggcgctgatg
    10561 ttcccgtatg cagaaatggc gcatgccgcg gctgtcgaag acgccaatgc gggcgtcgtg
    10621 gcggcgcgcg ccggcaaata tgacgatgcc atcgaacttt tcaccaaggc gatcaacagc
    10681 gacgaactca acctcaccgg acgcgcgcag gcctacgcct atcgcggcat cgccaaagcc
    10741 acgctcggag attatgacgc ggctcgggag gatttgaatt tcgcggtcgc gctcgacagc
    10801 ccctacaatg ccgatgccta tgcgtttcgc ggctatatcg cgctggtgac gggtgcgccg
    10861 caaaaggccg ccgccgatct tgccaaaagc gcaggcctga aggtctgggc ctataacgcg
    10921 ctgtggctgg cgctggcgcg catgagagcg ggcatcgcgg acaacgggga tgtttcgctc
    10981 gcgaacaacg ccatgacact cgatctgaag cagtggccgg gaccggtggt gaaattcctg
    11041 atgggccagg cgcagactgg tgaggttgcg gccgctgcca atgaaggcga tccggcgaaa
    11101 ctcaccgaac gcgtatgcga tgtgaatttc tatatcggag aatatgatct ctcgcacaat
    11161 gacgcggcgg cggccaagcc attgctgcaa gatgccgccg gcaattgtcc gttcgcgtcg
    11221 ttcgaacgca tgggcgcgac cgcggaattg atgcggctga aataggcctc acgccgccgg
    11281 tgtttggccg gggaaccaat gcggcagttc atagccgatt gcgctttcgg tctcgaaggt
    11341 ccgctgcacc gccggacgcg ccatcatgcg ttcatggtgc gcggcgagat gggggaacat
    11401 ctccggggca acatccacgg ctctggtgaa ccgccaatag aggcggaaga ggtggatgtc
    11461 cgcaatggaa tagcggccca acgcccattg gcggtttccc agccgcgcgt cggcgatccg
    11521 gtatacattc ctggtgtagt ccagcccccg cctgcgcgcg ggatgcagtg tggatgcggt
    11581 gaaggacatc cacgaaatcg cctgcgcttc ggtttccgga tcgtccgccg gcagcagacc
    11641 ggcctcggga aatttccgcg cgagatacac gaggatgccg gcgacctcgg tcagcggccg
    11701 cccgtcgatg acgagcgtgg gaacctttcc ttccggattg atggcgagat aggcgggcga
    11761 ctgcatgtcc tttttggcga aggacatggg gcggctttca aagtccgcgc cgacctcgtg
    11821 cagtgcgata tgagcggcca tcgaactcga gcccggcgca aaatacagcg tcatcatggc
    11881 ggtggcctaa gcgttcaacg cggtttcgat cgcatcggtt agtgccgcat cgccgggcgc
    11941 gaccttcgat ccgaacgtgc ccgccagttc gcccttgggg ccgatcagat atttgtggaa
    12001 gttccagcgc ggtgccgcgg cctcgccggc ttgggtcacc gcccatttgt aaaacgggtg
    12061 cgcgctggcc ccgatcaccg gattcttcgc cgtcattggg aagctgacat tgtagcgggt
    12121 ggagcagaat tcagcgatct cggcttcggt tccgggctcc tgcgcgccga aatcgttcga
    12181 cggcacgccg agcacgacca gaccgcgatc ccggtatttc cgccacagcg cttcgagatc
    12241 tttatattgc ggcgtcaacc cgcattggga cgccgtgttc acgatcaaaa ctggatggcc
    12301 ggcgaattgg gacagcttca gcggcgtgcc gtcgatggcg gtgaattcaa aatcatacgc
    12361 cgacatggca aaccctctca tacatccaga ttgacgacgt tgagcgcgtt gtcctgaatg
    12421 aattcgcgcc gcggctcgac cacatcgccc atcagtttgg cgaacagatc gtcggccgcg
    12481 tccgcgtgct cgaccctcac ctgcagcagt gtgcgcgcat tctcgtccag cgtggtttcc
    12541 cagagctggt tcgggttcat ctcacccaag cctttgtagc gctgcaacgc gagcccgtga
    12601 cggccccatc ccaggatcgc ttcgagcaat tgggtgggcg aacgaacctc gtgaagctga
    12661 tccttgcggg tcaaacggct ggctttcaaa taggtcgtct gcaattccac ggccatgcgg
    12721 tcgagcttgc gggcgtcggc gcttgcgatc agcgcgccgt cgatcgcgat cgtctctgtc
    12781 accccgcgca cttcgcggcg gaatttcagc cccccatccg gagtcggctc tcccttccag
    12841 ccgcgctctg tttcctccga catggagtcg aggcggcgtg cgatatagct ggcggcttct
    12901 tccgccttct cggcatcgga cagcacttcg ggattgagcg cgcccgcaat cgccgcctgc
    12961 tccagaacga agcgcggata atggcgcggg aatccatcga gcgccgtgcg cgcggcgcgg
    13021 gcgcgctcca acagatgcgc cagatcggcg ccgccaatgc gttcgccccc ggacagcgtg
    13081 agcgcgctgt ccttgatgcc ctcttcgatc agatggtttt ccagctcgcg ctcatccttg
    13141 agatagcgct cggatttgcc gcgcgcggct ttgtagagcg gcggttgggc gatgaagaga
    13201 tggccgcgct cgatcagctc gggcatctgc cggtagaaaa atgtcagcag cagcgtgcgg
    13261 atatgggcgc cgtccacatc ggcgtcggtc atgatgatga tcttatggta gcgcagtttg
    13321 tcggggttga actcctcgcg cccgataccg gtgccgagcg cggtgatgag agtggcgatt
    13381 tcctgcgagc ccagcatctt gtcgaaacgc gcgcgctcca cgttcaggat cttgccgcgc
    13441 aagggcaaca cggcctgatt ggcgcgattg cgcccctgct tggccgagcc gccggcggaa
    13501 tcgccttcga cgatgaagat ctcgcatttg gccggatcgc gctcctggca atcggcgagc
    13561 ttgccgggca gccctacgcc gtcgagcgcc cctttgcgcc gtgtcagctc gcgcgccttg
    13621 cgggccgcct cgcgcgcggt ggccgcctcg atcaccttgc ccacgatgcc gcgtgcttcg
    13681 gcgggatgtt cctcgaacca acggccgagc tgttccagca ccgcgccttc cacggcgggt
    13741 cgcacttcgg acgacactag cttgtctttt gtctgggagg agaatttcgg gtccggtact
    13801 ttgatcgaca gcacgcaggt caaaccctcg cggcaatcat cgccggtcag cggcacgcga
    13861 tcctttttgc cgatgccgga ctcatcggca tatttcgtca ccacgcgcgt gagcgccgcg
    13921 cggaagcccg cgagatgggt gccaccgtcg cgctggggga tattgttggt aaagcacagc
    13981 atgttctcgt gataggaatc gttccacgaa agcgccgctt ccacccgcat gttgtcgcgg
    14041 tccgccccga tcaaaatcgg attggggatg agcccggttt tggcgcggtc gagatagcgc
    14101 acgaacgctt ccagcccgcc ttcatagtgg agcaggacct ctttcttttc gacgccgcgc
    14161 ttgtcgatga gccggatcgt gacaccggaa tttaggaagg cgagttcgcg caaccggtgc
    14221 tccagcgtcg cgaaatcgaa ctcggtcttg gtgaaggtct tggtgctggg gaggaatgtg
    14281 acctcggtgc cacgacgtcc attcgccttg ccgacgattg tcagcggcgc caccggatcg
    14341 ccgtcgcgaa agcgcatgta atgctcatcg ccgtcgcgcc agatgcgcaa atccagccat
    14401 tcggacagcg cgttcacaac cgacacgccg acgccatgca gcccacccga aaccttgtag
    14461 gcgttctgct cgaatttccc gcccgcatgg agctgggtca tgatgacttc ggccgccgac
    14521 acgccttctt ccttatggat gtcgaccggc acgccgcgcc cattgtcgcg cactgtggcg
    14581 gacccgtcgg gattcagctc cgcctcgatg cgatcggcat agccggcaag cgtctcgtcg
    14641 atggcgttgt cgatgacctc atagaccatg tgatgcaggc ccgaaccgtc atcggtatcc
    14701 ccgatataca tgcccggccg tttgcgcacg gcatccagac ctttgaggac cttgatcgag
    14761 tccgcgtcat aggattgcgc gctgctgccg ttgccgttgg gattgggtcg attgtcttgt
    14821 tctgccatca gcgctccatg ggtacgaaac ggccggcgtc gatggaaaag aaggccgcat
    14881 gctcctgcag cggtgcgaac aggcttctgt cggttcccgt gagccaggcc tggcttccca
    14941 gcgcgaggat ttccgcgaac aaggctgcgc ggcgctggac gtccagatgg gccgcgattt
    15001 cgtccagcag cagcaagggc gcgatgccgt cacggcgcct cttctggatc caggcattgg
    15061 cgagcacgat ggagatgagc agcgcctttt gctcgccggt ggagcaatcg cgcgcgtccg
    15121 agcgcttggc cgtgtggcga acctccagat cggcaaggtg ggggccagcg tttgtgcgcc
    15181 cagcctcggc gtcgcgttcg cgtgacgccg cgagagcgct ttgcagacgc gccggatcgt
    15241 tggcgcatcc ttgcaacgaa tcctgaaggg acagatgcgc acagggaaag gcgccttcca
    15301 tcccgcggat ttcgagttcg ccattcaatc tttcgatgac cgagagccgc gccgccgtca
    15361 ggcccgcacc ctccgtggcc atggtttctt ccagcccgtc cagccagacg cgatcgcgcg
    15421 atccatcgcg caagaggcgc agccgctcat gcatcgcccg ctcatagcgc gtggcgcgct
    15481 tggcatgcgt ggaatcgagc gcaaagacca aacggtcgag aaaacggcgg cgttcggatg
    15541 cgccttccag gaatagccgg tccatcgcag gcgtgagcca cagcatcgga atgagttcg
//
LOCUS       DASYEG010000134        10121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_913090, whole genome shotgun sequence.
ACCESSION   DASYEG010000134 DASYEG010000000
VERSION     DASYEG010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 10121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10121
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913090"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VGM72_05855"
     CDS             <1..372
                     /locus_tag="VGM72_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264823.1"
                     /translation="EMEEEYFERTKVWPIMHIIAMKKSILEEHPWAARNLYNAFLESK
                     RRSIERILDPAVSRYPVPWLTTYARKMRDRFGDLFPYGIEENRPTWEQMALYTYQQGI
                     AHRQYKPEEIFPKGIMTKVVI"
     gene            614..1594
                     /locus_tag="VGM72_05860"
     CDS             614..1594
                     /locus_tag="VGM72_05860"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY4264824.1"
                     /translation="MLTLADILRVAQDRLAAGDGQAAADIYRQILDREPTNTEALLGA
                     LRAAMAIGNPSAIEAAQHRLEQVYRTAGAMTQLAVAQAQLKDALRALEEASVSAQRAL
                     GADPSLPEANTIAWLIGHHLGNFPQFFSQFGQDRYLYQHLFENIRDGVFVDVGAYDGL
                     TGSNTLFFEKFLGWKGVCIEPDAAQFAKLTMFRSCDCVQTCIADKDATARFLSVAGGL
                     TMMGGLVDHYEPGDLKMVTERSGAKIVTVATQRLDSVLSKRNITSIDYLSIDTEGSEL
                     AILRSFDFSRFKVRALSVENNRNAALIPDYMQSLGYRRIARLGVDDIYTK"
     gene            1739..2449
                     /gene="pcaH"
                     /locus_tag="VGM72_05865"
     CDS             1739..2449
                     /gene="pcaH"
                     /locus_tag="VGM72_05865"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HEY4264825.1"
                     /translation="MTGPVPYAREDKAAQPPLAFPAFKASVARAPSQPPIRAPQTLTE
                     ITGPSGEGVWERLMGPALTDLTRQHRGEPVGERIIVSGRVLDENGRPVPNTMIEILQA
                     NAAGRYVHQYDQHDAPLDENFTGAGRVISDAGGNYRFITIKPGAYPWYNQRNAWRPAH
                     IHFSLMGAAFANRLITQMYFPGDPLFGLDAIANAVPEPARERIVARFDPDTTEPFWAL
                     GYRFDFVLRGRDATPMEH"
     gene            2452..3099
                     /gene="pcaG"
                     /locus_tag="VGM72_05870"
     CDS             2452..3099
                     /gene="pcaG"
                     /locus_tag="VGM72_05870"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HEY4264826.1"
                     /translation="MAGQTPWQTTGPFFHFGLPWKGGADLTGDSELGARKDLIQPGHD
                     YGVTATSGMRAHTIGERIEIFGHVWDNKGAPIVDCLIELWQANAAGRYAHPEDTREDV
                     APDPAFIGFGRTASDRQGGYRFRTIKPGRVPGPGNSLQAPHIALGLMGPGFLKRLATR
                     VYFPSASENAEDPILGLVPEERRSTLIAVRAPESPSLYRFDIRLGGAGETVFFDI"
     gene            3133..4482
                     /locus_tag="VGM72_05875"
     CDS             3133..4482
                     /locus_tag="VGM72_05875"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY4264827.1"
                     /translation="MEVTRTLARYVVNSKLGDIPDAVKREGVRSILNWLGCAVGAARH
                     EAVECAMAALLPFAGPAQASILGREEKTDILNAALMNGITSHVFDFDDTHLPTIIHPS
                     GPVASAIFSLAEYRDVTGAELLHAFILGVEVECRIGNAVYPNHYDVGWHITGTTGVFG
                     AAAAAGKLLGLDEQRMLWALGIAGTQSSGFREMFGTHCKSFHPGRAAQNGLAAALLAE
                     KNFTSSNQVLEAKRGFANVMSTKQDYTRITEGLGKTFEVTLNSYKPFACGIVIHPAID
                     GCVQLKKENGLSGTEIEKVELTVAPLVLELTGKRTPQVGLEGKFSVFHSCAVALLKGA
                     AGEKEYSDAMVRDPQVIALRDRVQAVIDPKLRDDEVHVRLTLKDGRVLEKYVEHAIGS
                     KEVPMTNEQLEAKFRGLADDILGPQASANLLALAWSIEKLKDASEICRAASPNRRGA"
     gene            4553..5539
                     /locus_tag="VGM72_05880"
     CDS             4553..5539
                     /locus_tag="VGM72_05880"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264828.1"
                     /translation="MARLVAAFGSSHSVMLAATRSDWSRGFRESDRRMPLFDSNGAAT
                     SYDALLTAAPRDAETRVSAEALSAAYDRTFAAIAELKRQMDAVALDCLIVIGDDQHEL
                     FQDTLMPSLAIYYGKTIRNAARLNVSGADWYKRAQMERLEPERDAHYPVHGDLALHLI
                     TGLTTREFDVCAVREIARDQYEGHAYSYIHRTYLGARELPIVPVMLNTYYPPNPVTPK
                     RCVQLGRELKVLIESLAGDLRVGVIASGGLSHFVVDEELDRGVLTALEKKDLDTLAGL
                     DPKRLQAGSSEIRSWIVTAAAATDLNLVWSDYIPAYRTPALTGTGLGFARWA"
     gene            5551..5943
                     /locus_tag="VGM72_05885"
     CDS             5551..5943
                     /locus_tag="VGM72_05885"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY4264829.1"
                     /translation="MRQKSIEVKGLSHHGAPIPTACRVGPILVTSRLMGKDPETGVMP
                     TDPEAQAVLCFENLKRVLAAGGMDLGDVVKLTVYLVSDSYRTAVNKPWHEHFPDPEHR
                     PTRDSLVMPLRGGNLIQIEAWAVAKELG"
     gene            complement(5962..8238)
                     /locus_tag="VGM72_05890"
     CDS             complement(5962..8238)
                     /locus_tag="VGM72_05890"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4264830.1"
                     /translation="MLEQALALHQKGKIDAAASLYLQIIAQNPDHADALYLLGMIEFQ
                     RRNNAAAIDLMGRALALNPHNAEYLVNYGIALRRSERFHEALINFDRALAIRPDSVEA
                     LISRGNVLRNLNRLSDALASFDRALALRPDFAELFYNRGLVMRDMLRFEDALASFDRA
                     LSLRPAYTDALHGRALVLQGLMRFDDALTGYDQVLVLQPDRVKALADRANLLREQRRF
                     ADALAGYDRALAIKPDYPELLVNRGNVLSDLGRLDEALASYERALGIRADYTGALINR
                     GNVLRDLKRFDDAAASFDRALAIQPDYPFLAGHRLYFKMSICDWQGIEDDIGAIERRI
                     EAQQLAAMPFHLLATPLSAFEQKSCSEIYMRAQNPQVLQALDRTGHQGRNRIRLGYFS
                     TDFRNHVVADHIAGLIAQHDRTKFEVIAFSFGPNTRDSTRARLEKSFDRFIEAGAFSD
                     RDVALLARKFEIDIAIDLNGFTQGSRPRVFAMRTAPIQVNYFGYPGTTGSEHIDYIIA
                     DPVVIPEDQRRYYREKIAYLPDTYQINDSQRLISSGPIDRAASGLPEGAFVFCCFNNN
                     FKIAPEIFDVWMRVLKAVGGSVLWLLQDNAAAAKNLRAHAHARGVDPKRIMFAPRAGL
                     AEYLGRHRLADLFLDTLPYNAHATASAALWTGLPVLTCLGKTFPGRVGASLLNAIGLP
                     DLVAKDLGEYEAMAVDLAANPQKLLALRQTLAVNRTTHPLFDTARCTRAIEQAYLSMW
                     ERHQAGLAPDHIHVRPEV"
     gene            8416..9255
                     /locus_tag="VGM72_05895"
     CDS             8416..9255
                     /locus_tag="VGM72_05895"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEY4264831.1"
                     /translation="MDSKNPAITIPQLHQMKRDGKKIVGVVAWDYQIAKIIDRAGVDI
                     ISVGDTVGINLWGHATPLEVTMDEMVVVCKAVRRGTSRALVSCDFPFGPLQEGTESAV
                     RAAIRLVKEGGADIIKLDGAADFPEAVRAISRAGIPVFAQFGLTPQTALALGMQYNAM
                     NKPGAELAADMAAKMVDDAKRLEDSGATLLDFTNSGPVAGEAVVRAVSIPVIGGFGGG
                     PWLDGRMRMAHAAIGYAAAALESKTENYANVAKTTLDAISSYAADVRAARQIKGAAPA
                     KLP"
     gene            complement(9273..9608)
                     /locus_tag="VGM72_05900"
     CDS             complement(9273..9608)
                     /locus_tag="VGM72_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264832.1"
                     /translation="MMNKTLLTLAFVGTIGVTGSALAQNYGSAGTPAAANAEPDRVVV
                     AERVVSENPTVIERQVIVGKIMRMPERNTGVSKIGGSVGGAIGGYYINGSTAAQNRPL
                     DIPPDAYTR"
     gene            9864..>10121
                     /locus_tag="VGM72_05905"
     CDS             9864..>10121
                     /locus_tag="VGM72_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4264833.1"
                     /translation="MPTAIIDGITTRYEVVGSGPPLLMYAPAGFDASLEKWSTQGVYA
                     RIKLLDHLSKTYSCILFDRRECGQSGGRVERVTWAHYVAQGK"
BASE COUNT         2057 a         3072 c         3004 g         1988 t
ORIGIN      
        1 gaaatggagg aggaatattt cgaacgcacc aaggtgtggc cgatcatgca catcatcgcg
       61 atgaagaaat ccatcctcga ggagcatccc tgggccgcac gcaatctcta caatgcgttc
      121 ctggaatcca aacgccgcag catcgagcgc attctcgacc cggccgtgtc acgctatccg
      181 gtgccatggc tgacgaccta tgcgcggaaa atgcgcgatc gttttggcga tcttttcccc
      241 tatggcatcg aagaaaatcg cccgacctgg gaacagatgg cgctctatac ctatcagcag
      301 ggtatcgccc accggcaata caaaccggag gaaattttcc ctaagggcat catgacgaaa
      361 gtggtgatct gaacgcttat ggaaggtgcg gctgcgcagg cgcggctcgc accttctgta
      421 actccggcat ttgtaatact ctgagccgcg ttaatgtagg gcaatatcca atgccgaatt
      481 acatttttag cgccggcaca agaaaaatct gtgcgttcaa tagcttgtgt ttgaatttgt
      541 gctatcagcg ttgatgcgtc ctgaaccgca cagtcaagac ggaaatgggc gtaacattac
      601 gcgcgatgac accgtgctca cgcttgccga catacttcgg gtagcccagg accgtttggc
      661 ggccggcgat ggccaggcgg cggcggatat ttatcggcaa attctcgacc gggagcccac
      721 caataccgaa gcgcttctgg gcgcactccg cgcggcgatg gcaatcggca atccttccgc
      781 gatcgaggcg gcccagcatc ggctggagca ggtctaccgt acggccggag caatgaccca
      841 gcttgccgtc gcccaggcgc aattgaagga tgcgttgcgc gcgcttgagg aggcttccgt
      901 ttccgcacaa cgcgcgcttg gcgctgatcc ttcgctgccc gaggcgaata caattgcctg
      961 gctcatcggc catcatttgg ggaattttcc gcaattcttt tcgcaattcg gccaggaccg
     1021 ctatctgtat cagcatctgt tcgagaacat tcgcgatggt gtgttcgtcg atgtcggcgc
     1081 ctatgacggg ttgacgggca gcaacacgct gttctttgag aaatttctcg gttggaaagg
     1141 cgtgtgcatc gagcccgacg ctgcgcagtt cgccaagctg acgatgttcc ggtcatgcga
     1201 ctgcgtgcaa acctgcatcg cggacaagga cgcgacggcg cgattcctga gtgtggcggg
     1261 tgggctgacc atgatgggcg gcctggtcga tcattacgaa cccggcgatc tcaagatggt
     1321 cacggaacgc tcaggcgcaa agatcgtcac cgtagccacg caacgcctcg actcggtgct
     1381 gagcaaacgc aacatcacgt caatcgatta tctgtcgatc gacacagagg gttcggagct
     1441 tgccattctg cgttccttcg atttttcccg attcaaggtt cgcgccctgt cggtcgagaa
     1501 caatcgcaac gccgcgctga tcccggacta tatgcagagc ctgggctacc gccgcatcgc
     1561 ccggctcggt gtcgacgaca tctatacgaa atgagccggc caggcgtggg ttaacgcctg
     1621 caatccgtcc ccgtatcaca aagctaaccc tgcgtggctc cgcggagctt gcggatcgct
     1681 tgcatcggtg aaccgcattc tgtatcgcgt tcgtgacttg aatcggcggg agacgagcat
     1741 gaccggcccg gtgccctatg cgcgcgaaga caaagcggcc cagccgccgc tcgcttttcc
     1801 cgcgttcaaa gcttcggtcg cgcgcgcgcc cagccagccg ccgatccgcg cgccgcagac
     1861 cttgaccgag atcaccggtc cgtccggcga aggcgtgtgg gaaagactga tgggccccgc
     1921 gctgaccgac ctcacccgcc agcaccgggg tgaacccgtg ggcgaacgca tcatcgtctc
     1981 gggccgcgtg cttgacgaga acggccgtcc cgttcccaac acgatgatcg aaatccttca
     2041 ggccaatgcg gcggggcgct atgttcatca atatgatcag catgacgcgc cgctcgatga
     2101 gaatttcacc ggggcgggac gcgtcatcag cgatgccggc ggcaattacc gatttatcac
     2161 catcaagccg ggtgcctatc cctggtacaa tcagcgcaac gcatggcggc ccgcgcacat
     2221 ccatttctca ctgatgggtg ctgcgttcgc gaaccggctc atcacccaga tgtatttccc
     2281 gggcgatccc ttgtttggac tcgacgccat cgccaatgcg gtccccgaac ccgcgcgcga
     2341 gcgcatcgtg gcgcggttcg atccggacac caccgaaccc ttttgggcgc tcggttatcg
     2401 ctttgacttc gtactgcgcg gtcgcgacgc cacgcccatg gagcattgag catggcgggg
     2461 cagactccct ggcagaccac cggaccattt ttccatttcg ggttgccctg gaagggcggc
     2521 gccgatttga ccggcgattc cgagctcggt gcgcggaaag atctgatcca gccggggcat
     2581 gattatggcg ttaccgcaac gtccgggatg cgcgcgcata cgatcggcga acgcatcgag
     2641 attttcggcc atgtctggga caacaagggt gcgcccatcg tcgattgcct gatcgagctc
     2701 tggcaggcga atgcggcggg ccgctatgcg catcccgaag acacgcgcga ggatgtcgcg
     2761 cccgatccgg cgttcatcgg gtttggccgg acggcatcgg atcggcaagg cggctaccgc
     2821 ttccggacca tcaaaccggg gcgcgtgccc ggacccggca atagtctaca ggcgccgcat
     2881 atcgcgctgg gcttgatggg gcctggcttt ttgaagcgcc tcgccacgcg ggtgtatttt
     2941 ccgtcggcat ccgaaaatgc cgaggatccg attctcggtc tcgtacccga agagcgccgg
     3001 tcgacattga tcgcggtccg tgcgccggaa agcccgagtc tctatcgttt cgatatccgt
     3061 cttggcggtg ccggcgaaac ggtcttcttt gacatctgag cgcggttttg agcgcgaact
     3121 ttggagtgag ctatggaagt gacacgcaca ttggcgcgct atgtggtgaa ttcgaagcta
     3181 ggtgatattc ccgacgcggt gaagcgcgag ggcgttcgct ccatcctcaa ttggctcggt
     3241 tgcgcggtgg gcgcggcgcg tcatgaggcg gtcgagtgcg cgatggcggc acttttgccg
     3301 ttcgcaggcc ccgcgcaggc ctccattctg gggcgcgaag aaaagaccga catcctcaat
     3361 gccgccctga tgaacggcat caccagccat gtgttcgatt tcgacgacac gcatctgccc
     3421 accatcatcc atccgtccgg accggtcgcg tcggcgattt tttcgcttgc ggaataccgg
     3481 gatgtcacgg gcgccgaatt gctccatgcg ttcatcctcg gcgtcgaggt cgaatgccgc
     3541 atcggcaacg ccgtctatcc caatcattac gatgtcggct ggcacatcac gggcaccacc
     3601 ggggttttcg gcgccgccgc cgcggccggc aaattgctgg gcctcgacga acagcggatg
     3661 ctgtgggcgc tcggcatcgc gggcacgcaa tcttccggct ttcgcgagat gttcggcacc
     3721 cattgcaaga gcttccaccc cggacgcgcc gcccagaatg gcctcgccgc cgcgctgctg
     3781 gcagaaaaga acttcaccag ctcgaaccag gtactcgaag ccaagcgcgg cttcgccaat
     3841 gtcatgtcga cgaaacagga ttacacccgc atcaccgagg ggctcggtaa aaccttcgag
     3901 gtgacgctga actcctacaa gccatttgcc tgcggcatcg ttattcatcc tgccatcgat
     3961 ggttgcgtgc aactgaagaa ggaaaacggc ctttcaggga ccgagatcga aaaagtcgag
     4021 ctcaccgtgg cgcccctggt gctggaactc accggcaagc gcacgcctca ggtgggcctc
     4081 gaaggcaaat tcagcgtgtt ccattcctgc gcggtggcgc ttctgaaggg ggcggcgggc
     4141 gagaaagaat actccgatgc catggtacgc gatccccagg tcatcgccct tcgcgaccgc
     4201 gtgcaggccg tcatcgatcc caagctgcgc gacgacgaag tccatgtgcg gctcacactc
     4261 aaggacggcc gcgttctcga aaaatatgtc gagcacgcca tcggcagcaa agaagtcccg
     4321 atgactaacg aacagctcga agcgaaattc cgcggattgg ccgacgacat tctggggccg
     4381 caagcctccg cgaatctgct cgcgctcgcg tggagcatcg agaagctcaa ggacgcatcg
     4441 gaaatctgcc gggcggcatc gcccaaccgc agaggcgcct aggttagaag cgcataggaa
     4501 gcggccggcg cttcctctaa catgtcggaa tagcgaacgt tccggagaga atatggcgcg
     4561 gcttgttgcg gctttcggct cgtcccacag cgtgatgctg gcggcgacgc gcagcgattg
     4621 gagccgcggt ttccgcgaat ccgaccgccg catgccgctg ttcgattcga acggcgctgc
     4681 caccagctat gatgcgcttc tcaccgcggc accgcgcgat gcggaaaccc gtgtgagcgc
     4741 cgaggcgttg agcgccgcct atgaccggac gttcgccgca attgccgagc tgaaacgtca
     4801 gatggatgcg gtcgcgctgg attgcctcat cgtcatcggc gacgaccagc acgagctgtt
     4861 tcaggatact ctgatgccgt ctcttgccat ctattacggc aagaccatcc gcaacgccgc
     4921 acgcttgaat gtgtccggcg cggattggta caaacgcgct cagatggagc ggctggaacc
     4981 ggagcgcgat gcccattatc cggtccatgg ggacctggcg ctgcatctca tcacgggtct
     5041 caccacgcgt gaattcgatg tttgcgcggt acgcgagatc gcccgcgatc aatatgaggg
     5101 ccacgcctat tcctatatcc atcgcaccta tctgggcgca cgcgagcttc cgatcgtgcc
     5161 ggtcatgttg aacacatatt atccgcccaa tccggtgacg ccgaagcgct gcgtgcaatt
     5221 gggcagagag ttgaaggtcc tgatcgagtc gctcgcaggt gacctgcgcg tgggcgtgat
     5281 cgccagcggc ggcctttccc atttcgtcgt cgatgaagag ttggatcgcg gcgtgctcac
     5341 ggcgcttgag aaaaaagacc tcgacactct cgctggtctc gatcccaaaa ggctacaagc
     5401 cggaagctcc gagatcagaa gctggatcgt cacggcagcc gccgccaccg atctcaatct
     5461 cgtctggagc gattatattc ctgcctatcg tacccctgcc ctgaccggca cggggctcgg
     5521 tttcgcgcgc tgggcataaa tggagaaccc atgagacaga aaagcatcga agtgaaaggg
     5581 ctttcccatc atggggcgcc cattcccacc gcctgccgcg tggggccgat cctcgtcacc
     5641 tcgcggctga tgggcaagga cccggagacg ggcgtcatgc ccaccgaccc ggaagcgcag
     5701 gccgtgctgt gctttgagaa tctcaagcgc gtgctggccg ccggcggcat ggatcttggc
     5761 gacgtggtga agctaaccgt ctatctcgtc agcgattcct atcgcactgc cgtcaacaag
     5821 ccatggcacg agcattttcc cgatcccgaa catcggccga cccgcgattc gctggtcatg
     5881 ccgctcagag gcggcaatct gatccagatc gaagcctggg cggtcgccaa ggaattaggc
     5941 taggacgctt ctccgaaacg atcaaacctc cggccgcaca tggatgtggt cgggagcaag
     6001 ccccgcctga tgccgctccc acatcgacaa ataggcctgt tcgatggccc tcgtgcatcg
     6061 cgccgtatcg aagagcggat gcgtcgtacg attgaccgcg agggtttggc gcaacgccag
     6121 caatttctgc ggattggccg cgaggtcaac agccatggcc tcatattcgc cgaggtcttt
     6181 cgcgacaaga tccggcagac caatagcatt gagaagactc gcgccaacgc gtcctggaaa
     6241 tgttttgccc aggcatgtca gcaccggcag ccctgtccac agagcagcac ttgccgtggc
     6301 atgcgcgttg taaggaagcg tgtccagaaa cagatcggcc aatcgatggc gccccaaata
     6361 ttcggccaga ccggcgcgcg gagcaaacat gattcgcttc gggtccacgc cgcgcgcgtg
     6421 tgcgtgtgct ctcaggtttt tcgcggctgc cgcgttgtct tgcaatagcc agaggacgct
     6481 tccgccgaca gccttcaaca cacgcatcca gacgtcgaag atttccggcg cgatcttgaa
     6541 attgttgttg aagcaacaga agacgaatgc tccctccggc agaccactgg ccgccctgtc
     6601 gatcggtccg ctggagatta agcgttgaga atcgttgatc tgatacgtat ccggcaaata
     6661 ggcgattttc tcccgataat atcgccgttg gtcttcgggg atcacaaccg ggtcggcaat
     6721 gatatagtcg atatgctcgc tccccgtggt tcctggatag ccgaaatagt tgacctgaat
     6781 gggcgccgtc ctcatggcga acacacgagg cctgctgcct tgcgtaaaac cgttgaggtc
     6841 gatggcgatg tcgatttcaa acttgcgcgc cagcaacgcg acatcccgat ccgagaaagc
     6901 gcccgcctcg ataaagcggt cgaacgattt ctccaatcgc gctctcgtcg aatctcgcgt
     6961 attcgggcca aaagaaaaag cgatgacttc gaatttcgtc cggtcatgtt gggcaatcaa
     7021 tccggcgata tgatcggcca cgacgtggtt tctgaaatcc gtcgaaaaat atccgagccg
     7081 gatccggttg cggccttgat gtcccgtgcg atcgagtgct tgcaaaactt gagggttttg
     7141 cgcccgcata tagatttccg aacaggattt ctgttcgaat gcggataaag gcgtcgcgag
     7201 aagatggaac ggcatcgcgg ccaattgctg cgcctcgatc ctgcgctcaa tcgccccgat
     7261 atcatcctcg atcccctgcc agtcgcagat gctcattttg aaatacagcc gatgtccggc
     7321 caggaacggg taatcgggct gaatcgccag ggcgcggtcg aaactcgcgg ctgcatcgtc
     7381 gaaacgcttc aaatccctaa gcacattgcc ccggtttatg agggctccgg tgtaatccgc
     7441 tcttatgccg agtgcgcgtt catagctggc gagcgcctca tccaagcgac ccaaatctga
     7501 aagcacattg ccacgattga cgagcagctc cggataatcc ggcttgatgg ccagagcgcg
     7561 atcatagccg gcgagcgcat cggcaaaacg gcgttgttcg cgaagcagat tggctctgtc
     7621 cgccagcgcc ttcacccggt ccggctgaag taccagcacc tgatcgtagc cggtgagcgc
     7681 gtcgtcgaac cgcatgaggc cctgaagaac aagtgcgcga ccatgcagcg catcggtata
     7741 agcgggcctg agagacagcg cgcgatcgaa acttgccagt gcgtcttcga aacgcagcat
     7801 atcccgcatc acgagaccgc gattgtagaa aagctccgca aaatccggcc ttaatgcaag
     7861 cgcacggtcg aaactggcga gcgcatcgga caagcgattg aggtttcgca gaacattgcc
     7921 gcggctgatg agcgcttcca cggaatcggg ccggatcgcc agcgcgcggt cgaaatttat
     7981 cagcgcttca tgaaagcgct cggaccggcg aagggcgatc ccataattga cgaggtattc
     8041 cgcgttatgg ggattgagcg ccagcgctcg gcccatcaga tcgatcgccg ccgcattgtt
     8101 ccttcgttgg aattcgatca tgcccaacag gtatagggcg tccgcgtgat ccgggttttg
     8161 cgcaatgatc tggagataga gagacgccgc cgcatcgatc ttgccttttt gatgaagggc
     8221 aagggcttgt tcaagcatcg aggaaaccgg tcttgcgcgc gcggctcgca cgatatcggc
     8281 ggagcgtggt gttcaccagt taaagtttgc gttgcatttc cgatgcggct tcttacctgg
     8341 gtcggcattg acgaaaaatg cgctacggtg ctacggcccg gcaacagccg ccgtcctcaa
     8401 cgaaagatcg ccgtcatgga ttccaagaac cccgccatca ccattcccca actccaccag
     8461 atgaagcgcg acgggaaaaa aatcgtcggc gtcgtcgcct gggattatca gatcgccaag
     8521 atcattgacc gcgcgggtgt cgacatcatc tccgtgggcg acacggtggg catcaatctc
     8581 tggggccatg cgacgccgct cgaagtcacc atggatgaga tggtggtggt ctgcaaagcg
     8641 gtgcggcgcg ggaccagccg tgcgcttgtc tcctgcgact ttcctttcgg tcccctgcag
     8701 gaagggaccg aaagcgccgt gcgggccgca atccgcctgg taaaggaagg cggcgccgac
     8761 atcatcaagc tcgacggcgc ggccgatttt cccgaagccg tgcgtgcgat ctctcgcgcc
     8821 ggcattcccg tcttcgcgca attcggattg acgccgcaga ccgcccttgc tctgggcatg
     8881 caatacaatg ccatgaacaa accgggcgca gaactcgcag ccgatatggc ggccaagatg
     8941 gtagacgacg ccaagcgtct ggaagattcg ggcgccacgc ttctggattt caccaattcg
     9001 ggtcccgtcg ccggggaagc cgtcgtgcgc gcagtttcca ttccggtcat cggcggcttc
     9061 ggcggcggcc cctggctcga tggacggatg cgtatggcgc atgcggccat cggctatgcg
     9121 gcggccgcgc tggaatcaaa aacggaaaat tatgccaacg tcgccaaaac tacgctcgac
     9181 gcgatctcgt cctatgcggc cgatgtccgc gcggcccgcc aaatcaaggg agcagctccc
     9241 gccaagctcc cctgaccgga tggcaaatga actcagcgcg tgtaagcgtc cgggggaatg
     9301 tccaaggggc ggttttgggc ggccgttgaa ccgttgatgt aatatccgcc gatggcgcca
     9361 ccgacactgc cgcctatctt gctgacgccg gtgttgcgtt ccggcatgcg catgatcttg
     9421 ccgacaatga cctgacgctc gatcaccgtg ggattttcgc tgacgacgcg ctccgcgacg
     9481 acgacacgat ccggctctgc gttcgccgca gccggcgtac cggcgctgcc gtaattctgc
     9541 gcaagggcag aaccggtcac gccgatcgtc cccacaaaag ccagggtcaa aagagttttg
     9601 ttcatcatgg gtcgtctact ccgcctgtac cgggccccat ccggcttctg aaaggtccga
     9661 aaaatcggac atccgatatg acaacggcgc tcgacgaact tctttgcacg catatgcgaa
     9721 aaaaagacgc accatggccg ggccaacgct tcaatggggc tcagccacat cgcccctttg
     9781 ttgaaccccg ccgcaactgt ggcttgacag gcggacaatt tcgtctgcga ttgcgcaaac
     9841 tccttggact gtgaggaccg gccatgccca ccgccatcat cgacggcatc accacgcgct
     9901 atgaagtggt cggctcgggg ccgccgctgc tgatgtatgc gcccgccgga ttcgacgcga
     9961 gcctggaaaa atggtcgacg caaggcgttt atgccaggat caaattgctc gatcatctgt
    10021 cgaagacata tagctgcatt cttttcgacc ggcgcgaatg cggccaatcg ggagggcggg
    10081 tggagcgcgt gacatgggcg cattacgtgg cgcaaggcaa a
//
LOCUS       DASYEG010000135        20448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3231860, whole genome shotgun sequence.
ACCESSION   DASYEG010000135 DASYEG010000000
VERSION     DASYEG010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 20448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20448
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3231860"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VGM72_05910"
     CDS             complement(<1..329)
                     /locus_tag="VGM72_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HEY4264834.1"
                     /translation="MANNPGTQSAAKTAQTTEAAGEANLLDQVIGATRQTDREQAQML
                     LKALTEEALKGTVTYSRNMTLTFRKAIELIDKQLSQQVAAIMHHPDFLKLEGSWRGLN
                     YLVMNSET"
     gene            complement(329..841)
                     /gene="tssB"
                     /locus_tag="VGM72_05915"
     CDS             complement(329..841)
                     /gene="tssB"
                     /locus_tag="VGM72_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HEY4264835.1"
                     /translation="MASINDKLERVRKPRVHIKYEIEEEGAVVERELPFVVGVMGDFS
                     GNPSKPLPPLKDRKFVQIDRDNFNDVMARISPELNIRVNNTMKDDGSEMAVQLKFRSM
                     DDFEPGKIAESVPALKKLLETRNQLRDLATKADLSPELEGLLERVLQNEDDLKKLSEQ
                     LGLGRSGEGE"
     gene            complement(892..2016)
                     /gene="tssA"
                     /locus_tag="VGM72_05920"
     CDS             complement(892..2016)
                     /gene="tssA"
                     /locus_tag="VGM72_05920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HEY4264836.1"
                     /translation="MGSPPCIDIEQLLAPISEGAPAGANIREDVVPTSVYFRLKDARS
                     AARSAEKRADAEGEYLGVTPEWRTIQDLATTVLSERSKDLEVTAWLIEALVRLQGFPG
                     LRDGCRLARGLVEQYWDTIHSLEDEQGVATKVAPLTGLNGADGTGTLLQPIRKVSLTK
                     AVGEGPFAAYHYEQAWALSEIADPEVRARREQSGVVTLDRFTAVANASGGVFYIALID
                     DIQASIEELNRLGAALDERAGDASPPTSAIREVLSAILETVQRFSKDLVARATQLSQR
                     SEGGGEVVPLANGSAAEPGGYNLNGGIHSREDALRVLLHVAQYFHANEPHSPIAISLE
                     ETVRRARLSFSDLLMELLPDRSAWRSALTSAGIKPPPEGS"
     gene            2267..3001
                     /locus_tag="VGM72_05925"
     CDS             2267..3001
                     /locus_tag="VGM72_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264837.1"
                     /translation="MSITAEPGESRMSSKAIVLKRNNSDAAKLKALQQLAPQFLVEYS
                     SGGQLAALTNNFGTLDYLLVTGGHGDFVNKKPSKFNDADIVSAKKWISNVKGTFKAII
                     LDTCFSSALAGSFLPFLPNGGCLVCAHGTAEGWADGFVTGNNTRTIGELLADVIDNFA
                     GLGSFASVSLAIKKPINQVLYTGNGGADRKVGLDARSNMGMEVDTELELKEVDIYLAR
                     DRIDVVATKSDALKKLLKDNITTTIV"
     gene            3162..5822
                     /gene="tssH"
                     /locus_tag="VGM72_05930"
     CDS             3162..5822
                     /gene="tssH"
                     /locus_tag="VGM72_05930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HEY4264838.1"
                     /translation="MAAIDLKSLMGKLNEPCRRALEAAAGLTLSRTHYNVEVEHWLLK
                     LLDVRGGDLVACLKHYEVDEARLAADLNKVLDRLKTGNSRAPALSPQIVSLCREAWLT
                     ASLVHGAARVRSGHLLLALLSDDMLAQLAREASGQFNKIPIDSLRADFSKIIADTVEA
                     EAGASATSGAQGSSGQSPAAAAGGALDQYTIDLTARAKAGKIDPVLGRDSEIRQVIDI
                     LTRRRQNNPILTGEAGVGKTAVVEGFALKIAAGEVPDAMKNVALRTLDLGLLQAGAGV
                     KGEFENRLKSVIDEVKASPTPIILFIDEAHTLIGAGGAAGQGDAANLLKPALARGDLR
                     TIAATTWGEYKKYFERDPALARRFQVVKVEEPDEAPAIAMMRGLVGMLEQHHAVRVLD
                     EAVIEAVRLSKRYIPGRQLPDKSVSVLDTACARVAMSQNAVPAAIEDCRRRIQLISTE
                     MEILGRETASGADHAATLEQLSAGKKSAEDELRHLDIRWADENRLVGILRELRDKLEA
                     AHMGKDGGENLSSAGSMALRDEFERTKQKLVAVQGEEPLVHVSVDAQAVAEVIANWTG
                     IPVGRMVSNEIKTVLSLKETMSERIIGQPHALEAIAQAMRTARAKLTDPRKPIGVFLM
                     VGTSGVGKTETAITLAELLYGGSQSLTVINMSEFKEEHKVSMLLGSPPGYVGFGEGGV
                     LTEAVRRRPYSVVLLDEMEKAHPGVQDVFYQVFDKGQLRDGEGRDIDFKNTVVIMTSN
                     AGTETIAKLCADPETMPDENGLAEALRPELLKVFKPAFLGRVTLVPYFPLSPETLRLI
                     VKLQLDRIQKRIAENYRAKFVYAQDAIEAIAARCTEAESGARNIENVISRGLLPEISA
                     RFLEKMANGESFRQVHLSMDTGGGFKYTID"
     gene            5992..6471
                     /locus_tag="VGM72_05935"
     CDS             5992..6471
                     /locus_tag="VGM72_05935"
                     /inference="COORDINATES: protein motif:HMM:NF017454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HEY4264839.1"
                     /translation="MPIYMKIDSVNGEVTAAGYEKWIDVGSLQFGIGRAISTPVGGGS
                     KREAGHPSISEITLTKSFDNSSIELFSWSTAGKSKTVKIDMVQTGDKDKVSAYLQFEL
                     SDTLISGYSVSSGGDKPSESISLNFTKITEKYTPYGKDNAVGQPILKGYDLVTAKTV"
     gene            6617..8431
                     /gene="tssI"
                     /locus_tag="VGM72_05940"
     CDS             6617..8431
                     /gene="tssI"
                     /locus_tag="VGM72_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HEY4264840.1"
                     /translation="MAGLEDIELDQNERLISVESFHLGKKKILITAFSGTERLSALSR
                     FQLEIMTIGRALDPGEILGKPITLALRIKDQVRKFSGIAGRFQTVQTSIRDYNLHIVE
                     LVPPAWLLSLNQRCKIFPDKKATDVVSQVLKAGGVNVKEKTAGTSREYIVQYCESDFN
                     FISRLLEDEGLFFYFDHGDPNCPIVLGNGSADYSRAGLGALEFTGCIQEWEPQYQVGA
                     NAFEHADWDFKAVKTINGTSKGLAKVQAKGIADRSVYEYPGRYGTADEATAWARARME
                     EHEAGAVKIRGTTADIRVQPASKFKTKDYKLQLPGSTDATDSYVFVSVEHRARDGSGV
                     PFEAPTSYENNFECIPADLNFRPARVSHRPAIAGPQTALVTDGPDEYGRAKVKFPWEQ
                     QGQSRWTRVAQNWAFNQMGTQFLPRINSEVVVEFLDGDPDQPLIVGMVYNGKNKLPFA
                     VPANKTQSGIRGSNWDAAGTANKSNELRFEDKSGSEEIFVHAQKDFKRVVVNDDSLTV
                     EQGNRTLETKTGNVTETLSKGNHSLKVDAGTSKVEAMQSITLKVGSNSVVIDQQGITI
                     KGIMIKIEGSAMANMKSPMTEVDGDGMLTLKGGITMIN"
     gene            8441..9031
                     /locus_tag="VGM72_05945"
     CDS             8441..9031
                     /locus_tag="VGM72_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264841.1"
                     /translation="MEIGALTKVTARQADEVLQLALAAGQSVLQVPGMAPPVYLQHLI
                     SKPAFNEAVQFLAFALPKREAVWWACLCARADLHESAPAPVVAALTAAEAWVYRPTEE
                     NRHAAMERAKEAKFETPAAWAAVAAFWSGPSMAPPDLPAVPPAPHLTGVAVFGAVTLA
                     AVQTEAALADEKRQRYIKIAVDIANGGSGRVDVKGR"
     gene            9042..9332
                     /locus_tag="VGM72_05950"
     CDS             9042..9332
                     /locus_tag="VGM72_05950"
                     /inference="COORDINATES: protein motif:HMM:NF017315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEY4264842.1"
                     /translation="MGMPAARLTDMHVCPMVTGIIPHVGGPVIMGSPTVITGKMPQAR
                     VSDPLTCVGPPDTIVKGSATVLVNSLPAARIGDTTAHGGTIILGFPTVLIGG"
     gene            9346..10524
                     /gene="tagH"
                     /locus_tag="VGM72_05955"
     CDS             9346..10524
                     /gene="tagH"
                     /locus_tag="VGM72_05955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HEY4264843.1"
                     /translation="MNLILTIVQGPPGGAAATRSFDDAALGKRGITLGRRPDNDWALP
                     NDTGVSREHCRIVHDEDGFALIDNSSFGSIVNDRRLGRGEHAQLREGDRITLGEYVFV
                     VSLEGEEPPFSGQTRMVESPQPAFPELDLENDPLGPFDPAAASPFSDPFQQKTLPEDV
                     TYPDDLPAGKEAFALPKRILLPDDADILGDLRPAVEAARPAAPQADGLSEFLKGAGLN
                     RVETGDSNAKLRIAGEAFRAAIEGICAILTARAQFKTELRVDKTQFGAARNNPLKSKS
                     STDDIVATMIAGRKGFMPAPDAMHEALEDIQAHEMALIAAIQVALSQLLEQIDPERLE
                     QRLEKRSIFDGLPGLKKARLWEVYETTYAEIAGELAEDFHGTFGKAFAEAYAKYFRGK
                     "
     gene            10599..11930
                     /gene="tssK"
                     /locus_tag="VGM72_05960"
     CDS             10599..11930
                     /gene="tssK"
                     /locus_tag="VGM72_05960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HEY4264844.1"
                     /translation="MGADNKVIWSEGMFLRPQHFQQHDRYIERLVRERAAGVTPYAYG
                     VAELKVSREFLIGGKFGLAQCRGILPDGTPCDLMADGNHPEPLEIGPNIRNEIIYLTL
                     PERQPGRVEVASSGHETPAARFTASSFRVFDAVAGNDTSADLEVGKLQLRYALESSER
                     AGFVCLGVARIMEVRADRAVILDERYIPPALACAAIPALAGITIELQGLLHQRAEELA
                     GRATQASGGTAEIANFLLLQMINRYEPLIHHYSGLSELHPVSLYATLLEMAGEFSTFV
                     TRTRRPIAFPGYRHDDLQNSFGPVMAELRQALSAVIDQSAVPIPLQERAHGVRVAVLN
                     DKSLLNDASFVLAVKADVQDEHIRRNFSGLTTIGPVEQIVKLVNSALRGIPIRPLPVS
                     PRQIPFHAGTVYFELDRKDPLWRELKTSAAFAFHIGGDFPGLAMEFWAIRR"
     gene            11935..13266
                     /gene="icmH"
                     /locus_tag="VGM72_05965"
     CDS             11935..13266
                     /gene="icmH"
                     /locus_tag="VGM72_05965"
                     /inference="COORDINATES: protein motif:HMM:NF038228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HEY4264845.1"
                     /translation="MAEPDDPFRIDPERTVMRPMPGRPAVARPTPRAAPAPAAVVAAA
                     AEDISFSELFLPGLSQILAAAGPLFAIAVRIRGSAAEPDMAALRDRILQEFKLFQDRL
                     RAANVPAKTAQLAHRALCALIDDLVLNTPWGAHSNWRAATLFSVFHGQVTGGDWFFDA
                     LRQLATEPRANLDVLELMYLCLSLGFEGRLRVQSTGPSELARLRENLYTTIREQRGKF
                     ERELSPHWQGVKAAHTSLPSRIPIWVFAAAAAAVLMVIFIGLSVLLGVASNPVFAELA
                     QLPPANPPSIAGGTSLPKFASHADRLRQFLAPEISAHLVAVGETDRTITISLQGSGIF
                     GAGSANVEDRYVDVLLRVGAAIQDEPGTVTVLGHTDSTPIRTIRFPSNYELSLARAEA
                     SRDVMRSRLTDPQRLQAEGMGDTAPLVSNDTPQGREQNRRIEIVLAKPVAA"
     gene            13270..16836
                     /gene="tssM"
                     /locus_tag="VGM72_05970"
     CDS             13270..16836
                     /gene="tssM"
                     /locus_tag="VGM72_05970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HEY4264846.1"
                     /translation="MAQEKDEGSVFNRMAHFLIQRWVMSGIGALALALLLWFLGPLLA
                     FMSGVFVRLVFVAIIAVVWLVANLVLDLRAARRNSAMIAKATDVSQDKTAISDKASKE
                     ETDTLRERLEEALAELKKSQSGGRGAQYLYQFPWYILIGPPGSGKTTALTNSGLKFPL
                     SKKFGKDPLRGVGGTRNCDWWLSDEAILLDTAGRYTTQDSNESADQKAWLSFLRLLKE
                     FRPRQPINGALVAIGLPDLLQMPASERGSHARAIKSRLAELKREFGLKLPVYVVFTKA
                     DRIAGFTEFFDDLDRESREKVWGMTFELERDAQPANSPAEEFHPQFEQLVERLNQRLL
                     ERVHEERDPDRRGRIYGFPLQFGTLKPMLHDFLEEIFEATRFEDRQLLRGIYFTSATQ
                     EGTPIDRLMGAMSRAFGLADKAAPMLGGAGRSYFLTRLLKDVIFEEASLAGADPRAER
                     RERLIRRAALAAVAVVAFFLIGAWTVSYLGNSALIADEESAKNAYAVAAAPLVQPTVG
                     DENIAAIMPVLDRLRGLPAGYDTAKSSAPLLRRFGLNTAPKLESQAVRGYRRALNGML
                     LPRLLVGIETQLKTNIDKPDYVNVFLPLYLELGGQGPLDKTLVQSAVSTLWGPRAYPN
                     LPPAESTKRLQAHVDALVEEPVTPIALDAELLMRARGVVANIAFSARAYSAIKEGAAA
                     KALPEWRIADHIGAATNQILTRTSGKPLVQGVPGFFTYDGFNKVLLPQIPAAIKQVTD
                     ESWILGAQAGQPANQDMNALREQIVTAYAADFISVWDQVLGDVTIIPFRNAQDAADVL
                     GRLSQPSSPLKLFLVAAAQETTMNRPPGAANPVAAAAAGAQAALNAAVGPAAGVLGSL
                     APSADTAPKVAANAIDGHFQPLHDFVAGAAAGQSQLDDTMKQLAQASAEIGGGASGAA
                     TAPGGDAMARLGQQASRLPAPLGGILAKATGTSTDIAAGANRKSIQDIYASTVLPFCR
                     QAIDGRYPFARASTVDVGLSDFQQLLRPGGLLDMFFQTNLKTHVDTSSTPWKNQKANG
                     GDLGLLRATLAQFELAQKIREDFFGANGAAIAVEFTLSPIETGSDVTQGVLDVEGQTL
                     TFMRGQTPSQKMQWPAPNSTDRARLTLTRASGQPALVETMGPWAFFRLLDRAKMDASL
                     PDQMTIGFDVNGATASFRLRASSVRNPFHARNLAQFQCAAQL"
     gene            16839..17456
                     /gene="tagF"
                     /locus_tag="VGM72_05975"
     CDS             16839..17456
                     /gene="tagF"
                     /locus_tag="VGM72_05975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagF"
                     /protein_id="HEY4264847.1"
                     /translation="MRAGCFGKIPQRGDFVSSNLPRQFIDAWDEFLREFLFASRAAIG
                     DLWLESYLHGPIWRFIFEPAHLVPSAAAGILMPSVDRVGRYFPFTVAVMTPSVPQGGT
                     DDPWFKRAEEFALETLDDAFDPQTLSQRLEALGGPEGFTGTQHQGGAQWSTLGSQTLG
                     PLSFRAAALPRSAQCTVLLDGDWQRWDWRVERISIDAAHAAVASS"
     gene            17453..18175
                     /locus_tag="VGM72_05980"
     CDS             17453..18175
                     /locus_tag="VGM72_05980"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEY4264848.1"
                     /translation="MTALFFEAAGTTDVGLKRGANEDSLLLRDSACLWVVADGMGGHH
                     AGDWASQKIRDGLQNLTLTGDFDRDIALIGNAVHAINTVIFEDSEKLGAMMGSTVAVL
                     HCRGPRFAVFWAGDSRVYLHRGGELYRITTDHSEVQEMIAAGLIGEDEAMNHPRRNIV
                     TRAVGVSPELTLDGIADRIEAGDVFLLCSDGLTGVVTDVEIGECLEANPFPESVEALL
                     KLTFDRGAPDNVTVAVVLCKLA"
     gene            complement(18285..18467)
                     /locus_tag="VGM72_05985"
     CDS             complement(18285..18467)
                     /locus_tag="VGM72_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264849.1"
                     /translation="MDEALWVAEGEIASFFGIPYPTRSVIAQLENGVWSPVKLTADLR
                     NKVDRLSAWKGLIPSV"
     gene            complement(18499..19140)
                     /locus_tag="VGM72_05990"
     CDS             complement(18499..19140)
                     /locus_tag="VGM72_05990"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4264850.1"
                     /translation="MASIGFALPLQNPIPISLSIGAAFVAALLLFYAAVMLRSSLVSK
                     KRVCERLIAAFALSGHEHVLDAGCGRGLALIGCAKKLTTGKAVGIDLWTAKDQSNNHP
                     EATLANAAAEGVANLVEVKTGDITRLPFPDASFDAVISMIVIHNIPSRQVRDEALREL
                     VRVLKPGGRMGIFDLMHASRYAEVLQDAGMRVQYLSRDFLWLLPCRSLLAQKS"
     gene            complement(19278..19781)
                     /locus_tag="VGM72_05995"
     CDS             complement(19278..19781)
                     /locus_tag="VGM72_05995"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY4264851.1"
                     /translation="MHDTLQSLFRYKSWANDELLTTLARLGGESSVTGLAIKALSHSY
                     IVDRIFAAHMRRRAHAYTSANASEMPTLEDLSADIGKSDREYIDYVSALDRDQLAERI
                     DFTFTDGARGRMSREEMLMHVVTHGIGHRGQVSAVMLLNSVPPAKDGFTTYLHEAEAS
                     ARRRTAA"
     gene            19904..>20448
                     /locus_tag="VGM72_06000"
     CDS             19904..>20448
                     /locus_tag="VGM72_06000"
                     /inference="COORDINATES: protein motif:HMM:NF036520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY4264852.1"
                     /translation="MARRPLTKPRKAASQERSRATVDALVEATARILVKEGFDKASTN
                     RIAEVAGVSVGSLYQYFPGKEALVVSVIERHQQETMQTVRGELAEVLAQPVEKAVRTL
                     IAVAVKAHRVDPKLHRVIAEQIPRVGKLEKVGTFSHENYMLFRTYLEGHRDELRVTDL
                     ELASFVCVTSIEALTHNAVLH"
BASE COUNT         3997 a         6154 c         6302 g         3995 t
ORIGIN      
        1 gtctccgaat tcatgacgag ataattgagc ccgcgccacg acccttcgag cttgagaaaa
       61 tccggatgat gcatgatcgc ggcgacctgc tgggaaagct gcttgtcgat cagctcgatc
      121 gccttgcgga aggtgagcgt catgttgcgg gaataggtta cggtgccttt cagggcctct
      181 tccgtgaggg ccttcagcag catctgcgcc tgttcgcggt cggtttgccg cgttgctccg
      241 atgacctgat cgagcaggtt ggcttcgccc gccgcttccg tggtttgagc ggtcttcgct
      301 gcagattgcg ttccgggatt gttggccatt attcgccctc tccagatctt ccgagtccga
      361 gctgttcgga caattttttc aaatcatctt cgttttgcag cacgcgttcg agcaagccct
      421 cgagttcggg gctgagatcg gccttggtgg caagatcgcg aagctggttg cgcgtttcca
      481 gcagcttctt cagggccggc acgctctcgg cgattttgcc cggctcgaaa tcgtccatcg
      541 accggaattt gagctgcacg gccatctcgg agccgtcgtc cttcatcgtg ttgttaacgc
      601 ggatgttcag ttccggtgag atacgcgcca tcacgtcatt gaaattatcg cggtcgatct
      661 ggacgaattt gcggtccttc agcggcggca acggcttgga gggattgccc gagaaatcgc
      721 ccatgacgcc gacgacaaac ggcagctccc gttcgacgac agcaccttcc tcttcgatct
      781 cgtatttgat gtgcacgcgc ggcttgcgaa cccgctcgag cttgtcattg atgctggcca
      841 tcaccgattc ctttcaatgt tgtccctgga ttcgatcaat tccaagacct gtcaagagcc
      901 ctcgggcggc ggcttgatgc cggcgctcgt caaggccgac cgccacgccg acctgtcggg
      961 caacaattcc atcagcaaat cggagaacga gaggcgcgcc cgccgcactg tctcttcgag
     1021 actgatcgcg atgggagagt gcggctcgtt ggcgtgaaaa tattgcgcga catgcaaaag
     1081 aacccgcaag gcatcctcgc ggctgtggat cccaccattg agattgtacc cgcccggctc
     1141 tgcggcggag ccattggcca aaggcaccac ttcgccgccg ccctcggagc gttgggagag
     1201 ctgggtcgcg cgcgccacga gatccttgct gaagcgctgg accgtttcca ggatggcgga
     1261 cagaacctcc cggattgccg atgtcggggg cgacgcgtcg cccgcacgct catcgagcgc
     1321 cgcgccgagt ctgttcagct cctcgatgct tgcctggatg tcgtcgatga gagcgatata
     1381 gaagacaccg ccgctggcat tggccaccgc ggtaaagcga tccagtgtca cgacgccgct
     1441 ttgttcccgg cgggcccgca cctccggatc ggcgatctcc gagagcgccc aagcctgctc
     1501 gtaatgatag gccgcaaagg gaccttcgcc gaccgctttg gtcagggaca ctttgcgaat
     1561 cggctgcaac agcgtgccgg tgccgtcggc gccgttcaac ccggtcagag gggccacctt
     1621 ggtcgcgacg ccttgttcat cctcgagcga atgaatcgta tcccaatatt gctcgaccaa
     1681 accccgtgcc aggcggcatc catcccgcag tccggggaag ccctgcaagc gaaccagcgc
     1741 ttcgatcagc caggcggtga cctcgagatc cttggagcgc tccgaaagaa ccgtcgtcgc
     1801 caaatcctgg attgtccgcc attcgggggt gacgcccaga tattcgcctt cggcgtccgc
     1861 gcgcttttcc gcgctgcggg cggcgctgcg ggcgtccttc aaacgaaaat agacggacgt
     1921 tggaacgacg tcctcgcgaa tattggcgcc agccggtgcg ccttcagaaa ttggcgccaa
     1981 tagctgttcg atatcgatgc acgggggcga gcccaccttt ggcccctttc caaggtgtaa
     2041 gcagaaattg tactgcccat taagctctta cgcatctttt ttatttcgcg cccactgcgg
     2101 agaattaggc acgaagccct tcacgcatcg ccccgtttgc ggcatcctcc cccaaatcgg
     2161 ggcaaagagt cttcgcggtt ttcgcaaatt tcggaaaagg gcagcggtcg cgacgccggc
     2221 cgaacacgta tcggctcctc aggatttggc gagtatttcg atttttttgt ccatcacggc
     2281 cgagccagga gagtcaagga tgagcagcaa ggcgatcgtt ctcaaacgca acaacagcga
     2341 cgctgcaaag ctcaaagccc ttcagcagct cgcgccgcaa tttctggtcg aatacagcag
     2401 cggtggacag ctcgcggccc tgacaaacaa ttttggaacg ctcgactact tgctggtcac
     2461 cggcgggcac ggcgattttg ttaataagaa gccgtcaaaa tttaacgatg ccgacatcgt
     2521 gtccgccaag aaatggattt ccaacgtgaa aggaacgttc aaggcgatca tcctggatac
     2581 ctgttttagc agcgcgctgg cgggatcctt tctgccattc ctgcccaatg gcggctgcct
     2641 tgtctgcgca cacggcaccg cggagggctg ggccgacggc ttcgtcaccg gaaacaacac
     2701 ccgcacgatc ggcgagcttc tggccgacgt catcgataat ttcgccggtt tgggcagctt
     2761 cgcgtcggtg agcctggcga ttaagaagcc gataaatcag gtgctctaca ccggcaacgg
     2821 gggcgccgac cgcaaggttg ggctggatgc gcgcagcaat atgggcatgg aggtcgacac
     2881 cgagcttgag ctcaaagagg tcgacatcta tctcgctcgc gaccgcatcg atgtggtcgc
     2941 gaccaagtcc gacgcgctca agaagctgct gaaggacaac atcacgacaa ccatcgtgtg
     3001 acagcgtttc gggcggaatt ctgcatgtcc tgattcggga caataataac ggacccctaa
     3061 gtcgccgcgg gcggcgtcat cacgtttgtg agatgtttat gggatcgtgc ccggcgatcg
     3121 tctaaggcta tcatggatcg gcagcgcata agggggctca tgtggccgca attgatctga
     3181 aatccttgat gggcaagctt aatgagcctt gccggcgcgc tttggaagcc gccgccggac
     3241 ttactttgtc gcgcacccat tacaatgtcg aagtcgaaca ttggctgttg aagctgctcg
     3301 atgtgcgcgg cggcgatctg gtcgcctgtc tcaaacatta cgaggtggac gaagcgcggc
     3361 ttgccgctga cctcaacaaa gtcctggacc ggctgaagac gggcaacagc cgcgcgccgg
     3421 ccttgtcgcc gcagatcgtg tccctgtgcc gcgaggcctg gctcacggcc tcgctggtgc
     3481 acggcgcggc gcgggtgcgt tccggacatc tgcttctggc actcctcagc gacgacatgc
     3541 tggcccagct tgcacgtgaa gcttcgggcc agttcaacaa aatcccgatt gactccctgc
     3601 gtgcggattt ttcgaaaatc attgccgaca cggtggaggc cgaagccggt gcaagcgcca
     3661 cctctggagc ccagggaagc agcgggcaat ctccggcagc ggcagccgga ggcgccctgg
     3721 accagtacac catcgatctg accgcgcgcg ctaaggcggg taaaatcgat ccggtgctcg
     3781 gccgtgacag cgaaatccgg caggtgatcg atattctgac tcgccggcgt cagaacaacc
     3841 ccattctcac aggcgaggcc ggtgtcggga aaacggccgt cgtcgaaggc tttgcgctca
     3901 agatcgcggc gggcgaagtc cccgacgcga tgaagaacgt cgcgttgcgg actctcgatc
     3961 ttgggctgct ccaagccggc gccggtgtga agggtgaatt cgagaaccgg ctcaaatccg
     4021 tcatcgatga ggtgaaggct tcgccgaccc cgatcattct gttcatcgat gaggcccata
     4081 cccttattgg ggcagggggc gcggcggggc agggcgatgc ggccaatctt ttgaagcccg
     4141 ctcttgcccg cggtgatttg cgtacaatcg ccgccacgac ctggggtgag tacaaaaaat
     4201 atttcgaacg cgatcccgcc ttggcccgcc gctttcaagt ggtgaaggtt gaggagccgg
     4261 acgaggctcc ggcgatagcg atgatgcgcg gtctggtcgg aatgctcgaa cagcatcacg
     4321 cggtgcgggt gctcgacgaa gcggtcatcg aggcggttcg gctttccaag cgctacatcc
     4381 cgggccgcca attgcccgac aaatcggtga gtgtgctgga tacggcctgt gcccgtgtgg
     4441 cgatgagcca gaacgccgtt cccgccgcta tcgaagactg ccgccggcgc atccagttga
     4501 tctccacaga gatggagatt ctgggacgcg aaaccgcctc cggtgccgac catgccgcga
     4561 cactcgaaca attgtccgcc ggcaagaaat ccgccgagga tgagctgcgc catttggata
     4621 tccggtgggc cgatgaaaat cgcctggtgg gaattcttcg cgagcttcga gacaaactcg
     4681 aagcggcgca catgggtaag gacggcggcg agaacctgag ttctgccggc agcatggccc
     4741 tgcgcgacga attcgaacgc acgaaacaaa aactcgtggc ggttcagggg gaagagccgc
     4801 tggtgcacgt cagcgtggat gcccaagccg tcgccgaagt catcgccaat tggaccggca
     4861 tccccgtggg ccgcatggtg tccaacgaga tcaagaccgt tctgtcgctc aaggaaacca
     4921 tgagtgagcg gatcatcggc cagccgcacg cgttggaggc gattgcgcag gcgatgcgca
     4981 cggcacgcgc gaaattgacc gacccgagaa agccgatcgg cgtgttcctg atggtgggca
     5041 ccagcggcgt cggcaagacc gagaccgcga tcaccttggc cgaactgctt tacggcggct
     5101 cgcagagcct gacggtcatc aacatgtcgg agttcaagga agagcacaaa gtctcgatgt
     5161 tgctcggctc cccgccgggc tatgtcggct ttggggaagg cggcgtcctg accgaggcgg
     5221 tgcgccggcg cccctatagc gtggtgctcc tcgacgagat ggaaaaagcc catcccggcg
     5281 tgcaggacgt gttctaccag gtcttcgaca agggccagtt gcgcgacggc gaggggcgcg
     5341 acatcgactt caagaatacc gttgtcatca tgacctcgaa cgccggcacg gagacgattg
     5401 ccaagctgtg cgccgatccc gaaaccatgc cggacgagaa cgggctcgcc gaagccttgc
     5461 ggcccgaact gctcaaggtc ttcaagcccg ccttcctggg ccgcgtgact ctggtgccct
     5521 attttccgct cagccccgaa acattgcgcc tcatcgtcaa attgcagctc gaccgcatac
     5581 agaagcggat cgcggaaaat taccgcgcga aattcgtcta cgcgcaggac gcgatcgaag
     5641 cgatcgccgc acgctgcacg gaggccgaaa gcggcgccag gaatatcgaa aacgtcatct
     5701 ctcgcggatt gttaccggag atttcggcgc gcttcctgga gaagatggcg aatggtgaat
     5761 ctttccgcca agtgcacctg tctatggaca ccgggggtgg cttcaaatat acgattgatt
     5821 gagggaattg attatccaga attcattctt gcacttgctg gcttcgatgt tttgggggac
     5881 tgcaagccgg caaatatgtg cgattgaacg cttcgccggc gatgccggta cttggattgt
     5941 gaaattggcc ggtaagatga aattggcccg actgatcatt ggggagattg catgccgatt
     6001 tatatgaaaa ttgatagcgt caatggcgag gtaaccgctg ccggatatga gaagtggatc
     6061 gacgtcggtt cgctgcaatt cggcatcggc cgggcgatat cgacgccggt gggcggcgga
     6121 tcgaaacggg aggcggggca cccctccatc agcgagatca cgctgacaaa gagtttcgac
     6181 aattcatcga tcgagctgtt cagctggtcg acggcaggca agtcgaaaac ggtgaagatc
     6241 gatatggtcc agaccggcga taaggacaag gtcagcgcct acctccagtt cgaactcagc
     6301 gacaccttga tttcgggtta ttcggtttcg tcgggtggag acaaaccgag cgagtcgatt
     6361 tcgctgaatt tcacgaaaat caccgagaaa tacacgcctt acggcaagga caacgcggtc
     6421 ggacagccta tcctgaaagg ctacgatctt gtcacggcca agacggtcta agcaggtgct
     6481 ttgcggccgc tcgcggtggg atgtcccgcg cgttcggccg ccttgtgttg gcagatgtgg
     6541 cggtggatgt cccatattgg atcggcagtc cttgaccgcg gggcggtttt ggacttttgc
     6601 cgccgagtga agcccgttgg ccggtcttga ggacatcgaa ctcgatcaga atgagcggct
     6661 gatttccgtc gagtcgtttc acctcgggaa gaaaaaaatc ctgatcaccg cgtttagcgg
     6721 cacggagcgg ctgtcggctc tgtcgcgatt ccagttggaa atcatgacga tcggccgggc
     6781 tctcgatcct ggcgagattc tcggcaagcc cataaccctc gccctgcgga tcaaagacca
     6841 agtccgcaag ttcagcggca tcgccggccg cttccagacc gtgcaaacct cgattcgcga
     6901 ttacaatttg cacatcgtcg aattggtgcc gccggcctgg ctcctctcgc tcaaccagcg
     6961 ctgcaaaatc tttccggaca aaaaagcgac cgacgttgtt tcccaagtcc tgaaagcagg
     7021 cggcgtcaac gtaaaagaga aaaccgccgg tacgtcgcgg gagtacatcg tccaatactg
     7081 cgaaagcgat ttcaatttca tttcacgatt gctcgaagac gagggcttgt tcttctattt
     7141 cgatcatggg gatccgaatt gcccgatcgt gctgggcaac ggttcggccg attattccag
     7201 agccggcctt ggcgcgctgg aattcaccgg ctgcatccag gagtgggagc cgcaatatca
     7261 ggtgggcgcc aatgccttcg agcatgcgga ttgggatttc aaagcggtaa agacgatcaa
     7321 cggcacctca aagggtcttg ccaaggtgca ggcgaagggc atcgcggacc gctcggtgta
     7381 cgaatatccc gggcgatacg gcaccgccga tgaggctacg gcgtgggccc gcgcccgcat
     7441 ggaagagcat gaagcaggcg cggtcaaaat ccgcgggacg acggcggata tcagggtgca
     7501 gccggcgtcc aagttcaaga ccaaggatta taagctccaa ttgccgggtt ctaccgacgc
     7561 aacggacagt tatgtctttg tttccgtgga gcaccgcgca cgcgacggtt ccggcgtgcc
     7621 gttcgaggcg cccacgagtt acgaaaacaa tttcgaatgc atcccggcgg acttgaattt
     7681 ccggccggcg cgcgtttccc atcgcccggc catcgccggg ccgcagaccg cgctggtgac
     7741 cgacgggccc gatgagtacg gccgcgcgaa agtcaaattt ccctgggagc agcaaggcca
     7801 gtcccgctgg acgcgcgtgg cgcagaattg ggcgttcaat cagatgggta cccaattcct
     7861 gccccgcatc aacagcgagg tggtggttga atttctggac ggcgatcccg atcagccgtt
     7921 gatcgtcggt atggtgtaca acgggaagaa caaattgccc tttgctgttc ccgctaacaa
     7981 aacccagagc ggaatccgcg gttcgaattg ggacgcggcc gggacggcca acaaaagcaa
     8041 tgagctgcgt ttcgaagaca aatccggatc cgaggaaatc ttcgtgcacg cgcaaaagga
     8101 cttcaagcgc gtcgtggtga acgatgattc gctcaccgtg gagcaaggca atcgaacgct
     8161 cgaaaccaag acgggaaacg tcaccgagac tttgagcaag ggcaatcaca gcctgaaagt
     8221 ggatgcgggc acgagcaagg tcgaggcgat gcagtccatc acgctgaaag tgggatcaaa
     8281 cagcgtggtc atcgatcagc agggcatcac gatcaaaggg atcatgatca agatcgaagg
     8341 ctccgcgatg gccaatatga aaagtcccat gaccgaagtg gacggcgatg gcatgctgac
     8401 cctcaagggc ggcattacca tgatcaactg agcgaggacg gtggaaatcg gagccctcac
     8461 caaggtcacg gcgcgtcagg cggacgaggt gttgcaactc gcgctggccg cggggcagag
     8521 cgtgctccag gttccaggga tggcgccgcc cgtctatctg cagcatctga tctcgaaacc
     8581 tgccttcaac gaggcggtgc aattccttgc ctttgccttg cccaaacgcg aagcggtgtg
     8641 gtgggcgtgc ctgtgcgcgc gcgccgattt gcacgagtcc gcgcccgccc cggtcgttgc
     8701 cgccctgacc gcggccgaag cctgggtcta tcgccccacc gaagagaatc gccatgccgc
     8761 catggagcgc gccaaggaag cgaaattcga aacgcccgcg gcctgggcgg cggtggctgc
     8821 gttttggagc ggccccagta tggcgcctcc cgacttgccg gccgtgcccc ctgcgcccca
     8881 tctgacgggg gtcgcggtct ttggcgcggt gacgcttgcg gccgtgcaga ccgaagcggc
     8941 attggcggac gaaaagcgtc agcgctatat taaaatcgcg gtcgatatcg ctaatggtgg
     9001 cagcggccgc gttgatgtga agggccgtta gggttttcct catgggtatg cctgccgcaa
     9061 ggctgacaga catgcatgtt tgcccgatgg tgacggggat cattccccat gtcggcggcc
     9121 cggtcatcat gggctcgccg acggtaataa ccggaaagat gccccaggcc agggtcagcg
     9181 atccgctgac ctgcgtcggt cctcccgata cgatcgtcaa gggcagcgcc accgtattgg
     9241 tgaattcctt gcccgctgcc cgcatcggcg atacgacggc ccatggcgga actattatcc
     9301 tgggcttccc gaccgtgctg atcggcggtt gagcatttcc tcgcgatgaa tctcattctc
     9361 accatcgtgc aaggcccgcc gggcggcgcg gcagcaacgc gcagcttcga cgatgcggcc
     9421 ctcggcaagc gtggtatcac gctcgggcgc cggccggata atgattgggc gctgcccaac
     9481 gacaccgggg tctcgcgcga acattgccgg atcgtccatg acgaggatgg gttcgccctc
     9541 atcgacaaca gcagctttgg ctcgatcgtg aacgaccgcc gcctgggacg cggagagcac
     9601 gcgcagcttc gggagggcga ccgcatcacg ctgggcgaat atgttttcgt cgtgtcgctc
     9661 gaaggggagg agcccccttt ttccgggcag acgcgaatgg tcgaatcgcc gcaaccggcc
     9721 tttcccgaac tggacctgga gaacgatccg ctggggccct tcgatcctgc cgcagcatcc
     9781 ccgttcagcg atccttttca gcagaagacg cttccggagg atgtcaccta tcctgacgat
     9841 ctccccgccg gcaaggaggc cttcgcgctt ccgaagcgca tcctgcttcc ggatgacgcc
     9901 gatattcttg gcgatcttcg gcccgccgtc gaagcggccc ggcccgccgc gccgcaagcc
     9961 gatgggttgt ctgagttctt gaagggggcg ggcctcaacc gtgtcgaaac cggcgattcc
    10021 aacgccaagc tgcgaatcgc cggtgaggct ttccgcgccg ccatagaagg catctgtgcc
    10081 attctgaccg cccgcgcgca gttcaagacc gagttgcggg tcgataaaac acagttcggt
    10141 gccgcccgga acaatccgct caaatcgaaa tcctccacgg acgatatcgt cgcgaccatg
    10201 attgccggcc gcaaaggttt catgcccgca cccgatgcga tgcacgaagc gctggaagac
    10261 atccaggccc acgagatggc gctgatcgcc gcaatccagg ttgccttgag tcaattgctg
    10321 gagcagatcg acccggaacg tctcgagcag cggctcgaga agcgttcgat ttttgacggt
    10381 ttgccgggat tgaaaaaggc tcggttgtgg gaagtctacg agaccaccta cgcggaaatc
    10441 gccggcgagc tggccgagga ttttcacggc acgttcggca aggccttcgc ggaggcctat
    10501 gccaaatact ttcgtggaaa gtagccgcgt ccggtagtat gaaagcgggg ggcgattttt
    10561 ccgcttccgg gacatttttg cggcagatcg gcagaggaat gggggctgac aacaaagtca
    10621 tctggtccga aggaatgttc ctgcggcccc agcattttca gcaacatgac cggtatatcg
    10681 agcgcctggt tcgcgagcgg gccgccggcg ttactcccta tgcttatggc gttgcggagc
    10741 tgaaagtaag ccgcgagttt ttgatcggcg ggaagttcgg gctcgcgcaa tgccggggca
    10801 tattaccgga cgggacgcca tgcgatctca tggccgacgg caaccatccc gaaccgctcg
    10861 aaatcggccc gaacatccgc aacgaaatta tctatctcac tctgccggag cgccagccgg
    10921 gccgggtaga agtggcgagc agcggccatg agactcctgc ggcccggttt accgcgtcat
    10981 cctttcgcgt tttcgatgcg gtcgcgggaa acgacaccag cgccgacctg gaggtcggaa
    11041 agctccagct ccgctacgca ctcgaatcga gcgagcgggc gggctttgtc tgtctcggcg
    11101 tcgcccggat catggaagtg cgcgccgaca gggccgtcat cctggatgag cgctacattc
    11161 ctccggcgct ggcctgcgcg gcgattccgg cgctcgcggg aatcaccatc gagcttcagg
    11221 ggttgctgca tcagcgcgcc gaagaattgg cggggcgtgc aacacaggcg tcgggcggca
    11281 cggcggagat cgccaatttc ctcctgctgc agatgatcaa tcgctatgag ccgctgatcc
    11341 atcattattc cgggctatcc gagcttcacc cggtgagtct ttacgcaacg ctgctcgaaa
    11401 tggcgggcga gttttccacc ttcgtgacgc gcacccgccg ccccatcgcc tttcccggtt
    11461 accgccatga cgatctgcag aacagttttg gccccgtcat ggccgaactc cgtcaggcac
    11521 tgagcgcggt catcgatcaa agcgcggttc cgattccgct gcaggaacgt gcccatgggg
    11581 tgcgggtcgc cgttctgaac gacaaatcgc tgctcaacga cgccagcttc gtgctggcgg
    11641 tgaaggccga tgtgcaggat gaacacatcc gccgcaattt ctccggtctc accaccatcg
    11701 ggcctgtcga gcagatcgtg aagctcgtga acagcgcgct tcggggaata ccgatccggc
    11761 cgcttccggt aagtccgcgc caaattccct tccatgccgg aacggtttat tttgagctgg
    11821 accgaaagga tccgctgtgg cgagagttga aaacttcggc cgcattcgcg ttccatatcg
    11881 gcggtgattt cccaggtctt gccatggaat tctgggcgat acggagatga gcctatggcc
    11941 gaacccgacg atcccttccg tatcgatcct gaaagaaccg tcatgcgtcc catgccggga
    12001 cgcccggccg tggcgcggcc cacgccgcgg gctgcgccgg cgccggcggc agtcgttgcc
    12061 gccgcggccg aagacatctc gttttcggaa ttgttcctcc cgggcttgag ccagatcctt
    12121 gcggctgccg gccccctgtt tgccatcgcc gttcgcatcc gcggctcggc ggccgagccc
    12181 gatatggcgg cgctgcgcga tcgcatcctt caggaattca aacttttcca ggacaggctt
    12241 cgcgcggcaa acgtcccggc gaagacggct cagttggctc atcgggcgct gtgcgcgctg
    12301 atcgacgatc tggtgctcaa tacgccatgg ggcgcgcaca gcaattggcg ggcggccacg
    12361 ctgttcagcg tctttcacgg tcaggtcacg ggcggggatt ggttcttcga tgccttgcgg
    12421 cagctcgcta ccgagccgcg agcgaatctg gatgttctcg aactgatgta tctctgcctc
    12481 tcgctcggct tcgaaggacg gctccgggtg caatcgacgg gcccgtctga actcgcgcgc
    12541 ctgcgcgaaa acctctacac gaccatccgc gaacagcgtg gcaaattcga acgggaattg
    12601 tcgccgcatt ggcagggtgt caaggcggct cacacctcgc ttccgagccg gattccgatt
    12661 tgggttttcg cagccgccgc ggcggccgtt ctgatggtca tcttcatcgg gctcagcgtt
    12721 cttcttggtg tcgcatccaa tccggtcttc gccgagcttg cgcaattgcc gcccgccaat
    12781 ccgccatcca ttgccggcgg caccagcctg cccaaattcg ccagccatgc cgaccggctg
    12841 cgccagttcc tcgctccgga aatcagcgct catctggtgg cggtcggcga aaccgaccgc
    12901 acgatcacaa tctcgcttca gggaagcgga atattcggcg ccggcagcgc caatgtggaa
    12961 gatcgctatg tcgatgttct cctgcgcgtc ggcgcggcga tacaagacga gccgggcacc
    13021 gtgaccgtgc tcgggcacac cgacagcaca ccgattcgca ccatccgttt tccgtccaat
    13081 tacgagctgt cgctggcgcg ggccgaagct tcccgcgacg tgatgcggag cagattgacg
    13141 gacccgcagc gcctccaggc cgagggcatg ggcgacaccg cgccgctcgt ttcgaacgac
    13201 acgccccagg gccgcgagca aaaccggcgc atcgagatcg ttctcgccaa gccggtcgcg
    13261 gcgtgaacga tggcgcaaga aaaggatgag ggctcggtgt tcaaccgcat ggcgcatttc
    13321 ctgattcagc gctgggtcat gtccggcatc ggcgctttgg cgctggcact gctgctgtgg
    13381 tttctgggtc cgctcttggc attcatgtcg ggtgtcttcg tgcgcctcgt ctttgtggcc
    13441 atcattgcgg tggtctggct ggttgcgaat ctcgtcctcg acctgcgggc tgcgcgccgc
    13501 aacagcgcga tgatcgccaa ggcgacggat gtttcgcaag acaagaccgc gatctcggac
    13561 aaagcctcca aggaggaaac cgacacgctg cgcgagcgtc tcgaggaagc gcttgccgag
    13621 ctgaagaagt cccaaagcgg aggccgcggc gcccaatatc tgtatcagtt tccctggtac
    13681 atcctgatcg gcccgccggg atcgggaaaa accaccgcgc tcaccaattc gggtttgaag
    13741 tttccgctgt cgaagaaatt cggcaaggac ccgctgcgcg gcgtcggcgg cacacgcaat
    13801 tgcgattggt ggctttccga cgaagccatc ctgctcgata cggcaggccg ctacacgacc
    13861 caggacagca atgaatcggc cgaccagaaa gcctggctgt cgttcctgcg tctgctgaag
    13921 gaatttcgcc cgcgtcagcc gatcaatggt gctctcgtcg ccataggcct gccggatttg
    13981 ctgcaaatgc cggcgtcgga acgcggcagc cacgcgcgcg cgatcaagag ccggctcgcc
    14041 gagctgaaac gcgaattcgg gctgaaattg ccggtgtatg tggtgttcac caaagccgac
    14101 cgcatcgccg gcttcaccga attcttcgac gatctggacc gcgaatcgcg cgagaaggtc
    14161 tggggcatga cgttcgagct cgaacgcgac gcccaacccg cgaattcgcc ggccgaagaa
    14221 ttccatcccc aattcgaaca attggtcgag cgcctgaacc agcgtcttct ggagcgagtt
    14281 cacgaagagc gcgaccccga ccgccgtggc cgcatttatg gatttccgct gcagttcggc
    14341 acgctgaagc caatgctgca cgatttcctg gaggagatat tcgaagccac gcgctttgag
    14401 gaccgtcagc ttttgcgcgg tatatatttt accagcgcga cccaggaagg cacaccgatc
    14461 gatcggctga tgggggcgat gtcgcgggcc ttcggcttgg cggacaaagc ggccccgatg
    14521 cttggcggcg ccgggagaag ctatttcctc acgcgcctgc tgaaggatgt catctttgaa
    14581 gaagccagcc ttgccggcgc ggacccgcgc gcggagcgcc gcgagcgcct gatacgccgc
    14641 gcggcgctgg ccgccgtcgc cgtggtggcg tttttcctga tcggcgcgtg gacggtgagc
    14701 tatctcggca attcggcgct gatcgccgac gaagagagcg ccaaaaacgc gtatgccgtc
    14761 gcggcggctc ctctggtgca accgaccgtc ggcgatgaga acatcgccgc gattatgcct
    14821 gtgctcgacc gcttacgcgg cttgccggcc ggctatgaca cggccaaatc cagcgcaccc
    14881 ttgctgcgcc ggttcggcct caacaccgcc ccgaaactgg agagtcaggc ggtgcgcggc
    14941 tatcgccggg cgctgaacgg catgttgctg ccgcgcctgc tcgtcggtat cgagacgcag
    15001 ttgaagacca acatcgacaa acccgactac gtgaatgtgt ttctgccact ctacctcgaa
    15061 ctcggcgggc aagggccgct cgacaagaca ctcgtccaat ccgcggtctc cacgctatgg
    15121 gggccgcgcg cctatccgaa tcttccgccg gcggaaagca ccaagcggct tcaggcgcat
    15181 gtcgatgcgc tggtcgaaga gccggtcacg ccgattgcgc tcgacgccga attgctgatg
    15241 cgcgcgcgcg gcgttgtcgc gaacattgcg ttctccgcgc gcgcctatag cgcgatcaag
    15301 gaaggcgccg cggcgaaagc gctgcccgaa tggcgcatcg ccgatcatat cggcgctgcc
    15361 accaatcaga ttctgacacg cacgtccggc aaaccactgg tgcagggcgt gccgggattt
    15421 ttcacctatg acgggttcaa caaggttttg cttccgcaaa ttcccgcggc gatcaaacag
    15481 gtcaccgacg agagttggat cctgggcgcg caagcaggcc agcccgcgaa ccaggatatg
    15541 aacgccctgc gggaacagat tgtcaccgcc tatgcggccg atttcatttc cgtctgggat
    15601 caggttcttg gcgacgtcac gatcattccg ttccgcaatg cccaggatgc cgccgacgtc
    15661 ctgggccggt tgtcgcaacc gagttcgccg ctgaagcttt tcctggttgc cgcagcgcag
    15721 gaaaccacca tgaatcgccc tccgggcgcc gccaatcccg tggcggcggc agcagcgggc
    15781 gcacaggccg cgctcaatgc ggcggtggga cccgcggccg gggtcctggg cagtctcgcg
    15841 ccgtcggccg ataccgcgcc gaaagtggcc gccaatgcca tagatggcca tttccagccg
    15901 ctgcacgatt tcgtcgcggg cgccgccgcc ggacaatcgc agctagacga tacgatgaag
    15961 cagttggcgc aagcttcggc cgaaatcggc ggcggcgcgt ccggcgctgc cacagcaccg
    16021 ggaggcgacg ccatggcgcg gctcggtcag caggcatccc gattgcccgc gcctttaggg
    16081 ggcattcttg ccaaagcgac ggggacctcg accgatatcg cggcgggcgc caatcgcaaa
    16141 tccattcagg acatctacgc ctcgaccgtg ttgccgttct gccggcaggc gatcgacggg
    16201 cgctatcctt tcgcgcgcgc cagcacggtt gacgtgggcc tgagcgattt ccagcagctt
    16261 ctgcggccgg gcggtctttt ggacatgttc ttccagacca atctcaagac ccatgtcgat
    16321 acgtccagca cgccgtggaa gaaccagaaa gccaatggcg gcgatctcgg attgttgcgc
    16381 gcaacgctgg cgcagttcga actggcgcag aaaatccggg aggatttttt tggcgcaaac
    16441 ggcgccgcga ttgcggtgga atttacgctc tccccgatcg agaccggcag cgacgtgacg
    16501 caaggggtcc tggatgtcga agggcagact ctgaccttca tgcgcggtca gacgccctcg
    16561 cagaaaatgc aatggccggc gccgaacagc acggaccgcg cgcgccttac cttgacgcgc
    16621 gccagcggtc agccggcgct cgtggagacg atggggccat gggcgttctt ccggctgctc
    16681 gaccgcgcaa aaatggatgc gagtttaccc gatcagatga cgataggatt cgacgtcaac
    16741 ggcgcgaccg cgagcttccg gctgcgcgca tcgagcgtgc gtaatccctt tcacgcgcgc
    16801 aacctcgcgc aatttcagtg tgctgcccag ctgtgacgat gcgcgcggga tgtttcggaa
    16861 aaattccgca gcgcggcgat ttcgtgagca gcaatctgcc gcgccaattc atcgacgcgt
    16921 gggacgagtt tctgcgggag ttcctgttcg cgagccgggc ggcgatcggg gatttgtggc
    16981 tggagtccta tcttcatggc ccgatctggc gtttcatctt cgaaccggcg cacctggttc
    17041 cgtccgccgc tgccggcatc ctgatgccga gcgtggatcg cgtgggccgc tattttccct
    17101 ttaccgtcgc tgtgatgacc ccatccgttc cgcaaggggg gacggatgat ccatggttta
    17161 agcgcgcgga ggaattcgcg cttgagacgc tggatgatgc attcgatccg caaactctgt
    17221 cgcaacgttt ggaggctctg ggagggccgg aagggttcac cggcacgcag catcaaggcg
    17281 gcgcccagtg gtcgacgctg ggttcccaaa cgcttggacc gctttccttt cgtgcggcgg
    17341 ctttgccccg gtctgcccaa tgcaccgttc tcttggatgg cgactggcag cgctgggatt
    17401 ggcgcgtcga acgcatttcc atcgacgccg cacatgcggc ggtggcatcg tcatgaccgc
    17461 gcttttcttt gaagcggcgg gaacaacgga tgtaggcctc aagcgggggg cgaatgagga
    17521 ctctctgctg ctgcgcgatt cggcgtgcct gtgggttgtt gccgatggta tgggcggaca
    17581 ccacgccggc gactgggcat cgcagaaaat tcgcgacggt cttcaaaatc tgactttaac
    17641 gggagatttt gaccgggaca ttgctcttat cggcaatgcc gtccatgcga tcaacaccgt
    17701 gattttcgaa gattccgaaa agctcggggc catgatgggc agcacggtcg ccgttctgca
    17761 ttgccgcggt ccgcgtttcg ccgttttttg ggccggagac agccgcgtct atctccaccg
    17821 cggcggtgag ctgtatcgca tcaccacgga ccactccgag gttcaggaga tgatcgcggc
    17881 aggcctcatc ggtgaagatg aggcgatgaa ccatccacgg cggaacatcg tgacgcgggc
    17941 tgtcggtgta tcgcccgaac tcacgctgga tggcatcgcg gatcggatcg aagccggaga
    18001 cgtctttctc ctgtgcagcg atggtctgac cggcgtcgtc acggacgtgg agatcggcga
    18061 atgcctggaa gccaatccgt tccccgaaag cgtggaagcg cttctgaaac tcaccttcga
    18121 tcgcggcgca ccggacaatg tcacggtcgc agtggttctt tgcaagctgg cgtgaaattt
    18181 tcgggcggct gccttctgga agcccctcta taggcctcac ggagtttaca cgcggaaccg
    18241 tcacgacgtc atgatgggag cggcggcatg acggtctgcc gccatcatac gctgggaata
    18301 aggcccttcc acgccgacag acgatccact ttgttgcgca gatccgcagt tagcttgact
    18361 ggcgaccata ccccgttctc caattgggcg atgacggaac gcgtcggata gggaatgcca
    18421 aagaagctgg caatctctcc ttcggcgacc caaagagcct catccacttg ctgcgctggc
    18481 ttggatggaa gcgacgcgct acgatttctg cgcgagcagc gagcggcaag gtagaagcca
    18541 caggaaatca cgagacagat actgaacgcg catgcctgca tcttgtaaca cttcggcata
    18601 tcgagacgcg tgcatgagat cgaaaatgcc catgcgaccg ccgggcttca gcacgcgaac
    18661 gagttcgcga agcgcctcat ctcgcacctg tcgcgaagga atattgtgaa tcacgatcat
    18721 tgagatgacg gcgtcgaacg acgcgtcggg aaacggtagt ctggtgatgt cgccggtttt
    18781 cacctcgacc aggttggcga cgccttccgc tgccgcgttg gccagcgtcg cttccggatg
    18841 gttatttgac tggtccttgg cggtccatag atcgataccg accgccttgc cggtggtgag
    18901 tttttttgcg caaccgatga gcgccaatcc acggccgcat ccggcgtcga gcacgtgctc
    18961 atgtccggaa agcgcaaacg ccgcgatcaa gcgctcgcat acccgcttct tgctcacaag
    19021 gctcgatcgc aacatgaccg ccgcgtagaa cagaagaaga gccgcgacaa aagcagcacc
    19081 gatgctcaat gatatgggaa tagggttctg caggggcaat gcgaagccga tcgatgccat
    19141 gaccgccagg acgcatccga aaacaatatg agcgcggacc actccaggag cgtcgacgcc
    19201 atatgctgcg cgttgtttca tggtgttcga agcctcaact tgtcaacatg cggctcgtgg
    19261 ttccgtaacg cgctggctca cgccgcagtt cgccttcttg ccgatgcttc cgcttcatgc
    19321 aggtacgtcg tgaaaccatc cttcgctggt ggaacggaat tgagcagcat gactgcgctg
    19381 acctgtccgc gatgacctat gccgtgggtg accacatgca tgagcatctc ctcgcgggac
    19441 atacggcccc gcgcgccgtc cgtgaacgtg aagtcgatcc gttccgccaa ttggtcgcga
    19501 tcgagcgccg acacataatc gatatactcc cgatcgcttt ttccgatgtc cgcggacaaa
    19561 tcttccagcg tcggcatctc gcttgcattg gccgatgtgt acgcgtgggc ccttcgccgc
    19621 atgtgcgcgg caaagatccg gtcgacgata tagctgtggc tcaaggcctt gatggccaat
    19681 ccggttaccg aggattcgcc gcccagtctg gccaacgtgg tcaacaactc gtcgttcgcc
    19741 caggatttat atcggaacaa gctctgcaat gtgtcgtgca tgtcgaggtc tccttgggat
    19801 ttgcgcgttg cgcgtgttgc gcaaggcgcg gccttgcatt tccacgccgg acagaatatg
    19861 agcgattatt cgcttttgat actcgcattg gcggcgtgga aaaatggcgc gcagaccgct
    19921 cacaaaaccc aggaaagccg cttctcagga gcgatcgcgc gcgacggtcg atgcgctggt
    19981 tgaagcgacc gctcgcattc tcgtcaagga aggtttcgac aaggcaagca ccaatcgcat
    20041 cgcggaagtg gcgggggtga gcgtcggctc cctctatcaa tatttccccg gcaaggaggc
    20101 gctcgttgtc tctgtcatcg agcgtcacca gcaggagacc atgcaaacgg tccggggcga
    20161 gttggcggag gttttggccc agccagtgga aaaagcggtg cgcacgctca ttgctgtcgc
    20221 ggtgaaagcg caccgcgtcg atcccaagct gcatcgtgtg atcgcggagc agattcctcg
    20281 cgtaggcaag ctcgagaagg tggggacctt cagtcacgag aactacatgc tgttcagaac
    20341 gtatctcgag ggccaccggg acgaacttcg tgtcactgac ctggagctcg catcgtttgt
    20401 atgcgtgacc tcgatcgagg ctttgacgca taacgcggtg ctgcacta
//
LOCUS       DASYEG010000136         5029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4824375, whole genome shotgun sequence.
ACCESSION   DASYEG010000136 DASYEG010000000
VERSION     DASYEG010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4824375"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VGM72_06005"
     CDS             complement(<1..361)
                     /locus_tag="VGM72_06005"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HEY4264853.1"
                     /translation="MSIPQSAGGPISSRADLVAHIAAGSKPREQWRIGTEHEKFVYGL
                     NDRKPLPYDGPSSIRALLEGMERFGWKPILEGENIIGLSQNGAALSLEPGGQFELSGA
                     PLKTVHETCVEVNTHLKQ"
     gene            complement(439..1254)
                     /locus_tag="VGM72_06010"
     CDS             complement(439..1254)
                     /locus_tag="VGM72_06010"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEY4264854.1"
                     /translation="MGQDKAQDSASDDLPYADLTYSGGKLRLFVESPLHAGARIAPDE
                     GQAHYLLHVMRAREGQRVLLFNGSDGEWQARIAQVSRRSCVLICETQSQAQAAVPDLW
                     FAFAPIKKTPADYVAQKATELGVRVLQPVFTHRTITRRVNLDRLRANAVEAAEQSQRL
                     SVPEIREPVGLDVLLRSWPAERRLLFCDEGGDAAPVAEALQQAAAGNMSWGILIGPEG
                     GFDAQERAAIRNHPFVLAVSLGPRIMRADTAALAALAVWQAIRGDWGQSRPGL"
     gene            1295..2251
                     /gene="ubiA"
                     /locus_tag="VGM72_06015"
     CDS             1295..2251
                     /gene="ubiA"
                     /locus_tag="VGM72_06015"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR01474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEY4264855.1"
                     /translation="MSADPQAPENPLRASRPGDALATSWVDAAPNALQPYFRLMRLDR
                     PIGAWLLYWPCVFGLALGAAAAGRAYPDPLHVVLTGIGAVVMRGAGCTYNDIVDRDYD
                     ARVARTRGRPIPSGAVSVRQAWAFALALSFVGLLILLTFNRFAILLGVGSLFLVACYP
                     FMKRITWWPQAWLGLTFNWGVLFGFAAATGTLSYAAFLFYAGCFCWTLGYDTIYAHQD
                     KEDDILIGVKSSALRRGTRTLTWIAGFYGAALALMIAGGGAAGLGIVFVIAMLVPAAH
                     LLWQLGRLDIDSPLVCLRLFKANRDTGAAIAVALVIGMLVRA"
     gene            2248..2940
                     /locus_tag="VGM72_06020"
     CDS             2248..2940
                     /locus_tag="VGM72_06020"
                     /inference="COORDINATES: protein motif:HMM:NF021788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY4264856.1"
                     /translation="MTVFSDTPTSDSESRIAFVRANTILTAPPIVPEIKLHLASEVMN
                     LWTETEARSAALASGVAHLPPPYWAFAWPGGQAVARYVLDHPETVAGKTVLDFGAGSG
                     IVAIAAAKAGGSPVIAAEIDPLALGAMELNAAANGVFIEPVENDIIGTNGRWTTILAG
                     DMCYERPLAERLLDWLRTLTAGGARVLIGDPGRNYFPGHGIERLATYPVPTTRDLENR
                     ELRETSVYRLLP"
     gene            3015..4898
                     /gene="nhaA"
                     /locus_tag="VGM72_06025"
     CDS             3015..4898
                     /gene="nhaA"
                     /locus_tag="VGM72_06025"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEY4264857.1"
                     /translation="MAEEIAATSLLQHAIDPTRDHIRGNKRPDSVSIVLYGDYLCPYC
                     RRLRFVIARLRQALGDRLIYVFRHFPNERAHPGAEAIARASEAVERQGRFWELHDWLY
                     AQEPGDIPGGVTDFIRSLGIDMERYERDLADSAIQKRVDEDLADGKRNGVTATPTVFI
                     DGYRYDGAWDFYSMLETLERPVAARVQRSARAFASLPASGGLVLLLAAAAAILCANTA
                     LAPYYRLFIDSTVGIGTPGSMLTLTVGAWFSEGLLSIFFLLVGLEIRREMTAGALSDT
                     RAAILPVLAAVGGVLAPTAIYLALNPASTARGWSVPTATDIAFTLGILALLGNRITPG
                     LRVFVAALAVVDDILSVLTLAIFYPRAFEISWLFTSALTIILLYALNRWRVYAASPYV
                     LVAVLLGISLHGAGVHAALAGVILAGFLPTRPAPETGPLLAQAATALAALEHAENEAK
                     ESGENRRIEQEPIWDWASRNLSAASDRLLSPADRIERAVSPWSAYVILPLFAFSATGV
                     GLNVDLAAPGALPILLGVILGLVIGKPLGISLASLLAIKTRIARAPEDVTLRNFIGAA
                     CLCGVGDTVALLMADQAFPQGPDAAVAKIGVLIGSVLAAGLGAAILAPSPPIHAESPL
                     PQG"
BASE COUNT          773 a         1648 c         1602 g         1006 t
ORIGIN      
        1 cctgcttcag atgcgtgttc acctcgacgc aggtctcatg caccgtcttg agcggggccc
       61 ccgaaagctc gaattgcccg cccggctcca ggctcaacgc ggccccgttt tgcgacaggc
      121 cgatgatgtt ctcgccttcc aggatgggct tccagccaaa gcgctccatg ccttccaaaa
      181 gcgcgcggat gctcgatggt ccgtcgtagg gcagaggctt gcgatcgttc aggccgtaga
      241 cgaatttctc gtgctccgtg ccgatccgcc attgctcgcg cggcttcgaa cccgcggcga
      301 tatgggccac gagatcggca cgcgaagaga tcggcccgcc ggcggattgc gggatggaca
      361 tgcgggtttc ctctgtccgc gatctaaact tcgcggtttg cggtgggtcg acgcgaacct
      421 agtgtcgcgc ccggcgagtt acaagccggg acgggattgc ccccaatcgc cgcggattgc
      481 ctgccaaacc gccagcgccg ccagcgcggc cgtgtctgcg cgcatgatcc tgggaccgag
      541 cgatacggca aggacgaagg ggtggttacg aatggcggcg cgctcttggg cgtcaaatcc
      601 cccttccggc ccgatcagga taccccagga catgtttccg gcagcagcct gttgcaatgc
      661 ctcggccacc ggcgccgcat cgccgccctc atcgcagaag agaagccgcc tttcggccgg
      721 ccaggaccgc agcaggacat cgaggcccac gggctcgcga atctccggca cgctgagtcg
      781 ctgggactgt tccgcagcct cgacggcgtt ggcgcgcagg cggtccagat tgacccggcg
      841 cgtgatcgtg cgatgcgtga ataccggctg gagaacccgc accccgagtt cggtggcctt
      901 ctgcgccaca tagtcggcag gagttttctt gatcggcgcg aacgcgaacc aaagatcggg
      961 caccgcagct tgcgcctggc tctgcgtttc gcagatcagc acgcaggaac gccgcgaaac
     1021 ttgcgcgatg cgggcctgcc attcgccatc gctgccgttg aacagtagca cgcgctgacc
     1081 ctcgcgggcg cgcatcacat gcagaagata atgcgcctgc ccctcgtccg gcgcgattct
     1141 agcgcccgca tgaagcgggc tctcgacgaa aagccgcaat ttgccgcccg aataggtcag
     1201 atcggcatag ggcagatcgt cgctcgcgga gtcctgggct ttgtcctgcc ccataggcgc
     1261 gctaccctgt tgtcttccca acgatgcagc acgcatgagc gccgatccgc aagcccccga
     1321 gaatccgttg cgcgcctcac ggcccggtga tgcgctggct acgagttggg tggacgcggc
     1381 gccgaacgcg ctccagcctt atttccggct gatgcggctc gaccgcccga taggggcctg
     1441 gctgctctat tggccctgcg tgttcggact tgcgctgggc gcggccgctg ccggcagggc
     1501 ctatcccgat ccgcttcatg tcgtgttgac gggaattggg gccgtcgtga tgcgcggcgc
     1561 gggctgcacc tataacgaca tcgtcgaccg cgattacgat gcgcgcgtcg cacgcacgcg
     1621 cgggcggccc atcccatcgg gcgctgtgag cgtgcgccag gcctgggcgt tcgcgctggc
     1681 gctttctttc gtcgggttgc tgatcctgct gacgttcaat cgtttcgcga tcctgctggg
     1741 cgtgggctcg ctgttccttg tcgcgtgtta tcccttcatg aagcgcatca cctggtggcc
     1801 ccaggcctgg ctcgggctca ccttcaattg gggcgtgctt ttcgggtttg ccgcggcaac
     1861 cggaacgctt agctacgcgg cgttcctgtt ctatgcgggc tgcttctgct ggacgctcgg
     1921 ctacgacacc atctatgcgc atcaggataa ggaagacgac atcctgatcg gcgtgaagtc
     1981 atcggccttg cggcggggca cgcgcacgct cacttggata gctggatttt atggggcagc
     2041 cctcgccctg atgatcgccg gggggggggc agcgggatta ggtatcgtct tcgtcattgc
     2101 catgctggtt cctgctgcgc acctgctctg gcagttgggc cgcctcgata tcgacagccc
     2161 gctcgtttgc ctcagactgt tcaaggccaa ccgcgacact ggtgctgcca tagcggtggc
     2221 tcttgtcatc ggcatgctgg tccgcgcgtg acggtcttct ccgacacgcc cacttccgac
     2281 agtgaaagtc gcatcgcctt cgtgcgcgcc aataccatcc tgacggcgcc gcccatcgtg
     2341 ccggagataa aacttcatct cgcgagcgag gtgatgaatc tctggaccga gaccgaagcg
     2401 cgttccgccg cgcttgcatc gggcgttgcc catctgccgc cgccctattg ggcgtttgcc
     2461 tggccgggcg gccaggccgt cgcgcgttat gtcctggatc atcccgaaac cgtggccgga
     2521 aaaaccgtgc tcgattttgg agccggttcc gggatcgtcg cgattgccgc cgcaaaagcg
     2581 ggcggcagtc cggtcattgc cgcggagatc gatccgctcg cgcttggcgc gatggaactc
     2641 aatgccgcgg ccaatggtgt tttcatcgag ccggtcgaaa acgacatcat cggcaccaat
     2701 ggccgttgga cgacgatcct cgccggcgac atgtgctatg agcgccctct tgccgagcgc
     2761 ctgctcgact ggctgcgcac cctcactgcc ggcggcgcgc gcgtgctgat cggcgatccg
     2821 ggacgcaatt attttccggg gcacggtatc gaacggctcg ccacctatcc ggtgccgacc
     2881 acgcgcgatc tcgagaatcg cgagctgcgg gagacgtcgg tgtatcggtt gctgccctag
     2941 acatttcccg acaatctgcc gttagacaat tgaccgcgcc cctcatccaa aggcagcgca
     3001 aaggcgagtg aacgatggcc gaggagattg ccgcaacatc gctgcttcag cacgcgatcg
     3061 acccaacccg cgatcatatc cgcggcaaca aacggccgga cagcgtcagc attgtgctct
     3121 atggcgatta tctctgcccc tattgccgcc gtctgcgctt tgtgatcgcg cggctgcgcc
     3181 aggcgttggg agaccgtctc atctatgtct ttcgccattt tcccaatgag cgcgcccatc
     3241 ccggcgcgga agcgatcgct cgcgcgagcg aagcggtcga gagacagggg cggttctggg
     3301 aactgcatga ttggctctat gcccaggagc ccggcgacat cccgggcggc gtcaccgatt
     3361 tcattcgctc cctcggaatc gacatggagc gctatgagcg cgatctcgcg gacagcgcca
     3421 tccagaagcg cgtcgacgag gaccttgccg acggcaagcg caacggtgtg accgcgacgc
     3481 cgaccgtctt catcgatggt tatcggtatg acggcgcctg ggatttctat tccatgctgg
     3541 agacgctgga gcgccccgtc gcggccaggg tccagcgctc ggcacgcgct ttcgccagtc
     3601 tgcccgcatc cggcggcctt gttcttctgc tcgcggcggc cgcggccatt ctgtgcgcga
     3661 acaccgcact cgcgccctat taccggctgt tcatcgattc gaccgtgggc atcggaacgc
     3721 cgggtagcat gctgacgttg acggtcgggg cctggttctc cgaagggctg ctctcgatct
     3781 tcttcctgct tgtgggtctt gagatccggc gcgagatgac cgcgggtgcg ctcagcgaca
     3841 cgcgtgcagc gattctgccg gtgctcgccg ccgtcggcgg cgtgcttgca cccaccgcga
     3901 tctatcttgc gctcaatccc gcatcgacgg cgcgcggctg gtccgtgccg acggcaaccg
     3961 atatcgcgtt cacactcggc atcctggctc tgctgggcaa tcgcatcacg ccgggtctgc
     4021 gcgtgttcgt tgccgctctc gcggtcgtgg acgacatcct ctcggtgctg acgctggcga
     4081 tcttctatcc ccgggccttc gagatttcat ggcttttcac cagcgcgttg acgatcattc
     4141 ttctctacgc gctcaaccgg tggcgcgttt atgcggcctc gccttatgtg ctggtggcgg
     4201 tgctgctggg gatctcgctg cacggcgcag gcgttcacgc cgctctcgcc ggcgttatcc
     4261 tggcgggttt tctgccgacc cggcccgcac ccgagaccgg gcctttgctc gcccaggcgg
     4321 caacggcgct ggcagcgctc gaacatgcgg aaaacgaggc caaggaatcg ggcgagaacc
     4381 ggcgcatcga acaggaaccg atctgggatt gggcaagccg caatctttcc gccgccagcg
     4441 accgcttgtt gtcgcccgcc gaccgcatcg agcgcgcggt gtctccctgg agcgcttatg
     4501 tgatcctgcc gctctttgcc ttctcggcga ccggcgtcgg tctgaacgtc gatcttgccg
     4561 cacccggtgc gctgccgatt ctgctgggcg tgatcctcgg gctggtgatc ggaaagccgc
     4621 tcggcatttc gctggcgtct ctgctcgcca tcaaaacgag gattgcccgc gcgccggagg
     4681 atgtgaccct ccgcaatttc atcggcgcgg cgtgcctctg cggtgtcggc gatacggttg
     4741 cgcttctgat ggccgatcag gcctttccgc aagggccgga tgcggctgtt gccaagatcg
     4801 gtgttctgat cgggtccgtg ctggcggcag gactcggagc cgcgatcctc gcgccgtcgc
     4861 cgccgatcca tgcggagtcg cctctcccgc aaggctgagg ttggcggttg cgccgcaaca
     4921 tgcaacagtg cgttccgtgg atcgggggcg gagatttcat gtcggttgaa ggcgaaatcg
     4981 tcagagaaga cgcgcatgag ctgcatcgta ccctgggccc gtggcaact
//
LOCUS       DASYEG010000137        27715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2507639, whole genome shotgun sequence.
ACCESSION   DASYEG010000137 DASYEG010000000
VERSION     DASYEG010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 27715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27715
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2507639"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /gene="gcvT"
                     /locus_tag="VGM72_06030"
     CDS             complement(<1..810)
                     /gene="gcvT"
                     /locus_tag="VGM72_06030"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEY4264858.1"
                     /translation="MTAPPPSSLQRTPLHALHVSLGAKMVPFAGYEMPVQYPTGIIKE
                     HLHTRAVAGLFDVSHMGQALLDGDDPAHALEQMTPADIAGLKDGQMRYALLLNDAGGI
                     KDDFMAARLAGERALYLVVNAGTKDGDFSYIAARTGRSASLVPQGARALLALQGPLAE
                     AVLSRHAQDIGMLGFMRVARFIVAGAPAIVSRSGYTGEDGFEISLEGADAERVARTLL
                     AEPEVLPIGLGARDSLRLEAGLCLYGHDIDEGTSPIAAGLGWVIGKRRKLAR"
     regulatory      complement(988..1086)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(1147..2946)
                     /gene="argS"
                     /locus_tag="VGM72_06035"
     CDS             complement(1147..2946)
                     /gene="argS"
                     /locus_tag="VGM72_06035"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY4264859.1"
                     /translation="MISLLAELSATAGAAFAAEALPEALGQVQVSDRPDLAQFQCNGA
                     LAAAKLVLRPAEGETGGLAKGNPRAIAEKIAARLKDSGLFEDVGIAGPGFINLKLKDE
                     LLQARLRQLADDPQSVSPGTGQGKRMILDFGGPNIAKPMHVGHLRSCIIGDCLQRLFR
                     ANGWDVTSDVHLGDWGLPMGQLIAELSLRRPELPYFDPADKSPYPEESPVGMEDLERL
                     YPEAAAACKADPARLEAARQATVELQAGRPGYRALWRHFHNVSAQGLMREFGSLGVVF
                     DEWKGESDADPFIPGMIERLKAAGLAEMDQGALIVSVQETTDTAEMPPLILLKSDGAM
                     NYATTDVATIVERVREHDPDLILYVVDQRQHLHFEQVFRAAKKGGLAGRACFEHIGYG
                     TVNGTDGKPFKTRAGGVMKLYDLIAMATDEAMKRLNEQGLAADYPQSERTKIARTVGL
                     AAIKFADLSNNRGSGYVFDLERFTRFEGKTGPYLQYAAVRIKSILRKAQSEGYASTAP
                     IVACEEERRLALQLLIMNDALAGAEAKRAPNLICDFVFTLAQNFSRFYTEHHILSETD
                     EAVRSARLGLCALTLTQIERLLNLLGIEVPERM"
     gene            complement(2943..3551)
                     /locus_tag="VGM72_06040"
     CDS             complement(2943..3551)
                     /locus_tag="VGM72_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264860.1"
                     /translation="MHRLPVFPLLLLALPLAGCETLGLTTPMPDFSAVDMGPICPATA
                     VLSDAVTVTKLKAGTLPAQPNPANVAFTAEMSQAKLDCNFNREQNRLTVDINFAVKAT
                     RGPAAAGSDPQLDFFVAVIDVDNNIVSKQVYHGQPEMSGHASNVYTQNVSNFPVPMAM
                     DKRPYDYEILTGFQLTPDELAYNRIPRSVPAAATTAPRAANR"
     gene            3641..4615
                     /gene="ispH"
                     /locus_tag="VGM72_06045"
                     /gene_synonym="lytB"
     CDS             3641..4615
                     /gene="ispH"
                     /locus_tag="VGM72_06045"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002190.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY4264861.1"
                     /translation="MNATSQGPLAEKPKLTLLLAAPRGFCAGVDRAIRIVELALEKYG
                     KPIYVRHEIVHNRYVVERLEKIGAIFIEELEEAPCGACVIFSAHGVPKAVPVEAKRRE
                     LFYLDATCPLVSKVHMEAERHHASGRTILLVGHAGHPEVVGTMGQLPPGAVILVETAG
                     DAENLEPPKDTSLAYITQTTLSVDDTAEIVAVLKRRFPDIAGPHKDDICYATTNRQQA
                     IKAVARRCDALFVVGSANSSNSMRLVEVARRAGAHCATLIENAAVIDWNSLVNAKTVG
                     LSAGASAPEILVDEVIAAFHARFDLIVEEAAVTREDIAFKLPRALAGA"
     gene            4619..5578
                     /gene="thrB"
                     /locus_tag="VGM72_06050"
     CDS             4619..5578
                     /gene="thrB"
                     /locus_tag="VGM72_06050"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF003558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEY4264862.1"
                     /translation="MAVYTDVSFEELESFLAAYDLGEPHVFKGIAEGVSNSNYFLQTE
                     KGTYILTLYEMRVETSDLPFYLGLMGHLAGAGLRCPTPVRAKDGAVSGLLNGRAAAIL
                     TFLDGISLRRPTAEHCQQAGLALAELHQAGASFSGRRDNALGPKGWRLLADDCIAQAD
                     SVADGLRVLIEEELTALEAAWPLRLPQGVIHADLFPDNVLFMNDRVSGIIDFYFACND
                     ALAYDLAVALNAWCFENDGAFNVTKGRALIAGYQAKRALQAEEKTAFPLLCRGAALRF
                     LLTRTYDWINRNPAALVRPKDPREFAKRLRFHRNVKSAEAYGL"
     gene            5575..6006
                     /gene="rnhA"
                     /locus_tag="VGM72_06055"
     CDS             5575..6006
                     /gene="rnhA"
                     /locus_tag="VGM72_06055"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEY4264863.1"
                     /translation="MSTVEIFTDGACSGNPGPGGWGAILRSGAHEKEIFGGERLTTNN
                     RMELMAAIRALEALKRASTVIIYTDSRYVMDGLTKWLPRWKANGWKTTDKKPVKNDDL
                     WRALDEAVTRHDVAWRWVRGHDGHLENERADALARGAIPKT"
     gene            complement(6007..6705)
                     /locus_tag="VGM72_06060"
     CDS             complement(6007..6705)
                     /locus_tag="VGM72_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264864.1"
                     /translation="MPQFTFGPIYCETLSATSTGFPVEPMNTLSNGVIVLFGLLSFYL
                     TAKRSPRSLDLYILAALLVTTGVGSGIWHGFRDRDALFFEVQSGLFFLFAFAFFWARR
                     LWSYAGALLFLALFYGGFILSREFWDTTMFGIPIQRWVALAPVVIVAGIALTAQTYAI
                     SKRAAAYGGGALCLAIIALVFRTIDLSVCSAIPIGTHFLWHSFLSAAGCLGVLALIAL
                     PARSVRLVAAAAAE"
     gene            complement(6786..7265)
                     /locus_tag="VGM72_06065"
     CDS             complement(6786..7265)
                     /locus_tag="VGM72_06065"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY4264865.1"
                     /translation="MIKIGDRIPSVNIRQVTESGAQEVNTTDFFKGKRVALFAVPGAF
                     TSVCSAKHLPSFATNADAFAKRGVDVIACISVNDPAVMRAWGEHSDTTGKVSMLADGN
                     GDFARALGAEADMTKNGMGKRSRRYSMLVEDGVVQQFNLEEPGAFGASSGDRLLTQL"
     gene            7388..7981
                     /locus_tag="VGM72_06070"
     CDS             7388..7981
                     /locus_tag="VGM72_06070"
                     /inference="COORDINATES: protein motif:HMM:NF001268.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY4264866.1"
                     /translation="MASSRDQRVDEGESFLEGKLLIALPGMGDERFAQTVIYMCAHSA
                     KGAMGIVINKPIPGLTFAEVMKQLEIETQPLAGELPILYGGPVETGRGFVLHSGEYEA
                     SDSTLPVSEDISLTATLDIVRAMAEGRGPKHALFALGYAGWAPGQVESEFQTNGWLHC
                     PSDPSLVFGVSAEAKWLTALQRLGIGPSGLVSDTGRA"
     gene            complement(7989..10826)
                     /locus_tag="VGM72_06075"
     CDS             complement(7989..10826)
                     /locus_tag="VGM72_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HEY4264867.1"
                     /translation="MIFRALLFALLLFAPYARADEAPRAGRALDFAVVPPVIDLGPSV
                     QPRQRKDAADPNGEWFTLTVQNRSRQPVARVLTAAEPPVALVLAPPVGRPTLIEAAAP
                     DRAILIERAVAFGANAFRVLVPPGRSANLALHLENVAGKPSLLAWTEPALIAHNRQAA
                     VLSGLVSGLLTAAMAFAAGAAAISSRIFPRWAALFLFAVLIAELTIIGVFDGRFLTAM
                     SGPYALFSLALALALAAAIRLVDYVAPFEAFHRDAVRRRDVAALAILLMGVAAYSGIP
                     GTGVAIRVIALIGAAAAAGYLAHCGRIGIAAARRLAPAATIFALVTAVAALNALGFFG
                     VNLVAASAIGGFAAAGTLLVALATAVPVEHSIERLREFEEAHGHDDVHALVTDEAYDQ
                     VRDLAAVAASRQGVFDFDFESSLLSLSAEAGSLLGLPAGVIELSRQSWLEHIHPDDRF
                     VFEKALESYRHHPGMAFRVEFRVNASGHTLWCELRATMTGQAVPAERCLGLIADVTAR
                     KNLDTEHARDLVDSLTGLGNRLALQARLDTIGSDLERFALGVFDLDRFKTVNESLGRD
                     GADVALAAFVERLEHCFAKERHSGHLWLFRVGGDMFAALARDVGDLGNFGQNVLAAMT
                     ASFDIADRDVYLTASVGVAAGGDADDGQDLLAQAELAMIDAKRQGGAKAVLYSGRSQI
                     AIPRDAVALEADLRRALTRGEIEIHYQPIVRLAEREVAGFEALLRWRHPERGLIEPEA
                     FVAHAEETGLILPLGRFALERAIRDLAQWQRLFPGQPPIFVSVNVAWRQISDPAFVGD
                     FASLLAAADIAKRSLKLEITESAVMRDASLAEAALRRLCDLGIGLAVDDFGTGHSSLS
                     HLRRFPFDAIKIDKSFIADAQDNSGAAILRSIVSLAHELKLSVVAEGLETEAEALLLA
                     GMGCEYGQGFLFGSALPGAVIADFIAATRAA"
     gene            complement(10853..11437)
                     /locus_tag="VGM72_06080"
     CDS             complement(10853..11437)
                     /locus_tag="VGM72_06080"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY4264868.1"
                     /translation="MRGMIFPGGREPVLQGDGVVLRYPQMSDYAQWAQLRGESRDFLS
                     AWEPVWAPDELTRAAFRRRLRRYQREIRSDLAYPFLVIRKADDALMGGCTLSNVRRGV
                     TQSAAIGYWIGARFARQGHMYATLRAIRPFAFELLGLHRLEAACIPENVASRNLLLKV
                     GFREEGRARRYLQINGEWRDHILFALLEDDAPIG"
     gene            complement(11447..12733)
                     /locus_tag="VGM72_06085"
     CDS             complement(11447..12733)
                     /locus_tag="VGM72_06085"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY4264869.1"
                     /translation="MSVEITRLPSGLIVATDPMPHLMSAALGVWVNCGARHETPAESG
                     LSHMLEHMAFKGTATRTAKDIATEIEAVGGDINAYTSREQTAFHARVLKDDVGLALDM
                     IADILIYSSFDEGELEREREVIIQEIGQTRDTPDDLIFDYLQEASYPDQPMGWPIFGS
                     EKTVAGFSRGDLVSFMGRHYRAGAMMLIASGAVEHEAMVEAARRLFSPVAEGTERESL
                     PAEFRGGDMRTLDDLEQAHLAFAFPGVASADPDAITAQVFATALGGGMSSRLFQEARE
                     KRGLCYSIYAFSHSYRDTGTIGIYSGTAEDKCGEVAPLIAAEIEAMATGATEEEASRA
                     RAQLKASLLMGLESPHQRCELMAGHLYAYGRVLGLDEVIARVDAVDAGALRRFAERLC
                     RTGNPAVAAVGPVKRLESRDDFARRFGRDLSLTGAN"
     gene            complement(12730..14118)
                     /gene="thrC"
                     /locus_tag="VGM72_06090"
     CDS             complement(12730..14118)
                     /gene="thrC"
                     /locus_tag="VGM72_06090"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY4264870.1"
                     /translation="MSTRGEAGAAFADVLLGGPAADGGLYLPEHWPQFAQSTIDEFAG
                     LPYAEVAFRLMRPFIGDAFSHEEFHADVQSAYAGFAHKAVAPLVQLAPDLFLLELFHG
                     ATLAFKDVAMQLMGRLFARALAKRNRRATVVVATSGDTGSAAIAALRGAAGVDVVVLH
                     PKGRVSEVQRRQMTSVLDSNIHNIALEGSFDDTQTIVKALFADADFAARTNLTAVNSI
                     NFARIVSQCVYYFTAAAALGANAARRVNFVVPTGNFGDIFAGEAAQRMGLAIGRLVIA
                     TNANDILARALASGRYETGHAAPTLSPSMDIQVASNFERALFEASGRDARWVGDAMRG
                     FAQTRSLTIPPAVLQALQARYLAASADDAKTIATIARIYKESGLVIDPHTAIGLAAAE
                     TLEGELRGPTIALATAHPAKFSAAVTEAIGKPAELPPPFGDLLTREERYTVLPNTTHA
                     VRDFVLEHTRQK"
     gene            complement(14140..14844)
                     /locus_tag="VGM72_06095"
     CDS             complement(14140..14844)
                     /locus_tag="VGM72_06095"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEY4264871.1"
                     /translation="MIGFRPQLFPTLWFLPGFALLIGLGIWQIERLHEKVALIASVES
                     GMQAAPVPLDSALREGGAAAEYHHVQVSGRFLHDSELYVFSRGPMGSVGVDVITPLVR
                     ENGDIVLVDRGFVPEALRDPKMRVAGQAAGEVSLTGVLRLSQQPGIFTPAPNRQTRLW
                     FVKDVPSMVAFLNVTAPPILIEADAAPNPGGWPLGGRTQVEFPNDHLQYAITWFGLAL
                     ALLAVYLLYHRKREPG"
     gene            complement(14841..15218)
                     /locus_tag="VGM72_06100"
     CDS             complement(14841..15218)
                     /locus_tag="VGM72_06100"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEY4264872.1"
                     /translation="MSRPNPGAEPSLVYASVLGRCPACGKGKLFRGFLRIASACDTCG
                     LDYSKLEAGDGPAIFVILIVGAIVAGGALLTEVKYSPPYWVHALIWGPAVVILSLGLL
                     RPLKAGLIVLQYKNRAEEGRLVK"
     gene            complement(15215..16138)
                     /locus_tag="VGM72_06105"
     CDS             complement(15215..16138)
                     /locus_tag="VGM72_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY4264873.1"
                     /translation="MAHGDVHHDYHLVDPSPWPIVGSFSAFVMLLGAVFWMNTGANFF
                     GLPIAGQPWIMIIGFVLVLYTMAGWWRDVVKESVVKGDHTPVVKLGLRYGMILFIASE
                     VMFFFAWFWAFFNSALFSLDVGIGHWPPEGIVPFDPWHLPLLNTLILLTSGATVTWAH
                     HAILKSDRKGAIQGLLVTIALGASFTCVQAYEYTHAAFTFGFNHAQMMPFMDVAHKSL
                     TASATGGMGNLNAIYGSTFFMATGFHGLHVIIGTIFLTVCLFRAIAGHFTPGRHFGFE
                     AAAWYWHFVDVVWLFLFACIYVWSSWGVMTE"
     gene            complement(16159..16740)
                     /locus_tag="VGM72_06110"
     CDS             complement(16159..16740)
                     /locus_tag="VGM72_06110"
                     /inference="COORDINATES: protein motif:HMM:NF003465.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY4264874.1"
                     /translation="MKITRNLAVFGCCFGVVIGMVAMSYAAVPLYYAFCRATGYGGTP
                     RRADAAPGAVGTREFTVRFDTNIDPALPWSFLPEQRAVNVKPGEQKLVYFHAVNRSDK
                     PIVGHATFNVTPDDTARYFNKIQCFCFTEQRLEPGESVEMPVIFFVSPTILKNHDEDT
                     VTAITLSYTFYPATNQTTDAKKTAAVPAKGSGG"
     gene            complement(16756..16917)
                     /locus_tag="VGM72_06115"
     CDS             complement(16756..16917)
                     /locus_tag="VGM72_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264875.1"
                     /translation="MNGDDDEARARFAKERFERARRMRNYAIAGTLLFLVVLFFIMTM
                     VRLGGHVAD"
     gene            complement(16914..17861)
                     /locus_tag="VGM72_06120"
     CDS             complement(16914..17861)
                     /locus_tag="VGM72_06120"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEY4264876.1"
                     /translation="MSVVAERPESIVHAELNFASVSDYFALLKPRVMSLVLFTGIVGL
                     VLAPGHLDMATAITALLCIAVGAGASGALNMAYDSDIDALMSRTMGRPVPQGHLTAGT
                     AAAFGATLSIGAVTVMGLLVNLVAAALLAFTILFYVLVYTMWLKRRTPQNIVIGGLAG
                     ALPPAIGWAAATGSLAVPPLVLVAIIFLWTPPHFWALSLYRAEDYARARVPMLPVVSG
                     KAETRRQIVLYTALVVASGFAPTILGFAGPLYLAANVGLGLWFLIQSVRVARERDIVR
                     EPQARRLFGVSIAYLFALFAALLIEQVAGWSHLHTLAVG"
     gene            complement(17873..19504)
                     /gene="ctaD"
                     /locus_tag="VGM72_06125"
     CDS             complement(17873..19504)
                     /gene="ctaD"
                     /locus_tag="VGM72_06125"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEY4264877.1"
                     /translation="MAGAHDTHGAAVHTGHESHPHGWRRYVYSTNHKDIGTMYLVFAI
                     CAGLIGGFLSVMMRAELMYPGMQIFKDPHTFNVFVTGHGLIMIFFMIMPAMIGGFGNW
                     FTPLMIGAPDMAFPRMNNISFWLLPFSFTLLILSMFVEGDSGGEGVGGGWTIYAPLST
                     YGSPGPAMDLAIFALHIAGASSILGAINFITTIFNMRAPGMTLHKMPLFVWSILVTTF
                     LLLLSLPVLAGAITMLLTDRHFGTTFFNPAGGGDPILYQHLFWFFGHPEVYILILPGF
                     GMVSQVVSTFAKKPVFGYLGMAYAMVAIGFIGFVVWAHHMYTVGLTVDTKAYFVFATM
                     VIAVPTGVKIFSWIATMWGGSISFRTPMLWAVGFIFLFTVGGVTGVVLANAGVDVVLQ
                     DTYYVVAHFHYVLSLGAVFAIFSGWYYWFPKITGYMYNETLGKLHFWVMFVGVNLTFF
                     PMHFVGLAGMPRRIADYPDAFAGWNYVASIGSFISAASVLVFLLCMAEAIMKKRVAGD
                     NPWGAGATTLEWTLSSPPPFHTFNDLPRITDGGHH"
     gene            complement(19524..20357)
                     /gene="coxB"
                     /locus_tag="VGM72_06130"
     CDS             complement(19524..20357)
                     /gene="coxB"
                     /locus_tag="VGM72_06130"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY4264878.1"
                     /translation="MIRKLGAFTALFTTAMLSFAVSASAQPVPWQIGLQPAADERMRD
                     IIGLHTFVLYIITAVSLFVLALLLWIALRFNAKANPTPSATTHNTTLEVLWTVVPVLI
                     LVAIAIPSFRLLYFEDVIPPADMTVKAIGKQWFWTYEYPDNGNFTFDAQIVADRRSQP
                     VVPNGPPRMLGTNNHVVVPVGKTVRLITTGADVIHSWALPAFGVKIDAVPGRLNQSWF
                     KADKEGTYYGQCSELCGARHAYMPITVDVVSQEKFDMWVAEAKTKFAAVGAPTRVAVA
                     H"
     gene            20478..22040
                     /gene="tldD"
                     /locus_tag="VGM72_06135"
     CDS             20478..22040
                     /gene="tldD"
                     /locus_tag="VGM72_06135"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF008006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEY4264879.1"
                     /translation="MPRRAVIPLIPLELSSHILDPGDGRAAHAYIVWTGNLSGNPNPS
                     KPMTSHEDLFFTQTGMERRRVQGLIDNTLHSADDGELFLEYRQTESFSFDDGRLKAAT
                     YDTTQGFGLRAVAGEATGYAHASELSEAAIARAAEAVGVVKLGHSGTLAGGPARTNLH
                     LYTDNSPLDSASFDIKVKLMAEIDAYARSADPRVKQVSCSLAGEWQVVEIMRPGGESY
                     RDVRPLVRLSISVVVEENGRQETGYYGGGGRRAYTDYIAPGYWRAGVDEALRSALVNL
                     RSVPAPAGEMSVVLGPGWPGILLHEAVGHGLEGDFNRKKTSAFAGLLGQRVASPGVTV
                     VDDGTISERRGSLTIDDEGTPTSRTVLIEDGILKGFMQDRMNARLMGVAPTGNGRRES
                     FGSQVMPRMTNTYMLSGHQAPQEILASVKNGIYAVQFGGGQVDITSGKFVFSCTEAYR
                     IEDGKIGAPIKGATLIGNGPDAMTRVSMIGNDMKLDTGIGTCGKNGQSVPVGVGQPTL
                     RIDGLTVGGTGG"
     gene            complement(22045..22734)
                     /locus_tag="VGM72_06140"
     CDS             complement(22045..22734)
                     /locus_tag="VGM72_06140"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY4264880.1"
                     /translation="MFSVLFEVHPKADRWDAYLDTARMLKPELEQIEGFADNIRYRSL
                     TREGWLLSLSSWRDEKSVVRWRTVARHRQAQEKGRSEIFADYHLRVGEITRDTRPPAG
                     CVLREQRLDETEIGEGTIVTLIDGKRPPEWVKQARSEDAATWLGLATQAPGLVAWDVF
                     EAILTPGDIILMSSWRDVSAAERFERGVSLPDGARLRNVRVVRDYGMFDRREAPQYYP
                     EVKRASAPEAR"
     gene            complement(22743..23279)
                     /gene="msrA"
                     /locus_tag="VGM72_06145"
     CDS             complement(22743..23279)
                     /gene="msrA"
                     /locus_tag="VGM72_06145"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY4264881.1"
                     /translation="MAASTERAVLAGGCFWGMQDLLRRYPGVVSTRVGYSGGDVANAT
                     YRNHGNHAEAIEIVFDPQKISYRKLLEFFFQIHDPTTLNRQGNDRGASYRSAIFYTSE
                     GQKETALDTIADVEASGLWPGKVVTEVTPASDFWEAEREHQDYLERYPTGYTCHFVRP
                     NWVLPKRNSQNAHAEARQ"
     gene            23525..26239
                     /gene="ppc"
                     /locus_tag="VGM72_06150"
     CDS             23525..26239
                     /gene="ppc"
                     /locus_tag="VGM72_06150"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF000584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEY4264882.1"
                     /translation="MAERHLAIVSSLAPGTASRESVRLLGRQLGEIIREQHGQAAFDR
                     VENLRRHVVHEHREGRSAVALLRQFSHLPNRDLTILIRSFAIFSQLANIADDYIARCE
                     VESDDCEDSLEALRSHPKMTADRVYAYLNGATVAPVITAHPTEVRRKSVIDRESAIAD
                     LLPAYHHFAPGKHRRAEIETQIKREIRMLWQTRMLRPERIRVTDEIDYAASILGHTFA
                     SQLPVVKRKLAHVFGLQGPLPRSLRVGSWVGGDRDGNPFVTAQTLDYSVRHLAGIVFD
                     HYLEQLHLLGAELSLSDELAPVSKALTALAANAPHASLHKADEPYRQALTYSYARLSS
                     TRKILLGHGAMRPPRTEAEPYRNVYELSDELAVVANSLIHNGSADLADGRLAELREAV
                     ESFGFHFAVMDLRQNSAVHERVVGELLREAGVSQDYAELAEDARVALLLKELDNPRLL
                     RTLYRTYSEETAKELDIVDMTARLRRQFGESAVENYVISMAKSVSDILEVAILLKEAG
                     LFIPGAKPQASLRIVPLFETIEDLRDSARIMEAYFTLPLARSILAAQHDLQEVMIGYS
                     DSNKDGGYVTSNWEIRAAIVRLIALAKSRGLRLRFFHGRGGSVGRGGGPSFEATRAFP
                     AGAVEGGIRVTEQGEVVASKYGHPDVGRQTLERMVAATLLADIDPETDAADGALAESF
                     AIFSAEAYRAYRALVYETPRFDLYFRQSTPLPEISDLKIGSRPASRKNSTRIEDLRAI
                     PWVFSWSQARLTLPGWYGFGAAAAKMQEMGRDAELTALYAQSRFFRTVVSNLEMVLAK
                     SNLDIARHYADLVEDQTLANAIFARIEAEHRATLDTVLALTGQKTLLENNPTLAESIR
                     LRLPYIDALNLLQLDLLRRRRSGKEDPETVHAIHMSINGVSAGLRNSG"
     gene            26278..26556
                     /locus_tag="VGM72_06155"
     CDS             26278..26556
                     /locus_tag="VGM72_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264883.1"
                     /translation="MNQYWFRPKQRGIGAGVPLNWKGWALFATYVVAILAVPSLYELY
                     LGYPGTPLLRVLGIVAVSVPFLILAWKKTEGGWRWRRGAEDADDGNPA"
     gene            complement(26571..>27715)
                     /locus_tag="VGM72_06160"
     CDS             complement(26571..>27715)
                     /locus_tag="VGM72_06160"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEY4264884.1"
                     /translation="EGENIIGLSQNGAALSLEPGGQFELSGAPLKTVHETCVEVNTHL
                     KQVREVCADIEAGCLGVGYAPTWRLDDVPVMPKGRYAIMRRYMPKVGGYGLEMMFRTC
                     TVQGNFDFGSEADMVKKFRVALALQPVATALFANSPFREGKANGFLSLRSQIWTDVDN
                     DRAGMLPFVFEDGFGFERYVDYALDVPMYFVYRDGRYIDVAGKSFRDFLTGRIAELKG
                     ITPMMSDWADHLTTIFPEARLKSYLEVRGADGGTWRRICGLPALWAGIFYDSTALDVA
                     WDLVKDWTAEERESMRATVPKLAFKTPFRKTNLLELARRMLELAAHGLQARGALDSSG
                     GTEEGFLQPLQELVERGQTRADELLALYRGEWKGDLGKLFEAYNFL"
BASE COUNT         5594 a         8714 c         8547 g         4860 t
ORIGIN      
        1 gcgcgccagc tttcgcctct tgccgatcac ccagccgaga cccgcggcaa tcggtgaggt
       61 gccctcgtcg atatcgtggc cgtaaaggca aagcccggcc tccagccgca atgaatcccg
      121 cgcgccaaga ccgatcggga ggacttcggg ttcggccagg agagtgcgcg ctacgcgttc
      181 cgcatccgca ccctccagcg aaatctcgaa accgtcctcg cccgtatagc ctgaccggct
      241 gacgatcgcg ggcgcaccgg ccacaataaa gcgtgcaacc cgcatgaaac cgagcatgcc
      301 aatatcttgc gcgtgacggg acagcacggc ttccgcaagt ggaccttgta acgccagcaa
      361 cgcgcgcgcg ccttgcggaa cgagcgaggc gcttctgccc gtgcgcgcgg cgatatagga
      421 aaaatcgccg tcctttgtgc cggcattgac cacgagatag agcgcacgct cccccgcaag
      481 ccgcgccgcc atgaaatcgt ccttgatgcc gccggcatcg ttcagcagca gagcgtagcg
      541 catctgccca tctttcaggc ccgcgatgtc tgcgggggtc atctgttcca gcgcatgggc
      601 cggatcgtcg ccgtcgagca acgcctgccc catatgggag acgtcgaaca gacccgctac
      661 cgcacgcgta tgcagatgtt ccttgatgat gccggtggga tattgcacag gcatctcata
      721 gccggcgaag ggcaccatct tcgcccccag cgacacatgc agcgcgtgaa gcggggttcg
      781 ttgcaacgag gacggcggcg gcgcggtcat ggccagagac tcatgggcga tcactcatgg
      841 caatccgatt ccatacgggt tcgcgttgtt ccgagcctta tcggaaaaaa caacacaccc
      901 cgtctgtcct tggacctgag agaatgaccg aagatcttcg gcttacccct tcggtgaggg
      961 ctgctgccct tctatccaga ctgtctttcc cccgacgcgg tccttttgcc tgagagtttc
     1021 cggggcggtt gctccttcgg cgcctgtctt cagcgtacga agacaaatct ctcccgcgac
     1081 tgggatgcga ttgcccacga tattaggcgc gagggccttc aagtcaatcg cacgctttgc
     1141 ctgccgctac atccgttccg gaacttcgat tcccagaaga ttcagcaggc gctcgatctg
     1201 cgtcagcgtc agcgcgcaaa gcccaagccg ggcggaacgt accgcctcgt cggtttcgga
     1261 caggatgtga tgctcggtat agaagcggct gaaattctgc gccagcgtga acacgaagtc
     1321 gcagatcaga ttcggcgcgc gcttcgcttc cgcgcccgct agcgcatcgt tcatgatgag
     1381 cagttgcagc gcgaggcgac gctcctcttc gcaagcgaca atcggggccg tgctcgcata
     1441 gccttcgctc tgcgccttgc gcaagatgga tttgatgcgc accgccgcat attgcagata
     1501 gggtccggtc ttgccttcga agcgcgtgaa ccgttcaaga tcgaagacat agcccgaacc
     1561 gcgattattc gacagatccg cgaatttgat cgccgccagg cccaccgtcc tggcgatttt
     1621 ggtgcgctcg ctttgcgggt aatcggccgc cagaccctgc tcgttcaggc gcttcatcgc
     1681 ctcatccgtc gccatcgcga tcaggtcgta aagcttcatc acaccgccgg cgcgcgtctt
     1741 gaagggcttg ccgtccgttc cgttcaccgt gccatagccg atatgttcga agcacgcgcg
     1801 gcccgcgaga ccgcccttct tcgccgcacg aaagacctgc tcgaaatgca gatgctgccg
     1861 ctgatccacg acatagagaa tgaggtcggg atcgtgttcg cgcacccgct ccacaatggt
     1921 ggcgacatcg gtggtggcat agttcatcgc accatccgat ttcaaaagga tcagcggcgg
     1981 catctccgcg gtatcggtgg tctcctgcac ggacacgatc aacgcgccct gatccatttc
     2041 ggcgaggccc gcggccttca gcctctcgat catccctggg atgaacggat cggcgtcgga
     2101 ttcgcctttc cattcgtcga atacgacacc caggctgccg aattcgcgca tcagaccttg
     2161 ggcggagacg ttgtggaaat gccgccacag cgcgcggtag cctggccgtc cggcttgtag
     2221 ctcgacggtg gcctggcgcg cggcttcgag ccgggccggg tcggccttgc acgccgccgc
     2281 ggcttcggga tagagccgtt ccagatcttc catgccgacg ggcgattcct ccgggtacgg
     2341 acttttgtcg gcgggatcga aatagggaag ctcgggccgt ctgaggctga gctccgcgat
     2401 caattggccc atcggcagac cccaatcgcc gagatgaacg tcgctcgtga cgtcccagcc
     2461 gttggcgcgg aacagccgtt gcagacaatc cccgatgatg caggagcgca aatgaccgac
     2521 atgcatgggc ttggcgatat tgggaccgcc aaaatccagg atcatgcgct tgccttggcc
     2581 agtccccggg ctcacggatt gcggatcgtc cgcaagttga cgcagccgcg cctgcagcaa
     2641 ttcatccttc agcttcagat tgatgaagcc gggcccggcg attcccacgt cctcgaacag
     2701 gccgctgtcc ttcagccgcg cggcgatttt ctcggcgatg gcgcgcggat tgcctttggc
     2761 cagccccccg gtctcgcctt cggcgggccg taggacaagc ttggccgcgg caagcgcgcc
     2821 attgcactgg aattgcgcaa ggtctggccg gtccgagacc tgaacctgtc ccaaggcttc
     2881 aggcaaggct tcggccgcga acgcagcacc tgcggtcgcg gaaagttcgg caagaagcga
     2941 aatcatcggt ttgcggcgcg cggcgcggtg gtggcggcgg caggcacgga cctcggaatg
     3001 cgattgtagg cgagctcgtc cggtgtgagc tggaacccgg tcaggatttc gtaatcatag
     3061 ggccgcttat ccatcgccat aggcacgggg aaattgctca cgttctgggt ataaacattg
     3121 ctcgcgtgcc cgctcatttc aggctgaccg tgatagactt gcttgctgac gatattgtta
     3181 tcgacatcga tcaccgcgac gaagaaatcg agctgcgggt cggatcccgc cgccgccggc
     3241 ccgcgtgtgg ccttgacggc gaaattgatg tccacggtta ggcggttctg ttcccggttg
     3301 aaattgcagt cgagcttcgc ctgcgacatt tccgccgtga acgcgacatt ggccggattg
     3361 ggctgggcgg gcagcgtacc cgccttaagc ttcgtcacag tcacggcatc cgacaacacc
     3421 gcggtcgcgg ggcaaatcgg ccccatgtcc acggctgaaa aatcgggcat cggggtggta
     3481 agacccagcg tttcgcagcc tgcgagcggc agggcgagca gcaaaagcgg aaaaacgggc
     3541 aagcggtgca tcgaatattc cattccatag cccttatcaa agggcgcggc ggactgtaac
     3601 caagcagggc acggaagaaa agccgcgcta tgatgcaaac atgaatgcga catcccaggg
     3661 gccgcttgcc gagaagccga aattgacgct gctgctcgcg gcgccgcgcg gcttttgtgc
     3721 cggtgtggat cgtgcgatcc gcatcgtgga actggcgctc gagaaatatg gcaagccgat
     3781 ctatgtgcgc catgaaatcg tccacaatcg ttacgtggtc gagcggctgg agaaaatagg
     3841 agccatcttc atcgaagagc tggaagaggc gccctgcggc gcgtgtgtga tcttctccgc
     3901 ccatggcgtg cccaaagcgg tcccggtcga ggccaaacgg cgggagctgt tttacctgga
     3961 cgccacctgt ccgctggttt ccaaagtaca catggaagcc gaacgccatc atgcgagcgg
     4021 ccgcacgatt ctcctggtcg gccatgccgg tcatcccgag gtggtcggca ccatggggca
     4081 attgccgccg ggcgctgtga tccttgtgga gaccgccggc gacgcggaaa atctggaacc
     4141 acccaaggac acgagccttg cctatattac ccagaccacg ctttcggtcg acgacacggc
     4201 cgaaatcgtc gcggtgctca agcggcgttt cccggacatc gcgggaccgc acaaggacga
     4261 catctgctat gccaccacca atcgccagca ggcgatcaag gcggtggcgc gccgttgcga
     4321 tgccctgttc gtcgtcggct cggccaattc gtcgaactcc atgcgcctcg tcgaagtggc
     4381 gcgccgtgcc ggggcccatt gcgcgacgct gatcgaaaac gccgccgtga tcgattggaa
     4441 ttcgctcgtc aacgccaaga ccgtcggctt gtcggcgggg gcgtccgcac cggagattct
     4501 ggtcgacgag gtgattgcgg cgttccatgc gcgtttcgac ctcatcgtcg aggaagcggc
     4561 ggtgacgcgc gaggatatcg cgttcaagct tccgcgcgca cttgcgggcg cctgatccat
     4621 ggccgtctat accgatgtct cgttcgaaga gctggagagc ttcctcgcgg cctatgacct
     4681 gggcgaacct cacgtgttca agggaatcgc cgagggcgtc tccaattcca attatttcct
     4741 gcaaacggaa aaaggcacct acatcctcac gctgtatgaa atgcgcgtgg aaacctccga
     4801 cctgcccttc tatctcggcc tgatgggaca tctggcgggc gccggtcttc gctgtcccac
     4861 gccggtcagg gcaaaagacg gcgcggtctc tggtctcctg aacgggcgcg cagccgccat
     4921 tctcacattc ctcgacggca tctcgttgcg acggccgacg gcggagcatt gccagcaggc
     4981 gggtcttgcg cttgccgagc tgcatcaggc cggagcgagc ttttccggac gccgcgacaa
     5041 cgcgctcggc cccaagggct ggcgcttgct tgccgatgat tgcatagcgc aggccgatag
     5101 tgtggccgac ggattgcgcg ttctcatcga agaggaattg accgcgctcg aagccgcctg
     5161 gcccttgcgg ctgccgcaag gcgtgatcca cgccgatctc tttcccgaca atgtgctgtt
     5221 catgaacgac cgcgtttccg gcatcatcga tttctatttc gcctgcaacg acgcactcgc
     5281 ctacgatctt gcggtggcgc tgaacgcgtg gtgcttcgag aatgacggcg cattcaacgt
     5341 caccaaaggc cgcgcgctga tcgcgggcta tcaagccaag cgcgcgcttc aagccgagga
     5401 gaaaaccgcc tttccgctgc tttgccgagg tgccgcgctg cgtttcctcc tgacgcgcac
     5461 ctatgattgg atcaatcgca atcccgcggc gctggtcaga cccaaggacc cgcgcgaatt
     5521 cgccaaacgt ctgcgctttc atcgcaacgt caaaagcgcg gaggcctacg gcctgtgagc
     5581 acggttgaaa tcttcacgga tggcgcatgc agcggcaatc cggggccggg tggatggggg
     5641 gcgatcctca ggtccggcgc gcatgaaaag gaaattttcg gcggcgagcg cctcaccacc
     5701 aacaaccgca tggagttgat ggccgccatc cgcgcgctcg aggccctgaa acgggccagc
     5761 acggtgatca tctatacgga ttcgcgctat gtcatggatg gcctgaccaa atggctgccg
     5821 cgctggaagg cgaatggctg gaaaaccacc gacaagaaac cggtgaagaa cgacgatctg
     5881 tggcgggcgc tcgatgaagc cgtcacacgc cacgacgtcg cgtggcggtg ggtgcgcggt
     5941 catgacgggc atctggaaaa tgagcgcgcc gatgcgctgg cgcggggcgc cattccgaag
     6001 acgtaattat tccgctgccg ccgcggcgac gagccgtacg ctgcgcgccg gaagcgcgat
     6061 cagcgcaagc acgccgagac agccggccgc ggacaggaac gagtgccaga gaaaatgcgt
     6121 gccgatcggg atggcggaac agaccgaaag atcgatggtg cggaaaacga gcgcgatgat
     6181 cgccaggcaa agcgcgcccc cgccataggc cgcggcacgt ttggaaatgg cataagtctg
     6241 cgcagtgagc gcaattccgg cgacgatgac cacgggtgca agcgccaccc agcgctgaat
     6301 cgggattccg aacatcgtgg tgtcccaaaa ttcgcgcgac agtatgaagc cgccatagaa
     6361 cagcgcgagg aacagcaacg cacccgcata ggaccacagc cgccgcgccc agaagaacgc
     6421 gaaagcaaac aggaagaaca gtcccgactg cacctcgaag aacagcgcat cgcggtcgcg
     6481 aaatccgtgc cagatgccgc tgccgacgcc cgtggtgacg agaagcgcgg ccagaatata
     6541 caggtcgagg gaccggggac tgcgttttgc tgtgagatag aacgagagga ggccgaacag
     6601 cacgatgacg ccgttcgaga gggtgttcat cggctcgacc ggaaatccgg tcgaggtcgc
     6661 cgacagcgtc tcgcaataga tcggcccaaa agtgaattgc ggcatagggg gagcaatgac
     6721 tggggacgcc cgacaataaa gcttttgccg gccgcaggaa accgcccctt aaagcattga
     6781 gggcttcaga gctgcgtcag cagacgatcg ccgctggagg cgccgaaggc acccggttct
     6841 tccagattga actgttggac aacgccatcc tccacgagca tggaatagcg acggctgcgc
     6901 ttgcccatgc cgttcttcgt catatccgct tccgcgccga gcgcgcgcgc gaaatcgcca
     6961 ttgccgtccg caagcatgct cacctttccc gtggtatcgc tgtgctcgcc ccaggcgcgc
     7021 atcaccgccg gatcgttgac ggagatgcag gcaatcacgt caacgcctct tttcgcgaat
     7081 gcgtccgcgt tggtggcaaa gcttggcaga tgcttagccg aacacaccga cgtgaaggcg
     7141 ccgggcacgg cgaacaacgc aacgcgcttg cccttgaaga aatctgtcgt attgacctct
     7201 tgcgcgccgc tctccgtgac ctgacggatg tttacggatg gaatcctgtc tccgatctta
     7261 atcattgctt acgccttcct gtttgctgcg gtaccagctt gcctgggctt gtagctcaca
     7321 agagacgtgt ccggaagacg cgaaaccacc tcttttcggg gagcgcgcgt atcaataagg
     7381 tgacgccatg gcatcttctc gcgaccaacg cgtcgatgaa ggcgagagct tcctggaagg
     7441 caagctcctg attgcgcttc ccggcatggg agacgagcgt tttgcccaga cggtgatcta
     7501 tatgtgcgct cactccgcta agggggccat gggcattgtc atcaacaagc cgatccccgg
     7561 tctgaccttt gccgaagtga tgaaacaatt ggagatcgag acgcagcctt tggcggggga
     7621 acttcccatt ctctatggcg ggccggtcga gaccgggcgc ggcttcgtgc tccacagcgg
     7681 cgaatatgag gcgagcgatt ccaccctgcc ggtgtccgag gatatttcgc tcaccgccac
     7741 gctggatatc gttcgcgcga tggccgaggg gcgcggcccc aagcatgcgc tattcgcgct
     7801 cggttacgcc ggttgggcgc cgggccaggt cgaaagcgaa ttccagacca atggctggct
     7861 tcattgcccg tccgatccgt cgctcgtttt cggcgtcagc gcggaggcca aatggctgac
     7921 cgcgttgcaa cggcttggga tcggcccgtc gggtctcgtc tccgatacgg gccgcgccta
     7981 gagcgatgtc acgcggcgcg cgtggctgcg atgaagtccg cgatgacggc gcccggcagg
     8041 gccgatccga acaggaaacc ctgaccatat tcgcacccca tgccggcgag cagcaaagcc
     8101 tcggcttcgg tctccagacc ttcggccacg acactgagct tcaattcatg ggccagcgac
     8161 acgatcgagc gcagaatcgc cgcaccggaa ttatcctgcg catcggcaat gaagcttttg
     8221 tcgatcttaa tcgcgtcgaa cggaaagcgg cgcagatggc tgagcgagga atggccggtc
     8281 ccgaaatcat cgacggctag ccctatccct aaatcacaga gccgccgcaa cgcggcctcc
     8341 gcgagcgagg cgtcgcgcat cacagcgctc tccgtgattt cgagtttcag ggagcgcttt
     8401 gcgatgtccg ccgcggcgag cagcgatgcg aaatccccca cgaaagccgg gtcggaaatc
     8461 tgccgccagg cgacattcac gctgacgaag atcggcggct gcccgggaaa gaggcgctgc
     8521 cattgcgcca gatcgcggat agcacgttcc agcgcgaaac gtccgagcgg caagatgagt
     8581 cccgtctctt cggcgtgcgc cacgaaggct tccggctcta ttagcccgcg ctccggatga
     8641 cgccagcgga gcagggcttc gaaccccgcc acctctcgct ccgcgaggcg gacaatgggc
     8701 tgataatgga tttcgatctc gccgcgcgtc agggcccggc gcaaatcggc ttcgagcgcc
     8761 accgcatcgc gcggtatggc gatctgcgaa cggccggaat agagcaccgc cttagcgccg
     8821 ccctggcgct tcgcgtcgat catggcaagc tcggcctggg cgagaaggtc ctgaccatca
     8881 tcggcgtcac caccggcagc gaccccgaca ctcgccgtca gataaacatc gcggtcggcg
     8941 atatcgaaag aggccgtcat cgcggcgagc acgttctggc cgaaattccc gagatcgccg
     9001 acgtcccgag ccagggcagc gaacatgtcg ccgcccacac ggaaaagcca caaatggccg
     9061 gaatggcgct ccttcgcgaa gcaatgttcc aagcgctcta cgaaggcggc gagcgccacg
     9121 tccgccccgt cgcgccctag ggattcgttc acggtcttga agcggtcgag atcgaacacg
     9181 ccaagcgcaa atcgttccaa atcggaaccg atggtgtcga gacgcgcctg cagcgcaagg
     9241 cgattgccca gacccgtcag cgaatccacg aggtcccggg cgtgctccgt atccaagttc
     9301 ttgcgcgcgg tcacatccgc gatgaggcca agacagcgtt cggctggcac agcctgtccg
     9361 gtcattgtgg cgcgcagctc gcaccacagc gtatgaccgg aagcgttgac gcgaaactcc
     9421 acccggaacg ccatgccggg gtgatggcgg tatgattcga gtgctttttc gaatacgaaa
     9481 cggtcgtccg gatgaatatg ttccagccag gattggcggg aaagttcgat cacgccagcc
     9541 ggtaaaccaa gcagcgatcc ggcttccgcc gaaagcgaaa gcaagctgct ttcaaaatcg
     9601 aaatcgaaca cgccctggcg ggaggccgcg acagccgcaa gatcgcgcac ctggtcatag
     9661 gcttcgtcgg tgacgagcgc atggacatcg tcatgaccgt gggcctcttc gaattcgcgc
     9721 agacgctcga tggaatgttc gaccggaaca gcggtcgcca gagccaccag cagcgtgccc
     9781 gcagcggcaa aaccgccgat ggcgctcgcc gccaccagat tgacgccaaa aaagccgagc
     9841 gcattgaggg ccgccaccgc cgtaaccaag gcgaatatcg ttgctgccgg tgccagccgg
     9901 cgcgcagccg cgataccgat gcggccgcaa tgggccagat aacccgccgc cgcggcagca
     9961 ccgatgagcg cgatcacccg gattgcgact cccgtgcccg ggatgccgga ataggcggcg
    10021 acacccatga ggaggatcgc aagagccgcc acatcgcgcc ggcgcacggc atcgcgatga
    10081 aacgcctcga acggcgcgac ataatccacc agcctgatcg ccgcggcgag cgccaaagcg
    10141 agcgcgagcg agaacagggc ataggggccg ctcatggcgg tgaggaacct gccgtcgaac
    10201 acaccgatga tggtcagctc ggcaatcagc acggcaaaca gaaacagcgc cgcccagcgt
    10261 gggaatatcc gcgaagaaat tgccgcggcc cccgcggcga acgccatggc cgccgtcagc
    10321 agacccgaaa cgagtccgga aagaaccgcc gcctgccgat tgtgtgcgat cagcgcgggc
    10381 tcggtccagg caagcaatga cggctttccc gccacattct ccaaatgcag cgcgagattc
    10441 gccgaccggc ctggcggtac gaggacgcga aatgcattcg ccccgaacgc gaccgcgcgt
    10501 tcgatcagga tcgcgcgatc cggcgcggcc gcttcgatca aagtgggacg ccccactggc
    10561 ggcgcgagga cgagagcgac cggcggctcg gccgcggtca gcacgcgcgc gaccggctgg
    10621 cgcgatcgat tctgcaccgt aagcgtgaac cattctccgt ttgggtcagc cgcatccttg
    10681 cgttgacgcg gctgcaccga tgggccgagg tcgatcaccg gcggcaccac cgcgaaatcg
    10741 agtgccctgc cggcgcgcgg cgcttcatcg gcgcgcgcat agggcgcaaa caacaggagt
    10801 gcgaaaagca acgcgcggaa gatcaagggg aatagctcgt ttaacgccag cactaaccta
    10861 taggcgcatc atcttccagc aaagcgaaga gaatatggtc gcgccattcg ccgttgatct
    10921 gcagatagcg tcgtgcccgc ccctcttcgc gaaatccaac cttaagcagc agattgcgcg
    10981 aggccacatt ttcggggatg caggccgcct ccagccgatg caatcccagg agctcgaagg
    11041 cgaagggccg gatcgccctc agggtggcgt acatatgccc ctgacgggca aagcgcgcgc
    11101 cgatccagta gccgatcgcc gcggattggg taacgccgcg ccgcacattg ctgagcgtgc
    11161 agccgcccat cagcgcatcg tcggccttgc gaatcaccag aaagggatag gcgagatcgc
    11221 tcctgatctc gcgctgatag cggcgcaggc ggcgccggaa ggcagcccgc gtcagctcat
    11281 cgggcgccca cacgggttcc caggccgaga gaaagtctcg gctttcgcca cgaagctgcg
    11341 cccattgcgc atagtcggac atttggggat agcgcaggac gacaccatcc ccctgcagta
    11401 cgggctcgcg tccgccggga aagatcatgc cgcgcatgaa catggctcag ttcgcacccg
    11461 tgagcgaaag gtcgcggcca aaacggcgcg cgaaatcgtc gcgcgattcg agccgcttca
    11521 ccgggcccac cgcggcaacc gccggattgc cggtccggca gagcctttcg gcgaaacggc
    11581 gaagcgcacc ggcatccacc gcatcgacgc gggcgatcac ctcgtccagt cccagcacac
    11641 ggccataggc gtaaagatga cccgccatca attcgcaacg ctggtgcggg ctttccaacc
    11701 ccatcagcag ggaggctttg agttgcgcgc gcgcacgcga tgcctcttct tccgtcgctc
    11761 ccgtggccat ggcctcgatt tcggcggcga tgagcggggc gacttcgccg catttgtctt
    11821 cggcggtacc ggaataaatc ccgatggtgc cggtgtcgcg ataggagtgc gagaaagcgt
    11881 aaatcgaata acaaaggccg cgcttttccc gcgcctcctg aaacaggcgc gaggacatgc
    11941 cgccgccgag cgcggtggca aacacttgag ccgtaatcgc gtcgggatcc gcggaggcga
    12001 ctcccggaaa agcgaaagcg agatgcgcct gctcgagatc atcgagcgta cgcatatcgc
    12061 cgcctctgaa ctccgcggga agcgattcgc gttcggtccc ttctgccacc ggtgagaaaa
    12121 gcctgcgggc ggcttcgacc atcgcttcgt gctccacggc ccccgaagcg atcagcatca
    12181 tcgcgcccgc gcggtagtgc cttcccatga aggagacgag atcgccgcgg gaaaacccgg
    12241 caacggtttt ttcgctgccg aagatcggcc aacccatcgg ctgatcggga taagatgctt
    12301 cctgcagata atcgaagatg agatcgtcgg gcgtatcgcg ggtctggccg atttcctgga
    12361 tgatgacctc gcgctcgcgt tcgagttcgc cttcgtcgaa gctcgaatag atgaggatgt
    12421 cggcgatcat atcgagggcc aagcccacat catccttgag cacgcgggcg tggaaggcag
    12481 tctgctcgcg gctggtatag gcgttgatat cgccgccgac ggcttcgatc tcggtagcga
    12541 tgtccttggc cgtgcgcgtg gcggtgcctt tgaacgccat atgctcgagc atgtgcgaaa
    12601 gtcctgattc ggcgggtgtc tcgtgccgcg cgccgcaatt cacccacacg ccaagcgccg
    12661 cgctcatcag atgcggcatg ggatcggtgg cgacgatgag accagatggc aggcgggtga
    12721 tttcgacgct catttttgtc ttgtgtgctc caaaacgaaa tcccgaaccg cgtgcgttgt
    12781 gttgggaagg acagtgtacc gctcttcacg ggtcaagaga tcgccgaagg gggggggcag
    12841 ctccgccggt ttgccaatgg cctcagtgac cgcggcggag aattttgcgg gatgcgccgt
    12901 ggcgagcgct atggtgggac cgcgaagctc gccttcaagt gtctcagccg ccgcaaggcc
    12961 tatggcggta tggggatcga tgacaaggcc gctttccttg tagattcgcg cgatcgtcgc
    13021 gatggttttg gcgtcgtccg cgctggccgc gagatagcgc gcttggagcg cctgcagaac
    13081 ggcgggcggg attgtcaggc ttcgcgtttg agcgaaacca cgcatcgcat ccccgaccca
    13141 tcgtgcatcg cgaccggagg cttcgaacag cgcacgttcg aaattgctgg ccacctgaat
    13201 atccatcgag gggctgagcg tcggggccgc atgtccggtt tcatagcgcc cgcttgccag
    13261 cgcgcgtgcc agaatatcgt tggcattggt ggcaatgacg agccggccga tcgcaagacc
    13321 catgcgctgt gcggcttcgc ccgcgaagat gtcgccgaaa ttgcccgtgg ggacgacgaa
    13381 gttgacgcgg cgcgcggcat tcgcgcccag cgctgccgca gcggtaaaat aatagacgca
    13441 ttgcgacacg atgcgcgcga aattgatcga attgaccgcc gtgagatttg tgcgcgccgc
    13501 gaaatcggca tcggcaaaaa gcgccttcac gatggtctgg gtatcgtcga agctgccctc
    13561 aagcgcaatg ttgtggatgt tggaatccag tacgctcgtc atctggcggc gctgcacctc
    13621 cgagacacgc cccttggggt gcagcacgac aacatccaca ccggcggcgc cgcgcaaggc
    13681 ggcgatggcc gccgagccgg tatcgccgga cgttgccacc accaccgtgg cgcggcggtt
    13741 ccgcttggct agcgcgcgcg cgaagagacg ccccatgagc tgcatcgcca cgtccttgaa
    13801 ggcaagcgtc gctccgtgga agagctcaag caggaagaga tcgggtgcga gctggaccag
    13861 cggcgccacc gctttgtgtg cgaaccccgc ataggcgctt tgcacgtcgg cgtggaattc
    13921 ctcgtgggaa aacgcatcgc cgatgaaggg gcgcatgagc cggaatgcga cctccgcata
    13981 cggaagaccc gcgaattcgt cgatggtgga ttgcgcgaat tggggccaat gctcaggcag
    14041 ataaagcccg ccatccgccg ccgggccgcc cagcagcacg tcggcgaacg ccgcccccgc
    14101 ctctcctctt gtgctcacat aatgcatgac gcccctgatt cagcccggct cgcgcttacg
    14161 atgataaagc agataaaccg caagcagcgc caaagcgagc ccgaaccagg tgatagcgta
    14221 ctgcaaatgg tcgttgggga actccacctg cgttcgcccc ccgagcggcc agccgcccgg
    14281 attgggagcc gcatcggctt cgatcaggat gggaggggct gtcacattca ggaaggcgac
    14341 catgctcggc acatccttga cgaaccacag ccgggtctgg cgattggggg cgggggtaaa
    14401 tatcccgggc tgctgcgaga gccgcaacac tcccgtcagc gacacctcgc cagccgcctg
    14461 accggctacc cgcatcttgg gatcgcgcaa ggcttcgggc acgaaccccc ggtcgaccag
    14521 aacgatgtcg ccattctccc gaaccagcgg cgtgatgaca tcgacaccga ccgaccccat
    14581 cggcccacgc gagaaaacat agagttcaga atcgtgcagg aaacgcccgc tcacctggac
    14641 atgatggtat tcagccgcgg cgccaccttc ccgcaatgcg ctgtcgagcg gcacaggagc
    14701 cgcctgcatg ccggattcga cactcgcaat cagcgctacc ttttcgtgta gacgttcgat
    14761 ctgccagatt ccgaggccta tcagcaatgc aaagcccggc aggaaccaga gggtgggaaa
    14821 caattgggga cgaaagccga tcacttcacc agccggcctt cttcggcgcg atttttgtac
    14881 tggagcacga tgagccctgc cttgagcgga cgcagcaagc caagcgaaag gatcaccacc
    14941 gcaggtcccc agatcagcgc atgcacccag tagggtgggc tgtatttaac ctctgtcagc
    15001 agcgcaccgc ccgccacaat cgcgcccacg atcaggatca cgaatatggc aggcccgtcg
    15061 cccgcctcaa gcttcgagta atcgagaccg catgtatcgc aggcgcttgc gatacgcagg
    15121 aagccacgaa acagcttgcc ctttccgcaa gccggacaac gtcccaacac agaggcgtaa
    15181 acaagcgacg gttcagcccc cggatttggg cggctcattc ggtcatcaca ccccaggaac
    15241 tccagacata gatgcaggca aagaggaaca gccacaccac gtcgacgaag tgccaatacc
    15301 aggcggccgc ttcaaaaccg aaatggcgtc ccggggtgaa gtggccggcg atagcccgga
    15361 acagacagac ggtcaggaag atggtgccga tgatgacatg caggccatgg aacccggtcg
    15421 ccatgaagaa ggtcgagccg taaatggcgt tgagattgcc catgccgccg gtcgccgatg
    15481 cggtcagcga tttatgcgcg acatccatga acggcatcat ctgggcgtgg ttgaagccga
    15541 aggtgaacgc ggcatgggtg tattcataag cctgtacgca ggtgaaactc gcgccaagcg
    15601 cgatggtgac gagaagcccc tggatcgcac ccttgcggtc gctcttgagg atcgcatgat
    15661 gtgcccaggt gacggtggcg cctgaggtca gcaggatcag cgtgttgagc agcggcaaat
    15721 gccaggggtc gaaaggcacg atcccttccg gcggccaatg cccgatgccg acatcgagcg
    15781 agaacagcgc cgaattgaag aacgcccaga accacgcgaa aaagaacatc acttcggagg
    15841 cgatgaacag gatcatgccg tagcgcaggc ccagcttgac caccggggtg tgatcgccct
    15901 tgacgaccga ttccttcacc acgtcgcgcc accagcccgc catggtgtag agcaccagca
    15961 cgaagccgat gatcatgatc cagggctgac cggcaatcgg aaggccaaag aaattggcgc
    16021 cggtgttcat ccagaacacc gcgccgagca gcatcacgaa cgcggaaaac gaaccgacga
    16081 tcggccacgg actggggtcg accagatgat aatcgtgatg cacgtcgccg tgagccatgg
    16141 tgttcgcctt tcgaaggatc agccccccga acccttcgcc ggaacggccg cggtcttttt
    16201 cgcatccgtc gtttgattgg tcgcgggata aaaagtgtag gacagagtga tagcggtgac
    16261 cgtatcttcg tcgtggtttt tcaggatcgt gggcgagacg aagaaaatta ccggcatctc
    16321 gacggattcg cccggctcga gccgctgctc ggtaaagcag aagcattgga tcttattgaa
    16381 atagcgcgcg gtgtcgtcag gcgtgacatt gaatgtggca tggccgacga tcggtttatc
    16441 cgagcggttc accgcatgga aatacaccag cttctgctcg ccgggcttca cgttgaccgc
    16501 gcgctgttcg ggaagaaaac tccaaggcag cgccggatcg atattggtgt cgaagcgcac
    16561 ggtgaattcg cgcgttccca cggcgcccgg cgcggcatcg gcgcgccgcg gcgtgccgcc
    16621 atagccggtg gcgcggcaga acgcgtaata gagcggcacg gcggcatagg acattgcgac
    16681 catgccgatg acgacgccaa agcagcagcc aaataccgcg agattgcgcg tgatcttcat
    16741 tgccgcacct cccgtctaat ccgccacatg tccacccagc cgcaccatgg tcatgatgaa
    16801 gaacagcacg acgagaaaga gcagcgtacc cgcaatggcg taattgcgca tgcggcgggc
    16861 gcgttcgaac cgctctttgg caaagcgtgc tcttgcctcg tcatcgtcgc cgttcatccc
    16921 acagccagag tgtgcaggtg cgaccatccc gcgacctgct cgatcagaag cgcggcgaac
    16981 agcgcgaaca gataggcgat cgaaacaccg aagagccgcc gcgcctgggg ctcgcgcaca
    17041 atgtcgcgtt cgcgcgcgac gcgcacactc tggatcagga accaaagccc caacccaaca
    17101 ttggcggcga gatagagagg cccggcaaag cccaggatcg ttggcgcaaa accgctcgca
    17161 acgacgagtg ccgtataaag cacaatctgc cggcgggttt cggccttgcc cgaaacgacg
    17221 ggcagcatcg gaacgcgtgc gcgcgcataa tcctcggcgc gataaagcga cagcgcccag
    17281 aaatgcggcg gcgtccacag gaagatgatc gccacgagca ccagcggcgg caccgccagc
    17341 gagccggtcg ccgcggccca gccgatggcc ggtggaagcg cgccggccag ccctccgatg
    17401 acgatgttct gcggcgtgcg gcgtttcagc cacatggtgt agaccaggac atagaagaga
    17461 atcgtgaagg caagcagagc cgcggcgacc agattgacga gcaggcccat gaccgtgacc
    17521 gcgccgatgg aaagcgtggc tccgaacgcg gccgcggtgc cggccgtcag atggccttgc
    17581 ggaacgggcc ggcccatggt gcgcgacatc agcgcatcga tgtccgaatc ataggccatg
    17641 ttgagcgcac ccgatgcccc tgcccccacc gcgatgcaca aaagtgccgt gatcgcggtc
    17701 gccatgtcga gatgtcccgg cgcgagcaca aggccgacaa tccccgtgaa cagcacgagc
    17761 gacatgacac gcggtttgag cagcgcgaaa taatcgctta cggacgcgaa attcaactcc
    17821 gcgtggacga tcgactccgg cctttcagca acgacgctca tgcttcaccg tctcagtgat
    17881 gaccgccatc ggtaattcgc ggcaggtcgt tgaaggtgtg gaagggcggc ggggaagaca
    17941 gcgtccactc cagcgtggtt gcgcccgcgc cccagggatt atcgcccgcg acgcgctttt
    18001 tcatgatcgc ttcggccatg cacagaagga acaccaggac gctggccgcc gaaatgaatg
    18061 agccgataga ggcgacatag ttccacccgg cgaaggcatc agggtaatcg gcgatgcggc
    18121 gcggcatgcc cgcaaggccg acgaaatgca tcgggaagaa ggtcaggttc acgccgacga
    18181 acatcaccca gaagtgcagc ttgccgagcg tctcattgta catgtagccg gtgatcttcg
    18241 ggaaccagta gtaccagccc gagaagatgg cgaagaccgc acccagcgac agcacgtaat
    18301 ggaaatgcgc cacgacgtaa taggtgtcct gcaacaccac atcgacaccg gcattggcca
    18361 gcaccacgcc ggtcacgccg ccgacggtga agaggaagat gaagccgact gcccagagca
    18421 tcggtgtgcg gaagctgatc gaaccgcccc acatcgtcgc gatccaggag aatatcttca
    18481 cgcccgtcgg caccgcgatg accatggtgg cgaagacgaa atacgccttg gtgtcgacgg
    18541 tcaggcccac cgtgtacatg tggtgcgccc agaccacgaa gccgatgaag ccgatcgcga
    18601 ccatggcata ggccatgccg agatagccga acaccggctt cttggcgaac gtggaaacca
    18661 cttggctgac catgccgaag cccggaagga tcagaatgta gacttccgga tggccgaaga
    18721 accagaacaa atgctgatac aggatcggat cgccgccgcc ggccggattg aagaatgtgg
    18781 tgccgaaatg ccggtcggtc agcagcatgg tgatcgcacc ggcaagaacg ggcagcgaaa
    18841 gcagcagaag gaacgtcgtc accaggatcg accacacgaa gagcggcatc ttatgcagcg
    18901 tcatgccggg cgcgcgcatg ttgaagatgg tggtgatgaa attgatggcg cccaggatcg
    18961 aggacgcgcc cgcgatatgc agcgcgaaga tcgcgaggtc catcgccggt cccggcgagc
    19021 cataggtgga aagcggcgcg tagatggtcc agccgccgcc cactccctca ccgccggaat
    19081 cgccttccac gaacatcgaa aggatgagca gcgtgaagga gaacggcagc agccagaacg
    19141 agatattgtt catgcgcggg aacgccatgt cgggcgcgcc gatcatcagc ggcgtgaacc
    19201 agttcccgaa gcccccgatc atcgccggca tgatcatgaa gaagatcatg atcagcccgt
    19261 gacccgtgac gaacacgttg aaggtgtgcg gatccttgaa gatctgcatc cccggataca
    19321 tcagctcggc ccgcatcatc accgagagaa atccgccgat cagcccggcg cagatggcga
    19381 acacgaggta catcgtgccg atgtccttgt ggttcgtcga gtaaacatat cggcgccaac
    19441 catgaggatg gctttcatgg cccgtatgga ccgccgcacc gtgtgtgtcg tgtgcacctg
    19501 ccatggcgct atcctcgctt cccttaatgg gccacagcga cgcgcgtggg cgcgccgact
    19561 gcggcgaatt tggtttttgc ctcggcgacc cacatgtcga atttttcctg gctcacgaca
    19621 tcgaccgtga tgggcatgta ggcgtgacgc gcgccgcaga gttcggaaca ctggccataa
    19681 taggtgcctt ccttgtccgc cttgaaccag ctctgattca gacggcccgg cacggcatcg
    19741 atcttcacgc cgaacgccgg aagagcccat gagtggatga catccgcccc tgtggtgatg
    19801 agacgaacgg tcttgccgac cggcaccacc acatgattgt tcgtcccgag catccgcggc
    19861 ggaccgttcg gaaccaccgg ctgcgaccgt ctgtcggcga cgatctgcgc atcgaaggtg
    19921 aaattgccgt tgtccggata ttcataggtc cagaaccatt gcttgccgat ggccttgacc
    19981 gtcatgtcgg ccggcgggat cacgtcttca aaatagagca ggcggaagga tgggatggcg
    20041 atcgccacca ggatcaacac cggcacgacc gtccacagca cttcgagcgt ggtgttgtgc
    20101 gtggtggccg acggcgtcgg attggccttc gcgttgaagc gcagcgcaat ccacaacaaa
    20161 agcgcaagta cgaacagcga caccgcggtg atgatgtaga ggacgaatgt gtgcagcccg
    20221 atgatgtcgc gcatacgttc atcggcggcc ggctgcaatc cgatctgcca aggcaccggc
    20281 tgggcggacg cggacacggc aaaggacagc atggccgtgg tgaataacgc cgtaaacgcg
    20341 ccgagttttc ggatcatgac gcccctcatt tggacttgca gccgaggatg gggctctccc
    20401 tcccctgtcc cggctcgcgc ggaagattct tagtgcacga tttaggcggg cggcagtggc
    20461 tgagtcattg cgaccagatg ccacgtaggg ctgttatccc cctcatcccg cttgagcttt
    20521 cgtcccatat tctcgaccca ggtgacgggc gggcagcgca tgcctatata gtttggaccg
    20581 gcaatctttc cggcaatccg aacccgagca agcccatgac cagccacgaa gacctgtttt
    20641 tcacccaaac cgggatggag aggcgccgcg tccagggtct catcgacaat acgctgcaca
    20701 gcgccgatga cggcgagctg ttcctggaat accgccagac cgagagtttt tccttcgacg
    20761 acgggcgtct gaaggctgcg acctacgata cgacccaggg ttttggcctg cgggccgtcg
    20821 cgggagaagc caccggttat gcccacgctt cggagctgtc ggaagcagcc atcgcgcgcg
    20881 cggccgaggc ggtcggcgtg gtcaagctcg gccattccgg cacgctggcg ggcggtccgg
    20941 cacggaccaa tcttcacctc tacaccgaca acagcccgct cgattcggcc agtttcgaca
    21001 tcaaggtcaa actcatggcg gagatcgacg cttatgcgcg ttccgccgac ccccgcgtca
    21061 agcaggtctc ctgttcgctg gcgggcgaat ggcaggtggt cgagatcatg cggccgggcg
    21121 gcgaatccta tcgcgacgtg cggcccctgg tacggctgtc gatttcggtg gtcgtcgagg
    21181 agaacggtcg gcaggagacc ggctattacg gcggcggcgg acggcgtgct tacaccgact
    21241 acatcgcgcc cggctattgg cgcgccggcg tggatgaggc attgcgcagc gcgctggtga
    21301 atttgcgctc cgtccccgcg ccggccggcg agatgagcgt cgtgctcgga ccaggctggc
    21361 cgggcatcct gctccatgag gcagtcggcc atgggcttga gggcgacttc aaccgcaaga
    21421 agacatcagc cttcgccggc ctgttgggcc agcgggtcgc ctcccccggt gtcacggtgg
    21481 tcgatgacgg caccatctcg gagcgccgcg gctcgctcac catcgacgat gagggcaccc
    21541 cgacctcgcg caccgttctg atcgaggacg ggatcctgaa gggtttcatg caggaccgca
    21601 tgaatgcgcg gctgatgggt gtcgctccca ccggcaacgg ccggcgcgag tcctttggca
    21661 gccaggtgat gccgcgcatg acgaacacct acatgctgtc cggccatcaa gcgccgcagg
    21721 agatcctcgc ctccgtcaag aatggcatct atgcggtgca gttcggcggc ggccaggtcg
    21781 acatcaccag cggcaaattc gtgttctcct gcaccgaggc ctatcgcatc gaggacggca
    21841 aaatcggtgc ccccatcaag ggcgccacct tgatcggcaa tgggccggac gcgatgacgc
    21901 gggtttcgat gatcggcaac gacatgaagc tcgacaccgg catcggcacc tgcggcaaga
    21961 acggtcagag cgtgccggtg ggcgtgggtc agccgacgct tcgcatcgac gggctgacgg
    22021 tcggcggtac agggggctga cgcctcagcg cgcttcgggc gcgctggcgc gtttgacctc
    22081 gggataatat tgcggggctt cgcggcggtc gaacatgccg taatcgcgca ccacccgcac
    22141 attgcgaagc ctggcgccgt ccggcaatga gacgccgcgc tcgaagcgtt ccgccgcact
    22201 cacatcgcgc caggacgaca tcaggatgat gtcccctggc gtcaaaatcg cctcgaaaac
    22261 atcccaggcg acaagaccgg gcgcctgcgt cgcgagaccg agccatgtgg cggcatcctc
    22321 agatctggct tgcttgaccc attcgggcgg tctcttgccg tcgatcaagg tcacaatggt
    22381 gccttcgcca atttcggttt cgtccagcct ctgttctcgc aacacacagc ctgccggagg
    22441 cctggtatcc cgtgtgatct cgccgaccct gagatgatag tccgcgaaga tttcggaccg
    22501 ccctttctcc tgtgcctgac ggtggcgcgc aacggtgcgc cagcgaacca ccgatttctc
    22561 gtcgcgccag cttgaaagcg acagcagcca tccctcgcgc gtgaggctgc gataccggat
    22621 gttgtcggcg aagccctcga tttgctcaag ctcgggtttg agcatcctgg ccgtgtcgag
    22681 ataggcgtcc caacggtcgg ctttcggatg aacttcaaac agtacggaaa acatcgggcg
    22741 ccctattggc gcgcttcagc gtgcgcattc tgggagttgc gtttgggcag tacccagttg
    22801 ggacggacga aatgacaggt gtaccctgtg gggtagcgtt ccagataatc ctgatgctcg
    22861 cgttcggctt cccagaaatc gctcgccggc gtgacctcgg tcaccacctt gcccggccat
    22921 aggcccgaag cttccacatc ggcgatggtg tcgagcgcgg tctctttctg cccttcgctg
    22981 gtatagaaaa tcgccgagcg atagcttgct ccccggtcgt tgccttgccg gttcagggtc
    23041 gtgggatcgt gaatctgaaa gaagaattcc aggagcttgc gatagctgat cttctggggg
    23101 tcgaagacga tctcgatcgc ctcggcatga ttgccatgat tgcggtaggt ggcgttggcc
    23161 acatcaccgc ccgaatagcc gacccgggtc gaaacaaccc cgggataacg gcgcaataaa
    23221 tcctgcatcc cccaaaagca gccgccggcg aggactgcgc gttccgtgga agctgccatg
    23281 ttcattcctc ttatggggtt gtgcacatcc acgcggcatt ctccacgctc aggccgaacc
    23341 ggaccagcgc aaatccaccg cagctttcca ccatgtatgc atcgagatgc ggttcttgaa
    23401 ggccttcgcg atgcatcttt ttgccctcgc atagacgcga ctttttaccg gcggtcattt
    23461 gcgcgttgca aatgaaatgt tgcagcgcaa tattagccaa cgcggcctta acctgccgag
    23521 gatcatggct gaacggcacc ttgcaattgt ttcatcgctg gctcccggca ccgccagccg
    23581 cgaatcggta cgccttctgg ggcgccaatt gggcgagatc atccgggagc agcacggtca
    23641 ggccgccttc gaccgcgtgg aaaatttgcg ccgtcacgtc gtgcatgaac atcgcgaagg
    23701 gcgttccgcc gttgcgttgt tgcggcaatt cagtcatctg ccgaaccgcg atctcaccat
    23761 cctgatccgc agcttcgcga tcttctcgca gctcgccaat atcgccgacg attatatcgc
    23821 acgctgcgaa gtggaaagcg acgattgcga ggactcgctg gaagcattgc ggtcccaccc
    23881 caaaatgacg gcggatcgag tctatgccta tctcaacggc gcgacggtcg ctcccgtcat
    23941 caccgcgcac cccaccgagg tccggcgcaa aagcgtgatc gaccgggaat cggcgatcgc
    24001 cgatctgctg cccgcctatc atcatttcgc gccaggcaag caccggcgcg ccgagatcga
    24061 gactcagatc aagcgggaaa tccgcatgct gtggcagacc cgcatgctgc gccccgaacg
    24121 catccgggtg accgacgaga tcgactacgc ggcctcgatc ctggggcaca ccttcgcctc
    24181 gcaattgccg gtggtgaagc gcaagctcgc gcatgtcttc ggattgcagg gccccctgcc
    24241 ccgctcgctt cgcgtcgggt cctgggtcgg gggcgaccgt gacggcaatc ctttcgtgac
    24301 cgcgcagacg ctggattatt cggtgcggca tctggccggg atcgtgttcg atcattacct
    24361 ggagcaattg catcttctgg gcgcggaact gtcgctttcc gacgagcttg cgccggtcag
    24421 caaagcgctc accgcccttg cggcgaacgc gcctcacgca tcgttgcaca aagccgacga
    24481 gccttaccgg caggcattga cctacagcta tgcacggttg tcgagcaccc ggaagattct
    24541 gctcggacac ggcgccatgc gtccgccgcg cacggaggcg gagccctatc ggaacgtgta
    24601 cgaattgtcg gacgaactcg cggtcgtcgc gaactcgctg atccacaatg gcagcgccga
    24661 tctcgccgac ggacgcctgg ccgaattgcg cgaggccgtg gaaagtttcg gctttcattt
    24721 cgcggtgatg gatttgcgcc agaactcggc ggtccacgaa cgcgtcgtcg gcgaactgct
    24781 gcgcgaggcc ggggtgagcc aggactatgc ggagctggcc gaagacgcac gcgtggccct
    24841 cctgctcaag gaactcgaca atccacggct gttgcgcacg ctctatcgta cctattcgga
    24901 ggagacggcc aaggaactcg acatcgtcga tatgacggcc cgtctgcgcc ggcaattcgg
    24961 cgagagcgcg gtcgagaact atgtcatctc gatggcgaaa agcgtctcgg acatcctcga
    25021 agtcgccatc ctgctgaagg aagccggcct cttcataccc ggagccaagc cacaggcatc
    25081 gctgcgcatc gttcccctgt tcgagacgat cgaggatttg cgcgacagcg cgcgcatcat
    25141 ggaagcttat ttcaccctgc cgctggcgcg ttcgatcctt gccgcgcagc acgatctgca
    25201 agaggtgatg atcggctatt ccgacagcaa caaggacggc ggctacgtca cgtcgaactg
    25261 ggaaatccgc gccgccatcg tcaggctgat cgcgctcgcc aaatcgcggg gcttgcgtct
    25321 gcgtttcttc catgggcgcg gcggctcggt cggacgcggt ggcggcccga gcttcgaggc
    25381 gacgcgggcg tttccggcgg gcgcggtgga gggcggcatt cgcgtcaccg aacaaggcga
    25441 ggtcgtggcc agcaaatacg gccatcccga tgtcggccga cagactctgg agcgcatggt
    25501 ggcggccacg cttctggccg acatcgatcc cgagaccgat gccgccgatg gagcgctggc
    25561 cgaaagcttc gcaatcttca gcgcggaggc ctatcgcgcc tatcgcgcgc tggtctacga
    25621 gacgccgcgc ttcgatctct atttccgcca atcgacgccg ctgccggaga tttccgatct
    25681 gaagatcggc agccggcccg catcgcgcaa gaattccacc cgaatcgaag atttgcgcgc
    25741 cattccctgg gtcttcagct ggtcgcaggc acgcctcaca ttgccgggct ggtacggctt
    25801 cggagcggcg gcggcaaaga tgcaggagat ggggcgcgac gcggaattga ccgcgctcta
    25861 tgcccagtcg cgcttcttcc gcaccgtggt gtcgaatctg gagatggtgt tggccaaatc
    25921 caatctggat atcgcccggc actatgccga tctcgttgaa gatcagacac tggcaaatgc
    25981 catcttcgcg cgtatcgagg ccgaacatcg cgccacgctg gacaccgtgc tggctttgac
    26041 agggcagaag acgctgctcg aaaataaccc cacactggcc gaatccatca ggctcaggct
    26101 gccctatatc gatgcgctca atctcttgca gctcgacctc ttgcgaaggc gccgttccgg
    26161 caaggaagac cccgaaacgg tgcatgccat tcacatgtcg atcaacggcg tgtccgccgg
    26221 tttgcgcaac agcgggtgat tgacccgaat aagtcgagcg catagcctat tggcgcgatg
    26281 aaccaatatt ggtttcgacc caaacagcgc ggcatcggcg ccggcgtgcc gctcaattgg
    26341 aagggctggg cgctgttcgc gacctatgtg gtggcgatcc tggccgtgcc gtccttgtac
    26401 gaattatatc tcggctatcc cggcacgccg ttgctgcgtg ttcttggaat cgtcgcggtc
    26461 tctgttccgt tcctgatcct cgcctggaag aagaccgaag gcggatggcg ttggcgccgc
    26521 ggcgccgaag atgccgatga cggcaatccg gcctgaggtg cctaagcgtc ctataggaaa
    26581 ttataggcct cgaacaattt gcccagatcg cccttccatt cgccgcgata gagcgccagc
    26641 aattcatcgg cccgcgtctg gccgcgctcg accaattcct gcaaaggctg aagaaagccc
    26701 tcctccgtcc cgccgctgga atcgagcgcg ccacgcgctt gcaggccatg cgccgcaagt
    26761 tccagcatgc ggcgagccag ttccagcaga ttggtttttc ggaacggcgt cttgaacgca
    26821 agcttcggca ccgtggcgcg catgctttcg cgctcctccg ccgtccaatc tttcaccaga
    26881 tcccaagcca catcgagcgc cgtcgaatcg tagaagatcc cggcccacaa ggccggcaga
    26941 ccgcagatgc gccgccacgt gccgccgtcc gcgccccgca cttcgagata ggatttcaac
    27001 cgcgcttccg ggaagatcgt cgtgaggtga tcggcccaat ccgacatcat gggcgtgatg
    27061 cccttcaatt cggcaatccg gcccgtcaga aaatcgcgaa acgactttcc cgccacatcg
    27121 atgtagcggc cgtcgcgata gacgaaatac atcggcacat cgagcgcgta atcgacatag
    27181 cgctcaaacc cgaaaccgtc ctcgaacaca aagggcaaca ttccggcgcg gtcattgtcg
    27241 acatcggtcc aaatctggct gcgcagcgac aggaacccat tcgccttgcc ctcgcggaac
    27301 ggcgaattag cgaacagcgc ggtcgcgacc ggctgcaacg caagcgccac gcgaaacttc
    27361 ttcaccatgt cggcttcgga gccgaaatcg aaattcccct gcaccgtgca ggtgcggaac
    27421 atcatctcca gcccatagcc gccgaccttg ggcatatagc gccgcatgat ggcgtagcgt
    27481 cctttgggca tcaccggcac gtcgtccagc ctccaggtgg gcgcatagcc gacgccgaga
    27541 cagcccgcct cgatgtccgc gcagacctcg cggacctgct tcagatgcgt gttcacctcg
    27601 acgcaggtct catgcaccgt cttgagcggg gcccccgaaa gctcgaattg cccgcccggc
    27661 tccaggctca acgcggcccc gttttgcgac aggccgatga tgttctcgcc ttcca
//
LOCUS       DASYEG010000138         9334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4247906, whole genome shotgun sequence.
ACCESSION   DASYEG010000138 DASYEG010000000
VERSION     DASYEG010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 9334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9334
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4247906"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VGM72_06165"
     CDS             complement(<1..249)
                     /locus_tag="VGM72_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY4264885.1"
                     /translation="MASKGPRKANGGFDLSQSPSHLLRRAVQYANDLFSREPGASDLT
                     KQQFTVLAAVEQNEGMSQTDLVAITGIDRSTLAEMIRRM"
     gene            complement(490..1053)
                     /locus_tag="VGM72_06170"
     CDS             complement(490..1053)
                     /locus_tag="VGM72_06170"
                     /inference="COORDINATES: protein motif:HMM:NF001323.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEY4264886.1"
                     /translation="MHPLLKLALDLGPLLVFFAANAMFGIFPATAIFMVTMLVVIVIG
                     IAVERKLSPMPLVTGALVLIFGGLTLWLSNDIFIKIKPTILYVMFAGTLLGGLVFGRL
                     FIKLMLGQTLNLPEEAWRALTWRWAFFFIALAILNEIVWRNVSTNAWVAFKLWGVFPL
                     TLLFAMAQTPFIARHQIESDETSAASR"
     gene            complement(1106..2086)
                     /gene="ftsY"
                     /locus_tag="VGM72_06175"
     CDS             complement(1106..2086)
                     /gene="ftsY"
                     /locus_tag="VGM72_06175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEY4264887.1"
                     /translation="MTNEEGSGARLGMFARLKTGLARTATQLSGGLTGVFTKTRLDEE
                     TVRALEDALIRADVGTGLARELAAEVARDRYDTDITELELRRLLADAIAKVLAESAKP
                     FAIDPARKPFVVLVAGVNGTGKTTTIGKLASKLGEQHKRVWLAAGDTFRAAAIDQLKI
                     WGERAHAPVIARTPGADAAGLAFDAFSEASAGNADVLLIDTAGRMQNKTGLMSELQKI
                     ARVLKKQDADAPHAAILVLDATTGQNAISQVEAFAAAVPLTGLIMTKLDGTAKGGILV
                     ALAHRFKLPIHFIGVGESIGDLQSFDALSFARALTGADEPPRPTMGDFRF"
     gene            complement(2083..3366)
                     /gene="mtaB"
                     /locus_tag="VGM72_06180"
     CDS             complement(2083..3366)
                     /gene="mtaB"
                     /locus_tag="VGM72_06180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEY4264888.1"
                     /translation="MSSVEILTFGCRLNAYESEAIRARAKDAGLEDAIIVNTCAVTAE
                     AVRQSRQAIRKARRENPGTEIIVTGCAAQIEPESYAGMPEVDRVLGNREKLEARSYAR
                     DFLGDAPRVLVNDIMAAKETAAQFVDHFPEHARAILQVQNGCDHRCTFCIIPYGRGNS
                     RSVGAGAVVEEARRLVAAGYNEIVLCGVDLTAYGADLPGKPTLGMLAARILKHVPELA
                     RLRLSSIDSIEADETLLRLIGDDERLMPHFHLSVQSGDNLILKRMKRRHTREDTIAFC
                     ETVRRLRPDVAFGADLIAGFPTETDAMFQNTLALVDEAGLSYLHVFPFSAREGTPAAR
                     MPQLAKQTIKERARLLRQKGDAAFSSRLAKLVGTEQELLVEKPGVGRTRCFASAMFED
                     DAAPGRLIRTRIVSTAGRIAQARVMNAVQQSPPRP"
     gene            complement(3363..4214)
                     /gene="dapF"
                     /locus_tag="VGM72_06185"
     CDS             complement(3363..4214)
                     /gene="dapF"
                     /locus_tag="VGM72_06185"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEY4264889.1"
                     /translation="MTSFLKMHGLGNDFVIFDARTFPVPIDAAAARAIADRKRGIGCD
                     QVIVMEPAKNGAAAFMRIFNTDGGEVESCGNAARCVAYLLMAERETDHIGIATAGGPL
                     ACSGTGADAVTVDMGVPRLDWREIPMAQAVDTQSFALDVPGFADAALQSAAAVSVGNP
                     HCVLFVEDAERAPVAALGPAIERHPWFPSRTNVEFVERRGDSALRMRVWERGAGITLA
                     CGTGACAAAVASFLRGLTGRKVKLILDGGELEIEWRESDDHVLMTGPTRFSFAGEVNI
                     PDLVLPA"
     gene            4305..6425
                     /locus_tag="VGM72_06190"
     CDS             4305..6425
                     /locus_tag="VGM72_06190"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY4264890.1"
                     /translation="MWRYPLRARIGMGLLAGLALIFGMVTAMYGVDAGDDAYLWLEDI
                     QGAKPLEWVNEQNAVTLKRLKSDPDYQKDYDAILSVLDADDRIPIGELHGESVFNFWQ
                     DSTHVRGIWRRTSIASYETPNPQWEVLLDIDTLAAEEGKNWVFKSASCSPDLSRCLIS
                     LSPGGGDTTVLREFDPAAKRFVDEGFALGEAKAEAVYMDANTILFGTDFGTVTLTHSG
                     YPRIVKMWRRGQRLEDSKIVFEGKPADVIVSPGVIHSRNGSIAVVSRSVSYFDTEYSY
                     VTSDGGTIPLPLPLSAHLRAAVGDDLFLILRKDWKPDDQALIKQGSLIAFSLKEFLAT
                     RKLPKISILYAPGPRATIDAVVSGRDALYVAILENVIGSVHVFRPNGSAGWKETRLDL
                     PAGGSAGISSANDFGPEAYFAYQGFLEPTTLFVAKNDGRPVAFKSLPARFDATPYASV
                     QYEATSKDGTKIPYFVVRAKDATGPTPMLLYGYGGFEISQTPFYWTSMGRVWLPKGGA
                     YAVANIRGGGEFGPGWHEAAVKTNRQKSFDDFIAVAEDMEKRGLTTPRQLGIMGGSNG
                     GLLVGTVAVERPELFGAVVCQVPLLDMLRFSKLGAGASWVGEYGDPDNPFERSAILRY
                     SPYQNVKSGVAYPPFLFVTATSDDRVTPVHARKMAAKMEAQGHDVLFYENTEGGHAAA
                     ANHAQAAEMNALGYVYLAQKLGLN"
     gene            complement(6418..6825)
                     /locus_tag="VGM72_06195"
     CDS             complement(6418..6825)
                     /locus_tag="VGM72_06195"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY4264891.1"
                     /translation="MASAHGSCLCKTVQYEVDLPFERFMYCHCSRCRKATGSPHAANG
                     FVQASAFRWMQGEAQVKRYDVPEAKRFGTQFCSNCGSKVPHHTRDHAWMVIPAGSLDE
                     DPGMKPQRVVFWGSRAPWFTDCSEMTKHDEMPS"
     gene            6989..8410
                     /gene="ffh"
                     /locus_tag="VGM72_06200"
     CDS             6989..8410
                     /gene="ffh"
                     /locus_tag="VGM72_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY4264892.1"
                     /translation="MFDSLQDRLGGVFDRLRGRGALSEADVETALEEVRSVLIDADVA
                     LPVVKTFIGKVRERAVGEQVIRSVTPGQQVVKIVHDTLVETLGVESEGLSLGAPPASI
                     LMVGLQGSGKTTTSAKLANLLQTKDKKRVLMASLDVNRPAAMEQLRVLGEQANVPTLP
                     VVLGQRPTDIARRALAAARVGGYDVLILDTAGRQHVDEALMAEVAEVKKLTNPHETLL
                     VADSLTGQDAVNIAKSFNERVQISGIILTRTDGDARGGAALSMREVTGAPIKFLGTGE
                     KLDALEVFHPARVASRILDMGDVVSLVEKAVEHVDREKAEKLAKKMKKGEFDFNDLAD
                     QLSQMRKMGGMQGLLGMLPGVGKIKDQLAAAGMDDRVLKRQEAIISSMTREERGKPNV
                     INGSRRKRIAAGAGVEVSDVNKILKMHRQMSDVMKKMGKGGRGMLGALGGMNPNMLAG
                     LGGGGGAGLPPGFSGLPPGMTRK"
     gene            8438..8677
                     /gene="rpsP"
                     /locus_tag="VGM72_06205"
                     /pseudo
     CDS             8438..8677
                     /gene="rpsP"
                     /locus_tag="VGM72_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003092643.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
     gene            8791..9297
                     /gene="rimM"
                     /locus_tag="VGM72_06210"
     CDS             8791..9297
                     /gene="rimM"
                     /locus_tag="VGM72_06210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEY4264893.1"
                     /translation="MTQSRVLLGVVAAAHGIKGEVKVKTFTESPQALGAYGPVTTEQG
                     RTLEIVQLRPTKRDEAVVRFAGLSDRNAAEELRGAQLSVPRAALPPPEDGAFYHADLV
                     GLRAEDRSGSVLGKVLGVHNFGAGDVLEIEFTAGGTEFVAFADPHVPVVDVAGGRIVI
                     ELPRENQN"
BASE COUNT         1786 a         2840 c         2939 g         1769 t
ORIGIN      
        1 catccggcgg atcatctccg caagtgtcga acgatcgatg cccgtaatgg cgacgaggtc
       61 tgtctggctc atgccttcgt tctgctcgac cgcggcaaga accgtgaatt gctgcttggt
      121 cagatcgctg gcgcccggct cgcgcgaaaa cagatcattc gcatattgca cggcgcggcg
      181 cagaagatgc gacggtgact gagaaaggtc aaacccgcca ttggccttgc gcggaccctt
      241 cgaagccatc cccaaccccc ggtgcaatgt cgagatttca gttcaaattc gtccgtaccc
      301 gtcggtacgc agttcaacct tagcgcaacg catcgcggac gcaagcagtc tttgagcgat
      361 tttgatcgaa tgatgcatga gagtttcact tgctgtatcg atgcgggtgt tgcgttgtcg
      421 caacgcgaaa ggcgcgtggg cgcgtatcct ccagcggggc cggtttattt cgaaaagctc
      481 ttcaaccgtt taccgcgacg cggcggacgt ctcatcgctt tcgatctgat gacgcgcaat
      541 gaacggcgtc tgcgccatcg cgaaaagcag cgtcaacgga aatacccccc acagcttgaa
      601 cgcgacccat gcattggtcg agacatttcg ccatacgatc tcattgagga ttgcgagagc
      661 aatgaagaaa aacgcccagc gccatgtgag cgcgcgccat gcctcctcag gaagattcag
      721 cgtctgtccc agcatcaatt taatgaacag ccgtccgaat acgagcccgc ctaataatgt
      781 gcccgcgaac atgacataga gaatggtcgg cttgatcttg atgaaaatgt cgttggacaa
      841 ccatagggtt aacccgccga aaatcagcac cagcgcgccc gtcacgagcg gcatcggcga
      901 aagtttgcgc tccaccgcaa tgccgatgac gatgacgacc agcatcgtca ccatgaatat
      961 cgccgttgcc gggaaaatgc cgaacatcgc gtttgcggca aaaaaaacca gcagcggccc
     1021 gagatcgagc gccaatttca gcagcggatg catgaagaca ccataggggt ttgcgtacaa
     1081 ttgttccagc ccgtgaaacg agcattcaaa accgaaaatc gcccatcgtc ggccgcggcg
     1141 gttcgtcggc tccggtgagt gcccgggcaa aggacaaagc gtcaaagctc tgcagatcgc
     1201 cgatgctctc gcccacgcca atgaaatgaa tcgggagttt gaaacgatgg gcaagcgcga
     1261 ccaggatgcc gccttttgcc gtgccgtcga gcttggtcat gatgaggccg gtcagcggta
     1321 ccgctgcggc gaacgcctcc acttgcgaga tcgcgttttg tcccgtcgtc gcatccagca
     1381 ccaggatcgc ggcatgaggc gcgtctgcgt cctgtttttt caggacacgg gcgatttttt
     1441 gcaactcgga catcaacccc gttttgttct gcatgcgtcc cgccgtgtcg atgagaagca
     1501 catcggcgtt gccggcgctg gcttcgctga aggcgtcgaa tgcgaggcct gcggcatcgg
     1561 cgcccggcgt acgcgcgatc acgggtgcat gtgcgcgttc cccccatatc ttgagttgat
     1621 cgatggctgc ggcgcggaat gtatccccag cggccagcca gacgcgcttg tgttgctcac
     1681 caagctttga cgcgagcttt ccgatggtgg tcgtcttgcc ggtcccgttc acgccggcga
     1741 ccagcaccac gaaaggcttg cgcgcgggat cgatggcgaa gggtttcgcg gattccgcca
     1801 gaactttggc gatcgcatcg gcgagcaggc ggcgtagctc caattctgta atgtcggtgt
     1861 cgtaacgatc tctggcgact tccgccgcga gttcgcgggc aagcccggtg ccgacatcgg
     1921 cgcggatcag cgcgtcttca agggcccgca cggtctcctc gtccaggcgc gttttcgtga
     1981 aaacgccggt gaggccgccg gaaagctgcg tcgccgtgcg tgcgagaccc gttttcaggc
     2041 gcgcgaacat cccgagcctg gcgccggaac cttcctcatt cgtcatggac gcggcggact
     2101 ctgctgcacc gcgttcataa cgcgcgcttg ggcaatgcgg ccagccgtgg aaacgatgcg
     2161 cgtgcggatc aagcggcccg gtgccgcatc gtcttcgaac atggccgaag cgaaacagcg
     2221 cgtacggcca accccaggtt tctccaccag cagttcctgt tcggttccca caagtttcgc
     2281 aaggcgcgag gaaaacgcgg catcgccttt ttgccgcaac agccgggcgc gttccttgat
     2341 cgtctgcttc gcgagctgcg gcatgcgcgc cgcaggtgtt ccttcccgtg cgctgaacgg
     2401 gaagacatgc agataggaca atcctgcctc atccaccagg gcgagcgtgt tctggaacat
     2461 cgcgtcggtt tcggtaggaa agcccgcgat cagatcggca ccgaaagcga catcggggcg
     2521 caggcgcctt acggtctcgc agaacgcgat cgtgtcctcg cgcgtgtggc ggcgtttcat
     2581 gcgcttgagg ataagattgt cgcccgattg caccgacaaa tggaaatgcg gcatcagccg
     2641 ctcgtcatcg ccgatcagcc gcagcagcgt ttcgtcggcc tcgatggaat cgatggaaga
     2701 aagcctcagc cgcgcaagct ccggcacatg cttgagaatt cgtgccgcca gcattccgag
     2761 cgtgggcttt cccggcaaat ccgcgccata ggcggtgaga tcgacaccgc aaagcacgat
     2821 ctcgttatag cccgccgcga ccaacctgcg cgcttcctcg acgaccgcgc ccgcgccgac
     2881 gctgcgcgaa tttccccggc cgtaaggaat gatgcagaac gtgcagcggt gatcgcagcc
     2941 attttgcacc tgcaatatgg cgcgcgcgtg ttccggaaaa tgatccacga actgcgcggc
     3001 cgtctctttt gcggccatga tgtcgttgac gagcacacgc ggcgcatcgc cgagaaaatc
     3061 gcgcgcgtag cttctggcct caagcttttc ccgattgccg agcacgcggt ccacttcggg
     3121 catgccggca tagctctcgg gttcgatctg cgcggcgcag ccggtgacga tgatctctgt
     3181 gccgggattt tcgcgccgtg ctttgcgaat ggcctgacgc gactgtcgca ccgcctcggc
     3241 cgtgaccgcg caggtgttga cgataatggc atcctcaagc ccggcatcct tggcgcgggc
     3301 gcgaatggct tccgattcat aggcattcaa gcggcaaccg aacgtgagga tctcgacgct
     3361 gctcatgcgg gcagcaccag atcgggaatg ttgacctcgc ccgcgaagct gaaacgcgtg
     3421 ggtccggtca tcagcacatg atcatcgctc tcgcgccatt cgatttcgag ttcaccgccg
     3481 tcgaggatga gcttgacctt gcgccccgtg agcccgcgta agaaggacgc aaccgctgcc
     3541 gcgcacgctc ccgtgccgca agcgagcgtg atgcccgcgc cgcgttccca gacacgcatg
     3601 cgcaaggcgc tatctccgcg ccgctccacg aattccacat tggtgcgcga cggaaaccac
     3661 gggtggcgct cgatagccgg gcccaatgcg gccacgggcg cacgctccgc gtcctccacg
     3721 aataatacgc aatggggatt gcccaccgac acggcggcag ccgattgcag cgcagcatcg
     3781 gcaaatcccg ggacgtcaag cgcgaaggat tgcgtgtcca ccgcctgcgc catggggatt
     3841 tcacgccaat cgagccgggg cacgcccata tcgacggtca cggcgtcggc gccggtgccg
     3901 ctgcatgcga gcggcccgcc cgcggttgca ataccgatgt ggtcggtctc gcgctccgcc
     3961 atcagaagat aggcgacgca gcgcgcggca ttgccgcagg attccacctc gccgccgtcg
     4021 gtattgaaga tgcgcatgaa agcggcggcg ccattcttgg ccggctccat cacgatcacc
     4081 tgatcgcagc ctattccgcg tttgcggtcc gcgatggcgc gcgcggctgc ggcatcgatc
     4141 gggaccggaa aggtgcgcgc gtcgaagatg acgaaatcgt tgccgagccc gtgcattttc
     4201 aggaaactgg tcatggccgc cttatagggc tgcggaaaac gacttgctag ctcccggctt
     4261 cgggatttga cgttaacgcc aaagcctaca aaatgagcag ccggatgtgg agatatccat
     4321 tgcgcgcgcg catcggaatg ggcctgctcg ctgggttggc gctgattttt ggaatggtga
     4381 cggcgatgta cggggtggac gcaggcgatg acgcctatct ttggctcgag gatatccaag
     4441 gggcaaagcc gctcgaatgg gtgaatgagc agaatgcggt cacgctgaag cgcctgaaat
     4501 ccgatccgga ctatcagaag gattacgacg ccatcctttc ggttctcgac gcggacgacc
     4561 gcatccccat cggcgagctt catggggaga gtgttttcaa tttctggcag gattccaccc
     4621 atgtgcgcgg aatctggcgc cgcaccagca ttgcttccta tgaaacgccc aatcctcaat
     4681 gggaagtgct gctcgatatc gacacattgg ccgccgagga aggcaagaac tgggtcttca
     4741 agagtgcgag ctgttcgccc gatctgtcgc gctgtctgat ctcgctttcg ccgggcggag
     4801 gcgacaccac cgtgttgcgc gagttcgatc cagccgccaa gcgtttcgtg gatgaaggct
     4861 tcgcgcttgg cgaggccaag gccgaagcgg tctatatgga tgcgaacacc attctcttcg
     4921 gcacggattt cgggaccgtt acgctgacgc attcgggcta tccgcgcatc gtgaagatgt
     4981 ggcggcgcgg tcagaggctc gaggattcga aaatcgtctt cgaaggcaag cccgccgacg
     5041 tcatcgtgtc gccgggggtg attcacagcc ggaacggctc gattgccgtg gtgtcacgct
     5101 cggtgagcta tttcgacacg gaatattcct atgtgacctc cgacggtgga acgatcccat
     5161 tgccattgcc tctgtcggcc catttgcggg cggcggtcgg cgatgatctg tttttgatcc
     5221 tgcgcaagga ttggaaaccg gacgatcaag cgctcatcaa gcagggctcg ctgatcgcct
     5281 tttcgttaaa ggaattcctc gcgacccgga aactcccgaa aatctcgata ctttatgcgc
     5341 cgggcccgcg ggccacgatc gatgcggtgg tctccggacg cgacgcgctc tacgtcgcga
     5401 ttctggaaaa tgtcattggc agcgtgcatg ttttccgccc gaacggcagt gctggctgga
     5461 aagagacccg gctcgacctc cctgccgggg gctcggccgg aatatcctcc gcgaatgatt
     5521 tcgggccgga agcctatttc gcctatcagg ggttcctgga acccaccacg ttgttcgtgg
     5581 ccaagaacga cggccgtccg gtggcgttca agtccctgcc cgcgcgcttc gatgcaacgc
     5641 cttacgcgtc cgtgcaatac gaggcgacct cgaaggacgg caccaagatc ccctatttcg
     5701 tcgtgcgggc gaaagacgcg acaggcccga cgccgatgct tctgtatggt tatggcgggt
     5761 tcgagatttc gcagacgccg ttctactgga cgagcatggg ccgggtctgg ctaccgaagg
     5821 gcggcgccta tgcggtggcc aatatccggg gcggcgggga gttcggtcca ggctggcatg
     5881 aggccgcggt gaagaccaac cgccagaaat ctttcgacga tttcatcgcg gtggctgaag
     5941 acatggaaaa acgcggcctg acgacgccca ggcagctcgg catcatgggc ggatcgaacg
     6001 gcggacttct ggtgggcacc gtggccgtgg aacgccccga gcttttcggc gcggtcgtct
     6061 gtcaggtgcc gctgctcgac atgctgcgct tttccaaact cggtgcgggc gcatcatggg
     6121 tgggcgaata tggcgatccc gacaatccct tcgaacgctc ggcgatcctg cgctattcgc
     6181 cgtatcagaa tgtgaaatcc ggtgtggcct atccgccgtt tctctttgtc accgcgacat
     6241 cggatgaccg tgtgacgccg gtgcatgcgc gcaagatggc cgcgaagatg gaagcccaag
     6301 gccatgacgt gctgttctac gagaataccg aaggcggcca cgccgccgcc gccaatcatg
     6361 cccaagccgc cgaaatgaac gcgctgggtt atgtgtatct ggcccagaaa ttgggcctca
     6421 actaggcatt tcgtcgtgct tggtcatttc ggagcaatcg gtgaaccagg gcgcgcgcga
     6481 gccccagaac accacccgct gcggcttcat gccggggtcc tcatcgagcg aacccgccgg
     6541 gatgaccatc caggcgtggt cgcgcgtatg gtgcggcacc ttcgacccgc aattggagca
     6601 gaattgggtg ccgaaccgtt tggcttccgg gacatcgtag cgtttcacct gggcttcgcc
     6661 ctgcatccag cgaaacgcgc tcgcctgcac gaaaccgttg gcggcatgcg gtgaaccggt
     6721 ggctttgcgg cagcgtgagc aatggcaata catgaagcgt tcgaagggca gatcgacttc
     6781 atattgaacg gttttgcaaa gacagcttcc gtgcgcgctt gccattagga ccctccttca
     6841 ttcgcctcga ttgacttaat ctataggcgg aactacaccc tcgccatccg gaatggctca
     6901 ggctttttcc ggtcctgggc gcaggctcgg gatcgccccc aggcagcgca ggtgcgcccc
     6961 ctggggcgtt cggcgtttgg agtatcgcat gttcgacagt ttgcaggatc ggctgggtgg
     7021 ggtgttcgac cgcttgcggg ggcgcggcgc gctctccgaa gccgatgtcg aaaccgcgct
     7081 cgaagaagtg cgcagcgtgc tcatcgatgc ggatgtcgcg ctccccgtgg tcaagacctt
     7141 catcggcaag gtgcgtgagc gcgccgtcgg tgaacaggtc atccgctcgg tcacgcccgg
     7201 ccagcaagtc gtcaagatcg tccacgacac gctggttgaa acgcttggcg tggaatcgga
     7261 agggctttcg ctcggcgcgc cgccggcttc catcctgatg gtgggtttgc aaggctccgg
     7321 caagaccacg acctcggcca agctcgccaa tctgctgcag actaaagaca agaagcgcgt
     7381 gctgatggcg tcgctggacg tcaaccgccc ggccgccatg gagcaattgc gggtgctggg
     7441 cgagcaggcc aatgtgccga cgcttcccgt ggtcctgggc cagcggccca ccgacatcgc
     7501 caggcgcgcc ttggcggcgg cccgcgtcgg cggctacgac gtgctgatcc tggataccgc
     7561 cggccgccag cacgtcgacg aagccctgat ggccgaagtc gccgaggtga aaaagctcac
     7621 caacccgcat gaaactctgc tggtggcgga cagcctcacc ggtcaggacg cggtcaatat
     7681 cgcgaaaagc ttcaacgagc gggtgcagat ctccggcatc atcctgacgc gcaccgatgg
     7741 cgatgcgcgc ggcggtgccg cgctttcgat gcgcgaagtc accggcgcgc cgatcaaatt
     7801 cctcggcacc ggcgagaagc tcgacgcatt ggaagtgttc cacccggcgc gggtggcatc
     7861 gcgcatcctg gacatgggtg atgtggtctc cctcgtcgag aaggcggtcg agcatgtcga
     7921 ccgcgagaag gccgaaaaac tcgccaaaaa gatgaaaaag ggcgagttcg atttcaacga
     7981 cctcgccgac cagctctccc agatgcgcaa gatgggcggc atgcaggggc ttttgggcat
     8041 gttgccgggt gtcggcaaga tcaaggatca gctcgccgcc gccggaatgg acgaccgggt
     8101 gctgaagcgg caggaggcga tcatctcctc catgacgcgc gaggaacgcg gcaagcccaa
     8161 tgtcatcaac ggttcgcgcc gcaaacgcat cgcggccggc gcgggcgtcg aggtgtcgga
     8221 cgtcaacaag atcctgaaga tgcaccgcca gatgtccgat gtgatgaaga agatgggcaa
     8281 aggcggccgg ggcatgctgg gtgcgctcgg cgggatgaat ccgaacatgc tggcgggatt
     8341 gggtggcggt ggcggcgcgg ggctgccgcc gggattttcc ggtctgccgc cgggcatgac
     8401 tcgcaaataa tctttttacg gacgaaaggg aatttcgatg gctttgaaaa tcagattgtc
     8461 gcgcggcggc accaagaaac gcccgcatta tgccgtggtg gtggcggaat cgcgcagccc
     8521 ccgcgacggg cgcttcatcg agcagatcgg cttttacaat ccgcttctgc ccaaggatca
     8581 tgccgaccgc ttgcggctcg atctcgacaa ggcgaaggcg tggctttcca aaggcgcggt
     8641 ggcatccgac cgcgtgcatc gtttcctgtc cgatgcgggc gtggtgaaag ccatcgtcca
     8701 tcatagtccc gagaagatga agcccaagaa gaaggcgcag gaacgcgccg ccgcggccgc
     8761 caaggccgcc ggtgcgggtg ctgcagaggc ttgacgcagt cccgcgtcct cctcggtgtg
     8821 gtcgccgcag cccatggcat caagggtgag gtgaaggtga agaccttcac cgaaagcccg
     8881 caagcgcttg gcgcctacgg gccggtgacg actgagcagg gacgcacgct tgagatcgtc
     8941 caattgcgcc ccaccaagcg tgacgaggcg gtggtgcgct ttgccgggct ctccgaccgc
     9001 aacgccgccg aagaattgag gggcgcgcag ctttctgtgc cgcgtgccgc attgcccccg
     9061 cctgaggacg gcgcatttta tcatgccgat cttgtcgggc tgcgcgccga agaccgttcg
     9121 ggctccgtgc tcggcaaagt gcttggggtg cacaatttcg gcgcgggcga cgtgctggaa
     9181 atcgaattca ccgccggggg cacggagttc gtcgccttcg ccgacccgca tgtgccggtg
     9241 gtggatgtcg cgggcgggcg tatcgtgatc gaactgccgc gtgagaacca gaactgaccc
     9301 aactcccttg tcatcctggg cgagccagcc ttag
//
LOCUS       DASYEG010000139         6126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2943046, whole genome shotgun sequence.
ACCESSION   DASYEG010000139 DASYEG010000000
VERSION     DASYEG010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2943046"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /gene="yihA"
                     /locus_tag="VGM72_06215"
     CDS             complement(<1..408)
                     /gene="yihA"
                     /locus_tag="VGM72_06215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEY4264894.1"
                     /translation="MTDEDTRAAQEEAEERGRILFAQDCDFLQGAANLDGIPATDLPE
                     VAFVGRSNVGKSSLVNALTGRKTLARVSNTPGRTREINFFRLGGRLILADLPGYGYAR
                     VSRTDSERWTELIFAYLRGRAHLRRAVLLIDSRR"
     gene            complement(431..2257)
                     /gene="yidC"
                     /locus_tag="VGM72_06220"
     CDS             complement(431..2257)
                     /gene="yidC"
                     /locus_tag="VGM72_06220"
                     /inference="COORDINATES: protein motif:HMM:NF002353.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEY4264895.1"
                     /translation="MSDQRNLLIAIVLSMAVVFGWQYFVGKPQIAQEQARQQQLAAEQ
                     KAKPAGAAGAAQPASGTAPATRNLPRDAALKLAPERADIDTPTLGGSINLTGGRFDDL
                     RLRAYHETPDPKSPEIELLSPMAAEHPYFAEFGWIADAAAQQKTPGPDTQWKLSKGDK
                     LSPGHDIELSYDNGSGLVFLRRIAVDTNYMFTIADTVENHGSQPVVLFPYGLVSRHNL
                     PPAQHYWVVHEGFIGVLDGTLKDPTYDDLGKNNDMQHFDSQGGWLGITDKYWMAAAIP
                     PQSEQFTATYKAYDANGVKAYQSDFTMKPKTVAPNGSQVVIHHLFAGAKVVDLVNDYG
                     KTLGIQRFDMAVDWGWFSFITKPMFLAIDFLYRYVGNFGVAILIFTVFVKALFFPLAN
                     ASYRSMSKMKKLQPEMERMRERFKDDKVQQQQEMMKLYQKEKVNPLAGCLPMVIQIPV
                     FFSLYKVLLVTIDMRHAPFFGWVKDLSAQDTSNVFNLFGLIPADPTQLPVIGHFLVLG
                     AFPMLMGLTMWLQTNLNPPPADPMQQKIFGFMPLIFMFMMANFPVGLVIYWSWNNTLS
                     IIQQVIIMKSTGTPVDFLDRLRGMAARLRRGEAPEKPAGPPD"
     gene            complement(2275..2550)
                     /gene="yidD"
                     /locus_tag="VGM72_06225"
     CDS             complement(2275..2550)
                     /gene="yidD"
                     /locus_tag="VGM72_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEY4264896.1"
                     /translation="MVDALRDLPRRAAIASLRAPILAYRAMISPLLPRACRFSPSCSA
                     YALEALTQHGPLKGSWLALRRLARCHPFTLLGGGCGYDPVPPHAHKP"
     gene            complement(2543..2944)
                     /gene="rnpA"
                     /locus_tag="VGM72_06230"
     CDS             complement(2543..2944)
                     /gene="rnpA"
                     /locus_tag="VGM72_06230"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEY4264897.1"
                     /translation="MRKRADFLRAQKGYRQPMPGLTLETCLSPPVLAKPRTARVGFTA
                     SRKVGGAVERNRAKRRLRAVAAATLPLSGRQGHDYVLIARTATLLRPFGELTRDLEKA
                     LRTAHAAIARAGTAPLPQRRGRRDPKEDHHG"
     gene            complement(3004..3138)
                     /gene="rpmH"
                     /locus_tag="VGM72_06235"
     CDS             complement(3004..3138)
                     /gene="rpmH"
                     /locus_tag="VGM72_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEY4264898.1"
                     /translation="MKRTYQPSKLVRKRRHGFRKRMSTKKGRQVIARRRAQGRKRLSA
                     "
     gene            complement(3278..4519)
                     /locus_tag="VGM72_06240"
     CDS             complement(3278..4519)
                     /locus_tag="VGM72_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264899.1"
                     /translation="MLKLSFVTAVAAAAIALPAFAAQTADPDVNKLPAGPEKAVVAEV
                     CTACHELGRIVYGNYNAAEWANNVHMMVNAGAALTPDQVDSVTKYLIKSFPERPANKF
                     VSVPGPVKVSIKEWELPIPGSRPHDPLAAPDGTMYFTAHMGSYIGHLDPKTGKFDIYK
                     TKTPISGPHGLVMDKDGNIWYTGNFKGYIGKLDVKTGKFTEFKTGAARDPHTPLFDKN
                     GILWFTSQNSNMVGRLDPKTGGIKLAVVPTEKAAPYGMVFDSKGKLWFSEWQAPKLAW
                     IDANTMEIHEITLPNPGTRPRRIAIDGNDVIWISDYSRGYLGMYDTKTGATKEWPSPG
                     GPKSEPYGITTLNGIVWYDEGNTKPNGLVRFDPKTEKFQTFPVLPSGGGVVRNMMPTK
                     DGTGLVMAESGVNHVAIATIN"
     gene            4904..>6126
                     /locus_tag="VGM72_06245"
     CDS             4904..>6126
                     /locus_tag="VGM72_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY4264900.1"
                     /translation="MHPDVMLKELKRPKPGWQLAVYSLSGRLLLLTIMFVMLSVALIY
                     FPSVARYHHQLLQDRIHSAQLVILPFTEAPGQQLSANLRKQLLERAGVDAVILRGGGQ
                     HELFLVGPDPPPIQAIYDGGETDLLDQIRDVVRALFAQPGRTIRIDAMTAIGEASSIV
                     VVANEEPIRAALFVFSARVLALSIFVSALTSLLVFVSLYLILVRPMQRITAAMVDFRN
                     NPEDPSRILHPSARRDEIGTAERELSSLQQELYGFLQQKTRLAGLGLAVAKIQHDLRN
                     ILSSAQIASDRLATLDDPVVKRVAPRLVDALDRAVNLATNTLRYGRAEERAPQRTLVA
                     LAPLIDEAASSALPEAGEIALEKRIPHGLEIDADSEQLFRVLLNLLRNARQALDAMPA
                     EENRRKHILVEARRKG"
BASE COUNT         1130 a         1872 c         1924 g         1200 t
ORIGIN      
        1 gcgccgtgaa tcgatcagga gcaccgcgcg gcgcaaatgg gcgcgccccc gcagataggc
       61 gaagatcagt tccgtccagc gctccgaatc ggtcctggag acgcgcgcat agccatagcc
      121 cggcaaatcc gccaggatca gccgcccgcc aagacggaag aaattgatct cgcgcgtgcg
      181 ccccggcgtg ttcgagacgc gcgcgagcgt cttgcgcccg gtcagcgcat tcacaaggct
      241 ggatttgccc acattggagc gtccgacaaa ggcgacctcc ggcaggtcgg tcgcggggat
      301 gccgtccaga ttcgcggcgc cctgcaggaa gtcgcaatcc tgcgcaaaga gaatgcgtcc
      361 gcgttcctcg gcctcttctt gtgccgcgcg tgtgtcctcg tccgtcatcg atggagcgtc
      421 aaatcgcgca tcaatccggc gggccggccg gcttttcggg agcttcgccg cggcgcagcc
      481 gcgccgccat gccgcgcagc cggtcgagga aatccaccgg cgtccccgtc gatttcatga
      541 tgatcacctg ctggatgatg gaaagcgtgt tgttccagct ccaatagatc accagcccga
      601 ccggaaaatt cgccatcatg aacatgaaga tcaacggcat gaagccgaag atcttttgct
      661 gcatgggatc ggcgggcgga ggattgagat tggtctggag ccacatggtc agacccatga
      721 gcatcggaaa ggcgccgagc acgaggaaat ggccgatcac cggaagctgc gtcggatcgg
      781 cgggaatgag cccgaacaga ttgaacacat tgctcgtgtc ttgcgcggaa aggtccttga
      841 cccagccgaa gaacggcgcg tgccgcatat cgatggtgac gagaagcacc ttgtacagcg
      901 agaaaaatac cgggatctgg atcaccatcg gcaagcagcc cgccagcgga ttcaccttct
      961 ccttctggta gagtttcatc atctcttgct gctgctggac tttgtcgtcc ttgaagcgct
     1021 cgcgcatgcg ctccatttcg ggctgcagtt tcttcatctt gctcatggag cggtaggacg
     1081 cattcgccag cgggaagaac agcgctttga cgaagacggt gaagatcaga attgcgacgc
     1141 cgaaattgcc gacgtagcga tagagaaaat cgatcgccag gaacatcggt ttggtaatga
     1201 aggagaacca gccccaatcc accgccatgt cgaaacgctg gatgcccaga gtcttgccgt
     1261 aatcattgac gagatcgacg accttggcgc ccgcgaaaag atgatgaatc acaacctgcg
     1321 agccgttcgg cgcgaccgtc ttcggcttca tggtgaagtc ggattgatac gccttcactc
     1381 cgttggcgtc ataggccttg taggtcgcgg tgaattgctc cgactgcgga ggaattgccg
     1441 cagccatcca atatttgtcg gtgattccga gccagccgcc ctggctgtcg aaatgttgca
     1501 tgtcgttgtt cttgccgaga tcgtcatagg tcgggtcttt cagcgtgccg tcgagcacgc
     1561 cgatgaagcc ctcatgcacc acccaataat gctgcgcggg tggaagattg tggcgcgaga
     1621 cgagtccata cggaaaaagc accaccggct ggctgccgtg attttccacc gtatcggcga
     1681 tggtgaacat gtagttggtg tcgaccgcaa tgcggcgcag gaagaccaga ccggacccgt
     1741 tgtcgtagga aagctcgatg tcgtgacccg gcgagagttt gtcgcccttg ctgagtttcc
     1801 attgcgtgtc gggaccgggc gtcttttgct gcgcggccgc atcggcgatc cagccgaact
     1861 ccgcgaaata gggatgctcg gccgccatcg gcgagagcag ctcgatttcc ggactcttgg
     1921 gatcgggtgt ctcgtgatag gcgcgcaggc gcaaatcgtc gaagcgccct cccgtcagat
     1981 tgatggagcc gccgagcgtg ggcgtatcga tgtccgcgcg ctccggcgcc agcttgaggg
     2041 cggcatcgcg cggaagatta cgcgtggcgg gagcggtccc ggaggccggt tgcgccgcgc
     2101 ccgctgcgcc cgccggtttg gctttttgct cggcggcgag ctgctgctgg cgcgcctgct
     2161 cctgggcgat ctgcggcttg ccgacgaaat attgccatcc gaagaccacg gccatcgaca
     2221 acacgatggc gattagcaga ttgcgctggt cggacatggg gactccggtg ggccttaagg
     2281 cttgtgagcg tgcggcggga caggatcata gccgcagccg ccgccgagca gtgtgaaggg
     2341 atggcaacgt gcaagcctgc gaagcgcaag ccaacttccc ttgagcgggc catgctgggt
     2401 cagcgcctcg agcgcatagg ccgagcagga tgggctgaag cggcaggccc gcggcagcag
     2461 cggcgaaatc atggcgcgat aggcgaggat gggcgcgcgc aaggacgcaa tggcggcgcg
     2521 gcgcggcaga tcgcgcagag catcaaccat gatggtcctc ctttgggtcg cgccggccgc
     2581 gtctctgcgg caggggcgct gttccggcgc gagcaatcgc ggcatgggcg gtcctgagcg
     2641 ctttctccaa atcccgggta agctcgccaa agggacgcaa aagcgtcgcc gttcgggcga
     2701 tcagaacata atcatgcccc tggcgccctg aaaggggcag ggtcgcggcg gccactgcgc
     2761 gcaaacgccg cttggcgcga ttgcgctcca cggctccccc cactttgcgg ctcgcggtga
     2821 agccgacccg cgcggtcctg ggtttggcga gaacgggcgg cgaaagacag gtctcgagcg
     2881 tcaggccggg catgggctgg cgatacccct tttgcgcccg caggaaatcg gcccgttttc
     2941 tcatgtggtc aagggcaagc ttggcggaaa gcccagattc gggggacggc tcggcgccgg
     3001 cgttcaggcc gatagcctct tgcggccttg ggcgcgacgg cgggcgatca cttggcgccc
     3061 cttcttcgtc gacatgcgtt tgcgaaagcc gtgacggcgc ttgcggacga gtttgctcgg
     3121 ctgataggtg cgcttcacgg caagtctccg gaaaaaaaag ccgggtttgg ctcgaaagag
     3181 ccgtgcttct acggggccga ctggccccct gtcaaccaaa agcggcggcg tttcccccgc
     3241 cgcttttgcg ccaaaccgcg aaacctctcc tgcccgatca gttgatcgtg gcgatggcga
     3301 cgtgattgac gccgctttcc gccatcacaa gacccgtccc gtcctttgtg ggcatcatgt
     3361 tgcgcacaac gccgccgccg gacggaagca ccgggaacgt ctggaatttc tccgtcttgg
     3421 ggtcgaagcg caccaggccg ttcggcttcg tattgccttc gtcgtaccag acgatgccgt
     3481 tcagcgtggt gatgccatag ggctccgatt tcggaccgcc cggcgagggc cattccttgg
     3541 tggcgcccgt cttggtgtcg tacatgccga gatagccgcg cgaataatcc gaaatccaga
     3601 tcacgtcatt gccgtcgatg gcgatgcgcc gcgggcgggt tccgggattg ggaagcgtga
     3661 tttcatggat ttccatggtg ttggcatcga tccaggcaag cttgggagcc tgccattcgc
     3721 tgaaccagag tttgcccttg ctgtcgaaca ccatgccgta aggcgcagcc ttttcggtcg
     3781 gcaccacagc gagcttgatc ccgcctgtct tcgggtcgag ccggccgacc atgttgctgt
     3841 tctgggaggt gaaccaaagg atgccgttct tgtcgaacag cggcgtgtgc ggatcgcgtg
     3901 ccgcgccggt cttaaattcc gtgaattttc cggtcttgac gtcgagcttg ccgatgtagc
     3961 ccttgaagtt gcccgtgtac caaatgttcc catccttatc catcaccagc ccgtggggac
     4021 ccgagatggg ggtcttcgtc ttgtagatgt cgaacttgcc ggttttcgga tcgagatgac
     4081 cgatatagct tcccatatgg gcggtgaaat acatggtgcc gtcgggagcc gcgagaggat
     4141 cgtgcggccg ggaacccgga atcggcagtt cccattcctt gatcgagacc ttcaccgggc
     4201 ccggtacgga gacgaacttg ttggcgggac gctccggaaa gctcttgatg agatatttgg
     4261 tgaccgaatc gacctggtcc ggcgtgaggg ccgctcccgc attgaccatc atgtggacgt
     4321 tattggccca ttcggccgcg ttgtaattgc cgtatacgat ccggccgagt tcatggcagg
     4381 ccgtgcagac ttccgccacc acggcttttt cgggccctgc tggaagcttg ttgacgtcgg
     4441 gatcggcggt ttgcgccgca aaggcgggca gcgcaatagc cgcagccgcg acagcggtca
     4501 cgaaggacag tttcagcata gggtacccct tgcgaatttg gttcggctta agcggccgca
     4561 atttaatgca accctagggg tctgccaata gcacagggcc agaaccagca gtacaaccca
     4621 gacccagcgg gtcaagggtt cgtgaccggg cttcgtcgcc gagacggcaa gacaggcgct
     4681 ccaggcccag gagcttcaga cccaggagcc tcatgcgacg tcgctccggg ccaaggcacc
     4741 tcccaacgcc ggacatgccg atgatgatcc gcgtcgatcc gccctcccat tttccgcggg
     4801 gacacatata ttctgtttgg acacaacgcc gaaaatcgtc ccagtcttcc cgcggaccgg
     4861 ccagcgaaaa atttgccttt ggcatgattt cgccggaaat ctggtgcacc ctgatgtcat
     4921 gttgaaggag ctgaaaaggc cgaaacccgg gtggcagctg gccgtctaca gcctttcggg
     4981 ccggcttctg ctgctgacca tcatgttcgt gatgctgagc gtcgcgctga tctattttcc
     5041 ctccgtcgcg cgctatcatc atcaattgct gcaggaccgg attcattccg cccagctcgt
     5101 catcctgccg ttcaccgaag cgcccggcca gcaattgtcg gccaatctgc gcaagcagct
     5161 tctggaacgc gcaggcgtcg atgccgtgat cctgcgcggc ggaggccagc acgaactttt
     5221 cctcgtcggt cccgacccgc cccccatcca ggcgatctat gacggcggcg aaaccgatct
     5281 gctcgaccag atccgcgatg tcgtgcgtgc gttgttcgcc caacccggcc gcaccattcg
     5341 catcgatgcg atgaccgcca tcggcgaagc cagcagcatc gtcgtggtgg ccaacgagga
     5401 accgatccgc gccgctcttt tcgtgttttc cgcgcgtgtg ctggcgcttt cgatcttcgt
     5461 ttcggcgctg acttcgcttc tggtgttcgt gagtctttac ctgatcctgg tgcggccgat
     5521 gcagcgcatc accgcagcga tggtcgattt ccgcaacaat ccggaagatc cctcgcgcat
     5581 ccttcatccg tccgcgcgcc gcgatgagat cggcacggcc gaacgcgagc tttcttcact
     5641 ccagcaggag ctctacggct tcttgcagca gaagacgcgg ctcgcgggcc tcgggcttgc
     5701 ggtcgcaaaa attcagcatg atttgcgcaa catcctttcg agcgctcaga tcgcctcgga
     5761 tcgccttgcc acgctcgacg atccggtggt caagcgtgtg gcgccccgcc tcgtggacgc
     5821 gctcgaccgc gcggtcaatc ttgccaccaa cacgctgcgc tatgggcgcg ccgaagaacg
     5881 cgccccgcag cggacgctcg tggccctggc gcccctgatc gacgaggcgg cgtcctcggc
     5941 cctgcctgaa gccggcgaaa tcgcgctgga aaaacgcata ccgcacggac ttgagatcga
     6001 tgccgattcc gaacagctct ttcgcgtgtt gctcaacctg ttgcgcaacg cgcgtcaggc
     6061 gctcgatgcg atgccggccg aggaaaatcg gcgcaagcac atcctggtcg aggccaggcg
     6121 aaaggg
//
LOCUS       DASYEG010000140         3201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3088799, whole genome shotgun sequence.
ACCESSION   DASYEG010000140 DASYEG010000000
VERSION     DASYEG010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3088799"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1727)
                     /locus_tag="VGM72_06250"
     CDS             complement(<1..1727)
                     /locus_tag="VGM72_06250"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY4264901.1"
                     /translation="MGEERPAFKAQAEQNHAAGNCLHRAAAGTGAFFAREAERTRAFV
                     TSLSWPADWRQQIRWPVILFGSLAGVTLVGAAALFLVMSAVAPVIPAHADLYALNRPQ
                     ALTFTDETGQVVGVRGAIVGERLKLSDMPPYLPAAFLAMEDRKFYRHHGIDLNGLMRA
                     AMVDLKARRIVQGGSTITQQVVKIVLLSPDRTFSRKLTEIAGAFALERRLTKDQILEL
                     YLNRLYLGSGAYGVDGAAHVYFGKSARDVTLSQAAMLAALTRAPTAFSPRRDLAAAQA
                     RANRVLGAMVASGTLMPEEAARARATPATVIDPTQNLARDYYLDAAADEVKQILPHTA
                     GDLTIVTAMDSALQETARSQIVTVLDKRSSKNAHATQAALVSMTPQGAVRALIGGKDY
                     AESAFNRVTQAHRQPGSAFKPFVYLAALEHGLTPATVRMDEPITIKNWSPDNYDDEHL
                     GPVTLEQAFAQSINTVAVGLGQEAGLTQVVAAAHRLGIRSALQPVASLALGTSEVTPL
                     ELTGAYASFASMGKQVRPYMVVSVRSPNGATLYRHDVPPSAPLFSEDEGLAMNAMMYE
                     VVQTGTGRAA"
     gene            1935..2381
                     /locus_tag="VGM72_06255"
     CDS             1935..2381
                     /locus_tag="VGM72_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264902.1"
                     /translation="MATSSKSPKKQTRKKHTDAEAAAESVGSVVRRIIADESPVLEDW
                     PENWPENWTADLIRRVLAWQIEQMEMKPSKSPKIAAQRARDARTMTELVRTLEKLDAV
                     NRRRESKGRKTKPRDDKAIKEQFIRRLDQLLAARVEGPAAGKPESG"
     gene            2344..>3201
                     /locus_tag="VGM72_06260"
     CDS             2344..>3201
                     /locus_tag="VGM72_06260"
                     /inference="COORDINATES: protein motif:HMM:NF015211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HEY4264903.1"
                     /translation="MRARLLESLSPADAEFLNSDWPFWARPEQLAPEGDWRIWLFLGG
                     RGAGKTRAGAEWILDGVREGAMRRVALVGATYADVRDVMIAGESGLLAAGRAEGLRYE
                     ASKRQVHWPSGAVAQAFTAEEPDGLRGHQFDGAWLDEFCKARDPETTLDMLLMALRIG
                     ENPRALLTTTPRNIAAFKALLAMPAVAVTRAGTFANAANLAPAFLEQMRLQYAGTRLG
                     RQELDAELIEDNESALWRREWIEAARVREWPALRRVVVAVDPPASAGADADECGIVIA
                     GASADGHGYV"
BASE COUNT          595 a          986 c         1083 g          537 t
ORIGIN      
        1 gcggcacgcc cggtgccggt ctgcacgacc tcatacatca tggcgttcat cgcgagcccc
       61 tcgtcttcgg aaaaaagcgg tgcggacggc ggcacatcgt ggcggtagag ggtggcgccg
      121 ttgggcgagc gcaccgagac caccatatag gggcggacct gcttgcccat cgatgcgaaa
      181 ctggcatagg cgcctgtcag ttcgagcggc gtgacttccg atgtgcccaa cgccagggag
      241 gcaaccggct ggagcgcaga ccggatgccc agtctgtggg ccgcagcgac gacctgtgtt
      301 agcccggcct cctggccgag gccgaccgcc acggtgttta tggattgcgc gaaggcctgt
      361 tccagagtca ccggtcccag atgttcgtcg tcataattgt ccggcgacca gttcttgatg
      421 gtgatgggtt cgtccatgcg cacggtcgcg ggcgtcagcc catgttcgag agcggcgaga
      481 tagacgaaag gcttgaaggc ggaccccggc tgacgatgcg cctgggtgac gcgattgaac
      541 gcgctttcgg cgtagtcctt acccccgatc agcgcgcgca cggcgccctg cggcgtcatg
      601 gagacaagtg ctgcctgcgt cgcatgcgcg ttcttcgagc tgcgcttatc gaggacggtg
      661 acgatctggg aacgcgcggt ttcctgcaac gcggaatcca tcgcggtgac gatagtgaga
      721 tcgccggccg tgtgcggcag gatttgcttc acctcgtccg ctgccgcgtc gagataataa
      781 tcgcgcgcca gattctgcgt tggatcgatc acggtggcgg gcgtcgcgcg agcccgggcg
      841 gcctcttccg gcatcaatgt gccgctcgca accatggcgc cgagcacgcg attcgcacgg
      901 gcctgcgcgg cggcaaggtc gcgccgcggc gagaacgccg tgggcgcgcg cgtcagtgcc
      961 gccagcatcg cggcctggga cagagtcacg tcgcgcgccg atttgccgaa atagacatgg
     1021 gctgcgccgt cgacgccata ggcgccggag ccgagataga gccggttcaa atacagctcc
     1081 aggatctggt ctttcgtgag ccggcgttcg agtgcgaacg cgccggcgat ttcggtcagc
     1141 ttgcgggaga aggtgcggtc cggcgacagc aacacgatct tcacgacctg ctgcgtgatc
     1201 gtggaaccgc cctgcacgat gcgccgggct ttcaaatcga ccatcgccgc acgcatcagc
     1261 ccattcagat cgatgccgtg atggcgatag aatttgcggt cttccatggc gaggaacgcg
     1321 gccggcagat agggcggcat gtcggagagc ttcagccttt cgccgacgat cgcgccacgc
     1381 acccccacca cctgtccggt ttcatcggtg aaggtgaggg cttgcggacg attgagcgca
     1441 taaagatcgg cgtgcgcggg aatgaccggc gcgacggcgg acatcacaag gaacagcgcg
     1501 gcggcgccca caagtgtcac accggccagg ctgccgaaca ggatgaccgg ccagcgaatc
     1561 tgttggcgcc aatcggcggg ccaggagagg ctggtcacga aggcacgggt ccgctccgct
     1621 tcacgcgcga agaaagcccc cgttcccgcc gccgcccgat ggagacaatt gccggccgcg
     1681 tgattttgtt ccgcttgcgc cttgaaagcg ggacgctcct cgcccatctg ggtcctatct
     1741 ccgaaaatct atgctgggaa acgcgccaac gggatcaacg tcccgtgacc gctggaacaa
     1801 tttactttgg cgaatccgca acgaaatcgg gtgatacgca ttggcgaatt gcggcgggat
     1861 cggggcagca tgccgcgctc atgcccaagc caatccgata aattccgcgc cgcgtcttga
     1921 agggggacgc tgtcatggcc acttcgtcca aatccccgaa aaagcaaacc cggaagaaac
     1981 acacggacgc cgaagccgcc gccgagtcgg ttggctctgt cgtcaggcgg atcatcgccg
     2041 atgaatcgcc ggttcttgaa gattggccgg aaaattggcc tgaaaattgg acagccgatc
     2101 tgatccggcg tgttcttgcg tggcagatcg agcagatgga aatgaaaccc tccaagagtc
     2161 caaaaatcgc agcccagcgc gcccgcgacg cgcgcaccat gaccgagctt gtccgcacgc
     2221 tcgaaaaact cgacgcggtg aaccggcgcc gcgaatccaa agggcgtaaa acgaaaccgc
     2281 gcgatgacaa agccatcaag gaacaattta tccgccgcct cgatcaactg cttgccgccc
     2341 gagttgaggg cccggctgct ggaaagcctg agtccggctg acgcagaatt cctgaacagc
     2401 gattggccgt tctgggcgcg ccccgaacaa ctcgcgccgg agggcgattg gcgcatctgg
     2461 ttgtttctgg gcgggcgcgg cgcaggcaaa acgcgcgccg gcgcggaatg gattctggat
     2521 ggggtgcgtg agggcgcgat gcggcgcgtg gcgctggtgg gcgccaccta tgccgatgtg
     2581 cgcgacgtca tgatcgcggg cgaatcggga ttgctggcgg cgggccgggc cgagggcttg
     2641 cgctatgaag cctccaagcg gcaggtgcat tggccgagcg gcgcggtggc gcaggccttc
     2701 acggccgagg agccggacgg tctgcgcggc catcaattcg atggcgcctg gctggatgaa
     2761 ttctgcaaag cgcgcgatcc cgaaacgacg ctcgacatgc tcctgatggc tttgcgcatc
     2821 ggggaaaatc cgcgtgcgct tctgaccacg acgccgcgca acatcgccgc gttcaaggcg
     2881 ctgctggcga tgcccgcagt ggcggtgaca cgcgcgggca cctttgccaa cgcggccaat
     2941 ctggcgcccg cgttcctgga acagatgcgt ctgcaatatg ccggcacgcg tctggggcgg
     3001 caggaattgg acgccgaact gatcgaggac aatgaatccg cgctgtggcg gcgcgaatgg
     3061 atcgaggctg cgcgcgtgcg cgaatggccc gcgctcagac gcgtcgtcgt tgccgtcgat
     3121 ccgcccgcat cggccggcgc ggacgcggat gaatgcggca ttgtcatcgc gggcgcgagc
     3181 gcggacggcc acggctatgt g
//
LOCUS       DASYEG010000141         2658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3958527, whole genome shotgun sequence.
ACCESSION   DASYEG010000141 DASYEG010000000
VERSION     DASYEG010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3958527"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VGM72_06265"
     CDS             <1..379
                     /locus_tag="VGM72_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264904.1"
                     /translation="AKEDHIAPAASVYKGAKLFGGPVTYMLAGSGHIAGVINAPAAGK
                     YQHWTNAALPATLGEWQAGATEHAGSWWPEWDAWLAPRSGEKIPAPKPGDGELKSLGD
                     APGTYVLVKAGTMNQPKKARASH"
     gene            430..897
                     /locus_tag="VGM72_06270"
     CDS             430..897
                     /locus_tag="VGM72_06270"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY4264905.1"
                     /translation="MKRTLVCSLAAMALLGAAMQTGARAQDSAFFGAMMAPLTPNIQH
                     GLAIEISKGAAIGDIVAGSPAAAAGLRGDDVIIDIDGQPVDSPQDVIAVIERHRPGDR
                     IAVQILDSRNGHRANTVSVTLAARPSGFHPDQQPGPQPVPASGRPKSDLTPPE"
     gene            complement(881..1918)
                     /locus_tag="VGM72_06275"
     CDS             complement(881..1918)
                     /locus_tag="VGM72_06275"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEY4264906.1"
                     /translation="MEFIDLRSDTVTRPSAGMRRAMAEAPVGDDQYGDDPSINLLQER
                     VAQLLGKEAALFVPSGTMANQIGLKLLTRPGDDVILGHETHMVWHESGAGAANAGVQF
                     SPIGTEGLFTPAEFRSHVKPHGHMMLPPTTLVVIENTHNRCGGLVFPQNDAVAICDAA
                     RALGIRTYLDGARLFNAAAASGRSVAQLSAPFELVSVALSKGLGCPVGSVVAGSKADI
                     ARAVRLRRMFGGAMRQAGILAAAGLYALDHNLARLPEDHAHARLMAERLTALRGVRLD
                     LATVQSNIVIFHMEENAPDAATIAVRAKDAGVLVSVFGARKLRAVTHLDVSRADCENA
                     AEILARVIQAE"
     gene            complement(1918..2544)
                     /locus_tag="VGM72_06280"
     CDS             complement(1918..2544)
                     /locus_tag="VGM72_06280"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEY4264907.1"
                     /translation="MYVPAHFALSDIHGFLRSHVFAVIAGEIGGTIHFAYAPVVLDRG
                     LGPLGAIRFHLARANPLANMETGVALKISVMGPHAYISPDWYETDAMVPTWNYLAVEG
                     AGPVRRLGDFELRRLLSDLAAQEESALAPKPQWKMGRVPPARLDVLLTAIIGFELIFE
                     TLEGKAKLSQNRAPADVEGAIGGLERRGDAGSLAVAAAMRKARGGEGA"
BASE COUNT          541 a          874 c          798 g          445 t
ORIGIN      
        1 ggcgaaggaa gaccacatcg caccggccgc gtccgtctac aaaggcgcga aactgtttgg
       61 cggacccgtg acctacatgc tcgcgggttc cggtcacatc gccggtgtca tcaatgcgcc
      121 cgcggccgga aaatatcagc actggaccaa tgccgccctg cccgccaccc ttggcgaatg
      181 gcaagcaggc gcaaccgagc atgccgggtc ctggtggccg gaatgggacg cgtggctcgc
      241 accgcgctcg ggcgagaaaa tccccgcgcc caaacccgga gatggcgagt tgaaatctct
      301 gggcgacgcg ccgggcacct atgtgctcgt aaaagcgggc accatgaatc agcctaaaaa
      361 ggcgcgcgcc agccactagg aattattagg tgttgctcct ctatcatggc tccggttccg
      421 gaggccgcca tgaaaaggac gcttgtttgc tcgctcgctg ccatggcgct gctgggggcg
      481 gcgatgcaga caggcgcccg ggcacaggac agtgccttct tcggcgccat gatggcgccg
      541 ctgacgccga atattcagca cggccttgca atcgaaatca gtaagggcgc ggccatcggc
      601 gatatcgtcg cgggcagtcc cgcggccgcc gccggtctgc ggggtgatga cgtcatcatc
      661 gacatcgatg ggcagccggt ggattcgccg caagatgtca tcgccgtcat cgaacgccac
      721 aggcctgggg accggattgc ggtgcagatt cttgattcac gcaatggtca ccgcgccaat
      781 acggtcagcg tgacattggc cgcgcgccct tcgggatttc acccggacca gcagcctggt
      841 ccccagccag taccagcctc aggccgtcca aaatccgatc tcactccgcc tgaataacgc
      901 gcgccaaaat ctcggcagca ttctcgcaat ccgcgcggct gacatcgaga tgggtcaccg
      961 cgcgcagctt gcgcgcgccg aacaccgaca caagaacgcc cgcatccttg gcgcggaccg
     1021 cgatggtcgc agcgtcggga gcgttctctt ccatatggaa aataacgatg ttgctttgca
     1081 ccgttgcaag atcgaggcgg acgccccgga gcgccgtcag acgttcggcc atcagacgcg
     1141 catgcgcatg atcttcgggc aagcgcgcga gattatgatc gagcgcataa agtccggccg
     1201 cggccagaat gccggcctgc cgcatggcgc caccgaacat gcgccgaagc cggaccgcgc
     1261 gcgcgatatc ggcctttgaa cccgccacga cgctgccgac cgggcaaccc aaacctttgg
     1321 acagcgcgac agagaccaat tcgaagggag ccgagagttg ggcaacgctg cgccctgaag
     1381 cggccgccgc attgaacagg cgcgcgccat cgagataggt gcgaatgccg agggcgcgcg
     1441 cggcatcgca gatcgcaacc gcgtcgtttt gcggaaacac cagcccgcca cagcgattgt
     1501 gcgtgttctc gatcaccacc agcgtcgtcg gcggcaacat catatggccg tgcggcttca
     1561 catgactgcg aaattcggcc ggagtgaata gaccttcggt cccgatgggc gagaactgca
     1621 cacccgcatt ggcggcgccg gcgcccgatt catgccacac catatgcgtt tcatggccca
     1681 ggatcacgtc atcgccaggc cgcgtcagaa gcttgagacc gatctggttc gccatagtgc
     1741 cgctcggcac gaacagcgcg gcttccttgc ccagcaattg ggcgacacgt tcctgcagca
     1801 gattgatgct gggatcatcg ccatattgat cgtcgccgac cggggcttcc gccatggcgc
     1861 ggcgcatccc ggcggacggc cgggtgacgg tgtcagatct gagatcgata aattccatta
     1921 ggccccctcg cctcctcgcg cctttcgcat tgccgcggcc acagcgaggc ttcccgcatc
     1981 gccgcggcgt tcaaggcccc caatggcacc ttccacatcg gcgggggcgc ggttctgcga
     2041 cagttttgct tttccttcga gtgtttcgaa aatcagttca aacccgataa tggcggtcag
     2101 aagcacgtcc agccgcgccg gcggcacacg ccccatcttc cactgcggtt ttggcgccaa
     2161 agcggattct tcctgtgcgg ccagatcgga aagcagccgc cgcaactcaa aatcgccaag
     2221 acgccgcaca ggaccagcac cttcgaccgc gagataattc caggtcggga ccatcgcatc
     2281 ggtttcatac cagtcgggtg agatataagc gtgcggtccc atcacggaga ttttcagcgc
     2341 aacgccggtt tccatgttcg ccaagggatt ggcgcgggcg agatgaaaac gtatggcgcc
     2401 aagcggaccc agcccccgat cgagaacgac gggcgcgtag gcgaagtgaa tcgtcccacc
     2461 gatctcgccc gcgatgaccg cgaacacatg cgaacgcaag aaaccgtgga tgtccgaaag
     2521 cgcgaaatgg gccggaacat acatggttca aacaagcccc gccgtttccg gccagcccat
     2581 catcacattg agattctgca cgcaagcccc ggcagcgcct ttcccgagat tgtcgagcac
     2641 cgccaccacg cgcgcctg
//
LOCUS       DASYEG010000142        28713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3089166, whole genome shotgun sequence.
ACCESSION   DASYEG010000142 DASYEG010000000
VERSION     DASYEG010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 28713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28713
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3089166"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VGM72_06285"
     CDS             <1..339
                     /locus_tag="VGM72_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264908.1"
                     /translation="AISNDDMLWYGDYTRGYLGMYDPRTGKAKEWKSPAGPDSAPYGI
                     TTTPGAIWYSESGANPNTLVRFDRKSEKFQTWKIPSGGGTVRNMMATRDGNLAIAESG
                     ENVIALVEIK"
     gene            complement(354..1331)
                     /locus_tag="VGM72_06290"
     CDS             complement(354..1331)
                     /locus_tag="VGM72_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4264909.1"
                     /translation="MDPKSPFVSFAPNNVISANAQYRPIFVWFSSARQACPHILKRKQ
                     DMPMLTTKDDTEIFYKDWGSGTPLFFHHGWPLSADDWDAQMMFFLGKGYRVIAHDRRG
                     HGRSTQTANGHDMDTYAADVAELTKALDIKGAVHIGHSTGGGEVVRYVAKYGTGRVAK
                     AVLISAIPPALMKTDTNPEGLPKEVFDGLRDGTANHRSQFYKDITMPFYGFNRAGAVI
                     SEGIRENWWRQGMMGAIKAQYDCVKVLSETEFFDDLKAIDVPVLVMHGEDDQICPFPA
                     SGARTIKLLKHGSLKTYPGFPHGMPTTHAEIINADLLAFIASKENFRKS"
     gene            1313..2311
                     /locus_tag="VGM72_06295"
     CDS             1313..2311
                     /locus_tag="VGM72_06295"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY4264910.1"
                     /translation="MAIWDPPTEIGILLYPEVASATVHGLTDLFTVATTLVREHLGPT
                     APTLRISHWQPGSTNNAIECVFDTHPQFATRPVSVIVPGSWKGQPASEVRRRLVQWLI
                     ERHAAGTTLCSVCGGAFILAETGLLAGRSVTTHWSLKGELADRFPEIQVDESRLTIED
                     GNFITAGGVLAWADLGLKLVDRYLGPTIMLRTSQFMLADPPGREQRYYSHFSPKLHHG
                     DSSILKVQHWLQTQATKKPSVSEMAKAGGLEPRTLHRRFQKATGLKPNEYSQHLRVGK
                     AREMLEFTNRTVDQVASAVGYEDPSSFRRVFQRVVGLSPGAYRRRFAAANAARTAT"
     gene            complement(2389..3879)
                     /locus_tag="VGM72_06300"
     CDS             complement(2389..3879)
                     /locus_tag="VGM72_06300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEY4264911.1"
                     /translation="MSGEILTIAQMGEADLLSVEGGIPSLTLMENAGDAVANAVTAHH
                     PNAAALVLCGPGNNGGDGFCAAAKLRERGVEVRVACLSPPASLKGDAAAMANRWGGPI
                     VPFDEAVLEDASLIVDALFGAGLARPLQGIVADVVRRANASGIPILAVDVPSGLHGDL
                     GRPLDGAGGVCIHAAQTVTFFRRKAAHLLFPGRGLCGDIILADIGIPEGVLETIRPRS
                     FENTPELWRASFPTIRRAGHKYDRGHAVVVSGPMHATGAARLAARAALRIGAGLVSVA
                     SPLDAVGVNAAQLTAIMVKPFEGAKGLSALLADKRLNAVAIGPGCGVGPRTQDLVAAV
                     LACGAAAAIDADGLTSFGDDPNALFRQLDAGMVLTPHEGEFSRIFPDLLRNAPSRLDA
                     TREAARIAGCVVLLKGPDTVIAAPDGIAAINANAPPWLATAGSGDCLTGMIAGLMAQG
                     MGAFEAACAGVWLHGDAANRYGPGLIAEDIPEQLPAVLKGCGMTSC"
     gene            complement(3889..5061)
                     /locus_tag="VGM72_06305"
     CDS             complement(3889..5061)
                     /locus_tag="VGM72_06305"
                     /inference="COORDINATES: protein motif:HMM:NF006488.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEY4264912.1"
                     /translation="MTSRNLNPVFEGLGTTIFTVMSALAGAHGAINLGQGFPDEDGPT
                     GIREAAARALRDGPNQYPPMQGLPLLRQAVAGHAKRFYDLAFDPETEVLITSGATEAL
                     TDCVMALIAPGDEAVLIEPCYDSYPPMLAAMGARVRSLRLATPDFRLTEGALAAVFSE
                     RTKLIIVNSPLNPIGRVFDRAELELLAQFLKHYDAYAVCDEVYEHLVFDGRAHVPLIS
                     LPGMRERCVRVGSAGKMFSLTGWKIGWICGPARLIQVISKAHQFNTFTTSPALQLGVA
                     HGLEHEMDYALENTRALQVKRDSIAGTLQAVGFDVLSSEGTYFITASIRGLTNETDRE
                     FCKRITREAGITPIPLSVFYSEGGPADLVRFAFCKKRAVLEDAGKRLNNYFSALAG"
     gene            5291..5629
                     /locus_tag="VGM72_06310"
     CDS             5291..5629
                     /locus_tag="VGM72_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018900556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY4264913.1"
                     /translation="MKKVEAIIKPFKLDEVKEALHEVGVQGITVTEAKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEIVLMPEQVEPAVEAILKAARTGRIGDGKIFILDVEEAVRI
                     RTGEKGADAI"
     gene            5689..7098
                     /gene="glnA"
                     /locus_tag="VGM72_06315"
     CDS             5689..7098
                     /gene="glnA"
                     /locus_tag="VGM72_06315"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018678638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEY4264914.1"
                     /translation="MADAKSVLKMIKDKEVKFVDLRFTDPRGKWQHVTFDLSMIDESL
                     FSQGTMFDGSSIAGWKAINESDMVLMPDPDSAVIDPFFAQTTLAINCDIHEPLTGQPY
                     NRCPRSISKAAEKYLASAGIGDTAYFGPEAEFFIFDDVRFDVGMNKGYFFLDALEGAY
                     NTGTEYESGNLGHRPPVKGGYFPVPPVDSAQDIRSEMLAIMGDMGIQPEKHHHEVATA
                     QHELGFKFNTLTKCGDNMQIYKYVIHQVAASYGKSATFMPKPIYGDNGSGMHVHQSIW
                     KEGKPLFAGSGYADLSELCLYYIGGIIKHAKALNGFTNPLTNSYKRLIPGFEAPVLLA
                     YSARNRSASCRIPFGAGPKAKRVEVRFPDPGANPYLAYASMLMAGLDGIENKIHPGEP
                     MDKDLYALPPEELKDIPQVCGSLREALSHLDADRAFLKKGGVFTDDFIDSYIELKMGE
                     VYAFEHTPHPVEFKMYYSV"
     gene            complement(7184..8620)
                     /locus_tag="VGM72_06320"
     CDS             complement(7184..8620)
                     /locus_tag="VGM72_06320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY4264915.1"
                     /translation="MMQARTASNAPDPRILKVQVERAANAIYGAIAGSPLWVCFFALM
                     CSDPMPFLGKVSALNALALVLVVTLTAGVATLVLRTFSRCRESAVDAVASKRWRSRLL
                     WMSAGLSAAWGCAPWLLWDEVNTTNHVFIELMCLAIVGRFLVIRANHIPFFLASYLPM
                     GSMLFLRCVASQQMTDMVLASLVPLYAIQVFLDSVHISDRWDDEALIRFSHEDLSREL
                     EETRDEALMKRAEAESANTSKTAFLANMSHELRTPLNAILGFSEIIARECLGAVGNPR
                     YKEYAGDIHNSGAHLLSLINDLLDVAKIESGRMEIEPQVIETRRTLDGALKFVSARAR
                     ERGQTLSISVADDAAILYADERALKQIVINLVSNAVKFTHDGGHIEMTAKRNNTGDFD
                     LTVADNGPGIPKDKIGKIFKPFSRVDNRYDSGNSGTGLGLALVRGLAELHGGRAWLES
                     EEGGGTRVHVVLPMTAMVQERPKRRLSA"
     gene            8800..10797
                     /gene="parE"
                     /locus_tag="VGM72_06325"
     CDS             8800..10797
                     /gene="parE"
                     /locus_tag="VGM72_06325"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEY4264916.1"
                     /translation="MAKIRDLFEEPAEPAALSSRGPADKSYTAKDIEVLEGLEPVRRR
                     PGMYIGGTDERALHHLAAEIMDNAMDEAVAGYANRIEIELKPDDILIVRDNGRGIPID
                     PHPKFKNKSALEVIMTTLHAGGKFGGKVYDTSGGLHGVGASVVNALSEWIEVEVARDR
                     RTYKQRFERGHPAGKLKDLGPAPNRRGTIVSFKPDIKIFGTANFSPRVLYRMARSKAY
                     LFRGVEIRWKCDSSLLKPEWEIPVEDVLKFPGGLLDFLKGEISASKTVTRPFFGQIQN
                     RDGKGAVEWAIAWSPQIDPFVHSYCNTIPTLQGGTHEQGLRGGLTRSLRAYGERISNR
                     KTSLITPDDLMASACAVLSVFIREPEFQGQTKEKLSSPDAQRLVENALRDHFDHFLSE
                     HPQEADGLLNSVIDAAEERLKRKQEREVSRKTATRKLRLPGKLADCSRDGQNGTEIFL
                     VEGDSAGGSAKQARDRATQAVLPLRGKILNVASAAAGKLQQNQELSDLVQALGCGVGA
                     KYRDEDLRYERVIIMTDADVDGAHIASLLLTFFFREMRGLIANGHLHLAMPPLYRLTQ
                     GAKSVYARDDADKAALIKSAFKASSKVDVSRFKGLGEMMPMQLKETTMKVGSRVLLKV
                     EIAESDASDTDNLVERLMGKKPELRFQFIQENAEFAKDVDV"
     gene            10870..12480
                     /locus_tag="VGM72_06330"
     CDS             10870..12480
                     /locus_tag="VGM72_06330"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY4264917.1"
                     /translation="MIRDRRPKWSFGAVNISIESLAGSNASTLGEVPRGPAVSTPSTI
                     SDVRFEDLGLGPQLLKAVADSGYTTPTPIQARGIPEVLKGKDIIGIAQTGTGKTASFT
                     LPMIEILARGRAKARMPRTLILEPTRELAAQVAESFERYGKYHKLSMALLIGGVSFDD
                     QDKKLDRGVDVLIATPGRLLDHFERGKLMMTGVQILVIDEADRMLDMGFIPDVERIAK
                     LTPFTRQTLFYSATMPPEIQRLTDQFLQAPVRIEVSRPATAASTIAQMAITVPGGDWS
                     KRETLRRLINENEVKNAIVFCNRKRDVDVVAKSLIKHGFSAAALHGDLDQSVRTRTLE
                     AFRSGTIRLLIASDVAARGLDIPAVSHIFNFDVPFQPDDYVHRIGRTGRAGRAGHAFM
                     LVGARDEKSVAAIEKLMGLTIPRRAMEGLPEEMPRPERTREARGRERTRGRERERVPH
                     GHRASYEPHVATAPAVPAPEAHLVAAQTPARDSASRHQAGKSEPRRKQAEPRSGGHDA
                     SQLPAFLLRPVKLPPKPVRKLADAENES"
     gene            12682..13659
                     /locus_tag="VGM72_06335"
     CDS             12682..13659
                     /locus_tag="VGM72_06335"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY4264918.1"
                     /translation="MPANFEVAYGLVAGDPPELKRAVETLLANGREFDASVMTELRQR
                     HFPTPKVENAIEELGEKIAAELDSVLQLLQTAGRDNSAYGKTLSKASGELGHAKIGEA
                     GLKSLIDGVVGATRAMEARSKTLENELQKSSAEMDDLRRNLESVRKESLTDQLTGIPN
                     RKAFDTELEESIAHSIETGEPLSLVMCDIDRFKIFNDTWGHQTGDQVLRLVANCLSEN
                     VKGRDTAARYGGEEFVVILPQTALIGAVHLANQIRTKVESKKLVKKSTGDILGVITIS
                     AGVTQFDPRESVEDFIRRADACLYAAKHAGRNCVISEKDNAARFESTAA"
     gene            complement(13666..14067)
                     /locus_tag="VGM72_06340"
     CDS             complement(13666..14067)
                     /locus_tag="VGM72_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020056172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEY4264919.1"
                     /translation="MTATNTNSTTRPRRERPKVIRLTDVAAARIRDIMEKSDGRYVGV
                     RVGVKNGGCAGMSYTMDYAESQTPLDEVIEDKGVRILIDPKAVMFLIGTEMDYKRDKL
                     AARFVFSNPNQTESCGCGESVTILPAKPETD"
     gene            complement(14076..14492)
                     /locus_tag="VGM72_06345"
     CDS             complement(14076..14492)
                     /locus_tag="VGM72_06345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HEY4264920.1"
                     /translation="MSEDTNIAVKSEQEVTEAVTPGASVNPGQASALSPEELEKFGNE
                     LIAAMKTVYDPEIPVDIYELGLIYKVDVADNKDVTVDMTLTAPGCPVAGEMPNMVRDA
                     LLTVPGVGEVTVNMTFDPPWTPERMSEEAKLELNMF"
     gene            complement(14489..14944)
                     /locus_tag="VGM72_06350"
     CDS             complement(14489..14944)
                     /locus_tag="VGM72_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEY4264921.1"
                     /translation="MDDNLRELYQEIILDHSRHPRHFGALESANHVAEGFNPLCGDRV
                     KVYLQVDANDRIVDVAFEGRGCAISVASASLMTEMLKGRDIGDAEKLMDGFVHLVKGD
                     AVDGLESDDREQLEVMAGVSAFPMRVKCATLAWHAMKAALDDEAVAKTE"
     gene            complement(14944..16191)
                     /locus_tag="VGM72_06355"
     CDS             complement(14944..16191)
                     /locus_tag="VGM72_06355"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEY4264922.1"
                     /translation="MNAEPQVRNATYDVAALRRDFSILSREVHGKPLVYLDNAASAQK
                     PRAVLDAMHSFVTSEYANVHRGVHYLSGAATERYEEARRRIQRFFNAAHEDEIIFTKG
                     GTEAINLASASYLAPRIESGDEIVLSVMEHHSNIVPWHFLRERHGACLKWVEVSDLGE
                     LDPADIAHAITPRTKLVAISHMSNVLGTKTDLREIVRIAHENGVPVLADGCQGAVHGI
                     VDVQALDVDFYAITGHKLYGPTGIGALYAKRAHLKTMRPYQGGGEMIREVTRDRVTYA
                     DAPMRFEAGTPPIVQTVGLAAALDYLDSIGRENIAAHETALARVAARRLKELNGVRII
                     GEAHDKGPIISFEAIGIHAHDLATILDREGVAVRGGHHCAQVLMERFNVSGTARASFG
                     LYNTMDEVDALVAAVGVARKVFG"
     gene            complement(16188..17495)
                     /gene="sufD"
                     /locus_tag="VGM72_06360"
     CDS             complement(16188..17495)
                     /gene="sufD"
                     /locus_tag="VGM72_06360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEY4264923.1"
                     /translation="MTAHPFKLDSQPNMFREDFSRHSAELPGAGLAWLDARRRAAMDA
                     FDRTGVPTRRVEAWKYTDLANALESDLVPASRFEGEIAFDDAFGARGGAQLLLADGFL
                     HSATGTDGMELVDLAALGSQTPAWVEEHLGRMAAGAAQPLGAASLGLMRGGVALRVHG
                     TRSLHLHFARSAGAQDSVSHGRVLIVVEDGASLRLTETHTGGGASESLVNLGIELVLK
                     ENAKLEHIRIQSETARTLHVTSLGASLARDAEYRALYAAMGARLSRMDVNLNLAAPGA
                     HATLHNVAVLSAGIADVTTVMDHAAPHTTSRQLFKSVVGGCGRSVNQGRVTVRKGANK
                     ADSHQLFKALLLSPRAEVDAKPELEIFADDVVCGHGTAIGALDADALFYLRARGIPEN
                     EAKGLLIRAFLADAIEDFGDETLRDALGKRLDDALDVVERVAP"
     gene            complement(17492..18235)
                     /gene="sufC"
                     /locus_tag="VGM72_06365"
     CDS             complement(17492..18235)
                     /gene="sufC"
                     /locus_tag="VGM72_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEY4264924.1"
                     /translation="MLEIDDLHVTVDGKEILKGITLSIPAGQVHAVMGPNGSGKSTLS
                     YTLAGREGYQVTRGRILYRGEDLTALAPEERAAKGVFLALQYPVEIPGVVTLTFLKTA
                     LNAQRRAQGLPEFDALRTLKLVREKAATLRIGEDMLRRPLNAGFSGGEKKRMEILQMA
                     LFEPKLAILDETDSGLDIDALKLVAEGVNMLRSPDRAMLVITHYQRLLSYIVPDRVHV
                     LAGGRIVKSGGADLALELEKSGYEGVEAA"
     gene            complement(18262..19719)
                     /gene="sufB"
                     /locus_tag="VGM72_06370"
     CDS             complement(18262..19719)
                     /gene="sufB"
                     /locus_tag="VGM72_06370"
                     /inference="COORDINATES: protein motif:HMM:NF008773.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEY4264925.1"
                     /translation="MAAVRETTERVADLAGEKYKYGFVTDIEMVRSERGLNEDIIRYI
                     SAKKIEPEWMLQWRLEAFERWKEMTEPNWARVHYPKIDYQNIYYYAAPKSQGDRPQNL
                     EDVDPKLLETYKKLGIPLSEQKLLAGVAVDAVFDSVSVATTFKAKLTEAGVIFCPISE
                     AIRDYPDLVKKYLGSVVPVTDNFFATLNSAVFSDGTFVYVPKGVRCPMELSTYFRINA
                     SDTGQFERTLIIADEGSYVSYLEGCTAPKRDQNQLHAAVVELVALDRAEIKYSTVQNW
                     YPGDEEGKGGIYNFVTKRGDCRGKGSKISWTQVETGSAITWKYPSCILRGDDSTGEFY
                     SIAITNNYQQADTGTKMIHLGKNTRSRIISKGIAAGRAQNTYRGLVSVHPRAENARNH
                     TQCDSLLIGGNCGAHTVPYIEARNPTAKLEHEATTSKISDDQLFYCRQRGIPEEEAVA
                     LIVNGFCRDVLQQLPMEFAVEAQKLVGISLEGSVG"
     gene            complement(19729..20910)
                     /locus_tag="VGM72_06375"
     CDS             complement(19729..20910)
                     /locus_tag="VGM72_06375"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEY4264926.1"
                     /translation="MIGRGMVYLDHNANSPLRPLARGAMDDALGTSGNASSIHGAGRA
                     ARARIETAREQVARLADAPSTSLVFTSGGSEANALALKGMVAGALADEDRITRLFVSA
                     TEHDSVRANAAALAETVPGLKVSDIPVTPDGTIDLGTLRLALMQGKGRVLVAVMAANN
                     ETGVLQDIGKIAKLVKAEGGEDALFHVDAVQAAGRTALSFNESDADTMTLSAHKLGGP
                     QGAGALILKEGVPFVPLIAGGGQELRRRAGTENVAAIAGFGAAAVEAMDFSIAAATLQ
                     RLRDRFERALKGLAPDAVIFGGNASRLPNTSNFAIPGLAAETALIALDLDGVAISSGA
                     ACSSGKVWPSHVLSAMGVDEALARGGLRVSFGWNSREDDVDAAIASLRNLVARRAALA
                     A"
     gene            complement(20911..21390)
                     /locus_tag="VGM72_06380"
     CDS             complement(20911..21390)
                     /locus_tag="VGM72_06380"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY4264927.1"
                     /translation="MKLSTRGRYAVMAMADLARYADERVDSGEPVKLCEIAGRQELSL
                     SYLEQLFAKLRRGGLVVAARGPGGGYRLAHPASQTRIADIIVAVDEPIKATRCKSESS
                     KGCLGRTGRCLTHDLWEELGRQIEVFLESVTLADVLERRVLGRARSLGAVDSPRAVA"
     gene            21657..22313
                     /locus_tag="VGM72_06385"
     CDS             21657..22313
                     /locus_tag="VGM72_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4264928.1"
                     /translation="MPDIILPGPAGRIEARYQRQKAPNAPMALILHSHPQYGGTMNNP
                     LVYDLYHRYHKLGCTVVRFNFRGVGRSQGSFDNGAGELSDAAAVLDWMSTLYPNPSYC
                     WVAGISFGAWIGMQLLMRRPEITGFVSIAPQANLYDFAFLAPCPASGLFVHGTKDQVV
                     PEADVQKLVDRLTAQKGIKITYTKIEGANHFFDNHQPEVVDVVEEYVKKRMASTDGGR
                     "
     gene            22405..24300
                     /locus_tag="VGM72_06390"
     CDS             22405..24300
                     /locus_tag="VGM72_06390"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4264929.1"
                     /translation="MRNLFASMVSAAAFVWIGAAIADAPADINRTNPDQANPDRDWDW
                     CNGTVDGGVPFDVQIAGCTARIDAASETGISLFAAYYNRGFAYYNKGEYDHALADYDS
                     AIAVKPDEANGFVGRGLAHFHKKQYEEAAADFTRAIQLTPNDADVYYQRATASAALTR
                     YDNAIADLNRAISLKRNFAEAFFNRGGVFYAQHAYGQAVADLNQFLRLRSDSPEGYKT
                     RGLALYAIGELNKAIDDFDTAIKLNPRDPEVFYARALAHSAKEQYELAIADFDSALRL
                     KADYVEAIRYRGLAHDRTGMTDAAMADFNRAIRTRPDYGGAFGNRGYEYMKRGQYDLA
                     LSDLNRAIQLDPDLAEAFHYRGYVLHNVQEYDAAIADYDHVLELQPENVNALFGRAFD
                     YYHNGNFDSAIADYDQVIRLNPEASALLNRGSAYFGKHDYDHAMADFGRAIAMNPNYA
                     EAFYDRATAAAAKGDLAQAIADFGRAIALKPDYIEAFYNRAGVYDQDSQYERALSDFD
                     EVLKLKPNDAEAFVNRCWTRAVMDAALDTALDDCNQAIQLNANKDFTFDARGFVKLRM
                     GLLKDAIADYQLALDHNPKLSSALFGRGVAELRSGDATRGQADIALAETVRPGTAKNF
                     ERYGVTP"
     gene            complement(24290..25396)
                     /locus_tag="VGM72_06395"
     CDS             complement(24290..25396)
                     /locus_tag="VGM72_06395"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEY4264930.1"
                     /translation="MADVLKVIGLMSGTSLDGIDAAILDTDGESIAVPGPALTRPYDP
                     ATRAMLHAALRLAGHVQVGASVPAEIVKAEQLLTAAHAEAVDALLRVADLKPAAIAYV
                     GFHGQTVLHRPSERRTWQIGDGEALARATGIPVVNAFRLADVAAGGQGAPFAPLYHLV
                     LAKNMQRPLVVVNIGGVANVTYVGSEGEVMAFDTGPGNATLDDWALKHTGEAVDRDGT
                     LARSGRADETVLAAMLKHPSFAQTPPKSLDRFDFSDTPVAGMAAADGAATLTAFTAAA
                     IARAREHLPDAPHAWIICGGGRHNPALMVELRARLQGTVMSAEDAGWRGDFLEAEAFA
                     YLAVRSVRGLPLSLPATTGVPYPMPGGHLHRPTA"
     gene            25438..26700
                     /gene="tyrS"
                     /locus_tag="VGM72_06400"
     CDS             25438..26700
                     /gene="tyrS"
                     /locus_tag="VGM72_06400"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEY4264931.1"
                     /translation="MAPAPKFASEFLRTFVERGAYYDCSAPEDLDALFQKERVTAYIG
                     FDCTASSMHIGNLVQLMTLRRLQQAGHRPILLMGGGTTKLGDPSGKDDVRKLLTPEQI
                     EANKNAMVAGIRHLLKFGDGPSDAILVDNSDWLDRLDYIPFLRDVGRHFSVNRMLTME
                     SVKIRLEREQALSFLEFNYMVTQAYDFVELYRRYGCRLQSCGSDQWGNVVSGIDLGRR
                     MESVQLFGLSTPLITTSSGAKMGKTAAGAVWLNADMLSPYEYWQFWRNTEDNDVGRFL
                     RFFTDMPAGEIARLEILEGAERNEAKKILATEATAIVHGRPAADAAADTARRTFEEGT
                     TAAGLPTFEIERARLAAGIPVATLASLAGLTSSSGEARRFIQGGGLRLNDVPVTDVKA
                     MAGLGDLAPDGVIKLSVGKKNHMLIKPV"
     gene            complement(26701..>28713)
                     /locus_tag="VGM72_06405"
     CDS             complement(26701..>28713)
                     /locus_tag="VGM72_06405"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HEY4264932.1"
                     /translation="PIRVDADFPAGVLDQDFLPDEALTLSFPFEAMTARYIAGMTPIT
                     GARGEARLTGDSFHVTVAAASVGPLALSGGDVIIPDLHNSGVITRIKAHSEGKMSDVL
                     RLIDEEPLGYPKRFGVNPANVNGQAAVDLDVALPLLKDVSLDQLRIGVKATASELAMP
                     IDNRKLEHGMVNFTIDTQSLSSQGTGVLQGVPVSFKWTEDFIAPGVTTRVDLTGRLDD
                     ASRARLGLSDPQWLTGPMPVTLALYGRHFSLKDAGLKADLSAAVVDIPMLNLAKHAGT
                     RANASAQLHFGDAGALSMSDLVVTGEGMNVRGGLALDSEGKIVNVALSDVRMGANDFA
                     LNLEPLLGGDGLAVRIQGKRLDATHFFADKKKQADASSPAEMDSELQRPLSLNTKVDR
                     LVFHDDVGFRDVTMAVSFAANEKLTGFNLDAIGTGKGKVTGRMDVVKGVRNLSLDTDD
                     AGAFIDTFMNFSSVRGGKLAAHIDFPSDAQNAANAKAPLPDYQGVITLSDVVITDQPF
                     FARFFSAGSLDGPLRLLQGQGIPLTTVSVPFNARAKLVTIREGRAAGPAIGATFGGVL
                     DRKAEKLDLTGTLVPVYGINNILGAVPVLGDILNSRKGEGVFGLTYAMKGNLNEPTVT
                     LNPLSVLTPGIFRRIFEFSPPKDALAQPPPQASAESAPQTPAAPPQ"
BASE COUNT         5985 a         8954 c         8270 g         5504 t
ORIGIN      
        1 gcgatctcga atgacgacat gctgtggtac ggcgattata cgcgcggcta cctcggcatg
       61 tacgatccca ggaccggcaa ggcgaaggaa tggaaatcac cggccgggcc ggactccgca
      121 ccctatggca tcacgacgac ccccggcgca atctggtaca gcgagtccgg ggcgaacccc
      181 aacacgctgg tgcgcttcga tcggaagtcg gagaaattcc agacctggaa aatcccgtcc
      241 ggcggcggca cggtgcgcaa catgatggcc acccgggacg gcaatcttgc gatcgccgag
      301 tccggcgaga acgtgatcgc gctggtcgag atcaaatagc cggtcgatcg cagctaggat
      361 ttcctgaaat tttcctttga agctataaaa gcaagcaggt cggcattgat gatttccgca
      421 tgcgtcgtgg gcatcccatg cgggaagcca ggataagtct tcaatgaacc gtgcttcaaa
      481 agcttgattg ttctcgcgcc cgaggcgggg aacggacaaa tttgatcgtc ctcgccatgc
      541 atgaccagaa ccggcacgtc gattgcttta aggtcgtcga agaactcagt ctccgagagg
      601 actttgacgc aatcatactg ggccttgatc gcgcccatca taccttggcg ccaccaattc
      661 tcgcgtatgc cttccgagat gaccgcgccc gcacggttaa agccgtagaa cggcatggtg
      721 atgtctttgt agaattgtga gcgatggttg gcggttccgt cgcgaagacc gtcgaaaacc
      781 tcttttggaa gtccttcggg attggtgtct gttttcatca gcgcgggtgg aatggcgctg
      841 atcaacaccg ccttcgccac gcggcccgtg ccgtacttgg cgacataacg caccacttct
      901 ccgccaccgg ttgaatggcc gatatgcaca gcgcctttaa tatcaagtgc tttcgtgagt
      961 tcggcgacat cggctgcata ggtgtccatg tcatggccgt tcgctgtctg cgtcgagcga
     1021 ccgtggccgc ggcgatcatg ggcaataacg cgatagccct tccccaggaa gaacatcatc
     1081 tgggcgtccc aatcgtcggc tgacagcggc caaccatggt gaaagaaaag cggcgtgccc
     1141 gatccccagt ctttatagaa aatctcggta tcatccttgg tggtcaacat cggcatatct
     1201 tgtttccttt tcagaatgtg cggacaagcc tgtcgtgcgc tggaaaacca tacgaagatc
     1261 ggacgatatt gtgcattggc ggaaatgaca ttgttcgggg caaaactgac aaatggcgat
     1321 ttgggatcca cccaccgaaa ttggcatcct cctctaccct gaagttgcat ccgcaacggt
     1381 tcatggactg acggatctct tcactgtcgc gacaacgctc gttcgcgaac atctggggcc
     1441 aaccgcaccg actctgcgta taagccattg gcaaccgggt tctacaaaca acgcgatcga
     1501 atgtgtcttc gacacgcacc ctcagttcgc tactagacct gtatcggtga tcgttcccgg
     1561 aagctggaaa ggccaaccgg cctcagaggt aaggcgccgc cttgtccagt ggctcattga
     1621 gcgacatgcc gccggtacca cgctctgctc tgtttgcgga ggggccttca ttcttgccga
     1681 aaccggctta ctcgccgggc gctccgtcac cacgcattgg tccttaaagg gggagttggc
     1741 ggatcgcttt ccagagattc aagtcgatga aagcaggctc acgatcgagg atggaaactt
     1801 tatcacggca ggcggagtgc tagcgtgggc cgatttgggt ttgaagcttg tcgacaggta
     1861 cctcggaccg acaatcatgc tgcggacctc acagtttatg ctcgcggacc ctcccggccg
     1921 cgagcagcgg tattacagcc atttttcgcc aaagctccat cacggcgact cgtccattct
     1981 gaaggtgcag cattggcttc aaacgcaagc gaccaaaaag ccatcggtat cggagatggc
     2041 gaaagcgggt ggtctagagc cgcgaacgct tcatcgacgc tttcagaaag ccacaggcct
     2101 caaaccgaac gagtacagcc aacaccttcg cgtgggaaag gcacgggaga tgttggaatt
     2161 cacaaaccgt acagtcgatc aggttgcctc cgccgttggt tatgaagatc cgagttcgtt
     2221 tcgcagagtg tttcaaagag tggtgggctt atcgcccggc gcctaccgcc gccggtttgc
     2281 tgccgcgaat gccgctcgca ccgcgacctg aatagccgct ttggctatca gcagacacat
     2341 ctcggccggc aatatcggtt ttcgtgccgc atcaccgcgc aagcaaactt agcaagaagt
     2401 cattccgcat cccttcagta ccgcgggcaa ttgttcgggg atgtcttccg cgataaggcc
     2461 ggggccatag cggtttgccg cgtcgccatg cagccatacg ccggcgcatg cggcctcgaa
     2521 cgcgcccatg ccttgtgcca tcagacccgc gatcatgccg gtcaggcaat cgcccgagcc
     2581 tgccgtggcc agccagggtg gcgcgttggc attgatcgcc gcaataccgt cgggcgcggc
     2641 aatgacggtg tcaggcccct tcagcaacac cacgcatccg gcgatgcgcg cggcttcacg
     2701 cgtcgcgtcc agacgcgacg gcgcgtttct gagcaggtct ggaaagatgc gcgagaattc
     2761 cccctcgtgc ggcgtcagga ccatgccggc gtcaagctgc cggaacaacg cgttcggatc
     2821 atcgccaaac gacgtgaggc cgtcagcatc tatcgcagcg gctgcgccac aggcgagcac
     2881 tgcggccacc agatcttgcg tgcgcggccc gactccgcag ccaggtccga tggcgacggc
     2941 gttgagccgc ttatccgcaa gcagggcgct taaacccttg gcgccctcga atggcttcac
     3001 catgatggcg gtgagctgcg cggcattgac gcctacggca tcgagcgggc ttgccacgct
     3061 gacaagcccg gcgccaatgc gcaacgcggc gcgcgccgcc agccgcgccg cgccggtggc
     3121 gtgcatgggg ccgctcacga caacggcgtg gccgcgatcg tatttgtggc cggcacgccg
     3181 gattgtggga aacgatgcgc gccacagctc cggcgtgttt tcgaaactcc ttggacggat
     3241 ggtttccaga acgccctccg ggatgccgat atcggcaagg atgatgtcac cgcacaggcc
     3301 tctgcctggg aacaacagat gcgccgcctt cctgcggaag aacgtgaccg tctgcgcggc
     3361 gtgaatgcaa acaccgccgg cgccatccaa tggtcgtccc agatcgccgt gaagcccgct
     3421 cggcacatcg accgcaagaa tgggaatgcc gctcgcattc gctcggcgca cgacgtccgc
     3481 cacgatgccc tgtaacgggc gcgcgagtcc ggcgccgaat agcgcatcga cgatcaacga
     3541 tgcgtcttct aagacggctt cgtcgaatgg cacaatcgga ccgccccagc ggttcgccat
     3601 cgccgccgca tcgcccttta gggatgccgg tggggacagg cacgcgaccc gcacctcgac
     3661 cccacgctcg cgcaattttg cagcggcgca gaatccatcc ccgccattgt tacccggacc
     3721 gcaaagcaca agcgccgcag cattgggatg atgcgccgtc accgcattgg cgacggcgtc
     3781 cccggcattc tccatcagcg tcagcgaggg aatgccgcct tcaacggaaa gcaggtcggc
     3841 ctctcccatt tgggcaatgg tgaggatttc gccgctcact tcgaatccct atcccgcaag
     3901 agcggagaaa taattgttca accgtttgcc cgcgtcttcc agaaccgcgc gttttttgca
     3961 gaacgcaaac cgcaccaggt cggcgggtcc gccttcgcta taaaataccg acagcggaat
     4021 gggtgtgatc ccggcctccc gcgttatgcg cttgcagaat tcgcgatcgg tttcgttggt
     4081 taatccacgg atcgaggcgg tgatgaagta agtgccttcg ctggagagca catcgaatcc
     4141 aaccgcctgc agcgtgccgg ctatcgaatc gcgtttaacc tgaagcgcgc gcgtattctc
     4201 cagtgcataa tccatttcgt gctccagccc gtgcgcgacg cccagttgca gcgccggcga
     4261 agtcgtgaag gtgttgaact ggtgggcttt ggaaatcacc tggatcaggc gggcgggacc
     4321 gcaaatccag ccgatcttcc aaccggtcag ggaaaacatc ttccctgccg aaccgacgcg
     4381 cacgcagcgt tcgcgcatac cgggaagcga gatcagcggc acgtgggcgc gtccgtcgaa
     4441 caccagatgc tcgtacacct cgtcacacac cgcataggca tcatagtgct tcaaaaattg
     4501 cgcgagcagc tccagctcgg cgcggtcgaa cacccgcccg atcgggttca agggcgaatt
     4561 gacgatgata agcttggtcc gctccgaaaa aacagcggcc agcgcgcctt cggtcaggcg
     4621 gaaatccggc gtggcgagcc gcagactcct cactcgtgcg cccatggcgg cgagcatcgg
     4681 aggataggaa tcgtagcaag gctcgatcag gaccgcctcg tcacccggcg cgatcagcgc
     4741 catgacgcaa tccgtgagcg cctcggtggc gcccgaggta atgagcactt cggtttcggg
     4801 atcgaacgcg aggtcgtaaa agcgttttgc atgaccggcg acagcttgcc gcagcagagg
     4861 caaaccttgc atgggcggat attggttggg tccatcgcgc agtgcccgcg ccgcggcttc
     4921 gcggatgccg gtcgggccgt cctcatcggg aaaaccctga cccagattga tggcgccatg
     4981 tgcgcccgcg agtgccgaca tcactgtgaa aatggtggtg ccgagccctt cgaacaccgg
     5041 gttcaagttg cgcgatgtca tcaaaaacct ctccgccggc ccgcaacttg gcaaagcgct
     5101 tcgattgggg caatgccgga ttttcggacg taattcaggc agattgcaca aaatataagc
     5161 aagccgctga gggcacgccc tgccttaacc gataaactgc tgaaaaactg cgcggaaaga
     5221 caattgcagg ggtggcacgc accctgctag ttccttctgt gcgagtgccg catctgacgg
     5281 gaaggtctcc atgaaaaaag tcgaagcgat cataaagccc ttcaagcttg acgaggtgaa
     5341 agaggcgctc cacgaggtcg gcgtccaggg gatcaccgtc acggaggcca agggattcgg
     5401 acggcaaaaa ggccataccg aactttaccg cggcgctgaa tatgtcgtcg atttcctgcc
     5461 caaggtgaaa atcgagatcg tactcatgcc cgaacaggtg gagccggcgg tggaagcgat
     5521 tttgaaggcc gcgcgcaccg ggcgaatcgg cgacggcaag attttcatac tcgatgtcga
     5581 ggaggccgtc cgtattagga ccggcgaaaa aggggcagac gcgatctgaa agcgcgcaag
     5641 ttttccgtcc agtttttcaa cgcttaaatc caaacaggaa cagaaccgat ggctgatgca
     5701 aaatccgtcc tcaaaatgat caaggacaaa gaagtcaaat tcgttgatct tcgtttcacc
     5761 gatccgcgcg gcaagtggca gcatgtcacg ttcgaccttt ccatgatcga cgaaagcctc
     5821 ttctcacaag gcacgatgtt cgacggttcc tccatcgccg gctggaaggc gatcaatgaa
     5881 tccgacatgg tcctgatgcc cgatcccgat tcggccgtaa tcgatccgtt cttcgcccag
     5941 acgactctgg ccatcaattg cgacattcat gaacctctga cgggccaacc ctataatcgt
     6001 tgcccgcgct cgatctccaa ggcagcggaa aaatatctcg cttccgctgg catcggcgac
     6061 accgcctatt ttggtccgga agccgagttt ttcattttcg acgacgtccg tttcgatgtc
     6121 ggcatgaaca agggttattt tttcctcgac gctctggagg gcgcctacaa caccggcacc
     6181 gaatatgaga gcggcaatct tggccaccgt ccgcccgtta aaggcggcta ctttccggta
     6241 ccgcccgtgg actccgcaca ggatatccgt tcggagatgc tcgccatcat gggcgacatg
     6301 ggcatccagc cggaaaaaca tcaccacgaa gtcgccaccg cgcagcacga actcggcttc
     6361 aagttcaata cgcttaccaa gtgcggcgac aacatgcaga tctacaaata tgtgatccat
     6421 caagttgcgg cttcctacgg caaatcggcg accttcatgc ccaagccgat ctatggcgac
     6481 aatggttcgg gcatgcatgt gcaccagtcg atctggaaag aaggcaagcc gctcttcgcc
     6541 ggatcgggct atgccgacct gtcggagctg tgcctttact acatcggcgg catcatcaag
     6601 catgccaagg cgctcaacgg cttcaccaac ccgctgacca attcctacaa gcggctgatc
     6661 cccggcttcg aggcgcccgt gctgctcgcc tattcggcgc gcaaccgctc ggcctcctgc
     6721 cgcattccat tcggggcagg tcccaaggcc aagcgcgtgg aagtgcgctt ccccgatccg
     6781 ggcgcaaatc cttatctcgc ctatgcgtcg atgctgatgg cgggcctcga tggtatcgag
     6841 aacaagatcc atcccggcga gccgatggac aaggatctct atgcgcttcc gcccgaagag
     6901 ttgaaggaca tcccgcaggt ttgcggctcg ctgcgcgaag cgctgtcgca tctcgatgcc
     6961 gaccgcgcct tcctgaaaaa gggcggagtc tttaccgacg atttcatcga ttcctacatc
     7021 gaactgaaaa tgggcgaggt ctatgccttc gagcacaccc cgcacccggt cgagttcaag
     7081 atgtattatt ctgtctgacg tctgcacaaa accgtaatcg aaagggcgcg atgcgaatcg
     7141 cgcccttttt tctattctgt acgttggcgt tacgttggcg ttttcaggcc gacaggcgac
     7201 gcttcgggcg ctcttgcacc atcgcggtca tgggcagcac cacatgcacg cgggtgccgc
     7261 caccttcttc ggattcgagc cacgcgcgac cgccatgaag ctcggcgagc cccctgacga
     7321 gcgcgagccc taggccggtg ccgctgttcc cgctgtcgta gcgattgtcc acccgcgaga
     7381 acggtttgaa gatcttgccg atcttgtcct tgggaatacc gggcccgtta tcggcaacgg
     7441 tcaggtcgaa atcgccggtg ttgttgcgtt tcgcggtcat ttcgatatgt ccgccgtcgt
     7501 gggtgaactt gaccgcgttc gaaacgagat tgatgacgat ctgcttcagt gcgcgttcgt
     7561 ccgcatacag gatcgccgca tcgtcggcga cgctgatgct gagggtctgc ccgcgttcgc
     7621 gggctctggc gctcacgaat ttcagggcgc cgtcgagcgt tcgccgggtt tcgatgacct
     7681 ggggttcgat ctccatgcgc ccggattcga tcttcgcgac gtcgagcaga tcattgataa
     7741 gcgaaagcag atgagcgccg gaattgtgaa tatcccccgc atattccttg tagcgcggat
     7801 tgcccaccgc gcccagacat tcacgcgcta tgatctcgga aaaaccgagg atcgcattca
     7861 acggcgtgcg caattcgtga ctcatattgg caagaaacgc cgtcttcgag gtattcgcgc
     7921 tttcggcttc cgcccgtttc ataagcgcct cgtcgcgtgt ctcttcgagt tctcgcgata
     7981 gatcttcgtg gctgaaccgg atcaacgctt cgtcgtccca gcggtcactg atatgcacac
     8041 tgtccaggaa tacctggatc gcgtaaagcg gcaccagcga ggcaagcacc atatccgtca
     8101 tctgctggga ggccacacat cgcaagaaca gcatggagcc catgggaaga tagctggcca
     8161 ggaagaatgg aatgtgattg gcccggatga caagaaagcg cccgacgatg gcgaggcaca
     8221 tcaattcgat gaaaacgtga ttggttgtat tgacctcatc ccagagcagc cagggcgcgc
     8281 agccccaagc cgccgaaaga ccggcgctca tccaaagcag gcgcgatcgc cagcgtttcg
     8341 acgcaaccgc atcgacggcg ctttcgcggc agcgggaaaa tgtacgcagc accagagtcg
     8401 cgacacctgc ggtcagggtg acgacaagga caagcgcaag cgcgttgagg gcggaaacct
     8461 ttcccaaaaa aggcatgggg tcgctgcaca tcagcgcaaa gaagcacacc cacagcggcg
     8521 aacccgcgat ggcgccataa atggcgttgg ccgcccgctc gacctgaacc ttcaggattc
     8581 gcggatcggg agcattgctc gcagttctgg cctgcatcat cgcaatatcc gaataatttt
     8641 ccgctgcttt tctttgcaat tgcggcttgt gccagacatt accggcccaa agttaagaat
     8701 tgctgagatg gattcgcagc aatccgctgc ttcatgggaa acaacccgca caggcaagag
     8761 cggttttcct ccaaacccag aatcgggcaa aaggagccaa tggcaaagat acgcgatctt
     8821 ttcgaggaac cggctgaacc ggccgcgctt tccagccggg gaccggcaga caaaagctac
     8881 accgccaagg acatcgaggt cctggaaggc cttgagccgg ttcggcgccg gcccggcatg
     8941 tatatcggcg gcaccgatga acgcgccctg caccatctgg cggccgagat catggacaac
     9001 gccatggacg aggccgttgc cggctatgcc aatcggatcg aaatcgagct gaagccggac
     9061 gatattctga tcgtacgcga caatgggcgg ggcattccca tcgatcccca tccgaaattc
     9121 aagaacaaat cggcccttga agtcatcatg acgacgctgc atgccggcgg aaagttcggg
     9181 ggcaaggtct atgatacctc cggagggctg cacggcgtcg gcgcctcggt tgtcaatgcg
     9241 ctgtccgaat ggattgaggt cgaggttgcg cgcgaccggc ggacctacaa acagcgtttc
     9301 gagcgtggcc acccggcggg caagctgaag gatctagggc ctgcccccaa tcgccgcggc
     9361 acaatcgtca gcttcaagcc ggacatcaag atctttggca cagcgaattt ttcgccacgc
     9421 gtcctttacc ggatggcacg gtcaaaggcc tatctcttcc gcggtgtcga aatccgctgg
     9481 aaatgcgatt caagcctgct caagccggaa tgggaaattc cggtcgagga tgtgcttaaa
     9541 tttccgggcg gattgctcga tttcctcaag ggcgaaattt ccgcctcaaa aaccgtcacg
     9601 cgtcccttct tcggtcagat tcaaaatcgc gacggaaaag gtgcggtgga atgggctatc
     9661 gcctggtcgc cgcagatcga ccctttcgtg cattcctatt gcaacacgat tccgacgctg
     9721 cagggcggca cgcatgaaca aggattgcgc ggcggactga cacgctctct gcgcgcctat
     9781 ggcgaacgca tatcgaaccg caagacctct ctgatcacgc cggatgattt gatggcgagc
     9841 gcttgcgcgg tgctttcggt cttcatccgc gagccggaat tccaggggca gacaaaagaa
     9901 aaactttcaa gccccgacgc ccaacgcctc gttgaaaacg ctttgcgcga tcatttcgac
     9961 cacttcctgt cggaacatcc gcaggaggcc gacggcctgc tcaattccgt gatcgacgcg
    10021 gccgaagagc gcctcaagcg caaacaggaa cgcgaggtga gccgcaagac cgcgacgcgc
    10081 aaattgcggc ttcccggaaa gctcgccgac tgctcccgcg acggccagaa cggcaccgag
    10141 attttcctgg tggaaggcga ttcggccggc ggctcggcaa aacaagcgcg cgatcgcgcg
    10201 acacaggccg tcctccctct gcgcgggaag atcctcaacg tggccagcgc cgccgccggc
    10261 aaattgcagc agaatcagga attgtctgat ctggtccagg cgctgggctg cggggtgggc
    10321 gccaaatacc gcgatgagga tttgcgctat gagcgcgtca tcatcatgac cgatgccgat
    10381 gtcgatggcg cgcacatcgc ctcattgctc cttaccttct tctttcgcga gatgcgggga
    10441 ctgatcgcca acggccatct tcatctcgcc atgccgccgc tctaccgcct gacccagggg
    10501 gccaaatcgg tttatgcgcg cgacgatgcc gacaaggccg cgctgatcaa aagcgcgttc
    10561 aaggccagca gcaaagtaga cgtatcgcgc ttcaagggcc tgggtgaaat gatgccgatg
    10621 cagctcaagg aaaccaccat gaaggtgggt agccgcgtac tgctgaaggt ggagatcgcg
    10681 gaaagcgacg cctccgacac cgacaatctg gtggaacggc tgatgggcaa gaagccggag
    10741 ctgcgattcc agttcattca ggaaaacgcc gaattcgcaa aggacgtgga cgtgtaatcg
    10801 cctgaatggc cggcgtgtgg gttgacaatt aagacattat ccctatgttc catcgcaact
    10861 tataaggcca tgattcgaga caggcgtccg aaatggtctt ttggagcagt aaacataagt
    10921 atcgaaagtc tggctggctc gaatgccagc acactcggcg aagtgcccag ggggcccgca
    10981 gtttcaacgc cttccaccat cagtgatgtc cgtttcgaag atctcgggct agggccccag
    11041 cttctgaaag cggttgccga ttccggctac acgaccccga cgccgattca ggcacgcggc
    11101 attccggagg ttctcaaggg caaggacatc atcggcattg cccagacagg tacggggaaa
    11161 accgcctcgt tcacgctgcc gatgatcgaa attctggccc gcggccgcgc caaggcgcgc
    11221 atgccgcgca cgctgatcct tgaaccgacg cgtgaattgg ccgcccaggt cgccgaaagc
    11281 ttcgagcgct atggcaaata tcacaagctt tccatggcgc tcctcatcgg cggcgtctcc
    11341 ttcgacgacc aggacaagaa actcgaccgc ggcgtggatg tgttgattgc aacgccgggc
    11401 cggctgctcg atcattttga gcgtggcaag ctgatgatga cgggcgtgca gatcctggtg
    11461 atcgacgaag ccgaccgcat gctcgatatg ggtttcatcc cggatgtcga gcgcatcgcc
    11521 aagctgacgc ctttcacgcg ccaaaccctg ttctattcgg cgaccatgcc gccggaaatc
    11581 cagcgtctga cagaccagtt cctccaggcc ccggtgcgca ttgaggtgtc acgtcccgcg
    11641 actgccgcct cgaccatcgc tcagatggcc atcaccgttc ccggcggcga ttggtcgaag
    11701 cgcgaaacgc tgcgccggct catcaacgaa aacgaggtca agaacgcgat cgtattctgc
    11761 aatcgcaaac gcgatgtgga cgtggtcgca aaatccctga tcaaacacgg cttctctgcg
    11821 gctgcgctcc atggcgacct cgatcagtcg gtgcgcacgc ggaccctgga agccttccgc
    11881 agcgggacga tacgcctgtt gatcgcgagc gacgtcgccg cccgcggctt ggatatcccg
    11941 gcggtcagtc acatcttcaa tttcgatgtt ccttttcagc ccgacgatta tgtacaccgc
    12001 atcggccgaa ccggccgcgc cggccgcgcc ggccacgctt tcatgctggt cggcgcccgt
    12061 gacgagaaat ctgtcgccgc aattgagaaa ctaatgggcc tgacaatccc acgccgcgcc
    12121 atggagggtt tgccggaaga aatgccccgc ccggagcgca cgcgcgaggc gcgcggacgc
    12181 gaacgcactc gcggacggga acgcgaacgc gtaccacacg gacaccgcgc ctcctatgaa
    12241 ccgcatgtgg ccacggcgcc tgcggttcct gcccccgaag cgcatctcgt agccgctcaa
    12301 acgccggctc gcgattcggc ctctcgccat caggccggca aatccgaacc gcgccggaag
    12361 caggccgagc cgcgttctgg aggtcatgat gccagtcagt tgcctgcgtt cttgctgaga
    12421 cctgtgaaac ttccgccaaa accggttcgc aaactcgccg acgctgaaaa cgaaagctga
    12481 agctttccca tcttttccat ctggatttgc cgaaagccgc gcgaaactgg cgcggcttta
    12541 ccatttgtta cggaccatcc cccaaaatac gtacgcatcg cgtcaggggg acggggcttg
    12601 cggaccgaat tggataaaga gcgaacggaa actctcgcac gcgaagcgtg tgcctggatg
    12661 gcgcggcgga acattccgcc gatgccggca aatttcgaag tcgcctatgg tcttgtcgcc
    12721 ggcgatccgc cggagttgaa gcgtgccgtg gagacactgc tcgccaatgg ccgcgaattc
    12781 gacgcgagcg tcatgacgga actccgccaa cgccatttcc ctacgccaaa ggttgaaaac
    12841 gccatcgagg aactcggcga aaagatcgcc gccgagctcg actccgttct gcaattgctg
    12901 cagacggccg gtcgcgacaa ttccgcctac ggaaaaacct tgtcaaaggc ttccggcgag
    12961 ttgggccatg ccaaaattgg cgaagcgggg ttgaagtcgc tgatagatgg tgtcgtcggc
    13021 gccacgcgcg cgatggaagc gcgctccaag acgttggaaa acgaactcca gaagtcctcg
    13081 gccgagatgg acgatctgcg ccgtaatctg gagtccgtgc gcaaggaaag tcttaccgat
    13141 cagctcaccg ggattcccaa tcgcaaagct ttcgataccg agcttgaaga aagcatcgca
    13201 cattcgatcg aaaccggcga accgctgtcc ctggtgatgt gcgatatcga tcgcttcaag
    13261 atattcaacg acacatgggg ccaccagacc ggcgaccagg ttctcaggct ggtggcgaat
    13321 tgcctctccg aaaacgtgaa gggccgtgac acggccgcgc gttacggcgg agaggaattc
    13381 gtggtgatcc tgccgcagac cgccttgata ggcgcggtcc atctggccaa tcagatccgc
    13441 accaaagtag aatccaagaa gctggtgaag aagtccaccg gagacattct tggcgtcatc
    13501 acgatttccg ccggggtcac gcaattcgac cccagggaaa gcgtggaaga ctttatccgc
    13561 cgggcggatg cctgccttta tgcggccaaa catgccggac gcaattgcgt gatcagcgaa
    13621 aaagacaacg ccgcccgatt cgaatccacc gccgcctaag aggtttcaat cggtctcggg
    13681 ctttgccggc agaatcgtga ccgattcgcc gcaaccgcag gattcagtct ggttcggatt
    13741 gctgaacacg aaacgcgcgg caagcttgtc acgcttgtag tccatctcgg tcccgatcag
    13801 gaacatcacg gccttgggat cgatcaggat tcgcacgccc ttatcctcga tcacttcatc
    13861 gagcggcgtc tgtgattcgg catagtccat cgtgtaactc atgccggcac agccgccatt
    13921 ctttacgcca acgcgcacgc cgacatagcg gccgtcggat ttttccatga tgtcgcggat
    13981 gcgcgcggcg gcgacatcgg taagccggat caccttgggg cgctcgcgcc ggggtcgcgt
    14041 ggttgaattt gtgtttgttg cggtcatgat ctcgctcaaa acatgttcaa ttcgagtttg
    14101 gcttcctccg acatacgctc cggcgtccag ggcggatcga acgtcatatt caccgtaacc
    14161 tcccccacgc ccggcacggt gagaagcgca tcgcgcacca tgttcggcat ctcaccagcc
    14221 accggacagc cgggtgctgt cagcgtcatg tcgacggtga catccttatt gtcggccaca
    14281 tccaccttgt agataagacc gagttcataa atgtcgaccg gtatctccgg atcatacacg
    14341 gtcttcatgg cagcaatcag ctcattgccg aatttctcca gctcctcagg cgacaaagct
    14401 gaggcttgac ccggatttac ggacgcgccg ggggtaaccg cttccgttac ttcctgctcg
    14461 gatttcaccg cgatgttggt atcttcgctc attcggtctt cgccacggcc tcatcgtcga
    14521 gcgctgcctt catcgcgtgc catgccagcg tcgcgcattt cacccgcatc gggaacgcgg
    14581 agacaccggc catcacttcg agctgttcgc ggtcgtcgct ttccaatccg tccacggcgt
    14641 cgcccttcac cagatggacg aagccatcca tgagcttctc ggcatcgccg atgtctcgtc
    14701 ccttcagcat ctcggtcatc agcgaggcgg aagcgaccga tatggcgcag ccccgcccct
    14761 cgaacgcgac atcgacgata cgatcgttcg catccacctg aagatagacc ttcacccggt
    14821 caccgcagag cggattgaaa ccttccgcga catgattggc cgattcgagc gctccgaaat
    14881 ggcgcggatg gcgcgaatga tccaggatga tctcctgata aagctccctg agattgtcgt
    14941 ccatcagccg aacacctttc gcgcgacgcc gaccgcggca accagcgcat ccacttcgtc
    15001 catcgtgttg taaagcccga acgaggcgcg cgccgtcccg gacacattga agcgctccat
    15061 caggacttgc gcgcaatgat ggccgccgcg taccgccacg ccctcgcgat caaggatggt
    15121 ggccagatca tgcgcatgga tgccgatcgc ctcgaacgag atgatgggcc ctttgtcgtg
    15181 cgcctcaccg atgatcctca caccgttcaa ttccttcaac cggcgcgcgg cgactcgggc
    15241 aagcgccgtc tcatgggccg caatattctc gcggccgatg ctgtcgagat agtcgagcgc
    15301 agcggcaaga ccgaccgttt gcacgatagg cggcgttccg gcttcaaaac gcattggcgc
    15361 gtcggcataa gtcacgcggt cgcgcgtcac ctcgcggatc atctcaccgc cgccctgata
    15421 ggggcgcatc gtcttcagat gcgcgcgctt cgcatataga gcgccgatac ccgtcggacc
    15481 gtaaagcttg tgccccgtaa tggcgtagaa atcgacatcc agcgcctgca catcaacgat
    15541 gccgtgcacc gcgccctggc aaccatcggc cagcaccggc acgccgtttt cgtgtgcaat
    15601 acgcacgatc tcacgcaaat ccgtcttggt acccagcaca ttcgacatat gcgaaatcgc
    15661 caccaatttt gttcggggcg tgatcgcgtg cgcaatgtcg gccgggtcca actcgcccag
    15721 atcggaaacc tcaacccatt tcaggcaagc accgtggcgc tcgcgcagga aatgccaggg
    15781 cacgatattg gaatgatgct ccatcacgct gagcacgatc tcgtcgcccg attcgatgcg
    15841 gggcgccaga taggaagccg acgccagatt gatggcttcc gtgccaccct tggtgaagat
    15901 gatctcgtct tcatgcgcgg cattgaagaa acgctggatg cgccggcggg cctcctcgta
    15961 acgctcggtc gccgcgcccg aaagataatg cacgccgcga tgaacattcg catattcgct
    16021 cgtcacgaat gaatgcatcg cgtcgagcac agcccggggc ttttgcgccg acgcggcatt
    16081 gtcgagatag accagcggct tgccatgcac ttcgcgcgac aggatggaaa aatcgcgccg
    16141 cagcgccgcc acatcgtaag tcgcgtttct cacttgcggt tccgcgttca tggggcgacc
    16201 cgttccacga catcgagcgc atcgtcgagc cgttttccca acgcatcgcg cagggtttcg
    16261 tcgccgaaat cttcgatggc gtccgcaaga aatgcgcgga tcaacaaacc cttcgcttcg
    16321 ttttccggaa tcccgcgcgc acgcaaatag aacagcgcgt cggcatcgag cgcgcctatg
    16381 gccgtaccgt gcccgcaaac gacatcgtcg gcgaaaatct ccaattccgg ctttgcgtcg
    16441 acttcggcgc ggggagaaag aagcagcgct ttgaaaagct gatgcgaatc cgctttgttt
    16501 gcgcctttgc ggacagtgac ccgtccctga ttgacggaac ggccgcatcc accgaccacg
    16561 cttttgaaaa gctgccggct ggtcgtatgg ggtgccgcat ggtccatcac cgttgtgaca
    16621 tcggcaatgc cggcgctgag cacggccaca ttgtgcagcg tcgcgtgagc gcccggtgcg
    16681 gcaaggttca aattgacgtc cattcgcgaa agccgcgcac ccatcgcggc gtacagcgcg
    16741 cgatattcgg catctcgcgc aagcgaagcg cccaggctcg ttacatgcag cgttctggcg
    16801 gtctcggact ggatgcgaat atgctcaagt ttggcgttct ctttcagcac caactcgatc
    16861 ccgagattga cgaggctctc agacgcgccc ccgcctgtgt gagtctcggt caaacgaagg
    16921 gacgcgccat cctccaccac gatcagaacg cgcccatggc tcacgctgtc ctgcgcgccc
    16981 gccgaacgtg caaagtgaag atgcagcgag cgcgtgccat gcacacgcag cgcgacgccg
    17041 ccccgcatca aaccaagcga tgccgcaccc agcggctgcg ccgccccggc cgccatgcgg
    17101 cccaaatgct cctcaaccca agcgggcgtt tgggaaccga gcgccgcaag atcgacaagc
    17161 tccattccgt ccgtgccggt cgcgctgtga aggaatccat cggccagcag aagctgcgcg
    17221 ccaccgcgcg cgccaaacgc atcgtcgaac gcgatctcac cctcaaagcg gctcgcaggc
    17281 accaaatcgg attcgagcgc attggcgagg tcggtatatt tccaggcctc cacgcgccgc
    17341 gtcggcacgc ctgtcctatc gaaagcatcc atcgcggcgc gccggcgtgc atcgagccat
    17401 gcgagccccg cgccgggaag ctccgcgctg tgccgcgaaa aatcctcccg gaacatgttc
    17461 ggttgagaat cgagcttgaa cggatgggcg gtcatgcggc ctccacgccc tcatagccgc
    17521 tcttctccag ctcgagcgcg agatcggcgc cccctgattt gacgatgcgg ccaccggcca
    17581 gcacatgcac acggtccggc acgatgtagg aaagcaggcg ctgataatgt gtgatcacca
    17641 gcatcgcccg atccggcgag cgcagcatat tcactccttc ggccacgagc ttcagcgcat
    17701 cgatatcgag accggaatcg gtctcgtcaa ggatcgcgag cttcggctcg aatagcgcca
    17761 tctggagaat ctccatgcgc ttcttttcgc cgccggaaaa accggcattc agcggacggc
    17821 gcaacatgtc ctcgccaatc ctcagcgttg ccgccttctc gcgcaccaat ttgagcgtac
    17881 gcaaggcgtc aaattccggc agcccctgcg cgcgacgctg cgcgttcagc gcggtcttca
    17941 ggaacgtcag cgtcacgaca cccggaatct ccaccggata ttgcagcgcc aggaagacac
    18001 ctttcgcggc gcgttcctcg ggcgcgagtg cggtcaaatc ctcgccacgg taaagaatgc
    18061 ggccgcgcgt gacctggtag ccctcgcggc cggccagcgt gtaggacagc gtggattttc
    18121 ccgaaccgtt cgggcccatc accgcgtgca cttgacctgc gggaatggac agcgtgatcc
    18181 ctttcagaat ctccttgccg tctaccgtta cgtgcaagtc gtcaatttcg agcattccaa
    18241 tatcctgaac tttccgaagg atcatccaac gctgccttcc aggctgatgc ccaccaattt
    18301 ctgcgcttcg acggcgaact ccatcggcaa ttgctgcagc acgtcgcggc agaagccgtt
    18361 gacgatcagg gcaaccgcct cctcctctgg gatgccgcgc tgccggcaat agaaaagctg
    18421 gtcgtcggat attttcgacg tggtcgcttc gtgctcgagt ttggccgtcg ggttacgggc
    18481 ttcgatatag ggcacggtat gcgcgccgca attcccgccg atcagcagcg aatcgcattg
    18541 cgtatggttg cgcgcattct cggctctggg atgcacgctg acgagaccgc gataggtgtt
    18601 ctgggcgcgg cccgccgcga tgcccttcga gatgatgcgc gaacgcgtgt tcttgcccag
    18661 atggatcatc ttggtgccgg tatcggcctg ctgataattg ttggtaatgg caatagaata
    18721 gaattcgccg gtcgaatcgt cgccgcgcag gatgcagctc ggatatttcc aggtgatggc
    18781 cgatccggtc tccacctggg tccacgaaat cttggagcct ttgccgcggc aatcaccacg
    18841 ttttgtgacg aaattgtaga tgccaccttt gccttcctca tctcccggat accaattctg
    18901 caccgtggaa tatttgatct cggctcggtc cagcgccacc aattcgacga ccgctgcgtg
    18961 caattggttc tggtcgcgtt tgggcgcggt gcagccttcg agatagctca cataggagcc
    19021 ttcatcggcg atgatcaggg tgcgttcgaa ctggccggtg tccgaggcgt tgatgcggaa
    19081 ataggtggac aattccatcg gacaccgcac gcccttcggc acatagacaa aagtgccatc
    19141 cgaaaacacc gccgagttca gggtcgcgaa aaaattgtcg gtcaccggca caaccgagcc
    19201 gagatatttc ttcaccagat cgggatagtc gcgaattgcc tcggaaatcg gacagaagat
    19261 cacaccggct tcggtgagct tggccttgaa cgtggtcgcg accgacaccg aatcgaacac
    19321 cgcatccacc gccacaccgg cgagcagttt ctgctccgac agcggaattc ctagcttttt
    19381 atacgtttcg agaagcttcg gatcgacgtc ttccagattc tgcggccggt cgccctgcga
    19441 ttttggcgcg gcgtaataat agatattctg gtagtcgatc ttcggataat gcacgcgcgc
    19501 ccaattgggc tcggtcattt ccttccagcg ctcaaatgct tccagccgcc attgcagcat
    19561 ccattccggc tcgatcttct ttgccgagat atagcggatg atgtcttcgt tcagcccgcg
    19621 ctcggaacgc accatttcga tatcggtaac gaagccatat ttgtacttct cgccggccag
    19681 atcggcgacg cgttcggtgg tttcgcgaac agctgccatt tttctctcct aagccgccag
    19741 ggccgcacgg cgcgcaacga gattgcgcag gctcgcgatt gcggcatcca catcgtcctc
    19801 acggctattc caaccgaagc tcacgcgaag cccgccgcgc gcgagcgcct cgtccacacc
    19861 catggcgctg agcacatgag agggccacac cttgcccgac gagcaggccg cacccgacga
    19921 tatcgcaacc ccatcgagat cgagagcgat cagcgcggtt tcggccgcga gccctggaat
    19981 cgcgaaattg gacgtgttgg gtagacgcga cgcatttccg ccgaaaatca cggcgtcggg
    20041 tgccaaaccc ttcagcgccc gttcaaaccg atcgcgcagc cgctgtagcg tcgcggccgc
    20101 aatggagaaa tccatcgcct caaccgctgc ggcgccaaaa ccggcaatgg cggccacatt
    20161 ctccgtgcct gcgcgccgcc tcaactcctg gccgccgccg gcgatcagcg gaacgaacgg
    20221 cacaccctcc ttcagaatga gcgcacccgc gccttgcggc ccgcccagct tatgcgcgga
    20281 cagcgtcatg gtatcggcat ccgattcgtt gaaggagaga gcggtccgtc ccgcggcctg
    20341 caccgcatcg acgtggaaaa gcgcatcctc cccgccttcg gccttcacca acttggcgat
    20401 ttttccgatg tcctgcagca cgccggtttc attattcgcc gccatgaccg cgaccagcac
    20461 acgccccttt ccctgcatca gggcaaggcg cagcgtaccc aagtcgatgg tgccgtcggg
    20521 cgtcacagga atgtcgctga ccttcagtcc cggaaccgtt tcggcgagtg cggctgcatt
    20581 ggcgcgcacg ctgtcatgct cggtggcaga gacgaagagc cgggtgatgc ggtcttcgtc
    20641 cgcgagcgcg cctgcaacca tgcccttgag agcgagcgca ttggcttcgc tgccgccgct
    20701 agtgaatacc aatgacgtcg agggcgcgtc cgccaaacgc gcgacctgtt cgcgcgcggt
    20761 ctcgatgcgc gcgcgcgccg cacggccggc cccatgaatc gaggacgcgt tgccggaagt
    20821 gccaagtgca tcgtccatcg cgcctcgcgc gagcgggcgc aaaggcgaat tggcattgtg
    20881 gtcgagatac accatgccgc gaccgatcat tcaggctacc gcacgcggcg aatccaccgc
    20941 acccaggctc cgcgcgcggc ccagcacgcg gcgctccagc acgtcggcca aggtcaccga
    21001 ttccaggaag acctcgatct ggcggcccaa ctcctcccac agatcgtggg taaggcagcg
    21061 gccggtacgt ccgaggcatc ccttcgaact ctcggattta caacgcgtcg ccttaatggg
    21121 ttcatcgacg gcgacaatga tatcggcgat gcgcgtctgc gatgccggat gcgccaggcg
    21181 ataccccccg cccggtcctc gcgcggcaac cacgaggccg ccccggcgca atttcgcgaa
    21241 taattgctcg agataggaga gcgacagttc ctgcctgccg gcgatctcgc aaagcttgac
    21301 cggctcgccg ctgtccaccc tctcgtccgc ataacgggcg agatcggcca tggccatcac
    21361 ggcataacgc ccgcgcgtgc tcaatttcac tgacatctcc ttgcgccgca caaatgacga
    21421 tatgggcgaa gcatcaaaca cttgccattt gaacccgccg atgtagtaag aagagcgcgc
    21481 cacccgcgac atcggcggtt ccaggcggat atagaattcc tgagtagaat ggtcaagaat
    21541 tctaggactg caacccgcat tttttgatca aaaccctgac atgccaaccc tattttccgc
    21601 accccggatc tctaaatacc tgacaaaatt cccgaatatc cggggggaga ccacttatgc
    21661 ctgacatcat actgcccggg ccggcggggc gaatcgaagc ccgctaccaa cggcaaaagg
    21721 cgccgaacgc gccaatggcg ctgatcctgc attcgcatcc gcaatatggc gggacgatga
    21781 acaacccgct cgtctacgat ctctatcatc ggtaccataa gcttggctgt acggtggtgc
    21841 ggttcaactt tcgtggcgtc ggccgcagcc agggctcctt cgataacggc gcgggcgaac
    21901 tgtcggatgc ggccgccgtg ctcgattgga tgagcacgct ttaccccaac ccgtcttatt
    21961 gctgggtggc cggaatatcc ttcggcgcct ggatcggcat gcaactgctg atgcgccggc
    22021 cggaaatcac cggcttcgtc tccatcgcgc cacaggcgaa tctctacgat tttgccttcc
    22081 ttgcaccctg tccggcctcg ggattgttcg tacacggaac gaaagatcag gtggtgccgg
    22141 aagccgacgt ccagaaactg gtggatcgtc tcaccgcgca gaagggcatc aagatcacct
    22201 acaccaagat cgaaggcgcc aaccatttct tcgacaatca tcagcccgaa gtcgtggacg
    22261 tggttgaaga gtatgtgaag aagcgcatgg cctcgacgga cggggggcgg tagcctcgcg
    22321 tcttcgcgcg tgcctgcatt gtgcttgtca cctcctacgc caaatcctaa actcgccaaa
    22381 agatgatgcg caaaaggcgc gaagatgcga aacctgtttg catcgatggt atcggcggcc
    22441 gcgttcgtct ggattggcgc ggccatcgcc gatgcgcccg cagacatcaa tcgcaccaat
    22501 ccagatcagg ccaatccaga tagagactgg gattggtgca atggcacggt tgacggcggc
    22561 gtgccattcg acgtgcaaat cgcgggctgc acggcacgca tcgatgcggc atcggaaacc
    22621 ggcatcagcc ttttcgccgc ttattacaat cggggcttcg cttattacaa taagggcgaa
    22681 tatgatcatg ctctcgccga ttatgattcc gcgatagcgg tcaagcctga cgaggcgaat
    22741 ggcttcgttg gccgcggcct cgcccatttc cacaagaagc aatatgaaga ggccgcagcc
    22801 gatttcaccc gggcaattca gctcacgccg aacgatgcgg acgtatacta ccagcgggca
    22861 acggcaagcg ccgcgctgac gcgatacgac aacgccatcg ccgatctgaa ccgcgcgatt
    22921 tcgctcaagc gcaatttcgc ggaagcgttt ttcaaccgcg gtggcgtgtt ctatgcgcag
    22981 catgcctatg ggcaggccgt cgcggacttg aatcagtttc tccggctcag atccgattcg
    23041 cccgaaggat acaaaacgcg gggcctggcc ttgtacgcga taggcgaact gaacaaggcg
    23101 attgacgatt tcgacactgc catcaaactc aacccgcgcg acccggaagt attttatgca
    23161 cgcgcattgg cgcacagcgc caaggaacaa tatgaattgg caatcgccga tttcgattcg
    23221 gcattgcgct tgaaagccga ttacgtagaa gccatccgct atcgcggtct tgcacatgat
    23281 cgcaccggca tgacggatgc ggccatggcc gatttcaacc gcgccatcag gacgcgtccc
    23341 gattatggcg gcgcgttcgg caatcgcggt tacgagtaca tgaagcgcgg acaatacgat
    23401 ctcgcgctct ccgacctgaa ccgggcgatc cagctcgacc ccgatctggc ggaagccttc
    23461 cactaccgcg gttatgtgct gcacaatgtc caggaatacg acgcggccat cgcggactac
    23521 gatcatgtgc tggaattgca gcccgaaaac gtcaacgcac tgtttgggcg cgcattcgat
    23581 tattatcata acggcaattt cgactccgcg atcgccgatt acgatcaggt gatccggctc
    23641 aatccggaag caagcgcatt gctcaatcgc ggtagcgcct atttcggcaa gcacgactac
    23701 gatcacgcga tggcggattt cggccgcgca attgcgatga atccgaatta tgcggaagca
    23761 ttctatgatc gcgccacggc cgcagccgcc aagggcgacc tggcgcaggc gattgcggat
    23821 ttcggcagag cgattgcgct caaacccgat tacatcgagg ccttttacaa tcgtgccgga
    23881 gtctacgacc aagacagtca atatgagcgc gcgttatcgg atttcgacga ggttctgaag
    23941 ttgaagccga atgacgccga ggccttcgtc aatcgctgct ggacccgcgc ggtcatggac
    24001 gcggcgctcg ataccgcact cgatgattgc aaccaggcaa ttcagctgaa cgccaacaag
    24061 gatttcacct ttgacgcacg cggcttcgtc aagctgcgca tgggtctgtt gaaagacgcg
    24121 atcgccgatt atcagctcgc gctggatcat aaccccaagc tttcgagcgc actcttcggg
    24181 cgcggcgtcg ccgagcttcg ctctggagat gccacgcgcg gacaggccga catcgcgctt
    24241 gcggaaacgg tgagacccgg gacagcgaaa aatttcgagc gttacggcgt cacgccgtag
    24301 gacgatgaag gtggccgccc ggcatcggat agggcacgcc ggtggtggcg ggaaggctga
    24361 gcggcaatcc ccgtacgctg cgcaccgcga gataggcgaa ggcttcggcc tccagaaaat
    24421 caccccgcca gcccgcatct tcggcactca tgaccgtgcc ctgcagccgc gcccgcaatt
    24481 caaccatcag cgcagggtta tgccggccgc cgccgcaaat gatccacgca tgaggagcat
    24541 ccggcagatg ctcgcgggca cgcgcgattg cagcggctgt gaacgcggtc agggtggccg
    24601 cgccatcggc cgccgccatg cccgcgacag gggtatccga gaaatcgaaa cggtcgagcg
    24661 atttcggcgg cgtctgcgcg aagctcggat gtttcagcat ggcggcaagg acggtttcat
    24721 cggcacggcc gcttcgcgcc agcgttccgt cgcgatcgac ggcttcaccc gtatgcttca
    24781 aggcccagtc gtcaagcgtc gcattgcccg gccccgtgtc gaacgccatc acctcgccct
    24841 cggaacccac ataggtgaca ttcgcaaccc cgccaatatt caccaccacc aatggccgct
    24901 gcatgttttt cgctaggacc agatggtaga gcggcgcaaa aggtgcgcct tgcccgccgg
    24961 cggcgacatc ggcaagccga aacgcattca cgacgggaat gcccgtcgcc cgtgcgagcg
    25021 cctcgccatc gccgatctgc caggtacgcc gctcggacgg acgatgcagc acggtctggc
    25081 cgtgaaaccc gacataggcg atggctgcag gcttcaggtc cgcgacgcgc aaaagagcgt
    25141 caaccgcttc ggcatgggcg gcggtcagga gttgctcggc cttgacgatc tccgcaggca
    25201 cggacgcccc aacctggacg tggcctgcaa gcctcaacgc cgcgtgcagc atcgcgcgcg
    25261 tggcggggtc atagggccgc gtaagcgccg gaccgggcac ggcgatcgac tcgccgtccg
    25321 tatcgagaat cgccgcgtcg atgccatcga gcgaggtccc gctcatcagc cctatcactt
    25381 taagaacgtc cgccattcgt ggtacacacc ctgccctttt cgcaaccagg ttatatcatg
    25441 gcgcctgcgc cgaaatttgc ttccgaattc ctccgcacct tcgtggaacg tggcgcctat
    25501 tacgattgtt cggcgcccga ggatctggac gcgttgttcc aaaaggagcg cgtgacggcc
    25561 tatatcggct ttgattgcac cgcgtccagc atgcacatcg gcaatctggt ccaactcatg
    25621 acgctgcgcc gcctgcagca ggcgggccat cgcccgatcc tcctgatggg cggcggcacg
    25681 accaagctcg gcgatccttc cggcaaagac gatgtgcgca agctgctgac gcccgagcag
    25741 atcgaagcca acaagaacgc catggtcgcc ggtatccggc acctcctgaa attcggcgac
    25801 ggcccgtcgg acgcgatcct ggtcgacaat tccgattggc tcgacaggct cgactacatc
    25861 ccgttcttgc gcgacgtcgg ccggcatttt tccgtcaatc gcatgctgac aatggagagc
    25921 gtgaaaatcc ggctcgaacg cgagcaggcg ctttccttcc tcgaattcaa ttacatggtg
    25981 acgcaggcct atgatttcgt ggagctgtat cggcgctatg gctgccggct ccaatcctgc
    26041 ggctccgacc aatggggcaa cgtcgtctcc ggcatcgatc tggggcggcg catggaaagc
    26101 gtgcaactct tcggcctctc cacacccctc atcaccacat cgtccggcgc gaagatgggc
    26161 aagacggccg ccggcgcggt ctggctgaac gccgatatgc tgagcccata cgagtattgg
    26221 caattctggc gcaacaccga ggacaatgac gtcggccgat ttttgcgttt tttcaccgat
    26281 atgccggccg gcgagattgc acggctcgag attctggaag gcgccgaacg gaatgaggcc
    26341 aagaaaattc tggcgacgga ggcgaccgct atcgtgcatg gccggcctgc cgcggacgct
    26401 gccgccgaca cggcgcgccg gacctttgag gaaggcacaa ccgccgctgg cctccccacc
    26461 tttgaaatcg aacgcgcacg gttggctgct gggattcccg tggcaacact cgccagtctt
    26521 gccgggctca cgagctcgtc cggcgaagcc cggcgtttca ttcagggcgg cggattgcgc
    26581 ctaaacgacg tcccggtgac agacgtcaaa gccatggcag gtcttggcga tttggcgccg
    26641 gacggcgtga tcaaactctc cgtcggcaaa aagaaccaca tgctcatcaa gccggtctga
    26701 ttattgcggc ggggcggcgg gagtttgcgg cgcggactcg gcggatgcct gcggtggagg
    26761 ttgcgccagc gcgtccttcg gtggactgaa ttcgaaaatc cggcgaaaaa tccccggcgt
    26821 cagaacagac agaggattaa gcgtcaccgt cggctcgttg agattgcctt tcatcgcata
    26881 ggtgagaccg aacacgccct cacccttgcg cgaattcaga atatcgccca ggaccggaac
    26941 cgcaccgagg atattattga tgccgtaaac cggaacgagc gtgccggtca gatccagttt
    27001 ttcggccttg cggtcaagca cacctccgaa tgtcgcgccg atcgcaggtc ctgccgcacg
    27061 gccttcacgg atggtcacga gtttggcgcg cgcgttgaac ggcacactga cggtggtgag
    27121 gggtatgccc tgcccctgca acaagcgaag gggcccatcc aggctgccgg ccgagaagaa
    27181 tcgtgcaaaa aacggctggt ccgtgatgac cacatcggag agtgtgatga cgccctgata
    27241 atcagggagc ggtgccttcg cattggccgc gttctgtgcg tctgacggaa aatcgatgtg
    27301 cgcagcgagc tttccgccgc gcacgctcga aaaattcatg aacgtgtcaa tgaaggcgcc
    27361 ggcgtcatcg gtatccaagg acaggttgcg cacgcccttg acgacatcca tgcggccggt
    27421 gacttttcct tttcccgtcc ctatcgcatc aagattgaaa ccggtcagtt tctcgttggc
    27481 cgcgaaagaa accgccatgg tcacatcgcg aaaacccaca tcgtcatgaa aaaccagcct
    27541 gtcgaccttt gtgttcagcg ataacggacg ctggagttcc gaatccattt ccgcgggcga
    27601 ggaggcatcg gcctgctttt tcttgtcggc aaagaaatgc gtggcatcca gccgcttgcc
    27661 ctgtatgcgc acagcgagcc cgtcaccgcc aaggagcggc tccaaattca atgcgaaatc
    27721 gttcgcgccc atgcgcacgt cggatagcgc cacattgacg attttgcctt cgctatccag
    27781 cgccaagcca ccccggacat tcattccctc tcccgtcacc accaaatcgc tcatcgagag
    27841 agcgcccgca tcgccaaaat gcagttgagc cgaggcattt gcgcgcgtgc ccgcgtgctt
    27901 ggccaggttg agcatcggaa tatcgacaac agccgcgctc agatcggcct tcaaccccgc
    27961 atccttcaaa ctgaaatgcc gcccatacag cgcgagcgtc accggcattg gccccgtcaa
    28021 ccattgcgga tcggaaagtc cgagcctggc gcgcgacgca tcgtccagcc tcccggtcag
    28081 gtccacccgc gtcgttacgc cgggggcaat aaaatcctcc gtccatttga agctgaccgg
    28141 aacgccttga aggacccctg ttccctgtga cgaaagagat tgggtatcga tggtgaagtt
    28201 gaccatcccg tgttccagct tgcgattgtc gatgggcatg gcaagctcgc tcgccgtcgc
    28261 tttgacgcca atacggagct gatcgagcga aacatccttc agcagcggca acgcaacatc
    28321 caaatcgacc gccgcctgcc cattcacatt cgccgggttg acaccgaagc gtttgggata
    28381 gccgagcggc tcttcgtcga tcagccgcaa tacatcggac atcttgcctt cggaatgcgc
    28441 tttgatccgg gtgataactc cgctgttatg aaggtcggga atgatgacat cgccgccgga
    28501 aagcgcgagc ggcccgaccg aggcggcggc caccgtcaca tggaaactgt cccccgtcag
    28561 ccgcgcttcg ccacgggccc ccgtgatggg cgtcatgccg gcgatatatc gcgcggtcat
    28621 ggcttcgaac ggaaaactca gagtcagagc ttcgtccggc agaaaatctt gatcgagcac
    28681 accggctggg aaatcggcat ccacgcggat ggg
//
LOCUS       DASYEG010000143         5291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3958827, whole genome shotgun sequence.
ACCESSION   DASYEG010000143 DASYEG010000000
VERSION     DASYEG010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3958827"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1458)
                     /locus_tag="VGM72_06410"
     CDS             complement(<1..1458)
                     /locus_tag="VGM72_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEY4264933.1"
                     /translation="MARAAESISTPSRDDFAAMLEESFIERAPAEGAVVKGVIVAVEN
                     DYVVVDVGLKTEGRVPVKEFAAPGQAANVKVGDEVEVYVERVENALGEAVLSRDKARR
                     EESWTRLEKAFQDSGRVTGVIFGRVKGGFTVDLDGAVAFLPGSQVDVRPVRDVGPLMN
                     MPQPFQILKMDRRRGNIVVSRRAVLEEKRAEERSELVASLAEGQVLEGTVKNITDYGA
                     FVDLGGVDGLLHVTDIAWRRVSHPTEVLSVGQQVTVQIIKVNHDTQRISLGMKQLQTD
                     PWEGVGAKYPVDARFRGKVTNVTDYGAFVELEPGVEGLVHVSEMSWVKKNVHPNKIVN
                     PGQDVEVMVLEVDSNKRRISLGLKQCMENPWNAFAENHPGGEVLEGEIRNITEFGLFV
                     GLPGDIDGMVHLSDLSWDKPGDEAVKDYQKGQMVEVKVLDVDPEKERISLGIKQLASD
                     PMAKVSSLKKGDVVTVTVAEVQDGGLEVTLEGGARA"
     gene            complement(1577..2251)
                     /gene="cmk"
                     /locus_tag="VGM72_06415"
     CDS             complement(1577..2251)
                     /gene="cmk"
                     /locus_tag="VGM72_06415"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEY4264934.1"
                     /translation="MSLVIAIDGTAACGKGTLARRLAHHYGFAHLDSGALYRLVALGV
                     VGAGDDPSNVAHALSAAHTIDPAAKDDPRLRSVEIGEAASRVAAIPQVRSAILQYQRD
                     FARFPPGGAPGAVIDGRDIGTVICPDAVAKLFVDARSQVRAQRRWLELKGQNAQPLPD
                     IAAVEADLLARDERDRTRATAPLCQAQDAILLDTSDLDIEAAFAKALDLVNPRIEKAL
                     AARPRG"
     gene            complement(2248..3597)
                     /gene="aroA"
                     /locus_tag="VGM72_06420"
     CDS             complement(2248..3597)
                     /gene="aroA"
                     /locus_tag="VGM72_06420"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEY4264935.1"
                     /translation="MSGLAHQKPLISRLSGPLNGVALAPGDKSVSHRALILGALAVGE
                     TDISGLLEAEDVLNTAAAMRSLGADVIHDAVGHWRVHGVGVGGFAEPADVLDFGNSGT
                     GVRLTMGAVATTPITAIFTGDESLRKRPMRRVLDPLALFGTIAETREGGLLPVTLKGA
                     HQPVPIEYRLQVASAQVKSALILAALNVPGRSTIIEHAATRDHTERMLKAFGADIIVE
                     TIADGSAAISITGEVELHPARIAVPSDPSSAAFPLVAALIVPGSEIVLENVMLNPRRI
                     GLVETLLEMGANIEIRNRRDNGGDAVGDLLVRHSVLRGVDVPPERAPSMIDEYPVLAV
                     AASFAEGRTIMRGLEELRVKESDRLAAVAAGLAANGVRFQESPDGLTVEGAGPDGVAG
                     GGTVATHMDHRIAMSFLVMGLAAKAAVRVDDGSMIATSFPGFQTLMRGLAADITDIP"
     gene            3734..4207
                     /locus_tag="VGM72_06425"
     CDS             3734..4207
                     /locus_tag="VGM72_06425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02300 family protein"
                     /protein_id="HEY4264936.1"
                     /translation="MIKTDLGTKRVCPSCAARFYDLQKRPIECPKCAFAFEPEMLLKQ
                     RRTRVPEPAKPVAAQVEDDEQEEAEDEEAEAEDEELEEEEVVAAADEPQAEAEGEEES
                     EDGGPVAPEEPTEPEEFEEEIEDEEEEDDNLLEEVEEDEDDVSGIIDADIEKDEP"
     gene            4225..4300
                     /locus_tag="VGM72_06430"
     tRNA            4225..4300
                     /locus_tag="VGM72_06430"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4258..4260,aa:Ala,seq:ggc)
BASE COUNT         1017 a         1693 c         1619 g          962 t
ORIGIN      
        1 agcccgcgca ccgccttcga gcgtgacttc cagaccgcca tcctgcactt cggccaccgt
       61 caccgtgacg acatcgccct tcttgagcga ggagactttc gccatgggat cggacgccaa
      121 ttgcttgatg ccgagactga tgcgttcctt ctccggatcg acgtcgagaa ccttgacctc
      181 gaccatctgg cccttctggt agtccttaac ggcttcatcg cccggcttgt cccaggacag
      241 atcggagaga tgcaccatgc cgtcgatatc gccaggcaag ccgacgaaca ggccgaactc
      301 ggtgatgtta cgaatctcgc cttccaggac ttcacctccg gggtgatttt cggcgaaggc
      361 gttccagggg ttctccatgc actgcttcag tccgagactg atgcggcgct tattggaatc
      421 gacctccagc accatgacct cgacgtcctg gccgggattg acgatcttgt tcgggtgtac
      481 gtttttcttc acccagctca tttcggagac gtgcaccagc ccctcaacgc ccggctcgag
      541 ttctacaaaa gcgccgtagt cggtgacgtt agtgactttg ccgcggaagc gcgcgtcgac
      601 cggatatttg gcgcctacgc cttcccaggg atcggtctgc aactgcttca tgccaaggct
      661 gatgcgctgc gtatcgtgat tcaccttgat gatctgcacg gtgacctgct ggcccacgga
      721 cagaacctcg gtcggatgcg agacgcggcg ccaggcgatg tcggtcacat gcagcaggcc
      781 atccacgccg cccaaatcca caaaggcacc ataatcggtg atattcttga ccgtgccttc
      841 cagcacctgg ccttcggcca gcgatgcgac cagttcggaa cgttcttcgg cgcgcttctc
      901 ctccagcacg gcgcggcgcg acaccacgat attgccgcgg cgtcgatcca tcttcaggat
      961 ctggaagggc tgaggcatgt tcatcagcgg gccgacatcg cgcaccggac gcacatcgac
     1021 ctgcgagccg ggcaggaacg ccaccgcccc atccagatcg acggtgaagc cacccttgac
     1081 gcggccgaag atcacgccgg tgacccggcc gctgtcctgg aaggcctttt cgagacgtgt
     1141 ccagctttcc tcgcggcgcg ccttatcgcg cgacaagacc gcctcgccta gcgcgttttc
     1201 cacccgctcg acatacactt ccacctcgtc gccgaccttc acgttcgcgg cctgccccgg
     1261 tgctgcgaat tccttgaccg gaacccggcc ctcggtcttc agcccgacgt ccacgacgac
     1321 gtaatcgttt tcgacggcaa cgatcacacc cttcacaacc gcgccttcgg cgggggcgcg
     1381 ttcgatgaag ctttcttcga gcatggccgc gaaatcgtcg cggctcgggg tggaaatgct
     1441 ttcggcggct ctggccatgg ggtctcctga gttgcattgc ggccgctggg cagcacccat
     1501 acgatccggt ccggcgcaat gccggttcga cgaggaaatg tttcgcaggg tttctctgcg
     1561 gcgccgccgg gcctccttag cccctggggc gggccgcgag cgctttctcg attcttgggt
     1621 tcacaagatc gagggccttc gcgaaggcgg cttctatatc caaatcggag gtatccagca
     1681 agatggcgtc ttgcgcctgg cacaagggcg cggtcgcgcg ggtgcggtct cgttcgtcgc
     1741 gagcgagaag gtccgcctca accgccgcaa tatcgggcag aggttgtgcg ttctggcctt
     1801 tcagctccag ccagcggcgt tgggcgcgaa cctgggagcg ggcatcgaca aagagcttcg
     1861 caaccgcgtc tgggcaaatg accgtaccga tatcgcggcc gtcgatgacc gcgcccggcg
     1921 ccccccccgg cgggaacctt gcgaaatcgc gctgatattg gagaatcgcg ctccgaacct
     1981 gcgggatggc ggcaacgcgc gaggccgcct cgccgatttc gaccgaacgc agcctggggt
     2041 cgtccttcgc ggctggatcg atggtatggg cggcggagag ggcatgggcc acattcgagg
     2101 gatcatcccc ggctcccacc acgccgaggg ccaccagccg gtacagcgcg cccgaatcca
     2161 ggtgcgcgaa accataatga tgcgccagcc ggcgcgcgag ggtgcccttg ccgcaagcgg
     2221 ctgtcccatc gatggcgatg acgagactca cggaatgtcc gtgatgtccg cagccaagcc
     2281 gcgcatcagc gtctggaagc cgggaaaact ggtcgcgatc atggacccgt cgtcgaccct
     2341 caccgccgct tttgcagcaa gacccatcac caggaagctc atggcgatac ggtgatccat
     2401 atgggttgcg acggtgccgc cgcccgccac accgtccggt ccggcccctt cgaccgtcag
     2461 tccgtccgga ctttcctgga aacgcacgcc attcgccgcg agccccgcgg cgacggcggc
     2521 gagccggtcg ctttccttga cccgcaattc ttcgagccca cgcatgatgg tgcgtccttc
     2581 ggcgaaggaa gccgccaccg ccagcaccgg atattcgtcg atcatgctgg gcgcacgctc
     2641 cggcggcacg tccacgcctc tcagcaccga atgacgcacg agaaggtcgc caaccgcatc
     2701 gccgccatta tcgcggcgat tgcggatttc gatgttcgcg cccatttcga ggagcgtttc
     2761 gaccagtcca atccggcgtg gattcagcat cacattctcc agcacgatct cggaacccgg
     2821 cacgatcaag gccgccacca gcggaaaggc ggccgaggac ggatcgctcg gcaccgcgat
     2881 gcgcgcgggg tgcaattcca cctcgccggt gatggaaatg gcggctgatc catccgcaat
     2941 ggtctcgaca atgatgtccg cgccaaacgc cttgagcatg cgctcggtat gatcgcgggt
     3001 ggccgcgtgt tcgatgatgg tggaacgtcc cggcacgttc agcgccgcca ggataagcgc
     3061 ggatttcacc tgagcggacg cgacctgcag ccggtactcg atgggcacgg gctgatgtgc
     3121 gcctttcagc gtgaccggca gcagcccacc ctcgcgggtt tccgcaatcg tgccgaacaa
     3181 ggccagcgga tcgagcacgc ggcgcatggg ccgcttacgg agcgactcgt cgccggtgaa
     3241 aatcgccgtg atcggcgtcg tggcgaccgc acccatggtg agccgaacgc cggttccgga
     3301 gtttccgaaa tcgagcacat cggcgggttc ggcaaaaccg cccacaccca cgccgtgcac
     3361 gcgccagtgc cccaccgcgt catggatgac atcggcaccg agacttcgca tggcggccgc
     3421 ggtgttgagc acgtcttcgg cttcgagcag gccggagatg tctgtttccc ccaccgcgag
     3481 cgcgcccagg atcagcgccc gatgcgacac cgatttgtcg cccggcgcca atgccacgcc
     3541 attcagcggc cccgaaaggc gggaaatcaa cggcttttga tgtgcgagcc ccgacatccg
     3601 ctccctcgtc ttgcgtttgc aactgcctaa accaatgggc gatagggtca agcccgggaa
     3661 gcacgctttg acagcctctc ccgttcatgg caaacccacc gaccctaaag tcacaatgcg
     3721 ggaaaacaac accttgatca aaaccgatct cggcaccaag cgcgtatgcc ccagctgcgc
     3781 agcgcgcttc tacgatctgc aaaagcgccc aatcgaatgc ccgaaatgcg cttttgcgtt
     3841 cgagccggaa atgctcttga aacagcgccg cacgcgcgtg cccgagccgg ccaagcctgt
     3901 ggctgcccaa gtcgaggacg acgagcagga agaggccgaa gacgaggagg ccgaggcgga
     3961 ggacgaggaa ctcgaagaag aggaagtggt tgccgccgcc gacgaacctc aggccgaagc
     4021 cgagggcgag gaagagagcg aggatggcgg tcccgtggcg cccgaggagc ccaccgagcc
     4081 cgaggaattc gaggaagaaa tcgaggacga ggaagaggaa gacgacaatt tgctcgagga
     4141 ggtcgaggag gacgaggacg acgtctccgg cattatcgat gcggatatcg agaaggacga
     4201 accttagccg tcctctcgcg atgtggggcc atagctcagc tgggagagcg cttgaatggc
     4261 attcaagagg tcagcggttc gatcccgctt ggctccacca ataaaatcaa ggacttagac
     4321 aggatttgag gcccgggcaa ccgggccttt ttcgttcggg gtaacaccag gggtaacgcc
     4381 gtcagcagcc cccatccctg ggaggaagcc aggggcatcg gggtggcgaa tgccacgcgg
     4441 caagccgtgg gtgagatttc aggccttcgg agtactgggc cagggggagg ccgggtttgg
     4501 gaccccgggg ccttgcggct gggcatccgc gttcctcgac gttccggtgg tagccgaagc
     4561 accccgattg ggatgcctcc ttggcgcaaa tttggtcagg ccttcgacgc ggaaggctcc
     4621 ggtcttctca tccaagcgcg tgatccgcat aggatcgttc ctaggaaacc aatcgatggc
     4681 ttttctagga tcggccgtta cgtcctttgc tattgctatc actatgcgga caatcccatc
     4741 gcagcgatcg cgcgcccata caagatttct gcggcgctcc gtttcaccac ggcggagact
     4801 ttccttgaga cgcggcacag aattaaatgt tagcgcgtcg ccgtctcgac taagttcgtc
     4861 ctgccatacg gttatcacca cgacctttcc gtcaggcgac cgggcggacc agctccattg
     4921 ggggttgacg cagaccgcac cgaaataggc gaacgccgtt gaccgcttgc tcttgctcat
     4981 aggtatggcc ggaggcttcg ccagccactc tacgcggaca tcattaacct ctcgtcaacc
     5041 gccgagctgc gaactcaaac cgacaggcaa gagcgcggtg cgacacgctt tgaaatgagc
     5101 gttcaccacc ggtaggttta gggaaaagca gacattccgg cccgacaatg agaacgtctg
     5161 gaaatgaccc tggctgtgta aaaacgcgac tttgcgacgg gttgaggacg tgattccctt
     5221 gtctttgcga gtctgatttg cggggatggg atgaagcgat ttgtggcagg agccgatcgg
     5281 gggcaatcga c
//
LOCUS       DASYEG010000144         3578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3379898, whole genome shotgun sequence.
ACCESSION   DASYEG010000144 DASYEG010000000
VERSION     DASYEG010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3379898"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="VGM72_06435"
     CDS             complement(<1..1085)
                     /locus_tag="VGM72_06435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4264937.1"
                     /translation="MAIEALSGWTKEQRSVVAAAYLGWMLDAFDFFLMVFVLRDIAKE
                     FNTEVSSVAVAVVLTLAMRPIGAFICGRAADRYGRRPTLAVVILCYSLLAFTSGFSQN
                     LLQLLILRALFGIAMGGEWGVGASLAMESIPPKARGLVSGMLQAGYPTGYLVASVVYG
                     LLYQYIGWRGMFMIGIVPAFLTYFILKNVKESPAWTPKSVLGSDAIPTLTAYGRLVIY
                     AVLLWVGAGLYANQPGMAALFLAIPIVSSLYLAKRDPGVWIEFFAVIGMLLVDYFSLS
                     THAIPAIMAVIYNVAGILAGVFLKTHWRIGLYAVLLMTGFNFFSHGTQDLYPTFLQQR
                     FSPATVSIIAVIYNIGAILGSLSFGMLS"
     gene            1392..3173
                     /locus_tag="VGM72_06440"
     CDS             1392..3173
                     /locus_tag="VGM72_06440"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEY4264938.1"
                     /translation="MFMWDLSDLYKSPEAWAAEYERVKDEAQGLDAYSQTLGSSAPAM
                     RRALDAISAVNKEASRLYTYASLIADEDLTNSHNQERRQQAGALNTLIGEKTAWLAPE
                     ILTLGSNQVQAFLAADKDLAARHDVFLNNVLRAAPHTLRLEAESVLAAAGDVLQQPSA
                     VYGQFANSDLPYPTVTLSDGTTVRLDQSAYEKYRQSPNRADRKLVFDNFWGAWKNYES
                     TDGAILTAGIMGNVFLAKARKQPNALAAALFPDNMPQEVYRTLVAQANAGLPTLHRYF
                     RLRKQLLGITDALQYYDIYPTMFPGKALPKFDIADSERLTLAAVAPYGAEYRELLERG
                     FAGKWMDAYPRAHKASGAYMNGSAYDLHPYLLLNHNDDYGGLSTFAHEWGHAVHTLLT
                     AKSQPFEKANYSTFIAESASIANEMLLNDYMVAHAKTKEEKLYYLGEGLESIRGTFYR
                     QVMFAEFELALYEEIEKGQPLSGARLTEIYCGLLRKYHGEAEGVMHIDPAYCIEWAFV
                     PHFFYNFYVYQYATSMAGAAEFTDAIVRQGPPAAERFLAMLRAGGSDYPYDLYKRAGI
                     DLAAPGPYRALVGRMNRIMDEIETLRR"
     gene            complement(3177..>3578)
                     /locus_tag="VGM72_06445"
     CDS             complement(3177..>3578)
                     /locus_tag="VGM72_06445"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEY4264939.1"
                     /translation="PDAPEQRNIIGYQVSNQVSVTLDDLSKLGAALDALVKSGANQLG
                     GVSFAIANPKPLADRARALAVAEAMAKAKTLAAAAGVTLGPLLSIQEGTSFRPTPLFE
                     ARAAVAAPAPPPVAIGEQSVTVNVTMTYAIQ"
BASE COUNT          732 a         1082 c         1116 g          648 t
ORIGIN      
        1 gacagcatgc cgaagctcag gctgccgagg atcgcgccga tattgtagat caccgcaatg
       61 atgctgacgg tggccgggga aaaccgctgc tgcaggaacg tcgggtagag gtcctgcgtg
      121 ccgtggctga agaaattgaa acccgtcatc agcaatacgg catagaggcc gatccgccag
      181 tgcgtcttca ggaatacgcc ggccaggatg cccgccacat tgtagatgac ggccatgatg
      241 gccggaatcg catgagtgga gagcgagaaa taatcgacca gcaacatgcc gatcaccgcg
      301 aagaattcga tccacacacc gggatcgcgt tttgcgagat agagactgct gacgatggga
      361 atggccagga agagcgcggc cattcccggc tgattggcat aaaggcccgc gcccacccag
      421 agcagcacgg catagatcac caggcggcca taggcggtca gggtcgggat cgcgtcgctg
      481 ccgagcacgc ttttcggagt ccaggccggc gattccttga cattcttgag aatgaaataa
      541 gtcagaaagg cgggcacgat gccgatcatg aacatgccgc gccagccgat atattggtac
      601 agaaggccgt agacgaccga ggcgaccagg tagccggtcg gatagccggc ctgcaacatg
      661 cccgaaacga gcccgcgcgc cttgggcggg atgctctcca tcgccagtga cgcgcccacg
      721 ccccactcgc cgcccatggc gatgccgaag agcgcgcgca ggatcagcaa ttgcaggagg
      781 ttctgcgaaa agccggacgt gaaagccagc agcgaatagc agaggatgac gaccgccaaa
      841 gtgggccgcc ggccatagcg gtcggccgcg cgcccgcaga tgaacgcgcc gatggggcgc
      901 atggccagcg tcagcaccac cgcgaccgcc accgaggaaa cctcggtgtt gaattccttg
      961 gcgatgtcgc gcagcacgaa caccatcagg aagaagtcga aggcatccag catccagccg
     1021 agataggcgg cggcgacgac gcttctttgt tccttggtcc aaccggacag ggcttcgatg
     1081 gccatggctc tcccccgggc ttaagccctt tcgcatgttt gaacacattc ttagccggtt
     1141 gaagcccgag cgccagtcct ggtttgtggg gcccatctaa attcgccgcg agcgcggttt
     1201 tggccggcag gctcttgggt taaggtgcgc ggctcttaac agggcgtggg ctggcgatta
     1261 tggctttttt cctccggcgc gcatgtttgc tcctgtgcgt gacgctgacg ttgtgtgtga
     1321 cggacgccat gctccagccg ggcggcgcgg ccgcgcaagc gccaaaaagg acaggcatgt
     1381 taaagacagg catgtttatg tgggaccttt ccgatctcta caaatcgccc gaagcctggg
     1441 cggccgaata tgagcgcgtg aaggacgagg cccaaggtct cgatgcctac agccagacgc
     1501 tgggatcgag cgcgcctgcc atgcgtcggg cgctggatgc gatctcggcg gtcaacaagg
     1561 aagcgagccg gctctacacc tacgcgagcc tgatcgccga cgaagatctc accaactcgc
     1621 acaatcagga gcgcagacag caggccggtg cattgaacac gctgatcggc gaaaagacgg
     1681 cttggctggc gcccgagatt ctcaccttgg gatcgaacca ggttcaggct ttcctcgcgg
     1741 cggacaagga ccttgccgcg cggcacgatg tcttcctgaa caatgtgctg cgcgcagccc
     1801 cccacaccct gaggctggaa gcggaatcgg tcctggcggc ggcgggcgac gtgttgcagc
     1861 agccgagcgc cgtatatggc caatttgcga attccgatct gccctatccg acggtcaccc
     1921 tgtcggacgg aacgactgtg cgtctggatc aatccgcgta tgagaaatac cgccagtccc
     1981 ctaatcgcgc cgaccgcaaa ctggtgttcg ataatttctg gggggcatgg aagaattacg
     2041 aaagcaccga tggcgcgatc ctgaccgccg gcatcatggg caatgtgttc ctggcaaaag
     2101 cgcgcaaaca gcccaacgcg ctggccgcgg cactattccc cgacaacatg ccgcaagagg
     2161 tgtaccgcac gctggtggcc caggcgaatg ccggcctgcc gacgctgcat cgctatttcc
     2221 ggttgcgcaa gcaattgctc ggcatcacgg atgcgctcca atattacgac atctatccga
     2281 cgatgtttcc cggcaaagcc cttccgaaat tcgacatcgc ggattccgag cggctcacgc
     2341 tcgccgcggt cgcgccctat ggcgcggaat accgggaatt gctggagcgc ggtttcgccg
     2401 gcaaatggat ggatgcctat ccgcgcgccc ataaggcgtc gggcgcctat atgaacggtt
     2461 cggcctatga tctgcatcct tatctgctgc tcaatcacaa tgacgattac ggcggcttgt
     2521 ccaccttcgc gcatgaatgg ggccatgcgg tccatacgct gctgaccgcc aagagccagc
     2581 cgttcgagaa ggcaaattat tccacgttca ttgccgagtc cgcgtccatc gccaacgaga
     2641 tgctgctgaa cgactacatg gtcgcccacg cgaagaccaa ggaggagaaa ctctattatc
     2701 tcggcgaagg tctcgaatcg atccgcggca cgttctaccg ccaggtgatg tttgccgaat
     2761 tcgaactcgc cctttatgag gagatcgaaa aaggccagcc gctatcgggg gcccggctga
     2821 ccgagattta ttgcgggctt ttgcggaaat atcacggcga ggccgaaggc gtgatgcaca
     2881 tcgatcccgc ctattgcatc gaatgggcgt tcgttccgca tttcttctac aatttctatg
     2941 tctaccaata tgcgacctcg atggcggggg cggccgaatt caccgatgcc atcgtcaggc
     3001 aaggccctcc cgcggccgaa cgctttctcg cgatgttgcg cgcgggcggc tcggattatc
     3061 cttacgatct ctacaagcgc gccggcatcg atctggccgc gccgggcccc tatcgggcgc
     3121 tggtcgggcg catgaatcgc atcatggatg agatcgaaac gctcaggcgc taggcgctat
     3181 tgaatggcgt aggtcatcgt cacattgacg gtgacggatt gctcgccgat ggcgacgggc
     3241 ggcggcgcgg gcgcggccac cgcggcgcgc gcttcgaaca gcggcgtggg gcggaacgat
     3301 gttccttcct ggatcgacag cagcggcccc agagtcacgc ctgcggcggc cgcgagcgtc
     3361 tttgccttcg ccatcgcttc ggccacggcc agcgcgcgcg cgcggtcggc gagaggtttg
     3421 ggattggcga tcgcaaacga caccccgccg agctgattgg cgccgctttt gaccagcgca
     3481 tccagcgcgg ccccgagctt cgacaaatcg tccagagtga cgctcacctg attggagacc
     3541 tgatagccga tgatgttgcg ctgttcgggc gcgtcggg
//
LOCUS       DASYEG010000145        19053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3524671, whole genome shotgun sequence.
ACCESSION   DASYEG010000145 DASYEG010000000
VERSION     DASYEG010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 19053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19053
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3524671"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VGM72_06450"
     CDS             complement(<1..641)
                     /locus_tag="VGM72_06450"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HEY4264940.1"
                     /translation="MITQPALYLMEALLPSDLVDKVNAHIDAVRPIANDYASSLVGQI
                     RRDKKSAQLQMDLQQNIPEALAGVIAQIGTEYLRGQGFAAKVTPNDMWSVHSYEGDYN
                     PLHDHGGNTVLGLSSILYLKVPATILEKRPVDYGRAPSLTMATGNCDGFTQFVWGATG
                     IRDFALLRPPTQQYVKPEAGKLLIFPNWLLHRVEPFYGEGERRTLSCNLDVAFT"
     gene            939..2339
                     /locus_tag="VGM72_06455"
     CDS             939..2339
                     /locus_tag="VGM72_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4264941.1"
                     /translation="MTAQAATCALAHATPKQTPGGEEMNLQDSQRVSAAARLDRLPVF
                     SFHRHMMWLLGFCFFFELGDISTFAFSAPAIRAQWGISIGMIGVITSGTFFGMFLGAA
                     SGGWFADRMGRKRALIAAVTWFSLFSLLNAIAWEPIGLFTARLFTGIGLSAMTAIAIT
                     YIAEMFPARARGTFQGRVMVVGLCGIPGSALIARILVPAFPFGWRLVFVWGALALIFP
                     LLARKLEESPRWYERRGEFAAADDILKRIEATAVQEKGALPPPDTLTAAPREGRFTAL
                     FAPNMLPRTLILVAAWIFQTLGFYGFSAWVPTFLVEHGFKLVDSLNWAFAMQCLGVPG
                     ALMASVLSDRWQRKYWITMFAILIATCGILYGLSSEAVFIVTFGGLMTMGIQAFAPLL
                     YAYTAECFPTEIRSSGTGFTYGVGRLSNALGPLLIAFLFVHYGYMTVFVYIAACWLAV
                     AAIIGLAGPLTRGRPL"
     gene            complement(2342..2827)
                     /locus_tag="VGM72_06460"
     CDS             complement(2342..2827)
                     /locus_tag="VGM72_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264942.1"
                     /translation="MRKIATKIAAMGAAVLVAGCAMAPRGPTVRVFPPPYKPFEVFQQ
                     DQDMCEDYAHSQTAGDAEAANSRAVGTAAIGTALGLALGAATGSGRAASFGAAGGAAV
                     GGTIGANQSERANFSLQRRYDIAYAQCMYAKGNQVPGFGRSAPPPLPPGGPDGPPPRR
                     E"
     gene            complement(3016..3528)
                     /locus_tag="VGM72_06465"
     CDS             complement(3016..3528)
                     /locus_tag="VGM72_06465"
                     /inference="COORDINATES: protein motif:HMM:NF020909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HEY4264943.1"
                     /translation="MAISMYRASAPIFVQMLSASSACLDKAQAYAAAKKIDPSVLLQS
                     RLYPDMFPLSRQLQIASDFGKNTMARLAGIDPPKFEDTETTIEQLKARLAKTVAFVKE
                     FKPSQVDGSEDRDITIQLGGQPRQFKGENYLIGFALPNFFFHVTTAYAILRHNGVELG
                     KNDFTRAPEQ"
     gene            3648..5699
                     /locus_tag="VGM72_06470"
     CDS             3648..5699
                     /locus_tag="VGM72_06470"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264944.1"
                     /translation="MRSIVRLHLPVLLSAIAFVSAATAATVPTGLEGSWTAEQTGTGL
                     SNESTLSFTRAGEMLSGTMRSTDGQTPLFDVRETGGIVSFTLVIPGTPYVAIAYRGMR
                     NGDELRLTGSDEAQAVHTLTARRTAPALQQAAALAPSPLPAQAAPPRSSALPEPARPS
                     LPPPIIASTSGRLDGNWTAEQTSPGSPMPAEVRFVLTGDHGAMHVGAADWPLFDVRDT
                     GTGVAFVLVIPGTPYLTVRYAGTLAGDMLDLIGTGADQGVFHLTARRAPAGEVRGLAG
                     PAPASAAEPIPPRVAALNPPSSPRPTPSRSSGPPPKLPLPALRDVAPNTLFKTPPMGW
                     ASRQKLGPGMEDDDIRQAAESLEETNLRTFGYTLVEIDDGWQGVRDAGGVLHAGAKFP
                     DMKALGDYIHSKKLKFGLQVSAAAKSCGGFEGSYGHEAEDAKLFASWGVDDIVYDWCG
                     AETIYPTQAEQQAAYQKMGEALRASGREIAFAMSQNGAFDVARWGATTGANLWRTGPD
                     IRDNWQSMTEAGFSQNGKEANAGPGHWNDPGLLQTGNGGMTPDEDRTQLNLWAVTAAP
                     LMLGNDVRIMTKETLATLGNGEMIAIDQDPLGRAGKRVAQSGSTEVWARPLAGGATAV
                     AFFNRGDQSAAAAVSWQQLGIDGPRRVRDVWRHEDAGMANERYTVFLTAHSSLLLRLS
                     R"
     gene            complement(5710..6963)
                     /locus_tag="VGM72_06475"
     CDS             complement(5710..6963)
                     /locus_tag="VGM72_06475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEY4264945.1"
                     /translation="MRHKDLLAALLISGLTAVPVAAAPAPGNAPVPAAMPDEALRFAA
                     FLRDFRAEALKAGITPATYDRAVSGIHLNPRVEELNEKQPEFVRPVWEYLAGAVSDTR
                     VARGREQIAAHAELFARLQDNYGVPAEILTAIWGLETGYGQNEGNFNLFEALATLAYD
                     GPRVEYGRRQFIAALRIADVEGRDPTTMTGSWAGAFGHTQFVPTTFLEQAVDGDGDGK
                     RDVWNSPADALASAANYLKHSGWRDGESWGEEVRLPDGFPYEMADTDTAKPIAEWARL
                     GVRRMSGEPLNDAMDAAAIFLPAGYRGPAFLVRNNFRAILKYNAATSYALAIGLLSDR
                     LRGAGTIEASWPLDEAPLDESGRTALQEGLTTLGFSTGGADGVLGRQSRAAIRAYQKA
                     RGIPADGFATANLLTRILNERYLAR"
     gene            complement(6977..7207)
                     /locus_tag="VGM72_06480"
     CDS             complement(6977..7207)
                     /locus_tag="VGM72_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264946.1"
                     /translation="MDLSLTTTLAVAVLMFAVFGFANWRARQPAEPLKVRMINYHVVQ
                     MVCIVIILLMAAHLVSLVTGHPFTGQQGGRAF"
     gene            7338..7463
                     /gene="ykgO"
                     /locus_tag="VGM72_06485"
     CDS             7338..7463
                     /gene="ykgO"
                     /locus_tag="VGM72_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012177066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEY4264947.1"
                     /translation="MKVRNSLRSLKKRDKDCRVVRRKGRVYVINKKNPRFKARQG"
     gene            7542..8258
                     /locus_tag="VGM72_06490"
     CDS             7542..8258
                     /locus_tag="VGM72_06490"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4264948.1"
                     /translation="MRSMLLSLLLIAPFATLALAQPLPSDPHQAGPQDQVPDLQLIPP
                     RGPGEAAPRNAPDAQGVPAQPAPQAARSGRPPPKAPETDEQLLTKLSKAQDQRAARPI
                     ERELRARWAHTGSPSADLLLKRTDQAMEAHDFDTAREIVQKLTIIAPNFAEAWHRRAT
                     LATQKDDYEDALASLRRTLALQPKNFAALAELGEILEEYNDKPHALDAYRQAKAIDPF
                     LDGLADRIRDLTKSVEGQGI"
     gene            8275..8697
                     /locus_tag="VGM72_06495"
     CDS             8275..8697
                     /locus_tag="VGM72_06495"
                     /inference="COORDINATES: protein motif:HMM:NF025032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEY4264949.1"
                     /translation="MQLRTSLLPIAIALAGLCSAPASAEPTLVGIWYSPFQPDEPNVM
                     SLIEFKDDGTFHEEFRKCDNGEFVGYQTESGTWSVSDDVEHINADFINGDTATAQADY
                     RIVSLTDTERRIRMDPQGFVFIGHRVMSFEFPHCATGT"
     gene            8707..9138
                     /locus_tag="VGM72_06500"
     CDS             8707..9138
                     /locus_tag="VGM72_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264950.1"
                     /translation="MSRYAIPTLILCVLLTALSLAQAAPAPVSPFVGIWYGVGEPDDP
                     SISYIDSYHADGTFNSEFRKCEKGEVVWRQTESGKWFLMGSVLRMESDRIDGKKAKFD
                     NAYEIETVTHDEFHARLHDPDFLFVEKRIPKFEFPPCYVGA"
     gene            9337..10296
                     /locus_tag="VGM72_06505"
     CDS             9337..10296
                     /locus_tag="VGM72_06505"
                     /inference="COORDINATES: protein motif:HMM:NF021497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HEY4264951.1"
                     /translation="MGLIALCASARAAEGTEEGTFSVLFENDIFFNTDHDYTNGVELA
                     YTTAPQDTPQWAVRAANWLPFFTAQTDVRTRYAIGQNIYTPSDLNTANPPPTARPYAG
                     FLYGAFGLVGDSGTHLDQLQVMLGVVGPDSLAEESQRWIHGIIHDRKPLGWSTQLHNE
                     PGLIVQYERSIKLIPPRSVLGLIFDAEPHYGLAVGNVYDYVNAGAMARFGFNLPADYG
                     PMRIEPSLPGSNFFEPNGTLSAYVFAGVDGRAVARNLFLDGNTFESSRSVQKMNLVGD
                     VVFGAAITFNKVRIAFTHVVRSREYKTQTQQDRFGAIDLSFRF"
     gene            complement(10299..11726)
                     /gene="pyk"
                     /locus_tag="VGM72_06510"
     CDS             complement(10299..11726)
                     /gene="pyk"
                     /locus_tag="VGM72_06510"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY4264952.1"
                     /translation="MRRSRKTKIVATLGPASHTREHIRALFIAGVDVFRINMSHTNHA
                     NLTTLVDTVRAVEAEAGRPISILVDLQGPKLRLGKIDGGEKRLESGKTVTLVRAETAP
                     DPDHLPMPHPEIFAALKPGSVLLIDDGKVRLVAESVTESRIEAAVLHGGLVKNHKGVN
                     LPDTLLPIPALTPKDRSDLDYALSLNIDWLALSFVQRPDDVAELRKACAGRAGVMTKL
                     EKPAAFYALDEIISLSDSVMVARGDLGVELPLEAVPGRQKQIIQAARHAGKPVVVATQ
                     MLESMIHTAVPTRAEVSDVANAVFEGADAVMLSAETASGAYPAEAVAVMDRIAQAVES
                     DPLYRSIIDAQRQAPESTIADALLAAVHDVTHTIQASAIVCWTKSGSTGLRAARERPE
                     APILALTPIKAMARRLNLVWGLHCVETEDAHDLDDVAERACEIAFTHGFAKQGERVVI
                     TAGLPLGTPGATNMLRVAFVTRKNN"
     gene            complement(11862..12266)
                     /locus_tag="VGM72_06515"
     CDS             complement(11862..12266)
                     /locus_tag="VGM72_06515"
                     /inference="COORDINATES: protein motif:HMM:NF018037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1036 domain-containing protein"
                     /protein_id="HEY4264953.1"
                     /translation="MSSILLPAVTATLLGLSAGPAAAKLSVCNKTAYPASIALGFFNG
                     KQWASSGWWKIDAGNCATLIEDPLLARFYYVYAEHEELGGAWEGDRSFCVGSGHFTIV
                     GRADCLTHGYDVKRFFQVDTGNAPDWTENLAD"
     gene            12374..13504
                     /locus_tag="VGM72_06520"
     CDS             12374..13504
                     /locus_tag="VGM72_06520"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HEY4264954.1"
                     /translation="MTPSFAAEPYELRNGDAPGSLLFLCDHAANTVPQELGTLGLPPP
                     DFATHIAYDIGAAGLTRALADRFGAPAILAHWSRLVVDLNRGADDPTVVMKLSDGRII
                     PGNRDGGRAEIDERIACYHTPYHAAIAARLAAARFQGIVPVLISIHSFTPVMKGIARP
                     WHIGILWDRDARLARPLLARLHAESDIVTGDNEPYSGELENDCLYRHGTMNGLPHVLI
                     EVRQDLIGDEAGLMRWAARLEIILREALAAMGTPAIEFTRPLRVPPTRISEEPVSGGS
                     VMDEKTRTELEAAAFRRLVAHLRNRTDVQNIDLMNLAGFCRNCLGDWYREAAAEKDIV
                     LEKDQAREIVYGMAPAEWKKRYQRESSAEQQAQFAKAQKTHS"
     gene            13604..13849
                     /locus_tag="VGM72_06525"
     CDS             13604..13849
                     /locus_tag="VGM72_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HEY4264955.1"
                     /translation="MAKSGFAAGQLKSIVERIERLEEEKAALGADIREVYAEAKGNGF
                     DTKIIRQVIRMRRLDKADRQEQEAVLDLYLSALGMRS"
     gene            13862..14176
                     /locus_tag="VGM72_06530"
     CDS             13862..14176
                     /locus_tag="VGM72_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264956.1"
                     /translation="MALWVVLASSLPGLAWAAENPQSAKSIGVATMLPNGTILVGVGD
                     TDSDTRARAVLMVEPGDSTYQSIIDHVGGLKPGETKPIPPWPDPPPPKKDTPKNAPPP
                     GA"
     gene            complement(14183..15694)
                     /locus_tag="VGM72_06535"
     CDS             complement(14183..15694)
                     /locus_tag="VGM72_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY4264957.1"
                     /translation="MTQTVLIVDDDPVQRRLLETAVARMGLETLTAPGGGPALDLLFS
                     PKGEQVALVLLDLMMPDVDGIQVLAKLRAVNQSVAVIVLTAKGGIDSAVEAMRAGAND
                     FLVKPASPERIRVSMQNALKLGVLTGEVSRLKKKQHNRLVFDDLIAKSAGMKQVVRIG
                     TRAAQSTIPILIEGESGVGKELVARAIQGGSDRAGKPFVTLNCGAIPDNLIESILFGH
                     EKGSFTGATDKHLGKFLEADGGTLFLDEIGELRLDMQVKLLRALQEGEIDPVGAKRPV
                     KVNVRIMSATNRDLAELTREGRFREDLYYRLNVFPVFIPPLRERREDVPALAEYFMAR
                     FAVEENKRIVGFTPAGLDLLNSYSWPGNVRQLENTIFRAVVLCDDDMLDVADFPQIAS
                     AMGISPREKTHEPGQIPAAEIMASVRVQTQSRAASPYAIGTTDASGHLRKLEELETEI
                     IRMAIERYRGRMSEVARRLGIGRSTLYRKLKDLGLENDEAEAEDESLPRTAAG"
     gene            15933..16100
                     /locus_tag="VGM72_06540"
     CDS             15933..16100
                     /locus_tag="VGM72_06540"
                     /inference="COORDINATES: protein motif:HMM:NF019452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HEY4264958.1"
                     /translation="MAEQHPTSDDHSAGGTMDITDHVKMWNAFWNTSKWGVVGVIILA
                     ILLFTFRTHNG"
     gene            16150..17283
                     /locus_tag="VGM72_06545"
     CDS             16150..17283
                     /locus_tag="VGM72_06545"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEY4264959.1"
                     /translation="MILAIPKERRADETRVAATPETVKKLKGLGLDVVVETGAGAAAR
                     FSDTDYQSAGAAIAPDAASALKNADIVLKVRGPSQEEIAPFKRGAILAALLSPATEKT
                     AIAGLAEAGVIAFAMEFIPRISRAQAMDVLSSQANLAGYKAVIDAAAQFGRAMPMMMT
                     AAGTIAPARVLVMGAGVAGLQAIATARRLGAIVSATDVRPATKEQVESLGATFVAVMD
                     DEFKNAQTAAGYAKPMSEEYQKKQAALIAETIKKQDIVITTALIPGRKAPVLVTEDML
                     KSMKPGSIIVDLAAEQGGNCPLTKPEEVTEVHGVTLMGYCNLPGRLAVDSSSLYARNL
                     FNFVSLFVDKKTGALQLNWDDEIIKGAGLTKDGAIVHPALQPK"
     gene            17295..17603
                     /locus_tag="VGM72_06550"
     CDS             17295..17603
                     /locus_tag="VGM72_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004664154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HEY4264960.1"
                     /translation="MEAIDPFVFRFSIFILAIFVGYFVVWGVTPALHTPLMSVTNAIS
                     SVIVVGALIAVSVPAVSNESWLSKGLGFVALVLASVNIFGGFLVTQRMLAMYKKKEKK
                     "
     gene            17614..19032
                     /locus_tag="VGM72_06555"
     CDS             17614..19032
                     /locus_tag="VGM72_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEY4264961.1"
                     /translation="MNANIAALLYLIAGVLFILALRGLASPETARNGNRRGMIGMALA
                     ILTTLAVTGVSDVLTWALVLAGLGLGGAIGALTARRIAMTAMPQLVAAFHSLVGLAAV
                     LVAGAALYSPDAYGICVHDAANMCTGLIKTQSLVEMSLGVAIGAITFAGSIIAFAKLN
                     GNMSGAPILLPARHLLNLVIALAIVALVGYFVMVQAPWTFWAITVLSFIFGITLIVPI
                     GGADMPVVVSMLNSYSGWAAAGIGFTLENTALIITGALVGSSGAILSYIMCKGMNRSF
                     ISVIAGGFGGETAVAGGVKETRPVKQGSAEDAAFIMKNAGSVIIVPGYGMAVAQAQHA
                     VREMADKLKHEGVKVSYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEDINSQFAQTD
                     VAYVIGANDVTNPAAKNNPQSPIFGMPILDVEKAKTVLFIKRGMGSGYAGIENELFFR
                     DNTMMLFADAKKMTEEIVKALG"
BASE COUNT         3580 a         6045 c         5810 g         3618 t
ORIGIN      
        1 gtgaatgcga cgtccaggtt gcaggacagc gtgcgtctct cgccctcgcc atagaacggt
       61 tcgacccggt gcaacagcca attggggaag atcagcaatt ttccggcttc gggtttgaca
      121 tattgttgag tcggcggacg cagcagcgcg aagtcccgta tgccggtcgc gccccagacg
      181 aattgcgtga acccgtcgca atttccagtc gccatggtca ggctcggtgc gcggccataa
      241 tccaccggcc gcttttccaa aattgtcgcc ggaaccttca ggtacagaat cgacgacaag
      301 cctagaaccg tattgccgcc atgatcgtgg agcggattgt agtcgccttc gtagctgtga
      361 acgctccaca tgtcgttggg cgtgaccttc gcggcgaagc cttgccccct cagatattcc
      421 gttccgattt gcgcgatcac accggcgagg gcttcgggaa tgttctgctg cagatccatc
      481 tgcaactgtg ccgatttctt gtcacgtctg atttgaccga ccagggaaga cgcataatcg
      541 ttcgcgattg gtcgcaccgc atcgatgtgt gcattgacct tgtcgaccag atccgatgga
      601 agaagcgcct ccatgagata cagagcaggc tgcgtgatca tgcggcacgc aatttgctcg
      661 tttaccaact cacgcatacc aactccgtaa cgcctgcgat cttcgaacca ccgggctgaa
      721 acaatcgccg aaccggaggg tgatcaaatg tttaattcac agcatcgtcg cttgagacaa
      781 ccatcgatgc gacacccagc gtgcttatcc cgacagtacg cataaggaca agtttcccct
      841 taccgcacgc gatcaatttt ctttgcggtg ccggcttttg tcgggcaaaa tcctacaagg
      901 gacgcggccc aaccagatgt cacgctcccg tgagttgcgt gacggcgcag gctgcgactt
      961 gtgcgctcgc gcatgctacg ccaaaacaga cgccgggggg cgaggagatg aacctgcagg
     1021 atagccaacg tgtgtcggcc gcggcgcggc tagaccggct gccggtcttt tccttccatc
     1081 gccatatgat gtggctgttg ggcttctgct tcttcttcga actcggggac atcagtacgt
     1141 ttgcgttctc cgcgccggcg atccgcgccc aatggggcat ctccatcggc atgatcgggg
     1201 tcatcacctc gggcacgttc ttcgggatgt tcctgggagc ggcaagcggc ggctggttcg
     1261 ccgaccgcat ggggcgcaag cgcgcgctca ttgccgcggt gacgtggttc agcctgttct
     1321 cattgctgaa tgcaatcgcc tgggagccca tcgggctttt caccgcccgg ctgttcaccg
     1381 gcattgggct ttcggcaatg acggcaatcg ccattaccta tatcgccgaa atgtttcccg
     1441 cccgcgcgcg cggcacgttc cagggccggg tcatggttgt cggcctttgc ggcataccgg
     1501 gctcggctct tattgcgcgg attctggttc cggcgtttcc gttcggctgg cggctcgtct
     1561 tcgtgtgggg agcgctcgcg ctgatcttcc ccctgctggc acgcaagctc gaagaatcgc
     1621 cgcgctggta tgagaggcgc ggagaattcg ccgccgccga tgacatcctg aaacggatcg
     1681 aagctaccgc ggtacaagaa aagggcgcac tgccgccgcc cgatactctc acggcagcac
     1741 cgcgcgaagg acgtttcacc gcgctgtttg cgcccaatat gctgccgcgc acgctgatcc
     1801 tggtcgcggc ctggatattc cagacactcg gcttctatgg cttttccgcc tgggttccga
     1861 cattcctggt cgagcacgga ttcaagctcg tggattcctt gaactgggcc ttcgccatgc
     1921 aatgtctcgg agttcccggc gccctcatgg cttcggtcct ttcggatcgc tggcagcgca
     1981 aatattggat cacgatgttc gcgattctga tcgcaacctg tggaatcctt tatggactca
     2041 gctccgaggc ggtgttcatc gtcactttcg gcggcctgat gaccatgggc attcaggcgt
     2101 tcgcgccact actttatgcc tataccgccg aatgtttccc caccgagatc cgcagctccg
     2161 gcaccggctt cacctatggc gtcggacggt tatccaatgc gttggggcca ttgctgatcg
     2221 cgttcctgtt cgtccattac ggttacatga ccgtgttcgt gtacatcgcg gcgtgctggc
     2281 tggccgtcgc cgcgattatc ggactggccg gaccgctcac gcgtggccgc cccctttagg
     2341 ctcactccct acgcggcggc ggtccgtcgg ggcctccggg ggggagggga ggaggcgcgg
     2401 aacggccaaa gccgggaacc tgattgccct tggcatacat gcactgcgca taggcgatgt
     2461 catagcgccg ctgcaggctg aaattcgccc gttcggattg gttcgcgccg atggtgccgc
     2521 cgacagcggc gccacccgcc gcgccgaagc tcgcggcgcg gccactgccg gtcgctgcgc
     2581 ccagcgcaag acccagcgcg gttccgatgg ccgccgttcc caccgcacgg ctgttggccg
     2641 cttccgcgtc gccggcggtc tggctgtgcg cataatcctc gcacatgtcc tgatcctgct
     2701 ggaacacttc gaaaggctta tagggcggag ggaaaacgcg caccgtcggg ccgcgcggtg
     2761 ccatcgcgca ccccgcgacc agcacagccg cgcccattgc cgcaatcttc gtcgcaatct
     2821 tacgcatgaa cgactcccgg atcgcaggga cccgccaggg gaggcgtctc gcctcgcatc
     2881 agggaccggc tcccattttt catatcggat cgtcttgtcg cacgcacaag ctgaaccgcc
     2941 gatgaattgc agcgcggtgc ccatcccaca ccggacccaa cagcgtcagc tcgacagtct
     3001 catgatcggc cgcgttcact gttccggagc gcgcgtaaaa tcgtttttgc cgagttcgac
     3061 gccattatgg cgcagaatcg cgtaggcggt ggtgacgtgg aagaagaagt tcggcagcgc
     3121 gaacccgata aggtaattct cgcctttgaa ctggcgcggc tgcccaccca gttggatcgt
     3181 gatatcgcgg tcttcggaac catcgacctg ggaaggcttg aactccttga cgaaagcgac
     3241 cgtcttggcg aggcgcgcct tcaactgctc aatggtggtt tcggtgtcct cgaatttcgg
     3301 cggatcgatg ccagcgaggc gcgccatcgt attcttgccg aaatccgatg cgatctgcaa
     3361 ctgacgcgac agcgggaaca tgtccgggta aagccgcgat tgcagcagca cggacggatc
     3421 gatcttcttt gctgccgcat aagcctgggc cttatcgaga caggccgacg aagccgaaag
     3481 catttgcacg aaaatcggcg ccgaagcgcg gtacatagag atagccatgg gaactccctt
     3541 ggaaaggatg tggggatatt ttttcagtgt atctgcgttt gccgtccatg cttagttagc
     3601 cgaacggtca tgggtcataa agtgacaatc gcagcaagga tgagagcatg aggtcgatcg
     3661 ttcggctgca tctgccggtc cttttaagcg cgatcgcgtt tgtctcggcc gccacggccg
     3721 cgacggtccc aaccgggctc gaaggcagtt ggaccgccga acagactgga accggtctct
     3781 ccaatgaatc gacactgagc ttcacacgcg cgggcgaaat gctcagcgga accatgcgca
     3841 gcaccgatgg ccagaccccg ctgttcgatg tgcgtgagac gggtgggatt gtttccttca
     3901 ccctcgtgat ccccggcacg ccctacgtcg caatcgccta tcgcggaatg cgcaatggcg
     3961 atgagcttcg cctgaccgga tcggacgagg cacaagccgt ccacaccctg acggcgcgac
     4021 ggaccgcgcc tgcgctccag caagccgcgg cactcgctcc aagtcctctt ccggcacaag
     4081 ccgcgccgcc gcggtcatcc gcattgccgg aaccggcgcg gccatccttg ccgccgccca
     4141 ttatcgcgag cacgagcgga cgcctcgacg gcaattggac cgccgagcag acgagccccg
     4201 gctctcccat gcccgccgaa gtgcgcttcg tgcttaccgg cgatcacggc gcgatgcatg
     4261 tgggcgcggc cgattggccc ttgttcgacg ttcgcgatac cggcaccggc gtggcctttg
     4321 tgctggtgat ccccggtacg ccttatctca ccgtccgcta tgccgggacg cttgcagggg
     4381 acatgctgga tctcatcggc accggtgcgg atcagggcgt gtttcatctg acggcacgcc
     4441 gcgcgccggc cggcgaagtt cgcggactcg ccgggccggc gcccgcctcc gctgcagaac
     4501 caatcccacc gcgcgtcgcg gctctcaatc cgccatcgtc gccacgcccc acgccgtcgc
     4561 gttcctccgg tcccccgcca aaacttccct tgccggcctt gcgtgacgtc gcgcccaaca
     4621 cgcttttcaa gacgccgccc atggggtggg cgagccggca aaaactcggt cccggcatgg
     4681 aagacgatga catccggcaa gccgcggaga gtctcgaaga aaccaatttg cgcacattcg
     4741 gctacacgct cgtcgagatc gacgatggct ggcaaggcgt gcgcgatgcg ggcggcgtgc
     4801 tgcacgcggg cgcgaaattc cccgacatga aggcgctcgg cgattacatc cattcgaaga
     4861 agctgaaatt cggcctgcag gtttccgccg ctgcgaaaag ctgcggcggc ttcgaaggca
     4921 gctatggcca tgaagccgag gatgcgaaat tgttcgcgtc ctggggcgtc gatgacatcg
     4981 tctacgattg gtgtggcgcc gaaaccattt atccgacgca ggccgagcaa caggctgcct
     5041 atcagaaaat gggtgaggcc ttgcgcgcgt cggggcgcga gatcgcgttc gccatgagcc
     5101 agaacggcgc cttcgacgtc gcgcgctggg gcgcgacgac cggcgccaat ctgtggcgca
     5161 ccggacccga tatcagggac aattggcaat cgatgaccga agccggcttc agccagaacg
     5221 gcaaggaagc caatgccggg cccggccact ggaacgatcc cggtctgctt caaactggca
     5281 atggcggcat gacgcccgat gaagaccgca cgcagttgaa cctgtgggcc gtcacggccg
     5341 ctcctctcat gctcggcaat gatgtgcgca tcatgaccaa ggaaacactc gcgacactcg
     5401 gcaacggcga aatgatcgcg atcgatcagg acccgctcgg gcgtgcaggc aagcgcgtgg
     5461 cgcagagcgg atcaaccgaa gtatgggcgc gtcccctcgc cggcggggcg acagcggtcg
     5521 cctttttcaa tcgtggagat cagagcgcgg cggccgcggt gtcctggcag cagctcggca
     5581 tcgatggacc acggcgcgtg cgcgatgtct ggcgtcatga ggatgctggc atggcgaatg
     5641 agcgctatac cgtattcctc accgcccatt cctcgctgct gctgcggctc agccggtagt
     5701 agatggagct cagcgtgcga gatagcgttc gttcaggatg cgcgtgagaa gattcgccgt
     5761 tgcaaatccg tccgccggaa tgccgcgcgc cttctgatac gcgcgaattg ccgcacgcga
     5821 ctggcgcccg agcacgccgt ccgcgccgcc tgtcgaaaac ccgagtgtcg tcaatccttc
     5881 ctgcagcgcc gtgcggcccg attcatcgag cggcgcttcg tcgaggggcc agcttgcctc
     5941 aatcgtgccg gcgccccgca atcgatccga caggagcccg atggcaagcg cataggacgt
     6001 ggccgcgttg tatttcagga tcgccctgaa attgttgcgc accaggaagg ccgggccgcg
     6061 atagcccgcc ggcagaaaga tggcggcggc atccatggcg tcgttcaggg gctccccgga
     6121 catcctgcgc actcccagcc gcgcccattc ggcgatcggc ttggcggtat cggtgtcggc
     6181 catctcatag gggaacccgt ccggcaaacg cacttcttcg ccccaggatt cgccgtcacg
     6241 ccagcccgaa tgcttcaggt aattcgccgc cgatgcgagc gcatcggcgg gcgagttcca
     6301 gacatcgcgc ttgccgtcgc cgtcgccatc caccgcctgt tcgagaaacg tcgtgggcac
     6361 gaactgcgtg tgcccgaaag cgcccgccca tgagcccgtc atggtcgtgg gatcgcgtcc
     6421 ctccacgtcg gcgatgcgca gggcggcgat aaactgccgg cggccatatt cgacccgcgg
     6481 cccgtcatag gcgagcgtgg ccagcgcctc gaacagattg aaattgcctt cgttctggcc
     6541 gtagcccgtc tcaagtcccc agatcgcggt caggatttcg gcgggcacgc cataattgtc
     6601 ctgaaggcgg gcaaacaatt cggcatgggc cgcgatttgc tcgcgccccc gggcgacccg
     6661 tgtgtccgaa accgctcccg ccagatattc ccagaccggg cggacgaatt ccggctgttt
     6721 ctcgttcagt tcttcgacgc gggggttgag atggatgcca gacaccgcgc gatcgtacgt
     6781 tgccggggtg attccggctt tcaaagcctc ggcgcggaaa tcgcgcagga aggccgcgaa
     6841 gcgcagcgcc tcgtccggca tcgccgcggg gaccggggca ttcccgggcg cgggggcagc
     6901 cgccaccggc acagcggtca aaccgcttat caaaagggcc gccaaaaggt ctttatgccg
     6961 catcgcaagc ctcacgctag aatgcccttc cgccctgctg accggtgaag ggatgcccgg
     7021 tgacaagcga gaccagatgg gccgccatca aaaggataat gacgatgcaa accatctgga
     7081 ccacgtgata attgatcata cgcaccttga gcggctcggc cggctggcgt gcccgccaat
     7141 tggcaaatcc gaaaaccgcg aacatcaaca ccgcaacggc cagcgtcgtg gtcaaactca
     7201 aatccatgcc ccactccctg tgattcgtgc aaaaacgccg gttccagccc agcgaggtgg
     7261 ttgacagccc ccagtcgcgt gcataggttc ccgcgccttt cggcagattt gacggcatct
     7321 tagcaccgga gaccatgatg aaagtgcgca attctctgcg ctcgctgaaa aagcgcgata
     7381 aggactgccg cgtcgtccgc cgcaagggcc gcgtctacgt catcaataag aaaaaccccc
     7441 gtttcaaggc tcgccagggc tgatttcgag ggcggatttc gtttaggcgt ttgtgttttt
     7501 gcgcctatcg cctcggtcag attttttggc tagcatgggc catgcgctcg atgctcctga
     7561 gcttgctgct cattgcgcca tttgccacat tggccctggc gcagcctctg ccgtccgatc
     7621 cgcatcaggc cggtccccag gaccaggttc ccgatctgca actgattccg ccgcgcgggc
     7681 cgggcgaggc cgcgcccagg aatgcgccgg acgcgcaagg cgtcccggcc cagccggcgc
     7741 cccaggccgc ccgctccgga cggccgcccc ccaaggctcc ggaaaccgac gaacagcttc
     7801 tgacgaagct cagcaaggcg caggaccagc gcgccgcgcg tcctatcgag cgggagttga
     7861 gagcgcgctg ggcccatacc ggtagcccga gcgccgacct tctgctcaag cgcaccgatc
     7921 aggcgatgga agcgcatgat ttcgacaccg cgcgcgagat tgttcagaag ctgaccatca
     7981 tcgcgccgaa tttcgcggaa gcctggcacc ggcgcgcgac gctcgccacc caaaaggacg
     8041 attatgagga cgcgctcgcg tctctgcgcc gcacgcttgc gctgcaaccg aagaattttg
     8101 ccgctctcgc tgaactgggc gaaatcctgg aggaatacaa cgacaagccc catgcgctcg
     8161 atgcttatcg gcaggccaag gccatcgatc ccttcctgga tggcctggcg gaccgcatcc
     8221 gcgatctgac caagagcgtc gaaggtcagg gaatctgatt cgatttcgaa cccgatgcaa
     8281 ctcaggacct cgcttctccc catcgcaatc gcgctcgcag gcctgtgctc agcccccgcg
     8341 agcgcagagc ccacattggt cggcatctgg tacagcccgt tccagccgga cgagcccaat
     8401 gtgatgtcgc tgatcgagtt caaggacgac ggcacgttcc acgaagaatt ccgcaaatgc
     8461 gacaatggcg agttcgtcgg ctatcagacg gaatccggca cctggtcggt gtcggacgat
     8521 gtcgagcaca tcaacgcgga tttcatcaat ggcgataccg ccaccgccca ggccgattac
     8581 agaatcgtct cgcttaccga cacggaaagg cgcattcgca tggaccctca aggcttcgtc
     8641 ttcatcggcc atcgggtgat gagctttgaa ttcccgcatt gcgccaccgg cacctaggtc
     8701 atcatcgtgt cccgatacgc cattccaaca ttgatcctct gcgtcttgct caccgcgctg
     8761 tctttggcgc aagcagcccc ggcgccggta tctccctttg tcgggatttg gtacggcgtc
     8821 ggagaacccg acgatcccag catctcctat atcgactcct atcatgcaga cggaaccttc
     8881 aattcggagt tccgaaaatg cgagaagggc gaagtcgtct ggcggcagac cgagagcggc
     8941 aaatggtttt tgatgggcag tgtcctcagg atggagtcgg accggatcga cggcaagaag
     9001 gcgaagttcg ataatgcgta tgagatcgag acggtgacgc atgacgaatt ccacgcacgg
     9061 cttcacgatc ccgacttcct gttcgtcgag aagcgcattc caaagttcga atttccgccc
     9121 tgctatgtgg gtgcctgaga tttaggcggt tcgccgggcc tcacgccgcg cgggtaaacg
     9181 tcctccccac catcgcgctt cccggaacca agcgctgttc cggcggttat ggtcgccgtt
     9241 ggaacgggaa tgattcaggc gcggctgcgc cacgaattcg gggccatttt gatagcgaag
     9301 atcttatgtc ccgtggcgcg ggccacggcg ctggccatgg gtctgatcgc cctgtgcgca
     9361 tctgcgcgcg ccgcggaagg aacggaagaa ggcacgttca gtgtcctctt cgagaacgat
     9421 atcttcttca acaccgacca tgactacacc aacggggtcg aactggccta cacgacggcc
     9481 ccgcaggata cgccgcaatg ggcggtccgg gccgcaaatt ggcttccttt tttcaccgcg
     9541 caaaccgatg tgcgcacgcg ctacgccatc ggccagaaca tctacacacc gagcgatctg
     9601 aacacggcga acccgcctcc tacggcgcgt ccttatgcgg gatttctcta cggcgcgttc
     9661 gggctcgtgg gcgacagcgg cacgcatctg gaccagttgc aggtcatgct cggcgtcgtg
     9721 gggccggatt cgctggccga agaaagccaa agatggattc acggaatcat ccacgacagg
     9781 aaaccgctcg gctggtccac gcaactccac aatgaaccgg gcctcatcgt ccaatacgaa
     9841 cgcagcatca agcttattcc gccgcgatcc gttttaggcc tgatcttcga cgcggagccg
     9901 cattacggct tggcggttgg caatgtgtat gactacgtca atgctggcgc gatggcgcgt
     9961 tttggcttca atctgccggc cgattatggc ccgatgcgca tcgagccgag cctgcccgga
    10021 tccaatttct tcgagccgaa cggcacgctg agcgcgtatg ttttcgccgg tgtggatggg
    10081 cgtgcggtcg cgcgcaatct ctttctcgac ggaaatacct tcgagtcgag ccgcagcgtc
    10141 cagaagatga atttggtcgg cgatgtcgtg ttcggcgcag ccatcacctt caacaaggtg
    10201 cggatcgcct ttacgcatgt cgtccgcagc cgtgagtaca aaacccagac gcagcaggac
    10261 cggttcggcg cgatcgatct atcgttccga ttctagaact aattgttttt tcgcgtgacg
    10321 aaggcgacgc gcagcatgtt ggtggcgccc ggcgtcccca gcggcagacc ggcggtgatc
    10381 accacacgtt ccccttgctt cgcaaagcca tgggtgaacg cgatctcaca ggcgcgctcg
    10441 gcgacatcgt cgaggtcgtg cgcgtcttcg gtctcgacgc aatgcagacc ccagacgagg
    10501 ttcaaacgcc gcgccatagc cttgatcggt gtcagggcca gaatcggcgc ctcgggacgt
    10561 tcgcgcgcgg cgcgcaggcc cgtcgagccg gacttggtcc agcatacgat ggccgaggcc
    10621 tggatcgtat gggtgacatc gtggaccgcg gccaggaggg catcggcgat ggtggattcg
    10681 ggcgcctgac gttgggcatc gatgatgctg cgataaagcg gatcgctctc gacggcttgc
    10741 gcgatgcggt ccatcacggc gaccgcttcg gccggatagg cgccggacgc ggtttccgcc
    10801 gacagcatca cggcgtccgc gccttcgaag acggcattgg cgacatcgga cacctcggca
    10861 cgggtcggca ccgcggtatg gatcatggat tccagcatct gcgtcgccac caccacgggt
    10921 ttgcccgcat ggcgcgcggc ctggatgatc tgcttctggc ggccgggcac ggcttcgagc
    10981 ggaagctcca cgccaagatc gccgcgcgcc accatgaccg aatcggagag gctgatgatt
    11041 tcgtcgagcg cgtagaacgc ggccggcttt tccagcttgg tcatgacgcc cgcgcgcccc
    11101 gcgcaggctt tgcgcagttc cgccacatcg tcgggacgct gcacgaagga tagcgccagc
    11161 cagtcgatgt tcagggacaa ggcataatcg aggtcgctgc gatccttcgg cgtcagggcc
    11221 ggaatcggca acagcgtgtc ggggagattc acgcccttgt ggttcttgac cagcccgcca
    11281 tgcagcaccg cggcctcgat gcgcgattcg gtaacggatt ccgccaccag acgtaccttg
    11341 ccgtcatcga tgagaagtac cgagccgggc ttcagcgccg cgaagatctc cggatgcggc
    11401 atcggaagat ggtcgggatc gggcgcggtt tccgcgcgca ccagcgtcac cgtcttgccg
    11461 ctttccagcc gtttctcgcc gccatcgatc ttgcccagcc ggagcttggg tccttgcaga
    11521 tcgaccagga tggagatcgg ccgcccggcc tcggcttcca ccgcgcgcac cgtatcgacc
    11581 agcgtggtca gattggcatg attggtgtgg ctcatattga tgcggaacac gtccacgccc
    11641 gcgatgaaca gggcacggat gtgctcgcgc gtgtggctag cgggacccag cgtggcgacg
    11701 atcttggttt tgcggctgcg gcgcatctgt tactggagaa tcctcatatt cgcgcccgga
    11761 ccattcgtgg agaagcgcct tcgcgtttca ctgccgccgg cgcggcagcg caagaatcga
    11821 gaatgctttc gcgattcagg gtaaagtgaa agcgctccag gtcaatccgc caaattctcg
    11881 gtccagtccg gcgcattgcc ggtatccacc tggaagaagc gtttgacgtc atagccatgg
    11941 gtaagacaat cggcgcgccc cacaatcgta aaatggccgc ttccaacgca gaagctgcgg
    12001 tcgccctccc aggcacctcc cagttcttca tgttcggcat aaacataata gaaccgtgcc
    12061 aaaagagggt cctcgatgag cgtggcgcaa ttgcccgcgt cgattttcca ccagcctgag
    12121 cttgcccatt gcttgccgtt gaagaacccc agcgcgatgg aggcggggta ggccgtcttg
    12181 ttgcagacgg agagttttgc ggccgcggga ccggcggata gccccagaag ggttgcggtt
    12241 accgcgggca gaagaatgct ggacaaacgc gcgctcacac acgaaagggt gtcgggacct
    12301 tctcttgctg tcaatgcgct tccgcatgca tttgtgtgcc agatccttgc tccggccacg
    12361 ctatattcga atcatgacac cgagtttcgc cgccgaaccc tatgagctgc gcaatggcga
    12421 cgcaccgggc tccttgcttt tcctatgcga tcacgcggcc aatacggtgc cccaggaact
    12481 tgggaccctg gggctcccgc cgccggattt cgccacccat atcgcctatg acatcggggc
    12541 ggcgggcctg acgcgcgcac tcgcagaccg ctttggcgcg cccgcgatcc tggcgcactg
    12601 gtcccgtctg gtggtcgacc tcaatcgcgg ggcggacgac ccgacggtgg tcatgaagct
    12661 gtcggacggg cgcatcatcc cgggcaatcg cgacggcggc cgggcagaaa tcgacgaacg
    12721 catcgcctgc tatcacacgc cctatcacgc cgcgattgcc gcgcgcctgg ccgcggcgcg
    12781 ttttcagggg atcgtgccgg tgctgatctc catccacagc ttcaccccgg tgatgaaagg
    12841 aattgcgcgg ccctggcata tcggaatcct gtgggacagg gatgcgcgcc ttgccagacc
    12901 cctattggcg cgtctgcacg cggaaagcga catcgtgacc ggcgacaacg agccctattc
    12961 gggtgagctg gagaacgatt gtctttaccg ccacgggacc atgaatgggc ttccccatgt
    13021 gctgatcgaa gtgcgccagg acttgatcgg cgatgaggcg ggccttatgc gctgggctgc
    13081 ccgtttggaa atcatcttgc gcgaagcgct ggcggcaatg ggcacaccgg cgatagaatt
    13141 cacccgcccc ttaagggtgc cgccaacacg gatttcagaa gagccggttt caggaggttc
    13201 cgtcatggac gagaaaacgc gcaccgaatt ggaggccgcg gccttccggc gtctggtcgc
    13261 gcatctgcgc aatcgcaccg acgttcagaa tatcgatttg atgaatctcg ccggcttctg
    13321 ccgcaattgc ctgggcgatt ggtatcgcga ggccgccgcc gagaaagaca tcgtcctgga
    13381 aaaggaccag gcccgcgaga tcgtctatgg catggcgccg gccgaatgga aaaaacgcta
    13441 tcagcgggaa tcttcggcgg aacagcaggc gcaattcgcc aaagcgcaga agactcacag
    13501 ctaaacgcgc cgtttcagct ttccatccgc catcctgcgg tctatccgga cggcccatga
    13561 cggcgcgatc cccgtgccgt gacggcatta tctaggaaca gccatggcaa aatccggctt
    13621 cgcggcagga cagttgaaat cgatcgtcga gcgcatcgag cggctggaag aggaaaaagc
    13681 cgcgctcggc gccgacattc gcgaggtcta tgccgaggcc aagggaaacg gattcgacac
    13741 caagatcata cgtcaggtga tccgcatgcg gcgcctcgac aaagccgatc gccaggagca
    13801 ggaagccgtg ctcgacctct atctttcggc gctgggtatg cgaagctgag gcatggtccg
    13861 cttggcgctc tgggttgttc tggcctcgtc gcttccgggt ctggcttggg ccgcggaaaa
    13921 cccccaaagc gcgaaatcca tcggcgtcgc aaccatgctg ccgaacggaa ccatcctcgt
    13981 gggcgttggg gacacggatt cggatacgcg ggcgcgggct gtcctgatgg tcgaacccgg
    14041 cgacagcacc taccaatcca tcatcgacca cgtcggaggc ttgaagcccg gcgaaaccaa
    14101 acccattccg ccctggccgg acccgccgcc gccgaaaaaa gacaccccca agaacgcccc
    14161 accgccggga gcctgaaacg gatcagccgg ctgccgtgcg cggcagagat tcgtcttcgg
    14221 cctccgcttc gtcattctcg aggcccaagt ccttgagctt gcggtagagg gtggaacggc
    14281 cgatccccag cctgcgcgcc acttccgaca tgcggccgcg atagcgttcg atcgccatgc
    14341 ggatgatttc cgtctccagc tcctcaagtt tgcgcagatg acccgacgca tccgtcgtcc
    14401 cgatggcgta agggctcgcc gcgcgcgatt gtgtctggac gcggacggaa gccatgatct
    14461 cggccgccgg aatttgtccc ggttcatggg tcttttcgcg cggcgagatg cccatggcgc
    14521 tggcgatttg cgggaaatcc gccacgtcca gcatgtcgtc atcgcacagc acgaccgcgc
    14581 ggaaaatcgt attctcgagc tggcgcacat tacccggcca ggaataggaa ttgagcagat
    14641 ccaaccctgc gggcgtgaag ccgacgatcc gtttgttttc ttccacggca aaacgggcca
    14701 tgaaatattc ggcgagcgcg ggcacgtctt cgcgccgttc ccgcaagggc ggaatgaaca
    14761 ccggaaatac attcagccgg taataaagat cctcgcggaa ccgcccctcg cgcgtgagtt
    14821 cggcgagatc gcgattggtg gccgacataa tgcggacatt gaccttcacg ggccgcttcg
    14881 cgccgaccgg atcgatctcg ccttcctgca gcgcgcgcag aagcttgacc tgcatgtcga
    14941 gcctgagttc gccgatctca tcgagaaaca aggtgccgcc gtcggcttcc aggaacttgc
    15001 ccagatgctt gtcggttgcc cccgtgaagg agcccttctc gtgaccgaag aggatgcttt
    15061 cgatcagatt gtcggggatg gctccgcaat tcagcgtgac gaacggtttg ccggcgcgat
    15121 ccgacccgcc ctggatggcg cgcgcgacca attccttgcc gacgcccgat tcgccttcga
    15181 tgaggatggg aatggtagat tgcgccgcac gcgtgccgat ccggacgacc tgcttcatgc
    15241 cggcgctttt ggcgatcaga tcgtcgaaca ccagccggtt gtgctgcttc ttcttgagcc
    15301 gcgagacctc gcccgtgagc acgccgagct tgagggcgtt ctgcatggag accctgatgc
    15361 gctcggggct ggcaggcttg accaggaaat cattggcccc tgcccgcatc gcctcgaccg
    15421 ccgaatcgat gccgcccttg gccgtcagca cgatcaccgc gacgctctga ttgaccgcgc
    15481 gcaatttggc gagcacctgg atgccgtcga catcgggcat catcaaatcg agcaggacga
    15541 gcgcgacctg ctcgcctttg ggcgagaaca gaaggtcgag cgccggcccg cctccggggg
    15601 cggtcagcgt ttccaacccc atccgcgcga cggccgtttc caagaggcgg cgctgtaccg
    15661 ggtcgtcgtc gacgatgaga accgtttggg tcatgaaaag gggcgtatcc tgcggggacg
    15721 atcagcgcct attctcgcgc ccaaaggtaa aagcgggctt aaccgtccca aattgggcca
    15781 cccaatccgg caacggccgc gcacccgagc ggccattcgg cgcgacgaaa ggtcgccctg
    15841 aagctccacc cccggattta tcaggccttt gctgccgctt ttcctttgct atagtcgcgc
    15901 caccattcgc aaacgctgaa acggggacag ccatggccga acaacatccg acatccgacg
    15961 atcattcggc cggcgggacc atggacatca ccgaccatgt gaagatgtgg aacgccttct
    16021 ggaacacttc gaaatggggg gtcgtcgggg tcattatcct cgccatcctt ttgttcacct
    16081 tccgcaccca taacgggtga caatcgcgcc ccagataaag cccccgggcc atatgaaggc
    16141 ctcaaggcca tgatccttgc gatccccaaa gagcgacggg cggacgaaac gcgtgtcgcg
    16201 gccacgccgg aaacggtcaa gaagctgaaa ggtctcggtc tcgatgtcgt ggtcgagacc
    16261 ggtgcgggag ccgcggcgcg tttctccgac accgattatc aaagcgcggg cgcagccatt
    16321 gcaccggacg cggcgtctgc gctgaagaac gccgacatcg tattgaaagt ccgtggacct
    16381 tcgcaggagg aaatcgctcc gttcaaacgc ggcgcgattc tggcggcgtt gctgtcgccc
    16441 gcgaccgaga aaaccgcgat agccggtttg gccgaggcag gtgtgattgc cttcgccatg
    16501 gaattcatac cccgcatcag ccgcgcccag gccatggacg tgctgtcgag tcaggccaat
    16561 ctcgcgggct acaaggcggt gatcgatgcc gccgcccaat tcggccgtgc catgccgatg
    16621 atgatgacgg ccgcaggcac catcgcaccg gcgcgcgtgc tggtgatggg cgcgggcgtc
    16681 gcgggcctgc aagccatcgc gaccgcacgg cggctgggcg ccatcgtctc ggccaccgat
    16741 gtgcgccccg ccaccaagga acaggtggaa agcctgggcg ccaccttcgt tgcggtgatg
    16801 gacgatgaat ttaagaacgc gcagacggcg gcgggttatg ccaagccgat gagcgaggaa
    16861 taccagaaga aacaggcggc cctgatcgct gaaaccatca agaaacagga catcgtcatc
    16921 accaccgcgc tgatcccggg ccgcaaagcc ccggtgctgg tgaccgaaga catgcttaag
    16981 tccatgaagc cgggctcgat catcgtcgac ctcgcggccg aacagggcgg caattgcccc
    17041 ctgaccaagc ccgaggaggt gaccgaggtc catggcgtga cgctgatggg atattgcaat
    17101 cttcctgggc ggctcgcggt ggattcgtcc agcctgtatg cgcgcaacct cttcaatttc
    17161 gtgagcctgt tcgtcgacaa gaagacgggc gcgcttcaac tgaattggga cgacgagatc
    17221 atcaagggcg ccggcctgac caaggatggc gccatcgtgc atccggcgtt gcaaccgaaa
    17281 taacggagaa catgatggaa gcgatcgatc ccttcgtctt ccgcttttcg attttcatat
    17341 tggcgatttt cgtcgggtat ttcgtggtct ggggcgttac gcccgccctc cacacaccgt
    17401 tgatgagcgt caccaacgcg atctcctcgg tgatcgtggt gggcgccttg atcgcggtat
    17461 cggtgcctgc ggtctccaat gaatcctggc tgtccaaggg gctcggcttc gtcgcgctgg
    17521 tgctcgcctc ggtgaacatt ttcggcggct tcctcgtcac ccagcgcatg ctcgcgatgt
    17581 acaagaaaaa agagaagaag tgaggcggat cgcatgaacg ccaacatcgc cgcactgctc
    17641 tatctcattg ccggcgtgct gttcatcctg gcgctgcggg gcctcgcctc gcccgaaacc
    17701 gcgcgcaacg gaaatcggcg cggcatgatc ggcatggcgc tcgccatcct taccacgctc
    17761 gcggtgacgg gtgtcagcga tgtcttgacg tgggctctgg tccttgcggg gctcggactc
    17821 ggcggcgcca tcggcgcact gacggcacgg cgcatcgcga tgaccgccat gccgcaattg
    17881 gtggcggcgt tccacagcct cgtcggtctc gccgctgtcc ttgtcgcggg agcggcgctc
    17941 tactcaccgg acgcctatgg catctgcgtc cacgacgcgg cgaacatgtg caccggcctc
    18001 atcaagacgc agagcctggt cgagatgagt ctcggcgtcg ccatcggcgc catcaccttt
    18061 gccggatcga tcattgcctt cgccaagctc aacggcaaca tgtcgggcgc gcccatcctg
    18121 ctgcccgcgc ggcatcttct caacctcgtc atcgctttgg ccatcgtcgc gctggtcggc
    18181 tatttcgtca tggtgcaggc gccgtggacc ttctgggcca tcacggtcct ctccttcatc
    18241 ttcggcatca cgctcatcgt tcccatcggc ggcgcggaca tgccggtggt cgtttcgatg
    18301 ctgaactcct attcgggatg ggcggcagcc ggcatcggct tcacgctgga gaacaccgcg
    18361 ctcatcatca ccggcgccct cgtcggttcg tcaggcgcca tcctctccta catcatgtgc
    18421 aaggggatga accgctcctt catctcggtc atcgcgggcg gtttcggcgg agaaaccgct
    18481 gttgccggtg gcgtgaagga aacccgcccc gtgaagcagg gctcggccga agacgcggcc
    18541 ttcatcatga agaacgcggg ctcggtcatc atcgtgccgg gctacggcat ggcggtggcc
    18601 caggcccagc atgcggtgcg cgaaatggcc gacaagctga agcacgaggg cgtgaaagtc
    18661 tcctatgcca ttcatcccgt cgcgggacgg atgccgggcc acatgaacgt gcttttggct
    18721 gaggccaacg tgccttatga tgaagtgttc gaactggaag acatcaactc gcaatttgcg
    18781 caaaccgatg ttgcctatgt gatcggcgcg aacgacgtca ccaatcccgc cgccaagaac
    18841 aatccgcaat cgccgatctt cggtatgccg atcttggatg tggagaaggc caagaccgtg
    18901 ctgttcatca agcgcggcat gggctcgggc tatgccggta tcgagaacga gctgttcttc
    18961 cgcgacaaca ccatgatgct gttcgccgat gcgaagaaga tgacggaaga aatcgtcaaa
    19021 gcgctcggct gacgcgtttc gacatttcgc acg
//
LOCUS       DASYEG010000146         6927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_625972, whole genome shotgun sequence.
ACCESSION   DASYEG010000146 DASYEG010000000
VERSION     DASYEG010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625972"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VGM72_06560"
     CDS             complement(<1..849)
                     /locus_tag="VGM72_06560"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY4264962.1"
                     /translation="MSDLIRRGPRSVFLATENLRGIMLMIVARLVFAGSDTFTKLAST
                     GLPASEVVLLRNVVSLPIVLVMAWRLGGSRHLDAFRDSVVVSRSVLEGTGTLAFVAAL
                     PYVMLGQSTVILLTVPIILVALSALLFREDVGWRRWAAIVVGFAGVIMVAEPFGAHPT
                     YYLLLTQFTAVCWAFRDLMTSRIVGRIPTITVALVNTIIVALLAVPGALWQEWRGFGT
                     QEALYLLGSGGFVAFANFLYIDALRTGAIAVVAPFRYSAAIWASIAGFLVWGDIPDWL
                     GVTGTIF"
     gene            complement(904..1263)
                     /locus_tag="VGM72_06565"
     CDS             complement(904..1263)
                     /locus_tag="VGM72_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264963.1"
                     /translation="MRRFLFAASFLLFADLAMAAAIPSAPIPPPPLPAPQPACVDTRD
                     SADYVPGVDAYGRSVAPADLPGSTTDVQVGTQVYADVRTANPQLRGAGVVVNLPGLQA
                     RPPCPQSAPPKSSVTIH"
     gene            complement(1323..1559)
                     /locus_tag="VGM72_06570"
     CDS             complement(1323..1559)
                     /locus_tag="VGM72_06570"
                     /inference="COORDINATES: protein motif:HMM:NF018978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HEY4264964.1"
                     /translation="MGWALGLALYFIIWWTMLFAVLPFGVHSQAEMKDIVPGTEPGAP
                     ARPRLGLKILVNTGVSAIVWGIADFAYVQFYLPH"
     gene            complement(1572..3155)
                     /locus_tag="VGM72_06575"
     CDS             complement(1572..3155)
                     /locus_tag="VGM72_06575"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEY4264965.1"
                     /translation="MNLNCYGYGPPGARKWIVVDCGVIFGRETTTPGVDLMMPDIRFL
                     EEVREDILGLVLTHAHEDHHGAVAHLWPKLRCPVYATPFTARLLHDKLAEAGIENRVR
                     VKIVPLGGAVTLGPFALEFISITHSIPEPNAIAIRTGLGTILHTGDWKIDPDPLLGEV
                     TNDAALRRVGEEGVLAMVCDSTNALVPGESGSEGEVRRALIRLIGSLKGRIAVTAFAS
                     NVARLQSVAEAAREAGRELVLVGRSMHHMVNCARDTGYLKKFPRVVPEDESELLPADN
                     VLYLCTGSQGEPRAALARIASDNHAHVSLGEGDSVIFSSRIIPGNELAIFELQNQLAA
                     KGVAVITERDHFVHVSGHPARDELAQMYSWVKPKIAVPVHGELRHMSEHARLAASLQV
                     PQAVVALNGQMVRLAPGPAEIVDETPSGRLHLDGRVLVHEDEGFARARRSLGYAGFIG
                     ITLVLDRKGRVACDPALHIEGIPDAVHEPVREAILRAANGKRGRDGDLAEQVRIAARR
                     AANAIWGKKPVVRVQTIEM"
     gene            complement(3221..4006)
                     /locus_tag="VGM72_06580"
     CDS             complement(3221..4006)
                     /locus_tag="VGM72_06580"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009844.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY4264966.1"
                     /translation="MLLAIDAGNTNIVFAVCEGRSIRSQWRAATKTTRTADEYAVWLK
                     EMLALENLKFSDLDAAIVASVIPTALFDLRSLCRRYLKCEPLVIGEPEVALGIGVNVD
                     RPAAVGADRLVNTIAAHDAYPGALIVVDFGTATTFDVVSANGDYEGGVIAPGVNVSAE
                     ALHQAAAMLPRVAIERTQSVIGKDTVPAMQSGLYWGYVGLIEGLIARIKSEYEQPMTV
                     IATGGLAALFHRQVAGIDHLNRDLTIAGLILVHARNAMRPGSP"
     gene            complement(4009..4743)
                     /locus_tag="VGM72_06585"
     CDS             complement(4009..4743)
                     /locus_tag="VGM72_06585"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY4264967.1"
                     /translation="MTEIPGWPERLGLVLHGMLDSTNEEARRLADAGEGGPLWIAARE
                     QTAGRGRRGRIWVSQRGNLFATLLTRAAPSLAAQLAFAAGLAAGETVAAYAPDTEITL
                     KWPNDVLLNGKKTAGILLENLGGDALAIGIGINLEHYPRDTEFPATSIAAATGSTPDF
                     DAALLRLARAMHAWYEVWRDNGFAPIRSAWLARAAGQGCPVIARLEGSEMEGIFETID
                     EDGALLLRQPSGAMTRIASGVVFFRA"
     gene            complement(4740..6164)
                     /gene="nuoN"
                     /locus_tag="VGM72_06590"
     CDS             complement(4740..6164)
                     /gene="nuoN"
                     /locus_tag="VGM72_06590"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEY4264968.1"
                     /translation="MNVALLVPEFIVALGAMALMMIGVFMGDRAQKLLSWLAILLFAA
                     AGVAALMQTGRAQAFNGGFVADGFARFVKLMVLVGAALVLIMGQGYVDRQRMNRFEFP
                     ILVTFSVLGMMLMVSAGDFVTLYLSLELQSLALYVLAAFDRDNLRSTEAGLKYFVLGA
                     LSSGMMLYGISLIYGFAGTISFVKIATVVQTGGVSLGLLFGLVFLISGLAFKISAVPF
                     HMWTPDVYEGAPTPITAFFSASPKIAAMALFVRTMVSAFPDATGEWRQIVIFISILSM
                     ALGAVAAIGQNNIKRLMAYSSIANMGFILVGLATGTAQGVEGVLIYLAIYLVTNIGVF
                     ACILAMRRDGHMVENISDLSGLAQTRPGYAFVFAMLMFSLAGIPPLAGFFAKFYVFLA
                     AIRAGLYPLAVIGVLTSVVGAYYYLRIVKIIYFDEPAPALEREMGASLSTIIAASGIF
                     TVFFLVGAAPLVSAAEAAAASLFP"
     gene            complement(6161..>6927)
                     /locus_tag="VGM72_06595"
     CDS             complement(6161..>6927)
                     /locus_tag="VGM72_06595"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY4264969.1"
                     /translation="LKMGGYGFLRFSLPMFPVASEQFAPVIFVLSLIAIVYTSLVALA
                     QEDMKKLIAYSSVAHMGLVSLGIFTFSHEGIDGGIFQMLSHGIVSGALFLCVGVIYDR
                     MHTRLISAYGGLVNRMPVYAVCFLVFTLANVGLPGTSGFVGEFLTLLAAFRYNSWLAI
                     VGTTGVILSAAYALWLYRRVIYGALEKPALKSISDLSPREFAFMAPLVIITIVLGVYP
                     KPIFDVTRASVAHLVELHHQGVAAAAADRATAEVRP"
BASE COUNT         1510 a         2199 c         2118 g         1100 t
ORIGIN      
        1 gaagatcgtg ccggtcacac cgagccaatc gggaatatcg ccccagacga gaaatccggc
       61 gatgctggcc catatcgcgg cggaatagcg aaacggcgca accactgcga tggcgcctgt
      121 gcgcagcgcg tcgatgtaga ggaaattggc gaacgccacg aagccgcccg agcccagaag
      181 atacagcgct tcctgcgtgc cgaagccgcg ccattcctgc cacaaggcac ccggcaccgc
      241 gagcagcgcc acgatgatgg tgttgacgag ggcgaccgta atggtgggaa tgcgcccaac
      301 gatgcgcgac gtcataagat cgcggaacgc ccagcagacc gctgtgaatt gcgtcagcag
      361 aagataatag gtcggatgcg caccgaacgg ttcggcgacc atgatgacac cggcgaaacc
      421 cacgacaatc gccgcccagc gccgccatcc gacatcctcg cgaaacagca gggcggacag
      481 tgccaccagg atgatgggca ccgtgagcag gatcaccgtg ctctgtccga gcatgacgta
      541 aggcaacgcc gccacgaagg cgagcgtgcc ggtgccctcc agcacgctgc gcgaaaccac
      601 gacggaatcg cggaaagcgt ccagatgccg cgagccgccc agacgccatg ccatgaccag
      661 cacgatggga agtgaaacca cgttgcgcaa caataccact tcgctcgccg gcaaacctgt
      721 gctcgcgagc ttggtgaatg tgtcgctgcc cgcgaacacc agccgcgcga caatcatcag
      781 catgatgccg cgcagatttt cggtggcgag gaacacgctg cgcggaccgc gtctgatgag
      841 atcgctcatg gccgcaaccg gccatggcgc agcttcgcgg tcaagagtgg cgtaaagact
      901 cgatcagtgt atcgtcacag aagacttcgg cggcgctgat tgcggacagg gcgggcgcgc
      961 ctgcaatccg ggcaggttga ccaccacgcc ggcgccgcgc aattgcggat tggctgtgcg
     1021 cacatcggca tagacctgcg tgccgacctg aacatccgtg gtgcttcccg gcagatcggc
     1081 gggcgccacg ctccggccat aggcatcgac gccgggaaca tagtccgcgg aatcgcgtgt
     1141 atcgacgcag gccggttgcg gcgcgggtaa cggaggtgga gggattggcg cggacgggat
     1201 cgcggcagcc atcgcaaggt ctgcgaacag aagaaatgaa gccgcaaaca ggaagcgccg
     1261 catgctggtg ctccgaaagt ctggccacga gcatgatcgg taaagcttaa tatttcctca
     1321 tcctaatgcg gcagataaaa ctggacgtag gcgaaatccg cgatgcccca cacgatggcc
     1381 gagaccccgg tattgaccag gatcttcagc cccagccttg gccgcgccgg cgcgccgggt
     1441 tcggtgccag gcacgatgtc cttcatctcc gcctggctgt gcacgccaaa aggcaacacg
     1501 gcgaacagca tcgtccacca gatgatgaaa taaagcgcaa gacccaatgc ccatcccatg
     1561 acgaagccct atcacatttc tatcgtctgc acgcgcacca cgggcttctt gccccaaatc
     1621 gcgttggcgg cgcgccgcgc ggcgatgcgg acttgctccg cgagatcgcc gtcccggccg
     1681 cgtttgccat tggccgcccg cagaatcgcc tcccggacag gttcatgcac ggcatccgga
     1741 atgccttcga tatggagcgc ggggtcacac gccacccgcc ccttgcggtc gaggaccagc
     1801 gtgatcccga tgaaaccggc atagccgagc gaacggcgcg cgcgcgcaaa gccctcgtct
     1861 tcatgcacca atacgcgccc gtccagatgc aggcggcccg atggcgtctc gtccacaatc
     1921 tccgccggtc ccggggccag ccgcaccatc tgaccgttga gcgccaccac ggcctggggc
     1981 acctgtagcg atgccgcaag ccgcgcgtgc tcgctcatat ggcgcagttc gccatggacc
     2041 ggcacggcga ttttcggctt gacccaggaa tacatctggg cgagctcgtc gcgcgccgga
     2101 tgccccgaca catgcacgaa atgatcgcgt tcggtgatca ccgcgacacc cttggccgcg
     2161 agctggttct gcagctcgaa gatcgcaagc tcgtttcccg gaatgatgcg cgaggagaag
     2221 atcaccgaat cgccttcgcc aaggctgaca tgggcatgat tgtccgacgc gatgcgggcg
     2281 agcgccgcgc gcggctcgcc ctggctgccg gtgcagaggt aaagcacatt gtcggcggga
     2341 agcagttccg attcatcctc cggcacgacg cgtgggaatt tcttgagata gccggtatcg
     2401 cgcgcgcaat tcaccatgtg atgcatggaa cggccgacca gaaccaactc acggcccgcc
     2461 tcgcgcgcgg cttcggccac cgattgcagc cgcgccacat tggaggcgaa cgccgtcacc
     2521 gcgatgcggc ccttgagcga tccgatcaac cggatcaggg cgcggcgcac ttcgccttcc
     2581 gagccggatt cccccggcac tagcgcgttg gtggaatcgc acaccatcgc gagcacgcct
     2641 tcttcgccca cacggcgtag cgcggcatcg ttcgtcacct cgcccagcaa gggatcggga
     2701 tcgatcttcc aatcgccggt atgcagaatc gtgccgaggc cggtgcggat cgcaatcgca
     2761 ttgggttccg gaatggaatg ggtgatgctg atgaattcga gcgcgaacgg tccaagcgtt
     2821 accgcgcccc cgagcggcac gatcttcacg cgcacacggt tttcgatccc ggcctctgcg
     2881 agcttgtcgt gcaagagacg cgcggtgaac ggcgtcgcat aaaccgggca gcgcaatttg
     2941 ggccacagat gcgcgaccgc gccgtggtgg tcctcatgcg catgggtgag cacgaggccc
     3001 agaatatcct cgcgcacctc ttccagaaat cggatgtctg gcatcatcaa atcgacgccg
     3061 ggcgtggtgg tttcacgccc gaatatcaca ccgcaatcca cgacgatcca tttgcgggca
     3121 ccgggcgggc cgtagccgta gcaattcagg ttcatgccga tctcccctgc cccgcccagg
     3181 ggaaggaaaa ccagatcgga atcctcctta ttcaagcgca tcaaggcgaa cccggccgca
     3241 tcgcgttgcg cgcgtggacg agaatgaggc ccgcaatcgt cagatcgcga ttcaaatgat
     3301 cgatgcccgc gacctgccgg tgaaagagcg ccgccaaacc gccggtcgcg atgaccgtca
     3361 tgggctgttc atattcggat ttgatgcgcg cgatgagccc ttcgatcaaa ccgacatatc
     3421 cccagtagag gccggactgc atggccggca cggtgtcctt gccgatcacc gattgcgtgc
     3481 gctcgatagc gacgcggggc agcatggcgg cggcctgatg caaggcttcc gccgacacgt
     3541 tcacgccggg agcgatgacg cccccttcgt agtcgccatt ggcgctcacc acgtcgaagg
     3601 tggtggccgt gccgaaatcc accacgatca gcgcgccggg atatgcgtcg tgggcggcaa
     3661 tggtgttgac cagccggtcg gcgcctaccg ccgccggcct gtcgacatta acgccgatgc
     3721 caagcgcgac ttcgggttcg ccaatcacca gaggctcgca tttgagatag cggcggcaaa
     3781 ggctgcgcag atcgaacaac gccgttggga tgacgctggc gacgatcgcg gcgtcgagat
     3841 cggaaaactt caaattctcg agtgcgagca tttccttgag ccacaccgca tattcatcgg
     3901 cagtgcgcgt ggtcttggtg gccgcacgcc actggctgcg gatcgatcgc ccttcgcaga
     3961 ccgcgaagac gatgttggta ttcccggcat cgatggcgag cagcatggtc aggcccggaa
     4021 gaacactacg ccgctcgcga tgcgggtcat ggcgccggaa ggctgccgca gcagcagcgc
     4081 gccgtcttca tcgatggttt cgaatattcc ttccatctcg cttccctcga gccgcgcgat
     4141 caccggacat ccctgccccg ccgcacgcgc aagccacgcg ctgcgtatcg gcgcgaaacc
     4201 attgtcgcgc cagacctcat accatgcgtg catggcgcgt gcgaggcgga gcaacgcggc
     4261 gtcaaaatcg ggcgtggaac cggtcgcggc ggcaatcgac gtcgcgggga attccgtatc
     4321 gcgcggataa tgttcgagat tgatgccgat accgatggca agcgcgtcgc cgcccagatt
     4381 ttccagcaaa atgccggcag tctttttgcc gttcagcaga acatcgttcg gccatttcag
     4441 agttatttcg gtatccggcg cataggcggc gacagtttcg ccggctgcaa gcccagcggc
     4501 aaaggcgagt tgcgctgcga gcgatggtgc cgcgcgcgtc aacaatgtcg cgaaaagatt
     4561 cccacgctgg gatacccaga tgcgtccccg ccgcccacgg cccgcggtct gttcgcgcgc
     4621 ggcgatccag agcggcccac cctcgcccgc gtccgccaaa cggcgcgctt cttcgttggt
     4681 ggaatcgagc atgccgtgaa gcaccaggcc gagccgttcc ggccagcccg gaatttccgt
     4741 cacggaaaca gcgatgcggc ggcggcctcc gcggccgaca ccaacggtgc cgcgccaacg
     4801 aggaagaaca ccgtaaagat acccgatgcc gcgatgatgg tggaaaggct cgcacccatc
     4861 tcacgctcca gcgccggggc gggttcgtcg aaataaatga tcttcacgat acgcagataa
     4921 taataggcgc caaccacact ggtcaggaca ccgatcaccg ccagcggata aagaccggcg
     4981 cggatcgcgg cgagaaagac gtagaatttc gcgaaaaagc ccgcgagcgg cggaatgccg
     5041 gcgaggctga acatcaacat ggcgaacacg aaagcatagc cggggcgtgt ctgcgccagc
     5101 ccggaaagat cgctgatgtt ctccaccatg tgcccgtcgc ggcgcatggc gagaatgcac
     5161 gcgaaaacgc cgatattggt gaccagatag atcgccagat agatcagcac gccttcgacg
     5221 ccttgcgcgg ttccggtcgc cagcccgaca agaatgaaac ccatattggc gatggagcta
     5281 taggccatca ggcgcttgat attgttctgg ccgatggccg ccaccgcacc gagcgccatc
     5341 gacaggatgg aaatgaagat gacgatctgg cgccactccc cggtcgcatc cgggaaggca
     5401 ctgaccattg tccgtacaaa caacgccatg gccgcgatct tgggcgatgc ggagaaaaag
     5461 gcggtgatgg gcgtgggcgc gccttcatag acgtccggcg tccacatgtg gaacggcacc
     5521 gcggagatct tgaaggccag tcccgaaatc aggaagacga gtccgaacag gagaccgaga
     5581 gataccccgc ctgtctgcac gaccgtcgcg atcttgacga aagaaatggt gcccgcgaaa
     5641 ccgtaaatca gcgagatgcc ataaagcatc atgccggagg agagcgcacc caggacgaaa
     5701 tatttgaggc ccgcttcggt ggatctgaga ttatcgcggt cgaaggccgc gagcacataa
     5761 agcgccaggc tctggagttc gagactgaga taaagcgtaa cgaaatcccc cgccgagacc
     5821 atcagcatca tgccaagcac ggagaatgtc accaggatgg ggaactcgaa ccggttcatc
     5881 ctctgccggt cgacatagcc ctgtcccatg ataaggacga gcgccgcgcc aacgagcacc
     5941 atcagcttca caaaacgcgc gaacccgtcg gcaacgaagc cgccgttgaa cgcttgcgcg
     6001 cggcccgttt gcatcaatgc ggcgacaccg gcggccgcga acaaaaggat cgccagccac
     6061 gacagcaatt tttgcgcgcg gtcgcccatg aagacgccga tcatcatcag tgccatggcg
     6121 cccagcgcca caatgaattc gggaaccaga agtgccacgt tcatggccgc acctccgctg
     6181 ttgcgcgatc ggctgcggcg gcggcaacgc cctgatgatg caattcgacg agatgggcca
     6241 cggaagcgcg cgtcacatcg aatatcggtt tgggataaac gccaagcaca atggtgatga
     6301 tcaccagtgg tgccatgaag gcaaactccc gcggcgaaag atcggagatg gatttgagcg
     6361 ccggtttttc cagcgcccca tagatgacgc gccgatagag ccacagcgca taggccgccg
     6421 acaggatcac cccggtggtg ccgacaatcg ccagccagct attatagcgg aaggccgcca
     6481 gcaatgtcag gaactcgccg acaaatccgc tggtaccggg caagcctaca ttggccagcg
     6541 tgaacaccag gaagcacacg gcgtagacgg gcatgcggtt gacgagcccg ccataggcgg
     6601 aaatcagccg cgtatgcata cggtcgtaga ttacgccgac gcaaaggaag agcgcgcccg
     6661 agacgatgcc atggctcagc atctggaaaa taccgccatc gatgccttca tggctgaagg
     6721 tgaagatgcc gagcgagacg agccccatat gcgccacgga agaataggcg atcagcttct
     6781 tcatgtcctc ctgcgccaac gccaccagcg aggtgtagac gatggcgatc agcgacagca
     6841 caaagatcac cggcgcgaat tgctcgctcg ccaccgggaa catcggcagc gagaaccgca
     6901 agaaaccgta tccgcccatc ttcaaaa
//
LOCUS       DASYEG010000147         3307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3525301, whole genome shotgun sequence.
ACCESSION   DASYEG010000147 DASYEG010000000
VERSION     DASYEG010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3525301"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="VGM72_06600"
     CDS             <1..987
                     /locus_tag="VGM72_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264970.1"
                     /translation="LNAWPRRGGRGVYINHDASRTSNDCYVCEIPPGKSLTPQRQLFE
                     EMVLVLDGRGSTSVWDDCGHRITFEWKAGAMFAIPLNCWHQHFNGSGQEAVRFVAVTN
                     GPAVMNLYDDADFVFNTAYDFKNRFAGEPDYFSPKTEPKGFLLPTNFVPDAVNLPLIS
                     AKERGAGGGHIRFNMAKGSMSSHISQFPVGTYKKAHAHGPSAHVIILSGQGYSLMWAE
                     GEEPKRYDWEVGTLIVPPNKFFHQHFNSGPVPARYLAFKHEVALIRNSQGVPTAWISR
                     RVGGDQIDYSDETPRIRSMFHEALATHGITPKMDDAYQKELADLPPQKVAAE"
     gene            1010..2050
                     /locus_tag="VGM72_06605"
     CDS             1010..2050
                     /locus_tag="VGM72_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY4264971.1"
                     /translation="MERRTFVTAAAMVAMFAPWKACAQTGVNPAHLRGPDRTARLIAG
                     AKREGFLNLYSSAVTEHMNAVMSAFEKKYGVKVRLWRGGSEEILQRAVTEARGGRFDA
                     DVMETASTQMIALAREKLLQPVDSPIAGDLMREAKIPGQPFLPSRLIVFTGAYNTNLI
                     RPDDLPKRYEDLANPKWKGKLGIEADDNNWLMALAGAYGEDKAAGLLRDIVTKNGISV
                     RKGHTLMANLVASGDVPMALTIYYHEVEPLKRAGAPIQELNIAPVFAFTSSVGVASHA
                     PHPNAAVLFQDFLLSDGQAILAAHDNLPANVTFQRLPKNIAFSFFDVPKYMAESAKWT
                     KLYKDILARQTR"
     gene            complement(2546..2620)
                     /locus_tag="VGM72_06610"
     tRNA            complement(2546..2620)
                     /locus_tag="VGM72_06610"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2586..2588),aa:Val,seq:cac)
     gene            complement(2688..>3307)
                     /locus_tag="VGM72_06615"
     CDS             complement(2688..>3307)
                     /locus_tag="VGM72_06615"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4264972.1"
                     /translation="PELLLLDEPLSNLDAKLRERMRFELKRIQRELGLTTVYVTHDQA
                     EALALSHEIAVMNEGRIVQIGNPRTIYERPLNRFVAEFIGSSNFLDGVVLGPDNRPGF
                     WRVRGACGPLLAFSETPLQPGGQVTLSVRPENIELSQTVFESGVNRLIGIVDSKVFQG
                     DFADFQVRVDGLTLLTRTHPSLRTPVGENIHIRIDPEKCVVLAGG"
BASE COUNT          703 a          994 c          971 g          639 t
ORIGIN      
        1 ctgaatgcct ggccgcggcg tggcggccgt ggcgtctaca tcaatcacga tgcctcgcgc
       61 acctcgaacg attgttatgt ctgtgaaatc ccccccggca agagtctcac gccgcagcgc
      121 caactcttcg aggagatggt gctggtgctc gatgggcgcg gctcgacatc ggtgtgggac
      181 gattgcgggc atcgcatcac ctttgagtgg aaggccggcg ccatgttcgc gatccccctg
      241 aattgctggc accagcattt caacggttcg ggtcaggaag cggtacgttt tgtggcggtc
      301 actaatggtc ccgccgtgat gaatctctat gacgatgcgg atttcgtctt caacacggct
      361 tatgacttca agaaccggtt tgccggagag cccgattatt tcagtcccaa gacggagccc
      421 aagggcttcc tgctgcccac caatttcgtg ccggacgcgg tgaacctgcc gctgatttcc
      481 gcgaaagagc gtggcgcggg cggcggccac atccgcttca atatggccaa gggatccatg
      541 tcgagccaca tctcgcaatt tcccgtcggc acctacaaga aggctcatgc gcacggtccg
      601 agcgcgcacg tcatcattct ctccggccag ggctactcac tgatgtgggc cgaaggtgaa
      661 gaacccaaac gctatgattg ggaagtcggg acactgatcg taccgccgaa caaattcttc
      721 caccagcatt tcaattccgg tccggtcccg gctcgctatc tcgccttcaa acacgaggtg
      781 gcgttgatcc gcaattctca aggcgtcccg acggcctgga tcagccgccg cgtgggtggc
      841 gatcaaatcg attattccga cgagacgccg cggatccgct ccatgttcca cgaggcgctc
      901 gcaacgcacg gcataacccc gaaaatggat gacgcctacc agaaggaatt ggccgacctg
      961 ccgccccaga aagtcgcagc ggagtgagca caagacattg gccagcaaaa tggaacggcg
     1021 caccttcgta acggccgcgg caatggttgc catgttcgcg ccatggaaag cgtgcgcgca
     1081 gaccggcgtc aatcccgccc acctgagggg tccagatcgt acggcgcggc tgatcgcggg
     1141 cgccaagagg gaaggcttcc tcaacctcta ttcgtctgcc gtcaccgagc acatgaatgc
     1201 ggtgatgagc gcgttcgaaa agaaatatgg tgtcaaggtt cgcctctggc gcggcggctc
     1261 ggaggaaatc ctacagcgtg ccgtgaccga agcacgcggc ggacgcttcg atgccgatgt
     1321 catggagacg gcttcaaccc agatgatcgc gctcgcgcgc gagaagctgc tgcagcccgt
     1381 ggactctcca atagcaggcg atctgatgcg cgaagcgaag ataccggggc agccctttct
     1441 gccgtcgcgc ctcatcgtct tcaccggcgc ctacaacacc aatctgatca ggccggacga
     1501 tctgccgaaa cgctacgagg atttggccaa tccgaaatgg aagggcaaac tcggcatcga
     1561 agcggacgac aacaattggc tgatggcgct tgcgggcgcc tatggcgagg acaaggccgc
     1621 cgggctgctc cgcgatatcg tgacgaagaa cgggatttcg gtgcgcaagg gccacacgct
     1681 gatggccaat ctcgtggcgt ccggcgatgt gccgatggcg ctgacgatct attatcatga
     1741 ggtggaaccg ctgaagcgcg cgggcgcgcc aattcaggag ttgaatatcg ccccggtgtt
     1801 cgcgttcacc tccagcgtgg gcgtggcgtc gcacgcgccc catcccaatg ccgcagtcct
     1861 gttccaggat ttcctcttgt cggacggaca ggcgattctc gccgctcacg acaatcttcc
     1921 ggccaatgtg acgtttcaac ggctgccgaa gaacatcgcg ttttcgttct tcgatgtgcc
     1981 gaaatacatg gccgaaagcg cgaagtggac gaagctgtac aaggatatcc tggcacggca
     2041 aacgcgttaa tgttcggcac gcgcatgtgc cgtccacagc gcaggtaacc atatcgcgcg
     2101 gaatccattc acgcctgaaa aagtaagcgc tacaaattcg agctcgatgc ttgtgacgca
     2161 tatgtggagc gcaaatggcg ttaatgtacg atccaatcgc gcgagatcaa ctgaacgaaa
     2221 tgcgcgtcaa cttgggcacg ttctacgtca gtgcgatttg ggcgcagcgt gaatacgagc
     2281 aaaaagcgcg caacgcagag gccctggaac tatggcggca gtcgtttccg gcatgggacc
     2341 ggctatttgg caggcctacg cccggtgttc gaaccgatag cgcgcacatc atcgcgatgc
     2401 ctgcccgaac gatgccagac caacaacgct gcgcgtaact acctcagttc ttgaagtcgg
     2461 ccacagtcca ccgcacaccc gctctcgttc agacataacg ggaatattag gtccctcggt
     2521 aatcgccggg tgagcttaat aattgtggtg ggcgcggcat ggattgaaca tgcgacccct
     2581 cccgtgtgaa gggagtgctc tcccgctgag ctacgcgccc ctttcgggga ccacgcctat
     2641 aggattgggt cgcggctcaa gtcaagcggc ggtgacattt tttccgctta accgcctgcc
     2701 aggaccacgc atttttccgg atcgatccgg atgtggatat tctcgcccac gggtgtgcgc
     2761 agggaaggat gcgtgcgtgt gagcagcgtt aggccgtcca cccgaacctg gaaatcggca
     2821 aagtcaccct ggaacacctt gctgtctaca atgccgatga gacgattaac cccgctttcg
     2881 aaaacggtct gcgacagctc gatattttcc ggccggaccg aaagcgtaac ctgacctccg
     2941 ggctgaagcg gtgtttcgct aaacgccaga agcggcccgc acgcgccacg cacgcgccag
     3001 aagcccggcc gattgtctgg gcccaacacc accccgtcga gaaaattact actgccgatg
     3061 aattcggcga cgaagcggtt gagagggcgt tcatagatcg tccgaggatt gccgatctgc
     3121 acgatgcgcc cttcgttcat caccgctatc tcatggctga gcgccaaagc ctcggcctgg
     3181 tcgtgcgtga catagaccgt cgtcagaccc agctcacgct ggattcgctt caactcgaac
     3241 cgcatgcgct cgcgcaactt cgcatcgaga ttggagagag gctcgtcgag caggagcagt
     3301 tcaggct
//
LOCUS       DASYEG010000148         5315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2654991, whole genome shotgun sequence.
ACCESSION   DASYEG010000148 DASYEG010000000
VERSION     DASYEG010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2654991"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..2108
                     /locus_tag="VGM72_06620"
     CDS             150..2108
                     /locus_tag="VGM72_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264973.1"
                     /translation="MARNLSGRLKFFAWGTAAALALALSDGPGLAQDRGAPMPEATGA
                     PRNILPVEQMPAQQTPEAPSPPAMPDGVQMPARIPQRGAAVQIGDLGTLEGPIAGTLD
                     NTNGGLGPTAWQASERDTIVTMLQAVPAATPSAAQRLLMRKVLLTAAVPPPGRAMVSF
                     NQLRLTKLLEGGYVADAADLARSIRAPMNFDILRSQTDAMLYAGRDMDACSDITAHRL
                     DSADSFWVELRAFCYAVSGDTGPLDLTRAVIASQGLADPAFVTLLDGMTSGKPTAPSI
                     VRYPDSIHIVMMARLNLPLTTDTATGLGLPASLLTTASTASPRPLRVAAAEKALRAGV
                     LPIPLLAEILDTSAFTPQDLDGAPALARAEPLMDGLARIRAALKTAGNNEMRAELVHT
                     ALEIGEREGLLAQVAALYADEAAQIIPAPDWSNWSDLMVRGLLLADRAEAAQRWFDIL
                     DRNMPGMADTVDQLDLAMAMAAPNARRNAEARQLLEALALAVHPPPDPVVVEMPAAAP
                     DPGVGDPLTAAPPPPMPPAPRPKPPRALLTRATLDLGLFDALGELNSPEAQGAVQPLV
                     SEPSPGRRPPPVLMQRIDKAALSDARGEVALSVATVLGPQGARDLAPDIVVRLVRALQ
                     TSGIHDGAHALAMEALLLRPRAGGSGTP"
     gene            2105..3088
                     /locus_tag="VGM72_06625"
     CDS             2105..3088
                     /locus_tag="VGM72_06625"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEY4264974.1"
                     /translation="MTGKKTPRGSEQDRTLVESFLDMMSAERGAARNTLDAYARDILA
                     FAQDCAKAGCDLKTAGRDRIRAHLANLSAASIKPSSQARKLSALRRFYGFLYSEGLRR
                     DDPCGAVEAPRLSRPLPKILSAAEALKLMEAAREGEETSPERARLLLIVEMLYASGLR
                     ISELVALPLSSVRARERFIHVRGKGGRERLAPVGAAARAALDAYLPMRDSFLPRGKPG
                     LAASRYLFPSRGAEFHLTRRRCHQMLKTLAVKAGIDPGRLSPHVLRHAFATHLVEGGA
                     DLRSVQTLLGHADIATTQIYTHVARDRLRRTMETAHPLARAKPRVGAAEKP"
     gene            3161..4144
                     /locus_tag="VGM72_06630"
     CDS             3161..4144
                     /locus_tag="VGM72_06630"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEY4264975.1"
                     /translation="MNIFLDFEKSIADLEGKIEELRQMGSADGVDDGARNAGVNIDEE
                     IARLQMKTAQLVAETYARLTPWQKTQVARHPARPHFSDYASHLLTDFTPLAGDRLYGE
                     DKAIVAGLGRLRGRAVAVVGHEKGNDTKSRLTHNFGMALPEGYRKVQRLFDMANRFKL
                     PVISLIDTAGAYPGIGAEERGQAEAIARSTQACLGLNTPFVATIIGEGGSGGAVAIAA
                     ANKVYMLEHAIYSVISPEGCASILWHKSDKAADAAAAMKITAQDLLGLKVIDGIITEP
                     VGGAHRAPERAIQAAGDMIEHALQELSPLPAEELKRLRREKYLQIGRLETA"
     gene            complement(4200..5102)
                     /locus_tag="VGM72_06635"
     CDS             complement(4200..5102)
                     /locus_tag="VGM72_06635"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEY4264976.1"
                     /translation="MAANAASAASAQEERQKKQAEIPRCAQPLGNLAVEEPQRNWWSE
                     LKLGSPEALIKVFVQQSGCFSMVDRGAGLAAAQRERALASGGGLQQGSNVGGGQIVAA
                     DYVLVPDIISQNGRASGNSLGGALGGLLGGPVLGAIAGGISFNSSTADVTLAITNVRT
                     TQVMATIDGHSKKTDIGFGVGGGVFGYGGFGAAGATGYNNTEIGQVITLAYLDAYTKL
                     VDQLGGLPGPGQAPGAAAQQMVVATRGTSMFSQASTQSQVIRPVDQGMRLYPTGNKNG
                     LMWEVKDELGNQGWVSSIMFELAK"
BASE COUNT          941 a         1688 c         1710 g          976 t
ORIGIN      
        1 ttgttgaaag atgggttgga aattgctgat cgctttcgtg ttagtgttgg tggtcggtgc
       61 gggggctctg gcgttttatg gggcgagcgt tggacccacc cagcatccca tcgaacaggt
      121 tctgcccgac aaccggtttc caaagtgaga tggcacgaaa tctctcaggc cgactgaaat
      181 tcttcgcgtg gggcacagcg gccgcactgg ctctcgctct ttccgatggc cccggtctgg
      241 ctcaagatcg gggtgcgccg atgccggagg ccaccggcgc accgcggaac attcttccgg
      301 ttgagcagat gccggcgcag cagacgcccg aggcgccgtc gccgccagcg atgcctgacg
      361 gcgttcagat gccagcgcgc attccccagc gcggcgcggc ggtgcaaatc ggcgacctcg
      421 gcacattgga aggccccatc gcgggcacgc tggacaacac caatggcggc ctcggcccca
      481 cggcgtggca ggccagcgag cgcgacacca tcgtgaccat gctgcaggcg gttcccgcgg
      541 ccacgccatc ggccgcgcaa cggcttttga tgcgcaaagt gctgctcacg gcggcggtgc
      601 cgcccccggg acgcgcgatg gtttcgttca atcagctccg tctgacgaaa ttgctggaag
      661 gcggctatgt cgccgatgcg gccgatctcg cgcgcagcat ccgggcgccg atgaatttcg
      721 acattttgcg cagccaaacc gatgcgatgc tgtatgccgg gcgagacatg gatgcgtgca
      781 gcgatatcac tgcgcatcgc ctggacagcg cggattcgtt ttgggtggag ttgcgggcgt
      841 tctgctatgc ggtctccggc gataccgggc cgctcgacct tacccgcgcg gtcattgcct
      901 ctcaggggct tgccgatccc gcgttcgtga ccttgctcga tgggatgacc agtggaaagc
      961 cgacggcgcc aagcatcgtg cgctatcccg attcgattca catcgtcatg atggcgcggc
     1021 tcaatctgcc gctgacaacg gacacggcca ccggactcgg actcccggca agcctgttga
     1081 cgacagcctc gaccgcatcc ccccggccgt tgcgggtggc ggccgcggag aaagcgctac
     1141 gtgccggcgt cttgcccatc ccgctgctgg ccgagattct cgatacctcc gccttcacgc
     1201 cgcaggacct cgatggtgcg cccgctttgg cgcgtgcgga gccgttgatg gatggcctcg
     1261 cgcgaatccg tgctgcgctg aagaccgcgg gcaacaacga aatgcgtgcc gagctggtcc
     1321 acacggccct ggagatcggc gagcgcgagg gcctgctggc acaggttgcc gctttgtatg
     1381 cggatgaggc cgcgcagatc ataccggcac cggattggag caattggtcg gatctgatgg
     1441 tgcgcggact tctgctcgca gatcgcgcgg aggccgcgca gcgctggttt gatatcctcg
     1501 accgcaatat gccgggcatg gccgataccg tcgatcagct ggacctcgcg atggcaatgg
     1561 cggcgccaaa cgcacgccgc aacgcggagg cgcgccagct cctggaggcc ttggctctgg
     1621 ccgtgcatcc gcctccggac ccggtggttg tggagatgcc cgcagccgcg cccgatcccg
     1681 gcgtgggcga tcccctgacg gccgcgccac cgccgccgat gcctccagcg ccgcgtccca
     1741 agccgccacg ggctttgctg acgcgcgcca cgctggacct tgggcttttc gatgcgctcg
     1801 gcgaattgaa ctcgcccgaa gctcagggag ccgtgcagcc gcttgtatcc gaaccatccc
     1861 ccggccggcg tcctcccccc gtcttgatgc agcgtatcga caaggccgcg ctgtcggacg
     1921 cgcgcggcga ggtggcattg tcggtcgcca cggttctggg tccgcaaggc gcgcgcgatc
     1981 ttgcccctga tatcgtggtg cggctggtgc gcgccctaca gacctcgggc attcacgatg
     2041 gggcccatgc gctcgccatg gaagcgttgt tgctgcgtcc gcgcgcgggc gggtccggaa
     2101 cgccatgacg ggcaagaaaa cgccgcgcgg gtccgagcag gaccgcacgc tggtggaatc
     2161 tttcctggac atgatgagcg ccgaacgcgg cgcggcgcgg aacacgctcg atgcttatgc
     2221 gcgtgacatt ctggctttcg cgcaagattg cgccaaggcg ggttgcgatc tcaaaaccgc
     2281 cggccgcgat cggattcggg cgcatctcgc caatctgtcg gccgcctcga tcaagccatc
     2341 ctcgcaggcc cgcaaattgt cggcgcttcg ccgcttctat ggttttctct atagcgaggg
     2401 cctccggcgc gacgatccgt gcggagccgt ggaagcgccg cgcctgtcgc ggccgctccc
     2461 gaaaatcctg tccgctgccg aggcccttaa actcatggag gccgcgcgcg agggcgagga
     2521 gacaagtccc gaacgcgcgc gcctgctcct gattgtggaa atgctctacg cctcgggact
     2581 gcgtatcagc gaactcgtcg cgttgccgct ttcatcggtg agagcgcgcg aacgcttcat
     2641 ccatgtgcgc ggcaaaggcg ggcgcgaacg gctggcgccc gttggcgccg cggcgcgcgc
     2701 ggcattggac gcctatcttc cgatgcgtga ttcgtttctg ccgcgcggca agccgggact
     2761 tgccgcatcg cgttatctgt ttccgtcacg cggcgcggaa tttcacctga cgcggcggcg
     2821 ttgtcaccag atgttgaaga ccctcgcggt caaagcggga atcgatcccg gccgcctgtc
     2881 gccgcatgtc ctgcggcatg cttttgcgac tcatctggtc gaaggcgggg ccgatctcag
     2941 aagcgtgcag accctgcttg gccacgccga tattgccacg acccagatct atacccatgt
     3001 agcgcgcgac cgtctcaggc ggaccatgga aacggcccat ccgcttgcgc gggcgaagcc
     3061 tcgcgtgggg gcggctgaaa aaccgtaggc ggatgatttg acagtccccc attccgcttg
     3121 ccactctcga aatgatttca aatgtacatt atgagcccgc atgaacattt tcctggattt
     3181 tgaaaaatcc attgccgacc tcgaaggcaa aatcgaggaa ttgcggcaga tgggctcggc
     3241 ggatggcgtc gatgacggcg cgcgcaacgc cggcgtcaat atcgacgagg aaatcgcgcg
     3301 cttgcagatg aagacggcgc aattggtggc cgaaacctat gcccggctca ccccctggca
     3361 gaagacgcaa gttgcccgcc atcccgcgcg gccgcatttt tccgactatg cgtcgcatct
     3421 gctgaccgac ttcaccccgc tcgcgggcga ccggctctat ggcgaggaca aggccatcgt
     3481 cgcggggttg ggaaggttgc gcggccgggc ggtcgccgtt gtcggccacg agaagggcaa
     3541 cgacaccaaa tcgcggctga cgcacaattt cggcatggcg ctgccggagg gttaccgcaa
     3601 ggtgcagcgt ctgttcgaca tggccaatcg cttcaaattg cccgtgattt cgctgatcga
     3661 caccgcgggc gcttatcccg gtatcggcgc ggaggaacgc ggacaggccg aagcgattgc
     3721 acgttcgacc caagcttgcc tcggattgaa tacgcctttc gttgcgacca tcatcggcga
     3781 gggtggatcg ggcggagcgg tggccatcgc tgcggccaac aaggtctaca tgctcgaaca
     3841 tgcgatctac tcggtgatct cgcccgaagg ttgcgcatcg atcctgtggc ataaatccga
     3901 taaggccgcg gacgcggcgg cggcgatgaa gatcactgcg caggacctgc tgggcctgaa
     3961 agtgatcgat ggcatcatca ccgaacctgt cggcggagcc catcgtgcgc ccgagcgggc
     4021 gatccaggcg gccggcgata tgatcgagca cgcattgcag gaattgtcgc cattgcccgc
     4081 cgaggagttg aagcgtctgc ggcgcgagaa atatctgcag atcgggcggc ttgaaacggc
     4141 gtaaaaagca aaaacgcccg cagccgggcc acgggcgttt ttggaaaatc gcggcgagcc
     4201 tatttcgcca gttcgaacat gatcgacgag acccagccct gattgccgag ctcatccttc
     4261 acttcccaca tcaggccatt cttgttgccg gtgggataga gccgcattcc ctgatcgacg
     4321 gggcggatga cctgggattg cgtcgaggct tgcgaaaaca tcgaggttcc gcgcgtggcg
     4381 acaaccatct gctgcgccgc cgccccgggc gcctggccgg gacccggtag cccaccgagc
     4441 tgatcgacca atttggtata ggcatcgaga taggcaagcg tgatgacctg gccgatctcg
     4501 gtgttgttgt aacccgtggc gccggccgcg ccgaaaccac catagccgaa gacaccgccg
     4561 ccgacgccga agccgatatc ggtcttcttg ctgtgaccgt cgatcgtggc catcacctgg
     4621 gtcgtgcgca cattggtgat ggcaagcgtc acgtcagcgg tcgatgagtt gaagctgatg
     4681 ccgccggcga ttgcgccaag caccgggccg ccaagcaagc cgcccaatgc cccccccaaa
     4741 ctattgccgc tggcccttcc gttctgagag atgatgtcag ggaccaggac ataatcggcg
     4801 gcgacgatct gaccgccgcc gacattggag ccttgttgca gaccgccgcc cgaggccagg
     4861 gcgcgctcgc gctgcgccgc cgccaaacct gcgccccggt ccaccatcga gaagcatccc
     4921 gattgctgca cgaacacctt gatcagggct tccggcgaac cgagcttgag ttcggaccac
     4981 cagttgcgtt gcggttcttc gacggcgaga ttgccaaggg gctgagcgca gcgggggatt
     5041 tccgcctgct tcttctgccg ttcctcctga gccgaagccg cagaggcggc gtttgcggcc
     5101 atgcaaagtg cagcgccggc caagagcgcg cacgataatg attttgcgga catgggaccc
     5161 ccttagcgaa aatggaaatc aaatctatca gcaaatttgg gatttggcac gcgccgtcgc
     5221 gagacccaaa actcagacac tttcatccat ttggatgaaa gtgtctggcc ggattgagtt
     5281 ttttgaaatg agagcgcgtg tatttcgaag gtgcg
//
LOCUS       DASYEG010000149        29553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4686032, whole genome shotgun sequence.
ACCESSION   DASYEG010000149 DASYEG010000000
VERSION     DASYEG010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 29553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29553
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4686032"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2159)
                     /gene="uvrB"
                     /locus_tag="VGM72_06640"
     CDS             complement(<1..2159)
                     /gene="uvrB"
                     /locus_tag="VGM72_06640"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEY4264977.1"
                     /translation="MAHNNGPAFRPVSGLAEAPAAFRHMTGAEMTAFVPHRPVRPEKS
                     EGGKRFKLVSDYQPAGDQPEAIRELAEGLCGQGPSGTPERDQVLLGVTGSGKTFTMAK
                     IVERLQRPALVLAPNKTLAAQLYSEMKGFFPENAVEYFVSYYDYYQPEAYIPRTDTYI
                     EKDSSVNEQIDRMRHAATRSLLERDDVIIVASVSCIYGIGAVETYSGMAFSITRGQQV
                     SRGDLLARLVALQFRRNDEAFTRGAFRVRGDTVDIFPAHYEDRAWRVTLFGDEVDSIY
                     EFDSLTGQKSAEMETVKIYANSHYVTPRPTLAQATKGMREELRLRLDQFRREGKLLEA
                     QRLEQRTMFDLEMIEATGSCAGIENYSRWLTGRRPGEPPPTLFEYLPDEAIVFVDESH
                     VTVPQLGAMYKGDFRRKATLAEYGFRLPSCIDNRPLKFEEWDAMRPQTVSVSATPGPW
                     ELGRTGGVFTEQVIRPTGLIDPPVEIRPVEHQVDDLLAECKLIAKAGYRALVTTLTKR
                     MAEDLTEYLHENGVRVRYMHSDVETLERIEIVRDLRLGAFDILVGINLLREGLDIPEC
                     ALVAILDADKEGFLRSETSLVQTIGRAARNVDGKVILYADHITGSMERAMAETSRRRA
                     RQEAYNLAHGITPLSIKKSITDIMGSAYERDHVLVDSGLADDPVPIGHNLRSHIAELE
                     KRMRAAAGDLEFEEAARLRDEIKRLQVTELAIADDPYA"
     gene            complement(2264..2650)
                     /locus_tag="VGM72_06645"
     CDS             complement(2264..2650)
                     /locus_tag="VGM72_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264978.1"
                     /translation="MKISRAIFCTFAIGSMTALAPVAMADGPQAINTAAAHAGLAAAA
                     GGIDMVHTHLHHVLNCLVGPGGDGFDAAPGNPCANAGGGAIPQTADAATKAKLQTAAT
                     EAKAGIANNDMAGAKKTAGDIQAMLK"
     gene            complement(2725..4263)
                     /locus_tag="VGM72_06650"
     CDS             complement(2725..4263)
                     /locus_tag="VGM72_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEY4264979.1"
                     /translation="MSSIADPSVDEAAIGAINDAAGYCRTLWATFIGYAATIFVLTSG
                     TTHEALLRRTPVKLPLFNVDSGIPLLLFYVIAPLLLVLFHLNLLMKLHDLRVRVSELI
                     LPLNYCDDANAQFERNRLRRRLMAFDYAMLKAGLVTDRRERWLLALVSDSTIFFMPVL
                     LLLFVQLQFLPYHDGKITWLQRGFVIADLGLIAYIQLWLRAKRAPAATQAAGAMAGWT
                     SRLRLHRMAMSALFVLPLLVSTIVLAFPGEPQDVFWRTLWSMTHSQTQAFWPLAGTDF
                     AEKFGIRRSLNLADRTLWAELPSEELLQALNQDTIVIALRDDIEGVSPEMQRTVERIR
                     NAALSLRGRNLSYANFENATLIAADFRPDLSNNQLRDLSVARTAGEDSNELGMLPAST
                     DITGASFSGANLRGARFPYVLADDAEFSAANLQSAVLAFAQLRNADFYQARLQNASLE
                     SAQLQGATMAEAQLQSTDLKHAHVTIDQLMVAHWEDAELDFDIAEQLKTSFPRTGDHE
                     GQPK"
     gene            complement(4326..5078)
                     /locus_tag="VGM72_06655"
     CDS             complement(4326..5078)
                     /locus_tag="VGM72_06655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HEY4264980.1"
                     /translation="MVDWVAKLKRELTRPFRRNLPPSIPLDFSPAQKTLLKQVRPYTM
                     TSNERVAVLESAVRHVVAQNYPGAFVECGVAKGGSTMAMAYTLLELGIRDRELYLYDT
                     FEGMPEPEDVDRGRFGEAASRSWRKRRDELGRSTWINHGLDDVRANLTLTGYPPDRLH
                     FIKGKVEDTLPAAAPPGAIALLRLDTDWYASTKAELDHLYPKLVRGGIVIIDDYFRWQ
                     GARKAVDEYGAAQRIPIFWVRVDDSSVIGVKP"
     gene            5201..5638
                     /locus_tag="VGM72_06660"
     CDS             5201..5638
                     /locus_tag="VGM72_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264981.1"
                     /translation="MSEIPPANPPNPAAPHYDAAGGASDLRSLAIVCYVLFLLASVNG
                     LSAIVGVIIAYVKRRDAAGTLWQSHFANMILVFWVTVGAAILGFLTWPIALGAFFVAW
                     PVFWPPVLTLPFAFGFLFFPLLVLWYLYRIVRGLIRASEERTY"
     gene            complement(5718..6479)
                     /locus_tag="VGM72_06665"
     CDS             complement(5718..6479)
                     /locus_tag="VGM72_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEY4264982.1"
                     /translation="MKKTMQIALGALGLASASAWTSPAFAIDGLTANAAITTDYIFRG
                     ITQSAKRPAVQGGLDYAVPDTGFAVGTWVSSINFGDDSNLEWDIYGNYNFNLGPVGAS
                     VGVYGYVYPYSGNGGPYDVFELDAGLSHDFGVLAWSAKAFYAPSLPSGYLTVRNGYNP
                     DSEYFLTTGVSVPIAPWLSLSGNVGYEGYSGGKPAGVSDDSYVEWDVGVTLTYDKYSL
                     DLRYLDTDTRTFSSYTAPYFATGPFYVATFSFKFP"
     gene            6747..7529
                     /locus_tag="VGM72_06670"
     CDS             6747..7529
                     /locus_tag="VGM72_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY4264983.1"
                     /translation="MLGTKPSQPGGRELLEILFPGRWPPIWFAHDEGRANKDAFRGHT
                     AIGIGRPMTLTLENHPRAARIFAGKPARLILVRHGKPAIPTNPRTSHHGFRRYIDAYQ
                     DAGLDPQSAPPEELLDLVKGLNAVFTSNLPRANDSARTLLPEAEIIADPLFTEAPLAV
                     PRIPLLKMKVPIWAVMARIMWHAGYHPEIENYRRAKTRASQAADILLGRAEANDGVAV
                     LVAHGYFNAMIGRVLKKRGFSRTGSHRIRFWNAVIYERASSS"
     gene            complement(7542..8135)
                     /locus_tag="VGM72_06675"
     CDS             complement(7542..8135)
                     /locus_tag="VGM72_06675"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEY4264984.1"
                     /translation="MSEAPSALNIPVPQTLDLLLSRRSGSAKAMKGPGPDSAQLCRIL
                     TAGVRVPDHGKLTPWRFILFEGDGRVRMGDILAETIAGERDSSPERVEQERRRFLRAP
                     VIVAVVSRTREQIPIPIWEQELSAGAVCMNIVIAAHAMGFLANWITEWCAYHPKVLER
                     IGLKPGERIAGYIYIGHPVDTLEDRPRPPVDSMTTKF"
     gene            8303..9136
                     /gene="cysE"
                     /locus_tag="VGM72_06680"
     CDS             8303..9136
                     /gene="cysE"
                     /locus_tag="VGM72_06680"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF041874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEY4264985.1"
                     /translation="MTRDRNMRAAEKVASCDPIWSALREQAEQLALSEPELASFAHAT
                     ILKHDRLEAALSYHLAKKIGGEDLSPMQVRETFEEAFAADPAIGQAVRADLSAVFQRD
                     PACHSYIQAFLFFKGFHSLESHRIAHWLWRSGRSWMAYFFQNRVSDLFAVDIHPAAPF
                     GRGIFIDHATGLVVGETAMVEDDVSMLHGVLLGGTGKESEDRHPKVRRGVLLGAGAKV
                     LGNIEVGEYSRVGSGSVVLKSVPPRTTVAGVPARVIGKAGSDRPSQDMDHCLPEPDLG
                     T"
     gene            9224..9454
                     /locus_tag="VGM72_06685"
     CDS             9224..9454
                     /locus_tag="VGM72_06685"
                     /inference="COORDINATES: protein motif:HMM:NF022768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3126 family protein"
                     /protein_id="HEY4264986.1"
                     /translation="MTRSEIWRIEKYLRNLFRLDTITLLERPQSDSVEVHVGGEFIGL
                     IFRDEEDGEISYAFNMSILEMDLPEPPASRAG"
     gene            complement(9544..10272)
                     /locus_tag="VGM72_06690"
     CDS             complement(9544..10272)
                     /locus_tag="VGM72_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264987.1"
                     /translation="MSRSRDEFGGPAQQHSGWLIPLVVFFVTAFLSALLLVYYFGPAP
                     PGLAQEQPAPTDATRMIALSVGTMGLRIPANYIAMASARRGGVLNELALIATLPDLQG
                     YTLDAAPDLTANSPDSRVVNIMLESGKRVLPEQERLDRIYFVQMDDAKGKPGPYDLRQ
                     YAFRADSGYHDQDLFVGATDAGPAVFLCTKLAADVVSPSCMRDLPFGDGLSLSYRFKR
                     GHLGEWRRIDKNMRVRIAAFVDKS"
     gene            10453..10749
                     /locus_tag="VGM72_06695"
     CDS             10453..10749
                     /locus_tag="VGM72_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264988.1"
                     /translation="MLHALWVMLFSVASGFTASAIVANVYRVSGMRAETSGGKALRSA
                     VLVFAGPSVLFETAMKGILAKTWRPFTFWLATALILYWSLALGLIVLVIALSCQ"
     gene            complement(10766..11512)
                     /locus_tag="VGM72_06700"
     CDS             complement(10766..11512)
                     /locus_tag="VGM72_06700"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY4264989.1"
                     /translation="MARRTARNKMRDRGHEESANIHTLYPPSTGWTPLENDVRDRKYV
                     KNVRAMSALQQTLIDAIEERSVIVALGPAGTGKTYLAIAKAVDALDAGRVTRIVLSRP
                     AVEAGESLGFLPGDVNEKLAPYLRPLYDALTERLGMKRLKTLLAEGIIEIAPVAYMRG
                     RTLNDCFIVIDEAQNCTYGQIKMLLTRLGWRSTMVLTGDPDQTDLLPGLSGLRDIAGR
                     LERVESIAVVRLGDADIVRHPLVGEMLGVL"
     gene            11708..12253
                     /locus_tag="VGM72_06705"
     CDS             11708..12253
                     /locus_tag="VGM72_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEY4264990.1"
                     /translation="MPIYTIDDDRPELPPEGDYWLAPNATLIGRVRIAKGVSVWFGSV
                     LRGDNDWISIGEKTNIQDLCVLHTDPGILLSLGANVSVGHGAVVHGASVGDNTIIGMG
                     ATLLNRCRIGRNSIVGANALIPEGKEFPDNSLIVGVPGKAIRSIPESEVPMLVMNAEI
                     YFERWQLYRASLKRVDGNAIP"
     gene            complement(12250..12831)
                     /locus_tag="VGM72_06710"
     CDS             complement(12250..12831)
                     /locus_tag="VGM72_06710"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HEY4264991.1"
                     /translation="MSRAWTINAGGQLYGPFSSERMRGFASEGRLAPNSQVARENATD
                     WHEARDEPEFADLFALPPPLANPSTSPANPRPVLPSSPAPVLSRNPAPTNAAGPEGQR
                     TQFAVIVDVKSLGSGNLEKTIASFGPSYRLLPNVWLVSTEQSVNALRNRLIQELGKSD
                     SLFVVDATRGKAAWFNFGPDADVNIRRVWQKPA"
     gene            complement(12999..13682)
                     /locus_tag="VGM72_06715"
     CDS             complement(12999..13682)
                     /locus_tag="VGM72_06715"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEY4264992.1"
                     /translation="MWKKKTLALVTAVAMAGSAVQPVLAQGYDRGYDNNSNDTRGNDP
                     YNSQYDNSQYNNAPNDQDRYAQDRRNYEADRRADARWDRDAYYRNCERQRGGNTAGGA
                     IIGALAGGLLGNSVSRGPQRGAGTAVGAILGGVVGASIGNSLNCEDRSYAVDTEYRGF
                     EAGRPHQRYDWRSPRGDSYGYVQVGDYYQERGRRCATYTQEIWVHGRPETARGRACRQ
                     PDGTWQMIN"
     gene            complement(13945..14112)
                     /locus_tag="VGM72_06720"
     CDS             complement(13945..14112)
                     /locus_tag="VGM72_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4264993.1"
                     /translation="MVSRLFKQAQRLRSRARWYRMFAKLGDSTRRPDREALAEKFDQM
                     AQKAEEQASER"
     gene            complement(14220..16010)
                     /gene="sdhA"
                     /locus_tag="VGM72_06725"
     CDS             complement(14220..16010)
                     /gene="sdhA"
                     /locus_tag="VGM72_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEY4264994.1"
                     /translation="MSAAYPVTDHTFDVVVVGAGGAGLRATLEAAKLGLKVACITKVF
                     PTRSHTVAAQGGISAALGNMSEDDWRWHMYDTVKGSDWLGDQDAIEYLCRHAPEAVYE
                     LEHFGVPFSRMEDGRIFQRAFGGMTLNYGKEQALRTCAAADRTGHAMLHTLYGQSLRY
                     DVNFFIEYFALDLLMEEDVCRGVMAWKLDDGTLHRFRAHRTILATGGYGRAYFSCTSA
                     HTCTGDGNAMVLRAGLPLQDMEFVQFHPTGVYGAGVLITEGSRGEGGYLTNSAGERFM
                     ERYAPNAKDLASRDVVSRSMTIEIREGRGVGPLKDHIYLHLSHLDPKVIHERLPGITE
                     SARIFAGVDVTKEPIPVLPTVHYNMGGIPTNYLGEVLTLKNGDPDRIVPGLFAIGEAA
                     CVSVHGANRLGSNSLCDLVVFGKAAGARAAEGLMTGENHPDLRKDAGDAALARLDRFR
                     LAKGKTPTAELRLAMQRAMQEDAAVFRTGETLQSGQRRVRDIIRALPEIGVSDRGMIW
                     NTDLVETLEFDNLIAQAVVTIDSAATRTESRGAHAREDYPERDDTNWMKHTLIWFDGE
                     TAQTRIDYRPVHNYTLSNDIQYIPPKARTY"
     gene            complement(16010..16393)
                     /gene="sdhD"
                     /locus_tag="VGM72_06730"
     CDS             complement(16010..16393)
                     /gene="sdhD"
                     /locus_tag="VGM72_06730"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEY4264995.1"
                     /translation="MTARLRTPLSRIEGLGAGRSGTMHFWHQRMTAVALVPLSIWFVA
                     SALAYVGADQGAVAAYFAEPVNAVPMFLFVVASTYHMSLGLQIIIEDYIHQEGQKIAL
                     LMLNRFAAWAIGAASGFALIKMALH"
     gene            complement(16390..16824)
                     /gene="sdhC"
                     /locus_tag="VGM72_06735"
     CDS             complement(16390..16824)
                     /gene="sdhC"
                     /locus_tag="VGM72_06735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEY4264996.1"
                     /translation="MADAPNSPGPGASPFGRPLSPHLQIYRWTITMAASITHRVTGIA
                     LAAGTLFLAWWLIAAASGPDAYGLFMRLATNLLGEVVLFGFLWALAFHLLNGIRHLAW
                     DVGYGFKVPTAKLTAALVYGVSLLLAAGAFVVGIMVRGGLGT"
     gene            complement(16924..17742)
                     /locus_tag="VGM72_06740"
     CDS             complement(16924..17742)
                     /locus_tag="VGM72_06740"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY4264997.1"
                     /translation="MRRIAQLSDLHFGSHETHIAADLIASLNLQQLDLVVLSGDFTQR
                     ARQAEFAQAHRFLGNIAQPKLVVPGNHDLPLYDMIGRLHKPLKNFRRYIAPQGIAGDL
                     FQDDVIAVLGLNTARRFPGKSGRVSFDQIAHLRRVFANVPRGVFKALVTHHPLGYPAG
                     ETPHGIAGRSGHVLQALAGLDVHLLLSGHGHRPLSGTLELETAERRSILLLHAGTAIS
                     TRTRDGHGNTYNLVDIEGDGVSIRILEWSDGHGFRERQKNDYRIADGVWSRIPG"
     gene            complement(17754..18665)
                     /locus_tag="VGM72_06745"
     CDS             complement(17754..18665)
                     /locus_tag="VGM72_06745"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY4264998.1"
                     /translation="MAVLVSESAGTVTRLGAAKLEQELRAAFDAAGITAAISLVPGAQ
                     LRSAAEQALRQVQAGQFDAVAAGGGDGSIRTVAGVLADTGVALGILPLGTLNHFARDL
                     AIPLDIGAAVAVMSTRAVRNVDIGTMNGEVFLNNSSIGLYPSLVLERERQRRRQHLAK
                     RSAMLLAILLMLRHLPLFRLSVRVEGLAESCRSPLVFIGNNDYQLTLPGLGKRARLDG
                     GELSVYVTKAQSRLALFRLACRAIFGLSQRLSDMRIVKGRTASIETRRHHVLVAFDGE
                     VELLRPPLHYEIRPRALRVFAPVRNDA"
     gene            complement(18689..19378)
                     /locus_tag="VGM72_06750"
     CDS             complement(18689..19378)
                     /locus_tag="VGM72_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY4264999.1"
                     /translation="MTPARTRELNFLALAAIAALLFVFGVLAENVIQAQPFAFDNAIL
                     LALRVPGNRAIPIGPAWLPETARDITSLGSIPVIGLLLLAVTGYLLLERKRGSARWIL
                     AAAIGGVLLDDLLKFAISRPRPDMIDASVRVFSTSFPSGHASLSAIAYLTLGALLAQT
                     HDSIPIRVYILALAAALTILVGLSRLYLGVHYPSDVLAGWCIGGAWALACWSAMRWLQ
                     HPSPVDSPARP"
     gene            19675..20331
                     /gene="rpe"
                     /locus_tag="VGM72_06755"
     CDS             19675..20331
                     /gene="rpe"
                     /locus_tag="VGM72_06755"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY4265000.1"
                     /translation="MARPVRISPSILSADFARLGEEVRAVDVAGADFIHIDVMDGHFV
                     PNLTIGPVVVKALKAHSQKLFDCHLMIAPADPYVAAFAEAGADIISIHPEAGPHPHRT
                     LQFIRSLGKKAGYVLNPGTPIEVLDPLFDMIDLILIMTVNPGFGGQQFLPGQLQKIEA
                     ARKRIAASGREVMLEVDGGINHETAKLAIMAGADVLVAGTAIFHGPDDYASNIARLRG
                     "
     gene            20294..22093
                     /locus_tag="VGM72_06760"
     CDS             20294..22093
                     /locus_tag="VGM72_06760"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEY4265001.1"
                     /translation="MTMPATSLASAAKREAAEDGAPAAPRPMRERMPSGWALWIEAAL
                     VALRAAPMPLVLWFREGWLYRQTLRGPVPDRILFYPSDTRMRRLDDADGFMRGRFRLG
                     GQKLEIRQGSVFDAPPPGDSFAAALHGFDWLRHLEAAGGDLARGFALRLTQQWVKRYA
                     RFSPFAWRPEIIAERFLNLFAHGRFFLANSDLVWRSKLFVSLRNQAQMLSRTAGEAPD
                     GLPRLKAAAALALAGVCLGDVHHEAAGLKHLDGEIARQILPDGGHISRSPQILLEAYR
                     VLAMVQQALDHTSARNDAVLRGALDRMAPMLRFFRMGDGALAVFHGGGESLSRTLEAT
                     LDSDDALGRPLGHAPYSGFQRLAAGRTLVVLDVGAAPEGAFSTAAHAGCLALEMSVGT
                     NRLIVNCGMAIDDSEAWKTALRSTAAHSTLMIDDTSSATILGEGTLTRFLGPRLVEGP
                     AGVETERSESAQGLTVAAGHDGYRVPFGIIHHRHMTLSPRGASLTGLDRLVPAGPEHA
                     ARNAARRPFTLRFHVHPDVRLSLAQGGGSVILKLPNGEGWRFRCSGGALSVEESIYFG
                     GGTARRAEQLVVSGAWTGEALECGWLFEQVRSS"
     gene            22104..22799
                     /locus_tag="VGM72_06765"
     CDS             22104..22799
                     /locus_tag="VGM72_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4265002.1"
                     /translation="MMEFLRRILGLSGSKGASFPRQCNVCGYRGRFRAAGKPRRIDAR
                     CPRCGSAERYRLLALWLDRHGAGLAQAKVLHFAPEAGVTAMLKTRVGHYESADIAPGR
                     ADRVLNIEAIAAPGASYDCVVCSHVLEHVDDAKALLEIMRVLKPGGVVLIMLPVIEGW
                     AKTYENPAVQSPEDRKRHYGQADHVRYYGADVRTRIRGAGFALKEFTAEGPDVLTYAL
                     QRGEKVFIATKPA"
     gene            complement(22822..23547)
                     /locus_tag="VGM72_06770"
     CDS             complement(22822..23547)
                     /locus_tag="VGM72_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM sorting domain-containing protein"
                     /protein_id="HEY4265003.1"
                     /translation="MAPITSGLLTGNEVIIGGFAAGTLLVDQSASVNAAGANVHVSGG
                     FNGTQTTAPGTLTVKNGATLTADTVTIYQNGTLNGNGTVDANVVLSGGAISPGNSPGT
                     ETINGNLDLASGTLNLEIGSDILADHLNVSGNVIIGTDVVFNLIFEYVPTFGKAIDLD
                     DFFSLTGGGTLAFAPSFDLASQFNVQGLTAGSEIILTADGQRSVFAAAAAAVPEPASI
                     ALLLAGIGGLAAVRRRRKTAGLI"
     gene            complement(23520..23885)
                     /locus_tag="VGM72_06775"
     CDS             complement(23520..23885)
                     /locus_tag="VGM72_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265004.1"
                     /translation="MSFDDKILSAVEALYDAALDETLWPQAMGQIVEIPGSHAATFCV
                     IDGSERPRLPVFATFNFEQRFIDDYLESMVFHDPTVQYIVAHPDRKIIHDAEFISERE
                     KERRVPARTSRWHRLPQVS"
     gene            24040..24834
                     /locus_tag="VGM72_06780"
     CDS             24040..24834
                     /locus_tag="VGM72_06780"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011279414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEY4265005.1"
                     /translation="MVQLNLPKESRVKPGKTWPLAKRVDGKPTKRAKRIRIYRYDPEI
                     KANPRLDTYSVDTEDCGPMVLDALIKIKNEVDPTLTFRRSCREGVCGSCAMNIAGANT
                     LACTKAMADVSGSINIYPLPHMPVVKDLVPDMTGFYAQYASIGPYLQTKSGTPPKEWL
                     QSPEERAKLDGLYECILCACCSTSCPSYWWNPERFLGPAALLQAYRWLADSRDENTGD
                     RLDHLEDPFRLYRCHTIMNCANVCPKGLNPSEAIANIKQMMVKRAV"
     gene            complement(24857..26065)
                     /locus_tag="VGM72_06785"
     CDS             complement(24857..26065)
                     /locus_tag="VGM72_06785"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4265006.1"
                     /translation="MSPSRITDAALSEAHHHFEAGRLEEATKLYRGHLRSHPKSSEAF
                     EGLGRVCLQAGQFERAQYFFSEAARFDPVSLDALRLRGAALMQLGRHAAALDCFERAL
                     AVKPDFIEALVNRAVALFEMKRLDEALTGYDRVLALDPQNAVAWTNRGNVLRTRQQLD
                     EAVACYDRALAIQPDLETAKQNRFYVLLELRKVSRIDGRALRETFDDISPRFDALMVD
                     KLHYRGHLQVRTLAERVIVPLAAPLTILDLGCGTGLVGEAFKDVAADGGRLDGIDLAP
                     RMIEAARSRGIYDDLILGDLENVLAAPGLSYDLILSADTMVYLGDLAPAFSGVARRLN
                     PGGFYIFAVESKTGESWEQTPQNRFKHSEAYLREEGARAGLSFVDSMQTTLRFEADQP
                     VPGLVAALRK"
     gene            complement(26069..27292)
                     /locus_tag="VGM72_06790"
     CDS             complement(26069..27292)
                     /locus_tag="VGM72_06790"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4265007.1"
                     /translation="MNEILMQRARGLHQAGRLKEAADLYQKILLADPAQFEAFYSLGL
                     IAVHTGQLEEAQQLISNAMMLNPGFAEGWCARGLVLVQLKRHQQALSSFDRCLSLKPQ
                     YADALSSRATTLLELNRADEALAGFDAAIALDPRHAISWNNRGNTLAGMGRFAEAIES
                     YERALALMPDLPQAKENRDNALFELKRASRCPPGYMRNLFDDFAPHYDETMLQKLGYA
                     AHLHLRTLGNCVLPRDKSAWRILDLGCGTGLVGDVFKDLSAGGRLDGIDLAPRMIDAA
                     RARGIYDELILGDLESVLPAPGPRYDLILAADTMIYIGDLAAIFRGVAARLEPGGFYL
                     FAVESKQETDWEQTPMRRFRHSESYLRRIAAEAGLSFVDIAPCALRQEAAASVPGFAV
                     ALQKPIVPAADSGTD"
     gene            complement(27307..28635)
                     /locus_tag="VGM72_06795"
     CDS             complement(27307..28635)
                     /locus_tag="VGM72_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4265008.1"
                     /translation="MSDPLLLDAQQASQEGRLADAARLYHDFLNANPVHGPARYALGM
                     IYFQQSQFDQAEYLFGEAVRCDPSFADALCMRGVALMKLSRADEALASFDQALGVKPD
                     FIEALSNQATTLLELGRLHEGLAGLDRVLVLDPGHVLSWNNRGNALFALKRYGEAIES
                     YDRALAIYPDFPDARQNRLLALGEMNRGGLRFAEILCQQGEDMMRREQFGEALTRFEE
                     ALKLRPDYPEAHGLHTIAYRQAYRRLFDDNALLFEDSLLNNLNYRGHLQLREMADRVW
                     PDARFGLRVLDLGCGTGLAGDQFHAWTTGGGRLDGIDFSPRMLDEARKRGIYNNLYLG
                     EIEQMLGTGGEIYDLMIAADTLIYFGDLNAILQGASAHLLPGGFFIFTVESKDGDGWE
                     PTPKRRFRHSESYVRECAGRAGLAFVDRVESALRFETGAAVAGFTIALRK"
     gene            complement(28689..>29553)
                     /locus_tag="VGM72_06800"
     CDS             complement(28689..>29553)
                     /locus_tag="VGM72_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265009.1"
                     /translation="MTDGVVYEAGWTLDDVDWSRFDPSKVKPALLAAVKAASLVEYNA
                     PDYVTYLKRVYSGTPESTLRSIEHWGEEEIQHGLALARWAELADPCFDFITAFTRFRS
                     LYQAAHFSGGKGSVRGSRRGEMIARCVVESGTSSLYSAIRDASDEPVLQEVAGRIAAD
                     EFRHYRLFYDILQDETEPDLPLWRKLWVAVTRVNESDDDELACAYYCANVSPAEADAK
                     PYRRRIYARAYQQRIMAFYRRHHIDRLVKMVVKPTGLNPSGRLSRMASALLWRLLSLR
                     GGADAGRFKPV"
BASE COUNT         5609 a         9087 c         9244 g         5613 t
ORIGIN      
        1 gcatagggat cgtccgcgat ggccagctcg gtcacctgca gccgcttgat ctcgtcgcgg
       61 agccgtgccg cctcctcgaa ttcgagatca ccggcagcgg cgcgcatgcg cttttccaat
      121 tccgcgatat gggatcgcag attgtgcccg atcggcacgg ggtcgtccgc aaggcccgaa
      181 tccaccagca cgtgatcgcg ctcgtaggcc gagcccatga tgtcggtgat gctcttcttg
      241 atgctgagcg gcgtgatgcc gtgggcgaga ttataggctt cctgccgggc gcgccgccgg
      301 ctggtttcgg ccatggcacg ttccatcgag ccggtgatat gatccgcata cagaatgacc
      361 ttgccgtcga cattgcgcgc cgcgcggccg atggtctgga ccagcgaggt ttcggagcgc
      421 aggaaccctt ccttgtcggc gtccagaatg gccaccagcg cgcattcggg gatgtccaag
      481 ccttcgcgca ggaggttgat gccgaccagg atgtcgaagg cgccgagccg aagatcgcgc
      541 acgatctcga tgcgctccag cgtctccaca tccgaatgca tgtagcgcac gcgcacgccg
      601 ttctcgtgca gatattcggt caaatcctcg gccatgcgct tggtcagcgt ggtgacgagg
      661 gcgcgatagc cggccttggc gatgagcttg cactcggcca gaaggtcgtc aacctgatgt
      721 tcgacagggc ggatttccac aggtgggtcg atcagaccgg ttggccggat cacctgttcg
      781 gtgaacacgc cgccggtgcg gcccagctcc caaggacccg gcgtcgccga gacggagacc
      841 gtttgggggc gcatcgcatc ccactcctcg aatttcagcg gccggttgtc gatgcaggag
      901 ggcagccgga agccatattc ggccagcgtc gccttgcgcc ggaagtcgcc tttgtacatg
      961 gcgcccaatt gcggaaccgt gacgtgggat tcgtcgacga agacaattgc ctcgtcgggc
     1021 agatattcga acagcgtcgg cggcggctcg ccggggcgcc gtccggtcag ccagcgcgaa
     1081 taattctcga tgcccgcgca ggagccggtg gcctcgatca tctccagatc gaacatcgtg
     1141 cgctgttcca gcctttgggc ttccagcagc ttgccttcgc ggcggaactg atcgaggcgc
     1201 aggcgcagtt cctcgcgcat gcccttggtc gcctgcgcca gcgtcggacg cggtgtgaca
     1261 tagtgcgaat tggcgtaaat cttcaccgtc tccatctcgg cggatttctg ccccgtgagc
     1321 gaatcgaatt cgtagatcga atccacctcg tcgccgaaca gagtcacgcg ccaggcgcgg
     1381 tcttcataat gcgccgggaa aatgtctacc gtgtcgccgc gcacacggaa cgcgcctcgc
     1441 gtgaacgcct cgtcattgcg gcggaactgc aacgcaacca gacgcgccaa cagatcgccg
     1501 cgggaaacct gctgtccccg ggtgatggaa aacgccatcc cggaataggt ttcaaccgcc
     1561 ccgatgccgt agatgcagga caccgacgcc acgatgatga catcgtcacg ttcgagcagc
     1621 gaacgagtcg ccgcatggcg catgcggtcg atctgttcgt tgacgctgga atccttctcg
     1681 atataggtgt cggtgcgcgg gatataggcc tcgggctggt agtagtcgta ataggagacg
     1741 aaatattcca ctgcgttctc ggggaagaat cctttcatct ccgaataaag ctgggcggcc
     1801 aaagtcttgt tgggcgcgag caccaaagcg ggacgctgca gccgctcgac aatcttcgcc
     1861 atggtgaagg tcttgccgga acccgtcaca cccaagagaa cctgatcgcg ctcgggtgtg
     1921 ccgctcggcc cctgaccgca aagcccctca gccagctccc ggatggcctc gggctggtcg
     1981 ccggcgggct ggtagtcgga gacgagcttg aaccgcttgc cgccctcgga tttctcgggg
     2041 cggaccgggc gatgcggcac gaaggccgtc atctccgcgc ccgtcatgtg acggaacgcg
     2101 gccggagctt cagccagacc gctcaccggc cggaaggcag gaccattatt gtgcgccatg
     2161 gaggcaatat aggggagacc atcggcttat gcagcgggtc atccacaaaa cgatgcgggc
     2221 gcatgccagc ctggcacgcg cccgatcatc ctgggtacga acgctatttc aacatcgcct
     2281 ggatatcgcc ggcggtcttc tttgcgcccg ccatgtcgtt gttcgcgatt ccggccttgg
     2341 cttcggtcgc cgcggtttgc agctttgcct tcgtcgccgc atccgccgtt tgggggatgg
     2401 cgccgccgcc cgcattcgcg cagggattgc cgggggctgc gtcgaagcca tcgccgccgg
     2461 gaccgaccaa gcaattcagc acgtgatgca ggtgggtgtg caccatgtcg atgccgcctg
     2521 ccgccgcggc aaggcccgca tgggctgcgg cggtgttgat ggcctgtggc ccatcggcca
     2581 ttgcgacggg tgcaagtgcg gtcatgctgc caatcgcaaa ggtgcaaaaa atagcgcgtg
     2641 agatcttcat gttatcctcc gttagcactc aacgaatttg ctattctggc acaaatcatc
     2701 gccggcgcga aagatgtgcg aaaatcactt gggttggccc tcatggtcgc cggtgcgcgg
     2761 aaaggaggtc ttcaattgtt cggcgatgtc gaagtcgagt tctgcatcct cccagtgtgc
     2821 gaccatcaat tggtcgatcg tgacgtgggc gtgctttaaa tcggtgctct gcaactgggc
     2881 ctctgccatc gtcgcaccct gcaattgggc gctctcgagg gaggcattct gtaatcgggc
     2941 ctggtaaaaa tcggcattgc gcaattgcgc gaatgccagc actgcacttt gcagattggc
     3001 cgcagagaat tccgcgtcat ccgccagcac ataggggaac ctggcgccac gcagattggc
     3061 ccccgaaaag ctggcgcctg tgatatcggt gctagccggc aacatgccca gttcatttga
     3121 atcttccccg gcagtccggg cgacgctgag gtcgcgcaac tgattattgc tgagatcagg
     3181 cctgaaatcg gcggcgatga gcgtggcatt ctcgaaattt gcgtaagata ggttgcgccc
     3241 gcgcaagctg agggccgcgt tgcggatgcg ctcgacggtt cgctgcattt cgggcgacac
     3301 cccttcgata tcgtcgcgca gcgcgatgac aattgtgtcc tggttcagcg cttgcagcaa
     3361 ttcctccgaa ggtagctccg cccaaagcgt gcggtccgca agattgaggc tgcggcggat
     3421 gccgaatttt tcggcgaaat cggtgccggc gagcggccag aatgcctggg tttgcgaatg
     3481 cgtcatgctc caaagcgtgc gccagaacac atcctggggc tcaccgggaa aggccagcac
     3541 aatcgtggag accaatagcg gaagaacgaa caatgcgctc atcgccatcc ggtgcagtct
     3601 cagtcgcgac gtccaaccgg ccatggcgcc ggccgcctgg gtggcggcag gcgcccgctt
     3661 tgcgcgtagc cagagctgga tataagcaat caagcccaga tcggcgatga cgaatccgcg
     3721 ctgtagccaa gtgatcttcc catcgtgata gggcaggaat tgcaactgta cgaacaggag
     3781 caagagcacc ggcataaaga aaatcgtgct gtcgcttacc aacgccagaa gccagcgctc
     3841 gcgccggtcc gtgaccaggc ctgctttcag catggcgtag tcgaacgcca tcagcctgcg
     3901 gcgcaggcgg ttgcgttcga attgcgcatt ggcgtcgtcg caatagttga gcggcaggat
     3961 cagctcgctc acgcgcacgc gcaaatcgtg aagcttcatc aggagattga gatggaacag
     4021 caccagcagt aacggcgcga tgacatagaa cagcagcagc ggtatgccgc tgtccacatt
     4081 gaacagcggc aatttcacgg gcgtgcgtcg cagcagcgct tcatgtgttg taccgctggt
     4141 gagaacgaag atggtcgccg catagccgat aaaggtcgcc cacaacgtgc ggcaatagcc
     4201 tgcggcatcg ttgatggcgc cgatggcggc ctcatccacg ctgggatcgg cgatgctcga
     4261 cataaggtcc cttcagcgag tcaccctcag ggtacaaccg atcgggaaaa tcatgaagga
     4321 ggtgactacg gcttcacccc gatgacggag gaatcgtcca cccggaccca gaagatcgga
     4381 atacgttgcg cggccccgta ttcgtcgacc gccttgcgtg caccctgcca gcggaaataa
     4441 tcgtcgatga tgacgatgcc tccgcgcacc agcttcggat agagatgatc gagttcggct
     4501 ttggtcgagg cgtaccaatc ggtgtccagc cgcaacaatg cgatcgcccc tggcggtgct
     4561 gccgcgggca gcgtgtcttc caccttgccc ttgatgaaat gcagccggtc gggcggatag
     4621 ccggtcaagg taagattagc gcggacgtcg tccagcccgt ggttgatcca ggtcgagcgg
     4681 cccagttcgt cgcgcctttt ccgccagctc cgggatgcgg cctcgccgaa ccgcccgcgg
     4741 tcgacatcct cgggttccgg catgccttcg aacgtgtcgt agagatagag ttcgcgatcg
     4801 cggataccga gttccagcag cgtataggcc atggccatgg tcgaaccgcc tttcgccacg
     4861 ccgcattcga cgaaggcgcc gggataattt tgcgccacga catgacgtac cgccgattcc
     4921 agcaccgcca cgcgctcgtt cgaggtcatc gtgtaaggcc gcacctgttt gagcaacgtc
     4981 ttctgggcgg gggaaaaatc gagcgggatg gacggcggaa gattgcggcg aaacgggcgc
     5041 gtcagctcgc gcttcaattt agccacccaa tcgaccatgg atcccccgcg ccccgcttca
     5101 ctccggacct tcgatagggc gtgcccggac ccggatcaat gggccggttg tgcggggcgg
     5161 cggaacgccc atataagcga aggaacaaaa ggccccgcgc atgagcgaga ttcctcccgc
     5221 aaacccgccg aatccggccg cgccgcatta cgatgcggcc gggggcgcgt ccgatctgcg
     5281 ctcgctggca atcgtctgct acgtgttgtt cctgcttgcc agcgtgaacg ggctttcggc
     5341 catcgtcggt gtcatcattg cctatgtgaa gcggcgtgac gccgctggca ccctctggca
     5401 gagccatttc gccaatatga ttctggtgtt ctgggtgacg gttggggcgg cgattctcgg
     5461 cttcctgaca tggcctatcg cgctcggcgc attcttcgtg gcgtggccgg tcttctggcc
     5521 gccggtgctg actttgccat tcgccttcgg gtttttgttc tttccgctgc tggtgctctg
     5581 gtatctctac cggatcgttc gcggcctcat acgcgctagc gaagagcgga cgtattgaga
     5641 agaatggcct attgaaagaa aagcccgcgc tcggattgag cgcgggcttt cttcgtcaac
     5701 acaacgcgtc agacggttta aggaaatttg aagctgaagg tcgccacgta gaacggcccg
     5761 gtggcaaaat atggagccgt gtagctcgaa aacgtacgtg tgtcggtgtc gagatagcgc
     5821 agatcgaggg aatatttgtc ataggtgagc gtcacgccaa catcccattc gacataggaa
     5881 tcgtcggaaa ccccggcggg ttttccacct gaatagccct cataaccgac attgccgctg
     5941 agcgagagcc agggcgcgat cgggaccgaa acgccggtcg tgaggaaata ttccgaatcg
     6001 gggttgtagc cgttgcggac ggtgagataa ccgctgggaa gcgagggcgc gtaaaacgcc
     6061 ttggccgacc aggcgagaac gccgaaatca tggctcaatc cggcatcgag ctcgaacaca
     6121 tcgtagggtc cgccatttcc cgaatagggg tagacatagc catagacgcc gaccgaggcg
     6181 ccgacgggac cgagattgaa attgtaattg ccgtagatgt cccattccaa attcgaatcg
     6241 tccccgaaat tgatggacga gacccaggta ccgacggcaa agccggtgtc cggaacggca
     6301 taatcgagcc cgccctggac tgccggacgt ttggcgcttt gggtgatgcc gcggaagata
     6361 taatccgtgg tgatcgcggc gttggcggtc agcccatcga tcgcgaacgc gggcgacgtc
     6421 catgccgaag ccgaagccaa gccgagggcg ccaagcgcaa tctgcatagt ttttttcata
     6481 atcactcctt ctttgctatc ccggcggcac tggcctccag gcgggtcccg gacttcacac
     6541 agcaggccga tggacatcgg agttagagat gaaggtctcc cagaatcaat tacgctgcac
     6601 tgcaaaaatg ttgtatttca gacacaaatc agaccatttt ttcatcatat ggctctccat
     6661 gacaaaaata tgcctattat ttaggcgaat agcctctgca ataggcctcc gccgcattgc
     6721 atgcgtcact tctggaagca tgcatcgtgc ttgggacgaa gccttcacag cccggcggcc
     6781 gagagctgct ggaaatcctt ttcccggggc gatggccgcc tatatggttc gcgcatgacg
     6841 agggcagggc gaataaagat gcgttccgcg gccatacggc gatcggtatc ggccggccaa
     6901 tgacgctgac gctggagaat catccgcgcg cggcgcggat ctttgcaggc aagcccgcac
     6961 ggctcatcct ggtgcggcat ggcaagcctg ccattcccac caatccgcgc accagccacc
     7021 acggatttcg tcgctatatc gatgcttatc aggatgcggg gctggacccg caaagcgcgc
     7081 cgccggagga actgctcgac ctggtgaagg ggctgaatgc cgtattcacc agcaatctgc
     7141 cccgcgccaa tgacagcgcg cgcaccctgc ttccggaagc cgagattatc gccgacccgc
     7201 tcttcaccga agcgccgctg gccgttccgc gcattccgct cttgaaaatg aaagtgccga
     7261 tttgggccgt catggcgcgc atcatgtggc acgcgggcta tcaccccgaa atcgagaatt
     7321 atcgtcgcgc caagacgcgc gcctcacagg ccgcggatat cctgctcggc cgcgccgaag
     7381 ccaatgatgg cgtcgccgtg ctcgtcgccc atggctattt caatgccatg atcggacgtg
     7441 tcctgaagaa gcgcggattt tcccgtaccg gctcgcaccg catccgcttc tggaacgcgg
     7501 tgatttacga gcgcgcgtca tcgtcttgac gtcacaccgc gtcagaattt cgtggtcatc
     7561 gaatcaaccg gcggccgcgg ccggtcctcc agcgtgtcca ccggatggcc gatatagatg
     7621 taccctgcaa tccgctcgcc aggcttcagg ccgatgcgtt ccagcacctt gggatgatag
     7681 gcacaccatt cggtgatcca attggcgagg aagcccatgg cgtgtgcggc gatgacgatg
     7741 ttcatgcaca cagcacctgc cgaaagctcc tgctcccaga tcggaattgg gatttgctcg
     7801 cgcgtgcggc tgaccacggc gacgatgacc ggcgcacgca ggaaacggcg gcgttcctgc
     7861 tcgacgcgtt cgggcgagga atcgcgttcg ccggcgatgg tttcggcaag gatatcgccc
     7921 atccgtacgc gcccgtcgcc ttcgaacagg atgaaacgcc agggggtgag cttgccgtga
     7981 tccggcaccc gcacacccgc cgtgaggatg cggcacagct gggcggaatc gggccccggt
     8041 cctttcatgg ccttggcgga gcccgaacgc cggctgagaa gcaggtcgag cgtttggggg
     8101 acggggatgt tcagtgcaga cggagcttcg gacatgtaat atgaacccat tgaggagcct
     8161 tggccggacc ataggcgagc gtattacgcc aagaaagctg ctttggcggg cagtgcgcaa
     8221 aaaaccgcgt ttcgatcttt gcaggccccg ggctaagatg catgaaacct gtcgtggatc
     8281 gcatattgtc taggaagaac cgatgacccg ggaccggaac atgcgcgctg ccgaaaaagt
     8341 cgcgagctgc gatccgatat ggtcggcgct gcgtgaacag gcggaacagt tggcgctgtc
     8401 ggagccggaa ctggcgagct ttgcccatgc cacgatcctg aagcacgacc gcttggaggc
     8461 ggcgctttcc tatcacctgg ccaagaagat cggcggcgaa gatctttcgc cgatgcaggt
     8521 gcgcgagacc ttcgaggaag cattcgccgc cgatccggcc atcggtcagg ccgttcgcgc
     8581 cgatctttcc gcggtgttcc agcgcgatcc ggcgtgccat tcctacatcc aggcctttct
     8641 gttcttcaaa gggttccatt cgctggaaag ccaccgcatc gcccattggc tgtggcgcag
     8701 cgggcggtcg tggatggcgt atttcttcca gaaccgggtc tcggatcttt tcgcggtgga
     8761 catccatccg gcggcgccgt tcgggcgcgg catcttcatc gatcatgcga cgggcctggt
     8821 ggtcggagaa acagccatgg tcgaagacga tgtctcgatg ctccatgggg tgctgctcgg
     8881 cggcaccggc aaggaaagcg aggatcgtca tcccaaggta cggcgcggcg tgctcctcgg
     8941 cgcgggcgca aaggtgctcg gcaatatcga ggtcggcgaa tacagccgcg tcggctcggg
     9001 cagcgtcgtt ttgaaatccg ttccgccccg caccacggtc gccggcgtgc ctgcgcgggt
     9061 gatcgggaag gccggatcgg atcggccttc ccaagatatg gaccattgcc tgccggagcc
     9121 tgatctagga acctagtcct tgcatgctgc gagggggtga acacgtacgc ttgacccatg
     9181 cgcgggagcg tgcgacgtca atctctctta agggagcaga gctttgaccc gcagcgaaat
     9241 ctggcgcatc gaaaaatatt tgcgcaatct gttccggctg gacaccatca cgctgttgga
     9301 gcgtccccaa tcggattccg tcgaggtcca cgttggtggc gagttcatcg ggctgatctt
     9361 cagggatgaa gaggacggcg agatttccta cgccttcaac atgtccatcc tggaaatgga
     9421 tctgcctgag cccccagcgt cgcgcgcagg ctaaagcgcg gtgtttcaat cggccacgcc
     9481 ataattcaag tctatgcatg tgacgccatc agggaatcgt cccttgatga ttccaaaact
     9541 gctctagctc ttgtctacaa aagcggcgat gcggacgcgc atgttcttat cgatgcggcg
     9601 ccattcgccg agatgcccgc gcttgaagcg gtagctgagc gaaagaccgt ccccgaacgg
     9661 caggtcgcgc atgcagctcg gcgagaccac atcggctgcg agtttggtgc aaagaaacac
     9721 cgccggtccc gcgtcggtcg cgcccacgaa caaatcctgg tcatggtaac ctgaatcggc
     9781 gcggaacgca tattgccgca agtcataggg gccaggcttt cccttggcgt cgtccatctg
     9841 cacgaaatag atgcggtcca gccgttcctg ctcaggcagc acccgtttgc cgctttcgag
     9901 catgatgttg acgacgcgcg aatccggact gttggcggtc agatcgggcg cggcatcgag
     9961 cgtgtagcct tgcagatcgg gcagcgtggc gatcaaagcg agttcgttca gcacgccgcc
    10021 gcgccgcgcc gatgccatcg cgatatagtt cgccggaatg cgcaaaccca tggtgccgac
    10081 ggacagggcg atcatgcgcg tggcatcggt gggcgcgggc tgttcctgcg caagacccgg
    10141 cggcgccggt ccgaaataat agaccagcag cagcgccgac agaaacgccg taacaaaaaa
    10201 tacgacgagc ggaatcaacc aacccgaatg ctgttgggcg ggtccgccga attcgtcccg
    10261 ggagcggctc atgcttcgtg tttcctgcgt tcacgcctca tggcgtcatg ctttctagca
    10321 cggccttctg gccccaaaag agacgcttcc cgcgcgcggt cggcattttc cgctccaatt
    10381 cggcacaaaa tcgcgtgtct cggcaggcac gcgccctgca acacccggct cactctgaaa
    10441 gaggagcgga ttgtgctgca tgcgctttgg gtaatgcttt tctcggtcgc cagcggtttt
    10501 accgcatcgg ccattgtcgc caatgtttat cgtgtctcgg gaatgcgcgc tgaaacctct
    10561 ggcggcaagg cgttgcgttc ggctgttctc gttttcgccg gtccaagcgt gctttttgaa
    10621 accgccatga aaggcatcct ggcgaagaca tggcgcccgt tcacattctg gctcgccacg
    10681 gccctgatcc tctattggag tctggcgttg ggacttatcg ttctggtgat cgccctgagc
    10741 tgccaatgag gatgtgcgct cgccgttaca gtaccccaag catctcgccg accagcggat
    10801 ggcgcacgat gtcggcatcg ccgaggcgaa ccacggcaat cgattctacc cgctcaagcc
    10861 gtccggcgat atcgcgcaga ccagagaggc cgggcaacaa gtcggtttga tcgggatcgc
    10921 ccgtcagcac catggtcgaa cgccagccga gccgggtcag cagcatcttg atctggccat
    10981 aggtgcaatt ttgcgcttcg tcgataacga tgaagcaatc gttgagcgtg cggccgcgca
    11041 tataggcgac cggcgcgatt tcgatgatgc cttcggcgag cagcgttttg agacgcttca
    11101 tccccagccg ttcggtcagc gcgtcgtaaa gcggccgtag atagggcgcg agtttttcgt
    11161 tcacgtcgcc gggtagaaaa ccgaggcttt cacccgcctc gaccgctggc cgcgacagca
    11221 cgatgcgtgt cacgcgtccg gcgtccagcg catcgaccgc cttggcgatg gcaagatagg
    11281 tcttgccggt tcccgccgga cccagcgcca cgatcacgga gcgctcctca atggcgtcga
    11341 tcagcgtctg ctgaagcgcg ctcatcgccc gcacattctt cacatatttg cggtcccgca
    11401 cgtcgttttc gagcggcgtc cagcctgtgg aaggcggata gagcgtgtgg atgttcgcgc
    11461 tttcctcatg gccacgatcc cgcatcttgt tacgcgcggt tcgtcttgcc atggacacct
    11521 ccgtctgaaa gcgactcaac tgcccgaatc gaagcgtagc ctgtttgatg ggctccgtcc
    11581 cggcgttcgc aaaatattct caatcacgaa ttccgtgctc ttgtttcagc tcttgtttca
    11641 tctcttgttt ctggtgcccg ccgcgatgtc ttatgatgcg gacactgacg cgaaccgaaa
    11701 gtcccgcatg cccatttaca cgatcgacga tgaccgccct gaactgccgc ccgaagggga
    11761 ctattggctg gcgccgaacg caacgctgat cggccgcgtc cgcatcgcca agggcgtcag
    11821 cgtgtggttc ggcagcgtgc tgcgcggcga caatgactgg atcagcatcg gcgaaaaaac
    11881 caacattcag gatctctgcg tgctgcacac cgatccggga atactcctca gcttaggcgc
    11941 gaatgtctcg gtcggacacg gcgcggtggt gcatggtgcg agcgttgggg acaatacgat
    12001 catcggcatg ggcgcgacac ttctgaaccg ctgccggatc ggccgaaact ccattgtcgg
    12061 cgccaatgcg cttattccgg agggaaagga attccccgac aattcgctca tcgtcggtgt
    12121 gcccggcaag gcgatccgca gcattcccga atccgaggtt ccgatgctgg tgatgaacgc
    12181 ggaaatctat ttcgagcgct ggcagcttta tcgcgccagt ctgaagcgcg tcgatggaaa
    12241 cgcgatccct taagccggct tctgccagac gcgccggata ttgacgtcgg catcggggcc
    12301 gaaattgaac catgcagcct tgccgcgcgt ggcatcgacc acgaagagag aatccgattt
    12361 tcccagttcc tggatcagcc ggttgcgcaa agcattcacc gattgctcgg tcgaaacgag
    12421 ccagacattg ggcagcagac ggtacgatgg cccgaaggag gcgatggtct tttccagatt
    12481 gccgctgccg agcgatttca cgtccacgat caccgcgaac tgggtgcgct gcccttcggg
    12541 cccggcggca ttcgtgggcg caggattgcg cgacaggacc ggtgccggcg aagacggcaa
    12601 aaccggccgg gggtttgccg gcgacgtgga cgggttcgcc aacggtggag gcagtgcgaa
    12661 gagatcggcg aactccggtt cgtcgcgcgc ttcatgccag tccgtcgcgt tctcccgtgc
    12721 cacctgggaa tttggcgcga gccggccttc ggatgcgaaa ccgcgcatcc gttccgaaga
    12781 gaacgggcca tacagctgtc cgccagcatt gatggtccac gcacggctca tcgggcgaag
    12841 cgctccttat catttggcca acgcggtaaa ccgattggtg accgctctct tgtctcgacc
    12901 gttcacagag aagcgtcgtt tacacatgca aacgctccca gtccaatccc gggagcgttt
    12961 gccgcacgat attaaaaatc acgggatatt aaaaaaaact aattgatcat ctgccacgtg
    13021 ccgtcgggct ggcggcaggc gcgtccgcgt gccgtttccg gccgcccatg cacccagatt
    13081 tcttgcgtat acgttgcgca gcggcgccca cgctcctgat aatagtcgcc gacttgaacg
    13141 tagccgtagg agtcgccgcg cgggctgcgc caatcatagc gctggtgcgg ccgccccgcc
    13201 tcgaagccac gatattccgt gtcgaccgca tagctgcggt cctcgcagtt caagctgttg
    13261 ccgatgctcg cgccgacgac gccgcccagg atcgcgccca ccgccgttcc ggcgccgcgc
    13321 tgcgggccgc gcgatacgga gttgccgagc aagccgccag ccagggcgcc gatgatggct
    13381 ccgcccgcgg tgttgccgcc gcgttggcgt tcgcaattgc gataataggc atcgcgatcc
    13441 cagcgcgcgt ctgcgcggcg atcggcctca taattccggc ggtcctgtgc gtaacggtcc
    13501 tgatcgtttg gggcattgtt atattgggag ttgtcgtatt gggaattgta cggatcgttt
    13561 ccgcgagtgt cgttagagtt gttgtcgtat ccgcgatcat aaccctgcgc cagcaccggt
    13621 tggacagcgc ttcctgccat ggcgacggcg gtcacaagcg caagtgtctt tttcttccac
    13681 atcgtcttct ccgttcggtt cgggtgaagg ctcttcgccc tctcggtcct tccgccatca
    13741 atttggtcca gacaggctga accggaactg aacagagaac ggtccgcggc gatggaggtt
    13801 cccgcaggtt acaatgatcg ccaccaggaa gaagatgccg gtttataagg ctttttcccc
    13861 ttagcggccc gcgccgcccg cgaagggtgc tcgcgatcga ggcgtaaaag aatcgtggca
    13921 aattcgtggc cgcagcggtt tccttcagcg ttcgctcgcc tgctcctccg ccttttgcgc
    13981 catctgatcg aatttctccg cgagcgcttc gcgatccggc cgccgtgtgg aatcccccag
    14041 cttcgcgaac atccggtacc agcgcgcgcg cgaacgcaag cgttgtgctt gtttgaaaag
    14101 tctcgaaacc atgcgttccc tgacgccgaa ccacatggca tttgaacgca acgtcgcgga
    14161 gaaacgcgtg ggaattgcaa cggtggttaa atcgtaaccg cgatcccagc tgcgttaatc
    14221 taatatgtcc gcgctttcgg cgggatatat tggatatcgt tcgacagtgt atagttgtgc
    14281 accggacggt agtcgatccg ggtttgggcc gtctcgccgt cgaaccagat cagtgtgtgc
    14341 ttcatccaat tcgtatcgtc gcgctcgggg taatcctccc gcgcatgggc gccacgcgat
    14401 tcggttcggg tggccgcact gtcgatcgtc acgaccgcct gagcgatcag attgtcgaat
    14461 tccagcgtct cgaccagatc ggtgttccag atcatgccgc gatccgacac gccgatttcg
    14521 ggaagagcgc ggataatgtc gcgcacgcgc ctctgtccac tctgcaaagt ctcgccggtg
    14581 cggaagaccg cggcgtcttc ctgcatggcg cgctgcatcg cgaggcgcaa ctcggcggtc
    14641 ggcgtcttgc cttttgcaag acggaagcgg tctagccgcg cgagggctgc gtcgcccgca
    14701 tctttgcgca ggtcaggatg gttctcgccc gtcatcagcc cttcggccgc gcgcgcgcct
    14761 gccgccttgc cgaacacgac gaggtcgcag agcgagttgg agcccagacg attggcgcca
    14821 tgcaccgaca cgcaggccgc ttcaccgatg gcgaagaggc cgggcacgat ccggtcggga
    14881 tcgccattct tgagcgtcag cacttcgccc agataattgg tggggatgcc gcccatattg
    14941 taatgcaccg tcggcagcac ggggattggc tccttggtca catccacgcc cgcgaagatg
    15001 cgtgccgatt cggtgatgcc cggcaggcgc tcgtgaatca ctttagggtc aagatgcgac
    15061 agatggagat agatgtggtc cttcagggga cccacgccgc gcccctcgcg gatttcgatc
    15121 gtcatcgagc gcgagacgac atcgcgggag gcgagatcct tggcgttggg ggcgtagcgc
    15181 tccatgaagc gttcgccggc cgaattggtc agatagccgc cttcgccgcg tgagccttcg
    15241 gtgatgagca cgcccgcgcc ataaacaccg gtcggatgaa actgcacgaa ctccatgtcc
    15301 tgcagtggga gccctgcgcg cagcaccatg gcgttgccgt cgcccgtaca ggtgtgggcg
    15361 ctggtacagg agaaataggc gcggccgtaa ccgcctgtcg ccagaatcgt gcggtgtgcg
    15421 cggaaacgat gcagcgtgcc gtcatcgagc ttccaggcca tcacgccgcg gcagacatct
    15481 tcttccatca gaagatcgag ggcgaaatat tcgatgaaga aattcacgtc gtagcgcagc
    15541 gattgtccgt aaagggtatg cagcatcgca tggccggtgc ggtcggcggc ggcgcaggtg
    15601 cgcagcgcct gctccttgcc gtaattcagc gtcatgccgc cgaaggcacg ctggaagatg
    15661 cggccgtcct ccatgcgcga gaacggcacg ccgaaatgtt ccagttcgta gaccgcttcg
    15721 ggcgcgtgac ggcagagata ttcgatcgca tcctggtcgc cgagccaatc ggatcccttg
    15781 accgtgtcgt acatatgcca acgccaatca tcctcgctca tattgccgag cgcggccgaa
    15841 atgccgccct gcgccgccac cgtatggctg cgcgtgggaa ataccttggt gatgcaggca
    15901 accttcagtc cgagcttggc ggcttccagc gtggcgcgca gaccggcgcc acccgcgccc
    15961 accacgacga catcgaatgt gtggtccgtg accggatagg ccgcgctcat caatgcagcg
    16021 ccattttgat caacgcgaaa cccgacgccg cgccgatggc ccaggccgcg aaacgattga
    16081 gcatcaggag ggcgatcttc tggccctcct ggtggatata gtcctcgatg atgatctgca
    16141 gcccgagaga catgtgatag gtgctggcga cgacgaacag gaacatcggc accgcattga
    16201 cgggttcggc gaaatacgcc gccaccgcgc cctgatcggc gccgacatag gcaagcgcgg
    16261 aggccacgaa ccagatggaa agcggcacca gggccaccgc ggtcatgcgt tgatgccaga
    16321 aatgcattgt cccggaccgt ccggcgccga ggccttcgat ccgggagagt ggggtgcgca
    16381 ggcgggcggt catgttccga gccctccgcg aaccatgatc ccgacgacga acgcgcccgc
    16441 cgcaagcagc agcgaaacgc catagacaag cgccgcggtc agtttcgcgg tcggcacttt
    16501 gaagccataa cccacatccc aggcgagatg gcggataccg ttcaggagat ggaaggccag
    16561 cgcccacagg aatccgaaca ggacgacctc gcctaacagg ttggtggcca gccgcatgaa
    16621 aagcccgtag gcatccggcc cggatgcggc tgcgatcagc caccaggcga ggaacagcgt
    16681 gccggcggcc agcgcaatcc ccgtcacccg atgggtgatg gaggccgcca tcgtgatcgt
    16741 ccagcggtag atctgaagat gcggcgacaa aggccgccca aaggggctcg cgcccggccc
    16801 agggctgttg ggggcatcgg ccattccgtt ttccttggtc gagtcgtgaa tttgcgtgtg
    16861 ggccggagtg tagggaggga agggggtaag tcaacggttt caacgccggg aatcggggct
    16921 ttcttagccc ggaatacggg accagacgcc gtccgcgatg cgatagtcgt ttttctgccg
    16981 ctcgcggaag ccgtgcccgt ccgaccattc caggatccgg atcgagaccc cgtctccttc
    17041 gatgtccacc agattgtagg tgttgccgtg accatcgcgc gtgcgggtgg aaatggcggt
    17101 gcctgcgtgc agcaggagaa tggagcgccg ctcggccgtt tccaattcca atgtgccgct
    17161 gagcgggcga tggccgtgac cggacagcag cagatgcaca tccaaccccg cgagcgcctg
    17221 aagcacgtgc ccggaccggc ccgcgattcc atgaggcgtt tcgccggccg gatagccgag
    17281 cggatgatgc gtgaccagcg ccttgaacac gcctcggggc acattcgcga acacgcggcg
    17341 cagatgggcg atctgatcga acgagacgcg gccgctcttg ccgggaaagc gtcttgcggt
    17401 attgagtcca agcaccgcga tgacatcatc ctgaaaaaga tcgccggcga tcccctgggg
    17461 cgcgatatag cggcggaaat tcttgagagg tttgtggagc cggccgatca tatcgtagag
    17521 cggaaggtcg tgattgcccg gcacgaccag tttgggctgc gcgatgttgc cgaggaagcg
    17581 gtgggcctgg gcgaattcgg cctgccgcgc gcgctgcgtg aaatcgccgc tcagcaccac
    17641 cagatcgagc tgttgcaggt tgaggctggc gataaggtcg gcggctatgt gcgtttcatg
    17701 gctgccgaaa tgcagatcgg agagctgggc aattctgcgc atggctcgcg gtctcacgca
    17761 tcgtttcgca cgggcgcgaa aacgcgaagc gcgcgcgggc gaatctcgta atggagcggc
    17821 gggcggagaa gttccacctc accgtcgaag gccaccagca catgatgcct tcgggtttcg
    17881 atgctggcgg tccgcccctt gacgatgcgc atgtcgctta gccgttggga gagcccgaaa
    17941 atcgcgcggc acgccagccg gaaaagcgcc agccggcttt gcgcttttgt gacatacacc
    18001 gacaattccc cgccatccag ccgcgcgcgt ttgccgaggc ccggaagcgt gagctgatag
    18061 tcattgttgc cgatgaacac cagcggagaa cggcaggatt ccgccaatcc ctccacccgc
    18121 acactgagcc ggaacagcgg cagatgacgc agcatgagca ggatggcgag cagcatcgcc
    18181 gatctcttgg ccagatgctg ccggcgtctt tgccgttcgc gttccaggac gagggacgga
    18241 taaaggccga tcgaggaatt gttgaggaaa acctcgccgt tcatcgtgcc gatgtcgacg
    18301 ttccgcaccg cgcgcgtgct catgaccgca acggccgcgc caatgtccaa tggtatcgcg
    18361 agatcgcggg cgaaatgatt gagcgtgcca agcggaagaa tgccgagcgc aacgcctgtg
    18421 tccgccagca cgccggcgac cgtgcggatg ctgccgtctc cgcccccggc cgcaaccgca
    18481 tcgaattgtc ccgcctgcac ctgccgcaac gcctgttcgg ccgcgctgcg caattgcgcg
    18541 ccgggcacca ggctgatggc cgctgtgatc ccggcggcgt cgaaggcggc gcgaagttcc
    18601 tgctcgagct tggccgcgcc cagccgggtc acagtgcctg cgctttcgct gacgagaacg
    18661 gccaagcgca gccgagtggg cgtcgccctc aaggtctggc cggcgaatcg actgggctgg
    18721 gatgttgcag ccatctcatg gccgaccagc acgccagcgc ccatgcccca ccgatgcacc
    18781 agcctgccag cacgtcgctc ggataatgca cgccgagata gaggcggctg aggcccacga
    18841 gaatcgtcag cgccgccgcg agcgccagga tatagacgcg gatcggaatc gaatcatggg
    18901 tttgggcgag aagggcgccg agcgtcagat aggcgatagc cgacagactg gcatggccgc
    18961 tgggaaagct cgtgctgaac acccgcacgc tggcatcgat catgtcaggc cgtggccggg
    19021 aaatcgcgaa tttcagcaga tcgtccaaca ggaccccgcc gatcgccgcg gccaggatcc
    19081 accgcgcgga gccacgcttt cgctccagca ataggtaccc tgtgaccgca agcagcaaga
    19141 gtccgatcac tggaatactg cccaaactcg tgatgtcgcg cgcggtttcc ggtagccagg
    19201 cggggccaat aggaatcgcg cgattgcccg gcacgcgcaa ggcgaggagg atggcgttgt
    19261 cgaaggcgaa gggctgcgct tggattacgt tctcggcgag gacaccgaag acgaacaaaa
    19321 gcgctgcgat ggcggcgagg gccaggaaat tcagctcgcg tgtcctggcc ggggtcattc
    19381 gctcatcctg agacgatccg tgcgtttaga agcagttcgc cggagatgat acaggcaagg
    19441 aacggtcatc ggccaattcc gcaatccagc cgcaatccga gcagcgcctc gagccaagcg
    19501 catacgaacc cgaagctgag acgctgcctg ggacgttggc ctgacgggct ccttcgaaca
    19561 agtttgtgat aataggagac tgggaatttg tgcgggattt gcacagccct ttgacggcgc
    19621 ggaaggtttt tcgccttgtc cctgctgcta gaatcgcgca catagtttcg cccaatggca
    19681 agacctgtcc gcatctctcc ctccatcctg tcggcggatt tcgcgcgtct gggtgaagag
    19741 gtgagggctg tggacgtggc cggtgcggat tttatccata tcgacgtcat ggacggccat
    19801 tttgtcccca atctcaccat cggcccggtc gtggtgaagg cgctcaaggc gcactcccaa
    19861 aaactcttcg actgtcattt gatgatcgcg cccgccgatc cttatgtcgc ggcgtttgcc
    19921 gaagccggcg ccgatatcat cagtattcat cccgaagccg gaccgcatcc tcatcgcacc
    19981 ctgcaattca ttcgttccct ggggaagaaa gccggatatg tgctcaatcc gggcacgccg
    20041 atcgaagtgc tggacccgct cttcgacatg atcgacctga tcctgatcat gacggtcaat
    20101 cccggcttcg gcggtcagca gttccttccc ggtcaattgc agaagataga agccgcgcgc
    20161 aaacgcatcg ccgcgtcagg acgcgaagtg atgctggaag tcgatggcgg catcaatcat
    20221 gaaaccgcga agctcgcgat catggccggc gccgatgtgc tggtcgcggg taccgcgata
    20281 ttccacggcc cagatgacta tgccagcaac atcgctcgcc tccgcggcta agcgcgaggc
    20341 cgctgaggac ggggcgcccg ccgcgccgcg tcccatgcgt gaacgcatgc cgtcggggtg
    20401 ggcgctgtgg atcgaggcgg cgcttgtggc gctgagagct gcgccaatgc cgctcgtgct
    20461 gtggttccgc gagggttggc tctaccgcca gacattgcgc ggtccggtcc ccgaccgcat
    20521 cttgttctat cccagcgaca cgcggatgcg gcggctcgac gatgccgacg gcttcatgcg
    20581 cggccgcttt cgtctgggcg gtcagaagct cgagatccgg cagggttcgg tgttcgatgc
    20641 gccgccgccc ggcgattcgt ttgccgctgc gctgcacggt ttcgattggt tgcgccatct
    20701 cgaagcggcg gggggcgatc tcgcgcgcgg ctttgcgctc aggctcacgc agcaatgggt
    20761 gaagcgttat gcgcgtttca gcccctttgc gtggcgcccg gagatcatcg ccgagcgttt
    20821 cctcaacctc tttgcccatg gccgtttttt cctcgccaat tcggatctgg tctggcgttc
    20881 gaaactattc gtctcgttgc gcaatcaggc ccagatgctc tcgcgcaccg caggcgaagc
    20941 ccccgatggc ctgccccgtc tcaaagcggc cgccgcgctc gcccttgccg gggtctgtct
    21001 cggcgacgtg catcatgaag ccgccgggct gaagcatctc gatggcgaga tcgcgcgcca
    21061 gatcctgccc gatggcgggc acatcagccg ctcgccgcag attctgctcg aagcctatcg
    21121 ggtgctcgcc atggtgcagc aggcgctcga tcatacgagc gcgcggaacg atgcggtgtt
    21181 gcgcggcgcg ctcgacagga tggcgccgat gctgcggttt ttccggatgg gggacggagc
    21241 gctcgccgtt ttccacggcg gcggcgaaag cctgtcgcgg acactggaag cgacgctcga
    21301 cagcgatgac gctttgggcc ggcccttggg ccacgccccc tattcgggat tccagcggct
    21361 ggcggccggc cgtacgctcg tcgtcctgga cgtgggcgca gcgccggaag gcgctttttc
    21421 gacggcagcc catgcgggtt gcctcgccct ggagatgagc gtcggcacca atcggctgat
    21481 cgtcaattgc ggcatggcga tcgacgatag cgaggcgtgg aagacggctt tgcgctcgac
    21541 ggcggctcat tcgaccttaa tgatcgacga tacgtcctcg gcgacgattc ttggggaggg
    21601 gacgctgacg cggtttctgg gtccgcgtct cgtggagggc cctgccggcg tcgagacgga
    21661 gcgcagcgaa agcgcgcaag gcttgaccgt cgcggccggg cacgacggct atcgtgtccc
    21721 ctttggaatc atccaccacc ggcacatgac gctctcccct cgcggcgcgt cgctcaccgg
    21781 tctcgaccgg ctggttcccg ccggtcccga acacgccgcg cgcaatgccg cgcggcggcc
    21841 tttcacgctt cgcttccatg ttcaccccga tgtgcgtttg tcgctggccc aaggcggcgg
    21901 ctccgttatc ctgaagctcc ccaatggcga gggttggcgt tttcgctgca gcggcggcgc
    21961 gctgagcgtc gaggagagca tctatttcgg cgggggcacg gcgcgccggg ccgaacaatt
    22021 ggtggtcagc ggcgcatgga ccggcgaagc gctggagtgc ggctggctgt tcgagcaggt
    22081 gcggtcgtca taggcgcgcg cgcatgatgg aattcctgcg ccggattctg ggtctttccg
    22141 gatcgaaagg cgcgtccttc ccccgtcagt gcaatgtctg tggctatcgt gggcgcttcc
    22201 gggccgcagg caagccccgg cgcatcgatg cgcggtgtcc gcgatgcggc tcggcggagc
    22261 gctaccggct gctggcactt tggctcgacc gccatggcgc cggtcttgcc caagcgaagg
    22321 tgctgcattt cgcgccggaa gcgggcgtca ccgcaatgct gaagacacgc gtcgggcatt
    22381 atgaatccgc cgacattgcg cccggccgcg cggatcgcgt gctgaacatc gaagccatcg
    22441 ccgcccccgg cgccagttat gattgcgtcg tctgctccca tgtcctggag catgtggacg
    22501 acgccaaggc gctccttgag atcatgcgtg tgctgaagcc cggcggcgtc gtgctgatca
    22561 tgctcccggt gatcgagggc tgggcgaaaa cttacgaaaa tcccgccgtg caatcgcccg
    22621 aagaccgcaa gcgccattac ggtcaggccg atcatgtgcg ctattacggt gccgatgtgc
    22681 gcacgcgcat ccggggtgcg ggatttgccc ttaaggaatt caccgccgaa ggccccgacg
    22741 tgctgaccta tgcgctccag cgcggtgaga aagtcttcat cgccacgaag cccgcctgat
    22801 cgccgccatt cggctcattg cttagattag tcctgccgtc ttgcgccgcc ggcgtacagc
    22861 agcaagccca ccgatgccgg caagcaaaag cgcgatcgag gccggttccg gaacggccgc
    22921 ggcggcggcg gcgaagacgc ttctctgccc atcggcggtc aggatgattt cgctcccggc
    22981 agtaaggccc tgaacgttga attgcgacgc cagatcgaat gagggggcaa acgccaatgt
    23041 ccctccgccg gtgaggctga agaagtcatc cagatcgatg gccttgccga aggtgggaac
    23101 gtattcgaag atcaggttga agaccacatc cgttccgatg atcacgttgc ccgagacatt
    23161 gaggtgatcg gcaagaatgt cgctgccgat ttccagattc aatgtgcccg acgccagatc
    23221 gaggttgcca ttgatggttt cggttccggg cgaattgccg ggcgagatgg cgccgccact
    23281 caacaccacg ttggcgtcga ccgtgccgtt gccgttcagc gtgccgtttt gatagatcgt
    23341 caccgtgtcg gcggtcagcg tcgcaccgtt cttcaccgtc aacgtgccgg gtgcggtggt
    23401 ctgcgtgccg ttgaaaccgc cgctgacatg aacattcgcg cctgccgcgt tgacgctcgc
    23461 cgattggtcg accagcaacg tgcccgctgc gaagccgccg atgatgactt catttccagt
    23521 caggagacct gaggtaatcg gtgccaccgt gatgttcgag ccggcacccg ccgttccttc
    23581 tcccgctcgc tgatgaattc ggcgtcgtgg atgatcttcc gatccggatg ggcgacgatg
    23641 tactggacag tcgggtcgtg aaacaccatc gattcgagat aatcgtcgat gaagcgctgt
    23701 tcgaagttga aggtcgcgaa caccggcagg cgcggacgtt cggacccatc gatcacgcaa
    23761 aaagtcgccg cgtggcttcc ggggatctcc acgatctgcc ccatggcctg cggccaaaga
    23821 gtctcgtcga gggccgcgtc gtacagtgcc tccaccgcag agagaatctt gtcgtcgaaa
    23881 ctcaccacac cgtccccccg ggaccgctat gctaatggcg ccaaggcaaa aatgcgagac
    23941 gcgggtgaaa ggcgggcagt ggccaaggcc ggtcgaacgt tgtcagacac gcatgcgctt
    24001 gctattgctg cgttatcgta atttgaaggc tccccccaaa tggttcagtt gaatttgccc
    24061 aaggagagcc gggtcaaacc cggcaagacc tggccgcttg ccaaacgcgt cgacggcaag
    24121 ccgaccaaac gcgccaagcg catccgcatc taccgctacg atccggagat caaggcgaac
    24181 ccgcggctcg acacctacag cgtcgacacg gaagattgtg gaccgatggt tctggacgcg
    24241 ctcatcaaga tcaagaacga ggtcgatcca acgctcacct tccggcgctc ctgccgcgag
    24301 ggcgtctgcg gctcctgcgc catgaacatc gcgggcgcca acacgctcgc ctgcaccaag
    24361 gcgatggccg atgtgtcggg ctccatcaat atctatccgc tgccgcatat gccggtggtc
    24421 aaggatctgg tccccgacat gacggggttc tatgcgcaat acgcgtccat cggaccctat
    24481 ctgcaaacca aatccggcac gccgccgaag gaatggctgc aatcccccga ggaacgcgcg
    24541 aagcttgacg ggctgtacga atgcatcctt tgcgcctgct gttcgacgtc ctgtccgtct
    24601 tattggtgga atccggaacg gtttttaggt ccggccgcgt tgctgcaggc ctatcgctgg
    24661 cttgccgaca gccgcgacga aaacaccggc gacaggctgg atcaccttga agacccgttc
    24721 cgtctctatc gctgccacac catcatgaat tgcgcgaatg tctgccccaa ggggctgaat
    24781 ccttcggaag ccatcgccaa catcaagcag atgatggtga agcgggcggt ttagcgtctg
    24841 tcgtaattct gtccgattat tttcgcagag ccgccacgag accgggaacc ggctggtctg
    24901 cctcgaaacg cagcgtcgtt tgcatggaat cgacgaagga aaggccggcg cgcgcacctt
    24961 cctcccgcag ataggcttcg ctgtgcttaa agcggttttg cggggtttgc tcccaactct
    25021 ctcccgtttt ggattcaacg gcgaagatat agaagccgcc cggattcaaa cgccgggcga
    25081 cgcccgaaaa ggccggcgcg agatcgccga gatacaccat ggtgtcggcc gacaggatga
    25141 ggtcgtagga cagacccgga gcggccagca cgttttccag atcgccgagg atcagatcgt
    25201 catagatccc gcgtgaccgc gcggcttcga tcatgcgtgg cgccaggtcg atgccgtcga
    25261 gccgtccgcc gtccgccgcc acatccttga acgcctcgcc gacgaggccg gtaccgcagc
    25321 caagatcgag gatcgtcaga ggcgctgcaa gcggcacgat cacccgctcg gccaatgtgc
    25381 gcacctgcaa atggccgcga taatggagct tgtccaccat cagcgcgtcg aagcgcggcg
    25441 agatatcgtc gaaggtctcg cgcagggcac gcccatcgat gcggctcacc ttgcgcaatt
    25501 cgagcagcac gtaaaaacga ttctgcttgg ccgtctccag atcgggctgg atggccagcg
    25561 cgcggtcgta gcaggccacc gcttcatcga gctgttgtct ggtgcgcagg acattgccgc
    25621 gattggtcca ggccaccgcg ttttggggat cgagcgccag cacccggtca tatcccgtaa
    25681 gggcctcatc gaggcgcttc atctcgaaca gcgccacagc gcgattgacg agcgcctcga
    25741 tgaagtccgg cttcacggcc aaggcgcgtt cgaaacaatc caatgccgcc gcgtggcgtc
    25801 cgagctgcat cagcgcggcc ccgcgcaggc gaagggcgtc gagcgagacg ggatcgaatc
    25861 gtgccgcttc gctgaagaaa tattgcgcgc gttcgaattg acctgcctga aggcaaaccc
    25921 tgccaaggcc ctcgaaagcc tcgctgcttt tggggtggct gcgcagatgg ccgcgataga
    25981 gcttggtggc ttcctcaagc cgtcccgcct caaaatgatg gtgcgcttcg ctcagggccg
    26041 cgtcggtaat gcgcgatggg gacatggctt aatctgtccc agaatccgcc gcaggaacta
    26101 ttggcttctg cagcgctacg gcaaaacccg gcaccgaagc ggctgcctcc tggcggagcg
    26161 cgcacggcgc gatgtcgacg aacgacagtc ccgcttcggc agcaatgcgc cgcagatagg
    26221 attcgctgtg gcggaagcgg cgcatggggg tctgctccca atcggtctct tgcttgctct
    26281 cgaccgcgaa cagataaaat ccgccgggct ccagccgcgc cgcgacgccc cggaatatcg
    26341 cggccagatc cccgatatag atcatggtgt cggccgcgag aataagatcg taacgcggcc
    26401 caggcgcggg aagaacgctt tccagatcgc ccaggatcag ctcgtcgtaa atcccgcggg
    26461 cgcgggcggc atcgatcatg cgcggcgcga ggtcgatgcc gtcgagacgg ccgccggcgg
    26521 acagatcctt gaagacgtcg ccgaccaggc cggtgccgca tcccagatcg agaatacgcc
    26581 acgcgctttt gtcgcgcggc agaacgcaat tccccaatgt gcgcaggtgc agatgtgcgg
    26641 cataaccgag cttctgaagc atggtctcgt cgtaatgcgg cgcgaaatcg tcgaacagat
    26701 tgcgcatata gcccggtggg cagcggctgg cacgtttcag ctcgaacagc gcattgtcgc
    26761 ggttttcttt cgcctgaggc agatcgggca tcagcgccaa agcgcgctca taactttcga
    26821 tggcctcggc gaaacgcccc atccccgcca gcgtattgcc gcgattgttc cagctgatcg
    26881 cgtggcgcgg atcaagcgca atcgcggcat cgaatccggc cagcgcttcg tccgcgcgat
    26941 tcaattcgag cagcgtcgtg gcgcggctcg acagcgcatc cgcatattgg ggtttcagcg
    27001 acagacagcg gtcgaaggag gacagcgcct gctgatgccg cttcaattgc acaagcacca
    27061 atccgcgcgc gcaccagccc tctgcgaagc cgggattgag catcatggcg ttgctgatca
    27121 gctgctgggc ttcttccaat tggccggtgt gcacggcgat caggcccagc gaatagaacg
    27181 cctcgaactg cgccggatcg gcgaggagaa ttttctggta gagatcggcg gcctctttca
    27241 gccgtccggc ctggtgcaga ccccgcgcgc gttgcatgag aatttcgttc atggcgcggc
    27301 caagacctat tttctgagcg cgatggtgaa gcccgcaacc gcggcgccgg tctcgaagcg
    27361 caaggcgctt tccacgcgat ccacgaaggc caagccggca cggccggcgc attcgcggac
    27421 ataggattcg ctgtggcgga aacggcgctt cggcgtcggt tcccagccgt cgccgtcctt
    27481 gctctcgacg gtgaagatga agaatccacc cggcagcaaa tgcgcggagg caccctgcag
    27541 aatcgcgttc agatcgccga aatagatcag cgtgtcggcg gcgatcatca gatcgtagat
    27601 ttcaccgccg gtgccgagca tctgttcgat ctcgccgaga tagagattgt tgtagatgcc
    27661 gcgcttgcgg gcctcatcca gcatccgtgg agaaaaatcg atgccgtcaa ggcgcccgcc
    27721 gccggtggtc caggcgtgga actgatcccc ggccaggccc gtaccgcagc ccagatcgag
    27781 cacccgcaat ccgaatcgcg catcgggcca cacccggtcc gccatctcgc gcaattgcag
    27841 atggccgcga taattcaaat tgttgagcag cgaatcttcg aacagcagcg cgttgtcatc
    27901 gaacaggcgg cgataggctt gccggtaggc gatggtgtgc aagccgtgcg cttcggggta
    27961 atcgggcctt agcttgagcg cctcttcgaa gcgtgtgagt gcttcgccga attgttcgcg
    28021 gcgcatcata tcctcgcctt gctgacacag aatttccgcg aagcgcagcc cgccgcgatt
    28081 catctcaccg agcgcgagca gccggttctg ccgcgcatcg ggaaaatccg gatagatcgc
    28141 gagcgcgcgg tcgtagctct cgatcgcctc gccatagcgc ttgagcgcga acagcgcgtt
    28201 gccgcgattg ttccaactca gcacatggcc gggatcgagc accagcaccc ggtccagccc
    28261 ggcgagacct tcgtgcaggc ggcccaactc cagcagcgtc gtggcctgat tgctcagggc
    28321 ctcgatgaaa tcgggtttca cgccgagcgc ctgatcgaaa gaggcgagcg cttcgtcggc
    28381 acgcgaaagc ttcatcagcg cgaccccacg catgcacaga gcgtcggcga aggagggatc
    28441 gcacctgacc gcttcgccga aaagatattc ggcctgatcg aattggcttt gctggaaata
    28501 gatcatccca agcgcatagc gcgcggggcc atggacggga ttggcgttca ggaaatcatg
    28561 gtagagccgg gcggcgtcgg cgagccggcc ttcctgggaa gcctgttgtg cgtctagcag
    28621 cagcgggtcg gacatggggg cggaatgtgt ctcagaatga ccgccgccgc cagcatcttc
    28681 gtgagggatc agaccggttt gaagcggccg gcgtctgcgc cgccgcgcag cgacaacaag
    28741 cgccataaga gggcgcttgc catccggctg agccgtccag agggattgag gccggtcggt
    28801 ttcaccacca tcttcaccag ccggtcgatg tgatggcggc gatagaacgc catgatgcgt
    28861 tgctgatagg ccctggcata tatacgcctg cgatagggct tggcgtccgc ctcggcaggc
    28921 gaaacattgg cacaataata ggcacaggca agctcgtcat catccgattc attaacccgg
    28981 gtgaccgcca cccaaagctt gcgccacaaa ggaaggtcgg gctccgtctc gtcctgcagg
    29041 atgtcgtaga acaggcggta atggcggaat tcgtcggcgg cgatgcgccc cgccacttcc
    29101 tgcagcaccg gctcgtcgct ggcatcgcga atggcgctgt agagcgatga ggtcccgctt
    29161 tccaccacgc agcgcgcgat catctcgccg cgcctggacc cgcgcaccga gcccttgccg
    29221 ccgctgaaat gcgccgcctg gtacagcgac cggaaacggg tgaaggcggt gatgaaatcg
    29281 aagcaaggat cggccaattc ggcccagcgc gccagagcga gaccatgctg gatctcttct
    29341 tcgccccaat gttcgatgct gcgaagggtt gattcagggg tgccgctata gacccgcttc
    29401 agataggtca catagtccgg tgcgttgtat tccacgagcg acgccgcctt cacggcagcc
    29461 aacagcgctg gcttcacctt ggaagggtcg aaacgggacc aatcgacatc gtcgagcgtc
    29521 cagcccgcct cgtaaaccac gccatcggtc atc
//
LOCUS       DASYEG010000150        11620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1644025, whole genome shotgun sequence.
ACCESSION   DASYEG010000150 DASYEG010000000
VERSION     DASYEG010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 11620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11620
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644025"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VGM72_06805"
     CDS             <1..973
                     /locus_tag="VGM72_06805"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4265010.1"
                     /translation="RHRGLAPGFCFRIGHCMKLAVPDIISNSYFPAAAAVELGFFRDE
                     GLDVSLELIFPVDKAYAALRDGAVDFVGGSAHSALAAFPEWQGAKLLCAQAQGMYWFL
                     VMHAGLNAARGDISAVKGKSIGAAPWVEMGLRRLLSEAGIDLARDDVRIAPVPGAVGA
                     GVNFGVTAARALEERRIDGFWANGMGAEVAVRRGIGTIVLDVRRGDGPKACFNYTMSS
                     LAASDALIARAPDVAAAAVRAVVKTQAALRADPERAAEVGRKLFPPAEAALIAELIRR
                     DLPFYDASISPEFVGGMNQFARDVGILRGDPAYEDVVATQFRDLWRE"
     gene            complement(1000..1965)
                     /locus_tag="VGM72_06810"
     CDS             complement(1000..1965)
                     /locus_tag="VGM72_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265011.1"
                     /translation="MKTSIVVALALLVIGIGVWALWDRIAVMAAPKKEAIAIRSEAAI
                     KADDLFWTTFHNAQYGNIESTLEALTAAYLQTPSDAKTAAHIAWSHNWRMAERARMQS
                     PSATITDDTMLARRYFQEAAKLDPSDARVLGFLAGHTMIEGTLHKDERLTRKGYFMML
                     DAIKAWPEFNMFTGGYVFSRLPADSPQFREGLDWQWQTLDKCIGGKLDRANPDYSAYM
                     AKETREGPKRACWNSWIAPHNLEGFFLNMGDMLVKAGDWQTARKIYADAKLSKSYPSW
                     KFQSVLEDRIDRAQANVALFNAPDSTPVTQIMIDSKFACSACHQQ"
     gene            2316..3764
                     /locus_tag="VGM72_06815"
     CDS             2316..3764
                     /locus_tag="VGM72_06815"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HEY4265012.1"
                     /translation="MRNGQFLCAVFAAVIATPGAMATPLLLATGTLTGSSAGSNQDLS
                     GLTGTLENGLPANILGGLGSGIAYAGGNIFLAVPDRGPNATPFDPAIDDTVSFISRVQ
                     TLTLQLTPNSGVLPFSLGATLDATTLLYSPTPLVYGSGAGLGNKIDGVTPIGSGAPAQ
                     NTAGAFYFTGRSDNFDPAGNSGNPADARFDPESIRVAPDGKSVFVSDEYGPYVYQFDR
                     QTGARIKSFALPANLDVANLSPVGATEISGNTSGRTANKGMEGLAITPDGKTLVGIMQ
                     AALIQDAATPASKNLLRIVTIDIATGVTHEYGYLLTAGSGVSDIVAINDHEFLIDERD
                     GAGLGDNSSAAVKQVFRIDLAGAVDITGLTGAAAAAEAVGKTLVLDAVSVLNANGFTD
                     TNIPSKLEGLALGPDVMLDGTLEHTLFFTNDNDFVPGVAGPNQFFVFGLTDADLPGFQ
                     QEAFVPEPSTLALFGAGFLGLFAFGMMRRRAG"
     gene            complement(3761..4729)
                     /gene="egtD"
                     /locus_tag="VGM72_06820"
     CDS             complement(3761..4729)
                     /gene="egtD"
                     /locus_tag="VGM72_06820"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEY4265013.1"
                     /translation="MNPRTGFATSIPPREPALAEEDFADALLAGLRSTPKRIACKYFY
                     DAAGSLLFEKICTLPEYYPTRTELGLLRAHAHEMAEKIRPDAEIVEFGAGAGEKIRPL
                     LHALENPRIYLPIDISGPYLRTIATRMQSEYPHLTIKPIVGDFTRDVSLPPASGPRVG
                     FFPGSTIGNFEPGEAQAFLAKAAKLLKGGGLLIGVDLVKDPNLLHAAYNDRAGVTAAF
                     NKNLLVRANREAGANFELSHFAHYALYNPNLRRMEMYLVSQATQRVTICGRSISFAQG
                     EAIHTEYSHKYTIEDFHALASAAGFVPRAVWCDPGRLFSIHWLEAA"
     gene            complement(4726..6003)
                     /gene="egtB"
                     /locus_tag="VGM72_06825"
     CDS             complement(4726..6003)
                     /gene="egtB"
                     /locus_tag="VGM72_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEY4265014.1"
                     /translation="MYRTLASDPPDSNTQFSARYNSIRILTDRLTAPLSAEDQCIQSM
                     PDASPVKWHLAHTTWFFETLVLMPHLKGYEPYDPRYHYLFNSYYEALGPRHPRPQRGL
                     LTRPALDEIRNYRRHVDGAMGRLFEHARQAERLEFEDLAELGLHHEQQHQELILTDIK
                     HAFSCNPLLPAYLALTSRAVKKAPPLEWIDCAGGILPIGHSQDGFAFDNEGPRHLAVL
                     TPYRIASRLITCGEYLAFMDDGGYRRPEFWLSDGWAMARAQDWTAPLYWAKIDEEWQI
                     FTLNGVQPMDRDEPVVHVSFYEAAAYASWAGKRLPTESEWEAVAADVPVTGNLLDPAR
                     PHPRPASADGKIAQLYGDAWEWTRSSYDPYPGFKPLSGAVAEYNGKFMCGQMVLRGGS
                     CVTPPGHIRSSYRNFFPPSARWQFSGIRLAEDA"
     gene            complement(6157..9051)
                     /locus_tag="VGM72_06830"
     CDS             complement(6157..9051)
                     /locus_tag="VGM72_06830"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HEY4265015.1"
                     /translation="MNPLQTLHGFGQAIWLDFLSRRFVAEAGLKRLVQEDDLTGVTSN
                     PSIFKKAIAGSSDYDSSLDAAVKDSDCDVMTLYETLAIADIRNAADVLAPVYRDTKRA
                     DGFVSLEVSPYLANDTQATIAEARRLWKAVGRENVMIKVPATEAGIPAIRQLLGEGIN
                     INITLLFARAMYRQVAQAYLDGMETFLAGGGDATRLASVASFFVSRIDTAVDRLINEK
                     LKQLPAGDADTLGPLRGKIAIANAKLAYQDYKQLFAGPRWEALKQKGARVQRLLWAST
                     SAKNPAYRDVVYVEELIGPDTVNTVPPETLDAFRDHGQVRMSLEENVETADSMLAMLD
                     RFGIALDSVTTKLIEEGVQLFAEAFDDLLAAVQKKRAAQLGSALNRQSVTLPPTMDEA
                     VKTALEEWRKDGKVRRLWRRDAAVWTGSDEAKWLGWLDIAAQESADIGRLNRIAEHAR
                     DMRFTHAVLLGMGGSSLGAEVFANTFGPVSGGPVSGGPISGGAAPGHPKLLVLDSTDP
                     EEIKSIEDAIDPKQTLFIVSSKSGSTLEPNILKDYFFARAEAALGKGNAGSHFIAITD
                     PGSKLEATARDERFRQICFGKPDIGGRYSVLSDFGLVPAAAMGVDVKSLLSSAETMVY
                     SCGAHVPPGENPGVRLGAVLGVLGRQGRDKITIIASPAIADIGAWLEQLLAESTGKHG
                     LGLVPLDGEPLGPPDVYGKDRLFVYVRLDNAPDKAQDDSVSALEKIGQPVVRIALGER
                     IGIGQEFFRWEMATAVAGAIIGINPFDQPDVEASKIKTRALMAAYEQGGTPPPETPLF
                     EENGIRIFADSTNAAALNAPGTTTLAGVLRAHFARLKPDDYCGVLAYVARNDRHQTLL
                     QNIRVAVRDAKHVATCLGFGPRFLHSTGQAYKGGPNSGVFLQITAGHPRDLAVPGRNY
                     SFGAVIDATAQGDFAVLNERGRRAIRVDLGTDAEAGLARLGEAVKTALAE"
     gene            9209..10885
                     /gene="pgm"
                     /locus_tag="VGM72_06835"
     CDS             9209..10885
                     /gene="pgm"
                     /locus_tag="VGM72_06835"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HEY4265016.1"
                     /translation="MAGKISPLAGKPVTSGMLVNVPRLMTAYFTLVPDAAIAAERIAF
                     GTSGHRGSAFDTAFNEAHILAVAQAICLYRQQNGIDGPHFVGMDTHALSEAALTSGIE
                     VMAANGVTTMLDARSGYTPTPLISHAVLAYNRGRTGGFADGVVITPSHNPPEDGGFKY
                     NTPNGGPADVAATDWIQKTANRFLADGLKGVKRMTYARARKEPCFHVHDYIAAYVADL
                     DNVVDMEAIRSAGVKIGIDPLGGASVHVWEPLIERYGINASVVNQEVDPTFRFMTADW
                     DGKIRMDCSSPYAMMRLVAMRDEFDIAFANDTDADRHGIVCRSSGLMNPNYYLTAAIS
                     YLFRNRPGWRDDLSIGKTVVSSGMIDRAAGQVHRKLIEVPVGFKWFVDGLIGGTMGFG
                     GEESAGASFLRRDGTVWTTDKDGLILGLLAAEITAKTGRDPGELYNGLTHDFGPSFYE
                     RIDATATPQQKTILKNLTPEKLDAKELAGEPVEAMISNAPGNNAPIGGIKVSAKSGWF
                     AARPSGTEDVYKIYAESFRSAEHLKQIQENAQTLVSRSFAKEPDSKEPGG"
     gene            10896..>11620
                     /locus_tag="VGM72_06840"
     CDS             10896..>11620
                     /locus_tag="VGM72_06840"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY4265017.1"
                     /translation="MSLDGVLLSRLQFAFTIGYHILWPAYTIGISGFIVVLNVLWLLT
                     RKQVYRDQLRFWIHLFALGFAMGVVTGVVLSYEIGANWSVFAERTSNVLAPFFGYEVL
                     TAFFLEAGFIGIMLFGMDRFGPRLHMLSCMVVAFGAVASAFWILAANSWMQTPAGFRM
                     EGGKFQIVSWWAAVFTPSFPYRFFHMITAAYLTGTFVVIGVMGWYLWRRQHLAFARAG
                     FSVAMGIAAVLAPLQLAIGDAHGL"
BASE COUNT         2161 a         3457 c         3613 g         2389 t
ORIGIN      
        1 tcggcatcgc ggcctggctc cgggattttg tttcaggatc ggacattgca tgaagctcgc
       61 ggtccccgac atcatctcca attcctattt tcctgccgca gcggccgtcg agctggggtt
      121 cttccgcgat gagggtctcg acgtctcgct cgaattgatc ttcccggtcg acaaggccta
      181 tgcggccttg cgcgacggcg cggtcgattt tgtcggcggc tcggcgcatt cggcgctggc
      241 cgcattcccg gaatggcagg gcgcgaaact cctctgtgcg caggcacaag gcatgtactg
      301 gttcctggtc atgcacgccg gcctgaacgc ggcgcgcggc gacatctcgg cggtgaaggg
      361 caagagcatt ggtgcggccc cctgggtgga gatggggttg cggcgattgc tgagcgaagc
      421 cggaatcgac ctcgcgcggg atgatgtgcg aatcgcgcct gtgcccggcg cggtgggagc
      481 gggcgtcaat ttcggcgtca ccgcggcgcg cgcgctggaa gaacgcagga tcgatggctt
      541 ctgggcgaac ggcatgggcg cggaggtggc ggtgcgccgc ggcatcggca cgatagttct
      601 ggacgtgcgg cgcggcgacg gtcccaaggc gtgcttcaat tacaccatgt cctcgctggc
      661 cgcgagcgat gcgctgatcg cgcgcgcgcc cgatgtggcg gccgcggccg tgcgcgcggt
      721 cgtcaagacg caggcggcgt tgcgcgccga tcccgaacgc gcggccgaag tggggcgcaa
      781 actgtttcca ccggcggagg cggctctgat cgcggagctg atacggcgcg atcttccatt
      841 ctatgacgcg tcgatttcac cggagttcgt tggcggcatg aatcaattcg cgcgcgatgt
      901 cggaatcctc agaggcgatc ctgcctatga ggatgtcgtc gcgacgcaat ttcgtgattt
      961 gtggagggaa tagcgggtca atcctcagtc tctcatgcgc tattgctgat ggcaggctga
     1021 gcaagcgaat ttcgagtcga tcatgatctg cgtcacagga gtgctgtccg gcgcattgaa
     1081 caacgcgacg ttggcttgcg cgcgatcgat gcgatcctcc agaaccgatt ggaatttcca
     1141 actcggatat gattttgaga gcttggcatc cgcgtagatc ttccgcgccg tttgccagtc
     1201 gcccgctttg acgagcatgt cccccatatt caggaaaaat ccctccagat tgtgcggcgc
     1261 gatccacgaa ttccagcagg cccgctttgg gccctccctt gtttccttcg ccatatacgc
     1321 agaatagtcc gggtttgcgc gatcgagctt gccgccgatg cacttgtcca gcgtttgcca
     1381 ctgccaatcg agaccttccc ggaattgcgg tgaatcggcc ggcagccggc tgaagacata
     1441 tccgccggtg aacatattga attccggcca tgccttgatt gcgtcgagca tcatgaaata
     1501 gcccttgcgg gtcagccgct catctttgtg cagcgtgccc tcgatcatgg tgtgcccggc
     1561 cagaaacccg agaacgcgag catccgaggg atcgagtttc gccgcttcct gaaaatatct
     1621 ccgcgccagc atcgtgtcgt ccgtgatggt agccgacggc gattgcatcc gggcgcgttc
     1681 ggccattcgc caattgtgcg accaggcgat gtgagctgct gttttcgcat cgctgggtgt
     1741 ctgtagatag gcggcggtca gcgcttcaag ggtgctctcg atattgccgt attgtgcatt
     1801 gtggaacgtg gtccagaaca gatcgtcggc cttgattgcc gcctcgctcc ggatagcaat
     1861 cgcctctttc tttggagcgg ccatcacggc gatccgatcc cataaggccc acacgccaat
     1921 gccgatgacc agcaacgcca gggcgacgac aatacttgtt ttcatagacc tgatctcaga
     1981 tggcctgtgc actgacgatg gggacgctgc atcgctcccc gatagcggcg aacgtgtgat
     2041 gcgcgggaac gttccatcaa ttcgcgcaca gacgatggtg tgacggtgcc ttcaattgcg
     2101 gatctcttcg ccatttccag gcacgatgat cgcttcgggc ggtttcgcga ttttcggcag
     2161 aggcaagtga cattcagctg tcacagtctt gatccagatt gcatgcgctg gggcctcaga
     2221 cggaggttat tcggcggccg cctgagtgct gaaaacagtc gctgctgcat tttcgcacac
     2281 atcggcatga ccatcatcat ctggggagcc gatgcatgcg taacggacaa tttctttgtg
     2341 cggtctttgc ggccgtaatc gcgacacctg gcgcaatggc cacaccgctt cttctggcaa
     2401 caggcacgct gaccggcagt tcggcaggat cgaaccagga tctttcgggt ctgaccggta
     2461 cgctcgaaaa cggcttgcct gcaaatattt taggagggct ggggtccggt atcgcctatg
     2521 cgggcggcaa tatttttctt gccgtgccgg accggggccc caacgcaacc ccgttcgatc
     2581 cggcgatcga cgacaccgtc tcgttcattt cgcgcgttca aaccctgacg ctgcaactga
     2641 cgccgaacag cggggtcttg ccattttcgc ttggcgcgac tctggatgcg acgaccctgt
     2701 tgtacagccc aacgccgctc gtttatggca gcggtgccgg actcggcaat aagatagatg
     2761 gcgttacgcc tatcggttcg ggcgcgcccg cgcaaaacac cgccggcgca ttctatttta
     2821 cgggacgttc agacaatttc gatccggccg gcaattcggg aaatccggcc gatgcgcgct
     2881 tcgatccgga atcgattcgc gtggcgcctg acggcaagag cgtcttcgtt tccgacgaat
     2941 acgggcccta tgtgtatcaa ttcgaccgcc agaccggcgc acgcatcaag agttttgcgc
     3001 tccccgccaa cctcgacgtc gccaatcttt cgcctgtggg agcgacggaa atcagcggca
     3061 acacatccgg ccgcacggcg aacaagggca tggaagggct tgccatcacg cccgatggca
     3121 aaacccttgt cggcatcatg caggcggcgc tgattcagga cgcggcgaca ccggcctcga
     3181 agaatcttct gcggatcgtc acaatcgata tcgcgaccgg cgtaactcat gaatatgggt
     3241 atctgctgac ggctgggtcg ggcgtaagcg atattgtcgc gatcaacgat catgaatttc
     3301 tgatcgatga gcgtgacggg gcaggtctcg gcgacaatag ctccgcggcg gtgaaacagg
     3361 tcttcaggat cgacctcgcc ggcgccgtcg atatcactgg cttgacaggc gcggcagcgg
     3421 ccgccgaagc ggtcggcaaa acccttgtcc tcgacgccgt gagcgtcctc aacgcgaacg
     3481 ggttcaccga taccaatatt ccatccaagc tcgaaggtct cgcacttggc cccgacgtga
     3541 tgctggatgg aaccctggaa cacacgctct ttttcacgaa tgacaatgat ttcgtgccgg
     3601 gcgttgcggg gccgaaccag ttcttcgtat tcggcctgac ggatgcggac ctgccgggct
     3661 ttcagcaaga agcgttcgtt ccagaaccct ctacgcttgc cctcttcgga gccggcttcc
     3721 tgggtctctt cgcgttcggc atgatgcgca gacgcgcagg ctaggcagct tccagccaat
     3781 ggatgctgaa gagccggccg ggatcgcacc acaccgcgcg tgggacgaac ccggccgctg
     3841 aggccagcgc gtggaaatcc tcgatcgtat atttgtgtga atattcggtg tggatcgctt
     3901 cgccttgcgc gaaagaaatg ctgcggccgc aaatcgtgac gcgctgcgtc gcttggctca
     3961 ccagatacat ctccatgcgg cggagattcg gattgtagag cgcgtaatgc gcaaaatgcg
     4021 acagttcgaa attcgcgccc gcttcgcgat tggcgcgcac cagcagattc ttgttgaacg
     4081 ccgcggtgac tccggcgcga tcattgtacg ccgcgtgcag caggttcggg tctttcacca
     4141 gatcgacgcc gatcaggagc ccgccgcctt tcagcagctt tgcggccttg gcgaggaacg
     4201 cctgcgcttc gccgggttcg aaattcccta ttgtcgagcc cggaaagaaa ccgacgcggg
     4261 gtccgctcgc gggcggcagc gaaacatcgc gcgtgaagtc gccgacaatc ggcttgatgg
     4321 tcaggtgcgg atattcgctt tgcatccgcg tcgcgatggt tcgcagatag gggccggaga
     4381 tatcgatggg cagataaatg cggggatttt ccaatgcatg gagcagcggc ctgatcttct
     4441 cgcccgcacc ggccccgaat tccacgatct ccgcatccgg ccggatcttc tcggccatct
     4501 catgggcgtg ggcgcgaagc aggccaagct ccgtgcgcgt cggataatat tcgggcagcg
     4561 tgcaaatctt ctcgaacagc agcgagcccg ccgcgtcgta gaaatatttg caggctattc
     4621 gcttgggtgt gcttcgcagg cccgcgagca gggcatcggc gaaatcttct tcggcgagcg
     4681 cgggctcgcg cggcggaatg cttgtagcga agcccgtgcg cgggttcatg catcctccgc
     4741 gaggcggatg ccggagaatt gccagcgggc ggacggtgga aagaaattgc ggtagctgga
     4801 acggatatgg ccgggcggcg tgacgcagga gccgccgcgc agcaccatct gcccacacat
     4861 gaatttgccg ttgtattcgg ccacagcgcc ggatagcggt ttgaatccgg gataggggtc
     4921 gtagctcgat cgcgtccatt cccaggcatc gccatacaat tgcgcgattt ttccatccgc
     4981 gctcgcgggc ctgggatgcg gccgtgcggg atcgagcaga tttcccgtga ccggcacatc
     5041 ggccgcgacg gcttcccatt ccgattccgt cggcaggcgc ttgcccgccc aggacgcata
     5101 ggcggcggcc tcgtagaaac tgacatgcac caccggctcg tcacgatcca tcggctggac
     5161 gccgttcaag gtgaagatct gccattcctc atcgattttc gcccaataaa gcggtgcggt
     5221 ccaatcctgc gcccgcgcca tagcccagcc gtccgacaac cagaattcgg ggcggcgata
     5281 cccgccgtcg tccatgaagg cgagatattc gccgcatgtg atcaggcgcg aagcaatccg
     5341 gtaaggcgtc agcacggcca gatgccgcgg gccttcattg tcgaacgcaa atccgtcctg
     5401 cgaatggccg atgggcagaa tgccgcccgc gcaatcgatc cattcgaggg gcggcgcttt
     5461 tttcaccgcg cgcgaggtga gggccagata ggcgggcagc agcggattgc aagagaatgc
     5521 atgcttgata tcggtgagga tcagctcctg atgctgctgc tcatgatgca gcccaagctc
     5581 ggccagatcc tcgaactcga gccgttcggc ctgtctggcg tgctcgaaaa gacgtcccat
     5641 cgcgccatcc acatggcgcc ggtaattgcg tatttcgtcc agcgccggac gtgtcagaag
     5701 gccgcgctgg ggtcttggat ggcgcgggcc cagcgcttcg taataggaat tgaacagata
     5761 atgatagcgc gggtcgtaag gctcatagcc cttgagatgc ggcatcaaca ccagggtttc
     5821 gaaaaaccat gtggtatggg caagatgcca tttgaccggg ctcgcatccg gcatcgattg
     5881 gatgcactga tcctccgcgc tcaagggcgc ggtaagccga tccgtcagta tacggatgga
     5941 attataacgg gctgagaact gggtattgct gtccggcggg tcgctggcaa gagtgcgata
     6001 catgaggctc gaacctgctc cgtaacaaaa atataccgcc cagccatggc ctgacagctt
     6061 ttccatggcc tgacagcttt tgagcgcgta ttaaacgcac cgaacacgta tttgtgccag
     6121 caccgttctg gctttaccgt atttaggacg cgcggctcat tccgcaaggg cggttttcac
     6181 ggcttctccc aatcgggcga gcccggcttc ggcatcggtt cccagatcga cacggatcgc
     6241 acgccgcccg cgctcgttga gcaccgcgaa atcgccttgt gcggttgcgt cgatgaccgc
     6301 accaaaactg tagtttcggc ccgggaccgc aagatcgcgc ggatgaccgg cggtgatctg
     6361 caggaagaca ccgctgttgg gaccgccctt gtaggcttgg cccgtggaat gcaggaagcg
     6421 cggcccaaag ccgaggcagg tcgcgacatg tttggcatcg cgcaccgcca cgcggatgtt
     6481 ctgcaacagg gtttggtggc ggtcgttgcg cgcgacgtaa gcgagaacgc cgcaataatc
     6541 gtccggcttc aggcgcgcga aatgcgcccg cagcactccg gccaaagtcg tggttcccgg
     6601 cgcgttcagc gcggcggcat tggtcgaatc cgcaaaaatc cggatgccgt tttcttcgaa
     6661 caatggcgtt tccggcggtg gggtcccgcc ttgttcatag gcggccatca gcgcccgggt
     6721 cttgatcttg ctcgcttcga catcgggctg gtcgaacgga ttgatcccga tgatggcgcc
     6781 ggcgacggcg gttgccatct cccaccggaa gaattcttgg ccgattccaa tgcgttcgcc
     6841 aagcgcgatg cgcaccaccg gctggccgat tttttccagc gccgaaacgc tatcgtcctg
     6901 ggctttgtcc ggcgcattgt cgagccggac atagacgaac agccggtcct tgccataaac
     6961 atccggcggc cccagcggtt cgccgtcgag gggaaccagg cccagcccat gcttgccggt
     7021 ggattcggcg agcaattgtt cgagccaggc gccgatatcg gcaatggccg gagaggcgat
     7081 gatcgtgatc ttgtcgcgtc cctgccgccc gagcacgccc agcacggcac cgagtctgac
     7141 accgggattt tcccccggcg gcacatgggc cccgcaggaa tagaccatcg tctccgcgga
     7201 ggagaggagg gatttcacgt ccacgcccat cgcggcggcg ggcaccagcc cgaaatcgga
     7261 cagcaccgaa tagcgtccgc cgatatcggg cttgccgaaa cagatttgac ggaaacgctc
     7321 gtcgcgcgcc gtcgcttcca gcttggaacc gggatcggtg atggcgatga aatgcgatcc
     7381 ggcattgcct ttgcccagcg cggcttcggc gcgagcgaag aaataatcct tgaggatatt
     7441 gggttcgagc gtgctgcccg atttgctcga tacgatgaac agcgtctgct tcggatcgat
     7501 ggcgtcttcg atgctcttga tttcttcggg atcggtggaa tcgagcacca gcaatttcgg
     7561 atgaccgggc gccgcaccgc cagaaattgg accaccggaa actgggccac cggaaactgg
     7621 gccaaacgtg ttggcgaaca cttccgcgcc caggctcgat ccccccatgc cgagcagaac
     7681 cgcatgggtg aagcgcatat cgcgcgcgtg ctccgcgatt ctgttcagac ggccgatatc
     7741 ggcgctttcc tgcgcggcga tatcgagcca tcccagccat ttcgcttcat cgctgccggt
     7801 ccataccgcg gcatcgcgcc gccacaggcg gcggaccttg ccgtccttgc gccattcttc
     7861 gagtgcggtc ttcaccgcct catccatcgt gggcggcagg gtgacgcttt gccggttgag
     7921 tgcggagcca agctgcgcgg cgcgcttctt ttgaacggcg gcaagaagat cgtcaaacgc
     7981 ctccgcaaaa agctgcacgc cttcttcgat caatttcgtg gtcaccgaat cgagcgcgat
     8041 tccgaaacgg tcgagcatcg caagcatgct gtcggcggtt tcgacatttt cttccaggct
     8101 catccgcacc tgaccatgat cgcggaaggc gtccagggtt tccggcggca ccgtgttgac
     8161 ggtgtccggc ccgatcagct cctcgacata gacgacatcg cgataggcgg gattcttggc
     8221 gctggtgctc gcccagagca ggcgctgcac ccgcgcgcct ttctgtttga gagcctccca
     8281 gcgcgggccg gcgaaaagct gcttgtaatc ctgatacgcg agtttggcat tggcgatggc
     8341 gatcttgccg cgcaacggcc ccagcgtgtc cgcgtcgccg gccggcaatt gcttcagttt
     8401 ttcgttgatc agacggtcga cggccgtgtc gatgcggctg acgaagaagc tcgcgacgct
     8461 ggccagacgc gtggcgtcgc cgcccccggc gaggaatgtc tccatgccgt cgagataggc
     8521 ttgcgcgacc tggcgataca tcgcgcgtgc gaacagcagc gtgatgttga tgttgatgcc
     8581 ttcgccgaga agctggcgga tcgcgggaat gccggcctcg gtcgccggca ccttgatcat
     8641 gacattctcg cgtccgaccg ctttccacag acgccgtgcc tcggcgatcg tggcctgcgt
     8701 atcgttcgcg agatagggag agacttcgag actgacgaaa ccatcggccc gctttgtatc
     8761 gcggtacacc ggcgccagca cgtcggcggc gttacggatg tccgcgatgg cgagcgtttc
     8821 gtacagagtc atgacgtcgc aatcggaatc ctttaccgcc gcatccaggg acgaatcgta
     8881 atcgctgctg ccggcgatgg cctttttgaa gatggaggga ttggaggtga cgcccgtaag
     8941 atcgtcctcc tggacgagcc gtttcaaccc ggcctccgcg acgaagcggc gcgacaggaa
     9001 atcaagccat atggcttgac caaatccatg cagggtctga agagggttca cgtgatgatc
     9061 ctcaaggaag cgggtctcat aaagcgaagg acgtcattgc atcgctttaa gcccctctta
     9121 gcaatggggc aaatttgcaa tgatacgttt gggaaccatc cttttggcaa ggcgttgaga
     9181 tgtattcacc tggaaccatg agtcagcagt ggccggcaag ataagtccgc tcgcgggaaa
     9241 acccgttacc tccggcatgc tggtgaatgt accgcgcctg atgacggcct atttcacgct
     9301 ggtgcccgat gcggcgatag cagccgagcg gatcgccttc ggcacgtcgg gacaccgggg
     9361 ttcggctttc gataccgcct tcaacgaggc ccatatcctg gcggtcgcgc aggcaatttg
     9421 cctttatcgg cagcagaacg gaatcgatgg gcctcacttc gtcggcatgg acacgcatgc
     9481 gctgtcggaa gcggcattga cgagcgggat tgaagtcatg gccgccaacg gcgtcaccac
     9541 catgctggac gcgcgaagcg gctatacgcc gacgccgctg atctcgcacg cggtgctcgc
     9601 ctataatcgc ggccgcacgg gagggttcgc cgacggggtc gtcatcactc cgtcgcacaa
     9661 tccgcccgag gatggcggct tcaaatataa tacgccgaac ggcggcccgg ctgatgtggc
     9721 ggcgaccgat tggattcaga agaccgccaa tcgattcctg gccgatgggc tcaaaggcgt
     9781 gaaacgcatg acctatgcgc gggcgcgcaa ggagccttgc ttccacgtcc acgattatat
     9841 tgccgcctat gtggccgatc tggacaatgt ggtcgatatg gaggccatcc gaagcgcggg
     9901 cgtcaaaatc gggatcgatc ccttgggcgg agcgagcgtt cacgtctggg agccgctcat
     9961 tgagcgatac ggcatcaatg cgagcgtcgt gaaccaagag gtcgacccga cttttcgttt
    10021 catgacggcc gattgggatg gcaagatccg catggattgc tcatcgcctt acgcgatgat
    10081 gcggctggtc gccatgcgcg acgaattcga tatcgctttc gcgaacgaca cggatgcgga
    10141 tcgccatggc atcgtgtgcc ggtcgagcgg cttgatgaat cccaattatt acctcacggc
    10201 cgcgatttcc tatctgttcc gcaatcgtcc gggctggcgg gacgacctgt ctatcggcaa
    10261 gacggtggtt tcaagcggca tgatcgaccg cgccgcgggc caggtccatc gcaaattgat
    10321 cgaagtgccg gtcggtttca aatggttcgt agacggattg atcggcggca cgatgggatt
    10381 cggcggcgaa gaaagcgcag gcgcgtcttt cctgcgccgc gacggtacgg tgtggaccac
    10441 cgacaaggac ggtctcattc tgggccttct tgccgccgag atcaccgcga agacgggacg
    10501 cgatccgggt gaactctaca atggcctgac gcacgatttt ggcccctcct tctatgagcg
    10561 gatcgatgcc accgcgacgc cacagcagaa gacgatcctg aaaaatctga ccccggaaaa
    10621 attggacgcg aaggagcttg ccggtgagcc ggtcgaggcc atgatttcca acgcgccggg
    10681 caacaacgcg cccatcggcg gcatcaaagt gtccgccaag agcggctggt tcgcggcgcg
    10741 gccatccgga accgaggatg tctataaaat ctatgccgag agcttccgca gcgccgagca
    10801 tctgaagcag atccaagaga atgcgcaaac cctggtgtcc cgcagcttcg ccaaggagcc
    10861 cgattccaag gagcctggcg gctagaggtt ttaccatgag cctcgatggg gtgttgctgt
    10921 cgcgcctgca attcgccttt accatcggct atcacattct gtggccggcc tatacgattg
    10981 gcatctccgg tttcatcgtc gtgctgaatg tgctgtggct tctcacgcgc aaacaggtct
    11041 atcgcgacca attgcgtttc tggattcatc ttttcgcgct cggcttcgcc atgggcgtgg
    11101 tgacgggtgt ggtcctgtcc tacgagatcg gcgccaattg gagcgtcttc gccgaacgca
    11161 ccagcaatgt gctggcaccg tttttcggtt acgaggtgct gacggcgttt tttctcgagg
    11221 cgggcttcat cggcatcatg ctgttcggca tggaccgctt tgggccacgg ctgcatatgc
    11281 tttcctgcat ggtggtcgca ttcggcgcgg tggcgagcgc gttctggatt ctcgcggcga
    11341 atagctggat gcagacaccg gccggctttc gcatggaggg cggaaaattc cagatcgtct
    11401 cctggtgggc ggcggttttc acgccgtcct ttccttaccg tttcttccac atgattacgg
    11461 cggcctatct cacgggcacc ttcgtcgtga tcggcgtgat gggttggtat ctctggcggc
    11521 ggcaacatct ggcgtttgcc cgtgcgggct tttcggtggc aatgggaatc gccgccgtgc
    11581 tggcgccgct gcagttggca atcggcgacg cgcatgggct
//
LOCUS       DASYEG010000151        14051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_5122447, whole genome shotgun sequence.
ACCESSION   DASYEG010000151 DASYEG010000000
VERSION     DASYEG010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 14051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14051
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5122447"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VGM72_06845"
     CDS             <1..237
                     /locus_tag="VGM72_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265018.1"
                     /translation="LEGPLASRAEQAFLAGCDIVLHCNGNLDEMRDVAREAKPLAGPA
                     LQRAEAAMAQLRAPLKFDIEGAETRLTELLGSNA"
     gene            234..1058
                     /locus_tag="VGM72_06850"
     CDS             234..1058
                     /locus_tag="VGM72_06850"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEY4265019.1"
                     /translation="MNEPELTGIENFQSTPVAVEDALIIEVDGFEGPLDLLLALARNQ
                     KVDIAKISVLQLADQYLVFMETARRHRLELAADYLVMAAWLTFLKSRLVLPQPAGPEA
                     AGAEEMAAQLRWRLTRLQMMRDASVRLLARDRLGRDVFGRGQPEPILVVRTRKHADTM
                     FDLLTAYSQQRVRTLGHRVYEMARAPVFLIEEARARIERQLGRISDWSALTRFLPLDW
                     GMGARRRTSLASTFSAALELTRDGRLELRQLTPFGDIFLKDRTTAAAAAVAGEGTN"
     gene            1055..1813
                     /gene="scpB"
                     /locus_tag="VGM72_06855"
     CDS             1055..1813
                     /gene="scpB"
                     /locus_tag="VGM72_06855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEY4265020.1"
                     /translation="MNRIIRLSEAEMAEPAELDIENPTVPEGESQCVLDPMHLRIVEA
                     LLFASTEPVEEPALAKALPQGADIPAILVELQQHYQGRGVNLIKVAGKWQMRTANDLA
                     FLLRKQSVEERKLSRAAIETLAIIAYHQPVTRAEIEDIRGVMVSKGTIDALMEAGWVR
                     IRGRRRTPGRPVTYGTTEAFLTHFGLESVGDLPGMDELKAAGFLDAVPPSSFSVPNPS
                     DALAPDEDPYEGDEEEPRDGDGLPDPVDHLPAEE"
     gene            1826..3073
                     /locus_tag="VGM72_06860"
     CDS             1826..3073
                     /locus_tag="VGM72_06860"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HEY4265021.1"
                     /translation="MSSVESPGSTPQHGSVWFVLIAVFIDMVGVGIAIPVLPVLIGAY
                     TASREMQAYWSMALLAAYGLMQFLCAPMLGAISDRFGRRPVLIAAIIGLGLHYFLIAI
                     APTLLFMLVARLIGGMTGASFSVANAYLADITPPDQRARNFGLVGAAFGLGFICGPMI
                     GGILGGIDLHLPFYAAAGISALNAVYGYLIVPESLPLERRTKFSLGRANPFRALIALI
                     RHHAVGGLVLVYAFYFIAHMTMVQSFVLATHFRFGWGTFENGLLLFCVGLLSTIVQGG
                     LIGRLVKRFGEERLVLMAVGTNVIIQTLYGLATQGWMIFPILFAGFLIFTAGPSIQAV
                     VSKSADPGTQGVTMGALQSISSLAAVIGPLLGNALLAQVADLPPGDIRLGANFFACAL
                     LNLLAFAIAWRRLVKPAGAAVSH"
     gene            3073..3648
                     /locus_tag="VGM72_06865"
     CDS             3073..3648
                     /locus_tag="VGM72_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016393899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEY4265022.1"
                     /translation="MEALLVSTGVVALAEIGDKTQLLAILLAVRFRTPVPIILGILCA
                     TIFNHTLAAILGTYIGAWLGQNLSGILAVSFLAMGLWTLVPDKLDKEPRLFENFGAFG
                     ATLISFFLIEMGDKTQLATMALAARFNNIALVASGTTLGMMIADVPAVYLGDILANKI
                     PLRLVRMAAAAIFILLGLAAALNWGRRFVWA"
     gene            complement(3645..4835)
                     /gene="rnd"
                     /locus_tag="VGM72_06870"
     CDS             complement(3645..4835)
                     /gene="rnd"
                     /locus_tag="VGM72_06870"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEY4265023.1"
                     /translation="MRIITKTAELKALAAELEHGPYVAIDTEFMRDQTYWPKLCLIQA
                     AAPGIEAIIDPLAEGIDLTPFHGLLATKSVVKVFHAARQDVEIFHHQGGVIPNPLFDT
                     QIAAMVCGFGDSASYETLVRKLAKADIDKSARFTDWSRRPLSKRQLEYALADVTYLRL
                     VYELLSKELEKTGRGKWVEEEEATLTDPATYRLDPSEAWRRLKARSGNKRFLQVLAGV
                     AAWREREAQTRDVPRNRILKDEALLEIASHPPPDAEHLGQIRALPNGYANSRAGKALM
                     TALKEALAGPPPPEFMLPPERRPRRSEPTPAAMDLLKTLLRLRATQYRVAPRLVANSE
                     DLERLAAGESDGVPALHGWRAEVFGNDAIALREGRLAIALENGEAVVMETGAEKKPRA
                     RRGA"
     gene            5065..6867
                     /gene="aspS"
                     /locus_tag="VGM72_06875"
     CDS             5065..6867
                     /gene="aspS"
                     /locus_tag="VGM72_06875"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEY4265024.1"
                     /translation="MHIYRTHHCGELRRSDVGARVRLSGWVHRSRDHGGLLFIDLRDH
                     YGLTQCVIEPDSPAFAVASQARSEWVLTVTGPVVARTPDTINPDLPTGEIELRIEEVT
                     IQSAAEELPLPVFGDLDYPEETRLRYRFLDLRRERLHKNIVLRSNVVQSIRRRMVAEG
                     FTEFQTPILTASSPEGARDFLVPSRLHAGKFYALPQAPQQFKQLIMVAGFDRYFQIAP
                     CFRDEDGRADRSPGEFYQLDIEMSFVTQNDVFAAIEPVLHGVFEEFSNGRKISPMPFP
                     RIPYSEAMLLYGSDKPDLRNPILIADVKAIFDRDDVSFKAFKGKEAVRAIPAPGAASQ
                     PRSWFDKMNEWARGEGAAGLGYIVFEEENGMLSGKGPIAKFLPAEALRQLAVTAAAKP
                     GDAVFFAADKTQRAATLAGQARLRIGNELGLTRKDCFEFCWIVDFPMYEWNEDEKRID
                     FSHNPFSMPNFDSEKFLVLDPKDRETILGITAFQYDIVCNGVELSSGAIRNHKPETML
                     KAFEIAGYARSETESRFGGMLNAFRYGAPPHGGTAPGIDRIVMLLADVPNIREVTMFP
                     MNQKAEDLLMGAPSEVSAKQLRELHIRVVLPEKA"
     gene            6929..7303
                     /locus_tag="VGM72_06880"
     CDS             6929..7303
                     /locus_tag="VGM72_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265025.1"
                     /translation="MLTIGTALGAAQYGWWRYMTIAAHQASATGTVLATNCRNNNDVS
                     YSFSVQGKTLQGRDSWIDCKALYRGDTLPISFSSLDPAQNMAGDGYARFVSETISILL
                     ASVLGSIVVAAVFVYPFGKRRS"
     gene            complement(7300..8421)
                     /locus_tag="VGM72_06885"
     CDS             complement(7300..8421)
                     /locus_tag="VGM72_06885"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005085.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEY4265026.1"
                     /translation="MAAPKPPFRADHVGSLLRPPALHEARAKAQKGEITHDALRGIED
                     AAIRDAVALQQACGLEAITDGEFRRGHYLVDFLTGFEGIAPTNTSYALAFKGEHGESG
                     ETRSMLTVTHKVRRAKPIMVEGFKFLKSLTKHTPKLCLPSPTWIHMRGGRKMVNESAY
                     PDIEEFWDDIVRAFHEEMRDLAAAGCTYLQLDEISFAFLCDATIAARMRADGLDPETE
                     TRNYARVVNAIAAGAPPSMTVTVHTCRGNFQSMWMAEGGYERVASIVFDQPDVDGYFL
                     EYDSERSGGFEPLQYVPKDKKIVLGLVSSKKPDIESKDALKRRIDEAAKFFPIEQMCL
                     SPQCGFASTHHGNKITEDVERRKLALIVETAAEIWGSAV"
     gene            complement(8441..8932)
                     /locus_tag="VGM72_06890"
     CDS             complement(8441..8932)
                     /locus_tag="VGM72_06890"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit B is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP F0F1 synthase subunit B"
                     /protein_id="HEY4265027.1"
                     /translation="MELLRDPEFWVGIGTLVFLALLLWKRVPGLVISSLDARAAAIAK
                     ELEEARRLRSDAEAFLAEYKKKHAAADEEASSIVAEAKAEALRFAAESRSAIKAQIER
                     RGRQAEEKIAQAEAQAVSELRALAADAAIAAAEKLIAARLDDNQSAQLVKWSLGEIPS
                     KLN"
     gene            complement(8934..9479)
                     /locus_tag="VGM72_06895"
     CDS             complement(8934..9479)
                     /locus_tag="VGM72_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265028.1"
                     /translation="MAAPQETTEGTAAEVKVGLPQMKTDTFPSQIFWLTVTFAILFVV
                     MWRMTLPMIAGAIGARRNRIEDDLSTAEGFRKDAAEALASYEAALAQARGRAHQLADE
                     NRKRVVAEIEQLKTAADTETQATIATAEKRIASERVKAVTGVRASAADAAADIVQRLI
                     GRSVSAEEAAAAIASAEPRGR"
     gene            complement(9488..9712)
                     /locus_tag="VGM72_06900"
     CDS             complement(9488..9712)
                     /locus_tag="VGM72_06900"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEY4265029.1"
                     /translation="MDLASAKMIGAGLASIALIGAGVGIGIIFGNYLAGALRNPGAAQ
                     GQFTNLLVGFALCEFTGLLGVVMAFIILFT"
     gene            complement(9766..10518)
                     /locus_tag="VGM72_06905"
     CDS             complement(9766..10518)
                     /locus_tag="VGM72_06905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004482.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY4265030.1"
                     /translation="MASPMEQFEVRPIVPLHVGNLDISFTNISLWMCIVVAAAILFLT
                     AGASSRRLVPTRMQSITEMSYVFVADMIRSAAGTEGLKFFPFVFTLFMFVLFSNVIGL
                     FPLIHPFTVTSHIIVTFSLAIFVFLMVIVVGLARHGLHFFSIFAPKVPLPMLILLVPI
                     EIVSFLSRPISLSVRLFANMLAGHTMLYVFGTFVIGLAAAGGALSVISIAPFLAIVAV
                     AALEFLIAFLQAYVFAILTCIYLNDALNLHEH"
     gene            complement(10536..10847)
                     /locus_tag="VGM72_06910"
     CDS             complement(10536..10847)
                     /locus_tag="VGM72_06910"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEY4265031.1"
                     /translation="MAEDDPDARRLAELQERLQKARGKETPRPQEGSQSQLGIAFRLV
                     ADLLAGVIVGGGIGWALDRVFGTSPFLLIVMFLLGVAAGMRNLMRTAREMNQRQDRSG
                     G"
     gene            complement(11047..11553)
                     /locus_tag="VGM72_06915"
     CDS             complement(11047..11553)
                     /locus_tag="VGM72_06915"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY4265032.1"
                     /translation="MDIVVRREGALYVAEWGIGQRRCAVGQSGIGEKLREGDGLTPMG
                     CWPLRRVLYRPDRMEAPATGLAVAIISPDDAWCDVPDDPNYNRLVKLPYPSGDERLWR
                     EDSLYDLIVVVGFNDSPVLPGRGSAIFLHVARPDYEPTQGCVAFLRDDLIAAVAQLTP
                     MDRLIVTP"
     gene            complement(11574..12068)
                     /locus_tag="VGM72_06920"
     CDS             complement(11574..12068)
                     /locus_tag="VGM72_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265033.1"
                     /translation="MPPLSDIRTATSGISSSPVASANAAKPTQGLTFGDLLDTINPLQ
                     HIPIIGTIYRAITGDVMSPTAEVAGGALFGGIAGAVASLADVVFTQATGKDFGATVLS
                     WLGLEKGVAGKTQFAQAGNSALSSDGPARTSDPAIVARANEAYESAISLQDALPAARS
                     SFRY"
     gene            complement(12222..13382)
                     /gene="ribA"
                     /locus_tag="VGM72_06925"
     CDS             complement(12222..13382)
                     /gene="ribA"
                     /locus_tag="VGM72_06925"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEY4265034.1"
                     /translation="MTADKPGEIKPVISQALIPPEQRVERAAGDLRRGAPVLIRADAA
                     GESGIAVAAETVKSETLVALGGVSGPSALILTHARATTLKIRLYTADIVSIALPSRLN
                     AADIRAIADPATDLDHPLKGPFEASRGQPPQSAGAAVRLAKLAGLLPAAVLFPIPDNA
                     RTPLQIAGALSEVSVADIHGFERRAAETLQLVVRASVPLAGAEQAELAAFRPENGGPE
                     HYAVIIGAKGAPALSSPGPVLTRLHSECFTGDFLASLKCDCGDQLRGAIQRIAKEGGG
                     ILLYLAQEGRGIGLMNKLRAYRLQDEGFDTIEANQRLGFEADERLYDIAACMLRLLGY
                     SKVRLLTNNPGKVAALEAAGIEVTMRVPHAFPENAHNRDYLRVKAEKSGHIF"
     gene            13465..>14051
                     /locus_tag="VGM72_06930"
     CDS             13465..>14051
                     /locus_tag="VGM72_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4265035.1"
                     /translation="MPSTKRVLLVDDDEMLRASLAEQLEADGTYEPVEAGTCREGQQK
                     GLEGLYEFMVLDVSLPDGDGRELCRAFRDSGVTCPIIILTASDSDADTIHGLQAGAND
                     YITKPFRFSVLMARVDAHLRSHGASEEAIYKIGPYTFRPSAKVLLEGGKRIRLTEKET
                     NILKYLQRAGSTVSRDVLLHEVWGYNPAVSTHTLET"
BASE COUNT         2589 a         4265 c         4378 g         2819 t
ORIGIN      
        1 ctcgaagggc cgctggcgtc gcgcgccgaa caggccttcc tcgcgggttg cgacatcgtg
       61 cttcactgca acggcaatct cgatgagatg agggacgtcg cgcgggaagc caagccgctt
      121 gccggtccgg cgctgcaacg cgccgaagcg gcgatggcgc aattgcgcgc gcctctgaaa
      181 ttcgacatcg aaggcgccga aacgcggctg accgaattgc tgggttcgaa cgcatgaatg
      241 agcccgaact caccgggatc gagaatttcc aaagcacgcc ggtcgcggtg gaagatgcgc
      301 tgatcatcga ggttgatggc ttcgagggac cgctcgacct tttgctcgcg ctcgcgcgca
      361 accaaaaagt ggatatcgcg aagatttccg tcctccagct cgctgaccaa tatctggttt
      421 tcatggagac agcgcgccgt caccggctcg aacttgccgc cgattatctg gtgatggccg
      481 catggctcac cttcctcaaa tcccgcctgg tcctgccgca gcccgccggt cccgaagccg
      541 ccggtgccga agaaatggcg gcccagctgc gctggcggct gacgcggctg cagatgatgc
      601 gcgacgcatc ggtgcgattg ctggcccgcg accggcttgg ccgcgatgtc ttcggccggg
      661 gacagcccga accgatcctg gtcgtgcgca cccgcaagca cgccgatacg atgttcgatc
      721 tgctcaccgc ctattcgcaa caacgtgtgc gcacgctcgg ccatcgcgtc tatgagatgg
      781 cgcgcgcgcc ggtctttctc atcgaagaag cccgcgcgcg cattgagcgc cagctcggca
      841 ggatttcgga ctggagcgcg ctgacgcgct tccttccgct cgattggggg atgggtgcgc
      901 ggcggcgcac ctcgcttgcc agcacgtttt cggcggcgct cgagttgacc cgcgatggcc
      961 ggctcgaatt gcgccagttg acaccgttcg gagacatttt cctgaaggac cgcacgacgg
     1021 ctgccgcggc ggccgtggcc ggggagggca caaattgaac cgcatcatcc gtctcagcga
     1081 ggctgaaatg gccgaacctg ccgagctcga tatcgagaat cccacagtgc ccgagggcga
     1141 atcgcaatgt gtgctcgatc cgatgcattt gcgcattgtc gaagcgctgc tgtttgcatc
     1201 gaccgaaccg gtcgaggaac ccgctttggc gaaggcgttg ccgcaaggcg ccgatattcc
     1261 tgcgatcctc gtggagttgc agcagcatta ccaggggcgc ggggtcaacc tgatcaaggt
     1321 cgccggcaaa tggcagatgc gtaccgccaa cgatctggct ttccttctgc gcaagcaatc
     1381 ggtggaggaa cgcaagcttt cgcgtgcggc catcgagacg cttgcgatca ttgcctatca
     1441 tcagcccgtc acgcgtgcgg agatcgaaga catacgcggc gtgatggtga gcaagggcac
     1501 tatcgatgcg ctgatggaag ccggctgggt acggatcaga gggcgcagac gcacgccggg
     1561 gcgtcccgtg acctatggaa cgacggaagc cttcctgacg cattttggcc ttgagagcgt
     1621 tggcgatctt ccgggaatgg acgagttgaa ggccgcgggc ttcctcgatg cggttccacc
     1681 atccagcttt tctgtgccca atccctccga tgccctcgcc cccgacgaag atccttatga
     1741 aggcgacgaa gaggagccac gcgacgggga cggccttccc gacccggtgg accatctgcc
     1801 ggccgaagaa tagacatagt ttccgatgtc cagcgtcgag tcccctggtt ccacgccgca
     1861 gcatggcagc gtctggttcg ttcttattgc cgtcttcatc gacatggtgg gggtcggcat
     1921 tgccattccg gtgctgccgg tcctgatcgg tgcttacaca gcctcgcgcg aaatgcaggc
     1981 ctattggagc atggccctgc ttgccgccta tgggctcatg cagtttctgt gcgcgcccat
     2041 gctgggtgct atcagtgacc gcttcggccg gcggcccgtc ctcatcgccg ccattatcgg
     2101 cctgggcctg cactatttcc tgatcgcgat tgcgcccacg ctgctgttca tgctggtggc
     2161 gcggctgatc ggtggcatga ccggcgcaag tttttcggtg gcgaacgcct atctcgccga
     2221 tatcacgccg ccggatcaac gcgcgagaaa tttcggactg gtcggcgccg cgttcggcct
     2281 gggcttcatc tgcggaccaa tgatcggcgg cattctgggc ggcatcgatc tgcatttgcc
     2341 cttttatgcc gccgcgggca tttcggctct gaacgcggtc tatggttatc tgatcgttcc
     2401 cgaatccctg ccgctcgaac ggcggaccaa attttcgctt ggccgggcca atccgttccg
     2461 cgcgctgatc gcgctgatcc gtcatcacgc cgtgggtggg cttgtgctcg tctacgcgtt
     2521 ttatttcatc gcccatatga cgatggtgca gagcttcgtg cttgccacgc atttccgctt
     2581 cggctgggga acgttcgaga atggccttct gctgttttgt gtcgggcttt tatcgaccat
     2641 cgtgcagggc gggctcatcg gccggctggt aaagcgcttc ggcgaggaac gccttgtgct
     2701 gatggcggtc gggaccaatg tcatcatcca gacgctttat ggactcgcga cgcaaggctg
     2761 gatgatattc ccgatcctgt tcgcgggctt tctcatcttc acggcggggc cgtccatcca
     2821 ggcggtggtt tcgaaatcgg ccgatcctgg cacgcaaggt gtgacgatgg gcgctctgca
     2881 atccatcagc agtcttgccg cggtgatcgg accgttgctc ggaaacgcgc ttctggccca
     2941 agtcgccgac cttccccccg gcgacatcag actcggtgcg aatttcttcg cctgcgcgct
     3001 tctcaatttg cttgcatttg cgatcgcttg gcgccggctc gtaaagcccg ccggtgccgc
     3061 ggtttctcat tgatggaagc gctccttgtt tcgacgggcg tcgttgcctt ggcggaaatc
     3121 ggcgacaaga cgcaacttct cgccatcctg ctggccgtgc ggtttcgtac gcccgtgccg
     3181 atcatcctgg ggatattgtg cgcgacgatt ttcaaccaca cgctcgccgc gatcctggga
     3241 acctacatag gcgcgtggct gggtcaaaat ctgagcggga ttctggcggt gtcgtttctc
     3301 gccatgggat tgtggacgct ggtgcccgac aaactcgata aagagcccag gctgttcgaa
     3361 aatttcggcg ccttcggggc gacgctgatc tcattcttcc tgatcgaaat gggcgacaag
     3421 acgcagcttg ccaccatggc gctcgccgcc cggttcaaca atatcgcgct ggtggccagc
     3481 ggaaccacac tcggcatgat gatcgcggat gtgccggcgg tttatctcgg cgatattctc
     3541 gccaacaaaa ttccgctccg gcttgtacgc atggccgcgg cggcgatttt tatattgctg
     3601 ggacttgcgg cagcgttgaa ctggggcagg cgttttgtct gggcctaggc gccccgcctg
     3661 gcacgcggct ttttttctgc gccggtttcc atgaccaccg cttccccatt ttcaagcgcg
     3721 atcgcgaggc gtccttcacg cagggctatg gcgtcgttac cgaacacttc tgcgcgccag
     3781 ccatgcagcg cgggtacgcc gtcgctttcg cccgccgcaa gccgctcgag atcctcggag
     3841 ttcgccacca gccgcggcgc gacccggtat tgcgtcgccc gcaggcgcag cagcgtcttg
     3901 aggagatcca tggcggcggg ggtcggttcg gaacggcgcg ggcgccgctc gggcggcagc
     3961 atgaattcgg gggggggggg accggccagc gcctccttga gcgcggtcat cagggccttg
     4021 cccgcgcgcg aattcgcgta gccattgggt aacgcccgga tctggcccaa atgctcggcg
     4081 tcgggcggcg gatgggatgc gatctccagc aacgcctcat ccttgagaat gcgattccgg
     4141 ggcacatcgc gggtttgcgc ttcgcgttcg cgccaggcgg ccacgccggc aagcacctgc
     4201 aggaaacgtt tgttgcccga gcgcgccttg aggcgccgcc aggcttcgga gggatcaagc
     4261 cgataggtgg cggggtctgt cagagtggct tcttcctctt ccacccattt gccgcggccg
     4321 gtcttctcca attccttcga cagcagttca tagaccaggc gcagataggt cacatcggcc
     4381 agcgcatatt cgagttggcg cttggacagc ggccgccggc tccaatcggt aaagcgggcg
     4441 gatttgtcga tgtccgcctt ggcgagcttg cgcacgagcg tctcatagga ggccgaatcg
     4501 ccgaatccgc acaccatggc ggcaatctgg gtgtcgaaca gcggattggg gatgacgccg
     4561 ccctgatgat ggaagatttc cacatcctgg cgtgccgcgt gaaacacttt caccacggat
     4621 ttcgtggcca gaagaccgtg aaacggggtg agatcgatcc cctccgcgag ggggtcgatg
     4681 atggcttcga tgccaggggc cgcggcctgg atcaagcaca gtttgggcca ataggtctga
     4741 tcgcgcatga attcggtgtc gatggccaca tacggaccat gttccagctc tgcggcgagt
     4801 gccttcaact ccgccgtttt ggtgatgatg cgcatgaagg ggtcttagcg gattcgccct
     4861 tggcctgtcg ctgttttcct gtgctcaaaa gggccgtaaa atgagaatct taagggcata
     4921 cgcccttgaa cccgcaggat tggccgccct tcggcatgtc cttggggaat ccggttgcgg
     4981 tcgccgcaaa tctcttcggt tttcgaaccg ccttgacttc ctgaagcggg ctttccacaa
     5041 acgccggccc ggacgaggaa ccccatgcac atctaccgca cccatcattg cggcgaactg
     5101 cgccgatcgg acgtcggtgc gcgcgtgcgc ctttctggct gggtgcaccg gtcccgcgat
     5161 catggcggat tgctgttcat cgacttgcgg gatcattacg gcctgacgca atgcgtgatc
     5221 gagccagaca gcccggcgtt tgccgtcgcc tcccaggcgc gctcggaatg ggtgctgacc
     5281 gtgaccggac cggtcgtggc ccgcacgccc gacaccatca atcccgattt gccgaccggc
     5341 gagatcgagc tgcgcatcga ggaagtgact atccaaagcg cggccgaaga attgccactg
     5401 cccgtgttcg gcgatctcga ttatcccgaa gagacgcggc tgcgttaccg cttcctggat
     5461 ttgcgccgtg agcggctgca caagaacatc gtgttgagat cgaacgtcgt gcagtccatc
     5521 cgccggcgga tggtggccga aggtttcacc gaattccaga ccccgatcct gaccgcatcc
     5581 agcccggaag gcgcgcgcga cttcctggtg ccgtcacgcc tgcatgcggg aaaattctac
     5641 gcgctgcccc aggcgccgca gcaattcaaa cagcttatca tggtggcggg cttcgaccgc
     5701 tatttccaga tcgcgccatg ctttcgcgat gaggatggcc gcgcggatcg cagtcccggc
     5761 gagttctatc agctcgatat cgagatgagc ttcgtcaccc agaacgatgt gttcgcggcg
     5821 atcgagccgg tgcttcatgg cgtgttcgag gaattctcga atggccgtaa aatttcaccc
     5881 atgccgttcc cacgcattcc ctattctgag gcgatgctgc tttacggctc cgacaagccc
     5941 gatctgcgca acccgatcct gatcgcggat gtgaaggcga ttttcgaccg cgacgatgtc
     6001 tcgttcaaag ccttcaaagg caaagaagcc gtgcgcgcga ttccggcgcc cggcgcggct
     6061 tcgcagcctc gctcctggtt cgacaagatg aacgaatggg cgcgcggcga aggcgctgcc
     6121 ggccttggct acatcgtgtt cgaggaagag aacgggatgc tctccggcaa ggggcccatc
     6181 gcgaaattcc tgccggccga agcgctccgg caattggctg tcacggctgc cgccaagccg
     6241 ggagacgcgg tgttcttcgc ggccgacaag acgcaacgtg cggccacgct cgcgggccag
     6301 gcgcggctga gaattggcaa tgaattgggg ctgacgcgaa aggattgttt cgagttctgc
     6361 tggatcgtcg attttccgat gtatgaatgg aacgaggacg agaagagaat cgatttctcg
     6421 cacaatccgt tctccatgcc gaatttcgat tccgagaaat tcctggttct cgatcccaag
     6481 gaccgcgaga cgatcctcgg gatcacggcg ttccaatatg acatcgtgtg caacggcgtg
     6541 gaattgtcgt cgggcgcgat ccgcaaccac aagccggaaa ccatgttgaa ggcgttcgag
     6601 atcgcgggtt acgcgcgcag cgaaacggaa agccgcttcg gcggcatgct gaatgcgttc
     6661 cgctatggcg cgccgcctca tggcggcacg gcgccgggca tagaccggat cgtgatgctc
     6721 ctcgccgacg tgccgaacat ccgtgaagtc acgatgttcc cgatgaatca aaaggccgag
     6781 gacttgctga tgggggcgcc ctccgaggtt tcggccaagc aattgcgcga gttgcatatc
     6841 cgcgtggttt tgccggaaaa agcgtaagtc tacgcgctca atcaggagaa cgcacacgaa
     6901 gcgcggaagc tacatcggac ttgcagcggt gctcaccatc ggcaccgcgc ttggcgcggc
     6961 ccaatatggc tggtggcgct atatgaccat cgcggcgcat caggcgagcg cgaccggtac
     7021 ggtgctcgcc accaattgca ggaacaacaa cgatgtttcc tattcttttt cggtacaagg
     7081 aaaaaccctg caaggacgcg acagctggat cgactgcaag gccctgtatc ggggagatac
     7141 gttgccgata tcgttttcct cgcttgatcc ggcccagaat atggcaggag acggttacgc
     7201 gcgtttcgtg agcgagacga tttcgatttt gctggcctcg gtcctcggct ccatagtggt
     7261 cgcggcggtt ttcgtttacc cgttcggtaa acggcgcagc tagaccgccg agccccagat
     7321 ctcggcggcg gtttccacga tcagcgcgag cttgcggcgc tcgacatcct cggtgatctt
     7381 gttgccgtga tgggtgctgg caaagccgca ttgcggcgac aggcacattt gttcgatggg
     7441 aaagaatttc gccgcctcat cgatccgccg cttcagcgca tccttggatt cgatgtccgg
     7501 tttcttcgaa ctgacaaggc cgagcacgat tttcttgtcc ttcgggacgt attgcagcgg
     7561 ctcgaatccg cccgagcgtt cgctgtcata ttccaggaaa tagccgtcga catcgggctg
     7621 gtcgaagaca atggacgcca cacgctcata gccgccttcg gccatccaca tgctctggaa
     7681 attgccacgg caggtatgga cggtcactgt catggaaggc ggtgcgccgg ccgcgatggc
     7741 attcaccacg cgcgcataat tgcgtgtctc ggtctcggga tcgagaccat cggcgcgcat
     7801 gcgcgcggcg atcgtcgcat cgcaaagaaa cgcgaagctg atttcatcga gctgaagata
     7861 ggtgcagccc gcggcggcca ggtcgcgcat ctcctcatgg aaggcacgga cgatatcgtc
     7921 ccagaattcc tcgatgtcgg gataggcgct ttcattcacc attttgcgcc cgccccgcat
     7981 gtggatccag gtgggtgagg gcaggcacag tttcggcgtg tgcttggtca aggatttgag
     8041 gaatttgaac ccttccacca tgatgggttt cgcgcggcgt accttgtgcg tcaccgtcag
     8101 catggagcgg gtttcgcccg attcgccatg ttcgcccttg aaggcgaggg catagctcgt
     8161 gttcgtgggc gcgatgcctt cgaacccggt caggaaatcc accagataat ggccacggcg
     8221 gaattcgccg tcggtgatcg cctcaaggcc gcacgcttgc tgaagcgcaa ccgcgtcgcg
     8281 aatggcggca tcttcgatac cgcgcagcgc atcatgcgtg atttcgcctt tttgcgcttt
     8341 cgcgcgcgcc tcatgcagcg cgggcgggcg cagcaggctt cctacgtgat cggctctgaa
     8401 gggaggtttc ggcgcggcca ttttggttcc gcttggcatg tcagttcagc ttggagggga
     8461 tttctccgag cgaccacttt accagttggg ccgactgatt gtcatccaag cgcgccgcga
     8521 tcagcttttc cgctgctgct attgcggcat cggcggcaag cgcacgcaat tccgagacgg
     8581 cttgcgcttc ggcctgagcg atcttttctt ccgcttgccg gccccgccgt tcgatctgag
     8641 cctttatcgc actgcgcgat tccgctgcaa agcgcagcgc ttcggctttg gcttccgcga
     8701 cgatggagct tgcttcctca tcggcggcgg catgtttctt cttatattcg gcaaggaacg
     8761 cttcggcatc gctacgcagg cggcgggcct cttcgagttc cttggcgatg gcggctgcgc
     8821 gcgcgtcgag ggaactgatc acaagccccg gcacacgctt ccagagcagc aaagccagaa
     8881 acaccagcgt gccgataccg acccagaatt cgggatcgcg aaggagttcc atgtcagcgt
     8941 cccctcggct cggccgatgc gattgctgcc gcagcctcct cggcgctcac gctgcggccg
     9001 atcagtcttt gcacgatatc ggcggcggcg tccgcggcac tggctctgac gccggtcacg
     9061 gctttgacgc gttccgacgc gatgcgcttt tcggcggtcg caatcgtggc ttgggtctcg
     9121 gtatcagccg cggtcttcag ctgctcgatc tccgcgacga cgcgcttgcg attctcgtcg
     9181 gcgagttgat gcgcgcggcc acgggcttgc gcaagcgcgg cttcgtagga ggcaagcgct
     9241 tcggcggcat ccttgcgaaa gccttcggcg gttgaaagat cgtcttcgat ccgattgcgc
     9301 cgcgcaccga tcgctccggc gatcatgggc agggtcatgc gccacatcac gacgaacaga
     9361 attgcgaacg tcaccgtcag ccagaatatc tggctgggga aggtgtccgt cttcatctgc
     9421 ggcaggccga ccttgacctc ggccgcagtc ccttccgtcg tttcttgtgg cgcggccatg
     9481 aggcctctca ggtgaagaga atgataaagg ccatcaccac gcccagaagg ccggtgaatt
     9541 cgcacagcgc gaagccgacg agcagattgg tgaattggcc ttgtgccgcg ccgggattgc
     9601 gcagcgcgcc tgccagataa ttgccgaaga tgatgccgat gcccacgccc gcgccgatga
     9661 gcgcgatgga ggccaggcca gcgccgatca tttttgccga tgcaagatcc atatttgaac
     9721 tcctttgttt cgtttgcaag cgctgttgcg cgccagtcct tgggtttagt gctcgtgcag
     9781 attgagcgcg tcgttgagat agatgcaggt gagtatcgcg aagacatagg cttgcaggaa
     9841 tgcgatcagg aattcgagtg cggcgaccgc gacgatcgcc aaaaacggcg cgattgagat
     9901 caccgacaga gcgccgcccg cggccgcaag gccgatcacg aatgtgccga acacatagag
     9961 catcgtgtgc ccggccagca tgttggcgaa caaacggacg gaaaggctga tcggccggga
    10021 caggaaggac acgatctcga tggggaccag caggatcagc atcggcaggg gcactttggg
    10081 cgcgaagatc gagaagaaat gcaaaccatg ccgcgccagg cccaccacga tcaccatcag
    10141 gaacacgaaa atcgcgagcg agaacgtgac gatgatgtgg ctcgtcaccg tgaagggatg
    10201 gatcagcgga aacaatccga tgacgttcga aaacagcacg aacatgaaca gtgtgaacac
    10261 gaacgggaag aatttcagtc cctcggtccc ggccgccgaa cggatcatgt cggccacgaa
    10321 cacgtagctc atttcggtga tcgattgcat gcgtgtcggc acgagacggc gcgaagacgc
    10381 gcccgcggtc agaaagagga tcgccgcggc aacgacaatg cacatccaca gcgatatgtt
    10441 ggtgaaggaa atgtcgagat tgccgacgtg caaaggcacg atcggtctca cctcgaattg
    10501 ctccatcggg ctggccacga atacccctta ttgcctcatc cgccggagcg gtcttggcgt
    10561 tgattcatct cacgcgcggt gcgcatcaaa ttgcgcatac cggccgcgac gccgagcaga
    10621 aacatcacaa tcagcagaaa tggtgaggtc ccgaaaacgc gatcgagcgc ccaaccgatt
    10681 ccgcccccca caatcactcc ggcaagaagg tccgcgacga gccggaatgc aatccccaat
    10741 tgcgattggg acccttcctg cggccgtggt gtttctttgc cacgcgcttt ttggaggcgt
    10801 tcttgcaact cggccaagcg ccttgcatcg ggatcgtctt cggccatgcg agtccaacac
    10861 cgagtcagcg ccttttttga acaacaaatt gcaggaaacg atcaaaaagg cgcgcggacc
    10921 ataggggcgg gccggatttg tgtcaaggat gtcagaattg gcgtaatgta ttgattataa
    10981 acgatgaatt atgagatgtg gcagcgtgac gcttcgcaat tgccaaggcg ggaaccgcgc
    11041 ctcgaatcag ggcgtcacga taagccgatc catcggcgtc agttgcgcca ccgccgcgat
    11101 gaggtcatcg cgcaaaaacg cgacacaacc ctgcgtcggc tcgtaatcgg ggcgcgcgac
    11161 atgcaggaaa atcgcactgc ccctgccggg cagcaccggc gaatcgttaa agcccaccac
    11221 cacgatcagg tcgtagaggg aatcttcccg ccacaggcgc tcatcgccgc tgggataggg
    11281 cagcttgacc agccggttat aatttggatc gtccggcaca tcgcaccagg cgtcatccgg
    11341 gctgattatg gcgaccgcaa gccccgtagc cggcgcctcc atgcggtccg gccggtagag
    11401 cacgcgccgg agcggccagc atcccatcgg tgtcagcccg tcgccttcgc gcaatttttc
    11461 tccgatgccg ctttgtccga ccgcgcagcg ccgctgccct atgccccact cggcgacata
    11521 aagggcaccc tcgcgtcgga caacgatatc catggccgca acgctagacg atgtcagtag
    11581 cgaaacgaag accttgccgc aggcaaggca tcttgcaagg agatggcgct ctcatacgct
    11641 tcgttcgcgc gcgccacgat tgccggatcg ctcgtccgag cgggaccatc cgatgacaag
    11701 gcggaattgc ccgcctgggc gaactgtgtt tttccagcga cgcctttttc aaggccgagc
    11761 caggaaagaa cggtggcgcc gaaatccttt ccggtggcct gcgtgaatac cacgtcggca
    11821 agcgaagcga cggcgcctgc gatgccgccg aacagggcgc cgcccgcgac ctctgccgta
    11881 ggcgacatga catcgccggt gatcgcgcga tagatcgtcc caatgatcgg gatgtgctgg
    11941 aggggattga tcgtatcgag cagatcgcca aacgtaagac cctgcgtggg ctttgcggcg
    12001 ttcgcgctcg cgaccgggct ggacgagatg ccggatgtgg ccgttcggat gtcggataga
    12061 gggggcacaa tcgttccttc gctcttaccc ggtgttaagc aagactgcgg ccagtttgcg
    12121 cccacggaaa cgggcacggt ttgtccggcc tctttcgccc ctgtcttgcg aagcaggcaa
    12181 atgttgccgc gcttgaaccc gcgctttgcc gttcgccgcc gctagaagat gtggccgctt
    12241 ttttccgctt tgacgcgcag atagtcgcgg ttgtgcgcgt tctccgggaa ggcgtgcggc
    12301 acgcgcatgg tgacttcgat gcccgcggct tcaagcgccg ccaccttgcc gggattgttg
    12361 gtgagtagcc gcaccttgct gtaacccagg agccgcaaca tgcacgcggc gatgtcgtag
    12421 agacgttcat cggcttcgaa accgaggcgc tgattggcct cgatggtgtc gaacccttcg
    12481 tcctgcagac gatacgcgcg cagcttgttc atcaacccga tgccgcgccc ctcttgcgcg
    12541 agataaagca ggatgccgcc gccttctttt gcgatcctct gaatggcgcc gcgcaattga
    12601 tcgccgcaat cgcatttcag cgacgcgaga aaatcgccgg tgaagcattc cgaatgaagc
    12661 cgcgtcagga cggggcccgg tgaggagagg gcaggggccc ccttggcgcc gatgatcacc
    12721 gcgtaatgtt ccggtccgcc gttctcggga cgaaacgcgg ccagttcggc ttgttccgcg
    12781 cccgcgagcg gaaccgaggc gcggacaacc agttgaagcg tctcggccgc gcggcgttcg
    12841 aacccatgaa tgtcggcgac cgacacttcg ctgagtgcac cggcgatctg aaggggggtg
    12901 cgcgcattgt cgggaatggg gaatagcacc gcggcaggaa ggaggccggc gagcttggcg
    12961 aggcggacgg ccgcacccgc cgattgcgga ggttgcccac gcgaggcctc gaatggacct
    13021 ttgagcggat gatcgaggtc ggtcgccgga tcggcgatgg cgcggatgtc cgctgcattc
    13081 agcctggacg gcaacgcgat gcttacaatg tccgcggtat agagccggat tttcagcgtc
    13141 gtcgccctgg catgggtcag aatcaacgcc gatggcccgc tgacgccccc aagcgcgacc
    13201 aaggtttcgc tcttgacggt ctcggccgcg acggcaatgc cgctttcccc ggccgcatcg
    13261 gcgcgaatca gcaccggcgc gccacggcga aggtcgccgg ccgcccgctc cacccgttgc
    13321 tcggggggga tcagggcctg agagatcacc ggcttaattt cgcccggttt gtctgcggtc
    13381 atgacccccg acatacaccg gtcccgggaa acgggataga gtttggcgca ttaccgtaac
    13441 gtcgcattgc aggagagttt tcgcatgccg agcacgaagc gtgttcttct cgtggacgac
    13501 gatgaaatgc tccgcgcctc attggccgaa cagcttgagg ccgatggaac ctatgagccg
    13561 gtcgaggcgg gaacctgtcg cgagggtcag cagaagggcc ttgaagggct ctatgaattc
    13621 atggtgctgg acgtctcgct tcccgatggc gatgggcgcg agctatgccg ggcttttcgc
    13681 gattccggcg tcacctgtcc gatcattatc ctgacggcgt cggattcgga tgccgacacg
    13741 atccatgggc ttcaggccgg cgccaacgac tacatcacca agccgtttcg cttctccgtt
    13801 ctcatggcgc gcgtggatgc gcatctgcgc agccacggcg cgagcgaaga ggcgatctac
    13861 aagatcggac cttatacgtt tcggcccagt gcaaaggtcc ttctggaagg cggcaagcgc
    13921 atccgtctga ccgagaagga aaccaacatc ctgaaatatc tgcaacgtgc cggttcgacc
    13981 gtcagccgcg atgtgctgct gcacgaggtg tggggttata atccggccgt ctcgacgcac
    14041 accctggaga c
//
LOCUS       DASYEG010000152        15962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2078935, whole genome shotgun sequence.
ACCESSION   DASYEG010000152 DASYEG010000000
VERSION     DASYEG010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 15962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15962
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2078935"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VGM72_06935"
     CDS             <1..538
                     /locus_tag="VGM72_06935"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4265036.1"
                     /translation="TPKNLVDLGFTVRQGIFGSVLLNVGGILGGLAFGYFAGKSHARK
                     LAPYLFVVLFVSILGYGALHSGLVPLMMGAFIAGFFMIGCMASLYAIVPRIYPARVRN
                     TGTGLAIGIGRLGAVIGPYLGGLLIAAGWQRFAYYGVLALPVLISAFAVRCIPLLGEP
                     AGQTRSSPMGNRLRPAKG"
     gene            627..1916
                     /locus_tag="VGM72_06940"
     CDS             627..1916
                     /locus_tag="VGM72_06940"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265037.1"
                     /translation="MRKFHMALAAALVGCIAGVELSRQTSAIEATPRPGAGFAAIPGV
                     VGGQDMFGPYDIVKGWPKDISTVPGNEKWTWGAGQSIYAESPDRIYVLQRGELPKIKR
                     PATKKLDEFGPSILFPIGRLPYRDATVSSPPAAGGTGQDPRDGMRLYYGKLGVDANWE
                     NCIVVINSKGDIIERWTQWDKILKRPHFVAVNPYDPEKHVWVVDDHMHAIYKFTHDGK
                     QLLQTIGTPGQEGADATHFNRPTYLAWLPDSTMFVADGYNGTRVVKFDKDGKFLMAWG
                     QKGTVPNEKRPGYMNNVHGIAVDPQTRRVFVNDRGNHRVQVFDENGKFLYDWSFGEDP
                     SDIHLIYIGADRTLWAYDRGTSKMLKYDLEGHFLYSWGTWGDFAGGFWGVHGFSVDQE
                     GNFYVAEVDSGRVQKFAPRQGVNPNFMVSKQIYSAWR"
     gene            1970..2674
                     /locus_tag="VGM72_06945"
     CDS             1970..2674
                     /locus_tag="VGM72_06945"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY4265038.1"
                     /translation="MAGQAYAAEIKVIGSPGFREAYNVLVPQFEKATGNRVTTIWDGV
                     NDVAMRVAAGETADIVILPAAQIEELTQKGKLATGSRVDVAKSGIGVAIKPGAPKPDL
                     RSGETLKAALLKAKSIAYSTGPSGVHIARLLQQWGIADVLKSRIVIAPSDTPVGEMLA
                     RGQAEIGFQQVSELIRIKGIDYLGPLPNDVQEVTVFAAALHKNAAASDAAKALVKFLS
                     APAAAETIRKAGMEPG"
     gene            complement(2688..4217)
                     /locus_tag="VGM72_06950"
     CDS             complement(2688..4217)
                     /locus_tag="VGM72_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4265039.1"
                     /translation="MKTGTAGRIDDPYGAGLKLAQQGRHAEAIALFERALARRPEDAR
                     VLFALGNTAEAVGHPDAAENFFRRVLAQDPDRLEALVHLGNLLRAKSRTADVIALLKP
                     ALERNPAEPALWLTLGSALNEAGNGTTAETFYREALRLRPGYAPALGNLADLLSDRGA
                     NGEALAMYDAVIQSDPENAQARLNRAILFLVAGELKNGWKDYESRLLLKHKFPSRDHR
                     LPRWDGVTANTRLLITAEQGIGDQIAFVSLIPELAARFARHGGRVILETEPRLVPLFA
                     RSFPDVRVHASDMETIGGRKLAHYHWLDHEGSVDCAIEMGSLPRLMRATLGDFPEPHA
                     YLAPSEERETWRRWLATQGVAPYFGLCWRSGLAGGGRNIQYAPLEAWGAFLRGVQGTL
                     VSLQYDGRADEIAKLEQISGRTILVPPHLDQKQEIDHAASLMAALDAVASAPTAVSWI
                     AAGLGVPTLKLLYKNSWTALGADYEPFAPFCRCIMPDASGDWTTTFARAANALNAIRA
                     S"
     gene            4352..4906
                     /locus_tag="VGM72_06955"
     CDS             4352..4906
                     /locus_tag="VGM72_06955"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEY4265040.1"
                     /translation="MPAKDERQASFNSGADAASPGLNSILERNIEALVERRKKEEAEC
                     TGEERIARVISRFIGSMEFVYVHVAIFGIWVAENLWDLPGIPHFDPNLVGIAITASVE
                     AIFLSTFVLINQNRMSVAQAKRADLHVQMSLLTEHELTRLLALVSAVAKQLKVRTDVD
                     RELDELKQDVAPDAVLDKLESADL"
     gene            complement(5011..5226)
                     /locus_tag="VGM72_06960"
     CDS             complement(5011..5226)
                     /locus_tag="VGM72_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265041.1"
                     /translation="MKMIAMTLSAVAVAMTLSACAYDRYDRSGYYGNDRYDRHNGYRY
                     NDRGYNYDNDRGHDFDRDRYDRDRDRD"
     gene            complement(5321..5710)
                     /locus_tag="VGM72_06965"
     CDS             complement(5321..5710)
                     /locus_tag="VGM72_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4265042.1"
                     /translation="MIVNEKTHVSSAILVVEDEPLLLMIVSETLRDAGYAVWEAGDAE
                     SALGIIKAEPDIDLLISDVKMPGLSGYQLVDASLSLRPDLKIMLMTGYAQDPIPKKIV
                     QAGIQVLYKPFDFDKLPDFAHEILERE"
     gene            complement(5710..7080)
                     /locus_tag="VGM72_06970"
     CDS             complement(5710..7080)
                     /locus_tag="VGM72_06970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4265043.1"
                     /translation="MLAMTKNLLRKKLNPSLALLSALALVLAGVLVTAYEEQLYRAQK
                     VQEVNAQGQILAASVAAALVFDDEKAAQEYVAALRTNPELEAAGVYGGNGAPVAGFAR
                     DGSEPLPRRAGAGTSYVSNNRVYTIIVVNQGGARVGAVYLRANLDSIGRRLVRYGGII
                     LLVTMGVLVLAVSGGAQRALAAANTRLESQARQLAASNQKLQAEMQEREKAEDALRQS
                     QKMEAIGQLSGGIAHDFNNLLSIIKGNLQLLQRRLSQGRTDTQRYLDAANEGVNRAAG
                     LTQRILAFSRRQPLSPKPVQLSRLVENMGELLRHSIGDAVRIEMRLTADWAVLCDANQ
                     MENVIINLAINARDAMPSGGTLTIATANRSTGERPGLPLRDYVELMVRDTGTGMPDDV
                     LQKAVEPFFTTKPPGRGTGLGLSMTFGYVRQSNGEMIIESELGKGTTIMILMPRYEME
                     TVPVSV"
     gene            complement(7095..7571)
                     /locus_tag="VGM72_06975"
     CDS             complement(7095..7571)
                     /locus_tag="VGM72_06975"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HEY4265044.1"
                     /translation="MAAPAETLEYAVKATFVYKFAGFVDWPATSFESPSSPVTICVLG
                     SDPVAALIDQAAAGRQVGDRTVAVKYLQIFVRDSGCNILYVAANMPGDAANAARGAPV
                     LTVGASPRAEAGGTIVTFVVQDNRVRFDIDDAAAAENGLAISSKLLSLARAVRPRP"
     gene            complement(7651..9549)
                     /locus_tag="VGM72_06980"
     CDS             complement(7651..9549)
                     /locus_tag="VGM72_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4265045.1"
                     /translation="MFRNTIVCACLLSCTAPAAAQQVAAVEDLGQLSIDELANLQITA
                     VGRQPQPLSQAPAAVYVITNEDIRRSGAASVAEALRLAPNLQVARLNSSTYAISARGF
                     NHNTATANKLQVMIDGRVVYTPLYAGVFWDQQDVPLADIDRIEVISGPGGAVWGSNAV
                     DGVINIVTRNSRDTQGALVDLQGGTLDQLATVRYGGKLGEDATFRIYTMGAKRSALES
                     PIGTATGDSWDKIQGGFRSDWSHARDAVTFQGDIYNATTQDQPGMLQNGAVSGGNVVG
                     RWNRQFDAGSMLQTQLYYSRSERNTTSGIRSTVNNYDLDLQYSFAAGSGQNFVVGAGE
                     RMTQDVFTAGPGTAFLDPAKRTLNLANVFLQDQIDLAPDLKLVLGLKAEHNDYTGMEY
                     MPDARLSWRLLGTDLLWGAVSRAARTPSRFDHDLFAMNTLAGGPNFISENLTAYEIGY
                     RAQPSNAMSLSISAFYNVYDHLRTVEASTPAIFPLVVLNNMKGATYGIELWANYGVAR
                     WWRLSAGLSTLHKDLRLEQGSRDIFGVQFAGNDPSYQASLRSSMDVADNIELDIYLRA
                     VDDLPSPRVPGYAEADVRLGWRVTNSLELALEGSNLLHARHTEFIDPSLPVEEIPRSI
                     TASARWRF"
     gene            complement(9674..9750)
                     /locus_tag="VGM72_06985"
     tRNA            complement(9674..9750)
                     /locus_tag="VGM72_06985"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9714..9716),aa:Arg,seq:cct)
     gene            9947..11533
                     /locus_tag="VGM72_06990"
     CDS             9947..11533
                     /locus_tag="VGM72_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY4265046.1"
                     /translation="MTVMAVLARAKMVIMRHRVTIQDGSLLVAVLLLMTYLAFEIDVF
                     ENAPNVPPQEQVIELDEGLGLGALFAAGMLIFAGRRYQEQKREMARRIAAEREARTLA
                     LHDALTGLPNRRQFDSALKAAIAAMPRAGACHAVFLLDLNGFKKVNDVHGHGAGDEVL
                     VQVAGRLSKAMREGDLIARFGGDEFAVLAPQLSSAEDATTIALRIIDALKEPVATGLA
                     QHRIGTGIGIALIPQDSDAAEDILRKADIALYRAKTAGMSALRFFEEEMDARVQERDF
                     LENELRAAIGTAALRPYYQPIEDLNTKRIVGFEALARWTHATAGEISPERFIPIAEDC
                     GLIGELSDYLFRQACRDAIAWPGDITLSFNISPAQLRDRTLGLRVLAILGETGLSPRR
                     LELEITESALVRDMEAAQETLGALRQSGIRIALDDFGTGYSSLYHLRNFKLDKIKIDR
                     SFIEKMSTDRESETIVRALVGLGTGLSLTVTAEGVENKGQQMQLIAEGCQQAQGFLFS
                     EALPQAGAMELLKGRQRRRA"
     gene            11630..11920
                     /locus_tag="VGM72_06995"
     CDS             11630..11920
                     /locus_tag="VGM72_06995"
                     /inference="COORDINATES: protein motif:HMM:NF019370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HEY4265047.1"
                     /translation="MDEQTNLVDLSMAVPEDAAALMPLLPDIQPEFETQVPMARKKKF
                     ITTMTLTSETCKWPFGDPVSPDFHYCGHAPQAGRPYCSTHEAISRPSSLRRK"
     gene            12295..13290
                     /gene="hpnA"
                     /locus_tag="VGM72_07000"
     CDS             12295..13290
                     /gene="hpnA"
                     /locus_tag="VGM72_07000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HEY4265048.1"
                     /translation="MTTDRVLISGASGFVGSAVARAAAARGFRVRALVRAQSPRGNLA
                     GLDCEIAEGDMKDAASMRRALKDVRYFFHVAADYRLWARDSTEVYRNNLEGTRIAMEA
                     ARACAVERIVYTSSVATLKPIAGGVADETARLEESQAIGGYKRGKVAAHRLVEAMVTD
                     HGLPAVIVSPSTPIGPRDIRPTPTGRIIVEAASGRMPAFVETGLNLVHVEDVAEGHML
                     ALEKGAKGESYILGGQDATLKEMLTIIAALSGRRAPRVKLPRGPLFPMAYAAEAVARL
                     TGKEPFITADGLKMAKYRMFFSSAKAATTLGYRARPYAQGLEEAFVWFRQAGYLR"
     gene            13287..14429
                     /locus_tag="VGM72_07005"
     CDS             13287..14429
                     /locus_tag="VGM72_07005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4265049.1"
                     /translation="MTPATLVSFGALAAWIYLLACRGMFWHMRERDDGEVIYSGAGWP
                     SVVAIVPARDEAATIARVLGSLMAQDYPGNFRIVLVDDQSSDETAERARALDANGRLE
                     VLRGAEPPSDWTGKLWALKQGVVQAAKAAPDYFWFTDADIAHAPDNLRQLVARAQQSG
                     LVMVSLMAKLRCESFAERLLIPAYVFFFAMLYPFSWVNRAGNSTAAAAGGCILLRRDA
                     LERAGGIDAIRGEIIDDCALARRMKRQGPIWLGLSARALSLRPYPRLRDIGQMVARSA
                     YAQLGYSPLLLLGTLAGMIAIYAAPPFIAVLGTGPARLAAGASWLFMAAAFQPMLRFY
                     RRSPVWGLTLPLIGILYTAFTIDSAIQYWRGRGGMWKGRFQTMKRA"
     gene            14426..15310
                     /gene="hpnC"
                     /locus_tag="VGM72_07010"
     CDS             14426..15310
                     /gene="hpnC"
                     /locus_tag="VGM72_07010"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEY4265050.1"
                     /translation="MIEQAAELGSGKGHRDENFPVASVLIAPRHRPIILAFYRFARVA
                     DDVADNAAAPADEKLRLLDGMRRTLLGELDTQPEASVLRVMLAARGLSPQHGLDLIEA
                     FSRDVTKLRYRDWNDLMDYCHVSAMPVGRFVLDVHGEPRTLWQACDALCAALQIINHL
                     QDCAKDYRELNRVYIPLDGFAAAGLGPESLNEPRASASLRGLIAGLAKRTSELLVQSR
                     NFAAGVRDSRLAFEISVIQVLAEDLCRRLIQRDPLCERVHHRKSEIAALAALAAWRFG
                     AGRLRRRQVDIVIADERR"
     gene            15297..>15962
                     /locus_tag="VGM72_07015"
     CDS             15297..>15962
                     /locus_tag="VGM72_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEY4265051.1"
                     /translation="MSGASGVQAGGPQSGFDPRAAQTRASGSSFYAGMRLLPKAERDA
                     MFAIYAFSRAVDDIADEGHDSRASRHAALDDWRRDIEQLYAGSPTKRVEFLVPVVRVY
                     GLRKEDFLAIIDGMDMDVAEDIRAPDLAMLDLYCDRVASAVGRLSVKIFGMDDAPGFK
                     LAHHLGRALQITNILRDLDEDAAAGRLYLPREFLAEAGIGARDPSAVIANTWIDRACR
                     RLAA"
BASE COUNT         2980 a         4823 c         5039 g         3120 t
ORIGIN      
        1 gacacccaag aacctggtcg atcttggctt cacggtgcgg caagggatct tcggctccgt
       61 gctgctcaat gtcggcggca tcctgggtgg gcttgccttc gggtatttcg cgggtaagtc
      121 ccatgcgcgc aagctcgcgc cctatttgtt cgtcgtgctc ttcgtctcga ttctgggtta
      181 tggcgcgttg cactcgggcc tcgtgccgct gatgatgggc gcctttatcg ccgggttttt
      241 catgatcggt tgcatggcga gcctctatgc catcgtgccc cggatttatc cggcgcgcgt
      301 ccgcaatacg ggaaccggac tggccatagg catcgggcgg cttggcgcgg tgatcggccc
      361 ctatctcggc gggctcttga tcgcggccgg gtggcagcgt tttgcttatt acggcgtgct
      421 cgcgctgccg gttttgattt cggcgttcgc ggtcagatgc attcccctcc ttggcgagcc
      481 cgccggacaa acgcgttcga gcccgatggg caatcgcttg cggcccgcca agggttagga
      541 gcgtttcctg ccccgcgtcg ccgttcgcgt gcgtttgtgg taatccttgc ccggaaaaga
      601 aaaaatcacg ggggcggagg ggacacatgc gcaaattcca tatggcattg gcggcggcgt
      661 tggttgggtg catcgcgggc gtcgaattgt ctcgccagac gtcggccatc gaggcgacgc
      721 ccaggcccgg cgccggattc gccgcgatcc ccggcgtggt cggcgggcag gacatgttcg
      781 gcccctatga catcgtgaaa ggctggccca aggacatcag cacggtgccg ggcaacgaga
      841 aatggacctg gggcgcgggc cagagcatct atgcggaaag tccggatcgc atctatgtgc
      901 ttcagcgcgg cgaattgccg aagatcaagc ggcccgcgac caagaagctc gacgaattcg
      961 ggccgagcat cctgttcccc atcggccggc tgccttatcg cgatgcgacc gtgtcctcgc
     1021 cgccggccgc cggcggcacc ggacaggatc cgcgcgacgg catgcggctc tattacggca
     1081 agctcggcgt cgatgcgaat tgggaaaact gcatcgtcgt gatcaattcc aagggcgaca
     1141 tcatcgagcg ctggacgcaa tgggataaaa tcctgaagcg tccgcatttt gtcgccgtca
     1201 acccttacga cccggaaaag catgtctggg tggtcgatga ccacatgcac gccatctaca
     1261 aattcaccca tgacggcaag cagttgttgc agaccatcgg cacgccggga caggaaggcg
     1321 cggacgcgac acatttcaac cggccgacct atctcgcctg gttgccggac agcaccatgt
     1381 tcgtggccga cggctacaac ggcacgcggg tcgtcaaatt cgacaaggac ggcaagttcc
     1441 tcatggcctg gggccagaag ggaacggtcc cgaatgaaaa gcgccccggt tatatgaaca
     1501 atgtgcacgg catcgcggtg gacccgcaga cgcggcgcgt cttcgtcaac gatcgcggca
     1561 atcatcgcgt gcaggtgttc gatgagaacg gcaagttcct ctacgattgg agcttcgggg
     1621 aagatccgtc cgatatccat ctgatctaca tcggtgcgga ccgcacgctc tgggcctatg
     1681 atcgcggcac ctccaagatg ctgaaatacg atctggaagg gcacttcctc tattcctggg
     1741 gcacatgggg agattttgcc ggcggcttct ggggcgtgca cggattttcc gtggatcagg
     1801 agggcaattt ctacgtcgcc gaagtcgatt ccggacgcgt gcagaaattc gcgccccggc
     1861 agggcgtgaa tccgaacttc atggtcagca aacagatcta ttcggcctgg cgatgagggc
     1921 aagaaaacct ttccccttgt gggcacaggc tttggctctg agtgtcgtta tggctggcca
     1981 agcctatgcc gccgagatca aggtgattgg gtcgccgggt tttcgcgaag cctataatgt
     2041 gctggtgccg caattcgaga aagcgacggg gaatcgcgtc acgacgatct gggacggggt
     2101 gaacgatgtc gcaatgcgcg tggcggcggg cgaaaccgcc gatatcgtga tcctgccggc
     2161 ggcgcaaatc gaggagctga cccaaaaggg caagctcgcg acggggagcc gtgtcgatgt
     2221 cgcgaaatcc ggcatcggcg tggccatcaa acccggcgcg cccaaacccg atttgcgctc
     2281 cggcgaaacg ctgaaggccg ccttgctcaa ggcaaaatcg atcgcctatt cgaccgggcc
     2341 aagcggcgtg cacatcgcgc ggctgttgca gcaatgggga attgccgatg tgcttaagtc
     2401 ccggatcgtc atcgcgccga gcgacacgcc ggtgggtgag atgctcgcgc gcggtcaggc
     2461 tgagatcggt ttccagcagg tgagcgaatt gatccgcatc aaaggcatcg actatctcgg
     2521 acctttgccc aacgatgtgc aggaggtcac tgttttcgcc gcggcgctgc acaaaaatgc
     2581 tgccgcatcc gacgcggcga aagctctggt caaattcctg agcgcgccag cggcggccga
     2641 gaccatccgc aaagccggca tggagccggg ttgattctcg cgcgtgccta ggaggcgcgg
     2701 atcgcgttga gtgcgttcgc ggcgcgcgca aacgtcgtcg tccaatcgcc gctggcgtcg
     2761 ggcatgatgc agcggcaaaa cggcgcgaaa ggttcatagt ccgcgccgag tgccgtccaa
     2821 ctgtttttgt agagcagttt cagcgtgggc acgcccaagc cggccgcgat ccatgagacg
     2881 gccgttggcg cgctcgccac cgcatcgagc gcggccatga gcgaagccgc gtggtcgatc
     2941 tcctgttttt gatcgagatg cggcgggaca agaatcgttc gcccgctgat ctgttccaat
     3001 tttgcgatct catcggcgcg gccatcatat tgcagcgaca ccagcgtgcc ctgaacgccg
     3061 cgcaggaatg cgccccacgc ttcgagcggc gcatattgaa tgttgcgccc gccccccgcg
     3121 aggccgctgc gccagcacag gccgaaatag ggcgcgacac cttgcgtggc gagccagcgc
     3181 cgccaggtct cgcgttcctc ggaaggcgcc agataggcgt gaggttccgg aaaatcgccg
     3241 agcgtggcgc gcatgagccg tggcagcgag cccatctcga tggcgcaatc cacactgccc
     3301 tcatggtcga gccaatgata atgcgcgagc tttcgcccac cgatcgtttc catatccgac
     3361 gcgtggacgc gcacgtccgg aaaagagcgc gcgaacagcg gcacaagacg cggctcggtc
     3421 tccaggatca cgcgcccgcc gtgacgcgca aatcttgcgg ccagttccgg gatcaggctg
     3481 acaaacgcga tctggtcgcc gatcccttgt tcggccgtga tcagcagccg cgtgttcgcc
     3541 gtgacaccgt cccaacgggg caggcgatgg tcgcggctgg gaaatttgtg cttgagcagc
     3601 aggcgggatt cgtaatcctt ccagccgttt ttcagttcgc ccgcgaccag gaaaaggatc
     3661 gcgcgattga gccgggcctg cgcgttctcc ggatcgctct ggatgacggc atcgtacatc
     3721 gcgagggctt cgccgtttgc gccgcggtcg gaaagaagat cggcgagatt gccgagcgcg
     3781 ggcgcatagc cggggcgaag gcgcaacgcc tcgcggtaga aggtttcagc ggtcgttcca
     3841 ttgccggctt cgttgagtgc cgaacccagc gtcagccaca gcgcgggctc tgccggattg
     3901 cgttccagtg cgggcttcag cagcgcgatc acatcggctg tgcgactctt ggcgcgcaac
     3961 agattgccga gatggacgag tgcttccagc cggtcgggat cctgggccag cacgcggcga
     4021 aagaaattct cagccgcatc ggggtgaccg accgcttcgg cggtattgcc gagcgcgaac
     4081 agcacgcgcg catcttcggg gcggcgcgcg agggcacgct cgaacagcgc aatggcttcg
     4141 gcgtggcggc cttgctgggc gagcttcagc cccgccccat agggatcgtc gatacggccg
     4201 gcggtgcccg ttttcatggg cgaaagctaa tcggcggcgc gttaagaccg gattaaccgc
     4261 gtttggccag gactggcgcc aaactggagc gtagcggaac ccgcaaggca cacgctgcgt
     4321 accctattgc cgcgcgcagc gagggttttt cgtgcctgca aaagacgaac gccaagcctc
     4381 atttaattcc ggcgcggatg ccgcgtcccc gggactcaat tccatcttgg agcgcaatat
     4441 cgaggcgctg gtcgaacggc gcaaaaagga agaggccgaa tgcacggggg aagaacgcat
     4501 cgcccgcgtg atttcgcgct ttatcggcag tatggagttc gtttacgtgc atgtcgcgat
     4561 ttttggaatc tgggtcgccg agaatctgtg ggatctgccg ggcattccgc atttcgatcc
     4621 caatcttgtc ggcatcgcga taaccgcctc ggtggaagcg atcttccttt ccaccttcgt
     4681 gctcataaac cagaacagaa tgtccgttgc gcaggccaaa cgcgcggacc tccatgttca
     4741 gatgagtttg ctcaccgaac acgagctgac ccgtttgctg gcgctcgtct ccgcggtggc
     4801 gaagcagctt aaggtccgca ccgatgttga ccgcgaactg gacgaactca aacaggatgt
     4861 ggcgcccgat gcggttctcg acaaactgga aagcgcggat ctgtaacggc gcagatctat
     4921 aacggcacag atctgtaaca acacggggcg cgtgcaccgc acccgccccg agccccggca
     4981 taccgatacc ggcatattaa tcctggcgta ttaatcgcgg tcgcgatccc ggtcataacg
     5041 gtcgcgatcg aagtcgtgac cgcgatcatt gtcataattg tagccacgat cattgtagcg
     5101 gtacccatta tggcgatcgt accgatcgtt gccgtaatag ccggaacgat cgtaacgatc
     5161 gtaagcacaa gcggacaatg tcattgccac ggctaccgcg ctgagcgtca ttgcgatcat
     5221 cttcatgggt caaactccct atatcgtcga tacggctacg cataagggcc ggcgcttgtt
     5281 ccataagttc gcaaaggcgc ggtccttggc cggtatggaa ctattcgcgt tcgagaatct
     5341 cgtgagcgaa atccggcagc ttgtcgaaat cgaacggctt gtagagaacc tggattcccg
     5401 cttggacgat ttttttcgga atcgggtcct gcgcgtaacc cgtcatcagc atgatcttca
     5461 ggtccggccg caacgagagg ctggcatcga caagctgata tccgctcaag cccggcattt
     5521 tcacatcgct gataagcagg tcgatatcgg gttcggcttt aataatgccg agagcggatt
     5581 ccgcgtcgcc cgcttcccag acggcatatc cggcatcgcg caacgtctcg ctgacgatca
     5641 tcagcagcaa cggttcgtcc tcgaccacga gaatggcgga ggacacgtgg gttttttcgt
     5701 tcacgatcat cagacgctca ccggtacggt ttccatttcg tagcgcggca tgagaatcat
     5761 tatcgtggtt cccttgccca attcactttc aatgatcatc tcgccattgg attggcgcac
     5821 ataaccgaag gtcatactca atccaaggcc ggtgccgcgg cccggcggtt tggtggtgaa
     5881 gaacggctcc acggccttct gcaacacatc atcgggcatc cctgttccgg tgtcgcggac
     5941 catgagttcc acgtaatcgc gcaaaggcag tccgggacgc tcgcctgttg agcgattcgc
     6001 agtcgcgatg gtcaatgtcc ctccggaggg catggcgtca cgcgcgttga tggcgagatt
     6061 gatgatcaca ttttccatct gattggcatc gcacaagacc gcccaatcgg cggtcaggcg
     6121 catctcgatg cggactgcat cgccgatgga atggcgcagg agctcgccca tattctcgac
     6181 gagccgactc aactggaccg gcttgggcga aagcggctgc cgccgggaaa aagctaggat
     6241 gcgttgcgtc agacccgcgg cgcggttgac gccttcgttc gcggcatcca gatagcgctg
     6301 agtgtccgtt cgtccttggg acaggcgccg ctgcagcaat tgcagattgc ctttgatgat
     6361 gctgagcaga ttgttgaaat catgggcgat gccgcccgag agctggccga tggcttccat
     6421 cttctgactt tggcggagcg cgtcttcggc tttttcgcgc tcctgcattt cggcttgcag
     6481 cttctgattg gacgcggcca attggcgcgc ctgactttcg agccgtgtat tggcggctgc
     6541 gagcgcgcgc tgtgccccac ccgaaaccgc gagcaccagc acacccatcg tcaccagaag
     6601 aatgatgccg ccataccgaa ccagacgccg gcctatgcta tccagattag cgcgcaaata
     6661 gaccgcgccc acccgggcgc cgccctgatt gacgacgatg atcgtatata cgcggttgtt
     6721 ggagacatag gacgtgccgg ctccggcacg cctgggcagc ggttcggaac catcgcgcgc
     6781 gaaacccgcg accggcgcgc cattgccgcc atacaccccg gcggcctcca gctcgggatt
     6841 ggtccggagt gccgccacat attcctgggc cgccttctcg tcgtcgaaga ccagggccgc
     6901 cgcgacgctg gctgcgagga tctggccttg tgcgttgacc tcctggacct tctgggcacg
     6961 ataaagctgt tcttcatagg ccgtcacaag cacgcccgcc aaaaccagcg caagcgcgct
     7021 gaggagcgcg agcgagggat tgagcttctt gcgcaacaga tttttggtca tcgcgagcat
     7081 gtgagtcacg ctccctaagg ccgtggcctg accgcgcggg ccaggctcag cagtttcgaa
     7141 ctgatcgcaa gaccgttctc ggcggcggcg gcatcgtcga tgtcaaatcg cacgcgattg
     7201 tcctgcacga cgaaggtgac gatggttccc cctgcctcgg cgcgcggcga tgcgccgacg
     7261 gtcagcacag gtgcgccgcg cgcggcattg gccgcgtcgc cgggcatgtt cgccgcgaca
     7321 tagagaatgt tgcacccgga atcgcggacg aatatctgaa gatacttcac cgctacggtg
     7381 cggtcaccca cctgccggcc ggcagcggcc tgatcgatca gcgcggcgac gggatcgctt
     7441 ccgaggacac agatcgtaac gggactggaa ggggactcga acgaagtggc tggccaatcc
     7501 acgaagccgg cgaatttgta gacgaatgtc gccttcaccg catattcgag cgtctccgcc
     7561 ggagccgcca cggatgatcg gcaagccagc gccagcgtta cgatgcaaat ggcagaaaaa
     7621 attcgcccgc gcattaccgg aaagcgtccc tcaaaatcgc cagcgggcac tggccgttat
     7681 gcttcgtggt atctcctcga cggggagcga tggatcgatg aattccgtat ggcgcgcgtg
     7741 aagcagatta gaaccctcca gcgcaagttc cagggaattg gtgacacgcc agccaaggcg
     7801 cacatcggct tcggcataac ctggtacgcg cgggctcggc aggtcgtcca ccgctctcaa
     7861 atagatgtcc agttcgatat tgtcggctac atccatgctg gagcgcaagg aggcctgata
     7921 ggaaggatca ttgccggcga attgcacgcc gaaaatgtca cggctgccct gctccaggcg
     7981 cagatccttg tggagcgtgc tcaacccggc gctcaggcgc caccagcgcg ccaccccgta
     8041 attggcccat agctcgatgc cgtaggttgc gcccttcatg ttgttgagca cgacgagggg
     8101 aaatatcgcc ggcgtcgatg cttcgacggt tcgaaggtga tcgtacacat tgtagaaagc
     8161 cgaaatcgac aaggacatcg cgttcgacgg ctgcgcccga tagccgattt cataagcggt
     8221 cagattttcc gagatgaagt tggggccgcc cgcgagggtg ttcatggcga acaggtcgtg
     8281 atcgaagcgt gacggcgtgc gcgccgcacg gctgacggcc ccccacagca aatcggtgcc
     8341 cagcagccgc caggagaggc gcgcatctgg catgtactcc atgccggtat agtcattgtg
     8401 ctctgccttc aggcccaaga cgagcttcag atccggagcg aggtcgatct gatcctgcag
     8461 aaagacattg gcgaggttca gagtgcgctt cgccggatcg aggaacgcgg ttccagggcc
     8521 ggcggtaaaa acgtcctgcg tcatccgttc gcccgcgccg accacaaaat tctgccccga
     8581 cccggcagca aagctgtact gaaggtccaa atcatagttg ttgaccgtgg accggatgcc
     8641 cgaagtggtg ttgcgctctg aacgggaata atagagctgg gtttgcagca tggagcctgc
     8701 gtcgaattgg cggttccagc gcccgaccac attgccgccg gaaacggcgc cattttgcag
     8761 catgcccggc tggtcctgcg tggtggcgtt gtagatatcg ccctgaaaag tcacggcatc
     8821 ccgggcgtgg ctccagtcgg agcggaagcc tccctggatc ttgtcccagg aatctccggt
     8881 tgcggtgccg ataggcgatt cgagggcgct gcgtttggct cccatcgtat agatgcgaaa
     8941 ggtcgcgtcc tcgcccagtt tgccgccgta acgcacggtg gcgagctgat cgagcgtacc
     9001 cccctgcaga tcgacgaggg cgccctgcgt gtcccgggaa ttgcgggtga cgatgttgat
     9061 gacgccgtcc acggcatttg agccccaaac ggcgccgccc ggcccgctga tcacctcgat
     9121 gcggtcgatg tcggccagcg gcacgtcctg ctgatcccag aagacgccgg catagagggg
     9181 cgtgtaaacg acgcgtccat cgatcatcac ctgcagcttg ttggcggtcg cggtgttatg
     9241 gttgaaaccg cgcgcgctga tcgcataggt cgaggaattg aggcgggcaa cctgcaaatt
     9301 gggcgccaaa cgcaacgctt cggcaacgct ggcggcaccg gagcggcgga tgtcttcgtt
     9361 ggtgatgacg tagaccgctg cgggggcctg gctgagcggc tggggttggc ggccgaccgc
     9421 ggtgatttga agattggcca gctcgtcgat cgacaattgg cctagatctt cgacagcggc
     9481 aacctgttgt gcggcggcag gcgccgtgca actcagcaga cacgcacata cgattgtatt
     9541 gcgaaacacg cttagccccc gccgcgcagt ccaccccaag cgcggaacgt gagtatacgc
     9601 ttacgctcgc atcgaagttc cctggatttt tcaacaaaaa ccagggcact ggccagggtt
     9661 ggcgcgaaag tcgtggcgac cccgattgga ttcgaaccaa cggcctacag attaggaatc
     9721 tgtcgctcta tcctgctgag ctacggggtc ttagggggcc ggtcagccac cccaatgtct
     9781 tgctactgta tcggcctcgg caaacgaatt gtagccagaa aatccatgtc aaaacctggc
     9841 aaccctcacg ccggtgcacg gtgctctcca ccgtgtcctt gccttaagcc cttcttaaac
     9901 gctgcacttt atctctcggc atggtttttt ttcggagaac ccgcgcatga ccgtcatggc
     9961 ggtgctggcg cgcgccaaga tggtgatcat gcgtcatcgc gttacgattc aggacgggtc
    10021 gctgcttgtg gccgtcctgc tcttgatgac ctatctcgcc ttcgaaatcg atgttttcga
    10081 gaacgctccg aacgtcccgc ctcaggagca ggtgatcgag ctggatgaag gattggggct
    10141 cggcgcgcta ttcgccgccg gcatgctgat cttcgcaggc cgccgctatc aggagcagaa
    10201 gcgcgaaatg gcgcgccgca tcgcggccga gcgcgaggcg cgtacgcttg cgctgcatga
    10261 cgcgctgacc gggttgccga atcggcgcca gttcgacagc gcgctaaagg cggccattgc
    10321 ggcgatgccg cgggcaggag cctgccatgc ggtcttcctg ctcgatctga acggcttcaa
    10381 aaaggtcaat gacgtgcacg gtcatggcgc cggcgatgaa gtcctggttc aagtcgcggg
    10441 gcgattgtcg aaggccatgc gtgagggcga tttgattgcc cgcttcgggg gcgatgaatt
    10501 tgccgtcctg gcgccgcagc tttccagcgc ggaggacgca accaccattg cgctgcgaat
    10561 catcgacgcg ctcaaggaac ctgtcgccac cggtctggcg cagcacagga tcggaaccgg
    10621 catcggcatc gcgctgatcc cccaggatag cgatgcggcc gaggacatct tgcgcaaggc
    10681 cgatatcgcg ctgtatcgcg ccaagacggc gggtatgtcg gcccttcgct ttttcgaaga
    10741 ggaaatggat gcccgcgtgc aggaacgcga tttcctcgaa aacgaactcc gcgcagccat
    10801 cggcaccgct gcgttgcggc cctattatca gccgatcgag gacctgaaca ccaagagaat
    10861 cgtcgggttc gaggcgctgg cgcgctggac gcatgcgacc gccggcgaga tttcgccgga
    10921 gcgtttcatc ccgatcgccg aagactgcgg cctgatcggc gaactcagcg attatctttt
    10981 tcgccaagcc tgccgcgatg cgattgcgtg gcccggcgac atcacgcttt cgttcaatat
    11041 ttcaccggct cagcttcgag accgcacgct cggtcttcgg gtattggcga ttttgggcga
    11101 gaccgggctt tcgccgcgcc ggctggaatt ggagatcacc gagagcgcgc ttgtacgcga
    11161 catggaggcg gcgcaggaaa cgctcggcgc gcttcgccaa tcgggcatcc gcatcgcgct
    11221 ggacgatttc gggaccggat attccagcct ctatcatttg cggaatttca aactcgacaa
    11281 gatcaagatc gaccgtagct ttatcgagaa aatgtcgacc gaccgcgaga gcgagacgat
    11341 cgtgcgcgcg ctggtcggat tgggaaccgg cctctcactc acggtgaccg ccgagggcgt
    11401 ggagaataag gggcagcaga tgcagctcat cgcggaaggc tgccagcagg cgcaagggtt
    11461 tctcttcagc gaagccctgc cgcaagcggg cgcgatggaa cttctgaagg gccgccagcg
    11521 ccggcgtgcc taatcaaacc ttgccgcgcg aagcctgctc tttacgttgc ataaaccttg
    11581 ttggaccaac atcggggctc attgcactct gacgcgtatg gagagcccga tggacgagca
    11641 gacgaacctt gtagacctgt caatggccgt tcccgaagac gccgcagcgt tgatgcctct
    11701 tttgccggac atccagccgg aattcgagac acaggttccg atggcgcgta agaaaaaatt
    11761 catcaccacg atgaccctga ccagtgaaac ctgcaaatgg ccgttcggcg atccggtctc
    11821 gccggacttc cattattgtg gacatgcgcc gcaagccggg cgtccctatt gcagcaccca
    11881 cgaagccatc agccggccaa gttcgctccg ccgcaaatag gccgacaagc ttcccatcat
    11941 cggtgctgaa aggtgaactg gacgaggggg ccggaccgaa tgccacgccg atcgggacat
    12001 cggcggcatc gcgccattgc gcttgggcaa attgcctctt acgttgtcgc ttgcaacgta
    12061 acgaaaggag gtgatccagt gtctcattgt cttcatctgc ggtcagatgg ctgggtcgtc
    12121 gcctctgtga ggaactcctt cgcatagcga cgtgtcttgc ggtttcgcac cgcagttaag
    12181 caatggaagg gctctcccgc gggagggccc ttctttttgg catttgcggc caagaaaaga
    12241 tcgccttgcg gatgaaagcg ggtatgggat tatccgcctc tccaggaaga ggccatgaca
    12301 acagaccgcg tattgataag tggtgcttcc ggcttcgtcg gctccgcggt cgcccgggca
    12361 gccgcggcgc gtggttttcg cgttcgcgcc ctggtgcgcg cccagagccc gcggggtaat
    12421 ctcgccggtc tcgattgcga gatcgccgaa ggcgacatga aggacgcagc ttcgatgcgc
    12481 cgcgccttga aggatgtccg ctatttcttt cacgtcgccg ccgattaccg gctgtgggcg
    12541 cgcgattcga cagaagttta ccgcaacaat ctcgaaggga cccgcatcgc gatggaggcg
    12601 gcgcgtgcct gcgccgtcga acggatcgtt tacaccagca gcgtcgcgac cttgaagccg
    12661 attgcgggcg gcgtcgccga cgaaaccgcg cgcctggaag aaagccaggc catcggcggc
    12721 tacaaacgcg gcaaagtggc agctcaccgg cttgtcgaag ccatggtgac ggaccacggt
    12781 cttcccgccg tgatcgtcag cccgtccacg ccgatcggac cgcgcgacat tcggcccacg
    12841 cccacgggac gcatcatcgt cgaggcggcg tcggggcgga tgccggcttt tgtggaaacc
    12901 ggcttgaacc tcgtccatgt cgaggatgtg gcggagggcc atatgctcgc gcttgagaaa
    12961 ggcgcgaagg gcgagagcta tatattgggc ggccaggatg cgacgctgaa ggagatgctg
    13021 acgatcatcg cagcgctgtc gggccggcgc gcaccgcgcg tgaaattgcc gcgcggcccc
    13081 ctgttcccga tggcctatgc ggctgaagcg gtcgcacggc tgacggggaa ggaaccgttc
    13141 attaccgccg acgggcttaa aatggccaag taccgcatgt ttttttcctc cgccaaggcg
    13201 gcaaccacgc ttggctatcg cgcgcggccc tatgcgcagg gccttgaaga ggcgttcgtc
    13261 tggttccggc aggcgggcta tttgcgatga cgccggcaac gcttgtcagc tttggggcgc
    13321 tggccgcctg gatttatctg ctcgcatgtc gtgggatgtt ctggcacatg cgcgaacgcg
    13381 atgacggcga ggtcatatat tccggggccg ggtggccttc ggttgttgcc attgtgccgg
    13441 cgcgtgacga agccgccacc atcgcgcgag tcctgggcag cctgatggcg caggattatc
    13501 ccggcaattt tcgtatcgtg ctggtggacg atcagagcag cgacgagacg gcggagcgcg
    13561 cccgggcact cgatgccaat gggcggctgg aggtcttgcg tggcgctgag ccgccgtcgg
    13621 attggaccgg gaaactctgg gcgctgaagc agggcgtcgt gcaggcggca aaagccgcgc
    13681 ccgattattt ctggttcacc gatgccgaca tcgcgcacgc cccggacaat ctgcgccagc
    13741 ttgtcgcgcg cgcccaacaa agcggactcg tcatggtctc gctgatggcg aaattgcgct
    13801 gcgagagttt cgccgaacgc ttgctgatcc cggcctatgt gtttttcttc gccatgctgt
    13861 atccgttttc ctgggtcaat cgcgcgggca attccaccgc cgcagctgcc ggcggctgca
    13921 ttctgctccg ccgtgacgcg ctggaacgcg ccggggggat cgacgcgatc cgaggcgaaa
    13981 tcattgacga ttgcgcgctg gcccggcgca tgaagcgcca aggaccgatc tggcttggct
    14041 tgagcgcgcg cgcgttgagc ctgcgcccct atccgcgatt gcgcgatatc ggacagatgg
    14101 tcgctcgttc ggcctatgcc cagctcggtt attcgccgct tctgcttctg gggacgctgg
    14161 ccggcatgat cgcgatctat gccgcgccgc ccttcatcgc cgtcctgggc acggggcctg
    14221 cgcgtcttgc cgcgggcgca tcctggcttt tcatggctgc cgcctttcag cccatgctgc
    14281 gtttttatcg ccggtcgccc gtttggggat tgacgcttcc cctcatcggt attctctaca
    14341 ccgcgttcac gatcgattcc gcgatacaat attggcgcgg gcgcggcggg atgtggaagg
    14401 gccgttttca aacgatgaag cgggcatgat tgaacaagcg gctgaactgg gctcgggaaa
    14461 ggggcatcgc gacgagaatt ttcccgtcgc gtctgttctg atcgcccccc gtcatcgtcc
    14521 catcattctc gccttctatc gtttcgcgcg cgtcgccgac gatgtcgccg acaatgcggc
    14581 agcacccgcg gacgagaaac tgcggctact cgacggcatg cgccgcacgc tgctgggcga
    14641 attggacacc cagccggagg cgtccgtgct tcgggtcatg ctggcggcgc ggggcttgag
    14701 cccgcagcat gggctcgatc tgatcgaggc attctcacgc gatgtcacaa aactgcgcta
    14761 tcgcgattgg aacgatctga tggattattg ccacgtatcg gcgatgccgg ttggccggtt
    14821 cgtgctcgat gtccatggcg agccgcgcac gctatggcag gcttgcgacg cgctttgcgc
    14881 ggccttgcag atcatcaatc atcttcagga ttgcgcgaag gattatcgcg agctgaaccg
    14941 ggtctacatc ccgctggacg ggtttgccgc cgcaggccta ggaccggaga gtctgaatga
    15001 gccgcgcgcg tccgcaagcc ttcgcgggct tatcgccggt cttgcaaagc ggacatccga
    15061 acttctggtg caatcgcgga atttcgccgc cggcgtgcgc gactccaggc tggcattcga
    15121 aatcagcgtc atccaggttc tggcggagga cctctgccgc cgcctgattc aacgcgaccc
    15181 gctctgcgag cgcgtgcatc atcgcaaaag cgaaatcgcg gccctggccg cgttagccgc
    15241 atggcgcttc ggtgcgggcc gcctgcgccg gcggcaggtt gacatcgtca ttgccgatga
    15301 gcggcgctag cggcgtgcaa gccgggggcc cgcaaagcgg gttcgatccg cgcgccgcgc
    15361 agacgcgcgc gtccggcagc tctttctatg ccggcatgcg ccttctgccg aaggccgagc
    15421 gtgacgcgat gttcgcgatc tacgcgttca gccgcgcggt ggacgacatc gccgatgaag
    15481 gccatgacag ccgcgcgtcg cgtcatgccg cgctcgacga ttggcgtcgc gacatcgaac
    15541 aactttatgc cggctcgccc acaaagcgcg tggaattcct ggttccggtc gtgcgcgttt
    15601 atggcctgcg caaagaggat ttcctcgcga tcatcgatgg catggacatg gatgtcgccg
    15661 aagacatccg cgcgcccgat ctcgccatgc tcgatcttta ttgcgaccgt gtggcgagcg
    15721 cggtcggccg gctttccgtg aagatattcg gaatggacga tgcgcccggt tttaagctcg
    15781 cccatcattt ggggcgcgcg ctgcagatca ccaatatcct gcgcgatctc gatgaggatg
    15841 cggcggccgg gcggctgtat ctgccgcgcg aatttctcgc cgaggccggt atcggggccc
    15901 gcgatccctc tgctgtcatc gccaacactt ggattgacag agcctgccgc aggttggcgg
    15961 cc
//
LOCUS       DASYEG010000153         8997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_5123312, whole genome shotgun sequence.
ACCESSION   DASYEG010000153 DASYEG010000000
VERSION     DASYEG010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 8997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8997
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5123312"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            366..1952
                     /locus_tag="VGM72_07020"
     CDS             366..1952
                     /locus_tag="VGM72_07020"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY4265052.1"
                     /translation="MATNMIRQDETHTDKMDRVRIFDTTLRDGEQSPGAAMTHEEKLE
                     VAALLDTMGIDIIEAGFPIASKGDFEAVRAITGLVKNAGVCGLARAGFKDIDAAGEAL
                     KGARRPRIHTFISTSPVHMKHKLQMGPNAVLDAVGASVTRARSFTDDVQWSAEDATRT
                     ERDFLCRCVELAIRSGATTINIPDTVGYSTPQEYFALIAMLRESVPGAEKVVFSTHCH
                     NDLGLAVANSLAGVLAGARQVECTINGIGERAGNAALEEIVMALKVRRDIMPFRTDVE
                     TTMLSRASKLVSNVTGFPVQYNKAIVGKNAFSHESGIHQDGMLKNVETYEIMRPEDVG
                     VKETSLILGKLSGRHAFRDKLKTLGYELSDSALEDAFRRFKDLADKKKHIFDEDIVAL
                     VDDSVLRGHDPIQVHALRIAAGKDVTATAELSLIVDGDVRSGVASGDGPVDAIFNAIR
                     QLYPHAASLQLFQVSAVTEGTDAQAKVSVRLEEEGRTVTGRGADTDTMVASAYAYVNA
                     LNKLLVKRLKTAPESLTIAR"
     gene            2035..3075
                     /locus_tag="VGM72_07025"
     CDS             2035..3075
                     /locus_tag="VGM72_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010442888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY4265053.1"
                     /translation="MFGSLLGMLSSDMAIDLGTANTLVYVKGRGIVLNEPSVVAIMSK
                     GGKKQVLAVGNDAKQMLGRTPGNIEAIRPMRDGVIADFEVAEEMIKHFIRKVHNRRSF
                     ANPMIIVCVPSGSTAVERRAIQESALSAGARRVFLIEEPMAAAIGAGLPVNEPTGSMV
                     VDIGGGTTEVAVLSLGGIVYSRSVRVGGDKMDEAIIAYIRRHQNLLIGEATAERIKKE
                     VGTAAQPTDGGGTTMEIKGRDLLNGVPKEITITQRQIAEALTEPVSAIVEAVKVALEA
                     TPPELAADIVDKGIVLTGGGSLLANLDQVLRDETGLPVSIADDALSCVALGTGRTLEN
                     AKNMRHMLSTAF"
     gene            3161..4105
                     /gene="mreC"
                     /locus_tag="VGM72_07030"
     CDS             3161..4105
                     /gene="mreC"
                     /locus_tag="VGM72_07030"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY4265054.1"
                     /translation="MIVCAVSAVVIAFLGKADATLFDRARATLSDLTEPALSQVRLPL
                     TAVQGWIANTGTVFTVFQDNIQLERENAELRKWQNVALSLEAKMHRYEILLNAVHDSD
                     PASVTARVIGEATRPFIKSLILNAGADQQVKKGQAVVDERGLLGRVYVTGEKTSWVIL
                     LTDLNSRVPVVIEPYHRRAILIGDNTPAPQLELDVSDGPVKAGDRVVSTGDGGLLPPD
                     LPIGTVMQEGSALRVVLFARPGTSDFVRIMNYSLPDPPIATDSVPAATTTAAEEPKVP
                     PATVTAGITARPAPPAMPLQSRNPDVPRFDGQIEEQDR"
     gene            4102..4647
                     /locus_tag="VGM72_07035"
     CDS             4102..4647
                     /locus_tag="VGM72_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265055.1"
                     /translation="MSSTSNVVELLFHHPLRLLASLIPSVLAILLVLIVNMPVSFTGG
                     LLPAPALALACIYFWVLVRPDLMPPFVVLMLGLMEDLLSGGPPGLWAAGFLAAFWLTD
                     RERETFAGLTGAGALVGFAGAMLFGASVAYGLDALVYLRLPPLPPLLLESVSTVMFYP
                     LIVISMAWFHKRVVGPMRSGA"
     gene            4660..6543
                     /gene="mrdA"
                     /locus_tag="VGM72_07040"
     CDS             4660..6543
                     /gene="mrdA"
                     /locus_tag="VGM72_07040"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY4265056.1"
                     /translation="MALEGKNKSRYAGFSRRAFLLSGGMTAVFGVLAARLYQLQIVDG
                     DRYLTEAEENRISLRLLTPPRGRIFDRFGVPLANSRRNYRVLLVPEQTRQGVRPALDA
                     LGQVIALDDHVRQRVLKEAAGNKPFMPIVVAENLSWDEFARLNLDLPYLPGIQPDVGE
                     TRGYPYAEELSHVLGYVAAVSPEDKAKDESGDPLLDLPGFRLGKRGVEKTFDAEIRGR
                     AGADRVEVNAYGRVIRELDRQPGEAGEDVYLTIDQELQSFLYSRLKDDSAGAAVMDVE
                     TGDLLALVSTPGFDPNAFNVGVTQEQWKALIQDDHKPLINKPLAGFYPPGSTFKTVTA
                     LAALDAGVITPDTVFNCSGQFTLGSHAFHCWKKGGHGSINLRQGLKVSCDCYFYQVAL
                     KLGIDALQAGARKLGLGAVTGIEIPGERAGFIPDRAWKQKTFKEPWQLGETLVAGIGQ
                     GYILTTPLQLCVLAARIAGGKEVSPRLVHSLGATKQPRPPVEPVGFSPDAIDAVRQAM
                     IAVANEPGGTAYGWRITDPAFAMAGKTGTAQVRVISAEERATGIRSNESLPWKLRDHG
                     IFIGFAPVDKPRYACAVLIEHGGLNSHPQVALARDALLLAQKRDILGRPPSYPVNSAD
                     ASL"
     gene            6540..7694
                     /gene="rodA"
                     /locus_tag="VGM72_07045"
     CDS             6540..7694
                     /gene="rodA"
                     /locus_tag="VGM72_07045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEY4265057.1"
                     /translation="MSVHSYSNSARVVPLRHKLGEINWTLVTLITLIACSGFLMLYSA
                     AGGSFSPWAARQMIRFCFGFLILVAVACVDLRVWMGLAYPAYLFSLLLLVAVEVAGRV
                     GLGAQRWIEFGPLALQPSELMKIALVLALARYLHGLEPGAISRPFRLLVPVAMIAAPV
                     VLVLMQPNLGTATLLALCGALLLFLAGLSWYWIAPTVAGVAIAIPMAWEFALHDYQKA
                     RVMTFLNPDTDALGAGWNITQAKIALGSGGIIGKGFLMGTQSRLNFLPEKETDFIFTM
                     IGEEFGFLGTIALLALFVTVIYYGIRIAMRSRSQFGRLLAMGITVNFFLYIMINGLMV
                     MGLIPVVGIPMPLISYGGTAMMTVMFGFGLLMSVHVHRRVDVPRHSSGMI"
     gene            7779..7854
                     /locus_tag="VGM72_07050"
     tRNA            7779..7854
                     /locus_tag="VGM72_07050"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7812..7814,aa:Lys,seq:ctt)
     gene            complement(7912..>8997)
                     /locus_tag="VGM72_07055"
     CDS             complement(7912..>8997)
                     /locus_tag="VGM72_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY4265058.1"
                     /translation="PEALGTDYSTAKSRCDNVLNPQFDAWRTKGLVGANEDRPAVGTF
                     DWLVFVYKSSPKYTNRPKVTQKNYDAALRLVSKHTLKDGRTFGSLSLTSISPGAVDRL
                     HDKLMAKPGGGERGRTADGAMRVCRRAWNVAWRDKRDVVPVSNPFAKMDLHHKAKQTR
                     PVTHAELTSFVKAADAAGEASLGTAAMIAFFWLQREIDIISRLLWNHYRPADNPDIVR
                     IFHHKTREPVDLPLVDDDGTELWPELTARLDAAAKNGTLIITRDKLDAKRKVHLPWAP
                     DYFRHRVAAVRADAGIDADVKFMGLRHGGNTEGADAELSDAQLRALSGHKTVSMVQLY
                     AKASMKQRKVGARKRLESRTKTGGMSE"
BASE COUNT         1619 a         2726 c         2836 g         1816 t
ORIGIN      
        1 ggttctttcc ttgcctggct cagaacaaag cggcataaat ggctgtggat agataggaat
       61 cttctcgtat ttacgggtgg ttaggattat tcgtaaacaa tcatgaacgg atccgttgag
      121 gaacgatttt tccgatgacg aacgaaaggc aaagcgcaat cgcaggaagc atctccgctt
      181 cccgcacgcg cctgtcttcg tcatcgggcg atctttcccg cgtttcgctt tgctgcgggc
      241 tccttctcct tagctgcccc taggcgccgg cagttttttc cgatttcgga aacgggcgtc
      301 tgggggatca tcagcatacg gatgaatgat gaaccgcctc acggcgagcg aaaggatttt
      361 cggctatggc aacgaatatg atacggcagg atgaaacgca cacggacaaa atggaccgtg
      421 tacggatttt tgacacgaca ttgcgggacg gtgaacagtc gccgggggcg gcgatgacgc
      481 atgaggagaa gctggaggtc gcggctctcc tcgataccat ggggatcgac atcatcgagg
      541 cgggatttcc catcgcctcg aagggcgatt tcgaggcggt gcgcgccatc accggactcg
      601 tgaagaacgc aggcgtctgc ggtctggcgc gggccggctt caaggacatc gacgccgcgg
      661 gcgaagccct caaaggcgcg cgccgcccgc gcatccacac gttcatctcc accagtcccg
      721 ttcacatgaa gcacaaattg cagatgggac ccaatgcggt gctcgatgcg gtgggcgcct
      781 cggtcacgcg cgcgcgcagt ttcaccgacg acgtgcaatg gtccgcggaa gacgcgacgc
      841 gcacggagcg cgattttctc tgccgctgcg tggagctggc gattcgctcc ggcgccacaa
      901 cgatcaacat ccccgacact gtgggttatt cgacgccgca ggaatatttc gcactcatcg
      961 cgatgcttcg cgaaagcgtg ccgggcgcgg agaaagttgt gttttccacg cattgtcaca
     1021 atgatttggg cttggcggtg gcaaactcgc tggcgggcgt cctggccggc gcacgccagg
     1081 tcgaatgcac catcaacgga atcggtgagc gtgccggaaa cgcggcgctg gaggaaatcg
     1141 tcatggcgct caaggtccgc cgcgacatca tgccgttcag gaccgatgtc gagacgacga
     1201 tgctgtcgcg cgcttcaaag ctggtttcca acgtcaccgg cttcccggtc cagtacaaca
     1261 aggccatcgt cggcaagaat gccttcagcc acgaatcggg tatccatcag gacggaatgc
     1321 tgaagaacgt ggagacctac gagatcatgc gccccgaaga tgtcggggtg aaggagacct
     1381 cgctgatcct gggcaagctc tccggccgcc acgccttccg cgacaagctg aagacgttgg
     1441 gctatgagct ctccgacagc gcgttggaag acgccttccg ccgctttaag gacctggctg
     1501 acaagaagaa gcacattttc gatgaggata tcgtcgcgct ggtcgacgat tcggtgctgc
     1561 gcggccacga ccctatccag gtccatgccc tgaggatcgc cgccgggaag gatgtcaccg
     1621 ccacggccga attgtcgctc atcgtcgatg gcgatgtgcg ttcgggtgtg gcgagcggcg
     1681 atgggcccgt ggacgccatc ttcaacgcga taaggcagct ttatccgcat gccgcatcgc
     1741 tgcagctctt ccaggtgagc gcggtgaccg aaggcaccga cgcccaggcc aaggtcagcg
     1801 tgcgcctcga agaggagggc cgcaccgtca ccggccgcgg tgcggacacg gacacgatgg
     1861 tggcgtcggc ctacgcctat gtaaacgccc ttaacaaact cttagtaaaa agactgaaaa
     1921 ctgcacccga atcgttaacg attgcgcgat aaattgggca aagccccgcc aaaaggcggg
     1981 gcatcccttg ggattaatgt ctgaacctcc ttcgggggga gtaacggaaa acagatgttc
     2041 gggagtttgt taggcatgtt gtccagcgac atggcgatcg atctggggac ggcgaatacg
     2101 ctcgtttacg tcaagggccg ggggatcgtt ctcaacgaac cctcggtcgt cgcgatcatg
     2161 tccaaaggcg gcaagaagca ggtgctcgcg gtcggcaacg acgccaagca gatgctcgga
     2221 cgcacgccgg ggaatatcga ggcgatccgc ccgatgcgcg acggcgtgat cgccgatttc
     2281 gaagtcgccg aggaaatgat caagcacttc atccgcaagg tgcacaatcg gcgttccttc
     2341 gccaatccga tgatcatcgt ttgcgtgccg tcgggttcga ccgcggtgga gcgccgcgcg
     2401 attcaggaat cggcgctatc cgccggggca cggcgcgtgt tcctgatcga ggagccgatg
     2461 gcggcggcga tcggagcagg gctgccggtg aacgagccaa cgggatcgat ggtcgtggac
     2521 atcggcggcg gcaccaccga agtggcggtg ctttcccttg gcggcatcgt ctattcgcgc
     2581 tccgtgcgcg tgggcggcga caagatggat gaggcgatca tcgcctatat ccgccgtcat
     2641 cagaatctgc tgatcggcga agccaccgcc gagcgcatca aaaaagaggt cggcaccgcg
     2701 gcgcaaccca ccgatggcgg cggcaccacg atggagatca agggccgtga tctgctcaac
     2761 ggcgtgccga aggaaatcac catcacccag cgccagatcg ccgaagcgct gaccgagccg
     2821 gtcagcgcca ttgtcgaagc ggtgaaagtg gcattggaag caacgccgcc ggaactcgca
     2881 gccgatatcg tggataaggg gatcgtgctg acaggcggag ggtcgctgct cgccaatctc
     2941 gatcaggttt tgcgtgacga gaccggcttg ccggtttcca tcgccgacga tgcgctctcc
     3001 tgcgtcgcgc tcggcaccgg acgcacgctc gagaacgcga agaacatgcg ccacatgctc
     3061 tctacggcct tctgaacgcg gcgatggaga caataggacg ataagaatgg cgggtttggg
     3121 ctggagactg acgcgaggcc ggggagcaaa acagctctcc ttgatcgtct gcgctgtttc
     3181 ggccgtcgtc attgcgtttc tgggaaaggc cgatgcgacc ctgttcgatc gcgcccgcgc
     3241 cacgctgtcg gatcttaccg aacccgcact gagccaggtg cgcctgccgc tgaccgcggt
     3301 ccagggctgg atcgcgaata ccggaacggt cttcaccgtc ttccaggaca atatccaact
     3361 cgaacgggaa aacgccgaac tgcgcaaatg gcagaacgtc gcgctgtcgc tcgaagccaa
     3421 gatgcatcgc tatgagattc tgctgaatgc ggtgcatgat tccgatcccg cctccgtcac
     3481 cgcgcgggtc attggggagg cgacacggcc attcatcaaa tccctgatcc tgaatgcggg
     3541 cgccgatcag caggtgaaaa agggccaggc cgtcgtcgac gagcgcggtc ttctggggcg
     3601 tgtctatgtg acgggagaaa aaacctcctg ggtgattctc ctgaccgatc tcaacagccg
     3661 cgtgccggtc gtgatcgaac cgtatcaccg ccgcgcaatt ctgatcggtg acaacacgcc
     3721 ggcgccgcaa ttggaactgg atgtgagcga tggtcccgtc aaggccggcg accgggtggt
     3781 ttcgaccggc gacggcggat tgctgccgcc cgatctgccc atcggcacgg tgatgcagga
     3841 aggcagcgcg ctgcgcgtcg tgctgttcgc gcggcccggc accagcgatt tcgtgcgcat
     3901 catgaactac agcctgccgg atccgccgat tgccaccgat tccgtgccgg ccgcgacgac
     3961 gaccgctgcg gaggaaccca aggttcctcc ggcgaccgtg accgcgggca ttaccgcgcg
     4021 cccagcgcca ccggccatgc cgctgcaatc gcgcaatccc gacgtgcctc gtttcgatgg
     4081 ccaaatcgag gaacaggacc ggtgagttcg acaagcaatg tggtggaatt gctgttccat
     4141 catccgctgc gcctgctggc ttcgctcatc ccgtcggtgc tggccatcct gcttgtcctc
     4201 atcgtcaaca tgccggtctc gttcaccggg ggtcttcttc ccgcgcccgc gctggcgctc
     4261 gcctgcatct atttctgggt gctggtccgg cccgatctca tgcccccttt cgtcgtgctc
     4321 atgctggggc tgatggagga tctcctgtcc ggcggtccgc cgggtttgtg ggcggccggt
     4381 ttcctggcgg ccttttggct gaccgaccgc gagcgcgaaa cattcgcggg acttacaggc
     4441 gccggcgcgc tggtgggttt cgcaggtgcg atgctgttcg gagcttcggt cgcctatggg
     4501 ttggacgcgc tcgtctattt gcggttgccg cccttgccgc cgctgctgct cgaaagcgtc
     4561 agcaccgtga tgttctatcc gctcatcgtg atttcgatgg catggtttca taaacgagtc
     4621 gtcggtccga tgcggagtgg cgcgtaggga tttttggcta tggccttgga gggcaagaac
     4681 aagagccggt acgcgggatt ttcgcgtcgc gccttcttgc tgtccggcgg tatgaccgcc
     4741 gtcttcggtg tacttgcggc ccgcctttat cagcttcaga tcgtcgatgg cgaccgctac
     4801 ctcaccgagg ccgaagagaa ccgcatcagt ttgcggctgt tgaccccgcc gcgcgggcgc
     4861 attttcgacc gcttcggggt gccgctcgcc aatagccgcc gcaattatcg cgtgctgctt
     4921 gtgccggagc agacccggca aggtgtgcgt cctgcgctcg acgcgctcgg acaggtcatc
     4981 gccctggacg atcatgtgcg tcagcgcgtg ctgaaggaag cggcgggaaa caagcccttc
     5041 atgcccatcg tggtcgcaga gaatctctct tgggacgaat tcgcgcggct caatctggac
     5101 cttccctatc tgccgggcat tcagcccgat gtgggtgaaa cccgcggtta tccgtatgcg
     5161 gaagaactct ctcatgtcct cggttatgtc gcggctgtct cgcccgagga caaggcgaag
     5221 gacgaatccg gcgatcctct gctcgatctg ccgggatttc ggctgggcaa gcgcggcgtc
     5281 gagaagacgt tcgacgccga aatccgcggc cgcgccggcg cggaccgggt cgaggtcaac
     5341 gcctatggcc gtgtcatccg cgaactcgac cgccagccgg gcgaagccgg cgaagacgtc
     5401 tatctcacca tcgatcagga attgcagagc ttcctctatt cccggctgaa ggacgacagc
     5461 gccggggctg cggtgatgga tgtggagacg ggcgatctcc tggcgctggt ttccacgccg
     5521 ggtttcgatc ccaacgcctt caatgtcggg gtcacgcagg aacagtggaa agcgctgatt
     5581 caggacgacc ataagccgct catcaacaag ccgctggcgg gattttatcc gccggggtcc
     5641 accttcaaga ccgtcacggc gctcgcggca ctcgatgccg gcgtcatcac gccggacacg
     5701 gttttcaact gctcgggcca gttcaccctt ggctctcacg ccttccattg ctggaaaaag
     5761 ggcggccatg gcagcatcaa tctgcgccag ggtctgaaag tctcctgcga ttgctatttc
     5821 tatcaggtag cgctgaagct cggcatcgat gcgctgcagg ccggtgcccg caagctcggg
     5881 ctgggcgcgg tcacgggcat cgagattcca ggcgaacgcg ccgggttcat tcccgaccgg
     5941 gcatggaagc agaagacgtt caaggagccc tggcaattgg gcgaaaccct ggtcgcgggt
     6001 atcggtcagg gctacatcct gacgacaccg ctgcagcttt gcgtgctggc cgcgcgcatc
     6061 gcgggcggca aggaggtaag cccgcgcctt gtgcattcgc tgggtgccac aaaacagccg
     6121 cggcctccgg tcgagccggt gggtttctca cccgatgcca tcgacgcggt acgtcaggcc
     6181 atgatcgcgg tcgccaacga accgggcggg actgcctatg gctggcggat cacagatccc
     6241 gcctttgcca tggctggaaa gacgggcacc gcgcaggtgc gtgtgatcag cgccgaggag
     6301 cgggccaccg gtatccggag caatgagagc ctgccctgga agctgcgcga tcatgggatc
     6361 ttcatcggct tcgcgccggt cgacaaaccg cgctatgcct gtgcggtgct gatcgaacat
     6421 ggcggcctga actcccatcc gcaggtggcg ctcgcgcgcg atgcgctgct gctggcgcag
     6481 aagcgcgaca tcctggggcg gcccccgtcc tatccggtca attcggcgga tgcatccctg
     6541 tgagcgtgca ttcctatagt aacagcgcgc gcgtggtgcc gttgcgccat aagctcggcg
     6601 agatcaattg gactctggtg acgctgatca cgctgatcgc ctgctccgga ttcctgatgc
     6661 tctattcggc ggcgggcggg agtttctcgc cctgggcggc gcggcagatg atccgcttct
     6721 gctttgggtt tctcatcctt gtcgccgtcg cctgcgtgga tttgcgggtg tggatggggc
     6781 tcgcctatcc ggcttacctg ttttcgctgc tgcttctggt ggcggtggag gtcgcgggcc
     6841 gtgtcggtct gggcgcgcag cgctggatcg agttcgggcc cttggcgctg cagccctccg
     6901 agctgatgaa gatcgcgctg gtgcttgcat tagcgcgcta tctgcacgga ctcgaacccg
     6961 gcgcgatttc gcgtcccttc cgtctgctgg tgccggtggc gatgatcgcg gcgcccgtcg
     7021 tgctggtgct gatgcagccc aatctcggca ctgcgacatt gctcgcgctg tgcggggcgc
     7081 tgctgctgtt tctggcgggg ctctcctggt actggatcgc gccgaccgtc gcgggcgttg
     7141 ccatcgccat tccgatggcc tgggaattcg cgctgcacga ttatcagaag gcgcgcgtga
     7201 tgaccttcct caatcccgat accgacgcgc tgggggcggg gtggaacatc acccaggcca
     7261 agatcgcgct cggctccggc ggcatcatcg gcaaggggtt cctgatgggg acgcagagcc
     7321 ggctgaactt ccttcccgaa aaagagacgg atttcatctt caccatgatc ggcgaggagt
     7381 tcggtttcct cgggaccatc gcgctgctcg cgctgttcgt gacggtcatc tattacggca
     7441 tccgcatcgc gatgcgctcg cgcagccaat tcgggcggct tctcgccatg ggcatcaccg
     7501 tcaatttctt cctttacatc atgatcaacg ggttgatggt gatgggcctc attcccgtgg
     7561 tcggcatccc catgccgctg atctcctatg gcggcacggc gatgatgacc gtgatgttcg
     7621 gttttggcct tctgatgagc gtgcatgtcc accggcgcgt cgatgtgccc cgccattcca
     7681 gtggaatgat ctgaaccttt tccctgcgct actcgacagg tgaggagggc tttgctaaga
     7741 cccgccctcc cgccgggtct gaaatggccg ggcggtacgg gtgtatagct caggtggtag
     7801 agcagatgac tcttaatcat cgggtcgcag gttcgagtcc tgctacaccc accaattgat
     7861 tcaatcactt agcctatgga cgaaaagtca ttccgacatt tcgtcaccgg ctcattccga
     7921 cattccccct gttttcgttc tcgattccag tctctttcga gcgccgacct tgcgctgttt
     7981 catacttgcc ttcgcgtaga gctggaccat agacacagtc ttgtgtccgc ttagggcgcg
     8041 cagctgagcg tcgcttagct cggcatccgc gccttctgta tttccgccat ggcgcagccc
     8101 catgaatttc acatcggcat caatacccgc atcggcccga acagcggcga ctctgtgccg
     8161 gaaatagtcc ggtgcccatg gcaaatggac cttccgcttc gcatcgagct tatcgcgcgt
     8221 gatgatgagt gtgccattct tcgcagcggc atcgagacgc gccgtcagtt ccggccacaa
     8281 ttccgtgccg tcatcgtcga ccaatgggag atctaccggc tccctggttt tgtgatgaaa
     8341 tatgcgaacg atgtcggggt tgtcggctgg gcgatagtga ttccacagaa gccggctgat
     8401 gatatcgatc tcacgctgca gccagaagaa cgcgatcatt gcggcggtgc cgagcgatgc
     8461 ttcgccggcg gcatccgcgg ctttgacgaa actggtcaac tcggcatgcg tgaccggccg
     8521 ggtctgtttg gccttgtggt gcagatccat cttggcgaat ggattcgaca caggaacaac
     8581 gtcgcgcttg tcgcgccaag cgacgttcca ggcgcgccgg caaacccgca ttgcaccatc
     8641 ggcggtgcgt ccgcgctcac cgccccccgg cttcgccatc agcttgtcat ggaggcgatc
     8701 gacggcgccg ggcgaaatgc tggtaagcga taagcttccg aacgtcctgc cgtccttgag
     8761 cgtgtgtttc gacaccaacc ggagcgcagc atcgtaattc ttctgcgtga ccttcggccg
     8821 gttcgtgtat ttcggcgacg acttgtagac gaagacgagc caatcgaacg tgccaaccgc
     8881 ggggcggtct tcattcgcgc cgaccaaccc cttggtgcgc cacgcgtcga attgcggatt
     8941 gaggacgtta tcgcaacggc ttttggccgt gctgtaatcg gtcccgagcg cctcggg
//
LOCUS       DASYEG010000154         7552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3093175, whole genome shotgun sequence.
ACCESSION   DASYEG010000154 DASYEG010000000
VERSION     DASYEG010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3093175"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VGM72_07060"
     CDS             complement(<1..503)
                     /locus_tag="VGM72_07060"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEY4265059.1"
                     /translation="MTSTALVDFQVKRLLESFAPIIPRDRPAALIDFPDSTNCGDHAI
                     WLGEKVLLSRLGVPIAYECSPQTYDREALAARIGSGPILLQGGGNFGDRYPAHHEFRL
                     RVLQDFPKNRIVFFPQQVTFLDNDYLQRTADFLKEHAGVTLFARGVVAQQMFTRYFGP
                     IARVEPAP"
     gene            683..1588
                     /locus_tag="VGM72_07065"
     CDS             683..1588
                     /locus_tag="VGM72_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEY4265060.1"
                     /translation="MQALFIGQTYIDVTFIADRIPTGDEKDVAHDYAISFGGNAVTAA
                     FCCARLGIAPDLLTSLADDWLGRMFLDMAAKYQISVHARKVRRSSLSFVMPKDGKRAI
                     LRLRDDNYLHPFPPLNLEGCRALHLDGHQGDGALHYARACRQAGIFTSLDGGGLRSNT
                     MELLPFIDAAVVSERFCEQLQRPPADVLDLLKQKGCRIGAVTLGERGLLWFDERGQVA
                     IMPALAVPPGKIVDTNGAGDVFHGAYVYSRMMNPDAAWEAHFRFARAASAHAICHLGN
                     EARLPTLADLEEVDRLYQPGAAATA"
     gene            complement(1590..4715)
                     /locus_tag="VGM72_07070"
     CDS             complement(1590..4715)
                     /locus_tag="VGM72_07070"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY4265061.1"
                     /translation="MSFTDIFVKKPVLATVVSLIILVIGLRAALSLPVRQYPRTENAV
                     VTITTAYFGADPDVVAGFITQPLENVIAQANGIDYITSTSANSLSTITVNLRLNYDYN
                     RAVTEITSKISSVLNRLPPQSQQPALQVQVGQAVSAMYLGFRSNVLQPNQITDYLTRV
                     VQPKLQAVAGVQTVNILGARNFALRAWLDPAKLAAYGLTATDVNTALATNDYISSLGN
                     TKGQMVQVNLTASTDLHSLDDFRNLIIKQANGAIVRLSDVATVVLGAEDYNTNISYNG
                     QNSIAMSIDIAPDANLVDVMKQIQIVFPQIQSQLPQGLDAAILYDASQFVNSAISEVE
                     STIVKTLLIVMLVVFVFLGSIRSVVIPIVAIPLSLIGAFIMMLVLGFSINLLSLLAMV
                     LAIGLVVDDAIIVVENVHRHINEGARPFDAALQAARELGGPIIAMTAVLIAVYLPIAF
                     QGGLTGALFVEFAFTLVGTIIISTIVALTLSPMMCSKLLKPHDENDRGLNARITRFID
                     RRFERLHGFYERRLHGALNYVPVTFVFAIIVILSTGALWMTTQQELAPQEDQGFIFAF
                     GPGTPTASVTQYKAFSDAVYDKLKTNQNLDFIFQMDSTSRLIKFFALKPWDQRPENSF
                     QVQQDVQNELIQFPAFNNFFVSQPPPLPSTFGAPVQFVISTTQSFDRLNDSVQTFLTE
                     VRDSKKFFFVDTDLKIDRPEVTVDIDRDKAALMGLSMSDVGNALSAMLGGGYVNYFSM
                     EGRSYRVMAQADQPFRLNADQILNYYIRAANGALIPLSTVANITTKTTPESLAHFQQL
                     NSATINLAPGFGMTQGDALTYLQQVAARTLPEGYIIDYAGTSRQYIQESSGFLTLLLF
                     AAVIIFLVLSALFESFRDPFIILMSVPMSIAGAMAAMHLVGGLHVFFPNMMQFGGATL
                     NIYTLVGIVTLMGLISKHGILIVDVANRLQREGHAKREAVELAAGIRLRPILMTTAAM
                     VLGVVPLLLSTGAGAVARFAMGLVIFSGISIGTLFTLFVVPTFYILIAKDFTRARARA
                     GALQAQPVPGE"
     gene            complement(4725..5900)
                     /locus_tag="VGM72_07075"
     CDS             complement(4725..5900)
                     /locus_tag="VGM72_07075"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY4265062.1"
                     /translation="MNRPERPELMVEEHPPRRRRLWLWLALVAILAIVLFGAIFAFPI
                     IAFKAKGFPAPPPATVSTAKAVLEDWQPAIQIVGSLKPLQGADLSMEVAGIVQEVNFD
                     SGGDVKAGEPIIKLIDADDVAKLHTLEASRDLAQTNYNRDQSQLQRQLISQAAFDVTT
                     ANLKSFQAQVAEQQAIVEKKTLRAPFSGHLGIRLVNPGQFLNPGTVVVTLQALDPIFL
                     DFSLPQQQLNQIKPGLTITAKVDAYPGAAFTGKITTIDPKVDPATRNVMVRATLPNRD
                     HRLLPGMFATGEIVTGQKQRYITLPQAAITFNPYGNVVFLVKPGKDPAGKDILVGQQT
                     VVTTGATRGDQIAVLTGLKENDTVVSAGQAKLQNGAPLLVNNAVTPTNEPNPQPHEQ"
     gene            complement(5897..7384)
                     /locus_tag="VGM72_07080"
     CDS             complement(5897..7384)
                     /locus_tag="VGM72_07080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY4265063.1"
                     /translation="MRDSIRRLLLASTAVAGLTGCTVLGPDFARPQPPMVTGYTADAL
                     PPATVSADTPIAGDAQRFLQDTDVPGRWWDLFGSPLLNDLVEQALRANPDVDAAQATL
                     RQAHENLIAQRGALFPQVNGTAQLGRQQSVTTLDTLDLFNASVSVSYLIDAFGGVQRS
                     IEATGALEENSRFTLEATYLTLTSNVITAAIQEASLTSQIAAIGDIITAQAQELDLLN
                     QQFELGAVARGDVLAQQSQLAATQASLQPIQKQLEQVRNTLVILTGRYPSEGPAPNIQ
                     LADLRLPPDLPLSLPSKLVEQRPDIRASEALLHNASANIGVATANLFPQFTVTGSLTD
                     SAFQVGDFFTGANTGWSLIAGATAPIFRGGTLRAQQRAAYDVFDRSAAQYRGTVLNAF
                     ANVANVLSALQLDAENVKTQLYAEQTAEQSLEITQERFQAGAIAYLSLLDAQRTYQQA
                     RIALVIAQANRFADTVALFQALGGGWWNRQDAPEFHDPTGLARLP"
BASE COUNT         1521 a         2137 c         2381 g         1513 t
ORIGIN      
        1 ggtgccggtt ctacccgcgc gataggaccg aaataacggg tgaacatctg ctgcgcgaca
       61 acgccgcgcg cgaacagcgt gacgccggca tgttctttca ggaaatcagc ggtccgctgc
      121 aagtaatcat tgtcgagaaa cgtgacctgc tgcggaaaga aaacgattcg gttcttcgga
      181 aaatcctgca ggacacgcag gcggaattcg tgatgggcgg gatagcggtc accgaaattt
      241 cccccgccct gcagcaagat gggcccgctc ccgattcggg ccgccagggc ctcgcgatcg
      301 taagtctgcg gcgaacattc ataggcgatt gggacgccaa gacgagaaag aagcaccttc
      361 tcgccaagcc atatggcgtg gtctccacaa ttcgtcgaat ccgggaaatc gatcagcgct
      421 gcgggacggt cacgcgggat gatcggcgcg aaactctcaa gcaaacgctt gacctggaaa
      481 tccacaaggg ccgtgctcgt cattcgccgg tacccgtttc ttctggcgcc ccccgatgtg
      541 cagtttgtca ccgtccgatt ggccggttgc aagccttttc acgctcggaa cgcgatttgc
      601 ccggccccgc acaaaggttt gacgcatttc gcgcgtcaga ccactatcgt catgttcggt
      661 ttcgcatttc aggttgtcat tgatgcaagc ccttttcatc ggtcagactt atatcgatgt
      721 gacgttcatc gccgaccgga taccgacggg tgacgagaag gacgtagcgc atgactacgc
      781 gatctccttc ggaggcaatg ccgttacggc agcgttttgc tgtgcgcggc tcgggatcgc
      841 gcccgatctt ctgacgtcgc tggccgacga ctggctgggg cgcatgtttc tggatatggc
      901 ggcgaaatat caaatatcgg tgcacgcgcg caaagtccgg cgttcgtctc tgtccttcgt
      961 gatgcccaag gacggcaaac gcgcaattct gcggctgcgc gacgacaatt atctgcaccc
     1021 ctttccgccg ctcaatctcg aagggtgccg cgcgcttcat ctcgacgggc atcaggggga
     1081 tggcgcgctg cattacgcga gagcatgcag acaggccggt attttcacct ccttggacgg
     1141 cggtggcctg cgcagcaaca ccatggagct tttgccgttc atagacgcgg cggttgtgtc
     1201 ggaacgattc tgcgagcagt tgcagaggcc gccggctgac gtgctcgatc tcttgaagca
     1261 gaaagggtgc cgcattggtg cggtcacctt aggcgaacgg ggtctgctct ggttcgatga
     1321 gcggggtcag gtcgcgatca tgccggcgct cgcggtgcca ccggggaaaa tcgtcgacac
     1381 caacggcgcg ggcgacgtgt ttcacggcgc ctacgtgtat tcccgcatga tgaatccgga
     1441 tgcagcgtgg gaagcacatt ttcgctttgc gcgcgcggca tccgcgcatg cgatctgcca
     1501 tctcggcaat gaggcacggc tacccacgct cgccgatctg gaggaggtgg ataggctcta
     1561 ccagcccggc gcggccgcga ccgcctgacc tattcgcccg gaaccggttg ggcctggagc
     1621 gcgccggcgc gtgcccgcgc ccgtgtgaaa tccttggcga tcaggatgta gaatgtcggc
     1681 accacgaaca gcgtgaacag cgtgccgatg gaaatgccgc tgaaaatcac caggcccatg
     1741 gcgaagcgcg ccaccgcgcc agcaccggtg gacagcagca gcgggaccac ccccagcacc
     1801 atggccgcgg tggtcatcaa aatcggccgc agccggatgc ctgctgcgag ttcgacggct
     1861 tcgcgtttgg catggccttc gcgttgcagc cggttcgcca catccacgat caatatcccg
     1921 tgcttgctga tgaggcccat tagggtcacg attcccacca gcgtgtagat gttcagggtc
     1981 gcgccgccga actgcatcat gttcgggaag aacacatgca ggcctccgac gagatgcatc
     2041 gcggccatgg ccccggcaat cgacatcggc accgacatca ggatgatgaa gggatcgcgg
     2101 aagctctcga acagcgcgga caggacaagg aaaatgatca ccgccgcgaa cagcagcaat
     2161 gtcagaaagc cgctcgattc ctggatgtat tgccgcgacg tgccggcata atcgatgatg
     2221 tatccctcgg gcagggttcg ggccgcgacc tgctgcagat aggtgagcgc atcgccctgg
     2281 gtcatgccga agcccggggc cagattgatg gtggccgaat tgagctgctg gaaatgcgcc
     2341 agcgattcgg gggtcgtctt ggtggtgatg ttcgccacag tagaaagggg aatcaacgcc
     2401 ccgtttgccg cccggatata ataattgagg atctgatcgg cattgagccg gaagggttga
     2461 tcggcctgcg ccatgacgcg ataggaacgt ccctccatgc tgaaataatt gacgtagccg
     2521 ccgcccagca tcgccgacag cgcattgccg acatcgctca tggaaaggcc catgagggca
     2581 gccttgtcac gatcgatatc caccgtgact tccggcctgt cgatcttgag atcggtgtcg
     2641 acaaagaaga atttcttgct gtcgcgcacc tcggttagga aggtctgcac cgagtcgttc
     2701 aggcgatcga aggattgcgt ggtgctgatg acgaattgca ccggcgcgcc aaaagtgctc
     2761 ggcaagggcg gaggttgcga gacgaaaaaa ttgttgaagg cggggaactg tatcagttca
     2821 ttctgcacgt cctgctgaac ttggaaggaa ttttccgggc gctggtccca gggcttgagg
     2881 gcgaagaatt ttatcagccg ggaggtcgaa tccatctgga agatgaaatc gagattctgg
     2941 ttcgttttca gcttgtcgta gaccgcgtcc gagaacgcct tatattgcgt gaccgacgcc
     3001 gtcggagtgc ccggcccaaa ggcaaaaatg aagccctgat cttcctgtgg cgcgagttcc
     3061 tgctgtgtgg tcatccacag cgccccggtg ctgaggatga cgatgatcgc gaagacaaag
     3121 gtgaccggca cgtaattcag cgcaccatga agccggcgct cgtaaaaacc gtgcaacctc
     3181 tcgaaacgcc ggtcgatgaa gcgcgtgatg cgggcgttca gaccgcggtc gttctcgtca
     3241 tgcggcttca ggagcttgga acacatcatc ggcgaaagcg tcaacgcgac gatggtcgag
     3301 atgatgatcg tgccgaccag cgtgaaggcg aattcgacga atagcgcacc ggtcaagcca
     3361 ccttggaacg cgatcggaag atagaccgcg atcagaaccg ccgtcatcgc gatgatcggc
     3421 ccgccgagct cgcgtgccgc ttgcagcgct gcatcgaacg ggcgcgcgcc ttcgttgatg
     3481 tgccgatgca cattctccac cacgatgatc gcatcgtcca ccacgagtcc gatggcgagc
     3541 accatcgcca gcagcgacag cagattgatg gagaagccca gcaccagcat catgatgaag
     3601 gcgccgatca gcgacaaagg aatggcgacg atcggaatca cgacgctgcg tatcgagcca
     3661 aggaagacga acaccaccag catgacgatg agcagcgtct tgacgatggt gctttccact
     3721 tcgctgatcg ccgagttcac gaattgcgag gcgtcgtaga gaatggcggc atcgagtccc
     3781 tgggggagct gcgattggat ctgcggaaag acgatctgaa tctgcttcat cacgtcgacg
     3841 agattggcgt cgggagctat gtcgatcgac atcgcgatgg aattctggcc gttgtaggaa
     3901 atgttggtgt tgtagtcctc cgcgcccagc acgactgtcg cgacgtcgga gagccggacg
     3961 atggcgccat tggcctgttt gatgatcaga ttgcggaaat catccagcga gtgcaggtcg
     4021 gtcgaggcgg tgaggttaac ctgcaccatc tgccccttgg tattgcccag gctcgagatg
     4081 taatcgttgg ttgccaatgc ggtattgacg tcggtggcgg tcaggccata ggcggcaagc
     4141 ttggccggat cgagccacgc acgcagtgcg aaattgcgtg cgcccaatat gttcacggtc
     4201 tgcacaccgg ccacggcctg taatttgggc tgcaccacgc gtgtcaaata atcggtgatc
     4261 tgattcggct gcaggacatt ggagcggaat ccgagataca tcgccgaaac cgcctggccg
     4321 acctggacct ggagtgcggg ctgctgcgat tggggcggca ggcggttgag caccgaactg
     4381 atcttgctgg tgatctcggt aaccgcacga ttgtaatcgt agttcagcct cagattgacg
     4441 gtgatcgtcg aaaggctgtt ggcgcttgtc gaggtgatgt agtcgatgcc gttggcttgg
     4501 gcgatgacgt tttccaaagg ctgcgtgatg aatccggcga ccacatcggg atcggcgccg
     4561 aaataagcgg tcgttatcgt gaccaccgcg ttttcggtgc gcggatattg ccgtaccggc
     4621 aaggacagcg ccgcgcgcag accgatgacc aggataatca ggctgacgac ggtcgccaga
     4681 accggcttct tcacgaaaat gtcggtgaag ctcatccgtt gcccctattg ttcgtgcggc
     4741 tggggatttg gttcgttcgt cggcgtgacc gcattgttga ccaagagcgg cgcgccgttc
     4801 tgcagctttg cctgaccggc gctgacgacg gtatcgttct ctttcagacc ggtgagcacg
     4861 gcgatctgat cgccacgggt ggcgcccgtg gtcacgacgg tctgctgtcc gacgaggatg
     4921 tccttgccgg ccggatcttt gcccggcttc accagaaaga cgacattgcc ataggggttg
     4981 aaggtaatcg cggcctgcgg cagcgtgatg tagcgctgct tttgaccggt cacgatttca
     5041 cctgttgcga acatgccggg cagcaagcgg tgatcgcgat tgggcagggt ggcgcggacc
     5101 atgacgtttc gcgtcgccgg atcgaccttc ggatcgatcg tggtgatttt cccggtaaag
     5161 gccgcgccgg gatacgcgtc cacttttgcg gtgatcgtca gcccgggttt gatctgattc
     5221 aattgctgct gcggaagaga gaaatccaga aaaataggat cgagcgcctg caaggtcacc
     5281 acgacggtgc ccggattcaa aaactggccg gggttgacca ggcgaatgcc cagatgcccg
     5341 ctgaaaggtg cgcgcagcgt ttttttctca acgatggcct gctgctcggc cacttgcgcc
     5401 tgaaacgatt tgaggttggc cgtcgtgaca tcgaatgcgg cctgactgat gagttgccgc
     5461 tgcaactgcg actgatcgcg attgtaattt gtctgggcca aatcgcgcga ggcttcgagc
     5521 gtatgaagct tggccacatc atcggcgtcg atcagcttta tgatcggctc tccggccttc
     5581 acatcgccgc ccgaatcgaa attcacctcc tgcacgatgc cggccacttc catcgaaaga
     5641 tccgcccctt gcaggggctt gagagaaccg acgatctgga tcgcgggttg ccagtcttcg
     5701 agcacggctt tcgcggtgga gaccgtcgcg ggcggtggcg cgggaaatcc cttggctttg
     5761 aaggcgatga tcggaaacgc gaaaatcgca ccaaacagaa cgatagccag gatggcgacc
     5821 agcgccagcc atagccaaag gcgcctgcgg cgtggtggat gttcttccac catgagttcg
     5881 ggacgttcgg gccggttcat gggagtctcg caaggcctgt gggatcgtga aattcagggg
     5941 catcctgacg gttccaccaa ccgccgccga gggcctgaaa cagcgcaact gtatcggcga
     6001 agcggttggc ctgcgcgatc acgagcgcga ttcgtgcctg ctggtaagtg cgctgcgcgt
     6061 cgagcagcga aagatacgcg atcgctccag cctggaaccg ttcctgcgtg atctcgagac
     6121 tctgctctgc cgtttgttcg gcataaagct gcgttttcac gttttccgcg tcaagctgga
     6181 gcgcggaaag cacattggcg acattggcaa acgcgttcag cacggtgccg cgatattgcg
     6241 cggcggagcg gtcgaacacg tcatacgccg cgcgttgctg cgcgcgcagc gtgccaccgc
     6301 ggaaaatcgg cgccgtggcg cccgcgatca ggctccagcc cgtattggcg ccggtgaaga
     6361 aatcgcccac ctggaaggca ctgtcggtca gactgccggt gacggtgaat tgcgggaaga
     6421 gattggccgt ggcgaccccg atattcgcgc tggcattatg cagtagcgct tcagacgcac
     6481 gaatgtcggg ccgctgctcg accagcttgg atggcaggct cagtggcagg tccggcggca
     6541 atcgcagatc ggcaagctgg atgttcggcg ccgggccttc cgagggataa cggccggtca
     6601 gaatgacgag cgtattgcgc acctgttcca attgtttttg gatcggttgc aggctcgcct
     6661 gcgtggcggc gagctgcgac tgctgcgcca gcacatcgcc gcgcgccacc gcgccgagtt
     6721 cgaattgctg gttcaggaga tctagttcct gggcctgggc ggtgatgatg tccccaatgg
     6781 ccgcgatctg agaggtaagc gatgcttcct ggatcgcagc ggtgatcacg ttcgaagtca
     6841 gtgtgagata ggtggcctcg agagtgaagc gcgagttttc ttccagcgcg ccggtcgctt
     6901 cgatcgaacg ctgcacaccg ccgaaggcat cgatcagata ggacacgctg accgatgcgt
     6961 tgaagagatc cagcgtgtcg agcgttgtga ccgattgctg acgtccaagc tgagccgtcc
     7021 cgttgacctg ggggaacagc gcgccccgct gagcgatgag gttttcatgc gcctgcctca
     7081 gggtcgcctg cgccgcatcg acgtccggat tggcccgcaa cgcctgttcc acgaggtcat
     7141 tcagcaaagg ggaaccgaac aggtcccacc atcggccggg cacatcggtg tcctgaagga
     7201 atcgctgggc atcgccggcg atgggggtgt cggcggaaac agtggccggt ggcagcgcat
     7261 cggccgtata gccggtaacc ataggcggtt ggggccgcgc aaaatcgggg ccaagcacgg
     7321 tgcagcccgt cagtccggcc acagccgtgg aagccaaaag aagtctacga atggaatcgc
     7381 gcacgctaaa agtccctcaa ttgcatccga cctatagccg aaaaatgctt aaccgccgac
     7441 ctaagcctct gcagatgttc cccgccggcg gatgtcgtta tgcgctcgaa aatgtgcctt
     7501 gattttggaa actagttagt tccctttttt ttgtcaatcc atcatactgt gt
//
LOCUS       DASYEG010000155        17498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1062806, whole genome shotgun sequence.
ACCESSION   DASYEG010000155 DASYEG010000000
VERSION     DASYEG010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 17498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17498
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062806"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VGM72_07085"
     CDS             <1..370
                     /locus_tag="VGM72_07085"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4265064.1"
                     /translation="INVTANWRLIRDFDPLLRQSDAGRVLMMSSAVARLNRPYWGPYA
                     ITKAALEMLTFTYAAECNGTKVMANAYNPGPTRTRMRAEAMPGEDPMTLPAPEDLVPS
                     ILELLSPASTKNGELVAFRR"
     gene            complement(373..1728)
                     /gene="der"
                     /locus_tag="VGM72_07090"
     CDS             complement(373..1728)
                     /gene="der"
                     /locus_tag="VGM72_07090"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY4265065.1"
                     /translation="MPLTVAIVGRPNVGKSTLFNRLAGKRLAIVDDTPGVTRDRAFAA
                     SNFAGLDLALIDTAGFDKGAPESLVARMATQTGTAIRDADVCLFVVDARAGLTAGDEI
                     VAETLRRSGKPVILAANKCEGTAAEAGANEAYRLGFGEPIAVSAEHGLGLAELRDALS
                     VYFPEIPPSEEATESPEAEMENRPLRLAIVGRPNVGKSSLLNRLIGEDRSLTGPEAGI
                     TRDAVLAQWRHEERDILLHDTAGLRKKARIDERLEKLSVNSTLNAVRFADCVIVVMDA
                     NEALEKQDLAIADLIAREGRAIVIAVNKWDLVADRGAAFKDLRERVDRFLPQIAGAPI
                     VGVSALTGEGMERLMPTVFEAERIWNLRVSTHAVNRFLEEALSRHPPPAVHGRRIRIR
                     YMTQAKARPPTFVLFGNQLKALPDSYLRYLVNAMRGAFQMPGTPVRLNLRENKNPYSD
                     K"
     gene            complement(1756..3153)
                     /locus_tag="VGM72_07095"
     CDS             complement(1756..3153)
                     /locus_tag="VGM72_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY4265066.1"
                     /translation="MKSRIASSLVLLLVAGGILGGCADTYNTVADWFDRTKKTKIQGE
                     RISIIASEQQLSSDPMLAATKMDLPPPKKNADWPQPGGTPDNVTGNLMADGPLEQVWS
                     VSAGKGTDDDSRLTAPPIVAGGLIYVLDAETHVFAFDAQTGKPVWDKSLAPESTDTSS
                     WYNIFGSSNSIKPSKGFGGGIAFDSGKLFAATGFGNVTALDAKTGKQLWSVNTVVPVH
                     SAPVVVDGRVYVITQENETQALDANDGHRLWDHHGTVESAAILSSASVGVSGDTVVVP
                     YSSGELYALRAQNGTPSWTDTLTRSGNTTSLTIINDIAGRPVIDRNMVFAVSHSGTLA
                     AINFRSGSRAWTRNIAGIQTPLVAGEYIFIVSTDGQVVCMNRGDGRVRWTAALPVFED
                     PKGKRDPIVWTGPLLVSNFLVLIASDGKATLLSPFTGQKLGDTAIPDGTFIPPIVANG
                     MMYVLTNEAQLVALR"
     gene            complement(3150..3938)
                     /locus_tag="VGM72_07100"
     CDS             complement(3150..3938)
                     /locus_tag="VGM72_07100"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4265067.1"
                     /translation="MSDIFQEVEEDVRRERYEQIWKKYGNYIMIAAALLVAAVAGYQA
                     WQRYDLTQRQKISDRYREASQSAQTGNLPKAETDFAALAKDAPSGYATLAKFHLAGAY
                     LAQGKRDAAVALLRELTASSDSIVSSTARLRLAWTDADASPKADIVNLLQPLTAMDNP
                     WRFAAAEVLAYADLKNGARSQALAEYQKLAQESEAPASLRQRASGIAEYLGANPDAAI
                     SGPTPPSLPSLGAPAAASSLPAAAAPVASPAPGSGVPSGQGTKP"
     gene            complement(4066..4899)
                     /gene="panB"
                     /locus_tag="VGM72_07105"
     CDS             complement(4066..4899)
                     /gene="panB"
                     /locus_tag="VGM72_07105"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEY4265068.1"
                     /translation="MSAQAETDRITVPQIRARKGGTPIACLTCYHAHTARLLDNHVDV
                     MLVGDSLGMVMHGMETTLGVTLELMILHCQAVMRGSKRALVAIDMPFGSYEESRETAF
                     RNASRAMKETGCTAVKLEGGVRMAETIHYLTERGIPVMAHIGLTPQMVQVMGGFRTQG
                     RDEAEWPALEADAKAISDAGAFSVVIEGIAEPLAAKITKSIPIPTIGIGASPECDGQI
                     LVMEDMLGLNPHPPRFVREYAHLGPDIEAAVTSFVADVRSRRFPGKENVYAMRKGSKA
                     I"
     gene            complement(5009..5593)
                     /gene="rplI"
                     /locus_tag="VGM72_07110"
     CDS             complement(5009..5593)
                     /gene="rplI"
                     /locus_tag="VGM72_07110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY4265069.1"
                     /translation="MQIVLLERVEKLGQIGDVVKVKDGFARNFLLPKKKALRATKANL
                     SYFDSQRVQLEARNLEQRKEAEKIAEKLQGKSFVLLRQAGDRGQLYGSVSPRDIADAT
                     TAGGFSIQRTQVPIDKAIKTIGLHEISVLLHPEVRVQVTINVARTEDEAERQARGEDV
                     LTEQTEAEEARTNAEALFEEGAGPKAEGEEETPE"
     gene            complement(5605..5850)
                     /gene="rpsR"
                     /locus_tag="VGM72_07115"
     CDS             complement(5605..5850)
                     /gene="rpsR"
                     /locus_tag="VGM72_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEY4265070.1"
                     /translation="MSAQPPLARRPFFRRRKTCPFSGSNAPKIDYKDVKLLQRYVSER
                     GKIVPSRITAVCAKKQRELSKAIKRARFMALIPYLVK"
     gene            complement(5847..6245)
                     /gene="rpsF"
                     /locus_tag="VGM72_07120"
     CDS             complement(5847..6245)
                     /gene="rpsF"
                     /locus_tag="VGM72_07120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY4265071.1"
                     /translation="MALYEHMLIARQDISAQAVDALATHLKTIIETEGGKVEKQEYWG
                     LRTLAYRIKKNRKGHYVLLNLNAPANAISELERQLKINEDVLRFITVRVDAFEQSNSS
                     RKREREAGAGGREGERRPREDGAETEGAMV"
     gene            6600..8678
                     /locus_tag="VGM72_07125"
     CDS             6600..8678
                     /locus_tag="VGM72_07125"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEY4265072.1"
                     /translation="MAVGQALSAPRPRSRHRVLKWVGGTLAALVTVIVLVLVFLDWNA
                     MRDPIGRYLSFRLHREVRILGDLNVRLFSWTPTAVVHDFVIAQPQWVTEGKSGQTGSF
                     VDVEQLTISLDLKQLFGWRIVLPTVDVEQPRLRFLRDASGRANWNFDEDKNAPPFRLP
                     PIRHFTIRDGHLDLTDARRNLVFAGTISSQENGGSLRENGGATERQFFNLVGKGQLNR
                     APFSAEVRGDPLLNIQPDRPYAFTGHVVAGATRIDAAGSLDKPFDLGAFQVKTTFAGP
                     DLADLYYLTGLTLPNTPAYKLSGNLTRDQETYRFSDFGGTVGESDLHGALTVDVSGDK
                     PYLKADLASQKLNFDDLGPLIGAQPGKSRGAIIAPAPKTAGTSIAITPAVSGAAPARG
                     ILPDTPLNIARLRQMNADVHYRAAMVVSRDFPLRGAETQLSLKDGVLKMTPVVFNFVH
                     GTLKGDVQIDARKDVPASDVDVRLTGLDLGQFLPSTSKTQALAGQAVARAKLHGTGNS
                     IHKAASTADGTVTFVVPHGEIRQTFAELLGINVASALGLLLTNDQSQVDVRCAVAGFS
                     SKNGIMTLDHFALDTPVVLASGEGTVDLKNERVNLSLSGHPKKPQLIRVRAPITINGP
                     LEHPAIGVDAGSAIVQGGLSAALAVVLSPLAAILPFVDPGLGKDADCGALLAQAKSRG
                     APVVQEQARR"
     gene            8973..10250
                     /locus_tag="VGM72_07130"
     CDS             8973..10250
                     /locus_tag="VGM72_07130"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4265073.1"
                     /translation="MNQDDLVFVKAGRRLIPFMMALYTVSFLDRVNIGFAALTMNTDL
                     GFTPEIYGWGAGIFFIGYFLFEVPSNVILTRVGARLWICRIMASWGLISAATAFVHSP
                     MSFYVLRFLLGLAEAGFAPGMIFYLSLWFPSTVRARYAAAYFLAIPLANVIGAPVSSL
                     ILGVDGVFGLHGWQWLFILEAVPALVLAAAVFMFLPNGPGDARWLSAREREVIASELA
                     ADRNSLDERVHGVWSALADFRVILLCAVYFGIVVGLYGIGYWLPQILQAMGYSNVVIG
                     VLVALPYSAAGLAQFLWGRHSDVKAERVWHVVLPSLTSAVAFGLSAALHAPSLVLIAL
                     AVAAIGICSALAPFWAMPPQFLSGRGAAAGIAAINAVGNLGGFIGPYAVGWAKESTGS
                     YAGGMAILAVSLLAAAVLVLAMGRSRRLALAAS"
     gene            10338..11276
                     /gene="fabD"
                     /locus_tag="VGM72_07135"
     CDS             10338..11276
                     /gene="fabD"
                     /locus_tag="VGM72_07135"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY4265074.1"
                     /translation="MRAFIFPGQGSQAVGMGKAIAEAFASAREAFEEVDDALSQSLTR
                     LMWEGPDSELVLTENAQPAIMAASMAIVRVLQSQGGLDLACHARVVAGHSLGEYSALC
                     AAGAFTLADTARLVRLRGQAMQAASPVGIGSMAALIGIDIEAAEAAVKEAANSGIVVI
                     ANDNAPGQIVISGEKAAVDKAGEIAKTKGAKRVIPLSVSTANHSPLLKPAGERMRDAL
                     AEIAIRPPLVPVVANVTASEVAEPDTIRRLLAEQVTARVRWRESVAGFAGLGVTTTVE
                     ASGNKVLTGMVKRIDKDLGTVTLDSPADIEAFAKTL"
     gene            11290..12030
                     /gene="fabG"
                     /locus_tag="VGM72_07140"
     CDS             11290..12030
                     /gene="fabG"
                     /locus_tag="VGM72_07140"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEY4265075.1"
                     /translation="MFDLTNKTALVTGASGGIGGAIAKALHAQGASVVLSGTRADALD
                     ALKAEMGGRAVTALCNLGNAAEVDALVKTAEAAIGAPIDILINNAGITRDNLLMRMKD
                     EEWDQVIAVNLTAAFRLSRAVLRGMMKKRWGRIVTITSVVGVTGNPGQGNYAAAKAGL
                     IGMSKALAAEVASRNITVNCVAPGFIATPMTDALTEQQKTAILTRIPAGKLGESEDVA
                     AAVVYLASDEAQYLTGQTLHVNGGMAMI"
     gene            12197..12433
                     /locus_tag="VGM72_07145"
     CDS             12197..12433
                     /locus_tag="VGM72_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY4265076.1"
                     /translation="MSDVAERVKKIVVEHLNVDGEKVTDNASFIEDLGADSLDTVELV
                     MAFEEEFGIEIPDDAAESIVTVGDAVKFIEKAQS"
     gene            12471..13742
                     /gene="fabF"
                     /locus_tag="VGM72_07150"
     CDS             12471..13742
                     /gene="fabF"
                     /locus_tag="VGM72_07150"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY4265077.1"
                     /translation="MSAMRRVVITGLGLVTPLGCGVETSWLRLLEGRSGAGPITRFKV
                     DDLPARIACDVPRGDGSDGSFNPDQWVDSKDQRRVDDFIIYAITASQQAVTDSGYEPK
                     TEEERDRAGVLIGSGIGGLDGIEQASILVHEKGPRRLSPFFIPGRLNNLASGYVSIRF
                     GYKGPNHSVVTACSTGAHAIGDAARMIALDDADVMLAGGTESAICRIGIAGFVACRAL
                     STGFNDTPEKASRPYDRDRDGFVMGAGAGIVMLEEYEHAKARGAKIYAEVKGYGMSGD
                     AHHVTAPAEDGNGGYRAMQMALKRAGLAAADIDYINAHGTSTPLGDEIELRAVERLFG
                     NSAGKLAMSSTKSSIGHLLGAAGAVEAIFATLAIRDQIAPPTLNLDNPSVQTEIDLVP
                     HKPRKMPISAALSNSFGFGGTNASLVLTAPE"
     gene            13770..14780
                     /gene="mltG"
                     /locus_tag="VGM72_07155"
     CDS             13770..14780
                     /gene="mltG"
                     /locus_tag="VGM72_07155"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY4265078.1"
                     /translation="MRVFVSLIVILALASASVAGAAWWEAGYFVQAGPSPSETTLVVK
                     PGSGLAAIAADLAKAGVVGNALLFRAAVIRRGRGAQLKAGEYAFPAHVSEAEAVALLI
                     AHRIVEHRITIAEGLTSDAAVALIDVDSVLSGPAPVVPEGSLLPETYLFERGTTRAEL
                     LARMHRAQEKVLTELWPKRKEGLPFTNQEDALKLASIVEKETAIASERPRIAAVFINR
                     LKNGMKLESDPTIIYGLTKGAALGHPLRQSELALPNPYSTYQIAGLPPTPIANPGRSA
                     IAAVLNPADTNELYFVADGSGGHAFASNIAEHQKNVAQWRRLQQTQNPPGPAAARPSR
                     LR"
     gene            14828..15718
                     /locus_tag="VGM72_07160"
     CDS             14828..15718
                     /locus_tag="VGM72_07160"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEY4265079.1"
                     /translation="MPLTSMTGFSDARGAHSGLRWRWEAKSVNGRGLDFRFRVPAGYE
                     GLEVGARTLAAERFKRGSFQVVLTVAAEDGGRGFRIDAAALADAVRIAKRVAEETGLP
                     PARVDGILALKGVVVQEESVALNDEARMARDAALLATLADAFDLLKSARATEGAKLEG
                     ILLLQIGEIERLTGDARRLAEQQPMALRERMMVQLKDLLAPGSSLSEERVAQEVALLA
                     ARADLREELDRLGAHCQEARALIRGIDPAGRKLDFLAQEFNREANTLCSKSSDIQLTH
                     VGLALKAVIDQFREQVQNVE"
     gene            15715..16347
                     /gene="gmk"
                     /locus_tag="VGM72_07165"
     CDS             15715..16347
                     /gene="gmk"
                     /locus_tag="VGM72_07165"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008034636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEY4265080.1"
                     /translation="MNEPIARRGLMLVISSPSGAGKTTLSRRLLAADPNIAMSVSVTT
                     RPKRLNEEHGKDYIFVDAEHFETMLREDAFLEHATVFANRYGTPRAPVEALLARGRDV
                     LFDIDWQGAQQLAQQAKDDLVRVFILPPSRAELERRLEGRAEDPPEIVARRMAEANNE
                     ISRWPEYDYVIVNDEIEKAQVQLEAIVAAERLNRQRQIGLVDFVRHLTQD"
     gene            16361..16939
                     /locus_tag="VGM72_07170"
     CDS             16361..16939
                     /locus_tag="VGM72_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265081.1"
                     /translation="MRVRHCLVALSVLTIAAVPFKFLSPAIALEGEAHDCAGASQDAV
                     TKLPMPLAKWGQISCTPAGHVLMSRDDWVWVMPDGSGMVFVPSQMGDKEPEEADNKSY
                     FTKIDVKPVKGDEFNQVYRTFHIGFDDSEVKPDAYQVDLATASGKTLRMYFFDYDSYA
                     WGMTCPDNKCAIDTRFMVLDKNHRPEPRQPAI"
     gene            complement(16982..>17498)
                     /locus_tag="VGM72_07175"
     CDS             complement(16982..>17498)
                     /locus_tag="VGM72_07175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HEY4265082.1"
                     /translation="IGVALPVPAVANLPFNVGTALLVKWLSADWPPWWQSLTLMFQKE
                     VAQRICAQPGDEAYGRLSVLAAWRTQTRILFDISRAAFVPPPNVTSSVVHIEPLPAPR
                     YGAELSALEKVTGAAFGQRRKMLRGSLKALTPDADALLEAASVPGTSRAEELDLAQFC
                     ALARAYCARRS"
BASE COUNT         3279 a         5290 c         5445 g         3484 t
ORIGIN      
        1 gatcaacgtt accgccaatt ggcgcctgat ccgggatttc gatccgctgc tgcgccaatc
       61 cgatgcgggg cgggtgctga tgatgagttc ggcggtggcg cggttgaacc gcccctattg
      121 ggggccctat gccatcacca aggccgccct cgaaatgctg accttcacct atgccgccga
      181 atgcaacggc accaaggtga tggcaaacgc ctacaatccc ggcccgacgc gcacgcgcat
      241 gcgcgccgaa gcgatgcccg gagaagatcc catgacgctg ccggcgcccg aggatctggt
      301 gccttccatt ctggaattgc tgtcgccggc ctctacgaaa aatggcgagc tggttgcgtt
      361 ccggcgctga ggctatttat cgctgtacgg gttcttgttt tcgcgcaggt tcaggcgcac
      421 cggtgtgccc ggcatctgaa acgcgccacg catcgcattc accagatagc gcagatagga
      481 atccggcagc gccttcagtt gattgccgaa cagcacgaag gtgggcgggc gcgccttggc
      541 ctgcgtcatg tagcggatgc ggatgcgccg cccatggacc gccggcggcg ggtggcgcga
      601 aagcgcctcc tcgagaaaac gattcactgc atgcgtggac acgcgcaggt tccagatgcg
      661 ctcggcttcg aacacggttg gcatcagccg ctccatgcct tcgccggtca gcgcggatac
      721 gcccacgatg ggtgcgcctg cgatctgcgg caggaagcgg tccacgcgct cgcgcaaatc
      781 cttgaacgcc gcacccctgt ccgccaccag atcccatttg ttgacggcga tgacgatcgc
      841 acgcccttcg cgcgcgatca ggtcggcaat cgcgaggtcc tgtttttcga gcgcctcgtt
      901 cgcgtccatc accacgatga cgcaatcggc aaaacgcacc gcgttgagcg tcgaattgac
      961 ggacagcttc tccagccgct cgtcgatgcg cgccttcttg cgcaagccgg ctgtgtcgtg
     1021 gagcaggata tcgcgctcct catgacgcca ttgcgccagc accgcatcgc gcgtgatccc
     1081 cgcctcgggg ccggtgagcg agcggtcttc gccgatgagc cggttgagga ggctcgactt
     1141 gccgacattg ggccgcccga caatggccag ccgaaggggc cggttttcca tttcggcttc
     1201 gggcgattcc gtggcttcct cggatggcgg aatttccggg aaatagaccg acagcgcatc
     1261 gcgcagttcc gcgagaccaa gcccatgttc ggcggagacc gcaatcggtt cgccgaaccc
     1321 gaggcgatag gcctcattgg cgcctgcttc ggcggcggtt ccctcgcatt tgttggccgc
     1381 gaggatcacg ggcttgccgc tgcgccgcag cgtctctgcg acgatctcat cgccagcggt
     1441 caatcctgcg cgcgcatcga ccacgaagag acagacatcg gcgtcacgaa tcgcggttcc
     1501 ggtctgcgtc gccatgcgcg ccaccaggct ctcgggcgcg cccttgtcga aacccgcggt
     1561 gtcgatgagc gcgaggtcca gtccggcgaa attacttgcc gcaaaggcgc ggtcgcgcgt
     1621 gacgccgggc gtgtcgtcga cgatggcgag acgcttgcca gccagccggt tgaacagcgt
     1681 cgattttccg acattcggcc ggcccacgat ggcgacggtc aggggcatct cagatagaac
     1741 ctcatgcccc tgaaatcaac gcagcgcgac gagctgcgcc tcattggtga gcacatacat
     1801 cataccgttg gcgacaatcg gcgggatgaa tgtgccatcg gggatggcgg tatcgcccag
     1861 cttttgaccc gtgaagggcg aaagcagcgt ggctttgccg tcggatgcga tcagcaccag
     1921 gaagttcgag accagcaacg gtccggtcca tacgatggga tcgcgctttc ccttcgggtc
     1981 ttcgaaaacc ggcaacgcgg ccgtccagcg tacgcgtcca tcgccgcgat tcatgcagac
     2041 gacctgaccg tcggtcgaca cgataaagat gtactctccg gccaccagcg gcgtctggat
     2101 gccggcgatg tttcgagtcc aggcgcggct gccggaccgg aaattgattg ccgccaatgt
     2161 gcccgaatgg ctgaccgcaa acaccatgtt gcggtcgatg accgggcgac cggcgatatc
     2221 gttgatgatg gtgagcgaag tggtgttccc ggaccgggtc agcgtgtccg tccaggacgg
     2281 cgtcccattc tgcgcccgca acgcgtagag ttcgcccgag ctgtaaggca cgacaaccgt
     2341 gtcgcctgac acgccgacgc tggcgctgga aaggatggca gcgctttcca cggtgccgtg
     2401 atggtcccaa aggcggtggc catcattcgc gtcgagcgcc tgcgtctcgt tttcctgggt
     2461 gatgacgtaa acccgaccgt ccaccaccac aggcgcggag tgtaccggaa ccaccgtgtt
     2521 gaccgaccac aattgttttc cggtcttggc gtccagcgcg gtgacattgc cgaagccggt
     2581 ggctgcgaag agtttgccgc tgtcaaacgc gattccgccg ccaaatcctt tggacggctt
     2641 gatggaattg ctggagccga aaatattgta ccacgaggag gtatcggtgc tttcaggtgc
     2701 gaggctcttg tcccagaccg gctttccggt ctgtgcatcg aaggcgaaaa cgtgggtttc
     2761 cgcatccaga acatagatga gcccaccggc cacgataggc ggggccgtca agcgcgaatc
     2821 gtcgtcggtg cccttgccgg ccgaaacgct ccacacttgt tcgagcggtc catcggccat
     2881 caaattgccg gtgacattgt cgggcgtacc tcccggctgc ggccaatccg cattcttctt
     2941 cggcggcggc aaatccatct tggtcgccgc cagcatggga tcggacgata gctgctgttc
     3001 ggacgcgatg atcgaaatgc gctcgccctg gatcttggtt ttcttggtcc tgtcgaacca
     3061 atccgccacc gtgttgtagg tgtcggcaca gccgccgagt atgccgcctg cgacaagcag
     3121 cagaacgaga gaggacgcga tacgcgactt catggttttg tcccttggcc ggatggcacg
     3181 ccactgccag gcgcaggcga agcaacaggt gcagcggcag cgggcaaaga tgaagccgcc
     3241 gccggcgcgc ccaagcttgg cagtgacggc ggcgttgggc cggatatggc cgcatccgga
     3301 ttggcgccga gatattcggc gatgcccgag gcgcgctgac gcagactggc gggcgcttca
     3361 gactcctgtg cgagtttttg gtattccgcg agcgcctggg aacgcgcccc attcttcaaa
     3421 tcggcatagg cgagaacttc agcggccgcg aagcgccagg gattgtccat cgccgtgagc
     3481 ggctggagca ggttaacgat atcggccttg gggctggcat ccgcgtcggt ccacgcaagc
     3541 ctcagccgtg ccgtgctcga aacgatggaa tcggaagatg ccgtgagttc acgcaacagc
     3601 gccacggcgg catcacgctt tccctgcgcg agataggccc cggcaagatg gaactttgca
     3661 agcgtcgcgt aacctgaagg cgcatccttc gccaaggctg cgaaatcggt ctccgccttc
     3721 ggcagattgc cggtttgcgc cgattggctg gcctcccgat accggtcgga tattttctgc
     3781 cgctgcgtca gatcatagcg ctgccaagcc tgatagcctg cgaccgcggc gaccagcaga
     3841 gccgcagcga tcatgatgta attgccgtat ttcttccaaa tctgttcgta tcgctcgcgg
     3901 cgtacgtctt cttcgacttc ctgaaagatg tcgctcacag attggaatcc tcaaaccttg
     3961 ctctcatgct tcgacagatt cgagccatgg aggcgggacg acacgcacat gcgcaagccc
     4021 tggatatgcc ggaatgctta gcgcgggtga gggcggctgg caaacctata tggctttcga
     4081 gcctttgcgc atcgcataga cattctcctt accggggaaa cggcggctgc gcacatcggc
     4141 cacaaaagag gttaccgccg cctcgatatc cggaccaaga tgggcatatt cccgcacgaa
     4201 acgcggcgga tgagggttca gccccagcat gtcttccatc accaggattt gaccgtcgca
     4261 ctccggtgag gcgccgatgc cgatggtggg aatggggatc gacttcgtga tcttggcagc
     4321 cagcggttca gcaatgccct cgatgacgac cgagaaggcg ccggcatcgg aaatggcttt
     4381 ggcatcggct tccagcgccg gccactcggc ttcgtcgcgc ccttgcgtgc ggaagccgcc
     4441 catcacttgc accatctgag gcgtgagccc gatatgcgcc atgacgggaa tcccgcgctc
     4501 ggtcaaataa tggatggttt cggccatgcg gacgccgcct tcgagcttca ccgcggtgca
     4561 gcccgtttcc ttcattgcac gcgaggcgtt gcgaaaggcg gtctcacggc tctcttcata
     4621 ggagccgaac ggcatgtcga tcgccacgag cgcccgcttg ctgccgcgca tcaccgcctg
     4681 gcaatgcagg atcatcagct cgagcgtcac gccaagcgtc gtttccatac cgtgcatcac
     4741 catgccgagc gagtcgccaa ccagcatcac atcgacatga ttgtcgagca gccgcgccgt
     4801 atgggcatgg tagcaggtga ggcaggcgat cggcgtgccg cccttgcggg cgcgtatctg
     4861 cggcacggtg atccggtcgg tttcagcttg agcggacatg gcacctccag tgcttgacgt
     4921 tagcacgcac cagccgccgg gccgagcata tccggcagtg gccgttctgg aacccgctgc
     4981 acacagaggc gaacacagga agcgtcgatt attccggcgt ttcttcctcg ccttcggcct
     5041 tcggacccgc gccttcctcg aacagggctt cagcgttggt ccgggcttcc tctgcctcgg
     5101 tctgctcggt cagcacatcc tcgccgcgcg cctggcgctc ggcttcatct tcggtgcggg
     5161 cgacattgat cgtgacctgt acccggacct ccgggtgaag caggacggaa atctcgtgca
     5221 gaccgatggt cttgatcgcc ttgtcgatcg gaacctgtgt gcgctgaatc gagaacccgc
     5281 ccgccgtggt ggcatcggcg atgtcgcgcg gactgacgga accatagagc tggccgcgat
     5341 cgccggcctg acgcaacagc acgaagctct tgccctgcag tttctcagcg attttctcgg
     5401 cttccttgcg ctgttccaaa ttgcgcgcct caagctgcac gcgctggctg tcgaaatagc
     5461 tgagatttgc cttcgtcgca cgaagcgctt tctttttggg caacaggaaa ttgcgggcga
     5521 agccgtcttt gaccttcacc acgtcgccga tctgacccag tttctcgacc cgttccaaaa
     5581 gcacaatctg catcggacgt gctcctattt cacaagatag gggatgagcg ccatgaagcg
     5641 cgcgcgcttg atggctttgg acagttcgcg ctgtttcttg gcgcagaccg ctgtaatgcg
     5701 tgaaggcacg atcttgccgc gttccgagac atagcgctgc aacagcttca catccttata
     5761 gtcgatctta ggggcgttgg agccggagaa cggacaggtc ttgcggcggc ggaagaaagg
     5821 ccggcgggcc agagggggtt gtgcgctcat accatggctc cttcggtctc ggcaccgtct
     5881 tcacgggggc ggcgttcgcc ttcacggccg cccgcgccgg cttcgcgttc acgcttgcgg
     5941 ctggaattgg actgttcgaa agcatcgacc ctgaccgtga tgaagcgcag gacgtcttca
     6001 ttgatcttca gctggcgttc gagttcggag atcgcgttgg ccggcgcatt caggttcagg
     6061 agcacataat gccccttgcg gtttttcttg atgcggtagg caagtgtgcg caggccccaa
     6121 tattcctgct tctcgacctt gccgccttcg gtctcgatga tcgttttcaa atgggtggcc
     6181 agtgcgtcca ccgcctgcgc gctgatatcc tggcgcgcga tcagcatgtg ctcatagaga
     6241 gccatgtgca ttccttcctg tagaggtgcg gcatgccgag cgagaaggtt tcgcatcgca
     6301 tgtcggttcg tgaagcgccg gacatccggc ataaccttct gaagttagag ggccttcgcg
     6361 accgcacccg gtctcaaggg cgcgggatat agccgaactg gcgcgcctca gcaatggcgt
     6421 atcccgcgcg gagcgccggc ctagtccgaa ggcgcggcta agccttcaac tttcctgaat
     6481 aattgaactc tttcgcgggt ttgaggcgtt taccaagggt agtcatcgac aaaggccaaa
     6541 gattgccctg gtccaatttc aagacaaaat tgcaggccgc ttttggccgc aatacgtcta
     6601 tggcggtggg acaggccctg tctgctccac ggccaaggtc ccgtcaccgt gtcttaaaat
     6661 gggtgggcgg cactttggcc gcactggtga cggtgattgt tctggtgctc gtgttcctcg
     6721 actggaatgc gatgcgcgat ccaatcgggc gttatctttc gttccgcctc catcgcgagg
     6781 tgcggattct gggcgatctg aacgtcaggc tgttctcatg gacccccacg gccgtggtcc
     6841 atgatttcgt catcgcgcag ccgcaatggg tcacagaagg caagtctggc cagaccggat
     6901 ccttcgtgga tgttgagcag ctcacgattt cgctcgatct gaagcaactg ttcggctggc
     6961 gcatcgtgct gccgaccgtg gatgtggagc agccacgcct gcgattcctg cgcgatgctt
     7021 cgggccgcgc gaattggaat ttcgacgaag acaagaacgc gcctccgttc cggctgcccc
     7081 ctattcgcca tttcacgatc cgtgacggac atctcgatct taccgatgcc aggagaaacc
     7141 ttgttttcgc gggcacaatc tcgtcgcagg agaacggtgg ctcattgcgg gagaacggtg
     7201 gcgcaacgga gcggcaattc tttaacctgg ttggcaaggg acaactcaat cgcgcgccat
     7261 tttccgccga agtcagaggc gatccgctgt tgaacattca gccggatcga ccctatgcgt
     7321 tcaccggcca tgtcgtggcg ggggcgacaa ggatcgatgc cgccggctcg ctcgataagc
     7381 ccttcgatct cggcgcattc caggtcaaga ccactttcgc cggacccgat ctcgcggacc
     7441 tttattacct gacgggtctg acgctgccca acacgcctgc gtataaactg tccggtaatc
     7501 tgactcgcga tcaggagaca taccgcttca gcgatttcgg cggtaccgtc ggcgaaagcg
     7561 acctgcatgg cgcgcttacg gtggatgtct cgggcgacaa accttatctg aaagccgatc
     7621 ttgcatcgca gaaactgaat ttcgacgatc ttggcccgct gattggtgca caaccgggaa
     7681 aatcgagggg tgcgatcatc gctccggcgc cgaagacggc aggaacctcc atcgcgatta
     7741 caccggcggt tagcggcgcg gcgcccgcgc gcggcatact tccggacacg cctttgaaca
     7801 tagcccgcct gcgtcagatg aatgccgatg tgcattatcg cgcggcgatg gtggtttcgc
     7861 gcgatttccc attgcgcggg gccgaaacac aattgtcatt gaaggacggt gtgctgaaaa
     7921 tgacaccggt tgtcttcaat tttgtgcatg gcacgctgaa aggcgacgtg cagatcgatg
     7981 cgcgcaagga tgtgcccgcc agcgatgtcg atgtccgcct cacgggactc gatctcggtc
     8041 aatttctgcc cagcacgagt aagacgcagg cattggctgg acaggcggtc gctcgcgcga
     8101 agttgcatgg caccggcaat tcgattcaca aggcggcgtc cacggcggac gggaccgtca
     8161 ccttcgttgt gccgcatggc gagatacgcc agacattcgc cgaacttcta gggatcaatg
     8221 tcgcaagcgc actcggcctg ttgctgacca acgatcagag tcaggtcgat gtccgttgcg
     8281 cggtggcggg gttctcttcg aaaaatggca ttatgacgct ggatcatttc gcgctcgata
     8341 cgcccgttgt cttggcgagc ggcgagggta cggtcgatct caagaatgaa agggtcaatc
     8401 tttcgctcag cggccacccg aaaaagccgc agctcattcg tgttcgcgcc ccgataacca
     8461 tcaacgggcc gttggagcat cctgccatcg gtgtcgatgc cggcagcgca atagtccagg
     8521 gcggattgtc cgccgctctt gccgtcgtcc tgtcgccgct tgcggctatt ctgccgttcg
     8581 tggatccggg actgggcaag gatgccgatt gcggtgcatt gcttgcacag gcaaaatcgc
     8641 gaggcgcgcc cgtcgtgcag gaacaagcgc ggcgctagct cgtctcgaag aatcaccgaa
     8701 acgaaaaccc accccaaaat gaaaaaccaa agggcactgc accagcagcg ccctttggat
     8761 ttgcggtcct ccagctgttg gcatcaggac cgaccggccc ggcgggcggg cccgtctttg
     8821 cgacctaatc ctcataaagg aaggccgtgc atagattccg cctttacagc gttcgcgtca
     8881 atcgcgatgc gaaaaatgtg atcgggcttg ttgcagcggc agtaatgccc gaaaaccggc
     8941 cgccggattc accggtccgg ggcaggcagc gcatgaatca ggatgacctt gttttcgtca
     9001 aggcggggcg gcgtctcatt ccattcatga tggcgctcta tacggtcagt tttctcgatc
     9061 gtgtgaacat cggtttcgca gcgctgacca tgaacacgga ccttggcttc acgccggaga
     9121 tctatggctg gggcgccggt attttcttca tcggttattt cctcttcgaa gtgccgagca
     9181 atgtgatcct gacccgtgtc ggcgcgcgcc tttggatctg ccgcatcatg gcgagttggg
     9241 ggctgatctc ggcagcgacc gcattcgtgc atagcccaat gagtttctac gtgctgcggt
     9301 ttctattggg cctcgcagaa gccggttttg ccccgggcat gatcttctat ctgtcgctct
     9361 ggtttccctc cacggtgcgg gcgcgctatg ccgccgccta ttttctcgcc attccgctgg
     9421 cgaacgtgat tggcgcgccg gtctcgagtc tcatcctggg cgttgacgga gttttcggtc
     9481 tgcatggctg gcaatggctg ttcatcctcg aagccgtccc cgcgcttgtg ctagcagccg
     9541 ccgttttcat gtttctgcct aatggtccag gcgatgcacg ctggttgtcg gcgcgcgagc
     9601 gtgaggttat cgcctcggaa cttgcggccg accgcaattc cctggacgaa cgcgttcatg
     9661 gcgtttggag tgccttggcg gattttcgcg tgatcctcct gtgcgcggtc tatttcggaa
     9721 tcgtcgtcgg tctctatggg atcggttatt ggctgccgca gatcctccag gcgatgggat
     9781 attcgaatgt cgtgatcggc gtgcttgtgg cgttgcccta ttctgcggcc ggcctcgcgc
     9841 aattcctctg gggccggcat agcgacgtga aagccgagcg cgtatggcac gtggttcttc
     9901 catcgttgac gagcgccgtc gctttcggat tgtccgcagc gctgcatgcc ccgtcgctcg
     9961 tgctgatcgc gctggctgtc gccgccatcg gaatctgctc ggcactcgcg ccattttggg
    10021 caatgccgcc gcaattcctc tccgggcggg gcgccgccgc aggcatcgcc gccatcaacg
    10081 cagtcggcaa tcttggcggg ttcatcgggc cgtatgctgt tgggtgggca aaggaatcca
    10141 cgggttctta tgccggcggc atggcaatcc ttgcggtctc gctgcttgcg gcagccgttt
    10201 tggttctggc gatgggacgg tcgcgccgtc ttgcgcttgc ggcgtcctga cgcaaattat
    10261 tttgcgggcc taccgctctt cgaagcatct tgcgctggac gaagcctcga tttaaggggc
    10321 ttcaaacgga ggccatcgtg agagcattca tttttccagg ccaaggcagt caagctgtcg
    10381 gtatgggcaa agccatagcc gaagccttcg cctcggcgcg cgaagcgttc gaagaggtgg
    10441 atgatgccct gtcccaaagc ttgacccggc tgatgtggga aggccccgat tccgaattgg
    10501 tcctgacgga gaatgctcag cccgcgatca tggcggcctc gatggcgatc gtccgcgtgc
    10561 tgcaaagcca agggggactt gacctcgcgt gccacgcccg agtggtggcc ggccattctc
    10621 tgggggaata ttcggcgctc tgcgcggcgg gcgctttcac gctcgccgat accgcgcgtc
    10681 tcgtgcgcct gcgcggacaa gcgatgcagg cagcctcccc ggtgggtatc gggagcatgg
    10741 cggcgctcat cggtatcgac atcgaagcgg ccgaagcggc cgtcaaggaa gccgccaatt
    10801 cgggcatcgt ggtcatcgcc aatgataatg cgccgggcca gatcgtgatt tccggcgaga
    10861 aggccgccgt ggacaaagcc ggcgagattg ccaagaccaa gggcgcaaag cgcgtgatcc
    10921 cgctttccgt cagcacggcc aatcattcgc ccctgttgaa acccgcaggt gagcggatgc
    10981 gcgatgcgct ggcggaaatc gccatccgtc cgcccctcgt tcccgtggtg gcgaatgtga
    11041 ccgcttcgga agtggccgaa cccgacacga tcaggcggct gctcgccgaa caggtcaccg
    11101 cgcgcgtacg ctggcgcgaa agtgtggccg ggttcgctgg cctcggagtt acaacaaccg
    11161 tggaagccag tggaaataag gtgctgaccg gcatggtcaa acggatcgac aaggaccttg
    11221 gcaccgtgac actcgattcg cccgccgaca ttgaagcttt cgcgaaaacg ctctgaggtt
    11281 ttttcgatta tgttcgatct caccaacaag accgcgcttg tgaccggcgc ttcgggcggc
    11341 atcggcgggg ccatcgccaa agcgttgcac gcccaaggcg cgagcgtggt tctgtcgggc
    11401 acacgcgccg atgcgctcga cgcgctgaaa gccgaaatgg gcggacgggc cgtcaccgct
    11461 ctctgcaatc tcggaaatgc ggccgaggtg gacgcgttgg tgaagaccgc cgaagccgcc
    11521 atcggcgccc ctatcgacat cctcatcaac aatgcgggca tcacacgcga caatctcctt
    11581 atgcgcatga aagacgaaga gtgggatcag gtcatcgccg tcaacctcac cgcagcgttt
    11641 cggctgtcgc gtgcggtgct ccgcggcatg atgaagaagc gctgggggcg gatcgttacg
    11701 atcacctctg tggtgggcgt tacgggcaat ccggggcagg gaaattacgc cgcggcgaag
    11761 gccggactga tcgggatgag caaggcgctg gcggccgaag ttgccagtcg caacatcacg
    11821 gtcaattgcg tagcccccgg ttttatcgca acaccgatga cggatgcgct caccgaacag
    11881 caaaaaaccg cgattctcac gcgcattccg gctggaaaac ttggcgaatc ggaagacgtc
    11941 gcagcagccg tcgtctatct tgcaagtgac gaagcgcagt acctcacggg ccagaccttg
    12001 catgtcaatg gcggcatggc aatgatctaa ggcattggtt aaaaagactc tttcgaaagt
    12061 ttggcgggaa ccgaaagttt gtgataccaa gcgcgaccct ttcaaccatt gctgtgacag
    12121 tgcccgggtc gcgtgcccaa ctcacaaagc gtggtaagag aaaaacaaca acggcaattc
    12181 aagaggcaaa gaacatatga gcgacgtcgc tgagcgcgtg aaaaaaatcg tggtcgaaca
    12241 tctgaacgtc gacggcgaaa aggtcacgga caatgccagc ttcattgaag atctcggcgc
    12301 ggacagcctc gacaccgttg agctggtcat ggcgttcgag gaggaattcg gcatcgaaat
    12361 tccggatgat gctgccgaat ccatcgtcac tgtcggggat gcggtgaagt tcatcgagaa
    12421 ggcccagagc tgatcgctaa aaactggcaa cagccggggc cctcgcgcca atgagcgcaa
    12481 tgcgtcgtgt cgtcatcaca gggttggggc tcgtaacccc gctgggctgc ggggtcgaga
    12541 cttcgtggtt gcgcctgctc gagggcaggt ccggtgcggg ccccatcacg cgtttcaagg
    12601 tggacgacct gccggcgcga atcgcctgcg acgttccgcg cggtgacggc tccgacggca
    12661 gcttcaatcc ggaccaatgg gtggattcca aggatcagcg ccgggtcgat gatttcatca
    12721 tctacgcgat caccgcctct cagcaggcag tgaccgattc cggctacgag cccaagaccg
    12781 aggaggagcg cgaccgggcc ggagttctga tcggttcggg tatcggtggc ctggacggca
    12841 tcgagcaggc gtccattctc gttcatgaaa agggaccccg gcggctgtcg ccctttttca
    12901 ttccagggcg cctcaataat ctcgcatcgg ggtatgtctc catccggttc ggctacaagg
    12961 gacccaatca ttccgtcgtg acggcctgct cgacgggggc gcacgcgatc ggggatgcgg
    13021 cgcgcatgat cgcgctggac gatgccgatg tgatgctggc cggcggcacc gaatcggcga
    13081 tctgccgtat cgggatcgcg ggtttcgtag cctgccgggc actgtccacc ggcttcaatg
    13141 atacgccgga aaaagcctcg cgcccctatg acagggatcg cgacggcttc gtgatgggtg
    13201 cgggtgcagg catcgtgatg ctcgaggaat atgagcatgc caaggcccgt ggcgcgaaga
    13261 tctatgccga agtgaagggt tatgggatgt cgggcgatgc ccatcacgtc accgcgcccg
    13321 ccgaggatgg caacggcggc taccgcgcca tgcaaatggc gttgaagcgc gcggggctcg
    13381 ccgcggccga tatcgattac atcaacgcgc acggcacctc cacaccgctc ggcgatgaga
    13441 tcgaacttcg cgcggtcgag cgcctgttcg gcaattcggc gggcaagctt gcgatgtcct
    13501 cgaccaaatc ttcgatcggc catttgctgg gcgcggccgg cgccgtcgag gcgatcttcg
    13561 ccacgctcgc gatccgcgat caaatcgccc cgccgaccct caacctggac aacccgtcgg
    13621 tccaaaccga aatcgacctc gtgccgcata aaccgaggaa aatgccgatc tcggctgcac
    13681 tctcgaattc ctttggtttc gggggcacca atgcatcgtt ggtgctgacc gcgccggaat
    13741 agagcgaacg cccgaacatc gaggccgtca tgcgggtttt tgtctccctg attgtcattc
    13801 tcgccttggc gtcggcttcg gtcgcgggag ccgcctggtg ggaggccggt tatttcgtgc
    13861 aggccggacc ctcgccgtcg gaaacgacgc tggtggtgaa acccggaagc ggtcttgccg
    13921 cgatcgccgc ggacctggcc aaggcggggg ttgtcggcaa cgcactgctg tttcgcgccg
    13981 ctgtcattcg gcggggccgc ggggcgcaat tgaaagccgg cgaatatgcc tttcccgccc
    14041 atgtcagcga ggctgaggcc gtggccctgc tcatcgcgca tagaatcgtc gaacatcgca
    14101 tcaccattgc cgaaggtctc acctccgatg ccgcggtcgc actcatcgat gtcgattccg
    14161 ttctgagcgg accagcgccg gtggtccccg aaggcagcct tctgccggag acctatctgt
    14221 tcgagcgcgg gaccacacgc gccgaattgc tcgcgcgcat gcatcgggcg caggagaagg
    14281 tgctcaccga actttggccc aagcgcaagg agggcttgcc tttcacaaac caggaggacg
    14341 cgctgaagct cgcctccatc gtcgagaagg aaactgcgat cgcatcggaa cggccgcgca
    14401 tcgccgcggt cttcatcaac cgtctgaaga acggtatgaa gctcgaaagc gatcccacga
    14461 tcatctacgg cctgacgaag ggcgcagcgc tcggccatcc cttgcgccag agcgaacttg
    14521 ccctgcccaa tccgtacagc acctatcaga tcgccggcct gcccccgacg cccattgcca
    14581 atccgggcag gagcgcgatt gctgccgtcc tgaaccccgc cgacacgaac gagttgtatt
    14641 tcgtcgcgga cggcagtggc gggcatgcct ttgcaagcaa tattgccgag catcagaaga
    14701 atgtcgcgca atggcgccgg ctgcagcaga cgcaaaaccc tcccggtcct gcggcggcgc
    14761 ggccgtcccg cttgcgctag cgtttcgttc gtgccgagat cgcttttttc gattccagga
    14821 gacggatttg ccccttacca gcatgaccgg attctccgac gcgcgcggcg cccatagcgg
    14881 gctgcgctgg cgttgggagg ccaagagcgt caacggccgg ggattggact tcagatttcg
    14941 cgtgcctgcg ggctacgaag gtctggaagt cggcgcacgc acgctcgcgg cagagcgttt
    15001 caaacgcggt tcttttcagg tcgtgctcac cgtcgcggcc gaggatggcg gccgcggttt
    15061 ccgcatcgac gcggcggcgc tggccgatgc ggtccgcatc gccaagcggg tggcggagga
    15121 aaccggcttg ccgcccgcgc gcgtcgatgg aattctcgct ctcaaaggcg tggtggtgca
    15181 ggaagaaagt gttgccctga acgatgaggc gcggatggcg cgcgacgcgg ccttgctggc
    15241 gacgctcgcc gatgcgttcg atctgctcaa atcggcgcgg gccaccgaag gcgcgaagct
    15301 ggaagggatt cttttgctgc agattggcga gatcgaacgg ctgacgggcg atgcgcgcag
    15361 gcttgcggaa cagcagccca tggccttgcg ggagcgcatg atggtgcagc tgaaggatct
    15421 gctcgcaccc ggctccagcc tttcggagga acgcgtcgcc caggaagtcg cgctgttggc
    15481 ggcccgcgcc gatctgcgcg aagaactgga ccggcttgga gcccattgcc aggaagcgcg
    15541 agcgcttatc aggggaatcg atcctgccgg acgcaagctc gatttcctgg cgcaggaatt
    15601 caatcgcgag gcgaatactc tctgttcgaa atcctcagac attcagctca cacatgtcgg
    15661 gctcgcgctg aaagccgtca tcgatcaatt ccgcgaacag gtgcaaaacg tcgaatgaat
    15721 gagcccatcg cccggcgcgg tctgatgctg gtgatttcct cgccgtccgg cgcaggcaag
    15781 acgacgttgt cgcgccgctt gctggcggcc gaccccaata ttgcgatgtc ggtctcggtg
    15841 accacgcgcc ccaagcgcct gaacgaggag catggcaaag attatatctt cgtcgatgcg
    15901 gaacatttcg agacgatgct gcgcgaggac gcctttctgg agcatgcgac ggtcttcgca
    15961 aatcgctacg gcacacccag agcccccgtt gaagccttgc tggcgcgggg gcgcgatgtt
    16021 ctgttcgaca tcgactggca aggggcccag caattggccc aacaggcaaa agacgatctg
    16081 gtgcgggtct ttatcctgcc gccgtcgcgc gccgaattgg agcgccgtct cgagggccgc
    16141 gcggaagatc ctcccgaaat cgtggcccgc aggatggcgg aggccaacaa tgaaatcagc
    16201 cgctggccgg aatatgatta tgttatcgta aacgacgaaa tcgagaaagc gcaggtacag
    16261 cttgaagcca ttgtcgcggc cgagcgttta aaccggcaga gacaaatcgg tctggtggac
    16321 tttgtcaggc atctgaccca ggactaaagg agatcgcgcg atgcgggttc gccattgttt
    16381 ggttgcgctc agtgtgctga cgattgccgc cgttccgttc aagttcctat ctcccgccat
    16441 tgccttggaa ggcgaagccc atgattgtgc cggtgcatcg caagatgcgg tgaccaagct
    16501 tcccatgccg ctcgcgaaat ggggtcagat ttcctgcacg cccgccggac atgtgctgat
    16561 gagcagagac gattgggttt gggtcatgcc ggatggatcg gggatggtct tcgtgccttc
    16621 ccagatggga gacaaggaac ccgaagaggc tgataacaaa tcctatttta ccaagatcga
    16681 cgtaaagccg gtcaagggcg acgaattcaa ccaagtttac cggaccttcc acatcggttt
    16741 cgacgacagc gaagtgaagc ccgatgccta tcaggtcgat ctcgcgacgg cgtccggaaa
    16801 gacgctgcgg atgtatttct tcgactatga cagctatgcc tggggtatga cctgtccgga
    16861 caacaaatgc gccatcgaca cgcgcttcat ggtgctggac aagaatcatc gtccagagcc
    16921 gcgccaaccc gcgatctgat gtttcggctg ccgatgacga tacggtagcc gcagtgcata
    16981 ttcatgaccg ccgggcgcaa taggctcgcg ccaacgcaca gaattgagcg agatcgagtt
    17041 cttcggcacg cgacgtgcct ggcacgcttg cggcttcaag caatgcatcg gcatccgggg
    17101 tgagcgcttt gagacttccc cgcagcatct ttcgcctttg gccgaatgcg gccccggtta
    17161 ctttttcgag cgccgaaagt tctgccccgt atcgtggtgc gggcagaggc tcgatgtgca
    17221 caaccgagga ggtcacattg ggcggcggca caaaagcggc gcgcgaaata tcgaacagga
    17281 tgcgcgtctg tgtgcgccat gcggcgagca ccgaaagccg gccatacgcc tcgtcacccg
    17341 gttgagcgca aatccgttgg gccacctcct tttggaacat cagggtcaga ctttgccacc
    17401 agggcggcca atcggcactc agccatttga cgagcagcgc tgtccccaca ttgaacggca
    17461 gattggcgac ggccggcacc ggcagagcca cgccaatg
//
LOCUS       DASYEG010000156         7806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4830921, whole genome shotgun sequence.
ACCESSION   DASYEG010000156 DASYEG010000000
VERSION     DASYEG010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4830921"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VGM72_07180"
     CDS             <1..703
                     /locus_tag="VGM72_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="This protein performs the mismatch recognition step
                     during the DNA repair process; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY4265083.1"
                     /translation="MGAFVPAASAHIGIVDKLFSRVGAADDLARGRSTFMVEMVETAA
                     ILNQASERSFVILDEIGRGTATFDGLAIAWAVVEHLNEVNKSRALFATHYHELVTLCE
                     RLTDVACATMRVKEWKDNVVFLHEVAPGAADRSYGIQVAKLAGLPGAVTARATEVLRA
                     LEEGREGHKPLARIDDLPLFAEKLREPKQATPKSSALEDALRAADPDSLTPKAALELV
                     YTLKRLAADPSTGSG"
     gene            795..1292
                     /locus_tag="VGM72_07185"
     CDS             795..1292
                     /locus_tag="VGM72_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY4265084.1"
                     /translation="MSDVSGLQTLPPNRRKFLVVVDQTPECKVALRFAARRAQHTGGI
                     VTLLYIVAPADFQQWAGVERRMREEAREAAESALHEAAKTVNDIVGTMPELVIREGRP
                     AEEIVALLKEDRALAILVLATGTSKEGPGPLVSLISAPAATAYPIPVTIVPGTLTDEE
                     VDALA"
     gene            1338..3101
                     /locus_tag="VGM72_07190"
     CDS             1338..3101
                     /locus_tag="VGM72_07190"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY4265085.1"
                     /translation="MRLDRPEGTPDGTIGWGSDIAAAMLRRFNIPFISLNPGASYRGF
                     HDSLVNHLGNENPGILLCLHEDHSVAVAHGYARVTGEPMACVLHANVGLLHGMMSMFN
                     AWCDRAPMLVLGATGPLASEKRRPWIDWIHTMRDQASLVRSFVKWDDQPSSPDALAES
                     LARAHLLTRSHPTAPVYVCLDAGLQESKLDREPDWPDLARLQPPAPPRPAKDAVEKAA
                     ALLRAAARPVILAGRGSRSDAAWKARLLLAERLGAVVMSDLKSGAMFPTGHPAHVVAP
                     FNALPKLAREILCEADAILSLDWIDLGGALKQASAAGQVTAGIVSASLDQTLHNGAGM
                     EYQALAPADVFMATTADALVEELVAVLGAGNKAPWKIRAPTKRKEASGAAVTLDGIAS
                     ALRSAFNDPEKVTFSLLNRGWPSDIWPFKSALAYLGKDGGGGLGSGPGLSVGAAVALE
                     RMGRYAVSVLGDGDFCMGATAIWTAVHHRIPMLILIDNNRSYFNDELHQQNVARARGR
                     DVRNRWIGLRMEDPMPDIATLARGQGAVGIGPVTRAADVAAALEEGVGVLKQGGVCVI
                     DFHVDPGEDRTSAASVGQRKT"
     gene            3208..3486
                     /locus_tag="VGM72_07195"
     CDS             3208..3486
                     /locus_tag="VGM72_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265086.1"
                     /translation="MLLNIVNGYVCHNCTDVENAKKGVDPAHPKKDSAPQSPTKTGIA
                     QSNPAVILSGALAKTGAAKPAGSGLTNQAPNAPPLAIPETGDSVNLSI"
     gene            complement(3509..3832)
                     /locus_tag="VGM72_07200"
     CDS             complement(3509..3832)
                     /locus_tag="VGM72_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265087.1"
                     /translation="MTQTGIPSHFEQRVGLKFQLVASLQSVRWEPFVTYIAALMEKKI
                     PVFLSVPGPAGQDSRVVFLNDGMAAAVATRNSRQIVQELMRALEMRHRRKELTGSDNS
                     ASDLS"
     gene            4687..5253
                     /locus_tag="VGM72_07205"
     CDS             4687..5253
                     /locus_tag="VGM72_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017532211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEY4265088.1"
                     /translation="MFIQTETTPNPATLKFLPGREVMASGTVDFPDAEAGKRSPLASR
                     LFAIAEVERVFFGSDFVTVTKRESDAIDWRHLKPMILGAIMEHFTRGLPLFDSEGEAE
                     AEPIYEGEEGEIVAQIKELIETRVRPAVASDGGDIIFKGFDGGSGTVFLHLQGSCSGC
                     PSSTITLKNGIENMLRHYVPEVSAVEAV"
     gene            5332..6696
                     /locus_tag="VGM72_07210"
     CDS             5332..6696
                     /locus_tag="VGM72_07210"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY4265089.1"
                     /translation="MKFYSQVAQDRFLLENFFRGRRGGVFLDIGAYDGETLSNTAFFE
                     RSMGWTGLCVEPLPSAFAKLTARRKALCECVCVADFEGEADFLESDTASPDEMMLSGM
                     PAHFDPRHVARLDECATTRVTRRVPVTKLSTLLTKHRLFDIDYCSIDTEGAELAILSD
                     FDPERFRIKLFTIENNWDDPRIPDLMAQKGYELFAKLEWDNVYRRRDVKPLPRTSVIC
                     AVWHGDPNRHALLRGHAENLARQSVPVEPIYVFDNGDAPPPWLEARAISVKEPLTIYQ
                     AWNVALSLVATPLAMNLNLDDRLAPDAIGMLEQELLKNRAVAAGGDWKVCYAQAETDD
                     VAPCFPVSGVPFLPAWPPARGSLTRLGSGTGERGTYGPATIWRMDAHVGAPRYPWRLR
                     DGSLLRASADLAWWQMLQSNPNFKILAIPMVIGNYHSHPEGQAEFREADERAAMADPG
                     VSLT"
     gene            6745..7443
                     /locus_tag="VGM72_07215"
     CDS             6745..7443
                     /locus_tag="VGM72_07215"
                     /inference="COORDINATES: protein motif:HMM:NF024931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEY4265090.1"
                     /translation="MTVAHVYTVAFEGIAAREVDVQVHIGEGGAGVFTVVGLGDKAIG
                     ESRERVRAALAAVGLALPWKRITVNLAPADLPKEGSHYDLPIALALLAAMGVLPVSEM
                     AGYTAMGELSLDGQITAVAGVLPAAIAAAEAERGLICPAACGAEAAWAGGVEIIAAPS
                     LIALVNHIKGAQVLGVPEARLAESAVSIPDLKDVKGQETAKRALEIAAAGGHNMLTLW
                     SSYPASCFSNQSFS"
     gene            7440..>7806
                     /locus_tag="VGM72_07220"
     CDS             7440..>7806
                     /locus_tag="VGM72_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265091.1"
                     /translation="MSRDPAHILLSTNNSEVPQLPKRKTGRFITEFIAVFFWIYALTK
                     LFIFDLDIWIINLVSPQFASILNYKLPIILVGISIAIVTTRSARLGLSALYIAFYPLV
                     ILSWKIPRFVWHQKSWLLAF"
BASE COUNT         1452 a         2404 c         2457 g         1493 t
ORIGIN      
        1 gatgggcgcg ttcgtgcccg ccgcaagcgc gcacatcgga atcgtggaca agcttttctc
       61 ccgcgttggc gcggcagatg atctggcgcg tgggcggtcc acgttcatgg tcgagatggt
      121 cgagaccgcg gcgatcctca atcaggcgag cgagcgctct ttcgttattc ttgatgaaat
      181 cggccgcggc acggcgacgt tcgatggttt ggcgattgca tgggcggtgg tcgagcatct
      241 gaacgaggtc aataaatccc gcgcgctgtt cgcgacgcat taccacgagc tggtcacgct
      301 ctgcgaacgc ttgacggatg tcgcctgcgc cacgatgcgg gtgaaggaat ggaaggacaa
      361 tgtcgtcttc ctgcatgaag tcgcgccggg cgcggcggac cgttcctatg gcatccaggt
      421 cgcgaaactg gcgggattgc cgggcgcggt gacggcgcgc gccaccgaag tgctgcgcgc
      481 gctggaagag ggacgcgaag gccacaagcc gctcgcgcgc atcgacgatc tgccgctttt
      541 cgcggaaaaa ttgcgcgagc cgaaacaagc cacgccgaaa tcgagcgcgc tggaagatgc
      601 gctacgcgcc gccgatcccg acagcctcac gccgaaggcc gcgttggaat tggtctacac
      661 gctgaaacgc ctcgccgctg acccttcaac aggctcaggg tgaaggaccg tatcttttcc
      721 tcatgctgag cttgtcgaag catgagcgcg cgacaccttg cgagccggac ggcaagcagg
      781 cacgatagag tccgatgagc gatgtttccg gcctgcaaac gcttccgccc aatcggcgca
      841 aattcctcgt cgtcgtcgac cagacgcccg aatgcaaagt cgcgctgcgt ttcgccgcgc
      901 gccgcgccca gcacaccggc ggcatcgtca cgctgctcta catcgtggcg ccggcggact
      961 tccagcaatg ggcgggtgtc gagcgcagga tgcgcgagga ggcgcgcgag gccgccgaga
     1021 gcgcgttgca cgaagcggcc aagaccgtga acgacattgt cggcaccatg cccgagcttg
     1081 tcatccgcga ggggcgcccg gccgaagaga tcgtggcgct tctgaaagaa gaccgcgcgc
     1141 tcgccatcct ggtgctcgcg accggcacct cgaaagaagg cccgggcccg ctggtctcgc
     1201 tcatctcggc gcccgcggcc acggcctatc ccatcccggt caccatcgtg ccgggcacgc
     1261 tgaccgatga agaggtcgac gcgctggcgt gaagcgccac gccgtgctca cattgcccaa
     1321 cacgattggg gatgcgcatg cggctggaca gacctgaggg aacgccggac ggcacaatcg
     1381 gctggggcag cgacatcgcc gcggcgatgc tgcgccgctt caacatcccg ttcatcagcc
     1441 tcaatccagg cgcctcctat cgcggctttc acgattcgct ggtcaatcat ctgggcaacg
     1501 agaatccggg catcctgctc tgcctgcacg aggatcattc ggtcgccgtc gcccatggct
     1561 atgcgcgggt gacgggcgaa cccatggcct gcgtgctgca cgccaatgtc ggtctgctgc
     1621 atggcatgat gagcatgttc aacgcctggt gcgaccgcgc ccccatgctg gttttgggcg
     1681 ccaccggtcc gctcgcgtct gaaaaacgac gcccctggat cgactggatc cacaccatgc
     1741 gcgatcaggc atccctggtg cgctccttcg tgaaatggga cgatcagccc tcctcgcccg
     1801 atgcgctcgc ggaatcgctc gcccgcgccc atctcctgac gcgttcgcac cccacagcac
     1861 ccgtctatgt ctgcctggat gcgggcttgc aggaatccaa actcgatcgc gaaccggact
     1921 ggcccgatct cgcgcggctg caaccgcccg caccgccgcg cccagcgaaa gacgcagtgg
     1981 agaaagccgc cgccttgctc cgtgccgccg cgcgccctgt gatcctcgcg gggcgagggt
     2041 cacgcagcga tgcggcgtgg aaggcgcgtc tgcttttggc cgaacgtctc ggcgcggtgg
     2101 tgatgagcga tctgaaatcg ggcgcgatgt ttcccaccgg ccatcctgcc catgtcgtgg
     2161 cgccgttcaa cgcactgccc aaacttgcgc gcgaaatcct gtgcgaagcc gacgcgatcc
     2221 tgtcgctcga ttggattgat ctcggcggcg cgctgaaaca ggcgagtgcg gcgggccaag
     2281 tgaccgctgg aatcgtcagc gcctcgctcg atcaaactct gcacaatggc gcgggcatgg
     2341 aatatcaggc gctcgcgccc gccgatgtgt tcatggcgac gaccgccgat gcgctggtcg
     2401 aggaattggt ggcggtgctg ggcgcaggga acaaagcgcc gtggaagatt cgggcgccca
     2461 caaagcggaa agaggcgagc ggcgctgccg tgacactgga tgggatcgcg tcggctttgc
     2521 gcagcgcctt caacgatccg gaaaaggtga cgttctcgct tttgaatcgc ggctggccgt
     2581 ccgatatttg gccgttcaaa agcgcgcttg cctatctcgg caaggacggc ggcggggggc
     2641 tcggttcggg gcccggtctg tcggtggggg ccgccgtcgc gctcgaacgc atgggccgct
     2701 atgcggtttc cgtgctgggc gatggagatt tctgcatggg cgcgaccgcc atctggaccg
     2761 ccgtacacca tcgcattccg atgttgatcc tgatcgacaa caaccgctcc tatttcaatg
     2821 acgaactgca tcagcagaat gtcgcccgcg cgcgcggccg cgatgtgcgc aatcgctgga
     2881 tcggtttgcg gatggaggat cccatgcccg acatcgcgac gctggcgcgg ggacagggcg
     2941 cggtcggcat cgggccggtg acgcgcgccg ccgatgtggc ggcggcgttg gaagaaggcg
     3001 tgggcgtgct gaaacaggga ggggtatgcg tgatcgattt ccacgtcgat cccggcgaag
     3061 accgcacctc cgctgcgtcg gtgggtcagc gcaagaccta ggtcttggtg aatgccgtct
     3121 ttatgtccgc gttaaatacg gcacggcaaa atcatgcgcc cttaaacggc tgccgcgtat
     3181 tttgcggtgc ggaactgcgg gcaaccgatg ctgctcaaca tcgtcaatgg ctatgtctgt
     3241 cacaattgca ccgatgtcga aaacgccaag aaaggtgtcg atcccgccca tccgaaaaaa
     3301 gattctgctc cgcaatcgcc taccaaaact ggaatcgcgc aaagcaatcc ggcggttatt
     3361 ctgagcggcg cactggccaa gaccggtgcc gcgaaacccg caggatcggg attgacgaac
     3421 caagctccaa acgcgccgcc gcttgcaatc cccgagaccg gcgattctgt gaacctgtcg
     3481 atataagtgt cacgcgcatt cgcgcgcact aagacaaatc ggatgcggaa ttatcggagc
     3541 cggtgagctc ctttcggcga tgacgcatct ccagcgcccg catcagttct tggacgatct
     3601 gacgggagtt gcgtgtcgcg accgctgctg ccatcccgtc attgaggaag acgactctgc
     3661 tgtcctggcc ggctggcccc ggcaccgaaa ggaacacggg aatcttcttc tccatgagcg
     3721 cggcgatgta tgtgacgaac ggttcccacc gcacgctctg caatgaggcg acaagctgga
     3781 atttcagccc gacccgttgt tcgaaatggc tcggtatccc ggtttgcgtc atgctgacaa
     3841 gaattccact tcggtccggg cgccaggcat cgtctaaggg aagctttcgc cagccgcaaa
     3901 accatgttct gcatacggac ggcctcgtct cataaatgcc gcatcccgat gccgggctac
     3961 aatgtgtgca aagcataccc tccggctttt tcaattcggg actctgaatc gccaaatgcg
     4021 tgcagcagac ggtgcatccg ccgcagtccc ggttgggaac gaggctctct gtcacgattt
     4081 caccgatcag ggcgaatggt tcgcctcacc aaaacccacg cgttacgcgc tattttctgt
     4141 ggtctttgga cgcaacgtcc ctattcgggc aggtcggagc gcaatactcg tgacgatgcg
     4201 cttacgcagt tgatgaagcc ggccgggaag attttacgag acgatcccgg ttcgctttaa
     4261 cgacacgcgc caaaccaatc ggcctagcgg aatgaacgct tcggtatcaa cgcgacgtac
     4321 tttgtaagcg tctacctgtt ccccaagctt ggcaaccgtc tgtggccttg gccggctagg
     4381 gctagacggg ttatcgctgc taaaaactgt cgtggttcgg tcaagcgggg ccgtgacgcg
     4441 aaaggacatc aggtctagaa cgagaggcgc tctggatggc tggtggaaat ttggggtcga
     4501 aagcaccatg ttgggagcgt ggcgcggcga aggcgccgat cccgaaaggt accggatgca
     4561 cctctattgc accgtaattc gtaccaaata gtcttgcgcg cttgatatgt tttccgtgta
     4621 aaatcaatga gataaccgct ctgcccgcct caaaatgagg gggaggggga ccaaggacca
     4681 aaaccgatgt tcatccagac cgaaaccacg cccaatcccg ccacgttgaa attccttccc
     4741 gggcgcgagg tcatggcgtc gggcacggtg gatttccccg atgccgaggc cggcaagcgc
     4801 tcgcctttgg cgagccgcct tttcgccatt gccgaggtgg agcgggtgtt cttcggttcg
     4861 gacttcgtca ccgtgaccaa acgcgagagt gatgccatcg actggcggca tctgaagccg
     4921 atgatcctgg gcgcgatcat ggagcatttc acccgcggcc ttccgctttt cgacagtgag
     4981 ggtgaagccg aggccgagcc catctatgag ggcgaggaag gggagattgt cgcccagatc
     5041 aaggaactga tcgagacgcg cgtgcgcccg gcggtggcct cggacggcgg cgacatcatc
     5101 ttcaaaggct tcgatggggg atcggggacc gtgttcctgc atctgcaggg ctcatgttcg
     5161 ggctgcccct cctcgaccat cacgctgaag aacggcatcg agaacatgct gcgccattac
     5221 gtgccggagg tttccgctgt cgaggcggtc tagacccacc cgttcaaccg cattgaccgt
     5281 cccgccgctc cctgggaaag cttgcgcgca aagctaaggt ccgttgaggc cgtgaaattc
     5341 tattcccagg tcgcccagga ccgcttcctt ctggagaatt tctttcgcgg acgccggggc
     5401 ggcgtgttcc tcgatatcgg cgcctatgat ggcgagaccc tcagcaacac cgctttcttc
     5461 gagcgcagca tgggctggac cgggctctgc gtcgagccgt tgccctcggc cttcgccaaa
     5521 ctcaccgccc ggcggaaagc gctgtgcgaa tgcgtgtgcg tcgccgattt cgagggcgag
     5581 gcggatttcc tcgaatccga taccgccagc cccgacgaga tgatgctctc cggcatgccg
     5641 gcgcatttcg atccgcgcca tgtcgcgcgg ctggacgagt gcgccaccac gcgcgtcacg
     5701 cgccgcgtgc cggtgacgaa gctttccacg cttctgacca agcatcggct gttcgacatc
     5761 gactattgct ccatcgatac ggaaggcgcc gaactcgcca tcctgtcgga tttcgatccc
     5821 gaacgattcc gcatcaagct cttcaccatc gagaataatt gggacgatcc acgaatcccc
     5881 gatctgatgg cgcagaaggg ttacgagctt ttcgcgaagc tcgaatggga caatgtctac
     5941 aggcggcgcg acgtgaagcc cctgccgcgc accagcgtga tctgcgcggt gtggcatggc
     6001 gatccgaacc ggcacgcatt gctgcgcggc catgccgaga atctggcgcg gcagagcgtg
     6061 ccggtcgaac ccatctatgt tttcgacaat ggcgatgcgc cgccgccctg gctggaggcg
     6121 cgcgccatca gcgtgaagga accgctcacc atctatcagg cgtggaacgt ggcgttgtcg
     6181 ctggtggcca cgccgcttgc gatgaatctc aatctcgacg accggctggc gcccgatgcg
     6241 attggcatgc tggagcagga acttctgaag aatcgcgcgg tcgcggcggg cggggattgg
     6301 aaggtttgtt acgcgcaggc cgaaaccgac gatgtcgcgc cctgctttcc ggtctctggc
     6361 gtgcccttcc ttccggcgtg gccgcccgcg cgcgggagcc tgacgcggct tggcagcggc
     6421 accggcgaac gcgggaccta tggcccggcc accatctggc ggatggatgc ccatgtcgga
     6481 gcgccgcgtt atccctggcg cttacgcgac ggatcgctcc tgcgcgccag tgccgacctc
     6541 gcctggtggc agatgctgca gagcaatccc aatttcaaaa tactcgcgat tcccatggtg
     6601 atcggcaatt atcacagcca ccccgaaggc caggccgaat tccgtgaagc ggacgagcgg
     6661 gcggccatgg ccgaccccgg cgtatcgctg acttgatttt cgcacgccga aacgtgctct
     6721 aatcgggcac gggtaggggg atcattgacc gtagctcatg tctacacggt cgcgttcgag
     6781 ggaatcgcgg cgcgcgaagt cgacgtgcag gtccatatcg gtgagggcgg cgcgggggtc
     6841 ttcactgttg tcggcctcgg cgataaggcc attggcgaat cgcgcgaacg cgtgcgcgcg
     6901 gcgctggccg ccgtcgggct cgcactgccg tggaagcgga tcacggtcaa tctggcgccc
     6961 gccgacctgc ccaaggaagg cagccattac gatctgccca tcgccctggc cctgctcgcc
     7021 gccatgggtg tcctgccggt gagcgagatg gcgggctata ccgcgatggg cgagttgtcg
     7081 ctggatggcc agatcacggc ggttgcgggt gtgctgcccg ccgctatcgc cgcggccgag
     7141 gccgagcgcg gcctgatctg ccctgcggcc tgcggcgcgg aagccgcttg ggccggcggg
     7201 gtggagatca tcgcggcccc ttcgctgatc gcgctcgtca accacatcaa gggcgcgcag
     7261 gtgcttggcg ttcccgaggc gcgcctggcg gaaagcgcgg tttcgattcc agacttgaag
     7321 gatgtgaagg gccaggagac ggcaaagcgc gcgctcgaaa tcgccgcggc cggcggccat
     7381 aacatgctga cgttgtggtc tagttacccg gcaagctgct tctcgaatca gagtttttca
     7441 tgagccgtga tcccgcacac atcttgctaa gcactaacaa ttctgaggtg ccgcaacttc
     7501 caaagcggaa gaccgggcgc tttattaccg agttcatcgc ggtattcttc tggatatacg
     7561 cactcacgaa actcttcatt tttgatctag acatttggat catcaatttg gtttcgcctc
     7621 agttcgcatc gatactgaat tacaaacttc cgattatcct cgttggcata tcgatagcca
     7681 tagtgacgac gcgaagcgcg agactgggtt taagcgcact ctacatcgcg ttttatcctt
     7741 tagtgatttt gagctggaag attccgcgtt ttgtttggca tcaaaaaagt tggctgttag
     7801 catttg
//
LOCUS       DASYEG010000157         6441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3673969, whole genome shotgun sequence.
ACCESSION   DASYEG010000157 DASYEG010000000
VERSION     DASYEG010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3673969"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(402..1673)
                     /gene="pseC"
                     /locus_tag="VGM72_07225"
     CDS             complement(402..1673)
                     /gene="pseC"
                     /locus_tag="VGM72_07225"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: protein motif:HMM:TIGR03588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HEY4265092.1"
                     /translation="MSRTSAAAPAFAASEYSASPASVHRFLPYGRQCIEDDDIEAVTA
                     ALRGEYLTTGPLVERFEQHLASATGARAAVACSNGTSALYLAGRALGLGKGHTAVVPA
                     ITFLATASAPHLLGADIVFSDVDPESGLMRARDLESALARCPGGRADAIFPVHYAGQS
                     CDMGAIADLARARGAKIVEDAAHALGTAWIGDDGALVPVGANNWCDLTIFSFHPVKTI
                     AMGEGGAVTANDPELIARLKQGRNHGITRDPGQFIRADAAFDANGAPHPWYYELEAPS
                     FNFRTCDINCALGISQLAKLRRFVETRRALVAAYDSLLSPFAPLVRPLKRDPRSSTAW
                     HIYPMRVDFAKLGLSRADVMRALTRDDIGTQVHYIPVHRQPYYAARYGIPDLPGADAY
                     YAATLSLPLFPAMTGDDAARVVAALKHHLKL"
     gene            complement(1673..2761)
                     /gene="pseB"
                     /locus_tag="VGM72_07230"
     CDS             complement(1673..2761)
                     /gene="pseB"
                     /locus_tag="VGM72_07230"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HEY4265093.1"
                     /translation="MAKPALAFQDNTAAPIAQPASTLRDFSQPWLDLNDKSVLVTGGT
                     GSFGKHFLKTVVERYKPRRLIIFSRDELKQFEMAQAFPAEKYPFLRFFIGDVRDRDRL
                     ELALRDVDYVVHAAALKQVPTAEYNPFECIRTNVFGAENLVYAALHRNVKKVIALSTD
                     KAANPVNLYGASKLASDKIFVAANNLAGAEGTRFAVVRYGNVFGSRGSVVPFFRKLAE
                     DGAASLPITDERMTRFWITLTQGVNFVLSSTEMMRGGEIFVPKIPSTTIVDLASLLGP
                     KLAQHNVGVRPGEKLHETMIPADDSRWTVEIDDRYIILASFAAAAREAYLNRGGKPVE
                     EGFQYSSDSNPERLDARGLQALLAQAFL"
     gene            complement(2810..4810)
                     /locus_tag="VGM72_07235"
     CDS             complement(2810..4810)
                     /locus_tag="VGM72_07235"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265094.1"
                     /translation="MRTAAQRQPIAPPVPELARLNLFALFHLNLAFSSIEESDRAAVI
                     ERCYWPLLALCAAHGPIGIEASGYTLEQIEMRDPAWIRRLGDLIADGKAELIGSGYAQ
                     LIGPLAPATLVAKNLGIGNAVYRQLLGTSPTLALVNEQAYAGGLVGHYLDAGYKALLM
                     DWDNPAAQHPEWSAESRYLPQTARGADGRTIGLLWTNTVAFQKLQRYAHGDMEFEDYL
                     DFVLAQQGASPRALCVYASDGEIFGFRPGRYRTEERLEDGEWTRLGAAFSALSSERGM
                     RLVTPSGALTAMKNPGAEPLRLETAACPVPVKKQRKYNLTRWAVTGRDDIAINAACQR
                     IHAALLRGHAPETDWKTLCRLWASDFRTHITQARWDAYCAELSAMEARLGTSPTLSPP
                     KAHPPECAARITGVERRFIDIETRSVRVRLDRRRGLAIARVAFDGDAPIIGGLPHGHF
                     DDIALQADWYTGDCVFEEPGEPKITDLDWAETELWHEDGDVLVCGRIQTPLGPIEKTL
                     RVSANEPRIGFDMTFHWSEWRKGALRLGHITLLPEAFDWQSLALTTHNGGFAPERFAL
                     SGTQIDHGAPVSFLVSASCGLGMTEGWAELSDGKRTVRVEVEREIAPLLGLLTHRQMG
                     GSLFCQLALSMLELDDTRRPAPYLPGPRRVRFSILGVTQTLP"
     gene            complement(4807..6024)
                     /locus_tag="VGM72_07240"
     CDS             complement(4807..6024)
                     /locus_tag="VGM72_07240"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY4265095.1"
                     /translation="MTRTLLIISGGIEAVDAIKRARDMGLHVVVSDIAADAPGFAHAH
                     DRIIADVYGAEETLQAVERYVREQRPIDGVLCVAADAPVTAARIAGKLKLPGISVATA
                     KLACDKLAMKRRFKECGVPVPWFAAVASAAELAAIVAERGADLVIKPVDSRGSRGVQR
                     LSRSPDIAGAFALAQKHSPTGRVMVEAYLDGPQISTESIVTRGRCHTPGFADRNYELL
                     EVHAPFFTENGGDLPSHLPQDIQQKVKDVIAGAATVLGVTDGTVKGDVVVHRGQPYVI
                     ELAARLSGGFFCTLEIPLNTGVDFIGCAIRVALGETIAAAELEPSRQQPVIQRYAFPK
                     AGRVIAIRGADTAKNLPGIAGIIVTARPGDIIPPAGDKRPSAAMVLATGTTHAAALAA
                     AHAGVAAIEIETQ"
     gene            complement(6021..>6441)
                     /locus_tag="VGM72_07245"
     CDS             complement(6021..>6441)
                     /locus_tag="VGM72_07245"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4265096.1"
                     /translation="METNLRGPFAIAQETLPAMTHKGWGRIVNIVSIGGQWGGMRQVH
                     YAAAKAGLINFTHSLAKLHSRDGITANAVSPGLIATDMTEKELRAKAGRAKAAQIPAG
                     RVGTPEDVAAAVVFLCSEAAGYITGHTLNVNGGLYFQ"
BASE COUNT         1230 a         1934 c         2114 g         1163 t
ORIGIN      
        1 catcggcaac ctggtggatg ccgacatggc ggtggaaagc gccaaactgc aatccttgca
       61 ggtcaagcag caattgggcg tgcaagccct gtcgatcgcc aattcggcgc catcgatcac
      121 gctgtccctg ttccgataag aaaaaaatcg cggggccggc gcatgcgggc ctcgcgattt
      181 tccgccggga tcgatgggaa ttcgattttg gtggacgtcc caatatcaag ggaagacggg
      241 acgcaagaga tctgcgagag gactatggcc gatccgctct cctatccgac aaaacgggat
      301 gggaggatgg gtttgaacct agccccctcg caggtttttc tgttttccgg caaagccggc
      361 gaagcgggag gggcacgttc cgacacgtac gctcgcgcga atcaaagctt cagatgatgt
      421 ttcagcgcgg ccacgacacg ggccgcatca tcgccggtca tggcggggaa aagcggcagg
      481 ctgagggtcg ctgcataata ggcatccgcg cccggaagat cggggatgcc gtaacgcgcg
      541 gcataatagg gctggcggtg aactgggata taatgcactt gcgtgccgat gtcgtcgcgg
      601 gtcaaggcgc gcatcacatc ggcgcgggat aatccgagtt tcgcgaaatc cacgcgcatc
      661 ggatagatat gccaagcggt cgacgaccgc ggatcgcgct tcagcggacg gaccagaggc
      721 gcaaacggcg aaagcaatga atcataggcg gccacgagtg cgcgccgcgt ctcgacgaag
      781 cggcggagct ttgcaagctg agagattcca agcgcgcaat tgatgtcgca ggtccggaaa
      841 ttgaagctgg gagcttccag ctcgtaatac cagggatggg gtgcgccgtt ggcgtcgaat
      901 gccgcgtcgg cgcgtatgaa ttgtccgggg tcgcgggtaa tgccgtgatt gcggccttgc
      961 ttgagtcgcg cgatcagctc cggatcgttc gcggtgaccg cgccgccttc gcccatcgca
     1021 atggtcttca ccggatgaaa cgaaaaaatg gtgaggtcgc accagttgtt cgcgccgaca
     1081 ggaacaagcg cgccgtcgtc gccgatccag gccgtgccca gcgcatgtgc ggcgtcttcc
     1141 acgattttgg cgccacgcgc acgtgcaaga tctgcaatgg cgcccatgtc gcagctctgc
     1201 ccggcatagt gcacggggaa tatggcgtcg gcgcgcccgc cgggacagcg cgccagggcg
     1261 ctttccagat cgcgcgcgcg catcaatccg gattcgggat cgacgtcgct aaaaacgatg
     1321 tccgccccca gaagatgggg cgcgctcgcc gtggcgagga aggtaatggc tggaaccacg
     1381 gccgtgtggc ctttgccgag accaagggcg cggcccgcca gataaagcgc ggacgtgccg
     1441 ttggagcatg cgacagcggc tcgcgcgccg gtggcgcttg ccaaatgttg ctcgaagcgt
     1501 tcgaccaatg gaccggtggt gaggtattca ccgcgaagtg cggcggtcac cgcctcgatg
     1561 tcatcgtctt cgatgcactg acgcccatag ggcagaaagc gatggacgct tgcaggcgaa
     1621 gcggaatatt cggacgccgc aaacgcgggt gctgctgcgc tcgtcctgga catcaaagga
     1681 aggcttgcgc gagcagtgcc tgaagcccgc gcgcgtcgag ccgctcggga ttgctgtcgc
     1741 tcgaatattg aaagccctct tccaccggct tgccgccgcg attgagatag gcttcgcgcg
     1801 ccgctgccgc aaagcttgcc aggatgatgt agcggtcgtc gatttcgacg gtccagcgcg
     1861 aatcgtcggc cgggatcatg gtctcgtgca gcttctcgcc gggccgcacg ccgacattgt
     1921 gctgtgcgag cttaggaccg agcagggagg cgagatcgac aatggtcgtg gagggaatct
     1981 tgggaacgaa gatctcgccg ccgcgcatca tctccgtgct cgacaagacg aaattcaccc
     2041 cttgcgtcag cgtgatccag aagcgtgtca tgcgttcgtc ggtgatggga agcgaggccg
     2101 cgccatcttc cgccaatttg cggaaaaacg gcaccacgct gccgcgcgag ccgaacacat
     2161 tgccatagcg caccacagcg aaacgcgtcc cttcggcgcc cgccagattg ttagcggcga
     2221 cgaagatctt gtcggacgca agcttggaag cgccgtagag attaaccgga ttggcggcct
     2281 tgtcggtcga aagcgcgatc accttcttca cattgcggtg gagcgcggca tagacgaggt
     2341 tctctgcgcc gaacacgttg gtacggatac actcgaacgg attgtattcc gcggtcggca
     2401 cctgtttcag cgccgcggca tggaccacat agtccacgtc gcgcagagcg agttcgagac
     2461 ggtcgcggtc tcggacatca ccgataaaaa accgcaggaa aggatatttc tcggccggga
     2521 acgcctgcgc catctcgaat tgcttgagct cgtcgcgcga gaaaatgatg agacggcggg
     2581 gcttgtagcg ctcgacaacg gtcttcagga agtgcttccc gaacgaaccc gtgccgccgg
     2641 tgaccagcac ggatttgtcg ttcagatcga gccagggctg gctgaaatcg cgcaacgtgc
     2701 tcgccggttg ggcgatggga gccgctgtgt tgtcctgaaa tgcgagggca ggtttggcca
     2761 tgcgggcttc gtccttcgaa gatggtggac gaatggtgca ccgaattagt tagggaagcg
     2821 tttgcgtgac ccctaaaatt gaaaaccgaa ccctgcgcgg ccccggcaaa taaggagccg
     2881 gccggcgggt atcgtccaat tccagcatcg aaagcgccaa ttggcagaat aggctgccgc
     2941 ccatctgtct gtgggtgaga agtcccagca gaggcgcgat ctcgcgttcg acctccacgc
     3001 ggactgtgcg tttgccatcg gagagttccg cccaaccctc ggtcatgccg agcccgcagc
     3061 tcgccgagac caggaaggac accggcgccc catgatcgat ttgggtgccg gaaagcgcga
     3121 agcgttcggg cgcgaaaccg ccattatgcg tggtgagcgc gaggctttgc caatcgaaag
     3181 cctccggcaa aagcgtgata tggccgagcc ggagcgcgcc cttacgccat tcggaccagt
     3241 ggaacgtcat gtcgaaaccg attctgggtt cgtttgcgga cacgcgcagc gttttctcga
     3301 tcggtccgag tggcgtctgg attcggccgc agaccagcac atcgccgtct tcgtgccaaa
     3361 gctcggtctc ggcccaatcg agatcggtga tcttgggttc gccgggttcc tcgaagacac
     3421 agtcgccggt gtaccaatcg gcctgcagcg cgatgtcgtc gaaatggccg tgcggtagcc
     3481 cgccgatgat gggtgcatcg ccgtcgaaag ccacgcgcgc aatcgccagg ccgcggcggc
     3541 ggtcgagccg cacgcggaca gaacgcgttt cgatatcgat gaagcgccgc tcgaccccgg
     3601 tgatccgcgc agcgcattcc ggaggatggg cctttggagg ggacaatgtg ggtgacgtcc
     3661 ccagcctcgc ctccatcgcc gaaagctcgg cgcaataggc atcccagcgc gcctgcgtga
     3721 tgtgggtgcg aaaatcgctc gcccacaagc ggcaaagcgt tttccaatcc gtctccggtg
     3781 catggccacg caagagcgcg gcatgaattc gctggcaggc ggcattgatg gcaatatcgt
     3841 cgcgacccgt caccgcccag cgcgtgagat tgtatttgcg ctgttttttc accggcacgg
     3901 ggcaggcggc ggtttccagg cgcaacggct cggcgccggg atttttcatg gccgtaagag
     3961 ctccggaggg tgtgaccaac cgcattcccc tctccgatga caacgcggaa aaggcggcgc
     4021 cgagacgtgt ccactcgccg tcttccaggc gttcttcggt ccgatagcgg cctggacgga
     4081 aaccgaaaat ctcgccatcg ctcgcataga cgcagagcgc gcgaggagaa gctccctgct
     4141 gtgccagaac aaaatcgaga tagtcctcga actccatatc gccatgcgca taacgctgca
     4201 gtttctggaa ggcgacggtg ttggtccaca gaagtccaat agtgcgcccg tccgcgcccc
     4261 tcgcggtttg cggcaggtag cgcgattccg cgctccattc gggatgctgt gcggcgggat
     4321 tgtcccaatc catcagcaat gccttgtagc cggcatcgag ataatggccg accagcccgc
     4381 ccgcataagc ctgctcattg acgagggcga gtgtcggaga cgtgccgagc agctggcggt
     4441 agaccgcatt gccgattccc agatttttcg ccaccagcgt cgcgggcgcc agcggcccga
     4501 tcaattgcgc ataaccggac ccgatcagct cggcctttcc atcggcgatc agatcgccca
     4561 agcgtcggat ccaggcagga tcgcgcatct cgatttgctc cagcgtatag ccggaggcct
     4621 cgatcccgat ggggccgtgg gccgcgcaga gcgcgagcag cggccagtag cagcgttcga
     4681 tgacggcggc gcggtcgctt tcctcgatgg aagaaaaagc gaggttcaga tggaacagcg
     4741 caaaaagatt cagcctcgca agttccggaa ccggcggcgc gatcggctga cgctgcgcgg
     4801 cggtcctcat tgggtttcga tctcgatggc cgcaacaccg gcatgcgcgg cggcgagggc
     4861 ggcggcgtgc gtcgttccgg tcgccagcac catggcggcg gaggggcgct tgtcgcccgc
     4921 cggaggaatg atgtcgccgg gtctcgccgt gacaatgatc ccggcaattc ccggaaggtt
     4981 tttggcggta tcggccccgc gtattgcgat cacccggccg gctttcggga acgcatagcg
     5041 ctggatcacc ggctgttgcc gggaaggctc caattccgca gccgcgatgg tttcacccag
     5101 tgccacgcga atagcgcagc cgatgaaatc cacgcccgtg ttgagcggaa tctcgagcgt
     5161 gcagaagaat ccaccggaga gacgcgccgc cagctcgatg acatagggct ggccccgatg
     5221 gacgacgaca tcgcctttca cggttccgtc ggtgacgcca agaaccgttg cggcgcccgc
     5281 gatcacgtcc ttcacctttt gctgaatgtc ttgcggcaga tggctcggca ggtcgccgcc
     5341 gttctcggtg aagaatggcg catgcacttc cagcagctcg taattgcggt cggcaaatcc
     5401 cggcgtatgg cagcgcccgc gcgtgacgat cgactcggtc gatatctgcg gtccgtcgag
     5461 ataggcttcc accatcacgc gccccgtcgg agagtgcttc tgcgcgagcg caaaggcgcc
     5521 agcgatatcg ggagagcgcg aaagacgctg cacaccgcgg ctgccgcgac tgtccaccgg
     5581 cttgatgacg agatcggccc cgcgctcggc gacgatggcg gcgagttcgg ccgcgcttgc
     5641 aacggcagca aaccatggca cgggcacgcc gcactccttg aagcggcgct tcatggcgag
     5701 cttgtcgcaa gcgagcttcg cggtggcgac ggagatgccc ggcagtttca gttttccggc
     5761 gattcgcgcc gcggtgacgg gggcatccgc cgccacgcat agaacgccat cgatggggcg
     5821 ctgttcgcgg acatagcgtt cgacggcttg aagcgtctct tccgcgccgt agacatcggc
     5881 gatgatccgg tcgtgcgcat gggcaaagcc cggcgcgtcg gcggcgatat cggagacgac
     5941 gacatgaagc cccatgtcgc gcgcgcgctt gatggcgtcg accgcttcga tgccgccgga
     6001 gatgatgagc agagtcctgg tcattggaag taaagcccac cattgacgtt gagggtgtgg
     6061 ccggtgatgt agccggccgc ttcggagcag aggaaaacca ccgccgcggc gacgtcttcg
     6121 ggcgtgccga cgcggccggc cggaatctgc gcggctttgg cgcgtcccgc tttggcgcgc
     6181 agttccttct ccgtcatgtc ggtggcgatg aggcccggcg agacggcgtt cgcggtgatg
     6241 ccgtcgcgcg aatggagctt cgcgagcgaa tgggtgaaat tgatcagtcc ggctttggct
     6301 gcggcgtaat gcacttgacg catgccgccc cattggccgc cgatggaaac gatattgacg
     6361 atgcgtcccc aacctttgtg tgtcatggcg ggcaacgtct cctgcgcgat cgcgaaaggc
     6421 ccgcgcagat tggtttccat c
//
LOCUS       DASYEG010000158         2203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2513756, whole genome shotgun sequence.
ACCESSION   DASYEG010000158 DASYEG010000000
VERSION     DASYEG010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2513756"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..75)
                     /locus_tag="VGM72_07250"
     tRNA            complement(1..75)
                     /locus_tag="VGM72_07250"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Gly,seq:gcc)
     gene            230..1210
                     /gene="trxA"
                     /locus_tag="VGM72_07255"
     CDS             230..1210
                     /gene="trxA"
                     /locus_tag="VGM72_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY4265097.1"
                     /translation="MASLIDPTGRPLKMSRDNAPAKAAPGDAAAPAVAGSPIKDATLA
                     SFAADVLDASMDVPVIVDFWAPWCGPCKQLGPTLERLVAETKGAVRLVKVNIDENPEI
                     AQQLRIQSIPTVYAFRNGQPVDGFMGAIPDSQLAQFVQGLIGPGGGEAAAAVEGTLAL
                     AADALAKHDLTAAAQIFGQVLQEDPGHPKAVAGLARCYLESGDLERAKKTLGLVRPDG
                     ASDESIRAVEAELALKERAASAGELEPLRARIAADPADLQARFDLALGLDAKGAREEA
                     IDELLEIMRRNRKWNDDAARKQLVTLFDAMGPADPRTIAARRRLSSLLFS"
     gene            1220..1891
                     /locus_tag="VGM72_07260"
     CDS             1220..1891
                     /locus_tag="VGM72_07260"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY4265098.1"
                     /translation="MPALRRYRTLSDLPAVIPVFPLTGVILLPRTKLPLNIFEPRYLA
                     LVDDAMDHYRLIGMIQPRNAGMEPAKNPPLTDVGCVGRITEYSETDDGRYLIALTGIA
                     RFQVSGEREAHTPFRQIAADYTLFAGDLLSTEDPPVPRARLLGALKPYLSDRAMQTDW
                     KSIEEAPAETLVNALCMLCPFEPSEKQALLEAPGLKERVDALITLLEIANASAMPSGG
                     SPSIN"
     gene            1903..2088
                     /locus_tag="VGM72_07265"
     CDS             1903..2088
                     /locus_tag="VGM72_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEY4265099.1"
                     /translation="MDVDPKLLEILVCPQTKGPLIYDRERQELISKKAGLAFPVRDGI
                     PVMLAEEARALSESEQN"
BASE COUNT          403 a          705 c          680 g          415 t
ORIGIN      
        1 tggagcgggc gaggcgattc gaacgcccga ccctaacctt ggcaaggtta tgctctaccc
       61 ctgagctacg cccgcgctcc aagcaagggt ggggttattg cgcagcccgc cataagtttc
      121 aatagccgcg gggcggcggc gctcgtggcc attccaacga tgctacaatg gcttggcttt
      181 ttcccatcaa gcacccatgt tgagggcgaa aatcacccaa gggcatatca tggcttcgct
      241 tatcgacccg accggccggc cgctgaaaat gagccgggac aatgccccgg ctaaagccgc
      301 gcccggtgat gccgcggctc cggcggtggc cggctcccct atcaaggacg caaccctcgc
      361 gagtttcgcg gccgatgtgc tcgatgcctc gatggacgtc cctgtcatcg tggatttctg
      421 ggcgccctgg tgcgggccct gcaagcaatt gggtcctacg ctggaacgcc tggtggccga
      481 aaccaagggt gctgtgcgcc tcgtcaaggt gaatatcgac gagaaccctg aaatcgcgca
      541 gcaattgcgc atccagtcga ttccgacggt ctatgccttc aggaacggcc agccggtcga
      601 cggcttcatg ggcgccatcc ccgatagtca gcttgcgcaa ttcgttcaag gtctgatcgg
      661 cccgggcggc ggcgaagcgg cagccgcggt cgagggcacg cttgcgctcg cggcggatgc
      721 gctcgccaag cacgatctca cggcggccgc tcagatcttc ggtcaggttc tgcaggaaga
      781 tcccggccac cccaaagccg tggcgggatt ggcgcgctgc tatctcgaaa gcggtgatct
      841 cgaacgcgcc aagaagacgc tcgggctggt gcgccccgat ggtgcatccg acgaatcgat
      901 ccgcgcggtg gaagccgaac tcgcgctcaa ggaacgcgca gcctcggccg gggagctcga
      961 acctttgcgc gccagaatcg ccgccgatcc ggccgacctg caggcgcgct tcgatctggc
     1021 gctcggcctc gatgccaaag gcgcacgcga ggaggcgatc gacgagcttc tggagatcat
     1081 gcggcgcaat cgaaaatgga acgacgatgc ggcgcgcaag caattggtga cgctgttcga
     1141 tgcgatgggg cccgccgacc cgcgaaccat cgccgcgcgg cggcggctgt cgtccttgct
     1201 gttctcctga tttttcccga tgcccgcgct gcgccgctac cgcacgctct ccgatcttcc
     1261 ggcggtcatt ccggtgtttc cgttgacggg cgtgatcctg ctgccgcgca ccaagctgcc
     1321 gctgaacatt ttcgagcccc gctatctcgc actcgtcgat gacgccatgg atcactatcg
     1381 cctgatcggc atgatccagc cacgcaacgc gggtatggag ccggccaaaa atccgccgct
     1441 caccgatgtg ggttgcgtcg gacgcatcac cgaatacagc gagaccgacg acggccgcta
     1501 tctgatcgcg ctgacgggga tcgcgcgctt tcaggtgtcc ggcgagcgcg aggcgcatac
     1561 gccgtttcgc caaatcgccg cggattatac tttgtttgcc ggcgatcttc tgtctacgga
     1621 agatcctccc gtgccgcgcg cgcgtctgct tggcgcgctt aagccctatc tttcggaccg
     1681 cgccatgcaa accgattgga aatcgatcga agaagctccg gccgaaacgc tggtgaacgc
     1741 gctgtgcatg ctctgcccct tcgagcccag cgagaaacag gcattgctcg aagcacccgg
     1801 cctgaaggaa cgtgtcgatg cgctgatcac tcttctggag atcgccaacg cctcggcgat
     1861 gccgtcgggc ggctctccct ccatcaattg aggttttcat tcatggatgt cgatcctaaa
     1921 ctgctggaaa tcctggtttg tccgcagacc aagggaccgc tcatctatga tcgcgagcgt
     1981 caggaattga tcagcaaaaa ggcggggctt gcctttcccg tccgcgatgg cattcccgtg
     2041 atgctggccg aagaagcccg cgcactttcc gagagcgaac agaattgagt gcggcacccg
     2101 cgccctggcc ggtggaactg agactgaacg ccgccaagga tgttctgacg atccgcttcg
     2161 acaatggcga ggcgttcgac ttggcggcgg aatatctgcg cgt
//
LOCUS       DASYEG010000159         6823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4400158, whole genome shotgun sequence.
ACCESSION   DASYEG010000159 DASYEG010000000
VERSION     DASYEG010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6823
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4400158"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="VGM72_07270"
     CDS             complement(<1..1011)
                     /locus_tag="VGM72_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY4265100.1"
                     /translation="MEFERSDEQNVFAESVRRFAVAKLADGALARAHSNAYPWDIARL
                     LAEQGLMGITIPEQDGGIGGTLMDAVIAIQEVALVCPKSADIVQAGNFGPIRTFAEYA
                     SPEQKARFLPDLLAGRKLISLGMTEPDAGSAVTDLHTSAKRDGDAFVINGGKIFTTHS
                     TEAGLFLIYVRYGPGVGGIGSVLVERGTAGFAIGEPSSFMSGEQWAPLYFDNCRIPAE
                     NMLLGEGGFKKQIAGFNVERLGNAARALALGRHAFNAARDYALIRRQFGRLLCEFQGL
                     QWKFAESAIKLDAAQLLLYRAIANAKDGLPSAYDAAAAKLACNLAGWEVSNEALQIMG
                     GMG"
     gene            complement(1074..2405)
                     /locus_tag="VGM72_07275"
     CDS             complement(1074..2405)
                     /locus_tag="VGM72_07275"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY4265101.1"
                     /translation="MTIVQTLAEFAADFEKRQVPGQIVHHAKRAVIDWYAALLPGAIS
                     APAIFLERALAGELDRGTSKLALGRRATPRAAALINGTAAHTAEVDDIFREAIYHPGA
                     PIIAAVLAATQEQGSNGATFLKGVIAGYEISTRIGVAMGRAHYRFWHNTGTIGTFGAA
                     AAAAVIYGLDPERFAHALANAATFAAGLQQAFRTGSMSKPLHAGRAAEGGLLAALAAR
                     EGVTGSLDVLEGDVGFGRAMGGDPDWNAALANLGHEFHIAKVTFKNHACCGHAFAAID
                     GALELQRKLGVAARDIAWVTVETYGEALQVAGNPRPMTPGEARFSIPYVVATALVHGS
                     VRLAAFDAERIADPMTCDLMERIALKRDGDIDAGFPGRRAARVGIETRDGRREQFLQP
                     TRKGDPELPLTDYELEEKYRELAIPVIGESKAAILLEHLWRLDSLPHVDLH"
     gene            complement(3185..4348)
                     /locus_tag="VGM72_07280"
     CDS             complement(3185..4348)
                     /locus_tag="VGM72_07280"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HEY4265102.1"
                     /translation="MSDPTGFPFDELAGERGGGDNPYGKYSTGILPSHVLRRLIRGRR
                     EVLAAEEVIEEQIQPASLDLRLGANAYRIRASFLPGRDARIADKLSELAIHEIDLRPG
                     AVLETGCVYLVPLLERLELSFRVSGVANPKSSTGRLDVFTRLITDHAQAFDRVEEGYH
                     GPLYAEISPRTFPLLVRKGSRLNQLRLRHGSPQFSDTQLKRLHEEVGLVDGPADIEGG
                     LALSVDLRGETLTRQVGWRARRHAGVIDIDVRGAHDPLDFWDPIYARKGTSIILDPDE
                     FYILASREAISIPPDYAAEMMPFNPLVGEFRVHYAGFFDPGFGHESGHGRGARAVLEV
                     RSREVPFVLEHGQTIGRLVFERLTDTPPQVYGQVLGSNYQRQGLKLSKHFRLP"
     gene            complement(4451..4894)
                     /locus_tag="VGM72_07285"
     CDS             complement(4451..4894)
                     /locus_tag="VGM72_07285"
                     /inference="COORDINATES: protein motif:HMM:NF014543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265103.1"
                     /translation="MRHLREAIGALVLVWMCGTAAQAAPDLSGSDPHWIKDAAKNCWA
                     ANPDPETGETITWTGTCEQGLLSGEGTLSWYVDGQPVGRDEGHFKNGQLSGHGRIVFG
                     DGAHFEGEFPGQGVLTLPSGQEIAAQSVGEAAGWSIEQAQRAPAR"
     gene            5322..6107
                     /locus_tag="VGM72_07290"
     CDS             5322..6107
                     /locus_tag="VGM72_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY4265104.1"
                     /translation="MISVPYKIVWIASMPRSASMWAFNVARALLREAGFSVFPDTLPK
                     SDESMVAEARKGMRDTSPNNVWCLKVHSGLRNASPSNRFISTFRDPRDAVVSFMRFMR
                     YDFDTALSAATIWTQLCDHYRAFPPDRSLCLDYDRIIANPSGVAGRISYFLKLDLAGS
                     EIAQAIAEFEREKIRRRTESLRAEAERGPAAGDVDVAEALILNADGTMRFWDPHTGFQ
                     SDHISDYRDGDWRILLSEDEKRRLDQAIGDWLTQHGYDRRLSA"
     gene            6257..>6823
                     /locus_tag="VGM72_07295"
     CDS             6257..>6823
                     /locus_tag="VGM72_07295"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEY4265105.1"
                     /translation="MTLFRTVPVASGLLMGGLAFALSNGAAQAAQNTQAGKPMQTLQM
                     PAMPEPARVTLNPKTTALLVLDYVEDICNSQPKCKGQMLPAMTPFMERARKAGLTVAY
                     GTRAQNMTKWLPEVAPAAGDIKIVNTGQDRFYNTDLDKALKAKGITTIIMAGWKISGS
                     MTYTAVGAMIRDYTVVIPVDTTTATTDYE"
BASE COUNT         1372 a         2161 c         2020 g         1270 t
ORIGIN      
        1 tcccatgccg cccatgatct ggagcgcctc gttggagact tcccaacccg cgaggttgca
       61 ggcgagcttt gcggcagcgg catcataagc cgacggaaga ccgtctttcg cattggcgat
      121 ggcgcgataa agcagcaatt gcgccgcgtc gagtttgatc gcgctttccg cgaacttcca
      181 ttgcaggccc tggaattcgc acagcaaccg gccgaattgt ctgcgtatca acgcgtaatc
      241 gcgggcagca ttgaacgcat ggcgccccaa cgcgagtgcg cgcgccgcgt tgccgagacg
      301 ttcgacgttg aatccggcga tctgcttttt gaaaccgcct tcgccaagca gcatgttctc
      361 ggcgggaatc cggcaattgt cgaaatacag cggtgcccat tgctcgccgc tcatgaaact
      421 cgagggctcg ccgatcgcaa agccggccgt gccgcgctcc accaggaccg aaccgatgcc
      481 cccgacgccg ggcccatagc gaacatagat caggaacagg ccagcctcgg tgctgtgggt
      541 cgtgaaaatc ttgccgccat tgatgacgaa tgcatcgcca tcgcgtttcg cggacgtgtg
      601 cagatcggtg acggcggacc ccgcatcggg ctcggtcata ccgaggctga tgagctttcg
      661 ccccgccaac agatcgggaa ggaagcgcgc cttctgttcg ggcgatgcat attcggcaaa
      721 ggtacggatc ggaccgaaat ttccggcctg cacgatgtcc gcgcttttcg gacataccag
      781 cgcgacttct tgaatcgcaa tcacggcatc catcagcgtg ccgccgattc cgccgtcctg
      841 ttccgggatt gtgattccca tgaggccctg ctccgccagc agccgggcga tatcccaagg
      901 ataggcgttg ctatgcgcac gcgcgagcgc gccgtccgcc agtttcgcca cagcgaagcg
      961 ccgcacggat tctgcgaaca cgttctgctc atcggatcgt tcaaattcca tcggcccatc
     1021 gcccccgagc gatcaccgcg aaactagctg gccggaagct ccggtgcaaa agctcaatgc
     1081 agatcgacat gcggtaggga gtcgagccgc cacagatgct cgagcaaaat tgccgccttg
     1141 gattcgccaa tgacgggaat cgcgagttcc cgatatttct cctccaactc gtaatccgtc
     1201 agtggcagtt ccggatcgcc tttgcgggtg ggctgcagaa attgttcgcg gcggccatcg
     1261 cgggtttcga tgccaacacg cgccgcgcgg cggccgggaa aaccggcatc gatatcgcca
     1321 tcgcgtttca gcgcgatccg ctccatcaga tcgcacgtca tgggatcggc aatccgctcc
     1381 gcatcgaagg ccgccaatct gacgctgcca tgaacaagcg ccgtcgccac gacataggga
     1441 atgctgaaac gcgcttcacc cggcgtcatc ggccgtggat ttcccgccac ctgcaaggct
     1501 tcgccatagg tctcgacagt tacccatgcg atatcgcggg ctgcgacgcc cagcttcctt
     1561 tgaagctcca gtgcgccgtc aatagccgcg aacgcatgac cgcaacaggc gtggttcttg
     1621 aatgtcacct tcgcgatatg aaactcgtgt ccgagattcg ccagggctgc gttccaatcc
     1681 ggatcgcctc ccatggcgcg gccaaacccc acgtcccctt cgagaacgtc aagagacccg
     1741 gtcacgcctt cgcgcgcggc cagcgccgcg agcagaccgc cttcggcggc acggccggca
     1801 tggaggggct tcgacatgga gccggtacgg aatgcttgct gcagaccggc ggcaaatgtc
     1861 gcggcattgg caagggcgtg ggcaaagcgt tctgggtcca atccgtaaat cactgccgcg
     1921 gcggcggcgg cgccaaaagt gccgatggtt ccggtgttgt gccaaaaccg atagtgcgca
     1981 cgccccatcg ccacgccgat acgcgttgag atttcatagc cggcgatcac ccccttgagg
     2041 aatgtcgcgc cgttcgaccc ctgttcttga gtggctgcga gcacagccgc gattatgggc
     2101 gcgccgggat ggtagatggc ctcacggaag atatcgtcca cttcggctgt atgggccgca
     2161 gtcccgttga tcagggctgc cgcgcgcggg gtggcacggc gccccaacgc tagcttcgag
     2221 gtgccgcggt cgagttcgcc tgcgagcgcg cgctctagaa agatggctgg cgcggaaata
     2281 gcgccgggca acaaggccgc ataccaatcg atgacggcgc gcttggcatg atggacgatt
     2341 tgccccggaa cctgccgttt ctcgaaatcg gcagcgaact cagccaaagt ttgaacgatc
     2401 gtcattccac tctcgctaga agagggcagc tttcgccgga agaagggact tgcgctttca
     2461 cataggacat cgaagccggc aagcacagag ccggaacgaa cattgtccga atcagtaacc
     2521 ggattttgcg tcgcgatttg ttcacgccca atacttgcga tctttcatgg gcattcgagc
     2581 taatccttgt agccccgcgc agtacaagtg ggctgcatta ttaacttccg attaaccgac
     2641 tcaaccaatg ctacaggtgc aatgcgaatc ggcagtggca gtggcgaacg cttcccggac
     2701 gataattgat tgcggcggcg aagatgcaac gcattgcgcc tcaacgcgaa gggatgaacc
     2761 attattggca gacgcggccc ggctcagagc gcgggcacag cgttacgctt ctctggccga
     2821 aacgctgtgc gacccgcaca tggttctggt cgtaagggaa tgtgtccgag aactcgaggc
     2881 agaggcaacg gcgcaagaaa gaatgtccca cgagatctgc gcattggtgc ggtgaccgcg
     2941 cagcatgacg caaatggaaa ctattttccg tcaaaaatgc gcaggcggcc gatccaccac
     3001 cgattgcccg ggaagacctg accgcctagc gcctgccccc tcattgagag gctcgcacac
     3061 cctcgccccc gacagatgcg catggaactt ttgcccgaag cacgacggct tgcaatcgga
     3121 attcgcgtga tcggcgattc ggctgcgtga ttgtggagga aaagccacac tgttgcgcgc
     3181 ccaatcatgg cagacgaaaa tgcttcgaca gtttaagacc ctggcgctgg taattcgaac
     3241 cgaggacttg accgtaaacc tgcggcggcg tgtccgttaa tcgttcgaac acgagccgtc
     3301 cgatggtctg tccatgctcc agcacgaacg gcacctcgcg cgaacgcact tccagcaccg
     3361 cgcgcgcacc gcgcccatgc ccgctttcgt gcccgaagcc cggatcgaag aaaccggcat
     3421 agtgcacgcg aaactcgccg accagcggat tgaacggcat catctcggct gcataatcgg
     3481 gcggaatgga aatcgcctcc cgcgacgcga gaatatagaa ctcgtccggg tccaggataa
     3541 tgctggtgcc tttgcgtgca tagatcggat cccagaaatc gagcggatcg tgggcgccgc
     3601 gcacgtcgat atcgatgacg cccgcatggc gccgcgcgcg ccagccgact tggcgcgtca
     3661 gggtttcgcc gcgaagatcg accgagagcg caagtccgcc ttcgatatcg gcgggcccat
     3721 cgaccaagcc cacctcctca tgaaggcgct ttaattgggt gtcgctgaat tgcggcgagc
     3781 catggcgcaa ccgcagctga ttcagccgcg atcccttgcg gaccagaagg gggaaggtgc
     3841 gcggtgatat ctcggcataa agcgggccat gatagccctc ctcgacccgg tcgaaggctt
     3901 gcgcgtgatc ggtgatcagc cgcgtgaaga catccagccg cccggtggag cttttcggat
     3961 tcgccacgcc cgacacccgg aacgacagct ccagccgctc cagcagaggc acgaggtaga
     4021 cgcaaccggt ctccagcacc gcacccggtc tcagatcgat ttcgtggatc gccagttcgg
     4081 acagtttgtc ggcgatgcgc gcatcccgtc cgggcaggaa gcttgcccgg atgcgatagg
     4141 cattggcccc gagcctcaga tccaggctgg ccggctggat ttgctcctca atgacctcct
     4201 ccgccgccag cacttcgcgc cggccgcgga tcagacggcg cagcacatga gagggaagga
     4261 tccccgtcga atatttgcca tagggattgt ccccgccgcc cctctcgcca gcgagctcat
     4321 cgaagggaaa gccggtcgga tcactcacat tcttgcccat gccgtgtgat tagccggagt
     4381 cgcacgcgcg gagtagcggg gcgaaactcc tatccaaaga tatttcatcc cgccataccc
     4441 gaacggacgc tcagcgcgcg ggggcccgct gggcctgctc gatgctccag ccggcggctt
     4501 ccccgacgga ttgcgccgct atttcctgcc cgctcggcag cgtgagcacg ccctggccgg
     4561 ggaattcgcc ttcgaagtgc gcgccatcgc cgaaaacgat gcggccgtgc cccgaaagct
     4621 ggccattctt gaaatggcct tcgtcgcgtc cgaccggctg gccatcgaca taccaggaga
     4681 gcgtgccctc tccgctcagc aggccctgtt cgcaggtccc cgtccaggtg atggtctcac
     4741 cggtttccgg gtccggattg gcggcccagc aattcttcgc cgcgtccttg atccaatgcg
     4801 ggtcgctccc ggaaagatcg ggagccgctt gcgcagccgt gccgcacatc caaaccagga
     4861 cgagagcgcc gatcgcttca cgcaaatgtc tcatcggcaa acctcgtgca aggaaagcgg
     4921 cagcatcgaa acgcgagtat ggcactgggc agccggggtc ccaaacgcgc aggccccctc
     4981 ccccctcaaa actgccggcc ggccgcaaat ttctgcgcgc tttgcttggg ttcttggccg
     5041 aaccggacgt gattttttcg gtgcaatagc ggtgcaattg ggtgcatttg ccggccgcat
     5101 gatttcctcg aagaatttct agcccgaagt atacggccat ccggcgatgg aggggataag
     5161 tcaaatgcct cggcatcgga tagtgtcagg cgccgtgcgt ccggtttcgt acattaaggc
     5221 ggcccgactt ttcacagaat gtgccgaagc cacgttcggg caaagctgtg aggcatttat
     5281 ctcttagcgt ttgggttcgc atcggccggc gggcactcgc catgatttct gttccataca
     5341 agatcgtgtg gatcgcgagc atgccgcgct cggcttcgat gtgggccttc aatgtcgcgc
     5401 gcgcgctcct gcgcgaggcc ggcttcagcg tctttcccga cacccttccc aaaagcgacg
     5461 aaagcatggt ggccgaggcg cgcaagggaa tgagagacac ctcgccgaac aatgtctggt
     5521 gcctgaaggt ccacagcggc ttgcgcaatg cttcgccatc caaccgcttc atcagtacgt
     5581 tccgcgatcc acgcgatgcc gtcgtgtcgt tcatgcgctt catgcgctat gatttcgaca
     5641 ccgctctttc ggcagccacc atctggaccc aactctgcga tcattaccgg gcgtttccac
     5701 cggaccggtc gctgtgtctc gactatgaca ggatcatcgc caatccttca ggcgtcgcgg
     5761 gacggatcag ctattttctc aagctcgatt tggcggggag cgaaatcgcc caggcaatcg
     5821 cggagttcga acgcgaaaag atacgccggc gtacggaatc cttgcgcgcc gaagcggagc
     5881 ggggaccggc ggcgggcgat gtggatgtcg ccgaagccct gatcctgaac gcggacggca
     5941 ccatgcgttt ctgggatccg cacaccggct ttcaatccga tcacatctcg gattaccgcg
     6001 acggcgattg gcgcatcctg ttgagcgaag acgagaaacg gcgcctcgat caggccatcg
     6061 gcgattggct gacacagcat ggctatgacc ggcgtctcag cgcgtaaggc gggttatggg
     6121 tgaaggcggt tcaattccat cgggccttgc tttagtgtag gtcttggccc cgcttcttcc
     6181 gaagctcgca agcgtgcatt ctcaaatccc tgcgaatttc gcaaaaatcg caaacctgtt
     6241 cctggagatt gccaatatga cgctgtttcg cactgtcccc gtggcgagtg gcctcctgat
     6301 gggtggcctc gccttcgcgc tctcaaacgg tgcggctcaa gcggcccaaa acacgcaggc
     6361 cggcaagcct atgcagaccc tacaaatgcc ggccatgccg gaaccggccc gcgttacgct
     6421 gaaccccaaa accacagctt tactggtgct cgactatgtc gaggacatct gcaactccca
     6481 gccgaaatgc aagggccaga tgttgcccgc catgacccca ttcatggagc gggcccgcaa
     6541 agccggcttg accgtggcat acggcacccg cgcgcagaac atgaccaaat ggctgcccga
     6601 ggtcgcgccg gccgcgggcg acatcaagat cgtgaacacg ggccaggaca gattctacaa
     6661 caccgatctc gacaaggcgc tgaaggccaa gggcatcacg acgatcatca tggccggctg
     6721 gaaaatcagc ggctcgatga cctacaccgc ggtcggcgcg atgatccgcg attacaccgt
     6781 cgtcatcccg gtggatacga ccacggccac caccgactat gag
//
LOCUS       DASYEG010000160         7063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_773790, whole genome shotgun sequence.
ACCESSION   DASYEG010000160 DASYEG010000000
VERSION     DASYEG010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 7063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7063
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773790"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="VGM72_07300"
     CDS             <1..1054
                     /locus_tag="VGM72_07300"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY4265106.1"
                     /translation="ITNHVGGTITSDQRAITVDDSNLGNAFAAVTISNEGTISGANGE
                     AISITSTFANTLTNKGTINGTVAMGAGADTVNLYTGSSSGAIDGGAGADTLHLAGTGK
                     GTLGAVSNIETLKVDAGHWVVSNAQSYTSGGAIAVGATLQVDHTTASFGGTFIVSGGL
                     TSDPATLKFNDLSVDPTGYINAEGGDLYQLASSFLNHSTQNALWDTTTALLEFTGLAG
                     TSHDLLLTGADRGGFWGGYAQNFAWGELSIDDGNELVLDSGAPGGGAFYVGSLIGVLF
                     TGDSVTNIVGNGFNIYYDPSAAGNAYLGGGTYRLLDGGVLMAAVPEPRSIALVLSGLG
                     VLALRRRRRKSKAIAS"
     gene            complement(1049..1477)
                     /locus_tag="VGM72_07305"
     CDS             complement(1049..1477)
                     /locus_tag="VGM72_07305"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEY4265107.1"
                     /translation="MSDPLYAKDLLRLAANATGAGRLLPNSAEGHAHNPTCGDRISVT
                     LRLDDAGRIAAMAHETQACVLAQASASILGSHLAGADASAVRTLRAQVTAMLREGAVP
                     PIPFADYSTLTGAARFRNRHSCVLLPIDAVLDALSKADAS"
     gene            complement(1474..3090)
                     /locus_tag="VGM72_07310"
     CDS             complement(1474..3090)
                     /locus_tag="VGM72_07310"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding/glycosyltransferase family
                     2 protein"
                     /protein_id="HEY4265108.1"
                     /translation="MRFGTFPLDEAEGTILVHSLRVGGRLFKKGRVLAKPDLEALGQE
                     GIVSVMAARLEPGDVPEDVAATSVARAFAGAGTRLSAAFTGRTNLYAEQSGIVMIDAE
                     AIDAVNLIDEAVTVATLAPYAVVAPGEMLATIKIIPYAAPQWAVAAAVAAARATSIRV
                     AQFKPMRVALISTQLPGQKPILLDKNRTALEARLMPLGGRLIFERRIPHDAQSVSDAL
                     CAAEAAGAELLFVFGASAITDRRDVIPAGIIAADGTVLHFGMPVDPGNLLLLGDLRGR
                     TVIGLPSCARSPKVNGFDFVLQRLFAGLTLTSSDIMRMGVGGLLQEISTRPQPRDAER
                     PALRAPRIAGLVLAAGLSSRMGSNKLLQEWRGKPLLRWTVEAALNSDARPVIVVTGND
                     AQKIEAALKGLDVRFAHNRDFRDGLSASLKTGIAAVPDTADGVLVLLGDMPEVDSNLM
                     NRMVAAFSPADNRAICIAVHDHRRGNPVLWSRAFFPEIAALSGDEGAKRVLSKHEDVV
                     CEIEAAGAVLRDIDTPEALAALRAADSATV"
     gene            complement(3100..4494)
                     /locus_tag="VGM72_07315"
     CDS             complement(3100..4494)
                     /locus_tag="VGM72_07315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4265109.1"
                     /translation="MRILTTLRSQFVAVVTTAVILSNLAVVAILEIGREGELQSARVN
                     AAVDRIAAVFRYIITIPPSQQDSAVATLSGNLFHYSITQTPPFTAPRMSEEERQMAAD
                     LLAGERLSNMGPALVRLTEQSGTPLNNDGAAFEITQPLPSGDGWVTAQFARPPTPSPA
                     PPILIAAALGTIITGAAAAWLAGRVSRPLSALAFAADAVARGRTAPRLAANGPDDIRR
                     ASEAFNEMSGRVTRTLESQRQLLSAVGHDLRTPLAAMRITAEFVQDREVQDRLTRNLD
                     ELQSMTEAVLSAARAGPGEEERRVDLATLIESVCDDLIELNQPVEVSIHGLAPCSCRS
                     NEIRRAARNLIENAVRYGGTARVTLRTEPDFFVATIEDDGPGIPADRLEEVFEPFVRL
                     EESRNAATGGSGLGLTLARSIAREHGGDVILENRKSGGRIVGLRASLRLPREKTRGKR
                     EFLTGAIAPAAAST"
     gene            complement(4494..5222)
                     /locus_tag="VGM72_07320"
     CDS             complement(4494..5222)
                     /locus_tag="VGM72_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4265110.1"
                     /translation="MPESGVQPHILVVDDARDIREPLVRYLKESGYRASAADGAQAAR
                     RLMRTSGIDLVILDIMMPGEDGLSLCRSIRENSGIPVIMLTARGEELDRIVGLEVGAD
                     DYVAKPFNPRELLARVGAVLRRTNTLPPRLRGPTAERYRFGEWVFNAAQREIVGPEGL
                     ALPLSSGEFKLLMAFLERPKVALSREQLLDLTKGRDAEVFDRSIDNQVSRLRRKIELD
                     PKNPRYIKTVWGGGYMFTSDVEIL"
     gene            complement(5454..6467)
                     /locus_tag="VGM72_07325"
     CDS             complement(5454..6467)
                     /locus_tag="VGM72_07325"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HEY4265111.1"
                     /translation="MDIPTPDLADPLAAATAWMHGQHRVALATVTRTWGSAPRPGGSQ
                     MAVREDGAFTGSVSGGCVEGAVIGEAQNAIRDGKIRNLKFGVSDSDAWAVGLACGGTI
                     EVHVAPVIAASQQAVIAELKHARDAQRAAVLAIDLSGGESRVLFPGGDAHDPLTAAAR
                     LQARRDQSAAVEIEGRSWFLTVFNTPLDLAIVGAVHIAQPLSRMATLADYAVRVIDPR
                     TAFANAERFPGLTLVHEWPDEALTAQPLTARSAIVALTHDPKFDDPALAAALKSPAFY
                     IGALGSRTTHARRLDRLRALGFADSDLARIHGPIGLDLGARSPAEIAVAILGQMTAIL
                     RKG"
     gene            complement(6476..>7063)
                     /locus_tag="VGM72_07330"
     CDS             complement(6476..>7063)
                     /locus_tag="VGM72_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEY4265112.1"
                     /translation="AGTPHADVAASEVVRHAIPALAVLAGEIGDPAVRHKGTLGGSVA
                     NNDPAADYPAAVLGLGAQLRTTSRNIDGDGFFTGMFGTVLEPGEIITEISFPLPRRAG
                     YEKFRNPASRYAMVGVFVADFPGSVRVAITGAAPCVFRFGKMEAALNKDFTPAAIADI
                     AMAPEGLNSDLSGTAEYRAHLVGVMAKRAIETAIR"
BASE COUNT         1341 a         2234 c         2244 g         1244 t
ORIGIN      
        1 catcaccaat catgttggcg gcacgatcac gagcgatcaa cgcgcgatca ccgtagacga
       61 ctcaaatctc ggcaatgcgt tcgcggccgt cacgatcagc aatgagggca ccataagcgg
      121 ggccaacgga gaggcgatca gtatcacgag tacctttgcc aacacgctca ccaacaaagg
      181 caccataaac ggcaccgttg ccatgggtgc tggcgccgat acggtcaatc tctacacagg
      241 ctcgtcgagc ggtgcgatcg acggcggtgc aggcgccgac acgcttcatc ttgcgggaac
      301 cggcaagggc acgctcggcg cggtgagcaa tatcgaaacg cttaaagtcg atgccggcca
      361 ttgggtggta agcaatgcgc aaagttacac atcgggcggc gccatcgccg tcggtgcgac
      421 gcttcaggtg gatcatacga cggccagttt tgggggcacg ttcatcgtca gcggcggttt
      481 gaccagcgat cccgcgaccc tcaaattcaa cgatctatcg gtcgacccaa ccggatacat
      541 caacgccgaa gggggcgatc tctatcaact cgccagcagt tttctcaacc acagcaccca
      601 gaatgcgctg tgggatacca caacggcact cttggagttc accgggctcg ccggaacgag
      661 ccatgatctt ctcctcacgg gtgccgatcg cggcgggttc tggggcggat atgcgcagaa
      721 tttcgcctgg ggcgaattgt cgatcgatga cggcaacgaa cttgtcctcg acagcggcgc
      781 gccaggcggc ggcgcgttct atgtcgggtc tctgatcggt gtgttgttta caggagacag
      841 cgtcacgaac atcgtcggca atggcttcaa catctattac gatccatccg cggcgggcaa
      901 tgcgtatctc ggcggtggca cctaccgctt gctcgacggt ggcgtcctga tggccgcggt
      961 gcccgagccg aggagcattg cactcgttct ttctggtctg ggtgttctgg ccctcaggcg
     1021 gcggcgccgc aaaagcaagg cgatcgcgtc atgatgcgtc cgctttcgaa agagcatcca
     1081 gcaccgcatc gatcggaagc agcacgcagg aatggcgatt gcgaaaacgc gccgctccgg
     1141 tgagcgtcga atagtccgcg aacgggatgg gcggcacggc gccctcgcgc agcatcgccg
     1201 tcacctgcgc gcgcaaagtc cgcactgcac tggcatcggc gcccgcgaga tgcgacccca
     1261 ggatcgatgc ggaggcttgc gccaacacac aggcctgcgt ctcatgtgcc atggcggcga
     1321 tgcgtcccgc atcgtccaag cgaagcgtca ccgagatgcg gtcgccgcag gtcggattat
     1381 gcgcgtgacc ctccgcgctg ttgggaagca ggcgcccggc gccggttgca ttcgccgcca
     1441 gccgcagcag gtccttggcg taaagcggat cgctcatacc gtggcggaat ccgccgcccg
     1501 caaagccgca agcgcttcgg gcgtgtcgat gtcccgcagc acggccccgg ccgcttcgat
     1561 ctcgcagacc acgtcctcgt gcttcgacag cacgcgcttg gcgccttcat cgccgctgag
     1621 cgccgcaatc tcgggaaaga aagcgcgcga ccacaggacc gggttgccgc gtctatgatc
     1681 atgaacggcg atgcaaatgg cacgattgtc cgcgggcgag aacgccgcga ccatgcggtt
     1741 catgagattc gaatccactt cgggcatgtc gcccagaagc acgagtacgc cgtcggccgt
     1801 gtccggcacc gcggcaatcc ccgtcttgag cgaggcgctg agcccatcgc ggaaatcgcg
     1861 attgtgcgcg aagcggacgt cgagcccttt gagtgcggcc tcgattttct gcgcatcgtt
     1921 cccggtgacg acaatgacgg ggcgcgcatc gctgttcagc gcagcctcta ccgtccagcg
     1981 taacagcggc ttgccacgcc attcctggag caatttgttc gatcccatcc gcgaggacaa
     2041 tcccgccgcg agcacaagtc ccgcgatgcg cggtgcccgc aatgcgggac gctcggcatc
     2101 gcgcggttgc gggcgggtgg atatttcttg cagcagtccg ccgacgccca tgcgcatgat
     2161 gtcgctgctc gtgagcgtga gacccgcaaa aagacgctgc aggacgaaat cgaagccatt
     2221 gaccttgggc gaacgcgcac agctcggaag gccaatcacc gtgcggccac gcaaatcgcc
     2281 gaggagcagc aaattgcccg gatcgactgg catgccgaaa tgcaaaaccg tgccgtccgc
     2341 ggcgataatc ccggcgggta tcacatcgcg tcggtccgtg atggccgagg caccgaaaac
     2401 aaaaagcagc tctgcccccg cggcttcggc cgcgcacaat gcgtccgaca cggattgcgc
     2461 gtcatgagga atgcgccgct cgaagatcag acggcctcct aaaggcatca agcgcgcctc
     2521 cagcgcggtt ctgttcttgt cgagcaatat tggcttttga ccgggcagtt gggtcgaaat
     2581 cagcgccacg cgcatcggtt tgaattgcgc cacgcggatc gatgtggcgc gcgcggctgc
     2641 cacggcggcg gcgacagccc attgcggcgc tgcatacgga atgatcttga tcgtggcaag
     2701 catctcgccg ggtgcaacca ccgcataagg ggcaagggtc gccaccgtga cggcttcatc
     2761 gatcaaattg accgcatcga tggcttcggc atcgatcatc acgattcccg attgctccgc
     2821 ataaagattt gtccgcccgg taaaagcggc gctaagcctc gtgccggcgc cggcgaaggc
     2881 ccgtgcgacg ctggtcgcgg cgacatcttc gggcacgtct cccggttcga gccgggctgc
     2941 catcaccgat acgattccct cttggccaag tgcctccaga tcgggtttcg cgagcacgcg
     3001 gcctttcttg aagaggcggc cgcctacgcg cagggagtgc acgagaatag tgccttcggc
     3061 ttcgtcaagc ggaaatgtcc cgaaccgcat caacgcaaat taagttgagg cggccgccgg
     3121 agcaatcgcg ccggtgagaa attcgcgttt gccgcgggtc ttctcgcggg gaagccgcag
     3181 agaagcgcgc aggcccacga tgcggccgcc gcttttgcgg ttctccagga tgacatcgcc
     3241 gccatgctcg cgcgcgatcg agcgggccaa cgtcaacccc agccccgatc cgccggttgc
     3301 ggcattgcgc gattcctcaa gacgaacgaa aggttcgaag acttcctcga gccggtcggc
     3361 ggggataccg gggccgtcat cctcgatcgt cgcgacaaag aagtccggtt cggttctcag
     3421 cgtcaccctg gccgtgccgc catagcgcac ggcattctcg atcagattgc gggcggcgcg
     3481 gcgaatttcg ttggaacggc aactgcacgg agccagacca tggatgctga cctccacggg
     3541 ctgattcagt tcgatgagat cgtcgcacac gctttcgatc aaagttgcga ggtcgacccg
     3601 gcgctcttct tcgcccggtc ctgcgcgcgc tgccgacagc acggcctcgg tcatcgattg
     3661 aagctcgtcg agattgcgcg tcaggcgatc ctgcacctca cggtcctgca caaattcggc
     3721 ggtgatgcgc atggcggcta acggcgtgcg cagatcgtga ccgacagcgg aaagaagctg
     3781 gcgctggctt tcaagcgtgc gggtaacgcg tccgctcatc tcgttgaagg cttcggaggc
     3841 ccggcggata tcgtccggac cgttggcggc cagccggggc gcggtgcgtc cgcgcgccac
     3901 ggcatcggcg gcaaacgcca gggcagacaa aggccgcgag acgcgtccgg caagccaggc
     3961 cgcggctgcg ccggtgatga tcgtgcccaa agcggcggcg atcaaaatcg ggggagcagg
     4021 ggagggcgtg ggaggacgcg cgaactgtgc ggttacccag ccgtccccgg aaggaagcgg
     4081 ctgcgtgatc tcgaaagcgg ctccatcgtt gttgagcggg gtcccgcttt gctcggtgag
     4141 acgcacgagc gccggcccca tattgctgag gcgctctccg gcgaggaggt cagccgccat
     4201 ttgccgttct tcctcgctca tgcgcggggc ggtgaatggc ggggtttgcg tgatggaata
     4261 gtggaacaga tttccgctga gcgtcgcgac ggcggaatcc tgctgcgacg gcggaatcgt
     4321 gatgatgtag cggaacacgg ccgcgatgcg atcgaccgcg gcattgacgc gcgcggactg
     4381 caattcgcct tcgcggccga tctccaggat cgccaccacg gcaagattgg acaggatcac
     4441 cgcagtggtc accaccgcga cgaattgcga ccgcagcgtt gtcaaaatcc gcatcagagg
     4501 atctccacat cggaggtgaa catatagccg ccgccccata cggttttaat atagcgcgga
     4561 ttcttgggat ccagttcgat cttccgccgc aggcggctga cctgattgtc gatcgaacgg
     4621 tcgaacacct ccgcgtcgcg acccttggtg agatcgagca attgctcgcg cgagagcgcg
     4681 actttcggcc gttccaggaa cgccatcagc agcttgaact cgccactgga aagaggaagc
     4741 gccaagcctt cggggcctac gatctcacgt tgggcggcgt taaagaccca ttcgccgaaa
     4801 cgatagcgct cggctgtggg cccgcgaagc cgcgggggaa gcgtgttggt ccggcgcagc
     4861 accgcgccca cgcgtgccag caactcacgg gggttgaagg gtttggccac gtaatcgtcc
     4921 gcgcccactt cgaggccgac gatgcggtcc agttcttcgc cgcgcgccgt cagcatgatc
     4981 accggaatgc cggaattttc ccggatggaa cggcaaagcg ataacccgtc ctcaccgggc
     5041 atcatgatgt ccagaatcac gagatcgatg cctgaggtcc gcatgagccg ccgcgcggct
     5101 tgcgcgccat cggcggccga agcgcgataa ccactttcct tgaggtaacg caccagcggt
     5161 tcccgaatgt cccgcgcgtc atcgaccacc aggatatggg gttggacgcc gctttccggc
     5221 aaagattttg tcctttggtt gccgcttaac atcctcaacc gtccagggct cgcaagggtg
     5281 aacctgtcgc aaaatatgtc atctcgcggg aaacggcaaa gagcggtgcg ctttacccaa
     5341 ttttcgagat agctctggca ccgtcttgag gtctcctagg tgcgctaacg ggcacgacat
     5401 tgcgtgaact ttagagccgg gcggccccct atcccaatcc gcccggcctt tttttatcct
     5461 tttcgcagaa tcgccgtcat ttggcccaaa atcgccactg cgatctcggc cggcgagcgc
     5521 gcgccgagat cgagcccaat ggggccgtgg atgcgcgcca gatcgctgtc ggcaaagcct
     5581 agcgctctca gccggtccag gcgccgcgca tgggtcgtgc gcgaaccgag agcgccgata
     5641 tagaaggccg gcgatttcaa ggccgcggcg agcgcgggat cgtcgaattt cggatcgtgg
     5701 gtgagggcga cgatggcgct gcgcgccgtc agcggctgtg cggtgagcgc ctcgtcgggc
     5761 cattcatgga caagcgtgag cccgggaaag cgttccgcgt tcgcaaaggc cgtgcgtgga
     5821 tcgatgacgc gaacggcata atcggcaagc gtcgccatgc gcgagagggg ctgtgcaata
     5881 tgcacggcgc cgacgatcgc caggtcgagc ggcgtgttga acacggtcag aaaccaggac
     5941 cgcccttcga tctccacggc tgcgctctga tcgcggcgcg cctgcaggcg tgccgcggcg
     6001 gtcagtggat catgcgcgtc gccaccggga aaaagcacgc ggctttctcc gccggaaagg
     6061 tcgatcgcga gcaccgctgc acgttgagca tcgcgggcgt gttttagctc tgcgatgacg
     6121 gcctgctgcg aagcggcgat gaccggggcc acatgaactt cgatggttcc gccgcaggcc
     6181 agcccgaccg cccaggcgtc cgaatcgctg acgccgaatt tcagattgcg gattttgccg
     6241 tcgcgaattg cattttgcgc ttcgccgatg acggcgcctt cgacgcaacc gccggataca
     6301 gagcccgtga acgcgccatc ctcgcgcacg gccatctggc ttcctccggg ccgcggcgcc
     6361 gagccccagg tgcgcgtgac ggtcgccagc gcaaccctgt gctggccatg catccaagcc
     6421 gtagcggctg ccaagggatc ggccaagtcc ggagtgggaa tatccatgct tgccattatc
     6481 gtattgccgt ttcgatggcg cgcttggcca tgacgccgac cagatgggcg cgatattcgg
     6541 ccgtgccgga aagatccgaa ttgagtcctt cgggcgccat agcgatgtcc gcaatcgcgg
     6601 cgggcgtaaa atctttgttc agcgcggctt ccattttgcc aaagcggaac acacagggtg
     6661 cggcgccggt aatcgccacc cgcacgctgc cgggaaaatc cgccacgaag acgccaacca
     6721 tggcatagcg cgatgcagga ttgcggaatt tctcatagcc cgcgcgccgc ggcaggggaa
     6781 acgaaatctc tgtgatgatc tcgcccggtt ccaagaccgt cccgaacatc ccggtgaaaa
     6841 atccatctcc gtcgatgttg cgcgacgtgg tccgcagctg tgcgcccagg ccaagcacgg
     6901 cggctggata atccgcggcc ggatcattgt tcgccaccga tcctcccagc gttcccttgt
     6961 gccgcacggc gggatcgccg atctcaccgg ccaagaccgc gagagccggg atcgcatgtc
     7021 tcaccacctc ggacgcagcc acatcggcat gcggcgtacc ggc
//
LOCUS       DASYEG010000161         5669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4108101, whole genome shotgun sequence.
ACCESSION   DASYEG010000161 DASYEG010000000
VERSION     DASYEG010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5669
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4108101"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VGM72_07335"
     CDS             complement(<1..369)
                     /locus_tag="VGM72_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265113.1"
                     /translation="MDMRRGNFLLFALAAMAAAPALGRDAAPTVGAASTASQSAASVP
                     DFSRLWANTLGPGFPLPASGPGPVLNKARQRAAVDIYGRDIDPAKAPIVSAPGRRVGD
                     DAAPILQPWAAQVVKQHAEKE"
     gene            complement(527..602)
                     /locus_tag="VGM72_07340"
     tRNA            complement(527..602)
                     /locus_tag="VGM72_07340"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(567..569),aa:Phe,seq:gaa)
     gene            complement(702..917)
                     /gene="yacG"
                     /locus_tag="VGM72_07345"
     CDS             complement(702..917)
                     /gene="yacG"
                     /locus_tag="VGM72_07345"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEY4265114.1"
                     /translation="MTQSRTCPICKRPLDTGDEGERVRPFCSKRCADIDLGHWLMGSY
                     TIPAVESEGETEDEDGGNEGDSGILRH"
     gene            complement(935..2482)
                     /locus_tag="VGM72_07350"
     CDS             complement(935..2482)
                     /locus_tag="VGM72_07350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEY4265115.1"
                     /translation="MPREVLINAGAGEVRVAVIEDGLLDQLWLERTIGFEEIPGRKRE
                     RAANGSSRSIVGDIILGRVQRVLPAMQAAFVNIGLDRAGFLAAREARPRTAIFRDDPY
                     TEEDRQLHISDFAREGDEILVQVVKDPIAEKGARLSANITIAGRLLILVANSPGVALS
                     RRIDAEAERARLTSIISSFAADTTGDLIPEAGYILRTAAIGAGEAEIRDDAVRLCAAW
                     REIAARKKAAVSPATLYMDIGPIERALRDEVDGSVGRVLIDERDAAAAARAYAQLAMP
                     EMSGKIQLYEGRLPLFEAFGIEDEIVTLSHPRVSLPSGGWITVEATEALTAIDVNSGS
                     YTASGGLEETSLKVNLEAAEAIGRQLRLRGIGGLIVIDFIHLAEPANIQRVLDALTLA
                     CAKGRVPSQILGMSEFGLVEMTRKRLRDPLAKRLTEDCRRCDGHGRRKTVETVAVEMM
                     RRMERSAAAAPGKEIVLRAAPVMVRWLEAHTEEIRGALTRRGVLRYRIEAHSDSRREN
                     FDVETAV"
     gene            2662..3765
                     /locus_tag="VGM72_07355"
     CDS             2662..3765
                     /locus_tag="VGM72_07355"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265116.1"
                     /translation="MPMSNRSTHLAVGLIAGLGLGFLPHPAAAEGPVFKVDPSWPKTL
                     PNNWILGQIGGLTVDGQDHIWIDQRPRSLTKDEAGAAQKPPQSKCCMPAPPIMEFDAA
                     GNFLKGWGGPGEGYDWPEQEHAVRLDGKGNIYLAGNGAKDQMLLKFTTEGKFVKQLGK
                     LSMPLGSKDTDSVGRVADIFYDAPANELYLADGYGNHRVMVIDADSFAFKRMWGAYGK
                     PPSDDNIPMYNPASAQFANPVHCVKIASDGLVYVCDRSNNRIQVFQKNGTFVKEFVYD
                     KDTRQSGSTWDIALWPDKNNTYLLMADGTNNLIRVIRRADGQVLSTFGTGGRQAGQFH
                     WVHVMAIDSKGNLYTGEVDTGKRIQKFTSNMTP"
     gene            3828..4913
                     /locus_tag="VGM72_07360"
     CDS             3828..4913
                     /locus_tag="VGM72_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265117.1"
                     /translation="MKNRLKLILALVLGALMSSSSAFAEGPEYKVDASWPKQLPNNWI
                     IGQIGGMNVDAQDHIWVFQRPRSLKPVDLAETKHAKCCIAAPSVIEFDAAGNVLSSWG
                     GPGYNPDWPEVEHGIFVDGKNNILLTGSGNKDGTLLKFSRDGKFLAKFGHQGPTAGSQ
                     DTSALGGTASIAFDPQANELFLSDGYFNHRVIVLDADSLAYKRMWGAYGKPPADMKLP
                     NYNPKSAQFANPVHCVRIANDGLVYVCDRTNDRIQVFQKNGTFVKEFAIRPETHGTGS
                     SYDLAFWPDKNQTYLIIADGADGEAVIVRREDGKEVGAFGHYGKQAGQFHNIHQIVSD
                     SKGNIYTGEVDVGMRVQKFVPNMPPAK"
     gene            complement(4946..5596)
                     /locus_tag="VGM72_07365"
     CDS             complement(4946..5596)
                     /locus_tag="VGM72_07365"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HEY4265118.1"
                     /translation="MAATRASSLPLVLASASPRRAALLAQAGIQLARIVAPDVDETPK
                     KQELPRVYCLRLALAKAAAVAKILSLPPLEAGADGEAVRGGEFVILAADTVVVCGRRI
                     LPKAEDAAEVERCLRVLSGRRHQVLTALALIAPNAKPRTRVVTTRVAFKVLTPREVAE
                     YGASREGEGKAGGYAIQGRAEALVKSINGSYSNVVGLPLYETMSLLGGERRIANSE"
BASE COUNT         1154 a         1798 c         1649 g         1068 t
ORIGIN      
        1 ctctttttcg gcgtgctgct tcacgacttg cgccgcccag ggctgcaaaa tcggagcggc
       61 gtcatcgcca acccggcggc cgggcgcgct cacgatggga gccttcgcgg gatcgatgtc
      121 ccggccgtag atatcgaccg cagccctctg gcgcgccttg ttcaatacgg gacccggtcc
      181 cgaagccggc agcgggaagc cggggccgag cgtattggcc cacagccgcg agaaatccgg
      241 gacagatgcg gcgctttgcg atgcggtgct cgctgcccct acggtcggcg ctgcatctcg
      301 ccccaaggca ggtgctgccg ccattgccgc caacgcaaat aaaaggaaat tcccgcgtcg
      361 catatccatg cgaatctccc tttatgtttg ttccggaccg accgacttgt agcctgcttt
      421 cccgcccgcg agaaagcgaa cgaaccgagc gcaagagaat ggacaaacca gcggacaaac
      481 tgtccgttgt ttcttctgag aaaaccgcta agccattgaa atcaaatggt gcccaggggc
      541 ggaattgaac caccgacacg cggattttca gtccgctgct ctaccaactg agctacctgg
      601 gcgccaagcc atcggaaaaa cggcccggtt ttgtagaaga cactccatcc cctgtccagg
      661 gaagcctcgc ggattttcgc agcgatgctc ccgcagatgg tttaatggcg caaaatgccg
      721 ctgtccccct cattgccgcc gtcttcgtct tcggtctctc cctcgctctc cacggccggg
      781 atcgtatagc tgcccattag ccaatggccc aaatcgatgt cggcgcagcg cttggagcag
      841 aaagggcgaa cccgttcgcc ttcatccccc gtgtctagcg gccgcttgca gatgggacag
      901 gtccgcgatt gggtcatgca ggagaacgcc tcgttcagac agcggtttcc acatcgaaat
      961 tctcgcgccg cgaatcgcta tgcgcctcga tgcgatagcg caggaccccc cggcgtgtca
     1021 gtgcgccgcg gatttcctcc gtatgggctt cgagccagcg caccatcacc ggggcagcgc
     1081 gcagcacgat ctcctttcca ggcgcggcgg ccgcgctgcg ctccatgcgg cgcatcatct
     1141 cgaccgcgac cgtttccacg gttttgcgcc gcccgtgacc gtcacaacgc cggcaatcct
     1201 cggtcagccg cttggccaat ggatcgcgta ggcgcttgcg cgtcatttcg accaagccga
     1261 attccgacat gcccaggatc tggcttggca cccggccctt ggcacaagcc agagtcaatg
     1321 catccagcac acgctgaatg ttcgcgggct ccgccagatg gatgaaatcg atcacgatca
     1381 acccacctat cccacgcagg cgcaattgcc ggccgatcgc ttcggccgct tcgagattca
     1441 ccttgaggct ggtctcttcc aacccgccgg atgcggtata gcttccggaa ttgacgtcga
     1501 tggcagtgag cgcctctgtc gcttcgacgg tgatccagcc acccgagggc aatgacaccc
     1561 gcggatgaga cagcgtaacg atctcgtctt cgatgccgaa ggcttcgaag agcggcagac
     1621 ggccttcata caattggatc ttgccgctca tttcgggcat cgcgagttgg gcataggcgc
     1681 gcgcggccgc agcggcatcg cgctcatcga tgaggacacg cccaacgctg ccgtccacct
     1741 catcgcgcaa ggcgcgttcg atgggaccaa tatccatata aagcgtggcg gggctgaccg
     1801 cggctttctt gcgcgccgcg atttcgcgcc aggccgcaca gagccgcacc gcatcgtcgc
     1861 gaatctccgc ttccccggct ccgatcgcgg ccgtgcgcag gatgtagccc gcttccggga
     1921 tgagatcgcc cgtggtatcg gccgcaaagc tggaaataat ggaggtcaac cgcgcgcgct
     1981 ccgcttcggc gtcgatgcgc cgcgacagtg ccacgccagg cgaattggca accaggatca
     2041 aaagccggcc cgcaatggtg atattggccg aaagtctcgc gcccttctcg gcgatcgggt
     2101 ctttgacgac ctggacaaga atttcatcgc cctcgcgcgc gaaatccgag atgtgaagct
     2161 gccggtcttc ttccgtatac ggatcgtcgc ggaagatcgc cgtgcgcggg cgcgcttcgc
     2221 gggcggccag aaagccggcc ctgtcgaggc ctatattgac aaaagcggcc tgcatggccg
     2281 gcagaacgcg ctgcacccgg ccgaggataa tgtcgcccac aatgctgcgc gaagacccgt
     2341 tcgccgcgcg ctcgcgtttc ctgccgggaa tctcctcgaa accgatggtg cgttcgagcc
     2401 aaagctgatc gagaagacca tcctcgatga ccgcgactcg cacttcgccc gctccggcgt
     2461 tgatcaagac ttctctgggc attggaactc cgtttgagcc aatctagcag gggccgacga
     2521 gaattgcttt catttgcgcg agttccattt gaaactacac gtcgcgcatg tctaatatcg
     2581 gcagaaccgt gcgttaaaat ggctgtcacg aggcggcgca agcattgtct ctcgcttttt
     2641 ctgaaaattg aggaatcccc gatgccgatg tcgaatcgct caacacatct cgctgttggc
     2701 ctcatcgcgg gccttggcct tggttttctg ccacatccgg cggctgcgga agggcctgtg
     2761 ttcaaggtcg atccgtcctg gccaaagaca cttcccaaca attggattct ggggcagatc
     2821 ggcggactga ccgtggatgg acaggatcac atctggatcg atcaacggcc ccgctctctc
     2881 accaaggacg aagcaggtgc cgcccagaag ccgccgcagt ccaaatgctg catgcccgcg
     2941 ccgccgatca tggaattcga cgcggccggc aatttcctca aaggctgggg cggcccgggc
     3001 gaaggctatg attggcccga gcaggaacac gccgtccgcc tggacggcaa gggcaacatc
     3061 tatctcgcgg gcaatggtgc caaggatcag atgctgctga aattcaccac cgaagggaaa
     3121 ttcgtcaagc agctcggcaa actttccatg ccgctgggca gcaaggacac cgacagcgtc
     3181 gggcgcgtgg cggatatttt ctatgacgcc cctgccaatg agctttacct cgccgatggt
     3241 tatggaaacc atcgcgtgat ggtgatcgac gccgacagct tcgcgttcaa gcgcatgtgg
     3301 ggcgcctatg gcaagccacc ctcagacgac aacatcccga tgtacaatcc ggcttccgcc
     3361 caattcgcca atccggttca ttgcgtgaag atcgcgagcg acggcctggt ctatgtctgc
     3421 gaccgttcca acaaccggat tcaagtgttc cagaaaaacg gcaccttcgt gaaggagttc
     3481 gtctacgaca aggacacaag gcagtccggc tcgacctggg acatcgcgct ctggccggac
     3541 aagaacaata cctatctgtt gatggcggac ggcaccaaca atctgatccg cgtgatccgc
     3601 cgggccgacg gccaggtgct gagcacattc ggaaccggcg gaagacaggc cggccaattc
     3661 cactgggtgc atgtgatggc gatcgactcc aagggtaatc tctataccgg cgaagtcgat
     3721 accgggaagc gcatccagaa attcacgtcc aacatgacgc cctgattgcg agggtccatc
     3781 tcaacggacg ctacgatccg ttgaacatca aagggggagg tcacaccatg aagaaccgct
     3841 tgaaactgat acttgcgctc gttctcggcg cgctgatgtc gtcgtcatcg gcctttgccg
     3901 aagggcccga atataaagtc gacgcgtcat ggccgaagca gcttcccaat aattggatca
     3961 tcggccagat cggcgggatg aatgtggatg cgcaggatca tatctgggtg tttcagcggc
     4021 cgcgctcact gaaacccgtg gacctcgccg agacgaaaca cgcgaaatgc tgtatcgccg
     4081 cgccttcggt gatcgagttc gatgcggccg gcaatgtgct gtcgtcctgg ggcggccccg
     4141 gctacaaccc cgattggcca gaggtcgagc acggtatttt cgtcgacggc aagaacaata
     4201 tcctgctgac gggcagcggg aacaaggacg gcacgctgct gaaattcagc cgcgacggca
     4261 aattcctggc caaattcgga caccaggggc cgaccgccgg cagccaggat acaagcgcgc
     4321 ttggcggcac ggccagcatc gccttcgatc cgcaggcgaa cgaactcttt ctatccgacg
     4381 gctatttcaa tcaccgcgtc atcgtgctcg atgccgacag cctcgcctac aagcgcatgt
     4441 ggggcgctta tggcaagccg ccggcggata tgaagctgcc gaactacaat ccaaaatcgg
     4501 cacaattcgc aaatcctgtc cattgcgtgc gcatcgccaa tgacggcctc gtctatgtct
     4561 gcgaccggac caatgaccgc atccaggtgt tccagaagaa cggcaccttc gtgaaggaat
     4621 tcgcgatccg acctgaaacc cacggcaccg gctcatccta cgatcttgcc ttctggcccg
     4681 acaagaacca gacttatctc atcatcgccg atggcgcgga tggcgaagcg gtgatcgtgc
     4741 ggcgcgagga cggcaaggaa gtgggcgctt tcggccatta cggcaagcag gccggacaat
     4801 tccacaacat tcatcagatc gtgtcggatt cgaagggcaa tatctacacc ggcgaagtcg
     4861 atgtcgggat gcgggtgcag aaattcgtgc ccaacatgcc cccggcaaaa tgaatgcaag
     4921 cgaatagcga atggcgaatt gagctttatt cgctattcgc tattcgtctc tcgccgccca
     4981 ataacgacat cgtttcataa agcggcaggc cgacgacatt gctgtaggag ccattgatgg
     5041 atttcaccag cgcttcagcg cgaccctgga tggcatagcc accggccttg ccctcccctt
     5101 cccggctcgc cccatattcg gcgacctcgc gtggcgtaag aactttaaac gcgacccgcg
     5161 tcgtcaccac gcgcgtgcgc ggttttgcat tgggcgcgat cagcgcgagc gcggtcagca
     5221 cctgatgccg ccgcccggac aacacccgca gacagcgttc gacctccgct gcgtcctcgg
     5281 ccttgggaag gatccgccgc ccgcagacca ccaccgtgtc ggcggcaagg atgacaaact
     5341 cccctcctcg aaccgcttcg ccgtccgccc ctgcctcaag gggagggaga gataaaattt
     5401 tggccaccgc cgcggctttg gcgagcgcca gccgcaagca atagacccgc ggcagttcct
     5461 gcttcttcgg agtctcgtcc acgtcgggcg cgacgatacg ggcgagctgg attccagctt
     5521 gcgccagcag ggccgcacgc ctggggctcg cgctcgcgag aaccagcggc agcgatgagg
     5581 ctcgtgtcgc ggccacggct gtgggccgct ttctatttga agcggtaggt gatgcggccc
     5641 ttggtcagat cgtagggcgt catttcgac
//
LOCUS       DASYEG010000162         5363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_5126466, whole genome shotgun sequence.
ACCESSION   DASYEG010000162 DASYEG010000000
VERSION     DASYEG010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5126466"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..600)
                     /locus_tag="VGM72_07370"
     CDS             complement(97..600)
                     /locus_tag="VGM72_07370"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEY4265119.1"
                     /translation="MIFTIGHSNQTRDRFLDLVGQNAITALADVRSVPLSRWASQFNR
                     DALAKSLAERGIAYVFLGRELGGRPKDPAFLRDGRPDYAAMARSEGFRTGIARVREGA
                     KIYRLALMCAERDPIDCHRFLLIARHLAGHGVPVSHILASGTVEEQQATERRFHRRDE
                     NPDLFGN"
     gene            652..1011
                     /locus_tag="VGM72_07375"
     CDS             652..1011
                     /locus_tag="VGM72_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265120.1"
                     /translation="MRRFVPILACFVAAGSMPAQPARGASCADDYLTIETVRGRILEI
                     KPAPEPFKTADIYFSGPSPCDRIWMQVLKTDAARCRAGMPIVVTGVVTMDVENQSWEI
                     GPTKSEYMTPGVDFTCG"
     gene            1077..1310
                     /locus_tag="VGM72_07380"
     CDS             1077..1310
                     /locus_tag="VGM72_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265121.1"
                     /translation="MAGAIDVRLIPMVMAVFAVVNLSACAAIGVAETVAGTGAGIAVS
                     AISTTASVAGDVIGGAARTVTGSGSNPTPHDSQ"
     gene            complement(1322..2092)
                     /gene="gloB"
                     /locus_tag="VGM72_07385"
     CDS             complement(1322..2092)
                     /gene="gloB"
                     /locus_tag="VGM72_07385"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEY4265122.1"
                     /translation="MLPLEIEPVPCLRDNYAWLAYDREEGICAVVDPSEAEPVRAALK
                     SRGLTLTHILNTHHHPDHVGGNAALKAAFGAIIVGPGKDAARIPGIDVGVTEGETYAV
                     GRHVAKVLEIPAHTRGHIAFWFEADAAVFTGDTLFAMGCGRLFEGDPPTMWTSLSKLM
                     TLPDGTRVYCGHEYTEANGRFALSVEPGNTDLNARMTEVTAARALGRPTLPSTMGLER
                     KTNPFLRPSSPEIRRSLGLERAEDVDVFAETRRRKDVF"
     gene            2248..2994
                     /locus_tag="VGM72_07390"
     CDS             2248..2994
                     /locus_tag="VGM72_07390"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY4265123.1"
                     /translation="MALDVRTLEEFYAGRIGQVARRLILQRLRTLWPTLSGRRVLGLG
                     YAAPYLRPMVGDAERTIAAIPEALGVASWGVGGRSLTTVVDELALPFPDSFFDCQLVI
                     HGLEATEAQRPFLRELWRVLAPAGRMIVVAPNRASLWAQMETSPFGHGQPYSRGQLAR
                     LLDESLFRPEYWDAALFMPPFGGRRSLRGGRAWERIGARVWPRLAGVHIVEATKSLYA
                     PVGLVKARRRGVLKPAMVSNAPSKNMPSRR"
     gene            complement(2998..3636)
                     /gene="metW"
                     /locus_tag="VGM72_07395"
     CDS             complement(2998..3636)
                     /gene="metW"
                     /locus_tag="VGM72_07395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEY4265124.1"
                     /translation="MNQQTPIVPKPVGLRPDLAAIAAMIPPGTRVLDIGCGDGSLLEY
                     LVRAKNVDGRGLELIQHNVNQCVARGLSVVQGDADTDLSEYPSGIFDIVILSQTIQAT
                     EKPSTVLRELLRIGKRVIISFPNFGHWRVRLSLLISGRMPVTDSLGHSWHETPNIHLC
                     TISDFLALARDTGAQIETALALIDNERTRTMHPDAWGPNIFAQGAIFLLAKK"
     gene            complement(3633..4808)
                     /locus_tag="VGM72_07400"
     CDS             complement(3633..4808)
                     /locus_tag="VGM72_07400"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEY4265125.1"
                     /translation="MSDAAPPQFLRPLRAPAEDRGRVLELGPEQPLVLDSGKVLSPIQ
                     IAYMTYGTLNAARSNAVLVCHALSGDQFVASIHPVTGKPGWWSYAVGPGKPLDTDRFF
                     VICANVIGGCMGTTGPGEIDPATNEPYGLNFPLVTIRDMVRAQAMLLDSLGIDQLLCA
                     LGGSMGGMQVLQWAAAYPERVYSAMPLATAARHSAQNIAFHEVGRQAIMADPDWRGGD
                     YGLAGVRPAKGLAVARMAAHITYLSEAALQRKFGRELTDRNALSFGFDADFQIESYLR
                     HQGMTFVDRFDANSYLYITRAMDYFDLAADYGGVLAEAFRGTKTRFCLVSFTSDWLFP
                     TSENKRIAHALNAAGANVSFVEIESDRGHDAFLLDEPELFGTMRGFLGAAAAVKGVP"
     gene            complement(4862..>5363)
                     /locus_tag="VGM72_07405"
     CDS             complement(4862..>5363)
                     /locus_tag="VGM72_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265126.1"
                     /translation="ADILSDADLPRIRSFKPRSILCCNILEHVRDPKALARRCAEIVG
                     PGGLIFVTVPFSYPHHRDPIDTMFRPSPETLAELFRPAILIRGEIVDVGQSYRGQVKR
                     RPWLLLRHISRFPFPFIGFKGWKRSMTKLYWLFHNYRITGALFQVPPAQEPREALRVD
                     SGARAT"
BASE COUNT          983 a         1644 c         1768 g          968 t
ORIGIN      
        1 gtggtccttg gcatcgcgca cgcgaattac ggccacatgg cgatcgtgcc gcccgagacc
       61 cgcgccgaac tcgccaagga tttcgattag ccgccctcaa ttgccgaaaa ggtcagggtt
      121 ttcatcgcgc cgatgaaagc gccgctccgt tgcttgctgt tcttcaaccg tgccgctggc
      181 aaggatgtgg ctgactggaa caccgtgtcc ggccaggtgc cgggcgatca gcaggaagcg
      241 gtggcaatcg atcggatcgc gctcggcgca catcagcgca aggcgataga tcttcgcacc
      301 ttcgcgcacg cgcgcgatgc cggtacgaaa tccctcgctc cgcgccatcg cggcgtaatc
      361 gggcctgccg tctctcagaa atgccgggtc cttcggacgc ccgcccaact ccctgccgag
      421 gaacacatag gcgatgcccc tctcggcaag cgatttggca agcgcatccc tgttgaactg
      481 cgatgcccag cgcgacaacg gcacggagcg cacatccgcc aaagcggtga tcgcgttttg
      541 cccgaccaga tcgagaaaac ggtcccgcgt ctgattggaa tggccgatcg tgaagatcat
      601 tgcgtatccg ttgagggttt tgcgaaactg cgtctctggg aaagatgtcc gatgcgccgt
      661 ttcgttccca tccttgcgtg ttttgtcgct gccgggtcca tgcccgcgca gcctgcgcgc
      721 ggcgcgtcct gcgcggatga ttatctcacc atcgaaacgg tgcgcggcag gattctcgag
      781 atcaagcccg cgccggagcc gttcaaaacc gccgatatct atttttcggg gccgagcccg
      841 tgcgaccgga tatggatgca ggtgctcaag acggacgccg cgcgttgccg cgccggcatg
      901 cccattgtgg tcacgggcgt cgtcacaatg gatgtcgaaa accaatcctg ggaaatcggc
      961 cccaccaaaa gcgaatacat gacgccaggc gtggatttta cgtgcggctg agtgggccga
     1021 tcacattttt gcccgcctgc cttgtagatc tgcgcgtttc gctggagagt gcgtctatgg
     1081 cgggagcgat tgacgtgcgt cttattccca tggtcatggc cgtgtttgcg gtcgtaaatc
     1141 tctcggcttg cgccgcgatt ggcgtggcgg aaaccgtggc tgggacgggg gccgggatcg
     1201 cggtctcggc gatctccacc accgcaagtg ttgcgggcga tgtcatcggg ggagctgcgc
     1261 gcaccgtcac tggcagtgga agcaatccaa cgccgcacga ttcccaatag gaccctcgct
     1321 cttagaaaac gtccttgcgg cggcgcgtct cggcaaacac gtctacatct tcggcccgct
     1381 ccaaacccag ggagcggcgg atttccggtg aagatggccg caggaaggga ttggtcttgc
     1441 gctccaggcc catggtagag ggaagagtcg ggcggcctaa agcccgcgcg gccgtgacct
     1501 ccgtcatccg tgcgttcaga tcggtattgc cgggttcgac cgacagggca aagcggccat
     1561 tggcctcggt atattcatga ccgcaataga cccgtgtgcc gtctggaagc gtcatcagct
     1621 ttgaaaggct agtccacatc gtcggcggat cgccttcgaa cagacggccg caccccatcg
     1681 cgaacagcgt atcgccggtg aacaccgccg catcggcctc gaaccagaaa gcgatatgtc
     1741 cccgggtatg tgcggggatt tcgagcacct tcgccacatg gcgtcccacc gcataggttt
     1801 cgccctcggt gaccccgaca tcgattccgg gaatccgcgc ggcgtccttt cccggtccca
     1861 cgatgatcgc gccaaatgcc gccttcagcg ccgcattgcc gcccacatga tcgggatgat
     1921 gatgggtgtt gaggatatgg gtgagcgtga gcccacggct tttgagggct gcgcggaccg
     1981 gctcggcctc ggaagggtcg accaccgcgc atatcccttc ctcgcggtca taggcgagcc
     2041 aggcataatt gtccctgagg cagggcacag gttcgatttc aagcgggagc atgagacaat
     2101 gccttattgg gccgattctc ttccttcaaa aatgggcgaa gagaattcgg gcggatggga
     2161 gcctgaaatc atgctgggcc agccggagcg gatgcgcaac tggaagccgc gcattgagac
     2221 gaaactcttc gcggtctaag cattcccatg gcgctcgatg tccgcacgct ggaagaattc
     2281 tatgccggca ggatcggcca ggtggcacgc cgcctgattc ttcagcgtct gcgcaccctc
     2341 tggcccactc tgtcgggccg gcgggtgctc ggcctcggct atgccgcacc ctatctgcgc
     2401 cccatggtgg gggacgccga acgcaccatc gcagccattc ccgaagccct cggtgtggca
     2461 tcctggggcg taggcggacg cagcctcacc acggtcgtgg atgaattggc gctgccgttt
     2521 ccggattcct tcttcgattg ccagctcgtg atccacgggc tggaggccac cgaggcgcag
     2581 cgccccttcc tgcgggagct gtggcgtgtg ctcgcgccgg ccggccggat gatcgtcgtc
     2641 gcgcccaatc gcgcaagcct ctgggcgcag atggagacct ctccgttcgg gcacgggcag
     2701 ccctatagcc gaggccaatt ggcgaggctg ctcgatgaat cgctgttccg gcccgaatat
     2761 tgggacgcgg ctttgttcat gccgccgttc ggggggcgca gatcgttgcg ggggggaagg
     2821 gcctgggaac ggatcggggc ccgcgtgtgg ccccggctcg ctggggttca catcgttgag
     2881 gcgacgaaat cgctctacgc gccggtcggt ctggtgaagg cgcggcgccg gggcgtgctg
     2941 aagcccgcca tggtgagcaa cgcgccctcg aagaacatgc cttcgcggcg atgatcgcta
     3001 tttcttcgcc agcaggaaga tcgcgccttg cgcgaagatg ttcgggcccc acgcatcggg
     3061 atgcatggtg cgcgtgcgct cattgtcgat gagggcgagc gccgtttcga tctgggcgcc
     3121 ggtgtcgcgc gccagcgcga gaaaatccga gatggtgcag agatggatgt tcggcgtctc
     3181 gtgccagcta tggccgagcg aatcggtcac cggcatgcgc ccggaaatca gcagcgacag
     3241 acgcacccgc caatggccga aattgggaaa ggagatgatg acgcgcttgc cgatccgcag
     3301 cagttcccgc agcacggtgc ttggcttttc cgtcgcctgg atggtttgcg acaggatgac
     3361 gatgtcgaaa atgcccgacg gatattccga cagatcggtg tccgcatcgc cttgcacgac
     3421 cgaaagcccg cgcgccacgc attgattgac attgtgctgg ataagttcga gcccgcgccc
     3481 gtccacgttt ttggcgcgca ccagatattc caggagcgaa ccatcgccgc agccgatatc
     3541 gagcacgcgc gtgccggggg gaatcatcgc ggcgatggcc gcgagatcgg ggcgcaggcc
     3601 taccggtttt gggacgatcg gcgtctgctg gttcatggca cgcccttgac cgccgccgca
     3661 gcgccgagaa atccgcgcat ggtgccgaac aattcgggct catcgagcag gaacgcgtca
     3721 tgtccgcggt cgctctcgat ctcgacgaaa ctgacattgg cgccggccgc gttcagcgca
     3781 tgcgcgatgc gcttgttctc gctcgtggga aacagccaat cgctggtgaa cgagaccagg
     3841 cagaagcgcg tcttggtgcc acggaaggct tccgccagaa cgcccccata atcggccgcg
     3901 agatcgaaat aatccatcgc gcgcgtgatg tagagatagg aattcgcgtc gaaccggtcg
     3961 acgaaggtca tgccttgatg gcgcagatag gattcgatct ggaaatccgc atcgaacccg
     4021 aaggagagcg cattgcggtc ggtgagttcg cgtccgaatt tgcgctgcag ggcggcttcg
     4081 gaaagatagg tgatgtgggc ggccatgcgt gcgaccgcga ggcccttggc cggacgcaca
     4141 cccgccaggc cgtaatcgcc gccgcgccag tcgggatcgg ccatgatcgc ctggcgcccg
     4201 acttcgtgga aggcgatgtt ctgcgcggaa tggcgcgcgg ccgtggccag cggcatcgcc
     4261 gaatagaccc gttcgggata ggcggcggcc cattgcagga cctgcatgcc gcccatcgag
     4321 ccgccgagcg cgcacagaag ctgatcgatg ccgagcgaat ccagcagcat cgcctgcgcc
     4381 cgtaccatgt cgcggatggt gacaagcggg aagttcagcc catagggttc attggtggca
     4441 ggatcgatct cgcccgggcc cgtggtgccc atgcagccgc cgatcacatt ggcgcagatg
     4501 acgaagaagc ggtcggtgtc caggggcttg ccggggccga ccgcatagct ccaccacccc
     4561 ggcttgcccg tcaccggatg gatgctggcg acaaactgat cgccggacag cgcatgacag
     4621 acgagcaccg cgttggagcg cgccgcgttc agcgtcccat aagtcatata ggcgatctgg
     4681 atcggcgaca ggaccttgcc ggaatccagg acgagcggct gctccgggcc caattccagc
     4741 acccggccgc ggtcttcggc cggcgcccgc aaggggcgca ggaactgggg aggcgctgcg
     4801 tcggacaatc caagtctcct ttggccccca atgccccagg cgcgaaagag gggaccgtgt
     4861 gctaagtcgc gcgcgccccg ctgtcaacgc gcagggcttc gcgcggctcc tgcgcgggcg
     4921 gcacctggaa cagcgcgccc gtgatccggt aattgtggaa cagccaatag agcttggtca
     4981 tcgaacgttt ccagcctttg aagccgatga aaggaaaggg gaaacgggaa atgtgccgaa
     5041 gcaggagcca gggccggcgc ttgacctgtc cgcgatagga ttggcccaca tcgacgatct
     5101 cgccccggat caggatcgcg gggcggaaca gctcggccag ggtttccgga gagggtcgga
     5161 acatcgtgtc gatgggatcg cgatggtgcg ggtagctgaa tggcacggtg acgaagatga
     5221 gcccgccggg gccgacaatc tccgcgcagc gccgggcgag cgccttggga tcgcgcacat
     5281 gttccagaat gttacagcag agaatggacc tgggcttgaa actgcggatg cgcggcaggt
     5341 cggcgtcgga aaggatgtcg gcc
//
LOCUS       DASYEG010000163         5491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4691280, whole genome shotgun sequence.
ACCESSION   DASYEG010000163 DASYEG010000000
VERSION     DASYEG010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4691280"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..570)
                     /locus_tag="VGM72_07410"
     CDS             complement(40..570)
                     /locus_tag="VGM72_07410"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY4265127.1"
                     /translation="MARVSTIEEVSHPELADIMRRIRGARRGRLINVYKLLLHSPALA
                     ESWFAHNNAVRWKTGIDGRLRELVIIRIGYLTRVAYIVGQHVPHLAIPEGLSLAECEA
                     VKEWEAATLFGARDRAALAYADSMTREVRVPGQVFADLKPHFDERRIVELTVLIGTYS
                     MQARVMQALEIDPEQA"
     gene            597..1697
                     /locus_tag="VGM72_07415"
     CDS             597..1697
                     /locus_tag="VGM72_07415"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4265128.1"
                     /translation="MMRILVESDHFLKILPVILNPGTPEDHKRAVADFFAHDIDFPAW
                     CEKFRAEIPGLYPAKVEFAADQAELEAKLPDADAVIVESLVLRRDAVARLKPLAIVHK
                     FGALTANIDTQACAERRIMVFNVPRRGNVAVAEQAFALMIALAKGIGHYNGVVTADDL
                     AAKGHMIRPYDRRYTGGSNYARISDLRSLQGATLGIVGLGEVGREVAKRAGAFGMILL
                     YHQRNPIPPADELVLGARFAGLQDLMAQSDYILVQLPLNDSTRGIIGRTALQAIKPGA
                     MLINTARADLVDRDALMEALDTGRLAGVGMDVGYSEPWGAGDPLLKYKDGRVIAMPHT
                     AVGDRKIGLGDLAEMCRNIWRVLDNRRTGRRK"
     gene            1790..2452
                     /locus_tag="VGM72_07420"
     CDS             1790..2452
                     /locus_tag="VGM72_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265129.1"
                     /translation="MRVFASIAAVFVILSQPASAQSWMEYDYPEAGFSIHFPAAPKLA
                     SGLYETGAGAFVNAQIYSAYGPGNRFIVTVSNLSGTGVDQETALTQAVEAARSHGDVK
                     VDIPARVNGQRGRQLSIVGKDGGHSNLAIFFANNHLFQIEGTVLASNPDPQSGDAIRF
                     QQSLRFTGDYAGRRFAQAPGANGGGRGRFNGRGGGRFGRRGNVPMPAAMPPPPDQVPQ
                     TP"
     gene            3021..4265
                     /locus_tag="VGM72_07425"
     CDS             3021..4265
                     /locus_tag="VGM72_07425"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4265130.1"
                     /translation="MKTTYSTAVAENSLHVTTFTILFAISFSHLLNDMMQSLLAALYP
                     SLKARLELNFAQVGLITATYQLTASLLQPLVGLYSDRRPIPFSLPVAMVSTLIGLLVL
                     SVSGTYAMLLGASLLVGLGSAIFHPESARVARMASGGRFGLAQSLFQVGGNFGQALGP
                     LAAAAVVLNFGQASIAWFALMALASVIILYNVGIWYKHHGIARIRASAKLHQPPDLSR
                     TQITGAILVLLALIFSKQIYFSAIASYYTFYLIHHFGVSLRAAQLYLFLFLAPVAVGT
                     LLGGIIGDRIGRKYVIWFSVLGALPFSLLLPYMNLFWTGVMSVFIGFTLSSAFPAIVV
                     YAQELVPGKIGTISGLFFGFAFGLSGLGAAALGWIADRTSIEFVYAICSYLPILGLLA
                     ALLPDLNTAPKTSPLVAATAEE"
     gene            4351..5238
                     /locus_tag="VGM72_07430"
     CDS             4351..5238
                     /locus_tag="VGM72_07430"
                     /inference="COORDINATES: protein motif:HMM:NF018420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HEY4265131.1"
                     /translation="MFRIRMRSVLLAGAMAALAILPAPAAPAAPAGPVQVDLKLVIAT
                     DVSLSITDEEAQLEREGTAEVFLDPEVVRAIQSGALGSIAVAMLDWSTPNYDRVVLDW
                     TFVNDKASAAALAGKIRKLPRTPGTRTSISGALERAFLMLNDSDNQIVATRKVVDVSG
                     DGPNNDGISLQHIHDTTANNGIVVNGLPIMDPKAEGYFPDLDKYYAACVVAGKGAVVV
                     VVKKYEDFGAAMRHKLVLEVSQNESQIQRTLGEMQANPLLRKTATGNAPPVQPQLLRP
                     EKTYPGGCDKYGGFGYGGY"
     gene            complement(5251..>5491)
                     /locus_tag="VGM72_07435"
     CDS             complement(5251..>5491)
                     /locus_tag="VGM72_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265132.1"
                     /translation="KGERNRVTVKIALVKESQDWVLSVADDGPGFTFRETDRRASGLG
                     FVSGLARQLRGALEITADRGARCVVRFGGRHNADS"
BASE COUNT         1012 a         1697 c         1653 g         1129 t
ORIGIN      
        1 ggggccggtg ccgagctttc gtaaccaagt ttaacgcgct caggcctgtt cgggatcgat
       61 ttcgagcgcc tgcatcacgc gggcctgcat gctgtaggtg ccgatcagca cggtgagttc
      121 cacaatgcgc cgttcgtcga aatggggctt caggtccgca aacacctggc ccggcacccg
      181 gacctcgcgc gtcatcgaat ccgcataggc gagcgcggcc cggtcacgcg ctccgaacag
      241 cgtcgctgcc tcccactcct tcacggcctc gcattccgcg agcgagagcc cctcggggat
      301 ggcgagatgg gggacatgct gacccacgat ataggcaacg cgtgtcagat agccgatccg
      361 gatgatcacc agctcccgca gacggccgtc aattcctgtc ttccagcgca cggcgttatt
      421 gtgcgcgaac cagctctccg ctagagctgg actgtgcagg agcaatttgt aaacgttgat
      481 caggcggccg cgccgcgcgc cgcgaattcg ccgcatgata tccgcgagct cgggatgact
      541 gacttcttcg attgtggaaa cacgcgccat tttcgccatc gttcgaggta cgctccatga
      601 tgcgcatcct agtggagagc gatcattttc tcaaaatcct gccggtgatt ctcaaccctg
      661 ggacgccgga agaccataag cgagccgttg ccgatttctt cgcgcacgac atcgactttc
      721 cggcctggtg cgagaaattt cgcgcggaaa taccgggcct ctatccagcc aaagtggaat
      781 tcgccgccga tcaggcggag ctcgaagcaa aattgccgga tgcggacgcg gtgatcgtcg
      841 agagcctggt gttgcggcga gacgcggtgg caaggctgaa accgctcgcc atcgtgcaca
      901 aattcggcgc cctcaccgcg aacatcgata cgcaggcatg cgcagaacgc cgcatcatgg
      961 tgttcaacgt gccccggcgg ggcaatgtcg cggtggccga gcaggctttc gcgctgatga
     1021 tcgcgcttgc caaggggatc ggccattaca acggcgtggt gacggcggac gatctcgctg
     1081 cgaaggggca tatgattcgg ccctatgacc ggcgctacac aggcggttcg aattacgccc
     1141 gcatttcgga cttgcgctca cttcagggcg caacgctcgg cattgtgggg ctcggcgagg
     1201 tcgggcgcga agtcgcaaag cgtgcgggcg cgttcgggat gatcctcctt tatcatcagc
     1261 gcaatccgat tccaccggcc gacgaactcg ttctgggggc gcgctttgcc gggcttcaag
     1321 acctcatggc gcaatcggac tacatcctcg tgcagttgcc gctgaacgat tcgacgcgcg
     1381 ggatcatcgg caggacagcg ctgcaggcga tcaagccggg tgcgatgctg atcaacacgg
     1441 cacgcgcgga tttggtggat cgcgatgcgc tgatggaggc gctggatacc ggacggctcg
     1501 ctggcgtcgg aatggatgtc ggatacagcg agccctgggg ggccggcgac cctttgctga
     1561 aatataagga cgggcgcgtc attgccatgc cgcacaccgc ggttggcgac cgcaagatcg
     1621 gcctcggaga tcttgcggaa atgtgccgca acatctggcg cgtgctcgac aatcgccgga
     1681 ccgggcgcag gaagtaaccg tcagctaagc caacaaagat gtaaggaagc ggttgggctg
     1741 ggctcatctg gccatgccaa gctttgggcg tccccagatg agagacccca tgcgtgtgtt
     1801 tgcgtcgatt gctgctgttt tcgtcatcct gtcgcaacct gcatccgcac aaagctggat
     1861 ggaatatgat tatcccgagg cgggattctc aatccacttc cccgcagcgc cgaaactggc
     1921 aagcgggttg tatgagaccg gcgccggcgc ctttgtgaat gcgcagatct attcggctta
     1981 cgggccgggc aatcgtttca tcgtgacggt atccaatctt tcgggcaccg gcgtggacca
     2041 ggaaacggcc ctgacgcaag cggtggaagc cgcgcgctcc catggcgatg tcaaggtgga
     2101 tatccctgcg cgcgtgaacg ggcagcgcgg ccgtcagctc agcatcgtgg gcaaggacgg
     2161 cggccattcg aatctggcga tcttcttcgc caacaatcat ctgtttcaaa tcgaaggcac
     2221 ggtgcttgcc tccaatcccg acccgcaatc gggcgatgcg atccggttcc agcaatcgtt
     2281 gcgtttcacc ggcgactatg cgggacggag attcgcacag gcgccggggg ccaatggagg
     2341 cgggcgcgga cgattcaatg gccgcggcgg tggacgtttc gggagacgcg gaaatgttcc
     2401 catgcccgcc gccatgccgc cccctccaga ccaagtcccg cagacgccct agccccgcct
     2461 gccggcccac gacgataatc atctgtatac ggattatttc ggcaggccgc ggtcaaaaac
     2521 gcgaggggtg gcgtgaggcg ccgtatccgg cgtcaaaacg cgcgttttta agaaaagctg
     2581 ccgtcttgcg gccctcacgc ccggcggttt ttggctcgca ctttttccgg tttcgccgga
     2641 gagcacatgg cccgcccggc ttatcggcaa cccgtcctcc cgcccgccca ttctgtcgaa
     2701 agggtagcgg gtcggccgga aaatgcggcc ctcgcatcct tccttgacga ggccctcatg
     2761 gagcaccttg ccgccgaagt ttttcccatc cgtccgttac gcggaatggg agatgcaacg
     2821 ccagccggga ccttacgctg aagacggtcg acaacgcccc ctcatcaggt tccggttttg
     2881 accatgtagg aatcgatgtg tctggttgga agaggagcga gtcgctcatt ctttcattta
     2941 ccaagaagaa actgttgttt gctgccggga cggtctattt tacggggcac gcaaactcac
     3001 gattcgaaag ccctcttcga ttgaaaacca cctattcgac cgccgtcgcg gagaactccc
     3061 tccacgtcac cacgtttacg atcctgttcg cgatcagctt cagtcatttg ttgaacgaca
     3121 tgatgcagtc gctgctggcg gcgctctatc ccagcctgaa ggcaaggctg gaactcaatt
     3181 tcgcgcaggt ggggctgatc accgccacct accagcttac cgcgtccttg ctacagcctt
     3241 tggtcgggct ttacagcgat aggcgtccga ttccgttttc gctgcccgtc gccatggtat
     3301 ccacgcttat cggattgctg gtgctgtcgg tcagcggcac ctatgcgatg cttctggggg
     3361 cctcgctgct cgtaggactt ggctcggcga ttttccatcc cgaatccgca cgcgtggcgc
     3421 gcatggcctc cggcggccgt ttcggcttgg cgcaatcgct gttccaggtc ggcggaaatt
     3481 tcggacaggc gctcgggccg ttggcggccg ccgccgtggt gctgaatttt gggcaggcgt
     3541 ccatcgcctg gttcgcgctg atggcgctcg cttcggtcat catcctttac aatgtcggga
     3601 tttggtacaa gcaccacggc atcgcgcgca tcagggcgag cgcgaagctg caccaaccgc
     3661 cggatctctc acggactcag atcacgggcg cgatcctggt attgctggct cttatctttt
     3721 ccaagcagat atatttttcc gcgatcgcgt cctattacac gttttatctg atccatcatt
     3781 tcggcgtgtc gctgcgggcc gcccagctct atctgttcct gttcctggcg ccggtcgccg
     3841 tcggcacgct gctgggcggg atcatcggcg atcgcatcgg acgcaaatat gtgatctggt
     3901 tttcggttct gggcgcgctg ccgttctctc tgcttctgcc ttatatgaat ttgttctgga
     3961 ccggcgtcat gtccgtgttc atcggcttca ccctgtcgtc cgccttcccg gcgatcgtcg
     4021 tgtacgccca ggaattggtc cccggaaaaa tcggaaccat ttcgggcctg tttttcggtt
     4081 ttgcgttcgg actgtcgggt ctgggcgcgg ccgcgctcgg atggatcgcc gatcggacaa
     4141 gtatcgaatt cgtctatgca atctgttcct atcttcccat cctcggtctg ctcgccgcct
     4201 tattgccgga cctcaatacg gctcccaaaa catccccgct cgtcgctgcc acggccgaag
     4261 agtgagcgtt gccgcgcgcg atgttgcggg ctaatgtttc gtggctcggg ttcgtttttc
     4321 ccaaggcatg cctaagatgg aaacgtcccg atgttccgta tccggatgag atccgttctg
     4381 cttgcgggcg ccatggcggc acttgccatt ctgccggctc cggcggctcc ggcagctccg
     4441 gcgggaccgg ttcaggtcga cctgaagctc gtcatcgcga cggacgtctc gctcagcatc
     4501 accgatgagg aagctcagct cgaacgggaa ggaaccgcgg aagtcttcct cgatcccgaa
     4561 gtggtccggg caatccagtc gggcgccctt ggaagcatcg cggtcgccat gctcgattgg
     4621 tccacgccca attacgatcg cgtcgtgctg gattggacat tcgtgaacga caaagccagc
     4681 gctgccgcgc tggccgggaa aatccgcaag ctcccgcgca cgccaggcac gcgcacatcg
     4741 atcagcggtg cgctggaacg cgcgttcctc atgctgaacg acagcgacaa ccagatcgtc
     4801 gcgacacgca aagtggtcga tgtttccggc gatggcccga acaatgacgg catctcgctg
     4861 caacacatcc acgatacgac cgccaacaac ggcatcgtgg tgaacggcct gccgatcatg
     4921 gacccaaagg ccgagggcta ttttcccgac ctcgacaaat attacgccgc atgcgtggtc
     4981 gcgggaaaag gcgccgtcgt cgtggtggtc aagaaatacg aggatttcgg agccgccatg
     5041 cggcacaagc tggtgctgga ggtttcgcag aacgaaagcc agatccaacg cacattgggg
     5101 gagatgcagg ccaatccgtt attgcggaag acggctacgg gaaatgcacc gcccgtccaa
     5161 ccccagctcc tgcgtccgga gaaaacctac cccggcggct gcgacaaata tggcggtttc
     5221 ggctatggag gatattgacc ggaacgcgcg tcaggaatct gcgttgtgcc ggccgccaaa
     5281 acgcacgacg cagcgcgcgc cccgatcggc ggtgatttcc agcgcccccc gcagttgacg
     5341 agcaagcccg ctgacaaagc ccaaacccga cgcccgccga tctgtctcgc ggaacgtaaa
     5401 gcccggtcca tcgtcggcca cgctcagcac ccaatcctgg ctttccttta cgagcgctat
     5461 tttaaccgtc acgcggttgc gttcgccttt g
//
LOCUS       DASYEG010000164        18902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1501397, whole genome shotgun sequence.
ACCESSION   DASYEG010000164 DASYEG010000000
VERSION     DASYEG010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 18902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18902
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501397"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2215
                     /locus_tag="VGM72_07440"
     CDS             <1..2215
                     /locus_tag="VGM72_07440"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY4265133.1"
                     /translation="LVNWDPRLQTAVSDLEVESIEVKGKLWHIKYPLAGGATFQWPVF
                     DEDGKQTGTETRDYIVVATTRPETMLGDVAVAVHPDDARYKALVGQHAVLPLADRRIP
                     IIADEYSDPKKGSGAVKITPAHDFNDFEVGKRHGLAPLNILTKEAALNDAVPLAYRGS
                     DRFEARKRVIADLEALGLIEKTEDIVHAVPHDEKTKTVVLEPLLTEQWYLNVTPLAEK
                     AIHAVEAGRTRFVPEHWANVYFNWMRNIQPWCISRQLWWGHQIPVWYGPDAHVFVEET
                     EDAARAAAAKHYGRETALTRDPDVLDTWFSSALWPFSTMGWPDETPELKLYYPTSVLV
                     TGFDIIFFWVARMMMMGLHFMGDVPFHDVFIHTRVLDEKGQKMSKTKGNVVDPLDIID
                     EYGADALRFALAIAAGQGRDIRLGLNRVETCRNFGTKLWNAARFCEMNECVRKREFDP
                     ASVDTTVNKWIVAETARAAAEVTAALEAYRFNEAAGAIYHFVWDVFCDWYVEFIKPVL
                     NGNDEDAKAETRDTAAWALDRILQLLHPFMPFITEELWARIAEHATPRRSQLMLSSWP
                     DLSGLPKHEDARAEMNWLIDLISGVRSVRAEMNVPPSARIVLLLKDAGAQTTARLGRH
                     RDVALTLARLSTARTAFDIPQGSAQFVLGEAVAALPLGDVIDLDKERARLAKDLKKAE
                     DEIAKFDAKLNNAAFVAKAPEEVIDEQKERRAEAAGVAGRLREALKRLSGGGDNA"
     gene            2208..4385
                     /gene="glyS"
                     /locus_tag="VGM72_07445"
     CDS             2208..4385
                     /gene="glyS"
                     /locus_tag="VGM72_07445"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEY4265134.1"
                     /translation="MPDLLLELFSEEIPARMQVQAAKDLERLVVGALSDRGLLFEAAR
                     GFAGPRRLTLAIQGLPVKQPDVSEEKKGPRVGAPEKAIEGFLKSAGVTLDQCETRSDV
                     KGEFYLAVIARKGRATTDVLSEILPEQIAKLPWPKSMRWGDGNFRWVRPLHGIVATFE
                     GEVVPFEVADVKSGNVTRGHRFLSSGEITVRHFEDYEKKLRDAHVILDGAERREIILH
                     AAQQKAFALGLELIPDEGLANEVMGLAEWPVVLIGQIDPEFMDVPPEILQTSMRTHQK
                     YFSLRDPARGKLANRFVAVANMAADDGGYEIVAGNERVLRARLSDAKFFWDQDRKRTL
                     ESRVDDLKNIVFHAKLGTQLERVDRIEALAGEIAKIIGAALGEAERNKLIEKAKRAAR
                     LCKADLTTGVVGEFPELQGVMGRYYALHYDEAPEVADAIRDHYKPLGPSDAVPTAPVS
                     IAVALADKLDTLVGFFAIDEKPTGSGDPYALRRAGLGVIRVLSNCTLRIELVQLLASH
                     EDRIIKSILTVTAQAALKLPGNTLFPPHIRVSPRNTALQNSTQISLDILAFFADRLKV
                     ALREKGVRHDLIDAVFALGSEEDLVRLVRRVEALQAFLKTDDGANLLTGYRRAANILK
                     IEEKKDKRDYRGVPDGLKQDEEIALQRALTHAKVEMAKALAAEDFADAMRALSSLRVP
                     VDHFFDKVTVNTDDKTLRENRLMLLSQIVETAHQVADFSKVEG"
     gene            4807..7482
                     /gene="ppdK"
                     /locus_tag="VGM72_07450"
     CDS             4807..7482
                     /gene="ppdK"
                     /locus_tag="VGM72_07450"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEY4265135.1"
                     /translation="MTQWVYNFGAGAADGRADLRALLGGKGANLAEMSNLGLPVPPGF
                     TITTEVCTYFYKNGQNYPDDLKQAVEAALTQVGKLADGRFGDAVRPLLVSVRSGARAS
                     MPGMMDTVLNLGLNPGTVEGLARQTGDERFAYDSYRRFIQMYSGVVLEVDHSHFEEIL
                     ENEKEAKGIELDTDLDAADWKRIAALYKAKVKQVLGKPFPDDVNEQLWCAIGAVFGSW
                     MGARAKTYRRLNNIPEDWGTAVNVQAMVFGNRGPTSATGVAFTRDPSTGERALYGEFL
                     INAQGEDVVAGIRTPQSITKRAREASRSKRASMEEAMPNAFVELSRIADALEAHYRDM
                     QDVEFTVQEDKLFMLQTRSGKRTAKAALRIATEMVREQRITQDEAVMRVDPSALDQLL
                     HPTLDPNAPKELIASGLAASPGAASGEAVFTADEAERAASDGRDVILIRLETSPEDIH
                     GMHAAKGILTVRGGMTSHAAVVARGMGRPCVTGAGTLKIDVERGAMSTGTMTISRGDI
                     VTIDGAAGKVYRGRVPTLHPEVSGDFATLMGWADARRRLKIRTNADTPSDCETALRYG
                     AEGVGLCRTEHMFFDAERILAVRQMILADDLAGRKAALAKLLPMQRGDFHKIFRLMAG
                     LPVTIRLLDPPLHEFIPHSDEEIAEVAKAAGSEPAKLRARAMALAESNPMLGHRGCRL
                     GITYPEIYEMQARAILEAALDAENENGKPVELEIMIPLVSFKSELDYLKERIEAVAQA
                     IKTECGKAPSYMIGTMIELPRAALMAKQIAETAEFFSFGTNDLTQTALGVSRDDAGSF
                     LSEYLARGFIERDPFVSIDRDGVGELVEIAVKRGRSARPKLKLGICGEHGGDPDSIRF
                     CHEVGLDYVSCSPFRVPVARLAAAQAALATAREKD"
     gene            7645..8352
                     /locus_tag="VGM72_07455"
     CDS             7645..8352
                     /locus_tag="VGM72_07455"
                     /inference="COORDINATES: protein motif:HMM:NF019128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEY4265136.1"
                     /translation="MMNGVGNAHRRADCVVVGAIALLAVSSVSAHEFNLYTYELEARA
                     ALPDYVAVTQSPVALARAYAAAESDCLAQVLYYEARGEGLDGEKAVAEVVLQRTVNRD
                     YPETVCGVAYDGVQPDRRDCQFSFACDGSLRKPKDAVAWTRIRQLADKIMTGAIRLSG
                     ATGHAIAYHSVDVAPAWADSMLKTATIGNHVFYKRDPMAQMRLAQAVAKPIESLSGMF
                     TPAMTTGPAVPSEEVQP"
     gene            complement(8332..9222)
                     /gene="nadC"
                     /locus_tag="VGM72_07460"
     CDS             complement(8332..9222)
                     /gene="nadC"
                     /locus_tag="VGM72_07460"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEY4265137.1"
                     /translation="MAAGLSQQENRLALPHTNPPHALLIEPLVRRALEEDLGRAGDIT
                     TDAIIPQGQIARAEIAARETGVICGLIAADITFRLIDPAIVFTVNLPDGSSAAEGDIV
                     AALEGPARAILTAERVALNFIGHLSGVATATHRLVSAVAGTNARIICTRKTTPGLRIL
                     EKYAVRCGGGFNHRFGLDDAVLVKDNHIAAAGGIEAVVRALRGRLGPMVRIEVEADTL
                     EQLESALALGLDAILLDNMPPETMKRAVAITRGRARLEASGGVTLERVRAIAESGVDY
                     ISSGAITHSARNLDLGLDFL"
     gene            complement(9222..10262)
                     /gene="nadA"
                     /locus_tag="VGM72_07465"
     CDS             complement(9222..10262)
                     /gene="nadA"
                     /locus_tag="VGM72_07465"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006879.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEY4265138.1"
                     /translation="MKNLPYTPEIAAATAPFYARVKQVVPEIEWPVFAPYVAAIHALK
                     RERHAVILAHNYMTPEIFHCVADFVGDSLQLAREAAKTDAKIIVQAGVHFMAETAKIL
                     SPHKKVLIPDVEAGCSLAASINGADVRLLRQNYPGLPIITYVNTSAEVKAESDICCTS
                     SNAVDVVESIAAEWGVDSVIMIPDEFLAQNVAKQTTVKIITWKGHCEVHERFTAEDVR
                     NLRSANPGLIVLAHPECPPEVVAEADFAGSTSGMIDYVGQKRPKRVVLMTECSMSDNV
                     AVEYPDVSFVRPCNLCPHMKRITLPKILHSLETMTTEVSVEPLIAARARAAVEAMLKV
                     KPRARGLQREAA"
     gene            complement(10383..11390)
                     /locus_tag="VGM72_07470"
     CDS             complement(10383..11390)
                     /locus_tag="VGM72_07470"
                     /inference="COORDINATES: protein motif:HMM:NF040269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265139.1"
                     /translation="MASARKFTDDAKNNEVTPGEAQTVLIGLSAAIVSVAEERPRVLV
                     MHRGEDADALPFGTFDPLSHRTLESGLRNWVEEQTRLQLGYVEQLYTFGDRGRHMTRP
                     GEGPRVLSVGYLALTRPQQGRGPETLWHDWYGYFPWEDWREKKPPIIDALIMPRLAQF
                     VDSASAKDLRVSRQDRIRLCFGGDGIAWDGEKVLERYELLYEAGLVKEAQRDRQAAPA
                     GGPVLPLGSAMVFDHRRILATAMGRLRGKMKYRPVVFELMPPEFTLLELQRTVEALSG
                     IRLHKQNFRRLVEAQGLVEGTGRLSRKAGGRPAEQFRFRREVLRERPTPGARLTSSRL
                     R"
     gene            complement(11390..11911)
                     /locus_tag="VGM72_07475"
     CDS             complement(11390..11911)
                     /locus_tag="VGM72_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine phosphatase family protein"
                     /protein_id="HEY4265140.1"
                     /translation="MSHILIAPYADLEETVRRHRPSHLVTLMIEPFVETPKAIHPDHH
                     LRIGVHDVIEPSEGSVVPEPHHIAEMLAFAKNWDRRAPFLVHCWAGISRSTAAAYIVL
                     CNLHGQGHELHIARALRFRAPHAQPNPLMIRHADRLLGREGRMIAGVEAMGEGRPNWQ
                     SEIVELPLDLDLL"
     gene            complement(11985..12575)
                     /locus_tag="VGM72_07480"
     CDS             complement(11985..12575)
                     /locus_tag="VGM72_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008035435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HEY4265141.1"
                     /translation="MKDRANPARAWQRMLSGRRLDLLDPSPLDIEIDDIAHGLARVAR
                     WNGQTKGAHAFSVAQHCVVVERFVHELKPKASRALRLAALLHDAAEYVIGDLISPFKM
                     AVGLDYKAFENRLARAIHIRFGLPPENTPEESGVIKRADRMSAYFEAIQLAGFTVTES
                     RKFFGTPSGMVPPRLVPVPAVDAQTLYLERFHRLVA"
     gene            12758..13510
                     /locus_tag="VGM72_07485"
     CDS             12758..13510
                     /locus_tag="VGM72_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY4265142.1"
                     /translation="MRPDLAEALAFFTDGISGVVVEEVVKATKDAGDEAARRVEAAID
                     AICTANGITRVTQPARSNAVTMSLREVQGNLGDLLTAAARLSDLVVFGPIRENDQAGL
                     AEAFVQVLVETDRPVLRATDEAPKSFARRIAIGWDGKTAAAQAVSAAIPYFTKADSVE
                     ILSIQRPPLKAEATEGLKEYLSLHGVQSTERLIDPGSKRIGEVLLDAATQGDADLLVV
                     GGYGRGRLRESLIGGVTRHVIAHAGLSVFMVH"
     gene            13666..15045
                     /locus_tag="VGM72_07490"
     CDS             13666..15045
                     /locus_tag="VGM72_07490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEY4265143.1"
                     /translation="MTVGTLSSPSSTLLHGLAHIVSRWSFTLSLALGFATGLTLLPVA
                     GGELTGAIISFGAICIAAAAARQALDRKTQIEGLEQQLLDETRYHAFVDAAAEGFFRT
                     TREGRYVIANPALARIYGYESPEHLCAELTDIGQTLYVDVNRRAEFQRLISANARVKD
                     FVSQIRQRDGTLIWISENARTVRDEDDQFLFYEGTVEDITSQRESEEATRRALVETQE
                     AVRAKAAFLAAMSHELKTPLNAVIGFSDLMRQELFGPVNEPRYRGYIADIHDNGRRLL
                     GMINDILDLSRIESHLLDLDDDLVCVQEAVRVAYATIVEGKSDAAPIAFDLPAHVPFL
                     KGDPRRLHQILAHLLSNAVKFTPPKGQITVRVYLTKGAEIAIMIRDTGIGMEPGRIAH
                     ALEPFKQLDSSLSRRFEGVGLGLPLANALVRLHGGTLTIESVPGEGTIVTICFPPERS
                     VETVRAAIA"
     gene            15086..15796
                     /gene="queC"
                     /locus_tag="VGM72_07495"
     CDS             15086..15796
                     /gene="queC"
                     /locus_tag="VGM72_07495"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEY4265144.1"
                     /translation="MSVKNRKALVLFSGGQDSTTCLAWALSQFDRVETVGFAYGQRHA
                     AELDARPNILRRIRDEFFQWRDRLGEDHVLVLDVLSQIGGSALTDEIAIAMRSDGLPT
                     TFVPGRNLMFLTAAAALGTRREAYDLVGGVCETDYSGYPDCRDETMQAMQRAITLGFA
                     QAFTIHTPLMKIDKAETWRVAERLGGEVLVNIIIEDTVTCYQGDRSHRQVWGYGCGAC
                     PACDLRAAGYLRYRGVAA"
     gene            15793..16425
                     /gene="queE"
                     /locus_tag="VGM72_07500"
     CDS             15793..16425
                     /gene="queE"
                     /locus_tag="VGM72_07500"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR04508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HEY4265145.1"
                     /translation="MTYQVKEIYYTLQGEGARAGRAAVFLRFAGCNLWSGREEDRAAA
                     ICKFCDTDFVGTDGPGGGKFADAESLANAVLSKWPADQGKPYVVCTGGEPLLQLDSAL
                     IRALHDAGFEVGIETNGTLKPPTGIDWICVSPKAGADLALLTGNELKLIYPQTGAEPE
                     RYTGLAFEHYFLQPMDSPERAQNTAAATQYCLAHPKWRLSLQTHKLIGIR"
     gene            16473..16847
                     /locus_tag="VGM72_07505"
     CDS             16473..16847
                     /locus_tag="VGM72_07505"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEY4265146.1"
                     /translation="MIEITQQFGFDAAHYLEDAPSASNRRMHGHSFYAEVTLRGMPDP
                     VKGWVRDFTEINTALSDIRARLDHQLLNDIAGLGAPTLENLTRYIFREARALMPEVCR
                     VRLSRPSSGQSCVFEEPGSAAQ"
     gene            16834..17292
                     /gene="queF"
                     /locus_tag="VGM72_07510"
     CDS             16834..17292
                     /gene="queF"
                     /locus_tag="VGM72_07510"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEY4265147.1"
                     /translation="MPRNKSPLQLGRLVAVPASPEQAVLDAVSNPHADTDYVARFTAP
                     EFTTLCPITGQPDFAHFVIDYCPAKSLLESKSLKLFLAAFRNVAGFHEDCTVTIGKRI
                     VTAIRPKFLRIAGFWYPRGGIPIDVFWQHGRLPKNVWLPDLEVEPYRGRG"
     gene            complement(17274..17873)
                     /locus_tag="VGM72_07515"
     CDS             complement(17274..17873)
                     /locus_tag="VGM72_07515"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEY4265148.1"
                     /translation="MSEQSRCPWPGEDPLYRAYHDEEWGVPEFDDRALYEKLVLDGFQ
                     AGLAWITILRKREDFRRAFQGFKPEKIARYGAKDMKRLLEDASIVRSRLKIEAAITGA
                     RLWLDIMEKEPGGFSDLIWKHVDGKPRTNRLKRIEDIPAKTEMSERLAKELKGRGFKF
                     CGPVIVYAFAQATGMVNDHLVSCHRHAACAKLAAQPRPR"
     gene            complement(17866..18708)
                     /locus_tag="VGM72_07520"
     CDS             complement(17866..18708)
                     /locus_tag="VGM72_07520"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265149.1"
                     /translation="MIHASFLDDRAVLAHGGPEARDFLQGLTTNDVTTVAEEQPVYTA
                     LLTPQGKILFDFFVVPDAEGRLLIDCAASRATDLMKRLTLYKLRAKVKIAHPTDLALA
                     AAWDDNGAFEIPGDVVRFRDPRHPSLGVRMIGARNALANAIGGLAASDYQLHRLVLGV
                     PDSADLTPDSVFALDAGLEELNGVSFKKGCFIGQEVTARMKHRAAARRRFFVAEFAGE
                     APAPGTPIISEERELATFATGANGRALALVRLDRWAEAEAKRAPVTADGREVQLNKPE
                     WLHV"
BASE COUNT         3812 a         5559 c         5747 g         3784 t
ORIGIN      
        1 cctcgtcaat tgggacccgc ggctgcagac cgccgtctcc gatctcgaag tcgagagcat
       61 cgaggtcaag ggcaagctct ggcatatcaa atatccgctc gccggcggcg cgaccttcca
      121 atggccggtc ttcgatgaag acggcaaaca gaccggcacg gaaacgcgcg actatattgt
      181 cgtcgcgacc acgcgacccg agacgatgct gggcgatgtc gcggtcgcgg tgcatccgga
      241 cgatgcgcgc tacaaggcgc tggtgggcca acacgcggtg ctgccgctgg cggaccggcg
      301 cattccgatc atcgcggatg aatattccga ccccaagaaa ggctcgggcg cggtgaagat
      361 cacgcccgcg cacgatttca acgatttcga agtcggcaag cggcatgggc tagcgccgct
      421 gaatattctc acgaaggaag ctgcgctcaa cgatgccgtg ccgcttgcct atcgcggatc
      481 cgaccgcttc gaggcgagga agcgcgtgat cgccgatctg gaggcgctcg gcctcatcga
      541 aaagaccgaa gacatcgtcc acgccgttcc ccatgacgag aagaccaaga ctgtcgtgct
      601 cgagcctcta ctcacggagc aatggtatct aaacgtgacg ccgttggccg agaaggcgat
      661 tcatgcggtg gaagcggggc gcacgcgttt tgtgcccgag cattgggcaa acgtctattt
      721 caactggatg cgcaacatcc agccctggtg catttcgcgc cagctttggt ggggtcacca
      781 gatacccgtg tggtatgggc ccgacgctca tgtctttgtc gaagagaccg aagatgccgc
      841 gcgcgccgcg gcagcaaagc attatggccg tgagaccgcg ttgacgcgcg atcccgatgt
      901 gctcgacact tggttctcct ccgcgctgtg gccgttctcg accatgggct ggcccgacga
      961 gacgccggag ctgaagctct attatccgac ctcggtgctc gtcaccggct tcgacatcat
     1021 ctttttctgg gtcgcccgca tgatgatgat gggcctgcat ttcatgggcg atgtgccgtt
     1081 ccacgatgtc ttcatccaca cccgcgtcct cgacgagaag gggcagaaga tgtcgaagac
     1141 caaaggcaat gtcgtcgatc cgctcgacat catcgatgaa tatggcgccg atgcgttgcg
     1201 cttcgcgctc gccatcgcgg cggggcaggg acgcgacatc cgcctcggcc tgaaccgggt
     1261 ggagacctgc cgcaatttcg ggaccaagct gtggaacgcg gcgcgttttt gcgagatgaa
     1321 cgaatgcgtg cgcaagcgcg aattcgatcc cgcctcggtg gatacgaccg tcaacaaatg
     1381 gatcgtggcg gaaaccgcgc gggccgccgc cgaagtgacc gccgctctcg aagcgtatcg
     1441 cttcaacgag gccgcgggcg cgatctatca tttcgtctgg gacgtgttct gcgattggta
     1501 tgtcgaattc atcaagccgg tgctgaacgg caatgacgaa gacgcaaaag ccgagacccg
     1561 tgacaccgcc gcctgggcgc tcgaccgcat cctgcaattg ctgcatccgt tcatgccgtt
     1621 catcaccgag gaattgtggg cgcgcatcgc cgaacatgcg acgccacggc gctcacaact
     1681 gatgttgtcg tcctggcccg atctgtccgg tctgcccaag catgaggacg cgcgggcgga
     1741 gatgaactgg ctcatcgatt tgatttccgg tgtgcgtagt gtgcgcgcgg agatgaatgt
     1801 gccgccttcc gcgcgcatcg tgctgctgct taaggatgcg ggggcgcaga ccacggccag
     1861 gctgggacgc catcgcgatg tcgcactcac cctggcccgg ctctcaacgg cgcgcacggc
     1921 gttcgacatt ccgcaaggtt ccgcgcaatt cgttctgggc gaggcggtgg ccgcacttcc
     1981 cttgggcgac gtgatcgacc tcgacaagga acgcgcgcgc ctggcgaaag atttgaaaaa
     2041 ggccgaggac gagatcgcga aattcgatgc gaaattgaac aacgcggcct ttgtcgccaa
     2101 agcccccgaa gaggtgatcg acgagcagaa agagcgccgg gccgaagccg ccggcgttgc
     2161 ggggcgcctc agggaagctt tgaaacgatt gagcggggga ggggataatg cctgatcttc
     2221 tgctcgaact tttctccgaa gagattcccg cgcgcatgca ggtgcaggcg gcaaaggatt
     2281 tggaacgact cgtcgtcggc gcgctgtcgg atcgtggatt gctgttcgag gccgcgcgcg
     2341 gctttgccgg tccacggcgt ctgacgctgg cgattcaagg cttgccggtg aagcagccgg
     2401 acgtctcgga agaaaaaaaa ggcccgcgtg tcggcgcgcc cgaaaaggcc attgagggat
     2461 ttctgaaatc ggccggcgtt acgctcgacc aatgcgagac gcgcagcgac gtcaagggcg
     2521 aattctatct cgcggtgatc gcgcgcaagg gccgcgcgac taccgatgtg ctgagcgaaa
     2581 tcctgcccga acagatcgct aaactcccct ggccgaaaag catgcgctgg ggcgacggca
     2641 atttccgctg ggtgcggccg ctgcacggca tcgtggcgac gtttgaaggc gaggtggttc
     2701 cgtttgaagt tgcggacgtg aaaagcggca atgtcacgcg cggccatcgc tttctgtcat
     2761 ccggagaaat taccgttcgc catttcgagg attacgaaaa aaaactgcgc gatgcgcatg
     2821 tgatcctgga tggcgcggaa cggcgtgaga ttatcctgca tgcggcgcag caaaaggcgt
     2881 ttgcgcttgg actggaattg attcccgacg aggggctcgc gaatgaagtg atgggcctcg
     2941 ccgaatggcc ggtggtgctg atcgggcaaa tcgatcccga attcatggat gtgccgccag
     3001 agattttgca aacctccatg cgcacgcatc agaaatattt ttccttgcgc gatcccgcgc
     3061 gcggcaagct ggcgaaccgt ttcgtggcgg tcgcgaacat ggcggccgac gatggtggct
     3121 acgagatcgt tgccggcaat gagcgtgtgc tacgcgcgcg cctctcggac gcaaaattct
     3181 tctgggacca ggaccggaag cgcacgctcg aaagccgcgt tgacgatctg aaaaacatcg
     3241 tctttcacgc caagctcggc acgcaattgg agcgcgtaga ccgaatcgaa gccttggcag
     3301 gtgaaatcgc caagatcatc ggtgctgccc tcggcgaagc cgaacgaaat aagctcatcg
     3361 agaaggcgaa gcgcgccgcg cgtctgtgca aggccgatct gaccactggc gttgtgggcg
     3421 aattccccga actacaaggc gtcatgggcc gttactacgc gctgcattac gacgaggcgc
     3481 cggaagtcgc cgatgccatc cgcgatcatt acaagccgct cggaccatcc gatgctgttc
     3541 caaccgcgcc cgtctcaatc gctgtcgcat tggcggacaa gctcgacacg ctcgttggat
     3601 tcttcgcgat tgacgaaaag cccaccggct ctggcgatcc ttatgcgctt cgtagagcgg
     3661 gtctgggagt aattcgagtt ttatcaaatt gcacgttacg cattgagtta gtgcaactgc
     3721 tcgcttctca tgaagaccgc ataattaagt caattctaac tgttacagcg caggcggcac
     3781 taaagctccc agggaacaca ctattcccac cacatatcag agtttcgcca aggaatactg
     3841 cgctccagaa ttcaacccaa atatcattgg atattcttgc tttctttgcc gatcgcctca
     3901 aagttgcgtt gcgtgaaaaa ggtgtgcgcc acgatctgat cgatgcggtg ttcgcgcttg
     3961 ggtcagagga ggatctggtt cgtctcgtcc gccgtgtcga ggctttgcaa gcgttcctca
     4021 aaaccgacga cggcgccaat cttctcaccg gctatcgccg cgcggcgaac attctgaaaa
     4081 tcgaagagaa gaaggacaag cgcgactatc gcggcgtgcc ggatggtctg aagcaggatg
     4141 aggaaatcgc gctccaacgc gcgctgaccc acgccaaagt tgaaatggcc aaagcgctgg
     4201 ctgccgaaga tttcgccgat gccatgaggg cgctgtcctc cttgcgcgtg cctgtcgacc
     4261 acttcttcga caaggtcacc gtcaacaccg acgacaagac cttacgcgag aaccggctga
     4321 tgctgctgtc ccagatcgtc gagaccgcgc atcaggtggc cgatttctcc aaggtcgaag
     4381 gctaaacgca gcacctgtca tcgccgggcc atcctcgcga aagcaaggtc cggcgatcca
     4441 tgcttcctcc ccgatgaaag tgtttctgga tggcggcgct tcggccggcc atgacggcaa
     4501 gaggttaggg aaaacattct cggccggtct tgtccggata ttcttcgccc ccatgtctga
     4561 gggtcgtccg atagagaaca tttcatgatt ttcgtatggt taacgcaccc aattacacct
     4621 ctattcgcac cttattttcc gtcttctttt ggaagaaaag ccaagcaaat agccaaaaat
     4681 ttcctctcag cgccgatttt tcagggggga gggggtggca tcccggtggg ggccacgtca
     4741 caatgaaaaa tcagggatga aacgcccccg gccgggcagc tcaaccacac aaggaaaccg
     4801 gggcgaatga cgcagtgggt ctataatttt ggcgcgggcg ccgctgatgg gcgtgctgac
     4861 ctcagggcgc ttctgggcgg caagggggcg aacctcgccg agatgagcaa tctcggcctt
     4921 cccgtgccgc cgggcttcac catcacgacc gaggtctgca cctatttcta caaaaacggc
     4981 cagaactatc cggacgatct gaagcaagcc gttgaagcgg cgctgacaca ggtgggcaag
     5041 cttgccgatg gacgctttgg cgatgcggtg cgtcctctcc ttgtctcggt gcgctcaggc
     5101 gcgcgcgcct ccatgccggg catgatggat acggtgctca atctcggcct caaccccggc
     5161 acggtggagg ggcttgcccg gcaaaccggc gatgaacgct ttgcctatga ttcctatcgc
     5221 cgcttcattc agatgtattc gggcgtcgtg ctggaggtcg atcattctca tttcgaggag
     5281 atccttgaaa atgagaaaga ggccaaaggc atcgaacttg ataccgacct cgatgccgcg
     5341 gattggaagc gcatcgccgc actctataag gccaaggtca aacaggttct gggcaaaccg
     5401 tttcccgacg acgtgaacga gcaattgtgg tgcgccatcg gcgcggtgtt cggctcgtgg
     5461 atgggcgcgc gcgcgaaaac ctatcggcgg ctcaataaca tccctgaaga ttgggggacc
     5521 gccgtgaatg tgcaggcgat ggtgttcggc aatcgcggcc caacgtctgc gaccggcgtt
     5581 gcgttcacgc gcgatccttc gaccggcgag cgcgcgctgt acggcgaatt tctcatcaat
     5641 gcgcaaggcg aggacgtggt ggccggcatc cgcacgccgc aatccatcac caaacgcgcg
     5701 cgtgaggcga gccgatcgaa acgcgcatcc atggaagagg ccatgcccaa tgcgttcgtc
     5761 gaactctcgc ggatcgcaga tgcgcttgaa gcgcattacc gcgacatgca ggacgtggaa
     5821 ttcacggtgc aggaagacaa gctcttcatg ctccagacac ggtccggaaa acgcaccgca
     5881 aaggcggcgt tgcgtattgc caccgagatg gtgcgcgaac aacgcatcac ccaagacgaa
     5941 gcggtgatgc gtgtggatcc ctccgcgctc gatcaattgc tgcatccgac actcgatccg
     6001 aatgcgccca aggagctgat cgcttctggg cttgcggcat cgcctggagc ggcctcgggc
     6061 gaggcggtct ttaccgccga tgaagccgag cgcgcggcca gcgatggccg ggatgtaatt
     6121 ctgatacggc tcgaaaccag tccggaagac attcatggca tgcacgcggc caagggcatc
     6181 ctgacggtgc gcggcggcat gaccagtcac gccgctgtgg tcgcccgcgg catgggacgc
     6241 ccctgcgtaa caggtgcggg cacgctgaag atcgacgtag agcgcggcgc gatgagtacc
     6301 ggtacgatga ccatttcccg cggggacatc gttaccatcg acggtgcggc ggggaaggtt
     6361 tatcgcggcc gtgtaccgac gttgcaccct gaagtttctg gcgatttcgc cacgttgatg
     6421 gggtgggccg atgcgcggcg gcgcctgaag atccgtacca atgcggacac gccgtcggat
     6481 tgtgagacgg cgctgcgtta cggcgcggaa ggggtgggac tttgccgcac ggagcatatg
     6541 ttcttcgatg ccgagcgcat cctggcggtg cgccagatga ttctcgcgga tgacttggcg
     6601 ggccgcaaag ccgcgcttgc caaactcctt cccatgcaac ggggagattt ccacaagatt
     6661 tttcgcctga tggctgggct tccggttacc ataagattgc tcgatccgcc gctgcacgag
     6721 ttcattccgc attccgatga ggagatcgcc gaagtggcga aggcggctgg tagcgaaccc
     6781 gccaaattgc gcgctcgcgc gatggcgctc gccgaatcca atccgatgct cggccatcgc
     6841 ggctgccggt tggggatcac ctatccggaa atctacgaga tgcaggcgcg cgcgattttg
     6901 gaggccgcgt tggatgcgga aaacgaaaac ggcaagcccg tcgagctgga aatcatgatc
     6961 ccgctggtct ccttcaagag cgaactcgat tatctcaaag agaggatcga ggccgtagcg
     7021 caagccatta aaacggaatg cggcaaggcg ccttcctaca tgatcggcac catgatcgag
     7081 ctgccgcgtg ccgccctgat ggccaagcaa atcgctgaaa cagcggagtt tttcagcttc
     7141 ggcaccaatg accttacgca gacggcgttg ggcgtttcgc gcgatgacgc aggaagtttc
     7201 ctgagtgaat atctcgcacg cgggttcatc gaacgcgatc cgttcgtttc gatagatcgc
     7261 gacggcgtcg gtgaattggt cgagattgcg gtgaaacgcg gccggtccgc gcgtccgaaa
     7321 ttgaagctcg ggatttgcgg cgagcacggt ggcgaccccg actcgatcag gttctgccat
     7381 gaggtgggat tggactatgt ttcgtgttcg ccttttcgcg taccggtcgc ccgcctcgcg
     7441 gcagctcaag cagcgctcgc aaccgcgcga gaaaaagact gaacaatatg tgccgtccac
     7501 ggattatcct tctacggatg atcgtgggaa cctgacgccg cgtcatgatt taccatattc
     7561 gcgggttgac atggtcctta acccgctgtt agcaaagtat gtgaggtagg cccgcaatcg
     7621 tgcttgagaa ggatacagcc tggcatgatg aatggagtag gcaacgctca tcggcgcgca
     7681 gactgtgttg tggtgggtgc gatagctctt ctggcggtca gcagcgtttc agctcacgag
     7741 tttaatcttt atacgtatga attggaggcc cgcgcggcgc ttcccgatta cgttgcggtg
     7801 acgcagtcgc cggttgccct tgcgagagcc tacgcggcag cggaatccga ctgcttggcg
     7861 caggtgctct attatgaggc gcgcggcgaa ggtctcgatg gcgagaaggc ggtcgccgaa
     7921 gtcgttctcc aacgcacggt caatcgcgat taccccgaaa cggtgtgcgg cgtggcttat
     7981 gacggcgtgc agcccgaccg gcgcgattgc caattctctt ttgcctgcga cggctcgctg
     8041 cgcaaaccca aagacgcggt agcgtggacc cgcatccggc aattggcaga taaaatcatg
     8101 accggcgcga ttcggctttc cggtgcgacc gggcacgcaa tcgcctatca cagcgtggat
     8161 gtggcgccag cttgggcgga cagcatgctg aagaccgcga caattggaaa ccacgtcttt
     8221 tacaagcgcg atccgatggc gcagatgcgg cttgcgcaag cggtggcaaa gccgatcgaa
     8281 tcgctctcgg gcatgttcac cccggccatg acgacggggc cggcggtgcc ttcagaggaa
     8341 gtccagccct aggtccaaat tccgggcgct gtgggtaatg gcgcctgaag aaatgtagtc
     8401 gacgccggat tccgcgatgg cgcgcacgcg ctccagggta accccaccgg atgcctcaag
     8461 ccgggcacgg cctctggtga tcgcgacagc acgtttcatc gtttcgggcg gcatgttgtc
     8521 gagcaatatg gcgtccagcc caagagcaag cgcggattcg agctgttcca gcgtatcggc
     8581 ttcgacctcg atcctgacca tcggaccaag ccgtccgcgc aaggcgcgca ccacggcctc
     8641 gatgccgcct gccgctgcaa tatgattgtc tttcaccagt accgcatcat cgagaccgaa
     8701 gcgatgattg aagccgcctc cgcagcgcac cgcatatttc tccaaaatgc gcagcccggg
     8761 tgtcgttttg cgcgtacaga tgatccgcgc attggtgccg gccaccgctg agacgagtcg
     8821 atgtgttgcc gtcgcgacgc cggaaagatg gcctatgaaa ttcagcgcca cacgttcggc
     8881 ggtaaggatg gcgcgcgcag gtccctccaa cgcggcaaca atatcgccct cggccgcgct
     8941 gcttccgtcc ggaagattca cagtgaaaac gatggcagga tcgatcagac ggaacgtgat
     9001 gtcggcggcg ataaggccgc agatcacgcc tgtttctcgc gcggcgattt cagcgcgcgc
     9061 tatctggccc tgcgggatga ttgcatcggt ggtgatgtca ccagcgcgtc ccagatcttc
     9121 ttccaacgcc cggcgaacga gcggctcgat cagaagagca tgcggtggat tggtgtgggg
     9181 aagagcaagc cggttctcct gttgtgacag gccggccgcc atcacgccgc ctcgcgctgc
     9241 agaccgcgtg ctcgcggctt tactttcagc atggcttcca ccgcggcgcg cgcgcgggcc
     9301 gcgatcaaag gctctacgct gacttcggtc gtcatggttt ccagcgaatg gagaattttc
     9361 ggcagcgtga tccgcttcat gtgcggacag agattgcatg ggcgcacgaa ggatacgtcc
     9421 gggtattcca ccgcaacatt gtcgctcatg gaacattcgg tcatcagcac gacgcgcttg
     9481 ggacgcttct gaccgacata atcgatcatg cctgacgttg agcctgcgaa atcggcttcc
     9541 gctaccactt cgggaggaca ttccgggtgc gcgagtacaa tcaggccggg attggcgctg
     9601 cgcaagttgc gaacgtcctc tgcagtgaat cgctcatgca cctcgcaatg gcccttccag
     9661 gtgatgatct tgacggtagt ttgtttggcg acattctggg caaggaattc gtcggggatc
     9721 attatcacag aatcgacgcc ccattcggcg gcgatcgact ccacaacatc gaccgcattg
     9781 gaggatgtgc agcaaatgtc ggattcggct ttcacctccg ccgaagtgtt gacataggtg
     9841 atgatcggca aacccggata attctgccgc aagagacgca catcggcgcc attgatcgac
     9901 gcggcaaggg agcagcctgc ctccacgtcc ggaatgagca ccttcttgtg cggcgacagg
     9961 attttcgccg tctccgccat gaagtgtacg cctgcctgaa cgataatttt tgcatccgtc
    10021 ttggcagctt cgcgggcaag ttggagcgag tcgcccacga aatcggcgac gcaatggaag
    10081 atttctggtg tcatataatt gtgcgccaaa atcaccgcgt gccgctctcg tttcagtgcg
    10141 tgtatcgccg ccacgtaggg cgcaaatacg ggccactcga tttccggaac gacctgtttg
    10201 acgcgcgcgt aaaatggcgc tgtcgctgcg gcgatttcag gagtgtaagg caagtttttc
    10261 atgacgacat ttgtactcga tatgagcata tataggggct caaaaaaggc cggttccagg
    10321 ggacccgcca atgaccatta tgctactcat gagcattaca aaggtcaagc ctattcgttg
    10381 cgctaacgca gacggctgga tgttagacgc gcgccgggag tggggcgctc cctcaaaacc
    10441 tcgcgccgga aacggaattg ttcggccggg cggcctccgg ctttgcggga gaggcgtcct
    10501 gtgccttcaa ccaggccctg ggcttcgaca agccggcgga aattctgctt gtgcagtcga
    10561 atgccggaca aggcctccac agtccgttgg agttcgagca gggtgaattc cggcggcata
    10621 agctcgaaca cgactgggcg atacttcatc tttccgcgca gccgccccat ggcggttgcc
    10681 aaaatccgcc tgtgatcgaa caccatggct gaccctagag ggaggacggg cccaccggcc
    10741 ggcgctgcct gccggtcccg ctgggcctct ttcaccaagc ccgcttcgta aagaagctca
    10801 tagcgctcga gcaccttttc cccgtcccag gcaatgccgt cccccccaaa acaaagccgt
    10861 atccggtctt gacgcgaaac ccgcaagtcc ttggctgagg cgctgtccac gaactgcgcc
    10921 aaccggggca tgatcagagc gtcgatgatc ggcggttttt tctcgcgcca gtcctcccag
    10981 ggaaaatagc cataccaatc gtgccatagg gtttctggtc cgcgtccttg ctgaggccgc
    11041 gtcaacgcga gatagcccac ggacaatacg cgcggtcctt cgccgggccg ggtcatatgg
    11101 cggccgcgat cgccgaacgt gtaaagctgc tccacatagc caagctgcaa tcgggtctgt
    11161 tcctccaccc aattgcgcag acccgattca agggtgcgat gcgataaggg atcgaacgta
    11221 ccgaaaggca gcgcatcggc atcctcgccg cgatgcatca caagcacacg ggggcgctct
    11281 tccgccaccg agacgatcgc cgccgacaga ccgatcaata cggtttgagc ttcaccgggc
    11341 gtgacttcgt tattctttgc gtcgtcggtg aattttcgcg ccgatgccat cacagcagat
    11401 ccaagtcgag cggcagttcg acgatctcac tttgccagtt cggccgccct tcgcccatgg
    11461 cttcaacgcc cgcaatcatg cgtccttcgc ggccaaggag gcggtcggca tggcgaatca
    11521 tcagggggtt cggctgggcg tgaggagccc gaaaacgaag agccctcgca atgtggagtt
    11581 catgtccctg cccgtgaagg ttacacaaca cgatataggc cgcggcagta gagcggctga
    11641 ttcccgccca acaatgaacc aggaaaggcg ctctcctgtc ccaattcttg gcaaaagcaa
    11701 gcatttcggc gatgtgatgc ggctccggga caacgcttcc ctcagatggc tcgatgacat
    11761 cgtggacgcc gatgcgcaaa tggtggtcgg gatggatggc tttgggggtc tccacgaatg
    11821 gctcgatcat caaggtcacg agatgggacg ggcgatgccg gcgcacggtc tcttcgaggt
    11881 cggcgtaagg agcgatcagg atgtgcgaca tggtctcagt ctagcagcaa aatgcaggca
    11941 gctttcaagc cagcgctttg tgcggtttgg ccgagaaatg cgcttcaggc caccagtctg
    12001 tggaagcgct cgagatagag agtttgagca tcgactgctg gaacggggac caatcgcggt
    12061 ggcaccatgc cggacggggt gccgaagaat tttcgcgatt ccgtgaccgt gaaacccgcc
    12121 aattgaatgg cttcaaaata cgccgacatc cggtcagcac gtttgataac gccggattct
    12181 tccggcgtgt tttccggcgg caggccgaaa cgaatgtgaa tggcgcgtgc aaggcggttt
    12241 tcaaacgcct tgtagtcgag tccgacagcc atcttgaagg ggctgatcag gtcgccgatc
    12301 acatattccg ccgcgtcgtg cagcagcgcc gctaagcgca aagcgcgcga tgccttgggc
    12361 ttcagctcat gcacgaaacg ctccaccacc acacaatgct gcgccaccga gaacgcatgg
    12421 gcacccttgg tctgaccgtt ccatcgcgcc acgcgggcaa gcccatgcgc gatgtcgtcg
    12481 atctcgatgt cgagcggcga agggtccaaa agatccagcc tgcgtccgct cagcatgcgc
    12541 tgccaagcgc gggccgggtt ggctcggtcc ttcattcaga catctcttgt ttgcctctct
    12601 ctctttcccg tccgataata gccacccgac aaccgaggac aacatgccca ttgccaagat
    12661 ccttgctccc gtaacgggag gtgctcgtga tgccgctgtc ctcgctaatg cgttagaagc
    12721 ggcacgtccc ttcaatgccc acgtcatggc gttcttcgtg cggcctgatc ttgcagaagc
    12781 tcttgcattt ttcacggatg gaatctcggg agtggtggtc gaagaggtcg tgaaggccac
    12841 caaggatgca ggtgacgaag ccgccaggcg tgtggaagca gccatcgatg ccatatgcac
    12901 ggccaatggc atcacgcggg taacgcaacc ggctcgcagc aacgccgtca cgatgtcctt
    12961 gcgcgaagtt cagggcaatt tgggggattt gctgaccgcg gcggcccggc tttcagatct
    13021 ggtggtgttc gggcccattc gtgagaacga ccaggcaggt cttgcagaag cgttcgtgca
    13081 ggtgctggtc gaaaccgacc ggccggtgtt gcgcgcgacc gatgaggctc cgaaaagctt
    13141 cgcccggcgg attgccatcg gttgggacgg caagaccgct gccgcgcagg cggtcagtgc
    13201 ggccataccg tatttcacga aggccgacag cgtggagatc ctgagcatcc agaggcctcc
    13261 cttaaaggcc gaggccaccg aaggattgaa agaatatctc tctcttcatg gtgtccagag
    13321 caccgaacgg ctcatcgacc ccggcagcaa acgcattggc gaggtcttgc tcgatgcggc
    13381 gacgcaaggc gacgccgatc tcctcgtcgt cggtggttat ggccgcggcc gcttgcgcga
    13441 aagcctgatc ggaggcgtca cccgccatgt gatcgcccat gcgggactgt ccgtgttcat
    13501 ggtgcattaa ccggaaaaac ccaggttttg cctagaaaat aggcaatctt tggggctcgt
    13561 ctcaacacaa aattcagggt tttctgttat caacgaaggc gtccaaacac ggcgtctttc
    13621 gtgaatttta ctgatgtaaa cagaacgcgt gcaccgcaag aaatagtgac agtgggcacc
    13681 ctgtcatctc catcatcgac gcttctacat gggttggccc atatcgtcag tcgctggagc
    13741 tttacgctct ctctggcgct ggggttcgcg acagggctga cacttttgcc ggtagccggc
    13801 ggcgaattga ccggcgcgat catctccttc ggagcaatat gcattgctgc tgctgctgcc
    13861 cggcaagccc tcgaccgcaa gacccagata gagggtctcg aacaacaatt gctcgacgaa
    13921 acccgctacc acgcatttgt cgacgcggcc gcggaaggct tttttcgcac gacgcgcgag
    13981 ggccggtatg tgatcgcgaa cccggctttg gcacgtatct atgggtatga gtctccggag
    14041 cacttgtgcg ccgaactcac cgacatcggc cagacgctct atgtggatgt caaccgccgc
    14101 gcggaatttc agcgcctgat ttccgcaaat gcgcgggtaa aggattttgt ctctcaaatc
    14161 cgacagcggg acggaacctt gatttggatt tcggaaaacg ctcgtaccgt cagagacgag
    14221 gacgatcagt tccttttcta tgaaggcact gtcgaggaca tcacgtcgca gcgcgaatcc
    14281 gaagaggcaa cacgccgcgc cttggtcgaa acacaagaag ccgtgcgtgc caaggccgcc
    14341 tttctcgccg ccatgagcca tgagttgaag acgccgctca atgcggtaat cggattttcc
    14401 gatctaatgc gccaggaatt gttcggcccc gtaaacgaac cgcgctaccg cggctatatc
    14461 gcggatatcc acgacaatgg ccggcgtctc cttggcatga tcaacgacat tctcgatctg
    14521 tcgcggatcg agagccacct tctcgatctt gacgatgatt tggtctgcgt ccaggaggct
    14581 gtgagagtag cctacgccac gatcgtggaa ggcaaatctg acgcggcgcc gattgccttc
    14641 gaccttccgg cgcatgtgcc ttttttgaaa ggtgacccac ggcgtctaca tcaaattctc
    14701 gcgcatcttc tgtcgaacgc ggtgaagttt acgccgccta aaggccagat cacggtcaga
    14761 gtgtacctga cgaagggcgc cgaaatagcg atcatgattc gtgataccgg catcggcatg
    14821 gagccggggc ggatcgcgca cgcgctggaa ccgttcaagc aactcgacag cagcctttca
    14881 cgccggttcg aaggtgttgg gcttggtctt cctctcgcca atgccctggt acggctgcac
    14941 ggggggaccc tgacgatcga gagcgtgccc ggcgagggca ccattgtgac gatatgtttc
    15001 ccgccggagc gcagcgtcga aacggtacgc gccgccatcg cctgaacgtc tcgcacttag
    15061 gtcatctctg cgctacatct gggccatgag tgtgaaaaac cgaaaggcgt tggtgctgtt
    15121 ctcgggcggt caggattcga caacgtgtct tgcctgggcg ctcagtcaat tcgatcgcgt
    15181 cgaaacggta ggctttgcct atggtcaacg ccatgcggcc gaactcgacg ccaggcccaa
    15241 tattctgagg cgtatacgcg acgaattttt tcagtggcgc gacaggctgg gcgaggatca
    15301 tgtgcttgtg ctcgacgtct tgtcccagat agggggttcg gcgctcaccg atgaaattgc
    15361 cattgcaatg cggtccgatg gtcttccgac cactttcgtg ccgggacgta accttatgtt
    15421 cctcaccgcc gcggctgcgc tgggcacaag gcgggaagcc tacgatctgg tggggggggt
    15481 gtgcgaaacc gactattccg gttatcccga ttgccgtgac gaaaccatgc aggcgatgca
    15541 gagggcaatt acgcttggtt tcgcgcaggc cttcactatt cacacgccgc tgatgaaaat
    15601 cgacaaggct gaaacgtggc gcgtggccga acggctcggc ggcgaggttc ttgtgaacat
    15661 catcatcgaa gacacagtca cctgctacca gggcgatcgt tcccatcggc aggtttgggg
    15721 ctatggctgt ggggcgtgtc ccgcgtgcga tttgcgggcc gcgggctatc tgcgttatcg
    15781 cggggttgcc gcatgaccta tcaggtcaag gaaatttatt acacgctgca aggcgagggc
    15841 gcgcgcgccg gccgtgccgc tgtctttctc cgttttgccg gctgcaatct gtggagcgga
    15901 cgcgaggaag accgcgcagc ggcgatctgc aaattctgcg acacggattt tgtcggtacg
    15961 gatgggccgg gcggtggaaa atttgccgat gcggaatcgc tcgccaatgc ggttttgagc
    16021 aaatggccgg cggatcaggg aaaaccctat gtcgtatgta cgggcggcga gcctctgctc
    16081 caactcgatt ccgcattgat ccgtgcactg catgatgccg gcttcgaagt cgggatcgag
    16141 accaacggaa ccttgaaacc gcccaccggc atcgattgga tctgcgtcag tcccaaagcc
    16201 ggcgcagacc ttgctctcct tacgggcaac gagttgaagc ttatctatcc gcaaaccggc
    16261 gccgaaccgg aacgctacac cgggctggct tttgaacatt acttcctgca gcccatggac
    16321 agtccggagc gtgcccaaaa taccgccgcg gcaacgcaat attgcctggc gcatccgaag
    16381 tggcgcctca gtctgcaaac ccacaaactg attgggattc gatagggccc ttgacggcgg
    16441 gggcaggtgc actctaaggc ccggccatgt ccatgatcga gatcacgcag caattcggct
    16501 ttgacgccgc ccactatctg gaggacgccc cctcggcgtc caaccggcgc atgcatggcc
    16561 acagcttcta tgccgaggtc acccttcgtg gcatgccgga tcccgtaaag gggtgggtgc
    16621 gggatttcac cgagatcaac accgcgctgt ccgacattcg cgcgaggctg gatcatcaat
    16681 tgctgaacga tattgccggc ctcggcgcgc cgacgctgga aaatcttacg cgctatattt
    16741 tccgcgaggc gcgggcgctg atgcctgagg tctgccgcgt tcgtctgtcg cggccgtcct
    16801 cgggccaatc ttgtgttttc gaggagcccg gcagtgccgc gcaataagtc tccgctacag
    16861 ctcggccggc tcgtcgccgt tcccgcctca cccgaacagg cggtgctgga cgcggtatcg
    16921 aacccccatg ccgatacgga ctatgtggcg cgattcacgg cgccggaatt caccaccctg
    16981 tgtccgatca ccggtcagcc ggatttcgcg catttcgtga tcgactattg cccggcaaaa
    17041 agtcttctcg agtccaaatc cctgaagctg ttccttgcag cgttccgcaa tgttgccgga
    17101 tttcacgaag actgcaccgt caccataggc aaacgcatcg tgacggcgat ccgtccgaaa
    17161 tttctgcgca tcgcgggctt ctggtaccca cgaggtggaa tcccgatcga tgtgttctgg
    17221 cagcatggac gcctgccgaa aaacgtctgg ctgccggatc tcgaagtcga gccctatcgc
    17281 ggccggggct gagccgcgag cttcgcgcat gccgcgtggc gatgacagct gacgagatga
    17341 tcgttcacca tgccggtggc ctgtgcgaag gcataaacga tcacgggccc gcaaaatttg
    17401 aagccccggc ctttcaattc tttggccagg cgctcgctca tctcggtctt ggcgggaatg
    17461 tcctctatcc gcttcaagcg attggtgcgg ggcttgccgt ccacatgttt ccaaatcaga
    17521 tcggaaaatc ctccaggctc tttttccatg atatcgagcc aaagtcgcgc gccagtgatg
    17581 gccgcctcga tcttcaggcg gcttcggaca atggaggcgt cctccagcag gcgcttcatg
    17641 tccttggcgc cgtagcgcgc tattttctcg ggcttgaagc cctggaaggc gcggcgaaag
    17701 tcttctcgtt tgcgcaaaat agtgatccag gcgaggcccg cctggaatcc gtcgagtacc
    17761 agcttctcgt aaagcgcgcg gtcgtcgaat tccggcacac cccactcttc atcgtgatag
    17821 gcgcgataga gcgggtcttc gcccggccac ggacagcgcg attgctcaga catgaagcca
    17881 ctccggtttg ttcaattgga cctcccggcc gtccgccgtg accggcgcgc gttttgcttc
    17941 ggcttccgcc cagcgatcga gacgcacgag cgcaagcgct cttccgttcg cgccggtcgc
    18001 aaacgtggcc aactcgcgtt cctccgatat gatcggcgtg ccgggcgcgg gcgcctcgcc
    18061 ggcaaattcc gccacgaaga aacgacgcct tgccgctgcg cgatgcttca tgcgggcggt
    18121 cacctcctgt ccgataaagc agcccttctt gaagctgacg ccattgagtt cttcgagccc
    18181 ggcatcgagg gcaaatacgg aatcgggcgt caggtcggca ctgtcgggaa cgccgagcac
    18241 aaggcggtgg agctgatagt cgcttgccgc gagaccgcca atcgcgtttg caagcgcgtt
    18301 acgcgcgccg atcatccgca ccccaagcga tggatgccgg ggatcgcgga aacgcaccac
    18361 atcgccgggg atttcgaatg ccccgttatc gtcccaagcc gcggccaaag cgagatcggt
    18421 tgggtgggcg atcttcacct tcgcgcggag tttgtaaagc gtgagccgct tcatcagatc
    18481 ggtggcacgc gaggccgcgc aatcgatcag cagccggccc tcagcgtcag gaacgacgaa
    18541 aaagtcgaac aggattttgc cttgcggtgt cagcagcgcc gtatagaccg gttgttcttc
    18601 ggcgacagtg gtcacatcat tggtcgttag cccttgcagg aaatcgcgcg cttcggggcc
    18661 gccgtgtgca agaacggcgc gatcatcgag aaatgatgca tggatcatgg tttctcagtt
    18721 agtgcgccgc gctcaccgag gcaagtggtt gccaagttcc gatgggctgt cgtacttctg
    18781 acgtcccctt gaagaaggtt ccatggcgct gaaaatcctc accgtgctgg cgggagcatc
    18841 cgtaggcggc gccgaaacat ttttcgtcag cctgacgctt gccctggcgc acgccggggc
    18901 gg
//
LOCUS       DASYEG010000165         3323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1649270, whole genome shotgun sequence.
ACCESSION   DASYEG010000165 DASYEG010000000
VERSION     DASYEG010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649270"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VGM72_07525"
     CDS             complement(<1..322)
                     /locus_tag="VGM72_07525"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEY4265150.1"
                     /translation="MAMMRFLATMTGLVLTLGAPAGAFAQSGALPDDKPKVNASLVAE
                     RAGVAPGGTVTLIVHETIRKGWHTYWRNPGDAGFPTTAKWQLPEGWKAGDIQWPYPKR
                     LPLEQ"
     gene            445..1026
                     /gene="rsmD"
                     /locus_tag="VGM72_07530"
     CDS             445..1026
                     /gene="rsmD"
                     /locus_tag="VGM72_07530"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEY4265151.1"
                     /translation="MRITAGKFKGRALATPGDQSVRPTSDKVRQAVFNILEHRDFGFP
                     FALEDARIVDLFAGTGALGLEALSRGARYALFVDDAAGSRALIRESVEAFGLTGASKI
                     WRRDASALGPLDTLGAFDLAFLDPPYRKGLIAPALAGLRDGGWLNPPALVVAEMAEDE
                     NVPPTDGYETLDDRVYGDTRVAILKFSLRALKD"
     gene            complement(1057..2895)
                     /gene="mutL"
                     /locus_tag="VGM72_07535"
     CDS             complement(1057..2895)
                     /gene="mutL"
                     /locus_tag="VGM72_07535"
                     /inference="COORDINATES: protein motif:HMM:NF000953.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEY4265152.1"
                     /translation="MGIETKPVRAIRRLAEGTINRIAAGEVVERPAAAVKELAENALD
                     AGARRIAVTVANGGASLILVEDDGEGMDAGGLRLAVERHATSKLPIAADGADDLTNIA
                     TLGFRGEALPSIGAVARLSITSRTKAGDAHAIRVEGGTVGGPEPAGFLSAGESGTRVE
                     VRDLFYATPARLKFLKSSRSESLAVIDVVKRLAMARPDVAFSLTDEGRQVLSLAAEER
                     LFEGVLARLAKLMGRDFADNAARVEASRESVRLSGFAAVPTYNRANALAQFLFVNGRP
                     VRDRVLLGAVKGAYADFLARDRHPAVALFLDCDPEFVDVNVHPAKAEVRFRDSGLVRG
                     LIVGALKAALHEASHRASTTVADAALGAFRASPMWPSERLPMQPHLSGLSDSVRNYQA
                     PLAAAVPDAARAPMIAPHEHGQDHADDTPPLGVARAQLHETYIVAQTKDGIVIVDQHA
                     AHERLVYERMKQSLAGGGVARQPLLIPEIVEMDESEAARILARSEDLAALGLVLEGFG
                     PGALMVRDVPALLGQVDVKALIRDLADDLAESESVRSLKERLDHISATLACHMSVRSG
                     RRLNVEEMNALLREMETTPHSGQCNHGRPTYVELKLADIERLFGRR"
BASE COUNT          591 a         1095 c         1059 g          578 t
ORIGIN      
        1 actgttcgag cggcaggcgc ttgggatagg gccattggat gtcccccgcc ttccagcctt
       61 ccggaagctg ccatttcgcg gtggtgggaa aaccggcgtc gcccgggttg cgccaatagg
      121 tatgccagcc tttgcggata gtctcatgca cgatcagcgt gaccgtgccg ccgggcgcaa
      181 cccccgcacg ttcggcaacg aggctcgcat tcaccttggg cttgtcgtcg ggcagcgcgc
      241 ccgattgggc aaaagcgccc gccggcgcgc cgagcgtcag aaccaagccg gtcatggtcg
      301 cgagaaaccg catcatggcc atagctatac tcttccgggg gctgccgaaa aagcaccggc
      361 cctgaggctc gcggggtcgt gaggcgcgag gcggcggttc cctgccgcta ggcaaacgcg
      421 cgcaaacgcc ggtaagaagc ggcgatgcgc atcacggcag gcaaattcaa ggggcgcgcg
      481 ctggccacgc ccggagacca aagtgtcagg ccaacctcgg acaaggtccg ccaggccgtg
      541 ttcaacatcc tggaacaccg cgatttcggc tttcccttcg cgcttgagga cgcccgaatc
      601 gtggatcttt tcgcgggcac aggcgcactt ggactcgaag cgctgtcgcg gggtgcacgc
      661 tatgctctgt tcgtggacga tgcggccggg tcgcgggcgc tgatccgcga gagtgtcgag
      721 gctttcgggc tgaccggcgc aagcaagatc tggcggcggg atgccagcgc gctcggaccg
      781 ctcgacacgc ttggcgcttt cgatcttgcc ttcctcgatc cgccgtaccg caaggggctc
      841 atcgcgcccg cgcttgccgg tttgcgcgat ggcggctggc tgaacccgcc ggccctcgtg
      901 gtcgccgaaa tggcggagga cgagaatgtt ccgcctacgg atggatacga gactttggat
      961 gaccgcgtct acggcgatac gcgcgtggcg atcctgaaat tttctctccg tgccctcaaa
     1021 gattagccgc acggccttgg ccccgctccc tggaatctag cgccgcccga agaggcgctc
     1081 gatgtcggcg agcttcaact ccacataggt cggacggccg tggttgcact gccccgaatg
     1141 gggcgtcgtt tccatctcgc gcagcagcgc gttcatttct tccacgttca gacgccggcc
     1201 cgaccgcacg ctcatatggc aggcgagcgt ggcggagatg tgatccagcc gttctttcag
     1261 cgatcgcacg gattcggact cggcgaggtc atcggccaaa tcgcggatca gcgctttgac
     1321 atccacctgc cccaacaatg ccggtacatc gcgcaccatc agcgcgccgg ggccaaaacc
     1381 ctcgagcaca agtcccagag cggcaaggtc ttcgctgcgc gccagaatgc gggccgcttc
     1441 gctctcgtcc atctccacga tctcggggat cagcagcggc tggcgcgcga cgccgccacc
     1501 ggcgagcgat tgtttcatgc gttcgtagac cagccgctcg tgagcggcat gctgatcgac
     1561 gatcacgatg ccatccttgg tctgcgcgac gatgtaagtc tcatgcaatt gcgcccgcgc
     1621 aacaccgagc ggcggcgtgt catcggcgtg atcctgccca tgttcatgtg gcgcgatcat
     1681 gggcgcacgg gccgcatccg gcacggcggc agcgaggggc gcctgataat tgcgtaccga
     1741 atcgctcaag cccgaaagat ggggctgcat gggtagcctt tcgctcggcc acatcgggga
     1801 tgcgcgaaag gcacccagtg ccgcatcggc gacagtggtg gaagcgcgat ggctcgcctc
     1861 gtgaagcgcg gccttcagcg cgcccacgat cagcccgcgt acgaggcccg aatcgcgaaa
     1921 ccgcacttcg gctttggccg gatgcacgtt cacgtccacg aattccggat cgcaatcgag
     1981 gaacagcgcc accgccggat ggcggtcgcg cgccaggaaa tcggcatagg cgcccttgac
     2041 cgcgccgagc agcaccctgt cgcggacggg ccgcccgttg acgaacagga actgggccag
     2101 cgcattggcg cgattgtagg tcggaacggc ggcgaaaccg gagagacgca cggattcgcg
     2161 tgacgcctcg acgcgcgccg cattgtccgc gaaatcgcgg cccatcaatt tggcgagccg
     2221 cgccagcaca ccttcgaaca ggcgttcctc cgcggccagc gacagaacct ggcgcccctc
     2281 atcggtcagg ctgaacgcca cgtcgggacg cgccatggcg agacgcttga ccacatcgat
     2341 cacggcgagg gattccgacc gcgacgattt caggaatttc agccgcgccg gcgtcgcata
     2401 gaagagatcg cgcacctcga cccgcgtgcc ggattcgccc gcactgagga atccggccgg
     2461 ttcgggaccg ccgaccgtcc ctccctccac gcgaatcgca tgggcatcgc cggcctttgt
     2521 gcggctggtg atggaaagcc tcgccaccgc gccgatggaa ggcagagcct cgccgcgaaa
     2581 gcccagcgtc gcgatgttgg tgagatcgtc cgcgccatcc gcggcaatcg gcagtttcga
     2641 ggtggcgtgc cgctcgaccg cgaggcgaag cccccccgca tccatgcctt caccgtcatc
     2701 ctcgacgagg atcagcgagg cgcccccgtt cgcaaccgtc accgcgatgc ggcgggcacc
     2761 ggcatcgagc gcattttcgg ccagctcctt caccgcggcg gccggacgct cgaccacttc
     2821 accggccgcg atgcgattga tcgtgccttc ggcgagacgg cgaatggcgc gcactggctt
     2881 ggtctcaatc cccatggcga cccttgatcg actcggcgga gattgtagcg cagttccgaa
     2941 acaaggtgca gttttggtcc ggcggcccgt ggcatccttc cacaggacaa gagagtccgt
     3001 cagggcgcgc cgtgggcaat caagagggac acgaaggcca caaaattaac acgaagagat
     3061 ttttgtgctc tttgtgacct tcgtgtcctc cgttatttcc cacaagcaaa tggcgccgcc
     3121 acttgcgcag tggcgccatt tggccggctt ttatcagcct aatttatttt ccgattggcg
     3181 gcaggccgct cacatatttg aagcgctgca cgcgctggcc ctgcacttcg gcagagtaga
     3241 tgttgccctt gctgtcgatg gcgatgttgt gaagattctc gaactcgccg ggaccgcggc
     3301 cgacacggcc gaaactaccg agg
//
LOCUS       DASYEG010000166         6563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1501398, whole genome shotgun sequence.
ACCESSION   DASYEG010000166 DASYEG010000000
VERSION     DASYEG010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6563
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501398"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..811
                     /locus_tag="VGM72_07540"
     CDS             44..811
                     /locus_tag="VGM72_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4265153.1"
                     /translation="MAQRIGKRLPTLLHRSVIAMRGQPMVSGSIQHSAAEISIGGGMQ
                     ILIISSVRLLRDGLAAMLQQRANVQGAEVAESAEAAIEALERSAPTLILLDVAADDGL
                     LVARRLAEAAPHVHILGFAARAYDHDVVAYASAGITGFVPREASAQDLFDAIDRAVNG
                     ELLCSPRVAATLFRQLAVLSNTPKHGVGPTQLTGREREIVRFIDEGLSNKEISRHLSI
                     EVSTVKNHVHNILEKLNVTCRGKVPARVRVLETSHPD"
     gene            1125..1451
                     /locus_tag="VGM72_07545"
     CDS             1125..1451
                     /locus_tag="VGM72_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265154.1"
                     /translation="MNVSRLLGDVMGGIVAGDSGIELVSGVSTQDMLDAIDAHNPNVV
                     VLGSEAGAIDAHLALIAANHPTLRVISVDVDGRSATIYEPGAVERRVTDISSRMMLSL
                     IRGSVG"
     gene            1501..3657
                     /locus_tag="VGM72_07550"
     CDS             1501..3657
                     /locus_tag="VGM72_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY4265155.1"
                     /translation="MSSGSDAKKSVNFQTVVVHHAHTTLAAALRALDEILTIAVDRQA
                     RLLGPASLLDPWRGMHLEQADIRRLLDSASNAELFGSGDPTVSDWLAAALRTSPRIES
                     IARKLRLSNIDLAILLIVLAPDLDLRYERIYGYLQDDIARKRPTQDLIANLLAQGPGE
                     RLAVCARFGERAPLLAFDLLVTHDLMDAPPLAHAWLVDELWRRWLLGDTDIGSYIGGM
                     ARLFMPPPLGLDQIPLEEPMRQRVRRAIDAKTGQALRLRLLMHGPHGSGKFALAQAIG
                     FELKQRLLVIDLRDCGQVAELRTLTQRIQRGAMLSGALLYFHGFSQFEHRDPQLLRVL
                     FEALGHSPCGFILSCATRLPQLHAGVLPVMRVELAYPSPDVRLQAWRQALAAYDLQVE
                     ETSIEHIATRFALSAAQIQQAVAETHVSAVTSKTGEIGYDDLACAVRMQCGTELSRMA
                     THIVPRAAFESLVVPEEVQAQLREICMRVATRDTVGREWAGGSVHARSVGVTALFVGP
                     SGTGKTLSAEALAHELGLHLYCIDLSGVVSKYIGETEKNLDRVFAAAEHANAVLFFDE
                     ADALFGKRSEVKDAHDRYANIEVAYLLQKMERFDGLAILATNLRQNLDESFARRLTFT
                     VNFPFPEEPERRRLWEQLWPPRAPRHADVDLTWFAHEFKLSGGNIRNTVLAAAHLAAA
                     DGKIITHQHLLHATRREFQKLGKNLVQPATGERRAG"
     gene            complement(3792..4019)
                     /locus_tag="VGM72_07555"
     CDS             complement(3792..4019)
                     /locus_tag="VGM72_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265156.1"
                     /translation="MSQFMRQQLLALGGVRSVAVLAENDIGAEGIGLRSGFRSAAMRL
                     IIVMDTDILKTRVEAGLHVAAYTRVKRGAAD"
     gene            4161..5879
                     /locus_tag="VGM72_07560"
     CDS             4161..5879
                     /locus_tag="VGM72_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265157.1"
                     /translation="MHDKDDRRKRGGKGGSGDDGGGRDKKAERDRAKRETQERKQQRA
                     SAGKDDQQIREQRAEKKLRSLERDAGAPGAKSRSLASKNKKLKNFEDVLKDAGGNQGK
                     KNVRKGKMDELQRTPRDVAGNPQEKFVGGGREVPGQELRPGQEHYAQPDYSLYRRHPD
                     GTAERLHVNLKSDKLYQQSPAKARATARNNTAQAIKNSRHLPNGETIIISYAHTPDPE
                     IQAIMLEEHFGSGSPISEVRFGTTTHSRSTYKPPAKPPAKPKASKGKAKAKKPAQKKG
                     AKKAAKAKPAQKTQKKAPSKAKAKAKAKGKAKPTKKSKPKDTSKAQDKVEGKGKAGSK
                     GAGDATTKSGTEGATAKVEKQTVTDKPATSTPKPDAKPAPSGTPPINDAAPAKPVVEA
                     KAVEPPKPPTITERGVVPEVGPKGRGPAIGPFEVLGILGAIPAAKDIRNDIKEGHYGT
                     ATAKAALLRLSFVAEAAPPLFSAAVISTYWGERHEKIQEDSFAAGEAVENLVERIPVV
                     NKVPYLPKVLGALKAADTAVGESIAYTVKDMGEAIGEGAEDVYDWLTEPMVSDEEMKQ
                     ILKQFE"
     gene            5882..>6563
                     /locus_tag="VGM72_07565"
     CDS             5882..>6563
                     /locus_tag="VGM72_07565"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEY4265158.1"
                     /translation="MPDGAQKTASKANTAAPERRPLRSIAVSAEAAPALDAMRALHGQ
                     AGNRAVTQAIGPGIPLAPELRTEMESRFGADFHDVRIHNDRQAHASAALMDAKAYTQG
                     RDIVFGMDRFAPHAGAGRRLLAHELAHVVQQRGGGAAPALDAHGPHERDAARAADAYA
                     GGEGAVTVAASTGPGVAREEDDDSWGARFTRRVNALKERIPERYREPIAKVADVVADK
                     TVSVVLSPI"
BASE COUNT         1409 a         1996 c         1994 g         1164 t
ORIGIN      
        1 ggctgcaatg tccgccttgc ttgttttata gtaccagcag gtggtggcac agcggatcgg
       61 caaacgcttg cctacgctct tgcatcgttc ggtgatcgcc atgagggggc aaccgatggt
      121 gtcgggttca attcaacaca gtgcggccga gatttccatc ggcggcggaa tgcagatcct
      181 gatcataagc tcggtccgtc tgcttcggga cggtctcgca gcgatgctcc agcagcgcgc
      241 caatgtgcaa ggtgcagaag tcgccgaaag tgcggaagcc gccatcgagg cgctggaacg
      301 gtcggcgcca acactcatcc tcctggatgt cgccgccgat gacggtctgc tggtcgcccg
      361 tcgattggcg gaggcagcgc ctcacgttca catattgggg tttgctgccc gcgcatatga
      421 tcacgacgtt gtggcctatg ccagcgcggg catcacgggt tttgtccctc gcgaagcatc
      481 cgcccaggat ctatttgacg ctatcgaccg cgccgtcaat ggcgagctac tctgctctcc
      541 gcgtgttgcc gcgacattgt ttcggcagct cgccgtcctg tccaacacgc cgaaacacgg
      601 cgtcggcccg acgcaattga ccggccggga gcgcgaaatc gtccgtttca tcgatgaggg
      661 gctttccaac aaggaaatat cccggcacct gtcgatcgaa gtctcgactg tcaagaatca
      721 cgtccacaac atcctcgaga aactcaacgt aacctgccgc ggcaaggtcc cggcgcgtgt
      781 ccgagttctg gagacctccc accccgacta ggtccatcga tgcgtttgga tcatagatcc
      841 atcggttcag ggctggatcg aaggatccgg tcaatccgct ctcgcgcgat gcaacgatac
      901 ttcgctcgcc agccacacac atgcgcgttg atggttttaa gaggccgaca gccgcctcca
      961 cacgccaatg gctcgtggcc aacaaatcca tggcggcgga ccatcggata cagatccatc
     1021 gaccatcgga aaccagccgc ctgatctatt ccacgtccag tgaatgacgg acatgatccc
     1081 agccgatgct ggagcggcat ttgacccatt tgcgagttct tgtcgtgaat gtttcccgcc
     1141 ttctcggcga cgtgatgggc ggcatcgtag ccggtgactc tgggatcgaa ctcgtcagcg
     1201 gagtaagcac gcaggatatg cttgacgcga tcgacgccca taatcccaac gtggtcgttc
     1261 tgggtagcga agccggcgcc atcgatgcgc atctcgcgct tatcgccgcc aatcatccga
     1321 cgctgcgcgt catctccgtc gatgtcgatg ggcggtcagc aaccatttac gagccgggcg
     1381 ctgttgagag acgggtgacc gacatatcgt cgcgcatgat gctgagcctg atccgtggct
     1441 cggttggata gtccacgtgt caatcatgat cgcattgcgg atgaaaggga atgtagagca
     1501 atgagctccg gctcagacgc caagaaatcg gtgaacttcc agacagtcgt tgtccaccac
     1561 gcgcacacca cattggccgc cgcgctgagg gcattggacg aaatcctcac gatcgccgtg
     1621 gatcggcaag cgcgccttct gggcccagcc tctctcctgg atccgtggcg cggcatgcac
     1681 ctggaacagg cggatatacg ccgtttgctg gactctgctt ccaatgccga gctttttgga
     1741 tccggcgatc ccacggtttc cgattggctt gccgcggccc tgcgcacgtc tcctcgcatc
     1801 gaatcgatcg cgcgtaagct acgcctgtcg aatatcgatc ttgccatcct tctcattgtg
     1861 ctcgcgccgg atttggatct gcgatacgag cgaatctacg ggtatttaca agatgacatc
     1921 gcacgcaagc gcccgacgca ggatctgatc gccaatctat tggcgcaggg ccccggtgaa
     1981 cgcctggctg tttgcgcacg cttcggcgaa agagcccccc tgctcgcctt cgacttgctt
     2041 gttacccatg atctcatgga tgcaccgccg ttggcgcatg cctggctcgt ggacgaattg
     2101 tggcggagat ggcttctcgg cgacacggac attggatctt acataggcgg catggcccgc
     2161 ctattcatgc cgccccctct ggggttggac cagattccgc tggaagagcc gatgcggcaa
     2221 cgggtgcggc gggcgatcga cgctaagacc ggccaagcac tccgtttgcg tcttctgatg
     2281 catggacctc acggaagcgg gaaattcgcc ctggcacagg cgatcggctt cgaactcaag
     2341 caacggcttc tggtgatcga tctgcgcgac tgcggccagg tcgcggaact ccgcacgctc
     2401 acacaaagga tacagcgcgg cgccatgctg tcgggcgctc tgctctattt tcacggcttt
     2461 tcacaattcg aacaccgcga cccgcaacta ctccgggtgc tgttcgaggc gctgggccat
     2521 tcgccctgcg gcttcattct gtcctgcgca acccggcttc ctcagcttca cgccggtgtt
     2581 ctgcccgtga tgcgcgtcga actcgcctac ccctctccag acgtgcgact gcaagcatgg
     2641 cgccaggctc tggccgccta tgatctgcaa gtcgaagaaa ccagcattga gcatattgcg
     2701 acgcgctttg cgctgagcgc ggcccagatc caacaggccg tggccgagac gcatgtctct
     2761 gccgttacat cgaagaccgg cgagatcggc tatgacgacc tcgcttgtgc cgtccggatg
     2821 caatgcggaa ccgaactgtc gcgcatggcg actcatatcg tgccgcgcgc cgctttcgaa
     2881 tcactggtgg tcccggagga agtgcaggcc cagcttcggg agatctgcat gcgcgtggcg
     2941 acgcgggaca ccgtcggccg cgaatgggcg ggaggcagcg tgcatgcccg cagtgtcggc
     3001 gtcaccgcgc ttttcgtggg tccttccggt acgggaaaga cgttgtcggc ggaggccctg
     3061 gcgcatgagc ttggccttca tctgtattgc atcgatctct ccggcgtcgt cagcaaatac
     3121 atcggcgaaa cggagaagaa tctcgatcgc gtgttcgctg ccgccgaaca tgccaacgcg
     3181 gtgctgtttt tcgatgaagc cgatgcgctg ttcggcaaac gatccgaggt caaggatgct
     3241 catgatcgct atgccaacat cgaagtcgct tatctcctgc agaaaatgga gcggttcgac
     3301 gggctggcga tcctggctac caatctcagg cagaatctcg acgagtcatt tgcccgccgc
     3361 ctgacattca ccgtcaattt tccatttccg gaagaacccg agcggcgccg gctctgggaa
     3421 cagttgtggc cgccgcgcgc gccgcgccac gcggatgtcg acctcacctg gttcgcgcac
     3481 gagttcaaat tatccggtgg aaacattcga aacacggtgt tagccgccgc acacctcgct
     3541 gccgcggatg gaaaaatcat cacgcaccag catctcctgc acgccacgcg ccgcgaattt
     3601 cagaagcttg gcaagaactt ggttcagccg gccacgggtg aaaggcgggc cggatgacct
     3661 ttggatcggg tcgttgcaag ataggagaat catccgcaca gccggccaag cgggacgagc
     3721 attccaaacg cactctcgga acggatgcca cgttggaacg agccacttcc aaccgtggac
     3781 tctccagtgc tttaatcggc ggcaccccgc ttgaccctcg tatacgcagc gacatggaga
     3841 cccgcttcaa cgcgcgtttt gaggatgtcc gtatccatga cgatgatcag gcgcatcgca
     3901 gcgctgcgga atccggatcg aaggcctatg ccttcggcac cgatatcgtt ttcggcgaga
     3961 accgctacgc tccgcacacc gccgagggca agcaattgct ggcgcatgaa ctggctcatg
     4021 tcgttcagca acgtcgtggc ggagatgtgc ccggcgaagg aacccgcccc gctttggaaa
     4081 aggccgcttc ggccgccgca aattctgtcg cggcaggagc cgcgcaggtt ccggttggcg
     4141 gcgcctcttc cgtcggcgtc atgcacgaca aggacgaccg ccggaagaga ggcggaaaag
     4201 ggggatccgg cgacgatggc ggcggccgcg ataagaaagc agagcgcgat cgggccaagc
     4261 gggagacgca ggagcgcaag cagcaaaggg cgtccgccgg caaggacgac caacagataa
     4321 gggagcagcg cgccgagaaa aagctgcggt cgctggaaag ggatgccgga gctccgggcg
     4381 cgaagagcag atcactggcc agcaagaaca agaagctcaa gaattttgaa gatgtgctga
     4441 aggacgccgg aggcaaccag ggcaaaaaga atgtgcgcaa agggaagatg gatgagttgc
     4501 agcgcacgcc tagggatgtc gccggtaatc cacaggaaaa atttgtggga ggcggacgtg
     4561 aagtccccgg ccaggagtta cgtcccggcc aggagcacta tgcccagccc gactattctc
     4621 tctatcgccg ccatccggac ggcacggcgg agcgcctcca cgtcaatttg aaatccgaca
     4681 agctctatca gcagagcccg gccaaggcgc gggccacggc gcgaaacaac accgcccagg
     4741 caatcaagaa ctcgcgacat ctcccgaatg gcgaaaccat catcatcagc tatgcccata
     4801 ctccagatcc ggagattcag gccattatgc tcgaagagca tttcggttct gggagtccga
     4861 tcagcgaggt gaggttcggg accacgacgc atagccgcag cacttacaaa ccgcctgcca
     4921 aacctcccgc caaacccaaa gcgtcaaagg ggaaggccaa ggccaagaag ccggcgcaga
     4981 aaaagggtgc caagaaagcc gccaaggcaa aacccgcgca aaagactcaa aaaaaggcac
     5041 cgtcgaaggc gaaggcaaag gcaaaggcaa agggcaaagc caagccaacc aagaagtcga
     5101 agccgaagga tacttcaaag gcccaggaca aagttgaagg taagggcaag gctggcagca
     5161 agggagccgg agacgcgacg acgaaatcgg gcaccgaggg cgccaccgcc aaagtcgaaa
     5221 agcaaaccgt caccgacaaa cccgcgacgt ccacaccgaa gcctgacgca aaacccgcgc
     5281 cttcgggaac accgcccatt aatgacgccg caccggccaa gccagtcgtc gaggccaagg
     5341 cggtcgagcc gccgaaaccg ccgacgatca ccgaacgtgg cgtggtgccg gaggtaggcc
     5401 ccaagggacg cggccccgcg atcgggccct tcgaagtgct cgggatttta ggagcgattc
     5461 ccgcggccaa ggacattcgc aacgatatca aggagggtca ttacggcacg gccacagcca
     5521 aagccgcatt gctgcggctt tcattcgtcg cagaagcggc tccgccgctg ttctccgcgg
     5581 cggtgatctc cacatattgg ggcgagcggc acgagaagat ccaggaagat tcattcgccg
     5641 ccggtgaggc cgtggagaat ctcgtggagc gcatccccgt cgtaaacaaa gtcccatacc
     5701 tgccgaaagt cttgggagcg cttaaggcgg ccgataccgc cgtcggcgaa tccatcgcct
     5761 acactgtcaa ggatatgggg gaagcgatcg gagaaggcgc cgaggatgtc tatgactggc
     5821 tgaccgagcc gatggtgagt gatgaagaaa tgaagcagat tttgaagcag ttcgaataga
     5881 catgccggac ggtgcgcaga aaaccgcgag caaggcaaac actgccgccc cggagcggcg
     5941 accgttacgc tccatcgcgg tgtccgccga agccgctcca gcgctcgatg cgatgcgagc
     6001 attgcatggc caagcgggaa accgcgccgt cacgcaagcg attggtcccg ggataccgct
     6061 cgcgccggaa ctccggactg aaatggaatc ccggttcggg gccgattttc acgatgtgcg
     6121 cattcacaat gatcgccaag cgcatgccag tgcggcgctg atggacgcaa aagcctacac
     6181 gcagggccgt gacatcgttt tcggcatgga tcgcttcgcc cctcatgcgg gcgccggcag
     6241 gcgtttgttg gcgcatgaat tggcacatgt cgtgcagcag cgcggcggcg gggcagcgcc
     6301 cgcactggat gcgcacggtc cgcatgaacg agacgccgcg cgcgccgcag atgcctatgc
     6361 cggcggcgaa ggtgcggtca ccgtagcggc ctcgacaggg cctggcgtcg cacgcgaaga
     6421 agacgatgac agctggggcg cacgctttac gcgccgtgtc aacgcgttga aggagagaat
     6481 tcccgagaga taccgcgaac cgattgccaa agtcgccgat gtcgtggcgg ataagaccgt
     6541 cagcgtggtg ctgagtccta tcg
//
LOCUS       DASYEG010000167        10254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_632505, whole genome shotgun sequence.
ACCESSION   DASYEG010000167 DASYEG010000000
VERSION     DASYEG010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 10254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10254
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632505"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1936
                     /locus_tag="VGM72_07570"
     CDS             <1..1936
                     /locus_tag="VGM72_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265159.1"
                     /translation="KPCCLFYTTSLSGGVETWSSRIDIADYGLGSDGLGTWFYSSLMY
                     VANNTELHFVATKAKSDNSIRQDVHYFIYDTTDGSLKNFDKSFSTAAGSLPVLIADLD
                     AHYRIFAQPTAGGSTGIPKFLFDAAGRANVLTIDGTPGSGDFDVWHLILQSGSWTDTM
                     IGNTNSEVNDYALIPAVAGGVTAYFPQAATAGFAVDGDYYTTTRDASTGTWSPVELFL
                     ASDGINALAVGLPVLNGIAALRFVFSEIHQSSADSGAGGLRKYAYGDFGYVTARSAPM
                     AVNATYNRRLTITTHPASTLTNFPVRVSITDAALKTIANGGHVQNSNGYDIVFAADNA
                     YVTPYAWQMESYDGTAGTVDAWVQIPSVSSSVDTVFYMLYGDATIATFQSTSVAVWDS
                     HYKGVYHLSVGLNANDSTAGANNGTNNGATAVAAKIAGGAAFNGTSQYIDLGNNANLK
                     IALPATISMWINHNASYNPATNWDWFSNDVVWGGSGNRHGIDIGDDNTGKVVASFSDG
                     TSNYSLKIGTTTLSKGVDYFVTCVIRAHNDITIYLNGVDDGGSYAGAGGSSVGYGTGS
                     GAIGVSRGASAGGTNQGFYFGCIDELRVSDIERSAAWIAAEYTNQNSPGTFYTLAAEN
                     GQTFLHLRPRTSLRLALRAA"
     gene            2162..2479
                     /locus_tag="VGM72_07575"
     CDS             2162..2479
                     /locus_tag="VGM72_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265160.1"
                     /translation="MQKFDTTTPEGRLLLSVDFSRLLPPGVTIASAAVTIAVHTLSVA
                     DDSDATDRLDGSAIVSDDQTTISQWFQFGLDDVDYVLTFVASFSDGEIVPLAVEMPVR
                     LYS"
     gene            2547..2717
                     /locus_tag="VGM72_07580"
     CDS             2547..2717
                     /locus_tag="VGM72_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265161.1"
                     /translation="MGNFLNLLAGKKTYVIAAIAAATAAAQALGYAVPDWLYPIEGAL
                     GLGALRVAVNKS"
     gene            2720..2953
                     /locus_tag="VGM72_07585"
     CDS             2720..2953
                     /locus_tag="VGM72_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265162.1"
                     /translation="MTWLGILKALVLGVGVFADWLRDRQFMEAGRAETIASHLESALD
                     EIAKAQKARDAVYTDVMRDPGRLRDDDGFKRPD"
     gene            3485..3916
                     /locus_tag="VGM72_07590"
     CDS             3485..3916
                     /locus_tag="VGM72_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265163.1"
                     /translation="MNEILNGIRRERPGSLADVQGPNMGPQGEPHYRLKWLTPDGRVL
                     WLDTDARTGRVLGVEGDDRRPARPARGGLSGQRRNYYPDGPPVGPPNGYQRVPGGLGR
                     GLPPGYNGGPPPGYDRRSPSSYDNGPRPGPRAGSGRFRNGR"
     gene            3919..4599
                     /locus_tag="VGM72_07595"
     CDS             3919..4599
                     /locus_tag="VGM72_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4265164.1"
                     /translation="MRILVVEDDSDLQRLLKKALTDAGYVVDVASDGEEGHFLGDTEP
                     YDAVVLDLGLPKIDGVTVLERWRKAGRKMPVLILTARDRWSDKVAGFDAGADDYVAKP
                     FYTEELLARLRALLRRAAGIASPDIEVGPLRIDTRSSRVTVDGNPIKLTSLEYRLLSY
                     LAHHKGQVLSRTDLVEHLYDQDFDRDSNTIEVFIGRLRKKLGVDVIHTMRGLGYRLDE
                     PSAAGDET"
     gene            4677..5987
                     /locus_tag="VGM72_07600"
     CDS             4677..5987
                     /locus_tag="VGM72_07600"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY4265165.1"
                     /translation="MAGGFVLSGIFRGAVQENLDDRLFFDLDGLVAAAEVDAPEHVSL
                     QGRFADPRFERVFSGWYWQIEPDMPQTPDTQPQTSRSLWDQTLIPRNFTQGENNSLLG
                     YAAGPDNQSLRVVFRRIALPRTAEGREDTQVFRFMVAGDEKEMEADVARFNQTLFWAF
                     ALFSLGLLAAIAIQVRVGLMPLHRVSEALARIREGRARRLEGTFPAEIAPLVGELNSL
                     IAHGAEVVGRARAHVANLAHFLKTPLTVLANEASASPSPLADNVTRQVMSMRRQVDHY
                     LARARAAGALDVLGSRTEVAPVLNDLARVLKRMHPDKDLAIDLHVAPGIAFRGEREDF
                     EEMAGNLIDNACKWARSRIAVEVGPLEGGRLYLQVEDDGNGLAPDERSRAMERGERLD
                     ESVPGTGLGLAIVRDIAKLYGGDFTLGEATLGGLQARLELPRAA"
     gene            6106..7506
                     /locus_tag="VGM72_07605"
     CDS             6106..7506
                     /locus_tag="VGM72_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265166.1"
                     /translation="MLSTDKQLLLSGFLGKLPEHVAARLAKAVEVDRLIGGTSLPHDM
                     ILRALRPQLRQGPDRTLRMQTPQRFFCRPFEDILVGSERSIKQKGRITRDSIEAVWNW
                     LASDLMAVRHRIIVEALRDAILHARESEIEERCVELWSEAAAVLKPELATEKKKAFAA
                     KKLGGMALAEDAAEMALLLEAAPKIAELQGRLPKPIVNLSEDDLVFLRDCFEHWSDSD
                     PDLAPYVPLIVMGRLERRWEALRLVGALTRKSDDTLISNTDLGVVGELLFGDLDVYVK
                     KIQAIRPMDFDDAETLLGNLAGFAELSSGIVKEFGIRRDGKWGHRLTKDRGAVAQVME
                     DLLERAPKEILGALPAAKMGGFGKSPKPLDFSRAPDPERAAKAMRYAQLMRHAKPFAV
                     AAAFSAKLSETIDETTNVLRTHTEDILRELRAAAPETRGIVDLHWAQTLELCALVLGE
                     EETGLLRRRARVPAPA"
     gene            7614..7958
                     /locus_tag="VGM72_07610"
     CDS             7614..7958
                     /locus_tag="VGM72_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265167.1"
                     /translation="MAPKANPLKLNPLQLRTLTLLQALAVLPEAIEPGPGEGEVTVNR
                     FPQAHADHFHLGDAVVNGHDATGLFNQAVWNALSRKGLARADWPNAITLTPDGLSYET
                     GLAEEILRHSGH"
     gene            complement(7955..8935)
                     /locus_tag="VGM72_07615"
     CDS             complement(7955..8935)
                     /locus_tag="VGM72_07615"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEY4265168.1"
                     /translation="MRVRELLKAFLSAWATAAILTAGGAALAATKSEPGKITDATHGQ
                     GSPSPALWKIVNGKSTVYFLGSIHLLPEGFSWHTPAIDKAIKAADAFVFEADLDFGTA
                     EFHYYMDNYGYLPRGQTLHQMLSPAAQKQYFTLIRDMQIDLNKLDYLQPGLAVLLLDR
                     AFIGTHASRPLEPGVDAQLVYYAKSHAKELHYLESLQSQFDVLAKIGGGAQAAVLEKK
                     LLSREKNTGEFGTMVAAWVKGDLPKLISMDDMDPKERILTLDNRNRAWQPKIEAMLED
                     GRTYLVTVGTAHLAGPNSVISLLCAKHWKLERIQTGSTPPPPACADDRKS"
     gene            complement(9078..>10254)
                     /locus_tag="VGM72_07620"
     CDS             complement(9078..>10254)
                     /locus_tag="VGM72_07620"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY4265169.1"
                     /translation="KAQNFEDFTAVLASGGIPSLDFVYADKTGRIASLYNAAFPQRAD
                     GYDWTRAVPGDIRADMWNSYLAPSIGPRTIAPASGFVVAANAAPFRTTADPFNPKPES
                     FSPSLGIETLLNNRTRRALALFSADRSITAGEFRKYKFDDCYTPDSDFALLVKEIGGR
                     NYAGDPLLEEAGEILRRYNLCTDKSNRGTALAMMTAIPLLQASALRIAQPDPTATLRG
                     AANRLLAQFARLDPTWSQVSRLRRGDLDLAVSGGPDTLRSIDVQPRLDRAGTSTADAG
                     DSFTFISTWTKDGHWEVESVVPFGSSNVAGTPHYADQAALFADEELKTLPLSNSALTA
                     EASGIERPGKPPPPKGNIVVPPIPATSVPASNIIRPANAAERKPAAAPPVTARAPSQ"
BASE COUNT         1950 a         3034 c         3159 g         2111 t
ORIGIN      
        1 caaaccatgt tgcctgttct acacgacctc attgtcgggc ggcgttgaga cgtggtcttc
       61 acggatcgat atcgcagact acggtctggg atcggacggc ctcgggacgt ggttctattc
      121 cagcctcatg tatgtggcga ataacacgga gcttcacttc gtcgcgacga aggcaaagtc
      181 cgacaattcc atccgccagg acgttcatta tttcatctac gacacgacgg acggcagctt
      241 aaagaacttc gacaagagct tttctacggc ggcggggagc ctgccggttc tgatcgcaga
      301 cctggatgcg cactatcgaa tcttcgcgca gccgaccgcc ggcggttcaa cagggattcc
      361 caaattcctg tttgacgccg cgggccgtgc gaacgtcctg accatagacg gcacgcctgg
      421 cagcggcgat ttcgatgtat ggcacttgat ccttcagagc ggatcgtgga ccgatacgat
      481 gatcgggaac acgaatagcg aagtaaatga ctatgctcta attccggctg tggctggggg
      541 cgtgacggct tattttccac aagcggcgac agcggggttc gccgttgatg gcgattatta
      601 cacgacgacc cgagacgcaa gcaccggaac gtggagccct gtcgaattat tcctcgctag
      661 tgatgggata aacgcgctgg cagttggatt gccggtgttg aacgggattg ccgcgctgcg
      721 atttgttttt tcagaaatcc accaaagctc cgcagatagc ggcgccgggg ggcttcgcaa
      781 atatgcctac ggtgattttg gctatgttac ggcaaggagc gcgcccatgg ctgtgaatgc
      841 tacgtacaat cgccgcctca cgataacgac gcatcccgcc tcaacgctga cgaatttccc
      901 tgttcgcgtt agtatcacgg acgccgcgct caagactatc gccaatggcg gccacgttca
      961 gaacagcaat gggtatgaca tcgtctttgc cgccgacaat gcctatgtga cgccctatgc
     1021 ctggcagatg gagtcctatg atggaacggc cggaacggtc gatgcctggg tgcaaatccc
     1081 ttccgtgtcg tcgtcggtcg ataccgtatt ttacatgctg tatggtgacg cgacgatcgc
     1141 gacattccaa agtacgtcgg ttgccgtttg ggactcccat tacaagggtg tctaccatct
     1201 cagcgtcggc ctcaacgcga acgacagcac agccggcgcc aataatggca ccaataacgg
     1261 cgccacggcg gtcgcggcca agatcgccgg cggggccgcg ttcaatggca ccagccaata
     1321 tatcgatctc ggaaataatg cgaatctgaa gattgctttg cctgccacca tctcgatgtg
     1381 gatcaatcac aacgcctcct acaatcctgc aacgaactgg gactggttca gcaacgatgt
     1441 tgtgtggggt ggatcgggca atcgtcatgg catcgacata ggcgatgaca ataccggaaa
     1501 agtggtggcc tcattctccg acgggacttc gaattatagc ctgaagatcg gcacgaccac
     1561 gctctcgaaa ggcgtagact atttcgtcac ctgcgttatt cgcgcgcaca atgacatcac
     1621 catctatctg aatggcgtcg atgatggcgg aagttacgcg ggggcgggcg gcagttctgt
     1681 cggatacgga accggaagcg gcgcgatcgg cgtatcgcgc ggcgcgagtg ccggcggcac
     1741 aaatcaaggt ttctatttcg gttgcatcga tgagcttcgt gtctccgata tcgaacgctc
     1801 cgcggcatgg atcgcggcgg aatacaccaa tcagaattcc cctggcacgt tctacacgct
     1861 agcggctgag aatgggcaaa cgtttctgca tttgaggccg cgtacgagtc tgcggcttgc
     1921 cttaagggcg gcatgaacga aaacggtgct gcgcttgttc gtttcattgc ccacgtccaa
     1981 caggtgaatc atgttgctac ttctgtttgg tgtgccgagc accggcatct ctgtttttat
     2041 cgcggaaacc ggtgataacg tcgctcttcg cctcgcggcg gcggcgacaa cccctattga
     2101 tccccacttc tgcgtggtgg cgcctccgcg cctccgtacc ctcattccag cgagtaccaa
     2161 gatgcagaaa ttcgacacca ccacgccaga gggacggctc cttctgtcgg tggatttttc
     2221 gcgcctgttg ccgcccggcg tcaccatcgc gtcggctgcg gtgaccatcg ctgtccatac
     2281 gctttctgtg gccgatgatt ccgacgccac ggaccgcctc gatggttccg ccattgtgag
     2341 cgacgaccaa acaacaatct cgcaatggtt ccagtttggt ctggacgatg tcgattatgt
     2401 cctgacgttc gtcgcgagtt tctccgatgg agagatcgtg ccattggcgg tggagatgcc
     2461 tgttcgcctc tattcctgaa atttcgttct tgatatccac caaacccgcg tcgaaagccg
     2521 cgggtttttt ttaatgggag ctcatcatgg gaaacttttt gaatcttctg gccggcaaaa
     2581 aaacctatgt gattgcggcg atcgccgcgg ccaccgcggc ggcgcaggcg ctcggctatg
     2641 cggtgcccga ctggctctac ccgattgaag gcgcgctcgg acttggcgcg ctgcgcgtgg
     2701 ctgtgaataa gagttgaacg tgacatggct gggcattcta aaagcgctcg tccttggcgt
     2761 tggagtcttt gccgattggc tgcgcgaccg gcagtttatg gaagccggac gcgccgagac
     2821 catcgcatcg catctggaat cggcgctgga tgagatcgcc aaagcgcaaa aggcgcgcga
     2881 tgctgtctat actgatgtta tgcgcgatcc cgggcggctg cgcgacgacg atggtttcaa
     2941 gcgtcccgat tgagtccgat gcgtcattct gcgtggtggc gaagccgatt gcgtggtcgg
     3001 cccatgacag cgatgaaacc attcttcagg ttaaggagca caatgccgcc ggttcggcgc
     3061 tctgcggatg ggctgggcta gccggtgcgg cgggcggccc ggtgccccta atgccttcaa
     3121 aataaagaag accgaatggg caataattaa agagacccgc gggatcgctt ggaacccgca
     3181 ggtctctagc ttaagaccgg cctcgcgagg aggggcgtta cacgaaagac ggtctcaagt
     3241 tcttgatttg ccaatctcca ttgggtcggc cttctgtctg tgcctataat cgcgcaggca
     3301 ttctgaatgg aggctggcgg gcgcgttcat cgcgagttca tgcgccgcgg cataaaagtt
     3361 cccccaaatg cggtacgttg cgcccatatt ggttgctctc cttttgggtg cagctttgat
     3421 ttctccctca acggcacagc cctttcgcgc ccgtcagggg gtgcagtccg gcgaagtgcg
     3481 ttccttgaac gagatcctca atgggatcag gcgcgaacgc cccggcagcc ttgccgacgt
     3541 gcagggtccc aacatgggcc cgcagggcga gccgcattac cgcctgaaat ggttgacccc
     3601 cgacggacgc gtgctgtggc tcgataccga cgcacgcact ggcagggtcc tcggcgtcga
     3661 aggcgacgac cgccgcccgg ccaggcctgc gcgcggcggt ctgtccggcc agcgccggaa
     3721 ttattatccg gacggtcccc cggtcgggcc gccgaacggc tatcagcgcg tgccgggcgg
     3781 cttaggacgt ggtctgcctc cgggttataa tggcggcccg ccccctgggt acgataggcg
     3841 ttcgccttcc agctatgata atggcccccg acctggaccc agagccggat ccgggcgttt
     3901 ccgcaacggc cgctgaccat gcgcatcctt gtagtcgaag acgattcgga cttgcagcgc
     3961 cttctgaaga aggcgctgac cgatgctggt tatgtggtcg atgtcgcctc cgacggggaa
     4021 gagggacatt tcctcggcga cacggagccc tatgacgccg tggtgctcga tttgggcctg
     4081 cccaagatcg acggcgtaac cgtgctcgag cgctggcgca aagccggacg caagatgccc
     4141 gtgctcattc tcaccgcgcg cgaccgctgg agcgacaagg tggccggttt cgacgccgga
     4201 gccgacgatt atgtcgccaa acccttctac accgaggaat tgctggcgcg gctgcgcgcg
     4261 cttctcaggc gtgccgctgg gatagcgtcg cccgacatcg aagtcggccc gctccgcatc
     4321 gatacccgca gttcgcgggt gacggtcgac ggcaatccca taaagctgac ctccctcgaa
     4381 taccgcctgc tttcctatct cgcccatcat aagggccagg tgctctcccg caccgacctg
     4441 gtcgagcatc tttacgatca ggatttcgac cgcgattcca acaccatcga agttttcatc
     4501 gggcgcttgc gcaagaagct cggtgtcgat gtcattcaca ccatgcgcgg gcttggctac
     4561 cggctcgatg aaccgagtgc cgctggggac gaaacctgat cctagccttt tcccggccca
     4621 attcgctcac cttccggctg atcgtcggag cggcggtctg gagcgtcctt tgcctggtgg
     4681 ccggcggatt tgtgctgtcg gggatttttc gcggggcggt tcaggagaat ctggacgacc
     4741 ggctcttctt cgatctcgac ggcctagtcg ccgcagccga agtcgacgca cccgaacatg
     4801 tgtcgctgca ggggcgtttt gccgacccgc gcttcgagcg ggtgttctca ggctggtact
     4861 ggcagatcga gcccgatatg cctcaaactc ccgatacaca gccgcaaacc tcgcgttcgc
     4921 tctgggatca gacgctgatt ccgaggaatt tcacgcaagg agagaacaat tcgcttctgg
     4981 gttacgctgc gggtcccgac aatcagtcct tgcgcgtcgt cttccggcgc attgccctgc
     5041 cgcgcaccgc cgaaggacgt gaggacacgc aggtcttccg cttcatggtc gcgggcgacg
     5101 agaaggagat ggaagccgac gtcgcgcgct tcaaccagac attgttctgg gcctttgccc
     5161 tgttcagcct tggccttctc gccgccattg ccattcaggt gcgggtcggc ctgatgccgc
     5221 tgcaccgtgt cagcgaagcg ctcgcgcgca tccgcgaggg ccgggcgcgg cgtctggagg
     5281 gcacattccc ggccgaaatc gcacccctgg tgggcgaact caacagccta atcgcgcatg
     5341 gcgccgaagt ggtgggccgc gcccgcgccc acgtcgccaa tcttgcacat ttcctcaaga
     5401 cgcctttgac cgtgctggcg aacgaagcgt ccgcctcgcc ttcgccgctt gccgacaatg
     5461 tgacgcgcca ggtcatgtcc atgcggcggc aggtcgatca ctatcttgcg cgcgcgcgcg
     5521 cggccggcgc gctggatgtc ctgggcagcc gtaccgaagt tgcacccgtg ctgaacgatc
     5581 tcgcgcgggt gttgaagcgc atgcatcccg acaaggatct ggccatagat ctgcacgttg
     5641 cgccgggcat cgcgtttcgc ggggaacgcg aggatttcga agagatggcc ggcaacctga
     5701 tcgacaatgc ctgcaaatgg gcgcggtcgc ggatcgcggt agaggtgggg ccgctcgaag
     5761 gcggccgtct ctatctgcag gtcgaagacg acggcaacgg cctggccccg gacgagcgat
     5821 cgcgcgcgat ggagcgtggc gagcggctgg atgaatcggt gcccggaacc ggtcttggac
     5881 ttgccatcgt gcgcgacatc gccaagcttt atggcggcga tttcacgctg ggtgaggcca
     5941 cgctcggcgg gcttcaagcc cggcttgaac tgccgcgcgc ggcttagcgc cgtcacaatc
     6001 caccgttttc cggaaaattg gacaatgacc cattaacctt gcgcgtctag tgtgtccggt
     6061 cgagacctcg aagagagccc ttttgggggg cgatgcagct tgggcatgct cagcaccgac
     6121 aaacagcttc tgctcagcgg ctttctcgga aagctgcctg agcatgtcgc cgcgcgcctg
     6181 gccaaggcgg ttgaggtcga ccggctgatc ggcggcacga gtttgccgca cgacatgatc
     6241 ctgcgcgcgc tccggccgca attgcgccag ggcccggacc gtacgctgcg catgcagaca
     6301 ccgcagcgtt ttttctgccg gccgttcgaa gacattctgg tcgggtccga acgcagtatc
     6361 aaacagaaag gccggatcac gcgcgattcc atcgaggcgg tatggaattg gctcgcgagc
     6421 gacctcatgg ctgtgcgcca tcgaatcatc gtggaggctt tgcgcgacgc gattcttcat
     6481 gcccgagaga gtgagatcga ggagagatgc gtcgagttgt ggagcgaagc cgcagcggtt
     6541 ctgaaacccg aattggcgac tgagaagaag aaggccttcg cggcgaagaa actcggcggc
     6601 atggcgcttg ccgaggacgc cgcggaaatg gcgcttctgt tggaggcggc tccgaaaatc
     6661 gccgagcttc aggggcggct tcccaagccg atcgtcaacc tgtccgaaga cgaccttgtc
     6721 ttcctgcgcg attgtttcga gcactggagc gattccgatc ccgatcttgc tccctatgtg
     6781 ccgctcatcg tcatgggacg cctcgagcgt cgctgggagg cgttgcgcct ggtcggcgcg
     6841 ctgacgcgca aatccgacga cacgctgatc agcaacacag acctcggcgt ggtgggcgaa
     6901 cttttgttcg gcgacctcga cgtctatgtg aagaagattc aagccatacg gccgatggat
     6961 ttcgacgacg cggaaacatt gctcggcaat cttgcgggat tcgccgaact ttccagcggc
     7021 atcgtgaagg agttcggcat caggcgcgac ggcaaatggg gccaccgctt gaccaaagat
     7081 cgcggcgctg tggcacaggt catggaagac cttctggaac gcgcgcccaa agaaattctg
     7141 ggcgccttgc ccgccgccaa gatgggtggt ttcggcaaga gcccgaaacc actggatttt
     7201 tcgcgggcgc ccgatcccga gcgtgccgcc aaagccatgc gatacgctca gttgatgcgg
     7261 catgccaagc cttttgcggt ggccgcggca ttcagcgcca agctcagcga aaccatcgat
     7321 gaaaccacca atgtcctgcg cacccatacc gaggacatct tgcgcgagtt gcgcgccgcc
     7381 gcgcctgaaa cgcgcggcat cgtcgatttg cattgggccc aaactctgga gctttgcgcg
     7441 ctggtgctag gcgaggagga gaccggtctt ctgcgccgcc gggctcgcgt gccggcaccg
     7501 gcctgatgcc cttcttgcgg tacgaaacgt caaatttgcg catcgtttaa tcggaaaatg
     7561 ccggattaag tctcatctcg acggcgggtg ttcttcgtgc cacaaggcgc gtcatggcgc
     7621 cgaaggcaaa tcctctgaag ctcaacccgt tgcaattgcg cacgctgacg cttttgcagg
     7681 cgctcgcggt gttgcccgaa gccatcgagc ccgggcccgg tgagggcgag gtgactgtca
     7741 atcgtttccc gcaagcccat gccgatcatt tccacctcgg cgatgcggtg gtgaacggcc
     7801 atgatgcgac cggcctcttc aatcaggccg tttggaatgc gctctcacgc aaaggactcg
     7861 cgcgagccga ttggcccaat gccatcacgc tgacgcccga tgggctgtcc tacgagacgg
     7921 gattggcgga agagatcctg cgccactcgg ggcattagct tttacggtca tcggcgcagg
     7981 ccggcggcgg tggcgtggag ccggtctgga tgcgttccag tttccagtgc ttcgcgcaaa
     8041 gaaggctgat tacgctgttc ggccccgcca gatgcgccgt ccccacggtg accagatatg
     8101 tcctgccatc ctccagcata gcctcgatct tgggctgcca cgcgcgattg cggttgtcga
     8161 gcgtgaggat tctctccttc gggtccatgt cgtccatgga tatcaatttc ggcaaatcgc
     8221 ccttgaccca tgcggcgacc atcgtcccga actctccggt gttcttctcg cggctgagaa
     8281 gttttttctc aagcaccgcg gcctgcgcgc caccgccgat cttggcgagg acatcgaact
     8341 gggattgcag gctttcgaga tagtgcaatt ccttggcatg ggactttgcg taatagacga
     8401 gttgtgcgtc gacgccaggt tccagcggcc gcgaagcatg tgtcccgatg aaagcccgat
     8461 cgagcaacag caccgcaaga ccgggctgga gataatccaa tttgttcaga tcgatctgca
     8521 tgtcccgtat gagcgtgaaa tattgcttct gcgcggccgg cgacaacatc tgatgcaggg
     8581 tctggccgcg cggcaaatac ccgtaattgt ccatgtaata gtggaattcc gcggttccga
     8641 aatcgagatc ggcctcgaac acgaacgcgt cggccgcctt gatggcctta tcgatggccg
     8701 gcgtatgcca agagaaaccc tcgggaagga gatggatcga gcccaggaaa tagactgtgc
     8761 ttttgccgtt tacgatcttc cagagtgcgg gagatggcga gccttgccca tgcgtggcgt
     8821 ccgtaatttt gccgggctcg gatttggtcg cggcgagtgc ggcgccgccc gcggtcagga
     8881 tcgcggcggt cgcccaggcg gacagaaaag ctttcaaaag ctcgcgaacg cgcattccac
     8941 aacgctccaa ggatgctcgt tccgctatat agaaagcgaa ttaaaaccga gcgaacgaaa
     9001 gcgctctctc gcgtctttct tttgcgttcg gtaaccaaat cattgtcagg atttttcgcc
     9061 agctatcgcg caaatagtca ctgggaaggc gcgcgcgcgg ttaccggcgg ggccgctgca
     9121 ggtttacgtt cggcggcgtt ggcggggcgg ataatattgg acgccggcac cgatgtggcc
     9181 gggatgggcg gtacgacgat attgcctttc ggcggcggtg gttttcccgg acgttcgatg
     9241 ccgctggcct ccgccgtgag cgccgagttc gataacggca gagtcttcag ttcctcgtcg
     9301 gcgaaaagcg cggcctgatc ggcataatgc ggtgtgcctg ccacattcga gctgccgaag
     9361 ggcaccacgc tttcgacctc ccaatggcca tccttcgtcc aggtggagat aaatgtgaag
     9421 gaatcgcccg cgtccgccgt ggacgtcccc gcccggtcca gccgaggctg aacgtctatg
     9481 ctgcgcaggg tgtcgggccc gccgctgacc gccaaatcga ggtctccgcg tctaaggcgg
     9541 ctcacttgcg accaggtcgg atccaaccgc gcgaactgcg ccaacagccg gttggcggct
     9601 cctcgcagtg tggcggttgg atcgggctgc gcgatccgga gcgcggaggc ctgcaaaagc
     9661 ggaatcgcgg tcatcatcgc caacgcggtg ccgcggtttg atttatccgt acaaagatta
     9721 taacggcgaa ggatttcgcc ggcttcttcg agaagcgggt cgcccgcata attgcgccca
     9781 cctatttcct tgacgagcag ggcgaaatcc gaatcgggcg tatagcaatc atcgaattta
     9841 tacttgcgga attcgcccgc ggtgatcgaa cggtcggcgg agaagagcgc cagcgcccgg
     9901 cgcgtccggt tgttcagcag ggtttcgatt ccaaggcttg gcgaaaaact ctccggcttg
     9961 ggattgaagg gatcggccgt tgtacgaaag ggcgcggcat tcgcagccac gacgaatccg
    10021 cttgccggcg cgatggttcg cggaccgatg gatggagcaa gataactgtt ccacatatca
    10081 gcgcggatat cgcccggaac ggcgcgggtc cagtcatagc cgtcggcgcg ctgtggaaac
    10141 gctgcattat agagagaggc gatgcggcca gttttgtcgg catagacgaa atcgagactc
    10201 ggaattcctc ccgacgccag gacggcggtg aaatcttcga aattctgcgc cttg
//
LOCUS       DASYEG010000168        23077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3966651, whole genome shotgun sequence.
ACCESSION   DASYEG010000168 DASYEG010000000
VERSION     DASYEG010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 23077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23077
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3966651"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="VGM72_07625"
     CDS             <1..1209
                     /locus_tag="VGM72_07625"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY4265170.1"
                     /translation="DALKAGKVELAYQFASTPELGPDSGAAFMDAEFLAGWIALRNLH
                     KPELARHHFDRLAKGVTYPISVARAHYWLGRTADAMNNPAGAAEEYRVAAYYSATFYG
                     QLALTNISERPMLHMNNFVAADPLPSERAAFEADDRVQALRLLTQTGDRGMARLFALA
                     IAASTTDAKQLQMLAEMVASTGDQALAVKVAKNASYSDIYLQPYLHPLIPLPKFSGDA
                     PEAALVLGLTRQESEFDSGAVSVAGARGLMQLMPASAKRAASTSRLTYRPDDLNKPDY
                     NMQLGMTTLAEYLDRWDGSYILAIASYNAGPSNVRNWVEAYGDPRQPGTDPIDWIESI
                     PYPETRNYVQRVLENLQVYRNRLSNSDQRLVILSDLYRPNPVAAGAIQQANSAVPGAD
                     APIPAPPPPQ"
     gene            1206..2090
                     /locus_tag="VGM72_07630"
     CDS             1206..2090
                     /locus_tag="VGM72_07630"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4265171.1"
                     /translation="MKRFEEHFTLSHDGLRLFYRDYAGPPAQMPARPPVLCIPGLSRN
                     SRDFDFIAGHIAQTRRVLVTDLRGRGQSDYDPDPRNYSVPVETGDVMQILGTAGIAQV
                     VVLGTSRGGVIAMTLAALAPAVLAGAILNDMGAQLEAAGLERIYAVMRSPPSFSNWED
                     AASALRRTNMALFPNVSDERWRVFAHALYREENGRVVGDYDPKFPGAILEGRGTNPRN
                     PAGAADLWKWFGALAAIPALVLRGENSELLSAETIAAMKTAKPDLVSITVKDRAHVPF
                     LDEPEAVFAIDAFLERIA"
     gene            complement(2104..2373)
                     /locus_tag="VGM72_07635"
     CDS             complement(2104..2373)
                     /locus_tag="VGM72_07635"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265172.1"
                     /translation="MAYVDMDALTEFESAARNGRADALYNLGLAYSTGQGVRVDYIAA
                     HKWFNLAAMKGVNEARTWRAQLSREMDSGQVAEAQRQAREWLRHP"
     gene            complement(2575..3363)
                     /locus_tag="VGM72_07640"
     CDS             complement(2575..3363)
                     /locus_tag="VGM72_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005095793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEY4265173.1"
                     /translation="MPQARSKASAARKIRAIPKSAAEPSRKLRQRPIELYFWPTYNGF
                     KISIMLEECRLPYELKPVNINRGEQFAPHFLSIAPNNRMPAIIDPDGPGGRSISIFES
                     GAILQYLGRKTGKFYPRDERRRVDVDQWLFWQVSGFGPMAGQAHHFRGYAADQIPYAI
                     DRYTNEMNRLYGVMNRRLADRDFLAGAYSIADMACWGVVKSWERQGQNIEEFPNVHAW
                     WERMNARPGVERGGNVGAYLRDPNYQLGKDAEAVKVLFGQRART"
     gene            complement(3532..3789)
                     /locus_tag="VGM72_07645"
     CDS             complement(3532..3789)
                     /locus_tag="VGM72_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265174.1"
                     /translation="MPMTAKKKPARKKAVKAKKTTVRKAAAKKPARKVAKKPGRKAAP
                     KKAAAKRKVVKKATKKPARKAAKKPAKRRAKKAAAPAAPMM"
     gene            complement(4033..5517)
                     /gene="gatB"
                     /locus_tag="VGM72_07650"
     CDS             complement(4033..5517)
                     /gene="gatB"
                     /locus_tag="VGM72_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005247018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEY4265175.1"
                     /translation="MSEAPPRSARPIEGATGKWEIVIGMEVHAQISSKAKLFSGAATQ
                     FGAAPNSQVSLVDAAMPGMLPVINRYCVEQAVRTGLGLKAKINLHSVFDRKNYFYPDL
                     PAGYQISQYKQPVVGEGEIHLDLPNGETVAVGIERLHLEQDAGKSLHDQHPDFTYVDL
                     NRAGVALMEIVSRPDMRSSEEAAAFLRKMRAILRYLGTCDGNMEEGSMRADVNVSVRR
                     TSEPLGTRCEIKNVNSMRFVQQAVDYEARRQVDILESGGVIRQETRLFDSRNGETRPM
                     RSKEEAHDYRYFPDPDLLPLTFDQAWVDALARALPELPDEKKVRFTASGLSAYDASVL
                     VAEKEAADYFEAMVAEGADAKAAANWLNNEYFGRLNKAGLTIATGLISAEAASAIVQM
                     VAAKTISGKIAKDLADIVWREGGDPKLLVETRGLRQVTDETAIEAAVDAVIAANPERC
                     AEVKAKPKAAAWFVGQVMKATQGKANPQTVNTILRRRLGLPDEG"
     gene            complement(5517..7007)
                     /gene="gatA"
                     /locus_tag="VGM72_07655"
     CDS             complement(5517..7007)
                     /gene="gatA"
                     /locus_tag="VGM72_07655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEY4265176.1"
                     /translation="MARPSDPTQFTIAQAREALAAKDISASELVDAHVQAVAKARALN
                     AFVTETPEHALSAAESSDARIATGQARVLEGIPLAIKDLFCTVGVRTTAASRILDNFV
                     PTYESTVTQNLWNAGAVMLGKTNLDEFAMGSSNETSYFGPVTNPFRAHGRNEDLVPGG
                     SSGGSAAAVSAHLALGATGTDTGGSIRQPAAVTGIVGMKPTYGRCSRWGIVAFASSLD
                     QAGPMTRTVQDAAIMLGVMAGHDPRDSTSVDVPVPDYEPMLGAGVKGLRVGIPKEYRI
                     DNMPPEIDALWQRGIDWLKAQGAEIVEVSLPHTKYALPAYYIVAPAEASSNLARYDGV
                     RYGHRAPGVDDLIGLYERTRAEGFGREVKRRIMIGTYVLSAGYYDAYYLQAQKVRTLI
                     ARDFSQAFAACDVLLTPATPCAAFGIGAKTADPVSMYLNDVFTVTVNLAGLPAISVPA
                     GLSHDGLPLGLQIIGKAFDEATVFRTGRAIERAADFKAAPQSWWRM"
     gene            complement(7007..7294)
                     /gene="gatC"
                     /locus_tag="VGM72_07660"
     CDS             complement(7007..7294)
                     /gene="gatC"
                     /locus_tag="VGM72_07660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEY4265177.1"
                     /translation="MSVDRETVRRIARLARLAVDEDQTAAMETELNALLSWVEQLSEV
                     DVTGVDPMTSVVEQKLKMREDIVTDGGYAADLMKNAPQSEDNFFVVPKVVE"
     gene            7406..7882
                     /gene="ruvX"
                     /locus_tag="VGM72_07665"
     CDS             7406..7882
                     /gene="ruvX"
                     /locus_tag="VGM72_07665"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEY4265178.1"
                     /translation="MAVVDLDQFRSHLRAGQRLLGLDLGEKTVGLALSDTSLTVATPL
                     KTLARGKFRNDATSIAVLIVEHDIGGLIVGLPLNMDGTDGPSAQSARAFARNFAAVSD
                     CPICLVDERLSTAAVTRTLIDADASRRRRGQVVDKMAAAYILQGALDRLRNSHPRD"
     gene            complement(7872..8315)
                     /locus_tag="VGM72_07670"
     CDS             complement(7872..8315)
                     /locus_tag="VGM72_07670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEY4265179.1"
                     /translation="MPAAKLRPKPCQFCEIAAGTGKAFVVFADDFSVAFLDYRPLMPG
                     HVLLVPRGHYETLAETSDEVMAGLGVRVKWLSAAVMIGLGVEGSFVALNNVVSQSVPH
                     VHFHIVPRRKGDGLFSTGLIWKRRGYRDDEEREAIAAKIRAALNP"
     gene            8417..9802
                     /locus_tag="VGM72_07675"
     CDS             8417..9802
                     /locus_tag="VGM72_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265180.1"
                     /translation="MQTLRHVTSINDLSLPEIETVFSLAQSYLRNLRDPNLPHRIGRS
                     ISDAQGSVLASLFYEPSTRTRLSFESAMLRLGGKSISSADPATSSAAKGESLADTVRV
                     ASNYADVLVIRHPRDGAAKLAATYASVPVMNGGDGAHEHPTQTLCDLFTLLQEGKNLK
                     HLNVAISGDLKGSRTIHSFVYALARFGANIMMMPAKGMELPSHVDWRLRNELGCYAVP
                     RARYEKDNASVDALYMTADKPHQLSLISNVALKVRKNIDAMYVTRFQKERWTDKTQDY
                     PTIDQRFLKDKKFSKTSLLHPLPRVGELDISLDNDRRAAYFRQAAFGVPIRMALIALI
                     LGMGRDYALKQYPGGFKPDEHPVYDQPNDTGITCANTNCIVHDPQEGKYARNRFHLVG
                     ANRLRCFYCETDIVDFVVAHKKSKHYETDVSKLHAFASKDRDIVIYPQAAEAEAAGFS
                     LPKRRRKTAGG"
     gene            9804..10430
                     /gene="plsY"
                     /locus_tag="VGM72_07680"
     CDS             9804..10430
                     /gene="plsY"
                     /locus_tag="VGM72_07680"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEY4265181.1"
                     /translation="MIETLGLVPWETAWPYIFATFFASYFLGSIPFGLIFARIAGAGD
                     IRKIGSGNIGATNVLRTGKRWAAAATLFCDGAKGFLAVWYAGEKFGIQFFPVLAGFGV
                     FLGHLYPVWLRFKGGKGVATFIGIVFALASPVGLLVCVSWLGLAQIFRYSSVAALIST
                     ALTPFYFLFLGATLFALLTFILVALIFISHRTNIGRLIRGDEPKIGAR"
     gene            10427..11545
                     /gene="dprA"
                     /locus_tag="VGM72_07685"
     CDS             10427..11545
                     /gene="dprA"
                     /locus_tag="VGM72_07685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY4265182.1"
                     /translation="MMRPRPLRSDERRAWLRLARTETIGPATFAALMSRFSDAREALD
                     AAPRLARRGGAQQLRMPSDADAEAEIEALTNLGGRLLACVEPDYPSGLAALDPPPPLI
                     SVLGHSALLSRDMVAMVGARNASALRIKFAARLAADLGAAGLVVVSGLARGIDHAAHR
                     GALENGTVAVLAGGVDIVYPPEHQALYEEMKTRGALVSEMPLGMAPVARHFPRRNRLI
                     SGMARGVVVIEAAERSGSLITANYALEQGREVFAVPGSPLDPRAKGANRLIREGATLT
                     EGADDVLAVLRPILGHAFQEPSERAVSPPSVDEIAVEREADRVRILVEEKLGPSPVEV
                     DELIRQCGAPPAAVLTVLLELELAGRAQRQPGNRVSWR"
     gene            complement(11542..12021)
                     /locus_tag="VGM72_07690"
     CDS             complement(11542..12021)
                     /locus_tag="VGM72_07690"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEY4265183.1"
                     /translation="MKEQDVEYLRMALAEAKAAGERGEVPVGAVLVSAEDDLLALSGN
                     RILEIRDPTAHAEMLAIKEGARRIGNERLVGTTLYVTLEPCGMCAGAVSLARVGRLVY
                     AAPDPKGGAVEHGPRFFALDTCHHRPVIERGWGAEEAGTLLRDFFRARRVKAGESSR"
     gene            12045..12866
                     /locus_tag="VGM72_07695"
     CDS             12045..12866
                     /locus_tag="VGM72_07695"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY4265184.1"
                     /translation="MTKTDEVPELERGERIAKVIARAGVCSRRDAERLIAEGRVALNG
                     EKIASPALNVPADALITVDGKPIPTASPTKLWRYHKPAGLVTSHRDPQERATVFEKLP
                     RSMARVISVGRLDINSEGLLLLTNDGALARQLELPSQGWVRRYRVRLFGKVTQGDLDR
                     LAKGVTIDGVRYEPVTAELERSKTSNAWAMVSLKEGKNREVKRLMEFLGFKVSRLIRV
                     SFGPFHLGHLPEGAVDEIPGKVWREQLGLTGKGKAEREERPAGARTRGRGKTRAP"
     gene            complement(12868..13485)
                     /gene="pdxH"
                     /locus_tag="VGM72_07700"
     CDS             complement(12868..13485)
                     /gene="pdxH"
                     /locus_tag="VGM72_07700"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEY4265185.1"
                     /translation="MSATEAEGEVKPSAEPFEIFGEWFAEAKAQEPNDPDAMSLASVD
                     ATGLPDVRMVLLKQADHEGFVFYTNLESAKGEELAATPKAALCFHWKSLRRQIRVRGP
                     VSPVSDTEADAYFATRPKDSQIGAWASRQSRPLEGRFALEKEVAFFAAKFALSTVPRP
                     THWSGFRLRPVVIEFWRDRPFRLHERIRYRREAPRGPWTTEHLFP"
     gene            complement(13482..14672)
                     /locus_tag="VGM72_07705"
     CDS             complement(13482..14672)
                     /locus_tag="VGM72_07705"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4265186.1"
                     /translation="MISASVAFLETPRTSYGSRIFASFDVLYRKSLPFKVSPVASTPI
                     RYDERQKHRQEVNMVVSRFGRKVLPGLLLCLAALPAWAAGKLVSLDVALGDVSLNKVS
                     FLIAADAGIYAKNGLDVHQFITPGAAQVARNSGIVVPAENVKADIGDAPISIGGGSPM
                     IYRVANGGGVHRIVLATSESIIRDHIIASTDIKSVADLKGKRLGYSVPGAVTHVGAVG
                     FAKVMGWEPGKDITLVGGGNALNPLREGREDALLGSGMLFSRASDLKFKDLIDLTQYK
                     IPVAGSSILAEKNWLAANRDTAARFVKAALEADALMKTNRGAFDAALVKWFNIKDTPT
                     QQSMYREAVDIPRKPYPAVEGIKSTLAIYDSPQMRKYKAEDFYDSSFIAALDKSGAID
                     RLYK"
     gene            14614..15573
                     /locus_tag="VGM72_07710"
     CDS             14614..15573
                     /locus_tag="VGM72_07710"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEY4265187.1"
                     /translation="MRDPYEVLGVSKKATEAEIKKAFRALAKKFHPDTHANDLKAVKK
                     FQEISAANEIVGDKEKRAQYDRGEIDAAGQPKGFSQGHHPGGFEQGFGGFRGRPGGGA
                     GPQGFEYEFTGNGPEGFQADDIFADILGGLGGGGRRRSAQPRKGNDVQLQTTVSLQEA
                     ALGGTRRVVLADGRQLEVRIPPGVKEGQQIRLRGQGAPGERGGVAGDALITIAIAPHP
                     YFERDGRDLRLDLPITLKEAVSGAKVPVPTLTGPVTVTVPPHSNSGRVLRLKAKGLPG
                     TGGDPAGDLYARLVITLPEASDPELDDFVKDWKHDYDPRAKLK"
     gene            15646..16470
                     /gene="fabI"
                     /locus_tag="VGM72_07715"
     CDS             15646..16470
                     /gene="fabI"
                     /locus_tag="VGM72_07715"
                     /inference="COORDINATES: protein motif:HMM:NF006019.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEY4265188.1"
                     /translation="MAVPGIMTGKRGLIMGVANDRSIAWGIAQSLAAQGAELAFTYQG
                     DAFKKRVVPLVEPLSPAALIDCDVSQPASLDAAFAELSAKWDRLDFLVHAIAFSDRDQ
                     LKGRYVDTTAENFTMTMNISCYSFTAVANHAEKMMTNGGSLLTLTYYGAEKVMPHYNV
                     MGVAKAALEASVRYLAEDLGKKNIRVNALSAGPIRTLAASGVGDFRYILKWNELNAPL
                     RRTVTTAEVGDSALFLLSDLSRGITGEVMHVDSGYHIVGMKAEDAPDIAVVRSDGS"
     gene            16460..17089
                     /locus_tag="VGM72_07720"
     CDS             16460..17089
                     /locus_tag="VGM72_07720"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY4265189.1"
                     /translation="MALELQQGVTLHFARHGETIANVEHRFQGRNDTPLTDRGREQAH
                     TIAAILSDSLAGNPTPRFVASPLPRTRTMMEIVLDALHLPRVYDTDPRLMEIDLGAWS
                     GLTDAEACALDPEMWERRIHDKWNVRVPGGGENYAMVAERLTAWAKEVNRDTVAISHG
                     AATRILRGLLLGLGWKEMSDLDEPQDCVFRYRDGTLTRLENHAPPPGPV"
     gene            17123..18223
                     /gene="aroC"
                     /locus_tag="VGM72_07725"
     CDS             17123..18223
                     /gene="aroC"
                     /locus_tag="VGM72_07725"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY4265190.1"
                     /translation="MSYNSFGHLFRFTTFGESHGPAIGCVVDGCPPGIALTEADIQVY
                     LDRRKPGQSKFVSQRKEPDAVRILSGVFKDERMAEQVTTGTPIALLIENVDARSKDYG
                     DIRDKFRPGHADYTYWAKYGFRDYRGGGRQSARETASRVAAGAVARKILDAMYGAVTI
                     RAALIQMGTLTIDRGRWDWDEVLRNPFNAPDAGAVDPWTDYLERLRKSGSSCGAIVEV
                     VADGIPVGLGAPVYGKLDADLASAMMSINAVKGVEIGAGMGAAALTGEENADEMRRGP
                     DGNPIFLSNQAGGILGGISTGQQIVVRFSVKPTSSILTPRKTIDVAGNETEIMTKGRH
                     DPCVGIRAVPIGEAMMACVLADHALRHRAQMG"
     gene            complement(18220..18984)
                     /locus_tag="VGM72_07730"
     CDS             complement(18220..18984)
                     /locus_tag="VGM72_07730"
                     /inference="COORDINATES: protein motif:HMM:NF013788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HEY4265191.1"
                     /translation="MMLARVFLVVLAAALSLLAAPASAAGPFDNWAALVVAGDFRAHD
                     GAPSPVFDNARRDLVTALKQMGFAANHIQQYSARPELDPQTKPLRSDGPAIYAGFEKL
                     TETATGGCFVYFTSHGAPLGILVGDQVVAIRSVGDIIDNSCHDRPTVAIISACFSGMF
                     IPVLRADNRMILTASRPDRTSFGCGEDLKYTFFDQCVLETLPMVQNFSALADKVKDCV
                     AEREMKEGATPPSEPQVYIGKTIAPALAQTYTFTLN"
     gene            complement(19025..20242)
                     /locus_tag="VGM72_07735"
     CDS             complement(19025..20242)
                     /locus_tag="VGM72_07735"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265192.1"
                     /translation="MTETVEIVRLGHAGDGVITDNLFVPYTVPGDVVRIAREGARGRL
                     LEIVTPGASRAQPACRHFGRCGGCALQMMAREPYLAWKRDLVVTALKQRGFTDVPVED
                     IRAVPPGTRRRAMFKARANEHAVSLGFYEPESRRLVDIAECPVLSSELASLIAPLKLR
                     LAPLLRAGETAELHATAADNGIDLSLKVKRARDPDFLMMLSELAASLKLARLSWNGEL
                     VALAAAPVLRMGRFSVALPPESFLQPTKEGMRILQDLVCEGAKGARHVADLFSGCGTF
                     ALALADGRDVHAVDSAEAQIAALTAAAKAGKANLTAETRDLFRRPLLASELARFDCVV
                     LDPPRPGAVAQAEILAQSAVSEVLYISCNPASFSRDARILADGGYRLMRVVPLDQFLW
                     SPHVELFAAFARA"
     gene            complement(20476..21522)
                     /locus_tag="VGM72_07740"
     CDS             complement(20476..21522)
                     /locus_tag="VGM72_07740"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY4265193.1"
                     /translation="MRKDVLKALVLQGGGALGAYELGVARALYSDEGPIPDLIAGVSI
                     GAITAALLARPKNGDPLGTLEAFWKKVTLRADFLLPALRPYASLFGDPAFFRPRLDVF
                     AFPSWTSLYDTAPLRVTLAELIDLDALADTSARPRLLLTATDIEAGEITPFDSADMPL
                     TLDHILASGSLPPNFPMTGIGEKFYWDGGIFDNTPLGAVIERMNPQKECESARQIIVV
                     NLFPNAGDVPKNMAQVSQRILNLLFANKTRSDLKLLGRFNHVAALMDLINSDERWAEL
                     RKTDEFAGANKGYIVVPDIVESTHSAAAAGSSSGDFSPDAIAMLADAGERDARVKLGM
                     TPKTEAREARRAAG"
     gene            21666..22694
                     /locus_tag="VGM72_07745"
     CDS             21666..22694
                     /locus_tag="VGM72_07745"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4265194.1"
                     /translation="MNENDFPPSKHVTRRIALGAVAAAATAAAGPVLGVTAPSEGCRI
                     GPAPHPKGPLVFMNYDQIELDAAYDQSAYAPLGAQIQARRSTNSEEVRRRLGAPRRES
                     YGPTEIEKLDIFRTTRPNAPVFIFAHGGAWLGGDAKEYHFAAENMVTAGAHYIALDFL
                     AVGAANGDIRVMADSVKRAIAWVYKNAASFGGDPNHIYLCGQSSGAHLSGVALITDWQ
                     KDFGLPADIVKGGVLLSGMYEMKPVRLSARSKYVKFDDAMEDSMSTIRHIDRLHTPIT
                     LLYGTNETPEFQRQSRDFAAAVKAAGKPVTLIVAPNHNHYELQETMANPYGWAGRAAL
                     AMMGMGKA"
     gene            22733..>23077
                     /locus_tag="VGM72_07750"
     CDS             22733..>23077
                     /locus_tag="VGM72_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265195.1"
                     /translation="MAEDRREALLKEYGEVSSNFRLLTDIRFKLLAFLPAATAVATAL
                     KGDSLSAASAVLSLFGLAATIGLVTYNLRNDQLYDELVGRAAFIERSLGIPDGAFANR
                     PRPWLIFRFLHCI"
BASE COUNT         4490 a         7303 c         6895 g         4389 t
ORIGIN      
        1 gacgcgctga aggctggcaa ggtcgaactc gcctatcaat tcgcctctac cccggaactc
       61 ggcccggatt cgggcgccgc cttcatggac gccgaattcc tcgccggatg gatcgcactg
      121 cgcaatctgc acaaacccga actggcccgc catcatttcg accggctcgc aaagggcgtg
      181 acctacccga tcagcgtcgc gcgcgcgcat tattggctag gccgcaccgc cgatgcgatg
      241 aacaatcccg ctggagcagc cgaagaatat cgtgtcgcgg cctattattc ggccacattc
      301 tacgggcagc tcgcgcttac caacatctcc gagcggccaa tgctgcacat gaacaatttc
      361 gtggcggccg atcccttgcc gtccgagcgc gctgccttcg aggctgacga ccgggttcag
      421 gcgctgcggt tgctcacgca gaccggcgac cgcggcatgg cgcggctttt cgcgctcgcc
      481 atcgccgcca gcaccaccga cgcaaaacaa ctgcaaatgc ttgccgaaat ggtggcgtca
      541 accggcgatc aggcattggc ggtgaaagtc gccaagaatg cgagctacag tgacatctac
      601 cttcaaccct atctgcatcc cctcattccg ttgccgaaat tctccggcga cgcaccggaa
      661 gcagccctcg tgctcggcct gacgcgtcaa gaaagcgaat tcgattcggg tgccgtcagc
      721 gtcgcgggcg cgcgcggcct gatgcaattg atgcccgctt cggccaagcg cgcggcgagc
      781 acctcgcgcc ttacctaccg gccagacgat ttgaataagc cggattacaa catgcagctc
      841 gggatgacga cgcttgccga atatctggac cgctgggacg ggtcgtatat tctcgccatc
      901 gcgtcctaca atgccggccc ttccaacgtg cgcaattggg tcgaggccta tggcgacccg
      961 cggcagccag gcacggatcc catcgattgg atcgaatcca tcccctatcc ggaaacgcgc
     1021 aattatgtgc agcgcgtgtt ggagaatctc caggtctatc gcaaccggct cagcaattcc
     1081 gaccagagac tggtcatcct ttccgatctc taccggccaa atcccgtggc cgccggcgca
     1141 atccagcaag ctaattcggc agtgcccggc gccgacgcac caatccccgc gccgccacct
     1201 ccgcaatgaa acgcttcgag gagcacttca cgctatccca tgacgggctg cggctctttt
     1261 atcgcgatta cgccgggcca ccggcccaaa tgccggcgcg gcccccagtg ctttgtattc
     1321 ccgggctctc gcgcaattcc cgcgacttcg atttcatcgc cggccatatc gcgcaaaccc
     1381 gccgcgtgct ggtgacggac ctgcgcgggc gcgggcaatc cgattacgat cccgatccgc
     1441 gcaattattc ggttccggtg gaaaccggcg acgtgatgca aatcctcggc accgccggga
     1501 tcgcgcaggt cgtcgtgctg gggacttcgc gcggcggtgt gatcgcgatg acgctggccg
     1561 cactggcacc tgcggtcctc gcgggtgcca tactgaacga tatgggcgcg caactggaag
     1621 cggcgggcct ggagagaatc tacgctgtca tgcggtcacc gccctccttc agcaattggg
     1681 aggacgccgc atcggcgctt agacgcacaa acatggcttt atttcccaat gtgagcgacg
     1741 aacgctggcg tgtcttcgcc cacgcgctct atcgcgagga aaatgggcgt gtcgtcggag
     1801 actacgatcc caagtttccc ggagccatcc ttgaggggcg cggtacgaac ccgcgcaacc
     1861 cggccggggc agcggatttg tggaagtggt tcggggcttt ggctgcgatt ccggcgcttg
     1921 tgctgcgcgg cgagaattca gaacttttgt cggccgagac aattgcggcg atgaagactg
     1981 cgaaacccga tctggtttcg atcaccgtga aggatcgcgc tcacgtgcca tttctggatg
     2041 agccggaggc ggtcttcgcc atcgatgcgt tcctcgaacg catcgcctga gcttcaaaga
     2101 ccgtcaggga tgccgcagcc actcacgcgc ctggcgttgg gcttcggcga cctgacctga
     2161 gtccatctcg cgcgagagtt gcgcccgcca agtccgcgct tcgttcacgc ccttcatggc
     2221 ggcgagattg aaccatttgt gcgccgcgat gtaatcgacg cggacgccct ggcccgtcga
     2281 atacgccaat cccaaattgt acagtgcatc ggcgcgccca ttgcgtgccg cggattcgaa
     2341 ttccgtcagc gcgtccatat ccacatacgc catcggctaa tctccccttc gtgaccggca
     2401 tccgccggaa gtccatgacc gctcgcgcgg tcgcctacgc gcggctgggc aaacgcgccc
     2461 gcacgccgcg tgacgatcat ggaaattttc ttcctaaagg gacgttaacg ctgcgcaata
     2521 ttgaggttaa tgcgcattag gattcaggcg caccgttgga gcgtctatgc gccgtcaggt
     2581 gcgggcacgc tggccgaaga gaacctttac cgcctcggcg tccttgccga gctgataatt
     2641 gggatcgcgc aaataggccc cgacgttccc tccccgttcg accccgggac gcgcattcat
     2701 gcgttcccac caggcatgca cgttgggaaa ctcttcgatg ttctggcctt gtctttccca
     2761 gctcttcacc acgccccagc atgccatgtc ggcaatggaa taggcgcccg caagaaaatc
     2821 gcgatccgca agccgccggt tcatcacgcc ataaaggcgg ttcatctcat tggtatatcg
     2881 gtcgatcgcg taaggaatct gatccgccgc gtaaccgcgg aaatgatggg cctggcctgc
     2941 catgggcccg aagccggaga cctgccagaa cagccattga tccacatcta cccggcgccg
     3001 ctcgtccctc ggatagaact tgccggtctt gcgaccgaga tattgcagga tcgctcccga
     3061 ttcgaagatc gagatcgaac gtccacccgg accatcggga tcgatgattg cgggcatgcg
     3121 attgttcggc gcaatcgaca ggaagtgggg agcgaactgt tcgccacgat tgatgttgac
     3181 cggcttcagc tcatagggca gccggcattc ctcaagcatg atgctgatct tgaagccgtt
     3241 ataggtcggc cagaaataaa gttcgatcgg gcgctggcga agcttgcgcg aaggctcggc
     3301 ggccgatttg gggatcgcgc ggattttgcg ggcggcagat gctttggatc gggcttgcgg
     3361 catggctatc ctcaggcaca gcagggggca agtttgagac ttagcccagc caacctttgg
     3421 cggcaaacct gatgaggaca gaaggagact ggaagaagaa aatcggtggc gggcaggccg
     3481 cggccctccg aagaggaacc gcggcctttg ccttcaccac ccgaaagcgc cttacatcat
     3541 tggcgctgca ggggcggcgg cttttttcgc gcgccgtttg gcaggcttct tcgccgcctt
     3601 acgtgcaggc ttcttggtgg ccttcttcac aactttccgc ttcgcggcgg ccttcttcgg
     3661 cgcagccttg cggccgggct tcttcgcgac cttacgggcc ggcttcttcg ccgccgcctt
     3721 acggacggtg gtcttcttcg ctttcactgc ttttttacga gcaggctttt tctttgcagt
     3781 catcggcatt gaaacgactc cctttagagt tcggtcggcg caacacgcac cttccaaaaa
     3841 cgtccggaaa tatgcggagg ttgggttaat gaagcaatac caagcccttg cgcggcaaaa
     3901 cccctgtcaa gcgcaaaatc aaaaaactca atgaaatatt atgttttaat gcatcgtctt
     3961 ggtacggacg aaaattcccg tttcaacctt gacaatgccg ccggagtgca aattttgaca
     4021 gaagccgacg aatcacccct cgtcgggtaa cccgagtcgg cggcgcaaaa tcgtgttcac
     4081 agtctgcgga ttcgcttttc cctgggtcgc tttcatcact tggccgacga accaggcggc
     4141 ggctttcggc ttcgccttca cctccgcaca ccgttccgga tttgcggcga tgacggcatc
     4201 gaccgccgct tcgatggcgg tctcatcggt cacctgacga agaccgcgtg tctccacgag
     4261 cagtttcgga tcgccgccct cacgccagac gatgtccgcc agatcctttg cgatcttgcc
     4321 tgagatggtc ttcgccgcca ccatctgcac gatggcgctc gcggcctcag ccgatatcag
     4381 gccggtggcg atcgtgagac cggctttgtt cagacggccg aaatattcgt tgttcagcca
     4441 gtttgccgct gctttggcgt ccgccccttc cgcgaccatg gcctcgaaat aatcggccgc
     4501 ttccttctcg gcgaccaaca ccgaggcgtc ataggcagac aagcctgaag ccgtgaaccg
     4561 gactttcttc tcgtcgggga gttcgggcaa cgcgcgcgcc aatgcatcca cccaggcctg
     4621 atcgaacgtc agcggcaaaa gatcgggatc ggggaaatag cggtaatcat gcgcctcctc
     4681 cttggatcgc atgggacgcg tctctccatt gcgggaatcg aacaggcgtg tctcttgccg
     4741 gatgacgccg cccgattcca gaatgtccac ttgccggcgt gcctcatagt ccacggcctg
     4801 ctgcacgaaa cgcatggagt tcacgttctt gatttcgcag cgcgtgccca gcggttcgct
     4861 cgtccgccgg accgacacgt tcacgtcggc acgcatggag ccttcctcca tattgccgtc
     4921 gcaggtgccg agatagcgca ggatcgcgcg cattttgcgc agaaacgcgg ccgcttcctc
     4981 cgatgagcgc atatcgggcc ggctgacgat ctccatcagc gccacacccg cgcgattgag
     5041 atcgacataa gtgaaatcgg gatgctggtc gtgcaggctt ttgccggcgt cctgttccag
     5101 atgtagccgc tcgatcccca cggccaccgt ttcgccgttc ggaagatcca gatggatctc
     5161 gccctccccc accacgggct gcttgtattg ggagatctga taacccgccg gcagatcggg
     5221 atagaagtaa ttcttccggt cgaacaccga atgaagattg atcttcgcct tcagcccaag
     5281 accggtgcgg accgcttgtt cgacgcaata gcggttgatc acgggcagca tgcccggcat
     5341 ggcggcatct accaagctca cctgcgaatt cggggcggca ccgaactgcg tcgcggctcc
     5401 ggaaaaaagc ttcgccttgg aggatatctg cgcatgcacc tccatgccga tcacgatttc
     5461 ccatttgccg gtggcgcctt cgatcggcct ggcgcttcgg gggggtgctt cgctcatcac
     5521 atcctccacc aggattgcgg cgcggctttg aaatcggcgg cgcgttcgat ggcgcgcccc
     5581 gtgcgaaaaa cggttgcctc gtcgaatgcc ttgccgatga tctgaaggcc gagcggcaga
     5641 ccgtcatggg aaaggcccgc aggaaccgag attgcgggca acccggcaag gttcaccgtc
     5701 actgtgaaca cgtcgttgag gtacatcgag acggggtctg cggtcttggc gccgatgccg
     5761 aaggctgcgc acggcgtggc gggcgtcagc agtacatcgc aggcggcgaa ggcttgcgaa
     5821 aaatcgcgcg cgatcagggt ccgtactttt tgcgcctgca aataataggc gtcgtaataa
     5881 cccgccgaaa gcacataggt gccgatcatg atgcggcgct tgacctcacg cccgaatcct
     5941 tcggcgcggg tccgttcata gaggccgatc agatcgtcca cgcccggagc ccgatggccg
     6001 tagcgcacgc cgtcatagcg agcgagattg gaagaggctt ccgccggcgc cacgatataa
     6061 taggccggaa gcgcgtattt cgtgtgcggc aggctgacct cgacgatctc ggccccttgt
     6121 gccttcagcc aatcgatgcc gcgttgccac aatgcgtcga tttccggcgg catgttgtcg
     6181 atgcggtatt ctttcggaat gccaacccgc aaacctttga cgcccgcccc caacattggt
     6241 tcgtaatcgg gcaccggcac gtccacgctg gtggaatccc tcggatcgtg acctgccatc
     6301 acaccaagca tgatggccgc gtcctgcaca gtacgcgtca ttggccccgc ctggtcgagc
     6361 gaggacgcaa acgcgacgat gccccagcgc gagcagcggc cataggtcgg cttcatcccc
     6421 acaatgcctg tcactgccgc cggctggcgg atggagcccc cggtatccgt gccggtcgca
     6481 ccgagcgcca gatgcgccga cactgcggcc gccgaaccgc ccgacgaacc gcccggcacc
     6541 agatcctcat tacgcccatg cgcgcggaac gggttggtta ccggcccgaa ataggaagtc
     6601 tcgttcgatg agcccatggc aaattcgtcg agattggtct tgcccagcat gaccgcgccg
     6661 gcgttccaca gattctgcgt caccgtcgat tcataggtcg ggacgaaatt atccagaatg
     6721 cggctcgcgg cggtggtgcg cacgcctaca gtgcagaaca aatccttgat ggcgagggga
     6781 ataccttcga gcacccgcgc ttggcccgtg gcaatgcgcg catcggagga ttcggcagcc
     6841 gatagcgcat gttccggcgt ctcggtgacg aaagcattca gcgcccgcgc cttggcaacc
     6901 gcctggacat gagcgtcaac aagttcgctc gcggaaatat ctttcgccgc gagcgcctcg
     6961 cgcgcctggg caatggtgaa ttgcgtggga tcggaggggc gcgccatcac tccaccactt
     7021 tgggcacgac gaagaaattg tcttcggact gcggcgcgtt cttcatcaga tctgcggcgt
     7081 aaccgccgtc ggtcacgata tcctcgcgca tcttgagctt ctgttcgacg acactcgtca
     7141 tggggtccac gcccgtgacg tccacctcgg aaagctgctc cacccaggag aggagcgcat
     7201 tcaactcggt ttccatcgcg gcagtttggt cttcgtccac cgccagccgc gcaagccggg
     7261 cgatgcgccg gacggtctct ctatcgacgg acatgggccg cttctcgcgc caagtttctg
     7321 ataggaatgt tgctagcaat ggggcgcgga actggcaagc aatgacttgc ccaatgcggt
     7381 gtcgcgccaa ggcaaaggaa taggaatggc cgtcgtcgat ttggaccaat ttcgctcgca
     7441 tctgcgggcc ggccagcggc tccttgggct cgatctcgga gaaaagaccg tcgggctggc
     7501 gctttcggat acctctctta ccgtcgcgac gcccctgaag acgctcgccc gcggaaaatt
     7561 caggaacgat gccacgtcga ttgccgtgct gatcgtagag catgacatcg gcgggctcat
     7621 tgtgggtctg ccgctcaata tggacggaac cgacggaccg tcggcgcaat ccgcccgcgc
     7681 cttcgcccgc aatttcgctg ccgtttccga ctgtccgatt tgcctggtgg acgagcgcct
     7741 ttccaccgcc gccgtaacgc gcacgctcat cgacgccgat gcctcccggc gccggcgcgg
     7801 gcaagtcgtg gacaagatgg ccgcagccta tatcctgcaa ggcgcgctcg accggctgcg
     7861 caacagccac cctagggatt aagcgcggcg cggatttttg ccgcgatggc ctcgcgctcc
     7921 tcgtcgtccc tgtagcctct gcgtttccag atcagtcccg tggagaagag accgtcgccc
     7981 ttgcgccgcg gcacgatgtg gaaatgcaca tgcggcacgc tctgactgac cacattgttg
     8041 agcgccacaa agcttccttc gacccccagc cctatcatca cggcagcgga cagccatttc
     8101 acccgaaccc cgagccctgc catgacttcg tcagacgtct cagcaagggt ctcataatgc
     8161 ccacggggca ccagcaagac atgccccggc atcagcgggc gataatccag gaatgcgacc
     8221 gaaaaatcgt cggcgaagac cacaaaggct ttcccggtcc ctgccgcgat ttcgcagaac
     8281 tggcaaggtt tcggccggag tttggcggct ggcatgcgca actcctcatt tttacgaaaa
     8341 aatgtacagg tttctggtgg cccagccagc ggaacgcttt gcttcctggc cccacccgcc
     8401 tataagccca actttgatgc agaccctgcg gcacgtcacc tcgatcaacg atctcagcct
     8461 gccagagatc gaaacggtct tttcgctcgc ccagagctat ctgcggaacc tgcgtgatcc
     8521 gaatctgccg caccggatcg gacgcagcat cagcgatgca caaggctcgg tgttggcaag
     8581 cctgttttac gaacccagca cccgcacccg cctctccttc gaaagcgcca tgctacggct
     8641 cggcggcaag tctatcagca gcgcggatcc tgcgaccagt tccgccgcca agggcgaaag
     8701 cctcgccgat accgttcgcg tcgcctcaaa ctatgccgat gtgctcgtca tccgccaccc
     8761 tcgggacgga gcggcgaagc tcgccgccac ctatgccagt gtgccggtga tgaatggcgg
     8821 cgacggcgcg cacgaacacc caacccagac gctgtgcgat cttttcacgc tgctgcagga
     8881 aggcaagaac ctgaagcatc tgaatgtcgc gatttccggc gacctgaagg gcagccgtac
     8941 tatccactcc tttgtctacg ctctggcgcg tttcggcgcg aatatcatga tgatgccggc
     9001 taagggcatg gaactgccgt cccatgtcga ttggcgtttg cgcaatgaac tcgggtgcta
     9061 tgccgtgcca cgcgcgcgct acgagaaaga caatgcttcg gtcgatgcgc tctatatgac
     9121 ggcggacaag ccgcatcagc tttcgctgat ctccaatgtc gcgctcaagg tccgcaagaa
     9181 catcgatgcc atgtacgtca cgcgcttcca gaaggaacgc tggacggaca agacccagga
     9241 ctatcccacc atcgaccagc gcttcctcaa ggataaaaaa ttctcgaaga ccagcctgct
     9301 gcatccattg ccgcgtgtgg gtgagctcga catctcattg gataacgacc gccgcgcggc
     9361 ctatttccgc caggcggcct tcggcgtgcc catccggatg gcgctgatcg cgctgatcct
     9421 cggaatgggc cgcgactacg cgttgaagca atatcccggc gggttcaagc ccgacgaaca
     9481 tccggtctat gaccagccca acgataccgg aatcacctgc gccaacacga actgcatcgt
     9541 ccacgacccg caggagggaa aatatgcgcg caaccgtttt catctcgtag gcgcgaaccg
     9601 gcttagatgt ttctattgtg aaaccgacat cgtggatttc gtcgtcgccc acaaaaaatc
     9661 gaagcattac gaaaccgacg tttcgaaact gcatgccttt gccagcaagg atcgggacat
     9721 cgtgatctat ccccaagccg cggaagccga ggctgccggt ttctcgcttc ccaagcgccg
     9781 ccgcaaaacc gccggcggat aggatgatcg aaacgcttgg gctggtgcct tgggagacgg
     9841 cgtggcccta tatattcgcc acattttttg cctcgtactt tctgggatcg atcccctttg
     9901 gactcatctt cgcccggatt gcgggtgcgg gcgatatccg caaaatcggg tctggcaata
     9961 tcggtgccac caatgtgctg cgaaccggca aacgttgggc cgccgcggcg acgctctttt
    10021 gtgacggtgc caagggattt cttgcggtct ggtatgccgg tgaaaaattc ggcatccaat
    10081 tctttccggt gctggcgggg ttcggcgtct tcctaggaca cctctatccg gtctggctcc
    10141 ggttcaaagg cggcaaaggc gttgccacat tcatcggaat cgtcttcgcg cttgcctcgc
    10201 cggtcgggct gctggtctgt gtaagctggc tcggcctcgc gcaaatcttc cgctactcat
    10261 cggtggcggc gcttatttcc accgcactga cgccgtttta ctttcttttc ctgggcgcga
    10321 ctttgttcgc gctgctgacc ttcatcttgg ttgccctgat cttcatttcg catcggacca
    10381 acatcggccg cctgatcaga ggcgatgaac cgaagattgg cgcccgatga tgcggccccg
    10441 ccctctgcgc agcgatgaac ggcgcgcctg gcttcgactc gcgcgcaccg agaccatcgg
    10501 tccggccacc ttcgcagcgc ttatgtcgcg cttttccgat gcgcgcgaag cgctcgacgc
    10561 ggcaccgcgc cttgcccgac gcggcggagc ccaacaattg cgcatgccga gcgacgcgga
    10621 cgcagaggcg gaaatcgaag cgctgacaaa tcttggagga cggcttctgg cctgcgtgga
    10681 accggattat ccctccgggc tcgccgcact tgatccgccg cccccgctca tttcggtgct
    10741 gggccattcc gcgctgctca gccgcgacat ggtcgcaatg gtgggagcgc gcaacgcttc
    10801 ggcgctgcgc atcaagttcg cggcccgtct ggccgccgat ctcggcgcag ccggtctcgt
    10861 ggtcgtttcg ggtctcgcgc gcggcatcga tcatgcggcc catcgcggcg ccctggaaaa
    10921 cgggacggtt gccgttctgg ccggcggcgt ggatatcgtc tatccccccg agcatcaggc
    10981 gctttatgag gagatgaaga cccgtggcgc gctggtgtcc gaaatgccgc ttgggatggc
    11041 gccggtggcg cgccatttcc cccgccgcaa ccggctgatc tccggcatgg cgcgtggcgt
    11101 ggtggtcatc gaggccgccg aacgatcagg ttcgctgatc accgcgaatt acgcgctcga
    11161 acaagggcgc gaagttttcg ccgtgcctgg ctcgccgctc gatccgcgcg ccaaaggcgc
    11221 caaccggctg attcgagaag gcgcgacgct taccgagggt gcggacgatg tgctggccgt
    11281 gctgagacct atcctcggtc atgcatttca agaaccatcc gagcgtgccg tctcgccgcc
    11341 gtccgtcgac gaaattgccg ttgagcgcga agccgaccgc gtgcgaatcc tggtcgagga
    11401 gaaacttggg ccttctccgg tggaggtcga cgaactgatc cggcagtgcg gcgcgcctcc
    11461 ggcagccgtg ctgaccgtgc tcctcgaatt ggaattggca ggacgcgcgc aaaggcagcc
    11521 gggaaaccgc gtctcctggc gttagcgtga gctttcgcct gccttgacgc gccgcgcccg
    11581 gaagaaatca cgcaacaggg ttcccgcctc ctccgcgccc caaccccgct cgatgaccgg
    11641 gcgatgatga caggtatcca gcgcgaagaa tcgtgggcca tgctcgaccg ccccgccttt
    11701 cgggtccgga gccgcgtaga caagccgccc cacgcgcgca aggctgacag cccccgcgca
    11761 cattccacaa ggctccaggg tcacataaag cgtcgtcccg acaagccgtt cgttgccgat
    11821 ccggcgcgcg ccctccttta tcgccagcat ctcggcatgg gccgtcggat cgcgtatttc
    11881 aaggattctg ttgcccgata gcgcgagcaa atcatcctcc gccgacacga gcaccgcgcc
    11941 gaccggcacc tcgccccgct cgcccgccgc tttggcctcg gcgagcgcca tgcgcagata
    12001 ttccacgtct tgctctttca tggcgcgcag gatacccgaa agcgatgacg aaaacagacg
    12061 aagtgccgga actcgaacgc ggcgaacgca tcgccaaggt gatcgcgcga gccggtgtct
    12121 gctcgcggcg cgatgcggag aggctgattg ccgaaggacg tgtcgcgcta aatggcgaaa
    12181 agatcgcttc accggcgctc aacgtgcccg ccgatgcgct gatcacggtg gacggtaagc
    12241 ccatcccgac ggcatctccc accaaattgt ggcgctatca caagcctgct ggactggtga
    12301 catcgcatcg cgatccgcaa gagcgcgcga cggtattcga aaaattgccg cgctccatgg
    12361 cgcgtgtgat ctcggttggg cgtctcgaca tcaactcgga agggttgctg ctgctcacca
    12421 atgacggcgc gctcgcgcgt caactcgagc ttccctcgca agggtgggtg cgccgctacc
    12481 gcgtgcgcct gttcggcaag gtcactcagg gcgacctcga caggctggcc aaaggggtca
    12541 ccatcgacgg cgtgcgctat gaaccggtga cggcggaatt agaacgttcc aaaacctcta
    12601 acgcgtgggc gatggtcagc ctcaaggaag gcaagaaccg cgaggtcaaa cgcctgatgg
    12661 aatttttggg cttcaaagtc tcacgcctga tccgcgtcag cttcggaccg ttccatctcg
    12721 gccatctgcc cgaaggcgcg gtcgatgaaa ttccgggcaa ggtgtggcgc gagcaattgg
    12781 ggctgaccgg gaaaggcaaa gccgaacgcg aagagcggcc ggcgggagcg cgcacacgcg
    12841 gccggggcaa gacgcgcgct ccataagtca ggggaacaaa tgctccgtgg tccacggacc
    12901 ccgcggagcc tcacggcgat aacggatgcg ttcatgcagc cggaaaggac gatcgcgcca
    12961 gaattcaatg accacaggcc gcagccgaaa acccgaccaa tgggtggggc gaggcacggt
    13021 ggacagcgcg aatttcgcgg cgaagaacgc cacttctttc tccagggcaa agcgtccttc
    13081 caggggacgg gattgccgcg acgcccaggc gccgatctga ctgtccttcg gacgggtcgc
    13141 gaaataagcg tccgcctcgg tatcgctgac gggactcact ggcccgcgaa cgcgaatctg
    13201 ccggcgcagc gatttccaat gaaaacaaag cgcggctttg ggggtggccg ccagctcctc
    13261 gcctttggcg ctctcaagat tggtgtagaa gacgaagcct tcgtgatcgg cctgcttcag
    13321 cagcaccatg cgcacatcgg gcagacccgt cgcatccacg ctcgcgagcg acatggcgtc
    13381 aggatcgttc ggttcttgtg ccttggcctc tgcgaaccat tcgccgaata tctcgaacgg
    13441 ctcggcggat ggcttgactt cgccttcagc ctcagtcgcg ctcacttgta cagccggtct
    13501 atcgcaccgc tcttgtccag ggcggcaatg aagctcgaat cgtagaaatc ctcggctttg
    13561 tatttgcgca tttgcggcga atcatagatc gcgagcgtac tcttaatgcc ttcaactgca
    13621 ggatatggct tgcgtggaat atccaccgcc tcacggtaca tgctttgctg agttggcgta
    13681 tccttgatat tgaaccattt cacgagcgcc gcatcgaaag cgcctctatt tgttttcatc
    13741 aacgcatccg cttccagcgc ggctttgaca aaacgcgccg ccgtgtcccg attggctgcc
    13801 agccagttct tttcggcgag gatcgaggaa ccggcaacgg gaattttgta ttgggtcaaa
    13861 tcgatcaaat ccttgaactt gaggtcggac gccctggaga acagcatccc ggacccaagc
    13921 agtgcatctt cgcgcccctc gcgcaacgga ttgagtgcgt tgcctcctcc aacaagcgtg
    13981 atgtccttgc ccggctccca ccccatgacc tttgcaaagc caaccgcacc gacatgagtc
    14041 acggcgccgg ggaccgagta gccgagccgc tttcctttca aatctgccac ggacttgata
    14101 tcggtcgagg cgatgatgtg gtcgcggatg atggattcgc tggtcgcgag aacaatgcgg
    14161 tgcacaccac ctccattggc cacgcgatag atcatcgggc tgccgccgcc gatcgatatg
    14221 ggagcgtcgc cgatgtcggc tttgacgttt tcggccggca cgacgatacc ggagttgcgc
    14281 gcaacctgcg ccgcacccgg tgtgatgaat tggtgcacat cgagaccgtt cttggcgtag
    14341 atccccgcat ccgcggcaat cagaaacgac actttgttca gtgagacatc gcccagcgcc
    14401 acatcaagag acacgagctt gccggccgcc caggccggca gagccgcaag gcacaacagc
    14461 agaccgggta ggactttgcg cccaaaccgc gaaaccacca tgtttacctc ctgacggtgt
    14521 ttctgccgct catcataccg gataggagtg gatgcgacag ggcttacctt gaagggcaga
    14581 ctcttcctat ataggacgtc aaaggaagca aaaatgcgcg atccatacga agttctgggc
    14641 gtttccaaga aagcgaccga ggccgaaatc aagaaagcct ttcgcgcgct cgccaagaag
    14701 tttcatccgg acacgcatgc caacgatctc aaggccgtga agaagttcca ggaaatcagt
    14761 gccgccaacg agatcgtcgg ggacaaagag aaacgtgcgc aatatgaccg tggcgagatc
    14821 gatgccgcag gccagcccaa aggttttagc cagggtcatc atcctggcgg cttcgaacag
    14881 ggttttggag gtttccgcgg acgccccggc ggcggagcgg gaccccaggg cttcgaatat
    14941 gaattcacag gcaatggtcc ggaaggcttt caggcggacg acatattcgc cgacattctt
    15001 ggcggcctcg gcggcggagg aagacggcgc agtgcccaac cgcgaaaggg caacgacgtc
    15061 cagcttcaga caacggtctc gcttcaggaa gcggctttgg gcggaaccag acgcgtcgtg
    15121 ctcgccgatg gccggcagct cgaggtgcgg attccgccag gcgtcaagga gggccagcag
    15181 attcgcctcc gtggccaagg cgcgcctggc gaacgcggtg gcgtggcggg cgacgcgctc
    15241 atcaccattg ccatcgctcc acatccctat tttgagcgcg acggccgcga tttgcggctg
    15301 gatttgccga tcacgctcaa agaggccgta agcggggcaa aggttcccgt tccaactctg
    15361 acaggtcccg tgacggtgac ggttcctcct cattccaaca gcggtcgcgt attgcgtttg
    15421 aaagctaagg ggctgcccgg tacggggggc gatccggccg gcgatcttta tgcgcggttg
    15481 gtgatcacat tgcccgaagc aagcgatcca gagctcgatg atttcgtcaa ggactggaaa
    15541 cacgattacg atccgcgggc gaaattgaaa tagttcctct ttcaaggggt ggagttttgg
    15601 tgtgccgctc cgctatagga tgcaggcacg agagaggacc ggtgcatggc agttcctggg
    15661 atcatgacgg gcaaacgcgg gctcatcatg ggcgttgcga atgatcgctc tatcgcctgg
    15721 ggaatcgccc agtcgctcgc agcccagggt gcggagctcg ccttcaccta tcagggagac
    15781 gctttcaaga agcgcgtggt gccgctggtg gagccgctaa gccccgcagc cctgatcgat
    15841 tgcgatgttt cgcaaccagc ctcgctcgat gcggcatttg ccgaactatc ggcaaagtgg
    15901 gaccgccttg atttccttgt ccatgccatc gcgttctcgg acagagacca gctcaaaggc
    15961 cggtatgtcg acaccacggc tgaaaatttc accatgacca tgaatatctc ctgctactcg
    16021 ttcacggcgg tggcgaatca cgccgagaag atgatgacga acggcggcag ccttttgaca
    16081 ctcacctatt acggcgccga aaaagtgatg ccacattaca atgtgatggg cgtggcgaaa
    16141 gcagctttgg aagccagcgt gcgttatctt gccgaagatc tcgggaagaa gaacattcgt
    16201 gtcaacgcgc tctcggctgg gccgatccga accctcgcag cttcgggcgt gggcgatttt
    16261 cggtacatcc tgaaatggaa cgagctgaac gctcccctgc ggcgtaccgt cacgacggcc
    16321 gaagtgggcg attcggcgct gttcctgctc tccgatctct cgcgcgggat taccggcgag
    16381 gtaatgcatg tcgattcggg ctatcacatc gtgggtatga aagccgaaga cgcgcccgac
    16441 attgcggtcg tcaggtccga tggctcttga gctccaacag ggcgtgacac tgcacttcgc
    16501 gcgccatggc gaaaccatcg ccaatgtcga gcatcgtttc cagggacgca acgatacgcc
    16561 gcttaccgac cggggccgcg agcaggcgca caccatagcg gccattctct ccgactccct
    16621 tgctggaaac ccaacaccgc gattcgtcgc cagcccgctt ccgcgcaccc gtaccatgat
    16681 ggagatcgtt ctggatgcgc tccatctgcc ccgtgtctat gacacggatc cgcgcctcat
    16741 ggagatcgat ctcggggcct ggagcggcct gaccgatgcc gaagcctgcg cgctcgatcc
    16801 cgaaatgtgg gagcgacgga tccacgacaa atggaatgtc cgcgtgcccg gcggtggcga
    16861 gaattatgcg atggtggccg aacggctcac cgcctgggcg aaagaggtga accgcgatac
    16921 ggtcgcgatt tcgcacggtg ccgcgacacg catcctgcgt ggccttttac tcgggcttgg
    16981 ctggaaggaa atgtccgatc tggatgagcc acaggattgc gtgtttcgtt atcgggacgg
    17041 cacccttacc cggttggaaa accacgcccc gccgcccggc ccggtttgac gggctgggcc
    17101 acgttgccta aaaacggcag acatgtcgta caatagcttc ggccatcttt tccgctttac
    17161 aacctttggc gagagccacg gtccggcgat cggctgcgtg gtcgatggtt gtccgcccgg
    17221 cattgcgctg accgaagccg acattcaggt ctaccttgac cggcgcaaac ccggccaatc
    17281 gaaatttgtc agccagcgca aagaacccga cgccgtgcgc atcctgtcgg gcgttttcaa
    17341 ggacgagcgc atggccgaac aggtgacgac gggcacgccg atcgcccttc tgatcgagaa
    17401 tgtcgacgcg cgctccaagg attatggcga tattcgcgac aaattccgtc caggccatgc
    17461 cgactacacc tattgggcca aatacgggtt ccgcgattat cgcggcggcg gacggcaatc
    17521 ggcgcgcgag acggcgtcgc gggtggcggc aggcgcggtg gcgcgaaaga tcctggacgc
    17581 gatgtatggc gcagtgacga tccgcgccgc tttgatccag atgggcacgc tgacaatcga
    17641 ccgcggccgc tgggattggg acgaggtcct gcgcaacccg ttcaacgcgc ccgacgcggg
    17701 cgccgtcgat ccgtggacgg attatctgga acgcctgcgc aagtccggct cctcctgcgg
    17761 cgcgattgtg gaagtggtgg ccgacgggat tcccgtcggt ctcggcgctc cggtttacgg
    17821 aaaactcgat gccgatctcg cctccgccat gatgagtatc aatgcggtca aaggggtgga
    17881 gatcggcgcg ggcatgggtg ccgccgcgct caccggcgag gagaacgccg atgagatgcg
    17941 caggggcccg gacggcaatc ccatcttcct ctccaaccag gccggcggga ttctcggcgg
    18001 catatcgacg gggcagcaga tcgtggtgcg tttctcggtg aagccgactt cgtccatcct
    18061 gacgccccgc aagaccatcg acgtcgcggg caacgaaacc gaaatcatga ccaagggccg
    18121 ccacgatccg tgcgtgggca tccgcgcggt gcccatcggc gaagcgatga tggcctgcgt
    18181 gctcgccgac catgcgctgc gtcaccgcgc gcagatgggc taattcagcg tgaatgtgta
    18241 ggtctgggcc agagcaggcg cgatggtctt gccgatataa acctgcggct ccgacggcgg
    18301 cgtggcgcct tccttcatct cgcgctccgc tacgcagtcc ttcaccttgt ccgcgagggc
    18361 ggaaaagttc tgcaccatcg gcagggtctc cagcacgcac tggtcgaaga aggtgtattt
    18421 aaggtcttcg ccgcatccga atgacgtgcg gtcgggacgc gaagccgtga ggatcatgcg
    18481 attgtcggcc cgcagaaccg gaatgaacat gccggagaaa cacgccgata tgatcgccac
    18541 ggtgggacga tcatggcacg aattgtcgat gatgtcgcca acactgcgga tcgcgacgac
    18601 ctggtcgccg accaagatgc ccaaaggcgc cccatgtgac gtgaaataca caaagcagcc
    18661 gccggtggcg gtctcggtca gcttctcgaa ccccgcataa atcgccgggc catccgagcg
    18721 cagcggcttg gtttggggat ccaactccgg gcgggcggaa tattgctgga tatggttcgc
    18781 ggcaaagccc atctgcttca gcgccgttac cagatcgcgc cgggcattat cgaacacagg
    18841 acttggcgcg ccgtcatgag cgcgaaaatc tcccgccacc acgagagctg cccaattgtc
    18901 gaacggtccc gccgccgagg ccggagccgc cagcaacgac aatgccgccg ccaagacaac
    18961 aagaaagaca cgagccaaca tcatcttcgt cccataccac ggaatcggca aatcccgcca
    19021 gcgctcacgc acgggcaaat gccgcgaaca attcgacatg gggcgaccag agaaattgat
    19081 ccagcggtac gacgcgcatg agccggtatc caccatcggc caggatgcgc gcgtcgcggg
    19141 aaaaactcgc ggggttgcag gagatataaa gcacttcgga gacggccgat tgcgccaata
    19201 tctcggcttg cgccacagca cctggtcgcg gcgggtcgag cacgacgcaa tcgaagcggg
    19261 caagctcgct cgcgagcagc gggcggcgga acagatcgcg cgtctccgcc gtcagattcg
    19321 ctttgccggc tttggcggct gccgtgagcg ccgcgatctg ggcctcggcg ctgtccaccg
    19381 cgtggacatc gcggccatcc gccaacgcga gcgcaaaggt gccacagccg gaaaacaaat
    19441 cggcgacatg acgcgcaccc tttgctccct cgcatacgag gtcctgtaag atacgcatcc
    19501 cttccttcgt cggctgcaga aaggattccg gcggcagcgc cacagaaaaa cgtcccatgc
    19561 gcaaaaccgg cgccgccgcc agagccacca attcgccatt ccaggaaagc cgcgccagtt
    19621 tcagcgacgc agccagctcc gacagcatca tcaggaaatc ggggtcgcgc gcgcgcttca
    19681 ccttcagcga aaggtctatt ccattgtcgg cagcggttgc atgcagctca gcggtttcac
    19741 cggcacgcag caggggcgcg agccgcagct tcaatggcgc gataaggctc gcgagttcgg
    19801 acgacagcac gggacattcg gcgatatcga caaggcgccg gctttccggc tcatagaagc
    19861 cgagcgagac ggcgtgctcg ttcgcgcgcg ccttgaacat cgcgcgtctg cgtgtgcccg
    19921 gcggcacagc gcgaatgtct tcgacgggca cgtcggtaaa cccgcgctgc ttaagcgcgg
    19981 tgacaaccaa atcgcgcttc caggccaagt aaggctcgcg cgccatcatc tgaagcgcac
    20041 agccgccgca gcggccgaaa tgccggcacg cgggctgcgc acgtgatgcg cccggcgtca
    20101 cgatttccag aagccgcccg cgcgcaccct cgcgcgcgat gcgaaccaca tcgccgggca
    20161 cggtataggg cacgaacaga ttgtccgtga tgacgccgtc tccggcgtga cccagccgca
    20221 ctatctccac ggtttcggtc atgggcgcga ggaggccaac caggataccc cccctccccc
    20281 cttttgaggc cgcacgcacc ccgatttttc cagtatgcga gcggcttttc cgtccgcgcg
    20341 cgcgatttta tttggtgcga atagaggtgc aattccggtg cgattgtgtg caatgcgtgc
    20401 catgaaaatg ggattcgagt ctgggtccac ggacaggatg taggacaccg ccgccccgat
    20461 tgcaccctcg cgccgtcacc cggcggcgcg gcgtgcttcc cgcgcttcgg tcttcggcgt
    20521 catgcccaat ttcacccgcg catcccgttc gcccgcgtcc gcaagcatcg cgattgcatc
    20581 gggcgagaaa tcgccgctcg aactgcccgc ggccgccgcg ctatgggtgc tttcgacgat
    20641 atcgggcacc acgatatagc ccttgttcgc gccggcaaat tcatcggtct tgcgcagctc
    20701 cgcccagcgc tcgtccgaat tgatgagatc catcagcgcc gcgacatgat tgaaccggcc
    20761 cagcagcttc aggtcagacc gcgttttgtt ggcaaacagg agattgagaa tccgctgact
    20821 gacctgcgcc atgttcttgg gaacatcgcc cgcattgggg aagagattga cgacgatgat
    20881 ctgccgcgcg ctctcgcatt ctttttgcgg attcatgcgt tcgatcacag cgccgagcgg
    20941 cgtgttgtcg aaaattccgc catcccaata gaatttttct ccgattcccg tcatcggaaa
    21001 attgggcggc aggctgccgc tggcaagaat atggtcgagg gtgagcggca tgtccgcgct
    21061 gtcgaatggg gtgatttcac ccgcttcgat atcggtcgcg gtcaacagca atcgcggccg
    21121 cgccgatgtg tccgcaaggg cgtccaggtc gatcagctcc gccagagtaa cgcgcaaggg
    21181 ggcggtgtcg taaaggctcg tccacgaggg gaacgcgaaa acatccagcc gtgggcggaa
    21241 aaacgcagga tcgccgaaca gcgacgcgta agggcgcaaa gccggcagca ggaagtccgc
    21301 acgcagcgtg accttcttcc aaaatgcttc cagagtgccg agcggatcgc cgttcttcgg
    21361 acgcgccagc aacgccgccg tgatcgcccc aattgagacg cccgcgatca aatccggtat
    21421 cgggccttca tcgctgtaaa gcgcgcgcgc cacaccaagc tcatacgcgc cgagcgcccc
    21481 gccgccttgc agaacgaggg ccttcagaac gtctttccgc atgtcatctc cgttcatggg
    21541 ccattgagct taacgaggaa gcctccgcgc gagaaggccg gccatccgcc gcgacgcgga
    21601 atggcacttg tgctaaatac atcggcagag ggatgttcaa cgcgcgcgca tctttgaggg
    21661 tctgcatgaa cgagaatgat tttcccccga gcaagcatgt cacgcggcgc atcgcactcg
    21721 gtgccgtggc cgccgccgca acggcagccg ccggtccggt gctcggtgtg accgcgccat
    21781 cggaaggctg ccgcatcggc ccggcgcccc atcccaaggg tccgctcgtg ttcatgaatt
    21841 acgaccagat cgaactcgat gctgcctatg accagagcgc ctatgcgccc ttgggcgcgc
    21901 agatccaggc gcggcgcagc accaacagcg aggaggtgcg ccggcggctt ggcgctccac
    21961 gccgcgagtc ctatggcccg accgagatcg agaagctcga tatcttccgc accacgcgcc
    22021 ccaatgcgcc tgtgtttatt ttcgcccatg gcggcgcatg gctcggcggc gatgcgaagg
    22081 aatatcattt cgcggccgaa aacatggtga cggccggcgc ccattacatc gcgctcgatt
    22141 ttctcgcggt aggtgcggcc aatggcgata tccgggtgat ggccgattct gtcaaacgcg
    22201 ccatcgcctg ggtttataag aacgcggcga gtttcggggg cgatcccaac catatctatt
    22261 tgtgcggcca atcctcgggc gcgcatctgt cgggcgtggc gctcatcacc gattggcaga
    22321 aggattttgg cctgcctgcc gacatcgtca agggcggtgt gctgctctcg ggcatgtatg
    22381 agatgaagcc cgtgcggctg tcggcgcgca gcaaatatgt gaaattcgac gacgccatgg
    22441 aagattccat gagcacgatc cgtcacatcg acaggctgca cacgccgatc acgttgctct
    22501 acggcaccaa tgagacgccg gaattccagc gccagagccg cgatttcgcg gccgccgtga
    22561 aagccgcggg caagccggtc acgctcatcg tcgctcccaa ccacaatcat tatgaattgc
    22621 aggagacgat ggccaatccc tatggctggg cggggcgcgc cgcactcgcg atgatgggaa
    22681 tgggcaaggc ctgatcccaa ttcatcgtcc tcgctggcga taaggaacga caatggctga
    22741 agatcgccgc gaagcgctcc ttaaagaata tggcgaggtg tcgagcaatt ttcggcttct
    22801 gaccgatatc cgattcaagc ttctggcttt cctccccgcc gccactgccg tggccaccgc
    22861 gctaaaaggc gatagcctca gcgccgcaag cgccgtgctc tccctgttcg ggctcgcggc
    22921 gaccatcgga ctcgtgacgt acaatttgcg aaacgatcag ctgtatgacg agctcgtcgg
    22981 acgcgcagcg ttcatcgaac gaagcctggg aattccggac ggtgcattcg cgaaccggcc
    23041 gcgcccgtgg ctaattttcc ggtttctgca ttgtatc
//
LOCUS       DASYEG010000169         2251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_52538, whole genome shotgun sequence.
ACCESSION   DASYEG010000169 DASYEG010000000
VERSION     DASYEG010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52538"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1726)
                     /locus_tag="VGM72_07755"
     CDS             complement(<1..1726)
                     /locus_tag="VGM72_07755"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4265196.1"
                     /translation="MNEDVLANALALRRAGKFSEAAAIYSAILREQPKQFEALHGLGL
                     LSYQAGQLVEAERLIGEAVSVKPDAVDALYNRGSLLAKLNRHQEAVGCFDSALAVAPD
                     YAEARGNRGAALMVLGGYAEAFADFDRLVTLRSGSPEGWYMRGVALRRLERFGEARAS
                     YTQALEIRSNFADALRSRAEVSLLLENFAEALTDAEQAGALDPKNAEAWLLRADALVQ
                     LGRRDEALASYDKAIGLKPGNLDAIYNRATNMLPLRRFNEAVQGLEEALRLNPDCPFA
                     RGSLVFGKLCESDWYNLEGEIAAVEVALGKGTPIVPFQALVLCPNEARTHEAARRFSA
                     LKFPPLPKRLWQGEIYRHDKIRLAYLSGNFHHHAVARLMAGIFEHHDRRRFEVTAISF
                     GPDDNSAMRMRMNRAFDCFMDVRGETVERTAHRLRDMEIDIVVDLMGYTEDCRPAILP
                     HRPAPVQVNYLGYPGTMGAEYIDYIIADETVIPADRSAHYSERIVRLPQSYLPGGGAR
                     AIAETIPSRAGAGLPEDGFVFCCFHHVYKIMPPMFDLWMSLLHQVEGSVLWLSQTSPN
                     AAANLRREA"
     gene            complement(1723..>2251)
                     /locus_tag="VGM72_07760"
     CDS             complement(1723..>2251)
                     /locus_tag="VGM72_07760"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265197.1"
                     /translation="VEGSVLWLLEDNAAAAANLRAQALEQGIAPERLIFAPRTTPPEH
                     LARQRLADLFLDTSPYTAHTTCSDALWAGLPVVTFLGPTFAARVAGSLLNAAGLPELV
                     TASPAEYEQLALHLARDGVALAAIKAKLAANRKDCALFDTARFTRNLEAAFLTMADRQ
                     RAGLAPQSFAVEGAS"
BASE COUNT          448 a          724 c          688 g          391 t
ORIGIN      
        1 cggcctcccg gcgcagattg gcggctgcat ttggactcgt ctgcgaaagc cagagcacac
       61 tgccctcgac ctggtgcagc aagctcatcc agagatcgaa catcggcggc atgatcttat
      121 agacgtgatg gaaacagcag aacacgaatc catcttccgg cagcccggcc ccggcacgcg
      181 acggtatggt ttccgcaatg gcccgcgcgc cgccgcccgg aagatagctt tgcggcagac
      241 gcacgatgcg ctcgctgtaa tgggcggaac ggtccgccgg aatcacggtt tcatcggcga
      301 tgatgtagtc gatgtattcc gcgcccatgg tgcccggata gcccaaataa ttcacctgca
      361 cgggcgcggg ccgatgcgga agaatggcgg gacggcaatc ctcggtgtat cccatcagat
      421 cgacgacgat gtcgatctcc atgtcccgca agcgatgcgc ggttcgttcc acggtttcgc
      481 cgcgaacgtc catgaagcaa tcgaacgccc gattcatccg catacgcatg gcgctattgt
      541 cgtcgggtcc aaaagaaatc gcggtcactt caaagcgtct gcggtcatga tgctcgaata
      601 ttccggccat cagacgcgca acggcatggt ggtggaaatt tccggacaga taagcgagcc
      661 gaatcttgtc gtggcgatag atttctcctt gccagagccg cttcggcaga ggtggaaact
      721 tcaaagcgga aaacctgcgc gccgcctcat gggtgcgtgc ttcattcgga cagagcacaa
      781 gggcctggaa cggcacaatt ggcgttccct tgcccaaggc gacttccacc gcggctattt
      841 cgccctcgag attgtaccag tcggactcgc agagcttgcc gaagacaaga gaacctcgcg
      901 cgaaggggca atctggattg agacgcaacg cctcctcaag cccctgcaca gcctcattga
      961 accgccgcag tgggagcata ttggtggcgc ggttgtagat cgcgtccaaa tttccaggct
     1021 tcaggcctat cgctttgtca tagctggcca atgcctcatc gcgccggcca agctggacaa
     1081 gcgcgtcggc gcgcagcagc caggcttcgg cattctttgg atcgagcgcg cccgcctgtt
     1141 cggcatcggt cagcgcctcg gcaaagtttt ccaggagaag cgaaacttcc gccctgctcc
     1201 tcagcgcgtc ggcgaaattc gacctgattt cgagggcctg ggtgtagctc gcgcgcgcct
     1261 ccccgaagcg ttcgagacgc cgcaacgcaa caccacgcat gtaccaaccc tccggcgagc
     1321 cggaccgcaa ggttacaagc cggtcaaaat cggcaaacgc ctccgcgtaa ccgccgagaa
     1381 ccatgagtgc cgcgccgcga ttgccccgcg cttcggcata atcgggcgca accgcgagcg
     1441 cggaatcgaa acagccgacc gcctcttgat gccgattcaa tttggccagc agcgagccgc
     1501 ggttgtagag cgcatccacg gcatcgggtt tgaccgacac cgcttcgccg atcagccgct
     1561 cggcctcgac aagttggcca gcctgatagc tcaggagacc gagcccatgc agcgcctcga
     1621 actgcttggg ctgctcccgc aggatagcgc tatagatcgc ggcggcttcc gagaattttc
     1681 ctgcgcggcg cagcgcgagc gcgttggcga gcacatcctc gttcacgaag cgccctccac
     1741 agcgaaactt tgcggcgcga gaccggcgcg ctgacgatcg gccatcgtga ggaatgcggc
     1801 ttcgagattt cgcgtgaacc gcgcggtatc gaacagcgcg caatccttgc ggttggccgc
     1861 gagcttcgcc tttatagctg caagtgccac cccgtcacgc gccaggtgca gcgcgagctg
     1921 ctcatactcc gcgggcgatg ccgtcaccag ctctgggaga ccggcggcgt tgagcagact
     1981 gcccgcgact cgcgcggcga aagtggggcc gaggaacgtc acaacgggca gccccgccca
     2041 gagcgcgtcg ctgcacgtgg tatgcgccgt atagggcgac gtgtcgagaa agagatcggc
     2101 caaacgctga cgcgcgagat gctccggtgg cgtggtgcgc ggcgcgaaga tcaagcgctc
     2161 cggcgcgatc ccctgctcca gcgcctgggc gcgcaaattg gcggccgccg cggcattgtc
     2221 ctcgagcagc caaagcacgc tgccctcaac g
//
LOCUS       DASYEG010000170        12634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3097884, whole genome shotgun sequence.
ACCESSION   DASYEG010000170 DASYEG010000000
VERSION     DASYEG010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 12634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12634
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3097884"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VGM72_07765"
     CDS             complement(<1..917)
                     /locus_tag="VGM72_07765"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY4265198.1"
                     /translation="MNLVGIDIGGTFTDLVGCIDGRIVTSKSSTIPADPTQGVADTIG
                     LAACDMARLSEVLHGSTVAINTVLEHKGALTALIATKGFRDVYAIGRGNRIEGFNLFF
                     HRPKPLVARDLTFEVTERVNAKGEVLIALDDSALVELTAALRRHRIEAVAICLLHSYA
                     NPAHEQRIAQHLRAAMPDLFVTSSHEILREYREYERTSTTALNAYVGPRVQTYLRRLE
                     TYLRGQFFGGKIQIMRSNGGVMSIALAQDQPVSMMESGPVAGMIGAGRLARDLKLETC
                     IGFDMGGTTAKTSLITHGAPAIDEGYVIGG"
     gene            complement(914..1753)
                     /locus_tag="VGM72_07770"
     CDS             complement(914..1753)
                     /locus_tag="VGM72_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4265199.1"
                     /translation="MSLSETASRKIPALRHRRREQFSTPARIAWTRRALVLALLIVWE
                     IWARLSGNLELIAPPSAIARALFTDILSDGQIRAAILLTLFEVTCAYGLAIAIGLAMG
                     LAIGATELARRSLFPIILLLYAIPQVVLLPLFMLGFGIGPAAKIAFGFSHGVFPIIVN
                     VVAGMRDVNPLYLRAAQSMGARRADIIRDVIFPHMVTSFFAGLRLAMTMTLLGVILAE
                     LYVSTSGVGYFTRLFAESFNPAPLYALIGILAIIAILLNEIVRAAERRFTRWKHRSSW
                     MRE"
     gene            complement(1756..2511)
                     /locus_tag="VGM72_07775"
     CDS             complement(1756..2511)
                     /locus_tag="VGM72_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4265200.1"
                     /translation="MKQRPHLTPRLIQFAFLALTIAAWFVIDTTGAISPLFLPPMESV
                     WRAFLALVQTAIFWSAVRVTLTTILQAYIIAILLGIPAGYLVTRSRFLTALLEPIVSG
                     LFAIPITLFFPLFILLFGIGTGSKIAYGAAYGFFPIALNTIAGFSNVNPRFLDAAQSM
                     GLSRLGTLRHVLFPAAFPVILTGLRIAFFISFASVLGGETISSVSGVGRNIALAAELM
                     EPARMFAWMAAVIATALALNALVSRLESRMRGS"
     gene            complement(2508..3365)
                     /locus_tag="VGM72_07780"
     CDS             complement(2508..3365)
                     /locus_tag="VGM72_07780"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4265201.1"
                     /translation="MNLAHTSRRSARAYEAPVAIAVRNISQRYGDADSPQSVVALDNV
                     SLDVRRGEFLCLLGPSGCGKSTLLNIIGGLVQASEGEVSVGGAVVRRPSPEKIAFVFQ
                     ESTLFPWYSVAENFSIAFKYRGIGRSEWRDRTMVALKSVGMTDFADHYPDQLSIGMRQ
                     RVNLARGIAAGTEILLMDEPFAALDEQSRMVLGEDLSILLAQTGKTIVFVTHSLAEAV
                     FLSDRIALMSARPGRVKSIIDVDEPHPRLPGFMLEQRFSDLRNACYASLHDEIRQAMT
                     ATGCAGSAR"
     gene            complement(3406..4389)
                     /locus_tag="VGM72_07785"
     CDS             complement(3406..4389)
                     /locus_tag="VGM72_07785"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4265202.1"
                     /translation="MRTSRRNLLAMFASGIACASVLPTPAPAQGLRKVRVGKAINSSF
                     PFAGLELGEKQGIWTSVGLALEISTFRGDGQLQQALAAGSVDFGFGSGPGMGYAAKGV
                     PAHAVAVVANRPANMALVVAKTSHVTAIDGLKGKRIGVSTAGSLTDWLARNIAVSRKW
                     QPSDIEIVPMGEMRTRLAAMRSGELTAAVTSVQEAYQIQDNGQGIVLGTFGDVVPHFH
                     THVVFARDVLIQKDPDLVRRFLKGWFTVAAYMRDHRVETVKSVAATLRVGEKIVDQTY
                     PIETGMMSFDGRFDPEALETIRHSLKDLQILDFLPETSKLYNGSFVPVRLN"
     gene            4583..6640
                     /gene="tkt"
                     /locus_tag="VGM72_07790"
     CDS             4583..6640
                     /gene="tkt"
                     /locus_tag="VGM72_07790"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY4265203.1"
                     /translation="MDPRENSINALAINTIRTLAMDAVETAKSGHPGTPMGLAPVVYT
                     LWQRFLRFDPENVSWPNRDRFVLSAGHASMLLYAVLHLAGVKAPGGAPAVTLDDIRTF
                     RQLGSHCPGHPEYRHTAGVETTTGPLGQGCGNSVGMAMAARFLAKHFNRPDFALFDYN
                     VYALCSDGDLMEGVGSEAASIAGHLSLGNLCWIYDNNNVTIEGHTDLAFTEDVAARFR
                     GYNWNVERVGDANDCVRLAEALENFRAGGDTPMLIIVDSHIGYGAPHKQNTSSAHGEP
                     LGEEEVRLAKRFYGWPEDAQFFIPDGVRAAFDEGVGTRGRDASADWYKRLDAYRAKYQ
                     TLADELDLMEQGGLPAGWERDLPQFPADAKGMASREASGKALNAVARHYPWLIGGAAD
                     LSPSTKTDMKDGGDFEGEKAGRNLHFGVREHGMGAILNGLAVSKLRPFGATFLIFSDY
                     MKPAIRLAALMQVPVIYVFTHDSIGLGEDGPTHQPVEQLTQLRSIPGLVTLRPGDANE
                     TVEAWRFAIASKRPCALVLTRQALPTLDRRRYVAASELARGAYILAGADNPEPDVILI
                     ATGSEVSLCVQAHGELAKEGIAARVVSMPSWELFEEQDQAYRDLVLPPDIGARLSVEA
                     ASPIGWDRYVGSSGARIAMTGFGASAPAKALFAHFGFTVEHVLGAARDQIQRHRESAA
                     NPA"
     gene            6576..7676
                     /gene="gnd"
                     /locus_tag="VGM72_07795"
     CDS             6576..7676
                     /gene="gnd"
                     /locus_tag="VGM72_07795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEY4265204.1"
                     /translation="MSWARRAIRFNAIANLPRTLHSLGHSLRGFDACSSDERNSMQIG
                     IIGLGRMGGNIARRLMQHGHNCVVYDARADAIHALEKDGARGAKNLEDFVRNLQPPRI
                     AWVMLPAGRITDETVTQLGAAMQTGDIVIDGGNSFYRDDIRRARALKEKGVRYVDVGT
                     SGGVWGLERGYCMMIGGDPEAVRHLDPIFGALAPGAGEISRTPGRAEGGTAERGYLHC
                     GPVGAGHFVKMIHNGIEYGLMEAYAEGFDILHQAGGDTRAEDERYDIDLADVAELWRR
                     GSVIGSWLLDLTAISFTEDSALAQYSGYVDDSGEGRWTLMAAIESGVPVDVLSAALYA
                     RFRSRDSASFGNKLLSAMRKQFGGHVEPKHGS"
     gene            7679..9214
                     /gene="zwf"
                     /locus_tag="VGM72_07800"
     CDS             7679..9214
                     /gene="zwf"
                     /locus_tag="VGM72_07800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY4265205.1"
                     /translation="MEGPQPAGPCAFVIFGASGDLTKRLLIPALYHLKRANLLPDDFA
                     LVGISRTPQSDEQFRDRLGKSLRELSQDPIDDGIWDWFAQRMHYMAGSLDDSATYERL
                     KTLLLKMDADHHSSGNYLFYMAVPAAEFAPIVQHLGAAGLAREDNGQWRRVVVEKPFG
                     NDLKSAKALNRVLLENLTEPQIYRIDHYLGKETVQNIMVLRFGNGLFEPLWNRNHIDH
                     VEITVAETLGVERRGRYYDATGALRDMVPNHLFQLLTLTAMEPPTCFEADATRNEKGK
                     VLDAIRRFTRDSARQNAVRAQYGRGSIQGKTVASYREEPDVDPNSVTATYVAIKLTIE
                     NWRWAGVPFYLRTGKALAARHSEILIQFKQAPLTLFHSTPVERLIPNDLTLNIQPEEG
                     ASLRFGAKVPGQTMQIGDVEMKFNYGDYFRAAPSNGYETLLYDCMMGDASLFQRADTI
                     ESAWGIIQPVLDNWTEDRVSALPVYAAGSQGPAEADALLQRDGRGWRPIRTPADKTPA
                     GPA"
     gene            9211..9942
                     /gene="pgl"
                     /locus_tag="VGM72_07805"
     CDS             9211..9942
                     /gene="pgl"
                     /locus_tag="VGM72_07805"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEY4265206.1"
                     /translation="MKRDIAQEVLSDADALAHRVADWLLDLALAAKDRFAVALSGGTT
                     PRATYAVLVTPFYRERFPWQRTHWFWGDERFVPIEDRRSNYRMAWETMLSRASIPEAN
                     IHPIPTANVTPQDAAAQYERALKTFYGSDRLDPQRPLFDVNLLGLGEDGHFASLFPGS
                     EALDERRHWTAAVVGAQPEPRITLTYPALESCRHAAFVVSGAAKSAMLRGLAAGDANL
                     PAGRFDPVGALHIFADEKAAGPGGS"
     gene            complement(9946..10977)
                     /locus_tag="VGM72_07810"
     CDS             complement(9946..10977)
                     /locus_tag="VGM72_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY4265207.1"
                     /translation="MTATSRSFANWLVSALALLLLLVFGRPFLVPLVFALLIWAVLNA
                     LADGLVQFKLPRWLAWVGAFLLFLGALSAVTRIIGVEATQFAAGAPGYVMTLEQFIET
                     RLAPLGLGVNVEDFFSRSDLAGLLTGLATSLGFSIFGLIEVLIYVGFLLAEQSDIGAK
                     FGRLQLDPVRKQESQLVMHTVARQIQSYLGVCTILSALMSVVSYALLVLLGVSFAAFW
                     ALLIFFLTYVPTVGAVAVVLPALMALVQFGTLGPALIIVIALGAVHFLLMNVAATLIL
                     GQSLNLSPFAIILALTFWGLVWGLAGLFLAVPMTAAIAIVCAHIEGFRWVAVLLAAPP
                     PRGTKPLVH"
     gene            complement(11122..11286)
                     /locus_tag="VGM72_07815"
     CDS             complement(11122..11286)
                     /locus_tag="VGM72_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HEY4265208.1"
                     /translation="MSVGTILLIILIIALLGGFSGLGGGPFYGTGYYGGGGLGLVLII
                     VLILVLLGRI"
     gene            complement(11403..>12634)
                     /locus_tag="VGM72_07820"
     CDS             complement(11403..>12634)
                     /locus_tag="VGM72_07820"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEY4265209.1"
                     /translation="FLRSLGPGLVTGAADDDPSGIATYSQIGAQFGYGLAWTMLFSFP
                     LMVVIQEVSARIGCVTGYGIAQNLRRHYTPWLLRGVVLLLILANVINLGADLGAMGAA
                     LGLLIGGPKLLYAALFGLVCTLLEIFMSYARYAAVLKWATLSLFAYVAVVFAAHVPWG
                     TALAGTLIPRLVFDAPHAMAMVAILGTTISPYLFFWQAAQEVEELHRRHAKALCIAPR
                     DAGPELTRIRTDTVVGMGVSNMIALFIIFATAATLNANGVTDIQTSAQAAEALRPAAG
                     VFAFALFAAGIIATGMLAVPVLAGSAAYGVSEMFRWTEGLDRRPREAKAFYATIAVAT
                     LGGVMLNVTSLDPVKALYWSAVVNGVLAAPLMAVMMVIAMNPRIMGRLTLPRPMLVAG
                     WLATGVMALASLAFFVL"
BASE COUNT         2584 a         3825 c         4020 g         2205 t
ORIGIN      
        1 ccgccgatga catagccctc atcgatggcg ggcgcgccat gagtgatgag gctcgtcttg
       61 gccgtggtgc cgcccatgtc gaaaccgatg caggtttcca acttcagatc gcgcgcgagc
      121 cggcccgccc cgatcatccc cgcgacgggg ccggattcca tcatggagac gggctgatcc
      181 tgcgccagcg cgatggacat caccccgcca ttggaacgca tgatctggat cttgccgccg
      241 aaaaattggc cgcgcagata ggtctcgagc cggcgcagat aggtctgcac gcgcggaccg
      301 acataggcgt tgagcgcggt cgtcgaggtg cgctcatatt cgcggtattc gcgcaggatt
      361 tcgtgcgagg aggtgacgaa gagatcgggc atcgcggcac gcagatgttg cgcaatgcgc
      421 tgttcatgcg ccggattggc gtaggaatgc agcaggcata tggcgacggc ctcgatgcgg
      481 tgccgacgca gagcggccgt caattccaca agcgccgaat cgtcgagcgc aatcagaact
      541 tcgcccttgg cattgacccg ctcggtcact tcgaaggtca aatcacgcgc cacgagcggt
      601 ttggggcggt ggaagaacag gttgaaacct tcgatgcggt ttccccggcc gatggcatag
      661 acgtcacgga agcccttggt cgcgatcagc gcggtcagcg cgcctttgtg ttccagaacc
      721 gtattgatcg caaccgtcga accatgaagc acctcggaga gacgcgccat atcgcatgcg
      781 gcgaggccga tcgtgtcggc aacaccctgt gtcggatcgg ccgggattgt ggaagacttc
      841 gacgtgacga tgcgcccgtc gatgcagccg acgagatcgg tgaaggttcc gccaatatcg
      901 ataccaacga ggttcattca cgcatccatg acgatcggtg tttccagcgg gtaaagcggc
      961 gttcggcggc gcgcacgatt tcattcaaca ggatcgctat gatcgcgaga atgccgatca
     1021 acgcgtacag aggcgcggga ttgaaggatt cggcgaacaa tctggtgaaa taccccacgc
     1081 cgctcgtaga gacgtagagc tccgcgagaa taacgccaag cagcgtcatg gtcatggcga
     1141 gacggaggcc ggcgaaaaag cttgtcacca tatgagggaa gatcacatcg cggatgatgt
     1201 ctgcgcggcg cgcgcccatg gattgggcgg ccctgagata gagcgggttc acatcgcgca
     1261 tcccggctac gacattgacg atgatgggaa acaccccgtg gctgaagcca aaggcgattt
     1321 ttgcggcggg cccgatgccg aagccaagca tgaatagcgg caacagcacg acctgcggaa
     1381 tggcataaag caacaggatg atggggaaca ggctgcgccg ggccaactct gtcgcgccga
     1441 tggcaaggcc catcgccagt ccgatcgcga tcgccaggcc ataggcgcac gtcacctcga
     1501 acagggtgag cagaatcgcg gcgcggatct ggccgtcgga taggatgtcg gtgaacagcg
     1561 cccgcgcgat ggccgatggc ggtgcgatga gttccaaatt gccggagaga cgcgcccaga
     1621 tctcccagac gatgagcagg gcaagcacga gtgcgcggcg cgtccaggcg atgcgcgcgg
     1681 gtgtgctgaa ttgctcgcgt cgccgatgtc gaagcgccgg aatttttcgg gatgcggttt
     1741 cgctcagact catccttatg atccgcgcat ccggctctcg agccgcgaca ccaacgcgtt
     1801 cagcgcgagg gcggtggcga tgacggcggc catccaggca aacatgcgcg cgggctccat
     1861 caattcggcg gcgagcgcga tgttgcgtcc gactcctgag actgacgaga tcgtctcgcc
     1921 tccgagcacg gacgcgaatg agatgaagaa ggcgattcga agccccgtca ggatcaccgg
     1981 aaacgccgcg gggaacagta catggcgcaa tgtgccgagt cgcgaaagcc ccatggactg
     2041 ggcggcatcc agaaagcgcg ggttcacatt ggaaaatccc gcgatggtgt tgagcgcgat
     2101 ggggaaaaag ccataggccg cgccataggc gattttcgag cccgtgccga ttccgaacaa
     2161 caggatgaac agcgggaaaa acagggtgat cgggatggcg aaaagcccgc tgacgatagg
     2221 ttccaataac gcggtgagaa agcgtgagcg cgtcaccaga tagccggcgg gtatgccgag
     2281 aaggatcgcg atgatgtagg cctggagaat cgtcgtcagc gtgacgcgga ccgccgacca
     2341 gaagattgcg gtctgcacaa gcgcgaggaa ggcgcgccac acggactcca tcggcggcag
     2401 gaacaaaggg ctgatcgcgc cggttgtgtc tatgacgaac cacgcggcga tggtcagcgc
     2461 gaggaaggcg aattggatca gccgcggcgt aagatgcggg cgttgcttca tcgcgcgctt
     2521 cccgcacagc ctgtggctgt catggcttgg cggatttcgt catgcagcga ggcgtagcac
     2581 gcgttgcgca gatcgctgaa gcgctgttcc agcatgaagc cgggcaggcg cggatgcggc
     2641 tcatcgacat cgatgatcga tttgacgcgc cccggcctcg cgctcatcaa cgcgatgcgg
     2701 tccgaaagaa acacggcctc agcgagactg tgggtcacga acacgatggt cttgccggtt
     2761 tgcgccagca atatcgagag gtcttcgccg agcaccatgc ggctctgctc atcgagcgcg
     2821 gcaaagggtt cgtccatcag cagaatctcg gtgcccgccg cgatgccgcg ggcgagattg
     2881 acgcgctggc gcatgccgat ggaaagctga tccggataat gatcggcgaa atccgtcatg
     2941 ccgacggatt tgagcgccac catggtgcgg tcgcgccatt cgctccgccc gatgccgcga
     3001 tatttgaatg cgatgctgaa attctcggcc acgctgtacc agggaaagag cgtgctctcc
     3061 tggaacacga aggcgatttt ttccggcgag ggacggcgca cgacagcgcc tcctaccgaa
     3121 acctcgccct cgctggcttg aacgagaccc ccgatgatgt tgagaagcgt gctcttgccg
     3181 cagcccgatg ggccgagcag gcagaggaat tccccgcgtc tgacgtcgag cgacacattg
     3241 tcgagcgcaa cgacgctctg gggcgaatcc gcatcgccat aacgctggct gatattgcgc
     3301 acggcgatgg cgaccggcgc ttcataagcg cgcgctgacc ggcgagaggt gtgcgctaga
     3361 ttcacagcgt cctcgtggcg ttttcccgcg atccggccgg aaacctcagt ttagccgaac
     3421 cggtacgaag ctgccgttat aaagcttgga cgtctcgggc aggaagtcca ggatctgcag
     3481 atctttaagg gaatggcgga tcgtttccag ggcctcgggg tcgaaccggc cgtcgaatga
     3541 catcatgccg gtctcgatgg ggtaggtctg atcgacgatt ttctcgccaa cccgcaaggt
     3601 ggcggcgacc gatttgaccg tctcgacgcg atgatcgcgc atataggccg cgacggtgaa
     3661 ccagcctttg agaaaacgtc ggacgagatc ggggtccttc tggatcagca catcgcgcgc
     3721 gaacaccaca tgggtgtgga aatgcggcac cacgtcaccg aatgtgccca gcacgatgcc
     3781 ctggccattg tcctgaatct gataggcctc ctgcacggag gtcaccgcgg cggtgagttc
     3841 gcccgagcgc atcgcggcca gacgtgtgcg catttcgccc atcggcacga tctcgatatc
     3901 cgaaggctgc cacttcctgg agacggcgat gttgcgggca agccaatcgg tcagcgagcc
     3961 cgcggtggaa acgccgatgc gttttccctt gagcccgtcg atggccgtaa cgtggcttgt
     4021 cttggcaacc accagcgcca tgttggccgg gcgattggcg accaccgcga cggcatgggc
     4081 cggaacgcct ttggccgcat aacccatgcc gggaccggag ccgaagccga aatcgacgga
     4141 gcccgcggcc aaggcctgtt gcaactggcc atcgccccgg aacgtcgaaa tctcaagtgc
     4201 taggcccacc gatgtccaga tgccctgctt ctcccccaat tcgagtccgg cgaatgggaa
     4261 ggatgagttg atcgccttac cgacgcggac tttcctcagg ccttgggccg gggctggagt
     4321 tggcaggacg gatgcgcacg cgatcccgga cgcgaacatg gccaaaagat tgcgccggct
     4381 cgtccgcacg tttcgttccc tacgatgttt atcgttgcgg gcgagatttg cagcttggat
     4441 gccgcgaagc aagcggaagc cttgaatgaa agctgcgtga cgaggcccta tagttttcga
     4501 gtgcgatgcc accgatccgt caatgcgacg ttatgcaaac gccgcatctg atcaagcggc
     4561 cctccacagg gaaagatgca gcgtggatcc gcgtgaaaat tccataaatg ctcttgcgat
     4621 caatactatc cgcacgcttg ccatggatgc ggtggagacc gcgaaatccg gccaccctgg
     4681 tacacccatg ggtttggcgc cggtcgtcta cacgctgtgg cagcgtttcc tgcgcttcga
     4741 tcccgaaaat gtgtcctggc cgaaccggga ccgtttcgtg ctttcggcgg gacacgcctc
     4801 catgctgctt tacgcggtgc tgcatctcgc cggcgtgaaa gcacccggcg gtgcgcccgc
     4861 ggtcacgctg gacgacatcc ggacgttccg ccagctcggc agccattgtc ccggtcatcc
     4921 cgaataccgc cacacggcgg gcgtcgagac gaccacgggg cctttagggc agggatgcgg
     4981 caacagtgtc ggcatggcca tggccgcacg ctttctcgcc aagcacttca accggccgga
     5041 tttcgcgctg ttcgattaca atgtctacgc gctctgcagc gatggcgacc tgatggaagg
     5101 cgtcggcagc gaagcggcat cgatcgccgg ccatctgtcg ctcggcaatc tctgctggat
     5161 ctacgacaac aacaacgtga cgatcgaagg tcacaccgac cttgccttca ccgaagatgt
     5221 cgcggcgcgg tttcgcggct acaactggaa tgtggaacgg gtcggcgatg ccaatgattg
     5281 cgtgcggctc gccgaagcgc tggagaattt tcgcgccggc ggcgatactc ccatgctcat
     5341 cattgtggac agccatattg gttatggcgc gccgcataag cagaacacct cttccgcgca
     5401 tggcgagccg ctcggcgagg aggaggtgcg cctggccaag cgattctatg gctggccgga
     5461 agatgcccaa ttcttcatcc ccgacggggt acgcgcggcg ttcgacgaag gcgttgggac
     5521 gcgcggacgc gatgcctcgg cggattggta caagcggctt gacgcgtatc gcgccaaata
     5581 ccagacgctc gcggacgaac tcgacctgat ggaacagggc ggattgcccg ccggatggga
     5641 gcgcgatctg ccgcagtttc ccgccgacgc caaagggatg gccagccgcg aggcgtcggg
     5701 caaggcgctg aacgcggtcg cgcggcacta tccctggctg atcggggggg cggccgatct
     5761 gtcgccctcg accaaaaccg atatgaagga cggcggcgat ttcgaaggcg aaaaagccgg
     5821 acgcaatctt catttcggcg tccgcgaaca tggcatgggg gccatcctga atgggctcgc
     5881 ggtttcgaaa ttgcgcccgt tcggggcaac cttcctgatc ttcagcgact acatgaagcc
     5941 cgcgatccgg ttggcggctc tgatgcaggt gccggtcatc tacgtcttca cccatgattc
     6001 catcggattg ggagaagacg gcccgaccca ccagcccgtg gagcaattga cgcaattgcg
     6061 ctccattccc ggccttgtga cgcttcgccc cggcgatgcc aatgagacgg tagaggcttg
     6121 gcgttttgcg atcgcatcga aacgcccttg tgcgctggtc ctcacgcgtc aggcgcttcc
     6181 cacgctcgac cgccgacgct acgtggccgc atcggagctt gcgcgcggtg cctatattct
     6241 cgcaggcgcg gacaatcccg agcccgatgt catcctcatc gccaccggca gcgaggtttc
     6301 gctgtgcgtt caagcccacg gcgaattggc gaaagaaggc atcgcggcac gtgtcgtcag
     6361 catgccctca tgggaattgt tcgaggaaca ggaccaggcc tatcgcgatt tggtgctgcc
     6421 cccggacatc ggcgcgcgtc tgtcggtcga ggcagcctct cccattggct gggatcgcta
     6481 tgtcgggtca tccggggcgc ggatcgccat gacgggcttc ggcgcgtcgg ctcccgccaa
     6541 ggcacttttc gcgcatttcg gcttcacagt ggagcatgtc ctgggcgcgg cgcgcgatca
     6601 gattcaacgc catcgcgaat ctgccgcgaa ccctgcatag tctcggacat tctctacgcg
     6661 gcttcgacgc ctgttcatcg gatgaaagga actccatgca aatcggcatc atcggcttgg
     6721 gacgcatggg cggcaatatt gcgcgccgcc tcatgcagca cggccataat tgtgtcgtct
     6781 acgatgcgcg ggcagacgcc attcacgcgc tggaaaagga cggtgcccgt ggcgcgaaaa
     6841 acctggaaga cttcgtacgc aatcttcagc cgccgcgaat cgcctgggtc atgcttccgg
     6901 caggccggat caccgatgag accgtgacgc agctcggcgc ggcgatgcag acgggcgaca
     6961 tcgtgatcga tggaggcaac agcttctatc gcgatgatat ccgccgcgcg cgcgccctga
     7021 aggaaaaggg cgtccgctat gtcgatgtcg gtaccagcgg cggagtctgg ggtctggaac
     7081 gcggttattg catgatgatc gggggcgatc cggaggcggt tcggcatctg gacccgatct
     7141 tcggcgcgct ggcgccgggg gcgggcgaaa tctcccgcac gccgggccgt gcggaaggcg
     7201 gcacggcgga gcgcggttat ctgcattgcg ggccggtcgg cgccggccat ttcgtcaaga
     7261 tgatccataa cggaatcgaa tacggcctta tggaagccta tgccgaaggc tttgacatcc
     7321 tgcatcaggc aggaggggat acccgcgccg aagacgagcg ctacgacatc gacctcgccg
     7381 acgtcgcgga actctggcgg cggggcagcg tcatcggttc ctggctgctc gatctgaccg
     7441 cgatttcgtt caccgaagac agcgcgctgg cgcaatattc gggctatgtg gacgattccg
     7501 gcgaagggcg ctggacgttg atggcggcta tcgaatcggg tgtgcccgtg gacgtgttgt
     7561 cggccgcgct ttatgcccgc ttccgctcgc gcgattccgc gagcttcggg aacaagctgc
     7621 tctcggccat gcgcaaacag ttcggaggcc atgtcgaacc gaaacatggt tcgtgaccat
     7681 ggagggaccg cagcctgccg gaccttgtgc gttcgtgatt ttcggcgcct cgggcgattt
     7741 gaccaagcgc ctcttgatcc cggcgcttta ccacctcaag cgcgccaatc tgctgccgga
     7801 cgatttcgcg cttgtcggca tttcacgcac gccgcaatcc gacgaacaat tccgcgaccg
     7861 tctgggcaag agtctccgcg agctgagcca agaccccatt gacgatggca tttgggattg
     7921 gttcgcgcag cgcatgcatt acatggccgg cagtctcgac gattccgcaa cctacgagcg
     7981 tctcaaaacg ctcctgctga aaatggacgc agaccatcat tcgagcggaa actatctttt
     8041 ctacatggcg gttcccgcag ccgaattcgc cccgatcgtg cagcatttgg gcgcagcggg
     8101 actggcgcgg gaagacaacg gccaatggcg gcgcgtggtt gtcgagaagc cattcggcaa
     8161 cgatctcaaa tcggccaagg ccctcaatcg cgtgctgctt gaaaatctca ccgaaccgca
     8221 aatctaccgg atcgaccatt atctcggaaa agagacggtg cagaacatca tggtccttcg
     8281 tttcggcaac gggctgttcg agccgttgtg gaacaggaac catatcgacc atgtcgaaat
     8341 cacggtggcc gaaacgctcg gggtcgaacg gcggggacga tattacgacg ccaccggtgc
     8401 gctgcgcgac atggtgccca atcatctgtt ccagcttctg acgctgacgg cgatggagcc
     8461 gccgacctgc ttcgaggccg atgcgacgcg caacgagaag ggcaaggtgc tggatgccat
     8521 ccgcagattc acccgcgact cggcgcgcca aaatgccgtg cgggcgcaat atggacgtgg
     8581 ttcgatccag ggaaaaaccg tcgcctccta tcgcgaggag cccgatgtcg atccgaattc
     8641 ggtgaccgca acctatgtgg cgataaagct caccatcgag aattggcgtt gggcgggcgt
     8701 gcccttctat ctgcggaccg gcaaggcgct tgccgccagg cacagcgaaa tcctcatcca
     8761 gttcaagcag gcgccgctca cgctgttcca cagcacgccg gtcgagcggc tgatccccaa
     8821 cgatctcacc ctgaatatcc aaccggagga aggggcctcc ttgcgctttg gcgcgaaagt
     8881 gccgggacag accatgcaga tcggcgacgt tgaaatgaaa ttcaattacg gcgattattt
     8941 ccgcgccgct ccgagcaatg gctacgaaac gctgctgtat gattgcatga tgggcgacgc
     9001 aagcctgttc cagcgcgccg ataccatcga gtccgcgtgg gggatcatcc agccggtgct
     9061 cgacaattgg acggaagatc gtgtcagtgc gttgccggtc tatgccgcgg gcagccaagg
     9121 tccggcggaa gctgacgcgc ttctccagcg cgatggcagg gggtggcgtc ccatcagaac
     9181 cccggcggac aaaactccgg cagggcccgc atgaaacgcg acatcgcaca ggaggtgctg
     9241 agcgatgcgg atgcgttggc gcaccgtgtc gcggattggc tgctggacct ggctcttgcc
     9301 gcgaaggatc gattcgccgt tgcgctgtcc ggtggcacga cgccgcgcgc aacctatgcg
     9361 gttctcgtta cgccgtttta ccgcgaacga tttccctggc agcgcacgca ctggttctgg
     9421 ggtgacgagc gttttgtgcc cattgaggat aggcgcagca attaccggat ggcatgggaa
     9481 accatgctgt cgcgtgcgtc gatccccgag gccaatatcc atccgattcc gaccgcgaac
     9541 gtcacgcccc aagacgccgc cgcgcaatat gagcgcgcgc tgaagacttt ctacggttcg
     9601 gaccggctcg atccgcaacg gccgctgttc gacgtcaatt tgctggggct tggcgaggat
     9661 ggccatttcg cctccttgtt tccgggttca gaggcacttg acgagcgccg gcattggaca
     9721 gcggcggtcg tgggcgcaca acccgagccg cgaatcacgc tcacctatcc ggcgctggaa
     9781 agctgccgtc atgccgcgtt cgtcgtctcc ggcgcggcga aatccgcgat gcttcgcggg
     9841 cttgccgccg gcgatgcgaa tcttcccgcc ggccgattcg atcccgtggg ggcgcttcat
     9901 atcttcgctg atgaaaaagc cgcgggcccc ggaggatcgt aaagctcaat gaacgagcgg
     9961 tttggtcccg cgtggcggtg gcgcggcaag caggaccgcc acccagcgga acccttcgat
    10021 gtgggcacag acaatcgcaa tggccgcggt catgggcaca gccagaaaca gcccggccaa
    10081 tccccacacg agtccccaga aggtgagggc gaggataatg gcgaagggac tgaggttcag
    10141 ggattgtccc aggatgagcg tcgccgccac attcatcaga agaaaatgga ccgcgccgag
    10201 cgcgatgacg atgatcagcg ccgggcccag cgtcccgaat tgcaccagcg ccatcaatgc
    10261 gggcagcacc accgcaaccg cccccaccgt cgggacgtag gtcaggaaga agatgagcaa
    10321 cgcccagaac gccgcgaaac tgacgccaag caacacaagc aaggcgtagg acaccacgct
    10381 catcagcgcg ctcaggatcg tgcacacgcc gagataagac tggatctgcc gcgcgacggt
    10441 gtgcatgacc agctggctct cctgcttgcg caccggatcg agctgcagcc ggccgaattt
    10501 ggctccgatg tcgctttgtt cggcaagcag gaacccgaca tagatcagca cttcgatgag
    10561 accgaagatc gagaagccca aggatgtcgc caaccccgtc agcaatcccg caagatcgga
    10621 acggctgaag aagtcttcga cgttcacgcc cagccccaac ggcgccagcc gtgtctcgat
    10681 gaattgctcc aatgtcatca cgtagccggg cgctcccgcg gcgaattgcg tggcttccac
    10741 gccgatgatc cgggtgacgg cggaaagcgc gcccaggaac agcaggaaag cgccgaccca
    10801 ggcgagccag cgcggcaatt tgaattgaac gagcccatcg gcaagggcgt tcaaaaccgc
    10861 ccatatcaaa agcgcaaaga caaggggcac caggaaaggg cggccgaaaa ccagcagcag
    10921 aagaagtgca agcgcgctta ccagccagtt cgcaaaactt ctgctcgtcg ctgtcatcgg
    10981 cgatttcgcc acattcgagg gcagacagtc atctgcaaaa ctcgcctcag cgaatcgaaa
    11041 aaacattccg agaacaatcg gtgtaagccg attcgtatgc cctttcgagt cgctgagctt
    11101 ttcgacgcgg aagctcggga cttaaatcct gccaagcagg accagaatca gcacgatgat
    11161 cagaacaagg ccgaggccgc cgccgccata atagccggtt ccgtagaagg ggccgccgcc
    11221 gaggccgcta aagccgccaa gcagcgcgat aataagaatg atgagcagta tagtgcctac
    11281 agacatggcg atttcccgat tggggtagct gtacacgctg cccggagcac cggctcacgc
    11341 ccggcagtct ggaaatgaat tgccgtggcc aaggttcccg aggaaaccgt gattcgcccg
    11401 gttcagagca caaagaacgc gagggatgcg agcgccatca cccctgtggc gagccatccg
    11461 gccacaagca tggggcgcgg gagcgtcagc cgtcccatga tacgcgggtt catcgcgatc
    11521 accatcatga cggccatcag cggggcggcg agcacgccat tgacgaccgc gctccaatag
    11581 agcgccttga ccggatcgag ggaggtgacg ttcagcatca cgccgcccag cgtcgcgacc
    11641 gcaatcgtgg cgtagaacgc tttggcctct ctggggcgcc ggtcgaggcc ttctgtccag
    11701 cggaacattt cgctcacccc ataggccgcg gacccggcga gcacggggac cgcgagcatg
    11761 ccggtcgcga tgatgccggc cgcgaacagg gcgaacgcga agacgccggc ggccggccgc
    11821 aatgcctcgg ccgcctgcgc agatgtctgg atatcggtca ccccgttcgc gttcagcgtc
    11881 gcggcggtgg cgaatatgat gaacagcgcg atcatgttcg acacgcccat cccgacaacc
    11941 gtgtcggtac gaatgcgcgt cagttccggc ccggcatcgc gcggggcgat gcaaagcgcc
    12001 ttcgcgtgcc ggcgatgcaa ttcctccact tcctgcgcgg cttgccagaa gaacagatag
    12061 gggctgatcg tcgtgccgag aatggcgacc atggccatgg catggggcgc atcgaagacg
    12121 agtcgcggaa tgagagtgcc agcgagggct gttccccaag gcacatgtgc ggcaaagacc
    12181 accgccacat aggcgaagag cgacagagtt gcccatttga gcacggcagc atagcgggca
    12241 taactcatga agatttcgag cagggtgcaa accaggccga acaacgcggc ataaaggagc
    12301 ttgggtccgc cgatgagcaa gcccagcgcc gcgcccatgg cgccgaggtc cgcgccgaga
    12361 ttgatcacgt ttgccaggat gagcaggagc acgaccccgc gcaggagcca aggggtgtaa
    12421 tgccggcgca gattctgggc gatgccatag cccgtgacgc agccgatccg tgcgctgacc
    12481 tcctggatca ccaccatcag cgggaagctg aacagcatgg tccatgcgag gccatagccg
    12541 aactgcgcgc ctatctggct gtaggtcgcg atgccgctcg ggtcatcgtc cgccgcgcct
    12601 gtgacgaggc cgggcccaag gctcctcaga aatc
//
LOCUS       DASYEG010000171         8263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4257650, whole genome shotgun sequence.
ACCESSION   DASYEG010000171 DASYEG010000000
VERSION     DASYEG010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 8263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8263
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4257650"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..441
                     /gene="fliQ"
                     /locus_tag="VGM72_07825"
     CDS             178..441
                     /gene="fliQ"
                     /locus_tag="VGM72_07825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEY4265210.1"
                     /translation="MTGAETLDFARESIYLLLELSAPTMLTALVVGVVIGLLQAMTQI
                     QEMTLVFVPKILAIFLVLLFALPMAGEAMATYMNNIAARIAQP"
     gene            438..1202
                     /gene="fliR"
                     /locus_tag="VGM72_07830"
     CDS             438..1202
                     /gene="fliR"
                     /locus_tag="VGM72_07830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEY4265211.1"
                     /translation="MNVILPQLQGVVLVYLLTFSRAGAMIMLLPVIGDAGVPARVRLA
                     FALAVSAALVSVTARYYPTNTPPPLELAALIAREATAGVLVGTMARLIMSTLETAGTL
                     IAVQTGLAFATTLDPGQGTQSAIVSTFLSLVGSLLVFETGLHHLAIGAISGSYTLLPP
                     NQAFPAGDMAEMTLNLVSGSFALGLQLASPFIVFGLLIYATLGVLSRLMPQLQIFFLA
                     MPVSILTGFVLLMLFLGVMMTAFLDFFAGQMRLFGG"
     gene            1209..2291
                     /gene="flhB"
                     /locus_tag="VGM72_07835"
     CDS             1209..2291
                     /gene="flhB"
                     /locus_tag="VGM72_07835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HEY4265212.1"
                     /translation="MAEDSDDSQRTEEPTQKRLDEALKQGDVVKSSELSSFVMLAGGT
                     LALALFAHNAATRFAADFTVFLASPGEMTLDAGAMTGLMKRCVFGFFAITGPAAGLML
                     IAALAGHLLQHRPFFSTEKIKPDLGKLSPVAGFKRLFGLDGIVNLVKGVAKIVLVGTA
                     AFLAVWPERSRLASALDMNPLGIASLSLTLIGKMLIAALIVLAAIAALDYFYQRQRFM
                     ARHRMTRQELKDEVKQSEGDPIVKARIRQIRLERSKKRMIAAVPGANVVIVNPTHYAV
                     ALKYESGKMAAPVCVAKGMDHLALTIRKVAEEHDVPIVENPPLARALYAAVDLDEPVP
                     PEHYKAVAQIIGYVMRLANKNRFWRN"
     gene            2319..4478
                     /locus_tag="VGM72_07840"
     CDS             2319..4478
                     /locus_tag="VGM72_07840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4265213.1"
                     /translation="MTGIVILLSRNFSPRAVGGTSRLAALLFFLLAVIGAGAILLEPQ
                     FARWAGVVLVGFIGVGAAFLLVAAWPRTSADLDDARRAAAAAASANVAWAVTAKDGAV
                     LDCNIAYRFLAGAGEGEPPAPPQLAFSGEAPAAALYRLARAAGEGRAREENFETESGQ
                     KLTAVVRALRGGDSAWWFTPLLPGAAPQRTVVEKKSPSGLTRFADFFRNAPIAVAITG
                     AEGAIAEANSGFMEFFALEANRVGARMETLVAEHERAAVIDLIARAGRGDVSQVPVEI
                     HSASNAKSPDRTAQLFGSPFMPGLGGEPCALLYIVDTSEQKALETQFAQSQKMQAIGQ
                     LAGGVAHDFNNLLQAIMGNCDLLLMRHPAGDPSFAEINEVRQNSVRAAGLVRQLLAFS
                     RQQTLQPKVLALSDTLTELSLLLRRLVGERIVLRVEHPQDLWPVRADEGQLSNAVMNL
                     VVNARDAMPPEGGAVTIRTSNVTFAGAEPIGTGHIPAGDYVLIEVIDTGSGIPKENLT
                     KIFEPFFTTKPVGQGTGLGLSTVYGVVKQTGGFITVDSERGQGTSFRVYLPRHTAPSA
                     PEVVEESERAAPRDLTGEDTILLVEDEDAVRSFAARALRMRGYTVLEASGGEAALDIV
                     RSRASTIDLLVTDVVMPNMDGPTLVRAARGLRPDMRVIFMSGYAEDAFRRNEEKVEEL
                     HFLPKPFGLKQLVAKVKEVLSEVPPAPKPPAPNDSGT"
     gene            4627..5700
                     /gene="recA"
                     /locus_tag="VGM72_07845"
     CDS             4627..5700
                     /gene="recA"
                     /locus_tag="VGM72_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEY4265214.1"
                     /translation="MAQAVLRVVAKEDSDKKRALEAALSQIDRAFGKGSVMKLGQREK
                     AVDADAISTGSLGLDIALGIGGLPRGRVVEVYGPESSGKTTLALHVVAEIQKKGGVAA
                     YVDAEHALDPGYAKKLGVDIDELLISQPDTGEQALEITDTLVRSGGVDIVVVDSVAAL
                     TPKAELEGEMGDQLPGLQARLMSQALRKLTGSISKSNTIVLFINQIRMKIGIMFGNPE
                     TTTGGNALKFYASVRLDIRRIGAIKDHDEVTGNQTRVKVVKNKVAPPFRQVEFDIMYG
                     HGISKSGELVDLGAKAGIVEKSGSWFSYDGQRIGQGRENAKLYLETNQDVAAAIEAAI
                     RSNAGLLSAGLTVPNDEPATAGD"
     gene            5985..>8263
                     /gene="alaS"
                     /locus_tag="VGM72_07850"
     CDS             5985..>8263
                     /gene="alaS"
                     /locus_tag="VGM72_07850"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY4265215.1"
                     /translation="MNSLKDIRATFLEFFHSCDHEVVASSPLVPRNDPTLLFTNAGMV
                     QFKNVFTGQEKRAVPRAATAQKCVRAGGKHNDLDRVGYTARHHTFFEMLGNFSFGDYF
                     KDRAISLAWDLITKDFGLSKDRLLVTVYAEDNDAFDLWRKIAGLPESRIIRIGTSDNF
                     WSMGEVGPCGPCSEIFYDHGDHIPGGPPGSPNEDGDRFIEIWNLVFMQYEQLAGGSRE
                     SLPRPSIDTGMGLERITAVMQGVHDNYDIDLFRHLIEASAEYSGTAIAGPHAVSHRVI
                     ADHLRSSSFLMADGVLPSNEGRGYVLRRIMRRAMRHAHILGSKDPLMYRLVPTLVAEM
                     GEAYPELKRAEALLSETLKLEELRFRDTLARGLKLLDEATSAMQPGDTLPGEMAFKLY
                     DTYGFPRDLTEDALRERGMKVDIAGFDFAMAHQKSEARKAWSGSGEAATDALWFALRE
                     EIGPTEFLGYDTEAAEGAILAIVRDGDRVQSGRAGETLRIITNQTPFYGESGGQAGDR
                     GAMFCAGGAEGTVIDTEKKLGNLYVHVVEITRGEFKVGNAVDLRVDTERRRATRANHS
                     ATHLLHAALKHVLGPHVGQKGSLVSADRLRFDFSHPKAMTPEELERVEDEVNQVIRQN
                     NDVSTRLMATAQAIEAGAVAMFGEKYGDEVRVLAMGQDLNAAAKPYSVELCGGTHVRR
                     LGDIQLFTILGESAVAAGVRRIEALTSEGARRYLESQGEAAREAAAVLKTSAVELPSR
                     IHALVEDRRRLERELSEAK"
BASE COUNT         1531 a         2488 c         2578 g         1666 t
ORIGIN      
        1 caccatgcac accggagaga tggccgcggc aaaaggcgcg accgggcagg gcgacgtggt
       61 ccaggttgtc aatgcggtga ccgcggccga actcaccttg cagaccgtgg tggcgatccg
      121 cgaccgcgtg atttccgcct atcaagacat catgaagatg ccgatttaag gaaccccatg
      181 accggagccg aaacattgga ttttgcgcgg gagtcgatct acctcctgct cgaactctca
      241 gcccccacca tgctcaccgc gctcgttgtc ggcgtggtga tcgggctgtt gcaggccatg
      301 acccaaatcc aggaaatgac gctcgtcttc gtgccgaaga ttctcgcgat ctttctggtg
      361 ttactgttcg ccttgccgat ggcgggtgaa gccatggcaa cttatatgaa taatatcgcc
      421 gcgcgcatcg ctcagccatg aacgtgattc tgccccaatt gcagggggtg gtgctggttt
      481 atctcctgac gttttcgcgc gccggcgcca tgatcatgct gctcccggtg atcggggacg
      541 cgggcgttcc ggcccgtgtc cgccttgctt ttgcgcttgc cgtctccgcc gcgctcgtgt
      601 cggtcacggc gcgctattac ccaacgaaca caccaccgcc gctcgaactc gcggcgctga
      661 tcgcgcgcga agcgacggcg ggcgttctcg tcggcaccat ggcgcgattg atcatgagca
      721 cgctcgaaac cgccggaaca ttgatcgcgg tgcaaaccgg acttgcgttc gccacgacgc
      781 tcgatcccgg ccaaggcacg caaagcgcca tagtttcgac cttcctctcg cttgtcggtt
      841 cactgctggt gttcgaaacg ggcctgcatc atctcgcaat cggcgcgatc agcgggagct
      901 acacgctgct gccgccgaac caggctttcc cggccggcga catggcggag atgacgttga
      961 atctggtctc gggctccttc gcgctcgggc tgcaattggc atcgccgttc attgtcttcg
     1021 gcctgctgat ctatgcaacc ctcggcgtgt tgtcgcgtct gatgccgcaa ctgcagattt
     1081 tcttcctcgc catgccggtg agcatcctca ccgggttcgt gctccttatg ctgttccttg
     1141 gcgtgatgat gaccgcgttc ctcgatttct ttgcaggcca gatgcgcctc tttggaggtt
     1201 gaaggttcgt ggcggaagat agcgacgact cccagcgtac cgaggaaccg acccaaaagc
     1261 gtctcgacga ggcgctgaag cagggcgatg tcgtcaaaag ctcggagctt tccagtttcg
     1321 tcatgctggc gggcggtacg ctggcgctgg cgctttttgc ccataatgcg gccacgcgat
     1381 tcgccgcgga tttcaccgtc ttccttgcga gcccgggtga aatgacactc gatgccggcg
     1441 ccatgacggg tctcatgaag agatgcgtgt ttggattttt cgccattacc ggtccggcgg
     1501 ccgggctgat gctgatcgcg gctctggcgg gacatctgct gcagcaccgt ccgttctttt
     1561 ccaccgagaa gatcaagccc gatctgggaa aattgtcgcc cgtcgcgggt ttcaagcggc
     1621 tgttcggact ggatggcatc gtcaatctcg tcaaaggggt ggccaagatc gtcctggtcg
     1681 gcaccgcggc cttcctcgcg gtctggcccg aacgttcccg gctggcttcc gcgctcgaca
     1741 tgaatcccct gggcatcgcc tctctgtcgc ttaccctcat cggcaagatg ctgatcgccg
     1801 ccttgatcgt gctcgccgcc atcgcagcgc tcgattattt ctaccagcgt caacgcttca
     1861 tggcccggca ccggatgacc cgtcaggaat tgaaggacga ggtcaagcag agcgaaggcg
     1921 atcctatcgt caaggcccgc attcggcaga tccgcctgga acgctccaaa aagcggatga
     1981 tcgctgccgt tcccggcgcg aatgtggtta ttgtgaaccc gacacactat gccgtcgcat
     2041 tgaagtacga atcaggcaaa atggccgcac cggtttgcgt tgccaagggc atggatcatc
     2101 tggcgcttac catccgcaag gttgccgaag agcatgacgt tcccatcgtt gaaaatccgc
     2161 cgcttgcccg cgccctttat gcggcggtcg acctcgatga gccggtcccc ccggagcatt
     2221 acaaggccgt ggcccagatc atcggctatg tcatgcggtt ggcgaataag aacaggttct
     2281 ggcggaactg accgcgcgcg cctcccgggg ctgcggccat gaccggcatc gtaatccttc
     2341 tgagccgcaa tttctccccg cgcgcggttg gcgggacgtc acgcctcgca gcgcttttgt
     2401 tctttctgct ggccgtcatt ggagccggcg caattctgct ggagccgcaa ttcgcccgct
     2461 gggccggtgt tgtgctggtc ggtttcatcg gagtgggcgc tgcgttcctg ctggttgccg
     2521 cttggccgcg cacctcggcc gatctcgacg atgcccgccg ggccgccgcg gcggcggcct
     2581 cggccaatgt tgcctgggcc gtgaccgcca aggatggggc ggtgctggac tgcaatatcg
     2641 cctaccgctt tctggcgggt gcgggtgagg gcgagccgcc cgctccaccg cagctggcgt
     2701 tttcaggcga ggcgcctgcc gccgcgcttt atcgtcttgc gcgggcggct ggcgagggcc
     2761 gcgcccggga agagaatttc gaaacggaat cggggcagaa gctcaccgcg gtcgtgcgcg
     2821 ctttgcgcgg cggcgattcc gcctggtggt ttacgccgct gcttcccgga gccgccccgc
     2881 aaagaacggt ggtggaaaaa aagagtccct cggggctgac ccgctttgcc gattttttcc
     2941 gcaatgcgcc catcgcggtt gccattaccg gcgcggaggg tgcaatcgcc gaagccaata
     3001 gcggttttat ggaattcttc gcgcttgagg ccaacagggt tggggcgcgg atggagacac
     3061 tcgtcgccga acatgagcgt gccgccgtca ttgacctgat cgcgcgggcg gggcgcgggg
     3121 atgtcagcca ggttcccgtc gaaattcact ctgcgtcgaa tgcaaagtcg ccggatcgca
     3181 cggcccagct tttcggaagc ccgttcatgc cgggtctcgg cggggagccg tgcgctctcc
     3241 tttatatcgt ggatacgtcc gagcaaaagg cgctggagac ccaattcgcc caatcgcaga
     3301 aaatgcaggc gatcggccaa ctggccggtg gtgtcgcgca tgatttcaac aatcttctgc
     3361 aggcgatcat gggcaattgc gatctcttgc tgatgcgtca tcccgcaggc gatccctctt
     3421 ttgcggagat caatgaggtc aggcagaatt ccgtccgggc ggccgggttg gtgcgtcagc
     3481 ttctggcttt ttcgcgccag cagaccttgc agcccaaggt gctcgcgctt tccgatactc
     3541 tgacggaatt gtcacttctg ctgcgccggc tggtgggaga gcgaattgtc ctgcgggtcg
     3601 agcatccgca ggatctctgg ccggtgcgcg ccgatgaggg ccagctttcg aacgcggtga
     3661 tgaacctggt cgtcaatgcg cgcgacgcca tgcctcccga aggcggcgcg gtcacgatcc
     3721 gaacctccaa tgtgacgttt gccggggccg agccgatcgg tacgggccat ataccggcag
     3781 gcgattatgt gctgatcgaa gtgatcgata cgggttcggg aatcccgaag gaaaatctca
     3841 ccaagatttt tgaacccttc ttcaccacca agccggtcgg gcaagggacc ggtctcggcc
     3901 tttcgacagt ttatggcgtg gtcaagcaga ccggcggttt catcactgtg gacagcgaac
     3961 gggggcaggg gacatcgttc cgcgtctatc tgccgcgaca taccgccccc agcgcgcccg
     4021 aagtggtcga ggaaagcgaa cgcgccgcgc cgcgagacct cacaggcgaa gacaccattc
     4081 ttttggtgga agacgaagac gcggtgcgga gttttgccgc ccgggctttg cggatgcgtg
     4141 gctataccgt gctggaagca tccggtgggg aggccgcgct cgacatcgtg cgcagccgcg
     4201 cgtccacgat cgacctgctc gtcaccgatg tggtgatgcc caatatggac gggcccacgc
     4261 tggtgcgtgc ggcgcggggc ttgcggccgg atatgcgcgt catcttcatg tcgggctatg
     4321 ccgaggacgc cttccgccgc aatgaggaaa aggtcgagga acttcatttc ctgcccaagc
     4381 cctttggcct gaaacagctc gttgccaagg tgaaagaggt cttgtccgaa gtcccccccg
     4441 cacccaagcc gcctgccccg aacgatagcg gaacatgagt taattttata agtgaatgca
     4501 atggtttatg tcgggacttg tcaaagagaa caaaacatgt acattgcggg agtggttggg
     4561 tccggttgaa ttgctctaag ggccctgggc atttcgtaaa cgttgtgaaa taaggggata
     4621 cggctcatgg cacaggcggt tcttcgcgtg gtggcgaaag aggatagcga caaaaagcgc
     4681 gcgcttgagg cggcgctttc ccagattgat cgggctttcg gcaagggttc ggtcatgaaa
     4741 ctgggccaac gcgagaaggc ggtggacgcg gatgcgattt ccaccggttc gctggggctg
     4801 gatattgcgc tcggtattgg cggattgccc cggggccgcg tggtggaagt ttacgggcct
     4861 gaatcctccg ggaaaaccac gctggcgctg catgtggtgg cggaaattca gaaaaagggt
     4921 ggtgttgcgg cctatgtcga tgccgaacat gcgctcgatc cgggttacgc caagaaattg
     4981 ggcgtggaca tcgacgagct tctgatctcc cagccggata cgggggagca ggcgcttgaa
     5041 atcaccgaca ccctggtgcg ctctggagga gtcgacatcg tggtggtgga ttcggtcgcg
     5101 gctctgacac ccaaggccga gcttgaaggc gaaatgggcg atcaattgcc cggtcttcag
     5161 gcccgcctga tgagccaggc tttgcgcaaa ttgacgggat cgatttccaa atcgaacacc
     5221 attgtcctct tcatcaatca gattcggatg aagatcggga tcatgttcgg gaatcctgaa
     5281 accacgaccg ggggcaacgc gctgaaattt tacgcctctg tccggctcga catccgacgc
     5341 atcggcgcga tcaaggatca tgacgaagtg accggcaatc agacccgcgt gaaagtggtg
     5401 aagaacaagg tggcgccccc gttccgccaa gtcgaattcg acatcatgta cggccatggc
     5461 atttcaaaat ccggggagct ggtcgatctc ggcgccaaag cggggatcgt ggaaaaatcc
     5521 ggctcctggt tttcctatga cggccagcgc atcggccagg gccgggaaaa cgcgaagctc
     5581 tatctggaaa ccaaccagga tgtggccgcc gcgatcgaag cggcgatccg ttccaacgcc
     5641 ggccttttat cggcgggcct gacggttcca aacgacgagc cagctaccgc gggggattga
     5701 accgctccca tcggcatggg acggcttcca gaagggcgct ccttgcgggc gcccttcgcc
     5761 ttttggcgcg gcaaatgcac ccaattgcac cgcaattgca ccattatttt tggcggccgg
     5821 tcacgaaaat ttccatgcga accctggaaa aactcgctgt ttgcgccaca aaacaggggg
     5881 gaggggggtg tcggtgcacg aagcccccac ctcctcgcat gatcggcgcg gtgaagatcg
     5941 ggcggaattt tgcagacgca caaggtgctg ctatttagga tcgcatgaac agtctcaaag
     6001 atattcgcgc cacattcctc gaatttttcc acagctgtga ccatgaggtg gtggcctcat
     6061 cgccgcttgt gccgcgcaat gatccgaccc ttcttttcac caatgccggc atggtgcagt
     6121 tcaagaacgt cttcaccggc caggagaagc gggccgttcc ccgcgccgcg accgcccaga
     6181 aatgcgtgcg cgccggcggc aagcataacg acctcgaccg ggtgggctat accgcgcgcc
     6241 atcacacctt ctttgagatg ttggggaatt tctcctttgg cgattatttc aaggaccgcg
     6301 cgatttcgct cgcctgggac ctcatcacca aggatttcgg gctctccaaa gaccggcttc
     6361 tcgtcaccgt ctatgccgag gacaatgatg ccttcgatct gtggcggaag attgccggtc
     6421 ttcccgaatc ccggatcatc cgcatcggta cgtccgacaa tttctggtcg atgggcgagg
     6481 tcggtccttg cggtccctgt tcggaaatct tctacgacca tggcgaccac attcccggcg
     6541 gtccgccggg atcgccgaat gaggacggcg acaggttcat cgagatctgg aatctcgtct
     6601 tcatgcagta tgagcaattg gccggtggct cccgggaaag cctgccgcgg ccctcgatcg
     6661 acaccggcat gggtttggaa cgcatcacgg ccgtgatgca gggcgtccac gacaattacg
     6721 acatcgatct cttccggcat ctgatcgagg cctcggcgga atattccggc accgcaatcg
     6781 ccggaccgca tgcggtcagc caccgggtga tcgccgatca cttacgctcg tcctcattcc
     6841 tcatggccga tggcgtgctg ccgtccaatg aggggcgcgg ctatgtgctg cgccggatca
     6901 tgcgtcgcgc catgcggcac gcgcatatcc tcggttccaa ggaccctttg atgtaccggc
     6961 tcgtgcccac ccttgtggcg gagatgggcg aagcctatcc ggaactcaaa cgcgccgaag
     7021 cactcttgag cgaaaccttg aagctcgaag aattgcgctt ccgcgacacg ctcgcgcgcg
     7081 ggctgaagct cttggacgag gccacctccg ccatgcagcc gggcgacacg ctgccgggcg
     7141 aaatggcgtt caagctttac gacacctatg gtttcccgcg cgacctgacc gaggacgcgc
     7201 tgcgcgaacg cgggatgaag gtcgatatcg cgggcttcga tttcgccatg gcgcatcaga
     7261 agtccgaggc gcgcaaggcg tggtcgggtt cgggggaagc cgccaccgac gctttgtggt
     7321 tcgcgctgcg cgaggaaatc ggcccaaccg aatttctggg ctatgacacg gaagccgccg
     7381 aaggcgcgat cctcgccatt gtccgagacg gcgaccgcgt gcaatcggga cgcgccggtg
     7441 aaaccctgcg catcatcacc aaccagacac cgttctatgg cgaaagcggc ggacaggcgg
     7501 gcgatcgcgg cgcgatgttc tgcgcgggcg gcgccgaagg cactgtgatc gacaccgaga
     7561 agaagctcgg caatctgtat gtccacgtcg tcgaaatcac ccgtggcgaa ttcaaggtcg
     7621 gcaatgccgt cgatttgcgc gtcgatacgg agcgccgccg cgccacgcgg gccaaccatt
     7681 cggccacgca tcttttgcat gccgcgctca agcatgtgct gggccctcat gtggggcaga
     7741 aaggatcgct cgtctccgcg gaccggttgc gcttcgattt ttcgcatccc aaagcgatga
     7801 cgcccgaaga actcgaacgc gtcgaagacg aggtgaatca ggtcatccgc cagaacaacg
     7861 acgtctcgac ccgtctgatg gcaaccgccc aggccatcga ggccggagcc gtcgccatgt
     7921 tcggcgaaaa atatggcgat gaggtgcgcg tgcttgccat ggggcaggat ttgaacgcag
     7981 cggccaaacc ctattcagtc gaactgtgcg gcggaacgca tgtgcgccgt ctgggcgata
     8041 ttcagctctt cacgatcctg ggcgaatcgg cggtggctgc cggtgtgcgg cgcatcgagg
     8101 cgctcacctc ggagggcgcg cggcgttatc tcgaatcgca aggcgaagcc gcgcgcgaag
     8161 cagccgccgt gctgaagacc tcagccgtgg aattgccctc gcgcatccat gctctcgtcg
     8221 aggatcgccg ccggctggaa cgcgagcttt ccgaagccaa gaa
//
LOCUS       DASYEG010000172         4703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2371459, whole genome shotgun sequence.
ACCESSION   DASYEG010000172 DASYEG010000000
VERSION     DASYEG010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2371459"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2019)
                     /locus_tag="VGM72_07855"
     CDS             complement(<1..2019)
                     /locus_tag="VGM72_07855"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEY4265216.1"
                     /translation="MIAISTRRALLAAAATGVLTATMAATDADARVTRIVIEKKTSPA
                     FNGQAFGSAGQYETLTGKAFGELDPKDPHNAIITDIQLAPKNARGMVEYQATFQIVKP
                     IDMSKASHLMWHDVPNRAGRITIVPAERNFGDIGLSSGWQGDASGATAPKETNDYVVV
                     PVAVNADGSPITGPVIGRITNAKGPDSRTIFVHANPIPYKPASLDTGNATLVTHTSET
                     MDGKIAGEKKVPAGDWAWAKCDAAHPFPGTPDSSQLCLKNGFDPKLLYQVVFTAKDPP
                     VLGIGFAAFRDVGTFFKYDKADDMGTPNPVAGNVTWSISRGVSQSGNFIRALLHLGFN
                     QDEQGRQVYDGAWPIIAGRRIALNVRFAMPDGVMRLYEPGSEGTQSWGPWPDPVRGLP
                     AAGILDRCTASHTCPKIIEHFGAAEMWGLKLSPSFVGTTADKDIPIPDNVRRYYIGST
                     QHGGGPGGFSTTPAAPPACPSNGYGVGSLAANPMPQRETVNAIRVHFRDWVMKNVTPP
                     ASVYPTIAAGTLVEPTKAAMGFPTIPGLPATAPTGFMNPAMDYDYGPDFNYQDGSGIV
                     TKVPPAIKRVIAMKAPKVDADGNEMGGVPVVLREAPLGTYLGWNVTAEGFHKGQLCNY
                     AGGMIPFAKTKAERMTNEDPRPSLEERYHDHAGYVQAVKMAAAKIVAAG"
     gene            complement(2080..3330)
                     /locus_tag="VGM72_07860"
     CDS             complement(2080..3330)
                     /locus_tag="VGM72_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEY4265217.1"
                     /translation="MGLSHGISRKRLLELAALSFMAMVISGPMTPGFAQDANWVTAWG
                     ASQQSLAETKISNASLRMIARVTLPGDGLRIRLDNNFGKAPVIFGHVTVGPRVRGAAL
                     AAGLVKPVTFGGKTSVTVPAGGTVQSDAVAMHVDAEQDIGVSLFVSGADVQPSQHNNA
                     QVTSYLTENGAGDLTDAADGKAFTAKTTRMFWLKSIDVLATGPASSIIAFGDSITDGT
                     CTTLDAHDRWEDVVSQRLALQTPIRRAIVNEGIGGNTVIDVAGYNPPIGSPTGVDRLE
                     RDVLSHSGISHVVLFEGTNDIRRGATADQLIAGMKNIVARVKAKGIKIVGVTIIPRHA
                     VVPGVADTGWNDEKSKIRDRVNAWIRKEAGFDAVLDFDRVIRDPEHPEVMIQAYNCGD
                     GVHPAPIGYFQMGKSVDLGVFEVR"
     gene            3472..>4703
                     /locus_tag="VGM72_07865"
     CDS             3472..>4703
                     /locus_tag="VGM72_07865"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY4265218.1"
                     /translation="MTKPEDGDVYDFVIIGAGSAGCVLANRLSADGRNRVALIEAGGR
                     DNWIWFHIPAGYLFAIGNPRADWMFRTEPDPGLNGRSLAYPRGRVIGGSSAINAMIYM
                     RGQASDYDHWRQMGLTGWGWNDILPLFRKSENHFGGDSEFHGAGGEWHVNEARIRWDL
                     LDAFRSAAHERGIPSKDDFNCGDNEGCGYFHVNQRNGRRLSASSAFLKPALKRPNLTL
                     ITETLVDRIVFDGTRATGVLIRRSDASRPSVIHARREVILSAGAVATPAVLERSGIGD
                     GERLGALGVPVLHHSPGVGENLQDHLQLRLIFKISDVRTMNMDYRSLWKRAGMVLDYA
                     LRRRGALAMAPSQLGAFTRSSPDYVTPNLQFHIQPLSLDKFGDPLHPFAAFTASVANL
                     RPTSRGSIHARSRDCADPP"
BASE COUNT          844 a         1506 c         1449 g          904 t
ORIGIN      
        1 cccggccgcc acgattttcg ccgctgccat cttcaccgcc tgaacatagc ccgcatgatc
       61 gtgatagcgc tcctccagcg agggacgcgg gtcctcattg gtcatgcgct ccgccttggt
      121 cttggcgaac gggatcatgc ccccggcata attgcagagc tgacccttgt ggaagccttc
      181 cgcggtgacg ttccagccca gataggtccc aagcggcgct tcgcgcagca ccaccggcac
      241 gccgcccatc tcgttgccgt cggcatcgac cttcggcgcc ttcatggcga tgacgcgttt
      301 gatggcgggc ggcaccttgg tgacgattcc gctcccgtcc tgataattga aatccgggcc
      361 gtaatcataa tccatggccg gattcatgaa gcccgtcggc gccgtggcgg gaagccccgg
      421 aatcgttgga aaccccatcg cggcttttgt cggctcgacc aatgtgcccg ccgcgattgt
      481 cggataaaca ctggcgggcg gcgtcacatt cttcatcacc caatcgcgga agtgcacgcg
      541 gatcgcgttc accgtctcgc gctgcggcat cggattggca gcgaggcttc ccacgccata
      601 gccattgctg gggcaggccg gcggcgccgc gggcgtggtg gagaacccgc ccggtccgcc
      661 gccatgctgc gtgctgccga tataatagcg gcgcacattg tcgggaatcg ggatgtcctt
      721 gtcggccgta gtgccgacaa aagacggaga gagtttcagg ccccacatct cggcggcgcc
      781 aaaatgctcg atgatcttgg ggcaggtgtg acttgccgtg cagcggtcga ggatgcccgc
      841 ggccggcaac ccgcgcacgg gatcgggcca cggcccccac gactgcgtgc cttccgaacc
      901 cggctcatac agtcgcatga cgccgtcggg catggcgaaa cgcacattca atgcgatgcg
      961 tcgcccggcg atgatcggcc aggcgccgtc atacacctgc cgtccttgtt cgtcctgatt
     1021 gaaacccaga tgcaggagcg cgcggatgaa atttcccgat tgcgatacgc cgcgtgaaat
     1081 cgaccaggtg acattgccgg caacaggatt gggcgtcccc atgtcgtcgg ccttgtcata
     1141 tttgaagaag gtgccgacgt cgcgaaacgc cgcgaaccct attccgagca ccggcggatc
     1201 cttggcggtg aaaaccacct gatacagcag cttgggatcg aagccgtttt tcaggcagag
     1261 ctggctggaa tcgggcgtgc cggggaacgg atgcgcggca tcgcatttcg cccacgccca
     1321 atcgccggcc gggacttttt tctcgccagc gatcttgccg tccatggttt cactcgtatg
     1381 ggtgaccagg gtcgcgttgc cggtgtcgag gctggcgggc ttatagggga tgggattcgc
     1441 atggacaaag atggtccgtg aatccgggcc ctttgcattc gtgatccggc cgatcaccgg
     1501 cccggtgatc ggggagccgt cggcattcac cgcaacgggc accacgacgt aatcattggt
     1561 ctccttgggc gcggtcgcac ccgaggcatc gccctgccag ccgctcgaca gaccgatatc
     1621 gccgaaattg cgctcggcgg gcacgatagt gatgcgcccc gcgcggttcg gcacgtcgtg
     1681 ccacatcaga tggctcgcct tcgacatgtc gatgggcttc acgatctgga aggtcgcctg
     1741 atattccacc atgccgcgcg cattcttggg cgctaactgg atgtcggtga tgatcgcatt
     1801 atgcgggtcc ttgggatcga gctcgccaaa cgctttgccc gtcaacgtct catattggcc
     1861 ggcgctgccg aatgcctggc cgttgaacgc cggcgaagtt ttcttctcga tgacgatacg
     1921 tgtcacgcgc gcgtccgcgt ccgtggcggc catagtcgcg gtcagtacgc cggtggcagc
     1981 ggccgcgaga agcgcacgac gtgtcgatat ggcaatcatg aagacctccc gattttgctt
     2041 gagacagatt atcgcaaagc acgcatcgcg gacatgcgtt tatcgcacct cgaacacccc
     2101 aagatcgacc gattttccca tctggaaata gccgatgggt gcgggatgga ccccatcgcc
     2161 gcaattatag gcctggatca tcacctccgg atgctcggga tcgcgtatca cgcggtcgaa
     2221 atcgagcacc gcatcgaagc ccgcctcctt gcgaatccag gcattcaccc ggtcgcggat
     2281 cttggacttc tcgtcgttcc agccggtgtc ggcgacaccg ggcaccacgg catgacgcgg
     2341 aatgatcgtc acgccgacga tcttgatgcc cttggccttc acccgcgcga cgatgttctt
     2401 catgccggcg atgagttgat cggccgtggc gccccggcgg atgtcgttgg tgccctcgaa
     2461 cagcacgaca tgagagattc cggaatggga aagcacatcg cgctccagcc gatccacgcc
     2521 ggtcggcgaa ccgatgggcg gattgtagcc cgcgacatcg atcaccgtgt tgccgccgat
     2581 gccttcgttg acgatggcac gccggatcgg cgtctgtagc gcgaggcgct gcgacacgac
     2641 gtcttcccag cggtcatggg cgtccagcgt ggtgcaggtg ccgtcggtga tggaatcgcc
     2701 aaatgcgatg atgctgctgg ccggccccgt ggcgagcacg tcgatggatt tgagccagaa
     2761 catcctcgtg gtcttcgccg tgaaggcttt gccgtcggcg gcgtcggtca aatcgcccgc
     2821 gccattctcg gtgagataag aggtcacctg cgcattgttg tgctggctcg gctgcacatc
     2881 ggcgccgctg acgaacaggc taacgccgat atcctgctcc gcatcgacat gcatcgccac
     2941 ggcgtcgctt tgcaccgtgc cgcccgccgg aaccgtcacg ctggttttgc cgccgaacgt
     3001 gacgggcttc accaatcccg ccgcaagcgc tgcgccccga acgcgcggac ccaccgtcac
     3061 atggccgaaa atcacggggg ctttcccgaa attattgtcc agccggatgc gcaatccatc
     3121 gcccggcagc gtgacgcgcg cgatcatgcg caacgacgca ttgctgattt tggtctcggc
     3181 gaggctctgc tgggaggcgc cccacgccgt cacccaattc gcatcctgtg cgaaccccgg
     3241 cgtcatgggg cccgaaatga ccatcgccat aaagctcagc gcggccagtt ccaacagacg
     3301 tttgcgggaa atcccgtgtg aaaggcccat gcctcacccc tcctaccctc ctaccagaag
     3361 gggtgaggct atcgcatcgc tgcaacccga tccaggcgaa ggccccctat gcctggacca
     3421 aacccccggt agagtccggt ctcccagagg tggacaatga aaccggcggc aatgacgaaa
     3481 ccagaagacg gcgacgtgta cgactttgtc attatcggcg ctggctcggc gggctgcgtg
     3541 ctggcgaacc ggctgtccgc cgatgggcgc aatcgcgtcg cgttgatcga agccggcggc
     3601 cgggacaact ggatctggtt ccacatcccc gccggttatc tcttcgccat cggcaatccg
     3661 cgtgccgatt ggatgttcag gaccgagccc gatcccggat tgaacggacg cagccttgcc
     3721 tatccgcggg gccgcgtgat cggcggcagt tcggccatca atgccatgat ctatatgcgc
     3781 gggcaggcat ccgattacga tcactggcgg cagatgggcc ttacgggctg gggctggaac
     3841 gatatcctgc cgctgttccg caagtccgaa aatcatttcg gcggcgatag cgaattccat
     3901 ggcgcgggcg gcgaatggca tgtcaacgaa gcgcgcatcc ggtgggattt gctggatgcc
     3961 ttccggtccg ccgcgcacga aagaggcatt ccgtctaagg acgattttaa ctgcggtgat
     4021 aacgaaggct gcggctattt tcacgtcaat caacgaaatg gacggaggct gagcgcgagc
     4081 agcgcttttc tcaagccggc gctgaagcgg cccaatctca cgctgatcac ggagaccctc
     4141 gtcgaccgga tcgttttcga cggcacgcgc gcaacgggcg tgcttatccg ccgcagtgac
     4201 gcgtcacgcc caagcgtgat ccatgcacgg cgcgaggtaa tcctgtcggc cggcgcggtg
     4261 gcaacgcctg cggttctgga acgctcgggc atcggcgatg gggagcgcct gggcgcgctg
     4321 ggtgtgcccg tcctgcacca ttctcccggc gtcggcgaaa atctgcagga tcatctgcag
     4381 ctccggctga tcttcaagat cagcgatgtc cgcaccatga atatggatta ccgttcacta
     4441 tggaaacgcg ctgggatggt gctggactat gcgttgcgcc ggcgcggggc ccttgccatg
     4501 gcgccctcgc aattgggggc cttcacgcgc tcgtcgcccg actatgtgac gccaaacctg
     4561 caattccata tccaaccgct gtcgctcgac aaattcggcg acccgctgca tccgtttgca
     4621 gccttcaccg ccagcgtcgc gaatttgcgg ccgaccagcc gcggctccat ccatgcgcgg
     4681 agccgcgatt gtgccgatcc gcc
//
LOCUS       DASYEG010000173         6505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4404859, whole genome shotgun sequence.
ACCESSION   DASYEG010000173 DASYEG010000000
VERSION     DASYEG010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4404859"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VGM72_07870"
     CDS             <1..280
                     /locus_tag="VGM72_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009364566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HEY4265219.1"
                     /translation="NEIQTSQKPAVAVKRSVNPDYIICLEDGKKLKMLKRYLRSNYNL
                     TPEEYRAKWGLPADYPMVAPNYAAQRSEFAKKIGLGRTASSSGRRRKG"
     gene            295..693
                     /gene="msrB"
                     /locus_tag="VGM72_07875"
     CDS             295..693
                     /gene="msrB"
                     /locus_tag="VGM72_07875"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY4265220.1"
                     /translation="MTDKIAKPEEEWRKQLTPLQYAVTREKATERPHTGEYDKTTTPG
                     TYRCICCGQELFQSDDKFDAHCGWPSFTRPSDEASVAAEADNSHGMRRTEVLCSKCDA
                     HLGHVFDDGPGPTGQRYCINSASLKLDAKK"
     gene            complement(696..902)
                     /locus_tag="VGM72_07880"
     CDS             complement(696..902)
                     /locus_tag="VGM72_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265221.1"
                     /translation="MPAVRSGAGREVLIEIVTLGTFSKVTAIDSATGTEVSVTGPANG
                     SRASLEAAALSKLKFVLKKKTDGP"
     gene            complement(902..2119)
                     /gene="hemA"
                     /locus_tag="VGM72_07885"
     CDS             complement(902..2119)
                     /gene="hemA"
                     /locus_tag="VGM72_07885"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HEY4265222.1"
                     /translation="MPFAYGHIFKDNLAKLKREGRYRVFADILRHQGQFPAAVHHTAN
                     GTRSITVWCSNDYLCMGQNAKVLAAMKEAVDKAGAGSGGTRNISGTNHFHVELERELA
                     DLHGKESALLFTSGFVSNDATLSTLARLLPGCIFFSDELNHASMIEGMRHSGAEKRIY
                     RHNDMAHLDEILKAAPPGAPKIICFESVYSMEGDFGQIGKICDLAEQYGAMTYLDEVH
                     GVGLYGPRGGGVAERDGVMHRVDIIEGTLAKAFGVMGGYITGTTAIMDCVRSFASGFI
                     FTTSLPPAITGGALAAVRHLKESNVEREGLQERAARLKGLLGKRNVPLIDSPSHIVPV
                     LVGDASLCKVVSDELLFTHGVYVQPINFPTVPRGTERLRITPSPLHTDAQMDHLVEAI
                     DTVWTALRLRRAA"
     gene            complement(2269..2727)
                     /locus_tag="VGM72_07890"
     CDS             complement(2269..2727)
                     /locus_tag="VGM72_07890"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HEY4265223.1"
                     /translation="MLRPRYLECLNLVERLHRQLLDVIKDELDRRGERVVNSVQALLL
                     FNIGDQELTAGELRTRGHYLGSNVSYNLKKLVESGYIHHERSDVDRRSVHVSLTDKGA
                     EIRETVAKLFSRHLESIEAVGNVAANNFEDLNTSLRRLERFWLDQVRYRL"
     gene            complement(2874..3923)
                     /locus_tag="VGM72_07895"
     CDS             complement(2874..3923)
                     /locus_tag="VGM72_07895"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF004127.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY4265224.1"
                     /translation="MAYIGGMDGNDIFIRRDGGLGRITLNRPQSLNALTHQMCASMLE
                     ALRAWTEDPAIRACVIDAVPGRAFCAGGDIRAIYDMGKRRDGSAQAFFATEYRLNMAV
                     KHFPKPYIALIGGIAMGGGVGISIHGSHRVVSENALFAMPETAIGLFPDVGGTYALPR
                     LPGEIGMYLALTGARLGAADMVCANIATHFVPAVKFGEIVQRLVAGEAPDAVLAGLAA
                     DPGTPPLATTRAAIDRAFVMPSVEAVMDALAREEEWGNETAAILATRSPTSLKLTFKA
                     MREGKRLDFDSCMRMEYRLTMRALERPDFYEGVRAAVIDKDQRPRWQPPALAGVSDAE
                     IACYFAPLDVTELSS"
     gene            4011..5012
                     /gene="hemB"
                     /locus_tag="VGM72_07900"
     CDS             4011..5012
                     /gene="hemB"
                     /locus_tag="VGM72_07900"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005179750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEY4265225.1"
                     /translation="MPHLPPAYPNLRMRRLRRHDWARRLVAENSLSPADLIWPLFLID
                     GENKRVPIASMPGVERLSVDAAVGAAEEASGLGIPAIALFPNTEDSKKTADGREAENP
                     DNLVCRAVRAIRKAVPDIGIMCDVALDPYTSHGHDGVLIDGYVANDATLQVLVRQAQV
                     QAEAGCDILAPSDMMDGRIGAIRTMLEAKGHHDIILLAYAAKYASAFYGPFRDAVGSA
                     KALTGDKRTYQMDPANTDEALREVALDIAEGADMVMVKPGMPYLDVLRRVKDEFGMPT
                     IVYQVSGEYSMLMAAIERGWLEHERAIEESLTAFKRAGADGIITYFARDIARRLKGR"
     gene            complement(6020..>6505)
                     /locus_tag="VGM72_07905"
     CDS             complement(6020..>6505)
                     /locus_tag="VGM72_07905"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WHG domain-containing protein"
                     /protein_id="HEY4265226.1"
                     /translation="LSLRSVARRAGVSHAAPYRHYASREALLADIAVEGLGRLRAELA
                     QAATREGDRSDRIVRIGGAYLRFASRHEGLLRLMFGSELPNRRDHPGLAEATALIGED
                     IGAALGDAAAGLAVWAAVHGLALLILENVIDLGQKEAGLAVAPSRAEIILRSLVEAMR
                     D"
BASE COUNT         1337 a         1969 c         1949 g         1250 t
ORIGIN      
        1 aaatgaaatc cagacaagcc agaaacctgc ggtggccgtc aaacgttccg tcaatcccga
       61 ctacatcatc tgtctggaag acggaaagaa attgaaaatg ctgaaacgct atttgcgctc
      121 caactacaat ttgacgccgg aagaataccg cgcgaaatgg ggcctgccgg cggattatcc
      181 gatggtcgcg cccaattatg ccgcacaacg gtcggaattc gccaagaaga tcggcttggg
      241 gcgcacggca tcctcgtcgg gccggcggcg caaaggataa agcaggagcc gcgaatgacg
      301 gacaagatcg caaaacccga agaggaatgg cgcaagcaac tcacaccgct gcaatatgcg
      361 gtaacacggg agaaggcgac ggaacgcccc cataccggcg aatatgacaa gacgaccacg
      421 cccggcacgt atcgctgcat ctgctgcggc caggagctgt ttcagtcgga cgacaaattc
      481 gatgcccatt gcggctggcc cagcttcacg aggccgagcg atgaggcaag cgtcgccgcc
      541 gaggccgata acagccacgg catgcgacgc accgaagtcc tgtgctccaa atgcgacgcg
      601 catctcggtc acgtcttcga cgacggcccg gggccgaccg gccagcgcta ttgcatcaat
      661 tccgcatcgc tgaagctgga cgcgaagaaa taagctcagg gaccatcagt tttcttcttc
      721 agcacgaatt tcagtttgct aagggccgcg gcctccaggc tcgcgcggga gccattggcg
      781 gggccggtga cggaaacctc cgtccctgtg gcggaatcga tcgccgtcac cttggagaag
      841 gttccaagcg tcacgatctc gatgagaacc tcgcgccccg ccccggaacg gaccgccggc
      901 atcaggccgc gcggcgcagc ctcaacgccg tccagacggt gtcgatcgct tcgaccagat
      961 gatccatctg agcgtcggtg tggagcggcg atggcgtgat gcgcaggcgt tcggtgccgc
     1021 gcggcaccgt ggggaaattg atcggctgca catagacgcc atgggtgaag agcagctcgt
     1081 cggagaccac tttgcacagc gaggcgtcac ccacgagcac cggcacgatg tggctcggcg
     1141 aatcgatcag cggcacgttt cgttttccga gcagaccctt caggcgtgcg gcgcgctcct
     1201 gcaaaccttc gcgctccaca ttgctttctt tcaggtgccg cacggcggcc agcgcgcccc
     1261 ccgtgatcgc gggcggcagc gacgttgtga agatgaaacc ggaggcaaag gaccgcacgc
     1321 aatccatgat cgccgttgtt ccggtgatgt agccgcccat cacgccgaaa gccttggcga
     1381 gtgtgccttc gataatatcg acgcgatgca tcacgccgtc gcgctcggcg acgccgccgc
     1441 cgcgcggacc gtagagcccg acgccatgca cctcgtccag ataggtcatg gcgccatatt
     1501 gttcggcgag atcgcaaatc ttcccgatct gaccgaaatc gccttccatc gagtagaccg
     1561 attcaaagca gatgatcttc ggcgcgcccg gaggcgcagc cttcaagatc tcatccagat
     1621 gcgccatgtc gttatggcga tagatgcgct tttccgcccc cgaatgacgc atgccctcga
     1681 tcatcgaggc atggttcagt tcatccgaga agaaaataca gccgggaagc agacgtgcca
     1741 gcgttgacag cgtcgcatcg ttcgagacga aacccgaggt gaagaggagc gccgattcct
     1801 tgccgtgcag atcggcaagt tccctttcca gttcgacatg gaaatgattg gtgccggaaa
     1861 tgttccgtgt gccgccggac cctgcgccgg ccttgtcgac cgcctctttc atggcggcaa
     1921 gaaccttcgc gttctggccc atgcagagat agtcgttgct gcaccatacc gtaatcgacc
     1981 gtgtcccatt ggccgtatga tggacggcgg cgggaaattg accctggtgg cgcaggatat
     2041 cggcaaagac ccgataccgg ccttcgcgct tcagcttcgc cagattgtcc ttgaagatat
     2101 ggccgtacgc gaacggcatg tgatccctgc tctccccggc ccgaccgatt ttctatagga
     2161 cgcctgtccg ggaagatagc ccaagagagt tccttcgcgc atgtgaattg tcacagcgtt
     2221 aaggctgtat tcgcaaaaac ttgcgatgta cttgagaatg agtcgcgact agagccggta
     2281 gcggacctga tcgagccaga atctttcaag ccggcgaaga ctcgtgttga gatcctcgaa
     2341 attgttcgcg gccacattgc cgacagcctc aatcgattcg agatgccggc tgaacagttt
     2401 ggcgacggtc tcgcgaatct ccgcgccctt gtccgtcaga ctgacatgga ccgagcggcg
     2461 gtccacgtcg gaccgctcgt gatggatata gccggattcg accagtttct tcagattgta
     2521 cgagacgttg gacccaagat agtgcccgcg cgtgcgcaat tcgcccgcgg tcaattcctg
     2581 atcgccgata ttgaaaagca gcaacgcctg gacgctgttc accacgcgct cgccgcggcg
     2641 gtcaagttcg tccttgataa cgtcgagaag ctgacggtgg agacgctcca cgagattcag
     2701 acattcgaga taacgtggcc ggagcatttg cgactcatcc aactgctcgg ccttttccat
     2761 tttcgcgacg acggtcatac cttccccttg gcgtcttgcc agcggaaaaa ttaccagagg
     2821 agcgttaatg gcggattagc gcggcacgaa tgctttgtgc caatttgaag cgcctaggac
     2881 gataattctg tgacatcgag gggcgcgaaa taacacgcga tttcagcgtc tgaaacgcct
     2941 gcaagtgccg gcggctgcca gcgcggacgc tggtccttgt cgatcacggc cgcacggacg
     3001 ccctcgtaaa aatcgggcct ttcgagcgcg cgcatcgtca ggcgatactc catgcgcatg
     3061 caggaatcga aatccaggcg ctttccctcg cgcatggcct tgaatgtgag cttgagactc
     3121 gtaggcgaac gggtggcgag aatcgcagcc gtttcgtttc cccattcctc ctcccgcgcg
     3181 agtgcgtcca tgacggcttc gaccgatggc atgacgaagg ccctgtcgat ggccgcgcgc
     3241 gtcgtggcca aggggggcgt accgggatcg gccgccagac ccgccaacac cgcatcgggg
     3301 gcttcgcccg cgacgaggcg ttgcacaatt tcgccgaatt ttaccgccgg cacgaaatgt
     3361 gtcgcgatgt tcgcgcaaac catgtcggcg gctccgagcc gtgcgccggt cagtgcgaga
     3421 tacattccga tctcgccggg cagacgcggc agcgcatagg tgccgccgac atcgggaaag
     3481 agaccgatgg ccgtttcggg catggcgaag agtgcattct ccgacaccac gcggtgggaa
     3541 ccatgaatgg aaattccaac gccgccgccc atcgcgatgc cgccgatgag cgcgatatag
     3601 ggtttcggga aatgcttcac cgccatgttc aggcggtatt cggtggcgaa gaacgcttgc
     3661 gcgctgccat cgcgcctttt gcccatatca tagatggcgc gaatatcgcc gcccgcgcag
     3721 aacgcgcgtc ccggcaccgc atcgatcacg caggcgcgaa tggccgggtc ttcagtccag
     3781 gcgcgaagcg cctccagcat ggacgcacac atctgatggg tcagcgcgtt cagcgattga
     3841 ggccggttga gcgtgatgcg tccgagaccg ccatcccggc ggatgaagat gtcattgccg
     3901 tccatgccgc caatgtatgc caaagcgcca ttgtacgcga aagcgggaca ttgaaggctg
     3961 tgtctgaggc actatagtgc ggcttctctg tcgcaatcag gtttaagccg atgccgcatc
     4021 ttccgcccgc ctacccaaac ctgcgcatgc gccgcttacg ccggcacgat tgggcccgcc
     4081 gcctggtggc ggagaattcg ctgtcgccag ccgatctgat ctggccgctg ttcctcatcg
     4141 acggtgaaaa caagcgcgtt cccatcgcct ccatgcccgg cgtggagcgc ctctctgtcg
     4201 atgcggcggt gggagccgcg gaagaagcga gtggactcgg catccccgcg atcgcgctct
     4261 ttcccaacac cgaggattcc aagaagaccg ccgatgggcg cgaagcggag aacccggaca
     4321 atctggtctg ccgcgccgtg cgcgcgatca ggaaggccgt gcccgatatc ggcatcatgt
     4381 gcgatgtggc gctcgatccc tataccagcc acggccatga cggcgttctg atcgacggct
     4441 atgtcgccaa tgacgcgacg cttcaggtgc ttgtgcgcca ggcccaggtc caggccgaag
     4501 ccggctgcga tattctggcg ccgtccgaca tgatggacgg acgcatcggc gccatccgca
     4561 ccatgctgga agcgaagggg catcacgaca tcatcctgct cgcctatgcg gcgaaatatg
     4621 cgtccgcctt ctacgggccg ttccgcgatg cggtggggtc ggccaaggcg ctgaccggcg
     4681 acaagcgcac ctatcagatg gatccggcga acaccgatga agccttgcgc gaagtggcgc
     4741 tcgacatcgc ggagggggcc gacatggtga tggtaaagcc gggcatgccc tatctcgacg
     4801 tgttgcggcg cgtgaaagat gagttcggca tgccgaccat cgtctatcag gtgtccggcg
     4861 aatattcgat gctgatggcg gccatcgaac gcggctggct tgagcacgaa cgcgccatcg
     4921 aagaaagcct caccgccttc aagcgcgcgg gcgccgatgg gatcatcacc tatttcgccc
     4981 gcgacatcgc ccgccggcta aagggccgct aagccaagcc tcaaaacaac tcggatacgt
     5041 cgtgtatgtc tgctttggag agaacccgac acttgggtct gtctcagaaa tgtcggcttc
     5101 tgacccaaag cagacccaaa atgcccgtca tggtggggtt atttctgcta ttctaaaagg
     5161 tgcaaggatt ttggaataca gtaccaaatg atgtccggcg ctgaggctat ttatagggct
     5221 tgggttattc tcggcattgg cgctttcttc ctcttgttgg ccgctctcca attatggttg
     5281 ggcaaggcgt ttctcggtta tggatggagt tggcatccgt caccgtggtt ctttcggaag
     5341 aaagaacctt tccaattttg gtcgcatgtt ggtctgttgg cctggttggg cctatttaca
     5401 attggcttgg cggcgacaag gcttctctag gcgcaatcct caagcgtccg tttctggcgc
     5461 gagaattgga cattcataga ttggccgcca aaaatcgccc tggaccaagc gactccaccg
     5521 gaggcaggta aatccttgcg ttacctcagg atagaatgtg gcccatggcc cgagggaaga
     5581 cacccatggt gggtcaatgt actccggcgg gtcctacctg ctgccaatcc tgatcttgag
     5641 ccactgtatg cgaaggccaa attgtggtgg ctcgagatca gtgacgacgg gcagcctctt
     5701 cgcgaaatcg gatttgatgc tacaggcagt gccgttgttc tcggaccaat cggccgaaac
     5761 tgcggcctgc tggtagatag cgcggacgat tggagcgatt ccgacctcga ttcagccgaa
     5821 gcggcatcgc acttcgacga gaaatggtta tcccttcttc cgagttttcg acacttggat
     5881 tcgagataac ctccgattcg aagcctgact attccgctcc actcgaatgt ccgcaactgg
     5941 ggcgaatagc cgacatgcgt agcccgctca aaatgtcggt ttctggaccc aaagcagacg
     6001 tgatttgatc tgcgggcgtt caatcgcgca tggcttccac gaggctgcgc aggatgatct
     6061 cggcgcgcga cggggccacc gccaaccccg cttccttttg gccgagatcg atgacgttct
     6121 ccaggatcag cagcgcgagt ccgtgcaccg ccgcccatac ggcaagtccg gctgccgcat
     6181 cgccaagtgc ggcgccgatg tcttcgccga tcagagcggt tgcctccgcg aggccaggat
     6241 ggtcgcggcg attggggagc tccgacccga acatcagccg cagcaaacct tcgtggcgcg
     6301 aagcaaagcg caaataggcg ccgccgatac gcacgatgcg atcggaccga tcgccttccc
     6361 gggtggcggc ttgcgccaac tcggcacgca aacgtcccag cccctccacc gcgatgtcgg
     6421 caagcagcgc ctcgcggctc gcataatggc gatagggtgc tgcatgggaa acgcccgcgc
     6481 ggcgcgcaac cgaacgcagc gacag
//
LOCUS       DASYEG010000174         9912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2953539, whole genome shotgun sequence.
ACCESSION   DASYEG010000174 DASYEG010000000
VERSION     DASYEG010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 9912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9912
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2953539"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1658)
                     /gene="phaC"
                     /locus_tag="VGM72_07910"
     CDS             complement(<1..1658)
                     /gene="phaC"
                     /locus_tag="VGM72_07910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEY4265227.1"
                     /translation="MAKSPRTKKSVEEKAEAQPRESEPRQNLYQLNDPSAFGRNMARV
                     AAQSQQLLSEFLKRQTERFGQQPLDPLNLAGAYFSLLKHMTADPSHMFTAQIALWRDY
                     LNLIQRTGERALGRPVDPFVVPASGDRRFRDRDWQENQIFDFIKQSYLVTSNWLQKTV
                     AGIQGMDAREQARASFYAKQFVDAIAPSNFVLTNPEVLRETLRSNGDNLVKGLDNLLS
                     DLDRGQGELSIRQTTDNFVVGRDVATTPGKLIFRNELFELLQYSPTTETAYEIPLLIF
                     PPWINKFYILDLQPKNSFVKWAVERGYTVFVVSWVNPDPSLAERDFEDYMREGVFAAL
                     DAVQKATGQRQVNAIGYCIAGTLLAATLAYIAGSGENQDRIRSVTFLAAQVDFSEAGD
                     LLVFVDDEQLAAMEQQMRAAGGVLEGSKMALTFNMLRANDLIWSFVVNNYLLGKEPVP
                     FDLLYWNSDTTRMPLKLHLAYLRQCYRDNALAKAQMILGGERLNLAKIMTPAFFQAAK
                     EDHIAPAASVYKGAKLFGGPVTYMLAGSGHIAGVINAPAAGKYQ"
     gene            1877..2176
                     /locus_tag="VGM72_07915"
     CDS             1877..2176
                     /locus_tag="VGM72_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265228.1"
                     /translation="MGLRAFALPGVCALAGFLLTGCSTLPEIGGAPLPPEPGNAHPVY
                     RDLADIPEVPAVTPAENNENTIRVLAEDRIKAAQAAQNLRREPFDQPDSAMPSGF"
     gene            2179..3393
                     /locus_tag="VGM72_07920"
     CDS             2179..3393
                     /locus_tag="VGM72_07920"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: protein motif:HMM:NF006604.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEY4265229.1"
                     /translation="MQTEFHRISRLPPYVFEQVNKLKAEARARGEDIIDFGMGNPDMP
                     TPAHIVEKLIETARDPRSNRYSASRGIKGLRRAMAAYYQRRFGVELNPDSEVIATLGS
                     KEGFANIAQAITAPGDVVLVPNPAYPIHAFGFILAGAAIRHVPAPSPEEFLSRAADAA
                     HQSQPTPLAIVVNYPSNPTAQMASLDFYKEVVALAKRHSIWVLSDCAYADTYFDDSNP
                     PPSILQVEGAKDIAIEFQSLSKTYAMPGWRMGFAAGNRTLIAALGRIKSYLDYGAYTP
                     IQVAATAALNGPQDCVDEIRAIYRSRRDVLVESMARAGWEIPSPAATMFAWAPIPESF
                     RHAGSMEFAKQLLVEAKVAVSPGIGFGEYGEGYVRIALVENEQRIRQAARNVKRFLSD
                     VGRNSKAMEVAK"
     gene            3390..4679
                     /locus_tag="VGM72_07925"
     CDS             3390..4679
                     /locus_tag="VGM72_07925"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEY4265230.1"
                     /translation="MSGALRVGLAGLGTVGIGVAKVLLGRNALAWRAGREIAIAAVSA
                     RDKTKDRGIDLSTIAFENDPLALAERADVEVVVELIGGEEGVARTLVESALRARKHVV
                     TANKALLARHGASLAALAERNGVSLKFEAAICGGIPIVKALREGLIAYEVTAVRGILN
                     GTCNFILTQMEATGRPFAEVLAEAQRLGYAEADPTLDIGGGDTAHKLTLLASLAFGTV
                     PDLDGVAMTGIDKVSPADIAFAGEFGYRLKLLGVARRTVNGIEQLVHPAMVPARTPLA
                     DVESSFNAVSADAGDAGTFFFQGRGAGRGPTTSAVVSDLVDVARGAFGPVFGRPAAEL
                     AQPRREPAEARMSAYYLRFQVLDVPGVMAEISRHLANERVSIESMIQRGRAPGEPVAI
                     VMITHEAQHVAVMRALKAIAASDKVFEMPCMIPMETA"
     gene            4702..5664
                     /gene="glpX"
                     /locus_tag="VGM72_07930"
     CDS             4702..5664
                     /gene="glpX"
                     /locus_tag="VGM72_07930"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEY4265231.1"
                     /translation="MSRPLDRILALEAVRVTEAAAIRAFKVAGRGDEHAADQAAVEGM
                     RWAFNLLPIDGTVVIGEGERDEAPMLFIGEKVGSGGLKVDIALDPLEGTALCAKGLPN
                     AIAVMAMAEAGTLLNAPDVYMDKIAIGPGFPEGTIDLDADPADNIIALAKAKNVKPER
                     IRALVLDRQRHEKIIASIREAGASVQLITDGDVAGVIATTEPETTGIDIYMGQGGAPE
                     GVLAASALRCVGGQLQTRLVFRNDAERQRAERWGIKDLNRKYRLTDLASGDVVFSATG
                     VTDGSMLEGVHVHATYVTTHSIVMRSATKTVRWIKMRHPLDDAG"
     gene            5654..7492
                     /gene="recJ"
                     /locus_tag="VGM72_07935"
     CDS             5654..7492
                     /gene="recJ"
                     /locus_tag="VGM72_07935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEY4265232.1"
                     /translation="MLAEASPRIFQHTDAAPQETASGFVLDVASSFLGRAWRMRPYDL
                     ARMRELEWSGLNPALAQILASRGVHRDVAESFLDPRLKTSLPDPYCFANMERAARRFA
                     AAVIQGEIIAILGDYDVDGACAAALLLRFLRGMNREALLYVPDRMTEGYGPSVGAVAS
                     LRAQGANLLVTVDCGAAAHEALQAARDCGLDAIVLDHHAVDVNPPAFAHVNPNGPDDS
                     CGITYVCAAGLVFIFLVAVQRILREEGWFASQGLDETNLLDQLDIVALATIADVVPLK
                     GINRAFVRQGLRRLERLERPGLAALARLASAEPPFTAFHLGFVFGPRINAGGRVGRCD
                     LGARLLASDDPAEACALGLELDRHNRERRAIEALILESAEASAQAQSHSPFLLVSGDG
                     WHPGVVGIVAGRLKERHAKPVLVAGFEAGGETAIGRGSARSVAGVDLGAIIRTARAEG
                     LLETGGGHAMAAGFSLKREQLPAFGDFLNQQIEPQRSMITLASEMTGDALLSPSGATL
                     GLLDEMDRAGPYGAGNPEPLFFMPDMLVVYAGLAGTNHVRVRLVGRDGQGIGAIAFRA
                     GGSPLGEGLLKARGARVHVTGRLKRDDYDGVAKVQLHIEDAAPAEP"
     gene            7551..7625
                     /locus_tag="VGM72_07940"
     tRNA            7551..7625
                     /locus_tag="VGM72_07940"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7584..7586,aa:Glu,seq:ttc)
     gene            7712..9451
                     /locus_tag="VGM72_07945"
     CDS             7712..9451
                     /locus_tag="VGM72_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY4265233.1"
                     /translation="MARILMGVVRKKQTGMYTARKRVPPRLQAAVGKLSDPPRDRVAW
                     LQKSLGTKDLSRANLLGKRVLMDFDKIIARAEASLAPPPMQADLSDREIEQIASYFFA
                     EVLEEDEEARTHGLSDEKRPGIDPEFGLSARQMEEFTESRDGVLAASQQQLARGDTST
                     IQSEVERVLEDFGISLDRKSVAFRKLSMAMLREFVRAMKALQDRDKGEPIPTPKARMP
                     PVQAAVAGSGETLTAALKGWEKARAPSPNTLQEFQYGIGRFVEFHGDMPVVKITRKHV
                     AQYREALQDIPKRRSGKLLKASLPELVEWTKQHPTAQRLSAGTVNKLLGAVQTIGIWA
                     RDNGVIPDDVQWSDPFSNMRLDEKEPERAPWDVPELNTLFASPVFTAHKRPQAGGGEA
                     AFWLPLLGLFTGARQGELGPLRTGDIATDATTDIVSISITEDATKNKRLKTRSSRRTV
                     PVHPELARLGFLRFVDARREKDGQDAPLFPLLKPGPKGSVAESWSKWFGRYIRSIGIN
                     DSSRVFHSFRHSFKDGLRAAGVGEDINDALTGHSNASVGRSYGAKDSVRRFGLNRLAA
                     AVAGVTYEGVKLP"
     gene            complement(9563..9802)
                     /locus_tag="VGM72_07950"
     CDS             complement(9563..9802)
                     /locus_tag="VGM72_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265234.1"
                     /translation="MGVAVGSDLTGKGKDESKVPMVLVVNAAIASIAGSLAAHDLMGF
                     VGAETSPALLGLLAGLLSAVLMAMLMITYHTHPER"
BASE COUNT         1896 a         2851 c         3211 g         1954 t
ORIGIN      
        1 tgctgatatt ttccggccgc gggcgcattg atgacaccgg cgatgtgacc ggaacccgcg
       61 agcatgtagg tcacgggtcc gccaaacagt ttcgcgcctt tgtagacgga cgcggccggt
      121 gcgatgtggt cttccttcgc cgcctggaag aaggcgggcg tcataatttt cgcaagattc
      181 agccgttcgc ctccgaggat catctgggct ttggccaggg cattgtcacg atagcattgg
      241 cgcaaatagg caagatgaag cttcagcggc atccgtgtgg tatcggaatt ccaatagagc
      301 aggtcgaacg gaacaggctc tttgccaaga agataattgt taaccacgaa cgaccagatc
      361 agatcgttcg cgcgcaacat gttgaaggtt aacgccatct tggaaccttc gagcacgcca
      421 ccggccgcgc gcatttgctg ttccatggcg gccaattgtt cgtcgtccac gaagaccaac
      481 agatcgccag cttccgaaaa atcaacctgg gcagccagga atgtgaccga tcttatgcga
      541 tcctgatttt cgccgcttcc cgcaatatag gcgagcgtgg ccgcgagcag cgtacccgcg
      601 atgcaataac cgatcgcatt gacctggcgc tggcctgtgg ccttttgcac ggcatccagc
      661 gcggcgaaga cgccttcgcg catgtagtcc tcgaaatccc tttcggcgag cgacggatcg
      721 ggattgaccc aggagacaac gaaaacggta tagccgcgct ccaccgccca tttgacgaag
      781 gaattcttgg gctgcagatc gaggatgtag aatttgttga tccagggcgg aaatatcagc
      841 agcgggattt cgtaggctgt ctccgtcgtg ggcgaatatt gcagcagctc gaacaattca
      901 ttgcggaaaa tcagtttgcc cggtgtggtc gcgacatcgc ggccgacgac aaaattgtcg
      961 gtggtctggc ggatggacag ttcgccctga ccgcgatcga gatcggagag aagattatcg
     1021 agtcctttca ccagattatc gccattgctc cggagcgtct cgcgcagcac ttcgggattg
     1081 gtgagcacaa aattggacgg cgcgatcgca tcgacgaatt gcttggcata gaaggatgcg
     1141 cgcgcttgtt cgcgtgcgtc catgccttgg attccggcca cggttttctg cagccagttg
     1201 gatgtcacga gataggactg tttgataaaa tcgaaaatct gattttcttg ccaatcccga
     1261 tcgcggaaac ggcgatcgcc cgatgccggc acgacgaaag gatccacggg acggccgagc
     1321 gcgcgctcgc cggtgcgctg aatcagattg agataatctc gccaaagagc gatctgagcg
     1381 gtgaacatgt ggctcggatc ggccgtcata tgcttcagca gggaaaaata tgcgcccgcc
     1441 agattcagag gatcgagggg ctgctggccg aagcgttcgg tttggcgctt cagaaattcg
     1501 gataggagtt gctggctctg ggccgccacc cgcgccatgt tgcggccgaa ggcggagggg
     1561 tcgttaagct gatagaggtt ttgccgcggc tcgctctcgc gtggctgagc ctccgccttt
     1621 tcttcgacgg atttcttggt acggggtgat tttgccatga ggacgtccga aataggggct
     1681 aagagtcgaa atgctgcata tgcgaaaata tggggctgtg gagccctgaa cggaagggca
     1741 aatatccgga aaaaacgcgt gagccgtgtt ggaacacgat atcatggccg gtatatggtg
     1801 ttttgccgct gtgcggaatt tgcggcgatt tgaaaggttt gacgtgaagc ctggcgcggg
     1861 aaaagatcga tcgaaggtgg ggctgcgcgc ttttgccctg cctggggtct gtgcactggc
     1921 cggctttttg ctgaccggat gtagcacgct gcccgagatc ggcggcgcgc ctttaccgcc
     1981 ggaaccgggc aacgcgcacc cggtttatcg cgatcttgcg gatattccgg aagtcccggc
     2041 ggtgaccccg gcggaaaaca acgagaatac gatccgcgtc ctggcggaag atcgcatcaa
     2101 ggcggcgcag gccgctcaga acttgcgccg ggaacctttc gatcagcccg attctgccat
     2161 gccgtcaggg ttttagtcat gcaaaccgaa tttcaccgta tcagccggct tccgccctat
     2221 gtcttcgagc aggtcaacaa gctcaaggcc gaggcgcgtg cccgcggcga agacatcatc
     2281 gatttcggca tgggcaatcc ggacatgccg acgccggccc atatcgtgga gaagctgatc
     2341 gagaccgcgc gcgatccgcg atccaatcgt tactcggctt cgcgggggat caagggcctg
     2401 cgtcgcgcga tggccgcgta ttatcaacgc cgttttggag tcgaattgaa ccccgatagc
     2461 gaagtgattg cgacgctcgg ctccaaggag ggctttgcca atatcgcgca ggcgatcacg
     2521 gcgccaggcg atgtggtgct ggtgccgaac ccggcctatc cgatccatgc gttcgggttc
     2581 atccttgccg gcgcggcgat ccgccacgtg ccggctccct cgccggagga atttctatcg
     2641 cgcgcggccg atgcggccca tcaatcgcag cccacgccgc tcgccattgt cgtgaactat
     2701 ccgtcaaatc ccacggcgca gatggcgagt ctcgatttct acaaggaagt cgtagccctc
     2761 gcaaaacggc acagcatctg ggttttgtcg gattgtgcct atgccgacac ctatttcgac
     2821 gacagcaatc ctccaccctc gatccttcag gtggaggggg ccaaggatat cgccatcgaa
     2881 ttccagtcgc tttccaaaac ctatgccatg ccgggatggc gcatgggatt tgcggcgggc
     2941 aatcgcacgc tgatcgcggc actcggccgg atcaaatcct atctcgatta cggagcctat
     3001 acgccgatcc aggtcgcggc gactgcggcg ctgaacggtc cccaggattg cgtggacgag
     3061 attcgcgcca tctaccgttc gcgccgggac gtgctcgtgg aaagcatggc ccgcgccgga
     3121 tgggaaattc cgagccccgc ggccaccatg ttcgcctggg cgccgattcc ggaatcgttc
     3181 cggcatgccg gttcgatgga attcgccaag caattgctgg tggaagcgaa agtcgccgtc
     3241 tcgcccggga tcggatttgg cgaatatggc gagggttatg tgcggatcgc cttggttgag
     3301 aatgaacaac gcatccggca agccgcgcgt aatgtgaaac gattcctgag cgatgtgggc
     3361 cgcaattcca aagccatgga agtggccaag tgagcggggc cctacgggta ggtttggcgg
     3421 gtctcggcac tgtcgggatc ggagtggcga aggtcctttt ggggcgcaac gcgcttgcct
     3481 ggcgcgccgg ccgtgaaatc gccatcgccg cggtctcggc gcgcgacaaa acgaaggacc
     3541 gtggaatcga tctgtccaca atagcgttcg agaacgatcc tctcgcgctc gccgaacgcg
     3601 ccgatgtcga ggttgtcgtc gagttgatcg gtggcgaaga aggggtggcg cgcaccctgg
     3661 tcgaatcggc cctgcgtgcg cggaagcatg ttgtgaccgc caacaaggcg cttctggcgc
     3721 ggcacggcgc aagcctcgcg gctctggccg agcgaaacgg cgtttcgctg aagttcgaag
     3781 cggccatctg cggcgggatc cccatcgtca aggcgttgcg ggaggggctg atcgcctatg
     3841 aggtaacggc ggtccgcggc attctcaacg gcacctgcaa tttcatcctg acgcagatgg
     3901 aggccacggg ccggcccttc gccgaagtgc tggccgaggc gcagcgcctg ggttacgccg
     3961 aagcggaccc gacgctcgat attggcggtg gcgacaccgc gcacaagctg acgctgttgg
     4021 cgagcctcgc cttcggcacg gtgccggatt tggatggcgt cgcaatgacc ggcatcgata
     4081 aggtcagccc cgccgatatc gcctttgccg gcgagttcgg ctaccggctg aagctgctcg
     4141 gcgtcgcacg ccgtacggtc aacgggatcg agcagctcgt ccatccggcg atggtgccgg
     4201 ctcgcacacc tctggccgac gtggagtcga gcttcaatgc cgtctcggcc gatgccggcg
     4261 acgcaggaac attctttttc cagggtcgcg gcgcgggtcg cgggccgaca acctccgcgg
     4321 ttgtttcgga tctggtcgat gtcgcccgcg gagcctttgg accggttttt ggacgccccg
     4381 cggcggaact cgcccaaccc aggcgcgagc cggccgaagc gcgtatgagc gcgtattatc
     4441 tgcgcttcca ggttctcgac gttccgggtg tcatggcgga gatctcgcgg catctcgcta
     4501 atgagcgggt ctccatcgag agcatgatcc agcgcggacg cgcgcccggt gagccggtcg
     4561 ccatcgtcat gatcacccat gaggcgcagc atgttgccgt gatgcgcgcc ttgaaggcga
     4621 ttgccgcatc cgacaaagtt tttgagatgc cgtgcatgat tccaatggaa acggcgtagg
     4681 attttactcg gagcgaatcg catgtcccga cctttggatc gtattcttgc gctggaagcg
     4741 gtgcgcgtga cggaagccgc ggcgatccgc gctttcaagg tggctgggcg cggcgatgag
     4801 cacgcagcgg accaggccgc ggtggaaggc atgcggtggg cgttcaatct cctgcccatt
     4861 gacggcaccg tggtgattgg cgagggggag cgcgacgaag ccccgatgct gttcatcgga
     4921 gaaaaagtcg gctcgggcgg gttgaaggtg gacattgcgc tcgatcccct ggaaggcacg
     4981 gcgctttgtg ccaaggggct tcccaacgcg atcgccgtca tggcgatggc ggaggccgga
     5041 accttgttga acgcacccga cgtctacatg gacaagatcg ccatcggtcc cggttttcct
     5101 gaaggcacca tcgatttgga tgccgacccc gccgacaaca tcatcgcgct agccaaggcc
     5161 aaaaacgtga agccggagcg cattcgtgcg ctggtgcttg accgccagcg tcatgagaag
     5221 atcatcgcga gcatccgcga ggccggggcc tcggtgcaac tcatcaccga tggcgatgtc
     5281 gccggggtca ttgccacgac cgaaccggaa accaccggta tcgacattta tatgggacag
     5341 ggcggggccc cagaaggcgt gcttgccgcc tcggcgctgc gctgcgtcgg cgggcagctt
     5401 cagacgcggc tggttttccg caacgatgcc gaacgtcaac gcgccgagcg gtggggcatc
     5461 aaggatctga accgcaaata ccggctgacc gatctggcgt ctggagacgt ggtgttttcc
     5521 gccacgggcg tgacggatgg ttccatgctg gagggcgtgc atgtccatgc cacctatgtg
     5581 acgacccatt ccatcgtgat gcgctcggcg accaagacgg ttcgctggat caagatgcgc
     5641 cacccgctcg acgatgctgg ctgaggcatc gccacggatt ttccaacaca ccgatgcggc
     5701 cccgcaggaa accgcgtccg gcttcgtgct ggatgtggcg tcttcattcc tgggccgcgc
     5761 ctggcgcatg cggccctatg atctggcgcg catgcgcgaa cttgagtgga gcggcctgaa
     5821 tcccgccctg gcgcagattc tggcatcgcg cggggtgcac agggacgttg cggaaagttt
     5881 tctcgatccg cgactgaaga cctctctgcc ggacccctat tgcttcgcca atatggaacg
     5941 ggcggcgagg cgtttcgcgg cggcagtgat acagggtgag atcatcgcca ttctcggcga
     6001 ttacgatgtc gatggcgcat gcgcggccgc tttactgcta cgattcctgc gcggaatgaa
     6061 ccgcgaggcg ctcctgtatg ttccggatcg gatgacggaa ggctatgggc cttcggtggg
     6121 tgcggtcgca tcgcttcgcg cgcaaggagc aaacttgctc gtcacggtgg attgtggggc
     6181 cgcggcacac gaggccctgc aagccgctcg tgattgcggc ctcgatgcga tcgtgcttga
     6241 ccaccacgcg gttgacgtca atccgcctgc cttcgcgcat gtgaatccca atgggccgga
     6301 cgattcctgc ggcatcacct atgtgtgcgc ggccgggctt gtttttatct tcctcgtcgc
     6361 cgtccagcgg atcttgcgcg aagaaggatg gtttgcatcg cagggcctgg acgaaaccaa
     6421 tctcctcgac cagctcgata ttgtggcact cgcgaccatt gccgatgtcg tcccgctcaa
     6481 gggcatcaac cgtgccttcg tgcgtcaggg tctgcgcaga ctggagcggc tggaacgccc
     6541 cggtctggca gcccttgcgc ggctggcttc ggccgaaccg ccttttacgg cctttcatct
     6601 cggcttcgtt ttcggtccgc gcatcaatgc gggcggccgc gtcggacgtt gcgatctcgg
     6661 cgcacgcctc ttggcaagcg atgatccggc ggaagcctgc gcccttggct tggaactcga
     6721 tcgtcacaat cgcgaacgcc gggcgatcga agcgctgatt ttggaatcgg ccgaagccag
     6781 cgctcaggcg caaagccatt cccccttcct tctcgtcagc ggcgatggct ggcatccagg
     6841 cgtggtcggc atcgttgccg gacggctgaa ggagcgtcat gcgaagccgg tattggtggc
     6901 gggcttcgag gccggtggcg agaccgcgat aggccgtggt tcggcgcgct cggtggccgg
     6961 cgtcgatctg ggtgccatca tcaggacggc acgcgccgaa gggctgttgg agaccggcgg
     7021 cggccatgcc atggcggcgg gcttttcgtt gaagcgggag cagcttccgg ctttcgggga
     7081 tttcttgaac cagcagatcg agccgcagcg ctccatgatc acgctggcga gcgagatgac
     7141 gggcgacgcc ttgttgtcgc cgtccggggc tacgctcggc ctgctcgacg agatggatcg
     7201 cgccgggccg tatggagccg ggaatcccga acctttgttc ttcatgcctg acatgctcgt
     7261 ggtctatgcc gggcttgccg gcacgaacca tgtccgggtg cgtctggtcg gacgggacgg
     7321 gcaggggatc ggcgccattg cattccgcgc gggcggctcg ccgctgggag aagggctgct
     7381 caaggcccgc ggcgcgcgcg tccacgtgac gggcaggctc aaacgggacg attacgatgg
     7441 cgtggccaaa gttcagctcc atatcgagga cgcggccccg gccgagccgt aacggtgctt
     7501 gccatcggga ggttcggcca ttatgacgcc tttccggcca gaccagccaa gcgcccttcg
     7561 tctatcggtt aggactcaag attttcaatc ttgcaagagg ggttcgactc ccctagggcg
     7621 cgccaatgtt cagctaaggc gctgacattc cacgaagttc aggcattaca gtgacttacg
     7681 gaccaccgaa gggtatcatt cgggggtatc aatggcgcga attttgatgg gcgttgtcag
     7741 aaaaaaacag acagggatgt acaccgcgcg gaagcgtgtg ccaccccggc tgcaagcagc
     7801 ggttgggaag ctgagtgatc cacccagaga ccgcgtggcg tggcttcaga agtcactggg
     7861 aacgaaggac ctaagccggg cgaacctgct tggtaagcgc gtgctgatgg acttcgacaa
     7921 gatcatagcg cgtgcggagg catccctggc tcctcctccg atgcaggctg acttgtccga
     7981 ccgggagatt gaacagatcg cctcctactt ttttgccgaa gtcttggagg aggatgagga
     8041 ggctaggacg cacggtctat cggatgagaa gcgccccggt atcgatcctg agttcggatt
     8101 gtccgcgaga cagatggagg agttcacgga gagcagggac ggcgtgcttg ccgcctcgca
     8161 gcagcagttg gcgcgcgggg atacgtctac tatccaaagc gaagtagagc gcgtgcttga
     8221 ggactttggt atctcccttg accgcaagtc agtagcgttt cgcaagctta gcatggccat
     8281 gctgcgagag ttcgtgcgag ccatgaaggc tttgcaggac agggacaagg gagagcctat
     8341 cccaacgccc aaggcgcgca tgcctcctgt tcaagcggct gtagcgggtt ctggcgagac
     8401 cctgacggca gcgttgaagg gttgggagaa agcccgcgca ccatcgccga atacgctgca
     8461 agagtttcag tacggtatag gccggttcgt cgaattccac ggtgacatgc cagtagtgaa
     8521 gatcacacgg aagcacgttg cgcagtaccg tgaagccctt caggacatac ccaagcggcg
     8581 cagtggtaaa ctgctgaagg cttctctgcc tgaactggtc gaatggacca agcaacatcc
     8641 gaccgcgcag cggctctcgg caggcaccgt gaacaagctg cttggtgcag ttcagaccat
     8701 agggatttgg gcacgggaca acggagtaat ccccgacgat gtgcagtggt ccgatccgtt
     8761 ctcaaatatg cgcctggacg agaaagagcc ggaacgagcg ccgtgggacg tgcctgaatt
     8821 gaacaccctt ttcgcgtctc ctgtgttcac tgcgcacaag cggccccaag cgggaggagg
     8881 ggaggctgca ttctggcttc cgctgctcgg cctattcacc ggtgcaagac agggcgaact
     8941 tgggccactg cgtacagggg acatagcaac ggacgcgacg acggacattg tgtcgatctc
     9001 catcacggaa gacgcgacga agaacaagcg ccttaagacg agaagctcgc gccggacggt
     9061 cccagtgcat cctgaattag cccgccttgg ctttctacgc ttcgtggacg cacggcgtga
     9121 gaaggacggg caagatgcgc cgctctttcc attgctcaag cctggaccga agggaagcgt
     9181 tgcagagtcg tggtcgaagt ggtttggccg gtacattcgc tccatcggga tcaacgatag
     9241 ctcgcgcgta tttcactcat tccggcattc gttcaaggac gggctgcgtg cagcaggcgt
     9301 tggcgaggac atcaacgacg cgttgacggg gcatagtaac gccagcgtgg gacgctcgta
     9361 tggtgccaag gatagtgtgc gaaggtttgg gctgaacagg ctcgctgcgg cggtcgcggg
     9421 tgtgacgtat gagggggtga agctgcctta atcggtaaac ccttcttcag cccggcccgc
     9481 catccccacg acgtgtacgg cgtttccaat tacatcgcga tgggcgctgg tgaccttggg
     9541 tggccttggg ggaagccaga agttaacgct ctggatgggt atggtaggtg atcatcaaca
     9601 tcgccatcag gacggctgac agtaaccccg cgagtaagcc caataaggcc ggggatgttt
     9661 ccgcgcctac gaagcccatg aggtcatgag cagctaggct gccagcaata gatgcaatcg
     9721 ctgcgttgac gacgagcacc attggaactt ttgactcatc cttgcccttg cctgtcaaat
     9781 cgcttccaac cgcgaccccc atttcgcgtc caataatgac ccctgagtat gggcgttcga
     9841 cagtccacgg ggggacccgc gcgcgcagcg tagcccgtcg ggcttagcgc agcggagcgc
     9901 gtggggggtg cc
//
LOCUS       DASYEG010000175         3446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3679323, whole genome shotgun sequence.
ACCESSION   DASYEG010000175 DASYEG010000000
VERSION     DASYEG010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3679323"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1814)
                     /locus_tag="VGM72_07955"
     CDS             complement(27..1814)
                     /locus_tag="VGM72_07955"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006581.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HEY4265235.1"
                     /translation="MNKMNNGPTVAADAAELTGSEMVIRALKDQGVRHIFGYPGGAVL
                     PIYDALFQDNELVHVLVRHEQGAVHAAEGYARSTGKPGVVLVTSGPGATNAVTGLTDA
                     LMDSIPLVVITGQVATHLIGNDAFQECDTVGITRPCTKHNWLVRDVKDLPRVLHEAFE
                     IATSGRPGPVVVDIPKDVQFKKGAYIGPDQVRHRTYRPRIEADEDAIRQAVRMMAAAK
                     RPVFYTGGGIINSGPEASTLLRELAKLTGFPITSTLMGLGAFPASDPKWLGPIGMHGT
                     YEANLAANRCDLLIALGSRFDDRVTGRVDAFSPGSKKIHLDIDPSQINKNIRVDLPII
                     ADAAHALRAMIGAWKDEKLSADPALPAWWKQIGEWRGKNSLSFRNSAKVIKPQYAIDR
                     LYALTRGRETYITTEVGQHQMWAMQRFKFEEPNHWMTSGGLGTMGYGLPAAVGVQMAH
                     PDALVIDIAGEASVQMTMQEMSTAVQYDLPIKIFILNNEYMGMVRQWQELLHGGRYSH
                     SYSEALPDFVKLAEAYGAVGLRAQTPDELDAKIQEMIAVKRPVLFDVRVDPKENCYPM
                     IPSGAAHDQMILSDAEDEPTVTEEGKMMV"
     gene            complement(2015..2929)
                     /gene="miaA"
                     /locus_tag="VGM72_07960"
     CDS             complement(2015..2929)
                     /gene="miaA"
                     /locus_tag="VGM72_07960"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEY4265236.1"
                     /translation="MSVDVVLIAGPTASGKSHLALELAARIDATIINADSMQVYGELR
                     TLTARPSSAEEMRAPHRLYGHVSVNTRYSVGRYLEEAAGALAEARGAKRVAIFAGGTG
                     LYFDALTNGLSPIPPVPPEIRDTTRARFERIGRDGFFAELMVRDPATASKLRPSDTQR
                     ILRAMDVLEASGRPLSQWQKLAGKPALDGLRMQCFVIAPPREVLVERIERRFETMIKQ
                     GALEEARPLMGLDPTLPAAKALGLPQLRRHLAGEITLADAIAGAQLATKQYGKRQMTW
                     FRNRMKGWHWVAETDLSNFVTSMARDLS"
     gene            3039..>3446
                     /locus_tag="VGM72_07965"
     CDS             3039..>3446
                     /locus_tag="VGM72_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265237.1"
                     /translation="MPLPSVLNVVAGPHGLDDDAARAIHATAKTAVNAGEIDWLAPAH
                     AFDLAFEGDPDSLRAALRPAVSDDWLLDFNIVPAAGRRKRLLIADMDSTIIGCECIDE
                     LADIAGIKPKVAAITERAMRGELDFPSALRMRVA"
BASE COUNT          635 a         1121 c         1017 g          673 t
ORIGIN      
        1 ttcgatatgc gtgtctttct ggatcatcac accatcatct tgccttcttc ggtcaccgtg
       61 ggctcgtcct cggcatccga aagaatcatc tggtcatggg ccgcacccga ggggatcatg
      121 ggatagcaat tctccttcgg atcgacccga acgtcgaaca gaacgggccg cttcaccgcg
      181 atcatctcct ggatcttggc gtccagctcg tcaggcgtct gtgcccgcaa tccgaccgcc
      241 ccataggctt ccgccagttt cacgaaatcg ggcagcgctt cggaatagga atgcgaatag
      301 cgcccgccat gcagcaattc ctgccattgg cgcaccatgc ccatatattc gttgttcagg
      361 atgaagatct tgatcggcag atcgtattgc accgccgtcg acatttcctg catcgtcatc
      421 tgcaccgacg cttcgccggc aatgtcgatg acgagcgcgt cgggatgggc catctgcacg
      481 cccacggccg ccggcaagcc ataccccatc gtgccgagcc cgcccgacgt catccaatga
      541 ttgggttctt cgaatttgaa gcgctgcatc gcccacatct gatgctggcc gacctcggtg
      601 gtgatgtagg tttcgcgccc ccgcgtcagc gcgtagaggc ggtcgatcgc atattgcggt
      661 ttgatcacct tggccgaatt gcggaagctg agcgaattct tgccccgcca ttcgccgatc
      721 tgtttccacc atgccggcaa tgcgggatca gcggaaagct tctcgtcctt ccacgcgcct
      781 atcatcgcgc gcagcgcgtg cgccgcatcg gcgatgatcg gcagatcgac acggatgttc
      841 ttgttgatct gcgagggatc gatgtcgaga tggatcttct tcgaacccgg cgaaaacgcg
      901 tctacccggc ccgtgactcg gtcgtcgaag cgcgagccca gcgcgatcag aagatcgcag
      961 cgattggccg ccagattcgc ctcataggtg ccatgcatcc cgatcggccc gagccatttc
     1021 ggatcggaag ccggaaaggc gcccaatccc atcaacgtcg aggtgatcgg aaaaccggtc
     1081 agcttcgcca gctcgcgcag aagcgtgctc gcttccggcc ccgaattgat gatgccgccg
     1141 ccggtataga acaccggacg cttggccgcg gccatcatcc gcacggcctg gcggatggcg
     1201 tcttcatcgg cttcgatccg cggacgatag gtgcgatggc gcacctgatc ggggccgata
     1261 taggcgcctt tcttgaactg cacgtccttg gggatgtcga ccaccaccgg tccgggccgt
     1321 ccgctcgtgg cgatctcgaa cgcctcgtgc agcacgcgcg gcaggtcctt cacgtcgcgc
     1381 accagccaat tgtgctttgt gcagggccgc gtgatgccga ccgtgtcgca ttcctgaaac
     1441 gcgtcattgc cgatgagatg cgtcgcgacc tggccggtga tgaccaccag cggaatggaa
     1501 tccatcagtg catcggtcag gcccgtcacc gcattcgtgg ccccgggacc gctggtgacc
     1561 aggaccacgc ccggcttgcc ggtggaacgc gcatagcctt ccgcggcgtg aaccgcaccc
     1621 tgctcgtggc gcaccagcac atgcacaagc tcattgtcct ggaaaagcgc atcgtagatt
     1681 ggcagaaccg cgccgccggg atagccgaaa atatggcgca caccctgatc cttgagggcg
     1741 cggatcacca tttccgaacc tgtcagctcg gcggcatctg ccgccacggt cggcccattg
     1801 ttcatcttgt tcatttcgcc ctccgaacag gcgcaactcg gtacttggac tcaaacgaaa
     1861 ggggcgtcgc cgccccttgg ataaccgcgt cgaggaacgg agccctaggg cctcatcctc
     1921 cgccgcggcc aggttacgag tacatgcgtc acaagcttta tcctgtcttg atgcgattga
     1981 ccttagttgc atcgcagcaa ggggtcaaga aacttcacga aagatcacgc gccattgatg
     2041 taacaaaatt gctcaaatct gtctctgcga cccaatgcca ccccttcatc cggttccgga
     2101 accacgtcat ctgccgtttt ccatattgtt tggtggccaa ttgtgccccc gcaatcgcgt
     2161 ccgccagcgt tatttcgccc gccagatgac gccggagttg cggcaggccc agagccttgg
     2221 ctgccggcaa cgtaggatcg agccccatga gcgggcgcgc ttcctccaac gcgccttgct
     2281 tgatcatggt ctcgaagcgc cgttcgatgc gctccaccag cacctcgcgc ggcggagcga
     2341 tgacgaagca ctgcatgcgc aggccatcga gagccggctt gcctgccagc ttctgccatt
     2401 gcgacagcgg ccggcccgag gcttccagca catccatggc gcgcaggatg cgctgcgtat
     2461 cggatgggcg aagcttggac gccgtcgcgg gatcgcgcac catcagctcg gcgaaaaaac
     2521 catcgcggcc gattctttcg aagcgcgccc gtgtggtgtc gcgaatctcg ggcggcacgg
     2581 gcgggatggg ggaaagaccg ttcgtcaacg catcgaaata tagccctgtg ccgccggcga
     2641 agatcgcgac gcgtttggcg cctcgcgctt cggccagcgc gcctgcggct tcttccagat
     2701 agcggcccac ggaatagcgc gtgttgacac tcacatgccc atagagccga tgcggcgcgc
     2761 gcatttcctc cgccgatgag ggccgcgcgg tcagcgtgcg cagctcgcca tagacctgca
     2821 tcgaatccgc attgatgatg gtggcatcga tgcgcgcggc cagttcaagg gcaaggtgcg
     2881 acttgccgct cgcagtcggc cctgcgataa gcaccacgtc cacggacatg actcctcccc
     2941 ttcgacaggc tcagggtgag gaatagaaaa acttcctcat gctgagctag tcgaagcatg
     3001 ggggttaaat caacttgccc ggaaaggcgc aattgcccgt gcccttgccc tctgtgctca
     3061 atgtggttgc cggtccgcac gggcttgacg acgatgccgc ccgcgcaatc cacgccaccg
     3121 cgaaaaccgc ggtgaacgcg ggtgagatcg attggctggc gccggcgcat gccttcgatc
     3181 tcgcattcga gggcgacccg gattcgctgc gcgctgccct gcgcccggcg gtttccgacg
     3241 attggctgct cgatttcaac atcgtaccgg cagcgggccg ccgcaagcgg ctgttgatcg
     3301 ccgatatgga ttccacaatc atcggctgcg aatgcatcga cgagctggcg gacattgcgg
     3361 gcatcaagcc gaaggtcgcg gccatcaccg aacgcgccat gcgcggcgag ctcgattttc
     3421 ccagcgcctt gcggatgcgc gtcgcg
//
LOCUS       DASYEG010000176         2065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2086495, whole genome shotgun sequence.
ACCESSION   DASYEG010000176 DASYEG010000000
VERSION     DASYEG010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2086495"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VGM72_07970"
     CDS             <1..892
                     /locus_tag="VGM72_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265238.1"
                     /translation="ARLLDTPAGNPTIGGVLCADINGAAAIRYGRARDSLLGVRAANG
                     LGEAYKAGGKVVKNVTGFDLPKLMCGAMGTLGVLTEVTLRVYPKPQQTASLIVRDVNV
                     EEGFALLRRVWQSPLEATALAYIPASIPLTQLGYVGEGAALIRLEGAHEPLKEKIAAL
                     LAILPSAQRIEASSIFGLISSGGIFVDQSCDLWRIAVAPSKAAGIVRQMGSSIFAADW
                     AGGSLWIGLEPGGNGAELRTHCEKAGGQAILVKADEDTRQRVAPFPPEPPARRSLTKA
                     VKAAFDPQNIFNPGRMWKAV"
     gene            892..>2065
                     /gene="glcF"
                     /locus_tag="VGM72_07975"
     CDS             892..>2065
                     /gene="glcF"
                     /locus_tag="VGM72_07975"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: protein motif:HMM:NF008434.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEY4265239.1"
                     /translation="MRTNFSIAQLADPHLADAQKNLRACVHCGICTATCPTYVLLGDE
                     RDGPRGRIVMMQAMLETDGAPTPETVHHLDRCLSCLGCRTACPSGVDYPRLIDAARVH
                     VETHYTRPWNERALRWLIANVLTRPELSRTMLMLAKASEPLHFLLPGRLRVMARKGAA
                     MKIGSVTSPIEASPGTKRIALMPGCVQKALAPEIDEAVARLLMRRGFSLVPLDGAGCC
                     GSLVHHLGRENDAKAWAMRAIESFERAGEDFEAVLITATGCSSHLKDLAHLFADDPVW
                     QPRAMRFAAKVKDFCELISPRGAENSHRLRVAYHAPCSLQHGLGLLGANEKLLTAAGF
                     DVAAIPEGHLCCGSAGSYSILQPELADALRSRKLANIATVEPDVIATANIGCLEHLS"
BASE COUNT          360 a          609 c          677 g          419 t
ORIGIN      
        1 ggcaaggctg cttgatacgc ccgccggcaa cccgaccatc ggcggcgtgc tgtgcgccga
       61 tataaatggc gctgccgcca tccgctatgg ccgggcgcgg gattccctgc ttggtgttcg
      121 cgcggcgaac ggcttgggcg aagcttataa ggccggcggc aaggtggtga agaacgtcac
      181 cggcttcgat cttcccaagc taatgtgcgg cgcgatggga acgctgggcg tgctcaccga
      241 agtgacgctg cgggtttatc ccaagccgca gcaaaccgct tcgctcattg tgcgtgacgt
      301 gaatgtggaa gagggcttcg cgctgctgcg ccgggtgtgg cagagcccac tggaagcgac
      361 cgcgcttgcc tacatccccg catcaatccc gctgacccag ttgggctatg tcggcgaagg
      421 cgcggcgctg atccgtctgg agggcgcaca tgagccgctg aaggagaaga tcgcggcact
      481 gcttgcgatc ctgccgtccg cgcagcggat cgaagcatcc agtattttcg gtttgatctc
      541 cagcggcggg atcttcgtgg accagagttg cgatctgtgg cgcattgccg ttgcgccttc
      601 gaaggcggcg ggcatcgtcc ggcaaatggg ttcttcgata tttgcggccg attgggcagg
      661 cggatcgctc tggatcggac tcgaacccgg cggcaatggc gcggaactgc gtacgcattg
      721 cgagaaagcg ggtgggcagg caattctcgt aaaagccgat gaagatacac gtcagcgcgt
      781 tgcgccgttc ccaccggagc cgcccgcgcg gcggtcgctg acgaaagcgg tgaaagccgc
      841 tttcgatccg cagaatatct tcaatcccgg ccgcatgtgg aaagcggtct gatgcgcacg
      901 aatttctcca tcgcgcagct tgccgatccg catctggccg atgcgcagaa gaacctgcgc
      961 gcctgcgtgc attgcggcat atgcacggcg acatgcccga cctatgtgct gctcggagac
     1021 gaacgcgacg ggccgcgcgg ccgcatcgtg atgatgcagg ccatgctgga aaccgatggc
     1081 gcgcccacgc cggaaacggt gcatcatctg gatcgctgcc tgtcctgcct cggctgccgc
     1141 acggcgtgtc cctccggcgt ggattatcca aggctgatcg atgcggcgcg tgtgcatgtg
     1201 gaaacgcact atacgcgtcc atggaatgag cgtgcccttc gctggctgat tgcgaatgtg
     1261 ctcacgcgtc ccgagctctc gcgcacaatg ctgatgttgg cgaaggcgtc ggaacccctg
     1321 cattttcttt tgcctggtcg cttgcgcgtg atggcccgca agggtgccgc catgaaaatc
     1381 ggctctgtga cttcgccgat cgaagcttcg cccggtacga agcgcatcgc gctgatgccg
     1441 ggctgtgtgc aaaaagcgct ggcgccggaa atcgatgaag ccgtggcgcg attgcttatg
     1501 cgtcgtgggt tttcccttgt gccgctagac ggcgcgggct gctgcggctc gctggtgcat
     1561 catttgggcc gcgagaacga cgccaaagca tgggccatgc gcgctatcga aagcttcgaa
     1621 cgcgccggag aagatttcga ggcggtgctc atcactgcaa cagggtgttc gtcacatttg
     1681 aaggatctgg cgcatctctt cgcggacgat ccggtgtggc aaccgcgcgc catgcgtttt
     1741 gccgccaagg tgaaggattt ttgcgagctc atctcgccgc gtggtgcgga aaattctcac
     1801 cggttgcggg tcgcttatca tgcgccatgc tcgctgcaac acgggcttgg tctattgggc
     1861 gcaaacgaaa agcttttgac ggcggcgggt ttcgatgtcg ccgccattcc cgaaggtcat
     1921 ctgtgctgcg gttcggcggg cagctattcg atcctgcagc ccgaacttgc ggacgctctg
     1981 cgcagccgca agttggccaa tatcgcgacc gtggaaccgg acgtgattgc taccgccaat
     2041 atcggctgtc tggagcatct gtccg
//
LOCUS       DASYEG010000177        10299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3968978, whole genome shotgun sequence.
ACCESSION   DASYEG010000177 DASYEG010000000
VERSION     DASYEG010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 10299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10299
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3968978"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VGM72_07980"
     CDS             <1..738
                     /locus_tag="VGM72_07980"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY4265240.1"
                     /translation="AARYIVSAEGARSIVRKECGIEFTGFTYPDRTLNIEVAYDFKQH
                     GYTDRNYISDPEEWSNLFHWKGPPERWRVHFPTAEEEDPDALMAPDAMQKRLQGFLAT
                     GKPFDIVGYNLYTVHQRVAEKFHAGRALLAGDSAHVNSPIGGMGLNSGVHDAFNLAEK
                     FARIWRGEADDALLDLYDRQRRHIAVQHTQLQTIRNKKLLQEKDPKVREANHDALKRA
                     AADPKLAREFLLRTSLISSVREAARIT"
     gene            757..1143
                     /locus_tag="VGM72_07985"
     CDS             757..1143
                     /locus_tag="VGM72_07985"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011578813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY4265241.1"
                     /translation="MSAIERVLTPNAPKPAGHYSQAIAYGDLIFVSGQLPVTLDGKIA
                     ADESFEEQARLALANLLAIVRAAGSSPDRVLKVTVFLAGVEYWPKFNEIYAEAFGEAR
                     PARSVVPVPALHYGALVEIEAIASRA"
     gene            1214..2071
                     /locus_tag="VGM72_07990"
     CDS             1214..2071
                     /locus_tag="VGM72_07990"
                     /EC_number="4.1.3.34"
                     /inference="COORDINATES: protein motif:HMM:NF004864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HEY4265242.1"
                     /translation="MDDSKHATGLSLEAVTDWWESEITDIAPGSIRVRGYRIEDLIGR
                     VSFPAMIWLVMRGELPTKRQADLFGAVLVAGVDHGPQAPSISIARMAITCGIGINGAM
                     ASAVNALGDVHGGAGQQCMGLLADISRRVSGGAALDAAVSAALDAFRSAHGRFIPGFG
                     HRFHAVDPRAARLSELIKEAAREGLVGGQFLAAGEAVQQRLSQGRTTSLPMNVDGITA
                     VMLLELGFPEEMGRGIFILSRSVGICAHAFEQSQRGERIKGPTPPDFGFRYRGVAPRE
                     FPDDADGLS"
     gene            2071..3228
                     /locus_tag="VGM72_07995"
     CDS             2071..3228
                     /locus_tag="VGM72_07995"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY4265243.1"
                     /translation="MADAGSRPLPLSGIRVLEFGHTILGPCCGMVLADFGAEVIKIEP
                     AGGERTRRLKGFGAGYFAFFNRNKKSVILDYKSAEGCAAARALIATSDVLIENMAPDA
                     LTRAGLGPMESRALNPQLIYCSLKGFLEGPYRARAALDEVVQMMSGLAYMTGPPGRPL
                     RAGTSISDMLGGIFGAMGILIALRERDSGRGGATVESALFETAAFLMGQFMAANALSG
                     EPVPPMPARVSAWAVYELFETADAERVFIGITSDSHWRNFCGEFARADLLADAHLATN
                     EMRVMERPRLIPQLIALFKSLAAAEIERRCLAAGLPFAKIARPEDLFEDPQLNANGSL
                     ADTVLAGGIATKLPKLPIRIDGAAFGLRSDPPGAGADTREILDALRARSSR"
     gene            3225..4181
                     /locus_tag="VGM72_08000"
     CDS             3225..4181
                     /locus_tag="VGM72_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEY4265244.1"
                     /translation="MTEIKVGILDDFQNITRTIADWDRLPPHVSVTVFNAHFEGEELI
                     HRIAGFDVLVITRERTPFPRGVVERLPNLKLLVTAGWRNLGIDMAACREHGILVCGTE
                     TGSSPTAELAWGLILSVARHIAEEDAQLRKGQWQSTIGFGLQGKTLGILGLGRLGSQM
                     ARIGNAFDMNVIGWSQNLTPERAREAGATRVEKSELFRASDVLTIHLLLSERTRGLVG
                     HDEIGQMKKSAILVNTARAAIVDETALTAALTAGTIAGAGLDVHSREPLPDGAPILNA
                     PNTVLTPHLGYVTRETYGIYFPQALEDIEAWLQGTPIRIIAD"
     gene            4244..4915
                     /locus_tag="VGM72_08005"
     CDS             4244..4915
                     /locus_tag="VGM72_08005"
                     /inference="COORDINATES: protein motif:HMM:NF040005.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6134 family protein"
                     /protein_id="HEY4265245.1"
                     /translation="MPAQAKGLRILPAALAMAAFALLSPAHSAAPAVQRLTYSVHHSR
                     YGTLGTYTNAVTKSGDMTTVNTEVHIQLSILGVVLYRQEAMRQERWNAGRLVAFHGVT
                     TVNGRSFEMNGAAQGERFVMMSPDGEITAPASVKIANPWSSEVLRGDTLLTPDRGRME
                     NVQVKAAENTSIPINGRAMPAKRYEIDRLDGQKRYEIWMDDGDIPVMFTLYNPNGSVT
                     FTLMP"
     gene            4965..5858
                     /locus_tag="VGM72_08010"
     CDS             4965..5858
                     /locus_tag="VGM72_08010"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEY4265246.1"
                     /translation="MKALTLLILTSAVAGIVAAALLSSSEGPVRVPSGFGETALTKPA
                     TVPKAQAFKDCAQCPDMVAVPAGAFTMGSPPSELYRGAEMQHRVTIAAPFALGKYEVT
                     FAEWDACVADGGCGGYKPDDHGWGRENRPVVDVSWDDAKIYVAWLNKKTGKAYRLPSE
                     SEWEYAARAGATTAFSFGATITSQQANYDGSTAYGEGATGPARKKTMPVGSFPANAFG
                     LHDMHGNVWEWLEDCWSEEYTAASPANGAPYRGGNCAGRVMRGGSWEDYPGDLRAAAR
                     VGSNTDDQSWADGFRVARAME"
     gene            complement(5872..6441)
                     /locus_tag="VGM72_08015"
     CDS             complement(5872..6441)
                     /locus_tag="VGM72_08015"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HEY4265247.1"
                     /translation="MEPEIEVEHHANPTNKRIGILIAIMATVLAFTEMAARNADTDIV
                     RESVEASDIWAFFQAKSIRAAMLRADARALQVETAGRAETDSGQVAKTVADWEAAAAH
                     EDSEPTTGEGRKELAAKAQGIEKHRDERAAAKETYEFASGALELGILLASSAIVTALI
                     PLAFIGAAFGVVGTVLGVLGHFAPHAIGG"
     gene            complement(6450..8507)
                     /locus_tag="VGM72_08020"
     CDS             complement(6450..8507)
                     /locus_tag="VGM72_08020"
                     /inference="COORDINATES: protein motif:HMM:NF006192.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HEY4265248.1"
                     /translation="MKSRWSDSDAQAFIARYRDAGIAPDLALRVYTTRLLGGDPALVL
                     HGGGNTSVKTEVKDLLGDAIEVICVKGSGWDMGDIEPAGLPAVKLAPLRRLLALEKLS
                     DEDMVNYQRIHLLDSAAPNPSVETLLHAFLPHKFVDHTHSTAVLALTDQPAGEEIARE
                     TFGARMAYVPYTIPGFALAKSVAGVYAATPDVQGLILLRHGIFTFAQDARTAYELMIE
                     MVSRAESHIARGKKTLVPVQLPGRLAGVAEIAPILRGSVALENKREDGTAKRQILAFR
                     TNEKIRDYVNGAELARYSQIGVATPDHTIRTKNLPLIVPAPEAGKLDRWADAVRSAVD
                     GFVAHYHDYFARNNARLRGVKKELDPFPRVVLVPGLGLFGLGASAKDAAIAADIAENT
                     VDVIEAAERGSSYRPISEEDQFDVEYWSLEQAKLGKGAEKSLARQVAVITGGGSGIGA
                     ATAKAFAKQGAEVAVLDRDGEAAARVAASIGGHALGLACDVTDADPVTRAFDAIAEKF
                     GGLDILVSNAGAAWQGRIGDVDDAILRQSFELNFFAHQSVAQHAVAIMKAQGTGGCLL
                     FNTSKQAVNPGKDFGPYGLPKAATLFLVKQYALDHGKDGIRANAVNADRIRTGLLTDG
                     MVTARARARGLSESDYMSGNLLGREVTAEDVADAFVFLALARKTTAAVLTVDGGNIEA
                     SLR"
     gene            complement(8605..9648)
                     /locus_tag="VGM72_08025"
     CDS             complement(8605..9648)
                     /locus_tag="VGM72_08025"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEY4265249.1"
                     /translation="MSLSLRVWGPLRILRLCACVSVASAAGAPALNAAPAETPPSDPS
                     KTIFVDLSAPGSAQASPQAAATARPSSVTTFVDLSKGGAPIAMTAPGTPGPFRTPRTQ
                     HIDLSNGAALPAMTEEDQYLPQPEPDRITITADRIADPYEEANRGRFKSHVGLHRYLI
                     DPVERSYIYVVPLPARDGLHNFLTNLETPTVLANNLLKGEWDRAGNTLSRFVVNTTIG
                     IGGIFDFAGDAGIAYRDDDFGATLAKYGVSDSPYLLIPVIGPSNPRDLGGKVVDFIMD
                     PLHFVTLPGGIITSVGHTGLHEVDKRSVDVGELDQLAKTSPDAYAEERAKARAARNAE
                     ITGTSPPAPEFGR"
     gene            complement(9756..>10299)
                     /locus_tag="VGM72_08030"
     CDS             complement(9756..>10299)
                     /locus_tag="VGM72_08030"
                     /inference="COORDINATES: protein motif:HMM:NF013197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265250.1"
                     /translation="AHDEGIPVHVWVDETRPRNQGASLTAFELSSHGVPHAVIADNAG
                     GHLMQAGRVDLAIVGTDRVTRNGDVANKIGTYLKALAAKDNHLPFYVALPSTTIDWTL
                     ASGGLMPIEERVATEVTQVTGRLADGSIASVEITPRGSPAANPGFDVTPARLVTGFIT
                     ERGVCVASEAGLRALFPDGV"
BASE COUNT         1914 a         3266 c         3283 g         1836 t
ORIGIN      
        1 gccgcgcgct acatcgtcag cgccgagggc gcgcgcagca tcgtgcgcaa ggaatgcggc
       61 atcgaattca ccggcttcac ctatcccgac cgcacgctga atatcgaggt cgcttacgat
      121 ttcaagcagc acggctacac ggatcgcaat tacatctccg atcccgagga atggtcgaac
      181 ctgtttcact ggaaaggacc gccggaacgc tggcgcgtgc atttcccgac cgcggaggag
      241 gaggatcccg atgcgctgat ggcgcccgac gccatgcaga agcggctgca gggtttcctt
      301 gccaccggca agcctttcga catcgtgggc tacaatctct acaccgtgca tcagcgcgtg
      361 gcggagaaat tccacgcggg ccgcgctctc ctcgcgggcg attcggcgca tgtgaatagt
      421 cccatcggcg gcatgggatt gaacagcggc gttcacgacg ccttcaatct ggccgaaaaa
      481 ttcgcccgca tctggcgggg cgaagccgat gacgcgctgc tcgatctcta tgaccgccaa
      541 cgccgccata tcgccgtgca gcacacccaa ttgcagacca tccgcaacaa gaagctgctg
      601 caggaaaagg acccgaaggt cagagaggcc aatcacgacg cgttgaagcg cgccgcggcc
      661 gatcccaaac tggcgcgcga attcctgctc cgcacctcgc tcatcagcag cgtgcgcgaa
      721 gctgcccgca taacctgatt tgcaacgagg atattcatga gcgcgatcga acgcgtcttg
      781 acgccaaacg cgcccaagcc ggcgggccat tattcgcaag cgattgccta tggcgatctg
      841 atcttcgtct ccggccaatt gccggtgacg ctggatggca aaatcgccgc cgatgagagt
      901 ttcgaggaac aggcccgtct cgcgctcgcc aacctccttg ccatcgtgag ggcggccggc
      961 agcagcccgg atcgcgtgct gaaagtgacc gtgttcctcg cgggcgtgga atattggccg
     1021 aaattcaacg agatctatgc ggaggcgttc ggggaggcgc ggcccgcgcg ctccgtcgtg
     1081 ccggtgccgg cgctgcatta tggcgccctc gtcgagatcg aagccatcgc ctcgcgggcc
     1141 tgagccgtaa agcggggctg aatttcgccg tgcacaatgg catggtgtcg cgagggttaa
     1201 ccggagcgta cccatggacg attccaaaca cgcgacaggc ctgtctctgg aagccgtgac
     1261 cgattggtgg gaaagcgaga tcaccgacat cgcgccgggc tcgatccgcg ttcgcggcta
     1321 tcgaatcgag gacctgatcg ggcgcgtcag ctttccggcg atgatctggc tcgtcatgcg
     1381 cggcgaactg cccaccaagc gccaggccga tctgttcggc gcggttctcg tggccggcgt
     1441 cgatcacggc ccgcaggcac cgtcgatttc catcgcgcgc atggcgatca cctgcggcat
     1501 cgggatcaac ggcgccatgg cgagtgcggt gaatgcgctc ggtgacgtgc atggcggcgc
     1561 gggacagcaa tgcatgggac ttctcgccga cataagccgg cgcgtgtcgg gtggtgcggc
     1621 actcgatgcc gccgtctcgg ctgcgcttga tgcattccgc agcgcccatg gacgcttcat
     1681 cccgggtttc ggccatcgtt tccatgcggt cgatccccgc gcggcgcgcc tctcggaatt
     1741 gatcaaagag gcggcgcgcg agggtctcgt gggaggccag ttcctcgccg ccggcgaagc
     1801 ggtccagcag cgtctgagtc aggggcgcac gacatccttg ccgatgaacg tcgatggcat
     1861 caccgcggtc atgctgctcg aactcggatt tccggaagaa atggggcgcg gcatcttcat
     1921 cctgtcgcgc agtgtcggga tttgcgcgca cgccttcgag caatcgcagc ggggcgagcg
     1981 catcaaaggg cccacgccgc cggatttcgg atttcgctat cgcggcgtgg ccccccgcga
     2041 attcccggac gatgcggacg gcctgtcctg atggcggatg cgggctcaag accgctgccg
     2101 ctttcgggca ttcgcgtgct cgaattcggg cacacgatcc tggggccctg ctgcggcatg
     2161 gtgctggcgg atttcggcgc ggaggtgatc aagatcgagc cggcgggtgg ggaacgcacc
     2221 cgccgcctga aagggtttgg cgcgggctat ttcgcgttct tcaaccgcaa caagaaaagc
     2281 gtcatcctgg attacaaatc cgccgaaggg tgtgcggccg cgcgcgccct gatcgcgaca
     2341 tccgacgtgc tgatcgagaa tatggcgccg gacgcgctca cgcgcgcggg tctggggccg
     2401 atggaatcgc gcgcgctcaa tccgcaactg atctactgct cgctgaaagg cttcctcgag
     2461 gggccctata gggcacgcgc ggccctcgat gaagtggtgc agatgatgag cgggcttgcc
     2521 tatatgaccg gaccgcccgg acggcctttg cgcgcaggca cttcgatttc ggacatgctg
     2581 ggcggcattt tcggtgcgat gggaatcctg atcgcgttgc gcgaacgcga ttctgggcgc
     2641 ggtggagcga cggtcgaaag cgcgctgttc gagaccgccg ccttcctgat gggccaattc
     2701 atggccgcga acgcgctctc cggtgagccg gtgccgccga tgcccgcgcg tgtcagcgcc
     2761 tgggccgtgt atgaactgtt cgagacggcg gacgctgagc gcgtcttcat cggcatcacc
     2821 agcgattccc attggcggaa tttttgcggc gaattcgcgc gcgccgattt gctggccgat
     2881 gcacacctcg ccaccaatga gatgcgcgtg atggaacggc cccgcctcat tccccaattg
     2941 atcgcgctgt tcaaaagcct cgccgccgcc gagatcgaac gccgctgcct ggccgcaggg
     3001 ctgcctttcg cgaaaatcgc gcggcccgag gatttgttcg aggacccgca gttgaatgcc
     3061 aatggcagcc tcgccgatac cgtccttgcc ggcggcatcg ccaccaagct tccgaagctt
     3121 cccatccgca tcgacggcgc ggcgttcggt ctgcgcagcg atccgcccgg cgccggtgcg
     3181 gacacgcgcg aaatcctgga cgccttgcgc gcacggagca gcagatgaca gagatcaaag
     3241 tcggcatcct cgacgatttc cagaatatca cgcggacaat cgccgattgg gaccgcctgc
     3301 cgccgcatgt ctccgtcacg gtcttcaatg cgcatttcga gggcgaagaa ttgatccacc
     3361 gcatcgcggg tttcgacgtg ctcgtgatca cgcgcgagcg cacgcccttt ccacgcggcg
     3421 ttgtggaacg ccttcccaat ctgaagcttc tcgtcaccgc gggctggcgc aatctcggca
     3481 tcgacatggc cgcctgccgg gagcacggca tcctcgtctg cggcaccgaa acgggaagct
     3541 cgcccacggc ggaacttgcc tgggggctga tcctcagcgt cgcccggcac atcgccgaag
     3601 aagatgcgca actacgcaaa ggccaatggc aatccaccat cggtttcggg ctgcaaggca
     3661 agacgctggg catcctcgga ctcgggcggc tgggctcgca gatggcgcgc atcggcaacg
     3721 cgtttgacat gaatgtgatc ggctggagtc agaatttgac gcccgaacgc gcgcgcgaag
     3781 caggcgccac gcgggtggaa aaatccgaac tcttccgcgc ctcggacgtg ctgacgatcc
     3841 atcttctgct cagcgaacgc acgcgcggcc tcgtcgggca cgatgaaatc ggccaaatga
     3901 agaagtccgc gatcctggtg aacaccgcgc gcgcggccat cgtggacgag accgcactga
     3961 cggctgcgct gaccgcaggc acaatcgcgg gcgcgggtct ggatgtccat tcccgcgagc
     4021 cgctgccgga tggtgcgccg atcctgaacg cgccgaacac cgtgctgacg ccgcatctcg
     4081 gctatgtcac acgcgagacg tatggaatct attttcccca ggcgcttgag gatatcgagg
     4141 cctggcttca gggtacgccc atccgtatca tcgcggatta gggacggcgg ggagccctaa
     4201 gttgttagaa gcgttattgg gagccaacgc acgggagctg ccaatgccgg cccaagccaa
     4261 gggattgcga attcttccgg cggcgctcgc gatggctgca ttcgcgctgc tgtctcccgc
     4321 tcattcggcg gcgcccgccg tccagcggct gacctattcg gttcaccatt cgcgttatgg
     4381 cacattggga acctatacca acgcggtgac gaaatccggc gacatgacca ccgtcaatac
     4441 ggaggtccat attcagcttt cgattctggg cgtcgtgctc tatcgacagg aagcgatgcg
     4501 acaggaacgc tggaatgccg ggcgcctggt cgcattccat ggcgtgacca cggtgaacgg
     4561 ccggtctttc gagatgaatg gcgccgcaca gggcgagcgt ttcgtcatga tgtcgcccga
     4621 cggtgagata acagcgccgg ccagcgtgaa aatcgccaat ccctggtcgt ccgaggtgct
     4681 gcgcggcgat acgcttctga cccccgacag aggacgcatg gaaaatgtgc aggtgaaagc
     4741 cgccgagaac acctccattc ccatcaatgg ccgcgcgatg cccgcaaagc gttatgaaat
     4801 cgaccggctc gacggacaga aacgctacga aatctggatg gacgacggcg atatcccggt
     4861 catgttcacg ctctacaatc ccaatggcag cgtgactttc actctcatgc cttgagagaa
     4921 ccgccgaggc acagtaaagt gcgcaacagc accgtcctga atccatgaaa gcgctcaccc
     4981 tcctcatcct cacctcggca gtggccggca tcgttgccgc cgcgctgctg agttcgagcg
     5041 aggggcccgt gcgcgtccct tcgggttttg gcgaaaccgc gctcaccaaa ccagcgacag
     5101 tgcccaaagc ccaagcgttc aaggactgtg cgcagtgccc cgacatggtg gccgttccgg
     5161 ccggcgcgtt caccatgggc tcgccgccgt ccgaacttta tcgcggcgcg gagatgcagc
     5221 atcgcgtgac catcgccgcg cccttcgcgc tcggcaaata tgaggtcaca ttcgccgagt
     5281 gggatgcctg cgtcgccgat ggcggctgcg gcggctacaa accggacgat catggctggg
     5341 ggcgggaaaa ccgtcccgta gtggatgtga gttgggacga cgccaagatc tatgtcgcat
     5401 ggctgaacaa gaaaaccgga aaagcctacc gcctgccctc cgaatccgaa tgggaatatg
     5461 ccgcacgcgc gggcgccacg acggcgtttt cgttcggggc gacgatcacc agccagcaag
     5521 cgaactatga cggcagcacg gcctatggcg agggcgccac cggaccggcc cgcaagaaaa
     5581 cgatgccggt gggaagcttt cccgccaacg cgttcggcct ccacgacatg cacggcaatg
     5641 tctgggagtg gctggaggat tgctggtccg aggaatatac ggccgccagc cctgcgaacg
     5701 gtgctcccta tcgcggcggc aattgtgcgg gacgcgtgat gcgcggcggc tcgtgggagg
     5761 attatcccgg cgatctgcgc gcggccgcgc gggtcggcag caacaccgac gaccaatcct
     5821 gggccgatgg attccgcgtc gcacgggcga tggaataaat cgcgtaccgc gttacccgcc
     5881 gatggcgtgc ggggcaaaat gtccgagcac gccgagaacg gtgcccacga cgccaaaggc
     5941 ggcgccgatg aatgcgagcg gtatcagcgc cgtcacgatg gctgaagacg cgagcaggat
     6001 tcccagctcc agcgcaccgc tggcgaattc ataagtctcc ttggcggcgg cgcgctcgtc
     6061 gcgatgtttc tcgatccctt gcgccttcgc ggccagttcc tttcggccct cgcccgtggt
     6121 gggctcggaa tcttcatgcg cggcggccgc ctcccaatcg gcgaccgttt tggcgacctg
     6181 tccggaatcg gtttcggcgc gtcccgccgt ctcgacctgc agcgcacggg catcggcgcg
     6241 caacatcgcc gcacggatag atttggcctg gaagaacgcc cagatgtccg aagcttcaac
     6301 ggattcgcgc acgatgtcgg tatcggcatt gcgcgccgcc atttcggtga aggcgagtac
     6361 cgtcgccatg attgcgatca ggataccaat gcgcttgttg gtggggttgg cgtgatgttc
     6421 gacttcgatc tccggttcca tgattcactc tatctgagag aggcctcgat attgccgccg
     6481 tcaacggtga gaaccgcggc cgtggtcttc cgggccaatg cgaggaagac gaacgcgtcg
     6541 gcgacatcct cggcagtgac ttcgcgcccc agaagattgc cgctcatata gtcggattcc
     6601 gaaagccccc gcgctcttgc ccgcgccgtc accatgccgt ccgtcagaag gcccgtgcgg
     6661 atgcggtcgg cattgaccgc attggcgcga atcccgtcct tgccgtgatc cagcgcatat
     6721 tgcttcacca ggaacagggt cgcggctttg ggcaatccat aggggccgaa atccttgccg
     6781 gggttcaccg cctgtttgga ggtgttgaac agaaggcaac cgcccgtccc ttgcgccttc
     6841 atgatggcga ccgcgtgctg cgccacactc tgatgcgcga agaaattcag ctcgaaggat
     6901 tgacgcaaaa tggcatcgtc gacatcgccg atgcgcccct gccacgccgc acccgcattg
     6961 gagacgagga tatcgaggcc gccgaatttt tcggcaatcg cgtcgaaggc gcgggtgacc
     7021 ggatcggcat cggtcacatc gcaggcaagt ccgagcgcat ggccaccgat tgaagcggcc
     7081 acgcgcgccg ccgcctcgcc gtcgcggtcg agcacggcga cctccgcgcc ctgcttggcg
     7141 aacgctttgg ccgtcgccgc accgatgccc gaaccgcctc ccgtaatgac cgccacctgg
     7201 cgggccagag atttctcggc gcctttgccg agcttggcct gttcgagcga ccaatattcg
     7261 acatcgaact ggtcttcctc cgaaatcggc cggtaagagc tgccgcgttc ggcggcttcg
     7321 atcacgtcca ccgtattctc ggcgatgtcc gccgcaatgg ccgcatcctt cgccgacgca
     7381 cccagcccaa acaatccgag accggggacc agcaccacgc gcgggaacgg atcgagctct
     7441 ttcttcacac cgcgcagccg cgcattgttg cgcgcgaaat agtcgtgata atgcgccacg
     7501 aacccatcca cggcgctgcg gacggcgtcc gcccatcgat cgagtttccc cgcctcgggt
     7561 gcgggcacga tcagcggcaa attcttggtg cggatggtgt gatcgggtgt ggccacaccg
     7621 atctggctgt agcgcgccag ctccgcgccg ttgacgtaat cgcggatttt ctcgttggtg
     7681 cggaaggcca ggatctggcg ctttgcagtg ccatcctctc gtttgttctc gagcgcgacg
     7741 ctcccgcgca gaatcggcgc gatctcggca acgcccgcaa gccgtcccgg caattgcacc
     7801 ggaacaagag tcttcttgcc gcgcgcgatg tgactttccg cgcgtgagac catctcgatc
     7861 atcagttcat aggcggtgcg cgcgtcttgc gcgaaggtga aaatcccatg ccgcaaaagg
     7921 atcaggccct gaacgtccgg ggtggccgcg taaacgccgg caacgctttt cgccagcgcg
     7981 aagcccggaa tcgtgtacgg cacataggcc atgcgcgccc cgaacgtctc gcgcgcgatc
     8041 tcctcacccg cgggttgatc ggtgagcgcg agcaccgcgg tcgaatgggt gtgatcgacg
     8101 aatttgtgcg gcaggaacgc gtgcagcagc gtttccaccg atgggttcgg cgccgccgaa
     8161 tccaaaagat ggatacgttg ataattcacc atgtcttcgt cggagagttt ttcaagcgcg
     8221 agcagacgcc gcagaggcgc aagcttcacc gccggcaatc cggcaggctc gatatcgccc
     8281 atgtcccagc ccgaaccctt gacgcagatc acctcgatcg catcgccgag caggtctttg
     8341 acctcagtct tgaccgatgt gttgccgccg ccgtgcagca ccagcgcagg atcgccgccg
     8401 aggagacgcg tggtgtaaac gcgcagcgcc aaatcgggag cgatgcccgc atcgcgatag
     8461 cgggcgatga acgcttgggc atcggaatcg gaccagcgcg atttcatgaa gcaatcctac
     8521 ggcatgaggc ctatggcgtt gggccccgca taaagcgggc aaatgcaaaa agcgcaaggg
     8581 gtcgtcacgg acgcaagcct aaaattagcg gccgaactcc ggcgccgggg gcgacgtgcc
     8641 ggtgatttcg gcgtttcgcg ccgcgcgcgc tttggcccgt tcctcggcat aagcatcggg
     8701 cgaggtcttc gccaattgat ccagctcgcc cacatccacg gaccgcttgt cgacctcgtg
     8761 cagcccggtg tggccgacag aggtgatgat gccgcccggc agcgtgacga agtgcaacgg
     8821 gtccatgatg aaatccacca ccttgccgcc cagatcgcgc ggattgctcg gcccgatcac
     8881 cggaatcagc agatagggcg aatcgctgac gccatatttc gccagcgttg cgccgaaatc
     8941 atcgtcgcga taggcgatcc cggcatcgcc ggcaaaatcg aagatgccac cgatgccgat
     9001 ggtggtgttc accacaaaac gcgacagcgt gttgccggcg cgatcccact cacccttcaa
     9061 gagattgttg gccagcacgg tcggcgtctc gagattggtc aggaaattgt ggaggccgtc
     9121 acgcgcggga agcggaacga catagatata gctccgctcg accggatcga taaggtagcg
     9181 atgcaagccg acatggctct tgaagcggcc tctgttggct tcctcgtaag gatcggcgat
     9241 ccggtcggcg gtgatggtga tcctgtccgg ctccggttgc gggaggtact ggtcctcctc
     9301 cgtcattgcg ggcagggccg cgccattgct gagatcgatg tgttgggtac ggggcgtgcg
     9361 gaacgggccg ggtgtgccgg gcgccgtcat ggcaattggc gcgccgccct tgctgaggtc
     9421 gacgaatgtt gtgacggatg atggccgcgc ggtcgcggcg gcttgtggcg atgcttgagc
     9481 gctgccgggt gccgaaagat cgacgaagat tgttttgctg ggatcgcttg gcggggtctc
     9541 ggcgggcgcg gcattcagcg caggcgcgcc ggcagccgat gcgacagaga cgcaagcgca
     9601 tagcctcaag atgcgaagag gaccccaaac gcgcaaagac aatgacatgg attccccgaa
     9661 gcatgacgat gcgagacggg cacgcgcccg gcggacggat tctttagcac aggaagagcc
     9721 ggcctgtgca ttccttaaag ggtgtcacga tttcctcaca cgccgtcggg aaacagcgcg
     9781 cgcagccccg cttcactggc cacacagacg ccacgctcgg tgatgaagcc ggtgacgagg
     9841 cgcgcgggcg tcacgtcgaa accgggattg gcggcgggcg atccgcgcgg tgtgatctcc
     9901 acactcgcga tgctgccgtc cgcaagacgg cccgtcacct gcgtcacttc cgtagcgacg
     9961 cgttcctcga tgggcatgag gccgccggag gccagcgtcc agtcgatggt ggtcgaaggc
    10021 agcgcgacat agaaaggcag atgattgtcc ttcgccgcga gcgccttgag ataggtgccg
    10081 atcttgttcg cgacatcgcc gttccgcgtc acccggtcgg tgccgacaat ggcgagatcg
    10141 acgcggcccg cctgcatcaa atggccgccg gcattgtcgg cgatcaccgc atgcggcacg
    10201 ccatgggacg aaagttcgaa tgccgtcagc gacgcgccct gattgcgcgg gcgcgtctca
    10261 tccacccaga catggaccgg aatgccttcg tcatgggcc
//
LOCUS       DASYEG010000178        12941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2519927, whole genome shotgun sequence.
ACCESSION   DASYEG010000178 DASYEG010000000
VERSION     DASYEG010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 12941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12941
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2519927"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(339..794)
                     /locus_tag="VGM72_08035"
     CDS             complement(339..794)
                     /locus_tag="VGM72_08035"
                     /inference="COORDINATES: protein motif:HMM:NF024030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3775 domain-containing protein"
                     /protein_id="HEY4265251.1"
                     /translation="MNQIITPNTELPPGVELAINPDKVCHIIAKARAFDAKEDISDPD
                     SGSNASDDGMADVLEDMPDDIDATRLELLEFIRALDEEEQINLVALAWLGRGTYDIAE
                     WREALSVARNEHNKRTAEYLLKLPLLGDYLDEGLAAFGENCDSFDEGRM"
     gene            complement(907..2034)
                     /locus_tag="VGM72_08040"
     CDS             complement(907..2034)
                     /locus_tag="VGM72_08040"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEY4265252.1"
                     /translation="MLMLDTHLDTPMNFARPGWDIMDAHSVEGDMSQVDYPRMVKGGL
                     DGGFFAIYTPQGPRTPQGLAAARDAGLKRGVEIREMVARHSDSFALALRADDAQRIAA
                     SGKRVVFMSMENGYPLGKDLTLLKTFYDLGVRMAGPVHFTNNDLADSATDPKGPEWHG
                     LSPLGKQFVAQANRLGIILDASHASDDVFDQMLALSAAPIILSHSGVRAVFNHPRNID
                     DTRIARLAAAGGVIQVNSYSDYLVDTPVIPARETAMRALGVKYGPFRALAGDKLKAYM
                     AERHAVEAQYPLPHATMDDLMAQLLHALKLAGPDHVGIGLDWDGGGGVTGMEDVAGIP
                     AISKGLLAAGYSQADLAKIWGGNALRVMRAVEAKAAELARK"
     gene            2256..3017
                     /locus_tag="VGM72_08045"
     CDS             2256..3017
                     /locus_tag="VGM72_08045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4265253.1"
                     /translation="MNRRKRALVTGGATGIGGAAVLHLARAGFDVALNYRSSAEAARA
                     VAAEAEAAGAKILLLPGDVAEDSEVRKLMARVDETFGRLDALINNAGTTVSTPPKDLE
                     GLSVEDWDRVFAVNVRGLFLVTRAAVPLLKRGENPAIVNTASIVGLRPGPQPLPYAAS
                     KAAIVSLTKQFAGALGPEIRVNAVAPGWMDGEWMERMLGGNYDRLMERRAKATPLGRC
                     VTADDVAEVMLSLIQSNRFVTGEIVVIDGGFSSTT"
     gene            3017..4675
                     /locus_tag="VGM72_08050"
     CDS             3017..4675
                     /locus_tag="VGM72_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY4265254.1"
                     /translation="MLHGFVPFPPEFAARYRAKGYWEDRTLAQVWEERCRTFADRIAV
                     IDGDVVATYAGIDARATNLALNLLGLGIRPGDRLVVQLPNRIQFVYLYLALQKIGAIP
                     VMALPAHRYLEISQFVQIAGAIGCVMPDRHRDFDFRALVTRIRKEQPQLTHGIILGDA
                     PPGFASLDELIARKSPRDPGELGRIAIDPAQPALFLLSGGTTGVPKLIPRTHNDYVYN
                     SKMAGSVTGIADDSVLLDVLPISHNLPLACPGLQGFLFRGAKVVLSTDIAPEAIFRLV
                     EKHRVTHIHVVPALLIRWINDPAIARYDLSSLRVIQSGGQRLQPETRAKTHELIPSVT
                     VQENFGMAEGVLMFVRLDDPPEVRLETVGRPVCPDDEIMILDEDDNVVPFGEIGEFCC
                     RGPYTLRGYYGAPDHNARAFTRDGFYRSGDLMRQHVSGNYMVEGRKKDLVNRGGEKIS
                     AEEIENLILSHPAVQNVACVPVPCPELGERMCACLVLQPGCTLTFDALIGFLETKEMA
                     KIKLPERLLVLDALPLSNFGKVSKKTLSEMAAKHAIARKSGRRG"
     gene            4813..5652
                     /locus_tag="VGM72_08055"
     CDS             4813..5652
                     /locus_tag="VGM72_08055"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4265255.1"
                     /translation="MRKIDLHAYPGTQEWIACQGPYVDALAAYWKREWKPKSEADVVA
                     EFEAAGVDVCLVALDLSATIGTPPCSNDYTAGMRDRHRGIVQAWAAVEPAKGRPAIDE
                     ARHAVRDLKMMGFHFHPIMQHFAVDDPRHYPLFEEIAALNVPVMIDVGMTGMGAGMPG
                     GMGAKTRHAHPSSIDALAADFPNLTIIMAHPGYPWIDETTAVALHKGNVYWEMSGWAP
                     KYLPPAIIRDMRGRLADKMMFGSDYPSIPYARLFKEWEELGFSDTFLEKFYHGNAERI
                     FGL"
     gene            5663..6427
                     /locus_tag="VGM72_08060"
     CDS             5663..6427
                     /locus_tag="VGM72_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY4265256.1"
                     /translation="MNEPLDVVMDTAGIAQVTINRPKRANAVDPALAKAMLEAFDWLA
                     NDPKLRVLVLLGNGRTFCAGLDLKAMTDTASSAFEENYQDALLLTKLLHRLDTMPVPT
                     LAVVPGAAVGLGVGLVACCDVVLASEVASFRFSEVRLGIIPAVISPYVIAAMGPRACR
                     RYFLSGESFDATEAMRLGLVHAVSNLDELGAKAAQLASELHAGKRGAQAAAKTLIEDI
                     RLAQVDEALIQETARRLAEIRSKPEAQAALAEFLKA"
     gene            6591..7199
                     /locus_tag="VGM72_08065"
     CDS             6591..7199
                     /locus_tag="VGM72_08065"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY4265257.1"
                     /translation="MAEGDTTSTAERPKGAAGPGHNSGTEEPVVWERWTKKRTFVRAL
                     EGTYGELVHELFSQPRVYHSSDLKWKGGPQNFGKKVINPGSAKVAQSIECHIDCFAPG
                     GYGQRHGHMNSAVFYVLKGKGHDVHDRRRLDWEAGDALIVENGCVHQHLNDDPNEEAI
                     VLVAKAKPLFLFMHLIFQKVAAWPPKEAESQAQRDYLPPTDI"
     gene            7217..7774
                     /locus_tag="VGM72_08070"
     CDS             7217..7774
                     /locus_tag="VGM72_08070"
                     /inference="COORDINATES: protein motif:HMM:NF014136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265258.1"
                     /translation="MSQSIDPRERDKSHAEVEQVDFYAQALKVSEEFRGEYQDRLNVV
                     KASQMPMERSRQGLIKHIINERMNTKECCLDMYQQFLPPGKASGKHRHLSEEVFYVIE
                     GNGYDLHWDVRFDCDHEMDFSWDEEPKRFEWGQGDFVYIPPYCAHQHFNADPNKEARI
                     VVINSRIVKAMGFDWYEQIEDAEGY"
     gene            7824..8822
                     /locus_tag="VGM72_08075"
     CDS             7824..8822
                     /locus_tag="VGM72_08075"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY4265259.1"
                     /translation="MLKRLLAATMGAALLWSASAMAAADMGQFRGKTITYIVSTSPGG
                     GYDTYGRLIAKYLQKYLTGSRVVVRNVPGAGHIVGANTIYAAKPDGLTIGMFNTGLIY
                     DQLIKRQGVLFDLAKFSWIGKAADDTRVLMVSKSAPYKTIGDILKVKTPIKFAAAGVG
                     SAAYNDARILADALHLNVQIVPGFTGNEGEMSMLRNEVVAEVGTAESMEQFVKQGHGF
                     FALALSGNPNALPGVPRARDYVKDERGKRLLTLIETLSEIGRLTAGPPGMPAGVLAAE
                     REALDAAMKDPALIAEAKKLDLDLDPASGALVEARIKAALNQPPETVAALKSAAGG"
     gene            8868..10412
                     /locus_tag="VGM72_08080"
     CDS             8868..10412
                     /locus_tag="VGM72_08080"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY4265260.1"
                     /translation="MHLDVAFSTLFDLLTTPRLLFLMVIAVPMGVFFGSTPGLGGKLG
                     LVLLIPFVYNMDQLTGAVFLCAMHAVVHTGGSIPSILIGVPGDGPAAATALDGFAMTR
                     KGQAGRALGASFGASTVGGLLGAMFLGLSLPILEPIILNFSPAEFFLLAILGITFIAT
                     LSGDSFIKGMIVGLFGLMCAFIGLDPSTGSPRFTFGQIWLWDGLSVIVAVLSMFAVPE
                     MISLGVRGRSEDVSAQTASRLQGTAAVEETVSRKVSYAQIFHGLWDVTQHKWLVLRCS
                     LLVAAIGVIPGLGGDAAAWMAYGHTVQSSKHPERFGKGEVAGVIGAETGSCAKEAGSL
                     LPTLFFGVPGSSGMAIMLGAFLMLGIQPGPTMLTDHINIVWSLIWALVIANIICVIML
                     IATGPWIGRLGSVKASFLIPYVLVFALLGCYLYGGNWQNLILVMVVGSLGYLFKRHDW
                     PRAPFIIGVILGKIAEDSLQKAIAIFGAGFFLRPISLILIALILASIGFYMWRARRPK
                     IVPSHA"
     gene            10421..10861
                     /locus_tag="VGM72_08085"
     CDS             10421..10861
                     /locus_tag="VGM72_08085"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEY4265261.1"
                     /translation="MLEGRLLVSGIMLVVFAAAVGLSFTYAPEARFLPLVIGIPGLLL
                     SAIQFVAELRDRHPAPVVTREEHHREGRMFAWFLGFVGALVLFGFLYAGPALVAAYLY
                     FSGREKWYVALAGAAFAWTVLYGVFDWFLGLPLFDGLVFQYVFG"
     gene            10905..11306
                     /locus_tag="VGM72_08090"
     CDS             10905..11306
                     /locus_tag="VGM72_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265262.1"
                     /translation="MCGELEEEGSAADEVYGRFVAHANAFLAGMKRERRRPDPIHNHL
                     DRLFRELLSAAADENRAPDEMSGYQRIAMEPLVFARLAGFIAAHVPLNEDPLRRVMEA
                     LMLGYSEGEIALPDHDHDHDPFHGHDHGHSH"
     gene            11319..>12941
                     /locus_tag="VGM72_08095"
     CDS             11319..>12941
                     /locus_tag="VGM72_08095"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY4265263.1"
                     /translation="MTDAIANAMPNRGWQSDVIADLIKGFGFPYITLNPGASYRGLHD
                     SLVNHNRNEPPMLLCQHEKIAVQIAHGYAKACGEPLCVIVHNVVGLLHATMGIYYAYT
                     DRAPVFVVGATGPMDEGKRRPRVDWDHTANVQGNAVRDYVKWDYQPGGIQGVPDSFAR
                     AYSIMMSEPQGPIYLCYDAGLQEAPLTETVKLPDPASVKRPSKLAPDLKALAEIADRL
                     VAAEWPVLLPQYVGRSPSGFTDMMALAETMGAGVVDVQSRLNFPTEHPLDCRMSTDIF
                     KRADLILSLDCRDWERPTHYNDRINRTLKAHYPDSCVWADIGFADIEISKWAMGYQKF
                     PQCAARVLADTELAIPQLTELCAARVAKDAKAARRIADRARAVGEMHNTQRAKWRGQA
                     REGWDASPIALPRLAMEVWEQIKNEDWVLTTNAFEDWALKLWDFDKHYRWPGKALGTG
                     TQIGMAIGVALAHRGKGRLVVDCQTDGDLMFDAGALWFAAKHQVPLLCVMHNNRAYYN
                     DWEHQITVARQRGTPEERAYIGMDIMEPAPDFA"
BASE COUNT         2323 a         4041 c         4035 g         2542 t
ORIGIN      
        1 gcctctgccc cgcgatggcg ggcggaatct ttcaagagct cggtgatctt ggaaaaatcg
       61 gcgcgcaaat tccgcaattc ggcgcgaagc gcctgaacgt cgccgtctgt gtctgagccc
      121 gcaacatagt cgttcatgat agtcccctca gatcgaactg gcctctcaga tcaaactggt
      181 ctctcagatc gaaccggcct ctgagatcga accagcctcc gcctgatatg gcgctacctg
      241 ccaatttccg tcaacacgct aacctctccc tctccgctgt ccaagccatg gggaataaaa
      301 aagcccgaaa acgccggggc tcgcggtgtt tcgctttatt acatccgtcc ttcgtcgaac
      361 gaatcgcagt tctcgccaaa ggccgccagg ccctcgtcca gataatcgcc gagcagggga
      421 agcttcaaaa gatattccgc ggtgcgcttg ttgtgctcgt tgcgggcgac actgagcgct
      481 tctcgccatt ccgcgatgtc ataagttcct cggccgagcc aagccagcgc caccagattg
      541 atctgttctt cttcgtccag ggcgcgaatg aattccagga gctcaaggcg ggtggcgtcg
      601 atgtcgtccg gcatatcctc cagcacgtcg gccatcccat catccgaggc attcgatccg
      661 gaatcgggat cgctgatgtc ttccttcgcg tcgaaggcgc gcgccttggc gatgatgtgg
      721 cacaccttgt cgggattgat ggcgagttcc acgcccgggg gcaactcggt gttcggcgtg
      781 atgatctggt tcatggcctc tctcgccgcc cagcggcgcc gttttcgctt cgccttggtt
      841 acgctccgtg cgggataagg ttcacgcgcg ggaaccccaa aaccgctccg gcgaccataa
      901 atggcgttat ttgcgggcca gttcggcggc cttggcctcc accgcgcgca tgacgcgaag
      961 cgcgttgccg ccccagatct tggcgagatc ggcttgcgaa tatcccgcgg ccaggaggcc
     1021 tttgctgatc gccggaatac cggccacgtc ctccatgccg gtgaccccgc cgccgccgtc
     1081 ccaatccagc ccgatgccga catgatcggg gccggccagt ttcagagcgt ggagcagttg
     1141 cgccatcaga tcgtccatcg tggcgtgggg gaggggatat tgcgcttcca ccgcgtggcg
     1201 ctcagccatg taggctttca gtttgtcgcc cgcgagcgcg cggaacgggc catatttgac
     1261 gcccagcgcc cgcatcgcgg tttcccgcgc cgggatgacc ggggtgtcca ccagataatc
     1321 gctgtaggaa ttgacttgga tcacaccgcc cgccgccgcg agcctggcaa tgcgcgtatc
     1381 gtcgatattg cgcggatggt tgaacacggc ccgtacgccg gaatgcgaaa ggatgatcgg
     1441 ggccgccgac aatgccagca tctggtcgaa cacgtcgtcg gaggcatggg aggcgtccag
     1501 gatgatgccg agccggttgg cctgcgccac gaactgtttt cccaagggcg acagcccatg
     1561 ccattccggc cccttggggt cggtcgcgga atcggcgagg tcgttgttgg tgaaatgcac
     1621 cggcccggcc atgcgcacgc cgagatcgta gaaggtcttc agcagcgtca ggtccttgcc
     1681 cagaggatag ccgttctcca tgctcatgaa gacgacgcgc ttgcccgagg ccgcgatgcg
     1741 ctgagcgtcg tcggcgcgca aggccaacgc gaagctgtcg gaatggcgcg ccaccatctc
     1801 gcggatttcg acgcctcgtt tcagccccgc atcgcgcgcc gccgcaagcc cttgcggcgt
     1861 gcgcggcccc tggggcgtgt agatcgcgaa gaagccgcca tcgagtccgc ctttgaccat
     1921 gcgcggataa tcgacctggg acatatcgcc ttcgacgctg tgcgcatcca tgatgtccca
     1981 gccgggccgc gcgaaattca tcggcgtatc cagatgggtg tcgagcatga gcatgtgcgc
     2041 gggcgcgtcc gccgcccatg ccgccggcgt taggccgagg aaaagtgccg cccatgctgc
     2101 gcggcaaatt gctctgcgcc aaattgcttt gtgactcgcc atcgtccgcc tcgaaaactt
     2161 gcaaccactg gcgcgagctt agcggatttg cagtcgcggc gtcattcgcc tagatcagac
     2221 gcttcggtgg agcgtgtttg ggaagggaca gcggaatgaa ccggcgcaag cgcgcgctcg
     2281 tcaccggcgg ggccaccgga attggcgggg ccgcggtgct gcatctggcg cgggcgggat
     2341 tcgatgtcgc gctgaattat cgctcgagcg cggaggcggc gcgtgcggtt gccgcggagg
     2401 ccgaggcggc aggcgcgaaa atattgctgc tgccgggcga tgtggccgag gacagtgagg
     2461 tccgcaagct gatggcgcgc gtcgatgaga cgttcggtag gctggacgcg ctcatcaaca
     2521 atgcgggcac cacggtctcc acgccgccaa aagacctcga aggattaagc gttgaggatt
     2581 gggatcgcgt ctttgccgtg aatgtacgcg ggctctttct cgtgacgcgt gcggcggtac
     2641 cgcttctgaa gcggggtgaa aaccccgcca tcgtgaacac cgccagcatt gtgggcttgc
     2701 gtcccggtcc gcagccgctg ccctatgcgg cgagcaaagc ggccatcgtc tcgctgacca
     2761 agcaattcgc cggcgcgctc ggccccgaaa tccgcgtcaa tgcggtggcg cccggctgga
     2821 tggatggcga atggatggaa cgcatgttgg gcggcaatta cgaccggctg atggaacgcc
     2881 gcgccaaggc gacgcctttg gggcgctgcg tcaccgccga cgacgtggcg gaagtcatgc
     2941 tgtcgctgat ccaatccaac cgcttcgtga ccggcgagat cgtcgtcatc gacggcggat
     3001 tttccagcac cacgtgatgc tgcacgggtt tgttccgttc cctcccgaat tcgcggcccg
     3061 ctaccgcgcc aaaggctatt gggaagaccg cacgctggcg caagtgtggg aggagcgttg
     3121 ccgcacattc gcggatcgca ttgccgtcat cgatggcgat gtcgttgcca cctacgccgg
     3181 catcgacgcg cgcgcgacca atctcgcgct caatctgctc ggcctcggca ttcgccctgg
     3241 tgaccggctg gtcgtgcagc tcccgaaccg tatccaattc gtctatctgt atctcgcgct
     3301 gcagaagatc ggggccattc cggtgatggc gcttcccgcg catcgctatc ttgaaatcag
     3361 ccagttcgtg cagatcgcag gcgccattgg ctgcgtcatg cccgaccgcc atcgcgattt
     3421 cgactttcgc gcccttgtca cccgcatccg caaggagcag ccgcagctca cgcacggcat
     3481 catcctgggc gacgcaccgc cgggatttgc ttcgctggat gaactgatcg cgcgaaaatc
     3541 cccccgcgat cccggggagc ttggccggat cgcaatcgat cccgcgcagc ctgcgctgtt
     3601 tctcctctcg ggtggcacca cgggcgtgcc gaaactgatc ccgcgcacgc ataatgatta
     3661 tgtctacaat tccaagatgg ccgggtcggt gacggggatt gccgatgatt ccgtgctgct
     3721 cgacgtgctg ccgatctcgc acaatctgcc gctcgcctgt cccgggcttc agggtttcct
     3781 gttcaggggc gcgaaagtcg tgctttccac ggacatcgcg cccgaggcga ttttccgtct
     3841 ggtcgagaaa catcgcgtca cgcatatcca tgtcgtgccg gcacttctca tccgctggat
     3901 caacgatccg gccattgcgc gttacgacct gtcgtctttg cgcgtcatcc agagcggcgg
     3961 tcagcggctt cagccggaaa cgcgcgcgaa gacccacgaa ctaattccga gcgtcaccgt
     4021 tcaggagaat ttcggcatgg ccgaaggcgt cttgatgttc gtccggctgg acgatccccc
     4081 cgaggtgcgg ctcgaaaccg tcggccggcc tgtttgtcca gacgacgaga tcatgatcct
     4141 ggatgaagac gacaatgtcg ttccgttcgg cgagatcgga gaattctgct gccgcggtcc
     4201 ctatacgctg cgtggctatt atggcgcccc ggaccacaat gcccgcgcct tcacgcggga
     4261 cgggttttac cgctcgggcg atctgatgcg tcagcacgtt agcggcaatt acatggtgga
     4321 gggccgcaag aaggatctcg tcaaccgggg tggcgaaaag atcagcgcgg aggaaatcga
     4381 gaacctcatt ctttcccatc ccgccgtgca gaatgtcgcc tgcgtgcccg tcccgtgccc
     4441 cgagcttggg gagcgcatgt gcgcctgcct cgtgcttcag ccaggctgta cgctgacatt
     4501 tgacgcgctc atcggattcc tggaaaccaa ggagatggcg aaaatcaaac tccccgagcg
     4561 gctgctggtg ctcgacgcct tgccgctttc caatttcggc aaggtgtcga agaagacgct
     4621 ttccgaaatg gccgcgaaac atgccatcgc gcgcaagtcc ggacggcgcg gatgagaaaa
     4681 accactaaac gcaccaagaa cgcacgaagg gcacaaagat cgcttcgtgt cctttgtgca
     4741 tcctttgtgt tcttcgtgtc tctctttgac tttatatccc gacgctatgt ttttgcgtga
     4801 atgactaatc cgatgcggaa aatcgacctc cacgcctatc ccggcacgca ggaatggatc
     4861 gcctgtcagg ggccctatgt ggacgcgctt gccgcctatt ggaagcgcga atggaaaccg
     4921 aaatccgaag ccgacgtggt cgccgaattc gaggccgccg gtgtcgatgt gtgcctggtg
     4981 gcgctcgatc tttcggccac catcggcacg ccgccctgct cgaacgatta caccgcgggg
     5041 atgcgcgacc gccaccgcgg catcgtgcag gcctgggccg cggttgaacc ggccaaaggc
     5101 cggccggcca tcgacgaggc gcgccatgcg gtccgcgacc tgaagatgat ggggttccac
     5161 ttccatccga tcatgcagca tttcgcggtg gacgatccgc gccattatcc gctgttcgag
     5221 gaaattgcgg cgctgaacgt acccgtgatg atcgatgtcg ggatgacggg catgggcgcg
     5281 ggaatgcccg gcggaatggg cgcgaagaca cgccacgcgc atccgtcatc catcgacgcg
     5341 ctggcggctg actttccgaa tctcaccatc atcatggccc atccgggcta tccctggatc
     5401 gacgagacga cggcggtggc actccacaag ggcaatgtct attgggagat gtcgggctgg
     5461 gcgccgaaat atctaccgcc tgcgatcatc cgcgacatgc gcgggcgctt ggccgacaag
     5521 atgatgttcg gcagcgacta tccgagcatt ccttatgcgc ggctgttcaa ggaatgggag
     5581 gaactcggct tctccgatac gttcctggag aaattctacc acggcaatgc ggagcggatt
     5641 ttcggattgt gaggcgccaa agatgaacga accactcgac gtcgtgatgg atacggccgg
     5701 catcgcgcag gtcacgatca atcgccccaa acgcgccaat gccgtcgatc cggcgctggc
     5761 aaaggcgatg ctcgaagcgt tcgactggct ggcgaacgat cccaagctgc gggtgctggt
     5821 gctcttgggc aatggccgca ccttctgcgc cgggctcgac ctgaaggcga tgaccgatac
     5881 agcgtcgtcc gcgttcgagg aaaattatca ggacgcgctg ctgttaacca agctgctgca
     5941 tcggctcgac accatgccgg tgccgacgct cgcggtcgtg ccgggtgccg ccgtcgggct
     6001 cggcgtcggg cttgtcgcct gttgcgatgt tgtcctcgcc tcggaagtgg cgagctttcg
     6061 cttttccgaa gtgcggctcg gcatcattcc cgccgtcatc tcgccctatg tcatcgcggc
     6121 gatggggccg cgcgcgtgcc ggcgttattt cctttcaggg gaaagtttcg atgcgaccga
     6181 agctatgcgg ctcggacttg tccatgcggt ttccaatctg gatgaactgg gcgccaaggc
     6241 ggcacagctc gcctcggagt tgcacgccgg caagcgcggc gcacaggcgg cggccaagac
     6301 cctgatcgag gacatccgtc ttgcacaggt cgacgaagcc ctgatccagg aaaccgcccg
     6361 ccggctggcg gaaatccgca gcaagccgga agcgcaagcc gctctcgccg aattcctcaa
     6421 agcttgacct catcactgag gattgaccgg cgctttttca tctttgcgcc atgctcaggg
     6481 ccgcggactt gacagccata ggagcctatg ggacggctct acgaacctcg gttggatgcc
     6541 catgggcgtc aaaaccggct cataacatgt gacatttcag gaggtcatcc ttggcggaag
     6601 gcgatacgac aagcacggcg gaacgcccga aaggcgcagc cggacccggc cacaacagcg
     6661 gcaccgagga acccgtcgtc tgggagcgct ggacgaagaa acgcaccttc gtgcgggcgc
     6721 tcgaaggcac ctatggcgaa ctcgtgcatg agctgttctc gcagccgcgc gtctatcatt
     6781 cctcggattt gaagtggaag ggcggaccgc agaatttcgg caagaaggtc atcaatccgg
     6841 gctccgccaa ggtcgcccag tcgatcgagt gtcacatcga ctgcttcgct ccgggcggct
     6901 acggccaacg ccatggccat atgaacagcg ccgtcttcta tgtgctcaag ggcaaaggcc
     6961 acgatgtcca tgatcgccgc cgcctcgatt gggaagcggg cgatgcgctg atcgtcgaga
     7021 atggctgtgt gcaccagcat ctgaacgacg atcccaatga ggaagccatc gtgctggtcg
     7081 ccaaggccaa gcccctcttt cttttcatgc atctgatctt ccagaaggtc gccgcctggc
     7141 cgccgaaaga ggccgagagc caggcccagc gcgactatct tccgccgacc gacatctgaa
     7201 aaaggttttg agacgaatga gccagtccat cgatccgcgc gaacgcgata agtcccatgc
     7261 cgaagtcgag caggtggatt tttatgcgca ggcgctgaaa gtttccgaag aattccgtgg
     7321 cgaatatcag gatcgcctga acgtggtgaa ggcgagccag atgcccatgg agcgttcgcg
     7381 ccaggggctc atcaagcaca tcatcaacga gcggatgaac accaaggaat gctgcctgga
     7441 catgtatcag cagttcctgc cgcccggcaa agccagcggc aagcaccgcc atctgtcgga
     7501 ggaagtgttc tacgtcatcg aaggcaatgg ctacgatctg cattgggatg tgcgcttcga
     7561 ctgcgaccat gagatggact tcagttggga cgaagaaccc aagcgcttcg aatggggcca
     7621 gggcgatttc gtctacatcc cgccctattg cgcgcatcag catttcaacg ccgatcccaa
     7681 caaggaggcg cgcatcgtcg tcatcaacag ccgcatcgtg aaggcgatgg gtttcgattg
     7741 gtacgagcag atcgaggacg ccgaaggcta ttgagccttg tgagtgcgcc gggaccgcgt
     7801 ccgctgttgc aggaggagat cgcatgctga agcgtttgct ggcggccacg atgggagccg
     7861 ctcttctctg gtccgcttcc gcaatggctg ccgccgacat gggccaattc cgcggcaaga
     7921 ccatcaccta catcgtctcg acgagtccgg gcggcggcta cgacacctat ggccgcctga
     7981 tcgcgaaata tttgcagaaa tatctgacgg ggtcccgtgt ggtggtgcgc aacgtgccgg
     8041 gcgcgggcca catcgtgggg gccaatacca tctatgccgc caagcccgac gggctgacca
     8101 tcggcatgtt caacaccggc ctgatctacg atcaattgat caagcgccag ggtgtgctgt
     8161 tcgatctggc gaaattcagt tggatcggca aagcggccga cgacacccgc gtgctcatgg
     8221 tctccaaaag cgcgccgtac aagaccatcg gcgacatcct caaggtgaag acgccgatca
     8281 aattcgccgc cgcgggcgtc ggctccgccg cctataatga cgcgcgcatt ctggccgacg
     8341 cgctgcatct caacgtccag atcgttccgg gctttaccgg caacgagggc gagatgagca
     8401 tgctgcgcaa cgaagtcgtg gccgaagtcg gcaccgccga atccatggag caattcgtca
     8461 aacaaggtca cggtttcttc gcgctggcgc tttcgggcaa tccgaacgcg cttcctggcg
     8521 tgccgcgcgc ccgcgattac gtgaaggacg agcgcggcaa gagattgctc acgctcatcg
     8581 agacgctgtc cgaaatcggc cgcctgacgg ccggtccgcc gggcatgccg gctggtgtcc
     8641 tggccgccga gcgcgaggcg ttggacgccg cgatgaagga ccccgccctt atcgccgaag
     8701 ccaagaagct cgatctcgat ctggaccccg cttcgggcgc gttggtcgag gcccggatca
     8761 aggccgccct gaaccagccg cccgagacgg tcgccgcgct caaatccgcg gccggcgggt
     8821 gatggcggga cgctgacgcc ggcgaacgcg gaaatgagga cgcgctgatg catctcgatg
     8881 tcgccttctc gacgctgttc gatctgctga ctacgccgcg tcttttgttt ctgatggtca
     8941 tcgccgtgcc catgggggtc tttttcggct cgacgccagg gctcggcggc aagcttggtc
     9001 tcgtcctgct gatcccgttt gtctacaaca tggatcaatt gacgggcgcg gtgttccttt
     9061 gcgccatgca cgcggtggtc cacaccggcg gctccattcc cagcatcctg atcggggtcc
     9121 ctggcgatgg accggcggcc gcaaccgcgc tcgacggctt tgcgatgacc agaaagggac
     9181 aggcgggacg cgcgctcggc gcgagcttcg gcgcctccac cgtcggcggg ctcctgggcg
     9241 cgatgttcct gggattgtcc ttgccgatcc tggaacccat cattctcaat ttcagcccgg
     9301 ccgagttctt ccttctcgcc attctcggca tcaccttcat cgccacgctc tcgggcgaca
     9361 gtttcatcaa gggcatgatc gtcgggctct tcggtttgat gtgcgcgttc atcgggcttg
     9421 atccttcgac gggaagcccg cgtttcacct tcgggcagat ctggctgtgg gacggccttt
     9481 ccgtgattgt cgccgtgctc tcgatgttcg ccgttcccga gatgatctcg ctgggtgtcc
     9541 ggggccgctc cgaggatgtt tccgcgcaga ccgcgtcccg gctgcagggt accgcagccg
     9601 tcgaggagac cgtgtcgcgc aaggtgagct atgcgcagat atttcatggg ctttgggatg
     9661 tcacccaaca caaatggctg gtcttgcgct gttcgctgct ggtggcggcc atcggcgtca
     9721 ttccgggcct cggcggcgat gccgcggcct ggatggccta tggtcatacg gttcagtcct
     9781 ccaagcatcc cgaacgtttc ggcaagggcg aggtcgcggg cgtgatcggc gcggagaccg
     9841 gaagctgcgc caaggaggcg ggctcgctgc tgccgaccct gttcttcggt gtgcccggtt
     9901 cgtcgggcat ggcgatcatg ctgggggctt tcctgatgct cggcatccag cccggtccca
     9961 ccatgctgac cgatcatatc aacatcgtct ggtcgctgat ctgggcgctc gtcatcgcca
    10021 acatcatttg cgtcatcatg ctgatcgcga ccggtccgtg gatcggcagg ctcggctcgg
    10081 tgaaggcgag ctttctcatt ccctatgtcc tggtgttcgc gctcctgggt tgttacctct
    10141 atggcggcaa ttggcagaac ctcattctgg tcatggtggt gggcagcctc ggctatttgt
    10201 tcaagcgcca tgactggccc cgggctccct tcatcatcgg cgtcattctc ggcaagatcg
    10261 cggaggattc gcttcagaag gcgatcgcga tcttcggcgc gggtttcttc ctgcggccga
    10321 tttcgctcat cctgatcgcg ttgatcctgg ccagcatcgg cttctatatg tggcgcgcgc
    10381 gccggccgaa gatcgtgccc agccatgcgt gaggcctccc atgcttgaag gaagacttct
    10441 ggtcagcggc atcatgctgg tcgtgtttgc ggccgccgtg ggcctgtctt tcacctatgc
    10501 gccggaggcg cgcttcctgc cgctggtgat cggtattccc ggcctgttgc tctcggccat
    10561 tcagttcgtg gccgagctgc gcgacaggca ccccgcgccc gtggtgacgc gcgaggagca
    10621 ccaccgcgaa gggcgcatgt tcgcttggtt tctcggcttt gtgggtgccc tggtgctgtt
    10681 cggctttctc tatgccggcc cggcgctggt ggcggcctat ctttactttt cggggcgtga
    10741 gaaatggtac gtggcgcttg ccggcgcggc gtttgcctgg acggtccttt acggcgtgtt
    10801 tgactggttc ctcggtctgc cgctgttcga cgggctggtc ttccaatatg tgttcggctg
    10861 attacagccg ttgacggcat cgaaatcgac gcgtggagtt gagcatgtgt ggcgaattgg
    10921 aagaagaagg cagcgcggcc gacgaggtgt atggccgctt tgtcgcgcat gcgaacgcgt
    10981 ttctggccgg gatgaagcgc gagcgccgca gacccgatcc gatccataac catctcgacc
    11041 ggctgttccg cgagctgctt tcggcggccg ccgacgagaa tcgcgcgcct gacgaaatga
    11101 gtggctatca gcgcatcgcg atggagcctc tggtgttcgc gcgcctcgcc gggttcatcg
    11161 cggcccatgt gccgctgaac gaagaccccc tgcgccgggt gatggaagcc ttgatgctcg
    11221 gctattccga aggcgagatc gcgcttcccg atcacgacca tgatcatgac ccgttccacg
    11281 gtcacgatca tggccattcg cactgacggc agcgattcat gaccgatgca atcgcgaatg
    11341 ctatgcccaa tcgcggctgg cagtcggacg tcatcgcgga cctcatcaaa gggttcggat
    11401 ttccctacat cacgctcaat cccggcgcct cctatcgcgg gctgcacgat tcgctggtca
    11461 atcacaatcg caacgaaccg ccgatgttgc tgtgtcagca cgagaagatc gcggtccaga
    11521 tcgcgcatgg ctatgccaag gcctgcggcg aaccgctctg cgtcatcgtg cacaatgtgg
    11581 tgggtctgct gcacgccacc atgggcatct attacgccta taccgatcgc gcgccggtct
    11641 tcgtggtggg cgccaccggc ccgatggacg agggcaagcg ccgcccgcgc gtcgattggg
    11701 atcacaccgc caatgtgcag ggcaacgcgg tgcgcgacta tgtcaaatgg gactaccagc
    11761 ccggcggcat ccagggcgtg cccgacagtt ttgcgcgggc ctattcgatc atgatgagcg
    11821 agccgcaagg cccgatctat ctctgctatg acgcagggct tcaggaagcc ccgctcaccg
    11881 aaacggtgaa gctgcccgac cccgcatccg tgaaacgccc cagcaaactc gcgcccgatc
    11941 tgaaggcact cgccgaaatt gcggacaggc tcgtggcggc cgaatggccg gtgctgctgc
    12001 cccaatatgt gggacgcagc ccaagcggct tcaccgacat gatggcgctt gccgaaacaa
    12061 tgggcgccgg tgtcgtggac gtgcaatcgc ggctgaattt tcccaccgag catccgctcg
    12121 attgccggat gagtacggac atcttcaaac gggccgacct gatcctttcg ctcgactgcc
    12181 gcgattggga gcgcccgacc cattacaacg accgcatcaa tcgcacgctc aaggcgcact
    12241 atcccgattc ctgcgtctgg gccgatatcg ggttcgccga tatcgagatt tccaaatggg
    12301 ccatgggcta ccagaaattc ccgcaatgcg cggcccgcgt tctcgccgat accgaacttg
    12361 ccattcctca gctcacggag ctttgcgccg cacgcgtggc aaaagacgcc aaggccgcca
    12421 ggcgcatcgc cgaccgcgcg cgcgcggttg gcgagatgca caacacccag cgcgccaaat
    12481 ggcgcggcca agcgcgcgaa ggctgggacg cgagccccat cgcgctgccg cgcctggcca
    12541 tggaggtgtg ggagcagatc aaaaacgaag actgggtgct caccaccaac gcgttcgagg
    12601 attgggcgct gaagctgtgg gatttcgaca agcactatcg ctggccgggc aaggcgctcg
    12661 gcaccggcac gcagatcggc atggcgatcg gggtcgcgct cgcccatcgc ggcaagggac
    12721 ggctcgtggt cgattgtcag accgacggcg atctgatgtt cgatgcgggc gcgctctggt
    12781 tcgccgcgaa gcaccaggtg ccgctgctct gcgtgatgca caacaaccgc gcctattaca
    12841 atgattggga gcaccagatt acggtggcgc gccagcgcgg cacgccggaa gaacgcgcct
    12901 atatcggcat ggacatcatg gagcccgcgc ccgatttcgc g
//
LOCUS       DASYEG010000179         7488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4838662, whole genome shotgun sequence.
ACCESSION   DASYEG010000179 DASYEG010000000
VERSION     DASYEG010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 7488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7488
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4838662"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2255
                     /locus_tag="VGM72_08100"
     CDS             <1..2255
                     /locus_tag="VGM72_08100"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEY4265264.1"
                     /translation="TMACVVRPMTARRQKLMRQTTLIERVRAYDPGADESILNKAYVF
                     AMQAHGPQRRASGDPYFSHPLEVAGILTDLKLDTATIATALLHDTVEDTSATIKDIES
                     NFGGEIAGLVDGVTKLSQLELFSERTKQAENFRKLMLAMSNDIRVLLVKLADRLHNMR
                     TLSHIKSPEVRHRIAQETIEIYAPLAGRIGMQNMREELEDLAFAELDPEARESIVRRM
                     ERLDAQSSARIQRIADEIKRKLAENGLDAWVLGRAKRPYSIWRKLKDKKSNFEQLSDV
                     FGFRAVVGSVEDCYRALGVLHRSWRMVPERFKDFISLPKSNGYRSIHTTVMGPENQRV
                     EVQIRTQEMHEVAERGVAAHWRYREKINENDPTAMRAYEWLRDMVDLLERGESPEEFY
                     EHSKLSLYQDQVFCFTPKGMLIALPRGATPIDFAYFVHTDVGNTAVGAKVNGVHVPLQ
                     TPLKNGDQVEIIGSREQSPSPLWERFVVTGRARAEIRRYLRHAQRGQHVSLGRKILAK
                     IFADEGFELTDKAVEGVLRKLRLSKAEDVYADVGRGALRADEVLQAVYPELKRDPKRK
                     RASYLEPNSAGVSIGGLTEGIGYRLGSCCHPIPGDRIIGLMVPGEGCVIHTVDCDVLD
                     RHQDSMADWIDVRWKDHGGALSVARIVVRLKNSTGALATLANVISHNGANISNLKITS
                     RNPLYFEFQVDAEVRDAAHLENLIRALKVDSAVEAVERVRSLDTDDAGDGPPPVSETQ
                     GLLPFEHARH"
     gene            2242..3009
                     /locus_tag="VGM72_08105"
     CDS             2242..3009
                     /locus_tag="VGM72_08105"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEY4265265.1"
                     /translation="MRDIERTLRLGVNIDHVATLRNARGGPHPDPLRAAIAAMAAGAD
                     GITAHLREDRRHINDADLAALVREKLPVNLEMAATDEMLQIALKARPHACCIVPERRE
                     ERTTEGGLDAAGGRERLAPAIRTLKAEGIAVTLFIEAEPRQFEAAVSLGVPVVELHTG
                     AYHDAGLMGDAARAAHQLELLRNGARHAASLGLEVHAGHGLTLGNVGAIAAIPEIVEL
                     QIGHFLVGEAIFSGLASAIARMRAIMDEARARAREAS"
     gene            3006..3428
                     /gene="acpS"
                     /locus_tag="VGM72_08110"
     CDS             3006..3428
                     /gene="acpS"
                     /locus_tag="VGM72_08110"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEY4265266.1"
                     /translation="MIIGLGSDLVDIRRIEKTLERYGTRFTRRIFTELEQKKSERRAQ
                     RAATYAKRFAAKEACAKALGTGLRAGVFWRDMGVVNLPTGQPAIRLTGGAAKRLAAIT
                     PKGTMAQISLTLTDDYPLAQAIVIITAVAEPPQNKPNS"
     gene            3535..4326
                     /gene="lepB"
                     /locus_tag="VGM72_08115"
     CDS             3535..4326
                     /gene="lepB"
                     /locus_tag="VGM72_08115"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY4265267.1"
                     /translation="MAPDDLNQTATATPPASASHKPAKKQESWLEVVKTIIYALLIAG
                     VIRSFVFQPFNIPSGSMEATLLVGDYLFVSKAAYGYSRYSFPFGAMPFSGRFMASAPT
                     RGDVVVFKFPGDNSTDYIKRLIGLPGDRIQMKDGVLYINDKAIPKVPVADYVEDFGGQ
                     PHHVPQFRETLPNGVSYNVLDRYPGGELDTTQVFIVPEGHYFMMGDNRDNSDDSREHV
                     GYVPFENFVGKAEVIFFSTNGSAHFWEFWKWPFAIRYGRIASLVR"
     gene            4374..5039
                     /gene="rnc"
                     /locus_tag="VGM72_08120"
     CDS             4374..5039
                     /gene="rnc"
                     /locus_tag="VGM72_08120"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEY4265268.1"
                     /translation="MLETRLGHEFSDPELLRLALSHASIGARSNERLEFLGDRVLGLI
                     VAEWLYADYPDIDEGGLAVRLNALVRRDSCAKAGEAAGLAAHLIMAASESGSGGRQKG
                     AILAGACEAVIAALYLDGGFAAAKRFVLTYWEEQFASLKPELRDAKTALQEWAQSGAL
                     PRKTQPAYAVKERSGPDHAPVFTVEVRIAGHEGETGEGSTKREAEQDAARRMLERLGL
                     WKA"
     gene            5036..5962
                     /gene="era"
                     /locus_tag="VGM72_08125"
     CDS             5036..5962
                     /gene="era"
                     /locus_tag="VGM72_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003521131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEY4265269.1"
                     /translation="MSENSRCGFAAVLGAPNAGKSTLVNALVGQKVTIVTQKVQTTRI
                     PVRGIAMRGDTQIVFVDTPGIFAPKRRLDRAMVKAAWGGAADADVILVIVDSPELVAH
                     PNGLAGGDTERIVEGLKEAGKKAALLLNKIDGMRRDTLLPLADRLNRSSIFERIFMIS
                     AAKGDGLSDLLDYCASLMPQGALLYPADQVADIPSRLLAAEITREKLYLRLHDELPYA
                     STVSTESWKEQKDGSVRIDQTIFVEREGQKGIVIGKGGQAIKAIGEAARHEMEQLFGR
                     RVHLFLHSKVAERWGETPAHYQEWGLEFPKDQ"
     gene            6031..6777
                     /gene="recO"
                     /locus_tag="VGM72_08130"
     CDS             6031..6777
                     /gene="recO"
                     /locus_tag="VGM72_08130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEY4265270.1"
                     /translation="MEWSDEAIVLSLRAHGESSGILEALTRDHGRHLGLVRGGASSKG
                     RAMLQPGNRVRLTWRARLSEHLGIYTAELALARASDMFERRAALCGLNALSAVAGAVL
                     PEREPHEAAYQGADALLDTIAAHDFADWAPLFVHWEIGLLNELGFGLDLARCAATGSA
                     DDLIYVSPRTGRAVSRGGGEPYRGRLLNLPAFLLGRQAGEASFEDLLAGFQLTAHFLD
                     QWVLTPHNKSMPEARVRLTDLAVRASTQSQ"
     gene            complement(6788..7282)
                     /locus_tag="VGM72_08135"
     CDS             complement(6788..7282)
                     /locus_tag="VGM72_08135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY4265271.1"
                     /translation="MSFSTTDLGTFASLNSVQAGGIHPKPGQTTGGNGLAVGEEVEFD
                     VNFSIALSLPADHYFFVPEVALNTGDFLWLSAARPIVGSGTPFPTGFTDLQSWTRDEA
                     LDPDWLRVGTDIVGAGTFNQAFSLTGTTGTGTTSVPEPGTVTLFGAGLAGLGALRRRR
                     RAKR"
BASE COUNT         1469 a         2223 c         2383 g         1413 t
ORIGIN      
        1 ccacgatggc atgtgtggtg cgccccatga cggcccggcg ccaaaagctg atgcggcaaa
       61 cgacgctcat cgagcgcgtt cgggcctatg atcccggcgc cgacgaatcc atcctcaaca
      121 aagcctacgt cttcgccatg caggctcatg gtccgcaaag gcgtgcgtcc ggcgatccct
      181 atttctcgca tcctttggaa gttgcgggaa tcctcaccga tctgaagctc gataccgcga
      241 ccatcgcgac ggcgctcctg cacgatacgg tggaagacac cagcgccacc atcaaggaca
      301 tcgagagcaa tttcggcggt gaaatcgcag ggctggtcga tggagtcacc aaactctcgc
      361 agctcgaact gttctccgaa cgcaccaagc aggccgagaa tttccgcaaa ctgatgctgg
      421 cgatgtccaa cgacatccgc gtgctgctgg taaagctggc agaccgcctg cacaacatgc
      481 gaacgctctc gcacatcaag agtccggagg tgcgccatcg catcgcgcag gagaccatcg
      541 agatttatgc tccgctcgcg ggtcgcatcg gcatgcagaa catgcgcgag gagctggagg
      601 acctggcctt cgccgaactc gatccggagg cacgcgaatc catcgtgcgc cggatggaac
      661 ggctcgatgc gcaatcctcc gcccgcatcc agcgcatcgc cgacgagatc aagcgcaagc
      721 ttgccgaaaa tggcctcgac gcctgggtct tgggacgcgc caagcggcct tattcgatct
      781 ggcgcaagct gaaagacaag aagagcaatt tcgagcagct ttccgacgtc ttcggattcc
      841 gggccgtggt gggctcggta gaggattgct accgcgcctt gggcgttctt caccggtcct
      901 ggcggatggt gcccgagcga ttcaaggact tcatctcgct gcccaagtcg aacggctacc
      961 gttcgattca caccacggtg atggggccgg agaaccagcg cgtcgaagtt cagattcgca
     1021 cccaggaaat gcacgaggtg gccgagcgcg gcgtcgccgc ccattggcgt taccgcgaaa
     1081 agatcaacga gaacgacccg accgcgatgc gagcctatga atggctgcgc gacatggtag
     1141 acctgctcga acgcggtgag agtccggaag agttctatga gcattccaag ctcagccttt
     1201 atcaggacca ggtcttctgc ttcacgccca aaggcatgct gatcgcgctg ccgcgcggcg
     1261 caacccctat cgatttcgcc tatttcgtac acaccgatgt tggcaacacg gcggtcggcg
     1321 cgaaggtgaa tggcgttcat gtgccgctgc agaccccctt gaagaacggc gatcaggtcg
     1381 agatcatcgg ctcgcgcgag cagagccctt cgcctctctg ggaacgcttc gtggtgacgg
     1441 gacgcgcgcg cgccgaaatc cgccgctatc tacgccatgc ccaacgcggc cagcatgttt
     1501 cgctcgggcg caagattttg gcgaagatct tcgccgatga aggttttgaa ctgaccgaca
     1561 aggcggtgga gggggtgctc agaaagctgc gactctccaa ggcggaggat gtctatgccg
     1621 atgtcggacg cggcgcgctg cgcgccgacg aggtgctgca ggcggtctat ccggagctga
     1681 agcgtgatcc caaacgcaag cgcgcgagct atctggagcc aaattccgcg ggcgtttcga
     1741 tcggcggact gacggagggc atcggctacc gtctgggctc atgctgccat cccatcccag
     1801 gcgatcgcat catcggactg atggtgccgg gcgagggctg cgtcatccac acagtggatt
     1861 gcgacgtgct ggatcgccat caagacagca tggccgattg gatcgacgtg cgttggaagg
     1921 atcatggcgg tgcgttatcg gtggcgcgca tcgtggtcag gctgaagaat tccaccggcg
     1981 cgctggcgac gcttgccaat gtcatcagcc ataatggcgc gaatatctcg aacctgaaga
     2041 tcaccagccg aaatccgctc tatttcgaat tccaggtcga tgcggaagtg cgcgacgcgg
     2101 cgcatctgga gaatttgatc cgggccttga aggtggattc ggcggtggag gcggtggaac
     2161 gcgtgcgcag tctcgatacc gacgatgccg gcgacgggcc tccgccggtt agcgaaaccc
     2221 agggcctctt gccgttcgaa catgcgagac attgagcgaa ccttgcggct gggcgtgaac
     2281 atcgatcacg tcgcaaccct gcgcaatgcg cgcggtgggc cgcatcccga tcctttgcgc
     2341 gcagcgattg ccgcgatggc cgcaggtgcc gacggcatca cggcgcactt gcgcgaagac
     2401 cgccgtcata tcaacgatgc ggatctcgcc gcgctggtgc gtgagaaact gccggtcaat
     2461 ctcgagatgg ccgcgaccga tgagatgctg caaatcgcgt tgaaggcccg accgcatgcc
     2521 tgttgcattg tccctgaacg gcgtgaggag cgcaccacag aaggcggcct cgatgcggcc
     2581 ggcgggcgcg agcggcttgc accggcgatc aggaccctga aggcggaagg tattgcggtc
     2641 acgcttttca tcgaggcgga gccaagacaa ttcgaggctg ctgtctcgct gggcgtgccg
     2701 gttgtcgaac ttcacaccgg cgcctatcac gatgcgggcc taatgggcga cgccgcccgc
     2761 gccgcgcatc agctcgagct tctgcgcaac ggggcgcggc atgcggcgag cctcggtttg
     2821 gaagttcatg cgggacatgg cctgacatta gggaatgtgg gcgccatcgc cgccattccg
     2881 gaaatcgtgg agctgcagat cggtcacttt cttgtggggg aggcgatatt ctcggggctc
     2941 gcgtctgcga ttgcccgcat gcgcgccatc atggacgagg cgcgagcaag agcgcgggag
     3001 gcatcatgat cattggactg ggcagcgatc tcgtggacat ccggcgcatc gagaagacgc
     3061 ttgaacgtta tgggacgcgc ttcacccgcc gcatcttcac cgaactcgaa cagaagaaat
     3121 ccgaacgccg tgcacaacgg gcggcaacct atgccaagcg tttcgcggcc aaggaagctt
     3181 gcgcgaaggc actgggcacg ggccttcggg ctggggtttt ctggcgcgac atgggtgtcg
     3241 tcaaccttcc caccggccag ccggcgataa ggcttaccgg cggcgcggcg aaacggctgg
     3301 ccgcgatcac gcctaagggg acgatggccc agatcagctt gaccctgacc gacgattatc
     3361 cgcttgctca ggcaatcgtg attatcaccg ctgtggctga accgccgcag aacaagccga
     3421 attcataaac tatatatatt tgtaattgcc cctggtgccc ttgacgcgta acaaccgctc
     3481 cgcttccttg gctgcaagcc cgaaccccgg cgaacaagaa aaggccagct ttccatggcg
     3541 cctgacgacc tgaaccagac cgcgacggcg acgcccccag cgagcgccag ccataagcct
     3601 gccaaaaaac aggaatcctg gctggaggtg gtgaaaacca tcatctatgc gcttctcatc
     3661 gctggggtga tccgttcttt cgtgttccag cccttcaaca tcccgtcggg atccatggag
     3721 gctacgctcc tggtgggcga ttatctcttc gtctccaagg cggcttacgg ctacagccgc
     3781 tattccttcc ccttcggggc gatgccgttc tcgggccggt tcatggcatc ggcgccgacg
     3841 cgcggcgacg tggtggtgtt caaattcccg ggcgacaatt cgaccgatta catcaaacgg
     3901 ctgatcgggc ttccgggcga ccgcatccag atgaaggacg gcgtgctcta catcaacgac
     3961 aaggccatcc cgaaagtccc ggtggcggac tatgtcgagg atttcggcgg ccagccgcat
     4021 catgtgccgc aattccgtga aaccctgccc aacggggtca gctacaatgt gctcgatcgt
     4081 tatcccggtg gcgaactcga cacgacgcaa gtcttcattg tgcccgaagg tcattatttc
     4141 atgatgggcg acaatcgcga caattccgac gatagccgcg agcatgtcgg ctatgttccg
     4201 ttcgagaatt ttgtcggcaa ggcggaagtg atcttcttct cgaccaatgg cagcgcgcat
     4261 ttctgggagt tctggaaatg gcccttcgcc attcgctatg gccggattgc ctcgctcgtc
     4321 cgttgaggag cgcccgcggc acggagaaac ggtacggggc tgcggacctg aaggtgctgg
     4381 aaacgcgcct cggtcatgaa ttttcggatc cggaacttct caggcttgcg ctctcccatg
     4441 ccagcatcgg ggcgcggtcc aacgagcggc tggaatttct tggcgaccgt gtgctcggac
     4501 tgatcgtggc cgaatggctt tatgccgatt atcccgacat cgacgaaggc gggctcgcgg
     4561 ttcggttgaa cgcgctggtc cgccgcgatt cctgcgccaa agcgggagag gcggcgggac
     4621 tcgcggccca tctcatcatg gcggcctcgg aatcgggaag cggcggccgg caaaagggcg
     4681 cgatccttgc cggtgcctgc gaagcggtaa tcgcggcgct ttatctcgat ggcggtttcg
     4741 cggcggcaaa gcgcttcgtg ctgacctatt gggaagaaca attcgcgagt ttgaagcccg
     4801 aattgcggga cgcaaagacg gccctgcagg aatgggcgca atccggagcg cttcccagga
     4861 agacgcaacc cgcctatgcc gtgaaggagc gcagcggccc cgatcacgcg ccggtcttca
     4921 cggtagaggt gcgcattgcg ggccatgagg gtgaaacggg cgaaggctcg accaaacgcg
     4981 aagccgaaca agatgcggcg cgtcgcatgc tggagcgtct aggcctatgg aaagcatgag
     5041 cgagaattcg cgttgcggct tcgccgcggt cttgggcgcc cccaatgcgg gtaagtccac
     5101 gctcgtcaat gcgctggtgg gccagaaggt caccatcgtg acccagaagg tgcagaccac
     5161 ccgcattccg gtgcgcggca tcgcgatgcg gggcgacacg cagatcgtgt tcgtcgacac
     5221 gcccggtatt ttcgcgccca aacggcggct ggaccgggcc atggtgaaag ccgcctgggg
     5281 cggggctgcg gatgccgacg taattctggt catcgtggat tcgcccgaac tcgtcgcgca
     5341 tccgaacggg cttgcaggcg gcgacaccga acggatcgtc gaagggctga aagaggcggg
     5401 gaaaaaagcg gctctcctgc tcaacaagat tgacggcatg cgccgcgata cgctgttgcc
     5461 gctggccgac cgattgaatc ggtccagcat tttcgaacgc atcttcatga tctctgcggc
     5521 aaagggcgac ggtctgagcg accttttgga ttattgcgca tctttgatgc cgcaaggggc
     5581 attgttgtac cctgccgatc aggttgccga tattccctcg cgccttctcg ctgccgagat
     5641 cacgcgcgaa aagctctacc tgcgcctgca cgatgaattg ccttatgcct cgactgtgag
     5701 caccgaaagc tggaaggaac agaaggatgg ctcggttcgc atcgaccaga cgattttcgt
     5761 cgagcgagag ggccagaagg ggatcgtcat cggtaaaggc gggcaggcga tcaaggccat
     5821 cggcgaagca gcgcgccatg agatggagca attgttcggt cggcgggtgc atcttttcct
     5881 ccacagcaag gtcgccgaac gctggggaga aactccggcc cactatcagg agtggggtct
     5941 ggaatttcct aaagatcaat gagttgagga gttatcctca gcctgtgcat gaaattcgtt
     6001 caccgtttgt tggtctgtgg atgagcacgc atggaatggt ctgacgaggc gatcgtcctt
     6061 tccttgcgcg cgcatgggga atcctccggc atcctggagg cgttgacccg cgatcacggg
     6121 cggcatcttg gcctggtgcg cggcggcgcg tcgtcgaagg gccgcgccat gctgcagccg
     6181 ggcaaccgcg taaggctcac ctggcgggcg cggttgtcgg aacatctggg aatctatacg
     6241 gcggaactgg ctctcgcgcg ggcctccgac atgtttgaac ggcgtgcggc cctttgtggg
     6301 ctgaacgcgc tctcggcggt tgcaggcgcg gtgctccccg aacgggagcc gcatgaggcg
     6361 gcctatcagg gagccgacgc gttgctcgat accattgcgg cccacgactt tgccgattgg
     6421 gcgccgctgt tcgtgcattg ggagatcggt cttctgaacg agctcggttt tgggctcgat
     6481 ctggcacggt gcgcggcgac aggctcggcc gacgatctga tctatgtttc gccccgtacg
     6541 ggccgcgcag tctcgcgcgg cggcggcgaa ccctatcgtg gccggctatt gaacctgccg
     6601 gcgttcctgc tcgggcggca ggcgggcgag gcgagcttcg aggatctact cgcaggcttc
     6661 cagctgacgg cgcattttct ggatcagtgg gtgctgacgc cgcacaacaa atccatgccg
     6721 gaggcgcggg tgaggctcac cgatcttgcc gtccgcgcct caacgcaatc gcaataaact
     6781 cctttgttta tcgcttcgcc ctgcgacggc gtcgaagggc accaagcccg gcgaggcccg
     6841 cgccgaagag ggtgacggtg cctggttctg ggacgctggt cgtgcccgtg ccggtggtcc
     6901 cggtgagaga aaacgcctga ttaaaggtac cggcgcccac gatgtctgtg ccgacccgca
     6961 gccaatcggg atcgagggct tcgtcgcgag tccaactctg caaatccgtg aatcccgtcg
     7021 ggaaaggcgt gcccgaccca acaatcggcc tggccgcgga aagccatagg aaatcacccg
     7081 tattcaatgc gacctcaggc acaaagaaat aatgatcggc cgggagagat agggcgatgc
     7141 tgaaattcac atcgaattcg acttcttcgc caacggccag tccgtttccg cctgtcgtct
     7201 ggcccggctt tgggtggata ccgccagcct gcacgctgtt gagcgacgcg aaggtcccca
     7261 ggtcggtcgt ggaaaaactc aactctcctg ccgaattgtc ccgcgaactg aacgcgacat
     7321 ccgacggcga attcacccgg gtcgggacta gggtctgtac ccacataggt cgttgaaaat
     7381 tttgacgaac agtgattcac cgtgatggga ggtggatcat gcgatttgat ttatccgatg
     7441 aggaatggac gctccttgaa ccgttgatgc cgaagagccg caagagcg
//
LOCUS       DASYEG010000180         2870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_345263, whole genome shotgun sequence.
ACCESSION   DASYEG010000180 DASYEG010000000
VERSION     DASYEG010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345263"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1101)
                     /locus_tag="VGM72_08140"
     CDS             complement(172..1101)
                     /locus_tag="VGM72_08140"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase"
                     /protein_id="HEY4265272.1"
                     /translation="MSAGTVLITGASGFVGAPALAALQARGFRIHAVSRHPHTTTDGA
                     ITWHAADLLDPQQRRSLIDAIGPTHLLHLAWYVEHGKFWTAPENESWVGASLDLLKLF
                     AENGGRRAVLTGSCAEYDWARGSELPFRETDPCRPQTRYGRAKLALMEQASALAANAG
                     FSLAWARFFFMFGCGEDPRRLIPTMIRGLLAHQDVALSSGRQIRDFLDTRDIGAALAA
                     LLAAETVTGPVNVASGRAIALRDIGAMLTELSGMPGRLLQFGQLPDREGEPHSLVADI
                     ARLTDEVRFRPAASLEERLAQCLERHRAAMRHQ"
     gene            complement(1098..1631)
                     /locus_tag="VGM72_08145"
     CDS             complement(1098..1631)
                     /locus_tag="VGM72_08145"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEY4265273.1"
                     /translation="MKLTPIGIAGAFAIAIEPRCDERGYFARLYCCETLRRAGATFGT
                     IRQTSISFNAERCTLRGLHFQAEPAPEGKIVRVSRGRIFDCFVDLRRDSPTHRHWFGT
                     ELSAEAQNALLIPPGCAHGFLTLEDNCAVEYMMDADYVPELARGCRWNDPAFGIVWPE
                     MPRAMSERDRTWPDYAP"
     gene            complement(1628..>2870)
                     /locus_tag="VGM72_08150"
     CDS             complement(1628..>2870)
                     /locus_tag="VGM72_08150"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4265274.1"
                     /translation="MSAPSCRFCGASLDHIVLDLGEQPLANSFLKASDLARPEAKFRL
                     CVRVCDTCLLVQTDTTVPPESIFSDYVYFSSYSNAWLAHAKAYSDAMIQRFALGPQSR
                     VIEVASNDGYLLKNFVECGIPCLGIEPAENVAAVARAAGVPTETQFLGKDTARRIVAR
                     GGAADLVAANNVLAHVPAINDFVAGLRELLADNGVLTVECPHLLRLIAEVQFDTIYHE
                     HFFYFSLAAIELVFAKHGLRLFDVEQLSTHGGSLRVFAERADAKSPRAVSEKLDELRA
                     LEREAGVHRPDYYDGFNRRVTATIASVRNFLAQAHRELKTVVAYGAAAKGNTLLNACG
                     ATAADIAYAVDRNPHKQGLLLPGTHLPVYAPERIAETRPDYVLILPWNLKEEIVQQTK
                     YIADWGGRFAVPIPELRVFAA"
BASE COUNT          568 a          889 c          916 g          497 t
ORIGIN      
        1 cgtccggtgc gtcaggcgcc tggcgccggc cgcttcgttt gcgcccgcgt ggtattgccg
       61 ctcgaagctg ccatggagct ttacggggcg cttcacaatg cggtggcgaa tttggaatcg
      121 cgtggggtcg tcaaacgccg cacgattccg gccaagaccg acaaatagaa actattggtg
      181 ccgcatggcc gcccgatgcc tctccaggca ttgggcgagc cgttcttcca gcgaagcggc
      241 ggggcggaaa cgcacctcgt cggtgaggcg tgcaatgtcg gccactaggc tgtggggctc
      301 gccttcgcgg tcggggagtt ggccgaattg cagcaggcgt cccggcattc cggataattc
      361 cgtcagcatc gcgccgatgt cccgcaacgc gatggcacgg cctgacgcga cattcaccgg
      421 ccccgtcacc gtttccgccg caagcaacgc tgcgagcgca gcgccgatat cgcgcgtatc
      481 gagaaaatcg cgaatttggc gccccgaact cagcgccaca tcctggtggg caagaagacc
      541 gcggatcatc gtcgggatca gccggcgcgg atcctcgccg cacccgaaca tgaagaagaa
      601 ccgcgcccag gcgagcgaga atcctgcatt ggcggccaga gcgcttgcct gttccatcag
      661 cgccagtttg gcgcggccgt atcgagtttg cgggcggcac gggtcggttt cgcgaaatgg
      721 taactcgctg ccgcgcgccc aatcatattc cgcgcaggaa ccggtcagga cggcgcggcg
      781 gccgccattt tcggcaaaca gcttcagaag atcgaggctc gcgccgaccc aactttcatt
      841 ttccggcgcg gtccagaact tgccgtgctc cacataccac gccagatgca gaagatgcgt
      901 ggggccgatt gcatcgatca aagaacggcg ttgttgcgga tcgagcaaat cggctgcgtg
      961 ccatgtgatg gcgccgtccg tcgtcgtgtg gggatgacgc gatactgcgt gtatgcgaaa
     1021 cccgcgcgct tgcagcgcgg ccaaagccgg cgcgccgaca aagcctgaag cgccggtgat
     1081 taggacagtg ccggcgctca cggcgcgtaa tccggccagg tcctgtcgcg ttcgctcatg
     1141 gcgcggggca tctctggcca cacaatcccg aaggcgggat cgttccaacg gcagccgcgg
     1201 gcgagctcgg gcacataatc ggcgtccatc atatattcga ccgcgcaatt gtcttcgagc
     1261 gtgaggaagc catgcgcgca gccgggcggt atcaggagcg cattctgcgc ttccgcgctc
     1321 agctccgtgc cgaaccaatg ccggtgcgtc ggtgaatcgc gccgcaaatc gacgaagcaa
     1381 tcgaagatgc gtccccgcga tacgcgcacg atcttaccct ccggtgcggg ctcagcctga
     1441 aaatgcaatc cgcgcaaggt gcagcgttcc gcgttgaagg agatgctggt ctgccggatc
     1501 gtgccaaacg tcgcgcccgc tctgcgcaag gtctcgcaac aatagagccg cgcgaaataa
     1561 ccccgttcgt cgcatcgcgg ttcgatggca atggcgaagg cgcccgcgat tccgatgggt
     1621 gtcagcttca cgccgcgaac acccgcagct cgggtatcgg aaccgcgaag cggccgcccc
     1681 aatccgcgat atatttggtt tgctgcacga tctcctcctt caaattccac ggaaggatca
     1741 gaacgtaatc gggccgggtt tcggcaatgc gctccggagc ataaacgggc aggtgcgtgc
     1801 cgggaagcag caggccctgt ttgtgcggat tgcggtcaac ggcataggcg atatcggcag
     1861 ccgtagcacc gcaggcattg agcagcgtgt tgcccttggc cgcggcgcca taggccacca
     1921 cggttttcaa ttccctatgc gcttgagcga ggaagttgcg caccgaggca atggttgccg
     1981 tcacgcgccg gttgaagccg tcataataat caggcctgtg cacccccgcc tcgcgctcaa
     2041 gcgcgcgaag ctcatccaat ttctccgaaa cagcgcgcgg cgatttggcg tcggcgcgtt
     2101 cggcgaatac gcgcagcgag ccgccatggg tggaaagctg ttcgacatcg aacagccgca
     2161 aaccatgttt ggcgaacaca agctcgatgg cggccagcga gaaatagaag aaatgctcgt
     2221 gatagatggt gtcgaactgc acctcggcga tcaggcgcag cagatgcgga cattccacgg
     2281 tcagaacgcc gttgtcggcg agcagctcgc gcaagcccgc gacgaagtcg ttgatggccg
     2341 ggacatgggc gagaacattg ttggccgcga cgagatcggc cgcgccccct cgcgccacga
     2401 tgcgccgggc ggtgtccttg cccagaaact gcgtttccgt cggcacgccc gccgcgcgcg
     2461 cgacagcggc gacattctcg gcgggctcga tgccaagaca cggaattccg cactcgacaa
     2521 agttcttcag aagatagccg tcattgctgg cgacctcgat gacgcggctc tgcggcccca
     2581 atgcgaagcg ctggatcatg gcgtccgaat aggctttggc atgcgccagc caggcattcg
     2641 aataggacga gaaatagacg taatcggaaa agatcgattc cggcggcacg gtcgtgtcgg
     2701 tctgaaccaa caggcatgtg tcgcagacgc gcacgcaaag gcggaatttc gcttcgggcc
     2761 gcgccagatc ggatgctttg agaaaactgt tcgccagcgg ctgttcaccc agatcgagca
     2821 caatgtgatc gagcgacgca ccgcagaagc ggcaggacgg ggcgctcatg
//
LOCUS       DASYEG010000181         4549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3392100, whole genome shotgun sequence.
ACCESSION   DASYEG010000181 DASYEG010000000
VERSION     DASYEG010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3392100"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VGM72_08155"
     CDS             <1..504
                     /locus_tag="VGM72_08155"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY4265275.1"
                     /translation="PDGLYDVVYSNAALQWLGDHERLFRRLFSFVAPGGAMAVQMPRN
                     YDEVCQVLVRETVADPRWAGRLKDVRDWWNGLLPERYYDLMAADARHIDLWETRYFHV
                     LEGEDAVFHWMMGTSLRPFAAALESPLREVFLEEYRVRLAQAYRPRLSGKTLHRFLRL
                     FLVATRS"
     gene            657..854
                     /locus_tag="VGM72_08160"
     CDS             657..854
                     /locus_tag="VGM72_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEY4265276.1"
                     /translation="MAHDSLKSLDTALAHVQQDRRGFLKTLIVGSAVAAIPVMTSQAM
                     AAEGEGGEEGKKKKKKKKEGE"
     gene            963..2117
                     /locus_tag="VGM72_08165"
     CDS             963..2117
                     /locus_tag="VGM72_08165"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY4265277.1"
                     /translation="MPHPKIHPAILLSPVENGYVAYDPVSDRLHQLNPLAALLAELCD
                     GNRSVDEIHGLVAPLLPEGKSGEVERWIDEGLKAGFLTPSEDASPAHRELSAEELYSL
                     SKRLEDNGKVQTAFLCAKRAVELKPDFWDAWYDLGDLGQSVGRREDARQAYQKYFDAH
                     PEDAELEHLLVALRDEPPPPRASDRTIQQIYKGFSVTYETRMRDDLQYQGPERTRDAI
                     LSVMGNPSNLNILDLGCGSGLSGTVLKPWAARLLGVDLSPEMIELARKRGIYDRLEVA
                     EITSWLSEAREPFDLIVSCDVIIYFGDLHRIVAAAAERLRPGGVFALSMERSEHAPFH
                     LTDTGRYAHHPDHVRDAAAAAGLGVGYLDDAFLRMEYGVPVTGLFAALKK"
     gene            complement(2107..4536)
                     /locus_tag="VGM72_08170"
     CDS             complement(2107..4536)
                     /locus_tag="VGM72_08170"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4265278.1"
                     /translation="MRLLHGVPVSVLWLLEDNTEAMRNLKREAEAHGIAANRLVFAPR
                     VSGPDHLARHRVADLFLDTQPYGAHTTASDALWAGLPVLTALGPTFASRVAASLLRAI
                     GMPEMIADSIETYEAMALKLARDPAALAAARAKLSERRETSALFDTARFTRNLESAYI
                     AMWQRWQRSEPARSFAVEAGANRDGTSGQIPDAAAAAYFQGCTLANEGRHQQALAAFE
                     RAIAIAPNFAEALTNRGALLLAAKHHQAALQSFDAALAVNPAMMPAWNNRGNALCELG
                     RYDEAIASYNRVLALSPDVFETVLNRSNALIASRRADEALAGYESALKIRPDSADALK
                     GRANALFELKRFDDAVADYASVLARNPKQDYAQGDLVFSKLQICDWRELNESTDAVIA
                     GVRAGTRVANPFGFLALSSDPQDQKQCAGLWVSDKYPAAAQPVLRGERGAHEKIRIAY
                     LSADFRNHAVAHTMAGVFEHHDRARFETIGVSWGPDDAHDMRARLRDAFCQFIDVDRE
                     SDSQVVSRLRDMKIDITVDLMGFTAESRSAILAARAAPIQINYLGFPGTTNVPAMDYL
                     LADAVVIPEEDRRHYSETVVYLPNSYLPLDGTRRRGAAPSRRQAGLPETGFIFACFNN
                     SFKFSPPVFDIWMRLLRRIEGSVLWLSAVHQAAARNLALEARNRGVDSSRLVFAPYVP
                     GPDDHLARLGLADLFLDTLPYNAHATAADAILAGLPVLTCKGTTFAGRVAASLLYAAG
                     LPELVTESLPAYEALALKLADDPALLASLRNRLAASRTANSAFDTARFTRNLEAAYLK
                     MLEQRRREITS"
BASE COUNT          873 a         1337 c         1429 g          910 t
ORIGIN      
        1 ccggatgggc tgtacgacgt cgtttattcc aatgcagcgc tgcaatggct gggcgatcat
       61 gaacggctat tccggcggct gttctctttc gtcgcgcccg gcggggcgat ggcggtccag
      121 atgccgcgga attacgacga agtctgccag gttctggtgc gggaaaccgt ggccgatcca
      181 cgctgggccg gcagattgaa agacgtgcgc gactggtgga acggacttct gccggagcgc
      241 tattacgatc tcatggccgc tgacgcgcgg cacatcgatc tgtgggagac acgctatttc
      301 catgtgctgg aaggggagga tgccgtcttt cattggatga tggggaccag ccttcgcccc
      361 tttgccgccg cgctcgaatc gccgcttcgg gaggtgtttc ttgaggagta ccgcgttcgg
      421 cttgcgcagg cctaccggcc ccgtttgagc ggcaaaaccc tccaccgttt cctgcgtttg
      481 ttcctggtgg ctacgcggtc ctgagagaaa atgtcaggat atagcaacgt cttagccaga
      541 tttatcacgc agttttggtt gcttgggctt ttttttgttt ggaccatgac aaaaaagtaa
      601 caagccggga taacgcaatc tgggcatatt cggggtctta accagggagg ccatttatgg
      661 ctcatgattc gttgaagtca ctcgacactg cgcttgccca cgtccaacag gaccgtcgtg
      721 gattcctgaa gacgttgatc gtcggctcgg ccgtcgcggc gattcctgtg atgacctcac
      781 aggcgatggc tgctgaaggc gagggcggcg aagaaggcaa gaagaagaag aagaagaaga
      841 aagagggcga gtgatcgctt ctccatctgc ttctttgtaa tctgagcttt tgcatcataa
      901 agacgaccgt gtattcctaa ccggatacac ggtcgtccgt tttcctggac ccctgcccgt
      961 cgatgccgca tccgaagatc catcccgcca tccttctgtc gcccgtcgag aatggctatg
     1021 tggcctacga ccccgtttcc gaccggctgc accaactcaa tcctctcgcc gcgctgctgg
     1081 cagagctttg tgacggaaac cgaagcgtgg atgaaatcca cggccttgtg gcgccgctcc
     1141 tgccggaggg aaaatccggt gaggtcgagc gctggatcga tgaggggttg aaggccggct
     1201 tcctcacccc gagcgaggat gcatccccgg cccatcggga actgtccgcc gaagaacttt
     1261 acagcctttc caagcgtctt gaagacaatg gcaaagtcca gacagcgttc ttgtgcgcca
     1321 agcgcgccgt ggaactgaaa ccggatttct gggatgcctg gtacgatctc ggcgatctgg
     1381 gccagtccgt cggacggcgc gaagacgcgc gccaagccta tcagaaatat ttcgatgccc
     1441 atcccgaaga tgccgaactc gaacatttgc tggtggcgct gcgcgacgag ccgccgccgc
     1501 cgcgcgcctc cgacaggacc atccaacaga tctacaaggg attttccgtt acctatgaaa
     1561 cgcggatgcg cgacgatctg caatatcagg ggccggagcg gactcgcgat gcgattctct
     1621 cggtcatggg aaatccgtca aacctgaata ttctcgatct cggctgtgga tcgggtctgt
     1681 cggggacggt cctcaagcca tgggcggcgc ggcttctggg tgtggatcta tcgccggaaa
     1741 tgatcgagct tgcgaggaag cggggcattt acgaccggct tgaagtcgcc gagatcacgt
     1801 cttggctgag cgaggccagg gagcctttcg atctcatcgt gtcctgcgat gtcatcatct
     1861 atttcggcga tctgcatcgg atcgtcgccg cggccgcaga gaggctgaga cccggcggcg
     1921 ttttcgcgtt gagcatggag cgcagcgaac acgcgccgtt ccatctgacc gacaccggcc
     1981 gctatgcgca ccatcccgat catgtccgag atgcggcggc cgcagcaggt ctcggcgtcg
     2041 gctatctcga cgatgcattt ctgcgcatgg aatatggcgt gccggtcacc ggcctttttg
     2101 ccgcgctcaa gaagtaattt cgcgccgccg ttgttccaac atcttgagat aggcggcttc
     2161 gagattgcgc gtgaagcgcg ccgtatcgaa tgcggaattg gccgtgcggc ttgccgcgag
     2221 acgatttcgc agactcgcca acagcgccgg atcgtccgca agcttcaggg ccagcgcttc
     2281 ataagcaggg agcgattccg tcacgagttc gggcagaccg gccgcataaa gcaggctcgc
     2341 cgcgacacgg cccgcaaaag tggtgccttt gcaggtgagg acgggaagtc cggccagtat
     2401 cgcatctgcg gccgtcgcgt gggcattgta gggcagcgtg tcgagaaaca gatcggcgag
     2461 tccgagccgg gcgagatgat cgtccgggcc gggcacatag ggcgcgaaaa cgagccggct
     2521 cgaatcgaca ccgcgattcc gcgcttccag ggccagattg cgcgcggccg cttgatggac
     2581 ggcggagagc cataacacgc tcccttcgat cctcctgagg agccgcatcc aaatgtcgaa
     2641 tacagggggc gagaatttga agctgttgtt gaagcaagca aagatgaatc ccgtttcagg
     2701 caggccggct tggcgccgcg agggcgcggc tcctcgacgg cgtgtcccat cgagcggcaa
     2761 atagctgttg ggaagataga ccaccgtttc actgtagtgg cggcgatcct cttcgggaat
     2821 cacaacagca tccgcgagca gatagtccat agcgggtaca ttcgtcgtgc ccgggaagcc
     2881 cagataattg atctggatag gcgcggcgcg cgctgcgagg atcgcgctgc ggctttctgc
     2941 ggtgaagccc atcaggtcga ccgtaatgtc gatcttcatg tcgcgcaagc ggctcaccac
     3001 ttgggagtcg ctttcgcggt cgacatcgat gaattggcaa aacgcgtcac gaagacgcgc
     3061 acgcatgtca tgggcatcgt ccggacccca actgacgccg atggtttcga agcgcgcgcg
     3121 gtcgtgatgt tcgaacacgc ccgccatggt gtgagcgacc gcgtggttgc ggaaatcggc
     3181 ggagaggtag gcgatccgga ttttctcatg ggcgccgcgt tctccgcgca ggaccggctg
     3241 tgcggcagcc ggatatttgt cgctcaccca aagcccggca cattgtttct gatcttgcgg
     3301 gtcggaggaa agcgccagga acccgaaggg attggccacg cgcgttcccg cacggacgcc
     3361 cgcaatcacc gcatccgtgc tctcgttcag ctctcgccag tcgcaaatct ggagcttgga
     3421 aaataccaga tcgccttgcg cgtagtcctg ttttggattg cgcgccagaa cgctcgcgta
     3481 gtcggcgacc gcatcgtcga agcgtttcag ttcgaagagg gcattggcgc gccccttcag
     3541 ggcatcggcg ctgtccggcc tgattttgag ggcgctttca tagccggcca gcgcttcgtc
     3601 cgcgcggcgc gaagcgatga gcgcattgct gcgattgaga acggtttcaa agacatcggg
     3661 gctcagggcc agcactctgt tatagctcgc gatggcctcg tcatagcggc cgagttcgca
     3721 gagcgcgttg ccgcgattat tccaggctgg catcatcgcg gggttgaccg cgagagcggc
     3781 atcgaagctt tgcagggccg cctggtgatg ttttgcggcc agcagcagtg ccccacgatt
     3841 ggtgagggct tccgcgaaat ttggcgcaat ggcgatggcg cgctcaaagg cggccaacgc
     3901 ttgttggtgc cgcccctcat ttgccagcgt gcaaccctgg aaataagccg ccgccgcggc
     3961 atccgggatt tgcccactcg tcccgtcgcg attggcgccc gcctccaccg cgaagctccg
     4021 ggccggttcg ctgcgttgcc agcgctgcca catggcgatg taggccgatt ccagattgcg
     4081 cgtgaaacgc gccgtatcaa acagagccga tgtttcgcga cgttccgaaa gcttcgcgcg
     4141 cgcggcggca agtgctgcag gatcgcgtgc gagcttgagc gccatcgcct cataggtctc
     4201 aatcgaatcc gcgatcatct caggcattcc aatcgcacgc agcaaactgg ccgctacgcg
     4261 cgacgcgaag gtcggcccca gcgcggtcag caccggaagg ccggcccata atgcatcgct
     4321 tgccgttgtg tgagcgccat agggctgcgt atcgagaaaa aggtcggcca cgcggtgccg
     4381 ggcaagatga tccggcccgc ttacccgtgg cgcgaacacc agacggttgg cggcgatgcc
     4441 gtgcgcttcc gcctcgcgtt tgagattgcg cattgcctcc gtattgtctt cgagcaacca
     4501 gagaacgctc accggaactc catgcaaaag ccgcatccaa agcgcgaag
//
LOCUS       DASYEG010000182         6950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2231086, whole genome shotgun sequence.
ACCESSION   DASYEG010000182 DASYEG010000000
VERSION     DASYEG010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6950
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2231086"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VGM72_08175"
     CDS             <1..320
                     /locus_tag="VGM72_08175"
                     /inference="COORDINATES: protein motif:HMM:NF022059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEY4265279.1"
                     /translation="TDVVKAIARLAAFYQHESCGQCTPCREGCGWMRRVMDRMVIGDA
                     VTPEIDLLQEVSKEIEGHTICALGDAAAWPIQGLIRHFRPELERRIAARKTETTPMPQ
                     AAE"
     gene            317..937
                     /locus_tag="VGM72_08180"
     CDS             317..937
                     /locus_tag="VGM72_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265280.1"
                     /translation="MTAIMGMGKRENIVAIGLGGAMVLMVVVLFFATHYRALLRTAGL
                     AKENIVAEAANNGLTIPTIDEMCKTVGATAANMAACRDEENQAGEFVIAWMGLNGFLA
                     NGAIDISQIQLAAELGSDATSPLSSPDNSLADPSLAPGLGGGDPNLGDPSAGEPNIDP
                     ATGLPVDQSFDSPAGIAMYCLSNSMDWLQMHDCISRYDPSTRFTGQ"
     gene            943..3066
                     /gene="nuoG"
                     /locus_tag="VGM72_08185"
     CDS             943..3066
                     /gene="nuoG"
                     /locus_tag="VGM72_08185"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEY4265281.1"
                     /translation="MAKLIIDGKEIDADPTLTLLQACEMAGAEIPRFCYHERLSVAGN
                     CRMCLVEWVGSPKPQASCALQVRDIPPNRDGTPAKINTNSPIARKAREGVMEFLLINH
                     PLDCPICDQGGECDLQDQAMGYGRAAFNRYREYKRAVEDKYMGPLVKTSMTRCIHCTR
                     CIRFATEVAGVPDLGATGRGEDMEITTYLEKAIESELSGNVIDLCPVGALLSKPYSFT
                     ARPWELRKTESIDVMDAMGCNIRVDSRGREVMRFLPRVNEAVNEEWLGDKSRFVWDGL
                     KRQRLDQPYIRRDGKLQPASWAEAFDVIASKLKATPAEKMAAIVGDLAAVEEIKALKD
                     LMRGFGVKNLDCRQDGAKLGAVPRQGYLFNTTFQNVEAADALLLIGTNPRWEAPVLNA
                     RIRKTWLNGNLKIANVGAAYDLTYPVQQLSASPSVLEEIAAGTHPFAAALRDAKRPML
                     VIGQGALARADGAAVLNLASEIASSTGMIGPAGTPAEGGWNGFNVLHSAAARVGALDI
                     GFVPDAGGRDVARILDGAGKGEIELIYLLGADEIDADWLGRAFVVYQGTHGDAGAHRA
                     DVILPGAAYTEKSGLYVNAEGRVQLANRATFPPGNAKEDWAILRALSDVAGKRLPYDD
                     IEALRRAIIADAPHFAVRDEAPASAGADPAIWNAIGAAGPIEAHRALESAMADFYLSN
                     PIARASETMAECSRLFVQRTQMAAE"
     gene            3070..4113
                     /gene="nuoH"
                     /locus_tag="VGM72_08190"
     CDS             3070..4113
                     /gene="nuoH"
                     /locus_tag="VGM72_08190"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEY4265282.1"
                     /translation="MIFTQALQGLGVPFGVAWTISQAITIVILLVLLLVMVAFILLFD
                     RKVWAAVQMRRGPNVVGPFGLLQSFADLIKFVLKEIVIPAGANKGVFFLAPILAATLA
                     LSAWAVVPLDNGWVIADINVGILYIFAISSLGVYGIIMGGWASNSKYAFLGSLRSAAQ
                     MVSYEVSIGFILITVLLFAGSLNLSDIVRAQGPDMRGWFVFSPLLPMFVLFFISALAE
                     TNRPPFDLPEAESELVAGFAVEYSSTPFLLFFLAEYVNIVLMCAMMSILFFGGWLPPI
                     DIWPLNAIPGVIWFVLKIWFFFFLFAMVKAIVPRYRYDQLMRIGWKVFLPISLVWVVL
                     MAGYLFAIGRLPG"
     gene            4122..4610
                     /gene="nuoI"
                     /locus_tag="VGM72_08195"
     CDS             4122..4610
                     /gene="nuoI"
                     /locus_tag="VGM72_08195"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEY4265283.1"
                     /translation="MAWLDRTARTIFLWEFVKSFALGMRYFFAPKKTLNYPFERSPLS
                     PRFRGEHALRRYPSGEERCIACKLCEAVCPANCITIEAEPREDGSRRTTRYDIDMTKC
                     IYCGLCAEACPVDAIVEGPNLDFSVETREELYYDKARLLANGDRWEREIAKNLELDAP
                     YR"
     gene            4721..5326
                     /locus_tag="VGM72_08200"
     CDS             4721..5326
                     /locus_tag="VGM72_08200"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005164.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY4265284.1"
                     /translation="MIQAIAFYVFSAVLIASALMVIGARNPVHSVLFLILAFINAAAL
                     FVLLGAEFLAMILVVVYVGAVAVLFLFVVMMLDIDFVALRRGFMEYLPLGALIGLVLM
                     SELMIAVGAWAFGSAAPAARSNPLPPGVTNTEALGRILYTDYIFYFQTAGLILLVAMI
                     GAIVLTLRHRPGVKRQSIARQVARGPATAIEMRDVKPGEGG"
     gene            5330..5638
                     /gene="nuoK"
                     /locus_tag="VGM72_08205"
     CDS             5330..5638
                     /gene="nuoK"
                     /locus_tag="VGM72_08205"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEY4265285.1"
                     /translation="MAIGLPHYLIVAALLFTIGVFGIFINRKNVIVILMSIELILLAV
                     NINLVAFSVFLGDLIGQVFALFVLTVAAAEAAIGLAILVVYFRNRGTIAVEDINLMKG
                     "
     gene            5652..>6950
                     /gene="nuoL"
                     /locus_tag="VGM72_08210"
     CDS             5652..>6950
                     /gene="nuoL"
                     /locus_tag="VGM72_08210"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY4265286.1"
                     /translation="MYTAIVFLPLLGSAIAGLFGRAIGVRASELVTTGLLFVAAVLSC
                     YALYDVNFLGHGLIVQVAPWIASGNFSVDWALRIDALTAVMLVVVTGVSSLVHLYSIG
                     YMHEDPDRPRFFSYLSLFTFAMLMLVTANNFLQMFFGWEGVGLASYLLIGFWYTKPSA
                     NAAAIKAFIVNRVGDFGFALGVFGIYYVFGTLDFDRVFMAAPMFAGKTISFAGYSPDL
                     LTTLCILLFIGAMGKSAQLGLHTWLPDAMEGPTPVSALIHAATMVTAGVFLVCRCSPL
                     FELAPAALAFVTFIGATTAFFAATIGLFQNDIKRVIAYSTCSQLGYMFAAAGVSAYGA
                     SMFHLFTHAFFKALLFLGAGSVIHAMHHEQDMRKMGGLAKRIPITWAMMLIGNLALTG
                     IGIPLLGIGTAGFYSKDAIINATFLSGTGIGAYAFVLTTVS"
BASE COUNT         1176 a         2129 c         2122 g         1523 t
ORIGIN      
        1 cgaccgatgt ggtgaaggcc atcgcgcgtt tggccgcgtt ctatcagcat gaatcctgcg
       61 gccaatgcac gccctgccgt gagggctgcg ggtggatgcg ccgggtcatg gatcgcatgg
      121 tgatcggcga tgcggtcacg ccggaaatcg atctgctgca ggaagtcagc aaggagatcg
      181 aaggccacac catctgcgcg cttggcgatg cggcagcgtg gccgatccag gggctgatcc
      241 ggcatttccg tcccgagctc gagcggcgca ttgccgcacg caagaccgaa accacgccca
      301 tgccacaggc cgcggaatga cggcaatcat gggtatgggc aagcgcgaga acatcgtagc
      361 gatcggcctt ggcggcgcga tggtgctgat ggtggtcgtg ctgttcttcg ccacccatta
      421 ccgggctttg ctgcgcaccg ccggccttgc aaaggaaaac atcgtcgcgg aagcggccaa
      481 taacggcctg accatcccga ccatcgatga gatgtgcaaa acggtgggcg cgacagcggc
      541 caatatggcc gcctgcaggg acgaagaaaa tcaagcaggc gaattcgtca tcgcctggat
      601 gggcctcaac gggtttctcg ccaatggcgc gatcgacatc agccagattc aactcgcagc
      661 cgaactggga agcgatgcaa cctctccctt gagcagtccc gataacagcc ttgccgatcc
      721 aagcctcgcc cccggtctcg gtggcggaga tcccaatctc ggcgatccct ctgcgggcga
      781 accgaacatc gaccccgcga ccggcctgcc ggtggatcag agtttcgatt cgcctgcggg
      841 aatcgcgatg tattgtctga gcaattcgat ggattggctg caaatgcatg actgcatttc
      901 gcgctacgat ccctccaccc gattcaccgg acaatagcgg caatggccaa actcatcatc
      961 gacggcaagg aaatcgacgc cgatcccacg ctgacgctgc ttcaggcctg cgagatggcg
     1021 ggcgctgaga ttccgcgttt ttgctatcac gagcggctgt cggttgccgg caattgccgc
     1081 atgtgcctgg tggagtgggt gggctcgccc aagccgcaag cctcgtgcgc gctgcaagtg
     1141 cgcgatattc cgcccaatcg cgacggcacg ccggccaaga tcaacaccaa ttcgccgatc
     1201 gcccgcaaag cccgcgaagg cgtgatggaa ttcctgctga tcaatcatcc gctcgattgc
     1261 ccgatctgcg atcagggcgg cgaatgcgat ctgcaggacc aggcgatggg ctatggccgc
     1321 gctgccttca accgctatcg cgagtacaag cgcgcggtgg aagacaaata tatgggtccg
     1381 ctggtcaaga ccagcatgac ccgctgcatc cattgcacgc gctgcatacg gttcgcgacg
     1441 gaagtcgcgg gcgtgccgga tctcggcgcc acggggcgcg gcgaggacat ggagatcacg
     1501 acctatctgg agaaagccat cgagtccgag ctttcgggca atgtgatcga tctctgtccg
     1561 gtgggcgcgt tgctgtccaa gccctattcc tttacggcgc ggccatggga attgcgcaag
     1621 accgaaagca tcgacgtgat ggatgcgatg ggctgcaaca tccgcgtcga ttcgcggggc
     1681 cgcgaagtga tgcgattcct gccgcgcgtg aacgaagcgg tgaatgagga atggctcggc
     1741 gacaagtcgc gctttgtctg ggatgggctg aaacgtcagc gcctggacca gccctatatt
     1801 cggcgtgacg gcaagttgca gccggcgagc tgggcggaag ccttcgatgt catcgcgtcg
     1861 aaactgaaag cgacaccggc tgagaagatg gccgccatcg tcggcgatct ggcggcggtg
     1921 gaagagatca aggcgctgaa ggatctgatg cgcggcttcg gggtgaagaa tctcgactgc
     1981 cgccaggatg gcgcgaagct gggcgcggta ccgcgccagg gctatctctt caacacgaca
     2041 ttccagaatg tggaagcggc cgatgcgttg ctcttgatcg gcaccaatcc gcgctgggaa
     2101 gcgcctgtgc tgaatgcgcg catccgcaag acctggctca atggcaatct gaaaatcgcc
     2161 aatgtcggtg ccgcctacga tctgacctat cccgtgcagc aattgagtgc ctcgccgagc
     2221 gtgctcgagg agatcgcagc ggggacgcac ccgttcgcag cggcattgag agacgccaag
     2281 cggccgatgc tggtgatcgg gcagggggcg ctcgcgcgcg ccgatggtgc ggcggtcctt
     2341 aatctcgcga gcgaaatcgc ctcctccacc ggcatgatcg ggcccgccgg cacgcctgcc
     2401 gaaggcggct ggaacggttt caatgtgttg catagcgcgg ccgcgcgcgt gggcgcgctc
     2461 gatatcggtt tcgtgccgga tgcgggaggt cgcgacgtcg cgcgcattct cgatggcgcc
     2521 ggcaagggcg agatcgagct gatttatctc cttggcgcgg atgagatcga cgcggattgg
     2581 ctggggcgtg catttgtcgt ctaccagggc acccatggcg atgccggcgc acaccgcgcc
     2641 gatgtcatcc tgcccggtgc cgcctacacc gaaaaatccg ggctctatgt caacgccgag
     2701 gggcgcgtgc agctcgccaa tcgcgccacc ttcccgcctg gcaatgccaa ggaggattgg
     2761 gcgatcctgc gcgcgttgtc ggatgttgcc ggcaagcgtc tgccttacga tgatatcgag
     2821 gcgctgcgtc gcgccatcat tgccgatgca ccccattttg ccgtgcgcga tgaggcgccc
     2881 gcctcggccg gagcggaccc agccatctgg aatgccatcg gcgcggccgg tcctatcgaa
     2941 gcgcatcgtg cgctcgaaag cgcgatggcc gatttctatc tcagcaaccc gatcgcgcgc
     3001 gcgagcgaga ccatggcgga atgttcgcgc ctcttcgtcc agcgtaccca gatggcggcg
     3061 gaataggcga tgatcttcac ccaagccctc caaggcctcg gcgttccctt tggggtggcg
     3121 tggacgattt cccaggcgat caccatcgtc attcttctgg tcctgctgct ggtgatggta
     3181 gccttcatcc tgctgttcga ccgcaaggtc tgggcggccg tgcagatgcg ccgcggtccc
     3241 aatgtggtgg gtccgttcgg cctgctgcag agtttcgccg atcttatcaa attcgtgctc
     3301 aaggaaatcg tgattccggc gggcgccaac aagggcgtct ttttcctggc gccgatcctg
     3361 gcggccaccc tggcgctatc ggcctgggcc gtggttccac tggacaatgg ctgggtgatt
     3421 gccgacatca atgtcggcat cctctacatc ttcgcgattt cctcgctcgg cgtttacggc
     3481 atcatcatgg gcggctgggc ctcgaattcg aaatatgcct tcctcggctc gctgcgttcg
     3541 gcggcccaga tggtgtccta cgaagtctcc atcggcttca tcctgatcac ggtcttgctg
     3601 ttcgccggct cgctgaatct ttcggatatc gtgcgcgcgc aaggtcccga catgcgcggc
     3661 tggttcgtgt tctcgccgct tttgccgatg ttcgtgctgt tcttcatttc ggcgttggcc
     3721 gaaaccaacc gcccgccctt cgacctgccg gaggccgaat cggaactcgt ggccggcttc
     3781 gcggtggaat attcatcgac gccgttcctg ctctttttcc tcgcggaata cgtcaacatc
     3841 gtcctgatgt gcgcgatgat gtcgatcctg ttcttcggcg gttggctccc gcccatcgac
     3901 atatggccgc tcaacgccat acccggcgtg atttggttcg tgctcaagat ctggttcttt
     3961 ttcttcctgt tcgcgatggt gaaggcgatc gtgccgcgct atcgctacga ccaattgatg
     4021 cggatcggct ggaaggtctt cctgccgatt tcgctcgtat gggtggtgct catggcgggt
     4081 tatctgttcg ccatcggcag gctccccggg tgagggcata gatggcttgg ctggaccgca
     4141 ccgcgcgcac gatcttcctc tgggagttcg tcaagagttt cgcccttggg atgcgctatt
     4201 tcttcgcgcc caagaaaacg ctgaactatc ccttcgagcg ctcgccgctg tcgccgcgtt
     4261 tccggggcga gcatgcgcta cgccgttatc cgtcgggcga ggaacgctgc atcgcctgca
     4321 agctgtgcga ggctgtgtgc ccggccaatt gcatcacgat cgaagctgag ccccgtgaag
     4381 acggcagccg ccgcaccacc cgctacgaca tcgacatgac gaaatgcatc tattgcggcc
     4441 tctgcgcgga ggcctgtccg gtggatgcca tcgtcgaggg tccgaacctc gatttttcgg
     4501 tcgagacccg cgaagaactt tattacgaca aggcaagact gctcgcgaac ggcgatcgct
     4561 gggaacgcga gatcgctaaa aatctggaat tggacgcccc ttaccgataa ctctctcccc
     4621 ctcatccttc ggcaagctca ggatgaggat ggctttctgc aactcctcat gctgagcctg
     4681 tcgaagcatg aggtcctacc cgagtgaacg aacgcatttc atgattcagg ccatcgcctt
     4741 ttatgttttc tcagcggtgc tgatcgcctc cgcgttgatg gtgatcggcg cgcgcaatcc
     4801 cgtgcattcg gtgctgttcc tgatccttgc cttcatcaat gccgccgccc tgttcgtgct
     4861 tctgggtgcc gaattcctcg cgatgatcct ggttgtcgtc tatgtcggcg cggtcgcggt
     4921 gctattcctc tttgtcgtca tgatgctcga tatcgatttc gtggcgctga ggcgcggctt
     4981 catggaatat ttgccgctcg gcgcgctgat cggcctggtg ctgatgagcg aacttatgat
     5041 cgcggtgggc gcctgggcat tcggcagcgc cgcgccggcc gcacgctcta atcccttgcc
     5101 gcccggggtc accaataccg aagcgctggg ccgcatcctc tacaccgact acatcttcta
     5161 tttccagacc gcggggctga tcctgctcgt cgcgatgatc ggcgccatcg ttctgacgct
     5221 gcgccaccgt cccggcgtca agcgccaatc cattgcgcgt caggtggcgc gcgggccggc
     5281 gaccgcgatc gaaatgcgcg acgtcaagcc gggggagggt ggctaaaaca tggcgatagg
     5341 acttccccat tatctgatcg tcgcggcact cctcttcacc attggggtgt tcggcatttt
     5401 catcaaccgt aagaacgtca tcgtcatcct gatgtccatc gagctgatcc tgctcgcggt
     5461 gaacatcaac ctcgtcgcgt tctctgtgtt tctcggcgat ctgatcggcc aggtgtttgc
     5521 gcttttcgtg ttgacggtcg ctgccgccga agccgccatt ggattggcga tccttgttgt
     5581 ctatttccgc aaccgcggca cgattgcggt tgaggacatc aatctcatga agggctgagc
     5641 gcgaaaactt catgtatacc gccatcgtct ttcttcccct tctcggctcc gcgatcgcgg
     5701 ggcttttcgg tcgcgccatc ggcgtgaggg cttccgaact cgtgacgacg ggattgttgt
     5761 tcgtggcggc cgtgctttcc tgttatgcgc tatacgacgt gaatttcctc ggccacggcc
     5821 tgatcgtcca ggttgcgccc tggatcgcat cgggaaattt cagcgtcgat tgggctctgc
     5881 gcatcgatgc gctgaccgcc gtgatgctcg tggtggtgac gggtgtgtct tcgctcgtgc
     5941 atctctattc catcggctat atgcacgaag acccggaccg cccgcggttc ttctcctatt
     6001 tgtcgctgtt caccttcgcc atgctgatgc tggtgacagc caacaatttc ctgcagatgt
     6061 tcttcggctg ggaaggtgtc gggctggcgt cctatctgct catcggcttc tggtatacga
     6121 agccttccgc gaatgcggcc gcgatcaagg ccttcatcgt gaatcgcgtc ggcgatttcg
     6181 gcttcgcgct tggcgtgttc ggaatctatt acgtgttcgg gacgctcgat ttcgaccgcg
     6241 tgttcatggc cgcgcccatg ttcgccggca agacgattag cttcgccggc tattcgcccg
     6301 acctgctgac gacgctgtgc atcctcctgt tcatcggtgc gatgggaaaa tccgcgcaat
     6361 tgggcctgca cacctggctg cccgacgcta tggaaggccc gaccccggtc tcggccctca
     6421 tccatgccgc gacgatggtg acggcgggcg tgttcctcgt ttgccgctgc tcgccgctgt
     6481 ttgagctggc gccggcggcg cttgccttcg tcaccttcat cggtgcaacg acggcttttt
     6541 tcgcggccac cattggtctt ttccagaacg acattaagcg tgtgatcgcg tactcgacct
     6601 gttcgcagct cggctacatg ttcgccgcgg ccggcgtctc ggcctatggc gcgtccatgt
     6661 tccacctttt cactcatgcc tttttcaagg ccttgttgtt cctgggcgcc ggctcggtga
     6721 tccatgccat gcatcatgag caggacatgc gcaaaatggg cggtctggcc aagcgcattc
     6781 cgatcacctg ggcgatgatg ctgatcggca atctcgcgtt gacgggaata ggaattccgc
     6841 tgctcggcat cggcaccgcg ggcttctatt ccaaggacgc catcatcaac gcgaccttcc
     6901 tgtcgggaac cggcatcggc gcctatgcct ttgtgctgac gaccgtctcg
//
LOCUS       DASYEG010000183        29639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3537735, whole genome shotgun sequence.
ACCESSION   DASYEG010000183 DASYEG010000000
VERSION     DASYEG010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 29639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29639
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3537735"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..509
                     /locus_tag="VGM72_08215"
     CDS             213..509
                     /locus_tag="VGM72_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265287.1"
                     /translation="MFQLTHNWELPPIGLSVPGCVEEAIHQCARGESSPDLALMQLLV
                     ASCSEEETERVLGTAIWDALEDRNAGTAERLGAVQKLWDGAKDLVQSAAPSLCR"
     gene            606..983
                     /locus_tag="VGM72_08220"
     CDS             606..983
                     /locus_tag="VGM72_08220"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEY4265288.1"
                     /translation="MEIATEITFHNMRSSEGLRDDILKRIAKLEKFCGRLISCRVSIE
                     ARTKNHRTGNIYEVHIEMQAPGNTLVVSREPHHVRERRAHSDVRTSIIEAFAAAESQL
                     RSFGKKKTGEVKTAKRGDSTQTL"
     gene            1039..1746
                     /locus_tag="VGM72_08225"
     CDS             1039..1746
                     /locus_tag="VGM72_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEY4265289.1"
                     /translation="MDNYEGGDFDNRADAGRKLARALEHLRPEHPVVLALPRGGVPVG
                     FEVARALQAPLDVVLVRKIGSPGQPELGLGAVVDGANPQLVLNEELVGLVQPGRRYLE
                     AEEKRQLAEIERRRALYRPGRAPISLAGRIVIVVDDGIATGATMKAVLQALSQSEPKR
                     VVLAVPVAAADSLEELAKLADETVCLMTPAPFYSVGAFYRDFTQTADQDVIDLLRRAE
                     TLVPKEAETAAPIASDE"
     gene            complement(1727..2182)
                     /locus_tag="VGM72_08230"
     CDS             complement(1727..2182)
                     /locus_tag="VGM72_08230"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY4265290.1"
                     /translation="MMKFAVATLFIGFAGPAIAGDISPEANRELVAAHAKMPGVMVLP
                     SGLQYRVIKSGTGETPTANDTVTVSYKGTRVDGYVFDSTKPGETRDLPVAKVIPGWIE
                     ALSLMKAGDEWELLVPAKLAYGEERNNSDLWGQALLFDVQLVAVIHPKQ"
     gene            complement(2215..5046)
                     /locus_tag="VGM72_08235"
     CDS             complement(2215..5046)
                     /locus_tag="VGM72_08235"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEY4265291.1"
                     /translation="MRTMGLLSRVALAALLISLSGPARLATAADSAAAAWPQAKSDLP
                     ADSDVRFGALPNGMRYAIQKNATPKGEVSFRLRIGAGSLMESDAQRGLAHFLEHMAFR
                     GSTHVPEGDVFQMLQRIGLRVGADANASTGQTETIYQFDVPASDKTTLDTAFTLMRDI
                     ATELSLKPEAMDAERGPVLSEERLRDTPGMRAFEAQNAYLLKGQLAPERSPIGKVDII
                     KTAPVSQIADFYHAYYRPERATLVIVGDIDPDVIEKEIKTRFSDWKQAGAGRSDPNLG
                     TPLTRGQETKVFVETSAPQSVSAAWTGPYDGAPDTKAHETRDLIERIGFGILNQRLAQ
                     AAQSPDAPFLAAGASRGNVSRSARIASLRVSYAADKWQRAFEEADKIRRQVLAQGVTQ
                     QELDRQITNMLTGAQSAVASASTRASRGIANGILSSVDTDSVFTSDATDLATIQDALK
                     GLTIEQVNTALRGAFAGNGPLLFLSSPKPVEGGEAALASVWNKAESEKIENAPQLQLA
                     VWPYTSFGVAGQVVETRHIDDLDATLIRFENGVRLTVKPTKYRADQILVSVDIAGGDL
                     AFPKDRIVIDTGAYLSGGLEALSYLDLRRTLSGKITSVGFGVDDDAFSLSGATRPADL
                     DTEMQVLAAYITKPGWRPEPFEQGLSSLTDNLTKLDATPMSLFGAKFAELLHPGDARW
                     AYPTLADVQTAKLDQVKAIIAPALANGPIEVTMVGDVSVEQATKAVAATFGALPRRSG
                     APMALPAAGDLKFPAPTPEPIVLHHAGRADQGVAAIAWQTTDVYANSESAARRVVTDI
                     LQFRLMEQLRIKFGATYSPSATASASRTFPGYGYIAAYAEIPPEKAQLFFDTVKQVTA
                     DLRAREPSKDEFERARKPELDTLEKAVQTNNYWLGSLSGAQTDERRLKLIRDARPGLE
                     TVTPADVQRVAREYLTDERAWKLIVVPKP"
     gene            5266..6030
                     /locus_tag="VGM72_08240"
     CDS             5266..6030
                     /locus_tag="VGM72_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY4265292.1"
                     /translation="MSIEDGLRFRIARACRVLGKMELTRALRGHISARIPGTDRIFIR
                     ARGPAESGVRYTTEDEVVEIGLDGKPVAGMPQGLRTPSEVFIHTEIFRARPRVNAVLH
                     LHPPIVVLFTVTGVPLLPICGAYDPQGLGLALEEPPTFESSVLIDTPELGRELSAVIG
                     DKRACLMRGHGITTAADSIEEAAVVALSLNELATMNYQARALGTPRPISESDREALSP
                     AMKRLMKRGPRPGEPGDSVVTEWRYYCRLTGEDGGT"
     gene            6133..8193
                     /locus_tag="VGM72_08245"
     CDS             6133..8193
                     /locus_tag="VGM72_08245"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HEY4265293.1"
                     /translation="MRLKTLLIWLLACAAGPACADEGMWTFDNFPAAQVKQKYGVTID
                     QKWLDRVQASAVRLAGGCSASVVSPNGLVLTNHHCVAGCAQNLSTPEHDYVKNGFAAA
                     RAEDEKQCPGVQAEILQTIADVTPAINAATADKAGDGFQRARDAAVANIEKQACTGRE
                     ATQRCQVITLYDGGQYKLYTYRKYSDVRLVFAVEFDTAFFGGDPDNFNFPRYDLDFSF
                     LRLYENGKTVATPEHLTWNPSAPKDGEPVFVAGNPGGTDRQLTESQLVTLRDSTQPFG
                     LILGSELRGRYTRFAEESAEHARIINRDLFGLENGLKAQRGEHEALLNPQLMADKHKA
                     DAELKSRVDADPKLKAEIGDPWAEIAKAQAERVPLAKPYYFIETRAGGGSTLFGYARI
                     LVRAADERTKPNGERLREYADARLPLIERGLLAARPVEPEVEQLKLEFWLTKLREELT
                     ADAPETALVLGKDSPENLSRALIASKLADPSLRKQLWEGGAAAVRASTDPMIRFALKI
                     DAAGRAIRKTYEERVSSPVAAASERIAKARFAVYGTSVYPDATFSLRLSYGKIAGWTE
                     PGRTILPFTNFAGLYERATGQPPFELTPRWVEAQSKLDLKTVFNMSSDNDIIGGNSGS
                     PLIDAKGEVIGAIFDGNIHSLGGDYYFDPKLNRSVTVSTAAITEALNKVYGQTGLISE
                     LTGH"
     gene            8270..9235
                     /locus_tag="VGM72_08250"
     CDS             8270..9235
                     /locus_tag="VGM72_08250"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY4265294.1"
                     /translation="MRKCIAALGFAMLPTLGAWPAIAQTPAKIPYPQKVVILVTHSSP
                     GAGSDVFLRELVRYLQKYINATFVVENDEGGSGAKAVSRVATAKPDGSMLYAATPTYI
                     LTSLLSHPSNTYRDLEPVVNFFTDSEIIYVRAESPYKSLKDLIDHARTSRGRWGAANP
                     GSLERQAAEQLKIAAKVNAAVVSHEGGGDMMINVLNGTLDMGVGEMEEIRAQYEAHKV
                     RVLATFNGTRMPALPDVPTVKESGYNVVLEKFRGLAAPKGLPPAIAKIWDDAAQKILA
                     DPEYRKAYLEDDLIPHYLAHDQYGPFITKFAADTGAFLKSTGVIR"
     gene            9235..9687
                     /locus_tag="VGM72_08255"
     CDS             9235..9687
                     /locus_tag="VGM72_08255"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEY4265295.1"
                     /translation="MRKDLVAAAILLCIAAAYYAASTTIPSSTLEDSVGPRGLPVVLA
                     VLLAMVAIVIGVRALVTAPVSGEAAKDAEAPWPRAIGMLVIGALYIPVANFLGYWLTL
                     VMLLLAVILYEGMKPSWRMLAVALGGATFFFLLFDVLLGVRVPEGMLF"
     gene            9710..11206
                     /locus_tag="VGM72_08260"
     CDS             9710..11206
                     /locus_tag="VGM72_08260"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY4265296.1"
                     /translation="MEEFRHALEMLVTTSVVPAALGGLIWGMLGGATPGLSASITMAL
                     LLPFTYTLSPMPAIVMLATCYIGAEYGGSIPAILIRTPGTNASAATMLDGYEMNRQGR
                     AGEALGLSLWSGVIGGLFGLTMLVLLTEPLSRLALLFRPTTYFGLGILGLSVIASLSS
                     KSLLKGMGAALLGLMVATIGTDPVSGVSRFTFGVPDLLDGIQPLFVMVGLYAVTELMT
                     RAGITPNLIKSNASIRVRFPRFRMMKKVARSQIIGCAVGTFEGLMPGAGGTIAAFIAY
                     DQAKLWSKHPEEFGKGAEEGVAGPEAANNTVASTALIPLLSLGIPASNSAAVLLGGLL
                     IHGLLPGPRLFEQNSDVILGLYTGTFVADIAQVIIGIMVLPVCVWLVNRPKPYRSAFI
                     LALVLSGVYTVHMSLFDVGIVLSAGLLGYFMKYFGFPFLPAVLGVVLGTKLIESNYRR
                     SLVLSGGDFDIFFQDPIAVGFFVVAAIIMLGALYAEWRAAQQRKVSLA"
     gene            11203..12564
                     /locus_tag="VGM72_08265"
     CDS             11203..12564
                     /locus_tag="VGM72_08265"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY4265297.1"
                     /translation="MTISQQLAAFAAATATAQLPQRARETIRLLLLDVAGLCVAARSN
                     DYVAAARGSAVSSGCTTAIGHAGTFGPYDAALINGTAAHGEDYDDTFEGGPVHAGAVI
                     VPAVLAAAEHRGLCGDAVVKGIAAGVEVMCRMSLVAPQATHKACFHPTAIFGAPAAAA
                     GVAAALGLPPEKIAAAIGVAGSLASGIIEYLADGSWTKRLHAGAAAQSGIRAAFLAEG
                     GFTGPLTVLEGGHGFYKAFAPSKVPDFQPLLNRLGTEWVLETVAFKPYACGTMTQPFI
                     DCAIELARSGVHAEDIVSLVCEVGEGTVHRLWEPLLQKQAPPNGYAAKFSTPYCIAAG
                     FVDGKAGFEQFTDARVADPKLCALAGKASYVINPDDPYPKNFTGHIRATLRDGTVREV
                     RKAHMRGGAHEPLSEADIMTKFYDNTRFGGWPRAKAAALAEALDRIVAAGSAAADLST
                     ARE"
     gene            12566..13306
                     /locus_tag="VGM72_08270"
     CDS             12566..13306
                     /locus_tag="VGM72_08270"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4265298.1"
                     /translation="MQELEGSVAIVTGAARNIGRAIALDLADAGAAVTVLARSDIAGV
                     KSVAAEIEGRGGRALAIQGDVTQPDDVRRAVDETVKKFGRLDILVNNAGVRPEGAFED
                     LSLDSWHQVLAVILDGPFLCAQAALPALEKSGRGTIINIGGLTAYTGAAHRAHVITAK
                     AGLDGFTKALAVELAPKGITVNLVAPGMIETKREGAEPVHRKTRTILAPRRGQPEDIS
                     AMVRHLAGPRARSMTGQTIHINGGVYLP"
     gene            complement(13312..13767)
                     /locus_tag="VGM72_08275"
     CDS             complement(13312..13767)
                     /locus_tag="VGM72_08275"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEY4265299.1"
                     /translation="MAILAFASVAAASIAGQIATFPNLAIWYAGLEKPPFNPPNWIFG
                     PVWTALFVLMGFALWRVLRLPTSPQHHNALILFYIQLALNAAWSWMFFAAHSPVLGLI
                     DILPQLTIIVMTIIAFWKLDRLAAWCLVPLAAWVAFAAVLNFSIWYLNG"
     gene            complement(13889..14959)
                     /locus_tag="VGM72_08280"
     CDS             complement(13889..14959)
                     /locus_tag="VGM72_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265300.1"
                     /translation="MAYKILLLGASYGSLLASKLLFGGHSIHLICLPTEADLINAEGF
                     EVRLPIRGRKDPVILKSRNLPGKVTAGPAAGVNPKDYDLVGLAMQEPQYRSPGVRELL
                     DAVARSRVPCMSIMNMPPLPYVKRIPGLNYEALKPAYTDPTVWDNFDPACLTLNSPDP
                     QAIRPPEEKANVLQVTLPTNFKVARFDDEKSTAILRQLEKDVDAARFDTPEGKIELPV
                     KLRVYDSIFVPLAKWAMLCAGNYRTVTADGMRTAKEAVHTDIEASRSVYNFVNDLCVK
                     LGANPSDLVPFEKYAAAAESLTRPASAARALQNGAPNIERADKLVQLIAKQKGLSHPT
                     LDATVALVDSRLEANRKKAAAA"
     gene            complement(14984..15196)
                     /locus_tag="VGM72_08285"
     CDS             complement(14984..15196)
                     /locus_tag="VGM72_08285"
                     /inference="COORDINATES: protein motif:HMM:NF022832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HEY4265301.1"
                     /translation="MANQIADFFRLKPEDQAVAGAADHIRKFWDPHMRAKMAEHLAHG
                     GEGLNLIALKAVQQVCKASGTAAGTH"
     gene            complement(15224..18085)
                     /gene="fdhF"
                     /locus_tag="VGM72_08290"
     CDS             complement(15224..18085)
                     /gene="fdhF"
                     /locus_tag="VGM72_08290"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEY4265302.1"
                     /translation="MTVHTDIDRSPIDRGTPEKAAAQSVTLEIDGISVTVPAGTSIMR
                     ASSLAGIQVPKLCATDSLEPFGSCRLCLVEIAGRRGTPASCTTPAENGMKVSTQTDKL
                     AKLRRGVMELYISDHPLDCLTCAANGDCELQDMAGAVGLREVRYGYDGENHLGQTKDE
                     SNPYFTFDPSKCIVCSRCVRACEEVQGTFALTIDERGFASHVSAGMNEDFLDSECVSC
                     GACVQACPTATLMEKTVIQHGTPERSVITTCAYCGVGCSFEAELQGDRVVRMTPYKDG
                     KANHGHSCVKGRFAWGYATHKERITKPMIREKITDPWREVSWEEAINRAASEFKRIQQ
                     KYGRGSVGGITSSRCTNEETFLVQKMIRAAFGNNNVDTCARVCHSPTGYGLNTTFGTS
                     AGTQDFDSVNEADVILVIGANPTDAHPVFASRMKHRLREGAKLIVIDPRRIDLVRTPH
                     VEAQYHLALMPGTNVAILNALAHTIVTEDLHNKKFVEDRCDLADFRIWLDFIKRPENS
                     PEALSKTIGVPASEIRAAARMFARGPNSAIYYGLGVTEHSQGSTAVIGMANLAMLTGN
                     IGRNGVGVNPLRGQNNVQGSCDMGSFPHELSGYRHISLDGPRTMFEKDWGVHLDPEPG
                     LRIPNMFDAAIDGRFLGLYVQGEDIVQSDPNTKHVQKALASMECVVVHDLFLNETAAY
                     AHVFLPGSSFLEKDGTFTNAERRISRVRKAIAPMAGLADWEATMALSTALGYPMNYKH
                     PSEIMDEIARLTPTFAGVSYDRIDELGSIQWPCNEKAPEGTPIMHMGGFVRGKGKFEL
                     TEYIPTDERTGARFPLILTTGRILSQYNVGAQTRRTENSVWHEEDMLEIHPHDAEDRG
                     IRDGDRVALMSRSGETSLHARVSERMQPGVVYTTFHHPGSGANVVTTDNTDWATGCPE
                     YKVTAVQVRPTNRWSQWQEDYEEFKAVTMRISDPKVAAE"
     gene            complement(18116..19681)
                     /locus_tag="VGM72_08295"
     CDS             complement(18116..19681)
                     /locus_tag="VGM72_08295"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEY4265303.1"
                     /translation="MTTRIYVPSDSAALSMGAEAVAHAVSAEIVSGKLDAKLVRNGSR
                     GLLWLEPLLEIETPEGRIGYGPVRASDVKAILDSGLTAGTSSDHPLKLGLVEAIPYLA
                     NQQRLTFARCGITDPLSLEDYENHGGLKGLRRALDMAPTDIVKEVTDSGLRGRGGAGF
                     PTGIKWKTVLGCEADQKYIVCNADEGDSGTYSDRMIMEGDPFALIEGMAIAGLAVGAT
                     KGYVYIRSEYPHAFHQMIRAIEIARDKNLLGVMVLGSNRIFDMEARLGAGAYICGEET
                     SLLESLEGKRGQIRFKPPLPAIEGLFGKPTVVNNLISLATVPVILAEGANHYKDFGMG
                     RSRGTMPIQLAGNIKHGGLIERAFGLTLRELIYDYGGGTYSGRPVRAVQVGGPLGAYW
                     PESLFDVPFDYEAFAAKGGMVGHGGIVVFDDTIDLAKQARFAFHFCAIESCGKCTPCR
                     IGSTRGAETVDKIIRGERVVENIDLVRDLCETLRDGSLCALGGLTPMPVMSAMDHFPE
                     DFDRKPTHAVAAE"
     gene            complement(19678..20163)
                     /locus_tag="VGM72_08300"
     CDS             complement(19678..20163)
                     /locus_tag="VGM72_08300"
                     /inference="COORDINATES: protein motif:HMM:NF004638.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEY4265304.1"
                     /translation="MSVRPHSGPVSWDATAAREIAVSLKQLEGPLLPILHAFTEHFGH
                     VSDEAIVLIADVLNLTRAEVQGVVGFYHDFRREPAGRHVVKVCRAEACQSMGSEALAA
                     RLERALGISFDDTTPDRRVTLEEVYCLGNCALSPNVMVDGELCGRADEAKVLALVGTH
                     S"
     gene            complement(20234..20977)
                     /gene="fdhD"
                     /locus_tag="VGM72_08305"
     CDS             complement(20234..20977)
                     /gene="fdhD"
                     /locus_tag="VGM72_08305"
                     /inference="COORDINATES: protein motif:HMM:NF014671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEY4265305.1"
                     /translation="MANEVPVALEFDGSAYAVMMLTPADLEDFAVGFALSERIIARGD
                     EIARIDIRYGSRGIGIDVRLEKEHSARLAQRRRAIPGQSGCGICGLTTIEEALPQLAR
                     LTAAPRLESKVLFEALAALPARQSLNQQTGAVHAASFCRTDGEILAVREDVGRHNAFD
                     KIIGHLAREAIDPASGFSLLTSRCSYELVQKSVLARIPILATISAPTELAVSIAREAN
                     LTLVALARGDSLLLFNDPFNVFAPGSYML"
     gene            21193..22080
                     /locus_tag="VGM72_08310"
     CDS             21193..22080
                     /locus_tag="VGM72_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265306.1"
                     /translation="MSKSIMFATIAAAFVAWPVMAAQTAIPDFSGMWGRNAFNFEPLP
                     RGPAPITNLKRLPNGAGDPNQLVGDYKNPLLRPEAAEAVRQKGEISKTGANYPDPSNH
                     CAPYAPPFTFAMQLGMQVLQANDRITMIYNQDDQVRHVRLNAMHPAHATPSAMGDSVG
                     HYEGDTLVIDTVGIEQGEIPIIDRYGTPRSEALHVIERYRLVDYETAKEAAERHQKKD
                     GPAGLGGNVSVDPDYKGKGLQLQFTVDDPKVFTAPWSARVTYRRTKGDWTEQICAENP
                     FEYYSGKTTAIPMAETPDF"
     gene            22222..23430
                     /locus_tag="VGM72_08315"
     CDS             22222..23430
                     /locus_tag="VGM72_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265307.1"
                     /translation="MTNTKKPKAPKAPKAPKARTKKPAHRTTPEGVVVLVATRKGAWF
                     YRGDKGRKSWQADGPHFLGQIINHLVLDPRDRKTVLAAASTGHLGPTIFRSTDLGRSW
                     QEAKRPPAFAKAPDGEKGRSVKHTFWLAPAHANEPNIWYAGTSPQGLFRSEDGGNNWE
                     PFGTVNDDPQYRAWMGAEQDGTPDGPKMHSILVDPRDPAHLYMAMSGGGVHESHNGGR
                     TFAPLVKGLEVVEGFDAANITFHDPHCVRLCPSDPDRLYQQNHCGIYRLDRPSDEWVR
                     VGRKMPKRVGDIGFPMVVHPRDANTVWVFPMDGRNVWPRTSPDGTPSVYASRNGGKTW
                     QRLDSGLPQSQAYWTVKRQAMTADSADPVGLYFGTTSGDLWMSRDEGEKWTTLARHLP
                     EIYAVEVAEV"
     gene            23427..23684
                     /locus_tag="VGM72_08320"
     CDS             23427..23684
                     /locus_tag="VGM72_08320"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY4265308.1"
                     /translation="MKVLIPTPLRSYTGTREVEADGGTIGALLAELDRRYPGLRFRMI
                     DELDRVRPHMRVFVNGEQTFDLTRALVPADSVQIVQALSGG"
     gene            complement(23671..24282)
                     /gene="mobA"
                     /locus_tag="VGM72_08325"
     CDS             complement(23671..24282)
                     /gene="mobA"
                     /locus_tag="VGM72_08325"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR02665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HEY4265309.1"
                     /translation="MILAGGRSQRMGGVDKAFLTLGGETLIARTIARAKPQVGELLIN
                     ANGDSERFAGFGLPVIPDAVGGFLGPVAGILAALEWMRAKRSQARWLASFSCDCPFFP
                     IDLVDRLIDGAQHENVPVAIAASGARHHPVFAVWSSTITATSQGALVDRGLRKMDDFV
                     ALFLNARVGFASDPIDPFFNINTPYDLAHAETRLANDESFTRR"
     gene            complement(24387..26084)
                     /locus_tag="VGM72_08330"
     CDS             complement(24387..26084)
                     /locus_tag="VGM72_08330"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020057977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEY4265310.1"
                     /translation="MPASQHQNPKSDIEISQAAKMTPIMEIAKKRLGIDPQNLDPYGH
                     YKAKVSMDYIKSLKNKPNGKLILVSAITPTPAGEGKTTTTVGLTDALNHIGKKAMLCL
                     REPSLGPSFGMKGGAAGGGYAQVVPMEDINLHFTGDFHAITSANNLLAALLDNHIYWG
                     NSLNIDQRRIAWRRVLDMNDRALRQIINSLGGVANGFPREDGFDITVASEVMAIFCLA
                     SDLDDLKKRLSNIIVGYTRDRKPVRAGDLKAQGAMTALLKDALSPNLVQTLEGTPAFI
                     HGGPFANIAHGCNSVLATTTALKLADYVVTEAGFGADLGGEKFLDIKCRKAGIEPSCV
                     VLVATIRALKMHGGVKKEDLKKEDLTALESGMSNLKRHVENVRKFGLEPVISINRFSA
                     DSAAEIELVKTACAQLGVECYMADHWAMGGEGAADVARAVVKICDSGKSKLKFLYPDD
                     MPLLEKIRTIAREIYRAKDISADKSVRDQLANFEAMGYGKAPICVAKTQYSFSTNPDS
                     KGAPEGHTINVREVRLSAGAEFVVAICGEIMTMPGLPKTPSADSIDVNADGKIVGLF"
     gene            complement(26111..27040)
                     /locus_tag="VGM72_08335"
     CDS             complement(26111..27040)
                     /locus_tag="VGM72_08335"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY4265311.1"
                     /translation="MRIVVHGQQAFGKAVLDALIARGENVVAVYVAPEKPGAKADPLK
                     DAAVAAGIPVLQPASYRKEEVWEEFKLLAPDLQVMAFVTLFVPEEFLNIPTYGSIQYH
                     PSLLPKGRGASAINWPIINGETETGLSIFWPDNGLDTGDVLLQKKTTISDTDSLGSVY
                     FDRLFPMGVEAMMESIDLVKAGKAPRIKQDENLATYEGLCRADNAKIDWGKCCKQIDQ
                     VIRGCSPAPGAWTMIDGKTVQIFEAKPQPAKTPKDIGGKLGEVVEITADGFSVVCADG
                     RIKVLRVKPADGGKASAADFAKTTNLAQGTRLG"
     gene            complement(27113..28630)
                     /gene="frc"
                     /locus_tag="VGM72_08340"
     CDS             complement(27113..28630)
                     /gene="frc"
                     /locus_tag="VGM72_08340"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HEY4265312.1"
                     /translation="MAKAKAKKAKAAVKKPAPKKSAKKVTAKAAKKQAPKKLVVNKLR
                     VARKAAAKKPAPKRKATMANGNGYPAPSTKPWGQDGKALDGVRILDFTHVQSGPTCTQ
                     LLAYMGADCIKIERPGVGDITRTQLRDVKGADSLYFTMLNANKRSITIDSKNPKGIEI
                     LERLVKTCDVLVENFAPGALDRMGLTWERVHQLNPRIIMASVKGFGPGPYEECKVYEN
                     IAQCAGGAASTTGFREGPPLVTGAQIGDSGTGLHLALGIVSALYQRNRTGRGQRVLCA
                     MQDGVLNLARVKLRDQQRLKHGPLTEYSQYGEGIPFGNAVPRAGNDSGGGQPAWLLKC
                     KGWETDPDAYIYFITQAPVWSEICDLIGKPEWKTDPDYATPPARLPRLKAIFGIIEEW
                     TMTKTKFEVMDICNEHDIPVGPILSMKEIAEEQSLRATGTVVEVDHPVRGKYLTVGNP
                     IKLSDSPCEVERSPLLGEHTVEILTKMLGYTESDLAEIKSSGAITPPEKSRAEAA"
     gene            complement(28668..>29639)
                     /locus_tag="VGM72_08345"
     CDS             complement(28668..>29639)
                     /locus_tag="VGM72_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY4265313.1"
                     /translation="LPMSMGKGLLPDTHPQCAGAARSTVLAQSDVVMLIGARLNWLLS
                     HGKGKTWGTEPKKFIQIDIEPKEMDSNVEIVAPVVGDIGSCVNAMLTAMGSWPAASAD
                     WMKAVTTKRDENVAKMAPRLMNNNVPMDYHGALGVLRTIVKERPDAILVNEGANTLDL
                     ARGIIDVYQPRKRIDVGTWGIMGIGMGYSIAAAIETGKPVLAIEGDSAFGFSGMEVET
                     ICRYNLPVCIVIFNNDGIYRGTDVNKVSSDPAPTMFVKGSRYDKMIEAFGGVGVNATS
                     PDELKRAVNAAMDSGKPTLINAVLDPAAGSESGRIGNLNPQSALKKK"
BASE COUNT         5711 a         8818 c         9080 g         6030 t
ORIGIN      
        1 ttcgacgaac ggcgcattgt ggaactcacc gtgctgatcg gcacctacag catgcaggcc
       61 cgcgtgatgc aggcgctcga aatcgatccc gaacaggcct gagcgcgtta aacttggtta
      121 cgaaagctcg gcaccggccc catctggaac aaatcctcgg tattccgcgt tgtccggagg
      181 cctgctccct gagcacgtat gagaggattc ttatgttcca gctgacgcac aattgggagc
      241 tgccacccat tggcctgagc gtgccgggat gcgtagagga agctattcac caatgcgccc
      301 gtggggaatc ctcgcccgat ctcgccttga tgcagcttct tgtcgcctcg tgctccgagg
      361 aggagaccga acgggtgctg ggaacggcga tatgggacgc ccttgaagac cgcaatgcgg
      421 gaactgcgga gagactcggc gccgttcaga agctttggga tggggctaag gacctcgtgc
      481 aatccgccgc cccgagcctc tgccgctgaa attcacgata atatcttggc catatcgggt
      541 taagggagca accgccccat cggcggcacg ttattcctat ggggcattgt gcatcgagga
      601 aggtcatgga aatagcgacc gaaatcacat tccataatat gagaagttcc gaaggtctta
      661 gagacgacat tcttaagcgc atcgccaagc tcgaaaaatt ctgcggccgg ctgatttcct
      721 gccgggtatc catcgaagcg cggacaaaga atcatcgcac cggcaatatc tacgaggtgc
      781 atatcgagat gcaggcgcca gggaataccc tcgtggtcag ccgcgaaccg catcacgtca
      841 gggaacgtcg cgcgcatagc gatgtgcgga cctccatcat cgaggcattc gcggcggcgg
      901 aatcgcagct gcggtctttc gggaagaaaa agaccggcga ggtaaagacc gcaaaacgag
      961 gagacagcac gcaaacgcta taagataggg cggacgtttc tttcggccgg ggagcggaac
     1021 tgtaccttcg ttcgacatat ggacaattat gaaggcggag attttgacaa ccgggccgac
     1081 gcggggcgca agctcgcgcg cgcattggaa catctgaggc cggaacatcc cgtggtgctg
     1141 gcgctgccgc gcggcggcgt gccggtgggt tttgaggtgg cccgggcgct ccaggccccc
     1201 ctcgatgtgg tgctggtgcg caagatcgga tcccccggcc agccggaatt gggcttgggg
     1261 gcggtggtgg acggcgccaa tccgcagctt gtcctcaatg aggaactggt cggcttggtc
     1321 cagcctggga ggcgctatct cgaagccgaa gaaaagcgcc agctcgcgga aatcgaacgc
     1381 cgccgcgcat tgtatcggcc gggccgcgcg cccatatcgc ttgccggacg catcgtcatc
     1441 gtggtggacg atgggatcgc aaccggtgct acgatgaagg cggttcttca ggccttatcc
     1501 caatccgaac ccaagcgcgt ggtcctggcc gtgcccgtag cagcagccga ttctttggaa
     1561 gagcttgcga aattggccga tgaaacggtg tgcctcatga cccctgcgcc gttctattcg
     1621 gtgggtgcct tttatcgaga ttttacgcag acggcggatc aagacgtgat cgaccttctc
     1681 cggcgcgccg agacattggt gccgaaagag gccgagaccg cagcgcctat tgcttcggat
     1741 gaatgaccgc gacgagctgc acgtcgaaca gcagcgcctg gccccacaaa tccgaattgt
     1801 tgcgctcctc gccataggca agcttggcgg gcactagaag ttcccattcg tcccccgcct
     1861 tcatcagaga gagcgcttcg atccatcccg gaatcacctt ggccacggga aggtcgcggg
     1921 tctcgccggg cttggtggaa tcgaagacat agccatccac gcgcgtgcct ttataggaca
     1981 ccgtgaccgt gtcgttggcg gtaggcgtct cgcccgtgcc ggatttgatg acgcgatatt
     2041 gcaatccgct tggaagcacc atgaccccgg gcattttcgc atgggccgcg acaagttcgc
     2101 gattggcttc ggggctgatg tcgccggcga tcgccggacc tgcgaagccg atgaaaagcg
     2161 tcgccacggc gaatttcatc atccgcatac gatcacctgc acacctccgc gccttcacgg
     2221 ctttgggacc acgatcagct tccaggcccg ttcgtcggtc agatattcgc gggctacgcg
     2281 ctggacatcg gcgggcgtca cggtctcgag accgggacgc gcgtcgcgga tgagcttcag
     2341 ccgccgttca tcggtttgcg ctcccgaaag ggagcccagc caataattgt tggtctgtac
     2401 ggctttttcg agtgtatcca gttccggttt gcgggcacgt tcgaattcgt ccttgctggg
     2461 ctcgcgggcg cgcagatcgg cggtcacctg tttgaccgta tcaaagaaga gctgtgcctt
     2521 ttcaggagga atctccgcat aggcggcgat atagccatac cccggaaagg ttcgtgaggc
     2581 gctggcggtc gccgaaggcg aataggtggc tccaaacttg atgcgcaatt gctccatcag
     2641 ccggaattgc aggatgtccg tcacgacacg ccgggccgcg ctttccgaat tcgcgtatac
     2701 gtccgtggtc tgccatgcga tggccgcaac accttgatcg gcgcgtccgg cgtggtgcag
     2761 cacgatgggt tcgggcgtgg gggcggggaa tttgagatcg cccgccgccg gaagcgccat
     2821 gggagcgcca gaccgccgcg gcaatgcgcc gaaggtcgcg gcgaccgcct ttgtcgcctg
     2881 ttcgaccgag acatcgccca ccatcgtcac ctcgatcggc ccattggcaa gggccggcgc
     2941 gatgatcgcc ttcacctggt cgagcttggc tgtctgcaca tcggccaacg tgggataggc
     3001 ccagcgcgca tcgcccggat gcagcagctc ggcgaatttc gcgccgaaca gggacatcgg
     3061 cgtggcatcg agcttggtga gattgtcggt cagtgagctc aagccctgtt cgaacggttc
     3121 cggccgccag cctggcttgg tgatataggc cgcgagcact tgcatttcgg tgtcgagatc
     3181 ggccgggcgc gtcgcgccgg acagcgagaa cgcatcgtcg tcgacgccga atccaacgct
     3241 ggtgatctta cccgaaagcg tgcgtcgcag atccagatag ctgagtgctt ccaatccgcc
     3301 cgacagatag gcgcccgtgt cgatgacgat ccgatccttt gggaaggcca aatcgccgcc
     3361 ggcgatgtcg acactcacca ggatctggtc ggcgcggtat ttggtcggtt tcacggtcag
     3421 gcgcacgcca ttctcgaagc ggatgagagt cgcgtcgaga tcatcgatat ggcgggtttc
     3481 caccacctgt ccggctacgc caaaactggt gtaaggccac accgcgagct gcagctgtgg
     3541 ggcgttttcg attttctccg actccgcctt gttccacacc gaggcaagcg ccgcttcgcc
     3601 gccttccacc ggtttcgggc tggaaaggaa cagtagcggc ccattgccgg cgaacgcgcc
     3661 gcgaagggcc gtattcacct gctcgatggt caggcccttg agtgcgtcct gtatggtcgc
     3721 aagatcggtc gcatcgctgg taaataccga atcggtgtcg acggaggaga ggatgccgtt
     3781 ggcgattccc cgcgacgccc gggtggaagc cgatgccacg gccgattgcg cgcccgtcag
     3841 catgttcgtg atctgccggt ccagctcctg ctgggttacc ccttgtgcca gcacttgccg
     3901 ccggatcttg tcggcttcct cgaaggcgcg ctgccatttg tccgctgcgt agctgacccg
     3961 caaggaagcg attcttgccg aacgcgacac attcccgcgc gaggcgcccg ccgcaagaaa
     4021 gggtgcgtcc ggactttgcg ctgcctgcgc cagccgctga ttgaggatgc cgaagcctat
     4081 gcgttcgatc agatcgcggg tttcatgggc cttggtatcc ggcgcgccgt cataggggcc
     4141 agtccaagct gcggacaccg attgcggtgc gctggtttcg acgaagactt tggtctcctg
     4201 cccgcgcgtc aacggcgtcc cgaggttagg atcgctccgg ccagcgcccg cctgtttcca
     4261 atcggagaag cgtgtcttga tctccttttc gatgacatcg ggatcgatgt cgccaacgat
     4321 caccaatgtc gcccgttcag gccggtaata ggcgtggtag aagtccgcga tttgggacac
     4381 cggcgcggtt ttgatgatgt cgaccttgcc gatgggactg cgctcgggcg ccaattgacc
     4441 tttcaagaga taggcgttct gggcttcgaa cgcgcgcatg ccgggcgtgt cgcgcagccg
     4501 ttcctcggaa agaaccggcc cacgttccgc atccatcgct tcaggcttca ggctcaactc
     4561 ggtcgcgata tcacgcatca gcgtgaaggc ggtatcgagc gtcgttttat cggatgccgg
     4621 gacatcgaac tgatagatgg tttccgtctg gccggtactg gcattggcat cggccccgac
     4681 gcgaagaccg atccgttgca gcatctggaa cacgtcgcct tctggaacat gggtcgagcc
     4741 gcggaaggcc atatgctcca ggaaatgggc caggccgcgc tgggcgtcgc tttccatcaa
     4801 ggagcccgcg ccgatccgca gccggaacga gacctcaccc ttgggggtcg cgttcttctg
     4861 gatcgcgtag cgcatgccgt tcggcagggc gccaaacctc acatcggaat cggccggcag
     4921 gtcgctcttg gcctgtggcc aggcagccgc cgcactgtcc gccgcagtgg cgagccgggc
     4981 cgggccggac agggatatga gaagagcggc aagcgccacc cgcgacagaa gtcccatcgt
     5041 acgcatcaga cctcaccttg taggaagggt ggaatatacg gggtactgtg ggttctcgcc
     5101 attcacacgg acgagatttt gggtcaatcg cgcccgccgg gctcatgcta ggcttgagcc
     5161 tggcaaataa acaagaagca aatgtaatac atggagcatg ccgtggcgga cgaatgcctg
     5221 aatcgcgtct tggtggatgc gccgacaatg acgcgaggca gctagatgtc catcgaagac
     5281 ggacttcgct ttcgcatcgc acgcgcctgc cgtgtgttgg gaaagatgga gctgacccgc
     5341 gcgttgcgcg ggcatatcag cgcccgcatc cccggaacgg accgcatctt cattcgtgcg
     5401 cgcgggccgg ccgaaagcgg cgtgcgctac accaccgagg acgaagtggt ggaaatcggt
     5461 cttgatggca aacccgtagc gggcatgccg cagggccttc ggaccccgag cgaagtcttc
     5521 attcataccg agatcttccg cgcccggccg cgggtcaacg ccgtgctgca tctgcacccg
     5581 ccaattgtcg tgctgttcac ggttaccggc gtgccgttgc tgccgatctg tggcgcctac
     5641 gatccgcaag gactgggact ggcgctggaa gagcccccga ccttcgagtc gagcgtgctg
     5701 atcgacacgc ccgaattggg ccgggaattg tcggcggtta tcggggacaa gcgcgcctgc
     5761 ctgatgcgcg gtcacggcat cacgacggcg gccgattcca tcgaggaagc ggctgtcgtc
     5821 gcactgtcgt tgaatgagct ggcaacgatg aattaccagg cccgtgcctt gggcacgccg
     5881 cgtccgattt cagaaagcga ccgcgaggcg ctcagccctg ccatgaaacg gctgatgaag
     5941 cgcggcccgc ggcccggcga gccaggcgat tccgtcgtca ccgaatggcg ctattattgc
     6001 cgtctcacgg gagaggacgg cggcacctga tgatgcaacg cgatattgtc cctattggac
     6061 cattgtcgcg gtctgacata ataatcgcgc acgcgtcgtt acgaaccagc ccggccgttg
     6121 agtgagaacc ccatgcgtct gaaaactctg ctcatctggt tgctcgcttg cgctgccggg
     6181 cccgcatgcg ccgatgaggg catgtggacg ttcgacaatt tccccgccgc tcaggtgaaa
     6241 cagaaatatg gcgtcacgat cgaccagaaa tggctcgatc gtgtgcaggc gagcgcggtc
     6301 cgccttgcgg gcggctgctc ggcctccgtg gtgtcgccga atgggctggt gctgaccaat
     6361 catcattgcg tggccggctg cgcacagaat ctgtccacgc ccgagcacga ttacgtgaag
     6421 aacggctttg ccgccgcgcg agccgaggac gaaaagcagt gtcccggtgt gcaggcggaa
     6481 atcctgcaaa ccatcgcgga tgtgacccct gccatcaacg ccgccactgc ggacaaggcc
     6541 ggcgatggtt ttcagcgggc gcgcgatgcc gccgtcgcga atatcgaaaa acaggcatgc
     6601 accgggcgcg aggcgacgca gcgctgccag gtgatcacgc tttacgacgg cggccagtat
     6661 aagctctaca cctaccgcaa atattccgac gtgcgtctgg tcttcgccgt cgagttcgac
     6721 accgcgttct tcggcggcga ccccgacaat ttcaattttc cacgctacga cctcgatttt
     6781 tcgttcctgc gactctatga gaacggcaag acggtcgcga cgccggaaca tctcacgtgg
     6841 aacccgtcgg ctcccaagga tggcgagcct gtcttcgtcg ccggcaatcc cggcggcacg
     6901 gaccggcaat tgacggaaag tcagcttgtc acgttacgcg attccacgca gcctttcggt
     6961 ctgatcctgg gttcggaact gcgcgggcgc tacacgcgtt tcgccgaaga aagcgccgag
     7021 catgcccgca tcatcaatcg cgatctcttc ggactcgaaa acgggctaaa ggcgcagcgc
     7081 ggcgaacatg aggcgctgct gaacccgcaa ttgatggccg acaagcacaa ggccgatgcc
     7141 gaattgaaat cgcgcgtcga tgccgatcca aagctgaagg cggagatcgg cgatccttgg
     7201 gcggagatcg caaaggcgca ggccgaacgc gtgccccttg cgaagcccta ttattttatc
     7261 gaaacgcgtg ccgggggcgg ctcgacactc ttcggctacg cgcgaatcct ggttcgtgca
     7321 gcggacgagc gcaccaagcc caatggcgaa cgcctgcgcg aatatgccga tgcgcgcctg
     7381 ccgctgattg agcggggcct gctcgccgcg cgcccggtgg agcccgaggt ggagcagttg
     7441 aagctcgaat tctggctcac caagctgcgc gaggaattga ccgccgacgc acccgaaaca
     7501 gccctggtcc tcggcaagga ctcgccggaa aatctgtcgc gcgctttgat cgcgtccaag
     7561 ctcgccgatc cgagcctgcg caagcaattg tgggagggcg gcgccgcggc ggttcgggct
     7621 tcgaccgatc cgatgatccg gtttgcgttg aagatcgacg ccgcgggtcg cgccatccgc
     7681 aagacctatg aggagcgggt gtcgagcccg gtcgctgccg cgtcggagcg tatcgccaag
     7741 gcccgcttcg ccgtctatgg cacgagcgtc tatcccgatg cgaccttctc gctgcggctt
     7801 tcttacggga agattgcagg ctggacagag cccggccgta cgattctgcc ctttaccaac
     7861 tttgcgggtt tgtatgagcg cgcgacgggg cagccgccat tcgaactcac cccgcgttgg
     7921 gtcgaggcac aatccaagct cgatctcaag acggtgttca acatgtcctc ggacaacgac
     7981 atcatcggcg gcaattccgg ctcgcctttg atcgatgcca agggcgaggt gattggcgcc
     8041 attttcgacg gcaatatcca ttcgctgggg ggcgattatt atttcgatcc caagctcaat
     8101 cgttcggtca cggtgtccac cgccgccata accgaggcgc tgaataaggt ttacggccaa
     8161 accggcctga tctcggaatt gacgggacat tgatttttcg ccaaggacag agtgtgggaa
     8221 taccagtaag gttccctcgc cgatgaaggg tgcatgtcca atgaattcca tgcgtaagtg
     8281 catcgccgct ttggggtttg ccatgctgcc cacgcttggg gcgtggccgg cgatcgctca
     8341 aacccccgcg aagattcctt acccgcagaa agtggtgatc ctcgtcaccc attcgagtcc
     8401 gggcgcgggc agtgacgttt tcctgcgcga gctggtgcgg tatctgcaga aatacatcaa
     8461 tgccaccttc gtcgtggaaa acgacgaggg cggttccggc gccaaggcgg tttcacgtgt
     8521 cgccactgcc aagcccgacg gcagcatgct ctatgccgcg accccgacct atatattgac
     8581 ctcgctgctc agccacccga gcaacaccta tcgcgatctg gaaccggtgg tgaatttctt
     8641 caccgactcg gaaatcatct acgttcgcgc cgaaagccct tacaaatcgc tgaaggatct
     8701 gatcgatcac gcgagaactt cgcgtgggcg ctggggcgcg gccaatcccg gatcgctgga
     8761 acgccaggcc gctgaacaac tgaagatcgc ggcaaaggtg aatgccgccg ttgtcagcca
     8821 cgagggtggc ggcgacatga tgatcaacgt gctgaatggc acgctcgaca tgggcgtggg
     8881 cgagatggag gaaatccgcg cccaatatga agcccataag gtgcgggtgc tcgcgacgtt
     8941 caacggcacc cggatgcccg cacttcccga tgtgccgacg gtcaaggaat cgggctacaa
     9001 cgtggtcctg gagaagtttc gcggtctcgc ggcgcccaaa ggcctgcctc cggccattgc
     9061 caagatctgg gacgatgccg cacagaaaat cctcgccgat ccggaatatc gcaaggccta
     9121 tctggaagat gatttgatcc cccattatct cgcgcacgac caatatggtc ccttcatcac
     9181 gaagtttgcg gccgacaccg gtgcattcct gaaatccacc ggcgtcattc gttgatgcgc
     9241 aaagacctcg tcgccgctgc gattctgttg tgcatcgccg cggcgtatta cgcggcctcc
     9301 acgacaattc cctccagcac gctggaagat tcggtggggc cgcgcggcct gcctgtcgtg
     9361 ttggcggttc ttcttgcgat ggtcgcaatt gtgatcgggg tgcgcgcgct cgtcaccgcg
     9421 ccggtttcgg gcgaagcggc caaggatgcg gaagcgccct ggccgcgcgc catcggcatg
     9481 ctggtgatcg gcgcgcttta tattccggtg gcgaactttc tcggttattg gctgacattg
     9541 gtcatgctgt tgctcgcggt tatcctgtac gaaggcatga aaccgtcctg gcgcatgctc
     9601 gccgttgctt tggggggcgc tacgttcttc tttcttttgt tcgatgtcct gctcggcgtt
     9661 cgcgtgcccg aaggcatgtt gttctgagat tcgggcgagg gggaggaaca tggaagagtt
     9721 cagacacgcg ctagagatgc tcgtcaccac gtctgtggtg cccgcagccc ttggcggttt
     9781 gatctggggc atgctgggcg gcgcgacgcc gggactctcg gcctcgatca cgatggcgct
     9841 acttttgccc ttcacctata cgctgtcgcc gatgcccgcg attgtgatgc tggcgacgtg
     9901 ctacatcggc gccgaatatg gcggttcgat tccggcgatc ctgatccgga cacccggcac
     9961 caatgcttcg gccgcgacga tgctggacgg ttatgagatg aaccggcaag gtcgcgcagg
    10021 cgaagcgctc ggtctgtcgc tgtggtccgg tgtgattggc ggtctgttcg gcctgaccat
    10081 gttggtgctg ctcaccgagc ctttgtcgcg cctcgcattg ctgtttaggc cgacgaccta
    10141 tttcggcctc ggcattctgg ggctttcggt gattgcctcg ctatcgagca aatcgctgct
    10201 caagggcatg ggcgcagcac ttttggggct gatggtggcg accatcggca ccgatccggt
    10261 ttcgggtgta agccgcttca cgtttggtgt gcccgatctt ctggacggca ttcagcctct
    10321 tttcgtcatg gtggggcttt acgcagtcac cgaattgatg acgcgggccg gcatcacgcc
    10381 caacctgatt aaatcgaatg cttcgatccg cgtacgtttt ccgcgctttc ggatgatgaa
    10441 aaaggtcgca cgttcccaga tcatcggctg cgccgtcggc acgttcgagg gtttgatgcc
    10501 gggtgctggt ggcacgattg cggcatttat cgcatatgat caggcaaagc tctggtcgaa
    10561 acatcccgaa gaattcggca agggcgccga ggagggtgtc gccggtcccg aagctgcaaa
    10621 taatacggtt gccagcacag cgctaatccc gcttctcagt ctggggattc ccgcatccaa
    10681 ttccgcggcg gtcttgctcg gaggattgct gatccacggt ctgctgccgg ggccacgact
    10741 cttcgaacag aattcggatg tgatcctcgg gctctatacc ggcacctttg tggcggacat
    10801 cgcacaggtc atcatcggga tcatggtcct tcctgtttgt gtttggttgg tgaatcgtcc
    10861 gaagccctat cgctcggcat tcatcctcgc gctcgtgctc tcgggcgttt acacggtgca
    10921 tatgtcgctg ttcgatgtcg gtatcgtgtt gtccgccgga ttgctcggtt atttcatgaa
    10981 atatttcggg tttcccttct tgcccgcagt ccttggcgtc gtgttgggaa cgaagctcat
    11041 cgaatccaat tatcgccgtt cgctggtcct ttccggcggc gatttcgaca tcttcttcca
    11101 agatccgatt gcggtcggat tcttcgtggt ggcagccatc attatgctcg gagcgcttta
    11161 cgcggaatgg cgcgcggcgc aacaacgaaa agtgagtttg gcgtgacgat ttcacaacag
    11221 cttgcggcat tcgccgctgc gacggcgact gcacaactac cgcagcgggc acgcgaaaca
    11281 atccgtctcc ttctgctcga tgttgcgggg ctctgtgtcg cggcgagaag taatgattat
    11341 gtggccgctg cgcgcggcag cgcggtgtct tccggatgca cgacagcgat cggccacgcg
    11401 ggcacgttcg gcccatatga cgcggcgctc attaacggca cggctgctca tggcgaggat
    11461 tacgacgata cgtttgaagg cgggcctgtc catgctggtg cggtgattgt tcccgcagta
    11521 ctggcggcgg ccgagcatcg tggactttgc ggtgatgcgg tggtgaaggg aatcgctgcg
    11581 ggcgtggaag tgatgtgccg catgagcttg gtcgcaccac aggcgacgca caaagcctgt
    11641 tttcacccca ccgccatctt cggtgcgccg gcggcagcgg ccggagtggc ggcggcgctc
    11701 ggattgccgc cagaaaaaat agcggcggca atcggagtag cgggaagcct cgcgtcgggc
    11761 atcatcgaat atctcgccga tggaagctgg accaaacggc tgcatgcagg ggccgccgcg
    11821 caatcgggga ttcgcgcagc gttcctcgca gagggtggat tcaccgggcc tttgacggtg
    11881 ctcgaaggcg gtcacggatt ctacaaagca ttcgccccat cgaaagtccc ggattttcaa
    11941 ccgctgctga atcgtcttgg tacggagtgg gtgctcgaaa ctgtcgcctt caagccctat
    12001 gcgtgcggaa ccatgacgca gcccttcatt gactgcgcga ttgaacttgc gcggtccggc
    12061 gtacacgccg aagacatcgt cagtctggtg tgcgaggtgg gcgaaggtac cgtgcatcgc
    12121 ctatgggaac cgcttctgca gaagcaggcg ccccccaacg gctacgccgc gaaattctcc
    12181 acgccctatt gcatcgccgc cggattcgtc gacggcaagg cgggcttcga gcaattcacc
    12241 gatgcgcgcg tcgccgatcc gaaattgtgt gcgctcgcgg gcaaggcctc ctacgtgatc
    12301 aatcccgacg atccctatcc gaagaatttt accgggcata tccgcgcgac actgagggac
    12361 ggcacagtgc gtgaagtgcg caaggcccat atgcgcggcg gtgcacacga gccgctatct
    12421 gaagcggaca tcatgaccaa gttctatgac aatacgcgct ttggcggctg gccgcgcgcc
    12481 aaagcggccg ctttggccga agccctcgac cgcattgtgg ccgcaggctc cgccgccgcc
    12541 gatctcagca cagcaaggga ataagatgca ggaacttgaa ggttcggttg ccatcgtcac
    12601 aggggctgcg cgcaatatcg gacgcgctat cgcgcttgat ctggctgatg cgggcgcagc
    12661 cgtcaccgtg ctggcgcgct ccgatattgc cggggtgaaa tcggtggcgg ccgaaatcga
    12721 agggcgtggc ggccgcgcgc tggcgatcca gggcgacgtg actcagcctg acgatgtgcg
    12781 gcgtgcggtg gacgagaccg tgaaaaaatt cggcaggctc gacattctcg tcaacaatgc
    12841 gggtgtacgg ccggaaggcg cattcgagga tctgagcctg gattcatggc atcaggtatt
    12901 ggcggtaatc ctcgacggtc cttttctgtg tgcccaagcg gctctgccgg ccctcgaaaa
    12961 aagcggccgt ggcaccatca tcaatattgg tgggcttacc gcctatacgg gtgcggcgca
    13021 tcgcgcacat gtgattacgg cgaaggcggg actcgatggt ttcaccaagg cactggccgt
    13081 cgagttggcg cctaagggta ttaccgtcaa tcttgtggcg cccggcatga tcgagacaaa
    13141 acgcgagggt gccgaacccg tgcatcgcaa gacccgcacg attctggcac ctcggcgcgg
    13201 ccaaccggaa gacatctcgg ctatggttcg ccatcttgcg ggtccgaggg cgcggtccat
    13261 gaccggccag accatacaca tcaatggcgg cgtctatctg ccgtaacgag cctatccgtt
    13321 caggtaccag atggagaaat tcagaactgc ggcgaatgca acccaggccg cgagtggcac
    13381 aagacaccat gcggccagcc gatccaactt ccagaaagcg atgatggtca tcacgatgat
    13441 cgtgagttgc ggtaggatat cgatcaatcc aaggaccggg ctgtgagcag cgaaaaacat
    13501 ccacgaccac gcggcgttca gcgcgagctg gatgtaaaat aggatcaaag cgttatgatg
    13561 ctgcggcgaa gtgggcaacc gcaaaactcg ccagagcgca aaccccatca gcacgaatag
    13621 tgccgtccag accggcccaa atatccaatt gggcggattg aacggcggct tttccagacc
    13681 ggcataccaa atcgccaagt tggggaaggt cgcaatctgg ccggctatcg atgctgcggc
    13741 gactgaagca aaggcaagga tcgccaagcg caaatcggtt gacggggaaa gccctgcgcg
    13801 ccgtaggctc atgttctcgc tctcggcatc acctcacaca aacgcgcaac caccagttcg
    13861 gggtccgcgt ccgtgcgagg ataatgtctt aagcggcggc ggctttcttg cgattggctt
    13921 cgaggcgcga atccacgagc gccacagttg catcgagcgt cgggtgcgag agccccttct
    13981 gcttggcgat caactgcacc agcttgtcgg cgcgctcgat gttcggagcg ccgttctgga
    14041 gagcgcgcgc ggccgaagcg gggcgcgtca gactttcggc ggcggccgca tatttctcga
    14101 acggcacgag gtcgctcggg ttcgcgccca acttgacgca cagatcgttg acgaaattgt
    14161 agaccgagcg cgaggcttca atgtcggtat gcacggcttc cttggcggtg cgcatgccgt
    14221 ccgcggtcac ggtacggtaa ttacccgcgc acagcattgc ccatttggcg agcggcacga
    14281 agatcgaatc atacacgcgc aatttcaccg gcagttcgat cttgccctcc ggcgtatcga
    14341 atcgcgcggc gtccacgtct ttctccaatt ggcgcaaaat cgcggtgctc ttttcatcgt
    14401 cgaagcgcgc gaccttgaaa ttggtcggca acgtcacttg cagcacgttg gccttctcct
    14461 ccggcgggcg gatggcctgc gggtcagggc tgttcaacgt caagcatgcc ggatcgaaat
    14521 tgtcccacac ggtcggatcg gtgtaggcgg gtttcagcgc ctcatagttc aatccgggaa
    14581 tgcgcttcac atagggcagc ggcggcatat tcatgatcga catgcagggc acgcgcgatc
    14641 tggcgaccgc atccagaagc tcgcgcacgc cgggcgaacg atattgcggt tcctgcatcg
    14701 ccaggccgac gagatcgtag tctttcggat tcacgccggc ggccggaccc gccgtcacct
    14761 tgccaggaag attgcgcgac ttgaggatca cgggatcctt gcgcccgcgg atgggcaacc
    14821 ggacctcgaa accttctgca ttgatcagat cggcttccgt cggcagacag atgagatgaa
    14881 tggaatggcc accgaagagc agcttggatg ccagaagtga gccataggat gcgccgagaa
    14941 gcaggatttt ataagccatg agaaactccc tagaaattca taatcaatga gtgccggcag
    15001 ccgtaccgga cgccttgcaa acttgttgaa ccgcttttag cgcgatcagg ttcagtcctt
    15061 cgccgccatg tgccagatgt tccgccatct tggcccgcat gtgtggatcc cagaatttcc
    15121 tgatgtgatc ggccgcgccg gcgactgcct gatcttcggg tttcaaacgg aagaaatcgg
    15181 cgatctggtt cgccatccgt acaagattat tgtcgtgcat gtgctactcg gccgccactt
    15241 tgggatcgct gatccgcatc gtcacggcct tgaattcctc atagtcttcc tgccattgcg
    15301 accagcgatt tgtcggccgc acctgaaccg ccgtcacttt gtattccgga cacccggtcg
    15361 cccaatcggt gttatcggtc gtcaccacat tggcgccaga tcccggatga tggaatgtcg
    15421 tataaacgac acctggctgc atgcgctccg aaacacgcgc gtgtaacgat gtttcgcccg
    15481 aacggctcat cagtgcgacg cggtcgccgt cgcgtatgcc gcgatcttcg gcgtcgtgtg
    15541 gatgaatttc gagcatgtct tcctcgtgcc agacgctgtt ttccgtccgg cgtgtctggg
    15601 cgccgacatt gtattgagac aggattcggc ctgtggtgag gatcagcggg aagcgcgcgc
    15661 cggtacgctc atcggtcggg atatactcgg tcagttcgaa tttaccctta ccgcgcacaa
    15721 agccgcccat atgcatgatt ggtgtgccct ccggtgcttt ttcgttgcag ggccattgga
    15781 tgctgccgag ttcgtcgatg cggtcatagg acaccccggc aaaggtcggc gtcagccgtg
    15841 cgatctcgtc catgatctcg gacggatgct tgtaattcat cgggtaacca agcgctgtag
    15901 agagggccat cgttgcttcc caatcggcaa gccctgccat cggcgcgatc gccttgcgga
    15961 cgcggctgat gcgccgttcg gcattagtga aggtgccgtc tttttcgaga aaagaagaac
    16021 caggcaggaa gacatgcgca taggccgcag tctcgttcag gaaaagatcg tgcaccacga
    16081 cacattccat cgaagcaagc gccttctgga catgcttcgt gttcgggtcg gactgaacga
    16141 tgtcttcgcc ctgcacgtaa aggcccaaaa acctgccatc aatggcagca tcgaacatat
    16201 tggggatacg cagacccggc tcggggtcga gatgaacgcc ccaatccttc tcgaacatgg
    16261 tgcgcggccc atccagcgaa atgtgccggt agccggagag ttcgtgcggg aacgacccca
    16321 tgtcgcatga gccttgcacg ttgttctggc cgcgcagcgg attcacgcct acgccattgc
    16381 gcccgatgtt gccagtcagc atggcgaggt tggccatgcc gatgacggcg gtcgaaccct
    16441 ggctatgttc ggtgacacca agcccataat agatcgctga gttcggcccc ctggcaaaca
    16501 tacgggcggc ggcgcgtatc tcggaggccg gtacgccgat ggtcttggac aacgcttcgg
    16561 gcgaattctc cggccgcttg ataaagtcga gccagatccg aaaatcggca agatcacagc
    16621 ggtcctcgac gaatttctta ttgtggaggt cttccgtcac gatggtatgc gcgagcgcgt
    16681 tgagtatcgc aacattggtg cccggcatga gcgccaggtg atactgcgcc tcgacatgag
    16741 gcgtacgcac gaggtcgatg cgccgcggat cgatcacgat aagcttcgca ccttcgcgca
    16801 agcggtgctt catgcgcgat gcaaagaccg gatgggcgtc ggtcggattg gcgcctatta
    16861 ccaggatgac gtcggcttcg tttacggagt cgaaatcctg cgtgccggcc gatgtgccga
    16921 acgttgtatt cagaccgtaa cccgtcggcg aatgacagac gcgtgcgcag gtatcgacat
    16981 tgttgttgcc gaaggcggcg cgaatcatct tctgaacgag gaaagtttcc tcattcgtgc
    17041 agcgtgagct ggtgatgccg ccgaccgaac cacgtccata tttctgctgg atgcgcttga
    17101 actcggaagc cgcgcggttg atcgcttctt cccagcttac ttcacgccag ggatcggtga
    17161 tcttctcgcg gatcatcggt ttggtgatgc gctccttgtg ggtggcatag ccccaggcaa
    17221 agcgcccttt gacgcaagaa tggccgtgat tggccttacc gtccttatag ggcgtcatac
    17281 gtacgacgcg gtcgccctgc agttcggcct cgaacgaaca accgacgccg caataggcgc
    17341 aagttgtgat gacggagcgc tccggcgtgc cgtgctggat gacggtcttt tccatcagcg
    17401 tcgctgtcgg gcaggcttgc acgcaagcgc cacacgagac gcattccgaa tccaggaaat
    17461 cctcgttcat tccggcggag acgtgcgaag caaaaccgcg ctcatcgatg gtcagcgcga
    17521 aggtgccctg gacctcttcg caggcgcgca cacagcgcga gcagacgatg catttggacg
    17581 gatcgaaggt gaaataggga ttggactcat ccttcgtctg gcccaggtga ttttcgccgt
    17641 catagccgta gcgcacttcg cgtaagccta ccgcgcccgc catgtcctgc agctcgcaat
    17701 cgccgttcgc cgcgcaggtc agacagtcca gcggatgatc gctgatatag agttccatca
    17761 cgccgcggcg gagcttggcg agcttgtcgg tctgggtcga aaccttcatg ccgttctcgg
    17821 ccggtgtggt gcaagaggcc ggcgtgcctc tgcgtccagc aatctccacc aggcagaggc
    17881 ggcaagagcc gaacggctcc aggctgtccg tggcacagag tttgggcacc tgaatgccag
    17941 cgagcgagga cgcccgcatg atggacgtgc cagcgggaac cgtgacggaa atgccatcga
    18001 tttccagcgt tacggattgg gcggcagcct tctcgggcgt gccccggtcg atggggctgc
    18061 ggtcgatgtc agtatgaacg gtcataacgg gcctcttacg ctttagcgta ttgtatcact
    18121 cggcagccac ggcgtgcgtg ggtttccggt cgaaatcttc gggaaaatga tccatcgcgc
    18181 tcatcaccgg catgggggtg aggccgccga gagcgcataa cgagccatcc cgcaaggttt
    18241 cgcatagatc gcgcaccaga tcgatgttct ccaccactcg ctcgccacgg atgatcttat
    18301 cgacggtttc cgccccgcgg gtcgagccga tgcggcaggg tgtacatttg ccgcaggatt
    18361 caatggcgca aaaatggaag gcaaaacgcg cctgcttcgc gaggtcgatg gtatcgtcga
    18421 acaccacgat gccgccatgt ccgaccatgc cgcccttggc tgcaaaggcc tcatagtcga
    18481 acggcacatc gaacaggctt tccggccaat aggcacctaa cggtccacct acctgtacgg
    18541 cgcgcaccgg gcggccggaa taggttccgc caccataatc atagataagc tcgcgcaagg
    18601 tgagtccgaa cgcgcgctcg atcaatccgc catgcttgat gttaccggca agctggatcg
    18661 gcatcgttcc gcgcgaacgg cccatgccga aatctttgta gtggtttgcc ccttcggcaa
    18721 ggatcaccgg cacggtcgca agcgagatca gattgttgac gacggtcggc ttgccgaaca
    18781 ggccctcgat cgcaggcagt gggggcttga aacggatttg gccgcgcttg ccctccaggc
    18841 tttccagcag cgaggtttct tcgccgcaaa tataggcgcc ggccccgagg cgggcttcca
    18901 tgtcgaagat gcggttgctg ccgaggacca tgacgccaag cagattcttg tcgcgcgcga
    18961 tttcgatggc gcggatcatc tggtggaaag catgcggata ctcggaacgg atatagacgt
    19021 aacccttggt cgcgcccact gcgaggccgg caatcgccat gccttcgatc agcgcgaagg
    19081 gatcgccttc catgatcatg cggtcggaat aggtgccgct gtccccttca tccgcgttac
    19141 agacgatgta tttctggtcg gcctcgcatc ccagcaccgt cttccatttg atgccggtcg
    19201 ggaagcctgc gccaccgcgg ccccgcaagc ccgaatcggt gacttcctta acgatgtcgg
    19261 tgggcgccat atccagcgca cggcggagac ccttcagccc gccatgattt tcatagtctt
    19321 ccagtgataa gggatcggtg atcccgcaac gtgcgaaggt cagccgctgc tgatttgcaa
    19381 gataaggaat ggcctcgacg aggccgagct tcagtggatg atcgcttgaa gtgcccgcag
    19441 tgaggccgga atccaggatc gccttcacat cggaggctct caccggacca taaccaatgc
    19501 gcccttcggg cgtttcaatt tcgagcaatg gttccagcca aagcaatccg cgcgatccgt
    19561 tgcgcacgag tttggcgtcg agcttgcccg aaacaatttc ggcgcttacc gcatgtgcta
    19621 ccgcttcggc acccattgag agcgcggcgg aatcggatgg aacgtagatg cgcgtggtca
    19681 tgagtgcgtt cccaccagcg caagcacctt ggcctcatcg gcgcgtccgc acaattcgcc
    19741 atcgaccatc acattcggcg acagcgcgca attgcccagg cagtagacct cttccagagt
    19801 cacgcggcgg tcaggcgtcg tgtcatcaaa cgaaatgcca agcgcgcgtt ctagccgtgc
    19861 cgccaaagcc tcgcttccca tgctctgaca ggcctctgcg cggcagactt tgacgacatg
    19921 ccggccggcc ggttcgcggc gaaaatcatg ataaaatccc accacgcctt gaacttcggc
    19981 gcgcgtaagg ttgagcacgt cggcaatcag aacgatggct tcgtcgctga catgcccgaa
    20041 atgttccgtg aaggcatgga ggatgggcag aagtggcccc tccagctgct ttaaactgac
    20101 cgcaatttcg cgggcggctg tggcgtccca tgaaacaggt ccggaatgcg gtctaaccga
    20161 caatagatgc tcctgaaatt gcactgtatt cgccttcata taaggccaaa gttcaggaca
    20221 tttaatatga actctagagc atgtatgatc caggtgcgaa aacgttaaat ggatcgttga
    20281 ataataggag cgaatcgcca cgggcgagcg ccaccagcgt caaattcgcc tcacgggcaa
    20341 ttgacacagc aagctcggtc ggggccgaaa tggtggccaa aatgggaatg cgtgcgagta
    20401 cggatttctg caccagttca tagctgcacc ggctggtcaa aagggaaaaa cccgatgcag
    20461 gatcaatggc ttcgcgtgcc agatgcccaa tgattttgtc gaaagcattg tgccgcccga
    20521 cgtcctcgcg caccgccagg atctcgccat ccgtacggca gaaggaggcg gcatgaaccg
    20581 cgccggtctg ctggttcagt gattggcggg ctgggagcgc tgccagcgcc tcgaaaagta
    20641 ccttcgattc aagcctgggc gcggcagtca gtcgcgccag ctgaggcagt gcttcctcga
    20701 tggttgtcag accgcatatg ccgcaccccg actgaccggg aatggcccgg cgacgctggg
    20761 cgaggcgggc tgagtgctcc ttttccagcc gcacatcgat gccgatcccg cgcgaaccat
    20821 aacggatgtc gatccgggcg atttcgtcgc cccgcgcgat gatacgttcg gacagggcaa
    20881 acccgacggc gaaatcttcg aggtccgccg gggtcaacat catcaccgcg taagcgctgc
    20941 cgtcgaattc gagcgcaacc gggacctcat tcgccaaagt gcgggttccg ggctgggaat
    21001 cctcaggcgt gatgcggacc gtgcgatgct ccgcagcggt cttgtattcg ttcggcgaag
    21061 gaaggccgcg ttcgggctca gccacatcga ttttgcgggc ggacatgggg tgattctacc
    21121 ggatcggatg gctcgcgcca aacggtatgg ggtagtattt tcccatctac ggtgaggact
    21181 gcggggaaca tcatgtccaa atcaatcatg tttgccacaa tcgcggcggc gttcgtggct
    21241 tggccggtga tggccgcgca aaccgcgatt cccgattttt ccggcatgtg gggccgcaac
    21301 gccttcaatt tcgagccgct tccccgtggg cccgcgccca tcaccaatct caagcgtctt
    21361 cctaatggtg cgggcgatcc caatcagctt gttggcgact acaagaatcc gcttctgagg
    21421 ccggaggccg ccgaagccgt gcgccagaaa ggcgagattt cgaagacagg cgccaattat
    21481 cccgatccct ccaatcattg tgcgccctat gcgccgccct tcaccttcgc catgcaattg
    21541 gggatgcagg tcctgcaggc caatgaccgc atcaccatga tctacaatca ggacgatcag
    21601 gtccgccatg tacgcctgaa cgccatgcat ccggcgcatg cgacgccgtc ggcgatgggg
    21661 gattcggtcg gccactacga aggcgacacg ctggtgatcg acacggtggg tatcgagcag
    21721 ggagagattc ccatcatcga ccgctatggc acgccgcgca gtgaggcgct gcacgtcatc
    21781 gagcgttacc ggctcgttga ctacgaaaca gccaaggaag ctgccgagcg gcaccagaaa
    21841 aaagacgggc cagccggact gggcggcaat gtctcagtcg atcccgatta caagggcaag
    21901 ggactgcaac tccaattcac cgtggatgat cccaaggttt tcacggcgcc ctggtcggcg
    21961 agggtgacct atcggcgcac aaaaggggat tggaccgaac agatctgcgc cgagaatcca
    22021 ttcgaatatt attccggaaa aactacggcg atccccatgg cggaaacgcc cgatttctga
    22081 tccggctgga gccacaattg tttctgaagc acgggccaat tccaccgtcc gcgaaaaaat
    22141 cttcgttgaa gggccaagct ttgtcgattt aaggaaacgc cgttcgacga tattgcggca
    22201 gtcattcgcg agggatgaat catgacaaac acgaaaaagc cgaaagcgcc gaaagcgccg
    22261 aaagcgccga aagcgcgaac caagaaaccc gctcacagga ccacgcctga gggcgtggtc
    22321 gttcttgtcg ccacccgtaa aggagcttgg ttttaccgcg gcgacaaagg gcggaagtcc
    22381 tggcaggccg atggacccca tttcctcggc cagatcatca atcatctggt gcttgatccg
    22441 cgcgaccgca agacggtgct ggccgcggcc agcaccgggc acctgggtcc caccattttc
    22501 cgctccaccg atctcgggcg cagctggcag gaagccaaac gcccacctgc ttttgcgaag
    22561 gcgcccgacg gtgaaaaagg aaggagcgtc aagcacacat tctggctcgc gcccgcccat
    22621 gcgaacgagc ccaacatttg gtacgccggg acctcgcccc aaggtctttt ccgctccgag
    22681 gacggcggca acaattggga gccattcggc acggtgaacg atgatccgca ataccgcgcg
    22741 tggatgggtg cggagcagga cggcacaccg gacgggccca agatgcactc gattctggtg
    22801 gacccgcgcg atcccgcgca tctctacatg gccatgtcgg gtggcggcgt tcatgaatcg
    22861 cataatggcg gacgaacctt cgcgccgctc gtcaaaggcc tcgaagtggt ggaaggattc
    22921 gatgcggcca acatcacctt ccatgatccc cattgcgttc ggctatgtcc gagcgatccg
    22981 gatcggctgt atcagcagaa tcattgcggc atctaccggc tcgaccggcc gtcggacgaa
    23041 tgggtccgcg tgggcagaaa gatgcccaaa cgggtcggcg atatcggctt tccgatggtg
    23101 gtgcatccgc gtgacgccaa cactgtctgg gtctttccga tggatggccg gaatgtttgg
    23161 ccgcgcacaa gtccagacgg aacgccgtcc gtgtatgcca gccgcaatgg cggcaagacg
    23221 tggcagcggc tcgattccgg tctgccgcaa agtcaggcgt attggacggt gaagcgccaa
    23281 gccatgacgg cggattcggc cgatcccgtg gggctttatt ttggaacgac cagcggcgat
    23341 ctttggatga gccgcgatga gggcgagaaa tggacgaccc tcgcgcggca tctcccggag
    23401 atctatgcgg tggaggtagc tgaggtgtga aagttctgat cccaacgccg ctgcgctcct
    23461 acacgggcac gcgcgaggtc gaggccgacg gtggaaccat cggcgcattg cttgcggaac
    23521 tcgaccgccg ctacccgggt ttgcgctttc gcatgatcga cgagctggac cgcgtgcgcc
    23581 cccatatgcg tgtcttcgtc aatggcgagc agaccttcga tctgacgcgc gcgcttgtcc
    23641 cggcggactc ggtgcagatc gtccaagctc tcagcggcgg gtgaagcttt cgtcgttggc
    23701 gagccgcgtt tcggcgtggg cgaggtcata gggcgtattg atgttgaaaa acggatcgat
    23761 cggatcggat gcaaagccca cgcgcgcgtt gagaaagagc gcgacgaaat catccatctt
    23821 gcgcaatccc cgatccacca gagcgccttg cgacgtagcg gtgatggtgg aactccacac
    23881 cgcgaacacc ggatgatggc gcgcaccgct cgcggcgata gcgacgggca cattctcgtg
    23941 ctgtgctcca tcgatcaggc ggtccaccag atcgatggga aagaatgggc agtcgcagga
    24001 aaaactcgcc aaccagcgcg cttgggagcg cttcgcgcgc atccattcga gcgccgccag
    24061 gataccggcc acgggcccga gaaatccccc gacggcatcg gggatcacgg gaaggccgaa
    24121 cccagcaaag cgttcggaat cgccattggc gttgatgagc aattcgccga cctgcggctt
    24181 tgcgcgtgca atcgtccgtg caatcagcgt ttcgccgccg agcgtcagga atgccttgtc
    24241 cacaccgccc atgcgctgcg accggccgcc cgccagaatc acaccaacga tgtcgttcgt
    24301 atcgcgcgtt gctagcgtcc caagtgaaag gggaaggaaa tcctttctgg atttccttcc
    24361 cctggcaatg cttgcgtttg acgcttttag aacaaaccga cgatcttgcc gtcagcattg
    24421 acgtcgatgg aatccgccga tggagtcttg ggcagacccg gcatggtcat gatttcgccg
    24481 cagatcgcca ccacgaattc ggcgcccgcg gagagccgta cttcgcgcac attgatggta
    24541 tggccttcgg gtgcgccctt ggagtcggga ttggtcgaga agctatattg cgttttcgcc
    24601 acgcagatcg gcgctttgcc gtagcccata gcctcgaagt tggcgagttg gtcccggacc
    24661 gatttgtcgg cggagatgtc cttggcgcgg tagatttcgc gcgcgatggt gcggattttc
    24721 tcgagcagcg gcatgtcgtc cggatagagg aatttcagtt tgctcttgcc gctatcgcaa
    24781 atcttcacaa cggcgcgcgc gacatcggca gccccttcac cgcccatcgc ccaatggtcg
    24841 gccatgtagc attcaacgcc caactgcgcg caggcggttt tgaccagctc gatctcggct
    24901 gccgaatcgg cgctgaaccg attgatcgag atcaccggct ccagaccgaa tttgcgcacg
    24961 ttctcaacat ggcgcttcaa attggacatg ccgctctcga gcgcggtcaa atcctccttc
    25021 ttcaaatctt ccttcttcac gccgccatgc atcttcaggg cgcggatggt cgcgacgagg
    25081 acgacgcaag acggttcgat gcctgccttg cggcatttga tgtcgaggaa tttttcgccg
    25141 ccgagatccg cgccgaaccc tgcctccgtc accacataat cggcgagctt caaagccgtc
    25201 gtcgtcgcca gcaccgaatt gcaaccatgt gcgatgttcg cgaacggacc gccatggatg
    25261 aaggccggcg tgccttccag cgtctgcacc agattgggcg aaagggcatc cttgagcagc
    25321 gccgtcatgg cgccttgcgc cttcagatcg ccggcgcgca cgggtttgcg gtcgcgcgta
    25381 tagccgacaa tgatgttgga aagccgtttc ttcagatcgt caagatcgga ggcgagacag
    25441 aaaatcgcca tgacttccga ggctacggtg atgtcgaacc cgtcttcgcg cggaaaacca
    25501 ttggcgacgc cgccgagcga attgatgatc tggcgcagcg cgcggtcgtt catgtcgagc
    25561 acacggcgcc aggcaatgcg gcgttgatcg atattgagcg aattgcccca atagatatga
    25621 ttatccagca aagccgcaag caaattattc gccgaagtga tggcatgaaa atcgccggtg
    25681 aaatgcagat tgatatcttc catcggcacg acctgggcgt agccgccgcc ggcagcgcca
    25741 cccttcatgc cgaaggatgg gccgagtgac ggttcgcgca ggcacaacat ggcctttttg
    25801 ccgatgtggt tcagcgcgtc ggtcagaccc accgtagtcg tggttttgcc ttcgcccgcg
    25861 ggcgtcggcg tgattgccga taccaggatc agcttcccat tgggtttatt cttcagcgat
    25921 ttgatgtaat ccatcgacac cttggctttg taatggccat agggatcgag gttctgcggg
    25981 tcgatgccga gccgtttttt cgcgatctcc atgatcggtg tcattttcgc ggcttgcgaa
    26041 atctcgatgt cggattttgg gttctgatgt tgagaagcgg gcatgaaaaa atccttcgat
    26101 ttgaaatggt tcagccgagg cgcgtgcctt gggcgagatt ggtggtcttg gcgaaatcgg
    26161 cggcagaggc cttgccgcca tcggcgggtt tcacccgcaa cactttgatg cggccgtcgg
    26221 cacagacgac ggagaaaccg tcggcggtga tttccaccac ttcgccgagc ttgccgccga
    26281 tatccttggg tgtcttggcg ggctgcggct tggcctcgaa aatctgcacc gtcttgccgt
    26341 cgatcatggt ccaggcgcca ggtgccgggc tgcagccacg aatcacttga tcgatctgtt
    26401 tgcagcattt gccccagtcg attttcgcat tgtcagcgcg gcaaagtcct tcataggttg
    26461 caaggttctc gtcctgtttg atgcgtgggg ccttgcccgc tttcacgagg tcgatggact
    26521 ccatcatcgc ttcgacgccc atcgggaaca gccggtcgaa atagacggaa cccagcgaat
    26581 ccgtgtcgct gatcgtcgtc tttttctgca gcaggacgtc gccggtatcc aggccattat
    26641 ccggccagaa gatcgacaat ccggtttcgg tctcgccatt gatgatcggc cagttgatgg
    26701 ccgaggcgcc gcgtcccttg ggcagcagcg aagggtgata ttgaatcgag ccataggtcg
    26761 gaatgttcag gaattcctcc ggcacaaaaa gggtgacgaa cgccatgacc tgaaggtcgg
    26821 gcgccagcag cttgaattct tcccacacct cttctttgcg ataggacgcc ggctgtagga
    26881 cggggatgcc cgccgcgacc gccgcgtctt tcaacggatc ggctttggcg ccgggtttct
    26941 ccggcgccac ataaacggca acaacgttct caccgcgcgc aataagcgcg tcaagaaccg
    27001 ctttgccaaa ggcctgctgg ccatgaacga cgatacgcat atttttcttc ccctcgattc
    27061 ctaatccctt cccaatttgt cccgcctcaa cgcgaggggg gcaggggtag ggctatgccg
    27121 cttcggcgcg tgacttttcc ggtggcgtga tcgcgccgga cgatttgatc tcggcgagat
    27181 cggattccgt atagccgagc atcttggtca ggatctccac cgtgtgctcg cccagaagcg
    27241 gagaacgctc cacctcgcaa ggcgaatccg agagcttgat cggatttccg acggtcaaat
    27301 atttgccccg gaccggatga tcgacttcca ccaccgtacc cgtcgcacga agcgattgct
    27361 cctcggcgat ttccttcatg gacaggatcg gacccaccgg aatgtcatgc tcgttgcaga
    27421 tatccatcac ctcgaacttc gtcttggtca tggtccattc ttcgatgatg ccgaagatcg
    27481 ctttcaggcg cggcaggcgt gccggcggcg tggcataatc cggatcggtc ttccattcgg
    27541 gcttgccgat cagatcgcag atttcactcc ataccggcgc ctgggtgatg aaataaatat
    27601 aggcgtcggg gtcggtctcc caacctttgc atttgaggag ccaggccggc tggccaccgc
    27661 ccgaatcatt gccggcgcgt ggaaccgcgt tgccgaacgg gatgccttcg ccatattggc
    27721 tgtattcggt gagcggcccg tgcttcaggc gctgctgatc gcgcagtttc acgcgtgcga
    27781 gattcagcac gccgtcctgc atggcgcaca atacccgctg tccgcgcccg gtgcggttcc
    27841 tctgatagag cgccgaaacg ataccgagcg cgagatgcag tccggttccc gaatctccga
    27901 tctgcgcgcc ggtgacgagc ggcgggccct cgcggaagcc cgtggtcgat gccgcgccgc
    27961 cggcgcattg cgcgatgttc tcgtagacct tgcactcctc ataagggccg gggccgaagc
    28021 ccttgaccga ggccatgatg atgcggggat taagctgatg cacgcgctcc caggtcagcc
    28081 ccatgcggtc gagcgcgccc ggcgcgaaat tctccaccag cacgtcgcag gtcttcacca
    28141 gacgttcgag gatctctatg cccttgggat ttttcgaatc gatggtgatc gaacgcttat
    28201 tggcgttgag catcgtgaaa taaagcgaat ccgcgccttt cacgtcacgc aattgggtgc
    28261 gcgtgatgtc gccgacgccg gggcgttcga tcttgatgca gtccgcgccc atataagcga
    28321 gaagctgggt gcaggtcggt cccgattgca catgggtgaa atcgagaatg cgcacgccgt
    28381 cgagggcctt gccatcctgg ccccagggtt ttgtggacgg agccggatag ccgttgccat
    28441 ttgccatggt ggctttgcgt ttcggcgccg gcttcttggc ggccgctttg cgggcgacgc
    28501 gcagtttgtt gacgaccagc tttttcggcg cctgcttctt tgctgctttc gctgtcactt
    28561 tcttcgcaga ttttttggga gccggtttct tcaccgccgc cttggccttt ttggcttttg
    28621 ctttcgccat gtctgggttc ctccaatcga atgtgcttgt gggagaatta tttcttcttc
    28681 aacgcgcttt gcgggttcag attgccgatt cggccgctct cgctgccggc ggccgggtcg
    28741 agcaccgcgt tgatgagcgt cggtttgccg gaatccatcg ccgcgtttac ggcgcgcttc
    28801 agctcgtccg gtgaggtcgc attgacgcca acgccaccga aggcctcgat catcttgtcg
    28861 taacgcgaac ctttgacgaa cattgtcggc gcggggtccg aactgacttt gttgacgtcg
    28921 gtaccgcggt aaatgccgtc attgttgaag atgacaatac agaccggcaa attgtaacgg
    28981 cagatcgtct cgacctccat gccagagaaa ccgaacgccg agtcaccttc aattgccagc
    29041 acgggtttgc cggtttcgat cgccgcggcg atcgaatagc ccatgccgat gcccatgatg
    29101 ccccaggtgc cgacgtcgat acgcttgcgc ggctgataaa cgtcgatgat gccacgcgcg
    29161 agatcgagcg tgttggcccc ttcgttgacg aggatggcgt ccggccgctc cttgacgatg
    29221 gtccgtagca cgccgagcgc gccgtgatag tccatcggca cgttgttgtt catcaggcgc
    29281 ggggccatct tggcgacgtt ctcgtcgcgt ttcgtggtca ccgccttcat ccagtcggct
    29341 gacgctgcgg gccacgaacc catcgccgtg agcatcgcgt tgacgcagga gccgatatcg
    29401 ccgaccacag gcgcgacgat ctcgacgttg gagtccatct ccttcggctc aatgtcgatc
    29461 tggatgaact tcttcggttc ggtgccccag gtcttgccct tgccgtgcga cagcagccag
    29521 ttgaggcggg cgccgatcag catgacgacg tcggattgcg ccagcacggt cgagcgcgca
    29581 gcacctgcgc attgcggatg cgtgtcgggc agcaggccct tgcccatgct catcggcag
//
LOCUS       DASYEG010000184         4434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4553325, whole genome shotgun sequence.
ACCESSION   DASYEG010000184 DASYEG010000000
VERSION     DASYEG010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4553325"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VGM72_08350"
     CDS             <1..439
                     /locus_tag="VGM72_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4265314.1"
                     /translation="DEIESAARSAAAHEFIAALPMGYDTRTGEGGLRLSGGQRQRIAI
                     ARAMLRDAPILLLDEATSALDAENERLVQDALRRLMKGRTTILIAHRLSTVLDADAIY
                     VLDRGKVAEHGTHGELMARGGLYAKLYKHETQENEAAPATHLG"
     gene            462..1724
                     /locus_tag="VGM72_08355"
     CDS             462..1724
                     /locus_tag="VGM72_08355"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEY4265315.1"
                     /translation="MALALYRGVMGGLSPAVLRARVKHGKEDGARLAERKGIASRARP
                     DGTLIWIHGASVGESLSVLPLVSALLERPNRHVLVTTGTVTSAKLMEERLPMGAFHQY
                     VPIDSLQSVRRFLAHWQPDGALFVESELWPNLILETRRRSIPMALVNARLSAQSFRGW
                     HYAPRLARRILSCFDACLAQDDAIAKRLIALGAGDVRISGSLKADAPPLPVDEKALDD
                     FRASTGTRPIFLAASIHPGEVDAILQAARNLLGPHPGALTVIVPRHPQRGADIAAEAA
                     LRGFSVRRRAAGMLPSPETQIYIADTLGELGLFYRATPFAFIGGSLVPHGGQNPLEPA
                     RLETAILAGPQTQNFEEIFRVLLHAQGEGRVHSPEELGLLAARLIADPSVAARLGANA
                     RAAAETLGGALAATITLTESMLRSHARA"
     gene            1711..2688
                     /gene="lpxK"
                     /locus_tag="VGM72_08360"
     CDS             1711..2688
                     /gene="lpxK"
                     /locus_tag="VGM72_08360"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEY4265316.1"
                     /translation="MRAPEFWKTNGVAATLLSPLGVLYGWSVRARKARSHPFRPKARV
                     VCVGNLTAGGSGKTPTAIALAHALAARGRKIVFLSRGYGGSLRGPVLIDHAHHSPAQT
                     GDEPLLLAVHGATIVARDREQGARFSERLDADIIVMDDGHQNFQIAKDVSLIVVDAQA
                     GFGNGKLIPAGPLREPVAPGLARADGVVLMGDGRPPLGSFAGPILRARLVPTAPDALK
                     GRSVFAFAGIGRPEKFFAMLRALGANVLAAQPFPDHHRFTVFELSALKESAQKTGALL
                     VTTEKDYVRLDPMARHDIVPVPVHAAFNDISALGALLDRIIGAGNLGRP"
     gene            2685..3668
                     /locus_tag="VGM72_08365"
     CDS             2685..3668
                     /locus_tag="VGM72_08365"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY4265317.1"
                     /translation="MSEATAPKTALPDASARSFGRNLRYLLEAAGFFLIVGFFRVFDI
                     DRASAIGGWIGRNLVGRTYLSRRPLRNLRTAFPDKSETEIRAILNAMWDNLGRVMGEY
                     PHLDRLHWAGAAPRITMSGEEYLNEAKAHSKGVMLVSGHLANWEIMPFVGREYGFTGG
                     TVVRPPNNPYVSRWMERVRARNGMQEQIPKGPQGVRRTFSLLRRGDAICLLVDQRTSE
                     GIKVPFFGRDAFTTPAPAALALKLGAVIVPVSNVRTRGAHFHMRAYPIIEPPNTGDPD
                     RDLVELTSRITRFIEDRVRENPGQWLWIHKRWVKDDAPLGKRAHALSQDGR"
     gene            complement(3672..3896)
                     /locus_tag="VGM72_08370"
     CDS             complement(3672..3896)
                     /locus_tag="VGM72_08370"
                     /inference="COORDINATES: protein motif:HMM:NF021392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2093 domain-containing protein"
                     /protein_id="HEY4265318.1"
                     /translation="MNQFERDIGGRPALLQYLDGEFRVVTPGNFVVCAVTGTKIPLEA
                     LRYWSVDLQEAYAGPSAALKRAQEKGFTPR"
     gene            complement(3942..>4434)
                     /locus_tag="VGM72_08375"
     CDS             complement(3942..>4434)
                     /locus_tag="VGM72_08375"
                     /inference="COORDINATES: protein motif:HMM:NF014640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY4265319.1"
                     /translation="PRQRFDSSAGRLSAALFQNLQRHRGQFARVSALLRPRIITAEIE
                     RGRDCVADDHARLIRGFRQGLRAAGGSLDSCARLLDSLSYRAILARGFALVRGSDGHL
                     KRRASMITPGEGLTLIFADGEAKTGASLKTEAKAEARPSRNPKGPELPKGPRRAKGQG
                     DLF"
BASE COUNT          759 a         1451 c         1416 g          808 t
ORIGIN      
        1 agatgagatc gaatccgcag cccgttccgc tgccgcgcat gaattcatcg ctgcccttcc
       61 catgggttat gacacgcgca ccggcgaggg gggcttgcgg ctttccggcg gtcagcgtca
      121 gcgtatcgcg atcgcgcgcg ccatgctgcg cgacgcgccg atccttttgc tcgatgaagc
      181 gacgtccgca ctggatgccg aaaacgagcg ccttgttcag gacgcgctgc gccgcctgat
      241 gaaagggcgt accaccattc tcatcgccca ccgcctcagc accgtgctcg atgccgacgc
      301 gatctatgtg ctggaccgtg gcaaagtcgc cgaacacggc acgcatggcg aattgatggc
      361 gcgcggcgga ctctatgcca agctctacaa acacgagacc caggagaacg aggctgcgcc
      421 cgcaacacat ctcggttgag ggcgctgggc gaggcggcgg ggtggcgctc gcgctctatc
      481 gcggcgtcat gggcgggctt tcgcccgccg tgctgcgtgc ccgcgtcaaa cacggcaagg
      541 aagatggcgc gcggcttgcc gaacgaaagg gcatcgcatc gcgcgcgcgc cccgacggca
      601 ctctcatctg gattcatggc gcgagcgttg gagaatcgct ctccgtgctg cctttggtca
      661 gcgcacttct ggaaaggccg aaccggcatg tgctggtcac caccggcacg gtgacctccg
      721 cgaaattgat ggaggaacgc ctgccgatgg gtgcgttcca tcaatatgtg ccgatcgatt
      781 cgctccaaag cgtgcgccgt tttctcgccc attggcagcc tgatggcgcg ctgttcgtgg
      841 aatccgaatt gtggcccaat ctgattctag agacgcgacg gcgctccatc cccatggcgc
      901 tggtcaatgc gcggctgtcg gcacaatcct ttcgcggctg gcactacgcc ccgcgtttgg
      961 ctcgccgcat cctgtcgtgc ttcgatgcct gtctggcgca ggacgacgca atcgcgaagc
     1021 gactcatcgc gctcggcgcg ggcgatgtgc gcatatccgg aagcctcaaa gccgatgccc
     1081 cgcctttgcc ggtggatgaa aaagcgcttg acgattttcg cgcaagcacg gggacgcgcc
     1141 cgatcttcct ggcggcaagt atccatcccg gcgaggtgga cgcgattttg caggccgcgc
     1201 gcaacctgct cgggccacat ccgggcgcgt tgacggtgat cgtgccgcgg cacccccagc
     1261 gtggagcgga catcgcggcc gaggcggctc tgcgcggatt ttcggtgcgg cgccgcgcag
     1321 ccggtatgct tccctcgcca gagacgcaga tttacatcgc ggatacgctc ggggagcttg
     1381 gcctgttcta tcgcgccacg ccgtttgcgt tcatcggcgg aagcctggtg ccgcatggcg
     1441 gccagaaccc gctggaaccc gcaaggctgg agactgccat tctcgcgggg ccacagactc
     1501 aaaacttcga ggaaatcttc cgcgtgctgt tgcatgcgca aggcgaagga cgcgtgcatt
     1561 cgccggagga attaggcttg ctcgccgcca ggctgatcgc cgatcccagc gtagcggcgc
     1621 ggctgggcgc caacgccaga gccgcagccg aaacattggg cggcgcgctc gcagccacca
     1681 tcacgcttac cgaatccatg ctccgatccc atgcgcgcgc ctgagttctg gaaaacgaac
     1741 ggcgttgcgg caacgttgct gtcaccattg ggcgtgcttt acggctggtc ggtgcgggcg
     1801 cgcaaagccc ggagccaccc gttccggccc aaggcgcgcg tcgtttgcgt cggcaatctg
     1861 acggccgggg gatcgggcaa gacacccacg gccatcgcgc ttgcacacgc tctcgcagcg
     1921 cgcgggcgaa agatcgtctt cctttcgcgc ggatatggcg gaagcttgcg cggcccggtg
     1981 ctgatcgatc acgcccatca ttccccggcg caaaccggcg atgaaccttt gctgcttgcg
     2041 gtccatggcg ccaccatcgt cgcgcgcgac cgtgagcagg gtgcgcgttt ttccgagcgc
     2101 ctggacgccg acatcatcgt gatggatgac ggtcatcaga atttccagat cgcgaaggat
     2161 gtgtcgctca ttgtggtgga tgcgcaagcc ggtttcggca acggcaagct gattccggcc
     2221 ggccctctgc gcgaaccggt cgcgcccggt ctcgctcgcg ccgatggcgt cgtgctgatg
     2281 ggcgatggca ggcccccact tggctccttc gccggaccaa tcttgcgcgc gcgcctggtg
     2341 cccaccgcgc cggacgctct taagggacgc agcgtctttg ccttcgcggg gatcggccgt
     2401 ccggagaaat tctttgcgat gctgcgcgcg cttggtgcga atgtcctggc ggcgcagcct
     2461 tttcccgacc atcatcgctt caccgttttc gaactctccg cgcttaaaga atcggcgcaa
     2521 aaaactggcg cgctcctcgt caccaccgaa aaggattacg tccggctgga tccgatggcg
     2581 cggcacgata tcgtgccggt ccccgttcat gcggcgttca acgacattag cgcattggga
     2641 gcgctgcttg acagaattat cggcgcgggg aatctcggca ggccatgagt gaagccaccg
     2701 cacccaagac cgcccttccg gatgcgtccg cgcgctcctt tgggcgcaac ctgcgctatt
     2761 tgctggaagc agccggattc ttcctgatcg tcggtttctt ccgcgttttc gatatcgacc
     2821 gggcctcggc catcggcggc tggatcgggc gcaatctcgt ggggcgcacc tatctgtcgc
     2881 gacgcccgtt gcgcaatttg cgcaccgctt ttcccgacaa aagcgagacg gaaatccgcg
     2941 cgatcctgaa cgccatgtgg gacaatctcg gccgcgtcat gggagaatat ccccatctcg
     3001 acaggctgca ttgggcgggc gcggctccgc gcatcaccat gagcggcgaa gaatatctca
     3061 acgaagccaa ggcccacagc aaaggcgtga tgcttgtctc cggccatctc gccaattggg
     3121 agatcatgcc gttcgtcggg cgcgaatatg gcttcaccgg cggcaccgtc gtgcggcctc
     3181 cgaacaaccc ctatgtcagc cgctggatgg aaagggtgcg ggcccgcaac ggcatgcagg
     3241 agcaaattcc caagggtccg caaggcgtgc ggcggacctt ctcgctattg cgccggggcg
     3301 atgccatctg cttgctggtc gatcagcgca cgagcgaggg tatcaaggtg ccgttcttcg
     3361 ggcgcgacgc gttcacgacg cctgcacccg ccgcgctcgc gctcaagctc ggcgcggtca
     3421 tcgttccggt gtcgaatgtg cgcacgcggg gggcgcactt ccatatgcgc gcctatccga
     3481 tcatcgagcc gccaaatacg ggcgatcccg accgcgatct ggtggagctg acatcccgga
     3541 tcacacgctt catcgaggat cgcgtacggg aaaatcccgg ccaatggctg tggattcaca
     3601 agcgttgggt gaaggacgat gcaccgctcg gcaaacgcgc gcacgccctc tcccaggacg
     3661 gccggtaaac tctatcgcgg agtgaagccc ttttcctgcg cccgcttgag ggccgccgaa
     3721 gggcccgcat aggcctcctg gaggtcgacg ctccaatagc gcagggcttc gagcggtatc
     3781 ttggtgccgg tgacggcgca gacgacaaaa ttgccaggcg tcaccacccg gaattcaccg
     3841 tccagatatt gcagaagggc ggggcggccg ccgatatcgc gttcgaactg gttcatggtt
     3901 ttcagactgt agcgcgccgg gcggctaaaa accaacgcct atcagaacag atcgccctgc
     3961 ccctttgcgc gcctaggccc tttcggaagc tctggccctt tggggttgcg cgacggccgc
     4021 gcttccgctt tggcttccgt tttaagggac gcccccgttt tggcttcccc atcggcgaaa
     4081 atgagcgtca gcccctcgcc gggcgtgatc atggaggccc gccgcttcaa gtgaccgtcg
     4141 gaaccgcgca cgagggcaaa accgcgtgcc aggatcgcgc gatagctgag gctgtcgagc
     4201 agccgggcac acgagtccag gctcccgccg gcggcacgca gcccttggcg gaagccccgg
     4261 atcagccgcg cgtgatcatc ggccacgcag tcgcggccgc gctcgatttc ggcagttatg
     4321 atgcgcggac gcagcagcgc ggagacccgc gcgaattgcc cccggtgacg ttgcagattc
     4381 tggaacagcg cggcgctgag gcgccccgcg ctcgaatcga aacgctgacg cggc
//
LOCUS       DASYEG010000185        21117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_204452, whole genome shotgun sequence.
ACCESSION   DASYEG010000185 DASYEG010000000
VERSION     DASYEG010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 21117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21117
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204452"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..1018
                     /locus_tag="VGM72_08380"
     CDS             236..1018
                     /locus_tag="VGM72_08380"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY4265320.1"
                     /translation="MKIKANGISFNCQVDGREGAPWVVFSNSLMTNLSMWDEQAAALS
                     GDFRVLRYDQRGHGGSDAPPGAYSFDVLTADAVALFNAFGIAKAHFVGLSMGGMTAVA
                     LAGQHPHRVERLVPCDCGPASTPQSAQQWAERIALAQEKGMEALVEPTVGRWFAAEFL
                     NSDKAAVDKVRAMVRTTPLNGFAGCAQALSNFDLRPNLAGIKHPTLFLCGAKDAALGG
                     TKALNAGVAGSKLMEIPDAGHISNIENPTMFTRALREFLSAP"
     gene            complement(1084..1512)
                     /locus_tag="VGM72_08385"
     CDS             complement(1084..1512)
                     /locus_tag="VGM72_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265321.1"
                     /translation="MNKVTKFAIGAAMACGIAIAAAAPAEARGVNFGIGIGVPAGPAY
                     YGAPYYGYGYGPSCYDYYDAYDCGYAPYYGPAYAGPAFVGGYWGGYGYRGGYANHRGY
                     DRGRFVSHGGLGRGGFAGHARGGAGPRGGGSGPHGGGHHR"
     gene            complement(1715..2359)
                     /locus_tag="VGM72_08390"
     CDS             complement(1715..2359)
                     /locus_tag="VGM72_08390"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY4265322.1"
                     /translation="MSLKLYYHPLSSFCHKVLIALYENGVPFEPVLVDLSSEESSAAF
                     KKIWPIGKFPVLRDEAKGLTIPESSVIIEYVDTHYPKGARLVPQEAEAAWRTRLEDRF
                     YDLHVHMPMQKIITDRLRPSGQNDSFGVQEATQQILTAYRLIDAGIAGKQWAMGDDFS
                     MADCAAAPALFYGSMAVPFGAHKNLAAYLERLKARPSYARVLKEAEPYFKYIPR"
     gene            complement(2378..2812)
                     /locus_tag="VGM72_08395"
     CDS             complement(2378..2812)
                     /locus_tag="VGM72_08395"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4265323.1"
                     /translation="MARVSTYLNFPRSTEEAFLFYKKIFGSEFTMPIARFRDIPPNPG
                     HPLAEADKDLVMHVELPILGGHVLMGTDAPESMGFKLVQGNNMFINLEPDNRAETTRL
                     FNALSAGGKIEMPLAEMFWGGFFGSLTDRFGIHWMFNCASKN"
     gene            complement(2859..3332)
                     /locus_tag="VGM72_08400"
     CDS             complement(2859..3332)
                     /locus_tag="VGM72_08400"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY4265324.1"
                     /translation="MTVKNDDSTGTADREIGATRIYNVPRTLVWQAWTDPRHVAQWWG
                     PNGFTNSISEMNVKPGGVWRFIMHGPDGTDYPNKIQFLEVVKPERLVYVHGDESDPDQ
                     FHVTVTFAEQDGKTTLTMRSVFRTKAERDFVVEKFGATEGMNQTLGKLGEYLAKM"
     gene            complement(3316..3681)
                     /locus_tag="VGM72_08405"
     CDS             complement(3316..3681)
                     /locus_tag="VGM72_08405"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY4265325.1"
                     /translation="MAPDRLSTTFAALADPTRRAILARLALGETSVTELAEPFDMSLP
                     AVSKHLKVLERAGLIARSREAQWRPCRIEPAALKDVDDWLGDYRRMWDARLDRLGDYL
                     IKLQAAKPPVEGKKHDRKK"
     gene            3879..5081
                     /locus_tag="VGM72_08410"
     CDS             3879..5081
                     /locus_tag="VGM72_08410"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY4265326.1"
                     /translation="MSVVAKENPGRASAAERVEEMNTETAKLHAWIRTQANAPAQRPW
                     FTDMGETGGTLAKGRVAANQMKMLAHRWRWREIYPYLRKISEIATEAKGELLERTDRQ
                     GILLTNPGLGGRLQITNTIRCAVAIYNHGDIAPAHVHSPNASRTILSDKGGYTNVEGE
                     RCECRRGDIVFTPNGTWHDHGNDDPDPMIWIDMLDWPLMEYLDSVWVDQDYIAATQSN
                     TKSQATKYADGHSTRMYGHGGLKPVFASNQRGWGQKSTPNFHYKGVDVMDALTGLRGE
                     EGDPFEGIKMQFVNPVTGGPVFASLDYCAQLLRPGEELALKRETSSTFIVVMEGEGFS
                     EIGGQRFDWEKNDILAVPNFLWRRHVNTGKNDAVLYTVSDAALMRALGQYRAQGKTAD
                     GSVTQIER"
     gene            complement(5130..5978)
                     /locus_tag="VGM72_08415"
     CDS             complement(5130..5978)
                     /locus_tag="VGM72_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY4265327.1"
                     /translation="MKLASYIAGGKPAFGQVTEGGIVTLSDRMGERNIGLKDALAEDG
                     LLDELLHLAKGAEPDMELDGLKYLPTIANPGKILCVGINYRSHAAEHGHAVAEKPNIF
                     TKFTDCLLGHEGEMWRPKASTQLDFEGELVVVMGKGGRAIPAADALSYVAGYTCFCDG
                     SVRDFIKTSLITGKNFPMTSPLGPWMVTADEIPDPSKLTLTTRLNGQVMQHSGTDMLM
                     HDVPALIAYCSAFTALSPGDIIATGTPEGIGARRNPPVWMKAGDVLEVEISGIGTLRN
                     RIVDEP"
     gene            complement(5975..6832)
                     /locus_tag="VGM72_08420"
     CDS             complement(5975..6832)
                     /locus_tag="VGM72_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY4265328.1"
                     /translation="MPKSNLKNLINDLVIANRILANEDVVDAYGHVSLRHPDDPTRFF
                     LARSLAPEFITADDIMEFTLNGEPVKKGGPSPYLERFIHGGILEARPDVMAVVHAHAE
                     DVLPFGLTNGTPLKPVIHSGSFIGSTVPIWDIADKFGDTNLLVANVEQGQDLAKCLAN
                     NNVALMRGHGFAAASRSLIEVVRMSVYLPRNARALQKAKALGGYVKYLRQGEIDARNR
                     GYSPYSVETWRAWHYWATKAGVGDMVTRPDAGVAVSKKGAKLAKKPAKKAAAKKPAKK
                     TRTAKGRKK"
     gene            complement(6865..7863)
                     /locus_tag="VGM72_08425"
     CDS             complement(6865..7863)
                     /locus_tag="VGM72_08425"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4265329.1"
                     /translation="MGNLQLSFACALYDRMEPLYTGEVKPDGIDLNFIAIDEPRPIFD
                     RMSGGQEFDVAEYSSSEFVQRFANKQCPFVALPVFPSRCFRHGFIAINKKSGIKTPKD
                     LEGKRVGVPLYTMTAAIFIRGLLQHEYGVDCEKIHWVQGAVNHSGSHGDPHVLPLLKP
                     IAIEQAPKGRALNDLIMEGYVDATLGTSLPESVRTHPDIGRLFPDYVEQEKDYYKRTR
                     IYPIMHLVAIRRDIYERYPFVATSLYNAFERSKRIALKKMYNLRALRYMTPWLMRDID
                     EIHDVFGGDPWPYGVEKNRPTLEALVTYLADQHLIADAPKVDDLFVRNYGEVDSWF"
     gene            complement(7962..8837)
                     /locus_tag="VGM72_08430"
     CDS             complement(7962..8837)
                     /locus_tag="VGM72_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY4265330.1"
                     /translation="MSATRLTSFSIHGRTSFGAVTDAGMIDLGGRLGGRYPSLLSLIQ
                     AGEAGLKDARAACAFPFADYPLSEVQFLRPLLAPEKIVCVGINYPERAGEYKDGREKP
                     KYPNLFVRFPDSLVGHGQNLVRPKVSEKFDYEGEIVLVIGREGRQVPKEHALSMIYGL
                     TLGNEGTLRDWLRHGTLNVTQGKNFDASGSLGPWIVPADEVDPGKPLHLMTRINGELR
                     QDDNTERLTWGFAWLINYITQFATLKPGDLIFTGTPVGAGGHQTPPAWLKPGDVVEVE
                     VPEIGVLRNSVVDEI"
     gene            8968..10188
                     /locus_tag="VGM72_08435"
     CDS             8968..10188
                     /locus_tag="VGM72_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY4265331.1"
                     /translation="MHDVAAKVHWHEPTGGKRAGFGKWGRVKTPYDAFMESEGIPCFR
                     DIGIAKVQNLPLVPWKRLGGRGTYIQLHGTESKWGCYLVEVPGRGALNPEKHMFEKIF
                     FVVEGRGSTEVWQDGDTKRHTFEWQQGSLFSIPMNAYHRIVNAASAPALLLAGTTAPV
                     VLNTLQSVDAVFNSPIQFRDRFSGADDYYKYNDDVEPDPVRGLAMRRTNLVPDAVNCD
                     LPLDNRRSPGFRRVEPFMTGNSFYFWIGQHENGRYSKAHAHTSAAVLICIKGKGYTHT
                     WPERLGTTPWKDGHQDQVKRIDYEPVGMISAAPGGARWYHQHFGVSKEPLRLLAWFGP
                     HNPGREPGPPGEQHIDYTAMDIPEGGTSIPYWMEDPKVKADWEAKIAENGVESRMLPE
                     FFDKNFKGALPKGV"
     gene            10337..11446
                     /locus_tag="VGM72_08440"
     CDS             10337..11446
                     /locus_tag="VGM72_08440"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265332.1"
                     /translation="MTLIINNDDVAKVLTMEDTIATLEKSYLDIASKEAVCRPRIDIR
                     IPTSDPAKNYQWGTMEGGSTAGYFAIRMKSDIAYETVYNGVRTQEKYCSRPGLFCGLV
                     LLTSIETGEPLAFINDGVLQHMRVGGDGGIGVKYMANADAATVGMLGSGGMAHSNMEA
                     FMTVRPIRRLQIFSPTKENRERFGREMAEEHGIDVKVCDRPEEIYEGADIVAACTDSA
                     VPVLDGTRVEKGAHVINIGGSGVPDDETLKRIDVYLRFGDAPPPSSRPGLAFDDEHIG
                     WEARPGQPKHGDGRGVRKRGHGVLLPEKRISLADLVQGKVKGRTARDQITYSERGNLQ
                     GAQFFAVAGVVYDIAKREGLGHKIPTEWFLQTIRN"
     gene            complement(11451..11963)
                     /locus_tag="VGM72_08445"
     CDS             complement(11451..11963)
                     /locus_tag="VGM72_08445"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEY4265333.1"
                     /translation="MSQGPDVVIVFGAAVRADGSPSGALRRRVEAALNYGRDRSVTFL
                     VTGGLGIYPPVESVVMANMLRAEGVPEDAIVQENQSKTTLESVIRCTAIIRGLPDITR
                     VMVCSDAYHVARCRWLLRLSGIRAGGIGAASGRAANPWQKWLWFQLREYPAFVKDTIL
                     ILVRRATNCL"
     gene            complement(11963..13624)
                     /locus_tag="VGM72_08450"
     CDS             complement(11963..13624)
                     /locus_tag="VGM72_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265334.1"
                     /translation="MVKYLLLGLGGVAVIVFGVYYFQSRTTPTVMGADGRVVQVAALM
                     NAAENTCLSGKKLSGTASIDAAAAVDKLSGKGSATTQVEDLKGAVDVVNESLKLQEND
                     DIRQCMQTYTNQIFALITGKAVDDSPARQLDIRFSLTFMDPKNPVYDSAAIFIGEKFR
                     SAQFKYLVPDPRLAVPPDSVYDWPNVLMPSKQHPYSAVLHRVVRDGYRVESSKGTEIC
                     LVDGKSPPGGADLYAVLDCNESSGQCKLKDRDDPGWVSFCAPEKSTSLSWPGLISTAY
                     AEETPTKVWNVPSLETLRGRKDLRGAGFTEFHIEAPSLPGEKIDSVVLDLKVNGTPVR
                     IDGVDPEYRVLPYTSARSLDVEFALQNLDFAGINSGCDVIEAKLQPVSDGRISGAPIA
                     LKRSYVALRNAEPVKVQTAGREFTWTGIYIRPDNAYDYEVLVNSAQLSDLQSKPQLDN
                     ARLRVNTAKQQVDRMNLTFRGMRVVGVVRPPLTTPSFGTALGVIAPTGQIQFTFDQAT
                     AKEFKASVVAEAQRLPNGSKVVRSDTFVYPLNKNVDAPAGICLPS"
     gene            complement(13698..14174)
                     /locus_tag="VGM72_08455"
     CDS             complement(13698..14174)
                     /locus_tag="VGM72_08455"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY4265335.1"
                     /translation="MSWNIRRVVTGHDDDGKAIVKIDEICSNVRSGRERHHSCVIWST
                     GSFPADNSGDEDGALRPVGSTDPGGAVFRIVRYDPGVAPRSHRTDSIDYAVVISGEID
                     MEMDGTFVHLNQGDCLVQRGTVHNWINRGTQPCVIAFALIAAEPVKRKGDVLHMMG"
     gene            14256..14525
                     /locus_tag="VGM72_08460"
     CDS             14256..14525
                     /locus_tag="VGM72_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265336.1"
                     /translation="MDSEDKNDGVKGKILQWPLARGPLRVRRQPALALPAIAAGALAI
                     GALAIGALAIGSLAIGRLAIGRTRVQKMEIGDLTVGRLRVLERDD"
     gene            14572..15609
                     /locus_tag="VGM72_08465"
     CDS             14572..15609
                     /locus_tag="VGM72_08465"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY4265337.1"
                     /translation="MERRIFLAGAAGAMAAAALPEMAKADELPLGPLPGTRYPESRIQ
                     ALDPRFKYRIGNAYIERVATNCRWCEGPVYFRDGGYLLWSDIANNRIMRWSEDDGHVS
                     VFRSPSNFANGHTRDREGRLISCEHDTRRVTRTEYDGSITVLMDKYNGKPLNAPNDVV
                     VASDGAIWFTDPGFGKLSNYESTGKAELELPPNVYRLDPRSGRATVVYVPDAGRPNGI
                     AFSPDEKKLYIIDSAIPAPASSDIRVFDVNGDKIVNGKIFAADFTKGSTDGMRLDVDG
                     NVWCSMGNGDPAEDGVRCYAPNGDLIGKIHLPETCANLTFGGKKKNRLFVCGSTSIYS
                     VYIDTVGAMVP"
     gene            complement(15635..16555)
                     /locus_tag="VGM72_08470"
     CDS             complement(15635..16555)
                     /locus_tag="VGM72_08470"
                     /inference="COORDINATES: protein motif:HMM:NF018420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HEY4265338.1"
                     /translation="MTNSASHRQSRSPMRAMLLTVSALALFGLSSSAPAASGSEQVDL
                     KLVLATDVSGSINEEEFDLQRQGTAAAFADPEVVKAIQSGALGRIAVAMFDFSSPEFA
                     KMVIDWHIVKDKASAQAFSELVSGAPRSPGRRTSVSSALQLGYTMIEATEKDIVATRR
                     VIDVSGDGPNNDGENMREVHDRVIGTGIVVNGLPIMDDNANGYFPGLDKYYAACVAGG
                     RASFVVVAKSFRDFGLAMRHKLILEISGIDPMTKFADRMPGENGLLRQVAASPEGAPV
                     GAPLPVIRPGQNEFSDHCDIAGGFGGFNRF"
     gene            complement(16666..17313)
                     /locus_tag="VGM72_08475"
     CDS             complement(16666..17313)
                     /locus_tag="VGM72_08475"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEY4265339.1"
                     /translation="MATKIVMGAIAGAFGLTAILASPAGADIIADWNTAKAPPPPELK
                     EVTVDPSTTALLFLDIMKGGCSARPRCIAAVPNMKRLHEEARSHNMVVWYSLVGSNGK
                     ATPDDIMDAAIKPRAGEWYRQGGPDKFLGSTLQATLRQAGIKTVIVCGNSFQGATVGT
                     ASGAAQRGYKVIVPVDCSAGEDAYHEQYAAFHLAKGGPVAVTSQVTLTRSTMIKF"
     gene            complement(17536..17937)
                     /locus_tag="VGM72_08480"
     CDS             complement(17536..17937)
                     /locus_tag="VGM72_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4265340.1"
                     /translation="MSVTRLLMIDDEPYIGALVRRVAEGLDYEMRFVTLADEFKRTHR
                     SLDPDVVILDLSMPDIDGIELLKFLATEKSRARILILSGFDARILNGARRLGEDRGLH
                     MAGIVSKPVRIAELRTILETLKTTQPDRPND"
     gene            complement(17934..20315)
                     /locus_tag="VGM72_08485"
     CDS             complement(17934..20315)
                     /locus_tag="VGM72_08485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4265341.1"
                     /translation="MVAPQRSLGGILLLITGTLTLIIVLLAAKDMYGNWRRLADIRAL
                     REATISSDQLFDAIEKLSVERDIALSMMQALDTDTIDDLRPRLNESRQAADESLRESM
                     TTLNQFSFPEVAKLRAEMESHLAVIQKLRPEIDGAVDLPKEQRNPDLSKRWSAEVTAS
                     MQDTENLWIDFVRYFTNIDPVVTQHLWFKQLLRTITDYTGRERSLVGQLIAENATPTP
                     AQVAQLLRGQGIVEASWSMSRSLAGQSGLYASIAPAYTDASSHYSTIHDMIQGMIYAP
                     DAGQGAEYPISADLWFELSNQASDSLGTLKNRSIAQTRSYVEGLIAQTVEGITLQVTV
                     FLLALLLCAYSFWVIIGRVIRPINQMVVALLQATRGEIVAPVLPTGRQDEIGKLSIVL
                     HAFQQNVEEMKRTTKQLVQAQKMEGVGQLTGGIAHDFNNLLAIIVGNLDLLEEELPPN
                     SKAREMAHDALTASLRGADLTRQLLAFSRRQTLEPKVLCLNEVVSRTTELLRRTLGGR
                     IEIGMRLADDLSPALADQTQVESALANMAINARDAMPEGGHLTIETANKYLDEQYQME
                     NPEVVPGNYVMLAVSDTGSGIPPEILDRVFEPFFTTKAEGKGSGLGLSMIYGFAKQSR
                     GHVKIYSEIGHGTTIRLYLPRANAAMELPKEGVAELKTLVGTATVLVVEDNADVRSVA
                     VRQLGELGYHVLEAANADGALTILLEDRTIDLLFTDVIMPGAMTGDLLAREAKRLRPD
                     LKVLFASGFAEASVQNGSRSPDIDGHNLLSKPYRKPDLARRILETLQSRDGLS"
     gene            complement(20318..21019)
                     /locus_tag="VGM72_08490"
     CDS             complement(20318..21019)
                     /locus_tag="VGM72_08490"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HEY4265342.1"
                     /translation="MVILKMMGLARKIAVANRTATHALAALENAIKTACLIKSSSFAG
                     WLNAAPSVLRVRAIIAILLASFLALPAQAADVQLVEQQIKAGLLYNFLKYTQWPPESA
                     QREGTTKVCLFGGDPFDGHLQPMAGRTVNEHVIEVRSVRAVTDIDDCSLLFVRADQKA
                     NWPELQKALAGKSVLTVSDFEGFALSGGMIEFTRIANRISVTINTEPIGSAKLQVEDR
                     LLRLASMVRTTTPGP"
BASE COUNT         4319 a         6386 c         6212 g         4200 t
ORIGIN      
        1 gcgcggttgc ggtcaccgag ggtctggcca aaaagattcc ggctgcgcgc ttcgaaatca
       61 tcgacgccgg ccatttcatg ccgaccactt cacccggcgc gttgctcgcg ttgctccagg
      121 atttctggaa aagcgcctaa cgatgaaaca ccaaggacac gaaggagaca ccaagagcac
      181 gatgtctttg tgacctttgt ttccttgcct ttgtgtctct cttggaacgg tgaacatgaa
      241 aatcaaagcc aacggaattt cgttcaattg ccaggtcgat ggacgcgaag gcgcgccctg
      301 ggtggtgttc tccaattcgc tgatgacgaa tttgtcgatg tgggacgaac aggccgccgc
      361 gttgagcggt gattttcgcg ttctgcgcta cgaccagcgc ggccatggcg gcagcgatgc
      421 ccccccgggc gcgtatagct tcgacgtact gaccgccgat gcggtggcgc tgttcaatgc
      481 gttcggtatc gcaaaggccc atttcgtcgg gctttccatg ggcggcatga cggcggtggc
      541 cctcgccggg caacaccctc atcgtgtgga gcgattggtc ccctgcgatt gcggaccggc
      601 ctccacgccg caatccgccc agcaatgggc cgagcgtatc gcgctggcgc aggaaaaagg
      661 catggaagcc ttggttgaac cgaccgtcgg ccgctggttc gcagcagagt ttttgaacag
      721 cgacaaagcc gccgtcgata aagtgcgcgc catggtccgc accacgccgc tgaacggctt
      781 tgcgggctgc gcgcaagccc tttccaattt cgatctgcgc ccgaatctcg cgggcatcaa
      841 gcacccgacc ctgttcctct gcggggcgaa ggatgccgcg ctcggcggaa cgaaggctct
      901 gaacgccggc gtcgcgggtt cgaagctgat ggaaattccc gatgcgggtc acatctcgaa
      961 catcgaaaat cccacgatgt tcacgcgcgc gctgcgcgaa ttcctaagcg cgccctaatg
     1021 ttgcaggcct gtgccaaaag caaacgcggc tgcgtgtagc agccgcgttg tcacgatcca
     1081 taatcagcgg tggtgaccgc cgccgtgagg accagatccg ccgccgcgag gaccggcacc
     1141 cccgcgcgcg tgaccggcga agccgccacg gccaagcccg ccatggctta cgaaccggcc
     1201 cctgtcatat ccacgatggt tggcgtaccc gccgcgatag ccgtagccgc cccaataacc
     1261 acccacaaag gcgggccccg catatgcagg cccataatac ggggcatatc cgcaatcata
     1321 tgcgtcgtaa taatcatagc aggatgggcc gtagccataa ccatagtaag gcgcaccgta
     1381 atatgccggt cccgcgggca caccgatacc tatgccgaaa ttgacaccgc gtgcttctgc
     1441 tggcgccgcc gcagcaatgg cgatgccgca agccatggcc gcaccaatag cgaatttcgt
     1501 gaccttgttc atgtccggct ccgaagtctc tggcgcgcgt tcaacgcgct ctgtttcgcc
     1561 ctctatatag gatgccgacc tgaacctgag cttcgaggct cattcatgct gcgttcatcg
     1621 cgtgcaaaac gactgcaatt cctgggtttt ctgatacgcg aacgaatcgg aatttccgat
     1681 tacaagttcg tgagccccga agctccctgg cggcttaacg cgggatatat ttgaaatagg
     1741 gttcggcttc tttcagcacc cgcgcgtaag agggccgcgc cttcagccgt tccaggtagg
     1801 cggccagatt tttgtgcgct ccgaacggga ccgccatgct gccatagaac agcgccggtg
     1861 cggcggcgca atcggccatg ctgaaatcat cacccatcgc ccattgcttt ccggcaatac
     1921 cggcatcgat gagacgatag gctgtcagta tctgctgtgt cgcttcttga acaccgaagc
     1981 tgtcattttg tccggacggc cgcagtcggt cggtaatgat tttctgcatc ggcatatgca
     2041 catgaaggtc atagaaccga tcttccagcc gcgtgcgcca cgccgcctcg gcctcttgcg
     2101 gcacgaggcg cgcgcctttc ggatagtgcg tatccacata ttcgatgatg acgctcgatt
     2161 ccggaatcgt cagccccttc gcctcatccc gcagcaccgg gaatttgccg atgggccaga
     2221 tcttcttgaa cgcagcggac gattcctcac tggagagatc caccaggacg ggctcgaacg
     2281 gcacaccgtt ctcataaagc gcgatgagga ccttgtgaca gaaggaggaa agcgggtggt
     2341 agtagagctt cagcgacacg ggggatctct cctttcgtca gttcttgctg gcgcaattga
     2401 acatccaatg gatgccgaaa cgatcggtca gactgccaaa aaatccgccc cagaacatct
     2461 ccgccaatgg catctctatt ttaccgccgg ctgaaagcgc gttgaacaga cgcgtcgtct
     2521 cggcgcgatt gtcgggctcc agattgatga acatgttgtt gccctgcacc agtttgaaac
     2581 ccatggactc cggagcatcg gtgcccatga ggacatgccc gccaaggatg ggcagttcca
     2641 catgcatcac cagatccttg tcggcttcgg cgagcggatg gccgggattc ggtggaatat
     2701 cgcgaaagcg cgcaatgggc atggtgaatt cgctgccgaa tattttcttg tagaacagga
     2761 acgcttcttc ggtcgaacgc ggaaaattga gataggtgct tacacgagcc atgtcgcatc
     2821 ccttcggttt ggatcattca cattgatgag ggctggcctc acattttggc cagatattct
     2881 ccgagcttgc ccagcgtctg attcatgccc tcggtggcgc cgaatttctc gaccacgaaa
     2941 tcgcgttccg ctttggtgcg gaagaccgaa cgcatggtga gcgtggtctt gccgtcctgt
     3001 tcagcaaacg tgacggtcac gtggaattga tcgggatcgc tctcgtcgcc atgcacataa
     3061 accagccgct cgggcttcac cacctccagg aactggatct tgttcggata gtcggtgccg
     3121 tcggggccgt gcatgatgaa tcgccacacc ccgcccggct tcacattcat ctcggagatg
     3181 gagttggtga acccgttggg accccaccat tgcgcgacgt gtcgcggatc ggtccaggcc
     3241 tgccagacga gcgtgcgcgg cacattgtag atgcgggtcg ccccgatttc gcgatctgcc
     3301 gtgccggtgc tgtcgtcatt ttttacggtc atgctttttc ccctctactg gcggtttggc
     3361 ggcttgcagc ttgatgaggt aatcgcccag ccggtcgaga cgcgcatccc acatccggcg
     3421 atagtctccg agccagtcgt cgacgtcctt gagcgcagcc ggttcgatcc ggcagggacg
     3481 ccattgcgcc tctctggagc gcgcgatgag gccggcgcgc tccagcacct tgaggtgctt
     3541 ggagactgcc ggaaggctca tgtcgaaagg ctcggccagc tccgtgactg aggtctcgcc
     3601 gagtgcgagg cgcgcgagga tggcccggcg cgtgggatcg gcaagagccg cgaatgtggt
     3661 gctgaggcgg tccggcgcca tggctttatt gcccattcag ttaaataacc gattggttta
     3721 atacgtcatg gagagccgat tgtcaaccgc gcccccggcc tcatcattaa cccaatgctt
     3781 tcattggaaa tttccgctct agaagggcgc attgcaaagc ccgccgtccg cgcgataggt
     3841 tgcgccaacc atatttcgag cattgtgagg agagtgccat gtccgtcgtt gccaaggaaa
     3901 atccgggccg cgccagcgcc gccgaacggg tggaagagat gaacaccgag accgccaagc
     3961 tgcacgcctg gatccgcacc caggccaatg cgccggcaca gcgcccctgg ttcaccgata
     4021 tgggcgagac cggaggcacg cttgccaagg gccgtgtcgc cgccaatcag atgaagatgc
     4081 tggcccatcg ctggcgctgg cgcgaaatct atccctattt gcggaaaatc tcggagatcg
     4141 cgaccgaggc caaaggcgag cttctggagc gtaccgaccg ccagggtatc ctgctcacca
     4201 atcccggtct cggcgggcgt ttgcagatca ccaataccat ccgctgcgcc gtcgcgatct
     4261 ataatcatgg cgacatcgcg ccggcccatg tgcacagccc caatgcgagc cgcaccattc
     4321 tctccgacaa gggcggctat accaatgtcg agggcgagcg ctgcgaatgc cgccggggcg
     4381 acatcgtctt cacgcccaat ggcacctggc acgatcacgg caatgacgat cccgatccga
     4441 tgatctggat cgacatgctc gattggccgc tgatggaata tctcgattcc gtctgggtgg
     4501 atcaggatta catcgccgcg acccagagca ataccaaatc gcaggccacc aaatatgccg
     4561 acggccattc gacccgcatg tacggccatg gcgggctgaa gccggtgttc gcgtccaatc
     4621 agcgcggctg ggggcagaag tcgactccga acttccatta caagggcgtg gacgtgatgg
     4681 acgcgctgac cggcctccgc ggcgaggagg gcgacccgtt cgaaggcatc aagatgcaat
     4741 tcgtcaatcc ggtgaccggc ggtccggtct ttgcctcgct cgattattgc gcgcaattgc
     4801 tgcgccccgg cgaagaactg gcgttgaagc gcgagacctc gtccaccttc atcgtggtga
     4861 tggagggtga gggcttctcc gagatcggcg gccagcgctt cgattgggag aagaacgata
     4921 tcctggcggt gccgaatttc ctgtggcggc gccatgtcaa caccggcaag aacgacgccg
     4981 tgctctatac ggtatcggat gcggcgttga tgcgcgcgct cggtcaatat cgcgcgcaag
     5041 gcaaaaccgc cgacggctcc gtgacgcaga tcgagcggtg acgcgtgtcc cttccctttg
     5101 aggggaaggg gagaagctga cggaggtgcc tacggttcgt ccacgatgcg gttgcgcagc
     5161 gtgccgatgc cggaaatttc cacctccagc acatcgcccg ccttcatcca caccggcggg
     5221 ttgcgccgcg cgccgattcc ttcgggcgtg ccggtggcga tgatgtcgcc tggcgaaagc
     5281 gcggtgaacg ccgagcaata ggcgatcagc gccggcacat cgtgcatcag catgtcggtg
     5341 ccggaatgct gcatgacctg cccgttcagc cgcgtcgtca gcgtgagctt ggagggatcg
     5401 ggaatttcgt ccgccgtcac catccacgga cccaaaggcg aagtcatcgg aaaattcttc
     5461 cccgtgatca acgaagtctt gatgaaatcg cgcaccgacc cgtcgcagaa gcaggtgtag
     5521 ccggcgacat aagagagcgc gtccgcggcc ggtatcgcgc ggccgccctt gcccatcacc
     5581 accacaagct cgccttcgaa atcgagctgc gtggaggctt tgggccgcca catctcgccc
     5641 tcatggccta gcaggcaatc ggtgaatttg gtgaagatgt tgggcttctc cgcgaccgca
     5701 tggccatgtt ctgcagcgtg gctgcgataa ttgatgccga cacagaggat cttccccgga
     5761 ttggcgatgg tgggcaggta tttcaggcca tcgagttcca tgtcgggctc ggcacctttg
     5821 gcaagatgca gcagctcgtc cagcaagccg tcctcggcaa gcgcgtcctt caaaccgatg
     5881 ttgcgctctc ccatgcggtc cgaaagcgta acgatgccgc cttcggtaac ttgaccgaag
     5941 gcgggttttc cgcccgcgat atagctagcg agcttcattt cttcctgccc ttcgcggtgc
     6001 gcgttttctt cgccggcttc ttggcggcgg ctttcttcgc gggtttcttg gccagttttg
     6061 cgcccttctt cgacacggca acgcccgcat cggggcgcgt gaccatgtcg ccgacgcccg
     6121 ccttggtcgc ccaataatgc caggcccgcc aggtctcgac cgaatagggg ctatagccgc
     6181 gattgcgcgc gtcgatctcg ccctgacgca gatatttgac gtagccgccc agcgccttgg
     6241 ccttttgcag cgcgcgcgcg ttgcgcggca ggtacaccga catgcgcacc acttcgatca
     6301 gcgaacgcga ggcggccgcg aaaccatgac cgcgcatcag cgcgacattg ttgttggcga
     6361 ggcatttcgc gaggtcctgg ccctgctcca cattggcgac cagcagattc gtgtcgccga
     6421 atttgtcggc gatgtcccag atcggaaccg ttgagccgat gaagcttccc gaatggatca
     6481 ccggcttcag cggcgtgcca ttggtgagac cgaagggcag cacatcctcg gcatgggcgt
     6541 gcaccaccgc catcacatcg gggcgcgctt ccaagatgcc gccatggatg aagcgttcga
     6601 gatagggcga cggaccaccc tttttcaccg gctcgccgtt cagggtgaat tccatgatgt
     6661 cgtcggcggt gatgaattcg ggcgccagcg agcgggcgag aaaaaaccgc gtcggatcgt
     6721 cggggtggcg caggctgaca tggccatagg cgtccaccac gtcctcattg gcaaggatgc
     6781 ggttggcaat gacaagatcg ttgatgagat ttttcaaatt cgatttcggc atcgatgcct
     6841 ccgattgcgc gtttgcgaaa atttctagaa ccaggaatcg acttcgccgt aattgcggac
     6901 gaacagatcg tccactttcg gcgcatcggc gatcaggtgc tgatcggcga gataggtgac
     6961 gagcgcttcc agcgtcgggc gattcttctc cacgccatag ggccagggat cgccgccgaa
     7021 gacatcgtgg atttcgtcga tgtcgcgcat cagccagggc gtcatgtagc gcagcgcgcg
     7081 tagattatac attttcttga gcgcgatgcg cttggagcgt tcgaacgcgt tgtagagact
     7141 ggtcgccacg aacggatagc gctcatagat gtcgcgccga atcgccacaa gatgcatgat
     7201 cggatagatg cgcgtgcgct tgtaataatc cttttcctgc tcgacataat cggggaacag
     7261 gcggccgata tcgggatggg tgcgcaccga ttccggcagg ctggtgccga gcgtcgcatc
     7321 gacatagcct tccatgatca aatcgttcaa agcgcggccc ttcggcgctt gctcgattgc
     7381 aatgggcttc aacagcggca gcacgtgagg atcaccatgg ctgccggaat gattcaccgc
     7441 cccctgcacc caatggatct tctcgcagtc gacgccatac tcatgctgca gcaaaccgcg
     7501 aatgaagatc gccgccgtca tggtgtagag cggcacaccg acgcgtttgc cctcgaggtc
     7561 cttgggcgtc ttgatgccgg acttcttgtt gatcgcgata aacccgtggc gaaagcagcg
     7621 tgacggaaac accggcaacg ccacgaacgg gcattgcttg ttggcgaagc gctgaacgaa
     7681 ttcggaactg gaatattccg cgacgtcgaa ttcctggccc cccgacatgc ggtcgaagat
     7741 cggacgcggc tcgtcgatag cgatgaagtt gagatcaatg ccgtcgggct tcacttcgcc
     7801 cgtataaagc ggctccatgc ggtcatagag cgcacaggcg aaactcaatt gcagatttcc
     7861 cacgatcccc taccctgctt atcgtctcat gcttcgacaa gctcagcatg aagaatagat
     7921 tttatcctca ccctgagctt gtcgaagggt gaggcgaacg tctaaatctc gtcgacgacc
     7981 gaattgcgca gcaccccgat ctccggcact tcgacctcca cgacatcgcc cggcttcagc
     8041 caggcgggcg gggtctgatg accgcccgcg cccaccggcg tgcccgtgaa aatcagatcg
     8101 ccgggcttca gcgtcgcgaa ttgggtgatg tagtttatca gccaggcaaa accccaggtc
     8161 agacgctccg tgttgtcgtc ctggcgcagt tcgccattga tgcgcgtcat cagatgcaac
     8221 ggctttcccg gatcgacttc atcggccggc acgatccacg gacccaaact tcccgacgca
     8281 tcgaaattct tgccttgcgt cacgttcagc gtgccgtgac gcagccagtc gcgcagcgtg
     8341 ccctcattgc cgagcgtcag gccatagatc atcgaaagcg catgctcttt aggaacctgg
     8401 cggccttcgc gtccgatcac cagcacgatc tcgccctcgt aatcgaattt ctccgacact
     8461 ttgggccgca cgagattctg tccgtggccg acaagagagt ccgggaaccg cacgaacaga
     8521 ttgggatatt tcggcttctc gcgaccgtcc ttatattcgc ccgcgcgttc gggatagttg
     8581 atgccgacgc agacgatctt ctccggcgcc aaaagcggac gcaggaattg cacctccgaa
     8641 agcggataat ccgcaaaggg gaaagcgcaa gcagcacggg catccttcaa ccccgcctca
     8701 cccgcctgga tcagcgacaa cagcgaggga tagcgccccc ccagccgccc gccgagatcg
     8761 atcattcccg catccgtgac cgcgccaaag ctggtacggc catgaatgct gaagctggtc
     8821 agacgggtgg cggacatggg gcgcgaaact agcgggaaaa acccttgcgt caagcgcagc
     8881 tttgggttgc ggacgcgttc aatttgacgc gccgcctcgg atggctattc tgccctcatc
     8941 ctcatcatct ttaggaaaga acgtccgatg cacgatgtag cagcaaaggt ccattggcat
     9001 gaaccaacgg ggggcaaacg ggccggcttc ggcaaatggg gccgggtaaa gacgccctac
     9061 gacgccttca tggagtccga aggtatcccc tgcttccgcg atatcggcat tgccaaggtc
     9121 cagaacctgc cactcgtacc ctggaagcgg ctgggcggac gcgggaccta tatccagctc
     9181 cacggtacgg aatccaaatg gggctgctat ctggtggaag tgcccggacg cggcgctctc
     9241 aaccccgaaa agcatatgtt cgagaaaatc ttcttcgtgg tggaaggacg cggttcgacc
     9301 gaggtctggc aggacggcga caccaaacgc cacaccttcg aatggcagca gggctcgctg
     9361 ttctccattc cgatgaatgc ctatcaccgc atcgtcaacg cggcgtccgc tccggcgttg
     9421 ctgctcgcag gcacgaccgc gccggtggtg ctgaacacgc tgcaatcggt ggatgcggtg
     9481 ttcaattccc cgatccagtt ccgcgatcgt ttttcgggcg cggacgatta ttacaaatac
     9541 aatgacgatg tggagcccga tccggtgcgc ggtctcgcca tgcggcgcac caatctggtg
     9601 ccggacgccg tcaattgcga tctgccgttg gacaatcggc gctcgccggg atttcgccgt
     9661 gtcgagccgt tcatgaccgg caacagcttc tatttctgga tcggccagca cgaaaacggc
     9721 cgctactcca aagcgcacgc gcacacgtcc gccgcggtac tgatctgcat caagggcaag
     9781 ggctataccc acacctggcc cgaacggctc ggcaccacgc cctggaagga cggccatcag
     9841 gatcaggtca agcgcatcga ttacgagccc gtcggcatga tctcggccgc accgggcgga
     9901 gcgcgctggt atcatcagca tttcggcgtc tcgaaagaac ccttacgcct cttggcctgg
     9961 ttcggcccgc acaatccggg ccgcgagccg gggccgccgg gcgaacagca tatcgactat
    10021 accgcgatgg acattcccga aggcggcacc tccattccct attggatgga agaccccaag
    10081 gtgaaagccg attgggaagc caagatcgcc gaaaatggcg tggagagccg gatgctgccg
    10141 gaattcttcg acaagaattt caaaggcgcg ctgcccaagg gcgtgtgagc gtcacgggcg
    10201 cgagatgcgg agaataaaga agggaaacac aaaggacaca gagggggcac gaagcgcata
    10261 gagtttgtgg cctttgtggc tccttcgtgt tcttcgtgtc ggcatttttc tgcaaacttc
    10321 gcgtgagatt cttacgatga cgctgatcat caacaatgac gatgtcgcca aggtgctgac
    10381 gatggaagac accatcgcga cgctggaaaa atcctatctc gacatcgcgt ccaaggaagc
    10441 cgtctgccgc ccgcgcatcg acattcgcat tccgacctcc gacccggcca agaattacca
    10501 atggggcacg atggagggcg gctcgaccgc tggctatttc gccatccgga tgaaatccga
    10561 catcgcctac gagaccgtct acaatggcgt gcgtacgcag gaaaaatatt gctcgcgccc
    10621 cggtttattt tgcgggctcg tgttactgac ctccatcgaa accggcgagc cgctcgcctt
    10681 catcaatgat ggtgtccttc agcacatgcg ggtggggggc gatggcggca tcggtgtgaa
    10741 atatatggcg aacgcggatg ccgcgaccgt cggcatgttg ggctccggcg gcatggcgca
    10801 cagcaacatg gaagccttca tgacggtgcg gcctatccgg cggctgcaaa tcttcagccc
    10861 cacgaaagag aaccgcgaac gctttggacg tgagatggcg gaagagcacg gcatcgacgt
    10921 caaagtctgc gaccgcccgg aggagattta cgaaggtgcc gatatcgtcg cggcttgcac
    10981 cgattccgcc gtgccggtgc ttgacgggac tcgcgtcgag aaaggcgcgc atgtcatcaa
    11041 tatcggtggc tccggcgtac ccgatgacga aacgctgaaa cgcatcgacg tttacttgcg
    11101 ctttggcgat gcgccgccgc catcaagccg ccccggcctc gccttcgacg acgagcatat
    11161 cggctgggag gcacggcccg gtcagcccaa gcatggcgat ggcaggggcg ttcgcaagcg
    11221 cggccatggt gtgctgctgc ccgaaaaacg catcagcctc gccgatctcg tgcagggtaa
    11281 agtcaaaggc cgcaccgcgc gcgatcagat cacctattcc gaacgcggca atctgcaggg
    11341 cgcgcaattc ttcgccgtcg cgggcgttgt ctatgacatc gccaagcgcg aaggactggg
    11401 acacaaaatc ccgaccgaat ggtttctgca gacgatccgg aactgacgca ctaaaggcaa
    11461 ttggttgccc tgcgtacgag tatcaaaatc gtatccttca cgaatgccgg atattcgcga
    11521 agctgaaacc acagccattt ctgccacgga ttcgctgcac ggccgctcgc cgcgccgatg
    11581 ccgcctgccc gaatgccgct aaggcgtaaa agccagcggc agcgggccac atgataggca
    11641 tcgctgcaga ccatcacacg ggtgatatcg ggcaggccgc gaatgatcgc ggtgcagcgg
    11701 atcaccgatt ccaacgtcgt tttcgattga ttttcctgaa cgatcgcgtc ttccggcacg
    11761 ccttcggcgc gcagcatgtt cgccatcacg acggattcca ccggtggata gattcccaat
    11821 ccccccgtca ccagaaaggt gacagaccgg tcgcgcccat aattcaacgc agcttcgaca
    11881 cggcgccgca gcgccccgct cggactgcca tccgcacgaa ccgcggcgcc aaacacaatg
    11941 acgacgtcag ggccctgtga catcaggagg gaagacaaat tcccgcgggc gcatcgacgt
    12001 ttttgttgag cggataaacg aacgtatcgc ttcgaaccac tttcgatccg tttggaagac
    12061 gttgagcctc cgcgacgacc gaggctttga attccttcgc cgtcgcttgg tcgaaggtga
    12121 attggatttg cccggtcggc gcgatgacgc ccaaagccgt gccgaaggac ggcgtcgtca
    12181 aaggcggacg cacaaccccc acaacgcgca taccccggaa tgtgaggttc atcctgtcca
    12241 cctgctgttt ggcggtgttc acgcgcaggc gcgcattgtc caattgcggc ttcgactgca
    12301 ggtcggacaa ttgggccgaa ttgaccagaa cctcgtaatc ataggcgttg tcgggacgga
    12361 tatagatgcc cgtccaggtg aactcacgcc ccgctgtctg aaccttcaca ggttccgcat
    12421 tgcgcagcgc cacatagctg cgtttcaacg caatcggcgc acccgaaatg cggccatcgg
    12481 aaaccggctg gagtttcgct tcgatcacgt cgcagccgct gttgatacct gcgaaatcga
    12541 gattctgcag cgcgaattcg acatccaggc tgcgtgcgct tgtgtagggc agaacgcggt
    12601 attccggatc gactccgtcg atcctgacag gggtgccatt gactttgagg tccagcacga
    12661 cgctgtcgat tttctcaccc ggcaaggacg gcgcctcgat gtggaattcc gtaaagccgg
    12721 cgccgcgcaa atccttgcgc ccacgcaagg tttcgagcga cggcacattc catacctttg
    12781 tcggcgtttc ttcggcgtag gccgtggaga tcagtccggg ccaagacagc gacgtacttt
    12841 tctccggcgc gcagaaactc acccagccgg gatcgtcccg gtctttcaat ttgcactgac
    12901 cggatgattc attgcagtcg agcactgcat acagatccgc gcctcctgga ggagacttgc
    12961 cgtcgacgag gcatatctcc gttcccttgg acgattcgac gcgatagcca tcgcgcacaa
    13021 cccgatgcag caccgcactg tagggatgct gcttcgacgg catcagcacg ttcggccagt
    13081 catagacgga gtcgggaggc accgcgagcc ggggatcggg caccagatat ttgaactgag
    13141 cgcttctgaa tttttcgccg atgaaaatcg cggcggaatc gtaaaccgga tttttggggt
    13201 ccatgaacgt cagagaaaaa cgaatatcca actggcgcgc cggcgaatca tccacggcct
    13261 ttcccgtgat cagcgcgaaa atctgattgg tataggtttg catgcattgc cggatatcgt
    13321 cattttcctg cagcttaagg gactcgttca caacatcgac cgcgcccttc aaatcctcca
    13381 cctgggtcgt ggcgcttcct ttgccgctga gcttgtcgac ggccgccgcc gcgtcgatgc
    13441 ttgcggttcc cgacaatttc tttccggaga ggcaggtatt ttcggctgcg ttcatcaagg
    13501 cggctacctg aacgacacgc ccgtctgcgc ccatgaccgt gggggtcgtc ctggactgga
    13561 aataatagac gccaaaaacg atgacggcga caccgcccag acctagaagc agatatttga
    13621 ccatctcaca cccctgcgac agtggcgcgg cgcatattaa ccaactgcat cggctaccgc
    13681 aaaggcaacg ccagaattca ccccatcatg tgaagcacat cgcctttgcg tttgaccggt
    13741 tcggccgcga tcaacgcgaa cgcgatcacg caaggctgcg tcccgcgatt gatccaattg
    13801 tgcaccgtgc cgcgctggac cagacaatcg ccctggttca gatgcacgaa cgtgccgtcc
    13861 atctccatgt cgatttcgcc cgatatgacg accgcgtagt cgatggaatc ggtgcggtgg
    13921 ctgcggggcg cgacacccgg atcgtagcgc acgatccgga acaccgcgcc gcccggatcc
    13981 gtggagccaa cgggacgcaa cgcaccgtcc tcatcaccgc tattgtccgc cggaaacgat
    14041 ccggttgacc agatgacgca actatggtgc ctttcgcgcc ccgatctcac attgctgcag
    14101 atctcgtcga ttttcacgat ggctttgcca tcatcatcat gaccggtgac cacacgtcgg
    14161 atattccatg acatcaggag gttccactaa ggcatttcgc gcatcgatct tttatggtgt
    14221 gtggcatcca agcgcaaagc aaagcgaggc gagccgtgga tagcgaagac aagaacgacg
    14281 gcgtcaaagg caagatcctg caatggccgc tcgcgcgagg accgctgagg gtccgccgcc
    14341 agccagcgct tgcgttgcct gcgattgccg ccggcgcgtt ggcaatcggc gcattggcca
    14401 tcggcgcgct cgcgatcggt agtctcgcca tcgggcggct ggctatcggc aggacacggg
    14461 ttcagaagat ggaaatcggg gatctgacgg tgggccgtct gcgcgtgctg gagcgcgacg
    14521 attaggcatc cctgcacaat tcgaaattaa agaacgctat aggagacaac aatggaacga
    14581 cggatattct tggcgggcgc ggcgggcgcg atggcggcgg ctgcgctacc ggaaatggca
    14641 aaggccgatg aattgccgct gggccccttg ccgggcacac gttaccccga atcccgaatt
    14701 caagccctgg acccgcggtt caaatatcgg atcggaaacg cttatatcga acgggtggca
    14761 acgaattgcc gctggtgcga gggcccggtc tatttccgcg atggcggcta tcttttgtgg
    14821 agcgacatcg ccaacaaccg catcatgcgc tggagcgaag atgacggcca tgtcagcgta
    14881 ttccgttcgc cttccaattt cgccaacggc cacacccgtg atcgcgaggg ccggctgatc
    14941 tcctgcgaac acgatacgcg gcgcgtgacg cgcaccgagt atgacggctc catcaccgtg
    15001 ctgatggaca aatataatgg caagccgctg aacgcgccca acgatgtggt ggtggcgtcc
    15061 gatggcgcca tctggttcac cgatccgggc ttcggcaagc tcagcaatta tgaatcgacc
    15121 ggcaaagcgg agctggaatt gccgccgaac gtctaccggc tcgacccgcg cagcgggcgc
    15181 gcaacggtgg tctatgttcc cgatgccggg cgtccgaacg gtatcgcctt ctcgcccgac
    15241 gagaagaagc tgtacatcat cgacagcgcg attccggctc cggcctcatc cgacatccgt
    15301 gtgttcgatg tgaacggcga caaaatcgtc aacggaaaga tattcgccgc cgacttcacc
    15361 aaagggtcca ccgacggcat gcgcctggac gtggatggaa acgtctggtg cagcatgggc
    15421 aatggcgatc cggctgaaga cggcgtgcgc tgctatgcac cgaatggcga tctgatcggc
    15481 aagatccatc tgccggagac ctgcgccaat ctgacttttg gcggcaaaaa aaagaaccgc
    15541 ctattcgtct gcggcagcac gtcgatctac tcggtctata tcgacacggt cggcgccatg
    15601 gtgccgtaac ggctcacaag cagcaggcgc gttttcagaa ccggttaaag ccgccgaaac
    15661 cgccggcgat gtcgcaatgg tcggagaact cgttttgacc gggacggatc accgggagtg
    15721 gggctccaac cggcgccccc tccggcgagg cagcaacctg ccgcagcagt ccgttttctc
    15781 caggcatccg atccgcgaat ttcgtcatcg gatcgattcc ggaaatctcc aggatcagct
    15841 tgtgccgcat ggccaagccg aaatccctga agcttttcgc cacgaccacg aaggaggcgc
    15901 gtccgccagc gacgcaggcg gcgtaatatt tgtcgagacc ggggaaatag ccgttcgcgt
    15961 tatcgtccat gatgggcaac ccgttgacga cgataccggt gccgatgacg cgatcatgga
    16021 cctcgcgcat attctcgccg tcattattcg ggccatcgcc ggacacatcg atgacgcggc
    16081 gcgtggcgac aatgtccttt tcggtcgctt cgatcatcgt atagccgagt tgaagcgcgc
    16141 tgctgaccga agtgcgacgc ccgggagagc ggggcgcgcc gctgacaagc tcggagaagg
    16201 cctgggcgct cgctttgtcc ttgacgatgt gccagtcgat gaccattttg gcgaattcag
    16261 ggctcgagaa atcgaacatc gcgaccgcga tacgccccag ggcaccgctc tgaattgcct
    16321 taaccacctc tggatcggca aaggccgccg ccgttccctg gcgctggagg tcgaattcct
    16381 cctcattgat gctgccggag acatcggtgg caagcaccaa cttcaaatcc acctgctcgc
    16441 tgccgctcgc cgcgggagcc gacgacgaca gaccgaacaa agcgagcgcc gaaaccgtga
    16501 gaagcatcgc gcgcatcggg ctgcgggatt gtctgtgact tgcagaattc gtcatcgatt
    16561 gacctcccgc tgatgcgatg cggcccgtaa gggttgcatc ataaccggca acagacgtct
    16621 acgcgtctaa ccaatccgtc atttcgccga cgtgaacgac aatacctaga atttgatcat
    16681 ggtgcttcgc gtcaaggtga cttggctggt cacggccacg ggaccgccct tcgccagatg
    16741 aaaggctgca tattgctcgt gataggcgtc ctcgccagcc gagcagtcca ccgggacgat
    16801 aaccttgtaa ccgcgttggg ccgcgcccga cgccgtaccg acggtcgccc cttggaacga
    16861 gttgccgcag acgatcaccg tcttgatccc ggcttgccgg agagttgcct ggagcgtgga
    16921 gcccaggaat ttgtctgggc caccctggcg ataccattcg ccggcgcgcg gcttgatcgc
    16981 ggcgtccatg atgtcgtcgg gcgtcgcctt gccgttagac ccgacgagac tgtaccagac
    17041 gaccatatta tgcgagcgcg cctcctcgtg cagccgtttc atgttcggta ccgcggcgat
    17101 gcagcgtgga cgggcggagc agccgccttt catgatatcg agaaacagaa gtgcggtggt
    17161 cgaaggatcg accgtgacct ctttcaattc cggcggcgga ggtgctttgg ccgtgttcca
    17221 atcggcgatg atgtccgcac cggcaggcga ggcgagaata gccgtaaggc cgaatgcacc
    17281 cgcaatcgca cccatgacaa ttttcgtagc catcgaaacc tcccgttgag cgtgatggga
    17341 acaggaaagg cggtttgtgc tggcgtaagg atagagccgg caggccaatg gcgccatagg
    17401 ctttctggaa aacccaacgg gaaccaagcg agagggcgtt gcgccgcaag aaacaggagt
    17461 accggtccgc cacaggccaa tacgcgaacc gaagttttcc cgcgtgaaac cttgataatg
    17521 acggtcttta gcccgtcaat cgtttgggcg atccggttgc gtggttttca gggtttcgag
    17581 aatggtccgc aattcggcaa tccggacagg cttggaaacg atacccgcca tatgcaaccc
    17641 gcgatcttcg ccaagccggc gcgcaccgtt caggatgcgg gcatcgaaac cgctcaagat
    17701 caatatccgg gcacggctct tttctgtcgc aaggaacttc agcaattcga ttccgtcaat
    17761 gtccggcatg gacaagtcga gaatgacgac gtccggatcg agggaacgat gagtccgctt
    17821 gaactcatcg gccagcgtca caaaacgcat ctcatagtcc agaccttcgg cgacccgtcg
    17881 gacaagcgcg ccgatatagg gctcgtcatc gatcatgagc aagcgcgtca cactcatgac
    17941 agcccatccc tgctctgaag agtctctagg atccggcgag caaggtccgg ttttcggtag
    18001 ggtttgctca agagattgtg gccatcgatg tccggagaac ggctcccgtt ctggacagag
    18061 gcctccgcaa aaccggaggc aaacagcacc ttcagatcag gccgcagccg tttcgcctcg
    18121 cgcgccagca gatcgccagt catcgcaccg ggcattatca cgtcggtaaa gagcaggtcg
    18181 attgtcctgt cctccagaag aatcgtaaga gcgccgtccg cgttcgcggc ctccagcacg
    18241 tgatatccga gttcgccaag ttggcgcacg gcaacactgc gaacatcggc attgtcctcg
    18301 accacgagaa cggtcgccgt tcctaccagg gtcttcagtt cagcgacgcc ttccttaggc
    18361 aattccatcg cggcatttgc ccgtggaagg tagagacgaa tggtcgtgcc atgtccgatc
    18421 tcgctgtaga tcttcacgtg gccgcgcgat tgcttggcga agccgtagat catactcagg
    18481 cccaaccccg atcccttccc ctcggccttg gtggtaaaga acggttcgaa gacgcgatcg
    18541 agaatttccg gtggaatacc gctcccggtg tcggaaacag caagcatgac ataattgccg
    18601 gggacgacct ccgggttctc catctgatat tgctcgtcga gatatttgtt cgcggtttcg
    18661 atggtcagat gtccgccttc gggcatcgca tcgcgcgcgt tgatcgccat gttggcgagc
    18721 gccgattcca cttgcgtttg gtccgctagc gcaggggaca ggtcatcggc caaccgcatt
    18781 ccgatttcta tccgcccacc cagcgtccgg cgcaaaagct cggtggttcg cgacacgact
    18841 tcattgaggc aaagaacttt cggttcaagc gtctgacggc gcgaaaacgc taggagctga
    18901 cgagtcagat cggcgccccg caggctggcc gtgagcgcgt cgtgggccat ttcccgcgcc
    18961 ttcgaattgg gcggaagctc ttcctccagg aggtcgagat tcccaacaat gatcgccagc
    19021 agattgttga agtcgtgcgc gataccgccc gtaagttgac cgacgccttc catcttttgc
    19081 gcctggacca attgtttggt cgtgcgcttc atctcctcta cgttctgctg aaaagcatgc
    19141 aaaacgatgg acaatttgcc gatttcatcc tgccgccccg tgggaaggac aggcgccacg
    19201 atttcgcccc gcgtagcctg caaaagagca acgaccatct ggttgatggg acgaatgacg
    19261 cgcccgatta tcacccagaa gctatatgcg caaagcaaga gcgcaagcag aaacaccgtc
    19321 acctgcagcg tgatcccctc gaccgtctgc gcgatgaggc cttccacgta gctccgcgtt
    19381 tgcgcgatcg atcgattttt gagcgttccg agcgaatccg aagcctggtt cgacaactcg
    19441 aaccagagat ccgcgctgat cggatattcc gcaccctgcc ccgcgtccgg ggcatagatc
    19501 atgccctgga tcatgtcgtg aatggtggag taatgactgc tggcgtcggt gtaggccggc
    19561 gcaatggagg cgtagaggcc gctttggccg gccaggctgc ggctcatcga ccagcttgcc
    19621 tcgacaattc cctgcccgcg caacaattgg gcaacctgtg cgggagtagg cgtggcattt
    19681 tccgcgatga gctgtcccac cagcgagcgc tcccggccgg tgtaatcggt gatggtccgc
    19741 agaagctgct tgaaccaaag atgttgcgtg acgaccggat cgatattggt gaaatatctg
    19801 acgaaatcga tccacaaatt ctcggtgtcc tgcatcgatg ccgtgacttc ggcggaccag
    19861 cgtttggaca agtctggatt tcgttgctct ttcggaagat ccaccgcgcc gtcgatttca
    19921 ggccgtaatt tctgaatgac cgcgagatgc gattccatct cggcgcgcag cttggcgact
    19981 tccgggaatg aaaattgatt gagcgtcgtc atggattcgc gcaaggattc gtctgccgct
    20041 tggcggctct cgttcaagcg cggccgcaaa tcgtcgatgg tgtcggtatc cagcgcctgc
    20101 atcatcgaga gcgcgatatc gcgttccacg ctgagtttct cgatcgcgtc aaagagttga
    20161 tcgctcgaga tggtcgcttc cctcagggcg cggatatccg ccagccgccg ccaattgccg
    20221 tacatgtcct tggccgcgag cagcacaatg atcagtgtca atgtgcccgt tatcaacaac
    20281 aggatgccgc caagagaccg ctggggtgcg accatagcta tggtcccggc gtggtcgtgc
    20341 gtaccatgct tgccagcctg agcaagcggt cctcgacctg cagcttggcg gagccgattg
    20401 gctccgtgtt gatcgtgaca ctgatccggt tggcgatccg ggtgaattcg atcatgccgc
    20461 cggacagagc gaaaccttcg aaatcgctta ctgtcagtac gcttttgccg gcaagggctt
    20521 tttgcagttc cggccagttc gctttttgat cggcacggac aaacaacaat gagcaatcgt
    20581 cgatgtccgt gacggcgcgc acgctcctga cttcgatgac gtgttcattg acggtgcggc
    20641 cagccatcgg ctgcaaatgc ccatcgaagg ggtcgccgcc aaagaggcag accttggttg
    20701 tcccttctcg ctgcgcgctt tcggggggcc attgcgtgta cttcaagaaa ttgtagagca
    20761 gcccggcctt gatctgctgt tcgacgagct gcacatccgc ggcctgcgcc ggcagggcca
    20821 gaaaagacgc cagcagaata gcgataatgg cacgaacgcg caggaccgag ggagccgcgt
    20881 tgagccatcc ggcgaagctg ctggatttta tgagacacgc tgtcttgatc gcgttctcaa
    20941 gcgcagccag cgcatgcgtt gcggtgcggt tggcaacggc gatctttcgg gcaagaccca
    21001 tcatcttcag aatgaccatc gggcgctcac gacaacgctt cttggcactt gacttgctcc
    21061 aaccgttgca ccgaactccg catgatggtc atgcaacaga ttgaatcctg ctatcgc
//
LOCUS       DASYEG010000186         8941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4699085, whole genome shotgun sequence.
ACCESSION   DASYEG010000186 DASYEG010000000
VERSION     DASYEG010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 8941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8941
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4699085"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VGM72_08495"
     CDS             complement(<1..956)
                     /locus_tag="VGM72_08495"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY4265343.1"
                     /translation="MLQMWLGGIEGLLQLRELAFLGLGMLIGLFFGAIPGLGGAAALA
                     LLMPLTYGLEPFTALALSGGVMGGVPMGGSITAILLNTPGSAPNAATAIDGYPLAQQG
                     KAGLAIGAAASANAIGGIIGTVSVLAILPIAKDIVLLFGPPEFFLLAILGLVIVSTTS
                     RGKLLRGLITGAFGLMVAFIGYNDVSGGERFTYGIEYLWDGVHLVPALIGLFAVAEMI
                     NLYVKGGSVSNNAASVKITSMTGGLLETFRHWPTVIRGSLIGTIVGAVPGVGGVVASF
                     LSYSMTVQAAKESEKDSFGKGNIKGVIAPEAAINAKDCSALIP"
     gene            complement(1029..2012)
                     /locus_tag="VGM72_08500"
     CDS             complement(1029..2012)
                     /locus_tag="VGM72_08500"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY4265344.1"
                     /translation="MQGFTRRELVIGAGIAAPALALPGFARGAAAFPNKNIQFVIPYA
                     PGGGFDVYVRVIAPVMEKYLPNKVNIVPINVAAGAGSRGVAQLYRAKPDGYTIGILNI
                     PGMFILQQQQGPGAYDLSKFSWIGTMGEGERYVICVGAKSPLKSFADLKALSAKRPIK
                     LSVTGPEGTAYAATMIGSQLLSLKTQLITGYKGSADYVVAAIRGDSDAVIAAIPTASR
                     FVHGGTIRVLASFESHSSFPGVPDAAALGQPELDNISVERVIAAPPGLPADIQNTLSA
                     VLAKALADPMVVKWAKENDLIMRSRTPPETTQLVAQQRIFFDKWKKYLTAN"
     gene            complement(2032..3036)
                     /locus_tag="VGM72_08505"
     CDS             complement(2032..3036)
                     /locus_tag="VGM72_08505"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY4265345.1"
                     /translation="MPHALPNTTGQVNRRALLAGGTACLVSGRVAAAAAALAGFPAKN
                     IQFIIPYAAGGGFDAYVRFVSPVMEGYLPTRASIVPINVTAGSGSRGITQLYRSKPDG
                     YTIGIFDLPGMFIQQAVQGSSAYDLSRMAWIGCMGEGERYLVGTSANSALKSFADLQA
                     LSARRAVKFAVTGIEGTATAATIIGTEVLGIRRQLITGYRGSSDYIVATIRGDSDAVI
                     SATSTMMRFARAGQLRILASFEAHSSIAGIPDATSLGKPELDQITVERPVGAPPGTPA
                     GIQNALSAALAHALADPKVIGWAKENDIVMRSKTPAEAAALVVRQRAFFDRWKKYLVT
                     "
     gene            complement(3096..3773)
                     /locus_tag="VGM72_08510"
     CDS             complement(3096..3773)
                     /locus_tag="VGM72_08510"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY4265346.1"
                     /translation="MKLYADPVSTTCRPVLMFIAESGAGVDMVHIDLFTGAHKKPEYV
                     AINPSGAVPCFEDEDLRIGESSAILKYIADKIDSPAYPKDLKKRARINAMMDWFNTGL
                     YRDFGYGYVYPQIFPHYKQEHEIAQTATLNAGRERAKKWLTVLDKDFIGPKNKFLLGS
                     EITIADYFGADILGLVDVVRCDLSPWPNVQRWLKNMRALPNWNKVHADYNAKLVTPTK
                     DAPFVML"
     gene            complement(3821..5071)
                     /locus_tag="VGM72_08515"
     CDS             complement(3821..5071)
                     /locus_tag="VGM72_08515"
                     /inference="COORDINATES: protein motif:HMM:NF005720.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-dependent oxidoreductase"
                     /protein_id="HEY4265347.1"
                     /translation="MTARTGNIIAIVGGGIAGLALALGLHKRGLASRVYEAAPEVKEL
                     GVGITVLPHAMRELTALGLSDLVTASGVVNTQSAFFNRFGQLIYKEPRGRYAGYPYPE
                     AGIHRGRLHTILFNAARERLGAENILTNRRCTGVGQDADGVTLAFQETNSGAALPPVR
                     ADIAIACDGINSVVRKQFYPGEELAFEGINTWRGVARSKPILDGHTYLRIGSIKTGKI
                     VIYPIIDNIDGNGNQLINWTTEAPLFGRPKNDWNKTGKLEDFLPIYDTWRFDWLDVPQ
                     LLKNSEILLEYPMVDRDPVPRWTFGRIALAGDAAHPMYQRGSNGSAQALIDARVLSDL
                     LAASGGDRSAALLGFEQARLEATAKIVHTNRATPPDFINIKVEELVGDKPFENLDRYI
                     SQDELKALSDNYKRVAGFGQQDVR"
     gene            complement(5068..6060)
                     /locus_tag="VGM72_08520"
     CDS             complement(5068..6060)
                     /locus_tag="VGM72_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEY4265348.1"
                     /translation="MAGIRVAVTDDWQNAAPDVADWSELRKRAEVVFFPRHYPAADED
                     RAAQELKDFDIILPTRERSRFSESLIARLPRLKMMSLTGSRAPNVDQDACTKRGIVCT
                     FTSGGHSPAPASEMTLALLLAAARKVPQGDAAMRAGRFQEGIPPGINLEGRTLGIVGL
                     GRIGAYVARYGHALGMKIIAWSQNLTDEKAKQAGAERVSREQLFAESDAVTIHLVLGP
                     RSRGLIGAADFARMKKGAIFVNTSRGPIVDEAALLAALREGRIIGALDVFDREPLPPD
                     NPFRDLPNTVLTPHLGFTTVEGLGVFYEHAIHNILAFLDGKPINVANPDALISR"
     gene            6275..6958
                     /locus_tag="VGM72_08525"
     CDS             6275..6958
                     /locus_tag="VGM72_08525"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEY4265349.1"
                     /translation="MSKRMNRANNLAQEQPPVPGAFIDLDALGAAAVAREPFAYFIVP
                     GFVKAEARDTIRTDFPAIRHPGSFPLATLHYGPAFAAFMDAIQGKAMAAAVGDKLDMD
                     LTESPTMVTVRGQSRAADGKIHTDSRTKLVTALIYMNDSWESPKGRLRLVRSQTDLDD
                     VVAEVPPEQGTLLVFRNEPNAWHGFEPFSGPRRVIQLNWVTEASVVRRELARHRLSAF
                     FKRLGNHKA"
     gene            6977..8875
                     /locus_tag="VGM72_08530"
     CDS             6977..8875
                     /locus_tag="VGM72_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265350.1"
                     /translation="MPLNIETFSNAVGGSAFYKALTHPLTADLARGLLAKLRGSGPVA
                     IYDPHNFLAAFDAMFPLESITIAGVFVQDVSHIGRTFRGHCAKALTELSACHAQAVLI
                     AGFDAKPSMQHLAPLTNGAALLSFDAFRLPDGMLSDKTRYLSTLNFATNFVFFRDAEG
                     LHTRLATANYWSAYGGKGGHLWFALFDDSGAQIAQWEEPLPAANATIVVDSRAVRARF
                     KLGPFTGQLFVHVVGAAGHDIVKYALDTYGDEDDVLSCTHDANSWPANLYAGLPAPAD
                     DEDVVLWVQNSHPNPIAPGEIGLNLMGDEHIAYLDEPVGAFATKRLSASALLPVARWP
                     QQIEIHAGKHFVRPRYEVLRKNGHRHIAHPNVEREDLKLDAQLPDLTAVLGKGFILPA
                     PVLPIERFRSMILPTPMSTVQNHLPVKLVVYDSSGRKAAEHAFGNLARNDCVALAANE
                     ILNGRIDGGYGHMELVYDFDAGRAADGWLHALFRYEDLKTGHAAETSFGAHMFNMLVT
                     YKDEPQSYKGPPPGLSTRLFLRVGAKPLDTMCHLVYPASRPWHAASDTQLMLMSARGE
                     EVARRALKIACGGSALWRVSEMFSAGERSDAGENCYIVVRDSTCRLFGYHGLIGGRAF
                     SLDHMFGF"
BASE COUNT         1678 a         2745 c         2788 g         1730 t
ORIGIN      
        1 ggaatcagcg cgctgcaatc cttggcattg atcgccgctt cgggcgcgat cacgcctttg
       61 atgttgccct tgccgaagga atccttttcc gactccttcg ccgcctgcac cgtcatgctg
      121 taggacagga acgaggccac caccccgcca acgcccggca ccgcgccaac gatggtgccg
      181 atcagcgagc cgcggatcac ggtcggccaa tggcggaagg tttcgagcaa gccccccgtc
      241 atgctggtga ttttcaccga cgccgcattg ttcgaaaccg aaccgccctt gacatagagg
      301 ttgatcatct cggcaacggc gaagagcccg atcagcgccg gcaccagatg cacgccgtcc
      361 cacagatatt cgatgccata ggtaaagcgc tcgccacccg aaacgtcatt gtagccgatg
      421 aaggcgacca tcagcccgaa ggcgccggtg atcagaccgc gcagcaattt gccgcgcgag
      481 gtggtcgaga cgatgacgag gccgaggatc gccagcagga aaaattccgg cgggccgaac
      541 agcaacacga tgtccttggc gatgggcagg atcgcgagca ccgacaccgt gccgatgatg
      601 cccccgatgg cattggcgct cgccgccgct ccgatggcca ggcccgcctt gccctgctgc
      661 gccaacggat agccgtcgat cgctgtcgcc gcattggggg cggagcccgg cgtgttcaat
      721 aaaatcgccg tgatcgagcc gcccatcggt acgccaccca tcacgccgcc cgacagagca
      781 agagccgtga agggttcgag cccataggtc aggggcatca gaagggcgag cgccgcggca
      841 ccgccaagtc cggggatggc tccgaaaaag aggccgatga gcatgccgag gccaaggaat
      901 gccagctcgc gcaactggag caggccttcg attcccccga gccacatttg aagcatgagc
      961 gcttaaccct tccggccgca cgagccggga ttttcgaggc taagcgttag tagggctgcg
     1021 cggagcggtc aattggcggt cagatatttc ttccacttgt cgaagaagat acgctgttgc
     1081 gcaaccaatt gtgtcgtctc cggcggcgtt ctggaccgca tgatcaggtc gttttccttg
     1141 gcccatttca cgaccatggg atcggcgagc gcctttgcca gcaccgccga aagcgtgttc
     1201 tggatatcgg ccggcaggcc cgggggcgct gcaatcacgc gctccacact gatattgtcg
     1261 agttccggct gacccagggc cgcggcatcc ggaacgcccg gaaagctgga atggctctcg
     1321 aaggatgcca gcacacggat agtgccgcca tgcacgaagc gcgaggcggt cgggatggcc
     1381 gcgatcaccg cgtcgctgtc gccgcgaatg gccgctacga cataatccgc cgaaccctta
     1441 tagccggtga tcagttgcgt ctttaagctc agcaattgcg aaccgatcat ggtcgcggca
     1501 taggccgtgc cttccggccc ggtgaccgaa agtttgatgg gacgcttcgc cgacagcgcc
     1561 ttcagatcgg cgaaactctt cagcggcgat ttcgcgccca cgcagatgac gtaacgctcg
     1621 ccttctccca tcgtgccgat ccaggaaaat ttggacagat cgtaggcgcc cggaccctgc
     1681 tgctgctgca ggatgaacat gccgggaatg ttgaggatgc cgatggtgta gccatcgggc
     1741 ttggcgcgat agagttgcgc cacgccgcgg ctgccggcgc cggcggcgac attgatgggt
     1801 acaatgttca ccttgttcgg cagatatttt tccatgacgg gcgcgatgac gcgcacatag
     1861 acgtcgaatc cgccgcccgg cgcataggga atgacgaact ggatgttctt gttcgggaag
     1921 gcggcggccc cacgcgcgaa tccgggaagc gcgagcgcag gggctgcgat gcccgctccg
     1981 atgacgagtt cgcgccgggt gaatccttgc atcgtttatt actccttgaa cctatgtgac
     2041 gagatatttc ttccagcggt cgaaaaacgc gcgttggcgt accaccaggg cggcggcttc
     2101 cgcgggggtt ttcgagcgca tgacgatatc gttttccttg gcccagccta tcaccttggg
     2161 atcggccagc gcgtgcgcaa gtgccgcgga cagcgcgttc tggatgccgg ccggcgtgcc
     2221 gggcggcgca cccacgggac gctcgacggt gatctggtcg agctcgggct tgccgaggct
     2281 ggtcgcatcc ggaatcccgg caatgctgct atgggcctcg aacgaggcga ggatgcgaag
     2341 ctgccccgcg cgggcaaagc gcatcatggt ggatgtcgcc gaaatcaccg catcgctatc
     2401 gccgcggatg gtggcgacga tgtaatcgct cgagccgcga tagccggtga tcaattggcg
     2461 tctgatgccc agcacttccg taccgatgat cgtcgccgcc gtcgcggtgc cctcgatgcc
     2521 ggtgacggcg aatttgacgg cgcgtctggc cgagagcgcc tggaggtccg caaaactctt
     2581 gagcgcggag ttggcggacg tgcccaccag atagcgctcg ccttcgccca tgcagccgat
     2641 ccaggccatg cgtgaaaggt cataagcgct gctgccctgc acggcctgct ggatgaacat
     2701 gccgggcaga tcgaatattc cgatggtgta gccgtcgggc ttggagcggt agagctgtgt
     2761 gatgccgcgc gagccgctgc ctgcggtgac gttgatcggc acgatgctcg cgcgggtcgg
     2821 aagatagccc tccatgaccg gcgaaacgaa ccggacatag gcatcgaagc cgccccccgc
     2881 cgcgtaggga atgatgaact ggatgttctt tgccggaaat cccgcaagcg ctgctgcggc
     2941 tgccgcgacc ctgccggata cgaggcaggc agtgccgccc gcgaggagcg cgcggcggtt
     3001 gacctgaccc gttgtattcg gcagcgcgtg cggcatgggc acaaacctac cctgaaggag
     3061 cccgcaaacc tagccgccgg tacaatcccg cccgttcaca acatcacgaa cggcgcgtct
     3121 ttcgtgggtg ttacgagttt tgcgttgtaa tccgcgtgca ctttattcca gttcggaaga
     3181 gcccgcatgt ttttcagcca gcgctggacg ttgggccatg gcgacagatc gcagcgcacg
     3241 acatccacaa gcccgaggat atcggcgccg aaataatcgg cgatggtgat ctcgctgccg
     3301 agcaggaatt tgttcttggg accgataaaa tccttatcca ggacggtgag ccatttcttc
     3361 gcccgctcgc gtccggcatt cagcgtcgcg gtctgcgcga tctcgtgttc ctgcttgtaa
     3421 tgcggaaaga tctgcggata gacgtaacca tagccgaaat cgcgatagag gccggtattg
     3481 aaccaatcca tcatcgcatt gattctggcg cgcttcttca aatccttcgg ataggccggc
     3541 gagtcgatct tatcggcgat gtatttcagg atcgcggagc tttcgccgat gcgcagatcc
     3601 tcatcctcaa agcagggcac cgcgccgctc ggattgatgg cgacatattc gggtttcttg
     3661 tgcgcgcccg tgaacagatc gatgtgaacc atgtccaccc cggctccgct ttccgcgatg
     3721 aacatcagca cgggacggca agtggtcgat accggatcgg cataaagctt catggtttcc
     3781 ccctctcatc gccgctccgt tattggagcg gcgtttttac tcaacggaca tcctgctgtc
     3841 cgaaaccggc gacgcgcttg taattgtcgg acagcgcctt gagttcatcc tggctgatat
     3901 agcggtcgag attttcgaag ggcttgtcgc cgaccagttc ttccaccttg atgttgatga
     3961 aatcgggcgg cgtcgcgcga ttggtgtgca cgatcttcgc ggtcgcctca agccgcgcct
     4021 gttcaaagcc gagaagggcg gccgagcggt cgccgccgct cgccgcgagc aaatccgaga
     4081 gtacgcgcgc gtcgatcagc gcctgcgccg atccgttcga gccgcgctga tacatcggat
     4141 gcgcggcatc gcccgcgaga gcgatgcgcc cgaaggtcca acgcggaacg ggatcgcggt
     4201 ccaccatcgg atattccagc aggatttcgg aatttttcag caattgcggc acgtccagcc
     4261 agtcgaatcg ccaagtgtca tagatcggca aaaaatcttc gagttttccc gtcttgttcc
     4321 aatcgttctt gggccgtccg aacagaggcg cttcggtggt ccagttgatg agctgatttc
     4381 cattgccgtc gatattgtcg atgatcggat agatcacgat cttgccggtt ttgatcgagc
     4441 cgatgcgcaa ataggtatga ccgtccagaa tgggtttgct ccgtgccacg ccgcgccagg
     4501 tgttgatgcc ctcgaacgcc aattcctcac cgggatagaa ttgctttcgc accacggaat
     4561 tgatgccatc gcaggcaatc gcgatatccg cgcgcaccgg cggtagagcc gccccgctgt
     4621 tggtctcctg aaaagccagc gtcacaccgt ccgcatcctg cccgacgccg gtgcaacggc
     4681 gattggtgag gatgttctcc gcgccaagac gttcccgcgc ggcattgaag aggatggtat
     4741 gcagccgtcc gcggtgaatt ccggcctcgg gataagggta gccggcatag cgtccgcgcg
     4801 gctccttata aatgagttgg ccgaaacggt tgaaaaacgc gctttgagta ttcaccacgc
     4861 cggaggccgt cacgagatcg ctcagcccga gcgcggtcag ttcgcgcatc gcgtggggaa
     4921 ggacggtgat gcccacgccc agttccttca cttcgggcgc cgcttcgtag acccgtgaag
     4981 ccagcccgcg tttgtgcagg ccaagagcaa gcgcaaggcc ggcaatcccg ccgccgacga
     5041 tcgcgatgat gttgccggtg cgcgccgtca tcgggatatc agggcgtcgg gattggcgac
     5101 gttgatcggc ttgccgtcga ggaaagccag gatgttatgg atggcgtgct cataaaagac
     5161 gccgagccct tcaaccgtgg tgaagccgag atgcggcgtc agcacggtgt tcggcaaatc
     5221 ccggaacgga ttgtccggcg gcagaggctc gcggtcaaac acatcgaggg ctccgatgat
     5281 ccggccctcg cgcaatgcgg cgagcagggc ggcctcgtcg acgatcggtc cgcgcgaggt
     5341 gttgacgaaa atcgcgcctt tcttcatgcg cgcgaaatca gccgctccga tcagcccgcg
     5401 cgagcgcggt cccaggacca gatggatcgt gaccgcatcg ctctccgcga acagttgttc
     5461 gcggctgacg cgttccgcgc cggcttgctt tgccttttcg tcggtcaggt tctggctcca
     5521 ggcgatgatt ttcatgccga gcgcgtggcc atagcgcgcc acataggcgc cgatgcgtcc
     5581 gagcccgacg atgccgagcg tgcgcccttc gaggttgatt cccggcggga tgccttcctg
     5641 gaagcgcccc gcgcgcatgg ccgcgtcgcc ttgcggaact ttacgtgcgg ccgccaacag
     5701 cagcgccagt gtcatctcgg aggccggcgc aggggagtgt ccgccggagg tgaaggtgca
     5761 gacgatgcca cgcttggtgc aggcgtcctg gtccacattg ggcgcgcgcg aaccggtaag
     5821 cgacatcatc ttcagccgcg gcaggcgtgc gatcaggctt tccgaaaaac gcgaacgctc
     5881 gcgggtgggc agaataatgt cgaaatcctt caattcctgc gcggcccgat cctcgtcggc
     5941 ggccggataa tggcggggaa aaaagaccac ctccgcacgc ttgcgcagtt ctgaccagtc
     6001 cgcgacatcg ggcgcggcat tctgccaatc gtcggtgacc gcgacgcgta tgcctgccat
     6061 tccgctctcc tttttggtgc gcgcatttaa tgctaatgga ggggaaatgc cagcaacagg
     6121 ccaggatttt ttaaggatac cggatgcggc tgcgcgcctg atgttgcaac gaagccgcaa
     6181 ggcggtggcg gaaatatgtt ctagacttgc cagccttgtc ccgacctgac aaaccgcatt
     6241 tgacggaatt aaccacggcg ctcatcctgg aggcatgtcg aagcgtatga atcgtgcgaa
     6301 caatctggcc caagaacaac cccccgtgcc gggagcgttc atcgatctcg atgcgctcgg
     6361 tgccgccgct gtggcacgcg agccgtttgc ctatttcatc gtgccgggct tcgtgaaggc
     6421 cgaggcgcgc gatacgattc gtaccgattt tcctgcgatc cggcatcccg ggagttttcc
     6481 gctcgcgacg ctgcactatg gtcccgcctt tgctgcattc atggatgcca ttcagggaaa
     6541 agcgatggct gcggccgttg gagacaaact cgacatggac cttacggaga gccccaccat
     6601 ggtgacggtg cgtgggcaaa gccgggccgc ggacggcaag atccataccg acagccggac
     6661 caagctggtg acggcgctga tctatatgaa cgattcatgg gaaagcccaa agggacggct
     6721 gcgcctggtg cgctcgcaaa ccgatcttga cgacgtggtc gctgaggtgc cgccggagca
     6781 gggcacgctt ctggtgttcc gcaacgagcc gaatgcctgg cacggtttcg agccgttctc
     6841 cggtccccgc cgcgtcatcc agttgaactg ggtgacggag gcgtccgtgg tgcgccggga
     6901 gctggcgcgg catcgcctga gcgccttctt caagcggctg ggaaaccata aggcgtgacg
     6961 tgcgaatgct gagcccatgc cgctgaacat cgagactttc agcaatgcgg tcggcggcag
     7021 cgccttctac aaagcgctga cgcatccgct gaccgcagat cttgcgcgtg ggctgctcgc
     7081 gaaattgcgg ggaagcgggc cggtggcgat ctacgatccg cataatttcc tggcggcgtt
     7141 cgatgcgatg tttccgctgg agtccattac gattgccggc gtgttcgtcc aggatgtgag
     7201 ccatatcggg cggacgtttc gtgggcactg cgccaaagcc ctgaccgagc tatccgcctg
     7261 ccatgcgcaa gccgtcctga tcgccggttt cgacgcgaaa ccatccatgc agcatctggc
     7321 accattgaca aatggcgccg cgctcctgag cttcgacgcc ttcaggcttc ccgacggcat
     7381 gctgtcggac aagacgcgct atctctccac actcaacttc gccacgaatt tcgtgttttt
     7441 ccgcgatgcc gaagggctgc acacccggct cgcgaccgcg aattattggt ccgcttacgg
     7501 aggaaaaggc ggtcatctgt ggtttgcgct gttcgacgat tcgggggcgc aaatcgcgca
     7561 atgggaagag ccgcttcccg ccgcaaatgc gacaatcgtg gtggacagcc gcgcggtgcg
     7621 ggcgcgcttc aagcttggtc ccttcaccgg ccaattgttc gtccatgtgg taggtgctgc
     7681 aggccacgac atcgtgaaat acgcgctcga tacctatggc gatgaagacg acgtgctctc
     7741 ctgcacccat gacgccaatt cctggccggc caatctttat gccggtttgc cggcgcccgc
     7801 cgacgatgaa gatgtcgtgc tgtgggtcca gaattcccat cccaatccga tcgcgcccgg
     7861 cgagatcggc ctcaacctga tgggcgatga acatatcgct tatctggacg aacccgttgg
     7921 cgctttcgcg acgaaacgtc tgagcgcctc ggcgcttcta cccgtcgcgc gatggccgca
     7981 gcagatcgaa atccacgccg gaaaacattt cgtgcgcccg cgctacgaag tgttgcgcaa
     8041 gaacggccac cggcacatcg cgcaccccaa tgtcgagcgc gaggatttga agctcgacgc
     8101 acagctcccc gatttgacgg cggttctggg caagggattc atcctgcccg ccccggtgct
     8161 gccgatcgag cgtttccgca gcatgatcct gcccacgccg atgtcgaccg tgcagaatca
     8221 tctgccggtg aagctcgtcg tctatgacag cagcggacgc aaggccgcgg agcacgcctt
     8281 cggcaatctt gcccgcaatg attgcgtcgc gctcgccgcc aacgagatcc tcaacggccg
     8341 catcgacggc ggctacggcc atatggaact ggtctatgat ttcgacgcgg gccgcgcggc
     8401 ggatggctgg ctgcacgcgc ttttccgcta cgaagacctt aagaccgggc acgccgcgga
     8461 gaccagtttc ggcgcacata tgttcaatat gcttgtcacc tacaaggatg agccgcaatc
     8521 ctacaaaggc ccgcctcccg gcctgtccac gcggctgttc ctgcgcgtgg gtgccaagcc
     8581 gctggatacg atgtgccatc ttgtctatcc cgcgtcacgc ccctggcatg cggcatcgga
     8641 tacccaattg atgctgatgt cggcgcgcgg cgaagaggta gcgcggcgcg cgctcaagat
     8701 cgcgtgcgga ggttcggcgc tgtggcgcgt cagcgagatg ttcagcgcgg gcgagcgcag
     8761 cgatgccggt gagaattgct acatcgtcgt gcgcgattct acatgccggc tgttcggcta
     8821 tcacggcctc atcggcgggc gcgctttcag cctggatcac atgtttgggt tctgaagctc
     8881 ccccgctttc ctgttgggtc gtgaccgacg gcaaagcggg catggaaagc caatgcgtcg
     8941 g
//
LOCUS       DASYEG010000187         7048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3827847, whole genome shotgun sequence.
ACCESSION   DASYEG010000187 DASYEG010000000
VERSION     DASYEG010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 7048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7048
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3827847"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VGM72_08535"
     CDS             <1..610
                     /locus_tag="VGM72_08535"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEY4265351.1"
                     /translation="NEFQTYSAVLKRAGLKPQQFERYRPWLAAVVVGLAYLHGGNLDS
                     LRGADDDLMDYARAHGRPLAYLESVRDQMSLLVSGEESDHIKALKSLILGLPRSRNQE
                     SELLAGWAAGDAEGFAARLATYFRGHAEAEELLIDARNRNWLPAIEDFLARPAGGTAM
                     ITVGAAHVGGPKGLIALLCHDGYPVERVRERNAGENACGTES"
     gene            614..1366
                     /locus_tag="VGM72_08540"
     CDS             614..1366
                     /locus_tag="VGM72_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265352.1"
                     /translation="MHPARTIRRLGLALSFAPIALTPVLAAGPAATAQTFFAPDSTAP
                     ELANAYLMMHPGDALEISSFGDPANPMQILHAFGPVSRSRAGVCRFTATQVFAHRSDD
                     GTISWDSKPSNPREHAEPPYTMAAVAGNPCPKQNDDAYASLDDGIMDREIIAISNFWK
                     ETSGSESKFDAASVYLSLIVSQRVADAFAAFRTALFQPGGKPPQMQAIFRAGVDGYDL
                     AFGDGRSASSNFFLSISKSGTGFQVLNFQTQF"
     gene            1501..2910
                     /gene="ahcY"
                     /locus_tag="VGM72_08545"
     CDS             1501..2910
                     /gene="ahcY"
                     /locus_tag="VGM72_08545"
                     /inference="COORDINATES: protein motif:HMM:NF017071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY4265353.1"
                     /translation="MTKQDSQDYAVKDIGLAEWGRKEIAIAETEMPGLMATREEFGPG
                     QPLRGARIAGSLHMTIQTAVLIETLTALGAEVRWCSCNIYSTQDHAASAIAAGGTPVF
                     AYKGESLQEYWDYSRRIFEWADGGTPNMILDDGGDATLFVHLGLRAEQGDKGFLRHAA
                     NEEEEVLFALIARTLEEKPGWFAELAKNIRGVTEETTTGVHRLYQMHKEGKLLFPAIN
                     VNDSVTKSKFDNLYGCRESLVDGIRRGTDVMMAGKVAMVAGFGDVGKGSAASLRNAGC
                     RVMISEIDPICALQAAMEGYEVTTMDDAAPRADIFVTATGNLDVITIEHMRAMKDRAI
                     VANIGHFDSEIQVGSLRNYKWHNVKPQVDEIEFPDGKRLLLLSEGRLVNLGNAMGHPS
                     FVMSASFTNQTLAQLEIFCNPGKYEKKVYTLPKHLDEKVARLHLNKIGAKLTEMSDKQ
                     STYIGVAKDGPFKPDHYRY"
     gene            complement(2970..3455)
                     /locus_tag="VGM72_08550"
     CDS             complement(2970..3455)
                     /locus_tag="VGM72_08550"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY4265354.1"
                     /translation="MKRYACLLALALAPFAAGSVSAQMVVEKKVISADAARKIAEGCE
                     AYAKAKGWHVSVWVLDETVTPLYFYRMQGTPMFTVKPALAKAEAAVRTGQPNDAYVRN
                     AETRGPFVATVNAVAGGYVLSPGGLPILIDGQVAGAIGIGGSTDDRACAQAGIDAVFK
                     K"
     gene            3795..6122
                     /locus_tag="VGM72_08555"
     CDS             3795..6122
                     /locus_tag="VGM72_08555"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4265355.1"
                     /translation="MRPAFAADAERLSPDTVQFAIVLAAAALALAGGLWALSEYQSTR
                     HLRQALRIATARARTFLAARDAWLSAEREALLVWGADMAEPLSFGEGAELMQACLGGA
                     QALELSTALDELAANGTPFALTCGTAAGRKIAVRGRPAGGHLTVFFDADSREAPSSLD
                     FRGALNALPLPVWIRGKDQELAFVNRAFLAAAGTTEEDALKSGIVFDRSERDLASTAH
                     SSPETVQAKRFALVAGQRRALSFTLASLPEGGVAGAALDVTDVAEAETRLQRHIDANS
                     DMLHGLATAIVIFGSDQRVNYFNHAFVRLWGLSEVWLESRPRHGDLLDKLRELRRLPE
                     QSDFRAWKQTRTRLFERRETYPEELWHLPTGQTLRVTPRQHPMGGVVVLYDDVSTQLR
                     LESSYNTLIKVQKATLDALHEGVAVFGPDGRLKLYNAAFARIWRMEADELAGEPHLQR
                     ISEACAGRFGRDRSWEIVIASVTSATPERNREWGEIQRSDGTVVALNIAPLPDGATLL
                     SFADVTDRFRIESALRERNEALEASDNLKSEFVKRMSYELRTPLNSILGFAEMLKSGI
                     PGELNARQGEYATAIVSASNVLRNLVNDVLDLSQIESGTIELELEKLDFYGLLFGIAE
                     HAREWATKIGLTFELGCKEDSGQFVGDSRRLKQVVFNLISNALKYTPANGTVTLTGEV
                     HAEDVWISVQDTGPGVPADFLPSAFERFTARGNAVGRPGVGLGLALVNQFVELHGGWV
                     EMESDPDGGTRVTCHLPRDLNTQTLRKPPGIAANG"
     gene            6224..6508
                     /locus_tag="VGM72_08560"
     CDS             6224..6508
                     /locus_tag="VGM72_08560"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEY4265356.1"
                     /translation="MALKKFIIEREIPKVGTLDRAQLREASAKSNGVLRELGTDIQWV
                     QSYVTDNKIFCIYLATDEGKIRTHAEMSGFPASKITEVRKIIDPTTERAD"
     gene            complement(6509..>7048)
                     /locus_tag="VGM72_08565"
     CDS             complement(6509..>7048)
                     /locus_tag="VGM72_08565"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEY4265357.1"
                     /translation="VEIVPNINRIAAAIRQNGGAVVWIQNAATAETLESWSIRVEMDG
                     PERTAARVQSMSPGSKGYALWAGLDTKSEDIFVQKTRFSAFIQGSSNLEALLRARRIE
                     TVVVTGTVTNICCESTARDAMMRNFRTIMVTDANAAMNDEMHNASLAAFYVRFGDIMP
                     ADRLVEILNAGAGIRSAAQ"
BASE COUNT         1401 a         2193 c         2108 g         1346 t
ORIGIN      
        1 aaacgagttc cagacctatt ccgccgtgct caagcgtgcg ggtctgaaac cgcagcaatt
       61 cgaacgctat cgcccatggt tggccgccgt cgtcgtgggc ctggcctatc tgcacggcgg
      121 caatctggat tcgcttcggg gtgccgatga cgatctgatg gattacgccc gcgcgcatgg
      181 acgcccgctt gcctatctcg aaagcgtccg cgatcagatg tcacttctcg tcagcggaga
      241 agagtccgac catatcaagg ccctgaaaag tctgatcctt ggcctgccgc gttcacgcaa
      301 tcaggaaagc gaacttctgg cgggctgggc cgcaggggat gccgagggct ttgccgcgcg
      361 gctcgcgacc tattttcgcg gtcatgccga ggcggaagaa ttgctcatcg atgcgcgcaa
      421 ccgcaactgg ctgccggcga tcgaggattt cctggcgcgc cccgccggcg gcaccgccat
      481 gatcacggtg ggcgcagccc atgtcggggg tcccaagggg ctaatcgcgc tgctgtgcca
      541 cgatggctat ccggtcgagc gtgtccgcga acgcaatgcg ggtgagaatg cctgcggcac
      601 ggagtcataa tccatgcatc ctgcacgaac gatacgccgg ctgggtctcg cgctgtcatt
      661 cgcgccgata gcgctcacgc cggtcttggc tgccggcccc gccgccacgg cgcagacctt
      721 tttcgcaccc gattccaccg cgcccgagct cgcgaacgcc tatctgatga tgcatccggg
      781 cgacgctttg gagatttcct cttttggcga tccggccaat ccgatgcaga ttcttcatgc
      841 cttcggtccg gtctcacgat cgcgcgctgg agtatgccgc ttcaccgcca cccaggtttt
      901 tgcccatcga tcggatgatg ggacgatctc ctgggacagc aagccgtcca atccgcgcga
      961 gcatgccgag ccgccttaca cgatggccgc cgtggcggga aatccgtgtc ccaagcagaa
     1021 cgacgacgcc tatgcctcac tcgatgacgg cattatggat agagagatca tcgcgatctc
     1081 caatttctgg aaggagacct ccggatcgga aagcaaattc gacgcggcgt cggtttactt
     1141 gtccttaatc gtatcgcagc gggtggccga cgcattcgcg gccttccgga cggctctttt
     1201 tcagccgggc ggaaagccac cccagatgca ggccatattc cgcgccggtg tggacggtta
     1261 cgacctggcc tttggcgatg gccgttcggc ttcgtcgaat ttcttcctgt cgatctccaa
     1321 atcgggcacc ggctttcagg tgttgaactt ccaaacccag ttttaaagcg gattttccgg
     1381 gactattcgg gggccgcata tggttatgca taaccatatg catatttctt tatatctttc
     1441 ttgccctccg gccgggacgg ctgatatagg acgggcaccg aaacccagaa gagcgccccc
     1501 atgaccaagc aggatagcca ggattacgcc gtcaaggaca tcgggctcgc cgaatggggc
     1561 cgcaaggaaa tcgccatcgc cgaaacggaa atgccgggcc tgatggcgac ccgcgaagag
     1621 ttcgggccgg gccaaccctt gcggggcgcg cggatcgccg gctcgctgca catgaccatc
     1681 cagacagcgg tcctgatcga aacactgacc gcactgggcg cggaggtccg ctggtgctcg
     1741 tgcaacatct attcgacaca ggaccacgcg gcatcggcca tcgcggcggg cggcaccccc
     1801 gtcttcgcct acaaggggga atcgctccag gaatattggg attattcgcg ccgcatcttc
     1861 gaatgggccg acggcggcac ccccaacatg atcctggacg acggcggcga tgcgacgctc
     1921 ttcgtgcatc tgggccttcg cgccgaacag ggcgacaagg gtttcctgcg ccatgcggcc
     1981 aatgaggaag aagaggtcct gttcgcgctc atcgcccgca cgctggaaga aaagccgggc
     2041 tggttcgcgg agctggcaaa gaacatccgc ggcgtgaccg aggagaccac caccggcgtg
     2101 caccggcttt atcagatgca caaggaaggc aagctgctgt ttccggccat caatgtgaac
     2161 gattccgtca ccaagtcgaa attcgacaat ctctatggct gccgcgaaag tctggtggac
     2221 ggcatccgcc gcggcaccga tgtgatgatg gcaggcaagg tcgcgatggt cgcgggcttc
     2281 ggcgatgtcg gcaaaggctc ggccgcatcc ctgcgcaatg ccggctgccg ggtgatgatc
     2341 tccgaaatcg atcccatctg cgcgctgcag gccgcaatgg aaggctatga agtcaccacc
     2401 atggacgatg ccgcaccgcg cgccgacatc ttcgtcaccg cgaccggcaa tctcgacgtc
     2461 atcaccatcg agcatatgcg tgcgatgaag gatcgcgcca tcgtcgccaa tatcggccat
     2521 ttcgattcgg agattcaggt cgggagcctg cgcaattaca aatggcacaa cgtcaagccg
     2581 caggtggacg agatcgaatt tcccgacggc aagcggttgc ttctgctgtc ggaagggcgc
     2641 ctcgtcaatc tcggcaacgc catgggccat ccctccttcg tgatgagcgc atcctttacc
     2701 aatcagacgc tggcgcagct cgaaatcttc tgcaatcccg gcaaatacga gaagaaagtc
     2761 tacacgctgc ccaagcatct ggatgagaag gtcgcgcgcc tgcatctgaa caagatcggc
     2821 gccaagctga ccgaaatgag cgacaagcaa tcgacctata tcggcgtggc gaaggacggc
     2881 cccttcaagc cagaccatta ccggtattga gcgggaaaaa cgctccacct ctttctgaaa
     2941 ggtgaagcgg ctgagaggtg aagcggcttt tatttcttga acaccgcgtc gatgccggcc
     3001 tgggcgcagg cccggtcgtc agtcgagcca ccgatgccga tggcgcctgc cacctgaccg
     3061 tcgattaaga tcggcaatcc gccgggggac aacacgtagc caccggcgac cgcattgacg
     3121 gtcgccacaa acggcccccg cgtctcggcg ttgcggacat aggcgtcgtt cggctgaccg
     3181 gtgcgcacag cggcttcggc cttggccaac gccggcttca ccgtgaacat gggcgtgccc
     3241 tgcatgcgat agaaatagag cggtgtcacg gtctcgtcca aaacccagac gctcacatgc
     3301 cagcccttgg ctttggcata ggcttcgcag ccctcggcga ttttgcgtgc cgcgtccgcg
     3361 ctgatcacct ttttctcgac aaccatctgc gccgagacgc tgccggcggc gaacggcgcc
     3421 aatgcgagcg ccagaaggca tgcatatcgt ttcatttatt ggatctcccc tgtgacacgc
     3481 ccatcatagc gcagcttcgc gcaaagaccg cgcagaccgg tcaagctgcc gcttgtaccc
     3541 gattgaaaca gaagcctgac ggcagtcttt gcggcttgtg taaactttaa gcctttacac
     3601 ttttttaacc atactctctt gcagcccagg tgcagcctcg tatcatcttg attcttcaat
     3661 gattcgccta aacggcgagc ggggcagcgg gcatgggtcc caaagcgggc caaatacgac
     3721 aaaagcctaa agagaggggg aaacgggctg gaagtctttc gtgggggacg tgtgccggct
     3781 tttttgctgt cacgttgcgt cccgcgttcg ccgctgacgc cgaacgtctt tcacccgaca
     3841 ccgtgcaatt tgccattgtg ttggcagcag ccgccttggc gttggcggga ggcctgtggg
     3901 cgctttccga atatcagagc acacgccatt tacggcaggc cctgcgcatt gcaaccgcgc
     3961 gtgcgcgtac gtttcttgcg gcgcgcgatg cctggctgtc ggcggagcgt gaagcgctgc
     4021 tcgtatgggg cgccgacatg gccgaaccgc tcagcttcgg agaaggcgct gagctgatgc
     4081 aagcctgtct cggcggcgcg caggcactgg agctgtccac cgccctggac gaactcgccg
     4141 ccaatggcac gcccttcgca ctcacatgcg gcaccgccgc cggacgaaaa atcgcagtac
     4201 gcggccgccc ggctggggga catttgacgg tcttcttcga cgcggacagc cgcgaagctc
     4261 cgtcatcgct cgactttcgc ggcgcgctga acgcattgcc gctgccggta tggattcgcg
     4321 gcaaggatca ggagcttgcg ttcgtcaatc gtgcctttct cgccgccgca ggaacgacgg
     4381 aggaggacgc gctcaaatcc ggcattgtct tcgaccggtc ggaacgcgat ttggcttcta
     4441 ctgcgcattc gagccccgaa accgtccaag ccaagcgttt cgctttggtg gccggccagc
     4501 gccgcgcgct cagtttcact ctcgcctcac ttcccgaagg tggcgtcgcg ggcgcggccc
     4561 tggatgtgac ggatgttgca gaagccgaaa cgcgcttgca gcggcacata gacgccaatt
     4621 ccgacatgct gcatgggctt gccaccgcga tcgtcatctt cggctcggat cagagagtca
     4681 attacttcaa ccatgccttt gtccgcctat gggggctttc cgaagtttgg ctggaatccc
     4741 gtccgcgtca tggcgacctc ctcgacaaat tgcgggagct tcgccgcctg cccgaacaat
     4801 ccgattttcg cgcctggaag cagacgcgga cgaggctttt cgaacgccgc gaaacctatc
     4861 ccgaagagct ctggcacctc ccgaccggcc agaccctgcg tgtgaccccc cgccagcatc
     4921 cgatgggcgg cgtggtcgta ctgtatgacg atgtgagcac ccagttgaga ctggaaagct
     4981 cgtacaacac actgatcaag gtgcagaagg cgacgctcga tgcgctgcac gaaggcgtcg
     5041 ccgtgttcgg acccgacggg cggctcaagc tctacaacgc cgcctttgcc cgcatctggc
     5101 gcatggaagc cgatgagctc gccggtgaac cgcatctgca gcgcatttcc gaagcctgcg
     5161 cggggcggtt cggcagagac cgaagctggg agatcgtgat cgcgagcgtg acctcggcga
     5221 ctccggagcg caaccgcgaa tggggcgaaa tccagcgttc ggacggcacc gtcgtcgcgc
     5281 tcaatatcgc gcccttgccc gatggggcga ccctgttgag ctttgcggat gtgaccgatc
     5341 gcttccgcat cgaatcggcc ttgcgtgagc gcaacgaagc gctcgaagcg agcgacaatc
     5401 tgaagtcgga attcgtcaaa cgcatgtcct atgaactgcg caccccgctg aactccatcc
     5461 tgggattcgc cgaaatgctg aaaagcggca ttcccggaga gctgaacgca cgtcagggcg
     5521 aatatgcgac cgcgatcgtg tcggcatcca atgtgctgcg caacctcgtc aacgacgtgc
     5581 tcgatctatc gcagatcgaa tcggggacta tcgaactcga actggaaaaa ctcgatttct
     5641 acggtctcct gttcggcatc gccgaacacg cgcgcgaatg ggcgacgaag atcggcctga
     5701 ccttcgagct cggctgcaaa gaggacagcg gtcagttcgt cggcgacagc cggcgcctga
     5761 aacaggtcgt cttcaatctg atttcaaatg cactcaaata cacgccggcg aatggaacgg
     5821 tgacgctcac cggcgaagtt catgccgaag atgtgtggat ttccgtccag gacaccgggc
     5881 ccggcgtgcc agcggatttt ctgccgtccg ccttcgaacg ttttaccgca agaggaaatg
     5941 ccgtggggcg cccgggcgtt ggactgggcc ttgcgctcgt gaatcagttc gtcgagcttc
     6001 acggcggctg ggtggagatg gaatccgatc ccgacggggg gacgcgcgtg acctgtcacc
     6061 tgccccgcga tctcaacaca cagacgctga gaaaaccacc tggaatcgcg gcgaacggat
     6121 gaaccggctt tacgcggttt agccgctcaa ctagcttggc aacgctccag gtgcagcgcg
     6181 ttccctctgc ggtccacgga gatcacagca tcgggaggat ttcatggcat tgaagaaatt
     6241 catcatagaa cgcgagatac caaaagtcgg cacactcgac cgcgcgcagc tgcgtgaggc
     6301 ttccgccaaa tcgaacggcg tcctgcgcga actcggcacc gatattcaat gggtgcaaag
     6361 ttatgtgacg gacaataaga tcttctgcat ctatctcgcg accgacgaag gcaaaatccg
     6421 cacccatgcc gagatgagcg gctttcccgc ctcgaaaatc acagaggtcc gcaagatcat
     6481 cgatccgacc accgagcgtg ccgattagct attgcgcggc ggaacggatg cccgcgccgg
     6541 cgttcaggat ttcgaccaga cgatccgccg gcatgatgtc accgaagcgc acataaaatg
     6601 ccgcgagcga ggcattgtgc atctcgtcgt tcatggcggc attcgcgtcc gtgaccatga
     6661 tggtacggaa attgcgcatc atggcgtcgc gcgccgtcga ctcgcagcag atgttggtga
     6721 cggtgccggt gacgacgacg gtctcgatgc ggcgggcccg cagcagcgcc tcaagattcg
     6781 acgatccctg gataaaggcg gaaaagcgcg tcttctgcac aaagatatcc tcggacttag
     6841 tgtccaagcc ggcccacagg gcatagcctt tcgatcctgg gctcatggat tggacgcgcg
     6901 ccgccgtacg ctccgggccg tccatctcga cacggatgga ccagctctcc agcgtttccg
     6961 ccgtcgccgc gttctggatc caaaccaccg cgcctccatt ctgccgtatc gcggcggcga
     7021 tccggttgat gttgggcaca atctcgac
//
LOCUS       DASYEG010000188        28990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_639593, whole genome shotgun sequence.
ACCESSION   DASYEG010000188 DASYEG010000000
VERSION     DASYEG010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 28990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28990
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639593"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VGM72_08570"
     CDS             <1..608
                     /locus_tag="VGM72_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265358.1"
                     /translation="FAGIDFNGRYDFDLDRVGLNNFGSLNIGAAGFYEITDKSRTDAA
                     SPIIDNYVGQNSGNRLQRVRYRLGWADDSWSITAFANYFGHGAVNVNGNNLIPPCFYA
                     AGFSPGSCFAGSPYYGPYVTYPNFSPPTVYFDLTVGYQTGEIPANEYLRNIGVQLTVN
                     DIFDKQPPFQVGARGSGSIRAFDNAFTDLQRTFTITVTKVW"
     gene            807..1529
                     /locus_tag="VGM72_08575"
     CDS             807..1529
                     /locus_tag="VGM72_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265359.1"
                     /translation="MNGTAYFRVPGDSGPGPIVDMPGHEYVADYNTRVADTSNPILKP
                     WAKKLMDINNKRVLAGGIPFYPTSRCWPGGVPGLLLYPGEPVFFIQTPKEVWILYQRD
                     SQVRRIYMNVAHSKNPNYSVYGESVGHYESSDTLIVDTIGLDDKGPIDRFRTPHTKQL
                     HVVERYTLKNGGKNIEVAFTVEDSGVFTMPWKAKVDFERGKGARSGGPWEESICADGP
                     RDYFVDDKAGAVPIPQAERPDF"
     gene            1596..1952
                     /locus_tag="VGM72_08580"
     CDS             1596..1952
                     /locus_tag="VGM72_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265360.1"
                     /translation="MSVKATLRSTGMLAVTIVLLLGGGYAFAQSGGLNPRLSGDIVPA
                     PNGYEAVAVKYIVTRSTDLYISPFIWAGKVLGVHLNAGKSVDALAKLNGYDWLLVGKN
                     GTGIGYVPISALSPVK"
     gene            1949..2383
                     /locus_tag="VGM72_08585"
     CDS             1949..2383
                     /locus_tag="VGM72_08585"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HEY4265361.1"
                     /translation="MTLSALRGETMNWKTLGLLSAMAAVVAVMPAVAHHSYSMFALDK
                     SVTIKGTVKEFRWTNPHAWILMMVPDAQGKSEQWSIQMGSPGSLARRGWNSKTIAAGD
                     NVSMTLHPVKDGTHGGEYTSITLPSGKVLNDLMGGQHTGEND"
     gene            2403..3149
                     /locus_tag="VGM72_08590"
     CDS             2403..3149
                     /locus_tag="VGM72_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265362.1"
                     /translation="MSERSRMPPWLFVVWSVVLSVAFPAAAAQDQALGQDINGIYWVS
                     NYSAKIQPIDGGNPPYTPAATAIYEKNIAAAKANTLEDNARKFCVPDGVPRFLESPYP
                     FEIVQTPTRKEIHILYELNHGVRLIQMDRPVPSEQELLELPYYSGHSVGHWEDGTLVI
                     QTVGFNDKTFLDNSGAPHTDQLRTVERIRKINGGKQLEDVITIHDPGVFTKDWSARFV
                     YEKRTDIRIMDYTCGEKHRDLSSVKGVRIP"
     gene            3348..4064
                     /locus_tag="VGM72_08595"
     CDS             3348..4064
                     /locus_tag="VGM72_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265363.1"
                     /translation="MLTKGTQYLKVPDDSGPGPVVQKGKPYKHDVVPRIADTSNPILK
                     PWVKKLMDINNERVIAGGLPFVTASRCWPGGVPGILIYPGEPLIFLQSPDEVWIIGRD
                     EQVRRIYMNAPHSQKPSYSVYGESVGHYENGDTLVVDTIGLDDSGPIDRYRTPHTKQL
                     HVIERYTLINGGKDLKVVFNVEDPGTFTMPWKAEVDFARGKGNSGTWKENVCSENTED
                     FFLDGRNDVVPEPHADKRNF"
     gene            4300..5367
                     /locus_tag="VGM72_08600"
     CDS             4300..5367
                     /locus_tag="VGM72_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265364.1"
                     /translation="MKKSSAFICAAALVLTGFASHAATGYHLIKSIPISGDWTWDYIG
                     MDTAHRHVFVSHGPELEILNADTYQIDGKIVAPGVDFSKPETLAVMNVRGAAAAEDIG
                     RGFVPNARDGSVSIFDLNTLKVLATVKVGENPDGYLYDPATHRGFTFSNRSKGAIAVD
                     LRDAKLAGTVPLGSKPEGGAADGKGHIFVDMQEKDVVAKIDSRKLMLEQSWPTAPCKQ
                     PTSMAIDTKNDRLFIGCRGTSPMLVVMDSTNGKIVTTLPIGNGTDAAAFDAEKRLIFT
                     SNGGDGTITVVQEESPDKFAVVDTVKTQPGARTMTLDTRSHKLFLITANRTTPPAVAG
                     QPAPRPQMVAGSFRVLVVGAE"
     gene            complement(5431..5587)
                     /locus_tag="VGM72_08605"
                     /pseudo
     CDS             complement(5431..5587)
                     /locus_tag="VGM72_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611992.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            5798..7180
                     /locus_tag="VGM72_08610"
     CDS             5798..7180
                     /locus_tag="VGM72_08610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family XrtA/PEP-CTERM system
                     glycosyltransferase"
                     /protein_id="HEY4265365.1"
                     /translation="MNASTALFLIDALLVSVAWPLSVWLATNGAAPQEAWFQALIFAT
                     VNQTFLYALGLYRREALVDIGKTLGRIPLVVAIGVVTASFATAAFGWTLSPIPYTASN
                     LAALFIAAVACFTVCAMLARLAFLMLRRHRLFRPQLLVIGAGKRAWDLTWMLRSQGRN
                     LHYAVTFVHDDVFGAVDPRLARDPANRIVPASENLLRIADRVRADQIVVAPDERRGMD
                     LEELIACRTAGYPVSQYMSFLEQEVGRIDIKRLDLTWVLYSVGFDFGPVDRALKRMLD
                     IAVSLLILSVFFPVLLLAMLAVWLEDRGTMLYRQERLTKAGRRFQILKLRTMRMNAES
                     QGAVWAAASDKRITRIGAFLRRSRLDELPQLFNVLIGDMALVGPRPERPEFIAELSKQ
                     LPLYQERHAVKAGLTGWAQINYPYGASLDDARSKLSYDLYYVKNFSIFFDLQIIMQTL
                     RVVIWPSGVR"
     gene            complement(7206..8036)
                     /locus_tag="VGM72_08615"
     CDS             complement(7206..8036)
                     /locus_tag="VGM72_08615"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4265366.1"
                     /translation="MRCSALLIAAMPVFCAPSAQAAARTPNIDTGPSSSQMLQCNSLD
                     PSRAIIGCTAIIQSSGPADLKSAAYSTRGLAYRKRAENDRAIADLDQAIALVPKSANA
                     FRSRGDFYQFQGDYERALADYNQTIAIDPKDGEAFNQRGNTYLLKDDPEHAFPDYDKA
                     IAINPKSVVAYSNRGEAYRVQGDDDRAIQDFDSALRLDAKFVAALVSRGLAEADKGDF
                     PRAIEDFTKAIALDPKFIAAYDQRANAYEQTGAHDRAIADIAQAIKLDPENPGSHRLP
                     "
     gene            8353..9411
                     /locus_tag="VGM72_08620"
     CDS             8353..9411
                     /locus_tag="VGM72_08620"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HEY4265367.1"
                     /translation="MTNFRRMLKISCFALALMPFAASAETALVYVTNSAGDHIDVINP
                     LTNKVVQKFPIIGAHGVNFSPDGSRVYVSNESTSTLDVFEQKTGKLMKKIKLSNHPNN
                     IAVAKDGRIVVGIARGQGALDIVDPVKLEVIKTIPTHGRLHNCYTSKDSAYAICGSVQ
                     TGMFTAFDLKTQTIAWEHKFDHGVRPMTIESNADGSPKRVFVQLAAFDGFAILDFKTH
                     EEVARVKLPDLKTGVDMDPGREDAPSHGIGVSPDGTTLWVTSIPQNAVFTYNLATPIT
                     AESKPIDTIRLPDLQIPGKRLGGAVANWVTFTPDGQVYIANSGLRSVTAINMATKKIS
                     AVVPVGEVPKRINTLVMR"
     gene            complement(9490..9924)
                     /locus_tag="VGM72_08625"
     CDS             complement(9490..9924)
                     /locus_tag="VGM72_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265368.1"
                     /translation="MSARLTLLFAVAATLGIGAIAVGVSQAAPAPGLDYEFFKTKVEP
                     IFLKERPPHEPCYNCHEGANNFLHLEPLAEGQKFWTEEQSRKNFESISRLVTPANKAE
                     SRLLIHPLAPEAGGDLFHSGGRQFESKNDPDYKILMQWVGVQ"
     gene            10071..10541
                     /locus_tag="VGM72_08630"
     CDS             10071..10541
                     /locus_tag="VGM72_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY4265369.1"
                     /translation="MPQTYHLRVNGESREVSVDGDTPLLYVLKNDLELPGAKFGCGLA
                     QCGACTVIMDGAAVRSCVTEVRSVGTRDIVTLEGIGTEQKPHPLQAAFIAEQAMQCGY
                     CINGMIMSAKALLDRNPHPADGEIREALAGNLCRCGSHNRIVRAILRASKEAAT"
     gene            10538..12763
                     /locus_tag="VGM72_08635"
     CDS             10538..12763
                     /locus_tag="VGM72_08635"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY4265370.1"
                     /translation="MNKVLTRREFGTAFGGLVIAFSMGPRLAAAADGPPRLPGTLAAN
                     KRLDSWLRVDPVGTVTVFTGRVELGQGATTALAQIAAEELDIAFVRIRMIPVDTSHSP
                     NEGVTAGSNSIEMGGAALRSAAAEARAILVQLASEHLNVPAAQLMTEDGLIFARDGAR
                     KTSYWELAAENPLDREATNSAPAKPVKDHRIVGQPIQRLDIPDKVMGRPVFIQDMRLP
                     GMVFGRVVRPPNYGAELTRLDDAAVRKMPGIVSVVRDGRFVGVVAEREEQAIAARAAL
                     AKAAVWRVPASLPDAANVHEFLKTRPGVVPKIVNEKTGMAAPAMKTLEAVYRKPYLAH
                     ASIGPSCGIAGFIDEGLTVWSHTQGPYPLRTDIAKVTAMPPEKVRVIHTPGAGCYGHN
                     GADDAALDAALLSLATNGRLVKVQWMRDDEFGWEPFGSAMTMSARAGLAADGSIVDWQ
                     YDVWSCTHNMRPGNRSGVNLLGAWHLAKPLARATPAEAPLPAGGGDRNAVPVYDFPNQ
                     RITDHFIADMPLRVSALRTLGAFGNVFALESFMDEMADAAGADPVAFRLKHLKDARGR
                     AVLQAVAEKANWKPGTKGDGARGRGVAFSRYETTKTYVAMIADVVVDRTSGMVRVERV
                     TAVADAGQTVNPDGLKNQIEGGIIQGTSWTLKEQVVFDKERIVSRDWVGYPILTFPEV
                     PVVDVVVIDRPELPSLGAGEASQGPISAAIANAIRHATGARLRDLPFTPERARAAIAQ
                     I"
     gene            complement(12854..13558)
                     /locus_tag="VGM72_08640"
     CDS             complement(12854..13558)
                     /locus_tag="VGM72_08640"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEY4265371.1"
                     /translation="MALLVQLQQQSPTDGTQASAAQPAANDPVQKLFSAMDGNGDGTV
                     SQKEMETYIGSQGGTQAQADALFSSLTQNAGAQDASGQNASAGISEQQLGADVSQTQH
                     AGHHHHHHGGGAGSSQDANSSSDIATQILGALDTDQDGTISSDELATAFNTNSSASGT
                     GTSSTDSSTLFSQIDSNGDGSVTGDELGSFLSSLAQQTQSNFNTGGMFGQLAAQSYDA
                     SAGLLNKTGSGQSSYA"
     gene            13602..14021
                     /locus_tag="VGM72_08645"
     CDS             13602..14021
                     /locus_tag="VGM72_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265372.1"
                     /translation="MLAVLSVAAELESGAAVLELEPVLDCVLLVLPSRESILARSWPA
                     SAAPDAPTPFMDMEISPLTRLRTPAAIADGAEGQSIFHARGPHGRQGMKCMVYERLRG
                     KNYPARLRASPFMGGRFVAKNGPKLYRAIMSESFCGL"
     gene            complement(14048..14878)
                     /locus_tag="VGM72_08650"
     CDS             complement(14048..14878)
                     /locus_tag="VGM72_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265373.1"
                     /translation="MRIAASRGWRSGIALLALVWTCDAAQGATSATPDFSGFWQHGVP
                     GQQYDKPPSGPGPVRRIGVPENYAANLNWHGDDSNPILQPWAADAVRKEWARESQGLP
                     ELSAQTTCRPAGVPFILSFLRPMQILQTPKKVVFLYQFDHQSRVVYLDQPHTPKLAPS
                     YYGESVGHYEDGALIVDTIGLNDKTWTDRFATPHTEQLHVIERYRLIDEGKTLEVFFT
                     VEDPGAFTTKWSAITRYPRVNVARIEEEACAENNAGYGITVPVADFDPISGQALHDPR
                     "
     gene            15002..15187
                     /locus_tag="VGM72_08655"
     CDS             15002..15187
                     /locus_tag="VGM72_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265374.1"
                     /translation="MATRSKNAKTDKPRTQDEKLDDALDATFPASDPPSMTEPTKHVG
                     SGKKTGAPTPVGDPNLK"
     gene            15267..15860
                     /locus_tag="VGM72_08660"
     CDS             15267..15860
                     /locus_tag="VGM72_08660"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY4265375.1"
                     /translation="MAQTIFRSANRETSALAATIARNIRRLRFRRGLTVEKLAQLAEI
                     NLLTVDQMESGKGELTLALAWKLANALDVPFAALMAEHAPGGTVITRKDRAKVIVSEN
                     LGVASRALFPFDEDARVEFYELRIAPDHRETSQPHAVGTREMLHVAEGAFEVSVGREP
                     PYRVNKGDTIHFLADLPHSYRNLTAMPATAYLVMTYQ"
     gene            15935..16594
                     /locus_tag="VGM72_08665"
     CDS             15935..16594
                     /locus_tag="VGM72_08665"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY4265376.1"
                     /translation="MALRLGDIAPDFQADTTQGPIRFHEWLGDSWGVLFSHPKDFTPI
                     CTTELGYMARIKPEFDKRNVKIIGLSVDPVDRHSGWAKDIEETQGTAPNYPIIGDPDL
                     AISKLYDMLPAEASGDPSKRTAADNQTVRNVYVIGPDKKLKLILIYPMTTGRNFDEVL
                     RVIDSIQLTARHKVATPVNWKPGEDVIISGSVSDEDAKKTYPQGWKAPKPYIRIVPQP
                     R"
     gene            complement(16600..17847)
                     /locus_tag="VGM72_08670"
     CDS             complement(16600..17847)
                     /locus_tag="VGM72_08670"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4265377.1"
                     /translation="MSTPNPLFTAEEFKPRSWDLRLYLAIRFGTTLGTQIQSVAIGWQ
                     VYDLTRDPVALGYVGLSIFLPMLLLVLPAGDLADRVDRRRMLMASYLVQVVASVMLLG
                     LTLTGVKAMWAFYSVITLLGVALGLSQPAMQSFLPFLVTLDKLPQAIAWNASAYRIAV
                     IGGPAVGGFLYDLGPVVNYSLCLCLYIFTLVGMAILRIRPQQSSAGQGNALQRIVDGI
                     SYMRARPILLGAISLDLFAMFLGGTTALLPIFARDILHTGPAGLGFLRTAPAMGAALV
                     AFVLARWQLHRHTGISMFGCVAIFGIATIVFGLSRDFYVSMVALAVSGAADMVSVYVR
                     SALIQLATPDNMRGRVGSLNSLFIGASNELGEFRAGMTAGALGTVPAVVLGGIGTVLV
                     VGLWMALFPPLRRVDRFSDVAVG"
     gene            18126..19472
                     /locus_tag="VGM72_08675"
     CDS             18126..19472
                     /locus_tag="VGM72_08675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY4265378.1"
                     /translation="MMGYDEPSSKRHATVYGTHPAVKQRNPWPRRILLAVIALGVGGY
                     IAANPDVVQKISSLTTSAPAGGRGAARNAGAVPPVRVAMAETHDVPVVVHTIGTVLAN
                     SIVTVKSQVDGPLLSAMFREGQMVKKGDLLFRIDPAPFEAALRQSAAMVERDQAQYDS
                     AKADADRAVMLADRGIVAAQQRDQLVATAKALAATVAADKAAVERAQLNLDYTTIRSP
                     IDGKTGAFIIYPGNQVHASDAAGLVTITQIQPVKISFFLPQGDLPQLQDRMRENQLVV
                     DITTRSDVAAEPAAAPAAATAGASDGDIPVKVDFIGNTVDDKTGTIELRATFQNPDLR
                     LVPGQLVDVSANLETFKNVVTVPRDAVNVGQDGNYVYVIDKDMKAQMRPVKVLYQDQV
                     MAALGSGIANGERVVTDGQLRVTPGTTVAITHPEGETPAKAAPQANTDASGTGRGG"
     gene            19500..22646
                     /locus_tag="VGM72_08680"
     CDS             19500..22646
                     /locus_tag="VGM72_08680"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY4265379.1"
                     /translation="MHISAAFIKRPVMATLLMAGFVLAGLFGYSSLPVSELPNVDFPT
                     IDVNASLPGADAQTMASAVATPLEKQFSLIAGLDSMTSQNAQGQTRITMQFRLDRNID
                     SAAQDVQSALSAAARQLPQAMPTPPSIRKVNPSEGSILFLTVSSATLPLSEVNSYVEN
                     LLIGKLSSINGVAQADIFGQARPAVRVQVDPNALALRGIGIDEVATAISRSSVNMATG
                     QLDGTTRGAVIHADGQLNNAEQYASQIIAYRNGAPVRFADVANVIDGVENPRNYGGWG
                     TKEGTIPAVTVGIDRQPGANTVAVVDAIRAALPQLVAQLPPSIKVDVTLDRSNSVRQA
                     VSDVQLTLLIAAFLVIGVIFVFLRTVSATFIPAIALPIAIIGTFAGMAVMGYSLDNLS
                     LMALTLCVGFVVDDAIVMLENIMRHVEAGEEPYQAAMIGSKEVTFTILSMTVSLIAVF
                     IPVIFMGGIVGRLLHEFSITIAIAVIVSGIVSVTLTPMLCSRLIKSAKEEHSKHHNVF
                     YRVSERGFLAMQRGYERSLRWTMSHRPLIFVIFLLSILATVQMFRIMPQDFLTAEDTG
                     QINAFTDGANGVSFAEMRRHQDEAARILAADPNIDGFMTSVGSGGTRGGLNSGGFRIQ
                     LKPRDQRPLGVNDVMAELRQKFQRIPGINVVMQNRPPINIGGYVSRALYQYNLQDIDL
                     KELYTWSQRLQEALAKNPNFVDVNSDLDLSTPSVEVSIDRDKAAALGITTQQIETALG
                     AAFGGQRVAPIYTQADQYWVILELLPQYQQDADALSRLYLATPRTASVSTSNVGASVP
                     LSAVTTIRRGTQPLSINHSGQLPAVTLSFNLPQGVALSNAVAALEQIKRDIGFPASVQ
                     GNFQGTAKAFQDSQKGMGFLLIGGILVVYIVLGILYESFIHPLTILTGLPSAAVGALI
                     TLWLFHYSLTLYAYVGMIMLVGLVKKNAIMMVDFALTRQRGEGIEPEAAIVDAAVVRF
                     RPIMMTTMSALMGTFPIAVGWGSSSEGRKPLGLAVVGGLIVSQAVTLYITPVLYVYLD
                     KLGSALGRKRKPIAIPAE"
     gene            complement(22651..23766)
                     /locus_tag="VGM72_08685"
     CDS             complement(22651..23766)
                     /locus_tag="VGM72_08685"
                     /inference="COORDINATES: protein motif:HMM:NF019077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1513 domain-containing protein"
                     /protein_id="HEY4265380.1"
                     /translation="MAIDWTIHRRGLFKGAAAALLGGTFASKTRASAADRPAVSLVSA
                     ARIGETDGGIRIDAQGVSGFSLPGRAHALTALSGGEVLLVGRRPGEFAAIVNLEMPQA
                     EPRIFHPAAGHRFAGHAALHPDERMLVTSEIDAETGAGAVVVRDAQDGRPRAIFPVGI
                     EPHDLLFARGGARLMVAIGGIAKAADVKGPPINAGNIDSAILELDPETGAILKRHTLG
                     PSLKSLSLRHMALAPDGETLAFGMQDQDRAQLRPLMGILRIGADIALLPLPGDDEGSL
                     RSYVGSVAIDSSGRFAAAASPKGGRVDLWSLGDERWLGGYTAADVCGLAADAQAACFW
                     ATSGLGDVMKLRASDAGLQADAHWRAPAAFDNHLLRI"
     gene            complement(23756..24859)
                     /locus_tag="VGM72_08690"
     CDS             complement(23756..24859)
                     /locus_tag="VGM72_08690"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HEY4265381.1"
                     /translation="MVNVSRNSLLVLAAAPVPAKAAAQSAPAKPDPKAVQAGKIALAV
                     TDRFVIPTYRALVQASAAQEAAWVAFAAKREAGDFTSLRAAYNADADAWAKAQLIKTG
                     PITLFLRYDRFAYWPEARNATQRALDQLLMSKDPKDLTADSLAHDSVAGQGLTALERL
                     LYDDDAAKLLKAPGKDGVWRTQVGVAIAQNLSAIAREVLADWTAPDGVRAAIAANKGW
                     KNLFADAPEAARLLLTDLVAAFKLMHDVKLLPVLGANAAAAKPKSAEAWRSGRTQRDL
                     ALNLAAAQEWETAFAATAPAAHRAKLGSLFAAAVKAVDAMPADAGAAAADPKRRARVD
                     AGRAAIKSVQTEIAKTLPGDVGVTLGFNSLDGD"
     gene            complement(24869..26062)
                     /locus_tag="VGM72_08695"
     CDS             complement(24869..26062)
                     /locus_tag="VGM72_08695"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEY4265382.1"
                     /translation="MVPGRLIAALGLAAAAPLLTGASLDLAIGKHLFERNWVSAPSST
                     KSDDGLGPLYDAMSCAACHTSNPRDRASESALPAGMVIRLGNGRGEGDPVYGVQLQTH
                     GVAGQVPEAAPAIQWTGKGDLRIPTIVPGSLGYGPLAKDTRLSLRRAPSLKGAGLLAQ
                     IPESEILSRADSEDADHDGIAGRVPWVTIDGKRVLGRFGWKATQPDLPAQVAAAFSRD
                     IGLSTRLRPEPWGDCTAAERACRAGPHGSEDGAPEVADPLFDLIVGYVAALSPPQPAP
                     AKFAAGKTLFVSIGCAVCHATLHLANGKPVPAYTDLLLHDMGGELGDGIEEGSADSWF
                     WRTAPLWDVAGSLKTGGLLHDGRAGDVTEAIEWHGGEARGTRDRFRALTQSDKAALVA
                     FVSGL"
     gene            complement(26044..27510)
                     /locus_tag="VGM72_08700"
     CDS             complement(26044..27510)
                     /locus_tag="VGM72_08700"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEY4265383.1"
                     /translation="MPGTRPALTRRALMAQGGVLAAVTLASCGRFARSARTVPVGRLP
                     EYRIGGVPTSLVAQERPVMLGASTGLTPAWLYGNAPFPVYRLHLGQSVEATLTNRLKE
                     HSSVHWHGVRGPNAMDGVAYVTQMPVEPGENFVYRFTPPDPGTYFFHPHCNTAEQLGR
                     GLAGVLIVEDGVAFDDDVVCVMKDWRVAKDGQFLAFITPQGAARGGTFGTLRTVNGQV
                     APEITVPAGANIRLRVLNLDSTRISDIGVQGARACVIAVDGNALTPFALESWRMGPAM
                     RMDVALRTPDAGGRIALIDYFAAQPVTLATLVAQGASKASSATGPALAPARLPEPDLD
                     KAETHELKLSASATPTDYAGLAPIVLPDGRKIDVLDSLCSTAQTLWAIDGKTWPQNGH
                     EHLPPPLMSFSRGTPVRLQFNNTTPHMHPMHLHGHTFKVLSASKMKRPVHWADTVLVL
                     PEERVEIAFVADNPGNWMIHCHIIEHQDTGMMGWFRVV"
     gene            complement(27518..28789)
                     /locus_tag="VGM72_08705"
     CDS             complement(27518..28789)
                     /locus_tag="VGM72_08705"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HEY4265384.1"
                     /translation="MRRLFAFSVAGALLSGAALAASAVQPKPVDVLNTYGDLAHAMYE
                     DSFITAKALQGAVNAFLADPTPAKLRTARDAWKAARVPYLQTEAFRFGNAIVDEWEGE
                     VNSWPLDEGLIDYVAASYGKTSDENPLYTLNVIANRQIRIGAKQVNASVIDRNLLRQL
                     QQAQGVEANVATGYHAIEFLLWGQDLHGTGPGAGERPASDYSQKNCTHANCDRRAQYL
                     KAATDLLVDDMAEMTADWAAGGKARKDLAAKGQNGGLSTVLTGIGSLSYGEMAGERMK
                     LGLILHDPEEEQDCFSDNTHNSHYYDELGINEIWRGRYTRVNGTMVQGASFRDYVVAK
                     NPAVAKRMDDRIADTLAKIKSIKDMGDSGKMAYDQMIAKDNAAGNRLVQDAIDALIAQ
                     TRADEAVVAALGLKISLEKSDSLDNPSAVTR"
BASE COUNT         5709 a         8913 c         8815 g         5553 t
ORIGIN      
        1 atttcgcggg aatcgatttc aacggtcgct acgattttga tttggacaga gtgggtttga
       61 ataatttcgg atcgttaaat attggtgcgg ccggatttta cgaaattacg gataagagcc
      121 gaaccgatgc ggcatcaccc atcatcgaca actacgtcgg acagaattca ggaaaccggc
      181 ttcagcgtgt tcgctatcgg cttggttggg ccgacgacag ttggagcatt accgccttcg
      241 caaactattt cggccatggt gccgtcaacg tcaacggcaa caacctgatc ccgccgtgct
      301 tctacgccgc cggattctcg cctggctcct gctttgcagg ctcgccctat tacggaccat
      361 atgtgacgta tcccaacttc tcgcctccca cggtctattt cgacttaacg gtcggatatc
      421 aaaccggaga aattcccgcg aacgaatacc ttcgcaatat aggcgttcag ctcaccgtga
      481 acgacatttt cgacaaacag ccgccgttcc aggtcggcgc gcgcggtagc gggtccattc
      541 gcgccttcga taatgccttc acagacttgc aacggacgtt taccatcacg gtgacgaaag
      601 tctggtgata cgtggccgca aaccattgtg aacatggcga gccgatttcg ctgaagacct
      661 actgcttatt gcggggccgg ctcgtcggaa tagcagcatc ccttctctgc accgcagcgg
      721 tttggtccgg cttgcacgcg cagcctgtgg cggggccgga tggtacggta cccgacttct
      781 cgtccaacaa gatgacttgg gttctgatga acggcaccgc ttatttcagg gtgccgggcg
      841 actccggccc gggtccgatc gtagacatgc ccggccacga atatgtcgct gactacaaca
      901 cgcgagtggc ggatacgagc aacccaattc tgaagccgtg ggccaaaaag cttatggaca
      961 taaacaacaa acgggtactc gccggtggca ttccattcta tcccacgtcc cgttgctggc
     1021 ctggcggcgt ccccggtctt ttactctacc cgggagagcc cgtatttttt attcagactc
     1081 caaaggaggt ctggattctc taccagcgcg attcccaggt gcggcggatt tatatgaacg
     1141 tagcgcacag caagaatccg aattattcgg tgtacggcga atcggttggc cattacgaga
     1201 gtagcgatac gctgatcgtc gatacaattg gattggacga caagggcccg attgacaggt
     1261 tccggactcc acataccaag caattgcatg tcgttgagcg ctatacattg aaaaatggcg
     1321 gcaaaaacat cgaggttgcg ttcacggtcg aagactccgg cgtattcact atgccttgga
     1381 aagccaaggt ggatttcgag cgtggaaaag gcgcgcggtc gggtgggcct tgggaagaat
     1441 ccatttgtgc cgacggtccg cgggattatt ttgtcgacga taaggccggc gcagttccga
     1501 taccgcaggc agagagacct gatttctaga cgaaataaat gggtcggtga acgatgagaa
     1561 cgaagcgtct atcatggagt ttatgggagc ggccggtgtc tgtgaaagcc acgctccgaa
     1621 gcactggtat gttggcggtg accattgtgc tgttgttggg cggtggatat gcgttcgctc
     1681 agtcaggtgg acttaatccg cgtctgtcgg gtgacatcgt tccggcgccg aatggctacg
     1741 aggcagtggc cgtcaaatac atcgtcacgc gttcgactga cctttacatt agcccattca
     1801 tctgggctgg gaaggtcttg ggtgttcacc ttaatgctgg taagtccgtc gacgcactcg
     1861 caaagctcaa cggttacgac tggcttctcg ttggcaagaa cggcaccgga atcggctacg
     1921 tcccaatttc ggctctgtcg ccagtgaaat gacgctttca gcactccgag gagagacaat
     1981 gaactggaaa acccttggcc ttttgagcgc catggcagca gtggtcgctg tgatgcccgc
     2041 cgtcgctcat cattcctatt ccatgttcgc cttggacaag agcgtcacaa tcaaaggaac
     2101 cgtcaaggag ttccgatgga ccaatccgca cgcctggatt ttaatgatgg tgccggatgc
     2161 gcaaggcaaa tccgaacaat ggtccattca gatgggaagt ccaggcagcc ttgctcgccg
     2221 gggttggaat tccaagacga ttgcagcggg cgacaatgtt tcgatgacgc tgcatccggt
     2281 caaggacggc acccatggcg gggaatacac gtcgattact ttgcccagcg gcaaggtgct
     2341 caacgaccta atgggaggtc agcataccgg tgaaaatgac tgagttctac gcgggagctg
     2401 taatgtctga gcgaagccgt atgccaccat ggctttttgt ggtctggtcc gtggtcctgt
     2461 cggtggcatt cccggccgcc gctgcccaag accaagcctt ggggcaggat atcaacggga
     2521 tttattgggt tagcaactat agcgccaaga tacagccgat cgacggcggc aacccgccgt
     2581 acacgcccgc ggcaacggcg atctatgaaa agaacatagc ggcggccaag gcgaacacgc
     2641 tcgaagacaa cgcccgcaaa ttctgcgtgc cggacggcgt gccgcgcttt ctcgaaagcc
     2701 cttacccctt cgagattgtg cagactccca ctcgcaaaga gattcatatt ctctatgagc
     2761 tcaatcacgg tgtccggcta atccagatgg acagaccggt accatcggag caggaactgc
     2821 tggagctgcc ctattatagc ggccactcgg tcggtcattg ggaagacggc actttggtca
     2881 tccaaaccgt cgggttcaac gataagacat tcctcgacaa ttccggtgct ccgcatacgg
     2941 atcaattgcg cacggtcgaa cggatacgca aaatcaacgg cggcaagcaa ctggaggatg
     3001 tcataaccat ccacgatcct ggtgtgttta ccaaggattg gagtgcgcgg tttgtctatg
     3061 aaaagcggac ggacatccgc attatggatt acacctgtgg agaaaaacac cgagatctat
     3121 catcggtgaa gggcgtccga attccttagt tgccggatgg cacagacgtg acggggagaa
     3181 aaacgatgcc cataagccta agtctcgcgt cgcgctgctt atttcctcga ggtattgtgg
     3241 cgggacttct tctttgcctc gcatcggttt ggccggtctc gggtgcgcga ccagccgaca
     3301 agacgagcaa cgccataccg gatttctcgt caggcgatag aacatgggtg ctgacgaagg
     3361 gcacgcaata cttgaaggtg ccggacgatt caggtccagg ccccgtcgtg caaaagggca
     3421 agccctataa gcatgacgtc gttcccagga tcgcggacac cagcaaccca attctgaagc
     3481 cttgggtcaa gaagttgatg gatattaaca atgagcgagt gatcgccggc ggccttccat
     3541 tcgtgactgc ttcgagatgc tggcccggag gcgttcccgg tatcctcatc tacccgggtg
     3601 agccgttaat tttcctgcaa agtccggatg aggtctggat catcgggcgc gatgagcagg
     3661 tgagacggat ctatatgaac gcaccgcaca gtcaaaagcc cagttattcg gtctatggag
     3721 aatcggtggg gcactatgag aacggcgaca cgctggttgt cgacacgatc ggattggatg
     3781 atagcggtcc gattgaccgg taccgaacgc cgcacaccaa gcaattgcac gtgatcgagc
     3841 gctacacact gatcaacggt ggcaaggatc tcaaagtggt tttcaacgtc gaggacccgg
     3901 gcacgttcac gatgccctgg aaggcggaag tggactttgc gcgcggcaaa ggaaatagcg
     3961 gcacttggaa ggaaaacgtc tgcagcgaaa ataccgaaga ttttttcctg gacggtagaa
     4021 acgatgttgt tccggagccg catgcggaca aacgtaattt ctgagtggaa tacgcgcggc
     4081 gaggccggcc ttccggcgga caagtccgcg catgtccgct cgccacagac aggctgagaa
     4141 tgatgattta acccttggcc gaatggtgcc tattcgcgat ctcgacgggg tgtgccggtg
     4201 atgtgcccag atcactgact tgtccgccga gagtttagtc ggcaccagct tcgctgcggg
     4261 catttgcagg atcgaccatt ccttcgtcag gagcatccca tgaagaaatc ttctgccttc
     4321 atttgtgctg ccgcactcgt cctgaccggg ttcgcatctc acgccgctac tgggtatcat
     4381 ttgattaaat cgatccccat ctcgggagat tggacttggg actatatcgg tatggatacc
     4441 gcccaccggc acgtattcgt ctcacacgga cctgagttgg aaattctaaa tgcggacacc
     4501 tatcagattg acggcaaaat cgtcgcgccc ggagtcgact tttctaagcc cgaaaccctc
     4561 gccgtcatga atgtgcgcgg cgcagcagcc gccgaagaca ttggccgcgg cttcgtcccc
     4621 aatgcccggg atggctcggt ttccatattc gacctgaata cgctgaaggt cctcgccacc
     4681 gttaaagtgg gtgagaaccc cgacggctac ctctacgacc cggcgactca ccgcggcttt
     4741 accttcagca acagaagcaa gggcgccatc gccgtggacc tgcgcgacgc caaacttgcc
     4801 ggcacggtcc cgcttggcag caagcctgaa ggcggcgcgg ccgatggcaa gggacatatt
     4861 ttcgtagaca tgcaggaaaa ggacgtcgtc gcgaaaatcg attcgcgaaa actgatgctc
     4921 gaacaaagct ggccgacagc gccttgcaag cagccgacca gtatggccat tgacactaag
     4981 aacgaccgcc tgttcatcgg ttgtcgcggc acctctccaa tgctcgtggt gatggattca
     5041 accaacggca aaattgtcac taccctaccg atcgggaacg gcaccgatgc ggctgccttc
     5101 gacgccgaga agcgattgat ctttacctcc aacggaggag acggcaccat cacggtggtt
     5161 caggaagaat cgccggacaa attcgctgtg gtcgatacgg tgaaaaccca acccggggcc
     5221 cggaccatga cgctcgacac caggtcgcat aagttatttt tgatcacagc caaccggact
     5281 acgcctcccg cagtcgccgg ccagccggct ccgcgaccgc agatggttgc ggggtccttc
     5341 cgtgtcttag tggtcggcgc ggaatagaac ggaagatcac ggggggcttc aattgcgcgt
     5401 tcgtccccac gtaatgtcag cgcgccatcg aaggttccgt gcgaaggctt ggccgaactt
     5461 cagcgtccag cagcgaattg tctcgtagct gatgtcgagc tcggcgagca tttcctctgc
     5521 gtcccgcaga ctcagcgtga accggaaaat agagccaaac ggcttggcgg atcacgtccg
     5581 ccgggaacct gtggcgcttg aagaagactg gctgcatggg cattccccag tttcggggtt
     5641 catcatcgcc caaaacggag aaagtttaga gttggactga cagcaccgcc agttctctcg
     5701 gcccgccagt tcaatgaaaa ataacgttta ttgactagtt tcgcgctgcc accgcgtttc
     5761 tcgacgcgcg agggtccgaa gcatttgggc aaaccccatg aacgccagca cggcgttgtt
     5821 cctcatcgat gcgcttctgg tctcggtggc ttggccgctg agcgtctggc ttgccacgaa
     5881 tggggcggcg ccccaggaag cgtggttcca ggctctgatt ttcgcgaccg ttaatcaaac
     5941 ctttctctac gcgctgggac tttaccggcg cgaagccctg gtcgatatcg gcaaaacgct
     6001 cggccgcatt ccgctggtcg tcgcgatcgg ggtcgtcacc gccagtttcg cgaccgccgc
     6061 gttcggctgg acgttgtcgc caatcccgta caccgcctcc aatctggcgg cattgttcat
     6121 cgcggcggta gcctgtttca ccgtctgcgc catgctggcc cgtctcgcgt tcctgatgct
     6181 caggcgccat cgcctgttcc gtccacaact gctcgtcatc ggcgcgggca agcgggcgtg
     6241 ggatctgacg tggatgctgc gaagccaggg acgcaacctg cattatgccg tcaccttcgt
     6301 gcatgatgac gtgttcggag ccgtcgatcc gcgcctggcg cgggatccgg cgaatcgcat
     6361 cgtgccggcg tctgaaaacc tgctgcggat cgccgatcgg gtccgtgccg atcaaatcgt
     6421 ggtcgctccc gatgaacggc gcggcatgga tctggaggaa ttgatcgcct gtcggaccgc
     6481 cggctatccc gtgtcgcaat atatgagctt tctggagcag gaagtcgggc gcatcgatat
     6541 caagcggctg gacctgacct gggtgctgta ttccgtgggg ttcgatttcg gtcccgtgga
     6601 ccgcgcgctg aagcgcatgc tcgacattgc cgtgagtctt ttgatcctca gcgtgttttt
     6661 tcccgtcctg ctgctggcga tgctggcggt atggctggaa gatcgcggca cgatgctcta
     6721 ccggcaggaa cgcctgacca aagccgggcg ccgattccag atcctgaaat tgcgcaccat
     6781 gcggatgaat gccgagagcc agggcgccgt gtgggcggcc gcctcggaca aacggatcac
     6841 ccggatcggc gcattcttgc gccgcagccg tctggatgaa ttgccgcagc ttttcaacgt
     6901 gctgatcggc gatatggcgc tggtggggcc aaggcccgag cggcccgaat tcatcgcgga
     6961 gctttcaaag caattgccgc tttaccagga acgccacgct gtgaaggcag gcctcacggg
     7021 ttgggcgcag atcaactatc cctatggggc gtcgctcgac gatgcccgct ccaagctgag
     7081 ctatgacctc tattatgtga agaatttcag cattttcttc gaccttcaga tcatcatgca
     7141 gacgttgcgc gtggtgatct ggccttcggg cgtgcgctag agcgccggtt acggcttggc
     7201 gcgcgctacg gcaagcggtg tgagccggga ttttccggat cgagcttgat cgcctgggcg
     7261 atgtcggcaa tcgcgcgatc atgagcgccc gtctgctcat aggcattcgc gcgctggtcg
     7321 tacgcggcga tgaacttcgg gtctagcgcg atggccttgg tgaaatcctc gatggcgcgc
     7381 gggaaatcgc ccttgtccgc ttcggcgagt ccgcggctga caagcgcggc gacgaatttc
     7441 gcgtcgagcc gaagcgcgga atcgaaatcc tggatcgcgc ggtcatcatc gccctgcacg
     7501 cggtaggcct cgccgcgatt ggagtaggcg acaacggatt tgggattgat cgcgatcgcc
     7561 ttgtcataat cgggaaaagc gtgttccgga tcgtctttca gcagataggt gttgccgcgc
     7621 tgattgaacg cctctccatc tttcggatcg atggcgatcg tctgattgta atcggccagg
     7681 gcgcgctcat agtcgccttg gaactgatag aaatcacccc ggctgcggaa cgcattggcg
     7741 cttttgggca caagcgcgat ggcctgatcg agatcggcga tggcccgatc gttctctgcc
     7801 cgcttccggt aggcaagacc gcgcgtgctg taggccgcgc ttttgaggtc cgccggtccc
     7861 gacgactgaa tgatcgccgt acaaccgatg atcgcgcggg acggatcgag cgaattgcac
     7921 tgcagcatct gcgaactgga cggtccggtg tcgatgttgg gggttctcgc cgcagcctgc
     7981 gccgatggcg cacaaaagac cggcatcgcg gcgataagga gggctgagca tcgcatcgtg
     8041 gtctccaccg cacgcgaaaa tccgatacct acgcatctgg accggcggat atcatagccg
     8101 ttctcacccc agtgcggaac tcggcaatag catctgtttc tgcgcgcttt gcggtggcga
     8161 tgtccttccc gccgaagccg gagcggatga attccccgta gcgcgccaca gccaatcccg
     8221 tgaaatttta tttcggcaag gcggcagagc catctccgga ttgggccgtt tcccgccctt
     8281 gagttgaacc tgtcttgccc ttaccctcaa ccgcactcgc ggggtggccc gcttaggagg
     8341 agctggactc ccatgaccaa ttttcgccgt atgttgaaaa tttcctgttt cgcgctcgcg
     8401 ctgatgccgt ttgcggcatc cgcggagacg gcgctcgtct acgtcaccaa cagcgccggc
     8461 gaccacatcg atgtgatcaa tcctctgacc aataaggtgg tgcagaagtt tccgatcatc
     8521 ggtgcccacg gggtcaactt ctcgcccgat ggatcgcgcg tctatgtcag caacgaatcg
     8581 accagcacgc tcgatgtctt cgaacagaag accggcaagc tgatgaagaa gatcaagctc
     8641 tccaaccacc cgaacaacat cgcggtggcg aaggacggca gaatcgtggt cggcatcgcg
     8701 cgcggccagg gtgcgctcga catcgtcgat cctgtgaagc tggaagtgat caagacgatc
     8761 ccgacgcatg ggcggctgca caattgctac accagcaaag acagcgccta tgcgatctgc
     8821 ggctcggtcc agaccggcat gttcaccgcc ttcgacctga agacccagac catcgcctgg
     8881 gagcataaat tcgatcacgg cgtgcgtccg atgacgatcg aatccaatgc cgacggctcg
     8941 cccaagcgtg tcttcgtcca gctcgcggcc tttgacggct ttgcgattct ggacttcaag
     9001 acccatgagg aagtcgcgcg cgtgaagctg cccgacctca aaaccggcgt ggatatggat
     9061 ccgggccgcg aggatgcccc gtcccatggc atcggcgttt cgcctgacgg cacaacgctg
     9121 tgggtgacgt ccatcccgca gaacgcggtg ttcacctata atctcgcgac acccatcacc
     9181 gccgagtcca agccgatcga caccatcaga ttgccggacc tgcaaattcc gggaaaacgc
     9241 ctgggcggcg ccgtggcgaa ctgggtgacc ttcacgccgg atggccaagt ctacatcgcc
     9301 aattcgggct tgcgctcggt cacggcgatc aacatggcca ccaagaagat cagcgccgtg
     9361 gttccggtcg gcgaagtgcc caagcggatc aatacgctgg tcatgcgctg agcaaagcgc
     9421 aacgcgaata gcaacgggcc ggtgtttctc gcgccggccc gttcgcattt cagaacgatt
     9481 tgggccgcgc tattgcacgc ctacccattg catcaggatc ttgtagtccg gatcgttctt
     9541 gctttcgaat tgacggccgc cggaatggaa caggtcgccg cccgcttcgg gcgcgagcgg
     9601 atgaatcagc agccggcttt cggctttgtt ggccggcgtg accagacggg aaatcgactc
     9661 gaaattcttc cgcgattgtt cttcggtcca gaacttctgg ccttcggcca gcggctccag
     9721 atggaggaaa ttattggcgc cttcatggca attgtagcag ggctcgtgag gcgggcgttc
     9781 tttcaggaag ataggttcca ccttcgtctt gaagaattcg taatcgagtc ccggcgccgg
     9841 cgcggcctgc gacaccccga cggcaatagc cccgattccg agcgtggccg cgactgcgaa
     9901 caaaagcgtc aggcgagctg acattccaat cctccctcgt ttttgaccaa tattggcggc
     9961 aatttagcag aacgctcgcg ccctggcgac gcccattgca agccgcggcg gcacggtttt
    10021 ttgcgtggcg gtgccgctat ctgataaaac ccggcgcacg aggagccgcc atgccccaga
    10081 cctatcatct gcgcgtcaac ggagaatccc gcgaggtcag cgtggatggg gatacgccgc
    10141 ttctttacgt gcttaagaac gacctggagc tgccgggcgc gaaattcggc tgcggcctgg
    10201 cccagtgcgg cgcctgtacg gtcatcatgg atggcgccgc cgtgcggtcc tgcgtcacgg
    10261 aagtccgcag cgtgggaacg cgcgacatcg tcacgctcga aggcatcggc acggagcaaa
    10321 agccccatcc gctgcaggcc gccttcattg ccgagcaggc catgcagtgc ggctattgca
    10381 tcaacggcat gatcatgtcg gccaaggcgc tgctcgaccg caatcctcat ccggcggatg
    10441 gagagatacg cgaggcgctg gccggcaatc tgtgccgctg cggcagccat aatcgcatcg
    10501 tgcgcgcaat tctgcgtgcc tcgaaggagg ccgcaacatg aacaaggtgc tgacacgccg
    10561 cgaattcggc accgcatttg gcggattggt gatcgccttc agcatgggcc cgcgtctcgc
    10621 cgcggccgcg gatggaccgc cgcggcttcc cggtacgctg gcggccaaca agcggctcga
    10681 ctcctggctc cgcgtcgatc cggtgggtac ggtcacggta ttcaccggcc gcgtcgaact
    10741 cggtcagggc gccaccaccg cgctcgcgca gattgccgcc gaagagctcg atatcgcctt
    10801 cgtccgtatc cggatgattc cggtggacac gtcccattcg ccgaacgagg gcgtcaccgc
    10861 gggcagcaat tccatcgaga tgggcggtgc cgcgcttcgt tccgcggcgg ccgaggcgcg
    10921 cgccatcctg gtgcagcttg cctcggagca tctgaatgtg ccggccgcgc agttgatgac
    10981 cgaggatggt ctcatcttcg cccgggacgg tgcacggaag accagctatt gggagctggc
    11041 ggcggaaaat ccgcttgacc gcgaagcgac aaacagcgcg cccgccaagc cggtgaagga
    11101 ccacaggatc gtcggccagc cgattcagcg tctcgatatt cccgacaagg tgatgggccg
    11161 tccggtcttc atccaggata tgcgccttcc cgggatggtc ttcggacgcg tggtccggcc
    11221 gcccaattat ggcgcggagc tgacacggct cgatgacgct gccgtgcgca agatgccggg
    11281 aatcgtctcg gtcgtgcgcg acggacggtt tgtgggcgtg gtcgcggagc gcgaggagca
    11341 ggccattgcc gcgcgcgccg cccttgcgaa ggccgcggtc tggcgcgtgc ccgcgagcct
    11401 gcccgatgcc gcgaacgtcc atgaattcct gaaaacacgc cccggcgtcg tccccaagat
    11461 cgtgaacgag aaaacgggga tggccgcacc cgcgatgaag acgctggaag ctgtctatcg
    11521 caaaccctat ctcgcgcatg cctccatcgg accatcctgc gggatcgcgg gcttcatcga
    11581 tgaagggctg accgtctggt cgcacaccca aggcccctat ccgctgcgga cggacatcgc
    11641 caaggtcacc gcgatgccgc cggaaaaggt gcgcgtcatc cacacaccgg gcgcgggctg
    11701 ctacggccat aatggcgcgg acgatgccgc gctcgatgcg gccctgctgt cgcttgccac
    11761 caatgggcgc ctcgtcaaag tccagtggat gcgcgacgac gaattcggct gggagccgtt
    11821 cggctcggcc atgacgatgt cggcgcgcgc cggtcttgcc gccgatggca gcatcgtcga
    11881 ttggcaatat gatgtgtgga gctgcacgca taacatgcgc cctggcaacc gaagtggcgt
    11941 caatctcctg ggcgcgtggc atttggccaa accgctcgcg cgcgcaacgc ccgccgaggc
    12001 gccgctgccc gccggcggcg gcgaccgcaa cgcggtgccg gtctatgatt ttcccaatca
    12061 gagaatcacc gatcatttca tcgccgatat gccgcttcgc gtttcggcgc tgcgcacgct
    12121 tggtgccttc ggcaatgtgt tcgcgctcga atccttcatg gacgagatgg ccgatgcggc
    12181 gggagccgac ccggtcgcgt tccgtctgaa gcatctgaag gacgcgcgcg gccgcgcggt
    12241 gctgcaggcc gtcgccgaaa aggcgaattg gaagccgggg acaaagggcg acggcgcgcg
    12301 cgggcgcggt gtcgccttct cacgctacga gacgaccaag acctatgtcg cgatgatcgc
    12361 cgacgtggtg gtcgaccgga cgagcggcat ggtgcgggtc gagcgcgtga ccgccgtggc
    12421 cgatgcgggc cagaccgtca atcccgacgg gctgaagaac cagatcgaag gcggcatcat
    12481 ccagggcacg agttggaccc tcaaggagca ggtggtcttt gacaaggaac gcatcgtcag
    12541 ccgcgattgg gtgggctacc ccatcctcac cttcccggaa gttcctgtgg tggacgtggt
    12601 ggtgatcgat cgtccggaat tgccgtcgct gggcgcgggc gaggcctcgc aaggtccgat
    12661 ctcggcggcg atcgcgaacg cgatccgcca tgcgacaggg gcgcgtctgc gcgatcttcc
    12721 cttcacgccg gaacgggcga gggccgcgat tgcgcaaatt tgagcctgcg cctgcatgga
    12781 cgccgcgcat gcatcccgaa ttacggcctt ctgccgtagc ccgagatgcg caatgtctta
    12841 tgcccggttg tgattaagca taagacgatt gcccgctgcc ggtcttgttc aacagacccg
    12901 ccgacgcatc gtaggattgc gcggcaagct gtccgaacat cccccccgtg ttgaaattgg
    12961 actgcgtctg ctgcgccagc gagctgagga agctgccgag ttcgtcaccc gtgaccgatc
    13021 cgtctccgtt gctgtcgatc tgcgaaaaca gcgtcgatga atcggttgag ctggtcccgg
    13081 tcccgctggc cgaactgttc gtattgaaag cggtggccaa ttcatcggac gatattgtgc
    13141 cgtcctggtc ggtatcgaga gcgcccaaga tctgcgtcgc gatatccgaa ctcgaattgg
    13201 cgtcctgact ggaccccgca cccccaccgt gatgatggtg gtgatgtccg gcatgctgcg
    13261 tctgggaaac atcggcaccc agttgctgtt ccgaaattcc ggcggacgca ttctgtccgc
    13321 tggcgtcttg ggcaccggcg ttctgggtca atgacgagaa cagagcatcg gcttgggcct
    13381 gtgtaccgcc ctggctaccg atataggtct ccatttcctt ctggctcacg gtgccgtcac
    13441 cgtttccatc catggctgaa aacagcttct ggacgggatc gttggctgcg ggctgagcgg
    13501 cgcttgcttg ggttccatcg gttggggatt gctgctggag ctgaacaagc aaggccatga
    13561 tctgatcgct gagccccgat tggccgcttc cggtcaatgc ggtgctggcg gtgctttctg
    13621 tcgccgctga actcgaatcc ggggccgccg tgctggaact ggaaccggta ctcgattgtg
    13681 tgctgctggt gctgccgagc cgtgagagca ttcttgcgag gagctggccg gcatcggctg
    13741 ccccagatgc tcccacgccg tttatggaca tggaaatttc tccattaaca aggttacgaa
    13801 cgccggctgc catcgcagac ggcgccgaag gacaaagcat ctttcatgcc cgtggcccgc
    13861 atggcaggca gggcatgaaa tgcatggttt acgagaggtt gcgcggcaag aattacccag
    13921 cccgtttacg agcttcgcca ttcatgggcg gcagattcgt cgcaaaaaat ggcccgaaac
    13981 tgtatcgcgc gataatgtcg gagtcatttt gcggattgtg aaaaacaccc gtgcgattgc
    14041 gcaaaactca gcgcgggtcg tgaagcgcct ggccgctgat cggatcgaaa tccgccactg
    14101 gaaccgtgat gccgtatccg gcattgttct cggcgcacgc ttcttcctcg atgcgcgcca
    14161 cattcacacg cgggtagcgg gtaattgccg accatttcgt ggtgaaggcg ccgggatcct
    14221 cgacggtgaa gaacacctcc agcgtcttgc cctcgtcgat caggcgatag cgctcgataa
    14281 cgtggagttg ttcggtgtgc ggcgtcgcga aacggtcggt ccaggtctta tcgttcaggc
    14341 cgatggtatc gacgatgagc gcgccgtctt cgtaatggcc gacggactcg ccgtaatagg
    14401 agggcgcgag cttcggcgtg tgtggctggt ccagatagac tacgcgcgat tggtgatcga
    14461 actgatagag gaagacgact ttcttcggcg tctgaagaat ctgcatcggc cgcaagaaac
    14521 tcaagatgaa gggcacgccc gccggacggc aagtggtttg cgcggacagt tccggcaggc
    14581 cctgggattc gcgcgcccat tccttgcgca ccgcgtccgc cgcccagggc tgcagaatcg
    14641 gattgctgtc gtcgccgtgc caattcagat tcgccgcgta attctcgggc acgccgatgc
    14701 gccgcacggg accagggccg gatggcggct tgtcatattg ctggcccggc acgccatgct
    14761 gccaaaatcc ggaaaaatcg ggtgtcgcgg acgtcgcgcc ctgcgccgca tcgcaagtcc
    14821 agacgagtgc gaggagcgca attccgctgc gccagccgcg cgatgccgca atcctcatct
    14881 tcgggtttct ttctcgtgaa ggccgcgcac attacccgat tttcatcgat tgccgagacg
    14941 cacgaaacaa tcgcgcgccg cattcgtttg cttttccgga ccaccaagcg ggatcatagg
    15001 catggccacg cgttcaaaga atgccaagac ggacaagccg cgcacacagg acgaaaagct
    15061 cgacgacgct ctggacgcca cgttccccgc gagcgatccg ccgtcgatga ccgaacccac
    15121 caaacatgtg ggcagcggga aaaagaccgg cgcacccacc cccgtgggcg atccgaatct
    15181 gaaataatat tattcctata agattagtag actaataccg ggcctgcgtg tattctcctg
    15241 caaggagata acccgggagc tcgatcatgg cgcaaaccat cttccgttcc gccaatcgcg
    15301 aaacaagcgc attggctgcc accattgccc gcaacatccg gcgcttgcgc tttcgccgcg
    15361 ggcttacggt cgagaagctc gctcaattgg ccgaaatcaa tcttctgacc gtggatcaga
    15421 tggagagcgg caagggcgaa ctgaccctgg cattggcgtg gaagcttgca aatgcgctcg
    15481 acgtcccctt cgcggcgctg atggccgaac atgcgccggg cggtaccgtc ataacgcgca
    15541 aggaccgggc gaaagtcatc gtctcggaga atctcggcgt tgcgtcacgg gctctgtttc
    15601 cgttcgacga agacgcgcgt gtggaattct acgaactgcg catagcgccc gatcatcgcg
    15661 aaacgtcgca gccgcatgcg gtgggaacgc gtgaaatgct gcatgtggca gaaggcgcgt
    15721 tcgaagtcag cgtgggccgc gaaccgccct atcgcgtgaa taaaggcgac accatccact
    15781 ttctggcgga tctgccgcac agctatcgca atctgacggc aatgccggca accgcctatt
    15841 tggtgatgac ctaccaatag agcggcgatc cggtctaata tgcgtgaagg ggttcgccct
    15901 ttcgaaccat tttccacacc ggttggaggc tttcatggca ttgcggctgg gcgacatcgc
    15961 cccagacttt caggcggaca ccacgcaggg gcccatccgc ttccacgaat ggctcggcga
    16021 ttcctggggc gtgctgtttt cacaccccaa ggatttcacg ccgatctgca ccaccgaact
    16081 cggatacatg gcgcgcatca agcccgaatt cgacaagcgc aatgtgaaga tcatcggtct
    16141 ttcggtcgat ccggtggatc gtcattccgg ctgggcaaaa gacatcgaag agacgcaggg
    16201 caccgcgccc aattatccga tcatcggcga tcccgacctg gcgatttcca agctctacga
    16261 catgctgccg gcggaagctt ccggtgaccc ttccaagcgc accgcggccg acaaccagac
    16321 cgtgcgcaac gtctacgtca tcggtccgga caagaagctg aagctcatcc tcatctatcc
    16381 catgacgacg gggcgcaatt tcgacgaagt cctcagagtg atcgattcca tccaactcac
    16441 ggccaggcac aaggtcgcga ctccggtcaa ttggaagccc ggcgaggatg tcatcatctc
    16501 gggatcggtg tcggacgagg acgccaagaa gacctatccg caagggtgga aggcccccaa
    16561 accctatatc cggatcgtgc cccagccgcg ataggcccct cagcccaccg ccacgtcgga
    16621 gaagcggtcg acccgccgga gcggcggaaa gagcgccatc cacaggccga cgaccaggac
    16681 ggtcccgata ccgccgagca ccaccgccgg cacggtcccg agcgcgcctg cggtcatgcc
    16741 ggcgcggaat tcgccaagct cgttggaggc cccgatgaaa agcgaattca gcgagcccac
    16801 acggccgcgc atgttgtcgg gcgtggcgag ctggatgagc gcggaccgta catacacgct
    16861 gaccatgtcg gccgcacccg agaccgcgag cgccaccatg gagacgtaga aatcacgcga
    16921 caggccgaag acgatggtcg cgatgccgaa gatcgccaca cacccgaaca tggagatgcc
    16981 ggtatgacgg tgcaattgcc agcgtgccag cacgaaagcc accagcgcag cccccatggc
    17041 aggtgccgtg cgcaggaacc ccagtcctgc cgggccggtg tgcaggatgt cgcgggcgaa
    17101 gatcggtagg agtgccgtgg ttccgcccag gaacatcgcg aaaagatcga gcgagatcgc
    17161 ccccagcaga atggggcggg cgcgcatgta gctgattcca tcgacgatgc gctgaagggc
    17221 gttgccctgg ccggcgctgc tctgctgggg acggatccgc agaatcgcca tgccgacaag
    17281 cgtgaagatg tagaggcaaa ggcaaagact gtagttcacg accggcccca gatcgtacag
    17341 gaagccgccg accgcggggc cgccgatcac cgcgatcctg taagcggagg cgttccaagc
    17401 gatcgcctgc ggcagcttgt ccagcgtgac caggaaggga agaaaggatt gcatggcggg
    17461 ctgcgaaaga ccgagcgcca cgcctaataa cgtgatgacg ctgtagaatg cccacatcgc
    17521 tttcaccccc gtaagagtga gacccagcag catcacggag gcgacgacct gcacgagata
    17581 gcttgccatc agcatccggc gccgatctac ccggtcggcc agatcgcccg cgggcagcac
    17641 gagcagcagc atgggaagaa agatgctcag gccgacatag ccgagcgcca caggatcgcg
    17701 ggtgaggtcg tagacctgcc agccgatggc gacggactgg atctgggttc cgagcgtggt
    17761 gccaaaccgg atcgcgagat agagccgaag gtcccagctc ctgggcttga actcctcggc
    17821 ggtgaagagg gggttaggcg tgctcacggg aagggatttt taaccgggga tagggttaat
    17881 gcgatcttct agggctactg gctttcttgc acaattgttt gtgaagcaaa atcattccga
    17941 atgcatcccg taacggtacg gccattttcg aaatgtccga atgcccgcgc cgcaacacga
    18001 attcacccgg ccgacaaagt tttgggacag atggcgatta tgtccgagac gtggatattt
    18061 gtgttagaaa ccctgctcgg gcacgctttt gctttgtctg ccgtgttcct tcgggctgaa
    18121 agaaaatgat gggatacgac gaaccttcct caaagcgcca tgccacggtc tatggaacac
    18181 atcccgccgt gaagcaacgc aacccgtggc cgcgccgtat cttgctcgcc gtcattgcgc
    18241 ttggagtcgg cggttacatc gctgccaatc cggacgtcgt gcagaagatc agctcgctca
    18301 ccacgtccgc tccggccggt ggacgcggcg ctgcgcgcaa cgcaggagca gtcccgccgg
    18361 tgcgtgtcgc aatggcggaa acccacgatg tccccgtagt ggtccacacg atcggaaccg
    18421 tgctcgccaa ttccatcgtc accgtgaaat cgcaagtgga tggtccgctg ctttcggcga
    18481 tgttccgcga aggacagatg gtcaagaaag gcgatctgct gtttcgtatc gaccccgcgc
    18541 cgttcgaggc ggccttgcgc cagtccgctg ccatggtcga acgcgatcaa gcgcaatatg
    18601 acagcgccaa ggcagacgcg gaccgcgccg tgatgctcgc cgatcgtggg atcgtggcgg
    18661 cacagcagcg cgaccaattg gtcgccaccg ccaaagcgct cgccgccacc gtcgccgccg
    18721 acaaggccgc ggtcgagcgc gcccagctca atctcgacta caccacgatc cgctcaccca
    18781 tagatggcaa gaccggcgcc ttcatcatct atcccggcaa tcaggtgcat gcgagcgatg
    18841 cggccggcct ggtgaccatc acccagattc agccggtcaa gatctccttt ttcctgccgc
    18901 agggcgacct gccgcaattg caagaccgga tgcgtgaaaa tcagcttgtg gtggatatca
    18961 cgacccgaag cgacgtggcg gcagaaccgg cggccgcgcc cgcggcagcc accgccggcg
    19021 catccgatgg ggacattccc gtaaaggtgg atttcatcgg caataccgtc gacgataaga
    19081 ccggcacgat cgaattgcgt gccacgttcc agaatcccga tctgcggctg gtgcccggcc
    19141 aactcgtcga cgtcagcgcc aatctcgaaa ccttcaagaa tgtggtgacg gtgccgcgcg
    19201 acgccgtcaa tgtcggccag gacggtaatt acgtctacgt tatcgacaag gacatgaagg
    19261 cgcagatgcg gccggtcaaa gtcctgtacc aggatcaggt catggccgcg ctcggctccg
    19321 gcattgccaa tggtgagcgc gtggtgaccg acggacaatt gcgcgtgacg cctggaacca
    19381 cagtcgccat cacgcatccg gaaggcgaaa cccccgccaa ggccgcgccg caagcgaaca
    19441 cggacgcgag cggcaccgga cgcggggggt aaagcggcgg ataggcaagg ctagccagga
    19501 tgcatatctc agcggctttc atcaaacgcc ccgttatggc gactttgctg atggcagggt
    19561 tcgttcttgc gggtctgttc ggctattcct cgctgccggt cagcgagctt cccaacgtcg
    19621 attttccgac catcgacgtg aatgccagct tgccgggcgc agacgcgcag accatggcgt
    19681 ccgcggtcgc cacgccactg gaaaagcagt tctcgctcat tgccggcctg gattcgatga
    19741 cgtcccagaa cgctcaggga cagacgcgca tcacgatgca gttccggctt gaccgcaata
    19801 tcgactccgc ggcgcaggat gttcagtcgg cgctctccgc agccgcgcgg cagctcccgc
    19861 aggccatgcc gacaccgccc tcgatccgca aagtcaatcc ctcggaaggg tcgattcttt
    19921 tcttgaccgt gagttcggca acgctgccgc tgtccgaagt caacagctat gtcgaaaacc
    19981 tgctgattgg aaaactctcc tcgatcaacg gcgtggccca ggccgatatc ttcgggcagg
    20041 cgcgtcctgc ggttcgcgta caggtcgatc ccaacgcgct agcgctgcgc ggcatcggca
    20101 tcgacgaggt cgccaccgcg atctcgcgca gcagcgtcaa tatggcgacc ggccagctcg
    20161 acggaaccac gcggggcgcc gtgatccacg ccgacggtca gctcaacaac gccgagcaat
    20221 acgcctccca gatcattgcc taccgcaatg gggcgccggt ccgctttgcc gacgtcgcga
    20281 acgtcatcga cggcgtcgag aacccgcgga attatggcgg ctggggcacc aaagagggca
    20341 ccattccggc ggtgaccgtt ggcatcgacc gccaacccgg cgccaacacc gtcgcggtgg
    20401 tcgacgcgat ccgggcggcg ctgccgcagc tcgtcgcgca gcttcccccg tccatcaaag
    20461 tcgatgtgac actggaccgc agtaattcgg tacgccaggc ggtctcggat gtgcagttga
    20521 ccttgctcat cgccgccttc ctggtgatcg gggtcatctt cgtctttctg cgcaccgtct
    20581 cggcgacatt catcccggcg atcgcgttgc ccatcgccat catcggcacc ttcgccggga
    20641 tggcggtcat gggctacagc ctcgacaacc tctcactgat ggcactgacg ctgtgcgtcg
    20701 gcttcgtggt cgacgacgcc atcgtgatgc tcgaaaacat catgcgccac gtggaggcgg
    20761 gcgaagaacc ttatcaggcg gcgatgatcg gttcgaagga agtcaccttc accatccttt
    20821 cgatgacggt gtcgctcatc gcggttttca ttccggtcat cttcatgggc ggaatcgtcg
    20881 ggcggctgct gcacgaattc tcgatcacca tcgccatcgc ggtcatcgtg tcgggcatcg
    20941 tgtcggtcac actgacgccg atgctctgca gccggctgat caaatcggcg aaggaagaac
    21001 acagcaaaca ccacaatgtt ttctaccggg tgagcgaaag aggcttcctg gcgatgcagc
    21061 gcggctatga acgcagcttg cgctggacca tgagccaccg ccccctcatc ttcgtgatct
    21121 tcctgctcag catcctcgcg acagtgcaga tgttccgcat catgccgcag gatttcttga
    21181 ccgccgagga cacagggcag atcaacgcct ttacggatgg cgccaacggc gtctcgttcg
    21241 cggagatgcg ccgccatcag gacgaggccg cgcggattct tgcggccgat cccaatatcg
    21301 acggcttcat gacctccgtc ggatcgggtg ggacccgggg cggcctgaac tcgggcggat
    21361 tccgcattca gctcaagccg cgcgatcagc gcccgcttgg ggtcaacgat gtgatggcgg
    21421 agctgcggca gaaatttcaa cgcattccgg gcatcaatgt ggtgatgcaa aaccgccccc
    21481 cgatcaacat cggcggatat gtcagccgcg ctctgtacca gtacaatctc caggacatcg
    21541 accttaagga gctttatacg tggtcacagc gcctgcagga ggcgttggcg aagaatccga
    21601 atttcgttga cgtgaacagc gatctcgatc tgtccacccc ttcggtcgag gtctcgatcg
    21661 atcgcgacaa agccgcggct ctcggcatca cgacccagca gatcgaaacc gcgttggggg
    21721 ccgcgttcgg tgggcagcgg gtggcgccga tctatacgca ggccgaccaa tattgggtca
    21781 tcctggaatt gctgccgcaa tatcagcagg acgcggacgc cttgagccgg ctttatcttg
    21841 ccacgccgcg caccgcctcg gtttccacga gcaacgtcgg cgcgtccgtg ccgctttccg
    21901 ccgtgacgac gatccgccgc ggaacgcagc ctctgtcgat caaccattcc ggccagcttc
    21961 cagccgtgac actgtccttc aacctgcctc aaggtgtcgc gctcagcaac gcggtggcgg
    22021 cgcttgaaca gatcaaacgg gacatcggct tccccgcctc cgttcagggc aatttccagg
    22081 gcaccgccaa agcgttccag gattcccaga aaggaatggg gttcctgctg atcggcggca
    22141 tcctcgtcgt ctacatcgtg ctcggcattc tctacgaaag cttcattcac ccgcttacca
    22201 tcctcaccgg cctgccctcg gcggcggtgg gcgcgttgat caccctgtgg ctgttccact
    22261 attcgctgac gctttatgcc tatgtcggca tgatcatgct ggtcggtctg gtaaagaaga
    22321 acgcgatcat gatggtggat tttgcgctga cgcggcagcg cggcgaaggc atcgaaccgg
    22381 aggccgccat cgtggatgcc gccgtggttc gcttccggcc gatcatgatg accaccatgt
    22441 cggcgcttat gggcaccttc ccgatcgcgg tcggctgggg ctccagcagc gagggacgca
    22501 agccgctcgg ccttgccgtg gtgggcggtc tgatcgtgag ccaggcggta acgctctata
    22561 tcaccccggt gctgtatgtg tatctggaca agctcgggtc cgcgcttggc agaaaacgca
    22621 aaccgattgc gatcccggcg gaatagagct tcagattctc aggagatgat tgtcgaacgc
    22681 ggcaggcgcc cgccaatgcg cgtcagcctg caatcccgca tcgctcgcgc gcagcttcat
    22741 cacatcgccg agacccgaag tggcccaaaa acaagccgct tgcgcgtctg ccgcgaggcc
    22801 gcagacatcg gccgccgtat agccgccgag ccagcgctca tcgccgagcg accagagatc
    22861 gacccggccg cctttcggac tggcggcggc agcgaagcgc cctgaactgt cgatggccac
    22921 cgagccgaca taggaacgca ggcttccctc atcgtcgccc ggcagcggca ggagcgcgat
    22981 atcggccccg atgcgcagga tccccatcaa cggacgtaat tgtgcgcggt cctggtcctg
    23041 catgccgaag gcgagcgtct cgccgtcggg cgcgagcgcc atgtgccgca gcgacagcga
    23101 tttcaacgat gggccgagcg tgtggcgttt cagaatggcg cccgtttcgg ggtccagctc
    23161 gagaatcgcg ctgtcgatat tccccgcatt gatgggtggg cccttcacgt cggccgcctt
    23221 ggcaatgccg ccaatcgcca ccatcagacg tgcgccgcca cgcgcgaaca aaagatcgtg
    23281 cggttcgatg ccgaccggga aaatcgcgcg cgggcggccg tcttgcgcat cgcgcaccac
    23341 caccgcacca gccccggtct cggcgtcgat ctcgctggtg acgagcatac gttcgtccgg
    23401 gtggagcgcg gcatgcccgg cgaagcggtg gccggcggcg ggatggaaga tccgtggctc
    23461 ggcttgcggc atctccaggt tcacgatggc cgcgaactca cccggccggc ggccgaccag
    23521 aagcacctcg cctccggaaa gcgccgtcaa cgcatgcgcc ctgcccggca aggaaaaacc
    23581 cgacacgcct tgcgcatcga tgcgtatgcc gccatcggtt tcaccgatcc gcgccgcgct
    23641 gacgagcgac acagcaggcc tgtctgcggc tgacgcgcgg gtcttagacg cgaaagtccc
    23701 gcccagaagg gccgcggcgg cgcctttgaa aagcccgcgc cggtgaatcg tccaatcaat
    23761 cgccatcgag actattgaac cccaatgtaa ccccgacatc gccgggaagc gttttcgcaa
    23821 tttcggtctg caccgatttg atcgccgcgc gtccggcatc cacgcgcgca cgccgtttcg
    23881 gatcggccgc ggccgcaccg gcatcggccg gcatggcatc gaccgccttc accgcggcgg
    23941 caaacaagga acctagtttc gcgcgatgag cggccggcgc ggtggccgcg aaagccgttt
    24001 cccattcctg cgcggcggcc aaattgagcg ccaggtcacg ctgggtgcgc ccgctgcgcc
    24061 aggcttcggc cgattttggc ttcgcggcgg cggcgttggc gcccagaacg ggcagcagct
    24121 tgacgtcgtg catcaatttg aacgcggcca cgagatccgt cagcagaagc cgggcggctt
    24181 ccggcgcgtc ggcgaagaga ttcttccagc ccttgttcgc cgcaatggcc gcgcgcacgc
    24241 catccggcgc cgtccaatcg gcaagcacct cgcgcgcgat ggccgaaaga ttctgcgcga
    24301 tggcgacgcc gacctgcgtg cgccagacgc cgtccttgcc cggcgccttg agaagctttg
    24361 ccgcatcgtc gtcgtaaagc agccgctcaa gcgccgtcag cccctgtccc gccacgctgt
    24421 catgggcgag cgagtccgcc gtgagatcct tggggtcctt ggacatcagc aattggtcga
    24481 gcgcgcgttg ggtcgcgttg cgtgcttcgg gccaataggc aaagcggtca tagcgcagaa
    24541 acagggtgat cggccccgtc ttgatgagtt gggcttttgc ccaggcatcg gcgtccgcat
    24601 tataggctgc gcgcagggac gtaaaatcgc cggcctcgcg cttggcggca aaggcaaccc
    24661 aggccgcttc ctgcgccgcg ctcgcttgga caagcgcgcg gtaggttggg atgacaaacc
    24721 gatcggtgac cgcgagcgcg attttccccg cctgcacggc ttttggatcg ggctttgcgg
    24781 gcgcggactg tgcggcggcc tttgcaggca ccggtgcggc ggccagaaca agcagcgagt
    24841 tgcgggagac gttcaccatg gcttcccttt ataacccgct tacgaaggcg acaagcgcgg
    24901 ccttatccga ttgcgtcagc gcgcggaaac ggtcgcgtgt tcccctcgcc tccccgccgt
    24961 gccattcaat agcctcggtg acatcgccgg cgcggccgtc atgcagcaat ccgccggttt
    25021 tcaggcttcc cgccacgtcc cagagcggtg ccgtccgcca gaaccaggaa tcggccgagc
    25081 cttcctcgat gccatcgccc agttccccgc ccatgtcgtg cagcaaaaga tcggtgtacg
    25141 cgggaacggg cttgccattc gccagatgca gcgtggcgtg gcagacggca cagccgatac
    25201 tgacgaacag cgtcttgccg gccgcgaatt tcgcgggcgc aggctgcggc ggcgacaaag
    25261 ccgccacata acccacgatc agatcgaaca gcggatcggc gacttccggt gcgccatcct
    25321 ccgacccatg cggtccggca cggcaggcgc gctcggccgc cgtacaatcg ccccagggct
    25381 cgggacgcag acgcgtcgaa agcccgatgt cgcgcgaaaa tgccgccgca acctgcgccg
    25441 gcagatcggg ctgtgttgcc ttccatccga accgccccag gacgcgcttg ccgtcgatcg
    25501 tgacccaggg cacgcggcct gcgatgccgt catggtccgc atcttccgaa tcggcgcggc
    25561 tcaggatttc gctttccgga atttgcgcga gcaggccggc gcctttgagc gagggcgcgc
    25621 ggcgcaacga caatctggtg tctttggcga gcggcccgta gccaagactg ccgggaacga
    25681 tcgttgggat tctcagatcg cctttccccg tccactggat ggcgggagcg gcctccggaa
    25741 cctggcctgc gacaccgtgt gtttgcaatt gcacgccata gaccggatcg ccctcgccgc
    25801 gcccattgcc gagccggatc accattccag cgggcagcgc gctttcactc gccctgtcgc
    25861 gcggattgga cgtgtggcag gccgcgcagg acatcgcatc gtagaggggg cccaagccgt
    25921 cgtccgattt ggtggaggat ggcgccgaca cccaattgcg ttcgaacaga tgtttgccga
    25981 tggcaagatc gagcgaggct ccggtgagca gcggagcggc ggccgcaaga ccgagggctg
    26041 cgatcaaacg acccggaacc accccatcat ccccgtgtct tgatgttcga tgatatggca
    26101 atggatcatc caattgcccg gattgtccgc aacgaaggca atctcgaccc gttcctccgg
    26161 caataccagc acggtgtcgg cccaatgcac cggccgcttc attttgctcg ccgacaacac
    26221 cttgaatgta tggccgtgca gatgcatcgg atgcatatgc ggcgtggtgt tgttgaactg
    26281 aagccgaacc ggggttccgc gtgaaaagct catcagcggc ggcggcaaat gctcgtggcc
    26341 gttttgcggc caggtctttc catcgatagc ccagagtgtt tgcgcggtgg agcacagcga
    26401 atccagaacg tcgattttgc gcccatcggg aagcacaatg ggcgcaagtc cggcataatc
    26461 ggtcggcgtg gccgaggccg acagcttcaa ttcatgggtc tccgccttgt cgagatcggg
    26521 ctccggaagc cgggcgggcg ccagagccgg acccgtcgcg gagctggctt ttgacgcacc
    26581 ctgggcgacc agcgtcgcca aggtcaccgg ctgggccgcg aaataatcga tcagcgcgat
    26641 cctgccgccc gcatccggcg tgcgcagcgc cacatccatg cgcatggccg gacccatgcg
    26701 ccagctctcc agcgcaaagg gcgtgagcgc attgccgtcg accgcgatga cgcaagcccg
    26761 cgcgccctga acgccgatgt cgctgatgcg cgtggaatcc agattgagaa ccctgaggcg
    26821 gatattcgcg ccggccggaa ccgtgatctc gggcgccacc tgcccgttca ccgtgcgcaa
    26881 cgtgccgaat gtgccgccgc gcgccgcgcc ttgcggtgtg atgaaggcga ggaattgccc
    26941 gtccttcgcc acgcgccaat ccttcatgac acagacgaca tcgtcgtcga acgcaacacc
    27001 gtcctccacg atgaggacgc ccgcaagtcc gcgccccaat tgctcggccg tgttgcaatg
    27061 cggatggaag aaataggtgc cggggtccgg cggagtgaag cggtacacaa aattctcgcc
    27121 cggctctacc ggcatttgcg tcacataggc gacaccatcc atcgcattag gccctcgcac
    27181 cccgtgccaa tggacggagc tgtgctcctt caggcgattg gtcagcgtcg cttccacact
    27241 ctgacccaaa tgcagccggt acactggaaa aggcgcgttg ccgtaaagcc atgccggcgt
    27301 caaccctgta gaggcgccca gcatcacagg ccgctcctga gcgacgagag aggtgggcac
    27361 accgccgatg cgatattcgg gcaatcggcc aacgggcact gtcctggcgg accgcgcaaa
    27421 acggccgcag gatgcgagcg tcaccgcggc caacacgccg ccctgcgcca tcaatgcgcg
    27481 gcgcgtgagc gcggggcgcg tgcccggcat cagcgtctca ccgcgtgacg gcggacggat
    27541 tgtcgaggct gtccgatttt tcgagcgata tcttcagacc caaagctgcg accacagcct
    27601 catccgcgcg ggtttgcgcg atcagcgcat cgatggcgtc ctgtaccagc ctgttgccgg
    27661 cggcattgtc cttcgcgatc atctggtcgt aggccatctt gccggaatcg cccatgtcct
    27721 tgatcgattt tatcttcgcc agcgtgtccg cgattctgtc gtccatgcgc ttggcgacag
    27781 caggattttt tgcgaccacg tagtcgcgga aactcgcacc ctggaccatt gtgccgttca
    27841 cccgcgtata gcggccacgc cagatctcat tgatcccaag ttcgtcgtaa tagtgtgaat
    27901 tatgcgtgtt gtcggaaaag cagtcctgct cttcttccgg atcgtgcagg ataagaccga
    27961 gcttcatgcg ttcgccggcc atctctccat aggacagact gccgatcccg gtaaggaccg
    28021 tggaaagccc gccattctgg cctttcgcgg ccagatcctt gcgggccttt ccgcccgccg
    28081 cccaatccgc cgtcatctcg gccatatcgt cgaccagaag atcggtcgcg gctttcagat
    28141 attgcgcgcg gcggtcgcaa ttggcgtgcg tgcagttttt ctgcgaatag tcgctggccg
    28201 gtctttcacc cgcgccgggt ccggtgccat gcagatcctg gccccacagc agaaactcga
    28261 tggcgtgata accggtggcg acattggctt cgacgccttg ggcctgttgc aattgacgca
    28321 ggagattgcg gtcgatgacc gatgcgttga cctgcttggc gccgatgcgg atctgcctat
    28381 tggcgatgac gttcagcgtg tacagaggat tttcgtcgga ggtcttgcca taggacgccg
    28441 cgacgtaatc gatcaacccc tcatccagcg gccaggaatt cacctcacct tcccattcat
    28501 cgacgatggc gttaccgaaa cggaaagctt cagtctgaag ataagggacc cgggctgcct
    28561 tccaggcgtc acgcgcagtt ctgagcttcg caggcgtggg gtccgcaagg aacgcgttca
    28621 ccgccccttg cagggccttt gccgtgataa aagaatcctc gtacatcgca tgcgcgaggt
    28681 cgccataggt gttgagcaca tccacgggct tgggctgcac cgcggatgca gcgagggccg
    28741 cgcccgagag cagcgcaccg gcaacggaaa atgcgaaaag cctgcgcatg gaaaggtccg
    28801 ttctgaacgt ccatgggaac cacatatgcg actgatttgc aagtgcagct aaaatgacaa
    28861 tggatttagt attccgatcg gcgaacagtg aaaagaaccg gcacgaacag gggacatgat
    28921 gatgaagctt tataatttcc acgccagcag tacgtcctac cgcacccgga tcgtgctgaa
    28981 cctcaagagc
//
LOCUS       DASYEG010000189         4263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_931151, whole genome shotgun sequence.
ACCESSION   DASYEG010000189 DASYEG010000000
VERSION     DASYEG010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931151"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VGM72_08710"
     CDS             <1..666
                     /locus_tag="VGM72_08710"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEY4265385.1"
                     /translation="DGSKLRFNTPTVFNAVLNFRLNWVGNFRTLESQAASSIENPRIM
                     GSSLEEVVGKIASDPGLTWQFRTAYGHGPDVDSLLDALAAFERTLVTPDSRFDRWLAG
                     DASALSAEEINGYRLFKSLGCVSCHQGRNIGGNLFQRHGIFHPLDSPLPEILRVPSLR
                     NIATTPPYFHDGSAPTLENAVRRMAYAQLDTSLTQEQVHDIVAYLRTLTGTFRGKVVG
                     DQR"
     gene            663..3164
                     /locus_tag="VGM72_08715"
     CDS             663..3164
                     /locus_tag="VGM72_08715"
                     /inference="COORDINATES: protein motif:HMM:NF010411.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system VirA-like sensor kinase"
                     /protein_id="HEY4265386.1"
                     /translation="MRIILAAMGVALLLLLLTWLLLRSTSPEIHTDQIAFDALDDMAT
                     SESALHRDMLRARAGMLNNYDPLVAEEDAIRDSISRLETAVRNDPQISDAVDQLAMTA
                     DLQEEWTEEFKASNALLHNSLAYFRLFSAELSESPRDPVLARQVSGLTAAMLHLTLDT
                     SSSVMDEVDDELNRISTENLSPEDTRAVPAMLAHARMLRDLLPATDHTLKALFATRSH
                     QQGQAIRVLVLARAAAFEQRASRYRYVLYGISLILVAMLIYLGVHLEWRARLLRRRAN
                     FEHIIARISTRFINAGPGELGAQVARALEEMAAFFGADRAYFIVTDGAGQIYRWSRTG
                     IMFPHGWPQKALAVASRIGGWSNGIVHIAHDDQLAHGDGSGVPAAIGIREWLCIMDPK
                     GEPFGGMLGFDALTGKIGIRKSEFGLCRMAFDAIAYAIDREFLEGERRRLEDKLQQAH
                     RMETIGALASGVAHNFNNIVAAILGYAEMAHAQVKPGSRAAENVAEIRQAGERARDLV
                     EHILTFGRRSDTSREHIPLGTLILETKSFLEATLPTRIKLVVGQVSDQSILISGELAQ
                     LQQVIMNICSNAAQAIDGAGTIEIEAGLTEVAAPLQLDHGRLAPGRYTVISVSDDGRG
                     MDEATLERIFEPFFTTRADGNGLGLSTALEIVREHGGGIAVESKPRIGTRFEVWLPYS
                     PAIQADTEQRAQRVAGRGGGQAVLVFEADRARLLKHEEILAALGYEPAGFTDVSEAIA
                     ACRASPGRFDIALLCHQHGKGSPLDLAMTVHRAVPDLSIILAVPWAADLAAPALAEAG
                     VSEIVGLPLTSAELSGALAHCCETPLSRRRMREEA"
     gene            complement(3257..3694)
                     /locus_tag="VGM72_08720"
     CDS             complement(3257..3694)
                     /locus_tag="VGM72_08720"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY4265387.1"
                     /translation="MLESAYQLFSQQGTRSVGIDTIIQHSGVAKMTLYRHFQSKQELV
                     AAFMELRHELWTQDRLCSEVKKRANTPRNRLLGIFDVFDEWFNRDGFEGCAFVKALLE
                     YQPGDPLHGAAVARRASASLFMSWRRRRALTIPRLSPTPGTSL"
BASE COUNT          822 a         1262 c         1333 g          846 t
ORIGIN      
        1 gacggttcca agcttcggtt caatacgccg accgtcttca atgcggttct taatttccgc
       61 ctcaattggg ttgggaattt tcgaactttg gaatcgcaag ctgcctcttc gatcgagaat
      121 ccgagaatca tgggttccag ccttgaggag gtggtcggca agatcgcttc ggatccagga
      181 ctgacatggc aattccgcac cgcttatggt catgggccgg acgtcgatag tcttttggat
      241 gcgctcgctg cttttgaaag aacgctggta accccggaca gcaggttcga tcgctggctc
      301 gccggcgatg cgtccgcatt gtcagccgag gagataaatg gttatcgcct gttcaaatcc
      361 ctgggctgcg tgtcctgtca ccagggcagg aatatcggcg gcaacctttt ccaacggcat
      421 ggaatatttc atcccctgga ttctcccttg ccggagatcc tgcgcgttcc gagtttgcgg
      481 aacatcgcga cgacaccgcc ctatttccac gacggcagcg ccccgacctt ggagaacgcc
      541 gttcggcgta tggcatacgc ccaactcgat acttcgctta cgcaggaaca agttcacgat
      601 atcgtggcct atctccggac tctgacgggt acattccgcg gcaaggtcgt cggagatcag
      661 cgatgagaat cattctggcg gccatgggag tggcgcttct tctcttgttg ctcacctggc
      721 tgttgctgcg cagcacgagt ccggagatcc ataccgacca gatcgcgttc gacgcgcttg
      781 acgacatggc gacatcggaa agcgcgttgc atcgcgacat gctccgcgcg cgagccggca
      841 tgctgaacaa ttacgatccg ctggtggctg aagaagacgc gatacgcgat tcgatttctc
      901 gtctggagac ggctgttaga aacgatccgc agatcagcga tgccgtggac cagctggcaa
      961 tgacggcgga tctccaggaa gagtggacgg aggagttcaa ggccagcaat gctctgcttc
     1021 acaattcgct cgcctatttc aggctgttca gcgccgaatt gagcgaatcg ccgcgggatc
     1081 cggttttggc ccggcaggtg agcggcctga cggccgcgat gctccatttg acactcgaca
     1141 cttcgagctc ggtgatggac gaggtggacg acgaactcaa ccggatatcg accgaaaacc
     1201 tctcacccga agacaccagg gccgtgccgg ccatgttggc gcacgcgcgc atgctgcgtg
     1261 acctcctgcc ggcgacggac cataccctga aggcactgtt cgcaacgcgg agccatcagc
     1321 aagggcaagc catccgggtg ctggtgcttg cccgtgccgc ggcgttcgag cagagggctt
     1381 cgcgctatcg ctacgtgctc tacggcatat cgctcatcct tgtcgcgatg ctgatctatc
     1441 tgggagtgca tctggagtgg cgcgcgcgcc tgctgcggcg gcgcgcaaat ttcgaacaca
     1501 tcatcgccag aatctcgacc cggtttatca atgccggtcc tggggaactg ggagcgcagg
     1561 tcgcccgcgc gctcgaagag atggcggcct tttttggcgc agatcgcgcc tatttcatcg
     1621 tgaccgacgg cgcggggcaa atctatcggt ggtctcggac gggaatcatg tttccgcacg
     1681 gctggcccca gaaggctctg gccgtggcat cgagaatcgg aggctggtcc aacggcatag
     1741 tccacattgc ccatgacgat cagctcgcgc atggcgatgg aagcggcgtt ccggccgcga
     1801 tcggaattcg ggagtggctc tgcatcatgg atccgaaagg cgagcctttt ggcggaatgc
     1861 tcggcttcga tgcgctcacc ggcaagatcg ggatccgcaa aagcgaattc ggactctgcc
     1921 gaatggcgtt cgacgccatc gcctacgcca tcgaccggga gttcctcgaa ggcgagaggc
     1981 ggcgcctgga ggacaagctg cagcaggccc atcggatgga gaccatcggc gccctggcga
     2041 gtggggtcgc gcacaacttc aacaatatcg tggcggccat tctcggctat gccgaaatgg
     2101 cccatgccca ggtgaaaccg ggaagccgcg cggccgagaa cgtcgccgag atccggcagg
     2161 ccggcgagcg cgcccgcgat ctggtcgaac atatcctcac tttcggccgg cgcagcgaca
     2221 cgagccggga acatatccca ctgggtactt tgatcctgga aacgaagtcc tttttggaag
     2281 cgacgcttcc aacacgtatc aaactggtcg ttggacaggt ctcggatcag tccatcctga
     2341 tatccgggga actggcgcaa ttgcagcagg tgatcatgaa tatctgcagc aatgcggctc
     2401 aggcgatcga cggggcggga accatcgaaa tcgaggccgg gcttacggaa gtcgcggcgc
     2461 cgctgcagct ggatcacggc cggctcgctc cggggcgata cacggtcatt tccgtgagcg
     2521 acgatgggcg cggcatggac gaggcaacgc tggagcggat cttcgagccg tttttcacaa
     2581 cccgggccga cggcaatggc cttggtcttt cgaccgcgct ggagatcgtt cgtgaacatg
     2641 gcgggggcat tgccgtcgaa agcaagcccc ggatcggaac ccggttcgaa gtctggcttc
     2701 cctatagccc ggcgatccag gcggatacgg agcaacgcgc gcagagagtg gcgggccgtg
     2761 gaggcggcca ggctgtgctg gtgttcgaag cggaccgcgc ccggctgttg aagcacgaag
     2821 agattctggc ggcgctcgga tacgaaccag cgggtttcac cgatgtatcg gaggccatag
     2881 cggcttgccg agcgtcgcct ggacgcttcg acatcgcgct gctctgtcac cagcacggca
     2941 agggctcgcc gcttgatctc gctatgacgg tgcaccgggc ggtaccggac ctgtcgatca
     3001 tcctagcggt gccatgggct gcagacctgg cggcgcccgc attggcggag gccggcgttt
     3061 cggagatcgt cggattgcct ctgacgtctg ccgagctttc cggcgcgttg gcgcattgct
     3121 gcgaaacgcc attgtcccgg cggcggatgc gtgaagaggc ttagaaaccg cgccagtggt
     3181 cgagaatcaa ggtcgccgcg cttcgcgtcc ctcgcggccg agcggttacc ttccgtcgcg
     3241 gcgatgatcg gccctttcat aaggatgtgc ccggtgttgg cgagagcctg ggaatcgtca
     3301 acgcccgccg ccgtcgccag ctcatgaata aagacgcgga tgcgcgacgc gcgacagcgg
     3361 cgccatgcag cggatctccg ggctgatatt cgagcagagc cttgacgaag gcgcagcctt
     3421 cgaacccgtc gcgattgaac cactcgtcga agacatcgaa gattccaagg agacggttgc
     3481 gcggcgtgtt cgcgcgcttc tttacctcgg agcacagccg gtcctgggtc cagagctcat
     3541 ggcggagttc catgaatgcc gccaccagtt cttgcttcga ttgaaaatgc cggtagagcg
     3601 tcatcttggc gacgccggaa tgctggatga tcgtgtcgat cccgaccgat cgcgtaccct
     3661 gctgtgaaaa caactgataa gcgctttcga ggatacggga gcgcgcatcc tcggcccgtg
     3721 ttcctgccgc gcgaaaccgt cctgcacggc tccaccgatc tgctcgccct cggcaaagcg
     3781 gtcatggcga acgacttgac cgcattggcg ctgctgagct gcgatgttgc gcgatctttg
     3841 ccggcgatca ccgccccgct ggatgcgatt ggcggcgatc cgatgcgccg cggtcggcgg
     3901 ggcctatctt caaaggctgg ggatcgaggg tctgagcgcg atcgcacggc tcggcccgtt
     3961 gcgcgatctc atgatgtccg cggccctcta tgcccgcagt cttccggccg tgaccgtggg
     4021 cactgaatta atcggagtcc tcgacctcga ttcgagcgcc atcgcccccg gccaaggaca
     4081 tcgatccggc tttgccaaga agctgaacgg tcggcatatg aacggaagac aactccccgg
     4141 acagcggcaa tcgcgaatac gttgcgccac ggattgtttt cacacttaga aatttaggaa
     4201 aaatctcaca taatgacttt atgcacatca ttgtggtgct aaatagattt taacaagcat
     4261 ttt
//
LOCUS       DASYEG010000190         2656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1073825, whole genome shotgun sequence.
ACCESSION   DASYEG010000190 DASYEG010000000
VERSION     DASYEG010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073825"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..802
                     /locus_tag="VGM72_08725"
     CDS             143..802
                     /locus_tag="VGM72_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265388.1"
                     /translation="MPPSLSSNFEENNRRLVAPVRTRLAAICAAAPLHARFLNMLSLL
                     EHIGSRKIMTSPAMQAPDLDALKHLAEEARHAVFFKRAAERTAARAFDYSPANTLAEP
                     SARLYMSRLDVEISRGVGAAAIPALPYLYMSLIVELRAVWLYRIYQAVLLEQKAGLTL
                     KSILAEEELHLCAMSARLSQLDRNAASRIPHFCAFEEVLFRHLWSEIEEACAVPSLAA
                     E"
     gene            809..1015
                     /locus_tag="VGM72_08730"
     CDS             809..1015
                     /locus_tag="VGM72_08730"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEY4265389.1"
                     /translation="MDIRRILLVSGAILLIAGLAWPIISRLGLGRLPGDIAIERQNFR
                     FYFPLATSLVVSVVLTLLFWLFRR"
     gene            1043..2626
                     /gene="purH"
                     /locus_tag="VGM72_08735"
     CDS             1043..2626
                     /gene="purH"
                     /locus_tag="VGM72_08735"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY4265390.1"
                     /translation="MIRRPVRRALLSVSDKTGLIDFARALAGHGVQLISTGGTAKILR
                     EAGLAVTDVSDITAFPEIMDGRVKTLHPKIHGGLLALRDKHADAMRTHGIEGIDLLVS
                     NLYPFESTIAKGAAFDEAIENIDVGGPAMIRAAAKNHEWVSVVVDPEDYATVLAEIAT
                     GGVSAETRLKLAGIAFARTAAYDSAVSNWLADMRAHQGEAEPPRRRAFAGILRRKLRY
                     GENPHQQAAFYATGEKHFGVATATQLQGKELSYNNINDTDAAYGLVAEFDTAIPACAI
                     IKHANPCGVALGSSLADAYRKALACDPVSAFGGVLAFNRPLDKATAEEIARLFTEVII
                     APDADQDARAILGAKKNLRLLIAGGLPDAHAPGWQFRSVAGGFLAQTRDALTAVPGDL
                     KVVTKRQPTDAESADMMFAFTVAKHVKSNTIVYAKDGATAGIGAGQMSRVDAARIAAA
                     KAREAAEAAGWPEPRTKGSAAASDAFFPFADGLLAVAEAGATAVIQPGGSVKDDAVIR
                     AADDAGLAMVFTGIRHFRH"
BASE COUNT          449 a          840 c          839 g          528 t
ORIGIN      
        1 tatttggccg cgcttggccc gcggctgaac gcgttctatc ttgagcgcga cgtgctcctt
       61 cgcaccctgg ggaatgtcgt ctacgttctt cctccctatt gcgtgcgagc aagtgagctc
      121 gacaggattt atgatgtcat cgatgcctcc ctcgctttcg tcgaattttg aggagaacaa
      181 tcgccgtctg gtggcgcccg tgcgcacgcg gctagccgcg atttgcgcgg ccgcgccgct
      241 ccatgcgcgc tttctcaaca tgctgtcgct gctggaacat atcggcagcc gcaaaatcat
      301 gaccagtccg gccatgcagg cgccggactt ggacgctttg aagcatctcg cggaggaggc
      361 gcggcatgcc gtcttcttca agcgcgccgc ggaacgcacc gccgcgcgcg cgttcgacta
      421 ttcgccggca aacacgctcg ctgagccttc cgcgcgactc tatatgagca gactcgatgt
      481 ggagatttcc cgcggcgtgg gcgcggcggc cataccagcg ctcccctatc tctatatgtc
      541 gctgatcgtg gagctgcgcg ccgtctggct ttatcgcatc taccaggccg tgcttttgga
      601 gcagaaggcc gggttgacgc tgaaaagcat tctcgccgag gaagagctgc atctctgtgc
      661 gatgtccgcg cgcctgtcgc aattggatcg gaacgccgcc agccggatcc cgcatttctg
      721 cgcattcgag gaggtcctgt tccggcattt gtggtccgag atcgaggagg catgcgccgt
      781 gccgagcctg gccgcggagt agttgcccgt ggatatccgc cgcattctcc ttgtttccgg
      841 cgccatcttg cttatcgcgg ggctggcttg gcccattatc agccggttgg gcctggggcg
      901 tctgcccggc gatatcgcga tagagcgcca gaatttccgg ttctactttc cgctcgccac
      961 gtctcttgtg gtgagcgtgg tcctcactct tctcttttgg ctgttccggc gttaagagcg
     1021 agcgaggttt ttcgatagtc ccatgatcag gcgtcccgtt cgccgtgcgc ttctttccgt
     1081 gtccgacaag acgggcctga tcgatttcgc gcgcgcgctc gcaggccatg gcgtgcagct
     1141 catctcgacg gggggaaccg ccaagatttt gcgcgaggcg gggctcgcgg tgacggatgt
     1201 gtcggacatc accgcatttc cggaaatcat ggatgggcgc gtaaagacgc tgcatcccaa
     1261 aatccatggc ggtctattgg ccctacgcga taagcatgcc gatgccatgc gcacgcatgg
     1321 gatcgaaggc atcgatcttc tggtttccaa tctctatccg ttcgaatcca cgatcgccaa
     1381 aggcgctgcg ttcgacgaag cgatcgagaa tatcgacgtc ggcggtccgg cgatgatccg
     1441 cgccgcggcc aagaaccatg aatgggttag cgtcgtcgtc gaccccgagg attatgcgac
     1501 cgtgctcgcc gaaatcgcca ccggcggggt gagcgcggag acgcggctga aactcgcggg
     1561 aatcgccttc gcccgcaccg ccgcctacga ttcggcagtg tcgaactggc ttgcggatat
     1621 gcgcgcccat cagggcgagg ctgaaccgcc gcgccgtcga gccttcgcag gaatattgcg
     1681 gcggaagctg cgctatggcg aaaatccgca tcagcaggcc gcgttctacg cgaccggcga
     1741 aaagcacttc ggcgtggcga ccgcgaccca gcttcagggc aaggaactct cctacaacaa
     1801 catcaacgac acggacgcgg cctatggact ggtggcggag ttcgacaccg ccattcctgc
     1861 ctgcgccatc atcaagcatg cgaacccttg tggcgtggca ctggggtctt cgcttgccga
     1921 tgcctatcga aaggctcttg cctgcgatcc cgtgagcgcc ttcggcggcg tcctcgcgtt
     1981 caaccggccg ctcgacaaag cgaccgccga ggaaatcgcc agactgttca ccgaggtcat
     2041 catcgcaccg gatgcggatc aggacgcacg ggccattctc ggcgccaaaa agaatctgcg
     2101 cctcttgatc gcaggcggtc tgccggatgc gcacgctccc ggttggcagt tccgctcggt
     2161 cgcgggcgga tttctggcgc agacacgcga tgcgctcacc gcggttccgg gagatttgaa
     2221 ggtggtcacc aagcgccagc ccaccgatgc cgagagcgcc gacatgatgt tcgcgttcac
     2281 agtggccaag cacgtcaagt ccaacaccat cgtctacgcc aaggatggcg caaccgcggg
     2341 catcggcgcc ggccagatga gccgcgtgga tgccgcgcgc attgccgcgg cgaaggcgcg
     2401 cgaagcggcc gaggccgcgg gctggcccga accgcgcacc aagggctcgg cggcggcgtc
     2461 cgacgcgttc tttcccttcg ccgatgggct tcttgcagtg gccgaggcag gtgcaacggc
     2521 ggtcatccag ccgggcggtt cggtgaagga cgatgcggtg atcagggcgg cggacgatgc
     2581 ggggcttgcg atggtcttca ccggcatccg ccacttccgg cactagattt ccgttgcgcg
     2641 ggtttcctgc acgaac
//
LOCUS       DASYEG010000191         7462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2091935, whole genome shotgun sequence.
ACCESSION   DASYEG010000191 DASYEG010000000
VERSION     DASYEG010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 7462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7462
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2091935"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /gene="hslU"
                     /locus_tag="VGM72_08740"
     CDS             <1..1000
                     /gene="hslU"
                     /locus_tag="VGM72_08740"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEY4265391.1"
                     /translation="LLEVGIGLVRSAKRKDVEAQAEAKAEERVLTALVGNAAAATRDS
                     FRKRLRAGELDDKEIEIEVRDAGGMPSLEIPGMPGAQIGMVNLGDIFGKALGGRTKTR
                     RLTVAASHVLLIAEEADKLLDNESILRDAIDNVENNGIVFLDEIDKICARGEIRGGAD
                     VSREGVQRDLLPLLEGTTVATKHGPVKTDHILFIASGAFHLAKPSDLLPELQGRLPIR
                     VELKALTREDFRRILTEPEASLIKQCVALLETENVMLDFTGDGIDAIAQIAADVNARV
                     ENIGARRLQTIMERVLDEISFTATDRNAETLTVDGAYVRARVEDLAKDQDLSRFIL"
     gene            complement(1007..1456)
                     /locus_tag="VGM72_08745"
     CDS             complement(1007..1456)
                     /locus_tag="VGM72_08745"
                     /inference="COORDINATES: protein motif:HMM:NF021495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HEY4265392.1"
                     /translation="MTDLWLAIIHHVLVFGLAIMLAVQASLLRDGMGAADAARIARLD
                     IGYGASAGLIVVIGVLRVIYGAKGYQYYVENVWFWAKMACFAAIGILSVPPTIRFRAW
                     HAAAKADADFAPPVAEIKKLRTYLRWEIRLLIPLVAFAATMARYTSF"
     gene            1593..2783
                     /gene="metK"
                     /locus_tag="VGM72_08750"
     CDS             1593..2783
                     /gene="metK"
                     /locus_tag="VGM72_08750"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018054574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY4265393.1"
                     /translation="MQRSNYLFTSESVSEGHPDKVSDRISDEVVDLFFREGPKEGMSP
                     WDIRAACETLCTTNRVVIAGETRGPKGVGAAQVEEVARAAIRDIGYEQDGFHWKRAQV
                     EVLLHAQSPHIAQGVDAAGNKDEGAGDQGIMFGYACRETPALMPAPLFYSHKILELLA
                     KVRKAGKEPKLGPDAKSQVTLRYANGVPVEATQIVLSTQHLDENMSSDDVREIVEPYI
                     RQTLPKEWITANTVWHVNPTGAFVVGGPDGDCGLTGRKIIVDTYGGAAPHGGGAFSGK
                     DSTKVDRSAAYAARYLAKNVVAAGVADRCTIQIAYAIGVADPLSLYVDLHGAGTADPA
                     KLEKILPELMSLKPRGIREHLGLNKPIYARTSAYGHFGREPDAEGGFSWEKTDLADKI
                     RAAL"
     gene            complement(2790..3560)
                     /locus_tag="VGM72_08755"
     CDS             complement(2790..3560)
                     /locus_tag="VGM72_08755"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY4265394.1"
                     /translation="MNEFALFAMVGLLAQLIDGGLGMGYGIVSATVLLALGSTPAAVS
                     ASTHAAELFTTAVSSGAHLLHRNVDFRIFWRLMPAGVIGGVIGAYILTGVNGEVTRPY
                     VVPYLGIMGVLILRRAFGRQKSKPISTRATGLYGIVGGFCDAVGGGGWGPVVTSAMLA
                     QGGEPRYVIGSVNASEFLVTMAISATFVVTLVSGHWERAGSLLDYATPVAGLVTGGII
                     AAPIASFLAKYLPARRLLAGVGLLVLALTTYQGWLLFA"
     gene            3692..4399
                     /locus_tag="VGM72_08760"
     CDS             3692..4399
                     /locus_tag="VGM72_08760"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF014447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(46)-N(7))-methyltransferase TrmB"
                     /protein_id="HEY4265395.1"
                     /translation="MTDNTHSRKLYGRRKGPALSAHQTGLLARLLPRLAFAPTAGRAA
                     DDYFSAPVQDVWLEIGFGAGEHLAWQAQSNPDIGIIGVEPYVAGMAKLLAKLAPLCGE
                     DGPDQRAVNIRLYNEDARDVIAALPDASLGRVFLLFPDPWPKTRHHKRRFVQMEMLDR
                     LARVMKPGAEFRFATDDPGYRVWALERLSAHPAFAWLAEDSIDWKTRPADWPQTRYEA
                     KAIHAGRACCYLRFARL"
     gene            complement(4400..5176)
                     /locus_tag="VGM72_08765"
     CDS             complement(4400..5176)
                     /locus_tag="VGM72_08765"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEY4265396.1"
                     /translation="MDLAPSLSVASQLEPPRAVGRRRWGPWATLGWAVPIMLVMVVFQ
                     TLGALAYLRLARFLHPGQTIALTSLGSNGAVLAFSLAVSAPAVLGIMALAVRLSRVPL
                     REYLALKWPRVRDVAIGVGSLAAVLLGTGLLASLTGQETPAFIADTFDSARAAGMLTL
                     LVVTFVFLGPLQEELLFRGFLFRGFSLSFGTWPTIVLTAAAWAIIHVQYQWFFIGEIF
                     ALGLLFGWLRARSGSLLLTFGLHAAVNAMAVTSAALMPGA"
     gene            5412..5948
                     /gene="rimP"
                     /locus_tag="VGM72_08770"
     CDS             5412..5948
                     /gene="rimP"
                     /locus_tag="VGM72_08770"
                     /inference="COORDINATES: protein motif:HMM:NF000932.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEY4265397.1"
                     /translation="MNEVVLSAENPLDVLLAPAVEAAGYRLVRLRVMGAKKKTLQVMA
                     ERPDGSMNVEDCALLSRTLSELLEAADPIAEEFVLEVSSPGIDRPLTSETDFVRFAGH
                     DVRMEMTHGVNGRRRFKGVLAGLDGHEVLMDVTGEDGPRRVRLPFGDIADAKLVLTDR
                     LIQESLKARESREVQSSH"
     gene            6003..>7462
                     /gene="nusA"
                     /locus_tag="VGM72_08775"
     CDS             6003..>7462
                     /gene="nusA"
                     /locus_tag="VGM72_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY4265398.1"
                     /translation="MAIGIAANRLELLQIADAVAREKSIDKSVVIHAMEEAMQRAAKS
                     RYGTENEIRVEIDPKTGETHISRYLHVVDQIENDKIEISMADARRRNPAAQVGDIIAE
                     TLPPIEFSRIATQAAKQVIVQKVRDAERERQYDEYKDRIGEIVHGTVKRVEFGSVVVD
                     FGRAEGVVRRDEMIPRESFRNGDRIRAYIYDVRRETRGPQIFLSRTHPQFMARLFAQE
                     VPEIYDGIIEIRAVARDPGSRAKIAVISKDSSIDPVGACVGMRGVRVQAVVQELQGER
                     IDIIPWNGDPATFIVNALAPAEVSKVVLDEETRRVEVVVPDDQLSLAIGRRGQNVRLA
                     SQLTGWQIDILTEQEESERRQKEFAERTALFMEALDVDETVAQLLASEGFASVEDVAY
                     VPVHELAEIEGFDEETAEELQARATGHIDQKNAAFDAKRRELGVADEIAETEGLTPPM
                     LVKLGENGIKTLEDLAGCASDDLLGYVENKGNERVRV"
BASE COUNT         1551 a         2300 c         2374 g         1237 t
ORIGIN      
        1 ccttctggaa gtgggcatcg gtctggtgcg ttcggccaag cgcaaggacg tcgaggccca
       61 ggccgaagcc aaggccgagg aacgcgtgct gacggcgctg gtgggaaacg cggccgccgc
      121 gacacgcgat tccttccgca agcgcctgcg tgcgggcgaa ctcgacgaca aggagatcga
      181 gatcgaggtg cgcgatgccg gcgggatgcc gtcgctcgaa attcccggca tgccgggcgc
      241 ccagatcggc atggtgaatc tgggcgatat cttcggcaag gcgctgggag ggcgcaccaa
      301 gacgcggcgc ctgaccgtcg cggcctcgca tgtccttctg atcgccgaag aggccgacaa
      361 gctgctcgac aatgaatcca tcttgcgcga tgccatcgac aatgtggaaa acaacggcat
      421 cgtgttcctg gacgagatcg acaagatctg cgcccgcggc gaaatccgcg gcggtgccga
      481 tgtcagccgc gaaggcgtgc agcgcgatct tctcccgctt ctcgaaggga cgacggtcgc
      541 caccaagcac ggaccggtga agaccgatca catcctgttc attgcgtccg gcgcgttcca
      601 tctggccaag ccgtccgacc tcttgccgga gttacaaggg cgccttccca tccgggtgga
      661 gctgaaggcg ctcacgcgcg aggatttccg gcgcatcctg accgaacccg aagccagcct
      721 catcaagcaa tgtgtggcgc tcttggagac ggagaacgtc atgctggatt tcaccgggga
      781 tgggatcgac gccatcgcgc agatcgccgc cgacgtgaac gcgcgggtgg aaaatatcgg
      841 cgcgcggcgg ctgcagacca tcatggaacg ggtgctcgac gagatcagct tcaccgccac
      901 cgaccgcaac gccgaaacgc tcaccgtcga cggggcctat gtgcgcgcgc gggtggagga
      961 cctcgccaag gatcaggatt tgtcgcgttt cattctgtga gcgacgctag aagctggtat
     1021 agcgcgccat ggtcgcggcg aacgccacca gcggaatcaa cagccggatt tcccatctga
     1081 gataggtgcg aagctttttg atctcggcaa cgggaggcgc gaaatccgca tcggccttgg
     1141 ccgccgcgtg ccaggcccgg aaacggatgg tgggcggcac ggacaaaatc ccgattgcgg
     1201 cgaagcacgc catcttggcc cagaaccaga cattctcgac ataatattga tagcccttgg
     1261 cgccgtagat cacgcgcaaa acgccgatca cgacgatcaa tcccgccgag gcgccataac
     1321 cgatatcgag acgcgcgatc cgtgcggcat ccgccgctcc catgccgtcg cgcagcagcg
     1381 aggcctgtac ggccagcatg atcgccaagc cgaacaccag cacatgatgg atgatcgcca
     1441 accaaagatc ggtcatgcgg ccgctttgct ccaccaggat tctgtcgtgg aatttaccaa
     1501 tagttttcgt gggataccag gaacgatcta gcgcattgac cccttgggga ttccctgcaa
     1561 gaaacgccct cctggcgcac acgagggcgg agatgcagcg ttcaaactat ctcttcacga
     1621 gcgaatcggt ttccgaaggc catcccgaca aagtaagcga ccgcatctcg gatgaggtcg
     1681 tggatttgtt tttccgcgaa ggccccaagg aaggcatgag cccctgggat attcgcgccg
     1741 cctgcgaaac cctctgcacc accaaccgtg tcgttatcgc gggcgagacc cgtggtccca
     1801 agggcgtcgg tgcggcacag gtggaagagg tagcgcgcgc cgcgattcgt gacatcggtt
     1861 atgagcagga cggcttccac tggaagagag cccaggtcga agttctgctg cacgcgcaat
     1921 cgccgcacat cgcgcaaggc gtggatgcgg cgggcaacaa ggatgagggt gcgggcgatc
     1981 agggcatcat gttcggctat gcctgccgcg agacccccgc gctgatgccg gcgccgctgt
     2041 tctacagcca caaaatcctg gaattgctcg ccaaagtccg caaagcgggc aaggagccga
     2101 agctcggtcc ggatgccaaa tcgcaagtca cgctgcgcta tgcgaatggc gttccggtgg
     2161 aagccaccca gatcgtgctc tcgacccagc atctcgacga gaacatgtcc tcggacgatg
     2221 ttcgcgagat tgtcgagccc tatatccgcc agaccctgcc gaaggagtgg atcaccgcca
     2281 acaccgtctg gcacgtcaat cccacggggg ccttcgtggt cggcgggccg gatggcgatt
     2341 gcggcctcac cggacgcaag atcatcgtcg acacctacgg cggggctgct ccccatggcg
     2401 gcggcgcctt ctcgggcaag gattcgacca aggtggaccg ctcggccgcc tacgccgcgc
     2461 gctatctcgc caagaatgtg gtggcggctg gcgtcgcgga tcgctgcacc atccagatcg
     2521 cctatgccat cggcgtggcg gaccccctct cgctttatgt cgacctgcac ggcgcaggga
     2581 ctgcggaccc ggcgaagctg gagaaaatcc tgcccgagct gatgagcctg aaaccgcgcg
     2641 gcatccgcga acatctgggc ctgaacaagc cgatctatgc ccgcacctct gcttacggcc
     2701 atttcgggcg cgagcccgat gccgaaggcg ggttctcctg ggagaagacc gatctcgcgg
     2761 acaagatcag agccgccctc tgattgccgt caggcgaaca gcagccagcc ctgataggtg
     2821 gtcagcgcca gcacgagcag accaacgccc gcgagcaatc tgcgcgcggg tagatatttg
     2881 gcaagaaagc tcgcaatcgg agctgcgatg atgcctccgg tcaccaagcc ggcgaccgga
     2941 gttgcataat cgagaagact ccccgccctc tcccaatggc cggaaacgag cgtcacgacg
     3001 aatgtggcgg aaatcgccat cgtgactagg aattccgagg catttaccga gccgatgacg
     3061 tagcgaggct cgccgccttg ggccagcatc gcgctcgtga ccaccgggcc ccagccgccg
     3121 ccgcccacgg catcgcaaaa gccgcccacg atcccgtaaa gtcccgtggc gcgcgtcgaa
     3181 atgggtttgg atttttggcg cccgaaggcg cgcctcagaa tgagcacgcc catgatgccg
     3241 agatacggca cgacataggg acgcgtgacc tcgccattca cgccggtcag gatgtaggcc
     3301 ccgatgacgc cgccgatgac acctgccggc atcagacgcc agaagatccg gaagtctacg
     3361 ttgcgatgca aaagatgcgc cccgctcgaa accgcggtgg tgaagagttc ggccgcatgg
     3421 gtactggcgg aaacggcggc cggcgtggat cccagggcca gaagcaccgt cgccgagacg
     3481 atgccgtagc ccattccaag cccgccatcg atgagttggg cgagaagccc caccatggcg
     3541 aaaagagcga attcgttcat ccaaaccgcg ttgccaatat cctggtcatc gtcaaatcct
     3601 ggttccttcc gtctcccgat agaacgcgaa gcggtgtgtt aaggcgtcag aacaggttga
     3661 ccctggctgt ttccaggcta tcgcttccgg gatgaccgac aacacacatt cccgaaagct
     3721 ttatggccgc cgcaaaggcc cggctttaag cgcgcatcag acggggctgc tcgcccggct
     3781 gctccctcgg cttgcattcg ctcccacggc cggccgcgcg gcggacgatt atttttctgc
     3841 gcccgttcag gacgtctggc tggaaatcgg gttcggcgcc ggcgagcatc tggcttggca
     3901 ggcgcaaagc aatccggaca tcggcatcat cggcgtcgag ccctatgtcg cgggcatggc
     3961 gaaactcctg gcgaagctcg cgcctctgtg cggggaagac ggcccggatc aaagggcggt
     4021 gaacatccgc ctctataacg aagatgcgcg cgatgtgatc gcggccctcc ccgatgcgag
     4081 ccttgggcgc gtgttccttc ttttccccga tccctggccg aagacgcgcc accacaagcg
     4141 gcgatttgtc cagatggaga tgctggatcg tctcgcgcgc gtgatgaaac ccggcgccga
     4201 atttcgcttc gcgaccgacg atcccggcta tcgggtctgg gcgctggagc gtctgtcggc
     4261 gcatcccgcg ttcgcatggc tcgcggaaga ttccatcgat tggaagacgc gcccggcgga
     4321 ttggccgcaa acccgctacg aagcgaaagc gatccatgcg ggacgcgcgt gctgctatct
     4381 gcgttttgcg cggctttaat tacgcaccgg gcatcagggc cgcgctcgtc acggccatgg
     4441 cattgacggc cgcgtgtaac ccgaatgtca ggagcaggga cccgctgcgc gccctcagcc
     4501 agccgaacag caggcccagc gcgaaaatct cgccgatgaa gaaccactga tattgcacat
     4561 ggataatcgc ccatgccgca gcggtcagca cgattgtcgg ccacgtgccg aaggacagcg
     4621 agaacccgcg gaacaggaaa ccgcgaaaca gcagttcctc ttgcaggggc ccgaggaaga
     4681 cgaaggtcac gaccagtagc gtcagcatgc cggcggcccg cgcgctgtcg aacgtgtcgg
     4741 caatgaaggc cggagtttcc tgtcccgtga gcgaagcgag aagcccggta cccaggagca
     4801 cggcggcaag cgaacccacg ccaatggcga cgtcgcgcac tcgcggccat ttcagcgcca
     4861 gatattcgcg taaagggacg cgggacagcc tgaccgcgag cgccatgatg cccagcacgg
     4921 ccggcgcgga aaccgccagc gagaacgcca atacggcgcc attggagccc agcgatgtga
     4981 gcgcaatcgt ttgtccggga tgaaggaacc gcgccagtcg cagataggcg agcgcgccca
     5041 gggtctggaa cacgaccatc accagcatga tgggaacggc ccagccgagt gtcgcccacg
     5101 gaccccagcg gcgccgtcca accgcgcgcg gcggttcgag ctgcgaggcg acggaaagag
     5161 aaggagccag atccatgaga atgccttaaa ggaacaaatt taccgcgtct gtcacgattt
     5221 catgatggaa aacgcgccag atggtgaacg ggttatagaa gcgatattcc gcggaccatc
     5281 ttgtaatgac aggagggaca gcgtagatag gcgtcattcc tgacaacaga aaactgttgt
     5341 gtggcgattt aagagatcgc cgcggccgga aagctttggt ttgggaaagc tttggtttgg
     5401 gaagcgatgt tttgaatgag gtcgtcctga gcgctgagaa tcccctggat gtgctgcttg
     5461 cccccgcggt cgaggccgcg ggctaccggc tggtgcgtct gcgcgtgatg ggcgcgaaaa
     5521 agaaaacgct gcaagtgatg gccgaacgtc ccgacggcag catgaatgtc gaggattgcg
     5581 cgttgctgtc gcgcacgctt tccgagctgc tggaagcggc cgacccgatt gccgaggaat
     5641 tcgtgctgga agtgtcgtcg ccgggcatcg accgcccgct gacgtcggaa accgatttcg
     5701 tccgttttgc cggccatgat gtgagaatgg aaatgaccca tggggtgaac ggcaggcggc
     5761 gcttcaaggg cgtgctcgcc ggtctcgatg ggcacgaggt gctgatggat gtgacgggcg
     5821 aggacgggcc gcggcgcgtg cgtctgccct tcggcgacat cgcggatgcg aagctcgtcc
     5881 tgacggaccg gctgatccag gaaagtttga aggcaaggga atcgcgcgaa gtccaatcgt
     5941 ctcattaggg caatcttccc gccgggacgg gcgtcgcatt cattcaatcg ggagtgaaca
     6001 tcatggctat cggaattgcc gccaaccggc tcgaattgct gcagatcgca gacgccgtcg
     6061 cgcgcgagaa atcgatcgac aaatcggtcg tcatccatgc gatggaagaa gcgatgcagc
     6121 gcgccgcgaa atcgcgttac ggcaccgaga acgagatcag ggtcgaaatc gatcccaaga
     6181 ccggcgagac acatatctcg cgatatctgc acgtcgtgga tcagatcgaa aacgacaaga
     6241 tcgagatttc catggccgat gcgcgccggc gcaaccccgc cgcccaggtc ggcgacatca
     6301 tcgccgagac gctgccgccc atagaattct cacgcatcgc aacccaggcc gccaagcagg
     6361 tgatcgtgca aaaggtgcgc gacgccgagc gcgaacgtca atatgacgaa tacaaggacc
     6421 gcatcggcga gatcgttcac ggcacggtca agcgcgtcga attcggctcc gtggtggtcg
     6481 atttcgggcg cgccgagggc gtggtccgcc gcgatgagat gatcccgcgc gaatccttcc
     6541 gcaatggcga ccgcatccgc gcctatatct acgatgtgcg ccgcgaaaca cgcggcccgc
     6601 agattttcct ctcccgcacc catccccaat tcatggcgcg cttgttcgcg caggaagtgc
     6661 cggaaatcta tgacggcatc atcgaaatcc gcgcggtggc gcgcgatccg ggaagccgcg
     6721 ccaagatcgc ggtgatctcc aaggattcct cgatcgatcc ggtgggcgcg tgcgtcggca
     6781 tgcgcggcgt gcgcgtgcag gcggtcgtcc aggaattgca gggcgaacgc atcgacatca
     6841 ttccgtggaa cggcgatccg gccactttta tcgtgaatgc gctggctccc gccgaagtct
     6901 ccaaggtggt gctggatgag gaaacgcgcc gcgtcgaagt ggtggtgccc gacgatcagc
     6961 tttcgctcgc catcggccgg cgcggccaga atgtacggct ggcgtcccaa ttgaccggct
     7021 ggcaaatcga cattctgacc gagcaggaag aatccgagcg ccgccaaaag gaattcgccg
     7081 agcgcacggc gctcttcatg gaagccctgg atgtggatga gaccgtcgcc cagctcctgg
     7141 catcggaagg gttcgcgagc gtggaggacg tcgcctatgt gcctgtccat gaactcgccg
     7201 agatcgaagg cttcgacgaa gagaccgccg aagagctgca ggcgcgcgcg accggccaca
     7261 tcgatcagaa gaacgccgcg ttcgacgcca agcgccgcga acttggcgtc gcggacgaaa
     7321 ttgccgagac cgaagggctg acgcccccca tgctggtgaa gctcggcgag aacgggatca
     7381 agacgctcga agatctggcc ggctgcgcca gcgacgacct tcttggttat gtcgagaaca
     7441 agggcaatga acgcgtgcgc gt
//
LOCUS       DASYEG010000192         6544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2233860, whole genome shotgun sequence.
ACCESSION   DASYEG010000192 DASYEG010000000
VERSION     DASYEG010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6544
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2233860"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1446)
                     /locus_tag="VGM72_08780"
     CDS             complement(13..1446)
                     /locus_tag="VGM72_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY4265399.1"
                     /translation="MNMMMKWFSRTVVAGAAAFLIMGGAVHAQPAPADAPAAEAAPAA
                     PAAAPDAAAPAAAPAAATPAPPKIDSGDTAWMITSSALVLMMTIPGLALFYGGMVRKK
                     NVLATLAQSFGATCIITVLWMVIGYSLAFTTNPDAGVNSFIGGVNYLLLGPMGVNATT
                     MLAPTIPESVYMFFQMTFAIITPALIAGALADRMKFSAFMWFMSLWLLLVYCPIAHWV
                     WGGGWLGTLGALDYAGGTVVHLNAGTAGLITCLVLGKRVGLGTENMSPHNLVLSVIGA
                     SLLWVGWFGFNAGSAVTANVNAGMAAAATQIATAAAAMAWMAVEWMVAKKPSVLGMIS
                     GAVAGLVAITPASGFVDPTGALIIGFIAGFVCYLSSVHMKKIFGYDDALDAFGVHGVG
                     GALGAMLTGVFASSAINMAGKGWIHDGNGHQMLIQFYDVAGVFVYCAIATYVILKIID
                     MIVGLRVKRDVEVEGLDINLHGETVHA"
     gene            complement(1477..1815)
                     /locus_tag="VGM72_08785"
     CDS             complement(1477..1815)
                     /locus_tag="VGM72_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY4265400.1"
                     /translation="MKFITAIIKPFKLDEVREALSALGVQGMTVSEVKGYGRQKGHTE
                     IYRGAEYAVNFLPKIKIEVAVRSEMADAVIEAITNNARTGQIGDGKIFVTSVDQVVRI
                     RTGEIDNDAL"
     gene            2147..3325
                     /locus_tag="VGM72_08790"
     CDS             2147..3325
                     /locus_tag="VGM72_08790"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEY4265401.1"
                     /translation="MSETIPATVSTSPGSAVAAPWAWASFKRALRTLHLWLGIALCLP
                     MIAIGLSGSALLAQRQILWLWVPAVSSAGERQSLSRIVAAAEAAMPVKANWVEIPRSQ
                     RQPAAVQFIVSNRPNRTVEVLVDPISLKLLGSSELVRRGPIMAFMVRIHEFLMLPDRI
                     GLPAVGWTAIAMTVMGLSGLVLWWPRKGRWAAAFLVKRGARGLRLHLDLHHAIGIWGL
                     LIFLVLSVSGIYLAFPQTVSGAVQAAFPARETEAKPMPPGFPETWPIPPDEAVAIAKA
                     AVPDAIPVGILLPRNPGAVLMVQMETHAIAPSIPPITVAFDPSNTGTIQIDDPRNYPL
                     PDRILNLLYALHFSVGVGGLWTFLVFLAGLLPLFLAVTGVTIWWTKRARRRAAALLRA
                     "
     gene            3340..4566
                     /locus_tag="VGM72_08795"
     CDS             3340..4566
                     /locus_tag="VGM72_08795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY4265402.1"
                     /translation="MDMMQKDVVIAGGGMAGLTLALALAQGGLEVALADSLTRAQMED
                     ERFDGRVAALAFASVRMLIVLGVWEQLEKDAQPIADILVNEGTLGHGPTPFSLHFDHR
                     EIGEPLGHIVENRHIRQALLAAVAADKRIALFPSASATGLTIGEERAVLSLSTGETIA
                     ARLAVAAEGRDSALREAQGIGTVGWDYGQTGIVTTVAHERPHKGVAYEQFLPTGPFAI
                     LPMTGNRASLVWTERTAEALRIVALPEAAFTAELIARFGNHLGAVVPVGPRWSYPLKL
                     HLARSYVKDRFALAGDAAHTIHPLAGQGLNLGLKDVAALAETVLDAARLGLDYGRLDV
                     LKRYERWRRFDSFALAAVTDSLNRLFSNDFLPLRWARDLGLGAVDRIPPLRRFFMRHA
                     GGDLGTLPRLLKGEAA"
     gene            4799..6481
                     /locus_tag="VGM72_08800"
     CDS             4799..6481
                     /locus_tag="VGM72_08800"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY4265403.1"
                     /translation="MALRDITRNLALGTAAAAVMLCAQPVFALDNAGSLEGVVKNAAG
                     QPVSGAFVRLKNADKRLTFMVVSQNQGAFSAKDLPPGRYTVQGISGASQSAISAPVDV
                     ASDKASKIDVALSGARGPLLTPAWPGRVPEAQVTKISTDAKDLPAGDGKALMSEKCTV
                     CHDVQRILSKRSDLSDWTFTVKRMRVNMAAASLPDISDADAAAIVKYASANFKPLPGG
                     DDINGRLPTYAMTGESTKYRVVTLDLPRRYSEPHDIASDPSGVGWAAERAGALIRFDP
                     KTYAFTEVKVPAGPAPADRQRLGNPQINAQGILWTADGPNSRWLSYDTKAFSAANPQV
                     PFHIYPWTLKGHGGAGGNSFALAPDGTVWGTGQGREVRMLNPATAEFKTFESPSYKQT
                     KESPGAYGLAVAGDGSIWWAEDDADMMARADPKTGEVTEYKLPNVGGHALPRRMNSDW
                     DGNLWVGVWEAGKLMKVDYKTRQMTVYTPTTPSLGMYSVVADKKNHLIWVSAQQVDKI
                     FRFDPATAKFTEYPLSYAQQDPRRIDIDPTNSNRIFFSGNTADRIGFIELLP"
BASE COUNT         1166 a         2060 c         2066 g         1252 t
ORIGIN      
        1 gcacgttgcc gatcaggcgt ggacggtctc gccgtggagg ttgatgtcga gcccttcgac
       61 ttcgacatcg cgcttcacgc gcaggcccac gatcatgtcg atgatcttga ggatgacgta
      121 ggtggcgatg gcgcagtaca cgaagacgcc ggccacatcg tagaactgaa tcagcatctg
      181 atggccgttg ccgtcgtgga tccaaccctt ccccgccatg ttgatcgcgc tggaggcgaa
      241 cacacccgtc agcatcgcgc cgagcgcgcc gccgacgcca tgcacgccga aggcatcgag
      301 ggcgtcgtca tagccgaaga tcttcttcat gtgcaccgaa gagagatagc agacgaagcc
      361 ggcgataaag ccgatgatca gcgcgcccgt tggatcgacg aagcccgaag ccggcgtgat
      421 cgcaaccagg cccgccaccg cgcccgagat catgccgagc acggacggct tcttggcgac
      481 catccactcc accgccatcc aggccatggc ggcagccgct gtcgcgatct gggttgccgc
      541 cgccgccatg ccggcgttga cgttggcggt caccgcgctg cctgcgttga acccgaacca
      601 gcccacccac agaagcgagg ccccgatgac gctgagcacg agattgtgcg gcgacatgtt
      661 ctcggtgccg aggcctacgc gttttcccag cacgaggcag gtgatgaggc ccgccgtgcc
      721 cgcgttcagg tgcaccacgg tgccgccggc atagtcgagg gcgcccaacg tgcccagcca
      781 gccgccgccc cagacccaat gcgcgatcgg gcaatagacc agaagcagcc agagcgacat
      841 gaaccacatg aaagcggaaa acttcatgcg gtccgccagc gcgccggcga tcagggcggg
      901 cgtgatgatg gcgaatgtca tctggaagaa catgtagacg gattcaggaa tggtcggcgc
      961 cagcatcgtg gttgcattca cgcccatagg gccgagcagc agataattga cgccccctat
     1021 gaaggaattg acccccgcgt cggggtttgt cgtgaaggcc agggaatagc cgatgaccat
     1081 ccacagcacg gtgatgatgc aagtggcgcc gaaactctgc gccagcgtcg cgagcacgtt
     1141 cttcttgcgc accatgccgc cataaaaaag ggccagcccc ggaatggtca tcatcagcac
     1201 gagagcgctc gatgtgatca tccaggcggt gtcgccggaa tcgatcttgg gaggtgcggg
     1261 cgttgcggcc gcaggcgcgg cggcgggcgc ggccgcgtcg ggtgcggctg ccggagcggc
     1321 aggcgcggct tctgccgcag gcgcgtccgc gggtgcgggt tgcgcatgga cggcgccgcc
     1381 catgatcaaa aaggccgcgg cgccagccac cacggtcctg gaaaaccatt tcatcatcat
     1441 gttcatcgtt taaatcccct caaatcctat atttgctcag agtgcgtcat tgtcgatctc
     1501 gcccgtccgt atacggacga cctgatccac cgatgtgacg aaaatcttgc cgtccccgat
     1561 ctgcccggtc ctggcattgt tggtgatggc ttcgatcacc gcgtccgcca tttcgctgcg
     1621 cacggcgact tcgatcttga ttttgggcag gaagttcacc gcgtattccg cgccgcgata
     1681 gatttccgta tgtcccttct ggcgcccata gcccttgacc tcgctgacgg tcatgccctg
     1741 gacgcccagc gccgacagcg cttcgcgcac ttcatcgagc ttgaacggct tgatgatggc
     1801 cgtgatgaat ttcatgctgg tgcccctgtt gaactgattt ttccgctggc cggtttccgc
     1861 ggccgcctta tcggatatca agaagcgtgc cggttttgcg cgatgcggta agtccctgat
     1921 gttactgggt agaatttctg attatgacgg gaatgtcaga ttggtcgccg accgggtgat
     1981 taatttttgt gcaaaaaaag aaaattgcac aatatttgtg cggctgatcg aaataagcgg
     2041 ctgcggcgcc cagcggcatg gcctcgcgtg cgtttgccct tgcgggaaac accaagatgg
     2101 ctcacccagt caaaagaccc gaaaagtggc ggcaccgcga ctcgttatga gcgaaacgat
     2161 tccagcgacg gtctccacct ctcccgggag cgccgttgcc gcgccttggg cgtgggcatc
     2221 gttcaagcgc gcgctgcgca cgctgcatct ttggctcggg atcgcgttgt gcctgccgat
     2281 gatcgcgatc ggcctgtcgg gcagcgcgct gctcgcgcag cggcagatcc tgtggctttg
     2341 ggtgccggcg gtttcctcgg caggcgagcg tcaatcgctg tcgcggattg tggcggcggc
     2401 agaggccgcc atgccggtga aagcgaactg ggtggagatt ccgcgctcgc aaaggcagcc
     2461 ggctgccgtc caattcatcg tgtcgaaccg tcccaaccgc accgtcgagg ttctggtcga
     2521 tccgatttcg ctgaagctgc ttggctcgtc cgagctggtg cggcgcgggc ccatcatggc
     2581 cttcatggtc aggatccatg agttcctaat gctgccggac cgtatcggtc ttcccgccgt
     2641 cggctggacg gccattgcca tgacggtcat gggtctctcc gggctcgtat tgtggtggcc
     2701 gcgaaagggc cgctgggccg ccgcgttcct ggtgaagcgc ggcgcgcggg gattgcggct
     2761 ccatctcgat ctgcatcatg ccatcgggat ttggggactt ctgatctttc tcgtgctcag
     2821 cgtcagcgga atctatctgg cgttcccgca gacggtcagc ggtgccgtcc aggcggcctt
     2881 tcccgcgcgc gaaaccgagg caaaaccgat gccgccgggc ttcccggaga cctggccgat
     2941 tccgcccgat gaggccgtcg ccatcgcgaa agccgcggtg cccgacgcga tccctgtcgg
     3001 tatcctgctt ccccgcaacc ccggcgccgt gctgatggtg cagatggaga cccacgcaat
     3061 cgcgccttcc attcctccga tcacggtggc tttcgacccc agcaacaccg gcacgatcca
     3121 gatcgacgat ccgcgaaact atccccttcc ggaccgcatc ctgaacctgc tctatgcgct
     3181 gcatttcagc gtcggcgtcg gcgggctctg gacgttcctc gttttccttg ccgggctgct
     3241 gccgctgttc ctggccgtca ccggagtgac catctggtgg acgaagcgcg cacgccggcg
     3301 cgcggcggct ttgttgcgcg cctgagcgga cgacaaacaa tggacatgat gcaaaaggat
     3361 gtcgtcattg cgggtggggg gatggccggg ctcacccttg cgctcgcgct cgcgcaaggc
     3421 ggtctcgagg tcgcgctcgc cgattccctg acccgcgcgc agatggaaga cgagcgcttc
     3481 gacggacggg tggccgcgct cgcctttgcg tccgtccgta tgctgatcgt attgggcgta
     3541 tgggagcagc ttgaaaaaga cgcccagccc atcgccgata ttctggtgaa cgagggaacg
     3601 ctgggccatg gcccgacgcc gttctcgttg catttcgatc atcgcgagat cggcgagccg
     3661 ctcggtcata tcgtcgagaa tcgccatatc cgccaagcgc ttctcgccgc tgttgcggcg
     3721 gacaagcgga tcgccctgtt cccgagtgcg tccgccaccg gcctcacgat cggtgaggaa
     3781 cgcgcggtcc tgagcctttc caccggcgag accatcgccg cgcgcctggc ggttgcggcg
     3841 gaggggcgcg attccgcctt gcgcgaagcg caggggatcg ggaccgtcgg ctgggattac
     3901 ggacagaccg gcatcgtgac gacggttgcc catgagcgcc ctcataaggg cgtcgcctat
     3961 gagcaatttc ttccgaccgg gccgttcgcg atccttccca tgaccggcaa ccgtgcttct
     4021 ttggtgtgga cggaacgaac cgccgaagcg ctgcgcattg tggcgctgcc cgaggctgcg
     4081 ttcaccgccg aactgatcgc gcgcttcggg aatcatctgg gcgcggtcgt tcccgtgggt
     4141 ccgcgctggt cctatccgtt gaagctccat ctggcgcgaa gctacgtgaa ggatcgcttc
     4201 gcgctcgcgg gcgatgccgc ccacaccatc cacccgctgg cgggacaggg cttgaatctg
     4261 ggcctcaagg atgtggcggc gctggccgag accgtgctgg atgccgcgcg gctggggctc
     4321 gactatggcc gtctggacgt gctcaagcgc tatgagcgct ggcggcgttt cgacagtttc
     4381 gcgctcgcgg cggtgaccga ttccctgaat cgcctgtttt ccaatgattt cctgccttta
     4441 cgctgggccc gcgatctcgg actgggagcg gtggaccgca tcccgccctt gcggcgattc
     4501 ttcatgcgcc atgccggggg cgacctcggg acattgccgc gccttctcaa aggcgaggcg
     4561 gcctagccgg atttttcgga cggggacgac cgagcggccg atgaccccca tgaatccgat
     4621 cctccttctc acgaaaatgt gctagtcatc gttccagtca tcgcgacggc tgcatgtgtc
     4681 agtgcggccg ctgcaattgt tcctcgcttc acgtggtcac gttcaaacgc ggtttgcatt
     4741 gaaacgatca ccttgcccgg tacgccaatc gtacaaggtg atgagaggag gacagcaaat
     4801 ggcactcagg gacatcacgc gcaatcttgc tttgggaaca gcggcagcgg cggtcatgct
     4861 ctgcgcgcaa ccggttttcg cgctcgacaa tgcgggttca ctggaaggcg ttgtgaagaa
     4921 cgccgccggg cagcccgtga gcggcgcgtt cgtgcggctg aagaacgccg acaaacgcct
     4981 gaccttcatg gtggtcagtc agaatcaggg cgcgttcagc gccaaggatt tgccgcccgg
     5041 cagatatacc gtccagggaa tcagcggcgc cagtcagagc gctatttcgg cgccggtcga
     5101 tgttgcctcc gataaggcct ccaagatcga cgtcgcattg tccggtgcgc gcggaccgct
     5161 gttgacgccg gcatggccgg gccgcgtccc ggaagcgcag gtgaccaaga tttccaccga
     5221 tgcgaaggac ctgcccgccg gtgacggcaa agctctgatg agcgagaagt gcaccgtctg
     5281 ccacgatgtg cagcgcatcc tgtccaagcg ttcggatctc agcgactgga cattcaccgt
     5341 gaagcggatg cgggtgaaca tggcggctgc gagtttgccg gatatttccg atgccgatgc
     5401 cgcggcgatc gtgaaatatg cgtcggccaa tttcaaaccg ctgcccggcg gcgacgacat
     5461 caatggccgc ttgccgactt atgcgatgac cggcgagagc acgaaatatc gcgtcgtcac
     5521 gctcgatctt ccccggcgct attccgagcc tcatgacatc gcctccgatc cgtcgggcgt
     5581 gggctgggcg gccgagcgcg cgggcgcttt gatccgcttt gatcccaaga cctatgcgtt
     5641 cacggaagtg aaggtccctg ctggaccggc tccggcggac cgtcaacgcc tcggcaatcc
     5701 gcagatcaat gcccagggca tcctgtggac ggccgacggc cccaacagcc gctggctcag
     5761 ctacgatacc aaggccttca gcgcggctaa tccgcaggtg ccgttccata tctatccgtg
     5821 gacgcttaaa ggtcacggtg gcgcaggcgg gaattccttc gcgctcgctc cggacggaac
     5881 ggtgtgggga acgggccagg gacgcgaggt gcgcatgttg aatccggcga cggccgagtt
     5941 caagaccttc gaatccccga gctacaagca gaccaaggag agcccgggcg cgtatggcct
     6001 tgcggtggcc ggcgacggct ccatctggtg ggccgaagac gatgccgaca tgatggcgcg
     6061 tgccgatccc aagaccggcg aagtcacgga gtacaaactc ccgaacgtgg gcggccatgc
     6121 gcttccccgc cgcatgaaca gcgactggga cggcaatctg tgggtcggag tctgggaggc
     6181 cggcaagctg atgaaggtcg actacaagac caggcagatg acggtctaca caccgacgac
     6241 tcccagcctc ggcatgtatt ctgtcgtggc cgacaagaag aaccatctca tctgggtcag
     6301 cgcccaacag gtcgataaga ttttccgctt cgaccccgca acggccaagt tcaccgaata
     6361 tccgctgtcc tatgcgcaac aggacccgcg ccggatcgac atcgatccga ccaattcgaa
     6421 ccgcatattc ttcagcggca atacggcgga tcgtatcggc ttcatcgaac ttctgccgta
     6481 agccgcagcg gctttgtttt gcttttcggg ccaggtcctc acggacctgg ccctttttat
     6541 ttgg
//
LOCUS       DASYEG010000193         1663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1074400, whole genome shotgun sequence.
ACCESSION   DASYEG010000193 DASYEG010000000
VERSION     DASYEG010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074400"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VGM72_08805"
     CDS             <1..654
                     /locus_tag="VGM72_08805"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4265404.1"
                     /translation="TLKSKDGQSETLTVDRVILAVGIVGNVENIGLEQVGIKVEKTHV
                     VVNQWCETGVSGIYAIGDLVGPPWLAHKAMHEGVIVAERIAGVAGVHPLDVSKVPGCT
                     YCWPQVASVGLTEARARETGRKIKTGKFPFIGNGKAIALGEVEGFIKTVFDAETGELL
                     GAHMIGAEVTELIQGYTIARNLEATDAELQTTIFPHPTLSEMMHEAVLAADGRPLHI"
     gene            665..>1663
                     /locus_tag="VGM72_08810"
     CDS             665..>1663
                     /locus_tag="VGM72_08810"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY4265405.1"
                     /translation="MVRRTQVKLTRRNVLAAAAGSAALVAGFRGARAQDASKPNIVYI
                     MADDMGYADVSCYGRPDIQTPNIDGIAADGVRFLQGYANSAVCSATRTGLITGRYQYR
                     LRVGLEEPLPVARPDIGLPPEHPTLPSLLKKAGYGTTLIGKWHLGTLPKFGPLKSGYD
                     HFYGFRGGAIDYFAHQGTDHKDDLWNDDAAIRETGYSTALFGQRAVDSIDAYARARQP
                     FLISLHFNAPHWPWEAVDDQVESERLRTKALTDYDGGTQRTYQRMIQAMDSEIGRVMQ
                     ALDANGIAQDTIVIFTSDNGGERFADTWPFTGRKTELLEGGLRIPAIVSWPARIPKG"
BASE COUNT          333 a          481 c          536 g          313 t
ORIGIN      
        1 acgctgaaat cgaaggacgg ccagagcgaa accctcaccg tcgatcgcgt gatcttggcg
       61 gtcggcattg tcggcaatgt cgagaacatc gggctggagc aggtggggat caaagtcgaa
      121 aaaacccatg tcgtcgtcaa ccaatggtgc gagacgggtg tctcgggcat ctacgccatc
      181 ggcgatctcg tcggcccgcc ctggttggcg cacaaggcaa tgcatgaggg cgtgatcgtg
      241 gccgaacgta tcgccggggt tgcgggtgtg cacccgctgg atgtttccaa ggttccgggc
      301 tgcacctatt gctggccgca ggtggcatcc gtcggcctca ccgaagccag ggcgcgcgag
      361 acgggacgca agatcaagac cgggaaattc cccttcatcg gcaatggtaa ggcgattgcg
      421 ctgggcgagg tcgagggctt catcaagacg gtgttcgatg ccgagactgg cgagcttcta
      481 ggcgcccata tgatcggggc cgaagtcacg gagctgatcc aaggctatac gatagcccgg
      541 aacctcgagg cgaccgatgc ggaattgcag accacgatct tcccgcatcc cacactttcg
      601 gagatgatgc acgaagcggt gctcgccgcc gacggcagac cgttgcacat ctgaaagggc
      661 ggacatggtt cggcgcacgc aagtaaaact cacgcgccgt aatgttctcg ccgccgccgc
      721 ggggtctgcc gcgttggtcg cgggttttcg tggcgcacgc gcgcaggatg cgagcaagcc
      781 caatatcgtc tacatcatgg ccgacgacat gggctatgcg gatgtctcgt gctatggccg
      841 ccccgatatc cagacgccga atatcgatgg tatcgcggcg gacggcgtac ggtttcttca
      901 gggttacgcc aattccgccg tatgctcggc cacgcgcacg ggcctgatca cggggcgata
      961 tcagtaccgc ttgcgcgtcg ggctggagga gccgctgccc gtggcacgcc ccgatatcgg
     1021 gcttccgccg gaacatccga cccttccgtc attgttgaag aaggccggtt acgggaccac
     1081 gctgatcggc aagtggcatt tgggaacgct gccaaaattc ggtcccctca aaagcggtta
     1141 cgatcatttc tatggtttcc gcggcggggc catcgattat ttcgcgcatc aggggaccga
     1201 tcataaggac gatctgtgga atgatgacgc ggccattcga gagactggct attcgaccgc
     1261 attgttcggc cagcgcgcgg tggattcgat cgatgcctat gcgcgggccc gtcagccatt
     1321 tctcatcagc ctccatttca atgcgccgca ttggccatgg gaggcggtgg acgatcaggt
     1381 ggaatcggaa cgcctgcgca caaaggccct gaccgattat gacggcggca cgcagcgcac
     1441 ctatcaacgc atgatccagg cgatggattc ggaaatcggc cgcgtcatgc aggcgctcga
     1501 tgcgaacgga atcgcgcaag acacaattgt gatcttcacc agcgacaatg gcggggagcg
     1561 tttcgccgac acttggccgt tcacgggacg caagaccgaa ctgttggaag ggggattgcg
     1621 catacctgcg atcgtgtcct ggccggcgcg gatcccgaag ggt
//
LOCUS       DASYEG010000194         1830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3249262, whole genome shotgun sequence.
ACCESSION   DASYEG010000194 DASYEG010000000
VERSION     DASYEG010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3249262"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1830)
                     /locus_tag="VGM72_08815"
     CDS             complement(<1..>1830)
                     /locus_tag="VGM72_08815"
                     /inference="COORDINATES: protein motif:HMM:NF016768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HEY4265406.1"
                     /translation="RFSTNTFPSWRLAHPYRFIAHNGEINTVRGNVNWMNARRRTMES
                     DLLGPDLDKMWPLIPHGQSDTACVDNALELLVAGGYPLAHAMMMLIPEAWSGNPQMDA
                     KRKAFYEYHAALMEPWDGPADIAFTDGRQIGATLDRNGLRPARFIVTDDDQVIMASEC
                     GVLPIPEEKIVRKWRLQPGKMLLIDTVEGRIIEDEEIKRSLAEAEPYEEWLKNTQFKL
                     HELPELPDAPILAASNEPSALLDRQQAFGYTQEDIQFFLEPMAKDADDPIGSMGADKP
                     IAVLSKRPQLLYNYFHQNFAQVTNPPLDANREELVTSLVSIIGPRPNLFGRDAGSHKR
                     LEVAQPVLTNADLEKIRSISELLDGAFRTATLDCSWPASEGAAGLETAIDRVCREATD
                     AVLADNNILILCDRTVSADRIPIPALLSTAAVHHHLIRQGLRMQTGLVVETGEAREVH
                     HFCALAGYGAEAINPYLAFETLEQTRIQNGLPLKPYEMQKNYIKAVGKGLLKVMSKMG
                     ISTYQSYCGAQIFDAVGLSSSFVEKYFTGTATPIEGVGLKEIAEECVRRHRDAYGDAL
                     IYRNMLDVGGDYAYRIRGEEHAWTPESVAKLQHAVRGKLPDEY"
BASE COUNT          340 a          563 c          559 g          368 t
ORIGIN      
        1 tcgtactcgt ccggcaattt gccgcgcacg gcatgctgta gcttggcgac cgattccgga
       61 gtccaggcat gctcctcgcc gcggatacga taggcgtaat cgccgccgac atcgagcatg
      121 ttgcgataga tcagcgcatc gccataggcg tcgcgatggc ggcgcacaca ctcctcggcg
      181 atctccttga gacccacgcc ttcgatgggc gtcgccgtgc cggtgaaata tttctcgacg
      241 aaactcgacg aaagccccac cgcgtcgaaa atctgcgcgc cgcaatagga ctgataggtc
      301 gagatgccca ttttggacat caccttgagc agccccttgc ccaccgcctt gatgtaattt
      361 ttctgcatct catagggctt caacggcagc ccgttctgaa tgcgcgtttg ctccagcgtc
      421 tcgaaggcga gataggggtt gatcgcttcg gcgccatagc ccgcgagcgc gcagaaatga
      481 tgcacctccc gcgcctcgcc ggtctccacc acaagtccgg tctgcatgcg caggccctgg
      541 cggatcaggt gatgatgcac ggccgcggtc gacagcagcg ccggaatggg aatgcgatcc
      601 gccgataccg tccggtcgca caggatcagg atgttgttgt cggccaatac cgcgtccgtg
      661 gcttcgcggc agacgcggtc gatcgccgtc tccaaaccgg cggcaccttc ggacgccggc
      721 catgagcaat cgagcgtcgc ggtgcggaag gcgccgtcga gcaattccga aatgctgcga
      781 attttttcca gatccgcatt ggtcagcacc ggctgggcga cttccaggcg cttatgcgag
      841 cccgcgtcgc gcccgaatag attgggtctc ggcccgataa tcgagacaag cgaggtcacc
      901 agctcttcgc gattggcgtc gagcggcgga ttggtcacct gcgcaaaatt ctgatggaaa
      961 taattgtaga gcagttgcgg ccgcttcgag agcacggcaa tgggtttgtc cgcgcccatc
     1021 gaaccgatag gatcgtccgc atccttcgcc atcggttcga ggaagaactg gatgtcctcc
     1081 tgcgtgtaac cgaacgcctg ctggcggtcg agcagtgccg agggctcgtt cgatgccgcg
     1141 aggatcggcg catccggcag ctcgggcagt tcatggagct tgaactgcgt gttcttcagc
     1201 cattcctcgt aaggttcggc ttcggcgagg ctgcgcttga tctcctcgtc ttcgatgatg
     1261 cggccttcga ccgtgtcgat caaaagcatc ttgcccggct gcagccgcca tttgcgaacg
     1321 atcttttctt cgggaatcgg cagcacgccg cattccgaag ccatgatgac ctgatcgtcg
     1381 tcggtgacga tgaagcgcgc cggacgcaac ccattgcggt cgagcgtcgc gccgatctga
     1441 cgcccatcgg taaaggcgat gtcggccggc ccatcccagg gctccatcag tgcggcgtga
     1501 tattcgtaga acgccttgcg cttcgcatcc atctgcggat tgcccgacca ggcttccggg
     1561 atgagcatca tcatcgcgtg cgccaacgga taaccgccgg ccaccagaag ctcgagcgca
     1621 ttgtccacac atgccgtgtc cgattggccg tgcgggatca gcggccacat cttgtcgagg
     1681 tccggaccga gaagatccga ttccatcgtg cggcggcggg cattcatcca attgacgttg
     1741 ccgcgcaccg tgttgatctc gccattatgg gcgatgaagc gataggggtg ggcaagccgc
     1801 caggacggga atgtgttggt cgagaagcgc
//
LOCUS       DASYEG010000195         4832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3685503, whole genome shotgun sequence.
ACCESSION   DASYEG010000195 DASYEG010000000
VERSION     DASYEG010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3685503"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VGM72_08820"
     CDS             complement(<1..888)
                     /locus_tag="VGM72_08820"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HEY4265407.1"
                     /translation="MNVAKSPRLLVKARTLSAPRGLAAGRLAFTAKPLMPDIDRTHTG
                     GLGAAAPSQWHEVTLSQWQGSVWDAAHAFSGSAGLGAAAGVEFVEPDLQQQWLYDGDR
                     SRETALAAASCAAADAQNKQFPTLPNDDWFRDDGHGEFLAALKALGLNPPVVRVAHLD
                     TGYDPAHLALPEAPKQLRTDLARNFVDDPPTSDATDRSSGVLNNRGHGTGTMSLLAGR
                     DPKDRTKTIGAAPFVEVVPIRVADRVVLFSNGAIAKALNYVYALCDNEQTRIHVVTMS
                     MGGVASQAWADAINALYDRG"
     gene            complement(998..1294)
                     /locus_tag="VGM72_08825"
     CDS             complement(998..1294)
                     /locus_tag="VGM72_08825"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEY4265408.1"
                     /translation="MAKAYWITAYRSISSPDALAAYAKIAAPALIAAGGRFLARGTAV
                     KTMEQGLMQRTVLIEFDSVDKAVAAYESPGYKEALAALGKSAAERDIRIVEGLE"
     gene            1513..2100
                     /locus_tag="VGM72_08830"
     CDS             1513..2100
                     /locus_tag="VGM72_08830"
                     /inference="COORDINATES: protein motif:HMM:NF014696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEY4265409.1"
                     /translation="MLFLLSPWAGVLFAPLCLWCLLFFRDPERVTPEGEGLITSPADG
                     RLLPVALAVPPVELGMGPEPRMRLSIFMDLFNVHVNRVPSDGTVIALSYRKGRFINAS
                     FDKASEHNERMSARIRLKGLGDARDLAVVQIAGLIARRIKCDLVQDQSVRRGERFGII
                     RFGSRLDVYLPPGANVLVMPNQHVKAGETVLATFQ"
     gene            2117..2950
                     /gene="pssA"
                     /locus_tag="VGM72_08835"
     CDS             2117..2950
                     /gene="pssA"
                     /locus_tag="VGM72_08835"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEY4265410.1"
                     /translation="MARERINPRQWASDHLEDMSISKVIPSALTLLGLCSGATAIKFA
                     LIQEWRSAVAAIFCAMVFDMLDGQAARWLGADSRFGGQLDSLADLVSFGLAPAVVVYS
                     WSLSQMGTAGWIGALIFCICSAIRLARFNIEAARDEGATQSHPYFTGLPTPGAAGVLL
                     LPLLLTFEFPGDLFRDPMFSLTLIALTSILMVSRVPTPCLKHTRLDRNGRIAAVAFVG
                     VLVPLAVYVPWAVLITGLLIYLATIPFVISTLGLKPTATPPLFAEPEESDDLENVIPP
                     G"
     gene            complement(2961..3530)
                     /locus_tag="VGM72_08840"
     CDS             complement(2961..3530)
                     /locus_tag="VGM72_08840"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY4265411.1"
                     /translation="MKTNPLKEELQFLRGLVANPLSVGAVAPSSVWLARAVAAQMDLN
                     QAGPILELGPGTGAITEAILRRGVPPDRLTVIEYDPAFARQIAARFHGVNVILGDAFE
                     LGKLLAERGSQRFSAIVSGIPLLNFTPEKRRSLLEGSFEHLRPGGPFVQFSYGFQAPV
                     SAPAGATARLAAFVWRNIPPARVWVYSRA"
     gene            complement(3705..>4832)
                     /locus_tag="VGM72_08845"
     CDS             complement(3705..>4832)
                     /locus_tag="VGM72_08845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY4265412.1"
                     /translation="CDAFYASVETRDDPSLKGKPLIVGGGKRGVVLTASYEARKFGVK
                     SAMPMFQALKRCPHATIVPPTMSKYAAVARDIRGLLLGLTPLVEPLSLDEAYLDLSGT
                     ARLHGMTPAKTMAMLAKRIEENLGITVSIGLSYNKFLAKLASDLDKPRGFTVIGRTEA
                     RGFLHGRPIGLIRGVGPVLERRLLGDGLTRIGQLQDMSVQELTARYGETGSWLHRLAN
                     GEDARAVDPEGEAKSVSAETTFERDIAGLAELESILWDQAERVSARAKAAGIGGRTVV
                     LKLKTASFRIRTRSESLQAATQLCDVIFRVAVAMLRREATGTPFRLLGIGLSQIRPQS
                     ECDPPDLVDRDAGRRAAAERAIDNVRAKFGKDAVVKGRSMR"
BASE COUNT          863 a         1531 c         1515 g          923 t
ORIGIN      
        1 gccccgatcg tagagggcgt tgatcgcatc cgcccatgcc tgcgatgcca cgccacccat
       61 gctcatggtg accacgtgaa tgcgcgtctg ttcgttgtcg cagagcgcgt agacataatt
      121 gagagccttg gcgatggccc cgttcgagaa caagaccacc cggtcggcga cacggatcgg
      181 caccacctcc acaaacgggg ccgcgccgat ggtcttagta cggtccttgg gatcgcgccc
      241 ggcgagcagg ctcatcgtgc ccgtgccatg accgcgattg ttcagcacgc cgctgctccg
      301 atccgtcgca tcgctggtgg gcggatcgtc gacaaagttc cttgcgagat ccgtcctgag
      361 ctgtttcggc gcctcgggta gcgcaagatg agcgggatcg tatccggtat ccaggtgcgc
      421 gacccggacc acgggcggat taaggcccaa cgctttcagc gcggcgagga attccccatg
      481 accgtcgtcg cgaaaccaat cgtcgtttgg aagcgtgggg aattgcttgt tctgggcgtc
      541 ggccgccgca cagctcgccg ccgcgagcgc ggtttcgcgg ctgcggtccc cgtcatatag
      601 ccattgctgc tgcagatcgg gttcgacaaa ttcgacgccg gcggcggcac ccaatcccgc
      661 cgaaccggaa aacgcgtggg ctgcatccca aaccgaccct tgccattggc tgagcgtgac
      721 ttcatgccat tgcgagggtg cagccgctcc caacccgccc gtatgcgtcc gatcgatgtc
      781 cggcatcaga ggcttcgccg tgaacgccag ccggccggct gcaagtcccc tcggagcgga
      841 cagcgtgcgc gccttcacga gcaagcgcgg cgatttggcc acgttcatgt tccaggtcca
      901 ggtttcgtcg cgagcggata agaaaacaaa aacgactcgt tcgggatttc aacataatcc
      961 cggccgggcg gaacgcctac gccgcggcct ccgcgaatca ctccagccct tcgacgatgc
     1021 ggatatcgcg ctccgcggcg ctctttccca gggcggcgag cgcttccttg tagccggggc
     1081 tctcataggc ggcgaccgct ttgtccacgc tgtcgaactc gatcagcacg gtgcgctgca
     1141 tcaagccctg ctccatcgtc ttgaccgccg taccgcgcgc caggaatcgc ccgccggccg
     1201 cgatcaacgc gggcgcggcg atcttggcat aggcggccag cgcatcggga ctggaaatgg
     1261 aacggtaggc ggtgatccaa taggctttgg ccatgttttt ctcctgctcg ggattgttcc
     1321 gccggccagt gtagtcaccg gcacctcatc tgggcagggg gttgcttgcc cctattggcg
     1381 aagcgacgta ccaagctcga ccgggcgcgg ccggggcaaa atcaagccaa tcatatcgga
     1441 caggagcgaa tgaggattcc aattcatcgg gagggctggc cgtatatcgg ggcttttttc
     1501 gcaacagacg ccttgctgtt cctgctttcg ccctgggccg gcgtgctgtt cgcgcccctc
     1561 tgcctgtggt gcctcctctt cttccgcgat ccggaacgcg tgacgcccga gggcgaggga
     1621 ctgatcacaa gtccggcaga tggacgtttg ctgcctgtcg ctcttgccgt gccgcctgtc
     1681 gaattgggga tggggcccga gccgcgcatg cgcctgagca tcttcatgga tctcttcaac
     1741 gtccatgtga accgggtccc gtcggatggg acagtgattg ccctcagcta ccgtaaggga
     1801 cggtttatta atgcttcttt cgataaagca agcgaacata acgaacggat gtccgcccga
     1861 atccggctaa aaggattagg ggacgcgagg gaccttgccg tggtacaaat cgcgggcttg
     1921 atcgcgcgcc ggatcaaatg cgacctcgtg caggatcaaa gcgtgcgtcg cggcgagcgg
     1981 ttcggcatta tccggtttgg cagccggctc gacgtgtatc tgccgccggg ggcaaatgtg
     2041 ctggtcatgc ccaaccagca tgtcaaagct ggcgagaccg ttctcgcgac ctttcaatga
     2101 tgggattccg acctcgatgg cgcgtgaaag aatcaacccg agacaatggg cttccgacca
     2161 tctcgaagac atgtccatca gcaaggtcat acccagcgct ctgacgctgc tcggcctctg
     2221 ctcgggtgcg acggcgatca aattcgccct gatccaggaa tggagaagcg ccgtcgccgc
     2281 gattttctgc gccatggtct tcgacatgct ggacggccag gccgcacgat ggcttggggc
     2341 cgatagccgc ttcgggggac aactcgattc gctggcggat ctggtcagct tcggccttgc
     2401 tccggccgtt gtggtctatt cctggagcct gtcgcagatg gggaccgcgg ggtggatcgg
     2461 cgcattgatc ttttgcatct gcagcgcgat ccggctggcg cgcttcaata tcgaagccgc
     2521 gcgggacgaa ggcgcgaccc aatctcatcc ctacttcacc ggtcttccga cacccggcgc
     2581 cgcgggtgtt ctcctgctgc cgctgctttt gacgttcgag ttcccgggcg acctgtttcg
     2641 cgacccgatg ttctctctga cgctgatcgc gctcacctcg atcctgatgg tcagccgggt
     2701 accgacgccg tgtttgaaac acacaagact tgatcggaac ggcaggatcg cagcggtggc
     2761 attcgtgggc gtgcttgtcc ctctggcggt ttatgtgccg tgggccgtgc tcatcaccgg
     2821 acttttgatc tatctcgcca caatcccgtt cgtcatctcc acattggggc tgaaacccac
     2881 ggcgacgcct ccacttttcg cggagccgga ggagagcgac gacctggaaa acgtgatccc
     2941 gccaggctga gccaaacggc tcaggcgcgg ctgtaaaccc agacgcgggc gggcggaatg
     3001 ttccgccaca caaaggccgc cagccgggcg gtcgcccccg caggtgccga aaccggcgcc
     3061 tgaaacccgt aggaaaactg gacaaaaggt ccaccgggcc gcaaatgctc gaacgagccc
     3121 tcgagaagcg agcgccgctt ctccggcgtg aagttcagca gcggaatgcc ggaaacaatc
     3181 gcggagaacc gctgagaacc acgctcggcc agcaattttc ccagttcgaa cgcgtcgccc
     3241 agaatgacat tcaccccgtg aaatctggca gcgatctgcc gggcaaaggc gggatcgtat
     3301 tcgatgaccg tcagccggtc cgggggaacg ccgcgccgca aaatggcttc cgtgatagcg
     3361 cctgtcccgg gccccagttc gagaataggc ccagcctgat tgaggtccat ctgggcggca
     3421 acggctcggg cgagccagac gctcgatggg gcaacggccc cgacactcag tgggttagca
     3481 accaatcccc gcaggaattg cagctcttct ttcaagggat tggttttcat gcgggattga
     3541 aatgccgagc tgaggtgaag gcggggaagc cgctagaggt taccgtgtac cggttcgatt
     3601 gcccaaccgg caacagggat ttttgggccg cgaaaccggc gcgggtgaac ttcgcgcttg
     3661 cccgaaggtt tccggccgag agccatgccc gcgaagacgg cggctcagcg catgcttcgg
     3721 cccttgacaa cggcatcctt gccgaatttc gcgcgcacat tgtcgatggc gcgttcggcc
     3781 gcggcacgcc ggcccgcatc gcgatcgaca agatcgggtg ggtcgcattc cgattgcgga
     3841 cggatttggc tcaatcctat gccgagcagc cggaacggcg tgccggttgc ttcgcgccgc
     3901 agcatcgcga cggccacacg gaagatgaca tcgcagagtt gcgtcgcggc ctggaggctc
     3961 tcgcttcgcg tccggatgcg gaatgacgcc gttttcagct tcaaaacgac cgtgcgcccg
     4021 ccgatccccg ccgccttggc tcgcgccgag acacgctcgg cctgatccca caggatgctt
     4081 tccagttcgg caagaccggc gatgtcgcgt tcgaacgtgg tttccgccga cacactcttg
     4141 gcttcgcctt cgggatcgac ggcgcgagcg tcctcgccat tggcgagacg atgaagccag
     4201 gagccggttt caccgtagcg tgccgtcagc tcctgcacac tcatgtcctg gagctggccg
     4261 attcgcgtca gcccatcgcc cagcaggcgc ctttccagca ccggccccac gccacgaatc
     4321 aggcctatgg gccgaccgtg aaggaagcct cgggcttccg tgcggccgat aaccgtgaag
     4381 ccgcgcggct tgtcgaggtc ggaggcgagc ttggccagga atttgttgta ggacaggcct
     4441 atcgaaacgg tgatgccgag attttcctct atccgcttcg ccagcatcgc catggtcttg
     4501 gccggcgtca tgccgtgcag gcgcgccgtg ccggagagat cgagataggc ctcgtccagc
     4561 gacagcggtt cgaccagcgg cgtaagacca agcagcaggc cgcggatgtc gcgcgcgacg
     4621 gcggcatatt tgctcatggt cggcggcacg atggtggcgt gcggacatcg tttcaacgcc
     4681 tggaacatcg gcatcgcgga cttgacgccg aatttgcgcg cctcgtagct ggccgtgagc
     4741 acaaccccgc gtttgccgcc tcccacgatc agcggtttgc ctttcagcga gggatcgtcg
     4801 cgcgtttcca cgctggcgta gaaggcgtcg ca
//
LOCUS       DASYEG010000196         3636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_932725, whole genome shotgun sequence.
ACCESSION   DASYEG010000196 DASYEG010000000
VERSION     DASYEG010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932725"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VGM72_08850"
     CDS             complement(<1..648)
                     /locus_tag="VGM72_08850"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY4265413.1"
                     /translation="MEELLGMLRAAGDATRLRLLLLLTKAELTVSELIQILGQSQPRV
                     SRHLKLLCEAGLLERFKEGAWVFYRAADSYPQAELARALAALADAGADPVLLDDRRRL
                     AQVRAVRAEEAAAYFRANAPQWERIRSLHVPEADVEAAIVRLLGRGSVDFVLDAGTGT
                     GRMLELLAPYIRRGVGIDASPDMLAIARDRLGQAGLSNCQVRRGDVYRLPFPDGNA"
     gene            852..1562
                     /locus_tag="VGM72_08855"
     CDS             852..1562
                     /locus_tag="VGM72_08855"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEY4265414.1"
                     /translation="MITFVGCVLLSGCAAVRNTDGPQDINDPFESFNRASFNVTLAVD
                     KAVYHPVAAGYRRILPTPVRDVVRNFLNNLDSPVIFTNDILQGAMDRAGVTLARAGIN
                     TTLGLGGLVDVASRWGYMRHSEDFGQTLAVYGVGEGPYVFVPLLGPGNPRDILGWGVD
                     LAFDPLTYAQWREKQWWQIGRAGVDFVDLRARNLETLDDVERTSLDFYASVRSLYRQT
                     RNNEIRNGATEVQDLPDF"
     gene            1678..2373
                     /locus_tag="VGM72_08860"
     CDS             1678..2373
                     /locus_tag="VGM72_08860"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4265415.1"
                     /translation="MVSRVFQMPLLTRLASALALLAAICVVAPGAHAAAANAAGAAET
                     FVQQNIDKGYVILNNTSLPQDQRRMQFRNFMLGLTDVKRIGMFTLGQYANGASKADID
                     AFTNAFTDYAIAVYESRLSKYKGQTLKVTGADVRSPDDVVVNADVVNPSAPPNAPVIK
                     AAFRVRKTADDKPIITDMQVEGIWLALSQRSDFTGFLQQHGGSLAALTDNLKTQVQQL
                     RASNENGSITRKT"
     gene            complement(2437..>3636)
                     /gene="ettA"
                     /locus_tag="VGM72_08865"
     CDS             complement(2437..>3636)
                     /gene="ettA"
                     /locus_tag="VGM72_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEY4265416.1"
                     /translation="ADVTKLSGGEKRRVALCKVLLEEPDMLLLDEPTNHLDAASVSWL
                     ERHLSDYAGTVVLVTHDRYFLDDVTGWILELDRGRGIPHEGNYSTWLDMQEKRLAQES
                     EHEEARQRALQRESAWIKTSPRARQAKSKARIQAYEELLLKAQDNRGGTAQIVIPAGP
                     RLGSLVVESEGVGKAYGDQLLFENLEFKLPPGGIVGVIGPNGAGKTTLFRLLTGQETP
                     DTGALRIGDTVVVGYVDQSRDSLKPNATVWEEISGGLNVLELGKRTMPSRAYVGAFNF
                     KGGDQQKKVGQLSGGERNRVHLAKMLKSGANLLLLDEPTNDLDVETLRALENALEDFA
                     GCAMIISHDRWFLDRIATHILAFEGESHVEWFEGNYQDYEADRKKRFGIDADTPHRIK
                     YKRFARA"
BASE COUNT          708 a         1132 c         1065 g          731 t
ORIGIN      
        1 cgcgttgccg tcggggaaag gaagccgata cacgtcgccg cggcgcacct ggcaattgga
       61 taatccggcc tggccgagcc ggtcgcgcgc gatggcgagc atgtcggggc ttgcgtcgat
      121 gcccacgcca cgccggatgt aaggtgccag caattcgagc atccgccccg tcccggtgcc
      181 cgcgtcgagg acaaaatcga cagacccgcg gccgagcaaa cgcacgatcg ccgcttccac
      241 atccgcctca gggacatgaa gcgagcggat gcgctcccat tgcggtgcat tggcccggaa
      301 ataggcggcc gcctcctcgg cgcgcacagc gcgcacctgg gcaaggcgcc tcctgtcgtc
      361 cagaagaacc ggatcggccc cggcatcggc gagggcggcg agagcgcgtg ccaattccgc
      421 ctgcggatat gaatcggccg cgcggtagaa aacccaggca ccctccttga accgttcgag
      481 cagcccagct tcgcagagaa gcttcaaatg ccggctgacc cgcggctggc tctggcccag
      541 aatctggatc agctcactca cggtcaactc ggctttggtc aaaagcagca atagacgcaa
      601 cctcgtcgcg tcaccggctg cccgcaacat ccccaagagc tcttccacgt ggcttacttc
      661 cctatcaggg ccacatcata taaacatatg tttatatgat tatgcaatcc tgatacaaat
      721 attgccggtt ccggtcaagc tcccgcaagc gctctgtgct agacatggtt accagggggg
      781 atggcattcg gacgtgcgtc ggtgggattc acacaggcaa ttttggcaaa atgagtatct
      841 gcaaagggat aatgatcaca ttcgtcgggt gcgtcctgct gtccgggtgc gctgcggtgc
      901 gcaataccga cggtccccag gacatcaacg atccattcga atctttcaac cgggcgagct
      961 tcaatgtcac gctcgcggtg gataaggcgg tctatcatcc ggtggctgcc ggctatcgcc
     1021 gcatcctacc cacaccggtt cgcgacgtgg tgcgaaactt cctcaacaat ctggattcgc
     1081 ctgtcatttt caccaacgac atcctgcaag gcgcaatgga tcgcgctggg gtaacgctcg
     1141 caagggccgg gatcaacacg acgctcggcc ttggcggcct tgtcgatgtc gcgagccgtt
     1201 ggggctatat gcgccattcc gaggatttcg gtcagaccct ggcggtctat ggcgtgggcg
     1261 aaggccccta tgtgttcgtg ccgctgttgg ggcccggcaa tccgcgtgac atcctggggt
     1321 ggggggtcga tctcgcgttc gatcctctca cctatgcgca gtggcgcgaa aagcaatggt
     1381 ggcaaatcgg ccgcgccggc gtcgatttcg tggatttgcg cgcccgcaat ttggaaactc
     1441 tcgatgacgt cgagcggacg tcgctggatt tttacgcctc cgtgcgcagc ctttatcgtc
     1501 agacccgaaa caacgagatc cgcaacggcg ccacggaagt ccaggatttg cctgattttt
     1561 agccggccgc gcgcaggttg tgcacaggaa cgaagcctgc ccctacacgt ttgccattta
     1621 agcgccatat atctttcgaa cgtttccagc ctcaaggctg tttctgggag aacatcaatg
     1681 gtttcgcgtg tatttcaaat gccgcttttg acgcgcttag cctccgcgct ggccttgctt
     1741 gccgccattt gcgtcgttgc gccaggggct cacgcggccg cagccaatgc agccggcgcg
     1801 gctgaaacct tcgtgcagca gaacatcgac aaaggttatg tgatcctcaa caacacctcg
     1861 cttccccaag atcagcgccg gatgcaattc cgtaatttca tgctgggtct gacggatgtg
     1921 aaacgcatcg gcatgttcac gctcggccaa tatgccaatg gagcttcgaa ggcggatatc
     1981 gatgccttta ccaacgcgtt caccgattac gccattgcgg tctatgaatc gcggctgagc
     2041 aagtacaagg gtcagacgct gaaggtcacg ggcgcggacg tgcgctcgcc ggatgacgtc
     2101 gtggtcaacg ccgatgtggt caacccgtcc gcgcccccca acgcgccggt gatcaaggcc
     2161 gcattccgcg tgcgcaagac cgccgacgac aaacccatca tcacggatat gcaggttgaa
     2221 ggcatttggc tggcgctctc gcagcgctcc gatttcaccg gcttcttgca gcagcatggc
     2281 ggcagtttgg cggcgctgac cgataatctg aaaacacagg tacagcagct ccgtgccagc
     2341 aatgaaaacg gctccataac gcgaaaaacg taacctcgtc gctgccgccg ataaacaaaa
     2401 ggccgcataa atgccggcct ttttctttgc tcgtttctat gccctggcga aacgcttgta
     2461 tttgatgcgg tgcggcgtat cggcgtcgat gccgaagcgc ttcttccggt ccgcttcata
     2521 gtcctggtaa ttgccctcaa accattcgac gtgggactcg ccctcgaagg ccaggatatg
     2581 cgtcgcgata cggtccagga accagcgatc atggctgatg atcatggcgc agccggcgaa
     2641 atcctccagc gcgttttcga gcgcgcgcag ggtttcaacg tcgagatcgt tggtgggttc
     2701 gtccagcagc agcagattgg cgccggactt cagcatcttg gccagatgca cgcgattgcg
     2761 ctcgccgccc gaaagctgcc ccaccttttt ctgctgatcg ccgcccttga aattgaaggc
     2821 ccccacatag gcgcgtgacg gcatggtgcg cttgcccagc tccagcacgt tgagaccgcc
     2881 ggagatttcc tcccagacgg tggcgttcgg cttcaacgaa tcgcgcgact gatcgacata
     2941 gcccacgacg accgtgtcgc cgatccgcaa cgcgccggtg tcgggtgtct cctgcccggt
     3001 caaaagacgg aacaatgtcg tcttgcccgc gccattgggg ccgatgacgc cgacaatccc
     3061 gcccggcggc agtttgaatt ccagattctc gaacaacaac tgatcgccat aggctttgcc
     3121 gacaccttcg gactcgacca ccaacgaccc gagccgaggc ccggccggga tcacgatctg
     3181 tgccgtgccg ccgcgattgt cttgcgcctt cagcagcaac tcctcatagg cctggatgcg
     3241 cgccttggat ttggcctgac gcgcgcgcgg gctggttttg atccaggcgg attcacgctg
     3301 cagcgcgcgc tggcgggctt cttcgtgttc gctttcctgg gccagacgct tttcctgcat
     3361 gtccagccag gtggaataat tgccttcatg aggaatgccg cgtccgcgat cgagttccag
     3421 aatccagccc gtcacatcgt cgaggaaata gcggtcatgg gtcaccagca cgacggtgcc
     3481 ggcgtaatcg gacagatgcc gctccagcca ggagaccgat gcggcgtcca gatggttggt
     3541 gggctcgtcc aggagcagca tgtcgggctc ctcgagcagg accttgcaga gcgcgacgcg
     3601 ccgcttttcg ccaccggaaa gcttcgtgac gtcggc
//
LOCUS       DASYEG010000197         6509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1075854, whole genome shotgun sequence.
ACCESSION   DASYEG010000197 DASYEG010000000
VERSION     DASYEG010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6509
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075854"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(420..3425)
                     /locus_tag="VGM72_08870"
     CDS             complement(420..3425)
                     /locus_tag="VGM72_08870"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HEY4265417.1"
                     /translation="MPVRIIAKRSPLACLLLATIATPALADDLSVTTILTSPVATSKA
                     SNGTPGNISITDTGGISTNAAGAALTLDSNNTVESLGVIQNAFAGGAIGVHIVGGNTG
                     SFITDVTTTSLINVSGGGDGNYGVLVDGSAPFTGDIDLGVVSTIAVNGSNSSGVAIKT
                     PLIGNLSVGATTAATGAGADGVLVVAPITGTLTLGFSVSAVGTNLFTTDKIDPLAGAA
                     VAIGSSISNGILNAGPSTVDDGTTSSSLVSAGSLPTLAIQPSIAGAGATNITIGALSS
                     DTVNPTFSLVNRGSVRATVNDPGISTIGIGIGELGTAAHTVTLAGGIYNRGSIQAQAE
                     SDNIFATSAAPASADATGLLIGNGATINAANLSTVALLSEGSISVSETGNQPGTATAV
                     LIQPGGTLPSFTNTGAINSLITTTDTTIASLSAYGVRDLSGTLRTVVNSGIWLAQAST
                     LDNGAQKAIAADLSHTAQNETFTNSGSIVGDIVFGSGANQLVIEGGTTIGTCSAACVQ
                     GSVHATGTGTLDVHVSEGGTGGIFRTPSAQLSSLNVGSAGTVEFAIDKNSTATPFITT
                     TGPVTFNPGSKALLVPTTFLPSSGTYTLIHSGGGLTFADFATATAQPIPFLFNGSITQ
                     QNNDLLLTLTRKSATEIGLTGNAAAIYEPLAAAALSGDDAFGAALLSFGTAAEVQTAL
                     NTVVPDVAGGVRALTIAMTDQATGVIGARERALVTAPEGTRNEFRFWGQEFYNNVSGD
                     STAVAPGYSGAGQGVAMGVEWGNIGTGRYGVSYTFFSSQETEAHPLDTKTNGDWNLVS
                     FYAGWRRGAFFVTPEVNVGEGNFQSRRTIVAGSSLIRASTSDWSSYLAAGGFTTGYIF
                     DLGGFQVIPQIALDGLHLHEGTYNEVGGGGVGLSLKSQNQESVRSFAGIVGQGLYAWN
                     TGNLQPQLLVGWSHEFMDSPATIDGSFEAAPGSPFHLVGPTVEPNKIIGGASFAYIMG
                     NWAAGVNYDASASTGTLAQSATISLSSRF"
     gene            3602..4915
                     /locus_tag="VGM72_08875"
     CDS             3602..4915
                     /locus_tag="VGM72_08875"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEY4265418.1"
                     /translation="MDRDKSRRLSCTKLSEKQVAAAAARHELLYSGRMGGARAVFAFC
                     DLSGLDLSGRNLADADFTGAYLEETNLAGARLDSASFFGASLKRANLAGASLRRADMR
                     GTSLRGANLIGADLYEADLREGKIAEKGVSGELKILQHDIGPSELPAALLSSANLERA
                     KLTGVIAMQADFTDAIMRNCRLTRANLRRARMSGANLENADLRGCDLAGADLKGAVLT
                     GAMLEFASLEGCDMSGALTNAPAGRPLSELAEPVEVILRAHARWAETQGREGRPADFS
                     GFDLRSLKGLPHAVLPALIAPKGVFYGLDLSGGRFPGAQLEGADLRGCKLTGADLRGC
                     NLKGAKLGNADLRDVNLGPLLISNERLLPARLERADGRYADFRGADLRQTVFAEADLA
                     YADLTGAKLRAADFRGATLQGIKLSLDAAVDAIFDETRYDTAAAS"
     gene            complement(4919..5803)
                     /locus_tag="VGM72_08880"
     CDS             complement(4919..5803)
                     /locus_tag="VGM72_08880"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4265419.1"
                     /translation="MSHATPVDDLRRRYAALAQAFGPTPADVVAERAHLGQVKGEWVR
                     IAQTDPQRLLLYFHGGGYVSGSPESHRALVGKLCRVAGAAALSLDYRLGPEFPFPAGL
                     RDAVDAYRFLISKAYPPESVVLAGDGAGGGLAVATMMAIRNGGLPMPAGLIAMSPWAD
                     LTLSGWSIMQNAESDPVMSWEMLFVSARHYLKGANPADPYASPVFGSMRDFPPIMIHA
                     GNRELLKDDASRLGELAAASNVPVSVEVYDGMGHLFQAEASQDAKVSLHRLGQFIRTR
                     TPETAPNIDFSKSAKRGT"
     gene            complement(5974..6441)
                     /locus_tag="VGM72_08885"
     CDS             complement(5974..6441)
                     /locus_tag="VGM72_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEY4265420.1"
                     /translation="MRSFDLTPLFRSTVGFDRLADMLDSASQFDTGISYPPYNIERTD
                     ENHYRISLAVAGFAEKDMTVEVKEGVLSVQGKRESEKENHEQRYLYQGIAGRTFERRF
                     QLAENVEVRGAQLENGLLHIELERLVPEEKKPRRIAINTKPEGRMADSKAKAA"
BASE COUNT         1204 a         2011 c         2073 g         1221 t
ORIGIN      
        1 tgcagcgtcg ggacgtaacc gaaaatccca gcgcaatcaa tcgtcctgat gctctgcgac
       61 gaaacgtcca caaaattcga tctatcggca gagggaactt cccgcaagcc tattgctctc
      121 acaacctcgt gctcacgcgc ctggaaccac cataccccgc gcggggagcg gcgccgcggc
      181 tcgcgacaac gcggggtatg agatctggca gagccgcgtc caatcgaagc gctgcgagag
      241 cgatcggccg gcgcgaaacg cgccgccggc cgcgtcataa ttgggcgccc gctcgcgtca
      301 taatcgggtg cgagacgcag cgaatgcgca agggctccag cgtgaacgct ccgtagacgg
      361 aatatacggc gaagccccgg agatgagcgc ggaggaaatg gatgagcgtg cggaaatttc
      421 tagaaacggc tgctcaaact tatggtcgcc gattgcgcca gggttccggt gctggcgctc
      481 gcgtcgtaat tgacgcccgc ggcccaatta cccatgatgt atgcgaaact cgcgccgcca
      541 atgatcttat tcggttcaac cgtgggcccg acaagatgga agggcgatcc cggggccgct
      601 tcgaaactcc cgtcaatggt agccggactg tccatgaatt catggctcca gccgaccaga
      661 agctgaggct gaagatttcc ggtgttccag gcataaaggc cttgtcctac gataccggcg
      721 aagctgcgaa ccgattcctg gttttgcgac ttcagcgaca ggccgacacc gccgcctccc
      781 acttcattgt aggtcccctc atgcaagtgt agaccgtcca aggcaatctg cgggatgact
      841 tgaaagccgc cgaggtcgaa aatgtacccg gtcgtgaagc cgcccgcggc gagatagctc
      901 gaccagtccg acgtggatgc gcggataagg gaggaacccg cgacgatggt acggcggctc
      961 tgaaaattgc cctccccgac attgacttcc ggcgtgacaa agaaagcacc ccgccgccag
     1021 ccggcgtaga acgacaccag gttccagtcg ccattggtct tggtgtcgag tgggtgggct
     1081 tcggtttcct ggctggaaaa gaacgtatag ctgacgccgt aacgcccggt cccgatattg
     1141 ccccattcca cgcccatggc gacgccctgg cccgctccgc tatagccggg tgccactgcg
     1201 gtgctgtcgc cgctgacatt gttgtagaat tcctgacccc agaagcggaa ttcgttccgc
     1261 gtgccctctg gtgccgtcac cagagcgcgc tcgcgcgccc cgatcacgcc tgttgcctgg
     1321 tcggtcatag cgatggtgag ggcccggacc ccgcccgcga cgtccggcac cacggtgttc
     1381 agcgcggttt gcacctccgc agccgtgcca aacgagagca gcgccgctcc aaacgcatcg
     1441 tcaccgctga gcgcggcggc cgccaacggc tcgtagatcg cggccgcgtt tccggtgagc
     1501 ccgatttcgg tcgcggactt gcgcgtcaac gtcagaagca gatcgttgtt ctgctgggtg
     1561 atagagccat tgaagaggaa cggaatcggc tgggcggtag cggttgcgaa atcggcgaag
     1621 gtgaggccgc cgccgctgtg gatcaatgtg taggtgccgc tgctcggcag gaacgtggtg
     1681 ggcaccagca gcgctttcga acccgggttg aacgtcaccg ggccggttgt cgtgataaag
     1741 ggcgttgcgg tcgaattctt gtcgatcgcg aattccaccg tcccggcgct gccgacattg
     1801 agactggaga gttgagcgct cggcgtgcgg aagatgccac cggttccgcc ttcggagaca
     1861 tgcacatcga gcgtgcccgt gccggtcgcg tgaaccgaac cctgcacgca ggccgccgaa
     1921 cacgtgccga tcgtagtccc gccttcgatg acgagctgat tggcgcctga gccgaatacg
     1981 atgtcgccga cgatcgaccc gctgttggtg aacgtctcgt tttgggcggt gtgcgagaga
     2041 tcggccgcga tggccttttg tgcgccattg tcgagcgtgc tcgcctgcgc gagccagatt
     2101 ccggaattga ccaccgtccg cagcgtgccg gaaaggtcgc gcacgccata ggccgacagg
     2161 ctcgcgatcg tggtgtcggt tgtggtgatt aaactgttta tggcgcccgt attggtgaaa
     2221 ctgggcagcg ttccgccggg ctggatgagc accgccgtgg cggtaccggg ctggttgccg
     2281 gtctcggaca cactgatcga accctcacta aggagggcga ccgtcgaaag atttgcggcg
     2341 ttgatcgtgg cgccattgcc gatgagcagt cctgttgcat cggccgatgc cggtgcagcc
     2401 gatgtggcga atatgttgtc gctctcggcc tgggcctgaa tgctgccgcg attatagatg
     2461 ccgcccgcca gcgtgacggt atgggcagcc gtccccaatt ctccgatccc gataccgatg
     2521 gtgctgatcc cgggatcgtt cacggtcgcc cgcacgctgc cccggttgac caggctgaag
     2581 gtcggattga ccgtatcgga ggacaaagca ccgatcgtga tattggtagc gcccgcgccc
     2641 gcgatggagg gctggatcgc caaggtcggg agcgatcccg ccgacaccag cgaagaggag
     2701 gttgtcccgt cgtccacggt cgaaggcccg gcattgagaa tgccgttgct gatgcttgaa
     2761 ccgatcgcga cggcggcccc agccaacgga tcgattttgt ccgtcgtgaa gaggttggtc
     2821 cccaccgcgc tgacgctgaa tccaagcgta agcgtccctg tgataggagc aacgacaagc
     2881 acgccgtcgg cgcccgcacc cgtcgctgcc gtggtggcgc cgacgctcag atttccgatc
     2941 agcggcgttt tgatcgccac gccgctcgaa ttgctcccgt tgaccgcgat ggtggacacc
     3001 acgccgagat cgatatcgcc cgtgaacggc gcgctgccat ccaccagcac gccgtaattg
     3061 ccgtcaccgc caccgctgac attgatcagg ctggtggtcg ttacatcggt gatgaaggag
     3121 ccggtattgc cgcccacgat atggacgccg atggcgccgc cggcgaaggc gttctggata
     3181 acgcccagcg actcgaccgt gttgttggaa tcgagcgtca gcgcggcgcc cgccgcattg
     3241 gtgcttatcc cgcccgtgtc ggtgatcgag atgttgccgg gcgtgccgtt gctggccttc
     3301 gacgtggcga cgggggacgt caggatcgtg gtaacgctca aatcgtccgc gagcgccggc
     3361 gtcgcgatgg tggcaagcaa caggcaggcc aatgggctgc gtttcgcaat aatccttaca
     3421 ggcatttaat tttgctcgat ttttccgaac cggacgcccc tgatgcgggc gaaggtgcca
     3481 tgatggccgc tatgcggcaa gcccataaaa gatcacgggt ttggatagtt cttaaggccc
     3541 tattagcggc attcacggca aaatccgccg gtcgagaagc tttcagcggt gatgccgttc
     3601 catggatcgt gacaagtcgc gtcggctgag ctgtacgaag ctgagcgaga agcaggtcgc
     3661 ggctgccgcg gcgcgccatg aattgctcta ttcgggccgg atgggcggtg cacgtgccgt
     3721 gttcgcgttt tgcgatcttt cgggtctgga cctttcggga cgcaatctcg ccgatgccga
     3781 ttttaccggc gcatacctcg aagagaccaa tctcgccggc gcgcgtctcg attccgcaag
     3841 cttcttcgga gcatcgctga aacgcgccaa tcttgcgggt gccagcttgc gtcgcgccga
     3901 catgcgcggc acctctcttc gcggcgccaa tctgatcggc gccgatcttt acgaggccga
     3961 tctgcgcgag ggcaagatcg ccgagaaggg cgtttcgggc gagcttaaga tcctgcaaca
     4021 cgatatcggt ccatccgagc tgccggccgc gttgctttct tcggccaatc tcgaacgtgc
     4081 caaactgacc ggcgtcatcg ccatgcaggc cgatttcacc gatgccatca tgcgaaattg
     4141 ccgtctgaca cgggccaatc tgcgccgcgc acggatgtcc ggagccaatc tcgaaaatgc
     4201 ggatttgcgc ggctgcgacc ttgccggcgc cgatctgaag ggggcggttc tcaccggggc
     4261 catgctggaa tttgcgtccc tggaaggctg cgacatgtcg ggcgccctca cgaacgctcc
     4321 ggccggaaga ccgctttccg aactggccga acccgtcgag gtgatcttgc gagcccatgc
     4381 gcgctgggcc gagacccagg ggcgcgaagg ccggccggcc gatttctcag gtttcgattt
     4441 gcgctccctc aaaggtttgc cgcatgcagt cctgcccgcg ttgatcgctc cgaaaggcgt
     4501 gttttacggc ctggatctgt cgggggggcg ctttccaggc gcacaactcg aaggcgcgga
     4561 tttgcgcggc tgcaagctca cgggcgcgga tttgcgcggt tgcaatctga agggcgccaa
     4621 gctcggcaat gccgatctgc gcgatgtgaa cctgggtccg ctgctgatct cgaacgagag
     4681 gctgctgccg gcccggctcg agcgggcgga cggccgctat gccgattttc gtggcgcgga
     4741 tttgcgccag accgtgtttg cagaggccga tctggcctat gccgatctca ccggcgcgaa
     4801 attgcgcgcg gccgattttc gcggcgccac cctgcaaggg atcaagctgt cgctcgatgc
     4861 cgcggtcgat gccattttcg acgaaacccg ctacgacacg gcggcggcga gctgaacgct
     4921 acgtgccgcg cttcgcactt ttgctgaaat cgatattggg cgcggtttca ggcgtgcgcg
     4981 tgcggatgaa ctggcccagg cggtggagcg agactttcgc gtcctgcgag gcttcagcct
     5041 gaaacagatg gcccatgccg tcatagactt ccaccgaaac gggcacgttg gacgcggcgg
     5101 ccagctcgcc caggcggctg gcgtcgtcct tgaggagttc gcgattgccg gcatgaatca
     5161 tgatcggcgg aaaatcgcgc atcgaaccga acacgggcga agcgtaggga tcggcgggat
     5221 tggcgccttt caggtaatgg cgcgcgctca cgaacagcat ctcccaggac atcaccggat
     5281 cgctctcggc attctgcatg atggaccagc cggagagcgt caggtccgcc catggcgaca
     5341 tggcaatgag gcccgcgggc atcggcaggc cgccattgcg gatcgccatc atggtcgcca
     5401 ccgcaaggcc gcccccggcg ccgtcacctg cgagcaccac cgattccggc ggataggcct
     5461 tggaaatcag gaagcgatag gcatccaccg catcgcgcaa cccggccggg aacggaaatt
     5521 cggggccgag ccgataatcg agtgaaagcg ccgcagcccc cgccacccgg cacaatttcc
     5581 cgaccagcgc acgatggctt tccggcgagc ctgaaacgta acctccccca tggaaataga
     5641 gcagcagacg ctgcggatcg gtctgcgcga tccggaccca ttcccctttc acctgaccaa
     5701 gatgcgcgcg ctcggccacc acatcggccg gggtggggcc gaacgcctgg gccagcgcgg
     5761 catagcgccg ccgcaaatcg tcgaccgggg tcgcatggct cacccccgac cgcgtcaagc
     5821 gcgataaacg tgggggaaag atgccttgcc agctctgcat gaaaccttcg gtgacgcaga
     5881 aagaaggcca aactgtagcg cgctgcggcg gccgaacaaa ggaaaagcgc gcctcccggc
     5941 tggaaagcgc gcttttgggg gaacttccgg gcgttacgcc gcctttgctt tgctgtcggc
     6001 catccggcct tccggcttgg tattgatcgc gatacggcgg ggcttcttct cctcgggcac
     6061 gaggcgctcg agctcgatat gcaggagtcc gttctcaagc tgggctcccc tgacctcgac
     6121 gttctcggcc aactggaaac gccgctcgaa ggtgcggccc gcgatgccct gatacagata
     6181 acgctgttcg tggttttcct tctcgctttc gcgcttgccc tgtacggaca gcacgccttc
     6241 cttgacctct acggtcatgt ctttttccgc gaagccggcg accgcaaggg aaatccggta
     6301 atgattttcg tcggtgcgtt cgatgttgta gggcggataa gaaatcccgg tatcgaactg
     6361 ggatgccgaa tccagcatgt cggcaaggcg gtcgaaaccg acggtcgagc gaaagagcgg
     6421 ggtcaaatca aaactgcgca tgtcacgttc ctcctgggag cgaagtgtta aatccgggca
     6481 cgctcacaac gagccgaacc ctcattcag
//
LOCUS       DASYEG010000198        19358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1659683, whole genome shotgun sequence.
ACCESSION   DASYEG010000198 DASYEG010000000
VERSION     DASYEG010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 19358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19358
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659683"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1433
                     /locus_tag="VGM72_08890"
     CDS             141..1433
                     /locus_tag="VGM72_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY4265421.1"
                     /translation="MPTTSKHPETIALHAGYRADPSTSAVAVPIYQTTSYQFGSAEHA
                     ANLFALKEFGNIYTRIMNPTQAVLEERIAALEGGAAALALASGQSASLFAVQNICHAG
                     DNFVCSTDIYGGTWNLFANTMKTMGIECRFVDPADPENFRRATDARTRCYYGETLPNP
                     KLDVFPIAEVAAIGRSAGVPLIMDNTAAPVLCRPIAHGAAITMHSTTKYIGGHGTSIG
                     GIIVDAGNFDWEKHAERFPMLNTPDPSYHGAVWTQAVKPLGPIAYIIKARVTLLRDVG
                     AAMSPFNAFMFIQGLETLSLRMRQHCANAVAVAKYLSGHSKVTRVIFPGHQSGESRRR
                     ADAYLKGGYGGLVGFELKGGSDSGRAFIDALTMFYHVANLGDVRSLAIHPASTTHSQL
                     TSDEQMQTGVTDGYVRLCVGIEHIDDIIADLDQALAAA"
     gene            1559..2539
                     /locus_tag="VGM72_08895"
     CDS             1559..2539
                     /locus_tag="VGM72_08895"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY4265422.1"
                     /translation="MSTLSPALDLFLYQFLRFFNPRQGYCWIYLAGGLFFALCVMLWR
                     RRNRSRIRARPFLRLLGSRALWLHRSTLLDVKLYFLHGVLVLIAYGMFEATSEIWRAG
                     AASALAALAGAEPRLHAPHAIVGSLTTMLQIIALDLGYWALHYCFHKVPVLWELHKVH
                     HSAEVMTPLSEWRQHPFEFIAFANVLTMTTGSVYGAMSWLFGAAAQPFTLFQINALFV
                     MHLLTFHHLRHSGVWIAATGGLGRVFHSPAHHQIHHSTDPRHFDRNLGYALSIWDWAF
                     GTLCVPERPLKVHFGVAGEPPYRGMADTLIRPLVAAAAKLQFALVPGRRL"
     gene            2691..3800
                     /locus_tag="VGM72_08900"
     CDS             2691..3800
                     /locus_tag="VGM72_08900"
                     /inference="COORDINATES: protein motif:HMM:NF021603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEY4265423.1"
                     /translation="MSAPHSKLVMLADMAAEASGDARRELLRQIADSFRPENTALNRI
                     NRADLDKIMSAIICEFTIAIRTELSRYIAASPLPFNATARNLALDEISVARPVLERST
                     ALTDQDLLEVIAQKSQAHMTAVAKRAHVSEDVCEALVEYGEDRVVASLLDNAGAKIAY
                     GTYEKVAARAETSVLLQGPFIQRSTVPLDLLNDLYFKVEGDLRRQVLEHFETASKQDL
                     SVALVLSRRRWLVKYGGLPKNYETLSSRIDEFHRRGELKPAMLVGYLRSHLHTEFKLG
                     FAKLTGTDYELVNRLVERNDLDGVAILARAGNFDRAFFVTVAMMLSGGGNRMADAESF
                     GAQYEAISVEQASRVVRMWRVGPESEARQPVRLAS"
     gene            complement(3827..5110)
                     /locus_tag="VGM72_08905"
     CDS             complement(3827..5110)
                     /locus_tag="VGM72_08905"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4265424.1"
                     /translation="MPRIFKTTNIVLVLLCAMYFITYIVRQNVNTAGGPIQQEFGLSN
                     TQLGLIFSAYGIPYLLFQIIGGWTADHFGPRRTLFVSGIVWAGATILTSLAFDFYSLF
                     ALRVLMGFGVGATLPTATRAMQHWVEARKRGFAQGITHAFARLGNAATPLLIAALMSV
                     IEWRGSFIAVGAFGFIWVFVWLWYFRDDPKEHPSITQAELALLPHRPKGPKPIVPWGP
                     LIGRMWPVTITYFCYGWSLWLYLNWLPLFFKNTYSLDIKQSAIFATGVFLAGVVGDTL
                     GGVFSDAIYHRTKNVRLARLSICALGFTGALLSLVPILYIHDINIVALCLTSGFFFEE
                     LVIGPMWAIPMDIAPKYSGTAAGLMNTGSALAAILSPAIAGYVIDRTGNWYLPFIMSI
                     GVLLVGALMTFAMHPERQFTDEGVSMAKPRPAPAE"
     gene            complement(5148..6356)
                     /locus_tag="VGM72_08910"
     CDS             complement(5148..6356)
                     /locus_tag="VGM72_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY4265425.1"
                     /translation="MPTNTPRIAGRHFLQIPGPTPVPDRILRAMDRAVIDHRGPDFQQ
                     LGQRVLSGIKTIFKTRGAVFIYPSSGTGAWEASLTNLLSPGDRVLMYETGHFATLWKT
                     MALKLGVKPEFIESDWRTGVDPAKIEERLRADTNREIKAVCVVHNETSTGVTSPIADV
                     RKALDAAGHPALLLVDTISSLASVDYRHDEWGADVTVAGSQKGLMLPPGLGFTAVSEK
                     AMKAAKAGKLPKSFFSWEDMAGPNASGFFPYTPATNLLYALAEAVDMLHEEGLDRVFA
                     RHKRHAEATRRAVRAWGLEILCSDPKCYSPVLTAILMPAGHDSDAFRKIVLENFDMSL
                     GAGLSKLAGKVFRIGHLGDTNDLTILGALSGVEMGLELAGVPHKKGGAQAAMDYLAKT
                     AKTPMESAAE"
     gene            6444..9392
                     /locus_tag="VGM72_08915"
     CDS             6444..9392
                     /locus_tag="VGM72_08915"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY4265426.1"
                     /translation="MTIVASPGLERRLKSENTGEILFDRFTRGRYATDASSYQMMPLG
                     VVVPRSVDEAVRAIAIARAEGVSVVPRGGGTSQCGQTINHSLVVDCSKYLNKIVSLDG
                     NARTCVVQPGIVLDELNRQLKPHGLWFAVDPSTASRATLGGMTGNNSCGTRSLRYGTT
                     RDNIIAVNAVLPNGAPARFAEVSRDLSELPADSALRPIARALLALGEREAAEIEARFP
                     KVQRRVGGYNLDALVPKNTKTNLAHILVGSEGTLAFSTGIELKLSPLLGRRVMGVCHF
                     GQFYEAMNAAQHLVTLGPIAVEAIDKTMLDLARAIPLFRDVIPKFTREDPEALLMVEF
                     DEGDEENERRMKRLVEMMGDLGFSWDKIGAKWGGVVEVRDAGLQAAIAEVRASGLNIM
                     MSMKDERKPVSFIEDCAVPLVHLAEYTDRLTQVFEKYGTKGTWYAHASVGCLHVRPVL
                     NLKLEKDLKAMRAIAEEAFELVKKFKGSHSGEHGDGIVRSEFHEMMFGTELVRAFGEV
                     KALFDPEKFFNPGKIVGAPKFDDPDYLRFGPAYEISAFKPALDWSEFPGGASGFQGAA
                     EMCNNNGACRKTEGGAMCPSYRATRNERDVTRGRANSLRLALSGQLGADALASDEMME
                     TLKLCVSCKACRRECPTGVDMAKFKIEVLAARAAKHGVSLRDKLIGYLPRYAPWGARF
                     AWLLNLRDQIPGAAALSEPIIGFSAKRSLPKWRTHYFNPAATVIESTLPLVGEVVLFA
                     DTFNRYHESENLDAALAVLTGAGYRVHLPKAVNGNARPLCCGRTFLTAGLVDEARAEN
                     ARSAAALLPFAKRGIPIVGLEPSCIFTFRDEAPAIAPGEDSKLLAKSTFLFEEFLARE
                     IDAGRFNPKLAPIGANALLHGHCHQKAFNVMGPVQKVLSKVPGLKVELIESSCCGMAG
                     AFGYQSETIDVSLKMAELSLLPAVRKASADAIVVADGTSCRHQIHDGTGRTALHVAAV
                     LAKSMAAANA"
     gene            complement(9415..9915)
                     /locus_tag="VGM72_08920"
     CDS             complement(9415..9915)
                     /locus_tag="VGM72_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265427.1"
                     /translation="MGSIRKMIMTGAAVIVAGAAWHVESVAQENAGSGAGVVTVFDHG
                     KLDTSFAKALANGGTAPLWSRTSRYGTSDVSVHSRESTNSVCPPQGCSHKVYTAVVIV
                     VSGAATLEVGGTARAAADKFGGQRVQGGESHRISQGDVFIMPPDTMHWYKDVAAPFRY
                     IEVPVP"
     gene            complement(10151..10525)
                     /locus_tag="VGM72_08925"
     CDS             complement(10151..10525)
                     /locus_tag="VGM72_08925"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY4265428.1"
                     /translation="MRLIDTSAWIEWLIGSSTGRRVAAKIPDRAEWLVPTMVQLELAK
                     WLTRETGEDKADEVIAFTQTCVVADLTTNIALSAAELCRQHKLATADAIIYATALAHD
                     AELISCDKHFVKLPNVSYIAKG"
     gene            complement(10525..10740)
                     /locus_tag="VGM72_08930"
     CDS             complement(10525..10740)
                     /locus_tag="VGM72_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007427722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY4265429.1"
                     /translation="MRASTAKLSAKFQISIPKTVRAAKAWKAGQEFAFIPKGEGVLLV
                     PVPRTQELAGSVKGARPTRYRDRNDRF"
     gene            complement(10838..12148)
                     /locus_tag="VGM72_08935"
     CDS             complement(10838..12148)
                     /locus_tag="VGM72_08935"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY4265430.1"
                     /translation="MSHARVFLSTLAVFAVSASLAAAAQPQQNSTGVAAVVNEYVISN
                     YDLDQRTALFVATSGVRPTAENLPQIRGQVLRALEDEVMELQEANKRKINATKIEVDK
                     ALQNIAEDNKLTANQILATIGQAGVSANTFRQQISAQIIWQKLVAARYGADVTINDQQ
                     LDEAMDRLKQGSDKPQYLLSEIYMGVDKPEDEIAIRTSAEQLAQQTRQGAPFPTVASQ
                     FSQSPSAADGGDIGWVIQGQLADELDRALIDLKPGQIAGPIRAEGGYYVLLMRDRREP
                     AGTKAAAAPVAADPNAPVPLDRFLIPLPANPEAALKERAMALMVNIKAQARGCGDLPG
                     ISGQLQGTVLTPLGNMSPKDLEPALRDALAKTGPGDSVPPFFSGAGLELIMRCDKPVA
                     TVGTFELPTRDQLRQQLAAQQMSLYAKSYLRDLRREAVVYTTGR"
     gene            complement(12159..12356)
                     /locus_tag="VGM72_08940"
     CDS             complement(12159..12356)
                     /locus_tag="VGM72_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265431.1"
                     /translation="MRIILLAASVLLLAGCITPNMPARGASGQDALNARENDDTRTPD
                     VFRKPPRQFGKWGGYGYGERF"
     gene            12614..13828
                     /locus_tag="VGM72_08945"
     CDS             12614..13828
                     /locus_tag="VGM72_08945"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4265432.1"
                     /translation="MTKAEIHSAEGGAAVRPSRRSVLRTGLATAAGLAAAGALTATAP
                     ASAQITGVGAAEGTLPGRTAPHTLEGTSSTASPRQRLGIDIHAHYYPQEYLDAMAEGR
                     AYGGDYTETPQGYMLKAPGFSGGPFPAKFTDLKLRLADMDAQGIAVHALSLTRPMPYF
                     GPPDYALKLARAFNDGTSRAHLQYPDRFVGLMALPMMDKERSLDELMRASKLPGMRGV
                     YLGCNIEGRDFSDPEFLPVFLRIEALGLPIFLHPNGAVGGKRFEPFYLANILGNPFDT
                     TIAACHLIYGGVMDKCPNLQINLPHAGGAVPMLIGRMDHGYAVRRDVPALPRPPSEYM
                     RRFTYDTIGHNAGVVKFLISEVGADRVMLGSDYCLDMGYLRPVQDLDVLGLNPRDRDS
                     ILRGTASRLLKL"
     gene            complement(13831..15150)
                     /locus_tag="VGM72_08950"
     CDS             complement(13831..15150)
                     /locus_tag="VGM72_08950"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4265433.1"
                     /translation="MAPDPIDQALDHFRSGRYDESAQACLAILAGNSANTEINHLLGV
                     IRFHQGRPAEAIEFMRRAVTAPNASAEMHNNYGAVLNQMGETEEAIAAFNRALAMKPN
                     YADALNNLGVIYRDAKKTDAAIAAFRKAVDLSPDLLQAKANLRSAYRDVVPAWHFAMM
                     DDKRRNFAYEAAIRRAVPGKRVLDIGTGAGLLAMMAARNGAASVTTCETVSVIADRAR
                     EIVAQNGLAQRVKVIGKPSTELAAGRDFPERAEVLVTETFASALIGEGILATLEHAHQ
                     HLLTPDATVIPASGSIMGYLAGGDILKGMLFVDTIAGFDLSPFNDFAPPVLSTALDGV
                     AHEQLSDDVELLRFDFQQKSFPMAKHALQIRATKSGLCVGIAQWIKIALDSETSYENR
                     PAVNAEHNGHWVHMLHRFSRLVPVEPGDVVSIIVRHDRSQVNIDLVE"
     gene            15296..15853
                     /locus_tag="VGM72_08955"
     CDS             15296..15853
                     /locus_tag="VGM72_08955"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4265434.1"
                     /translation="MKLGHVVLNCSDVERSVRFYTQVLGLEISDVYPDDMVPGGMVFM
                     RFNEDHHGVALVGSMKGQSPNVELNHMAFEVATLDEVIVARNHLRKHGVKIDFEGRRR
                     AGVQIAVEFRDPDGHRLEIFWGLDRVAADGYIRPASEWKLARSLKQAVADPVAGQDTH
                     LKDSSILDENVEPQADMQSGGSSRW"
     gene            15860..16882
                     /locus_tag="VGM72_08960"
     CDS             15860..16882
                     /locus_tag="VGM72_08960"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY4265435.1"
                     /translation="MRFLPPLAAAFSLFAVSAWAADPAMIAAAKKEGSVVWYTTQIVD
                     QFARPAAAAFERLYPGIRVSLSRTNSATAAIKILNENRAHKNQSDVFDGTATVVPLKK
                     EGYVLKWIPDAAKTYPPQYKDPEGYWVATNIYVLTPAFNTRLIAPGQEPRTYSALLDP
                     KYRGKMVWGVSLSSSAAAGFIGTVLDDMGDEKGMAYLRQLQKQRIAGVDVSARQVLDQ
                     TIAGEYAIALQIFNHHAVISAKKGAPVKWIAMEPATGVLSVVSIPKAAPHPNAAKLFE
                     EFLVSNAGQRLYQAAEYLPADPDIPALDATLKPEGGHFRSRFFSPEETAEKMPRWKKI
                     YDEIFR"
     gene            16897..19029
                     /locus_tag="VGM72_08965"
     CDS             16897..19029
                     /locus_tag="VGM72_08965"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEY4265436.1"
                     /translation="MQASRAKVDALLNPKNVVILGATDRPGNWAQRVWRNLKRYDFPH
                     AVYPYNPARDAVWDTRCYRNFAELPEAPDHLVVLIPAAAVPDALADAAKAGARSATIM
                     TSGFDEAESLGAAELTMRLKRVIAETGLIVSGPNCLGNLHAPANLMTMPDDRPQTLSR
                     GPVAIVGQSGGIAMAIKRTLEERGVASGTVITSGNETGLTTADYIAYFAGAPEVKLIV
                     SYLESVRDADAFLAAARAARAAGKPVVVVKLGASRDGRAAALAHTGRLAGSMEAFDAI
                     AGAAGVMRVTNFDAVVEAVEYLAHAPLPRGAGLGAITFSGGLRGMLLDAAAAHGLKFA
                     DLAPATIGQLESLVSVGTTIGNPLDAGFAALTSQDAYLRSIETLLADPGIDTLLLQEE
                     LPRAAGTERKESNLRAVNAIAARAGKPIAFVTMISHGLTDYSRALRVELLNVAFLQEI
                     DKSLAATRSVIGYAATAKVRMSEHAPSARPTGKIAKLLNSHARALNEVESKALLKAYG
                     MPAPKEVLARTMRDAASAAKAIGFPVVAKAVSSALAHKSDAGAVKLGLTSVKDVRAAF
                     SQLEKIARAAHVTLDGVLIAQQVSDGIELVIGMHRDPEMGPVILFGAGGIALELHLDV
                     ALGPVPLDEAGALALIAKTRVAKRIEGYRGQPALDKKALVKALLGLSRFAVDVGPGLE
                     SVDVNPFLLRRRGGLALDALVVLSPHPK"
BASE COUNT         3549 a         6022 c         6065 g         3722 t
ORIGIN      
        1 tctggtgacg ctgcaggcct atgtctatcc gttcaccgaa atggtggtgc ggtaggcatt
       61 gcgtttttcc gtctctggaa cggatcgcgg cgatggaata atgtccacgg tcgtgccgtg
      121 acaaacattg gaaacacccc atgccgacca ccagcaagca ccccgagacc atcgcgctcc
      181 atgccggtta tcgcgcggat ccgtcgacgt cggccgtcgc ggtgcccatc tatcagacca
      241 cctcctatca gttcggctcg gccgaacatg cggcgaatct gttcgcgctg aaggagttcg
      301 gcaacatcta cacaagaatc atgaatccaa cccaagcggt gctggaggaa cgcatcgccg
      361 cgttggaagg gggcgcggcg gccttggcgc ttgcatccgg acagtcggcg tcgcttttcg
      421 cggttcagaa catctgtcac gcgggcgaca atttcgtctg ttccaccgac atttatggcg
      481 gcacctggaa tctgtttgcc aacacgatga agaccatggg aatcgaatgc cgtttcgtgg
      541 acccggccga ccccgagaat ttccgtcgcg ccaccgatgc gcgcacccgc tgctattatg
      601 gcgagacgct gcccaacccg aagctcgacg tgttccccat cgccgaagtc gcggccatcg
      661 ggcgtagcgc gggtgttccg ctgatcatgg acaacaccgc ggcgccggtt ctctgccggc
      721 ctatcgcgca cggtgcggcc atcaccatgc actcgaccac gaaatacatc ggcggccacg
      781 gtacatcgat cggcggcatc atcgtcgatg ccggaaattt cgattgggaa aaacacgccg
      841 agcgctttcc catgctcaat acgcccgacc ccagctatca cggcgcggtg tggactcagg
      901 cggtcaagcc gctcggcccc atcgcctaca tcatcaaagc gcgcgtgacg ttgctgcgcg
      961 atgtcggcgc ggcgatgtcg ccgttcaacg ccttcatgtt catccagggc ctggaaaccc
     1021 tttcgttgcg gatgcggcag cactgcgcca acgcggtggc ggtcgcgaaa tatctctccg
     1081 ggcattccaa ggtgacgcgg gtgatctttc cgggacatca gtcgggggaa agccggcgcc
     1141 gcgccgatgc ctatctcaag ggtggttacg gcggtcttgt cgggttcgag ctgaagggcg
     1201 ggagcgattc gggccgcgca ttcatcgatg cgctgacgat gttctaccat gtcgccaatc
     1261 tcggcgacgt ccgctcgctc gccatccatc ccgcgagcac gacccattcg cagctcacgt
     1321 ccgacgagca gatgcagacc ggcgtcaccg atggctatgt gcggctttgc gtcggcatcg
     1381 agcatatcga cgacatcatt gccgacctcg atcaggcgct cgctgcggcg taaccagtct
     1441 cgcggcgccc cgcgcggact aagataattt tcatcaattg cgcacgtatc ggtcgattgg
     1501 cgggaggggc caggattgag ttaacggcct ggaaagcgtg gcgcggccag attggcgcat
     1561 gtccacgctg tcgcccgcgc tcgatctgtt cctttatcag tttctgcgtt tcttcaatcc
     1621 gcgccagggc tattgctgga tctatcttgc aggcggatta ttttttgcgc tttgcgtcat
     1681 gctatggcgg cggcggaatc gttcccggat tcgcgcccgc ccgtttttgc ggcttctcgg
     1741 ctcgcgcgcg ctttggctgc accgctccac gcttctggac gtgaaattgt atttcctgca
     1801 cggcgtgctg gtgctgatcg cctatggcat gttcgaggcg acgagcgaga tctggcgcgc
     1861 gggtgctgcg tccgctctcg cagcgcttgc gggagcggag ccgcgcctgc atgcgccgca
     1921 cgcgatcgtg ggctctctca cgacgatgct tcaaatcatc gcgctcgatc tcggttactg
     1981 ggcgctgcac tattgcttcc acaaggttcc ggtcctgtgg gaattgcaca aggtccacca
     2041 ctccgcggaa gtcatgacgc ccctgtcgga gtggcgtcag catccgttcg aattcatcgc
     2101 ctttgccaat gtgctcacca tgacgaccgg cagcgtttac ggcgcgatgt cgtggttgtt
     2161 cggcgcggcg gcccaaccct tcacgctgtt tcagatcaac gcgcttttcg tgatgcatct
     2221 cctgaccttt caccatctgc gccattccgg tgtgtggatc gcagccaccg gcgggctggg
     2281 ccgcgtgttc cacagtccgg cgcatcatca gatccatcat tccaccgatc cgcggcattt
     2341 tgaccgcaat ctgggctatg cgctgtcgat ctgggactgg gccttcggca cgctttgcgt
     2401 tccggagcgc cctctcaagg tgcatttcgg ggtcgcgggc gaaccccctt accgcgggat
     2461 ggccgatacg ctcattcgtc cgcttgttgc ggcagcggcc aagctgcaat ttgctctcgt
     2521 tccaggacgc cggctctagc tccgcgaatt tgcggccgtc ccttcatttt tccgtttcct
     2581 ttcaagcaga cggatgggct ttatgacaat tcgattccaa atccgcattt catgttgaac
     2641 actttgttac gaaatgcggg aaatactgga tgggcaaaga ggtccctccc atgtccgctc
     2701 cgcactccaa actcgtgatg ctcgccgaca tggcagccga ggcatcgggc gatgcgcgcc
     2761 gcgaattgct gcggcagatc gccgatagtt tccgtcccga gaataccgcc ttgaatcgga
     2821 tcaatcgtgc ggacctcgac aagatcatga gcgcgatcat ctgcgaattc accatcgcga
     2881 tccgtaccga actgtcacgc tatatcgccg ccagtccttt gcccttcaat gccacggcga
     2941 ggaacctggc cctggacgaa atttccgttg cccggccggt gctcgaacgt tcgactgcac
     3001 tgacggacca agacctgctt gaggtcatcg ctcaaaaatc ccaggcgcat atgacggccg
     3061 tggccaagcg cgcgcatgtc agtgaggacg tctgcgaggc tcttgtcgaa tatggtgagg
     3121 atcgtgtcgt cgcctcgctt ctggacaatg cgggcgcgaa aatcgcttat ggcacctatg
     3181 agaaggtcgc ggcccgcgcc gaaaccagcg ttctgctgca agggcctttc attcagcgca
     3241 gcacggtgcc gctcgatctc ctgaacgatc tgtatttcaa ggtcgaaggc gatctgcgcc
     3301 gtcaggtgct tgagcatttc gagaccgcgt ccaagcagga tctgtcggtg gcccttgtgt
     3361 tgagccgcag acgctggttg gtgaaatatg gcgggctgcc gaagaactat gaaactctca
     3421 gcagccggat cgacgaattt catcgccgcg gcgaactcaa gccggccatg ctggtcggct
     3481 acctgcgcag ccatctgcac acggagttca aactgggctt cgcgaaactg acgggcaccg
     3541 actacgaact cgtcaatcgg ctggtcgagc gcaacgacct tgatggcgtg gcgatcctcg
     3601 cgcgcgcggg aaatttcgac agagcctttt tcgtgacggt cgccatgatg ctctcgggcg
     3661 gcggcaaccg gatggccgac gcggaatcgt tcggcgcgca atatgaggcg attagcgtcg
     3721 aacaggcgtc gcgcgtggtt cgcatgtggc gggtgggacc cgaaagcgag gcccgccaac
     3781 cggttcgcct ggcgtcttga tcgcgggccg gccagggtcc gccgggttac tccgcgggag
     3841 cgggacgcgg cttggccatc gatacgcctt cgtccgtgaa ctggcgttcg ggatgcatgg
     3901 cgaacgtcat gagagcgccc accaagagca cgccgatcga catgatgaag ggcagatacc
     3961 aattgccggt ccggtcgatc acatagcccg cgatggcagg cgacaggatc gcggccaggg
     4021 ccgagcctgt gttcatcaac cccgccgcgg tgccggaata tttcggcgcg atgtccatgg
     4081 gaatggccca catgggaccg atcaccagct cctcgaaaaa gaacccgctg gtgagacaga
     4141 gcgcgacgat gttgatgtcg tggatataca ggatcggcac cagcgacagc agcgctccgg
     4201 tgaagccgag cgcgcagatc gacagccgcg ccagcctgac atttttggtt ctgtggtaga
     4261 tcgcatccga gaacaccccg ccaagcgtgt cgccaaccac ccctgccagg aacacgccgg
     4321 tcgcgaagat ggccgattgc ttgatgtcga ggctgtaggt gttcttgaag aacagcggca
     4381 gccaattgag atacagccag agactccagc catagcagaa ataggtgatt gtcaccggcc
     4441 acatgcgtcc gatcaaagga ccccagggta cgatgggctt gggtcctttg gggcggtgcg
     4501 gcagcagcgc gagttcggcc tgcgtgatcg agggatgttc tttcgggtcg tcgcggaaat
     4561 accacagcca aacgaacacc cagatgaagc cgaatgcgcc gaccgcgatg aacgagcccc
     4621 gccactcgat cacggacatc aaagcggcga tcagaagcgg cgtggccgcg ttgcccagcc
     4681 gcgcaaaggc atgcgtgatg ccctgggcga agccacgttt ccttgcctcc acccaatgct
     4741 gcatggcgcg ggtggcggtc ggcagcgtgg cgccgacgcc gaagcccatc agcacgcgaa
     4801 gcgcaaacag cgaatagaaa tcgaaggcga ggctggtcag gatcgtggcg cccgcccaca
     4861 cgatgccgct gacgaatagc gtgcgccggg gcccgaaatg atcggcggtc cagccgccga
     4921 tgatctggaa cagcagatag ggaatcccat aggcggagaa gatcaggccg agctgcgtat
     4981 tggaaaggcc gaattcctgc tggatcggac cgccggcggt gttgacgttc tgccgcacga
     5041 tataggtgat gaaatacatc gcgcagagca ggacgagaac gatattggta gtcttgaaaa
     5101 tgcgcggcat cgcgtctttc tccccgcttc cgtcccggct gcctattcta ttcggcggcg
     5161 ctttccatcg gcgtcttggc ggtctttgcg agataatcca tcgccgcctg tgcgccgccc
     5221 ttcttgtgcg gcacgcccgc cagttccagg cccatctcga cgccggatag cgcgcccagg
     5281 atcgtcagat cgttggtgtc gccgagatga ccgatgcgga acaccttgcc ggcgagcttg
     5341 gaaaggccgg cgccgagcga catgtcgaaa ttttccagca cgattttgcg gaacgcatcc
     5401 gaatcatggc ctgccggcat caggatggcg gtcagcaccg gcgagtagca cttcgggtcg
     5461 gagcagagga tttcgaggcc ccaggcccgc accgcgcgcc gggttgcttc ggcatggcgt
     5521 ttatggcgcg cgaagacgcg gtccaggcct tcttcatgca gcatgtcgac ggcttcggcg
     5581 agtgcgtaga gcagattggt ggccggcgtg taggggaaga agccgctggc gttgggaccg
     5641 gccatgtcct cccaggaaaa aaaggatttc ggcagcttcc cggccttggc cgccttcatc
     5701 gccttttcgc tgacggcggt gaaaccgagg ccggggggca gcatcagccc cttctgcgag
     5761 cccgcgacgg tgacatccgc cccccattcg tcatggcggt aatcgaccga ggcgagcgag
     5821 gagatggtgt cgaccagaag cagtgccggg tgccccgccg catcgagcgc cttgcgcaca
     5881 tcggcgatgg gcgaggtgac gccggtcgag gtctcgttgt ggacgacgca gaccgccttg
     5941 atctcgcgat tggtgtcggc ccgcaatctt tcttcgatct tggccggatc gacgcccgtg
     6001 cgccaatcgg attcgatgaa ttccggcttc acccccagtt tcagcgccat ggtcttccac
     6061 agcgtcgcga aatggcccgt ctcgtacatc aggacgcggt cgccgggcga cagcagattg
     6121 gtcagcgacg cctcccaggc cccggtaccg gaggagggat agatgaagac cgcccccctg
     6181 gtcttgaaaa tcgtcttgat cccggacaaa acccgctgcc cgagctgctg gaaatcgggt
     6241 ccgcgatgat cgatcacggc acggtccatc gcccgcaaga tgcggtctgg aaccggggtc
     6301 ggccccggga tttgcaggaa atgacggccg gcgatgcggg gggtgttggt cggcatgggt
     6361 gctcgaaagt tggggctttt gggggctttt ctttcacacg tccggccctt gtacacaagg
     6421 gcgggcaact tcataagagg cacatgacca tcgtcgcgag ccccggcctg gaaaggcgcc
     6481 tgaaatccga gaacaccggc gaaatccttt tcgaccgctt cacgcggggg cgttacgcca
     6541 ccgatgcctc ctcctatcag atgatgccgc tgggcgtggt tgtgccgcgc agcgtggacg
     6601 aagcggtgcg cgccatcgcg attgcccgcg ccgagggcgt cagcgtggtg ccccggggcg
     6661 gcggcacctc gcaatgtggc cagacgatta accattccct cgtcgtggac tgctcgaaat
     6721 atctgaacaa aatcgtctcg ctcgatggga atgcgcggac ctgcgtcgtg cagcccggca
     6781 tcgtgctgga cgagctgaac cggcaattaa agccgcatgg cctctggttc gcggtcgatc
     6841 cctccaccgc gtcgcgcgcc acgctgggcg gcatgaccgg caataattcc tgcggcacgc
     6901 ggtctttgcg ctacggcacc acgcgcgaca acatcatcgc cgtgaacgcg gtgcttccca
     6961 atggcgcgcc ggcgcgattt gcggaagttt cgcgcgatct gtccgaactg ccggcggatt
     7021 cggcgctgcg tcccatcgcg cgcgccttgc tggcgctcgg cgagcgcgag gcggccgaga
     7081 tcgaggcgcg ctttcccaag gtccagcgcc gcgtcggcgg ctacaatctc gatgcgctgg
     7141 tgcccaagaa caccaagacc aatctcgctc acattctcgt ggggtcggaa ggcacgctcg
     7201 ctttctcgac cgggatcgag ctgaagctct cgcctctcct gggtcgccgg gtgatggggg
     7261 tctgtcattt cggccagttc tacgaagcga tgaacgccgc ccagcatctc gtcacgctcg
     7321 gccccatcgc ggtcgaggca atcgacaaga ccatgctgga tctggcgcgc gcgattccgc
     7381 tgtttcgcga cgtgatcccg aaattcaccc gcgaagatcc cgaagccctg ctgatggtgg
     7441 agttcgacga gggcgacgag gagaacgaac gccgcatgaa gcggctcgtc gagatgatgg
     7501 gcgatctcgg attttcctgg gacaaaatcg gcgccaaatg gggcggcgtg gtggaggtgc
     7561 gcgacgcggg tttgcaggcc gcgatcgccg aggtccgcgc gtccggcctc aacatcatga
     7621 tgagcatgaa ggatgagcgc aaacccgtgt ccttcatcga ggattgcgcg gtgccgctcg
     7681 tgcatctcgc cgaatacacc gaccgcctga cgcaagtgtt cgagaaatac ggcaccaaag
     7741 gcacctggta tgcgcacgcc tccgtcggct gcctgcatgt gcgcccggtg ctgaatttga
     7801 agctcgaaaa agacctgaag gcgatgcgcg ccattgccga ggaagccttc gagctggtga
     7861 agaaattcaa gggctcgcat tccggcgaac atggcgacgg catcgtgcgc tccgaatttc
     7921 acgagatgat gttcggcacg gaattggtgc gcgcgttcgg cgaggtgaag gcgctgttcg
     7981 atcccgagaa attcttcaat cccggcaaga tcgtgggcgc gcccaaattc gacgatcctg
     8041 attatctgcg tttcggcccg gcctatgaga tttccgcttt caagccggcg ctggactggt
     8101 cggaatttcc cggcggtgca tcgggttttc agggcgccgc cgagatgtgc aacaacaatg
     8161 gcgcctgccg caagaccgaa ggcggcgcca tgtgtccctc ctaccgcgcg acccgcaacg
     8221 aacgcgatgt cacgcgcggc cgcgccaaca gtttgcggct cgcgctgtcg gggcagcttg
     8281 gcgcggatgc gctcgcctcg gacgagatga tggagacgtt gaagctttgc gtctcctgca
     8341 aggcctgcag gcgcgaatgt ccaaccggcg tggacatggc gaaattcaag atcgaggtgc
     8401 tagccgcgcg ggccgccaaa catggcgtct ccttgcgcga caaattgatc ggctatctgc
     8461 cgcgctatgc gccatggggc gcgcgcttcg cgtggttgct gaatctacgc gaccagatcc
     8521 cgggcgcggc cgctttaagc gaacccatca tcggctttag cgccaaacgc tcgctgccga
     8581 aatggcgcac gcattacttc aatcctgcgg caaccgtcat cgaatccacc ctccccctag
     8641 tgggtgaagt cgtgctgttc gcggacactt tcaaccgcta tcacgaatcc gaaaatctgg
     8701 acgcggcatt ggccgtgctc accggtgccg gttatcgcgt gcatctgccg aaggccgtga
     8761 acggcaatgc acggccgctc tgctgcgggc gcactttcct gacggcgggg cttgtggacg
     8821 aagcgcgcgc ggaaaatgcg cgcagtgcgg cggcgctgct ccccttcgcc aaacgcggca
     8881 tcccgattgt tggactcgaa ccctcctgca ttttcacctt ccgcgacgaa gcgccggcca
     8941 tcgcgccggg tgaggattcc aagctgctcg ccaagagcac gttcctgttc gaggaattct
     9001 tggcacgcga aatcgacgcc gggcgcttca atccgaagct ggcgcctatt ggcgcaaatg
     9061 cgctgctgca cggccattgc catcagaagg cgttcaacgt catgggcccg gtgcagaagg
     9121 tgctttcgaa agtgccgggg ctgaaggtcg agctgatcga atcctcctgc tgcggcatgg
     9181 cgggcgcatt cggttatcag tccgagacca tcgatgtgtc gctgaagatg gcggagctgt
     9241 cgctcttgcc cgccgtgcgc aaagcgagtg ccgatgcgat tgtcgtagcc gacggcactt
     9301 catgccggca tcagatccac gacggcacgg gacgcaccgc gctccacgtc gccgccgtcc
     9361 tcgccaaaag catggccgcg gcaaacgcgt agagcctgct gccggagatc ggaattatgg
     9421 aaccggcacc tcgatataac ggaagggcgc cgccacatcc ttgtaccagt gcatcgtgtc
     9481 cggaggcatg atgaacacat cgccttggct gatacgatgc gactcgccgc cttgcacccg
     9541 ctggcccccg aatttgtcgg ccgccgctct ggctgttcct cccacctcca gcgtcgccgc
     9601 cccgcttacc acaatcacca ccgccgtata gaccttatgg ctgcatccct gtggcgggca
     9661 tacggaattc gtggactccc ggctgtgcac gctcacatcc gatgtcccat agcgggatgt
     9721 gcggctccag agcggcgcgg tgcctccgtt tgccagggcc ttggcaaagg atgtgtccag
     9781 ctttccgtgg tcgaacacgg taacaacgcc agctccggaa cccgcattct cctgcgcgac
     9841 gctttcgacg tgccacgccg ctcccgccac tatgaccgca gcaccggtca tgatcatctt
     9901 tcggatgctc cccatcgcga cctcctttag aacttcccga gtagcccgag gatattgtca
     9961 aagcgagcgt gcctcaaccg taccgccatc atgtcagggc tcatgacccg aggacatgag
    10021 gccggccatg tgacatcggg aaggcgcgct tagcgcccgg tcatgtagac cacgggcttc
    10081 gcggcgaagg tcgcgcagat agctcttggc atagagcgac atctgttccg gaagccaatt
    10141 cccgtcaatt ctatcctttg gcgatatagg acacgttcgg caacttcacg aaatgtttgt
    10201 cgcaggagat aagctccgca tcatgcgcca atgccgtagc atagataata gcatcggccg
    10261 tggccaattt gtgctgcctg cacagttccg ccgccgaaag cgcgatgttt gtcgtcaaat
    10321 cggcgacgac gcaggtttgc gtgaaggcga tcacttcgtc ggctttgtcc tctccagttt
    10381 ctcgcgtcag ccatttggcg agttcgagtt gcaccatggt tggcacaagc cattccgcgc
    10441 gatccgggat tttggccgcc acccgccttc ccgtgcttga accgatcagc cactcaatcc
    10501 aggccgacgt gtcgatgagc cgcatcagaa gcggtcgtta cggtcgcggt atcgcgtagg
    10561 ccgcgcgccc ttgaccgagc ccgcaagctc ttgcgtccta ggaactggaa caagcagtac
    10621 gccttcaccc ttcgggatga aggcgaattc ctgccccgcc ttccaagctt ttgcggcacg
    10681 tacggtcttg ggaatggaga tttgaaattt ggcggaaagt tttgccgtac tggcgcgcat
    10741 tacgacatcc tgatcgatca tcatatcgta agacgattag caccaaaggt cacgagatgt
    10801 caccaaccct ccctcaaggg agggctggaa ggcgcgccta ccgcccggtc gtgtagacca
    10861 cggcttcgcg gcgaaggtcg cgcagatagc tcttggcata gagcgacatt tgttgcgcgg
    10921 cgagctgttg gcgcaattga tcgcgcgtcg gcaattcgaa ggtgcccacc gtcgccacgg
    10981 gcttgtcgca gcgcatgatc agctccaatc cggcgcctga aaagaacggc ggcaccgaat
    11041 cgccggggcc ggtctttgcc agggcgtcgc gcaatgccgg ttcgagatcc ttcggactca
    11101 tattgccgag cggcgtcagc acggtgccct gaagctggcc ggaaatgccg ggaagatcgc
    11161 cacagccgcg cgcctgggcc ttgatattga ccatcagcgc catggcgcgc tccttcagcg
    11221 ccgcctcggg atttgccggc aggggaatca ggaagcggtc gagcggcacg ggcgcgttcg
    11281 gatcggccgc gaccggcgcc gccgcggctt tggttccggc gggctcccgg cggtcgcgca
    11341 tcagaagcac gtaatagccg ccttcggccc ggatcggtcc ggcgatctgc cccggcttaa
    11401 gatcgattag cgcgcggtcg agttcgtccg ccaattggcc ctggatcacc catccgatat
    11461 ccccgccatc ggccgccgat ggcgactggc tgaactggct ggcgacggtc gggaacgggg
    11521 caccctgcct tgtctgttgg gcgagttgct cggcgctggt gcgaatggcg atctcgtcct
    11581 cgggtttatc gacacccata tagatttccg acagcaggta ttggggcttg tccgaaccct
    11641 gcttcagccg gtccatggct tcgtccaatt gctgatcgtt gatcgtgacg tcggcgccgt
    11701 aacgcgcggc aaccagtttc tgccagatga tctgggcgct gatctgctgg cggaaggtgt
    11761 tggcgctgac gccggcctga ccgatggtgg cgaggatctg attggcggtg agcttgttgt
    11821 cctcggcgat gttctgcagc gccttgtcga cttcgatttt ggtcgcgttg atctttcgct
    11881 tgttcgcctc ctgcagctcc atgacttcgt cttccagggc gcgcagaacc tggccgcgga
    11941 tttgcggcag gttttccgcg gtcggccgca cgcccgacgt ggcgacgaaa agcgccgtgc
    12001 gctgatctag atcgtaattg gagatgacgt attcgttgac cacggcggcc acgccggtgg
    12061 aattctgctg tggctgtgcg gcggccgcca gggacgcaga gaccgcgaac accgccagcg
    12121 tactcaggaa cacacgcgcg tgggacaagg ggaaactcct aaaagcgctc gccataacca
    12181 tagccgcccc atttgccgaa ttgtctcggc ggcttgcgga agacatccgg ggtgcgggtg
    12241 tcgtcattct cgcgcgcatt cagggcgtcc tgccccgatg cgccacgggc aggcatgttg
    12301 ggggtgatgc agcccgccag aagaaggacg ctcgccgcca gcaagatgat gcgcatggtg
    12361 tcctccctca aaatgtcttc ccgcaaatgc gaaacgccat cataggccgg gcagggcgct
    12421 cgggccaaat ttttcccgcc tgcccgcagg gtggtagact gtccagctga ggtccgttgg
    12481 gcttcaccgg cgcatggggc aattgcaccg gaaaaatcag gacggtttct gatatttttg
    12541 gcggaaaacc ggcaaaaccc cactccatcc ggcaaaataa gggagggggg ggtacgagaa
    12601 aaggagcgag cgcatgacca aggccgaaat ccattccgcg gaaggcggtg cagcggttcg
    12661 tccctcgcgc cgcagcgtgc tgcggacggg cctcgcgacg gcggcagggc tggccgccgc
    12721 cggtgctctc accgccacgg cgccggcatc ggcgcaaatc accggagtcg gcgccgccga
    12781 aggcaccttg cccgggcgta ccgcgcccca cacgctggaa ggcacctcgt ccacggcgag
    12841 cccgcgccag cgtctgggca tcgacatcca cgcccattac tacccgcaag aatatctgga
    12901 tgcgatggcg gagggccgcg cctacggcgg cgattataca gagaccccgc aaggctacat
    12961 gctgaaggcg ccgggatttt ccggcgggcc tttcccggcg aaattcaccg atttgaaact
    13021 gcgtctcgcc gatatggacg cgcaagggat cgccgtccac gcgctgtccc tgacgcggcc
    13081 catgccttat ttcggtcccc ccgattatgc gctgaagctg gcgcgcgcct tcaatgacgg
    13141 gacaagtcgc gcccatcttc agtacccgga tcgtttcgtg gggctgatgg cgcttcccat
    13201 gatggacaag gagcgttcgt tggacgagct gatgcgcgcc tccaagcttc cgggcatgcg
    13261 cggggtttat ctcggctgca atatcgaggg tcgcgatttt tccgatcccg aattcctgcc
    13321 cgtcttcctg cgcatcgagg cgctgggcct gcctattttc ctgcatccca atggcgccgt
    13381 cggcggcaag cgtttcgagc ccttctatct cgccaacatt ctcggcaatc ccttcgacac
    13441 cacaatcgcg gcctgccatc tgatctatgg cggggtgatg gacaaatgtc cgaacctcca
    13501 gatcaatctg ccccatgcgg gcggcgccgt cccgatgctg atcgggcgga tggatcacgg
    13561 ctacgcggtc cggcgcgatg tccccgcttt gccgcggccg ccatcggaat atatgcgccg
    13621 tttcacctat gacacgatcg gtcataatgc gggggtggtg aaattcctca tctccgaagt
    13681 tggcgcggac cgcgtgatgc tgggcagtga ttactgcctc gacatgggct atctgcgccc
    13741 ggtccaggat ctggacgtgc tcgggctcaa cccgcgcgac cgcgattcta tcctgcgcgg
    13801 aacggcgtcg cggcttctga agctgtagct ttattccacc agatcgatat tcacctgcga
    13861 gcggtcgtgc cgcacgatga tggacacgac atcgcccggc tcgacgggga ccaatcgcga
    13921 gaagcgatgc agcatatgca cccaatgtcc gttgtgctcg gcattgacgg cgggccggtt
    13981 ctcatagctg gtctcggaat cgagcgcgat tttgatccat tgcgcgatac cgacgcagag
    14041 cccggatttg gtggcccgga tttgcaaagc gtgctttgcc atcgggaagc ttttttgctg
    14101 aaagtcgaac cgcagcaatt ccacatcgtc cgacaattgc tcatgggcga ccccgtcgag
    14161 agccgtactg agaaccggcg gcgcgaaatc gttgaacggc gacagatcga acccggcgat
    14221 ggtgtcgacg aacagcatgc ccttcagaat atccccgccg gccagatagc ccatgatgga
    14281 gcccgaggcc gggatcaccg tcgcgtcggg ggtcaagaga tgctgatggg cgtgttcgag
    14341 cgtggcgagg attccttcgc cgatgagcgc gcttgcgaag gtctcggtga cgagcacctc
    14401 cgcgcgttcg ggaaaatcac ggcccgcggc gagttcggtc gagggcttgc cgatgacctt
    14461 gacgcgctgg gccaacccgt tttgcgccac gatctcgcgg gcgcggtcgg cgatcacgct
    14521 gaccgtctcg caggtcgtga cgctggcggc cccgttccgc gccgccatca tcgcaagcag
    14581 ccccgcgccg gtgccgatat cgagcacgcg cttgcccggc accgcgcgcc ggatcgcggc
    14641 ctcataggcg aaattgcgtc tcttgtcgtc catcatggcg aagtgccagg ccggcaccac
    14701 gtcgcgatag gccgagcgca gattggcctt ggcctggagc agatcggggc tgagatcgac
    14761 cgccttccgg aacgcggcga tggcggcgtc ggtttttttc gcgtcgcgat agatgacgcc
    14821 cagattgttg agggcatcgg cgtaattggg tttcatggcg agcgcccggt tgaaggcggc
    14881 gatggcctcc tcggtctctc ccatctggtt gagcacggcg ccgtaattgt tgtgcatttc
    14941 cgccgatgcg ttgggcgcgg tgacggcgcg ccgcatgaat tcgatggcct ccgcaggccg
    15001 gccctgatgg aaacggatga cgcccagaag atgattgatc tcggtgttgg cgctgttgcc
    15061 ggcgagaatg gccagacagg cttgcgccga ttcgtcatag cggccggacc ggaaatgatc
    15121 gagcgcctga tctatggggt cgggggccat ggcgtctcgt tcttttgcga gcgggacatt
    15181 aaaaccttgc gcggctacaa tgcaaagccg cttttaaggg agccataccg gcatgggaat
    15241 cgaccccgat tcaatacgaa aatacgcacg gcccaaagat acgccgttcg atattgtgaa
    15301 gctcgggcac gtggtgctca actgctccga tgtcgaacga tcggtgcggt tttacacgca
    15361 ggtgctgggc ctggaaatat cggacgtcta ccccgacgac atggtgccgg gcggcatggt
    15421 gttcatgcgt ttcaacgagg atcatcacgg cgtggcgctg gtcggttcga tgaagggcca
    15481 atcgcccaat gtcgaactca accatatggc cttcgaagtc gcgacgctgg atgaggtgat
    15541 cgtggcgcgc aatcatttgc gcaagcacgg tgtgaaaatc gatttcgaag gccgccgccg
    15601 ggccggcgtg cagatcgcgg tcgaattccg cgatcccgat ggacaccggc tggaaatctt
    15661 ttggggcctg gatcgcgtcg ccgcggatgg ctatatccgc cccgcctcgg aatggaagct
    15721 ggcgcgcagc ctgaagcagg ccgtggccga tccggtcgcc gggcaggaca cgcacctgaa
    15781 agactcatcc attctggacg aaaatgtcga gccgcaagcg gatatgcaat ccggcggcag
    15841 ttcgcgctgg tgagccgcca tgcgctttct tccgccgctc gcggccgcgt tttcgctttt
    15901 cgccgtctcg gcgtgggccg ccgatcccgc gatgatcgcg gccgcgaaga aggaaggcag
    15961 cgtcgtctgg tacaccaccc agatcgtgga tcaattcgca cgccccgcgg cggcggcgtt
    16021 cgaaagactg tatcccggca tcagggtaag cctgtcgcgc accaattccg cgaccgccgc
    16081 gatcaagatc ctgaacgaga accgggcaca caagaatcaa tccgatgtct tcgacggcac
    16141 ggccacggtg gtgccgctga aaaaggaagg ctacgtcctc aaatggattc cggacgccgc
    16201 gaagacctat ccgccgcaat ataaggaccc ggaaggctat tgggtcgcga ccaacatcta
    16261 tgtgctgacg cccgcgttca ataccaggct gatcgccccc ggccaggagc cccgaaccta
    16321 ttccgccctg ctcgatccca aataccgcgg caagatggtg tggggcgtca gcctgtcgag
    16381 ttcggccgcg gcgggcttca tcgggacggt gctcgatgac atgggcgacg agaaaggcat
    16441 ggcgtatctg cgccaattgc agaagcagcg tatcgcgggc gtcgacgtgt cggcccgtca
    16501 ggtgctggac cagaccattg ccggcgaata cgcgattgcg ctgcagatct tcaaccatca
    16561 tgcggtgatc agcgccaaga agggcgcgcc cgtgaaatgg atcgccatgg agcccgcaac
    16621 cggcgtgctg tcggtggtct ccatcccgaa ggcggcaccc catcccaatg ccgccaaact
    16681 gttcgaggaa ttcctggtct caaacgcggg ccaacggctt tatcaggcgg cggaatatct
    16741 gcccgccgat ccggacatac ccgccctgga cgccacattg aaacccgagg gcggacattt
    16801 tcgctcgcgc ttcttttcgc cggaagaaac ggccgagaaa atgccccgct ggaaaaaaat
    16861 ctatgacgag atattccgct gatgggagag ggggaattgc aggcatcgcg cgccaaggtc
    16921 gatgcgctgc tcaatccgaa gaatgtcgtc atcctcggcg ccaccgaccg ccccggcaat
    16981 tgggctcagc gcgtctggcg taatctgaag cgctatgact ttccccatgc cgtttatccc
    17041 tacaatcccg cccgcgacgc ggtctgggac acgcgctgct atcgcaattt cgcggagctg
    17101 ccggaagcgc ccgatcatct ggtcgtgctg atcccggcgg ccgccgtacc ggacgcgctg
    17161 gccgatgcgg cgaaagccgg cgcccgcagt gccacaatca tgacatcggg cttcgacgaa
    17221 gcggaaagcc tgggcgccgc cgaactgacg atgcgcctca agcgcgtgat cgccgagaca
    17281 ggccttatcg tgtccggtcc gaattgcctc ggcaatctgc acgcgccggc caatctgatg
    17341 accatgccgg acgaccggcc gcagacgctt tcgcgcggac cggtcgccat cgtcggccaa
    17401 tccggcggca tcgccatggc gatcaagcgc acgctcgaag agcgcggcgt ggcgagcgga
    17461 accgtcatta cctcgggcaa tgagaccggg cttaccaccg ccgattacat cgcctatttc
    17521 gcgggcgcgc ccgaggtgaa attgatcgtc tcctatctcg aatcggtgcg cgatgcggat
    17581 gcgttcctgg ccgccgcccg cgccgcgcgg gccgcgggca agccggtcgt cgtggtgaaa
    17641 ctgggcgcgt ccagggacgg ccgcgcggcc gcgcttgccc ataccggccg gctggcgggg
    17701 tccatggagg cgttcgacgc cattgccggt gcggccggcg tgatgcgggt gaccaatttc
    17761 gatgccgtgg tggaggcggt ggaatatctc gcgcatgcgc ccttgccgcg tggggcgggg
    17821 cttggcgcca tcaccttctc ggggggcttg cgcggtatgc tgctcgatgc ggcggcggcc
    17881 catgggctga aattcgcaga cctcgcaccg gccaccatcg ggcagttgga atcgctcgtc
    17941 tcggtcggca caacaatcgg caatccgctg gatgcgggct tcgcggcgct caccagccag
    18001 gacgcctatt tgcgctcaat cgaaacgctg ctcgcagatc ccggcatcga cacgctgctc
    18061 ctgcaggaag aattgccgcg cgccgccgga accgagcgca aggaatccaa tctgcgcgcg
    18121 gtcaacgcga tcgctgcgcg ggcgggcaag ccgattgcct tcgtgaccat gatttcgcat
    18181 ggcctcaccg attatagccg cgcgctacgc gtggaattgc tgaatgtggc gttcctgcag
    18241 gagatcgaca aatcgctggc cgcgacgcgt tcggtgatcg gctatgccgc gacggcgaag
    18301 gtgcggatgt ccgaacatgc gccttcggct cggcccacag gaaaaatcgc caagctgctc
    18361 aattcccacg cgcgtgcttt gaacgaagtg gaatcgaagg cgctgctaaa agcctatggc
    18421 atgccagcgc ccaaagaggt tctcgcgcga accatgcgcg acgccgcatc ggccgcgaaa
    18481 gccatcggct ttcccgtggt cgcgaaggcc gtcagctccg cgctggcgca caaatccgac
    18541 gccggcgcgg tcaagctcgg cctcacttcg gtgaaggatg tgcgtgccgc cttttctcaa
    18601 ctcgaaaaaa tcgcgcgggc cgcgcatgtg acgctcgatg gcgtcctgat cgcacagcag
    18661 gtctccgacg gaatcgagct tgtgatcggc atgcatcgcg atcccgagat gggtccggtg
    18721 atcctgttcg gcgccggcgg tatcgcattg gaactgcacc tcgatgtggc gcttggcccg
    18781 gtgccgctgg atgaggcggg cgcgctcgcg ctcattgcca agacgcgcgt ggcgaagcgg
    18841 atcgaaggct atcgtgggca gccggcgctg gataagaaag ctctggtcaa ggcgctgctc
    18901 ggactctcgc gatttgcggt tgatgtgggg ccgggactcg aatccgtcga cgtgaatccg
    18961 tttttgctca ggcggcgcgg cggcctcgcg ctcgacgcgc tcgtggtcct ctccccccac
    19021 cccaaataaa agatagtcgg atttagatat caaaagcgga cattcgacca gcgattggga
    19081 aggcccgcta ttggccagag aaagacttcc caatgctccg tgtgaaagcc gacgctcaca
    19141 cgcgccagct tgcaatgcgc attaggcgcg gaggggcgtt ttcctaagcg gtcttgaaca
    19201 tcagcttact ggccttggcc ttgacccgca gcacccgggt cgacgaggcg atgtagaggt
    19261 agccgtccgc cccgaactcg cagttggcga taccctggtc ggacgagatg aagcccaaat
    19321 gcgcgccctc ggggctgaag acggtgacgc cctcgcgg
//
LOCUS       DASYEG010000199         4657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2093911, whole genome shotgun sequence.
ACCESSION   DASYEG010000199 DASYEG010000000
VERSION     DASYEG010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2093911"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(442..1548)
                     /locus_tag="VGM72_08970"
     CDS             complement(442..1548)
                     /locus_tag="VGM72_08970"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265437.1"
                     /translation="MARFGRMVALAGFMACAVTGFAYAEEPKFQIDTSWPKPLPNNWI
                     FGQIGGIFVDKDDNVWITQRPRTVNKRDRRMQDGDHSICCRTAPSVIEFNQAGDVIRS
                     WGGPETAKGFDWPANEHGIFLDYKNNIWFGGNGEKDGMIVKFTIDGKFEMMIGKSAPQ
                     TNSLDQTRVGRAADMVVDPATNELYVADGYFNHRVVVFDADTGKFKRTWGAYGKPPTD
                     EKITYDPKVLPQQFNNPVHGIRITNDGEVVVSDRANNRIQIFKKDGTFIREIALLRDS
                     TAPGVTGSTVFWPDKGQSIWFTSDDPNGQIHIFQRSDGKILGSFGRVGRGPGEFENLH
                     NIAIDSKGNIYSAEVQGQRVQRFKYVSGLPPIGK"
     gene            complement(1651..2997)
                     /locus_tag="VGM72_08975"
     CDS             complement(1651..2997)
                     /locus_tag="VGM72_08975"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY4265438.1"
                     /translation="MTLRSLIFAFLVLFVPAAANAITVKAVAGPTGVETWLSEERALP
                     MIAVNISFPAGSAYDPADKPGLANMVASLLDEGAGDLASDAFKQALESRAIRFQATAD
                     RDYIVVTLTTLKENADEAFRLAGLAFAHPRFEPEAVERIRVALLASLKQEDEDPSRAA
                     VKAWYAAYFGAHPYGRPPAGTAAGIAAIRADDIRAFAAEHLVRGGIKMAVSGDVTEVQ
                     LKKYLQALFTPLPVKSGLPVSKPVDAGRPGTRNVVRNEAAPVAIFGFAGPMRADPDFI
                     PTFVTNYILGGGGFSARLMDEVRDKRGLTYGISTQLNDFRSASVIVGSVQSDKTKILT
                     ALDVTKSEMARFAKDGAAAKELDDAKTYLTGSFPLGLDSNAKIARTLNAYQRSGLAAD
                     YVVKRNAMIQAVTLAQVNAMAKKYFDPARLVVVIAGTPAAPEAATKPPAPTGNAAR"
     gene            complement(2994..4388)
                     /locus_tag="VGM72_08980"
     CDS             complement(2994..4388)
                     /locus_tag="VGM72_08980"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY4265439.1"
                     /translation="MRAVRNALAGIALLGAGVGALAVTGYFVRHADASAPETAPVKAA
                     NKATQFMLKNGLQLVVIPDHRAPVVTHMVWYHVGGADDPPGLSGEAHLFEHLMFKGTK
                     TVPNGELSKIIARNGGQDNAQTSHDFTVYFQRIAKDRLPIVMGLEADRMVNLDLSEPN
                     VTTERDVVLEERHLRVDSEPQALAQEQMAAALHLSHPYGRPVIGWEKEIRSIGRVQAN
                     DFYQHHYAPNNAIVMVVGDVTPDEVRKIAEDKYGAVPSRELAPRVNEPPPPRLAETRI
                     NFALPGTKLPQLMRMYRVPGYVNAPRGTAEDMEVMTAILGNGATSRLYRTLVVDKKLA
                     VEAGAAYDGHNRGPGTFVVFAVPRDGIGFDTLETAMDQVIASMMRSPPEEGEFNRAKT
                     QLIADYTYQHDNQYLMAQDYGTALSIGLTIEDVEDWPNRIRAVTAAGVRKVAQTALVK
                     EEAVTGRMSPKAAQ"
     gene            complement(4400..>4657)
                     /locus_tag="VGM72_08985"
     CDS             complement(4400..>4657)
                     /locus_tag="VGM72_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265440.1"
                     /translation="LGANYSDGEKLLLTKTNALSVDPNVRHSVTSDVGQEDQGPAFSQ
                     KVLYSGAGTAPAMPAAAPAMSATAPAAAMAMPMPMTAPAKP"
BASE COUNT          833 a         1445 c         1442 g          937 t
ORIGIN      
        1 gaggcgcccc cgttcgcaac cgtcaccgcg atgcggcggg caccggcatc gagcgcattt
       61 tcggccagct ccttcaccgc ggcggccgga cgctcgacca cttcgccggc cgcgatacga
      121 ttgatcgtgc cttcggcgag acggcgaatg gcgcgcactg gcttggtctc aatccccatg
      181 gcggcccttg atcgactcgg cggaaattgt aacgcagttc cgagacaggg tgcagttttg
      241 gtccggcggc cggcggcatc cttccacagg acaatagagt ccgtcagggc cgcgggcaat
      301 caagagggac acgaaggcca caaaattagc acgaagagat ttttgtactc tttgtgactt
      361 cgtgccccgt tgtttcccac aagcaaatgg cgccaccgct ttcgcgggtg gcgccatttg
      421 gcccgctttt atcagcctaa tttattttcc gattggcggc aggccgctca catatttgaa
      481 gcgctgcacg cgctggccct gcacttcggc agagtagata ttgcccttgc tgtcgatggc
      541 gatgttgtga agattttcga actcgccggg accgcggccg acacggccga aactgccgag
      601 gatcttgccg tcgctgcgtt ggaagatgtg gatctggcca ttcggatcgt ctgaggtgaa
      661 ccagatgctc tgtcccttgt cgggccagaa cacggtggag ccggtcacgc caggcgccgt
      721 cgaatcgcgc agaagcgcga tctcgcggat gaaggtgcca tccttcttga agatctggat
      781 gcggttgttg gcgcggtcgg agaccacgac ttcgccgtca ttggtgatgc ggataccgtg
      841 gacgggattg ttgaattgct gcggcagcac cttgggatcg taggtgatct tctcgtcggt
      901 cggcggcttg ccataggcgc cccaggtccg cttgaacttg ccggtatcgg cgtcgaacac
      961 cacgacgcgg tgattgaaat aaccgtcggc gacataaagc tcattggtcg ccggatcgac
     1021 caccatgtcg gcggcgcggc ctacgcgcgt ctggtccagg ctgttggtct ggggcgcgga
     1081 tttgccgatc atcatctcga atttgccgtc gatggtgaac ttgacgatca tgccgtcttt
     1141 ctcgccattg ccgccgaacc agatattgtt tttgtaatcg aggaaaatgc cgtgctcatt
     1201 ggccggccag tcgaaaccct tggcggtttc cggcccgccc caggagcgga tcacatcgcc
     1261 cgcctggttg aattcgatga ccgagggcgc cgtcctgcaa caaatcgaat gatcgccgtc
     1321 ctgcatgcgg cgatcacgct tgttcaccgt gcgcgggcgc tgggtgatcc agacattgtc
     1381 gtccttgtcg acgaagatgc cgccgatctg accgaatatc caattgttcg gcagcggctt
     1441 gggccagctc gtgtcgatct ggaatttcgg ttcctccgca tatgcgaagc ccgtcaccgc
     1501 acatgccatg aacccggcaa gcgccaccat gcgaccaaat cgtgccacga aaaatccctc
     1561 cttgttatat gtcacggcgg gcgatctccg ccccgccgca agcttctaaa ctaacgccaa
     1621 tgccagggct cacgcaatct cgcgccgtgt ctatcgcgcg gcattgccgg taggcgcggg
     1681 cggtttggtt gcggcttccg gtgctgccgg cgtgcccgcg atgaccacca ccaaacgcgc
     1741 cggatcgaaa tatttcttcg ccatcgcgtt gacctgcgcg agtgtcacgg cctggatcat
     1801 ggcgttgcgc ttcacgacat aatcggccgc gaggcccgag cgctgatagg cgttcagcgt
     1861 gcgcgcgatc ttggcgttgg aatccagtcc caaagggaag gacccggtga gataggtctt
     1921 ggcatcgtcc aattccttgg cggcggcgcc atccttggca aagcgtgcca tctccgattt
     1981 cgtcacatcg agagcggtga ggattttggt cttgtcgctc tggaccgagc ccacgatgac
     2041 agaggcggaa cgaaaatcat taagctgcgt cgaaatccca taggtcagtc cgcgcttgtc
     2101 ccgtacctca tccatcaggc gtgctgagaa tcccccgccg ccgagaatat aattggtgac
     2161 gaaggtggga atgaaatcgg ggtccgcgcg catcggcccg gcaaagccga aaatcgccac
     2221 cggcgcggcc tcgttgcgca ccacgttgcg ggtgccgggc ctgccggcat cgaccggctt
     2281 cgagaccggc agaccggact tcacaggcag gggcgtgaag agcgcttgca ggtatttctt
     2341 cagctgcacc tcggtgacat caccggagac cgccattttt atgccgccac gcacgagatg
     2401 ctccgccgca aacgcccgaa tgtcgtcggc acgaatggca gcgatccccg cagccgtccc
     2461 cgccggcgga cgcccatagg gatgagcccc gaaataggcc gcataccagg ctttgaccgc
     2521 ggcccgcgat gggtcttcgt cttcctgttt caggctggca agcagcgcca cgcggatgcg
     2581 ctcgacggct tcgggttcga aacgcggatg cgcaaaggca agccccgcga ggcggaaagc
     2641 ctcatcggcg ttttctttca gcgtggtcag tgtgacgacg atgtaatcgc ggtcggcggt
     2701 ggcctgaaac cggatggcgc gggattcgag cgcctgcttg aatgcgtccg acgccagatc
     2761 gcccgcgccc tcgtccagca gcgaagccac catattggcg agccccggtt tgtcggccgg
     2821 atcgtaggcc gaccctgccg gaaacgagat gtttacagcg atcatcggca gagcacgctc
     2881 ttccgaaagc caggtctcca cccctgtcgg tcccgccacg gccttgaccg tgatggcgtt
     2941 cgcggccgcc ggaacgaaca ggaccaggaa tgcgaaaata agggaccgca gcgtcattgg
     3001 gccgccttcg gtgacatccg gccggtcacc gcttcctcct tgacgagggc ggtctgcgcc
     3061 actttgcgca cgccggccgc cgtcacggcc ctgatccggt tcggccaatc ctcgacatcc
     3121 tcgatggtga gtccgatgga gagcgcggtg ccgtaatcct gcgccatcag atattggttg
     3181 tcgtgctgat aggtgtagtc cgcgatcaac tgggtcttgg cgcggttgaa ttcgccttct
     3241 tccggggggc tgcgcatcat ggacgcgatc acctgatcca tcgcggtttc cagcgtgtcg
     3301 aagccgatgc cgtcgcgcgg caccgcaaag accacaaacg tccccggccc gcgattgtgg
     3361 ccgtcatagg cggcacccgc ttccaccgcg agcttcttgt ccacgacaag ggttctgtac
     3421 aaccggctgg tggcgccatt gccgaggatg gcggtcatca cttccatgtc ctcggccgtg
     3481 ccgcgcggtg cgttgacata accgggcacg cggtacatgc gcatcaattg cggcaatttc
     3541 gttccgggca aggcgaaatt gatccgcgtc tcggcaagcc gtggcggcgg cggttcgttc
     3601 acgcgcgggg caagttcgcg cgagggcacg gcgccatatt tgtcctcggc gattttgcga
     3661 acctcatccg gcgtcacatc gccgaccacc atcacgatgg cgttgttggg cgcgtaatga
     3721 tgctgataga aatcattcgc ctgtacacgg ccaatgctgc ggatctcttt ttcccagccg
     3781 atgacagggc ggccataggg atgggagaga tgaagcgcgg ccgccatctg ttcctgcgcc
     3841 agagcctgcg gctcggaatc cacgcgcagg tggcgctctt ccaggacgac atcgcgctcg
     3901 gtcgtcacat tcggttcgga aagatcgaga ttgaccatac ggtcggcctc cagccccatc
     3961 acgatcggca agcggtcctt ggcgatgcgt tggaaataga ccgtgaaatc atgcgaggtc
     4021 tgggcattat cctgtccgcc gttgcgggca atgatcttgg acagctcgcc attgggcacg
     4081 gtcttcgtgc ccttgaacat cagatgctcg aaaagatgcg cttccccgga aaggcccggc
     4141 ggatcgtcgg cgcctccgac gtgataccag accatgtggg tgacgaccgg cgcgcgatga
     4201 tccgggatca ccacgagctg cagcccgttt ttcagcatga attgcgtggc cttattcgcc
     4261 gccttgaccg gagcggtctc cggagcggag gcgtcggcat ggcgcacgaa ataccccgtg
     4321 acggcgagcg ctcctacgcc cgccccaaga agggcgattc ccgcaagcgc gtttctgacg
     4381 gcccgcatcc gccgatccgt cagggtttgg ctggggccgt catcggcatc ggcatggcca
     4441 ttgccgctgc cggagcggtt gccgacattg ccggtgcggc cgcaggcatt gccggtgcgg
     4501 ttcccgctcc ggaatagagc accttctggg agaaggcggg accctggtcc tcctggccca
     4561 catcggaagt gacgctgtgc cggacattgg gatcgacgct caacgcgttt gttttcgtca
     4621 gcaacagctt ctcgccgtcg ctgtaattgg cgcccag
//
LOCUS       DASYEG010000200        11228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4119841, whole genome shotgun sequence.
ACCESSION   DASYEG010000200 DASYEG010000000
VERSION     DASYEG010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 11228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11228
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4119841"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VGM72_08990"
     CDS             <1..663
                     /locus_tag="VGM72_08990"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265441.1"
                     /translation="RLDVELTDYGFQYSGIRKQNGRHWVRVYQYIMPVTQIRGRIAPV
                     RGEGAAPKVPLISGHYWVPIDDVTVSVFNFSYSADPAIALPREFALAAEADYGRGPGD
                     LLPDLRLKKSAANDYLIDRSLQKTQSFTGIGGINTQDVAVQEGMGPIADRSQEHLGST
                     DRAIVTMRRLLMEAARAIEAQGVPRGVDSASYRTVRAVDHFAGDESEIPALIARELRA
                     RF"
     gene            714..1232
                     /locus_tag="VGM72_08995"
     CDS             714..1232
                     /locus_tag="VGM72_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265442.1"
                     /translation="MWIEPETPDAWERWVRATGLHHFIAEHYWLWPMSETLHYLGLSL
                     LLGTVGLFDLRVLGMAKAIPPSALHKLIPLGIAGYCMNIVTGLAFFSGFPEQYAYNPS
                     FFWRGVFMAVAGMNVAVFYLSSAFAQVKAMPAGSDAPFRAKLIAGTSLGAWVAVLICG
                     RLLTFFRPPIFH"
     gene            1323..1955
                     /locus_tag="VGM72_09000"
     CDS             1323..1955
                     /locus_tag="VGM72_09000"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY4265443.1"
                     /translation="MKIQIALVAAIAATALAGTSMAASLSDVVQKGSAAEKIMEKNQI
                     NWATAKAIGDHCINEAAAKGMGVSVVIIDQFGVISYYGRGDGQGRTNTESALWKAKTV
                     LNTRAPSKAQMNAVRTGAVSESRVIWQGNFANGGGLPIVVDGQFLGAIGVGGMASTPP
                     AWSDEICGYNALTAVLGPQPKLLADLPNPYAISNNAAGGAGARPPGAANR"
     gene            2099..3040
                     /locus_tag="VGM72_09005"
     CDS             2099..3040
                     /locus_tag="VGM72_09005"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEY4265444.1"
                     /translation="MIRSFAATAGGPLLWIDLLQPTEDERRQITEQHKIHVPSRDELS
                     EIESSSRLFSREGLVCVSMSAIPPKDSTDLAPPPLGLVLTPAVLVTIRFSEIHGIERT
                     ASLFNTATPPATSVHAFVSILEAMVDYGADLLEHLAQQVTGISRKSFRQYAGAPRQVR
                     KTTRTLRAALVEIGSAGEHLSGARETLLGLQRIASFAPEAARGWFPDDVHERLQTVAR
                     DVASLTDFETHLSDKVQFLLDAVLGFINTEQNEIFKVLTIASVVGIPPTLIASMYGMN
                     FKNMPELSWAYGYEWGLGLILISTLLPILWFKSRGWW"
     gene            3152..4465
                     /gene="soxC"
                     /locus_tag="VGM72_09010"
     CDS             3152..4465
                     /gene="soxC"
                     /locus_tag="VGM72_09010"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR04555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HEY4265445.1"
                     /translation="MADTHRTAHPQVPHAAANGLLDRRALLGQGVVFAGAAAASVTGI
                     ASAAAEPLKVDPWSTTIGAPVTTYSLPSHFESKVIRTIGDNSRPGTQQARTPHQDLDG
                     IITPAALHFVVARAGAPDIDPGKHRLLIHGMVKQPLIFSLNDLMRYPMESHIRFIECG
                     GNSAPMWSKTPIQASVQALHGLLSCSEWTGIRLSTLLDEAGVDPKASWIIAEGADGVT
                     MNRSIPVAKAMDDAMIVFYQNGERIHPWNGYPMRLLVPGYEGNMNVKWLRRLKLTGAP
                     VMAINESRQYTLLLESGKAWRFYYPEEVKSFITRPSPTMTMPAPGYYEISGLAWSGHG
                     RIEKVEVSADGGKSWALAALQAPVLPKALTRFRMPWNWSGQPVVLQSRATDEGGNVQP
                     TRERLMAERGEPRNTPNVAAFPMNHVNCISSWGIDAKGAVSHVYV"
     gene            4452..4985
                     /locus_tag="VGM72_09015"
     CDS             4452..4985
                     /locus_tag="VGM72_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY4265446.1"
                     /translation="MSMFRTLAAACALALMSGAAWAADAPKFGQPITEQDAAAWDISI
                     APDGKGLPAGSGTPAQGAVIYAQKCAACHGEKGEGGTGPRLFGGIGTLKAPQQPVQTV
                     GSYWPYATMVFDFVRRAMPWDRPKSLTNDEVYASVAYILSLNGLIKDGDVLDKDSIMK
                     VQMPNRDGFIDLYPAKY"
     gene            5050..5643
                     /locus_tag="VGM72_09020"
     CDS             5050..5643
                     /locus_tag="VGM72_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265447.1"
                     /translation="MRFMGILSIPILCALTGPASAELRDSYTPVTIVALGTIAEPDDY
                     AVLSNGTPQPSFAQSMAGQDLHLGEELHSAIAAALLAKKLKVIPADGENAHYRLDVAI
                     PLHTVAYSDSVTDSELMPGFVVLVMVRDMRTGEIRLRDRIVYGRTDIESGHVIDGDAR
                     YRFASEDALLAHPKMAAEGLRAGIAAVADEIAGIMAK"
     gene            complement(6059..6349)
                     /locus_tag="VGM72_09025"
     CDS             complement(6059..6349)
                     /locus_tag="VGM72_09025"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY4265448.1"
                     /translation="MPSGFVYILASKRNGTLYTGITSDLLGRVVAHREGRGSEFVMKY
                     GVTRLVWFEEYPLYADAIQRETSLKRWKREWKLALIEKTNPDWDDLLEKWNA"
     gene            6570..6917
                     /locus_tag="VGM72_09030"
     CDS             6570..6917
                     /locus_tag="VGM72_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265449.1"
                     /translation="MGGKTNDVSNAEAVNAATAEAARIMTANLAAVSDMLQTSLKAAD
                     EVEDWDDQQAHLENALRLAEAAARLSEAFARVKGELRQTISVERSSESAERAPKASGI
                     APQFAPNKITQIG"
     gene            complement(7029..8414)
                     /locus_tag="VGM72_09035"
     CDS             complement(7029..8414)
                     /locus_tag="VGM72_09035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4265450.1"
                     /translation="MSWSQTALDSTRRTLLRAPLHPFRALKRLMPSGLFGRSLIIILA
                     PMLILQAVVSAVFFDRHYRVVTATMTRGVANDVGYMVMLENKLPLGPARDREREIASK
                     LFGYPAEFTPGERLTRTVSTPGTVLDRQLTYIFSSQMPEAVSFDTRRFTDYVDLRVQL
                     KDGVLQLLVPRERVTASNADIFILWMIGSSLVLIGIAALFLRNQVKPIERLAYAAESF
                     GKGRNVSEFKAYGAAEVRRAATAFMQMRERIERFVQQRTDMLAGISHDLKTPLTRLKL
                     QLALMPRDEDTEAMQGDIAEMERMLNEYLEFARGEGGGVAEPADLGVLAAEAVADVGR
                     AHDAASRITLFIGEPLTVAVKRNALKRCLVNLIDNALKYGGCVNVTLRRMGRRVELIV
                     DDDGPGIAEDLREEAFRPFHRLDEGRNLQAGGVGLGLAIARDIARNHGGDVRLEKSPL
                     GGLRAILRLPI"
     gene            complement(8386..9081)
                     /locus_tag="VGM72_09040"
     CDS             complement(8386..9081)
                     /locus_tag="VGM72_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4265451.1"
                     /translation="MNASDNPHLLVVDDDQRLRSLLQRYLSANGFRVTAAADAAEARS
                     LIKSIAFDGMILDVMMPGESGLDLASDLRAQSDLPILMLTALGDSEDRIAGLERGVDD
                     YLPKPFEPRELALRVNALIRRARSIRPPPHREVRMGACVFDAERGELTRNGKRVKLTS
                     SEVLMLKFFAGNAGRSFSRAELCTRLNIQLERSVDVQVTRLRRKIEEDPKLPLYLQTV
                     RGVGYVLVPDRVG"
     gene            9232..10152
                     /locus_tag="VGM72_09045"
     CDS             9232..10152
                     /locus_tag="VGM72_09045"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEY4265452.1"
                     /translation="MAGIIPFDDRDGSLWYDGRLVPWRDAKTHVLTHGLHYGSCVFEG
                     ERIYAGHIFRHDEHVKRLFESARILGMKIPYTEDEIARACYAAAAVQGITDGYVRPVV
                     FRGSEMMAVAAQQTKIHVAVAVWQWPSYFDPKEKLKGIRLDFAQWRRPAPDTAPTKAK
                     AAGLYMICTLSKHAAEAKGYADALMLDYRGLVAEATGANVFFVKDGQLHTPTPDCFLD
                     GITRRTVIALARTRHIPVHERAINPEELAGFEECFITGTAAEVTPVSEIGPYRFTPGI
                     ICETLMNDYTAEVNRPVPADAARERINASA"
     gene            complement(10136..10579)
                     /locus_tag="VGM72_09050"
     CDS             complement(10136..10579)
                     /locus_tag="VGM72_09050"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEY4265453.1"
                     /translation="MSEIRIQAQDFDPSVEIAALDDASGSVGAIATFIGLVRGDDGLI
                     AMTLDHYPGMCEREIAVHVAEARKRWPITGLRIVHRIGRLVPGERIVFVGVASSHRQA
                     AFQAAQFLMDYLKTRAPFWKREERQGGETWVEAKAADDESVKRWR"
     gene            complement(10576..10827)
                     /gene="moaD"
                     /locus_tag="VGM72_09055"
     CDS             complement(10576..10827)
                     /gene="moaD"
                     /locus_tag="VGM72_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEY4265454.1"
                     /translation="MKLLYFAWLRQKIGVSEEELSVPSSVATVAALIDCLKSRGAGYE
                     AAFRDLKALRVAVNQQHSRFDAAIGPNDEIAFFPPVTGG"
     gene            complement(10835..>11228)
                     /locus_tag="VGM72_09060"
     CDS             complement(10835..>11228)
                     /locus_tag="VGM72_09060"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY4265455.1"
                     /translation="AALLMLASDQMLQGWTFLVPALVILCREILVSGLREFLAEANVS
                     LPVTRIAKLKTVMQVVAIAALIASSASERMLPGVTAAALLGLWAAAGLTFYTGYAYLQ
                     AGLHHARRVSLSRPTANVGAKRPEQRTA"
BASE COUNT         2139 a         3510 c         3487 g         2092 t
ORIGIN      
        1 cgtcttgacg ttgaactcac cgattacggt ttccaatata gcggcatccg caaacagaac
       61 ggccgtcact gggtgcgggt ctatcaatac atcatgccgg tgacgcaaat ccgcggacgc
      121 atagcgccgg tgcgcggtga gggcgcagcc ccgaaggtcc ccctcatcag cggccattat
      181 tgggtgccga tcgacgatgt caccgtgtcg gtgttcaatt tctcctattc ggcggacccc
      241 gccatcgcgc tgccgcggga attcgcattg gcggcggaag ccgattatgg ccgcggcccc
      301 ggcgatctcc tgcccgatct gcgcctgaag aaaagtgccg cgaacgacta tctgatcgac
      361 cgcagcctac agaagacgca gagcttcacc ggcattggcg gcatcaacac acaggacgtg
      421 gcggtgcagg aaggcatggg gccgattgcc gatcgttcgc aagagcatct cggctccacc
      481 gaccgcgcca tcgtgacgat gcggcgcctg ctgatggaag ccgcgcgcgc catagaggcg
      541 caaggcgtgc cgcgcggcgt ggacagcgcc tcctatcgca cagtccgcgc ggtcgatcat
      601 tttgccggcg atgaaagcga gatccccgca ttgatcgccc gggagctgcg ggcgcggttt
      661 tagaatgcga gcgcgcgctc gccggagatg atttttgcct ttattgtcca tccatgtgga
      721 ttgaacccga gacgcccgat gcctgggagc gctgggttcg cgcgaccggt ctgcatcatt
      781 ttatcgccga acattattgg ctgtggccga tgtccgagac attgcattat ctcggtctgt
      841 cgctgcttct cggcacggtg ggtttgttcg acctgcgcgt gctgggcatg gccaaagcca
      901 taccgccctc ggcgctgcac aagctcatcc cgctggggat cgccggctat tgcatgaaca
      961 tcgtcaccgg tctcgcgttc ttctccggtt ttcccgagca atatgcctac aacccctcct
     1021 tcttctggag gggcgtcttc atggcggtcg ccgggatgaa tgtcgcggtc ttctatctgt
     1081 cgagcgcctt tgcgcaggtg aaggcgatgc cggcgggcag cgatgcgccg tttcgcgcca
     1141 agctgatcgc cggaacctcg ctcggcgcct gggtcgcggt cttgatctgc gggcgtctgc
     1201 ttactttttt ccgtccgccc atatttcatt gacgcggcgc caaggttgaa agcattcccc
     1261 caaaaaggca aaatggcttc agatgctgga cctggcatcg aaaacaccgg agggaaagca
     1321 ccatgaagat tcaaatcgcg ctggttgcgg ccatagccgc caccgcattg gcaggaacga
     1381 gcatggcggc gtcgctgagc gatgttgtcc agaaaggctc cgcggccgaa aagatcatgg
     1441 aaaagaacca gatcaactgg gcgaccgcca aggccattgg cgatcattgc atcaacgaag
     1501 ccgccgccaa gggcatgggc gtcagcgtcg tgatcatcga tcaattcggg gtcatttcct
     1561 actacggccg tggtgacggt caggggagga ccaacaccga atccgcattg tggaaggcaa
     1621 agacagttct caacacccgc gcccccagca aggcgcagat gaatgcggtt cgcaccggtg
     1681 cggtatccga gtcgcgcgtg atctggcagg gcaatttcgc caatggcggc ggtcttccga
     1741 tcgttgtcga tggccagttc cttggcgcca tcggcgtcgg cggcatggcg tcgacccctc
     1801 cggcatggag cgacgagatc tgcggctata acgccctgac ggcggtgctg ggtccgcagc
     1861 ccaaactgct tgctgaccta cctaacccct atgccatcag caacaatgcc gctggcggcg
     1921 cgggcgcccg cccaccgggt gcggcgaaca gataggccgt cctggtatcg ttcgccaagg
     1981 cctcgcgcat ccctgcgcgg ggcctttttc tttggcccgt tgcgcacggc ccgtgtaaca
     2041 gggtgcattg gacgatggcc gggaacggcc gccggttcca aagcgaatgg agacgtcctt
     2101 gatcagatcg tttgcggcta cggcgggtgg gcctctcctc tggatcgacc ttctgcagcc
     2161 gacggaagac gaaagacgcc agataacgga gcagcacaaa atccatgttc cgtcgcgcga
     2221 tgaactgagc gagatcgaat cctccagccg tctgttcagc cgtgagggtc tggtttgcgt
     2281 cagcatgtcc gcgattcccc cgaaagacag caccgatctt gcgccgcctc cactcggttt
     2341 ggtcctgacc cccgctgtgc tcgtcaccat ccgtttctcg gaaattcacg gcatcgagcg
     2401 gactgcatcg ctgttcaaca ccgccacacc tccggcgacg agcgtccatg cgttcgtttc
     2461 gattctcgaa gccatggtgg attacggcgc cgatcttctc gagcacctcg cccagcaggt
     2521 cacggggatt tcgcggaaat ccttccgcca atatgccggc gcgcccagac aggttcgtaa
     2581 gacgacccgg accctcaggg ccgccttggt cgaaatcggc tcggccggag agcacctctc
     2641 gggcgcgcgc gagacccttc tgggtcttca gcgcatcgcg tcgttcgcac cggaggcggc
     2701 gcgtggctgg ttcccggacg atgttcacga acggcttcag acggtcgcgc gagacgtggc
     2761 ctcgctcacc gatttcgaga cccatctgtc cgacaaggtt cagttcctgc tggatgcggt
     2821 tctgggcttc atcaacaccg aacagaacga gattttcaaa gtgctcacca tcgcatcggt
     2881 ggtcgggatt ccgccgacgc tgatcgccag catgtatggg atgaatttca agaacatgcc
     2941 ggaactgagc tgggcttacg gctatgaatg gggcttgggc ttgatcctta tcagcacact
     3001 tctgcccatt ctatggttca aatcgcgtgg ctggtggtaa ggggccgcgc cacggtcaat
     3061 aatccgccca cccgtgatat ccgcgcgagt acaatgacgg cgcgcgcgaa tgcgttagac
     3121 tgattgtcgt ctgcgaacca gggagggtct cgtggccgac acgcatcgca ccgctcatcc
     3181 gcaagttccg cacgccgccg caaacggatt gctcgaccgc cgcgcgctgc ttggccaggg
     3241 cgtcgtcttc gccggcgctg ccgcggcgag tgtcaccggc atcgcatcgg cggccgccga
     3301 gccgctgaag gtcgacccct ggagcacgac catcggtgcg ccggtcacaa cctattcgct
     3361 gccctcgcat ttcgaatcca aggtcatccg caccatcggc gacaattcgc gccccggaac
     3421 acagcaggcg cgtacgccgc atcaggatct ggacggcatc atcacgccgg ccgcgctgca
     3481 ctttgtcgtc gcgcgcgccg gtgcgcccga tatcgatccc ggcaagcacc ggcttctgat
     3541 ccacggcatg gtaaagcagc cgctgatctt ttcgctgaac gatctgatgc gctatccgat
     3601 ggaatcgcat atccgtttca tcgaatgcgg cggcaattcc gcgcccatgt ggtccaagac
     3661 gccgatccag gcgagtgtgc aggcgctcca cggcttgctg tcctgttccg aatggaccgg
     3721 catccgcctt tcgaccctgc tggacgaagc cggagtcgat cccaaagcga gttggatcat
     3781 cgccgaaggc gcggacggcg tgaccatgaa tcgttccatt ccggtcgcca aggccatgga
     3841 cgatgccatg atcgtcttct accagaatgg cgaacgcatc catccctgga acggctatcc
     3901 gatgcgtctt ctggtgcccg gttatgaagg caacatgaac gtcaaatggc tccggcggct
     3961 gaaactgacc ggcgctcccg tgatggccat caacgaatcg cgccagtaca ccttgctgct
     4021 ggagagcggc aaagcctggc gtttctatta tcccgaagag gtcaaatcct tcatcacgcg
     4081 gccctcgccc accatgacca tgcccgcacc cggctattac gagatcagcg gtctggcatg
     4141 gtccgggcac ggacgaatcg aaaaggtgga agtctccgcc gatggcggca agagttgggc
     4201 gctggccgcc cttcaggcgc cggtgctgcc caaggcgctg acccggttcc gcatgccgtg
     4261 gaattggagc ggccagccgg tggtgctgca gagccgggcc accgacgaag gcggcaatgt
     4321 ccagccgacg cgcgaacggc tcatggccga acgcggcgag ccgcgcaaca cgcccaatgt
     4381 cgccgccttc ccgatgaacc atgtgaattg catttcgagc tggggcatcg acgccaaggg
     4441 agcggtcagc catgtctatg tttagaaccc ttgccgcggc gtgcgcgctg gcgctgatgt
     4501 ccggtgcggc ctgggccgcc gatgcgccga aattcggcca acccatcacc gaacaggatg
     4561 ccgccgcctg ggatatctcc atcgcccccg acggcaaggg gctgcccgcc ggttccggta
     4621 cgcccgcgca aggggctgtc atctacgcgc agaaatgcgc cgcgtgccac ggcgaaaagg
     4681 gcgagggcgg caccggcccg cgcttgttcg gcggcatcgg caccctgaaa gcgccgcagc
     4741 agccggtgca gacggtcggt tcctattggc cctacgccac catggtgttc gatttcgtgc
     4801 gccgggcgat gccctgggac aggccgaaat cgctgaccaa cgacgaggtc tacgcgtcgg
     4861 tcgcctatat cctcagcctt aacggcctta tcaaggacgg cgacgtgctc gacaaggaca
     4921 gcatcatgaa ggtgcagatg cccaatcgcg acggcttcat cgatctctac ccggccaaat
     4981 attagggttt ttgccgcggc ggtcctgaaa ttcgcttgtg gtttgccaaa gcgggattac
     5041 gctcggccca tgcgtttcat gggcatcctc tcgatcccca ttttatgcgc cctgaccggg
     5101 ccggcatcgg ccgagctgcg cgattcctac actcccgtca ccatcgttgc gctgggcacg
     5161 attgctgagc ccgacgatta tgccgtcctt tccaacggca cgccgcagcc gagtttcgcg
     5221 cagagcatgg cggggcagga tctgcatctg ggcgaggaac tgcattccgc gattgccgcg
     5281 gcgctcctcg ccaagaagct gaaggtcatt cccgccgatg gggaaaacgc ccattaccgg
     5341 ctggacgtcg ccattcccct gcacacggtc gcctatagcg actcggtgac ggattccgaa
     5401 ctgatgccgg gcttcgtggt gctggtgatg gtgcgcgaca tgcgcaccgg cgaaatccgg
     5461 ttgcgcgacc gcatcgtcta cgggcggaca gacatcgaaa gcggccatgt gatcgacggc
     5521 gatgcgcgtt accgcttcgc gtccgaagat gcgctccttg cccatcccaa aatggcggcc
     5581 gagggattgc gcgccggcat cgcggcggtg gcggacgaaa tcgccggcat catggcgaag
     5641 tagttttccc gcgtgaacac aattggcgtt tggaagaacc ggctacgccg cacgggtcgc
     5701 aatcggtgac ggagcgcttg cagagaacga tctcgttgat gcgacgtggc ggttcgcgga
     5761 tggcgcggca agcgcgtttg gctcaaattc atgagcctca ggaatttctt cagcaggatt
     5821 tctccctgtg taggattgcg caggttttct cgttatcgtg ttaccatctg catattctca
     5881 ccggcttact cctacaccaa cccttcacca tggccgggct tgacccggcc acccagtcag
     5941 ctcgcgtccg tgggctggtt gaatcgtgag tgcgctcgca taactagaaa agaatctttc
     6001 accgcaccga cgtgcggcgg ctgggtggcc ggctcatagg ccggccatgg tgaaaaagct
     6061 aggcattcca tttttcgagc aggtcgtccc aatcgggatt cgtcttttcg atcaatgcca
     6121 atttccattc gcgcttccag cgtttcaaac tcgtctcacg ctggatcgca tccgcatata
     6181 gcggatactc ctcgaaccat acgagacgag tgacgccgta tttcatgacg aactcggaac
     6241 cgcgtccctc gcgatgcgcg acgacacgac caagcaaatc cgaggtaatc ccggtgtaca
     6301 gcgtgccatt gcgcttgctg gccaggatgt aaacaaagcc gctcggcatt gtcgggtttt
     6361 agctcactgt ggctgtcgaa gccatttgcc gcacggacgt gcaatggctg ggtggccggc
     6421 tcataggccg gccatggtga atgggacgtg gcgcgcatta ttcaagggcg gcggggcacc
     6481 aaggcgcgaa aaaatttcgt aagctaaaat tatccacgct ccctgggacg gcttcataat
     6541 tctctccgca acgagagaaa gagacccgca tgggaggcaa aaccaacgat gtttccaacg
     6601 cggaagccgt gaatgcggcg accgccgaag ccgcgcgaat catgaccgcc aacctggcgg
     6661 cggtcagcga catgctgcaa accagtctca aggcggcgga cgaggtggaa gactgggacg
     6721 accagcaggc tcatctcgag aatgccttga ggctggccga ggcggcggca cggctgagtg
     6781 aggctttcgc gcgggtgaag ggcgaactgc gccaaacgat cagcgtcgag cgcagctcgg
     6841 aaagcgctga acgggcaccc aaagcgagtg gaattgcacc gcaattcgca ccaaataaaa
     6901 ttacgcagat cgggtaaaaa tccagtggtt tcgcagaaaa aatctgcgcg ccaaccctca
     6961 atcagggggg agggggatca gccccgtctt ccccaagggc cccccaaggg gagggcggaa
     7021 atgacggctc aaatcggcag gcggagaatc gcgcgcaaac cgccgagcgg gcttttctcc
     7081 agccgcacgt cgccgccatg attgcgcgcg atgtctcggg cgatggcgag gccaaggccc
     7141 acgccgccgg cttgcagatt gcggccctcg tccagccggt ggaatgggcg gaaggcttcc
     7201 tcgcgcaagt cctcggcaat gcctggaccg tcatcgtcca cgataagctc cacgcggcgg
     7261 cccatccggc gcagcgtcac attcacgcat ccgccatatt tcagcgcgtt gtcgataagg
     7321 ttgacgagac agcgcttgag cgcgttgcgc ttgaccgcga ccgtcagcgg ctcgccgatg
     7381 aagagcgtga tgcggcttgc cgcgtcgtgg gcgcggccga catcggccac ggcttcggcg
     7441 gcgagcacgc cgagatcggc gggttcggcg acgccgccgc cttcaccgcg cgcgaactcc
     7501 agatattcgt tcagcatgcg ctccatttcg gcgatgtcgc cctgcatcgc ctcggtgtct
     7561 tcatcgcggg gcatcagagc aagttgcagt ttcagccggg tgagcggtgt tttcagatcg
     7621 tggctgatgc cggccagcat gtcggtgcgc tgctgaacga aacgctcgat gcgctcgcgc
     7681 atttgcatga aggccgtggc ggcgcgtcgc acttcggcgg cgccataggc cttgaactcc
     7741 gacacattgc gccccttgcc gaaactttcc gccgcatagg cgagccgctc gatgggcttg
     7801 acctgattgc gcagaaacag tgcggcgatg ccgatcagga ccagcgagga gccgatcatc
     7861 cagaggatga agatgtcggc gttcgatgcg gtgacacgtt cgcgcggcac cagaagctgc
     7921 agcacgccgt ccttcaattg cacgcgcaga tcgacgtaat cggtgaagcg gcgggtgtcg
     7981 aagctgaccg cttcgggcat ttggctggag aagatgtagg tgagctggcg gtcgagcacg
     8041 gtgccgggcg tcgatacggt gcgggtgagc ctctcacccg gcgtgaactc cgccggatat
     8101 ccgaacagct tggacgcgat ctcgcgttcg cggtcgcgcg ccgggccgag cggcagtttg
     8161 ttctccagca tcaccatgta gcccacatca ttggccacgc cgcgcgtcat cgtggcggtg
     8221 acgacgcggt aatggcggtc gaagaagacc gcggagacga ccgcctgcag gatcagcatc
     8281 ggcgccagga tgatgatgag ggagcgtccg aatagcccgc tcggcatcag ccgcttcagc
     8341 gcgcggaatg ggtgcagcgg cgcacgcagc agcgtgcgcc gcgtgctatc caacgcggtc
     8401 tgggaccagg acatagccga caccacgaac ggtttgcaga tagagcggaa gcttggggtc
     8461 ctcttcgatc ttgcggcgca ggcgcgtcac ctgcacatcc accgagcgct ccaactggat
     8521 attcagccgc gtgcagagct cggcacggct gaaggagcgt cccgcattgc cggcgaaaaa
     8581 tttcagcatc agcacttcgg aactcgtcag cttgacgcgt ttgccgttgc gggtcagttc
     8641 gccgcgttcc gcatcgaata cgcaggcgcc catgcgcacc tcgcgatgcg gcggcgggcg
     8701 tatgctgcgc gcgcgccgga tcagggcgtt gacacgcaac gcgagttcgc gcggttcgaa
     8761 gggcttgggg aggtagtcat caacgccacg ctcaagcccg gcgatgcggt cctcggaatc
     8821 gcccagcgcc gtcagcatca aaatcggcag gtcggattgc gcgcgcaggt ccgatgccag
     8881 gtccaggccg ctctcgccgg gcatcatcac gtccaggatc attccgtcga acgcgatgct
     8941 cttgatcagg cttcgcgctt cggcggcatc ggcggcggcc gtcacgcgaa atccgttcgc
     9001 cgacagataa cgctgcagca ggctgcgcag gcgttggtcg tcatcgacga caagaaggtg
     9061 cgggttgtcc gaagcgttca tcggcgcatg ataggaagga actgtaccgg gccaaagcgt
     9121 gtgggccatt atgttgacat cattaggccg cccccctagg cgtagcaagc acgataccct
     9181 aacttccggg gcgcgggggc tgcgcgtgga tgaagcattc ccgaggacat catggctggc
     9241 atcattcctt tcgacgatcg cgacggttct ctctggtatg acggcaggct ggtgccttgg
     9301 cgcgatgcca agacccatgt gctgacccat gggctgcatt atgggtcctg tgtcttcgaa
     9361 ggcgagcgca tctatgcggg gcacatcttc cgtcacgacg agcatgtgaa gcggctgttc
     9421 gaatcggcgc gcatcctcgg catgaaaatt ccttatacgg aggatgagat cgcgcgcgcc
     9481 tgctatgcgg ccgccgcggt ccagggcatc acggacggtt atgtgcgccc agtggtcttt
     9541 cgcggcagcg agatgatggc cgtggctgcc cagcagacga agatccatgt cgcggtcgcg
     9601 gtgtggcagt ggccttccta tttcgatccc aaggaaaagc tcaaaggcat ccggctcgat
     9661 tttgcccaat ggcggcggcc cgcgcccgat accgcgccga cgaaagccaa ggcggcgggt
     9721 ctctacatga tctgcacgct gtcgaaacac gccgccgaag ccaaaggcta tgccgatgcg
     9781 ctgatgctcg attatcgcgg gctggtggcg gaggccacgg gtgccaacgt attcttcgtc
     9841 aaggacgggc agttgcacac gcccacaccc gattgcttcc tcgatggcat tacgcgccgc
     9901 acggtgatcg cgcttgcgcg gacgcgccac attccggtgc acgaacgcgc gataaaccct
     9961 gaagaactcg ccggtttcga agaatgcttc atcaccggga ccgcggcgga agtgacgccg
    10021 gtctccgaga tcggtccgta tcgattcacg ccgggcatca tctgcgaaac gctgatgaac
    10081 gactacaccg ctgaggtcaa tcggccggtc ccggccgatg cggcgcgcga gaggatcaac
    10141 gccagcgctt gacgctttcg tcgtctgcgg ctttcgcctc gacccaggtc tcgccgccct
    10201 gccgctcttc gcgtttccag aacggggcgc gggtcttgag gtaatccatc aggaactggg
    10261 ctgcctggaa tgccgcctgc cggtgtgagg aggcaacccc gacgaacacg atgcgctcgc
    10321 ccggaaccaa gcggccgatg cgatgcacga tacgcaaacc ggtgatgggc cagcgttttc
    10381 tggcttcggc gacatggacc gcgatctcgc gttcgcacat gcccggataa tggtcgaggg
    10441 tcatggcaat gagtccgtca tcgccgcgca ccagtccgat gaaggtcgcg atcgcgccga
    10501 ccgaacccga agcgtcgtcg agcgcggcaa tctccacact gggatcgaaa tcctgcgcct
    10561 ggatgcgaat ttcgctcatc cgccggtgac cggaggaaag aacgcgattt cgtcattcgg
    10621 gccgatggcc gcatcgaagc gcgaatgctg ctggttcacc gcgacgcgca acgccttcaa
    10681 atcgcgaaac gccgcctcat accccgcgcc gcgcgacttc agacaatcga tcaatgccgc
    10741 aacagtcgcg acggaagaag gaacactcag ttcttcttcc gagacgccga tcttctggcg
    10801 caaccaggca aaatagagga gcttcacgtg cccctcacgc ggttctctgt tcgggacgct
    10861 tggcgccgac attcgcggtg gggcgcgaca ggctcacgcg ccgggcatga tgcaaccccg
    10921 cctgcaaata ggcatagccg gtatagaatg tgagcccggc ggcggcccag aggcccagga
    10981 gcgcggcggc ggtgacgccg ggcagcatcc gttcgctggc cgaacttgca atgagtgccg
    11041 cgattgccac cacctgcatc accgtcttca atttggcgat gcgtgtgacc ggcaggctga
    11101 cattggcctc ggccaggaac tcgcgcagac ccgaaaccag gatttcccgg cacaagatca
    11161 ccagtgccgg taccaaaaac gtccatccct ggagcatctg atcggacgcc agcatcaaga
    11221 gtgccgcc
//
LOCUS       DASYEG010000201         8589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4120026, whole genome shotgun sequence.
ACCESSION   DASYEG010000201 DASYEG010000000
VERSION     DASYEG010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 8589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8589
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4120026"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VGM72_09065"
     CDS             <1..195
                     /locus_tag="VGM72_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265456.1"
                     /translation="GEALKDIWAVDLNKGGNAIPYHMEDPALRQEYDETLARSGLASR
                     MEPRFYEEAGAAAADSIGGF"
     gene            complement(241..363)
                     /locus_tag="VGM72_09070"
     CDS             complement(241..363)
                     /locus_tag="VGM72_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265457.1"
                     /translation="MGLATVRPLPIHGNPSSQADFERTLGPVAAAANPAGGEGG"
     gene            405..875
                     /locus_tag="VGM72_09075"
     CDS             405..875
                     /locus_tag="VGM72_09075"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY4265458.1"
                     /translation="MTDRSASMAELASSNQRTGILAGVSDMQLAYSFFRFSMGVVIFF
                     HGAMRMITGVDAWVAKESQIFVGDPLLPMWFVVAFLYVLPAIEVVLGTLTILGFYTRW
                     ALMIGAVLMLILVFGNLTRQDWGTVGNELHYVLYYALMIAGLKYNRFALDTRKG"
     gene            complement(878..1576)
                     /locus_tag="VGM72_09080"
     CDS             complement(878..1576)
                     /locus_tag="VGM72_09080"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEY4265459.1"
                     /translation="MPALAIRTSIAAAILALLPLPGVAAAEGTRSGSPEQQRIEDAYT
                     VRLQAAQEKAQDQRIAAQAKALLSDSASPVLGNPKGDVTIVEFFDYTCPYCKAVEPRL
                     QKLLKDDRNVKIVVKEFPILTPESLIAAKVALASQKQGKYLQYHQALMNFKGRLANDV
                     IFDTAKSVGLDVDRVRKDMEAPEITDEIIANFNLARSLRIFQTPAFISGGHMLDSTSA
                     NIDFTRVAASVRGK"
     gene            complement(1664..3070)
                     /locus_tag="VGM72_09085"
     CDS             complement(1664..3070)
                     /locus_tag="VGM72_09085"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4265460.1"
                     /translation="MALAQARPGEAGHPAGFIAARLDRLPATRTIWTRVVLLSLGGFF
                     EFYDLFFTGYVAPGLIRSGILTPTTPGLFGTNGIAGFVAALFAGLFLGTALFGFVADR
                     FGRRTIFTMSMLWYTAATVVMAFQKDAFGLNLWRFIAGIGIGVELVTVDAYIAELVPK
                     AARGRAFACNQVVQFMAVPVVAFLAWRLVPVAPLGLDGWRWVVLIGSAGALLVLWIRR
                     NVPESPRWLAEHGYRDEAERVLTQLEARVAAESGAALTSPEGAEAEVRPGRLAEIWRR
                     PFLTRTLMLIVFNIFQTVGFYGFSNWVPTLLIAQGIAVTTSLQYTFIIATASPVGPLL
                     GVLFADRFERKWLVVAAALCIAVFGLIFAQMTTAASLVFFGVLVTLSNNILSFSYHAY
                     QAELYPTRIRALAVGFVYSFSRLSVVFSAFVIAFVLERFGVDGVFTLIAASMAIVMLA
                     IGLFGPRTSNRALEEIST"
     gene            complement(3074..3925)
                     /locus_tag="VGM72_09090"
     CDS             complement(3074..3925)
                     /locus_tag="VGM72_09090"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY4265461.1"
                     /translation="MKLVSFRVGAAAHYGVAVGTGIVDLTPRLGVRYADIKAVLAGGA
                     LGEASRAANGAPSDYRLDEIQFDPVIPNPGKILCVGLNYEEHRMETGRPKSENPSIFT
                     RFIDTQIGHLQPIVKPKLSDKVDYECELAVVIGKGGRYIPESAAYDHVAGYSCYNDVS
                     IRDFQNHTSQWTPGKNFPASGPFGPWLVTRDEMGELPGKKIQTRVSGERMQHADFDQM
                     IFPVPRIIEYISRFTTLAPGDVIISGTPGGVGFRRDPPRWMKAGETVEVEIDGIGILK
                     NTILAED"
     gene            4109..5476
                     /locus_tag="VGM72_09095"
     CDS             4109..5476
                     /locus_tag="VGM72_09095"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4265462.1"
                     /translation="MMAVERNLDVGELIEGQRLARPQVNIALLLCTLMVLEGYDMQAL
                     SFTTPAILSQWHVRPADFGFVATAHLVGYFVGAMVLSFFGDRIGRKNIIVTGAMIFGI
                     FTFAAGFAATPQELYLMRFGAGFGLGGAIPSGIALAAEYMPQRFRATMIGLMYVGYNF
                     GAGAGGFVASWTIKDYGWSSVFFIGGVAAVPMVAGLYFALPESVRFLIVNGAAPARIA
                     AIVRRLRPHTDLSGVARFVINEESGRNSPLSLLTEGRALVTVLLWISFITALAGHYFI
                     TLWMPKVLLDDGYALADANRAMGMFQFGGAIGSFLIAFLLNRMDIRIVAFTFLLTAPI
                     VAALSLHTGYGVLIPHMMIAGVGVLGGQIGLNALSGTIYPTYMRAMGAGWGLGIGRIG
                     SLAGPILGGYLIGMGIPRPTLLLLDSIPFLLCAVALYAMYAAKRARDGREGELPFAKA
                     GGFAH"
     gene            5555..6544
                     /locus_tag="VGM72_09100"
     CDS             5555..6544
                     /locus_tag="VGM72_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265463.1"
                     /translation="MNKLPLTAAMPEYEHVRDLALGRVQPEGIDLTLLTFPVEEIFYR
                     FIVYKEWDVSEISMAKYVSLIASGDQNFWALPVFPSRVFRHSSLYVRRDGPIKQIGDL
                     KGRRVGIPEWAQTAAVYSRGFLAHQYGLDLASINWVQAGVNQPGRVEKVELQLPKGVA
                     IERPAGKSLNGMLLAGEVDAILVARPPDAFLAGDPDIVRFFGDFLEVEQRYYKETGIF
                     PIMHTVAVRRDVLDKNPWVARNLFYAFDEARKRAIARALDNTVSLYPIPWGFEYARRG
                     LDMMGDDYFPYGMEKNRKTLEAFLGYAHEQGVCKRLLRIEELFPKQFLSVHKV"
     gene            6586..7659
                     /gene="gtdA"
                     /locus_tag="VGM72_09105"
     CDS             6586..7659
                     /gene="gtdA"
                     /locus_tag="VGM72_09105"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HEY4265464.1"
                     /translation="MTVYAMDSHAQLRKNADEFARYYGRLAKVNMAPLWEVMRNLVTP
                     EPVSPVQPAQWRYADVRPLLFESAAMITAEQAERRVLVLENPGLPKQSAITHSLYAGI
                     QLVMPGEIAPQHRHTQAALRFILEGRGATTTVNGERFEMFPGDMILTPSWQWHDHQNE
                     TEVPIVWLDGLDIPLVGLLDSSFAQTSNRKEQEVTREKETSQTMYGANLLPIDDAPVS
                     RNSPLMRYPYDTARATLERMKISSPLDPCHGIRMRYAHPRTGDWAMPTIGPAIQLVPK
                     GLATTPYRATDGTIFSVAEGQGTAKIGNAEFALSPRDVFVCPPWAWRSFKADDDLVLF
                     NFSDRPVQEKLGLWREERTPSVA"
     gene            7660..>8589
                     /locus_tag="VGM72_09110"
     CDS             7660..>8589
                     /locus_tag="VGM72_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY4265465.1"
                     /translation="MDSLKTRADPILIAGGGIGGLAAALALARRGFPSRVLEQAPEFK
                     EVGAGIQLGPNSWRCLRALGVEAAVKRLAVFPEALVMMDGVTAEPVVSIPLGGFETRF
                     KAPYALIHRADLHLCLLEACKASSHIALETSCLVTDFTDGGANVRVATGDRNFRDTPA
                     LIAADGLWSAARSKLIGDGSPRVSGHISYRSVLKPADVPENLRRNDMVLWAGPKCHMV
                     HYPLRGGELFNLVATFHSDRYEEGWNSFGDPAELHARFSGTCDIVQTMLGRIEEWRMW
                     VLCDREPIADWSKGRVTLLGDAAHPMLQYLAQGA"
BASE COUNT         1597 a         2620 c         2691 g         1681 t
ORIGIN      
        1 ggcgaagcgc tgaaggacat ctgggccgtc gatctgaaca agggcggcaa cgcgatcccc
       61 tatcacatgg aagaccccgc gctgcgtcag gaatatgacg agaccctggc gcgttcagga
      121 ctcgcctcgc gcatggagcc gcgcttctac gaagaagcgg gcgccgccgc ggcggactca
      181 atcggcggat tctgaacgca atcccaccct cccttgcgga agggtgggat acgctagaga
      241 tcagccgcct tcgccgccgg cggggttggc ggctgccgcc accggaccca gcgtgcgttc
      301 gaaatcggcc tgcgagctgg gattgccgtg gatcggcaga ggccgtaccg ttgccaaacc
      361 cacgcacagg gtgtgaaact cctgcaaaat ctggggagaa tttcatgacg gacagaagcg
      421 catccatggc ggagctcgcc agcagcaacc aacgcaccgg cattctggcc ggtgtcagcg
      481 acatgcagct tgcctattcg ttcttccgct tttccatggg cgtggtgatc ttcttccatg
      541 gtgccatgcg catgatcacg ggagtcgatg cctgggttgc caaggagtcg caaatcttcg
      601 tcggcgaccc gctgcttccg atgtggttcg tcgtggcgtt cctctacgtg ctgcccgcca
      661 tcgaagtggt gctgggcacg ttgaccatcc tgggatttta tacgcgctgg gcactgatga
      721 tcggcgccgt gctgatgctt attctggtgt tcggcaatct cacgcgccag gattggggca
      781 ccgtcggcaa cgagctgcat tatgtcctgt actacgcgct catgatcgcg ggcttgaaat
      841 acaatcgctt cgcgctcgat acgcgcaagg gatagcgtca cttcccacgg acggaggcgg
      901 ccaccctggt aaaatcgata ttggccgagg tgctgtcgag catatggccg ccggagatga
      961 aggccggcgt ctggaagatc cgcagactgc gggcgagatt gaaattggcg atgatctcgt
     1021 cggtgatttc cggcgcctcc atgtccttgc gcacgcggtc tacatccaat cccacgctct
     1081 tggcggtgtc gaagatcacg tcattggcca gccggccttt gaagttcatc agagcctgat
     1141 ggtattgcag gtacttgcct tgtttctggg acgcgagagc gactttcgcg gcgatcagag
     1201 attccggtgt caggattgga aactccttga cgacgatttt cacattcctg tcgtctttca
     1261 gaagcttctg cagccggggt tcgacggcct tgcagtaggg acatgtgtag tcgaaaaatt
     1321 cgacaatggt gacatccccc ttgggattgc cgagaacggg agaggcggaa tcgcttagga
     1381 gagcctttgc ctgggcggcg atgcgctggt cctgggcctt ttcctgcgcc gcctgtagcc
     1441 tgactgtata ggcatcttca atgcgttgct gttcggggct gccggaacgc gtcccttccg
     1501 ccgccgcaac gccgggaagc ggcagcaagg cgaggatcgc cgcggcaatg gacgtgcgga
     1561 tagccagtgc gggcatgagc ggttttccag aattcacagc ggctcgaaac taacagaaac
     1621 tttcccgtcc ggcaaaaacg caaatcggcg ccactctcac gcttcaggtg gaaatttctt
     1681 ccagcgcgcg attgctggtc ctgggtccga agagaccgat cgcaagcatg acgatggcca
     1741 tgcttgcggc gatgagcgtg aacacgccat cgacgccgaa acgctccagc acgaatgcga
     1801 tgacgaacgc gctgaagacc accgacagcc ggctgaagga atagacaaag ccgacggcga
     1861 gcgcgcggat gcgcgtggga taaagctcag cctgataggc atggtagctg aacgacagga
     1921 tgttgttcga gagcgtgacc agtacgccga agaaaacgag cgacgccgcc gtggtcatct
     1981 gcgcgaagat gaggccgaag acggcgatgc aaagcgcggc cgccaccacc agccatttgc
     2041 gctcgaaccg gtcggcgaaa aggacaccga gcagcggtcc gaccggcgag gccgtggcga
     2101 tgatgaacgt gtattgcagg ctggtggtga cggcgatgcc ttgcgcgatg aggagcgtcg
     2161 gcacccaatt ggaaaagccg tagaatccga ccgtctggaa aatgttgaac acgatcaaca
     2221 tcagggtccg ggtgagaaaa ggcctgcgcc agatctcggc caagcggccg ggccggacct
     2281 cggcttcggc cccttccggc gatgtcagcg ccgcgccgga ttccgcggcg actcgcgctt
     2341 ccaactgagt cagaacgcgc tcggcttcgt cccgatatcc gtgctcggcg agccagcgcg
     2401 gactttcggg gacgttgcgg cgaatccaca gcaccaacag tgcgccggcc gagccgatca
     2461 gaaccaccca gcgccagccg tcgagtccca aaggcgcgac cggcaccaat cgccaggcga
     2521 ggaatgcgac caccggcacg gccatgaact gcaccacctg attgcaggca aaggcgcgtc
     2581 cgcgcgccgc cttcggtacg agttcggcga tataggcgtc caccgtgacc agttcgacgc
     2641 cgatgccgat gccggcaata aagcgccaga ggttgagacc gaaggcgtcc ttttggaatg
     2701 ccatcacgac ggttgccgcc gtataccaga gcatggacat ggtgaagatc gtgcgccgtc
     2761 cgaagcggtc ggcaacaaag ccgaacaacg ccgtgccgag gaacagcccc gcgaacaacg
     2821 ccgcgacaaa gccggcgatc ccattggtgc cgaacagccc cggcgtcgtg ggcgtgagga
     2881 tgccgcttcg gatcagaccg ggcgcgacat agccggtgaa gaagagatcg tagaattcga
     2941 aaaatccgcc cagcgacagc agcacgacac gtgtccagat cgtgcgcgtc gcgggcaagc
     3001 ggtcgaggcg cgccgcgatg aacccggcgg gatggccggc ctcgccagga cgcgcctgcg
     3061 ccaacgccat gtgtcagtct tcggcgagga tggtattctt cagaataccg atgccgtcga
     3121 tctctacttc cacggtctcg ccggccttca tccagcgtgg cggatcgcgg cggaatccga
     3181 cgccgcccgg tgtgcccgat atgatcacat cgcccggggc cagcgtcgtg aagcgcgaga
     3241 tatattcgat gatgcgcgga accgggaaaa tcatctgatc gaaatcggcg tgctgcatgc
     3301 gttcgcccga aacgcgtgtc tggattttct tgcccggcag ctcgcccatt tcatcgcgcg
     3361 tcaccagcca cggcccgaac ggaccggagg ccggaaaatt ctttcccggc gtccattgcg
     3421 aggtgtggtt ctgaaagtcg cggatcgaga cgtcgttgta gcaggaataa cccgcgacat
     3481 gatcgtaggc cgcactttcg ggaatgtagc ggccgccttt tccgatgacc acggcaagct
     3541 cgcattcgta atccaccttg tccgacagct tgggtttgac gatgggttga agatggccga
     3601 tctgcgtgtc gatgaaacgg gtgaagatgc tgggattttc cgatttggga cggccggttt
     3661 ccatccggtg ttcttcatag ttcagtccga cgcacaggat ctttccgggg ttggggatca
     3721 ccggatcgaa ttggatctcg tcgagcctgt aatcggacgg ggcgccattg gccgcgcgcg
     3781 acgcttcgcc aagggcgccg cctgcgagca ccgccttgat gtcggcatag cgaacgccca
     3841 accggggcgt cagatcgaca atgccggtgc ccaccgcgac cccataatgg gccgcggccc
     3901 ccacgcgaaa cgaaaccagc ttcattgatg ctctcctgat tgtgacacag ttcttgcccg
     3961 gtgctgctca tagccgaatt tgcccggagc ttcacgcgca aaaaagcggc cgggatgggg
     4021 gccatgctcg gggccagttg attcacccaa aaactctgtc aatctgcgga cgggacgccg
     4081 ggacagagat tttccggccc gggaggagat gatggcggtc gaacgcaatc tcgacgtcgg
     4141 cgagctgatc gagggccagc ggcttgcccg tccgcaagtc aatatcgcgc ttctgctgtg
     4201 caccctcatg gtgctcgaag gctatgacat gcaggcgctg tccttcacga cgccggccat
     4261 tctgtcgcaa tggcatgtgc gccccgcgga tttcggtttc gtcgccaccg cgcatctcgt
     4321 gggctatttc gtcggcgcca tggtcctgag ctttttcggc gaccggatcg gacgcaagaa
     4381 catcatcgtc accggcgcga tgatctttgg aattttcacc tttgcggctg gcttcgcagc
     4441 cacgcctcag gaactttatc tgatgcgctt tggcgcaggc ttcggcctag ggggcgcaat
     4501 tccttccggc atcgcgcttg ccgccgaata catgccccag cgtttccgcg cgaccatgat
     4561 cgggctgatg tatgtcggct ataatttcgg cgccggcgcg gggggattcg tcgcgtcctg
     4621 gacgatcaag gattacggct ggtcgtcggt gttcttcatc gggggcgtcg ctgccgttcc
     4681 gatggtcgcc ggattatact tcgcgctgcc tgaatcggtc cggttcctga tcgtgaacgg
     4741 cgccgcgccg gcgcgcatcg ccgccatcgt gcgccggttg cgtccgcaca cggatttgtc
     4801 cggtgtcgcg cgtttcgtca tcaatgagga aagcggccgc aattcgccgc tcagccttct
     4861 caccgaaggc cgcgcgcttg tgaccgtgct gttgtggata tccttcatta ccgcgctggc
     4921 gggacattat ttcatcacgc tctggatgcc gaaggttctt ctggacgacg gctatgcgct
     4981 cgccgatgcc aacagggcga tggggatgtt ccagttcggc ggcgcgatcg gaagcttcct
     5041 catcgccttc ctgctgaaca ggatggatat caggatcgtc gcgttcacct ttctgttgac
     5101 ggccccgatt gtcgcggctc tcagcctgca caccggctat ggcgtgctga tcccgcatat
     5161 gatgatcgca ggggtcggtg tgctcggcgg ccagatcggg ttgaacgcgc tgtccggcac
     5221 catctatcca acctatatgc gtgccatggg tgcgggatgg ggcctcggca tcgggcgcat
     5281 cggttcgctt gcggggccca tcctgggcgg ctatctgatc ggcatgggaa tcccgagacc
     5341 gacgctgctg ttgctggatt cgatcccgtt cctgctctgc gcggtcgcgc tctatgccat
     5401 gtatgccgcc aagcgcgccc gtgatgggcg cgaaggcgaa cttcctttcg ccaaagccgg
     5461 aggcttcgcc cactgagact ttggcggccc gcgggggacg cgggacgcgc tccacggaaa
     5521 atcccggaat ttgaacccat catcggagtc agcaatgaac aagctaccgc tcacagcggc
     5581 aatgccggaa tacgaacatg tgcgcgacct tgcgcttggg cgcgtgcagc ccgagggcat
     5641 cgacctgacg ctgctcactt tcccggtcga agaaatattc taccgcttca tcgtctacaa
     5701 ggaatgggac gtttccgaga tctcgatggc gaaatatgtt tcgctcatcg cttccggcga
     5761 ccagaatttc tgggcgcttc cggtatttcc ttcacgcgtg ttccggcatt catcgctcta
     5821 tgtgcggcgc gatggtccca tcaaacagat cggcgatctg aaaggccggc gggtcggcat
     5881 tccggaatgg gcccagaccg cggccgtgta ttcgcgcggg tttctggccc atcaatatgg
     5941 gctcgatctc gcctccatca actgggttca ggccggcgtc aatcagcccg gacgggtgga
     6001 gaaagtcgaa ctgcaactgc ccaagggtgt cgccatcgag cgccccgccg gcaaatcgct
     6061 gaacgggatg ctgctggccg gtgaagtgga tgcgattctg gtcgcgcgcc cgcccgatgc
     6121 gttcctcgcg ggcgatcccg atatcgtgcg cttcttcggg gatttcctgg aggtcgagca
     6181 gcgctattac aaggagacgg gcatttttcc aatcatgcat acggtggcgg tgcgccgtga
     6241 cgtgctcgac aagaatccct gggtcgcgcg caatcttttt tacgccttcg acgaggcacg
     6301 caaacgcgcc atcgcgcgcg cgctcgacaa cacggtttcg ctctatccga tcccctgggg
     6361 tttcgaatat gcgcgccgtg gcctcgacat gatgggagac gattatttcc cctatggcat
     6421 ggagaaaaac cgcaagaccc tggaggcttt cctcggctac gcccatgagc agggcgtctg
     6481 caagcgcctg ctcaggatcg aggaattgtt tcccaagcag ttcctcagcg tgcacaaagt
     6541 ctagccgcgc ggggtcctaa agacctatat agggcgtgag atccggtgac ggtgtacgcg
     6601 atggatagcc acgcgcaatt gcgcaagaac gccgatgagt tcgcacgcta ttatggccgg
     6661 cttgcgaagg tgaacatggc gcccctctgg gaggtgatgc gcaatctcgt cacgcccgag
     6721 cccgtgtcgc cggtgcaacc ggcgcagtgg cgctatgccg acgtgcggcc gctgctgttc
     6781 gaatcggccg ccatgatcac tgccgagcag gccgagcggc gcgtgctggt gctggaaaac
     6841 cccgggcttc ccaaacaatc cgcgatcacg cattcgctct atgccggcat ccagcttgtc
     6901 atgcctgggg aaatcgcgcc gcagcaccgc catacccagg cggctcttcg cttcatcctg
     6961 gaaggaaggg gcgccaccac cacggtcaat ggcgagcgtt tcgagatgtt tccgggcgac
     7021 atgatcctga cgccgtcctg gcagtggcac gatcatcaga acgagaccga ggtgccgatc
     7081 gtctggctgg acggactcga tattccgctc gtcggcctgc tcgattccag ttttgcgcaa
     7141 acctccaacc gcaaggagca ggaggtcacg cgcgaaaaag aaaccagcca gaccatgtac
     7201 ggcgccaatc tgcttcccat cgacgacgcg ccggtctcgc gcaattcgcc gctgatgcgc
     7261 tacccttacg acaccgcgcg cgccaccctg gaacggatga agatctcgtc gccgctcgat
     7321 ccctgccacg gcatccgcat gcgctacgcc catccgcgca ccggcgattg ggcgatgccg
     7381 accatcggtc cggcgattca gcttgtgccg aaagggctcg ctaccacacc ctaccgcgcc
     7441 accgacggaa cgatcttctc ggtcgccgaa gggcagggca cggcgaaaat cggcaatgcc
     7501 gagtttgcgc tttcgccgcg cgatgtgttt gtctgtccgc cctgggcatg gcgcagcttc
     7561 aaggcggacg acgatctggt gctttttaat ttctccgatc gtccggtaca ggaaaagctc
     7621 ggtctctggc gcgaagaacg cactccatcc gtcgcgtaaa tggacagcct gaagacccgt
     7681 gccgatccca tcctgattgc cggcggcggc atcggcggac tcgccgctgc cctcgcgctg
     7741 gctcggcgcg gattcccgtc ccgcgtgctg gagcaggccc ccgagttcaa ggaggttggg
     7801 gccggcattc agctcggtcc caattcctgg cgttgcttac gcgcgcttgg cgtcgaagcg
     7861 gcggtgaagc ggctggcggt tttccctgag gcgctcgtga tgatggatgg ggtgacggcg
     7921 gagccggtgg tttccatccc gcttggcggt tttgagacgc gcttcaaggc gccctatgcg
     7981 ctgattcatc gcgcggattt gcacctgtgc ttgctcgaag cctgcaaggc gtcttctcat
     8041 atcgcgctcg aaacctcatg cctcgtgacc gatttcaccg atggtggcgc gaatgttcgc
     8101 gtcgcgaccg gcgacagaaa tttccgcgac acgccggcgc tcatcgccgc ggacggtctg
     8161 tggtcggcgg cgcgcagcaa actgatcggc gacggcagcc cgcgcgtgtc cgggcatatc
     8221 agctatcgct cggtgctcaa gccggccgat gtgccggaga atctgcgccg caacgacatg
     8281 gttctctggg ccggtccgaa atgccacatg gtgcattatc cgctgcgggg gggcgagttg
     8341 ttcaacctgg tcgcgacctt ccacagcgat cgctacgagg aaggctggaa ttccttcggc
     8401 gatcccgccg aactgcacgc gcgatttagc ggcacctgcg acatcgtgca aaccatgctc
     8461 ggccgcatcg aggaatggcg catgtgggtc ttgtgcgacc gcgagccgat tgccgattgg
     8521 agcaaggggc gggtgacatt gctcggcgat gccgcccatc cgatgctgca atatctggcg
     8581 caaggggca
//
LOCUS       DASYEG010000202         4963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_62357, whole genome shotgun sequence.
ACCESSION   DASYEG010000202 DASYEG010000000
VERSION     DASYEG010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62357"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VGM72_09115"
     CDS             complement(<1..460)
                     /locus_tag="VGM72_09115"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF013575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HEY4265466.1"
                     /translation="MPRYRVTLEYDGGPFAGWQRQENGPSVQGALEEAIFRLASERVT
                     VTGAGRTDAGVHARGQVAHFDLVKEFAPATIRDALNAHLRPAPIAIVDAALPATDFHA
                     RFSAIGRHYEYRILTRRAPPALDRGRVWHVGRPLDLAAMCEGARAFIGQHD"
     gene            complement(460..1374)
                     /gene="fmt"
                     /locus_tag="VGM72_09120"
     CDS             complement(460..1374)
                     /gene="fmt"
                     /locus_tag="VGM72_09120"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY4265467.1"
                     /translation="MSLRLAFMGTPDFAVPTLAALIGAGHAIACVYSQPPRPKGRGMT
                     AGKSPVHIFAETSGIAVRTPVSLKGGEEQVAFTGLELDAAVVVAYGLLLPRPVLDAPR
                     LGCFNLHGSLLPRWRGAAPIQRAIMACDSETGVMAMRMEEGLDTGPVLMAERVAIGRK
                     TYGELHDELARLGADLMVRALGAVERGSVTPVPQAEDGATYAKKIEKSETRIDWSKSA
                     HELDCMIRGLSPAPGAWFEAKGERIKILLAGTGPGKGAPGDVTFDFAIVCGESALRPL
                     LVQRAGRAPADWLSFLRGFALKPGERVV"
     gene            complement(1402..1914)
                     /gene="def"
                     /locus_tag="VGM72_09125"
     CDS             complement(1402..1914)
                     /gene="def"
                     /locus_tag="VGM72_09125"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY4265468.1"
                     /translation="MALLELVKAPDPRLKLVSEPVPEVDSKLRRFMTDMVETMYAENG
                     IGLAAIQVGVAKRVAVIDLDPGGANSKPIYLVNPRIVEASGALSTYHEGCLSVPEVWD
                     DVKRPAELTVEYTDEHGKLQRVKADGLFATCLQHEIDHINGLLFIDHLSKLKRSIALR
                     KSAKLKRLGD"
     gene            1999..3129
                     /gene="rmuC"
                     /locus_tag="VGM72_09130"
     CDS             1999..3129
                     /gene="rmuC"
                     /locus_tag="VGM72_09130"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEY4265469.1"
                     /translation="MGIWISSLAVIAVLTGLGLTAYALLRVGTALRQSERQRDAKDSG
                     ESLFLEAKLRELSAAQSEIAGRFSQAIESQAKSQSELQRAVAERLEALDKRLGDNLKD
                     TASKTAETLGGLQTRLTVIDEAQKNLADLSGQVVSLQQILANKQARGAWGQGQMENII
                     RDALPISVYEFQATLSNKNRPDCLIRMPGSASAIVIDSKFPLESFAVLKNAGSDAERR
                     LAEARVRADVGKHVRDIAEKYLIPGETQTPAIMFVPSESLYAELHDSFSDVIQKAQRA
                     QVVVVSPNILLLAINTVQTVMKDARMRDQANLIQKEVGLLLNDVRLLHDRVNKLQSHL
                     GQADGDIKNILISTAKVTGRAERIERVELAGPDHCAIATAAE"
     gene            3407..4369
                     /locus_tag="VGM72_09135"
     CDS             3407..4369
                     /locus_tag="VGM72_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY4265470.1"
                     /translation="MNKFMVAGLGVLCFSPVALPYLFATSDAFPSWAYPVDPPATTVA
                     HAPKDDGTLLHVPDSDVALTRTQIDGGDMVADWHPEDHPAMPDIVKKGRQNVRACAYC
                     HQPSGAGRPESASLAGLTEPYIKEQIDTFKKGLRKGSEPTRVPENMMNQIAANLTEDE
                     IAEAAKYFSGLTLGSFVKIVETDTAPKTFVTDGMLAKSPEGGTEPIGARIIEVPEDLA
                     RAKNRDPRAPFVAYVPKGSLQAGKPFVTGGALPCISCHGSDLHGRGDIPHIAGRSPSY
                     ILRQLYDIQKGNRTGAVAPMQQVVSGLKLNDMIAIAAYVASRDP"
     gene            4473..4829
                     /locus_tag="VGM72_09140"
     CDS             4473..4829
                     /locus_tag="VGM72_09140"
                     /inference="COORDINATES: protein motif:HMM:NF023204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HEY4265471.1"
                     /translation="MRTGVSIGFISIVAVIGLGAAPSFAGLPFPGKHSDVEKIQYDRY
                     PDQRRDDRNDRDYRGPKWRPGQVLPGQFLNRVVGDWEERGLSRPPSGHQWVRVGQQFI
                     LVRGSDRMIARILNFD"
     gene            complement(4839..>4963)
                     /locus_tag="VGM72_09145"
     CDS             complement(4839..>4963)
                     /locus_tag="VGM72_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEY4265472.1"
                     /translation="IENYFCRIKDWRRIATRYDKLARNYLAAAAIVGALVWIKL"
BASE COUNT          962 a         1592 c         1571 g          838 t
ORIGIN      
        1 aatcatgctg cccgatgaag gcccgcgcgc cttcgcacat ggccgcgagg tcgaggggcc
       61 tgcccacgtg ccagacgcgc cccctgtcca gcgccggcgg cgcgcgccgg gtgaggatgc
      121 gatattcgta gtgacggcca atcgctgaaa accgcgcatg aaaatcggtt gccgggagtg
      181 ccgcgtccac aatcgcgatg ggtgccgggc gcaaatgcgc attcagcgca tcgcgaatgg
      241 tggcgggcgc aaattccttc accagatcga aatgggcgac ctggccgcgc gcatgcacgc
      301 cggcatcggt gcggcctgct cccgttaccg taacgcgctc gctcgcgagc cgaaaaatcg
      361 cctcttccaa cgcgccctgg accgaagggc cattctcctg ccgctgccat cccgcgaacg
      421 gaccgccatc atattcgagc gtgacgcgat agcgcggcat cagacgaccc gctcgccggg
      481 tttgagcgcg aagccgcgca ggaaggagag ccaatcggcg ggcgcacggc ccgcgcgctg
      541 taccagaagc ggcctgagcg cactctcgcc gcagacaatg gcgaaatcga acgtcacatc
      601 gcccggcgcg ccctttcccg gtcctgtccc cgccagaaga atcttgatgc gctcgccctt
      661 ggcttcgaac caggcgcccg gcgcgggcga cagcccacgg atcatgcagt ccagttcgtg
      721 cgcggatttc gaccaatcga tgcgggtttc ggatttttcg atcttcttcg cataggtcgc
      781 gccgtcttcg gcttgcggca ccggtgtcac gctgccgcgt tccaccgcgc ccagcgcacg
      841 caccatgaga tcggcgccca accgcgcgag ttcgtcatgc agctcgccat aggtcttgcg
      901 gccgatggcg acccgctcgg ccatcagcac cgggccggtg tcgaggcctt cctccatccg
      961 catcgccatc acgccggttt cggaatcgca cgccatgatc gcgcgctgga tgggggcggc
     1021 tccgcgccag cgcggcagca gcgagccgtg cagattgaaa cagccaaggc gcggtgcatc
     1081 cagaacgggg cgcggcagca gcagcccata ggccaccacg accgcggcat ccagttccag
     1141 gccagtgaac gcgacctgct cttcgccacc cttcagcgac accggcgtgc ggacggcaat
     1201 cccggacgtt tcggcgaata tatgaaccgg cgattttcct gcggtcatgc cacggccctt
     1261 ggggcgcggc ggctggctgt agacgcaggc gatcgcgtgg ccggccccaa tcagggccgc
     1321 cagcgtcggc acggcgaaat ccggtgtccc catgaaggcg aggcgcaagc tcatggcggc
     1381 aagcctagca cggaggacaa ctcaatcgcc caggcgcttg agcttggccg atttgcgcag
     1441 cgcgatggag cgtttgagct tggagagatg gtcgatgaac agcaggccgt tgatgtggtc
     1501 gatctcgtgc tgcagacagg tggcgaacaa cccgtccgct ttcacgcgct ggagcttgcc
     1561 atgctcgtcg gtgtattcga cggtcagttc ggcggggcgt ttgacgtcat cccagacctc
     1621 cggcaccgac aggcagccct catgataggt ggatagcgcg cccgacgcct ccacgatgcg
     1681 cggattgacc agatagatcg gcttgctgtt ggcgccgccg gggtcaaggt cgatcaccgc
     1741 cacgcgcttg gccaccccca cttggatggc ggcgagtccg atgccgtttt cggcatacat
     1801 ggtctccacc atgtccgtca tgaatcgcct gagcttggaa tcgacctccg gcacgggttc
     1861 ggagaccagc ttcaaccggg ggtcgggtgc cttgacgagt tcgagcagag ccatgggcgc
     1921 cggagatatg tgaaggctcg gggcgggtca agcgcgcgcg aatcacctaa gatgcccccg
     1981 gaacataagg tgaatgccat ggggatttgg atttcgagtc tggcggttat cgccgtgctg
     2041 acgggactgg ggctcaccgc ctatgcgctc ctgcgggtgg ggacggcgct gcgccaatcc
     2101 gagcggcagc gcgacgccaa ggacagcggc gaaagcctct ttttggaagc caagctgcgc
     2161 gaactcagtg ccgcgcaaag cgaaatcgcc ggacgtttct cgcaggccat cgaaagccaa
     2221 gcgaaaagcc agagcgagtt gcagcgcgcg gtggccgagc ggctggaagc cctcgacaag
     2281 agacttggcg acaatctgaa ggacaccgcc tcgaagacgg ccgaaacctt aggcggcctg
     2341 cagacgcgcc tgaccgtgat cgacgaggcg caaaagaatc tcgccgatct ctccggacag
     2401 gtggtcagcc tgcagcagat ccttgccaac aagcaggccc gtggcgcctg gggccagggc
     2461 cagatggaga acatcatccg cgatgcgctc cccatcagcg tctacgaatt ccaggccacc
     2521 ctttccaaca agaaccggcc cgattgcctg atccgcatgc cgggctcggc gagcgccatc
     2581 gtcatcgatt cgaaattccc gctcgaatcc tttgcggtcc tgaagaacgc cggcagcgac
     2641 gcggaacggc gccttgccga agcgcgcgtg cgcgcggacg tcggcaagca tgtgcgcgac
     2701 atcgcggaga aatacctcat ccccggcgag acgcagacgc ccgccatcat gttcgtgccg
     2761 tccgaatcgc tttacgccga attgcacgat tcattttccg atgtgatcca gaaggcgcag
     2821 cgcgcacagg tggtcgtggt ctcgcccaat atcctgcttc tggccatcaa cacggttcag
     2881 accgtgatga aggacgcgcg catgcgcgat caggcgaacc tgattcagaa ggaagtagga
     2941 ttgctgctga acgatgtgcg gctgttgcac gaccgcgtga acaaattgca gagccatctc
     3001 ggccaggcgg acggcgacat caagaacatc ctgatctcga ccgccaaagt caccggccgc
     3061 gccgagcgca tcgaacgcgt ggaattggcg ggcccggacc attgcgcgat cgccacagcc
     3121 gcggaatagc cggcgagagc acaccgccgg gaagcgcgag gccatcccag cgcggcgatt
     3181 tcgtcggcgc ccatcgcgtc ctgacggccg ccgccggcat ggcgggagag accgagatcg
     3241 cccgggcggc actgcgggaa ctccgccgcg ccgggctgga ttaaccccgt tcgcggaaaa
     3301 agagccgtcc gtgcctgcga ccgcaaacgg ccttgcaacg ctacgcgaaa tcctcattct
     3361 cgcgcgttgc gaatttctcg accgtcgtcc gcgtggaggg gccttaatga acaagtttat
     3421 ggttgccggg ctgggcgttc tctgtttctc cccggtggcg cttccgtatc tcttcgccac
     3481 cagcgacgca tttccgagct gggcctatcc cgtcgatcca ccggcaacga ccgtggcgca
     3541 cgcgccgaaa gatgacggca ccctgctcca tgttcccgac agcgacgtcg cgctgacgcg
     3601 cacccagatc gatggcggcg acatggtggc cgactggcat cccgaggatc acccggcgat
     3661 gcccgacatc gtcaagaagg ggcggcagaa tgtgcgcgcc tgtgcctatt gccatcagcc
     3721 gagcggtgcg ggacggcccg agagcgcatc gctcgccggg ctcacggaac cttacatcaa
     3781 ggaacagatc gacaccttca agaagggcct gcgcaagggc tccgagccca cgcgtgtgcc
     3841 cgaaaacatg atgaaccaga tcgcggcgaa cctgaccgag gacgagatcg cggaggcggc
     3901 gaaatatttt tccggcctga cgctcggctc cttcgtgaaa atcgtcgaga ccgatacggc
     3961 gcccaagacg ttcgtcaccg acgggatgct ggcgaaatcg cccgagggcg gcaccgaacc
     4021 tatcggagcg cgcatcatcg aagtgccgga agatctggcg cgggcgaaga atcgcgatcc
     4081 gcgcgcgccg tttgtcgctt acgtgccgaa gggaagcctg caggcgggca agccttttgt
     4141 caccggcggc gccctgccct gcataagctg tcacggcagc gatctgcacg gacgcggtga
     4201 tattccccat atcgcggggc gttcgccatc ctatattctg cgccagctct acgacattca
     4261 gaaaggcaac cggaccggag ccgttgcccc gatgcagcag gtcgtatccg gtctcaagct
     4321 caacgacatg attgcgatcg cggcctatgt ggcaagccgc gatccctgag ctggagtgcc
     4381 ccgcgcccaa cagtcatcgc gtggcatggc ggaacgcagc caaaacagat agattatgtc
     4441 ccgtaagatc agacgttctg cgggaggaac ctatgagaac tggcgtttcg ataggattca
     4501 tttcgatcgt cgcggtcatt gggcttggtg ccgcgccgtc attcgcgggg ctgccgttcc
     4561 ccggcaagca cagcgatgtg gagaaaatcc aatatgatcg ttatcccgat cagcgacgcg
     4621 acgatcgcaa cgatcgggat taccgtggcc cgaaatggcg tccgggacag gtgcttcccg
     4681 gccagttcct caatcgcgtg gtcggcgatt gggaagaaag aggattaagc cggcccccta
     4741 gcggccatca gtgggtccgg gttggccagc agttcatact cgtcagagga agcgatcgga
     4801 tgatcgcccg gatcctcaat ttcgactagg gtctggactc acaacttgat ccagacgagc
     4861 gcgccgacga tggcagcggc ggcaaggtaa tttctggcga gtttgtcata ccgggttgca
     4921 atgcgcctcc aatccttgat gcggcagaag tagttctcga tct
//
LOCUS       DASYEG010000203         3908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_62528, whole genome shotgun sequence.
ACCESSION   DASYEG010000203 DASYEG010000000
VERSION     DASYEG010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62528"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VGM72_09150"
     CDS             <1..1050
                     /locus_tag="VGM72_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265473.1"
                     /translation="IVCGAIALLLPKGIPTLQQAMLVTILMATINQPHFAHSYQMFYR
                     NFREKAFGASYPHALRIRYILAGLAVPAALLAFLAGTITSGDVRMLATGANLMFFLVG
                     WHYVKQGYGILIVDSVQKRLMFPDRAKTILRLNGYACWLVAWVGANHALSQANDYIGL
                     TYFSLPIPNAVYDLSIAAAVVTTLAALYALGGHWRANKGALPWNGIVAYLTTLYFWVI
                     FVRVNPLVLAVIPTFHSLQYLAVVWRYQLNAGAKPAPARSLWNAFWPSGVLPNLAMFI
                     GVGVLLGFIGFIGAPRVLDAVLPYDKHLFGPSLFLFSFYIFINVHHYFLDNVMWRRGN
                     PDVQQYIFSRPNPAR"
     gene            1134..3176
                     /locus_tag="VGM72_09155"
     CDS             1134..3176
                     /locus_tag="VGM72_09155"
                     /inference="COORDINATES: protein motif:HMM:NF009379.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY4265474.1"
                     /translation="MPRKNGGSGPRCVALVGPYGAGKTTLLESILWVTGAIQRKGSVP
                     QRNTVGDGSPEARSRQMSVEINAANTRYMEETFTVLDCPGSIEFLQETLNVLPGVDAA
                     IVVCEPEAGKAAMLQPYLKRLADLNIPHALFVNKIDKASGAIRDLLATLQEVAPKPLV
                     LRQIPIWENDIATGFVDLALERAYVYREHAASELIDMNNSVKAREAEARFQMLEKLAD
                     YDEHLMEELLSDIQPARDAIFEDLQRDLQQGLIVPVLLGSAERDNGVRRLLKMLRHDV
                     PDVSEVCARAGVSGNDTIVHVLKTYHTSHGGKLSLARVLTGHLKDGAVLYRRGGADTR
                     VGGLFALRGETQLKLNEANAGDTIALGRLEEVMTGEALSSAKSGVKVKSKAETLTPVY
                     DLAITAHDRKDEVKLTGAIHKLQEEDPSLHFRQNAELQEMSLEGQGEIHLKVAAEKLF
                     TKYGLKIDTHRTKIPYKETIKKPGRQHGRHKRQSGGHGQFGDVYLEIKPMPRGTGFIF
                     EDLIKGGVVPRNFIPSVEKGIVEFLKEGPLGFPVVDLAVALVDGSYHTVDSSDAAFQT
                     AARIGMTEGMPNCAPVLLEPIMRVKIHVPSGSTADVNSVISTRRGRILGFDAREGWRG
                     WDTVEAEIPQAELHDLIVELRSLTQGVGTYEMSFDHLAELSGKLAENVVTARKAAA"
     gene            3268..3768
                     /locus_tag="VGM72_09160"
     CDS             3268..3768
                     /locus_tag="VGM72_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005247040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HEY4265475.1"
                     /translation="MDMDIGISTTHREAIAEGLSRVLADTYVLYLKTHAYHWNVTGPL
                     FSSLHLLFETQYNELHDAGDTIAERIRALGHFAPGSYANFARLTSIAEDNDSVPGALS
                     MVRNLASGHEALGRTLRAAFKTAEAGGDQATMDLLIGRMEASDKTGWMLRSHLENPSS
                     ETMPKL"
     gene            complement(3784..>3908)
                     /locus_tag="VGM72_09165"
     CDS             complement(3784..>3908)
                     /locus_tag="VGM72_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEY4265476.1"
                     /translation="IENYFCRIKDWRRIATRYDKLARNYLAAAAIVGALVWIKL"
BASE COUNT          768 a         1138 c         1172 g          830 t
ORIGIN      
        1 attgtctgcg gggcaatcgc gctgctcctg cccaagggta tccccaccct gcagcaggcg
       61 atgctggtga cgattctgat ggcgacgatc aaccagcccc atttcgcgca ttcctatcag
      121 atgttctacc gcaatttccg cgagaaggcg ttcggtgcgt cttacccgca cgcattgcga
      181 atccggtaca tcctagccgg tctcgccgtc cccgccgcgc ttctggcatt tctcgccggg
      241 acgatcacca gtggcgatgt acgcatgctg gccactggcg ccaatctgat gtttttcctg
      301 gtcggctggc attatgtgaa acaaggctac ggcatcctca tcgtggattc cgtgcaaaag
      361 cggctgatgt ttccggaccg cgcaaagacg attttgcggc tgaacggtta tgcctgctgg
      421 ctggttgcgt gggtcggcgc aaatcacgcg ctctcacaag ccaatgatta tatcggtctg
      481 acctatttct cgcttcctat ccccaatgcg gtttatgatc tctccatcgc ggcggcggtg
      541 gtgaccacgc ttgcggccct ttacgcgctc ggcggacact ggcgcgcgaa caaaggcgcg
      601 cttccctgga atggcatcgt cgcctatctg accacgctgt atttctgggt tatcttcgtg
      661 cgcgtcaatc cgcttgtgct ggcggtcatt ccgaccttcc attcgctgca atatctggcg
      721 gtggtctggc gctatcagct caatgccggc gcgaagccag cgcctgcgcg ttccctctgg
      781 aacgcattct ggcctagcgg cgtgctcccc aatctcgcca tgttcatcgg cgtgggcgtg
      841 cttctgggct tcatcggttt catcggcgcg ccgcgcgtcc tggacgcggt cctgccctat
      901 gacaagcatc tgtttggccc cagcctgttt cttttcagct tttatatctt catcaacgtc
      961 catcattatt ttctcgacaa tgtgatgtgg cgccggggaa atcccgacgt gcagcaatat
     1021 attttctccc gcccgaaccc cgcccggtaa accggcaagt tttttcagac tggcttgcct
     1081 tcccccccgg attggcgcga gcataggcgc cgtcggtctc aagggaggcg aatatgccga
     1141 gaaaaaatgg aggaagcggg ccacgctgcg tggccctggt cggaccgtac ggtgcaggaa
     1201 aaaccacgct tctcgaaagc attctctggg taaccggcgc tattcagcga aaaggttccg
     1261 tgccccagcg caacacggtg ggcgatggtt cccctgaagc ccgttcgcgt cagatgagcg
     1321 tcgagatcaa tgccgcgaac acgcgctaca tggaggagac cttcaccgtt ctcgactgtc
     1381 cgggctcgat cgaattcctg caggagacac tcaacgtgct tcccggcgtc gatgcggcca
     1441 tcgtggtgtg tgaacccgaa gccggaaagg ccgcgatgct tcagccctat ctaaaacggc
     1501 ttgccgatct gaacatcccg cacgcgctgt tcgtcaacaa gatcgacaag gcgtcaggcg
     1561 ccattcgcga tctccttgcg accctccagg aggtggctcc caagcctctc gtgctgcgtc
     1621 agattccgat ctgggaaaac gacatcgcga ccggctttgt cgatcttgcg ttggagcgcg
     1681 cctatgttta tcgcgagcac gctgcttccg aactgatcga catgaataat tcggtgaagg
     1741 cacgcgaggc cgaagcgcgc tttcagatgc tcgagaaact cgccgattac gacgagcatc
     1801 ttatggaaga gctgctctcc gacatccagc cggcgcgcga tgcgatcttc gaggatctgc
     1861 agcgcgactt gcagcagggt ctgattgtgc cggttctgct cggctcggcg gagcgcgaca
     1921 atggcgtcag gcggcttctg aagatgctgc gccatgacgt ccctgacgtt tcggaagttt
     1981 gtgcgcgcgc cggcgtttcc ggcaacgata caattgtgca cgtgctgaag acctatcaca
     2041 cctcccatgg cggcaagctc agtcttgcgc gcgtgctgac gggccatttg aaggacggcg
     2101 cggttctgta tcgccggggc ggcgccgata cgcgtgtggg gggtctcttc gctctgcgcg
     2161 gcgaaacgca actgaagctc aacgaagcga atgccggcga caccattgcg cttggccgtc
     2221 tcgaagaggt gatgacgggc gaggcgctgt cgagcgcgaa atccggcgtc aaagtgaaga
     2281 gcaaggccga gacgttgacg cccgtttacg atctcgccat cacggcgcac gatcgaaagg
     2341 acgaggtgaa gctcaccgga gctattcaca aattgcagga ggaggatcct tcgctgcatt
     2401 tccgccagaa cgcggagttg caggaaatgt cgctggaagg acagggcgag attcatctga
     2461 aagtcgcggc cgaaaaactg ttcaccaaat atggtctgaa gatcgacacg caccgcacga
     2521 agattcctta caaggagacg atcaagaagc cgggcagaca gcacggacgg cacaagcgcc
     2581 agtccggcgg ccatgggcag ttcggcgatg tgtatctcga gatcaaaccg atgccgcgcg
     2641 gtacgggttt tatcttcgag gatctgatca agggcggcgt ggtgccgcgg aatttcattc
     2701 cctcggtcga gaaaggcatc gtggaatttc tcaaggaagg gccgctggga tttccggtgg
     2761 tcgaccttgc ggtggccctc gttgacggct cttaccacac ggtggattcc tcggatgcgg
     2821 ccttccagac ggcggcgcgc attggcatga ccgaaggcat gcccaattgt gcgcccgtgc
     2881 tgctcgaacc gatcatgcgc gtgaagatcc acgtgccatc ggggtcgacc gcggatgtca
     2941 acagcgtgat ctcgacgcgg cggggccgca ttctcggttt cgacgcacgc gaaggctggc
     3001 gcggctggga tacggtcgag gcggagattc cgcaggcgga acttcatgac ctgatcgtgg
     3061 agttgcgttc gctcacgcaa ggtgtgggga cctatgaaat gagcttcgac catctggcgg
     3121 aattaagcgg caaattggcg gagaacgtgg tgactgcacg gaaggccgcc gcataaagcg
     3181 acaggctccg cccttgaata tagtttagaa ttattctaat atcttcggag gaccgagtgg
     3241 ttccggccga ctggatggag agcccttatg gatatggata tcggaatctc gacgacccat
     3301 cgcgaggcca tcgcggaggg tctatcgcgc gtactcgcgg acacctacgt gctctatctc
     3361 aaaacccacg catatcattg gaatgtgacg ggcccgctgt tctcgtctct gcatctgttg
     3421 ttcgagaccc aatacaatga gctgcacgat gcgggcgaca ccattgcgga gcgtattcgc
     3481 gcgcttgggc actttgcacc cggcagctat gccaatttcg cgcgcctgac cagcattgcc
     3541 gaagacaatg acagcgtgcc gggagccctc agcatggtgc gcaatctcgc ctcgggccac
     3601 gaagcactgg gacgaacctt gcgcgcggct ttcaagacgg ccgaagccgg cggcgatcaa
     3661 gcgaccatgg atcttttgat cggccgcatg gaggccagtg acaagactgg ctggatgttg
     3721 cgcagtcatc tggagaatcc gtcctccgag acgatgccga agctgtaagc ctagggtctg
     3781 gactcacaac ttgatccaga cgagcgcgcc gacgatggca gcggcggcaa ggtaatttct
     3841 ggcgagtttg tcataccggg ttgcaatgcg cctccaatcc ttgatgcggc agaagtagtt
     3901 ctcgatct
//
LOCUS       DASYEG010000204         1653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4702813, whole genome shotgun sequence.
ACCESSION   DASYEG010000204 DASYEG010000000
VERSION     DASYEG010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4702813"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="VGM72_09170"
     CDS             complement(<1..857)
                     /locus_tag="VGM72_09170"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4265477.1"
                     /translation="MGTIDADAHVIETMTTFDYIDPEYLHLKPRIVRQVEGEHARSNE
                     GGIQKEFWIIDGRLQPKEHNVGSNTSDVSREMRDVQARLDHMDALDIEVQVLYPTVFL
                     RAWTQDPTVEVVLCRSYNRWLADIWKQGKGRLRWVVMPPLRSMDETRKEIAFGKENGA
                     CGIFVRGLEAEMRLANPYFYKLYEMAQEFDLPVCFHSGNNSFAVRDIYATEGGFARSK
                     LPVVGAFHNIVMDDLPGKFPKLRWGFIEVSAEWLPYAFNDLELRFKRKDRKFPTDPLK
                     TNNMYVACQ"
     gene            complement(932..1306)
                     /locus_tag="VGM72_09175"
     CDS             complement(932..1306)
                     /locus_tag="VGM72_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265478.1"
                     /translation="MVLIALIASITAICYFAFALYLLLLPFVVPPAAALLTGILVLVV
                     ALLLIAIARTAFRPRRARREPGPSAEAAEDAAGLGTEIGRKVRGLADAHGSGGLLAAL
                     VAGFAVGVSPKLRAFLQALLKP"
     gene            complement(1353..>1653)
                     /locus_tag="VGM72_09180"
     CDS             complement(1353..>1653)
                     /locus_tag="VGM72_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265479.1"
                     /translation="IMNDYVAGSDTDGDVQALRAELRNLRADFSKITELLKDSARHRG
                     AEAADKIRASAEQGWNEAKSTAQSVIEEMEERPLATVGAVFVAGLLFGLMLGGRR"
BASE COUNT          330 a          511 c          508 g          304 t
ORIGIN      
        1 cactgacagg cgacatacat attgttcgtc ttcagcggat cggtggggaa tttccggtcc
       61 ttgcgcttga agcgcagttc cagatcgttg aacgcatagg gcagccattc ggcgctcact
      121 tcgatgaaac cccagcgcag cttggggaat ttcccgggca ggtcgtccat gacgatattg
      181 tggaaggccc ccaccaccgg cagcttggag cgggcaaagc cgccctcggt cgcatagatg
      241 tcgcgcaccg cgaagctgtt gttaccggag tggaaacaaa ccggcagatc gaattcctgc
      301 gccatctcgt agagcttgta gaaataggga ttggccaggc gcatctcggc ttccagcccg
      361 cgcacgaaaa tcccgcaggc gccgttttcc ttgccgaagg cgatctcctt gcgggtctca
      421 tccatggacc gcaagggcgg catgaccacc cagcgcagac gcccctttcc ctgcttccag
      481 atgtcggcga gccagcgatt gtaactgcgg cagagcacaa cttcgacggt ggggtcctgc
      541 gtccaggcgc gcaggaaaac cgtgggatag agcacctgca cttcgatatc gagcgcgtcc
      601 atgtggtcga ggcgcgcctg cacgtcgcgc atctcgcgcg aaacgtccga ggtgttggag
      661 ccgacattgt gctccttggg ttgcagccgc ccgtcgatga tccagaattc cttctggatc
      721 ccgccctcat tgctgcgggc atgctcgccc tcgacctggc ggacgatgcg cggcttcaaa
      781 tggaggtatt ccggatcgat ataatcgaac gtcgtcatcg tttcgatgac gtgtgcatcg
      841 gcgtcaatgg ttcccatgtc ttcctcgaac ggcgttttat cgtttgagga acaatagcgg
      901 cgttatcggg ccgcgcacaa tgcggcgcta ttcagggctt gaggagggct tgcaggaaag
      961 cccggagctt gggactcaca cccaccgcga aaccggccac cagggcggcg agaagcccgc
     1021 cgctgccatg ggcatcggca agaccacgga ccttgcggcc gatctcggtg cccagaccgg
     1081 cggcatcttc ggcagcttcc gcggaagggc ccggttcacg tcttgcccgg cgcggacgaa
     1141 acgcggtgcg ggcgatggcg atcagcagca gcgccaccac aaggacgagg attccagtca
     1201 gcaacgccgc ggccggcgga accacgaacg gcagcagcag cagatacagc gcgaacgcga
     1261 aatagcagat cgcggtgatg gacgcgatca gggcgatcaa aaccatcgcc agcgaaatcg
     1321 cgaggcgggc gaagaaacgg cttatcacag cgctaccggc ggccgccgag cataagcccg
     1381 aacaataggc ccgcgacgaa caccgctccg accgtggcaa gcggacgttc ttccatttct
     1441 tcgatgacgc tctgcgccgt gctcttggcc tcgttccagc cctgttcggc gctggcgcga
     1501 attttgtcgg ccgcctctgc cccgcgatgg cgggcggaat ctttcaagag ctcggtgatc
     1561 ttggaaaaat cggcgcgcaa attccgcaat tcggcgcgaa gcgcctgaac gtcgccgtct
     1621 gtgtctgagc ccgcaacata gtcgttcatg ata
//
LOCUS       DASYEG010000205         2154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1221823, whole genome shotgun sequence.
ACCESSION   DASYEG010000205 DASYEG010000000
VERSION     DASYEG010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221823"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VGM72_09185"
     CDS             complement(<1..374)
                     /locus_tag="VGM72_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY4265480.1"
                     /translation="MARAEATAASENEAGLRPVDGRELSQNVLERLKKFYRDMLLIRR
                     FEEKAGQLYGMGLIGGFCHLYIGQEAVVVGMQATLKPEDQVITTYRDHGHMLACGMEP
                     AGVMAELTGRSTGYSHGKGGSM"
     gene            complement(483..794)
                     /locus_tag="VGM72_09190"
     CDS             complement(483..794)
                     /locus_tag="VGM72_09190"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEY4265481.1"
                     /translation="MNRRIGRNIRLIVLPAICCAMSGYFGYTVVFGERGLIAWRQTQD
                     QLRIAQSDLASLKGKREALEHRISLLDGKAIDPDLLEEVARGELLETRTGEVAVPREK
                     R"
     gene            complement(877..1353)
                     /locus_tag="VGM72_09195"
     CDS             complement(877..1353)
                     /locus_tag="VGM72_09195"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY4265482.1"
                     /translation="MTSKLAALVAKDVALRKTRSTYPEKFAARLGGRTKRPLGDAFGL
                     TIFGVNLTTLPPGAMSALRHAHTRQDEFVYILEGTPFLITDSGETLLQPGMCAGFKCG
                     TGDGHHLVNRSQIDVVYLEVGDRTLGDEASYPDDDIVAIKTADGSRKFTAKDGTPY"
     gene            complement(1429..>2154)
                     /locus_tag="VGM72_09200"
     CDS             complement(1429..>2154)
                     /locus_tag="VGM72_09200"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265483.1"
                     /translation="VMMLGRKPEAADEGAHPLEHPNPPLPALDGLFRQVTDMTWDPAG
                     NIYISDGYINSRVAKYDKDGNWLMQWGDRGSEPGKLNTPHSIASDAQGNIYVADRGNG
                     RIQVFKGTGQLQRVMKIDVTAPPGSKALIGNTPTPNASGGYGNVATMSPGSPWALCIT
                     PGPNQVLYSSDAFPGRIYKLTLDGKVLGMIGGGGRTAKKFGWIHEIACPNENTLYVGE
                     LLNWRVQKITLHPTNQQRAENNR"
BASE COUNT          376 a          694 c          643 g          441 t
ORIGIN      
        1 tgcatcgagc cgcccttgcc gtgggaatag ccggtggagc ggcccgtcag ttccgccatc
       61 acgcccgccg gttccatgcc gcaggccagc atatggccgt gatcgcgata ggtggtgatc
      121 acctggtctt caggcttcag cgttgcctgc atgccaacca ccaccgcctc ctggccgatg
      181 tagaggtggc agaacccgcc gatcagcccc atcccgtaaa gctgcccggc cttctcctcg
      241 aaacgccgaa tcaacagcat gtcgcgatag aatttcttca gacgctcgag cacgttctgg
      301 gacagctcgc gcccgtccac cggccgaagt ccggcctcgt tctcactggc cgccgtcgct
      361 tccgcgcgtg ccatatctca ccctgtggct ctcatcgtta cgaccgcgcc cccgggtgcg
      421 gccaaccgct tatggcaagg gtgaattgcc cttggcaagg cggaacttaa ctcctggaat
      481 tcctagcgct tttcgcgcgg caccgctacc tcgccggtcc gggtctcaag cagctcgccg
      541 cgggcgacct cctcaagcag atcgggatca atggccttgc cgtcaaggag cgagatgcga
      601 tgctccagcg cctcacgctt gcccttgagc gaggccaggt cgctctgggc aattcggagc
      661 tggtcctgcg tctgacgcca ggcaataagg ccacgctcgc caaaaaccac ggtgtagccg
      721 aaataaccgc tcatcgcgca gcagatcgca ggcaacacaa tgaggcggat attccgtccg
      781 atgcggcgat tcattcgcat ggacgtcacg gaatcacatc ccgattcccc gggcaagaaa
      841 attcatcgtt ggtttagaaa ttgtttcagc gggcgctcaa tatggcgtgc cgtccttggc
      901 ggtgaatttt cgcgagccgt cggcggtctt gatcgccacg atatcgtcat cgggatagga
      961 cgcctcgtcg ccgagcgtgc ggtcgccgac ctccagatag acgacgtcga tctggcttcg
     1021 gttcaccagg tgatgtccgt cgccggttcc gcatttgaac cctgcgcaca tgccgggttg
     1081 aagcagcgtc tcgcccgaat cggtgatgag gaagggcgtg ccttccagga tatagacgaa
     1141 ctcatcttgt ctcgtatgcg cgtggcgcag tgccgacatc gcgcccggcg gcagggtcgt
     1201 caggttcacg ccgaagatgg tcagtccgaa cgcatcgccg agcggacgtt tcgtgcggcc
     1261 tcccagccgc gccgcgaatt tctccggata ggtgctgcgg gttttacgca gagccacatc
     1321 ctttgccacc aatgccgcca gcttggacgt catgactcac cctttcatca aagaaaaggc
     1381 caggacgcgc acgcgtcctg gccttcatca catagcacag acgctgctct agcgattgtt
     1441 ctccgcgcgc tgctgattgg tgggatgcag cgtgatcttc tgcacccgcc agttcagaag
     1501 ctccccgacg tagagcgtgt tttcgttcgg gcaggcgatc tcgtggatcc agccgaattt
     1561 cttggccgtg cggccgccgc cgccgatcat gccgagcacc ttgccatcga gggtcagctt
     1621 gtagatgcgc cccggaaacg catccgaact gtaaagcacc tgattcggtc cgggagtgat
     1681 gcagagcgcc cagggcgaac cggggctcat cgtcgcgaca ttgccatagc cgcccgaggc
     1741 gttgggtgtc ggcgtgttgc cgataagcgc cttgctgccc gggggcgccg tcacgtcgat
     1801 cttcatcacc cgctgcaatt ggcccgtgcc cttgaacact tggatgcgtc cattgccgcg
     1861 gtcggcgacg tagatgttgc cctgcgcgtc ggaggctatc gagtggggtg tattcaactt
     1921 ccccggctcg ctgccgcggt cgccccattg catcagccaa ttgccgtcct tgtcgtattt
     1981 ggcgacgcgc gaattgatgt agccatcact gatatagata ttgccggcgg ggtcccacgt
     2041 catgtccgtg acctgacgga acagcccgtc gagcgcgggc agcggtggat tgggatgttc
     2101 gagcggatgg gcgccttcgt ccgcagcctc gggcttgcgg cccagcatca tcac
//
LOCUS       DASYEG010000206         4502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2095642, whole genome shotgun sequence.
ACCESSION   DASYEG010000206 DASYEG010000000
VERSION     DASYEG010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2095642"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VGM72_09205"
     CDS             <1..805
                     /locus_tag="VGM72_09205"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4265484.1"
                     /translation="ALWDRLLEPLLLAALNTRPEIGSAKLVAAVLRETLAKGGKAYLP
                     RIASPTLAAALVDPAVALIEGRGGVFQFGQRLRSIGFGASGAEVLELSGEDVALTAHD
                     RIIVAVPPWVAKPLLPGIETPDEYRAILSGHFGITPPQGAAPMTGLIGADVEWIFTFP
                     DRISVTISDADRFNGTGRAELAEMCWRDVARVFHLPTELPPWQIVKEKRATFAATPAQ
                     ERKRPGSATPWPNLFLAGDWTATGLPATIEGAVRSGKRAAQLVLSSMAG"
     gene            814..2766
                     /gene="shc"
                     /locus_tag="VGM72_09210"
     CDS             814..2766
                     /gene="shc"
                     /locus_tag="VGM72_09210"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEY4265485.1"
                     /translation="MDDFSRAPVSQPYLDAAIAGATDALLREQRSDGHWVFELEADAT
                     IPAEYVLLAHYLGEAADLELERKIAVYLRRIQGAHGGWPLFHGGPLDVSATVKAYFAL
                     KMIGDAPDTPHMTRARAALLARGGAENANVFTRFLLALYGECPWSAVPTVPVELILLP
                     RWFPVHLSKMSYWARTVLVPLLVLAALKPRARNSRGIHVEELFRGQTISAQRRAAHQN
                     RYWAAFFGALDHVLKWVEPFWPKRLRRRAVDACVAFVGERLNGEDGLGAIYPAMANSV
                     MMFDALNYPPDHPQRAIARAACANLVVVRDTEAYCQPCVSPVWDTALVCHTLLEVGGA
                     EAEARAEHGLEWLEPRQILDVKGDWAEERPTTRPGGWAFQYNNAHYPDLDDTAVVVMG
                     FDRAKTAGATTRDRQEAIARGREWVVGLQSKNGGWGAFDADNCHHYLNNIPFADHGAL
                     LDPPTADVSARCVGMLAQLGASASDPEMATGIAYLKGVQEKDGSWFGRWGVNYIYGTW
                     SALAALNAAGLQSGDSAVRRAADWLIAIQNEDGGWGEGCDSYRLDYRIYQEAPSTASQ
                     TAWALLGLMAAGEVDHAAVARGVAFLAKTQTADGLWQQEHYTGGGFPRVFYLRYHGYP
                     KFFPLWALARYRNLKTGNSRRVAYGL"
     gene            2763..3452
                     /locus_tag="VGM72_09215"
     CDS             2763..3452
                     /locus_tag="VGM72_09215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265486.1"
                     /translation="MTIIAVSGLLRESRIASGPGIVTLAAGGNAALLRTRLEQTIAQG
                     ARGIISFGIAGGLSPSLKPGDCIIGVEVRDGEQRFISDEAWTARLAARLPDAILAPFA
                     GADAILASKSAKADVFGQTGAHAVDMESHVVARLAEAHRLPFTVLRTIADPAGSALPP
                     LARVAITAEGRMDLVAILKSLVGEPGQIGLLMRTARDSKAAFGSLLRCRRALGLGLLG
                     PDGRELALDMG"
     gene            complement(3375..>4502)
                     /gene="hpnH"
                     /locus_tag="VGM72_09220"
     CDS             complement(3375..>4502)
                     /gene="hpnH"
                     /locus_tag="VGM72_09220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HEY4265487.1"
                     /translation="AVRVGSYVLKQHLSRRKRYPLVLMLEPLFRCNLACAGCGKIDYP
                     DDILNQRLSVAECMKAIDECGAPVISIAGGEPLLHREMPEIVKGYLARKKIVILCTNA
                     LLLAKKIDLYTPHPDFNWSIHLDGDKEMHDASVCQKDVYEKAVDAIKLAKAKGFRVSI
                     NCTLFNTAEPARVAAFFDEMKRIGLDGITVSPGYAYERAPDQEHFLNREKTKQLFRGI
                     LSRGRGGKSWSFTQSAMFLDFLAGNQSYHCTPWGNPTRTVFGWQRPCYLLGEGYVETF
                     KELMEGTDWDRYGVGNYEKCANCMVHCGFEASAVVDSIKRPLTALAVTLRGVRTDGPM
                     AADIPLDKQRPAEFVFSTHVERKLATIRAQKTKPESATAAE"
BASE COUNT          768 a         1390 c         1490 g          854 t
ORIGIN      
        1 tgcgctttgg gaccggctgc tggagccctt gctgctggcc gccctcaata cgaggcccga
       61 gatcggaagt gcgaaactcg tggcggcggt tttgcgcgag accctggcga agggcggcaa
      121 ggcctatctg ccgcgcatcg cttcgcccac actggcggcc gcgctcgtcg atcctgccgt
      181 ggcccttatc gagggacgcg gcggggtgtt tcagttcggc cagcgtctca ggagcatcgg
      241 ttttggagca tccggcgccg aagtgctgga actttccggg gaggacgtgg ccctcacggc
      301 gcatgaccga atcatcgttg ctgtgccgcc ctgggtggcg aagccattgc tgcccggcat
      361 cgagacaccc gacgaatacc gcgcgattct gagcggacat ttcggcatca cgccgccgca
      421 aggcgcagcc cccatgacgg ggctcatcgg tgcggatgtc gaatggatat tcacctttcc
      481 cgaccggatt tccgtcacca tcagcgacgc cgaccgcttc aacggaaccg gccgcgcgga
      541 attggcggaa atgtgctggc gcgatgtcgc gcgcgtgttc cacttgccga cggaattgcc
      601 gccctggcag atcgtcaagg aaaagcgtgc gaccttcgcc gccacgcccg cgcaggaacg
      661 caagcggccc ggaagcgcga cgccatggcc caatctgttt ctggccggcg actggaccgc
      721 taccggactt ccggccacca tcgaaggtgc tgtgcgttcg ggcaaacggg cagcccaact
      781 ggtgctttcg agcatggctg gatagaaagc ggcatggacg atttttcccg tgcgcccgtc
      841 agtcagccct acctcgatgc ggctatcgcc ggggcgacgg atgcgctgtt gcgcgagcag
      901 cggagcgatg ggcattgggt gttcgagctc gaagccgatg cgaccattcc ggcggaatat
      961 gtcttgctcg cccattacct cggcgaagcg gccgatctcg aactggagcg caagatcgct
     1021 gtctatctgc ggcgcatcca gggcgcccat ggcggctggc cattgtttca tggcgggccc
     1081 ttggatgtca gcgcgaccgt caaggcctat ttcgccctga agatgattgg cgatgcgccg
     1141 gacaccccgc acatgacgcg cgcgcgcgcg gcgctcctgg cgcgtggcgg cgcggaaaac
     1201 gccaatgtgt tcacgcggtt tcttctggcc ctctatgggg agtgcccgtg gagcgcggtg
     1261 ccgacggtgc cggtggagct gattctgctg ccgcgctggt ttcccgtgca tctgtcgaaa
     1321 atgtcctatt gggcgcgaac cgtgcttgtg ccgctcttgg tgctggcggc cctcaagcct
     1381 agagcgcgca attcgcgtgg tatccatgtg gaagaattgt tccgtgggca gaccatatcg
     1441 gcgcagcgcc gcgcggccca tcaaaaccgc tattgggcgg cattcttcgg cgcgctggat
     1501 catgtgctga aatgggtcga accgttctgg ccgaaacgcc tgcgccgccg cgccgtcgat
     1561 gcgtgcgtcg cgttcgtcgg cgaaaggctg aacggcgagg acgggctggg cgcgatctac
     1621 ccggcgatgg ccaacagcgt gatgatgttc gatgcgctga attatccgcc ggatcatccg
     1681 cagcgcgcca tcgcgcgggc cgcatgcgcc aatctcgtgg tcgtcaggga tacggaagcc
     1741 tattgccagc cttgcgtctc gccggtgtgg gataccgctc ttgtctgtca cacgctcttg
     1801 gaagtgggcg gagcggaggc tgaagcacgc gccgagcatg gcctggaatg gctcgaaccg
     1861 cgccagattc tggacgtgaa aggcgattgg gcggaggagc gtccaaccac gcgtcccggc
     1921 ggctgggcgt ttcaatacaa caacgcgcat tatcccgacc tcgacgatac cgccgtggtc
     1981 gtgatgggtt ttgaccgcgc caagacagcg ggtgccacga cgcgcgaccg gcaagaggcc
     2041 atcgcgcgcg ggcgtgaatg ggtcgtgggt ctgcaaagca agaatggcgg ctggggcgcc
     2101 ttcgatgccg acaattgcca ccattatctc aacaacatcc cgtttgccga tcatggcgcg
     2161 ctgctcgatc cgcccaccgc cgatgtcagc gcgcgctgtg tcggcatgct ggcgcaattg
     2221 ggcgcgagcg cgagcgatcc cgaaatggcc acgggcatcg cctatctgaa aggcgtgcag
     2281 gaaaaggacg gaagctggtt cggacgctgg ggtgtgaact atatctacgg cacctggtcc
     2341 gcgcttgcgg ccctgaacgc ggccggactt caatccggcg attccgccgt gcggcgcgcc
     2401 gcggattggc tgatcgccat ccagaacgaa gacggcggct ggggcgaggg ttgcgacagc
     2461 taccggctgg actaccggat ttatcaggag gcaccttcga ccgcatcgca gacggcctgg
     2521 gcgctgctcg gactgatggc ggcgggcgag gtggatcatg cggcggtcgc gcgcggtgtc
     2581 gcgttcctgg caaaaacgca aaccgccgat gggctgtggc agcaggaaca ctacacgggc
     2641 ggcgggtttc cgcgcgtgtt ctatttgcgc tatcacggct atcccaaatt ttttccgctc
     2701 tgggcgctgg cgcgctatcg caatctcaaa accggcaatt cccgccgggt ggcttacggg
     2761 ctgtgacgat catcgccgtc agcgggttgc tgcgcgaatc ccgtatcgcg agcggccccg
     2821 gcatcgtgac gctggcggcc ggcggcaatg ccgcgctgct gcgcacgaga ctggagcaga
     2881 ccatcgcgca aggcgcgcgt ggaatcatca gcttcgggat cgccggcggc ctttcgccgt
     2941 cgttgaagcc cggcgactgc atcatcggcg ttgaagtccg cgatggagag cagcgcttca
     3001 tttccgatga agcatggacg gcgcgccttg ccgcgcgcct gccggacgcc atcctcgcgc
     3061 cgttcgcggg tgcggatgcg atcctggcgt cgaaatcggc aaaagcggat gtgtttggcc
     3121 aaaccggagc ccatgccgtc gatatggagt cccatgtcgt ggcccggctg gcggaagcac
     3181 accggcttcc cttcaccgtg ctgcgcacga ttgccgatcc cgccggcagc gcgctgcctc
     3241 cgctcgcccg cgtcgccatc accgccgagg ggcggatgga tcttgtcgcg attctcaaat
     3301 cgctggtggg cgaaccgggg cagattgggc tgctgatgcg gacggcgcgc gactcgaagg
     3361 ccgcgttcgg ttcgctactc cgctgccgtc gcgctctcgg gcttggtctt ttgggccctg
     3421 atggtcgcga gcttgcgctc gacatgggtt gagaagacga attcggccgg acgctgtttg
     3481 tcgagcggaa tgtccgcggc catcggcccg tccgtgcgca cgccgcgcaa agtgacggcg
     3541 agcgcggtca agggacgttt gatgctgtcc acgacggcgg acgcctcgaa gccgcaatgg
     3601 accatgcagt tcgcgcattt ctcgtaattg ccgacgccgt aacgatccca gtccgttcct
     3661 tccatcaact ccttgaaggt ttcgacatag ccttcgccca acaaatagca gggccgttgc
     3721 cagccgaaca ccgtgcgtgt cggattgccc cagggcgtgc agtgatagct ctgattaccg
     3781 gcgaggaaat ccaggaacat cgccgattgc gtgaacgacc agctctttcc gccacggccg
     3841 cgcgaaagga tgccacgaaa aagctgcttg gtcttttcgc gattgaggaa atgttcctga
     3901 tcgggcgcgc gctcataggc atagcccggc gacaccgtga tcccgtccaa tccgatgcgc
     3961 ttcatttcgt cgaagaacgc cgccacgcgc gcgggttccg cggtattgaa cagcgtgcag
     4021 tttatgctga cgcgaaaacc cttcgccttg gccagtttga tggcgtcgac ggctttttcg
     4081 tagacgtcct tctggcacac cgaggcgtcg tgcatctctt tgtcgccgtc gaggtggatg
     4141 gaccagttga aatcgggatg gggcgtgtac agatcgatct tcttggcgag gagcaacgca
     4201 ttggtgcaga gaatgacgat cttcttgcgg gcgaggtagc ctttcacgat ctcaggcatc
     4261 tcgcgatgca gcagcggctc acccccggcg atcgaaatca ccggggcacc gcattcgtcg
     4321 atggccttca tgcactcggc aacgctgagg cgctggttca ggatgtcatc ggggtaatcg
     4381 atcttgccgc agcccgcgca tgcgaggttg cagcggaaca gcggctccag catgagcacg
     4441 agcgggtaac gcttgcgccg gctcaaatgc tgtttgagaa cgtaactgcc gacgcgaacc
     4501 gc
//
LOCUS       DASYEG010000207        23235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4704060, whole genome shotgun sequence.
ACCESSION   DASYEG010000207 DASYEG010000000
VERSION     DASYEG010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 23235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23235
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4704060"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..136)
                     /locus_tag="VGM72_09225"
     tRNA            complement(60..136)
                     /locus_tag="VGM72_09225"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(100..102),aa:His,seq:gtg)
     gene            complement(288..1349)
                     /locus_tag="VGM72_09230"
     CDS             complement(288..1349)
                     /locus_tag="VGM72_09230"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEY4265488.1"
                     /translation="MKELLLSTPFHARTAEHNRANVWVPSDAYTVPAHYGDSRRESLA
                     ARCTAALIDVSATQDLSIAGEGAAALLSAVCGPAVRGIAIGHSQILHWCADRGGLRGF
                     GVMSRLAEDGFLLRSTDADIGWFAAAAARFQTRVRDATAERGLLLLTGPYAVAVMVAA
                     RLEILPLEAMRHGSFDWRGIAVRVFHRAHPDGYEISVAPEDAALAFDRLTRAGRLVSL
                     RLAGEDALQLLQLEAGVPLAYRDFAPAREAFACAPSPAALGFTASEGMGEGAGALVLA
                     GLELESDRPMPFAPVVAGPRDAGRTLRSLYSPSLKAAIALAELAPQHAAPGTAVTVRR
                     GERFSEVDVRARVVALPFL"
     gene            complement(1346..2899)
                     /locus_tag="VGM72_09235"
     CDS             complement(1346..2899)
                     /locus_tag="VGM72_09235"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY4265489.1"
                     /translation="MMMRRGGGDSYDAVIVGGGIGGLIAAAYLARGKARVLLLEAGDR
                     FGGRAETLEFADGFHAPLMSHVAYALDGRAMRELRLAEHGLEFAQTNMQFAALGPGGK
                     HFVLPGPGLRGSATLAAEAGDYATFRQKAMGFARLLRPLWDGRLADPGIENRDDALAA
                     IVRGLQLGLRKSEGFEILLRLSAAAFLDGWLESDMLKAALSFDVFPSGLSPHEAGSAL
                     VLMWRFAQESCGRQGAVSQIRGGPGALARALEAAARQAGAELRPSSHVSAIIVEGRRA
                     KGVTLTEGETIPAAMVLSSLDSQDTFALVEPVSIGFATGTRGRAPRRTASAQVLFALK
                     GAPPFAGLTSEHFGARLVIAGRPEIASEARGTALNGGVSSELAMEVTIPTAADPALAP
                     AGCHVLSALVPYMPAAVAGGWEVPRMMLHRQVLATLEEFAPGLRNRLLACRVIAPGDE
                     DARGSACDSASCTGRLLASYEARIRTPIAGLYMCGRSAEPVSAISGRAGRLAANLAAM
                     GDHRMGALL"
     gene            complement(2896..4434)
                     /locus_tag="VGM72_09240"
     CDS             complement(2896..4434)
                     /locus_tag="VGM72_09240"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY4265490.1"
                     /translation="MDHFDVAVIGAGPEGLIAAIVLARTGLRVVLLEKASAPGGRAAS
                     QEFHPGFRASVYADELPDIPNRLYRSLDLARHGAILEPSPASVCISNGGASVLFVDDA
                     RLAHSLPAAALPGILALRREVETLRLAIEARAALPSHAPRRRWFGSVRKSVGAVPWPA
                     GGWAGASLDEVLGAHIADPQLRLHLAADAVSGRAVSPFLAGTALHALAPGGGRSGLAP
                     GGLGRLAIALARAAESAGVVMRCASEVSDIHVERGRATTLSVSGQGPVAAAAIVSALD
                     LKRTFLGLLRWDAMPVPLAGRIGRFRVAGQRARVLFALDRPPAIPVLSTIEDAAAGAI
                     HVAESMQALSVAHDIWRAGAVPSDPLVTLRVPSFADPRFAPIGKAVMTATLSPIPTRL
                     FDGGWTHERRAKLAARALAAAKRVMPDIEALVLAHRIIAGPDIETALGATDGDCDGGE
                     LAPDQVLGFRPFHDAQWQDGRTPVSGLYLGGASSAASPFLLGVSGERAALAVAADFAA
                     GGLR"
     gene            complement(4602..5081)
                     /gene="speD"
                     /locus_tag="VGM72_09245"
     CDS             complement(4602..5081)
                     /gene="speD"
                     /locus_tag="VGM72_09245"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HEY4265491.1"
                     /translation="MAQLGLGVAAINGARALKARVPARTVPVPIPEGDADRKDHFVTK
                     NGLVFAGSHLIIDLWEAEGLDDKDRIEQAMIDAVEAAGATLLHIHLHTFTPSGGVSGV
                     AVLAESHISVHTWPERGYAAFDIFMCGNAEPRKALAVLKQAFSPKRVVLGEHKRGVL"
     gene            5412..6287
                     /gene="speE"
                     /locus_tag="VGM72_09250"
     CDS             5412..6287
                     /gene="speE"
                     /locus_tag="VGM72_09250"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEY4265492.1"
                     /translation="MFRERLHKGYAQSMELTAVLADEKSQYQQIRIFDTVANGRVMVL
                     DDIVQITTRDESAYAEMLTHLPLLEHGSASRVMIVGGGDLSIADEALKHKSVKEVVLV
                     DIDGRVVELCRKHFGAFNAKAFKDKRLKIEIADAFEYLGRPDSKARFDLIVADRPDPV
                     GPGKALFGDNFYARVRKALRPNGFATFQTGVPFYQPWEITEALNDLAAYFPHSGLYLT
                     VVPSYIGGFMALSWAGMGKPLGTPAGIRRAGARFATAKLKTDYYNPEIHAAAFAFPEW
                     VKRLVPKKRGRRSKV"
     gene            complement(6277..7830)
                     /locus_tag="VGM72_09255"
     CDS             complement(6277..7830)
                     /locus_tag="VGM72_09255"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEY4265493.1"
                     /translation="MALDPRIAEAWDRLPEYLGAHVELSLGALALGLMLSLPLAFFGA
                     RNPRARWLMLTVASVIQTIPSLALLALFYPLLLGLSTLTKAVTGAQFSALGFLPSLLA
                     LTLYSMLPILRNTVIGLLGVDAAIKEAALGVGMTPMQSLLRVELPLALPVIMAGVRTA
                     SVLVIGTATLSTPVGQTSLGNYIFTGLQTQNWVFVLFGCVAAAGLALIADALLALLER
                     LFARRGRPLLAGALVGSFLILSVGAFVPLMSRPAQYVIGAKGFPEQYILASLIDQRLT
                     AAGLTATTSSDLGSAVIFNALVAGDVDVYVDYSGTIWTNSMHRTDTPGREAMLREMGD
                     WLKRTNGARLLGPLGFENAYVLAMRRDRAAALGIHSIADLASVSRRLAVGGDYEIFAR
                     PEWRKLRDDYGVAFREERQMQPLFMYKAVADGDVDVITAFSSDGRIAEYDLVTLTDPK
                     QAIPPYDAVLLLAPKRAADTALAAALQPLVGRISIARMRQANLSVSRETGQVPPAEAA
                     RQLWNAIKP"
     gene            complement(7833..8702)
                     /locus_tag="VGM72_09260"
     CDS             complement(7833..8702)
                     /locus_tag="VGM72_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4265494.1"
                     /translation="MPFRQKLVSWLVLVAVLENRASQGAMMETGVCAIAIEDVWKSFD
                     GGSSFAVRGVTLRIARQEFVALIGTSGSGKTTLLKLINRLVEPTRGTVAVDGKPVAAA
                     DPVRLRRGIGYVFQGVGLFPHMTVAENIAVTLRLLEWDKAAIEARVDEMLDLVRLPRD
                     LGSRLPATLSGGQGQRVGVARALAAKPSIMLMDEPFGAVDPITRDALARDYRDLHETL
                     GLTTLMVTHDVLEAVLLADRIAVMETGELVECGPTQELLANAKRPSVRALMAMPRRQA
                     ERIGALAANSAGP"
     gene            8821..9693
                     /locus_tag="VGM72_09265"
     CDS             8821..9693
                     /locus_tag="VGM72_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265495.1"
                     /translation="MLQVSSSPDSAVFDAFFAGYDRAFILPDEKEDQAGFRACLALNA
                     EAAYARTAAGFGPFREVVLTAHEENTGALIGGANFVSFPLPSRIDGFKETLLSTNLNY
                     IYIDSQARRRGHFRRLVGALDDAARLLFESEAGRTPALAHLIFFELNDPFRLSPDSYE
                     RDTRHSGLDQFARVAIWAKLGARILDFSYVQPALSADQNPDEGLAYGLLGTSGAALSS
                     CVLHDHLERFFGISVFKGKSPLHDLAARAQLDGLRERCAQRAPIALLDPKTCPSQPDN
                     RFSSLRECLRASRA"
     gene            complement(9730..10890)
                     /locus_tag="VGM72_09270"
     CDS             complement(9730..10890)
                     /locus_tag="VGM72_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265496.1"
                     /translation="MARNAPWEVFPRETPMMDLEAVKLVVDGGALGFGAAELFRRAEQ
                     QVGPFLKSGVTNSMAYLTVDRGIMRLQKAGKGTLAAHTLKKLLVSEKRGSYFDHLLVI
                     EGVFFASALFSYGWWQRKDPSLVLRKDRLSRDRKSDQARKRLQPLQHFLFNGFEQWAP
                     SWDVNNWRENNHNLVGQIGTNDEADSIPVIVQDSAAAVNIREALSDKLVANATVTGLL
                     CHSSRAEKYLQPTELALVKAMRARAKEEDRDEQFYFLLVQDPDEGHRVTFSTAKVDYY
                     TGYVWQCWVPNEWMPERRFEAQIDDAFFVWQHANLADRDTVNLRMDGLHTDVKNIRKM
                     LVSHGLSGKMTLLQHLMPEQNIRDTKASGQAGKTTAEKDILSFDDFNKMVQR"
     gene            complement(11028..11924)
                     /locus_tag="VGM72_09275"
     CDS             complement(11028..11924)
                     /locus_tag="VGM72_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265497.1"
                     /translation="MAYNKHLFKITKKSVITAWAAWSGTAAFISAALSLALNNKIPAR
                     YSAELPFIVFIAIAAASATVWMSIYFWQRYRASQFWVPEGEVKEYIGRPYQADGDDLA
                     ELSKFASQVFGGDTMRAEVVRDVVGKRAALGVRLTDIGGKNIGFFDVFHLRPDALLDW
                     LEGKLQEPGMDGSHFEPIPKPLGDDAILELIVGAIYIAPQYSKSETGLAVAFAECCQR
                     YLWQRCAGFSEIVLYASIFKPEGERLARIYDFENYKVGGNRAGPAKKHDVLKRVLSID
                     DPIKVRHGLGGTDHFTIAMRAR"
     gene            complement(11975..14896)
                     /locus_tag="VGM72_09280"
     CDS             complement(11975..14896)
                     /locus_tag="VGM72_09280"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF010706.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HEY4265498.1"
                     /translation="MMPSGFNAPSNLPPLSDPGRAARTLADLGGAAPDALAEPGFRAI
                     LEAAAGNSPYLARLMLREQAFLPELLQDGPRNCLARLNAEAGAVAHDAGMTSVMRRLR
                     IAKRRAALAIALADIGGLFDLEDVTGTLTQFADACVKGGVGFLLASAARMQGLEERPP
                     GDLADSTGLVVLAMGKMGAFELNYSSDIDLVVFYEDERFPFARGGDKRTAAVDLVKNF
                     LKVLAEPTTDGYVFRVDLRLRPDAGATQIAISTVAAELYYESMGQNWERAAWIKARQC
                     AGDASAGARFLKNMEPFVWRKNLDYAAIQDIHSIKRQIHSHAGHGRVAVAGHNIKLGR
                     GGIREIEFFAQTQQLILGGRERTLRDPATLGALAALRERGHISQAAEDDMSDAYRFLR
                     RIEHRLQMIEDQQTHTLPKSPDGIDWVARFAGFDDTGIFEEALRAKLTTVQSHYARLF
                     EREAPLTTQTGSLVFTGVEEDPETVANLARMGFERPTDISAVIRSWHHGRIRATRGAR
                     ARELLTQLIPALLEALARTADPHAAFIHFDRFLSGLPGGVQAFSMLLANPKLLDLIAE
                     IAGSAPKLADYLGRHAAVLDALVDPDFLTRTPTHDELQARFAEDLGRVLGYEAALDAA
                     RRFTKEETFRIGVQVIQGFSTAEEAGPAYAAVAETVICGLQEIVEREMAAAHGHVPGG
                     AFAVVALGKLGGREMTAASDLDLVFIYTHDDDAVSSDGPKPLATSAYFARQAQRFIAG
                     LTAMTAEGRLYEVDMRLRPSGNQGPVAVRFETFVAYHREQSWTWERMALTRARVLSGP
                     ADLQMRINDAIRMTLTHGVTDSTRLLADARTMREKLAAQFPARDIWDIKFAAGGLVDI
                     EFIAQILQLTRAPSQPGVLDQNTIAALEKLAGHGLVGLADAEALIDATRMEHGLTQVL
                     RIAVDGPFKPDTATRGLKLLLARAGDAPDFSALETRLAQTQSQVRAIFEKTLTSGVRA
                     R"
     gene            complement(14947..16383)
                     /locus_tag="VGM72_09285"
     CDS             complement(14947..16383)
                     /locus_tag="VGM72_09285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY4265499.1"
                     /translation="MRTPKILRSQAFRIVAIYLAIFAVSAIALVGFVYWNTALVLDRE
                     TDETIEAEITGLVEQYHSLGLPGLTDAIIGRSIRREQGIYLLANNNRRVIAGSLDGWP
                     QVSPAARGFVEFSYERRIGTATELRRARGKVFMLTQGFYLLVARDVEERRVLENLFKT
                     ALYWAAGLMVVLGLAGGLVISRNFLARLDIINRTSRQIMGGDLSRRVPVTPADDEFDD
                     LSGNLNRMLDRIERLLHGMREVSDNVAHDLRSPLNRLRNRLELAAMRQAPDSDTARDF
                     DAAVQETDRLIGTFNALLLIAEAEAGSVRETMRDFSLTEILEGVSELYGPLAEENQLR
                     FVVRKAQDAGLVHGNRNLISQALANLVDNAIKYTPAGGTVVVALENQAQGPALVVADN
                     GPGIPTEDRPRITERFVRLESSRNSPGTGLGLSLVAAVARMHEAELTFEDNHPGLRAM
                     LSFKRVAEEPRPVKIMPVRAESQREPVH"
     gene            complement(16370..17047)
                     /locus_tag="VGM72_09290"
     CDS             complement(16370..17047)
                     /locus_tag="VGM72_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4265500.1"
                     /translation="MRVLVVEDDLEAQRYLVKGLKEAGHVVDDAGDGEIGLTLALSRP
                     YDVAIVDRMLPKLDGLKLVAEMREERSMTPVLFLSALSDVDDRIKGLRAGGDDYLTKP
                     YAFAELLARLDALVRRRDPGSVKTKVSVGDLELDLLSRTAKRNGSGIELQPREFRLLE
                     YLMRHAGQIVTRTMLLENVWEYHFDPQTNVIDVHISRLRAKIDKGFDAPLLHTVRGAG
                     YMIRAHA"
     gene            complement(17182..18846)
                     /locus_tag="VGM72_09295"
     CDS             complement(17182..18846)
                     /locus_tag="VGM72_09295"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEY4265501.1"
                     /translation="MMTETPNPKSSPASKRNLRMGVAAATIVALSFGAGGFVFSHDSG
                     TANGYVPQQTHSYTQLPEDGLHTVAAVTARQAQPTPPRLLDQGAPFSFADLVEHVSPA
                     VVTVVVDRESAGPQVAGLDDVPAPFRDFFRQFGGDGQGQGQGQGRGGQGRNRPMQRSE
                     AMGSGFIIDPTGYIVTNNHVVEEGKKISVKLPSGREYEAHLVGADKDTDVALIKIDGV
                     KDLPIVALGDDRRLRVGDWVVAVGNPFGLGGTVTAGIVSSIGRDIGNGPYTDYLQIDA
                     PINQGNSGGPTFDLTGRVVGMNTAIFSPSGGSVGIGFAIPASIVKTIVDQLKDHGNVS
                     RGWLGVQIQSLTPDMAASLGAGNAKGAIVASVVDDSPAMKAGFKQGDVITSLNGTDVD
                     DNRDLTRKVAGLRAGEKADFSVLRDGQKHNVTAIIAPRPDQVASADKPANSNRGDRGN
                     NRQASTTSLGMELMPLNADTREQFKVDGKVNGVVVSSVDPNSEAADKGFRPGDVIVGI
                     GNKNVRNPADIEQGVADAKKAGRETVLLLVAGDQGQHYVALKVAKA"
     gene            complement(18961..19425)
                     /locus_tag="VGM72_09300"
     CDS             complement(18961..19425)
                     /locus_tag="VGM72_09300"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEY4265502.1"
                     /translation="MRVLAVFLVAFLSAAALAPVAGLGAEEQPGESTYVPPLADPVLE
                     ARAKALQRELRCLVCQGQSIDESNSALAADLRRLIRQQIQSGQSNDDIKNFLVARYGV
                     FVLMQPPVRQDTFFLWFGPGLLVLAGGAVIGVTVLRARRRLGTEPDDLDVPV"
     gene            complement(19435..21402)
                     /locus_tag="VGM72_09305"
     CDS             complement(19435..21402)
                     /locus_tag="VGM72_09305"
                     /inference="COORDINATES: protein motif:HMM:NF007691.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEY4265503.1"
                     /translation="MTGELGQLALCLALALSLVQAFSGFTGASRGSVRLMAMGRGAAL
                     GSFVFIALAFFTLMYAYVTSDFSVMAVAENSHTAKPLIYKISGVWGNHEGSMILWLLI
                     LGIYSAAIAMTQPTDNPANAELGARALGVQALIAVAFLVFVLLTSDPFERFYPPPFEG
                     NGLNPLLQDPGLAFHPPLLYLGYVGLSAAFSFAAAALLDKRNDAAWVRRARPFATAAW
                     TALTLGIAAGSWWAYYTLGWGGFWFWDPVENASLMPWLIATALIHSMMATERTGAFKS
                     WTLLLAIAAFSFSLIGTFLVRSGVLNSVHSFANDPRRGLFILMILFVAITAPLVLFAW
                     RAPKLSPGAPFDLVSRETGIMTNNLLLTASAATILTGTLYPLVLDAAEGIKISVGPPY
                     FTATVVPMLGLLALVMPLGPITLWRRGNWRTTLYALRYAMAAALVAGVALLAIARPAP
                     ALGLITVMLGVWLIAGSITDLARRAGSWRRVRLLPSGAWSVALAHAGLGVVALGCVGA
                     TMWRSESIRVLAPGQTMNIAGYTLRLDGTEKVQGPNYFADRAAITVMAGGKTVTVVHP
                     EKRSYPVEQTVTTNSSIRTTVVSDLYVVLGDARQNGGWVVSAYVFPAAPFIWLGAVIM
                     ALAGFIALGARVASKVRAGRIAAAAVSEPAE"
     gene            complement(21399..21863)
                     /gene="ccmE"
                     /locus_tag="VGM72_09310"
     CDS             complement(21399..21863)
                     /gene="ccmE"
                     /locus_tag="VGM72_09310"
                     /inference="COORDINATES: protein motif:HMM:NF009731.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEY4265504.1"
                     /translation="MTPKKQRRLAFAITLLLAGGGAAALAVYALQDNILFFYSPSDIA
                     AHPEKVPEGRAFRIGGLVEKGSVKRDRGLEVHFVVTDGKNAVPVDYSGVLPDLFREGQ
                     GVVAMGTRSNSGVFLAREVLAKHDEKYMPPEVVDALKRSGHWQEGQGGPGSS"
     gene            22022..>23235
                     /locus_tag="VGM72_09315"
     CDS             22022..>23235
                     /locus_tag="VGM72_09315"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY4265505.1"
                     /translation="MVLRGFVAFVVLAALAFFTFFWAHEERLTWFDTRPVLAAAAKYD
                     ATIIRDRFGVPHIFGRRDADAGFGLGYAHATDDFPTLQRAFLSARGRLATLDGVKAAE
                     SDYLVQLLGIWDAIGAHYETDLSPETRALLDGYSAGLNLYAAQHRGQVLPGFTPVRGQ
                     DVVALFMLRLPFSYGLENQLHALIAGGAGKVAPDVSQGRATAIAVAPSRSADGATRLL
                     VNPQGPFEGVLSWYEARIVSGEGWNLAGGLIPGSPVIMAGAGPGFGWGIAPNHPDLAD
                     VYTLETNPNDRYFYRFEGEWRRLQTREARMVARLWGPIRITFRREILRSVQGPVIRNA
                     RGLFAVHYAGQDDLKGVEAFFHLNKAQNFEDFTAVLASGGIPSLDFVYADKTGRIASL
                     YNAAFPQRADGY"
BASE COUNT         4631 a         7403 c         7053 g         4148 t
ORIGIN      
        1 aaaatccaga tgccacaacg agctttataa gaaaacgagc tttataagaa tttgtgattt
       61 ggtacagctg ggttgagttg aacaaccgac cttcggatcc acaatccgac gctctaacca
      121 actgagctac agctgcacgc gatgccgcat gacgcggctg gcgggaaact aggaggggaa
      181 tttgccaatt gcaagcctgt gcaaagcgcg gccgtcttgc aaggacgcaa tggctgaagg
      241 acaaggacca gatcgtcaac gcttgagcgg aaatccgcgt ggaagagtca gagaaacggc
      301 aacgccacca cgcgcgcgcg cacgtccacc tcgctgaatc gctccccccg ccgcaccgtg
      361 acggcggttc ccggggcggc gtgctgtggc gccagttcgg caagtgcaat ggcggccttc
      421 agggacggcg aatagagcga gcgcaaggtg cgtcccgcgt cccggggccc tgcgacaacc
      481 ggcgcgaagg gcatcggccg atcgctctcc aattccagac cggcgagcac caacgcgccg
      541 gcaccttcgc ccataccttc cgaagcggta aagccgagtg cggccggtga cggcgcgcac
      601 gcgaaagctt cgcgtgccgg cgcgaagtcg cgataggcga gcggcactcc ggcctcaagc
      661 tgcagaagct gcaatgcgtc ctcccccgcg agccgcaacg acaccagccg tccggcgcgg
      721 gtcagccggt cgaatgccag tgccgcgtct tcgggggcga cagagatttc ataaccgtcg
      781 ggatgcgcgc gatggaatac cctcaccgcg attccacgcc aatcgaaaga gccgtggcgc
      841 atagcttcga gcggcaggat ttccagacgc gctgcgacca tgacggcaac cgcgtaaggg
      901 cctgtgagca acagcagacc gcgctcggcg gtcgcatcgc gcacacgcgt ttgaaagcgc
      961 gcggcagccg ctgcaaacca gccgatgtcg gcatcggtgc tgcgcaacag gaaaccatct
     1021 tcggcaagac gcgacatgac gccaaagccg cgcagccctc cccgatcggc gcaccaatga
     1081 aggatttgcg aatgaccgat ggcaataccg cgcacggccg gcccgcagac cgccgacaga
     1141 agcgccgcag cgccttctcc cgcaatgctc agatcctgcg ttgccgaaac atcgatgaga
     1201 gccgcggtac atcgcgctgc cagcgattcc cgacgcgaat cgccataatg ggccggcacg
     1261 gtataagcat cgctcggcac ccacacattg gcgcgattgt gttccgcggt gcgggcgtgg
     1321 aacggcgtcg aaagcagcag ctctttcata agagggcgcc catcctatgg tcgcccatgg
     1381 cggccaggtt cgcagcgagg cgtcccgcac ggccggaaat cgcgctgacg ggttcggccg
     1441 aacgaccgca catataaagc ccggcgatgg gcgtgcggat gcgcgcttca tagctcgcga
     1501 ggagcctccc ggtacaggac gccgagtcgc aggcgcttcc gcgcgcatct tcatcaccgg
     1561 gcgctatcac gcggcacgcc aggaggcgat tgcgcaatcc gggcgcgaat tcttccaacg
     1621 tggcgagcac ctgcctatgc agcatcatgc ggggaacctc ccagcctccc gcgacagcgg
     1681 caggcatata agggacgagc gcggagagga catggcagcc cgccggtgcg agcgccggat
     1741 cggcggccgt ggggattgtc acctccatgg ccagttcgct ggaaacgccg ccgttcagcg
     1801 cagtgcccct tgcttccgat gcgatctccg ggcgtcccgc gatgaccagc cgcgcgccga
     1861 aatgttccga agtgagcccg gcgaatggcg gcgcgccctt caatgcgaac aagacctgag
     1921 cgctcgccgt tcttcggggt gcgcgcccgc gcgttccggt cgcgaagccg atcgaaacgg
     1981 gttccacaag agcgaatgtg tcttgcgaat ccaggctcga aagcaccatc gccgcgggaa
     2041 tggtttcgcc ttcggtcagg gtgaccccct tggcacggcg cccttccacg atgatggcgc
     2101 tgacatgcga ggatgggcgc agctcggcgc ccgcttgcct cgctgccgct tcgagcgcgc
     2161 gtgccaaggc accgggaccg ccgcggatct ggctcaccgc gccttggcgg ccgcagctct
     2221 cctgcgcaaa gcgccacatc agcacgaggg cggagcctgc ttcgtgggga gacaggccgg
     2281 aaggaaagac atcaaaggac aaggccgcct tgagcatgtc gctttcgagc cagccgtcga
     2341 gaaaagcagc cgcactcagg cgaagcagaa tttcgaatcc ttcggatttg cgcagcccga
     2401 gctgcaggcc gcggacaatc gcggcaagtg catcgtctct gttttcgatc ccgggatcgg
     2461 caagtcttcc atcccacaac ggacgcaaga ggcgagcgaa tcccatcgcc ttttgccgga
     2521 atgtcgcata gtcgccggcc tccgccgcca acgtcgcaga gccacgcaga cccggtcccg
     2581 gcaacacgaa atgcttgccg ccgggcccta aggccgcgaa ctgcatgttc gtttgcgcga
     2641 actccagacc atgttcggca aggcgcagct cccgcatcgc gcggccatcc agcgcatagg
     2701 cgacatggga catgagaggc gcgtgaaatc cgtcggcaaa ttccagcgtt tcggcgcggc
     2761 cgccaaatct gtcgccggct tcgagaagaa gaacgcgtgc tttgccgcgc gccagatagg
     2821 ccgcggcgat cagcccgccg attccgccgc cgacaatgac cgcatcatag ctgtcgcccc
     2881 ccccgcgccg catcatcatc gcaagcctcc ggccgcgaaa tcagcggcca cagcgagtgc
     2941 ggcgcgttct cccgaaacgc caagcagaaa tggggaggcc gccgaagacg ctccgcccag
     3001 ataaagcccg gagaccgggg tgcggccatc ctgccattgc gcgtcatgaa agggccgaaa
     3061 acccaagacc tgatcggggg ccagctcacc gccgtcacag tcgccgtccg tagcgcctag
     3121 cgccgtttcg atgtccggac cggcgatgat ccggtgggcg aggacaagcg cttctatgtc
     3181 cggcatgacg cgcttggccg cggcaagcgc cctcgcggcc agcttggcgc gtctttcatg
     3241 cgtccatccg ccgtcgaaca atcgcgtagg aatgggcgac agcgtcgcgg tcatcaccgc
     3301 tttgccaatg ggcgcgaagc gcggatcggc aaaggacggc acgcgaagcg tcaccagcgg
     3361 atcgctggga accgcacccg cgcgccagat gtcatgagcc accgacaatg cttgcatcga
     3421 ttcggcgacg tgaatggccc cggccgccgc gtcctcgatg gtgctcagga ccggaatagc
     3481 cggcggccta tccagcgcaa acagcacacg cgctctctga cccgcgacgc ggaaacgtcc
     3541 gatgcgcccg gcaagcggga ccggcatcgc atcccagcgc aacaggccga ggaatgtccg
     3601 cttcagatcg agggcggaca cgatggcggc ggcggcgacc ggcccttgcc ccgaaacgct
     3661 cagcgtggtt gcgcggcccc gctccacgtg aatatcgctg acctccgaag cgcagcgcat
     3721 cacgaccccg gctgactccg ccgcgcgcgc gagcgcgatt gccaagcggc caaggccacc
     3781 gggtgccagg ccggaacgtc cgccgcccgg cgcaagcgcg tgcaacgctg tgccggcaag
     3841 aaacggcgac accgcgcggc ccgacaccgc gtcggccgcg agatgcagcc gcagttgcgg
     3901 gtccgcaatg tgtgcaccca gaacttcatc caacgacgcc cccgcccagc cgcccgccgg
     3961 ccaaggcaca gctcctacgg atttgcgcac ggagccgaac caccggcggc gcggcgcgtg
     4021 cgagggcaag gcggcacggg cttcgatcgc cagccgcaga gtctcgacct ctcgccgcaa
     4081 ggccaggatg ccgggaagcg ccgcggccgg aaggctgtgg gccaatcgcg cgtcgtccac
     4141 gaaaagcacg ctagcgccac cgttcgaaat gcagaccgaa gccggtgacg gctcaagtat
     4201 ggcgccatga cgcgcgagat cgagactgcg ataaagccga tttgggatat cgggcaattc
     4261 atcggcatag acggatgcgc gaaaacccgg atggaactcc tggcttgccg cacggccgcc
     4321 cggagcgctt gccttttcaa ggagaacgac tcgcagcccc gtgcgcgcaa gcacgattgc
     4381 cgcaatcagc ccttcgggac cagccccgat gactgccacg tcgaaatgat ccatgtcccc
     4441 ctgacctcag gcgtgtgccc gaggctccgt tacccagatg tcatttcata tccgtgggaa
     4501 aggttaatgc gtgaaatgca aaacaggaag gatgctggtg catccttcct gttttgtggt
     4561 taacgcgcgt tcggtttccc gagacgttca atcggcagga cttagagtac gccgcgcttg
     4621 tgctcaccga gcacaacgcg ctttggtgaa aaagcctgct tcaacaccgc gagcgcttta
     4681 cggggttcgg cgttgccgca catgaagata tcgaacgcgg cataaccgcg ttcgggccat
     4741 gtatgaacgc tgatgtggct ttcggccaat acagcgacac cgcttacacc cccgctgggc
     4801 gtaaaggtat gcaggtgaat gtggaggagg gtcgctcctg ccgcttccac tgcatcgatc
     4861 attgcctgtt cgatacgatc cttgtcgtcg aggccttcgg cctcccaaag atcgatgatc
     4921 aggtgactgc ccgcaaatac caaaccgttt ttcgtgacga agtggtcctt gcgatccgca
     4981 tccccttcgg gaataggaac cgggacggtc ctcgccggaa cgcgagcctt gagggctctt
     5041 gctccattga tcgccgccac accgagtccg agttgagcca tcctggcacc tacccttcca
     5101 aagtggtgtt gacaccaaag cgcgccattg ctgccgcgcc atcccaagaa aaaagcagcg
     5161 ggcggaaggg taacttccgc aacacgccgc tttgccccgg caagccgaga cagggggaaa
     5221 tatagggccg ctttccccca gcgcaagaaa atttagcccc tatggcaaat tttcttcaca
     5281 cgctcgccac gaagcgacgg caagaaagac gcagcgttcg aagttacgcc tcggaacggt
     5341 ggacagcaaa gcgctcgcgc cctataggcg aagccccaga cgagaaaata aacggtagaa
     5401 acgtgaccaa gatgttccgt gaacggcttc acaagggtta tgcccagagc atggaactga
     5461 ccgctgttct ggccgatgaa aagagccaat atcagcagat tcggattttc gacacggtgg
     5521 cgaacggccg cgtcatggtg ctggacgaca tcgtccagat caccacccgc gacgaatccg
     5581 cctatgccga gatgctcacc catctgccgc tcctggagca tggcagcgcg tcgcgcgtca
     5641 tgatcgtggg cggcggcgat ctttcgattg ccgacgaggc gttgaagcac aagagcgtga
     5701 aggaggtcgt gctggtcgac atcgacgggc gcgtcgtgga gctttgccgg aagcatttcg
     5761 gcgctttcaa cgccaaggcg ttcaaagaca agcgcctcaa aatcgaaatc gccgacgctt
     5821 tcgaatatct tggccggccc gattccaaag cgcgattcga tctcatcgtg gcagaccgtc
     5881 ccgatcctgt cgggcccggc aaggctttgt tcggtgacaa tttttatgcg cgcgtgcgca
     5941 aagcgttgcg gccgaacggc ttcgccacct ttcagacggg cgtgccgttc taccagccct
     6001 gggagatcac cgaggcgctg aacgatctcg ccgcctattt tccgcattcg gggctctatc
     6061 tcaccgtggt gccgagctat atcggcggct tcatggcgct gtcctgggcc gggatgggca
     6121 aaccgctcgg cacgcccgcc ggcatccgcc gcgcgggcgc gcgctttgca acggcgaagc
     6181 tgaagacgga ttattacaat cctgaaatcc acgctgcggc ttttgccttt cccgaatggg
     6241 tcaagcgcct agtgccgaaa aagcgcggcc gccggtctaa ggtttgatcg cgttccaaag
     6301 ctggcgcgcc gcttccgccg gcggcacttg gccggtctcg cggctgaccg acaaattggc
     6361 ttggcgcatg cgcgcgatgg aaatgcgccc gacaagcggc tgcagcgcgg cggcaagtgc
     6421 ggtatccgcg gcacgcttgg gcgccagcag aagtacggca tcgtagggtg ggatggcctg
     6481 cttggggtcc gtgagggtca ccagatcgta ttcggcgatt ctgccgtcgc tggagaatgc
     6541 ggtgatcaca tcgacatcgc cgtcggccac cgccttgtac ataaagagcg gttgcatctg
     6601 gcgctcctcg cggaaggcca ctccgtaatc gtcgcgaagt ttgcgccatt ccggtcttgc
     6661 gaagatttca taatcgccac cgaccgccaa gcgccgggaa acgcttgcga gatcggcgat
     6721 gctgtggatg ccgagtgccg ccgcgcggtc gcgccgcatc gccagcacat aggcattctc
     6781 gaaccccagc gggccgagca accgggcgcc gtttgtccgc ttcagccagt ctcccatttc
     6841 acgaagcatc gcctcgcgtc ccggcgtgtc ggtgcggtgc atcgaattcg tccagatggt
     6901 gccggaataa tccacataga catccacatc gcccgccacg agcgcgttga aaatgacagc
     6961 cgagccaaga tcgctgcttg tggtggcggt gaggcccgcc gccgtcaggc gctgatcgat
     7021 cagggatgca aggatatatt gctcgggaaa gcctttggct ccgatgacat attgggccgg
     7081 acggctcatc agcggcacga atgctcccac gctgagaatg agaaacgagc ccaccagggc
     7141 gccggccagc aatggccggc cgcggcgcgc gaagagccgt tccagcagcg ccaggagtgc
     7201 atcggcgatg agcgcaagcc ccgctgccgc aacgcagccg aaaaggacaa aaacccagtt
     7261 ctgcgtctga agcccggtga agatgtaatt gccaaggctc gtctgcccca ccggtgtcga
     7321 aagcgtcgcg gtcccgatca ccagcaccga agccgttcga acacccgcca tgatgacagg
     7381 cagcgccagc ggcaactcga cacgcaacag cgattgcatc ggcgtcatgc cgacaccgag
     7441 cgcagcttcc ttgatggcgg cgtccacgcc taaaaggccg atcacagtgt tcctcaatat
     7501 cggcagcatg ctgtagaggg tgagtgccag cagcgaggga agaaaaccga gtgcggagaa
     7561 ttgcgccccg gtcaccgcct ttgtgagcgt ggaaagcccg agcagcagcg gatagaaaag
     7621 cgccaaaagc gccaggctcg ggatcgtctg gatcacgctc gcgacggtga gcatcagcca
     7681 gcgggcacgg ggattgcgcg cgccgaaaaa agcgagcgga aggctaagca tcaaacccag
     7741 cgcaagcgcg ccgagactga gctccacatg cgcgcccaga tattcgggca gccgatccca
     7801 ggcttccgcg atacgtggat cgagcgccac cgtcacggtc cggccgaatt cgcagccaat
     7861 gccccgatgc gttcggcttg gcggcgcggc atggccatga gcgcgcgcac ggagggacgc
     7921 ttggcgttcg ccaggagttc ctgcgtcggc ccgcactcga cgagctcgcc tgtttccatg
     7981 accgcgatgc ggtcggcgag cagaaccgcc tcaagcacgt catgggtgac catcaacgtg
     8041 gtcagaccga gggtctcatg caggtcgcgg taatcgcgcg ctagcgcatc gcgggtgatc
     8101 ggatcgaccg cgccgaaagg ctcatccatc agcatgatcg acggcttggc cgccagggcg
     8161 cgagcgactc cgacgcgttg gccctggcct ccggaaagcg tcgccggcag acgggagccg
     8221 aggtcacgcg gcagccggac gagatcgagc atttcatcga cgcgcgcctc gatggcggcc
     8281 ttgtcccatt cgagcaggcg cagggtgacc gcgatgttct ccgccaccgt catatgggga
     8341 aacagaccca cgccctggaa cacgtagccg atgccgcggc gaagccgaac cggatcggca
     8401 gcagcgacag gcttcccatc gaccgcaacc gtgccgcgcg tcggttcgac cagccggttg
     8461 atgagcttga gaagggtcgt cttgccggag ccggaggttc cgatcagcgc cacgaattcc
     8521 tgccgggcta tgcgaagcgt cacaccgcga acggcaaaac tcgagcctcc atcgaaggac
     8581 ttccacacgt cctcgatcgc aatggcgcaa acgccggtct ccatcatggc tccttggctt
     8641 gcacggtttt caaggaccgc aaccagcacc aaccaggaaa ccagcttttg ccggaacggc
     8701 atagccctta tgctgatttt ggcgcccgtc cctgtgctat acaggcctat gcatgatgag
     8761 gactttcgcc ggatcaggcc ccaagcgggc actcagtgga gcctgcgcag cggagggctg
     8821 atgctccagg tcagcagcag ccccgatagc gccgtcttcg acgcgttctt cgccggttac
     8881 gatcgcgcct tcattctgcc ggatgaaaaa gaagaccaag ccggctttcg cgcctgcctc
     8941 gccctgaacg cggaggcggc ctatgcgcgg acggccgcgg gtttcggccc tttccgggaa
     9001 gtcgtcctga cggcccacga ggagaacaca ggcgcgctga tcggcggtgc gaatttcgtc
     9061 agctttccgc tgcccagccg catcgacgga ttcaaggaaa cgctcctctc gaccaatctc
     9121 aattacatct atatcgattc gcaggcacga cggcgcggcc atttccgccg gctggtgggc
     9181 gcgctcgacg atgccgcaag acttctcttc gaaagcgagg cgggcaggac gccagccctg
     9241 gcgcatctga tcttcttcga gctgaacgat ccgtttcgtc tctcccccga cagctacgaa
     9301 cgcgataccc gccattcggg tctcgatcaa ttcgcgcgcg tcgcgatctg ggcaaaactc
     9361 ggcgcacgca ttttggactt ttcctatgtg caaccggcgc tttctgccga tcagaacccc
     9421 gacgagggcc tcgcctatgg cttgctcggc acaagtggcg cggcgctcag cagttgcgtg
     9481 cttcatgacc atcttgagcg ctttttcggg atatccgtgt tcaagggcaa aagccccctt
     9541 cacgacctcg cggctcgcgc gcagctcgac gggttgcgcg aacgctgcgc gcagagggcg
     9601 ccaattgcgc ttctcgatcc taaaacttgt ccgtcgcaac ccgataatcg tttttcctcg
     9661 ttgcgcgaat gtctacgagc gtccagggcg tgagagagtc cgaatattcc atcatcgcac
     9721 tccgggcaat cagcgctgca ccattttgtt gaagtcgtcg aagctcaaaa tatctttttc
     9781 ggcggtggtt ttgccggctt ggcctgacgc cttggtgtcg cggatatttt gctccggcat
     9841 gagatgctgc agcaaggtca tcttgccgct taggccgtgg ctgaccagca tcttccttat
     9901 gttcttcaca tcggtatgca acccgtccat gcgaagattc acggtgtccc tgtcggcaag
     9961 attggcgtgt tgccaaacga aaaatgcatc gtcgatctgt gcttcaaatc tacgctcggg
    10021 catccactca tttggaaccc agcactgcca gacgtaaccg gtataataat cgactttcgc
    10081 ggtcgaaaac gtcacgcgat gtccttcatc cggatcctgg acgagtagaa aatagaactg
    10141 ctcgtcccgg tcttcttcct ttgcacgggc gcgcatcgcc ttgacgaggg cgagttcggt
    10201 gggttgcaga tatttttccg cccggctgga atgacagagc aatcccgtca ccgtggcatt
    10261 cgcgaccagc ttgtccgaca gggcctcgcg gatattcacg gcggcagcac tatcctggac
    10321 gatgacggga atcgaatcgg cttcgtcgtt cgtgccgatc tggccgacaa gattgtggtt
    10381 gttctcacgc caattgttga cgtcccagga aggagcccat tgctcgaaac cgttgaatag
    10441 aaaatgctga agcggctgca ggcgtttcct cgcctgatcg gatttccggt cccgcgatag
    10501 ccggtccttg cgaagcacca gggacggatc tttacgctgc caccaaccgt aggaaaacag
    10561 cgcactcgcg aagaaaacac cttcgatgac caggagatgg tcgaaatacg atcctcgttt
    10621 ctccgagacg agaagcttct tgagcgtgtg ggctgccagc gttcccttgc cggctttctg
    10681 cagcctcatg attccgcggt cgacggtcaa ataggccatc gaatttgtaa ctccggattt
    10741 cagaaacggc ccgacttgct gctctgcccg gcgaaacaat tcggcggccc cgaagcccaa
    10801 ggcaccaccg tcaacaacca acttaaccgc ttctaggtcc atcattggcg tctccctcgg
    10861 gaacacttcc catggcgcat ttcttgccaa aggcgcgtgt catttcatca ttctaaccgc
    10921 ccgccgcatg ttgttggcga gatgggtggc tccgccggtc gctatgagcg caacacccac
    10981 attcgagtct ggatctattc gcgcgttgcg ggccgttggt tccggtgtca cctcgcccgc
    11041 atcgcgatgg tgaaatgatc cgttcccccg agcccgtgtc gaaccttgat cggatcatcg
    11101 atgcttagga cgcgtttcag cacgtcgtgt tttttggcgg ggccggcgcg attgccgccc
    11161 actttgtaat tctcgaaatc gtaaatgcgg gccagccgct cgccttccgg cttgaaaatg
    11221 gacgcgtaaa ggacgatctc cgaaaatccc gcgcagcgct gccatagata tcgttggcaa
    11281 cattcggcga acgccacagc gagaccggtt tcggatttgc tgtattgcgg ggcgatgtag
    11341 atcgccccca cgataagttc gaggatcgcg tcatcgccga gcggtttcgg gatcggctcg
    11401 aaatggctcc cgtccatgcc cggctcctgc agcttgcctt ccagccagtc gagcagcgca
    11461 tcgggccgca ggtgaaagac atcgaagaac ccgatgttct taccgccgat atccgtcaac
    11521 ctgacgccca gcgctgctct cttgccgacg acgtcgcgta cgacctcggc ccgcatcgtg
    11581 tcgccgccga agacctggct tgcgaatttc gacaattccg ccaaatcgtc cccgtccgct
    11641 tgatatggcc ggcctatata ttccttgacc tcgccttcag gcacccagaa ctggctggcg
    11701 cgatagcgtt gccaaaaata gatactcatc cacacagttg cggatgccgc cgcgatggcg
    11761 ataaagacga taaaaggaag ttcggcgctg taacgcgcgg gaatcttatt gttcaacgca
    11821 agcgaaagcg ccgcgctgat gaatgcggcg gttccgctcc atgccgccca cgcggtgatg
    11881 acgctttttt tggtgatttt gaaaagatgc ttgttgtaag ccattgcgca aatccaatgt
    11941 cgcaaggcga tcataacggc gcgcgtgagg aaagttaacg ggcccggacg ccagatgtca
    12001 gcgtcttctc gaaaatcgcg cgcacctgcg attgggtttg cgcgaggcgg gtctcgagtg
    12061 ccgaaaaatc cggcgcgtcc ccggcacgcg caagcagcaa tttcaatccg cgtgtcgccg
    12121 tatcgggctt gaatggacca tcgacggcaa tccgcaggac ctgcgtcagg ccatgttcca
    12181 tgcgtgtcgc gtcaatcagc gcttcggcat cggctaaccc gaccagtccg tggccggcca
    12241 gtttctccaa ggccgcgatg gtgttctgat cgaggacccc cggctgggac ggcgccctgg
    12301 tcagttgcag gatttgcgcg atgaattcga tgtcgacgag gccgccagcc gcgaatttga
    12361 tatcccagat atcgcgggcc ggaaattgcg cggccaattt ctcgcgcatg gtgcgggcgt
    12421 ccgccaacag acgggtggaa tcggtcacgc catgcgtcag cgtcatgcgg atcgcgtcat
    12481 tgatacgcat ctgcaaatcc gcaggccccg acaacacacg cgcgcgcgtc agcgccatgc
    12541 gttcccaggt ccaggattgt tcacgatgat acgcgacgaa ggtctcgaac ctgaccgcaa
    12601 ccggcccttg attgcctgac gggcgaaggc gcatgtcgac ctcatagagc cgcccttcag
    12661 ccgtcatcgc ggtcagtccc gcgatgaagc gctgagcctg ccgcgcaaaa taggcggagg
    12721 tggcgagcgg cttgggaccg tccgatgaaa ccgcgtcgtc atcatgggtg tagatgaaga
    12781 cgaggtcgag gtcggaagcg gcggtcatct cgcgtccgcc gagcttgccc agcgccacca
    12841 cagcgaaggc gcctcctggg acgtgcccat gggcggcggc catttcacgc tcgacgatct
    12901 cctgcaagcc gcaaatcacc gtttccgcga ccgctgcata ggccgggccc gcctcttcgg
    12961 cggtgctgaa gccttggatg acctgcacac cgatgcggaa cgtctcctcc ttggtgaaac
    13021 gccgcgcggc atcgagagcg gcctcgtagc ccaacacacg gccgagatct tcggcgaaac
    13081 gcgcttgcaa ttcgtcgtgc gtcggtgtgc gtgtaagaaa gtccggatcg acgagcgcat
    13141 cgagcacggc cgcgtgacgg ccgagataat cggcaagctt tggcgcggag cctgcgatct
    13201 ccgcgatgag atcgagcaat tttggattgg caagcagcat cgagaacgcc tgcacgcccc
    13261 ccggcaagcc cgaaaggaag cggtcgaaat ggatgaacgc ggcatgcggg tcggcggtcc
    13321 tggccagtgc ctccagcaat gcgggaatca gctgcgtcag aagttcgcgc gcgcgcgcgc
    13381 cgcgcgtcgc acggatgcgc ccatggtgcc aactgcgaat gacggccgaa atatcggtgg
    13441 gccgctcaaa tcccatgcgg gcaagatttg ccaccgtttc cggatcttct tcgacgccgg
    13501 tgaaaaccag gctgccggtt tgcgtggtca gcggcgcttc gcgctcgaac agacgcgcat
    13561 aatggctttg aaccgtggtg agcttcgccc gcaatgcctc ttcgaaaatg ccggtgtcgt
    13621 cgaaacccgc aaagcgcgcc acccaatcta ttccgtcggg agatttgggc agggtgtgag
    13681 tctgctgatc ctcgatcatc tgcaaccggt gttcgatcct gcgtagaaaa cgataggcgt
    13741 cggacatgtc gtcctcggcg gcttgcgaga tgtggccgcg ctcacgcagt gcggcgagcg
    13801 cacccagtgt cgctggatcg cgcagggtcc gctcgcgccc gccgaggata agctgctggg
    13861 tctgtgcgaa gaactcgatc tcgcgaattc cgccacggcc gagcttgata ttgtgtcctg
    13921 ccaccgccac gcgcccgtgc cccgcatggc tgtgaatctg acgcttgatg gaatgaatgt
    13981 cctggatggc tgcgtaatcg agattcttgc gccagacgaa cggttccatg ttcttgagga
    14041 accgcgcgcc cgcgcttgca tccccggcgc attgacgcgc cttgatccat gccgcccgct
    14101 cccaattctg acccatgctc tcgtaataga gttccgccgc cacggtggag atcgcgatct
    14161 gcgtggcccc ggcatcgggg cgcagacgca gatcgacgcg gaaaacgtaa ccgtcggtgg
    14221 tcggttcagc gaggaccttc aggaagttct tcaccagatc gaccgccgcg gtgcgtttat
    14281 cgccaccacg cgcaaaggga aaacgttcgt cctcgtagaa aacgacaaga tcgatgtcgc
    14341 tcgaatagtt cagctcgaag gcgcccattt ttcccatcgc gagaacgaca agtcctgtgc
    14401 tgtcggccaa atctccgggc gggcgctctt cgaggccttg cattctcgcg gcacttgcga
    14461 ggaggaagcc gacgccgccc ttaacgcacg catccgcaaa ctgcgtcagc gtcccggtga
    14521 catcttccag atcgaacaat ccgccgatat cggcgagcgc aattgcaagc gcggcgcggc
    14581 gcttggcaat ccgcagccgg cgcattacgg acgtcatgcc ggcatcgtgc gcgacggcac
    14641 cggcctcggc attgagccga gcgagacaat tccgggggcc gtcctgcagg agctctggaa
    14701 gaaacgcctg ttcgcgcagc atcaagcgcg cgagataggg actgttgccc gcagcggctt
    14761 ccagaatcgc gcgaaaacca ggctcagcga gagcatccgg cgcggccccg ccaagatcgg
    14821 cgagcgtgcg tgccgcgcgc ccgggatcgg aaagcggcgg cagattcgaa ggcgcgttga
    14881 aacccgatgg catcattcgg gcctacacct tcacggcaca aacgcaaagc ccgcctggcg
    14941 cgaaaatcag tgaaccggct cgcgttggga ttcggcgcgc acaggcatta tcttcaccgg
    15001 acgcggctcc tccgcgaccc gcttgaacga cagcatcgcg cgcaggccgg gatgattgtc
    15061 ttcgaaggtg agttccgcct cgtgcatgcg cgcgaccgcc gcgacgaggc tgagaccaag
    15121 cccggtgccc ggcgaattgc gactcgattc caatcggaca aaacgttcgg tgatgcgcgg
    15181 acgatcctcg gttgggatgc ccggcccgtt gtcggcaaca accagtgccg gaccctgcgc
    15241 ctgattctcg agcgcgacca cgacagtgcc gccggccgga gtgtatttga tcgcattgtc
    15301 gacgagattg gccaaggctt gggaaatcag attgcgattg ccgtggacga gaccggcatc
    15361 ctgggctttg cggacgacga accgcaactg gttttcttcc gccagcggcc catagagctc
    15421 gctcacgcct tcgaggattt cggtgaggct gaaatcgcgc atcgtctcgc ggacactgcc
    15481 cgcttcggct tcggcgatca gaagaagcgc gttgaaggtg ccgatcagcc gatcggtttc
    15541 ctgcaccgca gcgtcaaaat cgcgcgccgt atcggaatcg ggcgcctgac gcatcgcagc
    15601 caattcaagc cggttgcgca ggcggttgag cggagagcga agatcgtggg ccacattgtc
    15661 ggagacctca cgcatcccgt gcagcagccg ttcgatccga tcgagcatgc gattgagatt
    15721 gcccgagaga tcgtcgaact catcgtccgc cggcgtaacc ggcacgcggc gcgagagatc
    15781 gccgcccatg atctggcggc tcgtccggtt tatgatatcc agccgggcaa ggaaattacg
    15841 gctgatgacc agaccaccgg cgagcccgag cacgaccatc aatcccgccg cccagtaaag
    15901 cgccgtcttg aacagattct ccagcacgcg tcgttcttcc acatcgcgcg cgacgagaag
    15961 atagaacccc tgcgtcagca taaagacctt gcccctcgcc cgcctcaatt ccgtggctgt
    16021 cccaatgcgc cgctcataag aaaactcgac gaagccgcgc gccgccgggc tgacttgcgg
    16081 ccagccgtcc aggctgccgg cgatcacgcg acgattgttg ttggcgagaa gatagatccc
    16141 ctgttcgcgc cgtatcgaac ggccgataat cgcatcggtc agtccgggca ggcccaggct
    16201 gtgatattgc tccaccagcc cggtgatctc agcctcgatg gtttcgtcgg tctcgcggtc
    16261 gagcacgaga gcggtgttcc aatagacgaa tcccaccagc gcgatcgccg aaacggcaaa
    16321 aatcgcaaga tagatggcga cgatccggaa tgcttggctg cgaaggatct taggcgtgcg
    16381 cacggatcat atatcccgcg ccacgaacgg tgtgcaggag cggcgcgtcg aagcccttgt
    16441 cgatcttggc gcgcaaacgc gaaatatgaa cgtcgatgac attggtctgg gggtcgaaat
    16501 gatactccca cacattctcc aacaacatcg tgcgggtgac aatctgtccg gcgtggcgca
    16561 tgagatattc gagaaggcgg aactcgcggg gttgcagctc gatgcccgag ccgttgcgct
    16621 tggcggtgcg cgacaacagg tcgagttcca gatcgcccac cgagaccttg gtcttcaccg
    16681 agcccggatc gcggcgccgg acgagcgcat ccaaccgtgc cagcaattcg gcaaaagcat
    16741 agggcttggt gaggtaatcg tcgcccccgg cgcgaagacc cttgatacgg tcatccacat
    16801 cggaaagcgc gctgaggaag agcaccggcg tcatgctccg ctcctcccgc atttcagcga
    16861 cgagctttag cccgtcaagc ttgggaagca tgcggtccac gattgcgaca tcatacggac
    16921 gggacagcgc cagggtgaga ccgatttcac cgtcaccggc atcatcgaca acgtggccgg
    16981 cctctttcag gcccttgacc agatagcgct gcgcttcaag atcgtcttcc accacaagaa
    17041 cccgcatgag caatattcct tctagtctgc tcagcccggt cagtagccga aaagacccga
    17101 acccagcggc ggcatgagga cacttccatg ccggttcaat aggagccgcc gccgggcatc
    17161 aacccggcgg cggtgagagg atcaagcctt agctaccttc aaggcgacgt aatgttgtcc
    17221 ctgatcgccg gcgaccagaa gcagcacggt ttcccgtccc gccttcttgg catcggcgac
    17281 accctgttcg atatcggcag ggttacgaac attcttattg ccgatgccaa cgatcacatc
    17341 gccggggcgg aagcccttat cggcggcctc gctgttcggg tccacggacg acacgacgac
    17401 gccattgacc ttcccatcga ccttgaactg ctcacgcgtg tccgcattca gcggcattag
    17461 ctccatgccg agcgatgtcg tgctcgcctg gcgattgttg ccgcggtctc cgcgattgga
    17521 gttcgccggc ttgtcggcgc tggcgacctg atcgggtcgc ggagcgatga tagccgtgac
    17581 gttgtgcttt tgtccgtcgc gcagcacgga gaaatccgcc ttttcaccgg cacggagacc
    17641 ggcaaccttg cgggtcagat cacgattgtc gtcgacatcc gtaccgttca aggaggtgat
    17701 gacgtcgccc tgcttgaagc ctgccttcat ggccggactg tcgtcgacaa cactggcgac
    17761 gatcgcaccc ttcgcattgc cggcaccgag gctggcggcc atgtcgggcg tcaagctctg
    17821 gatctggacc ccaagccaac cgcgtgaaac attgccatga tccttcaact gatcgacgat
    17881 cgtcttcacg atcgaggccg gaatggcaaa gccgatgccg accgaaccgc ccgaaggcga
    17941 gaagatcgcg gtgttcattc ccaccacgcg cccggtgaga tcgaaggtcg gtccacccga
    18001 attgccttga ttgatggggg cgtcgatttg gagataatcc gtatacggcc cattaccgat
    18061 gtcacggccg atggaggaga cgataccggc tgtcaccgtt ccgcccaagc cgaacggatt
    18121 gccgacagcc acgacccagt cgccgacgcg caggcgccgg tcgtcaccga gtgcgacgat
    18181 cggcaggtcc ttgacgccat cgatcttgat gagggcaacg tcggtatcct tgtcggctcc
    18241 aacgaggtgc gcttcatatt cacgcccgct cggaagcttg acggaaatct tcttgccttc
    18301 ctcgacaacg tgattgttgg tcacgatgta gcctgtggga tcgatgatga aaccggatcc
    18361 catggcttcc gaccgttgca tagggcggtt tcgcccctgg ccgccccggc cttgtccttg
    18421 tccctgacct tggccgtctc caccgaactg gcggaagaag tcacggaatg gggcaggcac
    18481 atcgtcaagt ccggcgacct gcggtccggc ggattcacga tccaccacca cggtcacgac
    18541 cgccgggctc acatgctcca ccagatcggc gaagctgaaa ggcgcgccct ggtcgagcag
    18601 acgcggcggc gtcggttgcg cctggcgcgc tgttacggcg gcgactgtgt gcagcccatc
    18661 ttcgggcaat tgcgtgtagg aatgggtttg ctggggcaca tagccgttcg cagtcccgct
    18721 atcatggctg aacacaaaac cgcccgcgcc aaaggatagt gcgacaatgg ttgcggcggc
    18781 cacgcccatg cgaagattgc gcttcgaggc aggtgaggat tttggattag gagtttcggt
    18841 catcatcgcg gcatttccca tgagggttga ttggggaccc cccttagctt gatgactaag
    18901 ttagggcctg attgttactt gtccatgttt caaaagttaa atatatgtaa ttctttcagg
    18961 ctagactggc acatcaagat cgtccggttc tgtaccgagg cggcggcggg cccgcaaaac
    19021 agtcacgcct atgaccgctc caccggccag caccaaaaga cctgggccga accaaagaaa
    19081 gaacgtgtcc tgccgtaccg gcggctgcat caggacgaat acgccatagc gggcaacgag
    19141 gaaatttttg atgtcgtcgt tggactgccc gctttgaatt tgctggcgga tgagccgccg
    19201 cagatcggcg gccaaggccg aattggactc atcgatggac tgaccctggc agaccagaca
    19261 gcgcaactcg cgctgcaaag ccttcgcgcg cgcttccagg accggatcgg ccaagggcgg
    19321 cacataggtg ctctctcccg gttgctcctc cgcaccgagg cccgcgacgg gcgccaaggc
    19381 ggcagcgctt agaaaagcca cgaggaacac cgcaagaact ctcaagacac agcctcactc
    19441 cgcgggttcg gaaacagccg ccgccgcgat acgccctgca cggaccttgg aggcaacccg
    19501 cgcacccaaa gcgatgaagc ccgcaagcgc catgatcacc gcgccaagcc agatgaatgg
    19561 tgccgccgga aagacatagg cgctcaccac ccagccgcca ttttggcggg catcgcccag
    19621 cacgacatac agatcggaga ccaccgttgt gcggatggaa gaattcgtcg tcaccgtttg
    19681 ctcgaccgga taactgcgct tttcgggatg gaccaccgtt acggtcttgc cgccggccat
    19741 gactgtgatt gcggcgcggt ctgcgaaata attcggccct tggacctttt ccgtgccgtc
    19801 aagccgaagc gtatagcctg cgatgttcat cgtttgcccg ggggccagaa cgcggatcga
    19861 ttcgcttcgc cacatggttg cgccaacgca gcccagcgcc accacaccca gcccggcatg
    19921 ggccaacgcc acgctccagg ccccggacgg gagcaaccgt acccgccgcc aagacccggc
    19981 acgacgcgcc agatcggtga tggagccagc gatcaaccac accccgagca tcaccgtgat
    20041 aagcccaagc gccggtgcgg gccgtgcgat cgccaacagt gccacgcccg cgaccaatgc
    20101 tgccgccatc gcgtaacgca aggcatacag cgtggtgcgc caattgcccc gccgccaaag
    20161 cgtgatcggc ccgagcggca tgacgagcgc cagaagtccc agcatgggaa ccaccgttgc
    20221 ggtgaaatag ggcggcccga cagagatttt gatgccttcc gcggcgtcga gcaccagcgg
    20281 gtacaacgtg cccgtcagga tcgtcgcggc agacgccgtc aagagaagat tgttcgtcat
    20341 gatgccggtc tcgcggctca ccagatcgaa cggcgcgccc ggcgaaagtt tcggcgcgcg
    20401 ccaggcgaag agtacgagtg gcgcggtgat cgccacgaac aggatcatca ggatgaaaag
    20461 tccgcgcctg ggatcgttgg cgaaggagtg caccgaattc agcacacccg aacgcaccag
    20521 gaaggtaccg atcagactga atgagaatgc ggcgatggcc agcagcaggg tccagctctt
    20581 gaatgcgccc gtgcgttcgg tcgccatcat cgaatggatc agggccgtgg cgatgagcca
    20641 gggcatcagc gatgcgttct ccaccggatc ccagaaccag aaccctcccc acccaagggt
    20701 gtagtatgcc caccaggatc ccgccgcgat ccccagcgtc agggccgtcc aggccgccgt
    20761 cgcgaaaggc cgcgcccggc gcacccaggc ggcatcgttg cgcttgtcga gaagggcggc
    20821 ggccgcgaag gagaacgccg ccgacagccc aacataaccg agataaagca gcggcggatg
    20881 gaaggcgagg ccggggtcct gaagcaaggg gttcaggcca ttgccctcga acggcggtgg
    20941 atagaaacgt tcgaaaggat cggaggtcag gagcacgaac acgagaaatg caaccgcgat
    21001 caaggcctgc acgccgagag cccgcgcacc caattccgca ttcgccggat tgtccgtcgg
    21061 ctgcgtcatg gcgatggcgg cggaataaat acccaggatc agcagccaca ggatcatgga
    21121 cccttcgtga ttgccccaga cgcccgagat tttatagatc agcggcttgg ccgtatgcga
    21181 attctccgcc accgccatga cggaaaaatc cgatgtcaca tacgcgtaca tcagcgtgaa
    21241 gaacgcgagc gcgataaaaa cgaagctgcc gagcgcagca ccccgtccca tcgccatcaa
    21301 ccgcacgctg ccccgcgatg ccccggtgaa accggagaat gcctgcacca gcgaaagcgc
    21361 cagagccagg cagagcgcaa gttgcccgag ttcgccggtc acgaggagcc tggcccgccc
    21421 tggccttctt gccaatgccc cgaacgtttc agcgcatcca ccacttcggg cggcatgtat
    21481 ttttcgtcgt gcttggccag cacctcgcgg gcgagaaaca cgccgctgtt gctgcgcgtt
    21541 cccatcgcca ccacgccctg gccttcgcgg aaaaggtccg gcagcacgcc ggaataatcc
    21601 accggcaccg catttttgcc gtccgtgacc acgaaatgaa cttccagacc ccgatcgcgt
    21661 ttgacgctgc ccttctcgac cagtccgccg atacggaagg cacggccctc agggactttt
    21721 tccggatggg cggcgatatc gctcgggctg tagaaaaaca ggatattgtc ctgaagcgcg
    21781 tagacggcca gcgccgccgc gcccccgccc gctagcagca gagtgatcgc aaaggcaagg
    21841 cgtctttgtt tcttgggggt catcttccgg tccgggccaa aacggcaaca atttatattg
    21901 tatcgctaaa acaggtccag cggcgagaaa aggcactttc ccgcgaggct ttttcgttag
    21961 ccttgctcca gcctgccgcc agtcctatca gtggcgggcg gttttcgcgg gcgcttacga
    22021 aatggtcctg cgcggctttg ttgcgttcgt cgtgctcgct gccctggcgt ttttcacgtt
    22081 tttctgggcg catgaggaac gcctgacctg gttcgatacc cgcccggtgc tggcggcggc
    22141 ggccaagtat gacgcgacaa tcattcgcga tcgcttcggc gtgccccata tcttcgggcg
    22201 ccgggatgcc gatgcgggct ttggcttggg ttatgcccat gcgacggacg atttccccac
    22261 gcttcagcgc gcctttcttt ccgcgcgcgg ccgcctggcc accttggatg gcgtcaaggc
    22321 ggcggagagc gattatcttg tgcaactcct gggcatctgg gatgcgatcg gggcgcatta
    22381 cgaaaccgac ctttctccgg agacacgcgc tctgctcgac ggctattcgg cgggcctgaa
    22441 tctctatgcg gcgcagcaca ggggccaggt gctcccgggt ttcacgccgg tccgcggcca
    22501 ggatgtcgtg gccctcttca tgctgcgatt gccgttttcc tacgggctgg aaaatcagct
    22561 ccacgccctg atcgccgggg gcgccggcaa ggtggcgccg gatgtctcgc agggacgcgc
    22621 aacggcgatt gccgtggcgc catcccgttc ggccgatggc gcgacacggc tgctggtcaa
    22681 cccgcagggg cccttcgaag gggtcctctc ctggtatgaa gcgcgcatcg tgtccggcga
    22741 aggctggaac ctcgcgggcg gtctgatccc cggctcgccc gttatcatgg cgggggccgg
    22801 acccggtttc ggctggggta tcgctcccaa ccatcctgat cttgccgacg tctatacgct
    22861 ggagaccaat cccaacgacc gctattttta tcgtttcgaa ggcgaatggc gccgccttca
    22921 gacgcgcgag gcgcgcatgg tggcgcggct ctggggaccc atccgcatca ccttccggcg
    22981 cgaaatcttg cgttcggttc aggggccggt gatccgcaac gcacgcgggc tattcgccgt
    23041 ccactatgcg ggccaggacg atcttaaagg ggtggaagcc ttcttccatc tcaacaaggc
    23101 gcagaatttc gaagatttca ccgccgtcct ggcgtcggga ggaattccga gtctcgattt
    23161 cgtctatgcc gacaaaactg gccgcatcgc ctctctctat aatgcagcgt ttccacagcg
    23221 cgccgacggc tatga
//
LOCUS       DASYEG010000208         5536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1514831, whole genome shotgun sequence.
ACCESSION   DASYEG010000208 DASYEG010000000
VERSION     DASYEG010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514831"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VGM72_09320"
     CDS             complement(<1..351)
                     /locus_tag="VGM72_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265506.1"
                     /translation="MEPLTFRNAWIVSVTCARRRVPLWAVLTIAGAALTCGLIAAPAL
                     YRLWAGLYRSEAPPLALSRILLGAGVFLPLYACALGMQFWEGRAARRASEFAAGEGLV
                     IGIGIGAFAFLAVIL"
     gene            425..1969
                     /locus_tag="VGM72_09325"
     CDS             425..1969
                     /locus_tag="VGM72_09325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4265507.1"
                     /translation="MPDWSSGYVTDVEYTEASYSAQAPQQLALTAIVNGFEPPDVSRG
                     FSYCELGCGKGLTSLILAAANPDAVFHAVDFNPAHIAHAQSRARAAQLSNIFWHEKSF
                     DELSGKAGDDLPMFDFMTMHGVWSWVAPEQQNAILKFMDRHLKPGGLVFVSYNGMPAW
                     NFMAPIQRILREFGGLSALRSDRSIDGAIAMLARLGEAKIIPPEFEKALKRLTEGPHR
                     RNLTYLAHEYLNDHWQPAYHLDVARAFASAKLAYVGTTDLLRNFTNLTLSDAQRTLLD
                     EIAVPELRDTLKDFCLDHWFHQDVYVRGARRMTEERRDSLLSSLCLALIRPVPTRIEI
                     AGPEETVWRPDAAAYGPFLDALQCRPHSVSELVCLPGVPASYKRKSLEVVGVLAGTGV
                     AAPYKEPDAATLASCDRLNRLAEAEGEVALTQNATIAAASLGLGMPMPAALFDVYLAL
                     RRGKKPDAVLFARHFVQRCKDDGGHPIVDGKSYEDEAAALTAVERDYQAKIEHIVPLW
                     QKLGMI"
     gene            2106..2858
                     /locus_tag="VGM72_09330"
     CDS             2106..2858
                     /locus_tag="VGM72_09330"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY4265508.1"
                     /translation="MRFLIAVSAGCCALVAANTGFSQPAPQLSPDEIVKALSCPPGTD
                     LNASGVCESVSAPASVPVAGAPGVEICAAPPDVPRVLVRTPDGDCVPAKEGTLGFDLG
                     AGVTGAASAGNATSHPPASAVSLTPRNNAPVAASVTLPRLDLLLTFDTDSASLTDQGT
                     ANAMAFAEALKRPELKQARFEIEGHTDATGTHEHNLALSERRAQAVKAFLTSHGVEAE
                     RLNAVGYASDRLAVPDQPASRANRRVVARRLQ"
     gene            3028..4620
                     /locus_tag="VGM72_09335"
     CDS             3028..4620
                     /locus_tag="VGM72_09335"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEY4265509.1"
                     /translation="MRTAQPPAVARSGDILAAPEPGGCPLENSNVRLTLKAVEFRDAP
                     DLPPDTLAAAYQGLIGREIPVSAICQIRDRATTILFRRGLLARVEIPEQTIMQGRLSL
                     DVIAAYVAAIQVQGDAGPAQKKVEDYIERLRGLRPFDINAAQRYLLLASDIPGVVISA
                     SLRSSSQGRGAVELDIQVGHVPFGAVLASNNFGAKTAGRMGALMRADFNSLTPFGERT
                     SLIAYSTFDGNWREQQAIQGVEEARIGSDGLVAKLSFAYASTHPGDILGPLNITGKSI
                     VGDFRLAYPIIRSRSLNVSATAGATYVRQDIDVSTIPFTRDRLSLFIARLSADKAWSD
                     VPVTLGGGIELHKGVGSALAGSSACSPASPCLLSRFEARPDAFVQRADGRIAVQWLPL
                     VSTVATFQAQHSDVPLLAYEEFAIGNLTVGRGYDPSVLSGDSGIGGAAELQIGPFNPG
                     GIAFFAPGLTFIGFFDIARVWNKDTGGVVRSLRSTGAGINLQIVQHVQIQLLYSKPLD
                     NVSGLLASKPSSRFLFNMTVGF"
     gene            4637..>5536
                     /locus_tag="VGM72_09340"
     CDS             4637..>5536
                     /locus_tag="VGM72_09340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HEY4265510.1"
                     /translation="MKPLPNSASGLKARLLCCTAMSLFAAPALADTVTTVGTIANWKD
                     NAGNNTATPGGSLLSDDGTTVAVSLAGSRTILDWKSSDPLNIASGKALSFQFLGRSDI
                     VLNRITAGSATISGTLTGTVAGTPGGNIWIAARDGILFGDGAVVSTGGLLATTSPMTT
                     TDANFLAGTEAVSGANHIFTFGDAVNAVSIGDVHITVNGGLLAFVAPVVTSAANTAGN
                     GVDGTGSVLYGAANAFKITLAPDSATNWDLVSFEVPAGKGSSAAGSIIGLAGQTNITG
                     SVYLAAVNSTVPTRIISVSGPIAT"
BASE COUNT         1031 a         1745 c         1677 g         1083 t
ORIGIN      
        1 gagtattacg gcgagaaatg cgaacgcccc gatgccgatg ccgatgacca gcccttcccc
       61 ggcggcaaat tcgctggcgc ggcgggccgc ccgtccctcc caaaattgca tgccgagggc
      121 gcacgcatag agcggcaaga acacgcccgc cccgagcaat attcgtgaca gcgcaagcgg
      181 gggagcctcc gaacgataga gcccggccca cagccgatac agcgccggcg cggcgatcag
      241 gccgcatgtc aatgctgcgc cggcaatcgt gaggactgcc cataggggga cgcgccgccg
      301 cgcgcatgtc acggaaacga tccacgcgtt gcggaacgtc aaaggctcca tgtcactgcc
      361 ggattgcccc agggactcta taatgcatta gatcgggcgt tttcaaaacg ggtcaggggt
      421 gtgcatgccg gactggagca gtggatatgt tacggatgtc gaatatacgg aggcctccta
      481 ctcggcccaa gcgccgcagc agcttgcgct caccgccatt gtaaacggtt tcgaaccgcc
      541 cgatgtcagc cgtggattca gctattgcga actcggatgc ggcaaagggc tgacgagcct
      601 catcctcgcc gccgcgaacc ccgatgccgt tttccacgcc gtcgatttca atccggcgca
      661 tatcgcccat gcccaatcgc gcgcccgcgc ggcacagcta tcgaacatct tctggcacga
      721 aaagagcttt gacgaactct ccggcaaggc cggcgacgat ctgccgatgt tcgatttcat
      781 gaccatgcat ggggtgtgga gctgggtcgc acctgagcag caaaacgcca tcctcaaatt
      841 catggaccgg catctgaaac cgggcggcct ggttttcgta agctacaatg ggatgccggc
      901 ctggaatttc atggccccca tccaacggat attgcgcgaa tttggggggc tttccgcgtt
      961 gcggagcgac cgttcgatag acggcgcaat cgccatgctg gcgcggctgg gcgaggcaaa
     1021 aatcatcccg cctgagttcg aaaaggccct gaaacggctc accgaggggc cgcatcgccg
     1081 caaccttact tatctggcac atgaatatct caacgatcat tggcagcctg cctatcacct
     1141 cgacgtcgcc cgcgcctttg catcggcaaa actggcctat gtcggcacca ccgacctgct
     1201 ccgaaatttc accaatctca cgctttcgga cgcgcagcgg accctgctcg atgaaatcgc
     1261 cgtgccggaa ttgcgcgaca cactcaagga tttctgcctc gaccattggt ttcaccagga
     1321 tgtctatgtg cgcggcgcaa ggcgcatgac cgaagagcgg cgcgactcat tgctctcctc
     1381 gctctgtttg gcgctgatcc gccctgtccc aacccgcatc gaaatcgccg gaccggaaga
     1441 aacggtctgg cggccggatg cggcggctta tggaccgttc ctggacgcgc tgcagtgccg
     1501 cccacacagc gtttcggagc tggtctgcct tcctggcgtc cctgcaagct ataagcgcaa
     1561 aagtcttgag gtcgtggggg tgctggccgg tacgggggtg gcggcgccat acaaggagcc
     1621 ggacgccgcc acactggcga gctgtgacag gctgaatcgc ctcgccgagg ccgaagggga
     1681 agtcgcgcta acgcaaaacg ccacgatcgc ggcggcttcg ctcggcttgg gtatgccgat
     1741 gccggccgcc ctattcgatg tttatcttgc gctccgccgc ggcaagaaac ccgatgctgt
     1801 cctatttgcg cggcattttg tccaaagatg caaggatgac ggcggccatc ccatcgtcga
     1861 cggcaaatcc tatgaagacg aggccgcggc gttaaccgca gttgagcgag attaccaggc
     1921 gaagatcgag cacatcgtac cgctttggca gaaactcgga atgatctgaa tgcagaccgc
     1981 cggcaggccg acggcggaaa tattacccat gaaagcgggc tgcgttaagc ttgcggccaa
     2041 agcgtatctt gccgtatcag cctcaaacgg aatatagaaa acctgccatt tttgggaaaa
     2101 cgaagatgcg gttcctgatt gcggtttccg ccgggtgctg tgcgctggtt gccgcaaaca
     2161 cgggtttctc ccaaccagca ccccagctct caccggacga aattgtcaaa gcactgagct
     2221 gtccgccggg caccgatctc aacgcaagcg gcgtgtgcga atccgtttcg gcgccagcat
     2281 ccgttcccgt ggccggcgcg cctggggtgg aaatctgcgc cgcgccgccg gatgtgccgc
     2341 gcgttctcgt caggaccccc gatggggatt gcgtcccggc caaagaaggc acactcggct
     2401 tcgacttggg cgcgggggtc acgggggccg catcggccgg caatgccact tcccatcccc
     2461 cggccagtgc ggtctcgctc acgcctcgca acaatgcgcc ggtcgccgct tcggtcacgc
     2521 tgccacgtct cgatctgctc ctgacgttcg atacggattc ggccagcctc accgatcaag
     2581 gcaccgccaa cgcaatggcc ttcgccgaag cgctgaagcg gccggaactg aagcaggcgc
     2641 gcttcgagat tgaaggtcac accgatgcga ccggcaccca tgagcacaat cttgcgctct
     2701 cggagcgccg cgcgcaggcc gtgaaggcct ttctcacgtc gcatggggtg gaggcggaac
     2761 gcctcaacgc cgttgggtat gcttccgatc gtttggccgt acccgatcag ccggcatctc
     2821 gtgccaatcg ccgcgttgtc gcgcgcaggc tgcaatagcg caaatgcttg tcggtgcgat
     2881 gggcgcgttg gtcacgcctg gggggccgtt ttgtttggga tgttttcgcg ccggttggcg
     2941 ttagcgctgg gaattcttgc gcccgccgtc tgggcgccat ctgcctttgc ccaaaacatt
     3001 cttcctcctc aaattctcga tcccgcggtg cggactgcgc aaccgcctgc cgtggcgcgt
     3061 agcggcgata ttctggccgc gcccgagccc ggtggctgtc cgctcgaaaa ttccaacgtt
     3121 cggttgaccc tcaaggcggt ggaattccgc gatgcaccgg acttgccgcc ggatacgctt
     3181 gctgccgcct atcagggact gatcggacgg gaaattccgg tcagcgcgat ctgccagatc
     3241 cgcgaccgcg caacgacgat cctgttccgg cgcggtctgc tggcacgcgt cgaaatcccg
     3301 gaacagacca tcatgcaagg gcggctcagc ctggacgtca tcgcggccta tgtagcggcg
     3361 atccaagtgc agggcgatgc cgggccggcg cagaaaaaag tcgaggatta tatcgaaagg
     3421 ctccgcggcc tgcggccgtt cgatatcaac gcggcccagc gttatctgct gcttgcttcc
     3481 gacattcccg gtgtcgtcat ttccgccagc ctgcggtcct cctcgcaagg ccgcggcgct
     3541 gtcgaactcg acatccaggt cggtcacgtc ccgttcggcg cggtcctggc cagcaacaat
     3601 ttcggcgcta agaccgcggg ccgcatgggt gcgcttatgc gggccgattt caacagtctc
     3661 accccatttg gcgagcgcac ctcgctgatc gcctattcga cgttcgatgg gaattggcgc
     3721 gaacagcagg cgattcaagg cgtcgaagaa gcgcggatcg gaagcgacgg actggtggcg
     3781 aaactctcgt tcgcttacgc cagcacgcat ccgggcgata tcctggggcc cttgaacatc
     3841 acgggcaaat cgattgtggg cgattttcgt ctcgcctatc cgatcatccg ctcacgctcg
     3901 ctgaatgtga gcgcgaccgc gggcgcgacc tatgtccgcc aggacatcga tgtgtccacc
     3961 attccgttta cgcgcgacag gctgagcctg tttatcgcgc gactttccgc cgacaaggcg
     4021 tggagcgatg tcccggtgac tctcggcggt ggtatagaac tgcacaaggg cgtcggatcg
     4081 gcgcttgcgg gcagcagtgc ctgttctccg gcatcgccct gcctgctgtc gcgcttcgaa
     4141 gcgcgccccg atgcgttcgt tcagcgcgcc gatggccgga ttgccgttca atggctgccc
     4201 ttggtttcga cggtcgccac gttccaggca cagcacagcg atgtgccgct gttggcgtac
     4261 gaagaatttg ccattggcaa tctgaccgtc ggacgcggtt acgatccttc cgtcctttcg
     4321 ggggacagcg ggatcggtgg agcggccgaa cttcaaatcg gcccgttcaa tccgggcggc
     4381 atcgcgttct ttgcgccagg cctgaccttc atcggctttt tcgacatcgc tcgggtatgg
     4441 aacaaggaca ccggcggcgt cgttcgcagc ctgcgttcca ccggtgccgg catcaatctg
     4501 cagatcgtgc agcacgttca aatccagctt ctctattcca aaccgctcga taatgtttcg
     4561 ggcttgctgg cttcgaagcc ttccagccgt ttccttttca acatgaccgt gggattttga
     4621 gtgtgaaagc gcgaccatga aacctctgcc caattccgct tcggggctca aggcgcgtct
     4681 gctttgttgc accgcgatgt ccttgttcgc cgcgcctgcc cttgccgata cggtcaccac
     4741 agtcggcacg attgcgaact ggaaggacaa tgcggggaat aacaccgcga caccgggcgg
     4801 aagtttgctc tccgatgacg gcacgacggt ggctgttagc ttggcgggat cgcgcaccat
     4861 cctggattgg aaatcaagcg atccgctgaa catcgccagc ggcaaggcgc tgtcgttcca
     4921 attcctcggg cgctccgaca tcgtattgaa tcggattaca gccggctccg ccaccatcag
     4981 cggcacgctg acaggcacgg ttgccggaac tcccggcggc aatatctgga tcgcggcgcg
     5041 tgacgggatt ctgttcgggg acggcgccgt tgtcagcacc ggcggactgc tcgccacgac
     5101 atccccgatg accacgaccg atgcaaattt cctcgcgggc acggaggccg tgtccggtgc
     5161 caatcatatt ttcaccttcg gcgatgccgt gaacgcggtt tcgattggcg atgtccacat
     5221 cacggtcaat ggcggcctgc ttgccttcgt ggcgccggtg gtgacgtcgg ctgcgaacac
     5281 cgcgggaaac ggcgtggatg gcaccggttc ggtgttatat ggcgcggcca atgcattcaa
     5341 aatcacgttg gcgcccgaca gtgccacgaa ttgggatctt gtcagtttcg aggtgcctgc
     5401 cggaaaggga agttcggcgg cgggaagcat catcggtttg gccggacaga caaacatcac
     5461 cggcagcgtc tatctcgccg cggtcaattc gacggtcccg acacgaatca tatccgtcag
     5521 cggtcccatc gcaacc
//
LOCUS       DASYEG010000209        15772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4560318, whole genome shotgun sequence.
ACCESSION   DASYEG010000209 DASYEG010000000
VERSION     DASYEG010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 15772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15772
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4560318"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="VGM72_09345"
     CDS             complement(<1..1213)
                     /locus_tag="VGM72_09345"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4265511.1"
                     /translation="MSTVPSASGGAVAPPRSFAALRHPGYRAYLSGNALAMMADSIEH
                     VITYWVAFQKFHSPALGGFAVLSHWLPFLLFSTFMGALADRVDPRRLVQIGMALFMFC
                     SLAWSVLILTGSLQMWHAALLLLIHGCAGVFWGPPGQLLIHDIVGPTQLPSAVRLMAT
                     SRYLGLLAGPAVGAVFLLAFGPAHGLMLNVVLYLPLMLWLWKAPYGPRFRPARATPAA
                     AVRGLADILATARAIAGERTIVSMIMLSGCASLIVANAYQAQMPEFGRDLGHGDAGVM
                     YSALLGADAAGALTGGIVLESRGLPPNPRTAFVLAMLWCCAIAGFAVTNFYPLALVVL
                     FAAGFLELSFNSMAQALVQMHAPPAIRGRVIGLYSMAGLGLRAFSGITVGIMGSVIGI
                     HWSLSLSALTLL"
     gene            complement(1402..2196)
                     /locus_tag="VGM72_09350"
     CDS             complement(1402..2196)
                     /locus_tag="VGM72_09350"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY4265512.1"
                     /translation="MASALKLVKNGIVVSLAHAEPQTVEVDVNAAGVFHRVTNQITDG
                     GTTDNYQVSYHGQTIAHIDSWCHFFENGQMYNGVPVKDNVTNESGCKKGGIMNWKNGI
                     TTRAVLYDIAALKGVDSVDPNVPITRADLEAWEKKSGVKIGPGDIPLLYTGRWKRRAA
                     LGPWTGQVAGYYPDTIPWMHERLPAFIGHDFNIDWNPRPGWEGMRNPIHVAVLNWMGI
                     NIVECLDLEELVKTARRLNRYEFVISFAPLPVEGGTGSPVNPVAIF"
     gene            2640..3548
                     /locus_tag="VGM72_09355"
     CDS             2640..3548
                     /locus_tag="VGM72_09355"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY4265513.1"
                     /translation="MALDVADQTIAEPGRAVRHTRSGKELIYRHSLLVRLAHWINVLC
                     VVLLLMSGLQIFNAHAMLHWGIKGDEADPAILSIYAEEQLEGPPIGWVQIGTAKFNTT
                     GWLGVFRDRDGFLSGRAFPWWLTIPSYQNLAEGRRWHFFFAWLFALNGLAYLVYGFVA
                     RHFQRDLAPTRFDFAHIGRSILDHLTFKHPRGEAAKRYNVLQKMTYIAVIFVLLPLMI
                     ATGLTMSPGVDSTAHWLLDVFGGRQSARTIHFFAATLIVLFILVHLIEVLISGVFNEL
                     RSMITGWFAVKPEKPGAKSPEERAKP"
     gene            3545..4324
                     /locus_tag="VGM72_09360"
     CDS             3545..4324
                     /locus_tag="VGM72_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEY4265514.1"
                     /translation="MTRELITRRAALTGVAGTAGLILSGCDRLSQNQTFQRMLEAAEK
                     LNLGGQRLVLNSSAPLAREYRKSDISRVFKANGTVRPDGEEYRRMAFEEFQNWKLQIG
                     GLVNKPLSFSLEELRAMPSRTQITRHDCVEGWSAIGEWTGVPLSALLNQAGLKPDARY
                     IVFRCADDLIGSRDGSGLYYESIDLVDAFHPQTIMAYAMNGNILPIEHGAPLRLRVER
                     QLGYKMAKYVMRIEAVEDFSKIGIGKGGFWEDRGYEWYAGI"
     gene            complement(4336..5307)
                     /locus_tag="VGM72_09365"
     CDS             complement(4336..5307)
                     /locus_tag="VGM72_09365"
                     /inference="COORDINATES: protein motif:HMM:NF017773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4265515.1"
                     /translation="MTTTVYFATNRVISGAASDYRNYGDSIVSPTDPNQITYATAFVE
                     DANLTADTTGAIKSIQSISKGRFSPEALADLSTPGRNLLIFIHGFDNNFENAITRAAF
                     NQQWFAQSGAPGANTTVISFSWPSLGKLVSFPILWSDYIHDQTVAGQSGPHIMSFFAN
                     LEPIVTAVRKSGRRSFLLAHSMGNWALQAAVESWFLHGNGAADLFDEAFLCAADERYD
                     SFSFPTPGRLCDLKQLTKRTSVYYSTADAVLSLSMAVNLGAKRLGQEGPHDRVDQNLF
                     PPAKFRMVDCSSFNDYDRDPGSSHQYYRRSPKARADIAAVMGGQKLV"
     gene            5524..5982
                     /locus_tag="VGM72_09370"
     CDS             5524..5982
                     /locus_tag="VGM72_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY4265516.1"
                     /translation="MAVTLKVNGKTHALDIEPDMPLLWALRDEIGLTGTKFGCGIAQC
                     GACTVHIDGQAMRSCSVPVSTAAGKDITTIEGLAAADGKLHPVQAAWIAEDVPQCGYC
                     QSGQIMAAVALLKTKPNPTDEDINGITNICRCGTYIRIRRAIHRAATTKA"
     gene            5998..8199
                     /locus_tag="VGM72_09375"
     CDS             5998..8199
                     /locus_tag="VGM72_09375"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY4265517.1"
                     /translation="MGHVEPIARDEALSRRKFIVTGMSAGGGLALGFAFPGLASARPL
                     GEAPWSNDALAVSNEVNAWIVIDPDETITIRIPHSEMGQGAGTSNPMLVAEELECDWA
                     KIKGEFASPNRNVRENNVYKDQNTVGSRGVATSWQYLQQAGASARVRLVQAAAHQWNV
                     PASECVAQNGAVIHTLTKRTLTYGKLAGDAAKITLDKEPAIKTPDQYKLVGKPLARLD
                     TPVKVDGRAKYGIDTKVPDMVYAAIISCPVVGGTVASVDESGLAGKRGIVKVVKLKDA
                     VAVVADNTWRAMRGLDALKIDWNYGPAGTSDSAQMRSMYRATLDEPMIQARHDGDAPG
                     ALANAGKIIDSVYEVPHLAHTPMEPLNATAHFQADRVDVWIGTQTALGTLQQGAAASG
                     LKPEQVYVHNAYLGGGFGRRSKNDEMEQAIAISKEVGKPVKLTWSREQDVRGDRYRPQ
                     AAARFKAALGPDGKPSVIDARVAVGSINRSLGRPVDGSIEGQAVDGIVNSAYKIPNFY
                     VGGVMKNTHLPVSFWRSVGGSQNCFFYESFIDELAHAAGKDPYQFRRDMVDRVDFVGV
                     LEKLAEKSDWKQALPKGRGRGIAVAENHGSVVGTVAEVTVDAQDRIRVDRMVVAVDCY
                     HVVNPSIIEAQMQSGAIFGMTAAFYGEMTVKDGQVQELNFDTYRMVRLAEAPKIEVYL
                     VPSGGPKFGGIGECGTATIAPAITNAVFAATGKRVRQLPLKNIKLSQLASL"
     gene            8334..10049
                     /locus_tag="VGM72_09380"
     CDS             8334..10049
                     /locus_tag="VGM72_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4265518.1"
                     /translation="MDDFFAALRDRHVYRVAAAYVVVAWILLQLYNNLQPILKLADWV
                     GTLVLVVLVTGFPVSLLLAWVYQITPNSGAWLSQRSAKTPPLFQPKSTGGISIAVLPL
                     ANLSGDSTQEFFSDGMTDEITSALAKIRDLRVVGRSSAFQFKGQYKDLRAIGRALSAR
                     YLIEGSVRKAGDRVRITAQLAEAESGSQRWSETYDRELKDVFAVQEDIAHAIAAALHV
                     SLGLKRGESLVPSRTSHLDSYQDYLRAKALVRARGPRESGGPLTEAAKLLEQAVARAP
                     NYAPAWAVLAQAYSLIPAFSPPPTGGSIAEYRSRIMESLTKAETSAQRAIRLDPSHAE
                     GYIGLATVHTFRRQFIPAEDLFQKALSLDPNNPDALQIYSVALATAGRIKDALPLRLR
                     LRALEPFVPVFNAFTAQILWLNGQDDAAIAILKALPRNTAFAAISLAQIHAAAGRYGE
                     AADALLEAAPGFYRPGAVEIAAGLLRTAPVHAVSVESLPKLGPLSFVYLYAGAPERAL
                     EPAEDGDPDAPTAMLLWHPSAAAARKTVRFKTLVHGTGMVEFWRARGWPDLCRAQGPD
                     DFVCD"
     gene            10162..11190
                     /locus_tag="VGM72_09385"
     CDS             10162..11190
                     /locus_tag="VGM72_09385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEY4265519.1"
                     /translation="MNGIGPRDRVRLAVRIAIPAILTAALSACVTKPVPRMPAPVVNH
                     APVAAASPAPPPPAMTAPNPIYKVGNPYQVAGIWYYPKEQPDYDETGIASWYGADFHG
                     MLTANGEVFDRNAISGAHPTLPMPVNVRVTNLQNGRSLVVRVNDRGPFINGRVIDLSE
                     HAADLLGFRTTGTARVRVTYLGRADLFGPGLAPPSQETPPEIAMAVPAVPVTGVDATP
                     LPPVMGAKVEPPPPMVSLPKPVQHVEIAQLDAVADGHVTEMAVPKATAIYVQAGAFTS
                     PTNAGAVAAKLQSTGARVYPGRKDGRPIYRVRIGPFQDVGAADSALVQVQTLGHSDAQ
                     IVVDSVSS"
     gene            11262..12428
                     /locus_tag="VGM72_09390"
     CDS             11262..12428
                     /locus_tag="VGM72_09390"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEY4265520.1"
                     /translation="MFFSDHVRPLAFGLAACASVTAPQAVQAEILTTAKQAIILDYDS
                     GETLYCKSCEEPMPPSSMSKLMTVELVFQRLKDGRLKPEDTFHVSETAWRQGQKDNES
                     KMWVALNSDVAVDDLLKGIIVQSGGDACVVVAEALGGSESGFADMLNARAKELGLAHS
                     HFVNSSGLPDPGQFMSAEDIAKLSAHIVRDYPDYYKYFAIPDFTWSKIKQANRNTLVD
                     ENIGVDGLKTGHTEVGGYGIVASSLRNGRRLVLVINGMKSERERINEARRLLDIGYRE
                     FKDYKLADKGAVLGDAQIWGGAKNAVGVTVKDPLVVMLPVDSRRDMKVTLRYDGPVRA
                     PVVAGQQIGTLTVSVPGKPDKVVPAVAIESMPSNSFLDKMMIGLQTLIFGADKA"
     gene            12444..13073
                     /gene="tmk"
                     /locus_tag="VGM72_09395"
     CDS             12444..13073
                     /gene="tmk"
                     /locus_tag="VGM72_09395"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY4265521.1"
                     /translation="MVPGKFISLEGGEGAGKSTQAKLLRSALEARGHSVLLTREPGGS
                     PGAEEIRKLLVEGEPERWTPLGETLLFLAARADHVTRAIAPALASGAWVISDRFADST
                     VVYQGIARGLGMARVRALQMAALGDFMPDLTLVLDVNPQDGLARAGARGAAENRFERF
                     DDAFHRRLRDGFLAIAAEAPQRCAILDAAGTVDAVAAKIWNIVAERLRP"
     gene            13070..14119
                     /locus_tag="VGM72_09400"
     CDS             13070..14119
                     /locus_tag="VGM72_09400"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEY4265522.1"
                     /translation="MSPARRETEIEESDRIEGQAHPRETYALVGQDAALSRVARAIRS
                     ARPPQAWLIAGPPGIGKATLAYRIARYLLRYGAGAAGPADLCVPANDPVSRQIEAGAH
                     PGLLVLKRQLNERGKLRTVLDVAEIRRLGGFFGMTAEAGGWRVAIVDSADEMNDNAAN
                     ALLKILEEPPGRGLLLLISHAPGRLLPTIRSRTQRLDLKPLSDSVLDEALVRLLPDGS
                     SDDRAVLVRLAEGSLGLALRLSNGDGLKLASDAQDMIAVRGAPDFPAVLAMAERVSRA
                     ADGLQHFGAFLSQAISSRIRERSRRAEGDDRAVEMWERLNALYERATGLHLEPRQTIL
                     ASAREIAGAKRRGAL"
     gene            14129..14908
                     /locus_tag="VGM72_09405"
     CDS             14129..14908
                     /locus_tag="VGM72_09405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY4265523.1"
                     /translation="MLVDSHCHLDFPDFGAELDSVLARARAAGVGHFLTIGTELKRFP
                     GVLAAAETAADIHCTVGVHPHEAAEEPLCGPDVLLKESRHAKVVGFGESGLDYYYNHS
                     PREAQIANFRAHIAAAREAKLPLVVHTRDAEDDTIAILREERERGAFTGVIHCFTGTA
                     RLAGAALELGFYISVSGIATFKKSDVLRATLKEVPLDRLLVETDAPYLAPQPARGKRN
                     EPAFMVHTAAMLAELKDVAHADIARATTENFFRLFTKAGPA"
     gene            14905..15723
                     /locus_tag="VGM72_09410"
     CDS             14905..15723
                     /locus_tag="VGM72_09410"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4265524.1"
                     /translation="MIAGDRLCATILGCGSSAGVPRLGGPNGVGDWGACDPADPKNRR
                     RRCSLLVERGRTAVLVDTSPDMREQLLAARVIHLDAVLMTHPHADQTNGIDDLRPLTF
                     LMKRRVDMYADEAALAHLSRQFSYCFETPAGTEYPPIITGHVIAEPFSPFHVNGEGGA
                     IPVVAFWQGHGPVRSLGFRSGGLAYSSDVSDLDAAAFAALEGVETWILDALRYTPHPT
                     HANLETALAWIARVKPRRAILTNLHIDMDYAELAAQLPAGVEPAYDGMIVEVPL"
BASE COUNT         2950 a         4922 c         4959 g         2941 t
ORIGIN      
        1 cgagcaacgt cagcgcgctc aacgacaacg accagtggat gccgatcaca ctccccataa
       61 tgccgaccgt gatgccgctg aaggcgcgca aacccagtcc cgccatgctg tagagcccaa
      121 tcacacgacc tcggatcgcg ggcggcgcat gcatctgaac gagcgcctgc gccatcgaat
      181 tgaacgacag ttccaagaac ccggccgcaa acaggaccac gagcgccagc ggatagaaat
      241 tcgtcaccgc aaaccccgcg atggcacagc accacagcat ggccaggacg aatgccgtgc
      301 gcggattggg cggcagtccg cggctttcca gcacgatccc gccggtcaat gcccccgcgg
      361 catcggcccc cagcagcgcg ctgtacatga cacccgcatc gccatgaccg agatcgcggc
      421 cgaattccgg catctgtgcc tgataggcat tggcaacaat cagcgaggcg cagcccgaca
      481 gcataatcat ggatacgatg gtgcgttcgc ccgcgatggc gcgcgcggtc gcaaggatgt
      541 cggcgagccc gcggaccgcg gcagccggtg tcgcacgggc ggggcgaaac cgcggaccgt
      601 agggcgcctt ccacagccag agcatcagcg ggagatagag cacgacgttc agcatcaacc
      661 cgtgcgccgg accgaacgcg agcaggaaga cagccccaac cgccggaccg gcaagcaagc
      721 ccaggtaacg tgacgtggcc atcaggcgca ccgcgctcgg aagctgcgtg gggccgacga
      781 tatcgtggat cagcaattga cccggcggac cccagaacac gccagcgcat ccatggatca
      841 agagcagcag ggccgcatgc cacatctgca ggctgccggt caggatcagc accgaccagg
      901 cgagcgagca gaacatgaaa agcgccatgc cgatttgcac cagccggcgc gggtccacac
      961 gatccgcgag cgcgcccatg aatgtcgaga acagcagaaa gggcagccaa tgcgacagca
     1021 ccgcaaaacc acccagcgcc ggcgaatgga atttctggaa cgccacccaa taggtgatca
     1081 catgctcgat gctgtcggcc atcatcgcca gcgcattgcc ggagagatag gcgcgatagc
     1141 cgggatgacg cagagccgcg aaggatcggg gcggcgctac ggcacctccg gaagcggaag
     1201 gaacggtgct caaatgggtt tcctacgtcg gacgggcagt ccccgtctgg caatggcctg
     1261 tggctcaacg catatcggcg cttggagcca cggtcaatga gatgcgtggg cggcgcaaag
     1321 cctggacggc gcagcccgga ccatccctcg gcattcccgc cttgtgccgt tcggagacgc
     1381 tctatgactt caggacttcc cctagaaaat ggccaccgga ttgaccggcg agcctgtccc
     1441 gccttccacc ggcaacggcg cgaatgaaat cacgaactcg tagcgattta accgccgcgc
     1501 cgttttgacc agctcctcga gatcgaggca ctccacgatg ttgatcccca tccagttcaa
     1561 gacagccaca tggatcggat tgcgcatgcc ctcccagccg ggccgcggat tccaatcgat
     1621 gttgaagtcg tggccaatga aagccggcag cctttcatgc atccacggaa tcgtatccgg
     1681 ataataaccc gccacctgtc ccgtccacgg acccagcgcc gcacgccgct tccaacggcc
     1741 ggtgtagagc agcgggatgt cgccgggacc gatcttcaca ccggatttct tttcccatgc
     1801 ttcgaggtcc gcgcgcgtga tcggcacatt ggggtctacc gagtcgacgc ccttcagcgc
     1861 tgcgatgtcg taaagaacgg cgcgcgtcgt gatgccgttc ttccagttca tgatgccgcc
     1921 ctttttgcag ccgctctcgt tggtcacatt gtccttcacg ggcacgccat tatacatctg
     1981 gccgttttcg aagaagtgac accagctgtc gatgtgcgcg atggtttggc cgtggtagct
     2041 cacctggtaa ttgtccgtcg tgccgccatc ggtgatctgg ttggtgacgc ggtgaaaaac
     2101 accggcggcg ttgacgtcga cctcgaccgt ttgcggttcg gcgtgggcga gcgacaccac
     2161 gattccgttt ttcaccagct tcaacgcgct ggccaccttc tcgggcgtga tgagctggtc
     2221 gtgccctggg actcctgtcc caacggagca aacttgcccc aggtcttgaa ctccgtccgc
     2281 catttctcat attgggcccg cagcgccgcc gtgtccggcg gagcgaccgg ctgcgttgcc
     2341 tgcgcgggag cagcgtgggc ctgcgttcgc aacgcaaatc caccggtcag accagccacg
     2401 gccaggccgg cacttattgc gaccgatgtc agatgatttc gcatgcgcgt ctcccctgtg
     2461 ttttggccca gtcctgtgtt ttggcccggt gtttgaattc ccaaaccgga ttgcccattt
     2521 ggccggtcaa gcgtggccgc atgatacacg cgtgaggcgc gctgaggata gcagcgcgat
     2581 tcatccccaa ggccatgcga ctgtgctaga aattgtccgt gcactcatta ggatgaccca
     2641 tggcgctcga tgtggccgac cagaccattg ccgaaccggg ccgcgcggtc cgccatacgc
     2701 gaagcggcaa ggagctgatc taccgccata gcctgctggt gcggttggcg cattggatca
     2761 acgtgctgtg cgtggtgctg ctcctgatga gcgggctgca gatcttcaat gcccacgcga
     2821 tgctgcattg gggcatcaag ggcgatgagg cggaccccgc gatcctgtcg atctatgccg
     2881 aggagcagct tgaggggccg ccgatcggct gggttcagat cgggaccgcg aaattcaaca
     2941 ccacgggttg gctcggcgtc ttccgcgacc gcgacggatt tctatccggc cgcgcctttc
     3001 cctggtggct caccatcccg agctatcaga atctggccga aggcagacgc tggcatttct
     3061 tcttcgcgtg gctgttcgcg ctgaacgggc ttgcctatct cgtctatgga tttgtggccc
     3121 ggcacttcca gcgcgatctt gcgcccacgc gtttcgattt tgcccatatc ggacgctcga
     3181 tcctggatca tctgacgttc aagcatccgc gcggcgaagc cgccaagcgc tacaatgtgc
     3241 tgcaaaagat gacctatatt gcggtcatct tcgttctgct gccgttgatg atcgccaccg
     3301 gcctcaccat gtctccgggc gtggattcca cggcgcattg gcttttggat gtgttcggcg
     3361 gccggcaatc ggcccgcacg attcatttct tcgccgcaac cctgatcgtt ttgttcatcc
     3421 tcgtgcacct gatcgaagtg ctcatctccg gagtcttcaa cgaattgcgc tccatgatta
     3481 ccggctggtt cgccgtgaaa ccggaaaaac caggcgcgaa aagtccagaa gagagggcca
     3541 agccatgaca cgtgaactca tcacgcgccg cgccgcgctt acgggcgttg ccgggacggc
     3601 tggattgatc ctttcgggct gcgaccggct ttcccagaac cagacgttcc agcgcatgct
     3661 cgaagccgcc gagaaactca atctcggcgg gcagcgcctg gtgctcaatt cctccgcgcc
     3721 gctcgcgcgc gaataccgga aatccgacat ctcgcgcgtc ttcaaggcga atggcacggt
     3781 ccgtccggac ggcgaggaat atcggcgcat ggcattcgag gaattccaga actggaagct
     3841 tcagatcggc ggcctcgtga acaagccgct ttcgttttcc cttgaggagc tgagggcgat
     3901 gccgtcacgc acacagatca cgcgccatga ttgcgtggag ggctggagcg cgattggcga
     3961 atggacgggc gtgccgcttt cggctttgct caaccaagcc gggttgaagc ccgatgcgcg
     4021 ctacattgtg ttccgttgcg ccgacgatct catcggatcg cgcgacggca gcggcctgta
     4081 ttatgagagc atcgatctgg tggatgcgtt ccacccccag accatcatgg cgtatgcgat
     4141 gaacgggaat atcttgccga tcgagcacgg cgcaccgtta cggctgcggg tggaacgcca
     4201 gctcggctac aagatggcga aatatgtgat gcgtatcgag gcagtcgagg atttctccaa
     4261 aatcggcatc ggcaaaggcg gattttggga agaccgcggc tatgaatggt acgcggggat
     4321 ttgattctcg tcgcatcaaa cgagcttttg cccgcccatc accgccgcga tatcggcacg
     4381 cgccttgggg gatcgccggt aatattggtg agagcttccc gggtcgcggt cataatcgtt
     4441 gaatgacgaa caatcgacca tccggaattt cgcgggcgga aacaggttct ggtcgacgcg
     4501 gtcgtgcggg ccctcctggc cgagcctctt cgcgcccaaa ttgacggcca tgctcaggct
     4561 caggaccgcg tccgccgtgc tgtaataaac ggaggtgcgt ttcgtaagct gcttcaggtc
     4621 gcacaatcgg ccgggagtcg gaaagctgaa actgtcataa cgctcgtcgg cggcgcaaag
     4681 aaacgcttca tcgaacagat ccgcggcgcc gttcccatgc aggaaccaac tctccacggc
     4741 ggcttgcagc gcccaattgc ccatactgtg cgccaagagg aaactgcgtc gccccgattt
     4801 tcgcaccgcc gtgacgatcg gttccagatt ggcaaagaaa ctcatgatgt gcggcccgga
     4861 ttggccggca acggtttggt catgaatata atcgctccac agaatgggaa acgagaccag
     4921 cttgccgagc gacggccaac tgaaagagat tacggtcgtg ttggcgcccg gcgcgccgga
     4981 ctgggcgaac cattgctgat tgaaggccgc gcgcgtgatc gcgttttcaa agttgttgtc
     5041 gaaaccgtga atgaatatca acagattgcg gcccggggtc gacagatcgg cgagcgcctc
     5101 gggactaaat cggcctttcg agatgctctg gatggatttg atggcgccgg tcgtgtccgc
     5161 cgtcagattc gcatcctcca cgaaggccgt ggcgtaggtg atctggttcg gatcggtcgg
     5221 cgagacaatg gagtcgccat agttgcgata atcgctggcc gcgccggaaa tgacgcgatt
     5281 ggtcgcaaaa taaaccgttg tagtcatcgg atgctcctgc cagctaaagc tggggtatta
     5341 tagaggggaa accgcgaggc gatgccggat tctctcaacg tcctcaccat ggcgcccgca
     5401 ttcgtgatat ccaaccggct ataatgcgtc ctgtctctcg cttgtgttag ggttcgccgc
     5461 ctaaaaagcc tgatcgaatc ggcgcggggt acgcgcgcgg ctcgcagcac gggagagatt
     5521 tccatggcgg tgacactcaa ggtcaacggc aagacgcacg cactcgacat cgagcccgat
     5581 atgccgctgc tctgggcgct gcgcgacgag atcgggctga caggaaccaa gttcggctgt
     5641 ggtatcgcgc aatgcggcgc ctgcaccgtc cacatcgacg gacaggcgat gcgctcgtgt
     5701 tcggtgccgg tttcgaccgc ggccggtaag gacatcacca ccatcgaggg gctcgcggcc
     5761 gccgatggca agctgcatcc cgtgcaggcg gcctggatcg ccgaggatgt gccgcaatgc
     5821 ggctattgcc agtcgggcca gatcatggcg gcggtagcct tgctcaagac gaagcccaat
     5881 cccacggacg aggatatcaa cggcattacc aatatctgcc gctgcggaac ctatatccgt
     5941 atccgccgcg cgatccatcg cgccgcgacg accaaagcct aggatcggga gatatccatg
     6001 ggacatgtcg aaccgattgc ccgtgacgag gctttgtcac gccgcaaatt catcgtcacc
     6061 ggaatgtcgg cgggcggcgg tctggcgctc ggcttcgcgt ttccggggct cgcatcggcg
     6121 cgtccgctgg gcgaagcgcc gtggagcaac gatgcgctgg ccgtctccaa cgaggtcaac
     6181 gcctggatcg tgatcgatcc ggacgaaacc atcaccatcc gcattccgca ttccgaaatg
     6241 gggcagggcg cgggcacctc caatcccatg cttgtggccg aagaattgga atgcgattgg
     6301 gccaagatca agggcgagtt cgcgtcgccc aaccgcaatg tgcgcgagaa caatgtctac
     6361 aaggaccaga acacggtcgg ttcgcgcggc gtcgcgacat cctggcaata tctgcaacag
     6421 gccggcgcga gcgcgcgggt gcgcctcgtg caggccgccg cccatcaatg gaacgtgccg
     6481 gcgtccgaat gcgtcgcgca gaatggcgcg gtcattcaca cgctgacgaa acgtacgctg
     6541 acctatggca agctcgcagg cgatgcggcg aagatcacgc tggacaagga accggcgatc
     6601 aagacaccgg atcaatacaa gctggtcggc aagccgcttg cccgtctcga tacgccggtc
     6661 aaggtcgatg gccgcgccaa atacggcatc gataccaaag tgccggacat ggtctatgcc
     6721 gcgatcatct cctgcccggt ggtcggggga acggtcgcga gcgtcgatga aagcggcctc
     6781 gccggcaagc gcggcatcgt caaggtggtg aagctgaaag acgctgtcgc cgtcgtcgcc
     6841 gacaatacgt ggcgcgcgat gcgcggcctg gatgcgctca agatcgattg gaattacggc
     6901 ccggccggta ccagcgacag cgcgcagatg cgctcgatgt atcgcgccac gctggacgaa
     6961 ccgatgatac aggcgcgcca tgacggcgat gcgccgggcg cgttggccaa cgcgggcaag
     7021 atcatcgact ccgtctatga ggttccccat ctcgcgcaca cgccgatgga gccgttgaat
     7081 gcgaccgcgc attttcaggc cgaccgggtc gatgtctgga tcggcacgca aactgcgctc
     7141 ggcacgctcc agcaaggcgc tgccgcttcg ggccttaagc ccgagcaagt ctatgtccac
     7201 aacgcctatc tcggcggcgg cttcggccgg cgctccaaga acgacgaaat ggaacaagcc
     7261 atcgccatct ccaaagaggt cggcaagccg gtgaagctga catggagccg cgaacaggac
     7321 gtccgtggcg accgctatcg gccacaggcg gccgcgcggt tcaaggccgc gctggggccg
     7381 gacggcaaac cctccgtgat cgatgcgcgc gtcgcggtcg gttcgatcaa tcgttcgctc
     7441 gggcgtcccg tcgatggcag catcgaaggc caggcggtcg atggcatcgt caattccgct
     7501 tacaaaattc cgaatttcta tgtcggcggc gtgatgaaga acacgcattt gccggtgagc
     7561 ttctggcgct cggtgggcgg ctcgcagaat tgcttcttct acgaaagctt catcgatgag
     7621 ctggcgcacg cggcgggcaa ggatccctat caattccgcc gtgacatggt cgaccgggtc
     7681 gacttcgtcg gtgtccttga gaagctcgcc gaaaagagcg attggaaaca ggcgctgcca
     7741 aagggccgcg gccgtggcat cgcagtggcg gaaaatcacg gttcggtggt cggcaccgtg
     7801 gccgaagtca cggtcgacgc ccaggaccgc attcgcgtgg accgcatggt tgtggcggtc
     7861 gattgctacc acgtggtcaa cccgagcatc atcgaggcgc agatgcagtc tggcgccatc
     7921 ttcggcatga ccgccgcgtt ctatggcgag atgacggtga aggacggtca ggtgcaggaa
     7981 ttgaacttcg acacctaccg gatggtgcgc ctcgccgagg ctcccaagat cgaagtctat
     8041 ctggtgcctt ccggcggccc gaaattcggc ggcatcggcg aatgcgggac cgcgacgatc
     8101 gcgcctgcca tcaccaatgc ggtgttcgcc gcgaccggca agcgcgttcg ccaattgccg
     8161 ctcaaaaata tcaagctgtc gcagctcgcc agtctgtagg ggcggcattt tctgtagcgg
     8221 cggcgtttcg ccgaaaaacc ccccacaccc cgcgcgtggg gggtattttg tggagctttg
     8281 gcctttccag gaaaatgcta tcgtcggttc cgcaggggga ccgagactcg acgatggacg
     8341 atttcttcgc ggcgctgagg gaccgccatg tctatcgtgt ggcggcggcc tatgtggtcg
     8401 tcgcctggat actgctccag ctttacaaca atttgcagcc catcctgaag ctcgcggact
     8461 gggtcgggac attggtgctt gttgtgctcg tgacgggttt cccggtctcg ctcctgctgg
     8521 cctgggtcta tcagatcacg cccaattccg gcgcatggct gtctcagcgt tcggccaaga
     8581 caccgccgct ttttcagccg aagtccaccg gcggcatttc catcgccgtc ctgccattgg
     8641 caaatctgtc tggggattcg acccaggaat tcttttccga cggaatgacc gacgagatca
     8701 catcggcgct cgccaagata cgggatttgc gggtcgtcgg acgttcctcg gcgttccagt
     8761 tcaaagggca atacaaggat ctgcgcgcca tcggacgcgc gctgagcgcg cgttacctga
     8821 tcgaaggctc ggtgcgcaaa gccggggacc gggtgcgcat cacagcacag ctcgccgaag
     8881 ccgagagcgg gtcgcagcgc tggtcggaaa cctatgatcg cgaactcaag gacgtgttcg
     8941 ccgtgcagga ggatatcgcc cacgccatcg ccgcggccct gcatgtgtcc ctcggcctga
     9001 agcgcggcga atcgctggtg ccgagccgca cgtcccatct cgattcctat caggattatc
     9061 tgcgggccaa ggcgctggtg agggcgcgcg gcccgcgcga gtccggaggc ccgcttacgg
     9121 aagcggcaaa attgctggag caggcggtcg cgcgcgcccc gaactatgcg ccggcctggg
     9181 ccgtactggc gcaggcttac agtttgatcc ccgctttttc tccgcccccg accggcggtt
     9241 ccatcgcgga atatcgcagc cggatcatgg aatcgctcac caaggcggaa acctcggcgc
     9301 agcgcgccat tcggctcgat ccctcccatg ccgagggcta tatcgggctg gcgacggtgc
     9361 acacgttccg ccgccaattc atcccggccg aagacctgtt ccagaaagcg ctgtcgctcg
     9421 atcccaacaa tccggacgcg ctgcagatct acagcgtcgc cctcgcgacg gccggccgga
     9481 tcaaggatgc gcttcctttg cgccttcgct tgcgcgcgct cgagcctttc gttcccgtat
     9541 tcaacgcctt cacggcgcaa atcctctggc tcaacggaca ggacgatgcc gcaatcgcga
     9601 tactgaaagc gctgccgcgg aataccgcct tcgccgcaat ttcccttgcc cagattcacg
     9661 cggccgcggg acgctatggg gaagccgccg atgcattgct ggaggcggcg ccggggttct
     9721 atcgccccgg ggcggtggaa atcgccgcgg gtttgttgcg caccgccccc gtgcacgccg
     9781 tctcggttga aagccttccc aaactcggtc cgctcagttt cgtttatctt tatgccggtg
     9841 cgcccgaacg cgccctcgaa cctgccgaag atggcgatcc cgatgcgcca acggcaatgc
     9901 tgttgtggca cccttcggcc gcagccgcgc gcaagacggt gcgtttcaag actttggtgc
     9961 acggaacggg aatggtcgaa ttctggcgcg cgcgaggctg gcccgatctc tgccgcgcgc
    10021 aaggccccga cgatttcgtc tgcgattgaa tcgccccgcc atttccggcg catgaaaatc
    10081 acagccatct tcccgatcgc cggttgaggg cagcgccgca tttgactaac tttctttagc
    10141 catgcgaatc gagcgcgttc tgtgaacgga attggcccgc gggaccgggt cagacttgcg
    10201 gtcaggatcg ccattccggc cattctgacg gcagccctga gcgcctgcgt caccaagccg
    10261 gttccgcgca tgccggcccc ggtcgtgaat cacgcgcccg ttgccgccgc atccccggcg
    10321 ccgccaccgc cggcgatgac ggcccccaac ccgatttaca aggtcggtaa tccctatcag
    10381 gtggcgggca tctggtatta tcccaaagaa cagccggatt atgacgagac gggaatcgcc
    10441 tcctggtatg gagcggattt ccacgggatg ctcacggcca atggagaggt gttcgatcgc
    10501 aacgcgatct ccggtgccca tccaacgctg ccgatgcccg tcaacgtgcg ggtcaccaat
    10561 cttcagaacg gacgctcgct cgtggtgcgg gtcaacgatc gcggtccctt catcaacggg
    10621 cgtgtgatcg atctttccga acatgcggcc gatctccttg gcttccgcac aaccggcacg
    10681 gcgcgcgtgc gcgtgaccta tctcggccgc gccgatctct tcggcccggg cctcgcgccg
    10741 ccgtcgcagg agacgccgcc ggagatcgcc atggcggttc ccgcggtccc ggtgacgggt
    10801 gtcgatgcca cccccctgcc gccggtgatg ggggccaagg tggaaccgcc gccgccgatg
    10861 gtgagccttc ccaaacccgt tcaacacgtc gaaattgctc agctcgatgc ggtcgcggat
    10921 gggcatgtca ccgagatggc agtccccaag gcgacggcga tctatgtcca ggcgggagcg
    10981 ttcacatcgc ccacgaatgc gggcgccgtg gcggcaaagc ttcagagcac gggcgcgcgc
    11041 gtctatcccg gacgcaagga tggccggccg atctaccggg tccgtatcgg gccgtttcag
    11101 gacgtcggcg cggccgattc agcgctcgtc caggttcaga ccctcggtca tagtgacgcc
    11161 cagatcgtgg ttgattctgt ctcgagctga agaagcggcg cgggactcgt cgaatcgccg
    11221 tttttcgtgc gttgttgcgt acctcaggga ggggtcgttc gatgtttttt tcggatcatg
    11281 ttcgcccatt ggcttttggg cttgccgcct gcgcaagcgt gaccgcgccg caagccgttc
    11341 aagccgagat cctcacaacg gccaagcagg ccatcatcct cgattatgac agcggcgaga
    11401 cgctgtactg caaatcgtgc gaagagccga tgccgccgtc ctccatgagc aagctgatga
    11461 cggtggaact cgtcttccag cgattgaagg acggcaggct gaagcccgaa gacacgttcc
    11521 atgtgtcgga aaccgcatgg cggcagggac agaaagacaa tgaatccaaa atgtgggtcg
    11581 cgctcaattc ggatgtggct gtcgacgatc tgttgaaggg catcatcgtg caatccggcg
    11641 gcgacgcctg cgttgtggtc gctgaagcgc tcggcggttc ggaatcgggt ttcgcggata
    11701 tgctcaatgc ccgcgcaaag gagctgggcc ttgcgcattc gcatttcgtg aattccagcg
    11761 ggcttcccga ccccggccaa ttcatgtcgg cggaggacat cgcgaaactc tccgcacata
    11821 tcgtgcgcga ttatccggat tattataaat atttcgcgat tcccgatttc acctggagca
    11881 agatcaagca ggcaaaccgc aacacgctgg tcgatgagaa tatcggcgtc gacgggttga
    11941 agaccggaca taccgaagtc ggcggctacg gcattgtcgc gtcgtccttg cgcaatgggc
    12001 ggcggctggt cctggtgatc aacggaatga aaagcgagcg tgagcgcatc aacgaggcgc
    12061 gccggctgct cgatatcggt tatcgtgaat tcaaggatta caagcttgcc gacaagggcg
    12121 ctgtgctggg cgatgcccag atctggggcg gcgcgaagaa cgcggtcggt gtcacggtca
    12181 aggacccgct ggtcgtgatg ctgccggtgg attcgcggcg cgacatgaag gtgacgctgc
    12241 gctatgacgg ccccgtgcgg gcgccggtcg ttgcgggcca gcagattggg accctgaccg
    12301 tttcggtgcc gggaaaaccg gataaggtgg tgccggcggt ggctatcgaa tccatgccgt
    12361 ccaactcgtt cctcgataag atgatgatcg ggctccagac gctgatcttc ggcgccgaca
    12421 aggcgtgaaa gaccaagatg tgaatggttc cgggaaaatt catcagtctc gaaggcggcg
    12481 agggggccgg aaaatccacc caggcgaaat tgcttcgttc cgcgctggaa gcgcgcggcc
    12541 atagcgttct tttgacacgc gagccgggcg gctcgccggg tgcggaggaa atccgcaaat
    12601 tgctggtgga gggtgagccg gagcgttgga ccccgctggg agagacattg ctgttcctgg
    12661 ccgcgcgtgc cgatcacgtc acacgcgcaa tcgcaccggc tcttgcgtcc ggagcgtggg
    12721 tgatctcgga ccgcttcgcg gattcgaccg tggtctatca gggcattgcg cgcggtctgg
    12781 ggatggcgcg cgtgcgcgcc ctgcaaatgg cggcgcttgg cgattttatg ccggatctga
    12841 cacttgtcct cgacgtgaac ccacaggatg gtctggcacg cgccggcgcg cgcggagcgg
    12901 cggagaatcg cttcgagcgc ttcgacgatg cgttccatcg ccgtctgcgc gacggttttc
    12961 tcgcgatcgc agccgaagcg ccgcagcgct gcgcgattct cgatgccgcc ggaacggtag
    13021 acgccgtcgc cgccaaaatc tggaacatcg tcgcggagcg tttgcgccca tgagtccggc
    13081 acgccgcgag accgaaatcg aggaatccga ccgcatcgaa ggtcaggcgc atccgcgtga
    13141 aacctatgcc ctcgtgggac aggacgccgc actttcgcgc gtggctcgcg ccattcgttc
    13201 ggcccggccg cctcaggcct ggctgatcgc cgggccgcct ggcatcggca aggcgacgct
    13261 ggcctatcgc atcgcacgct atctgttgcg ctatggcgcg ggcgcggccg gtcctgccga
    13321 tctctgcgtc cccgcgaacg accccgtctc gcgtcagatc gaagcggggg cacatccggg
    13381 cctcctggtg ttgaagcggc agttgaacga gcgcggcaag ctcaggacgg ttctcgatgt
    13441 cgctgagatt cgacgtctcg gcgggttctt tggcatgacg gccgaagccg gcggctggcg
    13501 cgtggccatc gtcgattccg ccgatgagat gaacgacaat gcggcgaatg cgctgctgaa
    13561 gattctcgag gaaccgccgg ggcgcggtct tctgcttctc atcagccacg cgcccggcag
    13621 attgcttccg accatccgtt cgcgtaccca gcggctcgat ctgaaaccgc tgtccgattc
    13681 cgtcctggac gaagcgctgg tgcgccttct tcccgatggg agtagcgatg accgcgcggt
    13741 gctcgtgcgt ctggccgaag gctcccttgg gctggcacta cggctcagca acggcgatgg
    13801 gctgaaactc gcgagcgatg cgcaagacat gatcgcggtg cgcggcgcac cggattttcc
    13861 ggcggttctc gctatggccg agcgtgtgtc gcgcgccgcg gacgggttgc agcatttcgg
    13921 cgcatttctg tcgcaggcga tttcaagccg catccgcgag cgcagccgaa gggccgaggg
    13981 cgacgaccgc gcggtcgaaa tgtgggaacg cctgaacgcc ctttatgagc gcgccaccgg
    14041 cctgcatttg gaaccgcgcc agacgattct tgcctcggca cgcgaaatcg ccggtgcgaa
    14101 acgccgcggc gcgttgtaga acgcgaacat gcttgtcgac agccactgcc atctggattt
    14161 tcccgatttt ggcgcggaac tcgattccgt tcttgcccgc gcccgcgcgg ccggggtggg
    14221 acatttcctc accatcggga cagagctgaa gcgatttccc ggagttctcg cggcggccga
    14281 aaccgcagcc gacatccatt gcaccgtcgg cgtgcatccg cacgaagcgg cggaggaacc
    14341 gctttgcggt cccgatgtgc tgctgaagga aagccggcac gcgaaggtcg tcggcttcgg
    14401 cgaaagcggc ctcgattatt attacaatca cagtccgcgc gaggcgcaga ttgccaattt
    14461 ccgcgcccat atcgcagcgg cgcgcgaagc caagctgccg ctcgtggttc acacgcgcga
    14521 cgcggaagac gacaccatcg cgatattgcg cgaggagcgg gagaggggcg cttttaccgg
    14581 cgtgatccat tgcttcaccg gcaccgcgcg cctggccggt gccgccttgg agctggggtt
    14641 ctatatttcg gtttccggta tcgcgacgtt caagaaatcg gacgtcttgc gcgcgactct
    14701 gaaagaggtg ccgctggacc gtctgctggt ggaaacggac gcgccctatc tggcgcccca
    14761 gccggcgcgc ggcaagcgca acgagccggc cttcatggtg catacggccg cgatgctggc
    14821 ggagctgaaa gacgttgcgc acgcagacat cgcacgcgcc accacggaaa atttcttccg
    14881 cctgttcacc aaggctggac ccgcgtgatc gcgggcgatc gtctttgcgc gaccatcctc
    14941 ggctgcggct cgtccgccgg cgtaccgcgg cttggcggcc cgaacggcgt gggcgattgg
    15001 ggagcgtgcg atccggccga tccgaaaaac cgccgccggc gctgttcgct gctggttgaa
    15061 cgtgggcgca cggcagtgct cgtcgacacc tcgcccgaca tgcgcgaaca attgctggcc
    15121 gcgcgcgtga tccatctcga cgccgtcctg atgacgcatc cccatgccga tcagacaaat
    15181 gggatcgacg atttgcggcc gctgacattt ctgatgaaaa gacgcgtgga catgtacgcg
    15241 gatgaagcag cactcgcgca tctgtcgcgc caattctcct attgtttcga gacgccggct
    15301 ggcaccgaat atcctcccat catcaccggc catgtcatcg ccgagccgtt ttcaccattc
    15361 catgtgaacg gggaaggggg cgccattccg gttgtggcct tctggcaggg gcatggaccg
    15421 gtgcgatcgc tgggcttccg ctccggcggg ctggcctatt ccagcgatgt cagcgatctc
    15481 gatgccgccg cgttcgccgc tctcgaaggc gtggaaacct ggatcctcga tgcgctgcgc
    15541 tatacgccgc atccgacgca cgccaatctc gagacggcgc tggcctggat cgcgcgcgtc
    15601 aagccgcggc gcgcgatcct caccaatctg cacatcgaca tggattatgc cgaactcgcc
    15661 gcgcaattgc ccgccggcgt cgaaccggcc tatgacggaa tgattgtcga agttccgctc
    15721 tgaattggat cagctccaga gcgcgtgtga ggcaaggcgc gcgccttccg aa
//
LOCUS       DASYEG010000210        13352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4846502, whole genome shotgun sequence.
ACCESSION   DASYEG010000210 DASYEG010000000
VERSION     DASYEG010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 13352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13352
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4846502"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /gene="radC"
                     /locus_tag="VGM72_09415"
     CDS             <1..295
                     /gene="radC"
                     /locus_tag="VGM72_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEY4265525.1"
                     /translation="LFLDRKNVLVADEVHQRGTVDHTPVYPREVVKRALELGASAIIL
                     VHNHPSGDPTPSRSDIAMTREIAAAAKALKIEVHDHLIIGRGKHASFKALGLL"
     gene            525..1694
                     /locus_tag="VGM72_09420"
     CDS             525..1694
                     /locus_tag="VGM72_09420"
                     /inference="COORDINATES: protein motif:HMM:NF023802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain-like cysteine protease family protein"
                     /protein_id="HEY4265526.1"
                     /translation="MELSSLFSASRLRYGQSTIAQEIGDWLFESTALNFNMQAQTQTN
                     WCWAATSTSVSHFYWSASTWTQCSVASAELGRTDCCNASVPSACNVSWYLDRALTRTS
                     NLQSMVTGTIGFDAILAELRAGRVVGARIGWSGGGGHFMCIYGCSRVIGTEYVDIDDP
                     IYGKSHITLATFTSNYQGSGSWTHTYYTKRYPILHIRLPILEAVLLEKIREARPLLAV
                     KRGSQDFQTVLAPAADLAVPHHVYVAGLHDLTQKENPLPANPTAMRVFEVTGDRNTAL
                     FDVSLPEQGPAQVLSMSDDAASLDLLTRGLTEISRMAARGEGEPELRFLRIPALYVEA
                     FWLHYPDSAKDVMVPVRGIGLFTPFQPVAEQQFLDAVRKAARERSHGAPDDTIAP"
     gene            1795..3261
                     /locus_tag="VGM72_09425"
     CDS             1795..3261
                     /locus_tag="VGM72_09425"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY4265527.1"
                     /translation="MLKLDDNALLRDAAYIDGQWRGAASSARFAVKNPATGEVIAEIA
                     DCAEDDVRIAIEAAHRAWAPWRAKTAKERALLMRRWYELMMAAQEDLAQLMTAEQGKP
                     LAESRGEIAYGASFIEWFAEEGKRIYGDVIPTNAPGRKILVMKEPVGVVAAITPWNFP
                     NAMITRKVAPALAAGCPAVVKPAQLTPLSALAIAELAHRAGIPKGVFNVVPSTQSARV
                     GKELTENPLVRKFSFTGSTEVGKQLMRQCASTMKKISLELGGNAPFIVFDDADLDAAV
                     KGAMASKFRNMGQTCVCANRFLVQDGVYDAFAEKLAKAVSALKIGDGMIAGVEQGPLI
                     NMAAVEKVEHLLEDATAKGARIAAGGHRHRLGGTFFEPTIVRDVTPAMDFAREEVFGP
                     VATLFRFNDEADAIRLANDTEYGLAAYFYARDVGRIFRVTEGLEYGIIGVNEGIISTE
                     VAPFGGMKESGVGREGSKYGIESFLEIKYVAMGGLGMA"
     gene            3286..4593
                     /gene="purB"
                     /locus_tag="VGM72_09430"
     CDS             3286..4593
                     /gene="purB"
                     /locus_tag="VGM72_09430"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY4265528.1"
                     /translation="MIPRYAREPMTAIWSPQTKYRIWFEIEAHAADAMAELGIIPKES
                     ARIIWEKGRNANFDVERIDAIEREVKHDVIAFLTHLAEFIGPEARFVHQGMTSSDVLD
                     TTLAVQLSRSADILIADVDGLLAALKTRAFEHKFTPTIGRSHGIHAEPTTFGVKLAGF
                     YAEFARARARLVAARAEIATCAISGAVGTFANVAPVVEDHVAAKMGLAVEPISTQVIP
                     RDRHAMFFSTLAVVASSLERLATEIRHLQRSEVGEAEEYFSPKQKGSSAMPHKRNPVL
                     SENLCGLARMVRAYALPALENVALWHERDISHSSSERYIGPDATITLDFALARAAGLI
                     ERLVVYPERMLANLERTGGLVHSGRVLLALTQKGMAREDAYRLVQENAMRCWRGEGNL
                     IDLLKANTDVTHLMSSPELAECFDLAYHLKEVDRIFTRVFGAH"
     gene            complement(4808..5140)
                     /locus_tag="VGM72_09435"
     CDS             complement(4808..5140)
                     /locus_tag="VGM72_09435"
                     /inference="COORDINATES: protein motif:HMM:NF018993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HEY4265529.1"
                     /translation="MSGSFEKREKGFEAKWAHDEELRFKVYARRNKLLGLWAASEMGV
                     TGPAADSYAKEVIAADFERAGDEDVFEKVRRDFDARAVALSDHMLRRKMEDLLDVAKY
                     QIETEITK"
     gene            5432..6193
                     /gene="purC"
                     /locus_tag="VGM72_09440"
     CDS             5432..6193
                     /gene="purC"
                     /locus_tag="VGM72_09440"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY4265530.1"
                     /translation="MKRRRTIYEGKAKILYEGPEPGTLIQYFKDDTTAFDAKKRAVLE
                     GKGVLNNRISEYLMTQLNGIGVPTHFVRRLNMREQLIKEVEIIPLEVVVRNVAAGSLS
                     TRLGIAEGQALPRSIIEYYYKNDALHDPWVSEEHITAFAWASPQDLDDIVNLTIRIND
                     FLSGLFLGVGIKLVDFKIEFGRLWENDFMRVVLADEISPDSCRLWDIQTNEKLDKDRF
                     RRDLGGVTEAYSEVARRLGILPESTQGGTGPVLVK"
     gene            6190..6432
                     /gene="purS"
                     /locus_tag="VGM72_09445"
     CDS             6190..6432
                     /gene="purS"
                     /locus_tag="VGM72_09445"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008033785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEY4265531.1"
                     /translation="MKARVFVTLKSGVLDPQGKAIGHALNNLGFDSVGDVRQGKVIDI
                     ELAEKDETRARARLKEMCEKLLANTVIEKYEIELKP"
     gene            6429..7097
                     /gene="purQ"
                     /locus_tag="VGM72_09450"
     CDS             6429..7097
                     /gene="purQ"
                     /locus_tag="VGM72_09450"
                     /inference="COORDINATES: protein motif:HMM:NF002957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEY4265532.1"
                     /translation="MKSAVIVFPASNCDRDAQTALADLTGTKPFMVWHGDTEVPACDL
                     IVLPGGFSYGDYLRCGAMAAQSHIMRDVKKKAEAGVAVLGICNGFQILTETGLLPGAL
                     MRNADLKFVCRPVNLVVEESQSAFTRHYSKGEQVRFPVAHGEGNYFADDGTLDRLEGD
                     GRVLFRYAPGDNPNGSARDIAGILNEKRNVLGLMPHPERACDPLLGSTNGRALFQSLI
                     ETLS"
     gene            7110..9305
                     /gene="purL"
                     /locus_tag="VGM72_09455"
     CDS             7110..9305
                     /gene="purL"
                     /locus_tag="VGM72_09455"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEY4265533.1"
                     /translation="MTQITPQIVREHGLSDSEYARIKQLLNRDPNLVELGIFSVMWSE
                     HCSYKSTRVHLRKLPTKAPWVLQGPGENAGVIDIGDGDACIFKMESHNHPSFIAPYQG
                     AATGVGGIMRDVFTMGARPIAMMNALRFGEPAHPKTRHLISGVVAGIGGYGNCMGVPT
                     VGGEVNFHRSYNGNILVNAMCVGLARSDRIFLSAAKGAGNPVVYIGSKTGRDGIHGAT
                     MASAEFNEDSEEKRPTVQVGDPFTEKLLLEACLELMATDAIIAIQDMGAAGLTSSSAE
                     MGDKGGSGIELDLDKVPQRETNMTAYDMMLSESQERMLAVLKPGREGEAEAIFKKWEL
                     DFAVIGITTDTKRLVVKHKGGVVADMPITALSDAAPVYERPHEKRAPARSAPMFDATR
                     PVMESLMTILASPDMCSRRWVWEQYDNSVMADTIQMPGGDAAIVRVHGTNKALALSTD
                     VTPRYCRADPFEGAKQAVAECWRNLTAVGALPLAITDNLNFGNPERPETMGEIVASID
                     GIGEASRKLSFPVVSGNCSLYNETNGEPILPTPAIGGVGLIKDLSRMASIALKREGDA
                     ILLIGETKGHIGQSVYLREIEGREEGPAPSIDLAAEKRNGDFVRGQIEQGRVDTVHDL
                     SDGGLLVALAEMALAGGIGVALDLDTDIALLFGEDQGRYILSAPVIAASSILEQAKAA
                     GIAASLLGRTGGDKIILEGADEISLSKIRSAHEGWFPAYMSGGELPPTN"
     gene            9317..9550
                     /locus_tag="VGM72_09460"
     CDS             9317..9550
                     /locus_tag="VGM72_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEY4265534.1"
                     /translation="MAMPAKDIELAIRAALPDADVTITDLAGDGDHYAAMVKSAAFKG
                     KTRVQQHKMVYDALSGRMGGQLHALSLQTSAKD"
     gene            9618..9956
                     /gene="grxD"
                     /locus_tag="VGM72_09465"
     CDS             9618..9956
                     /gene="grxD"
                     /locus_tag="VGM72_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEY4265535.1"
                     /translation="MSQTSVHDQIAKDLAENEVLLYMKGTPVFPQCGFSAATVGVLSE
                     LGVKFKAVDVLKDPEIRAGIKEFSNWPTIPQLYVKGEFVGGCDIVKEMFEEGELEAYF
                     KEKGIALQNA"
     gene            complement(10009..10626)
                     /gene="rpsD"
                     /locus_tag="VGM72_09470"
     CDS             complement(10009..10626)
                     /gene="rpsD"
                     /locus_tag="VGM72_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY4265536.1"
                     /translation="MTKRTTSKYKLDRRMGENIWGRGKSPINKRNYGPGQHGQRKSRK
                     LSDYGTQLHAKQKLKGYYANITERQFRGYYEEASRKTGDTGEMMIGLLERRLDAVVYR
                     AKFVPTPFSARQLVNHGHVSVNGKRVTIPSYLVKEGDLIELRSKAKDMALVLEAVKSP
                     EREVPDYIEADHGKMSAKFLRTPKLGDVPYAVQMQPSLVVEYYSR"
     gene            complement(10710..11483)
                     /locus_tag="VGM72_09475"
     CDS             complement(10710..11483)
                     /locus_tag="VGM72_09475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY4265537.1"
                     /translation="MPELVLAPVIILSEPQLGENIGACARAMANFGLSDLRLVKPRDG
                     WPNEKAQAMAVGAAGLIAAAQVYDSVPAAIGSLRLVYASTARDRAMAKPVLTPAEAAR
                     RLREAALAGIPTGLLFGNERAGLSNDEVALADCVITIPTDPSFSSINLGQAVLLMAYE
                     WFRSADATPTEQIDHGAALPAPRAELFRLFEHLEGELEKSGFLFPPGNRPGMIRNLRS
                     ILHRAQLTDQEVRTLRGVIVALTKGKRRNPPKPVPPKDV"
     gene            11608..12819
                     /locus_tag="VGM72_09480"
     CDS             11608..12819
                     /locus_tag="VGM72_09480"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY4265538.1"
                     /translation="MAKIKVANPVAELDGDEMTRIIWDLIKKKLILPYLDITLEYYDL
                     GVEHRDATNDQVTVDAAEAIRRLGVGVKCATITPDEARVKEFNLKQMWKSPNGTIRNI
                     LGGTIFREPIICKNVPRLVPGWTQPIVIGRHAYGDQYRCTDFLVPGPGKLTVAWTGKD
                     GKKIEREVFDFPGAGVAMTMYNLDQSIIDFARASMNYALMRKYPLYFTTKNTIVKAYD
                     GRFKDLFEKVFNEEFTAKFKAAGLTYEHRLIDDMVAACLKWSGGYVWACKNYDGDVQS
                     DTVAQGYGSLGLMTSVLMTPDGDTVESEAAHGTVTRHYRQHQKGQPTSTNSIASIFAW
                     SRGLSHRAKLDGNSDLTHFASSLEKVCVDTVESGFMTKDLALLIGPEAKSLSTEGFLD
                     KVDENLKKAMA"
     gene            complement(12824..>13352)
                     /locus_tag="VGM72_09485"
     CDS             complement(12824..>13352)
                     /locus_tag="VGM72_09485"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEY4265539.1"
                     /translation="RYLESQGEAAREAAAVLKTSAVELPSRIHALVEDRRRLERELSE
                     AKKSLALSGGPKAGAEAYKEIGGVKYVLRIVEDVAAKDLKALADEAKTKLGSGVVAFV
                     TVADGKASLVVGVTPDLTTRISAVDLVRAGADALGGKGGGGRPDMAQAGGPDGARAKD
                     ALSAIEQRLAALETT"
BASE COUNT         2678 a         4004 c         4022 g         2648 t
ORIGIN      
        1 gctgttcctg gaccgcaaga acgtgctcgt ggccgatgag gtgcatcagc gcggcaccgt
       61 ggaccacacg cctgtctatc cgcgcgaagt cgtcaagcgc gcattggaat tgggcgcctc
      121 cgcgatcatc ctggtgcaca atcatccgag cggcgacccc acgccctcgc gaagcgacat
      181 cgcgatgacc cgtgaaatcg ccgcggccgc caaggcgctg aaaatcgagg tgcacgacca
      241 tctcatcatc ggtcgcggca agcacgcgag cttcaaggcc ttggggctgc tttaacgccg
      301 tctgctttca acgacaaatt ttcgcgaagc tactccgatg ggcgcgagac atcgcactgc
      361 cggatgggct tggcgatcgg atttcgatgt caagtcagga gcttggcgtg caacagacgg
      421 ccttacccgg atttcatcaa acagattttg ttatcggccc tatttatggt taatatttcc
      481 gtctgcaaaa atttcaccgc aatgttttat ttcggagacg gtcgatggaa ctttcctcgt
      541 tgttctctgc atcgcgcctg cgatatggcc aaagcaccat tgcgcaggaa attggtgact
      601 ggctgttcga gtccactgcg ctcaatttca acatgcaggc gcagacccag accaattggt
      661 gttgggctgc gacatcgaca agtgtttcgc atttctattg gtccgccagc acctggacgc
      721 aatgttcggt cgcgagcgcc gaactcggcc gcaccgattg ctgcaacgcc tcggtccctt
      781 cggcttgcaa tgtctcctgg tatctcgacc gtgctctgac gcggacgtcg aacctgcaga
      841 gcatggtgac gggaaccatc ggcttcgacg cgatcctggc cgaattgagg gccggccggg
      901 tggtgggcgc acggatcggc tggtcgggcg gcggcggtca tttcatgtgc atatatggtt
      961 gcagccgcgt cataggcacc gaatatgtcg atatcgacga tccgatctac ggcaagagcc
     1021 acatcacgct cgcgacgttc acaagcaatt atcaaggcag cgggagttgg acccacacct
     1081 actacaccaa gaggtatccg atcttgcata tccgccttcc tattcttgag gctgtgctcc
     1141 tggaaaaaat ccgcgaggcg cgcccgcttc tggcggtcaa gcgcggttcc caggacttcc
     1201 aaaccgtgct ggcgcccgcc gcggatttgg cggtgcccca tcatgtctat gtcgcgggac
     1261 tgcacgatct cacacaaaag gaaaatccgt tgccggccaa tcccactgcg atgcgggtct
     1321 ttgaggtcac gggcgaccgc aataccgcgc tgttcgatgt ctcgttgccg gaacaaggac
     1381 cggcacaggt cttgtcgatg agcgacgatg cggcatcgct cgatctactg acccggggat
     1441 tgacggaaat ctcgcggatg gccgcgcgcg gcgagggtga accggaattg cgcttcctgc
     1501 ggatcccggc actctatgtc gaggcattct ggctgcatta tcccgattcc gccaaggacg
     1561 ttatggtgcc ggttcggggc attgggctgt tcacgccgtt tcagcctgtc gccgaacagc
     1621 aattcctcga tgcggtgcgc aaagccgcac gcgagcgcag ccatggcgcg cccgacgata
     1681 ctatcgcgcc ataatttccg ggagcagcgc aggcgccggg ccgttcgata ccgcgaggca
     1741 ttttgccttg cgtccgtccc gcgcgctaaa ttcgatcccc gaaaggaagt tcccatgctc
     1801 aagctcgacg acaatgcgct tctgcgcgat gccgcctata tcgatgggca atggcgcggc
     1861 gccgcgtcga gcgcgcgttt cgcggtcaaa aatcccgcaa cgggcgaagt catcgccgag
     1921 atcgccgatt gtgccgagga cgatgtcagg atcgcgatcg aagcagccca tcgcgcctgg
     1981 gcgccctggc gcgcgaagac cgcgaaggaa cgcgccctgc tgatgcgccg ctggtatgag
     2041 ctgatgatgg cagcccagga agacctggcc caattgatga ccgccgaaca gggaaaaccg
     2101 cttgccgaat cccgaggcga aatcgcctat ggcgcgtctt tcatcgaatg gttcgccgaa
     2161 gagggcaagc gcatctatgg cgatgtcatc ccgaccaatg caccagggcg gaaaatcctg
     2221 gtgatgaaag agccggtggg cgtggtcgcc gcgatcacgc cgtggaattt tcccaatgcc
     2281 atgatcacgc gcaaggtggc gcctgcgctg gcggcaggat gtcctgccgt cgtcaagccc
     2341 gcgcaactga cgccgctttc ggcactcgcc atcgccgagc tcgcccatcg cgccggcatc
     2401 cccaaaggtg tcttcaacgt ggtgccctcg acccaatccg cgcgcgtcgg aaaagagctc
     2461 accgaaaatc cgctggtgcg caaattctcc ttcacgggct cgaccgaagt gggcaagcag
     2521 ttgatgcgcc aatgcgcgtc caccatgaag aaaatttcgc tggaactggg cggcaacgcg
     2581 ccgttcatcg tgttcgatga cgcggacctc gatgcggccg tcaaaggtgc tatggcatcg
     2641 aagttccgca acatgggcca gacctgcgtc tgcgcgaacc gcttcctggt ccaggacggt
     2701 gtctacgatg ccttcgccga gaaactggct aaggctgtgt ccgcgcttaa aatcggcgat
     2761 ggcatgattg cgggcgtcga gcaggggccg ctcatcaaca tggccgccgt ggagaaggtc
     2821 gaacatctgc ttgaggatgc caccgcaaag ggcgccagaa tcgcggccgg cggtcatcgg
     2881 cacaggctgg gtggcacctt cttcgagccg accatcgtgc gggacgtgac gccggccatg
     2941 gatttcgcac gcgaggaggt cttcggcccc gtggcgacac tcttccgctt caacgacgag
     3001 gctgatgcca tccgcctggc gaacgacacg gaatatggtc tcgcggctta tttctatgcc
     3061 cgcgatgtcg gacgcatctt ccgcgtcacc gaagggctcg aatacggcat catcggcgtc
     3121 aatgagggaa tcatctcgac ggaagtagcg cccttcggcg gtatgaagga atcgggtgtc
     3181 ggccgtgaag gctcgaaata tgggatcgag agctttctcg agatcaaata tgtcgccatg
     3241 ggcgggctcg gtatggcatg acgtgcgcgt tctgacgagg ttgccatgat tccccgctac
     3301 gcccgcgaac ccatgacggc gatctggtcg ccgcaaacca aatatcgcat ctggttcgag
     3361 atcgaagccc atgccgccga tgccatggcc gagttgggaa tcattcccaa ggaatcggcc
     3421 cgcatcatct gggaaaaagg tcgcaacgcg aatttcgatg tcgagcggat tgatgccatc
     3481 gagcgcgagg tcaagcatga cgtcatcgcg ttcctgacgc atctggccga gtttatcggt
     3541 ccggaagcgc gcttcgtgca tcagggaatg acgtcgtccg atgtgctgga tacgacgctg
     3601 gcggtgcagc tttcccgcag cgccgacatt ctcattgccg atgtcgacgg ccttttagcc
     3661 gcgctgaaga cgcgcgcgtt cgagcacaaa ttcacgccaa ccatcggccg cagtcacggt
     3721 atacatgccg agccgacgac ctttggcgtg aagcttgccg gcttctatgc ggaattcgcc
     3781 cgcgcccgtg cgcgcctcgt tgccgccaga gccgagatcg ccacctgcgc catatcggga
     3841 gccgtcggca ccttcgccaa tgtggcgccg gtggtcgaag accatgtcgc cgcgaaaatg
     3901 ggccttgccg tcgagccgat ctcgactcag gtgatcccac gcgaccgcca cgcgatgttt
     3961 ttttccacat tggcggtcgt cgcaagttcg ctcgaaaggc tggcgactga aatccgccat
     4021 ctgcagcgct ccgaggtcgg tgaggcggag gaatattttt cgcccaagca aaagggctcc
     4081 tcggcgatgc cgcacaagcg caatccggtg ctgtcggaga atctgtgcgg gctcgcgcgc
     4141 atggttcgcg cctacgcgct gcccgcgctc gagaatgtgg cgctctggca tgagcgcgat
     4201 atctcgcact cctcgtccga gcgttacatc ggaccggacg ccaccatcac actggatttc
     4261 gcgctcgccc gcgccgccgg tctgatcgaa agactggttg tctatcccga gcgcatgctg
     4321 gccaatctgg agcgcaccgg cggattggtg catagcggcc gcgtgctgct ggcgttgacg
     4381 caaaagggta tggcgcgcga ggatgcctac cggctggtgc aggaaaacgc catgcgctgc
     4441 tggcgcggcg aaggcaatct gatcgatctt ttaaaggcga acacggatgt gacgcatttg
     4501 atgtcatccc cagaactcgc cgaatgcttc gacctcgcct atcacttgaa ggaagtggac
     4561 cgcatcttca cccgcgtctt cggcgcgcat tgagtctgcg acggatcacg cacatggcga
     4621 tgtccatcgt gatcctggca atgacgatgc tcggctgggc cggcgccaat taccgcggct
     4681 ggaccgtgat gttgttgggt gtgattccgc tgcccccccc tcgccgccaa gggtaccgag
     4741 tgggcgaagg ccgcaggcga tctccatata aatctgggct ggatgctgct cggcgtgtga
     4801 tccgagacta tttcgtgatt tcggtttcta tctgatactt ggcgacgtcc agcagatctt
     4861 ccatcttgcg ccggagcata tgatcggaga gcgcgaccgc gcgcgcatcg aaatcgcgac
     4921 ggaccttttc gaacacatcc tcgtcgcccg cgcgttcgaa atcggccgct atgacttcct
     4981 tggcatagga atcggctgct ggaccggtga cgcccatttc ggacgcggcc caaaggccta
     5041 acaacttgtt gcgtctggca taaactttga atcgcaactc ctcatcgtgg gcccatttgg
     5101 cttcaaagcc tttttcccgt ttctcgaacg acccggacat ttacataacc ctctgataaa
     5161 acagaattat ttcactatct tcagcaagct ccaggcgaca gtagcgcgag atggagacac
     5221 gtgcgacacc cgtttcgtca cacggcttgt cctgagggtt aagttgccgg aaggggcgcg
     5281 catccgctaa gttcgactct gacggggttg tcgcctttgg acgataagcc ccgttgccat
     5341 ttcggcgcga acgcgtatca cgggacccgg ctttttgccg aaaaccggcg agcctgacag
     5401 ggtgcaagac gcgcagaggt acggattttt catgaaacgc cgcaggacga tttacgaggg
     5461 taaggccaag attctttatg aggggccgga gccgggtacc cttatccaat atttcaagga
     5521 cgacaccacc gccttcgatg ccaagaagcg tgccgtgctc gaaggcaaag gcgtgctcaa
     5581 caatcgcatc tccgaatatc tcatgacgca actgaacggg atcggcgtgc cgactcattt
     5641 tgtgcgccgt ctcaacatgc gcgaacaatt gatcaaggaa gtggagatca tccccttgga
     5701 ggtggtggtc cgtaatgtcg ccgccggatc gctgtccaca cggcttggca tcgccgaagg
     5761 tcaggcgctg ccgcgctcca tcatcgaata ttattacaag aacgatgcgc tccacgatcc
     5821 atgggtgtcg gaggagcaca tcacagcgtt cgcctgggcg agcccgcagg atctggacga
     5881 catcgtgaac ctcaccatcc gcatcaatga tttcctgtcc gggttgtttc tgggcgttgg
     5941 catcaagctg gtcgatttca agatcgagtt cgggcggttg tgggaaaacg atttcatgcg
     6001 tgtcgttctg gccgatgaga tcagccctga ttcctgccgg ctgtgggaca tccagaccaa
     6061 tgaaaagctg gacaaggatc gcttccgccg cgatctcggc ggcgtcaccg aagcctattc
     6121 ggaagtggcg cggcggctcg gcatattgcc ggaatccacc cagggcggca cgggccctgt
     6181 gctcgtcaaa tgaaggcgcg tgtttttgta acgttgaaga gtggcgtgct cgatccgcaa
     6241 ggcaaagcca tcggccatgc cctgaacaat ctcggcttcg attcggtcgg cgacgtgcgg
     6301 caaggcaaag tcattgacat cgaattggcc gaaaaggatg agaccagagc gcgcgcgcgt
     6361 ctcaaggaga tgtgcgaaaa gctcctcgcc aataccgtga tcgaaaaata tgagatcgag
     6421 ttgaagccgt gaaatctgcc gtcattgtgt ttcccgcttc caattgcgac cgcgacgcgc
     6481 aaaccgcact cgccgatctg accggaacaa aaccgttcat ggtctggcac ggcgacacgg
     6541 aagtccccgc ttgcgacctg atcgtcctgc cgggcggatt ttcctatggc gattatctgc
     6601 gctgcggcgc catggcggcg cagtcgcaca tcatgcggga cgtgaagaag aaagctgaag
     6661 ccggcgttgc cgtgcttggc atctgcaacg gatttcagat cctgacggag accggccttc
     6721 tgccgggcgc tctgatgcga aacgccgatc tgaaattcgt ctgccgtccc gtgaatctgg
     6781 tggtcgaaga atcccaatcc gcgttcacgc gccattattc caaaggcgag caggtgcggt
     6841 tcccggtcgc gcatggcgaa ggcaattatt tcgccgatga cgggacgctc gaccgtctcg
     6901 aaggcgatgg acgtgtgctt ttccgctatg cgccgggtga caatcccaac ggatcggcgc
     6961 gcgatatcgc cggcatcttg aacgaaaagc gcaatgtgct gggcctgatg ccgcatcccg
     7021 agcgggcctg cgatcccctt ttgggttcga cgaacggccg cgcgctcttc caaagcctca
     7081 ttgaaacact gtcctaggtt ttggaatcgg tgacacagat tacgccacag atcgtccgcg
     7141 aacacggtct ttccgacagc gaatatgccc gcatcaagca gctcctgaat cgcgatccca
     7201 accttgtcga attgggcatc ttctcggtga tgtggagcga gcattgctcg tacaaatcca
     7261 cgcgcgtgca tttgcgcaaa ctcccgacca aagcgccctg ggtcctgcaa ggacccgggg
     7321 agaatgcggg cgtcatcgat atcggcgatg gtgacgcgtg catcttcaag atggaaagcc
     7381 acaatcaccc ctcctttatc gcgccctatc agggggctgc gacaggtgtc ggcggcatca
     7441 tgcgcgatgt cttcaccatg ggtgcacgcc ccattgccat gatgaacgca ctgcgcttcg
     7501 gcgagccggc ccatcccaag acccggcatc tgatttcggg cgtggtcgca gggattggcg
     7561 gttacggcaa ttgcatgggt gtgccgacag tgggcggcga ggtgaatttc caccgttcct
     7621 acaatggcaa catcctggtc aacgcgatgt gcgtcggcct tgcaaggagc gacaggattt
     7681 tcctttccgc cgccaagggc gcaggcaatc cagtcgtcta tatcggctcc aagacggggc
     7741 gcgacggcat tcacggcgcg accatggcat cggccgagtt caacgaagac tcagaagaaa
     7801 agcgccccac cgttcaggtc ggcgatccgt tcaccgagaa gctgctgctc gaagcctgtc
     7861 tcgaattgat ggcgaccgac gccatcatcg cgatccagga catgggcgcg gcggggctta
     7921 cctcgtcctc cgccgagatg ggggacaagg gcggatcggg catcgaactc gacctcgata
     7981 aggttccgca gcgcgaaacc aatatgaccg cctatgacat gatgttgtcg gaaagccagg
     8041 agcgcatgct cgcggtcctt aagccggggc gggaaggaga ggccgaggcg atcttcaaga
     8101 aatgggagtt ggatttcgca gtgatcggca tcaccacaga tactaaaaga ctcgtggtca
     8161 aacacaaggg cggggtcgtt gccgatatgc cgatcaccgc gctgtccgat gcggcgccgg
     8221 tctatgagcg tccccacgaa aagcgggcgc ccgcccgtag cgcacctatg ttcgacgcaa
     8281 cgcgccccgt gatggaatcg ctgatgacga ttctcgcttc gccggatatg tgttcgcggc
     8341 gctgggtgtg ggagcaatat gacaattcgg tcatggccga cacgattcag atgcccggcg
     8401 gcgatgcggc aattgtgcgc gtccatggca caaacaaggc gctcgccctc tcaaccgacg
     8461 tgacgccgcg ctattgcaga gccgatccgt ttgaaggcgc gaaacaggcg gtcgccgaat
     8521 gctggcgcaa tctgaccgcg gtgggggcgc tgccgctcgc gatcaccgac aatctcaatt
     8581 tcggcaatcc ggagcggccc gaaaccatgg gcgagatcgt tgcctcaatc gatggcatcg
     8641 gcgaagcgtc gcgaaaattg tccttccctg tcgtgtcggg aaattgctcg ctttacaacg
     8701 agacgaatgg cgagccgatc ctaccgacac ccgccatcgg gggcgtgggt ctcatcaaag
     8761 atctgtcccg catggcgagc atcgcgctca agcgtgaagg cgatgcgatc ctgctgatcg
     8821 gagaaaccaa gggccacatc ggccaatcgg tttacttgcg cgagatcgag ggccgcgagg
     8881 aagggcctgc gccttcgatc gatctggcgg cggaaaagag aaacggcgac ttcgtccgtg
     8941 gacagatcga acagggccgc gtcgacacgg tgcatgatct ttccgacggc ggcctgctgg
     9001 ttgcgcttgc ggaaatggcc ctggcgggtg gcattggcgt tgcgttggat ttggatacgg
     9061 acattgcact tctatttggc gaagatcagg gccgatacat tctttccgct ccggtcattg
     9121 cggcttcttc catcctggaa caggcaaagg cggcaggaat cgcggcgtct ttgctagggc
     9181 gcaccggggg cgataagatc attctggaag gtgccgacga aatttcgctt tcgaagattc
     9241 gctcggccca tgagggctgg tttccggcct atatgtcagg cggcgaactg ccgccaacga
     9301 actgagggtg cccacgatgg ccatgccggc caaggatatc gaactcgcca tccgcgccgc
     9361 tttgccggat gccgatgtca ccattaccga tcttgccggg gacggcgatc attacgcggc
     9421 catggtgaaa tccgccgcct tcaagggcaa aacccgcgtc cagcagcaca agatggttta
     9481 cgacgcgctc tcgggacgca tgggcgggca attgcacgcc ctctcgctgc agacttcagc
     9541 caaggattga acgcgcgcat cgccgtcttg acggaacgcg acgccgcccc tacctcaaag
     9601 agcgaaagga cacccccatg agccagactt ccgttcacga ccagatcgcc aaggacctcg
     9661 ccgaaaacga ggtgctgctt tacatgaagg gcacgcccgt attcccgcaa tgcggatttt
     9721 cggccgcgac cgtcggtgtg ctctcggagc tcggcgtcaa attcaaggcg gtggatgttc
     9781 tgaaagaccc cgaaatccgc gccggcatca aggaattctc caactggccg acgatcccac
     9841 aactctatgt gaagggtgaa ttcgtcggcg gctgcgacat cgtgaaagag atgttcgagg
     9901 agggcgagct tgaggcctac ttcaaggaaa aaggcatcgc gctccaaaat gcataaagaa
     9961 aaggccgcgt tttacgcgcg gcctttttgc actcgctctg agagacgatc agcgcgagta
    10021 atattccacc accaggcttg gctgcatctg caccgcatag ggcacatcgc caagcttggg
    10081 cgtgcgcagg aatttcgccg acatcttgcc atgatcggcc tcgatgtaat cgggcacttc
    10141 gcgttcgggg cttttcaccg cttcaagcac cagcgccata tccttcgcct tggagcgcaa
    10201 ctcgatgagg tcgccttcct tgacgagata ggacgggatg gtcacgcgct tgccattcac
    10261 cgagacatgg ccatggttga ccaattgccg ggccgaaaac ggcgtcggca cgaatttcgc
    10321 gcgatagacc accgcatcga ggcgacgctc caaaagcccg atcatcatct cgccggtgtc
    10381 gccggtcttg cgcgaggctt cctcgtaata gccgcggaac tgccgctcgg tgatgttggc
    10441 gtaatagccc ttgagcttct gcttggcgtg aagctgtgtg ccataatcgg aaagtttgcg
    10501 cgattttctc tgcccgtgct gcccggggcc atagttgcgt ttattaatgg ggctcttgcc
    10561 gcggccccag atgttctcgc ccatacggcg atcaagcttg tatttcgagg tcgtgcgctt
    10621 cgtcatgcgg tgttccggct cgcgtttaag gggtccggcg aggggtccga cgccgaaggc
    10681 cgcgcactat agccatggac ttttaactgt caaacgtcct tcggggggac aggctttggc
    10741 gggttgcggc gttttccctt ggtcagggca acaatgacac cccttaaggt ccgcacctcc
    10801 tgatccgtga gctgggcacg gtgcagaatg ctgcgcaaat tgcggatcat tccgggacgg
    10861 tttcccggag ggaacaggaa cccgctcttt tccagctcgc cttccagatg ttcgaacagg
    10921 cggaacagct ccgcgcgggg ggcaggcaat gcggcgccat ggtcgatctg ctcggtgggt
    10981 gtcgcatcgg cgctcctgaa ccattcgtag gccatcagga gcaccgcctg gcccagattg
    11041 atcgaggaaa aacttggatc ggtcggaatg gtgatcacac aatcggcgag cgccacctcg
    11101 tcattgctaa gccccgcgcg ctcgtttccg aacagcaggc cggtggggat gccggcaagg
    11161 gccgcctcac gcaggcggcg agcggcttcg gcgggggtga gcaccggctt tgccatggcg
    11221 cggtcgcgcg cggtcgatgc gtaaacaaga cgcagggacc caattgccgc cggaacgctg
    11281 tcatagacct gggccgccgc gatcaatccg gcagccccga ccgccatggc ttgcgccttc
    11341 tcattcggcc agccgtcgcg gggcttgacc aggcgcaaat ccgacagccc gaaattcgcc
    11401 atggcccggg cgcaagcccc gatattctcc cccaattgcg gctcagacag aataatgacg
    11461 ggtgcgagga ccagctcggg cattgcattc tcgattttgc cgcggcttcg actgaggcaa
    11521 ttggtggcgt gtaacagccc ctttgacggg aatagggttc aatcgctaag agacgcgggc
    11581 tttgctcccc atgtgaagga cgaacttatg gcgaaaatca aggtcgcaaa tcctgttgcc
    11641 gaactcgatg gcgacgagat gacccgcatc atctgggacc tcatcaagaa aaaactgatt
    11701 ctgccctatc tcgatatcac gctcgaatat tacgatttgg gcgtcgagca cagggacgcg
    11761 acaaacgatc aggtcaccgt cgacgcggcg gaggcgattc ggcgccttgg cgtcggcgtg
    11821 aaatgcgcca ccatcacgcc ggacgaagcg cgcgtgaagg agttcaacct caaacagatg
    11881 tggaagtcgc ccaatgggac gatccgcaac atcctcggcg gcacgatctt ccgcgaaccc
    11941 atcatctgca agaacgtgcc gcgcctcgtt ccgggatgga ctcagcccat cgtcatcggc
    12001 cgccatgcct atggcgatca atatcgctgc accgatttcc tggtgcccgg accgggcaag
    12061 ctgacggtcg cctggaccgg caaggacggc aagaagatcg agcgcgaagt gttcgatttc
    12121 ccgggcgcgg gtgtcgccat gaccatgtac aatctggatc aatcgatcat cgatttcgcc
    12181 cgcgcctcga tgaactatgc cttgatgcgg aaatatccgc tctatttcac caccaagaac
    12241 accatcgtca aagcctatga cgggcggttc aaggatctgt tcgagaaggt cttcaacgaa
    12301 gaattcacgg ccaaattcaa ggcggcgggt ctcacctacg aacatcggct gatcgacgac
    12361 atggtcgcgg cctgcctcaa atggtccggc ggctatgtct gggcctgcaa gaattacgac
    12421 ggcgacgtgc agtccgacac ggtggcgcaa ggctatggtt cgctcgggct gatgacgagc
    12481 gtgctgatga cgcccgatgg cgacacggtc gaatccgagg ccgcccacgg cacggtgacg
    12541 cgccattacc gccaacatca gaagggccag ccgacctcga ccaattcgat cgcctcgatc
    12601 ttcgcctggt cgcgcggact ttcccatcgc gccaagctcg acggcaattc cgatctgacg
    12661 catttcgcgt cgtcgctcga aaaagtctgc gtcgatacgg tggagtccgg cttcatgacg
    12721 aaggacctcg ctcttctcat cgggccggag gcgaaatcgc tttcgaccga gggcttcctc
    12781 gacaaggtcg acgagaacct gaagaaggcg atggcttagg cctttacgtc gtttccagcg
    12841 cggcgaggcg ttgctcgatg gcggagagcg cgtctttcgc gcgggccccg tcggggcctc
    12901 ccgcctgcgc catgtcgggc cgcccgccac cgcccttgcc gcccagagca tcggcacccg
    12961 cccgcaccag atcgacagcg ctgatgcgcg tggtgaggtc gggcgtgacg cccacgacca
    13021 gcgaagcctt gccgtcggcg accgtgacga aggccacgac gcccgagccg agtttggttt
    13081 tggcctcgtc cgcgagcgcc ttcaaatcct ttgccgcgac atcttcgacg atgcgcagca
    13141 cgtatttcac gccgccgatt tccttatagg cttcggcacc ggctttcgga cctcctgaca
    13201 gcgcaagcga tttcttggct tcggaaagct cgcgttccag ccggcggcga tcctcgacga
    13261 gagcatggat gcgcgagggc aattccacgg ctgaggtctt cagcacggcg gctgcttcgc
    13321 gcgcggcttc gccttgcgat tcgagataac gc
//
LOCUS       DASYEG010000211         6436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2239564, whole genome shotgun sequence.
ACCESSION   DASYEG010000211 DASYEG010000000
VERSION     DASYEG010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6436
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2239564"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..1322)
                     /locus_tag="VGM72_09490"
     CDS             complement(192..1322)
                     /locus_tag="VGM72_09490"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEY4265540.1"
                     /translation="MAAPTEAEIIKSLQTVRDSVTGKNVVEAGLIEGLATRGGHVTFA
                     VQVAPERGPAAEPLRKTCEDAVARLPGVLSVTAVLTAHRERNAKPRAPSPPAHGHAHN
                     HGPAPTDTSKGIAGVGSIVAVASGKGGVGKSTVAVNLALGLSALGLRVGLLDADIYGP
                     SVPRLLAITEKPRSDGKKLFPIEKFGLKAMSIGFLVDEDKPMIWRGPMVQSAITQMLG
                     DVEWSPLDVLVVDMPPGTGDAQLTLAQRVPLKGAIIVSTPQDLALIDARKGLNMFRKT
                     EVPVLGIVENMSLFICPNCGHESHIFGHGGARATARDLGCDFLGEIPLVPSIRETSDA
                     GTPIVAASPNSPEARAFVALAQIVADKLSTGTIRPAPKIIID"
     gene            1458..2588
                     /gene="hflK"
                     /locus_tag="VGM72_09495"
     CDS             1458..2588
                     /gene="hflK"
                     /locus_tag="VGM72_09495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEY4265541.1"
                     /translation="MPWNNQSGGGRPGGGGRGPWGNGPSGGGNTPPDLEALLKRSQDK
                     LRQLLPKGVGGGGTLIVALIVVAVWLASGIYVVDPDEQGVVTRFGAFVGRTTPGINYH
                     LPWPIEAVQTPAVTRENQLNIGYRIGNSEGARDVPEESLMLTGDENIVDINFTVFWVI
                     KDAGAFLFNVQNPDAQPDGTIKAVAESAMREVVGKNQIEPILTANREPIQDEVRDLMQ
                     RILDAYQAGVTVTRVQMQKADPPAQVIEAYRDVQAAQTDQDRMRNEAEAYANKVVPEA
                     RGQAARIVQQAEGYKQQVTAEAQGEAARFVSVYDQYKKAPDVTRRRIYLDTMSEVLGG
                     MNKVIVDNKGGSGVLPYLPLPELKQGTPRSGAPVADARGGRP"
     gene            2585..3466
                     /gene="hflC"
                     /locus_tag="VGM72_09500"
     CDS             2585..3466
                     /gene="hflC"
                     /locus_tag="VGM72_09500"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEY4265542.1"
                     /translation="MKRHHTIAIVAAGVVLLIVLMSSLFTVPQTEQALVLQVGEPRAV
                     VTEPGLHMKLPWPFQNVLYLDKRVLFLDLPAQEVIAQDKKRLVVDAFARYRITDPLRF
                     YQSLTDETVAQVRLQPILGSNVRRILGSQTFQAVLSGERAKLMIDIREGVNAQTKNFG
                     IEVVDVRIRRADLPQQNSDAIYRRMQQERVRQANEFRAQGEQISQEIRSKADRDATVT
                     VAEATRQGEITKGQGDAEKNRIFAEAFGQDPDFFSFYRSMTSYEQALKGDNTTVILSP
                     DSDFFRYFSQGAGAARR"
     gene            3724..5313
                     /locus_tag="VGM72_09505"
     CDS             3724..5313
                     /locus_tag="VGM72_09505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEY4265543.1"
                     /translation="MPMASVSRSGARPRFMTFASTLVLASFGLAAGTLAAPARPAAPV
                     TKAAAVPAIPARPAAAAPAIAVPPSIAARPQGYADLAERLLPMVVNISTSQTLRRRPD
                     AGADAAPAPQAPQGSPLDDFFKDFLDRGNRPRRVQSLGSGFVIDPAGYIVTNNHVIEG
                     ADEITVILSDTTSMPATLVGTDEKMDLALLKVNSKQPLAVAKLGDSDKARVGDLVMAI
                     GDPFGLGGTVTTGIVSARNRDIKSGDYDDFIQTDAPINKGNSGGPLFNMDGEVIGINT
                     AIYSETGGSVGIGFSIPSNAAKNIIAQLKTTGKIQRGWLGVRIQQVSPDIAETIGLDK
                     ARGALIAGMTTGGPAAKAGIQNGDVVLTFDGKPVADSRALPRLVADASIGKTVNIEVL
                     RKGQHKSIPISIQKLPDEGKPSAVSKTPAKPGATAPKPPATVNLGMNLAPLSAESRRR
                     FHLEGNVQGVVVTDVDADSPAGQKNIRAGDVITEVAQQKVSSPDDVSARLDAERKAGH
                     KVVLLQVSRGGELTFIGVRLP"
     gene            complement(5325..5801)
                     /locus_tag="VGM72_09510"
     CDS             complement(5325..5801)
                     /locus_tag="VGM72_09510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4265544.1"
                     /translation="MSAAFLVRPWREADTEELGELFRKSVREIAGRDYRPAQIEAWVK
                     APGAIHTWTERMQERVIFVAEQRQSLIGFIQYEPPDHIDMTYVHPQSQRIGVASALLA
                     AVETDARRRGVMLLNVEASITSRPFFQARGFEIITPQIVTALGEDFLNYRMSKQLT"
     gene            complement(5798..>6436)
                     /gene="serB"
                     /locus_tag="VGM72_09515"
     CDS             complement(5798..>6436)
                     /gene="serB"
                     /locus_tag="VGM72_09515"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEY4265545.1"
                     /translation="MDSTIIGCECIDELADIAGIKPKVAAITERAMRGELDFPSALRM
                     RVALLTGLPLEALARVYAERVRLNKGARTLTATMRKNGAHTLLVSGGFTYFTARVAAD
                     AGFEDNQGNRLLDDGKRLTGAVGEPILGREAKLRALENAVAHYGFSFAETLAVGDGAN
                     DLDMIARAGMGVAYHAKPVVAAAADSAIAHGDLTSLLYLQGYRANEIVAATA"
BASE COUNT         1194 a         2060 c         2012 g         1170 t
ORIGIN      
        1 cgcttggcgg gagcggcggc ctttacccgt ccgcgtttcg ccgcacctgt cttggccttg
       61 cggccgcgcg tcatcttttg tctttcgtcc attccctcgg cttgacgttt tgcgccacgc
      121 ggcatggtca tctcctcaga atcgccccat gggaacgaaa cacctgaagc gtgacggtag
      181 ttccagaaaa tttagtcgat gatgattttt ggcgcgggac gaatcgtccc ggtcgataat
      241 ttgtccgcca cgatttgcgc gagcgcaacg aacgcgcgcg cttcgggact gttgggcgat
      301 gccgccacga tgggcgtacc ggcatcggaa gtttcgcgga tcgacggcac cagcggaatc
      361 tcgccgagaa aatcgcaacc cagatcgcgc gccgtcgcgc gtgcgccgcc atgaccgaag
      421 atatggctct catgcccgca attgggacag atgaagagac tcatgttctc gacgatgccg
      481 agcaccggca cttcggtctt gcggaacatg ttgagaccct tgcgcgcgtc gatcagcgcg
      541 agatcctgcg gcgtcgagac gatgatcgcg cccttcagcg gcacgcgctg ggcgagcgtc
      601 aattgggcgt cgccggtgcc cggcggcatg tcgaccacga gcacgtcgag cggcgaccat
      661 tccacatcgc ccagcatctg ggtgatcgcg ctctgcacca tgggcccgcg ccagatcatc
      721 ggcttgtcct catcgaccag gaagccgatg gacatggctt tcagtccgaa tttttcgatg
      781 gggaatagct tcttgccgtc ggagcggggt ttttccgtga tggcgagaag acgcggcacc
      841 gatggaccgt agatgtcggc gtcgagcagg ccgaccctga gccccagcgc cgaaagaccc
      901 agcgccaggt tcaccgcgac cgtcgatttg cccacgcccc ccttgcccga ggccaccgcg
      961 acgatggagc caacgccggc gatgcccttg ctggtgtcgg tgggggcggg gccgtgattg
     1021 tgcgcgtgac cgtgcgccgg ggggctggga gcccgcggtt tcgcattgcg ctcgcgatga
     1081 gcggtgagga cggccgtcac cgacagcacg ccgggcaggc gcgccaccgc atcttcgcat
     1141 gtcttgcgca gcggttcggc ggcgggtccg cgctcgggcg ccacctgaac cgcgaatgtc
     1201 acatggccgc cgcgtgtggc caaaccttcg atcaggccgg cctcgaccac gttctttccg
     1261 gtcacggaat cgcgaactgt ttgtaaactc ttgatgattt cggcctctgt gggggcggcc
     1321 atgggtctct cttgcggtgg ctatatttgc gaagacgaag ataagaccat atataggccg
     1381 cgccgggcga aaccaatcat tggtcggctt gccttgaatt tgggcacggt ctatcgcaac
     1441 tcggctgagg gaatccaatg ccttggaaca atcagtccgg cggcggcagg cccggcggag
     1501 gtggacgcgg cccctggggc aacggtcctt ccggcggcgg caacactccg cccgatcttg
     1561 aagcgctcct caaacgcagc caggacaaac tgcgccagct cttgcccaag ggagttggcg
     1621 gcggcggcac tctgatcgtc gcattgatcg tggtggcggt ctggctggca tccggcatct
     1681 atgtggtcga tcccgacgag cagggtgtgg tcacgcgttt tggcgccttt gtcggacgca
     1741 cgacgccggg catcaactac catctgccct ggcccatcga ggcggtgcag acacctgcgg
     1801 tcacgcgcga aaaccagctc aacatcggtt atcggatcgg caattccgaa ggcgcgcgcg
     1861 atgtgccgga agaaagcctg atgctgaccg gcgacgagaa catcgtcgac atcaatttca
     1921 cggttttctg ggtcatcaag gatgcgggcg cgttcctgtt caacgtacaa aatcccgacg
     1981 cccagccgga cgggacgatc aaggcggttg ccgaaagcgc gatgcgcgaa gtggtcggca
     2041 agaaccagat cgagcccatc ctcaccgcca atcgcgaacc catccaggat gaagtgcgcg
     2101 atctgatgca gcgcatcctc gacgcctatc aggcgggagt cacggtgacg cgggtgcaga
     2161 tgcagaaagc cgatccgccg gcccaggtca tcgaggccta tcgcgacgtg caggccgctc
     2221 aaaccgatca ggaccggatg cgcaacgagg ccgaagccta tgccaataag gtcgtgcccg
     2281 aagcacgcgg ccaggccgcg cgtatcgtcc agcaggccga aggctacaag cagcaggtca
     2341 cggccgaagc gcagggcgag gccgcccgct tcgtctcggt ctacgatcag tacaaaaagg
     2401 cgcccgacgt cacgcgccgc cgcatctatc tcgatacgat gagcgaggtg ctcggcggga
     2461 tgaacaaggt aatcgtcgac aacaagggtg gctcgggcgt gctgccctat ctgcccctgc
     2521 cggaactgaa gcagggaacg ccgcggtccg gcgcgcctgt cgccgatgcc agaggagggc
     2581 ggccatgaag cgccatcaca ccatcgccat cgtcgcggcc ggcgtcgttc tgctgatcgt
     2641 gctgatgtcg tcccttttta ccgtgcctca aacggagcag gcgctggtgc tccaggtcgg
     2701 cgagccgcgc gcggtcgtca ccgaacctgg tctgcatatg aaactgccct ggccgttcca
     2761 gaacgtgctc tatctcgaca agcgcgtgct gtttctcgat ctgccggcgc aggaagtcat
     2821 cgcgcaggac aagaaacgcc tggtggtgga cgcgttcgcg cgctaccgca tcaccgatcc
     2881 tttgcgcttc tatcagtcgc tgaccgatga gaccgtggcg caagtccgcc tgcaacccat
     2941 cctcggctcc aatgtgcgcc gcatcctggg ctcgcagacc ttccaggcgg tgctctccgg
     3001 tgaacgcgcc aagctgatga tcgacatccg cgaaggcgtg aatgcgcaga caaagaattt
     3061 cggcatcgaa gtggtcgatg tccgtatccg tcgcgccgat ctgccgcagc agaacagcga
     3121 cgccatctat cgccgcatgc agcaggaacg cgtgcggcag gccaatgagt tccgcgctca
     3181 gggtgaacag atttcccaag aaatccgcag caaggcggat cgcgacgcga ccgtcaccgt
     3241 ggccgaagcc acccgtcagg gcgagatcac caagggacag ggtgatgcgg agaagaatcg
     3301 aatcttcgcc gaggcgttcg gccaggatcc ggatttcttt tcgttctacc gctcgatgac
     3361 atcctatgag caagcgctca aaggcgataa caccacggtc atcctgtcgc ccgacagcga
     3421 tttcttccgc tatttcagcc agggtgccgg tgccgcaagg cgctgatcga aaaacgaaac
     3481 cctgtgcaac gcgagtgccg aaccggccaa ggcccggttt cggcattcgc acgcttggcc
     3541 gacttgccgg gaaccccgtc gcagagcatg atggatgagg atgaccgttt gaggaatgct
     3601 caagggaaaa tgaacgtcct ttccgctggg tttccttaaa ctgcggctac agtgatgcgt
     3661 cattcgtttc ttgttcgggg ccacaagctc caggtttgtc gtttcggcat agaggatcgt
     3721 tccatgccta tggcttccgt ctcccgctcc ggcgcgcggc cgcgttttat gacctttgcc
     3781 tccacgcttg ttctggcctc gttcggtctt gcggccggca cgttggcggc gcccgcaagg
     3841 cccgccgctc cggtcacaaa agcggcagcg gtaccggcga ttccggcgcg gcccgccgcc
     3901 gcggcgcccg cgatcgcggt tcctccctcc atcgcggcgc gtccgcaagg ctacgccgat
     3961 ctcgccgaac gcctgctgcc gatggtcgtc aatatctcga cctcccagac gctgcgccgc
     4021 cgtcccgatg ccggcgcgga cgcggccccc gctccgcagg ccccgcaagg ttcgccgttg
     4081 gacgatttct tcaaggattt cctggatcgc ggcaaccggc cgcgccgcgt ccagtcgctg
     4141 ggctcgggct tcgtcatcga tccggccggt tatatcgtga ccaacaacca cgtcatcgaa
     4201 ggcgcggacg aaatcacggt catcctgagc gacacgacat cgatgccggc gacgctcgtc
     4261 ggcaccgatg agaaaatgga tctcgcgctc ctgaaggtga attcgaaaca accgctcgcg
     4321 gtcgccaaac tcggcgattc cgacaaggcg cgcgtcggcg atctggtgat ggccatcggc
     4381 gatccgttcg gccttggcgg cacggtgacc accggtatcg tgtcggcccg caaccgcgac
     4441 atcaaatccg gcgattatga cgatttcatc cagaccgacg ccccaatcaa caaaggcaat
     4501 tccggcggtc cgctgttcaa catggatggc gaagtcatcg gcatcaatac ggcgatttat
     4561 tctgagaccg gcggatcggt cggcatcggc ttctcgattc cgtccaacgc cgcgaagaac
     4621 atcatcgcgc aattgaagac gaccgggaaa attcagcgcg gctggctcgg cgtgcgcatc
     4681 cagcaagtct ctcccgatat tgccgagacg atagggctgg acaaggcccg cggcgcgctg
     4741 atcgcgggca tgaccaccgg cgggcctgcg gccaaagccg gaatccagaa cggcgacgtg
     4801 gtgctgacct tcgacggcaa gccggtcgcc gattcgcgcg cgttgccgcg cctggttgcc
     4861 gatgcctcca tcggcaagac ggtgaatatt gaagtgctgc gcaaaggcca gcacaagagc
     4921 ataccgattt cgattcagaa gcttcccgac gagggcaagc cgagcgcggt cagcaagact
     4981 ccggccaaac ccggagccac tgcgccgaag ccgcccgcaa ccgtcaatct cggcatgaat
     5041 ctggcgccgc tttcggccga atcgcgccgc cgtttccatc tcgagggcaa cgtccaaggc
     5101 gtcgtcgtca ccgatgtgga tgccgacagt ccggcgggtc agaagaacat ccgggcgggc
     5161 gatgtcatca ccgaagtggc ccaacagaaa gtgtcgtcgc ccgacgatgt gtccgcgcgg
     5221 ctcgatgcgg agagaaaggc cggccacaag gtcgtgctgc tccaggtcag ccgcggcggt
     5281 gaactcacct tcatcggcgt acgcctgccg taacgccgcc gggtttaagt gagctgtttc
     5341 gacatccggt aattcaggaa atcctcgccg agggcggtga cgatctgcgg cgtgatgatc
     5401 tcgaatccgc gtgcctggaa aaagggccgc gaggtgatgc tcgcctccac attgagaagc
     5461 atgacgccgc gccgccgcgc gtcggtctcg accgccgcca gcaacgcgct ggccacgccg
     5521 atgcgctggc tttgcggatg cacataggtc atgtcgatat ggtccggcgg ctcatattgg
     5581 ataaacccaa tcaggctctg gcgttgttcg gcgacgaaaa tcacgcgctc ttgcatgcgt
     5641 tcggtccagg tgtgaatcgc gcccggcgcc ttgacccagg cttctatctg ggccgggcga
     5701 taatcgcggc ccgcgatttc gcgcacgctt ttacggaaca gttcgcccaa ttcttccgtg
     5761 tccgcttccc gccagggccg cacgaggaac gcggcgctca tgcggttgcg gctacgattt
     5821 cattggcgcg atagccttgc aggtagagca gcgatgtcag atcgccatgc gcgattgcgc
     5881 tgtctgccgc tgccgcgacg acgggcttcg catgataagc gacacccatc ccggcacgcg
     5941 cgatcatgtc gagatcattc gctccatcgc cgacggcaag cgtctcggcg aaggaaaagc
     6001 cataatgcgc gacggcattt tcgagcgcgc gaagtttggc ctcgcgtccg aggatcggct
     6061 cgccgaccgc gcccgtcagc cgcttgccgt cgtcgagaag ccggttgccc tgattgtcct
     6121 cgaagccggc atcggccgcc acgcgcgccg tgaaataggt gaagccgccc gataccagca
     6181 gcgtgtgcgc gccgttcttg cgcatcgtcg cggtgagcgt gcgcgcgccc ttgttgagcc
     6241 gcacgcgttc ggcatagacg cgcgccaatg cttccagcgg caaaccggtg agcaacgcga
     6301 cgcgcatccg caaggcgctg ggaaaatcga gctcgccgcg catggcgcgt tcggtgatgg
     6361 ccgcgacctt cggcttgatg cccgcaatgt ccgccagctc gtcgatgcat tcgcagccga
     6421 tgattgtgga atccat
//
LOCUS       DASYEG010000212         4612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4707314, whole genome shotgun sequence.
ACCESSION   DASYEG010000212 DASYEG010000000
VERSION     DASYEG010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4707314"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VGM72_09520"
     CDS             complement(<1..477)
                     /locus_tag="VGM72_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265546.1"
                     /translation="MDRFSPVRAGLVLAIVMAVVQACGVSGQGADGARPKNVILMIAD
                     GSGASAIAADGLYTGRLGRQIFDRTDWIKAWSSTYSLRLGAKPIEGPDGLKQEPDAAY
                     DPAKAWDTMPVTTKTGAFEDRFAGYGWTKKTAPDSADTMSAIVTGRKTYDGAINVDG"
     gene            697..1425
                     /locus_tag="VGM72_09525"
     CDS             697..1425
                     /locus_tag="VGM72_09525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEY4265547.1"
                     /translation="MAEVKLPRRDFLKAAGAAGAAGAAAATGLASCSPEAKTSAAASA
                     PGPQNNGPETWQVLTPQEVAFFSAAADTIIPKDDLSPSGTECGVPVFVDRQLASAWGG
                     GAKMYRAGPFLKGMPEQGYQLSLTPHEFFVAGVRATNDWCVKTYGKTFDRLEQDKRNE
                     TFTAMQGGKAEFDGLDAKQFFGQLLTINMEGFFADPIYGGNRNKASWRMLGFPGLPAT
                     YADKIDEYRNKRYVAEPQSIADFS"
     gene            1438..3207
                     /locus_tag="VGM72_09530"
     CDS             1438..3207
                     /locus_tag="VGM72_09530"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY4265548.1"
                     /translation="MVTKLKEVDAVVIGQGLTGVMMAKELTMAGLTVVGLERGADRKP
                     EEEVVLPRIRDELKYNARKELMIDNSIDTVSFRNASDQMALPIRRWGAFLPGEGVGGT
                     TNHWGGLHWRFLPTDYRIKSAVTERYGAKMIPADMTIQDWPVTYDELEPYYDKFDKLC
                     GVSGKAGNLKGQKIEGGNIFEGPRANEYPNPPLKTSAGALLLADACKKVGYHPFPVPF
                     SAPSRPYTNIYGVTLGSCEYCGFCGRTSCEANAKATSATNVMPVLRREPKYTLKTRAF
                     VSKILYDKQAKKVTGVTYTDMKTGEEYEQPAGMVILSAFVFGNTHMMLYSGIGEPYDS
                     KTGKGLVGKNLCYQFEAGGTAFFEDKNLNPFMGSPGDRVAFDDFNGENFDHSGLGFLG
                     GGYITGGSGSAPPIGGRDVPEGTPSWGSEWKKQTVKWYYGSADFNTQGSVYANRANYL
                     DLDPTYKDSIGRPLLRMTYNGTDNDHKMSAYLLSKLEVAIKAMNPSSYELRHRPKNFT
                     VVPYQSTHNTGGTMMGTNPSNSVVNRFQQAWEASNLFILGASTFPQQHGYNPTGPLGA
                     LAYWTADKIVTDYIKKPGPLVQA"
     gene            3228..3590
                     /locus_tag="VGM72_09535"
     CDS             3228..3590
                     /locus_tag="VGM72_09535"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY4265549.1"
                     /translation="MRLSLSGAAIGALLLAGAPASAADANHGKTLFVACQACHTEKPD
                     ALGPSLKGIVGRKSASLEDFRYSAAMKRANLTWDEKNLSDYLKDPQAKVKGNRMPFSG
                     FGNDNDTADVVAYLQTLK"
     gene            complement(3600..4232)
                     /locus_tag="VGM72_09540"
     CDS             complement(3600..4232)
                     /locus_tag="VGM72_09540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HEY4265550.1"
                     /translation="MFALNHELSLVFATPLSLRTVPNAATLNAGLERAILSRRQTYGG
                     NRISNIGGWQSLPDLLDWPEPEVKQLAAEIDRTVQQMWTLPAALERRPRESQTRAVYR
                     AYGWANVNEPGHYNMIHMHPGNHFSAVYYVATGKPAPDHEMNGRLELRDPRPAATYCR
                     MPGSTSESLLIRPQPGMMVVFPAWIEHWVHPFQGDGQRISIAVNITFDGH"
     gene            complement(4430..>4612)
                     /locus_tag="VGM72_09545"
     CDS             complement(4430..>4612)
                     /locus_tag="VGM72_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005883995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY4265551.1"
                     /translation="TLYPGDIIATGTPAGVGPMRPGDIVKIAFERVGEMTLDVVQGKS
                     GQRSIFAQAVPADAKT"
BASE COUNT          936 a         1468 c         1384 g          824 t
ORIGIN      
        1 gccatccaca ttgatggcgc cgtcataggt tttgcggccc gtaacgatgg cgctcatcgt
       61 atcggcggaa tcgggagcgg ttttcttggt ccagccatag cccgcgaaac gatcctcgaa
      121 ggcgccggtc ttggtcgtca ccggcatggt gtcccaggct ttcgcgggat cgtacgccgc
      181 atccggctcc tgcttcaggc cgtccggacc ttcgatgggc ttggcgccaa ggcgcaacga
      241 ataggtcgag gaccacgcct tgatccagtc ggtacgatcg aaaatctgcc ggcccaatcg
      301 ccccgtatac aggccgtccg cggcgatggc gctcgcaccc gatccgtcgg cgatcatcag
      361 gatgacgttc ttgggccgcg cgccatcggc gccctgcccc gatacgccgc acgcctggac
      421 cacggccatg acaatggcca gcaccagccc tgcgcggaca ggcgaaaagc gatccatgcg
      481 accctccata ttgcggcgac tcattatgca gcaattctat ggcacaccga taacatggcg
      541 cccgtgtccg ccgggaaaac cgaggaaaac ccgccccaat cattctctcg cccgccaatg
      601 ggtttcggac taatctcccg ccccagcaaa tgtcccgcga ggctttcgcg aggtagcatc
      661 ggccggacgg cgatccggcc gtcaagggag gcacacatgg ccgaagtcaa acttccacga
      721 cgcgatttcc tcaaagccgc cggggccgcg ggcgcggccg gcgcggccgc cgcaaccggg
      781 cttgcgtcct gcagccccga ggcgaagacc agcgcggcgg caagcgcccc cggtccacag
      841 aataacggcc cggaaacctg gcaggtcctg accccgcaag aggtcgcgtt cttttccgcc
      901 gcggccgaca ccatcattcc gaaagacgat ctgtcgccat ccggtaccga atgcggcgtg
      961 cccgtcttcg tcgaccgtca gctcgcgagc gcctggggtg gcggcgccaa gatgtatcgc
     1021 gcgggtccct tcctcaaggg catgcccgag caaggctatc agctctcgct gacaccgcac
     1081 gaatttttcg tcgcgggcgt cagagccacc aatgattggt gcgtgaagac ctatggcaag
     1141 accttcgacc ggctggagca ggacaagcgc aacgaaacct tcaccgccat gcaagggggc
     1201 aaggcggagt tcgacgggct ggatgcgaag caattcttcg gacagcttct caccatcaac
     1261 atggaagggt tcttcgccga tcccatctat ggcggaaacc gcaacaaggc gtcgtggcgg
     1321 atgctcggct tcccggggtt gcccgcgacc tatgccgaca agatcgacga atatcgcaac
     1381 aagcgctatg tcgccgaacc gcaatcgatt gcagattttt cgtgagggga tgaaccaatg
     1441 gtcacgaaac tgaaggaagt cgacgcggtc gtcatcggcc aaggcctcac cggcgtcatg
     1501 atggcaaagg aattgacgat ggccggcctt acggtcgtgg gcctcgaacg gggcgccgac
     1561 cgcaaaccgg aagaagaggt cgtgctgccg cgcatccgcg acgagctgaa atacaatgct
     1621 cgcaaggagc tgatgatcga caattcgatc gacaccgtca gcttccgcaa cgcctcggat
     1681 cagatggcgc ttcccattcg gcgctggggc gcgttcctgc ccggtgaagg cgtgggcggc
     1741 accaccaatc attggggcgg cctgcattgg cgattcctgc cgaccgatta ccgcatcaaa
     1801 agcgcggtga cggagcgtta tggcgccaag atgattcccg ccgacatgac catccaggat
     1861 tggccggtca cctatgacga gctcgaaccc tactacgaca agttcgacaa gctctgcggt
     1921 gtatcgggca aagccgggaa tttgaaaggc cagaagatcg agggcggcaa tattttcgaa
     1981 ggcccgcgcg ccaacgaata tcccaacccg ccgctgaaga cgtcggcggg cgccttgctg
     2041 cttgccgatg cctgcaagaa ggtgggctac catccctttc cggttccgtt ctcggcgcca
     2101 tcgcggccct acaccaacat ctatggcgtg acactgggct catgcgaata ttgcggcttc
     2161 tgcgggcgca cgtcgtgcga ggcgaacgcc aaagcgacat cggccaccaa cgtcatgccg
     2221 gtgctgcgcc gcgagcccaa atacaccctc aagacgcgcg ccttcgtgtc gaagatcctc
     2281 tacgacaagc aggccaaaaa agtcaccggc gtcacctaca ccgacatgaa gaccggcgaa
     2341 gaatatgagc agccggccgg catggtgatc ctttcggcct tcgtcttcgg caacacccac
     2401 atgatgctct attcgggcat tggcgaaccc tacgattcca agaccggcaa gggtctggtc
     2461 ggcaagaacc tttgctacca gttcgaggcc ggaggcaccg ccttcttcga ggacaagaac
     2521 ctcaatccgt tcatgggctc gccgggcgac cgagtcgcct tcgatgattt caacggcgag
     2581 aatttcgacc attcgggatt gggcttcctc gggggcggct atatcacggg cggcagcggc
     2641 tccgcgccgc cgatcggcgg ccgcgacgtg cccgagggca cgccgtcctg gggctcggaa
     2701 tggaagaagc agacggtcaa atggtactac ggcagcgccg atttcaacac gcaaggctcg
     2761 gtctacgcca atcgcgcgaa ctatctcgat ctggatccga cctataagga ctccatcggg
     2821 cggccgcttc tgcgcatgac ctataacggc accgacaacg accataagat gtcggcctat
     2881 ctgctgtcga agctcgaagt cgcgatcaag gcgatgaacc cgtcgagcta tgaattgcgc
     2941 caccggccga agaacttcac cgtcgtgcct tatcaatcga cccacaatac gggcggcacg
     3001 atgatgggga ccaatccgtc gaacagcgtg gtcaaccgct ttcagcaggc gtgggaggcg
     3061 agcaatctgt tcatcctggg ggcatccacc ttcccgcagc agcatggcta caatccgacc
     3121 ggaccgctcg gcgcgcttgc ctattggacg gcggacaaga tcgtgacgga ctacatcaag
     3181 aagcccggcc cgctggtgca ggcataaaaa ttcagggaga catattcatg cgtttgtcgt
     3241 tgtcgggcgc cgccatcggc gccctccttt tggcgggtgc gcccgcttcc gccgccgatg
     3301 ccaatcacgg caagaccctg ttcgtggcgt gccaggcctg ccacacggaa aaacccgatg
     3361 cgctgggccc gagcctgaag ggaatcgtcg ggcgcaaatc ggcatcgctg gaggatttcc
     3421 gctattccgc agcgatgaag cgcgccaatc tcacctggga cgagaaaaac ctcagcgact
     3481 atctgaaaga cccgcaagcc aaggtgaagg gcaatcgcat gccgttctcc ggctttggca
     3541 atgacaacga caccgccgat gtggtcgcct atctgcagac gctgaaataa gccgcgcctt
     3601 cagtggccgt cgaacgtgat gttgacggcg atggaaatgc gctgaccatc gccctggaac
     3661 ggatgcaccc aatgctcgat ccaggccggg aataccacca tcatgcccgg ctgcggccgg
     3721 atcaatagcg actcgctcgt cgaacccggc atgcggcaat aggttgccgc ggggcgcggg
     3781 tcgcgcagtt cgaggcgacc gttcatttcg tgatcgggcg caggctttcc cgtcgccacg
     3841 taatacacgg cggagaaatg attgcccgga tgcatgtgga tcatgttgta atggccgggc
     3901 tcattcacat tggcccagcc ataggcccga tagacggccc gcgtctggct ttccctcgga
     3961 cggcgctcaa gcgccgccgg cagggtccac atctgctgta ccgtgcgatc gatttcggca
     4021 gccagttgct tcacttccgg ctccggccaa tcgagcagat cgggtagcga ctgccagccg
     4081 ccgatattgc tgatgcggtt tccgccatag gtttggcggc gggaaaggat ggcgcgctcc
     4141 aatccggcat tgagcgtggc cgcgttcggc actgtgcgca gcgaaagggg tgtcgcgaag
     4201 acaagcgaaa gttcgtgatt cagcgcgaac attggcccac gcccccctcg ggatcggcgg
     4261 tctatcccaa gcccgtctcc aggcctccgc cgaaccgcac gattatgaca aaaatttcat
     4321 aattttgtat caggccccgc cctacggcgt gggcgatcaa gcgcttttga tcgcgagaaa
     4381 cgactggggt tttgcggtag gcgttttgcg aatgcttccc gctcgcgcgt caggtcttcg
     4441 cgtccgccgg gaccgcctgg gcgaaaatcg agcgttggcc gctcttgcct tgcaccacgt
     4501 cgagggtcat ctcgccgacg cgctcgaacg cgattttcac aatgtcgccg ggccgcatcg
     4561 gccccacacc ggcgggcgtt cccgtcgcga tgatgtcgcc gggataaagc gt
//
LOCUS       DASYEG010000213         6029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2386150, whole genome shotgun sequence.
ACCESSION   DASYEG010000213 DASYEG010000000
VERSION     DASYEG010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2386150"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1565)
                     /locus_tag="VGM72_09550"
     CDS             complement(<1..1565)
                     /locus_tag="VGM72_09550"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4265552.1"
                     /translation="MNDSVLQNAMRAHQTGNLSEAARLYGEVLRGDPRHFQALFALGA
                     VYYQAGRFEEAQRLIANAIAINPRVPEAFFTRGCAFQRLNRAPEALACFDQALALKPN
                     LADAHTNRGMCLMALNRDEQALESLDLALALDPANVGTWNNRGCVLHKMGRYDDAIAA
                     FGKALVSVPRSVEALVNRGSAYGALKRYGEAANDFQRVLVIDPEFAYARGNLILNRMQ
                     ASDWRHFEADRNAISSGLAAGKRIIYPFINVALSRSPADQLQCARLWVAHQAPLSPAP
                     LWRGEIYRHEKIRVAYISADFHAHATPALMAGVFEQHDRGRFELIALSFGPDDKSEMR
                     TRVKAAFDRFIDVREKSDAETALLMRQMEIDIAVDLKGYTKDHRSGIFAHRPAPIQAS
                     YLGYPGTMGAPYFDYIIADAIVIPDDQRPYYTEKIVHLPDSYQCNDSRRAIAGHRFTR
                     REVGLPDSGFVFCCFNNNYKITPEIFALWMRLLHGVPVSVLWLLEDNTEAMRNLKREA
                     EAHGIAANRLVFAP"
     gene            complement(1615..3366)
                     /gene="ggt"
                     /locus_tag="VGM72_09555"
     CDS             complement(1615..3366)
                     /gene="ggt"
                     /locus_tag="VGM72_09555"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY4265553.1"
                     /translation="MMKRVASAFARLSRTFTIAGTIAGWPILAVFASQIPPAQAAAPA
                     QHHMIVAAEPDAAEAGLAMLRNGGSAVDAAIAAQMVLTLEEPQSSGIGGGAYLIVADG
                     TAIHAYDGRETAPASARPDMFLDKDGKPRRHNDAVPGGLSVGVPGAVRMLAMAHAAHG
                     RLAWAKLFEPAIKLADEGFKVPPRLAIELAQGGPRLAAMPAIKAQFLHPDGTPIKAGE
                     TWRNPMLAQTFRKIAQNGPDALYTGAIASEITAAVANAPVNPATMTLADLAGYRAKER
                     TPLCGTYRAYRVCSLPPSTSGGVTVLQILGLLQHFPSSQLQPSTLSAVHLVSEASRLA
                     YADRAQWLGDTDFVPVPLAGLLDPAYLDSRARLIDPMRSMGVAPAGRPLMQKTELPDY
                     APMRPQIEMGTSHLAVVDDRGEALSMTTSVEAGFGAQIAAGGFLLNNQLTDFSFEPVI
                     DGKPVANAPAPGKRPLSAMSPTLVFAPDGQFFAAVGSPGGRQIIAYVAEALVNLIDAK
                     LSMPDVAAAPRHVNMNGATLIERGTALDQLTPALTAMGHQVRVIAFDSGVNGIRRVSG
                     GYEGGADPRREGVALGD"
     gene            3639..3715
                     /locus_tag="VGM72_09560"
     tRNA            3639..3715
                     /locus_tag="VGM72_09560"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3673..3675,aa:Arg,seq:acg)
     gene            complement(4165..5520)
                     /locus_tag="VGM72_09565"
     CDS             complement(4165..5520)
                     /locus_tag="VGM72_09565"
                     /inference="COORDINATES: protein motif:HMM:NF019149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEY4265554.1"
                     /translation="MGREKPPKGVNIASDFATQSFNTTTSMGRLTLNVLLSFAQFERE
                     VTGERIRDKFAASKKKGMWMGGYPPLGYDIDSRKLVVNPPEAETVNLIFKRYLDLGCV
                     KALHGDLAAKGVVSKRWTASTGVAHSGEPLGRGALYCLLKNRVYIGKTMHKGVSYPGE
                     HQAIITQDLFDAVQARITGYRKREPGKARSSQDAMLTGILFDAEGTLMTPTYSRKPDG
                     RRYRYYTSQSRLKGNHSNVSISRVPAAALEDLIKSALTRLQLSGSPDACQDDRSVLSR
                     ITIKSNSVTLEFDREAAVQKWRATNTEAQRATAQVLVDDCRERLASGEELTDGEHLIL
                     TLPVRARFRGGRASILDPAGAGSRPSLPDASLIKALARAHSWKRMLIEGEVGSVDALA
                     VKLGQERRHVGRTLNLAFLSPALTRSILSGEQSPNVRLARLLDAVIPLSWRAQQSFLG
                     P"
     gene            5593..>6029
                     /locus_tag="VGM72_09570"
     CDS             5593..>6029
                     /locus_tag="VGM72_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEY4265555.1"
                     /translation="MIGVDKIGEIRRAYFEQHRPIKEIVRTLSVSRATVRKVIRGHRT
                     EFKYERGIQPTPKLGDWVEVLTEILDKEGGLPKRERRSTQRLFEELRGRGYDGAHDSV
                     HRFVKAWRNERSRVPAQAYVPMSFAPGEAYQFDWSHETITLQGL"
BASE COUNT         1219 a         1747 c         1893 g         1170 t
ORIGIN      
        1 ggcgcgaaca ccagacggtt ggcggcgatg ccgtgcgctt ccgcctcgcg tttgagattg
       61 cgcattgcct ccgtattgtc ttcgagcaac cagagaacgc tcaccggaac tccatgcaaa
      121 agccgcatcc aaagcgcgaa gatctcgggg gtgattttgt aattgttgtt gaaacagcag
      181 aagacgaacc ccgaatccgg tagccccacc tcgcgccgcg tgaagcggtg gccggcgatg
      241 gcgcgccgcg aatcgttgca ttgatagctg tcgggaagat gaacgatctt ctcggtgtag
      301 taaggccgtt gatcatccgg aatgacgatc gcatcggcga tgatgtagtc gaaatagggc
      361 gcgcccatgg tgccgggata gcccagatag ctcgcttgaa tgggcgcggg ccgatgagcg
      421 aatatcccgg aacggtgatc cttggtataa cccttcagat caactgcgat atcgatctcc
      481 atctgccgca tcagaagggc ggtctccgca tcgctttttt cgcgcacgtc gatgaaacgg
      541 tcgaatgccg ccttgacgcg ggttcgcatc tcgcttttgt cgtcggggcc gaacgacaaa
      601 gcgatcagtt cgaagcggcc ccgatcgtgc tgttcgaaca caccggccat cagcgccggc
      661 gtggcatggg catgaaaatc ggccgatatg taggccacgc gaatcttttc gtggcgataa
      721 atctcgccgc gccagagtgg tgctggcgac agcggcgcct gatgcgcaac ccaaagccgc
      781 gcgcattgca attgatccgc cggcgaacgc gacagcgcaa cattgatgaa cggatagatg
      841 atgcgtttgc cggcggctag accgctggaa atcgcatttc tgtcggcttc gaaatggcgc
      901 caatcggagg cctgcatgcg gttgaggatg agattgccgc gcgcataagc gaattcggga
      961 tcgatgacga ggacacgctg aaaatcattc gcggcctcac catagcgctt cagcgcgcca
     1021 taagccgagc cgcgattgac aagcgcttca acggagcgtg gtacggatac aagcgccttg
     1081 ccgaaggcgg cgatggcgtc gtcgtaacgc cccattttgt gcagcacgca gccgcgattg
     1141 ttccaggtcc ccacattggc gggatcgagc gccagcgcga gatcgagact ttcgagcgcc
     1201 tgttcgtcgc ggttcagcgc catcaggcac atgccccgat tggtgtgtgc gtcggcgaga
     1261 ttgggcttca aagcgagcgc ttggtcgaag caggcaagcg cttcgggggc acgattcagc
     1321 cgttgaaaag cgcagccgcg ggtgaagaat gcttcgggca ctcgcggatt gatggcaatc
     1381 gcgtttgcga tcagacgctg cgcctcttcg aaccgccccg cctggtaata gacggcgccg
     1441 agcgcgaaca aagcctggaa atgccgcgga tcgccgcgta ggacctcgcc atagagacgc
     1501 gccgcttcgg aaagattgcc ggtctgatgg gcgcgcatcg cattttgaag aacggaatcg
     1561 ttcatgcaga agccgtctct gcgggacgtt cgccttcgtc agccgacgtc atttctaatc
     1621 gccgagcgcc acgccttcgc ggcgcggatc ggcgccgcct tcatatccgc cgctgacccg
     1681 gcgaatgccg ttcacgcccg aatcgaaggc aatcacgcgc acctgatggc ccatcgcggt
     1741 caaggcgggc gtcaactgat cgagcgcggt gccgcgctcg atcaaggtgg caccattcat
     1801 gttcacatgg cggggcgccg ccgcgacgtc cggcatcgag agcttggcat cgatcaggtt
     1861 gaccagagct tcggcgacat aggcgatgat ctgacggcca ccgggtgaac cgacggccgc
     1921 gaagaattgt ccgtcgggtg cgaagacgag tgtgggcgac atcgcgctca aggggcgttt
     1981 gccgggcgcc ggcgcatttg ccacgggctt gccgtcgatc accggctcga aggaaaaatc
     2041 cgtcagctgg ttgttcagca ggaatcctcc cgccgcgatc tgggctccaa agcccgcctc
     2101 caccgaagtg gtcatagaca gggcttcgcc gcgatcatcg accacggcca ggtgactcgt
     2161 gcccatttcg atctgcggcc gcatcggggc gtaatcgggc agttcggttt tttgcatcag
     2221 cggtctgccg gcgggcgcga cacccatgga tcgcatcgga tcgatcagcc gagcgcgcga
     2281 atcgagatag gcgggatcga gcagacctgc gagcgggacc ggaacgaagt cggtatcgcc
     2341 cagccattgg gcgcggtcgg cataggcaag gcggctggct tccgagacga gatgcacggc
     2401 cgataatgtg ctcggctgca attggctcga ggggaaatgc tgcaacagtc cgagaatctg
     2461 cagcacggtc acgccaccgg aggtggacgg cggtagcgag cagacgcgat aggcgcggta
     2521 ggtgccgcag agcggcgtgc gctccttggc gcgatatccg gcgagatcgg caagcgtcat
     2581 cgtggcggga ttgacgggcg cgttggccac ggcggcagtg atctcgctgg cgattgcgcc
     2641 cgtgtagagc gcgtccggcc cgttttgggc gatcttgcga aaggtctggg cgagcattgg
     2701 gttgcgccat gtttccccag ctttgatcgg cgtaccgtct gggtggagga actgcgcctt
     2761 tatggcgggc atggcggcga gccgcgggcc gccttgcgcg agttcgatcg ccagacgcgg
     2821 gggcaccttg aagccctcat ccgcaagctt gatggcaggc tcgaacagtt ttgcccaggc
     2881 aagccggcca tgggccgcat gcgccattgc cagcatccgc actgcgccgg gcacgccaac
     2941 ggaaaggccc cctggaacag catcattatg gcgccttggc ttgccgtcct tatccaggaa
     3001 catgtcgggc cgcgcggacg ccggtgcggt ttcgcgcccg tcataagcat gaattgccgt
     3061 cccatcggca acgatcagat aggcgccgcc gccgatgccg gaggattgag gctcctccaa
     3121 ggtcaagacc atctgcgccg cgatggcggc atcgaccgcc gacccgccat tgcgcagcat
     3181 cgcaaggccc gcctcagccg cgtccggttc ggccgccacg atcatatggt gctgggcggg
     3241 tgctgcggcc tgcgccggcg ggatctgaga cgcaaaaacc gccagaatcg gccagccggc
     3301 tatggtcccg gctatggtga aggtgcgcga aagccgtgcg aaagctgaag caacccgttt
     3361 catcatgaat ctcgtgcgga tctaaactta agctaacgca gtgtgccctg gtctgcccag
     3421 ccctttgcaa aggcggcgga tagtgagcgg gggacctcta tataatagga tgcgccctat
     3481 ggatgcctga ggcgggcgta atttcgggag tgggcgctga agataccggg cgcaccccag
     3541 ccggtgctcg gaggagagca cggaatatgt ccggcgcggc tttgggagtt gacgcttgcc
     3601 aattagatca gtgctgacta gtttcggcgc ctctcaacgc accggtagct cagctggata
     3661 gagcaccaga ctacgaatct gggggtcgtg ggttcaaatc ctgcccggtg cgccattcat
     3721 tccttcgcaa acttccggtt ggagaacact gctcggaaaa gcgcgaatgt gggacttttt
     3781 ctgcaagacc gcgccctgaa ctgcgcatag agactgagcg atcggtcaga tgtcggccat
     3841 tcgtccgtgt gtgtctccgt ttgccgagaa atgactccca gtttcgagct gaactggggg
     3901 ttacgcatgg ctgcgtggtc accaacccga accgaacttc gctcaaattc aaaaaatact
     3961 ggagaaattc gctctccccg accgcctcga tcggagtgtc agaaaagccc cgcatttccg
     4021 gattttctat tgcaatgaca agcttcgcag cgtgcaacat aactggagaa aatttgacga
     4081 taactggaaa atcgaaacaa tacataccgg aaaagcagca tcaaacctca gatgttgaca
     4141 cgtttcagag gaagggcgac cgatttaagg gcccaagaag ctctgctgcg cgcgccatga
     4201 aagcggaata acagcgtcga gcaaacgagc gaggcgaacg ttcggcgact gctcaccgct
     4261 gaggatggat ctggttagtg cggggctcag gaaagcgaga ttgagcgtac gtccgacatg
     4321 ccggcgctcc tgtccaagct tcaccgccaa ggcatccacc gagcccactt ccccctctat
     4381 cagcatccgt ttccagctat gggcccgtgc caacgctttg atcagcgagg catcgggcag
     4441 ggaggggcgc gaacccgcgc cggcggggtc taggatggat gcgcggccgc cgcggaaacg
     4501 tgcccggacc ggtaacgtga gaatgaggtg ctcaccatcg gtaagctctt caccgctggc
     4561 taggcgctca cgacaatcat cgacaaggac ttgtgcagtg gcgcgctgcg cttcggtgtt
     4621 cgtggctctc cacttttgaa ccgctgcctc ccgatcaaac tcgagggtca cggaattgga
     4681 tttgatagtt atcctgctca aaaccgaccg gtcgtcctga caagcgtctg ggctgcctga
     4741 tagctgcagc cgcgtaagcg cactttttat aaggtcctcc agcgcggcgg ctggaacgcg
     4801 ggaaatcgac acgttcgaat ggttaccctt cagcctcgat tggctcgtat agtagcggta
     4861 acgacggccg tcgggcttac gactgtaggt cggggtcatc aaagtgccct cggcgtcgaa
     4921 tagaattccg gtgagcatgg catcttggga agaccgagcc ttacctggct cgcgctttcg
     4981 gtaacctgta atgcgagctt gaacagcgtc gaacaggtct tgcgtgatta tcgcctgatg
     5041 ctctcccgga tacgaaacac ccttgtgcat ggttttgccg atatagaccc ggttcttgag
     5101 aaggcagtag agagcaccgc ggcccagcgg ttcgccgcta tgcgcgacac ccgtgctggc
     5161 ggtccatcgt ttcgatacga cgcccttggc tgctagatcg ccatgcaaag ccttcacgca
     5221 ccccagatcc agatatcgct tgaatatgag attgacggtc tcggcttcgg gggggtttac
     5281 aaccagcttg cgactatcga tgtcataacc cagtggagga tagcccccca tccacatgcc
     5341 cttcttcttg gaagcagcaa acttatcacg gatgcgctcg cccgtgacct ctcgctcgaa
     5401 ctgcgcaaaa gacaggagaa cattcagtgt gagccgtccc atggaggtcg tcgtattgaa
     5461 ggactgggtt gcaaaatcgc ttgcaatatt gaccccctta gggggctttt cgcgtccaat
     5521 tccgaccccc tttgatgtgt tgtgtcagcg tccttccttt tggccttgat cggcctggaa
     5581 gggctgacgg ggatgattgg cgtggacaag atcggcgaga tacggcgcgc ctactttgag
     5641 cagcaccgcc cgatcaagga gatcgtacga actctgtcgg tgtcgcgcgc gacggtgcgc
     5701 aaggtcatcc gaggtcatcg aaccgagttc aagtatgagc gcggtattca gcccacgccg
     5761 aagctcggcg attgggtcga ggttttgacg gagatcctgg ataaggaagg gggcttgccc
     5821 aagcgggagc gccgctcgac gcaacgcttg ttcgaggaac tgcgcggacg cggttacgac
     5881 ggcgctcacg acagcgtgca ccggttcgtc aaagcttggc gcaacgaacg ttcgcgggtt
     5941 ccggctcagg cctatgtgcc gatgagcttc gcgcctggag aagcctatca gttcgactgg
     6001 agccacgaga cgatcacgct tcagggtct
//
LOCUS       DASYEG010000214         6496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2677648, whole genome shotgun sequence.
ACCESSION   DASYEG010000214 DASYEG010000000
VERSION     DASYEG010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2677648"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VGM72_09575"
     CDS             complement(<1..287)
                     /locus_tag="VGM72_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY4265556.1"
                     /translation="MIAPVLPTYNRADIAFERGEGAYLFDERGERYLDFGSGIAVSAL
                     GHAHPHLVSVLREQAGKLWHTSNLYRVPGQERLARRLIDASFADTVFFANSG"
     gene            503..946
                     /locus_tag="VGM72_09580"
     CDS             503..946
                     /locus_tag="VGM72_09580"
                     /inference="COORDINATES: protein motif:HMM:NF019370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HEY4265557.1"
                     /translation="MGWTDERVEQLKTLWGEGLSASQIARVLGGVTRNAVIGKVHRLG
                     LAGRAGPPRAERPRNTVARKSVVHAPVPAPQIVEEDPITLEDGHFATVLTINDRMCRW
                     PIGDPSENEFHFCGHKPKSGSPYCEAHARKAYQPQPQRRDRRIAS"
     gene            complement(1029..1811)
                     /locus_tag="VGM72_09585"
     CDS             complement(1029..1811)
                     /locus_tag="VGM72_09585"
                     /inference="COORDINATES: protein motif:HMM:NF023204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HEY4265558.1"
                     /translation="MKRALAVLAALTISLPVLAHAEEQHDGREGRGGQHAQAPAPAQQ
                     NRAPAPQNRAPAPQQNQAGPRAGGFFGRVDPNAARQAAPNAAQERGRGAPNAAQVAPS
                     PSGTRPDVARERGRGAPNAAQVAPRQGRPDANVVENRDRDFRDGRRDDRGDFRNDRRD
                     DRRDFRGERDRGRFFNFRGRQYSAVRGSAFSYPRGWDYRHWDRGQFLPSVFLATPYYF
                     DYGLLGLPPPPPGARWVRNGPDALLVNVYNDRILDVIYDAFY"
     gene            complement(1894..2604)
                     /gene="phoB"
                     /locus_tag="VGM72_09590"
     CDS             complement(1894..2604)
                     /gene="phoB"
                     /locus_tag="VGM72_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEY4265559.1"
                     /translation="MAENGDPRVMVVEDESALSTLLRYNLEREGYRVFEAKDGEEALI
                     LADEVKPDLVVLDWMLPQLSGIEVCRRLRGRGHMRNVPIVMLTARGEETDRIRGLDTG
                     ADDYIVKPFSMTEFLARLRAVMRRVRPSLVENVVAVGEISIDRGSHRVRRASRDIHLG
                     PTEYRLLDQLMQHPGRVFSREQLLDSVWGSDVYVEARTVDVHVGRLRKALNDPGEKDP
                     IRTVRSAGYSFDETFAVQ"
     gene            complement(2611..3324)
                     /gene="phoU"
                     /locus_tag="VGM72_09595"
     CDS             complement(2611..3324)
                     /gene="phoU"
                     /locus_tag="VGM72_09595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY4265560.1"
                     /translation="MSEHIVKSFSEQLETLANAVAQMGGLAEAQFANAIEAVAKRDTK
                     LAEAAIGSDERVDRIQQTVEDQALRLLALRQPMAVDLRNTLAAIKIAYELERVGDLAK
                     NIGKRALVLNREPPIRLTQSLARMGRQALSQLKSVLDAYSDRDAEAAKSVWRHDEEID
                     ELYNSLFRELLTYMMEDPRTIGLCTHLLFIAKNIERAGDHATNIAETVHHMVTGAYLR
                     IDRPKADLTSITPIPHEPN"
     gene            complement(3427..4704)
                     /locus_tag="VGM72_09600"
     CDS             complement(3427..4704)
                     /locus_tag="VGM72_09600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4265561.1"
                     /translation="MSEAAQAPSSGWRKSYLLLFAVAAVALLAVMVCGNAAVPFSILT
                     LAAIAGLTFFLAVPRRTGPGGIAHLQPVQDAAMLTDSAKDILECVDEPVLILDPQGRV
                     VFANKASQPLVGADAEKKHISAVLRTPEVLEAVERVLGGASSETIAFSVLVPVQHHYE
                     AHITRTGGFLVLVRVRDLTEIRRAEELRADFVANASHELRTPLAAVTGFIDTLRGHAR
                     DDTPARERFLEIMSVEAARMRRLIEDLLSLTRIELNEHNPPAGEVDVAAVIHDVVAVL
                     SPLTAEGRIVIQDAKEPLMVVGDRDELTQVFQNLIHNAIKYGRAGGEVRVRFARAEAP
                     SKREGAGQVVVAIQDEGEGISREAIPRLTERFYRVDVKRSRERGGTGLGLAIVKHILN
                     RHHGRLQIESVPGDGSTFTVFLPGVSLPAAGHN"
     gene            4818..5483
                     /locus_tag="VGM72_09605"
     CDS             4818..5483
                     /locus_tag="VGM72_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265562.1"
                     /translation="MPISSDLLNDFIEEKLSPEETDAIRATIAGDPELAAYVQDRKAL
                     KAALASRPAAWLGRIHGRVAGQSASWIPACAMAAGIGLGILLAGTFGIGTDVRGQDGT
                     LIAQGELGRVLSTALTGEQSAEPAARIAASFWSKNGSFCRSFVTRGTSMSALAGIACR
                     EQGAWRIAATAAVPPIDPRQSRISPEELPPSVRGVMDNLIVGRPLDADAERQLRNQAW
                     RAR"
     gene            5777..>6496
                     /locus_tag="VGM72_09610"
     CDS             5777..>6496
                     /locus_tag="VGM72_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265563.1"
                     /translation="MRTFKNSLATALLCGAVGLAYPAVAQVGGAGGGTGAGGVAAGAG
                     TSVGTTGGGNNSVGSATSGTLNNTATGALGVPGSGAVGGAATNRGAGTAINSASTNTL
                     NNTATGALGSTGSPTGSSLGSTGSNAAANAGVTAGGSSTPAGTTNGVSNSSLPGTTGA
                     ATATGDGAANNYGADNNAAAAGAIGSSPSGNTPNFLANVQSTPTPAKGLGKAAMARVD
                     QNERRTTSELNRASANSTGNAS"
BASE COUNT         1259 a         2030 c         2048 g         1159 t
ORIGIN      
        1 ccggaattgg caaaaaagac cgtgtcggcg aaactcgcgt cgatcaggcg gcgcgccagg
       61 cgctcctgac cgggcacccg gtagagattg gaggtatgcc agagtttgcc cgcctgttcg
      121 cgcaacaccg aaacgagatg cggatgcgcg tgaccgaggg ccgagaccgc gatgcccgag
      181 ccgaaatcga ggtagcgttc gcctctttca tcgaagagat aggctccctc gcctctctcg
      241 aaggcgatgt ccgccctgtt gtaggtcgga agaaccggcg caatcacgaa agccctcctt
      301 ggcgggggta aaaacgaaaa cgcgacttat aatgtccgac gctgcaaagc agaagccgca
      361 aagtctcagg ttttgacaaa ttgcgcgacg atgctttggc ccctagcgac ctgtggacaa
      421 ggtttggtgg ccccaccgca taacttgtgt tccgaacggt gtctgctacg atattttgta
      481 ggtttaagcc aggagaagcg gcatgggctg gacggacgaa cgggtcgagc aattgaagac
      541 gctttggggt gaaggacttt ccgcgagtca gattgcgcgc gtgctcggcg gcgtcacgcg
      601 caacgcggtc atcggcaagg tgcatcgcct gggcctcgcc ggccgcgccg gtcccccgcg
      661 cgccgaacgg ccaaggaaca ccgttgcgcg caaatcggtc gtgcacgcgc cggttcccgc
      721 gccccagatt gtcgaggagg atccgatcac attggaagac ggccatttcg ccaccgtact
      781 gaccataaac gatcgcatgt gccgctggcc cattggggac ccatcggaaa atgaatttca
      841 tttctgtggg cacaagccaa aatccggatc gccttattgc gaggcccatg cccgcaaagc
      901 ctatcagccg caaccccaac gccgggaccg gcgcatcgcg agttagacca gatcacaggc
      961 gagttaacca gatcacagga aaaagcccgg gcgcgaaagc atccgggctt ttttcatgcg
     1021 atgctcaatc agtaaaacgc gtcgtagatc acatccagga tacggtcgtt gtaaacattc
     1081 acgagcagcg catcgggacc attgcggacc cagcgcgcac cgggcggcgg cggcggcaaa
     1141 ccgagcaagc cgtaatcgaa ataatagggc gtcgccaaaa agacgctggg cagaaattgg
     1201 ccgcgatccc aatggcgata gtcccagcca cgcggatagg agaacgcaga tccccgcacg
     1261 gccgaatatt gccgcccgcg gaaattgaag aaccggccgc gatcccgctc gccgcgaaaa
     1321 tcccgtctgt catcgcgccg gtcgttgcgg aaatcgcctc tgtcgtcacg tctgccgtcg
     1381 cggaaatcgc gatcgcggtt ctccacgaca ttcgcgtcag gccgtccttg ccggggcgcg
     1441 acctgtgccg cgtttggagc gccgcgtccg cgttcgcgcg caacatcggg ccgcgtgcct
     1501 gacggcgaag gcgcgacttg cgccgcgttc ggagcgcctc ggccccgttc ctgcgccgcg
     1561 ttgggcgcgg cctgccgcgc cgcgttcgga tccacccgtc caaaaaatcc cccggcgcgc
     1621 ggacccgctt gattctgttg cggcgcgggc gcgcggtttt gcggcgcagg tgcgcgattt
     1681 tgctgcgcgg gcgcaggcgc ttgcgcgtgc tgtccaccgc gtccttcccg tccgtcgtgc
     1741 tgctcctcgg catgcgcaag aacgggaagc gaaattgtca gtgccgcaag aacggccaga
     1801 gcgcgtttca tgaaatcctc catctattac cggttgaaac gcccgaatag atagggagtt
     1861 caaagaaaag cggtcaagcc cacgctggtc cggctattga acggcgaatg tttcgtcgaa
     1921 cgaatagccg gccgaccgca cggtgcggat gggatccttc tcacccggat cgttgagcgc
     1981 cttgcgcaga cgtccgacat gcacatcgac ggtgcgcgct tcgacataga cgtccgagcc
     2041 ccacacactg tccagcaatt gctcgcgtga gaagacgcgc ccgggatgct gcatcaattg
     2101 atcgagaagc cgatattccg tcgggcccag atggatgtcg cggctcgcgc gccgcacgcg
     2161 atgactgccg cggtcgatgc tgatctcacc caccgccacc acattctcga ccaggctcgg
     2221 gcgcacgcgg cgcatcacgg cgcgcaggcg cgccagaaat tcggtcatcg aaaacggctt
     2281 gacgatgtaa tcgtccgcgc cggtatcgag cccgcggatg cgatcggtct cttcgccgcg
     2341 tgcggtcagc atcacgatcg gaacgtttct catatggccg cgcccgcgca agcggcggca
     2401 gacctcaatc ccggagagct gcggcaacat ccaatcgagc accaccagat cgggcttcac
     2461 ctcatcggca aggatcagcg cttcctcgcc gtcctttgcc tcgaagacgc ggtaaccttc
     2521 gcgttcgaga ttgtatcgca gcaaggtcga aagagccgat tcgtcttcca cgaccataac
     2581 gcgaggatcg ccgttctccg ccatgttggt ttagttcggc tcgtgaggga tgggcgtgat
     2641 cgaggtcaga tcggctttgg gccgatcgat cctgagatag gctccggtga ccatgtgatg
     2701 cacggtctcc gcgatattgg tcgcatgatc gccggcccgc tcgatattct tggcgatgaa
     2761 aagcaggtgc gtgcacaacc cgatggtgcg cgggtcttcc atcatgtagg tgagaagctc
     2821 gcggaacagg ctgttataca attcgtcgat ctcctcatcg tgacgccaca ccgatttggc
     2881 ggcttcggca tcgcgatcgg aataggcatc aagcacggac ttcaattgac tcagcgcctg
     2941 acggcccatg cgcgcgaggc tctgggtcag ccggatcggc ggttcgcgat ttaagaccaa
     3001 cgcgcgcttg ccgatattct tggccagatc gccgacgcgc tccagctcat aggcgatctt
     3061 gatggcggcg agcgtgttgc gaaggtccac cgccatgggt tggcgcaagg ccagaagccg
     3121 caatgcctgg tcctcgaccg tctgctggat gcggtccacg cgctcgtccg agccgatcgc
     3181 tgcctcggca agcttggtat cgcgcttggc caccgcttcg atggcgttgg cgaattgcgc
     3241 ttccgcgagg ccgcccatct gagcgaccgc gtttgcaagc gtctccaatt gttcactgaa
     3301 agatttcacg atgtgctcgc tcatcggccc ctcctggcgc aatgccagtc aattgcgctg
     3361 taggacaatt ccgtcacgcc tttgcaacaa atatgtaaca gtttgatgac gcgcttgtcc
     3421 gcgcgttcag ttatggcccg cggccggcaa cgagacaccc ggcagaaata ccgtaaatgt
     3481 actcccatct cccggcacgc tctcgatctg cagccgtccg tgatggcggt tcaatatgtg
     3541 cttcacgatg gcgagcccca aacccgtgcc gccgcgctcg cggctgcgct tcacatcgac
     3601 gcgatagaat cgctccgtca aacgcgggat ggcttcgcgt gaaatgccct cgccttcgtc
     3661 ctgaatcgca acgacgacct gccccgcgcc ttcgcgtttg ctcggcgcct cggcgcgcgc
     3721 gaatcgcaca cgcacctcgc ctccggcacg gccgtatttg atggcgttat ggatcaggtt
     3781 ctggaacact tgagtcagct cgtcccggtc gcccaccacc ataagcggtt cctttgcgtc
     3841 ctggatgacg atgcggcctt ccgccgtcag gggtgacagc accgccacca cgtcgtggat
     3901 caccgccgcc acatccactt cgcccgccgg gggattgtgc tcgttgagct cgatccgcgt
     3961 gagggacagc agatcttcga tcagccgccg catgcgcgcc gcttccacgc tcatgatttc
     4021 gaggaatcgt tcgcgcgccg gcgtgtcatc cctggcatgg ccgcgcaggg tgtcgatgaa
     4081 gccggtgacc gcggccaagg gcgtgcgcag ctcgtggctg gcattggcga cgaagtccgc
     4141 ccgcagttct tcggcgcgac ggatttccgt aagatcgcgg acacgaacca ggaccagaaa
     4201 gccgcctgtg cgcgtgatat gcgcttcgta atgatgctgc accggcacga gcacgctgaa
     4261 ggcgatggtt tcggaactgg ccccgccaag gacgcgttcg accgcttcca ggacctcggg
     4321 cgtgcgcagc accgcggaga tgtgcttctt ctccgcgtcc gcgccgacca atggctggga
     4381 ggctttgttg gcgaaaacga cccggccctg agggtcgagg atgagcaccg gttcgtcgac
     4441 gcattccagg atatctttgg ccgagtcggt cagcatcgcg gcgtcttgga ccggctgcag
     4501 atgagcgatg ccacctgggc ccgtgcgtct cggcacggca aggaaaaagg tcaggccggc
     4561 gatggccgca agcgtcagga tggagaacgg gacggccgcg ttgccgcaaa ccattacggc
     4621 gagcagagcg acggcagcca ccgcaaagag caggagatag gatttccgcc aaccgctcga
     4681 aggcgcctgg gcggcttcgc tcatgatcat cgatccttcg tccaaaatgg aggtcgcgcg
     4741 cttatagcac gcgtctgtgc catttccgtg agacctcgca tcgcttcatc ttctttggct
     4801 ttctcttata atgggccatg ccgatttcat ccgaccttct gaacgatttc atcgaagaaa
     4861 aattaagtcc ggaggagact gacgccatta gggcgacgat cgccggcgat ccggaacttg
     4921 ccgcttatgt gcaggaccgc aaggcgttga aagcggcgct cgcttcgcgg ccagcggcct
     4981 ggctcggccg gatccatggg cgtgtcgcgg gacagagcgc gagctggatt ccggcatgcg
     5041 cgatggctgc cgggatcggc ctgggcattc tgctcgcagg cacattcggc atcggcaccg
     5101 atgtgcgcgg tcaggatggt acgctgatcg ctcaaggaga gcttggccgt gtcctctcaa
     5161 ccgcattgac cggggagcaa agcgcggaac cggcggcgcg catagcggcc agcttctgga
     5221 gcaagaatgg ttccttctgc cgcagcttcg tgacgcgcgg aacctcgatg agcgcgctcg
     5281 cggggattgc ctgccgcgag cagggtgcat ggcgcatcgc cgcgacggcc gcggtgccgc
     5341 cgatcgatcc gcgccaatcc cgaataagcc cggaggagct tcccccttcg gtgcgcggcg
     5401 tgatggacaa tctgattgtc ggccggccgc tggatgccga tgcggaacgt cagttgcgca
     5461 atcaagcctg gcgcgcgcgc tgattgcggg ttcctggatc gattaatcct tgtactgatg
     5521 agaacggcag cgcggcagcg cgaagcggca gcagggccgg atactcacgc tggttcttaa
     5581 ggttcctccc gcagcgggga tttttttcac gcgccgcggg agagaaatcc tcggcacagc
     5641 cgttgtcaga ggcagagaaa gcttccgagc tgcactacgg cccgtgaaga tattcggcat
     5701 cggccggaaa aagcccaaat cattgggaaa accccacatc gttgggaacc caacgtcggg
     5761 aaaaaaggag cacaacatgc gtacattcaa gaattcactc gccacagcgc tgttatgcgg
     5821 cgcggtgggc ttggcctatc cggccgtcgc gcaagtgggc ggagccgggg gaggaaccgg
     5881 ggcgggtggt gtcgcggcgg gagcgggtac cagcgtgggc acgaccgggg gcggaaacaa
     5941 ttccgtgggg tcggcaacgt ccggtacgct gaacaacacc gccaccggag ccttgggcgt
     6001 tccgggttcc ggggccgtgg gcggagcggc aaccaatcgg ggcgcgggca cggccatcaa
     6061 ttcggccagc accaacaccc tgaacaatac cgccacgggc gcgctcggga gcacgggctc
     6121 gccaaccggc agcagtctcg ggagcaccgg cagcaatgcc gccgcaaacg ccggcgtgac
     6181 cgcgggcggc agcagcactc cggcgggtac gacgaacggc gtgagcaata gctcattacc
     6241 ggggactacg ggcgccgcga ccgcgacggg agacggtgcg gccaacaatt atggggctga
     6301 taataatgct gcggcggctg gagccattgg cagcagcccg tcgggcaaca ccccgaactt
     6361 ccttgcaaac gtgcaaagca ccccaacgcc ggcgaagggg ttgggcaagg cggcaatggc
     6421 acgcgtcgat cagaacgagc gccgcaccac gtcagagttg aatcgcgcct ccgccaacag
     6481 caccggcaat gcgagc
//
LOCUS       DASYEG010000215         1678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3838464, whole genome shotgun sequence.
ACCESSION   DASYEG010000215 DASYEG010000000
VERSION     DASYEG010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3838464"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VGM72_09615"
     CDS             complement(<1..833)
                     /locus_tag="VGM72_09615"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY4265564.1"
                     /translation="MSELGIAHPIVLAPMAGAGGTPELVAAVSNAGGLGSWGAAYATP
                     QQILDTAKQIRGLTSKPFALNLFAGGYEPQRHVDPAPMLALVTRVHEKLGLAPPVVPP
                     NPASPFEDQVAAVIEARPAVFSFTFGIPSADALARLRKAGIQIAGTATTVAEGKQLAS
                     AGVDWIVAQGEEAGAHRGSFLDAFEQSMVPMRELTRELLREVSVPVIASGGIMDGRDI
                     VEMLDAGAVAAQLGTAFLVCDESGAPDAYKKALLAAKDDTTVITRVYSGRPARGLRNT
                     F"
     gene            complement(943..>1678)
                     /locus_tag="VGM72_09620"
     CDS             complement(943..>1678)
                     /locus_tag="VGM72_09620"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265565.1"
                     /translation="VALSFASAILQAFTAIAIVGIAAVLLGATAKVLGDTVRVIEIVS
                     YTLIVLIGLRLLWVKGRAFLKLLVPAKPEHAHAHHDQGHDHHHGHGHRDHGHAHSHSH
                     SHAHHDHDHGDEAHAWGHAHAPEPNELKGRDWWKRGLAAIVAVGLRPCSGAIIVLVFA
                     LAQGLFWIGVASTFAMGLGTFITVALIATLAVGARGLAGRWAKAKPGAGMLVLRGLET
                     GAAIVIIAFGGLLLTGYIVSERLMAG"
BASE COUNT          320 a          566 c          528 g          264 t
ORIGIN      
        1 atgaacgtat tgcgcaagcc ccgcgcgggc cggcccgaat agacgcgcgt aataaccgtg
       61 gtgtcgtctt tcgccgccag caaagctttc ttataagcat ccggagcgcc gctttcgtcg
      121 cacaccagaa acgccgtacc gagttgcgcc gcgaccgcac ctgcatccag catttcgacg
      181 atgtcgcggc cgtccatgat gccgccggag gcgatcaccg gaactgaaac ttcacgcaga
      241 agctcgcggg tcagttcgcg catcggcacc atcgattgct cgaaggcatc aagaaagctg
      301 ccgcgatgcg cgcccgcctc ctcgccttgt gcgacaatcc agtcgacgcc ggcggacgcg
      361 agttgcttac cttccgcgac ggtcgtcgcg gtgcccgcga tctgaatgcc ggccttgcgc
      421 agccgcgcca gtgcatcggc gctggggatg ccgaaggtga aactgaacac ggcaggccgc
      481 gcttcgatca ccgcagcaac ctgatcctca aacgggctcg ccgggttggg cgggacgacc
      541 ggcggcgcga gacccaattt ttcatggacc cgcgtgacca gcgccagcat cggcgctgga
      601 tcgacgtggc gttgcggctc gtagccgccg gcgaacagat tgagcgcgaa aggcttgctc
      661 gtcagcccgc ggatctgttt ggcggtgtcg agaatttgct gcggcgtcgc gtaagccgcg
      721 ccccacgaac caaggccgcc tgcgttcgac accgcggcaa cgagttccgg cgtgccgccc
      781 gcgcccgcca tcggcgcgag gacgatcggg tgcgcgatac ccagttcgct caagaagttt
      841 cccgccatac gatgcctccc ttgggcagag gtacgctagc gctactcgca cggcgtacaa
      901 tacgtatgtc gcgatagcag ccttaaaacg cggcgatggg cctcagccgg ccatcagccg
      961 ttcgctgacg atatagcccg tcagcagcag cccgccgaaa gcgatgatga caatcgccgc
     1021 gccggtttcc agacctcgca gcaccagcat ccccgcaccc ggcttggcct tggcccagcg
     1081 gcccgcgagg ccgcgtgcgc ccactgccaa tgtggcgatc agggcgacgg tgatgaaggt
     1141 gccgagcccc atcgcgaagg tcgacgcgac accgatccag aacagccctt gcgccagcgc
     1201 gaagacgagc acgatgatcg cgcccgagca cggccgcaag ccgacagcga cgatggccgc
     1261 cagcccgcgc ttccaccaat cacgcccctt gagttcgttc ggttccggcg cgtgggcgtg
     1321 gccccaggca tgcgcctcat cgccatgatc gtggtcatga tgcgcatgag aatgagaatg
     1381 gctgtgcgca tgaccatgat cgcgatggcc gtggccatgg tggtgatcgt gcccctggtc
     1441 gtgatgcgca tgcgcgtgct caggcttcgc cggcacaagc agcttcagga aggcgcggcc
     1501 cttcacccac aataaccgca agccaatgag aacgatcagc gtatagctga cgatctcgat
     1561 gacacgcacc gtgtcgccca ggaccttcgc ggtggcgcca agcagcaccg cggcgatgcc
     1621 gacgatggca atggcggtga aggcctgcag gatggcagac gcaaacgaca gcgcgacg
//
LOCUS       DASYEG010000216         7745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2969350, whole genome shotgun sequence.
ACCESSION   DASYEG010000216 DASYEG010000000
VERSION     DASYEG010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7745
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2969350"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..1371
                     /locus_tag="VGM72_09625"
     CDS             22..1371
                     /locus_tag="VGM72_09625"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HEY4265566.1"
                     /translation="MSSPLAIGAVSAVLRNLLDNGLIEAGAAMGTTVNISAVAPDTID
                     LDNAEEPPRLNLFLHQVTSNSGWRNAALPSRDSITGERLTNAPLALDLHYLLTAYGRA
                     DFHAEILLGYAMHLLHERPVLDRAAIRRALDPSPLDVSMLPPAYQALAASDLADQVEQ
                     IKITPAAMGHDEMSKLWSAIQTHYRPTSAYQVSVVLIQGTKPGRSPLPVLSRGLVDPV
                     TKRDRGVVVNPDLLPPLPTLFTLTPPASKSGATLGDTVTITGVRLIGAGHRVHLTHRL
                     VTTPVELTPAAPNAAGTEIKVTLPNDIAAQSTFVPGQWSLSIVFTPAGELNPRETNAI
                     PLVLAPVPVIVADVPLGLPAANVTRGGVPPRVTVTLTSRPQTRPQQAASLVLDTTEAK
                     ALPRVNAADPLVFEFANTLVSGNHWLRLRVDGTDSVLLDKSGPAPIFDPTQQIVVPP"
     gene            1368..3347
                     /locus_tag="VGM72_09630"
     CDS             1368..3347
                     /locus_tag="VGM72_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4265567.1"
                     /translation="MNALGGTSAGLAGQFASWSEANQHWLIAAIGRLRARLEAPSQTA
                     SETSDSVAAGSDCGFTPALLHCARAFNLSTFERELLLLAAGLELDAGLRAAVAALNGN
                     APARASYGLALGLLTQPHWDALSPDAPLRHWHMIELEAGVPLTQASLRIDERILHFLA
                     GVAASDARLSGIARYVPVPALDDGVDMALAQQVSQNLGADERGRIVAVYSEIHDICAR
                     RDLALAAVARLAQPALWIASRDLPTEPGELAAIARRVDREAALTGAVPILATEESGSE
                     PVALSFATRLQAALLWLGAPPAGLKALPEQHRVSIIALPMADAERTHTSLMSKWRRIA
                     GSTEGEDAGIATEFNRAATQFRITSAAMDDVMDSLAAIAPAERSAAVWPAVREGARGG
                     LDSLAQRIVTRVAFGDLVLPNAQIAILRDIARHLRNRERVYRDWGFGDKHARGKGLTA
                     LFCGESGTGKTLAGEAIANDAGLDLYRVDLAMLVSKYIGETEKNLKRLFDAAESSGAT
                     LLFDEADALFGKRSEVKDSHDRYANIEVSYLLQRIEAYRGLAILTTNMKSALDRAFLR
                     RIRFVINFPFPDAPARERIWRQQFPAGAPLKNVDFAALARLNLAGGHIHSIALNAAFK
                     AADREGPIDQGVLMEAARAEYAKLERSFTDGGGGS"
     gene            3344..3586
                     /locus_tag="VGM72_09635"
     CDS             3344..3586
                     /locus_tag="VGM72_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265568.1"
                     /translation="MNRKREASSPLHLNIGRIVVDADALGDVSRSRFRAEIGTALSQH
                     LSGQTIDGPPTLAQQIARSVAPQVRAQLPKGGRNGA"
     gene            3576..5780
                     /locus_tag="VGM72_09640"
     CDS             3576..5780
                     /locus_tag="VGM72_09640"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEY4265569.1"
                     /translation="MALSPAVQAIAKTGARPMRVLQRACSGKSCEECESCRKKKPLQR
                     EAAGGRGGNVPASYNAPSALTGGGVHLPVQLRQALGPLYGSDFSGVRIHHDAASHAAA
                     REVNARAFTLGQHVHFAAGEYRPGHQDGLRLLAHELGHTVQQRHAGDVDSGTEVEIGR
                     ADSALEREADAAADAVLDGRKARLSSIGAGGVQAKLLQRQLGTAAGGSESASVDRKVD
                     ENTIVHITRTVTEKKCTLDTDPDSTPTDKIFYWDKDANAIGLNYKICNGRVQLSTKGE
                     ISYDKVIDSAKNLLTTLQKNPALGQDLGNLLNDRLSAATISTSGDVTLTVDGILSASV
                     QTGSTTGTQNQQYNVRGVLKITPKGMSFTVTGGVDFSKTPLQSTTTYTLEGKVATEHF
                     AVDLKYEQIDTSKAGGAATSKGQTTLGVDVPLPDVGPLSGVTLGPTVTFDEHGNPTIG
                     GGLKGHFGKPETTPRVRCFKCNCPPPLPEYSCTKEVTAHREPVTKEAAKDSTVKLLYD
                     YNSAVPANAADFKSHVSSIAALVGQGYKVEHIWGYASPEGSLDTPKPPVPGFKGNIAL
                     SGRRASQARGEISTAAPGAALPEAEGKGEQLGDLDGTGDTADKDITPDLVKLLQSKTS
                     DDERLDVLGVEESVRNDAAKKAQAIADINAFIEGRDAKGLKLAQRPRWGKVFPFLRRV
                     EVALHHDPVIEMNPVAASNKKQNCEDADIAYARANMPALPPERRLPKEECGT"
     gene            5777..6436
                     /locus_tag="VGM72_09645"
     CDS             5777..6436
                     /locus_tag="VGM72_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265570.1"
                     /translation="MSFDISPKIIKGGLVVLAPGGGAVQRTIALQYNPDTLTRSYQVQ
                     GVGGDGGGERAQPFRLKGPAIESIKLEADIDATEQLELPDQNPNAVAFGIAPQLAILE
                     ALVNPSVDELLSNAAQSANGTLEIIPPEAPLVIFVFSKNRVVPVRVTDFSITEEAFDV
                     QLNPIRAKVNLGLRVMSTDDLGFQHKGGTIFLGYLRAREALAAKSGTANLSTLGITNL
                     A"
     gene            6450..6800
                     /locus_tag="VGM72_09650"
     CDS             6450..6800
                     /locus_tag="VGM72_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265571.1"
                     /translation="MTTDPVQLLIEAGAIPSSPFAANSRYYSVVLGRYQRDANDAGIT
                     YVLRRFIPQQRDIPIAGAHIVHGGERPDLLAAQALGDAGLYWRVADANAVTDPFELTD
                     TLGARIAIPTPPGT"
     gene            6815..>7745
                     /locus_tag="VGM72_09655"
     CDS             6815..>7745
                     /locus_tag="VGM72_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265572.1"
                     /translation="MNGGVQLSLQIGPVPIPAPREVVDALVHVKVEDGSGGTQSGFEL
                     GFELPIRSPLRTLFLLTGGGSLPLMRVVLIVTINGAARSIIDGVTTHVETQPGEGGVA
                     KLIVKGKDLSALMDLIEIPGLPFPAMPPSARVLLILAKYAAFGVIPMVIPSILDIPPI
                     PVQQIPQQRGSDYAYVTKLAKDAGYVFYLEPGPAPGTSKAYWGPEIRVGEPQPALTTN
                     MDALSNVDQISFNFDKEKKTIPIVFFQEPVSKIPIGIPIPDVTPLNPPLGLVPPLPPK
                     IVKLDSTAHLSAPDALMAGLAYAGQHSDSVFGKG"
BASE COUNT         1470 a         2531 c         2409 g         1335 t
ORIGIN      
        1 ctcccagcgc ggaggcaact catgagttcg ccgctcgcga tcggagctgt cagcgccgta
       61 ttgcgcaacc tcctcgataa cggtctgatc gaggcgggtg ccgcgatggg tacgacggtc
      121 aacatcagcg cggtcgcgcc cgacacgatc gatctcgaca atgccgagga gccgccgcgg
      181 ctcaatcttt ttctgcatca ggtgacgtcg aattccggct ggcgcaacgc cgcccttccg
      241 tcgcgcgact cgatcaccgg cgaaaggctg accaacgcgc ctttggcgct cgatctgcac
      301 tatctgctca cggcctatgg ccgcgcggat ttccacgccg agatcctact cggttatgcg
      361 atgcatcttc tgcacgagcg gcccgtcttg gatcgcgccg ccatccggcg cgcgctcgat
      421 cccagtccgc tggatgtcag catgctgccg cccgcctatc aggcgctcgc ggcgtccgac
      481 ctcgccgatc aggtggagca gatcaaaatc acgcccgccg ccatgggcca tgacgaaatg
      541 tcgaagctct ggtcggcgat ccagacgcat tatcgtccga cctccgcgta tcaagtctcg
      601 gtggttctga tccaaggcac caagcccgga cgctcgcctt tgccggtgtt gtcgcgcggc
      661 ctcgtcgatc ccgtgaccaa gcgcgatcgc ggggtggtgg tgaaccccga tttgctgccg
      721 cctctgccca cgctgttcac gctcaccccg cccgcgagca aaagcggcgc gacgctgggc
      781 gataccgtaa ccattacggg cgtgcgcctc atcggtgcgg gtcatcgcgt gcacctgaca
      841 catcggctgg tgacaacgcc ggtggagttg actccggcag cgcctaacgc cgccggcacg
      901 gagataaaag tcaccctgcc caacgacatc gcggcgcaga gcacgttcgt tcccggacaa
      961 tggtcgctga gcatcgtctt tacgccggcg ggcgagctta atccgcgtga gaccaatgcg
     1021 atcccgctgg tcctcgcgcc tgtccccgtg atcgtggccg atgtgccgct gggccttcct
     1081 gccgccaatg tgacccgcgg cggtgtgcct cctcgtgtca ccgtcacgct gacatcgcgc
     1141 ccgcagacgc gtccgcaaca ggccgcgtcg cttgtcctcg acacgacgga ggccaaagcg
     1201 ctgccgcgcg tcaatgcggc cgacccgctg gtgttcgaat tcgccaacac gctggtttcc
     1261 ggcaatcatt ggctgcgcct gagagtcgat ggcaccgaca gcgtgctgct cgacaaaagc
     1321 ggcccggcgc cgatattcga cccgacccag cagatcgtgg tgccgccatg aatgcgctcg
     1381 gcggtacatc ggcgggtctg gccggacaat tcgcgagctg gtccgaggcc aatcagcatt
     1441 ggctgatcgc agcgatcggg cggttgcggg cgcggcttga ggcgccgtca caaacggcat
     1501 ccgaaacctc agacagcgtc gcggccgggt ccgattgtgg tttcacaccg gcgctcctgc
     1561 attgcgcgcg cgcgtttaac ctgtcgactt tcgagcgcga actgctgctg ctggcggcag
     1621 gcctcgaact cgatgccggc ctgcgtgccg ctgttgccgc gctgaacggc aacgcgccgg
     1681 cgcgcgccag ctatgggctc gcgctcggtc tgctcacgca gccgcattgg gatgcgctat
     1741 cgcccgacgc gcccttgcgc cattggcaca tgatcgaact ggaagccggt gtacctctca
     1801 cgcaagcatc tttgcgaatc gatgaacgca tccttcactt tcttgccggt gttgcggcaa
     1861 gcgacgcacg gctttcgggc atcgctcgct atgtgccggt tccggcgctc gatgatggcg
     1921 tcgatatggc gctcgcccaa caggtctcgc aaaacctggg cgccgacgag cgcggacgca
     1981 tcgtggcggt ttatagcgaa atccatgata tctgcgcccg gcgggacctc gcgctcgcgg
     2041 cggtcgcgcg actggcgcag cccgcgctct ggatcgcaag ccgtgatctt cccaccgagc
     2101 ccggcgaact tgccgcaatc gcacgccgcg tggatcggga agccgcgctg acgggcgccg
     2161 tcccgattct cgcaacggaa gaatcgggtt cggagccggt ggcactttct ttcgccactc
     2221 ggttgcaggc cgcgctgctg tggcttgggg ccccgcccgc gggtttgaaa gcgctaccgg
     2281 aacagcaccg cgtttcgatc atcgcgctgc cgatggccga tgccgagcga acccatacga
     2341 gcctgatgtc gaaatggcgc cgcattgcgg gttccaccga aggcgaggat gcagggatcg
     2401 cgacagagtt caatcgcgcc gccacgcaat tccggatcac ttcggcagcc atggacgatg
     2461 tcatggacag tctcgcggca atcgctccgg cggaacggag tgcggcggtc tggcccgccg
     2521 tccgggaagg cgcacgcggc gggctcgatt cgctcgctca gcgcatcgta acgcgcgtgg
     2581 cgttcggcga tctggtgttg cccaatgccc agattgcgat cctgcgcgat atcgcgcggc
     2641 atctgcgcaa tcgcgaacgc gtctatcgcg attggggatt cggcgacaag cacgcccgcg
     2701 gcaagggcct gaccgcgctc ttctgcggcg aatccggcac cggcaagacg ctggccggcg
     2761 aagcgatcgc caatgatgcg gggctcgatc tctatcgtgt cgatctcgcg atgctggtca
     2821 gcaaatatat cggcgagacc gaaaaaaatc tgaagcgtct gttcgatgcc gctgaatcca
     2881 gtggtgcgac acttctgttc gatgaggccg acgcgctgtt cggcaagcgc agcgaagtca
     2941 aggacagcca cgaccgctac gccaatatcg aggtctctta tctcctgcag cggatcgagg
     3001 cctatcgcgg tcttgccatc ctgacgacaa acatgaaaag cgcgctcgat cgcgcgttcc
     3061 tgcgccggat tcgtttcgtc atcaatttcc cctttcccga cgcacccgcg cgcgagcgaa
     3121 tctggcgcca gcaattcccc gccggtgcgc cgctcaagaa tgtcgatttc gccgcgttgg
     3181 cgcggctcaa tctcgcgggc ggccatatcc acagcatcgc gctcaatgcg gcgttcaagg
     3241 ccgcggaccg ggagggccca atagaccagg gggtgttgat ggaagccgcg cgcgccgaat
     3301 acgcaaagct cgagcgcagt ttcaccgacg gcggaggcgg gtcatgaatc gcaagcgcga
     3361 ggcatcttcg cccctccatc tgaacatcgg acggatcgtc gtggacgccg acgcgctcgg
     3421 cgatgtttcc cgctcccggt ttcgcgcgga gatcggcacc gccctctcgc aacatttgtc
     3481 gggccagacg atcgacggtc ccccgacact cgcacagcag attgcaaggt ctgtcgcacc
     3541 gcaggtgcgc gcgcagcttc ccaagggagg ccgcaatggc gcttagcccg gcggtccagg
     3601 ccatcgccaa aaccggcgcg cgtccgatgc gtgtcctgca acgcgcctgc agcggcaaga
     3661 gttgcgagga gtgcgaaagc tgccgcaaga aaaaaccgct gcagcgcgaa gccgcgggcg
     3721 gccgcggcgg gaacgtgccg gcctcctaca acgcgccgtc cgcgctcacc gggggcggcg
     3781 tccacctgcc ggtacaactc cgccaggcat tgggaccttt gtacggcagc gatttctcgg
     3841 gcgtgcgcat ccatcatgac gcggcgagcc atgcggcggc gcgcgaagtg aacgcgcgcg
     3901 cgttcaccct cggccagcat gtccatttcg ccgccggcga gtaccggccg gggcaccagg
     3961 acggtctgcg tcttttggcg cacgaacttg gccacactgt gcagcagcgt catgcggggg
     4021 acgtcgattc cggcacggag gtcgagatcg ggagggctga ttccgcgctc gagcgcgagg
     4081 ccgatgccgc agccgatgcc gtcctggacg gacgcaaggc gcgcctcagt tcgatcggtg
     4141 cgggcggcgt gcaggcaaaa ctgctgcagc gtcaactcgg caccgctgcg ggcggcagcg
     4201 agagcgcgag cgtggatcgc aaagtcgacg aaaacacaat cgtgcacatc acgcgcaccg
     4261 tcacagagaa aaaatgcacg ctggataccg atccggacag cacgcccacg gacaagattt
     4321 tctattggga caaggacgcc aacgccatcg gtctcaacta caagatctgc aatgggcgcg
     4381 tgcaattaag caccaagggc gagatcagct acgacaaggt gatcgattcc gccaagaatc
     4441 tgctcaccac attgcagaag aaccccgcgc tcggccagga cctcggcaat cttctgaacg
     4501 accggttgag tgccgcgacg atcagcacaa gcggcgacgt gacgctcacg gtcgatggca
     4561 tcctgtcggc ctcggtgcag accggctcca cgaccggcac acagaatcaa caatacaatg
     4621 tgcgcggagt tctgaagatc acgcccaaag gcatgagctt taccgtcacc ggtggcgtgg
     4681 atttcagcaa gacgccgttg cagagcacca ccacctacac gctggagggc aaggtcgcga
     4741 ccgagcattt cgcggtcgat ctgaaatacg aacagatcga cacctcgaag gccggagggg
     4801 cggccacaag caaggggcag acgacgctcg gcgtggatgt tcccttgccc gatgtcggcc
     4861 cgctcagtgg cgtcacattg gggccgaccg tcacgttcga tgaacatggc aatcccacca
     4921 tcggtggcgg gctgaagggg catttcggaa aaccggaaac cacgccgcgg gtgcgctgct
     4981 tcaagtgcaa ttgcccgccg ccgcttcccg aatattcctg cacgaaagag gtcaccgcgc
     5041 atcgggaacc ggtgacgaaa gaggctgcga aggacagcac cgtcaagctg ctctacgatt
     5101 acaatagcgc ggtgcccgcc aatgcggcgg acttcaaatc ccacgtcagt tccatcgccg
     5161 ctctggtggg ccagggctac aaggtcgagc acatctgggg ctatgcctcg cccgaaggct
     5221 cgctcgatac gccgaagccg ccggttccgg ggttcaaagg caatatcgcg ctgagcggcc
     5281 ggcgcgcttc gcaagcgcgc ggagagattt cgacagcggc accgggcgcc gccctgcccg
     5341 aagcggaagg caaaggcgaa caactcggcg atctcgacgg cacaggcgat accgccgaca
     5401 aggacatcac gcccgatctg gtgaaattgc tgcaatccaa aaccagcgat gacgaaaggc
     5461 tggatgtgct gggcgtcgag gaaagcgtgc gcaacgatgc ggcaaagaaa gctcaggcga
     5521 tagccgacat caacgccttc atcgaaggcc gcgatgcgaa ggggctgaag ctggcgcaac
     5581 ggccacgctg gggaaaagtg ttcccattcc tgcgccgcgt cgaggtggca cttcaccacg
     5641 atccggtgat cgaaatgaat ccggtcgcgg caagcaacaa gaaacagaat tgcgaagacg
     5701 cggacatcgc ctatgcgcgc gccaatatgc cggcgctgcc gcccgaacgc cgtctgccga
     5761 aggaagagtg cggaacatga gtttcgacat atcgccgaag atcatcaaag gcggcctcgt
     5821 ggtgctggcg ccgggcggcg gcgccgtgca acgcacgatc gcattgcaat acaatccgga
     5881 cacgctgacg cgcagctatc aggtgcaagg tgtgggcggc gacggcgggg gcgaacgcgc
     5941 gcagcctttc cgtctgaagg gtccggcgat cgaatcgatc aagcttgaag ccgatatcga
     6001 cgcgaccgag caattggagt tgcccgacca gaaccccaat gcggtggctt ttggcatcgc
     6061 accgcagctt gcgatcctcg aagccctcgt caatcccagc gtcgacgagc ttttgtccaa
     6121 cgccgcgcag agcgcgaacg gcacactcga gatcattccg cccgaagcgc cgctggtgat
     6181 cttcgtcttc agcaagaacc gcgtcgtacc ggtgcgggtg acggatttct ccatcacgga
     6241 agaggcgttc gacgtccagc tgaatcctat ccgcgccaag gtcaatctcg gcttgcgcgt
     6301 aatgagcacc gacgatctcg gcttccagca caagggcggc acgatcttcc tcggctattt
     6361 gcgcgcgcgt gaggcgctgg ccgcgaaatc cggcaccgcg aatttgagta cgctcggcat
     6421 taccaaccta gcctgaaccg gataaagcga tgacaaccga tcccgtccag ttgctgatcg
     6481 aagccggcgc cattccgtcg agcccgttcg ctgcgaacag ccggtattac agcgtcgtgc
     6541 ttgggcgcta tcagcgcgat gccaacgatg cgggcatcac ctatgtgctg cgccgcttca
     6601 ttccgcaaca gcgcgacatt cccatcgcgg gcgcgcacat cgtgcatggc ggggagcgtc
     6661 cagacctgct cgccgcgcag gccttgggcg atgccgggct ttactggcgc gttgccgatg
     6721 ccaatgccgt caccgatcca ttcgaactca ccgatacgct aggcgcgcgt atcgccattc
     6781 ctactccacc cggaacataa gcgagcggca cattatgaac ggcggcgttc agctctcttt
     6841 gcaaattggc ccggtgccca tccccgcgcc gcgagaggtc gtggacgctc tggtgcatgt
     6901 caaagtcgaa gacggctcgg gcggcacgca aagcggcttc gagctcggct tcgagctgcc
     6961 gattcgctcg cccttgcgca ccctgttcct gctgaccggc ggcggcagcc tgccgctgat
     7021 gcgcgtggtg ctcattgtca cgatcaatgg cgcggcgcgc tccatcatcg acggcgtgac
     7081 cacccatgtg gagacccagc cgggcgaagg cggcgtcgcc aagctgatcg tgaagggcaa
     7141 ggacctttcc gcgttgatgg atctcatcga gattccgggg ctgcccttcc cggccatgcc
     7201 gccttcggcg cgcgtattgc tgatcctggc gaaatatgcc gcgttcggcg tgatccccat
     7261 ggtgatcccg agcattctcg atattccacc gattcccgtg cagcagatcc cgcaacagcg
     7321 cggcagtgat tatgcctatg tgacgaagct cgcgaaggat gcgggctatg tcttttatct
     7381 ggaaccggga cccgcacccg gaacgtccaa ggcgtattgg gggccggaaa tccgcgtcgg
     7441 cgaaccgcag cccgcgctga ccaccaacat ggacgcgctc agcaatgtcg atcagatttc
     7501 gttcaatttc gacaaggaaa aaaagaccat ccccatcgtc ttcttccagg agccggtgag
     7561 caaaattccg atcggcattc ctattcccga cgtcacgccg ctcaatccac cgctcggcct
     7621 ggtgccgccg ctcccgccga agatcgtgaa gctcgacagc actgcgcatt tgtccgcgcc
     7681 cgacgcgttg atggcggggc tcgcctatgc cggacagcac agcgattcgg tgttcggcaa
     7741 gggaa
//
LOCUS       DASYEG010000217         3191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4566068, whole genome shotgun sequence.
ACCESSION   DASYEG010000217 DASYEG010000000
VERSION     DASYEG010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4566068"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..966)
                     /gene="dmeF"
                     /locus_tag="VGM72_09660"
     CDS             complement(7..966)
                     /gene="dmeF"
                     /locus_tag="VGM72_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HEY4265573.1"
                     /translation="MSERHDHAWTQGHVYLGAGHARAESRTRLVVIVTIIFMTVEIAA
                     GLVFGSMALLADGAHMATHVGALGLAAGAYWMARRHAADRRFSFGSGKFGDLAGFSSA
                     LVLGIISLGIAVESAMRLFAPVPIRYNEAILVAGLGLLVNILSAFMLADHDHDHGHDH
                     GHDHDHGQEEANHQDYNMRAAYVHILADAATSVLALLALGAGALFGLSALDPVVGIIG
                     AVVIASWAVQLMRQAGLVLLDVEDDPSLSEAIRRTLEDDLHARIADLHLWRLGPGHRG
                     LIVSLVSPVPCSADDIKAELRRRHSGLSHVTIEVSVCQDCAPA"
     gene            complement(1000..1506)
                     /locus_tag="VGM72_09665"
     CDS             complement(1000..1506)
                     /locus_tag="VGM72_09665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4265574.1"
                     /translation="MGLTIARAGPEAVAETDVLIREAADWLAAKGEPLWGPNETSYDA
                     LVEVARAGELVTGRVVGALAACMYLHDEDRLFWPRAKPEEAFYIHRLAVARKYAGQGF
                     AQAMLNWAEAEACAKARPYLRLDCEPRPKLLVLYRDAGFTRIDVKPIQVAEHFVVRHE
                     KPILPRRT"
     gene            complement(1521..3014)
                     /locus_tag="VGM72_09670"
     CDS             complement(1521..3014)
                     /locus_tag="VGM72_09670"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY4265575.1"
                     /translation="MKTLTHFIGGVHVNGSSGRFGDVFDPATGEVQARVPFANSEELR
                     RAVDAAAKAFPAWAGLNPQRRARVMFNFKTLVEESMDSLSQLLSSEHGKVVADSRGDL
                     QRGLEVIEFACGIPHLLKGEFTEGAGPGIDVYSIRQPLGVVAGITPFNFPAMIPMWMF
                     GVAIACGNCFILKPSEKDPSIPLRLAELMLEAGAPPGVLQVVNGDKEIVDAILDEPGI
                     AAVSFVGSSTIAEYVYAKGAATGKRVQAMGGAKNHAIVLPDADLDSVVNELMGAGFGS
                     AGERCMSISVVVPVTERVAESLTHRLKRRVEGLKVGLPTDPDAAYGPLITAAHRQKVL
                     DYIALGENEGAELVVDGRDFTMQGYENGFFLGASLFDRVTPGMRTYREEIFGPVLQIA
                     RARDFDEALALPSRHQYGNGVAIFTRDGDAAREFASRVNVGMVGINVPIPVPLAYHSF
                     GGWKRSAFGDANQHGPDGVRFFTKTKTVTARWPKGARDSSFVMPTMR"
BASE COUNT          632 a         1025 c         1001 g          533 t
ORIGIN      
        1 aggcgttcaa gccggggcgc agtcctggca gaccgagact tcgatcgtga cgtgggaaag
       61 tcccgaatgg cgccggcgca gttccgcctt gatgtcgtcc gccgagcagg ggaccgggct
      121 caccagcgat acgatcagac cgcggtggcc ggggcccagc cgccagaggt gcaaatcggc
      181 gatgcgcgcg tgcaaatcgt cttcaagcgt gcggcggatc gcctccgaga gcgagggatc
      241 gtcctcaaca tcgagaagca caagacccgc ctgccgcatg agttgaaccg cccagctcgc
      301 aatcaccacg gcgccgataa ttcccaccac cggatcgagc gcggacaaac cgaacagggc
      361 ccccgcgccg agcgccagca atgccagcac ggatgtcgcg gcgtccgcca atatgtgcac
      421 ataagccgcg cgcatattgt agtcctgatg gttcgcctcc tcctgcccgt gatcatggtc
      481 gtgcccatga tcgtgcccgt gatcgtgatc gtggtccgcc agcatgaacg cgctcaggat
      541 attcaccaga agccccagcc ccgcgaccag gatggcttcg ttgtagcgaa tgggaacggg
      601 ggcgaagagc cgcatcgccg attccacggc aatgccgagc gaaatgatcc ccagcacgag
      661 cgcgctggaa aatcccgcga gatccccgaa tttaccggaa ccgaaagaaa agcgccggtc
      721 ggccgcgtgg cggcgcgcca tccaataggc gcctgccgcg agaccgagcg cgcccacatg
      781 ggtggccata tgcgcgccat cggcgagcaa cgccatggag ccaaagacga gacccgcggc
      841 gatctccaca gtcatgaaga taatcgtgac aatgacgacc aaacgggtgc gggattcggc
      901 gcgggcgtgg ccggcgccga gatagacatg cccctgcgtc caggcgtgat cgtgccgttc
      961 gctcatgagg ccaatatagg tgtgaagagg ccgttacgac tatgtgcggc gcggtaagat
     1021 aggtttttcg tgccgcacca cgaaatgctc cgccacctgg atcggcttga cgtcgatacg
     1081 ggtgaagccc gcatcgcgat aaaggacgag caatttcggc ctcggctcgc aatcgagcct
     1141 gagataagga cgtgctttcg cgcatgcttc agcttcggcc cagttcagca tcgcctgcgc
     1201 gaagccttgg ccggcgtact tacgtgcgac agcaaggcgg tggatgtaga aggcttcctc
     1261 gggtttggcc cggggccaga agagtctatc ctcgtcgtgc agatacatgc aggcggcaag
     1321 cgcccccacg acgcgtccgg tgacaagctc gccagcgcgc gcaacctcca ccaacgcatc
     1381 gtaagaggtc tcgttcggtc cccagagcgg ttcgcccttg gcggccagcc agtccgccgc
     1441 ctcgcggatc agaacatcgg tctccgcaac ggcctcggga ccggcacgcg cgattgtgag
     1501 gcccatcgcg gatgacgcgc ctagcgcatc gtcggcatga cgaagctcga atcgcgcgcg
     1561 cccttgggcc agcgcgccgt caccgtcttc gtcttggtga agaagcgcac gccgtccggc
     1621 ccgtgctggt tcgcatcgcc gaaggccgaa cgcttccagc cgccgaaact gtgataggca
     1681 agcggtacgg gaatcggcac gttgatgccg accatgccga cattcacgcg cgatgcgaat
     1741 tcgcgcgcgg catcgccgtc acgcgtgaag atggcgacgc cattgccata ttgatgccgc
     1801 gagggcagcg ccagggcctc gtcgaaatcc cgcgcgcgtg cgatctggag cacgggaccg
     1861 aaaatttcct cccgataggt tctcatgccg ggcgttacgc ggtcgaacaa cgaagcaccg
     1921 aggaagaagc cgttctcgta accttgcatg gtgaagtcgc gcccatcgac gacgagttcg
     1981 gcgccctcgt tctcaccgag tgcgatgtag tccagcacct tctggcgatg ggccgccgtg
     2041 atgagcgggc cataggcggc gtcgggatcg gtgggaagac cgacctttaa cccttcaacg
     2101 cgccgcttga gtctgtgcgt gagggattcg gccacgcgtt cggtgaccgg cacgacgacg
     2161 gaaatcgaca tgcagcgctc gcccgccgaa ccgaagcccg cgcccatcag ctcgttcacc
     2221 accgaatcga gatcggcatc gggcagcacg atggcgtggt tcttggcgcc gcccatcgcc
     2281 tgcacgcgct ttccggtcgc ggcgcctttc gcatagacat attcagcgat tgtcgatgaa
     2341 ccgacaaaac tgaccgcggc gatgcccggc tcgtcgagga tggcatcgac gatctccttg
     2401 tcgccgttca ccacctgaag cacgccgggc ggcgcgcccg cttccagcat cagctccgcc
     2461 agacgcaagg ggatgctcgg gtctttctcg cttggcttca gaataaagca attgccgcag
     2521 gcgatggcga caccgaacat ccacatcgga atcatggcgg ggaaattgaa cggcgtgatg
     2581 ccggcgacga cgcccaaggg ctggcgaatg gaatagacgt cgatcccggg gccggcgcct
     2641 tcggtgaatt cgcctttcaa cagatgcggg atgccgcagg caaattcgat cacttcgagg
     2701 ccgcgctgga ggtcgccgcg cgagtcggca acgaccttgc catgctcgct ggaaagaagc
     2761 tggctcagcg aatccatact ttcttcgacc agcgtcttga aattgaacat cacccgcgcg
     2821 cggcgctgcg ggttgaggcc ggcccatgcg gggaacgcct tggcggcggc gtccaccgcg
     2881 cggcgcagtt cttcggaatt ggcaaagggc acacgcgcct gcacctcgcc ggtcgcggga
     2941 tcgaacacat cgccgaagcg gcccgacgag ccgttcacat gcacgccgcc gatgaaatgc
     3001 gtcagggttt tcatgaaagc ctcaccgggt tctggcgaag catagcgcac cgtgcgaaat
     3061 gtcgaaacgc gtcagccgag cgctttgacg atttcttccg tcatcttctt cgcatcggcg
     3121 aacagcatca tggtgttgtc gcggaagaac agctcgttct cgataccggc atagcccgag
     3181 cccatgccgc g
//
LOCUS       DASYEG010000218         8280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2390396, whole genome shotgun sequence.
ACCESSION   DASYEG010000218 DASYEG010000000
VERSION     DASYEG010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 8280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8280
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2390396"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VGM72_09675"
     CDS             <1..996
                     /locus_tag="VGM72_09675"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4265576.1"
                     /translation="PSGVVLTGEYFPLARRSSSITFIYCGFSFGQGSAGEVANAVLQP
                     YGWQAAILIGGGLALALAATLAVLLPESLEYLVNRGGNPQTAAKILRRIAPDIAIGPE
                     TRLVAGARNGATLTFWQLFPQLFQMGRSFGTLVIWLALGMNLIVNTSLQVWLTNILQD
                     SGFAQHTAIIATEASFAAGIVGAFIIGPLIDRFGPYRVMGALFVVGALFSLFIGLSLS
                     WAVAATIIAASFGSGFCTSGVQKGGNAMSVYFYPVALRSTGLGWGLGIGRIGAILGPL
                     AVGYLLTSGWPAPTVFAIMAVPMLIGASAIFAMGRAYGEAQKEQPAVQEIAATKA"
     gene            1006..2379
                     /locus_tag="VGM72_09680"
     CDS             1006..2379
                     /locus_tag="VGM72_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4265577.1"
                     /translation="MDSPAAVETDALINESRVGRLQILVAVLGGCALLVEGFDTSVIG
                     YIAPQLTRAWHVPPSTLGTILTADMVGLLLGYLFVSPLSARFGHKRMVVFSTAAFGAL
                     TFLTITSGDVAMLIGFRFLTGIGVGGAMPSAVALTGEYFPERMRSTSVTLIYIGFSLG
                     QIAAGVVANLLLDSFGWQAVLAFGGAGTLLLSALFLFMLPESLEYLINRGDDRARAIT
                     ILARLAPGLTISRDTRLIAGQQGARHVAVGQLVEDGRALGTGLIWIGMFMNLMVYFFL
                     QKWLTQLLVNVGLPQQTAITATTVGLAGGIVAAIIIGPLMDRIGPYIVVAGLFAMAAV
                     SVAVMGEVLASPAPVVIVAVSLFMGFCLSGGQKANNALSVYFYPTALRGTGLGWSLGV
                     GRIGGVIGPFAAGILLTAGWSPAELFYASALPMIAGAIAIAMMGQFYGHAGTASPVSQ
                     KISPHKV"
     gene            2386..3333
                     /gene="glk"
                     /locus_tag="VGM72_09685"
     CDS             2386..3333
                     /gene="glk"
                     /locus_tag="VGM72_09685"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEY4265578.1"
                     /translation="MTSSPRLLADIGATHARFALLIDGRIGHNFVHMVADYANFADAI
                     AAYLASIPENLWPVQAAMAVAGPVTSDKVVMMNSPWNFSIAGLKQQFGWTRLHVMNDF
                     AANALAVPHLAEDDLIQVGEGAPVPDAPVAVLGPGTGLGVAGLVRCAESWIALPGEGG
                     NITLAALDSREAAIVDILRRQYAHVSAEIVLSGPGLVNLYDSLCELSGKPATPSTPEH
                     ITHVYPGCDPQCREAVNVFCAMLGTFAGDCALTFGARGGVYIMGGIVPKMIEVFRRSA
                     FRERFEFKGRYRQYLSTVPSYVVMHPFPAYLGLRSLFED"
     gene            3336..4415
                     /locus_tag="VGM72_09690"
     CDS             3336..4415
                     /locus_tag="VGM72_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265579.1"
                     /translation="MSAMTRAAFLRCAGASLAYGAAGALARPALAQNAEAFYRGRTVT
                     LLIPTAPGGINNLSARLVARHLGRFIPGNPNIVAENRAAGGGLALANSFAVNPSTNAA
                     PKDGATIAILQRGIAQLAIQGDKTAKFDPLALTWLGSLSSFATDAYILIVNARHPAKT
                     VKDLAPPAPPALIGGDDPGSTNLTFALVARDALKLNIEVKDGFAGAPGMFFAMMQGDL
                     DGQVIGLNSIRANQAALWNAKEVRVLLQFGRATRHPLLPDVPLASELTRDPAALSVIA
                     FTEAPLYMALPFLAPAGIPPDRAAALRSGFMAMVKDKDFLADAAKAKLDITPIDGDSV
                     RDIIVKMAATPKDVIARYNKITGVT"
     gene            4751..5074
                     /locus_tag="VGM72_09695"
     CDS             4751..5074
                     /locus_tag="VGM72_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265580.1"
                     /translation="MKRVAKVVFWIYAAFFPIGMIGLFVYGYCAMSASGFHSTPIPDS
                     ASGHVFPFVVGPRGGPARTIYVTRAYSLTAEIGWDLFLAWWIGTICMIAAGAAYRFRQ
                     RQISN"
     gene            complement(5109..5462)
                     /locus_tag="VGM72_09700"
     CDS             complement(5109..5462)
                     /locus_tag="VGM72_09700"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4265581.1"
                     /translation="MAVQLNHTIVWCRDKKKSAKFLQEILGLPKSARFGPFQVVTLSN
                     GVSLDFYETEDEIASQHYAFLIGDEEFDPIFGRIQERGLEYWADPGKSHAGKLNHRDG
                     GRSLYFEDPNGHFLY"
     gene            complement(5474..6217)
                     /locus_tag="VGM72_09705"
     CDS             complement(5474..6217)
                     /locus_tag="VGM72_09705"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEY4265582.1"
                     /translation="MPYESIIPRARIGFIIPASNRMVEPQMARFAPPGVVPHFMRLRM
                     TNRYKRPLPELLPAILDAAAHLMDSKCDIIVFQCTGTSMSGGVDMDHRVVAEIARATK
                     RPAISTAGAIKAALAALDARRLVFISETEQTGHDKKVKFLKEAGYELLADKAAALAGS
                     DSYCITPPRFWYDLAISLRNDAADAVFISCANISSIEMIGELEAALKKPVVTSNQAAL
                     WCALRTLGISDNVPGLGALFQRELAKIAA"
     gene            complement(6218..6775)
                     /locus_tag="VGM72_09710"
     CDS             complement(6218..6775)
                     /locus_tag="VGM72_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265583.1"
                     /translation="MRVAIFWGLIGIVTALVALPAEAAWHGYVNVEAGFSFTAPGDVK
                     VEKAAYTSKLEGARTATVFRSVEDNVEYRVTVINFFGRKNDEPALIKEASAMFVADKK
                     VLADTEARVESSYGRKITVDLPNNGGRAMGAIYFNQGYLIELQVTILPANGDYGSPDM
                     GRFVDSIAFGPDRAQEGATDLKLQR"
     gene            complement(6867..7709)
                     /locus_tag="VGM72_09715"
     CDS             complement(6867..7709)
                     /locus_tag="VGM72_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4265584.1"
                     /translation="MTVTPAGDVSIQAREDADLAAPTVSRWRRNENFVLGTASVVAFL
                     LFWEFSVVFGWVNPLFTSSPSRIFAAAFEMFRDGSIYPDLAVSAEEFVLGYGLAVVVG
                     VPVGILMGWYSRLNAVLDPFVNALYATPRIALMPLIIIWFGIDLSAKIAIIFLSTVFP
                     ILVNTMTGVRTMERDFVKVARSFGASDAQLFKTVALPSSVPNLLTGLRLGLGHALIGI
                     VVGEMYGASAGIGFMMQTAGATFQTDKVMVGILIIAAAGMGMTQGLRMIEKKFDVWRP
                     DHHV"
     gene            complement(7714..>8280)
                     /locus_tag="VGM72_09720"
     CDS             complement(7714..>8280)
                     /locus_tag="VGM72_09720"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4265585.1"
                     /translation="GPDRAMVFQQDSLFPWRTVLGNVTYGLELQKRLPKEEMRARAAA
                     LVDLVGLTGFGDHYPHELSGGMRQRVNIARALAPDPELLLLDEPFAALDAQTREFMQS
                     ELLKILARAGKTALFITHQIDEAIFLSQRVVVFSARPARVKATIAVDLPGTRSLEVKL
                     QPRFRELQDHIWRLIQEESVRAPAEMAG"
BASE COUNT         1480 a         2645 c         2520 g         1635 t
ORIGIN      
        1 ccttcgggcg tggtgctgac cggcgaatat tttccgctgg cccgccgctc cagttccatc
       61 acctttatct attgcgggtt ttcgtttggc caaggctccg ccggcgaagt cgcgaatgcg
      121 gtgctgcaac cctatggctg gcaggcggcg atcctgatcg gcggcggtct cgcccttgcg
      181 cttgcggcga cgctggcggt gcttctgccc gaatcgctcg aatatctcgt caatcgcggc
      241 ggaaatccgc aaaccgcggc caaaattctc cgtcgcatcg cgcccgatat cgccatcggt
      301 ccggaaaccc ggctggtcgc gggagcgcgc aatggcgcca cgctgacctt ctggcaattg
      361 tttccgcagc ttttccagat ggggcgcagc ttcggcacac tggtgatctg gctggcgctc
      421 ggcatgaacc tgatcgtcaa cacatcgctg caggtgtggc tgaccaatat cctccaggat
      481 tccggcttcg cccagcacac cgccatcatc gcgaccgagg cgtccttcgc cgcaggcatc
      541 gtcggggcct tcatcatcgg accgctgatc gacagattcg gaccttaccg cgtcatgggc
      601 gcgttgttcg tcgtgggcgc attgttcagc ctcttcatcg gcctgtcgct ctcctgggcc
      661 gtggcggcca cgatcatcgc ggcctcgttc ggctcgggct tctgcaccag cggcgtgcaa
      721 aaaggcggca acgcgatgtc ggtctatttc tatccggtgg cgctgcgttc cacaggcttg
      781 ggctggggcc tcggcatcgg gcgcatcggt gcgattctgg gaccgctggc ggtcggatat
      841 ctgctgacat ccggttggcc ggccccaacc gtgttcgcaa taatggccgt gccgatgctg
      901 atcggggcaa gcgccatctt cgccatggga cgcgcctatg gcgaagccca aaaagaacag
      961 ccggccgtcc aagaaatcgc cgcgaccaag gcctagagga gcagcatgga tagcccggcc
     1021 gcggtcgaga ccgatgcgct gatcaatgaa agccgggtgg gacggctgca gattctcgtc
     1081 gccgtcctcg gcggctgcgc gcttctggtc gaaggcttcg atacctccgt catcggctat
     1141 atcgcaccgc agctcacgcg ggcgtggcac gtgccgccga gcacgcttgg caccatcctg
     1201 accgccgaca tggtggggct gctgctcggc tatctgttcg tctcgccgtt gtccgcgcgg
     1261 ttcggccaca agcgcatggt cgtgttcagc acggcggcat tcggcgcgtt gacgttcctc
     1321 accatcacgt ccggcgatgt cgcgatgctg atcggctttc gcttcctgac cggcatcggc
     1381 gtcggcggcg cgatgccgag tgcggtggcg ctgaccgggg aatatttccc cgagcgcatg
     1441 cgctcgacct cggtcacgct catctatatc ggcttctctt tgggacagat cgcggccgga
     1501 gtggtggcga accttctgct cgattctttc ggatggcagg ccgtgctcgc cttcggcgga
     1561 gccggcacgc tgctgttgtc ggcgctgttc ctgttcatgc ttcccgaatc gctcgaatat
     1621 ctcattaatc ggggggatga ccgcgcgcgg gcgatcacga tcttggcccg tctcgctccg
     1681 ggattgacga tttcgcgcga cacgcggctg atcgccggcc agcagggcgc gcgacacgtt
     1741 gcggtcggtc agttggtgga agacggacgc gccctcggca ccgggctgat ctggatcggc
     1801 atgttcatga acctgatggt ctatttcttc ctgcagaaat ggctgaccca attgctggtc
     1861 aatgtggggc tgccgcaaca gaccgccatc accgcgacga ccgttggctt ggcgggcggg
     1921 atcgtagcgg caatcatcat aggcccgctg atggaccgga tcggtcccta catcgtcgtt
     1981 gcaggccttt tcgcgatggc ggcggtctcc gtcgccgtca tgggagaggt gctggcctcg
     2041 cccgcacccg tcgtgatcgt ggcggtgtcg ctgttcatgg gtttctgtct ttccggcgga
     2101 caaaaggcca acaatgcgct ctcggtctat ttctatccga cggcgctgcg gggcacgggt
     2161 cttggctggt cgctcggagt gggacgcatc ggcggcgtca tcgggccgtt tgccgcaggc
     2221 attctcttga cggcggggtg gtctccggca gaacttttct atgcctcggc gctgcccatg
     2281 atcgcaggcg ccattgccat cgccatgatg ggacagttct atggacatgc gggaacggca
     2341 tcgccagtgt cccaaaaaat ttcaccgcat aaggtctgat tcacagtgac atcatccccc
     2401 cgcctcctcg ccgatatcgg cgcgacgcat gcgcgcttcg cgcttctgat cgatggccgg
     2461 atcggccaca acttcgtgca catggtggcc gattacgcca atttcgcgga cgcgatcgcc
     2521 gcttatctcg cctccatccc ggaaaatctg tggcccgtgc aagccgcgat ggcggtggca
     2581 gggccggtca cgagcgacaa ggtcgtcatg atgaacagtc cgtggaattt ttccatcgcc
     2641 ggactgaagc agcaattcgg ctggacccgg ctgcatgtga tgaacgattt cgcggccaat
     2701 gcgctggccg tgcctcatct ggccgaggac gatctgatcc aggtgggcga aggtgcgccc
     2761 gtgcccgatg cccccgtggc cgttctcggc cccggcacgg gtctcggcgt cgccggcctt
     2821 gtgcgctgcg ccgagagctg gatcgcgctt cccggcgaag gcggcaatat cacgctcgcg
     2881 gcgctggatt cacgcgaggc cgccatcgtc gacatcctgc gccgccaata tgcccatgtg
     2941 tcggccgaga tcgtgttgtc cggcccgggt ctggtgaacc tctacgattc cttgtgcgaa
     3001 ctttccggca agcccgcgac accttcgacg cccgagcaca tcacccatgt ctatcccggc
     3061 tgcgacccgc aatgccgcga agcggtcaat gttttctgcg ccatgctggg cacgttcgcg
     3121 ggggattgcg cgctgacctt cggcgcgcgc ggcggtgtct acatcatggg cggcatcgtg
     3181 ccgaagatga tcgaggtttt ccggcgttcg gcgttccgcg aacgtttcga gttcaagggc
     3241 cgctaccggc aatatctctc caccgtgccg agctatgtgg tgatgcatcc cttccccgcc
     3301 tatctcggtc tgcggagcct gttcgaggat taggcatgag cgcgatgacg cgggcggcat
     3361 tcttgcgatg cgcgggcgcg agcctggcgt acggcgccgc gggagcgctg gcgcggcccg
     3421 cgttggcgca aaatgcggaa gccttctatc gcggccgaac ggtaacgctt ctcatcccca
     3481 ccgcgcccgg cggcatcaac aatctgtcgg cgcggcttgt ggcgcgtcat ctgggccgct
     3541 tcattccggg aaatccgaac atcgtcgcgg agaatcgcgc ggccggcggc gggctcgctc
     3601 tcgccaacag cttcgcggtc aacccctcca caaacgcagc gccgaaagac ggcgccacca
     3661 ttgcgattct gcagcgcggc atcgcgcagc tcgcgatcca gggcgacaaa accgcgaaat
     3721 ttgaccctct ggcgctaacc tggctcggca gcctctcgtc cttcgcgacg gatgcctaca
     3781 tcctcatcgt caatgcgcgc catcccgcca agacggtgaa ggatctggcg ccgcccgcac
     3841 cccctgcgct catcggcggc gatgatcccg gctcgaccaa tctgacattc gccctcgtcg
     3901 cgcgcgacgc cctcaagctc aatatcgagg tgaaggacgg cttcgccggt gcgccgggca
     3961 tgttcttcgc catgatgcag ggcgatctgg atgggcaggt gatcggcctg aattcgattc
     4021 gcgccaatca ggcggcgctg tggaacgcga aagaggtgcg tgtgctgctg caattcggac
     4081 gcgccacccg ccatccactc ctgcccgacg tgccgctcgc aagcgagctg acgcgcgacc
     4141 ccgcggcgct gtcggtgatc gccttcaccg aggcgccgct ttatatggcg ctgccgtttc
     4201 tggcgccggc cggcataccg cctgaccgcg cagccgcgtt gcgctccggc ttcatggcga
     4261 tggtgaagga caaggatttt ctcgccgacg ccgcgaaggc gaaactcgac atcacgccca
     4321 tagacggcga ttcggtgcgc gacatcatcg tcaaaatggc cgcgacaccg aaggacgtca
     4381 tcgcgcgcta caacaaaatc accggcgtga cttgattatt ttgcactgtc tcagtttgaa
     4441 ttcacgcggc taacggcggg ccactctaag aaattcgaga accattgcca cgcgctagcg
     4501 gtctactttg ccttctataa ttttgtgaag gcgcattcgt ccttgcgcat gtcgcccgct
     4561 atggccgcag gcatcgagac aaagatttgg gaaatgaagg accttgtgga gtatattgat
     4621 agccgcgaac cgccagctaa aaaatgtggt ccctacaaga agaaggcggc ataaagcagg
     4681 ccttgggctc ccttgggcat ggacgtaata tggtatgggt cggggctgaa cgggaggggc
     4741 gtccgaacag atgaaacgtg tcgcgaaggt ggtcttctgg atttacgccg ccttctttcc
     4801 aatcggtatg atcggcctct ttgtgtatgg atactgcgcc atgtcggcgt cagggtttca
     4861 cagcactccg atccccgatt cagcgtcggg gcacgttttt ccttttgttg tcggtccacg
     4921 cgggggcccg gcgcgaacga tctatgtgac gcgtgcctat agcctcactg ctgaaatcgg
     4981 ctgggatttg tttctagctt ggtggattgg cacgatttgc atgattgcag cgggagccgc
     5041 ttaccgattc cgccaacgcc aaatttcaaa ctgagacagt gccgattatt cgctgccgta
     5101 aggacgggtc agtagagaaa atggccgttc gggtcttcga aatacaggct acgaccgcca
     5161 tccctgtggt tgagcttacc cgcgtggctc ttgcccggat cggcccaata ttccaacccg
     5221 cgctcctgaa tgcgtccaaa tatcggatcg aactcctcat cgccgatcag gaacgcgtag
     5281 tgctgggatg cgatctcgtc ttcggtctcg tagaaatcga ggctgacacc attgctcaat
     5341 gtgaccactt ggaacgggcc gaaccgcgca gacttcggaa ggccgagaat ctcctgaagg
     5401 aacttcgccg atttcttctt gtcgcggcac cacacgatcg tgtggttgag ttgcacagcc
     5461 attgtttgcg atcctatgct gcgatcttcg cgagctcgcg ttggaacagc gcgccgaggc
     5521 ccggcacgtt gtcgcttatc ccgagcgtgc gcagcgcgca ccagagggcc gcctgattgg
     5581 acgtgacgac gggcttcttc aacgcagctt ccaattcgcc gatcatctcg atgctgctga
     5641 tattggcgca ggagatgaaa accgcgtccg ccgcatcgtt gcgcagcgaa atcgccaaat
     5701 cgtaccagaa gcgcggcggc gtgatgcaat aggaatccga gccggcgagc gccgcggcct
     5761 tatcggccag caattcgtat cccgcctctt tcagaaattt caccttcttg tcgtgccccg
     5821 tctgttcggt ctccgaaatg aataccagcc gccgcgcgtc caatgcggca agtgccgcct
     5881 tgatcgcgcc cgccgtgctg atggcgggac gcttcgtcgc gcgcgcgatc tccgccacga
     5941 cgcgatggtc catgtccacg ccgcccgaca tcgacgtccc ggtgcactgg aacacgatga
     6001 tgtcgcattt ggaatccatc agatgcgcgg ccgcatcgag gatcgccggc agcaattcgg
     6061 gcagcggccg cttgtagcga ttggtcatgc gcaggcgcat gaaatgcggg accacgcccg
     6121 gcggcgcaaa acgcgccatc tgcggctcca ccatgcggtt ggacgcggga atgatgaagc
     6181 cgatccgcgc gcgcggaata atcgattcgt atggcatcta tctctgaagc ttgaggtcgg
     6241 tcgcgccctc ctgggcccgg tccggaccga aggcaatcga atcgacaaag cgtcccatgt
     6301 cgggcgagcc gtaatcgcca ttggccggaa ggatggtcac ctgaagttcg atcagatagc
     6361 cctggttgaa atagatcgca cccatcgccc ggccgccatt gttgggaaga tcgaccgtta
     6421 ttttgcggcc atagctcgat tcgacccgcg cttcggtgtc cgccaagacc tttttgtcgg
     6481 cgacgaacat ggccgacgct tccttgatga gcgccggttc gtcgttcttg cggccgaaaa
     6541 aattgatcac cgtcaccctg tattcgacat tgtcctcgac agaacggaag accgtggcgg
     6601 tgcgcgcgcc ttcgagcttg cttgtgtacg ccgctttttc gaccttcacg tcacccggtg
     6661 cggtgaagga aaacccggcc tccacattca catagccatg ccaggccgcc tcggcgggaa
     6721 gagccaccaa cgccgtgaca atcccgatca atccccaaaa aatcgcgacg cgcatcgagc
     6781 cctccatttg tgcaagccag gcgatcccac agccgcggct ctcccgagac tatacgaaac
     6841 tcgccgccat aaagcatcgg ccggctctat acgtggtgat ccgggcgcca gacatcgaat
     6901 ttcttttcga tcatccgcaa gccctgcgtc atccccatgc cggccgcggc gatgatgagg
     6961 atgcccacca tcaccttgtc ggtctggaag gtggcgcccg ccgtctgcat catgaagccg
     7021 atccccgccg aggcgccgta catctcgccc accacgatgc cgatcagcgc gtggcccaat
     7081 cccagcctga gacccgtcaa gagattgggc accgacgacg gcagcgccac ggtcttgaac
     7141 aattgcgcgt cggacgcgcc gaaggagcgc gccaccttga cgaaatcgcg ttccatggtg
     7201 cgcacccccg tcatggtgtt gaccaggatg ggaaacacgg tggagaggaa gatgatcgcg
     7261 atcttggccg acaggtcgat gccgaaccag atgatgatca gcggcatcag cgcgatgcgc
     7321 ggcgtcgcat aaagcgcatt cacgaacgga tcgagcaccg cgttcagccg tgaataccag
     7381 cccatcagta tccccaccgg cacgccgacg acgacggcca gcccatagcc gagtacgaat
     7441 tcctccgcgc tgacggcgag atcgggatag atgctgccgt cgcggaacat ctcgaaggcg
     7501 gccgcgaaga tacggctggg cgaactggtg aacagcggat tgacccagcc gaagacgacc
     7561 gagaattccc agaacagcag gaaggccacc accgaagcgg tacccagcac gaaattctcg
     7621 ttccgccgcc agcgcgagac cgtgggcgcg gcaaggtccg cgtcctcgcg cgcttggatg
     7681 gaaacatcgc cggccggggt gacggtcatc gcctcaaccc gccatctctg ccggcgcgcg
     7741 caccgattct tcctggatca ggcgccagat atgatcctga agttcgcgga atcgcggctg
     7801 aagcttcacc tcgagactgc gcgtccccgg cagatcgacg gcgatcgtcg ccttgacgcg
     7861 cgcgggccgc gccgaaaaca ccaccacgcg ctggctgagg aagatcgcct cgtcgatctg
     7921 gtgggtgatg aacaaagcgg tcttcccggc gcgcgcgaga atcttcaaaa gctcgctctg
     7981 catgaattcg cgcgtctgcg cgtccagcgc ggcaaagggc tcgtccagca gcagcaattc
     8041 cggatcgggc gcgagcgcgc gcgcgatgtt cacccgctgg cgcatgcctc ccgaaagctc
     8101 atgcggataa tgatcgccaa aaccggtcag cccgacgaga tcgaccaacg ctgccgcccg
     8161 cgcgcgcatc tcctctttcg gcaggcgctt ctgcagttcc aggccgtacg tcacattgcc
     8221 gagcaccgtg cgccagggaa aaagcgaatc ctgctggaac accatggcgc gatccggacc
//
LOCUS       DASYEG010000219         3422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4422262, whole genome shotgun sequence.
ACCESSION   DASYEG010000219 DASYEG010000000
VERSION     DASYEG010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4422262"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VGM72_09725"
     CDS             <1..811
                     /locus_tag="VGM72_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265586.1"
                     /translation="GMGTRQSAGRGWLVLVSALFCTLAYNLTFFVQELMLTLPKAFVP
                     GVHVWLYHNNHHYEGNDPILPLLQGTGGVADLAVGLIFAAFLSGSGGRSMTARLFLFW
                     MAFQGLYAGLSQIVIGALIPQNDMGMAYGYLGLTQTARMAMLAFGLIASVLAGFWLAR
                     KAILLLATTGETLNAGPRMGFLFFAVLLPVLIAGALIIPFRIPRDMIEVTIFPTIVML
                     FGAIWVLIGGALSSTAPRPAHPAPSLAVPLAALIVVLLIFQLVLRPGIAFS"
     gene            complement(1028..1789)
                     /locus_tag="VGM72_09730"
     CDS             complement(1028..1789)
                     /locus_tag="VGM72_09730"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY4265587.1"
                     /translation="MDDLTARALLERHWAASDAGDFLAEHDIYRDDAVLDYPQSGERI
                     RGREHIRESRYLQPSKKRFTVRRILGGGDLWITEFTLSYDGVPSYAVSIMEFQDGLVV
                     HETQYFADGFAPAPSRAHLVEAIPARIACRAPGWCGQFEANVLRLPIRSARVALVWRD
                     AMLASAIWIASLTHPRTTYISATGDKPAPAPRQTLAAAISRRSAKILVSAGRDRLRSP
                     KATLMDGGASAACAALRVSARKAVVARMRSMAKLL"
     gene            complement(2000..2287)
                     /locus_tag="VGM72_09735"
     CDS             complement(2000..2287)
                     /locus_tag="VGM72_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY4265588.1"
                     /translation="MRDYYVYMLASKRNGTLYVGVTNDLGRRAGEHRDKVAPGFTKRY
                     GVSLLVWYEVFGDINDAIAREKQIKSWNRAWKVRLIEKGNSGWNDLAAGLV"
     gene            complement(2441..>3422)
                     /locus_tag="VGM72_09740"
     CDS             complement(2441..>3422)
                     /locus_tag="VGM72_09740"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY4265589.1"
                     /translation="GILPKRKKGDFAYWKGLVPGDTSETLWQDSDVMTFEEIPHVANP
                     SSGFVQNTNDPPWTSTWPQAIHYKDYPSYIAIEGPLSMRNEQSMHLMADSGKISYEDF
                     IRLKHTTTALLADRMVPEILKAAAGSNDAEVQKAVAVLKAWDHQYNADSKGALLFEEW
                     VKAFAGPSMSNQKNYAVKWDANDPMETPRGIADASVGVASLKTAAAETVKQYGAIDRP
                     YGEVSRFHIGDVNIPGNGGFGNLGIFRTITWDKLENGQRKVNAGETWVSMVEFSTPIK
                     AMGVMSYGESSQPGSKHNSDQLHFVAERKLRTLWVKRPDVEKHVEERTSY"
BASE COUNT          600 a         1097 c          995 g          730 t
ORIGIN      
        1 cggcatgggc acacggcaat cggcggggcg cggctggctg gtgctggtgt cggcgctgtt
       61 ttgcaccctc gcctacaatc tcaccttctt cgtgcaggag ctgatgctga ccctgcccaa
      121 ggccttcgtg ccgggggtgc atgtctggct ctatcacaac aatcatcatt atgagggcaa
      181 cgatcccatc ctgccgctgc tgcaaggcac gggcggcgtg gccgatctgg cggtgggatt
      241 gatctttgcc gcgttcctgt ccggttccgg cggccgctcg atgaccgcgc ggctgttcct
      301 attctggatg gcgtttcagg gtctttacgc ggggctcagc cagatcgtga tcggggcgct
      361 gatcccgcaa aacgatatgg gcatggcgta tggttatctg ggcctgacgc aaacggcccg
      421 gatggcgatg ctggcgttcg gcttgatcgc gtcggtgctg gcaggcttct ggctggcgcg
      481 caaagcgatc ctgctgctgg cgacgacagg cgagaccttg aatgcgggcc cgcgcatggg
      541 ctttctgttt ttcgccgtgc tgttgccggt tctgatcgcg ggcgcgctca tcattccctt
      601 ccgcataccg cgcgacatga tcgaagtgac gatctttccc accatcgtga tgctgttcgg
      661 cgcgatctgg gttctgatcg gcggcgcgct gagttcgacc gcaccgcgcc ccgcccatcc
      721 cgcaccatca ctggcggtgc cgctggcggc gttgatcgtg gtgctgctga ttttccaact
      781 cgtgcttcgg cccggcatcg cgttttcgtg accgcacgct gaccgttttt cgcgcagcgc
      841 tttcaaaagc gcgcggcaac acgcactttc ttgtgcccat cccgtgcgca atcactgcca
      901 tattgcagtg caatatattt tgttgacggc cagtaaaaac cgcgcaaact caatatcgca
      961 gtgcaacgga cgactcgggg aagttgtccg gactgcgagc aggggtgaaa cgctttaacc
     1021 gctatcgtta aaggagtttc gccatgcttc gcatcctcgc caccaccgct tttctcgcgc
     1081 tcaccctcaa cgccgcgcag gccgcagagg ctccgccgtc catcaatgtc gccttcggcg
     1141 atctcaacct gtcgcgtccc gccgatacca agattttggc cgagcgcctg gagatcgccg
     1201 ccgccaaagt ttgccgcggc gccggcgcag gcttgtcacc cgtcgccgaa atgtatgtcg
     1261 tgcgcggatg cgtcaaagac gcgatccata tcgcgctcgc cagcatcgcg tcgcgccaga
     1321 ccaacgccac acgcgccgat ctgatcggta gccgcaagac attggcttcg aactgaccgc
     1381 accatcccgg cgcgcgacac gcgatgcgcg ccgggattgc ttcgaccaga tgtgcgcgtg
     1441 acggcgcggg cgcaaagccg tcggcaaaat attgcgtctc atggacgacc agcccgtcct
     1501 ggaattccat gatgctcacg gcataggacg gcacgccgtc atagctgagg gtgaattcgg
     1561 tgatccagag atcgccgccg ccgagaatgc gacggaccgt gaagcgcttt ttgctgggct
     1621 gcagataccg gctctcgcga atatgctccc ggccccggat gcgctcgccc gattgcggat
     1681 agtccagcac ggcatcgtcg cgataaatgt catgctccgc cagaaaatcg cccgcatccg
     1741 acgccgccca atgccgctcc aataacgccc gcgcggtcag atcatccatc gcaatttcct
     1801 tctcgcggca aacctatcgt gtcattcccg gccgagcgaa gcgatgtgag gggggccaga
     1861 tatcgcaacc gtcccggtga taaccacctg gatccccttc cctcgcgcta cgcgctcgcc
     1921 gggggtgaca ttacggagtg gaattgtcat ccccggcctc acgatgcacc agcatcgtga
     1981 ggggaagggg atccaggtat caaaccaagc ccgcggccaa atcgttccag ccggaattgc
     2041 ccttctcgat taatcgaacc ttccaggcgc gattccaact cttgatctgt ttttcgcgcg
     2101 cgatggcgtc gttgatgtcg ccgaacactt cgtaccagac caacaggctc accccgtacc
     2161 gtttggtgaa cccgggcgca accttatccc tatgttcgcc agcccggcgc cccagatcat
     2221 tcgtcacgcc gacatacagc gtgccattgc gcttactggc gagcatgtac acgtaatagt
     2281 cacgcatgcg ttgacgcctg gatccccttc cctcacgcga tgctacgcat cgcgctcgcc
     2341 ggggatgaca atcttatctc ctggtgtcat tcccggccga gcgaagcgag gggaagggaa
     2401 tccaggcatc ccaaccggcc tcgctgggga agacgtgtga ctaataactc gtccgctcct
     2461 cgacgtgctt ttcgacgtcc ggccgtttga cccatagcgt ccgcagcttt cgctccgcca
     2521 cgaaatgcag ctggtcggaa ttgtgcttcg atccgggctg gctggattcg ccataactca
     2581 tcacgcccat cgccttgatt ggggtggaga actccaccat cgagacccag gtctcgcccg
     2641 cattcacttt gcgctggccg ttctccagct tgtcccaggt gatggtgcgg aagatgccca
     2701 gattgccgaa gccgccattg ccggggatgt tcacgtcccc gatatggaag cgcgagactt
     2761 cgccgtacgg acggtcgatg gcgccatact gcttcacggt ttcggccgcc gccgtcttca
     2821 ggctggccac gcccaccgac gcatcggcga tgccgcgcgg ggtttccatg gggtcgttgg
     2881 cgtcccattt cacggcatag tttttctggt tcgacatgga gggcccggcg aacgccttca
     2941 cccattcctc gaacaacagc gcgcctttgg aatcggcatt gtattgatga tcccaggctt
     3001 tcagcaccgc gaccgccttc tgcacctcgg cgtcattcga acccgccgcc gctttcaaga
     3061 tttccggcac catgcggtcg gccagaagcg cggtggtggt gtgcttcagg cggatgaaat
     3121 cctcatagga aatcttgccg ctgtcggcca tcagatgcat cgattgttcg ttgcgcatcg
     3181 aaagcggacc ttcgatggcg atataggacg gataatcctt gtaatggatc gcctgcggcc
     3241 aggtgctggt ccacggcgga tcgttggtgt tctgcacgaa accggaggac ggattggcga
     3301 cgtgcggaat ttcctcgaac gtcatcacgt cgctgtcctg ccacagcgtt tcggatgtat
     3361 cgcccggcac caggcctttc caataggcga agtcgccctt cttgcgcttg ggcagaatgc
     3421 cg
//
LOCUS       DASYEG010000220         3442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4422367, whole genome shotgun sequence.
ACCESSION   DASYEG010000220 DASYEG010000000
VERSION     DASYEG010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4422367"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VGM72_09745"
     CDS             <1..811
                     /locus_tag="VGM72_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265590.1"
                     /translation="GMGTRQSAGRGWLVLVSALFCTLAYNLTFFVQELMLTLPKAFVP
                     GVHVWLYHNNHHYEGTDPILPLLQGTGAVADLVVGLIFAALLSGSAGRSMTARLFLFW
                     MAFQGLYAAFSQIVIGALIPQNDMGMAYGYLGLSPTARMAMLAIGLIASVLAGFWLAR
                     KAILLLATTGETLNAGPRMGFLFFAVLLPTLIAGALIIPFRMPRDMIEVTIFPTIVML
                     FGAIWVLIGGALSSTAPRPAHPAPSLAVPLAALIVVLLVFQIVLRPGIAFS"
     gene            1044..1376
                     /locus_tag="VGM72_09750"
     CDS             1044..1376
                     /locus_tag="VGM72_09750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HEY4265591.1"
                     /translation="MLRIIATAAFFALTLNAAQAAEAPPSINVAFGDLNLSRSADTKI
                     LAERLEIAAAKVCRGAGAGLSPVAEMYVVRGCVKDAIHIALASIASRQTNATRADLIG
                     SRKTLASN"
     gene            complement(1397..1789)
                     /locus_tag="VGM72_09755"
     CDS             complement(1397..1789)
                     /locus_tag="VGM72_09755"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY4265592.1"
                     /translation="MTDQTIRAALERHWAASDAGDFLVEHDIYRDDAVLDYPQSGERI
                     RGREHIRESRYLQPSKKRFTVRRILGGGDLWITEFTLSYDGVPSHAVSIMEFQDGLVV
                     HETQYFADGFAPAPSRAHLVEAIPARIA"
     gene            complement(1817..2269)
                     /locus_tag="VGM72_09760"
     CDS             complement(1817..2269)
                     /locus_tag="VGM72_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265593.1"
                     /translation="MTEETLPSLAEMRALNGAQFSDADYIHFVIGRCSLGPDFIQALV
                     RLLNPELAQIEGRFLVVAVGAPGRYQAYRSQGQSPPEAQYWANLTEISGLFANVGPEL
                     AQEIALLVKDQWQKALDKCLQDQSQETCVLKDEAEGEVFITVRTVNES"
     gene            complement(2461..>3442)
                     /locus_tag="VGM72_09765"
     CDS             complement(2461..>3442)
                     /locus_tag="VGM72_09765"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY4265594.1"
                     /translation="GILPKRKKGDFAYWKGLVPGDTSETLWQDSDVMTFEEIPHVANP
                     SSGFVQNTNDPPWTSTWPQAIHYKDYPSYIAIEGPLSMRNEQSMHLMADSGKISYEDF
                     IRLKHTTTALLADRMVPEILKAAAGSNDAEVQKAVAVLKAWDHQYNADSKGALLFEEW
                     VKAFAGPSMSNQKNYAVKWDANDPMETPRGIADASVGVASLKTAAAETVKQYGAIDRP
                     YGEVSRFHIGDVNIPGNGGFGNLGIFRTITWDKLENGQRKVNAGETWVSMVEFSTPIK
                     AMGVMSYGESSQPGSKHNSDQLHFVAERKLRTLWVKRPDVEKHVEERTSY"
BASE COUNT          622 a         1078 c          986 g          756 t
ORIGIN      
        1 cggcatgggc acacggcaat cggcggggcg cggctggctg gtgctggtgt cggcgctgtt
       61 ttgcaccctc gcctacaatc tcaccttctt cgtgcaggag ctgatgctga ccctgcccaa
      121 ggccttcgtg ccgggggtgc acgtctggct ctatcacaac aatcatcatt atgagggcac
      181 cgatcccatc ctgccgctgc tgcaaggcac gggcgctgtg gccgatctgg tggtgggatt
      241 gatctttgcc gcgctgctgt cgggctccgc gggccgctcg atgaccgcgc ggctgttttt
      301 gttctggatg gcgtttcagg gtctttatgc cgccttcagc cagatcgtga ttggggcgct
      361 gatcccgcaa aacgatatgg gcatggcgta tggctatctg ggcctctccc ccaccgcccg
      421 gatggcaatg ctggcgatcg gcctgatcgc ctcggtgctg gcgggcttct ggctggcgcg
      481 caaagccatc ctgctgctgg ccacgacagg cgagacgttg aatgcgggcc cgcgcatggg
      541 ctttctgttt ttcgccgtgc tgttgcccac gctgatcgcg ggcgcgctca tcattccctt
      601 ccgcatgccg cgcgacatga tcgaagtgac gatctttccc accatcgtga tgctgttcgg
      661 cgcgatctgg gttctgatcg gcggcgcgct gagttcgacc gcaccgcgcc ccgcccatcc
      721 cgcaccgtcc ttggcggtgc cgctggcggc gttgatcgtg gtgctgctgg ttttccaaat
      781 cgtgcttcgg cccggtatcg cgttctcgtg accgcacgct gaccgttttt cgcgcagcgc
      841 tttcaaaagc gcgcggcaac acgcactttc ttgtgctcat cccgtgcgca atcactgcca
      901 tattgcagtg caatatattt tgttgacggc caatcaaaac cgcgcaaaat caatatcgca
      961 gtgcaacgga cgactcgggg aagttgtccg gactgcgagc aggggtgaaa cgctttaacc
     1021 gctatcgtta aaggagtttc gccatgcttc gcatcatcgc caccgccgct tttttcgcgc
     1081 tcactctcaa cgccgcgcag gccgcagagg ctccgccgtc catcaatgtc gccttcggcg
     1141 atctcaacct gtcgcgctcc gccgacacca agattttagc cgagcgtctg gagatcgctg
     1201 ccgccaaagt ctgccgcggc gcgggcgcag gcctgtcacc cgtcgccgaa atgtatgtcg
     1261 tgcgcggatg cgtcaaagac gcgatccata tcgcgcttgc cagcatcgcg tcgcgccaga
     1321 ccaacgccac acgcgccgat ctgatcggta gccgcaagac actggcatcg aactgaccgc
     1381 accatcccgg cgcgcgtcac gcgatgcgtg ccgggattgc ttcgaccaga tgtgcgcgtg
     1441 acggcgcggg cgcaaagccg tcggcaaaat actgcgtctc atgcacgacc agcccgtcct
     1501 ggaattccat gatgctcacg gcatgggacg gcacgccgtc atagctgagg gtgaattcgg
     1561 tgatccagag atcgccgcca ccgagaatgc gacggaccgt gaagcgcttt ttgctgggct
     1621 ggagataccg gctctcgcga atatgctctc gtcctcggat gcgctcgccc gactgcggat
     1681 agtccagcac ggcgtcgtcg cgatagatgt catgctccac cagaaaatcg cccgcatccg
     1741 acgccgccca atgccgctcc agcgccgcgc gtatggtttg gtcggtcatc gcaccctcta
     1801 cgaagtgagc gtccgcctag ctttcgttga ccgttcgcac ggtaatgaaa acctcgcctt
     1861 cagcttcatc ttttaaaaca caggtttcct gagattgatc ttgcaggcac ttgtccaagg
     1921 ctttctgcca ttgatctttt actagaagag cgatttcttg agcgagttca ggtccaacgt
     1981 tcgcgaacaa tccgcttatt tccgtgaggt tggcccaata ttgcgcttcg ggaggcgatt
     2041 gcccctgtga ccgataggcc tgatatctgc caggcgcacc gacagccacg acaagaaatc
     2101 ttccttcaat ctgcgccagc tctggattga gcaaacgaac cagagcttgg atgaaatcgg
     2161 gtcccaggga acaccggcca attacgaaat gtatgtaatc agcatcagaa aattgcgcgc
     2221 cgttcagagc tcgcatttcg gctaacgaag gtaatgtctc ttctgtcaaa acttgccgct
     2281 cccatagcgc gcaggatttt gcggcgtgag caggaggatc gcgcggagcg ggcggagccc
     2341 gtgagcacgt ccgacgaact cacgacgcac cgccctcgcc cgctctgcgg gctcgaagcg
     2401 gggccagctt ttctaagctc gccaatgctc gctaagaaaa gctaggccaa agacaagcgc
     2461 ctaataacta gtccgttcct cgacgtgctt ttcgacgtca ggccgtttga cccacaacgt
     2521 tcgcagcttc cgctccgcca caaaatgcag ctggtcggaa ttgtgcttcg atccgggctg
     2581 gctgctttcg ccataactca tcacgcccat cgccttgatt ggggtggaga actccaccat
     2641 cgagacccag gtctcgcccg cattcacttt gcgctggcca ttttccagct tgtcccaggt
     2701 gatggtgcgg aagatgccca gattgccgaa gccgccattg ccggggatgt tcacgtcccc
     2761 gatatggaag cgcgagactt cgccgtacgg acggtcgatg gcgccatatt gcttcaccgt
     2821 ttccgccgcc gccgtcttca ggctggccac gcccaccgac gcatcggcga tgccgcgcgg
     2881 ggtttccatg ggatcgttgg cgtcccattt cacggcatag tttttctggt tcgacatgga
     2941 gggcccggcg aacgccttca cccattcctc gaacaacagc gcccctttgg aatcggcatt
     3001 gtattgatga tcccaggcct tcagcaccgc gaccgccttc tgcacctcgg cgtcgttcga
     3061 acccgccgcc gctttcagga tttccggcac catgcggtcg gccagaagcg cggtggtggt
     3121 gtgcttcagg cggatgaaat cctcatagga aatcttgccg ctatcggcca tcagatgcat
     3181 cgattgttcg ttgcgcatcg aaagcgggcc ttcgatggcg atataggacg gataatcctt
     3241 gtaatgaatc gcctgcggcc aggtgctggt ccacggcgga tcattggtgt tctgcacaaa
     3301 gccggaggac ggattggcga cgtgcggaat ttcctcgaac gtcatcacgt cgctgtcctg
     3361 ccacagcgtt tcggatgtat cgcccggcac caggcctttc caataggcga agtcgccctt
     3421 cttgcgcttg ggcagaatgc cg
//
LOCUS       DASYEG010000221         2556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3118256, whole genome shotgun sequence.
ACCESSION   DASYEG010000221 DASYEG010000000
VERSION     DASYEG010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3118256"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1850
                     /locus_tag="VGM72_09770"
     CDS             165..1850
                     /locus_tag="VGM72_09770"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEY4265595.1"
                     /translation="MKEPGLNPYALLKRFARDTRGVASVVGALVAILGLSLSALTLDV
                     GHLYLAKRQLQGAVDAAALAAAGDPVNANAIAARVLAANGYTQPATVIAGAYTADTSI
                     PIANRLDTGSSAQRNAVRVTQTIAVTSILAPFLGANGSSNIAATATASQIPVASFAIG
                     TGLATLQNGQLNALLGGLLGTTLSLSLVNYQGLASTNVDALTFLNQLAAQVGVTAGTY
                     GDLANANVTMAQLATAIRTALNIHPNGNDSAALDALNLISLQTPAAASALVSKIVDTA
                     LWQSRHIGSIVQQVPGQITLNVFDLVSAMARAYGPGHIANLSAGVGTIITTKLAVGSP
                     MASVALARVGTTASTAQARLALGISLTTPPLLGTAVSVNLPIFVELASGSATVTAIPC
                     RADGTMVTLTTASQAASARFGSIASDTDFANFGTNPVVQPASAAVKLTILTIPIAVGV
                     SGTFPLAAGAPTAQNFTKSDIDNGIVHSASGNGGLLSGLAGQIHFDSPGGLGGVITAL
                     LNPILDLLRPLLVGIIAPLDGTVDTLLRTLGLRLGTIDTVVHGARCGTPTLVT"
     gene            1859..2320
                     /locus_tag="VGM72_09775"
     CDS             1859..2320
                     /locus_tag="VGM72_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4265596.1"
                     /translation="MPVPPLAPARPPGAHALRTKPESLRVFICDDDQAFAEELASALT
                     ASGFEARTLRDGRTPVEIFELFAPDVVLLDIFMPPPDGFEMMNLIAQHRQRRATSLVL
                     MSGADSGLLDTATHFSMGRGIKPAAVLRKPVRLADILAVCIAHRRAPRAAV"
BASE COUNT          458 a          833 c          730 g          535 t
ORIGIN      
        1 gcctgctcga atgggtcgga agttaggcaa acgctaagag tttgcgtgtc aggttgtgtc
       61 gagacttgtg cgccgatttc gcaacacaag cgcaagcaag acgacacggc gcacacagtc
      121 tttacgcata atctgccggg ctagatctga cgtgtaaggc gctcatgaaa gagccgggcc
      181 tgaatccgta tgcattgttg aagcgctttg cgcgcgatac gcgcggcgtc gcttccgttg
      241 tcggcgcgct ggtggcgatt ttggggcttt cgctcagcgc gcttacgctt gatgtcggcc
      301 atctctatct cgccaaacgc caattgcagg gcgcggtcga cgctgcggcg ctggccgcgg
      361 caggcgatcc ggtaaacgcc aacgccatcg cggcccgcgt gcttgcggcg aatggttata
      421 cccagcccgc aacagttatc gccggtgctt acacggcgga taccagcatt cccatcgcca
      481 accgcctcga tacgggatcg agcgcccagc gcaacgctgt gcgcgtcact cagaccatcg
      541 cagtcacgag tattttggcc cccttcctgg gcgccaacgg ctcgtcgaac atcgctgcca
      601 cagccacggc atcgcagatc cctgtggcct ctttcgcaat cggaaccggc ctcgccacgc
      661 ttcagaacgg ccaattgaat gcgctgctgg gaggcttgct ggggactacg ctttcacttt
      721 ctttggtcaa ctatcagggc cttgccagca cgaatgtgga cgccctcacc tttttgaacc
      781 agctcgcggc tcaagtgggc gtcaccgcgg ggacttacgg cgatcttgcg aacgccaatg
      841 tcaccatggc gcaacttgcc accgccattc gcacggcgtt gaacattcac cccaatggaa
      901 atgacagcgc cgcattggac gcgctcaatc ttatttccct gcagacgccc gcagcggctt
      961 cggcccttgt cagcaaaatc gtcgatacgg cactctggca gtcacgccat atcggcagca
     1021 tcgttcagca ggtgccgggc caaatcacgt tgaatgtgtt cgatctggtt tccgcgatgg
     1081 cgcgcgccta cggcccagga catatcgcca atctgagtgc cggggtcgga acgatcatca
     1141 ccaccaaact tgccgtcggc tcccccatgg caagcgtagc gctggcgcgg gtaggaacca
     1201 cggcttccac tgcccaggcc cggctcgctc tgggcatttc actgacaacg ccgcctctgt
     1261 tgggaacggc tgtctcggtc aatcttccga tttttgtcga gctcgcttcc ggcagcgcaa
     1321 ccgtgacagc cattccctgc cgggccgacg gcaccatggt gacattgacg acggcgtcac
     1381 aagcagcatc cgcccgcttt gggagcatcg cgagcgatac cgatttcgca aatttcggca
     1441 caaaccctgt cgtgcaaccc gcatccgccg ccgtgaagct cacgattctg accattccga
     1501 ttgccgtggg cgtcagcggc acattcccac tcgccgccgg cgcacccacc gcgcagaatt
     1561 tcaccaaaag cgatatcgat aacgggattg tccatagcgc atccggcaat ggcggtcttc
     1621 tttccggtct ggccggccag attcatttcg actcgccagg gggattgggc ggcgtcataa
     1681 ccgccctgct caatcccatt ctcgatctgc tgcgccccct tctggtgggc atcatcgcgc
     1741 ctctggatgg cacggtcgat acgctgctgc gcaccctcgg ccttcggctg ggcaccatcg
     1801 ataccgtcgt tcatggcgcc cgttgcggca ccccaactct tgtgacttag gagaagacat
     1861 gcccgttccc cctctcgcgc ccgcgcgccc tccaggagcg cacgcgttgc gcaccaagcc
     1921 cgagtccttg cgggttttca tctgcgacga cgatcaggca tttgccgaag aactcgcgtc
     1981 ggcgctgacc gcatccggct tcgaggcacg aacacttcgc gatggccgga caccggtgga
     2041 gatttttgag ctcttcgcgc cggatgtcgt cctgctcgat atcttcatgc cgccaccgga
     2101 cggctttgaa atgatgaacc tcatcgccca acatcgtcag agacgggcga cttctctagt
     2161 cctcatgtct ggtgcggatt ccgggctgtt ggacacggcg acgcattttt cgatggggcg
     2221 cgggatcaaa cccgcagccg ttctgcgcaa acccgtccgc ctggcggata ttttggccgt
     2281 ctgcatagcc caccggcgcg cgcccagagc tgccgtctga cggcgtcccg catgcggggc
     2341 gcgcgggtct ctgttgcagc ttcttccgat caagctttgc aatcctgcgc taggctatgg
     2401 gggcatttgc cgctagagga tctttgaagg gtcgccgcga tctcaggatt tcttaacctc
     2461 gccagtttgg cggccgcatg ctgtaatgcc gctggaattc gggattgccc gtcatgtcgg
     2521 aaagcaagac aaagattacc ggcggctgcc tgtgcg
//
LOCUS       DASYEG010000222        14700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1088292, whole genome shotgun sequence.
ACCESSION   DASYEG010000222 DASYEG010000000
VERSION     DASYEG010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 14700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14700
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088292"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VGM72_09780"
     CDS             complement(<1..309)
                     /locus_tag="VGM72_09780"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEY4265597.1"
                     /translation="MHKISIPQSVIDSVIRSRGREHVYDELDPAKTALLVVDMQNGFM
                     MEGVAHSLCREAVEIVPNINRIAAAIRQNGGAVVWIQNAATAETLESWSIRVEMDGPE
                     R"
     gene            complement(377..697)
                     /locus_tag="VGM72_09785"
     CDS             complement(377..697)
                     /locus_tag="VGM72_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020043927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY4265598.1"
                     /translation="MPKITYIEHNDKEHVVEVPAGWTVMEGAVKNRIPGIDADCGGAC
                     ACATCHVYVDPAWAPKLAPRQDMEEQMLDFAQDVKPNSRLSCQIKVTAALDGLVVRTP
                     KSQH"
     gene            complement(714..1919)
                     /locus_tag="VGM72_09790"
     CDS             complement(714..1919)
                     /locus_tag="VGM72_09790"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4265599.1"
                     /translation="MERIVIIGAGQAGVQAAATLRAEGFEGTLTLIGEEPYPPYQRPP
                     LSKAYLAGDFARERLFLKPESFFAETRCDLRLAIAATRIDRAAKTVVLSNGTVLGYDR
                     LLLTTGARVRKVPVPGADLAGVYYLRGIEDVDALRPQFASGARLAVVGGGYIGLEVAA
                     VAVKSGIEVTVVEAAGRVMMRAASAQLSAFYEKEHRAHGVRFQFESMAEAFEGTGRVE
                     AVKTSQGRVATDFVLAGIGVVPNCELAKDTGLVCDNGIAVDEFAATSDPDIFAAGDCT
                     SHPAFTGGRVRLESVQNAIDQAKHAALAMLGKGTAYREVPWFWSDQYDLKLQIAGLAY
                     PGDVTVQRGDPRQRKFSVFHMREGVIAAVEAVNAAPDYIIGRKLIAARARVAPERLSD
                     AAVPVKSLL"
     gene            2071..2529
                     /gene="tsaE"
                     /locus_tag="VGM72_09795"
     CDS             2071..2529
                     /gene="tsaE"
                     /locus_tag="VGM72_09795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEY4265600.1"
                     /translation="MTLKDEAATARLGARIAAALVPGDMVAFSGGLGAGKTALARAIL
                     RARGVEGHVPSPTFTLVQAYDTPGLTLHHFDLYRVEDEAELRELGLEDAREEGVLLVE
                     WPERGMPRSWMDDALRISLSMAPPPAGETARLARIAGPARWREMFARDVP"
     gene            2526..3656
                     /locus_tag="VGM72_09800"
     CDS             2526..3656
                     /locus_tag="VGM72_09800"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY4265601.1"
                     /translation="MNVLSVREDAMTAFLSDSGWGAAQRRPLPGDASTRHYTRLRLGT
                     RSAMLMDQPQSAEAPLATHGASPQERRALGYNALARLAGADCARFIAAAHYLRAQGLA
                     APEVYAADTTHGFLLLEDLGDDLYSDVLARGGDERALYEAAIEALVRLHSEEAPAALA
                     PTKPLFSYDETAQLAEIDLLTEWFLPAATRRTPDPSDVEEHRALWCAALRSVVRESAV
                     FVHRDYHAQNLLWRGGRRGLARVGVIDFQDAVAGPRAYDLVSLLEDARRDVAPELAEA
                     MLQLYLLKAHAAGAAHDSETFRASAALLAAQRNAKIIGIFARLAKRDGKPRYLAHLPR
                     VWRYMERDLSHPVLAPLQAWYDRHIPREMRGALTPDTAGVLS"
     gene            3653..4390
                     /locus_tag="VGM72_09805"
     CDS             3653..4390
                     /locus_tag="VGM72_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY4265602.1"
                     /translation="MTGPTRAMILAAGLGTRMAPLTQEKPKPLIEVNAKPLIDHVIDR
                     LVRGGVNFIVVNVHYMADQIVAHLEKRRARTPGVEIRISDESGTILDTGGAIAKALPL
                     FEGEPFFTHNSDSLWVEGMGSAMTRMKTRWNPDSMDALMLLAPCATAIGYEGRGDFEM
                     DSWGVLKRRQEMNLAPFVWTGLQIVHPRLFDAAPAGRFSINPLWDKAIENGRLEGLRL
                     DGVWIHVGTPKGLQDAEIFLRDLAREP"
     gene            4390..7347
                     /gene="addB"
                     /locus_tag="VGM72_09810"
     CDS             4390..7347
                     /gene="addB"
                     /locus_tag="VGM72_09810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HEY4265603.1"
                     /translation="MAPGRPRVFTIPSGIAFGRALAAGVIAHTGGDPLKLADALVLVP
                     TRRAARSLRESFAAALGGAALLPRIRPLGDVDDESDLFDPSEDALEDIPPIAPLRRLL
                     LLAILVQRWGRAKATALPFTQALSYAGELARFLDESISQGADLTKLKTLVPDDLAAHW
                     QEVVAFLELIAVEWPKLLHAEGAVEPAHLRDAKLRALADELARRPPAAPVIAAGSTGS
                     IPATAELLKTIALLPAGAVVLPGLDTALDAPSWDELDAAHAQYGLKQLLKHIGVDRAD
                     VAEWSPLPENHAGRVARVRFLSEALRPPPTTDAWHDLIGSDRDIASGLDNLALIEARD
                     PREEALTIACALREVLETKGRTAALVTPDRGLARRVAAELTRWDIAIDDSAGAKLSRT
                     PSGAFLALLADAAATRFAPVPLLALLKHPLASGGTERSLFRRNVRAMELATLRGLRPE
                     PGLEGIAARLAKTNAPFALQNWFERLKHLLQPFAETIAAPEATLAELARAHAAATEAL
                     AASDSEPGANSLWRGQAGEAAAELIDSLSREGRDIALVPARHYAESFRALATARAVRP
                     LYNLHPRLAILGPLEARLLDFDLVILSGLNEGQWPPEAATDPWLSRPMREKLGLEAPE
                     RRTGLAAHDFASLAASHNVLLTRALKENGAPTIASRWLLRIKQLANGLSRESALAARH
                     DLLRWARALDESRREPRVARPAPRPPVAARPRKLSITEIETWLRDPYAIYARHVLHLK
                     LLDPIDSEPGPRERGIAVHGALERFLRSFPDALPPDALSHLLSFGDEAFAQAGATPAI
                     LALWRPRFERAARWFLRYETERRRHIAKSLVEVKGTLDIAAGGNFTLRGRADRIDVFS
                     DASASIIDYKTGRPPSNPQINTLHAPQLPLEAAMLLRGGFAEHRATSVRELIHVRLTG
                     GEPAGEDFIADVKNVDGLAAKAWQRLTDHVQKFGYETMPYLSRAMPERTTDKGDYDHL
                     ARVREWTLEREDE"
     gene            7344..10724
                     /gene="addA"
                     /locus_tag="VGM72_09815"
     CDS             7344..10724
                     /gene="addA"
                     /locus_tag="VGM72_09815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair helicase AddA"
                     /protein_id="HEY4265604.1"
                     /translation="MSANVHSSTDPGFSAWVSANAGAGKTSLLTDRVTRLLFAGAKPS
                     RILCLTYTKAAAAEMATRLFHRLGEWALLPDGALAARLTAIGAGLPSAKDLRRARTLF
                     ALALETPGGLKIQTIHSFCQNILARFPVEAGIPARFTVLDDRSTQELMAEARNTVLER
                     AGKGDRALSKAIALLATRAADGRFAEILNFAVGDSSRIRDLLRDHRGEAGFFKHLRHT
                     MGIAEGEDELQILTTFCAGLAGHRAELLRATPWLLSGSKSDQDLGSQLQAFLDSGMSP
                     SGYEHLSRAFLTAAGTARQRLVTKRTADERPDLFAFVQSLCDRVQAVEERRKSAVTVA
                     LTEALMIVAQAVLSEFDALKRERAGLDYDDLILATLALFKRQNAASWVLYKLDGGLDH
                     ILVDEAQDTSPEQWQIVGRLAEEFFSGSGARENAPPRTLFAVGDEKQSIFSFQGADPM
                     QFGKNRAIFKALAEAAKLPFVDLRPEVSRRSARSVLEFVDAVFASEAARDGLTDSGDN
                     IHHDPYRAETGRVEIWPLVKASANEDRDAWNLAVDALPRSSAQIVLAGRIAARIAGWV
                     RDGVRLPGTDDPIKAGDIMILVRRRNAFTEEVIRQLLDLSVPVAGADRMVLLDQIAIA
                     DLVALGRFALLPGDDLNLASLLKSPLVGLSEDALYALAQARTGTLWEELSARKDENAG
                     FGDAHAFLSAALAQADYLPPFEFYARALGKGLRKRILARLGAEAADAIDEFLSLALSH
                     EASHPPSLESFLDWFVKGASDVKRDMEQVGGAVRVMTVHGAKGLEANVVILPDTAQVP
                     DHERREPILYTEDCAFYGVPKILETQAVSAAKIAAQAREMREYRRLLYVAATRAREFL
                     VVCGYENRINSQAVPWYRHLSEAARRIGREEEMGGETIVALGAPLTRGPVAAAPADRI
                     SIALPDFFGRAATPEPSSRILRPSEAAGLEEPVLLSPLADGGKRFRRGLFVHALLARL
                     PDIAPAKREAAALAYLRRQGVAADDAAALTAETLAILTDSAFAALFRPDSRAEVAVAA
                     ELPELGNVRVSGQIDRLAVTEEAVLIADFKTNRPPPVHVDQTPRLYRAQMALYRAALR
                     KIYPAKRIDCALVWTDGARLMALPPALLDAEIARIAGG"
     gene            10816..11136
                     /gene="trxA"
                     /locus_tag="VGM72_09820"
     CDS             10816..11136
                     /gene="trxA"
                     /locus_tag="VGM72_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020054879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEY4265605.1"
                     /translation="MAPVKVTDQSFQADVLDSKTPVVVDFWAEWCGPCRMIAPALEEL
                     ASEYDGKLTVVKINIDENPQTPTKYGVRGIPTLMIFQGGQVAATKVGALPKTKIKEWI
                     DASI"
     gene            complement(11140..12459)
                     /locus_tag="VGM72_09825"
     CDS             complement(11140..12459)
                     /locus_tag="VGM72_09825"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEY4265606.1"
                     /translation="MSFEGAASDAVLVRLLSLHPKRIDLVLDRVERLLAALGHPEKNL
                     PPIIHVAGTNGKGSVCAYLRAMLEGSGYRVHVYTSPHLVHFHERIRIAGALISEEELA
                     ATLEECEVANAGEPITFFEITTAAAFLAFSRHPADALVLEVGLGGRFDATNVIPTPVA
                     SCITPIGYDHQDFLGDSLTAIATEKAGIIKRDAHAIVGPQDEEAKAVLVRRADQLGAP
                     LYVYGEDYHAFEERGRLVYQDNDGLLDLPLPKLIGRHQIANAAIAIAALRRIGGRWPR
                     ESGIEWGLKNVEWPGRLQRLHKGPLIDAAPKGAEVWLDGGHNPHGGAAVAQSMADFEE
                     RASKPLYLISGMLKTKDAVGYFLPFRGLAKHVTTIAIPGEAASLGAGALYDSVREAGL
                     EASPADDLTDAMMQVSAWSRAREEEGSPRILICGSLYLAGKVLRENS"
     gene            complement(12456..13382)
                     /gene="accD"
                     /locus_tag="VGM72_09830"
     CDS             complement(12456..13382)
                     /gene="accD"
                     /locus_tag="VGM72_09830"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEY4265607.1"
                     /translation="MNWISNFVRPRIKGLMGSKPASQDNLWRKCPGCGEMVFHRDLMG
                     TQNVCPQCGHHMRIGAKERFAALFDDGAHERLPAPEVPQDPLRFRDDKRYGDRLKENR
                     TKTGQADAVSAARGNLDALPVIIAVQSFDFMGGSLGLATGAGLVAAMRTAVAERRPFI
                     LIVSSGGARMQEGILSLMQMPRTTIGVDMLNEAGLPYIVVLTDPTTGGVSASYAMLGD
                     VQIAEPGALIGFSGPRVIEQTIRERLPDGFQRAEYLLEHGMIDMIVPRGELKVTLSRI
                     LRLLMKQSAAPRSTNGRGNGDSPGIVVPSTQA"
     gene            complement(13426..14247)
                     /gene="trpA"
                     /locus_tag="VGM72_09835"
     CDS             complement(13426..14247)
                     /gene="trpA"
                     /locus_tag="VGM72_09835"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEY4265608.1"
                     /translation="MSRIASRFAELRARGRAGFIPFITAGDPDLETSFAILQKLPATG
                     ADIIELGVPFSDPMADGPTIQASSTRALKAGMSVKKTLALVERFRAHDEATPIVLMGY
                     FNPIHAYGAEHFLRDACHAGVDGLIIVDLGPEEDGALRSHAAKAALDIVRLATPTTDD
                     ARLPTVLDGAGGFLYYVSVSGVTGTKTFTPRDVSQAISRIRMHSALPVAVGFGVRTPA
                     QAAAIAGIADAAVVGSAIVDRVAGALAKGGTHADIVEETLSFCRSLADAVHDARL"
     gene            complement(14244..>14700)
                     /locus_tag="VGM72_09840"
     CDS             complement(14244..>14700)
                     /locus_tag="VGM72_09840"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044135.1"
                     /note="catalyzes the formation of L-tryptophan from
                     L-serine and 1-(indol-3-yl)glycerol 3-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEY4265609.1"
                     /translation="FLDDPDVTIHGVEAAGEGVETEHHAATLSKGTPGVLHGARSYLL
                     QDSDGQIKEAHSISAGLDYPGVGPEHSWLKESGRVNYVSVTDAEALDAFQLTCRLEGI
                     IPALESSHAIAHVMRIAPKLPSDKLIVLGLSGRGDKDVFTVANALGEKL"
BASE COUNT         2609 a         4784 c         4704 g         2603 t
ORIGIN      
        1 acgctccggg ccgtccatct cgacacggat ggaccagctc tccagcgttt ccgccgtcgc
       61 cgcgttctgg atccaaacca ccgcgcctcc attctgccgt atcgcggcgg cgatccggtt
      121 gatgttgggc acaatctcga cagcctcgcg gcacagcgag tgcgccacac cctccatcat
      181 gaatccgttc tgcatgtcga cgaccagaag cgcggtcttc gccggatcga gctcgtcata
      241 gacatgctct cgcccgcgcg agcggatgac ggaatcgatg acggattggg gaatcgatat
      301 cttgtgcacg gcgaggctcc ttgcgcggga ggcccgcatc atactcctac atcgccggtc
      361 gggcgacagc ctgccgctaa tgctgactct ttggcgtacg gacgacgagg ccgtcgaggg
      421 cggccgtcac cttgatctga caggaaagcc ggctgttcgg cttcacatcc tgggcgaaat
      481 cgagcatctg ctcttccata tcctggcgcg gcgccaattt cggcgcccat gcgggatcga
      541 catacacatg gcaggtggca caggcacagg cccccccgca atccgcatcg ataccgggaa
      601 tgcgattttt caccgcccct tccatgacgg tccagccggc cggcacctcg acgacgtgtt
      661 ccttgtcatt gtgttcgatg taggtgatct tcggcatcaa tggcgttcca tgttcaaaga
      721 agagatttga ccggcacggc ggcgtcgctc agccgctcgg gggcgacacg cgcgcgcgcg
      781 gcgatcagct ttcgcccgat aatatagtcc ggcgcggcgt tcaccgcttc cacggcggca
      841 atcacgcctt cgcgcatatg gaagaccgag aatttccgtt gccgagggtc gccgcgctgg
      901 accgtcacgt cgcccgggta tgccagaccc gcgatttgca gcttcagatc atactgatcg
      961 gaccagaacc agggcacttc gcgataggct gtccccttcc cgagcatggc aagggccgcg
     1021 tgctttgcct gatcgatggc attctggacc gattcgagac gcacccgccc gcccgtgaaa
     1081 gcggggtggg acgtgcaatc gccggccgcg aaaatgtccg gatcggaggt cgcggcaaat
     1141 tcgtccacgg caatgccatt gtcgcagaca agccccgtgt ccttggccag ctcgcaattg
     1201 ggcacgacgc caatgccggc cagaacaaaa tcggtggcga cacgcccctg gctggtcttg
     1261 accgcctcca cgcgcccggt cccctcgaag gcctcggcca tgctttcgaa ctggaaccgc
     1321 accccatggg cccggtgctc cttctcatag aaggccgaca attgcgcgga ggccgcccgc
     1381 atcatcaccc ggccggcggc ttccaccacc gtcacttcta tgccggactt gaccgccact
     1441 gccgccactt cgagcccgat atacccccca cccacaacag ccaggcgcgc cccggaggca
     1501 aattgcggcc gcagcgcatc cacgtcttcg atgccgcgca ggtaatacac cccggcaaga
     1561 tcggctcccg gcaccggaac cttgcgcacg cgcgcgccgg tcgtcagcag gagacggtca
     1621 taaccgagca cggtcccgtt ggagagaacc accgtcttcg ccgcccggtc gatgcgcgtg
     1681 gcggctatcg cgagccgcaa atcgcatcgg gtttcggcga agaaggattc cggcttcagg
     1741 aacagccgct cgcgcgcgaa atcgcctgcc aggtaggcct tggacaaagg cggccgctga
     1801 tagggcggat agggctcctc gccaatcagc gtcagggtgc cctcgaaacc ttcggccctc
     1861 agcgtcgcgg cggcctgcac gcccgcctga cctgcgccga tgatgacgat ccgctccatg
     1921 gctttatcct ggtatttgcc gggcctcggc ggttagcacg cgcagggtac gctgggcaat
     1981 cgaacagaca tcgaatctct gccccaatgc cgcgttgagc cgcgagggtg cgcaagttac
     2041 aaccctccgg tggcaggatt cgagaccgaa atgacgctga aggacgaagc cgcgacggcg
     2101 cgattgggcg cacgcatcgc cgcagccctc gtccccggcg atatggtggc gttttccggc
     2161 ggactgggtg ccggcaagac cgcgctcgcc cgcgcgatcc tgcgcgcgcg cggcgtggaa
     2221 gggcacgtgc cgagcccgac tttcacgctc gtccaagcct atgacacgcc ggggctgaca
     2281 ttgcatcatt tcgatctgta ccgggtggag gacgaagcgg aattgcgcga acttggcctg
     2341 gaggatgcgc gcgaggaggg cgttcttctg gtcgaatggc cagagcgcgg gatgccccgc
     2401 tcctggatgg acgatgcgct ccggatttca ctaagcatgg cgccgccacc ggcaggcgaa
     2461 accgccaggc tcgcgcgcat cgctggtccg gcgcgttggc gcgagatgtt tgcaagggac
     2521 gtcccatgaa tgtgctgagt gtgcgcgaag atgccatgac ggcttttctt tcggactcgg
     2581 gatggggcgc tgcgcagcgc cggcctttgc cgggtgacgc ctccacacgc cattacactc
     2641 ggctccgcct gggaacacgc agcgccatgt tgatggacca gccgcaaagc gcggaagcgc
     2701 ctttggcgac acacggcgcg agcccgcagg aacgccgcgc gctcggctac aacgcgcttg
     2761 cgcgcctggc cggagccgat tgcgcgcgat tcatcgcggc agcgcattat ctgcgggcgc
     2821 agggactggc cgcgcccgaa gtgtatgccg cggacacgac gcatggtttc ctgctgctcg
     2881 aagacctggg cgacgatctt tattcggatg tccttgcccg tggcggcgac gagcgcgcac
     2941 tttacgaagc cgccatagaa gcgctcgtgc ggcttcacag tgaggaagcg cccgcagccc
     3001 ttgctccgac gaaaccgctt ttctcgtatg acgaaaccgc gcaactggcc gaaatcgatc
     3061 tcttgaccga atggttcctg ccggcggcga cacgccgtac gcccgatcct tcggatgtgg
     3121 aggagcatcg cgcattgtgg tgcgcggccc tgcgcagcgt tgtccgggaa tccgccgtgt
     3181 tcgtgcatcg cgactatcat gcgcagaatc ttctttggcg cggcgggaga cgcggacttg
     3241 cgcgcgtcgg cgtgatcgat ttccaggatg ctgtggcggg tccgcgcgcc tatgatctgg
     3301 tctcgctgct ggaagacgcg cgccgcgatg tggcgccgga actcgccgaa gccatgctgc
     3361 agctctatct cctgaaagcc cacgccgccg gcgccgctca cgattccgaa accttccgcg
     3421 caagtgctgc gctgctggcc gcccagcgca atgccaagat catcggcatc ttcgcgcggc
     3481 tggccaagcg cgacggcaag ccccgctatc tggcgcatct gccgcgcgtc tggcgctata
     3541 tggagcgcga tctttcgcac cccgtcctcg caccgctcca ggcgtggtac gaccgccaca
     3601 ttccacgcga gatgcgcggc gcgctgacac cggacactgc gggagtctta tcgtgacggg
     3661 tcccacgcgc gccatgatcc tcgccgccgg gctcggcacg cgcatggcgc ctctgacgca
     3721 ggaaaagccc aagccgctga tcgaagtgaa cgcaaagccc ctgatcgacc acgtcatcga
     3781 ccggttggtg cgcggcgggg tcaatttcat tgtcgtcaat gtgcattaca tggccgatca
     3841 gatcgtggcc catctcgaaa agcgcagagc gcgcacgcct ggcgtggaaa tccgcatctc
     3901 ggacgaaagc ggcaccatcc tcgataccgg aggcgcgatc gccaaggcgc ttcccctttt
     3961 cgaaggcgag ccgttcttca cgcacaattc ggattcgctc tgggtcgaag gcatgggctc
     4021 cgcgatgacg cgcatgaaga cgcgttggaa tcccgacagc atggacgccc tgatgctgct
     4081 cgccccctgc gcgacagcca tcggctatga gggacgtggc gattttgaga tggattcgtg
     4141 gggcgtcctg aagcggcggc aggagatgaa cctcgccccc ttcgtctgga ccggtttgca
     4201 gatcgtgcat ccgcgcctgt tcgacgccgc gcccgcgggc cgattctcca tcaatccgct
     4261 ctgggacaag gccatcgaaa acggccggct agagggcttg cgtctcgatg gggtgtggat
     4321 ccatgtgggg acaccgaagg gattgcagga cgcggaaatt ttcctgcgcg atctcgcccg
     4381 cgagccctga tggcgccggg acggccgcgc gtcttcacca tcccgtccgg cattgcgttt
     4441 gggcgcgcgc tggctgcggg tgtgatcgcg cacaccggcg gcgatccgct caaactcgcc
     4501 gatgcgctgg tgcttgtgcc gacgcggcgt gcggcgcgca gcttaaggga aagcttcgcg
     4561 gcagcccttg gcggagccgc actgctgccg cgcattcgtc cactcggcga tgttgacgac
     4621 gaaagcgatc tgttcgatcc ttcggaagac gcgcttgagg atatcccccc gatcgcgccg
     4681 cttcgccgcc tgctgctgct cgccatactg gtgcagcgct gggggcgggc gaaggcaacg
     4741 gctctgcctt tcacgcaggc gctatcctac gcgggtgaac ttgcgcgctt tctcgacgag
     4801 tctatatcgc aaggcgcgga cctgacgaag ctcaagacgc tcgtccctga tgatttggcc
     4861 gcgcattggc aggaggtggt ggcgtttctc gaattgatcg cggtggaatg gccgaaactc
     4921 ctgcacgccg aaggcgcggt cgagccggcg caccttcgcg atgcaaaatt gcgcgctctt
     4981 gccgacgaac tcgcgcggcg tccccctgct gcaccggtga tcgcggcggg ctcgaccggg
     5041 tccatccccg caacggcgga gctgctgaag acgattgcgc ttcttccggc aggcgccgtg
     5101 gtcctgccgg gactcgacac cgctctcgat gcgccgagct gggatgaact cgacgcggcg
     5161 catgcgcaat atgggctcaa gcaattgctg aagcatatcg gcgtggatcg cgcggatgtc
     5221 gccgaatggt cgcctctgcc cgagaaccat gcggggagag tcgcgcgcgt gcgcttcctg
     5281 agcgaggccc tgcggccgcc accgacgacg gacgcttggc acgatctcat cgggtccgat
     5341 cgcgatattg cgtccgggct cgacaatctg gcgctcatcg aagcgcggga tccgcgcgag
     5401 gaagcactca cgattgcctg cgctctgcgc gaggtgctgg agacgaaggg ccgcaccgcc
     5461 gctttggtca cgccggaccg cggccttgcc cgccgcgtgg cggccgagct gacgcgctgg
     5521 gacatcgcca tcgatgattc cgcaggcgcg aaattgtcgc gaacgccgag cggcgcgttc
     5581 ctggcgctgt tggcggacgc ggcggcaaca cgcttcgcgc ccgtgccttt gctcgcgctg
     5641 ctgaagcatc cgctcgcctc cggcggcacc gaacgcagtc tgttccgccg caatgtccgc
     5701 gcgatggaac tggcgacgct gcgcggtctg aggcccgaac cgggactcga aggcatcgcc
     5761 gcgcggcttg cgaagacgaa tgcgccgttc gcgctgcaga actggttcga gaggctgaag
     5821 catctgctgc aacccttcgc cgaaaccatc gcggcaccgg aggcgacgct tgccgagctt
     5881 gcgcgcgcac atgcggcagc gaccgaagca ttggcggcaa gcgacagcga acccggtgcg
     5941 aacagcctgt ggcgcggcca agccggggaa gcggccgccg aactcatcga ctcgctatcg
     6001 cgcgagggca gagacatcgc gctcgtgccc gcacgccatt atgcggaaag ttttcgcgct
     6061 ctcgcgacgg cgcgcgcggt gcggccgctt tataatcttc atccgcggct ggccattctg
     6121 ggaccgctcg aagcccgcct gctcgatttc gacctcgtga tcctttccgg attgaacgag
     6181 ggacaatggc cgcccgaagc ggccaccgat ccctggttgt cgcgcccgat gcgcgaaaaa
     6241 ctcgggctcg aagcaccgga acggcgcacc ggtctggcgg cgcatgattt cgcaagcctt
     6301 gccgcttcgc acaatgtcct gttgacgcgc gccttgaagg aaaatggcgc accgacgatt
     6361 gcgtcacgct ggctgcttcg catcaagcaa ttggcgaatg ggctgagccg cgaatccgcg
     6421 ctcgccgcgc gccacgacct gcttcgctgg gcgcgcgcgc tcgatgagag ccggcgcgaa
     6481 ccgcgtgtgg cccggcccgc gccccgtcct ccggttgccg cgcgcccgcg taaactttcg
     6541 atcacggaga tcgaaacatg gctgcgcgat ccctatgcga tctatgcccg gcacgtcctg
     6601 cacttaaagc tgctcgatcc gatcgattcc gagcctggcc cgcgcgaacg cgggatcgcc
     6661 gttcacggtg cgctcgaacg attcctgcgg agctttccgg acgcgcttcc ccccgatgcg
     6721 ctttcgcatc tgctgagctt cggcgacgaa gccttcgcgc aagccggcgc gacaccggcg
     6781 atccttgcgc tatggcggcc gcgtttcgaa cgcgccgcgc gctggttctt gcgatacgag
     6841 accgagcggc gccgccatat cgccaaaagc ctggtggaag tgaaaggcac gctcgatatc
     6901 gcggccggcg gcaatttcac gctgcgcgga cgcgcggacc gtatcgatgt attcagcgac
     6961 gccagcgcat cgatcatcga ctacaagacc ggacgtccgc cctccaaccc gcaaatcaac
     7021 acgctgcacg cccctcaatt gccgctggag gccgccatgc tgctgcgagg cggattcgcc
     7081 gagcatcgtg ccacgtcggt ccgcgagctg atccatgtcc gtctcaccgg aggcgagccg
     7141 gcaggcgagg atttcatcgc cgacgtgaag aacgtcgatg ggctggccgc gaaggcgtgg
     7201 caacgcctga cggaccacgt ccaaaaattt ggctatgaga cgatgcccta tctgtctcgc
     7261 gcgatgccgg aacgcaccac cgacaaaggc gattacgatc atctggcgcg ggtgcgcgaa
     7321 tggacgctcg agagggaaga cgaatgagcg ccaacgtgca ttcgtcgacc gatccgggct
     7381 tttccgcctg ggtgtccgcc aatgcgggag cgggcaagac gtccttgctc accgaccgcg
     7441 tgacgcggct gctgttcgcg ggcgccaagc cgtcgcgcat tctctgcctc acctacacca
     7501 aggcggcagc ggcggagatg gcgacgcggc tgttccatcg gctgggcgaa tgggcgctgc
     7561 ttccagacgg cgcgttggcg gcccggctca ctgcgattgg cgccggtctt ccctcggcaa
     7621 aggatttgcg aagagcgcgc acgctgttcg ccctggcgct cgaaacgccg ggtggattaa
     7681 agatccagac catccattcc ttctgccaga acatcttggc ccgcttcccg gtggaggccg
     7741 gaattcccgc gcgcttcacc gtgctcgacg accgcagcac acaagagctg atggcagaag
     7801 cacgcaacac cgttctggag cgggccggaa aaggcgatcg cgcgctgtcg aaggcgatcg
     7861 cgctgctggc cacgcgcgcc gccgatgggc gtttcgcgga aatcctcaat tttgccgtcg
     7921 gcgacagcag ccggatccgc gacctcctgc gcgatcatcg cggcgaagcg ggtttcttca
     7981 aacatttgcg ccataccatg gggatcgccg aaggcgaaga cgaactgcaa atcctgacta
     8041 ccttctgcgc cggtctggca ggccaccggg ctgaattgct gcgcgcaaca ccctggttgc
     8101 tcagcggctc caagagcgac caggatctcg ggtcccaact ccaggcgttc ctggactcgg
     8161 gcatgagccc gagcggatat gaacatctga gccgcgcctt cctgactgcc gccggcacgg
     8221 cgcgccaacg tctggtgacc aagcgaaccg cggatgagcg acccgacctc ttcgcgtttg
     8281 tccaatcgct gtgcgatcgc gttcaagccg tcgaagaacg gcgaaagagc gccgtcacag
     8341 tcgcgctcac cgaagcgttg atgatcgtcg cgcaagccgt gctctcggaa ttcgacgcct
     8401 tgaagcgcga acgcgctggg ctggattacg acgatctgat tctggcgacg ctggcgttgt
     8461 tcaaacgcca gaatgcggca tcgtgggtgc tctacaagct tgacggcggg ctcgatcaca
     8521 ttctggtcga tgaggcgcag gacaccagcc ccgagcaatg gcaaatcgtc ggcagactgg
     8581 cggaggaatt tttctccgga agcggggcgc gcgaaaacgc gccgccgcgc acgctgttcg
     8641 ccgtgggtga cgagaagcaa tccatcttca gcttccaggg cgcggatcct atgcagttcg
     8701 gcaagaatcg cgcgatcttc aaggcgctcg cggaggcggc aaagcttccc ttcgtggatc
     8761 tgcggcccga agtttcacgg cgcagcgccc gcagcgtgct cgaatttgtc gatgcggtat
     8821 tcgcgtccga ggctgcacgc gacggcctca cggattcggg agataatatt catcacgatc
     8881 cttaccgcgc cgagaccggc cgggtggaga tctggccgct ggtgaaagcg tccgcgaacg
     8941 aggatcgcga cgcctggaat cttgccgtcg atgcgctgcc gcgcagcagc gcgcagatcg
     9001 tgctggcggg ccggatcgcg gcgcgcatcg ccggctgggt ccgcgacggc gtgaggcttc
     9061 cggggacgga cgatccgatc aaagccggag atatcatgat cctggtgcgg cggcgcaatg
     9121 ctttcaccga agaggtgatc cgccaattgt tggatttgag cgtgccggtt gccggcgccg
     9181 accgcatggt gctgctggac cagatcgcga ttgccgatct ggtggcgctg ggacgctttg
     9241 cgctgttgcc cggtgacgac ctcaatctgg cctcactttt gaaatcgccg cttgtgggtc
     9301 tatcggagga cgcgctgtat gcattggcgc aagcccgcac cggcacgctg tgggaggaat
     9361 tgtccgcgcg caaagacgaa aacgccggct tcggggacgc gcatgcgttc ctttcggccg
     9421 cgctggcgca ggccgattat ctgccgcctt tcgaattcta tgcgcgtgcg ctcggcaagg
     9481 ggttgcgcaa gcgtatcctg gcacggctgg gtgccgaagc cgccgatgcc atcgatgaat
     9541 tcctgtcgct cgcgcttagc cacgaggcat cgcatccgcc gtcgctggaa agcttcctcg
     9601 attggttcgt gaagggcgcc tctgacgtca agcgcgatat ggagcaggtg ggaggcgctg
     9661 tacgcgtcat gaccgtgcac ggcgccaagg gattggaagc caatgtcgtg atccttccgg
     9721 atacggcgca ggtgccggac cacgaacgcc gcgaacctat tctttatacg gaagattgcg
     9781 ccttctacgg cgtcccgaag atcctggaga cgcaggccgt gtcggcggcg aagatcgccg
     9841 cccaggcccg cgaaatgcgc gagtaccggc ggctgctcta tgtcgcggcc acgcgcgcgc
     9901 gcgaattcct ggtcgtctgc ggttatgaga acaggatcaa cagccaggcg gtcccctggt
     9961 accgccactt gagcgaagcg gcgcggcgca tcggacgcga agaggaaatg ggcggcgaga
    10021 cgatcgtcgc gctcggcgcg cccctgacac gcgggcccgt tgcggcggcg ccggcggacc
    10081 ggatatccat cgcattaccc gattttttcg ggcgggcggc cacgccggaa ccgtcttcgc
    10141 gcatcctgcg gccttccgaa gccgcggggc tggaggagcc ggtgctgctc tcgccgctcg
    10201 ccgatggcgg caagcgcttc cggcgcggtc tcttcgtcca tgcgctgctc gcgcggctgc
    10261 cagacatcgc gccggcgaaa cgcgaagccg ccgccctcgc ctatcttcgg cggcaaggtg
    10321 tggcggcaga tgacgccgcc gcattgaccg ccgagacgct ggccatcctg accgattccg
    10381 ctttcgcagc cctgttcagg ccggacagcc gcgcggaggt cgccgtcgcg gccgagcttc
    10441 ccgaactggg caacgtgcgc gtcagcggtc agatcgaccg gctcgcggtg acggaggagg
    10501 cggtcctgat cgcggatttc aagaccaacc ggccgccgcc cgttcatgtg gatcagacgc
    10561 cgaggcttta tcgtgcgcag atggcgctct accgcgcggc gctgcggaaa atctatccgg
    10621 ccaagcgaat cgattgcgcc ctcgtctgga cggacggcgc gcgtctgatg gcgcttccgc
    10681 cggccctgct ggatgccgaa atcgcccgga ttgcgggcgg ttaggacacc aatatctgtg
    10741 tgttcgcggc cgccttgacc ccaggggttg gcattcctac attccatttc aactccccat
    10801 tcccacgagg tttccatggc gcccgtcaaa gtcaccgatc aaagcttcca ggccgatgtg
    10861 ctcgattcca agactccggt cgtggtggat ttctgggcgg aatggtgcgg gccatgccgc
    10921 atgatcgcgc ccgcgctcga agagcttgcc agcgaatatg acggcaagct gaccgtggtg
    10981 aaaatcaaca tcgacgagaa tccccagacg ccgacaaaat acggcgtgcg cggcattccg
    11041 accttgatga tcttccaggg cggccaggtc gcggccacca aggtcggcgc actgcccaag
    11101 accaagatca aagaatggat cgacgcctcg atctgaggct caggaatttt cccgcaacac
    11161 tttcccggcc aggtacaacg agccgcagat aaggatacgc gggctgccct cctcctcgcg
    11221 cgcgcgcgac caggccgaga cctgcatcat cgcatcggta agatcatcgg ccggcgaggc
    11281 ttccagtccc gcctcccgca ccgaatcgta aagcgcaccg gcccccagac tcgcggcctc
    11341 gccaggaatg gcgatggtcg tgacgtgttt cgcgaggccg cggaagggca ggaaatatcc
    11401 caccgcgtcc ttggtcttca gcatgccgga gatgagataa agcggtttcg acgcacgttc
    11461 ctcgaaatcc gccatgcttt gtgcgaccgc cgcaccgcca tgcgggttgt gcccgccatc
    11521 gagccagacc tccgcgcctt tcggcgcagc gtcgatgagc ggtcccttgt gcagcctttg
    11581 caagcgcccc ggccactcga cgtttttcaa cccccattcg ataccgctct cgcgcggcca
    11641 gcgcccaccg atgcgtctga gcgccgcgat ggcgatggcc gcattggcga tctgatgccg
    11701 gccgatgagc ttgggcagcg gtagatcgag caggccgtca ttgtcctgat agaccagccg
    11761 gccgcgctcc tcgaacgcgt gataatcttc gccatagaca taaagcggcg cgccaagctg
    11821 atccgcacgg cgcacgagca cggctttggc ttcctcgtcc tggggcccca cgatggcgtg
    11881 agcgtcccgc ttgatgatgc cggctttctc ggtggcgatc gcggtaaggg aatcgcccag
    11941 gaaatcctga tgatcgtagc cgatcggcgt gatgcaggac gcgaccggcg tggggatcac
    12001 attggtcgca tcaaagcgac cgccaagccc cacttcgagc accagtgcgt cggccggatg
    12061 gcgcgagaac gcaagaaacg cggccgccgt ggtgatctcg aaaaatgtga tgggctcacc
    12121 ggcattcgcc acctcgcatt cctcgagcgt cgcggcgagt tcctcttcgg agataagcgc
    12181 gccggcgatg cggatgcgct cgtggaaatg caccagatgc ggcgaggtgt agacatgcac
    12241 gcgatagccc gaaccctcca gcatcgcgcg caaataggca cagaccgaac cttttccgtt
    12301 ggtgcccgcc acatggataa tgggcggaag attcttttcc ggatggccga gtgcggccag
    12361 cagacgctcc acgcgatcca ggacaagatc gatgcgctta gggtgaagcg acaacagccg
    12421 cacgaggacg gcatcgctcg cggcgccctc gaagctcatg cctgtgttga aggcacgacg
    12481 ataccgggag agtctccgtt tccacggccg tttgtactgc gtggcgccgc ggattgtttc
    12541 atcaagagac gcaggatgcg cgagagcgtg accttgagct cgccgcgcgg cacgatcatg
    12601 tcgatcatgc catgttcaag cagatattcg gcgcgctgga atccatcggg caggcgctcg
    12661 cggatcgtct gctcgatcac gcgcgggccg gaaaacccga tcagcgcgcc gggttcggcg
    12721 atctgcacat cgcccagcat ggcataggaa gccgatacgc cgcccgtggt cggatcggtc
    12781 agcaccacga tgtagggcag gcccgcctcg ttcagcatgt cgacgccgat ggtggtgcgc
    12841 ggcatctgca tcagggaaag gattccttcc tgcatgcgcg ccccgcccga ggagacgatc
    12901 agtatgaacg gccggcgttc ggccaccgcg gtacgcatgg ccgcgaccag gccggcgccg
    12961 gtcgcaaggc cgagcgaccc gcccatgaag tcgaagctct gcacggcaat gatgacggga
    13021 agcgcgtcca ggtttccgcg cgcggcgctc accgcatcgg cctgccccgt cttggtgcgg
    13081 ttttccttca gccggtcgcc atagcgcttg tcgtcacgga agcgcaaagg gtcctgcggt
    13141 acttccggcg ccggcaaacg ctcgtgcgca ccgtcgtcga acagggctgc gaagcgttcc
    13201 ttggcgccga tgcgcatgtg gtggccgcat tgggggcaga cgttctgcgt gcccatcagg
    13261 tcgcgatgaa acaccatctc gccgcaaccg ggacatttgc gccaaagatt gtcctggctc
    13321 gcgggtttgg agcccatcaa ccccttgatc cgtggacgga cgaaattgga aatccagttc
    13381 ataacaaatg ttctagcacg tttgcgttac gagggtcttt ttgttttata aacgggcatc
    13441 gtgaacggca tcggccaagc tgcggcagaa gcttaacgtc tcttcaacga tatcggcatg
    13501 ggttccgcct ttcgcaaggg ctcccgcgac gcggtctaca atcgcagacc ccacaacggc
    13561 cgcatcggcg attcctgcga ttgcggcggc ctgagccggc gtgcgtacac caaaacctac
    13621 cgcaaccgga agcgcggaat gcatgcggat gcgcgatatg gcctggctta cgtcccttgg
    13681 cgtgaaggtc ttggtgccgg tgacgccaga gaccgagacg tagtagagaa atccgcctgc
    13741 gccatcgagc accgtgggca gccgcgcatc gtcggttgtg ggggttgcga ggcgaacgat
    13801 gtcgagcgcg gctttagcgg catgagaccg caaggcgcca tcttcttccg gccccagatc
    13861 gacgatgatg agtccatcga cgcccgcatg acacgcatcg cgcaggaaat gctccgcgcc
    13921 gtaagcatgg atcgggttaa aatagcccat cagcacgatg ggtgtggctt cgtcatgcgc
    13981 gcggaaacgt tcgaccagcg ccagcgtctt cttcacgctc atgcccgctt tcagcgcgcg
    14041 cgtggaggac gcctggattg taggaccgtc ggccatggga tcggaaaacg gcacgccgag
    14101 ttcgatgatg tcggcacccg tggccggaag tttctgaagg atcgcgaatg acgtctccaa
    14161 atccggatcg cccgccgtga tgaacggaat gaagcccgca cgcccgcgcg cgcgaagctc
    14221 ggcaaaacgc gacgcgatgc ggctcacagt ttctccccga gcgcgtttgc gactgtgaag
    14281 acgtccttgt cgccgcggcc cgaaaggccg agcacgatca gtttatccga cggcagtttc
    14341 ggggcgatgc gcatcacatg ggcgatggca tgggaagatt cgagcgcggg gatgatgccc
    14401 tccaaacggc aggtcagttg gaacgcatcc agcgcttcgg catcggtcac cgagacataa
    14461 ttgacgcggc ccgactcctt gagccaggaa tgttccggtc cgacgccggg ataatcgagg
    14521 cctgcggaaa tggaatgagc ttccttgatc tgtccgtcgc tatcctgaag cagataggac
    14581 cgcgctccgt gcaacacgcc cggcgtgcct ttcgacagcg tcgcggcgtg atgttcggtt
    14641 tcgacgccct cgccggccgc ttcgacgcca tgaatcgtca catcgggatc gtcgaggaac
//
LOCUS       DASYEG010000223         2538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1815877, whole genome shotgun sequence.
ACCESSION   DASYEG010000223 DASYEG010000000
VERSION     DASYEG010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815877"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..863)
                     /locus_tag="VGM72_09845"
     CDS             complement(222..863)
                     /locus_tag="VGM72_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265610.1"
                     /translation="MGGAISIAAHVAVFTVLLWPHEPPARPKPLPDAIMVNVADKPLP
                     PAPPAPEELKPASVQPAMPRFTVVTTEPSEIRASDNSDIMSESQLAGATGVGEGGGGG
                     GGCDIGRLVQQALRRDPLVRTAVVNAGRVGKAILIWDGDWVRSGAQEGKGLSAVREAI
                     MWEVGFAPQACRSQRMHGLVLLSLADGGTRFVVGTNEWRWSDLLGLRGVRSVR"
     gene            complement(1074..1976)
                     /locus_tag="VGM72_09850"
     CDS             complement(1074..1976)
                     /locus_tag="VGM72_09850"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HEY4265611.1"
                     /translation="MRGLCARTFAIIAIMAPAVAHADPVPEKVADVIRAAANDGNPAE
                     LAATVKFAKKANPRSAKEIDQLAAQLQAAADARHKAELMQRGFFDGWSGQGELGASSS
                     SGNTRSTTLALGLNFARHGLFWDHVFNASADYQRDNGIESKGRYFASYSGHYNYSDRM
                     YALGLLSWEADRFSGFDSRMSEAVGLGYFFLKGPSMTLSLEAGPALRQTDYLLGRSEN
                     EIAGRTSVNYLWNILPNLVLTEVMSYYGQSHDSTLTSDTGLTVNLIGALSARASYHVQ
                     YETNPPLGLDQADTLTRLTLVYSF"
     gene            2161..>2538
                     /locus_tag="VGM72_09855"
     CDS             2161..>2538
                     /locus_tag="VGM72_09855"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4265612.1"
                     /translation="MGNLILRDAVPQDAELIATYVRELAEFERLSHECIVSAADFRRF
                     LFEQPRAHAMMAEWDGEPAGFALYFYNFSTFLGRPGLYLEDLFVRPAFRRNGIARGIF
                     QALARKAVAEDCGRFEWSVLDWNE"
BASE COUNT          491 a          771 c          770 g          506 t
ORIGIN      
        1 agaccgtacg cctgttcatg aaccagcacg gcgttgctct gaaccggatg tcgaccattt
       61 cctatggcaa gaaagacccg gttgccagca acaagacccg taccggccgg gcgcagaatc
      121 gtcgggtcgt gatcgttgtg atgtcgtaat tttgactgca taaagaaaag ggccccggtg
      181 atcggggccc ttttcatttt cactgttcct attcactatt tctagcgtac ggaccgcacg
      241 ccgcgcagac ccagaagatc cgaccagcgc cactcgttgg tgccgaccac gaaccgggtg
      301 ccgccatcgg ccagcgatag caacaccaat ccgtgcatgc gctggcttcg gcaagcctgc
      361 ggcgcaaagc ccacttccca cataatcgcc tcgcgaacgg cggacaggcc cttgccctcc
      421 tgcgcgcccg accgcaccca atctccatcc cagatcagaa tcgctttgcc gacccgtcca
      481 gcgttcacga cggctgtgcg gaccagggga tcgcggcgaa gggcttgctg gacaagccgg
      541 ccgatatcac agccaccgcc gccacccccg ccctcaccca cgccggttgc ccccgccaat
      601 tgcgactcgc tcatgatgtc cgaattgtcg gaggcacgga tttcggacgg ttcggtggtg
      661 acaaccgtaa agcgcggcat cgcaggctgt acggatgccg gtttgagctc ctcgggcgca
      721 ggcggggccg gcggcaaagg cttatccgcg acattgacca ttatggcgtc cggtagcggt
      781 ttgggccgcg caggaggctc gtgaggccat aggagcaccg tgaacacggc cacatgcgcc
      841 gcaatgctga tcgcaccgcc catcaggcgc ctgcgccgct gcgcccgcga caggccagga
      901 tcggcggaat gcggtagacg ccctgcaggc gcagggccgt ccgaacgcct tcccgggggc
      961 ggtgtgtttt ttcttctcgt cacaattcca gaagggagca tgcccccgcg gcagaaacgt
     1021 ggcgagactg cgaccgcgcc caaccggaaa gtgtccggcc cggccttatt ggttcagaaa
     1081 gaataaacca gcgtgaggcg cgttaaggtg tcggcctgat ccagacccag cggcgggttg
     1141 gtttcatact ggacgtgata ggaggcccgc gccgacaacg cgccgatcag attgaccgtc
     1201 aatcccgtat cggatgtcag cgtgctgtca tggctttgcc cgtaatagga catgacttcc
     1261 gtgagaacga gattgggcag gatgttccag agatagttca ccgaggtgcg gccggcgatt
     1321 tcgttctccg agcggcccag aagataatcc gtctgacgca gggcggggcc tgcttccagg
     1381 gacaatgtca tgcttggtcc cttgagaaag aaatatccca gaccgacggc ttcgctcatg
     1441 cggctgtcaa agcccgagaa gcgatccgct tcccagctga gcaggcccaa cgcatacatc
     1501 cggtcgctat aattgtaatg gccggaatag ctcgcgaaat aacggccttt gctttcgatg
     1561 ccattgtcgc gctgataatc cgcgcttgcg ttgaacacat ggtcccagaa aaggccgtgc
     1621 cgggcaaaat tcagaccgag cgcgagcgtg gtgctgcgcg tattgcccga agagctggat
     1681 gcgcccaatt cgccctgccc gctccagcca tcgaagaagc cgcgctgcat cagttcggcc
     1741 ttatggcgcg catcggcggc ggcctgcaat tgggcggcca gctgatcgat ttccttggcc
     1801 gaacgcgggt tggctttctt ggcgaatttc accgtggcgg ccagctcggc gggattgccg
     1861 tcattggcgg ccgcgcggat cacgtccgcg actttctccg gcacgggatc ggcatgcgcg
     1921 actgcggggg ccatgatcgc aatgatggca aatgtccggg cgcatagtcc gcgcattcaa
     1981 tcctcccgat tttggatttt tgtacttccc ggattctgcc catgattgca aacacaactt
     2041 gccgggatag agtgcgctga ccgcgacaac atgcgatccg gctgacatgt gtgacggatc
     2101 ggcacgcgat tggggcataa tcggggcaga ttttctgtca aattggaaag gaatatcgcg
     2161 atgggaaatc taattttgcg cgatgcggtt ccgcaggacg cggaactcat cgccacgtat
     2221 gtgcgggagc tcgcggaatt cgaacggctt tctcatgaat gcatcgtcag cgccgcggat
     2281 tttcgccggt ttctttttga acaaccacgc gcccacgcca tgatggcgga atgggacggc
     2341 gaacccgcgg gcttcgcgct ctatttctac aatttttcga ccttcctggg acggcccggg
     2401 ctttatctcg aagatctgtt tgtgcgcccc gcctttcgcc gcaacggtat tgcccgtggg
     2461 atattccagg cgctcgcgcg caaggctgtg gctgaggatt gcgggcgctt cgaatggtcg
     2521 gtgctggact ggaacgag
//
LOCUS       DASYEG010000224        23115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3556046, whole genome shotgun sequence.
ACCESSION   DASYEG010000224 DASYEG010000000
VERSION     DASYEG010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 23115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23115
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3556046"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1719
                     /locus_tag="VGM72_09860"
     CDS             <1..1719
                     /locus_tag="VGM72_09860"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY4265613.1"
                     /translation="GPVASALVAKGVLPEREAISLALRARDSGQTLFYAMALDASFAS
                     DENLYAALAERIGTRFVGSRRELLEAVADTDWLDAKSAEQRGVLLLQSPDDKDFETLE
                     YAALDPCDVLVRDWLKARTGKNVKPVTVLPSAFFETLTYLKARIEAPKRGDESLIPID
                     VSWQQENLLLDQPLSADVPLVVDYILQKSQEQGASDIHLEATEDGMLVRVRVDGILQE
                     LTRMPLELHSAVVSRIKVMAGMDVAERRRPQDGRISIMIRKSLVDVRVSTLPTVLGEK
                     VVMRLLDDEALRPSPEQLGMRDQNLRIILDKITAPHGLIMISGPTGSGKTTTLYTCLS
                     AADRAHRNVITIEDPVEYRLKGVHQMQVNEKIGLTFASGLRTVLRQDPDVIMVGECRD
                     QETSRMAVQAALTGHVVFSTIHANDCISVLTRLLDMKIDSFLVASALTLSMSQRLVRV
                     SCKHCSVMVEGREIQRLLRAEGVSPEKMARLGLDIDDKMPCMHPAGCPQCRHTGYSGR
                     QAVFEMLEINAEMRKLIVSDNFEVDALRMLARESGMVPMIGHGLQLVEEGRTTYSELI
                     RVFGDG"
     gene            1716..2762
                     /locus_tag="VGM72_09865"
     CDS             1716..2762
                     /locus_tag="VGM72_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY4265614.1"
                     /translation="MIGAGVNSDLLVFTRQFTAMVRSNLQLVPALESLARETPRRKLR
                     LALNDVLVKVRAGCDFDRALADHPKIFNSTYVGVVRAGMQSGQLGEALHQVSEYLTSL
                     DRVMTKIRGAMMYPLLLMVSFIGTFHMMVFSILPRFETLFAQYGHALPEPTQFVLAIG
                     HVYAAIWPELLGSTVAATIGFTWWVATPNGRLTYDRLKLKIPIIGPLWRLAALARFAH
                     TLGIQVQNSVTLLESIRVAAPACNNKHVEVSLRTIAADIERGEGIAQAFVKQDLFRGV
                     VQQMISAGEKTGELTEPLKSAATYFESLWIQRLDTVVGLINPALTAIMGLLISGMLIA
                     AFLPVFEVSGLAMP"
     gene            2845..5058
                     /locus_tag="VGM72_09870"
     CDS             2845..5058
                     /locus_tag="VGM72_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY4265615.1"
                     /translation="MPSRTRIAIFHDDSEVAGSIAAFGMQADLESVYPASATDLELSL
                     HDPLMAAVVLDIVGSKSGGFELIERLANAPSHPQVIVVTALDAKTVDSIRRLGNAKGL
                     KLRVFKKDGDAEELRGFLAKLEKREARFGIEHLDDAIARQFLHVEYQPKVPLKSGVEE
                     FSVEALCRLKHPQFGNIYPDQFIAMAEKHGLIARLTDSVACQAFRDLNMWRQSGFLVH
                     LALNVSPELLKTPDWCDQFMRRCAEFQIEPERVTLEITESQSGATLDVALDVLTRLRL
                     KGFTLSIDDFGTGFSSLATLYKLPFSELKIDKSFTFDLQQSEEARALIETTIGMAQRL
                     GLKVVAEGVETEEVFRELRLMGCQHAQGYFISKSIAADKVPQFFVDWNSLMKSEPIHD
                     ENALPKIAIIQSLLSDILNEDASETDATLVLSDFSGLRSPAEEDSTLELTRKIPTLVL
                     QGKVAVALARCQAALRRLEDTPERAPLTAKILQLRHLLEQELIAKDDLDLSIPGGRMR
                     LLPRSTALIGRPSSSKPVDVPVGCRWFSRGEKNLRVFAEGTGWFVEDLGSTNGSWVGD
                     RTLLPGEPYALPFGETLIEIGKHVGDAAPIAISLRRPAANPGAVVVSLRADAPRLAGK
                     AAESKWPSWREDVHAQWILFNERIGLGTASDGAIVLGEAGAEKAAEIWFDEGFWIAPC
                     ADAPLTIADASFLQTAPLPASTDLTIGDARLHTENLGSHAESASEQASPRRAKSG"
     gene            5071..6816
                     /locus_tag="VGM72_09875"
     CDS             5071..6816
                     /locus_tag="VGM72_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265616.1"
                     /translation="MTFLDALFAKTGFRRQGLDTANEAVRDAVLFVSTGDAGWSAELA
                     DAAGSRWKQQMVCAPESDATSPPEQLAIAMARLSAEQPQANVTRIVLLIDDPDLQLVD
                     HRFAKLTNFEPRALKEFGAQQAGGRPVVFGSLPFGASSAREIEKRVLGFLPEEKFGSY
                     FFSLGKLATALAAVIPAGVHALRSESHAGGIFATLRMHGHFSTLLIANGDTGIVAVRQ
                     FAFGTLSLAKAYAAEYGLSLEESSASLKIRSRLPPALAMKDDGVAPEHMTATFEAVFP
                     YLRQLRDDIAATLDYFRYQRLAGRPAHLTLTFTAPVVAGLDTWLAEALDLQVDMAPNA
                     LPTFEDVENPTLNLLDGSRPGLLKLGNQPYEFSHGRLVPITGGPFDMPAPKSGSASKL
                     SIPWLEKILAHTGERFELRRDRIMQPVLAVALAVLFVIANLYFLTMPAQQRLTDSASI
                     YDTAAGSTLAAGKTAREADLAGDVSGALWADSLLSVGKALAPGMKLKRIELVPSAGKT
                     GAAPDLNFAVTGTLPPAGADLKLVAGFIDRLSRDASFSRRFAQLRFTGAAESQDEARH
                     EMLFHVVGLSGSARK"
     gene            6813..7595
                     /locus_tag="VGM72_09880"
     CDS             6813..7595
                     /locus_tag="VGM72_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265617.1"
                     /translation="MSTAFKPALLVCLLLLAGAAQYFIDAVYVSPREKAAHDLAALAG
                     VLQVENAKKPIHRAQENRGTTIPDLLSRVQELAGESGVTLMGAEPLPGDGEQFKLHLT
                     ANYGSFLEFLAHFETLQVDIAGFDVTSAMDEPGALEISLSFNHTSAPNAIRPERIKAF
                     MARLKDAELRDPFNPANSALHLVNERNADDLTWTFRLTSISEIGKVKYATIDGKDYSL
                     GDRIQGFVIGAIGEDHVTLIGMKDGKELQRLLTFRSRSKDRT"
     gene            7606..8424
                     /locus_tag="VGM72_09885"
     CDS             7606..8424
                     /locus_tag="VGM72_09885"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEY4265618.1"
                     /translation="MNLPIEEPIRLARNASSAAKAAFAVYGLSDRGLVRANNEDCWFA
                     DGGLGLALAADGVGGHANGARASQGAVRCIADYIRRARKIIGDDGLTPPEMRERIVAR
                     AIALANRRLVIANTAVPDARHQCGTTIVGLWAPHGVHSPATLFHVGDSRLYRLRDGRI
                     DAVTRDHSAYQKWCDTGRHGTAPPKSYILQALGLSAVVPDIVSIQVCPGDCFLLCTDG
                     LTNNVADAELEGELAANSALETVCERLVSLGLARGGSDNLTAVCCAFSRLEATP"
     gene            8421..10361
                     /locus_tag="VGM72_09890"
     CDS             8421..10361
                     /locus_tag="VGM72_09890"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEY4265619.1"
                     /translation="MMRSKRAAVIFLIPLCLFFGARAALAQTSSIPWNQSLTVSRTSS
                     DEDIKTVLRSLLQANGTSVIFTPEVQGPISFRLNKVPIAAAFDQLIQEHNLAYGYNPA
                     TNTVNVTTLAADSQRVKSGSFVPLDAVSYDELLRAMMNFGLSAQNLKYDAGTRTVALS
                     GDAEHVQQIADLIKTLETAHQRQKERQGADRTRELQLKRAELAQQAFEELKHFQVKVI
                     PLRFTDVGPTTKQFQGRTVTIPGIADTLTSILGIAPPSPGAQPPPATAPGMLPPDSSL
                     TDMPGESDNTLLGEAKQLGHPRLSIDQRTNSVVVQGTAEAIASVEKLVHDLDRPLQMV
                     QIEVTIAQADLGVARQLGIDWRSSALSPGGTPFSGAIDTGTAPPQIGNNSTGTGFSTT
                     GLDALSLLPIAGTAGQTTASFVVKGAEGALQAQLQALASKDRARILSAPRLITLDNIT
                     ARITRSQNIFVQVDTRGATGGGLGGVGLQEIQTGLTLEITPSIVPAETDRNEALVRLN
                     LRAENSSPGAGVFGQIDVKSQEVQTNVLVPDGATFVIGGLFDDSQIVNESGVPGLKDI
                     PLIGSLFRNSTNQKTMGETIFFITPHLVDERSVLQDDIAVKVGSEDYIRRERRALTTE
                     VHDPSNGTSSREPNLSHLEEDE"
     gene            10358..10798
                     /locus_tag="VGM72_09895"
     CDS             10358..10798
                     /locus_tag="VGM72_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265620.1"
                     /translation="MKAATYIRGALIVFILGSALILGLAGAFAGSMRERLVFAPHERD
                     TEGAVRQLVEREKALRAAKGNFVAFSNADVEKNASLLGLAWSTFPVKEYFFDAIALES
                     GNIRLRALPRADCVASLSIRARLYIAELSPAGEIVHSGWYPSTD"
     gene            10816..11436
                     /locus_tag="VGM72_09900"
     CDS             10816..11436
                     /locus_tag="VGM72_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265621.1"
                     /translation="MMRIILTAIGMALGAGLALAAPEASGTDIVAAPQWHAGSTWEFS
                     DGYGLRVASVANGTTSFERMDAPGQWFSMRGFLRQDAQSATAKRQTIYRSVPPNAGDT
                     LSESQPLTFQREYLSNKQTIVHATSWSVEGRQTISVPAGTFDCWVIVWRARSLKGDWT
                     GFERWWYSPEAQLYVRMEYKYGAMATMSRVLMRYHLARAEEPAPRP"
     gene            complement(11433..12260)
                     /locus_tag="VGM72_09905"
     CDS             complement(11433..12260)
                     /locus_tag="VGM72_09905"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEY4265622.1"
                     /translation="MSNPAMFLLQSAAGASLGAVAGSFLNVVIHRVPRLIDSHEGAVS
                     VSEYVSGLAWPPSHCPGCSHRLAWRDNVPILSYFMLGGRCRFCRQAYGPRYLVVEIAA
                     AAAFAFCTATLGFSANAFLSALFLGGLIALTAIDIEEQLLPDVLLAPLFCLGLAFHGL
                     YGAGIPDALWGAAAGYGVLRLIGESYRLYSGVDGMGYGDVKFAGVIGAWLGLAAMPVV
                     FAIAFTSGVALMLPFALSGRLASRTAIPFGPFLAFGGFCAFAMPGLAAAMIRLFVPV"
     gene            complement(12257..12607)
                     /locus_tag="VGM72_09910"
     CDS             complement(12257..12607)
                     /locus_tag="VGM72_09910"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY4265623.1"
                     /translation="MPSGTFPGRQNPSDRRRERRYEVALHGELRYDGGSFAVQIADVS
                     GSGAMIFMENPPDAGSEAELWIADYGVLPIEIMHAGDDFCGIAILNPAQHRDRLLSWL
                     RQEIATESPAATVR"
     gene            complement(12724..13353)
                     /locus_tag="VGM72_09915"
     CDS             complement(12724..13353)
                     /locus_tag="VGM72_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265624.1"
                     /translation="MRARDHRQSGNILVPVMLIGLTSTLILGALINASVYLEQTAVED
                     RLAELRAYWATMGHFRYALSRQRHAGLCIKSLGCTVLLDTIKDTDKITALQGYLDEIS
                     SLRTFTYPEENSGYSIKINLTAAVDDDPGRNTFSGHLMITSSYPTAGVSVLPVLSGLA
                     QRFTPYQIRFCTSLTAAYGACGDVSSNNNNGKPTGLYSIKRFYRMQSVS"
     gene            complement(13350..13949)
                     /locus_tag="VGM72_09920"
     CDS             complement(13350..13949)
                     /locus_tag="VGM72_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265625.1"
                     /translation="MRKTSQAGFTLFEVMAASVASIAVIAPAIVFMLDAYDWYAEVQS
                     ELTLNREARQAFDLLGNGAKIASNGKDGFAYVYGIRGRNAAPAGAQRSNYVLQYTNNN
                     VTISGDSFALMTVTCSATGRPLPDCPGAGPTARRSVAGWIGSDVSIENNARSIATGRT
                     VEMMVTVTDPFQAQRAASSAPATETYRTVFTLNRDVPDP"
     gene            complement(13949..14701)
                     /locus_tag="VGM72_09925"
     CDS             complement(13949..14701)
                     /locus_tag="VGM72_09925"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY4265626.1"
                     /translation="MAAISSPAQTACVSAGWKERASAMRSRSNARCAHAGRQRGFSLM
                     EVMFASMILSIFILGVGGFWYSASSRASELVLKQKAIFVLNGEMERISALYVFTGFAA
                     DLLSGPVSTSGYDGIAAIPATRLVYPDGIGTYASNDYVTTSAVTFASSDFYVLLQSNL
                     FSSLNRTYVWIDKNRSVVGRLSWVTTNISVGSCLQLLDCSCQPADNLLLENQCKRLDV
                     FLEYPYRIDGSGNITPPATLLTLSLKTIVGRA"
     gene            complement(14616..15242)
                     /locus_tag="VGM72_09930"
     CDS             complement(14616..15242)
                     /locus_tag="VGM72_09930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY4265627.1"
                     /translation="MRRSQRGFTLIELMVVVTVIGILASIAVPIYSNYVREARLNEAK
                     PYLMDIAARERLYKFKNGVYCCSNGLDETVTGPALGIDLTATGNFCFVIVCRDATLCA
                     SPAATNFITPSEAGDPTAEFEVWAILRATSTTSVAGPQSSTCIMSTSKRTPTGWVSAS
                     GSGLAGSEGRAVVYRYPPPPNGRDIVTGANGVRFSWVEGTSVSHALTQ"
     gene            complement(15412..15915)
                     /locus_tag="VGM72_09935"
     CDS             complement(15412..15915)
                     /locus_tag="VGM72_09935"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY4265628.1"
                     /translation="MRSAYLLSFAALLSLATTADAWAAAAGSPTRTITMISSAGARGL
                     VEGCTAWAQKNNTVVAMAVLDWGGNLIESHAMEGSAPNAIDTALLKAKNALRWRRPSS
                     ETAQIVKSGQNLAPTYIMGDFPQPGAVPIMLNGQVIGSMGVSSGDGEKCAAAGIESVF
                     GKQAAAK"
     gene            complement(15975..17324)
                     /locus_tag="VGM72_09940"
     CDS             complement(15975..17324)
                     /locus_tag="VGM72_09940"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4265629.1"
                     /translation="MTTDASIAASAPAAGWQSGLTAQHWRVLWGSYLGWIFDGYEAFA
                     LIVALPPALHTLLRPDQVPSAAIYAGLAIGITLLGWGIGGLAGGILADYFGRKRMMTL
                     SILFYAGLTGLTAFSTSLAMLIAMRFLTGLAIGAEWSTGIALVAESWPDRARPKGLGF
                     LQSGFGAGSFLAAIVWFVLSLTQPLGDETWRLIFLAGGLPGFCVFYLMRSLGESERWL
                     AAVREKRWSAVEGESHGAGPDAKRPFTLATLFRSAEARRRVWLTFLLSLASTTGWWAV
                     STWAPVYAEQLAKAQGEVAGVWGPRIALIYTLGGLLAYMSSGFVADWLGRRAYLLVTF
                     AGSLVITWMTYLWTGNLYVFALIAFLNGVITLGFGFSWMAIYPVELFTATVRSTAASV
                     VFNGARLIAWVFPILAGTLVAKFGGITNAALIISSIYAIGLFVPWFLPETVGKPLPH"
     gene            complement(17438..19027)
                     /locus_tag="VGM72_09945"
     CDS             complement(17438..19027)
                     /locus_tag="VGM72_09945"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY4265630.1"
                     /translation="MPYRDLRDYIDTLNARGLLHIIDEPICRDTELVPLVRLQFRGLI
                     EEQRKAFWFRQVTDSHGRVYNGSVVLGALGSSRAVYGAALGVTADEIAAAWARANAKH
                     LPPREIAREHAPVKNIILRRPDLGGGADRFPHLISTPGFDPAPFITAGVWVTRDPETG
                     SYNLGIYRGMIKAPDRIGCATDVPTQHLAIQWEKARKLRRPLDAAIFIGGPPALLLAA
                     ASKLAYGVDEYGLAGALNGAPIDVVRCETFDAVVPAHAEIVIEGKIRTDLLEPEAPFG
                     EASGYLGPRKMEKIFEVSAITQRERPIYQGIISEFPPSESTVIRKAAFDAIYLNHLKN
                     ACNIPSVTKVASHEMASCNMMFVIQLDHPAPGQPQAALSAAAAFDSSLGKIFIAVDSD
                     IEPNSMDAVLWALSYSMQPHRDVEIIRGKVPRLDPSVTPGPDERYPDGLGASAMLINA
                     CREHPYLPVSLPRRDFMEAAQTRWKALGLPALDLKSPWHGYPLPAWTPENAEEAELAV
                     TGRYFETGAKLATRRAPPDST"
     gene            complement(19027..20265)
                     /locus_tag="VGM72_09950"
     CDS             complement(19027..20265)
                     /locus_tag="VGM72_09950"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4265631.1"
                     /translation="MHDSSVQPLPLAAATPRVRTRWLVCFLLLLAVTTAFFDRINIAV
                     LFTNRAFQDDIGTSDPALMGLLMTAFVFPYGASAVLLSFVGDVFGPKRTLASIAAILA
                     VTMASMGMITSYPLMLGARAVIGITEGPQFATATVTVKRWFPPREQGLANAFWTIGSP
                     LGSLIGFPAVIFLVAQYGWRASFYLLAGLNAFVVLPIVWLFLKDRPADAPEPATGHQL
                     PFRTALGMLARDWRFWLLPLNNSGTLIYLWGLNSWLPSYLQQARHFNLAMTGFYSALP
                     FVFVIAGQLFFGWLGDRTGRRAAVCGGSLFMTGVFCYLAAIAPDADFAAWCLAISAGF
                     WGGATPTIFALSMQIIPREVAATGFGILAGLANIAGSFAPFIIGVLINTTGNFTAGLE
                     FLVLSCMVCSLAMLPLVRTH"
     gene            20447..20974
                     /locus_tag="VGM72_09955"
     CDS             20447..20974
                     /locus_tag="VGM72_09955"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY4265632.1"
                     /translation="MRIIDAGRLERGRERAAKAARGADRHLYFHGVAEARYVLRKVFR
                     LVEEEAKRAGIDPLAHQALIQICGSQTGALKVKDLAERLDITPAFASSLVKSLAVKGF
                     VVTARDKQDSRVNWIRVTRQGRALLHRIDEQVQVHVDYFTRTLSAQQTEAALAILMFY
                     AGVSLKPRPGDVKKA"
     gene            21026..21532
                     /locus_tag="VGM72_09960"
     CDS             21026..21532
                     /locus_tag="VGM72_09960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY4265633.1"
                     /translation="MTADDATFRNSMRLLASGVTIVASASAGWRSGLTATAVCSLALT
                     PPRVLVSINMQGHTYEVVVQSRCMSVNLLAHDQESLACVFAAKRAAEDEDRFVHGTWK
                     AGATGAPMLFDALAALDCRVTEIIPLDSHAILIGVVEDIVFGPRRSPLIHFEAAYTSI
                     LTSPENSS"
     gene            21529..23007
                     /locus_tag="VGM72_09965"
     CDS             21529..23007
                     /locus_tag="VGM72_09965"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEY4265634.1"
                     /translation="MNAPQTRSLRTGAEYLESIREDGRRIYFEGELVKDVTTHPAFRG
                     AAHSMAGLFDVAADPANAARMTFPSPVTGAPVWRCYQIPKTKEDLAGRRAMSARWAEE
                     TFGLMGRTPDHVAGFLAGYAAKPSVFAEAGAQYAENVVRYHEYARDHHLYLSYAIVPP
                     QIDRSKPAHKQSDPTLHAGVVGERDGGIVLKGAQQLGTGAVFSDAVYISCIHPLGPGD
                     EAYAFAAAVPINAPGLKIYPRRSYAAGVTSRFDYPLSSRFDEVDSLIVLDDVFVPWEN
                     VFCYRNLQLARDQWWRTPSHSYGNHQAQIRYGVKLRFLMGLTKRLCEITGVDTMPPVQ
                     IMLGEMAAYATIVDSMIRAQEFEATIDAEGVVWPSKSALYAVMALQSEINPKLLNIAR
                     ELAGGSIIMLPSAEKDFDSPEMAADLERYIASPGYPSRLRTQVLKLVWDLIGTEFAGR
                     HEQYEKFYGGASFLVKQNMARAYDFAGATRLVDRALALSNTD"
BASE COUNT         4555 a         6996 c         7145 g         4419 t
ORIGIN      
        1 ggtccggtcg cgagcgccct tgtcgcgaag ggggtgctgc cggaacgcga agccatttcg
       61 ctggcgttac gggcgcgcga ttccgggcag accctgttct acgccatggc gctggatgcg
      121 agctttgctt ccgatgaaaa cctttatgcc gccctcgccg agcgtatcgg cacgcgcttc
      181 gtcggttcgc ggcgcgaact tctggaggct gttgccgaca ccgattggct ggatgcgaaa
      241 tccgccgaac agcgcggtgt cctgctgctg caatcgccag acgacaagga tttcgaaacg
      301 ctggaatatg cggcgctcga tccatgcgac gtcttggtcc gcgactggct gaaagcccgc
      361 acgggaaaga acgtcaagcc ggtcaccgtg ctgccgtccg cgttttttga aacattgacc
      421 tatctcaagg ctcgtatcga ggcgcctaaa cgtggcgatg aaagcctcat tccgatcgac
      481 gtatcctggc agcaggaaaa cctgctcctc gaccagcctc tttccgccga tgttccgctc
      541 gtcgtcgact acatcctgca gaaaagccag gaacagggtg cctccgacat ccatctggag
      601 gcgaccgagg atggaatgct ggtgcgcgtg cgcgtcgacg gcatcctgca ggagttgacc
      661 cgcatgccgc tggaactgca ttcggcggtt gtttcacgca tcaaggtgat ggccggtatg
      721 gacgtcgccg aacgacgccg cccgcaggat gggcgcatca gcatcatgat ccgaaagtcg
      781 cttgtcgatg tgcgcgtctc cacattgccg accgtcctcg gcgaaaaagt ggtgatgcgg
      841 ctcctcgacg acgaggcctt gcgtccgagt ccggagcagc tcggcatgcg cgaccagaat
      901 ttgcgcatca ttctcgacaa gatcaccgca ccgcacggcc tcatcatgat cagcggcccc
      961 acgggatcgg gcaagacgac gacgctctac acgtgtcttt cggctgcgga tcgcgcgcat
     1021 cgcaacgtga tcacgattga agatccggtg gaataccgtc tcaaaggcgt gcaccagatg
     1081 caggtgaacg agaagatcgg cctcaccttc gcgagcggcc tgcgcacggt tctgcgccag
     1141 gaccccgatg tgatcatggt cggcgagtgc cgcgatcagg agaccagccg catggccgtt
     1201 caggctgcgc tgaccggcca tgtggtgttc tcgaccatcc atgccaatga ttgcatcagc
     1261 gtgctgaccc ggcttctcga tatgaagatc gactcgttcc tggtcgcctc cgccctgacc
     1321 ctttccatgt cgcaacgtct ggtgcgtgtg tcctgcaagc attgttcggt catggtggaa
     1381 ggacgtgaga ttcagcgtct gctgcgcgcc gaaggcgtct ccccggaaaa gatggcgcgt
     1441 ctggggctcg acattgacga taaaatgccc tgcatgcacc ccgccggctg tccgcagtgc
     1501 cgtcacaccg gctactccgg ccgccaagcc gtattcgaga tgctcgagat caacgcggag
     1561 atgcgcaagt tgatcgtttc cgacaatttc gaagtcgatg ctctgcgcat gctcgcgcga
     1621 gaatccggga tggtgcccat gatcgggcac ggattgcagc tcgtggaaga ggggcgcacg
     1681 acctattccg agttgatacg cgtctttggt gacggatgat cggggcgggc gtcaattccg
     1741 atcttcttgt ctttacgcgg caattcaccg cgatggtgcg gagcaatcta cagttggtac
     1801 cggcgcttga aagcctcgcc cgcgagactc cgcggcgtaa attgcggctg gcgctgaacg
     1861 atgttctggt gaaggtgcgg gcggggtgcg atttcgaccg cgctctcgcc gatcatccga
     1921 agatcttcaa ttccacctat gtcggggtgg tgcgcgcggg catgcaatcg ggccagctcg
     1981 gcgaggctct gcatcaggtc agcgaatatc tcaccagtct cgaccgcgtg atgacgaaaa
     2041 tccgcggcgc catgatgtac ccgttgctgc tcatggtgtc gttcatcggc accttccaca
     2101 tgatggtgtt cagcatcctg ccgcgtttcg agacgctgtt cgcccaatat ggtcacgcgt
     2161 tgccggagcc cacgcagttc gttctcgcca tcgggcatgt ctacgccgcg atctggcccg
     2221 aactcctggg ttcaaccgtg gccgcgacaa ttggcttcac ctggtgggtt gcgacgccga
     2281 atggccggct gacctatgac cggctgaagc tgaaaatccc cattatcggc cccttatggc
     2341 ggctggcggc actggcgcgc tttgcccaca cgctgggaat ccaggtgcag aattccgtca
     2401 cgctgctcga atccatccgt gttgccgcac cggcgtgcaa caacaagcat gtcgaggtga
     2461 gcctgcggac catcgccgcc gatatcgaac ggggtgaggg catcgcgcag gcttttgtca
     2521 aacaggacct gttccggggt gttgttcagc agatgatctc ggcgggcgag aagaccggcg
     2581 agctgaccga accgctgaaa tcggccgcca cctattttga aagcctctgg attcagcgcc
     2641 tggatacggt ggtggggctt atcaatcccg cgctgaccgc gatcatgggc ctcctgattt
     2701 ccggcatgct gatcgccgct ttcctaccgg tcttcgaagt ctctggattg gcgatgcctt
     2761 aaatcgaaaa ggcccgataa atttaaccgt ttcttttccc aaataaggca atccttctcc
     2821 agcatagctg gcttgcgagg aagaatgccg tcccggacgc gaatcgccat ttttcacgac
     2881 gattccgaag tcgcgggcag cattgcggca ttcggcatgc aggccgatct tgaatcggtt
     2941 tatcccgcat ccgccaccga tctggagcta tcgctgcatg atccgctgat ggccgctgtc
     3001 gtcctcgaca ttgtggggtc caagagcggt ggattcgagc tgatcgagcg gcttgccaat
     3061 gcgccgtctc atcctcaggt gatcgtggtt accgcgctcg atgccaaaac ggtcgattcc
     3121 atccggcgcc tgggcaatgc gaagggcttg aagcttcgcg ttttcaaaaa agacggcgac
     3181 gcagaggagc tgcgcggctt tcttgccaag ctcgaaaaac gtgaggcgcg cttcggcata
     3241 gaacatctgg acgatgcgat cgcgcgccag tttctgcatg tcgaatacca gccgaaggtg
     3301 ccgctgaaat ccggtgttga ggaattttcc gtggaggccc tttgccgcct caagcatccg
     3361 caattcggca atatctaccc ggaccagttc atagcgatgg ccgaaaagca cggcctcatc
     3421 gccaggctca ccgactcggt cgcctgccag gcgtttcgcg atcttaatat gtggcgccaa
     3481 agcggttttc tcgtgcacct cgccctcaac gtctctccgg aattgctgaa gacacccgat
     3541 tggtgcgacc agtttatgcg ccgctgtgcg gaattccaga tcgaacccga gcgcgtcacg
     3601 ctggaaatca cggaatctca atccggagcg acactcgatg tcgcgctcga tgtgttgact
     3661 cggctgcgcc tcaagggttt cacgctttcg atcgacgatt tcggcaccgg cttctcatcg
     3721 cttgccacgc tttacaaact gccgttcagc gagctgaaaa tcgacaagag cttcaccttc
     3781 gatctgcaac aaagcgaaga agcgcgcgcg ctgatcgaaa ccaccatcgg catggcccaa
     3841 cgtctcggac tgaaggtcgt cgccgagggt gtcgagaccg aagaggtgtt ccgcgaattg
     3901 cgtctgatgg gctgtcagca tgcgcaaggg tatttcatca gcaaatccat cgccgccgac
     3961 aaggtgccgc agtttttcgt cgattggaat tcgctgatga aaagcgagcc catccacgac
     4021 gagaatgcgc ttccgaagat tgccatcatc caatcgcttc tctccgacat cctgaacgaa
     4081 gacgccagcg aaaccgacgc aacactcgtg ctgagcgatt tttccggcct acgttcgcct
     4141 gccgaagaag attccacgct ggaactgaca cgcaaaatcc ccaccctggt gctgcaaggc
     4201 aaggttgccg ttgcactggc gcgttgccag gctgccctcc gtcgccttga agacactcct
     4261 gaacgtgcgc ctcttacggc caagattctg caattgcgcc atttgctgga acaggaactg
     4321 atcgcgaagg acgatctgga cctttccatt cccggtgggc gcatgcggct tttgccacgc
     4381 agtacggccc tcatcgggcg gccgtcttca tccaagccag tcgatgttcc cgtgggttgc
     4441 cgctggttct cgcgtggcga aaagaatctg cgtgttttcg cggaaggaac cgggtggttt
     4501 gtcgaggatc tgggcagcac caatggaagc tgggtgggcg atcggacact gctgcccggc
     4561 gagccttacg cattgccttt tggcgagacg ctgatcgaaa tcggcaagca cgtaggtgac
     4621 gcggcaccga tcgcgatttc cctgcgccgg ccggcggcaa atccgggcgc ggtcgtggtc
     4681 tcgctgcggg cagacgcacc gcgccttgcc ggcaaagcgg cggaaagcaa atggccatcc
     4741 tggcgcgagg acgtgcacgc ccaatggatt ttgttcaacg agcgcatagg gctcggcacg
     4801 gcatcggatg gggccattgt ccttggcgag gcgggcgctg aaaaagcggc cgagatttgg
     4861 tttgatgagg gtttctggat cgcgccttgc gccgatgcgc ctctcacgat tgccgacgcc
     4921 tcgtttttgc aaacggcgcc gcttccggcg agcaccgatc tcacgatcgg cgatgcacgc
     4981 ctgcacaccg agaatcttgg gtcgcacgcg gagtctgcat ccgagcaggc atcgcctcgt
     5041 cgcgccaaat cgggttgacg agcgcgcgcc atgacgttcc tggacgctct gttcgccaaa
     5101 accggttttc gcaggcaagg actggacacg gccaatgagg ccgtccgtga cgcggtcctt
     5161 ttcgtttcca ccggcgatgc tggctggagc gcagagcttg ccgatgcggc aggctcgcgc
     5221 tggaaacagc aaatggtttg cgcgccggaa agcgatgcca cgtcaccacc ggagcagctt
     5281 gcgattgcga tggcccggct ttccgccgaa cagccgcagg ccaatgtcac gcgcatcgta
     5341 ctcctgatcg atgatcccga tcttcaactc gtcgatcatc gttttgcgaa gctcaccaat
     5401 ttcgaacctc gtgcgctgaa ggagttcggc gcgcagcagg cgggcggaag gccggttgta
     5461 ttcgggtccc ttccttttgg cgcctccagt gcgcgtgaaa tcgaaaagcg ggtgcttggg
     5521 ttcctgccgg aagaaaaatt cgggagctat ttcttcagtt tggggaaatt ggcgaccgct
     5581 ctggccgcgg tcattcctgc gggcgtgcat gcgcttcgct ctgagtcgca tgccggggga
     5641 atctttgcga cgcttcggat gcacggccat ttctccacgc ttctgattgc aaatggcgac
     5701 accggaatcg tcgccgtacg acagtttgcg ttcggaactt tgtcgctggc gaaagcctat
     5761 gcggccgaat atggactttc gctggaagaa tcctcggcgt ccctgaaaat ccggtcgcgc
     5821 ctgccgcccg cgctggcgat gaaggatgac ggcgttgcgc ctgagcacat gacggccact
     5881 ttcgaagcag ttttcccgta tctgcggcaa ttgcgcgacg atatcgccgc cacgctcgat
     5941 tattttcgtt accaacgtct ggccggccgc cctgcgcatc tgacgctcac tttcaccgcg
     6001 cccgtcgttg cggggctcga tacctggctg gcagaggcgc tcgatctgca ggtcgacatg
     6061 gccccgaacg cattgcccac attcgaagac gtggaaaatc ccactctcaa tctcctggat
     6121 ggctcacggc ctggcctgtt gaagctcggg aaccagccct atgagttctc tcacggccgt
     6181 ttggttccaa ttacgggagg accgttcgac atgccggcac ccaagtccgg ttccgcatcg
     6241 aagctgtcca ttccctggct cgaaaagatc ctggcgcaca ctggagaacg tttcgaattg
     6301 cgtcgcgatc ggatcatgca acccgttctg gctgtggcgc tcgcggttct gttcgtcatc
     6361 gcgaatctct atttcctgac gatgcccgct caacagcgtc tgaccgatag cgcctccata
     6421 tatgatacag cggccggcag cactcttgcc gcggggaaga ccgcccgcga agcggatctc
     6481 gccggagacg tcagcggcgc tctgtgggcg gacagtctcc tttccgttgg aaaggccctc
     6541 gcgccgggca tgaagctcaa gcgtatcgaa cttgtcccca gcgccggaaa gacgggtgcc
     6601 gcgccggatc tgaatttcgc tgtgaccggt acgcttccgc ccgccggcgc cgaccttaaa
     6661 ttggtcgccg gttttatcga tcgcctatcg cgggatgctt ctttctcgcg gcgtttcgcg
     6721 caattgcgct tcaccggtgc cgctgaatcc caagacgaag cgcgccatga gatgctgttt
     6781 catgtcgtcg gcttgtccgg gagcgcaagg aaatgagcac ggcgttcaag ccggcattgc
     6841 ttgtctgcct gctgttgctc gcgggtgcgg cgcaatattt catcgatgcg gtctatgtca
     6901 gccctcgcga aaaggctgcg catgatcttg ccgccttggc cggcgtgctc caggtcgaaa
     6961 acgcgaaaaa gcccatccat cgcgcgcagg aaaatcgggg gacaacaatt cccgatctgc
     7021 tctcgcgcgt gcaggaactt gccggcgaaa gcggtgtcac cctgatgggg gccgaaccgc
     7081 ttcccggcga tggcgaacag ttcaaactcc acttaacggc caattacgga agcttcctgg
     7141 aattcctcgc gcatttcgaa acgctgcagg tggatatcgc cggcttcgat gtgacttcgg
     7201 cgatggatga gcccggcgcg ctggagattt cgctcagctt caaccatacg tccgcgccca
     7261 atgcgattcg tccggagcgg atcaaggcgt tcatggcgcg cctgaaggac gccgagttgc
     7321 gcgatccctt caatcccgcc aacagcgccc ttcatctggt gaacgaacgc aatgccgacg
     7381 atctcacctg gacgtttcgc ctgaccagca tttccgaaat cggaaaagtc aaatacgcca
     7441 ccatcgacgg caaggactac agcctcggcg accggattca gggcttcgtg atcggcgcga
     7501 tcggcgagga ccatgtcacg ctgatcggca tgaaagatgg caaggagctt cagcgtttgc
     7561 tgacgttccg cagccggtcc aaggaccgca catgagcgcc gggcgatgaa tttgccgatc
     7621 gaagaaccga tccgtctcgc acgcaacgcg tcttccgcgg cgaaagcggc tttcgcggta
     7681 tacggcctca gcgatcgcgg tcttgtccgc gcgaacaacg aagattgctg gttcgccgat
     7741 ggcggtctgg gtcttgccct cgccgccgat ggcgtcggcg gccatgccaa tggcgcgcgc
     7801 gccagtcagg gcgcggttcg atgcatcgcg gactatattc ggcgtgcgag aaaaatcatc
     7861 ggcgatgatg ggttgacgcc gccggagatg cgcgagcgca ttgtcgcgcg cgcgatagct
     7921 cttgccaatc gcaggctcgt cattgccaat acggcggttc ccgacgcgcg tcaccaatgc
     7981 ggcaccacga ttgtcggcct gtgggcgccg catggcgttc attcgccggc gactttgttc
     8041 catgtggggg acagccgcct gtaccggctg cgcgacggcc ggatcgatgc ggtgacgcgc
     8101 gatcattccg cctatcagaa atggtgcgat acgggccggc acgggacagc gcctcccaag
     8161 agctatattt tgcaggccct ggggctttcg gctgtggtgc ctgacatcgt cagcatccaa
     8221 gtgtgcccgg gcgactgctt tcttctctgt acggatgggt tgaccaacaa tgtcgcggat
     8281 gcggagcttg agggcgagct tgccgcgaat tcggcattgg aaacggtttg cgagcggctg
     8341 gtctcattgg ggctcgcgcg cggcggcagc gacaatctga cggctgtctg ttgtgcattc
     8401 tctcgtttgg aggcgacgcc atgatgcgtt cgaagcgggc cgctgtcata tttctgattc
     8461 cgttgtgtct cttcttcggc gcgcgcgccg cccttgcgca gacatcgtcg attccttgga
     8521 accagtcgct gaccgtcagc cggacatcct cggacgaaga catcaagacc gtgttgcgca
     8581 gcctgctcca ggcaaacggc acatcggtga ttttcacgcc cgaggtgcag ggaccgattt
     8641 ccttccgctt gaacaaggtt ccgatcgcgg cggccttcga ccagctgatt caggaacaca
     8701 atctcgccta tggctataat ccggcgacca acacggtcaa tgtgaccacg cttgcggccg
     8761 attcgcagcg cgtcaaatcg ggaagtttcg tgccgctcga cgcggtgagc tacgatgagc
     8821 tcttgcgcgc catgatgaac ttcggcctct ccgcgcagaa cctcaaatat gatgccggta
     8881 cgcggacggt cgcgctcagc ggggatgccg agcatgtgca acagatcgcc gatctcatca
     8941 agacgcttga aaccgcgcat caacggcaaa aggaaagaca gggtgcggac cgcacgcgcg
     9001 aactgcaact gaagcgcgcc gaactcgctc aacaggcctt tgaggagctc aaacattttc
     9061 aggtcaaggt gattcctctg cgcttcaccg atgtcgggcc cacgacaaag caattccagg
     9121 gccgcaccgt gaccattccg ggtatcgccg atacgctgac gtccattctt ggcattgcac
     9181 caccgtcgcc gggcgcacag ccgccgcccg cgaccgcgcc gggaatgctg ccgccggatt
     9241 cgtccctgac ggatatgccg ggcgagtccg acaatacgct tctcggtgaa gccaaacagc
     9301 ttggccaccc gcgcctttcc atcgatcagc gcaccaattc ggtggttgtc caaggcactg
     9361 ccgaggcgat tgcttccgtg gaaaagctcg tccatgacct cgatcggcct ttgcagatgg
     9421 tgcagatcga agtgacaatc gcgcaggccg atctcggtgt cgcgcggcaa ttggggatcg
     9481 actggcgcag ttccgccctc agtccgggcg gtacgccgtt ctccggtgcg atcgacaccg
     9541 gcactgcgcc gccgcaaatc ggcaacaaca gcacgggaac gggattcagc accacgggtc
     9601 tggacgcgct gagcctgttg ccgatcgcgg gaacggccgg ccagacgacc gcgtctttcg
     9661 tcgtgaaggg cgccgaaggc gcgttgcagg cacagcttca ggcgcttgca tccaaggatc
     9721 gcgcacgcat cctgtccgcg ccccgcctga tcacgctcga taacatcacc gcgcgtatta
     9781 cccgcagcca gaacattttc gtgcaagtcg acacaagagg ggcgacgggc ggaggattgg
     9841 gtggcgtggg cctgcaggaa atccagaccg gcctcacgct cgaaattacg ccctccatcg
     9901 taccggccga gaccgatcgc aacgaagcgc tggtgcggct caatctccgg gcggaaaatt
     9961 cctcgcccgg cgccggcgtc ttcggtcaga tcgatgtcaa gtcgcaggaa gtgcagacca
    10021 atgtgctggt gcccgatggc gccaccttcg tgattggcgg cctgttcgac gacagccaga
    10081 tcgtcaacga atcgggcgtg cccgggctca aggacatccc cttgatcggt tcgctgttcc
    10141 gcaacagcac aaaccagaag acgatgggcg agacgatctt tttcatcacc ccgcatctgg
    10201 tcgatgaacg cagcgttctc caggacgata tcgcggtgaa ggtcggctcc gaagattata
    10261 tccgtcgcga aagacgcgcg ctgacgaccg aggtccacga tccgtcgaac ggcacatctt
    10321 cccgtgagcc gaacctgagc catttggaag aagacgaatg aaagctgcga cctacatccg
    10381 tggcgcgctc atcgtcttca tcctgggatc tgcgctgatc ctgggcctcg ctggcgcttt
    10441 cgccggttcc atgcgcgagc gtctcgtgtt cgctccccat gagcgggaca ccgagggcgc
    10501 ggttcgccaa ttggtggagc gcgagaaagc cttgcgtgcc gccaagggaa attttgtcgc
    10561 tttttccaat gccgatgtcg agaagaacgc gtccttgctg ggccttgcct ggagcacgtt
    10621 tcccgtgaag gaatatttct tcgacgcaat cgcgctcgaa tccggaaaca tccgattgcg
    10681 cgctctgccg cgtgccgatt gcgtggcgtc gctttccatt cgtgcgcggc tttacatcgc
    10741 cgagctttcg cccgcaggcg agattgtcca ttcgggttgg tacccttcaa cggattaggt
    10801 gttgctggca tgcgcatgat gcggattatt ctgacggcaa taggaatggc gctcggcgcc
    10861 ggacttgcgc tcgctgcgcc ggaggcatcc ggtacggaca tcgtcgccgc gccgcaatgg
    10921 cacgccggtt ccacctggga attcagcgac ggctatggtc ttcgtgtcgc gtccgtggca
    10981 aacggcacga catcgttcga acggatggat gcgccgggcc aatggttttc catgcgcggg
    11041 ttcttgcgcc aggatgcgca atcggcgacg gccaaacgcc agacgattta tcgcagcgtg
    11101 ccgccgaatg cgggcgatac cctgagcgaa tcccaacctt tgaccttcca gcgcgaatat
    11161 ctttccaaca agcagaccat cgtccacgcg acctcctgga gcgtcgaagg ccgtcagacc
    11221 atttccgtgc ccgcgggcac tttcgattgc tgggtgattg tctggcgcgc gcgcagtctg
    11281 aagggcgatt ggaccgggtt cgaacgctgg tggtatagcc ccgaggcgca gctttacgtg
    11341 cggatggaat ataaatacgg cgcgatggcg acgatgtcgc gcgttctgat gcgctatcat
    11401 ttggcgcggg cggaagaacc ggcccctcgc ccctagacgg gaacgaacag acggatcatc
    11461 gcggccgcga gacccggcat ggcgaatgcg caaaacccgc cgaaggccag gaacgggccg
    11521 aacggaatgg ccgtacggct cgcgagccgg cccgatagcg cgaaaggaag catgagcgca
    11581 acgccgctgg tgaaggcaat tgcgaatacc accggcatgg ccgccaagcc gagccaggct
    11641 ccgatcacgc ccgcgaattt cacgtcacca taccccatgc cgtccacgcc ggaatacagc
    11701 cggtagcttt cccctatcag ccggagaacg ccatagcccg cggctgcgcc ccaaagcgca
    11761 tcgggtatgc ctgcgccgta gaggccgtgg aacgcgaggc cgagacagaa aagcggcgca
    11821 agaagcacat cgggaagaag ctgctcttcg atatcgatgg cggtgagcgc gatcagaccg
    11881 ccgaggaaga gcgcgctgag aaatgcgttg gcagagaacc ccagcgtcgc cgtgcaaaag
    11941 gcaaaggccg cggcggctgc gatttccacc acgagatagc gcggaccata ggcctggcgg
    12001 cagaagcggc agcgcccgcc cagcatgaaa taggaaagga tgggaacatt gtcccgccat
    12061 gccagacgat ggctacagcc aggacagtgc gacggcggcc aggctagccc ggaaacatat
    12121 tccgagacgg agacggcccc ttcatgagaa tcgataaggc ggggcacgcg atgaatgacg
    12181 acgttgagaa agcttccggc gaccgcgccc agcgaggctc ccgctgcgga ttgcaagagg
    12241 aacatcgcgg gattgctcat cgcacggtcg ctgccggact ttccgttgcg atttcctggc
    12301 gcagccagct gagcaagcga tcgcggtgtt gggcgggatt cagaatcgcg atgccgcaaa
    12361 aatcgtcgcc ggcgtgcatg atctcgatcg gcaggacccc gtagtcggca atccaaagct
    12421 cggcttcgct tccggcgtct ggcggatttt ccatgaaaat catcgcaccg gagccggaaa
    12481 catcggcaat ctgcacggcg aaggaaccgc cgtcatagcg caactcccca tgcagcgcga
    12541 cctcgtagcg ccgctccctg cggcgatcgg aagggttctg tctcccaggg aaagttccgc
    12601 ttggcatcgg catgtcctgt ggcccgcatc ggcaggatgc gtcttgatgc ttaacgccgt
    12661 gctaacggct gcacggccag aggttaatgg ctgacgctgc cgggtttcct ggcgcaagcc
    12721 tggctaagaa acggattgca tccggtagaa gcgttttatg ctgtaaagcc cggtcggctt
    12781 tccattgttg ttattgctgc tgacgtcgcc gcaggcccca taggccgcgg tgaggctggt
    12841 gcagaatcgg atctggtatg gcgtgaagcg ctgtgcaagt cccgaaagca cgggaagcac
    12901 gctgacgccg gccgtcggat aggaactcgt gatcatcagg tgcccgctaa aggtattgcg
    12961 cccgggatcg tcgtcaaccg cggcggtcaa attgatcttg atggaatagc cgctgttttc
    13021 ttcggggtag gtgaacgtgc gcaaggaaga gatttcgtcc aggtagcctt gcagcgcggt
    13081 gatcttgtcg gtgtccttga tggtatcgag cagcacggtg caaccgagac tctttatgca
    13141 aagtcccgcg tgacgttgcc tgctgagcgc ataacggaaa tgccccatgg ttgcccaata
    13201 ggcgcgcagc tccgccagac ggtcttcgac cgccgtctgc tccaaataga cggatgcatt
    13261 gatcagagcc ccaaggatca gggtgctggt gaggccaatc agcatcaccg gaaccaagat
    13321 attgccgctt tggcgatgat cgcgggctct catgggtcgg gaacgtcgcg gttgagcgtg
    13381 aagacagtac gataggtttc cgtggcagga gcggaactag ccgcacgctg cgcctgaaag
    13441 ggatcggtga cggttaccat catctcgacc gtgcggccgg tcgcgatgct gcgtgcgtta
    13501 ttttcgatgg agacgtcgct gcctatccat cccgcaacac ttcgtctggc ggttgggccg
    13561 gcgccgggac agtcaggcaa tggtctgccc gtcgcgctgc atgtcactgt cataagggcg
    13621 aaagaatcgc ccgagattgt gacgttgtta ttcgtatatt gcagaacgta attgcttcgc
    13681 tgcgctcccg ccggcgccgc attgcgtcct cggatgccgt aaacataagc gaagccgtct
    13741 tttccgttgc tggcgatctt cgccccattg ccgagaagat cgaaggcctg ccgcgcctcg
    13801 cgattgagtg tgagttcgct ctggacctcg gcataccaat cataagcatc cagcatgaag
    13861 acgattgcgg gtgcgatgac cgcgatgctg gccaccgagg cggccatcac ctcgaacaat
    13921 gtgaacccgg cttgtgaggt cttgcgcatc aggcgcgtcc gacaatggtc ttgagcgaca
    13981 gcgtcagaag cgtcgccggc ggcgtgatgt tcccggaacc atcgatgcga tagggatatt
    14041 cgaggaacac atccagccgc ttgcattggt tttcgagcaa cagattgtcg gccggctggc
    14101 aggagcaatc caggagctgc agacaggacc cgacactgat gttcgttgtg acccaactca
    14161 gccgcccgac gacgctgcgg ttcttgtcga tccaaacata ggtgcgattg agggatgaaa
    14221 agagattcga ctgaagcagg acataaaaat cggagctggc gaaggtgacg gccgacgtgg
    14281 tcacgtaatc gttcgacgca tacgtcccga tgccgtcggg atagaccagt cgcgtcgccg
    14341 gaattgccgc gatgccatcg taaccgctgg tgctgaccgg cccgctcaaa aggtccgccg
    14401 cgaagccggt gaaaacataa agcgcgctga tgcgctccat ttcgccattg agcacgaaga
    14461 tggccttttg cttcagcacg agttcggaag cgcggctgct ggccgaatac cagaacccgc
    14521 cgacacccag aatgaaaatg gaaaggatca tcgaggcgaa catcacctcc atcagcgaga
    14581 atccccgctg acggccggca tgcgcgcatc gagcgttact gcgtgagcgc atggctgacg
    14641 ctcgttcctt ccacccagct gaaacgcacg ccgtttgcgc cggtgacgat atcgcggcca
    14701 ttcggcgggg gcgggtaacg ataaaccacg gcgcggcctt cgctccccgc aaggcccgaa
    14761 ccggacgccg agacccagcc ggtcggtgtg cgtttgctcg tgctcatgat gcaggtggag
    14821 ctttgcggtc ccgcgacgct cgtggttgaa gtcgcgcgca agatcgccca aacctcgaac
    14881 tcggccgtcg ggtcgccggc ttccgagggc gtgatgaagt tcgtggccgc gggcgacgca
    14941 caaagcgtgg catcccggca gacgatgaca aaacagaaat ttccggtagc cgtcaggtcg
    15001 atgccgaggg cgggaccggt gaccgtttcg tcgaggccgt tgctgcagca atagacgcca
    15061 ttcttgaatt tgtagaggcg ttctcgcgcg gcaatgtcca tcagataagg cttggcttcg
    15121 ttcagccggg cctcgcgcac ataattgctg tagatcggca ccgcgatgga agcgaggata
    15181 ccgatgacgg tcacgacgac catcagttcg atgagggtga atccgcgctg gctccggcgc
    15241 atggatgggt ccggcaattc gattttgctt cgaattgtta accccccggt ggttcacgcc
    15301 ccgtaaagag gcctaaacaa cgctcccacc atggttaacg tggcatttca tgcatagaaa
    15361 agccgggcag cgatgtctcg tcgcccggct tgaacaattt cgaaagcgaa attatttcgc
    15421 cgcggcctgc ttgccgaaca cggactcgat gcctgcggcg gcgcattttt cgccgtcgcc
    15481 gctggacacg cccatggagc cgatcacctg tccgttcagc atgatcggga ccgcgccagg
    15541 ttgcggaaaa tcgcccatga tataggtggg tgcgagattt tgtccgctct tgacgatctg
    15601 ggcggtctcc gagcttgggc ggcgccagcg cagcgcgttc ttggccttca agagtgcggt
    15661 gtcgatggcg ttcggtgcgg aaccttccat ggcgtgggat tcgatcaggt tcccgcccca
    15721 atcgagcaca gccattgcga cgacggtgtt gttcttctgg gcccaggcgg tgcagccttc
    15781 cacgaggccg cgggccccgg cggaactgat catggtgatc gtccgcgtcg gcgagccggc
    15841 cgcggcggcc caggcatcgg cagtggtcgc caaactcagc aaagcggcaa acgaaagaag
    15901 ataggcgctg cgcatgtgtt tcctccccat catggtgtgc ccagtctata ggtcccggga
    15961 tcgagcgtcg aggatcagtg gggcaggggc tttcccaccg tttccggcag gaaccaggga
    16021 acgaagagac cgatcgcata gatgctgctg atgatgagcg cggcattggt gatgccgccg
    16081 aacttcgcca ccagcgtacc ggccaggatc ggaaataccc aggcgatcag ccgcgcgcca
    16141 ttgaacacga cactcgccgc ggtcgaacgc acggtcgctg tgaagagttc gaccggatag
    16201 atcgccatcc aggaaaaacc gaagccgagc gtgatgacgc cattgaggaa ggcgatgagc
    16261 gcgaacacat agagattgcc ggtccacaaa taggtcatcc aggtgatgac gagcgatccc
    16321 gcgaaggtga cgagcagata ggcgcgccgt ccgagccaat cggccacgaa gcctgacgac
    16381 atataggcga gaagcccgcc cagcgtgtag atgagggcga tgcgcggtcc ccacacgccc
    16441 gccacttcac cctgcgcctt ggccaattgt tcggcgtaga cgggcgccca ggtggagacg
    16501 gcccaccatc cggtggtgct cgcgagcgac agcagaaagg tgagccacac gcgacggcgt
    16561 gcctccgccg aacggaacag ggtcgcgagt gtgaacgggc gctttgcatc cgggccggcg
    16621 ccatgggatt cgccctccac cgcgctccag cgtttttcac gcacggcagc gagccagcgt
    16681 tcggattccc cgagcgagcg catcagatag aagacacaaa agccgggaag cccgccggcc
    16741 agaaaaatca gacgccaggt ctcgtcgccc aagggctggg tgaggctgag cacgaaccac
    16801 acgatggccg cgaggaacga gcccgcgccg aatcccgatt gcaggaagcc cagccccttg
    16861 ggccgggcgc ggtccggcca ggattcggcc accagcgcga tgccggtgct ccattccgcg
    16921 ccgatggcga gccccgtgag gaaccgcatc gcgatgagca tggcgaggct ggtgctgaag
    16981 gcggtcaagc cggtcagccc ggcgtagaaa aggatggaga gcgtcatcat gcgcttgcgg
    17041 ccgaaataat cggccaatat gccgccggca aggccgccga tgccccaccc cagcagcgtg
    17101 atcccgatag cgagccccgc atagatggcg gcagatggga cttggtcggg acgcagcaat
    17161 gtgtggagcg cgggcggcag cgcgacgatc aaggcgaagg cttcatagcc gtcgaagatc
    17221 cagccgagat agctgcccca cagcacacgc caatgctgcg cggtcaagcc cgattgccag
    17281 cctgccgcgg gcgcagaagc cgcgatggat gcgtcagtgg tcattcgatc ccagaagtga
    17341 aaaacacgaa gaacacgaag catgcacgaa gcgcacaaag cgttcaaacg aatgatctct
    17401 ttgtgaaccc ttcgtgttct tgtgtctgtc tatgcggtca agtgctgtcg ggaggggcgc
    17461 ggcgcgtggc gagtttcgcg ccggtctcga aatagcgccc cgtcaccgcc agctccgcct
    17521 cttcggcgtt ttccggcgtc catgccggca gcggatagcc atgccagggc gatttcaggt
    17581 cgagcgccgg cagacctagc gccttccacc gcgtctgcgc cgcttccatg aaatcgcggc
    17641 gcggtaacga taccggcaga tagggatgtt cgcggcaggc attgatcagc atggccgagg
    17701 cgcccagccc gtccggatag cgttcgtcgg gtccgggcgt gacggaagga tccagcctgg
    17761 gcaccttgcc gcgaatgatc tcgacatcgc gatggggctg catggaatag gaaagcgccc
    17821 acagtacggc gtccatcgaa ttgggctcga tatcggagtc tacggcgatg aagattttcc
    17881 cgagcgagga atcgaacgcc gcagcggcgc tcagcgctgc ttgcggctga ccgggggcag
    17941 ggtggtcgag ctggatcacg aacatcatgt tgcagctcgc catctcgtgg gaagcgacct
    18001 tggtcaccga tgggatattg caggcgtttt tcaaatgatt gaggtagatc gcatcgaacg
    18061 cggccttgcg gatgaccgtg ctttcgctgg gcgggaattc gctgatgatg ccctgataga
    18121 tggggcgttc gcgctgcgtg atcgccgaga cctcgaaaat cttttccatc ttgcgtgggc
    18181 cgagataacc gctcgcctcg ccgaagggcg cttcaggttc cagcaaatcg gtgcggattt
    18241 tgccctcgat gacgatttcg gcgtgcgccg gcacaaccgc atcgaaggtt tcacaacgaa
    18301 ccacatcgat gggcgcgcca ttgagcgcgc cggccaaccc atattcatcg acgccatagg
    18361 cgagcttgct cgccgcggcc agaagcagcg caggcggtcc gccgataaag atggcggcgt
    18421 ccagcggccg cctgagcttg cgcgcttttt cccattggat ggcaagatgc tgcgtcggca
    18481 cgtcggtggc gcagccgatg cggtcagggg cctttatcat gccgcgatag atgccgagat
    18541 tgtacgagcc ggtttcggga tcgcgcgtca cccacacacc cgcggtaatg aagggggcgg
    18601 gatcgaagcc cggcgtcgag atcaaatgcg gaaaacgatc cgcaccgccg cccagatcgg
    18661 gacgccgaag gatgatgttt ttcaccggcg cgtgctcgcg cgcgatttcg cgcggcggca
    18721 ggtgctttgc gtttgcgcgt gcccaggcgg cggcaatctc gtcagccgtg acgcccaacg
    18781 cggcgccata gacggcgcgt gagcttccca aagcccccag caccaccgag ccgttataga
    18841 cgcgcccgtg gctgtccgtc acctggcgaa accagaaggc tttgcgctgt tcctcgatga
    18901 ggccgcgaaa ttgtagccgg accagcggga ccagctccgt gtctctgcat atgggctcgt
    18961 cgatgatgtg cagcagtccg cgcgcgttca gcgtgtcgat gtaatcgcgc aaatcacgat
    19021 atggcatcaa tgtgtccgca cgaggggaag catggcaagc gagcacacca tgcaggacag
    19081 aaccaggaat tccagcccgg cggtgaaatt cccggttgtg ttgatcaaga ccccgataat
    19141 gaagggcgca aacgaaccgg cgatgttggc gagccccgcg agaatgccga atccggtggc
    19201 cgcgacctcg cgcgggatga tctgcatgct gagcgcgaat atggtcggcg tcgccccgcc
    19261 ccagaagccg gcgctgatcg caaggcacca tgccgcgaaa tcggcatcgg gtgcgattgc
    19321 cgcgagatag cagaacacgc ccgtcatgaa gagggagccg ccgcacaccg ccgcgcgccg
    19381 tccagtcctg tcgccaagcc agccaaaaaa aagttggccc gcgatcacga acacgaacgg
    19441 caaggccgag tagaagccgg tcattgcgag gttgaaatgg cgcgcctgct gcagatagga
    19501 gggtagccag ctattcagcc cccagagata gatcagcgtg ccggaattgt tcagcggcag
    19561 cagccagaaa cgccaatcac gggcgagcat tccgagcgcg gtacggaagg ggagttgatg
    19621 cccggtggcg ggctcgggtg catcggccgg ccggtccttg aggaagagcc agacgatggg
    19681 cagaacgacg aaggcgttca atcccgccag cagatagaac gacgcgcgcc agccatattg
    19741 cgcgaccagg aaaatgaccg cgggaaatcc gatcagcgat ccgagcgggc tgccgatcgt
    19801 ccagaatgca ttggccaagc cctgttcgcg cggcgggaac caccgcttca cggtcaccgt
    19861 ggccgtcgcg aattggggcc cctccgtgat cccgatcacc gcgcgggccc ccagcatcag
    19921 cgggtaggag gtgatcatgc ccatgctggc catggtgacg gcaaggatcg ccgcgatgga
    19981 tgccagcgtg cgtttgggcc cgaacacatc gccgacgaaa ctgagcagca ccgccgatgc
    20041 gccgtaggga aagacaaatg ccgtcatcaa gaggcccatc agggcgggat cgctggttcc
    20101 gatatcgtct tgaaaggcgc ggttggtgaa caggaccgca atgttgatgc gatcgaaaaa
    20161 ggccgtcgtc accgcgagca gcaaaagaaa gcagacgagc cagcgtgtgc gcacgcgcgg
    20221 tgtggccgcg gcaagcggta aaggctgaac gctggaatcg tgcacgaagt cctccctggc
    20281 aatcgagcat agataaggcg ccgcagggac gccatccttc gccaagctca ggatgaggac
    20341 tttttcccat cctcatgtga gcgcgtcgaa ccatgagagc tttgccattg tgaaagcgca
    20401 cccgacatag ctagtttggt gagccaaatc ggaaaaagaa gagacgatgc gcattatcga
    20461 tgccggccgt ctggagcggg gccgcgaaag ggcggcgaaa gccgcgcgcg gcgccgaccg
    20521 gcatctctat ttccacgggg tcgccgaggc gcgttacgtg ctgcgcaagg ttttccggct
    20581 tgtcgaggaa gaggccaagc gcgccggcat cgacccgctt gcccaccaag ccctgatcca
    20641 gatttgcggc agtcagaccg gcgccctcaa ggtcaaggat ttggccgaac gcctcgacat
    20701 cacgccggcc tttgcctcca gcctggtgaa atccttggcg gtcaagggct ttgtggtcac
    20761 ggcgcgcgac aaacaggaca gccgtgtcaa ctggatcagg gtcacccgtc agggccgcgc
    20821 tttgctgcac cgcatcgacg agcaggtaca ggtccatgtc gactatttca cgcgaacatt
    20881 gtcggcgcag cagaccgagg ccgcgctcgc catcctcatg ttctatgccg gagtttcgct
    20941 gaaaccgcgt cccggagatg tgaagaaagc ttagtagaca aacctatcta aagaacataa
    21001 tcttgaaaag atattggaga atttcttgac ggcagacgac gcgaccttcc gaaattccat
    21061 gcgcctgctt gcgtccggcg tcaccatcgt ggccagtgca tcggccgggt ggcgcagcgg
    21121 attgaccgct accgccgtct gttcgcttgc gctaaccccc ccgcgcgttc tcgtgtccat
    21181 taatatgcag ggccacacct atgaggtcgt ggtccaaagc cgctgcatgt cggtgaatct
    21241 cctggcccat gaccaggaaa gcctggcctg cgtattcgct gcaaagcgcg ccgccgagga
    21301 tgaggatcgc ttcgttcacg gcacatggaa ggcgggcgcg accggggccc cgatgctctt
    21361 cgatgcgttg gccgcgctcg attgccgcgt gacggaaatc atcccgctcg acagtcatgc
    21421 gattctgatc ggcgttgtcg aagacatcgt cttcggtcca cgccgctcgc cgctgatcca
    21481 tttcgaggcc gcttacacat ccatcctcac atcgcccgag aattcatcat gaacgctccc
    21541 caaacgcgta gcctgcgcac cggcgcggaa tatctcgaat ccatccgcga agacggccgc
    21601 cggatttatt tcgagggcga gctcgtgaag gatgtcacga cgcatccggc cttccgtggt
    21661 gcggcccatt cgatggcggg gctgttcgat gtggccgccg atcccgccaa cgccgcacgg
    21721 atgacgtttc cttcgcccgt gaccggcgcg cccgtctggc gctgctatca aattccgaag
    21781 acaaaagaag atctcgccgg ccgccgcgcc atgagcgcgc gctgggccga ggaaaccttc
    21841 gggttgatgg gccgcacgcc cgaccacgtg gcggggttcc tcgccggcta tgccgccaag
    21901 cccagcgtgt tcgccgaagc cggtgcgcaa tacgccgaaa acgtcgtgcg ctatcacgaa
    21961 tatgcgcgcg accatcatct ctatctgtct tacgccatcg tgccgccgca gatcgaccgc
    22021 tcgaaaccgg cgcacaagca atccgacccc acgctccatg cgggagtcgt gggagaacgc
    22081 gacgggggca tcgtgctgaa aggcgcgcag cagctcggta ctggggcggt attctccgat
    22141 gccgtctaca tcagctgcat ccatccgctc ggtccaggag acgaggctta tgctttcgcg
    22201 gctgcggtgc ccatcaatgc gccgggcctg aagatctatc cccggcgttc ctatgccgca
    22261 ggcgtgacca gccgtttcga ttatccgctg tcctcgcgtt tcgacgaggt cgattcgctg
    22321 atcgtgctcg atgatgtgtt cgtgccgtgg gagaatgtgt tctgctaccg caatctccag
    22381 ttggcccgcg accaatggtg gcgcacgccg tcgcattcct atggcaatca tcaggcgcaa
    22441 atccgttatg gggtgaaatt gcgcttcctg atggggctga ccaagcggct ctgcgaaatc
    22501 accggtgtcg acaccatgcc cccggtgcag atcatgctgg gcgagatggc ggcctatgcc
    22561 accattgtgg actccatgat ccgggctcag gagttcgagg cgacaatcga tgcggaaggc
    22621 gtggtctggc cgtccaaatc cgcgctttac gcggtgatgg cgctgcagtc cgagatcaat
    22681 ccgaagctcc tcaacatcgc gcgcgaactg gcgggcggct ccatcatcat gctgccctcg
    22741 gcggaaaagg atttcgattc ccccgaaatg gcggccgatc tggagcgcta tatcgcctcg
    22801 ccgggatacc cgtctcgcct gcgtacccag gtcctgaaac tggtgtggga tctgatcggc
    22861 accgaattcg ccggccgcca cgagcaatat gaaaaattct acggcggcgc gtcgttcctg
    22921 gtgaagcaga acatggcgcg ggcctatgat ttcgcgggcg ccacgcggct ggtcgaccgg
    22981 gctctcgccc tctccaatac ggattgagcg agaagccgtt gcaaaagcgg gcgcgatgct
    23041 cgacaaaccg caaggtttaa gatgagttca ggggacgtcc tatgagtgtt gagcgcaagc
    23101 ccgccgcttt cggcg
//
LOCUS       DASYEG010000225         2721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2687197, whole genome shotgun sequence.
ACCESSION   DASYEG010000225 DASYEG010000000
VERSION     DASYEG010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2687197"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1460
                     /locus_tag="VGM72_09970"
     CDS             <1..1460
                     /locus_tag="VGM72_09970"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY4265635.1"
                     /translation="MKRIFIGIGAIAVLLLAFMAVRTLMVPAPAEEAAAPPIPVDSNA
                     VAQHLAEAVRFQTISYGDGVKETEKSKALNDMRLWMEQTYPYFHEAAGPEQFGDSLLF
                     TWIGKNPNLPPVLLMAHMDVVPVVPGTEKDWMHAPFSGDIAAGFVWGRGTLDDKGQLV
                     SILEAANRLAMMDYQPERTIMFAFGQDEEVGGGKGNAAIAKALTARATHFAWVLDEGS
                     SIMNEPYPGTRKPVAFISNAEKGFLTLELTAHGQGGHAARPSHDLALPRLSSAILNTV
                     GHPFESDLDAIQREKLAVLAPLVPFGDRFVLANLWLTKSLVVQRMESEPDSAAVLHTT
                     ISPTILNAGIKENVIPPTARALINFRLHPRDTIASVTAHVKQAVADPNVDVTEREETL
                     SEATKVVATDSAAYTYVANAIRATYGVPVAPDIMTGATDSRHYLAIADAVLRFRPYHE
                     EVVDLARVHGTNERLAVGDLGPAVGFYTRLIQDLK"
     gene            1457..2449
                     /locus_tag="VGM72_09975"
     CDS             1457..2449
                     /locus_tag="VGM72_09975"
                     /inference="COORDINATES: protein motif:HMM:NF026253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HEY4265636.1"
                     /translation="MNRRGFGACLLAAASVLFAGCERQSRTAASAQSESTAQTGRMVI
                     APQFDEGGPFAEGLAAVRVGARYGYIGTKGQIVIAPQFDFAGVFSEGLAAAGSGSKTG
                     YIGKDGAFVLTPQLEIADLSAFSEGLAAIRIGDFLNGKWGYIDKKGAIVIKPQFDSAG
                     PFSNGLAVIAIDRKWGYIDKAGRHVIAPQFASAGSFADELAAVRDGDDLTGKMGYIGK
                     DGKFVIPPQFDAAFAFSNGLADVRIGDAYSFIDRQGKTIIDPKVDFADSFSNGLAAVR
                     VGDESTGKYGFIDSHGVMIIKPQFDFADLFSVSDGLAAIRMGGEKDGKFGYIGR"
BASE COUNT          519 a          843 c          837 g          522 t
ORIGIN      
        1 gaatgaagcg catcttcatc ggcatcgggg cgattgccgt tctactcctc gccttcatgg
       61 cggtgcggac cctgatggtt ccggctccgg ccgaggaagc ggccgctccg cccattccgg
      121 tggattcgaa cgccgtcgcg cagcatctgg ctgaggctgt ccggttccag accatctcct
      181 acggcgacgg cgtcaaggag acggagaagt ccaaggcgct caacgatatg cggctatgga
      241 tggaacagac ctatccctat ttccacgagg ccgccggccc ggagcaattc ggcgacagtc
      301 tgctgttcac ctggatcggc aagaacccaa acctgccgcc ggtgctcctg atggcgcata
      361 tggacgtggt gccggtcgtt cccggcacgg agaaggattg gatgcacgcg ccgttctccg
      421 gcgatatcgc ggcgggcttc gtctggggcc gtggcacgct cgacgacaaa ggccagctcg
      481 tttccatcct ggaggcggcg aaccgcttgg ccatgatgga ttaccagccc gaacgcacca
      541 tcatgttcgc ctttggccag gatgaggaag tgggcggcgg caaaggcaat gcggcaatcg
      601 ccaaggcgct gaccgcacgc gccacgcatt tcgcctgggt gctggatgag ggcagctcga
      661 tcatgaacga gccctatccg ggcacccgca aacccgtcgc cttcatctca aatgccgaga
      721 agggttttct caccttggaa ctgaccgccc atggccaagg cggtcacgcg gcacggccca
      781 gccacgatct cgcattgccg cgtctttcca gcgcgatcct caatacggtc ggccatccgt
      841 tcgaatccga tcttgacgcc atccagcgcg agaagctcgc tgtgcttgcc ccgctggtgc
      901 cgttcggaga ccgcttcgtg ctcgccaatc tgtggctgac caaatcgctg gtcgtgcagc
      961 gcatggaaag cgaaccggat tcggcggccg tcctgcacac cacgatttcg ccgaccatcc
     1021 tcaacgcggg catcaaggag aatgtgattc cgccaacggc gcgcgcactg atcaatttcc
     1081 gtctgcatcc gcgcgatacg atcgcttcgg tgacggcgca tgtgaagcag gccgtcgccg
     1141 atcccaacgt cgatgtgacc gagcgcgagg agacgctgtc cgaagccacc aaggtcgtgg
     1201 cgacggacag tgcggcctat acctatgtcg ccaatgcgat ccgcgccacc tatggcgtgc
     1261 ccgtggcgcc ggacatcatg acgggcgcca ccgattcgcg ccactatctc gcaatcgcgg
     1321 acgccgtgtt gcggtttcgt ccgtatcatg aggaagtggt cgatctggcg cgcgtgcatg
     1381 gcaccaatga gcgcctggcg gtcggcgatc tcggcccggc ggtggggttc tatacgcgtc
     1441 tgatccagga tttgaagtga acaggcgcgg tttcggcgca tgcctgttgg cggcggcatc
     1501 ggtgctgttt gccggctgcg aacgccagag ccggaccgct gcgtccgcgc aatccgagag
     1561 cacggcgcaa accgggcgca tggtcatcgc gccgcaattc gacgaagggg ggcctttcgc
     1621 cgaaggtctg gcggcggttc gcgttggcgc tcgctatggc tacatcggca cgaagggcca
     1681 aatcgtcatc gcgccgcaat tcgatttcgc cggggttttt tccgaagggc tggccgcggc
     1741 aggctcgggc agcaagaccg gctacatcgg caaggacggc gcgttcgtgc tgacgccgca
     1801 actcgaaatc gccgatctgt cggctttctc cgaagggctc gccgctattc gtatcggcga
     1861 tttcctgaac ggaaaatggg gctatatcga caaaaaaggc gccatcgtca tcaaaccgca
     1921 attcgattcc gcgggaccgt tctcaaacgg gctggccgtt atcgccatcg atcggaaatg
     1981 gggctacatc gataaagccg gcaggcacgt catcgcgccg caattcgcat cggcagggtc
     2041 tttcgccgat gaattggcgg cggtgcgcga cggcgatgac ctcaccggaa aaatgggtta
     2101 tatcggcaag gacggaaaat tcgtcatccc gccgcaattt gatgccgcgt tcgcgttttc
     2161 caatgggctc gccgacgtgc gcatcggcga cgcctacagc ttcatcgacc gtcagggaaa
     2221 aacgatcatc gacccgaaag tggatttcgc ggactcgttc tccaatgggc tggctgccgt
     2281 gcgcgtcggt gacgaatcca ccggaaaata tggcttcatc gacagccatg gcgtcatgat
     2341 catcaagccg caatttgatt tcgccgattt gttttcggtc tccgacggtc tcgccgccat
     2401 acgcatgggt ggcgagaaag acggcaaatt cggctatatc ggccgctaat cgggccggcg
     2461 ctcgcgccca tatgccgttt cgtgttacat gcatgacatc gcgaagtcat attgcgttca
     2521 tccgccattc aaacggatcg cgcgatggtc ggcgcctgga aaaggaaaac acagatgctg
     2581 aatctcacga agaccatgtt gctgggcggc gcgctgatcg cgctgagcat ccctccggct
     2641 tcggcgcaaa tcctggagcg gctgcagcag tgccaatcct ccggcggcca catcgtcaac
     2701 ggcaaatgcg aagtgccgcc t
//
LOCUS       DASYEG010000226         6830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3558363, whole genome shotgun sequence.
ACCESSION   DASYEG010000226 DASYEG010000000
VERSION     DASYEG010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6830
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3558363"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VGM72_09980"
     CDS             <1..302
                     /locus_tag="VGM72_09980"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEY4265637.1"
                     /translation="RAAYQNPAILADVDRRFFGGETRDVNAAVDAYVDYVAARLDEGV
                     PLSAMTKHVLGLFHGQPGARLFRRHLSENATRKGAGISVLRDALGFIGNPRAEAA"
     gene            complement(325..1491)
                     /locus_tag="VGM72_09985"
     CDS             complement(325..1491)
                     /locus_tag="VGM72_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265638.1"
                     /translation="MRGGLERGRGFWLVTRIPESFFWEDCAIAIRDLGRASPKGVHDS
                     LTAKQATTLDQIRSRLDSFHVLNERLSREYSEKGALARSDWWRREYYAFPYLTGAKFE
                     AIIERFCDIFFNTVDMNAEGQLTPHLDFKNEDSFMAKFTHMLEEINARGGMPTDVIER
                     ARTPIVDYFSKGDPIGIRLFAKFERDDAPILVKYSKRQFMQEMYEFGKIRICPASFYS
                     ASSHNGAIRDLETQRPFAIPTFRERLAGESHFEFQGHKIPLDAGNFEIPVEVQDYFLY
                     SLSNHVYYRLPSDFDADAAIIVRDKNAFIQRLVSSFLAANPHYDVHYGDATYYDPCLD
                     FTKIRIPEMSKHFRYSYQREFRIVFNKREPLRTKLVPQFLQIGSMRDYAELITL"
     gene            complement(1514..1936)
                     /locus_tag="VGM72_09990"
     CDS             complement(1514..1936)
                     /locus_tag="VGM72_09990"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY4265639.1"
                     /translation="MTQTAITGGCLCKAVRYTITAEPIVTRICWCRECQYWGAGSSTV
                     NVCFPSNTVSVGGALSDYTSTAASGSPMRRRFCAKCGTQVFSGADTRPHLTFVRAGTL
                     DDREIAKPVMTIWTASAPSWACINDDIPWVPGQPPPAA"
     gene            complement(1955..3451)
                     /locus_tag="VGM72_09995"
     CDS             complement(1955..3451)
                     /locus_tag="VGM72_09995"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEY4265640.1"
                     /translation="MFGAPKSHLVPNTADFENQPLITANGFREYDARWQFPKEINLVG
                     MQALGLALGTYFHECGIEPKVVVGHDFRSYSLAIKQALTLGLMSAGCAVHDIGLALSP
                     VAYFAQFALDAAGVAMVTASHNENGWTGVKMGAQRPLTFGPDEMTRLKEIALCGEGVP
                     RAGGSYTRVANMRETYLDAVTKGHRLSRPIRVVAACGNGTAGAFAPEALKRIGADVVA
                     MDTELNWDFPKYNPNPEDMHMLHAMGEAVIASGAEIALGFDGDGDRCGVVDDEGHEIF
                     ADKVGVLLARDLSSRIGGAKFVVDVKSTGIYEIDPVLRANGVVTDYWKTGHSYIKRRT
                     SELGAVAGFEKSGHYFFNPPVGRGYDDSIVAAIATLEMLDRAGKKISALVRDLPKTWG
                     SPTMAPFCPDERKYDVVDSVIKIYEAIQAGGETILGQKIVRLVTVNGVRVTLEDGTWG
                     LVRASSNKPSLVVVCESPVSDARMRAMFADIDTRLSKFPEIGEYDQKI"
     gene            complement(3517..4086)
                     /locus_tag="VGM72_10000"
     CDS             complement(3517..4086)
                     /locus_tag="VGM72_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265641.1"
                     /translation="MRLIALFAALAFFAAAPATAQSWKEYTYPQYAFAVSFPAEPVVE
                     TKSYQTADGTGAEARVYSVAQANAVFTMTVVDLSGIQTEEKAVIDHAIRTLSQDGDVK
                     LDIPHRISRVYGRQLSIAGRDGSHASIALFYHQKRLYQIQGLALPGEDATADAIRFQQ
                     SLIFTNNAANRTLFEPVIQAVGRVFFGGG"
     gene            complement(4139..4660)
                     /locus_tag="VGM72_10005"
     CDS             complement(4139..4660)
                     /locus_tag="VGM72_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265642.1"
                     /translation="MRAYCILVTAVLCAGGTLAAEGGWQLFSYPESGFAAQYPNPPQV
                     ETVDYKTAQTPDGIVKQRVYSTNNGGVIYSVAVADFTRTRAEKDKTIEEAVANLSGLG
                     KLTHNESGARIDGNYGREIRVEDATGTSYTDAIFFIDNKLYELKVTYPAVNNDPIGSS
                     GIHFFQQTFQLLH"
     gene            4798..5679
                     /gene="cobA"
                     /locus_tag="VGM72_10010"
     CDS             4798..5679
                     /gene="cobA"
                     /locus_tag="VGM72_10010"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEY4265643.1"
                     /translation="MTNSVLSLGTDTRTALRRLAEALSLPRLEPGWVWLAGAGPGDPG
                     LASLHTLHAIAEADVILTDALIDKTLLVLARPDAQIVDAGKRGGKPSAVQETISRRLI
                     AYARKGARVLRLKGGDPFVFGRGAEEALALARAGVPFRVIPGITAGIGGLAYAGIPVT
                     HRDTNQAVTFITGSGAGGRAPDLDWAAIAKGSPTIVLYMARRHAGEIAGHLVNAGRDG
                     NEPAAIVSNAARDGQTTIVTRLAALGEAAALAETPAIIIIGENVRLAAGLDWLGALSG
                     RLLDPDPLGRERLSDAG"
     gene            5747..6280
                     /locus_tag="VGM72_10015"
     CDS             5747..6280
                     /locus_tag="VGM72_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265644.1"
                     /translation="MARAQFHKNQRVYVRPVGTWALIERLVPQWTKGLEEPLRIFYDV
                     GLGRDFGADELEAESSVAPPDSDEELWRVVRAPNKWQSPAESTTHPYPGTYPVIITGE
                     AEWGGWRVPGAEYALSPDRIEYQARMIASIPRLAAIVKKLSEWAEGTPNGDVPLSLRD
                     ILRESDEATAYLTDSVA"
     gene            6421..>6830
                     /gene="pepN"
                     /locus_tag="VGM72_10020"
     CDS             6421..>6830
                     /gene="pepN"
                     /locus_tag="VGM72_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEY4265645.1"
                     /translation="MKTAEPRAIHLRDYAPPPYRIPEIALDFTLDGNATRVTATMKVE
                     RQAESAEPLVLNGHGLKLISVARDGKMLTPPGYAVDSDGLTLADPPAAFTLQIVTEIA
                     PAANTALEGLYMAGGIYCTQCEPEGFRSITYFLDR"
BASE COUNT         1352 a         2093 c         2009 g         1376 t
ORIGIN      
        1 gccgcgccgc ctatcagaac ccggccattc tcgccgatgt cgaccggcgc tttttcggcg
       61 gcgaaacgcg cgatgtgaac gcggcggtgg acgcttatgt ggattacgtt gcggcgcggc
      121 tggacgaagg cgtgccgctc agcgccatga ccaagcacgt gctcggactc ttccacggcc
      181 agccgggtgc gcgtctgttc cgccgccatt tgagcgagaa cgcaacccgc aaaggcgccg
      241 gaatttcggt gctgcgcgat gctctcggct tcatcggcaa tccccgcgcc gaagcggcgt
      301 gaaaattctg ggtcattttg ttggtcagag agtgatcagc tcggcatagt ccctcatgct
      361 cccaatctgc agaaattggg gaactaattt ggtccgaagc ggttcgcgtt tattaaaaac
      421 aatccgaaat tctctttgat atgaatagcg aaaatgcttc gacatttccg gaattcgtat
      481 ctttgtaaaa tccaagcatg gatcatagta agttgcgtcg ccataatgca catcatagtg
      541 cggattagcg gcgagaaacg aactaacaag tcgttgaatg aaggcattct tgtccctcac
      601 aatgatagcc gcgtcggcat cgaagtccga cggcagccgg taataaacgt gatttgataa
      661 gctgtataga aagtaatcct gcacctcgac tggtatctca aagtttccag cgtccaatgg
      721 aatcttgtgt ccttgaaatt caaaatgact ctcgccggct aacctctcgc gaaaggtcgg
      781 gatagcgaac ggccgctgtg tttccaggtc tctgattgcg ccgttgtgcg aagatgcact
      841 gtaaaatgag gcggggcata tgcgtatttt gccaaattca tacatctcct gcatgaattg
      901 tcgtttagaa tattttacta ggattggggc gtcatcacgc tcaaatttgg caaacagtcg
      961 aattccaatc ggatcaccct tcgaaaaata gtcgacaatc ggtgttcttg ctcgctcaat
     1021 cacatctgta ggcatacccc ctcgtgcatt tatctcctcc aacatatgag taaatttcgc
     1081 cataaaggaa tcctcatttt taaaatccaa atgaggagtt agttggccct ccgcattcat
     1141 gtctacggtg ttaaagaaaa tgtcgcagaa tctttcgatt atcgcttcga acttggctcc
     1201 ggttaagtac ggaaaggcgt agtattcccg acgccaccag tcgctgcgcg ccaaagctcc
     1261 cttttctgag tactcccgcg aaagtcgttc gttcaggacg tgaaacgagt ccaatcgcga
     1321 gcgtatctga tcaagcgttg ttgcttgctt tgctgtcagc gagtcgtgaa cgccttttgg
     1381 agaggcacga cctaaatccc gtattgcgat tgcgcaatcc tcccaaaaaa aactttcggg
     1441 aattctcgtt accaaccaaa acccgcgacc gcgttcgagg cccccacgca cgaaacgcca
     1501 agtacctcca ccatcacgcc gccggcgggg gctggccggg aacccacggg atgtcgtcgt
     1561 tgatgcaggc ccagctcggc gcgctcgcgg tccagatcgt catcaccggt ttggcgattt
     1621 cccgatcgtc gagcgtgccc gcgcgcacga aagtgagatg cggccgcgtg tcggcgccgc
     1681 tgaagacctg ggtgccgcac ttggcacaga agcggcggcg catgggagag ccgctggcag
     1741 ccgtgctggt ataatcggaa agcgcgcctc cgacgctcac ggtattgctc ggaaagcaca
     1801 cattcaccgt cgagctgccg gcgccccaat attggcactc ccggcaccag caaatgcgcg
     1861 tgacgatcgg ttcggccgtg atggtgtaac gcaccgcctt gcacaagcat ccgccggtaa
     1921 tcgctgtttg cgtcatcgcg aaatcttttc cgtttcagat cttctggtca tattcgccga
     1981 tttcgggaaa tttcgacagc cgcgtgtcga tgtccgcgaa catcgcgcgc atccgcgcat
     2041 cggacaccgg gctttcgcat accaccacga gcgagggctt gttcgacgaa gcgcgcacca
     2101 ggccccaggt gccgtcctca agcgtcacgc gcacgccgtt caccgtcacc agccgcacga
     2161 ttttctggcc caatatggtt tcgccgcccg cctgtatggc ttcgtagatc ttgatcacag
     2221 aatcgaccac atcatatttg cgctcatcgg gacagaacgg cgccatggtc ggcgagcccc
     2281 aggtcttggg cagatcgcgc accagcgcgg aaatcttttt gcccgcgcgg tcgagcattt
     2341 ccaaagtggc gatcgccgcg acgatggaat cgtcatagcc gcgacccacc ggcgggttga
     2401 agaaataatg cccgctcttt tcaaaaccgg cgaccgcgcc cagctcgctg gtgcggcgct
     2461 tgatgtagga atggccggtc ttccaataat cggtgacgac gccgttcgcg cgcaagaccg
     2521 gatcgatctc ataaatgccg gtcgacttca catcgacgac gaatttggcg ccgccgatcc
     2581 ggctcgacaa atcgcgcgcg agcagaacgc cgaccttgtc cgcgaaaatc tcgtggcctt
     2641 cgtcgtccac cacgccgcag cgatctccat cgccgtcgaa gccgagcgcg atctccgcac
     2701 cggaggcgat gaccgcctcg cccatcgcgt gcagcatgtg catgtcttcg gggttcggat
     2761 tgtatttggg gaaatcccag ttcagttccg tatccatcgc caccacatcg gcgccgatgc
     2821 gcttgagcgc ctcgggcgcg aaggcgcccg ccgtgccgtt gccgcaggcg gccacgacgc
     2881 ggatcggccg tgacaggcga tggcccttgg tcaccgcgtc gagataggtt tcgcgcatgt
     2941 tcgcgacgcg cgtgtagctg ccgcccgccc gcggcacgcc ttcgccgcaa agcgcgatct
     3001 ccttcagccg ggtcatctcg tcgggcccaa acgtgagcgg ccgctgcgcc cccatcttca
     3061 cgccggtcca gccgttttca ttatggctcg cggtcaccat cgccacgccc gccgcgtcca
     3121 gcgcgaattg cgcgaaatag gcgacgggcg acagcgccag accgatatcg tgcaccgcgc
     3181 agcccgccga catcagcccg agggtcagcg cctgtttgat ggcaagcgaa taggaccgga
     3241 aatcatgtcc caccacgacc ttgggctcga tgccgcactc gtggaaatac gtgccgaggg
     3301 caagccccag cgcctgcatc ccgacaagat tgatctcctt ggggaactgc cagcgcgcgt
     3361 catattcgcg gaaaccattg gcggtgatca gcggctgatt ttcgaagtcg gccgtattgg
     3421 gaacgagatg gcttttcggc gcgccgaaca tgatttcgct cttggctcga atgcggacgc
     3481 tgatctagag ccgcaggccg acgaacgcca gaaaattcag ccgccgccga agaagactct
     3541 gccaacagcc tgaatcacag gttcaaacag ggtccggttc gccgcgttat tggtaaaaat
     3601 taaagactgc tggaaacgga tcgcatcggc tgtggcgtcc tcgccgggaa gcgccagacc
     3661 ctgaatctga tagagccgct tctgatgata gaacagggca atcgatgcat ggctgccatc
     3721 gcgtccggca atgctcaatt gccggccata gacccggctg atgcggtggg gaatatcgag
     3781 cttgacgtcg ccgtcctggg acagcgtgcg aatcgcgtga tcgatcacgg ccttttcttc
     3841 ggtctgaatg ccggaaagat cgacgaccgt catggtgaat acggcgttgg cctgcgcgac
     3901 cgaatagacc cgcgcttcag cgcccgtacc gtccgcggtc tggtaggatt tggtttccac
     3961 caccggttcg gccggaaagg aaaccgcaaa cgcatattgc ggataggtgt attccttcca
     4021 gctctgcgcg gtggcgggag cggcggcgaa aaacgccagg gcggcaaaaa gcgcgatcaa
     4081 acgcatggct tcctcacatc acgattggtc tcagacggct tgccgccctc ccggcgcgtc
     4141 agtgaagcaa ctggaacgtc tgttggaaga aatgaatacc cgacgagccg atgggatcgt
     4201 tgttcacggc gggataggtg accttgagtt cgtagagctt gttgtcgatg aagaagatgg
     4261 cgtcggtata gctggtgccc gtggcatctt cgacgcggat ttcgcggccg taatttccat
     4321 cgatgcgcgc gcccgattca ttgtgggtca gcttgccgag gcccgacaga ttcgcgacgg
     4381 cttcctcgat ggtcttgtct ttttccgcgc gcgtgcgtgt gaaatcggcc accgccacgg
     4441 aatagatgac gccgccattg ttggtggaat agacgcgctg cttgacgatc ccatcgggtg
     4501 tctgcgccgt tttgtagtcc acggtttcaa cctgtggagg attgggatat tgcgccgcga
     4561 acccggattc ggggtaagaa aatagctgcc agccgccctc ggcggccaat gtgccgcctg
     4621 cgcaaaggac cgccgtaacc agaatgcaat atgcgcgcat atcagcgtcc ccacatccaa
     4681 gccggcgctt agcctgccat aaaatgccgg gtttggccta tcgcgaggcc gtgaacgcga
     4741 cgcttttaaa caggcttggt atgtgttatt ctaacaaata catatttgga ttgtaaaatg
     4801 acgaattccg ttctctctct cgggaccgac acgcgcacag cgctgcgccg gctggcggag
     4861 gcgctttcgc ttccccgtct cgaaccgggc tgggtctggc tcgcgggcgc cggaccgggc
     4921 gacccgggcc tcgcctcgct ccatacgttg catgccatcg ccgaggcgga cgtgatcctg
     4981 accgacgcgc tgatcgacaa gacattgctc gtgctggccc gccccgacgc tcaaatcgtc
     5041 gatgccggaa aacgcggcgg caagccgtcg gcggtccagg agacgatatc gcgccgtctg
     5101 attgcctatg cgcgcaaggg ggcgcgggtg ctgcggctga agggcggcga tcccttcgtg
     5161 tttggccgcg gcgcggagga agcgctggcg ctggcgcgcg cgggcgtccc gttccgggtc
     5221 atccccggca tcaccgcggg gatcggcggc ctcgcctatg ccggtattcc cgtcacccat
     5281 cgcgacacga accaggccgt caccttcatc accggcagtg gggccggggg tcgcgcgccc
     5341 gatctcgact gggcggcgat cgccaaaggc tcgcccacga tcgtgctcta tatggcgcgc
     5401 aggcatgcgg gcgagatcgc tgggcatctg gtgaacgccg gacgcgatgg caatgagccg
     5461 gcagccatcg tcagcaacgc ggcgcgcgac ggtcagacga cgatcgtgac gcgcctcgcg
     5521 gcgcttggcg aggccgccgc gctggccgaa acgcctgcca tcatcatcat cggcgagaat
     5581 gtgcgcctcg ctgccggtct ggactggctt ggcgctctct ccggacgtct gctcgatccc
     5641 gatccactcg gccgcgaacg cttgagcgac gcgggctgac cggcgttaac gaaggtttag
     5701 ccgcatccct cctacagtcg ctgccgggat ttgccggaca agagcgatgg cgcgcgcgca
     5761 atttcataag aaccagcggg tctatgtgcg gcccgtcgga acctgggcac tgatcgaacg
     5821 cctggtgccg caatggacca aaggcctcga agaacccttg cgcattttct acgacgtggg
     5881 gcttggccgt gatttcggcg ccgatgaatt ggaggcggag tcctccgttg cgccgccgga
     5941 ttccgacgag gaactttggc gggtcgtacg cgcgcccaac aaatggcagt cgccggccga
     6001 atccaccacc catccctatc cgggaactta tccggttatc atcacgggcg aagccgaatg
     6061 gggcggctgg cgcgtgccgg gtgcggaata tgcgctgtcg ccggaccgca tcgagtatca
     6121 ggcgcgcatg atcgcgagta tcccgcggct tgccgccatc gtgaagaagc tcagcgaatg
     6181 ggcggagggc actcccaatg gggatgttcc cctctcgttg cgcgacatcc tgcgcgagag
     6241 cgacgaggcg accgcctatc tcaccgacag cgtggcctaa acagcgtagg ctgacgcgat
     6301 tttcgcgtgg gcatctgacg cgatttacgg cagccttcct gacttacatc gcagccttcc
     6361 tgacttacat cattggcatc ggcgttatat ggtttgcgac gctcctcatc ccggatagca
     6421 atgaaaaccg ccgaaccacg cgccatacac cttagagatt acgccccccc gccctaccgc
     6481 attcccgaga tcgccctcga tttcaccttg gatggcaacg ccacgcgcgt gaccgcaacc
     6541 atgaaagtgg agcgccaggc cgaatccgcc gagcccctgg tgcttaacgg tcacgggctg
     6601 aagctgatct ccgtggcgcg cgacggcaag atgctgacgc ctcccggcta tgctgtggat
     6661 tcggacgggc tgacgctcgc ggatccgccc gcggcgttca cgcttcaaat cgtcaccgag
     6721 atcgcgccgg ccgcgaacac cgcgctcgaa ggtctctata tggcgggcgg aatctattgc
     6781 acgcaatgcg agccggaagg ctttcgcagc atcacctatt tcctcgaccg
//
LOCUS       DASYEG010000227         4749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4719241, whole genome shotgun sequence.
ACCESSION   DASYEG010000227 DASYEG010000000
VERSION     DASYEG010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4719241"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1347)
                     /locus_tag="VGM72_10025"
     CDS             complement(34..1347)
                     /locus_tag="VGM72_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265646.1"
                     /translation="MITRRAAAAGMLGMAASAPLARAAAPSWNVPPEGIGNRIKHVSW
                     SDQGGRLDGVQIMLNRRHLYIGHQFTDGFSVMDASDPRALKPVKYILTAPNTSTHHLQ
                     VANDILLVAEGANVMAMQSYDDARSYFENTLADSITKKVPFRAGLSIYDIKTDPASPR
                     EIAFLQIPGFGINRLWWTGGRYAYVAAHFDGFTDHILGIVDLQNITKPELVSKAWLPG
                     MNRAANEPNTTPKGKRYALHHMIVAGNRGYGAWRDGGFTIHDVGDPAHPKLLSHINWT
                     PPFAGGSHTPLPLPGRKLALVAEESNAEECAKGMFYTWIVDVRNDANPLPIGALPTPK
                     DRNYCAMGNFGPHNLHENRPGSFQSEETVFATYHNAGVRLFDIKDQFTPKEIAFWVPP
                     APKKLVDTHTGVALAPQSCDVYVTREGLMYVTDWNAGMHVLQYDG"
     gene            complement(1453..2205)
                     /locus_tag="VGM72_10030"
     CDS             complement(1453..2205)
                     /locus_tag="VGM72_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265647.1"
                     /translation="MSAPAFADAPQGFAGTLSGSYTDWKGSNDSANLWSTTGQAAFGL
                     GQYGMQDLAAEFDGGYHSLSNGSAGNLDIWNAGGNLFWAPAAPYRLGATLQYEDLSGG
                     GFGTHLWQYGAFGEYYFNPMITIGLNGGAWSGPQGTFGSGNSGGYVGGGATAYVMPDL
                     ALTGQMNYFSGSNAHLTSYSAQAEYLVSEATPVSVFGGYTFTDIPGASNHIDEWRIGV
                     KFYTNGNGTTLVDKHRNGALDDLVRPTLPMGE"
     gene            complement(2502..3548)
                     /locus_tag="VGM72_10035"
     CDS             complement(2502..3548)
                     /locus_tag="VGM72_10035"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4265648.1"
                     /translation="MVSSGNSRFVQDADHPGRSVLELAVALSDNPRTRPVIEGRVKFE
                     GLRLVPTTVHPSEMFWRQLRYGDFDVSEMSLSTLTILTSKGNRDWVGLPIYTMRKFFH
                     NEIVVRKDSGIETPANLKGKRVGVPEYQQTAAIWTRGVLQHEFGVHARDMTWWMERNA
                     DKSHGTSTGFKAPEGVDITPVPLNTNLGDMLLKGEIDAIVHYIANNNLVDRSRADLIA
                     DPRAKYLFPDREAESARYFKKTGIFPINHCMVIKRSVYEQHPWIALNLYSGFLAAMEQ
                     VRKDSLAWMALYYEMGLVEREVRNVVTKTPMPYGIKAARLVLEMVTQFVHEQGLCERR
                     VAVEELFAPNSLDL"
     gene            complement(3542..>4749)
                     /locus_tag="VGM72_10040"
     CDS             complement(3542..>4749)
                     /locus_tag="VGM72_10040"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY4265649.1"
                     /translation="LRRYWQPACLASELPEPDGAPLRVRLLGEDLIAFRDTSGKIGLV
                     DAYCPHRRAPLFFGRNEECGLRCVYHGWKFDRNGDCVDMPSEPAGTTLQTKVKLLAYP
                     AVEKAGAIWAYMGPKDLQPPEPDYEWTRAPEKHRFISKTFENCNFLQALEGGLDTSHS
                     SFAHNNFANDKSEPRQRDRSPRIDVERTDYGYYYVSTRNLGADGNYVRVYQYVMPAQQ
                     MRANNVGWFGGKNEVPRADGHIWVPIDDTTTHVYNWMCSYDDTVELTAEWIEARESGM
                     GRGSNDIIPGTFRLKRNQSNDYLIDREMQKTRNFTGIVGVNTQDFALQEGMGPVTDRS
                     KEFLAGSDKAVLAMRRLMLEAVDIAEKGGTPRGLDPATHRAIRPYDRILPPGEDWRMT
                     FKQELGAKW"
BASE COUNT          909 a         1411 c         1457 g          972 t
ORIGIN      
        1 cttgcagcct atcacacccg gcgagcgctg gcctcagcca tcgtattgca gcacgtgcat
       61 gcccgcgttc caatccgtca catacatcag gccttcgcgt gtcacataga catcacagga
      121 ctgcggcgcc agcgcgacac ccgtgtgcgt atcgaccagc ttcttcggcg cgggcggcac
      181 ccagaaggcg atctccttcg gcgtgaactg atccttgatg tcgaacaggc gcacgcccgc
      241 attgtggtag gtggcgaaga ccgtctcctc gctctggaac gagccggggc ggttctcgtg
      301 cagattgtgc ggcccgaaat tccccatcgc gcaataattg cggtccttgg gtgtcggcaa
      361 ggcaccgatg ggcagcggat tggcgtcgtt ccgcacgtcc acgatccagg tgtagaacat
      421 gcccttcgcg cattcttccg cgttcgattc ttccgcgacg agggcgagct tgcgccccgg
      481 cagcggcagc ggcgtgtgcg agccgccggc aaagggcggc gtccaattga tatgcgacag
      541 cagcttggga tgcgccggat cgccgacatc gtgaatggtg aaaccgccgt cgcgccatgc
      601 tccatagccg cgattccccg ccacaatcat gtgatgcagc gcgtagcgtt tgcccttggg
      661 cgtggtgttg ggctcattgg cggcgcggtt catgccgggc agccacgcct tcgagaccag
      721 ctccggcttg gtgatgttct gcagatccac gatgcccagg atatggtctg tgaagccgtc
      781 gaaatgcgcc gcgacatagg catagcgtcc gccggtccac cacagccggt tgatgccgaa
      841 gcccggtatc tgaagaaagg caatctcgcg cggggaagcg ggatcggtct tgatgtcata
      901 gatcgaaaga ccagcgcgga acgggacctt cttggtgatg gaatcggcga gcgtgttctc
      961 gaaataggaa cgcgcgtcgt cgtaagactg catcgccatg acattggcgc cttcggcgac
     1021 gagaagaatg tcgttcgcca cctgcagatg atgcgtgctc gtgttgggcg cggtgaggat
     1081 gtatttcacc ggtttcagcg cgcgcgggtc tgaggcatcc atcaccgaga agccgtcggt
     1141 gaattggtgg ccgatgtaca gatgccggcg gttcagcatg atctgcacgc catcgagccg
     1201 gccgccctgg tcgctccagg agacatgctt gatgcggttg ccgatgccct ccggcggtac
     1261 gttccaggac ggcgcggcgg cacgcgccaa tggagccgag gcagccatcc ccagcattcc
     1321 ggccgcagcc gcgcgtcgcg tgatcatggt gaaatcccat cccgtcgtcg cacaagctta
     1381 gcaggcgcca cgaaggggtg gaacgaaaac ctgccccctc ccaacttcga gagggggcag
     1441 gaaacgccgt gattattcgc ccatcggaag cgtcggacgc acaagatcgt cgagcgcgcc
     1501 gttgcggtgt ttgtcgacaa gggtggtgcc gttgccattg gtgtagaatt tcacgccaat
     1561 gcgccactca tcgatatggt tgctggctcc cggaatgtcc gtgaaggtgt agccgccgaa
     1621 aaccgaaacc ggcgtggctt ccgaaaccag atattccgcc tgcgcgctat agctggtcag
     1681 atgcgcattg ctgccgctga aataattcat ctgccccgtc agcgcgagat cgggcatcac
     1741 ataagcggtg gcgccgccgc cgacataacc gccgctattg cccgaaccga aagtgccttg
     1801 agggccactc caggcgccgc cgttcaagcc gatcgtgatc attgggttga agtaatattc
     1861 accgaaggcg ccatactgcc acagatgtgt gccgaagccg cctccgctca agtcttcgta
     1921 ttgcagcgtt gcgcccaaac ggtatggcgc agccggagcc cagaagaggt tgccgcccgc
     1981 attccaaatg tcgagattgc ccgcgctgcc attcgagagg ctgtggtagc caccgtcgaa
     2041 ttccgcagcc agatcctgca taccatattg accgaggccg aacgcggcct ggccagtagt
     2101 gctccaaaga ttggcgctgt cgttcgagcc tttccaatcg gtgtagctgc cgctgagcgt
     2161 accggcgaag ccttgcggtg catcggcgaa agccggcgcg gacatggcaa ggctcatcgc
     2221 agccgtgcaa gcgccggcca aaagcaagtt taaacgagac ataagttttc ccttcaattt
     2281 ccggagatgt ttttgcgcaa caccgatgaa cgctccggac gaatcgctca tcgccggtgt
     2341 gtatagtcgg cggaggttcc ggcggttgcc ttaaatcgcc tccagatcgg agattggaat
     2401 catgtgtgac gaatttgcat cgcccaatga tccgcaggcg gataaatcat ccgacagtat
     2461 cgacaatttt gtgtgatgca ggattgaacg cgagcgcagc cttacaggtc tagcgagttt
     2521 ggcgcgaaca gttcttcgac tgcaacccgg cgttcgcata ggccttgttc atggacaaat
     2581 tgtgtgacca tttcgagcac gaggcgcgcg gccttgatcc cataaggcat cggggtcttc
     2641 gtcaccacgt tgcgtacctc acgctccacc agccccattt cgtaataaag cgccatccac
     2701 gcaagggaat ctttgcgcac ctgctccatc gcggcaagga acccggaata gaggttgagc
     2761 gcgatccagg gatgctgttc gtagacggag cgtttgatca ccatgcaatg gttgatcgga
     2821 aaaatgccgg ttttcttgaa atagcgcgcc gattccgcct cgcggtccgg gaatagatat
     2881 ttggccctgg gatcggcgat gaggtccgcg cggctgcgat cgaccagatt gttgttggcg
     2941 atgtaatgca cgatggcgtc gatttcgcct ttcagcagca tgtcgccgag attggtgttg
     3001 agcggcaccg gagtgatgtc aacgccttca ggcgccttga atcccgtgga tgtgccgtgg
     3061 ctcttgtcgg cgttgcgttc catccaccat gtcatgtcgc gcgcatggac gccgaattca
     3121 tgttgcagca cgccgcgcgt ccagatcgcc gccgtctgct gatattccgg cacgccgacg
     3181 cgcttgcctt tcaggttcgc gggtgtctcg atcccggaat ctttgcgcac cacgatctca
     3241 ttgtgaaaga atttgcgcat tgtgtagatg ggcagcccca cccaatcgcg attgcccttg
     3301 gacgtcagga tggtcagcgt cgagagcgac atttccgaaa cgtcgaaatc gccgtagcgg
     3361 agctggcgcc aaaacatttc ggacgggtgc acggttgtcg gcaccagccg caggccttcg
     3421 aatttcacac ggccctcgat caccgggcgc gtgcgcggat tgtcggagag cgccaccgcg
     3481 agttcgagca ccgaacgccc gggatgatcc gcgtcctgca cgaaacgact gtttcccgaa
     3541 ctcaccattt ggcccccaat tcctgtttga acgtcatgcg ccaatcttcg cccggcggca
     3601 aaatccggtc ataaggacga atggcgcgat gggtggcggg atcgagcccg cgcggcgtgc
     3661 cgcctttttc cgcgatgtcg acggcttcca gcatcagccg ccgcatggcg aggaccgcct
     3721 tgtcggagcc ggcgaggaat tccttcgagc ggtccgtgac cggacccatg ccttcctgca
     3781 gcgcgaagtc ctgcgtgttg accccgacaa taccggtgaa attgcgcgtc ttctgcatct
     3841 cacggtcgat gagataatcg ttggattggt tgcgcttcag ccggaaggtt ccggggatga
     3901 tgtcgttcga gccgcgcccc atgcccgatt cgcgggcttc gatccattcc gctgtcagct
     3961 cgacggtgtc gtcgtaggag cacatccaat tgtagacatg ggtggtggta tcatcgatgg
     4021 gcacccagat atgtccatcg gcgcgcggca cttcgttctt gccgccgaac cagcccacat
     4081 tgttggcgcg catctgctgt gccggcatta catattggta cacgcgcaca tagtttccat
     4141 ccgcgcccag attgcgcgtg gagacgtaat aataaccata gtccgtgcgc tcgacatcga
     4201 tcctgggtga gcggtcgcgc tggcgcggtt cggatttgtc attggcgaaa ttgttgtgcg
     4261 cgaaggagga atgcgaggtg tcgaggccgc cttccagcgc ctggaggaaa ttgcagttct
     4321 cgaaggtctt ggaaatgaag cggtgctttt cgggggcccg cgtccattcg taatcgggct
     4381 cgggcggctg cagatccttg ggccccatat aggcccagat cgcgccggcc ttttcgaccg
     4441 ccggataggc gagtaatttc actttagtct ggagcgtcgt tccggcgggc tccgacggca
     4501 tgtcgacgca atccccgttc cggtcgaact tccagccatg atagacgcag cgcaggccgc
     4561 attcctcgtt gcgcccgaag aacagaggcg cgcgccggtg cggacaatag gcatccacca
     4621 gaccgatttt gccggacgtg tcgcgaaagg cgatgaggtc ttcgcccagc aggcgcacgc
     4681 gcaaaggcgc gccgtcgggt tcgggaagct cggaggcaag gcatgccggc tgccaatagc
     4741 ggcgcaaca
//
LOCUS       DASYEG010000228         2355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4137829, whole genome shotgun sequence.
ACCESSION   DASYEG010000228 DASYEG010000000
VERSION     DASYEG010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4137829"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="VGM72_10045"
     CDS             complement(<1..1010)
                     /locus_tag="VGM72_10045"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY4265650.1"
                     /translation="MMPESTSNPEIPENKPAPEGQLSAVLPLRDIVVFPHMIVPLFVG
                     REKSVRALEEVMNEEKQILLLTQKNAGEDDPSPDALYRVGTLATVLQLLKLPDQTVRV
                     LVEGKARARVKTFSPRTDFFQAMIEPIPEESGNAQEIEALVRSVKTNFESYIKLNKKV
                     PAETLASVAQIDDPAKLADTVASHLTVKIPDRQQLLETLNTVERLERVLALIEGEIGV
                     LQVERKIRSRVKRQMEKTQREYYLNEQLKAIQKELGEGEEGRDELAELEERIKKTKLS
                     KEAREKAAAEVKKLRSMSPMSAEATVVRNYLDWMLSLPWGKKSKVKKDISVAEHVLHE
                     DH"
     gene            1454..>2355
                     /locus_tag="VGM72_10050"
     CDS             1454..>2355
                     /locus_tag="VGM72_10050"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEY4265651.1"
                     /translation="MFYSAVGRDLTLYDMDVEAATLTRRSTVTLPGNVQYAWPHPSKR
                     YFYVISTNGEPGGGDAPKGDTHVATAFRIDPATGALTKHGPEPRLPSRPIHTSVDAAG
                     KFLLIAFNEPSNVIVHRINDDGTIGALVTQKQKLDTGIYGHQIRTTPGNRTAILITRG
                     NNATAAKPEDPGAIKLFGFNDGVLTNLESVAAGNNGYGFGPRHHDYHPSKPWVYVSIE
                     RQNQIYAFAMNPDGSLAPKPLFIKGTLAGPRVPEAAQGAGTIHIHPNGKFAYIPNRAS
                     GTVKFQGKDVWAGGENNIAVFALN"
BASE COUNT          478 a          710 c          678 g          489 t
ORIGIN      
        1 taatggtcct cgtgcagaac gtgctcggca acactgatgt cctttttgac tttggacttc
       61 ttgccccaag gcagtgacag catccagtcg aggtaattgc gcacgacggt ggcttccgcc
      121 gacatcgggc tcatggaacg cagcttcttg acctcggcgg cggctttttc gcgcgcttcc
      181 ttggagagct tggtcttctt gatgcgctct tcgagttcgg ccagctcgtc gcggccttct
      241 tcgccctcgc cgagctcttt ctggatcgcc ttgagctgct cgttcaaata atattcgcgc
      301 tgcgtcttct ccatctgacg cttcacgcgg ctgcggatct tgcgttcgac ctgcagcacg
      361 ccgatctcgc cttcgatgag ggcaagcaca cgctccagcc gctcgaccgt gttgagcgtt
      421 tccagcaatt gctggcgatc cggaatcttc accgtcaggt gcgaagcgac cgtatcggca
      481 agcttcgccg gatcgtcgat ctgggccacc gaggccaggg tctcggccgg cactttcttg
      541 ttgagcttga tatagctctc gaaattggtt ttcaccgaac gcaccagggc ttcgatttcc
      601 tgggcgttgc ccgactcttc cggaatcggc tcgatcatcg cctggaagaa atcggtgcgc
      661 ggcgaaaagg tcttgacgcg cgcacgcgct tttccttcga cgagcacgcg gaccgtctga
      721 tcgggcaact tcagaagctg aagcacagtg gcgagcgtgc cgacacgata aagcgcgtcg
      781 ggggacggat cgtcctcacc ggcgttcttc tgcgtcagca gaaggatctg cttttcctcg
      841 ttcatgacct cttcgagagc acgaaccgat ttctcacggc cgacgaaaag cggcacgatc
      901 atgtgcggga agacaacaat gtcgcgaagc ggcaggacag ccgacaactg gccttccggt
      961 gcgggtttgt tttctgggat ttcaggattc gacgttgatt caggcatcat gtgctcctta
     1021 cccgggaaca ctagcacgca agccttaaac ctttgcctgt ggcatcatgg cgggcggggt
     1081 ttccatgccc cgaaatgggc tcatggacaa atcatggtgc ccttctgggc caccacgcaa
     1141 acactaaggt gggctgcgaa gaatgcggat tcaaggttta tccgcgtgat cgactagaaa
     1201 accaggggat ttctaggttt atgttcagct acagcttgga caatttcctc gtttcgcgcg
     1261 cactttgcgg cggacaggtc ctttgccaat atgggcatgg ggcaatcgcg gttaaacacg
     1321 ttacgcgcca acctccaacc gaatcacgat ttgcggggca aacatgatca accgccggac
     1381 tttcgctacg ctcgttgcgg gcagcatgct cctcgcgaaa aacacatggg ggcagacggc
     1441 cggaggaaaa accgtgttct attccgctgt cggccgggac ctgacgctct atgacatgga
     1501 tgtggaagcg gcgacgctga cgcggcggag caccgtgacg ttgccgggca atgtgcaata
     1561 tgcctggcct cacccttcca agcgctattt ctacgtgatc tcgaccaatg gcgagcccgg
     1621 cggcggcgat gcgccaaagg gagacaccca tgtcgccaca gcgtttcgta tcgatccggc
     1681 gaccggcgcg ctgaccaagc acgggccgga accgagactg ccgtcgcgtc ccatccatac
     1741 cagcgtcgat gcggccggga aattcctgct gatcgcgttc aacgaaccca gcaacgtcat
     1801 cgttcatcgc atcaatgacg acggcaccat cggcgctctg gtgacccaga agcagaaact
     1861 cgataccgga atttacgggc atcagattcg gaccacgccg ggaaaccgca cggcgatcct
     1921 gatcacgcgc ggcaacaacg cgacagcggc caagccggaa gacccgggcg cgattaagct
     1981 gttcggattc aacgacggtg tcttgaccaa tctggaatcg gtggcggcgg gcaacaatgg
     2041 ctatggcttt ggcccgcgtc atcacgatta tcatccctca aaaccgtggg tttacgtttc
     2101 catcgaacgg cagaaccaga tttacgcctt cgcgatgaac cccgacggct cgctggcgcc
     2161 caagcccctc ttcatcaagg ggacgctcgc ggggccgcgc gtgcccgagg ccgcgcaagg
     2221 agccggcacc atccacatcc atcccaacgg caaattcgct tacatcccga acagggcatc
     2281 gggcacggtg aaatttcagg gcaaggatgt gtgggccggc ggcgaaaaca atatcgcggt
     2341 gttcgcgctc aacga
//
LOCUS       DASYEG010000229         5315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1239471, whole genome shotgun sequence.
ACCESSION   DASYEG010000229 DASYEG010000000
VERSION     DASYEG010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239471"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VGM72_10055"
     CDS             <1..724
                     /locus_tag="VGM72_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY4265652.1"
                     /translation="QPKVNLRTGEIIGVEGLIRWHHPERGLVPPVQFIPVAEDCGFIV
                     PIGQWVLREACRQGRAWRDSGLPAIGIAVNISAAELRTRNFVANVQAILKETRFEPRD
                     LELELTETFLMQDLESTAAVLRALKEIGVRLALDDFGTGYSSLSYLRRFPIDTLKIDR
                     SFVCDLTTDAGDASIVDAVINMGKSLHMAVVAEGVETPEQLALLQQQGCTEGQGYFFS
                     RPVSAERTTQLLERSAGKAVFS"
     gene            753..1073
                     /locus_tag="VGM72_10060"
     CDS             753..1073
                     /locus_tag="VGM72_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265653.1"
                     /translation="MRKSRAIGSNLVSAPVMRTTGEISFSTSLSGNCTKEFHAAAMRG
                     VCVMPLYEFRIRSQGSTIWIVEHYCRDDLDALEKAASDFGVRVWHNGHPVAHADKKTP
                     RKPR"
     gene            1133..1534
                     /locus_tag="VGM72_10065"
     CDS             1133..1534
                     /locus_tag="VGM72_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4265654.1"
                     /translation="MANILLVDDEALVVETLSNAMKNKGHSVVTASNGIEGLKRFGEG
                     DCNLVITDIVMPDMDGMAMIMEIRHSAPAAKIIAISGGGRSGNVEFLKMAAKLGAMAT
                     LRKPIRLAEFFKTLDECLSERHGTAMAVLQS"
     gene            1578..2891
                     /locus_tag="VGM72_10070"
     CDS             1578..2891
                     /locus_tag="VGM72_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265655.1"
                     /translation="MNATTTVSGKQDSLRQSTSAASKMTVLGLDSIRARTGDQWKKRS
                     EKVHSYFTMLFQREMRPGDTFHRVDELSYLVIFRELSVADAQAKCLAIADTVSRRLFG
                     EDTGAVSIRSVIGAVSNRLLTEDVEEQAAIQAFLEAEGTEIITTSEGPEILERTVDTR
                     IEPPKELSAPPLPPAQHLNVTFGPDHDGSQALLENEFAFKYRPFWDAARKVVLMYVCQ
                     PAPAAPPKDASPHSFAYGLCVSPERNESSRLDLLVLEEAAQRVEFLRRSGYRILAACP
                     VHFSTIAGARSWGEYLRALTRLRADVVRDIVFLIMGIDGSIPGIRLVQEIPKLSARTK
                     LVFAAIKYGPGVVRRFANTGIHAIGVELPRPEGSDRPLLTAVDALARDAQALGIESFV
                     LGARNRSTIVGAIASGVRYLEGLAVSPPVFEPRHAFAQEIADLYR"
     gene            complement(2954..4513)
                     /locus_tag="VGM72_10075"
     CDS             complement(2954..4513)
                     /locus_tag="VGM72_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEY4265656.1"
                     /translation="MRGYRNFGPGAASWTKRFLHEKQGSTAIIFALSSFVLLSSVGVG
                     VDLARAMMVKSRLSSALDAAALAVGTSNGLDTPHLQTLAQQYFDANYPKTQIGVANPV
                     TVAVNGQHIALGVGASMPTSLLEIMGIHNIDLSVTNEVTRAMTKLRVALVLDNTGSMS
                     QTDATGTSKISALKTATHQLLTQLQGTAANPGDVQVSIVPFSLDVNMGTAVANQTWID
                     WTDFDAAPPNSKPLESGAIPSNVGPGSSCPFATNSSPYGYGCLYLSSGNIAADGKIYP
                     GVDNGKYNAGRKGHHYNGYFDSVKTDPSCTSNCNYSHSWHTYNQAAKADLPLHGAWTG
                     CFMDRAQNYDTTNATPSTGIPASLFPAEISDSCPPVALNGLIYDWSALNTKVDLMTPN
                     GSTNQTIGLAWGWQALTSTNPLNAPAQDPTVQNVIILLSDGMNTQDRFGGDGSSTSSA
                     VDSRMALACANAKAANVRIYTVLVMAGNSSVLQSCATDTSKYFSLTTAGQIVTAFNSI
                     GTELANLHLSR"
     gene            complement(4733..>5315)
                     /locus_tag="VGM72_10080"
     CDS             complement(4733..>5315)
                     /locus_tag="VGM72_10080"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4265657.1"
                     /translation="LRLTFHRDNDRPLLTNGAADWLWPEAERLGMPVMIHAPERLPVI
                     GAIAERHPDLKLIVDHMGFARETMDEEAKAGASRILALARHPNISVKMSALPCYSTQA
                     YPFRNLHEPLKRIIGGFGVRRSFWGSDLSRLPAGCSYRQAVTMFTRELDFLSGSDLEW
                     VMGRGIMECLSWADPPLEIKSVCIVAGGQPCGV"
BASE COUNT         1066 a         1513 c         1666 g         1070 t
ORIGIN      
        1 tcagccgaag gtgaacctga ggacgggtga gatcatcggg gtcgaggggc tcatccgctg
       61 gcatcatccc gaacgcggcc tggtgccgcc ggtgcaattc ataccggtcg cagaagactg
      121 cggcttcatc gttcccatcg gccaatgggt gctgcgcgaa gcctgccgcc aaggcagggc
      181 ctggcgggat tcgggcctgc ccgcgatagg tatcgcggtg aacatctcgg ctgcggagtt
      241 gcgcacccgg aacttcgtcg cgaacgtgca agcgatcctg aaggaaacga ggtttgaacc
      301 gcgcgatctg gaactggaac tgacagagac cttcctgatg caggatctgg agtcgaccgc
      361 cgccgtgctt cgagccctga aggaaatcgg cgtgcggctc gccctcgacg attttggaac
      421 cggctattct agcctcagct atctgaggcg atttccgatc gacaccttga agatcgatcg
      481 ttcgttcgtg tgcgatctca ccaccgacgc cggcgatgcc agcatcgtgg atgcggtgat
      541 caacatgggg aaaagcttgc atatggcagt ggtggccgag ggtgtggaaa cccccgagca
      601 gctcgcgctt ctccagcagc agggttgcac cgaggggcaa ggctatttct tcagccgtcc
      661 ggtatcggcg gagaggacca cccagcttct ggagcgtagc gccggcaaag ccgtgttctc
      721 ttgatgaagg agccggcggg acaaggatct caatgcgcaa atcgcgcgcg atcggaagca
      781 atcttgtgag cgcgccggtg atgagaacaa cgggggaaat cagcttttcg acatcattgt
      841 ctgggaactg cacgaaagaa tttcatgcag cagcaatgcg gggagtgtgt gtgatgcctt
      901 tatatgaatt ccgtatccgt tcacaaggct cgaccatttg gatcgtcgag cattactgcc
      961 gcgatgatct cgacgctttg gagaaagcgg catccgattt cggggtcagg gtctggcata
     1021 acggccatcc ggtcgcgcat gccgataaga agacaccgcg aaagccgcga tagatttcaa
     1081 tgccgattga gatgttcgcc agccgggggc atggcgagac ggaaggtgaa agatggcgaa
     1141 tattcttctg gtggatgacg aggcgctggt ggtggagacc ttgtcgaacg ccatgaaaaa
     1201 caaaggccac agcgtcgtca cggcctcgaa cggaatcgag gggctgaagc gatttggcga
     1261 aggcgattgc aatctcgtca tcaccgatat cgttatgccg gatatggacg gaatggcgat
     1321 gatcatggaa atacgccaca gcgcgcctgc cgccaagatc attgcgatct ccggcggcgg
     1381 ccgaagcggt aatgtcgagt tcctgaagat ggcggcgaag ctcggcgcca tggccacgtt
     1441 gcgaaaaccc atccgtctgg ccgaattctt caagaccttg gacgaatgtc tgtccgaacg
     1501 tcacggcacc gcgatggccg tattgcagag ttgatcatca gccgcgcgcc acagtgcgaa
     1561 aaatgccgga cgccatcatg aacgcaacga cgaccgtgag cggaaaacag gacagcctca
     1621 ggcaatccac atccgcggcg agcaagatga cggtgctggg attggacagc atccgcgcgc
     1681 gtacaggcga tcaatggaag aagagatcgg agaaggtcca ctcctatttc accatgctgt
     1741 ttcagcgcga aatgcggccg ggcgatacgt tccatagagt cgacgaattg tcctatctcg
     1801 tcattttccg cgaactttcg gtcgccgacg cacaggcgaa atgtcttgcg atcgccgata
     1861 ccgtaagccg gcgtcttttt ggcgaggata ccggcgcggt ctccatccgc tccgtcatcg
     1921 gggcggtcag caaccggctc ctgacggagg atgtggaaga acaggcggca atccaggcct
     1981 ttctggaagc cgaagggacc gaaatcatca caacctccga aggccccgaa attttagagc
     2041 ggacagtaga cacgaggatc gaaccgccga aagaactgag cgcgcctcca cttccgccgg
     2101 cgcagcatct gaatgtgacg ttcggtcccg atcacgatgg ctcgcaggcg cttctggaaa
     2161 atgagttcgc cttcaaatac cggccgtttt gggatgcggc gcgcaaagtc gtgctgatgt
     2221 acgtttgtca gcccgcgccc gcggcgccgc caaaagatgc gtcgccgcat tcgttcgcat
     2281 atggcctttg cgtttcgccg gagcgcaacg aatcaagccg tctcgacctt ttggtcctgg
     2341 aagaagccgc ccagcgcgtg gaattcctgc gccgatcggg ataccggatt ctggcggctt
     2401 gcccggttca tttctcgacc atcgcgggcg cgcgaagctg gggggaatat ttgcgcgcgc
     2461 tgacacggct tcgggccgat gtcgttcgcg atattgtgtt tctcatcatg ggaattgacg
     2521 gcagtattcc aggcatcagg cttgtccagg agattccgaa actgtccgcg cgcacgaaat
     2581 tggtgttcgc ggcgatcaaa tatggccccg gagtcgtgcg gcgattcgcg aacaccggga
     2641 ttcacgccat cggagtcgaa ctgccccggc ccgaagggtc cgaccggccg ctgctgacgg
     2701 ctgtggacgc gctcgcgcgc gatgcgcagg ctctggggat cgagagtttc gtgctcggtg
     2761 cgcgaaaccg cagtacgatt gtcggcgcga tcgcttcggg ggtgcgttac ctggaaggtc
     2821 tcgcggtttc gccacccgtg tttgaaccgc gtcacgcctt cgctcaggaa atcgcggatc
     2881 tttatcgctg atacaaaaaa cccctccggt gaaggagggg cagtcgcgct cgacgaaaag
     2941 aatgacgtgc ggctcagcgt gacagatgca gattggcgag ttcggttccg atgctgttga
     3001 acgccgtgac gatctggcca gccgtggtga gggagaaata tttgctcgtg tccgtggcgc
     3061 aagattgcag gaccgaggag ttacccgcca tcaccagaac cgtgtagatg cgcacattgg
     3121 cggccttggc attggcgcag gcgagcgcca tgcgggaatc gaccgcggaa ctcgtgctcg
     3181 atccatcgcc gccgaagcgg tcctgcgtgt tcatgccgtc gctcaagagg atgatcacgt
     3241 tctggacggt cggatcctgg gccggcgcgt tcagcggatt ggtcgaggtc agcgcctgcc
     3301 agccccaggc caatccaatc gtctggttgg tcgagccgtt cggcgtcatc aggtcgactt
     3361 tcgtattgag cgcggaccaa tcgtagatga ggccgttcag cgccaccggc gggcatgagt
     3421 cgctgatttc cgccgggaac aggctcgccg gtatgccggt ggaaggcgtt gcattggtcg
     3481 tgtcgtaatt ctgcgcgcgg tccatgaagc acccggtcca cgcaccgtgc aggggcagat
     3541 ccgccttggc cgcctgattg taagtgtgcc agctgtggct gtagttgcag ttgcttgtgc
     3601 agctaggatc ggttttgacg ctgtcgaaat agccattgta gtgatggccc ttacgtccgg
     3661 cgttgtattt gccgttgtcg acgcccggat agattttgcc gtccgccgcg atgttgccgc
     3721 tcgaaaggta cagacagccg taaccatatg ggctggagtt ggtcgcaaat gggcagctcg
     3781 atcccggccc tacgttgctg ggaattgccc cggattcgag tggcttcgag ttgggcggcg
     3841 ctgcgtcgaa atccgtccaa tcgatccatg tctggtttgc gaccgcggtg cccatgttga
     3901 cgtcgagcga gaacggaacg attgacactt gcacgtcgcc gggattagcc gcagtaccct
     3961 gcaactgcgt caacaattga tgcgttgccg ttttcagcgc cgagatcttg ctcgttcccg
     4021 tggcgtctgt ctgtgacatc gacccggtat tgtccagcac aagcgccacg cgcagcttgg
     4081 tcatcgcgcg cgtcacttcg ttggtcaccg acagatcgat attgtggatg cccatgattt
     4141 ccagcaggct tgtcggcatc gatgcgccga cacccagggc aatatgttgg ccgttgacgg
     4201 ccacggtgac gggattggcc actccgatct gggtcttcgg gtagttggca tcgaaatatt
     4261 gctgggccag ggtttggaga tgcggggtgt ccagcccatt tgaggtgccc accgcgaggg
     4321 ctgccgcgtc gagcgcgctg gaaagccgtg acttcaccat catggctcgc gccaaatcga
     4381 cgccgacacc cacgctggaa agcaggacga aggagctgag cgcaaaaatg attgccgtgc
     4441 tgccctgctt ctcgtggaga aaccgcttcg tccagcttgc ggctcccggg ccgaaattgc
     4501 gatatccgcg cattgtcgtg actgcccttc cgaggtgaac atccaggagt tcatcgcgcg
     4561 tccgtccggc gcggattgga actttcacag ggtcgctacg cacgacctgt gccagagaag
     4621 aatctcaaat attttcagtt atttggccgc taaaatgcgg catcgcgcac gcggcgcgtc
     4681 tcataacggc cccccgcacg tccaatttcg ggacgttgtg gggacgttgc tttcaaacgc
     4741 cgcaaggctg cccgccggca acgatgcaca cgctcttaat ctcaagcggc gggtcggccc
     4801 agctcagaca ttccatgatg ccgcggccca tcacccattc gagatcgctg ccggaaagga
     4861 aatcgagttc gcgggtgaac atcgtcaccg cctgccggta cgagcatccc gcggggaggc
     4921 gcgaaagatc gctgccccag aatgaacgcc gcacaccgaa gccgccgatg atgcgtttta
     4981 aaggctcatg cagattgcga aacgggtagg cctgggtgga atagcagggc aatgccgaca
     5041 ttttcaccga gatgttcgga tgccgcgcca aggcgagaat gcggctcgcc ccggctttgg
     5101 cctcttcatc catcgtctcg cgggcaaatc ccatgtgatc gacgataagc ttcagatcgg
     5161 gatgacgttc ggcgatcgcg ccgatcaccg gcaggcgctc gggcgcatgg atcatcaccg
     5221 gcattcccaa gcgttccgct tccggccaca gccaatccgc cgcgccattg gtgagcagcg
     5281 ggcggtcatt gtcgcggtga aaggtcaacc tcagc
//
LOCUS       DASYEG010000230         4437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1822058, whole genome shotgun sequence.
ACCESSION   DASYEG010000230 DASYEG010000000
VERSION     DASYEG010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822058"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..734
                     /locus_tag="VGM72_10085"
     CDS             141..734
                     /locus_tag="VGM72_10085"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004938990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEY4265658.1"
                     /translation="MLLLIDNYDSFTYNLFHYLGELGEEVLVKRNDQISVQDALAMSP
                     QSIVLSPGPCDPGRAGICLALIDAAAGRVPILGVCLGHQAIGQAYGGRIVRAPEPMHG
                     KLSAIHHTGKSVFRGLEDGFPATRYHSLVIERSSMPAALEITAETDDGIIMGVMHKSH
                     PVHGVQFHPESIASAHGHALLQNFLKLAWTTPRVSAA"
     gene            731..1762
                     /gene="trpD"
                     /locus_tag="VGM72_10090"
     CDS             731..1762
                     /gene="trpD"
                     /locus_tag="VGM72_10090"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEY4265659.1"
                     /translation="MSKPQGEFATFLKRVATGEHLAASESASAFAAMMAGSVSEMRMA
                     AFLTALALRGPSVAEITGAARAMREAMTPVEAPAGAMDVCGTGGDSAGTLNVSTAAAF
                     VVAACGVPVAKHGNRAMTSRTGAADVLEALGVPINHDAAAAKRSLIKPGFAFLFAPAY
                     HPAMKHVGPVRKELAFRTVFNLLGPICNPARVRRQLIGIFAEEWLEPVAHVLADLGTE
                     KAWVVHGTDGLDEMTTTGVTRVAILDHGQVTLREIAPEDAGLKRGPLTALKGGSAEDN
                     ARAIRDLFAGSRNAFRDIVLLNAGAALVVADKAKTIGEGVAAAAEAIDKGHAASTLTQ
                     SRISERAGV"
     gene            1767..2564
                     /gene="trpC"
                     /locus_tag="VGM72_10095"
     CDS             1767..2564
                     /gene="trpC"
                     /locus_tag="VGM72_10095"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001370.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEY4265660.1"
                     /translation="MSILERIAAYKREEVAAAKKERPLKALMADANEAPAPRGFRAAL
                     EAAKAARHPGLIAEIKKASPSKGVIRADFDPEVIARDYERGGATCLSVLTDTPSFQGA
                     DDDLMMARQATHLPVLRKDFMLEPYQVVQSRALGADCILVILAMLSDAMATRLIETAE
                     EWGMDALVEVHDENDLKCALALDAELIGINNRNLATFVTDIGTAIRLKPLIPADRHVI
                     AESGLSTPGDLTRLAAAGITSYLIGESLVRADDVEAATHALLSGVKL"
     gene            2561..3028
                     /gene="moaC"
                     /locus_tag="VGM72_10100"
                     /pseudo
     CDS             2561..3028
                     /gene="moaC"
                     /locus_tag="VGM72_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
     gene            3346..>4437
                     /gene="glp"
                     /locus_tag="VGM72_10105"
     CDS             3346..>4437
                     /gene="glp"
                     /locus_tag="VGM72_10105"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEY4265661.1"
                     /translation="MISVNEAVARIARGLTRLSSESVMVAEAAGRVLAEDAVARLDHP
                     PTPMSAMDGYAVRRADARAGAEFSIIGEAPAGRPFPGRIGEGQAVRIFTGGVVPEGAD
                     AVVIQEDTVPLASNDGVVLKVRITETPYPDENVRPRGLDFRKGEILLPRGHRISPRDV
                     ALIAAGDLAKVSVIRKPCVALVATGDELSRPGEPRKDGGIVASSIYALQAMVRQWGGE
                     AIDAGILPDRPEAFAALPEATKNADLVVTQGGASVGDHDLVQSALKPFGFTLDFWRIA
                     MRPGKPLIFGRLRDTPFIGLPGNPVSAMVCAVLFIQPAIAAMLGMTYEPPIVKARLGS
                     ALRANGRRQDYIRTRLVRHGGILTAEPFAL"
BASE COUNT          818 a         1395 c         1443 g          781 t
ORIGIN      
        1 acaacccaag aagccttgct aaagcattgt tttcacatat taaatgaagg gcttacgttc
       61 ctgctgcgtt tgcgcgaaaa tgagcttcga aacaggcgcg ctcccgtggc gcgcggacac
      121 accgaggaat acgcggctcc atgctgcttc tgatcgataa ttacgacagc tttacctaca
      181 atcttttcca ttatctcggc gagcttggcg aagaggtctt ggtcaagcgc aacgaccaga
      241 tcagcgtcca ggacgcactg gccatgagcc cgcaatccat cgtgctgtca ccggggccct
      301 gcgatcccgg ccgggccggc atctgccttg ctctgatcga cgccgcggca ggcagggtac
      361 cgatccttgg cgtctgccta ggccatcagg cgatcggcca agcctatggc ggcaggatcg
      421 tgcgtgcgcc cgagcccatg cacgggaagc tttcggccat ccaccatacc ggcaagagcg
      481 tctttcgcgg attggaagat gggtttcccg ccacgcgcta tcattcgctc gtgatcgagc
      541 gctccagcat gccggctgct ctggaaatca ccgccgagac cgacgatggg atcatcatgg
      601 gggtgatgca caagagccat cccgtccacg gcgtgcagtt ccatcccgaa agcatcgcat
      661 cggcccacgg tcacgcgctg ctgcagaatt ttctgaaact ggcgtggacc acaccccgcg
      721 taagcgcggc atgagcaaac cccaggggga attcgccacg ttcttgaaac gggtggcgac
      781 gggcgagcat ctcgccgcaa gcgaaagcgc aagcgctttt gccgcgatga tggcgggcag
      841 cgtcagtgaa atgcgcatgg ccgcgtttct gaccgcgctt gcgttgcgcg gaccatcggt
      901 cgccgagatc accggcgcgg cacgcgcgat gcgcgaggcc atgacccctg ttgaggcccc
      961 ggcgggcgcg atggacgtct gcggcaccgg aggcgattcc gcgggcacgc tgaacgtctc
     1021 gaccgcagcg gcgttcgttg tcgcggcttg cggcgtgcct gtcgccaagc acggcaatcg
     1081 cgcgatgact tcacgcaccg gcgccgccga cgtgctggag gctttgggcg tgccgatcaa
     1141 ccacgatgcc gccgccgcca agcgctccct gataaagccc ggtttcgcct ttctgtttgc
     1201 gcccgcctat catccggcga tgaagcatgt cggcccggtg cgcaaggaat tggcgttccg
     1261 cacggtcttc aatctgctgg gaccgatctg caatccggca cgcgtgcgcc gtcagctgat
     1321 cggaattttt gccgaggaat ggctggagcc ggtggcccat gtgctggccg atctgggcac
     1381 cgaaaaagcc tgggtcgtgc atggaaccga cggcctggat gagatgacga cgaccggcgt
     1441 gacgcgcgtc gcgatcctgg atcatggcca ggtgaccctt cgcgagatcg cgcctgaaga
     1501 tgcgggcctg aagcgcggtc cccttaccgc actcaaaggc ggcagcgcag aggacaacgc
     1561 gcgtgccatc cgcgatctgt tcgcgggctc caggaatgcg tttcgcgaca tcgtgctgct
     1621 gaacgcgggc gcggccctgg tcgtcgccga caaagccaag accatcggcg agggtgtcgc
     1681 agccgccgcc gaagcgatcg acaagggcca tgcggcaagc accctgacac aatcgcggat
     1741 cagcgagcgc gccggggtat gaactgatga gcatcctgga acgcatcgcg gcctataaac
     1801 gcgaggaagt cgcggcagca aagaaagagc ggcccctgaa ggcgttgatg gcggacgcca
     1861 atgaggcgcc agctccgcgc ggcttccgcg ccgcgctcga agcggccaaa gcggcaaggc
     1921 atccgggcct gatcgcggag atcaaaaagg cgagtcccag caagggcgtg atccgggccg
     1981 atttcgatcc ggaagtgatc gcgcgcgatt acgagcgtgg cggcgccacc tgtctttccg
     2041 tgctcaccga tacgccgtcc tttcagggcg cggacgacga tctgatgatg gcgcgacagg
     2101 cgacgcatct gccggtgctg cgcaaggatt tcatgctgga accttatcag gtggtgcaaa
     2161 gccgcgcact cggcgccgac tgcatcctcg tcatccttgc catgctgagc gatgcgatgg
     2221 cgacgagatt gatcgagacg gccgaggaat ggggcatgga cgccttggtc gaggtccatg
     2281 acgaaaacga ccttaaatgc gcgctggcgc tcgatgccga attgatcggg atcaacaatc
     2341 gcaatctggc gacattcgtg actgacatcg gcacggcgat acggctgaaa ccgctgatac
     2401 cggcggaccg gcatgtgatt gccgaaagcg gcctctccac gccgggggat ctgacgcggc
     2461 ttgccgcggc cggcatcacg tcctatttga tcggcgaaag tctggtgcgc gcggacgatg
     2521 tcgaagccgc gacgcacgca ctgctttcag gcgtgaaact atgagcaagc tttctcatgt
     2581 cgatgaatcc ggacgcgcgt ccatggtcga tgtgtccggc aaggacgcga caccccgttt
     2641 cgcgaccgcc gaagcggtcg tcgtgatgcc tgaggaagca ttcgctctcg ttgtagccgg
     2701 cgagatcaag aagggcgatg tcctggcgac cgcacgcatc gcagggatca tggccgcaaa
     2761 gaagacatcc gagttgatcc cgctctgcca tccattggcg ctcacccata ttggggtgga
     2821 gttcacgcct ttgcccgagc ggcacgcgtt ccgcatcgtc gctaccgcca agaccgccgg
     2881 ccagaccggt gtggaaatgg aggcgatgac ggcagcgagc atggccgcgc ttacggtcta
     2941 tgacatggtc aaggccgtgg acaaggggac ggtgatcgaa accgtccgcc tactgacgaa
     3001 atcgggtggt aagagcggcg actatgaagc gccctccccg ccggatgcgc acgagccgca
     3061 aaccaagcct gcacggttcg cgaaggcggc cccgcgcgtg ctgatgcacg aagccgcaag
     3121 tccccgcgca gcccacaccg gaggggatcg tgaagccctg cggcgcttca ttcaggcacg
     3181 gcgcttgcgt gcgagcgaat gggcgaaatc cgccgccgtc cccgtgaacg agctttacgg
     3241 ttatctctcc ggccattcgc gtgccctctc ctcggattcg gaggaaaagc tcgccagagc
     3301 cgcgcgcgca agcctcgatg agatgttcgg ccgcaagggg acgcgatgat ctccgtcaat
     3361 gaggcggtcg cgcggatcgc ccgcggtctg acacggctgt cttcggaatc cgtaatggtg
     3421 gccgaggccg ccggacgggt gctcgccgag gatgcggtag cgcgcctgga tcatccaccg
     3481 acgccgatgt ccgcgatgga cggctatgcg gtgcgccgcg ccgatgcccg cgccggcgcc
     3541 gaattttcca tcatcggcga agctccggcc gggcggccgt ttccgggacg tatcggcgag
     3601 ggtcaggcgg tgcgcatctt cactggcggc gtggtgccgg aaggtgcgga cgcggtggtg
     3661 atccaggagg atacagtccc cctcgcgtcc aatgacggcg tggtcttgaa agtgcgcatt
     3721 acggaaaccc cgtaccccga cgaaaatgtc cggccgcgcg ggctggattt ccgcaagggc
     3781 gaaatcttgc tgccgcgagg ccatcgcatt tccccgcgcg acgtcgcgct catcgcggcg
     3841 ggcgatcttg caaaggtctc ggtcatccgt aaaccgtgcg tagcactggt cgcgaccggc
     3901 gacgaacttt cgcgtccggg agaacctcgc aaggatggcg gcattgtcgc ctcatcgatt
     3961 tacgcgctgc aggcgatggt gcgccaatgg ggcggcgagg ccatcgatgc cggcatcctg
     4021 ccggaccggc ccgaggcttt cgcagctttg cccgaggcca cgaaaaacgc cgatcttgtc
     4081 gtcacgcaag gcggcgcatc ggtcggcgat catgacctcg ttcaaagtgc gctgaagccg
     4141 ttcggtttca cgctcgattt ctggcggatc gcgatgcgtc ccggcaagcc gctgatcttc
     4201 ggccgcttgc gcgatacgcc cttcatcgga ctcccgggca atccggtttc ggcgatggtg
     4261 tgcgcggtgc ttttcatcca acccgccatc gccgctatgc tcggaatgac ctatgagccg
     4321 cccatcgtga aagcgcggct cggttcggct ttgcgcgcaa acgggcgtcg tcaggattat
     4381 atccgcacgc ggctcgtgcg gcacgggggg attctcaccg ccgaaccttt cgcgctg
//
LOCUS       DASYEG010000231         3405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4286110, whole genome shotgun sequence.
ACCESSION   DASYEG010000231 DASYEG010000000
VERSION     DASYEG010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4286110"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="VGM72_10110"
     CDS             complement(<1..1119)
                     /locus_tag="VGM72_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265662.1"
                     /translation="MTAATISDVRTVADAILYEGYILYPYRKSSLKNQQRWTFGGLFP
                     RSYALRGGGDRWTMTCECLLRGDAQTRLRLQLRFLQVAARRIARLAAPVRTLDAGALP
                     PHAVVEGMDINGRSYTPWDEAVPREEVVLAGRLEELAANPLIRDLFYSAGTGVEPIAA
                     EDGLVQGLILRQTESVAVRLTLSAEPVAVDAFRLRAVVENITALDVPAGLKRDAAAPF
                     ATASNHLILSVEGGAFASLTDPPADLAEAAKACANDGCWPVLAGADGMTDTILAAPII
                     LYDYPRIAAESQSDLFDGTEIDEILILRILTMTDAEKREMASGDARASDLLAKLESLT
                     PDDLGKLHGTWRDSSRQKPELVSIAHGGLSVGTRVRLKP"
     gene            complement(1116..1751)
                     /locus_tag="VGM72_10115"
     CDS             complement(1116..1751)
                     /locus_tag="VGM72_10115"
                     /inference="COORDINATES: protein motif:HMM:NF039986.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6084 family protein"
                     /protein_id="HEY4265663.1"
                     /translation="MPELAFALDGVEIERYAVTPTLRFRLRITADQPVENIALQCQLR
                     IEPMRRAYGGDEHERLADLFGEKARWGDTLKSFLWTIVPVNVPRFDDVTIVDAMVPCS
                     FDFDVAATKYFHGLEGGEVPVSLLFSGSVFYRDPQDRLQISQLSWSQGLSHRLPAALW
                     QTLMDQYYPNAAWLRLDRETFERLYALKRRMGVGSFDAALSQLLSAPQVLQ"
     gene            complement(1744..2352)
                     /locus_tag="VGM72_10120"
     CDS             complement(1744..2352)
                     /locus_tag="VGM72_10120"
                     /inference="COORDINATES: protein motif:HMM:NF040271.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5947 family protein"
                     /protein_id="HEY4265664.1"
                     /translation="MHVGLQTTDGMRAVFALRRFVYPPAERCELCRARLASQHSHLLE
                     TRVGQVRCACGACSERYGTPEEGFLPVNSRKDKLTDFALSDGQWDAFQIPIDLAFLVR
                     RSDGLVALYPGPAGIIQAETNREAWTDLTEANPLLRDLAPEVEALAINRVHGRRDAWR
                     LSIDHCFSLAGTLRKEWHGLSGGPKVWEEIEHYFDRDEALHA"
     gene            complement(2357..2872)
                     /locus_tag="VGM72_10125"
     CDS             complement(2357..2872)
                     /locus_tag="VGM72_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265665.1"
                     /translation="MTARTQVEDTLRRIEELVQGLDRVKDPAARQAAQQLFAAVLDLH
                     GLALAKITARLVADPQGRDLYQDMGADEQIRAVLLLYGLHPDSAEERIRAAIAPLEAR
                     FNVRLHVSSVSEGIARVTMDPGVGDFDSLCPEIGRVLLDAAPDLDDIAIDREVAAGVT
                     MTAPPRAAAAG"
     gene            complement(2869..>3405)
                     /locus_tag="VGM72_10130"
     CDS             complement(2869..>3405)
                     /locus_tag="VGM72_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEY4265666.1"
                     /translation="KPAMDLEWQVPQWSNTIERNRARTYFQAYCASMAMFFLEKAFAE
                     VQGGRTKTWTEFKVPDEAIGCGFHEAVRGVLSHHMVIRDGKIANYHPYPPTPWNATPR
                     DKFGTPGPYEDAVQGCPIFEENGPENFKGIDIMRTVRSFDPCLPCGVHMYVGNGKTLK
                     TLHQPMFGAAAHSHGNQK"
BASE COUNT          682 a         1063 c         1019 g          641 t
ORIGIN      
        1 gggcttcagc cgcacccgtg tcccgacgct gagtccgccg tgggcgatac tgaccagctc
       61 tggcttttgc cggctgctat cacgccaggt gccgtgcagt ttgcccaaat cgtcgggcgt
      121 gaggctttcc aacttcgcca gcaagtcgct ggcgcgcgcg tcgcccgacg ccatctcgcg
      181 cttttccgca tcggtcatgg tcaggatgcg caggatcaga atttcatcga tctcggtgcc
      241 gtcgaacagg tcggactggc tctctgccgc gatgcgcggg taatcgtaaa ggataatcgg
      301 cgcggccaga atcgtgtcgg tcatcccgtc cgctccggcc aggaccggcc aacaaccgtc
      361 attggcgcac gccttggcgg cttcggctaa gtcagccggg gggtcggtaa gggaagcaaa
      421 ggcgccgcct tcgacgctta ggatcaggtg gttggacgcc gtcgcaaacg gtgccgcggc
      481 atcacgtttt aggccggcgg gcacatcgag cgccgtgatg ttctcgacca cggcgcgtag
      541 gcgaaaagca tccaccgcta cgggttcggc cgataaggtc agccgcacgg caacgctctc
      601 ggtctggcga aggatcaagc cttgcaccaa cccgtcctca gcggcgatcg gttcgacacc
      661 cgtcccagca gaatagaaga ggtcccggat caaagggttc gcggccaatt cctccagcct
      721 accagcgaga acaacctctt cccggggaac cgcttcgtcc caaggcgtat aagaacgccc
      781 attaatgtcc atcccctcga ctaccgcgtg ggggggcaga gctcctgcgt cgagcgtgcg
      841 taccggcgca gccaaccgcg cgatacgccg tgccgccact tggagaaagc gcaactgaag
      901 gcgcaggcgg gtctgcgcat cgccgcgcag aaggcattcg caggtcatag tccagcggtc
      961 gccgccaccg cgcagcgcgt aactgcgggg gaacaggccg ccaaaggtcc agcgctgctg
     1021 gttcttcaag gaagacttgc ggtatgggta aaggatgtac ccttcataaa ggattgcatc
     1081 cgctaccgtg cgtacgtcgg agattgttgc cgcagtcatt gcagtacctg cggggccgaa
     1141 agaagttgcg agagggcggc gtcgaagctg ccgacaccca tccgccgctt caacgcataa
     1201 agccgctcga atgtctcgcg gtccagacga agccaggccg cgttgggata atattggtcc
     1261 atcagcgtct gccagagcgc agcggggagg cgatgactaa gcccctgaga ccaggaaagc
     1321 tgagaaatct gcagcctgtc ctgtggatca cgatagaaga ccgaaccgct gaacaggagc
     1381 gacactggca cttcaccgcc ctccaggccg tggaaatatt tggtagctgc gacatcgaaa
     1441 tcgaaactgc aaggcaccat ggcatcgaca atcgtgacat cgtcaaagcg cggcacattt
     1501 acgggcacaa tcgtccacaa aaagcttttc agggtgtcgc cccaacgtgc tttctcgccg
     1561 aataaatcgg ccagcctttc gtgttcgtca ccgccatacg ctcgccgcat cggttcgatg
     1621 cgcaactgac attgcaaggc gatattttct accggctggt cggctgtgat ccgtaaccga
     1681 aagcgcaggg ttggggtaac cgcgtagcgc tcaatctcca caccgtctag ggcaaaggct
     1741 agttcaggca tgaagggcct cgtcccggtc gaaatagtgt tcgatttctt cccaaacctt
     1801 gggaccgcct gacagcccat gccactcctt gcgcagcgtt ccggccagcg aaaaacaatg
     1861 gtctatggaa agcctccacg catcacgccg tccgtgcacc cggttgatgg cgagcgcttc
     1921 cacttccggc gccaaatcgc gaagaagcgg gttcgcctcc gtcaaatcgg tccacgcctc
     1981 cctattggtt tcagcctgga taataccggc agggccagga tagagcgcga ctaggccatc
     2041 gcttcttcgc accagaaacg cgagatcaat gggaatttgg aaggcatccc attggccatc
     2101 actcagagcg aaatccgtca gcttgtcctt gcgggaattg accggcagaa agccttcctc
     2161 cggcgtgcca tagcgctcgc tgcaagcgcc gcaggcgcag cgcacttggc caacccgtgt
     2221 ttccaacaga tggctgtgct gggaggctag cctggcgcgg cagagctcgc agcgttcggc
     2281 cggcggatag acgaaacggc gcagtgcgaa gaccgcccgc atgccatccg tggtttggag
     2341 gcccacatgc atgaggctat cccgcagccg cagcgcgggg cggcgccgtc attgtcacgc
     2401 ccgccgcaac ctcgcgatcg atggcgatgt catccagatc cggtgcggca tccagcaaaa
     2461 cacggcctat ctctgggcac agactgtcga agtctcctac gccaggatcc atcgtgactc
     2521 gtgcgattcc ctcagacacc gacgaaacgt ggagacgcac attaaaccgc gcttccagcg
     2581 gcgcaatcgc tgcgcggatg cgctcttccg cactgtccgg gtgtagaccg tacaacagca
     2641 gaacggccct gatttgctcg tctgcgccca tatcctggta aaggtcgcgg ccctgtggat
     2701 cggctaccaa gcgcgcggtg atcttcgcca gcgccaaacc atgcagatcc agcaccgccg
     2761 caaacaactg ctgcgccgcc tgccgggccg ccgggtcctt gacccggtcc agcccctgaa
     2821 cgagctcttc gatgcggcgc agtgtatctt ctacttgggt gcgcgcggtc acttttgatt
     2881 cccatggcta tgcgcggccg ccccgaacat gggctggtgt aaggtcttta gggtcttgcc
     2941 gttaccgaca tacatatgca cgccgcatgg caggcagggg tcgaagctgc gcacggtgcg
     3001 catgatatcg atgcctttga agttctccgg cccattttcc tcgaagatcg ggcagccctg
     3061 gacggcatct tcataaggcc cgggcgtacc gaacttgtcg cgcggcgtcg cgttccaagg
     3121 cgttggtgga tagggatggt aattggcgat cttgccgtcg cggatcacca tatgatggga
     3181 gaggacgccg cgcaccgctt cgtggaagcc gcaaccgatc gcctcgtctg ggaccttgaa
     3241 ctcggtccag gtcttggtgc ggccaccctg gacttcggcg aaggcctttt ccaggaagaa
     3301 catcgccata gaagcgcaat aggcctgaaa gtaagtgcga gcgcgattgc gctcgattgt
     3361 gttcgaccat tgtggcacct gccattccag gtccatggcg ggttt
//
LOCUS       DASYEG010000232         8406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4286606, whole genome shotgun sequence.
ACCESSION   DASYEG010000232 DASYEG010000000
VERSION     DASYEG010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 8406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8406
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4286606"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VGM72_10135"
     CDS             complement(<1..491)
                     /locus_tag="VGM72_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4265667.1"
                     /translation="MRETDSLLKGRIVLVTGASRGIGRAVAVALGRAGAHVVAVARTV
                     GGLEELDDDIKKEGGTATLVPLDLKDFAGIDRMAAAIHERWGRLDALFGNAGILGALT
                     PLSHLSPKTWDDLMAINVTANWRLIRDFDPLLRQSDAGRVLMMSSAVARLNRPYWGPY
                     AIT"
     gene            complement(506..1945)
                     /gene="purF"
                     /locus_tag="VGM72_10140"
     CDS             complement(506..1945)
                     /gene="purF"
                     /locus_tag="VGM72_10140"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY4265668.1"
                     /translation="MTTPFDDKLHEECGVFGVFGHEGAGALTALGLHALQHRGQEAAG
                     IVTFDGTQFISERHVGLVGDTFGKHGHTANLCGTNAIGHVRYSTTGGSIGRNVQPMFA
                     DLAGGGLAIAHNGNLTNARVLRRDLVRGGAIFQSTSDTEAIIHLAARSHYEFVVEKLI
                     DALKRVEGAYSLVALTSKKLIGVRDAYGVRPLVLGRLDDAWILASETCALDIIGAEFV
                     RDIEPGEMVVITAEGIESLHPFPPKQRRFCVFEYVYFARPDSMVEGRNVYEVRKRIGM
                     ELAHESPADADIIVPVPDSGVPAALGFSQASGIPFDLGIIRNHYVGRTFIEPTDRIRH
                     LGVKLKHNANREELKGKRIVLVDDSIVRGTTSKKIVEMVRDAGATEVHFRIASPPTTH
                     SCFYGVDTPNKEDLLAHNLDLEGMRDFIKADSLAFVSINGLYRAVGEARRNVAAPQFC
                     DACFSGDYPIEPLDSSESRRQLSLLVETV"
     gene            complement(1942..2538)
                     /locus_tag="VGM72_10145"
     CDS             complement(1942..2538)
                     /locus_tag="VGM72_10145"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEY4265669.1"
                     /translation="MSALSLTAIDFVVIAVVLLSAIFATARGLIHETFAILAWIAGGY
                     LAFRLTPFVQPLLHNMIAPPWLERFLVLLGIFILVFVPLAIFSRWLSAKVKGSVVSPV
                     DHVLGLVFGVCRGLVIVGVAYIAFAALVPLKNQPDILTKARLFPVIRNTSEMLRELVP
                     SGGTNLSSGNSASGYGARGRSTLDRLLHGKPENDSSSQ"
     gene            complement(2776..4149)
                     /gene="radA"
                     /locus_tag="VGM72_10150"
     CDS             complement(2776..4149)
                     /gene="radA"
                     /locus_tag="VGM72_10150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEY4265670.1"
                     /translation="MARAAPAFVCQSCGTAHPKWSGRCDDCGAWNTLVEEQAAPPAGA
                     SARRIGKGRAFALEGLSTVGEEPVRRLTRIAEFDRVAGGGLVPGSALLLGGDPGVGKS
                     TLVMQVLAAYAGTKKSAVYISGEEALAQVRLRAARMGLSDAPVQLGAATCVEDILATI
                     ATASPPDILVIDSIQTVWTSALEAAPGTIAQVRTATHDLVRYAKASGVAVILIGHVTK
                     DGQIAGPKAVEHLVDAVLYFEGERGHHFRILRAVKNRFGATDEIGVFEMTGTGLAEVP
                     NPSALFLGDRSGLAPGAAVFAGMEGTRPLLSEIQALTAPSGYGTPRRAVVGWDTGRLS
                     MILAVLDARAGLGLSGQDVYLNVAGGLKIAEPAADLAAAAALISSFTGRPLPKDCVVF
                     GEIALSGDVRPTPHAEARLREAAKLGFGRALVPAGTKAGAAGLALEPIADVADLVHLV
                     GGEQRPR"
     gene            complement(4150..4923)
                     /locus_tag="VGM72_10155"
     CDS             complement(4150..4923)
                     /locus_tag="VGM72_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4265671.1"
                     /translation="MNPVPKIELKGVKKRFGPKIVLDGIDLTINAGDSLVVIGGSGSG
                     KSVTIKCVLGIIRPDVGSIRVDGEEVTRLRGAKRDAYLRQFGMLFQGGALFDSLPVWE
                     NVAFGLIQGRGIDRRRARDIAVEKLAKVGLGPEVADLSPAELSGGMQKRVGLARAIAA
                     DPAIIFFDEPTTGLDPIMADVINDLIATTVRDLKVTALSITHDMASARKISNRIAMLY
                     KGKIIWDGATQDIDNSGNPYVDQFIHGSAKGPIQMEVRR"
     gene            complement(4934..5716)
                     /locus_tag="VGM72_10160"
     CDS             complement(4934..5716)
                     /locus_tag="VGM72_10160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4265672.1"
                     /translation="MNKFNVFAAIGRVFLAFLGATGRLAVFTGEALSHCVRPPIFWGL
                     IGQQMLRIGYFSLPVVGLTAFFTGAVLCLQIYVGGNRYGAESFVPQIVVLGITRELGP
                     VIAGLMVAGRVAAAIAAEIGTMRVTEQIDALTTLSTNPVKYLVTPRLIAAVISMPILV
                     AIADAIGVYGGYIVATDSLNFNGAVYLKNTANFVTQEDVVSGLIKAAVFGFIIALMGC
                     YNGFHSKGGAQGVGAATTDAVVSASILILAANYIMTSVFFTH"
     gene            complement(5709..6836)
                     /gene="alr"
                     /locus_tag="VGM72_10165"
     CDS             complement(5709..6836)
                     /gene="alr"
                     /locus_tag="VGM72_10165"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY4265673.1"
                     /translation="MNKPAPESAYASDHAAAHLTVRLGAIAANYREVLRRAASAAAAP
                     VVKANAYGMGIGPVAHTLLNAGADTFFVARLEEGIEARTLIPSARIFVLDGVAPGAAP
                     ALVTNRLTPVLNSLDEIAEWSARAKSERRELDAALHIDTGMNRSGLSHGELSQLAARI
                     REALVGINLVLVMSHLARADEPDAEMNWTQLARFRAGLAALPPAPASLAASAGIELGR
                     DFHFDLVRPGLAIYGGNPMPSRPNPFRTVASLTGQVLQIRHIDAGETVGYAATFTAMR
                     PSALATVASGYADGLIRARSLKGHAAIDGIRVPFAGRMSMDLLALDVTDVPEAARARG
                     AVVEFMGDVVTLEEVAEAAGTITHEVLTAISPRARRIYVDE"
     gene            6982..8193
                     /locus_tag="VGM72_10170"
     CDS             6982..8193
                     /locus_tag="VGM72_10170"
                     /inference="COORDINATES: protein motif:HMM:NF005761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain"
                     /protein_id="HEY4265674.1"
                     /translation="MADKSVLISGIGIAGPTLAFWLHRAGFKPTLIERSPRLRTGGYV
                     IDFWGLGYEIAERMGLIEDIHRAGYHMRELRIVDDRGERVAGFGPKVFGELTGGRYVT
                     LGRSDLSRLLYEKAKDMTEVIFGDEIAGLQEQADGMRVRFKHGAERRFDLVMGADGLH
                     SDVRRLAFGSDDRFEKQLGYAVAAFEVQGYRPYDEEIYVMYGQPGLMLGRFAMHDDRT
                     LFLFVFTTGSDSLPATLEAQKAMLRQRYRGGKWECSRILSELDRTRELYIDAVSQIRM
                     ESWSRGRVALTGDAAFCVSLLAGQGSALAMISAYVLAGELASGDGGYAQAFVRYQAIL
                     QDYIGTKQRGAERLAPVFAPRTQWGLNFRNLVIKTFAIPGLAKFVAGRDIIDTLRLPD
                     YRWPVLENEAS"
BASE COUNT         1626 a         2652 c         2629 g         1499 t
ORIGIN      
        1 ttggtgatgg catagggccc ccaatagggg cggttcaacc gcgccaccgc cgaactcatc
       61 atcagcaccc gccccgcatc ggattggcgc agcagcggat cgaaatcccg gatcaggcgc
      121 caattggcgg taacgttgat cgccatgaga tcgtcccacg tcttcgggct cagatgcgag
      181 agcggcgtca gcgcgcccag gataccggca ttcccgaaca gcgcatcgag tcgtccccaa
      241 cgttcatgga tcgcggccgc catgcggtcg ataccggcaa aatccttcag atcgagcggc
      301 accagggttg cggtgccgcc ttctttcttg atgtcgtcgt cgagttcttc cagccccccc
      361 acggtgcggg caaccgcgac gacatgggca ccggctctgc ccagcgccac cgcgaccgcg
      421 cgcccgattc cgcgcgaggc gccggtcaca agtacaatgc ggcctttcaa gagggagtcg
      481 gtttcacgca tggatgccgt tcgcgctaaa ccgtttcgac cagcagcgag agctggcgac
      541 gggattcgct ggagtcgagc ggttctatcg gataatcgcc cgaaaagcag gcgtcgcaga
      601 attggggcgc ggcgacattg cgtcttgctt cgcccaccgc acgatacagc ccattgatcg
      661 agacgaatgc cagactgtcc gccttgatga agtcgcgcat cccttcgaga tcgagattgt
      721 gcgcgagcaa atcttccttg ttcggcgtat cgacgccata gaagcaggaa tgcgtggtgg
      781 gcggactggc gatacggaaa tgcacctcgg tggcgcccgc atcgcgcacc atctcgacga
      841 tcttcttcga tgtcgtgcca cggacgatgg aatcgtcgac cagcacgatg cgcttgccct
      901 tcagttcctc gcgattggcg ttgtgcttca gcttgacgcc cagatggcgg atacgatcgg
      961 tcggctcgat aaaggtgcgt ccgacataat gattgcggat gattccgaga tcgaagggaa
     1021 tgccggaggc ctgcgaaaag ccgagcgccg ccggcactcc ggaatccggc accggcacaa
     1081 ttatgtcggc atctgcgggg gattcgtgcg ccagctccat cccgatgcgt tttctcacct
     1141 cgtagacatt acggccttcg accatggaat ccggccgggc gaaatagaca tattcgaaga
     1201 cgcagaatcg ccgctgcttc ggcgggaagg gatgcaggga ttcgatccct tccgcggtga
     1261 tgacgaccat ctcgccgggc tcgatatcgc gcacgaactc cgctccgatg atatcgagcg
     1321 cacaagtctc cgatgccaat atccaggcat cgtcgagacg gcccagcacc agcggccgca
     1381 cgccataggc atcgcgcacg ccgatcagct ttttcgatgt gagcgccacc agcgaatagg
     1441 cgccctcgac acgtttgagt gcatcgatca gcttttcgac cacgaattcg taatggctgc
     1501 gcgcggccag atggatgatc gcttcggtgt ccgacgtcga ttggaagatc gcgcccccgc
     1561 gcaccagatc gcgccgcagt acccgcgcat tggtgagatt gccattatgg gcgatcgcaa
     1621 ggcccccgcc ggccagatcg gcaaacatcg gttgcacatt gcggccgatg gagccccctg
     1681 tcgtggaata gcgcacatgc ccgatggcgt tcgtcccaca caaattcgcg gtatggccat
     1741 gcttgccgaa agtgtcgcca acaaggccga catggcgttc cgagatgaac tgcgtgccgt
     1801 caaaggtgac gatgcccgcc gcttcctggc cgcgatgctg gagcgcgtgc aggccgagcg
     1861 cggtcagcgc gccggcgcct tcatgaccga aaacgccgaa aacaccgcat tcctcgtgca
     1921 atttgtcgtc gaacggtgtg gtcattgcga ggagctgtcg ttctctggct tgccgtggag
     1981 cagtctgtct agcgtgctgc gtccacgcgc gccatagccc gaggcgctgt ttccgcttga
     2041 gaggttcgtg ccgcccgatg gcacaagctc gcgcaacatt tcactggtgt tgcggatcac
     2101 gggaaacagc cgcgccttgg tcaatatgtc gggttggttc ttcagcggca ccagcgccgc
     2161 gaacgcgata taggcaaccc cgacgatcac caatccgcgg cagacgccga acaccaggcc
     2221 cagcacgtga tcaacggggc tgaccaccga acccttcacc ttggccgaga gccagcggct
     2281 gaaaatcgcg agcggaacga ataccaggat gaagatgccc aaaaggacga gaaacctctc
     2341 cagccagggc ggcgcaatca tgttgtgcag aagcggctgg acaaaaggcg taaggcggaa
     2401 cgccagatag ccgcccgcga tccatgcgag gatggcaaaa gtctcatgga tcaatccgcg
     2461 ggcggtcgcg aaaatcgccg acagaagcac cacggcgata accacgaaat cgatggccgt
     2521 cagcgatagt gcgctcatga ttcgcaagcc tctccggcgg agattaccac ttttgcgcag
     2581 gtctcaggct cggccacccc ctccccccaa ctttttgccg tcctggcggg attttttcga
     2641 gggatttcct gccgatttac ggtcattgcg acagaaaatt ccggtgcaat ttcggtgcaa
     2701 ttgggtgcat ttgcgcatca tgcggacctt ggccggtcat cggctcctcc aacatggggt
     2761 cttccggcat cgttttcatc gggggcgctg ttcgccccct accaaatgga ccaggtcggc
     2821 cacgtccgcg atcggctcca gcgcgaggcc tgccgcgccc gctttggtcc ccgccggcac
     2881 cagtgcgcgg ccaaacccaa gcttcgccgc ctctctcagc cgggcttccg catgcggcgt
     2941 gggacgcaca tcgcccgaaa gcgcgatctc gccgaacacc acgcaatcct tcggcaaagg
     3001 ccgcccggtg aaggaggaaa tcaacgccgc cgccgccgcc agatcggccg cgggctccgc
     3061 gatcttcaaa ccgccagcca cattcaaata gacatcctgc cccgagagtc cgaggccggc
     3121 gcgggcatcc agcacggcga gaatcatcga aagccgcccg gtatcccagc ccaccaccgc
     3181 gcggcgtggc gtgccgtaac cggagggcgc ggtgagcgcc tggatttccg acagcagcgg
     3241 acgcgtgcct tccatgccgg cgaacacggc ggcccccggc gccagtcccg aacggtcgcc
     3301 caggaacaaa gccgatggat tgggcacttc ggccagaccc gttccggtca tctcgaacac
     3361 gccgatttca tcggtcgcgc cgaaacgatt tttcaccgcg cgcaggatgc ggaaatgatg
     3421 tccacgctcg ccttcgaaat agagcaccgc gtcgaccaga tgctcgaccg ctttcgggcc
     3481 tgcgatctgg ccgtccttgg tgacgtgccc gatcaggatg accgccacgc cgctcgcctt
     3541 ggcatagcgt acgagatcat gcgtggcggt gcgcacctgc gcgattgttc cgggagccgc
     3601 ttcgagcgcc gacgtccaga cggtctggat ggaatcgatc accaggatgt ccggcggcga
     3661 agcggtggca atcgtcgcca ggatatcctc gacgcaggtg gcagcgccga gctgcacggg
     3721 cgcgtccgag agacccatgc gcgcggcgcg cagacgcacc tgcgcgagcg cctcttcgcc
     3781 cgagatatag acggcggatt ttttcgtccc cgcataggcg gcgagaacct gcatcaccag
     3841 cgtggacttt ccgacgccag gatcgccgcc caagagcagc gcggagcccg gcaccaggcc
     3901 gccgccagcc acgcgatcga attcggcgat tcgggtgagg cggcgcaccg gttcttcgcc
     3961 gaccgtggaa agcccttcga gcgcgaaggc gcggcccttg ccgatccgcc gcgccgatgc
     4021 gcccgcgggc ggggccgcct gttcttccac cagcgtgttc catgcgccgc aatcatcgca
     4081 gcggccggac catttggggt gcgccgtgcc gcaggattgg cagacgaagg caggagcggc
     4141 gcgcgccatc tagcggcgga cttccatctg gatcggacct ttcgccgaac catggataaa
     4201 ttgatccacg taaggattgc cgctgttgtc gatatcctgg gtcgcaccgt cccagatgat
     4261 cttgcccttg tagagcatgg cgatgcggtt cgagatcttg cgcgcgctcg ccatgtcatg
     4321 ggtgatggac agcgcggtca ctttgagatc gcgcacggtc gttgcgatca gatcgttgat
     4381 gacatcggcc atgatgggat caaggcccgt ggtcggctcg tcgaagaaga tgatcgccgg
     4441 atcggccgcg atggcgcgcg cgagcccgac gcgcttttgc atgccccccg acagttccgc
     4501 cggcgacaga tcggcgactt cggggccgag gccgaccttc gccagtttct cgacggcgat
     4561 gtcacgggcg cgcctgcgat cgattccacg gccctggatc aggccgaagg cgacgttctc
     4621 ccacaccggc aggctgtcga agagcgcgcc gccctgaaac agcatgccga attgtcgcaa
     4681 ataggcatcg cgcttggcgc cgcgcagacg cgtgacttcc tcaccatcga cccggatcga
     4741 gcccacatcg ggacggatga tgccgagcac gcatttgatg gtgaccgatt tgccgctgcc
     4801 cgatccgccg atcacgacca gcgaatcgcc ggcattgatg gtgagatcga tcccgtcgag
     4861 cacgatcttt ggaccgaagc gcttcttcac gcctttgagt tcgatttttg ggacggggtt
     4921 catcgcaaaa gactcaatgg gtgaagaaga cggacgtcat gatgtaattc gcggcgagga
     4981 tcaggatgga cgccgacacc acggcatcgg tggtcgcggc gccgacgcct tgcgcgccgc
     5041 ccttggaatg aaagccgttg taacagccca tcagcgcgat gatgaagccg aacaccgccg
     5101 ccttgatgag acccgacacc acgtcttcct gggtgacgaa attcgcggtg ttcttcagat
     5161 agaccgcgcc gttgaaattc aggctgtcgg tcgcgacgat atagccgcca tacacgccga
     5221 tcgcatcggc gatggccacc aatatgggca tgctgatgac ggccgcgatg agccggggcg
     5281 tgacgagata tttcaccgga ttggtggaaa gcgtggtcag cgcgtcgatc tgttcggtga
     5341 cgcgcatggt gccgatctcg gccgcgatgg cggccgccac gcgccccgcc accatcagcc
     5401 ccgcgatcac cggccccagc tcacgcgtga tgccgagcac gacgatctgc ggcacgaagg
     5461 attccgcgcc gtagcgattg ccgccgacat agatctgcag gcacagcacc gcccccgtga
     5521 aaaaggccgt aagccccacc acgggcagcg agaaatagcc gatgcgcagc atctgctgtc
     5581 cgatcaagcc ccagaagatc ggcggccgca cgcaatgcga cagcgcttca cccgtgaaga
     5641 ccgcaagccg gcccgtggcg ccgagaaagg cgaggaagac gcgcccgata gcggcaaaga
     5701 cattgaattt attcatccac atagatccgg cgggcgcgcg gcgaaatcgc ggtgagcacc
     5761 tcatgcgtga tggtgcccgc cgcttcggcc acctcctcca gcgtgacgac atcgcccatg
     5821 aattccacca cagctccccg ggcgcgcgcg gcttcgggca catccgtcac atccagcgca
     5881 agcaaatcca tcgacatccg tcccgcgaaa ggcacgcgta tcccgtcaat cgccgcgtga
     5941 cccttaaggc tccgggcgcg gatgaggccg tcggcatagc ccgaggccac cgtcgccagg
     6001 gcgctgggcc gcatcgccgt gaaggtggcg gcgtagccca ccgtttcgcc cgcgtcgatg
     6061 tggcgaatct gcagcacctg ccctgtaaga cttgcaaccg tacggaaagg attggggcgc
     6121 gagggcatgg gattgccgcc gtagatggcg aggccggggc gtaccaggtc aaaatggaaa
     6181 tcgcgtccga gttcgatccc cgccgatgcc gcaagacttg ccggagcggg cggcagggcg
     6241 gcaaggccgg cgcgaaaacg cgcaagctgc gtccagttca tttcggcgtc cggctcatcg
     6301 gcacgggcca gatgactcat caccagcacc agattgattc ccaccagcgc ttcgcgaatg
     6361 cgcgcggcca attgggaaag ctcgccatgc gaaagacccg accggttcat gccggtatcg
     6421 atgtgaaggg cggcatcgag ctcgcgccgt tcgctcttgg cgcgcgccga ccattcggcg
     6481 atttcatcca aggagttcag cacgggcgtc agccggttcg tcaccagagc gggcgcggca
     6541 cccggcgcca cgccgtccag cacgaagatg cgggcggatg ggatgagggt gcgcgcctcg
     6601 ataccctctt ccagccgggc gacgaaaaac gtatccgccc ccgcattcag aagcgtgtgc
     6661 gcaaccgggc cgatgcccat gccgtaggca ttggccttca ccacaggggc tgcggcggcc
     6721 gatgccgcac ggcgcaacac ctcgcggtaa ttggcggcga tcgcgcccag ccgcacggtc
     6781 agatgcgcgg cggcgtgatc ggacgcatac gccgattcgg gtgccggttt gttcatgccc
     6841 cgagagaacg acagccgccc cggctagtca aatcggggcc ctgttccggt ggaggttgcg
     6901 gggccgcgtt tgcgaaactc cacaatgtcc aatctcgttg tatcggcagt ttcgtctgca
     6961 ggagaggagc ggttcttccg aatggccgat aaatccgtac tgatttcggg aatagggatc
     7021 gccggaccaa ccctcgcctt ctggctgcac agggctgggt tcaaaccgac gttgatcgag
     7081 cggtcgccca ggctgcggac cgggggttat gtcatcgact tctgggggct tggttatgaa
     7141 atcgccgagc gcatgggatt gatcgaggac attcatcgcg ccggctatca catgcgcgag
     7201 ctcaggattg tcgatgatcg aggcgagcgc gtggcgggct tcgggcccaa ggtcttcgga
     7261 gagttgaccg ggggacgcta tgtcacgctc ggccgcagcg atctttcgcg attgctgtac
     7321 gagaaggcca aagacatgac agaggtcatt ttcggcgatg agattgccgg ccttcaggaa
     7381 caggccgatg gcatgcgggt ccggttcaag cacggagccg aacgccggtt cgatcttgtg
     7441 atgggcgccg atggattaca ttcggatgtc cgcagactcg cattcgggtc cgatgatcga
     7501 ttcgaaaaac agctcggcta tgccgtcgcg gccttcgagg tccaggggta tcgcccgtac
     7561 gatgaggaga tatacgtgat gtacgggcag ccgggcctga tgctcggccg ctttgccatg
     7621 catgacgacc gcacgctgtt cttgtttgtg tttacgaccg gcagcgattc gcttccggca
     7681 acgctcgagg cgcagaaggc gatgctgcgg caacgctatc gcgggggaaa gtgggaatgc
     7741 tcccgcatcc tgagcgaatt ggatcgcacg cgcgaacttt atatcgacgc cgtcagtcag
     7801 atcaggatgg aaagctggtc gcgcggccgc gttgccctca caggggatgc cgcgttctgc
     7861 gtgtctctgc tggccggcca gggctcggcc cttgccatga tttccgccta tgtgctggcg
     7921 ggtgaactcg ccagcggcga tggagggtac gctcaggcat tcgtaagata tcaagcgatt
     7981 ctgcaagact atatcggcac gaaacagcgc ggtgccgagc gtcttgcacc cgtctttgcg
     8041 ccaaggaccc aatggggttt gaacttccgc aatctggtga tcaagacatt cgcaattccg
     8101 ggcttggcga agtttgtggc gggccgggat atcatcgaca cattgcgcct tcccgactac
     8161 cgctggcccg tgctcgaaaa cgaggccagc tagtccgcgc caaaagccgg cccgggatgc
     8221 ccccccagtc ctgccagcag tgcctgcgag cggtgctggt gccgtgacta gggtctgtac
     8281 ccacatagat cgttgaaaat tttgacgaac agtgattcac cgtgatggga ggtggatcat
     8341 gcgatttgat ttatccgatg aggaatggac gctccttgaa ccgttgatgc cgaagagccg
     8401 caagag
//
LOCUS       DASYEG010000233         1786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2114411, whole genome shotgun sequence.
ACCESSION   DASYEG010000233 DASYEG010000000
VERSION     DASYEG010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2114411"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VGM72_10175"
     CDS             <1..415
                     /locus_tag="VGM72_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY4265675.1"
                     /translation="DPHNVGAILRTAAAFGVSAVVVQDRHAPPQSGTLAKAASGALEI
                     VPYVEVVNIARALDRLAEAGFWRVALAGDGDQPLAAATTGGDIALVVGSEGDGIRRLV
                     REHCDTVAVIAIDKAMESLNVSNAAAVALYEIRRG"
     gene            complement(573..1610)
                     /locus_tag="VGM72_10180"
     CDS             complement(573..1610)
                     /locus_tag="VGM72_10180"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY4265676.1"
                     /translation="MATFLALALATADRARLHDVMDKASAAAADRIRDDLLRPVLILA
                     RALDEKIFDPPKPHVVLALPAPDPNDMRTYAKAGVPPRAPVRLADVPAPQMAEPLFIA
                     PDLPQFQILEDDAADAASLEGGNLAETPERMQVRARLEQSLTRDLRENFDLFLFVGKG
                     ARGTAAQRFYVFRKEKDGRLALIYDWAASTGRERYEISPQGRRTFTATPSGFYQLDPK
                     RMYSQYQSRAWNGAMPYAMFFNWEQRGNLTGVAIHGTAGGGAAKLGKRASAGCIHISR
                     AHAKQLFELVRRDYGGQVPRFAYDEGSGTMSNRGALMRDAAGALTMTEGYRVLIDVED
                     VSGVHTMAQLY"
BASE COUNT          345 a          574 c          524 g          343 t
ORIGIN      
        1 cgacccccat aatgtcggcg ctattctcag aaccgccgcc gcgttcgggg tcagcgcagt
       61 cgtcgtgcag gaccggcacg ccccgcccca atcgggaact ctcgccaaag ccgcctccgg
      121 cgccttggaa atcgtgccct atgtcgaagt ggtgaacatc gcacgagcgc tggatcggct
      181 tgccgaagca ggtttctggc gggtcgcgct ggcgggcgat ggcgaccagc ccctcgccgc
      241 cgctaccact ggcggcgata tcgcgctggt ggtgggttcg gagggtgacg gtatccgccg
      301 tctggtgcgg gagcattgcg acactgtcgc cgttatcgcg atcgacaagg ccatggagag
      361 cttgaacgtc tccaacgccg ccgccgtcgc cttgtacgaa attcgccgcg gctaagacgg
      421 cgccccgcgc ggaaccatcc atccgcccgc cggttatcac cccgatatct tcgggagaat
      481 ttacatgcgc tcagttttgc tttgggcgat cggcattccc ctgcccgtca tcatcattct
      541 gtggctcgtt acgggacacg cctgatttcc cctcaataga gctgcgccat ggtgtgcacg
      601 cccgaaacat cctcgacatc gatcaatacg cgatagcctt cggtcatcgt cagcgcgccg
      661 gccgcgtctc gcatcaatgc gccccgattg ctcatcgtgc ctgatccttc atcataggcg
      721 aagcgcggca cctgcccccc ataatcgcgg cggaccagct cgaagagctg cttcgcgtgg
      781 gcacgcgaaa tatggatgca tcccgcactg gcgcgctttc ccaatttcgc agcaccacca
      841 ccagccgtgc catgaatggc gacgccggtg agattgccgc gctgctccca attgaagaac
      901 atcgcgtagg gcatcgcgcc attccaggcc cgcgattgat actgggaata catccgtttc
      961 ggatcgagct gataaaagcc actgggtgtg gcggtaaacg tccgtctgcc ctgcggcgag
     1021 atttcatatc gttcgcggcc cgttgacgcc gcccaatcat agatcagagc gaggcggcca
     1081 tctttttcct tccggaaaac atagaagcgc tgcgccgccg tgccacgcgc ccctttgccg
     1141 acgaacagaa acagatcgaa attttcgcgc aaatcccgcg tcaggctttg ctccagccgc
     1201 gcgcgtacct gcatacgctc cggtgtctcg gcaagattgc cgccttcgag ggaggccgcg
     1261 tctgccgcat cgtcttccag aatctgaaat tgcggcagat caggcgcgat gaacaaaggt
     1321 tcggccattt gcggcgccgg cacgtcggcc aaccgaaccg gcgcgcgcgg cggcaccccg
     1381 gctttggcat aagtgcgcat atcgtttgga tcaggcgccg gcaaagcaag aaccacatgc
     1441 ggcttcggcg gatcgaagat tttctcgtcc agtgcacgcg cgagtatcag aaccggacgc
     1501 aacagatcgt cacggatccg gtccgctgcg gccgcgctgg ccttgtccat cacatcgtgc
     1561 aggcgagccc gatccgccgt tgccagggcg agcgcaagaa aagtcgccat ggcgaagtag
     1621 caaattccca gaatacgcaa actgaacata accgccggaa gagtgggttc cggcttttat
     1681 agccaagcgg ggcaaaactg tctcgttttg agacggttcg gttaatgccg ccgggcggac
     1741 gtctgagacc ttgaagctag gaggccgaga atttcccgga tttggc
//
LOCUS       DASYEG010000234         8350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1390498, whole genome shotgun sequence.
ACCESSION   DASYEG010000234 DASYEG010000000
VERSION     DASYEG010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 8350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8350
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390498"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..557
                     /locus_tag="VGM72_10185"
     CDS             177..557
                     /locus_tag="VGM72_10185"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY4265677.1"
                     /translation="MDDKERFDGGMKIRREVLGAEYVDRGMAGADDFMMAMQDLTTRY
                     CWGDIWSRPGLDRKTRSMLNLAMISALNRPHELKVHVKGALTNGVTKDEIKEILLQVA
                     IYAGVPAGMDSFRIANEAFKEAEK"
     gene            complement(564..1031)
                     /locus_tag="VGM72_10190"
     CDS             complement(564..1031)
                     /locus_tag="VGM72_10190"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4265678.1"
                     /translation="MQKISTFLWFDKEAEEAANFYVSIFKNSKIIAISRYGDAGPGPK
                     GSVMVVNFQLDGVDFMALNGGPNFKFTEAISLMVKCETQAEIDMFWEKLSAGGKTNVC
                     GWLKDKYGLSWQVAPAQLGDLLGGDQARTNRVMAAVMTMTKLDLAEMQKAAAG"
     gene            complement(1045..2277)
                     /locus_tag="VGM72_10195"
     CDS             complement(1045..2277)
                     /locus_tag="VGM72_10195"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY4265679.1"
                     /translation="MAGSDAKDRPLSGVRVLDLTQFLSGPYATQMFADLGADIIKLEP
                     PQGDPIRVVPPHFVGEDSVYYLCINRNKRCVAVDMKMDAGRDLVKRLALASDIVMENF
                     RPGVLDRLGLSVDALRAQKPALIWCSISGFGQDGPYRNKPAYDMIVQALSGGMSLTGE
                     TGGAPVRAGIPIGDLAAGLYASVATLAALNRRHRTGLGDTIDISMLDCQAAMLCYQAA
                     YHMHSGIVPGRQGRGHESIPTYRSFEAKDGIHIVITANTERMWQGLTRALGHEEWLAD
                     PRFTTNRERLANKHALWPMLEEAFRARDADEWIPILEREEIPVGVVNTLDRVVADPQI
                     NHRNMVLELASRDGRAARVTGNPMKFKDAAPEEKNYPRALGADTAAVLKDVLDLSPEE
                     IAELVRGKAVIARNSTGD"
     gene            complement(2284..3423)
                     /locus_tag="VGM72_10200"
     CDS             complement(2284..3423)
                     /locus_tag="VGM72_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEY4265680.1"
                     /translation="MTGVSVIGVGMIPFGKYPEKNIADLGWPAVKAAILDANIDPKRI
                     QAVYSGTARGGAMVGQRIMGRIGLAGIPIVNVENACSSSSGALAQAVIAVSAGACDVA
                     LVIGVEKLTKFGGGTLPLDEDDWEVRLGLSMPALYAMRAQRYMHDYGVSAEKIAGISV
                     KNRANGTLNPDAQMRKDVTIAEVLASRMIADPFTLLQCCPTGDGAAAIILASGKMARQ
                     FRNDPIQVRASHLTSGKFMAGFRDMTSPEITVRGAAETFEQAGLGPEDIDVAEVHDAF
                     SIAELLYYEAFGFCPRGQGTALLESGATKIGGRIPVNPSGGLLAKGHPVGATGAAQVV
                     EIVRQLRGEAGPRQVEGAKVGLSHATGGGISGFDHGACSIHIFQR"
     gene            complement(3420..3830)
                     /locus_tag="VGM72_10205"
     CDS             complement(3420..3830)
                     /locus_tag="VGM72_10205"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEY4265681.1"
                     /translation="MTVSALEQPGSTLLCSDAVALAADGRAILIGGACSDCGNQTFPR
                     APVCCVCMSEAIQPKAMPCTGTLYAFSTVHVAAKKWKIPMCIGYVDLPNGPRVFTHLQ
                     GDLAIGDAVEVAMGIVGEDENGPIRSFVFKRSAA"
     gene            3956..6322
                     /locus_tag="VGM72_10210"
                     /pseudo
     CDS             3956..6322
                     /locus_tag="VGM72_10210"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
     gene            6373..7317
                     /locus_tag="VGM72_10215"
     CDS             6373..7317
                     /locus_tag="VGM72_10215"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4265682.1"
                     /translation="MRITRREFGALSGAVLAAGAFSAQADAGEAAAMPPGAVDCHNHV
                     MGASAKYPYAAERTYTPPEAPVAALKALRAEIGTARNVLVTPSVYGTDNRCMVDALAE
                     LAGTARGIAVLPLDVSDAELHRLNAAGVRGIRIALGGTGRYDSKTAPAALASFATRFA
                     PLGWNIEMVGNLTALMPMAPAMADLKVPVVLDHFAGANGGDGVKQDGFAALLELVRGG
                     NAYVKLSAPYNRSKRADFSDMLPLARALLETRPDRMLWGTNWPHPDQIPGRPISEITP
                     YHVVDNKNLVRLFAEWCPDAGMRKTILVDTPVRLYRFS"
     gene            7320..8174
                     /locus_tag="VGM72_10220"
     CDS             7320..8174
                     /locus_tag="VGM72_10220"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY4265683.1"
                     /translation="MMEVRKLGAAMGAEILGADLNALSSDDFAQIRDAFHTHQVIAIR
                     DQTLSPTAQIDFSRRFGALEDQLNAHYTVPDYPEVLVLSNDVKDGKPIGLIDGGDFWH
                     SDSSHRECPSMATILYAVKNPRQGGDTEFASMYAAYDSLAPDMKRRIAPLKGIHAVSK
                     LKNKRVTVSPRRPDGKDFYEKQKSLPDQSWPMVRTHPVTGRKALYVSPRFTIGIEGMD
                     TAEADEILDDLFAHQIRPEFVYRHHWQNRDLVMWDNRAVIHQATGGYVYPDVRTMYRT
                     VVAGDRPF"
BASE COUNT         1547 a         2626 c         2604 g         1573 t
ORIGIN      
        1 tacacgcggc ccaagacgct ggacgagatg atcgatcatt ccatcgggcg gatgctcgat
       61 ctcttcggca tcgacatggg cacggtgcat cgctggaagg aaccggcgat tcccgacccc
      121 agcggcatgc cgaagaagcg cggcaagacc accgaagtct gacaggaata gaaaaaatgg
      181 acgacaagga acgctttgac ggcggcatga agatccggcg cgaggtgctg ggggcggagt
      241 atgtcgaccg cggcatggcg ggggccgatg atttcatgat ggcgatgcag gatctcacca
      301 cgcgctattg ctggggcgat atctggtcgc gcccaggcct cgaccgcaag acgcgctcga
      361 tgctcaatct cgcgatgatt tcggcgttga accggccgca cgaattgaag gtgcatgtga
      421 aaggcgcgct gaccaacggc gtcaccaagg acgagatcaa ggaaatcctt ctgcaggtgg
      481 cgatctatgc cggcgtgccg gcgggcatgg actcgtttcg catcgcgaac gaagccttca
      541 aggaagccga gaagtaaccg gctttatccc gcggcggctt tttgcatttc ggcgaggtcg
      601 agcttggtca tcgtcatgac cgcggccatg acacgattgg tgcgcgcctg atcgccaccc
      661 agaagatcgc ccaattgcgc aggggccacc tgccatgaca ggccgtattt gtccttgagc
      721 cagccgcaga cattggtctt gccacccgcc gacagtttct cccagaacat gtcgatttcg
      781 gcttgggtct cgcatttgac catgagcgag atcgcttccg tgaatttgaa atttggcccg
      841 ccgttcagcg ccatgaaatc cacgccgtcg agctggaagt tcaccaccat caccgagccc
      901 ttcggccccg ggccggcatc gccataacgc gagatcgcaa tgatcttgga attcttgaag
      961 atcgagacat agaaattcgc ggcctcttcg gcctctttgt cgaaccacag gaatgtgctg
     1021 atcttctgca cgggttttct ccttttaatc gccagtggaa ttgcgggcga taacggcctt
     1081 gccgcggacc agttccgcga tttcttccgg tgacaaatcc aggacatcct tcagcacggc
     1141 tgccgtatcg gcgccgagcg cgcggggata gtttttctct tccggcgccg catccttgaa
     1201 tttcatcgga ttgccggtga cgcgcgcggc gcggccatcg cggcttgcca gttccagcac
     1261 catgttgcgg tgattgattt gcgggtccgc gaccacacgg tcgagcgtat tgaccacgcc
     1321 caccggtatt tcctcgcgtt ccagaatcgg aatccattca tcggcatcgc gcgcgcggaa
     1381 tgcctcttcc agcatcggcc agagcgcgtg tttgtttgcg aggcgttccc tgttcgtggt
     1441 gaaacgggga tcggcaagcc attcttcgtg gcccaacgcg cgcgtcaatc cctgccacat
     1501 ccgttcggta ttggcggtga tgacgatatg gatgccgtcc ttcgcttcga agctgcgata
     1561 ggtcggaatg gattcatgtc cgcgtccctg acggcccggc acgattcccg aatgcatgtg
     1621 ataggcggcc tgatagcaca gcattgcggc ctgacaatcg agcatggaaa tatcgatggt
     1681 gtcgccgagg cccgtgcgat ggcgccggtt cagcgcggca agggtggcga cgctcgcata
     1741 gagacccgcc gcgagatcgc cgatggggat gccggccctc accggtgcgc cgccggtctc
     1801 gccggtcaga ctcatgccac ccgaaagcgc ctgcacgatc atgtcatagg cgggtttgtt
     1861 gcggtatggc ccgtcctggc cgaagccgga aatcgagcac cagatcagcg cgggcttttg
     1921 cgcgcgcagc gcatcgaccg atagtcccag gcgatccagc acgccggggc ggaaattctc
     1981 cattacgatg tctgaggcga gcgccaggcg tttgaccagg tcgcggccgg catccatctt
     2041 catgtcgacg gcgacacagc gtttgttgcg attgatgcag agataataga cgctgtcctc
     2101 gcccacgaaa tgcggcggca ccacgcggat gggatcgcct tgcggcggct ccagcttgat
     2161 gatgtccgcg ccgagatcgg cgaacatctg tgtcgcataa ggcccggaga gaaactgcgt
     2221 caggtcgaga acgcgcactc ccgaaagcgg ccggtccttg gcgtcactcc cggccatgcc
     2281 tgtctaccgc tggaaaatat ggatcgagca ggcgccgtga tcgaagcccg aaatgccgcc
     2341 gcccgttgca tggctgagcc cgactttcgc tccctcgacc tgacgcggcc ccgcttcgcc
     2401 ccgcaattgg cgcacgatct cgacgacttg ggcagcgccc gtcgcgccca cgggatggcc
     2461 cttggcgagg agcccgccgg aaggattgac cggaatgcgg ccgccgattt tcgtggcacc
     2521 gctttccaaa agtgccgttc cctgcccacg cggacagaac ccgaacgcct cataatagag
     2581 aagttccgca atcgaaaacg cgtcatgcac ttcggcgacg tcgatatcct ctggccccag
     2641 ccccgcttgc tcgaaggtct ccgcggcacc gcgcaccgtg atctcagggc tcgtcatgtc
     2701 gcgaaatccc gccatgaatt tgccgctggt caaatgcgaa gcgcgcacct ggatgggatc
     2761 gttgcggaat tgccgcgcca ttttgccgga ggccagaata atcgccgccg cgccatctcc
     2821 ggtagggcag cattgcagca gcgtgaatgg atcggcgatc atgcgcgagg ccagaacctc
     2881 ggcaattgtc acgtcttttc gcatctgcgc atcgggattg agcgtgccgt tggcgcgatt
     2941 tttcactgaa atgccggcga ttttctccgc gctcacgccg taatcatgca tgtagcgttg
     3001 cgcgcgcatg gcatagagtg cgggcatgga aagtccgagc cggacctccc aatcgtcttc
     3061 gtccagaggc agcgtgccgc cgccgaattt ggtcagtttc tcgacaccga tgacgagcgc
     3121 cacatcacag gcacccgcgc tgaccgcgat caccgcttgc gccagtgcgc ccgaactgga
     3181 cgaacaggcg ttctccacat tgacgatggg aatgccggca agaccgatgc gtcccatgat
     3241 gcgctgaccc accatggcgc cgccgcgcgc ggtgccggaa tagaccgcct ggatgcgttt
     3301 gggatcgatg tttgcatcca gaatcgcggc tttgacggcc ggccaaccga gatcggcgat
     3361 gttcttctca ggatatttac cgaatgggat catgccgacc ccgatcacgg aaacgcccgt
     3421 catgccgcgc tccttttgaa tacgaaggac cggatgggcc cgttctcgtc ttcgccgacg
     3481 atgcccattg cgacctcgac cgcatcgccg atcgccagat cgccctggag atgcgtgaac
     3541 acgcgcgggc cattgggcag atcgacatag ccgatgcaca tcggtatttt ccacttcttg
     3601 gcggccacat gcacggtgct gaaggcgtag agcgtacctg tgcacggcat ggctttgggc
     3661 tggatcgcct cgctcatgca cacacagcag acaggtgcgc gcgggaaggt ctgattgccg
     3721 caatcgctgc atgcaccgcc gatcaggatc gcccggccgt cggcagccag cgcgacggca
     3781 tcgctgcaca gaagcgtgct gcccggttgt tccaatgcgc taaccgtcat gatgctcagc
     3841 ccaaacggct ttcttctttc ctgtacaacg gatttgcgtt gcccgtaaat gcggggaagg
     3901 ccgaaggcat agcgaggagg ggaggggcgt gagcggaaaa tccatcggcg cgcgcgtgtt
     3961 gcggaccgag gatgcgcggc tgcttaccgg gcgtggcgaa ttcgtcgatg atatccgtct
     4021 gcccggaatg ttgcacgtcg ccttcgtgcg ggcgagccat gcgcatgcgc gcatcataaa
     4081 cgtggacgcc acggcagcgc tgacaatgcc aggcgtgcat gcagtgttca ccgccgccga
     4141 catgaatcac gcccttcaga ccacgcggct gcccatgctg gtgcccaatc cctattctaa
     4201 actctcgcgc acgcaattcg cactggcacg cagcgaggtt tgctatgtgg gcgaggcaat
     4261 cgccatcgtg atcgccgata cgcgctatcg ggccgaagac gcagccgcga tggtggcggt
     4321 cgagtacgag tccttgcccg cgatcggcga ttgccgggag gcggtcaaag cagatgcccc
     4381 gcgcgcgcat ctggatgcaa ccgacaacaa agcggcggcc ttcaaaaccg cctatggcga
     4441 tgccgccgcc gccttcgccg gggccgccca tatttataag gaggcgctgt ggcatcatcg
     4501 cggctgcggc catgctctcg aatgccgcgg cgtgctcgcc gatgttgcac gcgacggcgc
     4561 gctgacgctg tggtcgtcca cccagacgcc gcatctcgaa aagcgcgtgc tggcgcagct
     4621 cctggagcgc aaccccgaaa gcatccgcgt catcgcgccc gatgtcggcg gcggcttcgg
     4681 ccccaaggcg atcacttacg gcgaggatgc ggcggtcgcg gcggcggcgc tgcggctcca
     4741 acgcccggtg aaatggatcg aagaccgccg cgagcatttc ctctccacca cgcaggaacg
     4801 cgaccaatat tgggacatcg aaatcgcgct cgatgccgat gcgaaattgc tgggcgtgcg
     4861 cggcaccatg ctgcacgaca ccggcgcgta cctgccctgg ggcgtggtga tgccttacat
     4921 ctccgcgacc acggtacccg ggccctatgt gcttccggcc tatgagctgg aggtcaccgt
     4981 cgcctatacc aacaaggttc caacgacgcc ggtgcggggc gcgggccggc cgcaagccgt
     5041 ctttgcgatg gagcgtctga tggaccgcgc cgcgcgtgag ctgcatctgg acgggaacga
     5101 aatccgccgc cgcaatttga tcccgccgaa ccggatgccc tatgcggtcg gccttatttt
     5161 ccgcgacggg aagcccgtga tctatgacag cggcgattat ccggccgcac aggatcacgc
     5221 gttggcgctc tcggcctatg atgattttcc gcgccgccag aacgaggcgc gcgaacgggg
     5281 ccgttatatc ggcatcggca tcggctccta tgtcgaaggc acaggcctcg gtccgtttga
     5341 aggtgttacc gtgcgcgtgc tcgacgacgg cagcgttcag gtcgcatcgg gggcggcgcc
     5401 gcaggggcag gggcaccaga cgctgctcca gcagatcgtg gcggatcagc tcggcgtcgc
     5461 aatggaaaac atccgcgtca cggtgggcga cacgggggca atcgcgatgg gcatcggcac
     5521 gttcgccagc cgcatcacgg cgaatgcggg accatcggcg tttattgccg ggcaggcggt
     5581 gcgcaagaag atcctcgatc tggcggcgcg cgtgctcgat gccgatgtcg cggatttgca
     5641 tatcgaagat agccgcgtcg aagtgaagtc gggcaaccgg cgcgtggttt ccttcgccga
     5701 tcttgcccgg atggcgcaag gcttccccgg cttttccttt gcgccgggcg agacgccggg
     5761 cctcgaacac accgcctatt tcacgccgcc gcgcgccgcc tattgcaacg gcacccatgt
     5821 ggccgaagtg gaagtcgaca tcgaaacctg acgtgtgcgc attctgaaat acagcgtcgc
     5881 gcatgatagc ggcacgctga tcaatcctct gatcgtggac gggcagattc agggcggcgt
     5941 cgcgcatggc atcggcaacg cgctgctgga attcatggct tatgattcca acgcgcagcc
     6001 gctgaccacc agttttgccg attatctgct ccccggggcc tgcgacgtgc cggggatcga
     6061 cgcggtgcat ttggaaacgc cgtcgccgct caatccgctg ggcgtgaagg gcgcgggcga
     6121 gggcggcacg attcccgccg ccgccgccct catcgcggcc atcgagaatg cgctttcgcc
     6181 attcggtgtg cggctgacgg aatcgccgat cacgcctgcg cgtatcgtcg ccctcttacg
     6241 gcaaaacaga ggttctgcat ctctgatgcg gcaagagctt gacgcgaaat ccgagcttca
     6301 aattcgacgg acaatcccat aatgccgtat ggtccaggac accgcggtca agaggaagtc
     6361 aggggtgaaa cgatgaggat cacgagacgg gaattcggtg cgctttcggg ggcagtattg
     6421 gcggcgggcg cgttttcggc ccaggccgat gcgggtgagg ccgcggccat gccgcccggc
     6481 gcggtggatt gccacaatca cgtcatgggg gcgagcgcca aatatcccta tgccgcggag
     6541 cgcacctaca cgccgccgga agcccccgtt gccgcgctga aagcgctgcg ggccgagatc
     6601 ggcacggcgc gcaatgttct ggtgacgccg agcgtctacg gcacggacaa tcgctgcatg
     6661 gtggacgcgc tggccgaact cgcagggacg gcgcgcggca tcgcggtctt gccgctcgat
     6721 gtgtccgatg ccgagctgca tcggctgaac gcggcgggcg tgagaggtat ccgcatagcc
     6781 ctgggcggaa ccggccgcta cgattccaaa accgcgcccg cagccctcgc gtctttcgcc
     6841 acgcgctttg cgccgctcgg atggaatatc gaaatggtcg gcaatctcac cgcgctgatg
     6901 ccgatggcgc cggcgatggc cgatctgaaa gtgcccgtgg tgctcgatca ttttgccggc
     6961 gccaacggcg gggacggggt caagcaggac ggttttgccg cgctgttgga actggtccgt
     7021 ggcggcaacg cctatgtgaa gctgtccgcg ccttataacc ggtccaagcg cgcggatttt
     7081 tccgacatgc tgccgcttgc gcgcgcgctt ctcgaaaccc gccccgatcg catgctgtgg
     7141 ggcaccaatt ggccgcatcc cgaccagatc ccggggcgcc cgatcagcga gatcacgccc
     7201 tatcatgtgg tcgacaacaa aaatctcgtg cggctgttcg cggaatggtg ccccgatgcc
     7261 ggcatgcgca aaaccatcct ggtcgatacg ccggtcaggc tctaccgctt ctcgtgacca
     7321 tgatggaggt ccgcaaactg ggtgcggcca tgggcgcgga gatcctgggt gcggatctga
     7381 acgcgctctc atccgatgat ttcgcccaaa tccgcgacgc gtttcatacc catcaggtga
     7441 tcgccatccg cgatcagacc ctttcgccca cggctcagat cgatttctcg cggcgtttcg
     7501 gcgcgctcga agatcagctc aacgcccatt acaccgtgcc ggattatccc gaggtgctgg
     7561 tcctttccaa cgatgtcaag gacggcaagc cgatcgggct gatcgacggc ggcgatttct
     7621 ggcactcgga ttcctcacac cgcgaatgcc ccagcatggc gaccatcctt tacgcggtga
     7681 agaatccgcg tcagggcggc gacaccgaat tcgccagcat gtacgccgcc tatgattcgc
     7741 tcgcccccga catgaagcgt cgcatcgcgc cgctgaaggg catccacgcg gtcagcaagc
     7801 tcaagaataa gcgcgtgacg gtttcacccc gccggcctga cgggaaggac ttctacgaga
     7861 aacaaaaatc gcttcccgat cagagttggc ccatggtgcg cacgcacccg gtcacgggac
     7921 gcaaggcgct gtatgtcagc ccgcgcttca ccatcgggat cgagggcatg gacaccgctg
     7981 aggccgatga aatcctggat gatctcttcg cccatcagat ccggcccgaa ttcgtctatc
     8041 gccatcactg gcagaatcgc gatctggtga tgtgggacaa tcgcgcggtg atccatcagg
     8101 caaccggcgg ttatgtctat cccgatgtcc gcaccatgta tcgcacggtg gtcgccggcg
     8161 accggccgtt ctgaattgtg tcaagcggtg ttttgggcgg tacccggcaa taacattcgc
     8221 gtcaagcctg cctgcaatcg cattcctgtt tagggttccg aaagcccggg ctgttacact
     8281 gggtgacatg ccggacccgt ttcccttggc ttggccgggc tttgccgcta tgtctcaccc
     8341 cccgcagggg
//
LOCUS       DASYEG010000235         4395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4433098, whole genome shotgun sequence.
ACCESSION   DASYEG010000235 DASYEG010000000
VERSION     DASYEG010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4433098"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..443)
                     /locus_tag="VGM72_10225"
     CDS             complement(117..443)
                     /locus_tag="VGM72_10225"
                     /inference="COORDINATES: protein motif:HMM:NF016280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEY4265684.1"
                     /translation="MQTDNRVLDGMARFFTNAAGAAQAFRAEMETMIRARVERLIADF
                     DFVPREEFDAVKAMAAKARTENDRLTARVIQLEMKLGVAPPKPGRTQRASRRAKPLAG
                     RRRGKL"
     gene            643..1533
                     /gene="lgt"
                     /locus_tag="VGM72_10230"
     CDS             643..1533
                     /gene="lgt"
                     /locus_tag="VGM72_10230"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY4265685.1"
                     /translation="MIHAILTYPRIDPVLIHIYGPISIRWYALSYIAGLLLGWGYVVR
                     LLRTPRLWAGRPFMGKPPATPDDIGDLFVWVTLGVIIGGRLGFVLLYGMLYCGFAGQG
                     APACVGLPEAYIANPLKILAAWEGGMSFHGGLIGVVLAILLFCRSRKLPLLAIADLIA
                     AATPIGLFFGRIANFINGELWGKITDVPWAMVFPNALPPGVPRHPSQIYEALMEGMLL
                     FFILRILIVNFQVHRRPGMVVGWFLTFYGLFRFIAEFFRDSESKLYGWFSMGQALSLP
                     MWAAALLFFWYALGPVEAKQ"
     gene            1530..2585
                     /locus_tag="VGM72_10235"
     CDS             1530..2585
                     /locus_tag="VGM72_10235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY4265686.1"
                     /translation="MNPLKEKILRLVRETGPITLAHYMQMALLDPKHGYYVKRDPLGR
                     DFITAPEVSQIFGELIGLSFVQAWEDRGRPERFCLVELGPGRGTLMADLLRAAAKVRP
                     DFAAAADIVLVEVSPALRAAQARALQDHAVRWASALGDVPDDAPLFLIANEFFDVLPA
                     RQFVRSAEGWHERMIAERDGTLTFVLSPEIAARTLPQAVPNQVFEIQDAASAIVAEIA
                     ARIVSRSGAALIIDYGHFAGLGDTFQAVKAHEYCDPMENPGEADLTVHVDFASLFEAA
                     RHENAQVLGPINQGAFLDALCIRLRAERLKCAAPERRTEIDAAIDRLTNPAHMGSLFK
                     VMAICDGRSPGMPGFPC"
     gene            2579..3346
                     /gene="pgeF"
                     /locus_tag="VGM72_10240"
     CDS             2579..3346
                     /gene="pgeF"
                     /locus_tag="VGM72_10240"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEY4265687.1"
                     /translation="MLILKGQDLSARNIAHGFFGRQGGVSEGIYESLNCGPGSRDSRD
                     AVMENRARARSALASDARLVTLYQIHSAQAVSVTQAWDIADNPKADAMATNRPGIMLG
                     ILTADCAPILLCDPGAGVIGAAHAGWNGALSGVTDSVVAAMVGLGAETRHIRAAIGPC
                     IGQAAYEVGPEFEARFRAVDPDNARFFAASARAHHWQFDLPAYVAHRLRNAGVESVEM
                     LGACTYDRETDFFSYRRATHRKETDYGRQLSAIMLRD"
     gene            3455..4387
                     /locus_tag="VGM72_10245"
     CDS             3455..4387
                     /locus_tag="VGM72_10245"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008307606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEY4265688.1"
                     /translation="MKLLAGNSNRLLAEAIGQYLHVPFTSAIVRRFADMEVFVEIQEN
                     VRGEDMFVIQSTSFPANDNLMELLVMIDALKRSSAKRITAVIPYFGYARQDRKPGPRT
                     PISAKLVANLITQAGASRVLTVDLHAGQIQGFFDIPTDNLYGQPVMVKDIQDYLNGEH
                     LMVVSPDVGGVVRARALAKRLGADLAIVDKRRERAGESEVMNIIGDVSNRTCLLVDDI
                     VDSGGTICNAAEALLEEGAKAVYAYATHGVLSGGAVARIRDSKLKSLVITDSIAATED
                     IRKCPNIRRISIATLLGEAIDRIAHEKSVSSLFD"
BASE COUNT          774 a         1323 c         1374 g          924 t
ORIGIN      
        1 tcctcttacg ccaacggggc gcgaagcgat ctcatgtcag gagacacaag attttgattc
       61 ctgacaaggg cactacacgc aggcttgtga atcaagccgt gcatgagcat gggaattcac
      121 agctttccgc gccggcgtcc ggcgaggggc ttggcgcgtc gcgatgcgcg ttgcgttcgc
      181 ccgggcttgg gtggcgcgac gccgagcttc atttcgagct ggatcacgcg ggcagttagt
      241 ctgtcgttct cggttcgcgc cttggcggcc atggccttca ccgcgtcgaa ttcctcgcgc
      301 ggcacgaaat cgaaatctgc gatcagacgc tcgacgcgcg cccgaatcat ggtctccatt
      361 tcggccctga aagcctgtgc cgcacccgcg gcgttggtga aaaaacgcgc catgccgtcg
      421 agaacgcgat tgtcggtctg catgcttcct attcctcaaa cgggatatgc gcctgggccc
      481 gcttccgatg caagccggat cgttcataaa cgtaaacatc cccgcccgcg tatcaagcag
      541 gtgaggccaa aacttgacag gaacgacgcc tggcagggcc aagcttgaca gcaggccgcc
      601 gctcgcgcga gctaaccaag acccgcatta cccgcaagcg ccatgatcca cgccatcctg
      661 acctatccgc gcatcgatcc agtcctgatc catatttatg gcccgatcag cattcgctgg
      721 tatgcactgt cctacatcgc gggtctgctg ctcggctggg gatatgtggt caggcttcta
      781 cgcaccccca gattgtgggc gggcaggccc ttcatgggca agccaccggc caccccagac
      841 gatatcggcg atttgttcgt ctgggtgacg ctcggcgtca tcatcggcgg gcggctgggc
      901 tttgtactgc tttatgggat gctctattgc ggctttgccg ggcagggcgc tcccgcctgt
      961 gttgggctgc cggaagccta tatagccaat ccgctgaaga tcctcgcggc ctgggaaggg
     1021 ggaatgtcct tccatggcgg cttgatcggc gtggtcctcg cgattctcct gttctgccgc
     1081 agccggaaat tgccgcttct ggcaatcgct gacctgattg ccgcggcgac gccgatcggc
     1141 ttgttctttg gacgcatcgc caatttcatc aatggcgaat tatggggcaa aatcacggat
     1201 gttccgtggg ccatggtgtt tccgaacgcg ctgccgcccg gcgtgccgcg ccatccgagc
     1261 cagatctatg aagcgctgat ggaaggcatg ctgttgtttt tcatcctacg aatcctgatc
     1321 gtgaattttc aggtccatcg ccggcccggc atggtggtgg gatggtttct caccttttat
     1381 gggctgtttc gtttcatcgc cgaattcttc cgcgactctg aatccaagct ctatggctgg
     1441 ttcagcatgg gccaggccct cagcctgccg atgtgggcgg cagcgttgct gttcttctgg
     1501 tacgcgctcg gccccgtcga agcgaaacag tgaatcccct gaaggagaag atcctccggc
     1561 tggtgcgcga gaccgggccg atcacgcttg cgcactatat gcagatggcg cttctcgatc
     1621 ccaagcatgg ttattatgta aaacgcgatc ccttgggccg cgatttcatc actgctccgg
     1681 aagtcagcca gatattcggc gagctgatag ggctcagctt cgtccaggcc tgggaggatc
     1741 gcggccgccc cgagcgattt tgtctcgtgg agcttggccc cgggcgcggc acactgatgg
     1801 ccgacctgtt gcgggcggcc gcgaaagtgc ggcctgattt tgctgccgcc gcggacattg
     1861 tgctggtgga ggtgagtccg gcgctgcgcg ccgctcaggc gcgcgcgctt caggatcacg
     1921 cggtgcgatg ggcatcggca ctgggcgatg tgccggatga cgcgccgttg tttctcattg
     1981 ccaatgaatt cttcgacgtg ctcccggcgc gccaatttgt gcgctccgcg gaaggctggc
     2041 atgagcgtat gatcgcagag cgcgacggca cgctcacatt cgtgctgtcc cccgaaatcg
     2101 cggcaagaac attgccgcag gccgttccca accaggtgtt tgaaatccag gacgccgcaa
     2161 gcgcgatcgt cgcggagatc gcggcccgga tcgtgtcgcg cagtggcgcc gctctcatca
     2221 tcgattatgg gcatttcgca gggctaggcg atacgttcca ggccgtgaaa gcccatgaat
     2281 attgcgatcc gatggaaaat cccggggaag cggacctgac cgtgcatgtc gatttcgcgt
     2341 ccttgttcga agcggcgcgg catgaaaatg cgcaggttct gggccccatc aaccaaggag
     2401 cattcctcga tgcgctttgt atccgcttgc gggcggagcg cctcaaatgt gcggcgccgg
     2461 aaagacgcac ggaaatcgat gccgccattg accggctgac gaacccggcg cacatgggaa
     2521 gcctgttcaa ggtcatggcg atttgtgacg ggcgttcgcc cggaatgccg ggttttccat
     2581 gctgatcctc aagggccaag atctttcggc gcgcaatatc gcgcacgggt ttttcgggcg
     2641 ccaaggcggc gtgtcggaag gaatctacga atcgctcaat tgcggtccgg ggtcgaggga
     2701 ttcgcgcgac gcggtgatgg aaaaccgcgc ccgcgcccgc agtgccctgg cttccgatgc
     2761 gcggttggtc acgctctatc agattcatag cgcgcaagct gtgagcgtca cgcaggcatg
     2821 ggacattgcc gacaatccca aggccgatgc catggccaca aatcgtcccg gaatcatgct
     2881 cgggattctt accgccgatt gcgcgccgat cctgttgtgc gacccgggag caggggtgat
     2941 tggtgcggcg catgccggct ggaatggcgc gctgtcgggt gtgaccgatt cggtcgttgc
     3001 cgccatggtg gggctgggcg cggaaacgag acatatccgc gccgccatcg gtccctgcat
     3061 cggccaggcc gcctatgagg tcggccccga attcgaggcg cgctttcgcg cagtcgaccc
     3121 ggacaatgcg cgtttcttcg ccgcttcggc ccgcgcgcac cattggcagt tcgatcttcc
     3181 ggcctatgtg gcgcatcgct taaggaatgc cggcgtggaa tccgtggaaa tgcttggcgc
     3241 ctgcacctat gaccgcgaga cggatttttt ctcctaccgc cgcgcgaccc atcgcaagga
     3301 gaccgattac ggccgccaat tatcggccat tatgctgcgg gattgagccg cgtgatttgt
     3361 cctctttgtg gaggtttacg cactcggtgt gccttgctag aagagccgga gggcgcgtga
     3421 gtcacagaat cgtcatgagg gttggggtcg ttgcatgaag ctgctggccg gcaacagcaa
     3481 tcgtttgctc gccgaagcga tcggccagta cctgcacgtt ccgttcacga gtgcgatcgt
     3541 gcggcgtttc gccgacatgg aggtcttcgt cgaaatccag gaaaatgtcc gcggcgagga
     3601 tatgttcgtc atccaatcga cgagctttcc ggccaacgac aatctgatgg aattgctggt
     3661 catgatcgac gcgctcaagc gctcgtcggc caagcgcatt accgcggtga tcccctattt
     3721 cggctatgcg cgccaggacc gcaaacccgg gccccgtacg ccgatttccg cgaaactggt
     3781 cgccaatctc atcacccagg ccggtgcctc gcgtgtgctg acggtcgatc tgcatgccgg
     3841 ccagatccag ggatttttcg atatcccgac ggacaatctc tacggccagc cggtgatggt
     3901 taaggacatc caggattatc tcaacggtga gcatctgatg gtggtgtcac ccgatgtcgg
     3961 cggcgtggtg cgggcgcggg cgctggccaa gcggctgggc gcggacctcg ccatcgtcga
     4021 caagcgccgc gagcgcgccg gagagtcgga ggtcatgaat atcatcggcg atgtgtcgaa
     4081 ccggacctgc cttctggtcg acgacatcgt ggattccggc ggcaccatct gcaatgcggc
     4141 cgaagcgttg ctggaggagg gggccaaagc cgtctatgcc tatgccaccc atggcgtgct
     4201 gtccggcggc gcggtcgccc gcatccgcga ctctaagctg aagtcgctgg tcatcaccga
     4261 ttccatcgcg gcgaccgagg acatccgcaa atgcccgaac atccggcgga tctccatcgc
     4321 cactttgctg ggtgaggcga tcgaccggat cgcgcatgag aaatccgtct ccagcctgtt
     4381 cgactgattt ttccg
//
LOCUS       DASYEG010000236         2162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2547705, whole genome shotgun sequence.
ACCESSION   DASYEG010000236 DASYEG010000000
VERSION     DASYEG010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2547705"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1551)
                     /locus_tag="VGM72_10250"
     CDS             complement(<1..1551)
                     /locus_tag="VGM72_10250"
                     /inference="COORDINATES: protein motif:HMM:NF001964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEY4265689.1"
                     /translation="MDTALSAPDSTASAQTSGLTTGLAAEVERRRTFAIISHPDAGKT
                     TLTEKLLLFGGAINLAGQVKAKRDRRSTRSDWMAIERERGISVVTSVMTFDYDSRVFN
                     LLDTPGHEDFSEDTYRTLTAVDSAVMVIDGAKGIEARTLKLFEVCRLRDIPIITFINK
                     MDRETRDPFELLDEIEKTLALDTTPMNWPIGRGRDFAGTYEIATGDMRLLNDDGRAAV
                     PQPLDLASLHAKNSMLDEKAIAEELALVKDASRPFDFAAFREGHLTPVYFGSAIKNFG
                     VKDLLDALASFAPPPRPQTANTRKVEPQENGMTAFVFKIQANMDPNHRDRIAFARLCS
                     GKLRRGMKAKLVRTGKPMTLSAPQFFFAQDRSVADEAYPGDVVGIPNHGTLRIGDTFT
                     EGEEITFLGVPNFAPEILRRVKLGDPMKAKKLKEALQQMAEEGVVQVFRPLDGAPALV
                     GVVGPLQLDVLKTRLAAEYGLDIGFEQPEFQLARWIAHDDPKKLDEFVSGNYSSVAED
                     LDGDRVFMA"
     gene            complement(1647..2108)
                     /locus_tag="VGM72_10255"
     CDS             complement(1647..2108)
                     /locus_tag="VGM72_10255"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY4265690.1"
                     /translation="MSTRVNFYAVAPEAAKAVTALEQYCQNSGLPRRLIHLMKLRASI
                     INGCAYCVDMHSKEARRDGLSEQWINLVSTWHEARVYSEAERAVLGWTDALTLLAQTR
                     APDADYEALRAHFSEEDIAKMIVVIGTINVWNRIAVGTRMHHPVDAGAKAA"
BASE COUNT          403 a          661 c          672 g          426 t
ORIGIN      
        1 cgccatgaag acacggtcgc cgtcgagatc ctcggcgacg ctcgaatagt tccctgacac
       61 aaattcatcg agcttcttgg gatcgtcatg cgcgatccag cgcgcgagct ggaattcggg
      121 ctgctcaaag ccgatgtcga ggccgtattc ggcggcaaga cgcgtcttca agacgtcgag
      181 ctgcagcgga ccgaccacgc cgaccagcgc cggtgcgccg tccagcgggc ggaacacctg
      241 caccacgccc tcttccgcca tttgctgcaa agcttctttc agcttcttcg ccttcatggg
      301 atcgccgagc ttcacgcggc gcaggatttc cggcgcgaag ttcggcacgc cgagaaatgt
      361 gatttcttcg ccttcggtaa aagtatcgcc aatacgcaaa gtgccatgat tgggaatgcc
      421 gacgacgtca ccaggataag cttcgtccgc caccgagcgg tcctgcgcga agaagaactg
      481 cggcgcagac aacgtcatcg gcttgccggt gcgcaccagc ttcgccttca tgccgcggcg
      541 cagcttgccc gaacacaacc gcgcaaaggc gatgcggtcg cgatggttcg gatccatgtt
      601 cgcctggatc ttgaacacga aggcggtcat gccgttttcc tgcggctcga ccttgcgggt
      661 gttcgcggtc tgtggccgcg gcggcggcgc gaaactcgcc aacgcatcga gcagatcctt
      721 gacgccaaaa ttcttgatcg cggatccgaa atagaccggc gtcagatgtc cttcgcgaaa
      781 ggctgcgaag tcgaacggcc ggctggcgtc cttcaccagc gccagttctt cagcgatggc
      841 cttctcgtcg agcatcgaat ttttggcatg cagcgatgca aggtcgagcg gctgcggcac
      901 cgcggcgcgt ccgtcgtcgt tcaacagccg catgtcgccg gtggcgattt cgtacgtacc
      961 ggcgaaatcg cggccacggc cgatcggcca gttcatcggc gtggtgtcga gtgccaaagt
     1021 cttttcgatc tcgtccagca actcgaacgg atcgcgagtc tcgcgatcca ttttattgat
     1081 gaaggtgatg atcgggatgt cgcgcaggcg gcacacttcg aacagcttga gcgtgcgcgc
     1141 ttcgatgccc ttcgcgccgt cgatcaccat gaccgcgcta tcgaccgcgg tcagcgtgcg
     1201 gtaggtgtct tcggagaagt cctcatggcc cggcgtgtcg agcagattga agacgcggct
     1261 gtcatagtcg aacgtcatca cggacgtgac gacggaaatg ccgcgctcgc gctcgatcgc
     1321 catccagtcc gaacgggtcg aacggcggtc gcgcttggcc ttcacctgtc cggcgagatt
     1381 gatggcgccg ccgaacagca gcagcttttc ggtcaaagtc gtcttgccgg cgtcagggtg
     1441 cgaaatgatc gcgaaggtgc gccggcgttc gacctcggcg gccaagccgg tggtaagacc
     1501 tgaggtttgc gcagaagcgg tcgaatcggg cgctgaaagg gcggtgtcca tgccgccgca
     1561 tgtggcaggg aaaacgccct atttcaaggc ttttcccctg cgccaaggga tcaaggaggc
     1621 gcgggggaag ccttgtcagg acgctatcaa gcggctttcg cgcctgcgtc caccggatgg
     1681 tgcatgcggg tgccgaccgc gatccggttc cacacattga tcgtgccgat gacgacgatc
     1741 atcttggcga tgtcttcctc ggagaaatgc gcgcgcaacg cttcgtagtc cgcgtccggc
     1801 gcgcgggtct gcgccagcaa cgtcagcgca tcggtccagc cgagcacggc gcgctcggcc
     1861 tcggaataga cccgcgcctc atgccaggtg gagacgaggt tgatccactg ctcgctcaac
     1921 ccatcgcgcc gcgcttcctt cgagtgcatg tcgacgcagt aagcgcagcc attgatgatg
     1981 gaggcgcgca gtttcatcaa atggatcagc cgcctcggaa gaccgctgtt ctggcagtac
     2041 tgctccagag ccgtgacggc tttggccgct tcgggcgcga cagcgtaaaa attgacacgt
     2101 gtgctcatgg catcactcct tcatatggat gttcggttgc gttcgttcag atcgaaaaag
     2161 gc
//
LOCUS       DASYEG010000237         4317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1683118, whole genome shotgun sequence.
ACCESSION   DASYEG010000237 DASYEG010000000
VERSION     DASYEG010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683118"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VGM72_10260"
     CDS             <1..384
                     /locus_tag="VGM72_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY4265691.1"
                     /translation="LILADLPGYGYARVSRTDSERWTELIFAYLRGRAHLRRAVLLID
                     SRRGPMAQDIDVMDLLDRSAVSYQMVLTKVDKLKAGEHAQVESQTAAEARRHGAAHPE
                     LISTSAVMGDGIPALRAALAALAAP"
     gene            468..1394
                     /gene="argB"
                     /locus_tag="VGM72_10265"
     CDS             468..1394
                     /gene="argB"
                     /locus_tag="VGM72_10265"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY4265692.1"
                     /translation="MSVQDDGTREGNLRSLARWRSTARVLVEALPFILQYDNTTIVVK
                     YGGHAMEGGVPREFAQDIVLMKQTGINPVVVHGGGPQIGAMLKRLNIASSFVDGLRVT
                     DAAAMEVVEMVLSGTINKQIVAGINAAGGRAVGVSGKDGNMVVARKIERIKTDPKTGE
                     RTAIDLGYVGEPERVDAEVLRMILNSDLIPVVAPIGVGAKAETYNINADTVAGAVAGA
                     IAAERLILLTDVEGVLDREGKLIPHLTVSQARGLMQDGTISGGMIPKIETAVDAVEKG
                     VHAAVILDGRIPHVLLLELFTEHGAGTLITPD"
     gene            complement(1396..2694)
                     /locus_tag="VGM72_10270"
     CDS             complement(1396..2694)
                     /locus_tag="VGM72_10270"
                     /inference="COORDINATES: protein motif:HMM:NF021603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEY4265693.1"
                     /translation="MAGAFAEIVPVIGFLKRLLNTKELPARLSYDEARAVLESNRGPL
                     GRKLAARTDAEPEMLYYLAERGTPEARRNAAANPATPAAANRILAEDIDPDVRAELAH
                     KIGRLLPDLLAPERERVCELTLDALRRLASDQLVRVRKILAAEIKSHPCAPKDVILML
                     AGDVEETVSVPILEYSPLLSDPDLLEIIASARAQSALAAVARRHGVSERLSEVIVASL
                     DIGAVAALLANPNAIIRENTMERIIGHAQSIEAWQGPLVTRSDLSLRALRRIAGFVGV
                     SLLHQLTERHGLDDETVQHFKRCLRLRLERDDETAETKEDKVHAAVLAVRDRGALDER
                     YVEEAVEAGQRDTVIESLALLACAPRPAIEKILSSRSAKAITALAWKAGLSMRIAFKM
                     QTLLLKLHSDELLPARAGVAFPLAEDEMRWHLSYFQVPTR"
     gene            2845..3669
                     /gene="dapD"
                     /locus_tag="VGM72_10275"
     CDS             2845..3669
                     /gene="dapD"
                     /locus_tag="VGM72_10275"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008035733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEY4265694.1"
                     /translation="MNDSDLQQAIDAAWEGRDRLSPESRGAERDAVEAALEGLDSGRL
                     RVAGKTGGEWRVHQWLKKAVLLSFRLNDMSPIEGAAGGASWWDKVDSKFKGWGENRFR
                     EAGFRAVPGAIVRRSAYIAKNVVLMPSFVNVGAHVGEATMIDTWATVGSCAQIGKHCH
                     ISGGTGIGGVLEPLQAGPVIIEDNCFIGARSEVAEGVIVGEGSVLAMGVFLGASTKII
                     NRNNGDVHYGKVPPYSVVVPGCLPSAAGPALYCAVIVKQVDERTRAKTSINELLRD"
     gene            3742..>4317
                     /locus_tag="VGM72_10280"
     CDS             3742..>4317
                     /locus_tag="VGM72_10280"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY4265695.1"
                     /translation="MTDPVSLAQALIRCPSVTPEDSGALGVLESALKPLGFTCRRLRF
                     EMPDSPAIENLYARLGAARPNFCFAGHTDVVPVGAADGWRHDPFAAEIAGGMLYGRGA
                     ADMKSAIAAFVAATARHIAKNGGPKGSISLLITGDEEGPAVNGTVKVLDWLKQNGEAL
                     DHCLVGEPTSAARIGDMMKIGRRGSMNLRASV"
BASE COUNT          770 a         1351 c         1429 g          767 t
ORIGIN      
        1 ctgatcctgg cggatttgcc gggctatggc tatgcgcgcg tctccaggac cgattcggag
       61 cgctggacgg aactgatctt cgcctatctg cgggggcgcg cccatttgcg ccgcgcggtg
      121 ctcctgatcg attcacggcg cggacccatg gcgcaggaca tcgacgtcat ggatctgctg
      181 gaccgttcgg ccgtctccta tcagatggtg ctgaccaagg tggataagct gaaagccggc
      241 gaacacgcgc aggtggagtc gcagacggca gcagaagccc gccgtcacgg tgcggcccat
      301 ccggaattga tctccacctc ggcggtcatg ggcgacggaa ttccggcttt gcgcgctgct
      361 cttgcggctt tggcagcccc ttaaccacgc ttaacccgaa gttttggttt tgacgccggt
      421 gcgcaggctc tataagcctt ccgcacgccg acttcggggg aattcccatg agcgtgcagg
      481 acgacggcac acgcgaagga aatctgcgct cgctcgcgcg ctggcgctcc accgcacgcg
      541 tgctggtgga agcgcttcct ttcattctgc aatacgacaa cacgaccatc gtggtgaaat
      601 atggcgggca cgcgatggaa ggcggtgtgc cgcgtgaatt tgctcaggac atcgtgctga
      661 tgaagcagac cggcatcaat ccggtcgtgg tgcacggcgg cggaccgcag atcggcgcca
      721 tgctgaagcg cctgaacatc gcctcatcct tcgtggacgg cctgcgcgtg accgatgccg
      781 ccgcgatgga agtcgtcgag atggtgcttt ccggcaccat caacaagcag atcgtggcgg
      841 gcatcaatgc cgcgggcggg cgcgcggtcg gcgtctccgg caaggacggc aacatggtgg
      901 tcgcccgcaa gatcgagcgc atcaagaccg atccgaaaac cggcgagcgc acggcgatcg
      961 atctgggtta tgtgggcgaa cccgaacgcg tggacgccga ggtcctgcgc atgatcctca
     1021 attccgatct cattccggtg gtggccccca tcggcgtcgg tgccaaggcg gagacctaca
     1081 atatcaacgc cgatacggtg gcgggcgcgg tcgccggcgc gattgcggca gagcgcctga
     1141 tccttctcac cgatgtcgaa ggcgtgctgg atcgcgaggg caagctgatc ccgcatctga
     1201 cggtcagcca ggcgcgcggg cttatgcagg acggcacgat ttccggcggc atgatcccga
     1261 agatcgaaac cgccgtcgac gcggtggaaa agggcgtcca cgcggcggtc atcctggacg
     1321 gacggattcc tcatgtcctg ctgctcgaac tcttcaccga gcacggcgcc ggcacgctga
     1381 tcacgccgga ttaattcaac gcgtgggcac ctgaaaatag gaaaggtgcc agcgcatttc
     1441 gtcttccgcg agcggaaagg cgacgcccgc gcgcgcgggc agcaattcgt cggagtgcag
     1501 cttcagcaac agggtctgca tcttgaacgc gatgcgcatc gacaatcccg ccttccaggc
     1561 gagcgccgtg atcgccttgg cgctgcgcga ggacaatatc ttctcgatcg ccggtcgcgg
     1621 ggcacaggcg agcagcgcca gcgactcgat caccgtgtcg cgctgaccag cctccacggc
     1681 ttcttcgaca tagcgttcat cgagcgcgcc gcggtcgcgg acggcaagca ctgccgcatg
     1741 gaccttgtct tccttggtct cggcggtttc atcgtcgcgt tccaggcgca ggcgcaggca
     1801 gcgtttgaaa tgctgtaccg tttcgtcgtc gagcccatga cgctccgtga gctggtgcag
     1861 cagcgaaacg ccgacaaaac ccgcgatccg cctgagcgct cggagcgaca aatcgctgcg
     1921 tgtcaccagg ggtccctgcc acgcttcgat ggattgggcg tggccgatga tgcgctccat
     1981 ggtgttttcg cggattatcg cattgggatt ggcaagcaag gcggccaccg cgccgatatc
     2041 gagcgaggcc acaatgactt cggacaagcg ttcgctgacg ccatggcggc gtgcaaccgc
     2101 tgccagggcg ctttgggcgc gcgcgctcgc gatgatctcc agaagatcgg gatcggagag
     2161 aagcggcgaa tattccagta tgggaacgct gacggtttct tcgacatcgc ccgccagcat
     2221 caggatcaca tccttgggcg cacagggatg ggatttgatc tcggccgcga gaatcttgcg
     2281 cacgcggacg agctgatcgc tcgccaaacg gcggagcgca tcgagagtca gttcgcacac
     2341 ccgctcgcgt tcgggagcca acagatcggg cagcagccgg ccgatcttat gcgccagttc
     2401 ggcgcgcacg tccgggtcga tgtcctcggc cagaatacgg ttggcggccg cgggcgtcgc
     2461 cggattggcc gcagcgttgc ggcgggcctc aggcgtgccg cgctcggcga ggtaatagag
     2521 catttccggt tcggcatcgg tgcgcgcggc gagtttgcgt ccgagcgggc cccggttgga
     2581 ttccagaacc gcgcgcgctt cgtcatagga aagccgcgcc ggcagctcct tggtgttcag
     2641 caggcgtttc aaaaatccga tcaccggaac aatctccgcg aatgcaccgg ccacatctct
     2701 cttccggcgg gagccaattt tgcatgaggc cgcttaaggg agcgctaacc tgcaggtcca
     2761 atcgcctggc gcctcttgcc agaaacgggg cgctcgccca tgttcggccc atctttcccg
     2821 cacctcgtta acggatgcta caccatgaat gacagtgacc tgcagcaagc tatcgacgcg
     2881 gcctgggagg gacgcgaccg gctttccccc gaatcgcggg gagccgagcg cgatgcggtc
     2941 gaggccgcac tcgaaggcct cgattcgggg cggctccggg tggcgggaaa aaccggcggg
     3001 gaatggcgcg tccatcaatg gctgaagaaa gcggttcttc tgtctttccg cctgaacgac
     3061 atgagcccga tagagggcgc agccggcggc gcctcctggt gggacaaggt cgattccaaa
     3121 ttcaagggct ggggcgaaaa ccgcttccgc gaagcggggt tccgcgccgt gccgggcgcc
     3181 atcgtgcggc gctccgccta tatcgcgaaa aacgtggtgc tcatgccgtc cttcgtcaat
     3241 gtgggcgccc atgtcggcga agccaccatg atcgacacct gggcgacggt cggctcctgc
     3301 gcacagatcg gcaagcactg ccacatttcg ggcggcaccg gaattggcgg ggtgctggag
     3361 ccgctgcagg cgggaccggt catcatcgaa gacaattgct tcatcggcgc gcggtccgaa
     3421 gtggccgaag gcgtgatcgt gggcgaaggc tcggtgctgg cgatgggcgt gtttctgggc
     3481 gcctcgacca agatcatcaa ccgcaacaat ggcgatgtgc attacggcaa ggtgccgccc
     3541 tattcggtgg tggtgccggg ctgtctgccc tcggccgccg gtccggcatt gtattgcgcg
     3601 gtgatcgtga agcaggtcga cgagcggacc cgcgccaaga cctcgatcaa cgaattgctc
     3661 cgcgattgag cgcgagggcc caattgaccc cggcagaccg gcggccctaa atccgcccat
     3721 gccggctgac agagcccacc cgtgaccgat cccgtttccc tggcgcaagc cctgatccgc
     3781 tgcccgtccg tgacgcccga agattcggga gcgctgggcg tgctggaatc cgcgttgaag
     3841 ccgctcggct ttacctgccg ccgtttgcgc ttcgagatgc cggattcgcc cgcaatcgag
     3901 aatctctatg cgcgtctggg tgcggcgcgg cccaatttct gtttcgccgg tcacaccgat
     3961 gtggtgcccg tgggtgctgc cgatgggtgg cggcacgatc ccttcgcggc cgagatcgcc
     4021 ggcggcatgc tctatgggcg cggcgccgcc gacatgaaaa gcgccatcgc cgccttcgtt
     4081 gcggccaccg cgcgccatat cgcgaaaaac ggcgggccca agggctcgat cagcctgctc
     4141 atcaccggcg acgaagaagg tcccgcggtg aacggcaccg tgaaggttct ggattggctg
     4201 aaacagaacg gcgaagcgct ggaccattgc ctggtcggcg aaccgacctc cgcggcacgc
     4261 attggcgaca tgatgaagat cggccgccgc ggcagcatga atttgcgcgc gagcgtg
//
LOCUS       DASYEG010000238         2319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_85272, whole genome shotgun sequence.
ACCESSION   DASYEG010000238 DASYEG010000000
VERSION     DASYEG010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85272"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="VGM72_10285"
     CDS             <1..866
                     /locus_tag="VGM72_10285"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY4265696.1"
                     /translation="RLAHALALGASRAAVPAIVRTGHISAAKSIANALIAQSGVQAAL
                     FAEAGLTGPSAILDDPRGLRELFAQDRLSILTAPMAGDGAIMRSHVKAYPCINTGQSA
                     IAAALKLHAMLDGPAEDLDRIEIVMADYPVIKRQQDDEGRTRPQSREAADHSFPFLVA
                     VTLIDGAFGIAQFDRERWRDPRVNALMEKIIMRRDPEWTMRAPGSFPCMLYAQTRRGR
                     AFTSEVSYPPGFSKDGIGETPAIEKFHAVTAPHLRESARSQIVDAVMEFHHSRSTIAL
                     DSAIGIEGTIP"
     gene            863..1594
                     /locus_tag="VGM72_10290"
     CDS             863..1594
                     /locus_tag="VGM72_10290"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY4265697.1"
                     /translation="MSEDILDQCDGNILTITLNRPDQGNALNDTGIAHLGTLLQGAAG
                     RARLVLLKAAGDDFCTGRLAPRGTAAPKLEALQVRHGRDVVFNCYAAFRESPIPVIAA
                     VQGRALGFGCAIACCADITIAADNAELQMPEFGANIMPTIAMSALLGKVTAKQLMYIA
                     YSTAVLSAERAERLGLVSEVAPGNALDAAVQRICAQILKAPQPAVLAIKEYARVAPSM
                     DIRGAVDYARSLHAVVNSSSEMRRQ"
     gene            1809..>2319
                     /locus_tag="VGM72_10295"
     CDS             1809..>2319
                     /locus_tag="VGM72_10295"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEY4265698.1"
                     /translation="MSKFLTRAAGSLAAVAFFAASAYAQAPAPAAGGDGPPVLASKLA
                     PAKGGSLRMLVTSSVFSSGGELDDKFTQNGDNMSPSLSWSKGPAGTQSYAVLVEDAGV
                     NRHDPIVHWVIYNLPSTASSVPQNVPADATLENGAMQGKNVRGAVGYIGPKPPAGQTH
                     PYHFQVFALN"
BASE COUNT          445 a          753 c          695 g          426 t
ORIGIN      
        1 cacgccttgc tcacgcgctt gcgctcgggg cttcgcgcgc cgccgtaccg gcgattgttc
       61 gcaccggcca catctccgcg gcaaaatcca tcgccaacgc tctgatcgcg caatccggcg
      121 tacaggcggc gctgtttgcc gaagccgggc tcaccggacc ttccgcgatc ctggacgatc
      181 caaggggctt gcgcgaattg ttcgcgcaag accgcctgtc catcctgacc gcgcccatgg
      241 ccggtgacgg cgcgatcatg cgttcccatg tcaaagccta tccctgcatc aacaccggac
      301 aatccgcgat tgccgccgcg ctgaaactcc acgcgatgct ggatggtccg gcagaagacc
      361 tggaccgcat cgaaatcgtc atggccgatt atcccgtcat caagcggcag caggacgatg
      421 agggccgcac ccgtccacaa tcgcgcgagg cggccgatca cagtttcccg ttcctcgtcg
      481 ccgtgacgtt gatcgacggc gcgttcggca tcgcgcaatt cgaccgcgag cgctggcgcg
      541 acccgcgcgt gaacgcgctg atggagaaaa tcatcatgcg ccgcgatccg gagtggacga
      601 tgcgcgcgcc cggcagtttt ccctgcatgt tgtacgcgca gacccggcgg ggccgcgcgt
      661 tcacgtcgga ggtttcctat ccgcccggct tctcgaaaga cggcatcggc gagacccctg
      721 cgatcgaaaa attccacgcc gtcaccgcgc cgcacttacg cgaatccgcg cgctcccaaa
      781 tcgtggacgc ggtcatggaa ttccaccata gtcgatccac aatcgcactc gactccgcaa
      841 tcggcataga aggaacaatc ccttgagcga agatattctg gaccaatgtg acggcaatat
      901 cctgaccatc acgctgaacc ggcccgatca gggcaacgca ttgaacgata ccggaatcgc
      961 ccatttgggc acgctgttgc agggcgcggc ggggcgcgcg cggctggtgc tgttgaaagc
     1021 ggcaggcgat gatttctgca ccggccggct tgcgccgcgg gggacggcgg cgccgaaact
     1081 cgaagcgctg caagttcgtc acggtcgcga cgtggtgttc aattgctatg ccgcgtttcg
     1141 cgaatcgccc attcccgtga tcgcagccgt gcaggggcgc gcgctcggtt ttggctgtgc
     1201 catcgcctgt tgcgcggaca ttaccatcgc ggcggacaac gccgaacttc agatgccgga
     1261 attcggtgcg aacatcatgc ccaccatcgc gatgtcggcg ctgctcggga aggtcacggc
     1321 gaagcaattg atgtacatcg cctattctac cgctgttctg agcgcggagc gcgcggaacg
     1381 cttggggctc gtcagcgagg tcgcgccggg caacgccctc gatgcagcgg tgcagcgaat
     1441 ctgcgctcaa atcctgaaag cgccgcaacc cgcggttctg gcgatcaagg aatatgcaag
     1501 ggtggccccc tcgatggata ttcgcggcgc cgtcgattac gcgcgcagcc tgcacgcggt
     1561 tgttaactcc tcaagtgaaa tgaggcggca atagggcaaa gtatcgcaaa gtgtgacgcc
     1621 gaatctcatt tgacatagat tcgacagtcc tcacacggat gaagtgcgac aagagttcgc
     1681 aggcgatcat gaccgcgacg taaggccaca cgaaggtttg attaatctcg ttgggttgta
     1741 ctggtcgatg cgcacggcac tcgtgctata agccgcgccc gcaaccccga tgcagagacc
     1801 caattccaat gagcaagttc ttgaccaggg cagcaggtag tttggctgcc gtcgcctttt
     1861 tcgccgcgtc tgcttacgca caagcccccg ctcctgcggc gggcggtgac ggtcccccgg
     1921 tccttgcgag caagcttgct cccgccaaag gcggcagctt gcggatgctc gtgacgtcga
     1981 gcgtcttctc ctccggcggt gaactcgatg acaagttcac gcagaacggc gacaacatgt
     2041 ctccgtccct ttcgtggtcc aagggcccgg ctggtacgca atcctatgcg gtgctggtcg
     2101 aggacgcagg cgtcaatcgt catgatccga tcgtgcactg ggtcatctac aacctgccgt
     2161 ccacggccag tagcgtcccg cagaacgttc ccgccgacgc gacgctggaa aacggcgcga
     2221 tgcaaggcaa gaacgtgcgt ggcgccgtcg gctatatcgg ccccaagccg ccagccggtc
     2281 agacccaccc ctaccacttc caggttttcg cgctgaaca
//
LOCUS       DASYEG010000239        18865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4866527, whole genome shotgun sequence.
ACCESSION   DASYEG010000239 DASYEG010000000
VERSION     DASYEG010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 18865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18865
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4866527"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="VGM72_10300"
     CDS             complement(<1..1089)
                     /locus_tag="VGM72_10300"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEY4265699.1"
                     /translation="MVVLSWMWGIVKSVLNGIAKAAVAAIILVLVLAGVAMFSGDGVP
                     RNAVLELDLRNAMDDKSTSSLFDLNGKLSIMDVVMGLDRAARDSRVKGVFLRVGSGDL
                     SVPKAEELRDALKHFEESKKFVIAHSQSFYSSGLGDYTAAAAADQIWMQPVSTFFSAG
                     AASTTMFFKGLFDKVQATPQFVQRYEYKNAANVFTETDFTPAHREATMRVLQSWYDSA
                     TAEAAADRKLDRAALISVLDASPATVETVKDKGLITNIGYDDDARDAAKARAGAGAAI
                     MRFERYAQATRNRNAGSDGASTIALVHAAGDIVEGDDDSPLSSAGNSVAGDIFSQAIR
                     DAAADKNVKAILLRVDSPGGSAIASDQIL"
     gene            complement(1268..1606)
                     /locus_tag="VGM72_10305"
     CDS             complement(1268..1606)
                     /locus_tag="VGM72_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013574351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY4265700.1"
                     /translation="MKFIVAIIQPYRLDTVREALNDSGISGLTVSEVRGYGRQRGHKE
                     VYRGAEYSINYVPKLKLEIAVDDEKADEVTETIMRTARTGKIGDGKIFVFDLVSVTRI
                     RTGEIGSQAL"
     gene            complement(1603..2814)
                     /locus_tag="VGM72_10310"
     CDS             complement(1603..2814)
                     /locus_tag="VGM72_10310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY4265701.1"
                     /translation="MLTSTVLVLFMTLPGLALFYAGLVRAKNVLSVLMHCVAVASLIS
                     VLWYAGAYSLVFVGGNAWIGDLSQAFLIGVGRNAVHAGTTIPDSVYIMYQMTFAIITP
                     GLIIGAYVERIKFGAVLLFSGLWLMVVYVPVAHWVWGGGWLAAMGVKDFAGGIVVHTT
                     AGISALVIAAALGKRDGFPHQLQPPHAPWIVMVGAAMLWVGWFGFNAGSALTAGADAG
                     MAMLVTHMAAAVASLTWMAIEWTRFGKPSLVGTVTGTVAGLATITPASGFVGPFGALV
                     IGVVSSTICYCTVHLVKTRFTVDDALDVFAVHGVGGMTGTLLTAILMPVALGGVGYGE
                     GVTMGSQFGVQALGVAATAAWSGVASLILVKITQALVGLRVGRDAETQGLDLTSHGET
                     GYNVAFGGTSQ"
     gene            3150..3629
                     /locus_tag="VGM72_10315"
     CDS             3150..3629
                     /locus_tag="VGM72_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265702.1"
                     /translation="MRRENRTISVMTVLGVGVAVALSGQSARSAPATTPQARAEAAFQ
                     AMGGDKQEMVTSIVLKGTMAQWEPGESFSVADLTMPDGGTSTFTETLDRTKGYQRIEW
                     VRPRAAGGTRSYAEIIMPDGGYVIGNDANGAPPKRDQHARQSAGAYDVRHPAHRHAA"
     gene            3595..4779
                     /locus_tag="VGM72_10320"
     CDS             3595..4779
                     /locus_tag="VGM72_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4265703.1"
                     /translation="MSAIRLTATLREIERNDIVLAMHEHPELISEAPAQTIGGKSYPA
                     VQFRGDYGSFTVAFDPDTHLPAMVRTRDFDQLMGDADFDESFSDWRDVDGLKIAHRMT
                     YTLNGMKIFDTTLNDARVDIVGNSILPADTFAVPTAIRGKAPKPSSVDKPPYQWILRR
                     LASGFFLDSDNLYTDDGGSLTLSDVAPNISLVTGGTHNTLIVATNTYLVAFDSPGDDG
                     QSKLAIDLAKQKYSGKPFRYLVLTHHHIDHSGGLRAYIAEGATLVVGKGDGAFFRKVL
                     MAPQTLNVNKPAGAVTPKIIEVAGKWSVKDGGREIDAYVIDNPHAADYLMPYVPDAKL
                     GFITDLWNPGPPVAAANPNLIAVVKGVEKWMLMPERFAGGHGAVGNYPDVMQGAAGAN
                     TR"
     gene            4885..6579
                     /locus_tag="VGM72_10325"
     CDS             4885..6579
                     /locus_tag="VGM72_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4265704.1"
                     /translation="MLKKSTIITALFGAAGISVVLGLGTVAWGQAPGTPGSGDAGAGV
                     GVLSTGKGPHALADAAYKAMGLTDEKLAPAVVTLIVKGTLQAWDPGESESLSDPYKPD
                     WGTSTYTESFDRARGLYRIEWMRPRAGGGMRNYTEILTDEYSPSGEQMGGYVMGVDVN
                     GGQPMRAVMVNGMPAHQMSGKRLTVTLRELERDAVLGDMHEHPERVSEIADQRAGGKT
                     YKAAQYRADHGTFIVLFDPASHLPAVVRTRDWDVYYGDSDYDETLSDWRDVGMGIKLP
                     FHKLITINGIKIFDEKLDNVRLNPQIAVDAFTVPAVLRRGAPAAAPTGQVPYQWILRR
                     MGNGFYLDSDALYTDDGGSLKLVDIAPNVSMVQGGSHNTLIVATNTYLVAFDAPGDDG
                     MSNKVLEMAAAKYPGKPLRYVVLTHHHIDHTGGVRALSAAGATIVVGKGDGAFFRKML
                     SAPAGLNLYPIKGKVEPKVIEADGKWSVNDGGREIDAFALQTSHATPYLIPYIPDAKL
                     GWVTDLWNPGAPVTMSNPNLVDLVNGVKKMGIQPERFAGGHGAVGNYADVVKAVGG"
     gene            6679..8307
                     /locus_tag="VGM72_10330"
     CDS             6679..8307
                     /locus_tag="VGM72_10330"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4265705.1"
                     /translation="MIAKGITAALLVGAAGFSIALSLGYGVAAPAGGPQERVDAAYAA
                     MGGDRLAALKTISLKAHLAQYDPGESYSLHDPDKPGVNSSELIQYRDLARGLTRNEWW
                     DRPKNDDGTRRNYFEVVTPAAGWNVGNEASNGRTPKRAIMVNGVTAHTFSGKRLTVTL
                     RELERLDIVPAMKAHPERVTAMADQTVNGKTYPAVQYKSNVGTFIVMFDPVTNLPARV
                     RTPDWDALEGDSLYDAEFFDWRDVAGTKMPFHIFYTLNGMTICDMTYRDVVANPQMPA
                     NAFAIPPDQLAKAAKPADPRITPFHWVIRRTYSSFYMDVDNQYGDEGTKPTIADIGPY
                     ISETQGWTHNTVYIDMGPYLIAMEAPNDDGQAIAHLALARQKYGNKPIKYLVLSHHHV
                     DHVGGMRTFIAEGATVVVGKGDGDYFRKVLARPQTQNWNAPAKDKPYKPVVIENTGTW
                     TVKEGAREVDFYMIDNPHADDMQMGWLPDLKLGLVTDIWNPGPPVKASNPGLASVVHA
                     VDKLGLKPVKFAGGHGGVGDYAQAEKVVKDAEKK"
     gene            8402..9982
                     /locus_tag="VGM72_10335"
     CDS             8402..9982
                     /locus_tag="VGM72_10335"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HEY4265706.1"
                     /translation="MFRAKALRMDTPIPDQGALLVERVKTFPQADRRELLKYVAELTV
                     VGLVYFAVAKLGLTLASIHPSATPIWPTTGVTLAAVLLRGYRISPGIFAGALLANVTT
                     AGSIFTSLGIATGNTLESLVGAYVVNQWSDGLNTFDTPVGVGKFALTCLVPSTMISAT
                     LGVGSLSLAGYARLAEFRAIWTTWWIGDLAGALVITPVIVLWSTRIHPSLERNIPART
                     VALYTSAIAIGLVAFSPLFPGIPGRPPLAFLAVLPLMWAAVRCDQRDTATTAFLLSCF
                     AVWGAMANGGPFVRGNLNDSFLLLLAFMISVSVPSLALRAEVAVRKRHEQHVDFVMHE
                     LSHRSKNLLSVVQSMAHQVARRSDNFEAFFLGFSTRLRAFSETHDLLVRGNWHGAGIR
                     ELTQIQLAPFHNPDEDSIIAEGVDLTLTPKAAEQIGMALHELGTNAAKHGALSTATGM
                     VRIRWELETDEAQNPRLRLTWKETGGPRVRPSQREGFGTLMITKAVPATLQGTASLTF
                     EPQGVRWVLLTPSTNALI"
     gene            complement(10003..11709)
                     /locus_tag="VGM72_10340"
     CDS             complement(10003..11709)
                     /locus_tag="VGM72_10340"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY4265707.1"
                     /translation="MAAVFWAVSNPSAEAKAASGTAPASSRASFSVVEASIPQMRMAL
                     ARGRVTSRELVRQYLDRIAAYEDKLHAALTVNPRALEEADVLDRERRQGHIRGPLHGI
                     PIAVKDNIQTTDMPTTGGALAFDGLVPPYEATLVKNLRAAGAIIIAKTQMTELANWVA
                     GRPTPMPGNYNALGGYGYNPYDPRRDPREGFFDGRPAMLTGGSSSGIGTTANFWAANV
                     GTETSGSILNPSNWTMLAGIKPTVGRISRYGVIPITADQDTPGPMAKNVTDAAILLGV
                     LEGKAADPNDSATANCARPPRNDYTRYLKAGALKGARIGIPRAFFYEPATVPGEVLAQ
                     GGLNPDQMQAMEDAIAVLRKKGAVIVDPADIPSVVDPDPGNNFLNFGICGGGFNTGED
                     GSCSIVLEYGMKRDFNAWLQSLGARAPVKTLSELRDWNNAHAGAGAIKYGQSALDASD
                     ALDLARDYERYEADRAKDIRLTATHGIDEVMKRERLDALLFPAQTGAALAARPGYPTV
                     IVPFAKVPNAPEQPFPDGFDAKPAPFGVSFTGMACSEPQLIGLAFAFEQATKGRVPPS
                     LP"
     gene            11893..13056
                     /locus_tag="VGM72_10345"
     CDS             11893..13056
                     /locus_tag="VGM72_10345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HEY4265708.1"
                     /translation="MALSETAQSDARAWYRAERQNSVLRLILGGEWVLEESAPLDVSL
                     RQLHLQGENEVEIDGTGITRMDSAGAWLLIRTEHELAEQGARIRTLGIPQTYRPLLAM
                     LEREPTMHPPASHPDQSFTDLLFRIGRGTFNLLADGYQLLGFLGRVSVEAFEALVRPD
                     RELPWPAFVNQIEQTGVTAIPIVGLLSFLIGVVLAYQGSEQLQRFGAEIFTVNLVGIA
                     VFREIGGLMTAIIVAGRSGSAFTAQIGTMKLNQEVDAMQTIGVNPIETLVLPRVLGLL
                     VTLPLLTFYANIMGIIGGGVMCYFDLGITIPAFIRQLHDALIFSPWSFWLGLIKAPVF
                     AFIIALVGCFEGLRVEGNAGSVGKLTTRSVVESVFLVIVLDAGFSILFSLLGI"
     gene            13060..13857
                     /locus_tag="VGM72_10350"
     CDS             13060..13857
                     /locus_tag="VGM72_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4265709.1"
                     /translation="MVMRTKPRRAAPSKEEVVIRVRNLVNVLGGNLVHDHLDLDVKRG
                     EVLGVVGGSGSGKSVLLRTIVGLQKPTEGEICVLGQQIADLQGRELRALQMRWGVLFQ
                     EGALFSSQTVAENIQVPLREYFHLPQELMDEIAAMKLALVGLPADAGLKYPSELSGGM
                     KKRAGLARALALDPELLFLDEPTAGLDPISATQFDDLVHDLQKSLGLTVFMVTHDLDT
                     LKATTDRIAVLVNKTMKIGTIETLARDPDPWIHEYFTGVRGRAALSA"
     gene            13876..14808
                     /locus_tag="VGM72_10355"
     CDS             13876..14808
                     /locus_tag="VGM72_10355"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY4265710.1"
                     /translation="METRANYVLVGAFVLICVMGLFIATLWVAGSQYREEYTYYHTYF
                     DGAVTGLGKGTVVRYNGIDVGHVTDLAFDQADPKRVIVTLQIDPTLLLHSDSVASIES
                     QGLTGATYVEITGGTATSPLLRPGAGQEYAVIPSRPSTLQQLAQAGPELLAKLNVTGE
                     RFNALLSDDNRKALTETLQHLRAATALIDDHAEDLDKTLTNMKSATDALNRTLNSADR
                     AIASADRALGNVDSAVGSLQTASDTATVTVQKVGRLSDDADKVVNGQATAQFTQLMAQ
                     ARALVASLTRLTNDLEQQPTKLLFGDQRQGYTPK"
     gene            14805..15449
                     /locus_tag="VGM72_10360"
     CDS             14805..15449
                     /locus_tag="VGM72_10360"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEY4265711.1"
                     /translation="MSKAAGTSFNRRTMILGAGASLLSGCGSIQLIPQPMQPQLYVLR
                     PAIPAAMGARVPWRLSVAPADAPASLDTARIALSRSTNTMDYFANAAWNDRVPLIVQR
                     LLIEAFDASNRIVAVDRDSAGTENDYLLQTEIRDFEARYAMPDGAPEIVISIEAKLTR
                     TTRREIVNSLNVMQRAQAPANNLDSIVSAFNSAAGAAVAQIAAWTLDAPAPPAG"
     gene            complement(15464..16606)
                     /locus_tag="VGM72_10365"
     CDS             complement(15464..16606)
                     /locus_tag="VGM72_10365"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY4265712.1"
                     /translation="MRNCVRLPLAETWLARGQEPIAATQIGNMIYTSGVPGIDLKTGQ
                     PEEGPERQFARAFDNLMGLLQKLGAGPDSIGLLTVWIPGRDHRAFINKDWLRVFPNSN
                     RPARKTNQAPLPAGLDVQLMAACVLGESRVPVEIPGLSHKDPLPMGARMGPMVFSSVI
                     APEDPPNGKLVAGALPQIDRAFENMKLFMRSAGGSDSEINHAWVFMRDFTFQPPMVER
                     WVKDYPQFGNRPARKTLPYDLAGDTQIQVQITGHLGAARKNYEIPGVGHDDPIPMGSS
                     IGPLLQSSGMFGIDPATGKRVEGLQPQLTHALNNIRGLLDQADMTPEHVTHLTVMLQD
                     YADAPRVLDALRTLFPDSESGPALKFITYRMPAHWRVQFHVTAFRD"
     gene            16723..17316
                     /locus_tag="VGM72_10370"
     CDS             16723..17316
                     /locus_tag="VGM72_10370"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEY4265713.1"
                     /translation="MCMQEVELGGQKSFIGAWYIPDLKICDELIEFFQRSEAKAPGEI
                     GHNREINRGIKDSLDLIVTPEQFSTPVIRKYIINLYEVVKQYTDRYPYARLVNAWGIT
                     ENINLQYYRPGAGYKAWHTERIGKMLPSANRHLVFMTYLNDVSDAGGTEFLYQKIIAQ
                     PRKGLTLIWPVDWTHLHRGIVSPTEEKYIITGWFDFA"
     gene            complement(17339..17779)
                     /locus_tag="VGM72_10375"
     CDS             complement(17339..17779)
                     /locus_tag="VGM72_10375"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY4265714.1"
                     /translation="MLHIESLGLAEAEKALAGGIAAARAIGKAMAFAVADHAGEMIAT
                     ARMDGANPRILRHSVRKAYTSALMCRHTLSFKRDLKERDGALDQWGDVKLTTLPGGLA
                     VQRDGKFVGAVGAGGGGPDDERIARVMMAAMGFDIVEDARGGGR"
     gene            17911..18474
                     /locus_tag="VGM72_10380"
     CDS             17911..18474
                     /locus_tag="VGM72_10380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY4265715.1"
                     /translation="MSTQRDIVILVGSLRKESLSRKTANALAELAPTTLKLAIVEIGQ
                     LPHYNQDRETAAPPAEWAQFRDRIRPADGVIFVTPEYNRSVPGALKNAIDVGSRPSGK
                     SAFSGKPTGIVSVSPGAIGAFGANHHLRQSLVFLNMPAMQQPEAYIGGADKLFDESGK
                     LTNSSTREFLGKYMQAYAIWLERNATG"
     gene            complement(18524..>18865)
                     /locus_tag="VGM72_10385"
     CDS             complement(18524..>18865)
                     /locus_tag="VGM72_10385"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY4265716.1"
                     /translation="GCTIEAHNFADAMDDFEKLGATVIGVSHDSIATLDKFSLTECRS
                     KFPVAADTNGSVMNSYDAVSGGNPEYAQRTSYVISPDGRVLYTYTAGDPTHHVEYTMA
                     AVRKYVAEHKR"
BASE COUNT         3759 a         5838 c         5643 g         3625 t
ORIGIN      
        1 caggatttga tccgaagcga tggccgagcc tccgggcgaa tccacgcgca acaggatcgc
       61 cttcacattc ttgtcggcgg ccgcgtcgcg aatggcttgg gaaaaaatgt cgcccgcgac
      121 cgaattcccg gcgctgctca gcgggctgtc atcatcgccc tccacgatgt cgcccgcggc
      181 atggacgagc gcgatggtgg atgcgccgtc agatcccgca ttgcggttgc gcgtcgcctg
      241 cgcgtagcgc tcgaagcgca tgatcgcggc gcccgccccc gcccgcgcct tcgccgcgtc
      301 ccgcgcgtcg tcgtcataac cgatattggt gatgaggccc ttgtccttca ccgtctcgac
      361 ggtcgccggg ctcgcatcca gaaccgagat gagcgcggcg cggtcgagct tacggtcggc
      421 cgcggcttcc gccgttgccg aatcgtacca ggattgcagc acccgcatgg tggcttcgcg
      481 atgggccggc gtgaaatcgg tttcggtgaa gacattcgcg gcgttcttat attcatagcg
      541 ctgcacgaat tgcggcgtcg cctggacctt gtcgaatagc cccttgaaga acatcgtggt
      601 cgaggccgcg ccggcactga aaaaggtgct gaccggctgc atccagatct gatcggcggc
      661 agcggcggcg gtgtagtcgc cgagcccgct cgaatagaaa ctctgcgaat gggcgatgac
      721 aaatttcttg gattcctcga agtgcttcaa ggcatcgcgc agttcctcgg ctttgggcac
      781 cgagagatcg cctgatccca cgcgcaggaa aacgcctttg acgcgggaat cgcgcgccgc
      841 ccggtcaagc cccatcacca catccatgat cgagagcttg ccgttgagat cgaaaaggct
      901 tgaggtggat ttgtcgtcca tcgcgtttct gagatcgagt tccagaaccg cgtttcgcgg
      961 gacgccatcg ccgctgaaca tcgcaacgcc cgcgagcacc agcacgagga tgatcgccgc
     1021 caccgccgct ttggcgatgc cgttgagcac gctcttcacg attccccaca tccaggacag
     1081 aacgaccatg gcaaatccct ttccgacggc tgagcgtcgg actctatagg aaaaggctcg
     1141 gccaaggcca accgaaggcg tgcacaaaat cccatgccgc gacgggctca gcgagggttc
     1201 ggataaacac cctcacccct gcggctgtca ccgcatcctg attctgtcaa aagatgaccg
     1261 ctcgttccta cagcgcctgg gaaccgattt cgccggtgcg gatgcgcgtc acggagacca
     1321 ggtcgaacac gaaaatcttg ccatcgccga tcttgccggt gcgcgccgtg cgcatgatcg
     1381 tctcggtgac ctcgtcggcc ttctcgtcat cgacggcgat ttcgagcttc agcttcggca
     1441 cgtaattgat ggaatattcc gcgccgcgat aaacttcctt atggcctcgc tggcgcccat
     1501 agccccgcac ctcgctgacc gtcaggccgc tgatgcccga atcgttcaac gcctcgcgca
     1561 cggtatcgag acggtaaggc tggatgattg cgacgatgaa tttcattggc tggttcctcc
     1621 gaacgcaaca ttataccctg tctcgccatg gctggtgaga tcgaggccct gggtctcggc
     1681 atcgcggccc acgcgcaagc cgaccagtgc ttgggtgatc ttgacgagga tgaggcttgc
     1741 cacgcccgac catgcggccg ttgccgcgac cccgagcgcc tggacaccga attgcgagcc
     1801 catggtcaca ccctcgccat agccgacgcc gccaagcgcc actggcatga ggatggcggt
     1861 gagcaaggtt ccggtcatac cgccgacgcc atgcacggcg aaaacgtcga gcgcgtcatc
     1921 caccgtgaac cgcgttttca cgagatgaac cgtacaatag caaattgtgg agctgacgac
     1981 gccgatgaca agagcgccga acggtcccac aaaacccgaa gccggcgtga tggtcgccaa
     2041 tcccgcgacg gtgcccgtga ccgttccgac aagactcggt ttgccgaagc gggtccattc
     2101 gatcgccatc caggtgagag aggcaacggc ggcggccatg tgcgtcacca gcatcgccat
     2161 gccggcatcc gcgccggcgg tcagagcgga gccggcgttg aaaccaaacc agccgaccca
     2221 cagcatggcc gccccgacca tcacgatcca gggcgcgtgc ggcggctgca attgatgagg
     2281 aaatccgtca cgcttgccga gcgcggccgc aatcaccagc gcggaaatcc ccgccgtcgt
     2341 gtgaaccacg atgccgcctg cgaaatcctt gacgcccatg gccgcgagcc aaccgccccc
     2401 ccacacccaa tgcgccaccg gcacatagac gaccatcagc cacagtccgg agaacaacag
     2461 tacggcaccg aattttatgc gctcgacata ggccccgatg atgaggcccg gcgtgatgat
     2521 ggcaaaggtc atctggtaca taatgtagac gctgtcgggg atcgtcgtcc cggcatggac
     2581 cgcgttacgt cccaccccga tcaggaaggc ctggctgaga tcgccgatcc acgcattgcc
     2641 acccacgaaa accagcgaat aggcgcccgc gtaccacaga acggagataa gagaggcaac
     2701 cgccacgcaa tgcatgagaa ccgacaggac gttcttggcg cgcaccagac ctgcatagaa
     2761 aagcgccaaa cctggtaatg tcatgaacag aaccaggacg gttgacgtca acatccaagc
     2821 ggtatctgca ccattaatca tcaatttgcc cccatttatg ccttagcgga ttccgctaac
     2881 gcggcgttcc gctcttcttg taggcactct gctaattttt ccagctaagg caaagcggct
     2941 tatccgcctt cgacatgggt ggcgaatggc ggaagtcttg ccagcaaaac ccaattcccg
     3001 tgtccgagga tagcccgcag caccgtaaca tcctgaaaaa caagacatct ttgtcacatg
     3061 cgtttttgtg gacaaaagct tacggacgtc ctcgcgcagg tgccaaatcc tgttaagttt
     3121 cccccaaaga aaaatgggga ggcaattcga tgcgtcgcga aaatcgaacg atctcggtca
     3181 tgaccgttct gggcgtgggt gtggctgtgg ctttgagcgg ccaatccgcc cgaagcgctc
     3241 cggccacgac gcctcaggcg cgcgccgagg ctgccttcca ggccatgggc ggcgacaagc
     3301 aggagatggt caccagcatc gtcctcaaag gaaccatggc gcagtgggaa ccgggcgaat
     3361 ccttttccgt cgccgacctg accatgcccg atggcggcac ttcgaccttc accgaaacgc
     3421 tggaccgtac caagggctac cagcgcatcg aatgggtgag gccgcgtgcc gccggcggca
     3481 cgcgcagcta tgccgagatc atcatgcccg acggtggcta cgtgatcggc aatgacgcca
     3541 atggcgcccc gcccaagcgc gatcaacacg cccgccaatc tgccggcgca tacgatgtcc
     3601 gccatccggc tcaccgccac gctgcgtgag atcgaacgca atgacatcgt gctggcgatg
     3661 catgagcatc ccgagcttat ctccgaagca cccgcacaaa cgatcggcgg caaatcctat
     3721 ccggcggttc agttccgcgg cgattacggc agctttaccg tcgctttcga tccggacacc
     3781 catttgcccg ccatggtgcg tacccgtgat ttcgatcagc tcatgggcga cgccgatttc
     3841 gacgaaagtt tttcggattg gcgcgatgtg gacggcctca agatcgccca ccggatgact
     3901 tatacgctca acggcatgaa aatcttcgac accacgctga acgatgcgcg ggtcgatatt
     3961 gtgggaaatt ccatccttcc ggccgacacg ttcgcggtcc cgaccgcaat ccgcggcaag
     4021 gcgcccaaac cctcgtccgt ggacaagccg ccctatcaat ggatccttcg gcggctggcg
     4081 tccggatttt tcctggactc agacaatctc tacacggacg atggcggcag cctgacgctg
     4141 agcgatgtcg cgcccaatat cagcctcgtg acggggggaa cgcacaacac cctgatcgtc
     4201 gccacgaaca cctatctcgt ggctttcgat tcgccgggcg acgacggcca atcgaaactg
     4261 gcgatcgatc tggccaagca gaaatattcg ggcaagccgt ttcgctatct tgtgctgacc
     4321 catcatcata tcgaccactc aggcggcttg agggcctata tcgccgaggg cgcgacattg
     4381 gtggtcggca agggcgatgg agccttcttc cgcaaggtgt tgatggcgcc ccaaacactg
     4441 aacgtgaata agcccgcggg cgcggtgacg cccaaaatca tcgaagtcgc tggcaagtgg
     4501 agtgtgaaag acggcggacg cgagatcgac gcctacgtca tcgataatcc gcacgcggcc
     4561 gactatctga tgccttatgt ccccgacgcg aaactcggct tcatcaccga cctgtggaat
     4621 cccggacctc ccgtcgcggc cgcgaacccg aatttgatcg ccgtggtgaa aggcgtggaa
     4681 aaatggatgt tgatgccgga gcgctttgcg ggcgggcatg gggcggtggg aaattacccg
     4741 gatgtaatgc agggggccgc cggagcaaat acgcgctagc gggtcgaatc cctcctggca
     4801 gaacggcggg cgcgttccta taatggcgcg gatgggcgga ctttttcggg gtctgccccc
     4861 gcaaaagctg gagggaggca attcatgctg aaaaaaagca cgattattac cgcgctgttt
     4921 ggcgccgcag gcatctctgt tgtgctgggg ttgggcaccg tggcgtgggg tcaggctccc
     4981 gggacgccgg gcagcggcga tgcgggggct ggcgtgggcg tgctctccac cgggaagggg
     5041 ccccacgcgc tcgccgacgc ggcctacaaa gccatgggcc tgaccgacga gaagcttgct
     5101 cccgccgtcg tcacgctcat cgtcaagggc actttgcagg cctgggatcc gggcgaatcc
     5161 gaatcgctca gcgaccccta caaacccgat tggggcacgt cgacctatac cgaatctttt
     5221 gaccgcgccc gggggctcta ccgcatcgaa tggatgcgtc cgagggccgg tggcggaatg
     5281 cgcaattaca ccgaaatcct caccgacgaa tacagtccca gcggcgagca gatgggcggc
     5341 tacgtcatgg gcgtcgacgt caatggcggc cagccgatgc gcgccgtgat ggtcaatgga
     5401 atgcccgcgc accagatgtc gggaaagcgg ttgaccgtaa cattgcgcga actggaacgc
     5461 gacgccgtgc tgggcgacat gcatgagcat ccggagcgcg tttcggaaat cgccgaccag
     5521 cgcgccggag gcaagaccta caaggcggcc cagtatcgcg ccgatcacgg cacctttatc
     5581 gtattgttcg atccggcgag ccatcttccg gccgtggtcc gcacccgcga ttgggacgtg
     5641 tactatggcg attccgatta tgacgagacg ctctcggact ggcgcgatgt cggaatgggc
     5701 atcaaactgc cattccataa gctcattacg atcaatggga tcaagatctt cgacgagaag
     5761 ctggacaacg tccggctgaa tccgcagatc gcggtggatg ccttcaccgt tcccgccgtc
     5821 cttcgccgcg gcgcgcctgc cgccgctccc acggggcagg tgccttatca gtggattctc
     5881 cggcgcatgg gcaacgggtt ctacctcgat tccgatgctc tctacacgga tgatggcggc
     5941 agcctgaagc tggtggacat cgcgccgaac gtcagcatgg tgcagggcgg ttcgcacaac
     6001 acgctcatcg tcgccaccaa cacctatctc gtcgccttcg acgcccccgg cgacgacggc
     6061 atgtccaaca aggtgctgga gatggcggct gccaaatatc ccggcaaacc gttgcgctat
     6121 gtcgttctca cccatcatca catcgaccac accggcggcg tgcgggcgct gtccgcggca
     6181 ggcgccacca ttgtcgtggg caaaggcgac ggcgctttct tccgcaagat gctgtccgcg
     6241 ccggccggcc tgaacctcta tcccatcaag ggcaaggttg agcccaaagt gatcgaggcc
     6301 gacggcaaat ggagcgtgaa cgacggcggc cgcgagatcg acgcctttgc gcttcagacc
     6361 tcacatgcga ccccctacct gatcccgtat attcccgatg cgaagcttgg ttgggtcacc
     6421 gatctgtgga atcccggcgc acccgtcacg atgtcgaatc ccaatctggt cgatctcgtc
     6481 aacggcgtga aaaaaatggg gattcagccg gagcgtttcg ccggtggtca tggcgcggtc
     6541 ggcaattatg ccgacgtggt caaggcggtg ggcggctagc agggtttcaa tccatctggc
     6601 agaacagact gacaatcctg taatgttgcg aaagggcgag ctctcgcgag ctcgcccttg
     6661 gcttaggagg gaacatccat gatcgccaaa ggcataacgg ccgctctgct ggtgggtgcg
     6721 gcggggttca gtatcgcgct cagcctggga tatggcgttg ccgcgcccgc gggcgggccg
     6781 caggaacggg tggacgcggc ctatgccgca atgggcggcg acaggcttgc cgcgctgaag
     6841 accatcagcc tgaaagccca cctcgcccaa tatgatcccg gcgaatccta ttccctccac
     6901 gatcccgaca aaccgggcgt caattcctcg gagctgatcc aatatcgcga tctggcgcgc
     6961 gggctcacgc gcaacgaatg gtgggaccgc cccaagaacg acgacggcac gcggcgcaat
     7021 tatttcgagg tcgtgacgcc ggccgccggc tggaatgtcg gcaatgaggc ctccaacggc
     7081 cgcacgccca agcgcgccat catggtcaat ggcgtgacgg cccacacgtt ttcgggcaag
     7141 cgcctcacgg tgacgctgcg cgaactcgaa cgcctcgaca tcgtgccggc gatgaaggcc
     7201 catcccgagc gcgtcacggc gatggccgat cagacggtga acgggaagac ctatccggcg
     7261 gtgcaatata aaagcaatgt cggcacgttc atcgtgatgt tcgatcccgt cacgaatctg
     7321 cccgcgcgcg tgcgcacacc cgattgggat gcgctcgaag gcgattcgct gtatgacgcc
     7381 gaattcttcg actggcgcga tgtcgccggg acgaaaatgc cgttccacat attctacacg
     7441 ctcaacggaa tgacgatctg cgatatgacc tatcgcgacg tcgtcgccaa tccgcagatg
     7501 ccggcaaatg ccttcgccat tccgccggac cagctcgcca aggccgccaa gccggccgat
     7561 ccgcgcatca cgccgtttca ttgggtgatc cggcggacct attcgtcatt ctacatggat
     7621 gtcgacaatc aatatggcga tgaaggcacc aagcccacca tcgccgatat cggaccctat
     7681 atcagcgaga cccagggttg gacccacaac accgtctaca tcgacatggg cccttatctt
     7741 atcgcgatgg aagcgcccaa tgacgacggc caggcgatcg cccatctggc gctggcgcgg
     7801 cagaaatacg gcaacaagcc gatcaaatat ctcgtgctca gccaccacca tgtcgatcat
     7861 gtcggaggca tgcgcacatt catcgccgag ggcgcgaccg tcgtcgtcgg caagggcgac
     7921 ggcgattatt tccgcaaagt gctcgcgcgc ccgcaaaccc agaattggaa cgcgcccgca
     7981 aaagacaagc cctacaaacc ggtggtgatc gagaacaccg gcacctggac cgtgaaggaa
     8041 ggcgcgcgcg aggtcgattt ctacatgatc gacaatccgc atgcggatga tatgcagatg
     8101 ggctggctgc ccgatctgaa gctcggcctc gtcaccgaca tctggaatcc cggaccgccg
     8161 gtaaaggcat ccaatcccgg tcttgcctcg gtcgtccacg cggtggacaa attgggattg
     8221 aagccggtga agttcgcggg cggccatggc ggcgtcggcg actatgccca ggcggaaaaa
     8281 gtggtgaagg acgcggaaaa gaaatagtcc ctacccgccc ccaaaaaggc ccacgcgata
     8341 atgctttatt aaccaacatt aatacaggat aaatcgaggc tacccacatc gaaccggccg
     8401 catgttccga gcaaaggccc tgcgcatgga cacacccatt ccagaccagg gggctctgct
     8461 cgtcgagcgt gtcaaaacat ttccgcaggc cgatcgtcgc gaactgctga aatacgtggc
     8521 cgaattgacg gtcgtcgggt tggtctattt tgcggtggcc aagctcggtt tgacgctggc
     8581 atcgatccat ccgagcgcaa ctccgatctg gccgacaacg ggcgtcacgc tggcagcggt
     8641 gttgttgcgt ggctaccgga tttctccggg tatattcgcc ggagcgctgc ttgccaatgt
     8701 aacgaccgca ggctcgatct tcacatcgct gggaattgcg accggcaaca cgttggaaag
     8761 ccttgtcggc gcatacgtcg tcaatcaatg gtctgacggg ctgaacacat tcgacacgcc
     8821 tgtcggagtt ggcaagtttg cgctgacctg tctcgtgcca agcacgatga tcagtgccac
     8881 cttgggtgtc ggcagcttga gtttggccgg ctatgcgcgg ttggcggaat ttcgcgcgat
     8941 atggacgaca tggtggatcg gcgacctggc gggagcgctc gtcatcacac ccgtcatcgt
     9001 gctgtggagc acgagaatcc atccctctct cgaacgaaac attccggccc gcacggtcgc
     9061 gctctacaca tcggcaattg ccatcggact ggttgccttc agtccgctct tccccggaat
     9121 tcccggcaga ccgcctttgg cgttcttggc ggtgttgccg ctgatgtggg cggccgtgcg
     9181 ttgcgatcaa cgcgataccg ccaccacggc ctttcttttg tcctgttttg cggtttgggg
     9241 cgcgatggca aatggcgggc cgttcgtccg cggtaacctc aacgattcct tccttttgct
     9301 gctcgccttc atgatcagcg tttccgttcc gagcctggct ttgagggctg aagtggcggt
     9361 gcgcaaacgg catgaacagc acgtcgattt cgtcatgcac gagctatcgc accgatcgaa
     9421 aaatctcctt tccgtcgtgc agagcatggc gcatcaggtt gcgcgccggt ccgataattt
     9481 cgaggcgttt ttcctgggtt tcagcacgcg tctgcgcgcc ttttcggaaa cccacgatct
     9541 tctcgtccgc ggcaactggc acggtgccgg cattcgggaa ctgacgcaaa ttcagttggc
     9601 gccgttccac aatccggatg aagattccat tattgccgaa ggggtggatc tcacactgac
     9661 tcccaaagcg gccgaacaga tcggcatggc gctccacgaa cttggaacga atgcagcaaa
     9721 gcatggcgcg ctttccacgg cgaccgggat ggtgcgcatc cggtgggaac tcgaaaccga
     9781 tgaagcacag aatccgcgtt tgcgattgac ctggaaggag accggcggac cgcgtgtcag
     9841 gccgtctcag cgagaggggt ttgggactct catgatcacc aaagccgttc cggcgacatt
     9901 gcaaggtacg gcctctctga cgtttgagcc ccaaggagtc aggtgggtgc ttctgacacc
     9961 ctccacaaat gccctgatct gattcaagag gtctatgcgc cgttacggca aggatggggg
    10021 cacgcggccc ttggtcgcct gctcgaacgc aaaagccagc ccgatcaatt ggggctcgct
    10081 gcaggccatg ccggtgaaac tcaccccaaa cggcgcgggc ttcgcatcga agccgtccgg
    10141 aaatggctgt tccggcgcgt tcggcacctt cgcgaagggc acaatcaccg tggggtagcc
    10201 gggacgcgcc gcgagcgcgg caccggtctg cgcggggaat agcagcgcgt ccagccgctc
    10261 gcgcttcatc acctcatcga tgccgtgcgt tgcggtgagc cggatgtcct tggcgcggtc
    10321 ggcctcgtag cgctcgtaat cgcgcgcgag gtcgagcgcg tcggaggcat cgagcgccga
    10381 ttggccgtat ttgatcgcgc cggcgccggc atgcgcgttg ttccagtcgc gcaattcgct
    10441 cagcgtcttc accggcgcgc gcgcacccag cgattgaagc caggcgttga agtcgcgctt
    10501 catgccgtat tccaggacaa tggagcagct tccgtcctcg cccgtgttga agcctccccc
    10561 gcagatcccg aaattcagga aattgttgcc gggatcgggg tcgaccacgc tcgggatatc
    10621 ggccggatcg acgatcaccg cgcccttctt gcgcagtacg gcgatcgcat cttccatcgc
    10681 ctgcatctgg tccggattga ggccgccttg cgcgagaact tcgcccggca ccgtggctgg
    10741 ctcataaaaa aacgcgcgcg gaatgccgat gcgggcaccc ttcagcgcgc cggctttcag
    10801 atatcgggtg taatcgttac ggggcggcct tgcgcaattg gccgtcgcgg aatcgttcgg
    10861 atcggccgct ttgccttcga gcacgcccaa caggatggcc gcatcggtga cgtttttcgc
    10921 catcggacca ggtgtgtcct gatcggcggt gatggggatg acgccgtaac ggctgatgcg
    10981 tcccaccgtg ggcttgatgc cggcgagcat ggtccaattg gacggattga ggatcgagcc
    11041 cgaggtttcc gttcccacat tcgcggccca gaaattggct gtcgtaccga tgccggaact
    11101 tgacccgccc gtgagcatcg ccggacggcc atcgaagaaa ccctcacgcg gatcgcggcg
    11161 cggatcgtag ggattatagc catagccgcc gagcgcattg tagttgccgg gcatcggagt
    11221 cggccgcccg gcgacccaat tggcgagttc cgtcatctgg gtcttggcga tgatgatcgc
    11281 tcccgccgcg cgcaaattct tgaccagagt cgcctcatag ggcggcacca gaccgtcaaa
    11341 agcgagcgcg ccgccggtcg tgggcatatc cgtggtctgg atgttgtcct tcaccgcgat
    11401 cgggatgcca tggagagggc cacggatgtg tccttgcctg cgctcgcggt cgagcacatc
    11461 ggcctcctcc agagcgcggg gattgacggt gagcgcggca tggagtttgt cctcgtaggc
    11521 cgcgatgcgg tcgagatatt gccggaccaa ttcgcgggag gtcacgcggc cccgcgccag
    11581 cgccatccgc atctggggaa tgcttgcctc cacgacgctg aagctcgcgc ggctggacgc
    11641 cggtgcggtc ccggaggcag ccttggcctc ggcggagggg ttggaaacgg cccaaaagac
    11701 cgccgccaat aaacagcttc tcagtcgcat cggtcgttgt tcttttcggg tgcagcagcc
    11761 caatgtaccg gcgcgtctgg cgccggccaa gctttgccct gcgattttgc gtgtgcagcg
    11821 atttggccga tttgacgttg acttggctgg gcctcacaaa tggggctcgc ctgcgaaatc
    11881 gccccaaagg gaatggctct gagcgaaacc gcccaatccg acgcccgcgc ctggtacagg
    11941 gccgagcggc agaattccgt cctgcgcctt atccttggcg gcgagtgggt ccttgaggaa
    12001 tccgcgccgc tcgatgtctc gttgcggcag cttcatcttc agggcgagaa cgaggtggag
    12061 atcgacggca ctggcatcac ccgcatggac agcgcgggcg cctggcttct gatacgtacg
    12121 gagcacgaat tggcggagca aggtgcgagg attcgcacgc tcggtattcc ccagacctat
    12181 cgtcctttgc tcgccatgct ggagcgcgag ccaacgatgc acccacccgc atcgcatcct
    12241 gatcaatcct tcacggacct tttgttccgc atcggccggg gcacatttaa tttgctggcc
    12301 gatggatatc agcttctggg gtttctcgga cgcgtctcgg tggaggcgtt cgaagcattg
    12361 gtcaggcccg atcgcgaact tccctggccg gccttcgtca accagatcga gcaaaccggc
    12421 gtcaccgcca ttccgatcgt cggccttttg tcgttcctga tcggtgttgt cctcgcctat
    12481 caaggatccg aacagcttca gcgcttcggc gcggaaattt tcacggtcaa tctcgtcggg
    12541 atcgcggtct tccgtgaaat cggcggcctg atgacagcca tcatcgtcgc ggggcgctcg
    12601 ggatcggcgt tcaccgcgca gatcggcact atgaagctca atcaggaggt ggacgcgatg
    12661 cagacgatcg gcgtcaatcc gatcgaaacg ctggtgcttc cgcgcgtcct gggattgctg
    12721 gtcacgctgc cgctgctgac cttctacgcc aacatcatgg gcattatcgg cggcggagtg
    12781 atgtgttatt tcgatctcgg catcaccatt ccggctttca tacgccaatt gcacgacgcg
    12841 ttgatattca gcccgtggag cttttggctc ggcctcatca aagcgcctgt ctttgccttt
    12901 atcatcgcgc tggtgggttg cttcgagggt ttgcgcgtcg agggcaatgc cggcagcgtt
    12961 ggaaaactga ccacccgctc ggtggtggaa tcggtcttcc tcgtgatcgt gctggatgcc
    13021 ggtttctcga ttctgttttc gctcctgggg atctgagcga tggtgatgcg gaccaaaccg
    13081 agacgcgcgg ctccctccaa ggaggaggtg gtgatccgtg tgcgcaatct ggtgaatgtg
    13141 ctcggcggaa acctggtcca cgatcatctc gatctcgacg tgaagagggg cgaggttctg
    13201 ggtgtcgtcg gcggctcggg atcgggcaaa tccgtattgt tgcgcaccat cgtgggactt
    13261 cagaaaccca ccgaaggcga aatctgtgtt ttgggacagc agatcgccga tcttcagggg
    13321 cgcgagctga gagccttgca gatgcgctgg ggcgtccttt tccaggaagg cgcattattc
    13381 agctcccaga cggtcgcgga gaatatccag gttcccttgc gggaatattt ccatctgccg
    13441 caggagctga tggatgagat cgcagccatg aaattggcgc ttgtgggttt gcctgccgat
    13501 gcgggtctca aatatccttc cgaactttcc ggcggaatga aaaagcgcgc cggactcgcg
    13561 cgcgcgctcg cactcgatcc cgaattgctg tttctggacg aacccactgc tgggctcgat
    13621 ccgatttccg caacgcagtt cgatgatttg gtgcacgacc ttcagaaaag tctcggcctc
    13681 accgtgttca tggtcacaca cgatctcgac acgctgaagg ccacgaccga ccgcatcgcc
    13741 gtgctcgtga acaaaaccat gaagatcggc accatcgaga cgctggcgcg cgatcccgat
    13801 ccctggatcc acgaatattt cacaggcgtg cgcggacgtg ccgcgctcag cgcctgagtt
    13861 caaggagaaa ttcacatgga aacgcgcgca aattatgtat tggtcggcgc ttttgtgctg
    13921 atctgcgtta tgggactttt cattgcaacg ctgtgggtcg caggctcgca atatcgcgag
    13981 gaatacacct attaccacac ctatttcgac ggagccgtca ccggcttggg aaagggcacc
    14041 gtggtgcgct acaacggcat cgatgtcggt catgtcacgg acctcgcatt cgatcaggcc
    14101 gatccgaagc gggtgatcgt cacgttgcag atcgacccca cattactgct gcattcggat
    14161 tcggtggcct ctatcgaaag ccagggactg acgggcgcta cctatgtcga gatcaccggc
    14221 ggcaccgcga catcgccgct gctgaggccc ggggccggtc aggaatatgc cgtgatccca
    14281 tcgcgtcctt cgacattgca gcaattggcg caggccggcc cagaactgct ggcgaaattg
    14341 aatgtcaccg gcgagcggtt caacgctctt ctgagcgatg acaatcgcaa ggccctgacc
    14401 gaaacattgc agcatttgcg cgcggcgacc gcccttatcg acgatcacgc cgaagatctc
    14461 gacaagacgc tgaccaacat gaaatcggca acggacgcgc tcaatcgcac cctcaacagc
    14521 gccgaccgcg ccatcgccag cgcggatcgc gcattgggaa atgtcgattc ggcggttggc
    14581 tcgttgcaaa cagcttcgga taccgcaacg gtcacagtcc agaaggtcgg ccgcttgtcc
    14641 gacgatgccg acaaggtggt gaacggacag gcaaccgcgc aattcaccca actcatggcc
    14701 caggcacgcg cgctggtcgc cagcctgacg cgtctcacaa acgacctcga gcaacagccg
    14761 accaagcttc tcttcggcga tcaacgacag ggatacacac cgaaatgagc aaagcggcag
    14821 gcacgagttt caaccggcgg acgatgatcc tcggggccgg tgcgagcctg ctttcaggct
    14881 gcggctccat tcaattgatt ccacagccga tgcagccgca gctttatgtg cttcgccccg
    14941 ccattcccgc ggcgatgggt gcgcgcgtcc catggcggct ttcagtcgcg cccgccgatg
    15001 cgccggccag cctcgacacc gcgcgcatcg cgctttcgcg ctcgaccaac acgatggatt
    15061 atttcgccaa tgcggcctgg aacgaccgcg tgccgctgat cgtgcagaga ctgctgatcg
    15121 aggcgttcga cgccagcaac cgcatcgtgg cggtggatcg cgacagcgca ggaacagaaa
    15181 atgattacct gctccaaacg gagattcgcg attttgaagc gcgctacgca atgcccgacg
    15241 gcgcccccga gatcgtcatc tccatcgagg cgaagttgac gcgcacaacc cggcgcgaga
    15301 tcgtaaacag cctcaacgtc atgcaacggg cccaggcgcc ggcgaacaat ctggacagca
    15361 tcgtctcggc atttaattcc gcagccggtg cggcggtcgc gcagatcgcg gcctggacgc
    15421 tagatgcgcc cgctcctcct gcgggttagc gctgggtttc ccttcagtcg cggaatgccg
    15481 tgacgtgaaa ctgaacccgc caatgggccg gcatgcggta ggtgatgaat ttcagtgccg
    15541 gtccggattc cgaatccgga aaaagcgtgc gcaacgcgtc caacacgcgc ggcgcatccg
    15601 cataatcctg cagcatcacg gtcagatgcg tgacgtgctc cggcgtcatg tccgcctgat
    15661 cgagcagacc gcgtatattg ttgagtgcat gcgtgagttg cggttgcagg ccttcgacgc
    15721 gttttcccgt cgcgggatcg atcccgaaca tgccggagga ttgcagcagg gggccgatgc
    15781 tggatcccat cggtatcgga tcgtcatggc cgacgccggg aatttcgtag ttcttgcgtg
    15841 cagccccgag gtgccccgtg atctgcacct gaatctgcgt atcgcccgca agatcatagg
    15901 gtagcgtttt gcgcgccggc cgattcccga attggggata gtccttcacc cagcgctcga
    15961 ccatgggtgg ctggaacgtg aaatccctca tgaacaccca ggcgtgattg atttcggaat
    16021 ccgagcctcc ggcggagcgc atgaaaagct tcatgttctc gaaggcgcga tcgatctggg
    16081 gcagcgcgcc tgcgaccaat ttcccgttcg gcggatcttc cggcgcgatc acggacgaga
    16141 acaccatcgg ccccattctg gcccccatcg gcagcggatc cttgtgcgaa agccccggaa
    16201 tttcgacggg aacacgcgat tcgcccagca cgcaagcggc catcaattgc acgtccaatc
    16261 cggctggaag cggcgcctga ttggtcttcc gcgcgggccg gttgctgttc gggaaaacgc
    16321 gcagccaatc cttgttgata aaggcgcgat ggtcgcggcc gggaatccag acggtaagca
    16381 ggccgatcga atccggtccc gcgccgagtt tttgcagcaa gcccatcaga ttgtcgaaag
    16441 cgcgcgcgaa ttgccgttcg ggaccttctt ccggctggcc ggttttcaga tcgatgcccg
    16501 gaacaccgga cgtgtagatc atgttgccga tctgcgtcgc cgcgatcggt tcctgcccgc
    16561 gtgcaagcca ggtctccgca agcggcagac gaacgcaatt gcgcattgat acctcccgag
    16621 acgtttgtcg gctgctagat cgggctatgc cgatgcagta atcttttctc atatcgtgca
    16681 ggaatgtcag cttttttctg acaatgtttg ccgccggaga ccatgtgtat gcaggaagtc
    16741 gagcttggcg gccaaaaatc cttcataggg gcgtggtaca tccccgacct aaaaatctgc
    16801 gacgaattga tcgagttctt tcagcgcagc gaggcgaagg cgccgggaga gatcgggcac
    16861 aatcgcgaga tcaacagggg catcaaggat tccctcgatc tcatcgtcac gccggagcag
    16921 ttctccacgc cggtcatccg gaaatacatc atcaatctct atgaggtggt gaagcaatat
    16981 accgaccgct atccctatgc gcgcctcgtc aatgcgtggg gcatcaccga aaacatcaat
    17041 ctgcaatatt atcggccggg cgccgggtac aaggcttggc acaccgaacg catcggcaag
    17101 atgctgcctt cggcaaaccg gcatctggtt ttcatgacct atctgaacga tgtgtcagac
    17161 gcggggggca cggaattcct gtatcagaaa atcatcgccc agccccgaaa aggcctgacc
    17221 ctgatatggc cggtcgattg gacgcatctg catcgcggca tcgtttcccc aactgaggaa
    17281 aaatacatca tcaccggctg gttcgatttc gcctgaccgc cggaatgcgc gcggccattc
    17341 agcgaccacc gccacgcgcg tcctcgacaa tgtcgaaccc cattgctgcc atcatgacgc
    17401 gcgcgatccg ttcgtcatcc gggccgcccc cccctgcacc aacggcaccc acgaatttgc
    17461 cgtcgcgttg aaccgcaagc ccacccggca gagtcgtcag cttcacatcg ccccattgat
    17521 ccaaggctcc atcgcgctcc ttgagatcgc gtttgaagct gagcgtatgg cggcacatca
    17581 gggccgaggt gtaggccttg cgaaccgaat gtctgaggat tcgcggattg gcaccatcca
    17641 tgcgcgcggt ggcgatcatt tcgcccgcat gatcggcgac cgcaaaggcc atcgccttgc
    17701 cgattgcacg cgcagccgcg atgccgccgg caagggcctt ctccgcttct gcaagtccga
    17761 ggctttcgat atgcagcatg gggttctccg cacaaggaac gtgcggcgat aaagcagagg
    17821 cggcctgccc gttcaaggcc ggtatagttc ggcttcattt tccggctctc gcgttacctg
    17881 agaatcgaac ccggattgag agaggtctgc atgagcactc aacgcgatat cgtcatcctg
    17941 gtcggcagtt tgcgaaaaga atcattgagc cgaaaaaccg ccaacgctct tgccgagctt
    18001 gcgccaacga ccctcaaact cgccatcgtc gagatcgggc aattgccgca ttacaaccag
    18061 gatcgggaga ccgcggcgcc gcctgccgaa tgggcccagt ttcgggaccg cattcgcccg
    18121 gcggacggcg tgatattcgt gacgcccgaa tataatcgtt cggtgccggg cgcgctgaaa
    18181 aacgcgatcg atgtgggctc ccgtcccagc ggcaaatccg ctttcagcgg aaagcccacc
    18241 ggcatcgtca gcgtgtcacc gggtgcaatc ggcgcgtttg gcgccaacca tcatttgcgc
    18301 cagtcgctgg ttttcctgaa catgcccgca atgcagcagc ccgaagccta tatcggcggc
    18361 gccgacaagc tgtttgacga aagcggcaag ctgaccaaca gctctacccg cgaattcctg
    18421 ggcaaataca tgcaggccta tgcgatctgg ctcgaacgca acgcgactgg ctgaaacgaa
    18481 agcgagcctc gatggagaac ccgcgtctgg ggtgtatccg gcatcagcgc ttatgctcgg
    18541 cgacgtattt gcgcaccgcg gccatggtgt attcaacgtg atgggtcgga tcgcccgcag
    18601 tgtaggtgta gagcacgcgg ccatccggcg agatcacata ggaagtgcgc tgggcatatt
    18661 ccggattgcc tccagacacc gcgtcgtaac tattcatgac gcttccattg gtgtcggccg
    18721 cgaccgggaa tttgctgcgg cattcggtca gcgagaactt gtccagcgtg gcaatggaat
    18781 cgtgggaaac gccgatcacc gttgcaccaa gcttttcgaa atcgtccatc gcatcggcga
    18841 aattatgcgc ctcgatggtg cagcc
//
LOCUS       DASYEG010000240         5144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2550523, whole genome shotgun sequence.
ACCESSION   DASYEG010000240 DASYEG010000000
VERSION     DASYEG010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2550523"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VGM72_10390"
     CDS             complement(<1..764)
                     /locus_tag="VGM72_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4265717.1"
                     /translation="MAGPQYVYVMKGLSKTFPGGRQILKDIWLSFLPGAKIGIVGVNG
                     AGKSTLLRMMAGVDKEFNGEAWAADGVRVGYLPQEPELDPQLNVLGNVMEGVAEKKAL
                     LDRYNEIAANYTDETAEEMSSLQDKIDAQNLWDLDAQVEQAMDALRCPDPEADVNNLS
                     GGEKRRVALCKLLLEAPDMLLLDEPTNHLDAASVSWLERHLSDYAGTVVLVTHDRYFL
                     DDVTGWILELDRGRGIPHEGNYSSWLDMQEKRLAQE"
     gene            complement(848..1894)
                     /locus_tag="VGM72_10395"
     CDS             complement(848..1894)
                     /locus_tag="VGM72_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265718.1"
                     /translation="MRKRVATAALSVLAGALWVPLAYAAAPSAILGPLSSVSPDRNAH
                     AVNLNLGGLMTSGLAEDVPALRPNAIGIANGVEIQLSRGRNWDPYADLFPAASALSSA
                     YLTGTNTRASMSFVLGKGVTLDASHVALGLGALSDAQPSGLARDLAQRLGSDLQSVGT
                     TSANLNWNFSDWGGLGLTASHANGDGSLLSNIPASLRGAVSTDSSALGISARVGFGEG
                     WITTLTYAEGVTQLDLSRDRLVTSGGGDPVHSEAYGIGLAKKGLFGDDVFGIALSRPL
                     QIYTGSVGFGALNTNFALANTQTRESDVELGYVTTFLDGTLALQANAAYQLNAAGTRG
                     QNAVTGVARAKLNF"
     gene            complement(2101..3315)
                     /locus_tag="VGM72_10400"
     CDS             complement(2101..3315)
                     /locus_tag="VGM72_10400"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY4265719.1"
                     /translation="MYELLKGVRVIDLTTTYLGPYATQFLGDMGADVIKVESLDGDVG
                     RSPRPSRSDGMGAGFLNTNRNKRSIAIDLRAPEGRDVVLALLGGADALVHNMRSAAAA
                     KLGLAYEDAKRANARIVYCYSPGFGQNGPYAAEPAYDDIIQAMSGLAQLNADSTGAPR
                     FLPSIVADKVVGLHLAFAVATGLVHRLKTGEGCAIEAPMFESMVAFLLVEHLAGRSFV
                     PPLGPPGYERMLAKNRRPYKTRDGYIAIMPYTTQQWARFLECVGRGDLLAADWVKDPV
                     QRSANVDALYQMIADAAPGHSTAEWLVLLNERDIPCGPVNSLEALFEEPHLKDAGLFE
                     DFVHPAEGQMLGIRSPFRVADLPRQPDRPAPRLGEGSESILQEAGFRPETIRDLVARN
                     IVKPSAGVASKP"
     gene            complement(3315..4070)
                     /locus_tag="VGM72_10405"
     CDS             complement(3315..4070)
                     /locus_tag="VGM72_10405"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY4265720.1"
                     /translation="MAPAEILLLVTVGFLAGTCNALVGGGTLFTFPVLLAAGLPPVLA
                     NTTTTVALVPGTLTSAYAYLPELGRLREHLPKRIVAATAGGLLGAVLLLVSGNAVFFY
                     LVPWLLGAATALFTFSRRIVRRVTKDPHRKNETRLLGMEFVSAIYGGYFGAAIGILLL
                     ASMALAGERDIQSANAQRNFLVCFINGVAALIFIVTGSVAWNVALIVMAGAIGGGYFG
                     ARVARRIPNEALRVIITAAGALFSVYYFVKAYG"
     gene            complement(4115..>5144)
                     /locus_tag="VGM72_10410"
     CDS             complement(4115..>5144)
                     /locus_tag="VGM72_10410"
                     /inference="COORDINATES: protein motif:HMM:NF018017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mitochondrial fission ELM1 family protein"
                     /protein_id="HEY4265721.1"
                     /translation="HAGCSAITASSAGALSAWITCLGSEAPPLSCWVVTDGKAGMESQ
                     CVGLAEALGLTPVVKRVKLRWPWRELTPYLRVGGRAQFAGGSDILAPPWPDLLIATGR
                     HSIAASVLVKQLSGGRTKTVQLQNPVIDSNRFDLVVTPRHDRLEGRNVISTRGALHRL
                     TPELLREGAQRLAFQVMRLERPFIGVLIGGTNAAYRFGQSEATALARQLAACARAMNA
                     SILVTPSRRTDENCLRTLEIELLHVPHYIWDGQGDNPYFGILGLADFLVVTSDSVNMV
                     SEAANTGKPVYVVDLPGGSEKFSRFHNALREEGVTRPFAGTLSPYTYKPLDDVSMVVN
                     RVNLLLRE"
BASE COUNT         1035 a         1604 c         1619 g          886 t
ORIGIN      
        1 ctttcctggg cgagacgctt ttcctgcata tccagccagc tcgaataatt gccttcgtgg
       61 ggaatgccgc gtccgcggtc gagttccagg atccagcccg tcacatcgtc gaggaagtag
      121 cggtcatggg tgaccagcac gacggtgccg gcataatcgg acagatgccg ctccagccag
      181 gaaaccgatg ccgcgtcgag atggttggtg ggttcgtcca ggagcagcat gtcgggggct
      241 tcgagcagga gcttgcacag cgccacacgg cgcttctcgc cgccggagag attgttcaca
      301 tcggcctcgg ggtcgggaca gcgcagcgca tccatcgcct gctccacctg ggcgtcgagg
      361 tcccacagat tttgcgcgtc gatcttgtcc tgtagcgagg acatctcctc ggcggtctcg
      421 tccgtgtaat tggcggcgat ctcgttgtag cggtcgagca gagctttttt ctcggcgaca
      481 ccctccatca cattgccgag cacattgagc tgcggatcga gttcgggttc ctgcggcaga
      541 tagccgacgc gcacgccgtc ggcggcccac gcttcaccgt tgaactcctt gtcgacgccc
      601 gccatcatcc ggagcagcgt cgacttgccc gcgccgttca cgccgacaat gccgatcttc
      661 gcgccaggca ggaaggagag ccagatatcc ttgagaatct gacgcccgcc gggaaaggtc
      721 ttggaaagac ccttcatcac atagacatat tgcgggccag ccatgcggat actcctgaac
      781 gcgaaaggag cggggttgta gcgcaaaccg cgctacggca gcaatgcgat tggcaagcgc
      841 tatgggatca gaagttcagc tttgcccgcg caacgccggt cacggcgttc tggccgcgtg
      901 tgccagcggc attgagctga taggcggcgt tggcttgcag cgccagcgtg ccgtcgagga
      961 aggtggtcac atagccaagc tcgacatcgg attcgcgcgt ctgagtgttg gccagggcga
     1021 aattcgtatt cagcgcgccg aaccccacgc tgccggtata aatctgcagc gggcgcgaca
     1081 gcgcgatgcc gaatacgtca tcgccgaaca atcctttttt cgccagtccg atgccatagg
     1141 cttccgaatg caccggatcg cccccgccgc tggtcaccag cctgtcgcgg gaaagatcga
     1201 gctgcgtcac gccctcggca taggtcagcg tcgtgatcca gccttcgccg aatccaaccc
     1261 gcgccgaaat gcccaatgcc gagctgtcgg tcgagaccgc gcctctgagc gaggccggaa
     1321 tattgctgag cagcgaacca tcgccgttcg cgtgcgacgc cgtcaggccg aggccgcccc
     1381 aatcgctgaa attccaattg aggttcgcgg atgtggttcc gacactttgc agatccgagc
     1441 ccagcctctg ggcgaggtcg cgcgccaagc cgctcggctg cgcatcgctc agcgcgccaa
     1501 ggccgagcgc cacatgactg gcatccagag tgacaccctt gccgaggaca aagctcatac
     1561 ttgcgcgcgt attggtgcct gtcagatagg cggagctgag cgcggatgca gcggggaaga
     1621 ggtcggcata gggatcccaa ttgcggccac gcgacagttg aatctcaaca ccattggcaa
     1681 tgccaatcgc gttcggacgc agggcgggga catcctctgc caagcccgat gtcatcaggc
     1741 cgccgagatt gagattgacc gcatgagcgt tgcggtcagg cgaaacggag gacaacggcc
     1801 ccaaaatcgc ggatggcgca gccgcatagg ccagcggcac ccataaggcg cccgccagca
     1861 cggacaatgc tgctgtcgcg acccgcttgc gcataaaccc cgctactccc gccagcccat
     1921 actccggcgt cttccagtgt atcagctcaa ttccttatag gaaatgaacc ttgcgcgaca
     1981 aagctccctt tggtcgcagg gtggtgcttt tcggcactgc ggtaacaaaa ttgccaatat
     2041 catcgatagt tgaagcactt agttcagcca ttcgccgcca tgcggcacag aaaaaatcct
     2101 ctatggtttt gacgcaacac cggcactggg cttaacgata ttcctggcca cgagatcgcg
     2161 gattgtttcg ggtctgaagc ccgcctcctg caggatcgat tcactcccct ccccgagtct
     2221 gggcgcgggc cggtcaggct gccgcggcag gtccgcgacc cggaatggcg agcgaattcc
     2281 gagcatttgc ccctcggcgg ggtggacgaa atcttcgaac agcccggcgt ctttcagatg
     2341 gggctcctcg aacagcgctt cgaggctgtt gaccggaccg caagggatat cgcgttcgtt
     2401 cagaagcacc agccattcgg cggtgctatg cccgggcgcg gcatcggcga tcatttgata
     2461 gagcgcatcg acattcgcgc tgcgctgcac cgggtccttc acccagtccg cggccagaag
     2521 gtcgccacgg ccgacgcact cgaggaagcg cgcccattgc tgggtggtgt agggcatgat
     2581 ggcgatatag ccatcgcggg ttttgtaagg acgccggttc ttcgcgagca tccgttcata
     2641 gccgggcgga cccaaaggcg gcacgaagct gcgcccggcc agatgctcga ccagaagaaa
     2701 tgcgaccatg ctttcgaaca tcggggcttc gatggcgcaa ccctcgcccg tcttcagccg
     2761 gtggaccaat ccggtcgcga ccgcgaaggc aagatgcagc ccaaccacct tgtcggcgac
     2821 gatggagggg agaaagcgcg gcgccccagt ggaatcggcg ttgagttgag ccagacccga
     2881 catggcctgg atgatgtcgt cataggcggg ttccgcggca tagggtccgt tctgtccgaa
     2941 cccgggcgaa tagcaataga cgatgcgcgc attggcgcgc ttagcgtctt cgtaggcgag
     3001 accgagtttt gcggccgcgg cggaacgcat gttgtggacc agagcgtcgg caccgccaag
     3061 aagcgcaaga accacatcgc ggccttcggg ggctcgcaag tcgatggcga tggaacgctt
     3121 gttgcgattg gtattcagga agccggcccc catgccgtcg ctccggcttg gacgcggcga
     3181 gcggccgacg tcgccatcca gcgattcgac cttgatgaca tcggccccca tgtcgccgag
     3241 gaactgggtc gcgtaggggc cgagataggt cgtcgtcaga tcgatgacgc gcaccccttt
     3301 cagcagctca tacatcagcc ataggccttc acgaaataat agacggaaaa cagcgcgccc
     3361 gcggctgtga tgatgacacg caacgcttca ttcggaatgc gccgggccac gcgggcgcca
     3421 aaatagcccc cgccgatcgc gcctgccatg acgatcagcg cgacattcca ggccaccgag
     3481 ccggtgacta tgaaaatcag cgccgccacg ccattgatga aacagacaag aaaattgcgc
     3541 tgggcattgg cgctttgtat gtcgcgttcg cccgcgagcg ccatcgaggc cagcagcaga
     3601 atgccgatgg ccgcaccgaa atagccgcca tagatcgcgc tgacgaattc catgccgagg
     3661 agccgggtct cgttcttgcg gtgaggatcc ttggtcacgc gcctcacgat ccgccgcgaa
     3721 aaggtaaaca gggccgtggc ggcgcccagc aaccacggca ccagatagaa gaacaccgca
     3781 ttgccggaga cgagcagcag gacggccccc agcaagccgc cagccgtcgc ggccacaatg
     3841 cgctttggaa gatgctcgcg gaggcggccg agttcgggca ggtaagcata ggcggatgtc
     3901 aacgtgccgg gcacgagcgc aaccgtggtc gtggtgttcg ccaggaccgg cggcagcccc
     3961 gccgccaaca gcaccggaaa ggtgaagagc gtgccgccgc cgacgagcgc gttgcacgtc
     4021 ccggcgagaa aaccgaccgt tacgagaaga aggatttcgg cgggcgccac gggaaaccga
     4081 gagaagagac ttccgaacgc ttagtatgcg ggcgctattc ccgcaacagc aggttcacgc
     4141 gattgacgac catgctcaca tcgtccagcg gtttataggt gtagggggaa agcgttcccg
     4201 cgaagggcct ggtcacgccc tcctcgcgaa gcgcgttgtg gaagcgcgag aatttctccg
     4261 acccgcccgg caaatcgacg acataaaccg gctttccggt attggcggcc tccgaaacca
     4321 tattgaccga atcgctggtg acgacgagga aatcggcaag gccgagaatc ccgaaatagg
     4381 gattatcccc ttgcccgtcc cagatgtaat gcggcacgtg cagaagctcg atctccaagg
     4441 tgcgcaggca gttctcgtcg gtgcgccgtg aaggggtgac aagaatgctc gcgttcatgg
     4501 cgcgcgcgca tgccgcaagc tggcgcgcga gtgccgtggc ctcgctttgc ccgaagcggt
     4561 aggccgcgtt ggtgcccccg atcaggacgc cgatgaaggg ccgctccagc cgcatgacct
     4621 ggaacgcaag cctctgcgca ccctcgcgca gcaactcggg cgtcaggcgg tgaagcgcgc
     4681 cacgggttga gatgacgttc ctgccttcca gccggtcgtg gcggggcgtc accacgagat
     4741 cgaaacgatt ggagtcgatc acgggattct ggagctgcac cgtcttggtg cgcccgccgg
     4801 acaattgctt caccaggacg gacgccgcga tgctgtgacg ccctgtggcg atgagaaggt
     4861 ccggccaagg cggcgcgagg atgtcgctgc cgccagcgaa ttgcgcgcgt ccgccgacac
     4921 gcagataggg cgtcaattcc cgccatggcc agcgcagctt cacgcgtttg acgaccgggg
     4981 tgaggcccag cgcctcggcc aaaccgacgc attggctttc catgcccgct ttgccgtcgg
     5041 tcacgaccca acaggaaagc gggggagctt cagaacccaa acatgtgatc caggctgaaa
     5101 gcgcgcccgc cgatgaggcc gtgatagccg aacagccggc atgt
//
LOCUS       DASYEG010000241         3019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3132545, whole genome shotgun sequence.
ACCESSION   DASYEG010000241 DASYEG010000000
VERSION     DASYEG010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3132545"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VGM72_10415"
     CDS             complement(<1..272)
                     /locus_tag="VGM72_10415"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEY4265722.1"
                     /translation="MKNIGGTWDRAFFARDVLAVARDLIGVTLMVGNAGGLIVEVEAY
                     DRTDPASHCYGGRRTPRNESMFGPAGHAYVYRSYGVHWCLNFTCGA"
     gene            complement(269..1438)
                     /gene="solA"
                     /locus_tag="VGM72_10420"
     CDS             complement(269..1438)
                     /gene="solA"
                     /locus_tag="VGM72_10420"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HEY4265723.1"
                     /translation="MDRYDAAILGLGAMGSAAAYQLAKRGLRVLGLDRFAPPHPFGSS
                     HGDTRITRLAIGEGAHYTPLALRSHEIWREIEKETGTDLLTVTGGLVVSSAASKSRVH
                     VADFFANTVAAAQKHGIAHEILEGPELRKRFPQFNIGNGETGYYEPGAGFLRPENCVA
                     AQLGLARKHGATLHTDEKVLGFDATLDGVIIETDRGKYSAETLILAAGAWLPELLPER
                     YAANFRVRRQVLFWFDVSGPIAPFMPGRCPVFIWEVPGAPQGIYGFPAIDGPRGGVKI
                     ATEQYEMATTPQGVNRAVSPAETSVMYETYVAPYLPGVGAKCIKAVVCLYTVTPDAGF
                     VIDRHTESDRIIIASPCSGHGFKHSAAIGEVLADLAIDGRSRFDLSAFRLARFIS"
     gene            1498..2370
                     /locus_tag="VGM72_10425"
     CDS             1498..2370
                     /locus_tag="VGM72_10425"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY4265724.1"
                     /translation="MNIRDVLPIDVKPAGAALAAEIGGVDLRRLDDASFAAIHRAWAE
                     HLVLLFRGQQLSDDDLIAFSRRFGALDFAPVQETGRRFVEGHPEIYVVSNVIENGVPI
                     GSLGAGEAVWHTDMSYIADPPKASMLYALEVPSTGGDTGFVNMYAVYEALPLSLKRRL
                     DGLSLKHDGTYNSGGYVRQGIDAVDDPLTSPGAVHPIVCTHPETGRRVIYLGRRRNAY
                     IVGLPLAESETLLDELWTYAERAEFTWYNRWRVGDLVLWDNRCTMHRRDSFNGGARRV
                     MHRTQIKGETRPRA"
     gene            2367..>3019
                     /locus_tag="VGM72_10430"
     CDS             2367..>3019
                     /locus_tag="VGM72_10430"
                     /inference="COORDINATES: protein motif:HMM:NF013473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4265725.1"
                     /translation="MSRIRLRIRPPTIVLALLCAMYFLMFINRTNMAIAGPLMQADLN
                     LSNTDLGLAFSAFAYPYALFQLFGGMLGDRFGPRATLAISAFIVFLATAWIGAAGGLV
                     SLFAARILLGIGEGAAFPTATRAMSVWTPTGRWAFAQGITHTFSRVGNWATALIIAGL
                     IAILSWRASFYLLAPVNLIWMVVWFWYFRDSPSAHRAMRAEDIAALPVRARGDHKRK"
BASE COUNT          554 a          965 c          890 g          610 t
ORIGIN      
        1 tcggcaccgc aggtgaaatt caggcaccaa tgcacgccat aggagcgata gacatacgcg
       61 tgtccggcag ggccgaacat ggattcattg cgcggcgtgc ggcgtccgcc atagcagtgc
      121 gacgcggggt ccgtgcgatc ataggcttcc acctccacga tcaacccgcc tgcattgccg
      181 accatcaacg tgacgccgat aagatcgcgt gccaccgcga gcacatcgcg ggcaaagaac
      241 gcgcggtccc atgtgccgcc gatattcttc aagatatgaa cctggcgagc cgaaaagcgc
      301 ttaggtcaaa acggctcctg ccgtcgattg cgagatcggc aaggacctcg ccgatggcag
      361 ccgaatgttt gaacccgtga cccgagcagg gcgaagcgat gatgatgcgg tccgattccg
      421 tatgcctgtc gatcacgaaa cccgcatcgg gtgtgaccgt gtagaggcac accacggcct
      481 tgatgcattt ggcacccacc cccggcagat agggtgcgac ataggtctca tacatgacgg
      541 atgtttccgc gggcgatacc gcgcgattga cgccctgggg tgtggtcgcc atctcatatt
      601 gttcggtcgc gatcttcacg ccgccccgcg gaccatcgat cgctggaaaa ccatagatgc
      661 cttgcggcgc acccggcacc tcccagatga agacgggaca gcgccccggc ataaacggcg
      721 caatgggacc gctgacatcg aaccagaaca gcacctggcg ccgcactctg aaattcgccg
      781 cgtaacgctc aggcagcaat tccggcagcc aggcgcccgc agcgaggatc agcgtttcgg
      841 cggaatattt tccccgatcc gtctcgatga tcacaccatc gagagtggca tcgaaaccca
      901 acaccttttc atcggtgtgc agtgtcgcgc catgtttgcg ggccagcccc aattgcgcgg
      961 cgacacaatt ttccggacgc aggaaacccg cgccgggttc gtaatagccg gtctccccat
     1021 tgccgatgtt gaattgcgga aagcgcttcc ttagttcggg gccttccaga atctcatgcg
     1081 cgattccatg tttttgcgca gccgccaccg tattggcgaa gaaatccgca acatgcaccc
     1141 tggatttgct cgcagcactg gagacgacca gcccgccggt caccgtcaac aaatcggtgc
     1201 cggtttcttt ctcgatctcc cgccagattt cgtgcgagcg cagcgccagg ggcgtgtaat
     1261 gcgcgccttc cccgatggcg agccgggtga tgcgcgtatc gccatgggag gacccgaatg
     1321 ggtgcggcgg cgcaaaccga tccaggccaa gcacacgcag cccccgtttg gccaattgat
     1381 aggccgccgc acttcccatc gcacccagcc ccaaaatcgc ggcatcgtat ctgtccatgc
     1441 ccgattgtaa cctgtttcct ctcaaggcaa gctggcagaa agggagcatc cgaaacgatg
     1501 aacattcgtg acgtcctgcc tatcgacgtg aaacctgcgg gagcggcatt ggccgccgaa
     1561 atcggcggcg ttgacctgcg tcggctggac gacgcgtcct tcgccgccat ccatcgcgcg
     1621 tgggcagagc atctggtcct gctctttcgc ggacagcagc tttccgacga cgatcttatc
     1681 gcgttcagcc ggcgcttcgg cgcgctcgat tttgcaccgg ttcaggagac cggccggcgt
     1741 ttcgtcgaag gccatccgga aatctatgtc gtctccaacg tcatcgagaa cggcgtcccc
     1801 atcggcagcc tcggcgcggg cgaagcggtc tggcacacgg atatgtccta tatcgccgat
     1861 ccccccaagg cgagcatgct ctatgcgctg gaggtgccgt ccaccggcgg cgataccggc
     1921 ttcgtcaata tgtatgccgt ttatgaggcg ttgccgcttt cgctgaaacg gcgcctcgac
     1981 ggtctttccc tgaagcatga cggcacctac aacagcggtg gttatgtccg tcaggggatc
     2041 gatgcggtgg acgatcccct cacctcgccc ggggcggttc atccgattgt ctgtacccat
     2101 cccgaaaccg ggcggcgcgt gatttatctc gggcggcggc gcaacgctta tatcgtgggc
     2161 ctgccgctgg ccgaatccga aacgctgctc gatgaattgt ggacctatgc cgagcgcgcg
     2221 gaattcacct ggtacaatcg ctggcgcgtg ggcgatctgg tgctgtggga caatcgctgc
     2281 accatgcatc ggcgcgattc cttcaatggg ggcgcgcggc gcgtcatgca tcgcacacag
     2341 atcaaaggcg aaacgcggcc tcgcgcatga gcaggatccg cttgcgcatt cgccccccaa
     2401 ccatcgtgct cgcactgctg tgcgcgatgt atttcctgat gttcatcaat cgcaccaata
     2461 tggcgattgc cggtcccctc atgcaggccg acctcaatct gagcaacacc gatctcggtc
     2521 tggcgttctc ggccttcgcc tatccttatg cactgttcca attgtttggc ggcatgcttg
     2581 gcgatcgctt cggcccgcgc gccactctgg cgatttcggc cttcatcgta tttcttgcca
     2641 cggcgtggat cggggcggcc ggcggcctcg tctcgctgtt cgccgcgcgc attcttttgg
     2701 gcatcggcga aggtgctgcc ttccccaccg ccacgcgggc catgtcagtg tggacgccta
     2761 cggggcgctg ggccttcgcg caaggcatca cgcacacatt ttcgcgcgtc ggcaattggg
     2821 ccacagcgct gatcatcgcg ggattgatcg cgattctctc ttggcgtgcg tcgttctatc
     2881 tgctggcgcc cgtcaatctg atctggatgg tggtgtggtt ctggtatttt cgcgattcac
     2941 ccagcgcgca tcgcgcaatg cgcgctgagg atattgcggc gcttccggtg cgcgctcgcg
     3001 gcgaccacaa gcgcaagat
//
LOCUS       DASYEG010000242         9235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3277623, whole genome shotgun sequence.
ACCESSION   DASYEG010000242 DASYEG010000000
VERSION     DASYEG010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 9235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9235
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3277623"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VGM72_10435"
     CDS             complement(<1..288)
                     /locus_tag="VGM72_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265726.1"
                     /translation="MVEYRAWSLPGLAGALLLLAAASDDARPDSPGLRVTAEALEGPV
                     SAVVQRVVDGDTIEVRAAIWLGQSLTVRVRIDGVDAPELKARCPEERQLAIA"
     gene            complement(359..577)
                     /locus_tag="VGM72_10440"
     CDS             complement(359..577)
                     /locus_tag="VGM72_10440"
                     /inference="COORDINATES: protein motif:HMM:NF019511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1674 domain-containing protein"
                     /protein_id="HEY4265727.1"
                     /translation="MAKDEKADDGPSPRRELPPAAVRALAEAAERRKLAEQAEKAGGP
                     KPKELNGPKGPEPTRYGDWERKGIASDF"
     gene            668..1540
                     /gene="htpX"
                     /locus_tag="VGM72_10445"
     CDS             668..1540
                     /gene="htpX"
                     /locus_tag="VGM72_10445"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF002363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEY4265728.1"
                     /translation="MNLVRTGVLMAGLTGLFVAIGYVIGGQTGMVMAFLFAVGTNLFA
                     YWNSDKAVLSMYGAREVDESSAPDLVHLVRTLSQKAGLPMPKVFIAENPQPNAFATGR
                     SPEHAAVCVTTSILQRLNEEELAGVLAHELSHVKNRDTLTMTITATIAGAISMLANFA
                     FFFGGDRRNNQLGAVGTILIMILAPLAAMLVQMAISRTREFEADRSGAMLSGRPLWLA
                     SALRQIASDATHIENVEAETHPATAHMFIINPLHGGNFGNLFASHPSTEERIARLKSI
                     AAQLGQAPQPGPWS"
     gene            1531..2868
                     /locus_tag="VGM72_10450"
     CDS             1531..2868
                     /locus_tag="VGM72_10450"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEY4265729.1"
                     /translation="MELSGETQAFPPGLAARKAALTILSDVLRNKRNLDAAAADAQAR
                     AKLEARDAGFARAIATASLRYFGQLEALIRSFVPKAPPPHKAGPTLEILIAGACELLI
                     LNIPAHAAVDAANRLAQGDAKAVHFKALINAVLRRVAREGAAIVAGQDAAALNTPDWL
                     WPRWVSHYGEQTARAIAQAHRGIPPLDLTLKSPDDPYTDITGIAALARGRLRLTHAGR
                     IEDVSGFHDGRWWVQDFAASLPVRLLGDVAGKAVLDLCAAPGGKTAQLAALGARVTAV
                     DAASDRLTRLRENLGRVNLTAELAAADVRDWHPASPAPFVLLDAPCTATGTIRRHPDL
                     PWAKGAADLMMCETLQSELLDAAAGMTALDGTLIYAVCSLEPEEGEEQIGAFLRRRTD
                     FQRVPVTAEEVFDAAFVSSDGDLRTLPCHWADRGGMDGFYAARLRRYSSRPSA"
     gene            complement(2844..4850)
                     /locus_tag="VGM72_10455"
     CDS             complement(2844..4850)
                     /locus_tag="VGM72_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265730.1"
                     /translation="MGAFWAGTATAYLWGYLASQAASSIDPQVLAFAAMAILLPPLLF
                     IAAAFALARALTLSDTARQLASVSSRLTEADEAAVQNAQRLGRAVRRELDTLSTGLDG
                     AFGRFRALETALEERIAQLEDAGARAGVRAETIAQRLQSEREGIEELAGRLDEAAART
                     AEILAGRNAQLKTIIEDAGGELKAAGQTLDAQVAQFRDAAESAARAPLTAALELDRQA
                     KEIENAGEAAAARAEFVLARQERQRLAMSELLVRLREEAENFNGVMQTQRNNVEMAAN
                     VLNGETQRLDDVATAGMHRLDAAIVHAGATFTDQTQRLEAITESAIRRLDETMVQTAT
                     RSAQLAASFGSDANRVREVSDGAASGIARLVESLREAATSAHALFADSMEAAKRRSID
                     FVGDAMAQCDALLRAAAAVTQETEKARAALAHAAEDAERHIVALPGIAQQEAQRVRET
                     VRSETELMLDISARAITTLQTRSGRRQIPEQPHAQQPEAEAAPVSEGVGEGLRGLARR
                     ITAPKRRAEDRSSEASKGRYDLSAVLAAADAGTKPGLRPGAAGALAALQAALADLAGD
                     LEELAGETADPVLWRRYVDGDRAVFARRLAASIGPESVNRITELYRENPRFHDSAETY
                     MAEFEVMLGRAREGDRDGFLASTLLSADTGKIYLAIAYALGRLE"
     gene            5226..5555
                     /locus_tag="VGM72_10460"
     CDS             5226..5555
                     /locus_tag="VGM72_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265731.1"
                     /translation="MGEAGDGPRSESEPFAGGSDAEVANGRPNPAQRRYLARGLTQAG
                     GKLPLFDEYGQHIHPALVRACIAHGWAEPWFGNPLKADWLVCRLTQKGRDILATKEGH
                     ATKEGGH"
     gene            5609..5971
                     /locus_tag="VGM72_10465"
     CDS             5609..5971
                     /locus_tag="VGM72_10465"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY4265732.1"
                     /translation="MARTDKPRTDQPVDLDHLDRYTGGDRAINQEILRLFDDQCRESL
                     AMLEYLAQGSGDSQSWREIAHRLKGAARGIGAFALGDAAAVAEKTAPEEALAVLERLR
                     DDAAAVHNLIAEFLKASA"
     gene            6098..7147
                     /locus_tag="VGM72_10470"
     CDS             6098..7147
                     /locus_tag="VGM72_10470"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY4265733.1"
                     /translation="MSTIPDAETGSLATDVIIIGAGPIGLFAVFELGLLDLKCHLVDI
                     LDRPGGQCTELYPEKPIYDIPGIPIVTGQELTDRLLEQIDPFHANFHFGEMASALARL
                     PDGRWQLTTDAGTVLTAPVIVIAAGGGSFVPKRPPVPGIEAYEGKSVFYAVRKMEHFR
                     DKDVLIAGGGDSALDWTLNLAPIAKSLTLVHHRDGFRAAQHSVNGMRALVDAGKIAFH
                     VASVKALHGAHGQLEAVTLAGADKKEWRQEANALLPFFGLTIKLGPIAEFGLNLHENL
                     IPVDTAKFESGTPGIFAIGDINTYPGKLKLILSGFHEAALMAQAAFHICRPNEKLRFQ
                     YTTSSSSLQKKLGVA"
     gene            7157..7465
                     /locus_tag="VGM72_10475"
     CDS             7157..7465
                     /locus_tag="VGM72_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020043927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY4265734.1"
                     /translation="MKIIARDRKGVEHIVEGNDGWTVMEALRDAGLPITAECGGACAC
                     ATCHVYVEDGWYEKLKAPSPEETDMLDMALAVEPNSRLSCQITVNAATDGIKVTLAPE
                     "
     gene            complement(7446..8327)
                     /locus_tag="VGM72_10480"
     CDS             complement(7446..8327)
                     /locus_tag="VGM72_10480"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEY4265735.1"
                     /translation="MRWDQGNRYGGIEERRGMGGVGLAGGGLGAAVLGLVGYFFFGIN
                     PLVIMDAVNQVAPQTQQEGRTGTPQDRLGQFVDTIESSTTDVWRVEFQKMGRTYQPPN
                     PLVLYDQATGTQCGTGESAMGPFYCPRDFRVYLDLQFFGELSTRFGAPGDFAQAYVIA
                     HEIGHHVQNLLGVSDKVEQAESRAPNRSVANRYSIALELQADCYAGVWAAHASEVSGG
                     RVALEPGDLQEGLRAAAAVGDDTLQKQTQGRVVPDSFTHGTSAQRQHWLEQGYTTGDP
                     KACDTFAATRGIIPGRG"
     gene            8436..8663
                     /locus_tag="VGM72_10485"
     CDS             8436..8663
                     /locus_tag="VGM72_10485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265736.1"
                     /translation="MNISLKVTQIGNSLGLILPKEMLTTLKVGKGDTLICSETSNGFQ
                     ITPYDPEVARQLEAGREFAKEYRDTLRALAK"
     gene            8660..9052
                     /locus_tag="VGM72_10490"
     CDS             8660..9052
                     /locus_tag="VGM72_10490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEY4265737.1"
                     /translation="MSEPAWISRRALELLHQESLAEHGGADGLRDSGLLDSALALARA
                     QNIHSYENVGDPFRLAAAYAFGVAKNHPFVDGNKRAAFIAAGLFLRINGVRLNADKVQ
                     AVLVMLDLAAGKMTEAEFAEWLRKNARA"
BASE COUNT         1643 a         2963 c         2904 g         1725 t
ORIGIN      
        1 cgcaatggca agctgccgtt cttcggggca gcgcgccttc aactccgggg cgtcgacgcc
       61 atcgatgcgg acacggacag tcaggctctg tccgagccag atggcggcgc gcacttcgat
      121 ggtgtcgcca tcgacgacac gctgcaccac cgccgagacc ggcccctcca gggcctcggc
      181 tgtcacacgc aagcctggag aatccgggcg ggcatcatcc gacgccgccg ccaaaagcag
      241 cagggcgccg gccagccccg gcaaactcca agcacgatat tcgaccattg acgggaatcg
      301 gcatagcgtt acaatttatg attgtagtac aaatcacgaa caaatatcca tagggcgatt
      361 aaaaatccga ggcaatgcct tttcgctccc aatcgccata gcgcgtgggc tccggtccct
      421 tggggccgtt cagctctttg ggcttgggtc cgcccgcctt ttcggcttgc tccgccagct
      481 tgcggcgttc ggccgcctcc gccagtgccc ggacggcggc gggcggcaat tcgcgcctgg
      541 gtgatggtcc atcatcggcc ttctcgtcct tcgccatgca agaccctttg ccaaaacttc
      601 gcagctccct acataaacag cctcgggtgc cgcaaacagg ggcagccaaa cgggttgagg
      661 cgttttcatg aatctcgttc ggacgggagt tctgatggcc ggtttgaccg gcctctttgt
      721 tgcgatcggt tatgtcatcg gcggccagac cggcatggtc atggcgttcc tgttcgccgt
      781 gggcaccaat cttttcgcct attggaattc ggacaaggcg gttctttcca tgtatggcgc
      841 acgggaagtc gacgaatcgt ccgcgcccga tctggtgcat ctggtccgta cgctgtcgca
      901 gaaagccgga ctgccgatgc ccaaggtctt catcgccgag aatccgcagc ccaacgcctt
      961 cgcgaccggc cgcagcccgg agcatgccgc tgtgtgcgtg acgaccagca tccttcagcg
     1021 gctgaacgag gaagaactgg cgggcgtgtt ggcccatgaa ctttcccatg tgaaaaatcg
     1081 cgacacgctc accatgacga tcaccgcgac catcgcgggc gcgatttcca tgctcgccaa
     1141 tttcgcgttc ttcttcgggg gcgaccggcg caacaatcaa ttgggcgcgg tgggcacgat
     1201 cctcatcatg atcctggcgc cgcttgcggc catgctggta cagatggcca tcagccgcac
     1261 gcgcgaattc gaggccgacc gcagcggcgc gatgctctcc gggcggccgc tgtggctggc
     1321 ctccgcgctg cgtcaaatcg cgtcggacgc cacccatatc gagaatgtgg aggccgagac
     1381 gcaccccgcg accgcgcata tgttcatcat caatccgctc cacggcggaa atttcggcaa
     1441 tctcttcgcc tcccatcctt cgaccgagga gcgcattgcg cggctcaaat ccatcgccgc
     1501 ccaactcggc caggcgccgc aacccggccc gtggagttga gcggggagac gcaggcgttc
     1561 ccaccgggct tggcggcgcg caaggcggcg ctcacgatcc tgtccgatgt gctgcgcaac
     1621 aaacgcaatc tggatgccgc ggccgccgat gcgcaggcgc gtgcgaagct tgaggcccgc
     1681 gatgccggat tcgcgcgcgc catcgcgacc gcttcgctgc ggtatttcgg tcaactcgaa
     1741 gcgctgatcc gcagcttcgt gcccaaggcg ccgccgccgc acaaggccgg cccgacgctg
     1801 gaaatcctga ttgcgggggc ctgcgagctt ctgatcctca atatcccggc ccatgcggcc
     1861 gtggatgccg ccaaccgcct ggcgcagggc gatgcaaagg ccgtgcactt caaagcgctg
     1921 atcaatgcgg tgctgcggcg cgtcgcgcgc gaaggggcgg ccattgtcgc aggtcaggat
     1981 gcggcggcgc tgaatacgcc cgattggctg tggccgcgct gggtgtcgca ttatggcgaa
     2041 cagacggcgc gcgccatcgc gcaggcccat cgcggaattc cgccgctcga cctgacgctg
     2101 aaatctccag acgatccgta tacggatata acgggaatcg ccgcgcttgc gcgtggccgc
     2161 ctgcggctga cccatgccgg ccgcatcgaa gatgtgtcag gttttcatga tggccgctgg
     2221 tgggtgcagg atttcgcggc gtccctgccg gttcggcttc tgggcgatgt ggcggggaag
     2281 gccgtcctgg atctctgcgc cgcacccggt ggcaagaccg cgcaactcgc agcattgggc
     2341 gcgcgcgtga ccgccgtgga tgccgcgtcc gaccgtttga cgcggttgcg tgaaaatctc
     2401 ggccgcgtca acctgacggc ggagcttgcc gccgccgatg tccgcgattg gcatcccgcc
     2461 agcccggcac ctttcgtgct gctcgatgcg ccctgcaccg ccaccggcac gatccgccgc
     2521 catcccgatc ttccatgggc gaagggtgcc gccgatctca tgatgtgcga aactctgcaa
     2581 agcgagctgc tggacgccgc ggccggcatg acggcactgg atggaacgct catctacgcg
     2641 gtctgttcgc tcgaacccga agagggcgag gagcagatcg gcgcattcct ccgcagacgg
     2701 acggattttc agcgcgtccc cgttacggcc gaagaggttt tcgatgccgc cttcgtctcg
     2761 tccgatggcg atctgaggac gctgccgtgc cattgggcgg acagaggcgg catggacgga
     2821 ttttacgccg cgcgcttgcg acgctattcc agccggccga gcgcgtaggc gatggcgagg
     2881 tagatttttc cggtatcggc gctcaggagc gtcgaggcga ggaacccgtc gcggtcgcct
     2941 tcgcgcgcgc ggcccagcat gacttcgaat tcggccatat aggtttcagc cgaatcgtga
     3001 aagcgcggat tctcacgata gagttcggtg atgcgattga ccgattccgg tccgattgag
     3061 gccgcgagcc ggcgcgcgaa gacggcgcgg tcgccatcga catagcgccg ccacaaaacg
     3121 gggtccgcgg tttccccggc gagttcttcc agatcgccgg ccaggtcggc gagggcggcc
     3181 tgaagcgccg ccagcgcacc ggcggcaccg ggtcgcaatc cgggcttggt gcccgcatcg
     3241 gccgcggcca acacggcgga aagatcatat cgcccctttg aagcctcgga gctgcgatcc
     3301 tcggcgcgcc gcttgggcgc ggtgatgcgg cgcgcaagac ctctcagtcc ctcgccgaca
     3361 ccctcggaga cgggcgcggc ttcggcttcc ggctgctgcg cgtgcggctg ttccggtatc
     3421 tgccggcgcc cgctgcgcgt ctgcagcgtg gtgatggcgc gcgctgagat atccagcatc
     3481 aattcggttt cgctgcggac ggtttctcgc acgcgctggg cttcttgctg ggcgattccg
     3541 ggaagcgcca cgatatggcg ctcggcgtcc tcggccgcat gcgccagagc cgcccgcgcc
     3601 ttttcggtct cctgggtcac cgccgccgcc gcgcgcagca gcgcatcgca ttgggccatc
     3661 gcgtcgccga cgaaatcgat ggaccggcgt ttggcggcct ccatgctgtc cgcgaaaagc
     3721 gcgtgcgcgc tggtcgccgc ctcgcgcagc gattccacca gacgcgcaat gcccgaagcc
     3781 gcgccgtccg aaacctcccg gacgcgattc gcatcgctgc cgaagcttgc cgcgagctgc
     3841 gccgaacgtg tcgcggtctg gaccatggtt tcgtccagcc ggcgtattgc cgattcggta
     3901 atcgcttcga ggcgctgggt ctggtccgtg aacgtcgcgc ccgcgtggac tatcgcggcg
     3961 tccaggcgat gcattcccgc tgtggcgaca tcgtccaggc gttgggtttc gccgttcaac
     4021 acattggcgg ccatctccac attgttgcgc tgcgtctgca tcacgccatt gaaattttca
     4081 gcctcttccc tcagccgcac gagcagttcg ctcatcgcga ggcgctggcg ctcctgacgt
     4141 gcgagcacga attcggcgcg cgcggcggcg gcttctccgg cgttctcgat ttccttggcc
     4201 tggcgatcca gttcgagggc ggcggtcagc ggcgcgcgcg cggcgctttc cgccgcgtcg
     4261 cgaaattgtg cgacctgcgc atccagagtt tgaccggccg ctttcagttc gccgcctgca
     4321 tcttcgatga tggtcttcag ttgcgcgttg cgtccggcca ggatttccgc ggtgcgcgcg
     4381 gcggcctcgt cgagccggcc cgcgagttcc tcgatgcctt cgcgttcgga ttgaagccgc
     4441 tgcgcgatgg tctcggcgcg cacccctgcg cgcgccccgg catcctccaa ttgtgcgatg
     4501 cgttcttcga gcgcggtctc aagtgcgcga aaccgtccga acgctccgtc cagtccggtg
     4561 gacagcgtat cgagttcacg gcgcacggcg cggcccagac gttgcgcgtt ctgtaccgcg
     4621 gcttcgtcgg cttcggtcaa acggctggac accgaagcca gttgacgcgc ggtgtcggag
     4681 agtgtgagcg cgcgggcaag tgcgaaggcc gccgcgatga acaggagcgg aggaagcagg
     4741 atcgccatcg cagcgaaagc cagcacctgc ggatcgatgc ttgacgcggc ttgcgacgcc
     4801 aaatagcccc acagataggc agtggcggtg ccggcccaga atgcgcccat gacacatcca
     4861 aaggcgagga tggcccggcc ggttcgtggc gtttcggaga attccgggac accgcgggca
     4921 cgcgtttcct cgatgcgatg gacgccactt tgcggggttg cgtctttttg ggttgcggaa
     4981 tccttttccg cctcaggttc cggccctgtg atttgggtcc caatgacctg gggtcccgtg
     5041 acctgggatc ttaaactgac catctctgac cctattgcca ggcgcaggct cggcgcaccg
     5101 agccggaaat tccaagggat cggtgccgtt cggcaatccc tgggcgcaac tgcgattcac
     5161 atcttccgct cgacggtatt gaacaggagt tctatgtctt taggcaaccg caaaaggcga
     5221 acggcatggg cgaagccggg gacgggccgc gaagcgagag tgagcctttc gcgggcggca
     5281 gcgatgccga agtcgcaaat ggccgtccga accccgcaca gcgccgctat cttgcgcgcg
     5341 gtctgacgca ggcgggcggc aagcttccgc tgttcgacga atatggccag cacatccatc
     5401 ctgctcttgt gcgcgcctgc atcgcccatg gctgggccga gccctggttc ggcaatccgc
     5461 tgaaggccga ttggctggtg tgccgcctga cgcaaaaagg ccgcgacatc cttgccacaa
     5521 aagagggcca tgccaccaag gagggcgggc attaacgccg tcttgaagga cccgcgccat
     5581 gatcggggga agacgaggat tcttccccat ggccagaacc gataagccca gaacagatca
     5641 gccagtcgat ctggatcatc tcgaccgcta tacgggggga gatcgcgcca tcaatcagga
     5701 aatcctgcgc ctgttcgacg atcagtgccg cgaaagcctc gcgatgctcg aatacctcgc
     5761 gcagggatct ggcgattcgc aatcctggcg cgaaatcgcc cacaggctga agggcgcggc
     5821 acggggcatt ggggcgttcg cgcttggcga cgccgctgct gtggcggaaa agaccgcgcc
     5881 cgaagaggct ttggctgtgc tcgaacggtt gagagacgat gccgcggcgg tgcataatct
     5941 catcgccgaa ttcctgaaag cctcggcctg acggctgatg gcctgacggc gtgacgcagg
     6001 caagacatcc acaatcggat tgcagcccgg agtattttta catataaatt gcgtcacttt
     6061 ataaattcac acactaaagt tatgtaaaat gaacgatatg agcacaatcc cggacgcgga
     6121 aaccgggtcc ctcgcaaccg atgtcatcat catcggcgcc ggccccattg ggctgttcgc
     6181 cgtgttcgaa ctcggccttc tggatttgaa atgccacctg gtcgatatcc tcgaccgccc
     6241 cggaggccag tgcaccgagc tttatccgga aaagcctatc tatgacattc cgggcattcc
     6301 gatcgtcacc ggccaggagc tgacggaccg tctccttgag cagatcgatc cgtttcacgc
     6361 gaatttccat tttggcgaaa tggcgagtgc cctcgccagg cttcccgatg ggcgctggca
     6421 gctcaccacc gatgccggca ccgtcctgac cgcgcccgtg atcgtgatcg cagccggcgg
     6481 cggcagcttc gtgcccaagc gtccgccagt tccggggatc gaggcctatg aaggcaaatc
     6541 ggtcttctat gccgtgcgca agatggagca tttccgggac aaggatgtcc tgatcgcggg
     6601 cggcggcgat tccgcgctgg attggacgct caatctcgcg cccatcgcga aaagcctgac
     6661 gctcgtgcac catcgtgacg gattccgcgc ggcacaacac agcgtcaacg gcatgcgtgc
     6721 gctggtggat gcggggaaga tcgcctttca tgtcgccagc gtgaaagccc tgcacggcgc
     6781 gcatggtcag ctcgaagcgg tgacgctcgc gggcgcggat aagaaggagt ggcgccagga
     6841 agccaatgcg ctgttgccgt ttttcgggct caccatcaag cttgggccga tcgccgaatt
     6901 cgggctgaac ctgcacgaga acctgattcc ggtggacacg gcgaaattcg aaagcggcac
     6961 gcccggcatc ttcgccatcg gcgacatcaa cacctatccc ggcaaactga aactgatcct
     7021 atcgggcttt cacgaggcgg cgctgatggc gcaggccgcc ttccatatct gccgcccgaa
     7081 cgagaagctg cgcttccaat acacgacctc gtcctcgtcc ttgcaaaaga aactcggcgt
     7141 ggcgtaagcg ttccccatga agatcatcgc gcgcgaccgc aaaggcgtcg aacatatcgt
     7201 cgaaggcaat gatggctgga cggtgatgga ggctctgcgc gatgcgggcc tgccgatcac
     7261 ggcggagtgt ggcggcgcgt gtgcttgcgc cacctgccac gtctatgtcg aggacggctg
     7321 gtacgaaaaa ctgaaagccc cctcgccgga ggaaaccgac atgctggata tggcgctggc
     7381 ggtcgagccc aattcacggc tgtcctgcca gatcacggtc aacgcggcga ccgatgggat
     7441 caaggtcacc ctcgccccgg aatgattccg cgtgttgcag caaatgtatc gcaagccttc
     7501 ggatcgccgg tcgtataacc ctgttccagc caatgctgac gctgggccga ggtgccgtgc
     7561 gtaaaactgt cgggtaccac gcgcccctgt gtctgtttct gcagcgtgtc gtcaccgacc
     7621 gcggcggccg cccgcaagcc ttcctgtaga tcgcccggtt cgagcgcgac cctgccccct
     7681 gaaacttcgg acgcgtgcgc ggcccataca ccggcatagc aatcggcttg cagttcgagc
     7741 gcgatggaat agcgattggc gacgctgcgg ttcggcgcgc gtgattcggc ttgctcgacc
     7801 ttgtcactca cgccgagaag attctgcacg tgatggccga tctcatgggc gatcacatag
     7861 gcctgcgcga aatcgcccgg cgcaccgaag cgcgtggaca gctcgccgaa gaactgcaag
     7921 tccagataga cgcggaaatc ccgcggacaa tagaacggcc ccatggcgga ctcgcccgtc
     7981 ccgcattgcg tgccggtggc ctggtcatag agcaccagcg gattgggcgg ctgataggtg
     8041 cggcccatct tctggaattc gacgcgccac acatcggtgg tggaggattc gattgtgtcc
     8101 acaaactgcc cgagccggtc ctgtggcgtg ccggtgcgcc cttcctgctg ggtctggggc
     8161 gcgacctgat tgaccgcatc catgatgacg agcgggttta tgccgaagaa gaaatagccg
     8221 acgaggccga ggaccgccgc gcccaatccg ccacccgcaa gccccacgcc gcccatgccg
     8281 cgccgctcct cgatgccgcc gtaacgattg ccctggtccc agcgcatgga attctccgat
     8341 tcgtctcgat gctaacgcgt cagccggtga caggctgcct tgaacttgac gcctgtcaag
     8401 gagtgttata atgttcgtta taacaggaac gcgccatgaa catctctttg aaagtcaccc
     8461 aaatcggcaa ttccctgggg ctcatcctgc ccaaggagat gttgacgact ttgaaggttg
     8521 gcaagggcga cacacttatc tgttcggaaa cctcgaacgg attccagatc acgccctacg
     8581 atccggaagt ggcccggcaa ctggaggcgg gccgcgaatt cgccaaggaa tatcgtgata
     8641 cactgcgtgc gctcgcgaaa tgagcgaacc ggcgtggatt tcccgtcggg ctctcgaatt
     8701 gctgcatcag gagagcctcg cggaacatgg tggcgccgat ggattgcgcg attcgggctt
     8761 gctcgattcg gcactggcac tggcgcgggc acaaaacatc cactcctatg aaaacgtcgg
     8821 cgatccattc cggctcgcgg cggcttacgc ctttggcgtc gcgaagaacc atccgttcgt
     8881 ggatggcaat aagcgggcgg cgttcatcgc cgccggtctt ttcctgcgca tcaacggcgt
     8941 gcgcttaaac gccgataagg tgcaagcggt acttgtgatg ctggatcttg cagccggcaa
     9001 aatgacggaa gccgaatttg ccgagtggtt gcggaaaaac gcgcgagcct aatccacccc
     9061 gtcatccccc cgtcatcgcg aatcgcttat gcttcgctaa gggttggcgc aacgccgatg
     9121 ttccggctac agtcccgccc gcttttggtt cgcctcgcgc ctcaagggag agtgacgcct
     9181 tcagtacgga taggcaaacg tgctagccgc cgagcgtgtc cccgacaatt gcccg
//
LOCUS       DASYEG010000243         3164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_812282, whole genome shotgun sequence.
ACCESSION   DASYEG010000243 DASYEG010000000
VERSION     DASYEG010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812282"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..883
                     /locus_tag="VGM72_10495"
     CDS             74..883
                     /locus_tag="VGM72_10495"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4265738.1"
                     /translation="MFARLNHVAIVSENYAQLSKFYEAMFGMTTSSKTRPTRAVTVRD
                     GYVGLNINPRRAGRSAGFDHFGIQVEDSETAFDRLRKYYPSVKWQQRPANRPFAGITT
                     HDPDGNVFDISQKDMTNRTDVYVENDGRANPRHIDHFAMRTMHPAEMAEFYSRVFEFA
                     PVNRKEGDRNYCVTDGHVTLVIMPWDITDYAGTGIITCGMDHIGFKVESVDACKADIA
                     RIAADNPRLAPEPVGTGAEGGVLEQLFARSCPFGEHRLADSDGILLDIHAN"
     gene            896..1945
                     /locus_tag="VGM72_10500"
     CDS             896..1945
                     /locus_tag="VGM72_10500"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY4265739.1"
                     /translation="MRHWMRKLVTGAGLAWLALSPVAARADSVSDFYHGRQMIMILSA
                     DAGGGYGSYANAFAPYLAQHIPGKPRIVVQYMPGAGGLRAMNYLYSAAPKDGSTIALV
                     HSSVPFAPLYGLAAAKFDPRKINWIGSVNATSGICVAWATSNIKSWNDLFTKQFVVGS
                     SGAGSQMETIPAMLNKLFGTKIKVISGYRGGNEVYLAMERGEVQGRCGGLISSINSTR
                     PDWFPQKKVAVPIQIALERDPQFPDSPALIEFAKDARTKQILQLVLAPMAMDRPILTP
                     PGVPADRVAALKAAFHAAVNDPAFLADAKKQRLEVKEVSGEKVAGLLATAFAMPPDVV
                     KAANDAMGGAAGRGE"
     gene            1972..3027
                     /locus_tag="VGM72_10505"
     CDS             1972..3027
                     /locus_tag="VGM72_10505"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4265740.1"
                     /translation="MKRFIFALLAGLALGASSGALAQPSSVKPMTFPVVQGKTYKFEK
                     IADGVYYATGGFGSNNVVIVNDNDVMLVDTGTTPGNARRFVADVKMLTDKPIRYVVNT
                     HWHFDHTDGNQIFGPDVQIIGQEYLAKAITPDIVNREPFRTSTGPVIQAQMDMLKKQI
                     ADEKDTAKKAALNKQLADDQLLQSELKELKPTPPNKTYKDKLVIQSGAREIDLLFLGR
                     GHTEGDTVVYLPKEKIVATGDLMESRIAYMGDAVFDEWIDTLDKLKKLDFNVDLPGHG
                     IAFSNKGLITAFQDYIQDLIVKGNELKRAGMSADDMAKTIDLTSHAKDFPQITGPGMD
                     LRGARRLYAWLTERGGG"
BASE COUNT          654 a          960 c          977 g          573 t
ORIGIN      
        1 aattttcgct ctctggcggg tggctgcggt ttcgggtagc tttgggccca aaataacgag
       61 gcaggaggtg cgtttgttcg ccaggcttaa ccacgtcgcc atcgtgagtg aaaattacgc
      121 gcagctcagc aaattctacg aggcgatgtt cgggatgacg acctcgtcca agacacgccc
      181 gacgcgcgcg gtgacggtgc gcgatggtta tgtcggcctg aacatcaatc cgcgccgcgc
      241 cgggcgctcg gccggattcg atcatttcgg catccaggtg gaagattccg aaacggcttt
      301 cgaccgcctg cgaaaatatt atccgagcgt caaatggcag caacggcccg ccaatcgccc
      361 ctttgccggc atcaccaccc atgaccccga cgggaacgtg ttcgacatct cgcagaagga
      421 catgaccaat cgcaccgatg tctatgtcga gaacgacggt cgcgccaacc cccgccatat
      481 cgatcatttc gcgatgcgca cgatgcaccc ggccgaaatg gcggagttct actcccgcgt
      541 tttcgagttc gcgccggtga accgcaagga aggcgaccgg aactattgtg tcaccgatgg
      601 ccatgtgacg ctggtcatca tgccgtggga catcaccgat tacgccggaa cggggatcat
      661 cacctgcggg atggaccata tcgggttcaa ggtcgaaagc gtggatgcct gcaaggccga
      721 tatcgcccgc atcgccgcag acaatccgcg gctggcgccc gagccggtcg gaacgggcgc
      781 ggaaggtggc gtgctcgaac aattgttcgc gcgctcctgt cccttcggcg agcatcgcct
      841 tgccgacagc gacggtattc tgctcgacat tcacgcgaat tgaggagcgc cgacgatgcg
      901 gcattggatg cgcaagctcg tgaccggcgc gggcctcgca tggctcgcgc tttcgccggt
      961 ggctgcgcgt gccgattccg taagcgattt ctatcacggc cggcagatga tcatgatcct
     1021 gtcggccgat gcgggcggcg gctatggcag ttacgccaat gccttcgcgc cctatctggc
     1081 gcagcacatt ccgggcaagc cccgcatcgt cgtgcaatac atgccggggg cggggggctt
     1141 gcgcgcgatg aattatctct attcggcggc ccccaaggac ggcagcacaa tcgcgctcgt
     1201 gcattcgagc gtgcctttcg cgccgcttta cggtttggcg gcggcgaaat tcgatccgcg
     1261 caagatcaac tggatcggct cggtcaacgc gacgtcgggc atctgcgtcg cgtgggcgac
     1321 atcgaacatc aaaagctgga acgatctttt cacgaagcaa ttcgtggtgg gaagttcggg
     1381 cgccggctcg cagatggaaa ccattcccgc gatgttgaac aaattgttcg gtaccaagat
     1441 caaagtgatc tcaggctatc gcggcgggaa cgaagtctat ctcgcgatgg aacgcggcga
     1501 ggtgcagggc cgttgcggcg ggctgatttc ctcgatcaat tcgacgcgcc ccgattggtt
     1561 cccgcagaag aaggtcgcgg tgccgatcca aatcgcgctc gaacgcgatc cccaattccc
     1621 cgattctccg gcgctgatcg aattcgccaa ggacgcgcgc acgaaacaga tccttcagct
     1681 cgtgctggcg cccatggcga tggaccgccc catcctcacg ccgccgggcg tgcccgcgga
     1741 ccgggttgcc gccttgaagg ccgcgtttca tgcggccgtc aacgatccgg cgtttctggc
     1801 cgatgccaag aagcagcgcc ttgaggtgaa agaggtcagc ggcgaaaagg tcgcggggct
     1861 tctcgccaca gccttcgcca tgccgccgga cgtggtgaaa gcggccaacg atgccatggg
     1921 cggcgcggcc ggccggggag aataagggtc gaagataagg ggagagggat catgaagcgt
     1981 ttcatattcg cattgcttgc cggcctcgcg cttggcgcat cgtctggagc cttggcgcag
     2041 ccctcgtcgg tcaagccgat gacctttccg gtggtgcagg gcaagaccta caaattcgag
     2101 aagatcgccg acggcgtcta ttacgcgacc ggcggtttcg gcagcaacaa tgtcgtcatc
     2161 gtcaacgaca atgacgtgat gctggtcgac accggcacca cgccgggcaa tgcgcgcaga
     2221 ttcgttgccg acgtgaagat gctcaccgac aagccgatcc gctatgtggt gaacacgcac
     2281 tggcatttcg atcacacgga cggcaaccag atcttcggcc ctgacgtgca gatcattggc
     2341 caggaatatc tcgccaaggc aatcacgccc gacatcgtga atcgcgagcc cttccgcacc
     2401 tccaccggcc cggtgatcca ggcgcagatg gacatgttga aaaagcagat cgcggacgag
     2461 aaagacaccg ccaagaaggc ggcgctgaac aagcagctcg ccgacgatca gcttctgcaa
     2521 tcggagctga aggagctgaa accgacgccg ccgaataaga cgtataagga caaactcgtt
     2581 atccagagcg gggcgcgtga gatcgatctc ctgtttctgg gacgcggtca caccgaaggc
     2641 gacacggtgg tctatctgcc caaggaaaag atcgttgcga ccggcgatct gatggaaagc
     2701 cgcatcgcct atatgggcga tgccgtgttc gacgaatgga tcgacacgct ggacaagctc
     2761 aagaagctcg acttcaacgt cgatcttccc ggtcacggaa tcgcattctc caacaagggc
     2821 ctgatcaccg cgttccagga ttacatccag gacctgatcg taaagggcaa tgagctgaag
     2881 cgcgccggca tgtcggcgga cgacatggcc aagacgatcg acttgacctc gcatgcaaag
     2941 gattttccgc agatcaccgg cccgggcatg gaccttcgcg gcgcgcgccg gctttacgcg
     3001 tggctgaccg aacgcggcgg cggctgagaa cgcggaccga agcgcgcccg tggcttcggc
     3061 gaggaaaatc atcatcacct gcgcggtgac gggggcgatc cacacgccct cgatgtcgcc
     3121 gcatctgccc atcacgcccg atcagatcgt cgccgatgcg ctgg
//
LOCUS       DASYEG010000244         7225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1973662, whole genome shotgun sequence.
ACCESSION   DASYEG010000244 DASYEG010000000
VERSION     DASYEG010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7225
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1973662"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..340
                     /locus_tag="VGM72_10510"
                     /pseudo
     CDS             103..340
                     /locus_tag="VGM72_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611759.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            424..1578
                     /locus_tag="VGM72_10515"
     CDS             424..1578
                     /locus_tag="VGM72_10515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY4265741.1"
                     /translation="MASREEQVAIREAIRKICADFPDTYWRDIDHAGAYPQAFVDALT
                     ASGYLAVLIPEEYGGAGLGIAEAGIILEEINRSPGNGLACHAQMYTMGTLLRHGSQEQ
                     KRRYLPDIASGALRLQAFAVTEPNAGSESTRIETRAVRDGDKYIVNGQKIFISRVLQS
                     DLMLLLARTTPYDALTDKTQGLSVFLVDLRDLKGKFEVRPLDMMFNHHTNILFFENVE
                     IPVENRIGEEGKGFRYIIDGWNAERILIASEAIGDGRWFVERGSKYASERVVFGKPIG
                     TNQGVQFPLARAYAHVEAAACMRDVAAEKFDSNEKCGAEANMAKLLSSEAAWEAANAC
                     LDAHGGYGFAREYDVERKFRETRLYMTAPINNNLVLAFLGQNVLGMPKSY"
     gene            1597..2514
                     /locus_tag="VGM72_10520"
     CDS             1597..2514
                     /locus_tag="VGM72_10520"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEY4265742.1"
                     /translation="MNEHAKRLYRSWMFVPGDRQRMIDKALDSNVDAIMMDIEDGVAP
                     SEKDNARRQIGAALDGVCARLKSEPAYRTPGRYVRVNAVGTERFIQDTNAVIRPGLEG
                     LVVSKVESVAQIRIAEDELGRAEVAHHMPGGTVKLLIAIESPIGLFNAYTIASSSPRI
                     VGLLFGAEDFTREMNLPMRREGEAMDMIYARSHLATAAAAAHVQAVDGVWVDLNDMDG
                     MRRFAKQARRLGMSGISIIHPNQIDDANAAFTPTKEDIDYAQAVVNAFEDARAKGQGA
                     IAFRGQLLDYPIVDRARQTLTLAKTLGVA"
     gene            2527..3027
                     /locus_tag="VGM72_10525"
     CDS             2527..3027
                     /locus_tag="VGM72_10525"
                     /inference="COORDINATES: protein motif:HMM:NF039588.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY4265743.1"
                     /translation="MAVKPGWEGRYFEDFEIGDVYRCRLGRTVTEADNIWFTLLTNNT
                     NQIHFNNEFGKKTEFGKCLINSALTLAIVAGMGVADVSENGFALGWDGITLPNPLFAG
                     DTLYSESEVLETRESKSKPQWGIVKVRTRGLQQEGKIVIDYVRSVMVWKKAHAPHRDI
                     FPEVKS"
     gene            3024..4229
                     /locus_tag="VGM72_10530"
     CDS             3024..4229
                     /locus_tag="VGM72_10530"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY4265744.1"
                     /translation="MTGDDRTRPRRALDGVRVLALETSLSGPHATKILADMGAEVIKI
                     ERPGAGDVIRGWDDAVDGLSSGIVWIGPNKKSFTIDVKKPAAREILQRLAARVDVVLE
                     NFAPGAAARMGLGAEELRSANPRLIYCSLSGYGQDGPYRDVKAYDALIQGEAGLIMTT
                     GFEDRPARVGVSVTDLISSMYAAVGILTALYQREQTGVGQVIDISMFETAASWLGYFP
                     HHYWHRGEEPKRYGLRHQYITPYGHYLASDGRYFGLAVASPADWEIFCNAVIERPDLL
                     ADARFKDTPARRANREELDEILIGIFMTRPSAEWLARLDRVRLPHGTVNGVGDVLAHP
                     QMKAREMIQEIDSPVGRIPVLASPLRLSDSPQRLDPMPALGGDTASILSGLGYSALEI
                     TAFRKDGVI"
     gene            complement(4222..4893)
                     /gene="npdG"
                     /locus_tag="VGM72_10535"
     CDS             complement(4222..4893)
                     /gene="npdG"
                     /locus_tag="VGM72_10535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEY4265745.1"
                     /translation="MTTEKIAIIGGAGELGFGLALRWAKAGEDVTIGSRDAAKAAEAA
                     ARVGAAVPGARIAGLGNVQAAGLATIAVLAVPFAAQAGILKSIKPALKNAILVDTTVP
                     LAASVGGRASRVLGVWEGSAAQQAAGLVPGVPVVSAFHNVSAEMLHDPNLTLDCDILI
                     SGDDASAKERLSALVRLIPGLRPLDAGPLEMSRVVESITALMISLNRRYKTHYGGIRI
                     TGLAI"
     gene            4986..5741
                     /gene="cofE"
                     /locus_tag="VGM72_10540"
     CDS             4986..5741
                     /gene="cofE"
                     /locus_tag="VGM72_10540"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEY4265746.1"
                     /translation="MTSPSVQIFPLPGLPEISPGDDLARLIAAVLRVGDLQAEAGDVF
                     VIAQKIVSKAEGRVVRLDTVTPSERAVAWAKAWDKDARAVELVLRESRRIVRMERGII
                     IAETRHGFVCANAGVDLSNATSGTAILLPENPDESARMLQAQLAAVLGVDVAIIISDT
                     FGRPWREGLVNVALGVAGIAALLDYRGETDAGGKLLQATIIAIADELASAAELVMGKA
                     DRVPVAIIRGVRAASREGSGRDLIRPADRDLFR"
     gene            complement(5748..6365)
                     /gene="cofC"
                     /locus_tag="VGM72_10545"
     CDS             complement(5748..6365)
                     /gene="cofC"
                     /locus_tag="VGM72_10545"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEY4265747.1"
                     /translation="MKSFAQSKTRLAAHFSPQARAALAAALCADFFAMIAGVARANRV
                     FVVSREPQALERARALGWETIVETEQISESHSVDAASRICAAQGVSALLRIPMDLPLV
                     TAADIDEILAEAANAPSCVIVPSFDGTGTNALLRSPPSLFASHFGPDSFARHLEEAER
                     CGARVKILHNAHIARDVDEWDDLRALLGHTRASSETARWISQHLT"
     gene            complement(6383..>7225)
                     /gene="cofD"
                     /locus_tag="VGM72_10550"
     CDS             complement(6383..>7225)
                     /gene="cofD"
                     /locus_tag="VGM72_10550"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEY4265748.1"
                     /translation="ANTGDDIVMHGLHVSPDPDILIYTLAGVVNPQTGWGFRDETFRV
                     AEGLAGYGRAVWFQLGDRDLATHIHRTAMLKEGATLSEVIESIRSAHGVGTRILPMSD
                     SPVPTMLQTGEGRMHLQEYFVRRRCEPRLEAIVFEGAERALAAPGVIDCLHASDAIVI
                     APSNPLISIGPILAVPGIRDALRENRARVIAVSPLVGGKSLKGPSDKMMREMGHEVSA
                     AGVARLYRDICANFVIDETDAEQSPAIAALGMTPIVCPTVMRSTADKEQLARVVLRLG
                     GARA"
BASE COUNT         1400 a         2268 c         2272 g         1285 t
ORIGIN      
        1 ccacttcgat gtgtatggga aaaggacgga tgaagcgatg aaaccggtcc tcgaatggtt
       61 caaacagtac cttcaatgaa tcaacgcagg cgctggtgac tttcggtcgt aaagaccgcc
      121 tgcatcgaac cgggaagccc gtgggagaac ggctactgcg aaagcttcaa cggatgaact
      181 cctcaacgga gaaatcttct attccgtgaa ggaagcgcaa atcatcatcg agcagtggcg
      241 tcgcttctac aacacaaagc gcccgcaccc ggccctgggt taccgcccgc cctcgccgga
      301 atccttcatc ccgttgttgg gaacgatgcc catgcactaa gattcaaact ggaccggtcg
      361 cagggggctg atcagtgcgt ctatttgaac caacaaaata gactgacgta taggagttag
      421 attttggcat cgcgcgaaga acaagtggcc atccgggaag ccatccgcaa gatctgcgcg
      481 gacttcccgg atacctattg gcgcgacatc gatcacgccg gcgcctatcc gcaagccttc
      541 gtcgatgcgc tgacggcctc gggctatctc gcggtcctca tcccggaaga atatggcggc
      601 gcggggctcg gcatcgccga agccggcatc atcctggagg agatcaatcg ctcgccgggc
      661 aacgggctcg cctgccacgc ccagatgtac accatgggca cgctgctgcg gcatggcagc
      721 caagagcaga agcggcgcta tctgccggac atcgcgagtg gcgcgcttcg gttgcaggcg
      781 ttcgcggtca ccgaacccaa tgcgggatcg gaatccaccc gcatcgaaac gcgcgcggtg
      841 cgcgatggcg acaaatacat cgtcaacggc cagaagatct tcatcagccg tgtgctgcaa
      901 tccgatttga tgctgctgct cgcgcgcacc acgccttacg atgcgctgac ggacaagacg
      961 caagggctgt cggtgttcct cgtggatttg cgcgacctca agggcaagtt cgaggtccgc
     1021 ccgctcgaca tgatgttcaa tcaccatacc aatatcctgt tcttcgagaa cgtcgaaatc
     1081 ccggtggaaa accgcatcgg cgaggagggc aagggctttc gctacatcat cgacggctgg
     1141 aacgccgagc gcatcctgat tgcctccgag gcgatcggcg atggccgctg gttcgtcgaa
     1201 cgcggcagca aatatgcgag cgagcgcgtg gtgttcggca aacccatcgg caccaatcaa
     1261 ggggtgcagt ttccgctcgc gcgcgcctat gcccatgtcg aagcggcggc ctgcatgcgc
     1321 gatgtggcgg cggaaaaatt cgattcgaac gagaaatgcg gcgccgaggc caatatggcg
     1381 aagctgcttt ccagcgaggc ggcgtgggag gcggccaacg cgtgtctgga cgcgcatggc
     1441 ggctatggtt tcgcgcgcga atatgatgtg gagcgcaaat tccgcgagac gcggctttat
     1501 atgaccgcgc caatcaacaa caatctggta ctggcgttcc tcggccagaa cgtgctgggc
     1561 atgccgaaat cctattgaac gcgacgaggc tttttcatga atgaacacgc caaacgactg
     1621 tatcgctcct ggatgttcgt gccgggcgac cgccagcgca tgatcgacaa ggcgctcgac
     1681 agcaatgtcg atgccatcat gatggacatc gaagatggtg tcgcgccgtc ggaaaaagac
     1741 aacgcccgcc ggcagatcgg ggccgcgctc gacggcgtct gcgcgcggtt gaagagcgag
     1801 cccgcctatc gcacgcccgg gcgctatgtc cgcgtgaatg ccgtgggaac ggagcgtttc
     1861 atccaggaca cgaatgcggt gattcgccca ggcctcgaag ggctggtggt gtcgaaggtg
     1921 gaaagcgtcg cgcagatccg cattgctgag gacgaacttg gccgcgcgga ggtcgcccat
     1981 catatgccgg gcggcacagt gaagcttctg atcgccatcg aaagcccgat cggattgttc
     2041 aacgcgtaca ccattgcttc gtcatcgccg cgcatcgtgg gcctgctgtt tggggcagag
     2101 gatttcacgc gcgagatgaa tctgccgatg cggcgcgagg gcgaagcgat ggacatgatc
     2161 tacgcgcgtt cgcatctcgc gacagccgcg gctgccgctc atgtgcaagc tgtggacggg
     2221 gtctgggtcg acctcaacga catggatggg atgagacgtt tcgccaaaca ggcacgccgt
     2281 ctcggcatga gcgggatttc gatcattcat ccaaaccaga tcgatgacgc gaacgcggcc
     2341 ttcaccccca cgaaagagga catcgactac gcccaggccg tggtgaacgc gttcgaggat
     2401 gcgcgcgcga aggggcaggg cgccatcgcc tttcgcggcc aattgctgga ttatcccatc
     2461 gtggatcgtg cccgccagac gctgaccctc gccaagacgc tgggcgtggc ctgagaggag
     2521 gaatctatgg ccgtcaaacc gggttgggag gggcgctatt tcgaggattt cgagatcggc
     2581 gacgtttatc ggtgccgtct ggggcgcacg gtgaccgagg ccgacaatat ctggttcacg
     2641 ctgctcacca acaacaccaa tcagatccat ttcaacaatg agttcggcaa gaagaccgaa
     2701 ttcggcaaat gcctgatcaa ttccgcgctc acgctggcca tcgtcgcggg catgggtgtc
     2761 gccgatgtca gcgagaacgg cttcgcgctc ggctgggacg gcatcacgct gcccaatccg
     2821 ctgttcgcgg gcgacacgct ctattcggaa tccgaagtgc tggaaacgcg cgaatccaaa
     2881 tccaagccgc aatggggcat cgtcaaggtg cgaacgcgag ggcttcagca ggaaggcaaa
     2941 atcgtcatcg actatgtgcg cagcgtcatg gtgtggaaga aagcccatgc gccccatcgc
     3001 gacattttcc ccgaagtgaa atcgtgacgg gggatgatcg gacacgccca cggcgcgcgc
     3061 tggacggcgt gcgcgtgctg gcgctggaga cctcgctgtc gggtccgcac gcgaccaaaa
     3121 tccttgccga catgggtgcg gaggttatca agatcgaacg ccccggcgcg ggcgatgtga
     3181 tccgcggctg ggacgatgcg gtagacgggt tgtcctccgg catcgtctgg atcggcccca
     3241 acaagaaaag cttcaccatc gacgtgaaga aacccgccgc gcgggaaatt cttcagcgtc
     3301 ttgcggcccg tgtcgatgtc gtgctggaaa atttcgcgcc gggcgccgcc gcccgcatgg
     3361 gcctgggcgc ggaggaattg cgcagcgcca atccgcgcct catttattgc tcgctctccg
     3421 gctatgggca ggacggcccc tatcgcgacg tgaaagccta tgacgcactg atccagggtg
     3481 aagccggcct catcatgacg accggattcg aggatcgtcc ggcgcgcgtc ggcgtttcgg
     3541 taaccgacct catctcgtcc atgtatgcgg ccgtcggcat cctgaccgcg ctctatcagc
     3601 gcgaacagac gggcgttggc caggtgatcg acatttccat gttcgagacc gcggcgtcct
     3661 ggctcggcta ttttccgcat cattactggc atcgcggcga ggaacccaaa cgctacggcc
     3721 tgcgccacca atacatcacg ccctacggac attacctcgc gagcgatggg cggtatttcg
     3781 gcctcgccgt ggcgagcccc gccgattggg aaattttctg caacgccgtc atcgagcggc
     3841 ccgatcttct cgccgatgcg cgcttcaagg acacgccggc gcgccgggca aaccgcgagg
     3901 aactggatga aatcctgatc gggatattca tgacgcgccc gtccgccgag tggctggcgc
     3961 ggctggacag ggtgcgcctg ccgcacggca cggtgaatgg tgtgggcgat gtgctggcgc
     4021 atccgcagat gaaggcgcgt gagatgatcc aggaaatcga ctcgccggtc gggcgcatcc
     4081 cggtgctcgc cagccccttg cgtctttccg acagtccgca gcggctcgat cccatgcccg
     4141 ccctcggcgg cgatacggct tcgattctca gtggtcttgg ctatagcgcg ttggaaatta
     4201 ccgcgttccg caaggacggg gttatatagc caaacccgtg atccggattc cgccgtaatg
     4261 cgtcttgtag cggcggttga gcgaaatcat cagcgccgtg atgctttcca ccacgcgcga
     4321 catttcgagc gggccggcat ccagcggccg caagccggga ataagcctca ccagtgccga
     4381 cagccgttcc ttggcgctcg catcgtcgcc ggaaatcagg atgtcgcaat cgagcgtcaa
     4441 gttcggatcg tgcagcatct cggcggagac attgtggaac gccgaaacca ccggcacgcc
     4501 cggcacgagc cccgcggcct gttgcgcggc cgagccctcc cacacgccca gcacgcgcga
     4561 cgcccgcccg ccgacacttg ccgccaatgg cacggtcgta tcgaccagaa tggcgttctt
     4621 cagcgcgggc ttgatgctct tcagaattcc cgcctgagcc gcgaacggca cggccagcac
     4681 ggcgatggtg gcaagccccg ccgcctgaac attgccgagg cccgcgattc tggcgcccgg
     4741 aaccgcagca cccacgcgcg cggcggcctc tgccgccttt gccgcatcgc gcgagccgat
     4801 ggtcacgtcc tcgccggcct tggcccagcg cagcgccagc ccgaagccca gttcaccagc
     4861 gccgccgatg atggcgattt tttccgtcgt catgcgcatc tcccttgccg gtggttgtgt
     4921 cacgctgttg tgcccgccgc aagaatttgc ggctattgtt cacgatcctt cgaaagtgtc
     4981 agcgtgtgac ctcaccatcc gtacaaattt tccccttgcc gggcttgccc gaaatcagcc
     5041 cgggcgacga tctggcgcga ctaatcgcag ccgttctcag ggttggcgat ttgcaggccg
     5101 aagcgggcga tgtgttcgtc atcgcgcaga agatcgtctc caaggccgag gggcgcgtgg
     5161 ttcggctcga tactgtgact ccgtccgaac gcgccgtggc ctgggcgaaa gcctgggaca
     5221 aggatgcgcg cgccgtcgag ctggttctgc gcgaatcccg ccgcatcgta cgcatggaac
     5281 gcggaatcat catcgcggag acgcgccacg gtttcgtctg cgccaatgcc ggcgtcgatc
     5341 tctccaacgc gacatccggc acggcaatcc tgctgccgga aaatcccgat gaatcggcgc
     5401 ggatgctgca ggcgcaactc gcggcggtgc tcggcgtgga tgtcgcaatc atcatctccg
     5461 ataccttcgg ccgcccctgg cgcgagggcc tcgtcaatgt cgcgctcggc gtcgccggca
     5521 tcgcggcgct cctcgattat cgcggagaga ccgacgcggg agggaaattg ctccaggcaa
     5581 cgatcatcgc tatcgccgac gagctcgcct ccgccgcgga gctggtgatg ggcaaagccg
     5641 accgcgtgcc ggtcgccatt atccgcggcg tgcgggcagc ctcacgcgaa ggcagcgggc
     5701 gcgatcttat ccgccccgcg gacagggacc tgttccgctg aggcggttca cgtcagatgc
     5761 tgcgagatcc atcgcgcggt ctcggaactg gcgcgggtgt gtcccagcaa ggcgcgaaga
     5821 tcgtcccatt catcgacgtc gcgcgcgata tgcgcgttgt gcaggatctt gacccgcgca
     5881 ccgcagcgtt cggcttcttc cagatgccgc gcgaaactgt cgggaccgaa atgcgatgcg
     5941 aacaaactgg gcggcgaacg cagcagcgcg ttcgtgccgg ttccatcgaa cgaaggaacg
     6001 atgacgcaag atggagcatt tgcagcttcc gcgagaatct cgtcgatgtc ggctgctgtc
     6061 accaaaggca aatccatcgg gatgcgcaaa agcgcggaga caccttgcgc ggcacagatg
     6121 cgcgaggccg catcgaccga atgactttcg gaaatctgct cggtttccac gatggtttcc
     6181 catcccaatg cgcgtgcgcg ctccagcgct tgaggctcgc ggctgaccac gaacacgcga
     6241 ttcgcgcggg cgacacccgc tatcatcgcg aaaaaatccg cgcacagcgc ggcggcaagt
     6301 gccgcgcgcg cctggggcga gaaatgcgcc gcgaggcgcg tcttcgattg cgcgaacgac
     6361 ttcaccggga tcaaaacgcc cctcacgcgc gcgcgccgcc aagccgcagg accacgcggg
     6421 caagctgctc cttgtcggca gttgagcgca tcacggtagg acagacgatt ggcgtcatcc
     6481 caagggccgc gatggcggga ctctgttccg catcggtttc gtcgatgacg aaattcgcgc
     6541 agatgtcgcg atagagccgc gcaacgccgg ccgccgacac ctcatgtccc atttcgcgca
     6601 tcattttgtc ggatggccct ttgagcgatt ttccgccgac cagcggcgag accgcgatga
     6661 cgcgcgcacg gttctcccga agcgcgtcgc gaatgccggg cacggcgagg atggggccga
     6721 tgctgatcag cggattgctc ggcgcgatca cgatagcgtc cgacgcatgc aggcaatcga
     6781 tgacgcctgg ggcggcaagc gcgcgctcgg caccttcgaa gacaatcgcc tccagtcgag
     6841 gttcgcagcg ccgccgcacg aaatattcct gcagatgcat gcgtccctcg ccggtctgga
     6901 gcatggtcgg cacgggagaa tcgctcatcg gcagaatgcg tgtgcccacg ccatgcgcgc
     6961 tgcggatcga ctcgatcacc tcggacagcg tcgcgccctc cttcagcatg gcggtgcgat
     7021 ggatatgagt cgccaggtcg cggtcgccaa gctggaacca cacggcacgc ccgtaacccg
     7081 ccagcccctc ggccacgcga aacgtctcgt cgcgaaagcc ccaaccggtt tgtgggttca
     7141 cgacgcccgc cagcgtgtag atgaggatgt cgggatccgg cgaaacatgc agcccgtgca
     7201 tcacgatgtc gtcgccggtg ttggc
//
LOCUS       DASYEG010000245        16743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1101473, whole genome shotgun sequence.
ACCESSION   DASYEG010000245 DASYEG010000000
VERSION     DASYEG010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 16743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16743
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101473"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VGM72_10555"
     CDS             complement(<1..353)
                     /locus_tag="VGM72_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265749.1"
                     /translation="MFETEAAWLEMLLRERTPDDLSPLLNVGSSTSDFREVAQPWAER
                     RLFAPLRARGVQIIHLDTREGHGIDIKADILSDADLPRIRSFKPRSILCCNILEHVRD
                     PKALARRCAEIVGPGG"
     gene            512..1396
                     /locus_tag="VGM72_10560"
     CDS             512..1396
                     /locus_tag="VGM72_10560"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEY4265750.1"
                     /translation="MRNESGTLEDLRGEIDRIDDALHDLLIRRADVSLAIAKVKQPVP
                     GGAGAFIPALRPAREAAILRRLLARHKGDLPPQVLVRIWREIIAASLRVQSKFQLHIY
                     ASDNHSGLADLAHAHFGSQTPLRTHTRSSMVVHACAEEPHSLGIVPLPEIEEAGPAWW
                     AQLAPAGEKGPRVVAKLPFVQDGEDGLSAYAIGTVEQEPSGDDTTLLLLEIAPGVSRA
                     TLQSFLKSAGLNAKLTAAGRLREKNAPDEILLEVKEFVGKDDPRLTALADAAGGAIIR
                     AAPIGGYANPVMLPAAVR"
     gene            1410..2501
                     /gene="hisC"
                     /locus_tag="VGM72_10565"
     CDS             1410..2501
                     /gene="hisC"
                     /locus_tag="VGM72_10565"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY4265751.1"
                     /translation="MTPSPKPGVMDIDAYVPGRSEAKGAARVYKLSSNESPFGPSPKA
                     IAAYEAAEPVLGVYPEGSARILREAIAAHYGLAADRIVCGNGSDEILTLLANCYVRPK
                     DEVLFGAHAFSLYKIVTLANSGVPVEVPTRDLQLDVGDVIARVREKTRLVYIAHPNNP
                     TATYIPVSEMRRLHRALPDSALLVIDAAYAEYVQKNDYEAGIELVSTTNNVVMTRTFS
                     KVYGLAGIRIGWAYCPKPVADVLNRVRAPFNINIAAQRAAVAALADRGHTENAIQHNA
                     FWRSWLTEQIRGLGLRADDSAANFLLIHFPDTPGRSADDADRFLMARGIIMRACGSYT
                     LPQCLRLTVGSEEANRAAIAALDEFMASQ"
     gene            2498..3436
                     /locus_tag="VGM72_10570"
     CDS             2498..3436
                     /locus_tag="VGM72_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate/arogenate dehydrogenase family
                     protein"
                     /protein_id="HEY4265752.1"
                     /translation="MNDTSPIFEKIALIGVGLIGSSIAHAARRGGLARHIAAYVPRAE
                     TRARVEKIGFADSLHDQLESAVAGADLVVLATPVGAYAALAASIAPYLKPGAILTDVG
                     SVKGQVIKEIGPHVPKDVHFIPGHPVAGTEQSGPEAGFAELFDGRWCILTPPAGTDPE
                     AVERLKAFWQRCGSAVETMDAKHHDLVLAITSHVPHLIAYNIVGTAADMETVIQSEVI
                     KYSASGFRDFTRIAASDPTMWRDIFLNNREAVLEMLGRFNEDLAALQRAVRWGDGDAL
                     FNLFTRTRAIRRSVIEAGQDTPAPDFGRSGNVAGGR"
     gene            complement(3410..4363)
                     /locus_tag="VGM72_10575"
     CDS             complement(3410..4363)
                     /locus_tag="VGM72_10575"
                     /inference="COORDINATES: protein motif:HMM:NF021423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2125 domain-containing protein"
                     /protein_id="HEY4265753.1"
                     /translation="MSYSNRIFFYGPVSLLLLVIVLYSVFWRVQADTLAARLDRANGG
                     EVIPGIVFAFAEKSVGGYPFRIDAVLSGVTFSHQAPEGETAWRTEKLALHRLSYGQNR
                     YIFEASGLQSFAQPPLTRGTVPRVTFVTPGIARASAILVNDRLTRFDLDLLEPQVKDA
                     TRGAEPNRTAVAARAQFHLLARPDDSIDLALMIDNAKIGNGYAGGMDETQLTRIDLRA
                     KFTQGDALDALRAGMMSLADAAAAWRGRTGTLTVNNLALDWPGGHADLKGDLALDEND
                     RVTGALQGDGPKGKTATSFGLAFANGDMRFSSGSAASGHVP"
     gene            complement(4403..6775)
                     /locus_tag="VGM72_10580"
     CDS             complement(4403..6775)
                     /locus_tag="VGM72_10580"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEY4265754.1"
                     /translation="MDALSHQIWDMKYRLKAADGTPLDRSFEESWARVARAAAEAEAP
                     EKRHEWAAAFEASLAGHHFLPAGRILAGAGTGRSVTLFNCFVMGRVPDDLSGIFAHLR
                     EAALTMQQGGGIGYDFSTLRPKGAPVKGVGADASGPVSFMDVWDSMCRTIMSAGARRG
                     AMMGTLLCSHPDIEDFIAAKRAGNRLRNFNLSVLVTDAFLRAVETDDSWDLTFDGTVY
                     RTLSARALWDKIMITTYEAAEPGVIFIDRVNALNNLSYCETIYATNPCGEQPLPPYGA
                     CLLGSINLAKLVDHPFTEDARLDETKLARLARVAVRFLDNIIDVSRFPLPAQEDEAKQ
                     KRRIGLGVTGLADALIFARARYGSSRSLALIEHWLRIVRDEAYGASAELAAEKGAFAL
                     FDKDAYLARPNIAGLPEPIRAAIAGHGIRNGLLTSIAPTGTISLFADNISSGIEPVFA
                     HSYTRNVLMPDGTRREEKVSDYAYRRFRGLFGEDTQLPDYFVTAQELSPSEHVAVQAA
                     AQKYIDSSISKTINCPPQISFADFKDVYRTAYREGCKGCTTYRPNPVTGAVLETERAE
                     GLAPSAVLPANLAPAVQAVPSIAARGARALPGTAPAYRFAERAEPADVVYMTRPLDRP
                     GDLPGHTYKVRWPDLDHAFYITINDIEEGGRKRPFEIFINSKNMEHYAWTVALTRMIS
                     AVFRRGGDVSFVVEELKSVFDPRGGQWMGGRYIPSLLAAIGEVIERHLIAIGFMTQPG
                     SGESDAQRAAAAVAGAEGAAVRTCPRCSAAGFVKLEGCDTCISCGYSKCT"
     gene            complement(6929..7408)
                     /locus_tag="VGM72_10585"
     CDS             complement(6929..7408)
                     /locus_tag="VGM72_10585"
                     /inference="COORDINATES: protein motif:HMM:NF022214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 family protein"
                     /protein_id="HEY4265755.1"
                     /translation="MSSRDAVVTQETRLQGVRGWLLALCLYLTLLIPLLAVLGLVGAW
                     QAAARSATLQNALVYEAVLELGLAGFAFYSGIALYRRRPNATSIAKIYFITMLTLGVL
                     GLGIALVGAVWQLSDRALANQLRGPAVVAGLRQIVLAVAWLLYLERSRRVHATYPAG"
     gene            complement(7433..8173)
                     /locus_tag="VGM72_10590"
     CDS             complement(7433..8173)
                     /locus_tag="VGM72_10590"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY4265756.1"
                     /translation="MILIVRSALFLAWFISISVIMNVGLLPLLLLPPGATLAAARIWA
                     KLSLFGLKWIAGTGLEVRGTFAEPRVLVASKHFSMWETIAFLCLLPGPAIVIKQSLLR
                     IPLYGWYCRKMRMIAIDRSAGAKAIRAMAAEARRASNEGRPIVIFPEGTRKKPGDAPD
                     YKPGVAALYALLDIACVPAAHNSGLFWTGFLKRPGRIVVEFLEPIPPGLTRQAFMDEL
                     QRRTEDATNRLLAEAGREANSRSTAPGL"
     gene            complement(8170..8808)
                     /locus_tag="VGM72_10595"
     CDS             complement(8170..8808)
                     /locus_tag="VGM72_10595"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEY4265757.1"
                     /translation="MKQREHGQTHASGRTKRKRTLRTALSLAVLALVLYAIGFSFFVV
                     DLPLPRAGAIAKADAIVALTGEGGRLGPAVTLLEAGGGQRLLITGVNKLISKPTLKTL
                     LHGGQAFDCCADLGFAALDTRGNAAEAARWVRAHRYGSLIVVTATYHMPRSLVEFGAE
                     MPGVTLIPYPVSAEAVKMLSWENAKRLNGEYVKYLASIVRVCVAGALGRRDA"
     gene            complement(8827..9735)
                     /locus_tag="VGM72_10600"
     CDS             complement(8827..9735)
                     /locus_tag="VGM72_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265758.1"
                     /translation="MSESATREPRQRRAGRILPREDGAISLDLVIAVMAFLAGLALGG
                     VLIAQRTAESWQAGLAGRITVQILPEGDSAPAREVNAAVRILSGTPGILHATALSDAD
                     NLALVEPWLGRDAVVAVLPFPRMIDVQLAAGATPDLTALEKRLKAAAPHAVLDDHGRW
                     IGRLKNTANSVVFAALAILALIAVGTAATVAFATRAGLAAHHEIVQLLHLMGAQDRFI
                     ARAFEWHYFVAALIAAACGAAIATLIFAAAGSLDQLGVSSVSFLPPLGLPLRDLGWLA
                     LIPAGAAAIAWATARLSVVAALHELY"
     gene            complement(9732..10400)
                     /gene="ftsE"
                     /locus_tag="VGM72_10605"
     CDS             complement(9732..10400)
                     /gene="ftsE"
                     /locus_tag="VGM72_10605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEY4265759.1"
                     /translation="MVRFENVGMRYGAGPEVLRDVSFALDPGSLTFLTGPSGAGKSSL
                     LKLVFMAEKPSRGLISLFGRDLARLKRADLPALRRRVGVVFQEFRLLDHLSAFDNVAL
                     PLRLAGHRPAEYRRDVEELLSWVGLGTRLQARPPTLSGGEQQRVAIARAVVAKPDLLL
                     ADEPTGNVDPEMGERLIRLFAELNRFGTTVMIATHDRALVESTRAREMRLIDGRVIEM
                     RSNR"
     gene            10583..11323
                     /locus_tag="VGM72_10610"
     CDS             10583..11323
                     /locus_tag="VGM72_10610"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HEY4265760.1"
                     /translation="MILTCPSCRTRYQTDGAQFKPPGRNVRCAKCGQVWYQAKPEPET
                     EVEPEPVLAPPSVPPPSKPMAAELAAPMHGALDFGVASGIEPEPASADIRLRSRGTAF
                     AQAVGWLALIGLIGAIGWSTIQYRETIAGLWPQSASLYSVLGLPVNVRGITLTNIAYK
                     QEYEDGQQVLSVTGKIVNVSKRDQVVPEIRVVLLDNAKHELYHWTFDAGIQTLKPGTE
                     TPFMTRLSSPPAEARDLNVRFVDSGETR"
     gene            11352..11903
                     /locus_tag="VGM72_10615"
     CDS             11352..11903
                     /locus_tag="VGM72_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEY4265761.1"
                     /translation="MMGAEAQERILFSEEQIASRVKEVAREIAGAPLRPGIAVPILAG
                     AFVFAADLLRALASEGLDLETEFIWLRSYGASDTPSEIAVLKGPGDLVRGRTVLMIDG
                     VLDRGVTCARARQLLMAAGAVQVICAVAVVKSYPDARYRADYALFRAGEEFLYGYGMD
                     RASLSRGLPDIRVTENKKLSRPA"
     gene            complement(11916..14570)
                     /locus_tag="VGM72_10620"
     CDS             complement(11916..14570)
                     /locus_tag="VGM72_10620"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02302 family protein"
                     /protein_id="HEY4265762.1"
                     /translation="MSTENKNEREARRQKAERFVATARLILLWERIWPAMWPGLGIFG
                     AGMILALTGAFAFIPGAVHAVLLLAFFGALLFVFWRSFARFRNPRWEDGARRVERDSD
                     LPNRPITEGMDVVAAGKGDPLAERLWRAHIIRLLASAKGLRLALPSPGMGRRDPRGLR
                     FAVLLGVLAGFLIAGPQSGQRLVSGLFPVFGDAVDNSVFVAWVSPPIYTGLPPRSLTD
                     TTVMTASGDIQAPVNSTLVMRLRGTSSRPTIDARPIPKGGQPKFVKGDAGYEAKLNIA
                     VNSHVSIRLGSHSYGNYYFITIPDKPPTIAFTETPAAQQNAALKLSYKGQDDYGVVKA
                     AARIRPLDDKGREIASAETLTVELPVPGTGRVVSDTVYRDLTAHAFAGSNVHISLQAT
                     DAAGQTGTSAPLTIKLPQRVFTEPLAQSLIEQRKTLAVDGEKSRPKVQTALDALSLGP
                     QQFYKDDFSNYLALRSLNYRLSSPKNDPGFKTSQNFMWDMALAIEDGDLANAAEELRK
                     VQEQLQDALERGAPDEEIRALMDKLRQAMARYMQQLARNAQRGNQQMGQMPPGARAMS
                     QKDLQDLLKAIEDLARTGNRDQARQMLAALMQMMENMKMMAGRGGQPGQPGQQGQQGQ
                     GTPQENAMNQALQGLGDIMGGQRQLLDRTFRQGQQGQRGQQGQGQQGQGQMGQQGQQG
                     QQGGQPGQGQFGQQFGQRPGQGQPGQGQPGQGQNGQPVPGQGGDPGALAQDQQGLRDK
                     LDKIIQGLGKNGVQMPGGLDQAGREMGQSKDNLAGNQLQNAEGAQQRALEQLRDSAQQ
                     LAKNIMTANGQQPGDGKDQQNGERTDPLGRPLDQAGSMAGGNIKIPDQTDLARAREIL
                     EELRKRAGENERAPEELDYIERLLKQFP"
     gene            complement(14560..15828)
                     /gene="lysA"
                     /locus_tag="VGM72_10625"
     CDS             complement(14560..15828)
                     /gene="lysA"
                     /locus_tag="VGM72_10625"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEY4265763.1"
                     /translation="MNHFHYKNGILSAEDVPLTEIAEAVGTPFYCYSTATLTRHYNVF
                     AAALPKDSLIAFSVKANGNIAVLKTLGRLGSGADVVSGGELKKALAAGIPANRIVFSG
                     AGKTREELRLALETGIYQFNVEGEPELEALSQIAVSLGATAPITIRVNPDVDAKTHAK
                     ITTGTYETKFGIPWTRARETYARAAKLPGIEVVGVDVHIGSQITDLDPFRAAFDRVRA
                     LIAELRGDGHRILRADLGGGLGVPYRLDNAPPPDPDAYGAVIAAATRGLDVQAILEPG
                     RLIAANAGILVSRVIYAKPGESKTFLVLDAGMNDLIRPALYDAHHDILPVLAPDPKLQ
                     TRRYDVVGPVCETADTFARDRDLPETRGDDLMAIMTAGAYGAVMASAYNVRPPAAEVL
                     VNGREWCVVKPRMTLEALIALERVPGWLEH"
     gene            complement(15839..16057)
                     /locus_tag="VGM72_10630"
     CDS             complement(15839..16057)
                     /locus_tag="VGM72_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265764.1"
                     /translation="MSPRLLPFALIALALASCGVKANLERPLGAMMQTQTMQAQKQIL
                     QNPQKDPSLPPRPLGEPGGTTPPYTTGP"
     gene            complement(16054..>16743)
                     /locus_tag="VGM72_10635"
     CDS             complement(16054..>16743)
                     /locus_tag="VGM72_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEY4265765.1"
                     /translation="SARDFALEFLAAASIAGAHLSRLAEEIVLWASPAYGFIRLPDAF
                     STGSSIMPQKRNPDAAELSRAKVGRILGAFVGLLTVLKGLPLAYAKDLQEDKEPVFAA
                     SDALDLALAAMAGMTSALTVDKNAMRRAAEKGYPTATDLADWIVAKRNKPFREAHHIA
                     AKAVARAETLGVALDALPLAELKKLDPKITKDVYKVLSLEASVNARTSFGGTAPSRVR
                     EQIGFWKEQLQ"
BASE COUNT         3004 a         5273 c         5413 g         3053 t
ORIGIN      
        1 ccgccggggc cgacaatctc cgcgcagcgc cgggcgagcg ccttgggatc gcgcacatgt
       61 tccagaatgt tacagcagag aatggacctg ggcttgaaac tgcggatgcg cggcaggtcg
      121 gcgtcggaaa ggatgtcggc cttgatatcg atcccgtgac cttcgcgcgt gtccaggtga
      181 atgatttgca cgccgcgggc gcgcaacggc gcgaacagcc ggcgttcggc ccaaggctgc
      241 gcgacctcgc gaaaatcgct ggtgctgctg ccgacgttca aaagcgggga caaatcgtcc
      301 ggcgtccgct cccgcagcaa catctccagc caagccgcct cggtttcgaa catttccgcc
      361 tcgcttcggt cgggccggag actgcggcaa aggcgtgcgg ctcgcaacgc tttgattttg
      421 cccgggcgcg cccctatcaa tagggcctgc ggcaagtccc gcgtgtgtat tgcgcgttcg
      481 tgaggcgcgc ggattttaag gtcgatcgtt gatgcgaaat gagtccggca cgcttgaaga
      541 tctccgcggt gaaatcgatc gcatcgatga tgccttgcac gatcttctga tccgccgcgc
      601 cgatgtcagt ctggcaatcg cgaaagtgaa gcagccggtg cccggcgggg ccggcgcgtt
      661 catccccgcg ctcaggccgg cgcgcgaagc cgcgatcctg cgccgcctat tggcgcggca
      721 caaaggcgac cttccgcccc aggtgctggt tcgcatctgg cgcgagatca tcgcggccag
      781 cctgcgggtc cagagcaagt ttcaactgca catctacgcc tccgacaatc attcaggatt
      841 ggccgatctg gcccacgccc attttggctc gcagaccccg ctccgcacgc atacgcgttc
      901 ttccatggtg gtgcacgcct gtgcggagga gcctcattcg ctggggatcg tgccgctgcc
      961 cgagatcgag gaggcagggc ccgcatggtg ggcgcaactg gctcccgccg gcgagaaggg
     1021 gccgcgcgtg gtcgccaagc tgcctttcgt ccaggatggc gaggatggtc tttcggccta
     1081 tgccatcggc acggtggaac aggagccctc gggggacgac acgacgctgc tcctgctcga
     1141 gattgcaccg ggcgtttcac gcgcgacgct gcaatctttt ctcaagagcg ccgggctgaa
     1201 cgcaaagctg accgccgccg gccggctgcg ggaaaaaaat gcgccggacg aaatcctcct
     1261 cgaagtgaag gagttcgtcg gcaaggacga tccgcgtctc accgcgctcg ccgatgcggc
     1321 cggaggcgcc atcatccgcg cggcgccgat cggggggtac gccaacccgg tcatgctgcc
     1381 cgcggcggtg cgctgatggg tttcgcgcca tgactccatc ccccaaaccc ggcgtcatgg
     1441 acatcgatgc ctatgtgccc ggccgctcgg aggccaaggg cgcggcccgc gtttacaagc
     1501 tgtcctcgaa cgaatcgccg tttggcccca gtcccaaggc gatcgctgcc tatgaagcgg
     1561 ccgaacccgt gctcggcgtg tatcccgaag gcagcgcccg catcctgcgc gaggccatcg
     1621 ccgcgcatta cggattggcc gcggaccgga tcgtttgcgg caatgggtcg gacgagattc
     1681 tgacgctgct cgccaattgc tatgtgcgtc cgaaggacga ggtgctgttc ggcgcgcatg
     1741 cgttctcgct ctacaaaatc gtgacgctcg ccaatagcgg cgttccggtc gaagtgccga
     1801 cgcgggatct gcaactcgat gtcggcgatg tcatcgcccg cgtccgtgaa aagacgcgcc
     1861 tcgtctatat cgcgcatccg aacaatccga ccgcgaccta tattccggtc tccgagatgc
     1921 gccggctgca tcgcgcgctt cccgattccg cgctcctcgt gatcgacgcg gcttacgcgg
     1981 aatacgtaca gaaaaatgat tacgaggcgg ggatcgaact cgtcagcacg accaacaatg
     2041 tcgtgatgac gcggacgttt tcgaaggtct atggcctcgc gggaatccgc atcggctggg
     2101 cctattgccc caaacccgtg gccgatgtgc tcaatcgcgt gcgtgcgccc ttcaacatca
     2161 atattgcggc acagcgcgcg gccgtggcgg cgctggccga ccggggacac acggaaaacg
     2221 ccatacaaca caatgccttc tggcggagct ggctgaccga acagattcgt ggcttgggcc
     2281 ttcgcgccga tgacagcgcc gccaatttcc tcctcatcca tttccccgat acaccgggac
     2341 gcagcgccga cgatgcggat cgctttttga tggcgcgagg tataattatg cgggcgtgcg
     2401 gttcctacac cctgccgcaa tgtttgcgtc ttactgtcgg cagtgaggaa gcaaaccggg
     2461 cggcgatcgc cgctttggat gagttcatgg catcacaatg aacgacacca gtcctatctt
     2521 tgaaaagatc gcgctcatcg gcgttgggtt gatcggctcg tccatcgccc atgccgcgcg
     2581 ccgtggcgga ttggcgcgcc atatcgcggc ctatgtcccg cgcgccgaga ctcgcgcgcg
     2641 cgtggaaaaa atcggctttg cggattcgct gcatgaccag ttggagtctg ccgttgccgg
     2701 cgccgatctt gtcgtcttgg cgacgcctgt cggcgcctat gccgcgctgg ccgcgagcat
     2761 cgcgccctat cttaaacctg gcgcgatcct caccgatgtc ggctcggtca aaggccaggt
     2821 gataaaagag atcggaccgc acgttcccaa ggatgtgcat ttcattcccg gtcatcctgt
     2881 cgcgggcacc gagcaatcgg gcccggaagc aggcttcgcg gagctgttcg acggccgttg
     2941 gtgcattctg acgccacctg ccggaaccga tccggaagcg gtggagagat tgaaagcgtt
     3001 ctggcagcgt tgcggcagcg ccgtcgagac catggatgcc aagcatcatg atcttgtgct
     3061 cgccatcacc agccacgtgc cgcatctgat cgcctacaac atcgtgggca cggccgccga
     3121 tatggaaacg gtcatccaaa gcgaggtgat caaatattcc gccagcggct ttcgcgactt
     3181 cacccggatc gcagcctccg atcccacgat gtggcgcgac atcttcctta acaatcgcga
     3241 agccgtgctc gaaatgctgg gacgcttcaa cgaggatttg gcggcgcttc agcgtgcggt
     3301 gcgttggggc gatggtgacg cgctgttcaa tcttttcacc cgcacccgtg cgatccgccg
     3361 ttccgtgatc gaggcggggc aggatacgcc cgcacccgat ttcggccggt cagggaacgt
     3421 ggccggaggc cgctgagccc gaagaaaacc gcatgtcgcc attggcgaag gccaggccaa
     3481 aacttgttgc ggtcttgccc ttcgggccat cgccttggag cgccccggtc acgcggtcat
     3541 tctcgtcgag cgcgagatct cctttcagat cggcatgtcc gccgggccag tcgagcgcga
     3601 gattgttgac ggtcagcgtg cccgtgcggc cgcgccatgc cgccgccgca tcggcgagac
     3661 tcatcatgcc ggcccgcaac gcatcgagcg catcgccttg cgtgaatttt gcgcgcagat
     3721 cgatccgcgt cagttgagtc tcgtccatgc cgccggcata gccgtttccg attttcgcat
     3781 tgtcgatcat gagcgccaga tcgatgctgt cgtcggggcg cgccaagaga tgaaactggg
     3841 cgcgggccgc caccgcggta cggttgggct cggcgccgcg cgtcgcatct ttcacttgcg
     3901 gctccagaag gtcgaggtcg aaccgcgtaa gacgatcatt gaccaggatc gcgctggccc
     3961 gcgcaatgcc gggcgtgacg aatgtcacgc gcggcacggt tccgcgcgtc agcggcggct
     4021 gcgcaaagga ttgcagcccc gacgcttcga aaatatagcg gttctgaccg tacgaaagtc
     4081 tgtgcagcgc aagcttttcg gtgcgccagg cggtttcgcc ttccggcgcc tgatgcgaga
     4141 acgtgacgcc ggagagcacc gcatcgatgc ggaaagggta gccgccgacc gatttctccg
     4201 cgaaggcgaa gacgattccg gggatgacct cgccgccatt ggcgcggtcg agacgtgctg
     4261 ccagcgtatc ggcctgcacg cgccagaaca cgctgtacag gacgataacc agaagcagca
     4321 ggctgaccgg gccataaaag aagatgcgat tggaatagga catggatcac gaaatagggt
     4381 tgagcccggg cactgtgtca ggtcaggtgc atttggaata gccgcaggat atgcaggtgt
     4441 cgcagccttc gagcttgacg aatcctgcgg cgctgcatcg tggacaggtg cgcacggccg
     4501 ccccttcggc acccgcaacg gcagcggccg cgcgctgcgc gtcggattcg ccggagccag
     4561 gctgtgtcat gaagccgatg gcgatcagat gccgctcgat cacttcaccg attgcggcaa
     4621 ggagcgaggg gatataacgc ccgcccatcc attgaccgcc gcgcggatcg aataccgatt
     4681 tgagttcctc caccacgaag ctgacgtcgc cgccgcgccg gaacaccgcg ctgatcatgc
     4741 gggtgagggc aaccgtccaa gcgtaatgct ccatattctt ggaattgatg aagatctcga
     4801 agggacgttt gcgcccgccc tcctcgatat cgttgatggt gatgtagaag gcatggtcca
     4861 gatcgggcca gcgcaccttg taggtgtggc cgggaagatc gccggggcgg tcgagaggcc
     4921 ttgtcatgta aaccacatcg gcaggctcgg cgcgttccgc gaagcggtag gccggcgcgg
     4981 tcccgggcaa tgcccgcgcg ccgcgtgccg cgatcgatgg aaccgcttgg accgcaggcg
     5041 caagattcgc gggcagaacg gcggaaggcg cgagcccctc ggcgcgctcc gtttccagca
     5101 cggctccggt caccggattg gggcgatagg tggtgcaccc tttgcagccc tcccgatagg
     5161 cggtgcgata gacgtccttg aagtcggcga aggaaatctg cggcggacaa ttgatggttt
     5221 tggagatcga gctgtcgata tatttctgag ccgcggcctg tacggcgaca tgctctgacg
     5281 gactaagctc ctgggccgtg acgaaataat cgggaagctg cgtatcctcg ccgaagaggc
     5341 cgcggaacct gcgataggcg taatcgctga ccttttcttc gcgccgggtt ccgtccggca
     5401 tcaatacatt gcgcgtatag gaatgggcga agaccggttc gatgccgctg gagatgttgt
     5461 ccgcgaacag cgagatggtg ccggtcggcg cgatcgaggt caaaagcccg ttgcggatgc
     5521 cgtggccggc gatcgccgcg cggataggct cgggaagacc ggcgatgttg ggccgggcca
     5581 gataggcatc cttgtcgaac agcgcgaagg cgcccttttc cgcggccagt tcggcggaag
     5641 ccccataggc ctcatcccgt acgatcctga gccaatgttc gatcagcgca agactgcgcg
     5701 aggagccata gcgggcgcgc gcgaagatga gtgcatcggc caagcccgtg acgccgagac
     5761 cgatgcggcg cttctgcttg gcttcgtctt cctgcgcggg caggggaaag cgcgaaacgt
     5821 cgatgatgtt gtcgagaaag cgcaccgcca cccgcgcgag ccgcgccagc ttcgtttcgt
     5881 cgaggcgggc atcctccgta aacggatggt ccaccaattt ggcgagattg atggaaccga
     5941 gcaggcatgc gccatagggc ggcaggggct gttcgccgca cgggttggtc gcgtagatcg
     6001 tctcgcaata ggagagattg ttcagcgcgt tcacacggtc gatgaagatc acgcccggct
     6061 cggccgcctc ataggtcgta atcatgatct tgtcccacag cgcgcgcgcc gaaagcgtcc
     6121 gatagaccgt gccgtcgaac gtcaggtccc aggaatcgtc cgtctcgacc gcgcgcagga
     6181 atgcgtccgt caccagcacc gagaggttga aattgcgcag gcggtttccg gcgcgcttgg
     6241 cagcgatgaa atcctcgata tccgggtggg agcagagcag cgtccccatc atcgcgccgc
     6301 gccgggcgcc ggccgacatg atggtgcggc acatcgaatc ccacacatcc atgaaggaaa
     6361 ccggtcccga ggcatcggcg cccacgcctt tgacgggcgc gcccttcggc ctgagcgtcg
     6421 agaaatcata gccgatcccg ccgccctgct gcatggtgag ggcggcttcg cgcaaatggg
     6481 cgaaaatgcc cgagagatcg tcgggcacgc ggcccatgac gaagcagttg aaaagcgtca
     6541 cggagcggcc cgttcccgcg ccggccagga tgcgccccgc cggcaggaaa tgatgaccgg
     6601 caaggcttgc ctcgaacgcc gccgcccatt cgtgccgctt ctcgggcgct tcggcctcag
     6661 ccgcggcgcg tgccacgcgg gcccagcttt cctcgaaact gcggtcgagc ggcgtgccgt
     6721 cggccgcctt gaggcggtat ttcatgtccc agatctgatg ggagagtgcg tccacgtttc
     6781 ggactttctc aaaaacttag tggctcagag gcttggtggg tctttcacaa cgcatgatgc
     6841 gggcggattg gggtccggat catcgcatcc gtagcgcgag agtcaactgc atgttctctt
     6901 tttgttcgtt cgacgcagag gcccagcgtc aaccggcagg ataggtcgca tgcacccgcc
     6961 gcgaacgctc cagatatagg agccaggcaa cggcaagaac aatctgccgc agaccggcga
     7021 cgaccgcggg accgcgcaac tgattggcta gtgcgcgatc ggaaagttgc cataccgctc
     7081 ccaccagcgc gatgcctaat cccaggaccc cgagcgtcaa catggtgatg aaataaatct
     7141 tggcaatgga ggtggcgttc gggcgccgcc gatagagcgc gatgcccgaa tagaacgcga
     7201 agccggccag ccccagttcc agcacggctt cgtagacgag cgcgttctgc aacgtcgcgg
     7261 atcgcgccgc cgcctgccac gcgccgacga gacccagcac cgccagaagc gggatgagaa
     7321 gcgtcagata aaggcagagc gccagaagcc agccgcgcac accctgcagc cgggtctctt
     7381 gtgtgacaac agcatcgcgc gaactcatct cgtccccctc atgctcaaca ccttataatc
     7441 caggcgcggt ggatcgcgaa ttggcctcgc gcccagcctc ggccaacagg cggttcgtcg
     7501 cgtcctcggt gcggcgctga agttcgtcca tgaacgcctg gcgtgtaagg cccggtggaa
     7561 tgggttcgag aaattccacc acgatgcggc caggccgctt caggaagccg gtccagaaca
     7621 gtcccgaatt gtgcgccgcc ggcacgcagg caatgtccag caatgcgtaa agcgccgcta
     7681 ccccgggttt atagtccggc gcgtcgccgg gttttttgcg cgtgccttcg gggaagatga
     7741 cgatgggacg gccttcgttg ctggcacgcc tggcttcggc ggccatggcg cggatggcct
     7801 tcgcgccggc ggaccggtcg atggcgatca tccgcatctt gcggcaatac cagccataga
     7861 gcggaatcct cagcagcgat tgcttgatca cgatggcggg tcccggcaga aggcagagaa
     7921 acgcgatggt ctcccacatg ctgaaatgtt tcgaggccac cagcacgcgc ggctcggcaa
     7981 aggtgccgcg cacttccagt ccggtgccgg cgatccattt caatccgaac agcgagagct
     8041 tcgcccaaat gcgcgccgcc gccagggtcg cgcccggcgg cagcagaagc aacggcagga
     8101 gcccgacatt catgatgacc gaaatcgaga tgaaccaggc gagaaacagc gccgagcgaa
     8161 cgataaggat catgcgtcac gcctgccaag cgctccggcg acgcaaacac gtacgatgct
     8221 ggcgagatat ttgacatatt cgccattgag acgtttggcg ttttcccagg acagcatctt
     8281 cacggcttcg gccgataccg gataggggat cagcgtcacg ccgggcattt cggcgccgaa
     8341 ttccaccagg ctgcgcggca tgtgataagt ggccgtcacg acaatgaggc tgccatagcg
     8401 gtgagcgcgc acccagcgcg ccgcctccgc ggcattgccc cgcgtgtcga gagcggcaaa
     8461 acccaaatcg gcgcagcaat cgaaggcctg tccgccgtga agcagcgtct tcagagtggg
     8521 cttggagatg agcttgttca ccccggtgat caaaagccgc tgcccgccgc cggcctccag
     8581 cagtgtgacg gcgggaccca gacggccccc ttcgccggtg agcgccacga tggcgtcggc
     8641 cttggcgatg gcgcccgcgc gcggaagggg aagatcgacg acgaaaaacg aaaatccgat
     8701 cgcataaagc accaaggcca ggacggccaa ggaaagcgct gtacggaggg tccgtttgcg
     8761 ctttgtcctg ccgctcgcat gggtctgacc gtgctctcgc tgtttcatga taggatttta
     8821 gcgcagctaa tagagttcgt gcagcgcggc aacgaccgaa agccgggccg tcgcccaggc
     8881 gatggccgcc gcccctgcgg ggatgagcgc aagccagccg agatcgcgca ggggtagtcc
     8941 cagtggcggc aggaacgaga cgctcgaaac cccgagctgg tcgagacttc ccgcagcggc
     9001 gaagatcaat gtggcaatcg ccgcgccgca tgccgcggcg atcaacgcgg ctacgaaata
     9061 atgccattcg aaggcgcgtg cgatgaagcg gtcctgggct cccatcaggt gcagcaactg
     9121 cacgatctcg tgatgggccg cgagccccgc gcgcgtggcg aaggcaacgg tggcggcggt
     9181 tccgacagca atcagggcca ggatcgcaag tgccgcgaac acgacgctgt tggccgtgtt
     9241 cttgagccgc ccgatccagc gtccgtgatc gtccagcacc gcgtgaggcg ccgccgcctt
     9301 caaacgcttt tccagcgccg tcagatcggg tgtcgcgccg gccgcgagct gcacatcgat
     9361 catccgtgga aatggaagga ccgcaaccac cgcatcgcgt ccaagccagg gttccaccaa
     9421 agcgagattg tcggcatcgg agagcgcggt ggcatggagg attccgggcg tgccgctcag
     9481 aatccgcacc gcggcgttca cttcgcgggc cggtgcggaa tcgccttccg gcagaatctg
     9541 caccgtgatg cgtccggcga gcccagcctg ccaggattcg gcggtgcgct gcgcgatcaa
     9601 gacaccgccc agtgcaaggc cggcaaggaa ggccatcacc gcgatcacca gatcaaggga
     9661 tatggcgccg tcttcgcgcg gcaggatgcg gccggcgcgt ctttggcgcg gctcacgtgt
     9721 ggcgctctcg ctcatcggtt gctccgcatt tcgatgaccc gtccgtcgat cagccgcatc
     9781 tcgcgtgcgc gcgtgctttc caccagcgcc cggtcatggg ttgcgatcat caccgtggtg
     9841 ccgaaacgat tgagctcggc gaacagccgg atcagccttt cgcccatctc ggggtcgaca
     9901 ttgccggtgg gttcgtcggc gagcagaaga tcgggtttgg ccacgacggc gcgggcgatg
     9961 gcgacgcgct gctgttcacc gcccgacaat gtcggcggtc ttgcctgcag cctcgtgccc
    10021 aaaccgaccc aggagagcag ctcttcgacg tcgcggcgat attccgccgg gcggtggccg
    10081 gcgagccgca agggcagcgc cacattgtcg aacgccgaga gatggtcgag cagacggaat
    10141 tcctggaaca ccacgccgac gcgccggcgc aaggcgggca gatcggcgcg cttcagacgc
    10201 gccagatcgc gcccgaagag cgagatcaaa ccgcgcgacg gtttttccgc catgaagacg
    10261 agtttaagca ggctgctctt tcccgcgccc gatggtccgg tgaggaaggt gagcgagccc
    10321 gggtccagcg cgaaactgac atcgcgcaga acctccggtc cggcaccgta acgcatgccg
    10381 acattttcga accggaccat gctcctctcg acggggttgc gcgtagtttc gcccgaatcg
    10441 gcgttggctg accatagcga gcgtgcggcg gccgcgccac gatgctgtaa gctcccgcgt
    10501 gtgcgcaata gatgcgcgtt ctcccttgtc atgccgcgaa cgatgctgca atttccggtg
    10561 aagggcgcgc gattttgaaa ccatgatcct tacctgccca tcgtgccgga cgcggtatca
    10621 aaccgatggc gcacagttca agccgccggg ccgcaatgtg cgctgcgcca aatgcggaca
    10681 ggtctggtat caagccaagc ccgaacccga gaccgaggtc gagcccgaac ctgttctggc
    10741 gccgccgagc gtgccccctc ccagcaagcc catggcggcg gaacttgcgg cgcccatgca
    10801 tggcgcgctc gatttcggtg tggcatccgg catcgagccg gagccggcca gcgccgatat
    10861 tcgcttgcgt tcccgcggca cggcctttgc gcaggccgtg gggtggcttg cgctgatcgg
    10921 cctgatcggc gcgatcggct ggagcaccat tcaatatcgc gagaccatcg ccggcctgtg
    10981 gccgcaatcc gcatcgcttt acagcgtttt gggtttgccg gttaatgtgc gcggcatcac
    11041 gctgaccaac atcgcctaca agcaggaata tgaagacggc cagcaggtgc tctcggtcac
    11101 cggcaagatc gtcaacgtca gcaagcgcga tcaggtggtg ccggagatcc gcgtggtgct
    11161 gttggataat gccaagcacg aactctatca ttggacgttc gatgcgggca tccaaaccct
    11221 gaagcccggt acagagaccc ccttcatgac cagactttcg agcccgccgg ccgaagcccg
    11281 cgacctcaat gtgcgtttcg tggatagcgg cgagacgcgg tgatggctgc catgacattt
    11341 cgatccgccg catgatgggc gcggaggcgc aagagcgcat tctcttttcc gaagagcaga
    11401 tcgcaagccg cgtgaaagag gtcgcgcgcg agattgcggg cgcccccttg cgccccggca
    11461 ttgcggtgcc gattcttgcg ggcgccttcg tcttcgcagc cgacctgctg cgggcgctgg
    11521 cgtccgaagg attggacctg gagaccgaat tcatctggct cagatcctat ggagcttccg
    11581 acactccgtc cgaaatcgcg gtgctgaaag gccccggcga tttggttcgt gggcggacgg
    11641 tcctcatgat cgacggtgtg ctggaccgcg gagtcacctg cgcgcgcgcg cgccagcttc
    11701 tgatggcggc cggcgccgtc caagtgatct gcgcggtcgc cgtggtgaaa tcctatcccg
    11761 atgcgcggta ccgcgcagat tatgcgctgt tccgggcggg cgaggaattc ctctatggct
    11821 atggcatgga tcgcgcgagc ctcagccggg gcttgccgga catccgggtg acggaaaaca
    11881 aaaagctctc acgcccagcg taagagcttt ttgacttagg ggaactgctt cagcaggcgt
    11941 tcgatgtaat cgagttcttc cggggcgcgt tcgttttcgc ctgcccgctt gcgcagttct
    12001 tccaggattt cgcgggcccg cgcgagatcg gtttgatcgg gaatcttgat gttgccgccg
    12061 gccatggagc ccgcctgatc cagcggccgg ccgagggggt cggtccgttc gccgttctgc
    12121 tgatccttgc cgtcgccggg ctgctgaccg ttcgccgtca tgatgttctt ggcgagctgc
    12181 tgagcgctgt cgcgcagctg ttcgagggcg cgctgttgtg cgccttcggc gttctgcaat
    12241 tgattgccgg cgagattgtc tttcgattgg cccatttcgc ggcccgcctg atcgagccct
    12301 ccgggcatct gtacgccgtt cttgccgagc ccctggatga tcttgtcgag cttatcgcgc
    12361 aggccctgct ggtcctgggc aagggcgccg ggatcgccac cctgtcctgg gacgggctgg
    12421 ccgttctgac cctggccggg ttgtccctga ccaggctggc cctgaccggg acgctggccg
    12481 aattgctgac cgaactggcc ttggccgggt tgaccgccct gctgtccctg ttggccttgc
    12541 tggcccattt gcccctggcc ctgctgaccc tggccctgct ggccgcgttg tccctgctga
    12601 ccttggcgga aggtgcggtc cagaagctgc cgctgaccgc ccatgatgtc gccaaggccc
    12661 tgcagcgcct gattcatggc gttttcctgc ggcgtgcctt gaccctgctg gccttgctgg
    12721 cccggttgac cgggctgtcc gccgcggccg gccatcatct tcatgttctc catcatctgc
    12781 atcagggccg cgagcatctg gcgggcctga tcgcgatttc ccgtgcgggc gagatcttcg
    12841 atcgccttca ggaggtcctg gagatctttt tgcgacatcg cgcgcgcgcc gggcggcatc
    12901 tggcccatct gctggttgcc gcgttgggcg ttgcgcgcga gctgttgcat gtagcgcgcc
    12961 atagcctggc gcagcttgtc catcagggca cggatttctt catccggggc gccgcgttcc
    13021 aacgcgtcct ggagctgttc ctgtaccttg cgcaattctt cggcggcgtt ggcgagatcg
    13081 ccgtcttcga tggcgagcgc catgtcccac atgaaattct gtgaggtttt gaagcccgga
    13141 tcgttcttag ggctggaaag acgatagttc agcgaacgca gcgccagata attgctgaaa
    13201 tcatccttgt agaattgctg cggcccgagg ctcaacgcat ccagcgccgt ctgcaccttg
    13261 gggcgcgact tctcgccgtc gaccgcgagg gttttgcgct gctcgatcag cgattgtgcg
    13321 agcggctcgg tgaagacgcg ctgcggcagc ttgatcgtca acggcgcgct cgtgcccgtc
    13381 tgaccggcgg cgtccgtcgc ctgcagcgag atatgaacgt tcgaacccgc aaacgcatgc
    13441 gcggtgagat cgcgatacac cgtgtcggag accacgcgcc ccgtgccggg caccggcaat
    13501 tcgacggtga gcgtttcggc ggatgcgatc tcccggccct tgtcgtcgag cgggcggatg
    13561 cgggccgccg ccttcaccac gccgtaatcg tcctggcctt tgtaggaaag cttcagggcc
    13621 gcgttctgct gtgcggccgg cgtttccgtg aacgcgatcg tcggcggttt gtccggaatt
    13681 gtgatgaagt aataattccc atagctgtga gagcccagcc ggatcgacac atgggagttg
    13741 accgcgatgt tgagcttggc ttcgtagccg gcatcgcctt tgacgaattt gggctggccg
    13801 cccttgggga tcggccgcgc atcgatggtc gggcggctgg atgtgccgcg caaacgcatg
    13861 accaacgtgc tgttgacggg agcctggatg tcgcccgagg ctgtcatcac cgtggtgtcg
    13921 gtgagcgagc gcggcggcag gccggtgtaa atgggtggcg aaacccaggc gacgaagacc
    13981 gaattgtcga ccgcatcccc aaaaacaggg aacaggcctg agaccagcct ttggcccgat
    14041 tgcggacccg cgatcaggaa tccggccagc acaccgagca gcaccgcgaa gcgcaagcca
    14101 cgggggtcgc gtctgcccat gcccggcgaa ggcagcgcaa ggcgcagacc cttggccgaa
    14161 gcgagcagcc tgatgatgtg ggcccgccag agtctctcgg ccaagggatc gcccttgccg
    14221 gcggcgacga cgtccattcc ttccgtgatc ggacgattgg gaagatcgct gtcgcgttcc
    14281 acgcgccgcg cgccgtcttc ccagcggggg ttgcgaaagc gcgcgaagga gcgccagaac
    14341 acgaacagca gcgcgccgaa gaacgccagc agcagcaccg cgtgcaccgc acccggaata
    14401 aaggcaaacg cgccggtcag cgccagaatc atcccggcgc cgaaaatgcc aaggcctggc
    14461 cacatcgccg gccagattcg ttcccagagc agaatcaatc tggcggtcgc gacgaaacgc
    14521 tcggcctttt ggcggcgcgc ttcccgttcg ttcttgttct cagtgctcaa gccagcccgg
    14581 aacccgttcc agcgcgataa gcgcttctag cgtcatacgc ggcttaacca cacaccattc
    14641 tcgcccgttc accagaactt cggcggcggg cgggcgaaca ttataggcgg atgccattac
    14701 cgcgccatag gctcccgccg tcatgatcgc catcagatca tccccccggg tttccggcaa
    14761 gtctcgatcc cgcgcgaagg tgtctgcggt ttcgcacacc ggcccgacga cgtcataacg
    14821 ccgggtctga agtttcgggt ccggtgccaa aacgggaagg atgtcgtgat gggcgtcata
    14881 gagcgcaggg cggatgagat cgttcatccc cgcgtcgagc accaggaatg tcttggattc
    14941 gccgggtttc gcgtagatca cacgcgaaac caggattcct gcgttggcgg cgatcagccg
    15001 gcccggctca agaatcgcct gcacgtccag cccgcgcgtg gccgccgcga tcaccgcgcc
    15061 ataagcgtcc ggatcgggag gcggcgcatt gtccagccgg tagggtacgc ccaagcctcc
    15121 gcccagatcg gcgcgcaaaa ttctgtgacc atcgccgcgc agctccgcga tgagcgcgcg
    15181 cacgcgatcg aaagccgcgc ggaaagggtc gagatcggtg atctgactgc cgatatgaac
    15241 atcgacgccg acgacctcga tgccgggaag tttcgcggcc ctggcatagg tttcgcgggc
    15301 ccgcgtccag ggaatgccga atttcgtctc ataggtgccg gtggtgatct tggcgtgcgt
    15361 cttggcgtcc acgtcgggat tgacgcgaat ggtgatcggc gcggtggccc ccaggctaac
    15421 agcgatctgg ctgagcgctt cgagttccgg ctcgccctcg acattgaatt ggtagatgcc
    15481 tgtttccaac gcaaggcgca gctcttcgcg cgtcttgccg gcgccggaga agacgatgcg
    15541 gtttgccgga atgccggccg caagcgcttt tttcaattcg ccgccggaga ccacgtcggc
    15601 gcccgaaccc agccgcccca aagtcttcag cacggcgata ttgccgttcg ccttcaccga
    15661 aaatgcgatc agcgaatcct tggggagcgc cgcggcgaag acattgtaat ggcgcgtcag
    15721 cgtggccgtc gaatagcaat agaacggcgt gccgacggct tcggcgattt cggtcaaagg
    15781 aacatcttcg gccgacagaa tcccgttttt gtagtggaaa tgattcacgc aggtctgctc
    15841 acggccccgt ggtgtagggc ggcgtcgtgc cgcccggctc gcccagcggc ctcggcggca
    15901 gtgacgggtc tttctgcgga ttctgcaaaa tctgcttttg cgcctgcatc gtctgggtct
    15961 gcatcatcgc gcccaggggc cgttccaaat tggctttcac gccgcaggac gcgagcgcca
    16021 aagcgatgag cgcgaacggc agaagacgcg gactcattgc aactgctcct tccagaagcc
    16081 gatctgttcg cgcacgcgag acggtgcggt gccgccaaag cttgtgcgcg cattcaccga
    16141 tgcctcgagc gacagcactt tatagacgtc cttggtaatc ttggggtcga gttttttcag
    16201 ttcggccagt ggaagggcat cgagcgccac accgagcgtc tcggcccgtg ccacggcctt
    16261 tgcggcgata tggtgcgcct cgcggaacgg cttgttgcgt ttggccacaa tccaatcggc
    16321 cagatcggtc gcggtcggat agcctttttc cgcggcgcgc cgcatggcgt tcttgtcgac
    16381 ggtgagcgcg ctcgtcattc cggccatggc ggcaagcgcc agatcgagcg cgtcggaggc
    16441 cgcgaacacc ggctccttgt cttcctgcag atccttggcg taggcgagcg gtaggccctt
    16501 caacaccgtg aggaggccga cgaatgcgcc caggatgcgc cccactttcg cgcgcgacag
    16561 ttcggcggca tcgggattgc gcttctgcgg catgatggag gagccggtgg aaaacgcatc
    16621 cggcagccgg atgaatccgt aagcgggcga ggcccaaagc acgatttctt cggccaggcg
    16681 cgaaagatgc gctccggcta tggaggcggc cgcgaggaat tcgagcgcga aatcgcgcgc
    16741 cga
//
LOCUS       DASYEG010000246        15602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4146830, whole genome shotgun sequence.
ACCESSION   DASYEG010000246 DASYEG010000000
VERSION     DASYEG010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 15602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15602
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4146830"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VGM72_10640"
     CDS             <1..301
                     /locus_tag="VGM72_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265766.1"
                     /translation="PLGSQAGTLSAGFRFLPKGHAGENVMRNMMMVGVILVVLGIVGL
                     IVQNVHFTETKKVVDIGPLKVNSEEQHNVPIPTIAGIVAVLAGLGIVFAARRGTP"
     gene            506..1726
                     /locus_tag="VGM72_10645"
     CDS             506..1726
                     /locus_tag="VGM72_10645"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY4265767.1"
                     /translation="MAEPENASTSPLPLQGLRVLDVSQVMAGPFCSMLLGDMGADVIK
                     IEPPDGGDQTRRAMGFKLKGNDSLGFFNLNRNKRSLALNLKSKAARDVFYRLTETADI
                     LIENYRPGVTKRLGIDYETLAKINPRLIYASISGFGQTGPWSQRPGFDLIAQAMAGVM
                     SITGQPGGPPTKAGVPVSDIGCALFALYGIMTAYIGREKTARGQYVDASLFEAAIAFS
                     IWDISEYWGTGNVPTPLGTANRFSAPYQAVRAKDTSFVMGANSNRLWQTLCEVLERPD
                     LAADPRFGAIADRLLNRVALIAELEKSFAARNAADWIDILLAAGIPAGPILNYAEALA
                     SDHAKARQAVMEFEHPIEGRVKSIGFPVKLSETKQRVRLPPPLLGEHNDAILAELGID
                     EKTRAELRAEGAAP"
     gene            1723..2496
                     /locus_tag="VGM72_10650"
     CDS             1723..2496
                     /locus_tag="VGM72_10650"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY4265768.1"
                     /translation="MSEGRVHYTKADGIASVVFDRPEARNAMTWAMYGELATACEAIA
                     SDTDVRVAVFRGAGGAFVAGTDIQQFTAFTGAEDGIAYERRIDAAIDGIDRLEKPIIA
                     MVEGFCVGGGLAIAMACDFRIASPSARFGMPIARTLGNCLSVGNVARLLAQFGPGRTK
                     RMLMLAEMISAEDAHACGFVDVLAAAADIDVARAKMCKTLMGHAPITMRAAKEAIRRI
                     RTENLPDGSDLIRAAYGSADFKEGVEAFLAKRAPHWQGR"
     gene            2523..3059
                     /locus_tag="VGM72_10655"
     CDS             2523..3059
                     /locus_tag="VGM72_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265769.1"
                     /translation="MTDRILRSAAMSMALCFALLPPATARQWNPDTRGAAAEYTQIIH
                     AKPNGEFVFLWWAVPEAFPNDANTQVLKDVLSRYVFVGIAHGRNNGTGLSFDNIPDLR
                     IADAMGRQMSPIPANSAPEVTQTLNALQALQSPMRQGMHWFVFEGSSTHSCTPGKLSV
                     PFAGETYTFDTPIPGCQK"
     gene            complement(3095..5407)
                     /locus_tag="VGM72_10660"
     CDS             complement(3095..5407)
                     /locus_tag="VGM72_10660"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY4265770.1"
                     /translation="MNALLPEALPRRDFLKGAGALMIGFSLAGALYPGAASALEIGPY
                     GPPENQIDSWIAIGEDGHATLFTGCCELGTGSSTGLMQIMAEELDLPFDRVRLFGPDT
                     DRTPDQFVSSGSRTISAHSRPIRQAAAEARAALVDLAAQRLAVPAEQLVTADGVVSAR
                     GNAAKHISYAELVGGREFNLTVTGKVKPKAPKDYKLVGQSQPRWDIPGKVFATFTYMQ
                     DVKVDGMLHGRVLRPPSHGASVMSIDENSVAHIPGLVKVVRVGDLVGIVCRREEQAIA
                     AAQALKITWSDWAGLPDMKDLHATLRNLPEFSAGYPKQSPGGVLAKSGDVAAGLGKAA
                     KTVKATYLSPYHHHGSIGPSCAVADVRADGVTLWCGTQTPYGMREAAAKFLGLPNEKV
                     HLIYTEASGCYGQNGADDVSIDAIVLSRAVGKPVRVQWSRADENGWEAYKAARISECS
                     GGLDADGKIIAWDARTFGLSGYSRPEYHEPKHGGEPGSLVTAQLAGWTKPGLEEGFSG
                     AAGNFEPVYEDLPNKRVVFTYLGPASHRQGPLRIRVGSMRGVGSPDNIFVAECFMDEL
                     AAAAGEDAIAFRLKHSPRPRGVAVLKAAAEKAKWETRPSHSKPSDGDIALGRGVAMLG
                     SNTDTNVAGIFEVAVNRKTGMVHVQRVTIAQDCGLVVNPNAVRNQIEGGVIQSISRAL
                     KEEVTFDRSRVTSLDWASYPILTFNEIPDAIDIVLVDRSDLPPMRVGEPASESVWPGV
                     ANAVYDAIGVRLRQLPLTPQRVLAGLAGGA"
     gene            complement(5419..5886)
                     /locus_tag="VGM72_10665"
     CDS             complement(5419..5886)
                     /locus_tag="VGM72_10665"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY4265771.1"
                     /translation="MATTTTLRVNGKSVTATVDDPSTPLLYVLRNDAGLHGPRFGCGL
                     GQCGSCTVHVNGAPVRSCMRPLSSIGARDEIVTLEGLGTSEKPHKLQAAFVEEQAVQC
                     GYCINGMIMQAAALLKKNPKPSVPQIKAALQNNLCRCGTHMRIVRAVRRASEA"
     gene            complement(5955..7301)
                     /locus_tag="VGM72_10670"
     CDS             complement(5955..7301)
                     /locus_tag="VGM72_10670"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4265772.1"
                     /translation="MQVDVDRLIDERPISALQIRVFILCAAVTVLDAIDSQSIGVAGP
                     LIAQGLKIAPADFWPAYSAGLFGAAIGAFAFGPISDRFGRKPTLVFTTLLFGIFTCLT
                     VFAGSFPELVAFRLAAGLGLGGATPCFITMAAEYAPHRSRAMLVSLLWAGYPLGNAIG
                     GFMTSYVVKHFHWSMVFYVGGIPTLFVALLLLLFMPESLRFLAARGSGERATHLARRL
                     DPDLPEAMTIAARTFAAPKAPLRDLFANGRAAGTILLGLMLYFGFWTTTVIVLQTPTI
                     FREAGMALSASAFLVAVYSLVATCGMAIAGWLLNRFGPVLGIALPFAGGAVVLAALGL
                     VADTPIAAGAIMMMLGLTVSVASSGVIALAAISYPTAMRSAGTGWVLAMGRFGQVCSP
                     YAVSKMLGLGFSPGPILAVMALAPLLGGLCVLLKSALAGGPSPARGDAVVEAEKTA"
     gene            complement(7372..8256)
                     /locus_tag="VGM72_10675"
     CDS             complement(7372..8256)
                     /locus_tag="VGM72_10675"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY4265773.1"
                     /translation="MPLRFDDIGNRLKAFRLGSGLSAEEVAKRIGISRTALYRFEKGE
                     VVKIETLEKLAELLEVSLPTLLGVGVEYIASAVTYFERMRQIEETAEHIIVLAGPLSY
                     LLASDAFNAVLGQLLKESISDIGADRARGEAEVEEIMAVLKQRKETYRRRQPAIVNLI
                     AASEIARFLINGMVGRFDLPEKLRRERRMLASSEVEYFAKLMEDEPIGVQIGIVPETL
                     PHTGFQIFRQPDRQLLSLSPFRLGEEPNVRVGVAMITSAQEALALHHRAAERMWQRAL
                     KGAAAAEYLRALIARHRE"
     gene            complement(8272..8766)
                     /locus_tag="VGM72_10680"
     CDS             complement(8272..8766)
                     /locus_tag="VGM72_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY4265774.1"
                     /translation="MSEKRRIAVTINGKTYDEEVEVRFTLADFMRQQLGLTGTHLGCE
                     HGVCGACTILLDGLSARSCLMLAVQADGHEILTVEGIAPSADKMHPLQEAFRDNHGLQ
                     CGFCTPGMLTTLIEFLRDNPDPTEHEVRIAISGNLCRCTGYQGIVAAALDAAQRLKAI
                     KDTP"
     gene            complement(8763..9629)
                     /locus_tag="VGM72_10685"
     CDS             complement(8763..9629)
                     /locus_tag="VGM72_10685"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY4265775.1"
                     /translation="MKPPPFRYYDPGTVEEAVGLLSRLENAKLLAGGQSLMPMLNFRY
                     VLPDHLIDLNKIAALAYLREEGGALELGGMTRQRDIEFSDVVKTRFPILHEAILNVGH
                     RQTRNRGTIGGSLCHLDPSAELVTMAAAHDAVVTAAGPNGTREIPFAEFPVAFMTPAL
                     EADEVLTKVRFPRWPAGHGYGFVEFARRHGDFAIASAAALLELGANGRIARVSLTLGG
                     VAVAPVRMREVEKALVGETPSEELFRRLCEECRKVNAIEDVHAPASYRQHLATVMSRR
                     ALVAAMERVGRA"
     gene            complement(9642..11069)
                     /locus_tag="VGM72_10690"
     CDS             complement(9642..11069)
                     /locus_tag="VGM72_10690"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY4265776.1"
                     /translation="MTTQDLRSWIARMEAAGEIQHISGAEREEEIGGIVDFYQRKTGN
                     PAVLFDDVPGYPRGHRVIANILTSIRRIHLTLGNPADSSAIDLVHYWRRYMKEAKSIP
                     PQTVKTGALFENVAEGKDIDIFKIPVPRWHELDGGYYIGTGDMVVMKHRDSGWVNYGA
                     YRVQAHEPGVASIMCSKGKHGDLIMRSYHEKGEPCPIAVVCGMHPLLFMIAGLEIPYG
                     KNEYDAAGGLLGEPVEVIAGPRTGLPIPAHAEIAFEGFVYPDDLIKEGPLGEWTGYYA
                     GGLKKEPAIRIETFMHRNNPILMGAIPGVPPDDDSFYRGTYRSGAVWNQLEAAGIPEV
                     KGVWAHEAGGSRLWLTVAIRQLYAGHSKQAGLIASQCHAGAYANRFVVVVDDDIDPAD
                     MDKVLWAMCTRFDPREDLEVIKGCWSTVLDPMVYSESDRRNARVVIDACRPWARRDTF
                     PAVVRNSKELDERIRAKFAKLLPRS"
     gene            complement(11094..12158)
                     /locus_tag="VGM72_10695"
     CDS             complement(11094..12158)
                     /locus_tag="VGM72_10695"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003645.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEY4265777.1"
                     /translation="MDRVALSALYTLPPETAHHAVLFALEHGLVGARREPDDPILKTR
                     VMGLEFSNPIGLAAGFDKDARVVDAMLGLGFGFVEAGTVTPQPQRGNPKPRLFRLDED
                     AAVINRLGFNSGGVEGFARRLAARYHRRRNPGIVGANVGKNRDTADGAADYVAAIETI
                     AGLSDYLVVNISSPNTPGLRALQARAPIEDLLKRVLGARARNAPTGKKPPLLAKVGPD
                     LSPEQLADIAEVALATGVDGLIVGNTTINRPESLKNPQRFEAGGLSGAPLLQPSTECL
                     SSLYRLTKGRIPLIGCGGVASGADAYAKIKAGASLVQLYSALVFEGPKLANRIKRELA
                     DCLKRDAYLSVADAVGRDHV"
     gene            complement(12179..12775)
                     /locus_tag="VGM72_10700"
     CDS             complement(12179..12775)
                     /locus_tag="VGM72_10700"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY4265778.1"
                     /translation="MEINNAFEVPLPPADAWKVLMDIPRIAPCMPGAELTGVVDKDTY
                     SGKVSVKLGPVALTFAGQVKFTDIDDVAHAARVKAQGKDSKGRGGANANVDFHLEPSA
                     LGTRVLVKTDLTLSGAVAQYGRASGMIQDVAQQLIGQFANSLKAQLDASREPARPEIA
                     QSGKQVEPPKPKAISGFALLAMVLKSFFRRLFGGATKS"
     gene            complement(12787..14067)
                     /locus_tag="VGM72_10705"
     CDS             complement(12787..14067)
                     /locus_tag="VGM72_10705"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY4265779.1"
                     /translation="MAAGRLNTLSACEIARGVAAGAFTAEAVTRDCLARIEERDGAVK
                     AWAFVDPELALNQARAIDRGAVKGVLAGVPLGIKDIFDTFDMPTDMGSSIYHGNRTFA
                     DASSVALARRAGAVILGKTVTCEFAGLTPGVTRNPHDLDRTPGGSSSGSGAAVADMMC
                     AVGYGTQTGGSVLRPASFCGVVGYKPTYNLIARGGIKFAAESRDTIGLLARSVDDADL
                     IASVLIARPLSPRRDTPPRIGLCRTHLWEHALPESKRAVEDCAQRFETAGAIVRDAVL
                     PENFNALAEARTIVNPVERARSMAHEWNTNRALISPGLTRQIEEGLSIPHARYVASLK
                     RMKECRDMLPALFGDFDVLLVPCVTGEAPRGLASTGDTRFQEFWTALHTPAITLPTHK
                     GPHGLPIGIQLVGQLYADEILLSAARWAAERLGI"
     gene            complement(14067..>15602)
                     /gene="tcuA"
                     /locus_tag="VGM72_10710"
     CDS             complement(14067..>15602)
                     /gene="tcuA"
                     /locus_tag="VGM72_10710"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEY4265780.1"
                     /translation="RAAGRKHMGERPAPGLEKMNEWDVIIVGGGNAAFCAALAAREAG
                     ASVLMLEAAPEDESGGNSRFTAGAMRVVYNDVEDITALVPDLSQAEIDTSDFGTYTQD
                     QFFDDMARVTQFRTDPNLCEALVTKSLPTLIWMREKGIRFVPIYGRQAFKIDGKFKFW
                     GGLTLETIGGGPGLVDMLTASGKKNGIDIRYSTRATDLLYDGVRVSGVRYKHGNEVGE
                     LKARAVVLASGGFEANAEMRTRYLGPGWDLAKVRGTRFNNGEGIRMALAIGASPYGNW
                     SGCHAVQWEMNAPEFGDLAVGDQFQKHSYPFALLVNATGKRFVDEGADFRNYTYAKYG
                     RVVLEQPGQFAWQIWDKKTKHLQRDEYNIRQCTKVTANTIEELAKKLDGVNAEQFVKT
                     IAEYNAAVNTDVSFNPNIKDGRGTQGLAVPKSNWANTIDTPPFEGYAVTCGVTFTFGG
                     LRIAEDGQVMNLDYQPIPGLYAAGELVGGIFYFNYPGGTGLTSGSVFGKIAGSTAGKA
                     ARG"
BASE COUNT         3017 a         4993 c         4781 g         2811 t
ORIGIN      
        1 gccgcttgga tcccaagccg gaaccttaag cgcaggcttc cgtttccttc ccaagggcca
       61 cgctggggag aacgtcatgc gcaatatgat gatggtggga gtgatcttgg tggtgctggg
      121 catcgtcggc ctgatcgtcc agaatgtcca tttcacggaa accaagaaag tcgtcgatat
      181 cggcccgctc aaggtcaatt ccgaggagca gcacaatgtg ccgattccga cgattgctgg
      241 aatcgtcgcg gttctcgcgg gtctcggcat cgtttttgcc gcccgccgcg gcacgcccta
      301 gacgaccaac gcggcagaac cccaaatccg gcgcgcagat aatcggcgat gccgccgatt
      361 gacgattcag aggcgaggct ttcaacaagg cgccgcccca aaagaatcgc ttcactttcg
      421 cgtcccgcgg atgcgaattt gcgctcagag tgtccgttgc ccgcaggctt tcccagcgcg
      481 cctcgcggga ctttgaggag cagccatggc cgaacccgag aacgcgtcca cgtccccctt
      541 gccgcttcag ggtttgcggg tgctggacgt gagccaggtc atggcggggc cgttctgctc
      601 gatgctgctc ggcgacatgg gcgcggatgt catcaagatc gagcctcccg acggcggcga
      661 tcagacacgc cgcgccatgg ggttcaaact caagggcaat gacagcctcg gcttcttcaa
      721 cctgaaccgc aacaagcgca gtcttgcgct gaacctgaag agcaaggcgg cgcgggacgt
      781 gttctaccgg ctgaccgaaa ccgccgacat cctgatcgag aattatcggc ccggtgtcac
      841 caaaagactc ggcatcgatt acgagacgct cgccaagatc aatccgcgtt tgatctatgc
      901 gagcatttcg ggcttcggcc agaccggacc gtggtcccag cgtcccggct tcgacctgat
      961 cgcgcaggct atggcgggcg tcatgagcat caccgggcag ccgggcggac cgccgacaaa
     1021 ggccggcgtg ccggtgtccg atatcggctg cgccctgttc gcgctctatg gaatcatgac
     1081 cgcctatatc ggccgcgaga aaaccgcgcg cggccaatat gtcgatgcct cgctgttcga
     1141 ggccgccatc gccttcagca tctgggacat ttccgaatat tggggcacgg gaaacgtccc
     1201 cacgccgctc ggcaccgcca accgtttcag cgcgccctat caggcggtgc gcgccaagga
     1261 cacatccttc gtcatgggcg cgaacagcaa caggctctgg cagaccctgt gcgaagtgtt
     1321 ggagcggccc gatcttgccg ccgatccgcg atttggcgcg atcgccgacc ggctgctgaa
     1381 ccgcgtggcg ctgatcgcgg aattggaaaa atccttcgcc gcccgcaatg ccgccgattg
     1441 gatcgatatc ctgctcgcag ccggcattcc ggcaggcccg attctgaatt atgccgaggc
     1501 gctggcgagt gatcatgcca aagcgcggca ggcggtgatg gaattcgaac atccgatcga
     1561 aggccgcgtg aaatccatcg gctttccggt gaagctgtcg gagaccaagc agcgcgtgcg
     1621 gctgccgccg ccacttctcg gcgagcacaa tgacgccatc ctggcggaac tgggaatcga
     1681 cgagaaaacg cgcgcagagc tgcgcgccga aggagccgcg ccatgagcga gggccgtgtc
     1741 cattatacca aagccgacgg catcgcctcc gtcgtcttcg accggcccga agcaagaaac
     1801 gccatgacgt gggcaatgta tggggagctc gcgaccgcgt gcgaggccat cgcatccgat
     1861 accgatgtgc gcgtcgcggt gtttcgcggc gcggggggcg cgtttgtggc cggcacggac
     1921 attcagcaat tcaccgcgtt caccggcgca gaagacggca tcgcttatga acggcgcatc
     1981 gatgcggcca tcgacggcat cgatcgcctg gaaaaaccca tcatcgcgat ggtggaagga
     2041 ttttgcgtcg gcggcggcct cgccatcgca atggcctgcg attttcgcat cgcgtctcct
     2101 tccgcgcgtt tcggcatgcc aatcgcacgc acgctcggca attgtctttc cgtggggaat
     2161 gttgcccgcc ttctcgcgca gtttgggccg gggcgcacca aacgcatgct gatgctggcc
     2221 gaaatgattt ccgccgagga cgcgcatgct tgcggttttg tcgacgttct ggcggcggcc
     2281 gccgacatcg atgtggcacg cgccaaaatg tgcaagacgc tcatggggca cgcgccgatc
     2341 accatgcgtg ccgccaagga agccatccgc agaatccgaa cggagaacct gcccgacggc
     2401 agcgatctta ttcgtgccgc ttatggcagc gccgatttca aggaaggcgt cgaggcgttc
     2461 ctcgccaaac gcgctccgca ttggcagggc cgatgaacag acaaaacgca aaggcggttt
     2521 gcatgacgga caggattctt cgttcggcgg ccatgtccat ggcactttgc ttcgcgttgc
     2581 tcccgcccgc cacggcgcgg cagtggaatc ctgacacgcg cggcgcggcc gccgaataca
     2641 cgcagatcat ccacgccaag cccaatggag agtttgtttt cctatggtgg gccgtaccgg
     2701 aagccttccc gaacgatgcg aacacgcaag tgctgaagga cgtgctttcg cgttacgtgt
     2761 tcgtcggcat cgcgcatggc cgaaacaatg gtacgggtct gtcattcgac aatatccccg
     2821 atctcaggat tgccgatgcg atgggccgcc agatgtctcc gattccggcg aacagcgcgc
     2881 cggaagtcac acaaaccctg aatgccctgc aggcgctgca aagccccatg cggcagggca
     2941 tgcactggtt cgtgttcgag ggcagctcga cccacagctg cacgcccgga aagctgtcgg
     3001 tgcctttcgc gggcgagacc tacaccttcg acacgcccat ccccggctgt cagaaataaa
     3061 actcaggcca aaataaaatc aggccaaaat aaaatcaggc gccgccggcg agccccgcca
     3121 gcacgcgttg cggcgtcagc ggcaattgcc gcagccgcac gccgatcgcg tcatagaccg
     3181 cattcgcaac gccaggccac accgattcgc tcgcgggttc gcccacgcgc atcggtggca
     3241 gatcggaacg gtcgacgagc acgatgtcga ttgcgtcggg aatctcgttg aaagttagaa
     3301 tcggataaga tgcccaatcc aggctcgtga cgcgcgagcg gtcgaatgtg acttcttcct
     3361 tcagcgcgcg gctgatgctc tggatcacgc cgccttcgat ttgatttcgc accgcgttcg
     3421 gattgaccac cagaccgcaa tcctgcgcaa tggtcacgcg ctggacatgg accattccgg
     3481 ttttgcggtt caccgcgact tcgaaaatgc ccgcgacatt cgtatcggtg ttcgagccca
     3541 gcatggccac accacgtccc aacgcaatat cgccatctga gggtttggaa tgcgaggggc
     3601 gcgtttccca cttggctttc tcagccgcgg ctttcagaac ggcgacaccg cgcggacgcg
     3661 gggaatgctt caggcggaat gcgatggcgt cttcaccggc agccgccgcc agttcgtcca
     3721 tgaaacattc ggcgacgaag atattgtcgg gcgaaccgac gccgcgcatg gaaccgaccc
     3781 ggatccgcaa gggcccctga cgatgcgagg cggggccaag ataggtgaac accacacgct
     3841 tgttcggcag gtcttcatag accggttcga aatttcccgc agcacccgag aagccttctt
     3901 cgaggccggg cttggtccag cccgccaatt gcgcggtgac gaggcttccc ggttcgccgc
     3961 catgtttggg ctcgtgatat tcgggacgcg aatagccgga aagaccgaag gtgcgcgcat
     4021 cccaggcgat gatcttgccg tcggcatcca gcccgccgga acattcgctg atgcgcgcgg
     4081 ccttgtaggc ttcccagcca ttttcgtcgg cgcgcgacca ttgcacgcgc accggcttgc
     4141 cgacagcccg cgacagcacg atcgcatcga tgctcacatc gtcggcgccg ttctgaccat
     4201 agcagcctga ggcttcggta tagatcagat gcaccttttc attgggcagg cccaggaact
     4261 tcgccgccgc ttcacgcatc ccataaggcg tttgcgtgcc gcaccagagc gtcacgccat
     4321 cggcccgcac atcggccacc gcgcaggatg gcccgatgga accatgatga tgataaggcg
     4381 acagataggt cgccttcacc gttttggccg ccttgccgag accagccgcc acatcgccgc
     4441 ttttggcgag cacgccgccg ggcgattgct tcggatatcc tgcgctgaat tccggcagat
     4501 tgcgcaatgt cgcgtgcagg tccttcatgt cggggagccc tgcccaatcc gaccaagtga
     4561 tcttcagcgc ctgcgcggcg gcgatggcct gttcctcgcg ccggcagaca attccgacga
     4621 gatcgcctac ccgcaccact ttgacgagac cgggaatatg cgcgaccgag ttttcgtcga
     4681 tcgacatcac agaggcgcca tgagacggcg ggcgcagcac gcggccatgg agcatcccgt
     4741 ccaccttcac atcctgcata taggtgaagg tagcgaagac ttttcccgga atgtcccagc
     4801 gcggctgcga ttggcctacg agcttataat ccttcggcgc tttcggcttc accttgccgg
     4861 tgactgtcag attgaattct ctgccgccga cgagttccgc atagctgatg tgtttcgccg
     4921 cgttcccgcg cgcgctgacc acgccatcgg cagtgaccaa ttgctccgca gggaccgcca
     4981 gccgctgggc cgccagatcg acaagtgcgg cgcgcgcttc ggcggccgcc tgacgaatgg
     5041 ggcgcgaatg ggcggagatg gtgcggctgc cggacgacac gaattgatcc ggcgtgcgat
     5101 cggtatcggg gccgaaaagc cgcacacggt cgaacggcaa gtccagttcc tcggccatga
     5161 tctgcatcag gccggtcgaa cttcccgttc ccaactcgca gcatccggtg aacagcgtcg
     5221 catggccgtc ttcaccgatg gcgatccagg aatcgatctg gttctccggc gggccatagg
     5281 ggccaatctc caaagcagac gcggcgccgg ggtacaacgc gccggcaagg ctgaagccga
     5341 tcatcagcgc gcccgcgccc ttgaggaaat cgcggcgcgg caaagcctct ggaagaagcg
     5401 cgttcatggc ttttctcctc acgcttccga ggcgcgtctg accgcgcgca cgatccgcat
     5461 atgggtgccg cagcggcaga gattgttctg cagcgcggcc ttgatctgcg gcacgctagg
     5521 cttggggttc ttcttcagca gcgcagcggc ctgcatgatc atcccgttga tgcaataacc
     5581 gcattgcacc gcctgttctt cgacgaacgc ggcctgcagc ttgtgaggct tctcgctcgt
     5641 gccgagacct tccagcgtca caatctcatc gcgcgcgccg atcgaggaaa gcggccgcat
     5701 gcaggaacgc accggcgcgc cattcacatg cactgtgcaa gacccgcatt gaccgagccc
     5761 gcaaccgaat cgcggaccgt gcaggccggc atcgttgcgc aggacataga gcagcggcgt
     5821 cgacgggtcg tcgacggtcg ccgtcacact cttgccgttt acgcgcaggg ttgtggtcgt
     5881 tgccatgatc ttcgatccgc tggaactatc gatgcgtgta tattacaccg gccattctgt
     5941 gcatgaccat gcgatcacgc ggttttttct gcttccacca ccgcatcgcc gcgcgccgga
     6001 ctaggccccc ccgccaaggc ggatttgagc aagacgcaga gtccgcccaa caacggcgca
     6061 agcgccatca cggccaggat agggccgggt gaaaatccga ggccgagcat tttgctgacg
     6121 gcatagggac tgcagacctg cccgaaccgg cccatcgcaa gcacccagcc tgtgccggcc
     6181 gaacgcatgg ccgtgggata ggagatcgcg gcgagcgcga tcacgcccga tgatgcaacc
     6241 gatacggtaa gcccgagcat catcatgatg gcgcccgcgg caatcggcgt atccgcaaca
     6301 agcccaaggg cggcgagcac gaccgcacca ccggcaaacg gaagcgcaat tccaagcacc
     6361 ggaccgaaac gattcaacaa ccaacccgcg atcgccatgc cgcatgtggc aaccaaactg
     6421 taaacggcga cgaggaaggc cgatgccgaa agcgccatgc cggcttcacg gaagatggtc
     6481 ggtgtctgca atacgatgac cgtcgtggtc cagaacccga aataaagcat tagccccaga
     6541 aggatcgtgc ccgccgcccg gccattggca aagagatcgc gcagtggggc tttgggcgcg
     6601 gcaaacgttc tggcggcaat ggtcatcgct tcgggcaaat cgggatcgag ccggcgcgcc
     6661 agatgtgttg cgcgctcccc gcttccgcgc gcggcaagga agcgcagcga ttccggcatg
     6721 aagagaagca gcagcagggc gacgaaaagc gtcgggatgc cgcccacata gaacaccatc
     6781 gaccaatgaa aatgtttcac gacgtaggag gtcatgaaac cccctatggc attgccgagt
     6841 ggataaccgg cccacagcag gcttaccagc atggcgcggc tgcggtgagg cgcgtattcc
     6901 gcggccatgg tgatgaagca gggcgttgcg cctcccaagc ccagacccgc ggccaggcga
     6961 aaggcgacaa gctccggaaa ggagccagca aacacggtca gacaggtgaa gatgccgaac
     7021 aggagcgtgg tgaacaccag tgtcggcttg cggccgaagc gatcggaaat cgggccgaag
     7081 gcgaaggcgc cgatggccgc gccgaacaat cccgccgaat aagcaggcca gaaatcggcg
     7141 ggagcgatct tcaaaccctg tgcgatcaag ggccccgcaa cgccgatgga ttggctgtcg
     7201 atcgcatcga gtaccgtcac agccgcgcac aggatgaaga cgcgaatctg cagggcggaa
     7261 atcggccttt cgtctatcag cctgtcgaca tcgacctgca tgtccggtcc cgtagccttc
     7321 ctcaaacgcg aggcgcaaaa acgctatcat gccattcgcg gcgccgcact gtcattcacg
     7381 gtgccgtgca atcagcgcgc gcagatattc ggcagccgcc gcgcctttca aagcacgctg
     7441 ccacatgcgc tcggcggcgc gatgatgcag cgccaaggct tcttgcgccg aggtgatcat
     7501 ggcgacacca acgcgcacat tgggttcctc ccccagacga aagggcgaga gcgacagcaa
     7561 ttgccggtcg ggttggcgga aaatctggaa gccggtatgg ggcagcgttt cgggcactat
     7621 cccgatctgc acgccgatgg gctcatcctc catgagcttc gcgaaatatt ccacctcgct
     7681 gctggcaagc attcggcgtt cgcgccgcag cttttcgggc agatcgaacc ggcccaccat
     7741 cccgttgatg aggaagcgcg cgatctcgga agccgcgatc agattgacga tggcgggctg
     7801 ccgccggcga taggtttcct tgcgctgctt cagcaccgcc atgatctctt cgacttcggc
     7861 ctcgccgcgc gcgcgatccg cgccgatgtc ggaaatgctt tccttcagaa gctgaccgag
     7921 cacggcattg aaggcatcgg aggcaagcag ataggaaagc gggcccgcga gcacgatgat
     7981 atgctcagcc gtttcctcga tctgacgcat gcgctcgaaa taggtgacgg ccgaggcgat
     8041 atattcgaca cccacgccca gcaaagtcgg cagagagact tccagcagct ccgccaattt
     8101 ctcgagcgtc tcgattttca ccacctcgcc cttttcgaag cggtagagag cggtgcgcga
     8161 aatcccgatg cgctttgcca cctcttccgc cgagaggccg gagccgagac ggaatgcctt
     8221 caggcgattg ccgatatcgt caaagcggag cggcataagg tctccgtggc ctcacggagt
     8281 gtccttgatg gctttcagac gttgcgccgc atcgagggcc gccgccacga tgccctgata
     8341 tccggtgcag cggcaaagat tgcccgagat cgcgatgcgc acctcatgct cggtgggatc
     8401 gggattgtcg cgcaggaatt cgatcagcgt cgtcagcatg cccggcgtgc aaaagccgca
     8461 ttgcaggccg tgattgtcgc ggaaggcttc ctgaagcggg tgcatcttat cggccgaagg
     8521 cgcgatgcct tcgacggtga ggatctcatg tccgtccgcc tgcaccgcca gcatcaggca
     8581 ggaccgcgcc gacagcccat ccagcaaaat cgtgcaagcc ccacacacac catgctcgca
     8641 tcccagatgc gtgccggtga gtcccaattg ctggcgcata aaatcggcca acgtgaaacg
     8701 gacctccacc tcttcgtcgt aggtcttgcc gttgatggtg acggcgatgc ggcgtttctc
     8761 gctcatgcgc gtcccacccg ttccatggcg gccacaagcg cgcggcgcga catcaccgtt
     8821 gccaggtgct gacgatagga agccggcgcg tgcacatcct cgatggcgtt caccttgcgg
     8881 cactcttcgc aaagacgccg gaacaattct tccgacggcg tttcgcccac cagcgccttc
     8941 tccacctcgc gcatgcgcac cggcgccacc gcgacaccgc caagcgtgag cgaaacacgc
     9001 gcgattctgc cgtttgcgcc cagttccagc agcgcggccg ccgaagcgat ggcgaaatcg
     9061 ccgtgacggc gcgcgaattc gacgaagcca taaccgtgcc ctgcgggcca gcggggaaac
     9121 cgtaccttgg tgagcacttc gtcggcttcg agcgccggcg tcatgaaggc gacaggaaat
     9181 tccgcgaacg gaatctcacg tgtgccgttc gggccggccg ccgtgacgac ggcatcatgg
     9241 gcagccgcca tggtgaccag ttccgcggat ggatcgagat ggcagagcga gccgccgata
     9301 gtgccgcgat tgcgcgtctg gcggtggccg acattcagga tagcctcatg gaggatggga
     9361 aaacgcgtct tcacaacgtc ggaaaattcg atgtcgcgct gccgggtcat cccgccgagt
     9421 tcgagcgcgc cgccctcctc gcgcagatag gcgagcgcgg cgatcttgtt gaggtcgatg
     9481 agatgatcgg gaaggacgta gcggaaattc agcatcggca tcagcgattg cccgcccgcc
     9541 agaagcttcg cgttttcgag acggctcagg agcccaacgg cctcctccac cgtgcccgga
     9601 tcgtaatagc gaaaaggcgg cggcttcatc ccaaatccta ttcagctgcg cggcaacaac
     9661 ttagcgaatt tcgcgcggat tctctcatcc aattctttgg agttgcgcac aaccgcgggg
     9721 aacgtatccc ggcgcgccca ggggcggcag gcatcgatca ccacgcgtgc gttgcgccgg
     9781 tcggactcgc tgtacaccat gggatcgagc acggtgctcc agcagccctt gatgacttcc
     9841 agatcctcgc gcgggtcgaa acgggtgcac atggcccaca gcaccttgtc catgtcggcg
     9901 gggtcgatgt catcgtcaac caccacgacg aagcggttgg cataggcgcc cgcatgacat
     9961 tgcgaggcga tcagccccgc ctgcttggaa tgccccgcat agagctgccg aatcgcgacc
    10021 gtcagccaca gccggctgcc gcccgcctca tgcgcccata cccccttcac ttcggggatg
    10081 ccggcggctt cgagctgatt ccacaccgcg cccgagcgat aggtgccgcg atagaaggaa
    10141 tcatcgtccg gcggcacgcc cggaatcgcg cccatcagga tgggattgtt gcgatgcatg
    10201 aaggtctcga tgcggatggc gggctccttt ttcagccccc cggcgtaata gccggtccac
    10261 tcgcccagcg gcccttcctt gatcaggtcg tccggataca cgaaaccttc aaaggcgatt
    10321 tcggcatggg caggaatcgg cagccctgtg cgggggcccg cgatcacctc cacgggctcg
    10381 ccaagaaggc cgcccgccgc atcatattcg ttcttgccat aggggatttc gaggcctgcg
    10441 atcatgaaca gaagcgggtg catgccgcac accaccgcga tcgggcaggg ttcgcccttt
    10501 tcgtgatagg agcgcatgat cagatcgccg tgcttgccct tggagcacat gatagaggcg
    10561 acaccgggct catgcgcctg cacgcggtag gcgccataat tcacccagcc ggaatcgcga
    10621 tgcttcatca ccaccatgtc gccggtaccg atgtagtaac cgccgtccag ctcgtgccat
    10681 cgcggcacgg gaatcttgaa gatgtcgatg tccttgcctt cggcgacgtt ttcgaacaaa
    10741 gcgccggtct tcaccgtttg cggcggaatg gacttcgcct ctttcatgta gcggcgccaa
    10801 taatggacga gatcgatggc gctggaatcg gccggattgc cgagggtgag atggatgcgc
    10861 cggatggacg tcagaatatt ggcgatcacc cggtggccgc gcggatagcc cgggacgtcg
    10921 tcgaacagca cggcgggatt gccggtcttg cgctgataga aatcgacgat gccgccgatc
    10981 tcctcctcgc gctccgcacc cgaaatgtgc tggatctcgc cagcggcttc catcctggcg
    11041 atccagcttc gcaaatcctg cgtcgtcaac gcgtcctccc tgagaccgat actctaaacg
    11101 tgatcgcggc cgaccgcatc ggcgactgac agataagcgt cacgcttcaa acaatccgcc
    11161 aactcgcgct tgatgcgatt tgcgagctta ggcccttcga acaccagcgc cgaataaagc
    11221 tgcaccagcg aggcgcccgc cttgatcttg gcataggcat ccgcacccga ggccacgccc
    11281 ccgcaaccga tcagcgggat acggcctttg gtcaagcgat aaaggctgga aaggcattcc
    11341 gtcgagggct gaagcagcgg cgcgccggaa agcccgcctg cctcgaagcg ctgcggattt
    11401 ttcaagctct cgggccggtt gatcgtggtg ttgccgacga taagtccgtc aacgccggtg
    11461 gcgagcgcca cttcggcgat atcggccaat tgttcgggcg agagatcggg gccgaccttc
    11521 gccagcaacg gcggcttttt cccggtggga gcattgcgcg cccgcgcgcc gagcacgcgc
    11581 ttcagcaaat cctcgatggg cgcgcgtgcc tgtagagcgc gcaagccggg cgtgttgggg
    11641 gaggaaatgt tgaccaccag atagtccgaa agaccggcga tcgtctcgat ggcggcaaca
    11701 tagtccgccg cgccatcggc cgtgtcgcgg ttcttgccga cattggcgcc cacgataccg
    11761 ggattgcggc ggcggtgata acgcgcggcg agccgcctgg cgaaaccttc cacgccgccg
    11821 ctgttgaacc caagccggtt gatcaccgct gcgtcctcat ccaggcgaaa caggcgcggc
    11881 ttgggattgc cgcgctgtgg ttggggcgtc accgttccgg cctcgacaaa accgaagccg
    11941 aggcccagca tcgcatcgac cacgcgcgcg tccttgtcga agccggcggc caaaccaatg
    12001 gggttggaga attccaggcc catgactcgt gttttcagga tcgggtcgtc gggctcgcgc
    12061 cgcgcgccca ctagcccatg ttccagcgcg aacagcaccg cgtgatgcgc ggtttcgggc
    12121 ggcagcgtat agagcgcgga aagcgcgacg cgatccatga cgccttttcc ttttttcgct
    12181 acgatttcgt tgccccgcca aacagacggc ggaagaaact ctttaagacc atcgccagca
    12241 gcgcaaaacc ggagatggcc ttgggcttcg gcggctcaac ctgttttcca ctctgcgcga
    12301 tttccggtcg cgcaggttcg cgcgaagcgt cgagttgcgc tttcaaggaa ttcgcgaact
    12361 gcccgatcaa ttgctgcgcc acatcctgga tcatgcccga cgcgcggcca tattgcgcga
    12421 ccgcaccgga cagcgtcagg tccgtcttga cgagcacacg cgtccccagc gccgagggtt
    12481 cgagatggaa atcgacattc gcgttggcgc cgccgcggcc cttggaatcc ttgccttggg
    12541 ctttcacgcg cgcggcgtgc gccacatcgt cgatgtcggt gaatttcacc tgaccggcga
    12601 aggtcagcgc caccggaccg agcttgaccg acaccttgcc gctgtaggtg tctttatcca
    12661 ccacacccgt cagttccgcg cccggcatac agggcgcgat gcgcggaatg tccatcagga
    12721 ccttccaggc atcggccggc ggcagcggca cttcgaaggc gttgttgatt tccatgctgc
    12781 ctctatctag atgccaagcc gttccgcagc ccagcgcgca gcggacagca aaatctcgtc
    12841 ggcgtaaagc tgtcccacaa gctggatgcc gatgggaaga ccatgcggac ccttatgcgt
    12901 gggaagagtg atcgccgggg tgtgcagcgc ggtccagaat tcctggaagc gcgtgtcgcc
    12961 ggtggaggcg aggccacgcg gcgcttcgcc agtgacgcaa ggcacgagca gcacatcgaa
    13021 atcgccaaat agcgccggaa gcatgtcgcg gcattccttc atgcgcttga gcgatgcgac
    13081 gtagcgcgca tgcggaatgc tgagcccctc ctcgatctgc cgcgtgaggc ccgggctgat
    13141 gagagcgcga tttgtgttcc attcatgggc catggaccgc gcccgctcga caggattgac
    13201 gatggtccgc gcctcggcga gggcattgaa attctctggc agcacggcat cgcgaacgat
    13261 agccccggcc gtttcgaacc gttgcgcgca atcttccacg gcgcgtttgg attcagggag
    13321 cgcatgctcc cagagatggg tgcggcaaag cccgatgcgg ggcggcgtgt cgcggcgcgg
    13381 actgagcggg cgcgcgatca gcacgctcgc aatgagatcg gcatcgtcga cgctgcgcgc
    13441 gagcagaccg atggtatcgc ggctttcggc ggcgaatttg atgccgccgc gcgcgatcag
    13501 attgtaggtc ggcttatagc ccaccacgcc gcaaaacgat gcggggcgca gaaccgaacc
    13561 gccggtctgc gtgccgtaac cgacagcgca catcatgtcc gcaaccgcgg cccccgaacc
    13621 gctggacgag ccgccgggcg tgcggtccag atcatgggga ttgcgcgtga cgcccggcgt
    13681 cagcccggcg aattcacagg tcacggtctt gcccaggatc accgcgcccg cgcgacgcgc
    13741 cagcgccacc gatgaggcat cggcaaaggt gcgattgcca tggtagatcg acgaacccat
    13801 atcggtcggc atgtcgaagg tgtcgaaaat atccttgatg ccgagcggca cgcccgccaa
    13861 cacacctttg acggcgcccc gatcgatcgc gcgtgcctga ttcaacgcca gttcgggatc
    13921 gacgaacgcc cacgccttga cggcgccatc gcgttcttca atgcgcgcca ggcaatcgcg
    13981 cgtcacggct tcggcggtga aggcgcctgc cgcgacgccg cgcgcgatct cgcaagccga
    14041 aagcgtgtta aggcggcccg ccgccatcag cctctcgcgg ctttgccggc ggtggagccg
    14101 gcgattttgc cgaacaccga acccgaggtg aggcccgtac cgcccggata gttgaaatag
    14161 aaaatgccgc cgacgagttc gcccgccgca tacaggccgg gaatgggttg gtaatcgaga
    14221 ttcatcacct ggccgtcttc ggcgatgcgc aagcctccaa aggtgaacgt cacgccgcag
    14281 gtgaccgcgt agccttcgaa cggcggggtg tcgatagtgt tggcccaatt ggatttggga
    14341 acggcaaggc cttgcgttcc acggccgtcc ttgatgttcg ggttgaatga aacgtctgtg
    14401 ttcaccgcgg cattgtattc ggcaatggtt ttgacgaatt gttccgcatt cacgccgtca
    14461 agttttttgg ccaattcctc aatcgtgttc gccgtcacct tggtgcattg gcggatgttg
    14521 tattcgtcgc gctgcagatg cttggtcttc ttgtcccaga tctgccaggc gaactggccg
    14581 ggttgttcca gcaccacccg gccatatttg gcataggtgt aattgcggaa atccgcgccc
    14641 tcatcgacga aacgttttcc ggtcgcgttc accagcagcg caaacgggta ggagtgcttc
    14701 tggaactggt cgcccacggc gagatcaccg aattccggcg cgttcatttc ccattgcacg
    14761 gcatggcagc cggaccaatt gccataaggc gacgcaccga tggccagcgc catgcggatg
    14821 ccttcgccat tgttgaagcg cgtaccgcgc accttcgcca ggtcccaacc cggtccgagg
    14881 tagcgcgtgc gcatttcggc attggcctcg aaaccgccgc tcgcgagcac gaccgcgcgc
    14941 gccttgagtt cgccgacctc attgccgtgt ttgtagcgca ccccggatac gcgcacgccg
    15001 tcatagagaa gatcggtcgc gcgcgtcgaa tagcggatgt cgatgccgtt cttcttgccc
    15061 gaggcggtga gcatgtcgac gagacccggc ccgccgccga tcgtctcaag cgtcaggccg
    15121 ccccagaatt tgaacttgcc atcgatcttg aaggcctgcc gcccatagat cggaacgaaa
    15181 cggatgccct tctcgcgcat ccagatcagc gtcggcaggc tctttgtcac cagcgcttcg
    15241 cagagattgg gatcggtgcg gaattgggtg acgcgcgcca tgtcgtcgaa gaactgatcc
    15301 tgcgtatagg taccgaaatc ggacgtgtcg atttcggcct ggctgagatc gggcacgagt
    15361 gcggtgatgt cctccacgtc attgtagacg acccgcatcg cccccgccgt gaagcggctg
    15421 ttgccgccgg attcatcttc gggcgccgct tccagcatca gcaccgaagc gcccgcttcg
    15481 cgcgcggcca gcgcagcaca gaacgcggca ttgccgccgc cgacgatgat gacatcccat
    15541 tcgttcatct tctcaagccc cggcgccggc cgttccccca tgtgctttcg accagccgcg
    15601 cg
//
LOCUS       DASYEG010000247        17811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3135176, whole genome shotgun sequence.
ACCESSION   DASYEG010000247 DASYEG010000000
VERSION     DASYEG010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 17811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17811
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3135176"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VGM72_10715"
     CDS             <1..293
                     /locus_tag="VGM72_10715"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4265781.1"
                     /translation="QNWNYPIGHVDPASNSTALAEKWKPWIETTIELFGVQRCMFESN
                     FPVDKTAASYDTIWNAFKRITASASADEKAALYAGVARRVYRLPVQTASATP"
     gene            complement(343..1179)
                     /locus_tag="VGM72_10720"
     CDS             complement(343..1179)
                     /locus_tag="VGM72_10720"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEY4265782.1"
                     /translation="MPSLDQAACETLVRREDPDRWLAALFADAQPRPHLVALYAFYYE
                     VAKTADSVSQPVMGQIRLQWWREAVEEIYTGHARRHEAVQVLAETVRSHDIPRALLDA
                     MIDAREHDLEETPFTDWVSLETYADATCGNLMWLAARILGAGPALEDAAREAGIAYAL
                     VGLLRAFGFHAARRRLMLPVAALHEIGLSQEQIFSGTADAKVTALFARTVERALDHLN
                     RVRASRIPRKFLPALLPAALVPGYTKILTRPGFNPFRDVAELPVHRRQIGMLAAMARA
                     RV"
     gene            complement(1294..1911)
                     /locus_tag="VGM72_10725"
     CDS             complement(1294..1911)
                     /locus_tag="VGM72_10725"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEY4265783.1"
                     /translation="MIATILSNLRNTVIAGFVLAFIVFLIYLTQTEFNNFTFWPFLIR
                     WFHIICGVMWIGILWYFNFVQTPMMPKIPDELKGGVSRFIAPEALFWFRWGAMGTIFF
                     GIILAALNGYLLQAYTLDAIEGFIDLRVILIGIGMWLGTIMWFNVWFIIWPNQQKVLN
                     IANAYPDLPAAEKAKAARSASMFSRVNTMLSIPMLFCMAAAPHLA"
     gene            complement(2021..3646)
                     /gene="secD"
                     /locus_tag="VGM72_10730"
     CDS             complement(2021..3646)
                     /gene="secD"
                     /locus_tag="VGM72_10730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY4265784.1"
                     /translation="MIYIGRWQTITVLLILLLGVIFAAPNFLSERVRGVLPGWVPSST
                     INLGLDLQGGSYLLLEVDMPGVIKERLETIRGDIRAALRKVRINYTGLDAARESVTLH
                     VTDSTQLDAARKILKDIASPPGTFLGLGAAEYDMTDDGTGNFTFRISPAYLRQMQTQI
                     VTQSIEVVRRRIDALGTREPTIQQQGDDRILVQVPGLKNPEELKRVLSTTAKMTFRLV
                     DVSGNVQDALPAPAGKGRVPPDDELLYETDRAGQPTQPYLVQRRVMVSGDRLEQAGSG
                     FDPRDGRPVVNFRFDTRGAREFGDVTKANVGKPFAIVLDNKVISAPNIQEPIIGGSGQ
                     ISGNFTSETANDLAILLNAGALPAALSVIEERTVGAELGADSVQAGKLASIGGLLAVV
                     VFMIATYGLFGLFATVALGVNMLLLIAVLTALQATLTLPGIAGMVLTMGMAVDANVLI
                     YERIREEMHSGKTVIAAIDAGFRRATATIIDTNMTHVLAAAILYAVGTGPVRGFAVTL
                     GLGVITSFFTAVMVTRLIVVTWLRQRRPKALVL"
     gene            complement(3688..3984)
                     /gene="yajC"
                     /locus_tag="VGM72_10735"
     CDS             complement(3688..3984)
                     /gene="yajC"
                     /locus_tag="VGM72_10735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEY4265785.1"
                     /translation="MNFNDPLVSTMVPLLLLFGIFYFLLIRPQQQRVKAHQQLVEGVK
                     RGDTVVTSGGMIGRVAKVKDDGEIMVEIADNVQVRILKSTLTEVRSKGDVKGES"
     gene            4085..4960
                     /locus_tag="VGM72_10740"
     CDS             4085..4960
                     /locus_tag="VGM72_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019977368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4265786.1"
                     /translation="MTRDFTKIEDLLARIAAALEQRMPRPPEDIQTVLGASDVFVWEA
                     DTAQLVAVRQTTGLPLGLLKGIDAPRDTLFENTRRFAGGLPSNNALLWGARGMGKSSL
                     VKAVHAEVNAKLAPSNRLVLIEIHREDIVSLPQLLRLLRGQKRQFLLFCDDLSFDRDD
                     TSYKSLKAALEGGVEGRPSNVLFYATSNRRHMMPRDMMENERSTAINPSEAVEEKVSL
                     SDRFGLWLGFHNCAQDEYLAMVESYAAYFQLPLAADALRARALSWAAGRGARSGRVAW
                     QFIQDIAGELGKPLR"
     gene            complement(4957..5847)
                     /gene="surE"
                     /locus_tag="VGM72_10745"
     CDS             complement(4957..5847)
                     /gene="surE"
                     /locus_tag="VGM72_10745"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEY4265787.1"
                     /translation="MSRVVLRSRKKQLRILVSNDDGIHAPGLAVAEKIARSLSSDIWV
                     VAPETEQSGASHSLTLTLPLRLRQAGPQRFALSGTPTDCVMMAGIHIMKDHLPDLVIS
                     GVNRGFNVADDVTYSGTVAAAMEGAVLGIPSIAMSQAAGGDYEEDVLFACAEKHGPPL
                     VRRLVEIGWPEGVLLNVNFPALPPGKVKRVMVTAQGQRDQSILRVDERSDARGRTYFW
                     VGFKRIFGDPERGTDLHAIAQGCISVTPLHMDLTELREQQRLSGLLETSLTSLARAKP
                     ALKSKRKKPAAARLRTAAKS"
     gene            complement(5844..7262)
                     /gene="serS"
                     /locus_tag="VGM72_10750"
     CDS             complement(5844..7262)
                     /gene="serS"
                     /locus_tag="VGM72_10750"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY4265788.1"
                     /translation="MHDIKAIRDDPAAFDEGLKKRGFAPISPEILKRDEILRNIKTIL
                     QQSQARRNEASKLIGQAKAKKDEAQATALMGEVAVIKETIQDGEEKERRFQKELDDLL
                     AGIANIPADDVPVGDESANKEIRRWGAKPDFAFTPKEHFDLGEGLGQMDFEAAARMSG
                     ARFVVLKNQLARLERAIAQFMLDLHTDQVRENNTSEKLEIGGYAEISPPLLVRDHAMR
                     GTGQLPKFERDLFFASSYVEFEEKPVGSADLWTNNAGEFKVTPRFGPTKGMLPPSWLI
                     PTAEVPLTNLVREQILDGAQLPLRFTAYTPCFRAEAGAAGKDTRGMIRMHQFSKVELV
                     SITTQEQSAAEHERMTQCAQEVLKRLNLHHRIIVLSTGDMGFGARKTYDIEVWLPGQN
                     AFREISSCSNCGDFQARRMNARYRPPGEKNTRFVHTLNGSGLAVGRTLVAILENYQQA
                     DGSILIPDALKPYMGGVDRIAR"
     gene            7349..7726
                     /locus_tag="VGM72_10755"
     CDS             7349..7726
                     /locus_tag="VGM72_10755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEY4265789.1"
                     /translation="MSEDEHAIRALIADWIAASKAGDLATVLGLMSDDVVFMVPGRSF
                     GKQEFAAASAGMKGMDFIGESEVLEIEVLGTRAWCRTHLTVTVTPPNAAPVRRSGYTL
                     SILAKNAGGKWVITRDANLLAAG"
     gene            complement(7731..8555)
                     /gene="tatC"
                     /locus_tag="VGM72_10760"
     CDS             complement(7731..8555)
                     /gene="tatC"
                     /locus_tag="VGM72_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY4265790.1"
                     /translation="MASPSSDEEAELESSKAPLLEHLIELRNRIVRSVIAFLVCFIFC
                     FAFSKPIFAFLTRPISDALAGQANRQMIFTALYEQFFTYVRIGMFGALCLAFPYMALQ
                     LWLFIAPGLYKHERKAFWPFLLATPVMFVTGAAFVYYLMLPRVIQFFVGEEIPAGPGQ
                     IAVQLAPRVGEYLTFVMTLIFAFGLCFELPVLLVLLGRVGIVSSKALSSARRYAIVGI
                     VALAAVVTPPDVFSQLSLSIPMIGLYEVSILCVKLIERQRAKAEAAAAGPGDIQPT"
     gene            complement(8648..9124)
                     /gene="tatB"
                     /locus_tag="VGM72_10765"
     CDS             complement(8648..9124)
                     /gene="tatB"
                     /locus_tag="VGM72_10765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEY4265791.1"
                     /translation="MFGIDGSEYLVILIVALVVVGPKDLPKLMRKVGQWAGRARAMAD
                     QFRRSFDDMARQAELDELRNEVNKMKAQAVEAAQPMTDKDYAALGIEFPKDPLGIQST
                     IEEVGAAPPIFDTPPAAAPVPAGPIPASAPQPVETGAEAAAEEAAKATQPVTGTNG"
     gene            complement(9228..9434)
                     /locus_tag="VGM72_10770"
     CDS             complement(9228..9434)
                     /locus_tag="VGM72_10770"
                     /inference="COORDINATES: protein motif:HMM:NF001940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY4265792.1"
                     /translation="MGSFSIWHWLILIAVVALLFGGKGKISGLMGDFAHGIRNFKEGL
                     KDPNDPSPPADKVEAKKADEVKQG"
     gene            complement(9539..10687)
                     /locus_tag="VGM72_10775"
     CDS             complement(9539..10687)
                     /locus_tag="VGM72_10775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4265793.1"
                     /translation="MRYAFAKECLLNAASQISDETCTAPADGLSLRNVTRRFGTRIAV
                     DGVTLDVKRGEVLCLLGPSGCGKSTSLRIAAGLERADSGLVFVGGRLVEGQGRHEPPE
                     TRRVGLMFQDYALFPHLTAKRNVAFGLAKLARAEREARVDDELARVGLTGLRDAFPHT
                     LSGGEQQRVALARMLAPKPDVVLMDEPFSGLDSALRDDVRCTTLKRLREEGAAVVMVT
                     HDPDEAMRVGDRVALMRAGCIVQTGTPAEIYRHPADPQAAALFGGANVFHARVTKGCA
                     ASPFGQTSAKSVAEGEWAEILYRPASVRVADTGVQARVLAVRPYAGQLEIEAAFFTNA
                     LPEGVEVPISVRAAAPAGTGLVPGSVVCLAAEPQEAFVFPCRNEICRK"
     gene            complement(10936..12450)
                     /locus_tag="VGM72_10780"
     CDS             complement(10936..12450)
                     /locus_tag="VGM72_10780"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HEY4265794.1"
                     /translation="MSLTDVQFKASSRRIGRYQPIAIVDIGSNSVRLVIYEGPWRHAA
                     ALHNEKAICAIGRNMVSTGNLDAEGMIFAIETLARFRELCDGHNVRDVGAVATAAARD
                     ATNGRDFIRRAEKTLGQPIRILSGEEEARIAAEGTLAGLPDADGLVADLGGGSLDMVT
                     VKDGKTGAAATLPFGPLRLMDVAGANLNKARNVVEKGLGKLDFAKSLKGRALYAVGGI
                     WRALAHVDMDERDYPLRVLHHYVIPAGRAVKLCRVVSGLSRKSLEKMRSVPKRRAEAL
                     PYGALVMEEMIQGLGLKEVVVSAFGLREGVLQRKLKPEEAAKDPLIAFARDLNERESR
                     LPGHAPELFRWMTPLFPKEGAAERRIREAACLFSDIGWRRHPDDRAMGTFVQVLRGAY
                     GGADHHERALMASAAYYRYAGDDDFPEETRVASLLGADGAVKAQQIGLAARLAFGLSG
                     AVEGELPHAKLMLTKDTLALELPLAKKALLGETAHKRLADLADVFGRKPQTALV"
     gene            complement(12468..14705)
                     /locus_tag="VGM72_10785"
     CDS             complement(12468..14705)
                     /locus_tag="VGM72_10785"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003919.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEY4265795.1"
                     /translation="MNKATLREIAASGVTATGPEAVPDMPASGAAATLIPGLSLQSPG
                     RFINRELSWLAFNRRVLEEAQNLRHPLLERLRFLSISASNLDEFYMVRVAGLKGMVNA
                     GVASLSDDGLTPAQQLSRVDQMAAELILDQQKTWRELRVALVNSGIAVIEPDQLSVED
                     RAWMDQEFSQQIFPILTPLAIDPAHPFPFIPNLGFVMAMRLTRPMDGKALTALLPIPS
                     QLQRFVRLPDREGKARFITLENLIGLYLDRLFPAHAVTAAGVFRILRDSDIEVEEEAE
                     DLVRLYETLLKRRRRGSVIRLKCSASMPPDLMRFIADHLYMGQREPFVVDGLLGLDET
                     KQLILDERPDLQFEPFTSRFPERIRDHGGDCFAAVKAKDLIVHHPFESFDVVVQFLRE
                     AAQDPDVVAIKQTLYRTSKDSPIVRALIEAAEAGKSVTALVELKARFDEAANIKWARD
                     LERAGAQVVYGFIELKTHAKVSLVVRRESGKLRSYVHFGTGNYHPLTAKIYTDLSLFS
                     ADPALGRDAAQFFNYVTGYAEPSHLEKIAISPLTMVNRLMDYIEAEIEHARAGRPAAI
                     WAKLNALVDARVIDALYRASQAGVQIDLVVRGICCLRPGVAGLSDNIRVKSIVGRFLE
                     HSRIVCFGAGYGLPSPKAKVYISSADWMTRNLDRRVEAMVPIDNPTVHQQVLSQIMVA
                     NLKDQAQSWVLRADGSYARNPAFDRPDAFSAHTYFMTNPSLSGRGRALKVDTPPNVAG
                     AIRGA"
     gene            complement(14817..15500)
                     /locus_tag="VGM72_10790"
     CDS             complement(14817..15500)
                     /locus_tag="VGM72_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265796.1"
                     /translation="MFGQLALPFGVKSAHGRDDFIVAPCNEPAFQFIRRWPDWPSYAA
                     ALYGPPGCGKSHLAAIWRETAGAQIIDAYALRPENLDAQDSGTALVIEDFDRAPPGET
                     RDRALLALFERPKGWLLLTARTRPAAWQSPIGDLASRFSALIGFAMWAPDDVLLAGLI
                     AKHFADRQLEVPEAVIRRIVIQLERTPAAIAAFIARLDEKALGEKRAVTERLALELLE
                     SETPSGSRD"
     gene            complement(15526..16098)
                     /locus_tag="VGM72_10795"
     CDS             complement(15526..16098)
                     /locus_tag="VGM72_10795"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY4265797.1"
                     /translation="MMFMLRHLPNMITTLRLAAAPTTAVLLVAGHFSAAFSLFAFAGL
                     SDAADGFLAKRFNLASRLGRLLDPAADKALMIAAFVTLAILDDVPAWLTVTVIARDLL
                     ILLGLAWALALRAPIAVQPLFRGKVCTALQVIYIGWHLAALAFDLPVDASAPADAYLV
                     AAFAWVSGFAYLAVWLKAMRTVNVQEGRRA"
     gene            complement(16095..17156)
                     /locus_tag="VGM72_10800"
     CDS             complement(16095..17156)
                     /locus_tag="VGM72_10800"
                     /inference="COORDINATES: protein motif:HMM:NF021367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HEY4265798.1"
                     /translation="MLRLSLLVLAAGFILRAGAVQAAPAPVPDIFTVAGIKVDATAES
                     ATAARDLAMAEGRPLAWTKIYRRVTATANWGKQPKLDDNAMLRMVRSFEVSNERRSTT
                     RYLAEVTYRFNAAAVRALLRQSGIAYTETRARPSLVIPIVAGTAGVDPMSAWAMAWKD
                     PALQQGLVPFVLPAADAQDMEILSRPDLAQADWASLAPLARRYNATEAIIAIASEDAK
                     TVQMVEISATARIAASFAYANSTFAADAEVVAERAYETWKTRSAVNFATRGQLTADVQ
                     FETLDDWAKIRKQLGAVKAVADMDVTGLALHEAEIQLVYFGRPEQLRDALAQQNLLLA
                     NAGGQFTLQLAGRSAANAP"
     gene            complement(17285..>17811)
                     /locus_tag="VGM72_10805"
     CDS             complement(17285..>17811)
                     /locus_tag="VGM72_10805"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY4265799.1"
                     /translation="GAAGDIAPLMEGAGRILAAEGGPRVAVFDITGWDTHSNQGAGEG
                     QLARRLQLLDEGLAALKASLGPAWQKTAIVAASEFGRTVKVNGTGGTDHGTAGIGFMF
                     GGAVAGGNIHAEWRGLKPSVLRDGRDLPAQTDTRALFKAALAEHLGVSKQALEEKIFP
                     ESATAAAPKGLIRA"
BASE COUNT         3479 a         5563 c         5542 g         3227 t
ORIGIN      
        1 gccagaactg gaactatccg atcggccatg tcgatcccgc gagcaactcg actgcgctgg
       61 ccgagaagtg gaaaccctgg atcgagacga cgattgagct atttggcgtg cagcgttgca
      121 tgttcgaaag caatttcccg gtcgacaaga ccgccgccag ctacgacacc atctggaacg
      181 ccttcaagcg catcacggcg agcgcctcgg ccgacgaaaa ggccgcgctc tatgccggcg
      241 tcgcccgcag ggtctaccgg ctgccggtcc agacggcgtc ggcaacgccc tgaggcttgt
      301 ggcgagcgtc acgttcgacc gatcggtgtc ttttcgaata attcacacgc gagcgcgcgc
      361 catcgcggcc agcatgccaa tttggcggcg gtggaccgga agctccgcga cgtcgcggaa
      421 cgggttgaat cccgggcgcg tcaggatttt cgtatagccg gggacaagag ccgccggaag
      481 caatgccgga aggaatttgc gcgggatacg cgaggcgcgt acgcggttca gatgatcgag
      541 cgcccgctcg acggttcgcg caaataaagc cgtcactttg gcatccgccg taccggagaa
      601 aatctgttcc tgcgaaagac cgatttcatg cagcgctgcc acaggcagca tcagtcggcg
      661 tcgcgcagca tggaaaccga acgcgcgcaa caatcccacc agcgcatagg cgatgcccgc
      721 ctctcgcgcc gcatcctcga gcgcgggtcc cgcacccagg atgcgcgcag ccagccacat
      781 gaggttgccg caggtcgcat ccgcatacgt ctccaggctc acccaatccg tgaaaggagt
      841 ttcctccaga tcgtgctcgc gcgcgtcgat catcgcatcc aatagtgcgc gcgggatatc
      901 gtgcgaacga acagtctcgg ccaggacctg caccgcctcg tgacggcgcg cgtgcccggt
      961 atagatttcc tccaccgctt cgcgccacca ttgcaggcga atttgcccca tcaccggctg
     1021 gctgacactg tccgcggtct tcgcgacttc ataataaaag gcatagagcg cgacgagatg
     1081 cggccttggc tgcgcgtctg cgaacaatgc cgcgagccag cggtccgggt cctctctacg
     1141 gacgagcgtc tcacacgcag cttgatccag agagggcatc ggtttcacgc ttcgatgtgg
     1201 aatattccct catcctgagc ttggcgaagg atcgccgcaa aaataaaagg gccgcgcgtc
     1261 agcacggccc tttcgggtgc gggaaacgaa cgttcaagcc agatgcggag ccgccgccat
     1321 gcagaacaac atgggaatcg acagcatcgt attgacgcgc gaaaacatgc tcgccgagcg
     1381 tgccgccttg gccttttcgg ccgccggcag atcgggatag gcattggcga tgttgagcac
     1441 cttttgctgg tttggccaga tgatgaacca gacattgaac cacatgatgg tgccgagcca
     1501 catgccgatg ccgatcagga tcaccctgag atcgatgaaa ccctcaatgg catccagcgt
     1561 ataggcctgc agcagatagc cattcagggc cgcgagaatg atgccgaaga agatcgttcc
     1621 catcgcgccc caacggaacc agaacagggc ttccggcgcg atgaaacgcg acaccccgcc
     1681 cttcagctcg tcggggattt tcggcatcat cggtgtctgg acgaagttga aataccacag
     1741 aatgccaatc cacatgacgc cgcagatgat gtggaaccag cggatcagaa acggccagaa
     1801 cgtgaaattg ttgaattcgg tctgtgtgag ataaatcagg aaaacaatga aggcgagcac
     1861 gaagcctgca atcaccgtat tacgaagatt gctcagaatg gttgcgatca tagaacttgc
     1921 cccgaaagtt tcttgaaatg catatgtcaa ctataaggcg aatgacctga accgaatagc
     1981 gggtagatag aattcggcat cgctcggagg gttcctgtct tcacaggacc agcgcctttg
     2041 gccgcctctg gcgcagccag gtcacgacga tgagccgggt caccatgacc gcggtgaaga
     2101 acgatgtgat gacgcccaac cccaatgtca cggcgaagcc gcgcaccggt ccggtgccga
     2161 ccgcatacag aatcgccgcg gcaaggacgt gcgtcatgtt ggtgtcgatg atggtggcgg
     2221 tggcgcggcg gaagcccgca tcgatggcgg cgatcaccgt cttgccgctg tgcatctcct
     2281 cccggatgcg ttcgtagatc agcacgttgg catcgacggc catgcccatg gtgagcacca
     2341 tgccggcgat cccgggcagc gtcagtgtcg cctgcaaggc ggtgaggacc gcgatcagaa
     2401 gcagcatgtt gacgccgagc gcgaccgtcg cgaacagacc gaacaggcca taggtggcga
     2461 tcatgaagac gacgaccgcc aatagcccgc cgatggatgc gagcttgccg gcctgcaccg
     2521 aatccgcgcc aagttcggca ccgacggtgc gttcctcgat gacggaaaga gcggcgggga
     2581 gcgcgcccgc attcagcagg atggcgagat cgttcgcggt ttcgctcgtg aaattcccgg
     2641 aaatctgtcc cgaacctccg atgatcggct cctgaatatt gggcgcggag atcaccttgt
     2701 tgtcgagcac gatggcgaat ggcttgccaa cattggcctt ggtcacatcg ccgaactcgc
     2761 gagccccgcg cgtgtcgaag cggaaattca ccaccggcct gccgtcgcgg ggatcgaagc
     2821 ccgagccggc ttgttccagg cggtcgcccg agaccatgac ccggcgctgc accagatagg
     2881 gttgggtcgg ctggcccgcc cggtcggttt catagagcag ctcgtcatcg ggcggcacgc
     2941 ggcctttgcc ggccggcgcg ggcagcgcgt cctgcacatt gccgctgaca tcgacgaggc
     3001 ggaaggtcat cttggccgtg gtgctgagga cgcgcttcag ttcctcggga ttcttgaggc
     3061 cggggacctg caccaggatg cgatcgtcac cctgctgctg gatggtgggt tcgcgcgtgc
     3121 ccagcgcgtc gatacgccgg cgcaccactt cgatggattg cgtgacgatc tgcgtctgca
     3181 tctggcgcag ataggcgggc gagatgcgga aggtgaaatt gcccgtgccg tcatcggtca
     3241 tgtcgtattc ggccgcaccc aatccgagga aggtcccggg tggagacgcg atgtccttga
     3301 ggattttgcg ggcggcatcg agctgagtcg aatcggtgac gtgaagggtc acgctttcgc
     3361 gcgccgcatc caggccggta taattgatgc ggactttgcg caacgccgcg cggatgtcgc
     3421 cgcgaatggt ctcgaggcgt tccttgatca cgccgggcat atcgacctcg agcaggagat
     3481 aggagccccc ctgcagatcc aagcccagat tgatcgtgga actgggaacc cagccgggca
     3541 gcacaccgcg aacgcgctcg gaaagaaaat tcggcgccgc gaagatcacg cccaacagca
     3601 ggatcaggag aacggttatc gtctgccagc ggccgatgta gatcatggat tcctcctcgc
     3661 gggcctaaac gcgcccacgc cttcgattca gctttcgccc tttacgtcgc ccttggaccg
     3721 gacttcggtc agggtgctct tcaggatgcg aacctgcaca ttgtcggcga tctcgaccat
     3781 gatctcgcca tcgtccttca ccttggcgac ccggccgatc atgcccccgg aagtgaccac
     3841 ggtgtcgccg cgtttgacgc cttccaccaa ttgctggtgg gccttgaccc gctgctgctg
     3901 tggccggatc aataggaaat agaagatgcc gaacagcaga agcagcggca ccatagtaga
     3961 aacgagggga tcgttgaagt tcatggatat ctcttaaagg ggggctcttg ggtgcggccc
     4021 tgcggcgaat atagccttca gcccctcctt tgcaacggga gcgtggctgg gttaggcaat
     4081 gacaatgacc agggatttca ccaaaatcga ggacctgctc gcgcgcatcg ccgcggcgct
     4141 ggaacaacgc atgccccgcc cgccggagga catccagacc gtgcttgggg cctccgacgt
     4201 ctttgtctgg gaggcggaca ccgcccaact cgtggctgtg cggcaaacga cgggccttcc
     4261 gttggggctg ctcaagggca tcgacgcccc ccgcgacacg ctgttcgaga acacgcggcg
     4321 attcgcaggg ggactaccct ccaacaatgc ccttctttgg ggcgcgcgcg ggatgggcaa
     4381 aagctcgctc gtcaaagcgg tgcacgccga agtgaacgcc aaactggcgc cgtccaaccg
     4441 cctggtgctg atcgagatcc atcgcgagga catcgtgtcg ctgccccagc tcttacgtct
     4501 gctgcgcgga cagaaacggc agttcctgct gttctgcgac gatctctcct tcgaccgcga
     4561 cgacaccagc tacaaatcgc tcaaggccgc gctcgaaggt ggcgtcgaag gccgtccctc
     4621 caacgtgctt ttttacgcaa cgtccaaccg ccgccacatg atgccgcgcg atatgatgga
     4681 gaacgaacgc tccaccgcga tcaacccgtc cgaagccgtg gaggaaaagg tctctctgtc
     4741 ggaccggttc ggtctatggc tcgggtttca caattgcgcg caagacgaat atctcgccat
     4801 ggtcgaaagc tatgcggcgt atttccaatt gccgctcgca gccgatgcgc ttcgcgcgcg
     4861 cgcgctttcc tgggcggcgg ggcgcggcgc gcgttcggga cgcgtggcat ggcaattcat
     4921 ccaggatatc gccggagaat tgggcaaacc cttgcgctaa gatttggcgg cggtgcgcaa
     4981 acgtgccgcc gccggtttct tgcgcttgga cttgagcgcg ggcttcgcgc gcgcgagcga
     5041 tgtgagcgag gtttcgagca aaccacttag ccgctgctgc tcgcgcagct ccgtcaaatc
     5101 catgtgcagc ggcgtcacag aaatacaccc ctgcgcgatc gcgtgcagat cggtgccgcg
     5161 ttcgggatcg ccgaagatgc gcttgaagcc gacccagaaa taggtgcgcc cccgcgcgtc
     5221 gctgcgttcg tccacgcgca ggatcgactg atcgcgctgg ccttgcgcgg tgaccatgac
     5281 gcgcttgacc ttgcccggcg gcagcgcggg aaaattgacg ttcagcagca cgccttccgg
     5341 ccagccgatt tcgaccagcc gcctgaccag gggcgggccg tgcttctccg cacaggcgaa
     5401 gaggacatcc tcttcatagt cgccgccagc ggcctggctc atggcgatgg acggaatgcc
     5461 aagaaccgca ccttccatgg cggcggcgac ggtgcccgaa taggtgacgt cgtccgcgac
     5521 attgaagccg cgattgacgc cggaaatcac cagatcggga agatgatcct tcatgatgtg
     5581 gatgcccgcc atcatcacgc aatccgtcgg cgtgccggag agcgcgaagc gttgcggccc
     5641 ggcctgacgc agccgcaagg gaagcgtcag cgtgagggaa tgcgaggctc cggattgctc
     5701 ggtctccggc gccaccaccc agatgtcgct ggaaagcgag cgcgcgatct tctccgccac
     5761 cgccagaccc ggcgcatgga tgccgtcatc gttggaaacg aggatgcgaa gttgtttttt
     5821 acgggagcgc aagacgacac ggctcatcgt gcgatccggt cgacgccgcc catatagggt
     5881 ttcagcgcat cggggatcag gatcgaacca tctgcctgct gataattctc caggatcgcc
     5941 accagtgtgc gcccgaccgc aaggccagag ccgttgagag tatgcacgaa gcgggtgttc
     6001 ttttcgccgg gcggacgata acgcgcattc atgcggcgag cctggaaatc accgcaattg
     6061 gaacaggacg agatttcacg gaacgcgttc tggcccggca accagacctc gatgtcatag
     6121 gtcttacgcg cgccgaaacc catgtcgccg gtggacagca caatgatccg atgatgcagg
     6181 ttcaggcgct tcagcacttc ctgcgcgcat tgggtcatgc gctcgtgttc ggcggcggat
     6241 tgttcctgcg tggtgataga gacgagttcc accttcgaga attggtgcat gcggatcatg
     6301 ccgcgcgtgt cttttcccgc cgcaccggct tcggcgcgga agcacggcgt ataggcggtg
     6361 aagcgcaacg gaagctgtgc gccgtcgaga atttgctcgc gcaccagatt ggtcagcggc
     6421 acttcagcgg tgggaatgag ccagcttggt ggcagcatgc ctttggttgg accgaatctt
     6481 ggcgtgactt tgaattctcc ggcgttattg gtccacagat cagcactacc cacgggcttt
     6541 tcttcaaact ccacatagct acttgcgaag aacaggtccc tttcaaattt cggtagttgc
     6601 cctgtcccac gcattgcgtg gtcgcgcact agcagcggtg gtgaaatctc ggcatagccg
     6661 ccgatttcga gtttctcact tgtattgttt tcacgcacct gatccgtgtg cagatcgagc
     6721 atgaattgcg cgatggcgcg ttccaacctg gcgagctgat tcttcagcac aacgaagcgc
     6781 gcgccagaca tccgcgcggc ggcctcgaaa tccatctgac cgagaccctc gccgagatcg
     6841 aaatgctcct ttggcgtaaa agcgaaatcc ggcttcgccc cccagcggcg aatttcctta
     6901 tttgcgcttt catcgccgac cggcacatcg tccgcgggaa tattcgcaat cccggcgagt
     6961 agatcgtcca attccttttg gaatcgccgt tctttttctt cgccgtcctg aattgtttcc
     7021 ttaatgaccg caacttcgcc catcagggcg gtagcctgag cctcgtcctt cttggccttc
     7081 gcctgtccga tcagcttgga ggcttcgttg cgccgcgcct gagactgctg aaggatggtc
     7141 tttatattgc gcaaaatttc atcgcgcttc agaatctccg ggctgatggg cgcgaacccg
     7201 cgcttcttca gtccttcgtc gaaggccgcc ggatcgtcgc ggatcgcttt gatgtcgtgc
     7261 atgggaaaat tctccaaccg ggaggcgttc tggcatgggc cgcgccggtg cgcaaaggta
     7321 aactatgtcc gcccatcagg aggaaccgat gtccgaagac gaacacgcga ttcgcgctct
     7381 catcgccgat tggatcgccg ccagcaaagc gggcgatctc gccaccgtgc tggggttgat
     7441 gtccgacgac gtggtgttca tggtcccggg ccgcagcttt ggaaaacagg agttcgctgc
     7501 cgcgtccgcc ggcatgaagg gcatggattt catcggtgag agcgaggtgt tggaaatcga
     7561 agtcctgggg acacgcgcct ggtgccgaac gcatctcacc gtgactgtga cgccgccgaa
     7621 tgcagcgccc gtgcgacgat cgggctacac gttgtcgatc cttgccaaga acgcaggagg
     7681 aaaatgggtc atcacgcggg acgcgaatct gctggcggcc gggtgattct tcaggtcggc
     7741 tggatgtcgc cgggtcccgc cgccgccgct tcggccttgg cgcgctgacg ctcgatcagt
     7801 ttcacgcaga ggatcgaaac ctcgtagagc ccgatcatcg gaatggacag cgaaagttgg
     7861 ctgaagacgt cgggcggtgt cactaccgcg gcaagcgcga caatgccgac gatggcatag
     7921 cgccgcgcgc tcgagagcgc cttgctgctg acgatgccga cgcggcccaa aagcaccagc
     7981 agcaccggaa gctcgaaaca aagtccgaac gcgaagatca gcgtcatcac gaaggtgaga
     8041 tattcgccca ccctgggcgc gagctggacg gcgatctggc ccggaccggc cggaatctcc
     8101 tcaccgacga agaactggat cacgcgcggc agcatcagat aatagacaaa ggccgcgccc
     8161 gtgacgaaca tcaccggggt cgccagcagg aacggccaga aggctttgcg ctcgtgcttg
     8221 taaaggccgg gtgcgatgaa cagccaaagc tgcagcgcca tatagggaaa ggccaggcac
     8281 aacgcgccga acataccgat ccgcacataa gtgaagaact gttcgtatag cgcggtgaag
     8341 atcatctggc gattcgcctg gcccgccagc gcatcggaaa tcggccgcgt caggaacgcg
     8401 aaaatcggct tcgagaaggc gaagcagaag ataaagcaga ccaggaacgc gatcaccgaa
     8461 cgcacgatac ggttgcgcaa ctcgatgaga tgttcgagca gcggcgcttt gctcgattcg
     8521 agctcggctt cttcgtcaga gctgggtgag gccatgaatc gaccctacac cgtcatggcc
     8581 agcaggacgc catccgtgcg cctagcgccg cgttgcgcgg ctctgcggag gtgacacagc
     8641 gaacccatca accgtttgtc ccggtgacgg gctgcgtcgc tttcgcggct tcttccgccg
     8701 cggcctcggc accggtctca accggctggg gcgcactcgc cggaatgggt ccggccggaa
     8761 ccggtgcggc cgccgggggt gtatcgaata tcgggggcgc cgcgcccacc tcttcgatgg
     8821 tcgattggat gccgagcggg tccttgggga attcgattcc cagggccgcg taatccttgt
     8881 cggtcatggg ttgggcagct tcaacggcct gggccttcat cttgttgact tcgttgcgca
     8941 gttcgtcgag ctcggcctgg cgcgccatgt cgtcgaagct gcggcggaac tgatcggcca
     9001 tggcgcgtgc gcgtccggcc cattggccca ccttccgcat cagcttgggc aggtctttgg
     9061 gaccgaccac caccagcgca acgatcagaa tgacgagata ctcgctgccg tcgatgccga
     9121 acatctgctt ctctcacgag cacccccact gccatgattc cggagtgggg tccgcaatgg
     9181 gccctatgac cgctgggata gtccgtcacg acccaggacg ggatcgctca gccctgcttg
     9241 acttcgtcgg ccttcttggc ttcgacctta tccgccggcg gcgagggatc gtttggatcc
     9301 ttcagccctt ccttgaagtt ccggatgccg tgggcgaaat cgcccatcaa tcccgaaatc
     9361 ttgcccttgc ctccgaacag gagcgcaacg accgcgatca ggatcagcca atgccaaatg
     9421 ctaaaactgc ccataggctc atactcccgt gattgaggca tgatagcgcg ctatgcgaag
     9481 gctttgcgcg gttttctttg cccaacctaa ttttccgtgc cctatctagc ctgatccttc
     9541 acttgcgaca aatctcattg cggcaaggaa agacaaaagc ctcctgtggt tccgcggcga
     9601 ggcagacaac cgatccgggc accagacccg tacccgccgg tgccgctgcc cttaccgaaa
     9661 tggggacttc caccccttcg ggcaaggcat tcgtgaaaaa cgccgcttcg atttcgagct
     9721 ggccggcata gggtctgacc gccagcacgc gagcctggac acccgtgtcc gcgacccgca
     9781 ccgaggccgg ccggtacaat atctccgccc attccccctc ggctaccgat ttcgcagacg
     9841 tttgaccgaa aggtgatgcg gcgcaccctt ttgtgacgcg ggcgtgaaac acattggcgc
     9901 ccccgaacag agccgccgcc tgggggtccg cggggtgccg atagatttcg gccggggtgc
     9961 cggtctgaac gatgcacccg gcgcgcatca gcgcgacccg gtcgcccacc cgcatcgcct
    10021 cgtccgggtc gtgggtcacc atgacgaccg ccgcaccctc ctcccggagc cgcttgagcg
    10081 tggtgcagcg cacatcgtcg cgcaaagccg agtcgaggcc ggaaaaaggc tcgtccatca
    10141 gcaccacgtc cggcttgggc gcgagcatgc gggcaagcgc cacgcgctgc tgctcgccac
    10201 ccgaaagcgt gtggggaaac gcgtccctga ggccggtaag cccaacacgc gcaagctcat
    10261 cgtccacgcg ggcctcgcgc tccgcccgcg cgagcttggc cagaccgaag gcgacattgc
    10321 gcttcgccgt cagatgcggg aacagggcgt aatcctggaa catcaaaccc acccggcggg
    10381 tctcgggcgg ttcgtgacgg ccctgcccct ccaccaaacg cccgcccacg aacacgagcc
    10441 ccgaatcggc acgctcaagc ccggcagcga tgcgcagact ggtggatttc ccgcatcccg
    10501 aaggaccgag caaacacagg acttcgcccc gcttcacgtc gagcgtcacc ccgtccaccg
    10561 cgatgcgggt gccaaagcgc cgcgtcacat tgcggagcga aagcccgtcg gcgggggcgg
    10621 tgcatgtttc gtcagagatc tgcgaagccg cgttcagaag gcattccttt gcgaatgcgt
    10681 atcgcatgct aattaggcgc tattgggccg gcaccgcaag caatcctccg cgcttggagg
    10741 aaccttgtga caagttttgg cggccgccag attgacaaat aaggaacatc gcccctggcg
    10801 tatggttaac atgcacgaga ttcgcgtctc gcgcacccaa ccaactcaag ccccgcttct
    10861 cagtggggct ttttcttatt agaggggctt tttcttatcc ggattttttc tgatccggtg
    10921 ggctgaaaac catggtcaaa ctagagcggt ttgcggtttg cgcccgaaaa catcggccaa
    10981 atcggcaagg cgcttgtgcg ccgtttcgcc gagcagcgct tttttcgcca agggaagctc
    11041 cagcgccagc gtatctttgg tcagcatcaa cttggcgtgc ggcaactcgc cttcgaccgc
    11101 ccccgaaagt ccgaacgcca ggcgcgccgc gagtccgatc tgctgcgcct tcaccgcgcc
    11161 gtccgcgccc agaagactcg ccacacgcgt ctcttccggg aaatcatcgt cgccggcata
    11221 gcggtaatag gcggcacttg ccatcagcgc gcgttcatga tgatcggccc cgccataggc
    11281 accccgcagg acctggacaa aggtccccat ggcgcgatcg tcgggatggc gccgccagcc
    11341 aatatccgag aacagacacg ccgcctcgcg gatgcggcgc tcggctgccc cctccttggg
    11401 aaacagcggg gtcatccagc ggaacagttc cggcgcatga ccgggaaggc gcgattcgcg
    11461 ctcgttcaga tcgcgggcaa acgcgatcag cgggtccttg gcggcctctt cgggtttgag
    11521 ctttcgctgc aggacgccct cgcgcaagcc gaaggcggaa acgacgacct ccttgaggcc
    11581 aaggccctgg atcatctcct ccatcaccag cgcgccatag ggcagcgcct cggcacggcg
    11641 tttgggcacc gagcgcatct tctccagcga tttgcgcgaa agccccgaca ccacgcggca
    11701 cagcttcacc gcgcgaccgg cgggaatgac gtaatgatgc agcacccgga gcggatagtc
    11761 gcgctcatcc atgtcgacat gagcaagggc gcgccagatg ccccccaccg cataaagcgc
    11821 ccggcccttc agagatttgg cgaaatcgag tttgccgagg cctttctcga cgacgttgcg
    11881 cgccttgttg agatttgcgc ccgccacatc catcaagcgc aagggtccaa agggaagtgt
    11941 ggcggcggcg ccagtcttgc cgtccttcac cgtcaccatg tccaggcttc ccccgcccag
    12001 atcggcaacc aggccgtcgg cgtcgggaag tcccgcgagc gttccttccg cggcgatgcg
    12061 cgcctcttcc tcgccggaaa gaatccggat cggctgaccg agcgtctttt cggcgcggcg
    12121 gatgaaatcg cggccattcg tggcgtcgcg cgcggccgcc gtcgccaccg cgccgacatc
    12181 gcgcacattg tgtccgtcac agagttcgcg aaagcgcgcc agcgtctcga tcgcgaaaat
    12241 catgccctcc gcatcgagat tgccggtgga gaccatgttg cggccgatcg cgcagatcgc
    12301 cttttcattg tggagcgcgg ccgcatgccg ccacggccct tcatagatga ccagacgcac
    12361 cgagttcgag ccgatatcga caatggcgat cggctgatag cggcctatcc tgcggctgct
    12421 cgccttgaac tgaacatcgg ttaggctcaa tcggactcca tgaatggtca ggcgccgcga
    12481 atcgcaccgg cgacgttggg aggagtatcc accttcaagg cccgcccgcg acccgaaagc
    12541 gacggattgg tcatgaaata ggtgtgtgcc gaaaacgcat cgggccgatc gaatgccggg
    12601 ttgcgggcat aggagccatc ggcccgcagg acccagctct gcgcctgatc cttgagattg
    12661 gcgaccatga tctgggacag cacctgctga tgcacggtgg gattgtcgat cgggaccatc
    12721 gcctccacac ggcggtcgag gttgcgcgtc atccagtccg ccgagctgat atagaccttg
    12781 gctttcggtg acggcagccc ataacccgcg ccgaaacaga cgatgcggga atgttcgagg
    12841 aagcggccga caatgctctt tacacggatg ttgtcggaaa gtccggcgac acccggtctg
    12901 agacagcaga tgccgcgtac caccagatcg atctgcacgc cggcctggct cgcgcggtag
    12961 agcgcgtcga tgacgcgcgc atccaccagc gcgttgagct tggcccagat cgcggccgga
    13021 cgaccggcgc gggcgtgttc gatctccgct tcgatgtaat ccatcaaccg gttcaccatg
    13081 gtgagcggcg agatcgcgat cttttcgagg tggctgggct cggcgtagcc ggtgacgtag
    13141 ttgaagaact gcgccgcatc gcggcccaag gccggatcgg ccgagaacag cgacagatcg
    13201 gtatagatct tggccgtcag cggatggtaa ttgccggtgc cgaaatggac gtagctgcga
    13261 agctttccgc tttcgcgccg caccaccagg ctcaccttgg cgtgggtctt aagctcgatg
    13321 aacccataga cgacctgggc gcccgcgcgt tcgaggtcgc gcgcccattt gatgtttgcc
    13381 gcttcgtcga agcgcgcctt cagctccacc agtgccgtca ccgacttgcc ggcctcggcc
    13441 gcttcgatca gcgcgcgcac gatggggctg tccttgctcg tgcggtagag cgtctgcttg
    13501 atcgcgacga cgtctggatc ctgtgcggcc tcgcgcagga actgcaccac cacatcgaaa
    13561 ctttcaaaag ggtgatggac gatgaggtcc tttgccttga cggccgcgaa gcaatcgccg
    13621 ccatgatcgc ggatgcgttc gggaaagcgc gacgtgaagg gttcgaactg caggtcgggg
    13681 cgctcatcga ggatgagctg cttggtttcg tccaggccca gaagcccatc gaccacgaag
    13741 ggttcacgct gccccatata cagatgatcg gcgatgaagc gcatcaggtc gggcggcatg
    13801 gaggcgctgc atttcaggcg tatcacagag ccgcggcggc ggcgcttcaa cagcgtttca
    13861 tagaggcgca ccaggtcttc ggcttcttcc tccacttcga tgtcgctgtc gcgcaggatg
    13921 cgaaacacgc cagcggccgt gacggcatgg gcgggaaaga gacggtcgag ataaagccca
    13981 atgagatttt cgagggtgat gaaacgcgct ttgccctccc ggtcgggcag gcgcacgaaa
    14041 cgctgcaatt gcgagggaat gggcagaagc gcggtgagcg ccttgccatc catgggccgc
    14101 gtcagccgca tcgccatcac gaagccgaga ttgggaatga acgggaaggg atgagcgggg
    14161 tcgatggcga gcggcgtcag gatcgggaag atctgctggc tgaattcctg atccatccaa
    14221 gcgcggtctt ccaccgacaa ctgatccggc tcgatcacgg caatgccgga attgacgagc
    14281 gccacgcgca actcgcgcca ggtcttctgc tgatcgagga tcagctcggc cgccatctga
    14341 tcgacgcgcg aaagctgctg cgccggcgtc aggccgtcgt ccgaaaggct cgccacgccg
    14401 gcattgacca tgcctttcag gccggccacg cgcaccatgt agaattcatc gaggttcgat
    14461 gccgaaatgg aaagaaagcg aagccgctcc agcagcgggt ggcgaagatt ctgcgcctcc
    14521 tccagcacgc gccgattgaa ggcgagccag gacagttcgc gattgatgaa gcgcccggga
    14581 gactgcaacg agagcccggg gataagcgtt gccgccgccc ccgaagccgg catgtccggc
    14641 accgcttccg ggcctgtggc ggtgacaccg cttgccgcta tttctctgag tgttgccttg
    14701 ttcatcggag atttccgcct tgccgttgag tgtaatgatt aattccggcc ctgtcatggc
    14761 gggcacgcag gcctagaatg gcgccttttc cgtgaaacaa atgcgaatgt ggacgttcag
    14821 tcccgcgacc cggacggggt ttcggactca aggagttcga gggccagccg ttcggtgacg
    14881 gcacgtttct cgccgagggc cttctcgtcc agccgcgcga taaaggcggc aatcgcggct
    14941 ggcgtgcgct caagctggat cacgatgcgt ctgatgacgg cttcgggcac ctcaagctgg
    15001 cgatccgcga aatgcttggc gatgagcccc gccagcagca catcgtcggg cgcccacatc
    15061 gcaaaaccga tgagcgccga aaatcgcgag gccagatcgc cgatgggact ttgccacgca
    15121 gccggacggg tacgcgcggt cagaagcagc caccctttcg ggcgctcgaa cagagccagc
    15181 agagcacgat cgcgggtttc gccggggggg gcacggtcaa aatcttcgat caccagagcc
    15241 gtgccactat cctgcgcgtc caaattttcc ggcctcagcg cataggcgtc gatgatttgc
    15301 gcgccggcgg tctcgcgcca tatcgcggcg agatgactct tgccgcagcc cggcggtcca
    15361 tagagagccg cagcgtacga cggccaatcg ggccagcgcc ggatgaattg gaacgccggt
    15421 tcgttgcacg gggcgacgat aaaatcatcg cgcccatggg cggacttcac gccgaacggc
    15481 agggcgagtt ggccaaacat caaagcatgg gcatcaatgc atgggtcagg cccgccggcc
    15541 ttcctgtacg ttcacggtgc gcatggcctt gagccagacg gccaaatacg cgaaccccga
    15601 gacccaggcg aaggccgcga ccagataggc gtcggcgggt gcgcttgcgt ccaccggcag
    15661 atcgaaagca agcgccgcca aatgccaccc gatgtagatc acttgcagcg ccgtacagac
    15721 cttgccgcgg aaaaggggct ggacggcgat gggcgcccgc aaggcgagcg cccaagcgag
    15781 cccaagcagg atcagaagat cgcgggcaat caccgtgacg gtgagccatg ccgggacgtc
    15841 atcgagaatg gcaagcgtca cgaaggctgc gatcatcagc gccttgtcgg cggcgggatc
    15901 gagcaaacgc ccaagccggc tcgccagatt gaaacgcttg gcgaggaatc catcggcagc
    15961 gtccgacaat ccggcgaatg cgaagagact gaatgccgcg ctgaaatgcc ccgcgaccag
    16021 cagaacagcc gtcgtcggcg ccgcggcgag cctgagcgtc gtgatcatgt tcggcaggtg
    16081 gcgcagcatg aacatcatgg ggcgttggcg gcggatcgac cagcaagctg cagggtgaac
    16141 tggccgcccg cattggcgag caaaagattc tgctgagcca aggcatcgcg caattgttcg
    16201 ggccgtccga aatagaccag ttgaatctcg gcttcgtgca gcgcaagccc ggtcacatcc
    16261 atgtcggcca cggctttcac ggcgccaagc tgctttctga tcttggccca atcgtccagc
    16321 gtctcgaact ggacatcggc ggtgagttgg ccgcgcgtgg cgaaattcac ggcactgcgg
    16381 gtcttccagg tctcgtaggc tctttcggcc accacctcgg catccgcggc gaaggtggaa
    16441 ttggcgtagg caaaggaggc cgcgatgcgc gccgtcgccg aaatttcaac catctgcacc
    16501 gtcttggcgt cttcgctggc gatggcgatg atcgcctccg tggcgttgta gcgccgcgcc
    16561 agcggcgcta ggcttgccca atcggcctga gcgagatcgg gccgggacag gatttccatg
    16621 tcctgagcgt ctgccgccgg cagcacaaaa ggcaccagac cctgctgcaa ggcgggatcc
    16681 ttccaggcca tggcccaagc gctcatcgga tcgacgccgg cggtccccgc gacaatcggg
    16741 ataaccagcg acgggcgggc tcgcgtctcg gtataggcga tccccgattg tctcaggaga
    16801 gcgcgaaccg ccgcggcgtt aaaacgatag gtcacctcgg ccagataacg cgtggtgctg
    16861 cgccgctcgt tggagacttc gaagctgcgc accatgcgca gcatcgcgtt gtcatccagc
    16921 ttcggctgct tgccccaatt ggcggttgcg gtcacacgcc gataaatctt ggtccacgcc
    16981 aacgggcggc cctccgccat ggcgagatcg cgggcggccg tggccgattc ggcggtcgcg
    17041 tccaccttga tgccggcgac ggtgaagatg tcggggacag gcgcgggtgc tgcctgaacc
    17101 gcgcctgccc gcaggatgaa acccgcggcg agaacgagaa gggaaaggcg aagcatggga
    17161 ttcctctata gcctgactat agggataggt atggacagaa aaaaggcatc gtcgcgaagg
    17221 aaacgccgaa tccgccggtc gtctccggcc agcctcttaa tttattcgcc gtatgcgcgt
    17281 ccaattatgc ccgaatcagg cctttgggag cggcagcggt cgcgctttcg ggaaagattt
    17341 tttcttcaag tgcctgtttt gacacgccga gatgttcggc aagagcggcc ttgaagagtg
    17401 cccgtgtgtc ggtctgggcc ggcagatcgc gtccgtccct gagcacagac ggtttcaggc
    17461 cccgccattc ggcgtggata ttcccgcccg cgaccgcgcc gccgaacatg aaacctatgc
    17521 cggcggtgcc atgatcggtg ccgcccgtgc cgttcacttt gacggtacgg ccgaactcgc
    17581 tcgccgccac gatggcggtc ttctgccagg caggaccgag cgatgccttg agcgccgcca
    17641 agccttcatc gaggagctgg agccgacggg ccaattggcc ctcgcccgca ccctgattgg
    17701 aatgggtgtc ccagccggtg atgtcgaaga cggcaacacg cggaccgcct tcggcggcga
    17761 gaatccggcc ggcgccttcc ataagaggcg cgatatcgcc ggcggcccct g
//
LOCUS       DASYEG010000248         2604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1830350, whole genome shotgun sequence.
ACCESSION   DASYEG010000248 DASYEG010000000
VERSION     DASYEG010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1830350"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2604)
                     /gene="metH"
                     /locus_tag="VGM72_10810"
     CDS             complement(<1..>2604)
                     /gene="metH"
                     /locus_tag="VGM72_10810"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEY4265800.1"
                     /translation="MIQNYRLSEKDFRGARFADSPRDLKGNNDLLVLTQPDVIREIGR
                     AYLHAGADIIESNSFNSTSISQADYGLESLVPELNETAARIAREVCDEVATPERPRFV
                     AGVLGPTNRTASLSPDVNDPGYRNTSFDALAETYADATHALIRGGADLIMIETVFDTL
                     NAKAAIYAIEEVFAQTGERLPVWISGTITDLSGRTLTGQTVEAFWHSLRHAGPFAVGL
                     NCALGPKELRPHVADLSEIADTLTSAHPNAGLPNAFGGYDETPHSMAAHIGEFAREGL
                     LNIVGGCCGTTPEHIRAFVETVAGVPPRKIPEKPRQLRLSGLEAFTLTPETNFVNVGE
                     RTNITGSAKFRKLITAGDYEAALDIARGQVESGAQIIDVNMDEGLIDSEAAMARFLNL
                     VAGEPDISRVPVMIDSSKWSVIEAGLKCVQGKPVVNSISLKEGEAAFVAAAEKVRRYG
                     AAVVVMAFDEKGQADTAERKFEICRRAYGILVDRVGFPPEDIIFDPNIFAVATGIEEH
                     NDYGRAYVDATTRIRQGLPFSHVSGGVSNFSFSFRGNERMREAMHSIFLYHSIRAGMD
                     MGIVNAGNLPVYEDIPAELRDAIEDVLFNRRADATERLLELAQKYRKSGEEADPEVVL
                     AWRSLPVEERLTHALVHGIADFVLEDVEAARLRAKKPLDVIEGPLMSGMNVVGDLFGS
                     GKMFLPQVVKSARVMKKAVAHLIPFMEAEKEGAQRESAGRIVMATVKGDVHDIGKNIV
                     GVVLQCNGYEVEDLGVMVPSARILEIAREKKADVIGLSGLITPSLDEMCHVAAEMERE
                     GFDIPLLIGGATTSRVHTAVKIHPNYARGQTVYVTDASRAVGVVASLISKETRASVIT
                     DVRSEYARIIAS"
BASE COUNT          495 a          822 c          798 g          489 t
ORIGIN      
        1 ggaggcaatg atgcgcgcat attcactgcg cacatccgta ataaccgacg cgcgcgtctc
       61 cttggagatc agactcgcca ccacgccgac ggcacgggaa gcatcggtca catacaccgt
      121 ctgcccgcgc gcataattgg ggtggatttt taccgcggtg tggacacggc ttgtggtcgc
      181 tccgccgatc aggagcggaa tatcgaaacc ttcgcgttcc atctcggcgg cgacgtggca
      241 catctcgtcc agcgacggcg tgatcagacc ggatagtccg atgacatcgg cctttttttc
      301 gcgcgcgatt tccaggatgc gtgcggacgg caccatcacg cccaaatctt caacctcgta
      361 gccgttacac tgaagcacca cgccgacgat gttcttgccg atgtcgtgga cgtcgccctt
      421 gaccgtcgcc atgacgatgc gcccggcgga ttcgcgctgc gcgccttctt tttccgcctc
      481 catgaaagga atgagatgcg ccaccgcctt cttcatcacg cgcgcggact tcaccacctg
      541 gggaagaaac atcttgccgc tgccaaacag atcgccgacg acattcatgc cggacatcaa
      601 tgggccttcg atcacatcca gcggcttctt ggcacgcagc cgcgcagcct ccacatcctc
      661 cagcacgaaa tcggcgatgc cgtggacgag cgcgtgcgtc aggcgctcct cgacgggaag
      721 cgagcgccag gccagcacca cctcgggatc ggcctcttcg ccggatttgc gatatttctg
      781 cgccagttcc agcaaacgct cggtcgcgtc cgcgcggcga ttgaacagca catcctcgat
      841 agcgtcgcgc aattcggcgg gaatatcttc gtagaccgga agattgccgg cattgacgat
      901 tcccatatcc atgccggcgc gaatcgaatg gtagaggaag atcgaatgca tcgcctcgcg
      961 catgcgctcg ttgccgcgga acgagaagga gaaattcgaa acgccccccg acacatgaga
     1021 gaacggcaag ccctggcgga tgcgcgtggt ggcatcgaca taggcgcggc cataatcatt
     1081 gtgctcctcg atgccggtcg caaccgcgaa aatattggga tcgaagatga tgtcctcagg
     1141 gggaaagccg acgcgatcga ccaatatgcc gtaggcgcgc cggcaaatct cgaacttgcg
     1201 ctcggcggta tcggcctgcc ccttctcgtc gaaggccatc acgaccacgg ccgcgccata
     1261 gcggcgcact ttttcggcgg ctgccacgaa ggccgcctca ccctctttca ggctgatcga
     1321 attgacgacg ggcttgccct gcacgcattt cagtcccgcc tcgatcacgc tccatttgga
     1381 ggaatcgatc atcaccggca cacgtgagat atcgggctcg cccgcgacca gattgaggaa
     1441 ccgcgccatc gccgcttcgg aatcgatcag cccctcgtcc atattgacgt cgatgatctg
     1501 cgcgccgctt tccacctggc cgcgcgcgat gtcgagggcg gcttcgtaat cgcccgccgt
     1561 gatcaatttg cggaatttgg cggaacccgt gatgttagtg cgctcgccga cattgacgaa
     1621 gttcgtctcc ggggtgagcg tgaacgcctc cagtccggaa agacggagct ggcgcggctt
     1681 ctcggggatc ttgcgcgggg ggacgcccgc gaccgtttcg acaaaagcgc ggatatgctc
     1741 gggcgtggtg ccgcagcagc cgccgacgat gttcaacagc ccctcgcgcg cgaactcgcc
     1801 gatatgggcg gccatggagt ggggcgtctc gtcatagccg ccgaatgcat tgggaagccc
     1861 cgcattggga tgggcgctcg tcagcgtgtc ggcgatttcg gaaagatcgg ccacatgggg
     1921 gcgcagttcc ttggggccga gcgcgcaatt gagcccgacc gcgaacggcc ccgcatggcg
     1981 cagcgaatgc cagaaggctt ccaccgtttg ccccgtcagc gtgcgcccgg aaagatcggt
     2041 gatggtgccg gaaatccaga cgggaagccg ctcgccggtt tgggcaaaca cctcctcgat
     2101 ggcgtagatc gcggccttcg cattcagcgt atcgaagacc gtctcgatca tgatgaggtc
     2161 ggcgccgcca cggatcagcg catgcgtcgc atcggcatag gtctcagcca gcgcatcgaa
     2221 gctggtgttg cgataccccg gatcgttcac atcgggcgac agcgatgcgg tgcggttggt
     2281 ggggccaagc acaccggcga cgaagcgtgg ccgctccggg gttgcaactt cgtcgcaaac
     2341 ctcgcgcgcg atgcgcgccg cggtctcgtt cagctccggc accagcgatt ccagcccgta
     2401 atcagcctgg ctgatggagg tggaattgaa gctgttgctt tcgatgatat ccgcgccggc
     2461 gtgcagatag gcgcgcccga tctcgcggat cacgtcgggc tgggtcagga ccagaagatc
     2521 gttgttgcct ttgaggtcgc gcggcgaatc ggcgaaccga gccccccgga aatctttttc
     2581 cgacaggcgg taattctgga tcat
//
LOCUS       DASYEG010000249         5521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_962934, whole genome shotgun sequence.
ACCESSION   DASYEG010000249 DASYEG010000000
VERSION     DASYEG010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962934"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VGM72_10815"
     CDS             complement(<1..908)
                     /locus_tag="VGM72_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEY4265801.1"
                     /translation="MASLDSFKARRKLTVGNKTYVYFSLKAAEKNGLAGISRLPNSLK
                     ILLENLLRHEDGRTVTVADIRAVADWLKTRSSDREIAYRPARVLMQDFTGVPAVVDLA
                     AMRDAMAALKGDPEKINPLTPVDLIIDHSVQVDFFNGKEAFKKNVATEYQRNGERYSF
                     LRWGGQGFNRFRVVPPGTGICHQVNLEYLAQTVWTDKVKEGKTSVEYAYPDTLVGTDS
                     HTTMINGLSVLGWGVGGIEAEAAMLGQPISMLIPEVIGMRLTGKLRAGCTATDLVLTV
                     TQMLRKKGVVGKFVEFFGEGLGDMPL"
     gene            1167..1913
                     /locus_tag="VGM72_10820"
     CDS             1167..1913
                     /locus_tag="VGM72_10820"
                     /inference="COORDINATES: protein motif:HMM:NF027577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4908 domain-containing protein"
                     /protein_id="HEY4265802.1"
                     /translation="MRLQLSLGLSLALVLGAGLGASAQELPAAHLVLTPSMPLPRDGT
                     YIANDKLAFVLDHYGNQVRLRFLDNDEVFYLSSEPASLGGRVLKYDTGDTALQVTGWG
                     GLTLYTMQAKGGLPAESNDADQSVEPAPVGPKEVKAFAAKLAQDLSASEDFAVGFAAD
                     WDNLAKIDELRALGCDAMRNVTYALKELADSGRRALISDRIHIIRVIQGPKPAVTMQK
                     GILTVTVAPQLGLSARPSSLAIVQVLRASF"
     gene            complement(1963..2562)
                     /locus_tag="VGM72_10825"
     CDS             complement(1963..2562)
                     /locus_tag="VGM72_10825"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY4265803.1"
                     /translation="MKERRHVHAVVFDLEFTAWEGSLESRWTRPGELSEVVQIGATKL
                     DAASLTEIDCFDMLVRPRVNPVLSDYLTALTGITNAAMDHRGVDFITAYRAFLDFVGG
                     SETFAHGRDDLILTGNLKLYGWAHALPLPNYTNAIPWFTAQGIDLKGKRACDVAELAG
                     AVFHGRRHDALADARGVAAGFAVLIAKGAPNPFLGERSQ"
     gene            2720..>5521
                     /locus_tag="VGM72_10830"
     CDS             2720..>5521
                     /locus_tag="VGM72_10830"
                     /inference="COORDINATES: protein motif:HMM:NF016944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEY4265804.1"
                     /translation="MARTSSVVQTMHGAAAEEARFEAADAGAHLATASHLIGTGEPEL
                     AAFFTAFTRYASPEDLIHYTGAELASLVSGLHARLARRLAGTSLVQIFAPSSEDAGFA
                     RNAETVVLAVNDDIPFLYDSCTAEIRSRGYRIAAAFHPVVALARDASGTLSPKGSPLT
                     ESVIVLALEGVLDAEEAADLRDGLIEVFAGTRAVVRDWKPMLERIAETAASLNRNPPQ
                     VSEAELEENIAFLDWMADEHFTFLGCRDYVFSGEGEGRLDPVYESGLGLLADPEMRVV
                     RRGQDRSRLTPAVREFLKKPTPLIITKSALRSPVHRRVHMDYVGVKTFDPKGRLTGER
                     RFIGLFTSNAYSQLPVAIPLLRRKTAAVRAGSGLPPSSHDGKALQHILDTFPRDELFQ
                     ISEDELLATALGILNLGQRPKVRIFLRFDRFDRYVSALVFIPRERYSGAVRDTVHAIL
                     ARAFNGRQSAATPMLDDEALARVHYIVGRNEGPRPEADVKELESEIRSAIRTWDDGFA
                     DAARFAHGEITGGVSLRYAAAFPAGYRDAFTPAEAVEDIGHIETVLRNEGAAGPLAAH
                     FYSRNGDVPDALHLKLFVHGLFVPLSECLPVFENLGLRVIAEDAFALAPLAEDGTGQS
                     VALQNFLMARSDGKPVEIERLRPLLEDAFHAVWRSDAESDGFNRLVTAAGLDWRDVVI
                     LRAIAKFLRQAGLALSQTYVESALAKNPALAVFLVQLFRTRHDPEAFETAAPRNRAAD
                     EIRKTIQTALDDVPSADDDRIIRAMLSVIDAMLRTSAFQRDASGNPRSYLAFKLESKR
                     LDMLPAPKPLYEIFVYSPEVEGVHLRFGKIARGGIRWSDRAEDFRTEILSLAKAQQVK
                     NSVIVPVGAKGGFYPKTIPAGAAREVVQATGIAAYKTFIGALLDLTDNIGPGGSVISP
                     AHTLRYDGDDPYFVVAADKG"
BASE COUNT          999 a         1802 c         1656 g         1064 t
ORIGIN      
        1 tccagcggca tgtcgccaag gccctcacca aagaattcga cgaattttcc gaccacgccc
       61 tttttgcgca gcatctgggt gacggtcagc acgagatcgg tcgccgtgca accggccctg
      121 agcttgccgg tcagacgcat gccgatgact tcggggatca gcatggagat cggctggccg
      181 agcatggccg cttccgcctc gatgccgccc acaccccagc cgagcaccga aaggccgttg
      241 atcatcgtgg tatgggaatc cgttccgacc agcgtgtccg gataggcgta ttcgacgctg
      301 gtctttccct ctttgacctt atcggtccac accgtctggg ccagatattc gagattgacc
      361 tgatggcaga tgccggtgcc ggggggcacc acgcggaagc ggttgaagcc ctgcccaccc
      421 cagcgcagga acgaatagcg ctcgccattg cgctgatact cggtcgccac attcttcttg
      481 aaggcctcct tgccgttgaa gaaatcgacc tgcaccgaat gatcgatgat gagatcgacc
      541 ggagtgaggg ggttgatctt ctccggatcg cccttcagcg ccgccatcgc atcgcgcatg
      601 gccgccagat ccacaacggc cgggacgccg gtgaaatcct gcatcagcac acgcgccggc
      661 cggtaggcga tctcgcggtc cgaactacgg gtcttcagcc aatcggccac cgcgcggatg
      721 tcggccacgg tgacggtccg gccatcttca tggcgcagca gattttcgag caatatcttg
      781 agcgaattgg gcaaccgaga aatgccggca aggccattct tctccgcggc tttgaggctg
      841 aaatagacat aggtcttatt gccgacggta agcttccgcc gcgctttgaa actgtcgagg
      901 ctggccacga atgcactccc gtttgtccga atatgctgaa aacgggcgcg aacttagtcc
      961 taaagtcacc gtcaaggcta cgagataaac tttagcgccc gttttaccgt ctggggggca
     1021 ttaaggaggc cgcacgccgg aacgtgaacg cgcgtccata ttgttgccac cttcggagcg
     1081 ttcttgggtg tgggggttgg caacatcagg cgggggaacc ggccggtggg ccatgtcaga
     1141 atgtgtattt tcgcgagatt gatggcgtga ggctgcaact ttcccttggc ctgtcattgg
     1201 cgcttgtcct gggcgcgggc cttggcgcga gcgcccagga attgccggcg gctcatctgg
     1261 tcctcacccc ttcgatgccg ctcccccgcg acggcaccta tatcgccaac gacaaattgg
     1321 ctttcgtgct ggaccattat ggcaaccagg tccgcctgcg cttcctcgac aatgacgaag
     1381 tgttctatct ctcgagcgag cctgcttctt tgggtggccg cgtgctgaaa tacgataccg
     1441 gggataccgc gctgcaggtt accgggtggg gcggtctcac gctgtacacg atgcaggcca
     1501 agggcgggct gcctgccgag tccaacgatg cggaccagag cgtcgaaccg gccccggtgg
     1561 gtccgaagga ggtcaaggcg ttcgccgcaa aactcgcgca ggacctctcg gccagcgagg
     1621 atttcgcggt cggcttcgcc gcggattggg acaatctcgc aaagattgac gaactgcgcg
     1681 cgctcggctg cgatgccatg cgcaacgtga cctacgctct caaggaactc gccgatagcg
     1741 gcaggcgcgc cctcatttcc gatcgcatcc atatcatcag ggtcattcag ggacccaaac
     1801 ccgccgtgac gatgcagaaa ggcattctga ccgtcaccgt cgccccgcaa ttgggactgt
     1861 cggcccggcc atcctcgctt gccatcgtcc aggtgctgcg cgcctcattc tagcggcggc
     1921 cagccgcccg gcgcggtttc gcgcgtcccg atgtcatcgg cgtcattgcg aacgctcgcc
     1981 gagaaaaggg ttcggcgcac ctttggcgat gagcacggcg aaccccgccg ccacgccgcg
     2041 cgcatccgcc aacgcatcgt gccggcgccc atgaaagacg gcccctgcga gttccgccac
     2101 gtcgcaggcg cgcttgccct tgaggtcgat cccctgagcc gtgaaccagg gaatcgcatt
     2161 ggtgtaattg ggaagcggga gcgcatgcgc ccagccatag agcttcaaat tgccggtcag
     2221 gatcagatcg tcgcggccat gggcgaaagt ctcgctccca cccacgaaat ccaaaaacgc
     2281 acggtaggcc gtgatgaaat ccacgccacg gtggtccatg gccgcgttgg tgatgccggt
     2341 cagtgcggtc aaatagtccg aaagcaccgg attgaccctg ggccgcacca gcatgtcgaa
     2401 gcaatcaatc tccgtcaggc tcgctgcgtc gagcttcgtt gcgccgatct ggaccacttc
     2461 gctaagttcg ccgggccgtg tccagcgcga ttccagcgaa ccttcccagg cggtgaattc
     2521 gaggtcgaac accaccgcat gcacatgtct tcgttccttc actcgcttct ccgcacgtcg
     2581 ttaagaaatc gtaacattct ccgtttaaca gtctttcctg ccgtctaccg cggcgcgaag
     2641 cggtttgcac acgctttcct aagttctaaa agctctaatg cctcaccgcc ggaagatatg
     2701 cgcgggtcgg gaggattaca tggctcggac atcatcggtg gtccagacaa tgcacggtgc
     2761 cgctgcagag gaagcccgtt tcgaggccgc ggatgcgggc gcccatctgg cgaccgcatc
     2821 ccatctgatc ggcacgggtg agccggagct cgccgcgttc ttcaccgcgt tcacgcgcta
     2881 cgcctcgccg gaggatctga tccactacac cggcgcagaa ctggcgtcgt tggtgagtgg
     2941 ccttcatgcc cgcctcgcgc gccgccttgc cggcacgtcc ctggtgcaga tttttgctcc
     3001 ttcgtccgag gatgccgggt tcgcgcgaaa cgcggaaacc gtcgtccttg cggtcaatga
     3061 cgatattcca ttcctctacg attcttgcac cgccgagatt cgttcccgcg gctaccggat
     3121 cgcggcagcc ttccaccccg tggtggcgct cgcccgcgat gcgtccggga ccctctcgcc
     3181 caaaggttcg ccgctcaccg aaagcgtcat tgtgcttgcg ctcgagggtg tgctggacgc
     3241 ggaagaagcg gcagacttgc gcgacgggct catcgaggtc ttcgccggca cgcgcgccgt
     3301 ggtgcgcgac tggaagccga tgctggagcg catcgccgaa acggcggcct cgctgaaccg
     3361 caatccgccg caggtctccg aagccgaact ggaagagaac atcgccttcc tcgactggat
     3421 ggcggatgaa catttcacct ttctaggctg ccgcgactat gtgttctccg gcgagggcga
     3481 aggccgcctc gatccggtct atgaaagcgg attgggattg ctcgccgatc ccgaaatgcg
     3541 cgtggtgcgg cgtggacagg accgttcacg gctgacgcct gcggtgcgcg agttcctgaa
     3601 gaagccgacc ccgctcatca tcaccaaatc cgcgctgcgc tctccggtgc atcgtcgcgt
     3661 gcacatggac tatgtgggtg tgaagacctt cgatccgaag ggccggctca ccggcgaacg
     3721 gcgcttcatc ggccttttca cctccaacgc ctacagccaa ttgcccgtgg ccattccgct
     3781 gcttcgccgc aagaccgccg ccgtgcgggc aggctccgga ttgccgccat cgagccatga
     3841 cggcaaggcg ctgcagcata tcctcgacac attcccccgc gacgagctgt tccagatttc
     3901 cgaggacgag ctgctggcga ccgcgctcgg catcctcaat ctcggacaac ggcctaaggt
     3961 gcggatattc ctgcgcttcg accgcttcga ccgctatgtc tccgcactcg tcttcattcc
     4021 gcgcgaacgc tacagcggcg cggtgcgcga cacagtccat gccatcctcg cgcgcgcctt
     4081 caacggccgc cagtcggcgg ccacaccgat gctggacgat gaggcgctcg cacgcgtgca
     4141 ctacatcgtg ggccgcaacg aaggcccgag acccgaggca gacgtcaaag agctggaatc
     4201 cgaaatccgc agcgccatcc gcacctggga cgacggcttt gccgatgcgg cccgtttcgc
     4261 ccatggcgag atcaccggcg gagtgtcgct gcgctatgcg gcggcttttc ccgccggcta
     4321 tcgcgacgcc ttcacgcccg ccgaggccgt cgaggatatc ggccacatcg aaacggtatt
     4381 gcggaacgag ggcgcggcag gcccccttgc cgcccatttc tacagcagaa acggcgatgt
     4441 gcccgatgcc ctgcatttga aacttttcgt gcacggtctt ttcgtgccgc tctccgaatg
     4501 cctgccggtt tttgaaaatc tcggcctcag ggtcatcgcc gaggatgcct tcgccttggc
     4561 gccgcttgcc gaagacggca caggccaatc ggtcgctctg cagaatttcc tgatggcgcg
     4621 gagcgacggc aagccggtcg agatcgagcg tttacgaccg ctgctcgaag atgcgttcca
     4681 cgcggtctgg cgctctgacg cggaatcgga cggattcaac cggcttgtga ccgcggccgg
     4741 gctcgactgg cgcgatgttg tgatcctgcg tgctatcgcc aaattcctgc gccaggcggg
     4801 ccttgccttg agtcaaacct atgtggagag cgcgctcgcc aagaatccgg cactcgccgt
     4861 cttcttggtg cagctcttcc ggacacggca cgaccccgaa gcgttcgaga ccgccgcgcc
     4921 gcgcaaccgc gcagccgacg agattcgcaa gaccatccaa accgcgctgg acgatgtgcc
     4981 gagcgccgat gacgatcgca tcattcgcgc catgctgtcc gtcatcgatg cgatgctgcg
     5041 cacgagcgcc ttccagaggg acgcgtcagg aaatccccgg tcctatcttg ccttcaagct
     5101 tgagagcaaa cgtctcgaca tgctgccggc gcccaagccg ctctacgaga ttttcgtcta
     5161 ttcgcccgag gtcgaaggcg tgcatctgcg tttcggaaaa atcgcgcgcg gcggtatccg
     5221 ctggtccgat cgcgccgagg attttcgcac cgaaatcctc agtctcgcca aggcgcagca
     5281 ggtgaagaat tctgtcatcg tgccggtcgg cgcgaaaggc ggtttctatc cgaagacgat
     5341 tccagccggt gcggcgcgcg aggtggttca ggcgacaggc atcgccgcct acaagacctt
     5401 catcggcgcg cttctggacc tcaccgacaa tatcggtccc ggcggcagcg tcatctcccc
     5461 cgcccatacg ctgcgctacg atggcgatga tccctatttc gtggtcgccg ccgacaaggg
     5521 c
//
LOCUS       DASYEG010000250         6239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1104169, whole genome shotgun sequence.
ACCESSION   DASYEG010000250 DASYEG010000000
VERSION     DASYEG010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104169"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VGM72_10835"
     CDS             <1..710
                     /locus_tag="VGM72_10835"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEY4265805.1"
                     /translation="ARSYVLSLLPAPIAASTPQTEWFIVPSARVGGDAFGYGELDEGK
                     FVSFLFDVSGHGIGAAMLSVSILNVLRQHALPGCDFKSPAQVLARLNAMFQMENHNGM
                     FFTIWYGVYDRRQHQMTYASAGHHPAYLVSPNRTKITPLKTEGALIGVLEEFSVRENT
                     IDVPQNAALHLFSDGVFEVDDVKGRQLGLASLLPWLQQPPVDGRSEPQRIYQSVREFA
                     RSGPLDDDFSYLVVKFQ"
     gene            720..1058
                     /locus_tag="VGM72_10840"
     CDS             720..1058
                     /locus_tag="VGM72_10840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY4265806.1"
                     /translation="MEIEILLTGNIARIRLRGRLDTPGVDRIESQFTSSVVPDGRNTV
                     VDLREVSFIASMGLRMFIGIAKQLRRQNARMVLFAPQSQVSEVFHTVLLREIVPIVAD
                     EAEAMRYAAT"
     gene            1084..1545
                     /locus_tag="VGM72_10845"
     CDS             1084..1545
                     /locus_tag="VGM72_10845"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4265807.1"
                     /translation="MMRTMPDNSNRSDETNRDWSIAMRNDLTGLKEGLNGLGAWLASH
                     NVNTDAENRARLVFEEIVTNVIRYGFDDGGAHTIRANAQLKDGDLKLSFDDDGRSFDP
                     TTAPAPAPDYSLGQASIGGRGLILVRAAARALDYERTKDGRNRLTVTLARQ"
     gene            1661..3394
                     /locus_tag="VGM72_10850"
     CDS             1661..3394
                     /locus_tag="VGM72_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY4265808.1"
                     /translation="MTATGSEPTKIGRYEIEGLVGEGAMAKVYRARDPDIARVVAVKV
                     LKVELCIDEDYVSRFLREAKAAGAISHPNIVTVFDVGRFGDAPYITMEFLDEKSLADA
                     IAEHEKLPIKRVIAIGIQMARALDLAHRRGIVHRDVKPGNILLMEKGEVVKITDFGIA
                     RLDRSEDLNRTHAGTVLGTPRYMAPEQAAGRAVDGRADLFSLGVILYELLTGKKTFDS
                     NNVATLMLQIMQKDPEPIRDVAPDVPVSLQGVISKLLQKRPEQRFQTGAQLAEALERE
                     LAAIAAREEDAKRNRFIPLRVRWAMSAGAGLAMVFLITLGVVYYVERGIIRAQVIDSG
                     AAIAKFIATETAVQVLSQNWVPLELFVKDASERGSFDYLAVTDHDHVVKAATSPDIVG
                     KRFTPPNATLIVKAADFTASEVELPGGKAAFLFDTPILFQKTEIGRIYLGINRVGLDN
                     VLRSTLLLMSNIGILTVLSAVGMLYVFGGLIGRPLKLLTNAMRDFGQGDLDRRIAETR
                     NDEFGQLFSAYNHMADAVQAHIAHDTGARVAPETGAVMDTAVLGHTDANAEATLLTAS
                     VRAQPSPEPAS"
     gene            complement(3400..4536)
                     /locus_tag="VGM72_10855"
     CDS             complement(3400..4536)
                     /locus_tag="VGM72_10855"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4265809.1"
                     /translation="MSCDRADLRASDWSRKRRRLPQLLLLFPVLGLLAACMMKPASVP
                     PPMMAMIEPPPQQGPASIVLPVLQPTPGLAARERFQLAINQLQQGDSAHAAVELRAYL
                     AEIPNSVPAKTLLSQIETPIDMLYPADNFTIQLGRDETLSSLAGLYLGDVLGFYGLAR
                     YNMIDNPSRLVIGQSIRIPRTPQTLAAQAMRGTLQQASATPPLAGATANAPPRATILR
                     DPWPTIRQAVAQGRFDDAIKEAETRKVRPDTAQAAVLAAAYAGNARALRMANAPQAGT
                     QALRAGQLYLENANRPEDALEPLRLAIAINPADARAQMLLTTAKTKAADIHYRAGVAA
                     FQKQDLDGAIAAWDKTLAIDPDHKNAQLNRAQAVELKQNLQKLR"
     gene            4738..5457
                     /locus_tag="VGM72_10860"
     CDS             4738..5457
                     /locus_tag="VGM72_10860"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEY4265810.1"
                     /translation="MSFRSVARTHPGAVRPCNEDAVLQRAEAGIWAVSDGMGGHAAGD
                     VASALVIDSLMTLAPNGNNGTSMDAVRDTLARANGELYARGALVSPDHTMGATVTVLG
                     TGARNFYCLWAGDSRLYRFRGGTLTRLTRDHRYVQALIDSGLLDEEAAEKHPRRSVIL
                     RAVGVDAELELDVCEGTIEPGDVFLLVTDGVSGVCRDDELTEILSGRGLEDAADRIVE
                     QCLQRGAPDNLSLVLVAAAKD"
     gene            complement(5465..>6239)
                     /locus_tag="VGM72_10865"
     CDS             complement(5465..>6239)
                     /locus_tag="VGM72_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265811.1"
                     /translation="SYNIAKLQESYEVLSRHLYQLKKTDADISQFSGILARSIKYIVD
                     TTRDYCRNAPPAERQRLYAQADAPSDLSVLDSIAEMVISVLENTSHEFSGFDDKFGNM
                     AREIWDSVLPRFGTHAPGMDPLQQRFVLRLIEKIRENMEGWYSPLSRQALAVIGPYAA
                     KAESGERTAFKLCRDLFYQEFQDYPAFYEKDGERAKTLLPDNVRYEPETATLIHRYSF
                     GDEDRTILPALEIAPVTLSAESIGFIPPAGSTSPALRSA"
BASE COUNT         1169 a         1823 c         2010 g         1237 t
ORIGIN      
        1 aggcacgcag ctatgtgctt tcgcttctgc ccgcgcccat cgccgcctcc acgccccaga
       61 ccgaatggtt catcgtgcct tccgcgcgcg ttggcggcga tgccttcggc tatggcgagc
      121 tggatgaagg caaattcgtc agctttctct tcgacgtgtc ggggcatgga atcggagcgg
      181 cgatgctgtc ggtttcgatc ctcaacgtgc tcaggcagca cgcgctgccc ggttgcgatt
      241 tcaagagccc ggcacaggtg cttgcccggc tgaatgccat gttccagatg gaaaatcaca
      301 atgggatgtt cttcaccatc tggtacggcg tctacgaccg gcggcagcac caaatgacct
      361 acgcttcggc cggtcatcat ccggcctatc tggtttcgcc gaaccgcacg aagatcacgc
      421 cgttgaaaac cgaaggcgcc ttgatcggcg tgcttgagga attcagcgtc agggagaaca
      481 ccatcgacgt gccccaaaat gcggcgttgc atcttttcag cgacggggtg ttcgaggtcg
      541 acgacgtcaa aggccgtcaa ttgggcctcg cgagtctgtt gccttggctc cagcagccgc
      601 cggtggatgg gcgcagcgaa ccccaacgga tctatcagag cgtgcgtgaa ttcgcgcgtt
      661 ctggcccctt ggatgacgat ttttcctatc tcgttgtgaa gttccaatag ggcggtgaaa
      721 tggaaatcga aattttgctg acggggaaca tcgcgcgcat aaggctgcgc ggacgtctcg
      781 atacgccggg cgtcgacagg atcgagtcgc aattcaccag ttcggtggta ccggacggaa
      841 ggaataccgt cgtcgatctt cgggaggtca gtttcatcgc gtcgatgggg cttcggatgt
      901 tcatcggcat tgccaagcag ttgcggcggc aaaatgcccg gatggtgctg ttcgcgcccc
      961 aatcgcaggt cagcgaggtt ttccacaccg tgctcttgcg cgaaatcgtg ccgattgtcg
     1021 ccgacgaagc cgaagccatg cgctatgccg cgacttagct cgcttaccgg tgcgaagaac
     1081 gcgatgatga ggacgatgcc ggacaattcc aatcgcagcg atgaaaccaa tcgcgattgg
     1141 tcgattgcga tgcgcaacga tctcaccggc ttgaaggaag gtttgaacgg gttgggggcg
     1201 tggctcgcga gtcataacgt caacacagat gccgaaaacc gggcgcgtct ggtcttcgaa
     1261 gaaatcgtga ccaatgtgat ccgttacgga tttgacgatg gcggcgccca taccattcgc
     1321 gcaaacgccc agcttaagga cggggatctg aaactcagct tcgacgatga cggcaggtcg
     1381 ttcgacccga cgacggcacc ggcaccggcg cctgattatt cgctcgggca ggcgagcatc
     1441 ggcggacggg gtttgatcct cgtgcgcgcg gctgcgcggg cgctcgacta tgaacgcaca
     1501 aaggatggcc gcaaccggct gacggtgacg ctggcaagac aatagatcgc gcgaaaatcc
     1561 cacgcgaccg gcggcattgc gcgcaatccc ggcgaacaga tatcatgcgc gggggcgccg
     1621 cctgcggcgc gtgcgctatg catgggctgg aacggatttg atgacggcga caggaagcga
     1681 gcccacaaaa atcgggcgtt atgaaatcga aggcctggtc ggcgaggggg cgatggcgaa
     1741 agtctatcgc gcgcgcgatc ccgatatcgc gcgtgtcgtc gcggtcaagg tcctgaaggt
     1801 cgagctttgc atcgatgagg attacgtcag ccgcttcctg cgcgaggcaa aagcggccgg
     1861 cgccatctcg catcccaaca tcgtcaccgt gttcgatgtc ggccgtttcg gcgacgcgcc
     1921 ttacatcaca atggaatttc tcgatgaaaa gtctcttgcc gatgcaatcg ccgaacatga
     1981 aaaactgccg atcaagcgag tgatcgcgat cggcattcag atggcgcgcg cgctcgatct
     2041 tgcccatcgc cggggcatcg tgcaccgcga cgtcaagcct gggaacattc ttctcatgga
     2101 gaagggcgag gtggtcaaga tcaccgattt cggcattgcg cgcctcgacc gttccgagga
     2161 tttgaaccgg acgcatgccg gtacggtcct tggaaccccg cgttatatgg cgcccgaaca
     2221 ggccgcgggc cgcgccgtcg atgggcgtgc cgacctcttt tcgctgggcg tcatcctgta
     2281 cgaactccta acgggtaaga aaaccttcga cagcaacaat gtcgcgacgc tgatgctgca
     2341 gatcatgcag aaggaccccg agccgatccg cgatgttgcg ccggacgtgc cggtgagtct
     2401 gcagggcgtc atttcgaaat tgctgcagaa gcggcccgaa cagcgcttcc agaccggcgc
     2461 gcaattggcc gaagcgctgg agcgcgaact cgcggccatc gccgcgcgcg aggaagacgc
     2521 caagcgcaat cggttcatcc ccttgcgcgt acgttgggcg atgtccgcgg gcgcggggct
     2581 cgcgatggtg ttcctcatca cgctgggcgt cgtgtattac gtcgagcgtg ggatcattcg
     2641 tgcgcaggtg atcgattccg gcgcggcgat cgcgaaattc atcgccaccg aaaccgcggt
     2701 gcaggtgctc agccagaatt gggtgccgct ggaattgttc gtcaaggatg cgagcgaacg
     2761 cggcagtttc gattatctcg ccgtgacgga ccacgatcac gtcgtgaagg cggcgaccag
     2821 tccggacatt gtgggtaagc gtttcacgcc gcccaatgcc acgctcatcg tgaaggcggc
     2881 ggatttcacg gcctccgaag tcgaattgcc gggcgggaag gcggcgttcc tgttcgacac
     2941 gccgattctt ttccagaaga cggagatcgg ccggatctat cttgggatca atcgcgtcgg
     3001 gctggacaat gtgctgcgct cgacgctgct cctgatgtcc aacatcggca tcctgacggt
     3061 cctgtcggcc gtcggcatgc tgtatgtgtt cggcggtctg atcggcaggc cgctcaagct
     3121 cctgaccaat gccatgcgcg atttcgggca gggcgacctc gaccggcgta tcgccgaaac
     3181 ccgcaacgac gaattcggcc agttgttttc cgcctacaat cacatggccg acgcggtgca
     3241 ggcccatatt gcgcacgata ccggcgcgcg tgtcgcgccg gagaccggtg cggttatgga
     3301 caccgctgtt cttggccata cggatgccaa tgcggaagcg acacttctga ccgcctcggt
     3361 tcgcgcgcag ccatcaccag aacccgcgtc ctgaacggtt cagcgcagtt tttgcagatt
     3421 ctgtttcagc tcgacagcct gtgcacgatt gagctgcgcg ttcttatggt cgggatcgat
     3481 ggcgagcgtt ttgtcccagg ccgcgatggc accgtcgaga tcctgtttct ggaaggcggc
     3541 gacgccggcg cgataatgaa tgtcggcggc tttggttttg gcggtagtta gcagcatctg
     3601 cgcacgcgca tccgccggat tgatcgcgat ggcgaggcga agcggttcca gagcgtcctc
     3661 cggccggttc gcgttctcca gataaagctg ccccgcgcgc aacgcctgag tgcctgcttg
     3721 cggcgcgttg gccatccgca gcgcgcgcgc attgccggca taggccgcgg caagcacggc
     3781 cgcctgtgcg gtatcgggcc ggactttgcg ggtctcggct tccttgattg catcatcgaa
     3841 gcgcccttgg gcgacggctt ggcgaatggt gggccaggga tcgcgcaaga tcgtcgcgcg
     3901 cggcggcgca ttcgccgttg ctccagccag gggcggtgtt gcgctggcct gctgcagggt
     3961 gccgcgcatc gcctgggcgg caagcgtctg tggcgttctc ggaatgcgga tgctttgtcc
     4021 gatgacgagc cgggagggat tatcgatcat attgtagcgc gcgagcccgt aaaatccgag
     4081 cacgtcgccg agatagaggc cagcgagcga agacagtgtt tcgtcgcggc ccagctgaat
     4141 cgtgaaatta tccgccggat agagcatatc gattggcgtc tcgatctggg agagcagggt
     4201 tttggcgggc acgctattgg ggatttctgc gagataggcg cgcagctcga ccgcggcgtg
     4261 cgccgaatca ccctgctgca attggttgat cgcgagttgg aaacgctcgc gcgcggcgag
     4321 gccgggcgtc ggctgcaaaa ccggcaggac gatagaggcg ggcccctgtt gcggcggcgg
     4381 ttcgatcatc gccatcatcg gcggcggtac ggatgccggt ttcatcatgc acgccgcaag
     4441 caaacccaat acgggaaaca acaacagcaa ttgcggaagc cggcggcgtt tcctgctcca
     4501 gtccgaagcg cgcaggtcag cccgatcgca cgacatttcc tgttccccgc cgaggttctc
     4561 acccattcgc tatgattcgc tgcatcgcac caaacgtcaa gcgcgcggcc gggtatcgcg
     4621 tgccggagcc ttcggatata gtgcatgtga catagtgata cgctccgtcg cggcgatggc
     4681 gaattaccca cagcacgctg cttttatgcc cctgtattcc tcatgaacga tgggaaaatg
     4741 agcttccgat cggttgcccg cacccatcct ggcgctgtgc ggccatgtaa cgaagacgcg
     4801 gtgctgcaac gcgccgaggc gggaatctgg gctgtctccg atggaatggg cggtcatgcc
     4861 gcgggcgatg tggcgagcgc cctggtgatc gattcgttga tgacgctcgc gcccaacggc
     4921 aacaatggca cctccatgga tgccgtgcgc gacacgctgg cgcgcgcaaa cggcgagctt
     4981 tacgcgcgcg gtgcgctggt ctcgcccgat cacacgatgg gcgcgaccgt tacggtgctc
     5041 ggaaccggcg cacgaaattt ctactgcctg tgggcggggg attctcgatt gtaccgcttt
     5101 cgcggcggca cgctgacgag gctgacgcgc gatcatcgct atgttcaggc gctgatcgat
     5161 tcaggcttgc tcgacgaaga agccgccgaa aagcatccgc gccgcagtgt catcctgcgt
     5221 gcggtgggag tcgatgccga actcgaactt gatgtttgcg agggcacgat cgagccgggc
     5281 gatgtgttct tgctggtgac cgatggggtg agcggtgtgt gcagggacga cgaactcacg
     5341 gagattttgt ccgggcgcgg actcgaagat gccgccgacc gcatcgtgga acagtgcctt
     5401 cagcgcggcg cgcccgacaa tttgtccctg gtgctggtcg ccgccgccaa ggattagatc
     5461 gtgatcacgc ggagcgcagc gcagggctcg tgctgccggc gggcggaatg aagccgatcg
     5521 actccgcgct caatgtgacg ggcgcgatct cgagcgcggg caggatcgta cgatcttcat
     5581 cgccgaaact gtagcggtgg atcagcgtgg cggtctccgg ctcgtaccgc acattgtcgg
     5641 gaagcagcgt cttggcccgt tcgccgtcct tttcgtaaaa ggcaggatag tcctggaatt
     5701 cctgatagaa cagatcgcgg cacagcttga acgcggtgcg ttcgcccgat tcggctttgg
     5761 cggcataggg gccgatcacc gccagtgcct gacgtgacag cggcgaatac cagccttcca
     5821 tattttcgcg aatcttctcg atcagtcgca gcacgaaacg ctgttgcaga gggtccatgc
     5881 cgggggcgtg cgtgccgaag cgcggcagca cgctgtccca gatctcgcgc gccatgttcc
     5941 cgaacttgtc gtcgaatccg ctgaattcgt gagacgtgtt ctccaacacc gagatcacca
     6001 tttcagcgat ggaatccagc acgctgagat cgctcggcgc atcggcctgc gcgtagagcc
     6061 gctggcgctc cgcaggtgga gcgttgcggc aataatcgcg cgtcgtgtcg acgatatatt
     6121 tgatggagcg ggcgaggatg cctgaaaatt gcgagatgtc cgcatcggtc ttcttcagtt
     6181 gataaagatg ccggctcaac acctcgtagc tctcctgcaa tttcgcgatg ttataggaa
//
LOCUS       DASYEG010000251         2227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_671384, whole genome shotgun sequence.
ACCESSION   DASYEG010000251 DASYEG010000000
VERSION     DASYEG010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671384"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /gene="bshC"
                     /locus_tag="VGM72_10870"
     CDS             <1..540
                     /gene="bshC"
                     /locus_tag="VGM72_10870"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis BshC"
                     /protein_id="HEY4265812.1"
                     /translation="SQVIYAKLLGRQPVAFPRAGFTLLDERAAKRMRRYELPLTGLFT
                     NDQALRETMSARLVPPELRQRLTATEETVASALETLSKELGRFDVSLNGALGTSRRKI
                     EYQIGKIGRKTASQIMARDEQAAADAKALSGLVYPEKHLQERLYSIVPFLAKFGPGLI
                     GDIYNSVDTSNPDHRILTF"
     gene            complement(548..>2227)
                     /locus_tag="VGM72_10875"
     CDS             complement(548..>2227)
                     /locus_tag="VGM72_10875"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEY4265813.1"
                     /translation="YILQAVPSAAEPPRSGKWYARWWQYRRVHRLLGWKFWSFVVGAA
                     PLDPALEEFWRKLGYVVIQGYGLTETAPVATLNHPFETRKGSVGKAIAGVEIRIAPDG
                     EVLLRGDNITPGYFGQPANSLRDDEGFLHTGDIGALDSEGRLSILGRKKEMIVSPDGL
                     NVYPEDVERVLNAIAGVRESAVVADKQGGRELVHAVLALTPGTSAESVMSEANSKLES
                     HQRVRGWSIWPGDALPRTSGTGKLKRGEVAAWLAGSGQTPALASPDGDSIEDVIRKFA
                     GGRAVSASTSLDDLGLSSLDRIQLLMELEKRTGAPIDETAFANARSVADLVQAKPAVS
                     TAAETPFEYPEWNRSAWARAVRRVMLPGLIIPLTRAFAWLTVSGLENLRDLKGPAIFA
                     SNHQSHFDAPVILSALPARWRYRISPAAAKEFFDLRSRFANALNYYLASLVFNIFPLP
                     QRESGAREAMRHAGALVAEGYSILIFPEGRRSEHGEIGHFQPGVGMLAARLRIPVIPV
                     RLTGLDHVLHKDAKFATPGKAGVRFGAPIRPEGDDPAAIAKQIEDAVRALA"
BASE COUNT          378 a          633 c          804 g          412 t
ORIGIN      
        1 tcgcaggtga tttatgcgaa gctgctgggg cggcagccgg tggcgtttcc gcgggcgggg
       61 ttcacgttgc tggatgagcg agcggcgaag cggatgcggc gttacgagct gccgctgacg
      121 gggctgttca cgaacgatca ggctttgcgc gagacgatgt cggcgcggct ggtgccgccg
      181 gaactgcggc agcggctcac ggcgacagag gaaacggtgg cgagcgcgct tgaaacgctg
      241 agcaaagagc tggggcggtt cgatgtgtcg ctgaatggcg cgctgggcac gagccggcgg
      301 aagatcgaat atcagatcgg gaagatcggg cgcaagacgg cgtcgcagat catggctcgc
      361 gatgagcagg cggcggcgga tgcgaaggcc ctctccgggt tggtttatcc cgagaagcat
      421 ttgcaggaac ggctgtattc gattgtgccg tttctggcga agttcgggcc ggggctgatc
      481 ggcgatatct ataattccgt cgataccagt aacccggatc accgaatact caccttttag
      541 gacttcatca agccagggcg cggacggcgt cttcaatttg ttttgcaatc gcggcggggt
      601 cgtcgccttc ggggcggatg ggtgcgccga agcggacgcc cgctttgccg ggagtggcga
      661 atttggcgtc cttgtggagg acgtggtcga ggccggtgag ccgcaccgga attacgggga
      721 ttcggaggcg tgcggcgagc atgccgacgc cgggctggaa gtgtccgatc tcgccgtgtt
      781 cgctgcgccg tccttccggg aagatcagaa ttgaatatcc ttccgcgacc agcgcgcctg
      841 cgtggcgcat ggcttcgcgc gcgccggatt cgcgctgggg cagcgggaag atgttgaaga
      901 ccagcgaggc gaggtagtag ttcagggcgt tggcgaaccg cgagcggagg tcgaagaact
      961 ctttcgccgc cgctggtgag atccgataac gccagcgcgc gggaagggcg gagagaatga
     1021 cgggcgcgtc gaaatggctc tggtgattcg atgcgaagat agccggccct ttgagatcgc
     1081 ggaggttttc gagccctgag accgtgagcc acgcgaaagc gcgcgtgagc gggatgatca
     1141 ggcccggcag catcacgcgg cgaacggcgc gcgcccaggc cgatcggttc cattcgggat
     1201 attcgaacgg cgtttcggcg gcagtgctga cggcgggttt cgcttgaacg agatcggcga
     1261 cgctgcgcgc gttggcaaag gcggtttcat cgatcggcgc gccggtgcgt ttttcgagct
     1321 ccatcaggag ctggatgcgg tcgagcgaac tgaggccgag atcgtcgagc gaggtactcg
     1381 cgctgaccgc gcggccgcct gcgaatttgc ggatgacatc ttcgatggaa tcgccgtctg
     1441 gcgatgcaag cgctggcgtc tgcccgctgc ctgccagcca ggcggctact tcgccgcgtt
     1501 tcagtttccc cgttccggaa gtgcgaggca gcgcgtcgcc aggccagatc gaccagccgc
     1561 gcacgcgctg gtgcgattcg agcttgctgt tggcctcgct catcaccgat tcggcgcttg
     1621 ttccgggcgt gagggcgagg accgcatgga cgagttcccg gccgccctgc ttgtcagcga
     1681 cgaccgccga ctcgcgcaca ccggcaatcg cgttcagcac gcgctccacg tcttcgggat
     1741 agacattgag gccgtcgggt gagacgatca tttccttttt gcggcccaga atgctgaggc
     1801 ggccttcgga atcgagcgcg ccgatgtcgc cggtgtggag gaaaccttcg tcatcgcgca
     1861 ggctgttggc gggctgtccg aaatagccgg gcgtgatatt gtcaccgcgc agcaacacct
     1921 cgccatcggg cgcgatgcga atttcgacgc cggcgatcgc cttgccgacg gaccctttgc
     1981 gcgtttcgaa gggatggttg agcgtggcca cgggcgcggt ttcggtgagt ccgtagccct
     2041 ggatcacgac gtagccgagc ttgcgccaga attcttccag cgcaggatcg agcggcgcag
     2101 cgcccaccac gaagctccaa aatttccagc cgagcaggcg atgcacgcgg cgatattgcc
     2161 accatcgcgc gtaccatttg ccggagcggg gcggttcggc cgccgagggc acggcctgga
     2221 ggatgta
//
LOCUS       DASYEG010000252         8718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_92007, whole genome shotgun sequence.
ACCESSION   DASYEG010000252 DASYEG010000000
VERSION     DASYEG010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 8718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8718
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92007"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1674)
                     /locus_tag="VGM72_10880"
     CDS             complement(<1..1674)
                     /locus_tag="VGM72_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265814.1"
                     /translation="MRKVRQSRQLFGRRIAGLCALLAALALSAISFAIAWNALQPASA
                     RLMSVDRSLLSTPAQVQDWLDQKDSWGPAYTAGPAWKKFMAMIHNEIKIMGLVNITDV
                     PFPYTRWYTSEFPDKSGWSLTSNGERVDVASYGTQSGSTGPLGVTAPMILYDLSLPVA
                     QRPPLSALAGKIVVVKQQPYDTLGTPKRMVLGVPAPQMPYAFCGNPPSCTPPLSGEAN
                     PSPQWGTPGPAMSYKDYEYRSGPESFPTPLFEKIPVSVESSFRNRDQFGQIREVITNV
                     LMPSGAAAAVTVMDLPALAAQGARIHPTPRRFNVPLLMLDRVAGTKVLSDAAAGKTGT
                     VVLNAHEEENANAFETVAVLPGRNFGKPGDQSILLATHVDGPSVVEDDGALAILSVLH
                     YYARIPQAERLKSITVYFESRHFVPGTEASYPFDTVKLRPELFKTVVGGLALEHFGGL
                     QFVEQDGAYIPSGLAATTYVWGWPNRLAIQEATTAIRDQELPRAINDVPARPGTNGKA
                     QQGWLGGGFSRYLVDLGGWPGWHISGDWPSAGFQAYYPASRTRVSAELFV"
     gene            1885..2535
                     /locus_tag="VGM72_10885"
     CDS             1885..2535
                     /locus_tag="VGM72_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4265815.1"
                     /translation="MKKAVPGGRVLFRDLELCLSPGAFVAIMGESGAGKSTLLNLIAG
                     LDVADEGSIAIRGTPLEGLDDDARTRLRREAIGFVFQAFHILPHLTLAQNVALPLVLQ
                     HMPAAESHARSTEMLTSVGLPGRGEDFPRALSGGELQRVAIARALVHRPSLILADEPT
                     GNLDPETAARILDLFAKEVRESGAAVLLVTHSHVAAAVADRTLTLTSTGLVERNNG"
     gene            2528..5113
                     /locus_tag="VGM72_10890"
     CDS             2528..5113
                     /locus_tag="VGM72_10890"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4265816.1"
                     /translation="MANAAAISRVATRWMIAGEWRAHPTRVIVAALAIAVGVALGFAV
                     HLINASALDEFARAVKTVNGDADLQVHAVTPLGFDETLYPRLARTPGIAAVSPVVELP
                     LTTGANVSLTLLGVDPFRAAMVTPSLIGRRIASAAQQPAPSGTDAQVSMADQAFDENA
                     VYLSQAALAATGKHIGETIEFFDGGNGVGFVIAGILPGVAEGQSLAVTDIAAAQWRFG
                     RLGRLQRIDVKLAEGSDVAQVRSALAAVLPADAEIVSETSEARRTDSLSRAYRVNLDM
                     LSLMALLTGAFLAFSAQALSVARRRSQFALLRVLGMQRRALLVQVLLEGGIVGGVGAG
                     AGLGLGLLLAQTALRFLGGDLGGRYFSGSKPNLIFAPGAALAFFALGVLAALIGSVLP
                     ARDAARAQPAVALKDAGDAADPRSPPGARIAFVLLSAGVAAAFLPAAGGLPLFGYFAI
                     ALLLAGGVAAMPLLARLLLAPLQHFAFRVPAHLASKHLWGAPSQAAIALCGIVASTSL
                     MIAMAVMVASFRTSVDNWLVQILPADIYLRVEGDDYSAFSPDVQQRLAATPGIAAIHF
                     RKLTPLRLSPDEAPIAWVAADIDPAAPGKTLPMIGAALRVPQGATPIWLSEPATWLYR
                     YRPGDVIALPLAKNGGMTKVFVAGIWRDYGRQQGSIAMNAKDYVRLTGDMMRSDAAVT
                     LAPGSAPSAVIEAMRARLAPSLAARVEFAPSRALRDRSLEIFDRSFAVTYVLEAIAIL
                     VGLTGVAATVSAQTLARTKEFGMLRHIGVLRRQIVAMLAAEGALLGAVGVIAGIGLGI
                     AISQVLIHVINPQSFHWTMETRLPLAVFAIVTIALMLASAGTALLAGRRALSSDAVRA
                     VREDW"
     gene            5113..6192
                     /locus_tag="VGM72_10895"
     CDS             5113..6192
                     /locus_tag="VGM72_10895"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid 1,2-hydratase"
                     /protein_id="HEY4265817.1"
                     /translation="MRALKIAVALLLIVTGVPAAGAPQLPAYPTMRPGVMLHFPADHG
                     AHPEFRTEWWYITGWLDTADGRSLGFQVTFFRTRPATDERNPSAFSARQVLFAHAALS
                     DPSVGHLLHDQRVARAGFGLAQAATADMDVVLDDWTLKRGLDGRIHTKVGAKDFSLDL
                     NFAPAQPILLQGENGYSRKGPLPTEASYYYSLPHLTVSGNVERGGKSVAVTGTAWLDR
                     EWSSSYLDPQAVGWDWVGLNLDDGAALMAFQIRDAKGAALWAGGSFRDAKGTLTRFAP
                     EDVRFATVRNWHSPRTATDYPVERVLGVRLPTGPREWHITPLFDDQELDSRAAGGPVY
                     WEGAARVAGGRGYLELTGYFKPLKM"
     gene            complement(6194..7135)
                     /locus_tag="VGM72_10900"
     CDS             complement(6194..7135)
                     /locus_tag="VGM72_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265818.1"
                     /translation="MRFAGLIPALAVVFFSSAGWSGAAWAQSWDTYANRENFFSVNLP
                     GDPVMTQAPYKTAKGTTLTANIFTAIAPPGSRLSGTYTVTVVDFTKAKDEMSTAVEQA
                     RNAMRAKGTVKYDGLNNIDLHLSRRLTIETPMTRILAEILMAANNRLYITEAETALNV
                     PPPAIFQASLQILDDSGVRIRTRTALGEPEGVKNPIGAGGIVNEPDKIAAVATGAWHS
                     AGSACTPAYFKSGMRTKTKRDEDALSGTITNSGTTISGELILVGSRLGQFINPMTDKV
                     IMLFDPQDGNKLSISSIGEPALGWPDVTLDLCPGSRG"
     gene            complement(7222..8502)
                     /locus_tag="VGM72_10905"
     CDS             complement(7222..8502)
                     /locus_tag="VGM72_10905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY4265819.1"
                     /translation="MTSNDTHRTIDAIWRIEQARLIAGLARIVRDVGLAEELAQDALV
                     IALEKWPQNGIPENPGAWLMATAKHRAIDRLRRKKLLARKHEELGRELEIEQETAVPD
                     LDAALDDDIGDDLLRLMFTACHPVLSTEARVALTLRLIGGLTTEEIARAFLVPEPTIA
                     QRIVRAKRTLAEKAVAFEVPRGPELAPRLSSVLEAIYLIFNEGYAATTGEDWMRPQLC
                     EDALRLGRILAELAPREPEVHGLVALMEIQESRSRARTGADGAPVLLLDQNRARWDQV
                     LIRRGLAALARAEGLGGALGPYALQAAIAAYHARARSADETDWARIAALYGALSECAP
                     SPVVELNRAVAAGMAFGPAAGLEIADALNGERTLKTYYLLPAVRGDLLAKLGRNAEAR
                     AAFEHAASLTRNTRERAVLLARAQGCAAAPAPRE"
BASE COUNT         1417 a         2853 c         2875 g         1573 t
ORIGIN      
        1 cacgaaaagc tccgcgctga cgcgcgtccg cgacgccggg taataggcct gaaaacccgc
       61 gctcggccaa tcgccggaga tatgccagcc gggccagccg ccgagatcga cgaggtagcg
      121 cgagaagccg ccgccgagcc agccttgctg cgctttgccg ttcgtaccgg ggcgcgcagg
      181 cacgtcgttg atcgcgcgcg gcagctcctg atccctgatg gcggttgtgg cttcctggat
      241 cgccagccga ttgggccaac cccagacata ggtggtggcc gccagccccg acggtatgta
      301 ggcaccatct tgttcgacga attgcaagcc gccgaaatgt tcgagcgcaa gtccgccgac
      361 caccgtcttg aacagctcgg ggcgcagttt caccgtgtcg aagggatagc tggcttccgt
      421 gccgggcaca aaatgacgcg attcgaaata gacggtgatg ctcttcaagc gctcggcttg
      481 cggaattctc gcgtagtaat gcagcaccga gagaatcgcc agagcgccgt cgtcctcgac
      541 gacgctcggc ccatccacat gggtggcgag caggatggac tgatcgccgg gcttgccgaa
      601 attcctgccg ggaagcaccg cgacggtctc gaatgcgttc gcgttctcct cctcatgcgc
      661 gttcaacacc acggttccgg ttttccccgc cgcggcatcg ctcagcacct tcgtccccgc
      721 cacccggtcc agcatcagca gcggcacgtt gaaccgccgc ggggtcggat ggatgcgcgc
      781 gccctgcgct gcgagcgccg gcagatccat caccgtaacc gcagccgcag ccccggaagg
      841 catcagcaca ttcgtgatga cttcacggat ctgaccgaac tggtcacggt tgcggaagct
      901 ggattcgacg ctgacgggaa tcttttcgaa cagaggcgtg gggaagcttt caggacccga
      961 gcggtactca tagtccttat aggacatggc cggaccggga gtgccccatt gcggcgaggg
     1021 gttggcttcg cctgacagcg gcggcgtgca ggacgggggg ttgccgcaaa aggcataggg
     1081 catctgcggc gcgggcacgc cgagcaccat acgcttgggc gtgccgagcg tgtcataggg
     1141 ttgctgcttg acgaccacga tcttgcccgc gagtgccgaa agcggcggac gctgcgcgac
     1201 cggcaggctg agatcgtaaa ggatcatcgg cgccgtcacg ccgagcggcc ccgtggaacc
     1261 cgattgcgta ccgtaagacg caacgtccac acgctcgccg ttcgaggtaa gcgaccagcc
     1321 gctcttgtcg gggaattcgg aagtgtacca gcgggtgtag gggaacggga catcggtgat
     1381 gttcaccaat cccatgatct tgatctcgtt atggatcatc gccatgaatt tcttccacgc
     1441 cgggccggcg gtgtaggcgg ggccccagga atctttctgg tcgagccaat cctggacctg
     1501 ggcgggcgtg gacagaagcg aacgatcgac gctcatcaac cgggccgatg ccggttgcag
     1561 cgcgttccat gcgatggcga aggaaatcgc gctcagcgcc aacgcggcaa gcagcgcaca
     1621 gagaccagca atcctgcgcc cgaacaattg ccgggattgt cgtactttgc gcatgagact
     1681 cccgcgccac tcgcgcaacc ccatagaaca ggaccttccg ggacaacgca acggcagaat
     1741 ggagcgggtt taacgaaggc gcccggatgg atggacgtcc ggccggtgtc gcgccttcat
     1801 gcttaacgtc cggcgcgctt tcgtgcttaa aatgtgccga tgcgcgcgcc cgccttgtcc
     1861 tttcctctgc tttgcgtaac agcattgaag aaagcggtcc ccggcgggcg ggttcttttc
     1921 agagatttgg agctttgtct ctcgcccggc gcattcgtgg cgatcatggg cgaatccggc
     1981 gccggaaagt ctacgctgct gaacctgatc gcgggcctgg atgtggccga cgagggcagc
     2041 atcgcgatcc gcggtacacc gctcgagggg ctcgacgacg atgcccgaac gcggctgcgg
     2101 cgcgaggcaa tcggcttcgt cttccaggct ttccatatcc tgccgcatct gacgctggcg
     2161 cagaacgtcg cgctgccgct ggtgctccag cacatgcccg ccgcggaaag ccatgcccgc
     2221 agtacggaga tgctgacctc tgtcgggctt cccggacgag gcgaggattt tccgagagct
     2281 ctttcgggcg gcgagcttca acgcgtggcg atcgcacgcg cgctggtgca tcgcccgagc
     2341 ctgatcctgg ccgatgagcc gaccggcaat ctcgatcccg aaacggcggc gcgcatcctg
     2401 gacctgttcg cgaaggaagt gcgtgagtcc ggcgccgctg tgctgctcgt cacccattcc
     2461 catgtggcgg cggcggtcgc ggaccgcacg ctgacgctga cgagcacggg gctggtggag
     2521 cgcaataatg gctaacgcgg ccgccatcag ccgcgtcgcc acgcgctgga tgatcgccgg
     2581 cgagtggcgg gcccatccca cgcgcgtgat cgtggcggcc ttggcaatcg cggtcggcgt
     2641 ggcgctcggc ttcgccgtgc atctgatcaa cgcatcggcg ctcgacgaat tcgcgcgcgc
     2701 ggtgaaaacc gtgaacggcg atgccgacct gcaggtgcat gcggtgacgc cgctcggctt
     2761 cgacgaaacg ctttaccccc ggctcgcccg cacccccggt atcgcggctg tgagcccggt
     2821 ggtggaattg ccactgacca caggcgcgaa tgtttcgctg acgctgctgg gtgtcgatcc
     2881 gttccgggct gcgatggtga cgccgagcct gatcgggcgg cgcatcgcaa gcgcggcgca
     2941 gcagccggcg ccctccggaa cggatgcgca ggtttcgatg gccgatcaag ccttcgatga
     3001 gaatgcggtg tatctctctc aggcagcgct cgccgcgacg ggcaagcata tcggcgagac
     3061 aatcgaattc ttcgacggcg gcaacggcgt tggcttcgtc attgccggca ttttgcccgg
     3121 cgttgcggaa ggccagagcc tcgccgtcac cgacatcgcc gccgcgcaat ggcgcttcgg
     3181 cagattgggc cgcctgcagc gcatcgatgt gaagctcgcc gagggaagcg atgttgcgca
     3241 ggtgcgcagc gcattggcgg ccgtcctgcc ggccgatgcc gaaatcgtca gcgaaacctc
     3301 cgaagcgcgg cgaaccgatt cgctcagccg cgcctatcgc gtcaatctcg acatgctgtc
     3361 gctgatggcg cttctgaccg gcgcgttcct cgccttttcc gcacaggcgc tttcggtggc
     3421 gcggcggcgc tcgcaattcg cgctgcttcg cgtgctgggc atgcagcgcc gcgcacttct
     3481 cgtgcaggtg ctgctggaag gcggcatcgt cggcggcgtc ggcgcgggcg cgggtcttgg
     3541 actggggctg ctgctcgctc agaccgcgct gcgctttctc ggcggcgacc tcggcggacg
     3601 ttatttcagc ggctccaaac cgaacttgat cttcgcgccg ggcgccgccc tggcgttttt
     3661 cgcgctcggc gtgctggcgg ctttgatcgg cagcgtgctg ccggcgcgcg atgccgcccg
     3721 cgcccagcca gcagtggcgt tgaaggatgc cggcgacgcg gccgatccca gatcgccgcc
     3781 cggcgcgcgc atcgcgttcg tgttgttgag tgccggggtc gccgccgcgt ttcttccggc
     3841 cgccggcggc ctgccgcttt tcggctattt cgccatcgcg cttctgctcg caggcggggt
     3901 cgccgccatg ccgctgctgg cgcggctgct gctcgcgccg ctgcagcatt tcgcgttcag
     3961 ggtgccggcg catctcgcat cgaaacatct gtggggcgca ccgtcgcaag ccgcgatcgc
     4021 gctctgcggg atcgtcgcaa gcaccagcct gatgatcgcc atggcggtga tggtggcgag
     4081 cttccgcacc tcggtcgaca attggctggt gcagatcctg cccgccgata tttatctgcg
     4141 cgtggagggc gacgactaca gcgcgttttc gccggacgtg cagcaaagac tggccgcgac
     4201 gccgggcatt gcggcaattc atttccgtaa actgacgccg ctcaggctct cgccggacga
     4261 agcccccatc gcgtgggtgg cggccgacat cgatcccgcc gctccaggca agaccctgcc
     4321 gatgatcggc gcggcactcc gcgtgccgca aggcgccacg cccatatggc tgtcggaacc
     4381 cgcgacctgg ctctatcgct accgccccgg cgatgtcatc gcgctgccgc tcgcgaagaa
     4441 cggcggcatg acgaaggttt tcgtcgccgg gatctggcgc gactacggac gccagcaagg
     4501 aagcattgcc atgaacgcca aggattatgt gcggctcacc ggcgatatga tgcgttcgga
     4561 tgcggcggtc acgcttgctc ccggttctgc gccctcggcg gtgatcgagg cgatgcgcgc
     4621 gcggcttgcg ccctcactcg ccgcacgcgt ggaattcgcg ccctcgcgcg cgctacgcga
     4681 caggtcgctg gagattttcg accggagttt cgcggtgacc tatgtgctgg aggcgatcgc
     4741 gattctcgtc gggctgaccg gcgtcgccgc gaccgtttcg gctcagacgc tggcgcgcac
     4801 caaagagttc ggcatgctgc gccatatcgg cgtgctgcgc cgtcaaatcg tcgcgatgct
     4861 cgccgccgaa ggcgcgctgc tgggtgcggt gggagtgatc gcagggatag ggcttggcat
     4921 tgcgatcagc caggtgctga tccacgtcat caatccgcag tccttccatt ggacgatgga
     4981 gacccggctt ccgctcgcgg tgttcgccat tgtgacgata gccctgatgc tggcctccgc
     5041 cggaacggcg ctgctggcgg gaaggcgcgc gctctcctcc gacgcggtgc gcgcggtcag
     5101 ggaggattgg tgatgcgggc gctgaagatc gcggtcgcat tgctgctgat cgtcacgggc
     5161 gttcctgccg ccggcgcacc gcaactgccg gcctatccca cgatgcgccc cggcgtgatg
     5221 ctgcatttcc cggccgatca tggcgcccac cccgaattcc gcaccgagtg gtggtacatc
     5281 accggatggc tggacaccgc cgatgggcgc tcgctgggat tccaggtgac cttcttccgc
     5341 acgcgccccg ccaccgacga aagaaatccg agcgcgttca gcgccaggca agttctgttc
     5401 gcgcatgcgg cgctctccga tccgtcggtc ggtcatctgc tgcacgatca gcgcgtggcg
     5461 cgcgccggtt tcggtttggc gcaagccgcg acggccgata tggacgtggt gctggacgat
     5521 tggacgctca aacgcggttt ggacggccgc atccacacca aagtgggagc taaggatttc
     5581 tcgctcgatc tgaacttcgc cccagcccag ccaatcctgt tgcaaggcga gaacggctac
     5641 agccgcaaag gcccgttgcc gacggaagca agctattatt acagccttcc ccatctgacc
     5701 gtctcgggaa acgtggagcg cggcggcaaa tccgtcgccg tgacaggcac cgcatggctc
     5761 gatcgcgaat ggtcgtcgtc ctatctcgat ccccaagcgg tgggttggga ttgggtgggg
     5821 ctcaatctcg atgacggggc cgcgctgatg gcgttccaga tccgcgatgc gaagggggca
     5881 gcgctgtggg cgggcggctc gttccgcgat gccaagggta cgctgacgcg tttcgcaccc
     5941 gaggatgtgc gcttcgccac ggtgcgcaat tggcattcgc cgcgaaccgc gaccgattat
     6001 ccggtggagc gcgtgctcgg cgttcgcctt cccaccggcc cgcgcgaatg gcacatcacg
     6061 cctttgttcg acgatcagga attggatagc cgtgccgccg gaggcccggt ctattgggaa
     6121 ggcgccgcgc gcgtggccgg cgggcgcggc tatctggaac tcaccggcta tttcaagccg
     6181 ctgaagatgt gattcagccg cggcttcccg ggcacagatc gagcgtcacg tccggccagc
     6241 ccaaagcggg ctctccgatg gacgaaatgc tgagcttgtt gccgtcctgc gggtcgaaca
     6301 gcatgatcac cttatcggtc atcgggttga tgaattggcc caggcgcgag ccgaccagaa
     6361 tcaactcgcc ggaaatggtc gtccccgaat tcgtgatcgt gccggaaagc gcgtcttcgt
     6421 cgcgcttggt cttggtgcgc atgcccgatt tgaaataggc cggcgtgcac gcgctgcccg
     6481 cgctgtgcca tgcgccggtc gccacggcgg cgatcttgtc gggttcgttg acgatcccgc
     6541 cggcgccgat cgggtttttc accccttcgg gttctcccaa cgccgtccgg gtccgaatgc
     6601 gcacgccgct gtcgtcgagg atctgcagcg aggcctggaa gatggccgga ggcggcacgt
     6661 tcagcgctgt ttcagcctcg gtgatgtaaa ggcgattgtt cgccgccatc aggatttcgg
     6721 cgaggatgcg cgtcatgggc gtttcgatcg tcagacgccg gctcaaatgc aggtcgatat
     6781 tgttgagccc gtcatatttg accgtgccct tggcccgcat cgcgttcctg gcctgctcaa
     6841 ccgcggtgct catttcgtcc ttggccttgg tgaaatcgac caccgtcacg gtatatgtgc
     6901 cgctcagccg cgagccgggc ggcgcgatag cggtgaagat gttggcggtg agagtggtgc
     6961 ccttggccgt cttgtagggc gcttgcgtca tcaccgggtc gcccggcaga ttgacgctga
     7021 agaaattctc gcgattggcg taagtgtccc aggactgcgc ccaggcggca cccgaccagc
     7081 ctgcacttga aaagaaaaca accgcgagcg cgggaataag gccggcaaag cgcatgggga
     7141 caccctcttg gtccagataa gacggacatt gccacaaaat gcccggtcaa tgcacctccc
     7201 gtacccgcga cccgcgcggc gtcattcccg gggcgcgggc gcggccgcgc acccttgcgc
     7261 acgggccagg agaaccgcac gttcgcgtgt gttccgcgtg aggctcgcgg cgtgttcgaa
     7321 tgcggcacgc gcttcggcgt tgcggccgag tttcgccagg agatcgccgc gcacggcagg
     7381 caggagataa taggtcttga gggtgcgttc gccgttcagc gcatcggcga tttcaagccc
     7441 ggccgccgga ccgaacgcca tgcccgccgc caccgcgcga ttgagttcca cgacaggcga
     7501 cggcgcgcat tcggagagcg cgccgtacag cgccgcgatg cgcgcccagt cggtctcatc
     7561 ggcgcttcgt gcccgcgcgt ggtaggcggc gatcgcggcc tgcagcgcat aaggcccaag
     7621 cgcgccgcca agcccttcgg cgcgggccag cgccgcaaga ccgcgccgga tcagaacctg
     7681 gtcccagcgg gcgcggtttt gatccagcag caaaacgggc gcgccatccg cgccggtccg
     7741 cgcccgggag cgtgattcct ggatctccat cagcgcgacg aggccatgga cctccggctc
     7801 gcgcggcgcc agttccgcca ggatgcggcc cagacgcagc gcgtcctcgc agagttgcgg
     7861 acgcatccaa tcctcgccgg tggttgccgc gtagccctcg ttgaagatga gatagatcgc
     7921 ctccagcacc gatgacagac gcggcgccag ctcgggtccg cggggcacct cgaatgccac
     7981 agccttttcc gcgagggtgc gtttggcccg tacgatgcgc tgggcgatgg tgggctccgg
     8041 caccaggaac gcgcgcgcga tctcctcggt ggtcaacccc ccgatcagcc gcagcgtcag
     8101 cgccacccgc gcctcggtcg aaagcaccgg atgacaggcc gtgaacatca ggcgcaggag
     8161 atcatcgccg atatcgtcat cgagagcggc atcgagatcc ggcacggccg tctcctgctc
     8221 gatctccaat tcgcggccca gctcctcatg cttgcgcgcg agcaatttct tccgccgcag
     8281 ccggtctatc gcgcgatgct ttgccgtcgc catcagccat gcgcccggat tttcgggaat
     8341 gccattttgc ggccatttct cgagcgcgat gacgagggca tcctgagcaa gctcctcggc
     8401 gagcccgaca tcgcgcacaa tccgagcgag gccggcgata agccgcgcct gctcgatcct
     8461 ccagatcgcg tcgatcgtgc ggtgggtgtc gttggacgtc acccgctacc gattagacat
     8521 cctcactttt gcgcgcaagc gtcaggaaac tcaggagacg tcctcaaagc aggttcccac
     8581 ctcgcgcacc tcgacagtcg cccatttcgc cgccgggcac gcttgcgcga tggcgatggc
     8641 ttcctcccgg gtcgcgcagt cgagcaggaa gaaaccgccg accatttcct tggattccgc
     8701 gaacgggcca tcgacgat
//
LOCUS       DASYEG010000253         6838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1249680, whole genome shotgun sequence.
ACCESSION   DASYEG010000253 DASYEG010000000
VERSION     DASYEG010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6838
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249680"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="VGM72_10910"
     CDS             complement(<1..1289)
                     /locus_tag="VGM72_10910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY4265820.1"
                     /translation="MRKFAFAGVGVAVGFVSALALFPAAQGATNVLSAYRELDLFGDA
                     FERVRANYVREVDDSELINSAINGMVSSLDPHSSYMDPKEFADMQVQTRGEFGGLGLE
                     VTQEDGLVKVVTPIDDTPASRAGVKTGDFISAIDGTSIQGMPLNEAVERMRGPENSKV
                     TLTILRQGEKKPLELTLTRAVIRVESVKYEKKGDVGYIRITSFSEKTDAGLKKAMADL
                     KKQLGGSLRGWVLDLRNDPGGLLDQAIAVSDDFLTGGEVVSTRGRKPEDTQRYNARAG
                     DLADGKPVVVLINEGTASASEIVAGALQDHKRATIIGVTSFGKGSVQTIIPLGGQHGG
                     ALRLTTARYYTPSGRSIQATGITPDIAVSNLTEKDQSEADQFSARTEASLAGHLDAEG
                     AQRNTKMPTVRPEDGKKIDDFQLSYALDRIDGKVVSAA"
     gene            complement(1286..2512)
                     /locus_tag="VGM72_10915"
     CDS             complement(1286..2512)
                     /locus_tag="VGM72_10915"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY4265821.1"
                     /translation="MNGRPLSVGLFVLMVSAAAVSVASHAAAPARGGPPPSGATLAER
                     YARAQKAFEEQRDSEDKTRTERDALAAEARALQERLVGNASKVQDLEAAVEGTTDELD
                     RLNETVRTLQSDLLRDRDRVAHLLAVLQRLQTDEPPALALKPGDSLAAARGTMQMGAM
                     LPPVYQEAAALAKKLKTLGETRIALAKKGAEARVEADSLKTARASLDQLLQEKSTEQT
                     DADAKLSEIHEITEEIGREASGLKALIDRVASLRAAAPASEGMKIVTAQNGTVGALSR
                     GSLLRPVVGSATPGDPAGPGRTPGENGPLGLWFETAGRAEAVAPADSEVVFAGTYQKF
                     GQVLILEIAGGYHLTLAGLGRIDVHIGDSVLAGEPVGVLPEGRAARLYMELRRNGQAV
                     DPAPWLSAELRKAKGT"
     gene            complement(2509..4110)
                     /gene="gpmI"
                     /locus_tag="VGM72_10920"
     CDS             complement(2509..4110)
                     /gene="gpmI"
                     /locus_tag="VGM72_10920"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEY4265822.1"
                     /translation="MNDSPSSSPPGNNRQRPAVLCVLDGWGWRPNGADNAIALARPAN
                     FERMMRECPYALLATSGRAVGLPEGQMGNSEVGHTNIGAGRLVMQDLVRIQDAVADGS
                     LAKRPDLQALIDAAKRATATVHLMGLMSPGGVHSHQDHMAALTRILGSVGLRVRVHAF
                     LDGRDTPPQSALGFVGSFLQAIANVPNAAIATLGGRYYAMDRDKRWDRVAKAYACIVD
                     AKGPRFSDAKTAIEKSYAGGVNDEFVLPCVLGDYAGIADGDALLFANFRPDRAREISA
                     ALLDPGFDGFARATTPRFSAAAGMTQYSEPLARFMSALFAPQDVSETVGEIFAERGLK
                     QLRIAETEKYPHVTFFMNGGREAQFEGEDRIMVPSPKIATYDLKPEMSAYDVTDKLVA
                     AIGSGKYDLIIANYANPDMVGHTGMMDAAIAAVKVVDSCLGRLRDAVEKAGGVLIVTA
                     DHGNVEQMLDPVTGAPHTAHTLLDVPIIVVNAQTTGSRLHLRNGRLADVVPTLLDLVG
                     FAKPPQMSGESLLSRETASTAGAQA"
     gene            4290..4982
                     /locus_tag="VGM72_10925"
     CDS             4290..4982
                     /locus_tag="VGM72_10925"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY4265823.1"
                     /translation="MRTHIGTVALSAISFLVFVPGLVFVPGADAEQAHSNRALSAPMP
                     ALAAAPLYAAPKADAGLVPAGSYRLDRGHASVTAQIRREGLSNFTFRFDRFDASFSYD
                     PKKPAQTLLLVTLDPASVDANVPAWTEHLQAADLLDTAKFLQISFVSTNFLQTGPATG
                     VLVGNVTFLGVTKPMAVEVTLNGVASGPRTVVGFSGRAAIKMADFPMPGFAALHLGND
                     VQFGIEAEFIKS"
     gene            complement(4972..5145)
                     /locus_tag="VGM72_10930"
     CDS             complement(4972..5145)
                     /locus_tag="VGM72_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265824.1"
                     /translation="MAISGILPRSPMPIPCRAALKKMSVKSDSYKSNIGDQAGFGQNM
                     PGASAMPGKKLSF"
     gene            5144..6265
                     /locus_tag="VGM72_10935"
     CDS             5144..6265
                     /locus_tag="VGM72_10935"
                     /inference="COORDINATES: protein motif:HMM:NF014206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEY4265825.1"
                     /translation="MARLPTRFISTLSVLALAFAPGPAASFSRIKDLADVEGIRDNML
                     IGYGLVVGLNTSGDSLKNAPFTQQALQTMLERLGVNTRGAAINTKNVAAVMVTANLGP
                     FSAQGTHMDVTVSAMGDAKSLQGGSLLATPLLAADGQVYALAQGPVAIGGFSAQGAAS
                     SVTRGVPTSGRISNGAIVEREVGYSFAKAQSLRLSLRNPDLTTAQRVASAINRYLGVP
                     AASATNPATIQLLKPASYTDVVALLTDIEQLKVEPDLPAKVVIDEQSGVIVMGSDVRI
                     STVAIAQGNLTIRITETPQVSQPQPFSMGGQTQVVPRTNIDVDEEAGKRLVVLNAGIG
                     LQELVNGLNALGIGPRDMISILQAIKAAGALQAEIQVIG"
     gene            6262..6588
                     /locus_tag="VGM72_10940"
     CDS             6262..6588
                     /locus_tag="VGM72_10940"
                     /inference="COORDINATES: protein motif:HMM:NF021638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HEY4265826.1"
                     /translation="MSDLGATGDLQLSSALSQSKPALPPLSAKNADQAGKVAKDFEAV
                     FINEIMGAMFEGIKTDGPFGGGPGEGIFRSMMIEHYSKTIADQGGFGLATAVKRELLR
                     AQEKAS"
     gene            6585..>6838
                     /locus_tag="VGM72_10945"
     CDS             6585..>6838
                     /locus_tag="VGM72_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265827.1"
                     /translation="MTAPANIDAGVLTEERQRMRLEQLLTVSRRLTAAIGEDIAALQT
                     GAFDALRTTDPEIERLCAFYGREVRALKAEGGVKRAPAALL"
BASE COUNT         1234 a         2243 c         2054 g         1307 t
ORIGIN      
        1 gccgccgaga ccacttttcc gtcgatccgg tccagcgcat aggagagctg gaagtcgtcg
       61 atcttcttgc cgtcttccgg ccggacggtc ggcattttgg tgttgcgttg ggcgccttcg
      121 gcgtccagat ggcccgcgag agaggcttca gtgcgcgcgc tgaactgatc ggcctcggac
      181 tgatccttct cggtcagatt cgagaccgcg atgtcgggcg tgatgccggt cgcctgaatg
      241 gagcggcccg acggcgtgta atagcgcgcc gtcgtcagac gcagggctcc gccatgctga
      301 ccgccgagcg ggatgatcgt ctgcaccgag cccttgccga acgaggtcac gccgatgatg
      361 gtcgcgcgct tgtggtcctg cagcgcgcca gccacgattt ccgacgccga ggccgtgcct
      421 tcattgatca gcacgacaac cggcttgcca tccgcgagat cgcccgcgcg cgcattgtag
      481 cgctgcgtat cctcgggctt gcggccgcgg gtggacacca cttcgccgcc ggtgaggaaa
      541 tcgtcggaca ccgcaatcgc ctgatccagc agaccgcccg gatcgttgcg caaatccaga
      601 acccagccgc gcaaagagcc gccgagctgc ttcttcagat cggccatcgc cttcttgagg
      661 cccgcatccg tcttctcgct gaaagaagtg atgcggatat atcccacatc gcctttcttt
      721 tcgtatttca cgctttccac gcggatcacg gcgcgcgtga gcgtcaactc aagcggcttt
      781 ttctcgccct ggcgcaggat ggtcagcgtg actttggaat tctccgggcc gcgcatgcgc
      841 tccaccgcct cgttcagcgg catgccctga atgctggtgc cgtcgatggc cgagatgaag
      901 tcgccggtct tcacacccgc gcgcgaggcc ggcgtgtcgt cgatcggcgt caccactttg
      961 acgaggccgt cttcctgcgt gacttcaagg ccgagaccgc cgaactcgcc gcgcgtctgc
     1021 acctgcatgt cggcaaattc tttcgggtcc atatagctcg aatgcggatc gagcgagctg
     1081 accatgccgt tgatcgcgga gttgatgagt tctgaatcgt cgacttcgcg cacataattg
     1141 gcgcgcacgc gttcgaaggc atcgccgaaa agatcgagtt cgcgataggc ggacaacaca
     1201 ttcgttgctc cctgggcggc ggggaacaaa gccaatgcac tcacaaatcc aaccgccacg
     1261 ccgactccgg cgaatgcgaa ctttctcatg ttccctttgc ctttctcagt tcggcgctca
     1321 accacggcgc cggatcaaca gcctgaccgt tgcgtctcag ctccatgtag agccgtgccg
     1381 ctctgccttc cgggagaacg cctaccggtt cgccagcgag cacggaatct ccgatgtgaa
     1441 catcgatgcg accgagcccc gcaagcgtca gatggtaacc gccagcgatt tccaatatca
     1501 agacttgacc aaatttttgg taagtgcctg caaacacaac ttcgctatcc gccggtgcga
     1561 cagcttcggc tcggccggcc gtctcgaacc acaggcctaa cgggccgttt tcacccggag
     1621 tccggcccgg accggcagga tcgccagggg tcgcgcttcc cacgacaggg cgaagcagcg
     1681 agccgcggga aagcgcgccc actgtcccat tttgggccgt gacgatcttc atgccttcgg
     1741 acgcaggcgc cgccgcccgc aagctcgcga cccggtcgat caacgctttc aaaccgctgg
     1801 cttcccggcc gatctcctcg gtaatttcgt gaatctccga aagcttggca tccgcgtccg
     1861 tttgttcggt gcttttttcc tgcagcagtt gatcgaggga ggctctcgcg gttttcagac
     1921 tatcggcctc gacgcgggct tcagcgcctt tcttggccaa ggcaatacgg gtttctccca
     1981 gcgttttcag cttcttcgcg agagcggcgg cctcttggta aaccggcggc aacatggcgc
     2041 ccatctgcat ggtcccgcgc gccgcggcca gggaatcccc cggtttgaga gccagcgcgg
     2101 gcggctcatc cgtctggagc cgctgcagca cggcgaggag atgggcgact ctgtcacgat
     2161 cgcgcaacaa atcggactga agggttcgga ccgtctcgtt gagccggtca agctcatcgg
     2221 tggttccctc gacggcggct tccaggtcct gcaccttcga agcattgccg acgaggcgtt
     2281 cctgaagcgc gcgggcttcg gccgccagcg cgtcgcgctc ggtgcgggtc ttgtcctcgc
     2341 tgtcgcgctg ttcctcgaac gctttttgcg cgcgggcata acgctccgcc agcgtggccc
     2401 ccgagggcgg gggcccgccg cgcgccggag ccgccgcgtg ggacgcaacc gaaaccgccg
     2461 ccgccgaaac catgagcacg aaaaggccaa ccgaaagagg ccgcccattc atgcttgcgc
     2521 acccgccgtg cttgcggttt cgcggctgag aagcgattcg ccgctcatct gcggcggctt
     2581 cgcaaaaccc accagatcga gcagtgtcgg aacgacgtcg gcgagacggc cgttgcgcag
     2641 atgcaatcgc gaaccggtgg tttgggcatt gaccacgatg atcggcacat ccagcagcgt
     2701 gtgggcggtg tgcggcgcgc cggtcacggg atccagcatc tgctcgacat tgccatgatc
     2761 ggcggtgacg atcagcaccc cgcccgcttt ttcaacggca tcgcgcaggc gtcccagaca
     2821 ggaatccacg actttcaccg ccgcaatcgc cgcatccatc atgccggtat gtccgaccat
     2881 atcgggattg gcgtaattgg cgatgatgag atcgtatttt cccgatccga tggcggcgac
     2941 cagtttgtcg gtgacgtcat aggcggacat ttcgggcttc aaatcataag tggcgatctt
     3001 cggactcggc accatgattc ggtcctcgcc ttcgaactgc gcttcgcgtc cgccattcat
     3061 gaagaacgtc acatgcggat atttttccgt ctccgcgatg cgcaattgct tgagcccgcg
     3121 ttcggcgaaa atctctccaa cggtttcgct gacgtcctgc ggcgcgaaca gcgcgctcat
     3181 gaaccgggca agcggctcgg aatattgcgt catgccggcc gccgccgaga aacgtggcgt
     3241 cgttgcgcgt gcgaacccat cgaacccggg atccagcaga gcggccgaaa tctcgcgcgc
     3301 ccggtcggga cggaaattgg cgaacaacaa cgcatcgcca tccgcgatac ccgcataatc
     3361 gcccagtacg caaggcagca cgaattcgtc gttgacgccg ccggcatagg atttctcaat
     3421 cgccgttttc gcatcggaaa aacgcgggcc cttggcatcg acgatgcagg cataggcttt
     3481 cgccactcgg tcccagcgtt tgtcgcggtc catggcgtag tagcgcccgc ccagcgtcgc
     3541 aatcgccgcg ttcggcacat tcgcgatcgc ttgcagaaac gaacccacga aaccgagcgc
     3601 actttgcggc ggcgtatcgc gcccatccaa aaacgcgtgc acccggaccc tcaagccgac
     3661 gctgccgaga atgcgcgtta gcgccgccat atgatcctga tgcgaatgca cgccgccggg
     3721 cgacatcagc cccatcagat gcaccgttgc cgtggcgcgt tttgccgcgt cgatgagcgc
     3781 ttgcaagtcg gggcgtttcg ccagcgaacc gtccgcgacc gcgtcctgga tgcgcaccag
     3841 atcctgcatc accagacggc ccgcgccgat attggtgtga ccgacttcgg aatttcccat
     3901 ctgcccctcg ggcaggccga ccgcgcggcc cgatgtcgcc agaagcgcat acgggcattc
     3961 gcgcatcatg cgttcgaaat tggcgggccg cgcaagagcg atcgcgttgt cggcaccgtt
     4021 gggacgccag ccccagccgt cgagcacgca cagcaccgcc gggcgctgcc gattgtttcc
     4081 gggaggagac gaagacggag aatcgttcat gcggatctat cgatcaatgc gttgaatcgt
     4141 cgaggcgagt cctttcccgc aaaaataggt acacggctcc ccgctgtcca ttgcgggggg
     4201 cgaagcctgc gcgaaacccg ccgcttggtt cgccgcgctt ttgcgcataa aagcttttcg
     4261 catttcgtta aggcgccgga ggcattttct tgagaaccca catcggcacg gtggcactga
     4321 gcgcaatctc atttctcgta tttgttcctg gcctcgtgtt tgttccgggt gcagacgcgg
     4381 agcaggccca ttccaaccgg gccctctctg ccccgatgcc cgccctggcc gcagccccgc
     4441 tctatgcggc accgaaggcc gatgccggac ttgttccggc cggcagctat cggctggacc
     4501 gtggccatgc gagcgtgacg gcgcaaatcc gccgcgaggg gctgtccaat ttcaccttcc
     4561 gcttcgaccg tttcgatgcg agcttcagct atgacccgaa aaaaccggcc cagaccctgc
     4621 tgctggtcac gctcgatccg gcttcggtgg acgccaatgt tccggcctgg accgagcatc
     4681 tgcaggcggc cgatctgctc gacaccgcca aattcctcca gatctcgttc gtttccacca
     4741 atttcctgca aaccggcccg gccaccggcg tgctggtcgg taatgtcacc ttcctgggcg
     4801 tcaccaagcc gatggctgtc gaggtgacgc tgaacggcgt tgcgtcgggt ccgcgcacgg
     4861 tggtgggctt ttcgggacgc gcggcgatca agatggcgga tttccccatg ccgggtttcg
     4921 cggccttgca tctgggcaac gatgtgcaat tcggcatcga ggcggagttc atcaaaagct
     4981 gagcttcttg ccgggcatcg ccgaagcgcc cggcatattc tgcccgaagc cagcctgatc
     5041 tccgatattg gatttataac tatctgattt tacagacatt ttcttcaagg cagcgcggca
     5101 tggaattggc atagggcttc tcggaagaat tccggaaatt gccatggccc gcctcccgac
     5161 ccgctttatc tccactctat ccgtgctcgc actggcgttc gcgcccggac ccgccgcgtc
     5221 cttttcgcgg atcaaggacc tcgccgatgt cgaaggcatt cgcgacaata tgctgatcgg
     5281 ttacgggctc gttgttgggc tcaatacctc cggagattcg ctcaagaacg cgcccttcac
     5341 ccagcaggcc ctgcagacca tgctcgaacg gctgggcgtc aacacacgcg gtgcggcgat
     5401 caacaccaag aacgtcgccg ccgtcatggt gaccgccaat ctgggcccgt tctcggccca
     5461 gggcacccat atggacgtga ccgtttcggc catgggcgac gccaagagcc tgcaaggcgg
     5521 ttcattgctc gcgaccccgt tgcttgccgc cgatggacag gtctacgcac tggcgcaggg
     5581 gcccgtcgcg atcggcggct tctccgcgca aggcgcggcc tcgagcgtga cacgtggcgt
     5641 tcccacatcg gggcgcatct ccaacggcgc cattgtcgaa cgcgaagtcg gctattcctt
     5701 cgccaaggcg caatccctgc gtctctcgct gcgcaatccc gacctgacca ccgcacagcg
     5761 cgtggcgagc gcgatcaacc gctatctcgg cgtgccggcc gcgagcgcga ccaatccggc
     5821 cactatccag ctcctcaagc ccgcctctta tacggatgtg gtggcgcttc tgaccgacat
     5881 cgagcagttg aaggtcgaac ccgatctgcc cgcgaaagtc gtgatcgacg aacagtcggg
     5941 cgtcatcgtc atgggatcgg acgtgcgcat ctccaccgtc gccatcgcgc aaggcaacct
     6001 caccatccgc attaccgaaa cgccgcaggt gagccagccg cagcctttct cgatgggcgg
     6061 ccaaacccag gtcgtgccgc gcaccaacat cgatgtggac gaggaagcgg gcaagcgcct
     6121 ggtggtcctg aacgcgggca tcggcctgca ggaactggtg aacggtctga acgcgctcgg
     6181 catcggcccg cgcgacatga tctcgatcct gcaggcgatc aaggcggcgg gcgctctgca
     6241 agccgaaatc caggtgatcg gatgagcgat ctcggcgcca ccggcgatct gcagctttct
     6301 tcggcacttt cgcagtccaa accggctctg ccgcccctgt cggcaaagaa cgcggatcag
     6361 gcgggtaagg tcgcgaagga tttcgaggcg gtcttcatca acgagatcat gggcgcgatg
     6421 ttcgaaggca tcaagaccga cggaccgttc ggcggcggac cgggagaagg catcttccgc
     6481 tccatgatga tcgagcatta ttccaaaacc atcgccgatc agggtgggtt tggccttgcc
     6541 accgcagtga agcgcgaatt gttgcgagcc caggagaaag cgtcatgacc gcacccgcaa
     6601 acatcgatgc cggcgtcctc acggaggaac gtcaacgcat gcgcctggag caattgctga
     6661 cggtaagccg ccgtttgacg gcagcgatcg gcgaggacat cgcggcgttg caaaccggcg
     6721 ccttcgatgc cttgagaacc acggacccgg agatcgaacg gctctgcgct ttttatggac
     6781 gcgaagtcag ggccctgaag gccgagggcg gcgtcaaacg cgcgcccgcc gcgctgct
//
LOCUS       DASYEG010000254         5864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_5166715, whole genome shotgun sequence.
ACCESSION   DASYEG010000254 DASYEG010000000
VERSION     DASYEG010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5166715"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..611
                     /locus_tag="VGM72_10950"
     CDS             234..611
                     /locus_tag="VGM72_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265828.1"
                     /translation="MPKSESTAIKWLKKVEDQDYPAAKSYLLLLYDGKRVAKILKQLR
                     KVPICQFKAKDLFRASQLSVLGVSNSHIEKDRKKIRNGHGLSPLLLVRDAIHSRLIIA
                     DGYHRMCAVYGFDEDAWIHGKIV"
     gene            complement(663..737)
                     /locus_tag="VGM72_10955"
     tRNA            complement(663..737)
                     /locus_tag="VGM72_10955"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(703..705),aa:Gln,seq:ttg)
     gene            complement(929..1228)
                     /locus_tag="VGM72_10960"
     CDS             complement(929..1228)
                     /locus_tag="VGM72_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265829.1"
                     /translation="MTVNRRELIERRLACRHALTIPDLPQDIADGFKWFYRHADERLT
                     NERDPARARFCRECAAQWHMLAASARHAESREACLQTAVAWERLVLETHGDEAAA"
     gene            1442..1518
                     /locus_tag="VGM72_10965"
     tRNA            1442..1518
                     /locus_tag="VGM72_10965"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1476..1478,aa:Pro,seq:cgg)
     gene            1606..2505
                     /locus_tag="VGM72_10970"
     CDS             1606..2505
                     /locus_tag="VGM72_10970"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4265830.1"
                     /translation="MAIPFDTDFEAPYERLISASPRIRRMLTNNPGPFTFKGTGVAVV
                     GEGRVAVIDPGPDDPVHLERLRAALKPETVTHILVTHTHRDHSPAARALKQWTGAPTY
                     GFGPHGAGRADLGIAVEEGGDRDFVPDIAVRDGDIIEGAGFTLQCVHTPGHTSNHMCY
                     ALLEEKALFSGDHVMAWSTTVVAPPDGDMGDYLASLEKLLVRDDAIYWPTHGGPVRDP
                     KNFVAAYLAHRRARETQILAALDNGMTGVPQIVDRIYAGLDPRLKPAAALSVLAHLLH
                     LVKTGRAAASAGPPLTAHYRVTA"
     gene            2768..3379
                     /locus_tag="VGM72_10975"
     CDS             2768..3379
                     /locus_tag="VGM72_10975"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEY4265831.1"
                     /translation="MTESKPFTLAPLPYDEGALAPTISAKTMSFHYGKHHKAYVDKLN
                     GLVAGTKYAAMKLEEIVKATSGDSSQQEIFNNAGQAWNHAFFWNCLSPQGGRPAGGLM
                     RAVERDFGSYEAFQEEFAKKGVSQFGSGWVWLVADGDKLSIQKTPDAKSPMAEGKRCL
                     LTIDVWEHAYYLDYQNKRPDFLKAVVETLLNWDFAAQNFERAG"
     gene            3590..3844
                     /locus_tag="VGM72_10980"
     CDS             3590..3844
                     /locus_tag="VGM72_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265832.1"
                     /translation="MDQVTALIRRNVQVGVKSRSVSLIAFAIFVVALIVLIDLALNEE
                     NRNVRTMLYMLAAFSFVAVLGQVPILRSMITRKKNREGVR"
     gene            3841..5046
                     /locus_tag="VGM72_10985"
     CDS             3841..5046
                     /locus_tag="VGM72_10985"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY4265833.1"
                     /translation="MTDCIIFDAVRTPRGRGKKDGALHEVTALELATQALKAVAGRNA
                     IDTAELDDVIMGCVDPVGEQGADIARAAVLKAGFADTVPGMQINRFCASGLDACNMAA
                     GQIMSGQSDLAIGGGVEAMSRVAIGSSGGAWPEDPSIAIPAYFVPQGISADLIATLDG
                     FSRDDVDALAVESQKRAANAWAKGYFKHSVIPVKDAQGLTILDRDEHMRPDTTMQSLG
                     ALKPAFAEFGALGFDSVAIQRYPQIEALNHVHHAGNSSGIVDGAAAVLLGSRQKGTAL
                     GLKPRAKVKAFTAIGSEPAIMLTAPAEVTRKVLARAGMTAKDIDLFEINEAFASVVLH
                     FMRELDLSPDKVNVNGGAIAMGHPLGATGAMILGTVLDELERRNLATALVTLCIGAGM
                     GTATIIERI"
     gene            5055..>5864
                     /locus_tag="VGM72_10990"
     CDS             5055..>5864
                     /locus_tag="VGM72_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY4265834.1"
                     /translation="MNLVNFKWDQDQDGIVTLSWDTPGKSVNVLSMAAVAELAQVADA
                     IKTDAAIKGVVITSAKTGGFSAGADLDEMSAYAGHGGPDAAKSAFETMMNLHRAFRRI
                     ETCGKPVVAAINGTALGGGLELTLACHCRIAAGDPRIRLGLPEAQVGLIPGGGGTQRL
                     PRMIGAMAALPLMLEGRRIDPATAAGMNIVQKIVPAEELIPAAKAWIKANPGAQQPWD
                     KKEFRIPGGGPFSASGAQVFTVGNAMLRQKTYDNYPAQRFIMSAVYEGLLVD"
BASE COUNT         1128 a         1762 c         1839 g         1135 t
ORIGIN      
        1 gccagggacg ttgcggtccc ggaagccggt tcaaggagat ggcgtacgct ttgattaaat
       61 cccgtccgcg acaagcgacg cattcaagcg tggtcagcgc ccaagaagct tgactatccg
      121 cgccaccgat tatccgtatc atcacgctgt cacggatttg ccgatccggt aacgggccgc
      181 ccgcattggc gcggcggggt atgagcctct agggacaccg aggagaaaca actatgccta
      241 aatcagagtc gacagcaatc aagtggctca agaaagttga agatcaagac tacccggcgg
      301 cgaaatctta tttgctctta ctttacgatg ggaagcgtgt tgccaaaatt cttaaacagc
      361 ttcgcaaagt gccgatctgt cagtttaaag cgaaagacct gttccgagcc tcacaactgt
      421 cagtgctagg tgtcagtaac tcgcatatag agaaagaccg caagaaaatt aggaacggcc
      481 acggtctttc gccattgctt ttggttcgcg atgccattca cagcaggcta atcatcgccg
      541 atggttacca ccgcatgtgc gcagtttacg gatttgatga agacgcttgg atacatggca
      601 agatagtttg agtctcgtag gtcaaagacg atttgatcca agccgctaaa cgccgcgccg
      661 ggtggctggg gcggaaggaa tcgaaccttc gaatgccgga atcaaaatcc ggtgccttac
      721 cgcttggcta cgccccaatt tgccgaacca tatcgacttt tttgctgccg gcaatgcggg
      781 ctctacccga tttgcgaccc aacctgggat cacgctccga aagcgcccgc ttatagcaga
      841 ccggaacgca gcgtcgagcc ggtctgccgc gccggcctgc atggtttcat catctgcatg
      901 ttttcactga cccgcatcgc aatcgcgatc aggcagcggc ctcgtcccca tgcgtttcta
      961 agaccaggcg ttcccaggcg actgccgttt gaagacacgc ctctcgggat tcggcatggc
     1021 gtgcgcttgc ggcaagcatg tgccattgcg ccgcgcactc gcgacagaac cgcgcccgcg
     1081 ccggatcgcg ctcgttcgtg agacgttcgt ccgcatggcg gtagaaccat ttgaatccgt
     1141 ccgcgatgtc ctgcggcaga tcggggatcg tgagggcgtg ccggcatgca aggcggcgct
     1201 cgatcagttc tcggcggttg acggtcatga cttcggattc gaccccttgc agcgcgttca
     1261 ctctaacaga aaatgggcgc ccaaacgccc atgagcatga taatatcccg acggacgaac
     1321 cggcaagcgg taccgggcca tgccggctgg cgggaaggcg gacaaccgcc gcccggcgcg
     1381 ggatttttct ctcgcttgcc gcctcgggat cgcatcgcta taacgccgcc tctggccgcg
     1441 ccggagagta gctcagcctg gtagagcacc acgttcggga cgtgggggcc ggaggttcaa
     1501 atcctctctc tccgaccagt catttttgcg atgtttcgga tttgcggttt tgcgccccgc
     1561 aagtggcttg aagaatagcc gcgatgcggc gggcgagagc tgacgatggc gatcccgttc
     1621 gacacggatt tcgaagcgcc ctatgaacgc ctgatctcgg ccagcccgcg tattcgcagg
     1681 atgctcacca acaatccggg tcccttcacc ttcaagggca ccggcgtggc ggttgtggga
     1741 gaagggcggg tcgcggtgat cgaccccggt cccgacgatc cggtgcatct ggaacgcttg
     1801 cgcgcggcgc tgaagcccga gaccgtgacg catattctcg tcacccacac gcatcgcgat
     1861 cattcgcccg ccgcgcgcgc gctcaagcaa tggaccggcg cgccgaccta tggcttcgga
     1921 ccccatggcg cgggtcgcgc ggacctgggg atcgcggtgg aggagggcgg cgaccgcgac
     1981 ttcgtgccgg acatcgccgt gcgtgacggg gacatcatcg aaggggcggg gttcacgctg
     2041 caatgcgtcc acacgcccgg ccatacctcg aaccatatgt gttatgcgct tctcgaagag
     2101 aaggcgctat tttcgggcga tcacgtgatg gcatggtcga ccacggtggt ggccccgccg
     2161 gatggcgata tgggggacta tctcgccagt ctggaaaagc tgctcgtccg cgacgatgcg
     2221 atctattggc cgacacatgg cggcccggtg cgcgatccca aaaatttcgt tgccgcctat
     2281 ctggcgcatc gccgggcgcg tgaaacgcaa atccttgccg cgctggacaa cggcatgacg
     2341 ggggttcctc agatcgtcga ccggatttat gccgggctcg atccccggtt gaagcccgcc
     2401 gccgcactct ccgtgctcgc gcatctcctg catctggtca aaaccgggcg ggcggccgca
     2461 tccgccggcc cgcctttgac cgcgcattat cgcgttacgg cctgagtgcg cgccccaagg
     2521 gatcgaccgc gcccgggagc cgttgcgatc cgcctggaca aagggccgca catcggagaa
     2581 ttcgcgaaag cttgcgatct tcacgccgcg aaccgtgaaa atatgaagca caaacatgtt
     2641 tttccattga cgcctgatgg caagccggaa caccacaggc ctccagcagg gaacgcgcgc
     2701 gcgcgcggtg cgttagaccg tggactctgc gtgttttttc cccgttagga gagttgcgat
     2761 gaataccatg accgagtcca agccatttac actcgctccc ctgccctatg acgagggtgc
     2821 tcttgctccc accatttccg ccaagacgat gagctttcat tacggcaagc atcacaaagc
     2881 ctatgtcgat aagctcaacg ggctcgtggc gggaaccaaa tatgccgcga tgaagctgga
     2941 ggagattgtg aaggcgacat cgggcgattc ctcccagcag gaaattttca acaatgccgg
     3001 ccaagcttgg aatcatgcgt tcttctggaa ttgtctgtct cctcagggcg gccggcctgc
     3061 gggcgggctc atgcgggccg tcgaacgcga tttcggaagt tacgaggcgt tccaggaaga
     3121 gttcgcaaaa aagggtgtgt cgcaattcgg cagcggatgg gtgtggctgg tggccgatgg
     3181 agacaagctc tcgatccaaa agacgcccga cgctaaaagc ccgatggccg aaggcaagcg
     3241 ctgcctgctc acgatcgatg tgtgggagca cgcctattat ctcgactacc agaacaaacg
     3301 gcccgacttc ctgaaggcgg tcgtcgagac attgctcaat tgggacttcg cggcccagaa
     3361 tttcgaacgc gcaggctagt tctgttgctt ttgcgtttgt tgagttcgcg ccgcccctga
     3421 cctagggttc gtcccgaacc aaggggcggg cgcgcgcgcg atgaagtgct tctaccatcc
     3481 ccaaatcgac gcggttgggt tgtgtaagca ttgccagcgc ggactctgcc cggcttgcgc
     3541 ctcggagcgc gacggaggcc tcgcctgcca tgatcgtcat gaaagtcagg tggaccaggt
     3601 cacagcgctc atccggcgta atgtccaggt gggcgtcaaa tcgcgttctg tcagccttat
     3661 tgccttcgcg atcttcgtgg tggctttgat cgtgctgatc gatctggctc tgaatgagga
     3721 aaaccggaat gtcagaacca tgctgtatat gttggcggca ttttcgttcg tcgccgtgct
     3781 cgggcaggtt ccgattctgc gcagtatgat cacccgcaaa aaaaaccggg agggagtgag
     3841 atgacagatt gcatcatctt cgatgcggtg cgtacgccgc gcggacgcgg caagaaggac
     3901 ggcgcgctgc atgaagtgac ggcgctggag cttgccacgc aagcgttgaa ggcggtcgcc
     3961 ggccgtaacg ctatcgacac cgccgagctc gacgacgtga tcatgggttg cgtcgatccg
     4021 gtgggcgagc agggtgcgga tatcgcgcgc gccgccgtgt tgaaggcggg cttcgccgat
     4081 accgtcccgg gcatgcagat caatcgtttc tgcgcgagcg ggctggatgc ctgcaacatg
     4141 gccgccggcc agatcatgtc gggacagtcg gatcttgcga tcggcggcgg ggtcgaggcg
     4201 atgagccgtg tggcgatcgg ttcgtccggc ggcgcctggc cggaagaccc gtccatcgcc
     4261 attcccgcct atttcgtgcc gcagggaatt tcggccgacc tgatcgcgac gctggacggt
     4321 ttttcgcgcg acgatgtcga tgccctggcg gtggaaagtc agaagcgcgc ggccaatgcc
     4381 tgggccaagg gttacttcaa gcactcggtc atccccgtca aggatgcgca ggggctgacc
     4441 attctggacc gtgacgaaca catgcggccc gacaccacca tgcaatcgct tggcgcattg
     4501 aagccggcct tcgcggagtt cggcgcgctg ggtttcgatt cggtcgcgat ccagcgctat
     4561 ccccagatcg aggcgctgaa ccatgtccac catgcgggca attcgtcggg cattgtcgat
     4621 ggcgcggctg cggttcttct gggttcgcgg caaaagggca cggccttggg cctgaagccg
     4681 cgggcgaagg tgaaagcctt cacagccatc ggctcggagc ccgcgatcat gctgacggca
     4741 cccgccgaag tgactcgcaa ggttctggcg cgcgcgggca tgacggcaaa ggacatcgac
     4801 cttttcgaga tcaatgaggc cttcgcctcc gtcgtgctgc acttcatgcg cgaattggac
     4861 ctctcgcccg acaaggtgaa tgtgaatggt ggcgccatcg ccatgggcca tccgctcggc
     4921 gccacgggtg ccatgatttt gggcacggtt ctggacgagc tggaacggcg caatctcgcc
     4981 accgcgcttg tgacgctttg catcggtgcg ggcatgggca ccgcgacaat catcgaacgg
     5041 atttgaggac cgccatgaac ctcgtcaatt tcaaatggga ccaggaccag gacggcatcg
     5101 tcacgctgtc ctgggatacg ccgggcaaga gcgtgaacgt cctctccatg gcggcggtcg
     5161 ccgaactggc tcaggtggcg gacgcgataa agacggacgc cgccatcaag ggtgtcgtca
     5221 tcacgagcgc gaagaccggc ggtttcagcg ccggcgccga tctggacgag atgagcgcct
     5281 atgccggcca tggcggcccc gacgcggcca agtccgcttt cgagacgatg atgaatctgc
     5341 accgcgcctt tcgcaggatc gagacctgcg gcaagccggt ggtggcggcg atcaacggca
     5401 ccgcgctcgg cggagggctg gagcttacgc tcgcgtgtca ttgccgcatc gccgccggcg
     5461 atccgcgcat ccgcctcggc cttcccgaag cgcaagtggg tctgattccc ggcggcggcg
     5521 ggacacagcg cctgccgcgc atgatcggcg cgatggcggc tttgcccctg atgctcgaag
     5581 gacggcgcat cgatccggcg acggccgccg gcatgaatat cgtccagaaa atcgtgccgg
     5641 cggaggagct tattcctgcc gccaaggcct ggatcaaggc caatcccggc gctcagcagc
     5701 cttgggacaa gaaagaattc cgcattccgg gcggcggccc cttttcagcc tccggtgcgc
     5761 aggtgttcac cgtcggcaat gccatgctgc gccagaagac ttacgacaat tacccggcgc
     5821 aacgcttcat catgtcggcg gtctatgagg ggctgctggt cgat
//
LOCUS       DASYEG010000255        12722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4588081, whole genome shotgun sequence.
ACCESSION   DASYEG010000255 DASYEG010000000
VERSION     DASYEG010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 12722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12722
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4588081"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VGM72_10995"
     CDS             complement(<1..275)
                     /locus_tag="VGM72_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265835.1"
                     /translation="MQQISTLANLFTTRRPLALALSFGVFLAGFVYFLCRSRGFFSDE
                     GSYCTIAQGILHGELPYRSYFNEKPPLQYFWTAAIMAISWPTMSGARL"
     gene            complement(470..865)
                     /locus_tag="VGM72_11000"
     CDS             complement(470..865)
                     /locus_tag="VGM72_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265836.1"
                     /translation="MAHRKDSGGVDMMAGDFLRDHRIDKPHIVDAAPLTRLVLQLAAA
                     VPGAPHTIGIGDDKTIAVCQHLEFAAGAFSRRPSVATPAMEHEDKRCTGFEIARLIDE
                     ERTLASADRALIARECAGGLGGVRRGRNR"
     gene            1081..1368
                     /locus_tag="VGM72_11005"
     CDS             1081..1368
                     /locus_tag="VGM72_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265837.1"
                     /translation="MSTDETMGYFAGRNGLSNAPETTRLAHKNNADKTWVEQTAWTAP
                     GKPSVVLYTIHGGGHVVPQPYWRFPRNVGAQTEDLDAPAAIWDFFMKETGR"
     gene            complement(1410..3284)
                     /locus_tag="VGM72_11010"
     CDS             complement(1410..3284)
                     /locus_tag="VGM72_11010"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4265838.1"
                     /translation="MAFRKIIGVGALLIAAISAVAEAQPAPGSSDTPDQQPQASELSA
                     LDMSLLNAFKSGDYQNAIKILRPLAEAGNPKAQVMMGRFYGTGIGVPRDDTQAAGWYR
                     LAAEQGDPDGMNSLGYKLDHAVGPGQNPAEAAKWYKQAADLGNLPATNNLGLLYESGR
                     GVPKDYAEARRLYELAAAKGHTPSMNNIGVMYASGLGVPHDYVMAFEWVSRAANLGRA
                     ESMASLAVFYRLGRGVPRNYGEALRWARMGADLGDPYSMIQLGLLDLNGNGVTADRDA
                     AFALFERSAATGDARGALFLAAMYRDGLGTPQDANEAIKWYRRAADEGNDAGEVGLGT
                     VYFVQRNFAEAVRWFQSAAEKGVAAGQVRLGLFYLIGVGVPKDPAIAAQWFARAAEQG
                     DPEGQDVFGESYELGRGVPTDAARARELYRLSAEQGFALAEAKYAYLLQHGVGGPVDL
                     AGAIKNYLLAAQNGIVSAAANLAIAYSFGQGVAKDDVKASEFALQAAMVGEPGSQDTI
                     GYRLEHGLGTAENVPEAVKWYLRAAAQGQPNAMHNLAGLYATGRGVERDNSRACAWIA
                     LAVRNYPKNAPQLGAARQILDRCFAVLGPEDVEKMNAFTTTFKPSPEPQQGGISILDF
                     "
     gene            complement(3350..4144)
                     /locus_tag="VGM72_11015"
     CDS             complement(3350..4144)
                     /locus_tag="VGM72_11015"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY4265839.1"
                     /translation="MPYLSPALNVMIAAARKAARALIRDFGELENLQISRKGPADFVT
                     TADSRTERVLIEELSRARPGYGFLVEESGAIEGKDKTHRFIIDPIDGTTNFLHGIPQF
                     SISIALEREGHIVSGLVFNPVTDEMYVAEKGHGAYLNDRRLRVAARQHLPDILVASGA
                     PFMGKEGREEFLAELNAVVGATAGMRRFGSAALDLAWVASGRFDAFWERNLKPWDIAA
                     GMLIVREAGGVVSDLDGRDRMLECGHLLASNDTLHRQLLDLLRAKP"
     gene            complement(4164..4730)
                     /gene="efp"
                     /locus_tag="VGM72_11020"
     CDS             complement(4164..4730)
                     /gene="efp"
                     /locus_tag="VGM72_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY4265840.1"
                     /translation="MGKVAGNEIRPGMIIELDGQLWSVIKTQAVKPGKGGAYNQVEFK
                     NVVTGGKLNERFRSAETVDEVQLERRDFQYLYASGDTLTFMDMQNYDQIEIEADFVGD
                     RAQFLQDGMKVVLQMHEGKPLGVSLPPHVVLEITEADPVTRGQTAASSYKSAILENGV
                     RTQVPPFIEKGTKVVVSTDDGSYVRRAE"
     gene            complement(4869..5513)
                     /gene="thiE"
                     /locus_tag="VGM72_11025"
     CDS             complement(4869..5513)
                     /gene="thiE"
                     /locus_tag="VGM72_11025"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEY4265841.1"
                     /translation="MSKTRCRLYLITPPRFETKSFADMLKRALDAGDVASVQLRLKDL
                     PDDDILRAGEILMPITQRAGAAFILNDRPDLAARLGADGVHIGQEDVSYAEARAAMGR
                     DRIVGVTCHNIRHLAIEAAEAGADYVAFGAFFPTATKEAQTRAEPELIQWWSEMMVVP
                     CVAIGGINVENCGPLIEAGADFLSVASGVWDHPQGPAAAVKAFNAQFKLSSRPA"
     gene            complement(5510..6532)
                     /locus_tag="VGM72_11030"
     CDS             complement(5510..6532)
                     /locus_tag="VGM72_11030"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF012496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEY4265842.1"
                     /translation="MNGDQLIKTAKAMVAPGKGLLAADESSGTIKKRFDVIGVTSTET
                     SRRDYREMLFGTQAAMRNNISGVILYDETIRQNAKDGTPLVKLITDAGSIPGIKVDKG
                     TKPLPFCPGETITEGLDGLGERLDEYYKLGARFAKWRAVIDIAAGIPTYTAINTNAHA
                     LARYAAICQEHNIVPIVEPEVLMDGDHDIDTCFSVTQKVLKTVFQDLYHQRVMLEAMV
                     LKPNMVISGQKAAKRAAPQEVAEKTLRVLKDCVPPAVAGIAFLSGGQADEEATAHLDA
                     MNKMGPLPWPLTFSYGRALQHEPQKTWAGKAENVAAAQAAFAHRAHMNGLAALGQWKR
                     EAEKAA"
     gene            complement(6554..7744)
                     /locus_tag="VGM72_11035"
     CDS             complement(6554..7744)
                     /locus_tag="VGM72_11035"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY4265843.1"
                     /translation="MVRTLDDLGDVRGKRVLVRLDLNVPMKAGQVADLTRIERQAPTV
                     RELSDKGARVILLSHFDRPNGKIVPEMSLKPVTLALADVLGRPVAFAEDCIGATAEDA
                     AAKLKDGDVLLLENTRFHRAEEKNEPEFIRALAKLGDIYVNDAFSAAHRAHASTEGLA
                     HLLPSAAGRSMEAELTHLAKALEHPERPVMAVVGGAKISTKIALLENLLGRVDVLVIG
                     GAMANTFLAAEGRGIGRSLAEKDQLDTAKRIIDKAKSSGATILLPIDVVVARDFQAHA
                     ANRIVGINDVDEDEMILDVGPRTIQAFGRALDKMKTLVWNGPFGAFELPPFDRGTNAA
                     AGAVAERTRDKKLLSVAGGGDTVAALAHAGVVDAFSYVSTAGGAFLEWLEGRTLPGVA
                     ALER"
     gene            complement(7750..8757)
                     /gene="gap"
                     /locus_tag="VGM72_11040"
     CDS             complement(7750..8757)
                     /gene="gap"
                     /locus_tag="VGM72_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY4265844.1"
                     /translation="MAVRVAINGFGRIGRLVLRCILETGRKDLQVVAINDLGPPETNA
                     HLLRYDSVHGRFPGEVKVSGDRMIVDGHDLKVTAIKDPGQLPHKELGVDIALECTGIF
                     TSKERASAHLSAGAKRVLISAPADGVDLTVVYGVNHQKLTRDHIVVSNASCTTNCLAP
                     VAKVLNDTVGIEHGFMTTVHSYTNDQPSLDQMHKDLYRARAAAVSMIPTSTGAAKAVG
                     LVLPELKGRLDGTAIRVPTPNVSVIDFKVLAKRKTSVDEINEAMKRASSQELKGILDI
                     VDAPLVSADFNHNPASSSFALDQTKVMDGSFIRVMSWYDNEWGFSNRMADTAVAMAKL
                     I"
     gene            complement(8761..10758)
                     /gene="tkt"
                     /locus_tag="VGM72_11045"
     CDS             complement(8761..10758)
                     /gene="tkt"
                     /locus_tag="VGM72_11045"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004812554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY4265845.1"
                     /translation="MGEKPTSALRTMANSIRALAMDAVEAAKSGHPGMPMGMADAASV
                     LFTRFLKFDASDPAWPDRDRFVLSAGHGSMLLYALLYLTGYPGISMTDIKRFRQFGSV
                     CAGHPEHGEIPGIEMTTGPLGQGLATAVGMALAERILNARFGDAIVDHKTYVIASDGD
                     LMEGISHEACSIAGHLKLNRLIVLYDDNHITIDGSTALSDSTDALMRFEAMGWAVSRV
                     DGMDGDAVAAALEAAQSSDRPVLIACRTIIGYGAPKRAGTSKAHGEALGAEEIAGARA
                     ALGWPYPPFEIPPDLLSVWRTAGARGSESRKAWESRLSASPAASRAAFEAALSGDIPP
                     QFAPAIAALKAKFTNEKPAIATRKSSELVLEEVNANIPSVIGGAADLTPSTLTKTKND
                     PEISPGNYAGRYIHYGIREHGMAAAMSGMALHGGIIPYSGTFFVFSDYCRPAIRLAAL
                     MKQRVIHVMTHDSIGLGEDGPTHQPVEHLAALRAMPNLLVLRPADAVETAEIWEIALT
                     EKTRPSVLALSRQNLATLRDQHTEENLSRRGAYVLRESDGPAKVTILATGSEVEIAVA
                     ARDILQAQRIGARVVSMPCWELFEEQDPAYRAKVLDSSTVKVAIEAASSFGWSRYIGG
                     SGAFVGIDHFGASAPYKELYKAFGITAERAAEAAVALLKGN"
     gene            10920..11186
                     /locus_tag="VGM72_11050"
     CDS             10920..11186
                     /locus_tag="VGM72_11050"
                     /inference="COORDINATES: protein motif:HMM:NF025122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4164 family protein"
                     /protein_id="HEY4265846.1"
                     /translation="MSKLDDALARFQRAVDLLEARADECVANAYETAAVVAESGQWKG
                     ERERLAARIASLEEESRTLAGLTEEVEGRLDGAITEIRAVLGRN"
     gene            11200..11559
                     /locus_tag="VGM72_11055"
     CDS             11200..11559
                     /locus_tag="VGM72_11055"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEY4265847.1"
                     /translation="MAAVNVMVNGQAYPVGCGEGEEAHVRELGQFIDKRVRDIAGSIG
                     QVGDTRLLLMAGLAIADELADARAKLRERESDLLALRSELSGAAESRQKSEDIAAAAL
                     SGAASRLEAIAARLAHA"
     gene            11572..11729
                     /gene="ssrS"
                     /locus_tag="VGM72_11060"
     ncRNA           11572..11729
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VGM72_11060"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            11772..12362
                     /locus_tag="VGM72_11065"
     CDS             11772..12362
                     /locus_tag="VGM72_11065"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEY4265848.1"
                     /translation="MNIAESQALAAAKEDVRKEALARRAVLQPGVAELSRRLAQVFVN
                     SVAIPPGAVVSAYFAIGDEADPQPLLDLLRARGHKIALPRVVGRGKPLDFHLVETGAE
                     LVRGGFGLSEPHRDWPKVDPDVLAVPLLAFDQAGYRVGYGAGFYDRTLQRLRSSKTII
                     AAGYAFSAQEFPNLPHDENDQRLDWMATEKGARSFA"
     gene            12469..>12722
                     /locus_tag="VGM72_11070"
     CDS             12469..>12722
                     /locus_tag="VGM72_11070"
                     /inference="COORDINATES: protein motif:HMM:NF024674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HEY4265849.1"
                     /translation="MRILFLGDIIGRPGRDVLAAELPRLRERLTIDFVIANGENAAGG
                     FGITRVIANELFATGVDVLTSGNHWMDQREILTFIDAEEH"
BASE COUNT         2349 a         3946 c         3918 g         2509 t
ORIGIN      
        1 agacgcgctc cggacattgt gggccaactg atcgccatga tcgccgccgt ccagaaatat
       61 tgcagcggtg gcttctcgtt gaaataggag cgatagggca attccccgtg caagatgccc
      121 tgggcgatgg tgcaatagga accttcgtcg ctgaagaacc cgcgcgaacg gcagaggaaa
      181 tacacgaagc ccgcgaggaa aacgccgaaa gacagagcaa gcgccaaggg cctgcgcgtg
      241 gtgaaaagat tggcgagtgt cgaaatttgt tgcaaaggaa ttgccggtat cctgctggaa
      301 cggcgtcgtt tatgcgcaaa ctggcgtcca aatgccaaag cccgcggctt gttatcctta
      361 aaacgaaacg cccccatgct ataagtttgg gcggtttcat tgatcgaagg cggcaatgag
      421 agcggtgaaa tgtttcgcat gcgcgggcct tgtcgcgctc atcggggcgc taccggttgc
      481 ggccgcgccg gactccccca agaccaccgg cacattcgcg cgcaatcagg gcacgatcgg
      541 cggacgccag cgttctttct tcgtctatga gccgcgcgat ctcaaacccg gtgcagcgct
      601 tgtcttcgtg ttccatggcg ggggtggcga cggaggggcg gcgcgagaag gcaccggcgg
      661 cgaattcgag atgctggcag accgctatgg ttttgtcgtc gcctatcccg atggtgtggg
      721 gcgctcctgg aacggctgcc gcaagctgca gaacgagacg cgtgagcggc gcggcatcga
      781 cgatgtgggg tttgtcgatg cgatgatcgc gcaggaaatc gcccgccatc atgtcgaccc
      841 cgcccgaatc tttgcggtgg gccattccaa tggcggcgcg ctcgcctacc ggctcattct
      901 ggaacggccc aatgcattcg ctgcaatcgc ggcgatctcg tcgaacctgc ccgcgcccga
      961 caatatggaa tgtgccgcgc ttcccggtgc caagccggtg atggtcatca acggcacggc
     1021 cgatccggtg aatcctacaa tggcggcacc aatgggcgtg ggacgagcaa tggccgcacc
     1081 atgtcgaccg atgagaccat gggctatttt gccggccgca acgggctttc caatgcgccc
     1141 gagaccacgc ggctggccca caaaaacaac gccgacaaga cctgggtcga acaaacggcc
     1201 tggacggccc ccggcaaacc ttccgtcgtg ctttacacca tccatggcgg cggccatgtc
     1261 gtgccgcaac cctattggcg cttcccgcgc aatgtcggcg cacagaccga agatctggat
     1321 gccccggccg cgatctggga ttttttcatg aaggaaacgg gccgctaaga cgcagggtta
     1381 gggacgccaa ggcaccaatc gtacaaattt tagaaatcga ggattgaaat gccgccctgc
     1441 tgcggctctg gacttggctt gaaagttgtc gtgaacgcgt tcattttctc cacatcctcg
     1501 ggccccagca cagcaaaaca ccgatcgaga atctgacgcg ccgcgccaag ttgaggtgcg
     1561 ttcttgggat agttgcgaac cgccagcgcg atccaggcgc aggcacgaga attgtccctc
     1621 tcaacaccac gcccggtcgc atacaatccc gcaagattgt gcattgcgtt gggttggccc
     1681 tgggcggcgg cgcgcagata ccatttcacc gcctctggaa cattctcagc ggttccaagc
     1741 ccgtgctcaa gacgatagcc gatcgtgtcc tgcgagcccg gttctcccac catggcggcc
     1801 tgaagagcga attcacttgc ttttacatcg tccttggcaa cgccctgacc gaaactgtaa
     1861 gcgatcgcga gattggctgc cgccgataca atgccgttct gagcggccag gagataattc
     1921 ttgatcgcgc ccgcgagatc gacgggtcca cccacgccgt gctgaagcag gtacgcatat
     1981 ttcgcttcgg ccaacgcaaa gccttgttcg gcggagagac ggtacagttc gcgcgcccgt
     2041 gctgcgtccg tgggcacacc acgacccagt tcgtaggatt cgccgaacac atcctgcccc
     2101 tcgggatcgc cttgttcggc agcgcgggca aaccattggg ctgctatcgc gggatctttc
     2161 ggtacgccca ccccgatcaa atagaatagt cccaggcgca cttgccccgc ggccacgccc
     2221 ttttcggcgg cactttgaaa ccagcgcacg gcttcggcga agtttcgttg tacgaaatag
     2281 actgtgccga ggcctacttc tccggcgtca tttccttcat ctgccgcacg cctgtaccat
     2341 ttgatggctt cattggcgtc ttgcggggtc cccaaaccgt ctctgtacat ggcggccagg
     2401 aaaagcgctc ctcttgcgtc gcccgttgcc gcgcttcgtt cgaacaaggc gaatgccgca
     2461 tcgcgatcgg ccgttacgcc attcccattc aggtccagaa gtcccagctg gatcatcgaa
     2521 taggggtcac ccaaatcggc gcccatacgt gcccaacgca aagcctcacc atagttgcgc
     2581 ggcacgccgc gtcccaaacg ataaaagact gcaagcgagg ccatggattc cgcccggccg
     2641 agattcgccg cccggcttac ccattcgaac gccatgacgt aatcgtgcgg gactcccaat
     2701 cctgaggcgt acatcacgcc aatattgttc atggacggcg tatggccttt ggcggccgca
     2761 agttcataca gccttcgtgc ctcggcgtag tcttttggga cgccacggcc gctttcgtag
     2821 aggaggccca gattgttggt ggcgggtagg tttccgagat cggcagcctg tttataccac
     2881 ttcgcggctt ccgccggatt ctgccccggc ccgacggcgt gatcgagctt gtagcccaga
     2941 ctgttcatgc catccggatc gccttgttcg gccgcgaggc gataccagcc ggcggcctgg
     3001 gtgtcgtctc gtggcacccc gatccccgtt ccgtagaagc gccccatcat cacttgggcc
     3061 ttgggatttc ctgcttccgc caatgggcgc aggattttga tcgcgttttg atagtccccg
     3121 ctcttgaacg cgttcagcag cgacatatcg agtgccgaaa gctcggaagc ctgtggttgc
     3181 tgatcgggcg tatcggaact tccgggcgct ggttgcgcct ccgccaccgc agagatcgcg
     3241 gcgataagca aagcgccgac accgatgatc ttgcgaaaag ccaagccctg tctccccaaa
     3301 cagaccgtca gattgtcaca tgccttgaca ttattgtcac ggcttggcgt cagggtttgg
     3361 cgcgcagcaa atccaacaat tgacgatgca gcgtatcgtt cgaggccaaa agatggccgc
     3421 actccagcat gcggtcgcgc ccgtcgagat cggacaccac gccgcccgcc tcgcgtacga
     3481 tcagcatccc ggccgcgatg tcccagggct tcagattgcg ttcccagaac gcatcgaagc
     3541 ggcccgaagc gacccaggcc agatcgagcg ccgccgaacc gaagcgccgc atgcccgccg
     3601 tcgcgccgac aacggcgttc aattcggcga ggaattcttc gcgcccttcc ttgcccatga
     3661 agggcgcgcc cgacgcgacc aggatgtccg gcagatgttg gcgcgccgcg acccgcaagc
     3721 gccggtcgtt cagataggcg ccgtggcctt tttcggcgac atacatctcg tctgtcaccg
     3781 ggttgaacac gaggccggac acgatgtgtc cctcacgttc cagcgctatg gagatcgaga
     3841 actgcgggat gccgtgcagg aaattggtgg tgccgtcgat gggatcgatg atgaagcggt
     3901 gggtcttgtc cttgccttcg atggcgccgg attcctcgac caggaatccg tatccgggcc
     3961 gggcgcgcga aagctcctcg atcagcacgc gctcggtgcg cgaatcggcg gtcgtcacga
     4021 agtccgccgg gcctttgcgc gaaatctgca gattctccag ctctccgaaa tcgcggatca
     4081 acgcacgcgc cgccttgcgg gcggctgcga tcatcacatt cagggctggg gacagataag
     4141 gcactagctt cggttcgtcc ttatcattcc gcgcgccgga cgtagctgcc gtcatcggtg
     4201 gagacaacca ccttggtgcc tttttcgatg aagggcggca cctgggtccg cacgccgttt
     4261 tccaatatcg ccgatttgta ggacgaggcc gccgtctgcc ctctggtaac gggatcggct
     4321 tcggtgattt cgagcaccac atgcggcggc aggctgacgc cgagcggttt gccctcatgc
     4381 atttgcagga cgactttcat gccgtcctgc aggaattgcg cgcgatcgcc gacgaaatcc
     4441 gcttcgattt cgatctggtc gtaattctgc atgtccatga aggtcagcgt gtcgccgctg
     4501 gcatagagat attggaagtc gcgccgctcg agctggactt cgtccacggt ttccgccgaa
     4561 cggaagcgct cgttcagctt gccgccggtg acgacattct tgaactcgac ctgattatag
     4621 gccccacctt tgccgggttt gaccgcttgg gtcttgatca ccgaccagag ctggccatcc
     4681 agctcgatga tcatgccggg ccggatttca ttgcctgcga cttttcccat gatctcgtaa
     4741 cttttttaaa gagcgcgcgg ttctagcagt ggctatcgct cttgtcatcc cggaagccgc
     4801 gtgtacgcgg caatccggga ctcacgatgc aatggtctca gtggatcccg gctcgcgctt
     4861 ccacttcgct acgctggccg ggatgacagt ttaaattgtg cgttgaaggc tttcaccgcg
     4921 gcggcaggcc cttgcggatg atcccatacg ccggatgcca cgctcaagaa atccgcgccc
     4981 gcttcgatca gggggccgca attctccaca ttgatgccgc cgatggcgac gcacggcacc
     5041 accatcattt cagaccacca ttgaatgagc tcgggttcgg cgcgcgtctg cgcctccttt
     5101 gtcgcggtcg gaaaaaacgc tccgaaagcg acgtaatcgg cccccgcttc ggcggcctcg
     5161 atggcgaggt gccggatgtt gtggcaagtg acgccgacga tgcggtcgcg tcccattgcc
     5221 gcgcgggcct cggcatagga cacatcttcc tgaccgatat ggacgccgtc cgcgcccaag
     5281 cgcgcggcaa gatcgggccg gtcgttgagg atgaaggcgg caccggcacg ctgtgtgatg
     5341 ggcatcagga tctcgcctgc gcgcaggatg tcgtcgtcgg gcaaatcctt cagacgcaat
     5401 tgcacgctcg cgacatcgcc cgcgtccagc gcgcgcttca gcatatccgc gaaggatttt
     5461 gtttcgaatc tgggtggcgt gatcagataa agccggcagc gcgtcttgct cacgcggcct
     5521 tttccgcctc gcgcttccat tgccccagcg ccgccagccc gttcatatgg gcgcgatggg
     5581 cgaaggcggc ttgcgcggcc gccacattct ccgcctttcc ggcccaggtc ttctgcggct
     5641 catgctgcag cgcgcggccg taagagaatg tgagcggcca gggcagcggc cccatcttgt
     5701 tcatcgcatc gagatgcgcg gttgcttctt catcggcttg cccgccggaa aggaatgcga
     5761 ttcccgcgac cgccggcggc acgcaatcct tcagcacacg gagggttttc tccgcgactt
     5821 cctgcggggc cgcccgcttg gccgccttct gtcccgagat caccatgttc ggcttcagca
     5881 ccatggcttc cagcatcacg cgctgatgat agagatcctg gaaaaccgtc ttcagcacct
     5941 tctgggtaac gctgaagcag gtgtcgatgt cgtggtcgcc atccatcagc acttcgggct
     6001 cgacgatggg aacgatgttg tgttcttggc agatggcggc atagcgcgcg agcgcgtgtg
     6061 cattggtgtt aatcgcggtg taagtgggaa tgccggctgc gatgtcgatc accgcgcgcc
     6121 atttggcgaa gcgcgcaccg agcttgtaat attcgtccag gcgttcgccg agaccgtcca
     6181 agccttcggt gatggtttcg cctgggcaga agggaagggg cttggtgccc ttgtcgacct
     6241 tgatgccggg aatggagccg gcatcggtga tcagcttgac gagcggcgtg ccgtccttcg
     6301 cgttctggcg gatcgtttca tcataaagga tcacgccgga aatgttgttc ctcatcgcgg
     6361 cttgggtgcc gaacagcatc tcgcgataat cgcggcggct cgtttcggtc gaggtcacgc
     6421 cgatgacgtc gaaacgcttt ttgatcgtgc ctgagctttc gtcggcggca agcaggccct
     6481 taccgggtgc caccatcgcc ttggccgttt tgatcaattg atcgccattc atgatgtaac
     6541 cctcatttac gtctcagcgt tccaaagcgg caacgccggg aagcgtgcgt ccttcgagcc
     6601 attccaggaa cgcgccgccc gccgtcgaga catagctgaa cgcgtccacc acgcccgcat
     6661 gggccagggc cgctactgta tcgccccccc ccgcaaccga aagcagcttt ttgtcgcgcg
     6721 tccgctcggc caccgcaccc gccgccgcat tggtgccgcg gtcgaacggg ggcaattcga
     6781 aagcgccgaa cggcccgttc cagaccaggg tcttcatctt gtcgagggcg cgtccgaagg
     6841 cctggatggt ccgggggccg acatcgagga tcatctcgtc ctcatcgacg tcgttgatgc
     6901 cgacgatgcg gttggccgca tgggcctgaa aatcgcgcgc cacgacgacg tcgatgggaa
     6961 gcaggatcgt cgcgccagag gatttcgcct tgtcgatgat ccgtttggcc gtatcgagct
     7021 gatccttttc ggcaagcgag cgaccgatgc cgcgtccttc cgcggcaaga aatgtgttcg
     7081 ccatcgcgcc cccgatcacc agcacatcga cccgcccgag gagattttcg aggagcgcga
     7141 tcttggtgga aatcttggcg ccgccgacca ccgccatcac ggggcgttcg ggatgttcga
     7201 gcgccttggc gagatgggta agttccgcct ccatcgagcg ccccgcggcg gaggggagga
     7261 gatgcgccaa gccttcggtt gaggcgtgcg cgcgatgggc ggccgagaat gcgtcattga
     7321 catagatgtc gccgagcttc gccagcgccc ggatgaattc gggctcgttc ttttcctcag
     7381 ccctatggaa gcgcgtgttt tcgagtaaca gcacatcgcc gtctttcaat ttggcggcag
     7441 cgtcttcggc ggtggcaccg atgcaatcct ccgcaaacgc cacgggccgg ccgagcacgt
     7501 cggcgagcgc caaggtcacc ggcttcagcg acatctccgg cacgattttg ccgttcggcc
     7561 ggtcgaaatg cgaaagcagg atcacgcgcg cgcccttatc cgacagctcg cgcacggtcg
     7621 gcgcctgccg ctcgatgcgc gtgaggtctg cgacttggcc tgccttcatc ggaacgttca
     7681 gatcaaggcg caccaggacg cgcttgcccc tcacgtcccc cagatcgtcg agcgtgcgga
     7741 ccatggaccc tagataagct tggccatcgc cacggcggta tcggccatgc ggttggagaa
     7801 gccccactca ttatcgtacc aggacatgac ccggatgaaa ctgccgtcca tcactttggt
     7861 ctggtcgagc gcgaaagacg aggacgccgg attgtggttg aagtcggccg agacgagcgg
     7921 cgcgtccacg atgtccagaa tgcccttcag ctcctggctc gacgcacgct tcatggcctc
     7981 gttgatctcg tccacgctgg tcttgcgctt ggcgagaacc ttgaaatcga tcaccgacac
     8041 gttgggcgtg ggcacgcgaa tggccgtacc gtcgagccgg cccttcagct ccggcagcac
     8101 cagacccacc gccttggcgg cacccgtcga ggtcgggatc atggacaccg ctgccgcgcg
     8161 cgcgcggtag aggtccttgt gcatctggtc gagcgagggc tgatcgttgg tgtaggaatg
     8221 caccgtcgtc atgaagccgt gctcgatgcc gacggtgtcg ttcagcacct tggcgaccgg
     8281 cgcgagacaa ttggtcgtgc aggacgcatt cgacaccacg atgtgatcgc gcgtcagctt
     8341 ctgatgattg acgccataga ccaccgtcag gtcgacaccg tcggcggggg cggaaatcag
     8401 cacacgcttg gcgccggccg aaagatgcgc cgaggcgcgt tccttcgagg tgaagatgcc
     8461 ggtgcattcg agcgcgatgt ccacgcccaa ttctttgtgc ggcaattggc cggggtcctt
     8521 gatcgccgtc actttcagat cgtggccgtc cacgatcatg cggtcgcccg aaaccttcac
     8581 ctcaccggga aagcggccat gcaccgaatc gtagcgcaga agatgcgcat tggtctccgg
     8641 cggtcccaga tcgttgatgg cgacgacctg aaggtccttg cgccccgttt ccaagatgca
     8701 acgcagcacc aggcgcccga tacgcccgaa cccgttgatc gcaacccgca cggccatggt
     8761 tcaattcccc ttcagaagtg ccacggcagc ttctgccgcg cgctccgccg taatgccaaa
     8821 agccttgtaa agctccttat agggcgcgct ggcaccgaaa tgatcgatgc cgacgaacgc
     8881 gcccgagccg cctatatagc gtgaccagcc gaaagacgag gctgcctcga tagcaacctt
     8941 aacggtcgag gaatcgagaa ctttcgcgcg ataggcgggg tcctgttcct cgaacaactc
     9001 ccagcagggc atcgagacca cgcgcgcacc gattctctgg gcctgcagaa tgtcgcgggc
     9061 ggcgaccgca atctcgacct cggagccggt ggcgaggatc gtcactttgg cgggaccatc
     9121 gctttcccgg agcacatagg cgccgcgccg ggaaagattc tcctcggtgt gttggtcgcg
     9181 aagcgtggcg agattctgcc gcgatagcgc cagcacgctg ggccgtgtct tttccgtcag
     9241 cgcgatctcc cagatttccg cggtttccac cgcatcggcc gggcgtaata cgagaagatt
     9301 gggcatggcg cgcagggccg ccagatgctc gaccggctgg tgcgtcggtc cgtcttcgcc
     9361 gagcccgatg gaatcatgcg tcatcacatg gatgacgcgc tgcttcatca gggcggcgag
     9421 gcggatcgcg gggcggcaat aatcggagaa cacgaagaaa gtccccgaat aggggatgat
     9481 cccgccatgc agcgccatgc cgctcatggc cgccgccata ccgtgctcgc ggatcccgta
     9541 gtggatatag cgcccggcgt aattgcccgg agaaatctcc ggatcgttct tggtcttggt
     9601 cagcgtggaa ggcgtcaaat ccgccgcccc gccgataacc gacgggatat tggcgttcac
     9661 ctcttcgagc accaattcgg agcttttgcg tgtcgcaatg gcaggttttt cgttcgtgaa
     9721 tttggctttc agcgccgcaa tggcaggggc gaattgcggc gggatatcgc cggaaagcgc
     9781 ggcctcgaac gcggctcgcg acgccgccgg actcgcgctc aggcgcgatt cccacgcttt
     9841 gcgtgactca gagccgcgcg caccggccgt gcgccacacc gacaagagat cgggcgggat
     9901 ttcaaacggc ggatagggcc agccgagcgc ggcgcgtgcg ccggcgattt cttcggcccc
     9961 caaagcttcg ccatgcgctt tcgaggtccc tgcgcgtttg ggcgcgccat aaccgatgat
    10021 cgtccggcag gcgataagga cggggcggtc gctcgactgc gcggcctcga gcgcggccgc
    10081 gacggcgtct ccgtccatgc cgtcgacgcg cgataccgcc cagcccatcg cttcgaaacg
    10141 catcagcgcg tcggtggaat ccgaaagcgc ggtcgagccg tcgatggtga tatgattgtc
    10201 gtcatagagc acgatcagcc ggttgagctt cagatgaccg gcgatgctgc aggcctcgtg
    10261 gctgatgcct tccatcaaat cgccatcgct ggcgatgaca taggtcttgt gatcgacaat
    10321 ggcgtcgcca aagcgggcat tcaggatgcg ctcggccagc gccatgccca ccgcagtggc
    10381 aaggccttgc cccaacggtc ccgtcgtcat ctcgatgccg ggaatctcgc catgctcggg
    10441 gtgaccggcg cacacactgc cgaattgccg gaagcgcttg atgtccgtca tcgaaatacc
    10501 gggatagccg gtgagataaa gcagcgcata gagcagcatg gagccgtggc cggccgacag
    10561 cacgaaacgg tcgcggtcag gccaagccgg atcggacgca tcgaatttca ggaagcgggt
    10621 gaacaatacg ctcgccgcat cggccatgcc catgggcatg ccgggatggc ccgatttggc
    10681 ggcttccact gcgtccatgg cgagcgcgcg gatagaattt gccatcgtgc gtaaggcaga
    10741 tgtcggtttt tcgcccatat gaactgggct taaggcacgc ttcatccctg tgcaagggga
    10801 cgaaaggtcc gtccatcaat tctgccgggg attacacaag gattcaaaat cgcgccggtt
    10861 gcccacctgt taacgagtcc ctaaggtatc taccacttgc gcgataatcg gatgatctca
    10921 tgagcaagct tgacgatgcc ctggcacgct tccaaagggc agtagacctg ctcgaggcgc
    10981 gcgccgacga atgtgtcgcc aatgcctatg aaaccgctgc cgtagtcgcg gagtcgggtc
    11041 agtggaaggg cgaacgcgag agactggccg cgcgcattgc cagcctcgaa gaagaatcgc
    11101 gcacacttgc gggcctgacc gaggaggtcg aaggccggct cgacggcgcg attaccgaaa
    11161 tccgtgcggt cttggggcgc aactgacagg agtgggccca tggcggcggt caatgtgatg
    11221 gtgaatggtc aagcctaccc tgtcggctgc ggcgagggcg aggaagccca tgttcgcgaa
    11281 cttggccaat tcatcgacaa gcgcgtgcgg gatattgccg gttccatcgg tcaggtgggc
    11341 gatacgcggc tgcttctcat ggcagggctc gcgattgccg atgaactggc cgacgcgcgc
    11401 gccaagcttc gcgaacgcga atccgatttg ctggcattga gatccgagct gtcgggcgcc
    11461 gcggaaagcc gccaaaagag cgaggatatc gccgcggctg cgctttccgg agccgcttcg
    11521 cggcttgaag ccattgccgc gcgacttgcg catgcctaag ttatcgatgg gcgggtactg
    11581 ccccgcatgt caggcacgcg atacccaggg gccttaatgg tactcaccgg gagctgtccc
    11641 tggccgggat cgtggtcccg gtcatatggc gcccacctgc acttgcaggc ccgtgaggat
    11701 ccaagtccaa cggcgatggc ggtcccgccc actttcctct agtagctttc tcttatgaac
    11761 atcgccctct tatgaacatc gccgagtcgc aggcccttgc cgccgccaag gaagacgtca
    11821 ggaaagaagc gctggcgcgc cgcgccgtgc ttcagccggg tgtggcggag ctttcgcgcc
    11881 gtttggccca ggttttcgtg aactcggtcg ctatcccgcc gggcgcggtg gtttcggctt
    11941 atttcgccat cggcgatgaa gccgaccctc agcctttgct ggatttgctg cgcgcccgag
    12001 gtcacaaaat cgcgttgccg cgcgtggtgg gacggggcaa gccgctcgat tttcatctcg
    12061 tcgagacggg agcggagctg gttcggggcg gtttcggttt gtcggagccg catcgcgatt
    12121 ggcccaaggt cgatcccgat gtcctggccg tgccgcttct ggcgttcgac caggctggct
    12181 atcgcgtcgg ctatggcgcg gggttctatg accgcacgct gcaacggctt cggtcttcca
    12241 agaccatcat cgctgcgggc tatgcgttca gcgctcagga gtttcccaat ttgccgcatg
    12301 atgagaacga tcagaggctc gattggatgg cgaccgaaaa aggcgcgcgg agcttcgcgt
    12361 agacgggaca cgaaggacac gaaggatgca caaagatcac aggttccgcc gcgcgtgttc
    12421 tttgtgttcc tctttttggc attagaaacg gaaggcggat aatcgcgtat gcggattctt
    12481 ttccttggcg atatcattgg gcgccccggg cgcgatgtgc tcgccgccga gcttccgcgt
    12541 ttgcgcgaac gcctgacaat cgatttcgtc atcgccaatg gcgagaacgc ggccggcggg
    12601 ttcggaatca cgcgcgtgat cgccaatgag ctgttcgcca ccggcgtcga tgtgctgacc
    12661 agcggcaatc attggatgga tcagcgcgag attctcacct tcatcgatgc cgaagaacac
    12721 at
//
LOCUS       DASYEG010000256         2536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2847409, whole genome shotgun sequence.
ACCESSION   DASYEG010000256 DASYEG010000000
VERSION     DASYEG010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2847409"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VGM72_11075"
     CDS             <1..433
                     /locus_tag="VGM72_11075"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEY4265850.1"
                     /translation="DFDGANELPDPSLDYKIAFDINSMPQSATEVNPAIKMVGALINA
                     YEGHGVSPSHLHLVAVFHGPTIQVVLDDATYKERTGVEHNPNVELINQLKAHGVQMVV
                     CGQSTMAQHYNFKSIAHAKINFSASVTFINLMTRGYIKINE"
     gene            673..1938
                     /locus_tag="VGM72_11080"
     CDS             673..1938
                     /locus_tag="VGM72_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265851.1"
                     /translation="MPHPALETEHDDLLAIAGTMETAVPGLRVRLGGWREHLAHTLGK
                     ASSPFAAETLMEQATVALRHVWLEAAASSTSRSYRSPAARERTVTPVGPFHNFGYERD
                     LHPDALEARCANFFPPPPPGWRAEHVLFSSGQAAMNALLTLLGASAGRRLTLRHDGCY
                     FETTELLALARARFEVSARKPADIVIAEPIWYDGAQFGTTSLPGVAARANVERASHII
                     IDSTLSGLDDGVNDLLSTLDGGTQVFRTLSGLKLFQAGLELADVGIVSVYGAARADEL
                     RHIRTLQGTGLRFADVAALELPLFLDAAATRAYEHAIFTHNAALADAARGNPAFTTVY
                     PTGPRPAPFVVFNLPSAADYDGLDDKIAAEAARRELVLIRGGSFGFRGHRFETVRPTG
                     KPPFLRVAMGKRAGPSLDDILDLFRSFAP"
     gene            complement(1948..2175)
                     /locus_tag="VGM72_11085"
     CDS             complement(1948..2175)
                     /locus_tag="VGM72_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265852.1"
                     /translation="MKKPFSNENAVPGPKGDATDAQASICSRELAIYIAEMLKSLRTC
                     AKSPDLRTLEALLGAAEAEARNVVSGKSGKG"
     gene            complement(2311..>2536)
                     /locus_tag="VGM72_11090"
     CDS             complement(2311..>2536)
                     /locus_tag="VGM72_11090"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY4265853.1"
                     /translation="RSSGGDVLLTHEFLAVMLGTRRPGITAALSEMEKGSVIGTRRGS
                     ITVLDRAALEETANGSYGGAEAEYHRLFER"
BASE COUNT          455 a          848 c          762 g          471 t
ORIGIN      
        1 ggatttcgac ggcgcgaacg aactgcccga tcccagcctg gattataaga tcgcgttcga
       61 catcaattcg atgccgcaat cggcgaccga agtgaatccg gcgatcaaga tggtcggcgc
      121 gctgatcaat gcctatgaag gtcatggcgt gtcgcccagc catctgcatc tggtcgcggt
      181 gttccacggc ccgaccatcc aggtcgtgct ggacgacgcc acctacaagg aacgcaccgg
      241 cgtcgagcac aaccccaatg tcgagctgat caaccagttg aaggcacatg gcgtgcagat
      301 ggtggtgtgc ggtcaatcga ccatggcgca gcattacaat ttcaagagca tcgcccacgc
      361 caagatcaac ttctcggcct ccgtcacctt catcaacctg atgacgcgcg gctatatcaa
      421 gatcaatgag tgaggagcgg ttcgcgtagc gaacaaaatg gtccagtgga ccattttgag
      481 cgacgaacgc ccggagcgta agcgaagggc cggatagccg ctgcacatac cagctttctt
      541 cccccgcatg gcgaagccat tggcggggga agatgtcttt cgagggttca tcccgagaaa
      601 gacagatggg ggctgcaaag aaaacccttc cgggcttttt ctttgaacta agacgcgcaa
      661 caatccccct ccatgccgca ccccgccctc gaaaccgaac acgacgacct cctggccatc
      721 gcggggacga tggaaaccgc cgtgccgggt ctgcgcgtgc gcctgggcgg ctggcgcgag
      781 catctcgccc acacgctcgg caaagcgtcc tcgcccttcg ccgccgaaac gctgatggag
      841 caagcgacgg tcgcgctgcg tcatgtctgg ctggaagccg ccgccagttc gaccagccgt
      901 tcctatcgct cgcccgcggc gcgcgagcgc accgtcacgc cggtgggccc gtttcacaat
      961 ttcgggtacg agcgcgatct gcaccctgac gcgctggaag cccgctgcgc caacttcttc
     1021 ccgcccccgc caccgggctg gcgcgcggag catgtgctgt tctccagcgg ccaggcggca
     1081 atgaacgcgc tgctcacttt gctgggtgcg agcgcggggc ggcggctgac cctgcgccat
     1141 gacggctgct atttcgagac caccgaactg ctggcgctcg ctcgcgcccg cttcgaagtc
     1201 tccgcgcgaa agcccgccga catcgtgatc gcggaaccca tttggtacga cggcgcgcag
     1261 ttcggcacca catcactgcc cggcgttgcg gcccgcgcca acgtcgaacg cgcatcgcac
     1321 atcatcatcg acagcacctt gtcggggctg gacgatggcg tgaacgatct tctctccacg
     1381 ctcgacggcg gcacacaggt ctttcgcact ttatcgggcc tgaagctgtt tcaagcgggg
     1441 ctagagctgg ccgatgtcgg catcgtgtcc gtgtatggcg ccgcgcgggc ggacgaactg
     1501 cgccatatcc gcaccttgca gggcacgggc ctgcgctttg ccgatgtcgc ggcgctggaa
     1561 ttgccgctgt tcctggatgc ggccgcgacg cgcgcctatg aacacgcgat cttcacccac
     1621 aatgcggcgc tggccgatgc ggcgcgcggc aatcccgctt tcaccaccgt ctatcccacc
     1681 ggcccgcgcc ccgcgccgtt cgtggttttc aatctcccat cggcggcgga ctatgacgga
     1741 ctggacgaca agatcgcagc cgaagccgcg cgccgcgagc tagtgctgat acgcggcggc
     1801 agcttcggct ttcgcggcca ccgcttcgaa accgtgcgcc cgaccggcaa accgcccttc
     1861 ctgcgcgtcg ccatgggcaa gcgcgccgga ccatcgctgg acgacatctt ggatctgttc
     1921 cgcagctttg cgccgtgaaa gctgcggtca gcctttgccg gatttgccgc tcacgacatt
     1981 tcgcgcttcg gcctcggccg cgcccagcaa tgcttccagg gtgcgcagat cgggcgactt
     2041 ggcgcaggtg cgcagcgatt tcagcatctc ggcgatatag atcgccaact ctctagagca
     2101 gatcgatgcc tgcgcgtctg tcgcgtcgcc tttcggaccc ggcacggcat tttcattgct
     2161 gaatggtttt ttcatgccag ccctccatgg ctccggtttc gccgagatcg aagaagcgca
     2221 atcgcgcatg cccagttttc cgggaatgcc gatgaaaaac acatgtctgg aattggacca
     2281 tccggcgcat cgcggatggt gctgttcata tcaacgctcg aacaggcggt gatattccgc
     2341 ctcggcgccg ccatagctgc cattggcggt ttcttccagc gcggcgcggt ccagcacggt
     2401 gatggaaccg cgcctggtgc cgatgacgct gcccttttcc atctcgctca acgcggcggt
     2461 gatgccgggc cgccgggttc ccagcatcac ggccagaaat tcatgggtca gcagcacatc
     2521 gccgccgctg gagcgg
//
LOCUS       DASYEG010000257         4085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1253253, whole genome shotgun sequence.
ACCESSION   DASYEG010000257 DASYEG010000000
VERSION     DASYEG010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253253"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VGM72_11095"
     CDS             complement(<1..240)
                     /locus_tag="VGM72_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265854.1"
                     /translation="MRRALFHSLFAALLIAALPMPVWAAAPAHTGAAPIAPVFSSAEK
                     ADLKRVSAYLNTIQSVQGRFTQVDADGKTERGAFYL"
     gene            357..1388
                     /locus_tag="VGM72_11100"
     CDS             357..1388
                     /locus_tag="VGM72_11100"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY4265855.1"
                     /translation="MAPVPTSDRHPNPGYRSIAVVGAGAWGTALALVAAGNTPKVLLW
                     ARESEIVESLTRGRENAMFLPGVSLPECIEATGNIERARDAEAILLVAPAQHVRAVLS
                     ALRGMLKPATPVVLCAKGIETGSGLLLSEVLTEILPEAEPAILSGPSFARDAARKLPT
                     AVTIAARESVATRLQASLSQAWFRPYVSDDVVGVALGGAAKNVYAIACGMVEGAGLGE
                     SARAATISRGFAELMRLGAALGARSETLMGLSGLGDLVLTATSMSSRNFSLGVALGKG
                     ASLGDISGTGHPLAEGAATAPALVGRARRHGVELPIAEGVAAVLAGKLGVQDAAERLL
                     SRPLKAEAR"
     gene            1391..1804
                     /locus_tag="VGM72_11105"
     CDS             1391..1804
                     /locus_tag="VGM72_11105"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEY4265856.1"
                     /translation="MAYWLFKTEPEAWSWDQQKKKGAKGEPWSGVRNFQAAKNMKAMK
                     TGDLGFFYHTGEERQIVGILEVIGEYRKDPTDDTGRFGLVEVQAVKDVPKPVSLAVIK
                     ADPAFADMVLVKYSRLSVQPVTPDEWKRICEMGGL"
     gene            1801..2187
                     /locus_tag="VGM72_11110"
     CDS             1801..2187
                     /locus_tag="VGM72_11110"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY4265857.1"
                     /translation="MTPVFLCRIDSLEDPGTQNVVLGEGEGELDIVIVQTQGTRRAYI
                     NCCPHQFIPLEVFPNHFLTDDKSALVCSGHGARFALATGACISGPCLGQGLDPLAIVE
                     KDGAIYLAEPLEPIEIARNKRLSRRW"
     gene            complement(2188..2886)
                     /locus_tag="VGM72_11115"
     CDS             complement(2188..2886)
                     /locus_tag="VGM72_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEY4265858.1"
                     /translation="MKSAKSPAEFQLLLRRIAAETLGSAFLLAAVVGSGIMATRLTQD
                     VGLALLCNTLATAAVLFVLIVIFTPVSGAHFNPAVTFVAALNRTIPARIALFYVLAQV
                     IGTIAGVWLAHVMFGEAVFAWGTKARTGLGQNLSEAVATFGLVLTIAGTAARGRETAA
                     AAVALYITSAYWFTASTSFANPAVTLARALTPSFAGIRPFDAPAFIVAQLAGALLAAA
                     MARFMFPAPAERRS"
     gene            complement(2861..3364)
                     /locus_tag="VGM72_11120"
     CDS             complement(2861..3364)
                     /locus_tag="VGM72_11120"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEY4265859.1"
                     /translation="MSQEHPYNILFLCTGNSARSVLAEAYLNSTAATRFHAYSAGSHP
                     KGAIHALTLDTLRIAHLPVWGLRSKSWDEFAQADAPKMDFVITVCDSAAGEVCPIWPG
                     QPITAHWSFPDPAAFEGPEVEQRAFFLDVFRQIRTRLDLFRNLPIARLGRLSLKREID
                     EIGQKPG"
     gene            complement(3361..3687)
                     /locus_tag="VGM72_11125"
     CDS             complement(3361..3687)
                     /locus_tag="VGM72_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY4265860.1"
                     /translation="MKYTHAIAALSALSQETRLSVFRHLVAAGPQGRPAGLIAARLRI
                     PAPTLSFHLKELERAGLITRHRESRSIIYAANYAAMRALLSYLMEDCCGGRPEICNIE
                     VKCCAA"
     gene            complement(3728..>4085)
                     /locus_tag="VGM72_11130"
     CDS             complement(3728..>4085)
                     /locus_tag="VGM72_11130"
                     /inference="COORDINATES: protein motif:HMM:NF013011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliP"
                     /protein_id="HEY4265861.1"
                     /translation="PIKKFMGAHTRNEDLSLFVNMAGVPAPASAEGAPLQAIVPAFML
                     SELKRAFEIGFLIFVPFLVIDMAVASILMSMGMMMLPPVVISLPFKLIFFVLVDGWRL
                     VAGSLVASYGVGIAPQ"
BASE COUNT          833 a         1204 c         1324 g          724 t
ORIGIN      
        1 cagatagaag gcgccccgtt cggtcttgcc atcggcatcc acctgcgtga agcgtccctg
       61 cacgctttgg atcgtgttga gataggccga aacgcgcttc agatcggcct tctcggctga
      121 ggagaaaacg ggcgcgatcg gcgcggcgcc cgtatgggcg ggggccgccg cccatacggg
      181 cattggaagt gccgcgatga gcagggctgc gaagagactg tgaaaaaggg ctcggcgcat
      241 gatcgccttt ctaggaaatt tttatggcgg aaagaaggga ggcggtgatg cggctccgtt
      301 tattcggcta taagcctgat ctcccgatca taatccgcga aaaccagcac cgttccatgg
      361 cgcccgtccc cacctccgac cgacatccga accctggcta caggagcatc gctgtggtgg
      421 gcgccggcgc ctggggaacg gcgctcgccc ttgtcgcggc cggcaatacc ccgaaggtcc
      481 tattgtgggc gcgcgaaagc gagatcgtgg aatcgctgac ccgcggacgc gagaatgcga
      541 tgtttcttcc gggcgtctcc ctgccggaat gcatcgaagc cacggggaac attgagcgcg
      601 cgcgcgatgc ggaagcgatc ctgttggtgg cgccggcgca gcatgtgcgc gcggtgctga
      661 gcgcgttgcg cggcatgttg aaaccggcca ccccggtcgt gctctgcgcc aagggcatcg
      721 agacgggcag cggccttctc ttgagcgagg tgctcaccga gatcttgccc gaggccgaac
      781 cggccattct gtcggggccg tccttcgcgc gcgacgcggc gcgcaaactt ccgaccgcgg
      841 ttaccatcgc cgcgcgcgag agcgtcgcga cgcggctgca ggcgagtctc tcccaggcgt
      901 ggttccggcc ctatgtcagc gacgatgtgg tgggtgtcgc gttgggaggt gccgcgaaaa
      961 atgtctacgc cattgcttgc ggcatggtgg agggcgcggg gctcggcgaa agcgcgcggg
     1021 cggccacgat ctccagaggc tttgccgagt tgatgcggct gggtgccgcg ctcggcgccc
     1081 gctcggaaac cttgatgggg ctttcgggcc tcggcgatct ggtgctgacg gccacaagca
     1141 tgagctcgcg caatttttct ctcggcgtcg cactcgggaa aggcgccagc ctcggggata
     1201 tttccggtac cggccatccg cttgccgagg gcgccgccac tgcgcctgcc cttgtcggcc
     1261 gcgcgcgccg ccatggggtc gagcttccca tcgccgaagg cgtggccgcg gttcttgccg
     1321 gaaagctcgg cgtacaggac gcggcggaga ggttattgtc tcgtccgttg aaagcggagg
     1381 cgaggtaacc atggcctatt ggctgttcaa gaccgaaccc gaagcctggt cgtgggacca
     1441 gcagaagaaa aaaggcgcca agggcgagcc ttggagcggc gtgcgcaatt tccaggccgc
     1501 caagaacatg aaggcgatga agacgggcga tctcggtttc ttctaccata ccggcgaaga
     1561 gcgccagatc gtcggcatcc tcgaagtgat cggcgaatat cgcaaagacc cgaccgacga
     1621 tacgggccgg ttcgggctgg tcgaggtgca ggcggtgaag gatgtgccca aacccgtgag
     1681 tttggccgtc atcaaggccg atccggcctt tgccgatatg gtgctggtga aatattcgcg
     1741 gctttcggtg cagccggtca caccggacga atggaagcgc atctgcgaga tgggcgggtt
     1801 gtgacgccgg tctttctctg ccgcatcgat tctcttgaag atcccggaac gcagaatgtg
     1861 gtgctgggcg aaggagaagg cgaactcgat atcgtcatcg ttcaaacgca aggcacgcgc
     1921 cgcgcctata tcaattgctg cccgcaccag ttcatcccgt tggaggtctt tcccaatcat
     1981 ttcctgacgg acgacaagag tgcgctcgtc tgttcggggc atggcgcgcg ctttgcgctc
     2041 gccaccggcg cgtgcatcag cgggccatgt ctcggccaag ggctggaccc tctcgccatc
     2101 gtggaaaaag acggtgcgat ctatctggcc gagccgcttg agccgatcga gatcgcccgc
     2161 aacaagcgcc tcagccgccg ctggtgatta gctgcgacgt tccgcaggag cggggaacat
     2221 gaagcgggcc atggccgcgg ccaacaaagc tcccgccaat tgggcgacga taaaggctgg
     2281 cgcatcgaag ggacgaattc cggcaaagct tggcgtcaga gcgcgcgcca acgtaaccgc
     2341 tggattggca aaggacgtcg aggcagtaaa ccaataggcg ctggtgatat agagcgcgac
     2401 cgccgccgcc gctgtttcgc gacctcgcgc cgctgtgccc gcaatggtca gaacgaggcc
     2461 aaacgtcgcg acggcttccg aaagattctg cccgaggccg gtccgcgcct tggtgcccca
     2521 ggcaaagacc gcctcgccga acatgacatg cgccaaccag acgccggcta tggttccgat
     2581 gacttgcgcc aggacataga aaagtgcgat ccgcgccgga atcgtacgat taagggcggc
     2641 tacaaacgtc acggcgggat tgaaatgcgc gcctgaaacc ggcgtaaaga tcacgatgag
     2701 cacgaacagc accgccgccg tcgccagcgt gttgcaaaga agcgcgagcc caacatcttg
     2761 cgtcaggcgt gttgccataa tgcctgagcc caccaccgcg gcgagtagaa aagcgctccc
     2821 aagcgtttcg gctgcgatgc ggcgaagcag gagttggaac tcagccgggc ttttggccga
     2881 tttcatcgat ttctcgtttc agcgaaagcc gtcccaaacg cgcaatcggc agattgcgga
     2941 aaagatcgag gcgcgtgcgg atttgacgaa acacgtcgag gaagaaggcg cgctgctcca
     3001 cctcgggtcc ctcaaaggcg gccggatcgg gaaagctcca atgcgccgta atcggctggc
     3061 ccggccagat cgggcaaacc tcgcccgccg ccgaatcgca aaccgtgatg acgaaatcca
     3121 tcttgggcgc atccgcttgc gcgaattcat cccagctttt ggagcgcaaa ccccataccg
     3181 gcaaatgggc gatgcggagt gtatcgagcg taagcgcatg aatggcgcct ttgggatggc
     3241 tgcccgcgct ataggcatga aagcgggttg ccgctgtcga attcagatag gcctcggcga
     3301 ggaccgaacg cgccgaattt ccggtgcaga ggaacaggat attgtaggga tgctcctggc
     3361 tcatgcggcg cagcacttca cttcgatgtt gcagatttct gggcggccac cgcagcaatc
     3421 ttccatcaga taggaaagca gcgcccgcat cgccgcataa ttggccgcat agatgatgct
     3481 gcggctttcg cgatgccggg tgatcagccc ggcgcgttcc aattccttga gatgaaagga
     3541 cagcgtcggt gccggaatcc ttaggcgcgc cgctatcagc cccgcaggtc tgccttgggg
     3601 gccggccgca accagatgac ggaataccga aagacgggtt tcctgtgaaa gggcggagag
     3661 ggcggcaatg gcatgagtat atttcatagt ttcataacta tgaaattatc aactcttggt
     3721 caagttccta ttgcggagcg atgccgacgc cgtagctggc gaccagcgag ccggcgacga
     3781 gccgccagcc atccaccaag acgaagaaga tcagcttgaa aggcagcgag atcacgaccg
     3841 gcggcagcat catcataccc atggacatga gaatcgatgc caccgccatg tcgatgacga
     3901 gaaacggtac gaagatcaga aatccgattt cgaacgcacg cttcaattcc gaaagcataa
     3961 aagccggaac gatggcctgc agcggtgcgc cctcggcgga ggctggggca gggacgcccg
     4021 ccatattgac gaatagcgaa agatcctcgt tgcgcgtatg cgcgcccatg aatttcttga
     4081 tcggt
//
LOCUS       DASYEG010000258         5528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_819510, whole genome shotgun sequence.
ACCESSION   DASYEG010000258 DASYEG010000000
VERSION     DASYEG010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5528
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819510"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VGM72_11135"
     CDS             complement(<1..704)
                     /locus_tag="VGM72_11135"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265862.1"
                     /translation="MRIGVTYDLRADYLARGMSEEESAEFDAEITIASICEALSGLGH
                     EPVRIGHIRALTQRLAADERWDAVFNICEGLSGYAREAQVPALLEAYAIPVVFSDPLT
                     LALTLDKAWTKRIVRSAGVPTPDFAVIETPADIDGVNLPFPLFLKPVAEGSGKGVSER
                     SRVDTQSGLRATASDLLMKFRQPVLAETYLPGREFTVGITGTGQDASALGVMEIKPTA
                     QGTQSGYSYENKEHWE"
     gene            complement(732..2051)
                     /locus_tag="VGM72_11140"
     CDS             complement(732..2051)
                     /locus_tag="VGM72_11140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HEY4265863.1"
                     /translation="MNLQLLRGRDLRPPSRRERLPAAVSTTVLQTHTAQTHLEAPARK
                     KILSFPVNRDTRAFYRHFYPDTAAAEWNDWRWQMRSRIRTLAELERVFALSADERAAV
                     SGHSGSLPVGITPYYASLMDLKDAMEPLRRTHIMVKDEYVRLPGEEDDPLGEDHDTVV
                     PGLVHRYPDRVLFLTTGTCSTYCRYCTRSRLVGNPGGEYQFNVRNWELAFDYLEAHTE
                     VRDVLLSGGDPLTIGDDKLDYLLGRLRAIKHIEFVRIGTKIPTVLPQRITKDFVKMLK
                     KHHPLWMSLHFTHPKELTPEVSESTSRLVDAGIPLGSQTVLLKGINDDIAVMKPLMHG
                     LLMRRVKPYYLYQCDPIKGSGHFRTSVEAGLEIIKGLRGHTTGYATPMFVIDAPGGGG
                     KILMAPDSVVGREGDDLLIRNFEGNVYRYPDPGGALGGDKAMPLAAE"
     gene            complement(2553..4220)
                     /locus_tag="VGM72_11145"
     CDS             complement(2553..4220)
                     /locus_tag="VGM72_11145"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY4265864.1"
                     /translation="MTESLRACVRRQPLAVLLVICVLAWVPGFFILPPLDRDESRFAQ
                     ATKQMLETRDFVDINLGGVARYEKPVGIYWLQAASTALLGGGVRDAIWTYRVPSLLGA
                     LGAVAAAYWLASLFASVETAFFSGLILGLSVLLMSEAKIAKTDAVLLGTVTVAQAVIL
                     RSYLGARAGDGSAVPASRLFLGWGAFGLGVLVKGPLIAAVCLASVAAIVIWDREWRWL
                     ARLRPLLGLGIAAAIVLPWAIAIGIASHGLFYEKSLGQDFATKLMGDAESHGAPPGYY
                     TALVSFTFWPGTLLLLPGILYAFARRREPAVRYLLAWAATTWLMFELAPTKLPHYVLP
                     AYPALAVLCALWLTSQAREESRATRIAQYVSLTLFVLVGLALAGFLALAPQRLGGGSP
                     WWLYAAVALDAVAILAVVPRILARRFAAAAGMAGLGAILLYGIAGFLTVPRLTQLWLS
                     PRLAEAVARHAQPGDPPVVTAGYAEPSIVFLLGTRTALDGGPGAGHAAAAAGGLALIS
                     DDQRGAFLDAVARDGAMAAALEDVDGLNYSRGRKTRITVYRVMPHAR"
     gene            4328..5059
                     /locus_tag="VGM72_11150"
     CDS             4328..5059
                     /locus_tag="VGM72_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY4265865.1"
                     /translation="MSDIVEVSVVVPVKDEAGNVVPLAREIAAALGAERYEAIFVDDG
                     SRDATAAELLSLRGEMPVRVIAHGRNLGQSRAVRSGVRAARGSIVVTLDGDGQNDPAD
                     IPKLLSAFRAAAAADPDFGMIAGEREKREDSWKKRIASRYGNRIRRWMLSDNAKDTGC
                     GLKLFRREAFLALPYFDHMHRYLITLMLREGYQVRFMPVGHRPRGAGRSKYGVWDRAW
                     VGLADLIGVLWLKRRFRGPDKTVEL"
     gene            5064..5387
                     /locus_tag="VGM72_11155"
     CDS             5064..5387
                     /locus_tag="VGM72_11155"
                     /inference="COORDINATES: protein motif:HMM:NF019214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HEY4265866.1"
                     /translation="MGHWLNWMNADGGWLLVGFLGQGLFASRFIIQWLKSEAAGRSVV
                     PLAFWYCSIAGGTVTLVYTIHLRSAPLILGQATPLLIYVRNVYLILRERRALRPTLET
                     GTPGG"
     gene            5438..5527
                     /locus_tag="VGM72_11160"
     tRNA            5438..5527
                     /locus_tag="VGM72_11160"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5472..5474,aa:Ser,seq:gga)
BASE COUNT         1104 a         1751 c         1735 g          938 t
ORIGIN      
        1 ttttcccagt gctccttgtt ctcataggaa tagccgctct gcgtgccttg ggcggtcggc
       61 ttgatctcca tgacgcccag tgcgctcgca tcctggcccg tgccggtaat gccgacggtg
      121 aattcgcgcc ccggcagata ggtctcggca agcaccggct ggcggaactt cataagaaga
      181 tcggacgcgg tcgcgcgcaa gccgctctgc gtatcgacgc gcgaacgctc gctgacgcct
      241 ttgcccgatc cttcggcgac cggcttcagg aacagaggaa acggcaaatt cacgccgtcg
      301 atatcagccg gcgtttcaat caccgcgaaa tcgggcgtcg gcacccccgc cgaacgcacg
      361 atgcgcttgg tccaagcctt gtccagtgtc agcgccaagg tcagcggatc ggagaacacc
      421 accgggatcg cataggcttc gagcaatgcc ggcacctggg cttcgcgcgc atagcctgaa
      481 agcccctcgc agatattgaa gacggcatcc cagcgctcgt ccgcggccaa tctttgcgtc
      541 agcgcgcgga tatggccaat gcgcaccggc tcgtgcccga ggccggagag cgcctcgcag
      601 atcgatgcaa tggtgatctc cgcgtcgaat tcggcgctct cttcctcgct catcccgcgt
      661 gcgagatagt cggccctgag atcgtaggtg acgccgatgc gcatggcgca gtcagtccgc
      721 cgcggtccgc atcattccgc cgcgagcggc atcgccttgt cgccaccgag cgccccgccg
      781 ggatcgggat agcgatagac gttgccctcg aaattgcgga tgagcagatc atcgccctcg
      841 cgtcccacga cggaatcggg agccatcagg attttgccgc cgccgcccgg cgcatcgatc
      901 acgaacatcg gcgtcgcata gcccgtggta tgaccgcgaa ggcccttgat gatctcaagg
      961 cccgcctcga ccgaggtgcg gaaatggccc gagcctttga tcggatcgca ttggtacaga
     1021 taatagggtt tcacccggcg catcagaagc ccatgcatca gcggcttcat caccgcgata
     1081 tcgtcattga tgcccttcag cagtaccgtc tgcgacccca gcggaatacc cgcgtccacc
     1141 aggcgggagg tcgattcgga aacctccggc gtcagttcct tgggatgcgt gaaatgcagg
     1201 ctcatccaca aggggtgatg cttcttcagc atcttcacga aatccttcgt gatgcgctgg
     1261 ggaagcactg tggggatttt cgtcccgatg cgcacaaatt cgatgtgctt gatggcgcgc
     1321 agccggccca gcaaataatc gagcttgtcg tcgccgatgg tgagcgggtc gcccccggac
     1381 agcagcacgt cgcgcacttc ggtatgcgct tcgagataat cgaatgcgag ttcccaattg
     1441 cgcacgttga actggtattc gccgcccgga ttgcccacca gccgcgagcg cgtgcaatag
     1501 cggcaatagg tcgagcacgt cccggtggtg aggaacaaaa cccggtcggg atagcgatgc
     1561 acgagacccg gcacgaccgt atcgtggtct tcacccagcg gatcgtcttc ttcgccggga
     1621 agacggacat attcgtcctt gaccatgatg tgggtgcggc ggagaggctc catcgcatcc
     1681 ttcaagtcca tcaagcttgc gtaatagggc gtgatgccga cgggcagcga accggagtgg
     1741 ccggaaaccg cggcgcgctc gtccgccgag agcgcaaaga cgcgctcaag ctcggccagc
     1801 gtgcggatgc ggcttcgcat ctgccaacgc caatcgttcc actcggcggc ggcggtgtcg
     1861 ggatagaagt ggcggtagaa ggcccgcgtg tcgcgattga cggggaagct gaggattttc
     1921 ttccgggcgg gcgcctcaag atgggtttgg gcggtgtggg tttgtaagac ggttgtggat
     1981 acggcggcag gtaatcgttc acgccgcgat ggaggacgaa gatcgcgtcc tctcaagagc
     2041 tggagattca tcggtgtctc atgtcgctct tcatgccgaa cctcgacacc ccccgatggc
     2101 agtgcctatc gaacggtcca gcggtcctta gatttagagg gcttttcacg ttcgtcaaga
     2161 gaaaagccgg tgaatactgt gaaagcttac gcgaattcca atatagaggc atcgaattac
     2221 cccgaattat tgaacatgat gaaaagaaaa gccgaagttc gatgaagatt cacagagaag
     2281 acgcaccgag cagatgccag gaaagataaa aacttgtgca agcaaacgca cggccttccc
     2341 ttcatgcaag ctttgcgcga atcggttcgg ccgtattcat ggatgttttg acgcgcatac
     2401 ggactgtctt cgcaaagtca tcgccgcact ctcaacgcag cacccggacc gaaaaaacat
     2461 cgccgtcccc ggcacgagtc cggcacggca ggcgcgaacg atccacagcg aatcgatgaa
     2521 acggcgcggg gtgagcgaaa cccgcgcgcg actcaccttg catgaggcat gacgcgatag
     2581 acggtgatcc gagtctttcg gccgcgcgaa taattgagcc cgtcgacgtc ttcgagcgcg
     2641 gcggccatcg caccgtccct ggcaacggcg tcaaggaacg ctccgcgctg atcgtccgaa
     2701 atcaaagcga ggccgccggc cgccgcggcc gcgtggcccg cgccgggccc gccatcgaga
     2761 gccgtcctgg tgcccaggag aaacacaatg ctcggttcgg catacccggc cgtgaccacc
     2821 ggtggatcgc cgggttgcgc atggcgcgcg acggcttcgg ccaggcgcgg cgacagccac
     2881 agctgcgtca gccgcggcac cgtcagaaaa cccgcgatgc cataaaggag aatcgcaccg
     2941 agcccggcca tccccgcggc agcggcgaag cggcgcgcta ggattcgcgg aaccaccgcg
     3001 aggatcgcga cggcatccag cgccacggcg gcatagagcc accacggcga gccaccgccc
     3061 aagcgctgtg gcgcgagtgc gagaaatcct gcgagcgcaa gccccaccag gacaaacagc
     3121 gtgagcgaga cgtattgcgc gatgcgcgtt gcccgcgatt cctcacgcgc ctgcgaggtc
     3181 agccagagcg cgcagagaac cgccaatgcc ggatacgcgg gcagcacata atggggcagc
     3241 ttggtcggcg cgagttcgaa catcagccat gtggtcgctg cccaggcgag gagatagcgc
     3301 acagccggct cgcgcctgcg tgcgaaagcg tacaggatgc ccggaaggag caggagcgtt
     3361 cccggccaaa aggtgaagct caccagcgcg gtgtaatagc ccggcggcgc tccatgactc
     3421 tccgcgtccc ccatcagctt ggtcgcgaaa tcctggccca gcgatttctc gtagaagagt
     3481 ccgtggctcg cgatcccgat ggcgatcgcc caaggcagca cgatggccgc cgcgattccc
     3541 aatcccaaaa gcgggcgcaa ccgcgcgagc cagcgccatt cgcgatccca tatgacgatg
     3601 gccgcgaccg aggcgaggca gaccgccgcg atcagagggc ctttcaccag cacgcccaac
     3661 ccgaaggcgc cccagccgag aaacagacgc gaagccggca ccgccgagcc atcgccggcg
     3721 cgcgcgccaa gatagctccg caggatcacg gcctgagcca ccgtcacggt cccgagaagc
     3781 accgcatcgg ttttcgcgat cttagcctcg gacatcagga gcaccgaaag ccccagaatc
     3841 aggccggaga aaaatgccgt ctccacgctt gcgaacaggc tggccagcca ataggccgcc
     3901 gcgacggcgc caagcgcgcc cagcagggac ggcacgcgat aggtccagat cgcatcgcgc
     3961 accccacccc cgagaagtgc ggtcgaagcc gcctggagcc aatagatccc caccggcttc
     4021 tcataacgcg caacgccgcc gagattgatg tcgacgaaat cgcgcgtctc aagcatttgc
     4081 ttggtggcct gggcgaagcg gctctcatcg cggtcgagcg gcggcaggat gaagaacccc
     4141 ggcacccagg ccagcacgca gatcacgagc aaaaccgcga gcggttggcg gcgcacacat
     4201 gcgcgaagcg attcggtcat tgccctccac ccttcgtcca cggctcatga aatcggcgca
     4261 agcctgtttc ccggaaggtt ggacttgtat aaggcgcacg ccagaatccg aaaaggcatt
     4321 ctcacgcgtg tccgatattg tcgaggtttc cgttgtcgtt ccggtcaagg atgaggctgg
     4381 caatgtcgtg ccgctggcgc gcgaaatcgc cgcggccctc ggggctgagc gctatgaggc
     4441 gatcttcgtg gacgacggat cgcgcgacgc cacggcggcc gagcttctgt ccctgcgcgg
     4501 cgagatgccg gtccgggtga tcgcccatgg caggaacctc ggtcaaagcc gcgcggtccg
     4561 ctccggcgtg cgcgcggcca gaggcagcat tgtggtgacg ctcgatggcg atgggcagaa
     4621 cgatcccgcc gatatcccaa aactgctgag cgcatttcgt gcggccgcgg cggccgaccc
     4681 cgatttcggc atgatcgccg gcgaacgcga gaaacgcgag gacagttgga aaaagcgcat
     4741 cgcctcgcgc tacggcaatc gcatacggcg ctggatgctc tcggacaatg ccaaggatac
     4801 gggctgcgga ttgaagctgt tccgccgcga ggcctttctc gccctccctt atttcgacca
     4861 catgcaccgt tacctcatca cgctgatgtt gcgcgaaggc tatcaagtgc gcttcatgcc
     4921 ggtcggacac cgcccaaggg gcgcgggccg ctcgaaatac ggcgtgtggg accgggcctg
     4981 ggttggtctc gcagacctta tcggtgtgtt gtggctgaaa cgccgctttc gcggcccgga
     5041 caagaccgtg gagctctagc gcaatgggtc actggttgaa ttggatgaac gccgatggcg
     5101 gctggcttct cgtcggcttc ctgggacagg ggctcttcgc gtcccgcttc attatccaat
     5161 ggctgaaaag cgaagccgcg gggcgcagcg tcgtgccgct ggccttctgg tattgcagca
     5221 tcgccggcgg gacggtgaca ttggtctaca cgattcatct gcgaagcgcg cctttgattc
     5281 taggccaggc cacgccgctg ctgatctatg tccgcaatgt ctatctgatt ttgcgcgagc
     5341 ggcgggcgct ccgccccacg ctcgaaaccg ggacgccggg cgggtaacac ggcgcttgcc
     5401 ggagcgaaac gagccgctat agtccggcgc gctttgcgga caggtggccg agcggctgaa
     5461 ggcgcacgct tggaaagcgt gtttacggga aaccgtaacg agggttcgaa tccctctctg
     5521 tccgccac
//
LOCUS       DASYEG010000259        14245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_384494, whole genome shotgun sequence.
ACCESSION   DASYEG010000259 DASYEG010000000
VERSION     DASYEG010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 14245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14245
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384494"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VGM72_11165"
     CDS             <1..993
                     /locus_tag="VGM72_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265867.1"
                     /translation="SANLPIGEATSFSFDMRRNPEDGIVPFQTLAGPQDHLTHVTVMR
                     ISGVAEGIGWTAGNGLSLHDGMMGDGGSPFAGASLTNAFSPLVRAAPGAMASFRLPLS
                     PSTGLSFGAGFSENQGLTDHLRTPFRNSAQSAEMRLDHVAGQTRFSLEAGDVLETGGF
                     MGSLAAGGLTMAAQASTVWTTASAEVTLDAHWSLKGALTLAATAASHTQASLITAIGP
                     VYATSFALGLAGENLFRGGDVLSFTLGQPLRAERGTLTLVSGVSRDWTDGGMIMGETQ
                     ASLEPSGREFDLETGYRFDLRGWGFGANVAYAIDPNHVQNASAVVALFTLTRGF"
     gene            complement(1006..2499)
                     /locus_tag="VGM72_11170"
     CDS             complement(1006..2499)
                     /locus_tag="VGM72_11170"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY4265868.1"
                     /translation="MHGTNATMAYMTGHMLIGGELIEAADGGWLESINPADETQLGRV
                     PMGTKADMDAAVAAAEAAQPAWAALTMGQRAEYIHKLGDAILKRGDEIARIESLDTGN
                     TLGPMKRDVRTAVERMRFAAGLAYEIKGETIPATSGNIHMTIRQPYGVIGRIIPFNHP
                     IGFAASRIAPAIISGNAIVVKPSEQSPLSASILGEICKEVLPPGVVNIVTGARETGEA
                     LVRHPRVKRIAFIGQPASGMAIQKAAAETAVKLVTLELGGKNPLIAFEDADLDKVAAC
                     SVGGMNFGWQGQSCGSMSRILLHESIHDRVVEKILERVRKIRIGHPLDPATNMGPINN
                     KAQYAKVLAYIDLGHKEGGKLLHGGGIPKGEEFQRGYWVEPTVFGGMDMSMRLAREEV
                     FGPVMSIFKFGRETEALQMANAIELGLTAAVWTNDINRALRVAQQVQAGYVWVNGVGQ
                     HYRGVPYGGFKNSGIGREEGISELLSYTEEKVINIVLGGSDYNPAAG"
     gene            complement(2573..3523)
                     /locus_tag="VGM72_11175"
     CDS             complement(2573..3523)
                     /locus_tag="VGM72_11175"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY4265869.1"
                     /translation="MEKRKLGRSALEIAPVVFGGNVLGWTVDQASAFRILDAFVDAGF
                     NALDTADTYSRWIPGNKGGESETIIGNWLKQGGGRRERVLILTKLGAEMGPDKKGLSR
                     RYIKAEIEDSLRRLQTDTIDLYQSHRDDISTPMLETLETYAELIREGKVRAIGASNFS
                     AARMREALDVSAKHGLPRYECLQPPFNLYDRDDYEGALEQLCLKENIGVIPYYGLAAG
                     FLTGKYRSAVDFGKSPRGGGMSNYLNAKGKKILTALDAVAAAHHVKPAAIALAWLIAR
                     PSITAPIASATSIEQLGDFAAAAALKLFPDEIATLDVAGA"
     gene            3603..4664
                     /locus_tag="VGM72_11180"
     CDS             3603..4664
                     /locus_tag="VGM72_11180"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HEY4265870.1"
                     /translation="MIFAIKHMIATSRFSLPRIVGIFAVLIGANIAVWIWAIAAFQHY
                     PVLLGTALLAYSFGLRHAVDADHIAAIDNVTRKLMQSGKPSASVGLFFSLGHSTVVIL
                     ASLLIALGAGALNARWSAFRDVGGLIGTSVSVLFLFAIAIANLFILAGVFRTFQYVKR
                     GGRLAEDDGGMLFAGGGLLARIFRPLFGFIRASWQMYPLGVLFGLGFDTATEIGLLGI
                     SAAQAARGLSILPLLVFPALFTAGMALVDTADSVLMTRAYGWAFVKPIRKLYYNLTIT
                     AVSVLVAVLVGSVEALGLIADRLALKGAFWDGIEALNSQLGVLGYLIIALFAVSWIVS
                     VLIYRLKGYDGLDIAGETP"
     gene            complement(4695..5054)
                     /locus_tag="VGM72_11185"
     CDS             complement(4695..5054)
                     /locus_tag="VGM72_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY4265871.1"
                     /translation="MAMPIRSLTLGVVLAVLSFGAVQAQDTPRGDPKAGLVNFKKLGC
                     YTCHGIIGQGTLRDGPHLNAAALGFPAVLAQLRNPRYEMPIYTASQISDAGVADIFAY
                     LSSIPKGPDPKSIKQLQ"
     gene            complement(5054..6982)
                     /locus_tag="VGM72_11190"
     CDS             complement(5054..6982)
                     /locus_tag="VGM72_11190"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY4265872.1"
                     /translation="MSKTIKRRQFLAGMAAAPAAGLAATAGKAVAQTTPAPVSSVPPV
                     PMTDRPSSDTPPQTIGKTGSDFMVDVLKSLDIDYIASCPGSTFRGLHESIINYGDNKK
                     PEFITTLHEDTAAAMCHGYYKVAKKPMACMVHGTVGLQHATMAMYNAFADRVPMLVLT
                     GNIGKQETRQAYIEWDHAAQDQGAMVRDFTKWDDQPASLQGFAESTVRAYDMMTTAPM
                     APVLIVADGDLQEDPIPAEVEKKLHIPKLKMRFQPAGDIAAVREVAKMLIAADSPVIF
                     ANRYGRTENAPALLIQLAELIGAPVVDSHNRMNMPNRHPLNHSLRREAALQSADVILA
                     LEPIDLFGLNNYVPDLVTRPNDPRRRQANLKIIHIGTEGMMAKSNFQDFQRFAAADLS
                     IAGDAEATMPALIEALKTQMSVADKNRCDGRCAKVKAQTAKLLDQSRQEAAYQWETQP
                     ISSARLTMELWDQIKNEDWMMPTETQFLSDWPYRLWDIDKPYRTMGGSGAQGIGYNSP
                     AALGAALANKDKGRLTVAMVGDGDLNMAPGVLWTAAHHKIPILYLVHNNRAYHQEVMM
                     ILRMASRRQRVNNKNVYVGTLIDDPAPDYAKIAQGYGLYAQGPVSDPKDLTAAIRRAI
                     DVVKKGEPALIDIVSDGR"
     gene            complement(7115..8434)
                     /locus_tag="VGM72_11195"
     CDS             complement(7115..8434)
                     /locus_tag="VGM72_11195"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4265873.1"
                     /translation="MQTDSLSQAYEHFKSGHFDAAEERACDLLDANPAHAEANHLLGA
                     IRFQQGRIEESLAFLKRAAASPRATAELHNNLGAVLSKLGRKDEAVAAFERALAMNPA
                     YADALNNLGVIYLETQKPEQAVSAFRKAVVLEPGLLHATANLRATYQGVVPSWHFAMM
                     DDHKRNGAYEAAIRRAAPGKRVLDIGTGAGLLALMAARAGASKVTTCEAVSLIAERAR
                     EIIVRNGLSDRIGVVPLKSTAMSVPGIMPERAEVLVTETFASGLITEGVLPAIEHAHE
                     YLLVPDAAIIPAAASIMGYLAGGETLQGMLFAGRIAGFDLSGFNDFAPAMLAVALGNI
                     PHRVLSGDVEVMRFDFREKRFPMGGVRLALKATQHGICVGVAQWIRLELDAHATYENR
                     PSPNAPFNGHWTHILHRFPRPVTVAPGDVVPVLFRHDRSQIGIDLID"
     gene            complement(8645..11902)
                     /locus_tag="VGM72_11200"
     CDS             complement(8645..11902)
                     /locus_tag="VGM72_11200"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4265874.1"
                     /translation="MTPSRIAQAQVAPQQPAQIQLAQVQVAQAQVAQAQTAQAQTAQA
                     APGEVPEQVLITGSLIRGTAAVGVPVTNLSVQDIRMTGSLTTSDLFRTIPQFNVIPGP
                     VGTQAANVERGTRVNLRQLDTGSAPRSLMMIDGVRYPAQGMGLCQIDPSIIPTVAVDR
                     IDLLLDGASATYGSDAIGGVINIILKRGYDGAQTEIGFTTGAGGNTKYLASQLWGRTW
                     DGGDITIGYQWYDVAPTHGNFNSKYTFDFTPWGLDNRTPLASAMPGIISTGAPAAPDQ
                     TNYPATNGHNCTNCFSIPQGTGRDFNTINGGLGPLGGSTAATINWATFANPANGGPVN
                     GTANEFNPYSITDYSAASQYVGSSVTFDQRLTKDISFYGEGFYGMRRSRFINNTVGNQ
                     LTLAVPTFNPYYPTNAPNGLRVSYNFSIESPSITNSYASAQRYLGGLNIDLPGDWAAQ
                     VYYSQTRDAEYNHVVGAVNRAAVSAALGWTMPATPATGTNPTIGTFTKPPTTPYLNLF
                     CDTTTYQCNSPSTLAFIGAYNETAEAFWVDEKGFKADGPLFDLPGGPVKMAVGANYTS
                     YRFLIQSNTETSTSNTAMTLLRDPESRRVWAVFGQLNVPVIGDNNSLPGVRKLELEAS
                     WRHDQYSDVGGTSNPKLAFNWEVSEDLGLTFRGSWGTSFRAPSFGEFSPISNVAWNGW
                     GLQNAPGAAQFQNNATIQVACDASGKPPAGSGAEKLFNAGFACNSQPAGLSLNGGGKA
                     AVDAHFRTYFNQDNQVLRPELSTNWGFGFDFAPTSFLRGLDVQATWYTVKINGILINF
                     GNPTTNRFNDSSLAFVYLVPSDVGCPVAQNAHPEQCAAFQDMVQRALAHPTDPVPSAA
                     QSLIYWINDGGTLNKGWQKTEGIDWSISYDWDLGDLGAWNTGMTGTYYLKQESVRVPG
                     AAGPAGQVTDVLYDTDLASVGGVPQLGVESLPRFKYRARLGWSNGPWSVTGFMDYQGH
                     FYHTQTAPPNVNFECLTAGGSVGGGTFPCALNNYTNIEPSYYTFDLAVGYDTGDTPVN
                     EYLRNISIQLVVQNIMDKHAPFEYRTATGGGNPCACDILKNLFGRQTQIRIVKTW"
     gene            complement(12479..12829)
                     /locus_tag="VGM72_11205"
     CDS             complement(12479..12829)
                     /locus_tag="VGM72_11205"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY4265875.1"
                     /translation="MALRLRFSIALGLIALSASAAAGQEGPAGNAQNGRQLFQAKGCY
                     SCHGFVGQGSREGPRLTPPLALPALIQQLRTPRAIMPPYEAALVTDAQAADIHAYLAG
                     LPKPPDPKTVKLLQ"
     gene            complement(12835..>14245)
                     /locus_tag="VGM72_11210"
     CDS             complement(12835..>14245)
                     /locus_tag="VGM72_11210"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY4265876.1"
                     /translation="VEWNHAALDQNATVRDFTKWDAQPTSLQATAEALVQAYDLATTA
                     PMGPVLVTIDTDVQEDPLSKEEEAKLRIPKLTRRAHPQGDDESLREAAKLLVAAENPV
                     IYANRYGRTENAPALLIQLAELLHAPVIDGRARMNFPSRHKYNHSSRREQTLAQADCL
                     LALEPVELWGLTNDVQDLIGRPNRRLNRQANLKIIHIGTESLLLKANYGDVQRYAPAD
                     LAIAGDAEASLPSLIQAVKRELTPARSAALAQRGEKLAAMTPRLLEQARADAVYGWDA
                     SPISVARTCMELWDQLKNEDWTMPTETVFLSDYPHRLWNIDKPHNWIGGSGAQGVGYN
                     APAALGAALANKGTGRVTAAIVGDGEMMMTNTMLWTAAHHQIPILAIVHNNRAYHMEV
                     MHTLRMADRRSRDTSRIHIGTDITNPNIDFAALAKSMGVYGQGPIENPNDLGPAIKRA
                     IDVVKRGEPALIDVVSQPR"
BASE COUNT         2562 a         4257 c         4437 g         2989 t
ORIGIN      
        1 tccgcgaatt tgccgatcgg cgaggccacg tcattttcct tcgacatgcg gcgcaatccc
       61 gaagacggca tcgtgccgtt ccagaccctt gccggtcctc aggaccatct gacccatgtc
      121 acggtcatgc gcatctcagg cgtggccgag ggcatcggtt ggacggcggg aaacggtttg
      181 tcgttgcatg acggcatgat gggcgatggc ggcagcccct tcgcgggcgc gtcgctgacc
      241 aatgcgtttt cgccgcttgt gagggcggca cccggcgcca tggcgagttt ccgcctgcct
      301 ttgtctccgt caaccgggct ttccttcgga gcggggtttt ccgaaaatca gggtctcacc
      361 gatcatctgc gcaccccttt ccgcaacagc gcgcagagcg cggaaatgcg gctcgatcat
      421 gtggcgggac agacccgctt cagtctcgag gccggcgatg tgctggaaac cggcggcttc
      481 atgggttcgc tcgccgcggg cggactgacg atggcggcac aggcctccac cgtctggacc
      541 acggcgtcgg ccgaagtgac gctcgatgcg cattggtcgc tcaaaggcgc cctgacactg
      601 gccgcgaccg ccgcctctca cactcaagcg agtctcatca ccgccatcgg cccggtctat
      661 gccacgagtt tcgcgctcgg cctggcagga gagaacctct tccgcggcgg cgatgtgctg
      721 agcttcacgc tgggtcagcc tttgcgcgcc gaacgcggca cgctgacgct ggtctccggc
      781 gtctcgcggg actggaccga tggcggaatg atcatgggtg agacccaggc ctcgctcgaa
      841 ccttcggggc gcgaattcga tctggaaacc ggctaccgtt tcgaccttag aggctggggg
      901 ttcggcgcca atgtcgccta tgccatcgac cccaatcatg tgcagaacgc cagcgccgtc
      961 gttgctctct tcaccctgac gcgcgggttc tgacgcgcgg caagttcagc ccgcggctgg
     1021 attgtagtcg gaaccgccaa gcacgatgtt gatcaccttt tcctcggtgt agctcaaaag
     1081 ctcgctgatg ccttcttcgc gcccgatgcc ggaattcttg aaaccgccat agggcacgcc
     1141 ccgataatgc tgacccacgc cgttcaccca gacatagccg gcctgcacct gttgggcgac
     1201 gcgcagcgcg cggttgatgt cgttggtcca taccgcggcg gtgaggccta actcgatggc
     1261 gttggccatt tggagcgcct ccgtttctct gccgaatttg aagatcgaca tcaccggtcc
     1321 gaacacttct tccctggcga ggcgcatgct catgtccatc ccgccgaaca cggtaggttc
     1381 cacccaatag ccgcgctgga attcctcacc cttgggaatg ccgccgccat gaagcaattt
     1441 gccgccttcc ttatgaccga gatcgatata agcgagtacc ttggcgtact gcgccttgtt
     1501 gttgatcggc cccatattgg tcgcgggatc gagcggatgg cctatcctga tcttgcgcac
     1561 ccgctccagg attttttcca ccacgcgatc atgaatggat tcatgtagca ggatgcggct
     1621 catggaaccg caggattgtc cctgccagcc gaaattcatt ccgcctacgg agcaggccgc
     1681 aaccttgtcg agatcggcat cttcgaaggc gatcagcgga ttcttgccgc ccaattcgag
     1741 ggtaacgagt ttcacggcgg tttcggcggc tgccttctgg atcgccatgc ccgaggcggg
     1801 ctggccgatg aaggcgatgc gtttgacgcg gggatggcgc accagcgcct cgccggtttc
     1861 acgcgcgccc gtcacgatgt tgacgacgcc cggcggcaac acttccttgc agatttcacc
     1921 caggatcgag gccgacaatg ggctctgctc ggagggcttc accactatcg cattgcccga
     1981 aatgatcgca ggcgcgatgc gcgaggcggc aaaaccgatc ggatggttga acggtatgat
     2041 gcggccgatc acgccataag gctggcggat cgtcatatgg atatttcccg acgttgcggg
     2101 aatggtttcg cccttgatct cgtaggcgag accggcggcg aaacgcatgc gttcgaccgc
     2161 cgtgcgcacg tcgcgcttca tgggaccgag cgtattgccg gtgtcgagcg attcaatacg
     2221 ggcgatttca tcgccccgct tgaggatcgc atcgcccagt ttgtgaatgt attcggcgcg
     2281 ctggcccatg gtgagcgcgg cccaggcggg ctgcgctgct tcggctgccg cgaccgccgc
     2341 atccatatcg gccttcgtgc ccatcggcac gcggcccaat tgggtttcat cagcggggtt
     2401 gatcgattcg agccagccgc catccgcagc ctcgatcagt tcgccgccga tcagcatatg
     2461 gcccgtcatg taggccatgg tcgcgttggt cccgtgcatc ggcctctcct tcgaggtcag
     2521 attttttctg gtgagcgacc ttagagtatg gctttgcctg tttgaagtga catcaggcgc
     2581 ccgcgacatc cagcgttgcg atctcatcgg ggaaaagttt cagcgccgcc gccgcggcga
     2641 aatcgccaag ctgttcgatg ctggtcgcac tcgcgatggg cgcggtgatc gatggccgtg
     2701 cgatcagcca ggccaatgcg atcgcggccg gtttcacatg atgggccgcc gccaccgcat
     2761 cgagcgcggt taggattttc tttccttttg cattcaaata attggacatg cctccgccgc
     2821 gcgggctttt gccgaaatcg accgcgctgc gatatttgcc ggtcagaaag cccgccgcca
     2881 agccgtaata tggaatgacg ccgatattct ctttcagaca aagctgttcc agcgcacctt
     2941 cgtaatcgtc ccggtcgtag agattgaagg gcggctgcag gcactcataa cgcggcagac
     3001 cgtgcttcgc cgaaacgtcc agggcttcgc gcatgcgtgc cgcgctgaaa ttggaggcgc
     3061 cgatggcgcg aaccttccct tcgcggatca gctccgcata ggtttcgagc gtctccagca
     3121 tcggcgtgga gatgtcgtcg cggtgcgact gataaagatc gatggtgtcg gtctgcaagc
     3181 gccgtaaact gtcctcgatt tccgccttga tatagcggcg cgagagaccc tttttgtccg
     3241 gccccatttc agcgccgagc ttggtgagga tgagcacgcg ttcgcgccgg cccccgccct
     3301 gtttcagcca attgccgata atggtttcgg attctccacc cttgttgccg ggaatccagc
     3361 gcgaataggt atcggcagtg tcgagggcgt tgaaccccgc atcgacgaag gcatcgagga
     3421 ttcgaaatgc cgatgcctga tccacagtcc agccaagcac gttgccgccg aacacgaccg
     3481 gagcaatctc caacgcggat cggccaagct tgcgtttttc catggtcgcg accttcccat
     3541 atccgcttct ttcttaaagt ggcatccgat gagggtcaaa agcttgccaa gccgccatag
     3601 gtatgatctt tgccatcaaa catatgatcg ccacctcacg cttttccctt ccccggatcg
     3661 tcggcatttt cgccgtgctg atcggcgcca atatcgccgt ctggatatgg gcgatcgcgg
     3721 ccttccagca ttaccccgta ctcctgggaa ccgcgcttct ggcctacagt ttcggtctca
     3781 ggcacgcggt cgatgccgat cacatcgcgg ccatcgacaa tgtcacgcgc aaattgatgc
     3841 agagcggcaa gccgtccgcg agtgtgggcc tgttcttttc actcggtcac tcgacggtgg
     3901 ttatactcgc ctcgcttctg atcgcactcg gcgcgggtgc gctgaatgcg cgctggtccg
     3961 cgttcaggga tgtcggcgga ttgatcggca cgtccgtatc ggtgttgttc ctgttcgcca
     4021 tcgcgattgc caatctgttc atcctcgccg gagttttccg cacatttcag tatgtgaagc
     4081 gcggcggacg tctggccgaa gacgacggcg ggatgctgtt cgccgggggc ggcctgctgg
     4141 cgcgaatttt cagaccgctg ttcggtttca ttcgcgcgag ctggcagatg tatccgctgg
     4201 gtgtcctgtt tggactgggc ttcgacacgg caaccgagat cgggctgctc ggcatttccg
     4261 cggcccaagc cgcccgcggc ctgtcgatcc tgccgcttct ggtgttcccg gcgctcttca
     4321 ccgccggcat ggcactggtc gacaccgccg attcggtttt gatgacgcgc gcttatggct
     4381 gggcattcgt gaagcccatc cgcaagctct attacaacct caccatcacg gcggtttcgg
     4441 tgctggtcgc ggtgcttgtg ggatcggtcg aagccttggg actgattgcg gacagactcg
     4501 cgttgaaggg ggcgttctgg gatgggatcg aagcgctcaa cagccaactc ggcgtgctcg
     4561 gctatctcat catcgcgctg ttcgccgtga gctggatcgt gtcggtgctg atctatcgcc
     4621 tgaaaggcta tgacgggctc gatattgccg gcgagactcc ctgaaattac ccgtcaatgc
     4681 ggatcggcgc cgctttattg gagctgtttg atcgatttcg gatcgggccc tttcgggatg
     4741 ctggaaagat acgcgaaaat atcggcgaca ccggcatcgg aaatctgact ggccgtatag
     4801 atcggcatct cgtaacgggg attgcgcaat tgcgcgagca ccgcgggaaa acccaatgcc
     4861 gcggcgttga gatgcggccc gtcgcgcagc gtgccctgcc cgataatgcc gtgacaggta
     4921 taacagccga gttttttgaa attcaccagt cccgctttgg gatcgcctct gggcgtgtcc
     4981 tgggcttgaa ctgcgccaaa cgagagaacg gcaagcacca cgccaagcgt caaactgcgt
     5041 atcggcatcg ccattaccgt ccgtccgaca cgatgtcgat gagcgcgggt tcgcccttct
     5101 tcaccacatc gatggcgcgc cggatcgccg ccgtcagatc tttcgggtcc gacaccggcc
     5161 cttgcgcata aaggccatag ccttgcgcga tcttggcgta atcgggcgcc ggatcgtcga
     5221 tcagcgtgcc gacatagaca ttcttgttgt tcacccgctg gcggcgcgag gccatgcgca
     5281 ggatcatcat cacttcctga tgataggcgc gattgttgtg caccagatac aggatcggga
     5341 ttttgtgatg cgccgcggtc cacaagacgc ccggcgccat attgagatcg ccgtcgccga
     5401 ccatagccac cgtcagccgt cccttgtcct tgttggcaag tgcggcaccc agcgcggcgg
     5461 gtgaattgta gccgatgcct tgcgcgcccg acccgcccat tgtgcgatac ggcttgtcga
     5521 tgtcccaaag acgatacggc caatcgctga ggaattgcgt ctcggtgggc atcatccagt
     5581 cttcgttttt gatctggtcc cacaactcca tggtgagccg tgccgatgag atcggctggg
     5641 tctcccactg atacgccgct tcctgacggg attgatcgag cagcttggcc gtttgcgcct
     5701 tcaccttggc gcagcggccg tcgcaacggt tcttgtcggc caccgacatc tgcgttttca
     5761 gggcttcgat cagcgcaggc attgtggctt cggcatcgcc cgcgatggaa aggtcggccg
     5821 cggcgaaacg ctgaaagtcc tggaaattcg atttcgccat catcccttcg gtgccgatat
     5881 ggatgatctt gagattggct tggcggcggc ggggatcgtt gggacgcgtc accaggtctg
     5941 gcacgtaatt gttgagtccg aaaagatcga tgggttccag ggcgaggatc acatcggccg
     6001 actgcaaagc cgcctcgcgc cgcaaggaat gattcagcgg atgccggttg ggcatgttca
     6061 tgcgattgtg gctgtccaca accggcgcgc caatgagttc ggcaagctgg atcagcagag
     6121 cgggcgcgtt ctccgtgcgt ccatatcgat tggcgaagat gacggggctg tccgccgcga
     6181 tcagcatctt ggcgacttcg cgcacggccg cgatatcgcc cgccggctgg aagcgcatct
     6241 tcagcttcgg gatatggagc ttcttctcga cttcggcggg aataggatct tcctgcagat
     6301 cgccatcggc gacgatgagc acgggcgcca tcggcgccgt cgtcatcatg tcgtaggcgc
     6361 gcaccgtgga ttcggcaaag ccctgcagcg aggcaggctg gtcgtcccat ttggtgaagt
     6421 cccgcaccat ggcgccttgg tcctgcgcgg catgatccca ttcgatgtag gcctgacggg
     6481 tttcctgctt accgatattg ccggtcagga ccagcatcgg aacgcggtcg gcgaaggcgt
     6541 tgtacatcgc catggtggcg tgctgcaggc cgaccgtgcc gtgcaccatg caggccatcg
     6601 gcttcttcgc caccttgtaa tagccgtggc acatggcggc cgcagtgtct tcatgcagcg
     6661 tggtgatgaa ttccggcttc ttgttgtcgc cgtaattgat gatggattca tgcaagcccc
     6721 ggaacgtcga tcccggacaa gaggcgatat aatcgatgtc gaggctcttg agcacgtcga
     6781 ccatgaaatc cgatccggtc ttgccgatgg tctgcggcgg cgtgtcgctg ctgggccgat
     6841 cggtcatggg caccgggggc acactggaca ccggtgccgg cgtggtctgc gccaccgcct
     6901 tgcccgcggt cgcggcgagc ccggcggccg gtgcggcagc catccccgcg aggaactggc
     6961 ggcgcttgat cgttttggac atgaaggcct cccgctaggg catgcacgcg cgcacgcggg
     7021 tgcccgaatt tgccattgcg cgagtttgcc attgggcatg gccaattcca agccgttcac
     7081 ggggaattgc cgttaaccag ccctgcacgc caagctagtc gatgagatcg atcccgatct
     7141 gtgagcgatc atggcggaaa aggaccggca ccacatcgcc gggcgcaacc gtaacaggcc
     7201 tcggaaagcg gtgcaggatg tgggtccaat gaccgttgaa cggagcattt ggcgaaggcc
     7261 ggttctcata ggtcgcatgg gcgtcgagtt ccagcctgat ccattgcgcc acgcccacgc
     7321 agatgccgtg ttgcgtggcc ttgagcgcaa gccgcacccc gcccatcgga aaacgctttt
     7381 cgcgaaaatc gaaacgcatc acctcgacat cgcccgacaa aacacggtgg ggaatattgc
     7441 cgagcgccac cgcgagcatg gcgggcgcga aatcgttgaa cccggaaaga tcgaagcccg
     7501 caatcctgcc ggcgaacagc atgccctgga gcgtttctcc accggcaaga tagcccatga
     7561 tcgaggccgc cgccggaatg atcgcagcat cgggcaccag cagatattca tgcgcgtgct
     7621 cgatcgcagg caatacgcct tcggtgatga gaccgctggc aaaggtctcc gtcaccagca
     7681 cctcggcgcg ctccggcatg atgccgggaa cggacatggc ggtcgatttc agcggcacga
     7741 cgccgatgcg atccgacaga ccgtttctga cgatgatctc gcgcgcccgc tcggcaatca
     7801 gcgacaccgc ctcgcaggtg gtgaccttgg acgcgcccgc ccgcgcggcc atcagcgcga
     7861 gaaggccggc gcccgttccg atatcgagga cccttttgcc cggcgccgca cggcggatcg
     7921 cggcctcata agcgccgttg cgtttgtgat cgtccatcat ggcaaaatgc caactcggca
     7981 ccacgccttg ataggtcgcc ctgagattgg cggtggcgtg caacaggccg ggttcgagca
     8041 ccacggcttt gcgaaaagca ctgacggcct gctcgggctt ctgcgtctca agatagatca
     8101 cgccgagatt gttgagcgca tcggcgtagg cggggttcat cgccagcgcg cgttcgaacg
     8161 cggcgaccgc ctcgtccttg cggccgagct tgctgagcac ggcgcccaga ttgttgtgca
     8221 gctccgcagt ggcgcgcggc gatgccgcgg cccgtttcag gaaggcgagg gattcttcga
     8281 tccggccctg ctgaaagcgg atggcgccca gaagatgatt ggcttccgcg tgggcggggt
     8341 tggcgtcgag caaatcgcag gcacgttctt cggctgcatc gaaatgcccg gatttgaaat
     8401 gctcataggc ctgggacagc gaatcggttt gcatctagcg tgaatttgct tcgtggaatt
     8461 tgaaccggga ttgccggtgc cgtcccacat ctggggtgcg ggagatcgat agcgttttgc
     8521 gcaaacaaag catggccgcg ccagcctttg tcttccgagg ccatcataag ggacgcgcgc
     8581 gattggcgac atcgtttttg cgcagggagc gttcccactc tccgcgcaaa aacgagactc
     8641 tttcctacca ggtcttcacg attctgatct gggtctgccg cccgaagagg ttcttcagaa
     8701 tatcgcacgc gcaaggattg ccaccgccgg tcgcggttcg gtattcgaac ggcgcgtgct
     8761 tatccatgat gttctgcacg acgagctgaa tactgatgtt tctgagatat tcattcaccg
     8821 gtgtgtcgcc ggtatcgtat cccaccgcca gatcgaaggt gtaataggac ggttcgatgt
     8881 tggtgtaatt gttgagcgcg caggggaatg tgcccccgcc gaccgagccg cctgccgtga
     8941 gacattcgaa gttgacgttc ggcggcgcgg tttgcgtatg gtagaaatgc ccctggtagt
     9001 ccatgaaccc ggtgacgctc cacggcccgt tgctccagcc gagccgcgcg cgatatttga
     9061 atcgcggcag cgattccaca ccgagctggg gcaccccgcc gaccgaggcc aggtcggtgt
     9121 cgtacagaac gtcggtcacc tggcccgcgg gacccgccgc gcccggaacc ctgaccgatt
     9181 cctgcttgag gtaataggtg ccggtcatac ccgtgttcca ggcgccgaga tcgcccaaat
     9241 cccagtcata gctgatggac cagtctatgc cttcggtctt ctgccagccc ttgttcaacg
     9301 tgccgccgtc attgatccaa tagatcaggc tctgggccgc ggacgggaca ggatcggtgg
     9361 gatgcgcaag cgctcgctgc accatgtcct ggaaggccgc gcactgctcc ggatgcgcat
     9421 tctgcgccac cgggcagcca acatcgctcg gcaccagata gacgaacgcg agcgaggaat
     9481 cgttgaatcg gttcgttgtc gggttgccga aattgatcag gatgccgttg atcttgaccg
     9541 tgtaccaagt tgcctggaca tcgagtcccc tgaggaagct ggtcggtgcg aaatcgaatc
     9601 cgaatcccca attggtcgac agttcgggac gcaggacctg gttgtcctga ttgaaatagg
     9661 tgcggaaatg cgcatcgacc gcagccttgc cgccgccgtt gagcgaaaga cccgcaggct
     9721 gcgaattgca ggcaaatccg gcattgaaga gcttttccgc gccggacccg gccggaggct
     9781 tgccgctggc gtcgcaagcg acctggatgg ttgcgttgtt ctggaattgg gcagccccgg
     9841 gcgcattctg caatccccag ccgttccacg caacgttgga gatcggcgag aactcgccga
     9901 agctgggagc gcgaaacgat gtgccccacg agccgcgaaa tgtcaggccg agatcttcgg
     9961 agacttccca gttgaaggcc agcttcggat tgctggtgcc gccgacatcg ctgtactggt
    10021 catgccgcca ggacgcttcc agttccagtt tgcgcacacc cggaagcgag ttgttgtccc
    10081 cgatgacggg aacgttcaac tgtccgaata ccgcccagac gcggcgcgat tccggatcgc
    10141 gcagcagggt catcgcggtg ttggatgtgc tggtctccgt gttgctctgg atgaggaaac
    10201 gatagctggt gtagttggcg ccgaccgcca ttttcaccgg cccgcccggc agatcgaaca
    10261 atgggccatc cgccttgaag cccttctcat cgacccagaa ggcttcggcc gtttcattgt
    10321 aggccccgat aaaggccaat gtgctggggg aattgcactg gtacgtcgtc gtgtcgcaga
    10381 acagattcag atagggcgtc gtcggcggtt tggtgaacgt cccgatggtg ggatttgtcc
    10441 cggtggccgg cgttgccggc atcgtccagc ccagagccgc tgaaaccgcc gcccggttca
    10501 ccgcgccgac gacgtggttg tactctgcgt cccgcgtctg ggaataatag acttgcgccg
    10561 cccagtcgcc cggcagatcg atgttcaacc cgcccagata gcgttgcgcg gatgcgtagg
    10621 aattggtgat cgaaggcgat tcgatcgaaa aattgtagct gacgcgcagg ccgttcggcg
    10681 cgttggtcgg gtaataagga ttgaatgtgg gcaccgcaag cgtgagctga ttgccgacgg
    10741 tattgttgat gaatcgcgaa cggcgcatgc catagaagcc ttcgccgtag aagctgatgt
    10801 ccttggtcaa gcgctgatcg aacgtcaccg agctgccgac atattgggag gccgcgctgt
    10861 aatcggtgat cgaataggga ttgaattcat tggccgtgcc gttgacggga ccgccattgg
    10921 cgggatttgc aaacgtcgcc caattgatcg tcgccgccgt cgagccgcca agcggaccga
    10981 ggccgccgtt gatggtattg aaatccctgc ccgtcccttg ggggatcgag aagcaattcg
    11041 tgcaattgtg accgttggtg gcgggataat tcgtctgatc cggcgcggcc ggcgcgcccg
    11101 tggaaatgat gccgggcatc gcggaagcga gcggcgtgcg attgtccaat ccccacgggg
    11161 tgaagtcgaa tgtgtatttg gaattgaaat tcccgtgcgt cggggcgacg tcgtaccact
    11221 gataaccaat cgtgatgtcg ccgccgtccc aggtgcggcc ccacaattgc gaggcgagat
    11281 atttggtatt gccgcctgcg cccgtcgtga acccgatttc ggtctgggcg ccgtcataac
    11341 cgcgtttgag aatgatgttg atcacaccgc cgatggcatc tgagccatag gtcgccgaag
    11401 cgccgtccaa caacaggtcg attcggtcga ccgcgacggt cgggataatc gatggatcga
    11461 tctggcaaag tcccatgcct tgcgccgggt agcgcacgcc gtcgatcatc atcagggaac
    11521 gcggcgcaga gccggtatcg agctgacgga gattgacgcg cgttccgcgc tcgacattgg
    11581 cggcctgtgt cccgaccggg cccggaatga cgttgaactg ggggatggtg cggaacaggt
    11641 ccgatgtggt gagcgaaccc gtcatgcgga tgtcttggac gctgagattg gtcaccggga
    11701 caccgaccgc agcggtaccg cgaatgagag agccggtgat cagcacctgc tcggggactt
    11761 ctccgggggc ggcctgtgcg gtttgggcct gggcggtttg ggcctgagca acctgggctt
    11821 gggcaacctg gacctgggca agctggatct gcgccggttg ctgcggcgcc acctgggcct
    11881 gcgcaatgcg agacggtgtc acctgaacct gtgctatctg cggttggtct accggggatt
    11941 ggtcagtttc ggcttgtgcc gccagcgaat tggctaaggt cagaaacgag gctgtaccca
    12001 acagccttgt gagtctggac gtcgtcggat tcatcgcaag tcctcccctg ggcaggtgac
    12061 ctaccctcgt tttgtttggg cggaattcgg acgtctcagc tgttacgaat taccaggcta
    12121 caaattccat ttcctcattc cgccttatcg atgctcagac gcgactctca gctgatggat
    12181 gaaattcggt taacttaatt acagacagag gcaaggaaag ccgtcaacga aacaagcgcg
    12241 tgctggcccc aaatgccacc attattctgt tgttgttgta atgaagatac agaaaatgat
    12301 gtttgttggc gtataaacgt gctcgatggt ttagtttttg atcgaatata acttaaacca
    12361 tgatttccgt ttccggggtc gcgccgcgca gaaacgcgaa tcatccaggc ccggtgattg
    12421 cggcctagtt gcaaaatttt taatcagcgt cgaaaaccgg gaagttccta tgcgcccgtc
    12481 actgaagcag cttgaccgtc ttggggtcgg gcggcttcgg caatcccgcc agataggcat
    12541 ggatgtcggc ggcttgggca tcggtgacca gcgccgcctc gtaaggcggc atgatcgcac
    12601 gcggtgtgcg caattgctgg atgagggcgg gcaatgccaa aggcggcgtc agacgaggcc
    12661 cctcgcggct cccctggccg acaaagccat ggcaggaata gcagcccttg gcctggaaca
    12721 gctgacgccc attttgggcg ttgccggccg gaccctcctg acctgccgca gcgctggccg
    12781 atagcgcaat caacccgagc gcgatggaga aacgcaatct cagcgccatc gtcttcatcg
    12841 tggctgggac acaacgtcga tcaaggcggg ctcgccgcgc ttgaccacat cgatggcacg
    12901 tttgatggcc ggtcccagat cgttgggatt ttcaatcggc ccctggccat agacgcccat
    12961 ggatttggcg agcgcggcga aatcgatgtt cggatttgtg atgtcggtcc cgatatggat
    13021 gcggctggta tcgcgcgagc gccggtcggc catgcgcaat gtgtgcatga cctccatgtg
    13081 ataggcgcga ttgttgtgca cgatcgccag gatcgggatt tgatgatggg ccgcggtcca
    13141 tagcatcgtg ttggtcatca tcatttcgcc gtcgccgacg atcgcagccg tgacgcgtcc
    13201 cgtgcctttg ttggcgagcg ccgccccgag tgcggccggt gcgttgtagc ccacgccttg
    13261 cgcgccggag ccgccgatcc aattgtgcgg cttgtcgata ttccacagcc gatgcggata
    13321 atcggagagg aacaccgttt cggtcggcat ggtccagtct tcatttttga gctggtccca
    13381 caattccatg caggtgcgcg cgaccgagat cggtgaggca tcccagccat agacggcatc
    13441 ggcgcgcgcc tgctccagaa ggcgcggcgt catggcggcc agcttttcac cgcgttgcgc
    13501 aagcgccgcg ctccgtgcgg gcgtcagctc gcgcttcacc gcctggatga gcgagggcag
    13561 gctcgcttcg gcatcgcccg cgatagcgag gtcggccggc gcgtaacgct gcacatcgcc
    13621 ataattggct ttgagcagca ggctctccgt gccgatatgg atgatcttca gattggcctg
    13681 acggttcaat cgccggttgg gccgccctat cagatcctgc acgtcattgg tgaggcccca
    13741 gagctccacc ggttcgagcg ccagaagaca atcggcctgt gccagcgtct gctcgcggcg
    13801 gctggaatga ttgtatttgt ggcggctggg aaaattcatc cgcgcgcgac cgtcgatgac
    13861 gggggcatgc agcagctcgg ccaactggat cagaagggcc ggtgcgtttt cggtgcggcc
    13921 ataacgattg gcatagatga cgggattttc cgccgccacc agaagcttcg cggcctcgcg
    13981 aagcgattcg tcgtcgcctt gcggatgcgc gcggcgcgtc agtttcggaa tgcgcagctt
    14041 ggcctcttcc tccttgctga gcgggtcttc ctgcacatcg gtatcgatgg tcaccagcac
    14101 cggacccatc ggcgcggtcg tcgcaagatc ataggcctgc accagggctt cggcggtcgc
    14161 ctgcagcgag gtgggctgtg catcccattt ggtgaaatcg cgcacggtgg cgttctgatc
    14221 gagcgcggcg tgattccatt cgacc
//
LOCUS       DASYEG010000260         4097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2413647, whole genome shotgun sequence.
ACCESSION   DASYEG010000260 DASYEG010000000
VERSION     DASYEG010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2413647"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..1351
                     /gene="lptF"
                     /locus_tag="VGM72_11215"
     CDS             197..1351
                     /gene="lptF"
                     /locus_tag="VGM72_11215"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEY4265877.1"
                     /translation="MPANARLPRLSNYVLRQLAGPIALFCFLLTGVVWLSQSLRLLDL
                     VINRGQSAPTFIYLTLLVLPSLLVIILPIGFFAGALYGLHKLNADSELVVMQACGYSR
                     VQLGIPVMISAIVVMAATYLCSLYLMPLGQRTMRSAVLDIRADIGAAMLSEGAFNTPA
                     DGLTVFIRELAPDGHIRGILVHDNRDARQPTTYIAESGVLAQTSGGGRLIMQDGTIEQ
                     ANKAGAALSVLKFKRYVFDLDQFSSQQQNTQLEASDRYLNELFWPQFKTPPKNRNILN
                     IFLAEGNNRLAAPLYCIAFALLALAAVTSGRRQRGAYALRLVLASVFAAVLRIVGYGA
                     QSAAARNPSLLFLLYLVPIMGGALALIDLQGTILPRLLERLRGPAPMAPA"
     gene            1348..2439
                     /gene="lptG"
                     /locus_tag="VGM72_11220"
     CDS             1348..2439
                     /gene="lptG"
                     /locus_tag="VGM72_11220"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEY4265878.1"
                     /translation="MTWSWTLFRYLARQFIVGVGTVYAGFLFLALSIDIVGLADHTAE
                     KHVPTGVVIVMALLQLPDLGLKLLAFAVLLGGVFAFVRLSRSQELIAIRAAGVSAWDL
                     LAPSLAVAVALGVFTVLALTPLTSRMLVQYSEMEAKYIRGQASQLAISRNGLWLRQGD
                     ARSQSVIHALRVANQGLRLEDVIVFRYGENDRFLGRIDARSAVLETGAWTMRDAYVSG
                     PEGKTVHHPAYSFATTLTPAQIQESFASPSTISFWDLPYFIATARKAGFSAVRYVLYF
                     DSLLVMPALFAAMVFMAASFSLRLARMGGLLRVILYSALAGFGVYFFGNVTEALGESG
                     ILPTALAASAPAAAAILLGMTLVFHQEDG"
     gene            2439..>4097
                     /gene="lptD"
                     /locus_tag="VGM72_11225"
     CDS             2439..>4097
                     /gene="lptD"
                     /locus_tag="VGM72_11225"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEY4265879.1"
                     /translation="MRHTKFLGLLTAALMMSGASVHAAGTIGKNALLGPSGGDYVLKA
                     DDVTYDVKAKVVVATGHVEVDHNGRIVTANQLTYNQDTDTVVASGNVVMMAPDGNVIF
                     TPRATLSNEMKDGVLEGFQALIGKTGRLSAATATRKDGTVTTMERAVFTHCKICNKPG
                     QRTPLWDVKAYHVVYDQTAHKITYRDAVIDMFGIPVAYTPYFSQSDPTVKRETGLLAP
                     DIGSSSTLGSFVKLPYYISFNDSQDMTIEPIVSTEGGEVLEGEYRQRWNNGGMWLQAT
                     IANNPEGGINEHENVWYSSFFGAGRTPIGDNNVWRFGFDAQLTSNPTYLERYSLYNKD
                     DQLFNDLFVEGISGRSRFAVTGYFFQSLVGSCPQLSSTKPPVCATTQISPLFIKTAQI
                     PLALPLIEYSYIPERTVLGGDFRFDLNSVSLERNLGPDDQRLTGEMRWQLPFVTGNGQ
                     LITIRADARGDIYRVTNNDPIDFPDIPTKTNYITRGIPYLGIDWRWPFVTGGGAGNTA
                     FIVEPIVQAIAAPYGGNPKGIPNEGSLNIELSENDVFSFDRIPGY"
BASE COUNT          746 a         1316 c         1191 g          844 t
ORIGIN      
        1 gggcggcaaa ggaaagctgc atggggatac ctccgggtaa tgtttgcggc cctttaatcc
       61 aaaaaaatgc gctccgtcag tcggatgcgc gatttccggt tgccgggtgg cggcgggaaa
      121 atgctagtta gtgctagatt cagcttaggt cgaatccggg gccgggacca agcgccgcgc
      181 cagggctttc aaggtaatgc cagcaaacgc aagactgccg cgattgtcga actatgtcct
      241 gcgccagctc gcgggaccga tcgcgctttt ctgcttcctg ctgaccggtg tggtgtggct
      301 ttcccaatcg ctgcgtctgc tggatctggt catcaaccgg ggacagtccg cgccgacgtt
      361 catctacctg accctgctgg tgctgccgag cctgctcgtc atcatcctgc ctatcggctt
      421 cttcgccgga gcactctacg gtttgcacaa gctgaatgcc gacagtgaac tcgttgtcat
      481 gcaggcctgc ggctacagcc gcgtgcaact cggcattccg gtgatgattt ccgccatcgt
      541 ggtgatggcc gccacctatc tgtgcagcct ctacctgatg ccgctcggcc agcgcacaat
      601 gcgcagcgcg gtactcgaca ttcgcgcgga catcggggcc gcgatgctca gcgaaggtgc
      661 attcaacacg cccgccgatg gattgaccgt gttcatccgc gagctggcgc cggacggtca
      721 cattcgcggc atcctggtcc acgacaatcg cgatgcgcgc cagcccacaa cctatatcgc
      781 ggagagtggc gtcctcgccc agacatcggg cggcggacgc ctcatcatgc aggatgggac
      841 aatcgaacag gcgaacaaag ccggcgccgc gctttccgtc ctgaagttca aacgctacgt
      901 gttcgatctc gatcagttct caagtcagca gcagaacacg cagcttgaag ccagcgatcg
      961 ctatctgaac gaattgttct ggccgcaatt caagacaccg cccaagaatc gaaacattct
     1021 caatatcttc ctcgcggaag gtaacaaccg gctggccgcg ccgctctact gcatcgcgtt
     1081 cgcgctgctc gcgctggcgg cggtgacgag cgggcggcgg caacgtggcg cttacgcatt
     1141 gcgccttgtg cttgcctcgg tattcgccgc ggttcttcgc attgttggtt atggcgcgca
     1201 gagcgcggcg gcgcgaaacc cgtcgctgct tttcctgctc tatcttgttc cgattatggg
     1261 cggcgcgctc gcgctgatcg atctgcaagg tacgatcctg ccgcgcctcc ttgaacgtct
     1321 gcgcggccca gcgcccatgg cccccgcatg acgtggtcct ggacactctt ccgctacctg
     1381 gcgcgccaat tcatcgtggg cgtgggaacc gtctatgcgg gcttcctgtt cctcgcgctt
     1441 tccatcgaca tcgtggggct tgccgatcac accgccgaga aacatgtacc gaccggcgtt
     1501 gtgatcgtga tggcgctgct gcagcttccc gatttgggcc tcaagctgct ggcattcgcg
     1561 gtgctgcttg gcggcgtgtt tgccttcgtg cggctttcgc gcagccaaga attgatcgcg
     1621 atccgcgcgg caggcgtttc cgcctgggat ttgctggccc cttccctcgc agtcgccgtg
     1681 gcgctcggcg tgttcaccgt tctggctttg acgccgctga cgtcacgcat gctcgtccaa
     1741 tattccgaga tggaagcgaa atatattcgc ggccaggctt cgcaacttgc catctcgcgc
     1801 aacggccttt ggctgcgcca aggcgatgcg cgttcccaat cggtgatcca tgcactgcgc
     1861 gtggccaatc agggactgcg cctcgaagac gtcattgtct ttcgctatgg cgagaacgat
     1921 cgcttcctcg gccggatcga tgcacgctcg gcggtgctgg agaccggcgc gtggacgatg
     1981 cgggacgctt atgtgagcgg gccggaaggc aagacagttc atcatcccgc ctatagcttt
     2041 gcgacgacac tgacgccggc acagattcag gaaagcttcg cttcgccgtc cacgatctcg
     2101 ttctgggacc tgccctattt catcgcgacc gcgcgcaagg cgggcttctc cgccgttcgc
     2161 tacgttctgt atttcgactc gcttctggtg atgcccgcgc ttttcgcagc aatggtgttc
     2221 atggcggcga gtttctcgct tagattggcg cgcatgggcg gcctgttgcg cgtgatcctt
     2281 tacagcgcgc tcgccggctt cggcgtctat ttcttcggca atgtgacgga ggccttgggc
     2341 gaatccggca ttctgccgac cgcgcttgcg gcctcggcac cagccgctgc cgccattctt
     2401 ctgggcatga cgcttgtctt ccaccaggag gacggctgat gcgccacacg aaattcctcg
     2461 gcctcctgac cgccgctctg atgatgagcg gcgcgtccgt gcacgcggcc ggcactatcg
     2521 gcaagaacgc cctgctcgga ccgtcgggcg gcgactatgt gctgaaagcc gacgacgtca
     2581 cctatgacgt gaaagccaag gtcgtggttg cgaccggtca cgtcgaggtc gatcacaatg
     2641 gccgcatcgt caccgccaat cagctgacct acaatcagga taccgacacg gtcgtcgcca
     2701 gcggcaacgt cgtgatgatg gcgccagacg gaaacgtgat cttcaccccg cgcgccacgc
     2761 tcagcaatga gatgaaagat ggcgtgctgg agggtttcca ggccctgatc ggcaagacgg
     2821 gacggctttc cgcggccacg gcaacgcgca aggacggcac cgtcacgacg atggagcgcg
     2881 cggtcttcac acactgcaaa atctgcaaca agccggggca gcgcacgccg ctgtgggatg
     2941 taaaggccta tcacgtcgtc tatgaccaga cggcgcacaa gatcacctat cgcgatgccg
     3001 tcatcgacat gttcggcatc cctgtcgcct acacgcccta tttcagccaa tccgatccga
     3061 ccgtgaagcg cgagacgggg cttttggcgc ctgacatcgg ctcgtcgagc acactcggtt
     3121 cgttcgtcaa gcttccctat tacatctcat tcaacgattc ccaggatatg acgatcgaac
     3181 cgatcgtctc caccgaaggc ggagaggttc tggaaggcga atatcgccag cgctggaaca
     3241 atggcggcat gtggctgcag gccaccatcg cgaacaatcc cgaaggcggc atcaacgaac
     3301 acgagaatgt ctggtattcg tcgtttttcg gcgccgggcg cacaccgatc ggcgacaaca
     3361 atgtctggcg cttcggcttc gatgcgcagc tgacatccaa cccgacctat ctggagcgct
     3421 acagcctcta taacaaggac gaccagctct tcaacgatct gttcgtcgaa ggcatcagcg
     3481 gccgcagccg cttcgccgtc accggctatt tcttccagag ccttgtcgga tcgtgcccgc
     3541 agctgtcgtc cacaaagccg ccggtctgcg ccacgacgca gatttcgccg cttttcatca
     3601 agaccgcgca gattccgctg gcgctgccgc tgatcgaata cagctacatt ccggaacgca
     3661 cggttctggg cggcgatttc cgcttcgacc tgaacagcgt ttcgctggag cgcaatcttg
     3721 gtcccgacga ccagcgcctc accggcgaga tgcgctggca gcttcccttc gttaccggaa
     3781 acggccagct catcacgatc cgcgccgatg cacgcggcga tatctaccgt gtgaccaaca
     3841 acgatcccat cgacttcccg gacattccga cgaagacgaa ctacatcacg cgcggcattc
     3901 cctatctcgg catcgactgg cgctggccgt tcgtcaccgg tggcggcgcg ggcaacaccg
     3961 cgttcatcgt cgagccgatc gtccaagcca tcgccgcgcc ctatggcggc aacccaaagg
     4021 ggattccgaa cgaaggcagc ctcaacatcg aattgagtga gaacgacgtc ttcagtttcg
     4081 accgcattcc cggctac
//
LOCUS       DASYEG010000261        14544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_5170340, whole genome shotgun sequence.
ACCESSION   DASYEG010000261 DASYEG010000000
VERSION     DASYEG010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 14544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14544
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5170340"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VGM72_11230"
     CDS             complement(<1..385)
                     /locus_tag="VGM72_11230"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4265880.1"
                     /translation="MPDSLQAAATSLNRVGGASETANRHQDSHQDKWAAQPPDFEALY
                     GLARNRLKNGELDEALLTFRRALALRPRFAEGWRLQSAVLRRLQRWQDALACIDTALS
                     IEPGSAEALSSRAAILAEASRPAESL"
     gene            complement(583..2397)
                     /locus_tag="VGM72_11235"
     CDS             complement(583..2397)
                     /locus_tag="VGM72_11235"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing
                     glycosyltransferase family protein"
                     /protein_id="HEY4265881.1"
                     /translation="MLKAGHTHFGAHYLLGIIALQRGQFQIAEQRIAGALALNPNAAS
                     AHRDRAVALARLDRNAEALASLDRAILLKPDDADAHGHRGNVLQELGRFADALECYDR
                     AIALSPLSPILHHNRGIARHSLGQYEEALAAQDRAIALKPDYAAAFNKRGTVLWALER
                     HDEALASYDAAIARTSDFVDAHYNRGLALQHLGRFGEAVESYDRALAFAPDFAEALSN
                     RGNALQKLGHLEAALESYDRALAIKPDFAEAHYNRGVMLLALKRPEEALASYDRALAR
                     APDHPEILFSRGVCKLALGRMERSAWDDYENRWRMRNYPARGSGVDAPHWTGEDLAGC
                     SILVYAEQGLGDIIQFSRLVPLLGSRGADVSFLVPAKLHRLLSGLPGDMRLLAALPDR
                     GFDFQCDLQSLPRGLGLEFGDIPLDIPYLAVEPSRVDHWRKTIGPHGFRAGIAWQGAR
                     WHNGVEIVGRGVPLREFYPLSQIPGLRLIGIQKNEGAEQLADLPAGMQVETLGADFDA
                     GPDAFADTAAAMQHFDVIITCDTSIAHLAGALGRPTWIALKYVPEWRWMLNGSDSPWY
                     PSARLFRQKIDKDWSAPIAGMVERLRQSLAARPSGRQE"
     gene            complement(2498..3388)
                     /locus_tag="VGM72_11240"
     CDS             complement(2498..3388)
                     /locus_tag="VGM72_11240"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEY4265882.1"
                     /translation="MARPRGQETARLTMRLELIESLTLPGDATKPNEDAYAFSPVAAA
                     VFDGATGLGEQLMPGKSDAQWLAQFGARRFRAHAEAGEGNIRDFVRGAAADAEKSYAA
                     LRRRPPAQNYEIAYASAVFATPQDDGLHLMWFGDCAALLRTPDGTFTFLGDTMTKRES
                     ERARVERLVKPGGRGPAAAGVRDEFLPALRAARNHVNTGEDWLFAPDAACADHANETR
                     ISTPSAARVLLASDGFLALASDYARYTPQTLFAAAETRGLAALGEELRAIEAADPEGV
                     RFPRFKRSDDATALFLVLAA"
     gene            complement(3352..4563)
                     /locus_tag="VGM72_11245"
     CDS             complement(3352..4563)
                     /locus_tag="VGM72_11245"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEY4265883.1"
                     /translation="MSEPATQSVMRALHADGGEARFVGGAVRNALLGEPVGDVDIATS
                     LTPDEVMARLTRAGLGAVPTGIAHGTVTAVCNGKPFEVTTLRRDVETDGRHAVIAFAT
                     DWREDAARRDFTINALYADENGTITDYFGGLQDLAARRVRFVGDPRLRIREDYLRILR
                     LFRFHAWYGTGEIDEAALAASAAGKSGLQRLSGERVQKELLRLLEAKDPMAVLRAMQD
                     SGILAELLPGDSQLQRLARLIAIENANAIAPDAPLRLAALLPDGGVVARVLAGRLKLS
                     NALRGRIVEATEKDDRIAASLDEADARKLLYRLGTERFRDCVLLQWAAAGADAGDVAW
                     RRLLRLAEDWTAPIFTLDGNDVMALGFEEGQEVGVLLREIEQRWVAGDFAAGRPQLLE
                     WLAHAAKKPRG"
     gene            complement(4608..4880)
                     /locus_tag="VGM72_11250"
     CDS             complement(4608..4880)
                     /locus_tag="VGM72_11250"
                     /inference="COORDINATES: protein motif:HMM:NF040327.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6111 family protein"
                     /protein_id="HEY4265884.1"
                     /translation="MIRRIFYEVLLFLLPFALYAAYWRLAKRDETVSAHDHPWTVLFA
                     SGLVLVAASFLIWGITEGAGERGIYIAPHVENGRVIPGYVEPGPAK"
     gene            complement(4877..5494)
                     /locus_tag="VGM72_11255"
     CDS             complement(4877..5494)
                     /locus_tag="VGM72_11255"
                     /inference="COORDINATES: protein motif:HMM:NF007980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEY4265885.1"
                     /translation="MVNGGIGQKTGTELAAGSLSRERIRDSLRRTPPELRPSGPRFRP
                     AAILLPIVERAEPYALFTKRTDDLPSHAGQICFPGGRYHTDDETLIQTALREVEEEIG
                     LSPDVVDVVGFLDSYETLNTGFTILPVVGYLRPDFSLIINPREVAEAFEAPLGYILDP
                     KNHALKSVERDGVLREFYAIEYASHVIWGATAAMIVNLSERLGLR"
     gene            5736..6773
                     /locus_tag="VGM72_11260"
     CDS             5736..6773
                     /locus_tag="VGM72_11260"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY4265886.1"
                     /translation="MSSTVSGADTQFKGFAPGMESADAEALADELGARFSAAREAISS
                     FIFGQKSVVDQVLITLLAGGNGLLIGVPGLAKTRLVDTLGTVLGLDSKRIQFTPDLMP
                     GDIVGSEVLEEDEHGRRTFRFIKGPVFCQLLMADEINRASPRTQSALLQAMQERHVTI
                     AGQRYDLPRPFHVLATQNPLEQEGTYPLPEAQLDRFLLQIDVGYPDREAEQRMLMATT
                     VGDERPPQRVIDAETLMKAQALVRRLPVGEKVVQAILGLVRSGRPDDGASETVRNFVA
                     WGPSPRASQAFMLAIRARALLEGRFAPSIEDVVALAPAILRHRMALNFSARAEGATIT
                     QVIEGLCQAYL"
     gene            6923..7849
                     /locus_tag="VGM72_11265"
     CDS             6923..7849
                     /locus_tag="VGM72_11265"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY4265887.1"
                     /translation="MASTAYQPFDTRQPERWDRPREEAENLGSALPPLLVEAERLTSG
                     IWLGVHGRRKAGIGETFWQFRRYRHEDPSTAIDWRQSAKSQHVYVREREWEAAETVWF
                     WRDASASMRYASLKAVPEKWERATVLSLALASLLVRGGERIGLLGTGAPPSSGRIALR
                     RMAHRLSQPEEEESDLPPELHIKRQCECVWISDFLQPLDSIESAMKGLAYSGAHGYLV
                     QIIDPAEEDFPFTGRARFEARSVRDTQILGRAETVRANYRRRYDAHSEAVGQLARRLG
                     WSFLRHRTDHSPATALIALYGALGGARAQRGF"
     gene            7853..10657
                     /locus_tag="VGM72_11270"
     CDS             7853..10657
                     /locus_tag="VGM72_11270"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEY4265888.1"
                     /translation="MFAGLIAGLTFGAPYLLWALATLPVIYWLLRVTPPAPKRIVFPP
                     LRLLLGLKATEETPSRTPLWLLLMRLLAAAIAIFALAEPLYDPTPIARGNGPLVLVVD
                     NGWPSAAQWQTRQAAMNRALTGATRDRRAVLIVATAERAPVITQFLDPGKALKTVEDM
                     VPRPWLPDRAKTLTQLQQMKFTGTPQIMWFSDGLDHGNARAFANGLAKLGPLGVFQDE
                     ADKMPLAMKPPDNANTGFGITLTRVPADNQREGRVAALDARGQVLETAPYHFGLRAGE
                     AKTTIALPLELRNDTVRIAVMANESAGSVQLVDARFRRRPVGLVSGGAADTAQPLLSD
                     VYYIERALAPYAELHKGTVDQVLNSGIAVIAISDIGRLSQEEHDRISKFVDEGGVLLR
                     FGGPRLAAQSDDLVPVRLRAGERLMGSSMTWASPQHLTPFGDESPFRGLVIPNDVTVS
                     RQVLAEPSVELADHTWARLTDGTPLVTGTAKGRGWIVLFHVAASPGWSSLPISGLYVE
                     MLRRIVELSEGVRGGAAAAKTGTFPPFQTLNGFGHLEKPYPEATPLRASEMETATVGP
                     LHPPGLYGVQGALIALNAFGSHSVLKPLDVGRSIFSYSGSAAQELKWPMLELALILLL
                     IDAMISLALRGYISLTGHRYRKTAVRAATAILLMLAPVALVLAPGPAFAAPARAPATA
                     EDKILESALDTRLAYVVTGDAEVDAMSKAGLFGLGLELRARTAYEPADPVGVDVEKDT
                     LSFYPLLYWPMAPGEKDLSPVAVAKIDQFMRDGGTILFDTRDSPIGGLGTTPSPGETV
                     LRRLLSKLDIPPLEPAPQDHVLTRTFYLLKEFPGRWSGGQVWVEALPPSDPKNPPANP
                     VAARGGDGVSPIIMGGNDWAAAWARDEMGNPIAAVVPGGEAQRETAIRFGINVVIYAM
                     TGNYKTDQVHVPALLERLGK"
     gene            10665..12887
                     /locus_tag="VGM72_11275"
     CDS             10665..12887
                     /locus_tag="VGM72_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265889.1"
                     /translation="MSSFGIAFAPHLPLWLLAVFGGIALAILAFSVYRQAHGAWARLL
                     AFAVILLALANPLIVKETREGLSNIVALVIDRSQSMEIGTRKADSEKALAALREKLKA
                     MNVEVREAEVRTNADAGDAGGTALFSALNGALSDAPPDRVAGAIVITDGEVHDAPDPK
                     TFHPGAPFHVLLTGSPNERDRKLTVVRATRFNIVGKEAEITFRIDDFGGSQGANAQLS
                     MRIDGQDAGKSTVPVGKDTTIKVAVAHGGENVVELEAAPGPAEQTLQNNRAAVIINGV
                     RDRLRVLLVSGEPHAGERVWRNLLKADPSVDLVHFTILRPPDKQDGTPINELALIQFP
                     TRELFAEKLNEFDLVILDRYQNRDILPLAYFDNLARYVENGGALLLSSGPEFADESSI
                     YRTPLAAVLPAQPTGEVVSQPFKPQITKAGFAHPVTRDLPQSNEAPELFSEPVPGIKS
                     EDAAPSSNQTASTAPVPPAPARATAPAATPGPAAGPAAQPGQPDWGRWFRIIAAQKLS
                     GNTIMSGPGDRPLLVLDHVQKGRVAQLLSDQIWLWARGYENGGPQAELLRRLAHWLMK
                     EPELEEERLTAEVTGGELRITRQTMSDKAASVNVTTPTGKTQTVALSPASPGKFAGRV
                     KADELGLYRMNDGTLNAVAAAGPLNPREVADMRATDTILKPYADATGGGVQWLSDGIP
                     DLRAVDQGSTTRGNGWFGIERRGAYRVTSVDSDQLMPPWLALLLVLTAILFAWRRESV
                     "
     gene            complement(12884..14164)
                     /locus_tag="VGM72_11280"
     CDS             complement(12884..14164)
                     /locus_tag="VGM72_11280"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4265890.1"
                     /translation="MNDDAVFAKCAWRLIPFIFVLYLVNFIDRVNVGFAALTMNGDLN
                     FSPAVFGFGGGIFFLGYSLFQIPANLILERVGARRWIFLIILVWGAVSACTAFITNAT
                     GFYAVRILLGIAEAGFFPGMVLYLTYWFPKAYRARLVANFMLAIPFANIVGGPVSGLI
                     LGMDGLGGLHGWQWLFLIEGMPACAIAFAVLAWLPSGPAEARWLNAEEKRVIAARLAS
                     EETVRHRNLLTAFLDPRVWALGLVYLGYAISYYGVQLWLPQIVQAMGFSNLATSFLVA
                     LPFAATMIAMFLWGRSSDSAGERIWHVAIPALVAMASLIAASIAASNLVIFLALTIAV
                     VSLMSLQGPFWALPQTYLSGVAAAGGIALINMLGTGAGGFVGPYVVGVLKNATGGYAV
                     PIAVLAVAPAMTAIVVLSLKSGVAAKPASRLASG"
     gene            complement(14310..>14544)
                     /locus_tag="VGM72_11285"
     CDS             complement(14310..>14544)
                     /locus_tag="VGM72_11285"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY4265891.1"
                     /translation="MPVMAASPLGGVEDAHTLLGDRTLTQIAATHGCSPAAVALAFVM
                     RSGNVIAIPESGTPAHVKENAAALSIRWVSASS"
BASE COUNT         2731 a         4731 c         4521 g         2561 t
ORIGIN      
        1 ccaaggactc ggcaggccgg cttgcctcgg ccagaatagc ggcccggctc gaaagagcct
       61 ccgccgagcc gggctcgatc gaaagcgcgg tatcgataca ggcgagcgcg tcctgccatc
      121 tctgaagacg ccgcagcacc gcgctttgga ggcgccagcc ttcggcgaaa cgcggcctga
      181 gcgcgagcgc ccggcggaac gtaagcaggg cctcgtccaa ttccccattt ttcagccggt
      241 tgcgcgcaag cccatagagc gcctcgaaat ccggcggctg cgcggcccat ttgtcctgat
      301 ggctgtcctg atgacgattg gccgtttcgg aagcgccgcc cacccgattg aggctggtgg
      361 ctgcggcttg cagcgaatct ggcacggaat acgcggacgg cttacgcatg ggacgctatg
      421 gccggagctt caccgcagaa tcaataaccc cggcttttcc ttgcaaattt ttaaaattgt
      481 agggaaatgt actacatttc gtcttcggca cagatggatg caaaaagccc ggtgcgaaaa
      541 aacccagagg gagcttgtaa tcggcgcgac gcacgttcgg acttattctt gccttccgct
      601 tggccgtgcc gcgagactct ggcgaagccg ttccaccatg ccggcaatcg gtgcgctcca
      661 atccttgtcg attttttgcc ggaaaaggcg cgcggacgga taccaggggc tgtcgcttcc
      721 gttgagcatc cagcgccatt ccgggacata tttgagcgcg atccaggtgg gccgccccag
      781 cgcaccggca agatgggcga tggatgtgtc gcaggtgatg atgacatcga aatgttgcat
      841 cgccgcggcc gtatcggcaa aggcatcggg gcccgcatcg aaatccgcgc ccagggtttc
      901 cacctgcatc cctgcgggca gatccgccaa ttgctcggcg ccctcattct tctgaatgcc
      961 gatcaaacgc aggccaggaa tttgtgagag cggataaaat tcgcggagcg gcacgccgcg
     1021 cccgacgatc tccacgccat tgtgccagcg cgcgccctgc caggcgatgc cggcgcgaaa
     1081 cccgtgcgga ccgatggtct tgcgccaatg atcgacgcgt gacggctcga ccgcgagata
     1141 gggaatatcc agcggaatat ccccaaattc caatccgaga ccgcgcggta gactttgcag
     1201 gtcgcattga aaatcgaaac cgcggtccgg caaagccgcg agcaggcgca tatcgcccgg
     1261 cagaccggaa agcagccggt ggagctttgc cggtacgaga aagctcacat ccgcgccgcg
     1321 actcccaagc agcggcacca gccggctgaa ctggatgatg tcgccaaggc cctgctcggc
     1381 gtaaaccagg atggagcagc ccgcgagatc ctcgccggtc caatggggcg cgtccacacc
     1441 gctcccgcgc gcagggtaat tgcgcatccg ccagcggttt tcgtaatcgt cccaggcgct
     1501 cctttccata cggccgagcg ccaatttgca cacgccgcgg ctgaacagga tctcgggatg
     1561 atccggcgcg cgggcgagcg cgcgatcata gcttgccagc gcctcctcgg gccgcttgag
     1621 cgcaagcagc atgacgccgc gattgtaatg ggcttcggcg aaatcgggtt ttatggccaa
     1681 cgcgcggtca tagctttcga gcgcggcttc caggtggccg agtttctgga gcgcatttcc
     1741 ccgattgctc agcgcctcgg caaaatccgg cgcgaatgcg agggcccgat catagctttc
     1801 gacggcctcg ccaaagcgcc ccaaatgctg caaggcaagg ccgcgattgt aatgggcgtc
     1861 cacaaaatcg gacgtccgcg caatcgccgc atcatagctc gccagcgctt cgtcatggcg
     1921 ttcgagtgcc cacaggaccg taccgcgttt gttgaaagcg gccgcataat cgggcttcag
     1981 cgcgatggcg cggtcctgag cggcaagagc ctcctcatac tggccgagac tgtgccgcgc
     2041 gatgccgcga ttgtgatgca gaatcggaga gaggggactg agcgcgatgg ccctgtcata
     2101 gcattcgagc gcatcggcaa accgcccaag ctcctgcagg acattgccgc gatgaccatg
     2161 cgcatcggca tcgtcgggtt tcagcaggat cgcgcgatcc aggctcgcga gggcttcggc
     2221 gttgcggtca agacgcgcca acgccaccgc gcgatcgcga tgggcggatg cggcattggg
     2281 attgagggct agtgcgcctg cgattctttg ctcggcgatt tggaattgcc cgcgctgcag
     2341 cgcgatgatg cccaaaagat aatgcgcgcc gaaatgcgtg tgcccggctt tcagcaagcc
     2401 aacgcaaaca cgctcagcgt catcccaccg tccgtgccga tgaaaagcca cggctttcgc
     2461 gaattcgtct gccggtggcg atggcgccat gatgggttca cgcggctagc acgaggaaaa
     2521 gagcagtggc atcgtcgctg cgcttgaagc ggggaaagcg cacgccttcc ggatcggccg
     2581 cttcgatggc gcgcaattcc tcgccgagcg cggcgagccc gcgcgtctcg gcggcggcga
     2641 acaatgtttg cggcgtatag cgcgcataat cgctggcgag cgccagaaac ccatcgcttg
     2701 cgagcagcac gcgcgcagcc gatggggtcg atatccgagt ctcgttggca tgatcggcgc
     2761 atgccgcatc gggcgcgaac aaccagtcct cgccggtgtt cacatgattg cgcgccgcgc
     2821 gcagtgcggg caggaattca tcgcgcacgc ccgccgccgc cggtccgcgc ccgccgggtt
     2881 tgacgagccg ctccacgcgg gcccgctcgc tctcacgctt ggtcatcgta tcgccgagaa
     2941 acgtaaatgt gccgtccggc gttcgcagca atgccgcgca atcgccgaac cacatcaggt
     3001 gcaacccatc gtcctgcggc gtcgcgaaca cggccgaggc ataggcgatc tcgtaattct
     3061 gggccggggg acggcggcgc aacgccgcgt aacttttctc ggcatcggcg gcggccccgc
     3121 gcacgaagtc gcggatattc ccctcgcccg cttcggcatg ggcgcggaac cgccgcgcgc
     3181 cgaattgcgc gagccattgg gcatcggatt ttcccggcat caattgctcg ccgaggcccg
     3241 tcgcgccgtc gaacacagcc gccgccacgg gcgaaaacgc ataagcgtct tcattcggtt
     3301 tcgtggcatc gccgggaagg gtcagacttt cgatcagttc cagacgcatg gtcagccgcg
     3361 cggtttcttg gccgcgtggg cgagccattc gagcaattgc ggccggccgg ccgcaaaatc
     3421 gccggccacc caacgctgct cgatctcgcg taggagcaca cccacttcct ggccttcttc
     3481 aaagccaagc gccatcacat cattgccgtc cagcgtgaaa atcggtgccg tccaatcctc
     3541 cgccagccgc agcaaacgcc gccaggccac gtcaccggca tcggcgcccg ccgcggccca
     3601 ttgcagcagc acgcaatcgc ggaagcgctc cgtgccaaga cgataaagca gcttgcgtgc
     3661 atcggcttca tcgagcgagg cggcgatccg gtcatctttt tccgtagcct cgacgatccg
     3721 gccgcgcaac gcattggaaa gcttcaagcg gcccgcgagc acgcgcgcca caacaccacc
     3781 atcgggcaac agcgcggcga ggcgcaatgg tgcatcgggt gcaatcgcgt tggcattctc
     3841 gatcgcaatc agccgggcca gacgctgcaa ttgggaatcg ccgggcaata gttccgccag
     3901 aatccccgaa tcctgcatgg cgcgaagcac cgccatcggg tccttcgctt ccagaagccg
     3961 cagcaactct ttttgcacgc gttcgccgga caaccgctgg agcccggact tcccggccgc
     4021 cgacgcggca agtgccgcct catcgatctc gcccgttcca taccaggcgt gaaagcggaa
     4081 cagcctcagg atgcgcaaat aatcctcgcg gatgcgcagc ctcggatcgc ccacgaaacg
     4141 aacccggcgt gccgcgagat cctgcaagcc cccgaaataa tcggtgatcg taccgttctc
     4201 atcggcgtag agcgcattga tggtgaagtc gcgccgcgcg gcgtcctcgc gccagtccgt
     4261 ggcgaatgcg atcaccgcgt gccgtccgtc ggtttcgaca tcgcgcctga gcgtcgtgac
     4321 ttcgaacggc ttgccattgc acaccgcggt cactgtgcca tgcgcgatac cggtcggcac
     4381 cgcacccaat cccgcacgcg tcaaacgggc catgacctca tcgggtgtca gggatgttgc
     4441 gatgtcgaca tcgcccaccg gctctccgag aagcgcattg cgcaccgcgc cccccacaaa
     4501 ccgcgcctcg ccgccgtccg catgaagcgc gcgcatcacg ctctgcgtag cgggctccga
     4561 catccagcga ttgcgattgg ggtcgagcct cttattcgat tgatcattca cttggcggga
     4621 cctggttcga cgtaacctgg aatcacacgg ccattctcga catggggagc gatatagatg
     4681 ccccgttcgc ccgcgccttc ggtgatgccc cagatgagaa agctcgccgc caccagcaca
     4741 agaccggagg cgaaaagaac ggtccatgga tgatcgtgcg cggacaccgt ttcatcgcgc
     4801 ttggcaagac gccaataagc ggcgtagagc gcaaagggca gcaggaacag aaggacttcg
     4861 taaaaaatcc gccggatcat cgtaacccca gcctctccga caggttcacg atcatcgccg
     4921 ccgtggcgcc ccagatgaca tggcttgcat attcgatggc ataaaactca cgcaaaaccc
     4981 cgtcgcgctc caccgatttc aacgcgtggt ttttcgggtc cagaatataa ccgagcgggg
     5041 cttcgaacgc ttcggccact tcgcgcggat tgatgatgag cgaaaaatcg gggcgcaaat
     5101 atcccaccac cggcaaaatc gtgaagccgg tattcagcgt ctcataagaa tccagaaatc
     5161 ccaccacatc tacaacatcg ggggatagac cgatctcctc ctccacttcg cgcagtgccg
     5221 tctggatcag ggtctcatcg tcggtgtgat aacgcccgcc ggggaaacag atttgtccgg
     5281 cgtgggaggg caaatcgtcg gtgcgcttgg tgaacaatgc ataaggctcg gcgcgttcca
     5341 cgatcggcag caatatggca gccgggcgga atctcggacc tgacggacgc aattccggag
     5401 gggtgcggcg caagctgtcg cggattcgtt cgcgcgaaag agacccggcg gccaattcgg
     5461 ttcccgtttt ctggccaatc cctccattca ccatggcggt gcacactcct ttcgggcctc
     5521 acgccttttt gtggcggccg ttcccgcagc cgttctatga tgcttggcgc tctctcttag
     5581 gctgttgcat gagcgccgca agggtgcaac atgggcgggg aaggccgtcc aattcccggg
     5641 ctcccatgcc ctgaattaga gagcgtaagg ggcggctcca gtggtgccag gaagacttgg
     5701 aaaccggaac ggataaacta gcggaaccaa tggacatgag ctcaactgtt agcggcgccg
     5761 atacgcaatt caaaggtttt gcccccggca tggagagtgc ggacgccgaa gccctggccg
     5821 acgagttggg cgcacgtttt tcggccgccc gtgaggcgat ttcgagcttt attttcggtc
     5881 agaaatcggt ggtggatcag gtcctgatca cgctgcttgc cggcggcaat gggttgctca
     5941 tcggcgttcc gggcctggcc aagacgcgtc tggtcgatac gctaggcacc gttctcggcc
     6001 tggatagcaa gcgcatccaa ttcacgccgg acctgatgcc gggcgatatc gtgggttccg
     6061 aggtgctgga agaagacgag cacggccgcc gcacctttcg ctttattaag gggccggtgt
     6121 tctgccagct cctgatggcg gacgaaatca accgcgcatc cccgcgcacg caatcggcgc
     6181 tgctgcaggc gatgcaggaa cggcatgtaa cgatcgcagg ccagcgttac gatctgccgc
     6241 ggccgttcca tgtgctcgcg acacaaaatc cgctggaaca ggaaggcacc tatcctctgc
     6301 ccgaggctca gcttgaccgc tttttgttac agatcgacgt cggctatccc gaccgcgagg
     6361 cggagcagag aatgctgatg gcaacgacgg ttggcgacga acggccaccg caacgggtga
     6421 tcgacgcgga gacgctgatg aaggcacagg ccctggttcg ccgcctgccc gtgggcgaga
     6481 aggtcgtgca ggcgatcctt ggactggtgc ggtccggccg gccggacgac ggcgcgagcg
     6541 aaaccgtgcg gaatttcgtg gcctggggtc cctctccgcg cgccagccag gctttcatgc
     6601 tggcgatccg cgcgcgcgcg ttgctcgaag gacgtttcgc gccttcgatc gaggatgtcg
     6661 tcgcgcttgc gcctgcgatc ctgcgccatc gcatggcgct gaatttctcg gcgcgcgcgg
     6721 aaggcgcgac catcacgcag gtgatcgaag gtctctgcca agcctacctt tgattttccc
     6781 tccccggaaa aggagggata ggatcgcaga gcgatccaag gagggatcga tgtccctgca
     6841 gcatggtact ctgtcccaca tccgatccgc ccgcgcggat cgatctcccg ttttttgggg
     6901 gaggtaacgg atcgcgaagc ctatggcgag cacggcatat cagccttttg atacccgaca
     6961 gccggaacgc tgggaccgtc cgcgcgaaga ggcggagaat cttggttccg cgctgccgcc
     7021 tctgctcgtc gaggccgagc ggctcacctc cggaatctgg cttggcgtgc acgggcgccg
     7081 caaggccggg atcggggaga cgttctggca gttccgccgc taccgccacg aagatccttc
     7141 gaccgcgatc gattggcgcc aatcggccaa atcgcagcat gtctatgttc gcgagcgcga
     7201 atgggaagcg gccgaaaccg tctggttctg gcgcgatgcc tcggccagca tgcgctatgc
     7261 gtcgctgaag gccgtgccgg aaaaatggga acgcgcgacc gtgctgtctc tggcgctggc
     7321 atcgctcttg gtgcgtggcg gggaacgcat cgggcttctg ggcaccggcg caccgccctc
     7381 ttcggggcga atcgcgcttc gccgcatggc ccaccggctc tcacagcccg aggaggaaga
     7441 atccgacctg ccgccggagc tgcacatcaa gcgccaatgc gagtgcgtat ggatttccga
     7501 ttttctacag cctctcgatt ccatcgaatc cgcgatgaag ggtctcgcct acagcggtgc
     7561 gcatggctat ctggttcaga tcatcgatcc ggccgaggag gattttccct tcaccgggcg
     7621 cgcccgcttc gaagcgcgca gtgtccgcga tacccagatt ctcggccgcg cggaaaccgt
     7681 cagagccaat taccgccggc gctatgacgc ccattccgaa gcggtgggac aattggcccg
     7741 caggctcggc tggagtttcc tgcgccaccg caccgaccat tcgcccgcga ccgcattgat
     7801 cgcgctttac ggcgcccttg gcggcgcccg cgcgcaacgg gggttctgaa ccatgtttgc
     7861 tggtctcatt gccggcctga cattcggagc accctatctg ctgtgggcgc ttgcgacgct
     7921 gccggtcatc tattggctgc tgcgggtaac cccgccggcc cccaagcgca ttgtgtttcc
     7981 gccgctgcgc ctgttgctcg ggctgaaagc gaccgaagag acgccgtcgc gcaccccgct
     8041 ttggctgctc ttgatgcggc ttctggctgc ggccatcgcc attttcgcgc tcgccgagcc
     8101 gctttacgat ccgacaccga ttgcacgcgg gaacgggccg ctggtcctgg tcgtcgacaa
     8161 tggctggcct tcggccgcgc aatggcagac gcgccaagcc gcgatgaatc gggcactgac
     8221 cggggccacg cgcgatcggc gggccgtcct gatcgtagca acagccgaga gggctccggt
     8281 catcacgcaa ttcctcgatc ccggcaaggc gctcaagacc gtcgaggata tggtgccccg
     8341 cccgtggctg ccggaccgcg ccaagaccct cacgcaattg cagcagatga aattcaccgg
     8401 aacaccgcag atcatgtggt tttccgacgg gctggatcac ggcaatgccc gcgccttcgc
     8461 caatggcttg gccaagctcg gcccgctggg cgtgttccag gacgaagccg acaagatgcc
     8521 gctggcgatg aagccgccgg acaacgccaa taccggcttc gggatcacgc tgacgcgggt
     8581 tcctgccgac aatcagcgcg aggggcgcgt cgccgctctc gatgcccgcg gacaggtgct
     8641 tgagaccgcg ccctaccatt tcggattgcg cgcgggcgag gccaaaacga ccatcgcgct
     8701 gccgcttgaa ctgcgcaacg atacggtgcg gatcgcggtc atggccaatg aatcggccgg
     8761 ctccgtgcaa ctcgtggatg cacgctttcg ccgccgccct gtgggcctcg tctccggcgg
     8821 agccgccgat acggcacaac ccctgctctc cgacgtctat tacatcgagc gcgcgctcgc
     8881 tccttatgcc gaactccaca agggcaccgt ggatcaggtg ctgaatagcg gcatcgcggt
     8941 gattgcgatt tccgatatag ggcggctctc ccaagaggag catgatcgca tctcgaaatt
     9001 cgtcgatgag ggcggcgtgc tcttacgctt tgggggaccc cgccttgccg cccagagcga
     9061 cgatctcgtg cccgtgcggc tgcgcgccgg cgaacggctc atgggcagct ccatgacgtg
     9121 ggcttcgccg cagcatctga caccttttgg tgacgagagt ccttttcgcg gcctcgtcat
     9181 ccccaatgac gtaactgtgt cccggcaggt gctcgcggag ccttcggtgg agcttgccga
     9241 tcacacctgg gcgcgtttga ccgacggcac gcctcttgtc acgggcaccg ccaaggggcg
     9301 cggctggatc gtgctctttc atgtcgcggc gagcccaggt tggtcctcgc tgccgatttc
     9361 gggcctttat gtggagatgc tgcgccggat cgtagaactt tccgaaggcg tgcgcggagg
     9421 tgccgcggcg gccaagactg ggaccttccc accgtttcag acgctcaacg gtttcggcca
     9481 tctggaaaag ccctatccgg aagcgacgcc tctgcgcgcc tcggaaatgg agacggcgac
     9541 ggtaggaccc ttgcatcctc cgggccttta cggcgttcaa ggcgctctga tcgcgctgaa
     9601 tgcgttcgga tcgcactcgg tcctcaagcc gttggatgtc ggacgttcga tcttctccta
     9661 ttccgggagc gccgcgcagg agctgaagtg gcccatgctt gagcttgcgc tcattcttct
     9721 gctcatcgac gcgatgatct cgctggcgtt gcgcggctat atcagtttga cggggcaccg
     9781 ttaccgcaag accgcggtta gggccgcaac cgccattctt ctcatgctgg ctccggtcgc
     9841 gctcgtgctg gcccccggcc ctgcgttcgc cgcacccgcg cgcgcgcccg ccacggccga
     9901 ggacaagatt ctggaatccg cgctcgatac gcggcttgcc tatgtggtca cgggcgatgc
     9961 cgaagtcgat gcgatgagca aggccggctt gttcgggctc ggtctggaac tgcgcgcccg
    10021 cacggcctac gaaccggcgg acccggtcgg cgtggacgtc gagaaggaca cgctttcctt
    10081 ctatcccctg ctctactggc ccatggcgcc cggggaaaaa gatctttcgc cggtggcggt
    10141 tgccaagatc gaccaattca tgcgcgatgg cggcaccatt cttttcgaca cacgcgattc
    10201 gccgattggg ggcctcggca ccacgccgtc gcccggtgag accgtgctgc ggcggctgct
    10261 gtccaagctc gacatcccgc ctctggaacc tgcgccgcag gatcatgtgc tgacgcgcac
    10321 cttttacctt ctgaaggaat tcccgggccg ctggagcggt ggccaagtct gggtcgaagc
    10381 gctgcctccg tcagacccta agaatccacc ggccaacccg gtcgcggccc gcggcggcga
    10441 cggcgtttcg ccgatcatca tgggcggcaa tgattgggcc gccgcgtggg cccgtgacga
    10501 aatgggcaac ccgatcgcgg ccgtggtgcc ggggggcgag gcacaacgcg agaccgcgat
    10561 tcgcttcggg atcaacgtcg tgatctatgc tatgaccggc aactacaaga ccgatcaggt
    10621 ccacgtcccc gctcttctgg aacggctggg gaaataggag cgctatgtcg tctttcggca
    10681 ttgcttttgc gccacatctg cctctttggc tgctggcggt gtttggcggg atcgcattgg
    10741 cgattctggc cttctccgtc tatcgccagg cgcatggcgc ctgggcgcgg ttgcttgcgt
    10801 tcgccgtcat cctcttggcg ctcgccaatc ccctgatcgt gaaggagacc cgggagggac
    10861 tttccaacat cgtggcgttg gtcatcgacc gctcgcagag catggagatc ggcacccgca
    10921 aagccgattc cgaaaaagca ctcgccgcat tgcgcgagaa gctgaaggcg atgaacgtcg
    10981 aagtgcgcga agccgaggtg cgtacgaatg cggatgcggg cgatgccggt ggcacggccc
    11041 tgttctcggc gctgaacgga gccttgtccg atgcgcctcc ggaccgggtg gccggcgcca
    11101 tcgtcattac cgacggtgag gtgcacgatg cgcccgatcc caagacgttc catcccggcg
    11161 cacccttcca cgtcttgctg accggctcgc ccaatgagcg cgaccgcaag ctgacggtgg
    11221 ttcgcgcgac gcgcttcaat atcgtcggca aggaagccga aatcaccttc cgcatcgacg
    11281 atttcggcgg ttcgcaaggc gccaacgccc agctttccat gcgcatcgac ggccaggatg
    11341 ccggcaagag cactgttccc gtcggcaagg acaccacgat caaggtggcg gtcgcccatg
    11401 gcggcgaaaa cgtcgtcgaa ctcgaagccg cccccggccc ggcggagcag acgctgcaga
    11461 acaatcgcgc ggcggtcatc atcaacggcg tgcgcgaccg attgcgggtg ctgctggttt
    11521 ccggtgagcc ccatgccggc gagcgggtct ggcgcaatct cttgaaggcc gatccctcgg
    11581 tcgacctggt gcatttcacc atcctgcggc cgccggataa gcaggacggg acgccgatca
    11641 acgagcttgc gctgatccaa ttccccacca gggaattgtt cgccgagaag ctcaacgagt
    11701 tcgatctggt gatcctcgac cgctaccaga atcgcgacat ccttccgctc gcctatttcg
    11761 acaatctcgc acgctatgtc gaaaacggcg gcgccctgct gttgtcgtcg ggaccggaat
    11821 tcgctgacga gtccagcatc taccgcaccc cgctggctgc cgtgcttccc gcgcaaccga
    11881 ccggagaggt cgtaagccaa ccctttaagc cacaaatcac caaagcaggc tttgcccatc
    11941 cggtgacgcg cgatctgcct cagagcaatg aagcacctga actgttttca gagccggtcc
    12001 ccggcatcaa gagcgaggac gcagcgcctt cctccaatca gacggcctct acggctccgg
    12061 taccgccggc gcccgcgcgc gcgacagcgc ctgccgcgac accaggaccc gccgcaggcc
    12121 ccgccgcaca acccggtcag cccgattggg gccgttggtt ccggatcatt gccgcgcaga
    12181 agctctccgg caacacgatc atgtcgggcc ccggggatcg gccgctgctt gtgctcgacc
    12241 atgtgcaaaa gggccgcgtg gcacagcttc tgtccgacca gatctggctc tgggcgcgcg
    12301 gctatgagaa tggtggcccc caagccgaac tcctgcgccg gcttgcccat tggctgatga
    12361 aggagcccga actcgaagag gagcgcctga ccgcggaagt cacgggcggg gaattgcgta
    12421 tcacccgtca gaccatgagt gacaaggcgg cgagcgtgaa tgtcacgacg ccaacgggaa
    12481 agacccaaac cgtggcgctc tcgccggcca gtccgggtaa gttcgccggc cgtgtgaagg
    12541 ccgacgagct ggggctctac cgcatgaacg acggcacgct caacgcggtc gcggcggctg
    12601 gtcccttgaa cccgcgcgag gtcgccgaca tgcgcgccac cgacacgatc ctcaaaccct
    12661 acgccgatgc gaccggcggc ggcgtgcaat ggctctccga tggcattccc gatctgcgcg
    12721 ccgtggatca gggttcgacc acgcgcggga acggatggtt cggcatcgaa cgtcgcggcg
    12781 catatcgcgt gacctcggtg gacagcgatc agctcatgcc gccctggctt gcgctcctcc
    12841 tggtgctgac ggcgatcctt ttcgcctggc ggcgcgagtc ggtctaaccg ctcgcgagtc
    12901 tgctcgcggg tttcgccgcc acaccgcttt tcagtgagag aaccacgatg gcggtcatcg
    12961 ccggtgccac agccagcacc gcgatgggga cagcgtagcc gccggtggcg ttcttgagca
    13021 cgcccacgac atagggtccg acgaaaccgc ccgcacccgt gccaagcatg ttgatgaggg
    13081 cgatgccgcc cgcggccgcc acaccgctga gataggtttg cggcaacgcc cagaacggac
    13141 cttgcagcga catcaggctc accaccgcga tcgtcaaggc gaggaagatc acgaggttgg
    13201 acgcggcgat gctcgcggca atgaggctcg ccatcgcgac cagcgccgga atcgccacat
    13261 gccagatgcg ttcgccggcg gaatcgctgg agcgccccca gagaaacatc gcgatcatgg
    13321 tcgcggcaaa cggcaaggcc accagaaagc tggtggcaag attagaaaag cccatggcct
    13381 gcacgatctg cggcagccac aactgcacgc cataatagct gatcgcgtaa cccagataga
    13441 ccaggccgag cgcccagacc ctgggatcga ggaacgccgt caacagattg cgatgccgca
    13501 cggtttcctc gctggcaagc cgtgcggcga tgacgcgttt ttcttccgcg ttcagccagc
    13561 gcgcttcggc cggcccgctc ggcagccagg ccaggaccgc gaaggcaatc gcgcaagccg
    13621 gcattccttc gatcaggaac agccattgcc agccatgcag gccgccgagt ccgtccatgc
    13681 cgaggatgag cccggagacc ggaccgccga cgatattggc gaagggaatc gcgagcatga
    13741 agttcgccac cagccgggcc cgataggcct tcgggaacca ataggtcaga tagagcacca
    13801 tgcccggaaa gaaacccgcc tccgcgatcc cgagcaggat tcgcaccgcg taaaatcccg
    13861 tggcattggt gatgaaggcg gtgcacgccg acaccgcgcc ccacaccaga atgatcagga
    13921 aaatccagcg ccgcgcgccc acacgctcga ggatgagatt ggccggaatc tggaacagcg
    13981 aatagccgag gaaaaagatg ccgccgccga agccgaacac cgccggcgag aagttcagat
    14041 cgccgttcat ggtgagggcc gcgaagccga cattcacccg gtcgatgaaa ttcaccagat
    14101 agagcacgaa gatgaacggg atcagccgcc acgcgcattt ggcgaagacc gcatcgtcgt
    14161 tcatcgaaaa gcccccgcca ttcgcgcagc ccacgtcctg ggaagatacc acagacacgg
    14221 ggccacggat ttagcatatc cataaacccg atgccggctc acccttcgac aagttcaggt
    14281 gaggaaaaag aatcctcatg ctcctgagct taagaggaag ctgagaccca ccgaatggaa
    14341 agcgcggcag cgttttcctt gacatgggct ggtgtgccgg attcgggaat tgcgataacg
    14401 ttcccgctgc gcatgacaaa ggccagcgca accgcggctg gcgagcagcc atgcgtagcg
    14461 gcgatctgcg tcagcgtgcg gtcacccagc agggtgtggg cgtcttcgac gccgccgagc
    14521 ggagaagccg ccatcaccgg cata
//
LOCUS       DASYEG010000262         8709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2850313, whole genome shotgun sequence.
ACCESSION   DASYEG010000262 DASYEG010000000
VERSION     DASYEG010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 8709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8709
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2850313"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VGM72_11290"
     CDS             <1..583
                     /locus_tag="VGM72_11290"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4265892.1"
                     /translation="FNFFSHGTQDLYPTFLQQRFSPATVSIIAVIYNIGAILGSLSFG
                     MLSERIGRRRAIVLASLISIPVIPLWVFAGSPMWLAVGSFLMQFMVQSAWGIVPVHLN
                     ELSPPEVRGTFPGFVYQLGNLLASVNATIQSGYADAHNHDYSVILAIVAACAAVAIAL
                     LVGFGIERKGVHLGTGEEESPQPLMKLTPQEQE"
     gene            complement(561..2063)
                     /locus_tag="VGM72_11295"
     CDS             complement(561..2063)
                     /locus_tag="VGM72_11295"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEY4265893.1"
                     /translation="MARVLWRATVIVHRYLGVAVGLLMLVWFASGIVMMYVPYPGLTG
                     KDRLVPLGPIAWAACCSLDAQNIAGDEPIRAVQIESMAGEPVLFVRPEGRPPRLSSLA
                     SSGPSLDIDDAKARAVVAQAAPRIIGQAAEPVAMELIERDQWTVGDGGDGNRPLYRFT
                     FADPAGTQIYVSSTTGEIVLWTHAAQRFWNWFGAVPHWLYFTELRKNGPLWAQIVIWT
                     SILGGFLTVLGLYLGIAQFKRGRSGRLSPYRGWFYWHHIVGLVFGIVTLTWVVSGTFS
                     MNPWGFLEGRGGDERTRLAGELPSWGVIRASIEALKANPPPDAVRIASAPFGGRLFWL
                     ASRRDGSVTRLDAQGRPAPVEMSDLHAAAQRLAQGSSIESQGAIAEEEAYYFNFSIGE
                     REDRAPLPAYRVVLNDSDHTRYYLNPETAQLVRKVDANSRGQRWLFSGLHRLDFAQWL
                     RLRPVWDVVMLFLLFGGLAGTGTGVYLALQRIKRDLTFRRQPKPIPVPEA"
     gene            complement(2147..4165)
                     /locus_tag="VGM72_11300"
     CDS             complement(2147..4165)
                     /locus_tag="VGM72_11300"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4265894.1"
                     /translation="MRSKIRLARALALASTSYLTLSGAAYAQTLAAAGVPEQVMITGR
                     LEEDLPARLAELGTRVNTVSAGEIQNGGYLDVAQSLQTLVPGLFIAPKNGPFDYVDVS
                     FQGSRTQDVLWTVDGVRINNRLYGGTTPLDTLPATMIERIEVLEGPQALFYGTQGIAG
                     AINIVTKDFSDRPDGRLNIGGDTNNGRHVDGYFRDTLSGGHHIVVYGSEDKSEGFQPF
                     RDQDYQPSQTDRRRAYDVLTFGGKYAYDFSPDMRFTLSEQHTDAKLDDASTQGQLQAF
                     NERNEDVLSAKFDYTMSDKLQFFLKGYYHWWYAHYTQFDNDIDPVTGALTGGVTVVDN
                     HDFWGFTDYGANALVKFTPTPELDTYLGYDYQNYSGNDVVLVIGKQTEHVHAIFGEVA
                     STPVFLSNVRLAAGFRYNDPSVGRSATVWNVTGRWDVTPELFVKGTVGTAFRLPTAEE
                     LFANDPNDERGNPDTKPEQSTNVNASVGGNFGMNMFHWEIVGFARDVKNLIGIQDFDA
                     ATNQDVFGNTPGTVRVRGGELILDAALTSEFSGTFSYTHSDAKDSTHLQIRRVPEDLV
                     KGTLDYHPMNLPFGLFASVNYFGDTFETLGGIREKYGDVAVFDIGGRVFLDMDRHHRL
                     DLNLQNAFDKKYATHLTRGFEDVGGASYLVSDLGLPRTLSLRYTYNFF"
     gene            complement(4336..4812)
                     /locus_tag="VGM72_11305"
     CDS             complement(4336..4812)
                     /locus_tag="VGM72_11305"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY4265895.1"
                     /translation="MSQTHNLRFTLNGRPVTAAVAPHRNLVELLHELGLFGARESCGQ
                     GLCGCCTVLVDDVAVSGCLYLAVMADGKNITTVEHLDASGVLSPVQQAFIETGAFQCG
                     FCTPGFVLMTHQLLARNPDPSEDEIRHYLSGNLCRCAAYPEIIEAVRLAAKLQKRP"
     gene            complement(4809..7079)
                     /locus_tag="VGM72_11310"
     CDS             complement(4809..7079)
                     /locus_tag="VGM72_11310"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY4265896.1"
                     /translation="MNQMGRSMARLEARSKVTGRADYVHNMRVPGMLHAKIFRSTVAH
                     GRIISIDTVAAAEMDGVYAVITGEDIKKIIPQPYFGPAFHDQPILALEKVHFIGEPVA
                     VVLSADPHIAAQAVQMIAAEYEELEPVFDEVEATTSAAIVHEELKPAGTFADLKHLAG
                     RKHTNITLDYQLRRGDVEAGFAASDHVFEHTFRTGQVLHVPLESHASLAIPGNDSLII
                     HTGTQTPSFVRSEVARLLGWPENRVSVRVPFLGGGFGAKVYVKMEALAAALALIVRHP
                     VKIALTMEEQFYTISKHAVTFRIKSGVTKDGRVIARACDVWWNGGAYADIGPRVAQKT
                     GFTAAGPYDIEHVRIDSTEVYTNRPPAGALRGFGVPQLVWAYESHTDMIARELGLDTI
                     EFRRKNILRDGRPHATGTIMKDAAIDLVLDRLAEEMNWSADFDRGTGTVKRGRGIAIG
                     LKASISPTTSGAIVNVSADGSVVLFTNTIDMGQGSDTAMAQLVAEVLNMTAESVKVVH
                     GDTDAAPYDMGTLGSRSTFHMGNAVKLAAEDARAKLTALAAEVGLPSGTNYDVKEIFR
                     KRYGMQAGNVIGTGTYVPRYTSPDPKTGQSDNVTPFWMVGGTGVEVEVDTETGHVRVT
                     KMINVADAGRPINPKICRTQISGGAIMQLGFTLYEDMVFDGGQVTNPSFADYKIPGFR
                     DVPPMESGVVAAVQENGPFGAKGIGESATFGVSPAIANAIHDAVGIRLTELPIKPEAL
                     LRALRAVEGRPLAVDE"
     gene            complement(7076..7933)
                     /locus_tag="VGM72_11315"
     CDS             complement(7076..7933)
                     /locus_tag="VGM72_11315"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEY4265897.1"
                     /translation="MIPFDWVEPESLKEAVSLLDPEDPAVRPIAGGTALMLMMKTGFF
                     VPSRLVSLARIDSDYAMLRLNGDGSLRIGSMVSLAQLENSHEAAAHFPVIKGALRTLS
                     NIRVRNVARVGGALGHGDPHMDLPPLLAMLGGRVSLIGPEATREIAVEDLYLGYYQTV
                     IKNNELIAEAILPPLNGARCAYVKVTSRSADDWPALNIGVKLEMADAGTLARARIVIG
                     AAAERLTRLAATEKLLSNAVSDKTTIARAGECAAAEARFVSDAHGSASYKKELLRVYL
                     ARAIREALA"
     gene            complement(8004..>8709)
                     /locus_tag="VGM72_11320"
     CDS             complement(8004..>8709)
                     /locus_tag="VGM72_11320"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF044283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiD family decarboxylase domain-containing
                     protein"
                     /protein_id="HEY4265898.1"
                     /translation="MPEGPFAEFTGYIAGERLPRPTIRVTAITHRNDPILRGTVEGAL
                     PKSYSENAICSSIMRSAIAWNVLDRAGIPGVTDVWGPPVHGTMNLNVQIKQTYRNQAK
                     QVANAIWGSSASHVRYKHVTVVDDDIDIHDYAAVDWAIAYRVNAGENDIVIMPSTFGA
                     GLDPSTRKRDRNIALFGSGKWNRVLIDATINLDYDPDPDFGGMRFPPTVWPAEADVDA
                     VTARWAELGLGKRKTD"
BASE COUNT         1662 a         2713 c         2672 g         1662 t
ORIGIN      
        1 tttcaatttc ttcagccacg gcacgcagga cctctacccg acgttcctgc agcagcggtt
       61 ttccccggcc accgtcagca tcattgcggt gatctacaat atcggcgcga tcctcggcag
      121 cctgagcttc ggcatgctgt cagaacggat cggccggcgg cgtgccatcg tgctggcctc
      181 gctgatctcc attcccgtca tcccgctatg ggtgttcgcc ggcagtccga tgtggctggc
      241 cgtgggttcc ttcctgatgc aattcatggt gcagagcgcc tggggcatcg tgccggtcca
      301 tctgaacgag ctctcgcccc ctgaggtgcg cggcacgttt cccggtttcg tctaccagct
      361 tggcaatttg ctcgcgtccg tcaacgcgac gattcagtcg ggctatgcgg atgcgcataa
      421 tcacgattac tcggtgatcc tggcgatcgt ggcggcgtgc gcggcggtcg ccatcgcgct
      481 gctggtgggc tttgggatcg aacgcaaggg cgtccatctc ggcaccgggg aggaggaatc
      541 tccgcaaccc ctgatgaagc tcacgcctca ggaacaggaa taggtttggg ctgccgccgg
      601 aaagtaagat cgcgtttgat ccgctggagc gcgagataaa cgccggttcc ggtgcccgcg
      661 agcccgccga acagcagaaa cagcatcacc acgtcccaaa ccgggcgcag cctgagccat
      721 tgggcgaaat ccagacgatg caacccgctg aacagccaac gctggccgcg gctgttggca
      781 tcgaccttgc gcaccaattg tgcggtctcg ggattcagat aatagcgcgt gtgatcggaa
      841 tcgttcagca caacgcgata ggcaggaagc ggcgcgcgat cttcgcgttc gccaatcgag
      901 aagttgaaat aatacgcttc ctcctctgcg atggcgcctt gcgattcgat gctgctgccc
      961 tgggccaggc gttgcgcggc ggcgtgaaga tcggacatct cgaccggcgc aggacggccc
     1021 tgcgcgtcga gccgcgtgac ggaaccatcg cgcctggagg ccagccagaa aagcctaccg
     1081 ccaaacggcg cgctcgcaat gcgcaccgcg tcgggcggcg ggttcgcctt caacgcttcg
     1141 atggaggcgc ggattacgcc ccacgacggc agttcaccgg cgaggcgcgt gcgttcatcg
     1201 ccgccccttc cttccaggaa gccccatgga ttcatcgaga acgtgccgct caccacccag
     1261 gtcagcgtca cgatgccgaa caccagcccg acgatatggt gccaatagaa ccagccgcga
     1321 tagggcgaca atcgcccgct cctgccgcgc ttgaattgcg cgatgccgag ataaagcccg
     1381 agcacagtga ggaacccgcc gagaatcgag gtccagatca cgatctgcgc ccaaagcggt
     1441 ccgttcttgc gcagctccgt gaaatagagc cagtgcggca cggcgccgaa ccaattccag
     1501 aaacgctggg ccgcatgggt ccacagcaca atttctccgg tggtgctcga cacataaatc
     1561 tgggtgccgg cgggatcggc gaaggtgaaa cgatagagcg ggcggttgcc atctccgccg
     1621 tccccgacgg tccattgatc gcgctcgatc aattccatcg ccaccggttc cgccgcctga
     1681 ccgatgatcc ggggggctgc ttgcgccacc acggcccgcg ctttggcgtc atcgatatcg
     1741 agcgacggtc ccgaagaagc aaggctcgac aggcgcggcg gccggccttc gggcctgacg
     1801 aaaagcacgg gctcgcccgc catgctctca atctgcacgg cgcgaatggg ctcatcgccg
     1861 gcaatgttct gagcgtccag cgaacagcag gccgcccaag cgatcggccc gagcgggacc
     1921 aggcgatctt tcccggtcag acccggatag ggcacgtaca tcatgacgat gcccgacgcg
     1981 aaccagacca gcatcaagag gccaaccgcc acgccgagat agcgatgaac gataaccgtt
     2041 gcgcgccaga gaacccgtgc catgtccagc ctttatgcgg gccgcgtcag tataggagag
     2101 tcaacgcctt cgggcgagcg gcatgggccg cccgccccca cgcacatcag aagaaattat
     2161 aggtgtaacg cagcgacaag gtccgcggca ggcccagatc ggacacgaga taggacgcgc
     2221 caccgacatc ttcgaacccg cgcgtcagat gcgtcgcata tttcttatcg aaggcattct
     2281 gcaaattcag atcgaggcga tggtggcgat ccatgtccag aaatacccgc ccgccgatgt
     2341 cgaacaccgc gacgtcgcca tatttctcac ggatgccgcc gagagtttcg aacgtgtcgc
     2401 caaaataatt gaccgacgcg aagaggccga acggcaggtt catgggatga taatcgagtg
     2461 ttcctttgac cagatcttcc ggaacgcgcc ggatttgcag atgggttgaa tccttcgcgt
     2521 cgctgtgggt gtagctgaag gtgccggaaa attcggacgt tagagccgcg tcgagaataa
     2581 gttcgccgcc gcgcacgcgc accgtgccag gggtgttgcc gaagacatcc tgattggtcg
     2641 ccgcatcgaa gtcctgaatg ccgatgagat ttttgacatc gcgggcaaaa cccacgatct
     2701 cccagtgaaa catgttcatg ccgaaattgc cgccaaccga tgcgttcaca ttggtgcttt
     2761 gttcgggctt ggtatccgga ttgccgcgct cgtcattggg atcgttggcg aacaattcct
     2821 cggcggtggg caggcgaaag gccgttccca ccgttccctt gacgaagagt tcgggcgtga
     2881 catcccaacg gccggtgacg ttccacaccg tcgcgctacg ccccacactc ggatcgttgt
     2941 agcggaagcc cgcggccagc cggacattgg aaaggaaaac cggcgtcgac gcgacttcgc
     3001 cgaagatggc atgaacatgt tccgtctgtt tcccgatcac caggacgacg tcgtttccgg
     3061 aatagttctg gtagtcgtaa cccagatagg tgtcgagttc tggtgtcggc gtgaacttca
     3121 cgagcgcgtt cgcgccgtaa tcggtgaagc cccaaaaatc atgattgtcc acgaccgtga
     3181 caccgccggt gagcgcgccg gtcacgggat cgatgtcgtt gtcgaactgg gtgtaatgcg
     3241 cgtaccacca atggtaatag cccttgagga agaactgcag cttatcgctc atcgtataat
     3301 cgaacttggc gctcagcacg tcttcattgc gttcgttgaa ggcttgcagt tggccttgcg
     3361 tggaggcgtc gtcgagcttc gcgtcggtgt gctgctccga aagggtaaaa cgcatgtcgg
     3421 gcgagaagtc ataggcatat ttgcctccga aggtcagcac gtcataggcg cgtctgcggt
     3481 cggtctggct cggctgatag tcttgatccc ggaacggctg gaagccttcg gatttgtcct
     3541 ccgagccgta gaccacgatg tgatggccgc ccgacagcgt gtcgcgaaaa tagccgtcta
     3601 cgtgacggcc attgttggtg tcgccgccga tattcaggcg gccgtccggt ctatcggaga
     3661 aatccttggt cacaatattg atggcgcctg cgattccttg cgtgccgtag aacagcgcct
     3721 gcgggccttc cagcacctcg atgcgctcga tcatggtggc gggcagcgtg tcgagcggcg
     3781 tggtgccacc atagagccgg ttgttgatgc gcaccccgtc taccgtccac agcacatcct
     3841 gggtgcgcga accctggaac gagacgtcga cataatcgaa cggtccgttc ttgggggcga
     3901 tgaaaagacc cggaaccaac gtctggagcg actgcgccac gtcgagataa ccgccgttct
     3961 ggatttcgcc cgcactgacc gtattgacgc gcgtccccaa ttcggcaaga cgcgcgggca
     4021 aatcctcttc caggcggccg gtaatcatca cctgttccgg aacgccggcg gccgcgagtg
     4081 tttgcgcgta tgccgcgccg gacaacgtca agtaacttgt gctcgcgagc gccaaggcgc
     4141 gcgcaaggcg gatttttgaa cgcaagaact cctcccccag ctatgaccat gcgtccgaga
     4201 acacggctcg cacgtgtctt ccgtgctcaa tacggggaag aaggggcgtt ccctcacgcg
     4261 catcacattt tttgtcgcgt gtggttttgt tgaatttttt gctgagggaa accgcgcccg
     4321 tctgcgtaaa ggcgcttaag ggcgcttctg caattttgcg gcgaggcgaa ccgcctcgat
     4381 gatttccgga taggccgcgc agcggcagag attacccgac agatagtgcc ggatctcatc
     4441 ctcgctggga tcgggattgc gcgccaggag ctgatgggtc atcagcacga agcccggggt
     4501 gcaaaacccg cactggaatg cgccggtctc gatgaaggcc tgctgcaccg gcgaaagaac
     4561 accgctcgca tccagatgct ccactgtggt gatgtttttg ccgtcggcca tgaccgccag
     4621 atagagacag cccgacaccg caacatcatc gaccagcacc gtgcagcagc cgcagaggcc
     4681 ctggccacag ctctcgcgcg cgccgaacag cccaagctcg tgcaacagct cgaccagatt
     4741 gcgatggggt gccacggcgg cggtgacggg ccgcccgttc agcgtgaagc gcagattatg
     4801 ggtttggctc attcgtccac cgccaggggg cggccttcga ccgcgcggag cgcgcgcaac
     4861 aacgcctccg gcttgatcgg caactcggtc aggcggatgc caaccgcgtc atggatggca
     4921 ttggcgatgg ccggcgacac gccgaaggtg gccgattcgc cgataccctt cgcaccgaag
     4981 gggccgtttt cctggaccgc ggctaccaca ccggattcca tcggcggcac gtcgcgaaag
     5041 cccgggattt tataatcggc aaaggatgga ttggtcacct gcccgccgtc gaacaccatg
     5101 tcctcataca gggtgaagcc caattgcatg atggccccgc ccgaaatctg cgtgcggcag
     5161 atcttcgggt tgatgggcct gccggcatcc gcgacgttga tcatcttggt cacgcgcaca
     5221 tggccggttt cggtatcgac ctcgacctcc acgcctgtgc cgccgaccat ccagaacggc
     5281 gtcacattgt cggattggcc ggtcttcgga tcaggcgagg tgtaacgcgg cacataggtt
     5341 ccggtgccga tcacattgcc ggcctgcatg ccgtagcgtt tgcggaaaat ctccttcaca
     5401 tcgtaattgg tgccggaggg aagaccgact tcagccgcca gtgcggtgag tttcgcgcgc
     5461 gcgtcttcgg cggcgagctt gaccgcattg cccatgtgga aggtggagcg cgacccgagc
     5521 gtgcccatgt catagggcgc ggcatcggta tcgccatgca cgaccttgac gctttcggcc
     5581 gtcatgttca gcacctcggc cacgagctgc gccatggccg tgtccgagcc ctgccccatg
     5641 tcgatggtat tggtgaagag caccacgctg ccatcggcgc tgacattgac gatagccccg
     5701 gaggtggtgg gcgagatgga cgccttcaac ccgatggcga taccgcggcc gcgtttgacc
     5761 gtgcccgtgc cgcggtcaaa atccgcgctc caattcatct cctcggcgag ccggtcgagc
     5821 accagatcga tggccgcgtc cttcatgatc gtgccggtgg cgtgcgggcg gccgtcgcgc
     5881 aagatgttct tgcggcgaaa ttcgattgtg tcgagcccaa gctcgcgcgc gatcatgtcg
     5941 gtgtggctct cataggccca caccaattgc ggcacgccga aaccgcgcag cgcgccggcc
     6001 ggcggtcggt tggtgtagac ctcggtggaa tcgatccgca catgttcgat gtcgtaggga
     6061 cctgccgccg tgaagccggt tttctgcgcc acacgcggcc cgatatcggc ataggccccg
     6121 ccgttccacc acacatcgca ggcacgcgcg atcacgcgcc cgtccttggt gacgccgctt
     6181 ttgatgcgga aggtcacggc gtgcttgctg atggtgtaga attgttcttc catggtgagc
     6241 gcgatcttca ccgggtggcg cacgatgagc gcgagtgccg cggccagcgc ctccatcttc
     6301 acatagacct tggcgccaaa gccgccgccc aggaacggca cccgcacgct cacgcggttt
     6361 tccggccagc ccagcagccg cgcgacctca gagcggacga atgagggggt ctgcgttccg
     6421 gtgtggatga tgagcgaatc atttcccggt atggccagcg aagcgtgcga ttccagcggc
     6481 acatgcagaa cctgcccggt gcggaatgtg tgctcgaaca catgatcgga cgcggcaaag
     6541 cctgcttcca catcgccgcg ccgcaattga taatcgagcg tgatgttggt gtgcttgcgg
     6601 ccggcaaggt gtttgagatc ggcgaaggtt cctgccggct tcagctcctc atgcacgatg
     6661 gccgccgacg tcgtggcctc gacctcatcg aacaccggct ccaattcctc atattcggcg
     6721 gcgatcatct gaaccgcctg tgcggcgata tggggatcgg cagacaggac caccgccacc
     6781 ggttcgccga tgaagtgcac cttttcgagt gccagaatcg gctgatcgtg gaaggccggc
     6841 ccgaaatagg gctgaggaat tatcttcttg atgtcttcgc cggtgatgac ggcatacacg
     6901 ccgtccattt cggccgccgc gacggtgtca atgctgataa tccgtccatg agccaccgtg
     6961 ctgcggaaga tcttcgcgtg cagcatgccg ggcacgcgca tattgtggac atagtccgcg
     7021 cggcccgtga ccttcgagcg ggcttccagg cgcgccatgc tgcgtcccat ctgattcatg
     7081 cgagcgcctc gcggatcgcg cgcgccagat agacccggag caattccttc ttgtaggacg
     7141 ccgaaccatg cgcgtcggag acaaatcttg cttcggcggc ggcgcattcg ccggcgcggg
     7201 caatcgtcgt cttgtcggac acggcgttgc tcagcagctt ttcggtcgcg gcgagacgag
     7261 tcaatctttc ggcggcggcg ccaatcacga tccgcgcccg cgcgagcgtt ccggcatcgg
     7321 ccatttcaag cttcacgccg atgttgagcg ccggccaatc gtcggccgag cgcgacgtca
     7381 ctttgacata agcgcatctg gcgccgttca acggcgggag aatcgcctcg gcgatcaact
     7441 cgttgttctt gatgaccgtt tgataataac ccaaataaag atcttcaacc gcgatttcgc
     7501 gcgtcgcctc cggtccgatc agcgaaaccc gcccccccag catcgcgaga agcggcggca
     7561 gatccatgtg gggatcgcca tggccgagcg ccccacccac gcgcgcaaca ttccgtacac
     7621 ggatattaga caaggtgcgg agcgcacctt tgatcaccgg aaaatgcgcg gccgcttcgt
     7681 gcgagttctc taattgcgcc aacgatacca tggagccgat gcgcaagctc ccgtcgccgt
     7741 tcaagcgaag catggcgtaa tcggagtcga ttctggcaag actcacgagc cgcgacggca
     7801 cgaaaaagcc cgtcttcatc atcagcatca gggccgtacc gccggcgatc gggcggaccg
     7861 cgggatcttc cggatcgagc agcgaaaccg cctccttcag gctttccggt tccacccagt
     7921 cgaacgggat caccgcatcg gcccgatctg gatgacgact aagctatcgg ccttcctgcg
     7981 cgcgccaaaa atcatgaacg gcctcaatcc gtctttcgct tgcccagacc caattccgcc
     8041 caacgcgccg tgaccgcgtc cacgtcggcc tcagccggcc acacggtcgg tggaaaccgc
     8101 atgccgccga aatcggggtc gggatcgtaa tcgaggttga tggtcgcatc gatcaacacg
     8161 cgattccatt tgccgctgcc gaacagcgcg atgttgcggt cgcgtttgcg cgtggaagga
     8221 tcgagccctg cgccgaatgt cgaaggcatg atgacgatgt cgttctcgcc tgcgttcacg
     8281 cgataggcga tggcccaatc gaccgccgcg taatcatgga tgtcgatgtc gtcgtccacc
     8341 acggtgacat gcttgtagcg gacgtgggag gccgagcttc cccagatcgc attggctacc
     8401 tgcttggcct ggttgcgata ggtctgcttg atctggacgt tgagattcat cgtgccatgc
     8461 accggcggac cccagacatc ggtgacgccg ggaatgccgg cgcggtcgag cacgttccag
     8521 gcaatcgccg agcgcatgat cgagctgcag atggcgtttt ccgaatagct cttcggcagc
     8581 gcgccttcga ccgtgccgcg caggatggga tcgttgcggt gggtgatcgc cgtcacccgg
     8641 atggtgggac gcggaagccg ctcgccggca atatagccgg tgaattcggc gaacggtcct
     8701 tcgggcata
//
LOCUS       DASYEG010000263         3298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_676957, whole genome shotgun sequence.
ACCESSION   DASYEG010000263 DASYEG010000000
VERSION     DASYEG010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676957"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /gene="nuoF"
                     /locus_tag="VGM72_11325"
     CDS             complement(<1..1117)
                     /gene="nuoF"
                     /locus_tag="VGM72_11325"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEY4265899.1"
                     /translation="MLADKDRIFTNLYGFHDWGLQAARKRGWWDGTKAILEKGPEGIV
                     DLMKKSGLRGRGGAGFPTGLKWSFMPKEVKERPHYLVVNADESEPGTCKDRDIMRHDP
                     HSLIEGCLIASFAMRAHVCYIYIRGEFLREREVFQAAIDQAYEAKLIGKNNIHGWDFD
                     LYLHHGAGAYICGEETALLESLEGKKGMPRLKPPFPANVGLYGCPTTVNNVESIAVAP
                     EILRRGPEWFTSIGRPNNSGTKVFCISGHVNKPCNVEEAMGIPMRELIDKHAGGVRGG
                     WDNLLAVIPGGASVPLIPKSICDTVLMDFDALKEVQSGLGTAAVIVMDKSTDVVKAIA
                     RLAAFYQHESCGQCTPCREGCGWMRRVMDRMVIGDAVT"
     gene            complement(1117..1716)
                     /gene="nuoE"
                     /locus_tag="VGM72_11330"
     CDS             complement(1117..1716)
                     /gene="nuoE"
                     /locus_tag="VGM72_11330"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005724.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEY4265900.1"
                     /translation="MLRRLAEHQPESFAFSAENAAWCAEQMKKYPPGRQASAVIPILW
                     RGQEQEGWVTKPMIEEVARILGMAPVRVLEVATFYTMFNLNPVGVHLVQVCTTTPCWL
                     RGSDDVVAACKKHIHPHQGAISPDGTFSWVEMECLGACVNAPMILIDKDPFEDLDGPK
                     MEAILETIRRGEQPKVGPQNGRRSSEPEGGATTFNEVQS"
     gene            complement(1726..2061)
                     /locus_tag="VGM72_11335"
     CDS             complement(1726..2061)
                     /locus_tag="VGM72_11335"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY4265901.1"
                     /translation="MSDAALLVQRQLDAYNAQDLDAYAGCYAPDVIVAGLNGAVTETG
                     RDALRARYAKAFATFPENRARLVNRIALGNTVIDHEDVSRGPGKEQFEIIAIYTVKDG
                     KIARVDFAK"
     gene            complement(2058..3248)
                     /locus_tag="VGM72_11340"
     CDS             complement(2058..3248)
                     /locus_tag="VGM72_11340"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEY4265902.1"
                     /translation="MNDVTAEADARTFSINFGPQHPAAHGVLRLVLELDGEIVTRVDP
                     HIGLLHRGTEKLIEHKTYMQAVPYFDRLDYVAPMNQEHGFALAGEKLLGIAVPPRAQY
                     IRVLYCEIGRLLNHLLQVTTQAMDVGALTPPLWGFEEREKLMVFYERASGSRMHANYF
                     RIGGVHQDLPEQLLADIHEFCRHFPKVLDDIEVLLTENRIFKQRNVDIGIISRNDALA
                     WGMSGVMLRSTGLAWDLRRAQPYECYSDLAFKIPIGKNGDNYDRYLMRMEEMREANKI
                     MIQCIEKMPKGPVTVQDGKVVPPKRGEMKRSMEALIHHFKLYTEGFHVPAGEVYAAVE
                     APKGEFGVYLVSDGSNRAYRCKIRAPSFPHLQAMDFMCKGHMLADVSAVLGSLDIVFG
                     EIDR"
BASE COUNT          641 a         1044 c          947 g          666 t
ORIGIN      
        1 gcgtgaccgc atcgccgatc accatgcgat ccatgacccg gcgcatccag ccgcaaccct
       61 cacggcaggg cgtgcattgg ccgcaggatt catgctgata gaacgcggcc aaacgcgcga
      121 tggccttcac cacatcggtc gatttgtcca tcacgatgac ggccgccgtg ccgagacccg
      181 attgcacttc cttcagggca tcgaaatcca tcagcacggt gtcgcagatc gatttcggaa
      241 tcagcggcac cgaggcgccg ccgggaatga ccgccagaag attgtcccag ccgccgcgca
      301 cgccgccggc atgtttgtcg atcagctcgc gcatcgggat gcccatggcc tcttcgacat
      361 tgcagggctt gttcacgtga cccgaaatgc agaacacctt ggttccggaa ttgtttggcc
      421 gcccgatgga tgtgaaccat tccggaccac ggcgtaaaat ctccggcgcc accgcgattg
      481 attccacatt gttgaccgtg gtgggacaac catagagccc gacattggcg ggaaaaggcg
      541 gcttaagccg cggcattccc tttttgcctt cgagggattc caagagcgcg gtttcctcac
      601 cgcaaatata cgcgccggcg ccatgatgca gatagagatc gaaatcccag ccatggatgt
      661 tgttcttgcc gatcagcttt gcctcatagg cctgatcgat ggcggcctga aaaacttcgc
      721 gctcacgaag gaactcgccg cggatataga tgtagcaaac atgggcgcgc atcgcgaaag
      781 acgcgatcag gcacccctcg atcagggaat gcggatcgtg acgcatgatg tcgcggtcct
      841 tgcaggtgcc gggttcggac tcatcggcat tcaccacaag ataatgcggc cgttccttga
      901 cctccttcgg catgaaggac cattttagcc cggtcgggaa gcctgcgccg ccacgtccgc
      961 gcaagcccga tttcttcatc agatcgacga tgccttcggg acctttttcc aggatcgcct
     1021 tggtgccgtc ccaccagccg cgcttcctcg cggcctgaag accccaatcg tggaaaccat
     1081 agaggttcgt gaagatgcgg tctttgtcgg cgagcatcag gactgcacct cattgaacgt
     1141 cgtcgcaccg ccttcgggct ccgacgagcg gcggccgttc tgcggtccga ccttcggctg
     1201 ctcgccccgg cggatcgttt ccaggatcgc ctccatcttg ggaccgtcaa gatcctcgaa
     1261 tggatccttg tcgatcagga tcatcggcgc gttgacgcag gcgcccaggc attccatctc
     1321 gacccaggag aacgtcccgt ccggggagat cgcgccctga tgcggatgga tgtgtttctt
     1381 gcaggcggcg accacatcgt ccgagccgcg cagccagcag ggcgtcgtgg tgcagacctg
     1441 caccagatgc acgccgacgg gattgagatt gaacatcgtg tagaaggtag cgacttcgag
     1501 cacgcgcacg ggggccatgc ccagtatccg cgccacctcc tcgatcatcg gcttggtgac
     1561 ccagccttcc tgttcctgac cgcgccacag gatcgggatg accgcactcg cctgacggcc
     1621 gggcggatat ttcttcatct gctcggcaca ccaggccgcg ttctccgcgc tgaacgcgaa
     1681 gctttcgggc tgatgctcgg caagacgtct cagcattctc tttacctatt tcgcgaagtc
     1741 gacacgcgca atcttgccgt ccttgacggt gtagatcgcg atgatctcaa actgttcctt
     1801 gccggggcct cggctgacgt cttcatggtc gatgaccgta ttgcccagcg cgatgcgatt
     1861 gaccaacctc gctctgttct ccgggaatgt cgcaaatgcc ttggcataac gcgcgcgcag
     1921 cgcatcgcgt cctgtttccg tgaccgcacc gttcagcccg gcaacaatga catcgggcgc
     1981 atagcagcca gcataggcat cgaggtcctg cgcgttgtag gcgtccaatt ggcgctgcac
     2041 aagcaaagcc gcgtcgctca acggtcgatc tccccgaaca caatatcgag cgagccgagc
     2101 acggcggaga catcggccag catgtggccc ttgcacatga aatccatcgc ctgcagatgc
     2161 ggaaaagacg gcgcccgtat cttgcagcga taggcacgat tgcttccatc cgagacgaga
     2221 tagacgccga actcgccctt gggcgcttcg accgcggcat agacctcgcc cgccggcacg
     2281 tggaatcctt cggtatacag cttgaaatga tggatcagcg cttccatcga gcgcttcatc
     2341 tcgccgcgct tgggcggcac caccttgccg tcctgaaccg tcaccggccc cttcggcatt
     2401 ttctcgatgc actggatcat gatcttatta gcctcgcgca tctcttccat gcgcatcagg
     2461 taacgatcgt aattgtcgcc gttcttgcca atggggattt tgaaagccag atcggaatag
     2521 cactcatagg gctgggcgcg gcgcaaatcc caggccaggc ccgtggagcg cagcatcaca
     2581 cccgacatgc cccaggcgag cgcatcgttc cgcgagatga ttccgatatc cacattgcgc
     2641 tgcttgaaga tgcggttttc ggtcagcagg acctcgatat cgtccaggac tttggggaag
     2701 tgccggcaaa actcgtggat gtccgccaaa agctgttccg gcaaatcctg atgcaccccg
     2761 ccgatgcgga aataattggc atgcatgcgc gagcccgagg cgcgctcata gaataccatc
     2821 agcttctcgc gttcctcgaa gccccagagc gggggcgtca gcgcgccgac atccatggcc
     2881 tgcgtcgtca cctgcaagag atggttgagc agccggccga tctcgcaata gagcacccgg
     2941 atgtattgcg cgcgcggcgg cacggcgatg ccgagcagct tttctccggc gagcgcgaag
     3001 ccatgctcct gattcatcgg cgccacataa tccagccggt cgaaataggg gaccgcctgc
     3061 atataggttt tatgctcgat cagcttttcg gtgccccgat gcagcaatcc gatatggggg
     3121 tcgacgcgcg tgacgatttc gccgtccagt tccagcacca gacgcagcac gccgtgagcc
     3181 gcgggatgct gcggcccgaa attgatgctg aaggtgcggg cgtcggcttc ggccgtcaca
     3241 tcgttcattt cctgggcgtg tccttaccct tatcctcgtg ccacgcctgg atgtaggg
//
LOCUS       DASYEG010000264         3710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1405978, whole genome shotgun sequence.
ACCESSION   DASYEG010000264 DASYEG010000000
VERSION     DASYEG010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405978"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..1604
                     /locus_tag="VGM72_11345"
     CDS             180..1604
                     /locus_tag="VGM72_11345"
                     /inference="COORDINATES: protein motif:HMM:NF039598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY4265903.1"
                     /translation="MDLLRQRAWRRISHTFPERQIYIRSDGRVQFLTFSSQMQAILAC
                     ICVMFLSWVAFTSVNVLFKDRILVAKERHFQQMQASYESRIADLQLSYDELNSSLVVA
                     QDRFQAIADSFEAKQQTLAAIIEHKKELQASLGIGTPLSKAAALDVKPTQSPSIASGV
                     GGVFDPGRPNLSVIAPPPSILDAGLQQNSIASKPIDRSATAIVSHAPERATFFRGAVL
                     KLGALFHRKISANDSNHPVIKQADAQSARIVRLGLGESTLLAEATQDVNKEVSRLSRA
                     LSITGINTKTLTSRVSAAGGGTESPIPFELSTDVTDDAFGAGIADAATAMGKLHDVVN
                     ALSAVPLIAPTELGSVSSSFGARVDPFNEQLAFHSGIDFSGPKGSDVHATAPGIVVFA
                     GPKGDYGNTVEVDHGYGIRTRYGHLSKILAQVGNPVDRGAVVGRLGSTGRSTGPHVHY
                     EVWYDDAVRDPGRFIKAGRDVLKE"
     gene            1588..2112
                     /locus_tag="VGM72_11350"
     CDS             1588..2112
                     /locus_tag="VGM72_11350"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY4265904.1"
                     /translation="MFSKSKETEPVAVVPPPAPPPVKRATTTRNGGVPSIISAELIVR
                     GTLISAGDVQVDGKVDGDIRAAGLVIGDKATITGDIYADEATVRGRVEGSIRARKVQL
                     CATCHVEGNILHEALSVESGAFFEGNCRHSDNPLADAPDAASVTERKSATASSPSRPP
                     QPAPAAAPASTPAE"
     gene            2158..2823
                     /locus_tag="VGM72_11355"
     CDS             2158..2823
                     /locus_tag="VGM72_11355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4265905.1"
                     /translation="MPNTLASANVVALLASLFAQADAKDPLALATIQKEADARFGGQR
                     YHPALAPLFDSAFLPVPPEVGQFLYVLTRANRPRTIVEIGTSHGLSAIHFAAALQDNG
                     EGRLITAELSAAKVKAARANLEILGLGRWVEIREGDAFETLKTLDASIDMLFLDGWKD
                     FYLPLLKLLEPRLRPGTLVIGDDTKLFPERLADYLAYVRDPARYQSADLPIGDGVELS
                     VRI"
     gene            complement(2820..>3710)
                     /gene="prfB"
                     /locus_tag="VGM72_11360"
     CDS             complement(2820..>3710)
                     /gene="prfB"
                     /locus_tag="VGM72_11360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEY4265906.1"
                     /translation="LIELAEAENDKPMIADAENALGGLRARAEVMKLESMLSGEADAN
                     DAYLEVHAGAGGTESQDWASMLLRMYTRWADKRGYKIEYMEESEGEEAGIKSATIQIK
                     GENAYGWLKTETGVHRLVRISPFDSNARRHTSFASVGAFPVIDDRIEIDIPDKDVRID
                     VYRASGAGGQHVNKTESAVRITHLPTNIVVQCQSDRSQHKNRAAAWNMLRARIYEAEM
                     RKRAEAAQAENDAKSDIGWGHQIRSYVLQPYQLVKDLRTGMQSHAPQDVLDGALDPFM
                     AASLAQKVGGEPAGDVTDVD"
BASE COUNT          710 a         1179 c         1083 g          738 t
ORIGIN      
        1 cgggtcatgc cgacgcggtg ctgacagccg cgaaggcatt gtaatcaaga caaatctggt
       61 agacggcgaa attagcctcc tatgaaatgc caagcttccg cctaaaagct tgatctcgtg
      121 cggttaagcg agtgaaataa cctgaggcag cccgcaaggg catcgaggga agggtcataa
      181 tggatttgct ccggcagcgc gcctggaggc gcataagcca tacgtttccg gaacggcaga
      241 tctatatccg cagcgatggg cgggtgcagt tcctcacctt cagttcgcag atgcaggcca
      301 ttctcgcatg catctgcgtg atgtttctca gttgggttgc attcacatcc gttaacgtcc
      361 tcttcaaaga ccgtattctc gtggcgaagg aacgccattt ccagcaaatg caggcatcct
      421 atgaaagccg tattgccgac ttgcagcttt cctatgacga gttgaacagt tcgctggttg
      481 ttgctcagga tcgcttccag gccatcgcgg attcttttga agctaagcag cagacgcttg
      541 ccgcgatcat cgagcacaag aaggaactgc aggcttcact tggtatcggg acacccttgt
      601 cgaaggccgc agcgctggac gtgaaaccca cgcagtcgcc ttccattgcc agtggcgttg
      661 gcggggtatt cgacccgggt cgcccgaact tatccgtcat cgcgcctccg ccttccattc
      721 ttgacgcagg cttgcagcag aattccatcg cttcaaagcc gattgacagg tccgcaacag
      781 caattgtttc gcacgcaccg gagcgagcga cattctttcg aggtgcagtt ctcaagcttg
      841 gcgcactgtt ccatcgcaag atctccgcaa atgattccaa ccatccggtc atcaagcagg
      901 ccgatgccca gagcgcgcgc atcgtaagac tcggattggg ggaatcaacg ctcctcgcgg
      961 aagccacaca ggatgtaaac aaagaggtgt cgcgcctgtc gcgcgcactg agcatcaccg
     1021 gcatcaacac caaaacgctc accagtcgtg tatcggctgc gggaggcggc acggaaagcc
     1081 caattccgtt tgaactctcc accgatgtca cggacgatgc tttcggtgcg ggcatagccg
     1141 atgcggcgac cgcaatggga aagctgcatg acgtggtcaa cgcgcttagt gccgtaccgc
     1201 tcatcgcccc caccgagttg ggcagcgtaa gcagcagctt tggcgcgcgc gtcgatccct
     1261 tcaacgaaca gcttgccttt cattccggga tcgatttttc cggaccgaaa ggatcggacg
     1321 tccatgccac agcgccgggc atcgtcgtgt tcgctggccc aaaaggcgat tacggcaaca
     1381 ccgtggaagt ggatcatggt tacggcatcc gcacacgtta tgggcatttg tcgaaaattc
     1441 tggcgcaggt tggaaacccc gttgataggg gcgccgtcgt tggccgactc ggatcgacgg
     1501 ggcgcagtac gggtccgcac gttcactatg aagtatggta cgatgatgcg gtaagagatc
     1561 cgggtcgatt catcaaggca ggtcgcgatg ttctcaaaga gtaaagaaac cgaacccgta
     1621 gccgtcgttc cgccacccgc gccaccgccg gtcaagcgtg caacaacaac acgcaatggc
     1681 ggggtcccct ccatcatcag cgccgagttg atcgtgcgcg gaacgctgat ctcggcaggc
     1741 gacgtgcaag tggacggcaa agtggatggc gatatccgcg ccgccggtct tgtgatcggc
     1801 gacaaggcga ctatcaccgg cgacatctac gccgacgagg cgacagtccg cggccgcgtc
     1861 gaaggcagca tcagagcgcg caaggtacag ctgtgcgcca cctgccacgt cgagggcaat
     1921 attctgcacg aagcgctttc ggtcgaatcc ggcgccttct tcgaaggcaa ttgccggcat
     1981 tcggataatc cgctggccga cgctcccgac gccgcaagcg ttacggaacg caaatcggcc
     2041 acggcatcct cgccgagccg tcccccacag cccgcgcctg cggcggctcc tgctagcact
     2101 cctgcggagt agcctcgacg ggttacatcc agggacaatt ggtctagaac ccggagcatg
     2161 cccaacacgc ttgcatccgc caatgtcgtt gcgctgcttg cctcgctgtt tgcgcaggcc
     2221 gacgcgaagg acccgcttgc gctcgcgacc atccaaaagg aggccgacgc gcgttttggc
     2281 ggacagcgct accaccccgc gcttgcgccg ctgttcgatt ccgcgttcct tccggtcccg
     2341 ccggaggtgg ggcagtttct ctatgtgctg acgcgggcga atcgcccccg caccatcgtc
     2401 gagatcggca cgtcccacgg cctttccgcg atccactttg ccgcagccct tcaggacaat
     2461 ggcgagggcc gcctgatcac cgccgaactc tccgccgcca aggtgaaggc cgcccgcgcc
     2521 aatctcgaaa tcctcgggct cggccgctgg gtggagatcc gggagggcga cgccttcgaa
     2581 acgcttaaaa cgctggatgc gtccatcgac atgctgttcc tcgacggctg gaaggatttc
     2641 tatctgccgc tgctgaagct gctcgaaccc cgcctccgtc ccggcacgct cgtcatcggg
     2701 gatgacacca agctcttccc cgaacggctg gcggactatc tcgcttatgt gcgcgatccg
     2761 gcgcgctatc aatcggccga tcttcccatc ggcgacggcg tggaattgtc cgtccggatt
     2821 tagtccacat ccgtcacgtc gccggccggc tcgccgccca ccttctgcgc caatgacgcc
     2881 gccatgaacg gatcgagcgc gccgtccagc acatcctgcg gcgcatggga ctgcataccg
     2941 gtgcgcagat ccttcaccag ctgatagggc tgtagcacat aggagcggat ctggtggccc
     3001 cagccgatgt ccgacttggc gtcgttctcg gcctgtgcgg cttccgcgcg cttgcgcatc
     3061 tcggcttcat agatgcgcgc gcgcagcatg ttccaggccg ccgcgcggtt cttgtgctgc
     3121 gagcggtcgc tctggcattg caccacgatg ttggtgggca gatgggtgat gcgcacggca
     3181 ctttcggtct tgttgacgtg ctgtccgccc gccccgctcg cgcgatagac gtcgatgcgc
     3241 acatccttgt cgggaatgtc gatctcgatc ctgtcgtcga tcaccggaaa ggcgccgacc
     3301 gaggcaaagc tcgtatgccg ccgcgcattg gaatcgaacg gcgagatacg caccagacga
     3361 tgcacgccgg tctcggtctt cagccagcca taggcgttct cgcccttgat ctgaatggtc
     3421 gccgatttga tgccggcctc ttcgccctcg ctctcctcca tatattcgat cttgtagccg
     3481 cgcttgtcgg cccagcgcgt gtacatgcgc aagagcatcg aggcccagtc ctgcgattcg
     3541 gtgccgcccg cgcccgcatg aacttcgaga taagcgtcgt tcgcatcggc ctcgcccgac
     3601 agcatggatt ccagcttcat cacctcggcg cgggcgcgca gaccgccaag cgcattttcg
     3661 gcatcggcga tcatgggctt gtcgttctca gcctcggcga gttcgatcag
//
LOCUS       DASYEG010000265         8916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1255551, whole genome shotgun sequence.
ACCESSION   DASYEG010000265 DASYEG010000000
VERSION     DASYEG010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 8916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8916
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255551"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VGM72_11365"
     CDS             complement(<1..320)
                     /locus_tag="VGM72_11365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HEY4265907.1"
                     /translation="MENSFLVGLSQQMATNRAMEVVANNLANLSTPAFKRESVEFQEY
                     MVNVPATEAEGSGTTNVAFVLDRGVARDLSEGRFDPTGAPLDLAIAGNGYFVVQTPDG
                     ERYTR"
     gene            565..1194
                     /locus_tag="VGM72_11370"
     CDS             565..1194
                     /locus_tag="VGM72_11370"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEY4265908.1"
                     /translation="MASKAKTEAPPPEEENEGAEGGAPGEAPKKRGLAGKILGPVLGI
                     LRFANPLAILKLPLKLKLAAIAGLLVVLGGGGAAVYFFALSPGKPAEEVAAESVKTEA
                     DKLPAEKAAFFDIPDIIVNIQTPDSTPAYLKLSVALELEKVEAKAAIEPVLPRIVDQF
                     QTYLRELRVEDIRGSAGVMRLKEELLRRVNLAVAPTPVRDVLLKEMIVQ"
     gene            1207..2274
                     /gene="fliM"
                     /locus_tag="VGM72_11375"
     CDS             1207..2274
                     /gene="fliM"
                     /locus_tag="VGM72_11375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEY4265909.1"
                     /translation="MADPTDPNVAGENPGENFEASGRPEGEAAGPAERILNQDEIDSL
                     LGFDVGENEAQDKSGIRAIINSALVSYERLPMLEIVFDRLVRLMTTSLRNFTSDNVEV
                     SLDNITSIRFGDYLNSIPLPAILAVFRAEELDNYGLFTVDSNLIYSIVDVLLGGRRGT
                     AAMRIEGRPYTTIERTLVQRMIEVILNDMVQAFDPLAPVSFTLDRLETNPRFAAIARP
                     ANAAILVKLRIDMEDRGGRVELMLPYATLEPIRKLLLQQFMGEKFGRDSIWESHLATE
                     LWSTRVQIDAILDEQRLPLNRIMNLEVGQTIMLNANPDSPIELRCGGVPLLKGRMGRI
                     GSSVAVRVEGAAKRTDTSSTF"
     gene            2286..2672
                     /locus_tag="VGM72_11380"
     CDS             2286..2672
                     /locus_tag="VGM72_11380"
                     /inference="COORDINATES: protein motif:HMM:NF039465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6468 domain-containing protein"
                     /protein_id="HEY4265910.1"
                     /translation="MTALPVSLIVEITLGVLLAATLFCCMRLENRLRSLRGDQESLLT
                     TVRALNGAISAAQGSLAGLRAAAHEADETLGRRVNSARGLADELSLLTSAGERIANRM
                     ETARAPRAEPMTRAMPAFADNFRAVR"
     gene            2682..3476
                     /locus_tag="VGM72_11385"
     CDS             2682..3476
                     /locus_tag="VGM72_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265911.1"
                     /translation="MKKTSKPNAAKRTDVRLLPAVIAVGVILFALKAGGLAFPASAAP
                     APETKSAAAPSAKPAPAAPPAKPADPLAAINAALPVPAKSAKPSESPKAVDELSPDAA
                     ESGISPAEMDVLTSLADRRDALEQRQRDLDLRTNIIAATEKRVDGKIVELKSLQAKIE
                     ALLGQRDAKELEQLDGLVKVYTAMKPKDAARIFASLDDSVRLGVAGRMKPDVVAGIMA
                     ALPSEVAQKLSVELASRYKVAVPPASTAAPAPSASAAPAVPATPGG"
     gene            3484..6780
                     /locus_tag="VGM72_11390"
     CDS             3484..6780
                     /locus_tag="VGM72_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265912.1"
                     /translation="MRAIQPLCWAFALCLLASASAHAAQAPVPDTPTPAVIAKPDSVS
                     VDTAQGYARILFTFGQATPVTASIADGVLTLHLGHSVNINIDTFTERLAPYVTTGRSD
                     RDGLTYRFALKTPVGLHNSTQGNQTAVDLVPDSFKGTPPDLPPPPPPAPAKAEIPDIA
                     QLPVIKMRVGEYANYTRVVFDWPTPVIYTVYPGQGRISVRFDALAKPDFSVLQTRSPA
                     WVKAAGWHLDGNATVVDLETDAESGFKDFRNGAKVVIDVLAPKTDASAVPPRVTAPAV
                     SSPSSGPKGSDAAAATKTGSAGKTPPTPARVSPMTTEPAGPSTEMTRDGAALHFPAAH
                     GHAAAVFTRGETLWIVLDGHPALDAATLLAPLTSIIVKAQSEQSGGAAVLKLVFKAPL
                     RPSVQESEAALNVTLTTGAIAPPEPLNFTRQGADGQTTLTTPLAGAGHVIQLADADAA
                     DHLFVVPARPGKGVLTPKRFVEMEALPSAAGIGIVPFTDDLSVQVQDELVTIARPKGL
                     ALSAASGANTEPVVQMLTSKQGPAFIDFEQWAKVGSSDVIATERNLRGAVARLPESEG
                     NKARLNLARYLLAQNLAPEALGEIQIIQAVDPKLANDPGISSMKGAAQYMMGRYADAR
                     ASLSGSALGADPHAALWRGMAEAKLGDFANARHDLAMSQNVLRFYPEPLQTRARLARA
                     ETGLAQGDLASANDALDQLSPQLSPRESVEARLYETQLLAAQGHINESVARLRTLEQT
                     DYAPIAAKATFERVRIELAAKKIKPADAIKTLEQLRYRWRGDDLELATLRKLGSLYFA
                     QENWRDGLTVLRTAARNFANSELGRDAQDDMRQAFNDLFLGGKADKLRPVDALALFYD
                     FIDLTPIGREGDEMIRGLSDRLVAVDLLGPAEQLLDHQVKERLDGVARAAVATRLATI
                     YLLDHKPKEALAIINDTRQTRLPDTVNTERRLLEARALAGLKQFETAIDLIADDESPQ
                     AGRLRADISWDSGNWSVAGAKAEEVLGDRYDMSGPLLPEERALIMRAAVAYSLAGNES
                     GLDLLRERYGAKMGASPDAKAFAIVSENIDRQGVAFRDLAKQIASIDSLQAFMADFKK
                     QGAAAKTASN"
     gene            complement(6832..7308)
                     /locus_tag="VGM72_11395"
     CDS             complement(6832..7308)
                     /locus_tag="VGM72_11395"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF001938.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEY4265913.1"
                     /translation="MIKNPSDLPYRPCVGAMVLNAEGQVFVGKRVDQSIEAWQMPQGG
                     IDEGETPQETLRRELREEIGTDNVEILREHPDWLTYELPKHLLGVAWEGRYRGQRLKW
                     FALRLAGADSEINVKTPHQEFSDWKWIGIGELLPLVVPFKRDIYSTIITAFRDLTK"
     gene            complement(7363..>8916)
                     /locus_tag="VGM72_11400"
     CDS             complement(7363..>8916)
                     /locus_tag="VGM72_11400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4265914.1"
                     /translation="SEAALNDARERSEEANRAKSHFLATISHEIRTPMNGVLGMARLL
                     LETSLAPDQKTYAEAIRQSGLSLLALIEDILDFSKIESGKLVLEQSDVALRPLIEGIA
                     ELLATRAFPKGIEIATAIEANVPRQIRSDGIRLRQVLTNLVGNAVKFTEQGGVLVSAS
                     LSDEPGLGAQLLISVRDTGIGVAPEKATQIFEDFVQADSSHARRFEGTGLGLAISKRL
                     VHAMGGKIGVRPAYGRGSEFWVQLPLESAVSGKPSDPLVHRHAAIVSQSAILGAGLTL
                     QLAAAGARSTILRDLDSARRALERYDLILLDAAGGDDSLIDVKGLDTPVVALLPPDER
                     ERLTVLSERGVAAYLMKPIRQDSLETRCLAVLAGETEFAATPVQPPPERCAPSGLRIL
                     LAEDNPVNALLARELLRRRGHRVEAVTNGEAAVLACSLGRFDVVVMDVHMPGLDGIEA
                     TLRIRRAETQTGTNPVPIFALTADALETGRRACLEAGMNGFLTKPVDPADLDAMLAAI
                     KPPIILAAE"
BASE COUNT         1749 a         2870 c         2670 g         1627 t
ORIGIN      
        1 cgcgtgtagc gttcgccatc gggcgtctgc accacgaaat agccattgcc cgcgatggcg
       61 agatcgagcg gcgcccccgt gggatcgaag cgtccttcgg agagatcgcg ggcaacgccg
      121 cggtcaagca caaaggccac attggtggtc ccactgcctt cggcctccgt tgccgggaca
      181 ttgaccatgt attcctggaa ctcgacggat tcgcgcttga atgcaggcgt tgaaagattg
      241 gcaagattgt tcgccacgac ttccatggcg cggttggtcg ccatttgctg ggagaggcca
      301 acaagaaaac tgttttccac ggacgttcgc ccccttctgg agcgcccttc tgatccttcg
      361 ccgctctgaa gtgcacgagc cgtgccatgc ggaaaaagcg ggcatgcgtg cggattttgg
      421 gccgggcggg acgcgggaca ggcaagcatc gcccgctcga aacggacgca gggcggcaaa
      481 tttttcccaa gcccaaacca gtccttaacc aagcgcttct aagttcagcc tcgcatttac
      541 ctatcgacga ccgccgaggc agccatggcc agcaaagcaa agaccgaagc acctccaccg
      601 gaggaagaga acgagggcgc ggagggcggt gcgcccggcg aggccccgaa aaagcgcggg
      661 ctggccggaa agatcctcgg gcccgttctg ggcattctcc gttttgccaa tccgcttgcg
      721 atcctgaagc tgccgctaaa attgaagctt gccgcgattg ccggtctgct ggttgttctg
      781 ggcggcggtg gcgcagccgt ctattttttc gccctttcgc cgggcaaacc cgccgaggaa
      841 gtcgccgccg agagcgtcaa aaccgaagcc gacaaattgc cggccgaaaa agcggctttc
      901 ttcgatattc ccgacatcat cgtgaatatc cagacaccgg attcgacgcc tgcctatctc
      961 aaattgtccg tggcactcga gttggagaag gtggaggcca aggccgccat cgagccggta
     1021 ctgccgcgca tcgtcgatca gttccaaacc tatctccgcg aactgcgcgt cgaagacatc
     1081 cgcggttcgg cgggcgtcat gcgcctcaag gaggagctgt tgcgacgggt gaatctcgct
     1141 gtcgctccca cgccggtccg tgacgtgctg ctcaaagaga tgatcgtaca atgagaagac
     1201 tccaccatgg ctgaccccac cgatccaaac gtagcaggcg aaaaccccgg ggagaatttc
     1261 gaggcgtcag gccgccctga aggggaggcc gccggtccgg cggagcgcat cctcaatcag
     1321 gatgagatcg attcgctcct tggcttcgat gtgggcgaaa acgaggcgca ggacaaatcc
     1381 ggcatccgcg ccatcatcaa ttcggcgctc gtttcctacg aacgtctccc gatgcttgaa
     1441 atcgttttcg accggctagt ccggctgatg acgacgtcct tgcggaactt cacctcggac
     1501 aatgtcgagg tcagcctcga caacatcacc tcgatccggt ttggcgatta tctcaattcg
     1561 atcccgctgc cggcgattct cgcggtcttc cgggccgagg aactggacaa ttacgggctt
     1621 ttcacggtcg attcgaatct catctattcc atcgtcgatg tcctgctcgg cggccggcgc
     1681 ggcaccgcag cgatgcgcat cgaggggcgg ccctacacca cgatcgaacg caccctggtg
     1741 cagcgcatga tcgaggtgat ccttaacgac atggtgcagg ccttcgaccc gctggcgccg
     1801 gtgagcttta cattggaccg cctggaaacc aatccgcgct ttgcggccat cgcgcgtcct
     1861 gccaatgccg ccatcctggt caagcttcgc atcgatatgg aagaccgcgg cgggcgtgtc
     1921 gaattgatgc tgccctatgc cacgctcgaa cccatccgca aattgctgct ccagcaattc
     1981 atgggcgaaa aattcggtcg cgactcgatt tgggagagcc atctggcgac cgagctgtgg
     2041 tccacgcgtg tccagatcga cgccatcctg gacgaacagc gcctgccatt gaaccgcatc
     2101 atgaatctag aggtcggcca gaccatcatg ctgaacgcga accccgattc ccccatcgaa
     2161 ctgcgctgcg gcggcgtgcc gctgctgaag gggcgcatgg ggcgcatcgg atcatcggtc
     2221 gcggttcgcg tagaaggcgc ggccaagcgc accgacacct ccagcacttt ttgaggatcg
     2281 ccattatgac tgcacttcct gtctcattga tcgtcgaaat cactcttggc gtgctgttgg
     2341 ccgcaacgct cttctgctgc atgcgcctcg aaaatcgcct gcgtagtctg cgcggcgatc
     2401 aagaaagtct tctgaccacg gttcgcgcgc tcaacggcgc gatctccgca gctcagggga
     2461 gccttgccgg cttgcgcgcc gccgcccatg aggcggacga aaccttggga cgcagggtca
     2521 attcggcgcg cggcctggcc gacgaattgt cgctcctcac cagcgcgggc gagcgcatcg
     2581 caaatcgcat ggaaaccgcc cgcgcacccc gggccgaacc catgacgcgt gcgatgccgg
     2641 ctttcgccga caatttccgc gccgtgcgct gagagggcag gatgaagaaa accagcaaac
     2701 ccaacgccgc gaaacgcacc gacgtgcgcc ttctgcccgc cgtcatcgct gtcggggtca
     2761 tcctctttgc cctcaaggcc ggcggtctcg ccttccccgc gagcgcggct cccgcgccgg
     2821 agacaaaatc cgccgccgcg ccgtcggcca agccggcgcc tgccgcgccc cctgccaagc
     2881 cggccgatcc gcttgccgcc atcaatgccg ccctgcccgt accggccaag agcgccaagc
     2941 cgtctgagag tcccaaagcg gtggacgaac tttcgcccga tgccgcggaa agcgggatca
     3001 gcccggcgga aatggatgtg ctgacaagcc tcgccgatcg ccgcgatgcg ctggagcaac
     3061 gccagcgcga tctcgacctg cgcacgaaca tcatcgcggc gaccgaaaag cgggtcgacg
     3121 gcaagatcgt ggagctcaaa tcattgcagg cgaagatcga ggcgctgctc ggccagcgcg
     3181 atgccaagga attggaacag ctcgatggac ttgtgaaggt ctacaccgcg atgaagccca
     3241 aagatgccgc gcgcatattc gcgtcgctgg acgattcggt gcgtctcggg gtcgcgggcc
     3301 gcatgaagcc cgacgtggtg gctgggatca tggccgccct tccctcggaa gtggcgcaga
     3361 aattgagcgt ggagctcgcc agccgctaca aagtggctgt tcctcccgcg tccacggcag
     3421 cgccagcacc ttctgcgtcg gccgcacccg ccgttccggc gaccccggga gggtaatttg
     3481 cgcatgcgcg ccattcagcc gctctgctgg gcttttgcgc tctgcctgct tgcaagcgcg
     3541 agcgcgcacg ctgcgcaagc cccggttccc gatacgccaa cgcctgccgt catagccaaa
     3601 cccgatagcg tgtcggtcga tacggcccaa ggctatgcgc gcattctttt caccttcgga
     3661 caagctacgc ccgtcaccgc gtccattgcc gatggagttc tgacgctcca tctcgggcac
     3721 agcgtcaaca tcaatatcga taccttcacc gaacgtctgg ctccatatgt caccacgggg
     3781 cgaagcgatc gtgacggact cacctatcgc ttcgcgctga agaccccggt gggattgcac
     3841 aactcaacgc aaggcaacca aaccgcggtc gaccttgttc cggacagttt caaaggcacg
     3901 cctcccgatc ttcctccgcc tccaccgccg gccccggcaa aggcagagat tccagacatc
     3961 gcgcaattgc ccgtcatcaa gatgcgcgtt ggggaatacg cgaattacac ccgggtggtg
     4021 ttcgactggc caacgccggt catctacacc gtctatcctg ggcaggggcg aatctcggtc
     4081 cggttcgatg cgttggccaa gcccgatttt tccgtcttgc agacacggtc gcccgcctgg
     4141 gtgaaagccg caggctggca tctggatggc aatgccaccg tggtcgacct cgagacagat
     4201 gcggaatccg gattcaagga tttccggaac ggcgccaaag tcgtgatcga cgtgctggct
     4261 ccaaagaccg atgcctccgc agttcctccg cgcgtcaccg cgccagcggt ttcttcaccg
     4321 agctccggac cgaaggggtc ggatgccgcc gccgcgacga agaccggaag cgcgggaaaa
     4381 acgcccccca cgcctgcgcg cgtatcgcca atgaccacag aaccagccgg tcccagtacc
     4441 gagatgaccc gcgatggcgc ggccctgcat tttcccgccg cccatgggca cgccgcagcc
     4501 gtattcaccc gcggcgaaac cctctggatc gtgctcgacg gccatccggc gctggatgcc
     4561 gcaacattgc tggcgccctt aacatccatc atcgtgaagg cccagagcga acaatccggc
     4621 ggcgccgcgg tgctgaagct tgtcttcaaa gctccgctcc gtccgtctgt ccaggaaagc
     4681 gaagccgctc tcaatgtcac cttgaccacc ggggcgatag cgccacccga acctctcaat
     4741 ttcacgcgtc aaggcgcgga tggccaaacg actctgacca cgccgctcgc gggcgcgggg
     4801 catgtcatcc agctcgcgga tgcggacgcc gccgatcacc tcttcgtggt tccggcgcgg
     4861 ccaggcaagg gcgtactgac gcccaagcgt ttcgtcgaga tggaggcgct gccgagtgcc
     4921 gccggcatcg gtatcgttcc gtttacggac gatctgtcgg ttcaggtgca ggacgaactg
     4981 gtgacgatag cacggcccaa gggacttgcg ctttctgccg cctcgggcgc caacaccgaa
     5041 cctgtcgtgc agatgttgac gagcaaacaa ggtccggctt tcatcgattt cgaacaatgg
     5101 gcaaaggtgg gtagcagcga tgtcatcgcc acggagcgca acctccgcgg cgccgttgcc
     5161 cgtcttcctg aaagcgaagg caacaaagcg cggttgaatc tcgcgcgcta tctgctggcc
     5221 cagaatctcg cacccgaagc gctcggcgaa atacagatca tccaggctgt cgatccgaag
     5281 ctcgcgaacg atcccggcat ctcctcaatg aagggcgccg cacaatatat gatgggccgt
     5341 tacgcggatg cgcgtgcttc gctttcaggt tccgcgctcg gggccgatcc tcacgctgcg
     5401 ctgtggcggg gcatggcgga agcgaagctc ggcgatttcg ccaacgcgcg tcacgatctc
     5461 gcgatgtctc aaaacgtttt gcgcttttat cccgaaccct tgcagacgcg cgcgcggctc
     5521 gcccgtgccg agacaggcct tgcccagggc gacctcgcca gcgcgaatga cgcgctcgat
     5581 cagctttccc cgcaattgtc gccgcgcgaa tcggtggaag cgcgtcttta tgagacccaa
     5641 ctccttgccg cgcagggaca catcaacgaa tccgttgcgc ggctgcgcac tctggagcaa
     5701 accgattacg cgccaatcgc agcgaaagcc acgttcgaac gggtgcggat cgaacttgcc
     5761 gccaagaaga tcaagcctgc cgacgcgatc aaaacgcttg agcaattgcg ctatcgctgg
     5821 cggggggacg acctcgaact cgccaccttg cgcaaattgg gaagcctgta cttcgcgcaa
     5881 gagaattggc gcgacggtct gacggtgctg cgcaccgccg cgcgcaattt cgccaattcg
     5941 gagttggggc gcgatgccca ggacgatatg cgccaagcct tcaacgatct tttcctcggc
     6001 ggcaaggcgg acaaactgcg ccccgtcgat gcgcttgcgc tattctacga tttcatcgat
     6061 ctgacgccca tcgggcgcga gggcgacgag atgatccgtg ggctgagcga tcggttggtg
     6121 gccgtcgatc ttctgggtcc ggcagagcaa ttgctggatc accaggtgaa agaacgtctg
     6181 gacggcgtcg cgcgggcggc cgtggcaacc cggcttgcga cgatctacct gctcgaccac
     6241 aagcccaagg aggcgcttgc gatcatcaac gacacccgtc aaacgcggct ccccgatacg
     6301 gtcaataccg agcgcaggct cctggaagcg cgggctctcg ccgggctgaa gcaattcgaa
     6361 accgccatcg acctgatcgc cgatgatgag tcgccgcagg cgggccgttt gcgcgccgat
     6421 atttcctggg attccgggaa ttggtccgtc gccggcgcga aggcggagga ggtcctggga
     6481 gaccgatacg atatgagcgg gccgcttttg cccgaagaac gcgcgctcat catgcgagcc
     6541 gccgtcgcct attctctggc aggcaatgaa agcgggcttg acctattgcg tgagcgttac
     6601 ggcgccaaga tgggcgcgag tccggatgcg aaagccttcg caatcgtaag cgagaacatc
     6661 gaccggcaag gcgtcgcatt ccgcgatctt gcgaaacaaa tcgccagtat cgactccctg
     6721 caggctttta tggccgattt caagaaacag ggcgccgcgg cgaaaaccgc cagcaattga
     6781 gtgccggaaa ttgggcaccg gcaggccgcg cgcgtctgcg cagcgacacc gttatttcgt
     6841 caaatcgcgg aatgccgtga taatcgtgga atagatgtcg cgtttgaatg gcaccacgag
     6901 gggcagaagc tcgccgattc cgatccattt ccaatcggaa aactcctggt gtggcgtctt
     6961 cacgttgatc tcactgtcgg cgcctgcgag ccgtagcgcg aaccatttca gcctttgccc
     7021 gcgatagcgt ccttcccacg ccactcccaa aagatgtttc ggcagttcat aggtcaacca
     7081 gtcgggatgc tcgcgcagga tttcgacatt gtcggtgccg atctcttctc taagctcgcg
     7141 ccgcagggtc tcttgcggcg tttcgccttc atcgatgccg ccttgcggca tctgccaggc
     7201 ctcaatgctc tgatcgacgc gtttgccgac gaagacctgg ccctcggcat tgagcaccat
     7261 ggctccgacg caaggccgat agggaagatc ggaaggattt ttgatcatct ggagagtgaa
     7321 gcacggccga ccggtccacg gaaagcattt ccgcgcctct gcttattccg ccgcgaggat
     7381 gatgggcggc ttgatggccg ccagcatggc gtccagatcg gcgggatcga caggcttggt
     7441 gaggaacccg ttcatgcccg cctccaaaca agccctgcgc ccggtttcga gcgcgtccgc
     7501 cgtgagcgcg aaaatcggaa ccgggtttgt tccggtttgc gtttccgcgc ggcggatccg
     7561 caatgtcgcc tcgatgccgt caagtccggg catatgcaca tccatgacga ccacgtcgaa
     7621 acgtccgagg gagcaagcca ggacggccgc ttctccattc gtaacggcct ccacgcggtg
     7681 cccacgccgg cgcagcaact cccgcgcaag caaggcgttc accggattgt cctccgcgag
     7741 cagaattcgg agcccgcttg gggcgcatcg ttcgggcggt ggttgcaccg gagtggcggc
     7801 gaactcggtc tctccggcta gaacggccag acatcgcgtc tccagcgaat cctgacggat
     7861 cggtttcatc agataggcgg caacacccct ttcagagagg acggtcagcc gctcgcgttc
     7921 gtccggcggc agaagcgcca ccaccggcgt gtcgagacct ttcacgtcga ttagagaatc
     7981 gtcacctccg gccgcgtcca gaagaatgag gtcgtatcgc tcaagcgccc ggcgcgcgga
     8041 atccagatcg cgcaaaatcg tggatcgagc ccctgccgcg gcaagttgca gcgtcaggcc
     8101 cgcacccaat atcgcagact gcgaaacgat cgcggcatgc cgatgcacga gcggatcaga
     8161 gggcttgccg gacactgcgg attcgagtgg caattgtacc caaaactcgc tgcctcggcc
     8221 atacgccgga cggacaccga ttttgccgcc catcgcatga acgaggcgct tggagatggc
     8281 gagccccagt ccggttccct cgaaacggcg cgcatgcgag gaatcggcct gcacgaaatc
     8341 ttcgaagatc tgcgttgcct tctcgggcgc cacaccgatg cccgtatcgc gcaccgatat
     8401 aagcaactgg gcacccagcc cgggttcatc cgataaagac gccgaaacaa ggacgccgcc
     8461 ctgttcggtg aacttcaccg cattgccgac aagattcgtc agcacctgac gaagccttat
     8521 cccgtcagac ctgatctgcc gggggacatt ggcctctatg gcggtcgcaa tctcgatccc
     8581 cttgggaaac gcgcgcgtgg cgaggagttc ggcaatgcct tcgatcaacg ggcgaagcgc
     8641 gacgtcactc tgctccagaa cgagcttgcc ggattcgatc ttggaaaaat ccagaatgtc
     8701 ctcgatcaag gcgagcagcg atagacccga ttggcggatc gcttcagcat aggttttctg
     8761 atcgggtgca agagacgtct ccagcaacag ccgcgccatg ccgagcacgc cattcatggg
     8821 cgtgcggatc tcgtggctga tggtggcaag aaaatgagac tttgcgcgat tcgcctcttc
     8881 ggagcgttcg cgggcatcgt tgagagctgc ctcgct
//
LOCUS       DASYEG010000266         7714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4303278, whole genome shotgun sequence.
ACCESSION   DASYEG010000266 DASYEG010000000
VERSION     DASYEG010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 7714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7714
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4303278"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3470)
                     /gene="dnaE"
                     /locus_tag="VGM72_11405"
     CDS             complement(<1..3470)
                     /gene="dnaE"
                     /locus_tag="VGM72_11405"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY4265915.1"
                     /translation="MGVERVTAKYGHANFVHLRVRSIYSLLEGAVQPKELAKLAREMH
                     MPAAAVTDTNNLFGVFEIADTLAKDGVQPIVGVTLSVDFEPPAAPHQNQQTRQSYPSV
                     AVLVKDADGYVQLSKLLSSAYLDVGPGELPHANAERLAAHAAGLILLTGGPNGPVNRL
                     LAEGQPQAAEAVLDRLAAAFGDRLYVELQRHGLPNETSVEERLIDFAYAKNLPLVATN
                     DAHFGREDMREAHDALFCIADGTFLDVENRRRLTPEHRFKTADEMAALFSDLPEAIHN
                     TIEIARRCAFRPKKRAPILPQFVPESGLAPAEEMRAQAEAGLKRRLAEHGLFAEEKVY
                     WDRLAFELNVIVGMGFPGYFLIVSDFMKWTRANGIPVGVRGSGATSLVAWALDITNLD
                     PIRFGLVFERFLNPERISMPDFDIDFCQERRDEVVRYVQAKYGRDRVGQIIALGSLQA
                     RAAVRDVGRVMQLPFGLVDRLAKLIPNPPGKHVTLADAIVSEPRLSQAAEQEEQVRRL
                     FETVGKIEGFYRHASTHPAGVVIGDRPLDEIVPLYRDPRSEMPVTQFDYEDAEKAGLV
                     KFDFLGLKTLTVIAKTEELLKARGIALDTQRIDFGDKPTFEMLSRGECVGVFQLESQG
                     MRDLIKKLRPDHINDLVALVALYRPGPMDSIPKYIACKHGREKPDYLHPSLEPILSET
                     FGVMTYQEDVMRIARELAGYSMGEADLLRRAMGKKIASEMAQHRERFISGGNERGISA
                     NVSEQIFEQAAKFAGYGFNKGHAAAYAQVAYQTAYLKANYPVEFLAASMTLDTGNTDR
                     LNIFRQEATRLGIEVRAPDVNRSAAFFACDAGRTGGAAGNGGVIYYALAAVKNVGRQA
                     MDHVVAIRESDGPFRSIADFARRIDPRLVNKRAFESLVRAGAFDALHKNRRQLVQSAD
                     IILGDAARNMRDRDVGQSSLFGAAETARETLPLPAAEDWLAHERLAEEFSAIGFYLSG
                     HPLDGYAQSLKRLGVARYADLMADARRSSVKATLAGTVIRRQERRGKSGDPFAFIGLS
                     DPSGMFEVMVFSEALTNARPFLDVGRSILLRVVGDWIDDELKLRTLSVEDLDTAAAQA
                     GEGLKIRLSDPAPIPAIARELKVAGKGLVTLIVPGQSGNQDVEIALPKRVSVNPQLKS
                     TLAALTGIAE"
     gene            complement(3879..4586)
                     /locus_tag="VGM72_11410"
     CDS             complement(3879..4586)
                     /locus_tag="VGM72_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4265916.1"
                     /translation="MSEAPGRPVMPPLRLDRVTRIYKQAGRELVVFRDVSLLLQPGEI
                     VALVGQSGAGKSSLLHIAGLLEAPSSGEVTILGEAASQLPERRRTEIRRNALGFVYQF
                     HHLLPEFTAEENVAMPRRIAGASREESAAEAKRLLALVGLAERMDHRPAQLSGGEQQR
                     VAMARALANRPRVLLADEPTGNLDTRTADGVFEMLIALVRSEGLSALIATHNYELSNK
                     MDRTLLLDKGVLAERPR"
     gene            complement(4579..5841)
                     /locus_tag="VGM72_11415"
     CDS             complement(4579..5841)
                     /locus_tag="VGM72_11415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEY4265917.1"
                     /translation="MSRNYRPFSGFEWMVAARYLRAKRQESFISVISGFSLIGIALGV
                     ATLIIVMSVMNGFRAELLGRILGLNGHLVVQSINGGIPDYDALARRILMVPGVTTATP
                     IIEGQVLVNAEGDSVGARVRGLESKDLMNLTAVSSTLSPGTMAAFDEGDSVILGARLA
                     EQLGLVPGMMVTLIAPKGAVTPFGTTPRIKTYRVAGTFRIGMSEYDSMFVFMPLKEAQ
                     LFFDLGNSAQGIEVMVANPDDVAAWRLPIVRVAGQSARITDWQQMNSSFFGALIVERN
                     VMFMILTLIILVAALNIVSGLIMLVKDKGGDIAILRTMGATRGAVMRVFFIAGAAIGT
                     IGTISGLIIGIVFCANIESIRQFLSRITGTTLFDPTIYFLSQIPAKIDPWDVVSVVGM
                     ALSLSFLATLYPSWRAARLDPVEALRYE"
     gene            5925..7484
                     /gene="proS"
                     /locus_tag="VGM72_11420"
     CDS             5925..7484
                     /gene="proS"
                     /locus_tag="VGM72_11420"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEY4265918.1"
                     /translation="MQRALSAHRMTGERSNFPDWYQAVVREADMAEPSPVRGSMIIKP
                     WGYGVWEQIQKELDRRIKDTGHDNCYFPLFIPLSFIAKEAQHVEGFAKEMAVVTHHRL
                     KMLDGKLQPDPDAKLEEPLVVRPTSETIIGDAFSRWIKSHRDLPLLINQWANIVRWEM
                     RTRLFLRTSEFLWQEGHTAHADEADAMAETLRMLEVYRDFSETTLAMPVIAGEKSENE
                     RFPGAVNTYSIEAMMQDGKALQAGTSHYLGTHFSQAQNIRFQNEAGEHVWCHTTSWGV
                     STRLIGGVIMTHGDDDGLRLPPAIAPRQIVIVPMLRGKPEDGDVLGYCQALASALAKE
                     TAFNERIRVIVDTKDIKSADKRWNWVRRGAPLILEVGPRDVAGGNVTYMRRDQLRDGD
                     KVRSTAALREQFVAEAAQLLTAIQTRLYGEAKTRLDSNIRSDIASFDALADYFGVAGD
                     DDEAGDFKGWVRAPWSSPAGAELEAVEKKLKQLKLSIRNAPLGQSAAPLGKCIFTGAP
                     AVQEVLIARAY"
     gene            complement(7499..>7714)
                     /locus_tag="VGM72_11425"
     CDS             complement(7499..>7714)
                     /locus_tag="VGM72_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265919.1"
                     /translation="LYIDALRTGAIAVVAPFRYSAAIWASIAGFLVWGDIPDWLGVTG
                     TIFVIGSGLYTFYRELELAKARRSGTG"
BASE COUNT         1566 a         2419 c         2356 g         1373 t
ORIGIN      
        1 atttcggcga tgccggtcag cgcagcgagc gtggatttga gctgcggatt gacggaaacg
       61 cgtttgggga gcgcgatctc cacatcctga ttgccggatt gacccggcac gatgagcgtg
      121 accagtccct ttcccgcaac cttcagctcg cgcgcgatgg ccggaatcgg cgccggatcg
      181 gaaagccgga tcttgagccc ctcccccgcc tgagcggccg cagtatcgag gtcttccacc
      241 gagagcgtgc gaagtttcaa ttcatcatcg atccagtcgc cgacgacacg cagcaaaatc
      301 gaacggccga catccagaaa gggacgcgca ttggtgagcg cttcggaaaa caccatcacc
      361 tcgaacatgc cgctcgggtc ggacagaccg atgaacgcaa aaggatcgcc gctttttccc
      421 cggcgttcct gacggcggat cacggtcccg gcaagcgtcg ccttcaccga ggaacggcgt
      481 gcgtccgcca tcagatcggc gtagcgcgca acacccagcc gcttcagcga ttgcgcataa
      541 ccgtccagcg gatggccgga gagatagaat ccgatggcgg agaattcttc cgccagccgc
      601 tcatgcgcca gccaatcctc ggcggcgggc agtggcagcg tttcacgcgc ggtttctgcg
      661 gcgccaaaca gactggattg ccccacatcg cggtcacgca tattgcgcgc ggcatcgcct
      721 aggatgatgt cggccgattg caccaattga cgcctgttct tatgcagcgc gtcaaaggcg
      781 cccgcccgca ccaggctttc gaaggcgcgt ttgttgacca gcctgggatc gatgcgccgc
      841 gcgaaatccg cgatggaacg gaacggtcca tcggattcgc gtatcgcgac gacatggtcc
      901 attgcctggc gcccgacatt cttcaccgcg gcgagtgcat aataaatgac accgccgttc
      961 cctgccgcgc ccccagtccg ccccgcatcg caggcgaaga acgccgccga gcgattgacg
     1021 tcgggggcgc gcacttcgat cccaaggcgc gtggcttcct gccggaagat gttgagccgg
     1081 tcggtgttgc cggtgtccag cgtcatcgaa gcggcaagga actcgacggg ataattcgcc
     1141 ttcagatagg ccgtttgata cgcgacctgc gcataggcgg cggcgtggcc cttgttgaaa
     1201 ccataacccg cgaacttagc cgcctgctcg aatatctgct cggaaacatt tgcggaaata
     1261 ccccgctcat tgccgccgct gatgaatcgt tcgcgatgct gcgccatttc ggacgcgatc
     1321 ttcttgccca tcgcccggcg caaaaggtcg gcttcaccca tggaatagcc cgcgagctcg
     1381 cgggcgatcc gcatcacgtc ttcctgatag gtcatgacgc cgaaagtctc gctgaggatc
     1441 ggctccaggc tcggatgcag ataatccggt ttttcgcggc cgtgcttgca ggcgatgtat
     1501 ttgggtatgg aatccatcgg acccggccga taaagcgcca ccaacgcgac aaggtcgttg
     1561 atgtggtcgg gcctgagttt cttgatcagg tcgcgcatgc cctggctttc aagctggaac
     1621 acgccgacac attctccacg cgagagcatc tcgaacgtgg gcttgtcgcc gaaatcgatg
     1681 cgctgcgtgt ctagcgcgat gccgcgcgcc ttcaaaagct cctcggtttt ggcgatgacg
     1741 gtgagcgtct tcaggccgag aaaatcgaat ttgacgaggc ctgccttctc cgcatcctcg
     1801 taatcgaatt gcgtcactgg catttcggaa cgcgggtcgc gatagagcgg cacgatttca
     1861 tcgagggggc ggtccccgat gacgacaccg gcgggatgcg tggaggcgtg ccggtagaat
     1921 ccttcgatct tgccgacggt ctcgaaaagc cggcggacct gctcttcctg ttcggccgcc
     1981 tgcgaaaggc gcggttcgct gacgatcgca tcggccagcg tcacatgctt gcccggcgga
     2041 ttgggaatga gcttggccag gcggtcgacc aggccgaagg gcaattgcat cacgcgcccg
     2101 acatcgcgca ccgccgcccg cgcctgcagc gaacccagcg cgatgatctg ccccacccgg
     2161 tcgcgcccat atttcgcctg gacatagcgc accacctcat cacgccgctc ctgacagaaa
     2221 tcgatatcga agtccggcat cgaaatgcgt tcgggattga ggaagcgctc gaacaccagc
     2281 ccgaaccgga tgggatcgag attggtgatg tcgagcgccc aggcgacgag cgaggttgcg
     2341 ccggaaccac gcacgccgac cggaataccg ttcgcgcgtg tccatttcat gaaatccgac
     2401 acgatcagga aatagcccgg aaagcccatg ccgacgatga cgttcagttc gaacgccaag
     2461 cggtcccaat aaactttttc ctcggcgaac agcccatgct cggcgagccg gcgtttgagc
     2521 ccggcttcgg cttgcgcacg catttcttcg gctggcgcca aaccggattc cggcacgaat
     2581 tgcggcagga tgggcgcgcg tttcttagga cgaaaagcgc agcgccgcgc gatctcgatc
     2641 gtgttgtgaa tcgcctccgg gagatcggaa aacagtgccg ccatttcgtc cgcggtcttg
     2701 aaacgatgct ccggcgtcag acgccgccgg ttctccacat ccagaaacgt gccgtccgcg
     2761 atgcagaaca gcgcatcatg ggcctcacgc atgtcttcgc ggccgaaatg cgcatcattg
     2821 gtggcgacca gcggcagatt cttggcatag gcgaaatcga tcagtctctc ctcgacggag
     2881 gtttcgttcg gaaggccgtg ccgctgaagt tcgacatata gccggtcgcc gaacgcggcg
     2941 gccagccggt ccagcacggc ttcggcggct tgcggctgac cttccgcaag cagacgattg
     3001 accggcccgt tgggtccgcc ggtgaggagg atcagcccgg ccgcatgagc cgccagacgc
     3061 tccgcattcg catgcggcag ctcgcccggc cccacgtcca gataggcgct ggagagcaat
     3121 ttggaaagct gcacatagcc atcggcgtcc ttaaccagga ccgcgaccga cggataggac
     3181 tgccgcgtct gctggttctg atgcggcgcc gcggggggct cgaaatccac cgacagcgtg
     3241 accccgacaa tcggctggac accgtccttc gccaaggtat cggcgatctc aaagactccg
     3301 aacagattgt tcgtgtcggt caccgcagcc gcgggcatat gcatctcgcg ggcaagcttc
     3361 gccagttcct tgggctgcac cgcgccttcg agcaacgaat agatgctgcg cacgcgcaaa
     3421 tgcacgaaat tggcgtggcc atatttggcc gtgacgcgct cgacgcccat gcgttacctc
     3481 aagtcgactc gtggataagt atcggcgagc ggtagttcgc gtgcacccat tcccgcccga
     3541 tgcccacagg gaaaaagcgg gcgatacccc cctccccccc atgatctttg aaggatccgg
     3601 caaaaagccg ccgttttcct aagtttctcg caggataggt gcgaaaattt cggtgcaatt
     3661 atggtgcaat tgggtgcgat ttccccaaat ctcaagccat caaggcctga ccggcaaccg
     3721 ccagcaccaa gaccgaggcc acaaacacca gcatgctcac tgccgccact gcttcctgga
     3781 accatagagc cataatcgcc tcctgctatc gaggcaatgt tcctattatg ttctcgtccg
     3841 gcaagtcaag aacgaaacta gaacaaaaca gttggcggtt agcgcggccg ctccgccagc
     3901 acacctttgt cgagcagcag cgtacggtcc atcttgttgg aaagctcgta attgtgcgtg
     3961 gcgatcaggg cggacagccc ttcggacctg accaacgcga tcagcatctc gaacacccca
     4021 tcggcggtgc gcgtgtccag attgccggtc ggctcatcgg ccagaagcac gcgtgggcga
     4081 ttggcgagcg cacgcgccat cgcaacacgc tgttgctcgc cgcccgaaag ctgggcagga
     4141 cggtggtcca tccgctcggc caggccaacg agcgcaagca gccgcttggc ttcggcggcg
     4201 gactcttccc ggctcgcgcc cgcaatccgg cgcggcatgg cgacgttttc ttccgccgtg
     4261 aattccggca gcagatgatg gaactgatag acgaagccca acgcattgcg ccggatctcg
     4321 gtgcgccggc gctccggcaa ttggctcgcg gcttcgccta ggatcgtgac ctcgccggag
     4381 ctcggcgctt ccagcagtcc cgcaatgtgc agcagtgacg atttcccggc gcccgattgt
     4441 ccgaccagtg ccacaatctc cccgggctga agcagaagcg aaacatcgcg aaacacgacc
     4501 agctcacggc cagcctgctt ataaatgcgc gtcacgcggt cgagccgcaa cggcggcatc
     4561 accggccgcc ctggcgcctc actcataacg cagggcttcc acgggatcga gtctggccgc
     4621 acgccaggac gggtaaagcg tcgccaggaa cgacagcgac aacgccatgc caacgaccga
     4681 gaccacgtcc cagggatcga tcttggccgg tatctggcta aggaaataga tcgtcggatc
     4741 gaacaatgtc gtcccggtga tgcgcgaaag gaattgccgg atggattcga tattggcgca
     4801 gaacacgatg ccgatgatca gccccgaaat cgttccgatg gtgccgatgg ccgcgcccgc
     4861 gatgaagaag acgcgcatca ccgcgcctcg cgtcgcgccc atcgtgcgca ggatggcaat
     4921 atcgcctccc ttgtccttca ccagcatgat caggcccgaa acgatgttca gcgcggccac
     4981 caggatgatc agcgtcagga tcatgaacat cacattgcgc tccacgatca acgcgccgaa
     5041 gaagctggaa ttcatctgct gccaatcggt gatccgcgcc gattgaccgg ccacgcgcac
     5101 gatgggcaaa cgccatgccg ccacatcgtc gggattggcg accatcactt cgatgccctg
     5161 cgcgctattg ccgagatcga agaaaagctg cgcttccttc aaaggcatga agacaaacat
     5221 cgagtcgtat tccgacatgc cgatgcggaa ggtcccggcg acgcgatagg tcttgatccg
     5281 gggcgtcgtc ccgaatgggg tcaccgcgcc tttgggagcg atgagggtca ccatcatacc
     5341 cggcaccagg ccgagctgtt cggcaagccg cgcgcccagg atgacggaat cgccttcgtc
     5401 gaaggccgcc atcgttcccg gcgaaagtgt cgaggagacg gcggtcaggt tcatcagatc
     5461 cttgctttcg aggccgcgca cgcgcgcgcc cacggaatcg ccctcggcat tcaccagcac
     5521 ctggccttcg atgatgggag tggcggtggt cacgccgggc accatcagga tgcggcgggc
     5581 gagcgcatcg taatcgggaa tcccgccatt gatgctctgg accacgagat gaccgttcag
     5641 tcccaggatg cggcccagaa gctcggcgcg gaacccgttc atcacgctca tcacgatgat
     5701 gagcgtggcg acgcccagcg cgatgccgat cagcgaaaag ccggaaatga cggaaatgaa
     5761 gctctcctgc cgcttggcgc ggagatagcg cgcggccacc atccattcga agccggaaaa
     5821 gggtcggtaa ttgcgggaca taagctcttt tggtggcgaa tcgcctggga gtgttctacg
     5881 gaacgagtat tcataacacc aatgtggcga tttggagccg tgacatgcaa agagccttga
     5941 gcgcgcaccg catgacgggg gagcgttcga atttcccgga ctggtatcag gccgtggtcc
     6001 gcgaggccga catggccgaa ccctcgccgg tgcgcggttc catgatcatc aagccctggg
     6061 gctatggcgt ctgggaacaa atccagaagg aactcgaccg gcgcatcaag gacaccggcc
     6121 acgacaattg ctatttcccg ctgttcatcc cgctctcctt catcgccaag gaggcccagc
     6181 atgtggaggg cttcgccaag gagatggcgg tggtcaccca tcaccggctg aagatgctcg
     6241 acggcaaatt gcagcccgat cccgacgcga aactggagga gcccctggtg gtgcggccga
     6301 cctccgaaac cattatcgga gacgcgttct cgcgctggat caagagccat cgcgacctgc
     6361 cgctgctgat caatcaatgg gccaatatcg tgcgctggga aatgcgcacc cggctgttcc
     6421 tgcgaacctc ggaattcctc tggcaggagg gacacaccgc ccacgccgat gaggccgatg
     6481 cgatggccga gaccttgcgc atgctcgaag tctatcgcga cttttccgaa acgacgctcg
     6541 ccatgccggt gatcgcgggc gaaaagagcg agaacgagcg tttccccggc gcggtgaaca
     6601 cctattccat cgaagcgatg atgcaggacg gcaaggcgtt gcaggccggc acgtcgcatt
     6661 atctcggcac gcatttttcg caagcgcaaa acatccgctt ccagaacgaa gcgggggaac
     6721 atgtgtggtg ccacaccacg agctggggtg tttcgacacg cctgatcggc ggcgtcatca
     6781 tgacccatgg cgacgatgac gggcttcgcc tgccgccggc cattgcgccc cggcagatcg
     6841 tgatcgtgcc gatgctgcgc ggcaaacccg aagacgggga cgtgctcggc tattgccagg
     6901 cgctggcgag cgctcttgcg aaggagaccg cattcaacga acgcatccgg gttattgtcg
     6961 atacgaagga catcaaatcc gccgacaagc gctggaattg ggttcggcgc ggggcgcctc
     7021 tgatcctgga agtgggccca cgcgatgtgg cgggcggcaa tgtcacctat atgcggcgcg
     7081 accaattgcg cgatggcgac aaggtgcgtt ccacggccgc cttacgcgaa caattcgtgg
     7141 ccgaggccgc ccaattgctg accgcgattc agacgcggct gtatggcgaa gccaagacgc
     7201 ggctggattc gaatatccga agcgacatcg ccagtttcga tgcgctggcg gattatttcg
     7261 gtgttgccgg cgatgacgac gaagccggcg atttcaaggg ctgggtgcgc gcgccctggt
     7321 cgagtccggc gggtgcggaa ctggaggcgg tggagaagaa gctgaagcag ttgaagctct
     7381 ccatccggaa cgcgccgctg gggcaaagcg cggcaccgct cggcaaatgc atcttcaccg
     7441 gtgcgcccgc ggtgcaggag gtgctgatcg cgcgggcgta ctaatgtgcg ccgctgcctc
     7501 agcccgtgcc ggaccgccgt gccttcgcca attcgagttc gcggtaaaag gtgtacagcc
     7561 ccgaaccgat gacgaagatc gtgccggtca caccgagcca atcgggaata tcgccccaga
     7621 cgagaaatcc ggcgatgctg gcccatatcg cggcggaata gcgaaacggc gcaaccactg
     7681 cgatggcgcc tgtgcgcagc gcgtcgatgt agag
//
LOCUS       DASYEG010000267         3465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3577957, whole genome shotgun sequence.
ACCESSION   DASYEG010000267 DASYEG010000000
VERSION     DASYEG010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3577957"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..430)
                     /locus_tag="VGM72_11430"
     CDS             complement(158..430)
                     /locus_tag="VGM72_11430"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HEY4265920.1"
                     /translation="MSPGMRVRVREDFPPGHIRTPVYVRGKEGVVTRLFGRFPNPETL
                     ALGRDGLPLKTLYEVRFRQKDLWPDYAGAANDTLDMDIYEHWLERV"
     gene            complement(442..744)
                     /locus_tag="VGM72_11435"
     CDS             complement(442..744)
                     /locus_tag="VGM72_11435"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265921.1"
                     /translation="MRGPHDIGGLEAGAVDISPHDMSLWEKEIDALNQLLAQKGYRKT
                     DENRRAIETLGHDAYDTLSYYERWTAALARVLIDKGVLTQDEIDSRIAALREAARA"
     gene            complement(820..1674)
                     /locus_tag="VGM72_11440"
     CDS             complement(820..1674)
                     /locus_tag="VGM72_11440"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY4265922.1"
                     /translation="MDWIIPVSWSETASDMLGPATANAAYAALREHGCAILRGVFSSS
                     EGEVLHREYQSQYGAMDIHQMQEQSAKPPPSQFIEVGEARYDITVRMKGAFAAPRLLA
                     NTLLRRFLTPLLGDNMRLNSFTVVVSHPGAQLQHPHRDHGHLYPELPPDPPLPPHAIN
                     VAIPLIDVGIDTGPTGIWLGSHKWPESYIPPPEAMTTVPFQRGDCILVDYRTLHTGLP
                     NRSAVVRPIIYMTYARPWFFDECNHLGRSSLNMTLETYQALPESVRPLLLRAHLQAMR
                     TRLLSETP"
     gene            complement(1758..3095)
                     /locus_tag="VGM72_11445"
     CDS             complement(1758..3095)
                     /locus_tag="VGM72_11445"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEY4265923.1"
                     /translation="MFRIVLCRLVLFLAALMLAAEPAAAHDIPSDATVQAFVKPQGQI
                     LRVLVRLPLKTVMDVEFPHRERDFVDLARADQSLRDAATIALANNLEIYEGDALLPKP
                     RIVSVRMSLESDRSFATYEGALAHVTGPPLPDSTTIFWEQGLLDVLFEYPIQSDRSYF
                     SIHAAFDRFALKTITALQFLPPNGVDRGYALEGDAGLVRLEPNWYHAAAKFVEMGFFH
                     ILDGTDHMLFLLCLVIPFRRIGPLIPIVTAFTVAHSVTLIASAYNYAPDALWFPPLIE
                     TLIATSVFYMALENIVVLRPKRRWIITFFFGLVHGFGFSFILKNTLQFAGSHILTSLL
                     SFNVGVELGQLFVLALVVPLLNILFKYVVAERMGTIILSVIVGHTAWHWMIERFDVLR
                     QFPYPVVTVADAAIALRWLIVIVAMAAFAWLLFLATQRWAKPAANQSPESGAP"
     gene            complement(3118..>3465)
                     /locus_tag="VGM72_11450"
     CDS             complement(3118..>3465)
                     /locus_tag="VGM72_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265924.1"
                     /translation="IKEAFFETNTKNAGSVWDIAFSKDPQQKYIFMADGVNEKVKIID
                     RQTLKELTTFGDGGRQPGQFYGVHSIAIDSKGNLYTTETYEGKRLQRFLAKGMAPVTT
                     LNQGALAVWPGVK"
BASE COUNT          720 a         1008 c         1092 g          645 t
ORIGIN      
        1 cgtgatggaa tcgcggaatt cgatcatgcg ggcgatttcg tccgcagtga cgatgccctt
       61 ctcgatcaac aggtctttga tcgcgagccc aagctggcgg taataggaat ggacacgggc
      121 ctcggagatt tcgtgatcgg aatgatgctc gcccatgtca aacacgctcc agccaatgct
      181 cgtaaatgtc catgtcgagc gtgtcgtttg ccgcgcccgc ataatcgggc cacagatcct
      241 tctgcctgaa gcgcacttca tagagcgtct tcaacggcaa gccgtcgcgg cccagcgcga
      301 gggtttcggg attgggaaag cggccgaaca aacgcgtcac cacgccttcc ttgccgcgca
      361 catacaccgg cgtgcggata tggccggggg ggaaatcctc gcgcacgcgt acgcgcattc
      421 cgggtgacat cgcgttttcc atcacgctcg cgcggcctcg cgcaaggcgg cgatgcgcga
      481 atcgatctcg tcctgcgtca gcacgccctt gtcgatcagc acgcgcgcga gcgccgccgt
      541 ccagcgttcg taataggaga gcgtgtcata ggcgtcatgg ccaagtgttt cgatagcgcg
      601 gcggttctcg tcggtcttgc gatagccctt ctgggccagc aattggttga gggcatcgat
      661 ctccttttcc cacaggctca tatcgtgcgg cgaaatatcg accgcaccgg cctcaaggcc
      721 gccaatatcg tgtggaccgc gcatggtttt gttcctgtca ctcccggcct ttagcgtaac
      781 ccatccctgt gaaaccggcc aatagcccgt cagtccggtt taaggggttt cgctcaaaag
      841 ccgcgtgcgc atcgcttgca gatgagcccg caggagcagc gggcgcaccg attcgggcag
      901 cgcctgatag gtctccagcg tcatgttcag cgagctgcgc ccgagatgat tgcactcgtc
      961 gaagaaccag gggcgcgcat aggtcatgta gatgatcgga cgtacaaccg cgctacgatt
     1021 gggcaggccg gtgtggagcg tgcggtaatc caccaggatg cagtcgccgc gctggaacgg
     1081 gacggtcgtc atggcttcgg ggggcgggat gtaggattcg ggccatttgt gcgaaccgag
     1141 ccagatgccg gtcggccctg tatcgatgcc gacatcgatc agcggaatgg cgacattgat
     1201 ggcgtgcggc ggcaagggcg gatcgggggg aagctccgga tacagatgtc cgtgatcgcg
     1261 atggggatgc tggagctgcg caccgggatg ggagaccacc acggtgaaac tgttcagccg
     1321 catgttgtcg ccaagaagcg gtgtcaggaa acggcgcagc agcgtattgg cgaggagacg
     1381 tggtgccgcg aaggcgccct tcatccgcac ggtgatatcg tagcgcgcct caccgacctc
     1441 gatgaactgg ctcggcggcg gcttggccga ttgttcctgc atctggtgaa tatccatcgc
     1501 gccatattgc gattggtatt cacggtgcag cacctcaccc tcgcttgatg aaaagacccc
     1561 gcgcagaatg gcgcagccat gctcgcgcaa agccgcataa gccgcgttgg cggtcgcagg
     1621 acccagcatg tccgaagcgg tctcgctcca cgaaacggga atgatccagt ccaccgccta
     1681 ccccctcgcg gatgaatgta taatatttat gagggcaccg ccccgcccgc tgtcaagttc
     1741 gggaagaacg gcgttcctta gggggcgccg gattcggggg attggttcgc ggccggcttt
     1801 gcccagcgct gtgtcgccag gaacagaagc cacgcaaacg ccgccatggc gacgataacg
     1861 atgagccatc gaagcgcgat cgcggcatcc gccaccgtga cgaccggata gggaaactgc
     1921 ctgagcacat cgaagcgctc gatcatccag tgccatgccg tgtggcccac gatcaccgac
     1981 aggatgatgg tgcccatgcg ctcggccaca acatatttga acaggatgtt gaggagcggc
     2041 accaccagcg ccagcacgaa caattgcccc agctcgactc cgacattgaa ggagagcagc
     2101 gaggtcagaa tgtgcgagcc ggcgaactga agcgtgttct tcagaatgaa ggagaagccg
     2161 aagccgtgaa cgagtccgaa gaagaaggtg atgatccagc gtcgcttcgg gcgcagcacc
     2221 acgatattct cgagcgccat atagaagacc gaggtcgcga tcagcgtctc gatgagtggg
     2281 ggaaaccata acgcatccgg cgcataatta taagcggacg cgatgagcgt gaccgaatgc
     2341 gccaccgtga acgcggtcac gatcgggatc agtggcccga tgcggcgaaa cgggatgacc
     2401 agacacagga ggaacagcat gtggtccgtg ccgtcgagaa tgtggaagaa ccccatttcg
     2461 acgaatttcg cggccgcgtg ataccaattg ggttcgagcc gaacgaggcc ggcgtcacct
     2521 tccagcgcgt agccgcgatc cacaccgttg ggcggcaaga attgcagcgc ggtgatcgtc
     2581 ttcagtgcga agcggtcgaa cgcggcatgg atcgaaaaat acgagcgatc cgattgaatg
     2641 ggatactcga acagcacatc gagaagcccc tgctcccaaa aaatcgtcgt gctgtcggga
     2701 agcggaggcc ccgtgacatg ggcgagcgcg ccctcatagg tggcgaacga gcgatcggat
     2761 tcgagcgaca tgcgcactga cacgatgcgc ggcttgggaa ggagcgcgtc gccttcgtag
     2821 atttcgagat tgttggcgag tgcgatggtc gcggcatcgc gcaaggattg atcggcacgc
     2881 gcaagatcga cgaagtcgcg ttcgcgatgc ggaaattcca catccatgac ggttttgagc
     2941 ggcaggcgaa ccagcacgcg caaaatctga ccttgcggct tgacgaaggc ctgcaccgtc
     3001 gcgtcgctcg ggatgtcgtg cgcggccgca ggttctgcgg caagcatgag cgctgccaag
     3061 aacagcacca accggcagag cacgatcctg aacatgggcg ggggtgcgat tcgcggccta
     3121 tttcaccccc ggccagaccg ccagcgctcc ctggttcaac gtggtcaccg gcgccatacc
     3181 cttggcgagg aagcgctgga gccttttgcc ttcataagtc tcggtcgtgt agaggttgcc
     3241 cttcgaatcg atcgcgatcg agtgcacgcc gtagaactga cccggctggc gtccgccatc
     3301 gccaaaggtc gtcagctctt tcaacgtctg gcggtcgatg atcttgacct tctcgttcac
     3361 gccatccgcc atgaaaatgt atttctgctg cgggtctttc gagaaggcga tgtcccatac
     3421 cgagccggcg ttcttggtat tcgtctcgaa gaacgcttcc ttgat
//
LOCUS       DASYEG010000268         4604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4450625, whole genome shotgun sequence.
ACCESSION   DASYEG010000268 DASYEG010000000
VERSION     DASYEG010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4450625"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VGM72_11455"
     CDS             <1..793
                     /locus_tag="VGM72_11455"
                     /inference="COORDINATES: protein motif:HMM:NF044806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265925.1"
                     /translation="DAVHAAGAYFYCDGANFNAIVGRVKPGALGVDAMHINLHKTFST
                     PHGGGGPGAGPVVFSERLAPFAPLPFFVEDANGFHLIEDMEEARAADATPLGRMCAFH
                     GQMGMFVRALAYMMSHGADGLRQVAEDAVLSANYILASLKDVMSAPYEGPCMHEALVD
                     DTFLKDSGITTLDIAKRLIDEGFHPMTVYFPLVVHGAMLIEPTETESKATLDQFIAVM
                     RALAGEAKAGDAERFKAAPVLSPRRRLDETLAARKPVLRWKPVLS"
     gene            complement(891..1679)
                     /locus_tag="VGM72_11460"
     CDS             complement(891..1679)
                     /locus_tag="VGM72_11460"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY4265926.1"
                     /translation="MSMHFAFHPLYSLSGFVVGALVGMTGVGGGALMTPLLILLFGIH
                     PGTAVGTDLLYASATKTVGTAVHGVSKTVEWPVVGRLAMGSVPMTIITLLVLSRLGGQ
                     SGTVSTLITSVLATALFGTATVLIFRKQIIDRYAGYLAAIAPQRTRAMTIAMGAILGV
                     VVSISSVGAGAIGVTALILLYPQLPMARIVGSDIAHAVPLTLIAGMGHWILGLTDLGL
                     LGSLLVGSIPGIIIGSYAAPRMPDAALRYLLAGTLLLVGGRLVF"
     gene            complement(1992..2189)
                     /locus_tag="VGM72_11465"
     CDS             complement(1992..2189)
                     /locus_tag="VGM72_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265927.1"
                     /translation="MRAQNQARKYATSLAKQKKRPALEIVRSPEPDCCTLCGGVIARF
                     DMEIYLLHNRCAPCHGALEDE"
     gene            complement(2202..>4604)
                     /locus_tag="VGM72_11470"
     CDS             complement(2202..>4604)
                     /locus_tag="VGM72_11470"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265928.1"
                     /translation="DPGIQFHTTINDWHTCPAGGPIRASNPCSEYMFLDDTACNLASL
                     NLMAFRKEGMNGAKAFDVPAFEHAVRLWTIVLEISVLMAQFPSKEIALLSFDYRTLGL
                     GYANIGGLLMSAGIPYDSREGRAIAGAITAVLTGISYATSAEMAGELGAFPRFEENRD
                     CMLRVIRNHRRAAYGQMDEYEDLATLPVALDHEAVLDKELSEAAKAAWDQALTFGTAY
                     GYRNAQVSVIAPTGTIGLLMDCDTTGVEPDFALVKFKTLAGGGYFKIINRCVPEALRA
                     LGYSPDEIESIVRYAVGHGTLKGANALSHGALRAKGFGDAEIEKIESALKQAFDIRFV
                     FNKWTLGAEFCVNMLKIDPAALDAPDFDMLKTLGFAKAEVEAANLYVCGAMTLEGAPY
                     LKAAHLAVFDCANPCGRIGKRALSVESHIRMLAATQPFISGAISKTINMPNNAAVKDC
                     GDSYLLSWKLGLKANALYRDGSKLSQPLASQVLQLDEDEDEDDLLAQPQAARTTVVTE
                     RIVEKIIERIHSHERERLPHRRKSYIQKAIVGGHKVYLHTGEYEDGRLGEVFIDMHKE
                     GAAFRSLMNNFAIAISIGLQYGVPLEEFVDAFTFTRFEPAGIVIGNDSIKNATSVLDY
                     IFRELAVSYLGRHDLAHVVPAQDEDLGGGIGEGARREAMRDFAKVASTGFVRGQLNQF
                     VVVRGGNAAHKLDPQHEHAYHEEDAAEIDVTSSFAEVRALVQEAAGDALGSAVAMLES
                     RISTQGGAKTSKREVHAKRAAEARLRGYEGDACGSCGNFTLLRNGTCMKCDTCGGTSG
                     CS"
BASE COUNT          913 a         1386 c         1421 g          884 t
ORIGIN      
        1 cgatgccgtc catgcggctg gcgcctattt ctattgcgac ggcgccaatt tcaacgccat
       61 cgtcgggcgc gtgaaacccg gcgcgctcgg cgtcgatgcc atgcacatca atctgcacaa
      121 gacgttctcc acgccgcatg gcgggggcgg acccggagcc gggccggtgg tgttttccga
      181 acggctggcg cctttcgcgc cgctgccgtt tttcgtggaa gatgcgaacg gtttccactt
      241 gatcgaggac atggaggagg cgcgcgccgc cgatgcgacg ccgcttgggc ggatgtgtgc
      301 gttccatggc cagatgggca tgttcgtacg cgcgctcgcc tatatgatga gccatggcgc
      361 ggatggtttg cgtcaggtgg cggaggatgc cgtgctgtcg gcgaattata tccttgcctc
      421 gctcaaggat gtgatgagcg cgccctatga gggcccctgc atgcatgagg cgctcgtcga
      481 cgacaccttt ctgaaggact cggggatcac cacgctcgac atcgcaaagc gtctgatcga
      541 tgagggattt catccgatga cggtgtattt tccgctcgtg gtgcatggcg cgatgttgat
      601 cgaacccacc gaaaccgaat cgaaggccac gctggatcag ttcatcgccg tgatgcgcgc
      661 gcttgcgggc gaggccaaag cgggcgatgc ggaacggttc aaggctgcgc cggtgctgag
      721 cccgcgccgg cggctcgatg aaacgctcgc cgcgcgaaaa cccgtgctgc gctggaaacc
      781 ggtgctttct taaatttgcc gcgcaatccg gcggcgaacc gcccccgcgc tttgcgcgcg
      841 attttagact tcacggcgaa gccgcgaggc cacttcgccg ggattgcgct ctagaaaacc
      901 agacgcccgc cgaccagaag cagcgtaccg gcaagcagat agcgcaaagc cgcatccggc
      961 atgcgcggcg ccgcgtagct gccgatgatg atgcccggta tcgaacccac cagcagggaa
     1021 cccaaaaggc ccaggtcggt caggcccagg atccaatgac ccatcccggc gatcagcgtc
     1081 agcggcacgg catgcgcgat atctgaaccg acgattcggg ccatcgggag ttggggataa
     1141 agcaggatca gcgcggtcac gccgatggcg cccgcgccga ccgacgagat cgacaccacg
     1201 acgcccagga tcgcgcccat cgcgatggtc atcgcgcgcg tcctttgcgg agcgatggct
     1261 gcaagatagc cggcgtagcg gtctatgatc tgctttcgga agatgagaac cgtggcggtg
     1321 ccgaacaggg ccgttgcgag caccgaagtt atcagcgtac tgaccgtgcc gctttgtcca
     1381 ccgaggcgcg acaagaccag cagcgtgatg attgtcatcg gtacgctgcc catggcaagc
     1441 ctgcccacca ccggccactc cacggtcttg ctgacgccgt gaaccgcggt tccgacagtc
     1501 ttggttgcgg acgcgtagag aagatccgtc ccgaccgcgg ttccgggatg aatgccgaac
     1561 aacaggatga ggagcggggt catcagcgcg ccgcccccca cgccggtcat cccgacgagc
     1621 gcgccgacaa caaaccccga taatgaataa agcggatgaa aagcgaaatg catgctcata
     1681 cgtcaggggc gcccatctaa agctgaaaga taggcgaagc acgtccgccc gtcacgtcca
     1741 gggtggcgga atagacaacc cacctccccc gggcaagagg aatgaatcct ttcgccgccg
     1801 caccggaaaa caacaacaaa tgtgacgtgg aaaagttcag gccgaaacgc agtgatttgc
     1861 acgatattcg cgcatgtcgg ccaaaaaacg gctacgccgt ttacacagtt tgctcatgtg
     1921 ctttcatgtt gccgccggct gcggcgccga agacgcagga acgcgctttc acgaagccgt
     1981 taaggatgca tctattcgtc ttccagcgcc ccatgacagg gagcacagcg attgtgcagg
     2041 agataaatct ccatgtcgaa gcgcgcgatc accccgccac agagcgtgca gcaatcgggt
     2101 tcggggctgc gcacaatctc aagtgccggc cgcttcttct gtttggcaag cgaggtggcg
     2161 tattttcgtg cctggttttg cgcgcgcatg tttcactcct ctcaactgca accgctcgtt
     2221 ccgccgcagg tgtcgcactt catacaggtg ccattgcgca acaacgtgaa gtttccgcat
     2281 gaaccgcagg cgtcgccctc atagccacgc aggcgcgctt cggcggcgcg tttcgcatgg
     2341 acctcacgtt ttgaggtctt ggccccgcct tgcgtggaga tgcgggattc cagcatcgcc
     2401 acggcggaac ccagcgcgtc accggccgcc tcctgcacca gcgccctgac ctcggcgaaa
     2461 ctcgacgtga cgtcgatttc ggcggcgtcc tcttcatgat aggcgtgctc atgctgggga
     2521 tcgagcttgt gcgctgcatt gccgccgcgc acgacgacga attggttgag ctgcccgcgc
     2581 acaaatccgg tggacgccac tttggcgaaa tccctcattg cctcgcgccg cgcaccttcg
     2641 ccgatgccgc caccgagatc ttcgtcctgt gcgggcacca catgggcgag atcgtgccgg
     2701 cccaggtagg atacggcgag ttcgcggaag atataatcga gcacggatgt cgcgttcttg
     2761 atcgaatcat tgccgatgac gatgcctgcc ggttcgaatc gcgtgaaggt gaacgcatct
     2821 acgaattctt ccagcggtac gccatattgc agtccaatgg aaatcgcgat cgcgaaattg
     2881 ttcatcaacg agcggaaagc agcgccttcc ttgtgcatgt cgatgaaaac ttctccgagg
     2941 cggccgtcct catattcacc ggtatgcaga tagaccttgt ggccgccgac gatcgccttt
     3001 tggatgtagc ttttgcggcg atgcggcaaa cgctcgcgtt cgtgcgagtg gatgcgttcg
     3061 atgatctttt ccacgatgcg ttcggtgacg acggtggttc gcgccgcctg cggctgagcg
     3121 aggaggtcgt cctcgtcctc atcctcgtcc agttgaagca cctgtgacgc caagggctgg
     3181 gacaatttcg agccgtcgcg ataaagcgca ttggccttga gcccgagctt ccaggacagc
     3241 aggtaagaat cgccgcaatc cttcaccgcc gcattgttcg gcatgttgat ggttttggag
     3301 atggcgcccg aaataaatgg ctgggtggcc gccagcatgc ggatgtggct ttccaccgac
     3361 aacgcacgct tgccgatacg cccgcaggga ttggcgcaat cgaaaaccgc caggtgcgcg
     3421 gccttgagat agggtgcacc ttcgagcgtc atggcgccgc agacgtaaag attggctgct
     3481 tcgacttcgg ctttggcgaa gcccaacgtc ttcagcatgt cgaaatcggg cgcatcgagt
     3541 gccgccggat cgatcttcaa catgttgacg cagaattccg cgcccagcgt ccatttgttg
     3601 aagacaaagc ggatgtcgaa agcctgcttc agcgcgcttt cgattttttc gatttcggca
     3661 tcgccgaaac ccttcgccct gagcgcgcca tgggagagcg catttgcacc cttcagcgtg
     3721 ccgtggccta cggcataccg cacgatcgat tcgatctcgt ccggcgaata accaagcgcc
     3781 cgtaatgctt cggggacgca gcggttgatg atcttgaaat aaccgccgcc cgcgagggtc
     3841 ttgaatttca ccaacgcgaa atcgggctcg acgccggtgg tgtcgcaatc catcagcagg
     3901 ccgatggtgc cggtgggcgc gatcacgctc acctgcgcgt tgcgatagcc ataggcggtg
     3961 ccgaacgtaa gcgcttgatc ccaggcggcc ttggcggcct ccgagagttc cttgtcgaga
     4021 accgcttcgt gatcgagtgc tacgggaagc gtggcaaggt cctcatattc atccatttga
     4081 ccataggccg cgcggcgatg attgcggatg acgcgcagca tgcaatcgcg gttctcctcg
     4141 aagcgcggga aggcgccaag ctcgcccgcc atctccgccg aggtggcata ggaaatgcct
     4201 gtgagcaccg cggtgatggc gcctgcgatg gcgcggccct ctcgcgagtc atagggaatg
     4261 ccggccgaca tcagaagtcc gccgatgttg gcgtaaccaa ggccgagcgt acgatagtcg
     4321 aaggaaagaa gagcgatctc cttggacggg aattgcgcca tcaggacgga gatttcgagc
     4381 acgatggtcc acagccgcac cgcgtgttcg aaagcaggca catcgaacgc cttcgccccg
     4441 ttcatgcctt ccttgcggaa cgccatcagg ttcagggatg cgagattgca ggcggtatcg
     4501 tccaggaaca tgtattcgga gcagggattg gaggcacgga tcgggccacc cgccgggcag
     4561 gtatgccaat cgttgatggt ggtgtggaac tggatgccgg gatc
//
LOCUS       DASYEG010000269         4649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3436054, whole genome shotgun sequence.
ACCESSION   DASYEG010000269 DASYEG010000000
VERSION     DASYEG010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3436054"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..794
                     /locus_tag="VGM72_11475"
     CDS             216..794
                     /locus_tag="VGM72_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265929.1"
                     /translation="MRSNALLIGSFIALLAVPAMAQGNNAVAVNGTVDKFEANSISVK
                     ADDGSMQTFKLADKLLVVQNKPAKLSDIKSNDFVASAAVRKEDGKLHSTELRIFPDAM
                     RGVGEGQRPMNDARNQTMTNATVTGTAIVGGSNNMKVKFPGGESDLILDPGVPVTKIE
                     PADKSEIKTGAKVRVQGIKTAEGATINRITLQ"
     gene            complement(852..2276)
                     /locus_tag="VGM72_11480"
     CDS             complement(852..2276)
                     /locus_tag="VGM72_11480"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEY4265930.1"
                     /translation="MDGSRLKNFVDALWDDSVVPSLIDYIRIPNKSPGFDKDWMAHGY
                     MDAAVALMEKWAREKIANIAGATLEVVRLADRTPVIFIEIPGVGEEPVLLYGHLDKQP
                     EFTGWSEGLGPWTPVLRGDRLYGRGGADDGYAMYGALAAILALHDQNIPHARCVVLIE
                     ACEESGSFDLPFYVDHLAKRIGSPGLVICLDSGCGDYERLWLTTSLRGTAIGRLTVNV
                     LTEGVHSGDASGIVPSSFRIARMLLSRLEDEATGTVKPGGLYVDIPPARVTQAKAAAA
                     ILGDAVYSKFPFAGGTRPMADDPAEMILNRTWRPQLAVIGADGMPTPANAGAVLLPST
                     VLKISMRLPPQLDSDKAMAVMVEELTRDPPYGAQVTFKPLLPNNTGWHAPELAPWLEQ
                     SITRAGQAAFGKPMASMGEGGTIPFMAMLGEKFPKTQFVITGVLGPNSNAHGPNEFLH
                     IPTGKRVSETIARVLADHLEQACR"
     gene            complement(2278..3267)
                     /locus_tag="VGM72_11485"
     CDS             complement(2278..3267)
                     /locus_tag="VGM72_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY4265931.1"
                     /translation="MQTRKLGPFDVSAIGLGCMVLSHAYGNPPDPKTAGEVLLKALDV
                     GYTHFDTATLYGFGVNETLLGNVLKSRRKDYVLASKCGMFRGASGTKEIDGHPAVLRQ
                     NLEDSLKRLQTDVIDLYYLHRWDKRVPIEDSIGAMADMVKAGKIRSVGLSEVSAATLR
                     KAHAVHPIAALQSEYSLWTRNPEIAALEACRELGVAFVAFSPLARGFLTGDLRDVASL
                     PPKDIRLAMPRFQSAHYPANLKLLDGFAALARENDCTMAQLALAWLLAKAPHIIPIPA
                     TTRLDHLAENAGAADVRLGPAAMARMEALINPSTISGPRYNAAQLPEIDTEGD"
     gene            complement(3334..4290)
                     /locus_tag="VGM72_11490"
     CDS             complement(3334..4290)
                     /locus_tag="VGM72_11490"
                     /inference="COORDINATES: protein motif:HMM:NF040065.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4265932.1"
                     /translation="MKICVAGQGAFGQKHLDALKRIPGVEVTSLIGGNPKGTEEVAAK
                     YGVKHWGNDLAEGIARADAVILATPTQMHRAQGEQVMRAGKHVLIEIPIADNVEDSEA
                     LARIAKETGVVAMGGHVRRFNPSHQFVHRRITKGELAIQQMDVQTYFFRRTNMNAAGK
                     PRSWTDHLLWHHACHTVDLFHYQTGETATECYAVQGPRHPQLGIAMDMGILLKVPSGA
                     ILTLSLSFNNDGPIGSFFRYIGDTATYQAYYDDLADGKKNPIDLSQVDVSMDGIELED
                     REFLSAIAEKREPNSSLAQVLPCMRVLGKLESIVDPKRECVM"
     gene            complement(4311..>4649)
                     /locus_tag="VGM72_11495"
     CDS             complement(4311..>4649)
                     /locus_tag="VGM72_11495"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265933.1"
                     /translation="AIRKAVESFDRDLRVVIFGTGGMSHQLQGPRAGLINKEFDNRFL
                     DNLTRDPAALVRIPHLEYLRDTGSEGIEMVMWLIMRGALDDHVEEVYRFYHVPASNTA
                     LGHIILENKG"
BASE COUNT          917 a         1456 c         1372 g          904 t
ORIGIN      
        1 agcgagagcc gcaaccccca gaaacagatt gttgcgcatg atgcgttacc cccctaaatc
       61 cgtgaagaac cgccagaatg ctatgaatat ttcgcgccag caagggcggc tgagggctcc
      121 tggcgtgcgg tcttgtgcga gactttgcga tctgtgctaa agagacgctg gtgcgcgaaa
      181 cgcgccgccc cccggaactt caaggaacca agaccatgcg atccaacgct cttctgatcg
      241 gaagttttat cgcgctcctc gccgttccgg cgatggcgca aggcaacaac gccgtcgccg
      301 tcaacggcac ggtcgacaag ttcgaggcca attcgatttc cgtcaaggcc gacgatggct
      361 cgatgcagac cttcaagctc gcggacaagc tattggtcgt ccagaacaag ccggccaagc
      421 tctccgacat caagtcgaac gatttcgtgg cttcggccgc cgttcgcaag gaagacggca
      481 agctgcattc gaccgaattg cgcatcttcc ccgatgcgat gcgcggcgtc ggcgaaggcc
      541 agcgtccgat gaatgacgcc cgcaaccaga ccatgaccaa cgccaccgtc accggcacgg
      601 ccatcgtcgg cggcagcaac aacatgaagg tgaagttccc gggcggcgag tccgacctga
      661 tcctcgaccc cggcgtgccc gtcaccaaga tcgagcccgc ggacaagagc gagatcaaaa
      721 ccggcgccaa ggttcgcgtc cagggcatca agacagccga aggcgcaacg atcaaccgta
      781 ttacgctcca gtgagtttca cgtaattgtt tagaaagcgg cgctctgatg gggcgccgct
      841 tttgttttgg gctagcggca ggcctgttcc aaatggtccg ccagtacgcg cgcgatggtt
      901 tccgaaaccc gcttgcccgt cggaatgtgc aggaattcgt tcgggccatg cgcgttggag
      961 ttgggaccaa gcaccccggt aatgacgaac tgcgtcttgg gaaatttctc ccccagcatc
     1021 gccatgaagg ggatcgtgcc gccctcgccc atagaggcca tcggcttgcc gaatgcggct
     1081 tggccggcgc gtgtgatgga ttgttccagc cacggcgcca gctccggcgc atgccagccc
     1141 gtgttgttcg gtagcaaggg cttgaacgtc acttgcgcgc catagggtgg atcgcgcgtc
     1201 agctcttcga ccatcaccgc catcgcttta tcgctgtcga gttgcggcgg caggcgcatc
     1261 gagattttca gaacggtgga cggcagcagc accgccccgg cattggccgg tgtgggcatg
     1321 ccgtccgcgc cgatgaccgc gagctgcggc cgccaggtgc gattaaggat catctccgcg
     1381 ggatcgtcgg ccatcgggcg cgtgccgccc gcgaagggga atttcgaata gaccgcatcg
     1441 cccaatatgg cggcagccgc cttggcctgg gtcacgcgcg cgggcggaat atccacataa
     1501 agcccgcccg gcttgaccgt tccggtcgct tcgtcctcca gccgcgaaag cagcatccgc
     1561 gcgatgcgga agctcgacgg aacgatgccc gaagcatccc ccgaatggac accctccgtc
     1621 agcacgttga cggtcagccg gcctatggcg gtgccgcgca acgaggtggt gagccagagc
     1681 cgctcataat cgccgcagcc cgaatcgaga cagatcacaa ggcccggact gccgatgcgt
     1741 ttggccagat gatcgacata gaacggcaga tcgaagcttc cggattcctc gcaggcctcg
     1801 atcagtacga cgcaacgcgc gtgcggaata ttctgatcgt gtagcgccag aatggcggcc
     1861 agcgcgccat acatggcata gccgtcatcc gccccgcccc ggccatagag ccggtcgccg
     1921 cgcagaaccg gcgtccaggg gcccaagccc tccgaccagc cggtgaattc cggctgcttg
     1981 tcgagatgac cgtagagcag aaccggctct tcgcccacgc cggggatttc gatgaagatc
     2041 acgggcgtgc ggtcagccag ccgcaccacc tccagcgtgg cgcccgcgat gttcgcgatc
     2101 ttttcccgcg cccatttttc catcagcgcc acagccgcat ccatatagcc gtgggccatc
     2161 cagtctttgt cgaaaccggg cgatttgttc ggtatacgga tataatcgat caacgagggg
     2221 acaaccgaat catcccacag cgcatcgacg aaattcttca gcctggagcc gtccaattca
     2281 gtctccttcc gtgtcgattt cgggaagctg tgccgcattg tagcgcggcc cgctgattgt
     2341 ggatggattg atcagcgcct ccatgcgggc catggcggca ggccccaggc gcacatcggc
     2401 agcgccggca ttctccgcca gatgatccaa acgcgtggtg gccggaatgg gaatgatgtg
     2461 cggggccttc gccagcagcc aggccagcgc caattgcgcc atggtgcaat cgttttcgcg
     2521 cgcaagggcc gcgaaaccat ccagcaattt cagattcgcc gggtaatgcg ccgactggaa
     2581 acgcggcatc gccaggcgaa tatcctttgg cggcaggctc gcgacatcgc gcagatcccc
     2641 cgtcagaaag ccgcgcgcca aggggctgaa cgcaacgaac gcgacaccca attcccggca
     2701 cgcctctagc gcggcgattt cgggattgcg ggtccagagc gaatattcgc tctgcaatgc
     2761 ggcaatcgga tgcacggcgt gggctttgcg caacgtcgcg gccgagactt ccgacagccc
     2821 gacgctcctg atcttgcccg ccttcaccat gtcggccatc gcgccgatgc tgtcttcgat
     2881 tgggacgcgc ttgtcccagc ggtggaggta gtaaagatcg atcacatcgg tctgcaggcg
     2941 cttcaggctg tcctcgagat tctgcctgag cacggcgggg tgcccgtcga tttcctttgt
     3001 ccctgaagcg ccgcggaaca tcccgcattt gctggccagc acataatcct tgcggcgcga
     3061 cttcagaaca ttgcccaaca gcgtttcgtt gaccccgaaa ccatacaagg tcgccgtatc
     3121 gaaatgggtg tagccgacat cgagcgcctt cagcagaact tcgccagcgg tcttcggatc
     3181 gggcggattg ccataggcat gcgacagcac catgcagccg agaccgatgg cggaaacatc
     3241 aaacggacca agttttcttg tctgcatatt tctctcgtgc gctttgtgcg tccttcgtgt
     3301 tcttcgtgtc tcgtgcatcg aaggaacaca aaattacatg acgcattcgc gtttgggatc
     3361 gacgatgctt tcgagtttcc cgagcacgcg catgcaaggc agcacctgcg ccagactgga
     3421 attgggctcg cgcttttccg cgatggcgct gaggaattcg cgatcctcca gctcgatccc
     3481 atccatcgac acgtcgacct gcgacagatc gatgggattt tttttgccgt ccgcgagatc
     3541 gtcgtaatag gcttgatagg tggccgtgtc cccgatatag cggaagaacg aaccgatcgg
     3601 cccgtcattg ttgaacgaca acgacagcgt gagaatagcg cccgagggca ctttcaggag
     3661 aatgcccatg tccatggcga tgccgagctg gggatggcgc ggtccctgta cggcatagca
     3721 ttcggttgcg gtttcgccgg tctggtaatg gaacagatcg accgtatggc aggcgtggtg
     3781 ccacaggaga tgatcggtcc agctgcgtgg ttttcccgcg gcgttcatat tggtgcgccg
     3841 gaaaaaataa gtctgcacat ccatttgctg gatcgccagc tcacccttcg tgatgcggcg
     3901 atgcacgaat tggtggctgg gattgaagcg gcgcacatga ccacccatgg ccacaacgcc
     3961 ggtctccttg gcgattctcg ccagcgcttc ggaatcctcg acattgtcgg cgatgggaat
     4021 ctcgattagc acatgcttgc ctgcgcgcat tacttgctcg ccctgcgcgc gatgcatctg
     4081 ggtcggtgtc gccagaatca ccgcgtccgc acgcgcgatg ccttcggcca gatcgttgcc
     4141 ccaatgtttg acgccgtatt tggccgcgac ttcctcggtg cctttgggat tgccgccgat
     4201 cagggaggtg acctccacgc cggggatgcg cttcaacgcg tccagatgtt tctgaccgaa
     4261 cgcaccctga ccggccacgc aaatcttcat cgctcaaccc ttcccgattc tcaacctttg
     4321 ttttccaaga tgatatgccc aagcgcggtg ttcgacgccg gcacatgata gaagcgataa
     4381 acctcctcca cgtgatcgtc gagcgcaccc cgcatgatca gccacatcac catttcgatg
     4441 ccttccgatc cggtgtcgcg cagatattcg agatggggaa tgcgcactag cgccgcggga
     4501 tcgcgcgtca gattgtcgag gaagcgattg tcgaattcct tgttgatgag cccggcgcgc
     4561 ggcccctgca attgatgcga catgccgccg gtgccgaaga tcaccacccg caaatcgcgg
     4621 tcgaagcttt ccaccgcctt gcggatggc
//
LOCUS       DASYEG010000270         5953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_390257, whole genome shotgun sequence.
ACCESSION   DASYEG010000270 DASYEG010000000
VERSION     DASYEG010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390257"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2241
                     /locus_tag="VGM72_11500"
     CDS             <1..2241
                     /locus_tag="VGM72_11500"
                     /inference="COORDINATES: protein motif:HMM:NF016944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEY4265934.1"
                     /translation="IAAYKTFIGALLDLTDNIGPGGSVISPAHTLRYDGDDPYFVVAA
                     DKGTATFSDIANGIALSRSFWLGDAFASGGSQGYDHKKMGITARGAWEAIKRHFRELS
                     RDIQTEPFTCVGVGDMSGDVFGNAMLCSRATRLVAAFDHRHIFIDPTPEPLDSFAERE
                     RLFALPRSSWADYDAKLISKGGGVFARSAKEVPLSEEMRQLTGLAKDRATPSELIRAL
                     LTAEVDLLFFGGIGTYIKSAAQSHGDAGDRTNDGLRVNGRDIRARVVGEGANLGVTQL
                     GRIEYARDGGLGHKGGRIDTDAIDNSAGVDTSDHEVNLKILMSGPLRRGELSQDERLS
                     VLSAMTEDVARLVLADNYDQTLALSVAESTAARDSDAHTRFMRELERNGALDREVEFL
                     PADDVMQARQREGHGLTRPELCVILAYAKLDLYHAINESELAADPYFDAVLTGYFPSL
                     AAARFQTELKRHRLARDIVATELANRTVNLAGPLFAHRMRELSNAPIWCTARGFALAE
                     GTFGLSQLKTRICALDLKVPAATQNAMMADIAELLRRLGLWFIVQLPHAPIAETVARY
                     GAGFAALKGRFAGLVSPIEAEGVELRIASFTKAGVPKDLAEDIAVLPLLAAIPEIVLL
                     QEAQNVPAETAGRVYFAVGSLVGLDRLRALAARIAAGDHWDRLALRRIMDDLFSAQRL
                     LACDALARGRGQPGQEAVDGWAKLREADIARTVSFLSELERGGEASIAKLALANSQIQ
                     KLASPA"
     gene            2281..3891
                     /locus_tag="VGM72_11505"
     CDS             2281..3891
                     /locus_tag="VGM72_11505"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEY4265935.1"
                     /translation="MLAAVICGNYPLPLRVPAPMSQEIEIRFAASERELKRIARMSAL
                     EGFEVGRATTRRLCTVYYDTPELSLSKAGISLRVRKNGREYVQGVKDESSGALASERH
                     EVESEIVSGQPDLASVPDEALRARLQALTATLAITPVIETEIQRTTRSMKSPAGDAVE
                     LAVDRGEIRTLMNGHARLAVSEIELELKGGAPAALYEIARRLSRRSPLTVNIESKSAR
                     GMRALEGEQICAHKAGRTQLPSDCTAEEAFRLTLLHCLRHLARNAPAVAEARLPEGVH
                     QVRVGLRRLRAALSASGGNFRVRELESLDERASNLSDIFGSTRELDVFATELLAPVED
                     SLKKPGLPQLRLLLEELRRASWEQAVKLVQSDDFTGFLIDLAAAIESRIWRESASAEQ
                     LAAFERPARALASETQDKRLKQACKRAKHLSSLNVQERHRLRIALKKLRYSAEFFAPL
                     FAAKPVSAFLERLSKLQDLFGALNDAATVETILRRITEHAGKRSSAELLEAAAFVDGW
                     HQSRVEPTWDKAKKRWKIFIKTEPFWRS"
     gene            complement(3888..4691)
                     /gene="xth"
                     /locus_tag="VGM72_11510"
     CDS             complement(3888..4691)
                     /gene="xth"
                     /locus_tag="VGM72_11510"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY4265936.1"
                     /translation="MPQIKLATWNINSVRLRAGLVGQFLKNHAPDVLCLQETKVPDPL
                     FPGEIFRDLGYVHQAINGQKGYHGVAILSRLPFRTTHAMAFCGKDDTRHVSVEFETGL
                     ELHNFYVPAGGDIPDPKANPKFAHKLDFLREMESCFAKRGRQKSDPTVLVGDLNVAPH
                     ENDVWNHKQLLDVVSHTPVETELLAAALDSFEWNDVTRGVVPPAEKIYSWWSYRARDW
                     LASDRGRRLDHIWVTRPLRDGISDHLILKEARGWLRPSDHVPVMATITF"
     gene            complement(4941..>5953)
                     /gene="tsaD"
                     /locus_tag="VGM72_11515"
     CDS             complement(4941..>5953)
                     /gene="tsaD"
                     /locus_tag="VGM72_11515"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY4265937.1"
                     /translation="ASVVRGSAPGPGTILSNIVFSQLDAHRPYGGVVPEIAARAHVER
                     LDGIVEEALSTAGVALADLDAVAATSGPGLIGGVMVGLTTAKALSLAASKPLVAVNHL
                     EAHALTARMTDGAAFPFLLLLISGGHCQLLGVAGVGEFKLYGTTIDDAVGEAFDKTAK
                     LLGLSYPGGPQIEILAQSGNATRFALPRPMLGRPGADFSFSGLKTAMRLAVGEQTRLT
                     PQDRSDLAAGFQAAAVAVLIDRVRNAMNIFRTEFGITNEYRNFVAAGGVAANGPIRQA
                     LGDLARQEGFVLSLPPGALCTDNGAMVAWAGLERLSLGLTDGLDVPPLARWPLQSLYQ
                     RD"
BASE COUNT         1124 a         1873 c         1845 g         1111 t
ORIGIN      
        1 atcgccgcct acaagacctt catcggcgcg cttctggacc tcaccgacaa tatcggtccc
       61 ggcggcagcg tcatctcccc cgcccatacg ctgcgctacg atggcgatga tccctatttc
      121 gtggtcgccg ccgacaaggg caccgccact ttttccgata tcgccaacgg catcgcgctc
      181 tcgcgcagtt tctggctcgg cgatgccttc gcgtcgggcg gcagtcaggg ttacgaccac
      241 aagaagatgg gcatcaccgc ccgcggcgcg tgggaagcga tcaagcgtca tttccgcgag
      301 ctttcacgcg atatccagac agagcccttc acctgtgtgg gcgtcggtga catgtcgggt
      361 gacgtcttcg gcaacgcgat gctctgctcg cgcgcgacaa ggctcgtcgc ggccttcgat
      421 caccggcaca tcttcatcga ccccacgccg gagccactgg actcgtttgc ggaacgcgag
      481 cgcctgttcg cgctgccgcg ttcctcctgg gccgattacg atgcgaagct gatttccaag
      541 ggtggtggcg tctttgcgcg aagcgccaag gaggtgcccc tgtccgagga gatgcgccag
      601 ctcaccggac ttgccaagga tcgcgccacg ccgtcggagc tgatccgcgc tcttctgacc
      661 gccgaggtcg atcttttgtt cttcggcggc atcggtacct acatcaaatc ggcggcgcaa
      721 tcgcacggcg acgcaggcga ccggaccaac gatggcttgc gcgtgaacgg ccgcgatatc
      781 cgggcccgtg tcgtcggtga aggcgccaat ctcggcgtga cccaattggg ccgcatcgaa
      841 tatgcgcgcg acggcggcct cggccacaag ggcggacgca tcgataccga cgccatcgac
      901 aattcggcag gcgtcgacac gtccgaccac gaggtcaatc tcaaaatcct gatgagcgga
      961 ccgctgcggc ggggcgagtt gagccaggat gagcgccttt cggtgctaag cgccatgacc
     1021 gaagatgtgg caaggctggt gctcgcggac aattatgatc agactctcgc cctttccgtc
     1081 gccgaatcca ccgcggcgcg tgacagcgat gcgcatacgc gtttcatgcg cgagctggaa
     1141 cgcaacggcg cgctcgaccg cgaggtcgag tttctgcccg ccgatgatgt gatgcaggcg
     1201 cggcagcgcg aagggcacgg tctcacgcgg cccgagctgt gcgtgatcct cgcctatgca
     1261 aagctcgatc tgtatcacgc gatcaacgaa agcgagctgg cggccgatcc gtatttcgac
     1321 gcggttctga ccggctactt cccgtccttg gcggcagcgc gcttccaaac cgagttgaag
     1381 cgccaccggc tggcgcgcga catcgtcgcc accgaacttg ccaatcgcac cgtcaatctc
     1441 gcggggcccc tcttcgcgca ccgcatgcgc gagctttcca atgcgcccat ctggtgtacg
     1501 gcacgcggct ttgcgctcgc cgagggcacc tttgggctct cgcaactgaa gacgcgcatt
     1561 tgcgccctcg acctgaaggt gcccgccgcg acccagaacg cgatgatggc cgacatcgcc
     1621 gagcttttgc ggcgtctcgg actgtggttc atcgtccaac tgccccacgc cccgatcgcc
     1681 gagaccgtcg cgcgctacgg cgccgggttc gccgcgctca aaggccgttt cgcagggttg
     1741 gtctcgccta tcgaggcgga aggcgtggag ttgcggatcg cgagtttcac gaaggccggt
     1801 gtgcccaagg atctggcgga agatatcgcg gttctcccgc tgctcgccgc gattccggaa
     1861 atcgtcttgt tgcaggaagc tcaaaacgtg ccggcggaaa ccgcgggccg ggtttatttc
     1921 gccgtcggct cgttggtggg cctcgaccgc ttgcgggcgc tggcggcgcg gattgcggcg
     1981 ggcgatcatt gggatcgtct ggcgctgcgc cgcatcatgg acgatctttt ctcagcccaa
     2041 agactgctgg cctgtgacgc gcttgcccga ggccggggac agccgggcca ggaagcggtg
     2101 gatggctggg cgaagctccg cgaagcggac atcgcaagga cggtgagctt ccttagcgaa
     2161 ctggaacgcg gcggcgaagc gtcgatcgcg aagctcgcgc tcgccaacag ccagattcaa
     2221 aaactcgcct caccggcctg agccgtcgca ccccgtacaa aacaggaacg gtggccgtga
     2281 ttgctcgccg ccgtcatttg cggcaattat ccgctgccct tgcgcgttcc cgctccaatg
     2341 tcccaggaaa tcgaaatccg ttttgccgcg tccgaacgcg aactcaagcg cattgcgcga
     2401 atgtccgcat tggaaggttt cgaagtcggc cgcgcgacaa cacgcaggct ctgcaccgtc
     2461 tattacgaca cgccggagct ttcgctttcc aaggcgggga tttcattgcg cgtgcgcaag
     2521 aatggccgcg agtatgtgca gggtgtcaaa gatgaaagtt ccggcgcgct cgcttcggaa
     2581 cgtcatgagg tcgagagcga gatcgtttcc ggccagcccg atcttgcctc ggttcccgat
     2641 gaggctttac gggcccggct gcaagcgctc acggcaacgc ttgcgatcac accggtgatc
     2701 gagaccgaga tccagcgcac gacgcgttcg atgaaaagcc cggcaggcga tgccgttgaa
     2761 cttgcggttg accgcggcga aatccgcacc ttgatgaacg gtcatgcgcg ccttgcggtc
     2821 agcgaaatcg aactcgaact gaagggcggt gcaccggcgg ccctctacga gatcgcgcgc
     2881 cggctgtcgc gccgaagtcc gctcaccgtc aacatcgaaa gcaaatcggc acgcgggatg
     2941 cgcgcgctcg aaggcgaaca gatttgcgcc cataaggcag gccggaccca attgccgtcc
     3001 gactgcacgg cggaggaggc tttccgcctg accttgctgc attgcttgcg ccatctcgcc
     3061 cgcaacgcgc ccgcggtcgc cgaagcacgg ttgccggagg gcgttcacca ggtccgcgtg
     3121 gggcttcgcc gcttgcgggc ggctctttcg gcctccggcg ggaatttccg tgtgcgcgaa
     3181 ctggagtcct tggacgaacg ggccagcaat ctttccgata ttttcggcag cacacgcgaa
     3241 ctggatgtgt tcgcgaccga attgcttgcg cccgtagagg acagcctcaa gaaaccggga
     3301 ttgccgcaac tcagattgct cctcgaagaa ctgcgccgcg catcctggga acaggccgtc
     3361 aagctggtgc aatccgacga cttcaccggg ttcctcatcg atctcgcggc ggccatcgaa
     3421 agccgcatct ggcgcgaatc ggcatccgct gagcaattgg ccgccttcga gcggcccgcg
     3481 cgcgcgcttg cctccgaaac tcaggacaag aggctgaagc aggcgtgcaa gcgcgccaag
     3541 catctgtcat cgctcaatgt gcaggagcgt catcgtttgc gcatcgcgct gaagaagctg
     3601 cgctattcgg cggaattctt cgcaccgctt ttcgccgcca agccggtttc agcctttctc
     3661 gagcgcctga gcaaattgca ggatctcttc ggtgcgctca acgacgcagc gaccgtcgag
     3721 acgatcctgc gccgcatcac cgagcacgcg ggcaaaagga gcagcgcgga attgctggaa
     3781 gcggccgcgt tcgtcgacgg ctggcatcaa agccgggtcg aaccgacctg ggacaaagcg
     3841 aagaagcgct ggaagatttt tatcaaaacc gaacccttct ggcggtccta gaacgtgatc
     3901 gttgccatga ccggcacgtg gtcggaagga cgcagccagc cgcgcgcttc tttcaggatc
     3961 aagtgatccg agatgccatc gcgcaacggc cgcgtgaccc aaatatggtc aagcctgcgg
     4021 ccacggtccg aagccagcca gtcgcgcgcg cgataactcc accaggaata gattttctcc
     4081 gcaggcggca cgaccccgcg cgtgacatcg ttccattcga agctgtccag ggcggcagcg
     4141 aggagttcgg tctcaaccgg cgtgtggctg accacatcga gtagctgctt gtggttccag
     4201 acatcattct catgcggagc cacgttgaga tcgccgacca gcaccgtggg gtcgctcttc
     4261 tgccggcccc gtttggcgaa gcaggattcc atttcgcgca gaaaatcgag cttatgggcg
     4321 aatttcgggt tggccttcgg atcggggata tcgcctcccg ccggcacata aaaattgtgc
     4381 agctcaagtc ccgtctcgaa ttcgaccgag acatgacgcg tgtcgtcctt gccgcaaaaa
     4441 gccatcgcat gggtcgtgcg gaatggcagg cgcgaaagga tggccacccc gtggtaacct
     4501 ttctggccat tgatcgcctg atggacatag ccgaggtccc ggaaaatctc acccgggaac
     4561 aggggatctg ggaccttggt ctcctgcagg caaagcacgt ccggtgcatg gtttttcaga
     4621 aactgcccga ccaggccagc gcgcagccgc accgagttga tgttccaggt cgcgagtttt
     4681 atttgaggca tgtcattgtt tattaacgat ctctgtttat atatattgca gtgcaacacg
     4741 tatgttgcta tgcacaatca gatattggag agtatggtta tgacggaagt gacgttgcgg
     4801 gctgttcatc ccgcggagag cagagagatt gtgtccttga gtgcgcgttt cctgtctcag
     4861 atggttatcg cagtggctgg ggttctgtcc tttgtcccct ttgcagtgta tttcgctcaa
     4921 cattgacgcg gggcggctgg ttagtctctc tgataaaggc tttgcagagg ccaacgcgca
     4981 agtgggggca catccagccc atctgtgagc ccgagagata gacgctccag gcctgcccac
     5041 gccaccattg caccattatc cgtgcaaagt gctcccggcg gaagcgaaag aacgaaccct
     5101 tcctggcggg ccaaatctcc gagggcctgc cggatggggc cattggccgc tacaccgcca
     5161 gcagcgacga aattccggta ttcgtttgtt attccgaact ccgttcgaaa aatattcatg
     5221 gcgtttcgca ctcgatcgat gaggaccgca accgctgcgg cctgaaaacc ggcggcaagg
     5281 tccgagcggt cttgcggggt gaggcgggtt tgctcaccca ctgccaggcg catcgcggtc
     5341 ttcaggcctg aaaaggaaaa atccgccccc ggccggccca gcataggcct gggaagcgca
     5401 aagcgcgtgg cattgccgga ctgcgccaaa atctcgattt gcgggccgcc cggataggaa
     5461 aggcccagga gcttagccgt cttatcgaag gcttcgccga ccgcgtcgtc gatcgtcgtg
     5521 ccgtagagct tgaactcgcc cacaccggca acgccgagca actggcaatg cccgccggaa
     5581 atcagtaaca gcaggaacgg aaacgcggca ccatccgtca tccgcgcggt gagcgcatga
     5641 gcctcgagat gattgacggc gacgagcggt ttgctcgcgg cgagcgacag cgcctttgcc
     5701 gtcgtcagtc cgaccatcac accgccgatc aggccggggc cggaggtcgc ggccaccgca
     5761 tcgagatcgg cgagcgcaac accggccgtc gaaagcgcct cctcgacaat cccgtccagc
     5821 ctctcgacat gcgcgcgcgc ggcgatttcc ggtaccacgc cgccataggg ccggtgcgcg
     5881 tcgagctggc tgaacacgat attggaaagg atcgtgccgg gtccgggcgc ggagccgcgc
     5941 accaccgatg cgg
//
LOCUS       DASYEG010000271         7172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2566657, whole genome shotgun sequence.
ACCESSION   DASYEG010000271 DASYEG010000000
VERSION     DASYEG010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2566657"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..2017)
                     /gene="dxs"
                     /locus_tag="VGM72_11520"
     CDS             complement(98..2017)
                     /gene="dxs"
                     /locus_tag="VGM72_11520"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008035715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEY4265938.1"
                     /translation="MTVISKTPLLDEIHDPADLRALPRENLNQLAAELRQETVDVVSL
                     TGGHLGAGLGVVELTVALHRVFETPQDKIIWDVGHQSYPHKILTGRRARMRTLRQGGG
                     ISGFTKRNESPYDSFGAAHSSTSISAGVGFAVARDLAGQKHNVVSVIGDGAMSAGMAY
                     EAMNNAGAANARLIVILNDNDMSIAPPVGAMSAYLARLVSSRTYRGLRYFLKRVSQRF
                     PRFFRVAAQRTEEYARGFWMGGTLFEELGFYYVGPIDGHNLDHLIPVLENVRDNDTGP
                     TLVHVVTQKGKGYAPAEASSDKFHGVSKFNVLTGEQKKAAAGPPSYTKVFAEALIAEA
                     ERDEKVVGITAAMPSGTGLDLFAKRFPKRFFDVGIAEQHAVTFAAGLAAEGYRPFAAI
                     YSTFLQRAYDQVVHDVAIQSLPVRFAIDRAGLVGADGPTHAGAFDVAYLATLPGFVVM
                     AAADEAELMHMVATARSIDDRPSAFRYPRGEGMGVPLPKIGQPLALGKGRILREGSSV
                     AILNFGARLAECLKAAEKFSAYGLSATVADARFAKPLDTDLVRRLAQNHEVLLTVEEG
                     AIGGFGAHVLHFAAWDGLLDRGLKIRTMILPDRFLDQDTPERMYEISGLDAKGIVAAG
                     LSALGRERDAAAVRG"
     gene            complement(2104..3009)
                     /locus_tag="VGM72_11525"
     CDS             complement(2104..3009)
                     /locus_tag="VGM72_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY4265939.1"
                     /translation="MSATAPLAPIEPAFAEAASFVEATLEMLLPKPGGPELRLMEAMR
                     YAALGGGRRLRAFFVLQSGRLFGVDRRALGRVAAAVECLHAYSLVHDDLPGMDDDDMR
                     RGKPSLHKAFDEATAILAGDALLTLAFGLIASPEAHGDPFIRCELMSRFALAAGHNGM
                     VGGHMMEISLGGRDPALAEITRLARMKTAALFTFACEAGAIMGRASATARQAVTAYGQ
                     ELGLAYQIANDLFGLDGEVVDSRKDARKDATHGKAKLALFLGLERARAQAQAMAAQAT
                     AHLDLFDEKADLLREAARFVVAGMA"
     gene            complement(3022..3276)
                     /locus_tag="VGM72_11530"
     CDS             complement(3022..3276)
                     /locus_tag="VGM72_11530"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEY4265940.1"
                     /translation="MAVKEEAAALAVGDLSFEVALAELESIVSRLEQGEVDLEDSIAL
                     YERGTALKAHCDAKLKGAEMRLEKIVLGPEGAKGTEPMES"
     gene            complement(3292..4221)
                     /locus_tag="VGM72_11535"
     CDS             complement(3292..4221)
                     /locus_tag="VGM72_11535"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEY4265941.1"
                     /translation="MTTALITHPACLLHEPPPGHPERPARLSEVLKVLEGPEFGALLR
                     RAAPEADQRSLARVHDPDFVAELLEAMPQSGYAQIDSDTIASPGTAEAILRAAGAVIL
                     GVDMVLKGEAGNAFCAVRPPGHHAMPARAMGFCFFNSVAVGALHARAVHGLTHIAVVD
                     FDVHHGNGTQAMFTPDPELFYASTHQSPLYPGTGSPDETGIANNIVNAPLAPGAGGPE
                     FRRALEREILPALTAFRPELVIISAGFDAHRDDPLAELRLDESDFAWATQSLCAIAKK
                     SCGGRVVSSLEGGYDLAATARSAGAHVRALMAA"
     gene            complement(4234..5151)
                     /locus_tag="VGM72_11540"
     CDS             complement(4234..5151)
                     /locus_tag="VGM72_11540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4265942.1"
                     /translation="MPSIIVPARNNAESTARCLATILIAVNRLRLACEFVLVDDASAP
                     EEQILSVFHAHRANAAGHDTKIVRSRKHQHYSGVFSIGLHHTTRDLIFFISNDMIMTT
                     SFLQALLLVSSLSREFGIVRGTSNYTDSHPEHQVEPKETLKNYPEIDAFSRNIFGVNG
                     CNHVEDHVLSGDAILVKRALVERIGVLDLRFFGYFGDIDYGMRAHLAGFKLVCAKGAW
                     LFHEGSGHLRHQMNQGTLSFEAARDSRFDLVEKGYQEFRKKWNVTTPENWVGAQNEFR
                     FLDRARSQDGRVPLKYDFPASVLDDLEIH"
     gene            complement(5173..6342)
                     /locus_tag="VGM72_11545"
     CDS             complement(5173..6342)
                     /locus_tag="VGM72_11545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY4265943.1"
                     /translation="MQPVLLNWGVSAFSGWGIVGLNLLQHWANDPDLRVTMGAGITAS
                     ELAGLDPLRIIACREAILASNQFQSELQRVKNGTRTLSMPVMEGLGNGLYFEGGLTGT
                     RTIGRCIFEDTRLDGLDGKLAKYDLLVTASHWNAALLRANCRKPVEIVLEGIDPSLFH
                     PAPKSGLTDPGRFYIFSGGKVEFRKGHDLVLLAFREFSRRHAEATLVTLWHSPWPQLS
                     VGFKGRLDAPLGQTREGAIDVKNWVAKNGIDPAKVIEIAPLPNAMMPALLREMDCALA
                     PSRAEACTNLLAKEAMACGVPVILAPNTGVKDLIAGDNAVTLTRQTPVRDSGQMGTEG
                     WGESDVEEIVEALERLYGDSALRKKIGANGADWIVKEDRTWAHHARGLKSLLLSL"
     gene            complement(6345..>7172)
                     /gene="sppA"
                     /locus_tag="VGM72_11550"
     CDS             complement(6345..>7172)
                     /gene="sppA"
                     /locus_tag="VGM72_11550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEY4265944.1"
                     /translation="LSSAGNSVAGDIFSQAIRDAAADKNVKAILLRVDSPGGSAIASD
                     QILAALKKARAAGKPIVVSMGSVAASGGYYIAVAGDRIVAEPGTLTGSIGVLWGKVAV
                     GKSLEMVGLEGRELGVGKNALFLSSMTPWDPAQLAAVNSQADLVYADFTQKVAAGRKL
                     PLDRVQEIARGRVWTGADAKARGLVDELGGFWTAVADAKQLAHIAPGAQVAFKYYPAP
                     RGLVGAVSRLMGSTAASMKALDGLDVLMQSAPVRAMIELGRASQGGVQFRAADLPLH"
BASE COUNT         1341 a         2326 c         2246 g         1259 t
ORIGIN      
        1 acgttgcccg agcggatggc gccttgcgct tcggagcgcg tcgcggcaaa cccccggctg
       61 acgaggaaaa ggtcggcgcg ctcgctttgg tcgggcatta tccccgcacg gccgcggcgt
      121 cgcgttcgcg tccgagcgcc gaaagtccgg cggcaacaat gccctttgcg tcgagcccgg
      181 agatttcata catccgctcg ggcgtgtcct gatccaggaa gcggtcgggc aggatcatgg
      241 tgcgaatctt caagccgcgg tcgagaagcc cgtcccaggc cgcgaaatgc agcacatgcg
      301 cgccaaagcc gccaatggcg ccttcctcga cggtcagcag cacttcatgg ttttgcgcca
      361 gccgccgtac cagatcggta tcgagcggct tggcgaagcg ggcatcggcc actgtcgcag
      421 acaatccgta ggcggagaat ttttccgcag ccttcaggca ttccgcgaga cgcgcgccga
      481 aattcaggat cgcgacggag cttccctccc gcaggatgcg gcccttgccg agcgccagcg
      541 gctgcccgat cttgggcagc ggcacgccca tgccttcgcc gcgcggatag cggaaggccg
      601 acggccggtc gtcgatgctt cgcgccgtcg cgaccatatg catcagctcc gcctcgtcgg
      661 ccgccgccat gacaacgaaa ccgggcagcg tcgccagata ggcgacgtcg aacgcaccgg
      721 catgggtcgg tccgtcggcg ccgacgagac cggcgcggtc gatggcaaag cgcaccggca
      781 gcgactggat cgcgacgtcg tgcaccacct gatcgtaggc gcgctgcagg aaggtcgaat
      841 agatcgccgc gaaaggccgg taaccctcgg cggcaaggcc ggcggcaaaa gtcacggcgt
      901 gctgctccgc gataccgaca tcgaagaagc gtttgggaaa gcgtttcgcg aacaggtcga
      961 ggccggtgcc ggacggcatg gcggcggtga tgcccaccac cttctcgtcg cgctccgctt
     1021 cggcgatcag cgcttcggcg aagaccttgg tgtagctcgg gggccccgcg gcggccttct
     1081 tctgctcgcc ggtcagcacg ttgaacttcg aaacgccgtg gaatttgtcg gaggaggctt
     1141 cggccggcgc gtagcccttg cccttctggg tcacgacatg gaccagtgtc ggccccgtat
     1201 cgttgtcgcg aacattctcc agcaccggaa tcagatggtc cagattgtgc ccatcgatcg
     1261 gccccacata atagaagccc aattcctcga acagcgtgcc gcccatccag aagccgcgcg
     1321 catattcctc ggtccgctgc gcggcgaccc ggaagaagcg cgggaagcgc tgcgacaccc
     1381 gcttcaggaa atagcgcaag ccgcgatagg tccggctcga taccagccgg gcgagatagg
     1441 cgctcatggc cccgaccgga ggggcaatcg acatgtcgtt gtcgttgagg atgacgataa
     1501 ggcgcgcatt cgcggcgccc gcattgttca tcgcctcata ggccatcccg gcgctcatcg
     1561 ccccgtcgcc gatcacgctg acgacgttgt gtttctggcc cgccagatcg cgcgccacgg
     1621 cgaagccaac gccggccgaa atcgaggttg aggaatgggc ggcgccaaag ctgtcgtagg
     1681 ggctttcatt gcgcttggtg aacccggata tcccgccacc ctggcgcagg gtgcgcatgc
     1741 gtgcccggcg gccggtgaga atcttgtggg gataagattg atgaccgacg tcccagatga
     1801 ttttgtcctg cggagtctcg aaaacgcggt gcagcgcgac ggtcagttcc accacaccga
     1861 ggcccgcgcc caaatgtccg cccgtgagcg aaaccacatc gacggtttcc tggcgcaatt
     1921 cggctgccag ttgattgagg ttctcacgcg gaagcgcacg caggtcggcc ggatcgtgaa
     1981 tttcgtccag caaaggggtc ttggaaataa cggtcacgaa actctctttg aaaaagtcgc
     2041 cgcgcgaagc gtggctcgga ttgtggaggt ccctcgaaag cttagcagag cgattcgaaa
     2101 ggatcaggcc attccggcca ccacaaagcg cgctgcctcc cgcaaaagat cggctttctc
     2161 gtcgaacaaa tcaaggtgag cggtggcctg ggccgccatc gcctgggcct gggcgcgcgc
     2221 ccgttcgaga ccgagaaaca aagccagctt ggccttcccg tgcgttgcat ccttgcgggc
     2281 gtctttgcgc gaatcgacca cttcgccgtc aaggccgaaa agatcgttgg cgatctgata
     2341 ggccaggccg agttcctgac cataagccgt gaccgcctgg cgcgcggtgg ccgaggccct
     2401 tcccatgatg gcacccgctt cgcaggcgaa cgtaaacaac gcagcggttt tcatgcgcgc
     2461 caagcgtgtg atctcggcca gcgccggatc gcgcccgccg agcgagatct ccatcatgtg
     2521 gcccccgacc atgccgttgt gaccggccgc aagagcgaag cgcgacatca attcgcagcg
     2581 tataaaggga tcgccatgcg cctccggcga ggcgatcagt ccgaaggcca gcgtcaacaa
     2641 tgcatcgccg gcgagtatgg cggtcgcctc gtcgaacgcc ttgtgcagcg acggcttgcc
     2701 gcggcgcata tcgtcatcgt ccatgcccgg aagatcgtca tgcaccaggg aataggcgtg
     2761 cagacattcg acggcggcag ccacccgccc gagcgcgcgg cggtcgacgc cgaagagccg
     2821 tccgctttgc agcacgaaaa aagcgcgcag acgccggccg ccgcccaaag cggcgtagcg
     2881 catcgcttcc atgagacgca gttccggacc gccgggtttg ggcagtaaca tctccaacgt
     2941 cgcctcgacg aaggaggctg cctcggcgaa agccggttcg atcggcgcca agggcgcggt
     3001 ggcgctcatc gaaggacctt cttacgattc catcggctcc gtgcctttgg cgccctccgg
     3061 accgaggaca attttttcga gacgcatttc cgcgcccttg agcttcgcat cgcaatgggc
     3121 cttcagcgcc gtgccgcgtt cgtaaagcgc aatggaatcc tcaaggtcca cttcaccttg
     3181 ttccagccgc gaaacgatgc tctcaagctc ggccagcgcg acctcgaagc tcaaatcccc
     3241 gaccgccagc gctgctgcct cttctttcac cgccatagga gggttccttg ttcaggccgc
     3301 catcaaggca cgcacatgcg cgcccgccga acgggctgtc gcggcgaggt catagccccc
     3361 ctcgagcgag gacacaacgc gcccgccaca gctctttttt gcgattgcgc acaggctttg
     3421 cgtcgcccag gcaaaatcgc tctcgtccag acgcagctcc gccaacggat cgtcgcggtg
     3481 cgcgtcgaag ccggccgaga tgatcacaag ctccggcctg aaggcggtca gtgccggcag
     3541 gatttcgcgc tcaagggcgc ggcggaattc gggcccaccc gcgcccggcg caagcggggc
     3601 attgacgata ttgttggcga tgccggtttc atcggggctg cccgtgcccg gatagagcgg
     3661 cgattgatgg gtcgaggcat agaacagttc cggatcgggc gtgaacatcg cctgggtccc
     3721 gttgccatga tggacatcga aatccaccac cgcgatatgc gtcagcccat gcaccgcgcg
     3781 ggcatgaagg gccccgaccg cgacgctgtt gaaaaagcag aaccccatgg cccgggccgg
     3841 catcgcgtga tgacccggcg ggcgcaccgc gcaaaaggcg ttcccggctt cccctttcaa
     3901 gaccatatcg acgcccagga tgaccgcacc cgcggcgcgc agaatcgcct cggccgtgcc
     3961 cggtgacgcg atggtgtcgg aatcgatctg ggcgtaaccc gattgcggca tggcctcgag
     4021 caactcggca acgaaatccg ggtcgtggac gcgcgccagc gatctttggt cggcttcggg
     4081 tgctgcccgg cgtaacaatg cgccgaattc cgggccttcc aggactttca aaacttcgga
     4141 aagccgcgcg gggcgttcgg gatgccccgg cggaggttca tgcagcagac aggcgggatg
     4201 ggtgatgagg gctgtggtca ttggggaact cgctcaatgg atctcaagat cgtccagtac
     4261 cgaagccggg aaatcgtatt tcaacggcac gcggccgtcc tgcgaacgcg cgcgatcgag
     4321 gaagcggaat tcgttctgcg cgccaaccca attttccggc gttgtcacat tccatttctt
     4381 gcggaattcc tgatagccct tttccaccaa atcgaaccgg ctgtcgcgcg ccgcctcgaa
     4441 gctgagcgtg ccctggttca tctgatggcg caaatggccg cttccctcat ggaacagcca
     4501 ggcgcctttt gcgcaaacga gcttgaagcc cgccagatgg gcccgcatgc cataatcgat
     4561 gtcgccgaaa tagccgaaga atctgagatc gagcacgccg atgcgctcca cgagcgcgcg
     4621 tttcaccagg atggcatcgc ccgacagcac gtgatcctcc acatgattgc agccattgac
     4681 gccgaatatg ttgcgcgaga atgcgtcgat ttccggatag ttcttgagcg tttccttggg
     4741 ctcgacctga tgctcgggat gagaatccgt gtagttcgag gtgccgcgca caatgccgaa
     4801 ttcgcgcgac agcgatgaaa ccagcagtag cgcctgcaag aagctggtgg tcatgatcat
     4861 gtcgttgctg atgaagaaga tgagatcgcg cgtcgtgtga tggagaccaa tggaaaagac
     4921 gccggaataa tgctgatgct tgcgcgatct tacgatcttg gtgtcgtgac cggcggcatt
     4981 ggcgcggtgg gcgtggaaga cgctgagaat ttgctcctcc ggggcggagg catcgtcgac
     5041 caggacgaac tcgcaggcga gccggagccg gttcaccgcg atgaggattg tcgccagaca
     5101 tcgcgccgtc gattcggcat tgttacgcgc gggaacgatg atgctcggca ttaaggcggg
     5161 tccttgggcg ggttacagcg acaatagcaa agatttcaag ccgcgcgcgt gatgcgccca
     5221 ggtgcggtct tctttcacaa tccagtccgc gccattcgcc ccgatttttt tacgaagggc
     5281 actgtcgccg taaagccgtt cgagcgcttc cacgatctcc tccacatcgg attcgcccca
     5341 cccttccgtt cccatttggc cagaatcgcg gaccggcgtc tgccgtgtca gggtgacagc
     5401 attgtcgccg gcgatgaggt ccttgacccc ggtattgggc gccaagatga ccggcacgcc
     5461 gcatgccatc gcttccttcg ccagcaaatt ggtgcaggct tcggcgcgcg agggcgccag
     5521 cgcgcaatcc atctcgcgca gcagcgccgg catcatcgca ttgggcaggg gcgcgatctc
     5581 gatcaccttc gccggatcga tgccgttctt cgccacccaa ttcttaacgt cgatggcacc
     5641 ctcgcgagtc tgcccgaggg gtgcgtcgag ccgtcccttg aagcccacgg aaagctgcgg
     5701 ccagggcgag tgccagagcg tgacgagcgt ggcctccgcg tgacggcggg agaattcgcg
     5761 aaacgcgagc agcacgaggt cgtggccctt gcggaattcc accttgccgc ccgagaagat
     5821 gtagaagcgg ccgggatcgg tgaggcccga tttgggcgcg ggatggaaca gcgagggatc
     5881 gatgccttcc aagacaatct ccacgggttt gcggcaattg gcccgcagca atgcggcgtt
     5941 ccaatgcgag gccgtgacca gcagatcata tttcgcgagt ttgccgtcca gcccgtcgag
     6001 ccgtgtgtcc tcgaagatgc agcggccaat ggtgcgggtg ccggtcaggc cgccttcgaa
     6061 atagaggcca ttgccgagtc cttccatcac cggcatggat agtgtgcgcg tgccgttctt
     6121 cacgcgctgc agctcgctct ggaattgatt ggacgcgagg atcgcttcgc ggcaggcgat
     6181 gatgcgcaag ggatccagcc cggccaactc gctggcggtg atccccgccc ccatcgtcac
     6241 gcgaagatcg ggatcgttcg cccaatgctg gagcaggttc aggcccacga tgccccagcc
     6301 cgaaaacgcg ctgacccccc aattgagcag aaccggctgc atcgtcaatg cagcggcaga
     6361 tcggcggcac gaaactgcac gccgccttgt gacgcacggc ccaattcgat catcgcccgg
     6421 accggcgccg actgcatcag gacatccagc ccatcgagcg ccttcatgct cgccgctgtg
     6481 cttcccatca gccgcgagac cgcgcccacc agcccgcgcg gtgcggggta atatttgaac
     6541 gcgacctgcg ctccgggtgc gatatgggcg agctgtttcg cgtcggccac cgccgtccag
     6601 aacccgccaa gctcatcgac gagcccgcgc gccttcgcat cggcccccgt ccagacgcgc
     6661 ccgcgcgcga tctcttgcac gcggtccagc gggagcttgc gtcctgccgc caccttttgc
     6721 gtgaaatcgg catagaccag atcggcctga ctattcaccg ccgccaattg cgccgggtcc
     6781 cacggcgtca tgctcgaaag gaacagcgcg ttcttcccca caccaagctc gcggccctca
     6841 agacccacca tttcgagcga cttgccgacc gcgaccttgc cccacagcac gccgatggag
     6901 ccggtgagcg tgccgggttc cgccacgatg cggtcgcccg cgaccgcgat gtaatagccg
     6961 cccgacgccg cgaccgagcc catgctgaca acaatgggct tgccggcggc gcgtgctttt
     7021 ttgagcgcgg ccaggatttg atccgaagcg atggccgagc ctccgggcga atccacgcgc
     7081 aacaggatcg ccttcacatt cttgtcggcg gccgcgtcgc gaatggcttg ggaaaaaatg
     7141 tcgcccgcga ccgaattccc ggcgctgctc ag
//
LOCUS       DASYEG010000272         2850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3147763, whole genome shotgun sequence.
ACCESSION   DASYEG010000272 DASYEG010000000
VERSION     DASYEG010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3147763"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VGM72_11555"
     CDS             complement(<1..899)
                     /locus_tag="VGM72_11555"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY4265945.1"
                     /translation="MMNAAGRPTAPSTSDKAFVETVSGHRGLDHEEPLIFEIGRNHVM
                     GVDLPDADLTRNRLGGLARKGVMGLCGLSEPEAMRHYVRLSQRNASIDASFYPLGSCT
                     MKHNPRLDEKMARLPGFADIHPMQPQKTVQGAFALIAELARWLKELTGMNAVAMTPKA
                     GAHGELCGILAIRAALRARGENRDRILVPESAHGTNPATAALCGFGVESIRATQDGRV
                     DLTALKAALNNGVAGLMLTNPNTCGLFERDMIAIADAVHAAGAYFYCDGANFNAIVGR
                     VKPGALGVDAMHINLHKTFSTPHG"
     gene            complement(896..2236)
                     /gene="gcvPA"
                     /locus_tag="VGM72_11560"
     CDS             complement(896..2236)
                     /gene="gcvPA"
                     /locus_tag="VGM72_11560"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEY4265946.1"
                     /translation="MRYLPLTSEDRRGMLARIGAADIDGLFRDVPEAARNPQFALPGH
                     SGEMEVERTLSALAARNVAAGSSPFFCGAGAYRHHVPATVDHLIQRSEFLTSYTPYQP
                     EIAQGTLQVLFEFQTQVALITGMEVANASMYDGSTATAEAVLMAHRITGRNRALLSGS
                     LHPHYAETVRTMAGLAGEIVAAPAQPGQAEDFASLIDDTTSCVVVQNPDVFGFVRDLA
                     PIAQMAHARGALLIAVFTEAVSLGLIESPGAMDADIVAGEGQALGNSLNFGGPYVGLF
                     AAREKYLRQMPGRLAGQTVDAEGRRGFVLTLSAREQHIRREKATSNICTNSGLCALAF
                     SIHLSLLGEAGLTRLAKLNHARALELADRLTQIKGVELVTPSFFNEFTLKLPAPAAVV
                     ADALADKGVIAGVRASRLYPHESGLRNHLIVAATEMNTDADIDSYADVLAEVLA"
     gene            complement(2236..2607)
                     /gene="gcvH"
                     /locus_tag="VGM72_11565"
     CDS             complement(2236..2607)
                     /gene="gcvH"
                     /locus_tag="VGM72_11565"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEY4265947.1"
                     /translation="MEERFTESHEWIRVDGAVATIGITKYAADQLGDVVFVELPEPGK
                     TFAAGAQAAVVESVKAASEIYAPVTGEVTEANPALASDPAKVNADPEGEAWFFRMKIA
                     GDTGLTKFMTKDQYTDFVKGL"
     gene            complement(2612..>2850)
                     /locus_tag="VGM72_11570"
     CDS             complement(2612..>2850)
                     /locus_tag="VGM72_11570"
                     /inference="COORDINATES: protein motif:HMM:NF020253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEY4265948.1"
                     /translation="QAPAREGADIRDASGRVIGRVTSGGYSPSLAAPIAMGYVDTAFA
                     ADETSVSIFLRERPRAANVAPMPFVPHRYKRSGS"
BASE COUNT          524 a          914 c          878 g          534 t
ORIGIN      
        1 ccatgcggcg tggagaacgt cttgtgcaga ttgatgtgca tggcatcgac gccgagcgcg
       61 ccgggtttca cgcgcccgac gatggcgttg aaattggcgc cgtcgcaata gaaataggcg
      121 ccagccgcat ggacggcatc ggcgatcgcg atcatgtcgc gctcaaacag cccgcacgta
      181 ttgggattgg tcagcatcaa tcccgcgacg ccgttgttaa gcgcggcttt cagtgccgtg
      241 agatcgacgc ggccgtcttg cgtggccctg atgctttcca cgccgaaacc gcagagcgcc
      301 gcggtggcgg gattggtgcc gtgcgcggat tccggcacca ggatgcggtc ccgattttcg
      361 ccgcgcgcgc gcagagcggc ccgaatggcg aggatgccgc acaattctcc atgtgcgccc
      421 gctttaggcg tcatcgcgac cgcattcatg ccggtcagct ctttcagcca gcgcgccagc
      481 tcggcaatca gggcaaacgc accctgcacg gttttctgcg gctgcatcgg atggatgtcc
      541 gcgaagccag gcagccgcgc cattttctcg tccagccgcg gattgtgttt catcgtgcaa
      601 gagccgagcg gatagaagct cgcatcgatg gaggcgttgc gctgcgacag gcgcacataa
      661 tggcgcatcg cttcgggctc ggagaggccg caaagaccca tcacgccttt gcgcgcgagg
      721 ccgccaagtc gattgcgcgt gagatcggca tcgggaagat ccacgcccat cacatggttg
      781 cgcccgattt cgaagatcag cggttcttcg tgatccaggc cccggtggcc cgaaacagtc
      841 tccacgaacg ctttatccga tgtgctgggg gcagtaggcc gcccggcggc attcatcatg
      901 cgagcacctc ggccaatacg tctgcatagg aatcgatatc ggcatcggta ttcatttcgg
      961 ttgccgcgac gatcagatga ttgcgcaaac cggactcatg cggatacagc cttgaggcgc
     1021 gcacccccgc gatcacgccc ttgtccgcca aggcatcggc gaccacggcg gcgggtgcag
     1081 gcagcttcag cgtgaattcg ttgaagaagc tcggcgtcac cagttccacg ccctttattt
     1141 gcgtcagccg gtcggccagt tccagcgcgc gcgcatgatt gagctttgcg aggcgcgtca
     1201 gtcccgcctc ccccaacagc gacagatgaa tcgaaaatgc gagcgcgcag aggcccgaat
     1261 tggtgcagat attgctcgtg gctttctcgc ggcggatatg ctgttcgcgg gccgagagcg
     1321 tgagcacgaa accgcgacgt ccttcggcat ccaccgtctg acctgcgagc cggccgggca
     1381 tctggcgcaa atatttctcg cgcgcggcaa acagcccgac atagggtccg ccgaaattca
     1441 agctgttgcc aagcgcctgt ccctcccccg ccacgatgtc ggcgtccatc gcgccgggcg
     1501 attcgatgag accgagcgac acggcctcgg tgaagaccgc gatgagaagc gcacctctcg
     1561 cgtgcgccat ttgtgcaatg ggcgccagat cgcgcacaaa tccgaacacg tcgggatttt
     1621 gcaccacgac gcaggaggtg gtgtcatcga tcagcgacgc gaaatcttcc gcctgccccg
     1681 gctgcgcggg cgctgcgacg atctcccccg caagccccgc catggtgcgc accgtttcgg
     1741 cataatgcgg atgcaggctg cccgacagca acgcgcgatt gcgccccgtg atgcgatggg
     1801 ccatcagcac cgcttcggcg gtagcggtcg agccgtcata catcgaggca ttcgccacct
     1861 ccatgccggt gatgagagcc acctgcgtct ggaattcgaa cagaacctgc agcgttcctt
     1921 gcgcgatctc cggctgatag ggcgtgtagg aggtgaggaa ttccgagcgt tggatcaaat
     1981 gatccaccgt tgccggcaca tgatggcgat acgcccccgc gccgcaaaag aacgggctgc
     2041 tgccggcggc gacgtttcgc gccgccaggg ccgaaagcgt gcgttcgacc tccatctcgc
     2101 cggaatggcc gggaagcgcg aattgggggt tgcgcgcagc ttcgggcacg tcgcggaaga
     2161 gtccgtcgat atcggcagcg ccgattctgg ccagcatccc gcgccggtcc tcggacgtca
     2221 gaggaaggta acgcattaca atcccttcac gaaatccgta tactgatctt tcgtcatgaa
     2281 cttcgtcaaa cctgtatcgc cggcgatttt catccggaag aaccaagctt cgccttcggg
     2341 atcggcgttc accttggctg gatcggaggc caaagcggga ttcgcttccg tcacctcgcc
     2401 cgtcacgggg gcatagattt cgctcgccgc cttgacgctc tcgacgaccg cggcctgcgc
     2461 gcctgccgcg aacgtctttc caggctcggg caattcgacg aagaccacgt cgcccaattg
     2521 atcggccgca tatttcgtaa tgccgatagt tgcgaccgcg ccatcgacgc gaatccattc
     2581 gtggctttcg gtgaatcgct cttccatatt ttcaactccc actgcgtttg tagcgatgcg
     2641 gcacgaaggg catgggcgcg acattcgcgg cccgcggcct ctcacgcagg aaaatcgaga
     2701 cggacgtctc atcggccgcg aatgcggtgt cgacatagcc catggcaatg ggtgcggcga
     2761 ggctcgggct atagccgccg ctggtcaccc gcccgatgac gcgccccgac gcatccctaa
     2821 tgtccgcgcc ttcgcgggca ggcgcctgtt
//
LOCUS       DASYEG010000273         5844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3728197, whole genome shotgun sequence.
ACCESSION   DASYEG010000273 DASYEG010000000
VERSION     DASYEG010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3728197"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VGM72_11575"
     CDS             <1..199
                     /locus_tag="VGM72_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEY4265949.1"
                     /translation="GAGLDVFEHEPKVHPTLLGLTNVALLPHLGSSTIEARTGMGNKV
                     IANIAALVEGKPLPDGVSPPV"
     gene            372..680
                     /locus_tag="VGM72_11580"
     CDS             372..680
                     /locus_tag="VGM72_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265950.1"
                     /translation="MTQTIYRSLAAAALALSGAAALVAAAIPALAQSLPNGNGKELVA
                     TICTSCHDLSPVTEGGFSKDDWNMVIKSMIEMGADIKPDQATVLVNYLATNFPPKPKQ
                     "
     gene            complement(746..2029)
                     /locus_tag="VGM72_11585"
     CDS             complement(746..2029)
                     /locus_tag="VGM72_11585"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4265951.1"
                     /translation="MSVSRTRKFHYGWTVAAITFLILLVGAGVRATPGILILPLEREF
                     HWSAATISAAVAVNIFLYGMVGPFAVAIIERFGLRRTVSLALVALAIGVGLTALMRAP
                     WQMMLLWGVLVGSGTGMVAMVLGATVAGRWFARHRGLVLGVLTASSATGQLVFLPLLA
                     AVALHFGWRWVSLTVAGALLILAPLAALLLRDRPSDIGLPRYGEDIVQPPVREGQNPA
                     RRALGALAQGARTRDFWLLSGSFFICGASTNGLVGTHLIAFCFDHGIPELTAASTLAL
                     MGFCDLIGTTASGWLTDRYDSKKLLAWYYGLRGLSLLFLPFAFDFTLYGLSLFAIFYG
                     LDWIATVPPTVRLAGKAFGEENAALMFGWIAASHQVGAAAAAWGAGIVRTETGDYFSA
                     FFFAGLLCLSAAMLVLFIGSGRKPAAFPIPLPAGA"
     gene            complement(2032..2526)
                     /locus_tag="VGM72_11590"
     CDS             complement(2032..2526)
                     /locus_tag="VGM72_11590"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY4265952.1"
                     /translation="MINRGNCPYIAHTSDFPPELCNCSALRQAARHVTKLYDDALAPL
                     NLGVNQYSILVRLHRLGPSALQALARALVTDRSTLGHLLRPLEARGLVRIETAKDDRR
                     GRTLRVTQAGLALIAQARPLWARAQRRFEGRIGTEAAAGLRGMLKRIAVEDFGEPKRA
                     VSEA"
     gene            complement(2700..3557)
                     /locus_tag="VGM72_11595"
     CDS             complement(2700..3557)
                     /locus_tag="VGM72_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265953.1"
                     /translation="MFAGLAAAIALTASSGSGQSKDSIPQFGGNGMAWAVNFWDYILP
                     PPAGSGHGPILTDPAYPYNSQIQNGRLFREGELRVSIVNAKDPILKPWAAKEMQATND
                     ELLSGKRDLGFVAQSRCWPGGVPGQLLFIEPVYFLQTPKEVLIVWQRNSTVRHIYMTD
                     HHSAHMKPSWFGESIGHYENGDTLVVDTIGIQGGKYHYIDNFRTPHTDKLHVVERFTI
                     SPDSKVLTAIVKVEDPDAFNAPLTLRETWRRNDVPIEEAVCADDGDAGVNLFKQKLEP
                     MPAAATADF"
     gene            complement(3613..4347)
                     /locus_tag="VGM72_11600"
     CDS             complement(3613..4347)
                     /locus_tag="VGM72_11600"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY4265954.1"
                     /translation="MIEKQVDVKTPDGVADCDLFYPGENGQWPAIIMYTDIGGRRPVF
                     VEMGKRLAAEGFVVLVPNPFYRLSRAPVYRDFKFGTEATTARMGEVRKFVTPAGIDSD
                     AHAYIAFLGGLKQVTGKMGSVGYCMGGGMAIRTAAAEPQKIAAAASFHGGNLATDAPD
                     SPHLLASKIKARLYFGFAIEDRSTPPEAVEKLKAALDAVGVNYEGETYPGARHGWCVK
                     DHNVYDEHQAEHAWHHLVELFKETLE"
     gene            complement(4503..5105)
                     /locus_tag="VGM72_11605"
     CDS             complement(4503..5105)
                     /locus_tag="VGM72_11605"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY4265955.1"
                     /translation="MTMKIYGARQSRASRVIWCANELGVPYEFIDIPWENQKNPDFLA
                     INPCGKFPGFVDGDLKLFESLAINLYVAKKYGTGELYPTAVEDEARTFQWTLWAAAEV
                     EPNAIPSLMVQIGRSKDTAGAAAAAEKLKPALKILNDCLKDRDYLLGKKFSIADLNVA
                     AVMSMTRGGKIDISYAPNVVAWLERCLARPARTAAPKVKE"
     gene            5420..>5844
                     /gene="atpH"
                     /locus_tag="VGM72_11610"
     CDS             5420..>5844
                     /gene="atpH"
                     /locus_tag="VGM72_11610"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEY4265956.1"
                     /translation="MASDNSHIAGLALRYANAVFELAAEERAIETVERDFASVKTMLK
                     DSPELTRLVRSPLFSREDQAKGMEAVLAKAEAGDLTRKLVLLLAQKRRLYVLGEIIGA
                     FENLLARHRGEIAAEITSARALSPDETAELRRVLKDKLGR"
BASE COUNT         1136 a         1824 c         1813 g         1071 t
ORIGIN      
        1 cggtgcgggg ctcgacgtgt tcgaacatga acccaaggtc catcccactc tgctggggct
       61 gacgaacgtc gcgctgctgc cgcatctggg ctcctcgacc atcgaggcgc gcaccgggat
      121 gggcaacaag gtgatcgcca atatcgcggc cctcgtggag ggaaagccgc tgccggacgg
      181 ggttagcccc ccggtctgac ctactccctc cccgattttt cgggacgagc cggcagattt
      241 ttcagcattt gcgtgaggcg caagggggag ctgcgcgata atctcccgtg gctgaaacgc
      301 aaagcgcgac aatcttccct tggaagccgg ggggccggtg ttctagaaac accgccatcc
      361 aaggagcgcc catgacccag acgatctacc gttccctcgc cgccgccgca ctcgcccttt
      421 ccggcgccgc cgcgctggta gccgccgcga tccccgcgct cgcgcagagc cttcccaacg
      481 gcaatggcaa ggagttggtg gccacgatct gcacgagttg ccacgatctt tctcccgtca
      541 ccgaaggcgg cttcagcaag gacgactgga acatggtcat caaaagcatg atcgagatgg
      601 gcgcggacat caaacccgat caggcgacgg ttctggtcaa ttacctcgcc accaatttcc
      661 cgccaaaacc gaaacaataa tcaccgggct atcctatggt gacggccggc gggcggtctc
      721 ggtcagtccg gagaccccgg ccgtattatg ctccggccgg gagggggatg gggaacgcgg
      781 ccggtttgcg gcccgagccg atgaacagta ccagcatcgc cgccgacaga cacagcaatc
      841 cggcaaagaa gaaggcgctg aaataatcgc ccgtctcggt gcgcacgatc cccgcacccc
      901 aggccgcggc cgccgcaccc acctgatggg aggccgcgat ccagccgaac atcagggccg
      961 cgttctcctc gccgaaggct ttgcccgcga ggcgcaccgt cggcggcacc gtggcaatcc
     1021 aatcgagccc atagaagatc gcgaacagcg acagtccata gagcgtgaaa tcgaacgcaa
     1081 acggcaggaa caagagcgac agaccgcgca agccgtaata ccaggcaagc agtttcttgc
     1141 tgtcgtagcg atcggtcagc catcccgacg cggtggtgcc gatgagatcg cagaatccca
     1201 tcagcgccag cgtgctcgcc gccgtcagct ccgggatgcc atgatcgaag cagaacgcga
     1261 tcagatgcgt gccgacgagc ccattggtgg atgcgccaca gatgaaaaaa cttcccgaga
     1321 gcagccagaa atcgcgcgtg cgggcgcctt gcgccagtgc gccaagcgcg cggcgcgccg
     1381 gattctggcc ctcgcgcacc ggcggctgaa cgatgtcctc accatagcgc ggcaagccga
     1441 tgtcggacgg gcggtcgcgc aagaggagcg cggccagagg cgccaggatc agcagggcgc
     1501 ccgccaccgt cagcgatacc caacgccagc cgaaatggag tgcgacggcc gcaagcagcg
     1561 gcaggaacac gagttggccg gtggcgctgc tcgcggtgag cacgcctagc accagcccgc
     1621 gatgacgcgc gaaccagcgt cccgcgacgg ttgcacccag caccatcgcg accatgccgg
     1681 tgccggagcc gaccagcacg ccccacagca gcatcatctg ccagggcgcg cgcatcagcg
     1741 cggtcaatcc cacaccgatg gcgagtgcca cgagcgcgag actgaccgta cggcgcaagc
     1801 cgaagcgttc gatgatggcc accgcgaacg gccccaccat gccgtagagg aagatgttga
     1861 ccgcgacggc ggcgctgatg gtcgcggcgg accagtggaa ttcgcgttcc agcggcagga
     1921 tgaggatgcc cggcgtcgcc cgcacgcctg caccgaccaa caggatcagg aaggtgatgg
     1981 cagcgaccgt ccagccataa tggaattttc gggtgcggga tacggacatg gtcaggcctc
     2041 gcttacagcg cgtttgggct cgccgaaatc ctctaccgcg atgcgtttga gcatgccgcg
     2101 cagcccggcg gcggcttccg taccgatccg cccctcgaag cgccgctgcg cgcgggccca
     2161 gagcggacgg gcttgcgcaa tcaaagcgag gccggcttgc gtcacccgta gcgttcggcc
     2221 acgccggtcg tctttggcgg tttcgatgcg caccagaccg cgcgcctcca gcgggcgcag
     2281 caaatgaccg agtgtggagc ggtccgtcac cagcgcgcgg gcgagcgcct gcagcgcgct
     2341 cgggcccagg cgatggagcc gcaccaggat ggaatattgg ttgactccga gattgagcgg
     2401 cgcgagcgcg tcgtcataga gctttgtcac atggcgtgcg gcctgacgca gcgcgctgca
     2461 attgcacagt tcaggcggga aatcgctcgt gtgcgcgata taggggcaat tgcccctatt
     2521 aatcaagacc cgtgacgagt gttctttttt accttcctct tgaggggaga cggttttgga
     2581 gggaagtggc ttggactatg tcgcgctcaa gagatcgtcg atggcgctgg ttgtcccgat
     2641 accatcgatc gtaacgtaga aatcattcgc caggatttgc gggccttttc gagtcgcgat
     2701 cagaaatccg ctgtggcggc tgcgggcatg ggctccaatt tctgcttgaa aagattcacg
     2761 ccggcatcac cgtcatcggc gcacaccgcc tcttcgatcg gtacatcgtt acggcgccaa
     2821 gtctccctca gggtgagggg cgcgttgaac gcgtcggggt cttcgacctt tacaatggcg
     2881 gtcaacacct tggaatccgg gctgatggtg aagcgctcga caacatgcag tttgtcggtg
     2941 tggggcgtgc ggaaattgtc gatgtagtga tatttgccgc cttggatgcc gatcgtgtcc
     3001 accaccagcg tatcgccatt ctcgtaatgg ccaatggatt cgccgaacca ggacggcttc
     3061 atatgggccg aatgatgatc ggtcatataa atgtggcgca ccgtgctgtt gcgctgccaa
     3121 acgatcagca cctccttcgg ggtctgcagg aaatagaccg gctcgataaa gagaagctgc
     3181 cccggaacgc cgccaggcca gcagcgcgat tgcgcgacaa agcccaaatc tcttttgccg
     3241 ctcaggagtt cgtcattggt cgcctgcatc tccttggccg cccagggctt caggatcggg
     3301 tccttcgcgt tgacgatgga gacccggagt tcgccttcgc ggaacagccg cccgttctgg
     3361 atctggctgt tatagggata ggcgggatcg gtgaggatcg ggccatgccc cgaacccgcc
     3421 ggcggcggca ggatgtaatc ccagaaattc acggcccagg ccattccatt gccgccaaac
     3481 tgcggaatcg aatcttttga ctgcccggag ccgctggatg cggtgagcgc gatcgcagcg
     3541 gcgaggccag cgaacattgg tcctctggcg atttttcctc tcatgtttga gtttcctcct
     3601 gggaagagcg tcctactcca gcgtctcctt gaacaactcc accagatgat gccacgcatg
     3661 ttcggcctga tgctcatcat agacattatg gtccttcacg caccagccat ggcgcgcgcc
     3721 gggataggtc tcgccctcat aattcacgcc taccgcatcg agtgctgctt tcaatttctc
     3781 caccgcttca ggcggcgtgg agcggtcttc gatggcgaag ccgaaataca gccgcgcctt
     3841 gatcttcgat gccaggagat ggggcgaatc cggcgcatcc gtggcgagat taccgccatg
     3901 gaaggaggcg gcggcggcaa tcttctgcgg ctcggcagcg gcggtgcgta tcgccatgcc
     3961 gccacccatg caatagccca cgctgcccat tttgcccgtc acctgcttca ggccgccgag
     4021 aaacgcgata taggcatgcg catcggaatc gatgcccgcc ggggtgacga atttgcgcac
     4081 ctcgcccatg cgcgcggtgg tggcctcggt gccgaatttg aaatcccggt acacgggcgc
     4141 gcgggaaagc cggtagaacg gattgggcac cagcactacg aaaccttcgg cggcaaggcg
     4201 ctttcccatt tcgacgaaca ccggccggcg gccgccgata tcggtgtaca tgatgatggc
     4261 gggccattgg ccgttctcgc ccggatagaa cagatcgcaa tccgcgacgc cgtccggggt
     4321 tttcacatcg acctgttttt caatcaccta actctccaat ctgacaggaa acgggataac
     4381 gcaaagaaca caaggcgagc acgaagggca caaagagctt ttcgtgttct tggtgccttg
     4441 tcttcgtggc ctttgtgtct ctcttctgtt tttcagcgct tttcgcgccg ttcgcataca
     4501 ttctattcct tgaccttggg tgcggcggtg cgggcggggc gcgccagaca gcgttccagc
     4561 cacgccacca cattgggggc gtaggaaata tcgatcttgc cgccgcgcgt catggacatc
     4621 acagccgcca cgttcaggtc tgcgatgctg aatttcttgc cgaggagata gtcgcggtcc
     4681 ttcagacaat cgttcagaat cttcagcgcc ggtttcagct tctcagcggc tgcggcggca
     4741 cccgccgtat ccttgctgcg gccgatctgg accatgagcg atgggatagc gttgggctcg
     4801 acttcggccg ccgcccacag cgtccattgg aaggtgcgcg cttcgtcttc gacagcggtg
     4861 gggtagagtt cgcctgtccc gtatttcttc gcgacgtaaa ggttgatcgc aagcgattca
     4921 aacagcttca gatcgccgtc cacgaaaccc gggaacttgc cacacgggtt gatggcgagg
     4981 aagtcgggat tcttctggtt ctcccaggga atatcgatga attcataggg gacgccgagt
     5041 tcgttcgcgc accagatcac gcgcgaggcg cgcgactgcc ttgcgccgta gatcttcatg
     5101 gtcatggttt cctccgccgg ctgattgagg cgggcaggca agaaccgcga caggtttttg
     5161 tcaagcgccg gaacggcttg ccacggggtt tcgcgacggc gcgagggaat taacccgcgt
     5221 ccgcgcaagc catgctatag cgcaagcgct tctcgcgggg gccgaagcgc cgcaccccat
     5281 caatgttgcg caggtttaat gcccatgcta agtcccggcg atgggcgtat ggggtcgctt
     5341 ctgttcgttt aattgcagtt aaaacaatgg cttagccgca ccgccccggc tgagccgcat
     5401 cctggtaagg caataaatct tggcctccga caacagccat atcgcgggtc tggcgctgcg
     5461 ctacgcgaac gcggtcttcg aactggccgc cgaagagcgc gccatcgaaa ccgtcgagcg
     5521 tgattttgcg tcggtcaaaa ccatgctgaa ggatagcccc gagctgacgc gcctggtgcg
     5581 ctcccccctg ttctcgcgcg aagaccaggc gaagggcatg gaagcggtgc tcgctaaagc
     5641 ggaggccggc gatctgaccc gcaagctggt gctgctgctg gcgcaaaagc gtcgtctcta
     5701 tgtgcttggc gaaatcatcg gcgccttcga aaatctgctc gcgcgccatc gcggcgaaat
     5761 cgccgccgaa atcacctcgg cgcgcgccct atcgccggac gaaaccgcgg agctgcgccg
     5821 cgtactgaag gacaagctcg gccg
//
LOCUS       DASYEG010000274         5620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4453247, whole genome shotgun sequence.
ACCESSION   DASYEG010000274 DASYEG010000000
VERSION     DASYEG010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4453247"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..325
                     /gene="rpmB"
                     /locus_tag="VGM72_11615"
     CDS             20..325
                     /gene="rpmB"
                     /locus_tag="VGM72_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011538016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEY4265957.1"
                     /translation="MARRCELTGKGVMSGHLVSHANNRKKRVFRPNLQTVSLASETLG
                     QNVSLKVSMNALRTVDRIGGLDIFLLKARDTALSTKALRLKRQIAKKQAKTEAASAA"
     gene            complement(322..1347)
                     /locus_tag="VGM72_11620"
     CDS             complement(322..1347)
                     /locus_tag="VGM72_11620"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HEY4265958.1"
                     /translation="MRTRYLSILAAGFALALSPLGLAAEQADEPATLQASRIPLYPGN
                     PDERRAGALIYRGGLVLRSSNAAFGGWSDLAVSADGAEILSISDEAHWLRAQLVYASN
                     GDLVGAGDAEIAPMLNMQGQPMHGKEGDAEGLTLERPNDLHGTVAVSFERNVRIWRYD
                     LSKGFGAKPTNVPIGDWVKPLHDNKQLEAITLFKPGTLMAFAEMKVNPGDDLLAAIEA
                     YPNPGGTPETRMLSVVPHDPFAITSVANAPDGGLYLLERRFSILGGVGMELRHIPASE
                     IHEGARMNGEVLVNLSFQDANIDNMEGLAVRRGPKGEILLYIISDDNYSPLQRTLLLM
                     FEVKDGA"
     gene            complement(1344..3197)
                     /gene="cobT"
                     /locus_tag="VGM72_11625"
     CDS             complement(1344..3197)
                     /gene="cobT"
                     /locus_tag="VGM72_11625"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HEY4265959.1"
                     /translation="MNPENPTDPFKRAVANAVRSMAGEPELEVTFSPEQPMLRGKKAR
                     LPLPSRSLPKDEVAAVRGAGDAYALKLAHHEDAIDRELKPAGTDAGAIFEAAEQARVE
                     AIGALAMPGVRDNLNAILTMRCKAHGLADVKEQIQAPLIDVLSLMMRERLTGDPPPEI
                     AKRAVDLWRPFVEAKVGSDLDKLRDNVRSQRSFGKITRAILTGLELADEYIDEPEGEE
                     ESPEDSNQDQSESDQESQGEDEEPSTLETEKKESSEAGEDVAGDMKAEDSDAQDSQET
                     KEGKRPFRPDLPFSDAERWDYKVFTSQFDEIMNAEDLCDAEELTRLRNFLDQQLAPVQ
                     GLVARLANKLQRLLMAQQNRSWDFDLEEGLLDTARLSRVVTDPMHPLSFKMEREIDFR
                     ATVVTLLLDNSGSMRGRPIMVAAMCADILARTLERCAVKVEVLGFTTRAWKGGQSRER
                     WLNEGKPTHPGRLNDLRHIVYKSADVPGRRTRKNLGLMMREGLLKENIDGEALIWAHS
                     RLIARPESRRILMVISDGAPVDDSTLSVNAGNYLDKHLRQVIAEIENKSSVELVAIGI
                     GHDVTRYYSRAVTILDAEQLAGAMTDQLIDLFAQDVKRGATGSRSRRHRAA"
     gene            complement(3404..4390)
                     /gene="cobS"
                     /locus_tag="VGM72_11630"
     CDS             complement(3404..4390)
                     /gene="cobS"
                     /locus_tag="VGM72_11630"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HEY4265960.1"
                     /translation="MPNNGLDAASAPDMTVDVRNTFGLDVAMSVPAFSKPNEYVPDLD
                     PAYVFDRETTLAILAGFAHNRRVLIQGYHGTGKSTHIEQVAARLNWPCVRVNLDSHIS
                     RIDLVGKDAIVLKDGKQVTEFREGILPWAIQHPVCICFDEYDAGRPDVMFVIQRVLEV
                     QGKLTLLDQNRVIRPHPSFRLFATANTVGLGDTSGLYHGTQQINQGQMDRWNIVVTLN
                     YLPHDTEVDIVLAKSQAYQTAEGKKTIAAMVRVADMTRNAFINGDISTVMSPRTVITW
                     AENATIFGDVGFAFRVTFLNKCDELERATVAEFYQRCFGEELPESAVKIMVA"
     gene            complement(4494..5117)
                     /locus_tag="VGM72_11635"
     CDS             complement(4494..5117)
                     /locus_tag="VGM72_11635"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY4265961.1"
                     /translation="MSSATYRPRFGYDIRINAERPQPRVTKPARRCASPGCRSAGEVN
                     VPKSRNNLDDRQWLCRDHLREHNARWDFFAGMSHEDIQRFCLDAITGHRPTWPLGKRA
                     AASADEKNGPNRATYASYHFEDGFAVFEEAAEPAEVQRRRLTKGQLDALAMLNLEENA
                     GLQEIKARYKELVKRFHPDANGGNRGTEERLRQVIKAYGHLRATGLA"
     gene            5204..5494
                     /locus_tag="VGM72_11640"
     CDS             5204..5494
                     /locus_tag="VGM72_11640"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEY4265962.1"
                     /translation="MTASRPVAEKLRDKFQAAFAPVELVIEDESSLHAGHSGTREGGE
                     SHFRVRIVSIAFAGLSRVERQRKVYTVAVAEIEAGLHALSLTTLTPDEAGRR"
BASE COUNT         1070 a         1679 c         1690 g         1181 t
ORIGIN      
        1 cgcaaaaggg atttggatca tggcacgccg ctgcgaattg accggaaaag gtgtcatgag
       61 tggacatctg gtgagccatg ccaacaatcg caagaagcgg gtattccggc caaatcttca
      121 gacggtgagc ttggccagcg aaactctcgg ccagaatgtc tcgctgaaag tcagcatgaa
      181 cgcgcttcgc acggttgacc gtatcggcgg gttggatatt ttccttctca aggcgcggga
      241 cacagcactt tcgacgaaag ccctccggtt gaagcgccag atcgcaaaaa aacaagcgaa
      301 gaccgaagcg gcatccgcag cttaggcgcc atcttttacc tcgaacatca atagcagcgt
      361 tcgctgcagc ggcgaataat tgtcgtccga aatgatgtac agaaggattt cgcccttcgg
      421 cccgcggcga acggcgagtc cttccatatt gtcgatgttg gcgtcctgga acgagagatt
      481 aaccagaacc tcgccattca ttcgcgcgcc ttcatggatt tcgctcgccg ggatgtggcg
      541 cagctccatg cccacacctc ccaggatcga gaatcggcgc tccaggagat aaaggccgcc
      601 atcgggcgca ttcgcgacac ttgtgatcgc gaatggatcg tgcggcacca cggacaacat
      661 ccgtgtctcg ggggttcctc ccggatttgg gtaggcctct atcgcggcca gaagatcgtc
      721 gcctgggttc accttcatct cggcaaaggc cataagcgta ccgggcttga agagcgtaat
      781 cgcctcaagt tgcttattgt catgcaaggg cttgacccaa tcgccgatgg ggacgttggt
      841 gggcttcgcg ccaaatcctt tgctcagatc gtagcgccag atgcggacat tgcgttcgaa
      901 gctgacagcg acagtcccgt gcaaatcgtt gggccgttcg agcgtgagcc cttcagcgtc
      961 gccctctttg ccgtgcatgg gctgcccctg catgttgagc atgggcgcga tttcggcgtc
     1021 gccagcccct acaagatcgc cgtttgaggc atagaccagc tgcgcccgca gccaatgcgc
     1081 ctcatcggag atcgagagga tttcggcgcc gtcggcgctg actgccaaat ccgaccatcc
     1141 accaaacgca gcgttggagg agcgaaggac aaggccgccg cgatagatca gcgccccggc
     1201 acgccgctcg tcgggattgc cgggataaag cgggatgcgc gaggcctgca gcgtcgcggg
     1261 ttcgtccgcc tgttccgccg caagcccaag cgggctgagc gcaagggcga acccagcggc
     1321 cagaatagag aggtatcgag ttctcacgcg gccctatggc ggcgcgaacg cgagccggtg
     1381 gcgccgcgtt tcacatcctg cgcgaacaga tcgatcaact ggtccgtcat ggcgcccgcc
     1441 aattgctcgg catccagaat ggtgaccgca cggctataat agcgcgtcac gtcgtggccg
     1501 atgccgatgg cgacaagctc cacgctcgac ttgttttcaa tttcggcgat cacctgccgg
     1561 aggtgcttgt cgagataatt tccagcattc accgacaatg tcgaatcgtc caccggcgcg
     1621 ccatcggaaa tcaccatcag gatgcggcgg gattcaggcc gtgcgatcag ccggctatgg
     1681 gcccagatca gtgcctcgcc gtcgatgttc tccttgagaa gaccctcgcg catcatgagg
     1741 ccaagattct tgcgtgtgcg ccggcccggc acatcggcag acttgtagac gatgtggcgc
     1801 agatcgttca acctgccggg atgcgtgggt ttgccttcgt tcagccagcg ctcacgcgat
     1861 tgaccgccct tccaggcgcg tgtggtgaag ccgagcacct ccactttcac ggcgcatcgt
     1921 tcgagcgtgc gtgcgaggat gtccgcgcac atcgcggcca ccatgatggg gcgcccgcgc
     1981 atcgaaccgg aattgtcgag cagcagcgtc accaccgtag cgcggaaatc gatctcgcgc
     2041 tccatcttga aggagagcgg gtgcatcgga tcggtgacga cgcgggacaa tcgcgcggtg
     2101 tccagcaatc cctcttccaa gtcgaagtcc caggagcggt tctgttgtgc catcagcagg
     2161 cgctgcagct tgttcgccag ccgggcgaca aggccctgca cgggagcaag ctgctggtcg
     2221 aggaagttgc gcaaacgtgt gagctcttcc gcgtcgcaca aatcttccgc gttcatgatt
     2281 tcgtcgaact ggctcgtaaa tactttgtag tcccagcgtt cggcatcgga gaatggaagg
     2341 tccggacgga acggacgctt gccctctttc gtctcctgag agtcctgcgc gtcagaatct
     2401 tcggctttca tatcgcccgc gacatcctcg cccgcttcgc tcgattcttt cttttcggtt
     2461 tcgagcgtag agggttcttc gtcctcgcct tgcgattctt gatcgctttc actctggtcc
     2521 tggttcgaat cttcgggcga ttcttcttcg ccttccggtt cgtcgatata ctcatcggcc
     2581 agctcgagcc cggtcaggat tgcgcgcgtg atcttcccga aactacgctg actgcgcaca
     2641 ttgtcccgga gtttatcgag atcggatcca accttggcct cgacgaatgg gcgccacaga
     2701 tcgactgcgc gcttggcgat ctcggggggc ggatcaccgg tcagccgttc gcgcatcatg
     2761 aggctcagca cgtcgattag aggagcctgg atctgctcct tcacgtcggc gaggccatgg
     2821 gccttgcagc gcatggtgag gatggcgttg agattgtcac gcacgcccgg catggcgagt
     2881 gcgccgatgg cctccacccg cgcctgttcg gcggcctcga agatggcccc agcatcggtt
     2941 cccgcgggct tgagctcgcg gtctatggcg tcctcgtgat gggcgagctt cagcgcatag
     3001 gcatccccgg cgccgcgcac ggcggcaact tcatccttgg gcagcgagcg cgagggcagg
     3061 ggcagccgtg ccttcttgcc gcgcagcatc ggctgttcgg gcgagaaggt gacctcgagt
     3121 tcgggttcgc ccgccatcga gcgcacggcg ttcgccactg cgcgcttgaa tgggtcggta
     3181 ggattttcgg ggttcatcgg cacgggactt actcagcaag accagtttga ttggatgtgg
     3241 cagcgatcct acagcttgtg cgtcaaaacc ttacaacccc ctatgaattg ttttgccgga
     3301 aaaaatgggg ggtggggccc ttccttctta tgcgtttggg cttatgcgtt tagggaaccg
     3361 tcccaaaaac tatagagcgt caccgggcac tgcaaaagga cctctatgcg accatgattt
     3421 tgacggcgga ttccggaagc tcctcaccga agcagcgctg ataaaactcg gccacggtcg
     3481 cgcgttccaa ctcgtcgcat ttgttcagga aggtgacgcg gaaggcgaag ccgacatcgc
     3541 cgaagatggt cgcgttctcc gcccaggtga tgaccgtgcg cggactcatc acggtcgaga
     3601 tgtcgccatt gatgaaggcg ttgcgcgtca tgtcggcgac gcgcaccatc gcggcgatgg
     3661 tctttttgcc ttccgccgtc tgataggcct gcgatttcgc gagcacgatg tcgacttcgg
     3721 tatcgtgcgg cagataattc agcgtcacca cgatgttcca gcgatccatc tgtccctgat
     3781 tgatctgctg cgtcccgtga tacagtcccg acgtgtcgcc gaggccgacc gtgtttgcgg
     3841 tcgcaaacag ccggaacgag gggtgaggcc gaatcacgcg attctgatcg agcagcgtaa
     3901 gcttgccctg cacctcaagc acgcgctgga tcacgaacat cacgtccgga cgccccgcat
     3961 cgtattcgtc gaaacatatg cacaccggat gctgaatcgc ccacggaagg attccttcgc
     4021 ggaactcggt cacctgtttg ccgtccttca acacgatcgc gtccttgccg accagatcga
     4081 tgcggctgat atgggaatcg aggttcacac gcacgcaggg ccaattcagc cgggccgcga
     4141 cctgttcgat atgggtcgac ttcccggtcc catgataacc ctggatgagc acgcggcggt
     4201 tgtgcgcaaa gccggccagg atagccaacg tcgtctcgcg gtcgaagaca taggccggat
     4261 ccaggtcggg cacatattcg ttcggcttcg agaaagccgg tacgctcatc gccacgtcca
     4321 atccgaacgt gttgcggacg tcgaccgtca tatccggggc ggaggcggca tcaagaccat
     4381 tgttcggcat tcttcaatcc atctgtcatg gggcgttaga gaggaaatta agggcgagct
     4441 gccaatgctg cgtcccctgg gcctgaatcg tcccaggagc gttcctcgcc ggcttatgcg
     4501 agacccgtcg ccctgagatg tccgtaggcc ttgatgacct gcctcagccg ctcttcggtg
     4561 ccccggtttc cgccgttggc gtcggggtga aagcgtttca caagttcctt ataccgcgcc
     4621 tttatctcct gcaagccggc gttttcttca aggttcagca tggcgagcgc gtccaattgt
     4681 cccttggtca atcgccgccg ctggacttcg gcgggctctg cggcctcctc gaacaccgca
     4741 aacccatcct cgaaatgata gctcgcatag gtggcacggt tggggccgtt tttctcatcg
     4801 gcactggctg ccgcgcgctt gcccagaggc caggtcggcc ggtgtccggt tatggcatcg
     4861 aggcagaagc gctgaatgtc ctcatggctc atgccggcga aaaaatccca gcgtgcgttg
     4921 tgctcacgca ggtggtcgcg gcaaagccac tgccggtcgt cgagattgtt gcgcgatttc
     4981 ggcacgttga cctcgccggc cgaacggcac cctggcgatg cgcatcgtct tgccggtttg
     5041 gtgacgcgcg gctgtggccg ctcggcattg atgcggatgt cataaccaaa acgcgggcgg
     5101 taagttgccg aactcatggt cccattatgc ggtgccagtc gtaaaagaca agaaaacgaa
     5161 acccttgaca ggcatggccc gctcacatct agagcattgt attatgaccg catcgagacc
     5221 tgtcgccgaa aaattgcgtg ataaattcca ggctgccttt gcgccggttg aacttgtcat
     5281 agaagatgag agttctcttc atgccgggca ttccggcact cgtgaaggag gagaatcgca
     5341 ttttcgcgtg cggatcgttt cgatcgcatt cgcggggctt tctcgcgttg agcggcaacg
     5401 caaggtttat acggtggcgg tcgccgaaat cgaggccgga ttgcacgcgc tgtcccttac
     5461 gacattgact ccggacgaag caggacggcg ttaaccgctg tcgtcgtccg gctcgcgcag
     5521 aggtggggtg atgcgcagag ccatgatctg gttgcgctgc cgccgcagaa tttcgaattt
     5581 gaatccgaag aacgaaaaca cctggcccac ctccgggatc
//
LOCUS       DASYEG010000275         5335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1990603, whole genome shotgun sequence.
ACCESSION   DASYEG010000275 DASYEG010000000
VERSION     DASYEG010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1990603"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VGM72_11645"
     CDS             <1..747
                     /locus_tag="VGM72_11645"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4265963.1"
                     /translation="QGVYARIKLLDHLSKTYSCILFDRRECGQSGGRVERVTWAHYVA
                     QGKGLLDHLGIVRAHIMGGCMGCCPVAAFGTAHPEATLSMILFWPVGGAKYRLTCHQR
                     FAVHLAYVQGHGLERVVELVAASGKSFGEDPRGGPWASVIRQNRGFADSYARQDVEKY
                     RLIVAGMGRTLFDRDTAPGAEPEDLLRLGIPTLIVPGRDASHATSAARYLEECIPNAE
                     YWDMLPAGQTEDNAPTRLLEFLDKAATAPA"
     gene            complement(744..1325)
                     /locus_tag="VGM72_11650"
     CDS             complement(744..1325)
                     /locus_tag="VGM72_11650"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEY4265964.1"
                     /translation="MNRRAVIALGLLFAGSAFAGASYAATDKYVTILDGKSMGDFTPI
                     GNANWRIKDGAVYADRGNGFLVSKQNYADFHLRAEVWVDQPANSGIFIRCENPQNVSG
                     RSSYEVNIFDTRPDQSYGTGSIVNVAKVSPMPHAAGKWNVMEITAKGPLMVITFNGKV
                     SSSGSDAKHPRGRIALQYGGGVVKFRKVEIMPL"
     gene            complement(1476..1964)
                     /locus_tag="VGM72_11655"
     CDS             complement(1476..1964)
                     /locus_tag="VGM72_11655"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY4265965.1"
                     /translation="MKKFVLATGLAFAGMVAVPANAQLVPHKDMSVTTAVTIAQTAVQ
                     TCKSQGYNVSAHVLGRSGEVLVAMRGDDTGPQTYENSFKKAWTARAQRAPSLNFANAV
                     KANPTAGAIHLSNMVPAQGGLPIMVGDDAIGAVGISGAPGGDKDEACAKAGIDKVAAE
                     LK"
     gene            complement(2054..2548)
                     /locus_tag="VGM72_11660"
     CDS             complement(2054..2548)
                     /locus_tag="VGM72_11660"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY4265966.1"
                     /translation="MLKTLTGISLGVAVVCACAAPAGAQLLTHKDLSVATATTIAMTA
                     IETCKAQGYNVSANVLGREGQVVVAIRNPAAGFGTYENSMKKAFTARATRAPSGKFAQ
                     TVKGNPNAPQFFLSNYVAAQGALPIMVGNDVIGAVGISGAPGGDKDEACAKAALDKVA
                     AQLR"
     gene            2833..3459
                     /locus_tag="VGM72_11665"
     CDS             2833..3459
                     /locus_tag="VGM72_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265967.1"
                     /translation="MQAVAAQFLPILSPVSWVPLLGAFSDSVRSHFPKGMLAMAGLRA
                     RMLAAAVICAVTAITSAIAQQATAGSSANPGLRSPRPAEIIQRDPPSSSGAPYIQAVT
                     LRGERAGGHLYIVQEIAYRAAKGNATRLRFQLVSVSKTPLKVTVRDHAIRSASARQQR
                     GTFIAVRFRCGPFEKNYSHVTRATILDADGERSNSVDFTVSCNLATIS"
     gene            complement(3504..4436)
                     /locus_tag="VGM72_11670"
     CDS             complement(3504..4436)
                     /locus_tag="VGM72_11670"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4265968.1"
                     /translation="MLKFKTVTRTQGNNQALKDGSVKPKTFEFDFVEVNPLIDAFRRM
                     VRGLEFDICEMAITTYICARANGKRMTAVPVFLVRAFHHGAILVNTNAGIRTPKDLEG
                     RRVGVNRGYTVTTGVWARGILQEEYGVDLSKVTWVLSGDEHVAEYVPPKNVVPVEKGK
                     KIADMLIAGELAAAIGVEVDHPDVKPLIPNALEAGLASLKRSGHYPINHTIVIKDELI
                     AAHPGLAADVFNAFAQSKRLYLERLKNGAIEKPTEADKVHARVMDIIGDPLPYGVGAN
                     RRVIEELIGHAVTQGIIAKPVTVEELFAPETLAL"
     gene            complement(4488..>5335)
                     /locus_tag="VGM72_11675"
     CDS             complement(4488..>5335)
                     /locus_tag="VGM72_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265969.1"
                     /translation="GLAAMFLCVADARAAAPIPDFSGIWGRNSLDYEPPASGPGPVMN
                     TTHSFFKRMGDYTNPILKPESAARVKAGGVLSAKGENFPTPSSLCWPMSPPFIWRIPG
                     VQLLQETGKVTILYLNDHHVRHIRLNAAHPARVVPSWTGDSVGHYEGGTLVVDTVGIK
                     VGPYSMTDNYNSPQSAALHLVERFRLIDGEAAKKAAEQSERDSGRVDADRGGLSIDPT
                     YKGRGLEVELRIEDPNVFTMPWTARVTYRRVQDAWEERVCADNPHDYSTGADTPVPKA
                     ATPDF"
BASE COUNT         1010 a         1654 c         1591 g         1080 t
ORIGIN      
        1 caaggcgttt atgccaggat caaattgctc gatcatctgt cgaagacata tagctgcatt
       61 cttttcgacc ggcgcgaatg cggccaatcg ggagggcggg tggagcgcgt gacatgggcg
      121 cattacgtgg cgcaaggcaa aggcctgctc gatcacctcg gcatcgtccg cgcgcacatc
      181 atgggcggtt gcatgggctg ttgtcccgtg gcggcattcg gtaccgcaca ccccgaagcg
      241 acgctgagca tgatcctttt ttggccggtt ggcggcgcga aataccggct cacctgtcac
      301 cagcgctttg cggtgcatct ggcctatgtg cagggccacg gcctcgaacg cgtggtcgaa
      361 ctcgttgcgg catccggtaa aagcttcggt gaagacccgc gcggcgggcc gtgggcgtcc
      421 gtcatccggc agaaccgtgg ctttgccgat tcctacgcgc ggcaggatgt cgagaaatat
      481 aggctgatcg tcgccggcat gggccgcacc ctgttcgacc gcgacacagc accgggagcg
      541 gagcccgagg atctgctgcg cctcggcatc ccgacgctga tcgtgcccgg acgcgacgca
      601 agccatgcca catcggcggc gcgctatctc gaagaatgca ttcccaacgc cgaatattgg
      661 gacatgcttc cggcaggcca gacggaggac aatgccccca cccgcttgct ggagtttctc
      721 gacaaggcgg cgactgcgcc tgcttagagc ggcataatct ccaccttacg gaatttcacg
      781 acgccgccac catattgcag cgcgatccgg ccgcgcggat gcttcgcatc cgatccgctt
      841 gaggaaactt ttccgttgaa ggtgatgacc atcagagggc ccttggccgt gatttccatg
      901 acattccatt tgccggcggc gtggggcatc ggcgacacct tggcgacgtt gacgatggaa
      961 cccgtgccgt aactctggtc ggggcgcgtg tcgaagatgt tcacctcata agaggatcgg
     1021 ccactgacat tttgaggatt ctcgcaacgg ataaaaatgc ccgaattcgc cggctgatcc
     1081 acccagacct cggcccgaag atgaaaatcg gcataatttt gctttgagac gagaaaccca
     1141 ttgccgcggt ctgcgtacac cgcgccgtcc ttgatgcgcc aattggcatt accgatgggc
     1201 gtgaaatctc ccatgctctt gccgtcgaga atcgtgacgt atttgtccgt cgcggcgtat
     1261 gacgcgccgg caaatgcgga cccggcaaac aaaagtccca aggcaatgac ggccctgcgg
     1321 ttcatgatgg tttccctctt cgtggcgtaa gcgtcacaac gacattatca gacaacgaca
     1381 ttatcagatt gacgggggag cagcgagagc accgcccctc acgaaaaacg gcgcaacccc
     1441 caaaagccgc gccgcttctt cgcgaagttg ctcgtctact tcagttcggc tgcaaccttg
     1501 tcgattccgg ctttggcgca ggcttcatcc ttgtcgccgc cgggagcgcc cgaaatgccg
     1561 accgcaccga tcgcgtcgtc gccaaccatg atgggcagcc cgccttgcgc cggaaccatg
     1621 ttgctcagat ggatcgcgcc ggcggtggga ttggccttca cggcgttggc gaaattgagc
     1681 gatggagcgc gctgggcgcg cgccgtccag gccttcttga acgagttctc ataggtctgc
     1741 gggcccgtat cgtcaccgcg catggcaacc agcacttcgc cgctgcgccc cagcacatga
     1801 gccgaaacat tgtacccttg cgacttgcag gtctgaaccg cggtctgcgc gatcgtcacc
     1861 gcggtcgtga ccgacatatc cttgtgaggg acaagctggg cattggccgg gacggcaacc
     1921 attccggcga aggcgagccc ggtggcaaga acgaatttct tcatagtgaa tctccttgaa
     1981 tgaaggccgc gtgagaacgc ggtttcgaga atggcgcgag gcgagcgatg cgcccggcct
     2041 ctgtccgatg ggcctacctc agttgagccg cgaccttgtc gagagcggct ttggcgcagg
     2101 cttcgtcctt gtcgccgcct ggcgcgccgg aaataccgac cgcgccgatg acgtcattgc
     2161 cgaccatgat cggcagcgcg ccctgcgccg ccacatagtt ggagaggaaa aattgcggcg
     2221 cgttcgggtt tcccttcacg gtttgcgcga acttgcccga tggcgcgcgc gtcgcacggg
     2281 ctgtaaaggc ctttttcatc gagttctcgt aagtgccgaa gccggccgca ggattgcgga
     2341 tggccaccac tacctggccc tcacgcccga gcacattggc cgacacattg tagccctgcg
     2401 ccttgcaggt ctcgatggcc gtcatcgcga tcgtcgttgc ggtggcgacc gacaaatcct
     2461 tgtgggtcaa aagctgcgcg ccggcaggtg ctgcgcatgc gcacaccacc gcaacgccaa
     2521 gcgaaattcc ggtaagcgtc ttcaacatag gattccctcc aggatcaaac cgcggttgcg
     2581 cggcataagt gttcacagct tagaacagtt ttgaaccgat ttggcgcggg aattttcctc
     2641 atcggatttg gccgtttcgc gaggacaatt cgacgtaggc cgcggttcgc cctctatact
     2701 tagtgcaaaa cgccctctcg cccccaatga attgttttgc cggaaaattg aggcgggggg
     2761 atgggggcgg tcctccccag gtagcacttt tccgtcaact cacggtgatg gatctaagca
     2821 ggattggctt gggtgcaagc ggttgcggcg cagttcctcc cgatactctc tccagtatca
     2881 tgggtgccgc tcctcggcgc attttctgat agtgtgagat cgcattttcc gaaaggaatg
     2941 ttggctatgg ccggtttgcg cgcgagaatg cttgcagccg cggtcatctg cgctgtcacg
     3001 gccatcacca gcgctatcgc gcagcaagcg acggccggct cttcggcaaa ccctggattg
     3061 cggtctccca gacccgcgga aatcatccag cgcgaccctc cctcctcgtc cggcgccccc
     3121 tacatccaag cggtcacgct gcgcggggag cgcgccggtg gacatcttta tatcgtccag
     3181 gaaatcgcct accgcgcggc caagggaaat gcgacacgtt tgcgctttca acttgtcagc
     3241 gtttcaaaga cgcccttgaa agtcaccgtg cgcgaccatg cgatccgcag cgccagcgca
     3301 cggcaacaac gcggaacctt tatcgccgtc cgcttccgct gcggaccgtt tgagaagaat
     3361 tattctcacg tgacacgcgc cacaatcctc gatgccgacg gcgagcgcag caactcggtc
     3421 gatttcacgg tctcttgcaa tttggcgacg atctcatagg ccccaggcgc cgcacagcgg
     3481 tgcccacgcg aacgaaatca tcctcacaag gcgagcgttt cgggcgcgaa caattcttcc
     3541 accgtcaccg gcttggcgat gatcccttgg gtcacggcat ggccgatcag ctcctcgatc
     3601 acgcggcggt tcgcaccgac accatagggc agcggatcgc cgatgatgtc catcacacgg
     3661 gcatggacct tgtcggcctc agtgggcttc tcgatggcgc cattcttgag ccgttccaga
     3721 taaagccgct tggattgcgc aaatgcgttg aagacatcgg ccgcaagacc gggatgggcc
     3781 gcgatcagct catccttgat cactattgtg tggttgatcg gataatggcc gcttcgcttc
     3841 agcgacgcga ggccggcttc cagcgcattc gggatcagcg gcttcacgtc gggatgatcc
     3901 acctccaccc cgatggccgc cgccaactcg ccggcgatga gcatgtccgc gatcttcttt
     3961 cccttttcga ccggcacgac gtttttcggc ggcacatatt cggccacatg ctcgtcgccc
     4021 gacagcaccc aagtgacctt tgaaaggtcg acgccatatt cctcctgcaa gatgccgcgc
     4081 gcccatacgc cggtcgtcac cgtgtagccg cgattgaccc cgacgcgcct gccttccaga
     4141 tctttcggcg tgcggatgcc ggcattggtg ttcaccagga tcgcgccatg gtggaaagcg
     4201 cgcaccagga agaccggcac cgccgtcatg cgcttgccgt tggcgcgggc gcagatatag
     4261 gtcgtgatcg ccatttcgca gatgtcgaat tcgagaccgc gcaccatgcg gcggaaggca
     4321 tcgatcagcg gattgacctc cacgaaatcg aattcgaagg tttttggttt gacgcttccg
     4381 tccttgagcg cctgattgtt gccttgtgtg cgggtgacgg ttttgaattt cagcatcgaa
     4441 ataatccctg gcgtgatgat ggaacggatg atgggcctcg cgcgtgctca gaaatcgggc
     4501 gtcgcggctt tgggcaccgg cgtatccgcg ccggtggagt aatcatgtgg attgtcggcg
     4561 cagacgcgct cctcccatgc gtcctgcaca cggcgatagg tcacccgcgc cgtccacggc
     4621 atggtgaaga cattcgggtc ttcgatcctg agttcgacct ccaatcccct gcccttatag
     4681 gtcggatcga tcgacagccc gcctctgtcc gcatccacac ggccgctgtc gcgctcgctt
     4741 tgctcggcgg ctttcttcgc ggcctcgccg tcgatcagcc ggaaacgttc taccagatgc
     4801 agcgcggcgc tctgcggcga gttgtaattg tccgtcatcg aataggggcc gaccttgatg
     4861 ccgacggtat ccacgaccag cgtgccgcct tcgtaatggc cgacggaatc ccccgtccac
     4921 gacggaacca cgcgcgcggg atgggccgca ttcaagcgaa tatggcggac atgatgatcg
     4981 ttcaaataga ggatggtgac tttgccggtt tcctgcagca attgaacgcc gggaatccgc
     5041 cagatgaaag gcggagacat cggccagcac aggctggaag gtgtgggaaa gttctcgcct
     5101 ttggccgaca gcacaccgcc cgccttcacg cgcgccgcgc tttcgggctt caggatcgga
     5161 ttggtgtaat cgcccatccg tttgaaaaac gaatgtgtcg tgttcatcac cggaccggga
     5221 ccggacgcgg gcggttcgta atccagcgaa ttgcggcccc agatgccgga gaaatccggg
     5281 atgggtgccg ctgcccgtgc atccgcgacg caaaggaaca tcgcggcgag gccga
//
LOCUS       DASYEG010000276         6110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1991095, whole genome shotgun sequence.
ACCESSION   DASYEG010000276 DASYEG010000000
VERSION     DASYEG010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1991095"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VGM72_11680"
     CDS             <1..703
                     /locus_tag="VGM72_11680"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEY4265970.1"
                     /translation="AAVGHMLAGLNGTIDMIVPRGGKSLVARVQAEARVPVLAHLEGI
                     CHTYVHEKADLAMARAIVLNAKMRRTGVCGATETLLIDRSILGTHLKPILDDLVKAGC
                     EIRGDAGIRAAYPSAQLANEDDWRTEYLDAVIAVKAVGGVEEAIAHIAKYGSQHTEAI
                     ITDDSGTAERFLSVLDSAIVIHNASTQFADGGEFGMGAEIGIGTGKLHARGPVGVEQL
                     TIFKYAVRGSGQIRP"
     gene            711..1349
                     /locus_tag="VGM72_11685"
     CDS             711..1349
                     /locus_tag="VGM72_11685"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEY4265971.1"
                     /translation="MSQARPTAHWVRPPGPVGHLRIGLLGGSFNPAHGGHIYASALAL
                     KRLRLDYVWWLVAPQNPLKDTHGMAAFDARLSGARAVAQHPRIVVTGIESALGTRFTI
                     DTLRALKRRFPGVHFVWLMGSDNLLQLPRWRDWQAIFASVPIAVVARPGSEVAARASK
                     AAVRFRTSYVAPDRHFSVMPAPAWTILDSRRNPVSATGLRTGATLAKNAPLW"
     gene            1540..1896
                     /gene="rsfS"
                     /locus_tag="VGM72_11690"
     CDS             1540..1896
                     /gene="rsfS"
                     /locus_tag="VGM72_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019977573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEY4265972.1"
                     /translation="MLARIQATLDDDKAEDLVTIDLTGRSPLTDAIVVATGRSARHVA
                     SLAENLARRLKEWGYGKVHLEGLPQGDWVLVDAGDVVVHIFRAEVRAYYDLEGMWSVD
                     ETSHRRRLSEHADSVA"
     gene            1844..2392
                     /gene="rlmH"
                     /locus_tag="VGM72_11695"
     CDS             1844..2392
                     /gene="rlmH"
                     /locus_tag="VGM72_11695"
                     /inference="COORDINATES: protein motif:HMM:NF000989.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEY4265973.1"
                     /translation="MKRAIGAASPNTPILSPEPMRLIVVAVGRARTAPEQTLCDLQWE
                     RATALGRKLGFTKLDFAIVETSHAGDVLARMEEEAKRLTSRVPAGARRIALDEAGRSL
                     SSAAFAKHLAQLRDRGERDLAFLIGGPDGLAPELRENAEERLAFGCQTWPHLLVRAML
                     AEQIYRAFAILSGHPYHRGRAN"
     gene            2450..2884
                     /locus_tag="VGM72_11700"
     CDS             2450..2884
                     /locus_tag="VGM72_11700"
                     /inference="COORDINATES: protein motif:HMM:NF022227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliX"
                     /protein_id="HEY4265974.1"
                     /translation="MEIKSTRKLESASPARRAGRAASLGGNSFSTEEIADAKPATALT
                     GTGPIAAVDTILALQGIEDSTSSRKRSVSHGEQLLTLLDELKDGLLAGGIPRAVLNRL
                     ALAISKRRESFVDPALQGVLDEIDLRARVELAKLEQAERPAV"
     gene            2980..3396
                     /gene="dksA"
                     /locus_tag="VGM72_11705"
     CDS             2980..3396
                     /gene="dksA"
                     /locus_tag="VGM72_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEY4265975.1"
                     /translation="MATDLPHGYRPSDEESFMNERQREYFRRKLVAWKDDILRESRGT
                     IQNLQSEPTPNADLADRATTEAERQLELRTRDRQRKLIAKIDSALRRLEDGSYGFCEE
                     TGEPISLKRLDARPIATLSVEAQERHERRERVYRDD"
     gene            complement(3460..4221)
                     /gene="flgH"
                     /locus_tag="VGM72_11710"
     CDS             complement(3460..4221)
                     /gene="flgH"
                     /locus_tag="VGM72_11710"
                     /inference="COORDINATES: protein motif:HMM:NF001305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEY4265976.1"
                     /translation="MIHILPRRALASAACTASLLLTSCASTVDKIEQIGKPPALSPIH
                     VQPNIVAGAPVQVPQPAIENLPPQANSLWQAGSRSFFRDPRAAKIGDLLTVQIAIGDQ
                     AKIANTTSRSTTASEDASAPNLLGLESRLKGILPKSVDPSSLVKLGSDSANTGTGTVD
                     RSETINLTVAAIVTQVLPNGNLVIQGQQEVRVNNEVRELSVSGVVRPEDISNTNTISH
                     TQIAEARISYGGRGQITDVQQPRYGQQFFDIVWPF"
     gene            complement(4238..4738)
                     /gene="flgA"
                     /locus_tag="VGM72_11715"
     CDS             complement(4238..4738)
                     /gene="flgA"
                     /locus_tag="VGM72_11715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HEY4265977.1"
                     /translation="MTLRFVLALAFCTVAFSAYAQDADNQTELVVPAHDIARGAVIAD
                     GDLTTKSFPVLRMNESFIHNLSDIEGKEAKRALRAGEVLRLSDLKRPALVAKGATVTM
                     VFDAPGMSLTAVGRALAEGGEGDSIAVLNPTSYRQVVAVVTAPGVVRVGPADASAKTN
                     EVAIAK"
     gene            complement(4744..5529)
                     /gene="flgG"
                     /locus_tag="VGM72_11720"
     CDS             complement(4744..5529)
                     /gene="flgG"
                     /locus_tag="VGM72_11720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEY4265978.1"
                     /translation="MRALSIAATGMLAQQNNVEVIANNLANMNTTAYKMQRAEFQDLL
                     YQNVERPGAASADTGAVLPAGIQIGVGVRTAATYRITAQGNLNSTSNPYDLAINGKGY
                     FHVQMPDGTDAYTRAGAFSLSPEGQLVTDKGYVVAPGVAIPQDATSVTINAQGQVQAT
                     IAGQNAPQTLGQLELARFPNEGGLQATGDNLYTETPASGAVLTGLPGSPGYGTLQQGY
                     LETSNVNAVQEITDLITAQRAYEMNSKVISAADQMLQITSKMS"
     gene            complement(5543..>6110)
                     /locus_tag="VGM72_11725"
     CDS             complement(5543..>6110)
                     /locus_tag="VGM72_11725"
                     /inference="COORDINATES: protein motif:HMM:NF018168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HEY4265979.1"
                     /translation="VAFVLDRGVARDLSEGRFDPTGAPLDLAIAGNGYFVVQTPDGER
                     YTRNGHFSLNDQGQVVTDDGYPVQSDGGAITLQPQDGDLKVGSDGTLSTDLQLLGKLR
                     VVEFANERALKKTGASNYDAGGQAAQASTTARVRQGMMEKSNVEPMIEISRMIEIMRA
                     YQTSADLTKSGEDLLKQAIEKLGAVPAS"
BASE COUNT         1118 a         1873 c         1905 g         1214 t
ORIGIN      
        1 cgcggcggta ggccacatgc ttgccgggct gaacgggacc atcgacatga tcgtgccgcg
       61 tggcggcaag agcctggttg cccgcgtgca ggccgaagcg cgcgtgccgg tgctggccca
      121 ccttgaaggg atctgccaca cctatgtgca cgaaaaagcc gacctcgcga tggcgcgcgc
      181 gatcgtgctg aacgcaaaga tgcgtcgcac cggcgtgtgc ggcgcgacgg aaactttgct
      241 gatcgaccgc agcattctgg gcacgcacct caaacccatt ctggacgatc tggtgaaagc
      301 gggatgcgaa atccgcggcg atgccggcat tcgcgcggcc tatccttccg cccagctcgc
      361 gaacgaagac gattggcgta cggaatatct cgatgccgtg attgcggtta aggccgtcgg
      421 cggcgtggaa gaagccatag cccatatcgc gaaatatggc tctcagcata ccgaagccat
      481 tatcacggac gattcgggaa cggcggagcg ttttctttcc gtgctcgatt cggccattgt
      541 cattcataat gcctcgacgc agttcgccga tggcggcgag ttcggcatgg gcgcggagat
      601 cggcatcggc accggaaagc tgcatgcgcg cgggccggtc ggcgtcgaac agctcacgat
      661 cttcaaatat gccgttcgcg gcagcggcca gatccggccg tgatccgcta ttgagccaag
      721 cccgtccaac ggcacattgg gtgcggccgc ccgggccggt gggtcatttg cgcatcggcc
      781 ttctcggcgg atcgttcaac cctgcccacg gcggtcacat ttacgcgagt gctctcgcgc
      841 tcaaacggct tcggctcgac tatgtctggt ggctggtcgc tccacaaaat cctctgaaag
      901 acacgcacgg catggctgcg ttcgacgccc gcctgagtgg cgcccgggcc gttgcgcaac
      961 atccacgcat cgtcgtgacc gggatcgaat ccgcgcttgg cacgcgattt accatagaca
     1021 cgctacgcgc attgaagcgg cgttttccgg gcgttcattt cgtttggctc atgggaagcg
     1081 ataatctgtt gcagctaccc cgctggcggg attggcaggc gattttcgcc agcgtgccca
     1141 ttgcggtcgt agcccggccc ggtagcgaag tggccgcccg agcgtcgaag gcagccgtac
     1201 ggtttcgtac ctcttacgtg gccccggacc gtcatttttc cgtcatgccg gcaccagcct
     1261 ggacgatcct ggattccaga cgcaatccgg tcagtgcgac ggggttgcgg accggcgcga
     1321 ccctcgcgaa gaacgcgccg ctgtggtaat tctcccctcg cggacaccat ccgtgaatga
     1381 aggagtggct cgggcttgcg gcacagatca ggacttgcgt cttgcccggc tgttcgcttc
     1441 ggccggagtc tgatcctcta gaccaaggag ccaaccctga cacctcgtcc acgcgtaaag
     1501 cttaactctt ctccgccccc tgagcgttcc gagccggcat tgctcgcccg catccaggca
     1561 accctcgatg acgacaaagc cgaagatctg gtgacgatcg atctcaccgg ccggtcgccg
     1621 ctgacggacg ccatcgttgt ggccaccggg cgttcggcgc gccatgtggc ctcgctcgcg
     1681 gagaatctgg cgcggcgatt gaaagaatgg ggctatggca aggtccatct cgagggacta
     1741 ccccagggcg actgggtcct ggtcgacgca ggcgatgtgg tggtgcatat ttttcgcgct
     1801 gaagtccgag cctattatga tttagagggc atgtggtcgg tggatgaaac gagccatcgg
     1861 cgccgcctct ccgaacacgc cgattctgtc gcctgaacca atgcgcctca tcgtcgtggc
     1921 cgtgggccgg gcacgaaccg cgccggaaca gacgctgtgc gatttgcaat gggagcgggc
     1981 caccgcgttg ggcagaaagc tcggttttac caagcttgat ttcgcaattg tcgagacttc
     2041 gcatgcggga gatgtgctcg cgcgcatgga agaagaagcc aagcggctga caagccgggt
     2101 tcctgccggc gctcgtcgca tcgcgctgga cgaggcggga cggtctcttt ccagcgctgc
     2161 cttcgcaaaa caccttgcgc aattgcgcga ccggggagaa cgcgatcttg ccttcctgat
     2221 cggcggaccg gacggtctcg ccccggagtt gcgcgaaaac gcggaggaac gcctcgcttt
     2281 cggatgtcag acctggccgc atcttctggt gcgcgccatg cttgccgaac agatttatcg
     2341 cgcttttgcg attctctcgg gccatccgta ccatcgtggc cgcgcgaatt agcgctttat
     2401 taagcgtaac ccaggaaact gtggtcaata gccggaggcg gttgacgtct tggaaatcaa
     2461 atcgacacgc aaactcgaat ccgcatcgcc ggcgcggcgc gccggccgtg ccgcatcctt
     2521 gggaggcaac agcttctcca cggaggaaat cgcggatgcc aaacctgcca ccgctctgac
     2581 gggaaccggc cccatcgccg cggtcgacac cattctggcg cttcagggca tcgaggactc
     2641 gaccagcagc cgcaagcgca gcgtgagcca cggcgaacaa ttgctgacgc ttctcgacga
     2701 gctcaaagac ggtcttctgg cgggcggaat tccgcgcgcc gtgctcaatc gcctggcact
     2761 tgccatctcc aaacggcggg aaagctttgt cgatcccgca ctccagggcg ttctggacga
     2821 gatcgatctg cgtgcccggg ttgaactcgc aaagctggag caggccgagc ggccggccgt
     2881 ctagatcacg tttgcggaca tttctttcta tttgcccgcg catggccccg ccgctataac
     2941 cgcgccgccg atgggatgcg tttgacctga ggagagtgca tggcgaccga cctgccgcac
     3001 ggctaccggc cgtccgatga agaatctttt atgaacgagc gccagcgcga atattttcgt
     3061 cgcaagctcg tagcctggaa ggacgatatc cttcgcgaaa gccgcggaac cattcagaac
     3121 ctgcaatccg agcctacgcc caatgccgat cttgcggacc gcgccacgac cgaggccgaa
     3181 cgccagctcg agcttcgaac ccgcgaccgg caacgcaagc tgatcgcgaa aatcgactcc
     3241 gcgttgcgcc gcctcgaaga cggctcttat ggcttctgtg aggaaaccgg tgaaccgatc
     3301 tcgctgaaac gtctcgacgc gcgccccatc gcgacccttt cggttgaagc gcaggagcgt
     3361 cacgaacgcc gcgagcgggt ctatcgggac gattgattca tcccgctcga tcccggatcg
     3421 cgcggatgcg catccgggat gattttatct tccgtcaaat cagaacggcc agacaatatc
     3481 gaagaactgc tgtccgtaac ggggctgctg cacatcggtg atctggccgc ggccaccata
     3541 ggaaatccgg gcttcggcaa tctgggtatg gctgatcgta ttggtgttgg agatgtcttc
     3601 gggacgcacc acgcctgaca ccgacagctc gcgtacctca ttgttgacgc gcacttcctg
     3661 ctggccctgg atcaccagat tgccgttggg cagcacctgc gtcacgatcg cagcgacggt
     3721 aagattgatg gtctcggacc ggtccacggt cccagtgccg gtattggccg aatccgaccc
     3781 cagcttcacc aggctcgagg gatcgacgga tttcggaagt atgcccttca gacggctttc
     3841 aagccccagc agattgggcg cgcttgcatc ttccgatgcg gtggtggaac gcgacgtcgt
     3901 attggcgatc ttcgcctgat cgccgatcgc aatctgcacg gtcagaaggt caccgatctt
     3961 cgcggctctg ggatcgcgaa agaagctgcg cgaacctgct tgccatagcg aattcgcctg
     4021 cggcggcaga ttttcgatcg ccggctgagg cacctgaacc ggagcgccgg cgacaatgtt
     4081 cggctgcaca tggatcgggc tcaaggccgg cggcttgccg atttgctcga tcttgtcgac
     4141 ggtgctggcg caggaggtga gcaaaaggct tgcggtacag gcggcagaag ccaaagcgcg
     4201 ccgggggagg atatgaatca tcttggatgt ccctttgtta tttcgcgatg gcgacttcgt
     4261 tggtcttcgc cgaagcatcg gccgggccga cacggacaac gccaggagcg gtgaccaccg
     4321 cgaccacctg gcggtaggat gtggggttga gcaccgcgat ggaatcgcct tcgccccctt
     4381 cggcaagggc gcggcccacc gccgtcagcg acattcccgg cgcgtcgaac accatcgtga
     4441 cagtcgcacc tttggcgacg agtgccggac gcttgagatc gctcaggcgc aagacttcgc
     4501 cggcgcgaag cgcccgcttg gcctctttgc cttcgatatc gctgagattg tggatgaaag
     4561 attcgttcat ccgcagaacg gggaacgatt ttgtcgtcag gtcgccatcg gcgatcacgg
     4621 ccccgcgcgc gatgtcgtgc gccggaacga cgagttcggt ctgattgtcc gcgtcttgcg
     4681 cgtaggcgct gaaggcgacg gtgcagaagg caagggcgag aacgaagcgc aaggtcatgg
     4741 cggttagctc atcttcgagg tgatttgcag catctgatcg gcggccgaga tgactttgga
     4801 gttcatctca taggcgcgct gggcagtgat cagatcggtg atctcctgca cggcattgac
     4861 gttcgaggtt tcgagatatc cctgttgcaa cgtgccgtaa ccgggcgatc ccggaagtcc
     4921 cgtcagcacc gcgccagagg caggtgtctc cgtgtacaga ttgtcgcccg tggcctgcaa
     4981 tccaccctcg ttcggaaacc gcgcaagctc aagttggcca agggtctgcg gcgcattttg
     5041 tcccgcaatc gtcgcctgca cctggccctg agcgttgatg gtgaccgaag tggcatcctg
     5101 cgggatggcg accccgggcg cgaccacata gcccttgtcg gtgaccaatt ggccttccgg
     5161 ggagagcgag aaagcgccgg cccgggtgta ggcgtcggtt ccgtccggca tctggacatg
     5221 gaaatagcct ttgccattga tcgcgagatc gtagggattg ctggtactgt tgagattgcc
     5281 ctgggcggta atccgatagg tcgccgcggt ccgcacaccg acgccgatct ggatgcccgc
     5341 gggcaggact gcgccggtat cggcggacgc cgcgccggga cgttccacat tctggtagag
     5401 caggtcctgg aattccgccc gctgcatttt gtacgccgtg gtgttcatgt tcgcgagatt
     5461 gttcgcgatc acttccacat tgttctgctg cgccagcatg ccggtcgccg cgatgctcaa
     5521 agcccgcata atcttaactc ccttagcttg caggaacggc gccgagcttc tcgatcgcct
     5581 gtttcaaaag gtcttcaccc gatttggtca ggtccgcgct cgtctgatag gcgcgcatga
     5641 tctcgatcat gcggctgatt tcgatcatcg gctcgacgtt ggatttctcc atcatgccct
     5701 ggcgtacgcg cgcggtagtg gatgcctggg ccgcctgtcc gccggcgtcg taattgcttg
     5761 cgcccgtttt cttcaatgcg cgttcattgg cgaattcgac gacgcggagc tttccgagaa
     5821 gctgtagatc ggtactgagt gtaccgtcag accccacctt gagatcgccg tcctgcggct
     5881 ggagtgtgat cgcaccgccg tcgctctgga ccggatagcc gtcgtcggtc acgacttggc
     5941 cctgatcgtt cagactgaaa tggccattgc gcgtgtagcg ttcgccatcg ggcgtctgca
     6001 ccacgaaata gccattgccc gcgatggcga gatcgagcgg cgcccccgtg ggatcgaagc
     6061 gtccttcgga gagatcgcgg gcaacgccgc ggtcaagcac aaaggccaca
//
LOCUS       DASYEG010000277        11072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4019687, whole genome shotgun sequence.
ACCESSION   DASYEG010000277 DASYEG010000000
VERSION     DASYEG010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 11072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11072
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4019687"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VGM72_11730"
     CDS             <1..657
                     /locus_tag="VGM72_11730"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY4265980.1"
                     /translation="KIAEGRAMLQAKRSPAPYVRHLFDQFAERYDRQMLDDLSYCAHR
                     ILRGLADMLVATEPDMLDVLDLGCGTGLAGDAFKDVARRLDGLDLSPGMIERARARGI
                     YDALSVGDVENLDVCKPAVYNLIVSADTLVYLGDLGAVFRGVHRRLRPDGFFLFTVEK
                     RTEAGYRLGPKRRYAHSESYLREEAERAGFEVMGLLECSPRRDANVPVAGFAAAFQRR
                     "
     gene            746..1114
                     /locus_tag="VGM72_11735"
     CDS             746..1114
                     /locus_tag="VGM72_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265981.1"
                     /translation="MTRGILLTAAALAMLAAPAFAQDRCSAPLAPTVPDGKTASIAQL
                     SQSAKDVVAFIKASDDYQTCLLAAIAEQDKLAKDPKNPPDPAIKKGLETKGDANQKEK
                     ERVGGEYNKSAAAYKTAHPK"
     gene            1229..1837
                     /locus_tag="VGM72_11740"
     CDS             1229..1837
                     /locus_tag="VGM72_11740"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY4265982.1"
                     /translation="MSEALSRRTERRTRQRAANRNAILEAARRVAAREGAGELSLRAV
                     AGEAGYAPASVYEYFRNRAELVLALAAEDMGSLARALREAGLARDGGPGVLAAAAKIA
                     LGLMRGSGALPAAVATLEGGAAPPEAERVFNGRLIAALTAFAEAAGRVPKTREEQADV
                     VLAAAAVTGLAVLVRAGRLKTLGFDEDALLDRLDSRFAGPSP"
     gene            1923..3164
                     /locus_tag="VGM72_11745"
     CDS             1923..3164
                     /locus_tag="VGM72_11745"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEY4265983.1"
                     /translation="MDVQRRDFLLTGTIAAAAVAAAASPALAQTAPEGPGQERGGSVR
                     PGDENIQYEGGKEVKKLKIVNTAELEIEAEKVLPKGGYGYIFGGSGGEYTKRENLRAM
                     EAIGLEPHFLAGVLKPDLSATILGHKLPFPILVPPMGSHGLAHVSKEIGTAQAVADMK
                     TLMILSMQSNVSLEDVAKANPGPKWMQLYFPADRGYAREVIMRAKAAGYTAIVPTIDS
                     TLAYPRDNNIRNNFRIPVSLGKGNAPLDEKDPVKAARIMGVRKVDLNWDDMLWIKQET
                     GLPVVVKGVMSPVTAMQAVERGIDAVYVSNHGGRALDGVPAAISVLPRIADAVKGRVP
                     IVYDSGIRRGQDVFRALALGADVVACGRPILYGLALGGHLGAQSVLEYLRDDLYIVMQ
                     LAGTPNIRSITRDHIAPPRLA"
     gene            3167..5281
                     /locus_tag="VGM72_11750"
     CDS             3167..5281
                     /locus_tag="VGM72_11750"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4265984.1"
                     /translation="MSKLRPILFSCAASALWPMVSAAQQANAPAGPEVYVNCQYRVAA
                     IFPTAPRVRDIAYTNGGRTVPARQFYVERGRDRFSVIVADFTNAAPAIDDEIVENAAA
                     ALRLRGETRVQFPEDYSPGIPGRQLNVFDTNGRQHLASVYMADHRLYLTETDAAPGDF
                     AALQFEQSVLLIDANGGDLNNVNVANRTHYTCETQPPIAESAGDLVSAAIAAEGGAPA
                     LRALANLSVRGTAKFWEPGQSFTAGGEPRFLGTASFTIAWNLASGMARTEWDRDQQYP
                     PPAAKLKYTETLSPATGFVTDTNGAQPMSSIRIAAQLRELERASPRLLLKAMSDPANV
                     RGAEFQQLGDRSLPAVSFMDGGANFLILFDPATHLPAAIRTRDDDNISGDSNYDLVLS
                     GWTQVSDARVAKSLSYRINNVEVARLDYDTVTGNAPPGGYVFAVPAAIQPAVKSPAAS
                     GVPYQWVLRRLFLTRFTDSDTIIYPAGGGLKLVELAPNVQHVQGGTANNLIVAMKDYL
                     VVFDAPYGELQSRWTIDAAKAKYPGKPIKYLVLTHHHMDHAGGMRTYVAAGASVVVPS
                     ESVDYIEKAIKAPHTLVPDELQKNPRNPVVYGVFENMTIKDGTAEIRLYNLSAASNAE
                     APRNTNPHVDGMLIGHIVDRKLIYVTDLISPRGPIARSPETIAVGKSLREFDIRDPDL
                     IFAGGHGTTVKEADIAGALAAN"
     gene            complement(5852..6427)
                     /locus_tag="VGM72_11755"
     CDS             complement(5852..6427)
                     /locus_tag="VGM72_11755"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY4265985.1"
                     /translation="MARAAAKMRRCLIVSLVAFPAAMAIAGCNRQSATATSPASPAQA
                     AAVPAAPALHLPFPIRDYMRASVEIPADGLWAAQGASMLSDDEWLLADQDSADLAAAA
                     TFMNIPGNGKDDEKWVTNADWQMWAADVQKTALQIRDAVKAKDLMKLSDGADHLAETC
                     QACHDKYRPETPSDGIARYPFYPKRIIAPKK"
     gene            complement(6513..7043)
                     /locus_tag="VGM72_11760"
     CDS             complement(6513..7043)
                     /locus_tag="VGM72_11760"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY4265986.1"
                     /translation="MHLTGICARNILSALTIGLATAASAASAPTPFKPATGVITVHDV
                     SLEMAKAAAEATIGECRAKGFHTAVTVVDRAGQVIVSMRDELATPQMAEMSRRKAYTA
                     RMFRVSTADWAKRTRDDPSVAPQRDLPDVLALAGGVPIKMGEETIGAVASSGSNLAQD
                     DACAHAGATKAEGMMK"
     gene            7172..8182
                     /locus_tag="VGM72_11765"
     CDS             7172..8182
                     /locus_tag="VGM72_11765"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4265987.1"
                     /translation="MWQTRIRAALLIFAAVVFAWANGRAEAAGPIPFKVGIAAPTVNM
                     LPLWMGQSAGLFKARGLDVTIVNTDGGSRGLSEVGAGRLQAMVVGLSAVIDANGKGGD
                     YRLIASGANTMSFRFFGAKGVASAAFLPGKKIGVSAFGSESDSAASLALRQLGLARRQ
                     VSVVEAGGTLKRLKALQSGALAATALNEPADTLAQRAGLPLLADLKANLPWIFTAIVM
                     DRRALGAHRDHVKDFLRAYVEAIHLALSDPKRAKAVLASEFKDFPPDVVEATYADFKA
                     RVPRDAAPSREGAETMLRELPGLGATVKSSNVADYVDASLIEELRREGFFDAMKSKYR
                     VE"
     gene            8190..9527
                     /locus_tag="VGM72_11770"
     CDS             8190..9527
                     /locus_tag="VGM72_11770"
                     /inference="COORDINATES: protein motif:HMM:NF014697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HEY4265988.1"
                     /translation="MLRGLTRIFVRYAERYIPDPYLYALILTFVTAAAAFVWTPAGTG
                     KIIASWYDGIWAILAFAMQMALILSTGVALAEAGPVKRGLEKLAAIPSRQSGAAVTMF
                     LVSGFANWLNWGFGLVAGALLAREIAKRLRDVDFGFLVAIAYMGNMVWASGLSSSIAL
                     ATATPGSALNIVEKVTGKVAGLNETIFTAYNLVPTILVFILLPIVLVAIAPAPAEMKR
                     VDPQILHRQDAPSEIPSSEKNFAAAIENSPLFTLALLAMGVAYEANVAVTRGFTLDID
                     GMIFIALMLGLLFHWRPIRYVRAFNRAARTVGPVLLQFPLYGGIMGMMTGTGLAAVIA
                     QGFVAFSTHGTLPFWSFVSSNVITLFVPSGGGHWAVQGPFMVPAAVSLQVDPALTAMA
                     TAMGEQTANMIQPFWALPVLAIAGLGIRDIMGYCVMALLVGLLLYGGALLLFA"
     gene            9546..9998
                     /locus_tag="VGM72_11775"
     CDS             9546..9998
                     /locus_tag="VGM72_11775"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY4265989.1"
                     /translation="MAALVNRRTITIEWGQCDPAGIVFYPNYLIMFDTSTGLLFARTG
                     LSPAEMRARYGIVGLPLVEQGTKFFVPCRFDDELIVESEVGEWGRSSFTVRHRVMKGR
                     DVAVEGFEKRVWAIADPERAGRIKPEPIPREVMASLSDPTGATRIANP"
     gene            10015..>11072
                     /locus_tag="VGM72_11780"
     CDS             10015..>11072
                     /locus_tag="VGM72_11780"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY4265990.1"
                     /translation="MRVAILGAGPAGLYLAYLLKRARPDFAISMYEQNPPDATFGFGL
                     AFSERALEFLRSEDADTLAALAPALERWNDSVLSLNGKEVRIDGMGYAGIGRLRFLEI
                     LQARARSVGIEPVYGRSLRSLAELEPCDAIVGADGVNSLVRGSGEAGFGTAIEYFSNR
                     FVWFGTPKRFEALSHTFIDTPHGPFNAHHHRHARDMSTFVVEVDAATFFRCGFDTMDA
                     GDAQTYCARVFRDTLSGAPLISNKSIWRQFPKITNARWSRGNRVLIGDALHTAHFSIG
                     SGTRLAMEDAIALSRALATYGSDRPAAFAAYEAARKPAIAKLVAAADCSAQWYENFAA
                     HMRLSPLDFAMSYITRSGR"
BASE COUNT         1936 a         3525 c         3496 g         2115 t
ORIGIN      
        1 aaaatcgcgg aaggacgtgc gatgctccag gcgaagcgtt cgcctgcgcc ctatgtgcgc
       61 catctcttcg accagttcgc ggaaagatac gacaggcaga tgctggacga tctgtcctat
      121 tgtgcgcatc gcattctgcg cgggcttgcg gacatgctgg ttgcaaccga accggacatg
      181 ctcgatgtac tggatctggg ctgcgggacg gggctcgcag gcgatgcatt caaggatgtg
      241 gcgcggcgtc tcgatgggct ggacctctcg cccggcatga tcgaacgcgc gcgcgcccgc
      301 ggaatctatg atgcgctcag cgtcggcgat gtcgaaaatc tcgacgtttg caagcctgcg
      361 gtttacaacc tcatcgtctc ggcggatacg cttgtctatc tcggcgatct cggcgcggtt
      421 tttcgcggcg tccatcggcg gctgcggccg gacgggtttt tcctgttcac ggtggaaaag
      481 cgaacggagg ccggctacag gcttggtccc aaacgccgtt acgcccacag cgaatcctat
      541 ctgcgcgaag aagccgaacg cgcgggcttc gaagtgatgg gactcctgga atgttcgccc
      601 cggcgcgatg ccaacgtgcc ggtggcgggc ttcgcggctg cgtttcagcg ccgctgaggc
      661 ggccaaaccc tggcaaattc gctttgagcc cctgagcggt ttgtgctcta ttccgcgccg
      721 atatggggta ccgaggagca cacgaatgac ccgtggaatt ctgttgactg ccgcagcgct
      781 cgccatgctg gcagcaccgg cttttgccca ggacaggtgt tcggcgccgc tcgcccccac
      841 tgtgcccgac ggcaagacgg cgtcgattgc gcaattgagt cagtccgcga aggacgtcgt
      901 cgccttcatc aaggcatcgg acgattacca gacctgtctt ctggcggcga tcgccgagca
      961 ggacaaactg gcgaaggacc ccaagaatcc gcccgatccg gccatcaaga aagggctcga
     1021 gaccaaaggc gatgccaacc agaaggaaaa ggaacgcgtc ggtggtgaat ataataagtc
     1081 cgcggccgct tataagaccg cccatccgaa atagatccgt tacgcatcgt tgccgcgcgg
     1141 ccctgggtga agatcctggg gccgcttttg tttgtgtgcg cccgaagggc caatcgtcct
     1201 taggaccgaa tcgtctatca tgcgccgcat gagcgaagcg ctttcccgcc gcaccgaaag
     1261 acggacccgc cagcgcgcgg ccaaccgtaa cgcgattctc gaagccgcac gccgcgtggc
     1321 ggcacgcgaa ggcgcgggcg aactctcctt gcgcgcggtg gccggagaag caggttacgc
     1381 gccggcctcg gtctatgaat atttccgcaa ccgtgccgaa ctggtgctgg cgcttgccgc
     1441 cgaagacatg ggaagtcttg cgcgggccct gcgcgaagca ggattggcaa gggacggcgg
     1501 accgggcgtg ctggccgcgg ccgcgaaaat tgcgctcggc ctgatgcgcg gcagcggcgc
     1561 gctgccggcc gccgtggcga ccctggaggg cggcgcggcc cctccggaag cggaaagggt
     1621 gttcaacggc agacttatag ccgccctcac cgcgtttgcg gaggctgcgg gccgcgttcc
     1681 caaaacgcgc gaggaacagg ccgatgtcgt gctcgctgcc gcggcggtca ccggccttgc
     1741 ggtgctggtg cgggccggcc ggttgaagac cctcggtttc gacgaggatg cgcttctgga
     1801 ccggctggat agccgctttg cagggcccag cccttaaatc gcgttgcggc ctcatggcgg
     1861 ttttcggtta gactgaacgc aaggctcggg gtcgatccgg gcgttcctcg ggaggatttg
     1921 tcatggacgt gcagcggcgc gattttcttc tgacgggcac gatcgcggca gcggcggtcg
     1981 cggcggcggc gagcccggct ttggcgcaga ccgcgcccga gggtccgggg caggaacgcg
     2041 gcggcagcgt gcgccctggc gacgagaata tccaatatga aggcggcaag gaggtgaaga
     2101 agctcaagat cgtcaacacc gccgaacttg agatcgaggc cgagaaggtc ctgcccaagg
     2161 gcggctatgg ctacatcttc ggcggttcag gcggggaata caccaagcgg gagaatctgc
     2221 gcgccatgga ggccatcggc ctggagccgc atttcctcgc cggcgtgctc aagcccgatc
     2281 tgtcggccac gatcctgggg cacaaattgc cgttcccgat tctggtgccg ccgatgggca
     2341 gccacgggct tgcccatgtg tcgaaggaga tcggcaccgc gcaggcggtg gccgacatga
     2401 agacgctgat gatcctttcc atgcaatcca acgtctcgct ggaggacgtg gcgaaagcca
     2461 atcccggacc gaaatggatg cagctctatt tcccggccga ccgcggctat gcgcgcgagg
     2521 tcatcatgcg cgcgaaagcg gcgggctaca cggcgatcgt ccctaccatc gattccacgc
     2581 tcgcctatcc gcgcgacaac aacatccgca acaatttccg cattccggtc tcgctcggca
     2641 aaggcaatgc gccgctggac gagaaggacc ccgtgaaggc cgcgcgcatc atgggcgtgc
     2701 gcaaagtgga cctgaattgg gacgacatgc tgtggatcaa gcaggagacc ggccttcccg
     2761 tcgtggtcaa gggcgtgatg tcgccggtca ccgcgatgca ggcggtcgaa cgcggcatcg
     2821 acgcggtcta tgtctccaac catggcgggc gcgcgctcga cggtgtgccg gcggcgatca
     2881 gcgttctgcc gcgcatcgct gatgcggtga agggccgggt gcccatcgtc tacgattccg
     2941 gcataaggcg cggacaggat gtgttccgcg cgctggcact cggcgcggac gtggtcgcgt
     3001 gcggccgtcc gattctttat gggttggcgc tgggcggcca tctcggcgcg cagagcgtgc
     3061 tcgaatatct gcgcgacgat ctctacatcg tcatgcaatt ggccggcacg cccaacatcc
     3121 gctcgatcac gcgcgaccat atcgccccgc cgcgcctcgc gtagccatgt ccaagcttcg
     3181 tccgattctt ttttcgtgcg ccgcttcggc cctatggccc atggtatcgg ccgctcagca
     3241 ggccaacgct cctgccggcc cggaggtcta cgtcaattgc caatatcgcg tggccgcgat
     3301 tttccccacc gcgcccaggg tccgcgatat cgcgtatacc aatggcggca ggacggttcc
     3361 ggcgcggcaa ttctacgtcg aacgcggccg cgacagattc agcgtgatcg ttgcggattt
     3421 caccaatgcg gcgcccgcca tcgatgacga gatcgtcgag aatgcggcgg cggctttgcg
     3481 gctgcgcggc gaaacccgtg tccagtttcc cgaagactat tccccgggca ttcccgggcg
     3541 gcagctgaat gtcttcgaca ccaatggccg ccagcatctc gcgtccgtct acatggccga
     3601 tcatcgcctt tacctcaccg aaaccgatgc ggcgccgggt gatttcgcag cccttcagtt
     3661 cgagcagtcc gtgcttctga tcgatgccaa tggtggcgac ctcaacaatg tcaatgtcgc
     3721 caacagaacg cattacacgt gcgaaacaca gccgcccatt gccgagagcg ccggcgatct
     3781 cgtcagcgcg gccattgcgg cggagggagg cgcgccggcg ctgcgcgcac tcgcgaacct
     3841 atccgtcagg ggtaccgcaa aattctggga accgggacaa tccttcaccg ccggcggcga
     3901 gccgcgtttc cttggcaccg cgagcttcac gatcgcctgg aatcttgcga gcggcatggc
     3961 gcgcaccgaa tgggatcgcg atcagcaata tccgcctcca gccgcgaagt tgaaatatac
     4021 cgagacgttg tcgcccgcga caggcttcgt caccgacacc aacggcgccc agccgatgtc
     4081 gagcattcgc atcgccgcgc agctgagaga gctggagcgg gcgagtccgc gtcttctgct
     4141 gaaggccatg agcgatcccg ccaatgttcg cggcgcggaa ttccagcagc tcggcgaccg
     4201 ctcgctgccg gcggtttcct ttatggatgg gggtgcgaat ttcctcatcc tgttcgatcc
     4261 cgcgacgcat ttgcccgccg ccatccgcac ccgtgacgat gacaacatca gcggcgattc
     4321 gaattacgat ctggtgctct ccggctggac acaggtctcg gacgcgcgcg tcgccaaatc
     4381 gctgtcctat cggatcaaca atgtcgaggt ggccaggctc gactatgaca ccgttaccgg
     4441 caatgcgccg ccggggggat acgtgtttgc ggttcccgcg gcgattcagc cggcggtcaa
     4501 atctccggcc gcaagtggcg ttccctatca atgggtgctt cgccgcttgt tcctgacgcg
     4561 cttcacggac agcgacacga tcatctatcc cgcgggcggt ggcttgaagc tcgtggaact
     4621 cgcacccaat gtgcagcacg tgcagggcgg caccgcgaac aatctgattg tcgcgatgaa
     4681 ggattatctg gtcgtgttcg atgcgcccta tggcgaactg caatcgcggt ggaccatcga
     4741 tgcggcgaaa gcgaaatatc caggaaagcc gatcaaatat ctcgtgctca cccatcacca
     4801 tatggatcac gcgggcggca tgcgaaccta tgtggcggca ggtgcatcag tcgtcgttcc
     4861 cagcgagagc gtcgattaca tcgaaaaggc catcaaggcg ccgcacacct tggttccgga
     4921 cgaacttcag aagaatccgc gcaacccggt agtctacggc gtcttcgaaa acatgacgat
     4981 caaggacggc acggcggaaa tccgtctcta caatctctcg gcggcgtcga atgcggaagc
     5041 gccgaggaac acaaatccgc atgtcgatgg catgctgatc ggccatatcg tcgaccgcaa
     5101 gctcatctat gtgaccgatc tcatttcgcc gcgcggtccc attgcgcgtt cgccggagac
     5161 gatcgcggtg ggcaagagct tgcgcgagtt cgacatccgc gatcccgacc ttatttttgc
     5221 cggcgggcac ggcacgacgg tcaaagaagc cgacatcgcg ggcgctttgg ccgcgaacta
     5281 gacgccgcat cctgccggcc gtccatggat aatcatctct acacggatta tatccgcgca
     5341 gcgtttcgcg caacgcgagg ggtggcgtga ggcgccgtct ccggcgtcaa cgcgcgtttg
     5401 aaaaaaaggt gccgatttcg gcccctcacg cctggcggtt ttgggcgtcg cgctttccga
     5461 tttcgcaagg ccgcttttca aagcggccgg gcttatcggc atcccgtcct cccgccggcc
     5521 ctttctgtcg aaaaggtagc ggggcggccg gaaagcgccg ccctcgcatc cttccttgac
     5581 aggccctcct gaaagagcgc tgccgccgaa gtttttcccg cccgtccgtc aatcggacag
     5641 aagatgcaaa cgccagccgg aaatctcacg ctgaagacgg tcgacaacgc cccctcatca
     5701 ggttccggct gtctgcatgt gaggacgtat tcctggaaaa caagaggagc gactcgctgc
     5761 tgcgaaatgc catggcctct ccatgagcga gacacggcaa tgtccggcgg cagaaagaac
     5821 gacggaatat ccaagcaaaa tatgcgagcg gctatttctt cggggcgatg atccgcttgg
     5881 gatagaacgg gtagcgcgcg atgccgtctg acggcgtctc ggggcgatat ttgtcgtgac
     5941 aggcctggca agtctcggcc agatgatccg cgccgtccga cagtttcatg aggtctttgg
     6001 ctttgacggc atcacggatt tgcagcgccg tcttttgaac gtcggccgcc cacatctgcc
     6061 aatcggcatt ggtgacccat ttctcgtcat ctttcccatt gccgggaata ttcatgaatg
     6121 tcgccgccgc cgcgaggtcg gccgaatcct gatcggccag cagccattcg tcgtcggaga
     6181 gcatggaagc gccctgcgcc gcccacaatc cgtccgcggg aatttccacc gacgcgcgca
     6241 tgtagtcccg gatcggaaac ggcagatgca atgccggcgc cgccggtacg gcggccgctt
     6301 gggccggcga agcggggctc gtcgctgtgg cggattgccg attgcaaccc gcgatcgcca
     6361 tcgcggcagg gaacgctacc agcgaaacaa tcaaacaacg ccgcattttc gcagctgctc
     6421 gtgccatgaa accctcctta caagcgcaaa cgcattttcg cgatttcgcg cttttacgcc
     6481 gccttccacc ggtgggcaac caccggcccc ggctacttca tcatcccttc ggctttggtt
     6541 gcgccggcat gggcgcaggc atcgtcctgg gcgaggttgg aaccggaact cgcgaccgcg
     6601 ccgatggttt cctcgcccat tttgatggga acgccgcccg cgagcgccag cacgtcgggc
     6661 agatcgcgct gcggcgcgac ggaagggtcg tcgcgcgtgc gcttcgccca atccgccgtg
     6721 ctgacgcgga acatgcgcgc ggtataggcc ttgcgccgcg acatctcggc catctgcgga
     6781 gtcgccagtt cgtcgcgcat gctgacgatc acctggcccg cgcggtcgac caccgtgacg
     6841 gcggtgtgga agcccttggc gcggcactcg ccgattgtcg cctcggcggc ggccttggcc
     6901 atttccagag agacatcgtg cacggtgatg actccggttg ccggcttgaa tggggtcggc
     6961 gcgctggcgg cgcttgcggc cgtcgccaac ccaatcgtca gcgccgacag gatatttcgg
     7021 gcgcagattc ccgtcagatg catgcaattc ctccgtgcgg tctctggttg cctgagttta
     7081 cgggaaaagc ccgcaagttt tgaagcccgc cagagcccgc gctggtctgg ccgcgtgttc
     7141 cttgttatgc agggagccgg ggagaaacgc catgtggcaa acgcgaattc gcgccgctct
     7201 gctgattttc gcggcggtgg ttttcgcatg ggccaacggc cgggccgagg ccgctggtcc
     7261 cattccgttc aaagtcggca tcgcggctcc cacggtcaac atgctgccgc tgtggatggg
     7321 acagtcggcc ggtctgttca aagcgcgcgg ccttgacgtc acaatcgtga acaccgatgg
     7381 cggcagccga ggcttgagcg aagtgggtgc gggccgtttg caggcgatgg tcgtgggtct
     7441 gtccgccgtc atcgatgcca atggcaaagg cggggactac cgtctgatcg catcgggcgc
     7501 gaacacgatg agctttcgct tcttcggcgc gaaaggcgtt gcgagcgcgg cgttcctgcc
     7561 cgggaaaaag atcggtgtca gcgccttcgg ttcggaatcc gacagcgccg cgtcgctcgc
     7621 gctcaggcaa ttgggactgg cgcgccgcca ggtgagcgtg gtcgaggcgg gcggcacgct
     7681 gaagcggctg aaggcgctgc aatccggagc gctcgccgcc acggccttga acgagcccgc
     7741 cgatacgctc gcccaacgcg caggcctgcc gttgctggcc gatctgaagg cgaacttgcc
     7801 ctggatcttc accgccatcg tcatggaccg acgcgcgctt ggcgcccatc gcgatcacgt
     7861 gaaggatttc ctgcgcgcct atgtcgaggc gatccatctg gcgctctccg atcccaagcg
     7921 cgcgaaggcg gtgctggcct ccgagttcaa ggattttccg cccgatgtgg tcgaggccac
     7981 ctatgccgac ttcaaggccc gcgtgccgcg cgatgccgcc ccttcgcgcg agggggcaga
     8041 gaccatgctg cgcgaattgc ccggtctggg cgccaccgtc aaaagctcca atgtcgccga
     8101 ttatgtggat gcgagcctga tcgaggaatt gcggcgtgaa ggttttttcg acgcgatgaa
     8161 atccaaatac cgcgtcgagt gaagcgatca tgctccgggg gctcacgcga atcttcgtcc
     8221 gctatgccga gcgttacatc cccgatcctt atctttacgc gctgatcctg accttcgtga
     8281 cggcggcggc cgcctttgtc tggacgccgg ccggcaccgg caagatcatc gcctcctggt
     8341 atgacggcat ctgggccatt ctcgccttcg ccatgcagat ggcgctgatc ctgtccacgg
     8401 gcgtcgcgct cgccgaagcg gggccggtga aacgcgggct tgagaaactg gccgcgattc
     8461 cctcccgcca atcgggtgcc gcggtcacca tgttcctggt gtcgggattc gcgaactggc
     8521 tgaattgggg atttggcctg gtggccggcg cactgctcgc gcgcgaaatc gccaagcgcc
     8581 tccgtgacgt ggatttcggc tttctcgttg ccatcgccta tatgggcaac atggtgtggg
     8641 cgtccgggct ttcgagttcg attgcactgg ccaccgcaac gccgggaagc gcgctcaaca
     8701 tcgtggagaa ggtgacgggc aaagtcgcgg gcctgaacga gaccatcttc accgcctata
     8761 atctcgttcc cacgattctc gttttcatcc tgctcccgat tgtgctcgtc gccatcgcgc
     8821 cggccccggc cgagatgaag cgcgtcgatc cgcaaattct tcaccgtcag gatgcgccat
     8881 cggaaatccc atcgagcgag aagaatttcg ccgctgcgat cgagaattcg cctttgttca
     8941 ctctggctct ccttgccatg ggcgtcgcct acgaagccaa tgtcgccgtc acgcggggct
     9001 tcacgctcga catcgacggc atgatcttca tcgcgctgat gctcgggctt ctgttccatt
     9061 ggcgccccat ccgttatgtg cgcgccttca accgcgccgc gcgcaccgtg gggcccgtcc
     9121 tgctgcaatt ccccctctac ggcggcatca tgggcatgat gacgggcacg ggcctcgccg
     9181 cggtgatcgc gcaaggtttc gtcgcgtttt cgacgcatgg gacgctgccg ttctggtcat
     9241 tcgtgtcgtc caacgtcatc accctgttcg tgccctccgg cggcggccac tgggccgtgc
     9301 agggcccctt catggtgcct gcggcggtga gccttcaggt cgatccggcg ctgaccgcca
     9361 tggcgaccgc gatgggcgaa cagaccgcca acatgatcca acccttctgg gcgctgccgg
     9421 tgctggcgat tgccggcctc ggcatccggg acatcatggg ctattgcgtg atggcgcttc
     9481 tggtcggtct tttgctgtac ggtggcgcgc tgttgctatt cgcctgaccc tggcaggatc
     9541 gtttcatggc cgctctcgtc aatcgccgca caatcaccat cgaatggggt caatgcgacc
     9601 ccgcgggcat cgtgttctat ccgaactatc tcattatgtt cgacacttcg accggacttc
     9661 tgttcgcgcg caccggcctc tcgccggcgg aaatgcgcgc gcgttatggg atcgtcggtc
     9721 tgccgctggt cgaacagggc acaaaattct tcgtgccctg ccgcttcgat gacgaattga
     9781 tcgtggaaag cgaggtgggc gagtgggggc gttcgagttt caccgtgcgc catcgcgtga
     9841 tgaagggccg cgacgtcgcg gtcgaaggct tcgaaaaacg cgtctgggcc attgccgatc
     9901 ccgaacgcgc cggccggatc aagccggaac ccatcccgcg cgaggtgatg gcaagcctgt
     9961 ccgatcccac cggcgccacg cggatcgcga atccatgaac cggcgcttgc cctgatgcgg
    10021 gtcgcgattc tcggcgccgg gcccgccggg ctttatctcg cctatctctt gaagcgcgcc
    10081 cggcccgatt tcgcgatttc gatgtacgaa cagaatccgc ccgatgcgac cttcggtttc
    10141 gggctcgcct tttccgaacg cgcgctcgaa ttcctgcgca gcgaggatgc cgatacactg
    10201 gccgcgctcg cgcccgcgct ggagcgctgg aacgacagcg ttctctctct gaacggcaag
    10261 gaagtccgca tcgatggcat gggctatgcc ggcatcggcc ggttgcggtt cctggaaatt
    10321 cttcaggccc gggcgcgaag cgtcggaatc gaacccgtct atggccgcag cctccggtcg
    10381 ctggcggaat tggaaccttg cgacgcgatc gtgggcgccg acggcgtgaa ctcgctggtg
    10441 cgcggcagcg gcgaagcggg cttcggcacg gccatcgaat atttctccaa tcgcttcgtc
    10501 tggttcggga cacccaagcg tttcgaggcg ctgagccaca ccttcatcga tacgccgcac
    10561 ggccccttca acgcgcatca tcatcgccat gcgcgcgaca tgagcacgtt cgtcgtcgag
    10621 gtggatgcgg cgacgttttt ccgctgcggc ttcgacacga tggacgcggg cgacgcgcaa
    10681 acctattgcg cgcgcgtatt ccgggatacg ctgtcgggcg cgccattgat ctccaacaaa
    10741 tcgatctggc ggcaatttcc gaaaatcacg aatgcgcgct ggtcgcgggg aaaccgcgtg
    10801 ctcatcggcg atgcgctgca taccgcgcat ttctccatcg gctcgggcac aagattggcg
    10861 atggaagacg ccatcgcgct ttcacgcgcg cttgcaacct acggctcgga cagacccgct
    10921 gcgttcgcag cctatgaggc ggcgcgcaaa cccgccatcg cgaaacttgt cgccgcggcc
    10981 gattgcagtg cgcaatggta tgagaacttc gccgcccaca tgcgattgtc cccgctcgat
    11041 ttcgccatga gctacatcac gcgttcgggc cg
//
LOCUS       DASYEG010000278         5522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1120416, whole genome shotgun sequence.
ACCESSION   DASYEG010000278 DASYEG010000000
VERSION     DASYEG010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120416"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VGM72_11785"
     CDS             <1..538
                     /locus_tag="VGM72_11785"
                     /inference="COORDINATES: protein motif:HMM:NF014722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein adenylyltransferase SelO family protein"
                     /protein_id="HEY4265991.1"
                     /translation="AALKDAREAIGAFTVRFESAYTEEMRRKLGLLQSRAGDLALAQD
                     LLERMARNSADFTLTFRRLCDAAADPARGAPVRALFADPVAFDEWAVTWRQRLAEDGG
                     LDRECSAAMRAANPLFIPRNHRVEQAISAAVNDNDFVPFEMLLTVLSSPYADQPGHEI
                     YAEPPRPDEIVQQTFCGT"
     gene            618..1085
                     /locus_tag="VGM72_11790"
     CDS             618..1085
                     /locus_tag="VGM72_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265992.1"
                     /translation="MIQNRRMRRMEVRERASATIMRRPSLPRAAFVFLALAALAIQLF
                     VVQTHIHIPQAQGRSESVSIVTLAKTLIAGPQDQGAGQQANLPRDKYPINEDPSNCPL
                     CQELVHSGQFVQSVAVLSHISFWVSVHFIPLSEALPRLFIVSHSWQGRAPPQH"
     gene            1217..3235
                     /locus_tag="VGM72_11795"
     CDS             1217..3235
                     /locus_tag="VGM72_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4265993.1"
                     /translation="MRSQVFSAALTGASLFVLQPAFAGQAPTTNPDAVPEQVVITASP
                     FGENAIDQSASVSQVTREQLLNSGGIGLGDVLKDVPGVTSSGFSAGTSRPIIRGLGAT
                     RVRVTENGLGSHDVADVGDDHAVPIDPLASLDVEVLRGPATLRYGSQAIGGVVNAINN
                     RIPLDVGEGSNVESFAGMSSGNLERLGGGLADYREGNWAFHADGVIRGADDYDTPQGT
                     QINTFAFGRGYALGGAYIGDGGTSGGLGYNRYIAHYGIASEPGDPERKHIDLDQKNYN
                     SAMRFMAPLPGITSLNVTGGYSNYRHDEVTDADGISALFTNKEWEGRAEAVHEGFGPI
                     TTGAIGIQYDNRDFGVTGEDAAYLHPTKTGSFAAYVFEEAAVTDQFKLQGAARIEWTD
                     LKGNTDAAGDLKRSFAPISYSLGAVYKPVEDTSFFTNFSETARAPAPTELFAQGAHDA
                     PGTFETGDPDIGLEKAFSMEGGVKHQDKDGNIASATVYRTRYNGFIDGFLTGNTCDED
                     GNCGNPGPGDFKQLFYVQKDATFWGFEGQVHWHVLDAGTGRVGIDLQTDYVRATLQGG
                     TNVPRIPPLRYGGGLFFESTLVELRLSVLRADSQDKTDAHETPTPGFTNVDASAVFHV
                     YRGQEGDFDVALVATNLTDAVERNAVSFTKDHVLQPGRAFRIMLHYMH"
     gene            complement(3335..4189)
                     /locus_tag="VGM72_11800"
     CDS             complement(3335..4189)
                     /locus_tag="VGM72_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265994.1"
                     /translation="MSNPGSAGLFAFVLCAAGAALAADGAKPGVPIFATTPNTGWVLD
                     HSFGVDDLLAPPGGGPGPVSFDKAHPYIPNNFGPQSTYRVADLSNPILQDWLKPSMKK
                     ANDAVLAGKVPYRARERCWPVGVPGFDAYSLVEPFYFYQTPKEVVVINQGGPEIRHIY
                     MNVPHSKTVKSSWYGESVGHYENGDTLVIDTIGQNDKSFTDNYRTPHTTQIHVIERWK
                     LAADAKTVDVSVYVEDPGAFTTPWTGFQRWRRVEEAPILQVPCNENNDDHFDQDLVPL
                     AQAAKPDF"
     gene            complement(4208..5113)
                     /locus_tag="VGM72_11805"
     CDS             complement(4208..5113)
                     /locus_tag="VGM72_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265995.1"
                     /translation="MRVLAARRIAGIAAACIMATFSVWPTMGANSAKAIPDFSGVWSH
                     PFLTGFEPPASGPGPVTNRSRRPDGVANFQTLVGDYTNPILKPRAADAVKAHGDISLA
                     GGGYPTPSNQCWPGGVPYVFWDFLMQMFQEPDHITMVYRQGNEVRRVRMNQPHPAHVT
                     PSWYGDSVGHYEGDTLVIDTVGIKKGPFAMLDMYGTPFSEKLHVTERYRLVDYEDAKD
                     AIDRNAKENSHAGLGWDFDPNYRGKVLQMHFTVEDDGVFTTPWTATITYRPSIDPWTE
                     LICAENPREFNNTDSHIPTAGKPDF"
BASE COUNT         1081 a         1632 c         1697 g         1112 t
ORIGIN      
        1 ggccgcgctc aaagacgcgc gcgaagcgat cggcgcattc acggtgcgct ttgagagcgc
       61 ctacacggag gagatgagga ggaagctggg ccttttgcaa tcgcgcgccg gcgatctcgc
      121 tttggcgcag gatctgctgg aacgcatggc gcgcaacagc gccgatttca cgctgacctt
      181 ccggcggttg tgcgatgcgg cagccgatcc cgcacgcggc gcgccggtgc gggctctgtt
      241 tgccgatcct gtcgcgttcg acgaatgggc ggtgacatgg cggcaacgtc tggccgaaga
      301 cggggggctg gaccgcgaat gttcagccgc gatgcgcgcg gcgaatcctc ttttcatccc
      361 gcgcaatcat cgggtggagc aggcaatctc cgcggctgtg aacgataacg atttcgtacc
      421 ctttgaaatg cttctgacgg tgctctcctc gccttacgcc gatcagccgg gtcatgaaat
      481 ctatgccgaa ccgccgcggc ccgatgagat cgtgcagcaa acattctgcg ggacgtgaga
      541 caagaagccg ccggacgctg tgggtggaaa ctctcttcca tttgacctaa tctggtcgaa
      601 gtggagagcc gcaggggatg atccagaacc gccgcatgag acgcatggaa gtgcgcgagc
      661 gcgcaagcgc cacgattatg cgccgcccgt cactgccgcg tgcggccttc gttttcctag
      721 ctctcgctgc gttggcgatt cagcttttcg tcgtccaaac ccatattcac attccccaag
      781 cgcaaggccg ctcagaatcg gtcagcatcg tgacgcttgc caaaaccctc atcgcggggc
      841 cgcaagacca aggcgctggc caacaggcaa atctgccgcg cgacaaatat ccgatcaatg
      901 aagacccctc gaattgcccg ctgtgtcagg aattggtgca ttccggtcaa ttcgtgcaga
      961 gcgtggctgt tctttcgcat atctcgttct gggtcagcgt ccatttcatt ccgctcagcg
     1021 aggcgctgcc caggctgttc attgtcagcc atagctggca aggccgcgcg cctccccaac
     1081 actgactccg cacattgatc ggctgatggg ctagggggct ttcacgccct cgcgtccatc
     1141 tcacgcgaat ccggcttttt cgcagtttca gatgcgggcc ggcatcgaac caccgtattt
     1201 tcgtggagtt ggtatcatga gatcgcaggt cttttccgcg gccctgacgg gcgcgagtct
     1261 ttttgttttg cagcccgctt tcgccgggca agcccccaca accaatcccg atgccgttcc
     1321 cgaacaggtc gtcatcacgg cctctccgtt cggtgaaaac gccatcgacc aatccgccag
     1381 cgtgtcgcag gtgacgcgcg agcaattgct gaattccggc ggcatcggcc tcggcgacgt
     1441 gttgaaggat gttcccggtg tcacgtcttc gggtttcagc gccggcacca gccggcccat
     1501 cattcgcggg ctgggtgcca cgcgcgtgcg cgtgacggag aacggcctgg gcagccatga
     1561 tgtggccgat gtcggcgatg atcatgcggt tcccatcgat ccgctggcct ccctcgatgt
     1621 cgaggtcctg cgcggcccgg ccaccctgcg ctatggcagc caggcgatcg gcggtgtcgt
     1681 caatgccatc aacaatcgca ttccgctgga tgtcggcgag ggcagcaatg tcgaaagctt
     1741 tgccgggatg tcgagcggca atcttgaacg gctcggcggt ggtctggccg attatcgcga
     1801 gggcaattgg gcgttccatg ccgatggggt cattcgcggc gcggacgatt acgatacgcc
     1861 gcagggcacc cagatcaaca ccttcgcatt cgggcggggt tacgcgctgg gcggcgccta
     1921 tattggcgat ggcggcacat ccggcgggct cggctataat cgttatatcg cccattatgg
     1981 catcgccagc gagcccggcg atccggaacg caagcacatc gatctcgacc agaagaatta
     2041 caacagcgcg atgcgcttca tggcgccgct cccggggatt accagcctga atgtgacagg
     2101 cggctattcc aattatcgcc acgacgaggt gacggatgcg gatggcatct ccgctctctt
     2161 caccaacaag gaatgggagg ggcgcgccga agcggtccac gagggctttg gcccgatcac
     2221 cacaggggct atcggcattc aatacgacaa tcgcgatttc ggcgtcaccg gcgaggatgc
     2281 cgcgtatctt cacccgacca agaccggcag cttcgccgcc tatgttttcg aagaagcggc
     2341 agtgacggat cagttcaagc tccagggtgc ggcgcgcatc gaatggaccg atttgaaagg
     2401 caataccgac gccgcgggcg atctcaagcg gagctttgcc cccatcagct actcgctcgg
     2461 tgcggtctac aagcccgtgg aagacacgag cttttttacc aatttctcgg agacggcgcg
     2521 cgcccccgca ccgaccgaac tgtttgccca aggcgcgcac gatgcgccgg gaacattcga
     2581 aaccggcgat cccgatatcg gtttggaaaa agccttttca atggaaggcg gcgtcaaaca
     2641 tcaggataag gacggcaaca tcgcgtccgc cacggtgtat cgcacgcgat acaatggttt
     2701 catcgatgga ttcctcaccg gcaacacctg cgacgaggat ggaaattgcg gcaatcccgg
     2761 tccgggcgat ttcaagcaac tcttctatgt ccaaaaggat gccaccttct ggggattcga
     2821 gggccaggtg cattggcatg tcctggatgc cggaaccggg cgtgtgggca tcgatctgca
     2881 aaccgactat gtgcgggcga ccttacaggg cggtacgaat gttccgcgta ttccgccgct
     2941 gcgctatggc ggaggcctgt tcttcgaaag cacgctggtc gaactgaggc tgagcgtgct
     3001 gcgggccgac tcccaggaca aaaccgacgc acacgagact cccacgccgg gattcaccaa
     3061 tgtcgacgca tcggcggtgt ttcacgtcta tcgcggacag gaaggggatt ttgacgtggc
     3121 gctggtggca acaaatctga ccgatgcggt cgagcgcaac gcggtttctt tcaccaagga
     3181 tcatgtgctg cagcccggcc gtgccttccg catcatgctg cattacatgc actgaccgca
     3241 ttttggaggg aaatggagca ttctcttgtg tccgccgggt tcccgctttt gcggggcatg
     3301 acccggcgga gacggggccg tgcgattctg cggctcagaa atcgggcttc gcggcttgtg
     3361 ccagcgggac caggtcctgg tcgaaatgat cgtcattgtt ctcgttgcag ggcacttgaa
     3421 ggatcggcgc ttcctcgacg cgtcgccagc gttggaaacc ggtccagggc gtggtgaacg
     3481 cgccgggatc ttcgacatag accgacacgt cgaccgtctt ggcatcagcg gcgagcttcc
     3541 agcgttcgat cacatggatc tgggtcgtgt gcggggtgcg gtaattgtcg gtgaagctct
     3601 tgtcgttctg gccgatggta tcgatcacca gggtatcgcc gttttcgtaa tggccgacag
     3661 attctccgta ccaggaggat ttcacagtct tagaatgcgg cacattcata tagatgtgac
     3721 gaatttccgg tccgccctgg ttgatgacga cgacctcttt tggtgtctga taaaaataga
     3781 acggttcgac cagcgagtaa gcatcgaagc ccggcacgcc gaccggccag cagcgttcgc
     3841 gcgcgcggta cggcactttg cccgccagca ccgcgtcatt ggcctttttc atcgacggtt
     3901 tcagccagtc ctgcagaatc ggattggaaa gatcggcgac gcgataggtc gactgcggcc
     3961 cgaaattgtt gggtatatag gggtgggcct tgtcgaagga gacgggaccc ggtccgccac
     4021 ccggcggcgc aaggagatcg tccacgccga aggaatgatc gagtacccag ccggtgttcg
     4081 gcgtggtggc gaaaatcggg acacctggct tcgcgccatc tgccgccagc gccgcgcccg
     4141 ctgcgcacaa cacgaaagcg aataggcctg ccgaaccggg attggacatc tgcgtttcct
     4201 cgatgaatca aaagtcgggc ttgccggcgg tgggaatgtg ggaatccgta ttgttgaact
     4261 cgcgcggatt ttcggcgcag atgagttccg tccacggatc tatggacgga cggtaggtaa
     4321 tggtcgccgt ccacggcgtt gtgaaaacgc cgtcatcctc caccgtgaaa tgcatctgca
     4381 gcaccttgcc gcgataattg ggatcgaaat cccaaccgag acccgcgtgg gaattttcct
     4441 tggcgttgcg gtcgatcgcg tctttggcat cttcataatc caccaggcga tagcgctcgg
     4501 tcacatgcag tttctcgctg aagggcgtgc cgtacatgtc gagcatggca aaaggcccct
     4561 ttttgattcc cacggtatcg atcaccaggg tgtcgccttc atagtgaccg acggaatcgc
     4621 cgtaccagga gggcgtcaca tgcgcaggat gcggctggtt catgcgcaca cggcgaactt
     4681 cattcccctg gcgatagacc atcgtgatgt gatcgggttc ctggaacatc tgcatcagga
     4741 aatcccagaa cacgtaaggc acgccccccg gccagcattg gttgcttggt gtcggatagc
     4801 cgccgccggc gagcgagata tcgccatggg ccttcaccgc gtcggctgcc cgaggcttca
     4861 ggatgggatt ggtgtaatcg ccaaccaatg tttggaaatt ggccacgccg tcgggccggc
     4921 gtgaccggtt ggtcacaggg ccgggccccg atgcgggcgg ctcgaacccg gtgaggaagg
     4981 gatgcgacca gacgccggaa aaatccggga tcgcttttgc gctgttcgcg cccatcgtcg
     5041 gccagacgga aaaggttgcc atgatgcaag ccgccgcgat accggcaatc ctgcgtgctg
     5101 cgagaactct catattcacc ctctggctgc gcatgtgaat tctaccgcac ccagccgcag
     5161 caaagaaact ggatgacgat gccgaagggg cgctgaattg tctggcgcga caggcttacg
     5221 ggccgcgctt ggtcaaatgc gacaccggaa atatagccga tgacgacgac gcagcgacga
     5281 tgccgaagac gaaacgccgc catggaacga aatcggcgag ggcctgccta tgggaacgct
     5341 ctgccgccat ggtttcattt ttccccgctg ttccagtcca aagataggcg ttctccggcg
     5401 caaaattgca agcctagggt ctggactcac aacttgatcc agacgagcgc gccgacgatg
     5461 gcagcggcgg caaggtaatt tctggcgagt ttgtcatacc gggttgcaat gcgcctccaa
     5521 tc
//
LOCUS       DASYEG010000279         8473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2280867, whole genome shotgun sequence.
ACCESSION   DASYEG010000279 DASYEG010000000
VERSION     DASYEG010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 8473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8473
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2280867"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..626
                     /locus_tag="VGM72_11810"
     CDS             177..626
                     /locus_tag="VGM72_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265996.1"
                     /translation="MRAILPILIMTLSLSQAGLAAPASAPRDMLIVPGDRAGAVALGM
                     TPAELTASVGTPADVQHQGPDTIYSWGDVTAEISDKSPNVDLISVNDPRYETADHVRV
                     GLAVFAVMAVLGDPVKITNALGIQDLEYDGMTIVVRNNLVAQIRVRK"
     gene            640..2346
                     /locus_tag="VGM72_11815"
     CDS             640..2346
                     /locus_tag="VGM72_11815"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY4265997.1"
                     /translation="MSAEATTAASGTLSVKDTIRHRRLSALDGIALVLSLTLAVPIAS
                     VCLSLFSGQSAVFVHLAETVLAEYAANTLILAALVVFGVLIVGVPAAWLTAACEFPGR
                     RWLEAALILPLAAPAYVLAYAYAGFLSAYGPVQEMLRNATGWDTGDYWFPDIRSLPGA
                     ALMLVLTLYPYVYLLARARFLNESASALEAARLLGRGPWSSFFAVSLPLARPALVAGA
                     TLAAMNTFADYGTVSYFGVPVFTTGIYSAWFSFSDQTAARELAAVLVLFVALALLAER
                     GLRGRARYHETGRRDRRPARYRLTHGKSAAAIVACVVPPLLGFVVPALVLGELLIESG
                     GPTRDFSRHLFNTLILAASAGAIVTLGALVLAFARKERPQGIAGRAAAFAGLGYAMPG
                     AVIAVGVLVPFAAFDNGVANFLQRAFGVSSGLILTGGIAALLFAYTALYLAVALQSVS
                     AGLERVTPSIGAAAKLLARGPWDVLRRVYLPLIAPSVLTAALLVFVDVMKELPATLML
                     RPFNFDTLAIAANNYASDERLSWAAAPSLAIVAAGIIPCIVLIRTITSSTKGRDVPPP
                     LP"
     gene            2469..2972
                     /locus_tag="VGM72_11820"
     CDS             2469..2972
                     /locus_tag="VGM72_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265998.1"
                     /translation="MRLWTMALACLMALGFARIAAAADAATSDGVPADWIEVAAGPMF
                     TVKAPPGTTFERIRTGDAFAGTFHGPGFDLPVEFGYHRDAVKLPGGAKDTTESKLVID
                     EKPGAITKAATADPAHPLFVGLYVPAVENDLLGPLSLVVTAALTKPEDAATVERIYRS
                     VKFGYKN"
     gene            3072..3233
                     /locus_tag="VGM72_11825"
     CDS             3072..3233
                     /locus_tag="VGM72_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4265999.1"
                     /translation="MSVSAIGAATPPSPVPAPSAKTENAKPPVTAEPQEAKPAHRHKH
                     AAHKVDVSA"
     gene            complement(3236..4255)
                     /locus_tag="VGM72_11830"
     CDS             complement(3236..4255)
                     /locus_tag="VGM72_11830"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY4266000.1"
                     /translation="MRFVPVFVFVLGLAALAGPANAVNALNIYSARHYDSDELLYDGF
                     QKVTGIPVNVIEGDGPELLARMKAEGKNSPADVFLTVDAGNLWLAEHENLFQPAQSKI
                     LDARIPASLKSPKNLWFGFSTRARLIFVNAKRVDPKLVQTYDSLADPRLKGKICMRSS
                     SAVYNLSLLGAMIAHMGPAKAEQWVKGIVANFARPPQGADTTLLQSVAAGECAVTIAN
                     HYYFMRLVNSKVKSERDAAKKLTPIFPDQDGFGTHVNVSGGGVMASAPHKAIAVKFLE
                     FMASDAAQNIIAGANYEFPAVPTVAITPELKTLGRFKTDPLNVSFYGVNQAQAQAIFD
                     RAGWR"
     gene            4437..6362
                     /locus_tag="VGM72_11835"
     CDS             4437..6362
                     /locus_tag="VGM72_11835"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEY4266001.1"
                     /translation="MSRTTRIELIVLAVVLVVLAAPFLIPLNMYRGRMEAAASNALSR
                     EVHIKGPLHLTFYPDLGVSLSDVSIGNLKGARNAQMITAEKLVVGAKLMPLFSGELQV
                     TELTLEKPVIHLETVKDAAPNWSFGKAPDSGKPADAAALNRIGFAHLNINNGAITYYD
                     AASGKSAALNEVSLSLNMPDAARPTLSLPLTLAGSVTYNGEALKIDGRLDNFGNLLNG
                     RTTGARISIGSNVINAEFTGQLGTASNLTGSLKLGAHSVRSLAAWLGNPMPPGNGFGL
                     LALEGHFTARDGVYALSHAHLAFDQMNLNGDINLDTNPKVMTLKGQVSIDRLNVNPYL
                     APGASDDTVKAAKAKAASPDAPMSLGGLRAVNADLTLIVGELDLPDLKLEQTIIKTSL
                     QDGLLQADMAKVSAYGGTGKASLTVDARNAIPQLHSTLDVTDIKVQPFLAQMMHFDRI
                     SGKGSVRYDVAATGDTAKAMLANLDGKGDIHFTEGAISGADLVAISRVLQSVVNGDAL
                     GAAIGESASTPFGKMGANFTIAHGIMQTKDFQLVNPTVEMNGRGNVDLNARTLEFHFE
                     PKAVKGIPGLKLVDIGVPFYVKGPWDKPGYGPDVRGLAKTIVEKLENGATSPLDVLTQ
                     PGLSLKSLLGTGRTSNK"
     gene            6373..7467
                     /locus_tag="VGM72_11840"
     CDS             6373..7467
                     /locus_tag="VGM72_11840"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY4266002.1"
                     /translation="MRLPRWLAATVLAVILLGAGFYWVTLPGPSITVVSWGDAYGRAQ
                     TLALFHPYTDKTHVDVNIANYGGGLKEISAQVASGDVQWDVVDMEVEDAAAACRQGLL
                     ENLEGIELPAGANGIGAQRDFVPGALGPCWVGSVVYSQVIGIDTMQFGDSKPETAADF
                     FDLARFPGPRGLRDAGPKYNLELALLADGVKFWQVYGVLGTEAGVDRAFAKLDAIKSS
                     IKWWRRPAEPADMLARGEVAMTTALNARIFSIDPEPKIRTIWDGQLYQLDVFAIPKGD
                     PHKARALDFIRFATGSAPLAEEARLLPYGPARTSAIPLVGLNPDTDADMRPHLPTTPR
                     NFVGALAVDPDWWAKHGASLEARWAAWRKN"
     gene            7487..>8473
                     /gene="oppB"
                     /locus_tag="VGM72_11845"
     CDS             7487..>8473
                     /gene="oppB"
                     /locus_tag="VGM72_11845"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF007008.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HEY4266003.1"
                     /translation="MWLDKVETLQRAASWSLILPMLSYAIRRSLGAIPTLFIIITLSF
                     FMMRLAPGGPFDSERVLPPDIARNIAAAYDLDKPVYQQYLIYLGRLLHGDLGPSYKIR
                     DFTVSQLIASGFPVSMKLGLLAITLAFAAGTTLGIAAALRQNRPADYSVMTLAMIGIT
                     IPAFVTAPLLQLVFGIYGIHLFGLDFTLPVGGWNGGAPANIILPVIVLALGPIAVIAR
                     ITRGSMVEVLRSNFIRTARAKGLPTWRIITHHALHAGLLPLVSYLGPAIADIVSGSLV
                     VEQIFGLPGIGRYFVQAALNRDYTLVLGVTIFYATLIIVLNLVADLLYGVLDP"
BASE COUNT         1505 a         2759 c         2540 g         1669 t
ORIGIN      
        1 tgtggagccg gtcatgggtc gttcgtggca gtttcttctg acggtggcca ttgtcgcggc
       61 ctgggcgagt tatgcgtatt attcctcgga ttattacgct gagcgctctt cgggcataaa
      121 acctgcggcg gcagccacag actgagccaa cagcggaaaa aaaacgcgag ccaagcatgc
      181 gtgccattct gccgattttg atcatgacgc tttcgctgag ccaggccggc ttggccgcgc
      241 cagccagcgc gccccgtgac atgctgatcg tgccgggaga ccgggccggc gccgtcgccc
      301 tcggcatgac cccggccgag cttacggcct ccgtgggcac gcctgccgat gtgcagcatc
      361 agggccccga cacgatctat tcctggggcg atgtgaccgc cgagatcagc gacaaatcgc
      421 cgaatgtcga tctcatcagc gtcaacgacc cgcgctacga aaccgccgac cacgtccgcg
      481 tcggccttgc cgtgttcgcc gtaatggcgg tgctgggaga cccggtgaag ataacgaacg
      541 cactgggaat ccaggacctc gaatatgacg gcatgacgat cgtcgtgcgc aacaatctgg
      601 tcgcccagat ccgcgtgcgg aaatgatcgt ggcgcacgca tgagtgctga ggcaaccaca
      661 gccgcgtccg ggaccctttc cgtcaaagac acaatacgcc accgtcgtct ttcagcgctc
      721 gatgggatcg cgctcgtgct gtcgctgacg ctggcggttc ccatcgcctc ggtgtgtctc
      781 agcctgttct ccggccaaag cgcggtgttc gtgcatctgg ccgaaaccgt gcttgccgaa
      841 tatgccgcga acacgctgat cctggccgcg ctggtcgtct tcggcgtcct catcgtcggg
      901 gtgcccgcgg catggctcac cgcggcgtgc gaatttcccg gccggcgctg gcttgaagcc
      961 gccctcatcc tgccgctcgc cgcccccgct tacgtgctcg cctatgccta tgcgggattt
     1021 ttgtccgcct acggaccggt tcaggagatg ttgcgcaacg ccacggggtg ggacaccggc
     1081 gattattggt ttccggacat acggtcgctc ccgggcgcgg ctttgatgct ggtcctgacg
     1141 ctctatccct atgtctatct tctggcgcgc gcccgcttcc tgaacgaatc ggcaagcgcg
     1201 ctcgaagccg cacggctgtt gggccgcggc ccctggtcgt ccttcttcgc ggtttcgctg
     1261 cccttggcgc gcccggcgct ggtggcgggc gcgacgctcg ccgccatgaa cacattcgcc
     1321 gattacggca cggtctccta tttcggcgtg ccggtgttca ccaccggcat ctacagcgcg
     1381 tggttttcct tctccgacca gaccgcggcg cgcgagctgg ccgccgtgct ggttcttttc
     1441 gtcgcgttgg cgcttctcgc ggagcgcggt ttgcgtggac gggcgcgcta tcatgaaacc
     1501 ggcaggcgcg accggcggcc cgcgcgctat cggctcaccc acggaaaatc cgccgccgcg
     1561 attgtggcct gcgttgtgcc gccgcttctg ggattcgtgg tcccggcttt ggtgctcggc
     1621 gaattgctga tcgaatcggg cggaccgaca cgcgatttct cgcgtcatct cttcaacacg
     1681 ctcatactgg cggcctcagc cggcgcgatc gtgaccttgg gcgccctggt gctggccttc
     1741 gcgcgcaagg aacggccgca gggaatcgca ggccgcgcgg ccgcatttgc gggcctcggc
     1801 tatgcgatgc ccggcgcggt gatcgccgtc ggcgtgctgg tgcctttcgc cgcgttcgac
     1861 aatggcgtgg cgaatttcct gcagcgcgcg ttcggcgtgt catccggcct catcctgacc
     1921 ggaggcatcg ccgcattgct gttcgcctat acggcgctct atctcgccgt ggcgctgcaa
     1981 agcgtaagcg ccggacttga acgggtgacg ccctccatcg gcgcggcggc aaaactgctc
     2041 gcgcgcgggc cctgggacgt gctgcgccgg gtctatctgc cgctgatcgc gccttcggtc
     2101 ctgacggcgg ccctcctcgt cttcgtggac gtgatgaagg aattgcccgc gacgctgatg
     2161 ctgaggccgt tcaatttcga cacgctcgcc atcgcggcca acaattatgc ttccgacgaa
     2221 cggctctcct gggcggcggc gccgtcgctc gccatcgtgg cggcggggat cattccctgc
     2281 atcgtgctga tccgtaccat cacatcctcc acgaagggac gtgatgttcc cccgccactg
     2341 ccctgacgcc atcgcggccc gcgcctctcg cttaagctcc cttcgacaag cgcaaggtga
     2401 gggaataaac tgcacgcccg cgctgaccgc tgttgatgca tgagcatctc gaaacgatgg
     2461 agagatcgat gcggctctgg acgatggctc ttgcctgttt gatggcgctc ggatttgcac
     2521 gcattgcggc tgcggctgac gcggcaacct ccgacggcgt gcccgccgat tggatcgagg
     2581 tcgcggcggg accgatgttc accgtgaaag cgccaccggg cacgacgttc gagcgcatcc
     2641 gcacgggcga tgcctttgcc ggcacgttcc acgggccggg cttcgatctg ccggtcgagt
     2701 tcggctatca ccgcgacgcc gtgaaattgc ccggcggcgc gaaggacacc accgaatcca
     2761 agctcgtcat cgatgagaaa cccggcgcga tcaccaaagc cgcgaccgcc gatcccgcgc
     2821 atccgctgtt tgtcggcctc tatgtgcctg cggtggagaa cgacctgctc ggtcctttaa
     2881 gtctcgtggt cacggccgcg ctaaccaaac ccgaagacgc ggcgaccgtc gaacgcatct
     2941 atcggtcggt caaattcggc tacaaaaact aaccctattt tcgttctggg gccggttaac
     3001 cacccttctg aaccttgcct tcacatctcg cgcccactct cccgcgtgca acgcagaaaa
     3061 tcgggtgcaa aatgtccgta tcggccatcg gcgcagcaac gcctccgtct ccggtgcccg
     3121 cgccatcggc aaagaccgaa aacgcgaaac cgccggtgac ggcggagccg caagaggcga
     3181 agcccgcgca tcgccacaaa cacgcggccc acaaggtcga cgtctcggcg tagcgtcagc
     3241 gccagccggc gcggtcgaag atcgcctgcg cctgcgcctg attcacgccg tagaacgata
     3301 cgttcagcgg atcggtcttg aaccggccca gcgtcttcag ttcgggcgta atcgcaaccg
     3361 tcggcaccgc gggaaattcg taattggccc ccgcgatgat gttctgcgcc gcatcgctcg
     3421 ccatgaattc caggaatttg acggcgatcg ccttgtgtgg cgccgaggcc atcacgccgc
     3481 cgcccgatac gttcacatgg gtgccgaaac cgtcctgatc cgggaagatc ggggtgagtt
     3541 tcttggcggc gtcgcgctcg cttttcacct tcgaattcac cagccgcatg aaataataat
     3601 gattggcgat ggtgacggcg cattcgcctg ccgccacact ctgcaacagc gtggtatcgg
     3661 ccccttgcgg cggcctcgcg aaattcgcca cgataccctt gacccattgt tcggccttgg
     3721 ctggacccat atgcgcgatc atcgcgccca gcagcgacag attgtagacc gccgaactcg
     3781 accgcatgca gatcttgccc ttcagccgcg gatcggcaag cgaatcatag gtctgcacca
     3841 gtttgggatc gacgcgtttg gcattcacga agatcagccg cgcgcgcgtg gagaaaccga
     3901 accacaaatt tttcgggctc ttgagcgagg ccgggatgcg cgcatccaga atcttggact
     3961 gagccggctg gaacaagttc tcatgttccg ccagccacag attgcccgcg tccacggtca
     4021 ggaacacgtc ggcgggggaa ttctttcctt cggccttcat gcgggcgaga agttcgggcc
     4081 catcgccttc gatgacgttg accggaattc ccgtcacctt ctgaaaaccg tcgtaaagca
     4141 gctcgtcgga atcataatgg cgcgccgaat agatgttcag cgcgttgacg gcatttgcgg
     4201 ggcccgccaa tgcggcaagg ccgagcacga aaacgaagac gggaacaaaa cgcatgggct
     4261 ggcttctttt atgcgaatga ttatcataag cgatatgatc cgccgcgcaa tcgtaaaaac
     4321 cgcgtcgcgt gacgcatttt cgccgtcatg gcgaaacaga ctggaagaac cggcacattc
     4381 attgggccat tcttgatcca tttttcgcac gttcccgtga ccgcttaagg aacttcatga
     4441 gccgcacgac ccgcatcgag ttgattgtcc ttgccgtcgt gctggttgtt ctggcggcgc
     4501 cctttctgat tccgctcaat atgtatcgcg gccggatgga agcagccgcc agcaacgctc
     4561 tgtcgcgtga ggtccatatc aaggggccgc tgcacctgac attctatccc gatctcggcg
     4621 tttcgctgtc cgacgtctcc attggcaatc tcaagggcgc gcgcaatgcg cagatgatca
     4681 cggcggaaaa actggtcgtg ggcgccaagc tcatgccgct cttttccggc gagcttcaag
     4741 tcaccgagct tactttggaa aagccggtta tccatctcga gaccgtgaag gatgccgcgc
     4801 ccaattggtc cttcggcaag gcgcccgatt ccggcaagcc cgccgatgcg gcagcgctca
     4861 atcgcatcgg atttgcgcat ctgaacatca ataacggcgc gatcacctac tacgacgcgg
     4921 caagcggcaa gagcgcggcg ctgaacgagg tttccctctc gctcaacatg cccgacgcgg
     4981 cgcggccgac gctgtcgctg ccgctgacgc tggcggggtc ggtgacgtat aatggcgagg
     5041 cgctgaagat cgacggccgc ctcgacaatt tcggcaatct cttgaacggc cgcaccacgg
     5101 gtgcgcgtat ctccatcggc tcgaacgtca tcaacgccga attcaccggc cagctcggta
     5161 ccgcgagcaa tctcaccggc tccttgaagc tgggcgcgca ttccgtgcgc agtctggcag
     5221 cctggctggg caatccgatg ccgccgggca atggcttcgg cctgctggcg ctggaaggtc
     5281 atttcactgc gagagacggc gtctacgcgc tcagccacgc tcatcttgcc ttcgatcaga
     5341 tgaatctgaa cggcgatatc aatctcgata ccaatcccaa ggtgatgaca ctgaagggcc
     5401 aggtttccat cgaccgtctg aacgtcaatc cctacctcgc gccgggcgct agtgacgata
     5461 cggtcaaagc cgccaaggca aaagcggcca gtcccgatgc gccgatgtcg ctcggcggat
     5521 tgagggcggt gaatgccgat ctcaccttga tcgtgggcga actcgacctg cccgatctca
     5581 agctcgaaca gaccattatc aaaaccagcc tccaggatgg cctgctgcaa gccgacatgg
     5641 cgaaagtcag cgcctatggc ggcaccggca aagccagcct caccgtggac gcgcgcaacg
     5701 cgatcccgca gctccacagc acgctcgacg tgactgacat caaagtgcag cccttccttg
     5761 cgcagatgat gcatttcgac aggatcagcg gcaaaggaag cgtgcgctac gatgtcgctg
     5821 ccaccggcga tacggccaag gcgatgctcg cgaacctgga cggcaaaggc gatatccatt
     5881 tcacggaggg cgccatttcc ggtgcggatt tggtggcgat ttcgcgggtg ctccaatcgg
     5941 tggtgaacgg cgatgcgttg ggcgcggcca ttggtgaaag cgccagcaca cctttcggaa
     6001 agatgggcgc aaatttcaca atcgcgcacg gaatcatgca aaccaaggat ttccaactgg
     6061 tgaatcccac cgtggagatg aacggccgcg gcaatgtcga cctcaacgcg cgcacgctcg
     6121 aattccattt cgagccgaaa gcggtcaagg gcataccggg actgaagctc gtggatatcg
     6181 gcgtcccctt ctatgtgaag ggtccgtggg acaagcccgg ttacggcccc gatgtgcgcg
     6241 gccttgccaa gaccatcgtc gagaaactcg aaaacggcgc cacctcgccg cttgacgtcc
     6301 tgacacagcc cggcttgtct ctgaaatccc ttttgggtac gggaaggacg tccaacaagt
     6361 agcgtaatcc ccatgagact tccgcgctgg cttgccgcga cggtcctggc cgtcatcctc
     6421 ctcggcgcgg gcttttactg ggttacgctg cctggccctt cgatcaccgt ggtgagctgg
     6481 ggcgacgcct atggccgggc gcaaaccctc gcgctgtttc atccgtatac agataagacc
     6541 catgtcgatg tgaacatcgc caattatggc ggcgggctga aggagatctc cgctcaggtc
     6601 gcgtcgggcg acgtgcaatg ggatgtggtc gacatggagg tcgaagacgc ggccgccgct
     6661 tgccgccaag gactgctcga aaatctggaa ggcatcgaac tgcccgcggg cgcgaacgga
     6721 atcggcgcgc agcgcgactt cgtgcccggc gcgctgggac cgtgctgggt cggcagcgtc
     6781 gtctacagtc aggtgatcgg catcgacacc atgcaatttg gagattccaa gcctgagacc
     6841 gccgccgatt tcttcgatct cgcacgcttt ccaggtccgc gcggacttag ggacgccggt
     6901 ccgaaataca atctcgagct ggcgcttctc gccgatggcg tgaagttctg gcaggtctat
     6961 ggcgtgcttg gcaccgaagc cggcgtcgat cgcgcgttcg ccaagctcga tgcgatcaaa
     7021 tccagcatca aatggtggcg ccgtccggcg gagccggccg acatgctcgc acgcggcgag
     7081 gtggcgatga ccaccgcgct gaacgcacgc attttcagca tcgacccgga gccgaaaatc
     7141 cggaccatct gggacggcca gctttaccag ctcgacgtgt tcgccattcc caagggcgat
     7201 ccgcacaagg cacgcgcgct ggatttcatc cgcttcgcga ccgggtccgc gccgctggcc
     7261 gaggaggcgc ggctgcttcc ctatggcccg gcccgtacgt ccgcgattcc ccttgtcggg
     7321 ctcaatcccg ataccgatgc ggatatgcgc ccccaccttc cgaccacgcc gcggaatttc
     7381 gtgggcgccc ttgccgtcga tcccgattgg tgggcgaagc acggcgcatc gctcgaagcg
     7441 cgctgggcgg catggcgcaa aaattgagcg ccgcattgac attggcgtgt ggctcgacaa
     7501 ggttgaaact ctccaaagag ccgcgtcctg gagcctcatc ctacccatgc tgtcctatgc
     7561 gataaggcga agcctgggcg cgatcccgac gcttttcatc atcatcaccc tttccttctt
     7621 catgatgcgc cttgcccccg gcggaccgtt cgattccgag cgggtgctgc cgcccgacat
     7681 cgcgcgcaac atcgcagccg cctacgacct ggataagccc gtctatcagc aatatctgat
     7741 ctatctgggg cggctgctgc atggcgatct cggcccctcc tacaaaattc gcgatttcac
     7801 cgtgtcgcag ctcatcgcga gcggctttcc cgtcagcatg aagctggggt tgctcgcgat
     7861 cacgctggcc ttcgcagccg gcaccacgct ggggatcgcc gccgccctgc gccagaatcg
     7921 cccggcggat tattcggtga tgacgctcgc catgatcggc atcacgattc cggctttcgt
     7981 caccgcgccg ctcctgcaac tcgtcttcgg catctatggc atccatctgt tcgggctcga
     8041 tttcacgctg ccggtcggcg gctggaatgg cggcgccccg gccaacatca tcctgccggt
     8101 gatcgtattg gcgctggggc ccatcgccgt catcgcgcgc atcacgcgcg gcagcatggt
     8161 cgaagtgctg cgctccaatt tcatccgcac ggcacgcgcc aagggccttc ccacctggcg
     8221 gatcatcacc catcacgcgc tgcacgcggg cctgttgccg ctggtgagct atctcggccc
     8281 ggccatcgcc gacatcgtga gcggttcgct cgtcgtggaa cagattttcg ggcttcccgg
     8341 catagggcgt tatttcgtgc aagctgccct caatcgcgat tacacgctgg tgctcggtgt
     8401 caccattttc tatgccacgc tcatcatcgt gctgaacctg gttgccgatc tgctctatgg
     8461 cgtgctcgat ccg
//
LOCUS       DASYEG010000280         2031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4457825, whole genome shotgun sequence.
ACCESSION   DASYEG010000280 DASYEG010000000
VERSION     DASYEG010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4457825"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VGM72_11850"
     CDS             complement(<1..769)
                     /locus_tag="VGM72_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY4266004.1"
                     /translation="MTRSYIVDDPAKQRFLLDREALVSDQVLNDEMAKIFTKCWIYVG
                     HASELKKPNDFVTRKVAGRPVIFNRDMKGDIHCFLNTCRHRGAIVCTEPAGSRRAFRC
                     LYHGWAYRNDGMLGNVPGAEAYRGEFDKSALGLRSPARFESYRDFWFLNLDADAPSLR
                     DYLGRATEYIDLVIDQSPSAQMEIVAGAQEYDVAANWKLMVENSVDDYHLPTTHSTWL
                     DYMANSGVTIARPTDGRVLPTHGIAVDLGNGHFTTDNI"
     gene            856..>2031
                     /locus_tag="VGM72_11855"
     CDS             856..>2031
                     /locus_tag="VGM72_11855"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY4266005.1"
                     /translation="MHDIVKDSHWHEPKAGENAGTGTFSRPKLPYDRFMETEGVPVYR
                     GIGARSVKDLPMAPWKRLGGRGTYIQLYGTEGLWGMYVVEVPGAGALNVERHLYEKIV
                     FVVEGRGSTEVWQEGETRRHVFEWQKGSLFTIPLNAFHRIVNAGSAPALLLCGTSAPN
                     VMNLMDSPEFVFDCPHAFRSRFSGAEDYFKPRDDIEPDPVRGLAMRRTNFIPDVITTE
                     LPLDNRRSPGYRRMEPMMAGNRFYQFIGQYETGRYSKAHKHISTAVLICLKGNGYTYT
                     WPENLGPSPWRDGNGDKVLRQDYEFGGIVSAAPMSGDWFHQHFGVAKEGLRVLAWHGP
                     NNHPALKAGRPGEALKDIWAVDLNKGGNAIPYHMEDPALRQEYDETLARSGLASRMEP
                     "
BASE COUNT          393 a          594 c          652 g          392 t
ORIGIN      
        1 tgatgttgtc ggtggtgaaa tggccgttgc ccagatcgac cgcgataccg tgagtcggca
       61 gcacgcgccc atccgtgggg cgcgcgatcg tcacgccgga attggccatg taatcgagcc
      121 aggtcgaatg ggtggtgggc aggtgataat cgtcgacgct gttctcgacc atcagcttcc
      181 aattggccgc gacgtcatat tcctgcgcgc ccgccacgat ctccatctgt gccgacggcg
      241 attggtcgat gacgagatcg atatactccg tggcgcggcc cagataatcg cgcaaggagg
      301 gcgcatcggc gtccagattg aggaaccaga aatcgcgata ggattcgaag cgcgcagggc
      361 tgcgcaatcc cagcgccgat ttgtcgaact ccccgcgata ggcttcggct cccggcacat
      421 tgccgagcat cccgtcattg cgataggccc agccatgata gaggcagcga aaggcgcgtc
      481 tggaacccgc cggttcggtg cagacaatgg cgccgcgatg gcggcaggtg ttgaggaagc
      541 aatggatgtc gcccttcata tcgcgattga agatcaccgg gcggccggcc accttgcgcg
      601 tcacgaaatc attgggcttc ttcagttccg aggcgtgacc gacatagatc cagcatttgg
      661 tgaagatctt cgccatctcg tcgttcagga cctgatccga caccagcgct tcgcggtcca
      721 gcaggaagcg ctgtttggcg ggatcatcga cgatgtagct gcgtgtcatg gacattcctc
      781 taacccgtgc gcgagagtag cgtccagccc taaccgatgc tatgggcgca aggcccgtcc
      841 cacggaaaga ccgcgatgca cgacattgtc aaagacagcc attggcacga acccaaagcg
      901 ggcgagaacg cgggcaccgg cacgttcagc cgcccgaagc tcccctatga ccgcttcatg
      961 gagaccgagg gcgtgcccgt ctatcgcggc atcggcgcgc gctcggtcaa ggatctgccc
     1021 atggcgccgt ggaaacggct gggtgggcgc ggcacctata tccagctcta cgggaccgag
     1081 ggcctgtggg gcatgtatgt ggtggaggtg cccggtgcgg gtgcgctgaa tgtcgagcgc
     1141 catctctacg agaagatcgt gttcgtggtg gagggccgcg gttcgaccga agtctggcaa
     1201 gaaggcgaaa ctaggcgcca tgtcttcgag tggcagaagg gctcgctgtt caccattccg
     1261 ctgaatgcgt ttcaccgcat cgtcaatgcg ggttctgcgc cggcgctgct gctctgcggc
     1321 acttcggcgc cgaacgtgat gaacctgatg gacagcccgg agttcgtgtt cgattgcccg
     1381 cacgcattcc gttcgcgctt ttcgggcgcg gaggattatt tcaagccgcg cgatgacatc
     1441 gaacccgatc cggtgcgcgg tcttgccatg cggcggacca atttcattcc cgatgtgatt
     1501 acgaccgaac tgccgctgga taatcgccgc tcgcccggct atcgccgcat ggagccgatg
     1561 atggcgggca atcgtttcta tcaattcatc ggccaatacg agaccgggcg ctattccaag
     1621 gcgcataagc atatttcgac cgcggtgctg atctgcctga agggcaacgg ttacacctac
     1681 acctggccgg aaaatctggg gccgtcgccg tggcgcgatg ggaatggcga taaggtgctg
     1741 cgtcaggatt acgaattcgg cggcatcgtc agtgcggcgc cgatgtcggg cgattggttt
     1801 catcagcatt tcggtgtggc aaaagaaggt ttgcgcgtgc tcgcctggca cgggcccaac
     1861 aatcatccgg cgttgaaggc gggccgtcct ggcgaagcgc tgaaggacat ctgggccgtc
     1921 gatctgaaca agggcggcaa cgcgatcccc tatcacatgg aagaccccgc gctgcgtcag
     1981 gaatatgacg agaccctggc gcgttcagga ctcgcctcgc gcatggagcc g
//
LOCUS       DASYEG010000281        11080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3587241, whole genome shotgun sequence.
ACCESSION   DASYEG010000281 DASYEG010000000
VERSION     DASYEG010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 11080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11080
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3587241"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /gene="gshB"
                     /locus_tag="VGM72_11860"
     CDS             complement(<1..761)
                     /gene="gshB"
                     /locus_tag="VGM72_11860"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEY4266006.1"
                     /translation="MNLTVAIQMDPIERIDIVGDSTFALALEAQARGHALLYYGPREL
                     SLRETRLVTRVRPLTVRDRRGDHFSLGESRGYELSDADVVLMRQDPPFDMAYITATHL
                     LERIHPATLVVNDPFHVRNAPEKLFVTNFRDVMPPTLISSDPVEIRAFRLQHKEIILK
                     PLYGNGGAGVFRLKPDDENLKALLEMFTQFYREPVIVQRYVPEVRKGDKRIILVDGVA
                     IGAINRVPAEGEARSNMHVGGRAEPVDLSGRDRVI"
     gene            complement(772..1659)
                     /locus_tag="VGM72_11865"
     CDS             complement(772..1659)
                     /locus_tag="VGM72_11865"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEY4266007.1"
                     /translation="MNTPGSTDSASAPVAPAEFLRRLGQAGDGPYDLASAGLMLAALD
                     RPEKKLAPFHAHLAELAEAVSAQSQFTWHAESAARALSSIIANHCGYEGEREHYDDPQ
                     NADLMAVIEKRRGLPVALGIIYIHAARASGMEACGLLSPGHFLLKVTVKGSEALIDPF
                     NGGALVESEQVDAPQFGALSFAVETRGGIEPGRDFPDPLSPVGDSDVLLRLQNNIKTR
                     ALKARDISRAIEILKRMTLIAPRRGILWLELAHLQESLGALSAARGAYETCLALARPG
                     ENISNEAAFALHALKRRLN"
     gene            complement(1656..2282)
                     /locus_tag="VGM72_11870"
     CDS             complement(1656..2282)
                     /locus_tag="VGM72_11870"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY4266008.1"
                     /translation="MNPHRYFLIRLAPLVLIPLLSGCVWVAATGTVAGVSAARQERTI
                     GHALDDTVIKATLDGRLAQQSPGLYLRTSTTVVEGRVLLAGRVDSPEIRLDATRIAWS
                     VDGVRKVDNDIEVSDISGWLDGPSDLIMRTRLATILLADKTIKDVNYTTDVVHGVVYL
                     MGVAQNKEELDRVVEHAQKLNGAKRVENYVVLKDDPIRTALPPSSAER"
     gene            complement(2310..2687)
                     /locus_tag="VGM72_11875"
     CDS             complement(2310..2687)
                     /locus_tag="VGM72_11875"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEY4266009.1"
                     /translation="MTRRPDRGKAAESHGRRSERFAALFLACKFYRVIGKRVKTRAGE
                     LDLVAMSPSGVICFIEVKARRLENDAVEAVTARQRQRIARAAALYLGTRPSLRHKGVR
                     FDAILVTPRHWPRHLKDAWRPDS"
     gene            complement(2671..3663)
                     /gene="rsmI"
                     /locus_tag="VGM72_11880"
     CDS             complement(2671..3663)
                     /gene="rsmI"
                     /locus_tag="VGM72_11880"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEY4266010.1"
                     /translation="MAPPTHSHADAGAPEESNRDPKRETGKGKSHPSGTGESWETSIA
                     PVLAPGLYITATPIGNAADITLRALTVLKHCDAIVAEDTRVTSRLLSLYGISRPLFVY
                     NDHNAASMRPKLLSKLRTGARLALVSDAGTPLISDPGHKLVREALEEELPVFPIPGAS
                     AVLAGLVCAGLATDRFFFAGFLPSKAGERRSRIAGLKAISATLVFYESAQRLGESLAD
                     MTEILGSRDAAVARELTKLHEEVRRGTLGELASHYAGPPPKGEIVLLVGPPAEADAPD
                     STRIDALLTEALGFMPVKSAASLIAEATGIGRRAVYARALALKGLNRADDDEEA"
     gene            3681..4994
                     /locus_tag="VGM72_11885"
     CDS             3681..4994
                     /locus_tag="VGM72_11885"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEY4266011.1"
                     /translation="MNVFACFSGDVPNPAGPLKAITRLRESRGKVCAIACTVAIGALL
                     AGCQTMKPVAAPPPPPPPPPMRMVMAPPPPPPSRMEQPNFYRLRNSPSGVVPARVALL
                     LPLSGGTAETRAVAEALEKAAEMAVFDSKNTDILLMPRDDGGTPAKAAAAATKAINDG
                     AEIIIGPLFAALVTAVTPVARANHVPVIALSSDRSVGGNGVYLLSFQPETEVNRIISY
                     AVRTGHSRFAAMIPKNAYGEKVEAAFRDSVTRAGANVADVEAFDERPQVVGPPAKAAA
                     QSGADAILIADGGAMLQAIGPALALNGATSRTMQFLGTGLWDDASIAREPVLANGRFA
                     APPPESFRSFAAHYREIYEAAPPRIATLSYDALSLVALLTRGRPYQRFTDAALTDPNG
                     FSGVDGIFRFRADGSAERGLAILQVSQGGFSVIDPAPKSFPQPGF"
     gene            complement(4991..6151)
                     /gene="hemW"
                     /locus_tag="VGM72_11890"
     CDS             complement(4991..6151)
                     /gene="hemW"
                     /locus_tag="VGM72_11890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEY4266012.1"
                     /translation="MNAFGIYVHWPFCAAKCPYCDFNSHVRERYDEMRWARAVASELR
                     AAAALQGSNRPEVTSIFFGGGTPSLMGGVAVGAVLDAIAQFWRVTPDAEVTLEANPNS
                     VEQSRFADYRAAGVNRVSIGVQALDETALKGLGRLHGVEEARRAIRLGLKIFPRVSFD
                     LIYARPEHDHAAWALELKEALSFGTEHLSLYQLTIEQGTAYATLARQGKLIVPDEDRA
                     AELYELTQEICDKAGLPAYEISNHAQRGAESRHNLTYWRYGDYVGTGPGAHGRLTLSE
                     RRLATIAERLPERWLEEVERRGSSFVMTDVNNNEAAQEHLLMSVRLTEGLDIASYRAR
                     WNVEPSPVRLEALQGAGLIALDEGRLKATPRGRLVLNGIIGKLAEALEPQFA"
     gene            complement(6148..6765)
                     /gene="rdgB"
                     /locus_tag="VGM72_11895"
     CDS             complement(6148..6765)
                     /gene="rdgB"
                     /locus_tag="VGM72_11895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEY4266013.1"
                     /translation="MIRLKSGDRLVVASHNPGKVKEIRALIAPFGIETVGAAELGLPE
                     PEETGTSFAQNAALKADAATRACGLPALSDDSGLCVAALDGAPGIYSARWAGPQKDFR
                     LAMRRVETELREKDTHDARAYFVCALALAAPGAVTQIFEGRVFGALAFPPRGDKGFGY
                     DPIFIPEGHRLTFGEMAPSAKHAMSHRARAFAKFVAGAVAPDTEQ"
     gene            complement(6762..7490)
                     /gene="rph"
                     /locus_tag="VGM72_11900"
     CDS             complement(6762..7490)
                     /gene="rph"
                     /locus_tag="VGM72_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEY4266014.1"
                     /translation="MGPSLRPSARRNDEMREVNLEPGVLRHAEGSCLVKFGHTHVLCT
                     ASLENQAPPFLRGSGRGWVTAEYGMLPRATSERTRREAATGKQTGRTQEIQRLIGRSL
                     RAVTALELLGERQIIIDCDVIQADGGTRTASITGGFVALYQCISFMAGTKLIARHAIK
                     DHVAAVSCGIYENTPVLDLDYAEDSTAGTDANFVLTGAGGIVEIQGTAEGVPFSEAEF
                     GQLLGLARKGIAELMDLQKQALKL"
     gene            7614..8711
                     /gene="hrcA"
                     /locus_tag="VGM72_11905"
     CDS             7614..8711
                     /gene="hrcA"
                     /locus_tag="VGM72_11905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEY4266015.1"
                     /translation="MEARTPKAMAGDFVASELGSRPREVFRHLVEAYLSSGEPVGSRT
                     LSQRLPLTLSPASIRNVMADLEGMGLLYAPHTSAGRVPTEKGLRLFVDGMLQIGDLTS
                     AERSSIEDRIKASGRPFEDVLTQAASLLSGLSRCAGLLVAAKQETALKHMEFVAIGPG
                     KALAILIGADGSVENRVIDTPLGLLPSALTEAGNYLSSKLQGRTLESAKAEILKEIEE
                     ERTELDVLTARIVAEGLATLSARAPREGADAAGDRILIVRGASNLLDDIEAQGDLARV
                     RTLFDDIERKNDLIGLLELARQGEGVHIFIGSENKLFSLSGSSIVAAPYADSKGKVVG
                     VIGVIGPTRLNYARIIPMVDYTARVVGRLLA"
     gene            8744..9343
                     /gene="grpE"
                     /locus_tag="VGM72_11910"
     CDS             8744..9343
                     /gene="grpE"
                     /locus_tag="VGM72_11910"
                     /inference="COORDINATES: protein motif:HMM:NF010739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY4266016.1"
                     /translation="MSAENDNQPLPEDIGQETLSEQEAVHGVINALRVEADAQKDRAL
                     RALAEVENVRKRLERERDEARIYAVTRFARDLLTVGDNLSRALAAVPADVRAQAGDSL
                     TAVLDGVEATERELKATLTRHGVKPIEAEGVRFDPHLHQAIAEVPAEGAEPGTVMHVV
                     QPGYTIAERLLRPAMVTVAKGAPRPNGGNGAGSQIDSKI"
     gene            9385..9534
                     /locus_tag="VGM72_11915"
     CDS             9385..9534
                     /locus_tag="VGM72_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266017.1"
                     /translation="MNPIIRLARPGYITGSAQGAFMVAVLEAVLGASLQYFKVRRAGR
                     VMRRA"
     gene            9531..10340
                     /gene="dapB"
                     /locus_tag="VGM72_11920"
     CDS             9531..10340
                     /gene="dapB"
                     /locus_tag="VGM72_11920"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEY4266018.1"
                     /translation="MSRIRIAVAGASGRMGRTLIRGLAENPDMTFAFALERPGHASLG
                     TDAGVLAGLPPNGIAVTGDSLAALAACDAVLDFTAPSSSVALSELAAQARIVHVIGTT
                     GFSDGELGRLKAAARHAVIVKSGNMSLGVNLLAALVREAARALPGYDVEILEMHHRMK
                     VDAPSGTALLLGEAAAQGRDIALKDRAVRVRDGQTGIRPEGAIGFASLRGGTIVGDHM
                     VVLAGPSERIELTHRAEDRMIFAKGALAAARWGQGKKPGLYAMSDVLGLSR"
     gene            10373..>11080
                     /locus_tag="VGM72_11925"
     CDS             10373..>11080
                     /locus_tag="VGM72_11925"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4266019.1"
                     /translation="MRLTIFILAALTVLAGGAASAATPEETSAACAADDPDVAILACS
                     ALIESGRESPADLSRDYNNRGGAFSAKGDYDHAILDLDEAVKLDAQNIGALINRGNSY
                     GGIRQYDRALEDYSAALKLDANDSRAYEARGGVYEKLDRPGDALADYDTAVLLDAHNI
                     DALISRGVLYRSQGEVEAGIADFTAAIALHPDFATAFDNRGFAYAIQQRYEHALADYD
                     EAVRLNPHAMVTLYHRGV"
BASE COUNT         2045 a         3533 c         3439 g         2063 t
ORIGIN      
        1 cagatgacgc ggtcgcggcc cgacaaatcc accggctccg cccggccccc gacatgcatg
       61 ttggaacgcg cctccccctc ggccggcacg cggttgatcg caccaatcgc cacgccatcg
      121 acaagaatga tgcgcttgtc gcctttgcgc acttctggaa cataacgctg cacgatcacc
      181 ggctcgcgat agaactgcgt gaacatttcc agcaatgctt tcaggttctc gtcatcgggc
      241 ttcagccgga agactcccgc gccgccattg ccgtacagcg gtttcaggat gatctccttg
      301 tgctgcaggc gaaaggcgcg gatctcaacc gggtctgagc tgattaaggt cggcggcatc
      361 acgtccctga aattggtgac aaacaatttt tccggcgcat tgcgcacatg gaagggatcg
      421 ttcaccacca gcgtcgccgg atggatacgt tccagcagat gcgtcgcggt gatgtaggcc
      481 atgtcgaagg gcggatcctg gcgcatcagc accacatcgg catccgacaa ttcgtagccg
      541 cgcgattccc ccaaactgaa atgatcgcca cgtcgatcgc gcaccgtcaa cggccggacg
      601 cgggtcacca atcgggtttc gcgcagagag agttcgcgcg ggccataata gagcagcgca
      661 tggccgcgcg cctgggcttc gagcgccagc gcgaaggtgg aatcgcccac gatatcgatt
      721 cgttcgatgg gatccatctg gatcgcgacg gtcaggttca tcgccatgct ttcaattgag
      781 ccgccgcttc agcgcatgga gcgcgaatgc cgcttcgttc gagatgttct cgccgggacg
      841 cgcgagcgca agacacgtct cataggcgcc gcgcgcagcc gacagcgcgc ccagcgattc
      901 ctgcaaatgc gccaattcca gccacaagat gccgcgcctc ggcgcgatca acgtcatgcg
      961 cttcaaaatc tcaatggcgc gggagatgtc ccgggcctta agcgcccgag tcttgatgtt
     1021 gttctgcagc cgcagaagca catcgctatc gcccacgggc gaaagcggat cggggaaatc
     1081 ccgcccgggc tctatgcccc cacgtgtctc cacggcgaaa gaaagtgcgc cgaattgcgg
     1141 agcgtccacc tgctcgctct cgaccagggc gccgccattg aacgggtcga tcaaggcttc
     1201 gctgcccttg accgtcacct tgagcagaaa atggccgggc gacagaagac cgcaggcctc
     1261 catgccgctg gcacgcgccg catggatgta gatgatgccg agtgccaccg gcagcccgcg
     1321 ccgtttttcg atgacggcca tcaggtcggc gttctgcgga tcgtcgtaat gctcgcgttc
     1381 gccttcatag ccgcaatgat tggcgatgat ggaggaaagc gcgcgcgcgg cgctttcggc
     1441 atgccacgtg aactgggatt gcgcgctcac tgcctcggcg agttcagcca gatgcgcgtg
     1501 aaacggtgca agcttcttct ccggacggtc gagagcggcc agcatcaaac cggcggatgc
     1561 gagatcatag ggtccatcgc ccgcttggcc gagccgccgc agaaattctg cgggcgcaac
     1621 cggcgcggac gccgaatctg tggatcccgg cgtgttcatc tctcggctga ggatggggga
     1681 agcgcggtgc ggatcggatc gtccttcagc acgacgtaat tctccacccg tttcgcgcca
     1741 ttgagctttt gcgcatgttc gaccacccgg tccagttcct ccttattctg cgcgacaccc
     1801 atcagataga ccacgccatg caccacatcg gtggtgtaat tcacatcttt gatggtcttg
     1861 tcggccaaga ggatggtcgc gagccgcgtg cgcatgatga ggtcggacgg cccgtcgagc
     1921 cagcccgaaa tatccgagac ctcgatatca ttatcgactt tgcgcacgcc atcgacactc
     1981 caggcgatgc gggtcgcatc cagccggatt tctggcgaat cgacgcgacc ggccagcagc
     2041 acgcgcccct caaccacggt cgtcgaggta cgcaggtaaa gaccgggcga ttgctgcgcc
     2101 aaacgtccgt cgagagtcgc cttgatcacg gtgtcgtcga gcgcatggcc gatggtgcgc
     2161 tcctgccgcg ccgcggaaac acctgcgacc gtgccggtcg ccgcaaccca gacgcatccg
     2221 gacaagagcg ggatcaatac caatggggcg agacggatta gaaaataccg atgcgggttc
     2281 atcgcaaact ctcctcaact cacgcgccgt cacgaatccg gccgccacgc atccttcaga
     2341 tgacgcggcc aatgacgcgg cgtgaccaat atcgcgtcga atcgcacgcc tttgtggcgt
     2401 aaactgggtc gtgtgcccaa gtaaagcgcc gccgcgcgcg cgatgcgttg acgctggcgg
     2461 gcggtcacgg cctcgaccgc gtcattttca agcctgcgcg ctttgacctc tatgaaacaa
     2521 atgacgcccg atggactcat ggcgaccaga tcgagttcgc cggcccgcgt cttcacgcgt
     2581 ttgccgatca cccgatagaa tttacaggca agaaacaggg ctgcaaaccg ttcgcttctt
     2641 cgcccatggc tctcggcggc tttgccgcgg tcaggcctcc tcgtcatcgt ccgccctgtt
     2701 gagacctttg agcgccagcg cacgggcata gacggcgcgc cgtccgatgc cggtggcctc
     2761 ggcaatcaac gatgcggcgg acttgaccgg catgaaccca agcgcctcag tcaacagagc
     2821 atcgatgcgg gtcgaatcgg gggcatcggc ttcggcgggc ggacccacga gcagcacgat
     2881 ttcccccttg ggcggcggac cggcatagtg gctggcgagt tcgcccagcg tgccgcggcg
     2941 gacctcctca tgcagcttgg tcaactcgcg cgccacggca gcatcgcgtg aacccaggat
     3001 ttcggtcatg tcggcgagcg attcccccag acgttgagcg gattcgtaaa agactagagt
     3061 cgcggaaatg gccttcagtc ctgcgatgcg gctacgccgt tccccggctt ttgaaggcag
     3121 gaacccggca aaaaagaacc gatcggtcgc tagtcccgca cagaccagac cggcaagcac
     3181 ggcggaggct cccggaatcg gaaaaaccgg caattcctcc tccaaagcct cgcggaccaa
     3241 tttatggccg ggatcggata tcagaggcgt gcccgcatcg ctgaccagcg ccagccgcgc
     3301 gccggtccgc agcttcgaca acaatttcgg acgcatcgaa gcggcattat gatcgttgta
     3361 cacaaagaga gggcggctga tgccataaag cgagagcagg cgggatgtga cgcgggtgtc
     3421 ttccgccaca atcgcatcgc aatgtttcag cacagtcagc gctctcagcg tgatatcggc
     3481 cgcgttgccg attggagttg ccgttatata gaggccagga gcaaggacgg gcgcgatgga
     3541 cgtttcccac gattccccgg tgcccgaggg atgggattta ccctttcctg tctcgcgctt
     3601 agggtcgcgg ttcgattctt cgggggcgcc tgcgtccgca tgggaatgag tcggtggcgc
     3661 cacgaaggga gcagccgggg atgaatgttt tcgcgtgttt ttccggtgac gtgccaaatc
     3721 cggccggtcc tctcaaggcc attactcggc tgcgcgaatc gcgcggcaaa gtgtgcgcga
     3781 ttgcatgcac ggttgcgata ggggcgctgc ttgccggttg ccagaccatg aagccggtgg
     3841 ccgcaccgcc gccgccgcct cccccccctc cgatgcgcat ggtgatggcc ccgccaccgc
     3901 ccccgccatc gcgtatggag caacccaatt tctaccgttt gcgcaattcg ccgtccggtg
     3961 tggtccccgc tcgcgtggca ctcttgcttc ccctttcagg ggggacggcc gaaacccgcg
     4021 cggtcgccga ggcgctggag aaagccgcgg aaatggcggt gttcgattcc aagaacaccg
     4081 acatcctgtt gatgccgcgc gacgatggcg ggaccccggc gaaagccgcc gcggcggcca
     4141 ccaaagccat caatgacggg gcggagatca tcattggccc gctgttcgcg gcattggtga
     4201 cggcggtgac ccccgttgcc cgcgccaacc acgttccggt gattgcgctt tcaagcgacc
     4261 gctcggtcgg cggcaatggg gtctatctct tgagcttcca gccggaaacc gaggtcaatc
     4321 gcatcatttc ctacgcggtc aggaccggcc acagccgctt tgccgcaatg atccccaaaa
     4381 acgcctatgg cgaaaaggtc gaagcggcct ttcgcgattc ggtgacgcgt gcaggcgcga
     4441 atgttgcgga cgtggaggct ttcgacgaaa gaccgcaggt ggttgggcct ccggccaagg
     4501 ctgccgcgca atcgggcgcc gacgcgatcc tgattgccga tgggggcgcc atgctccagg
     4561 ccatcggccc ggcgctcgca ctcaacggcg ctaccagccg caccatgcag ttcctgggca
     4621 cggggctgtg ggacgatgcg tccatcgcgc gcgaaccggt gcttgccaac ggcagattcg
     4681 ccgccccacc gccggaatcc ttccgcagtt tcgccgcgca ttaccgagaa atctatgagg
     4741 ccgcaccacc gcgcatcgcg accctgtctt atgatgcact gtcgctcgtc gcgttgttaa
     4801 cccggggccg gccctatcag cgcttcacgg atgcggccct gaccgacccg aatgggttct
     4861 ccggcgtgga cggcattttc cgcttccgcg ccgatggttc ggccgagcgt gggcttgcca
     4921 tcctccaggt gtcgcagggc ggtttttccg tgatcgaccc ggcgccaaaa tcctttccgc
     4981 agcctgggtt ttaggcgaac tggggttcga gagcctcggc caatttccca atgatgccgt
     5041 tcagcaccag gcgtcctctg ggagtcgcct tgaggcggcc ctcgtccagc gcgatgagtc
     5101 cagccccctg tagcgcctca agacgcactg gcgaaggctc gacattccag cgggcacggt
     5161 aggaggcgat gtccaatcct tcggtcaacc gaacagacat cagcaaatgc tcttgggccg
     5221 cttcgttatt gttcacatcc gtcatgacaa aactcgagcc ccggcgttcg acctcctcca
     5281 gccagcgttc cggcaggcgt tcggcaattg tcgccaaacg tcgttcggag agcgttagcc
     5341 ggccatgggc cccgggaccg gtgcccacat aatcgccgta acgccaatag gttaggttat
     5401 gccggctttc cgctcccctc tgcgcatgat tggagatttc ataggcagga agccccgctt
     5461 tatcgcaaat ctcctgggtc agctcataga gttccgcggc gcggtcctcg tccggaacaa
     5521 tcagtttgcc ctgccgcgcg agcgtggcat aggcggtacc ttgttcgatt gtcagttggt
     5581 aaagcgatag atgttcggtt ccgaaggaaa gtgcctcttt cagctccaac gcccatgccg
     5641 catgatcgtg ctcgggccgc gcatagatca gatcgaagct cacgcgcggg aagatcttca
     5701 aacctagccg gatcgcgcgc cgcgcttctt ccacgccatg cagccgcccg aggcccttga
     5761 gcgccgtctc atccagggcc tgaacaccga tggagacgcg attcacccca gcggcacgat
     5821 aatccgcaaa gcggctctgc tcgacgctgt tgggattggc ttcgagcgtg acctcggcgt
     5881 caggcgtcac ccgccagaac tgcgcgatcg cgtcgaggac cgcgcctacc gcgacaccgc
     5941 ccatcagcga gggtgtgccg cccccgaaga aaatacttgt cacttccgga cggttgctgc
     6001 cttgcaacgc tgccgctgcc cgaagttcgc tcgcgacggc acgcgcccag cgcatctcgt
     6061 cataacgctc gcggacatgg gagttgaagt cgcaataagg gcatttggcg gcacagaacg
     6121 gccagtgcac atagatgccg aaggcgttca ttgttccgta tccggagcaa ccgccccggc
     6181 gacgaatttc gcaaaggcgc gcgcacgatg cgacatcgcg tgctttgccg agggggccat
     6241 ctcgccgaaa gtgaggcggt gaccttccgg aatgaagatc ggatcgtagc cgaaaccctt
     6301 gtcgccgcgc ggcggaaaag caagggcgcc gaacacacgt ccttcgaaga tttgcgtcac
     6361 cgcgcccggt gccgcaagcg ccagcgcaca cacgaaatag gcgcgcgcat cgtgcgtatc
     6421 tttctcgcgc agctcggttt ccacgcgccg catggcgagg cggaaatcct tctgcggtcc
     6481 tgcccagcgc gcagaataga tgccaggtgc gccgtcgagt gccgccacgc agagacccga
     6541 atcgtcggag agcgccggca agccgcacgc acgcgtcgcc gcatcggctt tcaacgcggc
     6601 attttgcgca aaactcgttc cggtttcttc cggctcggga agaccaagct cagccgcgcc
     6661 caccgtctcg atgccgaacg gcgcgatcaa tgccctgatc tccttcactt tgcccggatt
     6721 gtgactcgca accacgagcc ggtccccgga cttcagccgg atcacagctt caaagcctgt
     6781 ttttgaagat ccatcaattc cgcgatgcct ttgcgggcga gccccaggag ctggccgaac
     6841 tcggcctcgc tgaagggcac gccttcggcg gtgccctgga tttccacgat cccgcctgca
     6901 ccggtcagaa caaaattggc atcggttccg gctgtggaat cctcggcgta atcgaggtcg
     6961 agcaccgggg tgttttcata gatgccgcag gacaccgccg cgacatgatc cttgatcgca
     7021 tggcgcgcga tcagcttggt tcccgccata aagctgatgc actggtagag cgcaacaaat
     7081 ccacctgtga tggaagcggt gcgcgtaccg ccatcggcct ggatgacatc gcagtcgatg
     7141 atgatttgcc gctcgcccaa aagctcgagc gcggtgaccg ctcgcaagga tcgtccgatc
     7201 aggcgctgga tctcctgtgt gcggccggtc tgtttgccgg tcgccgcttc gcgccgggtc
     7261 cgctcggagg tggcgcgcgg cagcattcca tattcggcgg tcacccagcc gcggcccgaa
     7321 ccgcgcagaa agggaggcgc ctgattctcc agactggcgg tgcacagcac gtgggtatgc
     7381 ccgaatttga ccagacatga gccttcggcg tggcggagca caccgggttc caggttcacc
     7441 tcgcgcatct cgtcgttgcg gcgggcggag gggcggaggg aggggcccat ggaagctctc
     7501 ttgaagttgg gggtcgccac atttacctag cggccatgac ctgtcagtgc aaatgcgcgg
     7561 ccgaaaccgc gaaaacaggt tagaacggtc aagaatttag cagtttcaac acgatggaag
     7621 ccaggacacc caaggccatg gccggcgatt ttgtcgcaag cgaacttggc agccgcccgc
     7681 gcgaggtctt tcgccatctg gtggaggcct atctgtcttc cggcgagccg gtcggctcgc
     7741 gcacgctgtc ccagcgcctg ccgctgacgc tctcgcccgc gtccatccgc aacgtcatgg
     7801 ccgatctgga aggcatgggc ctgctctatg caccgcacac gagcgcgggc cgggttccaa
     7861 cggagaaggg tttgcgcctg ttcgtcgacg gcatgctgca gattggcgat cttacttcgg
     7921 cggagcggtc ctctatcgag gaccgtatca aagcctcggg gcggccgttc gaggatgtgc
     7981 tgacccaggc agcgagtctc ctgtccggcc tctcgcgctg cgccggcttg ctggttgcgg
     8041 ccaagcagga gaccgcgctc aagcatatgg aatttgtcgc gattggcccc ggcaaggcgc
     8101 tggcgatcct gattggcgca gacggcagcg tggagaaccg ggtaatcgat acgccgttgg
     8161 gcctcttgcc ctcggccttg accgaggccg ggaactatct ctcaagcaag ctccagggcc
     8221 gtacgctgga atcggccaag gccgagattc tcaaggagat cgaggaggaa cgtacggaac
     8281 tcgatgtgct caccgcgcgc atcgtggcgg aaggactcgc gaccctttcc gcgcgcgctc
     8341 cccgcgaggg tgccgatgcc gcgggcgacc ggatcctgat cgtccgggga gcctcgaacc
     8401 tgctggacga catcgaagcc cagggcgatc tggcgcgcgt ccgcaccttg tttgacgata
     8461 tcgagcgcaa gaacgacctg atcgggctcc tggaactcgc ccgtcaaggc gaaggcgtcc
     8521 acattttcat cggctcggaa aataaactgt tttccttgtc gggctcttcg atcgtcgctg
     8581 ccccctatgc cgattccaaa ggcaaggtgg tgggagtcat tggggtcatc ggcccaaccc
     8641 gtttgaatta tgctcgcatc attcccatgg tggattatac agcgcgcgta gtcggccgcc
     8701 ttctggcata gacgataagc gggccgacgc gttaaggagg atcatgtcag cggaaaacga
     8761 caatcagccc ttgccggagg acatcggtca ggagaccctg tccgagcagg aggcggttca
     8821 cggcgtcatc aacgcgctgc gcgtcgaagc cgacgcgcag aaagaccgcg cgcttcgggc
     8881 cctggccgag gtggagaatg tccgcaagcg tctcgaacgc gagcgcgacg aggcgcgcat
     8941 ctatgccgtc acgcgtttcg cccgcgatct cttgacggtc ggtgacaatc tctcccgtgc
     9001 gctggccgcc gttcccgccg atgtgcgcgc ccaagccggt gattcgctga cggcggtgct
     9061 ggatggcgtc gaagccaccg aacgcgagct gaaagccacg cttacgcgcc atggcgtgaa
     9121 acccatcgag gccgaaggcg tgcggttcga ccctcatctg catcaggcaa tcgccgaggt
     9181 gccggcagaa ggagccgagc ccggtaccgt catgcacgtg gtgcaaccgg gttacacgat
     9241 tgcggaacgc ttgctccgtc cggccatggt gacggttgcc aaaggtgcgc ctcggcccaa
     9301 tggcggcaac ggcgccggca gccagatcga ctccaagatt tgatctttcg tgctagtttg
     9361 gcgggcgaaa tccgagggcc atccatgaat cccattatcc gcttggcgcg ccccggctat
     9421 atcaccggca gcgcccaggg cgcgtttatg gttgccgtgc tggaagccgt gctcggggcc
     9481 tcgttgcaat atttcaaggt ccggcgcgcc gggcgtgtca tgcgccgcgc atgagcagga
     9541 ttcgtattgc cgttgccggg gcctcgggcc gcatggggcg gacgctcatt cgcggcctcg
     9601 ctgaaaatcc cgacatgaca ttcgccttcg cgctggaacg gccaggtcat gcgtcgctcg
     9661 gcacggatgc cggcgtgctc gccgggttgc cacccaatgg catcgccgtt accggcgaca
     9721 gtctcgcggc actggccgcc tgcgatgcgg tgctcgattt caccgcgcct tcgtcaagcg
     9781 tcgccttgtc cgaactggcc gcgcaagcgc gcatcgtgca cgtcatcggg accacgggtt
     9841 tttccgacgg cgaattgggg cgtctcaagg ccgccgcgcg tcatgcggtc atcgttaaat
     9901 cgggcaatat gagcctcggg gttaatctcc ttgcggcgct ggtgcgcgaa gcagcccggg
     9961 cgcttcccgg ctacgacgtc gaaattctcg agatgcatca tcgcatgaag gtggatgcgc
    10021 cctcaggcac ggcgctgctt ctgggcgaag ctgccgcgca aggccgggac atcgcgctga
    10081 aagatcgtgc ggtgcgagtc cgcgacggcc agaccgggat ccgcccggaa ggcgccattg
    10141 gatttgcgtc cctgcgcggt ggcacgattg tcggcgacca catggtcgtg ctggcggggc
    10201 catcggagcg catcgagctg acccatcgcg ccgaagatcg catgatcttc gccaagggcg
    10261 cgctggccgc tgcgcgatgg ggacagggca agaaacccgg actttatgcc atgagcgacg
    10321 ttctgggtct ttcgcgctaa aatagcctgg tcgcgcagag gagttcgttc gtttgcggct
    10381 cacgattttc atcctcgcgg ccctaaccgt cctcgctggc ggggccgcgt ctgccgcaac
    10441 accggaggag acttccgcgg cgtgcgcggc cgacgatccc gacgtcgcca tccttgcctg
    10501 cagcgccttg atcgaatcag gacgtgaatc gccggccgat ctgtcgcgcg attacaacaa
    10561 tcgtggtggc gccttctcgg ccaagggcga ttatgaccac gcgatcctgg atctcgacga
    10621 agcggtgaag ctcgacgcgc agaatatcgg cgccttgatc aatcgcggca attcctatgg
    10681 cggcattcgc caatatgacc gcgcgctcga ggattacagc gcggcgctga agctcgatgc
    10741 caatgattcc cgcgcctatg aggcgcgcgg cggcgtgtat gaaaaactgg atcggccggg
    10801 agacgccttg gcggattacg ataccgccgt tctattggat gcccataata tcgacgcgct
    10861 gatcagccgt ggcgttcttt acaggagcca gggagaagtc gaagcgggga tcgccgattt
    10921 taccgccgcg attgctttac acccggattt cgcgacggcg ttcgacaatc gcggttttgc
    10981 ctatgcgatc cagcaacgct acgaacacgc gctggccgat tacgacgagg ccgtcagatt
    11041 gaatcctcat gcgatggtta cgctttatca tcgcggagtt
//
LOCUS       DASYEG010000282         3679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4313458, whole genome shotgun sequence.
ACCESSION   DASYEG010000282 DASYEG010000000
VERSION     DASYEG010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4313458"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /gene="hemC"
                     /locus_tag="VGM72_11930"
     CDS             <1..791
                     /gene="hemC"
                     /locus_tag="VGM72_11930"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY4266020.1"
                     /translation="LVEAGGKGLFVKEIEEALINGDADIAVHSMKDMPALQPPGLFVV
                     AVLPREDPRDLFIARDGAPFDKLAEGARLGTSSVRRQAQALRLRPDLKIVPLRGNVET
                     RLLKLARGEADAIMLARAGMSRLKLALPDSEILESDHWLPALCQGAIGIECRNDDSTT
                     GQLLAAIDDAPSHLAIACERGFLAGLDGSCRTPIAGLAEIEDGRLAFRGEVLSMDGRN
                     SWSVSRDIGFDSGMGEYARTQAYAAGQDAAREIRERAGTDLPRF"
     gene            833..1537
                     /locus_tag="VGM72_11935"
     CDS             833..1537
                     /locus_tag="VGM72_11935"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY4266021.1"
                     /translation="MTRPRDDAQSFAEFLRARGHEAVIAPIMEVQFPPGPPLALDGVQ
                     GVLATSANGVRALSFRTARRDLTIYAVGPQTAEAARQSGFTVVISADGNAAALVETVA
                     READPAKGPLLHAAGAETAGRLRQALQARGFRVESAVLYEALPVTRLPAEAQDALRNN
                     ILDGVLIFSPRSAKTFATLTGAAGLSAQCAALIAFCISAATAEALAPLTFARVVIAGT
                     PNQDAILDLIPAEPAR"
     gene            1596..2570
                     /locus_tag="VGM72_11940"
     CDS             1596..2570
                     /locus_tag="VGM72_11940"
                     /inference="COORDINATES: protein motif:HMM:NF021264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitofilin family membrane protein"
                     /protein_id="HEY4266022.1"
                     /translation="MSETQSASTPRPEREGPSSAPSPAPQPGAAAKSGRGRRFSGFLA
                     IAGLMLLAGAATLTVERLRSDHVLATNLAEISRDLAALKSTVEALNARTGVLEHSASE
                     VQAADTQLAGLAMRLSVLEADLGSGADRDTLAQLQDRIARLESRSPDEMLKTAATALA
                     RANLARAAEGDAPLNAELEALRTVDPDDPALGPLQVAADNGVATRATLAAAFPEAARA
                     ALDAAYRGEETGNFVRRLWASMRRLISVRRVGDTAGLTTEDRLARAQADLDRGGLVGA
                     VTEVRGVGGAAAGALAPWLKTAEARITQDRAVAEMNARIVETLAAPRP"
     gene            2567..2878
                     /locus_tag="VGM72_11945"
     CDS             2567..2878
                     /locus_tag="VGM72_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266023.1"
                     /translation="MMRLLFILIIAGVAALAAAWVADHDAVLTLTVADYEIRTTAAVA
                     VVILLVCFFVLRVALRILFAVLKGLAMAGGQLSRNHRNATGVSRASTDAKIPLPREQP
                     S"
     gene            2963..>3679
                     /locus_tag="VGM72_11950"
     CDS             2963..>3679
                     /locus_tag="VGM72_11950"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY4266024.1"
                     /translation="MNIHEYQAKEVLRGFGAPTPKGRAAFSVEEAVAAAQDLGGPVWV
                     VKAQIHAGGRGKGGGVKVVRSVEDVKREAARMLGMTLVTHQTGPQGRVVRRLYIEDGS
                     DIARELYLSALVDREASRVAFIASTEGGMDIEEVAKHAPEKIQTFHVDPAAGLSAHTG
                     RSIAAALKLEADQAKQAGAVIESLYKAFIAKDMSLLEINPLVVTKSGQVICLDAKVNF
                     DDNALYRHKDVQAMRDPDEED"
BASE COUNT          615 a         1192 c         1242 g          630 t
ORIGIN      
        1 cgctggtgga ggcaggcggc aaggggcttt tcgtgaagga gatcgaggag gccctgatca
       61 atggggacgc cgacatcgcc gttcattcga tgaaggatat gcccgcgctg cagcctcccg
      121 gacttttcgt cgtagcggtg ctgccgcgcg aagacccgcg cgatctcttc atcgcgcgtg
      181 acggggcacc gttcgacaag ctggccgaag gcgcacggct gggcaccagc tcggtccgcc
      241 gccaggcgca ggccttgaga ttgcgtcccg acctgaagat cgtacccttg cgcggcaatg
      301 tcgaaacccg gcttttgaag ctcgcgcgcg gcgaggccga tgcgatcatg ctggcgcgcg
      361 ccggcatgtc acggctgaag ctggcgctcc ccgacagcga aatcctggaa tcggaccatt
      421 ggctgcccgc gctttgccag ggcgccatcg gaatcgagtg ccgcaacgac gattccacga
      481 ccggtcagct ccttgccgcc atcgacgatg cgccgtctca tctcgcgatc gcctgcgagc
      541 gcggctttct cgcgggcctc gatgggtcct gccgcacacc tatcgctggt cttgccgaga
      601 ttgaagacgg acggctcgcg tttcgcggtg aggtcctgag catggacggc cggaactcgt
      661 ggtcggtatc gcgcgatatt ggtttcgaca gcggcatggg agaatatgcg cgcacgcaag
      721 cctacgccgc cggccaggat gcagcccggg aaatccggga gcgcgcaggc acggatcttc
      781 cgcgtttcta gcgtgacgat ggtttggatg tgacgacgat atgcgccttc tggtgacccg
      841 gcctcgcgac gatgcgcaaa gtttcgcaga attcttgcgc gcgcgcggcc atgaggcggt
      901 catagcacct atcatggagg ttcaatttcc accgggtccg ccgcttgcgc tcgacggcgt
      961 gcagggcgtg cttgcgacga gcgctaatgg cgtgcgcgcc ctgtcgtttc gcaccgcccg
     1021 gcgcgatctg acgatctacg cggtgggccc gcaaaccgcg gaagccgccc ggcagtcggg
     1081 cttcaccgtg gtcatcagcg ccgatggcaa tgccgcggct ctggtcgaaa cggtcgcgcg
     1141 cgaggccgat ccggcaaagg gtccattgct tcacgcggcg ggcgcggaga cggcaggccg
     1201 tctgcgccag gcgctgcaag cgcgcggctt tcgagtcgaa tcggccgtgc tgtacgaggc
     1261 gctgccggtg accaggcttc ccgccgaagc ccaagacgcg ttgcgcaaca atatactgga
     1321 cggtgtcctg atattctcgc cacgcagcgc gaagaccttc gccacgttga ccggcgccgc
     1381 aggtttgtcg gcgcaatgtg cggcgctgat cgccttctgc atcagcgctg ctaccgccga
     1441 ggcgctcgcg ccgctgacct tcgcgcgtgt cgtcattgcc ggcacgccca atcaggacgc
     1501 catcctcgac ctcatcccgg ccgaaccggc gcgttaatct tgcgccaggg cgggtgtcgc
     1561 acctatagcg taggagccct tgcgggaaga tggccatgag cgagacgcaa agcgcttcca
     1621 ctccgcgccc cgagagggaa ggcccatcgt cggctccttc acctgcgccg cagccgggcg
     1681 ccgcggcgaa atcagggcgg gggcggcgat tttcgggatt tcttgcgatc gccgggctca
     1741 tgctgctggc gggggcggcg accctcacgg tcgagcgtct tcgctcggat catgtcctgg
     1801 ctacgaatct cgcggaaatt tcgcgcgacc tcgcggcgct gaaaagcact gtcgaggctc
     1861 tgaacgcgcg caccggcgtg ctcgaacact ccgcgtccga agtccaggcg gccgataccc
     1921 agcttgccgg cctggccatg cggctcagtg tgttggaagc ggatctcgga agcggcgcgg
     1981 accgcgatac gctcgcccaa ttgcaggacc gcatcgcgcg gctggaaagc cgcagcccgg
     2041 acgagatgct gaaaactgcg gccacagcct tggcgcgcgc caatctcgcg cgcgccgcgg
     2101 aaggagacgc gccgctcaat gcggaactcg aggcactgcg aaccgtcgat cccgacgatc
     2161 ccgctcttgg tcctctgcag gtggccgcgg ataatggcgt ggccacgcgc gccacgctgg
     2221 ctgccgcgtt tcccgaagca gcccgcgcgg cgctcgatgc cgcgtaccgt ggcgaggaaa
     2281 ccggcaattt tgtccgccgg ctgtgggcga gcatgcggcg gctcatcagc gtgcggcggg
     2341 tgggcgatac cgcagggctc accaccgagg atcggctggc ccgcgcgcaa gcggatttgg
     2401 accgtggcgg cctagtgggg gccgtcacgg aagtgcgcgg cgtcggcggc gctgcggccg
     2461 gggcgctcgc tccctggctc aagaccgccg aggcgcggat cacgcaggat cgcgccgtgg
     2521 ccgagatgaa tgcgcgcatt gttgaaacct tggcggcgcc gcgcccatga tgcgccttct
     2581 tttcatcctg atcatagcgg gcgtggcggc gctggccgct gcctgggtcg cggaccatga
     2641 tgcggttctg accctgacgg tcgcggatta cgaaattcgc accaccgccg ctgtggccgt
     2701 cgtgatcctg ctggtctgtt ttttcgtgct ccgggttgcg ttgcgcattc tgttcgcagt
     2761 cctcaagggc ctggcgatgg caggcggaca actttcccgg aaccacagga acgcaaccgg
     2821 ggtgtcgcgt gcgagcaccg atgccaagat cccgctgcca cgggagcagc cctcttagag
     2881 cggaactgtt cttgccgcac cggcgcggct gctataactc gtttccgtct cccgtggcct
     2941 cagattcggt gtccgcagcg ctatgaacat ccatgaatat caagcgaagg aagtcctgcg
     3001 cggttttggg gcgccgaccc caaagggacg cgccgccttt agcgtcgagg aagccgtagc
     3061 cgcggcccag gatctgggcg gcccggtctg ggttgtcaaa gcgcaaatcc atgccggcgg
     3121 acgcggcaag gggggcggcg tcaaggtcgt gcgctcggtc gaggacgtga agcgcgaagc
     3181 ggcgcggatg ctgggcatga cactcgtcac ccatcagacc ggaccgcagg ggcgcgtcgt
     3241 gcgccgtctc tacatcgaag acggctccga catcgcccgc gagctttatc tgtcggcgct
     3301 ggtggatcgc gaagcctcgc gcgtcgcctt catcgcctcg accgaaggcg gcatggacat
     3361 cgaagaggtg gccaagcacg cgccggaaaa aatccagacc ttccatgtcg atcccgccgc
     3421 agggctcagc gcgcacaccg ggcggtccat tgccgcggcg ctgaagcttg aagccgatca
     3481 agccaagcag gcgggagccg tgatcgaaag cctctacaaa gccttcatcg cgaaggacat
     3541 gagcctgctc gaaatcaatc cgctggtggt gacgaaatcc gggcaggtga tctgcctcga
     3601 tgccaaggtc aatttcgacg acaacgcgct ctatcgccac aaggacgtgc aggcgatgcg
     3661 cgatcctgac gaggaagat
//
LOCUS       DASYEG010000283        23831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1266556, whole genome shotgun sequence.
ACCESSION   DASYEG010000283 DASYEG010000000
VERSION     DASYEG010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 23831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23831
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266556"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..243
                     /locus_tag="VGM72_11955"
                     /pseudo
     CDS             22..243
                     /locus_tag="VGM72_11955"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
     gene            352..555
                     /locus_tag="VGM72_11960"
     CDS             352..555
                     /locus_tag="VGM72_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEY4266025.1"
                     /translation="MGDGHVKFFNEQKGFGFVVPESGGTDVYVHASALRRSGVPTLMP
                     EQRVRFSTRQGMKGVEVDRIELA"
     gene            complement(581..1846)
                     /locus_tag="VGM72_11965"
     CDS             complement(581..1846)
                     /locus_tag="VGM72_11965"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4266026.1"
                     /translation="MRLKFRLGTMVLALLLCASLDAQAATKWVRFGKLWDGHRALANA
                     VVAVQDGKIQSVTANGTIPSGAETVDLSRYTGLPGLIDSHTHMTFYWDRSTGATPLHQ
                     PPRHVAVTVFLAQENARKTLEAGVTTVRDLNGLDGADLALRDLINRGAVIGPRMFVSG
                     AGIRYMTYRHPGVTDPVAEAVKLTKSLLASGVDWIKIYASTGIADDLTGEQTVSYDEM
                     KAIIDTAHAAGRKVAVHSYGPAAARDAIRAGADTLEHATDMDDATIAALVRNKMWYVP
                     TINHNQHYLDRADDVYKFTPETKENLRNFIQRNFVTAQKAYRAGARILVGSDAVYTAF
                     GTNMQELSWFVKLGMSNEQALQSATILPAEMLGMEKSLGAVAPGYLADMVAVEGDPLA
                     DISVVPNNVRWVMKAGDVVVDKTGTVRER"
     gene            complement(1857..2129)
                     /locus_tag="VGM72_11970"
     CDS             complement(1857..2129)
                     /locus_tag="VGM72_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266027.1"
                     /translation="MRKPWFFALVLAGCANDGPGDPNYDIAKAMETITQNAMIGYHDR
                     CLSFGAEIDTPLYAQCVSTLAEADKQAEELREGQEGSAGERRSDQR"
     gene            complement(2201..4033)
                     /locus_tag="VGM72_11975"
     CDS             complement(2201..4033)
                     /locus_tag="VGM72_11975"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEY4266028.1"
                     /translation="MPYLWPPGRPDLKWRVAVSLIFLVLTPIVTVATPIFLGRVVDGL
                     QGRPEDIALFVPIAFIVSYGVAWIMSQAVAQIRDGIFARPCYHALRVMAVETFHHLHA
                     LSLRFHLQRHTGGLSRIIDRGTKAVDRLLTLAIFNIFPTGLQLILICLYLWAQFNAWF
                     TTATVAMILIYAYFTFAVTRRRIQTRRVMNDSDNEANSKAVDSLLNYETVKYFGAELH
                     EEKRFDAAMTKYSDASIVTQISLSMLNTGQRVIAASGVVALMILAAIGVKNGSMKVGD
                     LVMVNSLLIQLFIPLNLLGTVYRDISQGLIDMETMFNLLRRPQEIQDREGAQPLAIAH
                     GDIAFDNVVFAYEPDRIILKGVSFRVPAGKTVAVVGPTGAGKSTISRILYRFYDIQSG
                     SVTIDGQDIRDVTQASLRAAIGIVPQDTVLFNDTIRYNIAYGRIGANEPDILEAARLA
                     QIDPIIRELPTGYDSMVGERGLKLSGGEKQRVAIARTLLKNPPILLLDEATSALDTHT
                     EREIQSSLKLVSKNRTTLVIAHRLSTIIDADEILVLDRGQIVERGRHGELIARGGAYA
                     AMWNRQKEAAEVRERLQAVEGDPEDLRGAVTDPKRRVVAGGAAE"
     gene            4269..4871
                     /locus_tag="VGM72_11980"
     CDS             4269..4871
                     /locus_tag="VGM72_11980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY4266029.1"
                     /translation="MNRMPSSLTPARPTICVFCGSSHGANPVYTEAARRFGTLMVEEG
                     FDLVFGGGGVGLMGETALAVSKAGGKILGIIPSFLRHVEPPLRVASEIVVTESMNERK
                     ARMFEAADGFAVLPGGLGTLDEFAEAFTGAQLRLHAKPIVLVNIQNYFAPLVRLIDHF
                     VTEGFAGPGVKDLFQLAPTVEDAIAIFAAHEAKTIKQPAE"
     gene            complement(4872..6470)
                     /gene="cimA"
                     /locus_tag="VGM72_11985"
     CDS             complement(4872..6470)
                     /gene="cimA"
                     /locus_tag="VGM72_11985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEY4266030.1"
                     /translation="MSRERVYLFDTTLRDGAQTQGVDFSVEDKRTIALALDSLGIDYV
                     EGGWPGANPTDTAFFNDPPALAQARFTAFGMTKRSGRSADNDPGLAALLSTKAQAICI
                     VGKSWDFQVDVALEIPRQENLDNIAQSIAALVAKKREALFDAEHFFDGYKANPDYAFA
                     ALRAAFDAGARWLVLCDTNGGTLPEDAYAIVSEVKSRLPGANFGVHAHNDTEQAVAVS
                     LAALRGGARQIQGTLNGLGERCGNANLCSILANLMLKEPYASQFQTNVTAEALTRLTP
                     TSRLLDEILNRAPNRHAAYVGPSAFAHKGGLHVSAVQKNPKTYEHVTPESVGNQRHIL
                     VSDQAGRSNIMARLVLAGIEVSPDDKRIGRLLEDVKAREFLGYAYDGAEASFELLARR
                     ALGQVPNYFQVDSFRVIVERRHNALGELVTMSEAVVRLNVRGESLHNVGTGNGPVNAL
                     DSALRKDLGHYSSYLQDLRLVDYKVRILTGGTEAVTRVMIESADGHGHRWSTVGVSEN
                     IVDASFEALSDSICYKLYRTGAPA"
     gene            complement(6467..8077)
                     /gene="cysS"
                     /locus_tag="VGM72_11990"
     CDS             complement(6467..8077)
                     /gene="cysS"
                     /locus_tag="VGM72_11990"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY4266031.1"
                     /translation="MNLRLYNTLTRTKEDFVPLDAKNVRMYVCGPTVYDFAHIGNARP
                     VIVFDVLFRLLRHLYGAEHVTYVRNITDVDDKINARASEIAGERGLSVEAARSELTAR
                     TVQQFQDDVAALGCLPPTHQPRATEHIAAMIAMNERLIAAGHAYAAEGHVLYDVSSKP
                     DYGKLARRSLDEMMAGARVEVAPYKKSPMDFVLWKPSTPEQPGWDSPWGRGRPGWHIE
                     CSAMSESLLGETFDIHGGGIDLVFPHHENEIAQSEGAHHGHALAKVWMHNGFLQVEGE
                     KMSKSLGNFVTIRELMRGWASTAWSGSVIRLAMLRSHYRQPINWTSESLAEAKRELAT
                     WVGVIRGFISRAQIKEREVGNVPTPDFVSALLDDLNLPEALTALRTQHSAAARGDLES
                     ATSLYKNLRFLGLIDMQKAAAYIQGVSSAGPLTHEKLFENYDLGDSLKTAYLNNDTEF
                     QNQIGVELQKRNIKAFIDRLGVTLQLIDETVKVDPKPDYIQELIAARNEARKAKNFKE
                     SDRIRDELLAKGIVLKDGPTGTTWEVKR"
     gene            complement(8270..9943)
                     /locus_tag="VGM72_11995"
     CDS             complement(8270..9943)
                     /locus_tag="VGM72_11995"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEY4266032.1"
                     /translation="MNAKAPNAFAIALAQLNPVMGDIPGNLARARAAREKARAAGADL
                     ILFSELFITGYPPEDLVLKPAFQTDAMEAVENLAKDTADSGPAVLIGTPWVKDGALYN
                     AVAYLDAGKVAGTSFKVDLPNYGVFDEKRVFAPGPMPGPFNIRGVRIGVPICEDIWGG
                     DPVECLCETGTEILLVPNGSPFDALKRDHRLNVTVAHVVECNLPLAYLNQVGGQDELV
                     FDGGSFVLNADRSLAVQLPSWEEALSITHWRREGEGWVCEQGPLAEMQDQNMALYSAM
                     VLGLKDYVNKNRFPGVVLGLSGGIDSAISAAVAVDALGASRVRAVMMPSRFTSKDSLE
                     DAAAVAKLLGIAYDIVPIEEPMRAFGEALAPIFAGRVADATEENIQARIRGVILMAIS
                     NKFGPMVLTTGNKSEMSVGYATLYGDMAGGYNVLKDLYKTQVFALSRWRNQTWPNGAL
                     GPQGRMMPERVIDKPPTAELRADQKDEDSLPPYDVLDSILELLVEGEKSFEEVVAKGY
                     DPAIVRRVEQLLYVAEYKRRQAAPGVKLTTRNFGRDRRYPITNAFRDAR"
     gene            complement(9991..10926)
                     /locus_tag="VGM72_12000"
     CDS             complement(9991..10926)
                     /locus_tag="VGM72_12000"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4266033.1"
                     /translation="MRAAIVGAGVFGRHHAAKYLRMQGVKLDAMADPSAEARHFVETQ
                     LEVPVVADWRELLGKVDIVSVCSPASTHAEIVRAFLNAGAHVMVEKPIATDVEEADAL
                     IELAAARGLVLTVGHQERFVFAKSGLLDYEDAPLSIECVREGTWTGRGTDVSVVLDLM
                     IHDLDLIHRLVPGGIAGVRAEGRVLCGTLTDQVTANLVFENGSEVELVANRAADARRR
                     FMRMVYPDGEIEIDFLTREVTNTTSRKLQNMEVRDPLWESVAAFATAARAGASTLVRP
                     EEACRALETALLIEEALVPSGEILKPKSSPLRRTA"
     gene            complement(11098..12486)
                     /locus_tag="VGM72_12005"
     CDS             complement(11098..12486)
                     /locus_tag="VGM72_12005"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:TIGR01358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HEY4266034.1"
                     /translation="MAKPWTPSSWRGMPIKQVPQYPDAAALAAVEARLKSYPPLVFAG
                     EARNLRAALGQVADGKAFLLQGGDCAESFAEFHPDNIRDTFRVILQMAAVLTFAGAMP
                     VVKVGRIAGQFAKPRSEDTETQKGVTLPSYRGDNINSIEFDAKGRVPDPDRLLQAYGQ
                     SAATQNLLRAFATGGYADLHNVHRWTLDFIAGSPASERYRALSARIGEALGFMEACGV
                     TPELVPQLRTTDFYTSHEALFLGYEQALTRIDSTSGDWYCTSAHLLWIGDRTRQHDGA
                     HLEFCRGIKNPIGLKCGPSLGEDDLLRLIDILNPENIPGRLTLICRFGADQAGEKFPK
                     LLRSVTREGKKIVWSCDPMHGNTIKASTGYKTRPFDRVMKETRTFFEVHRAEGTHAGG
                     IHLELTGKDVTECLGGPQEISEDDLARQYDTHCDPRLNASQALDLAFLVAENLREERK
                     SQFSPVKTAVIS"
     gene            complement(12666..13349)
                     /locus_tag="VGM72_12010"
     CDS             complement(12666..13349)
                     /locus_tag="VGM72_12010"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEY4266035.1"
                     /translation="MNEAQAKRVTFAVEGGRVSGLLQIPKAARACYVLAHGAGAGMEH
                     PFLAAMAEGLDQRDIASLRYQFPYMEAGGKRPDAPKLAQATACAAVAEAGRLLPGVPL
                     LAGGKSFGGRMTSQAQAAAPLSGVRGLAFLGFPLHPVGKPSRDRGNHLFDVHIPMLFL
                     QGTRDALAELAELEPLCASLGRLANLKLFKDADHSFHVRAKSGRNDAQVRNEMLDAFA
                     AWTDGVLAA"
     gene            13434..13700
                     /locus_tag="VGM72_12015"
     CDS             13434..13700
                     /locus_tag="VGM72_12015"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEY4266036.1"
                     /translation="MCRNIRTLFNFAPPASEGEIRASALQFVRKLSGFAAPSKSNDAA
                     FQRAVDEISEVVHRLFESMETNSPPKNREIEAEKARERAKLRFA"
     gene            complement(13811..15196)
                     /gene="gor"
                     /locus_tag="VGM72_12020"
     CDS             complement(13811..15196)
                     /gene="gor"
                     /locus_tag="VGM72_12020"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEY4266037.1"
                     /translation="MASDTYDFDLFVIGAGSGGVRAGRLAAQRGARVAVAEEDSFGGT
                     CVVRGCVPKKLFVYASSFAEEFEDARGFGWTMDGARFDWPALIANKDKDIARISGVYR
                     SVLENAGAAIFEDRAIFKDTHTIHLKKAARDVTAQTILIATGGYPSRQTEMVGAELCI
                     TSNEAFHLEKFPGSIVIVGSGYIAMEFAHIFHGLGAEVTLVCRGPKVLRGFDEDLRDS
                     LQASMRNKGIHLLLDTKLAACERVGKTLRATTTAGEIIDADQVMLAVGRRPNVMGLGL
                     EAAGVTTIASGHIDVDRYSRTAVPNIYAIGDVTGRLELTPVALHEAMCFVETVFGEKP
                     SAPDHELVATAVFTRPEIGTVGLSEERALKRGHTIDIYKTNFRPLKNTLSGRHERSMM
                     KLVVDAKTDKVLGCHVFSPDAGEMAQLVAIALKMGATKAQFDATVAVHPTMAEELVTM
                     RTKTATRSPSE"
     gene            complement(15233..15958)
                     /gene="rpiA"
                     /locus_tag="VGM72_12025"
     CDS             complement(15233..15958)
                     /gene="rpiA"
                     /locus_tag="VGM72_12025"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEY4266038.1"
                     /translation="MNDGEQQRLKREAAARALAFVEDGMKLGLGTGTTAELFLELLAE
                     RMRAGLKITGTPTSERTAALGRKLGIAMAELDALGTLDLVIDGADEADGNLDLIKGGG
                     GALLREKIVAASAKRMVVIADDSKLVDRLGAFALPVEVVAFGHGSTCARISAAAAKLG
                     YANLSPALRKKDGQAFHTDSGNVIYDCPFGEIADAKALAASLSAIVGVVEHGLFVNMA
                     SALVIAGAGGVRVIERRQRVSRR"
     gene            complement(16023..17855)
                     /gene="glmS"
                     /locus_tag="VGM72_12030"
     CDS             complement(16023..17855)
                     /gene="glmS"
                     /locus_tag="VGM72_12030"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEY4266039.1"
                     /translation="MCGIIGIAGTRDVAPLIVDALKRLEYRGYDSAGVATLNQGRLDR
                     RRSPGKLNQLVGLLAENPLPGFTGIGHTRWATHGAATELNAHPHASSRVALVHNGIIE
                     NFRELREELRAAGHRFESETDTEVAVHLITQYLDEGLSPEAAAKTAVKRLEGAFAMVM
                     IFKGEERLLIAARRGSPLAVGYGQTEAYLGSDAFSLAPFTNRVAYLEDGDVAVVRGAD
                     VMIYDSAGKWVARPIHIIPPSGAMVDKGNRRHFMAKEIHEQPEVVGHTIARYVDASGP
                     KVDLAPEAAALFRNAPRLTISACGTAFYAAMVGKYWFEKLARMPVDVDVASELRYRNP
                     VYSEGGLCLYVSQSGETADTLAAMRDAKAAGQTVAAIVNVPESSIARDADMVWPTYAG
                     PEIGVASTKAYTCQLAVLVALAIAAGRARGHIDEAEEKRLCGLLLEVPRHMVSLLVQA
                     QEAKIEALGLEIAKAQDVLYLGRGVHFPTALEGALKLKEISYIHAEGYASGEMKHGPI
                     ALIDGNVPVIVIAPHDELFEKSISNMQEVMARGGKVVLISDRKGLDQGADRTWATIEV
                     PETDALISPLLYALPIQLLAYHTAVAKGTDVDQPRNLAKSVTVE"
     gene            complement(17856..19235)
                     /gene="glmU"
                     /locus_tag="VGM72_12035"
     CDS             complement(17856..19235)
                     /gene="glmU"
                     /locus_tag="VGM72_12035"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:NF010933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEY4266040.1"
                     /translation="MNHPALAVIVLAAGQGTRMKSALPKVLHPIGGRPMLGHVLAVAQ
                     AMGAAQIVAVTAPGAEAVVALAGQWGGTSVVQERQLGTGHAVLAAENALAGFDGNLLV
                     LFGDAPLLTAATLSRLIAKLGAGGDLSALGFRAADPAGYGRMVTDGDSLIRIVEQKDA
                     SEDERRIDLCFAGMLAGRARLIFELLHRVGNRNAQSEFYLTDVIGIAQARGLRCAVVE
                     GPEGEMLGVNSRAQLAQGEAAFQARARAQLMDAGVGMTAPETVFLSADTIIEPDVVIG
                     QYVVFGPGVTIKRGADIRAFCHIENSAIGERAIVGPFARLRGGAKLDADVDIGNFVEL
                     KNAHLEKGVKAHHLTYLGDSHVGEKANIGAGTITCNYDGFSKSRTEIGAGAFIGSNAA
                     LVAPVEIGEGAIVAAGSVITENVAPNALAVARGRQETKPGRAVQIRARNKAKRDMAKK
                     DMAKKDRNV"
     gene            19401..20075
                     /gene="gph"
                     /locus_tag="VGM72_12040"
     CDS             19401..20075
                     /gene="gph"
                     /locus_tag="VGM72_12040"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01449.2"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEY4266041.1"
                     /translation="MPDTPLAIVFDLDGTLVDTAPDLLSALNAVLTAENHEPVVPSDL
                     RHIVGHGARAMFEHALLRTGTPVSAARLNTLTEKFLAYYRANIACGSRLFPGVPETLD
                     LLSGQGAGLGICTNKAQDLAELLLDALQLTHHFPAIIGGGRTPYNKPDPRPLLETVAA
                     LKGRRERAILVGDSPVDVAAARAADIPVIAMSYGYTPVPVHELGADAVADDFTQLPEI
                     ISRLVS"
     gene            complement(20053..20628)
                     /locus_tag="VGM72_12045"
     CDS             complement(20053..20628)
                     /locus_tag="VGM72_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266042.1"
                     /translation="MRRLGLGIVALLMLTAPAVAAGWQTYTFPDQGFSIESVVPLIKG
                     AGLYKGAIAGSIPTVTYTGDAENIRYKVSVIDISKRPADAVNLYLEMEFLTSLSGKVL
                     ANESVGIEPGNERHYGRQLAIQTKNGNLVRTVLVYNKGKIYATEAAILPNGDKDSFWP
                     ERFVESILFDLDGAARERDADPANFKTPAGK"
     gene            20743..21153
                     /locus_tag="VGM72_12050"
     CDS             20743..21153
                     /locus_tag="VGM72_12050"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HEY4266043.1"
                     /translation="MTTQSLSVTGILAALATMPVAMPASAHHSFAMFDAEKTITLNGT
                     VKEIEWTNPHMWLHVMAPDQSGKVQEFPLEMQGPGQSMKNGWKQDSVKPGDKVTVEMH
                     PLKTGARGGQLLTVVLPSGQKLNVGGKAPSPFGD"
     gene            21349..23331
                     /locus_tag="VGM72_12055"
     CDS             21349..23331
                     /locus_tag="VGM72_12055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4266044.1"
                     /translation="MSRRLRQLTSIRVLLPTVTGVMTLVLVTIFAIFGTHALQRRDEV
                     RRIPAIVNISYDLFAAIQDFRLERGAVNRALAGFEVPGGDERAEIAKLRTRSGKSLDS
                     ALAKLAAMNFAGIEPEIQNVQYRRDSFVALRGDIDRALEHPEIRRGDKLYSTWLDATS
                     KLVGAIDAMSARLESELSQSDFFVAEMIRVKQVVWPLRSDSGDDRLLVREAMTSGKPL
                     SSEQRRKLDLLSGRIAFAWQIIQDAGNRATTPAKLKEAIGAADKIYFTEFLTLRDHVV
                     DELSAGRSVNIDLRKWLNLSGEGRASVYAIAKTAFDLASSHAAEQAATAEREFYAALG
                     LMFFFFGVGAVTALYVIKGVVHPITEIADTMRIVADGNLTCAIPFENRADEIGLLSRG
                     LRIFRDSAIEKEQLYLAKVGAETSNRTKSEFLANMSHELRTPLNAIIGFSDVIKRSLF
                     GPISERYRDYATDIFNSGTHLLKLINEILDLSKLEAKQVELYEENIDFAILIPSCLHL
                     IEPQAESAKVQLLSAIGENVPLIRADERRMRQILFNLLSNAVKFTLEGGQIRVSASLT
                     REGVALTVTDTGIGMSAEQIPIALESFRQIDSKISRRYDGTGLGLPLTKHLVELHGGA
                     MAIESALDAGTTVTCLFPRERIIEPALNSTLARQAG"
     gene            23547..>23831
                     /locus_tag="VGM72_12060"
     CDS             23547..>23831
                     /locus_tag="VGM72_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266045.1"
                     /translation="MADVKVSEELAKKFATEKDRDTPYQRWVRAEGLDIISGHYVANL
                     RSVELNAWPRRGGRGVYINHDASRTSNDCYVCEIPPGKSLTPQRQLFEEMV"
BASE COUNT         4614 a         7547 c         7031 g         4639 t
ORIGIN      
        1 taagggcgca gcaatcacgt catggccaac tttgatcatt ccggttcccg ttccggcttc
       61 ggcgcgcagc gggcgacagt aaaatggttt aacgcgacta agggttttgg cttcgtaacc
      121 ctggatgatg gatcggatgc attctgtcac gcatcggcct tggtgaatct tccgagtgcg
      181 gaactgccgc agggcgcgac ggtgtattgc gaggtggcgc agggccagcg tgggatgcag
      241 gtcaccagcg tgcacagcgt ggatctttcc accgcagagg cacagccgtc acgcggcccc
      301 ggcgggccgc ggccgcaacg tggcccacgc gaatccggtg cgggtggcgc tatgggcgat
      361 ggccatgtga agttcttcaa cgagcagaag ggatttggct tcgtagtacc ggaatcggga
      421 ggcaccgatg tctatgttca cgcaagcgcg ctccgccgct cgggtgttcc gacactgatg
      481 ccggaacagc gggtacgctt ttcgacccgc cagggcatga agggcgtcga agtcgatcgc
      541 attgaattgg cataaccacc gcggatcgcg ttgcgagcgc ctatcgctcc cgcacggtgc
      601 cggtcttgtc caccacgaca tcccctgcct tcatcaccca gcgcacattg ttgggcacga
      661 cggaaatgtc ggcgagcgga tcgccttcaa ccgcaaccat atcggcgaga taaccgggcg
      721 cgaccgcgcc cagacttttc tccattccca gcatctctgc gggcaggatg gttgccgact
      781 gcagcgcctg ttcattggac atgcccagtt tcacgaacca ggaaagctcc tgcatgttcg
      841 tcccgaatgc ggtgtagaca gcgtcggacc cgacaagaat gcgggcgccg gcacggtacg
      901 ccttttgcgc cgtcacgaaa ttgcgctgaa tgaaattgcg caaattctcc tttgtctcgg
      961 gcgtgaattt gtacacgtcg tcggcacgat cgagataatg ctgattgtga ttgatcgtgg
     1021 gcacgtacca catcttgttc cgcaccagcg cggcgatggt cgcatcgtcc atgtcggtgg
     1081 catgttccag cgtatcggcg ccggcgcgga tcgcgtcgcg tgccgccgca gggccgtagg
     1141 aatgaacggc gactttgcgc cctgccgcat gtgccgtgtc gatgatcgct ttcatctcgt
     1201 cataggagac ggtctgctcg cccgtaagat cgtcggcgat gccggtggac gcgtagatct
     1261 tgatccaatc gacgccggac gcgagcaagg attttgtcag cttcacggct tccgcgacag
     1321 gatcggtcac gccggggtgg cgatacgtca tgtaacggat gcccgcaccg gagacgaaca
     1381 tccgcgggcc gatcaccgcg ccccgattga tgagatcgcg aagcgcaaga tcggcaccat
     1441 cgagaccgtt gagatcgcgc acggtcgtga cgcccgcctc cagcgtcttg cgtgcgttct
     1501 cctgcgcaag gaaaaccgtc accgcgacgt ggcgcggcgg ctggtgcagc ggagtcgcgc
     1561 cggtcgaacg gtcccaataa aacgtcatgt gggtgtgcga atcgatcaga ccgggaaggc
     1621 ctgtatagcg ggacagatcg acggtctccg cgccggacgg aatggtgccg tttgcggtga
     1681 cgctttggat tttgccgtcc tgcaccgcga cgaccgcatt ggcgagcgcc cggtgaccat
     1741 cccacaattt tccgaatcgg acccatttgg tcgcggcttg cgcatcgaga gaagcgcaga
     1801 gcagcagggc gagcaccatc gtaccaagcc gaaacttcag gcgcatggga acaccgctat
     1861 cgctgatcgg accgccgctc gccggcactt ccttcttgcc cttcgcgcag ttcctcggcc
     1921 tgcttgtcgg cttcggcgag cgtgctcacg cattgcgcat agaggggcgt gtcaatttcc
     1981 gctccgaaag aaagacagcg atcatgataa ccgatcatcg cgttttgcgt gatcgtttcc
     2041 atggccttgg cgatatcata attggggtct ccgggcccat cattggcgca ccccgccaaa
     2101 accaacgcga aaaaccaagg ctttcgcatt tccccctcct aaaacctgca actccgtttc
     2161 gtgtcattcc ctcgggcacg tccagtctct gccctatcca ctactccgcc gcgccgcctg
     2221 caacgacacg gcgtttcggg tcggtgacgg cgccacgcag gtcctccgga tcgccttcca
     2281 ccgcctgcag cctttcgcgc acttcggcgg cttccttttg ccggttccac atcgcggcat
     2341 aagctccgcc gcgcgcgata agctccccgt gccggccacg ttccacgatc tggccgcggt
     2401 cgagcaccag gatttcgtcc gcatcgatga tcgtggaaag ccggtgcgcg atgacaagcg
     2461 tggtgcggtt cttcgacacc agtttcagcg aagactggat ttcgcgttcg gtatgggtat
     2521 cgagcgccga ggtcgcctca tccagcaaca ggatgggcgg gttcttcagc agcgtgcgcg
     2581 cgatggcgac gcgctgcttt tcaccgcctg agagcttcaa tccgcgttcg ccgaccatgc
     2641 tgtcatagcc tgtcggaagt tcgcggatga taggatcgat ctgggcaagc cgcgcggctt
     2701 ccagaatgtc gggttcgttc gcgccgatgc gcccataggc gatattgtag cggatggtat
     2761 cgttgaaaag gacggtatcc tgcggaacga tgccgatggc cgcgcgcaag gacgcctgcg
     2821 tcacgtcgcg tatatcctga ccgtcgatgg tcacgcttcc cgattggatg tcgtagaagc
     2881 gatagagaat gcgcgagatg gtggattttc ccgcacccgt aggacccacc accgccaccg
     2941 tctttcccgc gggcacacga aacgaaacgc ctttcaggat gatgcgatcg ggttcataag
     3001 cgaagaccac attgtcgaac gcaatgtcgc catgggcgat ggcgagaggc tgcgctcctt
     3061 ctctgtcctg gatttcctgc ggccggcgca gcaggttgaa catcgtctcc atgtcgatga
     3121 gaccctggct gatgtcacga tagaccgtgc cgagcaaatt cagcggaatg aacaactgaa
     3181 tcagcaggga attgaccatc accaggtcac cgaccttcat actgccattc ttcacgccga
     3241 tggccgccag gatcatcaag gcgacaacgc cggaggccgc gatcacgcgc tggccggtat
     3301 tgagcatgga aagcgatatc tgggtgacga tggacgcgtc ggaatatttc gtcatcgcgg
     3361 cgtcgaagcg tttttcctca tgcagttccg caccaaaata tttgacggtt tcgtaattca
     3421 gaagagaatc caccgccttg ctgttcgctt cattgtccga atcgttcatt acccggcgcg
     3481 tctggattcg ccgccgcgtc accgcgaagg tgaaataggc gtagatgagg atcattgcga
     3541 ccgtcgccgt cgtgaaccag gcattgaact gggcccacag atacaggcag atcaagataa
     3601 gctgcaggcc cgtcgggaag atgttgaaga tcgcgagcgt cagcagccgg tcgaccgcct
     3661 tggtgccgcg atcgatgatg cgcgacagtc cgccggtgtg gcgctgcaga tgaaatctta
     3721 gactgagcgc gtgcagatga tgaaacgtct ccaccgccat cacgcgcagc gcgtgatagc
     3781 aaggtctcgc gaaaatcccg tcgcggatct gcgccacggc ctggctcatg atccaggcga
     3841 ccccatagct gacgatgaag gcaatcggca cgaacagggc gatgtcttcc ggacggccct
     3901 gcagaccgtc gaccacacgg cccaggaaga tcggggtggc gacggtcaca atcggcgtca
     3961 gcacgagaaa gatcagcgag acggcgacac gccatttcag gtctggccgc ccgggcggcc
     4021 agagataggg catgagctcc aacaagggct gcaagctcgg gggggcattg ccggcaccgc
     4081 tcccgccttt tagagaccgg ccgccataac cgtaagatga catgggtttt gcgcttctcc
     4141 cgaggcccgg cctcttggaa ccacaggccg tcgccgtggt ttagctttag tggcctgcgc
     4201 cttcggcatc aattgctaaa gagttcagcc gtcataattt gagcgaatcc agcatttaag
     4261 agtaatgtat gaacagaatg ccatcctcgt taacacccgc ccgtcccacc atttgcgttt
     4321 tctgcggttc ctctcatgga gccaatcccg tctacacgga ggcggcgcgc cgattcggca
     4381 cgctgatggt cgaggaaggt ttcgatctcg tcttcggcgg tggcggtgtc gggctcatgg
     4441 gagaaaccgc gttggccgtc tccaaagcgg gcggcaagat cctgggcatc attcccagct
     4501 tcctgcgtca tgtcgaaccg ccgctgcggg tcgcctcgga aatcgtcgtc accgaaagca
     4561 tgaacgaacg caaagcgcgc atgttcgagg ctgccgacgg atttgccgtg ctgcctggcg
     4621 ggttggggac gctggatgaa ttcgccgaag ccttcacggg cgcgcaattg cgcctgcacg
     4681 ccaaacccat cgtgctcgtc aatatccaga attacttcgc gcccctggtg aggctgatcg
     4741 atcatttcgt gacggaagga tttgccggcc ccggcgtgaa agatctgttc caactcgcgc
     4801 ccacggtcga agacgcgatc gcgattttcg ccgcccatga ggccaagacg atcaagcaac
     4861 cggcggaatg atcacgccgg agccccggtc cggtagagct tgtagcagat cgaatcggac
     4921 agcgcctcga acgacgcgtc cacgatattc tccgatacac cgaccgtgga ccagcgatgg
     4981 ccatggccgt cggcgctttc gatcatgacg cgcgtgacgg cctccgtgcc gcccgtgagg
     5041 atgcggacct tgtaatcgac cagccgcaaa tcctgcagat aggacgagta atggccgaga
     5101 tccttgcgca gcgcggaatc gagcgcgttc accgggccgt tgccggtgcc gacattgtgc
     5161 aggctttcgc ccctgacatt cagtctcacc accgcttcgg acatcgtgac cagctcgccc
     5221 agcgcgttgt gccggcgctc cactatcacg cggaacgaat cgacctggaa ataattgggc
     5281 acctgcccca gcgcgcggcg cgcgagcagc tcgaaggagg cctccgcgcc gtcataggca
     5341 taacctagga attcgcgcgc cttgacgtct tccaaaagac gtccgatccg cttgtcgtcc
     5401 ggcgaaacct cgatccccgc aaggacgagg cgcgccatga tgttggagcg ccccgcctga
     5461 tccgacacca ggatgtgccg ctgattgccc acgctttcgg gcgtcacatg ctcataggtc
     5521 ttgggatttt tctgcaccgc ggagacatgc aggccgccct tatgcgcgaa cgccgacggt
     5581 cccacatagg cggcgtgacg attgggcgcg cgattgagga tttcatccag cagacgcgac
     5641 gtgggggtca gccgcgtcag cgcctcggcc gtcacattgg tttggaattg cgacgcatag
     5701 ggttccttca gcatcagatt cgccaggatc gaacagagat tggcgttgcc gcaacgctcg
     5761 ccgagcccgt tcaacgtgcc ctggatttgc cgcgcgcccc cgcgcagcgc ggccaggctc
     5821 accgccacgg cttgttcggt atcgttgtgc gcgtgcaccc cgaaattggc gccgggcagc
     5881 cgcgatttca cctccgagac gatggcgtaa gcatcttcgg gaagcgtccc accattggtg
     5941 tcgcaaagga caagccagcg cgcgccggca tcgaacgccg cgcgaagggc cgcgaaggca
     6001 taatcgggat tggccttgta gccgtcgaag aaatgctcgg cgtcgaacag agcttcgcgc
     6061 ttcttcgcca ccaacgccgc gatggattgc gcgatattgt cgagattttc ttggcgaggg
     6121 atttcgaggg cgacatcgac ctggaaatcc cagctcttgc cgacaatgca aatcgcttgc
     6181 gccttcgtgg acagaagtgc ggcgagtccc ggatcattgt ccgcactgcg cccggaacgc
     6241 ttggtcatgc cgaaggcggt gaaccgcgcc tgggcaagcg ccggtggatc gttgaagaac
     6301 gcggtgtcgg tcgggttcgc gccgggccag ccgccttcga catagtcgat gccaaggcta
     6361 tccagtgcga gcgcaatggt gcgcttgtcc tcgacggaga aatcgacgcc ttgcgtctgc
     6421 gcgccatcgc gcagcgtggt gtcgaacagg tagacgcgtt cccgactcat cgcttcactt
     6481 cccaggtcgt gcctgtgggg ccatctttga gcacgatgcc tttggcgagc agctcatcgc
     6541 ggatgcggtc ggattctttg aagttctttg ccttccgcgc ttcgttgcgc gcagcgatta
     6601 attcttgaat gtaatctggc ttcggatcaa ctttcaccgt ctcatcaata agctgcaaag
     6661 tgacccctaa tcgatcaata aacgccttaa tgtttcgctt ctgaagttca actccgatct
     6721 gattttgaaa ctcagtgtca ttgttgaggt acgctgtctt taaagagtcg cccagatcgt
     6781 agttctcgaa caatttctca tgggtgagag gacctgctga gctaacgcct tggatataag
     6841 ctgcggcttt ctgcatatct atcaaaccga gaaagcgaag atttttatag aggctcgtcg
     6901 cgctttcaag atcacctctt gctgctgccg aatgctgggt ccgtaatgcg gtcaatgctt
     6961 caggcaagtt gagatcgtcc aagagcgcac ttacgaaatc cggagttggt acattcccga
     7021 cctctcgttc cttgatctgc gcgcgagata tgaagcctct aattactcca acccaagtgg
     7081 ccaattcccg tttcgcctct gcaagagact cagacgtcca attgattggc tgcctataat
     7141 gcgaacggag catcgctaat ctgatcactg aaccgctcca cgcggtgctg gcccacccgc
     7201 gcatcaactc ccgaatcgtc acgaaattgc cgagcgactt ggacatcttc tcgccttcga
     7261 cctgaaggaa gccattgtgc atccacacct tcgcgagcgc atgtccatga tgcgcgcctt
     7321 cgctttgcgc gatctcattc tcatgatggg gaaacaccag atcgatgccg ccgccatgaa
     7381 tgtcgaaagt ctcgcccaat agactctcgc tcatcgccga gcattcgatg tgccagcccg
     7441 gacgcccgcg cccccagggc gaatcccagc ccggctgttc cggcgtggac ggcttccaca
     7501 gcacgaaatc catcgggctt ttcttatagg gcgccacctc aacgcgggcg cccgccatca
     7561 tctcatccag cgaacggcgc gcgagcttgc cgtaatcggg cttggacgac acgtcataga
     7621 gcacatgccc ttcggcggcg taggcgtgcc ctgccgcgat caaccgttca ttcatcgcga
     7681 tcatcgccgc gatatgttcg gtcgcgcgcg gctgatgcgt cggtggcagg caacccaatg
     7741 ccgcaacatc atcctggaat tgctgtacgg ttcgcgcggt caactcgctc cgcgccgcct
     7801 cgaccgacag gccgcgctcg cccgcgattt cgctcgcgcg tgcgttgatc ttatcgtcca
     7861 cgtccgtgat gttacggaca taggtgacgt gctccgcccc ataaagatgg cgcagcagcc
     7921 ggaacagcac gtcgaacacg atgaccgggc gcgcattgcc gatatgggcg aaatcataga
     7981 ccgtcggtcc gcacacatac attcggacat tcttcgcatc caaggggacg aaatcttcct
     8041 tggtccgggt cagcgtgttg taaagccgaa gattcatgac agctttcata gcaacaaagt
     8101 tcgcctctcc caacgcgtga gcgaaggaga gagcaggaat gtcggaaatt tcgggcgagg
     8161 tggagtctat atctgcggca tcagccgcga atcgcgataa tccggaagat gaaaggtgcg
     8221 cgcgtctcca tggccgttcc ctagcagctt tgcttgcgcc ggtccatggc tagcgcgcat
     8281 cgcggaaggc gttggtgatg ggatagcgcc ggtcacggcc gaaattgcgc gttgtcagct
     8341 tcaccccggg cgctgcctgg cggcgcttat attcggccac atagagcaat tgctctacgc
     8401 gccgcacaat cgccggatcg taccccttgg ccaccacctc ctcgaagctt ttttcgcctt
     8461 cgaccagaag ctccagaata ctgtccagca cgtcataggg cggcagcgaa tcctcgtcct
     8521 tctgatcggc gcgcagctct gctgtcggcg gcttgtcgat cacgcgttcg ggcatcatgc
     8581 gcccctgtgg tcccagcgcg ccgttgggcc acgtctggtt gcgccagcgc gacagtgcga
     8641 acacctgggt cttgtagagg tccttcagca cattgtagcc gcccgccata tcgccataca
     8701 gcgtcgcata gcccaccgac atttcggact tgttgccggt ggtcagcacc atggggccga
     8761 atttgttgga gatcgccatc aggatcaccc cccggatgcg cgcctgaatg ttctcctccg
     8821 tcgcatcggc cacgcgaccc gcaaagatcg gcgccaacgc ctcgccaaag gcgcgcatcg
     8881 gctcttcgat aggaacgatg tcgtaggcga tgccgagcaa tttggcgacg gccgcggcgt
     8941 cctccagact gtccttcgag gtgaagcgcg agggcatcat caccgcgcgc acacgcgaag
     9001 cgcccagcgc gtccaccgca acagccgccg aaatcgcgga atcgatgccc cccgaaagcc
     9061 ccagcacgac accgggaaag cggttcttgt tcacatagtc cttcagtccc agcaccatcg
     9121 cggaatagag cgccatgttc tggtcctgca tctccgcaag cggaccttgc tcgcagaccc
     9181 agccctcgcc ttcgcgccgc cagtgcgtga tggaaagagc ctcctcccag ctcggcaatt
     9241 gcaccgcgag actgcggtcg gcattcagca caaaggagcc gccgtcgaac accagctcat
     9301 cctgaccgcc gacctggttc agataggcca gcggtaaatt gcattcgacg acgtgcgcga
     9361 cggtcacatt cagccggtga tcgcgcttca gcgcatcgaa gggcgaaccg ttcggcacca
     9421 gcaaaatctc ggtgccggtc tcgcacaggc attcgacggg gtctccgccc cagatgtcct
     9481 cgcagatggg aacgccgatc cgcacacccc ggatattgaa cggtcccggc ataggcccgg
     9541 gcgcgaagac acgcttttcg tcgaacacgc cataattggg cagatcgacc ttgaagctgg
     9601 tgccggcaac cttgcccgca tccagatagg caactgcgtt ataaagcgcg ccgtccttga
     9661 cccagggcgt gccgatcagc acggccgggc cgctatcggc ggtgtctttc gcgagattct
     9721 ccaccgcctc catggcgtcg gtctgaaagg cgggcttcag caccagatcc tcaggcggat
     9781 agccggtgat gaaaagctcg ctgaaaagga tgagatcggc gccggccgcc cgcgccttct
     9841 cccgcgcggc gcgggcgcgc gcgagattgc ccggaatgtc tcccatcacg ggattgagtt
     9901 gggcaagagc gatggcgaat gcgttgggag ctttggcgtt catgggcgct gtttagcgcc
     9961 ttatcttcgc aggcaaaagt gacgagcggc tcaagccgtg cggcgaagcg ggctgctctt
    10021 gggcttgagg atttcaccgc tgggcaccag cgcctcttcg atcagaagcg cggtttcgag
    10081 cgcgcggcag gcctcttcgg ggcgaaccaa cgtggaggcg cctgcgcggg ccgccgtcgc
    10141 gaaggccgcg accgattccc acaagggatc gcgcacttcc atattctgca gtttacgcga
    10201 ggttgtgttg gtgacttcgc gggtgaggaa gtcgatttcg atctcgccgt cgggatagac
    10261 catccgcatg aagcgccggc gcgcatcggc ggcacggttg gccaccagct cgacctccga
    10321 gccgttctcg aaaacgagat tggcggtcac ctgatccgtc aatgtcccgc acagaacgcg
    10381 gccctcggca cgcaccccgg cgatcccccc cggcaccagc cgatgaatga ggtccagatc
    10441 gtggatcatg agatcgagca cgacgctgac atcggttccg cgtccagtcc acgttccttc
    10501 gcgcacacat tcgatggaga gcggcgcatc ttcgtaatcc agcaaaccgc ttttcgcgaa
    10561 aacgaaacgt tcctgatggc cgaccgtcaa aaccagcccg cgcgccgcgg caagttcaat
    10621 cagcgcgtcg gcttcttcaa catcggtggc gatcggcttt tccaccatca catgcgcgcc
    10681 cgcgttcagg aacgccctta cgatttccgc atgcgtgctg gccggcgagc agacgctgac
    10741 gatatcgacc ttgcccaaaa gctcgcgcca atcggcgacc acgggcactt cgagctgggt
    10801 ttcgacaaaa tgccgggcct ccgcgctggg atcggccatc gcgtcaagct taacgccctg
    10861 catgcgcaga tatttggccg cgtggtgacg tccgaagact ccggccccga caatggcggc
    10921 ccgcaagact cgctgcttgg aaagctgatg aatggtcaaa aacgaacgcc ctgctgcaaa
    10981 tgcgaattca ccaagaatcc aatgcctgca aaggcactta ggcgccactc ataaaatgtt
    11041 tcgcgatatt acgccgtacc cggacttatc attaagccac tcaatccaaa cggaaatcta
    11101 agagattacg gccgtcttaa cgggcgaaaa ttggctcttg cgttcctcgc gcagattttc
    11161 cgccaccaga aaggccaggt ccagcgcctg gctcgcattg agccgcggat cgcaatgggt
    11221 gtcgtattgg cgcgcgagat cgtcttccga aatctcctgc gggccgccca ggcactccgt
    11281 cacgtccttg ccggtaagct ccagatggat gccgcccgca tgggtgcctt cggcgcggtg
    11341 cacttcgaag aaggtgcgtg tctccttcat cacccggtcg aaggggcgtg tcttgtaacc
    11401 ggtcgaggcc ttgatcgtgt tgccgtgcat gggatcgcag gaccagacga tcttcttgcc
    11461 ctcgcgggtg acagaacgca gcagcttggg gaatttctcg ccggcctggt ccgcgccaaa
    11521 gcggcagatg agtgtcagcc gcccgggaat attctccgga ttcagaatat cgatcagccg
    11581 cagaagatcg tcctcgccaa gcgatggccc gcatttcagc ccgatgggat tcttgatccc
    11641 gcggcagaat tcgaggtgcg cgccatcgtg ctgacgcgtg cggtccccga tccagagcag
    11701 atgcgcggag gtgcaatacc aatcgccgct ggtggaatcg atccgcgtca gcgcctgctc
    11761 atagcccagg aaaagggctt cgtggctggt atagaaatcc gtcgtgcgca attgcggcac
    11821 aagctcgggc gtgacaccgc aagcctccat gaagcccagc gcctcgccga tgcgcgccga
    11881 cagcgcgcgg tagcgttcgc tcgccggcga gccggcgatg aaatcgaggg tccagcggtg
    11941 gacattgtgc aaatccgcat aaccgccggt ggcgaaggcg cgcagcagat tctgcgtcgc
    12001 cgccgattga ccataggcct gcagcaaccg gtcgggatcg ggcacccgcc ccttggcgtc
    12061 gaactcgatg ctgttgatgt tgtcgccgcg ataggacggc agcgtgacgc ctttctgggt
    12121 ctcggtgtct tccgagcggg gtttggcgaa ctgccccgcg atgcgcccca ccttcaccac
    12181 cggcatggcg cccgcaaacg tcagcaccgc cgccatctgc aggatcacgc ggaaggtgtc
    12241 gcggatattg tcggggtgga attcggcgaa gctttccgcg caatcgccgc cctggagcag
    12301 gaaagccttc ccgtccgcca cctggcccaa agccgccctc agattgcggg cttccccggc
    12361 gaacaccaga ggcgggtagc tcttgagccg cgcctcgacg gccgcgagcg ccgcggcgtc
    12421 gggatattgc ggcacttgtt tgatgggcat cccccgccag gagctggggg tccagggctt
    12481 tgccacgaag gcctcctttg attcaggctg gtggatatag gcgatggcgg ggtacttcgc
    12541 cagtcggatt aatcatgacg tgccttgcat taagtacgtg ccggttcacg gatttggccg
    12601 gatatcctcg ctcaggcaca tccctggggg ccaatttatc cacagccctc cgagactggg
    12661 ggcagttaag cggccaatac cccatcggtc caggcggcaa acgcgtccag catctcgttt
    12721 cgaacttggg catcgttgcg gccgcttttc gcccggacat ggaaggaatg atccgcgtcc
    12781 ttgaacagtt tcagattggc gagacggcca agcgatgcgc acagcggctc aagctccgcg
    12841 agctcggcca gggcatcgcg ggtcccttgc aggaacagca ttggaatatg cacgtcgaac
    12901 agatggttgc ctcggtcacg cgagggctta cccaccggat gcagaggaaa gcccagaaaa
    12961 gcgaggcctc gaacgccgct caagggtgcg gcggcttgag cctgggatgt catgcggcca
    13021 ccgaaagact tgccgcccgc aagcaacggc acaccgggca aaaggcgccc ggcctcggct
    13081 accgccgcgc acgcggtggc ttgcgccaat ttgggggcgt cgggccgctt tcctcccgct
    13141 tccatataag ggaattggta gcgcagagag gcgatatcgc gctggtcaag gccttcggcc
    13201 attgcggcga ggaagggatg ctccataccg gcgccggcac catgggccag cacatagcag
    13261 gcgcgcgcag ccttcgggat ttgcagaaga ccagagaccc tacctccctc tacagcaaat
    13321 gtaacgcgct tggcctgcgc ttcattcatg ccgacatctt accgcaaagc ttgattcccc
    13381 gcccccacaa gaccatctaa gtctggctca gaccgccatg caggagattt tcgatgtgcc
    13441 gcaacatccg cacgttgttc aatttcgcgc cacccgcgag cgaaggggaa atccgcgcct
    13501 cggcgctgca gttcgtgcgc aagctgagtg gtttcgccgc gccgtccaaa tcgaatgatg
    13561 cagccttcca gcgcgcggtg gacgaaatat ccgaagtcgt tcatcgcctg ttcgaatcga
    13621 tggaaaccaa cagcccgccg aaaaatcgcg agatcgaggc agagaaggcc cgcgagcgcg
    13681 caaagctgcg cttcgcgtag ctcccgactt attgtctcga agactcctgg tacagatatc
    13741 cacccagccg attgcactcc aaattcgggc ccagtgctcc cctttgcaaa cgatgccggt
    13801 atcttcatca tcattcggac ggtgagcgcg ttgccgtctt ggttcgcatc gtcaccaatt
    13861 cctcggccat tgtcggatga accgcgaccg tcgcgtcgaa ttgcgccttg gtcgccccca
    13921 tcttgagtgc aatcgcaaca agttgggcca tctcgcctgc atccgggctg aaaacgtggc
    13981 agccgagaac tttgtctgtt tttgcatcca ctacgagctt catcatggaa cgttcgtgcc
    14041 ggcccgacag cgtgtttttc agcggtctga aattcgtctt gtagatgtcg atggtgtggc
    14101 cgcgtttcag ggctcgttcc tcgctcagcc cgacggtgcc aatttcgggg cgcgtgaaca
    14161 ccgccgttgc caccaattca tgatccggcg cggaaggctt ttccccgaat acggtctcga
    14221 caaaacacat cgcttcgtga agcgccacgg gcgtcaattc gagacgcccg gtcacatctc
    14281 cgatcgcata aatgttggga accgcggtcc gtgaataacg gtcgacatcg atatgccccg
    14341 aagcaatcgt ggtgactccg gccgcctcca gtcccagccc catgacattg ggccggcgcc
    14401 caaccgcaag catcacctga tccgcgtcga tgatctcgcc cgctgtcgtc gtcgcgcgca
    14461 acgtcttgcc cacgcgttcg caggccgcga gcttggtgtc aagcagaagg tgaatcccct
    14521 tgttgcgcat cgaagcttgc aggctatcgc gcagatcctc gtcgaaaccg cgcagcactt
    14581 ttggcccgcg acagacgagc gtcacctccg cgccgagccc gtggaagatg tgcgcgaatt
    14641 ccatcgcgat atagccgctg ccgacaatca cgatggaacc gggaaatttt tccaggtgaa
    14701 atgcttcatt cgacgtgatg caaagctccg cgcccaccat ctcggtctgc cgcgaaggat
    14761 accctccggt cgcgatgagg atggtttgcg cggtgacgtc acgagccgcc ttcttcaagt
    14821 gaatggtgtg cgtgtccttg aaaatcgcgc gatcctcgaa gatcgcagcg ccggcatttt
    14881 ccagcacgct gcgatagacc ccggagatgc gcgcgatgtc cttgtccttg ttcgcgatca
    14941 gcgctggcca gtcgaagcgc gcaccgtcca tcgtccagcc gaagccgcgc gcgtcctcga
    15001 attcctccgc gaagctgctc gcatagacaa acagcttctt gggaacgcag ccgcgcacga
    15061 cgcaggtgcc gccgaagctg tcctcttcgg cgaccgccac gcgcgcgccc ctctgcgccg
    15121 cgagacgccc cgcccggacg ccgccggacc ccgccccgat gacgaacaga tcgaaatcat
    15181 aggtgtcgga cgccatgcat cctttcagca tgccccgtgc gtgcgggcaa gctcagcgtc
    15241 gggaaacacg ctgtctgcgc tcgataacgc gcacaccacc ggcgcctgcg atgacgagcg
    15301 ccgaagccat attcacgaac agcccatgtt cgaccacgcc cacaatggcc gagagcgacg
    15361 cggccagagc cttcgcgtcg gcaatttcac caaacgggca atcgtagatc acattgccgc
    15421 tgtcggtgtg gaaagcctgg ccgtcctttt tacgcagcgc aggcgacaaa ttcgcgtagc
    15481 ccagttttgc ggcggccgca gaaatccggg cgcacgtcga gccgtgtccg aaggcaacga
    15541 cctccaccgg cagagcaaaa gcgccaagcc gatcaaccaa tttgctgtca tccgcgatga
    15601 ccaccatgcg cttggccgag gccgccacga tcttttcgcg caatagcgcc ccgcccccgc
    15661 ccttgatgag atcgagattg ccatcggcct cgtccgcgcc gtcgatcacg agatccagtg
    15721 tgcccagcgc gtccagttcg gccatcgcga tgccaagttt gcggcccaat gcggcggtgc
    15781 gttccgaggt cggcgtgccg gtgattttca acccggcgcg catccgttcg gccagaagct
    15841 ccaggaacaa ctccgccgtg gtgccggtcc ccagccccag tttcatcccg tcttcgacaa
    15901 acgcgagcgc acgcgccgcc gcctcgcgtt tgagccgttg ctgttcgcca tcgttcatgg
    15961 cggaactaga gacgaaactc caaagcctgt cgaggcgaaa ggatgccgtt cagcggcgcg
    16021 gcttactcca ccgtcaccga tttcgccaga ttgcgcggct gatcgacatc ggtccccttt
    16081 gccacggccg tgtgataggc gagcagctgg atggggagcg cataaagcag cggtgagatg
    16141 agggcatcgg tttccggcac ttcgatggtt gcccaggtac ggtccgcacc ctgatcgagg
    16201 cccttgcggt cggaaatcag caccaccttg ccgccgcgcg ccatcacctc ctgcatgtta
    16261 gaaatcgatt tctcgaacag ctcgtcatgg ggcgcgatca caatcaccgg cacattgcca
    16321 tcgatcagcg cgatggggcc gtgcttcatc tcgccggagg catagccctc ggcatggatg
    16381 taggaaatct ccttcagctt cagcgcgcct tcgagtgcgg tcggaaaatg cacgccgcgt
    16441 ccgagataga gcacgtcctg cgccttggcg atctccaggc ccaacgcttc gatcttcgct
    16501 tcctgggctt ggacgagcag cgacaccatg tgccggggaa cttccagcaa caggccgcag
    16561 aggcgttttt cctcggcctc atcgatgtga ccgcgggcgc gcccggcagc gatcgcaagc
    16621 gcaaccaaca cagccaactg gcaggtgtag gccttggtcg aagcaacgcc gatctccggc
    16681 ccggcatagg tgggccatac catgtcggca tcgcgtgcga tggagctttc cggcacgttg
    16741 acgatcgcag cgaccgtctg gcccgcggcc ttggcatcgc gcatggcggc cagcgtgtcc
    16801 gccgtctcac ccgactgcga gacgtaaagg cacagacctc cctcggaata aaccggattg
    16861 cggtagcgca actccgacgc cacatcgaca tccaccggca tccgcgccag tttctcgaac
    16921 cagtattttc cgaccatagc ggcatagaag gcggtgccgc atgccgaaat ggtcagccgt
    16981 ggcgcattgc ggaacaaggc cgcggcttcg ggcgcaaggt ccaccttggg cccggacgca
    17041 tcgacgtagc gcgcgatggt atggcccacc acctcaggct gttcgtggat ttccttggcc
    17101 atgaaatgac gccggttgcc cttgtcgacc atcgcgcccg acggcggaat gatgtggatc
    17161 ggtcgggcca cccatttgcc ggcggaatcg tagatcatga catcggcccc gcgcaccacc
    17221 gccacgtcgc cgtcttccag ataggcaaca cggttggtga aaggcgcaag cgagaacgca
    17281 tccgagccga gataggcttc ggtctggccg tagccgaccg cgagcgggct gccccggcgc
    17341 gcggcgatca gcagccgttc ttcccctttg aagatcatga ccatcgcgaa cgcgccttcg
    17401 agtcgtttga cagccgtctt ggcggcggct tccggcgaca gtccctcgtc cagatattgc
    17461 gtgatcagat ggacggcgac ctcggtatcg gtttcggatt cgaaccgatg tccggcggcg
    17521 cgcaattctt ccctgagctc gcggaaattc tcgatgatgc cattgtgcac cagggccacg
    17581 cgcgatgagg catggggatg ggcattcagc tccgtcgccg ccccgtgggt tgcccagcgc
    17641 gtatggccga tgccggtgaa gcccggcagc ggattttccg ccagcaaccc caccaattga
    17701 ttcagcttgc ccgggctgcg gcggcgatcg agacggccct ggttcagcgt cgcgacgccg
    17761 gccgaatcat aaccgcgata ttccagacgc ttcagtgcat ccacgatgag cggggccaca
    17821 tcgcgcgttc cggcaatgcc gatgattcca cacattcaga cgttcctatc tttcttggcc
    17881 atatctttct tggccatatc tctcttggct ttgttcctgg cgcggatttg cacggcacgg
    17941 ccgggcttgg tttcctgacg tccccgggcg accgccagtg cgttcggtgc gacattctcg
    18001 gtgatcacgc ttcccgccgc gacgatggcc ccctcgccga tctcgactgg tgcaaccaaa
    18061 gcggcgttgg acccgatgaa tgcgccggcg ccgatttcgg tacggctctt ggagaaaccg
    18121 tcataattgc aggtaatggt gcccgcgccg atattggcct tctcgccgac atgagaatcc
    18181 cccagatagg tgagatgatg cgccttcacg cccttttcga gatgggcgtt cttcagttcg
    18241 acgaaatttc cgatatcgac atcggcgtcc aatttcgcgc ccccgcgcaa gcgggcaaag
    18301 ggaccgacga tcgcgcgctc gccgattgca ctgttctcga tgtggcagaa ggcgcggatg
    18361 tcggcgccgc gtttgatcgt cacccccggt ccgaacacca catattggcc gatgaccaca
    18421 tcgggctcga tgatcgtgtc ggcggacagg aacaccgttt cgggcgccgt catgccgacg
    18481 cccgcatcca tcagttgcgc gcgcgcgcgc gcctggaatg cggcttcgcc ctgcgccagt
    18541 tgtgcgcgcg agttcacccc cagcatttcg ccttccggcc cctccacgac cgcgcaacga
    18601 agaccgcgcg cctgcgcgat gcctatcacg tcggtcagat aaaactcgct ctgggcgttg
    18661 cggtttccga cccggtgcag cagctcgaaa atgagccgcg cacggcccgc cagcattccg
    18721 gcgaagcaca aatcgatgcg ccgctcgtct tcgctcgcat ctttctgctc cacgatgcgg
    18781 atcagcgagt cgccgtcggt caccatgcgt ccatagcctg cggggtcagc cgcgcgaaag
    18841 ccgagcgcgg atagatcgcc acccgcgccc agttttgcga tcaagcggct cagtgtcgcc
    18901 gccgtcaaca agggcgcatc gccgaacagc accagcaggt tcccgtcgaa accggcaagg
    18961 gcgttctcgg ctgcgagcac cgcatggccg gtgccgagct gacgctcctg caccacgctt
    19021 gtcccgcccc attggccggc gagcgcgacg acggcctcgg ctccgggcgc ggtgacggcc
    19081 acgatttgcg ccgcacccat cgcctgggca accgcgagca catggcccag catcgggcgt
    19141 ccgccaatcg gatgaaggac cttgggcagc gccgatttca tgcgcgtgcc ctgaccggcg
    19201 gcaagaacaa tgacagctaa agcaggatga ttcatgcgcg caatcgggtc caatacagcg
    19261 ttgttgaacg tcactaccgt ctaaacttaa caaggggaaa ttgacgtttt attgcttaat
    19321 ctttcttcgt gacgctttct gggctaagtc ccgttcccat cggcactttt gccggatcgg
    19381 acaggaccga ccctattccc atgccggata ccccgctcgc cattgttttc gacctcgacg
    19441 gaactctggt cgacacagcg ccagaccttc tgagcgcgct caacgccgtg ctgacagccg
    19501 aaaatcacga gcccgtcgtt ccgtcggacc tccgccatat cgtaggccac ggagcgcgtg
    19561 ccatgttcga gcatgcgcta ctgcgcaccg gcacgccagt gtccgcggcc cggctgaata
    19621 ccctcaccga aaagttcctg gcctattatc gtgccaatat cgcgtgcggc agcaggcttt
    19681 ttccaggcgt tccggaaacc ctcgatctcc tctccggtca aggagccggc ttgggcatct
    19741 gcacgaacaa ggcacaggac ctcgcggagt tgctcctgga cgcgctccag ctcacccacc
    19801 atttccctgc gatcatcggc ggcggccgca cgccctacaa caagccggat ccgcgccctt
    19861 tgctcgaaac ggtggccgcg ctgaagggcc gccgcgagcg cgccatactg gtgggcgatt
    19921 cgccggtgga cgtggcggcc gcgcgcgccg cggacattcc cgtcatcgcc atgagctatg
    19981 gctacacgcc cgtaccggtg cacgaacttg gcgccgatgc ggtggccgac gatttcacgc
    20041 aattgcccga aattatttcc cggctggtgt cttgaaattg gcggggtcgg cgtcgcgttc
    20101 gcgcgcggca ccgtcgagat cgaacaaaat cgattccacg aaccgttccg gccaaaagga
    20161 atccttgtcg ccgttcggta gaatggcggc ctccgtcgcg tagatcttgc ccttgttgta
    20221 gacgagcacg gtgcggacga gattgccgtt cttggtctgg atcgcgagct ggcggccgta
    20281 gtggcgctcg ttacccggct cgataccgac gctttcattg gccagcacct tgccgctcaa
    20341 cgaggtcagg aattccatct cgaggtagag gttgaccgca tcggcggggc gtttcgagat
    20401 gtcgatgacg ctcaccttgt agcggatgtt ctccgcatcg ccggtatagg tgacggtggg
    20461 aatggacccg gcgatggcgc ccttataaag gcccgctccc ttgatcaagg gcacgacgct
    20521 ctcgatcgag aagccctggt cgggaaatgt gtaggtctgc catcccgcgg ccacggccgg
    20581 cgcggtcagc atcagcaacg ccacaattcc cagacccagt cgacgcatga actcctcctt
    20641 gccgcccggc ttctatctgc cgccgccggc gatggcgctt gcgcacgcca tattatagta
    20701 tccgcaggaa cagggccaat cggcggccgc aggggggatt cgatgacgac ccagtctttg
    20761 tccgttaccg gaatcctggc tgcgctggcg acgatgccgg tggcgatgcc ggcatcggcc
    20821 catcattcct tcgccatgtt cgatgcggaa aagacgatca cgctgaacgg aacggtcaag
    20881 gaaatcgagt ggaccaatcc gcatatgtgg ctccatgtga tggccccgga tcaaagcggc
    20941 aaagttcagg aatttccgct cgaaatgcag ggtccgggcc agagcatgaa aaatggctgg
    21001 aaacaggatt cggtcaaacc gggcgacaag gtcaccgtcg agatgcaccc cctgaaaacg
    21061 ggcgcgcgcg gcggacaatt gttgaccgtg gtgttgcctt ccggccaaaa gctcaatgtc
    21121 gggggcaaag caccgagccc attcggagac tgattcgctc cctccgtacc catcgtgaat
    21181 gcgctcacgc gcctgtggca agcccgcccg catcttgccg cggctgatcc tattggttga
    21241 ttttcctgcg ttttacagac cttcagcagg aattaatgca atcctgccat cctcaacctc
    21301 cagtatggtc aaggcccgaa gacgggccat tcgcaggcga tcccacgcgt gtcacggcga
    21361 ttgagacaac tgacgtccat ccgggtcctt ctgcccacgg tcaccggcgt gatgaccctc
    21421 gtcctcgtta ccattttcgc gatttttggg acgcatgcgc tgcaacgccg cgatgaagtg
    21481 cggcgtatcc ccgccatcgt caacatttcc tacgacctct tcgccgcgat ccaggatttt
    21541 cgcctggagc gcggtgcggt gaatcgcgct cttgccggat ttgaagttcc gggcggcgac
    21601 gagcgggccg agattgcgaa attgcgcaca cgatccggca agtccctgga ttcggcgctg
    21661 gccaaactcg cggcgatgaa ttttgcgggg atcgaacctg agatccaaaa tgtgcagtac
    21721 agacgcgata gcttcgtcgc gttgcgcggc gatatcgacc gggcgctcga acatcccgag
    21781 atccgccggg gcgataaact gtattcgacg tggctcgatg cgacctccaa actggtaggc
    21841 gcgatcgacg cgatgtccgc gcgtctggaa agcgaactga gccaaagcga ttttttcgtc
    21901 gccgaaatga tccgcgtaaa acaggtcgtc tggccgctgc ggtccgattc gggcgacgac
    21961 cgcttgctgg tgcgcgaagc catgaccagc ggcaagccgc tctccagcga acagcgccgc
    22021 aagctcgacc tgctgtccgg acgcatcgca ttcgcctggc aaattatcca ggatgccggc
    22081 aaccgcgcca ccacgcccgc caaactgaaa gaagccatcg gcgcggcgga caaaatctat
    22141 ttcacggaat tcctcaccct gcgcgatcat gtggtcgatg aactctcggc cgggcggagc
    22201 gtgaatatcg acctgcgcaa atggcttaat ttgtccggcg aaggccgggc cagcgtgtat
    22261 gccatcgcca agacggcgtt cgacctcgcc agcagtcacg ccgccgagca agccgcgacg
    22321 gccgaacggg aattctatgc agcactcggc ctcatgtttt tcttcttcgg tgtcggagcg
    22381 gtgaccgcgc tgtacgtgat caagggcgtg gtccatccga tcaccgagat cgcagacacc
    22441 atgcgcatcg tcgcggacgg caatcttacc tgcgcgattc cgttcgagaa tcgtgccgac
    22501 gagatcggtc tcttgtcgcg cggacttcgc attttccgcg acagcgctat cgaaaaagag
    22561 caattgtatc ttgccaaggt gggtgcggag acatcgaacc gcaccaagtc ggagttcctg
    22621 gccaatatga gccacgagct gcgtacaccc ctgaacgcca tcatcggatt ctccgatgtc
    22681 attaaacgaa gcctgttcgg tccgatcagc gagcgatatc gcgactacgc gacggacatc
    22741 ttcaacagcg gaacgcatct gctgaagctg atcaacgaaa tcctggacct ctcgaagctg
    22801 gaagccaaac aggttgagct ctacgaggag aacatcgatt ttgccatcct cattccatcc
    22861 tgcctgcacc tcatcgagcc gcaggcggaa agcgcgaaag tgcaattgct gagtgcgatc
    22921 ggagaaaacg ttcccctgat tcgggccgat gaacgacgga tgcggcagat cctgttcaat
    22981 cttctttcca atgccgtgaa attcacgctc gagggcggcc agattcgcgt ctcggcatcc
    23041 ctcacccgcg agggcgtggc gctcaccgtc accgataccg gcattggaat gtcggctgaa
    23101 cagattccga ttgcgctcga gtcgttccgc cagatcgaca gcaagatcag ccggcgctat
    23161 gacggaaccg gccttggcct tccgctgacc aagcatcttg tggaattgca tggcggtgca
    23221 atggcgatag aaagcgcgct cgacgccggg accaccgtga cgtgtctttt tccgcgcgaa
    23281 cgcatcattg aaccggcgct gaattccaca ctggcaaggc aagccggcta ggcgaattcg
    23341 tgccctgcgt cctgcggttt caatatccgc gccctatcaa ccactaattt ccagcctcgc
    23401 cgttggcggg gcgtcgagcc atacgctccg ccatgccgcc ggacttggac tcggtgagcc
    23461 acatgccggc gaccgggccg ggtggaattc gtctgcgcct tggcgtagga ggctatggac
    23521 gcgcatcgaa ttcgtgagga gcagccatgg ccgatgtgaa agtttccgag gaactggcaa
    23581 agaaattcgc caccgaaaag gaccgcgata ccccgtatca gcgctgggtc cgcgccgagg
    23641 gactggacat catcagcggc cattacgtgg cgaacctgcg cagcgtagag ctgaatgcct
    23701 ggccgcggcg tggcggccgt ggcgtctaca tcaatcacga tgcctcgcgc acctcgaacg
    23761 attgttatgt ctgtgaaatc ccccccggca agagtctcac gccgcagcgc caactcttcg
    23821 aggagatggt g
//
LOCUS       DASYEG010000284        27930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_109767, whole genome shotgun sequence.
ACCESSION   DASYEG010000284 DASYEG010000000
VERSION     DASYEG010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 27930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27930
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109767"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VGM72_12065"
     CDS             <1..861
                     /locus_tag="VGM72_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266046.1"
                     /translation="QSFDVYTNQENFFTVNFPGTPTATQVTHKTLKGTELTAHVFTAT
                     VPPGARTSGTYSVTVVDFAGALDEMSTAVEHAADAIRAKGAIKYDAIENLDLHASRRL
                     TVETATDRILAEILFAPNNRLYIVQAENSLTQTPPAQFQASLQIIDNAGARIRTRTLL
                     GLPEGSKQPLNAGGIADEPDTVAALMSGSWRSAGGTCEAPYFKSGMRSKNSRGEAVMA
                     GTITNGGTTINGMLIVEAARAGQFVDPVSFQALMLFDPMGDKLGISSIGGAAASWPDV
                     TLELCPGSRT"
     gene            983..1930
                     /locus_tag="VGM72_12070"
     CDS             983..1930
                     /locus_tag="VGM72_12070"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY4266047.1"
                     /translation="MRNWFAASLLILAPLMAAAAEAPPEWAYPAAPAGFQPPPDDGQP
                     KHVAGSTKTFTEKDVNNIHNPPDWFPGEHPPLPDLVAHGKEGVNACAQCHLATGYGHP
                     ESANLSGLPADYVEEQLNAFKSGDRRSSLPGRSANMINFAKAMNESDMKQAAAYFSSI
                     KPVVWTKVKEAATVPKTYVGEGNMRFVSTGTQTEAIGKRIIEVPEEEEGAKVRDPHSP
                     FVAYVPVGSIEAGETLATTGGGGKTIQCSVCHGADFKGIGNVPRLAGRSAIYIFRQLN
                     DIQKGTRHGNAVALMQPVVAKLSEDDMIALAAFMASRTP"
     gene            2028..2981
                     /locus_tag="VGM72_12075"
     CDS             2028..2981
                     /locus_tag="VGM72_12075"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY4266048.1"
                     /translation="MRKLLLISAIAMLPAGAFGAESALSWAYPVAPQGLPRPDPNKTF
                     QAKGAPADMKLTAAQINNAFGPPDWFPNEHPVMPSIVKNGREPHVRACMLCHLPNGNG
                     HPESASVSGLTATYITEQMHEFRAGNRMNNRAPVMIEMAKDITEPELKEAAEYFASIP
                     KAEQKWIRVVEGKTAPKNHVGNGGMRFFDEGGGTVAVAPNMIYEVAEDGEAAELRDQH
                     YGFVDYVPEGSIKKGEILATTGGGGKTIQCAVCHGADFKGIGDVPRLAGRGAYYLIRQ
                     LNDIKMGTRKGNAVALMKPVVEKLSDEDMVDLVAFMASRQP"
     gene            3055..3480
                     /locus_tag="VGM72_12080"
     CDS             3055..3480
                     /locus_tag="VGM72_12080"
                     /inference="COORDINATES: protein motif:HMM:NF019017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 domain-containing protein"
                     /protein_id="HEY4266049.1"
                     /translation="MTVHLIKLCVGADSIEDLADWQKKRSAERTKKNENGDILHITRM
                     TPKRGDELLKGGSLYWVIKGQIAVRQKLLALKPVVREGVPHCALVLDRSLVPTVRRTH
                     RAFQGWRYFDPKDAPRDLKASDKSIPEGLRAELTQLGLL"
     gene            complement(3477..4334)
                     /gene="panC"
                     /locus_tag="VGM72_12085"
     CDS             complement(3477..4334)
                     /gene="panC"
                     /locus_tag="VGM72_12085"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY4266050.1"
                     /translation="MNSSKRIETASDAVELRLRIRLWRNKGARIALVPTMGALHDGHL
                     ALVREAKARADKIVVSIFVNPAQFAPHEDFARYPRDMTKDRQFLETAGDADLIYAPNA
                     KDMYPDGFATRIVMDGPAAGLESDFRPHFFGGVAMIVTKLFVQCAPDIAIFGEKDYQQ
                     LLVVKRLVKDLDLPLGIVGVATLRERDGLALSSRNVYLNAERRSAAAHLNQVLRAVAE
                     RAKSGVSIPQAEAEGGAALLNAGFDRVDYVALRDAETLAPLRAISGPARVLAAARIGN
                     VRLIDNMAV"
     gene            4414..7545
                     /gene="putA"
                     /locus_tag="VGM72_12090"
     CDS             4414..7545
                     /gene="putA"
                     /locus_tag="VGM72_12090"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF008869.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEY4266051.1"
                     /translation="MENLRQTISAAYLADEDAVLESLIAKAKMTPAEAGTAEALARNL
                     VSQLRGGRLRHSGVDAFTREYALSSEEGVMLMCLAEALLRVPDAETQDRLIRDKIAGH
                     SWDRHLGHSESLFVNASTWALMFSGRLVETGDASRWDFEAIWHRIAARLGEPVIRQAV
                     LSAVRFLGRHFVLGRNMEEALKEARPYAERGHRFSFDMLGEAAVTRAGAGRYLEKYRE
                     AVRAIARAWPQRSVASIERPGISIKLTALHPRFEYAKRHEVMLELLPPLTRLCAEARE
                     ANLPVTIDAEESERLDLTLDIFEALGDEESLRNWDGLGLAVQAYQKRALPVIAWLIDL
                     ARHRRRIIPVRLVKGAYWDSEIKRGQELGISDYPVFTRKIGTDTSYLACARALLSAPN
                     NIFPQFATHNAHTLAAVQAFAGSSRKFEFQRLHGMGETLYEIYEEIMRPAHASATRIY
                     APVGNHEDLLAYLVRRLLENGANTSFVNRLANDKAPLEDMIADPVERLIATAPKRNPR
                     IAKPADIYPGRMNSHGFVLSDPAESSRIISGMRRAIDMGPHTANPILDGVEHIRSAKP
                     VRDPADRGREIGLVSEAADEDVRTALESAATAQDSWDRQGGAFRAAILERAAGLFEDR
                     RALLMALIVREAGRTVANALSEWREAVDFLRYYAKEARRHFEGALPLPGVTGEDNRLA
                     LRGRGVFACIAPWNFPLSIFTGQIAGALASGNSVLAKPAEQSPLIGAAAVRLLHEAGV
                     PGEVLHFLPGDGARIAKTMLADPRLAGVAFTGSTETAIAINRALAARDGPIAALIAET
                     GGLNAMIVDSTALPEQVARDVLISAFDSAGQRCSSLRVLFLQEDVADRMLEQILGAME
                     MLRLGDPFDLATDIGPVIDEEAQAALAAHADRMTKDATLLKKLTVDPALSRGVFFAPH
                     VFAIESMNALKREVFGPILHVVRFSGDRLDGVCDAINASLYGLTLGIHSRIDKTAEFV
                     RQRTRVGNIYVNRNQIGAAVGAQPFGGERLSGTGPKAGGPHYLPRFAVERTLTINSAA
                     AGGNASLLTLDEA"
     gene            7625..8494
                     /locus_tag="VGM72_12095"
     CDS             7625..8494
                     /locus_tag="VGM72_12095"
                     /inference="COORDINATES: protein motif:HMM:NF020704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HEY4266052.1"
                     /translation="MMMSTAGDRPAIADAPAKRVHVIVFGNEKGGTGKTTTAMHVAVA
                     LLRMGMNVSAIDLDSRQRTFARYIENRRDFSARKGHTLRSPDVQVVERAVSSADAVAD
                     ESTRLAEALTRAKEGADFVIIDTPGNDTLLSRLGHAAADTLVTPMNDSFLDFDLLAKL
                     DPDSLEIKSPSLYAEFVWDCRKRRLIASRANLDWVVMRNRVSATEARNKRKVATAVER
                     VSSRIGCRIAPGFSERVIFRELFPLGLTMLDLPLPGLAMPLTMSHIAARQEVRELLST
                     LRLPGVEKASGTN"
     gene            8510..8986
                     /locus_tag="VGM72_12100"
     CDS             8510..8986
                     /locus_tag="VGM72_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEY4266053.1"
                     /translation="MPALVAGVVLLGLLLLMARGYTSANARSLAKGLRFSGGIILALL
                     TVALAITGRVAFAFLAAGGAWYLLFGSPPPWQRTAYGAGGPGGSQNRSRSSPPQTGAM
                     SRVEALKVLGLEEGASEQDIRAAHRRLILQTHPDKGGTSYLAAKINEAKDVLLRRR"
     gene            complement(8990..10552)
                     /locus_tag="VGM72_12105"
     CDS             complement(8990..10552)
                     /locus_tag="VGM72_12105"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY4266054.1"
                     /translation="MPAPEKDAAIVIDGATGRLLYARNPDAIRYPASLTKMMTLYLLF
                     EALDQGKVRLDTPLITSVHASEQEPTKLAMGPGAAIPVETAIKALTVLSANDVAVVIA
                     EALGGTEDAFGVMMTQKAHELGMAHTNFHNASGLPDLQQLTTARDMALLARHLAYDFP
                     QYYHYFSAPGFSYGNRSYATHDNLLAAFGGTDGIKTGYTQLSGFNLVTSVVRNDKHVI
                     GVVLGGPTAAVRDREMMRLLSAAFEAAKSDPDMLAEIDVPWKGGKMPGPDLFKAIQGE
                     DNPLLLAALEPKSKAKSTAKIPPVLVADAKKVPGAGSGLTVVPISKPASETYPETAAP
                     VLSHETVLARETDVIEAPLGKVRTPLQFDPASAQQIADASVPLAQGDGGTDADATELP
                     NPAPVIAWAANPVPAKTLHAVPMPAAPVLANLTSSSSTLASNATVKRWAVQIGAFASE
                     TLARAKLAVFARSGFDVIGQAQKLVIPFTSGGRIMYRARLGMFAEGQARDICKRMEQR
                     GQSCFVAAADAG"
     gene            10978..11418
                     /gene="phaP"
                     /locus_tag="VGM72_12110"
     CDS             10978..11418
                     /gene="phaP"
                     /locus_tag="VGM72_12110"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Members of this family are phasins (small proteins
                     associated with inclusions such as PHA granules). Note
                     that several different families of phasins have been named
                     PhaP despite very little sequence similarity to each
                     other; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01841 family phasin"
                     /protein_id="HEY4266055.1"
                     /translation="MATKKSSTPGTEPIETALLTGAEAMKDGFEKAVKGYDQIVSFGK
                     DNAEAVLKSANAAGKGIETINSEVFAYVRKSAEDSVTAAKAVLSSKTVEEAFQLQSEF
                     GKAMFETYVDQLAKFGDMALATARHTAEPLQARVSAFSELVRAA"
     gene            11544..11933
                     /gene="clpS"
                     /locus_tag="VGM72_12115"
     CDS             11544..11933
                     /gene="clpS"
                     /locus_tag="VGM72_12115"
                     /inference="COORDINATES: protein motif:HMM:NF000669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEY4266056.1"
                     /translation="MNEGHFDKIFRPRNAGPTRGPGQGDGDSGDRGIGTGVVTKTRAK
                     TKKPSLYKVLILNDDYTPMEFVVHVLERFFAKQREEAVQIMLHVHRQGVGICGVYTFE
                     VAETKVAQVIELARRHQHPLQCTMEKE"
     gene            11943..14261
                     /gene="clpA"
                     /locus_tag="VGM72_12120"
     CDS             11943..14261
                     /gene="clpA"
                     /locus_tag="VGM72_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEY4266057.1"
                     /translation="MPSLSRSLEQALHRAIRLASERHHEYATLEHLLLALVDDSDAAQ
                     VMRACNVDLDALRAGLAKYVDEDLATLTIEDGEDAKPTTGFQRVVQRAVLHVQNSGRD
                     EVTGANVLVALFSERESHAVYFLQEQNMSRLDAVNYMSHGIAKRPGMSQPKAARGTDE
                     EEESGEKTAKQGTDALEAYCVNLNDKARKGRIDPLIGREPEVERTIQILCRRSKNNPL
                     FVGDPGVGKTAIAEGLARKIVKGEVPDVLKACTIYSLDMGALIAGTRYRGDFEERVKS
                     VVKELEALKGAILFIDEIHTVIGAGATSGGAMDASNLLKPALQAGTLRCMGSTTYKEY
                     RQYFEKDRALVRRFQKIDVAEPTVPDAIKILKGLKPYFEDYHKVRYTNDAIKAAVELA
                     AKYINDRKLPDKAIDVIDEVGASQMLLAENKRKKVIGVREVEDVVAKIARIPPKSVSK
                     TDAEALRSLEDDLKRVVYGQDSAIHALTSAIKLARAGLRQAEKPIGCYLFSGPTGVGK
                     TEVAKQLARVMGVELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDGIDQHPHC
                     VLLLDEIEKAHMDLFNILLQVMDHGKLTDHNGKKIDFRNVVLIMTTNAGASDAARESI
                     GFGRGKREGEDEEAIKRLFTPEFRNRLDAVIAFAPLTRATIDRVVEKFVLELEAQLID
                     RDVTFDLTPEATRWLGEKGYDDAFGARPLARVIQENIKKPLADEILFGKLKNGGTVRV
                     LLDRENDKLAFEFITDQKPASSEGKKKPKEEV"
     gene            14279..14671
                     /locus_tag="VGM72_12125"
     CDS             14279..14671
                     /locus_tag="VGM72_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HEY4266058.1"
                     /translation="MAMQNLRAGMGAIACGAALVFSALPAAAHHGWGGNVDQVTDMTG
                     VVVSGVSLAGPHGTMKVRTTDGHVWDITLAPPFRTSEAGLKQGTIPVGATVTVRGNRN
                     SDPNRFEMKTIRVTYAGKQYDTYPERES"
     gene            14707..15180
                     /locus_tag="VGM72_12130"
     CDS             14707..15180
                     /locus_tag="VGM72_12130"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HEY4266059.1"
                     /translation="MTWLEGSALGHAMRDSGVWTYGIVNLTHILGVATLFGSIIILDL
                     RLLGLWKHIPLSALSRPTVAMAAIGLGVAVLSGSGLLATKATEYVGNPFLYIKFPAIA
                     LGLFNVWLLHRSAAWRAHRVRALSPREESQLRAMGGTSLLCWLTAISAGRMIGYW"
     gene            complement(15193..15483)
                     /locus_tag="VGM72_12135"
     CDS             complement(15193..15483)
                     /locus_tag="VGM72_12135"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY4266060.1"
                     /translation="MIIESAILSIKPGQGPAFEAALKNARPLIEATRGFVGLEVRSCI
                     EHANRYLLLVRWENLEDHMVGFRQSDRFQKWREALHHFYDPMPDILHFRDPV"
     gene            complement(15488..15907)
                     /locus_tag="VGM72_12140"
     CDS             complement(15488..15907)
                     /locus_tag="VGM72_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004278705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEY4266061.1"
                     /translation="MAYDSNNIFAKILRGEIPAVKIYEDGKTLAFMDAMPQADGHALV
                     IPKEAAENIFDLSSSGAAALMATTQKIAKAVKKALNTPGIMIVQLNGASAGQSVFHVH
                     FHVIPRQGGADFKLHAREMETPEKLGLLADRIKAALE"
     gene            complement(15996..17159)
                     /locus_tag="VGM72_12145"
     CDS             complement(15996..17159)
                     /locus_tag="VGM72_12145"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4266062.1"
                     /translation="MTKIRPGLGASAHEIGADTWDFLANPERRPLPHPFTRHAFFAAL
                     EDSGSATAKTGWRPMHLLLERDETPFALLPLYLKSHSYGEYVFDHGWADAFERAGGRY
                     YPKLQASVPFTPATGRRLLIAAGENEIQAGQILLDAAESAVDQLRASSLHITFMTERE
                     WDLAGTRGFLRRTDKQFHWENRGYDSFGAFLDDLSSAKRKNMRKERASILAEGVSFEW
                     LTGSDLTETVWDEFFSCYTTTGNQKWNPPYLTREFFSRVGMSMGEQILLVMAKRDGRY
                     IAGALNFFDGVTLYGRNWGAIGYVPLLHFETCYYQAIDFAISKGLARVEAGAQGGHKL
                     LRGYLPKPTYSAHYIAHPGLRRAVADYLANEREAVAEHIEELMEQAPYKKTTL"
     gene            complement(17156..17623)
                     /locus_tag="VGM72_12150"
     CDS             complement(17156..17623)
                     /locus_tag="VGM72_12150"
                     /inference="COORDINATES: protein motif:HMM:NF025939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY4266063.1"
                     /translation="MTGRIAAKLIESGITLPVPAAPQGAYVPWVKSGALLFISGQITM
                     GARGLEYVGTVGREINLEDAKAAARLAAINVIAQAAAALDGALDRVSRVVKVTGFVNA
                     VPGFTQHPEVVNGASELFADIFGDAGKHARAAVGVSSLPRNVAVEIEAIFEIA"
     gene            17729..18778
                     /locus_tag="VGM72_12155"
     CDS             17729..18778
                     /locus_tag="VGM72_12155"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEY4266064.1"
                     /translation="MNRTLDAASPSPLEDGYVFPAEWHPHRRTWMCWPVRTACFGGAD
                     GVLRAKQAYARVARAISAFEPVRLAARTQEVAEARFATGGKVEVVEMALDDSWARDFG
                     PTFLKNDSGRLAGVQWIFNAWGRKYHPYTNDETFARRVLEMIGADVYAAPLVCEGGAL
                     HADGEGTVLTTEQCLLHPNRNPGRTREEVEDVLKSHLGARTVVWLGSGFSDTETDGHI
                     DNIACFAAPGRVILGMPAARSHPDYAPVQEAMSRLKAARDAEGRAFEIVPLPQPRKHR
                     QSWSGRPLAMSYVNFYLANGAVIMPAFGDPNDEDARTILAQCFPGRDIMQIDALDIVQ
                     GGGGIHCITQQEPAA"
     gene            18775..19647
                     /gene="aguB"
                     /locus_tag="VGM72_12160"
     CDS             18775..19647
                     /gene="aguB"
                     /locus_tag="VGM72_12160"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR03381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEY4266065.1"
                     /translation="MSTVTLAAVQFACTWDLAGNIAKAKGMVRRAAAAGAQIVLLPEL
                     FETPYFCQDQSAKHFALARSFEGNPIIAEFARLAAELSVVLPLSFFERAHNAHYNALA
                     MIDADGRVLGRYRKSHIPDGPGYQEKFYFRPGDTGFQVWKTRHGELGAGICWDQWFPE
                     SARIMALKGAEILFYPTAIGSEPAPAPPVDSQAHWRRVMQGHAAANCVPLVASNRVGA
                     ERGEAGEIIFYGSSFIAGPTGEILAEMGREEEGIIVADFDLAAIAKARASWGLFRDRR
                     PDLYGPILTADGET"
     gene            19764..20876
                     /locus_tag="VGM72_12165"
     CDS             19764..20876
                     /locus_tag="VGM72_12165"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HEY4266066.1"
                     /translation="MHAGRTAIVLLAGAALLAGAATSACAQQNPAPKPTDLPNPYRLV
                     EGWPTLPKSMNGGHWGEVIRVHVERDGNIWVFHRCFNTVPPGSAVCINRGPANPPILE
                     FDPSGKLLKSFGAGLFAYPHGFTVDQDGNLWVTDVNDAETVLGMSARNAAGVVMGQEV
                     LKLSPTGKVLMTLGKEGVSGNGIDTFDRPTGVAIAPNGDIFVTDGHLPNAHNNGRVLK
                     FSKDGKFIKQWGRKGSAPGEFDEPHDIFLGGSQNRIYIADRRNQRIQVFDQEGNFIAA
                     WKQFGEPSSVFVGRDDTIYVGSGFPDPTAKRGEIRGIVVGNAKDGSLKAFIPDPADPN
                     SLSRGTTSSGIAADDEGSIYSADVAAHNLRKYVKVK"
     gene            complement(20983..21255)
                     /locus_tag="VGM72_12170"
     CDS             complement(20983..21255)
                     /locus_tag="VGM72_12170"
                     /inference="COORDINATES: protein motif:HMM:NF023518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3572 family protein"
                     /protein_id="HEY4266067.1"
                     /translation="MQRSAAETLALKALAYLAADGDGLIRFLTLTGLEFDDLRARAGN
                     PELLAGVIDFLLSDEALCTGFLAAEDLDSPMLHAARRALPGVAPDG"
     gene            21394..21768
                     /locus_tag="VGM72_12175"
     CDS             21394..21768
                     /locus_tag="VGM72_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4266068.1"
                     /translation="MDASAKTVLIVEDNELNMKLFHDLLDAHGYRILQTKDGMEALDI
                     AREHRPDLILMDIQLPEVSGLEVTKWLKEDDSLRAIPVVAVTAFAMKGDEEVIRQGGC
                     EAYISKPISVTSFLETVRRFLG"
     gene            21786..23150
                     /locus_tag="VGM72_12180"
     CDS             21786..23150
                     /locus_tag="VGM72_12180"
                     /inference="COORDINATES: protein motif:HMM:NF007135.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PleD family two-component system response
                     regulator"
                     /protein_id="HEY4266069.1"
                     /translation="MTARVLVVDDILSNVKLLEAKLAAEYFEVITASNGEQALTRVAA
                     ELPDIVLLDVMMPGMDGFEVCRRIKANSKTTHIPIIMVTALDQPSDRVTGLHAGADDF
                     LTKPVDDTALFARVRSLVRLKMMTDELRMREETGQSMGLADAGETLFEADPTGHILIV
                     EDRPESVAWFRAVLETKNKVADARNFDEALLLSRGGDFDLIVVSLGIRAFDGLRLCSH
                     LRSLAEIRNTPILVLVSDGENRKLAQALDMGVNDYLMRPVDRNELMARVKTQLRRKRY
                     ADKLRHNMQLSLEMAITDQLTGLNNRRYMARHLETLLKNASETKPISFLIMDIDYFKT
                     VNDTHGHDVGDEVLREFAARISANVRGIDLACRYGGEEFVVVMPDTDKEFAHSVAERL
                     RHTMELMPIPISRAPNKIVITVSIGIASSIGGDDNSDKLLHRADQALYRAKREGRNRI
                     AEAA"
     gene            complement(23199..23687)
                     /locus_tag="VGM72_12185"
     CDS             complement(23199..23687)
                     /locus_tag="VGM72_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266070.1"
                     /translation="MDSFRHKIALIVVLAIALSGCNRNSQQNPAPPAPAPAAPAAPLP
                     APPPPPPPIPASTELPLKSVDSVMLSRPPDGPMALVIQVSGTAPSPGWTDAKLAEEPD
                     NGGDASIKTYRLVATSPAMPDENRTVQTIEAELRVDLLAPEVKTIRIVSATNEISAPV
                     AQ"
     gene            complement(24666..25451)
                     /gene="xth"
                     /locus_tag="VGM72_12190"
     CDS             complement(24666..25451)
                     /gene="xth"
                     /locus_tag="VGM72_12190"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY4266071.1"
                     /translation="MKIATWNVNSVKARLDAVTTWLAEAAPDIVCLQEIKTADGTFPS
                     EIFEALGYNCTVHGQKSYNGVAILSKRPPEDLTRGLPAVEGDDHARYLEAVVPGESGG
                     IVRVASIYAPNGNPPGTERFAYKLAWLERLNRHIRHLLTFEEPLILTGDYNVIPAPDD
                     CNDPKAWAGDALFQPETRAAFRRILHLGLTDAFRACHAEPRCYTFWDYQAGAWVKDHG
                     IRIDHILLSPQAADRLVSCSIERKVRGWEKPSDHVPVLCELAI"
     gene            complement(25462..25809)
                     /gene="erpA"
                     /locus_tag="VGM72_12195"
     CDS             complement(25462..25809)
                     /gene="erpA"
                     /locus_tag="VGM72_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020056313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEY4266072.1"
                     /translation="MSVETIQSPPVRLTDRAAKRIAEILREETGASNLRVAVSGGGCS
                     GFQYDFCFDDSRAEDDLLIAQDGARVLIDSVSLDFLKGAEIDFVEEMIGAAFKINNPN
                     ATSSCGCGTSFSI"
     gene            25856..27073
                     /locus_tag="VGM72_12200"
     CDS             25856..27073
                     /locus_tag="VGM72_12200"
                     /inference="COORDINATES: protein motif:HMM:NF002328.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEY4266073.1"
                     /translation="MSKTPPSGRLLGPLFVPPPRAPYATNPASSRGRFWAEEESATRS
                     CYQRDRDRIIHSTAFRRLKHKTQVFVQHEGDYYRTRLTHSLEVAQIARSLARVLGLDE
                     DLAEALALAHDLGHPPFGHAGEAALNACMAGHDGFDHNAQALVLVTRLEHRYADFDGL
                     NLTWETLEGLVKHNGPLLHEGAGLRESLPEAIAAFPWDLELFTHAGPEAQIAALSDDI
                     AYVNHDIDDGLRAGLFQIEELFEVALVGPIFRELSAAHPGLERGRLIGEAIRRLIGQM
                     VGDVTAQTLRALGALAPRNAGEIRGYGTPLAEFSPALRADLSKLKEFLFRRMYHHPRV
                     LQVMQNAQLALTQLFGTFMADPALLPRDWMAGCGAPGDSVTARAVCDYIAGMTDRFAL
                     QEHRKFFHMEIAL"
     gene            27158..>27930
                     /locus_tag="VGM72_12205"
     CDS             27158..>27930
                     /locus_tag="VGM72_12205"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY4266074.1"
                     /translation="MNTHQRGVYQPAMDNAQVYDLSNEEVEEEEHSRLPLLIVIALLV
                     LAAFAGVVWLAYNQGVARGRAGAVVVIAAPDGPVRTAPLDPGGTPAPYTGLKVYGQSL
                     PPDQEAQGTTLAPQAPAGIPASSPAQRPQPAPAVVTSDTPPVRLNPDAPTLTASKPPP
                     PAAVKPAVQTAAATSAAAVAAAVPSQSAALTSAASGKAVLQIGAYESPEIANGAWTSF
                     KSRYADVAAKLGEDVQKADLGAKGTWYRLRVGPFADKAAA"
BASE COUNT         5510 a         8393 c         8643 g         5384 t
ORIGIN      
        1 caatccttcg acgtctacac caaccaggag aatttcttca cggtaaattt tccgggcacc
       61 cctacggcaa cgcaggtgac gcataagacg ctgaaaggca cggaattgac cgcgcatgtg
      121 ttcaccgcga cggtcccgcc cggggccagg acgagcggaa cctactcggt caccgtcgtg
      181 gactttgcgg gcgcactcga tgagatgagc acggccgtgg agcacgcggc cgacgccatt
      241 cgcgcaaagg gcgccataaa atacgatgcc atcgagaatc tcgatcttca tgcgtcccgc
      301 cgcctgacgg tcgaaaccgc caccgaccga atcctggcgg aaatcctttt tgcccccaac
      361 aatcgccttt acatcgtgca agcggaaaat tctctgaccc agacgccacc cgcgcaattc
      421 caagcctctt tgcaaatcat cgacaacgcc ggcgcgcgca tccggacaag aacgctgtta
      481 gggctgccgg aaggatcgaa gcagcccttg aatgcgggcg gcattgcgga cgagccggac
      541 acggtcgcgg cgctgatgtc gggctcatgg cgcagcgcgg gcggcacctg cgaggcgccc
      601 tatttcaaat cgggcatgcg ctccaagaac agccggggcg aggcggttat ggccggcacg
      661 atcaccaatg gcggaaccac gatcaacggg atgctcatcg tcgaagccgc gcgcgcgggc
      721 caattcgtcg atccggtttc gtttcaagcc ttgatgctgt tcgatccgat gggcgacaag
      781 ctcggcattt cctcaatcgg cggggccgcc gcgagctggc cggatgtgac actcgagctt
      841 tgtccgggaa gccgcacgta agaaacgaga cgcggccggc tttgccggtg aacgtgtccg
      901 attttcaggg gaaggggata cccggcctgc gcccctggag atattgtctt ggaacgcagg
      961 attttcgagg tgggggagtg tcatgaggaa ttggtttgcc gcatcgctgc tgatcttggc
     1021 gcctttgatg gccgccgccg ccgaggcgcc tccggaatgg gcctatcccg ccgcccccgc
     1081 cggtttccag cctccaccgg atgacggcca gcccaaacac gtcgcgggca gcacgaagac
     1141 cttcaccgag aaggacgtca acaacatcca caatccgccg gactggtttc ccggcgaaca
     1201 tcccccgctg cccgatctcg tcgcccacgg caaggagggc gtgaacgcct gcgcgcaatg
     1261 ccatctcgcg accggctacg gccatcccga atcggcgaat ctttccggtc ttccggcgga
     1321 ctatgtcgag gagcaattga acgccttcaa gagcggcgac cggcgcagct cgctgccggg
     1381 ccgttccgcc aacatgatca atttcgccaa ggcgatgaac gaatccgaca tgaaacaggc
     1441 cgccgcctat ttcagttcga taaagccggt ggtgtggacc aaagtgaagg aagccgcgac
     1501 cgtccccaaa acctatgtgg gtgagggcaa tatgcgtttt gtctccaccg gaacgcagac
     1561 cgaggccatc ggcaaacgca tcatcgaagt gcccgaagag gaagaaggcg cgaaggtccg
     1621 cgatcctcat tcgccctttg tcgcctatgt gccggtgggc agcatcgagg ccggcgaaac
     1681 gcttgccact acgggcggcg gcggcaagac gatccaatgc agcgtctgcc atggcgcgga
     1741 tttcaagggc atcggcaatg tgccgcggct cgccgggcgt tcggcgattt atattttccg
     1801 ccagctcaac gatatccaga agggcacccg gcacggcaac gcggtggcac tgatgcagcc
     1861 ggtggttgcc aagctttccg aggacgacat gatcgcgctg gctgccttca tggcctcgcg
     1921 gaccccgtaa atagcggcag aaaacccgtt cttggcgaat gggaacaatt gatgttgggg
     1981 gggccttccg gctatcttcg cgccgagcgc ataaaacgga gggatcgatg agaaaactgc
     2041 tgctgatatc ggcgattgcc atgttgcccg cgggggcctt cggggctgaa tcggcgttga
     2101 gttgggccta cccggtcgca ccgcaagggt tgccgcgtcc cgatcccaac aagaccttcc
     2161 aggccaaggg cgccccggcg gatatgaagc tgaccgcggc gcagatcaat aacgccttcg
     2221 gaccgccgga ctggttcccg aacgaacatc ccgtcatgcc gtccatcgtg aagaacggcc
     2281 gcgagccgca tgtaagggcc tgcatgctct gccatcttcc caacggcaac gggcatccgg
     2341 aatcggcgag cgtttcgggc ctaacggcca cctatatcac cgagcagatg cacgaattcc
     2401 gcgccggcaa ccgcatgaac aatcgtgcgc ccgtcatgat cgagatggcc aaggacatca
     2461 cggagccgga gctgaaggag gcggccgagt atttcgccag cattccgaag gcggagcaga
     2521 aatggatccg cgtcgtcgaa ggcaagaccg cgcccaagaa ccatgtcggc aacggcggta
     2581 tgcgcttctt cgatgaaggc ggcggcaccg tcgcggtcgc gcccaacatg atctacgaag
     2641 tcgccgaaga cggcgaagcc gcggagcttc gcgatcagca ttacggtttc gtggattatg
     2701 tgccggaagg cagcatcaag aagggcgaaa ttctcgccac cactggcggc ggcggcaaga
     2761 ccattcagtg tgccgtctgt catggcgccg acttcaaggg catcggcgat gtgccgcggc
     2821 tcgcggggcg tggcgcctat tatctcatcc gccagctcaa cgacatcaag atgggcaccc
     2881 gcaaaggcaa cgcggtcgcg ctgatgaagc ccgtggtcga gaaattgtcg gacgaggata
     2941 tggtcgatct cgtcgccttc atggcgtcgc gccagccgta aggatgtttc acacggccgc
     3001 tgtgtccgtg agaggatgcg gcggccgttt tgttcgggcg gtagaattct catcgtgaca
     3061 gtgcatctca tcaagctctg cgttggcgcc gacagcatcg aagacctggc cgactggcag
     3121 aagaaacgta gcgccgaacg gacgaagaag aacgaaaacg gcgacatcct gcacatcacc
     3181 cgcatgacgc ccaagcgtgg cgatgagttg ctgaagggcg gttcgctcta ttgggtcatc
     3241 aagggccaga tcgcggtgcg tcagaagctt ctggcgctga agccggtcgt gcgcgagggc
     3301 gtgccccatt gcgcgctggt gctggacaga tcgctcgtcc cgacggtgcg gcgaacccat
     3361 cgcgcgttcc aggggtggcg ctatttcgat cccaaagacg ccccgcgcga tcttaaagct
     3421 agcgataaat ccatcccgga aggtttgcgc gcggaattga cccaattggg tttgctttag
     3481 accgccatgt tgtcgatcag ccgcacattg ccgatacgcg ccgccgccag cacccgcgcc
     3541 gggcccgata tcgcacgcag gggcgcgaga gtttccgcat cgcgcagggc gacgtagtca
     3601 acgcggtcga agcccgcatt gagcagggcg gcaccgcctt ccgcctcggc ctgcggaatc
     3661 gaaacaccgg atttcgcgcg ctcggccact gctcgcagta cctggttcag atgcgcggcg
     3721 gcgctgcgcc gctccgcgtt cagatagaca ttgcgcgagg agagcgcgag cccgtcgcgt
     3781 tcgcgcaagg tcgccacgcc gacgatgccg agcggcaggt cgaggtcctt taccagccgt
     3841 ttcaccacga gcaattgctg atagtccttc tcgccgaaga tcgcgatgtc gggcgcgcac
     3901 tgcacgaaca gcttcgtgac gatcatcgcc acgccgccga agaaatgcgg ccggaaatcg
     3961 ctttccagcc ccgcggccgg gccgtccatg acgatacgcg tggcaaaacc gtccggatac
     4021 atgtcttttg cgttgggggc atagatgaga tcggcgtcac cggccgtctc aagaaactga
     4081 cggtccttgg tcatgtcgcg cgggtagcgt gcgaaatcct catggggcgc gaattgcgcc
     4141 ggattcacga agatgctgac cacgatcttg tccgctcgcg ctttggcttc gcgaacgagc
     4201 gcgagatgac cgtcgtgcag tgcgcccatc gtcggcacca gggcgatgcg tgcgcctttg
     4261 ttccgccaga gccggatgcg caatcgcagc tcgaccgcat cggatgcggt ttctatgcgt
     4321 ttggaagagt tcatcgcatg agattaccgc aggcgctcct ctctgcgcta tcctggatac
     4381 aaattcagct tgccggccct atcggacaaa tccatggaga accttcgtca gacgatttct
     4441 gcggcctatc ttgccgatga ggatgcggtt ctcgaaagcc tgatcgcgaa ggccaagatg
     4501 accccggccg aagccggcac agccgaggcg ctcgcgcgca atctcgtttc gcaactgcgc
     4561 gggggccgcc tgcgccatag cggtgtggac gcgttcacgc gggaatatgc gctttccagt
     4621 gaggagggcg tgatgctgat gtgcctcgcc gaggcgcttc tgcgcgtacc ggacgcggag
     4681 actcaggacc ggctgatccg cgacaagatc gctggacact cctgggaccg ccatcttggc
     4741 cactctgagt cattgttcgt caatgcttca acctgggccc tgatgttctc aggccgcctg
     4801 gtggaaacgg gggacgcctc gcgctgggat ttcgaggcga tctggcaccg tatcgccgca
     4861 cggctggggg aaccggtgat ccgtcaggcc gtgctgagcg cggtacggtt tctcggccgt
     4921 catttcgtgc tcgggcgcaa catggaagaa gcgctgaaag aggcacgtcc ctatgccgag
     4981 cgcggccacc gattttcctt cgacatgctg ggcgaggccg ccgttacgcg cgccggggcc
     5041 gggcgctacc ttgaaaaata tcgcgaggcg gtccgcgcca ttgcacgcgc ttggccgcag
     5101 cgttcggttg catcgatcga acgcccgggc atctcgataa agctgacggc gctccatccg
     5161 cggttcgaat acgccaaacg ccatgaggtt atgttggaat tgcttccacc gctcacccgc
     5221 ctgtgtgcgg aagcgcgcga agccaatctt ccggtcacga tcgatgccga ggaatccgaa
     5281 cgtctcgact tgacgctcga tattttcgaa gccttgggcg atgaagaaag tttgcgaaat
     5341 tgggacggtc ttggcttggc ggtgcaggcg tatcagaagc gggcgcttcc ggtgattgca
     5401 tggctcatcg atcttgcgcg ccatcggcgg cgtatcattc ccgtgcgcct ggtgaaaggc
     5461 gcctactggg acagcgagat caaacgcggc caggaacttg gcatttcaga ctatcccgtc
     5521 ttcacgcgaa agatcggcac ggacacgtcc tatcttgctt gtgcgcgcgc ccttctgtcg
     5581 gcgccaaaca atatctttcc ccaattcgcc acgcataatg cccatacgct ggcggcggta
     5641 caggcgttcg cgggatcgag tcgaaaattc gaattccagc ggctgcatgg catgggcgaa
     5701 acgctttacg aaatctatga agagatcatg cgtcccgcgc atgcgagcgc gacacgcatc
     5761 tacgcaccgg ttggaaacca cgaggatctg ctcgcctatt tggtgcgccg cctgcttgag
     5821 aacggcgcca atacgtcctt cgtgaatcgc ctggccaatg acaaggctcc tctggaagac
     5881 atgatcgccg acccggtgga gcgcttgatc gccaccgcgc ccaaacgcaa tccgcgcatc
     5941 gcgaagcccg ccgatatcta tcccgggcgg atgaattccc acggctttgt gttgagcgat
     6001 ccagcggaga gcagccggat catctccggc atgcgccgcg cgatcgatat gggaccacac
     6061 actgcgaatc caattctcga tggtgtggaa cacatccggt ccgcaaagcc ggttcgcgat
     6121 ccggccgatc gcggccgcga aatagggctg gtcagcgagg ctgccgatga agatgttcgc
     6181 acggcgctcg agagcgcggc gacggcgcag gactcctggg accgccaggg aggtgccttc
     6241 cgcgccgcca tcctggaacg tgccgccggc cttttcgagg accgccgtgc gctgttgatg
     6301 gcgctgattg tgcgcgaagc gggccgcacg gttgcgaatg cgctgagcga atggcgcgag
     6361 gccgtcgatt ttctgcgtta ctatgcgaag gaagcccgga ggcatttcga aggcgcgctt
     6421 ccccttcccg gcgtgaccgg tgaggataat cggcttgcgt tgcgcgggcg cggggtgttc
     6481 gcctgcatcg cgccgtggaa ctttcccctc tctattttca cgggtcagat cgccggtgcg
     6541 ctggcatcgg gcaacagcgt gctcgcaaaa ccggcggagc aatcgcctct cataggtgcg
     6601 gccgcggtac gcttgctgca cgaggccggc gtgccgggcg aggttctgca tttcctgccc
     6661 ggagacggcg cgcgcatcgc aaagacgatg ctcgccgatc cacgattggc aggcgtcgcc
     6721 ttcaccggct cgaccgaaac cgcgatcgcc atcaaccgcg cccttgccgc gcgcgatggg
     6781 ccgatcgccg ccttgatcgc cgagacggga ggcctcaatg cgatgatcgt cgattccacg
     6841 gctttgcccg aacaggtcgc acgcgacgta ttgatttccg ccttcgacag tgccggacag
     6901 aggtgttcgt ccttgcgcgt gctgttcctc caggaagacg tcgcggatcg catgctcgag
     6961 cagatcttgg gtgccatgga aatgctgaga cttggcgatc cattcgacct tgccaccgat
     7021 atcgggccgg tgatcgatga agaggcccaa gccgcgctcg cggcccatgc cgaccgcatg
     7081 acaaaggacg caacgctctt gaaaaagctg accgtcgatc ccgcgctttc gagaggtgtc
     7141 ttcttcgccc cgcatgtgtt cgccatcgaa tccatgaatg cgctgaagcg cgaggtgttt
     7201 ggtcccattc tccacgttgt gcggttttcc ggcgatcggc tggacggtgt atgtgacgcc
     7261 atcaatgcga gcctctatgg gctgacgctc ggcatccaca gccgcattga caagacggcg
     7321 gaattcgtgc gccagcggac gcgggtcggg aatatctacg tcaaccgcaa ccagatcggc
     7381 gcggccgtcg gcgcccaacc ttttggcggc gaacggttgt ccggtaccgg accgaaggcc
     7441 gggggaccgc attatctgcc gcgttttgcg gtagagcgca cgctcacgat caattccgcg
     7501 gctgccgggg gcaatgccag ccttctaacc ttggacgaag catgagcgcc gttttctcct
     7561 aaaatgcgag gatacggggg cgattcgagt gccggatcag gtggacgggc gaagggatga
     7621 aaccatgatg atgtcgacgg ctggcgacag gccggccatt gctgacgctc ccgctaaacg
     7681 cgtccatgtg atcgtcttcg gcaacgagaa aggcggcacc ggcaagacta ccaccgcgat
     7741 gcatgtcgcg gtggcgctgt tgcgtatggg aatgaatgtg tccgcgatcg atctggacag
     7801 ccgccagcgc actttcgcgc gttatatcga aaaccgccgc gatttttccg ctcggaaggg
     7861 gcacacgctt cgttcgccgg acgtgcaggt ggtggagcgc gccgtctctt ccgccgatgc
     7921 cgttgcggac gaaagtacgc ggctcgccga agcactcacc cgggccaagg agggcgcgga
     7981 tttcgtcatc atcgatacgc cgggcaatga cacgttgttg tcgcggctgg gtcatgccgc
     8041 ggcggatacg cttgttacgc cgatgaacga cagttttctg gatttcgatc ttttggcaaa
     8101 gctcgacccc gatagcctcg aaatcaaaag cccaagtctt tatgcggaat tcgtctggga
     8161 ttgccgcaag cgccgcctga tcgcgagccg cgccaacctc gattgggtgg tcatgcgcaa
     8221 ccgtgtttcc gccacggagg cccgcaataa acgcaaggtc gcgacagcgg tcgaaagggt
     8281 gtcctcccgc atcggttgcc gcatagcgcc gggtttttca gagcgtgtga ttttccgtga
     8341 gctgtttccg ttagggctca ccatgctcga tctgccgctg ccagggcttg ccatgccgct
     8401 caccatgtcc catatcgcgg cgcggcagga agtgcgcgag ctcctctcga cgctgcgcct
     8461 gccgggagtg gaaaaggcgt ccggcacgaa ttagccggcg gaagactcta tgccagcttt
     8521 ggtcgccggc gttgtgttgc ttggcctgct gctgttgatg gcgcggggct atacaagcgc
     8581 gaacgcgaga agtctcgcca agggcctgcg cttctcgggc ggcattatcc tggcgctgtt
     8641 gacggtagcc ttggcgatca cgggccgcgt tgcgttcgcg ttccttgccg cgggcggtgc
     8701 gtggtatctg ctttttggtt cacctccgcc ctggcaaaga acggcttacg gtgccggcgg
     8761 tcctggtggc agccagaatc gttcgcgatc gtctccgccg caaacaggcg cgatgtcgcg
     8821 cgtggaagcc ctgaaggttt tggggcttga agagggggca agcgaacagg acatccgagc
     8881 ggctcaccgc cgtctgatcc tccaaacgca tcccgacaag gggggaacga gttatctcgc
     8941 ggccaagatc aatgaagcca aggacgtgtt gctgcggcgg cgataaaact taaccggcgt
     9001 cggcggcggc tacgaagcag ctttgtccgc gttgttccat gcgtttgcaa atgtcgcgtg
     9061 cttggccttc ggcgaacatg cccaaacggg cgcggtacat gatacggccg ccggatgtga
     9121 aggggatcac cagtttctgc gcctgaccga tgacgtcgaa tccacttctt gcaaaaaccg
     9181 cgagctttgc ccgggccaat gtctcactcg cgaaagcgcc gatctgaacc gcccagcgct
     9241 tcaccgtggc attgctggcc agcgtggaac tggacgacgt caaattcgcc aaaaccggag
     9301 cagccggcat tggaacagcg tggagtgtct ttgccgggac tggattggcc gcccacgcaa
     9361 tcactggcgc cggattgggt agctcagtcg catcggcatc ggttccgccg tcaccttggg
     9421 caagcggaac cgaagcatcg gctatctgct gcgcggaagc cgggtcgaac tggagcggtg
     9481 tccgcacctt tcccagcgga gcttcgatga cgtccgtctc acgagcgaga accgtctcgt
     9541 gagaaagaac tggggcagcg gtctccggat aagtctcact ggcaggcttc gaaatcggca
     9601 ccacggtcaa acccgatccc gcgccaggga ccttcttggc gtccgccacg agcacgggag
     9661 gaattttcgc tgtggacttc gccttcgact tgggctcaag ggcagccagc agcaatggat
     9721 tgtcttcccc ctggatggct ttgaagaggt caggtccagg cattttgccg cccttccagg
     9781 gcacatcgat ctcggcaagc atatccgggt ccgacttggc ggcttcgaat gcggccgaca
     9841 gcaaccgcat catttcacga tcgcggacgg ctgctgttgg ccctcccaga acaacgccga
     9901 tcacatgttt gtcgttacgg accaccgacg tcacgaggtt gaaccccgag agctgcgtat
     9961 agccggtctt gatgccgtcc gtacccccga atgccgcaag gagattgtca tgcgtggcgt
    10021 aggagcggtt gccatagctg aagccgggcg ccgaaaaata atggtaatat tgcgggaaat
    10081 cgtaagccag gtgacgagcg agaagcgcca tgtcgcgcgc ggtggtgagc tgctgcagat
    10141 cgggcagtcc cgacgcattg tggaaattcg tgtgcgccat gccaagctcg tgggccttct
    10201 gcgtcatcat gaccccgaag gcgtcttcgg tgccgccaag cgcttcggcg atcacgacgg
    10261 cgacatcgtt cgcggacaat acggtcagag ccttgatggc ggtttccacc ggaatcgcgg
    10321 cgcccggtcc catggcgagc ttggtgggtt cttgttcgga ggcatggacg gacgtgataa
    10381 gcggagtgtc gagacggacc ttaccctggt cgagggcctc gaaaagcaaa tacagcgtca
    10441 tcatcttggt gagggacgca gggtagcgaa tcgcatccgg attgcgcgca tagagcagtc
    10501 ggccggtcgc accgtcgatc acgatggctg catctttttc cggggccggc acgatcgggc
    10561 ggctgacgcg tatcgatctc ttcccggccg cggcggccgt ggatgcggtt ccaaataaag
    10621 gaaggatgac caatagagcc gccaggcaag caaaaagaaa tttgggcctg atccggctgc
    10681 tatcgtgcat taatgtcccc tttagactga aatgcgccgt ggcgtcacga aatgagacca
    10741 aattatgttg agtctcgcaa gggtctcagg accgaaagcc tagttcttgg tttcccaagg
    10801 tctggttaac gcctatctca cgaatcagta tacccgcatt ggttcctggg agaaccgcga
    10861 aaattttcac tttccggtta gatttggtgc ggtgcaacat tttttcttga caatcttctg
    10921 ccacatacgc atatagcaaa tgctgcattg cagcaatttg gcaaaagcga ggaacagatg
    10981 gctaccaaga aatcgagcac cccgggcact gagcccatcg aaaccgcgct tctcaccggc
    11041 gccgaagcca tgaaagacgg cttcgagaag gctgtgaagg gttacgatca aattgtttcc
    11101 ttcggcaagg acaatgccga agctgttctc aaatcggcca atgcggccgg caagggcatc
    11161 gagacgatca acagcgaagt tttcgcctat gtccgtaaat ccgccgaaga cagtgtcacc
    11221 gcggccaaag cggtcctttc ctccaagacg gtggaagaag cctttcagct ccagagcgag
    11281 ttcggcaagg cgatgttcga aacgtatgtc gatcagctgg cgaaattcgg cgatatggcc
    11341 ctggccaccg cccgccacac ggcggagcca ttgcaagcac gcgtttcagc cttctcggaa
    11401 ctggtgcgcg ccgcgtaatc ggcaaacatt ccagtgatta agcgcaggcc tcggacgatt
    11461 gttcggggcc tttcgctttt tttacagccc tcgtaacctt atcttcgttg ccagcgtttt
    11521 atttatgcca ggcttggaag cgtatgaacg aaggccattt cgacaaaatc ttcagaccca
    11581 ggaatgcggg tcccacgcgc gggcctgggc aaggcgatgg cgattcggga gatcgcggca
    11641 tcggcaccgg cgtcgtcacc aagacgcgtg ccaagacgaa aaaaccatcg ctttacaagg
    11701 tcctgatcct caacgacgat tacacgccga tggaattcgt cgtgcatgtg ctggagcgct
    11761 tcttcgccaa gcagcgtgag gaagcggtgc agatcatgct ccacgttcat cgacaggggg
    11821 tcggcatctg cggcgtttat acgtttgaag tcgccgagac taaggtggcg caggtgatcg
    11881 agttggcgcg acgccaccaa catccgctac aatgtacaat ggagaaagag tagttaacgg
    11941 ctatgccctc attatcccgc agtctggaac aggcgttgca ccgggcgatc aggctcgcca
    12001 gcgaacgcca tcatgaatat gcgacgctgg aacatctcct gctcgcgttg gtcgacgatt
    12061 cggatgccgc ccaggtcatg cgcgcctgca atgtcgatct cgacgcgctt cgggccgggc
    12121 tcgccaaata tgtcgacgag gatctcgcga cgctcacgat cgaagacggt gaggacgcca
    12181 aacccacgac cggcttccag cgcgtggtac agcgtgccgt cctgcatgtg cagaattccg
    12241 gccgcgacga ggtgacggga gccaacgtgc tggtggcgct gttttctgaa cgcgagagcc
    12301 atgcggtcta cttcctgcaa gagcagaaca tgtcgcggct cgatgccgtg aactatatga
    12361 gccatggcat cgccaagcgc cctggcatga gccagcccaa ggccgcgcgc ggaacggatg
    12421 aggaagaaga aagcggcgag aaaaccgcga agcagggcac cgatgctctg gaagcctatt
    12481 gcgtcaatct caacgacaag gcgcgcaagg gccgcatcga tcccctgatc gggcgtgagc
    12541 ccgaggtcga acgcactatc cagatcctct gccgccgcag caagaacaac ccgcttttcg
    12601 tcggcgatcc gggcgtgggc aagactgcga tcgccgaagg tctcgcgcgc aagatcgtca
    12661 agggcgaagt gccggacgtg ctgaaggcct gcaccatcta ttcgctcgac atgggggcct
    12721 tgatcgcggg tacgcgctat cgcggcgatt tcgaagagcg cgtcaaatcg gtcgtgaagg
    12781 aattggaagc gctgaagggc gcgattcttt tcatcgacga gatccacacc gttatcggcg
    12841 ccggcgccac ctcgggcggc gcgatggacg cttcgaatct tttgaagccc gccctgcagg
    12901 ccggaacgct gcgctgcatg ggatcgacga cttacaagga atatcggcaa tatttcgaga
    12961 aggaccgcgc gctcgtccgc cgttttcaga agatcgatgt ggccgagccg accgttccgg
    13021 acgcgatcaa gatcctcaag gggctcaagc cctatttcga ggattatcac aaggtccggt
    13081 acaccaatga cgcgatcaag gcggccgtcg aactcgcggc gaaatatatc aacgaccgca
    13141 agctgcccga taaggccatc gacgtcatcg acgaggtcgg cgcttcgcag atgttgttgg
    13201 ccgagaacaa gcgcaaaaaa gtgatcggcg tgcgcgaggt cgaggacgtg gtggccaaga
    13261 tcgcgcgcat tccgcccaaa tcggtttcca aaacagatgc cgaagcgctg cgaagtcttg
    13321 aagacgatct caaacgcgtc gtctatggcc aggattcggc catccacgcg ctgacgtccg
    13381 cgatcaagct tgcgcgcgcc gggctgcgcc aggcggaaaa accgatcggc tgttatctct
    13441 tctcggggcc cacgggtgtg ggcaagaccg aggtcgccaa gcagctcgcc cgcgtgatgg
    13501 gcgtcgaact cctgcgcttc gacatgagcg aatatatgga gcgccacacc gtatcgcgtc
    13561 tgatcggcgc gcccccgggc tatgtcggct tcgaccaggg tggattgttg accgacggca
    13621 ttgatcagca cccgcattgc gtgctgctgc tggacgagat cgaaaaggcc catatggacc
    13681 ttttcaacat ccttctgcag gtgatggatc acggcaagct gacggaccac aacggcaaga
    13741 agatcgactt ccgtaatgtg gtgctgatca tgaccaccaa tgcgggggca tccgacgcgg
    13801 ccagggaatc catcggcttc ggacgcggaa aacgcgaagg cgaggatgag gaagccatca
    13861 agcgcttgtt cacgccggag ttccgcaatc gtctcgatgc cgtgatcgcg tttgcgcccc
    13921 tcacgcgcgc caccatcgac cgcgttgtgg agaaattcgt gctcgagctt gaggcgcagc
    13981 tgatcgaccg tgacgtgacc ttcgatctga cgccggaagc aacccgctgg ctgggcgaga
    14041 agggctatga cgatgccttt ggtgcccgtc ctctcgcgcg cgtgattcag gaaaacatca
    14101 aaaagccgct ggcggacgaa atcctgttcg gcaagctgaa gaatggcggg acagtgcgtg
    14161 tgctgctcga ccgcgagaac gataagctcg ctttcgagtt tattacggat cagaagccgg
    14221 cttcgtccga aggcaagaag aagccgaaag aagaagtcta acgacgatgg aggtcaacat
    14281 ggccatgcag aacctgcgcg ccggtatggg cgcgatcgcg tgtggagcgg ccctcgtttt
    14341 ttctgctctt ccggccgccg cccatcatgg ctggggcggc aatgtggatc aggtgaccga
    14401 catgaccggt gtggtcgttt cgggggtgag ccttgccggg ccgcacggca ccatgaaagt
    14461 cagaaccacc gacggccatg tgtgggacat cacgctggcg ccgccgttcc gcaccagtga
    14521 ggcggggctg aagcagggta cgattcccgt tggcgccacc gttacggtgc gcggcaacag
    14581 gaacagcgat ccgaatcgct tcgagatgaa gacgatccgc gtcacctatg cgggcaaaca
    14641 atacgatacc tatcctgagc gcgaatcctg agcgcgaagc attgcggact tgagttttca
    14701 agtttcatga cgtggcttga gggatctgcc ctcggccacg ccatgcgcga ttccggtgtg
    14761 tggacctatg ggatcgtcaa tctcacgcat atcctcggcg tggcgaccct ctttggctcg
    14821 atcattattc tggatttgcg tcttctcgga ctgtggaagc acattccgct cagcgcgctt
    14881 tcccgcccca ccgtcgcgat ggcggccatc ggccttggcg tcgccgtcct cagcgggtcg
    14941 ggtctgctgg ccaccaaagc cacggaatat gtcggtaatc cgttcctcta tatcaaattc
    15001 cccgcgatcg cccttgggct gttcaatgtg tggctgctgc atcgttcggc tgcgtggcgg
    15061 gcgcacaggg ttcgcgccct cagcccgcgc gaggaatcgc aattgcgtgc gatggggggg
    15121 acctcgcttc tctgctggct gacggccatc agcgcaggcc gcatgatcgg ctattggtag
    15181 ccgcatgacc gcttagactg gatcgcggaa gtgcaggata tccggcatcg gatcgtagaa
    15241 atgatgcagc gcctcacgcc atttctggaa gcggtccgat tggcggaacc caaccatatg
    15301 gtcttcgagg ttttcccagc gcaccagcaa cagatatcgg ttcgcgtgtt cgatgcagga
    15361 ccgtacctcg agaccgacga aacctctggt cgcctcgatc aacggacgtg cgttcttcaa
    15421 agcagcttcg aaagcaggac cttgccccgg cttgatcgag agaatcgcgg attcgatgat
    15481 catggtgtca ctccagcgcg gctttgatcc tatcggcgag aagcccaagt ttttccggcg
    15541 tctccatctc gcgcgcgtgc agcttaaaat cggcgccgcc ctgtcgcggg atgacatgga
    15601 aatgcacatg gaacacactt tgacccgcgg atgcaccgtt caactgaaca atcatgatgc
    15661 ctggcgtgtt cagcgccttc ttcacggctt tggcaatttt ctgcgtcgtg gccatcaggg
    15721 cggccgcacc ggatgacgac aaatcgaaaa tattctcggc ggcctctttt ggaatcacca
    15781 gcgcatggcc gtccgcctgc ggcatcgcat ccatgaaggc gagggttttg ccgtcctcat
    15841 aaatcttcac cgcgggaatt tcgccgcgca ggatttttgc gaagatattg ttggaatcat
    15901 aggccatcgc attccccgat tgtgatgggc cgcaacggcg ggccatcggg cgatcatagg
    15961 actctttggg cgcatgacat gcaccggctt gatatctaaa gagtagtttt cttgtacggc
    16021 gcttgctcca tcaattcttc gatatgctcg gcaacggctt cgcgttcgtt cgccagataa
    16081 tcggcgaccg cccggcgcaa tccgggatgg gcgatgtaat gcgccgaata cgtgggcttc
    16141 ggcagatagc cgcgcagcaa cttgtggccg ccttgcgcgc ctgcttccac acgcgctagc
    16201 cccttggaaa tcgcaaagtc gatagcctga taatagcatg tctcgaaatg aagcagcggc
    16261 acataaccga ttgctcccca gttgcggcca taaagcgtga cgccatcgaa aaaattgagc
    16321 gcgcccgcga tgtaacgccc gtcgcgcttg gccatgacca gcaggatttg ctcgcccatg
    16381 ctcatcccca cgcggctgaa gaattcgcgc gtcagatagg gcgggttcca tttctgattc
    16441 ccggtcgtcg tatagcaaga gaagaattca tcccacacgg tctcggtcag gtcgcttccc
    16501 gtcagccatt cgaaggacac gccttcggcg agtatcgacg cacgctcctt gcgcatattc
    16561 ttccgtttag ccgatgacag atcgtcgagg aatgcgccga atgaatcata gccgcggttt
    16621 tcccaatgga attgcttatc ggtacggcgc agaaacccgc gcgtgcccgc aagatcccat
    16681 tcgcgttccg tcatgaacgt gatgtgcaga gaagacgcgc gaagctggtc cacggcgctt
    16741 tcggcggcgt ccagaagaat ttgccccgct tggatttcat tctcaccggc cgcgatcaac
    16801 aagcgccgcc cggtcgcggg ggtaaacggc acgcttgcct gcaatttggg ataatagcgg
    16861 ccgcccgcgc gctcgaatgc atcggcccag ccatggtcga agacatattc gccataggaa
    16921 tggctcttga gataaagcgg cagcagcgcg aacggtgtct catcgcgttc gagcaaaaga
    16981 tgcatcggcc gccaaccggt cttcgcagtc gccgagccgg agtcttccaa cgccgcgaag
    17041 aaggcgtggc gcgtgaacgg atgcggcagc gggcgccgtt cagggttggc cagaaaatcc
    17101 catgtgtcag cgccgatctc gtgcgcgctc gcacccaagc ctggtcttat ttttgtcacg
    17161 cgatttcgaa gatggcttcg atttccaccg cgacattgcg gggcagcgac gaaacgccaa
    17221 cggccgcacg cgcatgcttt ccggcatcgc cgaaaatgtc cgcgaaaagt tccgacgcgc
    17281 cgttcaccac ctcggggtgc tgcgtgaagc cgggtacggc gttgacgaag ccggtgactt
    17341 tcaccacccg gctgacgcga tcgagcgcgc cgtcgagcgc ggcggcagcc tgcgcaatca
    17401 cattgatggc cgccaatcgc gccgcggctt tagcgtcttc caggttgatt tcccggccga
    17461 ccgtgcccac atattccaga ccgcgcgcac ccatcgtgat ctggcccgag atgaaaagca
    17521 gcgcgccgct tttcacccat ggcacataag caccttgggg cgcagccgga accggcagcg
    17581 tgattccgct ttcgatcaat tttgccgcga tgcgtccggt cattttgtcc tcttttcgca
    17641 tcaaactaac gtgccatcgt cttcgatgcg agggggctcc acagtgtgac cgaaaaatgg
    17701 ttaggctatc ccccaatatg gtggacgcat gaatagaact ctcgatgccg cttcgccttc
    17761 gcccttggag gatggctacg tctttcccgc cgagtggcac ccgcaccggc gcacctggat
    17821 gtgctggccg gtgcgcaccg cgtgtttcgg gggggcggac ggcgttttgc gcgcaaaaca
    17881 ggcctatgcg cgtgttgcgc gcgcgatctc cgcttttgag cctgtcaggc ttgccgcccg
    17941 gacacaggag gtcgctgagg cgcggttcgc caccggaggc aaggtggaag tcgtggaaat
    18001 ggcgctggac gattcctggg cacgcgattt cggcccgaca ttcctcaaga acgacagcgg
    18061 aaggctcgcc ggcgtccaat ggatcttcaa tgcgtggggg cggaagtatc atccgtatac
    18121 gaatgatgag acctttgcgc gccgggtttt ggagatgatc ggcgccgacg tttacgccgc
    18181 gccgcttgtt tgcgagggcg gcgcgcttca tgccgacggc gagggcacgg tgctcaccac
    18241 ggagcaatgc ctgctgcatc ccaaccgaaa tccgggccgc acgcgggaag aggtggaaga
    18301 tgtcttgaaa tcccatctgg gcgcacgaac ggtggtctgg ctgggctccg gcttctccga
    18361 taccgagacc gacggccata tcgataatat cgcctgtttc gccgctccag gccgcgtcat
    18421 tctcgggatg cccgcggcac ggagccatcc cgactacgca cccgtgcagg aagccatgtc
    18481 gcggctcaag gcggcccgcg atgctgaggg ccgggccttc gagatcgttc ccctgccgca
    18541 acccagaaag catcgtcaat cctggtccgg acggccgctt gccatgagct atgtgaattt
    18601 ctatctcgcc aacggcgcgg tcatcatgcc ggccttcggc gatccgaacg atgaagatgc
    18661 acgcaccatt ctggcccaat gttttcctgg gcgcgacatc atgcagatcg acgcgctcga
    18721 tatcgttcag ggcggcggcg gcattcactg catcacccag caggaaccgg cggcgtgagc
    18781 accgtcacgc tcgccgcggt acaattcgcg tgcacctggg acctggcggg caacatcgcg
    18841 aaagccaagg ggatggtgcg gcgcgcggcg gcggcaggcg cgcagatcgt tctgctgccc
    18901 gaacttttcg aaacacccta tttctgtcag gaccagtcgg caaagcattt cgcgctggcg
    18961 cggagctttg aaggcaatcc catcattgcg gagttcgcgc gtctggcggc ggagctttcc
    19021 gtcgttcttc cgctgagctt tttcgagcgt gcccacaatg cccattacaa tgcgctcgcc
    19081 atgatcgatg ccgatggccg cgttctcggc cgctatcgca aatcgcacat tcccgatggt
    19141 ccgggttatc aggagaaatt ctattttcgt ccaggcgata cgggtttcca ggtttggaag
    19201 acccgccatg gcgaactcgg cgcagggata tgctgggacc agtggtttcc ggaaagcgcg
    19261 cggatcatgg cgctgaaggg cgcggagatt ctcttctatc ccacggccat cggaagcgaa
    19321 ccggcacccg cgccgccggt ggattcgcaa gcccattggc ggcgtgtgat gcagggtcac
    19381 gcggctgcga attgcgtacc gctggtggcg tcgaaccggg tcggcgccga acggggagag
    19441 gcgggcgaga tcatttttta cggttcatcc ttcattgccg gcccgacagg agagattctt
    19501 gccgagatgg gacgagagga agaagggatc attgtcgcgg atttcgatct tgccgcgatc
    19561 gccaaggcgc gtgcgtcgtg gggcttgttc cgcgaccggc ggcccgatct ttatggaccg
    19621 atcctgaccg ccgacggaga gacgtgaccc gcgaccggcg gcccacgata tgagcggcgt
    19681 ttttgcgcgc ggtgacgggc aaggctaagg ttgcgtccgc agtttttccc aacaaatgaa
    19741 accctgttca ggagggggcg acgatgcatg caggtcgaac agcaattgtc cttttagccg
    19801 gcgccgctct gctggccggg gccgcaacat cggcatgcgc ccagcagaat ccggccccaa
    19861 aaccgaccga tcttcccaat ccttatcgcc tcgtggaggg ctggccgacg ctgcccaaaa
    19921 gcatgaacgg cggtcattgg ggtgaggtta tccgcgtcca tgtggagcgc gacggcaata
    19981 tctgggtctt tcaccgctgc ttcaacacgg tgccgcccgg cagtgcggtc tgcatcaatc
    20041 gtgggccggc caatcctccg atcctggaat tcgatccctc cggcaagctc ttgaagagtt
    20101 tcggggcagg tctgttcgcc tatccgcatg ggttcactgt ggatcaagac ggaaacctgt
    20161 gggtaacgga cgtcaacgac gccgagacag tgctcggcat gtcggccaga aacgccgccg
    20221 gggtcgtcat ggggcaggag gtgctgaagc tcagtcccac gggcaaggtg ctgatgacac
    20281 tcggcaagga aggggtgtcc ggaaacggaa tcgacacgtt cgaccggccc accggcgtcg
    20341 ccatcgcgcc caatggcgat atcttcgtga ccgatggcca cttgcccaac gcgcacaaca
    20401 atggccgggt gctcaaattc tcgaaggacg gcaaattcat caagcaatgg ggccggaaag
    20461 gctctgcgcc cggagagttc gacgagcccc acgatatctt tctcggcggt tcgcaaaacc
    20521 gcatctatat cgccgaccgc aggaaccagc gcatccaggt gttcgatcaa gagggcaact
    20581 tcatcgcggc ctggaagcaa ttcggagagc cgagttcggt gttcgtaggc cgggacgaca
    20641 ccatctatgt ggggtccgga tttcccgacc cgacagccaa aagaggcgag atcaggggca
    20701 tcgtcgtcgg caacgccaaa gacggctcgc tgaaggcatt cattcccgat cccgcagacc
    20761 cgaacagtct gagccgtggg acaacctcgt ccgggattgc ggccgacgac gaaggcagca
    20821 tctattccgc cgacgtcgcc gcgcataatt tgcgcaaata cgtcaaggtt aaatagagcc
    20881 gttaccaggg ccctgggtta gaccgctcaa ccggaagcaa gccgtcaggc ggcggcctat
    20941 caccggaaca atgctccggt cccgccgaaa gaaccggtcc cgttaaccgt cgggcgcgac
    21001 accgggaagc gcccggcggg cggcatgaag catcggggaa tcgaggtctt cagccgcgag
    21061 aaatcccgta cagagcgctt cgtccgaaag aagaaagtcg atcacgccgg caagcagctc
    21121 cggattgccc gcccgcgcgc gcagatcatc gaattcaagc ccggtgagcg tcaggaatcg
    21181 tatcagcccg tccccgtccg ccgccagata cgcgagagct ttcaaggcca gtgtttcggc
    21241 tgccgagcgc tgcaatcgtc aatccgccgt taagacattg agcataaaac taaccatcgg
    21301 cgaaatgagc gctgtgcgag tggtgtcccc cctcacgctc aaaagcaagg accgggcgcc
    21361 attcgggcgg catcgggtat ggagtgtcaa gcgatggatg cctcggcgaa aacggttctc
    21421 attgtggaag acaatgagct caatatgaag ctcttccacg atcttctcga tgcccatggc
    21481 tatcgcatcc tgcagacgaa ggatggcatg gaagccctgg atattgcgcg cgagcatcgc
    21541 cccgacctca tcctcatgga tatccagctt cccgaagtgt ctggacttga agtcacaaaa
    21601 tggctgaagg aagacgattc cttgcgcgcg atccccgtcg ttgcggtcac tgcctttgcc
    21661 atgaagggcg atgaggaggt gattcgccag ggcggctgcg aggcttatat ttcaaagcct
    21721 atttcggtca ccagttttct cgaaaccgtc cgccggttcc tgggctgacg cgatagcgat
    21781 taccaatgac cgctcgcgtt cttgtcgtcg acgatatcct atccaacgtc aaattgttgg
    21841 aagcgaagct cgccgccgaa tatttcgaag tcatcacggc cagcaacggt gagcaagcgt
    21901 tgacgcgcgt cgccgccgag ttgccggata tcgtgctcct ggatgtgatg atgccgggaa
    21961 tggacggttt cgaggtttgc cggcgcatca aagccaacag caaaacgacc catattccga
    22021 tcatcatggt gacggctctc gatcaaccga gcgaccgcgt gacgggattg catgccggcg
    22081 ccgacgattt cctcacgaag ccggtagacg acacggctct gttcgcgcgc gtgcgttcgc
    22141 tggtgcgtct gaaaatgatg accgacgagc tacggatgcg cgaagagacc ggccaaagca
    22201 tggggttggc cgacgctggc gaaaccctgt tcgaagccga cccgacggga catattctga
    22261 tcgtcgagga ccggccggaa tcggtggcct ggttccgcgc cgttctcgaa accaagaaca
    22321 aggtcgccga tgcccgcaat tttgatgagg cgttgttgct ctcgcgcggc ggcgatttcg
    22381 atctgatcgt cgtgagtctc ggcatccggg cctttgacgg cctccggctg tgctcgcatt
    22441 tgcgttcgct cgccgagatc cgcaatacgc cgatcctggt tctcgtgagc gatggcgaaa
    22501 atcgcaaact cgcccaggcg ctcgatatgg gcgtcaacga ctatctgatg cgccctgtcg
    22561 atcgcaatga gctaatggcg cgggtcaaga cgcagcttcg aaggaagcgc tatgccgata
    22621 aattgcggca taatatgcag ctcagcctgg aaatggcgat caccgatcag ctcaccggct
    22681 tgaacaatcg ccgctacatg gcgcgtcatc tcgaaacgct gcttaagaat gccagcgaaa
    22741 cgaagccgat ctcgtttctc atcatggata tcgattactt caagacggtc aacgacacgc
    22801 acggccatga tgtcggcgat gaagtcctgc gcgagttcgc ggcgcggatt tctgcaaatg
    22861 tgcggggtat cgatctggcc tgccgttatg gcggtgagga attcgtggtg gtgatgcccg
    22921 acaccgacaa ggaatttgcc cattccgtcg ccgagcgcct gcgccatacc atggaattga
    22981 tgcccattcc gatcagccgc gcgccgaaca agatcgtcat taccgtcagc atcggcatcg
    23041 catcctcgat aggcggcgac gacaattccg acaagcttct gcatcgcgcg gatcaggcgc
    23101 tgtatcgcgc caagcgcgag ggacgcaatc gcatcgccga agcggcctag ccgaatccca
    23161 cgcgtatcgg gttattgggg cccggctatt gggccgggct attgggcgac gggcgcggag
    23221 atttcgttgg tcgccgaaac gatacggatg gttttcacct ccggtgccag caagtccacg
    23281 cgcagttcag cctctattgt ttggaccgtc cgattttcgt cgggcatcgc gggcgaggta
    23341 gcaaccagcc tgtaggtctt gatgctggca tccccgccat tgtccggctc ctccgcgagc
    23401 ttcgcgtcgg tccagcccgg cgacggagcc gttcccgaga cctgaatcac aagcgccatt
    23461 ggtccgtccg gtggccggct cagcatgacg ctgtccacgc tttttagggg aagttcggtt
    23521 gacgcaggga tcggcggcgg cgggggaggc ggtgcgggaa gaggcgccgc gggagccgcg
    23581 ggcgccggtg caggcggcgc cgggttttgc tgagaattgc gattgcaccc gctcagcgca
    23641 atcgcgagga ccacgatgag cgcaatcttg tgacgaaacg aatccatgga atgcgatttc
    23701 cccgggcgag caccttaccc aatatcgcat ttccacatac gaaggcaccc aggcgggtca
    23761 atcgacctac ccgggtgcca aaaaactcag ccggctacgg ggccggtgct cctggggcgc
    23821 caggtgctcc aggcgcgccg ggcggtcctt gatccggtgc gggggtgggt tcgccaggcg
    23881 ctccgggtgc ccctggagcc cccgggggac cttgctcggg agccggagcc ggcgtcgcag
    23941 cgggcgcagg ctctcctgcc ggagtcgggg caggattgtt ctggttgcac ccgatcagaa
    24001 ccgcggtcgc agccgacagg agcaacatat tgaaaacttt ggctttcata gggttccctc
    24061 ttatagctct ctggcgcggc gcggcgaagt tcttccgcgc cgacaggagg gaacgcacag
    24121 tcggccaaga agttgtaagt tttctcacag gcgagtgtct gccagggaac tctaccaggg
    24181 gaactgcagg ggggcagata tctgttcgca ggcagaggcg tagcctactg cccgtgcccc
    24241 cttactggga agcaagtagc ccggatatga ttggcgtcaa attcaattca cgccgaacag
    24301 caggccgaac acagcaaaaa caccccggcc ggatatggcg gccggggtgc cgcgtattaa
    24361 gaaattgtcc ctggcttacg gagcgggggc agcaggtgcc ggctctgccg gagcgggttc
    24421 cgccggggcg ggttctgccg ccggaggcgc aggctctgcc ggcgctgcgg caggggcttc
    24481 ggctggtgcc gccgccggag caggcgcgga attctgattg caagcaacca gcaacgtcgc
    24541 gattgcgagc gcagcaaggg ttttcagaga cttcattttt gtaactccta ttcaggcaga
    24601 aaaatgaatg cgggaatcga gattggagtc tattgagtaa tcgagacgca ggtcaaactc
    24661 aacgctcaaa tagccaattc gcacagaaca ggaacatggt ccgagggttt ttcccaccct
    24721 ctcaccttgc gttctatcga acagctgacc agtcggtcgg cagcctgggg cgataataga
    24781 atatgatcga ttcggatgcc atggtcttta acccatgcgc cggcttgata atcccagaat
    24841 gtatagcaac ggggttcggc gtggcaggcg cggaaggcgt cggtcaggcc gagatgcaag
    24901 atgcgacgga acgccgcgcg ggtttccggc tggaaaagcg cgtccccggc ccatgccttc
    24961 gggtcgttac aatcgtccgg ggccggaatg acgttgtagt cgccggtgag aatcagcggt
    25021 tcctcgaagg tcaggagatg gcgaatatgc cggttcagac gctccaacca ggcgagcttg
    25081 taggcgaatc gctccgtacc cggcgggttg ccgttgggcg catagatcga agcgacgcgc
    25141 acgatgccgc cggattcgcc gggaacgacc gcttcgagat agcgcgcatg atcgtcaccc
    25201 tccacggcgg gaagccctct ggtgagatcc tccggcggtc ttttcgaaag gatggcgacg
    25261 ccattgtagc ttttttgtcc gtgcacggtg caattgtagc caagagcttc gaagatctcg
    25321 ctcgggaagg tgccatcggc ggtcttgatc tcctggagac agacaatgtc gggcgcggcc
    25381 tcggcgagcc atgtcgtcac ggcgtccagc cgcgctttga ccgaattgac gttccaggtg
    25441 gcgattttca tttgcgcgcg gctaaatcga gaaactcgta ccgcagccgc aggacgatgt
    25501 cgcattcgga ttgttgatct tgaacgcggc tccgatcatt tcctcgacga aatcgatctc
    25561 ggcgcctttg aggaaatcga gcgaaaccga atcgatgagc accctggcgc cgtcttgggc
    25621 gatcagaaga tcgtcctcgg cgcgcgaatc atcgaagcag aaatcatact ggaagcccga
    25681 gcagccgccg cccgaaacgg cgacgcgcag gttcgacgct ccggtttctt cccgcaggat
    25741 ctcggcgatc cgcttggcgg cccgatcggt cagacggaca ggaggggatt ggatcgtttc
    25801 aacgctcatg gagaacagcc tatcatgccc ctgctatgta atctttgcag ggccgatgtc
    25861 caagacgcca ccctcaggcc gccttttggg gccgcttttc gtccctccgc cgcgggcgcc
    25921 ctatgccacg aatcccgcgt ccagccgggg ccgcttctgg gccgaggagg aaagcgccac
    25981 ccgcagttgt taccagcgcg accgcgacag gatcatccac tccacggcgt ttcgccgcct
    26041 caagcacaag acccaggtct tcgtccagca tgaaggcgac tattaccgga cccggctcac
    26101 ccattccctc gaggtggcgc agatcgcgcg ttcgctcgcg cgcgtgctgg ggctggacga
    26161 agacctcgcc gaggcgctcg cgctggccca tgatttgggt catccacctt ttggccatgc
    26221 cggagaagcc gcgctcaatg cttgcatggc gggccatgac ggcttcgacc acaacgccca
    26281 ggccctcgtg ctcgtgacac ggctggagca tcgctatgcc gatttcgatg ggcttaacct
    26341 cacctgggaa acgctggagg gccttgtcaa gcacaatggt ccgctgctgc acgaaggcgc
    26401 cggtcttcgt gaatctctgc ctgaggcgat cgcggccttt ccttgggacc tcgagctttt
    26461 cacgcatgca ggccccgagg cccagatcgc ggcgttgtcg gacgatatcg cctacgtcaa
    26521 tcacgacatc gacgatggtt tgcgcgcggg cctcttccag atcgaggagc tcttcgaagt
    26581 cgcgctggtg ggcccgatct ttagggaact ttccgccgcg catcccggtc ttgagcgcgg
    26641 caggctgatc ggcgaggcga tccgaaggct catcggtcag atggtgggcg atgtgacggc
    26701 ccagaccctg cgtgccctcg gagcgcttgc gccgcggaac gcgggagaaa tccgcggcta
    26761 tggcacgcct ctcgcggaat tttcgcccgc tttgcgcgcc gatctctcga aactcaagga
    26821 atttctcttc cgccggatgt accatcatcc tcgcgtgctg caggtgatgc agaatgctca
    26881 gctcgcgctt acgcaattgt tcggaacgtt catggccgac ccggctctgc tacccaggga
    26941 ttggatggcc ggttgcggcg cacccggtga tagcgtgacc gcgcgggccg tgtgcgatta
    27001 cattgccggc atgacggatc gtttcgcctt gcaggaacat cgcaaattct tccacatgga
    27061 aattgcgtta tgagcggtag aagggtcttg gcgtgcggtg cttcgaaggc gcacgggtga
    27121 taaaaatgca gaacatgcga ttcggtggag ccggcccatg aacacacatc aacgcggcgt
    27181 ctatcagccg gcaatggaca atgctcaggt ctacgatctt tccaacgagg aggtcgaaga
    27241 agaggagcat tcgcgcctgc cgcttctgat cgtcatagcg cttctggtgt tggctgcctt
    27301 cgccggtgtg gtgtggcttg cgtataatca gggcgtggcg cgcggccggg cgggggcggt
    27361 ggttgtcatc gccgcaccgg atgggccggt tcgcaccgcc ccgctggacc ccggcggtac
    27421 gcctgcaccc tatacgggcc tgaaagtcta tgggcagtcc ttacccccgg accaggaggc
    27481 ccaagggact acgcttgcgc cgcaggcccc agccggcatt ccggcttcat ctcccgccca
    27541 gaggccccaa cccgcgcccg cggtggtgac atccgatacg ccgccggtga ggctaaaccc
    27601 agacgccccc acgctgacgg cttccaaacc gccgccgcca gccgctgtca aaccggcggt
    27661 tcagaccgcc gccgccactt cggccgctgc tgtcgcggcc gccgtcccgt cgcaatctgc
    27721 cgctctgacg tccgccgctt cgggcaaggc ggtgttgcag atcggcgcct atgaaagtcc
    27781 cgagatcgcc aacggagcct ggacctcttt caaatcccgt tatgccgatg tcgctgcaaa
    27841 actcggcgaa gacgttcaaa aggccgacct cggcgccaag ggcacgtggt atcgcctgcg
    27901 cgttggtccc ttcgccgaca aagccgccgc
//
LOCUS       DASYEG010000285         9055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1559885, whole genome shotgun sequence.
ACCESSION   DASYEG010000285 DASYEG010000000
VERSION     DASYEG010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 9055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9055
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559885"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VGM72_12210"
     CDS             <1..190
                     /locus_tag="VGM72_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266075.1"
                     /translation="FGMLPLFEYGKTVVLAGNDAGLRQIPVLAADAGTITYTNVAKLD
                     DTIRPCAQGRPARTRRRL"
     gene            complement(280..1077)
                     /locus_tag="VGM72_12215"
     CDS             complement(280..1077)
                     /locus_tag="VGM72_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266076.1"
                     /translation="MAVLIAAIAASAAAAQSPDPPDFSSATAGWLTPIAGDFAAVPGS
                     PGPIVSDPAHPFLNNVEARERGITPSYRIADLSNPNLKSETKGAMKKDIDEVLAGKIA
                     YSPSTSCTPAGVPAFLLQPSVLYFVQTPKEVVMIQSYDSQVRHVYLNVPHSARITPSW
                     YGESVGHYEGDTLVVDTVGLNGKTFVDAYRTPHSEKLHVVERWKLAGGGNGLEVTVEV
                     DDPETYNRPWKAVLRYRRSPGAVLEAICAENNLQFDYHMPMAERAEF"
     gene            complement(1198..2004)
                     /locus_tag="VGM72_12220"
     CDS             complement(1198..2004)
                     /locus_tag="VGM72_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266077.1"
                     /translation="MVTKAAIVTLAIGAAYAERFEQLCRRNWTAYAERHGFDLVIIKQ
                     PLDTTERAQRRSPAWQKCLILDAPGVADYERVVWVDSDVLLNPASPSILDGVPVEQVG
                     VTDEHVYPTPEARQEILRAVVASAPDHGALGGKSYWAKWHEPGPWHEAMGLPGGQGHI
                     VQTGVMVLSPRHHRDMLEHVYRAYEDGGLNYEMRFLSHEILARDLAHWIDPRFNALLW
                     WLFLQANMGRGRVTTPREIQDFVMESYRRNYFLHFAGAANLMPLADPAGG"
     gene            2247..3116
                     /locus_tag="VGM72_12225"
     CDS             2247..3116
                     /locus_tag="VGM72_12225"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY4266078.1"
                     /translation="MNPNPPSPPLWKASATVAKDAAANIAALVELAPPAPQAVLIVED
                     PFGPESTVEALYTAPPDSGFLTRLTGHEVTVAPLPDQDWIRLSQEGLPPVRSGRFFIH
                     GAHDAGVVPPGVIPIRIEAGLAFGTGHHETTALCLEALCDVARSERPRAVLDVGCGTG
                     VLAIAAAKLWPRTVIATDIDAVAIEVARENARINDCGPLILTAVADGLTHPAIRTHAP
                     FDLIFANILAGPLTKLAPSLSSALARRGITILSGLLRNQEIQVLSFYRPHGLVLRRIY
                     RDGPWSALVLERP"
     gene            3132..4958
                     /locus_tag="VGM72_12230"
     CDS             3132..4958
                     /locus_tag="VGM72_12230"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEY4266079.1"
                     /translation="MNMPFQTFDEESDASQCAPRLAALRAGLAVRGVQGFLVPRADEH
                     QGEYVPKRAERLAWLTGFTGSAGAAVVLIEKAAIFVDGRYTLQVRNQTDTNLFETLDI
                     MNDGLANWIEANLPQGARLGYDPWLHTQGAVERLKQAVERAGGQLVALESNPLDAVWP
                     DQPGPPRAHAVPHAIELAGETSVSKRSRLAENLKKRGADAAVLTMPDSVCWLLNIRGA
                     DVPHTPFALAFAILNADSSVDLFLDAKKRSPELLEHLGNEVHLRAPEDFGPALDALKG
                     KTALADPVWAASAIFQRLEKAGARIIRGADLCQLPKACKNAAELDGTRRAHIRDGQAL
                     TRFLAWFAAEAPKGSLTEIDTVEKLESFRAATGALKDVSFDSISGAGPHGAIVHYRVT
                     KKTNRRIENGQLFLIDSGAQFVDGTTDVTRTVAVGAPSAEMRDRFTRVLKGHIQLALA
                     RFPVGTTGAQLDAFARRPLWEAGLDYGHGTGHGVGSYLSVHEGPQSISSRGTVQALKP
                     GMICSNEPGYYKTGEYGIRIENLVVVREPAPIADGEKPMLGFETITLAPIDLHCVEPA
                     MLTPDETRWLNAYHARVREAVAPAFAGDEKGRLWLEQATRAI"
     gene            complement(4955..5122)
                     /locus_tag="VGM72_12235"
     CDS             complement(4955..5122)
                     /locus_tag="VGM72_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266080.1"
                     /translation="MNQIVIRNLDDEVLQSLKQIAWQDGRPPAEMAQRLLIEAVRSRA
                     VRRRFAVLEPH"
     gene            complement(5183..7354)
                     /gene="ligA"
                     /locus_tag="VGM72_12240"
     CDS             complement(5183..7354)
                     /gene="ligA"
                     /locus_tag="VGM72_12240"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY4266081.1"
                     /translation="MSAANSKSPDQLTENEAKTELARLAHEIAEHDRRYYQDDAPSIT
                     DAEYDALRRRNDEIEKRFPELIRHDSPSQRVGAKPAERFEKVRHARPMLSLDNAFTDE
                     DVRDFVARVRRFLALPEDQELMLVAEPKIDGLSSSLRYEKGELVLGATRGDGAEGENV
                     TANIRTVSDVPKKFHGRHPPEIFEVRGEVYMSHADFAALNRRQEKAGDKIFANPRNAA
                     AGSLRQLDPEITARRPLRFFAYHWGEASELPGATHWEVLQTFKGWGFPVNPLVSRCRN
                     VEEALAFYEEVQHARADLGYDIDGVVYKVDRLDLQNRLGFVSRSPRWAIAHKFPAEQA
                     ESVVERIAVYVGRTGALTPVAHIKPVTVGGVVVQNVSMHNEDEVARKDVRVGDTVVVQ
                     RAGDVIPQLVRVVPERRPKGTKPYHMPEKCPVCGSHAVREINPKTGAPESARRCVNTL
                     SCPAQVVERLYHFVSRSAFDVEGLGGEFIQLLHEADLVKEPADLFTLKNHPEAFHAVL
                     TRHFAAISERNAVKRGKEAAAKKAKAQKTEYLLGNKLLAAIEARRVISLDRFILSLSI
                     PHVGETTAKLLARNFHTAKTFLDSMESDSAVTDLDAIEGIGDVMAGAIKNFFDDRHTR
                     DAVARLMPHIEVTDVAPHTAADSPVAGKTIVFTGTLEKMSRNEAKARAESLGAKVAGS
                     VSKKTDLVVAGPGAGSKLKNAQEFNVTIISEDEWLQLIGAG"
     gene            complement(7351..>9055)
                     /gene="recN"
                     /locus_tag="VGM72_12245"
     CDS             complement(7351..>9055)
                     /gene="recN"
                     /locus_tag="VGM72_12245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEY4266082.1"
                     /translation="KQVMLASLTVRDIVLIEKAELHFAPGLNVLTGETGAGKSILLDA
                     LSLAAGERGVGRAAIRAGAEAATVVAVFDVAPDHPAHVLLKANGFDAEANDILLRRAV
                     TADGRSRAYVNDSPVGIQLAREIGSLLLEIHGQTDDRGLFDVATHRLLLDAFGGHTLL
                     AEEVVRCHAALVATTGRLASLRRAQAAAAEEIEYLEYAAKELRDLAPEPGEEEPLATT
                     RGLLTNAGRLAQEISAAADFVGGDASAEVQLAAALRRLSRLPAEGRSAVSAAETALDS
                     ALTLVQEARSTLEVLLRQLENDPGRLEIVEDRLFALRAAARKYNVPVDSLGRLRDEFE
                     AKLASLDVSGTAITAATVEVERLRADYRARARELSACRAAAAKNLEEAVSAEFSPLKL
                     GAARFRVAFAPLDEGTAGASGFERIAFEIATIEGASFGPLAKIASGGELARFALALKV
                     SLAAVSPPAVLVFDEVDRGVGGAVAAAVGERLQRLAQTTQVLLVTHSPQVAARATRHF
                     RIARQKDATRVSELDIEERVEEIARMLSGAAVSDEARAAARRLVAESQAQSKPKKRVR
                     A"
BASE COUNT         1654 a         2929 c         2748 g         1724 t
ORIGIN      
        1 attcggcatg ctgcccctgt tcgaatacgg caaaaccgtc gtgctcgccg gcaatgatgc
       61 gggattgcgg caaattccgg ttttggccgc tgatgccggt accatcacct ataccaacgt
      121 ggccaagctc gacgatacga tcaggccctg tgcacagggg aggcccgcaa ggactcggcg
      181 gcggctctga cgcaaatttt tcgtgactac agcgcgcgcg ttcaactggt gtcgaattga
      241 tcgccggggg atcgtctgtg acgctccgga gggccgtgct cagaactccg ctctctccgc
      301 catcggcata tgataatcga actgcagatt gttctcggcg cagatcgctt ccagcacggc
      361 tcccggactt cgccgatagc gcaaaaccgc tttccagggc cgattgtagg tctcgggatc
      421 gtccacttcg accgtgacct ccaatccgtt gcccccgccg gcgagtttcc agcgctcgac
      481 gacatggagt ttctcgctgt gcggcgtgcg ataggcgtct acgaaggttt ttccgttgag
      541 accgacggta tctacgacaa gcgtatcgcc ttcataatgt ccgaccgatt ctccgtacca
      601 ggacggtgtt atcctggccg aatgggggac gttgagatag acatggcgca cttgtgaatc
      661 gtaggactgg atcatcacca cttccttggg cgtctgcaca aaatacagca cgctcggttg
      721 cagcaggaag gcgggcacgc ccgcgggcgt gcaggacgtg gagggggaat aggcgatttt
      781 tccggccaag acttcgtcga tgtccttctt catcgcgccc ttcgtctctg acttcaaatt
      841 ggggttggaa agatcggcga tgcgatagct cggcgtgatg ccgcgctccc tggcctcgac
      901 attgttgagg aaagggtgcg ccggatcgct gacaatggga ccgggcgatc ccgggactgc
      961 cgcgaaatcg cccgcaatgg gtgtgagcca ccccgcagtc gcggaggaaa aatccggcgg
     1021 atcggggctt tgcgccgccg ctgccgaggc tgcaatcgcc gcaatcagaa ctgccaaatg
     1081 tctgagcaaa aacggcccgt gaatccccaa aacggaagcc tccaacattt ccccgaatgc
     1141 taatacggtt ccgccaagga ttgcagcttt atccggcgaa attccgggac cgcgtctcta
     1201 accgccggcg ggatcggcca gcggcatgag attggcggcg cccgcgaagt gcaggaaata
     1261 attgcgccga tagctctcca tgacgaaatc ttgaatctcc cgcggcgtcg tcacgcggcc
     1321 ccttcccatg ttcgcctgca ggaacagcca ccagagaagc gcattgaacc ttggatcgat
     1381 ccaatgcgcg agatcacgcg ccaagatctc gtgcgagagg aatcgcatct cgtaattcag
     1441 gccgccgtct tcatacgcgc gatagacatg ctccagcatg tcacgatgat gccgaggcga
     1501 gagcaccatg acgcccgttt gaacgatgtg cccttggcct cccggcaatc ccatggcttc
     1561 gtgccacggc ccgggctcat gccatttcgc ccaataggat ttgccgccca acgcgccatg
     1621 gtccggcgcg ctcgccacga ccgcgcgcag gatttcctgc cgtgcctcgg gcgtcggata
     1681 gacgtgctcg tcggtgacgc caacctgttc gaccggcacg ccgtccagga tcgacggtga
     1741 cgcgggattg agaaggacgt cggaatcgac ccacacgacg cgctcataat cggcgacacc
     1801 gggcgcgtcc agaatcaggc atttctgcca ggcggggctg cgcctttgcg cccgctcggt
     1861 cgtatccagc ggctgtttga tgatcacgag atcgaagccg tggcgttcgg cataggcggt
     1921 ccaattgcgg cggcacaatt gctcgaaacg ctccgcatag gccgccccga tggcaagcgt
     1981 gacgatggcg gccttcgtca ccatgccggc cttcccaaag aaaattcgcg attcgtctca
     2041 atgcccatgg tgttccgcag cgtgcactcc gatatccatg caatgatcct tgttctcaag
     2101 accagtgccg cgatgctgat gggaccccaa ttgcaccttt attgcacctc tattcgcacc
     2161 aaattttatg gcgcggtgac ggcttttttc gcggcaaagc tggatttttt caccgaccct
     2221 gcgtcatttt cggggggagg gggggtatga acccgaaccc tccctcgccg cccctctgga
     2281 aagcctcggc cacggtcgcc aaggacgccg ccgccaatat cgcggcgctc gtggaattgg
     2341 ccccacccgc gccccaggcg gtcctgatcg tggaggaccc gttcgggccg gaatcgaccg
     2401 tggaggcgct ttacaccgca cctcccgatt cgggtttcct gacgcggctc accggccatg
     2461 aagtcactgt cgcccccctg cccgatcagg attggattcg cctgagccag gaaggcctgc
     2521 cgccggtccg cagcggacgc ttctttattc atggcgccca tgatgcgggt gtggtcccgc
     2581 cgggggtcat tcccatccgc atcgaagcgg ggctcgcctt cggcaccggc catcacgaaa
     2641 caacggcctt gtgtttggaa gcactgtgcg atgtcgcgcg cagcgagcgg ccgcgcgccg
     2701 tgctcgatgt tggttgcggc accggggtgc tcgccatcgc cgccgcgaaa ttgtggcccc
     2761 ggaccgtcat cgccaccgac atcgacgcgg tcgccatcga ggtcgcacgc gagaacgcac
     2821 gcatcaacga ttgcggtccg ctgatcttga ccgccgtcgc cgatggcttg acgcatccgg
     2881 cgatccgcac ccacgcgccc ttcgatctca tcttcgccaa tatcctggcc ggccccctga
     2941 caaagctcgc ccccagcctt tcgtctgcgc tcgcgcggcg cgggattacg attctgtcgg
     3001 gcctgttgcg caatcaggaa atccaggtgc tgagcttcta tcgcccgcat ggactggtgc
     3061 ttcgtcgcat ttatcgcgac gggccgtggt cggcgctggt cctggaacgc ccataacgct
     3121 aggtttattc catgaacatg ccctttcaaa ccttcgatga agaaagcgac gcctcgcaat
     3181 gcgcgccccg gctggccgcg cttcgcgccg gactggcggt gcgcggcgta cagggttttc
     3241 tggttccgcg cgccgacgag catcagggcg aatatgtccc caagcgggcc gaacggctcg
     3301 cctggctcac cgggttcacc ggctctgccg gcgcggctgt cgtgctgatc gagaaagccg
     3361 ccatctttgt cgatgggcgc tacacgctgc aggtgcgcaa ccagaccgac acgaatctgt
     3421 tcgaaacgct cgacatcatg aatgacgggc tggccaattg gatcgaggcc aatctgcccc
     3481 aaggtgcgcg cctaggctac gacccgtggc tgcatacgca aggcgcggtc gagcggctga
     3541 aacaggcggt cgaacgtgcg ggcggtcaac tcgttgcgct ggaatccaat ccgttggatg
     3601 cggtgtggcc cgatcagccc ggaccgccgc gcgcccacgc ggtgccgcac gccatcgaac
     3661 tcgcgggcga aacctccgtt tccaagcgaa gccggctggc cgaaaatctg aagaagcgcg
     3721 gcgccgatgc ggcggtcctg accatgccgg actccgtctg ctggctgctc aatattcgcg
     3781 gtgccgatgt accgcacacg ccctttgcgc tcgcctttgc gatcctcaac gcggattcgt
     3841 cagtggattt gttcctggat gcgaagaagc gctcgcccga attgctggag catctgggca
     3901 atgaggtgca tttgcgcgcg ccggaggatt tcggccccgc cttggatgca ctgaaaggca
     3961 agaccgcgct ggccgatccg gtgtgggccg catctgcgat tttccagcgg ctggagaaag
     4021 ccggggccag gatcatccgc ggtgccgatc tgtgccaatt gcccaaggcc tgcaagaacg
     4081 ccgccgaact cgatggcacg cgccgtgccc atattcgcga cggccaggca ttgacgcgct
     4141 ttctcgcatg gttcgcggcc gaggcgccaa agggcagcct cactgaaatc gatacggtgg
     4201 agaaactcga atccttccgt gcggcgaccg gcgcgctcaa ggatgtgagc ttcgattcca
     4261 tctcaggcgc aggtccgcac ggtgccatcg tgcattatcg cgtgaccaag aagacgaacc
     4321 gccgcatcga gaacgggcaa ttgttcctga tcgattccgg cgcgcagttc gtggacggca
     4381 cgaccgatgt cacacggacc gttgcggtgg gtgcgccatc cgcggaaatg cgcgaccgct
     4441 tcacgcgcgt actgaagggc cacatccagc ttgcgctggc gcgttttccc gtgggcacga
     4501 ccggcgcaca attggacgct tttgcgcggc ggccgctatg ggaggcaggt ctcgactatg
     4561 gccacggcac cggtcacggg gtgggctcgt atctgtcggt gcatgaggga ccgcaaagca
     4621 tatcctcgcg cggcaccgtg caggcgttga aaccgggcat gatctgctcg aacgagcccg
     4681 gctattacaa gaccggcgaa tatggcatcc gcatcgaaaa cctggtggta gtgcgcgaac
     4741 ccgcgccaat cgcggacggt gaaaaaccca tgctgggttt cgagaccatc acgctggcgc
     4801 cgatcgatct ccattgcgtc gaaccggcaa tgctgacacc cgacgaaacg cgctggctca
     4861 atgcctatca cgcgcgcgtg cgcgaggctg tggcacctgc cttcgccggt gacgaaaaag
     4921 gacggctctg gctcgagcag gcgacacggg cgatctagtg cggttccaga acagcaaagc
     4981 gccgacggac cgcacggcta cgcaccgcct cgatcagaag ccgctgagcc atctccgcgg
     5041 gcggcctgcc gtcctgccac gcgatttgct tcaagctttg gaggacttcg tcgtccaaat
     5101 tgcggattac gatctgattc atgacactca ccaggttgcg tgatcccctg ccgtcttcaa
     5161 tctaacaggg gattgtgtcg ttttatcccg cgccgattaa ctggagccat tcgtcttctg
     5221 aaattatcgt tacattgaat tcctgcgcgt tcttcagctt cgaacccgcg ccgggtccgg
     5281 cgaccacgag atcggtcttc ttcgacaccg agccggcgac ttttgcgccc agcgattccg
     5341 cacgcgcctt ggcctcgttg cggctcatct tctcaagcgt gccggtgaag acgatggtct
     5401 tgcccgcgac cggagagtca gccgcagtgt gcggcgcaac atcggtcacc tcgatatgtg
     5461 gcatcagccg ggcgacggca tcgcgcgtat gccgatcgtc aaagaaattc ttgatcgcgc
     5521 cggccatgac atcgccgatc ccctcgatgg catcgaggtc cgtgactgcg gaatcgctct
     5581 ccatggagtc gagaaatgtc ttcgcggtgt ggaaattgcg ggccagaagt ttcgccgtcg
     5641 tctcacccac atggggaatg ctgagcgaca ggatgaaacg gtcgagagaa atcacgcgcc
     5701 gcgcttcgat ggctgcgagc agcttgttgc ccagaagata ttcggtcttc tgcgccttgg
     5761 ccttcttggc ggcggcctcc ttgccacgct tgaccgcatt gcgttcggag atcgcggcga
     5821 agtgccgggt caaaacggca tgaaacgcct ccggatggtt cttcaacgtg aacagatcag
     5881 ccggctcctt gaccagatcg gcctcgtgca gaagctggat gaattcgccg cccaagcctt
     5941 cgacatcgaa ggcggagcgc gaaacgaaat gatagagccg ctccaccacc tgcgcgggac
     6001 aggacagcgt gttgacgcag cgccgcgcgg attccggggc gccggtcttg ggattgatct
     6061 cccgcaccgc gtggctgccg cagaccgggc acttctccgg catatggtac ggcttcgtac
     6121 ccttcggccg gcgttccggc acaacgcgca ccaattgcgg gattacgtcg cccgcgcgct
     6181 gcaccacgac ggtatcgcca acgcgcacat ccttgcgcgc cacctcgtcc tcattgtgca
     6241 tggagacgtt ctgcaccacc acgccgccga cggtcaccgg cttgatgtgc gcaaccggcg
     6301 tcagcgcgcc tgtccggccg acatagacgg caatgcgctc gaccacgctc tcggcctgct
     6361 cggcgggaaa tttatgcgcg atggcccagc gcggcgaacg cgacacaaag cccagcctgt
     6421 tctgcagatc gagccggtcg accttgtaaa ccacgccatc gatatcgtag ccgaggtccg
     6481 cacgcgcgtg ttgaacctcc tcgtagaaag cgagcgcctc ctccacattg cggcagcggc
     6541 tcaccagcgg gttcaccgga aagccccaac ccttgaacgt ctgcagcacc tcccaatgcg
     6601 tcgcgccggg aagctccgat gcctcgcccc aatgataagc gaagaaacgc agcggccgcc
     6661 gcgccgtaat ctccggatcg agctgccgca aagatccggc tgcggcattg cgcggattgg
     6721 cgaaaatctt gtcgcccgct ttttcctgac gacggttcag ggcggcaaag tcggcgtggc
     6781 tcatatagac ctcgccgcgt acctcgaaaa tctccggcgg atgccggcca tggaatttct
     6841 tcggcacgtc ggagacggtg cggatattgg cggtgacgtt ctcgccttcc gcgccatccc
     6901 cacgggtggc gccgaggacg agctcgcctt tttcataccg cagactggac gacaatccgt
     6961 cgatctttgg ctcggccacc agcatcaatt cttggtcttc gggaagtgcc agaaagcgac
     7021 gcacgcgggc cacgaaatcg cgcacatcct catcggtgaa ggcgttgtcg agcgatagca
     7081 tgggacgcgc atggcgcacc ttttcgaatc gctccgcagg ttttgcaccc acgcgttggg
     7141 acgggctgtc gtgccggatc aattcgggaa agcgcttctc gatctcatcg ttgcgccggc
     7201 gcaatgcgtc atattcggca tcggtgatgg agggcgcgtc gtcctgatag taacgccggt
     7261 cgtgttcggc gatttcgtgc gcgagtcgcg ccagctctgt cttggcttcg ttttcggtga
     7321 gctgatccgg cgatttgctg ttggcggcgc tcacgcgcga actcgtttct tgggcttgga
     7381 ttgcgcctgg gattccgcaa ccagacggcg cgccgccgca cgtgcctcgt ccgacacggc
     7441 tgcgcccgaa agcatgcgtg cgatttcctc cacccgctcc tcgatgtcca gctcgctcac
     7501 gcgggtggca tccttctggc gcgcgatccg gaaatgccgt gtcgcgcggg cggccacttg
     7561 gggcgaatgg gtgacgagaa gcacctgcgt ggtttgggcg aggcgctgca atcgttcgcc
     7621 caccgcggcc gcaaccgcgc cgcccacgcc acggtccact tcgtcgaaca cgagcacggc
     7681 cggcgggctt accgccgcga gcgaaacctt cagtgcgagc gcaaaccgcg ccagttcgcc
     7741 gccggaggcg attttcgcga gaggtccaaa ggacgcgcct tcgatggtgg cgatctcgaa
     7801 cgcgatccgc tcgaatccgc tggctcctgc tgtgccttca tcaagcggcg cgaacgcgac
     7861 gcggaaccgc gcggcgccga gtttcaacgg agaaaattct gcggaaaccg cctcttccag
     7921 attcttggct gccgccgcgc gacacgccga aagttcgcgt gcgcgcgcgc ggtaatcggc
     7981 gcgcaggcgt tccacctcga cggtcgccgc ggtaatggcc gttccgctca cgtcaagaga
     8041 cgccagcttg gcctcgaatt catcacgcag tcggccaagc gaatccaccg gaacattgta
     8101 tttgcgggcc gccgctcgta gcgcgaacaa acggtcctcg acaatttcta atcttccggg
     8161 atcgttctcg agctgtctca gcagcacttc caacgtgctg cgcgcctcct ggaccagcgt
     8221 caacgccgaa tccagcgcgg tctccgccgc cgagacggca ctgcgccctt cggccggcag
     8281 ccgcgacaag cggcgaagcg ctgcggccaa ctgcacctcg gcgctggcgt cgcctccgac
     8341 gaagtccgca gctgcggaaa tttcctgcgc gaggcgtccc gcatttgtga gcagaccgcg
     8401 tgttgtagcc agcggctctt cctcgcccgg ctcgggcgct agatcgcgca gttcttttgc
     8461 cgcgtattcg agatattcga tctcctcagc cgcagccgcc tgcgcacggc gcaatgaggc
     8521 gagtcttccg gtcgttgcaa ccaatgcggc gtggcaacgg acgacctcct cggcaaggag
     8581 ggtgtgtccg ccgaacgcat ccagcagcag ccgatgcgtg gcgacatcga acagcccacg
     8641 atcatcggtc tgcccgtgga tttccagaag gagcgaacca atttcgcgcg cgagctgaat
     8701 gcccaccggg gaatcattca cataagcacg gctgcggccg tccgcggtta ccgcacgccg
     8761 cagcaggata tcattggctt cagcgtcaaa gccgttcgct ttcagcagca catgtgcggg
     8821 gtgatccggc gcgacatcga aaacagcgac gacagtggca gcttccgccc cggcgcggat
     8881 tgcggcgcgc cccacgccac gttcacccgc agctagacta agggcatcca gcagaatgga
     8941 tttgcctgca cccgtttcgc cggtcaggac attcaagccc ggcgcgaagt gaagctccgc
     9001 cttttcgatc agaacgatgt cgcgaacggt gagcgaggcc agcatgacct gcttt
//
LOCUS       DASYEG010000286         1915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1123711, whole genome shotgun sequence.
ACCESSION   DASYEG010000286 DASYEG010000000
VERSION     DASYEG010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123711"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VGM72_12250"
     CDS             <1..208
                     /locus_tag="VGM72_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uL13 family ribosomal protein"
                     /protein_id="HEY4266083.1"
                     /translation="FLSGKTPERVVEKAIQRMLPRGPLGKHQFAHLRVYAGPDHPHTA
                     QQPEVLDIVAMNPKNNNYRKAKAS"
     gene            309..704
                     /gene="rpsI"
                     /locus_tag="VGM72_12255"
     CDS             309..704
                     /gene="rpsI"
                     /locus_tag="VGM72_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY4266084.1"
                     /translation="MDRQGRAYATGKRKNAVARVWLKPGAGRIKINDKEENVYFARPV
                     LRMILRQPLVAAGRDGQYDVICTVVGGGLSGQAGALRHGISKALIAFEPGLRPVLKQG
                     GFLTRDPRVVERKKYGRPKARRSFQFSKR"
     gene            complement(770..1306)
                     /locus_tag="VGM72_12260"
     CDS             complement(770..1306)
                     /locus_tag="VGM72_12260"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4266085.1"
                     /translation="MEATDPTANSGHGAGSCVIRPAVETDLPAILAIYNEAVLNQTSI
                     WNDTIVDLADRQAWWETRVARGYPVLIAETGTEVTGYAAFGDFRPFHGYRHTVEHSIY
                     VAPHAWRRGIGRMLLEQLIAEARTRGKHVMIGGIGADNVASLALHARMGFSETARMPE
                     VGRKFGRWLDLVFVQKTL"
     gene            1404..>1915
                     /gene="argC"
                     /locus_tag="VGM72_12265"
     CDS             1404..>1915
                     /gene="argC"
                     /locus_tag="VGM72_12265"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEY4266086.1"
                     /translation="MPVKIFIDGAAGTTGLEIRERLQNRGEIELVVLGDKQRKDASAR
                     EQALNDSDIVILCLPDDAAREAVSLITNPNVRVIDPSTAHRVTEGWAFGFPELEAKRR
                     EELRHAMRVTNPGCYSTGFLSVMRPLVRNAIVPPDWPVLCNAVSGYSGGGRTMIAEFE
                     DSNAANYSQEV"
BASE COUNT          387 a          596 c          595 g          337 t
ORIGIN      
        1 attcctcagc ggcaagacgc ccgagcgcgt ggtcgagaaa gccatccagc gcatgctgcc
       61 gcgcggacct ttgggcaagc atcaattcgc gcacttacgc gtctatgccg gccccgatca
      121 tccgcatacg gcacagcagc cggaagtgct ggatatcgtt gcaatgaacc caaagaacaa
      181 caattaccgc aaagcgaagg cgagctgata tgtccgaggg agttcgcgat ttttccgatc
      241 tgaagtcgac actgatcaag gccaaggtgc ctgagcaggc gcaagccgcc aacgaaggcc
      301 gtccgaagct ggaccgtcag ggccgtgcct acgcgaccgg caagcgtaag aacgcggtcg
      361 ctcgcgtctg gcttaagccc ggggcgggcc gcatcaagat caacgacaag gaagagaatg
      421 tgtattttgc gcgccccgtg ctacgcatga tcctgcgcca accactggtc gcggcgggcc
      481 gcgatggaca atatgacgtg atctgcaccg tcgtgggcgg cggattgtcc ggtcaggcgg
      541 gagcgctgcg gcacggcatt tccaaggcgc tgatcgcctt cgaacccgga ttgcgaccgg
      601 tcctgaaaca gggcggtttc ctcacgcgcg atccgcgcgt cgtcgaacgc aagaaatatg
      661 ggcggcccaa agcgcgccgg agcttccagt tctccaagcg ctaaatacgg cacgcgccgc
      721 acatccttgt cagacagcac gccggagaaa tccggtgtgc tgtttgcgtt cagagggttt
      781 tctggacgaa aacgaggtcg agccaacggc caaatttgcg cccgacctcg ggcatgcgcg
      841 cggtctccga gaaacccatc cgcgcgtgca acgcgagcga cgcaacattg tccgcgccga
      901 ttcccccgat catgacatgc ttgccccgcg tgcgcgcctc ggcgatcaac tgctccagca
      961 gcatcctgcc tatcccgcgc cgccatgcgt gcggagcgac atagatggag tgctcgacag
     1021 tgtgccggta gccgtgaaag ggacgaaaat cgccgaaggc ggcatagccg gtcacttccg
     1081 tgccggtttc ggcaattaat acggggtaac cgcgcgccac gcgcgtctcc caccaggcct
     1141 gccggtccgc cagatcgaca atggtgtcgt tccagatgga ggtttggttc agcaccgcct
     1201 cgttgtagat cgccaggatc gccggaagat cggtctcgac ggccggccgg atcacacagc
     1261 tcccggcgcc gtgaccagaa tttgccgtcg ggtctgtcgc ctccatggac ctctcccgcc
     1321 gaatatgatg gaaagggccc ggcagccccg ctcgcatccg agcgtagccg gaaattctgt
     1381 caatgttcgc gtaaggacgt tctatgccgg tcaaaatctt catcgatggt gcggcgggga
     1441 ccacggggtt ggaaattcgc gagcggctgc aaaaccgcgg cgaaatcgaa ctcgtcgttc
     1501 tgggcgacaa acagcgtaag gatgcgtctg cccgcgaaca ggcgctcaac gattccgata
     1561 tcgtgatcct gtgccttccg gacgacgccg cgcgtgaagc ggtaagcctg ataacaaatc
     1621 cgaatgtccg ggtgatcgat ccgagcacgg cccatcgcgt gacggagggc tgggccttcg
     1681 ggtttcccga actggaagcc aagcgccgcg aggagctgcg gcacgccatg cgggtgacca
     1741 atcccggctg ctattcgacc gggtttctgt ccgtcatgcg gccgctggtg cgcaacgcaa
     1801 ttgtgccgcc cgattggccg gttctgtgca atgccgtctc cggctattcc ggcggcgggc
     1861 ggacgatgat cgccgaattc gaggattcca acgccgccaa ttacagccag gaagt
//
LOCUS       DASYEG010000287         4196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_5035907, whole genome shotgun sequence.
ACCESSION   DASYEG010000287 DASYEG010000000
VERSION     DASYEG010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5035907"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VGM72_12270"
     CDS             complement(<1..554)
                     /locus_tag="VGM72_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004813679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY4266087.1"
                     /translation="MQKTISVRGAREHNLKNVDVDIPRDKLVVITGLSGSGKSSLAFD
                     TVYAEGQRRYVESLSAYARQFLEMMQKPDVDHIDGLSPAISIEQRTTSKNPRSTVGTV
                     TEIYDYLRLLYARAGIPYSPTTGLPIESQTVSQMADRILALPEGTRLFVLAPIVRGRK
                     GEYRKELADLQKKGFQRVKVDGKI"
     gene            774..1235
                     /gene="ssb"
                     /locus_tag="VGM72_12275"
     CDS             774..1235
                     /gene="ssb"
                     /locus_tag="VGM72_12275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY4266088.1"
                     /translation="MAGSVNKVVLIGNLGRDPEVRRLTSGDPVVNLSIATSESWRDKA
                     SGERKEKTEWHRVVIFNENLAKVAEQYLKKGSKVYLEGQLQTRKYTDKDGAEKYSTEI
                     VLQKFRGELVMLDGRGEGGGDRAERPAMAGAGAEGGRSFQRGNDLDDEIPF"
     gene            1277..1936
                     /locus_tag="VGM72_12280"
     CDS             1277..1936
                     /locus_tag="VGM72_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY4266089.1"
                     /translation="MALIATLFLAGCAAMGSGVPSSGFANDAVKEAYLPLAQWNLDFK
                     GLETRHGAALVVAPHVAVTNAHNANLLPREAILAMSDYDLLFFRTEKSTRPSFAPASL
                     GEAVIAYGNGPGGRLRQAEGQMRFLDQQVLPLCAACRAQRVLGFEADAGPGFSGGPVV
                     DAKSGAVLGIIFGFRDSDGRRLMYAYDSATVLAEMHRLLDASAGVNDAWRACSNHRPK
                     C"
     gene            1997..2755
                     /locus_tag="VGM72_12285"
     CDS             1997..2755
                     /locus_tag="VGM72_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266090.1"
                     /translation="MAKTAKKDATPSSESRASIIATVTTPLGLLALIALIIEAMLAAF
                     AVRAQGQDFTILVYGMLASLLMFLAIVGGVLAFRPQALGPAAPSLLQVPAVPAKLKYD
                     FDVFLSAPMAAFTTEAAYTAGRADVLRVMAALKEHLDLERVYFAGETIESIAGFDAMA
                     VAVEEDLDKIRNSRIFVMLYPVKIVSSVLVEAGYALALRCPTLIFVRDLADLPFLLRN
                     AAEVLHYVRVVVEPDTPAILARLKKNGIKLIPAA"
     gene            2893..>4196
                     /locus_tag="VGM72_12290"
     CDS             2893..>4196
                     /locus_tag="VGM72_12290"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY4266091.1"
                     /translation="MSIEDDHKSADGGGPPGGNVSPVAIESELKRSYLDYAMSVIVSR
                     ALPDVRDGLKPVHRRILYSMHENGNDWNKPYRKSARIVGDVIGKYHPHGDQAVYDALV
                     RLAQDFSMRLPLIDGQGNFGSVDGDPPAAMRYTEVRRAKVAHWLTEDLEKGTVDFQDN
                     YDASEHEPIVMPARFPNLLANGAGGIAVGMATNIPPHNLGEVIDAALAYIEDPSISME
                     TLTEIVPGPDFPTGALILGRNAPRAALARGRGSILMRAKLQIEELRKDREAIIVTEIP
                     YQVNKARMLERIADLVRDKRVEGISELRDESDRHGMRVVIELKRDASAQVVLNQLYRF
                     SELQTSFGVNMLALNGGRPELMNLKQMIAAFVLFREEVVTRRTRHDLEKARDRGHTLV
                     GLAIAVANIDAVIALIRAAPDTATAKAQLMERAWPAKDVGPLV"
BASE COUNT          807 a         1219 c         1347 g          823 t
ORIGIN      
        1 tggattttgc cgtccacctt gacgcgctgg aagcccttct tctgcaggtc ggccagttcc
       61 ttgcggtatt cgcccttgcg gccgcgcacg atgggggcaa gtacgaaaag ccgcgtgccc
      121 tcgggcagcg ccaggatgcg gtccgccatc tggctgacgg tctggctttc gatgggcagg
      181 cccgtcgtgg gcgaataggg aatcccggcg cgcgcataga gcaggcgcag ataatcgtag
      241 atctcggtca ccgtcccgac ggtcgagcgc ggatttttgg acgtggtgcg ctgttcgatg
      301 gagatggcgg gcgacaggcc gtcgatatga tcgacatcgg gtttctgcat catctccagg
      361 aactggcgcg cataggccga caggctttcg acataacgcc gctggccctc ggcatagacc
      421 gtatcgaagg caagcgagga tttgccggag cccgacagac cggtgatgac cacaagtttg
      481 tcacgcggaa tatcgacgtc gacgtttttc agattgtgtt cgcgcgcgcc gcgaaccgag
      541 atggttttct gcattcaaga cccaacagct aaaacgccta cctatggcga ggcgcggcct
      601 gcctcgcaag cggcaagtcc ggtgcagcct acgctaggat tcgatactgg aacgaaaaga
      661 gaacatacgc taggattgcc gcccgagcaa atcgcgtgga caagtgtttg ccgcgggtag
      721 tgtgccgccg tatcgggcgc tagattgaaa tttcaggcaa aggagagatg ggtatggcgg
      781 gcagcgtcaa caaggtggtc ctgatcggca atttgggacg cgatcccgaa gtgaggcggc
      841 tgacttcggg cgatccggtc gtcaatctct cgatcgcgac ctcggaatcc tggcgcgaca
      901 aggcgagcgg cgagcgcaag gagaagaccg aatggcatcg cgtggtgatc ttcaatgaaa
      961 acctcgcgaa agtggcggag caatatttga agaagggctc caaggtctat ctcgaaggcc
     1021 agctccagac ccgcaaatac accgacaagg acggcgcgga aaaatattcg accgagatcg
     1081 tgctgcagaa attccgcggc gagttggtga tgctcgatgg gcgcggggaa ggcggcggcg
     1141 accgggcgga gcgtccggcc atggcgggcg ccggagccga aggcggacgc agcttccagc
     1201 gcggcaacga tctcgacgac gagattccgt tctgattgac ggcgttgcga ggcgcgacgg
     1261 gggggcgcgg gcgagcgtgg ctttgatcgc gacgctgttc ctggccggct gcgccgcgat
     1321 ggggtcaggg gtgccgtctt cgggattcgc gaatgacgcg gtcaaggagg catatctgcc
     1381 tctcgcgcaa tggaacctcg atttcaaggg attagagacc cggcatgggg cagccctcgt
     1441 tgttgccccc catgtggcgg tcacgaacgc acacaatgcc aatcttctgc cgcgcgaagc
     1501 gattttggcg atgtccgatt acgatttgct tttcttccgc acggaaaaat ctacgcggcc
     1561 ctccttcgcg ccggcttctt tgggagaggc cgtcattgcc tatggcaacg gccccggggg
     1621 caggctgcgg caggccgaag gacagatgcg attcctcgac caacaggtct tgccgctctg
     1681 cgcggcctgc cgggcgcagc gggttctcgg gttcgaagcc gatgccggcc ccggcttttc
     1741 gggtggcccg gtggtggatg ccaagagcgg cgctgtcttg gggattattt tcggctttcg
     1801 cgatagcgac ggccggcgat tgatgtatgc ctatgacagc gcgaccgtgc tggccgaaat
     1861 gcaccggctt ctcgatgcgt ccgcgggcgt gaacgatgcc tggcgggcct gttcgaatca
     1921 ccgcccgaaa tgctagttcc tgggcactgt atttccgtaa agaagcgcgg catttcttca
     1981 cggatcgagg gataacatgg cgaaaacagc aaagaaggat gccacgcctt cttccgaatc
     2041 ccgggcgagc attattgcga cagtgaccac gcccttaggc ctgctcgccc ttatcgctct
     2101 catcatcgaa gcgatgcttg cagcatttgc ggtgcgtgcg caggggcagg atttcaccat
     2161 cctcgtttat gggatgctgg cttcgttact gatgtttttg gcgattgttg gaggggtgct
     2221 ggcttttcgg ccccaggctc taggccctgc cgcaccgtca cttctgcaag tcccggcggt
     2281 tccggccaaa ctgaaatatg atttcgacgt gttcttgtcg gcgccaatgg ccgcctttac
     2341 caccgaagcg gcctatacgg cagggcgcgc cgacgtgctg agggtcatgg ccgcgctcaa
     2401 agagcatctg gatctcgaac gcgtctattt cgccggtgag acgatcgaat cgatcgcggg
     2461 ttttgatgcg atggcggtag ctgttgagga agatctcgac aagattcgca acagccggat
     2521 ttttgtcatg ctctaccccg tcaagatcgt gtcgagcgtt ttggtcgagg ccggatacgc
     2581 cttggccctg cggtgcccga cgctcatctt cgtgcgggac ttggcagacc ttccgttttt
     2641 gctgcgcaat gctgccgaag tgctgcatta tgtgcgggtg gtggtagaac ccgatacgcc
     2701 ggcgatcctc gcacgcctta agaagaatgg aatcaagctg attccggcgg catgaaaatt
     2761 tgccgcattt cggcaatcgt cacctttgag cacaagtcat gctcagggat attggctgcc
     2821 cggtgccata aatgctaatc cagtcgccaa cccgcgtaac agacgccagc gcgcgcttat
     2881 agagatgtaa gtttgagcat agaagacgac cataaaagcg ccgatggcgg cggtccgccc
     2941 ggcggcaatg tcagccccgt cgccatcgaa agcgagctga agcggtccta tctcgattat
     3001 gcgatgagcg tgatcgtgag ccgcgcgctc cccgatgtgc gcgatgggct taaacccgtg
     3061 caccggcgca tcctctattc gatgcacgag aacgggaacg attggaacaa gccgtatcgc
     3121 aagtccgccc gcatcgttgg cgacgtcatc ggcaagtacc acccgcatgg cgatcaggcg
     3181 gtgtatgacg cgctggtgcg cctcgcgcag gatttttcca tgcggctgcc gctcatcgac
     3241 gggcagggca atttcggctc cgtcgacggc gatcctccgg cggccatgcg ctataccgaa
     3301 gtgcgccggg cgaaggtggc ccattggctg accgaggacc tcgaaaaggg cacggtcgac
     3361 ttccaggaca attacgacgc cagcgagcac gaacccatcg tgatgccggc gcgctttccc
     3421 aacctgctgg ccaacggcgc gggcggcatc gcggtcggca tggcgaccaa tatcccgccg
     3481 cacaatctgg gcgaggtgat cgacgccgcg ctggcctata tcgaagatcc ctccatctcc
     3541 atggagacgc tgaccgagat cgtgccgggt cccgattttc ccaccggcgc gctgatcctt
     3601 ggccgcaacg cgccgcgcgc ggcgctggcg cgcgggcggg gctcgatatt gatgcgcgcc
     3661 aagctccaga tcgaggaact gcgcaaggac cgcgaagcga tcatcgtcac cgagattccc
     3721 tatcaggtga acaaggcgcg catgctggaa cgcatcgccg atctggtgcg cgacaaacgt
     3781 gtcgaaggca tttctgagct gcgcgacgaa agtgaccgcc acggcatgcg tgtggtgatc
     3841 gaactgaagc gcgacgcttc cgcccaggtg gtgctcaatc agctttatcg cttctcggaa
     3901 ttgcagacga gtttcggcgt gaacatgctg gcgctgaacg gcggccgtcc cgagctgatg
     3961 aatctgaagc agatgatcgc cgccttcgtg ctgtttcgcg aggaagtggt cacgcgccgc
     4021 acgcgccacg atctcgaaaa agctcgcgac cgcggccaca cgctggtggg tttggccatc
     4081 gcggtcgcca atatcgacgc ggtcatcgcg ctcattcgcg cggcccccga taccgcgacc
     4141 gccaaagcgc aattgatgga gcgcgcctgg ccggcaaagg atgtcggtcc gctggt
//
LOCUS       DASYEG010000288         2389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2865202, whole genome shotgun sequence.
ACCESSION   DASYEG010000288 DASYEG010000000
VERSION     DASYEG010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2865202"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VGM72_12295"
     CDS             <1..384
                     /locus_tag="VGM72_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY4266092.1"
                     /translation="QTMLGRIEEWRMWVLCDREPIADWSKGRVTLLGDAAHPMLQYLA
                     QGAGMALEDSLCLAKCLDEAESVEAALAAYSRARYLRTGRVQLTARLYGEFYHADGVR
                     RELRNAFVKNASYEALAWLYDPQPD"
     gene            complement(381..1934)
                     /locus_tag="VGM72_12300"
     CDS             complement(381..1934)
                     /locus_tag="VGM72_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEY4266093.1"
                     /translation="MPLNVTALIVFVFFFTLVTFVGFAAARWKRGDLDQLHEWGLAGG
                     KFGTLITWFLLGGDLYTAYTFIAVPALAFGAGALAFFAVPYTIVIYPFVFVVFPRLWS
                     VARARGYITAADFIRGRYGDRWLALAIALTGILATLPYIALQLIGLEVVIAAMGVQLA
                     GFLVYLPLILSFAILAAFDYFSGLRAPAAIAIIKDIMIYITVFAAVIVIPLEVGGWSK
                     VFAAVPPEKLLLPDNLGSYTGYATLFLGSAMALFLYPHSVTGVLSSSSRHVIRRNAAL
                     LPAYSFLLGLIALLGFMALAVGVREMPAYANAFKQFGPNFAVPALFLQVFPSWFVGFA
                     FASIGIGALVPAAIMSIASANLFSRNIYREFINPNCNPMQESRVAKTMSLVAKVGALM
                     FIIFLPHEYAIQMQLLGGIWIIHTFPAMIFGLYTRWLNSKALLIGWAVGMIVGTWMAA
                     ANKFGAIYEFQMFGIAIRAYIAITSVLLNFGVSVVLTLVFNMRTGSQRDETVPSDYYA
                     LEGIGGGGH"
     gene            complement(1956..2165)
                     /locus_tag="VGM72_12305"
     CDS             complement(1956..2165)
                     /locus_tag="VGM72_12305"
                     /inference="COORDINATES: protein motif:HMM:NF023183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HEY4266094.1"
                     /translation="MENREEGGNGGGRALLWLLLVPMIVLYVPLYNTIEPTLFGFPFF
                     YWFQLAWIFVSMIITAVVYYGTEPR"
     gene            complement(2202..>2389)
                     /locus_tag="VGM72_12310"
     CDS             complement(2202..>2389)
                     /locus_tag="VGM72_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266095.1"
                     /translation="VEFAELRVIFTHDGDVVKPGEQTHLLPRYFGPKATRNRSHPPES
                     ENFVLAEQLLGTMLNLA"
BASE COUNT          549 a          694 c          744 g          402 t
ORIGIN      
        1 caaaccatgc tcggccgcat cgaggaatgg cgcatgtggg tcttgtgcga ccgcgagccg
       61 attgccgatt ggagcaaggg gcgggtgaca ttgctcggcg atgccgccca tccgatgctg
      121 caatatctgg cgcaaggggc aggcatggcg cttgaggatt cgctatgcct cgcgaaatgt
      181 ctcgatgagg ccgagagcgt ggaggctgcc ttagccgcct attcgcgggc gcgctatttg
      241 cgtacgggac gcgtgcaatt gacggcgcgg ctctatggcg aattctatca cgccgacggc
      301 gtgcgccggg aattgcgcaa cgccttcgtc aaaaacgcca gctatgaagc gctcgcctgg
      361 ctgtacgacc cacaaccgga ttagtgcccg cctccgccga tgccttccag cgcataataa
      421 tctgaaggaa cggtttcatc gcgctggctc cccgtcctca tgttgaagac cagtgtcagc
      481 actacgctga caccgaaatt tagcagcacg gaggtgatcg cgatataggc ccggatggcg
      541 atgccgaaca tttgaaattc gtagatcgcg ccgaatttgt tcgctgccgc catccaggtg
      601 ccaacgatca tgcccacggc ccagccgatc agcagcgcct tggaattaag ccagcgcgta
      661 tagagaccga agatcatcgc ggggaaggtg tggatgatcc agatgccgcc cagcaattgc
      721 atctggatgg catattcgtg cggcaggaag atgatgaaca tcaaggcgcc gaccttggcc
      781 accagcgaca tggtcttggc cacgcgcgat tcctgcatcg ggttgcagtt cgggttgatg
      841 aattcgcgat agatgttgcg gctgaagaga ttggccgagg cgatggacat gatcgcggcc
      901 ggcaccaacg cgccgatgcc gatggaggcg aaggcaaagc ccacgaacca ggatgggaag
      961 acctgaagga acagcgcggg gaccgcgaaa ttggggccaa attgcttgaa ggcgttggca
     1021 taggcgggca tctcccgcac gccgaccgcc agcgccatga agcccagaag cgcgatgaga
     1081 ccgagcagga acgaataggc cggaaggagc gctgcgttgc ggcggatcac atggcggctg
     1141 gaggaggaga gcacgccggt caccgaatga ggatagagga acagcgccat ggccgagccc
     1201 aggaacaacg tcgcgtagcc ggtataggag ccgagattgt cgggcagcag cagcttttcc
     1261 ggcggcaccg ccgcgaagac cttgctccag ccgccgactt ccagagggat cacgatcacg
     1321 gccgcgaaca ccgtgatgta gatcatgatg tccttgatga tcgcgatggc cgcgggcgcg
     1381 cgcaagccgc tgaaatagtc gaacgccgcc aggatcgcga aggagaggat gagcggcaga
     1441 tagacgagaa agcccgccaa ttgaacgccc atggccgcga tgacgacctc aagcccgatc
     1501 aactgcagcg caatgtaggg cagcgtcgcc aggatgccgg tcagtgcgat ggcgagcgcg
     1561 agccagcggt cgccgtatcg gccgcggatg aaatcggctg ccgtgatgta gccgcgcgcg
     1621 cgcgccaccg accacagccg tgggaacacc acgaagacga aaggatagat cacgatcgtg
     1681 tagggcaccg cgaaaaatgc cagcgcgccc gcgccgaacg ccaaggccgg caccgcgatg
     1741 aacgtatagg ccgtgtacag atcaccgccc aaaaggaacc aggtgatgag ggttccgaac
     1801 ttgccgccgg ccaaccccca ttcgtgcaat tgatcgagat cgccccgttt ccagcgcgcc
     1861 gcggcgaagc ccacgaacgt caccagcgtg aagaaaaaca caaagacgat cagagccgtc
     1921 acattcaatg gcattacgcg tttccccccg gccgcttagc gcggttccgt gccgtaatag
     1981 acgacggcgg taatgatcat gctgacgaaa atccaggcga gctggaacca atagaagaat
     2041 ggaaatccga acagcgtagg ctcgattgtg ttgtacaagg gcacatagag aacgatcatc
     2101 gggaccagca gcagccagag cagggcgcgc ccgccgccgt ttccgccttc ttcgcgattt
     2161 tccatcttgc tccccccggg ctcttctccc ctgacctctg attaagccaa gtttagcatt
     2221 gtgccaagca gttgctccgc caacacaaaa ttttcgctct ctggcgggtg gctgcggttt
     2281 cgggtagctt tgggcccaaa ataacgaggc aggaggtgcg tttgttcgcc aggcttaacc
     2341 acgtcgccat cgtgagtgaa aattacgcgc agctcagcaa attctacga
//
LOCUS       DASYEG010000289         5416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4891487, whole genome shotgun sequence.
ACCESSION   DASYEG010000289 DASYEG010000000
VERSION     DASYEG010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5416
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4891487"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VGM72_12315"
     CDS             complement(<1..384)
                     /locus_tag="VGM72_12315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4266096.1"
                     /translation="MNNSPQVKLAARGVRLSFANERMKRQLPVLGGIDLEVREGELVA
                     IVGPSGCGKSSFLNAVDGLVKLTGGDIRVDGKTIVSPGPDRAMVFQQDSLFPWRTVLG
                     NVTYGLELQKRLPKEEMRARAAALVD"
     gene            complement(509..1519)
                     /locus_tag="VGM72_12320"
     CDS             complement(509..1519)
                     /locus_tag="VGM72_12320"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4266097.1"
                     /translation="MQVFRFARLAVAAILAVPMAHGTPGFAQGAARKLIPLRVGYDGY
                     SMTTAPMNYAVEKGIFKKYGLDVTLAYIDGGTTLSAAMVGGSMGIAQNGYAPAAAAAV
                     AGADVVFIGGISNQLPFQLVVKGNIKSAADLKGKKIAISRYGATSDVAATYTLRSLGL
                     KRTDVTILQLGGEGTRTAAMLSGQIDASLEQYPRTGELEEKGFHVLADCLALASDYPN
                     TSYVSTRAFIAKNPDIVKHFLMGISDGIHSFKTNRADALKSAAAFLKAKPGPALDKAY
                     DIFTTKVYPDVPRPSMKGIALVLDELKTRVPAAAKVKPEQLVYTQPLDVLEKEGFFAG
                     LK"
     gene            complement(1557..2396)
                     /locus_tag="VGM72_12325"
     CDS             complement(1557..2396)
                     /locus_tag="VGM72_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266098.1"
                     /translation="MSGFVSRNSRLIASLAVCATALIPVLTRAAPTAAAPPDFSSSSE
                     TAWVGIGGGNFLAVPGSPAPVGQDPHYHYQPNGPGQASFRIGDVTNPNLKQWAKDVMQ
                     KDNDEVLKGKIAYSPRSSCKPGGVPGFIAMGGGALFFVQSPSEVTMIFDGNAETRHIY
                     LNVPHTAHPKPSWYGDSVGHYEGDTLVVDTIALNTKTFVDAYRTPHTEKLHVTERWRL
                     IDNGDKLEVVTTVNDPDTFNQPWQAMRRYQRGKRTLVENICAENNSNLFDYGTPTAGK
                     PDF"
     gene            complement(2461..3270)
                     /locus_tag="VGM72_12330"
     CDS             complement(2461..3270)
                     /locus_tag="VGM72_12330"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4266099.1"
                     /translation="MSVKNTGVIGLGAMGYQMARHMVNKGFAVAGYDVAPVAAQRAMD
                     AGVTIVDSPAEAGAGADVVIVMVATDAQVEDVVLHSGLLDRLPKGAVICVASSTSPET
                     ARELASVCAKRGLGLLDTPVVLGQEAADNGTLTVFCGGEVAHFEKARPALSAFGANVM
                     HVGPSGTGQLTKTANNMLLWACMVANYEVLSFAKKLGADVPKLIAALQHSSGANWSLE
                     RWGKSTGKWAEKDMDVALELAQDAKMPVPLAALVDQLMKGMNQEKMKALLA"
     gene            complement(3267..5006)
                     /locus_tag="VGM72_12335"
     CDS             complement(3267..5006)
                     /locus_tag="VGM72_12335"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY4266100.1"
                     /translation="MAELGAVDLEIIKASLSGIVQEMQNSLFRTGFSTIVRESQDASC
                     AILDTNADVIAQHVVLPLHMGAFPACCAAVIRGYGGDIVEGDAYLINHPYEGGSPHAP
                     DMAVITPIFFARELIGFCGSIAHKSDIGGPVPGSCSGQARETFNEGLHLPAVRYVRGG
                     HRNVEIERIIAANSRTPELVLGDIRGQLGASRLGEQRMDELIAKFGKDTALASFQRLL
                     DLAQAKMKAAMVEWTDGRFEAERFVDDDGIDLNKPVRVHVVVEKKGDAIRFDFSGSAD
                     QTKGPANVRPPLVKAAVGYVLISLVDPLIFINSGIMRAFEVTTREGSVLNPRFPAAVN
                     TYNATVHAMVEALFAALSHAVPARARADGCGSRSIIIGGRATSAGKTYVQYEIVGGGA
                     GGRATKDGASGTSVNQSNAKIASIEIIESEFPTRVQRFELIPDSGGAGKFRGGLGILR
                     EYVNLADARFSIRSSKHVIPPLGAREGGNGRTGDLTINPGTADEKHLPTRYADYPLKA
                     GDIFRLETPGGGGLGDPHQREPQSVLADVAQGYVSQERAARDYGVAVVKKDSAWRIDE
                     QATAKLRLGKGEG"
     gene            complement(5008..>5416)
                     /locus_tag="VGM72_12340"
     CDS             complement(5008..>5416)
                     /locus_tag="VGM72_12340"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266101.1"
                     /translation="ITIACDYPLGDGSIASLRARFDETHRQMFGHTAPEEPVEIVSYR
                     LRGVGQVPPVALPKHRSEGRALGDALRETRKARFGGVTLDCPVYQRERLDVGIAFAGP
                     AIVDQLDATTVIPPGMRARVDEYKNILIGGGAV"
BASE COUNT         1000 a         1732 c         1640 g         1044 t
ORIGIN      
        1 atcgaccaac gctgccgccc gcgcgcgcat ctcctctttc ggcaggcgct tctgcagttc
       61 caggccgtac gtcacattgc cgagcaccgt gcgccaggga aaaagcgaat cctgctggaa
      121 caccatggcg cgatccggac ctggcgaaac gattgtcttg ccatcgacgc ggatgtcgcc
      181 gccggtcaat ttcaccagcc catcgaccgc gttcaggaag ctggatttgc cgcagccgga
      241 aggcccgaca atggcgacaa gttcgccttc gcgcacctcc agatcgatgc cgccgagtac
      301 gggaagctgc cgtttcatcc gctcattggc gaagctgagc cgcacgcctc ttgctgcgag
      361 cttcacctgc ggcgaattgt tcatggaatt ttccatcccg tcatggccgg ccatccacgg
      421 acactaccgc aaacggtgtt catggatcgc cgcgacaagc gcggcgatga cgaaatattt
      481 attgcttcag aacagcccgc gacaaacttc atttcaaccc ggcgaagaag ccttcctttt
      541 cgaggacatc gaggggttgg gtgtaaacga gctgctcagg tttcaccttc gccgccgccg
      601 gcacgcgcgt cttcaattcg tcgagcacca gcgcgatgcc tttcatcgag ggccgcggca
      661 catcggggta aaccttggtg gtgaaaatgt cataggcctt gtccagcgcc ggtcccggtt
      721 ttgccttcag gaaggcggcg gccgatttca gggcgtccgc ccgattggtc ttgaaactgt
      781 ggatgccgtc ggaaatcccc atcaggaagt gcttcacgat gtcgggattc ttggcgatga
      841 aagcgcgcgt cgaaacataa gaggtgttgg gataatcgct cgcgagcgcg agacaatcgg
      901 cgagcacgtg gaagcccttt tcctccagct ccccggtgcg tggatattgc tccagcgagg
      961 cgtcgatctg gcccgacagc atggctgccg tgcgtgtgcc ctcgccgccc aattgcagga
     1021 tggtcacatc ggtgcgcttg agacccaggc tgcgcaacgt gtaggtcgcg gccacatccg
     1081 atgtcgcgcc ataacgcgat atcgcgattt tcttgccctt cagatcggcg gccgatttga
     1141 tattgccctt cacgacaagc tggaacggca attgattgga aatgccgccg atgaagacga
     1201 catcggcacc ggccacggcc gccgccgccg ccggcgcata gccgttctgc gcgatcccca
     1261 tcgagccgcc caccatggcg gccgaaagcg tcgtgccgcc atcgatatag gcgagcgtca
     1321 cgtcgagccc gtatttcttg aagatgccct tttcgaccgc gtaattcatc ggcgcggtgg
     1381 tcatggaata gccgtcatag ccgacgcgca agggaatcag tttccgggcg gccccctgcg
     1441 cgaaaccggg tgtcccatgc gccatcggca cggcgaggat tgccgcaacc gccaaacgcg
     1501 caaaccggaa aacctgcact ccgttcctcc tgatgttcaa gaatcgggct tgatgttcaa
     1561 aaatcgggtt ttccggcggt cggggtgccg taatcgaaca ggttggaatt gttctcggcg
     1621 cagatgttct ccaccagcgt gcgcttgccc cgctgatagc ggcgcatcgc ctgccagggc
     1681 tggttgaacg tatcgggatc gttgaccgtg gtgacgactt cgagtttgtc gccattgtcg
     1741 atcagccgcc agcgctcggt gacgtgcagt ttttcggtat gcggcgtgcg ataggcatcg
     1801 acaaacgtct tcgtgttcag cgcgatggtg tcgaccacca gcgtatcgcc ttcataatgg
     1861 ccgaccgaat ctccgtacca ggacggttta ggatgagcgg tatggggcac gttcagataa
     1921 atgtgccggg tctccgcatt gccgtcgaag atcatcgtca cttcgctcgg cgattggacg
     1981 aagaacaaag cgccgccccc catcgcgatg aagcccggaa cgccgcccgg cttgcaggat
     2041 gagcgcggcg aataggcgat cttgcctttg agaacctcgt cattgtcctt ttgcatgaca
     2101 tccttcgccc attgcttgag attggggttc gtgacgtcgc cgatacgaaa gctcgcctgc
     2161 ccggggccgt tgggctgata atgataatgc gggtcctggc cgacgggcgc gggcgagccg
     2221 gggacggcaa gaaaattccc gccgccgatg ccgacccatg ccgtctcgct ggacgaggaa
     2281 aaatcgggcg gcgccgccgc ggtgggtgct gcgcgggtga ggaccggaat cagcgcggtc
     2341 gcgcagaccg cgagcgaggc gatgagccgg gaatttcttg aaacaaatcc ggacatcgtt
     2401 tctctccctg gtccgcgctg ttcctgccct tgccgttcta tactcgtatc gccggcacgc
     2461 tcacgccagc agcgccttca tcttctcctg attcatgccc ttcatgagtt gatcgacaag
     2521 ggcggccagc ggcaccggca ttttcgcatc ctgagccaat tccaacgcca catccatgtc
     2581 cttctccgcc catttgccgg tgcttttgcc ccagcgttcg agcgaccaat tcgcgccgga
     2641 cgaatgttgg agggccgcga tcagcttggg cacatccgcg cccagtttct tggcgaaaga
     2701 cagcacctcg taattcgcca ccatgcaggc ccacagcagc atgttgttgg cggtcttggt
     2761 gagctggccg gtgccggatg ggccgacatg catcacattg gcgccaaagg ccgagagcgc
     2821 gggtctcgcc ttttcgaaat gggctacctc gccgccacaa aaaaccgtga gcgtgccatt
     2881 gtcggcggct tcctggccca gcaccacggg cgtgtcgagc aggccgagcc cgcgtttggc
     2941 gcagacgctt gccagttcgc gcgcggtttc gggcgaggtc gaactggcga cacagatgac
     3001 cgcgcctttg ggcagccggt cgagcagtcc cgaatgcagc accacgtctt cgacctgcgc
     3061 gtcggttgcg accatcacga tcaccacgtc cgcgcccgcg ccggcttcgg cggggctgtc
     3121 gacgatggtg acgcccgcat ccatggcgcg ctgtgcggca accggggcca catcatagcc
     3181 ggcgacagcg aaacccttgt tcaccatgtg ccgcgccatc tgatagccca tggcgcccaa
     3241 gccaatcacg ccggtgtttt tcacgctcat ccttcgccct tccctaaacg caatttcgcg
     3301 gtcgcctgct cgtcaatccg ccaggcgctg tcctttttca ccaccgccac gccgtaatcg
     3361 cgcgcggcac gctcttgtga aacataaccc tgcgccacat cggcgagcac gctttgcggc
     3421 tcgcgctgat gcggatcgcc cagcccaccg ccgcccggcg tctccagccg gaagatatcg
     3481 cccgccttca gcggataatc ggcatagcgc gtgggcagat gcttttcatc ggcggttccg
     3541 ggattgatgg tgagatcgcc ggtgcggcca ttgccgccct cgcgcgcgcc cagaggcggg
     3601 atcacatgtt tggacgagcg gatggaaaat cgcgcatcgg caagattgac atattcgcgc
     3661 aggatcccca gaccgccacg gaattttccc gcgccgccgg aatcgggaat cagctcgaaa
     3721 cgttgcacgc gcgtgggaaa ttcgctttcg atgatttcga tggaggcgat cttcgcattg
     3781 ctctgattga ccgacgtgcc cgaggcgccg tccttggtcg cacgtccgcc cgcaccaccg
     3841 cccacgattt catattgcac ataggtcttg cctgccgagg tcgcgcggcc gccgatgatg
     3901 atggagcggc tgccgcagcc atcggcccgc gcgcgcgccg gaaccgcatg cgagagcgcg
     3961 gcaaacaacg cctccaccat cgcgtgcacg gtcgcattgt aggtgttgac ggcggcgggg
     4021 aaacgcgggt tcagcacgct gccctcgcgc gttgtcacct cgaaggcgcg catgatgccg
     4081 gaattgatga agatgagcgg atcgaccagc gaaatgagca catagccgac cgcggccttg
     4141 accagaggcg gccgcacatt ggccgggccc ttggtctgat cggccgaacc cgagaagtcg
     4201 aagcggatcg catcgccctt tttttccacc acgacatgga cgcgcacggg cttgttcaga
     4261 tcgatgccgt catcgtcgac gaaacgctcc gcctcgaagc gcccatccgt ccattcgacc
     4321 atggcggctt tcattttcgc ttgcgccaaa tcgagcagcc gctggaaact cgcaagcgcg
     4381 gtgtccttgc cgaatttcgc gatcagctcg tccatgcgct gctcgccgag acggctcgcg
     4441 ccgagctgcc cgcgtatgtc gcccagcacc agctcaggcg tgcggctgtt ggcggcgatg
     4501 atgcgctcga tctcgacatt gcggtggccg ccgcgaacat agcgcaccgc cggcagatgc
     4561 agcccttcgt tgaaggtctc gcgcgcctgg ccggagcatg accccggcac cggaccgccg
     4621 atatcgctct tgtgcgcaat cgagccgcag aatccgatca gctcgcgcgc gaaaaagatg
     4681 ggcgtgatca ccgccatgtc gggagcgtgc gggctgccgc cttcataggg gtggttgatg
     4741 agataggcat cgccttcgac gatgtcaccg ccatagccgc ggatgacggc ggcacaacag
     4801 gccggaaacg cgcccatgtg cagcggcagc acgacatgct gcgcgatcac atcggcattg
     4861 gtgtcgagaa tggcgcagga ggcatcctgc gattcgcgca cgatggtcga gaagccggtg
     4921 cggaagagcg aattctgcat ctcctgcacg atgcccgaaa gactcgcttt gatgatttcg
     4981 agatcgactg cgccaagctc agccatctca gaccgccccg ccgccgatga ggatgttttt
     5041 gtattcatcg acgcgcgcgc gcattcccgg cggtatcacc gtggtggcat cgagctgatc
     5101 gacgatggcc ggccccgcga aagcaatgcc aacatccagc cgttcgcgct gatagacagg
     5161 gcaatccagt gtgacgccgc caaagcgcgc tttgcgggtt tcgcgcaagg catcgccaag
     5221 cgcgcggcct tcggatctgt gcttgggcag cgcgaccggc ggcacctggc ccacaccacg
     5281 cagacgatag gagacgattt cgaccggctc ctcgggcgcg gtgtgaccga acatctgtct
     5341 gtgcgtttca tcgaagcgcg cgcgtagact tgcgatgctg ccgtcaccaa gcggataatc
     5401 gcacgcaatc gtgatc
//
LOCUS       DASYEG010000290         2118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4024394, whole genome shotgun sequence.
ACCESSION   DASYEG010000290 DASYEG010000000
VERSION     DASYEG010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4024394"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2102)
                     /gene="gyrA"
                     /locus_tag="VGM72_12345"
     CDS             complement(<1..2102)
                     /gene="gyrA"
                     /locus_tag="VGM72_12345"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY4266102.1"
                     /translation="MSDTSAATPPSGGSVHLPIAIEDELKRSYLDYAMSVIVSRALPD
                     ARDGLKPVHRRILFSMHENNRDWNKPYRKCALIVGDVMGKYHPHGDQSIYDALVRMAQ
                     DFSMRAPLIDGQGNFGSVDGDPPAAMRYTESRRSKIAHMLSEDIDKDTVPFQDNYDGS
                     EREPIVLPARFPNLLVNGAGGIAVGMATNIPPHNLGEVIDACLAYIDDPSIGMDALTE
                     IVPGPDFPTGALLIGKQAARGALARGRGSILMRARCAIEEIRKDRDAIIVTELPYQVN
                     KARLQEKIAELVRDKRIEGVGDIRDESDRHGMRVVIEIKRDASAEVVLNQLYRFSELQ
                     TTFGVNMVALNAGRPMIMNLKDMIGAFTNFREEVVMRRTRFELNKARDRGHILVGLAI
                     AVANIDDVIHLIRNAPDTATAREQLMERAWPAKDVAPLVLLIADPRHIMLEDGTIKLS
                     EEQAKAILEIRLARLTALGRDEIGDEVTKLSEFIKDYLDILRSRPRVLTIVKDELKAI
                     KDEFATPRRTELVDADVEIEDEALIEREDVAITVTHAGYIKRTPIAEYRVQGRGGKGR
                     AGMSTREEDFATNIFVANTHAPMVFFSSTGMAYKLKVWRLPEARIQGKGKAMVNLLPL
                     SEHERITTILPLPEDESAWEKLNVVFATKSGDVRRNELSDFVNINKAGKIAMKLEAGD
                     GIVDVQICTEQDDVLLTTR"
BASE COUNT          394 a          627 c          658 g          439 t
ORIGIN      
        1 cgcgtggtga gcagcacatc gtcctgctcg gtgcagatct gcacgtccac gatgccatcg
       61 cccgcttcga gcttcatggc gatcttgccg gctttgttga tgttcacgaa gtcggagagt
      121 tcgttgcgcc gcacgtcgcc ggacttggtg gcgaacacga cattcagctt ctcccacgca
      181 ctttcatctt cgggcagcgg aagaatggtg gtgatgcgct catgctcaga caatggcagc
      241 aaattcacca tcgccttgcc tttgccctga atgcgggctt cgggaaggcg ccagaccttg
      301 agcttgtagg ccataccggt ggaggagaag aacaccatcg gcgcatgggt gttggcgaca
      361 aagatgttgg tggcgaaatc ttcctcgcgc gtcgacatgc cggcgcggcc tttgccgccg
      421 cggccctgca cgcggtattc ggcgatgggt gtgcgcttga tataaccggc gtgggtgacg
      481 gtgatcgcga catcctcacg ctcgatcaaa gcctcgtctt cgatctccac atcggcatcg
      541 accagttcgg ttcttcgcgg cgtggcgaac tcgtctttga tcgccttcag ttcgtctttc
      601 acgatggtca gaacacgcgg gcgcgagcgc aggatgtcga gataatcctt gatgaattcg
      661 ctgagcttgg tgacttcatc gccgatttcg tcgcggccca gagcggtcaa ccgcgcaagg
      721 cggatttcga ggatggcctt agcctgctct tccgagagct tgatcgtgcc gtcttccagc
      781 atgatatggc gcggatcggc gatcagcagc accagcggcg ccacatcctt tgccggccat
      841 gcgcgttcca tcaactgctc gcgggccgtc gcggtgtcgg gcgcgttgcg gatgagatgg
      901 atcacatcgt caatattggc gacagcaatg gcgaggccga ccaagatgtg cccccggtcg
      961 cgcgccttgt taagctcgaa ccgcgtgcgg cgcatcacaa cttcctcgcg gaagttggtg
     1021 aaggcgccga tcatgtcctt caggttcatg atcatgggac ggccggcatt cagcgccacc
     1081 atgttcacgc cgaaggtcgt ctgcagttcg gagaaacgat agagctggtt cagcaccacc
     1141 tccgccgacg cgtcgcgctt gatctcaatg accacgcgca tgccatggcg atcgctttcg
     1201 tcgcggatgt cgccgacgcc ttcgatgcgc ttgtcgcgca cgagttcagc gatcttctcc
     1261 tgcaaacgcg ccttgttcac ctgataagga agctcggtga caatgatggc gtcgcggtct
     1321 ttgcggattt cctcgatggc gcagcgtgcg cgcatgagga tcgaaccgcg gccgcgtgca
     1381 agcgcgccac gcgcggcttg cttgccaatg aggagcgcgc cagtcgggaa gtccggcccc
     1441 ggcacgattt cggtcagcgc atccatgccg atggacggat cgtcgatata ggcaaggcag
     1501 gcgtcgatca cttcgcccag attgtgcggc gggatgttcg ttgccatgcc gacggcaatg
     1561 ccgcccgcgc cgttgacgag caaattggga aagcgggcgg gaagaacaat cggctcgcgc
     1621 tcggagccgt cataattgtc ctggaacggc accgtgtcct tgtcgatgtc ctcggacagc
     1681 atgtgcgcaa tcttggaacg acgcgattcc gtgtaacgca tcgccgcggg cggatcgccg
     1741 tcgacagaac cgaaattgcc ctgcccgtcg atgagcggcg cgcgcatgga aaaatcctgc
     1801 gccatacgca ccaaggcgtc atagatcgat tggtcgccat gcggatgata cttacccatc
     1861 acgtcgccga cgatcagcgc gcatttgcga taaggcttgt tccagtcgcg attgttctca
     1921 tgcatcgaaa acaggatgcg ccgatgcacc ggcttcaggc cgtcacgcgc gtccggcagg
     1981 gcgcgcgaaa cgatgacgct catggcgtaa tcgaggtagg aacgcttcag ttcgtcctcg
     2041 atggcgatgg gcaaatgcac agagccgccg gaggggggag tggcggcgga ggtgtcgctc
     2101 aaaatacgtt ccttttca
//
LOCUS       DASYEG010000291         2388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2144704, whole genome shotgun sequence.
ACCESSION   DASYEG010000291 DASYEG010000000
VERSION     DASYEG010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2144704"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="VGM72_12350"
     CDS             <1..1206
                     /locus_tag="VGM72_12350"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4266103.1"
                     /translation="RTLMGAAAATAVMPTFRARAQAKPVIKIGVMNDQSGPYRDVNGP
                     TGVACTKQAVQEFAAHGFDVEVVDADHQNKPDVAVSLAREWFDQRGVDIIMDIAATSC
                     ALALASIVKEKNKIMIATSTASSDITGKACTPNSMHWSFDTYMEAKSTGGAMVKAGGD
                     TWYFITPNYAFGAATQRDTSHFVEAGGGKVLGSQVYPFPETTDYSAFLIQAQASGAKI
                     LGICGAGADLINIIKQAHEFGLSKTMKPAAMVAYTTDMHSVGIEMAAGTRLSETYYWD
                     LNERTRSFQKRIQPKVKLWPNMAQAGNYSCTLHYLKACHDMGAAQAKLSGAETTARMK
                     AMPTDDDCFGPGKIREDGRKITPAYLFEVKQPHESKHEWDLYKLIGITPAEDAFRPLS
                     DHACPLVKA"
     gene            complement(1246..2031)
                     /locus_tag="VGM72_12355"
     CDS             complement(1246..2031)
                     /locus_tag="VGM72_12355"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEY4266104.1"
                     /translation="MRSTLLLSCTMFAAHAEAARHSRPVKAHTIASHHTVASHHAHAL
                     STRMAFSSHARFSHAMHLVGRRYAMRYHGISCVPYAREVSGIEVAGNAWQWWDNAEGR
                     YARGDRPEAGSVLNFRANGRMRLGHVAVVSSVVNPREIMVDHANWPGAGGRGGISRDV
                     AVVDVSEANDWSAVRVELGRGGEFGSIYPTYGFIYDRPDSGRVTADISRPAPIEAINR
                     APSDLRPAAERPWHTVEEVAEAPDTSARKIDLTVPAASLRFGK"
BASE COUNT          483 a          703 c          795 g          407 t
ORIGIN      
        1 cgcactctga tgggtgccgc cgcggccacg gccgtaatgc cgacattccg agctcgggcg
       61 caggcgaagc cggtgatcaa gatcggggtg atgaacgacc agtccggccc gtatcgcgac
      121 gtgaacggcc cgaccggggt cgcctgcacc aagcaggcgg tgcaggagtt cgccgcccac
      181 ggtttcgacg tcgaggtcgt ggacgccgac catcagaaca aaccggatgt cgcggtgtcg
      241 ctggcgcgcg agtggttcga tcagcgtggt gtcgacatca tcatggatat tgctgcgacg
      301 tcgtgcgcgc tggcgctggc gtcgatcgtc aaagagaaaa acaagatcat gatcgccacc
      361 agcacggcgt cgtcggacat caccggcaag gcatgcacgc cgaattccat gcactggtcg
      421 ttcgacactt atatggaggc gaaatccacc ggcggtgcga tggtcaaagc cggcggcgat
      481 acctggtact tcatcacccc gaactatgcg ttcggcgccg ccacccagcg cgacaccagc
      541 catttcgtcg aggccggcgg cggcaaggtg ttgggatcac aggtgtaccc attcccggaa
      601 accacggatt attcagcatt cctgatccag gcacaggcgt ccggcgcgaa gatcctcggc
      661 atctgcggcg caggcgccga cctgatcaac atcatcaagc aggcgcacga attcggcctg
      721 tcgaagacga tgaaaccggc agcgatggtc gcctacacca ccgacatgca ctcggtgggg
      781 atcgagatgg cggccggcac gcggctgtcg gagacgtatt actgggacct gaacgagcgg
      841 acgcggagct tccagaagcg catccagccg aaggtgaagc tgtggccgaa catggcgcag
      901 gcgggcaact attcctgcac gctgcactac ctgaaggcgt gccatgacat gggggcggca
      961 caggcgaaac tgagcggagc ggagacaacg gccaggatga aggcgatgcc gaccgacgac
     1021 gactgcttcg ggccgggcaa gatccgcgag gacgggcgca agatcacccc ggcctatctg
     1081 ttcgaggtca agcagccgca cgagagcaag catgagtggg atttgtacaa gctgatcggc
     1141 atcacgccgg cggaagacgc cttccggccg ctgtcagatc atgcttgtcc gctggtgaag
     1201 gcgtagacgc cgggtgggcc tgtcgctgcc gggcagcggc gggcgttatt tcccgaagcg
     1261 aagggaagcc gcgggcaccg tcaggtcgat tttgcgggcc gaggtgtccg gcgcctcggc
     1321 gacttcttcg acggtgtgcc agggacgttc tgccgcgggg cgaaggtcgg acggcgcccg
     1381 gttgatggcc tcgatcgggg cggggcggct gatgtcggcg gtcacccggc cgctatcggg
     1441 gcgatcgtag atgaagccgt aggtcgggta gattgatccg aactctccac cgcggcccaa
     1501 ttcgacacgc accgcggacc agtcgttggc ctcggacaca tcgaccaccg cgacatcgcg
     1561 ggagatgccg ccacggccgc cggctccggg ccagttggca tgatccacca tgatttcacg
     1621 cgggttcacc acgctcgaaa caacggcgac gtgacctagg cgcatgcggc cattcgcacg
     1681 gaaattgaga acgctgccgg cttcgggccg atcaccgcgg gcgtagcggc cctcggcgtt
     1741 gtcccaccat tgccaggcat tgccggcgac ctcgatcccg gagacctcgc gggcgtacgg
     1801 cacgcaggaa atgccgtgat agcgcatggc gtatcgacgg ccgaccaaat gcatcgcatg
     1861 ggagaaccgg gcgtgcgacg agaaagccat gcgggtgctg agcgcgtgcg catgatgcga
     1921 ggcgacggta tgatgggatg cgatggtatg cgctttgacg gggcggctat ggcgtgcggc
     1981 ttcggcgtgg gcggcaaaca tggtgcaact gagcaggagc gtcgagcgca aaagccaagc
     2041 tttagttcct gttccgaccg accgcataga ctcgtctccc gcacaaacgc tagtcaagac
     2101 gtgcggtaac agcagggttg caaaggtgac aaccctatcg gggcaagatt cctcccgcgt
     2161 taactcattt cgccattttt ggcctgtgga taaccatagt tataagggat atatccctaa
     2221 tcacagaatt gtgacccgaa ggtattaatt gaaatgttac acgtgtgact tttgtgcatc
     2281 gccctgatgc agcattggcg ccacagcttc ggaccgatcg cgggcgacat cgtctcacgt
     2341 tatgagactt cgggggatgc ttgaccggtt gtggcggcac gcggagaa
//
LOCUS       DASYEG010000292        13516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_547421, whole genome shotgun sequence.
ACCESSION   DASYEG010000292 DASYEG010000000
VERSION     DASYEG010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 13516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13516
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547421"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VGM72_12360"
     CDS             <1..186
                     /locus_tag="VGM72_12360"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY4266105.1"
                     /translation="ILNYFMHRQGRVISQTELIEHLYPLEEERDPNTIEVYIGRLRRK
                     LGRDSIKTLRGLGYRFG"
     gene            183..1550
                     /locus_tag="VGM72_12365"
     CDS             183..1550
                     /locus_tag="VGM72_12365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY4266106.1"
                     /translation="MSVLLPRSLRARLVIGAFIWITVGVSAAGIFISALFRQHATELA
                     DSEMRAHLEELASLIDVSPQGEPELYRALSDPRFSQMGSGYSWQVSREGKQLIKSTSA
                     STDDLPIPADPLVNGEIRKLTVPSGRETMIVYETLFSPVSGSDPLRLQVNVDSGIIDR
                     VLRTFNISLAISMVLLAVALSTAAALQVVFGLQPMARLGRALRAIRAGKTTHLPRSFP
                     LEVQPVVDDLNSLIDVNGQMVVRARAQAGNLAHALKTPLAVLTDEAYQLQNRGQAEAA
                     QVILQQCQRMERQIDYQIARARAAASRSVPGVNAPLLPAVANIVSAMKRLYAAKELEY
                     AIAIAPDCIAMCDPMDLNEMLGNLIDNACKWSLKRVAIRGAVDETANQAVVAVEDDGP
                     GLPMDSREMVFQIGQRLDERVPGSGLGLSIVRDLAQLYGGEIRLEDSDLGGLRAMLRL
                     PRVVS"
     gene            complement(1547..2218)
                     /locus_tag="VGM72_12370"
     CDS             complement(1547..2218)
                     /locus_tag="VGM72_12370"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY4266107.1"
                     /translation="MIPSPLSAVIFDKDGLLLDTERIVREAMFASATDLGYRMDDTLF
                     LSLIGHARDANDRQLQAHFGADFPLSDYREKCRLYFEDLCRSEVPVKPGARELLSFLK
                     ARGIPAAVATSTGRARSEENLMRTGLAKFIAALVTRDDVERPKPHPECFLKAANLLGV
                     SPKQCLALEDSHHGIRAAHGAGMAAIMVPDLLEATPEIRILCAGVLPNLDAVRVRIAN
                     SLADS"
     gene            2339..3742
                     /locus_tag="VGM72_12375"
     CDS             2339..3742
                     /locus_tag="VGM72_12375"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY4266108.1"
                     /translation="MNEARIGGNPFTSGMADFVAGLTYDAIPDAVIARIKLLILDSLG
                     CAIFGADLEWSRILMETLARVDTSVGASVWGTARRLSAPHAVLVNGTLVQSFELDDVH
                     RQGVLHVGAVTLPPLFAVAEMRPGMSGRDFLAAAVAGYEIGPRVGMCMGQEHIVQGWH
                     SGATVGVFSAAAGAAAALKLPAEKIVHALGIAGTQAAGLMAAQFGAMVKRMHAGRSAQ
                     SGFYAALLAANGFTGISDVFENPYGGFCTTFSRSNDRFNREELTRGLGAHFETLRISL
                     KFYSCVSTNHTALDAIRNMQARRPFGARDVAAIVVRASQATCEHAGWPYKPEGMTSAQ
                     LNLSYCLATLLLEGDCFVEQFSENLLADPSRLALAAKVQVQEDPAITALGPKFRHMVH
                     VAVHLTDGTVLEESVEAPRGSERNFPSVEDVAAKFSKLTAQAIPAAQSRRIVEMVLDL
                     DRIGDIDGLLRALVRAA"
     gene            3895..5169
                     /locus_tag="VGM72_12380"
     CDS             3895..5169
                     /locus_tag="VGM72_12380"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4266109.1"
                     /translation="MRTAGGASHAVYYGWWVLAAAAVAEMLSIGSTSYAAGLFVLPLQ
                     HELSLSRAAANSSIPISFAGGALVSPLVGYLLDRISIQWVIGLGAIGLGLGFALISVT
                     SSIALMVLALFVPIAFGAVAIGPLTTSTLASRWFYRRRGSALGFAALATSAGGIVVVP
                     LLSLAIEDFGWRPALQCEAVFIVLIVLMAAFFVIRSGPADLDLQGHPENEGRPAADMP
                     HAQNQPVQMPPADGGVIFLPYREMLTALNFWTIAIALGSLVGISQAIVVTLVPYGTAL
                     GFAPVSAAFLISAFSISAAVVKVASGLLADIMQRRTIMLGATISIVLALLVLLEASTY
                     PSMLLGCCLAGIALGGILPSSSALVAACFGSPSFGRVMGAMYVVILGSSIAAAWFVGA
                     SFDRTGGYNVAFVTFLVIAGAATLSALLVRTP"
     gene            complement(5217..6251)
                     /locus_tag="VGM72_12385"
     CDS             complement(5217..6251)
                     /locus_tag="VGM72_12385"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY4266110.1"
                     /translation="MERAVPNAEHALNGTRRIIPDWEAVRVSFEVYRRGSFRAAAEHL
                     QMSVNALRRRVDLLEETLGVTLLTRHVDGVRITAEGEKVFAVASRMETASFDLLQARD
                     QTNPNISGEVRLAVTEGLGTFWIAPRLVEFQRANPNLLIDVNCAMKSADVLRLEADVA
                     IQITRPTALDLRMVKLGRLHLIPFASPNYLETFGRPKTVEDLVRHRILVQSDDNVQWR
                     DLYDKLFPGISPTAIVALRTNVSSAHYWSIAKGAGIGLLPTYAQWIGAPIVPLDLDIH
                     ETVDIWLTYHPDVKRIPRVSRLIEWTIQAFSPQKYPWFRDEFIHSNELKDVYRHEPLA
                     TMFTGSAAVA"
     gene            6473..7564
                     /locus_tag="VGM72_12390"
     CDS             6473..7564
                     /locus_tag="VGM72_12390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4266111.1"
                     /translation="MEKARAAIPGLTNDAVVKRVINHNPNAMWAIARKRKFDPSVPFG
                     DGFFAFLHLNKKGLFQLATNTLDTANPDLSLLAGPGERPAGIYTWAALAPGMLAGGIA
                     MVLEKLETPTYAGVDIFSRPNTADGFRFNETLGLKKGAKVGPIYAPQLYTFARSRQVA
                     PIYDSYNNEAGSRDLSVTVARTFEDLMRVASLRAAVYIGEQECPYEEEFDGNDLSATH
                     LIGYVGNEPAGCMRVRYFADFAKIERLAVRKEFRNTRLSFQLVRAGIKLCQMKGYRRL
                     YGHAQKRLVNFWSRFGFRPLEGAKEFVFSDFDYVEIVADIERDPDSITIGADPYVMIR
                     PEGRWHVPGVLERSANRQVTRPSVQERHS"
     gene            7561..8154
                     /locus_tag="VGM72_12395"
     CDS             7561..8154
                     /locus_tag="VGM72_12395"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEY4266112.1"
                     /translation="MNTVVNLKDFQERGRPLPTLLLIDLHHDVSDAIEPPREALSCEA
                     ISNCRTVLRHARACGIPIAFTRRVAAPANMMSSSVYPRWIEGIEPNRWDMVFDRQRPS
                     CYASAEFAEVADEIGGNYVIAGRFAEIACLSTAIDAFHRDHRPSVLMDALMCSAPGDL
                     PPAAMLRAVACILSQYAQTIRTQSWIHATSRRVGAAK"
     gene            8151..8330
                     /locus_tag="VGM72_12400"
     CDS             8151..8330
                     /locus_tag="VGM72_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266113.1"
                     /translation="MTAFTTLHPLKYIAEALSQMATTLRDFEMLESAQLLDKAKSDID
                     QKLIDRLKRGTAQKR"
     gene            8770..11133
                     /locus_tag="VGM72_12405"
     CDS             8770..11133
                     /locus_tag="VGM72_12405"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4266114.1"
                     /translation="MVHRGVAAAVLSFLWLPAAAWTSEAQAQTPQLETGQSEQVVVTA
                     TQRITPILDLPQSASVFTADRLDDAHVSQVKQLIALAPSMNVINSIGETFGQLIAVRG
                     VATSGADLGLESAVGVTVDGVPLMRPNLAVFDFQGVDRVEFLRGPQGTLFGTNTTAGI
                     INVLTRRPDFTPSFEASATLGERELRELRLSASGALIASKLAGRIDAVAGAVDGYLPN
                     PNTGEVYGARRREELRGQLFLVPTDDIDVRIIADYLHHGGSVNSPVYRIVGPTGAMVD
                     AISGVPLKASFHATDLAQIDAAAPRFETSDSAGVSAEVNWQTGPGRFTAIASYRSADA
                     RRNYDVDNSPANIANDPRDGESYGLGTLELRFQGKVDRFDYLFGAFAKRGRIVSRDSY
                     TVGPDYEPYTNALGGGNIPALTGLPEGSNDPAGSGVFDVYRQRESGYAFFTHEVIALT
                     DSLSLALGARYTAETKSLDAAITTNNPGCASAVALHGAALSGVPLGLQGLICIPNLDP
                     RYDGNYTSGTGEGNWSGTASLTQRLNPLLSAYAEFSRGYKAGGFQLDRSGMSPLSPSL
                     SELMFHQETADSIEAGLHAMSLDDRWGASAALFHTSFNNYQFSYFTGLNRHTRNVPRL
                     VTKGVEAEGAYRPLEDLEFSVSGIYQEAIFGASGFPSDLTQLESTTAPIAPRWVLVGA
                     ASYRRSFAGLGVTGFGGFDVRWQSKANVGVSATPTPGFLQDAYAVFGARIGVESPDRN
                     WRVEVWARNLFDQRAWSILNSTTLQPGSISGFVIDPRSTGVTATLMW"
     gene            11239..11382
                     /locus_tag="VGM72_12410"
     CDS             11239..11382
                     /locus_tag="VGM72_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266115.1"
                     /translation="MASVTAPAFYGDVAKSRQDIGFIVAMSSCGTRIGNELGGPPLVA
                     GQP"
     gene            11407..12747
                     /locus_tag="VGM72_12415"
     CDS             11407..12747
                     /locus_tag="VGM72_12415"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4266116.1"
                     /translation="MDAAFADIAAILDRRKLGAFQKFVIALCAAIVFVEGMNAQSAGY
                     VAPALRAAWGLSPSELGLFFSSGLTGLMLGGLVVAPLADRLGRRPILIGCVALFGICS
                     LGCAASTSIIMLDGLRFLTGLGIGGGMPNAIALTAEYSPLRRRSFMVAVMVTGFIFGS
                     IFAGLVAAPLVPVWGWQSAFVTGGVLPLLLLPVLYYTLPESIRFLVLHKGERAVIATL
                     IRRIDPAVRIDANTRLIVEEHGASGMGVPALFREGRARSTVLLWIIYFMSLLNLYLFA
                     SWLTTHVREAGMSEGTAILVGTLFQVGGAFGGVFGWIADKAGPSRAIFTAYLIGAAAI
                     ACIGFAQGDLMLLTVSVFAAGFGIIGGQTAANALAAMSYPTQIRSTGVGWATGIGRAG
                     SIVGPGLAGILVQLGMSTQNIFYLAVIPAIVASLAGASLNGLRRPLPSKENAAV"
     gene            12764..>13516
                     /locus_tag="VGM72_12420"
     CDS             12764..>13516
                     /locus_tag="VGM72_12420"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY4266117.1"
                     /translation="MLSKDDNERLTRTGPGTPMGDVFRRYWLPVLLASELSQPDGAPL
                     RVRLLGEDLVAFRDTGGKVGLVDAYCPHRRAPMFLGRNEQCGLRCVYHGWKFDTDGQC
                     LEMPTEPKASAYKDKVRIKSYRVWEGGGMIWAYMGPTAAMPAPPDMELTRAPDTHRFV
                     SRTVQDCNYLQALEGGLDSAHATIMHNVTIGDLSWLEDFEATTPRLDVELTDYGFQYS
                     GIRKQNGRHWVRVYQYIMPVTQIRGRIAPVRGE"
BASE COUNT         2336 a         4183 c         4269 g         2728 t
ORIGIN      
        1 atcctgaatt atttcatgca ccggcaggga cgggtgattt cgcagaccga actgatcgag
       61 catctttatc cgctggaaga ggagcgcgat cccaacacga tcgaggtcta tatcggccgg
      121 ttgcggcgca aattgggccg cgactccatc aagacgctgc gcggtctcgg atatcgcttc
      181 ggatgagcgt gctgcttccc cgctccttgc gcgcgcggct cgtcatcggc gcgttcatct
      241 ggatcacggt gggcgtttcg gccgcgggca tcttcatctc cgccttgttc cgccaacatg
      301 ccaccgagct cgccgattcg gaaatgcgcg ctcatctcga agagctggca tccctcatcg
      361 atgtcagccc gcaaggcgaa cccgaattgt atcgcgccct cagcgatccg cggttttcgc
      421 agatgggatc gggctattcc tggcaggtga gccgcgaggg caagcaactg atcaaatcca
      481 cctcggccag taccgacgat ctgcctatcc cagccgatcc gctcgtcaat ggagagatca
      541 gaaaactcac cgtgccgagc ggacgcgaga ccatgatcgt ctacgaaacc ttgttctcgc
      601 cggtcagcgg gagcgatccg ctgcggctgc aggtgaatgt cgattccggg atcatcgacc
      661 gcgtgctgcg gaccttcaac atctcgctgg ccatttcgat ggtgctgctg gccgtcgcgc
      721 tgagcacggc ggcggccttg caggtggtgt tcggtcttca gcccatggcg cggctcggtc
      781 gcgcgctgcg cgccatccgc gcgggcaaga cgacccatct gccgaggagc ttcccgctgg
      841 aggtgcagcc ggtggtggac gatctcaaca gcctgatcga cgtgaacggc cagatggtgg
      901 tgcgcgcccg tgcgcaggcg ggcaacctcg cgcacgcgct gaagacgccg ctcgcggtgc
      961 tgaccgacga agcctaccaa ctccagaatc gcggacaggc ggaggccgcg caagtgattc
     1021 tgcagcaatg ccagcgcatg gaacgtcaga tcgattacca gatcgcgcgg gcccgcgcgg
     1081 ctgcgtcgcg ttcggttccg ggagtcaacg cgcctttgct gccggcggtc gccaatatcg
     1141 tcagcgccat gaagcgtctc tacgccgcga aggagctgga gtatgcgatc gccattgcgc
     1201 ccgattgcat cgcgatgtgc gacccgatgg atttgaacga gatgctcggc aacctcatcg
     1261 acaatgcctg caaatggagc ctcaaacgcg tcgccatcag gggggcggtc gatgagacgg
     1321 ccaaccaggc cgttgtcgcg gtcgaagatg acggtccggg tctgcctatg gactcgcgcg
     1381 agatggtttt ccagatcgga cagcgcctcg acgaacgcgt gcccggctcc ggcctcggcc
     1441 tctccatcgt gcgcgatctg gcgcagcttt atggcggcga gattcgcctg gaggattccg
     1501 atctcggggg cctgagagcc atgctgcgcc ttccccgcgt ggtttcctaa ctatcggcca
     1561 atgaattggc gatgcgtacg cgcactgcgt cgagattggg aagcaccccc gcacacagaa
     1621 tccgaatctc cggtgtcgcc tccagcaaat cgggcaccat gatcgcggcc atgcccgcac
     1681 catgggcggc ccggatgccg tgatgcgaat cttccagcgc caggcattgc tttgggctca
     1741 cgcccagcaa attcgccgcc ttgaggaagc attccggatg gggcttgggg cgctcgacat
     1801 cgtcgcgggt gacgagggcg gcgataaatt tcgccagtcc cgtccgcatc aggttttctt
     1861 cgctgcgcgc gcgtcccgtc gaggttgcga ccgcggccgg aatgccgcgc gccttgagga
     1921 agctcagcaa ttcgcgggcg ccgggtttga cgggcacctc gctccggcag aggtcttcga
     1981 aatacagccg gcatttctcg cgatagtccg aaagcgggaa atccgccccg aaatgcgcct
     2041 gcaactgccg gtcgttggcg tcgcgcgcat gcccgatcag gctgaggaac aacgtatcgt
     2101 ccatgcgata gccaaggtct gtggcggacg cgaacatcgc ctcgcgcacg atgcgttcgg
     2161 tatcgagcag gagtccgtcc ttgtcgaaga tgacggcgga caagggtgag ggaatcatcg
     2221 ccaggttccg ggctgctgcg aatcactcta acgcggccct ttggcgtgcg catccttcgt
     2281 ttccggcgtg cgacccgata tggtcggcgg cgcaatagcc atcaccggta gagacatcat
     2341 gaatgaggcc agaatcggcg ggaatccctt cacctccggc atggccgatt tcgtcgccgg
     2401 gctcacctac gacgccattc cggacgccgt catcgcgcgc atcaagctgc tgatcctgga
     2461 ttcgctcggc tgcgcgatct tcggcgccga cctggaatgg agccgcatcc tgatggagac
     2521 gctggcgcgg gtggacacct ccgtcggagc gagtgtgtgg ggcaccgcgc ggcggctgtc
     2581 cgcgccccac gccgttctgg tcaacggaac gctggtgcag agcttcgagc tggacgacgt
     2641 ccatcggcag ggcgttctgc atgtcggggc cgtgacgctg ccgcctctct tcgccgtggc
     2701 ggagatgcgg cctggcatga gtggccgcga ttttctcgct gccgcggtgg cgggttatga
     2761 aatcggtcca cgcgtcggca tgtgcatggg acaggagcac atcgtccagg gctggcattc
     2821 cggtgcgacg gtcggggtct tttccgccgc cgccggggcg gccgccgcgt tgaagctgcc
     2881 ggcggagaaa atcgtccacg cgctcggcat cgccggaacc caggccgccg gcctgatggc
     2941 cgcgcagttc ggcgccatgg tcaagcgcat gcatgccggg cgttccgcgc aaagcggatt
     3001 ttatgcggcg cttctggccg cgaacggctt caccggcatc agcgatgtgt tcgaaaaccc
     3061 ctatggcgga ttttgcacga cgttttcgcg ctcgaacgac cgcttcaacc gcgaagaatt
     3121 gacgcgcggg ctgggcgccc attttgaaac cctgcgcatc tcgctcaaat tctattcctg
     3181 cgtcagcacc aatcacacgg cgctcgatgc cattcgcaac atgcaggcgc gccgtccctt
     3241 cggcgcgcgc gatgtcgccg ccatcgtcgt gcgcgcctcg caggcgacct gcgaacatgc
     3301 gggctggccc tacaagccgg aaggcatgac gtcagcccaa ctcaacctct catattgcct
     3361 ggcgacgctc ctcctcgaag gcgattgctt cgtcgagcaa ttttcggaaa acttgctggc
     3421 cgatccgtcc cgtctcgcgc tcgccgccaa agtccaggtg caggaagatc ccgcgatcac
     3481 cgcgctggga ccgaaattcc gccacatggt tcacgttgcg gtacatctca ccgacggtac
     3541 ggttttggaa gaaagcgtgg aggccccgcg cggcagcgaa cgcaacttcc ccagcgtgga
     3601 ggatgtcgcc gccaaattct caaagctgac cgcccaggcc attcctgccg cgcaaagccg
     3661 gcgtatcgtg gaaatggtgc tggacctcga caggattggc gacatcgatg ggctgttgcg
     3721 ggcgctggtc cgcgccgcct gagtcgctag cctgagtcgc gaggctgagt cgccaacttg
     3781 tcgttcgcat ctctttcacg ctacgctgcg cgcgataggg ggagcagcgc gatcggcgtc
     3841 aaacgccgcg ctggcctagg tttgagcgtg catgagccaa tagcggacaa ggcgttgcgt
     3901 acagcaggcg gtgcgtccca cgcggtctat tatggctggt gggtgctggc ggctgcggcc
     3961 gttgccgaaa tgctttctat cggcagcacg agctatgccg ccggtttgtt cgtgcttccc
     4021 ctgcaacatg agctgtcgct cagccgcgcc gcggcgaaca gttcgattcc catctccttt
     4081 gccggcgggg cgttggtgtc tcctctggtg ggttacctgc tggaccgcat ttccatccaa
     4141 tgggtgatcg gcctcggcgc gattggcctc ggtctgggtt tcgcgctgat ttccgtgaca
     4201 tcctcgattg cgctgatggt gctggcgctg ttcgtgccca tcgcgttcgg cgcggtggcc
     4261 atcggcccgc tgaccacctc gacccttgct tcgcgctggt tttaccggcg gcgcggcagc
     4321 gcgctgggct tcgccgcatt ggcgacatcg gcgggcggca ttgtcgtcgt gccgctcttg
     4381 agcctcgcca tcgaggattt cggctggcgc ccggcactgc aatgcgaagc ggtgttcatc
     4441 gtcctgatcg tgctgatggc ggcgttcttc gtcatccgca gcggaccggc cgatctcgat
     4501 ctccaaggtc atcccgaaaa cgaagggcgc cccgcggccg acatgcctca cgcccaaaac
     4561 cagccggtcc agatgccgcc ggccgatggc ggtgtcatct tcttgcccta tcgcgagatg
     4621 ctgacggcgc tgaatttttg gaccatcgcc atcgcgctcg gctcgctcgt cggcatcagt
     4681 caggcgattg tcgtgacgct ggttccctac ggcacggcct tggggttcgc cccggtctcc
     4741 gccgcgttcc tgatttccgc attctcgatt tcggcggctg tcgtcaaagt ggcgagcggt
     4801 ctgttggccg atatcatgca gcgccggacg atcatgctgg gcgccacgat ttcgatcgtc
     4861 ctggcgctgt tggtgctgct cgaggcatcc acctatccgt cgatgctgct cggctgctgc
     4921 ctggcgggaa tcgcgttggg cggcattctt ccctcgtcgt cggcgctggt ggccgcgtgt
     4981 ttcggctcgc cttcctttgg ccgggtcatg ggcgcgatgt atgtggtgat cctgggctcg
     5041 tccatcgccg cggcttggtt cgtgggggcg agtttcgacc gcaccggagg gtataatgtc
     5101 gcatttgtga catttctggt catcgcaggg gccgcgacgc tctcagcgct tttggtgcgt
     5161 acgccttagg gataggcgcg gaacggcaag acggcccggg gacgcgcccg cgccgttcaa
     5221 gccaccgccg ccgaaccggt gaacatggtc gccagcggtt cgtggcgata gacgtccttc
     5281 agttcgttcg aatggatgaa ctcgtcgcgg aaccaaggat atttctgcgg cgagaacgcc
     5341 tggatggtcc attcgatcag ccgtgacacg cgcgggatgc gcttcacgtc gggatgatag
     5401 gtcagccaga tatccaccgt ctcatggata tcgagatcca gaggaacgat cggcgcgccg
     5461 atccactgcg cataggtggg cagaagtccg atgcccgcgc ccttggcgat ggaccagtaa
     5521 tgcgcgctgc tgacattggt tctgagcgcc acgatcgcgg ttggcgagat gccgggaaac
     5581 aatttgtcgt agaggtcgcg ccattggacg ttgtcgtccg actgaacgag gatgcgatgg
     5641 cgcacgaggt cctcaacggt cttgggacgg ccgaacgttt cgagataatt gggtgaagcg
     5701 aacggtatga gatgcagccg gccgagcttg accatccgca ggtcgagtgc tgtggggcgc
     5761 gtgatctgga tcgcgacatc ggcttccagg cgcaaaacat ccgccgattt catcgcgcaa
     5821 ttgacatcga tcagcagatt gggattggct cgctgaaact cgacgagtcg cggcgcgatc
     5881 cagaaggtcc ccaaaccttc cgtcactgcg aggcgcactt cgcccgagat attgggattg
     5941 gtttgatcgc gcgcctgcaa aaggtcgaac gatgcggttt ccatccggct tgcgacggcg
     6001 aaaacctttt cgccttccgc ggtaatgcgc acgccgtcga cgtggcgcgt cagcaatgtg
     6061 actccaaggg tctcctcgag caggtcgacg cgccggcgca gcgcattgac ggacatctgt
     6121 agatgctcgg ctgcggcgcg gaagctgcct cggcggtaca cctcgaacga aacgcgcaca
     6181 gcctcccaat cgggaatgat tcgccgggtt ccattcagtg cgtgttccgc attcggaaca
     6241 gcccgttcca tttcgctatg catacttcat caccgtccct cagtaagttt ttatcacgga
     6301 tcattgaaaa agtggggtga aaaaaatgac cgaacatccg ttagatctta tccgggccca
     6361 ggcgcgagtc atcgaggcaa agccgagaca ccccctgggg actcttgatt cagatagatt
     6421 ttccaaacac ttagtagtat tccggcccaa tgaagccgcc gtagacgatt tgatggaaaa
     6481 agcccgcgcc gccattccgg gcctcacaaa cgatgcggtt gttaaacggg tcattaacca
     6541 caatcccaat gccatgtggg cgatcgcgcg caagcgcaag ttcgatccga gtgttccgtt
     6601 tggagatggc ttctttgctt tcctccacct gaacaagaaa ggtttgttcc agttggcgac
     6661 gaatacgctc gataccgcca acccggattt gtcccttctc gccggtcccg gcgaacggcc
     6721 tgccgggatc tacacctggg cggcattggc gccgggcatg ctcgccggtg gcatcgctat
     6781 ggtgctggaa aagctggaaa ccccgaccta tgccggcgtc gatatcttct cgcgtccgaa
     6841 cacggccgac gggtttcgct tcaacgagac gctggggctt aaaaagggcg ccaaggtcgg
     6901 cccgatctac gcgccccaac tttatacgtt tgcgcgttcg cgccaggtcg cgccgatcta
     6961 cgacagctac aacaatgagg ccggcagccg cgatctttcg gtcacggtgg cgcgcacatt
     7021 cgaagatctc atgcgcgtgg cgagcctcag ggccgcggtc tatatcggcg agcaggaatg
     7081 tccgtatgaa gaagaattcg acggtaacga cctctccgcc acccacctga tcgggtatgt
     7141 cggcaacgag ccggcgggct gcatgcgggt gcgctacttc gccgatttcg cgaagatcga
     7201 acgtctcgcg gtgcgcaagg aattccgcaa tacgcggctc tccttccaac tggtgcgggc
     7261 cggaatcaaa ctctgccaga tgaaaggcta tcgccggctt tacggtcatg cgcaaaagcg
     7321 tctggtcaat ttctggagcc gcttcggttt ccggccgctg gaaggcgcca aggagttcgt
     7381 cttctcggat ttcgactatg tcgagatcgt cgcggacatc gagagggatc cggattccat
     7441 cacgatcggc gccgatccct atgtgatgat caggcccgaa ggccgctggc atgtgcctgg
     7501 cgttctcgag cgctcagcca accggcaagt gacgcgtccg tcggtacaag aacgccattc
     7561 atgaatacgg tcgtcaacct caaggatttc caggagcgtg gacgtccgct gcccacgctc
     7621 ctgcttatcg atttgcatca cgacgtctcc gatgccatcg agccgcctag ggaagcgctt
     7681 tcgtgcgaag cgatttccaa ctgccgcaca gtgctgagac atgcgcgtgc ctgcggtatc
     7741 cccatcgcgt tcacgcgccg ggtcgcggcg cccgcgaaca tgatgagttc gtccgtctat
     7801 ccgcgctgga tcgaagggat cgaacccaac cggtgggata tggtcttcga ccgccagcgg
     7861 ccgtcctgct atgcgagcgc ggaattcgcg gaagtcgcgg acgaaatcgg cggcaattac
     7921 gtcatcgccg gaaggtttgc ggaaattgca tgtctctcga ccgcaataga tgctttccac
     7981 cgcgatcatc gtccttctgt attgatggac gcgttgatgt gcagcgcgcc cggcgatttg
     8041 ccgcccgccg cgatgctccg ggccgtggcc tgcatccttt cgcaatatgc gcagacgata
     8101 agaacacagt cctggataca cgccacctca cggcgagtgg gagcggccaa atgacggcgt
     8161 tcacaacctt gcatccgctc aaatacatcg ccgaagcgct gtcgcagatg gcgacgacgc
     8221 tcagggattt cgaaatgctg gaatcggccc agctcctcga caaggcgaaa tcggatatcg
     8281 accagaagct gatcgacaga ttgaaaaggg ggacggcgca gaaacggtga ggaacgctcc
     8341 gcatacggca taattgccgg accgcatgcg aaaaaagcgg cacaagcagt ttgtcttggc
     8401 catgccatgg ttcacaaggt cgcgctcgag ctgccttctc gtgagccatc agtggccggg
     8461 gcggggcggt cgactagtcc ccctggtcga ccgcctccat ttcaggccgg acgcgagtgc
     8521 caaaagcgct cgtctctggc ccgaacatgc tgttttagga tcgaaatccc gtcatgcacc
     8581 attcactttc tttattcagc ttaacggcgg attctgacgc ggcaccatac ggattcatta
     8641 ctccggctgc cgtatcggct caatggcaag ttgaatttct cgtgcccggc cgccgtcaac
     8701 acgcatgttg cgggtttttc cgctgaggta tcatatgctt gggtcgtgta caggtgtttg
     8761 gaggggacgt tggtccaccg gggggtggct gctgcggttc tgagtttttt gtggttgccc
     8821 gcggcggcct ggacgagtga ggcgcaagcc cagactccgc agcttgaaac ggggcagagc
     8881 gaacaggtcg tcgttacggc gacacagagg atcacgccaa tcctcgacct gccgcaaagc
     8941 gcgtcggtgt tcacagccga caggctggat gatgcccatg tcagccaagt caaacaactg
     9001 atcgcgctcg ctccgtcgat gaacgtgatc aattcgattg gcgagacctt cggccagctc
     9061 atcgcggtgc gcggggtcgc cacctcgggc gccgatctcg ggctcgaatc ggcggtcggg
     9121 gtcaccgtgg acggggtgcc tctgatgcgg cccaacctgg cggttttcga ttttcagggt
     9181 gtggacaggg tggagttcct gcgcggcccc caagggacgc tgttcggcac gaacaccacc
     9241 gcgggcatca tcaatgttct gacccgccgg ccggatttca cgcccagctt cgaggcgagc
     9301 gccacgctgg gcgagcggga gctgcgcgaa ttgcgcctct ccgccagcgg cgcgctgatc
     9361 gcatcgaagc tcgcaggccg catcgatgcc gtggccggag cggtcgatgg atatcttccc
     9421 aatcccaata cgggagaagt ctatggcgcg cggcgccgcg aggagctgcg cggccagttg
     9481 ttcctggtcc ctaccgacga catcgatgtg cgcatcatcg ccgattatct tcatcatggc
     9541 ggttcggtga attctcccgt ttaccggatc gtgggaccga ccggcgcgat ggtcgacgcg
     9601 atcagcgggg tgccgctgaa ggcatcgttc catgccaccg atcttgccca gatcgatgcc
     9661 gccgcgccgc gtttcgagac cagcgacagc gccggcgtaa gcgcggaagt gaactggcag
     9721 accggccccg gacggttcac cgccatcgcc tcctaccgca gcgcggacgc acggcggaat
     9781 tacgatgtcg acaatagtcc cgccaacatc gcaaacgatc cgcgcgatgg ggaaagctat
     9841 gggctgggaa cgctggagct gcggtttcag ggcaaggtgg atcgtttcga ttatctgttc
     9901 ggcgcgtttg ccaagcgcgg ccgcatcgtg tcgcgcgatt cttacaccgt tgggcccgat
     9961 tacgagccgt atacgaacgc gctcggaggc ggaaacattc ctgccctcac aggtcttccg
    10021 gagggcagca acgatccggc cggcagtggc gttttcgatg tctaccggca gcgcgagagc
    10081 ggctatgcct tcttcaccca tgaagtgatc gcgctgacgg acagtctctc gctcgccctg
    10141 ggagcgcgtt acacggccga gacaaaatcg ctggatgccg ccatcaccac caacaatccg
    10201 ggctgcgcca gtgcggtcgc ccttcacggg gcggcgcttt cgggcgttcc cttgggacta
    10261 cagggtttga tctgcatccc gaatctcgat ccgcgctatg acggcaatta cacatcgggc
    10321 acgggcgaag gaaactggtc gggaacggcg tccctgacgc aaagactgaa tccgttgctg
    10381 agcgcctacg ccgaattcag ccgcggctac aaggctggcg ggttccagct cgaccgctcg
    10441 ggcatgtctc cgctctcgcc ctcgctttcc gagctgatgt tccaccagga aacggccgat
    10501 tccatcgagg ccggcctgca tgcgatgtcc ctcgatgaca ggtggggcgc cagtgcggct
    10561 ctctttcaca ccagcttcaa caattatcag ttcagttatt tcaccggcct caatcgccat
    10621 accaggaatg tgccgaggct cgtcaccaag ggcgttgagg cggaaggcgc ataccggccg
    10681 ttggaggatt tggagttttc cgtctccggc atttatcagg aggcgatttt cggcgcgtcc
    10741 ggttttccat ccgatctgac gcagcttgag agtacgaccg cgccgatcgc accgcgttgg
    10801 gtgcttgtgg gcgcggcatc ctataggcgg tcttttgccg gcttgggcgt aacgggtttc
    10861 ggcggtttcg atgtgcgctg gcaatccaaa gccaatgtcg gcgtatcggc cacacccacg
    10921 cccggttttc ttcaggatgc ctatgccgtg ttcggggctc gcatcggcgt ggaaagtccg
    10981 gacaggaatt ggcgtgtgga ggtgtgggcc cgcaatctgt tcgatcaaag ggcgtggtcg
    11041 atcctcaaca gcaccacgct ccagcccggc tcgatctcgg gctttgtcat cgatccgcgc
    11101 tcgaccggtg tcaccgcgac cctcatgtgg tagagacccc ggccgtccgc gcgtggcctg
    11161 tcacgggtgt tgccgccgct tgccgcgcgc ggggggcgag cttacgctcc ggccaatgaa
    11221 ttgggagcgg gagcatccat ggcgagcgtg acggcaccgg ccttctatgg cgatgtggcg
    11281 aagtccaggc aggacatcgg tttcatcgtc gcgatgtctt catgcggcac gcggatcggg
    11341 aacgaactgg gcggtccgcc gctcgtcgcc ggtcagccct gatcgaaggg ccgcgagctg
    11401 caaatcatgg acgctgcgtt tgccgatata gccgctattc tcgaccggcg aaaactgggc
    11461 gctttccaga aattcgtcat cgcgctctgc gccgcgatcg tgtttgtcga aggcatgaac
    11521 gcgcaatcgg ccggttatgt cgcgccggcc ttgcgcgcgg cctggggact aagcccttcc
    11581 gagctgggat tgtttttctc gagcggcctc accggactga tgttgggcgg cctcgtcgtg
    11641 gcgccgcttg ccgaccggct gggacgccgt cccatcctga tcggctgcgt cgccctgttc
    11701 ggaatctgca gcctcggctg cgcggcgagc acgtccatca tcatgctgga tggattgcgc
    11761 ttcctcaccg gcctgggcat cggcgggggg atgcccaacg cgattgcgct caccgccgaa
    11821 tattcgcctc tgcgccggcg cagcttcatg gtggctgtga tggtcaccgg cttcatcttc
    11881 ggctcgatct tcgcgggcct cgtcgccgcg ccgctggtgc ccgtctgggg ctggcaatcc
    11941 gcgtttgtga ccggcggggt gctgccgctg ctgctgttgc cggttctcta ctatacgctg
    12001 cccgaatcca tccgcttcct ggtgctgcac aagggcgagc gcgcggtcat cgccacgctg
    12061 atccgccgca tcgatcctgc ggtgcggatc gatgcgaata cgcgcctgat cgtcgaggaa
    12121 catggggcgt cgggcatggg cgtgcctgcc ttgttccgcg agggccgcgc gcgttcgacg
    12181 gtgcttctgt ggatcatcta tttcatgagc ctgctcaatc tctatctgtt cgcgagctgg
    12241 ctcacgaccc atgtgcgcga agcgggcatg tcggaaggca ccgcgatcct ggtgggcacc
    12301 ctgttccagg tgggcggggc cttcggcggg gtgttcggct ggatagcgga caaggccggg
    12361 cccagccggg ccatcttcac cgcctatctg atcggcgcgg ccgccatcgc ctgcatcggc
    12421 tttgcgcaag gcgatctcat gctgttgacg gtttcggtgt ttgccgcggg cttcggcatc
    12481 atcggtggac agacggccgc aaatgcgctt gccgcgatgt cctacccgac ccagatccgc
    12541 tccaccggag ttggctgggc caccggcatc gggcgggcgg gttccatcgt cggtccgggg
    12601 ctcgcgggaa tcctggtcca gctcggcatg tcgacgcaga atattttcta tctcgcggtc
    12661 attccggcga ttgtcgcttc gctcgcgggc gcgagcctga atggattgcg gcggccgctt
    12721 ccgtccaagg aaaatgcggc cgtgtgaagt ccgagggtac ctgatgcttt ccaaagacga
    12781 caatgaacgt ctgacgcgca ccggacccgg cacgccgatg ggcgatgtgt ttcggcgcta
    12841 ttggttgccg gtgcttctgg caagcgaact gtcgcagccc gacggcgcgc cgctgcgcgt
    12901 gcgcctgctc ggcgaggatt tggtcgcgtt ccgcgacact ggcggcaagg tggggctggt
    12961 cgatgcgtat tgtccgcacc gccgcgcgcc gatgtttctc ggccgcaacg agcaatgcgg
    13021 cctgcgctgc gtctatcatg gctggaaatt cgatacggac ggccaatgcc tggagatgcc
    13081 gacggagccc aaagcaagtg cctacaagga taaagtccgc atcaaatcct atcgcgtctg
    13141 ggaaggcggc ggcatgatct gggcctatat gggcccgacg gccgcgatgc cagcgccacc
    13201 cgatatggag ttgacgcgcg cgcccgacac gcatcgcttc gtctcgcgca ctgtgcagga
    13261 ttgcaattat ctccaggcgc ttgaaggcgg tctcgactcc gcgcacgcca ccatcatgca
    13321 caatgtcacc attggcgatc tgtcgtggct cgaagatttc gaggccacca cgccccgtct
    13381 tgacgttgaa ctcaccgatt acggtttcca atatagcggc atccgcaaac agaacggccg
    13441 tcactgggtg cgggtctatc aatacatcat gccggtgacg caaatccgcg gacgcatagc
    13501 gccggtgcgc ggtgag
//
LOCUS       DASYEG010000293         6428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3737006, whole genome shotgun sequence.
ACCESSION   DASYEG010000293 DASYEG010000000
VERSION     DASYEG010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6428
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3737006"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /gene="thiS"
                     /locus_tag="VGM72_12425"
     CDS             complement(<1..410)
                     /gene="thiS"
                     /locus_tag="VGM72_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004894161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEY4266118.1"
                     /translation="MTVEALLTHLALDPRKVAVERNLEIVPRSTYAAVDLSEGDRLEI
                     VHFIGGGNMAAINPAVDKPFVVAGRTLRSRLIVGTGKYKDYAQNAAALEASGAEMVTV
                     ALRRVNVMEPGAPKLIDFIDPKKYVYLPNTAGCFT"
     gene            579..1019
                     /gene="aroQ"
                     /locus_tag="VGM72_12430"
     CDS             579..1019
                     /gene="aroQ"
                     /locus_tag="VGM72_12430"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003806.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEY4266119.1"
                     /translation="MILPVYVLNGPNLNLLGIREPEIYGRATLGDIEQAVVGRAKTHG
                     LDIVFRQSNHEGVLVDWIQEARTKASGVIVNPSAFSHTSVAIYDAFKALDKPIVEVHL
                     SNPRQRETFRHRDYVSEVAKGVIEGFGMSGYLLAVDAMAELIKT"
     gene            1016..1513
                     /gene="accB"
                     /locus_tag="VGM72_12435"
     CDS             1016..1513
                     /gene="accB"
                     /locus_tag="VGM72_12435"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEY4266120.1"
                     /translation="MTDKKQQSLGGRIGKAISGESAPSVDADMVRELAKLLEETGLTE
                     LEIEQGGVRVRVARGAMLAASAVPAMVPVGPAPAHETPAEPARRAGTVVTSPMVGTVY
                     LSPQPGSPAFVKIGDAVTDGTLCIIEAMKTMNPVPSPAGGRVAEIYVRDGQPVEFGEP
                     LMLIE"
     gene            1521..2876
                     /gene="accC"
                     /locus_tag="VGM72_12440"
     CDS             1521..2876
                     /gene="accC"
                     /locus_tag="VGM72_12440"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY4266121.1"
                     /translation="MFEKVLIANRGEIALRVNRACKEMGIATVAIHSTADADAMHVRL
                     ADESVCIGPPPANQSYLNIPAIISACEITGADAIHPGYGFLSENAHFADIVKAHGITF
                     IGPQPAHIRLMGDKIVAKQTVKSLGIPTVPGSEGGISSESEAVAVANTIGYPVLIKAS
                     AGGGGRGMKVARSASELGLALSTARAEAKAAFGDDDVYMEKYLAKPRHVEIQVIADSH
                     GKVIHLGERDCSLQRRHQKVWEEAPSPALNVEGRNQIGGVVTAALTKLGYLGVGTVEF
                     LYENGQFYFIEMNTRLQVEHPVTELVTGLDLVREQIRIADGGALEIDQSDVVVAGHAI
                     ECRINAENPFTFQPSPGRITDFHAPGGLGVRMDSAAYAGYSIPPYYDSLIGKLIVHGK
                     SRNECLMRLRRALDELVIGGIETTIPLFQELIKQPDILDGDYNIHWLEHYLARRATGA
                     L"
     gene            2933..3319
                     /locus_tag="VGM72_12445"
     CDS             2933..3319
                     /locus_tag="VGM72_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY4266122.1"
                     /translation="MFRWLKRWSPQLLSLMRIAVGLTFLEHGTQKLLNFPVPRPGLAL
                     PLLLFTGILETVAGALVTLGLMTRLAAFLLSGELAVGYWWIHEPRSPYPMANGGEAMV
                     IYCFVFLYLAAAGAGPLSLDSLTRRA"
     gene            3316..4059
                     /gene="aat"
                     /locus_tag="VGM72_12450"
     CDS             3316..4059
                     /gene="aat"
                     /locus_tag="VGM72_12450"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEY4266123.1"
                     /translation="MISVTAINPEELLLAYRLGIFPMAESRTAKDVLWVRPHERGILP
                     LDKFHVPKRLARVVRSNRFHITADAAFEDVIRACAESRPERRETWINDAILDVFISLH
                     KRGFAHSIETWHGDRLVGGLYGLSLSAAFFAESKFSRATDASKVALVHLAARLKAGGY
                     TLLDAQFPNPHLEQFGAATVTEERFQFMLARALAASGDFYGLGRAGVAPDGAAGAPVT
                     EGGDGGAVPAAGADSVAGSSVLQVITHTS"
     gene            complement(4095..4379)
                     /locus_tag="VGM72_12455"
                     /pseudo
     CDS             complement(4095..4379)
                     /locus_tag="VGM72_12455"
                     /inference="COORDINATES: protein motif:HMM:NF021444.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
     gene            complement(4460..4921)
                     /gene="mlaD"
                     /locus_tag="VGM72_12460"
     CDS             complement(4460..4921)
                     /gene="mlaD"
                     /locus_tag="VGM72_12460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEY4266124.1"
                     /translation="MQNSIVETLIGAAVVAVAALFLVFAYSSTGSGPVSGYDVIAKFS
                     KADGVNVGTDVRLSGIKVGTVEKLALDPKTYNAVVTLALENSIKLPDDSSVRITSEGL
                     LGNQYLSIEPGASMQPIKPGGEIENTQGSIDLIGLLGKAVFSTGATPGGGK"
     gene            complement(4994..5374)
                     /locus_tag="VGM72_12465"
     CDS             complement(4994..5374)
                     /locus_tag="VGM72_12465"
                     /inference="COORDINATES: protein motif:HMM:NF006040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HEY4266125.1"
                     /translation="MSFLSRLFGWWENATPGTYLTTRFSGIPVGSDDFGNRYYQTKDG
                     KRRWMIFAGTIEASRVPPDWHGWLHHTFALPPTEDPPKRKPWELDHEPNLTGTRGAWH
                     PDGSLWKDGQRPPATGDYDAWRPG"
     gene            5823..>6428
                     /locus_tag="VGM72_12470"
     CDS             5823..>6428
                     /locus_tag="VGM72_12470"
                     /inference="COORDINATES: protein motif:HMM:NF020060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266126.1"
                     /translation="MRITRHFTTEGKSPYEGIAFRTTTSEIRNPDGSVVFRHEGIEVP
                     EAWSQVACDVMAQKYFRRAGLAVCLKRVEENNVPPFLWRHVPHEEALAGLPATVKKAT
                     GEVSAKQVFHRLAGTWTYWGWKSGYFDSEKDARAFYDEMCHMLAAQKAAPNSPQWFNT
                     GLHWAYGIDGPSQGHYYVDFQTGELVRSASAYEHPQPHACFI"
BASE COUNT         1227 a         2005 c         1952 g         1244 t
ORIGIN      
        1 gtgaagcagc ccgcggtgtt gggcagatag acatatttct tgggatcgat gaaatcgatc
       61 agcttgggcg cgccaggctc catcacattc acccggcgaa gcgccactgt caccatctcc
      121 gcgccggagg cttcaagggc ggcagcgttc tgcgcgtaat ccttatactt gcctgtgccc
      181 acgataagcc gcgagcgcaa ggtgcggccc gccaccacaa agggcttgtc cacggccgga
      241 tttatggccg ccatgttgcc gccaccgatg aaatggacga tttccagccg gtcgccttcg
      301 gacaggtcca ccgccgcata ggtcgagcgg ggcacgatct caagattgcg ttccaccgcc
      361 actttcctcg ggtcgagcgc gagatgcgtg agcagggctt cgaccgtcaa agggctggta
      421 aattcccggg gctctccgtt aagacttaaa cgcaacgcca ttgacggctc tttctcctgt
      481 gtttccgttt tccgcagcct gaccgtataa cccgcttcgc aatccgccgc ttcgacccat
      541 tccttacgcc attaccgcat tttcgggggc agccgtccat gatcctaccg gtatatgtcc
      601 ttaacggacc gaacctcaat ctcctgggga tacgggaacc ggaaatttat ggccgcgcca
      661 cgctaggcga tatcgagcag gcggttgtgg gccgcgccaa gactcatggg ctggacatcg
      721 tcttccgcca gtccaaccat gaaggcgtgc tggtcgattg gatccaggag gcccgcacca
      781 aggcatcggg cgtgatcgtc aatcccagcg ccttttcgca cacctccgtc gccatttatg
      841 acgcgttcaa agcgctggat aaaccgatcg tcgaggtgca tctgtccaat ccgcgtcagc
      901 gcgaaacgtt ccgccatcgc gactatgtga gcgaggtggc gaagggtgtc atcgagggct
      961 tcggcatgag cggttatctg ttggcggtcg atgccatggc ggagctcatc aagacatgac
     1021 ggacaagaag caacaatcct tgggcggccg catcggcaag gcgatctcgg gagaaagcgc
     1081 gcccagtgtc gatgcggaca tggtgcgcga actcgccaag ctgctggaag aaaccgggct
     1141 caccgaactc gagatcgaac agggaggtgt tcgcgtgcgc gtggcgcgcg gcgccatgct
     1201 ggctgcttcc gccgtgccag ccatggtccc ggtggggccg gcacctgcac acgagacgcc
     1261 cgccgaaccc gcgcgccgcg cgggaacggt cgtcacctcg cccatggtcg gaacggtcta
     1321 tctctcgccc cagcccggct cgcccgcctt cgtcaagatc ggcgatgccg tgaccgacgg
     1381 cacgctgtgc atcatcgagg cgatgaagac gatgaatccg gtcccctcgc ccgccggcgg
     1441 gcgtgtcgcg gagatctatg tgcgcgacgg acaaccggtc gagttcggtg aaccgctgat
     1501 gctgatcgag tgatttctcg atgttcgaaa aagtcctgat tgcgaatcgc ggcgagattg
     1561 cgctccgcgt gaaccgcgcc tgcaaggaaa tgggcatcgc caccgtcgcg atccattcca
     1621 ccgctgatgc ggacgccatg catgtgcggc tcgccgatga atcggtctgc atcggaccgc
     1681 cgcctgccaa ccagtcctat ttgaacattc ccgcgatcat ctcggcctgt gagatcaccg
     1741 gcgcggacgc catccatccc ggctacggat ttctctccga gaatgcgcat ttcgcggaca
     1801 tcgtgaaggc ccacggcatc accttcatcg gcccgcaacc ggcgcatatc cggctgatgg
     1861 gcgacaagat cgtcgccaag cagaccgtga aatcgctcgg cattccaacc gttcccggtt
     1921 ccgagggcgg catctcgtcg gaatccgaag cggtcgctgt cgccaatacg atcggctatc
     1981 cggtgctgat caaggcctcg gcaggcggcg gcggacgcgg catgaaggtg gcgcgttcgg
     2041 catccgaact gggactcgcc ctttcgaccg cgcgcgccga agccaaggcc gcgttcggcg
     2101 acgacgacgt ctatatggaa aaatatctgg ccaagccgcg gcacgtcgaa atccaagtca
     2161 tcgccgacag tcacggcaag gtgatccatc tgggcgaacg cgattgctcc ttgcaacgcc
     2221 gccatcagaa ggtgtgggag gaagcaccct cgccggcgct caatgtagaa ggacgaaatc
     2281 agatcggcgg cgtggtgacg gcggccctca ccaaactcgg ctatctcggc gtcggcacgg
     2341 tcgaattcct ttacgagaac ggccaattct atttcatcga gatgaacacg cgcctgcagg
     2401 tcgagcaccc ggtcaccgaa ctcgtcacgg ggctcgatct cgtgcgcgaa cagattcgca
     2461 tcgccgatgg cggcgcgctg gagatcgacc agagcgatgt cgtcgtcgcc ggtcacgcga
     2521 tcgagtgccg catcaatgcg gagaatccgt tcaccttcca gccgtcgccg gggcgcatca
     2581 cagatttcca cgcgcccggc ggattgggcg tgcgcatgga ttcggccgcc tatgccggct
     2641 acagcatccc gccctattac gacagcctga tcggcaagct gatcgtgcat ggcaaaagcc
     2701 gcaatgaatg cctgatgcgg ttgcgccgtg cgctggacga actggtgatc gggggaatcg
     2761 agaccacgat tccgctgttc caggaactga tcaaacaacc cgacatcctg gatggcgatt
     2821 acaatatcca ttggcttgag cattatctcg cccgccgggc gaccggtgcg ctgtagaaac
     2881 cgactttgca gcccgccgtc ctctatgatg tatggccaaa agaggcattc ccatgttccg
     2941 ttggctgaaa cgctggtcgc cgcaattgct gagtctcatg cgcatcgcgg tcggccttac
     3001 cttcctcgaa cacggcacac agaaattgct gaactttccc gtgccgcgtc cggggctcgc
     3061 attgccgctg cttctcttca ccggcatcct cgaaacggtt gcaggcgcgc tggtgacgct
     3121 cggactcatg acgcgcctag ccgcgtttct gctgtcgggc gaacttgccg tcggctattg
     3181 gtggatccat gagccgcgca gcccctatcc gatggccaat ggcggcgaag cgatggtgat
     3241 ttattgtttc gtgttcctct acctcgcggc ggccggagcc ggacccttga gcttggattc
     3301 gctgaccagg cgcgcatgat ttccgtgacc gcgatcaatc ccgaagaact gttgctcgcc
     3361 tatcgtctcg gcatatttcc catggccgaa agccgcacgg cgaaggacgt gctgtgggtc
     3421 aggccgcatg agcgcggcat cctgccgctc gacaaatttc acgtgcccaa gcgtctcgca
     3481 cgcgtggtgc gttccaaccg tttccacatc accgccgatg ccgccttcga ggatgtcatt
     3541 cgcgcatgtg ccgaatcgcg ccccgaacgc cgcgagacct ggatcaacga cgccatcctg
     3601 gacgtcttca tttcgcttca taaaagaggc ttcgcgcaca gcatcgaaac ctggcacggg
     3661 gataggcttg tcggcggact ttacggattg tcgctcagcg ccgcgttctt cgccgaaagc
     3721 aaattctcgc gggcgaccga tgcgagcaag gtcgccctcg tgcatctcgc ggcgcggctg
     3781 aaggccggcg gctacacgct gctggatgca caatttccca atccgcatct cgaacaattc
     3841 ggcgcggcca cggtgaccga agagcgcttc cagttcatgc tggcgcgcgc gctcgcggca
     3901 agcggcgatt tctacggact gggacgggcg ggcgttgcgc cggatggcgc ggcgggcgct
     3961 ccggtgacgg agggtggcga tggcggcgcg gtcccagccg ccggcgcgga ttcggtcgca
     4021 ggctcgtcgg tcttgcaggt gatcacccac acatcatagg tcggatgctc cagcgcactc
     4081 agcgccggcg tcgaagcgaa catccagccg gaaaacatcc gctttggagg cgcactcggc
     4141 ggattgtcgt ctatctggac gaagaccgtg gtttccggcg gttcttccgg cgggaccgta
     4201 taacaagagc gcacggtgag ggtgagcgtg ccgaaacgca ccggcacacc gacaggcgca
     4261 ttgatgtcga tggcgcggcc ggtgatcttg tcgaggccac ggaacacagc caggcgcctt
     4321 acgccatggg catcgggtat cgcatcggcg atggtggcag gcttgggtgg aagcggcatc
     4381 ggcgcggggg ccgcggccgc ggcattcaga gccagcacca atcccagcag cgtcaatgcg
     4441 gatgtgacaa cgtgaatctt cacttgccgc cgccaggcgt ggcgccggtg ctgaacacgg
     4501 ccttgccgag gaggccgata agatcgatcg atccctgcgt gttttcgatc tcgcctcccg
     4561 gtttgatggg ctgcatcgaa gcccccggct ctatggaaag atattggttg cccagaagtc
     4621 cctccgatgt gatgcgcacg gacgaatcgt cgggcagctt gatgctgttc tccagcgcga
     4681 gtgtcaccac cgcgttgtag gtcttcggat cgagtgccag cttttcaacc gtgcccacct
     4741 tgatgcccga caggcgcaca tcggtcccga cattcacgcc gtcggctttg gagaatttgg
     4801 cgatcacgtc atagcccgag accggccccg aaccggtgct cgaataggcg aacaccagga
     4861 acaacgcggc caccgcgacc actgcggcgc cgatcagggt ttcgacgatc gagttctgca
     4921 tgggatcatc cactctcatg gttcggcacg ctcttcatga ggaacgattc cccatcctga
     4981 gcctgtcgaa ggatcaaccg ggacgccagg cgtcataatc ccctgtcgca ggaggacgct
     5041 ggccgtcctt ccacaggctg ccgtccgggt gccaggcgcc tctcgtgccg gtcaggttcg
     5101 gctcatggtc gagttcccag ggcttgcgct tcggcggatc ttcggtcggc ggcagggcga
     5161 aagtgtgatg cagccagcca tgccagtccg gtggcactcg ggacgcctcg atggtgccgg
     5221 caaagatcat ccagcgccgc ttgccatcct tggtctggta gtagcgattg ccgaaatcgt
     5281 cgctgccgac cggaatgccg gagaatcggg tggtcagata ggtgcccggg gtggcgtttt
     5341 cccaccaccc gaaaagccga ctgaggaagg acattggcgg tttaatttcc gaatctacgc
     5401 cgggactata ggcgggcgcc cacgcccaca acaacggcgt gatgatggat gagcgtgtct
     5461 atttcgtgac gctttcgcgc ggattcgcac ggcatgacgc catcgcccgg agccaaattt
     5521 tttggccgtc ccatgcaccg acttttccac aggatatcaa caaggcaggc taagctactg
     5581 gatgtgaagt acaattttat ccaacacaga tatggccgtt gctcccattc cttagtcatt
     5641 atgctcttgc aatgtactaa atctggtata cgcttccctt ccttcgcggc gcttcgcagc
     5701 gaaggacctg atgcaaaaac cggccgttcg acggaaaaaa acgggggcta cccctgtgat
     5761 tttccgtaac cgcatcgctt tgtcatcggg gcgtcggaaa cgggttaagg ggggagcagg
     5821 ctatgcggat cacacgccat ttcacgaccg aaggaaaatc gccctatgag ggcatcgcct
     5881 tccggaccac cacaagcgaa atccgcaatc cggacggctc cgtcgtcttc cggcacgaag
     5941 gcatcgaggt gcctgaagcc tggagccagg tcgcctgcga tgtgatggcg cagaaatatt
     6001 ttcgccgcgc cggtctcgcg gtgtgcctga agcgggtcga ggaaaacaac gttcctccct
     6061 tcctctggcg tcacgtgcct catgaggagg cgctcgccgg actgcccgcc accgtcaaga
     6121 aggcgaccgg cgaagtcagc gccaagcagg tgttccatcg cttggccggg acctggacct
     6181 attggggctg gaaaagcggt tatttcgact ccgagaagga cgcacgcgcc ttttatgacg
     6241 agatgtgcca catgctggcg gcgcaaaagg ccgcgcccaa ctcgccgcaa tggttcaaca
     6301 ccggcctgca ttgggcctat ggcatcgatg ggccgagcca gggtcattat tacgtcgact
     6361 tccagaccgg cgaactggtg cgctcggcat cggcctatga gcatcctcag cctcatgcct
     6421 gctttatc
//
LOCUS       DASYEG010000294         8883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_548089, whole genome shotgun sequence.
ACCESSION   DASYEG010000294 DASYEG010000000
VERSION     DASYEG010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 8883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8883
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548089"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VGM72_12475"
     CDS             <1..382
                     /locus_tag="VGM72_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266127.1"
                     /translation="GVVPTTAKVGIDATIGEGIPKERYERIAYAYADTAKIGDYVKGK
                     SDASGKVGDERVVTQLADDILKRIKEKPLYYTQIAEAFGTYDFQTVARALGHLHTTER
                     LWQDAEGRCCERGSKFAAKPPGGS"
     gene            439..876
                     /gene="trxC"
                     /locus_tag="VGM72_12480"
     CDS             439..876
                     /gene="trxC"
                     /locus_tag="VGM72_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HEY4266128.1"
                     /translation="MNDQPRHIVCTTCGAVNRVPGAKNASAGKCGKCHQALFSGHPAA
                     ATTANFAKHIGKSDVPVVVDFWAEWCGPCKMMAPVYEKVAAELEPAFRFLKVDTEAET
                     TLAAQFGIRSIPTLMVFRDGAIVAQQSGAMDAGRLRDWLRQHA"
     gene            1090..1440
                     /locus_tag="VGM72_12485"
     CDS             1090..1440
                     /locus_tag="VGM72_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266129.1"
                     /translation="MKRFFALFQIPASVMEEWKKNTKPDEMKSASEKMMGEWQKWTKA
                     HEKNLLDRGAPLGKTKRVTANGISDMRNDLNWYSIVEAESHDAAAKLFSDNPHLQIPQ
                     AFIEVMEIPPLPQM"
     gene            1552..1947
                     /locus_tag="VGM72_12490"
     CDS             1552..1947
                     /locus_tag="VGM72_12490"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY4266130.1"
                     /translation="MNEEPEERAIREQIEAWAAAVRSRDMAGILKNHATDIVMYDVPP
                     PLQLRGIAAYRDSWPQFFDASPKPIRFDIMEMHITAGRDVAFATALMRCAIVEDGNAD
                     LDFRLTVGLRKIGGQWIVTHEHHSIPAVN"
     gene            1953..2453
                     /locus_tag="VGM72_12495"
     CDS             1953..2453
                     /locus_tag="VGM72_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY4266131.1"
                     /translation="MNPAIRRVDASALHQEKTAMPNTVRLHRVLATKPDKVYRAFTEA
                     DAMAKWLPPNGFTCTVHHLDAKVGGTHRMSFRNFTTGLSHSFGGKYLELVPGERLRYT
                     DKFEDPNLPGEMQVTVTLKQVLVGTELNIVQEGIPDAIPVEACYLGWQESLRNLATLV
                     EPEINQ"
     gene            complement(2492..3364)
                     /locus_tag="VGM72_12500"
     CDS             complement(2492..3364)
                     /locus_tag="VGM72_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266132.1"
                     /translation="MSRFVEQAWGPALLLSIAALAPSALADTPSKSAVPNFASVDFGW
                     QTNEEDWQPVAGSPDHGPIRNDPAYPFTNNASGNRTHTQVTVRVTNTKDPILKPWVAA
                     EIQKSNAEVLSGEHPIPFTAQSRCWPGGTPAQSLYPFEPVYFVQTQKEVWMIWQRDHV
                     VRRVFLEGRHPDHVKPSWFGDSIGHYENGDTLVIDTIGVAGGHNRFIDNFRTPHTDKM
                     HVVERFTIHPDGRGMTGTATVEDPDAFNGPITMKQEWFKADFPMLETVCPENNSDFFN
                     QNLFPIPQAGRPDF"
     gene            complement(3436..5244)
                     /locus_tag="VGM72_12505"
     CDS             complement(3436..5244)
                     /locus_tag="VGM72_12505"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEY4266133.1"
                     /translation="MPPLPFTTRPEIEGIFGVVASTHWIATAVAMSMLERGGNAFDAA
                     VAAAFTLQIVEPHLNGPGGDVPVILWDAKREKPIVICGQGTAPKAASIARFRGLGLDV
                     VPGSGLLAACVPGTFETWMMLLRDFGTMRVRDVLEPAIFYAREGHPLVDRETETIATV
                     EELFREHWKSSAAIFLPNGQVPPRGTLFKNPTLAATYERLLKEAESAGGDRDSQIERA
                     RETWSKGFIAETIGRFCATHEAMDTSGTPHGGLLTADDIAAWTPHTEAPVTYDYRGYT
                     VCKAGFWSQGPALLQQLALLKGFDLDGQDPAGPDFIHLQVECAKLAFADRDAFYGDPD
                     FARIPGDILLSDAYNDARRKLIGGRASLEQRPGIIPGFGGKVHIGEARGAVAGAGEPT
                     TGRMASAEPENGKLGEARGDTVHFDIIDRWGNMISSTPSGGWLQSSPVIPELGFCLGT
                     RMQMFWLDENHPASLAPGKRPRTTLSPTLALREGAPYLVWGSPGGDQQDQWICQFLLR
                     HIHAKHNLQQAIDAPAWHSEHFPTSFWPRVARPGVLVVEGRVPDNSVKELRRRGHIVE
                     VGPAWSEGRLTAASREGVRRRAAANPRGMQGYAAGR"
     gene            complement(5282..6184)
                     /locus_tag="VGM72_12510"
     CDS             complement(5282..6184)
                     /locus_tag="VGM72_12510"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4266134.1"
                     /translation="MEANRQLPLGAEIFLDHIAHFVRHREAASKALARAGFAPTPISI
                     QTDKGQLTGTGNVTAMLTRGYIEVLFKTADTPLGQELDAALARYSGVHLMAFGVAHAV
                     AESARLERAGFHIRPIVELRRPVATQTGHAQAAFTVARVDAGDMAEGRIQMLTHHTED
                     AVWQKRWLTHSNGARQLIDVVAAVASVEEAAARYARFLGRKAAATRTGWGVTLDRGGV
                     QLMDANAFSRLVPGVPVPSLPFIGAYAIGVESRNDTAAILRANGMALVERDGMLLVPF
                     PPELGQGAWIFVERSTDLPWRANS"
     gene            complement(6185..6997)
                     /locus_tag="VGM72_12515"
     CDS             complement(6185..6997)
                     /locus_tag="VGM72_12515"
                     /inference="COORDINATES: protein motif:HMM:NF009825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HEY4266135.1"
                     /translation="MSATIRVGIAGLGAIGRTVASRLHQNAIPKMNLAGVAVRDPAKA
                     QSFLASIGCDAPILSLAQLAGTCDLVIECAPAAAMAEIAAAVLGAGKSIMVLSAGMLL
                     RHPELLAAAKEHGGQIIVPTGALLGFDAVSAAAEGEIQSVTMITRKPVNGLMGAPYLA
                     KNNISIDGLAQAKRVFKGSAREAAIGFPENVNVAAALSLAGVGPDRTSIEIWADPQVH
                     RNMHRVVVEADSARFEMAIENVPSENPKTGRITALSVLAALRKMTAPLRVGT"
     gene            complement(6994..7836)
                     /locus_tag="VGM72_12520"
     CDS             complement(6994..7836)
                     /locus_tag="VGM72_12520"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY4266136.1"
                     /translation="MTNMPNVKFPTGETVPALGQGTWMMGDSPSRRKTEADALRLGID
                     LGMTLIDTAEMYASGGAEEIVGEAIAGRRDEVFIVSKVMPDNASRRGTVAACEKSLRR
                     LKTDRIDLYLLHWPGPAPIAETVDAFTTLKHAGKIRAWGVSNFDVDEMEDVAALADGG
                     AVATNQVMYNLKRRGIEYDLIPWCAQRGIPIMAYSPLDQGRLLRSRELEAIAARHKAS
                     VAQVALAWALQQRQVMVIPKAGSVAHVRENYGALAVALDARDMAALDRAFPPPAKKKP
                     LEST"
     gene            7900..8739
                     /locus_tag="VGM72_12525"
     CDS             7900..8739
                     /locus_tag="VGM72_12525"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4266137.1"
                     /translation="MWILRFLTALLGLFGEGRSPAMMLNLVVPRSGYRLRRDLPYGPG
                     PRQRFDLYLPETLPSPAPVLLFFYGGAFRTGRKSEYRVVGEAFASAGIIVAVADYRIY
                     PNARFPGFLEDGAMALAAVHARAAEFGGDPNRIFLAGHSAGAYIAVMLAADPSYVRGA
                     RADPAWIRGVVGIASSYGNSPPGVVPNPVFEGRAPAQTQPGFFIDAKRPPMLLVSGAK
                     DIAANLESARNLAAKLRAVASEVEEIVYPNPGHMGIILSLAPRFRHIAPLRDDIARFM
                     AAH"
BASE COUNT         1692 a         2753 c         2781 g         1657 t
ORIGIN      
        1 cggcgtggtg ccgacgacgg ccaaggtcgg catcgatgcc accatcggcg aaggcatccc
       61 gaaggaacgc tatgagcgga tcgcctatgc ctatgccgac accgcgaaga ttggcgatta
      121 tgtgaagggc aagagcgatg cctcgggcaa agtgggcgat gaacgcgtcg tgacccaatt
      181 ggcggatgac atcctgaaac gcatcaaaga gaaaccgctc tattacacgc aaatcgcgga
      241 agccttcggg acgtatgatt ttcagacggt cgcgcgcgca ctaggccatc tgcacacgac
      301 cgagcggtta tggcaggatg ccgaaggacg ttgttgcgaa cgcggctcca aattcgccgc
      361 gaaacctccg ggggggtctt gaactcacgg caaacgctta cgaagttcac ctgagtcaat
      421 caggctgccg ggtatacgat gaacgaccag ccgcgccaca tagtctgcac aacctgcggc
      481 gcggtaaacc gcgttccggg cgccaagaac gcctcggcgg ggaaatgtgg aaaatgtcat
      541 caggcgcttt tcagcggaca tcccgccgcg gcgaccaccg cgaattttgc aaagcacatc
      601 ggcaaaagcg acgtgcctgt ggtggtcgat ttttgggcgg aatggtgcgg gccctgcaaa
      661 atgatggcgc cggtctacga gaaagtcgct gccgagctgg aaccggcgtt tcgtttcctt
      721 aaagtggata cggaagcgga aaccacgctg gctgcccaat tcggcatccg cagcattccc
      781 acgctgatgg tcttccggga cggtgcgatc gtcgcgcaac aatccggtgc catggatgcc
      841 ggccgtctgc gcgattggct ccgccaacac gcttaatttc agcctctcct tgaggagggg
      901 ggacatgtcc gcttatgacc ccaaagtgaa ccgggggtgg cggcttgcag accctttcca
      961 aggtgaccaa gcggacggtc ccgcgccttg gcccaaaaaa tctcacaagc gggtgtcgat
     1021 cccacgggtc gccgttcgtc gtatcaccga ggggtaggcc ccgttacgca ccagaacagg
     1081 agatcagcca tgaaacggtt ctttgctttg tttcagatac ctgcgtcggt catggaagag
     1141 tggaagaaga ataccaagcc ggacgagatg aaatcagcgt ccgagaaaat gatgggtgag
     1201 tggcaaaaat ggacaaaggc gcacgaaaag aacctcctcg atagaggggc gcctctcgga
     1261 aaaaccaagc gtgtcaccgc caacggtatc tccgacatgc ggaacgattt gaactggtat
     1321 tcgatcgtag aagcggaatc tcatgatgcg gcggcgaaat tattttccga taacccgcat
     1381 ctgcagattc cacaggcgtt catcgaagtc atggagattc cccctcttcc tcagatgtga
     1441 tgtctgtcgc aacgccgcaa cggcgactgt gagcgcatac atggtcttac ggccattcac
     1501 agtcgcgcgc gtggccaatg cggttccacc aatgataacc ggacgactac aatgaatgaa
     1561 gaacctgaag aacgcgccat tcgtgagcaa atcgaagcat gggccgcggc cgttcgcagc
     1621 cgggacatgg cgggcattct taaaaaccat gcgacggata tcgtgatgta cgacgtgccg
     1681 ccgccgttgc aattgcgcgg cattgccgcc tatcgggata gctggccgca attctttgac
     1741 gcctcgccca agccgatccg ttttgacatc atggaaatgc acatcacagc cggccgggac
     1801 gttgcttttg cgacggccct catgcgctgt gcaattgttg aagacggcaa tgccgacctc
     1861 gatttccgct tgaccgttgg gttgcgcaag atcggcggtc agtggattgt cacccacgaa
     1921 catcactcca ttccagccgt gaattaagcc cattgaaccc cgcgatccgt cgcgtcgacg
     1981 cgagcgcctt gcatcaggag aaaacagcca tgcccaatac cgttcgattg caccgcgtcc
     2041 tggccacgaa acccgataag gtctatcgcg ccttcaccga ggcggacgcc atggcgaagt
     2101 ggctgccacc caacggcttt acctgcaccg tgcatcactt agacgcgaaa gtcggcggca
     2161 cgcacaggat gtcgttccgc aatttcacca cgggcctcag ccattcgttt ggtggaaaat
     2221 atctggaact cgtgcccggc gagcgcctgc gctacacgga taaattcgag gatcccaatt
     2281 tgccgggtga aatgcaggtg acggtgacgc tcaagcaagt attggtcggc accgagttga
     2341 acatcgtgca ggaaggcatt cccgacgcca ttccggtaga agcgtgctac ctcggctggc
     2401 aggagtcgct gcgaaatctc gccaccctcg tcgagccgga aatcaatcag tagcaaaaag
     2461 gacagtgcat ctgccatgtc cgctcatggc gtcaaaaatc cggtctgccg gcttgcggaa
     2521 tgggaaacag attctgattg aagaaatcgg aattgttttc cgggcagacc gtctccagca
     2581 tcgggaagtc cgccttgaac cattcctgtt tcatggtgat cgggccgttg aaggcatcgg
     2641 ggtcttccac ggtcgcggtg cccgtcatcc cgcggccgtc gggatggatg gtgaagcgtt
     2701 cgaccacatg catcttgtcg gtgtggggtg tgcggaaatt gtcgatgaag cggttatggc
     2761 cgcccgccac cccgatggtg tcgatcacca gcgtatcgcc gttctcataa tgcccgatgg
     2821 aatcgccgaa ccatgagggc ttcacatggt cggggtgacg gccctccagg aacacccgcc
     2881 gcacgacatg gtcgcgctgc cagatcatcc agacctcttt ctgcgtctgc acgaaataaa
     2941 ccggctcgaa cggataaagc gattgcgccg gtgtgccgcc cggccagcag cgcgattgcg
     3001 cggtaaacgg aatagggtgc tcgccgctga ggacttccgc attgctcttc tgaatctcgg
     3061 ctgccaccca tggcttcagg atggggtcct tggtgttggt gacgcgcacg gtgacttgcg
     3121 tatgggtgcg gttgccgctg gcattattgg tgaagggata ggcgggatcg ttgcggatcg
     3181 gcccatgatc ggggctgccg gcaacgggct gccaatcttc ttcattggtc tgccagccga
     3241 aatcgaccga tgcgaaattg ggaacggcgc tcttggacgg cgtgtccgcc agcgccgagg
     3301 gtgcgagggc ggcaatcgaa aggagaagcg cagggcccca agcctgttcc acgaagcgcg
     3361 acatgtgtcc tccacgggca gaaggctttt ctttcacttt agcgtccgca agcggaggag
     3421 gaaaggcgcg ttcctctaac gcccggcggc atagccctgc atgccgcgcg gattggcggc
     3481 ggcacggcgg cgcacgcctt cgcgcgaggc ggcggtaagg cgcccctcgg accaggccgg
     3541 gccgacctcc acgatatgtc cccgccggcg caattccttc accgaattgt ccggcacgcg
     3601 cccttcgacc acgaggacgc cgggacgtgc gacgcgcggc cagaacgagg tggggaaatg
     3661 ctccgagtgc caggccgggg catcgatggc ctgctgcaga ttgtgcttgg cgtggatgtg
     3721 ccggagcagg aattggcaaa tccactgatc ctgctgatcc ccgcccggcg aaccccagac
     3781 gagatagggc gcgccttcgc gcagcgcgag cgtcggcgat agcgtggtgc gcgggcgttt
     3841 tcccggcgca aggctggcgg gatgattctc atccagccag aacatctgca tgcgcgtacc
     3901 gaggcagaat cccaattccg ggatcaccgg cgaggattgc agccagccgc ccgacggcgt
     3961 tgaggaaatc atgttgcccc agcggtcgat gatatcgaaa tgcaccgtgt cgccgcgcgc
     4021 ttcccccaat tttccgtttt ccggttcggc gctcgccatg cgccccgtgg tgggttcgcc
     4081 ggcgcccgcg accgctccgc gcgcttcgcc gatgtgaacc ttgcccccga agcccggaat
     4141 gattccgggg cgctgttcca ggctggcgcg tcccccgatc agtttgcggc gcgcgtcatt
     4201 gtaggcatcg gacaacagga tatcgccggg gatgcgggcg aaatccggat cgccataaaa
     4261 cgcatcgcgg tcggcgaaag cgagcttggc gcattcgacc tgaagatgga tgaaatccgg
     4321 ccccgccggg tcctgcccgt cgagatcgaa ccccttcagc agcgcgagct gttgcaggag
     4381 cgcaggtccc tggctccaga acccggcctt gcagacggtg tagccgcgat aatcataggt
     4441 gacgggcgct tcggtatggg gtgtccatgc cgcgatatcg tccgccgtca acagaccgcc
     4501 atgaggggtg ccgctggtgt ccatcgcttc atgggtggcg cagaaacggc cgatggtctc
     4561 cgcgatgaaa cccttcgacc aggtttcgcg cgcgcgctcg atctgggaat cgcggtcgcc
     4621 gccggcactt tcggcttcct tcagcaaccg ctcataggtg gcggccagcg ttgggttctt
     4681 gaacagcgtt ccgcgcggcg gcacctgacc gttgggaagg aaaatcgcgg ccgaactttt
     4741 ccaatgctcg cgaaacagct cttccacggt ggcgatggtt tcggtttcgc ggtccaccag
     4801 cggatggccc tcgcgggcat agaagatcgc gggctccagc acgtcgcgca cgcgcatggt
     4861 gccgaaatcg cgcagcagca tcatccaggt ttcgaaagtg cccggcacgc acgcggccaa
     4921 caggcccgat cccggcacca catcgagacc gagcccacgg aaccgcgcga tggaggcggc
     4981 cttgggcgcc gtgccctgac cgcagatcac gatgggcttt tcgcgcttgg catcccacag
     5041 gatcaccggc acatcgccgc caggtccgtt caaatgcggt tcgacgatct gcagcgtgaa
     5101 ggcggcggcg acggcggcgt cgaaggcatt gccgccgcgc tccagcatcg acatggcgac
     5161 tgcggtggcg atccaatggg tggaggcgac gacaccgaaa atgccttcga tttcgggacg
     5221 cgtggtgaag ggaagtggcg gcatgggtga accgtggatg gggggcgcgg ggccgcacca
     5281 ttcacgagtt cgcccgccac ggcaagtccg tcgaacgctc cacgaagatc cacgcgcctt
     5341 gtccgagttc gggcggaaac ggcaccaaca gcatgccatc gcgctcaacc aaagccatgc
     5401 cattcgcgcg caaaatcgcc gccgtatcgt tccgggactc cacgccgatc gcataggcgc
     5461 cgatgaaggg caggcttgga accggcacgc cgggcaccag acgcgaaaac gcatttgcgt
     5521 ccatgagctg gacaccgccg cggtcgaggg tcacgcccca ccccgtccgc gtggccgccg
     5581 ccttgcggcc cagaaaacgc gcataacggg cggcggcttc ctccacgctc gcgacggccg
     5641 cgacgacatc gatcaattgc cgcgcgccgt tggagtgggt gagccagcgc ttttgccaca
     5701 ccgcgtcttc ggtatggtgc gtcagcatct ggatgcgacc ttcggccatg tcgccggcat
     5761 cgacgcgcgc caccgtgaag gcggcttgcg catgtcccgt ttgcgtcgca accggccggc
     5821 gcaattccac gatggggcgg atatgaaacc ctgcgcgttc cagccgcgcg ctctcagcca
     5881 ccgcatgggc gacgccgaac gccatcagat gaacgccgga atagcgcgcg agtgcggcgt
     5941 cgagttcctg accgagcggc gtatcggcgg tcttgaacag cacctcgata tagccgcgcg
     6001 tcagcatcgc ggtcacattg ccggtgccgg tcaattgccc cttgtccgtc tggatggaga
     6061 tgggtgtggg cgcaaaaccg gcacgcgcga gcgctttgct cgccgcctcg cggtgacgca
     6121 cgaaatgcgc gatatggtcg aggaaaatct cggcccccag cggcaattgg cgattcgcct
     6181 ccattcaggt gcccacccgc aagggcgcgg tcatcttgcg caacgcggcg agaacggaca
     6241 gcgcggtgat gcgcccggtc ttcgggttct cgctcggcac attctcgatg gccatttcga
     6301 aacgcgcgga atcggcttcg accacgaccc ggtgcatgtt gcgatgaacc tgcggatcgg
     6361 cccagatctc gatgctggtg cggtccggac ccacgccagc gagcgaaagg gcggccgcca
     6421 cattgacgtt ttcgggaaaa ccgatggccg cctcccgcgc gcttcccttg aacacgcgct
     6481 tggcttgcgc cagcccgtcg atggagatgt tgttcttcgc cagatagggc gcgcccatca
     6541 acccgttcac gggcttcctc gtgatcatgg tcaccgactg gatctcgcct tccgccgcgg
     6601 cggagaccgc atcgaagccc agaagcgcac ccgtcggcac gatgatctgg ccaccgtgct
     6661 ccttggcggc ggcaagcaat tcgggatggc gcagcagcat gccggcgctc agcaccatga
     6721 tggatttgcc tgcgccgagc accgctgcgg caatttcggc catcgcggcg gcaggcgcgc
     6781 attcgatgac gagatcgcaa gtccccgcca attgcgccag cgagagaatg ggcgcgtcgc
     6841 agccgatgct cgccagaaag ctctgcgcct tggcgggatc gcgcaccgcg acaccggcga
     6901 gattcatttt cgggatggcg ttttggtgca gccgcgacgc caccgtgcgg cctatcgcgc
     6961 cgagtccggc aatgcctacg cggatcgtgg cgctcatgtc gattccaatg gcttcttttt
     7021 cgcagggggc ggaaaggcgc gatcgagtgc ggccatgtcc ctcgcgtcca gcgcgactgc
     7081 aagcgcgcca taattttccc gaacatgcgc cacactgccc gccttgggga tcaccatcac
     7141 ctgtcgctgt tgcagggccc aggcgagcgc cacctgtgcc acgctcgcct tgtgacgcgc
     7201 cgcaatcgcc tccaactcgc gtgatcgcaa gagccggccc tgatccaacg gcgaataggc
     7261 catgatggga atgccgcgtt gggcacacca gggaatgaga tcatattcga tgccgcgccg
     7321 cttcagattg tacatcacct gattggtcgc caccgcgcct ccatcggcaa gcgccgccac
     7381 gtcttccatc tcgtccacat cgaaattgct gacaccccaa gcgcggatct tgccggcgtg
     7441 cttcagcgtg gtgaaggcat cgaccgtctc cgcgatgggc gcaggcccag gccaatgcag
     7501 cagatagaga tcgatgcggt ccgttttcag ccgcctgaga ctcttctcgc aggcagcgac
     7561 agtgccgcgg cgcgatgcgt tgtccggcat caccttgctg acgatgaaca cttcgtcgcg
     7621 ccggccggcg atggcttcgc ccacgatttc ctccgcgccg ccgctggcat acatttcggc
     7681 ggtgtcgatc agcgtcatgc cgagatcgat gccgagccgg agcgcgtcgg cttcggtttt
     7741 gcgccggctg gggctgtccc ccatcatcca ggtgccctgg ccgagcgcgg gcacggtttc
     7801 gccggtggga aatttcacat ttggcatgtt cgtcatggct ggaggatagc accgcccggc
     7861 attttggaaa tcaccgaaaa aaccggataa aagtgttgca tgtggatttt gcgattcctc
     7921 accgcgctgc tgggtctgtt tggggaaggg cgttcgcccg ccatgatgct caatctggtg
     7981 gtgccgcgct cgggctatcg cctgcggcgc gatctgccct atggcccggg gccgcgccag
     8041 cgtttcgatc tgtatctgcc cgagacactg ccttcgcccg cgccggtgct gctgttcttt
     8101 tatgggggcg cgttccggac gggccggaaa agcgaatacc gcgtggtggg cgaggcattc
     8161 gcgagcgcgg gcatcatcgt ggccgtcgcg gattaccgga tttatccgaa cgcacggttc
     8221 cccgggtttc tggaggacgg cgcgatggcg ctggccgcgg ttcatgcgcg cgccgcggaa
     8281 ttcggcggcg atcccaaccg catcttcctc gccggtcatt cggcgggtgc ctatatcgcg
     8341 gtgatgctgg cggccgaccc cagctatgtc cgcggcgcgc gcgccgatcc cgcatggata
     8401 cgcggcgtgg tcggcatcgc gagttcctat ggcaattcgc cgcccggagt ggtgccaaac
     8461 cctgtcttcg aggggcgcgc gccggcgcaa acccagccgg gttttttcat cgacgcgaaa
     8521 cgcccgccga tgcttctggt cagcggggcg aaagacattg ccgccaacct tgagagcgcc
     8581 cgcaatctgg ccgcgaaact gcgggctgtt gcaagtgagg tcgaagagat cgtctatccc
     8641 aaccccggac acatggggat catcctgtcg ctcgcgccgc gatttcggca catcgcgcct
     8701 ttgcgtgacg atatcgcgcg cttcatggcc gcgcattgat ggcaacatcc ttcgagagac
     8761 ccttttcggt tttggttcga caccgtaaag tcacatatgc aagggcacat ggatggagtt
     8821 ccccgcctgg aatttgaaat gattttcatt ttccgggaca ttgcaaacga aggtttggtt
     8881 aga
//
LOCUS       DASYEG010000295         8342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3881526, whole genome shotgun sequence.
ACCESSION   DASYEG010000295 DASYEG010000000
VERSION     DASYEG010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8342
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3881526"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VGM72_12530"
     CDS             complement(<1..375)
                     /locus_tag="VGM72_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266138.1"
                     /translation="MKLRFVSLNHFKNILNSGQVAVQPDVTCVVGKNESGKTAFLQCL
                     HRLNPAQANASFNAQRQYPAWLEKQHRRQKDLSTVEPITAIFELTDSEWSEIADKFGK
                     SALSRREVVIARSYGDTLRFEFA"
     gene            complement(477..1322)
                     /locus_tag="VGM72_12535"
     CDS             complement(477..1322)
                     /locus_tag="VGM72_12535"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY4266139.1"
                     /translation="MPKRLTAEQVESYGRDGFVSPVRVLTPAAAENCRRELEAMETAS
                     GGALRGLNRTKFYLRHPWAHRLATDPAILDGVEDLIGPDILLYQNTVWAKNAGEDSRV
                     SWHQDNTYFGHVPCEVLSVWIALSPSRPESGSMRFLPGSHKFGQLPVRHEVQDGNMLS
                     SGQVTDIDVSGFEPFATSLAPGEASIHHAFLIHGSPPNATGQRRLGITFVYHSPPLRQ
                     LGTVRTSALLVRGEDRYGNFEPEQPPLSPDDPGTIARHERGAALYRAKAEELGNATIA
                     RHDST"
     gene            complement(1361..2275)
                     /gene="bla"
                     /locus_tag="VGM72_12540"
     CDS             complement(1361..2275)
                     /gene="bla"
                     /locus_tag="VGM72_12540"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B3 metallo-beta-lactamase"
                     /protein_id="HEY4266140.1"
                     /translation="MKATTLLNMFGCVAGAMALAGANAAQAQNPPPPDHPTVTVHGKT
                     YTPRTVLSRNMGTREDQETQFPPHKIIGNIYYVGTKTLSSFLIVTPQGNILMDSTYER
                     NVPGIAKSVADLGFKFSDIKILLGNHAHADHQEGDAEVKQMTGAQVIAMAEDVPALKA
                     MKPGGKEHPVDKIIHDGDTVSLGGVTLTAHLTAGHTRGCTSWTTTASEGGKTYNVLLG
                     CSLRTPAVLTPSLIDEFNRTFKVVRTLPCDVQLGDHGAQYGMQEKYAKLKNGGPNPFI
                     EPATCMQEADLDEAMFHAQLDEQKAAHP"
     gene            complement(2338..3438)
                     /locus_tag="VGM72_12545"
     CDS             complement(2338..3438)
                     /locus_tag="VGM72_12545"
                     /inference="COORDINATES: protein motif:HMM:NF022591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HEY4266141.1"
                     /translation="MRALAHPLVALPLSAFRRYERHLSAVSMVAGFAFDNYYLDRVDH
                     PIAQLALFGYVLSAIASIVLVHLIETIEPEGLLLKARPLLVVATQFSFGGLWSAFLIF
                     YGRSAVIAASWPFLIVLAAMLIGNEVFAKYQSRLAFTSTLLFFALFSYAIFVVPLFTR
                     ALGQRTFLLSGGVAVACFGAVLTMLSAIGPERMRAAWRGIALGALGVFAILNLFYFTN
                     ILPPLPLTLTKAGVFHSVTKTGDVYQAMAEPATFLGAAGAAPVLHVAPGEPLSVYSAV
                     FAPIQLRTNIVHLWQHYDTARARWQTEAAVSFPITGGRDGGYRGSSTKSSPRSGQWRV
                     EIATADGRLIGRVPFSVVPVPATPATVLQILK"
     gene            3603..4943
                     /locus_tag="VGM72_12550"
     CDS             3603..4943
                     /locus_tag="VGM72_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266142.1"
                     /translation="MKRTHHHLAAAALALAGVAAGAPPALAQSHPEYIPLPRVQAALY
                     KPDSGPAPHVAFLVAHRTANNLSTNACAELAQRGFMVLCFNTRFVNNEAQVRWEETPL
                     DVKAAVEFVRRQPGITKVVLLGHSGGSPLMSLYEAIAENGAAYCQRPERIVKCGNDVD
                     GLKPVDAIVFPDAHPGNPVQALRDLNPSVVMGEDGKLKVIPELDPFSPANGFNPNGVS
                     HYSKEFQTRYYAAQSKAMTDRLARVQADEARIRRGDYPYPDDGLVMIPGGGNPGAGAG
                     GDGVLIAMDPDIPELMSTARPEKLLKNDGTIVTQIVHSVAVPEPRNAKTNRAFHTGTK
                     IFTFKSFLSANATRSTNSLDGIEDCSSNNSTVCAVQSIHVPILVAAMGGYHFVRDQEV
                     IYDKSASTDKDYVVIEGALHGYTPCTACEKTPGQYSNTMKNLFDYIAAWTNKRF"
     gene            4957..6033
                     /locus_tag="VGM72_12555"
     CDS             4957..6033
                     /locus_tag="VGM72_12555"
                     /inference="COORDINATES: protein motif:HMM:NF005071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY4266143.1"
                     /translation="MMKTLGLIATALSFTASAAFAQTTWPNQKDAFFTIKDFHFANGE
                     TLPELRLHYVTFGTPKKNAAGEIINGVILLHGTSGAGKSWFLPSLADELYKPGQPLSA
                     EDYFIIVPDAIGVGGSSKPSDGLKGKFPHYRYHDTVEAEHRLITEALGIKHLRLVLGS
                     SMGAMQTWMWGEMYPDLMDGLVPLASQPIGISGRNWINRRIRIEAIKHDPDYNNGMYD
                     KPPTHWIWTAASGPMGTESDYQIQQIAPTREAGDAFYKKLVEDASKADANDTLYGIEA
                     VEDYNPTPDLHKIKAKLMAINSADDEANPAALHVMEPAIAKIPGARYVLIPQSPETHG
                     HYTHLRAAIWKPYLVEFLKTLPPM"
     gene            6093..6854
                     /locus_tag="VGM72_12560"
     CDS             6093..6854
                     /locus_tag="VGM72_12560"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY4266144.1"
                     /translation="MKAWSKFIAVTMAGCLSVLIAGVPARAGEISVLAGGGPMPDVMG
                     TLIPMFEKATGNKVKIAFKGNPAIGMDLKQGAADLVITNSEFVDELATGGDLAANGKT
                     LLMISKVAVAVKKGAPRPDVSTPDKLKAALLAAKTVGYSQGSSGVHFLTVIQKLGIAD
                     AVKAKAVIVQGRPVGAAIAAGEAEIGVQQVAELRPVAGVDVIGELPPALQKQIPYSAG
                     VAAKAKDAATARALVAFLRTQPAMDVLKRKGMDLP"
     gene            7081..7671
                     /locus_tag="VGM72_12565"
     CDS             7081..7671
                     /locus_tag="VGM72_12565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY4266145.1"
                     /translation="MPDNILVFYGSYRATRIGIRMASFVINELRARGAVPELIDAREI
                     GLPIIDRMYKEYPPGSAPKAMEDLARKIRAADAFVFVVGEYNWGPQPGLKNLTDHFLE
                     EWFWRPAAIVSYSPSRLSGVRASSAWHTILSEMGMVVVSSTVAVGPIAETLDAQGEPI
                     GEVGRLAQKALHRLADDLAWWTSAARNERLQRAPPY"
     gene            complement(7725..8180)
                     /locus_tag="VGM72_12570"
     CDS             complement(7725..8180)
                     /locus_tag="VGM72_12570"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4266146.1"
                     /translation="MSNVQIEQKTYEMPPRDGFTVAHFLTVADIDRSAHFYETVFGGR
                     ILSRGDSSGAPGYIQIANTWLIVNVGGGPTPDKPSVTLSVLADPDKISSFMNIRVADI
                     RACYELWKSRGAQFITEPKDKYGETRCYIRDPDGYIIEIGQSKPDFTYG"
BASE COUNT         1722 a         2534 c         2535 g         1551 t
ORIGIN      
        1 agcaaattca aatctcaacg tatctccata agaccgcgca atgacaactt ctcgcctgga
       61 caaagcactt ttgccgaatt tgtcagcgat ttcgctccac tcggagtcgg tcaattcgaa
      121 gatagccgtt atgggctcga cggtgctcaa gtctttctgg cgtcgatgtt gcttttctaa
      181 ccaagcgggg tattgacgtt gagcattgaa tgaggcgtta gcctgcgctg gattcaatcg
      241 atgcaggcac tgcaagaagg cagtcttgcc agactcgttc tttccaacga cgcatgtgac
      301 gtcaggctgg accgccacct gacccgagtt gaggatgttc ttaaaatgat tgagcgatac
      361 aaatcgaagt ttcatgggaa ctcccgaagg gccaaaattt tgctcttcag tcccgaatat
      421 catgtttaga acaaatagtg aactatgtat tccgcggtgg agccctctat gcctgcctat
      481 gttgaatcgt gccgcgcaat cgtggcattt cccagttctt cagccttggc ccggtacagc
      541 gcggcgccgc gttcatgccg ggcgatggtg ccgggatcgt ccggcgatag cggcggttgc
      601 tcgggctcga aatttccata acggtcctca ccacgcacca gaagcgcgct ggtgcgcacc
      661 gtgccgagct gccggagcgg tggggaatgg tacacgaagg tgatgcccag tcggcgttgc
      721 ccggtggcat tcggcggcga accatggatg agaaaggcgt ggtgaatgga tgcttcgccg
      781 ggtgcgagcg aagtcgcgaa cggctcgaat cccgatacat cgatatcggt gacctgcccg
      841 ctcgacagca tgttgccgtc ctgcacctca tgtcggacgg gcaattggcc gaatttgtga
      901 gagccgggca gaaagcgcat cgaaccgctt tcgggccgtg acggcgacag cgcgatccac
      961 acgcttaaga cctcgcacgg gacatgcccg aaataggtat tgtcctgatg ccagctaacg
     1021 cgggaatcct ctccggcatt cttcgcccac accgtattct gatagagcag gatatcgggg
     1081 ccgatcagat cctccacgcc gtcgagaatc gccgggtccg tggccagccg gtgcgcccag
     1141 ggatgacgca gatagaattt ggtgcggtta agaccgcgaa gcgcgccgcc ggacgccgtc
     1201 tccatcgctt cgagttcgcg ccggcaattt tccgcagcgg ctggcgtcag aacacgcacc
     1261 ggcgagacga aaccgtcgcg cccgtagctt tccacctgct ctgccgtcag acgcttgggc
     1321 acggccggct attgcgcccc cgccgcgatc cgcacgcggt tcacggatgg gcggccttct
     1381 gttcgtcgag ctgcgcgtgg aacatggcct cgtccagatc cgcttcctgc atgcaggtcg
     1441 ccggttcgat gaaaggattg gggccgccat tcttcagctt ggcgtatttc tcctgcatgc
     1501 cgtattgcgc gccgtgatcg ccgagctgca catcgcacgg gagcgtgcgc accactttga
     1561 aggtgcggtt gaactcatcg ataagcgacg gcgtcagcac cgcaggcgta cgcaggctac
     1621 aacccaaaag cacattatag gtctttccgc cctcgctcgc cgtggtggtc caactggtac
     1681 agccgcgcgt gtggcccgcc gtcagatgcg ccgtcagcgt cacgccgccg aggctcaccg
     1741 tgtcgccgtc atggatgatc ttgtcgaccg ggtgctcctt gccgccaggc ttcatcgcct
     1801 tcagtgccgg cacgtcttcg gccatcgcga tgacctgcgc acccgtcatc tgcttgacct
     1861 ctgcatcgcc ctcctgatga tcggcgtggg cgtggttacc gagcaatatc ttgatgtcgg
     1921 aaaatttgaa tccgagatcg gcaaccgatt tcgcgattcc cggcacgttg cgctcatagg
     1981 tgctgtccat caggatattt ccctgcggcg tcacgatgag gaacgagctg agcgtcttgg
     2041 ttccgacata atagatgttg ccgatgatct tgtgcggcgg gaattgcgtt tcctgatcct
     2101 ctcgcgtgcc catgttgcgc gaaagaaccg tgcggggcgt gtaggtcttg ccgtgaacgg
     2161 tcaccgtcgg atggtcgggc ggcggcggat tctgcgcctg ggccgcattg gcgccggcaa
     2221 gcgccatcgc gccagctacg catccgaaca tattcaaaag ggttgtggct ttcatgcggt
     2281 cctccagcag ctcgcatgag cgtaacaccg gcgcttttgc gacactaggg agcgaatcta
     2341 cttcaggatt tgcagcacag tcgcgggcgt ggccggcacg ggcaccacgg aaaacggcac
     2401 gcgcccgatc aggcggccat cggcggtggc gatctccaca cgccattgac ccgagcgcgg
     2461 gctggatttg gtggaagagc cgcgatagcc gccatcgcgc ccgccggtga tgggaaagct
     2521 caccgcggct tcggtctgcc agcgcgcgcg ggcggtatcg taatgctgcc agaggtgcac
     2581 gatattggtg cgcaactgga tcggcgcaaa aaccgcgctg tagaccgaca gcggctcgcc
     2641 gggcgcgaca tgcaggacgg gggcggcacc ggccgcaccg aggaacgtgg cgggttcggc
     2701 catcgcctga tagacgtcgc cagtcttggt caccgaatga aacacgccgg ctttggtgag
     2761 cgtcagcggc agtggcggca agatgttcgt gaaatagaac agattgagga tcgcgaaaac
     2821 gcccaacgcg cccagcgcga tgccgcgcca ggccgcgcgc atgcgctccg gcccgatggc
     2881 cgacaacatt gtgagaaccg cgccgaaaca ggcgaccgca acgccgccgc ttagcaagaa
     2941 cgtgcgctgg ccgagcgcgc gcgtgaacaa gggaacgacg aagatcgcat aggagaagag
     3001 cgcgaagaac agcagcgtcg aggtgaaggc caaacgagac tggtatttcg cgaagacctc
     3061 gttgccgatc agcatggcgg ccagcacgat gagaaacggc caggacgcgg cgatcacggc
     3121 acttcggcca tagaagatca gaaaagcgct ccacaggccg ccaaacgaaa attgcgtggc
     3181 caccacgagc agcggacggg ctttcagcaa aaggccctcg ggttcaatcg tctcgatcag
     3241 atgcaccagg acgatggacg caatggcgga cagcacatag ccgaacaggg ccaattgcgc
     3301 gatcggatga tccacccggt ccagataata attgtcgaac gcgaagcccg cgaccatcga
     3361 gaccgcggac agatggcgtt catagcgccg gaatgcggaa agcggcagcg ctaccaaggg
     3421 atgtgcaagg gcacgcaaag ctaccgcctc attgaccggc ccgattggtg cgagaagact
     3481 atgtccgctt cgaggcagac actcgcatgg aaagcggctt ttcgcgcttt ggaaaacaca
     3541 atactctcct tgtcgatcaa ggacgatcca gaccggcctt ggacggcgca agggaggaaa
     3601 ccatgaaacg cacgcatcat catcttgccg cggcggccct tgcgctcgca ggcgtcgcag
     3661 ccggcgcacc gccggctctg gcacagagcc atcctgaata tatcccgctg ccccgggtgc
     3721 aggccgccct ttacaagccc gacagcggcc ctgcgccgca tgtggccttc ctggtggcgc
     3781 accgcaccgc caacaatctc tcgaccaatg cctgcgccga actcgcccag cgcggcttca
     3841 tggtgctctg cttcaacacc cgcttcgtca acaatgaggc acaggttcgc tgggaagaaa
     3901 ccccgctcga tgtgaaggcg gcggtggaat tcgtgcgccg gcagcccggc atcacgaagg
     3961 tggtgctgct cggccattcg ggtggctccc cgctgatgag cctctatgag gccattgccg
     4021 agaacggggc cgcttactgc cagcgcccgg aacgcatcgt gaaatgcggc aacgatgtcg
     4081 atggcctcaa acccgtggac gccatcgtct ttcccgatgc gcatcccgga aatccggtgc
     4141 aggcgctgcg cgatctgaac ccctcggtgg tgatgggcga ggacggcaag cttaaggtga
     4201 tccccgagct ggacccgttc agcccggcca acggcttcaa cccgaacggc gtctcgcatt
     4261 attccaagga attccagacc cgctattacg cggcgcaatc caaggccatg accgaccggc
     4321 tggcgcgggt tcaggcggac gaggcgcgca tcagacgcgg cgattatccg tatccggacg
     4381 atggcctcgt aatgattccg ggcggcggca atcccggcgc gggtgcgggc ggcgacggcg
     4441 tgctgatcgc catggacccg gatatcccgg aactgatgtc caccgcgcgg cccgagaagc
     4501 tgctcaagaa cgacggcacc atcgtgacgc agatcgtgca cagcgtcgcg gtgcccgaac
     4561 cccgcaacgc caagaccaat cgcgccttcc acaccggcac gaagatcttc accttcaaat
     4621 ccttccttag cgccaatgcg acgcgctcca ccaattcgct ggacgggatc gaggattgct
     4681 cgtccaacaa ttcgaccgtc tgcgcggtac aatcgatcca cgtgccgatc ctggtcgccg
     4741 ccatgggcgg ttatcatttt gtgcgggatc aggaggtcat ctatgacaaa tccgccagca
     4801 ccgataagga ttacgtcgtg atcgaaggcg cgctgcacgg ctacacgccc tgcacggctt
     4861 gcgaaaagac gccggggcaa tattccaaca cgatgaagaa cctgttcgac tacatcgccg
     4921 cctggaccaa taagcgattc tgaaaaggtt tcgattatga tgaaaaccct tggcctcatc
     4981 gccaccgcac tctcgttcac cgcgtccgcc gcttttgcgc agacgacctg gccgaaccag
     5041 aaggacgcat ttttcaccat caaggatttt catttcgcca atggcgagac gctgcccgag
     5101 ctgcggctgc attatgtgac cttcggcacg ccgaagaaga acgccgccgg cgagatcatc
     5161 aatggcgtga tcctgctgca cggcaccagc ggggcgggca aaagctggtt cctgcccagc
     5221 cttgccgacg agctgtacaa acccggccag ccgctgagtg cggaggatta tttcatcatc
     5281 gttcccgatg cgatcggcgt cggcggttcg tccaaaccga gcgacgggct gaaaggaaaa
     5341 ttcccccatt accgctatca cgacacggtg gaggccgaac atcgcctgat caccgaagcg
     5401 ctcggcatca agcatctgcg cctcgttctg ggcagttcca tgggcgcgat gcagacctgg
     5461 atgtgggggg agatgtatcc cgatttgatg gacggactgg tcccgctcgc gagccagccc
     5521 atcggcatat cggggcgcaa ctggatcaac cgccgcatcc gcatcgaggc gattaagcac
     5581 gatcccgatt acaacaacgg catgtatgac aagccgccca cgcattggat ctggacggcg
     5641 gcgtccggcc cgatgggcac cgaaagcgat taccagatcc agcagatcgc gccgacgcgt
     5701 gaggccggcg atgcctttta caagaagctg gtggaggacg cgtccaaggc cgatgccaac
     5761 gacacgctct acggcatcga agcggtggag gattacaacc ccacgcccga tctgcacaaa
     5821 atcaaggcga agctgatggc gatcaattcg gccgatgacg aggccaatcc ggcggcgctg
     5881 catgtgatgg agccggcgat cgcgaaaatc cccggcgcgc gctatgtgct gattccacaa
     5941 agcccggaga cgcacggcca ttacacgcac ttacgtgcgg cgatctggaa accctatctg
     6001 gtggaattcc tgaagacgct cccgccgatg tagtgacgcg gggcgcgcgg gacgtagcag
     6061 tctctaaaca cggtcacgaa ggggaaatcg tcatgaaggc ttggtcgaaa ttcatcgccg
     6121 tcacaatggc aggctgtttg agcgtactga ttgccggtgt cccggcgcgc gcaggcgaaa
     6181 tcagtgttct cgccggcggc ggcccgatgc ccgatgtcat gggcacgctc attccgatgt
     6241 tcgaaaaggc gacgggcaac aaggtgaaga tcgccttcaa gggcaatccc gcgattggca
     6301 tggacctgaa gcaaggtgcg gccgatctcg tcatcaccaa tagcgagttc gtggacgagc
     6361 ttgccacggg cggcgatctg gcggccaacg gcaagacgct gctgatgatt tcaaaagtgg
     6421 ccgtcgcggt gaagaaaggc gcgccgaggc ccgatgtcag cacacccgac aaactaaagg
     6481 ccgcactcct ggcggcgaag accgtcggct acagccaggg ttcaagcggc gtgcattttc
     6541 tgaccgtgat ccagaagctc ggcattgccg atgcggtcaa ggccaaagcc gtcatcgtgc
     6601 aaggccgtcc cgtgggcgcg gcgatcgcgg cgggcgaagc cgagatcggc gtgcagcaag
     6661 tcgccgaatt gcgtcccgtc gccggcgtcg atgtgattgg cgagctgccc cctgcgttgc
     6721 agaagcagat tccctattcc gccggcgtgg cggccaaggc gaaggacgcg gcaaccgccc
     6781 gcgcgctcgt ggccttcctg cgcacgcaac cggcgatgga cgtgctcaag cgcaaaggca
     6841 tggacctgcc gtaagaagcg cggcgtgaga ttattggaaa aaggggtggg cctcagcaaa
     6901 gcgaaattcg gggggaatct ttcgctgcta ggggcaaacc gctgaggccc aaggacaccc
     6961 taaacagccg aggtgagtgg cgtcaccaca cttcgccgta agcatcaggt cataaatatt
     7021 gcgtgtcacc ctcactttta agggagggtt agatgcggct ctgtaatgtg ggacgcaacg
     7081 atgccggaca acatactcgt cttctacggc tcttaccgcg ccacgcgcat tggcatccgc
     7141 atggcgagct tcgtcatcaa cgaacttcgc gcccgcggcg ccgtcccgga gctgattgac
     7201 gcccgtgaga taggacttcc gatcatcgac cggatgtata aggaatatcc cccgggttcc
     7261 gctccgaaag cgatggaaga cctcgctcga aaaatccgcg cggcggacgc ttttgtgttt
     7321 gtcgtgggtg aatacaattg gggaccgcag ccgggcctga aaaacctgac cgaccatttt
     7381 ttagaggaat ggttctggcg gccggccgcc atcgtcagct attcgccgag tcgactgtca
     7441 ggcgttcgtg ccagttcggc ctggcatacc atcctgtcag agatgggcat ggtcgtcgtc
     7501 tcgagcacag tggccgtggg ccccatagcc gagaccctcg acgcgcaagg cgagccgatt
     7561 ggcgaggtgg gccgcttggc acaaaaggca ctgcaccgcc ttgccgatga cctcgcgtgg
     7621 tggacctcgg ccgcccggaa cgaacgcctc caacgagcac cgccctatta gcgctttcgg
     7681 gccaaaggtt cgccgctatg gacggccgac gcggccgccc cttgtcaacc atatgtgaag
     7741 tccggcttgc tctgcccaat ttcgataatg taaccgtcag gatcgcggat gtagcagcgc
     7801 gtctcgccgt acttgtcctt cggctctgtg atgaactggg cgcctcgact tttccacaat
     7861 tcatagcagg cgcgaatatc cgcaacgcgg atattcatga agctgctgat cttatcaggg
     7921 tcggcgagga cgctgagcgt taccgacggc ttgtcgggcg tcggcccgcc cccgacattg
     7981 acgatgagcc acgtattcgc gatctgaatg taaccgggcg cgccgctgct atcgcctctg
     8041 ctgagaatgc gcccgccgaa gaccgtttcg tagaaatggg ccgatcggtc gatgtcggcg
     8101 acagtgagaa aatgtgcgac ggtgaacccg tcccgcggag gcatctcata ggttttctgt
     8161 tcgatttgta cattgctcat ggacagctcc cttttcgatc aagcgcccgt tcgggattaa
     8221 acttcccaaa tgcgtcacaa acatcccatc tctgggctgt tgccggttcg gttgacaccc
     8281 acttaagcta atccggcttt catctcgaac tccatagggc tgatatatcc gagcgtcgag
     8341 tg
//
LOCUS       DASYEG010000296         5134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_5039447, whole genome shotgun sequence.
ACCESSION   DASYEG010000296 DASYEG010000000
VERSION     DASYEG010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5039447"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2219)
                     /locus_tag="VGM72_12575"
     CDS             complement(<1..2219)
                     /locus_tag="VGM72_12575"
                     /inference="COORDINATES: protein motif:HMM:NF039196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEY4266147.1"
                     /translation="MAKAASATAATAPAALDRHQLLAVLRAFRKGDFSVRLPIGLTGI
                     DGEIGEAFNDVVELNERLTKEFERLGDVVGKDGKIGHRAKLPNATGSWASNVETVNNL
                     IADLVQPTSEMARVIGAVARGDLSQTVNLDIEGRPLRGEFLRIGKVVNTMVDQLGSFV
                     SEVTRVAREVGTEGKLGGQAKVKGVAGTWKDLTDNVNAMAANLTGQVRNIAEVTTAVA
                     NGDLSKKITVDVKGEVLELKSTINTMVDQLNSFASEVTRVAREVGTEGKLGGQANVRG
                     VAGTWKDLTDNVNLMAANLTGQVRNIAEVTTAVAKGDLSKKITVDVKGEILELKSTIN
                     IMVDQLNSFASEVTRVAREVGTEGKLGGQAKVEGVAGTWKDLTDNVNLMAANLTGQVR
                     NIAEVTTAVATGDLSKKITVDVRGEILDLKNTINTMVDQLNSFASEVTRVAREVGTEG
                     KLGGQANVRGVAGTWKDLTDNVNLMAANLTGQVRNIADVTTAVANGNLSKKITVDVKG
                     EILELKNTINTMVDQLNSFASEVTRVAREVGTEGKLGGQAKVEGVAGTWKDLTDNVNL
                     MAANLTGQVRNIAAVTTAVANGDLSKKITVDVKGEILELKNTINTMVDQLNSFASEVT
                     RVAREVGTEGRLGGQANVRGVAGTWKDLTDNVNAMATNLTGQVRNIAEVTTAVANGDL
                     SKKITVDVRGEILELKNTINTMVDQLNSFASEVTRVAREVGTEGKLGGQANVRGVAGT
                     "
     gene            2646..2948
                     /locus_tag="VGM72_12580"
     CDS             2646..2948
                     /locus_tag="VGM72_12580"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEY4266148.1"
                     /translation="MPTSSGHTAAILASKVKGTPVYNNNGDKVGTLEDIVLDKTSNNI
                     MFAVLGSGGMFGMGEKYRPIPWSVLDYDSEKGGYIVPLDKTMLEDAPARPDAGRWP"
     gene            3126..3308
                     /locus_tag="VGM72_12585"
     CDS             3126..3308
                     /locus_tag="VGM72_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004153593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEY4266149.1"
                     /translation="MLRWAVIFLIVGLIAGMLGFTGIAGASVGIAKFLFFLFIAMFLV
                     FLLLGLTVFRAITGPR"
     gene            complement(3332..4339)
                     /gene="cydB"
                     /locus_tag="VGM72_12590"
     CDS             complement(3332..4339)
                     /gene="cydB"
                     /locus_tag="VGM72_12590"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010423624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEY4266150.1"
                     /translation="MMAMLDLPLLFALTVAFSVGLYVLLDGLDLGIGILFLLAPDDAA
                     RDTMMNSVGPVWDGNETWLVMGGVLLFAAFPLVYAVVLPAFYVPLMLMLFALVFRGVA
                     FEFRFRAHRSRFLWDWAFSLGSGLAALMQGMMLGAFIDGISVRDGHFAGSTFSFLTPF
                     ALAAAFGVAAGYALLGAAWLIYKTDGPAQVFARGIAPWTLAATVVFIGIVSIWTPLAH
                     ANIAQRWFSLPNIFFLWPVPLVTGALALGIWRAIPQRRDGRPLLLSVLLVLMGYAGLG
                     ISLWPYAIPQSVTIWQAAGAPDTLMFLGIGTAIILPVTLAYLGYAYWTFRGKVGAGYE
                     R"
     gene            complement(4336..>5134)
                     /locus_tag="VGM72_12595"
     CDS             complement(4336..>5134)
                     /locus_tag="VGM72_12595"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY4266151.1"
                     /translation="FVVIGVMGWYLWRRQHLAFARAGFSVAMGIAAVLAPLQLAIGDA
                     HGLNSLAYQPVKVAAMEGDWETGPHQTLTLFAWPDMARERNAFALAIPELGSLILTHR
                     SDGVVQGLKSVAPGDRPYVPLVFFAFRIMVGIGFLLIAIAGTGLWLRWRGRLYDSAWF
                     SFLCAFSSPLGFIAILSGWTVTETGRQPWTVYGQLRTTDAASPVAAGAVATSLVLFII
                     VYCVLLVAFFYYAGRIVLAGPHPPKPAEEALSVRPGRDSAPVRGPAE"
BASE COUNT         1027 a         1657 c         1480 g          970 t
ORIGIN      
        1 gtgccggcga cgccgcgcac attggcctga ccgccgagct tgccttcggt gcccacttcg
       61 cgggccacgc gcgtcacttc ggaggcaaag gagttgagct ggtccaccat ggtgttgatg
      121 gtgttcttga gttcgagaat ctcgccgcgc acgtccacgg tgatcttttt cgacagatcg
      181 ccattggcca cggccgtcgt cacttcggcg atgttgcgca cctgtccggt gaggttggtg
      241 gccatcgcgt tcacattgtc ggtcaaatcc ttccaggtac cggccacgcc ccgcacattc
      301 gcctggccgc caaggcgccc ctcggttccc acttcgcgcg ccacgcgggt cacttcggag
      361 gcaaatgagt tgagctgatc caccatggtg ttgatggtgt tcttcaactc caggatttca
      421 cctttcacgt ccacggtgat ctttttcgac aggtcgccat tcgccacggc ggtcgtcacc
      481 gcggcgatgt tgcgcacctg ccccgtcaag ttcgcggcca tcaggttcac gttgtcggtc
      541 agatccttcc aggtaccggc gaccccttcc accttggcct gaccgccgag cttgccttcg
      601 gttcccactt cgcgggccac gcgagtcact tcggaggcga acgagttcaa ctgatccacc
      661 atggtgttga tggtgttctt cagctccagg atttcgccct tcacgtccac ggtgattttc
      721 ttcgacaaat tgccgttggc gacggcggtc gtcacatcgg cgatgttgcg cacctgtccc
      781 gtgaggtttg cggccatcag gttcacattg tcggtcaaat ccttccaggt accggcgacg
      841 ccgcgcacat tcgcctgacc gccgagcttg ccttcggtgc ccacttcgcg cgccacgcgg
      901 gtcacttcgg aggcaaacga gttcaactga tccaccatgg tgttgatggt gttcttgagg
      961 tcgagaatct cgccgcgcac gtccacggtg atctttttcg acagatcgcc ggttgccacc
     1021 gccgtcgtca cttcggcgat gttgcgcacc tgccccgtga gattcgcggc catcaggttc
     1081 acgttgtcgg tcaggtcctt ccaggtgccc gcgacgcctt ccaccttggc ctggccgccg
     1141 agcttgcctt cggttcccac ttcgcgggcc acgcgcgtca cttcggaagc gaacgagttg
     1201 agctggtcca ccatgatgtt gatggtcgat ttcagctcca ggatttcgcc cttcacgtcc
     1261 acggtgatct ttttcgacag atcgcccttt gccacggcgg tcgtcacttc cgcgatgttg
     1321 cggacctgcc ccgtgagatt cgcggccatc aggttcacgt tgtcggtcag gtctttccag
     1381 gtgccggcga cgccgcgcac attcgcctgc ccgccgagct tgccttcggt gcccacttcg
     1441 cgcgccacgc gcgtcacttc ggaggcaaac gaattcaact gatccaccat ggtgttgatg
     1501 gtcgacttca gttcgagcac ctcgcccttc acgtccacgg tgatcttctt ggacaaatcc
     1561 ccgttggcga ccgcggtcgt cacttcagcg atgttgcgca cctgaccggt gaggttcgcg
     1621 gccatggcgt tcacattgtc ggtcaaatcc ttccaggtgc cggcaacccc cttcaccttg
     1681 gcttggccgc cgagcttgcc ttcagtgccc acttcgcgcg ccacgcgcgt cacttccgag
     1741 acgaacgacc cgagctgatc caccatggtg ttcaccacct tgccgatgcg caggaattcg
     1801 ccgcgcagcg gccggccttc gatgtcgaga ttcacggtct gggaaagatc gccacgagcc
     1861 accgcgccga tcacacgcgc catttccgag gtgggctgca ccaggtcggc gataagattg
     1921 ttcaccgtct cgacgttgga cgcccacgag cccgttgcat tcggcagttt ggcccggtgg
     1981 ccgatcttgc cgtccttgcc tacgacatcg ccgagccgtt cgaactcctt ggtcagcctt
     2041 tcattgagct cgaccacatc gttaaacgcc tcgccgattt ccccgtcgat ccccgtcaag
     2101 ccgatcggca gacgcacaga gaaatcgcct ttgcggaatg cccgcaagac cgccagcaat
     2161 tgatgcctgt cgagcgcggc cggtgccgtc gcggccgtgg ccgatgctgc tttagccatt
     2221 cgaaactcct tggacaggtc tgcaaggaca cgaagaaacc gccgcggaac ggccgtgctg
     2281 acgccagcga aaacaagttc ttggaatcac ggcgcactca gccgtttgac cgctgcttag
     2341 gccgataatc ctgagcagag atgccctccc ggtaacctga aattcccccg catgcgacag
     2401 gcagccaacc ctcaaaccat caaacgcaag tccgcggcga aagttccatg aggttctacc
     2461 cagtacggaa aatattttct gcacggtatc gtacagccgg gcctaagcgt gatttccaaa
     2521 ccatcataac gcctggccct catgcaggac cggacgatcg gggtcggaac agcggtctgc
     2581 gcgcaaccgg aacatttcag gccatgaaaa gttaccgccc tgacgctcac tcaggagtaa
     2641 aaaccatgcc cacatccagc ggacacaccg cggcgatcct ggcttcgaag gtcaagggaa
     2701 cgccggttta taacaataac ggcgacaagg tcggaaccct cgaagatatc gttctcgaca
     2761 agacgtcgaa caacatcatg ttcgcggtcc tcggttcggg cggcatgttc ggaatgggcg
     2821 agaaatatcg cccgattccg tggtcggtcc tggactacga ttccgagaag ggcggctata
     2881 tcgtgcctct cgacaagaca atgcttgagg atgcacccgc cagacctgac gcggggcgat
     2941 ggccatgatg cgatccgcgc gaagaccttc aactattata acgcgccgca atattggtga
     3001 cgcgacacgt acatgcgttg cgtgcaattg aacgcctctc gagcccgctc gggaggcgtt
     3061 ttgcttctac ggaactcggg ctttgacgtt gcgttatctg agctgacaaa tccgaggaga
     3121 ctcaaatgtt gcgctgggca gtcatctttt tgatcgtcgg gctgatcgcc ggcatgttgg
     3181 ggttcaccgg catcgcgggc gcctcggtcg gaatcgcgaa attcctgttc ttcctcttca
     3241 tcgcgatgtt cttggttttc ctgctgctcg gcctgaccgt gttccgcgct atcaccggtc
     3301 cacgctgaac atccttagcg cgccttagaa ttcagcgctc ataaccggcg ccgaccttgc
     3361 cgcggaacgt ccaataggca tagccgagat aggcgagcgt caccggcagg atgatcgctg
     3421 tgccgatgcc taagaacatg agcgtgtcgg gcgcgcccgc cgcctgccaa atcgtgacgc
     3481 tttgtggaat cgcatagggc cacaggctga tcccgagtcc cgcatagccc atcagcacaa
     3541 gcagaaccga gagcagaagc ggccgcccat cgcgcctctg cggaattgcg cgccagatgc
     3601 ccaacgcgag cgcgcctgtc accaagggca ccggccacag aaagaaaata ttgggcagcg
     3661 aaaaccagcg ctgggcgata ttcgcgtgcg ccagcggcgt ccagatgctg acgatgccga
     3721 tgaaaacaac cgtcgcggcc aaagtccaag gcgcgattcc gcgcgcgaag acctgggccg
     3781 gtccatcggt cttgtagatc agccaggcgg cacccaggag cgcatagccg gcggcgacgc
     3841 caaacgcggc ggccagcgcg aacggagtga gaaaggaaaa tgtgctcccc gcgaaatggc
     3901 catcgcggac ggaaatgccg tcgatgaagg cgcccagcat catcccctgc atgagtgcgg
     3961 cgagccccga gcccaaagag aatgcccaat cccagagaaa ccgcgagcga tgcgcccgaa
     4021 accggaattc gaaggcgacg ccccggaaca ccagcgcgaa cagcatcagc atcagcggta
     4081 cataaaaggc cggcagaacc accgcataga ccagcggaaa cgccgcgaac aaaagcactc
     4141 cgcccatcac cagccaggtt tcgttgccgt cccagacggg accgacggaa ttcatcatgg
     4201 tatcgcgcgc ggcatcgtca ggcgccagca ggaacagaat tccgatgccg agatcgagcc
     4261 cgtcaagcag cacataaagc ccgaccgaaa acgcgacggt cagtgcgaag aggagcggca
     4321 gatcgagcat ggccatcatt ccgcaggtcc gcggacgggt gcggaatcgc gtcccggacg
     4381 tacgctcaac gcttcctcgg caggctttgg cggatgcggt ccggcgagca cgatccggcc
     4441 ggcataatag aaaaaggcca ccaacagcac gcaatagacg atgatgaaaa gcaccagaga
     4501 tgtcgcgacg gctccggccg cgaccggcga tgcggcgtcg gtggtgcgca attgcccata
     4561 gaccgtccag ggctgacggc cggtttcggt caccgtccag ccgctcagaa tcgcgatgaa
     4621 gcctagcggg ctggagaagg cgcagaggaa gctgaaccat gcgctgtcgt agagacggcc
     4681 gcgccagcgc agccatagcc ccgtccccgc aatggcgatc agaagaaacc ctattcccac
     4741 catgatgcgg aatgcgaaaa acacgagcgg cacatagggg cgatccccag gcgccaccga
     4801 tttcaaaccc tgaaccacgc catcgctgcg atgggtgaga atgaggctcc cgagttcggg
     4861 aatcgcaagc gcgaaagcgt tgcgctcgcg cgccatgtca ggccaggcga acagcgtgag
     4921 ggtctggtgc ggtccggttt cccaatcgcc ttccatcgcc gcgaccttga cgggctgata
     4981 ggcgagactg ttgagcccat gcgcgtcgcc gattgccaac tgcagcggcg ccagcacggc
     5041 ggcgattccc attgccaccg aaaagcccgc acgggcaaac gccagatgtt gccgccgcca
     5101 gagataccaa cccatcacgc cgatcacgac gaag
//
LOCUS       DASYEG010000297         2807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3738834, whole genome shotgun sequence.
ACCESSION   DASYEG010000297 DASYEG010000000
VERSION     DASYEG010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3738834"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2807
                     /locus_tag="VGM72_12600"
     CDS             <1..>2807
                     /locus_tag="VGM72_12600"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY4266152.1"
                     /translation="GAIAKALNYVYALCDNEQTRIHVVTMSMGGVASQAWADAINALY
                     DRGVFVVTAGGNNYANLPTHNIVYPARFNRVVAACGVMANGKPYADLDPRLMAGNYGP
                     SSKMKTAISAYTPNTPWARLGCAAIVDHDGAGTSAATPQVAAAAAIWIAKNQTAWKAY
                     PENWMRAEAVRTALFKTAKTDEEKHFGKGLLQAKEALSVVPAAGALTAQPKDSADFAV
                     LRILTGLGAAPPSSAQQRMLELEALQLAQTGEIEKILPDPQGPISVETARAVARALIT
                     DPRASEALRTTLAGALAQQPAPPSPGRRKKKTGDGTVTPPEPAHTPAPPGSSPLPGEF
                     SAIQQQQLRTALNPPIEKPKRRKLQVYAYDPSLGTSLDTLGINLALLDVRWETLEPGP
                     VGEYLEIVDADPASGACYAPVDLDDPHLLPQQGLAPTETDPRFHQQMAYAVASRTIEF
                     FERALGRVALWAPRTPRLPGESERYVGRLRLYPHALRGANAYYSPDKKALLLGYFNAS
                     DSDPSENAPGSLVFTAISHDIVAHETTHALLDGLHRRYIEATNPDVLAFHEGFADIVA
                     LFQHFTMPEALKTQIARTRGDLGRQSLLGALAVQFGHATGNYGALRDAIGTCDENGVW
                     HPHQPKTTDYTNATEPHDRGAVLVAAVFDAFLQLYRARTADLIRLATGGTGILGEGAL
                     PDDLTDRLAEEAATVARHCLEICIRGLDYCPPVDITFHDYLRAVITADRDIAQDDPLG
                     YRVAFVSAFRARGISSLDVRSISSDSLAWEPPPLQIGRLADVLKKLRLDWDLKSNREH
                     AYRASRHNAAIFHRWLVSDAVSDEELAMLGLVRHERPLTLNGVEGYLGPIEVHSVRPA
                     HRIGPDRESRAELILEITQTWKDKLSAPDTIFRGGCTMIVDLEKSAALYLVRKRVDHA
                     GRFAAQTSFQALTSLNPLRAN"
BASE COUNT          514 a          923 c          857 g          513 t
ORIGIN      
        1 ggggccatcg ccaaggctct caattatgtc tacgcgctct gcgacaacga acagacgcgc
       61 attcacgtgg tcaccatgag catgggtggc gtggcatcgc aggcatgggc ggatgcgatc
      121 aacgccctct acgatcgggg cgttttcgtg gtgaccgcgg gcggaaacaa ctacgccaat
      181 ttgccgacgc acaacatcgt ctacccggcg cggttcaacc gcgtcgtcgc ggcctgtggg
      241 gtcatggcaa acggcaaacc ctatgccgat ctcgatccgc ggctgatggc gggcaattac
      301 gggccatcat cgaagatgaa gacggcgatc tcggcctaca cgcccaacac gccctgggcg
      361 cgattggggt gtgctgcgat tgtcgatcat gacggggcgg ggacctccgc cgcgacgccg
      421 caggttgccg ccgccgcggc gatctggatc gcgaaaaacc aaaccgcgtg gaaagcctat
      481 cctgagaact ggatgcgcgc ggaggcggtt cgcaccgcgt tgttcaaaac cgcgaagacg
      541 gatgaagaga agcatttcgg caaagggctg ctgcaagcca aagaggcgct gagcgtcgtg
      601 cccgcggccg gcgcgttgac ggcgcagccc aaggacagcg ctgatttcgc ggtgcttcgg
      661 attctcaccg gcctcggggc cgccccccca tccagtgcgc agcagcgcat gctggaattg
      721 gaagcgctgc aacttgccca gaccggcgaa atcgagaaaa tccttcccga tccccaaggg
      781 ccgatttccg tcgagacggc gcgcgcagtg gcacgcgcct tgatcaccga tccgcgtgcg
      841 tcggaggcgt tgcgtacaac cctcgccggc gctctcgcgc aacagcccgc gcctccttcc
      901 ccgggccgcc gaaagaaaaa aaccggggac ggcacggtca ccccgcctga gcccgcccac
      961 actcctgcac cgccgggaag ttcgcctttg ccgggtgaat tttccgccat ccagcagcaa
     1021 cagcttcgaa cggcgctcaa tccacccatc gagaaaccga agcgccgcaa gctgcaagtc
     1081 tatgcctatg acccttcgct cggcacctcg ctcgacacgc tgggcatcaa tctggcgctg
     1141 ctcgatgtcc gatgggagac gctggagccc gggccggtgg gagaatatct cgaaattgtc
     1201 gacgccgatc cggcaagcgg cgcctgctat gcccccgtcg atctcgacga tccccacctg
     1261 cttccgcaac agggcttggc gccgacggaa accgatcccc gattccatca gcagatggcc
     1321 tatgcggtgg cgtcgcggac catcgaattc ttcgagcgcg cgttgggccg cgttgcgctg
     1381 tgggcgccgc gaacgccacg cttgcccggc gagtcggagc gttacgtggg acgcctgcgc
     1441 ctctatccgc atgcgctgcg cggtgccaat gcctattaca gccccgacaa gaaagcgctg
     1501 ctgctcggct atttcaatgc gagcgacagc gatccttccg aaaacgcgcc aggttcgctc
     1561 gtcttcaccg cgatttccca cgatatcgtc gcgcacgaga ccacgcatgc gctgctcgac
     1621 ggtctgcatc gccgatatat cgaggcgaca aatcccgacg tgctcgcctt ccatgaaggc
     1681 ttcgcggaca tcgtcgcgct gtttcagcat ttcaccatgc ccgaggcgct caagactcaa
     1741 atcgcgcgca cgcgcggcga tctcgggcgg cagagtctgc tgggggcgct tgccgttcag
     1801 ttcggccatg ccaccggcaa ttatggcgcc ctgcgcgatg cgatcggcac ttgcgacgag
     1861 aatggcgtgt ggcatcctca tcagcccaag acgacggatt acaccaatgc caccgagccc
     1921 cacgatcggg gtgcggttct ggtggcggcg gtgttcgacg cattcctcca gctttatcgc
     1981 gcacgcaccg ccgatcttat ccgcctggcg acgggtggca ccggcattct gggggaaggc
     2041 gcgctcccgg acgaccttac ggatcgtctg gccgaggaag ccgcgacggt ggcacgtcat
     2101 tgcctcgaaa tctgcattcg cgggctcgat tattgccccc ccgtagacat cacgtttcac
     2161 gactatctgc gcgcggtcat caccgcggat cgcgacatcg cgcaggacga tcccttgggc
     2221 tatcgcgtcg ccttcgtttc ggcgtttcgc gcgcgcggca tctcgtcctt ggatgtgcgg
     2281 tcgatttcgt cggacagttt ggcctgggag cctcctccgc tgcagatcgg caggcttgcg
     2341 gacgttctaa agaaattgcg gctcgattgg gatctgaaat ccaatcgcga acacgcgtat
     2401 cgcgcctcgc gccacaatgc ggcgatcttc caccgctggc ttgtgtccga tgccgtgagc
     2461 gacgaagagc ttgcgatgct gggcctcgtc cgtcacgagc ggcctctgac gctgaacggc
     2521 gtggagggat atcttggccc catcgaagtc cattcggtgc ggccggcgca tcgcatcggt
     2581 ccggaccggg aaagccgcgc cgaactcatt ctcgagatca cgcagacatg gaaagacaag
     2641 ctttccgcgc ccgacacgat tttccgcggc ggctgcacga tgatcgtcga tcttgaaaag
     2701 agcgcggcgc tttatctggt ccgcaagcgt gtcgaccatg cggggcgttt cgccgcgcag
     2761 acgtctttcc aggctctgac atccctgaac cctttgagag cgaatta
//
LOCUS       DASYEG010000298        13217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4027362, whole genome shotgun sequence.
ACCESSION   DASYEG010000298 DASYEG010000000
VERSION     DASYEG010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 13217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13217
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4027362"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VGM72_12605"
     CDS             <1..219
                     /locus_tag="VGM72_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266153.1"
                     /translation="RIAQVGARLIDATAKSLAEQFFKKFAAVVEKPQAAAEPMRETTR
                     EPSRNLGLFARIWAAIVRFLSGPAKTKD"
     gene            379..963
                     /locus_tag="VGM72_12610"
     CDS             379..963
                     /locus_tag="VGM72_12610"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEY4266154.1"
                     /translation="MPEGLDLGPQIAVDTETLGLNPVRDRLCLVQLSSGDGSAHLVQF
                     SRDRYQAPNLKRLLNDRNVLKLYHFARFDLATMRRYLGVTAGPVYCTRTASKLARTYT
                     DKHGLKDLVKELLDVDLSKQQQSSDWGAETLTEQQLAYAANDVAFLHRLMEALDRMLI
                     REGRMTLAQACFDFLPARAELDLAGWAEGDIFAH"
     gene            1165..2187
                     /locus_tag="VGM72_12615"
     CDS             1165..2187
                     /locus_tag="VGM72_12615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEY4266155.1"
                     /translation="MSMPKAKDLSAENREESDLKAARRVLRFAGEAFSMLSANLDGAF
                     TRALDLLAAVRGRVVVSGMGKSGHIGRKLAATLSSTGTPAQFVHPAEASHGDLGVLTR
                     SDALIMLSNSGETAELSDLITYAKRHAIPLIGIASNPDSALIAASDAALVLPRVQEAC
                     PMGLAPTTSTTLMMALGDALAVALMERKGFSADQYRDLHPAGSLGRALIRVSDLMHKE
                     GEFPLVTADTPMKQILILMTSFGRFGFAGVVGIQDDSGKLIGIFTDGDLRRHIHANPL
                     DHVAADLMTKNPKTIAAGKLAAEALAEMNAQRITSLFVVADDAAAANIPIGLIHVHDC
                     LRAGLR"
     gene            2187..2906
                     /gene="lptC"
                     /locus_tag="VGM72_12620"
     CDS             2187..2906
                     /gene="lptC"
                     /locus_tag="VGM72_12620"
                     /inference="COORDINATES: protein motif:HMM:NF018537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEY4266156.1"
                     /translation="MSPAQSDQPKTRKEPLLSYRAREWRISPRATLIAARRYSMFVKF
                     MKGALPLAALALGIAVLAYALQPRDARRMAMTFERLSRVEGDLAMLKPRLSGVDNDGL
                     PFVITAGRAIQEARGSDRVRLEDVAADMNLKDGTSLHVTAVKGVVDTKTHVLQVSGGM
                     HLTSKDGYDARAQSAVADLKAGTVHGERGIEAEGSFGHITAERFALNRDTRQLRFSGK
                     VHMVINADASRKTAPTGGILQ"
     gene            2903..3487
                     /locus_tag="VGM72_12625"
     CDS             2903..3487
                     /locus_tag="VGM72_12625"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEY4266157.1"
                     /translation="MRRFAALTLAFGLAVTMTDAASSAPAPARRGPNAGLDSNAPIAV
                     NADSFLADLNGETGTYSGNVIVTQGTVRLHADTVKVTAPSGHASKMEAQGHVVVDSPS
                     GQAVGDNGFYDVPSQVLRLNGNVVLTKEANVMRGSALEISMATGIARLTAGGQPALPG
                     QPATQPQGRVQGLFIPAQRSGAAPPPANANSQNP"
     gene            3530..4324
                     /gene="lptB"
                     /locus_tag="VGM72_12630"
     CDS             3530..4324
                     /gene="lptB"
                     /locus_tag="VGM72_12630"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEY4266158.1"
                     /translation="MNDNTNIYDLRDGAERPAPRLLEGFQGLAVARIGKSFKKRPVVR
                     GVSLTLQRGEVVGLLGPNGAGKTTIFYLITGLIRADYGSITLDGHDITRLPMYRRARL
                     GIGYLPQEASIFRGLTAEENIRAAAELVEPDSAHCATLVDSLLAEFSISHVAHTPAIS
                     LSGGERRRVEIARALATHPAYILLDEPFAGVDPIAINDIRELVRHLKDRGIGVLITDH
                     NVREALEIIDRAYIVHEGQVLMEGTPADIVGDKDVRRVYLGERFSL"
     gene            4346..5869
                     /gene="rpoN"
                     /locus_tag="VGM72_12635"
     CDS             4346..5869
                     /gene="rpoN"
                     /locus_tag="VGM72_12635"
                     /inference="COORDINATES: protein motif:HMM:NF004596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEY4266159.1"
                     /translation="MALTQRLELKQGQTLVMTPQLQQAIKLLQLSNLELADYVETELE
                     KNPLLEREDPHAGEGEPNPREIEGAAAIADNAPLDKTLATEDFGTASDLDAGHDDLYA
                     DESRAEKQPQGEINQPLTDWSNTGSGGLGDGEDFEGSLTAGTTLKGHLEAQLAIAGLA
                     PHQRFLAEVLIEAVDEAGYLRADLAEIAERLGATREDCDSTLAALQGFDPAGVFARDL
                     VECLALQLKERDRLDPAMQLLLTRLDLVAKRDIAGLSALCCLDASDISDMIAEIRALT
                     PKPGLAFGSEPVQPVVPDVYVREGADGSWHVELNSDTLPRVLVNARYYAQVSRSARNR
                     DDKSYLTDCMNSANWLVKSLDQRARTIVKVASEIVRQQDAFLTHGVRHLRPLNLKLIA
                     DAIGMHESTVSRVTSNKYMATPRGLFELKYFFTSAIQSANGGEAHSAEAVRDRIRELV
                     EKEEDDVLSDDRIVTILSGDGIDIARRTVAKYREALRIPSSVERRRQRQLEVSDAGA"
     gene            5969..6301
                     /gene="raiA"
                     /locus_tag="VGM72_12640"
     CDS             5969..6301
                     /gene="raiA"
                     /locus_tag="VGM72_12640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEY4266160.1"
                     /translation="MMHIRIAGKQIEIGEALPQQVRERLSETVEKHFDRDATANVTFT
                     KERMGFRADCTVHLSSGASMQAHGIAEHAHAAFGIAREHLEKRVRRYKRRLKNHHDRG
                     GLSPSASL"
     gene            6316..6780
                     /gene="ptsN"
                     /locus_tag="VGM72_12645"
     CDS             6316..6780
                     /gene="ptsN"
                     /locus_tag="VGM72_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008035594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEY4266161.1"
                     /translation="MEIADLLSPDAVLAHVKASTKKQVLQEMAHKAAMLTQLPERRIF
                     EILTEREKLGSTGMGQGIAIPHGRVAGVEKMTGLFAQLDHPVDFDSMDDQPVDLVFLL
                     LAPEGAGADHLKALARVSRLLRNQTICEKLRAAPQAATLYALLTEPSAAQAA"
     gene            6852..7979
                     /locus_tag="VGM72_12650"
     CDS             6852..7979
                     /locus_tag="VGM72_12650"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HEY4266162.1"
                     /translation="MAVAMGIGRFFYTPLLPIMQRQLRFGADIAGLIASVNFVGYLLG
                     TVMAAFVPKGRTRLLVFRFALIASVATTFATGLTESVSLWLVLRGAAGVASAFVFLFA
                     AAIVAETLTATGKPGHVGWLFGGVGIGIALSGLIVRIASGVLDWAGLWIAAGALSVLA
                     VPFIFSAVRDMTLAPDPSREKIAHRVPRPLPLWPLLVNYTCEGFGYSIMATFIVAIVK
                     ARPGMEAVGDWVWVVVGLAGLPSTMFWSWIAERIGYAVALTLAYVAQLIGILLPVVSD
                     LPSVALLAAILFGGTFMAITALVLALGRESANGQGFAVLTAGFGGGQILGPLAAGYLV
                     AGRTDFHQALLVSAGVILAGLFFLALAIMQARVRERMVNAR"
     gene            complement(7966..8247)
                     /locus_tag="VGM72_12655"
     CDS             complement(7966..8247)
                     /locus_tag="VGM72_12655"
                     /inference="COORDINATES: protein motif:HMM:NF018343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1150 family protein"
                     /protein_id="HEY4266163.1"
                     /translation="MRQEEEIFDDDSPETEELEGPAFEGARLAYIKPIALDEAKAMGL
                     VIPSEIKLPANVKLYALHAADGRTLGITDNWASAYSAAVQNNFVPVSVH"
     gene            complement(8261..8695)
                     /locus_tag="VGM72_12660"
     CDS             complement(8261..8695)
                     /locus_tag="VGM72_12660"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEY4266164.1"
                     /translation="MNRTFFNNPFLLGFEHIEKLLERTAKADSYPPYNIEHITENRLK
                     ITLAVAGFRAEDLSVTMEDNQLVIRGRMTDPEDKTFLHRGIASRQFQRNFVLAERIEV
                     LSAELRHGLLTIELERPHQESVVRTIPIHSADVHSSNGNNRT"
     gene            8866..10635
                     /gene="pabB"
                     /locus_tag="VGM72_12665"
     CDS             8866..10635
                     /gene="pabB"
                     /locus_tag="VGM72_12665"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEY4266165.1"
                     /translation="MRAPFVLLDDSRSEQQRSRLFTGLVRTVEARTASEVQPALDALA
                     AARKEGLFAAGYFAYELGYALESRLVPLLPQSSIPLLWFGLFHSQETLAGPETDRWLA
                     RQCRGRAYAGPLHPGDTEKEYAGRFARVRDDILAGDAYQVNLTFACRFAFAGDALALY
                     ARLRRQAKAGHGAYVDDGTRSILSLSPERFFAVADGVLTARPMKGTAPRGKDAASDAR
                     IAAGLQTSEKDRAENLMIVDLIRNDIGRVAKTGTVKVEDLFAVETYPTVHQMVSTVRG
                     ELRAGVTPADLVRALFPCGSVTGAPKLRAMEIIAELERTPRGLYCGAIGVFAPDGGSD
                     FNVAIRTLTIEGREGRLGIGGGIVADSKPDAEYEECLIKARFFGEGRPPLSLIETLRY
                     EPRQGFVREPLHLARLARSAATFGFSFDEKTARNVLRDCVTHTTASLRVRLQLDEDGS
                     FTTSIAAFVPAAAGTVWTYKISAHQVQSADVLSRHKTNWRTLYDEERAAANTSGCDEV
                     LFRNERDEIVEGSTTNLFVRIGDRLVTPVLSCGPLEGCLRRALLDAGECVEGIILASD
                     LEKNEVYLGNSLRGLIGARSFNG"
     gene            complement(10638..11420)
                     /locus_tag="VGM72_12670"
     CDS             complement(10638..11420)
                     /locus_tag="VGM72_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY4266166.1"
                     /translation="MISLQTLGALLSGGAVGFSLGIIGGGGSILAVPLLLYVVGVQDA
                     HIAIGTSALAVAVNAFINLLSHWRAGNVHWPCAILFGSAGMLGAVIGSTLGKLVSGEH
                     LLFLFAMVMIAAGIAILRPRNGGGEICRRMTSRIVFRLVIVGLAAGTISGFFGIGGGF
                     MIVPGIMYGGGLPLLNAIGSSLFAVGTFGLTTAVNYALSGFIDWGTATEFILGGAIGG
                     VFGMRAAIRLAAKKQALNYIFAGAVFAVAAYMLTRTGLDLFA"
     gene            complement(11417..12382)
                     /locus_tag="VGM72_12675"
     CDS             complement(11417..12382)
                     /locus_tag="VGM72_12675"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY4266167.1"
                     /translation="MGRRTAIIFSFALALFGMPEALGQTTEVWPQPNPDSLVPGAGKD
                     IVVYGQRVFNETYSVIGPEVPDTSMRYAGNNLSCQSCHLKGGTQKFAIPMIGIWGVFP
                     VYIARENEVRTLEERIEGCMERSMNGRALPLGGKEMKALLAYIQFLSTNVPVGKSLEG
                     RASPSLPLLSRAADPKHGSQIYEKECAFCHQADGQGQRKGPPGEAKGYLYPPLWGPDS
                     FNDGAGMHRLIGSASFIHANMPFGTHYDAPQLSVEDAWDVAAYINSQPRPKRANLDAD
                     YPDRARKPADAPFGPFTDKFPPEQHRLGPFQPIIDAQRAASKAQH"
     gene            12500..12964
                     /locus_tag="VGM72_12680"
     CDS             12500..12964
                     /locus_tag="VGM72_12680"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEY4266168.1"
                     /translation="MFKRLRAAVALMAVAIPVFLAGMHTASSQNTPKLHKMVIQVSRE
                     DTEGMMLALGNAINAKKLYDARGEQFQVEIVAYGPGITMFREDKSPVKERLAEAKKEI
                     PGIVYSMCGNAKAAAEKREGHEVVPIAGVGVVPAGIVRVAELQEQGYVYIRP"
     gene            12986..>13217
                     /locus_tag="VGM72_12685"
     CDS             12986..>13217
                     /locus_tag="VGM72_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266169.1"
                     /translation="MAFISRRRFALGAGAAATTALVGPRRGRAAAPIEMRQFHNQTEA
                     TPLDKRLKEMWAAVEMETGGKIRVKTYARNDNI"
BASE COUNT         2563 a         3969 c         4030 g         2655 t
ORIGIN      
        1 agaatcgctc aggtgggcgc acgcctcatc gatgccaccg cgaaatctct ggcggagcag
       61 ttcttcaaaa aattcgccgc cgtcgtggaa aaaccgcaag ccgctgcgga acccatgcgc
      121 gaaaccacgc gtgagccaag ccgcaatctt ggcctttttg cccgtatctg ggctgcgatt
      181 gtgcgtttct tgtccgggcc ggccaaaacc aaggattaga aggcgccaag acgtcgtctc
      241 gtcggtgcaa gaaattactg tctctccaac ctcttgatat gctttaacgc tccaggctcg
      301 catgtgctct gacgggtatt ctgcgcgcgc ccatcgcgaa ggtagcaccg tttgaaaatc
      361 cgtctccaca gggacgactt gcctgagggc ctcgatttag gcccccaaat cgccgtcgat
      421 accgaaaccc tggggctgaa cccggtgcgc gaccggctct gtcttgtgca gctttcttcc
      481 ggggatggca gcgcccatct cgtgcagttc agccgcgatc gctatcaggc acccaatctc
      541 aagcgcctct tgaacgatcg caacgtattg aagctctatc acttcgcccg cttcgatctc
      601 gccaccatgc gccgttattt gggcgtgaca gccgggccgg tctactgcac ccgcaccgcc
      661 tcgaagcttg cgcgcactta caccgataag catggtctca aggatctggt gaaggaactt
      721 ctggacgtcg acctttccaa gcagcagcaa agctcggact ggggcgcgga gaccctgacc
      781 gaacagcagc tcgcttacgc tgcgaacgac gtggcatttc tgcaccggct gatggaggca
      841 ctggatcgga tgctcatccg ggaaggacgc atgacgctgg cacaggcctg cttcgacttt
      901 cttcccgcgc gggcggaact cgatcttgcc ggttgggccg aaggcgacat cttcgcccat
      961 tagctgccgg cccggtgagc aaagactaag gccttttcag ggcgtgatta ttttcacttt
     1021 ggtcggtttt ctgaccgtcc ggccttgatc gttcccaccc ggctgctcat aatcaccgtg
     1081 agacttggca tgattttcgc ttgtggcgcg gctttcgcgc gggcgcagcc agtcggaaca
     1141 agcatttttc cggtgagccg ctctatgtcg atgcccaaag ccaaggatct ttccgcggag
     1201 aatcgcgaag agtccgacct gaaggccgcc cgcagggttc tgcgctttgc cggggaggca
     1261 ttctccatgc tttcggccaa tctggacggg gcgttcactc gtgcgctcga tctgctggcg
     1321 gcggtgcggg gacgcgtggt ggtcagcggc atgggcaaga gcggccatat cgggcgcaag
     1381 ctcgcggcca ccctgtcctc gaccggcacg cctgcgcaat tcgtacatcc cgccgaggcc
     1441 agccatggcg atctcggggt gctcacgcgc tccgatgctc tcatcatgct gtccaattcc
     1501 ggggaaacgg ccgagctttc cgacctcatc acctatgcca agcgccatgc catccctctt
     1561 atcgggatcg cgagcaatcc cgattccgcg ctgatcgcgg cctctgacgc cgctctggtg
     1621 ctgcccaggg tccaggaagc ctgccccatg ggccttgcgc cgaccacctc cacgacattg
     1681 atgatggcgc tcggcgacgc gcttgcggtc gcactgatgg aacgcaaggg cttttccgcc
     1741 gaccaatacc gcgatcttca tcccgccgga tccttagggc gcgccctgat ccgggtgtcc
     1801 gatctgatgc ataaggaggg ggagtttcct cttgtgaccg ccgacacccc gatgaagcaa
     1861 atccttatcc tcatgacgtc gttcgggcgc ttcggcttcg ccggcgtcgt gggcatccag
     1921 gacgacagcg ggaagctgat cggcatattc acggacggcg atctgcgccg ccatattcat
     1981 gccaacccgc tggatcatgt tgcagccgac ctgatgacca aaaatcccaa gacgatcgca
     2041 gccggaaagc tcgccgcaga agcgcttgcg gagatgaacg cgcaacgcat caccagtctt
     2101 tttgtggttg ccgacgacgc agcagccgca aacatcccca tcggcctcat tcacgttcat
     2161 gattgcctgc gggcaggact gcgctgatgt caccggctca gagcgatcag cccaagactc
     2221 gcaaggagcc gcttctttcc tatcgcgcgc gcgaatggcg catctcgccg cgtgcgacgc
     2281 tgattgccgc gcgccgttac agcatgttcg tgaaattcat gaagggcgcc cttcctttgg
     2341 cggctctcgc gctcggcatc gccgttctgg cctatgcgct gcagccccgc gatgccagac
     2401 gcatggccat gacgttcgag cgtttgtcgc gcgtggaagg cgatctggcc atgctgaagc
     2461 cgcgtctttc cggtgtcgac aatgacggac ttccttttgt catcactgct ggccgggcga
     2521 tccaggaagc gcggggctcc gatcgcgtgc ggctggagga tgtcgccgcc gatatgaacc
     2581 tgaaggatgg aacctcgctc catgtgaccg ccgtgaaagg tgtggtcgat accaagactc
     2641 atgtgctgca ggtctccggc ggaatgcatc tgacgtcgaa agacggctac gacgcgcggg
     2701 ctcagtcggc cgtggccgat ctcaaggccg gcactgtgca cggcgagcgt ggcatagaag
     2761 ccgaaggaag ttttggccac atcaccgctg agcgtttcgc gctcaatcgc gacacgcggc
     2821 agcttcgctt ctccggcaag gtccacatgg tgatcaacgc agacgcaagc agaaaaacgg
     2881 caccgacggg agggattctg caatgaggcg tttcgctgcg ttgaccttgg cttttggtct
     2941 tgccgtgaca atgacggacg ctgccagttc ggcgcccgcg ccggcacggc gagggcccaa
     3001 tgccggcctt gattcgaacg cccccattgc cgtcaacgcc gattcttttc tagccgatct
     3061 caatggagag accggcacct atagcggcaa tgtcatcgtt actcagggca cggtcaggct
     3121 gcacgccgac acggtgaagg tcaccgcacc cagcggtcat gcttcgaaga tggaagccca
     3181 gggccatgtg gtggtggact ctccatcggg tcaggcagtg ggcgacaatg gtttctacga
     3241 tgtgccgtcg caggtgctgc gcttgaacgg caatgtggtt ctgaccaagg aagcgaatgt
     3301 gatgcgcggc tcggcgctgg aaatttccat ggcgaccggc attgcgcgcc tgaccgcagg
     3361 cggacagccc gctttgccgg gtcagccagc cacgcagccc cagggccggg ttcagggcct
     3421 cttcatcccc gcccagcggt cgggtgccgc cccgccgccg gcgaatgcaa attcacaaaa
     3481 tccctaaagt tcacgcaatc ttaaacccac gaaagcaaaa tccgggccaa tgaacgacaa
     3541 cacaaacatc tacgatttgc gtgacggtgc cgaaaggccg gcgccgcgac tcttggaagg
     3601 tttccagggt ctggccgtcg cgcgaatcgg aaagagtttc aaaaaacgcc ccgtggttcg
     3661 tggcgtgagc ctgaccttgc agcgtggcga agtggtcgga ctgctcggtc ccaacggggc
     3721 gggcaagacc acgatcttct atctcatcac cggtctcatt cgcgccgatt atggctccat
     3781 aacgctcgat ggtcacgaca tcacgcggct tccgatgtac cgccgcgcgc ggcttggcat
     3841 cgggtatctt ccgcaggaag cttcgatctt ccgtggactg acagccgaag aaaacatccg
     3901 cgccgccgcc gagctggtcg agccggactc cgctcattgc gccactctgg tcgacagcct
     3961 gctggcggag ttttccatct cccatgtcgc gcacaccccg gcgatttccc tgtcgggcgg
     4021 tgaaaggcgg cgcgtggaga tcgcccgcgc gcttgcgacg catcctgcct atatcctttt
     4081 ggacgagcct ttcgcaggcg tcgatccgat cgcgatcaac gatatccgcg agcttgtgcg
     4141 gcatctgaag gaccgtggca tcggggtcct catcaccgac cacaatgtcc gcgaggcgct
     4201 tgaaatcatc gatcgcgctt acatcgtcca cgaaggtcag gtgctcatgg agggcacgcc
     4261 ggccgatatc gtgggcgaca aagacgtccg ccgggtctat ctcggcgagc gcttctcttt
     4321 gtagaggcgg cgccctgggg caattatggc gctgacgcaa cgcctcgaac taaaacaggg
     4381 ccagaccctg gtcatgacgc cgcaattgca gcaggcgatc aagctgctgc agctctccaa
     4441 tctggagctg gcggactatg tcgagaccga gcttgagaaa aacccgttgc tcgagcgcga
     4501 ggaccctcac gccggcgagg gtgaacccaa tccccgggaa atcgaaggcg cggccgcgat
     4561 tgcggacaat gcgccgctcg acaagacgct cgccacagaa gatttcggca ccgcctcgga
     4621 tctcgatgcc gggcacgatg atctttacgc ggatgagagc cgcgcggaga agcagccgca
     4681 gggcgagatc aaccagccat tgaccgattg gtcgaacaca ggcagcggcg ggttgggaga
     4741 cggcgaggat ttcgaaggca gcctcaccgc cggcacgacg ctcaagggac atttggaagc
     4801 acagcttgcc atcgccggat tggcgccgca tcagcgcttc ctcgccgagg tgctgatcga
     4861 agcggtggac gaagcaggtt atctgcgtgc cgatctcgcg gagatcgccg aacgcctggg
     4921 cgcgacgcgc gaagactgcg actctaccct cgcagcactg caaggcttcg atcccgccgg
     4981 ggtgttcgca cgcgatcttg tcgaatgcct ggcgctgcaa ctgaaggagc gcgaccggct
     5041 cgatccggcc atgcagcttt tgctgacgcg gctcgatctc gtcgccaagc gcgacatcgc
     5101 cggtctctcg gcgctttgct gtctggatgc atccgacatt tcagacatga tcgcggagat
     5161 tcgcgcgctg acgccgaaac cgggactggc tttcggttcg gagccggttc agccggtcgt
     5221 acccgacgtc tatgtgcgtg agggcgcgga cggaagctgg catgttgaac tcaattcgga
     5281 cacgttgcct cgagttctgg tgaacgcgcg ctattatgcg caagtaagcc gaagcgcgcg
     5341 caatcgcgac gacaagtcct atctcaccga ttgcatgaac agcgccaatt ggctggtcaa
     5401 gagtctcgat cagcgggcgc gcaccatcgt caaggtggca agcgaaatcg tgcgccaaca
     5461 ggatgcgttt ctgactcatg gcgtgcggca tttgcggccg ctgaatttaa agcttatcgc
     5521 cgatgcgatc ggaatgcacg aatcgaccgt ttcgcgtgtg acgtccaaca aatacatggc
     5581 gacgccgcgc gggcttttcg aactcaaata tttcttcact tcggccatcc aatcggccaa
     5641 tggcggagag gcgcattcgg cggaagccgt gcgcgaccgc atccgcgaat tggtcgaaaa
     5701 ggaagaagac gacgtcttgt cggatgaccg gatcgttacc attttaagcg gtgatggtat
     5761 cgatatcgcg cgccggacgg tggcaaaata tcgtgaagct ctgcgcatcc cctcgtcggt
     5821 cgaacggcgc cgccagcgtc agttggaagt gtccgacgcc ggggcatgac cagcggttga
     5881 cagggcttgg gccccgacct atggtccgcg ccttttcgga cgcgcgcgtt gccgcgggcg
     5941 gtgtaaagcc gggggtccga aggcaaaaat gatgcacatt cgtattgcag gaaaacagat
     6001 cgagatcggc gaggccttgc cgcaacaggt ccgcgaaagg ctttcggaaa cggtggaaaa
     6061 gcacttcgat cgtgacgcca cggccaacgt cacatttacc aaagaacgaa tgggatttcg
     6121 ggcggactgt acagtgcatt tgagttcagg agcatcaatg caagctcacg gcatcgcaga
     6181 gcacgcacac gcagcgttcg gtattgcacg ggagcatctc gaaaagcgcg tgcgcagata
     6241 caagaggcgg cttaagaacc atcacgaccg cggcgggctg agcccttccg cgtcgctgta
     6301 acacgacagg tcatcatgga aatcgcggat ttgctttcgc ccgatgcggt tcttgcgcat
     6361 gtgaaggcta gcaccaagaa gcaggtgctg caggagatgg cgcataaggc cgcgatgctt
     6421 acccagcttc cggagcggcg catcttcgaa attttgaccg agcgcgaaaa gcttggttca
     6481 accggcatgg ggcagggcat cgcgattccg catggccgcg tggcgggcgt agagaagatg
     6541 accggcctgt tcgcgcagct cgatcatccc gttgatttcg attcgatgga cgatcagccc
     6601 gtcgatctgg tgttcctcct gctggctccc gagggcgcgg gtgcggatca tctcaaggca
     6661 ttggcgcgcg tctcgcgcct gctgcgcaat cagacgatct gcgagaagct ccgcgccgca
     6721 ccccaggccg ctacgctcta tgcgcttcta accgagccgt cggccgctca ggcggcgtga
     6781 ctgtctcctc agccgcttcc cgcgagcaga cgcgcaaggc cgccaggctt ctcctcggcg
     6841 ggtttcttgc aatggcggtt gccatgggaa tcggacgttt cttctatacg ccgctgctcc
     6901 cgatcatgca gcgccagttg cgctttggcg ccgacattgc gggactgatc gcgtcggtca
     6961 attttgtcgg ctacctcctg ggcacggtga tggcggcgtt cgttccgaaa ggtcgcacgc
     7021 gccttctcgt gtttcgcttc gcgctgattg caagcgtcgc gaccacgttc gcgactggtc
     7081 ttaccgaatc ggtctcccta tggcttgtcc ttcgcggggc ggctggtgtc gccagcgctt
     7141 ttgtcttcct cttcgccgcg gccatcgtcg ccgaaacgct caccgcgacc ggaaagcccg
     7201 ggcatgtcgg ctggctgttc ggcggtgtgg ggatcggcat cgccttgtcc ggactgatcg
     7261 taagaatcgc gagcggtgtt cttgactggg cgggactctg gatagcggcg ggcgcgctgt
     7321 cggtgctggc cgtgccgttc attttctcgg cggtcagaga catgacgctg gcgccggatc
     7381 cttcgcgcga gaagatcgcg caccgcgtgc cgcgtccgct gccgttgtgg cctcttttgg
     7441 tgaactacac ctgcgaaggc ttcggctatt cgatcatggc gaccttcatc gtggcgatcg
     7501 tgaaggcccg gcccggcatg gaggcggtcg gagattgggt ctgggtggtg gtcgggttgg
     7561 ctggcttgcc cagcacgatg ttctggtcat ggattgccga gcgcatcgga tacgcggtgg
     7621 ctttgacgct cgcctatgtc gcgcaactca tcggcatcct attgcccgtc gtctcggacc
     7681 tcccatcggt agctcttctg gccgcgattc ttttcggcgg aactttcatg gccatcaccg
     7741 cgctcgttct cgcattggga cgcgaatccg caaacggtca agggttcgca gtgctgacag
     7801 cgggttttgg cggggggcag attttaggcc cgctggcggc cggttatctc gtcgcggggc
     7861 gcacggattt ccatcaagca ctgctggtct cggcgggcgt cattctagcc gggctcttct
     7921 ttctcgcgct tgcgattatg caagcgcgag tgcgtgaacg aatggttaat gcacgctgac
     7981 cggcacgaaa ttgttttgca cggccgcact gtaggcagat gcccaattgt cggtaatccc
     8041 aagtgtccgg ccgtcggccg cgtgcagcgc atacagcttt acattggccg gcagcttgat
     8101 ttccgacgga attaccaacc ccatggcctt ggcttcatcc agagcaatag gcttgatata
     8161 ggccaaacgc gctccttcaa aggccgggcc ttcgagctct tcagtttcgg gtgagtcgtc
     8221 gtcaaagatt tcttcttctt gtctcatgac actctcctgt tcaggtccga ttgttaccat
     8281 tgctgctgtg aacatcagct gaatgaatag gtatcgtccg tacaacgctt tcctggtgcg
     8341 gacgctccaa ttcgattgtg agcaggccat ggcggagttc ggcactcagc acttcgattc
     8401 gctcggcaag gacaaagttg cgctggaact gacggctcgc aatcccgcga tgtaggaagg
     8461 tcttgtcctc ggggtccgtc atgcgcccac ggatgacaag ctgattgtcc tccatggtca
     8521 cgctgagatc ttcggctctg aagccggcga cggcgagcgt gattttcagc cggttttcgg
     8581 tgatgtgctc gatgttgtaa ggcggatacg agtccgcttt tgcggtgcgc tcaagcagct
     8641 tttcgatatg ttcaaaaccc aacagaaacg ggttgttgaa gaaggtccga ttcatcaccc
     8701 aaagccctcc ttggcggctt tgtccggccc ccgacctggg cggcctgcac tgaaaacggc
     8761 cccacatgag caccgttgct aaaagaaatg taggaattgt ctggaaccaa tcaagatgtt
     8821 ggcctggctc ttgccgtcag ggtctctccg gggcaggaag ggattatgcg cgcgcccttc
     8881 gttttattgg acgattcccg gtccgagcag cagcgctcgc ggcttttcac gggcttggtc
     8941 cgcacagtgg aggccaggac agcaagcgaa gtccagcccg ctctggacgc cctggccgcg
     9001 gcccgcaagg agggcctgtt cgcggccgga tatttcgcct acgagctcgg ttatgcgctg
     9061 gaatcgcggc ttgtcccatt gctgccgcaa tcctctatcc ctctgctctg gttcggcctc
     9121 ttccactcac aggaaaccct tgcgggtccc gaaaccgatc gctggctcgc gcgacaatgt
     9181 cgaggccgcg cctatgccgg cccgttgcat ccgggcgaca ccgagaaaga gtatgccggg
     9241 cgtttcgcac gcgtccgcga tgacatcctg gccggtgacg cctaccaggt gaatctgacc
     9301 tttgcctgcc gcttcgcatt cgcgggcgat gcgctggcgc tctatgctag gctacgccgg
     9361 caagcgaagg ccggtcatgg ggcctatgtg gacgatggca cgcgaagcat cctcagcctg
     9421 tcgccggaac gatttttcgc cgttgcggac ggagttctga cggcgcggcc gatgaaggga
     9481 accgcaccca ggggtaagga cgcagcgagc gatgcacgca tcgccgccgg gctccagacg
     9541 agcgaaaaag accgcgccga aaatctcatg atcgtcgatc tgatccgcaa cgacatcgga
     9601 cgcgtcgcca aaaccggtac cgtcaaggtc gaggatcttt tcgcggtcga gacctatccc
     9661 accgtgcatc agatggtctc gacggtgcgg ggcgaattgc gtgccggcgt gacgccggcc
     9721 gatctggtgc gcgcgctctt tccatgcggc tcggtgacgg gcgcgccgaa attgcgtgcc
     9781 atggagatca tcgcagaact cgaacgcacg ccgcgcggcc tttattgcgg cgctatcgga
     9841 gtgttcgcgc ccgacggcgg cagcgatttc aacgtggcga tccgtacatt gacgatagag
     9901 gggcgcgagg gacggctcgg catcggcggc ggtatcgtcg ccgattcgaa accggatgcg
     9961 gaatatgagg aatgcctgat caaggcgcgc ttcttcgggg agggacgtcc gcctttgtcg
    10021 ctgatcgaga cgctgcgcta cgagcccagg caaggattcg tccgcgagcc tctgcatttg
    10081 gcgcggctgg cacgttcggc ggccactttc ggattctctt tcgacgaaaa gaccgcgcgc
    10141 aatgtccttc gcgattgcgt cacgcacacc accgcatctt tgcgcgtgcg cctgcaactg
    10201 gatgaagacg gttccttcac aacgagcatc gctgctttcg tgccggctgc ggccggaacc
    10261 gtctggacct acaagatttc ggcccatcag gtgcaaagtg cagatgttct ctcccgtcac
    10321 aagacaaact ggcgcacgct ctatgacgaa gaacgcgcgg cagcaaacac ttcgggctgc
    10381 gacgaagtgc ttttccgcaa tgagagagac gagatcgttg aaggcagcac aaccaatcta
    10441 ttcgtgcgca tcggcgatcg gctggtgacg cccgttcttt cctgcgggcc gctggaagga
    10501 tgtcttcggc gagcgcttct ggatgcgggc gaatgcgtcg aaggcatcat tcttgcctcc
    10561 gatctggaaa agaacgaggt ctatctcgga aattcgctgc gcggattgat aggggcgcga
    10621 tcctttaatg gatgagctta cgcgaaaagg tccagaccgg ttcgcgtaag catataggcg
    10681 gcgactgcga acaccgctcc ggcgaatata taattcagcg cctgtttctt tgccgccagc
    10741 cgtatcgccg cacgcattcc gaagacaccg ccaatcgcgc ctcccaggat gaactcggtt
    10801 gccgtacccc aatcgataaa gccagaaagc gcataattca ccgctgttgt gaggccgaag
    10861 gttccaaccg cgaacaagga cgatccgatg gcgttcagga gagggaggcc ccctccgtac
    10921 ataatacccg ggacgatcat gaatccgccg ccgatgccga agaagccgga gattgttccg
    10981 gcggcaagcc cgacaatcac caggcgaaat acgattcggc ttgtcatccg ccggcaaatt
    11041 tcaccgccgc cgttgcgcgg tcgcaggatg gcgattccgg ccgcgatcat caccatggcg
    11101 aacaggaaaa gaagatgttc accggacaca agctttccca aggtggagcc gatgactgcg
    11161 ccgagcatac ccgccgatcc aaacagaatc gcgcaaggcc aatgcacgtt gcccgcccgc
    11221 caatggctga gaagattgat gaaggcattc acggcaaccg caagggcgct cgttccgatg
    11281 gcgatatgag cgtcctggac accgacgaca taaagcaaca gaggaaccgc caatatcgat
    11341 ccgccaccgc ctatgattcc gagtgaaaat ccgaccgcgc ctcccgacaa caatgcgcca
    11401 agggtctgca acgatatcaa tgttgcgcct tggaagcggc ccgttgtgca tcgatgatcg
    11461 gttgaaacgg tcccagacgg tgctgctcgg gggggaattt atcggtgaac ggcccaaagg
    11521 gcgcatcggc gggtttgcgc gcgcgatcgg gataatcggc gtcgagattg gcgcgtttgg
    11581 ggcgcggctg gctgttgatg taggccgcga cgtcccaggc atcttccacg gacaattgcg
    11641 gcgcatcgta atgggtgccg aacggcatgt tggcgtggat gaagcttgcc gagccgatca
    11701 gccgatgcat gccggcgccg tcgttgaaac tgtccggtcc ccagagcgga ggatagagat
    11761 agccctttgc ttcaccgggc ggccctttgc gttgaccttg gccgtccgct tggtggcaga
    11821 acgcgcattc tttttcgtag atctgggaac cgtgcttggg atcggcggcg cgactgagca
    11881 gcggcagact cggcgaggcg cggccttcga gggattttcc gaccggcacg ttcgtgctga
    11941 gaaactggat ataggcaagg agcgctttca tctctttgcc gccaaggggg agtgcgcggc
    12001 cattcatgct gcgctccata cagccctcga tacgttcttc gagcgtgcgc acttcgtttt
    12061 cacgcgcgat atacacggga aacactcccc aaatgccgat catcggaatc gcgaatttct
    12121 gcgtgccgcc tttgagatga cagctctggc aggagagatt gttgccggcg tagcgcatgg
    12181 aggtatccgg cacctctgga ccgatgacac tgtaggtttc attgaagaca cgctgtccat
    12241 agacgacgat gtctttcccg gcacccggca caagcgaatc aggattgggc tgaggccata
    12301 cctcggtcgt ttggcctagc gcctcgggca taccgaacag cgccaatgca aaactgaaga
    12361 taattgcggt tcgacgtccc atgccatcct ccaaaaccaa agccatcata aggtggagcg
    12421 cggtcaattt ccacttcagg cttgtggaat gcgtggcgcg cttctaattt gccagggcca
    12481 atcacaaggg gagagggaaa tgttcaaaag acttcgcgcc gcggtggcgc tcatggcagt
    12541 ggcgataccc gttttcctgg ccggcatgca cacggcatcg tcacaaaata cgcccaagct
    12601 gcacaaaatg gttatccagg tgtcgcgcga agataccgaa ggcatgatgc tcgcgctcgg
    12661 caatgcgatc aatgcgaaaa agctttacga cgcgcgcggc gagcagttcc aagtcgagat
    12721 cgtcgcttat ggtcccggca tcaccatgtt ccgcgaggac aaatcgccgg tcaaagaacg
    12781 tctcgcagaa gcgaaaaaag aaatcccggg cattgtctac agcatgtgcg gcaacgccaa
    12841 ggccgcagcg gaaaagcgtg agggccacga ggtcgttccg atagctggag tgggcgtcgt
    12901 gcccgcgggc atcgtgcgcg tcgccgagct gcaggaacaa ggctacgtct acattcgtcc
    12961 ctgatatatt ggttcggaga taaagatggc gttcatttcc aggcggcgct ttgctttggg
    13021 tgcaggcgcc gccgcgacaa cggctttggt gggcccgcgc cgtgggcgcg ccgcagcgcc
    13081 tattgaaatg cgccaattcc acaatcagac cgaggccacg cccttggaca aacggctcaa
    13141 ggagatgtgg gctgcggtcg aaatggaaac cggcggcaag attcgcgtca aaacctatgc
    13201 ccgcaacgac aatattc
//
LOCUS       DASYEG010000299        10451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4465195, whole genome shotgun sequence.
ACCESSION   DASYEG010000299 DASYEG010000000
VERSION     DASYEG010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 10451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10451
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4465195"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VGM72_12690"
     CDS             <1..306
                     /locus_tag="VGM72_12690"
                     /inference="COORDINATES: protein motif:HMM:NF016189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
                     /protein_id="HEY4266170.1"
                     /translation="VMFASTLLGGLVFGRLFIKLMLGQTLNLPEDAWRSLTWRWAFFF
                     MALAILNEIVWRNVSTNAWVAFKLWGVFPLTLLFAMAQTPFIARHQIESDETPAASR"
     gene            549..1058
                     /locus_tag="VGM72_12695"
     CDS             549..1058
                     /locus_tag="VGM72_12695"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY4266171.1"
                     /translation="MASKGPRKTNGGFDLSQSPSHLLRRCVQYANDLFSREPGASDLT
                     KQQFTVLTAVEQNEGMSQTELVTITGIDRSTLAEMIRRMIEKGLLDRERTESDQRANA
                     VRIAAAGRKALRSARMASERVERTLLSSLAAADRTKFLKMLTSVVGQAETENPNPRTE
                     ARAKTRRSR"
     gene            complement(1315..1854)
                     /locus_tag="VGM72_12700"
     CDS             complement(1315..1854)
                     /locus_tag="VGM72_12700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEY4266172.1"
                     /translation="MKRLYFVIPVVIFLSLAALLYIGLFQGPPSELPSPLIGKPAPGI
                     ALPALDAQTPGFARSELGQGHPIIVNFWSSWCAPCRVEHPTLEALAKQPGVTIYGVDY
                     KESLYGRDAKDARAFLTELGNPFEKINQDSEGRVAIDWGVTGVPETFVVDSKGIIRVH
                     YAGPLNDEVLQRLILPALK"
     gene            complement(1851..2027)
                     /gene="ccmD"
                     /locus_tag="VGM72_12705"
     CDS             complement(1851..2027)
                     /gene="ccmD"
                     /locus_tag="VGM72_12705"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEY4266173.1"
                     /translation="MASFFAMGGYAVYVWPAYIISALVLTAVVVVSLQAHARAKRNVR
                     QLEDDNGDGAESQR"
     gene            complement(2031..2756)
                     /locus_tag="VGM72_12710"
     CDS             complement(2031..2756)
                     /locus_tag="VGM72_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003079809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HEY4266174.1"
                     /translation="MMITFLANPKRFLDFSNAIAPWVCALAVVLLGTGFYWALLATPP
                     DYQQGQSVKIMFVHVPAAYMGIMIYGVMAGASLVGLVFRHPLADVAAKSAAPLGAAFT
                     ALALLTGSLWGRPMWGTYWQWGDARLVSELILLFIYLGYIALWNAIDDPARAAKAAAI
                     LSLVGAADVPIIHFSVDWWNTLHQPASIIRPGGPTIDKSFLMPLFVMMLGYTMLFAWL
                     LLIRMQTEILERRARSLMMAKGA"
     gene            complement(2794..3459)
                     /gene="ccmB"
                     /locus_tag="VGM72_12715"
     CDS             complement(2794..3459)
                     /gene="ccmB"
                     /locus_tag="VGM72_12715"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020043820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEY4266175.1"
                     /translation="MTAFQALLVRDMRLAFRAGGGASLAAAFFALVSTLVPFGVGADL
                     VLLGRIAGGVLWVSAALAALLSLDRLFQADFEDGSLDVLSLSPLNLEWAATAKILAHW
                     LTTGLPLVVLSPVLGLLLNLPAPGYLALVGSLIIGTPALSGVGAIGAALTLSIRRGGL
                     ILALIVLPLITPALIFGAGAVTDALDGTGRGAFLFLAAFSVAAVALSPFAAAAGVRLN
                     LGG"
     gene            complement(3456..4079)
                     /gene="ccmA"
                     /locus_tag="VGM72_12720"
     CDS             complement(3456..4079)
                     /gene="ccmA"
                     /locus_tag="VGM72_12720"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEY4266176.1"
                     /translation="MAVLSLVEVQNLTCERGERRIFRDIDFAVATGQVLTLEGPNGAG
                     KSSALRILAGLLPPTAGNIRMQMSAGYSVTDSEERGKLTGWIGHNDGVKAQLTVVENA
                     KFFAALYGARIDVAGVLDHVGLKRLSGVPSQYLSAGQRKRLGLARLILSNRPLWLLDE
                     PLSSLDAAGRRLAAQLIGEHCDDGGIAIVATHETLGLDATRFFLGTA"
     gene            complement(4218..4940)
                     /gene="phbB"
                     /locus_tag="VGM72_12725"
     CDS             complement(4218..4940)
                     /gene="phbB"
                     /locus_tag="VGM72_12725"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008892266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEY4266177.1"
                     /translation="MARIAIVTGGTRGIGRAISIALKDAGCTVVANYASDEARAQSFA
                     AETGIAAKKWDVSDFDACLTAVDMIAAEFGVVDIVVNNAGITRDGTMRKMSRTNWDQV
                     LDTNLGGCFNMCKAVWEGMLAKNFGRIVNIGSINGQAGQYGQVNYAAAKSGIHGFTKA
                     LAQEGAAKGITVNAIAPGYIDTDMVSAVPANVLEKIVARIPVGRLGKADEIAHGVVFL
                     VGDGAGFVTGSTLSINGGQHMY"
     gene            complement(5000..6172)
                     /locus_tag="VGM72_12730"
     CDS             complement(5000..6172)
                     /locus_tag="VGM72_12730"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY4266178.1"
                     /translation="MTDVVIASGARTPVGSFTGAFANVPAHDLGTAAIKAALERANVA
                     PREVSEVILGQILTAGQGQNPGRQASMKAGIPVETPAYLVNQLCGSGLRAVALGYQSI
                     RNGDASIIVAGGQESMSMAQHAAHLRNGQKMGPLEFADTMIKDGLMDAFHGIHMGITA
                     ENVAREFQITREQQDRFAVASQNKAEAARKAGRFKDEIVPVTVTERKGVKIVENDEYI
                     RDGVTYESISALRPAFAKDGTVTAANASGLNDGAAALVLMSAKEADARGIAPLARVVS
                     WASAGVDPKIMGTGPIPASRMALKKAGWNASDLDLIESNEAFAAQACAVNNGLGWDLA
                     KVNVNGGAIAIGHPIGASGGRILVTLLYEMGRRNAKKGIATMCIGGGMGIAVCVER"
     gene            6356..7009
                     /gene="phaR"
                     /locus_tag="VGM72_12735"
     CDS             6356..7009
                     /gene="phaR"
                     /locus_tag="VGM72_12735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEY4266179.1"
                     /translation="MSEKESGAPGPIIIKKYANRRLYNTQTSSYVTLDHLCEMVKDGV
                     EFEVRDARTGEDITRQVLAQIIFDEENKGQHLLPIQFLRQLIRFYGDSLQSFVPSYLE
                     MSMESFTKNQQEMREKFAEALGGKLGFKDFEQMTRQNIQMFERAMKMFTPFSGSMGTW
                     GDKEVPNPFAAFGGMGAKPSPAAEPRPSSPPREDEIRELKDQVAAMRQQIADLAQKK"
     gene            complement(7091..8839)
                     /locus_tag="VGM72_12740"
     CDS             complement(7091..8839)
                     /locus_tag="VGM72_12740"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEY4266180.1"
                     /translation="MGVLVQNHAIQLAFKDRLEALGQTADVTRLRLALLGARAVAFDW
                     TLADDRIRWDGTEAAIPEFSELERLETGQGFRAWLSLAARTRLLEIIEEPASYDSTFR
                     MEFQAVTKTSRQWFELSAVCIPGPGGHSERITGMVREITENRNALTRLSYLATSDELT
                     GHLNRARLRDELTRVIGRSTADGRSCAFIVTAIDKLAVINETYGFDAADEVIVAAGQR
                     LAQALRTSDIIGRIAGNKFGVVLGECGEREMSLIAERLHTAVRSEPIPTRAGAISASV
                     SVGAVWLPQGAATSQDVMLRAEEALERAKAMGRNGFAVYSGSAQRESQRRRLINIGDE
                     IMSALGEQRLVLAYQPIVGARSRQPEHYECLLRMQRKDGTVAAAGEFIPAAETLGLVR
                     HADRRALEIAVAQLYRHPHIKLSINVSGTTAGDHSWLQSFINYVRENREVAERMTVEL
                     TETAALQAFEENARFVTRLRDMGCRVAIDDFGAGYTSFRNLHNLRVDMVKIDGEYVKN
                     LSGSPDNQLFVRTLVDLAKNFQLETVAEWVGSEQDAELLVDFGVDYFQGYYFGEPELS
                     PAWLEPNERLAHSAFE"
     gene            8965..9306
                     /locus_tag="VGM72_12745"
     CDS             8965..9306
                     /locus_tag="VGM72_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266181.1"
                     /translation="MKIGGNNSDFRARRALRAPRIVEKPDSVSRLSEENLRETAQTAI
                     RTERRSVARASRFGTYYAGGVDRRGMQARFVAQVLGQILDSGDGIALIARRAYAQSSR
                     ERKETRLVRVL"
     gene            complement(9311..10108)
                     /locus_tag="VGM72_12750"
     CDS             complement(9311..10108)
                     /locus_tag="VGM72_12750"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HEY4266182.1"
                     /translation="MHARFFLLSGILAAALSPSAWAGQPAAASRQTPVAAATRPIVVE
                     LFTSQGCSDCPAADRLVTELAKRKDVIALSLPITYWDMLGWKDTYATDANTQRQKSYA
                     RAMSHSGIYTPQMIVDGRFDVVGNQRDRVMAALAARTNDGLGETSIPITVGAVSGRLE
                     IAIPAAKTKTKPLATIWVMRTLNQGTVNVEQGENRNHSLTYTNVVRDLQRAGEWNGEA
                     MKIDLPLNLANAKHDGIAVILQAHDYGPVLGAASLQLQAGEHVAAPR"
     gene            complement(10195..>10451)
                     /locus_tag="VGM72_12755"
     CDS             complement(10195..>10451)
                     /locus_tag="VGM72_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266183.1"
                     /translation="GILPLEFAGGKTRNDYALTGKEAIDIGVREGASGTRKKVTARVT
                     YPDGRTVDMPLVARIDTVDEVAYFKSGGILQHVLRYLAAA"
BASE COUNT         2135 a         3255 c         3187 g         1874 t
ORIGIN      
        1 gtcatgttcg cgagcacatt attaggcggg ctcgtattcg gacggctgtt cattaaattg
       61 atgctgggac agacgctgaa tcttcctgag gacgcatggc gctcgctcac atggcgctgg
      121 gcgtttttct tcatggctct cgcaatcctc aatgagattg tatggcgaaa tgtctcgacc
      181 aatgcatggg ttgcgttcaa gctgtggggg gtatttccgt tgacgctgct tttcgcgatg
      241 gcgcagacgc cgttcattgc gcgccatcag atcgaaagcg atgagacgcc cgccgcttcg
      301 cggtaaacgg ttgtagagct tttcgacatt aaaccggcct ctgctggagg gcacgagccc
      361 acgcaccttt cgcgtcgcga aacgcggcac tcgcatcgat acagcaagtg gaactctcat
      421 gcatcattcg atcaaaatca ctcaaagacc gcttgcgtcc acgatgcgtt gcgctaaggt
      481 tgccggccag taccgacggg tacggacgat tttcactgaa atctcgaaat tgcaccgggg
      541 gttgggggat ggcttcgaag ggtccgcgca agaccaatgg cggttttgac ctttcacagt
      601 ccccgtcgca tcttctgaga cgctgcgtgc aatatgcgaa cgatctgttt tcgcgcgagc
      661 cgggcgcgag cgatctgacc aaacaacaat tcacggtgct taccgcggtc gaacagaatg
      721 agggtatgag ccagacggaa ctcgttacca tcacaggcat cgaccgttcc acacttgcgg
      781 agatgatccg gcggatgatc gagaagggtc tcctggaccg cgaacgcacc gaaagcgatc
      841 aacgcgcgaa cgcggtgcgc atcgcggcgg ccggcaggaa agccctgcgc agcgcacgca
      901 tggcaagcga gcgggtggaa cgtaccttgc tgtccagtct cgccgccgcc gaccggacaa
      961 aattcctgaa aatgctgacg tcggtcgtag gtcaggctga aaccgaaaat ccgaacccgc
     1021 gcaccgaagc gcgcgcgaaa acccggcggt cacgctagaa tcgcagcctt tagaccttaa
     1081 tatccagctg ggttcccggg cgcggaaccg cacctggttg aaccggttgc ctgcgcgttt
     1141 cctccgtgga agaggccgaa aaatcgagcg gttcgaacgc ggtcgtttga gccggcgctt
     1201 tgatggcgga cggacgttgg gtctgtgcct gtgcggcaag gagaaaattg gagacgtcga
     1261 tccgcatcgg tccaccatga cccaacatgg tttgcaaaat ctaaatgcca cgcatcattt
     1321 cagcgccggc aggatcaatc tttggagcac ctcgtcgttc aacggccccg cgtaatgcac
     1381 gcggatgatg cctttgctgt cgaccacaaa ggtttccggc actccggtga cgccccaatc
     1441 gatcgcgacg cggccctcgc tatcctgatt gatcttttcg aacggattgc cgagctcggt
     1501 cagaaaggcc cgggcatcct tggcatcgcg tccatacagc gattccttgt aatcgacccc
     1561 gtagatcgtg acacccggct gtttggcgag cgcctcgagt gtcggatgtt ccacccggca
     1621 tggggcgcac cacgacgacc agaaattgac gatgatggga tgaccctgcc caagctcgct
     1681 gcgggcgaaa cccggcgtct gcgcatcgag cgcgggcagc gcgatgccgg gcgcgggctt
     1741 accgatcaga ggcgaaggaa gttcggacgg cggcccttgg aacaggccga tataaagcag
     1801 ggccgcgagg ctcaaaaaaa tcacaacagg gatgacgaaa tacagtcgct tcatctttgg
     1861 ctctctgcac cgtccccgtt atcatcctcc aactggcgca cgtttctttt tgcgcgcgca
     1921 tgcgcctgca gggaaaccac cacaacggcg gtcagcacca gcgcgctgat aatataggca
     1981 ggccacacat agaccgcata gccgcccatg gcgaagaaac tcgccatgat ttacgcgccc
     2041 ttcgccatca tcagcgagcg cgcgcgccgc tcgagaatct ccgtttgcat gcggatcagc
     2101 aagagccagg cgaacagcat cgtatacccg agcatcatga cgaagagcgg catgaggaag
     2161 cttttgtcga tggtgggccc acccggacgg atgatgcttg ccggctgatg cagcgtgttc
     2221 caccaatcca ccgaaaaatg gatgatcggc acatcggcag cgccgaccag cgacaggatg
     2281 gcagcggcct ttgccgcgcg cgccggatcg tcgattgcat tccacagcgc gatgtagccc
     2341 aggtagatga agagcagaat caactcggag accagccgcg catcgcccca ttgccaatag
     2401 gtgccccaca tcggccggcc ccagagcgaa cccgtgagca gtgccagcgc cgtgaaggcc
     2461 gcgcccaaag gtgctgccga tttcgccgcg acatcggcaa gcgggtggcg aaagaccaga
     2521 ccgaccagcg aggcgcccgc catcacccca tagatcatga tgcccatata ggcggcgggc
     2581 acatgcacga acatgatctt gacgctctgg ccctgttggt agtcgggcgg cgtcgcgagc
     2641 agcgcccaat agaagccggt gccgagcaga accaccgcaa gggcacagac ccagggcgca
     2701 atggcattgg aaaaatcgag gaagcgtttg ggattggcaa gaaaggtaat catcatgacg
     2761 cctacataag ccctccctgc gaaattgtcg aaatcagccg ccgagattga gccgcacccc
     2821 ggctgccgcg gcaaagggcg aaagcgccac cgccgccacc gaaaatgcgg cgaggaacag
     2881 gaatgcaccc cggccggtcc catcgagcgc atcggtcacc gcgcccgcgc caaagatcag
     2941 cgcgggcgtg atcagcggca gcacgataag cgcgaggatc aatcccccgc ggcggatcga
     3001 aagggtcagg gccgctccaa tggcgccaac cccggaaagt gcgggcgtgc cgatgatcag
     3061 cgagccgacg agcgcgagat agcccggcgc cggaagattg agcagaagcc ccagcaccgg
     3121 cgaaagcacg accagcggca agcccgtggt caaccaatgc gccaagatct tggccgtggc
     3181 cgcccattcc agattcagcg gcgacagcga cagcacatcc aggctgccgt cttcgaaatc
     3241 ggcctggaac agccggtcaa gcgagagcag cgccgccagc gccgccgaca cccacagcac
     3301 cccacccgcg atgcgtccga ggagtacgag atcggccccc acgccgaaag ggaccagcgt
     3361 cgaaaccagc gcgaaaaagg ccgcggccag actggccccg ccgccggcgc ggaaggcaag
     3421 ccgcatatcg cgtaccagaa gggcctggaa tgcggtcatg cggtgcccag aaaaaaacgg
     3481 gtggcatcca gacccagcgt ctcgtgggtt gcgacaatgg cgatgccgcc atcgtcgcaa
     3541 tgttcgccga tgagctgcgc ggcaagccgc cgtcctgccg catcgagcga ggaaagcggc
     3601 tcgtccagca gccatagggg ccggttggac aggatcaggc gcgccaaacc cagacgcttg
     3661 cgctgaccgg cggatagata ttgtgacgga acgccggaaa gccgcttcaa ccccacatgg
     3721 tcgagcacac ccgcgacatc gatcctcgca ccatagagag cggcgaagaa tttcgcgttc
     3781 tcgaccaccg tgagctgggc cttgaccccg tcattatgcc cgatccagcc ggtgagcttg
     3841 ccccgttctt cggaatccgt aacggaatag cccgcgctca tctgcatacg gatattaccg
     3901 gccgtcggcg gcaggagccc ggcgaggatc cgcaaggccg agcttttgcc ggccccgtta
     3961 ggaccttcga gtgtcagcac ctgcccggtc gcgacggcaa aatcgatatc gcggaaaata
     4021 cgccgctcgc cgcgctcgca ggtgaggttt tgcacttcga cgagagatag gacagccatg
     4081 accgccgaga ggaatgcttt ggttcttata tgtcatggcc cgcaacggcg ggccatgagg
     4141 agggacgatg acgttcggcg aatggacatg cgccggctgg gcgaccgggt caagcctgcc
     4201 cgcaatgacc ggccgcttca atacatatgc tgaccgccgt tgatggagag ggtcgagcca
     4261 gtgacgaagc cggcgccgtc gccgaccagg aaaaccaccc cgtgggcgat ctcatccgcc
     4321 ttgcccaggc ggcctaccgg aatgcgcgcg acgatcttct ccagcacgtt cgccggcacc
     4381 gccgagacca tgtcggtgtc gatgtagccc ggcgcgatcg cattcacggt gatccccttg
     4441 gcggcgcctt cctgggcgag cgccttggtg aagccatgga tgcccgattt cgcggccgcg
     4501 taattgacct ggccatattg gccggcctgg ccgttgatcg agccgatatt gacgatgcgg
     4561 ccgaaattct tcgccagcat cccctcccag acggctttgc acatattgaa gcagccgccg
     4621 agattggtgt cgagcacctg atcccaattg gtgcggctca tcttgcgcat ggtgccgtcg
     4681 cgggtgatgc cggcattgtt gacgacgatg tccacgacgc cgaattcggc cgcgatcatg
     4741 tccaccgcgg tcaggcaggc gtcgaaatcg gacacgtccc atttcttcgc ggcgatgccg
     4801 gtctcagccg cgaagctttg cgccctggcc tcatcgctgg cgtaattcgc aaccacggta
     4861 cagcccgcgt ccttaagggc aatcgagatc gcccggccga tcccgcgggt tcctccggtg
     4921 acaatcgcaa tgcgtgccat gattcctccc tacgtctcag gccgagcatt tcgaagcatg
     4981 ggatgaaaat cgcccgtgtt caacgttcca cgcacacagc gatgcccatg ccgccaccga
     5041 tgcacatggt ggcaatgcct ttcttcgcat tccggcgtcc catctcatag aggagcgtga
     5101 cgagaatgcg tccgcccgag gcgccaatgg ggtgtccgat cgcgatggcg ccgccattca
     5161 cgttgacttt cgcaaggtcc cagccgagcc cattgttcac ggcgcaagcc tgggccgcga
     5221 aagcctcgtt ggattcgatc aaatcgagat cggacgcatt ccagccggcc ttcttcaatg
     5281 ccatgcgcga ggcgggaatg ggtccggtgc ccatgatctt ggggtcgacc ccggcgctcg
     5341 cccaggaaac gacgcgggcg aggggcgcaa tgccacgggc atcggcctct ttggcgctca
     5401 tcagcacgag cgcggcggcg ccatcgttca ggcccgacgc attggccgcc gtcaccgtcc
     5461 cgtccttggc gaaggcagga cgcagcgcgg agatggattc ataggtcacc ccgtcgcgga
     5521 tatattcgtc gttctcgacg attttcacgc ctttgcgctc ggttacggta accggaacga
     5581 tctcgtcctt gaagcgcccc gccttgcgcg cggcttccgc cttgttctga gacgccaccg
     5641 cgaagcggtc ctgctgttcg cgcgtgatct ggaattcgcg tgccacgttc tcagcggtaa
     5701 ttcccatatg gatgccgtgg aacgcatcca tcaggccgtc cttgatcatg gtgtcggcga
     5761 attccagggg gcccatcttc tggccgttgc gcaaatgcgc cgcatgctgc gccatgctca
     5821 tggattcctg accgccggcc acaatgatgg atgcatcccc gttccgaatc gactggtagc
     5881 caagcgccac cgcccgcaag cccgagccgc acaattggtt caccagatag gccggcgtct
     5941 cgaccgggat gccggccttc atggacgctt ggcgcccggg attctgcccc tgccccgcgg
     6001 tcaggatctg gcccaggatc acctcggaga cctctctggg cgcgacgttc gcgcgttcca
     6061 gagccgcctt gatggcggcg gtccccagat catgggcggg cacattggcg aacgccccgg
     6121 taaaggaacc gaccggggtg cgggctcccg aagcaatgac gacgtcggtc atggaaaagg
     6181 cctttgctgc gagccaccgc cattcatgcg ccggtgggca ggttgcgtaa accaaaaatc
     6241 aaaaaatgct gcaatgcggt aaagagtcct ggacagggcc gccccatcgg tgctgtgtta
     6301 cagacacgag ggaaccaagg ggcggcaaag cccactatat cgattgagga accgcttgag
     6361 cgaaaaggaa agcggggcgc ccggtccgat catcataaaa aaatacgcga accggcgtct
     6421 ctacaacacc cagacatcga gttatgtgac gctcgatcat ctctgcgaga tggtgaagga
     6481 cggtgtggaa ttcgaggtgc gcgatgcgcg caccggcgaa gacatcaccc gtcaggtgct
     6541 ggcgcagatc atcttcgatg aggagaacaa ggggcagcat ctcctgccga tccagttcct
     6601 gcgtcagctc atccgatttt acggcgacag ccttcaatcc tttgtcccct cctatctcga
     6661 aatgagcatg gagagcttca ccaagaacca gcaggagatg cgggagaaat tcgccgaagc
     6721 gttgggcggc aagctcggct tcaaggattt cgagcagatg acgcgtcaga acatccagat
     6781 gttcgagcgc gccatgaaga tgttcacgcc gttcagcggt agcatgggga catggggcga
     6841 caaggaagtc cccaatccct tcgcggcatt cggcggcatg ggggccaaac cttcgcctgc
     6901 ggccgaaccg cgcccgtctt cgccgccgcg cgaagatgaa attcgcgaac tcaaggatca
     6961 ggtcgcggcc atgcgccagc aaatcgccga ccttgcgcag aagaaataga gcggtagcga
     7021 ttttaccgaa ctgaaatcaa aatcgcgtcg ccgccgggcc tgtccccggc ccaatacggc
     7081 gcgccagggg ctattcgaac gcggagtgcg ccaggcgctc gttcggctcc agccaggccg
     7141 gcgagagctc aggctcgccg aaataataac cctggaaata atcgacgccg aaatcgacga
     7201 gcaattccgc gtcctgttcc gaacccaccc attcggcgac cgtttccagt tggaaattct
     7261 tggcgagatc gaccagcgtg cggacgaaga gctgattgtc cggcgagccg ctcaggtttt
     7321 tgacatattc gccatcgatc ttgaccatat cgacgcgaag attgtgcaga ttgcggaagg
     7381 acgtgtaacc ggcgccgaaa tcatcgatcg cgacacggca gcccatgtcg cgcagccgcg
     7441 tcacgaagcg cgcattctcc tcaaaagcct gcaaggccgc ggtttcggtc agctccaccg
     7501 tcatgcgctc ggccacttcg cgattttccc gcacgtaatt gatgaaggat tgcagccagg
     7561 agtgatcgcc cgccgtcgtc cccgacacat tgatggagag cttgatgtgc ggatgccgat
     7621 aaagctgcgc gacggcgatt tcgagcgccc ggcggtcggc atggcgcacg agcccgagcg
     7681 tctcggccgc cggaatgaac tcgcctgcgg cggccaccgt gccgtccttg cgctgcatcc
     7741 tgagcaggca ttcgtaatgt tcgggctgac gcgaacgcgc acccacgatc ggctgataag
     7801 cgagaaccag gcgctgctcg ccgagcgcgg acatgatctc gtcgccgatg ttgataaggc
     7861 ggcggcgctg ggattcgcgc tgcgccgatc ccgaatagac ggcaaaaccg ttgcgcccca
     7921 tcgctttggc gcgttcaagc gcttcttcgg cacgcagcat gacgtcctgg ctggtggccg
     7981 cgccttgggg cagccatacc gcgccgaccg acacgctcgc cgatatcgcg cccgcccgtg
     8041 tgggaatcgg ttcgctgcgc accgcggtgt gcaggcgctc ggcgatcagt gacatttcgc
     8101 gctcgccgca ttcgcccagg acgaccccga atttgttgcc ggcgatacgc ccgatgatat
     8161 cggaggtgcg caacgcctgc gccaaacgct ggcccgccgc cacgatcacc tcatcggctg
     8221 catcgaagcc gtaggtttcg ttgatcacgg cgagcttgtc gatcgcggtc acgatgaacg
     8281 cgcaggaccg tccgtctgcg gtggaacggc cgatgacgcg tgtcagctca tcgcgcagcc
     8341 gggcacggtt cagatgtccc gtcagttcgt cgctggtggc gagataggaa agccgggtga
     8401 gcgcgtttct gttttccgtg atctcgcgca ccatacccgt gatgcgttcg gagtgaccgc
     8461 cgggccccgg aatgcagacg gcggaaagtt cgaaccattg acgcgaggtc ttggtgacgg
     8521 cttggaactc catacggaac gtggaatcgt aggacgccgg ttcctcgatg atctcgagca
     8581 gccgcgtgcg cgccgccaga gaaagccagg cgcgaaagcc ctgacccgtt tcaagacgct
     8641 cgagctcgga gaattcgggt atcgccgcct ccgtcccatc ccagcggatg cgatcgtcgg
     8701 caagcgtcca atcgaaggcc accgcgcggg cgcccagcaa agccagccgc agacgcgtga
     8761 cgtccgcagt ctgaccgagt gcttccaaac gatccttgaa tgccagctga atggcgtgat
     8821 tctgcaccaa tacacccata atttcgcgat cttcgtcgcg ccttgaaccg caggctagcg
     8881 ttcggtgcgt ttaagaaagc tgaaattttt cgggcttgcg cgaacgagtc gccaaacggc
     8941 ctgagatttg cttaagtatg ggttatgaaa ataggcggca ataattccga ttttcgtgca
     9001 cgacgcgcgc tccgcgcgcc gcgtattgtc gaaaagccgg atagcgtaag ccgcttaagc
     9061 gaagaaaatc tgcgcgaaac cgcgcaaacc gcgattcgta ccgaacgccg cagcgtcgcg
     9121 cgcgcgtccc gttttgggac atattacgcc gggggtgtgg atcggcgcgg gatgcaagcg
     9181 cgcttcgtcg cccaggtgct cggtcaaatc ctggattcgg gggacgggat cgcactgatt
     9241 gccagacgcg cttatgcgca gagcagccgg gagcgcaagg agacacgtct cgtacgagtg
     9301 ttgtgaaccg ctagcgcgga gccgcgacgt gctcgccggc ctgaagttga agggatgccg
     9361 cacccagcac gggaccataa tcgtgcgcct gcaaaatcac tgcgatgccg tcatgttttg
     9421 cattcgcgag attgagcggc aaatcgatct tcatcgcctc gccattccat tcgccggcgc
     9481 gctgcagatc gcgcaccaca ttggtgtagg tcaacgaatg attgcggttc tcgccctgct
     9541 cgacattgac cgtaccctga ttgagggtcc gcatcaccca gatcgtcgcg agcggtttgg
     9601 tctttgtctt cgccgccgga atggcgatct ccagccggcc tgacacggcg ccgacggtga
     9661 tcggaatcga ggtttccccg agcccgtcat tcgtgcgcgc cgccagtgcc gccattacgc
     9721 ggtcgcgttg attgccgacc acatcgaaac gcccgtcgac gatcatctgc ggcgtgtaga
     9781 tgcccgaatg gctcatcgcg cgcgcatagg atttctggcg ctgggtgttt gcgtcggtgg
     9841 cgtaggtatc cttccagccg agcatgtccc aataggtaat gggaagcgaa agggcgatca
     9901 cgtccttgcg tttggcgagt tccgtgacca gccggtccgc cgccggacaa tcgctgcagc
     9961 cctgactggt gaaaagttcg accacgatgg gccgggtcgc cgcggcgacc ggcgtctggc
    10021 gtgacgcagc cgcaggctgg cccgcccacg cgctaggact gagcgcggcg gccaggattc
    10081 ccgaaagaag aaaaaaacgc gcgtgcatgg gcaaaaccat aggatgaacg cgcgatttgc
    10141 ccaagagggt gggaatcacg ttgggttgac tgtccagccc ggatcacggt ctttttatgc
    10201 cgccgcgaga tagcgcagga cgtgctgaag gatgccgccg cttttgaaat aggcgacctc
    10261 gtccaccgta tcgatgcgcg caaccagcgg catgtcgacc gtacgcccat cgggataggt
    10321 cacacgcgcc gtcactttct tgcgcgtccc cgatgcccct tcccgcaccc cgatgtcgat
    10381 ggcttccttg cccgtcagcg cataatcgtt gcgtgtcttg ccgccggcga attcgagggg
    10441 caggattccc a
//
LOCUS       DASYEG010000300         6726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3305270, whole genome shotgun sequence.
ACCESSION   DASYEG010000300 DASYEG010000000
VERSION     DASYEG010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6726
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3305270"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VGM72_12760"
     CDS             <1..591
                     /locus_tag="VGM72_12760"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY4266184.1"
                     /translation="TAASGNAAATSFFPAKPLGCYGDGGACFTNDTALATVLRSLRMH
                     GEGTDRYEHARIGMNSRLDSIQAVVLSEKLKIFPDEIAARERIARRYNALLGKSNRIV
                     IPHIVAGAQSVWAQYTIQVEDRAKLLADMKDEGIPANVYYPIPLGRQRAYAHYPSVPT
                     PVSDALSASVASLPMHPYLDEATQDRIAAAVLKSVG"
     gene            591..1649
                     /gene="queA"
                     /locus_tag="VGM72_12765"
     CDS             591..1649
                     /gene="queA"
                     /locus_tag="VGM72_12765"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEY4266185.1"
                     /translation="MRVEEFDFDLPEGRIALRPLTPRDAARLMVVRADGGIEHRAVRD
                     LPELLKPHDCLVVNDTRVIPARLSGRRIARDEMGQGAAIEVTLHKRVSPSAFRAFVKP
                     AKRLKAGDRIMLGTTLDGTIAARDGGEIEIVFDRTGAALDAAIAEQGEIPLPPYIAGK
                     RKPDAQDRSDYQTVYADRPGSVAAPTAGLHFTPELFARLGEKGVQRADVTLHVGAGTF
                     LPVSVEDTKDHRMHAEWAELSLATADALNRIRESGGRIVAVGTTALRTLETAADDRGH
                     IHPFCSDTALFITPGYRFKAADVLLTNFHLPRSTLFMLVSAFSGTQIMRHAYAQAIAA
                     DYRFYSYGDACLLFRTAP"
     gene            1646..2779
                     /gene="tgt"
                     /locus_tag="VGM72_12770"
     CDS             1646..2779
                     /gene="tgt"
                     /locus_tag="VGM72_12770"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012270584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEY4266186.1"
                     /translation="MSGLGFEVLGTDGAARSGTITMKRGTIRTPAFMPVGTAATVKAM
                     LPESVAQTGAEIVLGNTYHLMLRPGAERVARLGGLHRFMNWQRPILTDSGGFQVMSLS
                     NLRKITEEGVRFRSHVDGAEHFLSPERAMEIQNLLGSDIQMVLDECAPFPATQTQIEE
                     SLALSMRWAKRSKATFDGGGGHACFGIVQGGVYPHLRRRSAEELKAIGFDGYAVGGLA
                     VGEGQPAMFETLEATLPNLPDDRPRYLMGVGKPDDIVGGVLRGIDMFDCVLPTRSGRN
                     GQAFTWKGPLNLRNARHADDTGPLDPECSCPACTSYSRAYLHHVVKAGEIIASMLLTW
                     HNLTFFQDLMRALREAIAGKRAAAFAKAFLTEYRPAVDTDAQG"
     gene            2815..2889
                     /locus_tag="VGM72_12775"
     tRNA            2815..2889
                     /locus_tag="VGM72_12775"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2847..2849,aa:Lys,seq:ttt)
     gene            complement(3152..3907)
                     /locus_tag="VGM72_12780"
     CDS             complement(3152..3907)
                     /locus_tag="VGM72_12780"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY4266187.1"
                     /translation="MSTILTRRALVTAGLTTAASAAGITAAARYGLIPSDYGTLFGAG
                     ETLTYSTQRLLTSGHSLAREFKRSDISRAPIVNGPAPPDDAFQRSASDGFKDWRLQFE
                     GLIERPVSFTLEELRNMPSESHIVLHACEQGWSYIAEWTGVRLSYVLNFVKTRPEARY
                     VVFMPYPNPQQTTGIIRVQWDSIDMADALHPQTILAYGMNREALPADFGAPLRLRMSR
                     QLGWKNTKYLTRILVTDRMNLYRKGAGTWYGGI"
     gene            complement(3904..4674)
                     /locus_tag="VGM72_12785"
     CDS             complement(3904..4674)
                     /locus_tag="VGM72_12785"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY4266188.1"
                     /translation="MRTGLSSASLDASVPAIPISDVPVVGVGARWTTQSLRHLAIVRV
                     THWVFATGVLGLIASGSGILISHPRLYWGETGSVAMPSLVDLPLPMIIGPSVWNRPIH
                     FFFAWFLVLGVLAYVSASITTQHLLKDLLPAKEDLRWRPILNVIWDHLRWRRTASDET
                     WTYNVVQRLTYLIVLFVLFPGIVWTGLAMSFAITSVFPFLVTVLGGHQSARTLHFVFA
                     SLLLMFLIVHIAMLCLVGFRAHVGAMITGRIPKGGSTQ"
     gene            5000..>6726
                     /locus_tag="VGM72_12790"
     CDS             5000..>6726
                     /locus_tag="VGM72_12790"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEY4266189.1"
                     /translation="MSDFAPAHLRKDTLRDFTADHRLLIIAAMSLVVGTMGAGAAWVL
                     MKLIALFTNLAYFQRLSIAPVAFSEQHLAPWTIVVPVVGGLIIGLIARYGSEKIRGHG
                     IPEAIEAILIGQSRIEPKVAALKPLSSAIAIGTGGPFGAEGPIIMTGGALGSLFAQMF
                     HVSPAERKTLLVAGAAAGMTAVFGTPIAAILLAVELLLFEWKPRSFIPVAVAAVVAAA
                     WRPLILGDGPLFPYAGVPDYPWWGLALCAAVGIAAGLQSAAMTICLYGMEDLFRKLPL
                     HWMWWPAIGGLFVGIGGYFEPAVLGVGYDSIAGLLHGTLDMRAAASLLFGKAAIWLVA
                     LSSGTSGGVLAPLLIMGGALGSLEAHVMPFGDTGFWALLGMAAILGGTMRAPLTATFF
                     AVELTGNTHIMLPLIVTSVAAFTLTVLVMRRSILTERIARRGHHITREYTIDPFLQTR
                     VSQIMARPVHTLSATTPCAEVVSFFTTIEGVRRHKSYPVIGDEQRLIGMVSRSDVMRW
                     VVEGVDDKVPLGDAVASATPIVTGYPDELVGDIADRMAAADVGRVPILERDSHKVVGL
                     VARRDLLRVR"
BASE COUNT         1292 a         2032 c         2065 g         1337 t
ORIGIN      
        1 accgcggcga gcggaaacgc cgcggcgacg agctttttcc cggcgaaacc gctcggctgc
       61 tatggcgatg gcggcgcctg cttcaccaat gacacggcgc tggccacggt gctgcgctcg
      121 cttcgcatgc acggagaagg caccgaccgt tatgagcacg cgcgcatcgg catgaactcc
      181 cggctggact cgatccaggc cgtcgtgctg tccgagaagc tgaaaatatt tcccgatgag
      241 atagcggcgc gcgagcggat cgcgcggcgc tataatgccc ttctcggaaa gagcaaccgc
      301 atcgtgattc cgcatatcgt cgccggcgcg caatcggtgt gggcgcaata tacgatacag
      361 gtggaagacc gcgccaagct gctcgcggat atgaaagacg agggcattcc cgccaatgtc
      421 tattatccga ttcccttggg gcggcagcgc gcctacgcgc attatcccag tgtgccgaca
      481 cccgtcagcg acgcattgag cgcaagcgtc gcaagccttc ccatgcatcc ctatctcgat
      541 gaggcgactc aggatcgcat cgccgcggca gtgctgaaga gcgtggggta atgcgtgtcg
      601 aggaattcga cttcgatctt ccggagggtc gtatcgcgtt acgtcccctg accccgcgcg
      661 atgcggcgcg gcttatggtg gtgcgggcgg acggcgggat cgagcaccgc gccgtgcgcg
      721 accttccgga attgcttaag ccgcacgatt gtctcgtcgt caacgacacg cgcgtcatcc
      781 cggcgcggct cagcggccgg cgtatcgcgc gggacgagat ggggcagggc gcggccatcg
      841 aggtcacgct gcacaagcgg gtctcgccct cggcgtttcg cgcctttgtc aaaccggcca
      901 agcggttgaa agccggcgac cgcatcatgc ttggaaccac gcttgatggc acgattgccg
      961 cgcgcgacgg aggtgaaatc gagattgtct tcgaccgaac cggcgccgcg ctcgatgccg
     1021 ccattgccga gcagggcgaa ataccattgc cgccttacat cgcgggcaaa cgcaaacccg
     1081 atgcacagga tcgcagcgac tatcaaacgg tctatgcgga taggccaggc tccgttgccg
     1141 cgccgacggc cggtttgcac ttcacgcctg aattattcgc tcgccttggc gagaaagggg
     1201 ttcagcgtgc cgatgtcacg ctgcatgtcg gggcgggcac gttcctgccg gtgagcgtcg
     1261 aagacaccaa ggaccaccgc atgcatgctg aatgggcgga gctttcgctg gccacggccg
     1321 atgcgctcaa ccgcatccgg gaatccggtg ggcgtatcgt tgcggtgggc acgacggcgc
     1381 tgcgcacgct ggaaaccgcc gccgacgaca ggggacacat ccatccgttc tgttccgaca
     1441 cagcgctgtt catcacgccc ggttaccgct tcaaggccgc cgatgttctt ctgaccaatt
     1501 tccacctgcc gcgctcgacc ctgttcatgc tggtctcggc cttttcgggg acgcaaatca
     1561 tgagacacgc ctatgcgcaa gctattgcgg cggattatcg cttctattcc tatggcgatg
     1621 cgtgtctgtt gttccggacg gcgccatgag cggtcttggt ttcgaagtcc tcggcaccga
     1681 tggggcggcg cgctccggaa ccatcacgat gaaacgcggg accatacgca cgcccgcctt
     1741 catgccggtg gggacagcag caaccgtgaa ggccatgctg ccggaaagcg tcgcacagac
     1801 gggcgccgag atcgtccttg gcaacaccta tcatttgatg ctgaggccgg gcgcggaacg
     1861 cgttgcgcgg ctgggcggcc tgcacagatt catgaattgg cagcgtccga tcctcaccga
     1921 ttctggcggc ttccaagtga tgtcgctctc caatttgcgc aagatcacgg aagagggggt
     1981 gcgcttccgt tcgcatgtcg atggtgcgga gcacttcctt tcgcccgagc gcgcgatgga
     2041 aattcagaac cttctgggct cggacatcca gatggtgctg gacgaatgcg cgccttttcc
     2101 ggcaacgcaa acacagatcg aagaatcgct ggcgctttcg atgcgctggg cgaagcggtc
     2161 caaagcgacc ttcgatgggg gcggcggcca tgcctgtttc ggcatcgttc agggcggcgt
     2221 ctatccgcat cttcgccggc gttcggctga ggaattaaag gcaatcggct ttgacggcta
     2281 tgcagtaggc ggtctggcgg taggcgaggg gcagccggcg atgttcgaga cgctcgaggc
     2341 gacgctgccc aatctgcccg acgaccgccc gcgttatctg atgggcgtgg gcaagcccga
     2401 tgacatcgtg ggcggcgtct tgcgcggcat cgacatgttc gactgcgtgc tgcccacccg
     2461 ctcgggccgc aacggtcagg ccttcacctg gaaggggccg ctcaatctgc gcaatgcgcg
     2521 ccacgccgat gacacggggc cgctcgaccc tgagtgttcg tgccctgcct gcacatccta
     2581 ttcgcgcgcc tatctgcatc atgtcgtgaa agcaggcgag atcatcgcct cgatgctgct
     2641 gacctggcac aatctgacct tctttcagga tctcatgcgc gcgttgcgtg aagcgatcgc
     2701 cggcaagagg gccgcagcgt tcgccaaggc cttcctgacc gaatatcggc cagcggtgga
     2761 taccgatgcc caaggttgac cccatcctcc ggtcgcctta catgctggcg ttgcgagccc
     2821 gtagctcagg ggtagagcat ctgactttta atcagagggt cgttggttcg ataccaaccg
     2881 ggctcgccaa taaaatcaaa cgcttaagta gatttttctt ctagaaaatg gttccgaaaa
     2941 gctcgtgggg acacaactga ggacccaatc cctggggtag gtggtgtcgc agttactcca
     3001 ttttggcggt cattaacggc taacccgtaa atttggcctc gaacttgcgc cttattaggg
     3061 gcgccacggt ccaaccctcc agtcaatcct gaaatccggg ctggttacgg acgcgcatta
     3121 acgcaccctc agactggcgc aacacgacgt ctcaaatgcc cccgtaccag gtaccggcac
     3181 ctttccgata aagattcatg cggtcggtga cgaggatgcg tgtcaaatat ttggtgttct
     3241 tccagccgag ctggcggctc atgcgcaggc gaagaggagc gccgaagtcg gcgggcaagg
     3301 cttcgcgatt cattccgtag gcgagaatgg tttgcggatg gagcgcgtcc gccatgtcga
     3361 tactgtccca ctgaacgcgg attatccccg tcgtttgctg agggttcgga tatggcatga
     3421 acacaacgta gcgcgcttcg ggtctcgtct tgacgaaatt cagcacatac gaaagccgca
     3481 ccccggtcca ttcggcaatg taggaccagc cctgttcgca ggcgtgcagg acgatgtggc
     3541 tttcggaggg catattccta agctcttcga gcgtaaagga taccggtcgc tcgatcaggc
     3601 cctcgaactg aagacgccag tctttgaagc cgtccgatgc cgagcgctgg aacgcatcat
     3661 ctggcggcgc tggcccgttc acaatgggag ccctcgagat atcgctgcgc ttgaattcgc
     3721 gagcgagcga atggcccgag gtaagaaggc gctgggtgga ataggttagt gtctctccgg
     3781 ctccgaagag ggtaccgtag tcggacggta tcaaaccgta acgggctgcc gccgtgatgc
     3841 ctgcggcgct tgcggcggtt gtcagacctg cggtgaccag cgctcgccgc gtcagaattg
     3901 tgctcattgc gtgctccctc ctttagggat gcgtccggtt atcatcgctc ccacatgggc
     3961 gcgaaacccg acaaggcaga gcatggcgat gtggacgatg aggaacatca gcaacaggct
     4021 tgcgaagacg aagtgaagcg tgcgcgcgga ctgatgacct cccaacacgg tcaccagaaa
     4081 gggaaatacg gaggttatcg cgaacgacat cgcaagacct gtccagacaa tgccgggaaa
     4141 cagcacgaac aggacgatca gataggtcag acgctgcacc acattgtaag tccacgtttc
     4201 atcggaggcc gtgcgccgcc agcgcagatg atcccaaatc acgtttagga tgggacgcca
     4261 gcgaagatcc tctttcgccg gcagcaagtc cttgagtagg tgctgcgtcg ttatactggc
     4321 gctcacataa gcaagaacac cgagcaccag aaaccaggca aaaaagaaat gtatcgggcg
     4381 gttccagacc gaagggccaa taatcatggg gagcggcaaa tcaacaagcg acggcatagc
     4441 aactgagcca gtttcgcccc aatacagtct cggatgagat ataagaatgc cactgccgct
     4501 agcgatcagc cccaggacgc ctgtagcaaa aacccagtga gtgacgcgaa cgattgccaa
     4561 gtggcgcaac gattgcgtag tccaacgcgc gcctactccc acgaccggca catcgcttat
     4621 tggaattgcc ggaacgcttg catcgagtga tgccgaggaa agacccgtcc gcatacggat
     4681 tgcctccaaa gaacagcacc cgccgtgatt tcacggtcca aacacaaagg tggtccctca
     4741 cattatcaca gcaacggcct ctaagtcttt gcgctaacgc ctgacatccg cgctaacgat
     4801 gccggaacga aaagtcttcg cgcgatgggc ggaacatccc tcgagcactc gctgcccgcc
     4861 ataactgcgg ccgcacggcg ggatattggt agcgcgacag aagcgcgcaa cctcgttgta
     4921 ctatagggca ttggggtata aaccgcgcgc cagtgcttca ggagtgaagc gcgcagtccc
     4981 accggaattc atcgctttta tgtcagattt tgctccagcg catctgcgta aagacacgct
     5041 gcgcgatttc accgccgatc accgattgct gataattgcg gctatgtcgc tggtcgtcgg
     5101 cactatgggc gcgggagcgg catgggtgct gatgaagctc atcgcgctgt tcacgaacct
     5161 tgcctatttt cagcgcctgt ccatcgcccc ggtggctttc tctgaacaac atctggcgcc
     5221 gtggaccatt gtggttcctg tggtgggtgg cctgattatc gggctgatcg cgcgctacgg
     5281 ctccgagaag attcgcggtc acggcattcc tgaagccatt gaagccattc taatcggcca
     5341 aagccgcatc gagcccaagg ttgccgctct gaaaccgctg tcatccgcca tcgccatcgg
     5401 caccggcggg ccgttcggcg ccgaagggcc gatcatcatg acaggcggcg cgctgggctc
     5461 gctgttcgcg caaatgttcc atgtcagccc cgccgaacga aagaccctgc tggtggcggg
     5521 cgcggcggct ggtatgacag ccgtattcgg cacgcctatc gctgctattc tcctcgccgt
     5581 cgagcttctg ctgttcgaat ggaagccacg cagtttcatc ccggttgccg tagccgctgt
     5641 cgtagccgct gcgtggcgtc ctctgatact gggtgacggg ccgctctttc cctatgccgg
     5701 cgtccccgat tatccatggt ggggccttgc actgtgcgca gccgtgggca ttgcagccgg
     5761 actgcaatcc gcggcaatga cgatatgcct ctacggtatg gaggatttgt ttcgcaagct
     5821 tcccctccat tggatgtggt ggcccgccat tggcggtttg ttcgtcggca tcggcgggta
     5881 tttcgagcct gcggtgttgg gtgtcggata cgacagcatt gccgggttgt tgcacggcac
     5941 gctcgacatg cgcgccgcgg cttcgctgct gtttggaaag gcggccatct ggcttgtggc
     6001 gctgtcctcc ggtacatccg ggggcgtgtt ggcgccgctg ctgatcatgg gcggcgccct
     6061 gggctcgctt gaggcgcatg tcatgccatt tggcgatacc ggattctggg cgctgctcgg
     6121 catggctgcg atcctcggcg gcaccatgcg tgcgccgctg acggcgacct tcttcgcggt
     6181 cgagctaacc ggcaacacgc atatcatgct accgctgatc gtgacttccg tcgcagcctt
     6241 cacgctgacc gtgctggtga tgcgccgttc cattctcacc gaacgcattg cgcggcgcgg
     6301 ccatcacatt acgcgcgaat acaccatcga tccgttcctg caaacgcgtg tttcacagat
     6361 catggctcgg ccagtacaca cgctgtccgc cacgacgcct tgcgcggaag ttgtctcctt
     6421 cttcaccacc atcgaaggtg tgcgccggca caagagctac cccgtcatcg gcgacgagca
     6481 gcggctcatc ggcatggtgt cgcgttcgga cgtgatgcgc tgggtcgtgg aaggcgtgga
     6541 cgataaggtg cccctgggcg atgccgtggc cagcgccacg cccatagtta ccggctatcc
     6601 cgacgagctt gtgggagaca ttgccgaccg catggcggcg gccgatgtcg gccgcgtacc
     6661 cattttagag cgcgacagcc acaaggtggt tggcctcgtg gcgcggcgcg atctgttgcg
     6721 cgtacg
//
LOCUS       DASYEG010000301        10632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4897921, whole genome shotgun sequence.
ACCESSION   DASYEG010000301 DASYEG010000000
VERSION     DASYEG010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 10632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10632
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4897921"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VGM72_12795"
     CDS             <1..289
                     /locus_tag="VGM72_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266190.1"
                     /translation="GTIDGEIAASQIALDKSNATTKIVPGHGPLTNKVEYQAYHDMLV
                     DIRGRVAKLKAEGKTEDQAVAAKPLADIQARLNQNDMASERVVRQVYETLK"
     gene            328..1428
                     /locus_tag="VGM72_12800"
     CDS             328..1428
                     /locus_tag="VGM72_12800"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4266191.1"
                     /translation="MKRFLAAFAVGALAMAVPGLVPAENSVAVAAQSGDLKIYWIDVE
                     GGASTLFVAPTGESLLFDTGNPGNGDRDAKRIFATAQKAGLTKIDHVVISHWHGDHVG
                     GLEALSKMIPLGKFYDHGDGVEQADKARHDAYVSLAGNNRKMLKAGDTIALGAIPVRV
                     VVSEGPVIANSINGGTANPLCANAPQMKPAAPENIRMVGLAITYNNFKYASLADLDWS
                     RETEMACPVNKLGHINLYTINRHGGLDDSGNPALLGAIRPQVIVLNNGPHKGLGQTVK
                     DAKPINANYTPYETNSYVRLAKNPDIQGVWAEHLSLIDKDPAHNAQPDMIANLEEGAG
                     DQGSGISAVVGADGKFTITNARNGFSKTYTAN"
     gene            1481..1912
                     /locus_tag="VGM72_12805"
     CDS             1481..1912
                     /locus_tag="VGM72_12805"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY4266192.1"
                     /translation="MNKTILPAVIVATGMVTAAALSYAAQSNPPANPPGDGFQPKPYF
                     VMPLEKDPTRQVRLQSVVIPPHVGNQFHRHPGDQWWAVQEGEVVYTIKGQAPRVLKPG
                     DYVYVPRGTIHRNQNMTDRPVRSIELNITDKDKPQTEPVPN"
     gene            2036..3109
                     /locus_tag="VGM72_12810"
     CDS             2036..3109
                     /locus_tag="VGM72_12810"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEY4266193.1"
                     /translation="MTLILSNTDIDSVLTMRECIDVLEDAYWELAHGRGVSRTRSDSF
                     SETKRPDALYSLKSMDGIVPKLGVGAVRINSDIITWPKQGENERRVKVPSAPDARYVG
                     LVLLFSTETGEPLAIFPDGVMQRMRVGAANALGVKYLARPKARQVAILGSGWQAGAQL
                     MGVCAVRDVEIIRCYSPNAQHCDAFAQEWSARLGVRVMPVPRAEDAIAEADIVMCATN
                     TIDNVFFARWIKPGLHISSIKRPEVETDAIKHADLVVLHSNDASPAHLFTRGVTIPET
                     AENKGWMLASEIAFDKLSTLPQLIAGLAPGRTREDQVTCFLNNIGLGYQFAACGALVY
                     RKAREQGLGRDLPTDWFTEDVHP"
     gene            3396..4400
                     /gene="msrP"
                     /locus_tag="VGM72_12815"
     CDS             3396..4400
                     /gene="msrP"
                     /locus_tag="VGM72_12815"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HEY4266194.1"
                     /translation="MLIRNKPSWELPERIASPEALVMNRRNLLRTAGAIAAAGVLAGC
                     DKNTAQNPTLDAAKVAAAKAPDPSASLYPFKTNEAFKAGREITPEELVSSYNNYYEFG
                     VEKDDPIRNADRLPIRPWEISFEGMVEKPFKTDFDTLLKAMPLEERVYRHRCVETWSF
                     AAPWSGFPMKALIDYAKPMSGAKYVVMKSWVNTEVAPAQNQVWYPWPYTEGLTVAEAT
                     NDLAFLVTGAYGKPLPKQNGAPLRLATPWKYGFKSTKGIQRFIFTIERPKTYWMALGP
                     DEYGFWANVNPNVPHPRWSQSSEEILGTGMRVPTKLYNGYGEQVAALYANLEGERLFM
                     "
     gene            complement(4611..5555)
                     /gene="trxB"
                     /locus_tag="VGM72_12820"
     CDS             complement(4611..5555)
                     /gene="trxB"
                     /locus_tag="VGM72_12820"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEY4266195.1"
                     /translation="MSDSVHAKVLILGSGPAGCTAAIYAARAMLEPTMVAGLQPGGQL
                     TITTEVENYPGFKDAIQGPWLMEQMQAQAEHVGTRVEYDLISSADLARRPFILTGDTG
                     RRYSTDALIIATGAQAKWLGLPSEERFKGFGVSACATCDGFFFKGKKVVVVGGGNTAV
                     EEALFLTNFATEVTLIHRRDTLRADKTNQMRFEQNPKTRIVWDSMVEDVIGSGEPRTV
                     SAVRVRNLKTGASDTLAADGLFVAIGHVPATEIFRGQLDMDEEGYIRTAHDSTKTSIP
                     GVFAAGDVKDKIFRQAVTAAGMGCMAALEAERFLAAGH"
     gene            5711..6190
                     /locus_tag="VGM72_12825"
     CDS             5711..6190
                     /locus_tag="VGM72_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019977129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY4266196.1"
                     /translation="MTRHRLDSIDRQILAELQADGRMTNVELARRVQITAPPCLRRVR
                     ALEKAGYVKGYHAELDAASLGFEVTAFAFVGLSSQAEADLKAFEDQARAWPQVRTCHM
                     LSGEVDFILLCVARDLPEFQTFVTEKLTAAKNVASVKTALAIRASKFEPGIPVEPRG"
     gene            6303..7181
                     /locus_tag="VGM72_12830"
     CDS             6303..7181
                     /locus_tag="VGM72_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266197.1"
                     /translation="MAEARTLEVGPGKSFSLPSQAAQAARDGDVVEIAPGTYIDCAVW
                     TSNGLTIESTGPGVILSKKSCAGKGIFVITGNDVIVRGLTFQHAAVPSRNGAGIRALG
                     GNLTVEHSTFLENEMGILATGSPAGSLIVRDSLFARNGSCYPNCRKDDFMHALYVGRE
                     LALFRVERSEFTGQHDGHYVKSRAARSEILNNVIHDGAKATSSYLIDIPDGGTVIAIG
                     NRLEKGPGFENSVAIAIGEESQKNTTPEIRIENNSFSNRNNKSAIFLWNRTKTEPLLR
                     ANTFEGKIVPLFTGMK"
     gene            7403..9415
                     /locus_tag="VGM72_12835"
     CDS             7403..9415
                     /locus_tag="VGM72_12835"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY4266198.1"
                     /translation="MGGASSILMDVPERSSRRRLIVFSCSFILACAVSLGYVYSRPAE
                     YRAIARVHISPAGVVAESTDTKSPALQGDPKSFLTEVQVLTSRPLLQEVVERIRTAGK
                     LPDLGKDPVDAVQQMLHAMPVEGTQVVELSAESSRQALVSDLVNTVAAVYREQTADAY
                     KDQVASTGANVDDEAQTLHDQVSAKQRALDAFRDRYDIVSLDRKENDVLAKIDGLTRS
                     YADGNAQLAKAQGNLEAFQAALAAGKPISRPRDDAAIAALETELSMLREKMRGLERRY
                     NSAFLVLDPETKALPAQIAELEQQLKDRRAESGRAAIADAEDELSSAKVTVARLRKDI
                     DDNQKGAREFATRLSEFKGMQEDVDHLQVMERQVLDRSTKLQSSIRERAPRVELIEAA
                     TPSLEPWRPNYRQNALISVAGSLFFGLLATWFADFLAGPAPARAMMVQHSLALPSVAR
                     YAAPALRTLKASPSDAPELHSPASQVQLLAPEGLPRELEDSEIAALIANASDDLRLAV
                     LTLLTGLDAAEVAALRWDRIDFGKRSILIGGPSPRTLALEDPLAAALEQRQPGSEPSA
                     PVLHDERGNPLSVEDLDRLVLFGAYDAGLERPADVNSAVLRHSYLAFLLRQGIRAADI
                     SRIAGHIPQAEMVAHMQSASPRARLPLEQIDRMHPVLRALASRFNA"
     gene            complement(9446..10090)
                     /gene="maiA"
                     /locus_tag="VGM72_12840"
     CDS             complement(9446..10090)
                     /gene="maiA"
                     /locus_tag="VGM72_12840"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEY4266199.1"
                     /translation="MMMKLYNFHASSTSYRTRIVLNLKGLAYAYIPIRLDKAEHLSAA
                     YGAINPMRGIPTLETDEGDRLYQSSAIIEYLEEVYPEPALLPKDAVARAHVRAIAGIV
                     GADMHPVNNLRVRNFIRDTYKQDADGVAAWIRLWNKAGFEAIETMLRADADRRGFCYG
                     EAPTIADAYLVSHVFSAVRFKTDMTPYPEIQSVVQNCTALKPFADAHPSKQPDA"
     gene            10216..>10632
                     /locus_tag="VGM72_12845"
     CDS             10216..>10632
                     /locus_tag="VGM72_12845"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HEY4266200.1"
                     /translation="MRRLFAFSVAGALLSGAALAASAVQPKPVDVLNTYGDLAHAMYE
                     DSFITAKALQGAVNAFLVDPTPAKLRVARDAWKAARVPYLQTEAFRFGNTIVDEWEGE
                     VNSWPLDEGLIDYVAASYGKTSDENPLYTLNVIANRQ"
BASE COUNT         2114 a         3148 c         3280 g         2090 t
ORIGIN      
        1 cggcaccatc gacggtgaga tcgcggcttc gcaaatcgcc ttggacaagt ccaacgcgac
       61 gaccaagatc gtgccgggcc acgggccctt gaccaacaag gtcgagtacc aagcctatca
      121 cgacatgctg gtggatatcc gcggtcgtgt cgccaagttg aaagccgagg gcaagaccga
      181 ggaccaggcg gtggcggcca aaccgctcgc cgatattcag gcacggctca atcagaatga
      241 catggcgagc gaacgcgtcg tgcgtcaggt ctatgagacg ctgaaataat ttcggctgag
      301 aacagggccg gacaaaggga gaaaacgatg aagcgcttct tggcggcatt tgccgttggt
      361 gcattggcga tggcggttcc gggtctggtg ccggccgaaa actccgtggc ggtggcagcc
      421 caatcgggcg atctgaaaat ctactggatc gatgtggagg gcggcgcttc aacgttgttc
      481 gtggccccga cgggcgagtc gctgctgttc gatacgggca atcccggcaa tggcgaccgc
      541 gatgccaaac ggatttttgc caccgcccag aaagccggcc tcaccaaaat cgatcatgtc
      601 gtcatcagcc attggcatgg cgatcatgtc ggcggtttgg aagcgttgtc gaagatgatt
      661 ccgctcggca agttctacga tcatggcgac ggcgtcgaac aggccgacaa agcgaggcac
      721 gatgcctatg tcagtctcgc cgggaacaat cgtaagatgc tgaaggccgg cgacacgatt
      781 gccttgggtg ccatcccggt gcgcgtggtg gtttccgaag ggccggtgat cgccaactcg
      841 atcaatggtg gtaccgccaa tccgctctgc gccaatgctc cgcagatgaa accggcggcg
      901 cctgagaata tccgcatggt cggccttgcc atcacctaca acaatttcaa atatgccagc
      961 ctcgccgatc tcgattggtc gcgggagacg gagatggcgt gcccggtgaa caagcttggg
     1021 cacatcaatc tctacaccat caaccgtcat ggcgggctcg acgattccgg caatcccgct
     1081 ttgctggggg ccattcgtcc gcaggtcatc gtgctcaata acggcccgca caaaggtctc
     1141 ggccagaccg tcaaggacgc caagcccatc aatgccaatt acacgcccta tgaaacgaat
     1201 tcgtatgtgc ggttggcgaa gaatccggac atccagggcg tgtgggctga gcatctgtcg
     1261 ctgatcgaca aggaccccgc gcacaacgcc caacccgaca tgatcgccaa tctcgaagaa
     1321 ggtgcgggcg accaggggag tggcatcagc gccgtcgtcg gtgcggatgg caagttcacc
     1381 atcaccaacg ctcgcaacgg cttcagcaag acctacaccg ccaattagcg acgagcggcg
     1441 caagcgtgtt cgagcaatcc gcgaatccag ggagaaagcg atgaacaaaa cgattctgcc
     1501 ggccgtgatc gtggcgaccg gcatggtgac ggcggcggct ctttcctatg ccgcacagtc
     1561 caatcctccg gccaatccac cgggcgacgg ttttcagccc aagccctatt tcgtgatgcc
     1621 gctggaaaag gacccgacgc ggcaggtgcg cctgcaatcg gtcgtcattc ccccgcatgt
     1681 gggaaaccag ttccaccgtc atccgggcga ccaatggtgg gcagtgcagg aaggcgaagt
     1741 cgtctacacc atcaagggcc aagccccgcg cgtactgaag ccgggcgatt atgtttatgt
     1801 tccgcgcggt acgattcacc gcaatcagaa catgaccgat agaccggtgc gctccatcga
     1861 gctgaacatc accgacaagg acaagccgca gaccgagccg gtcccgaact agcatcgtga
     1921 cttacccctg gtgggcgcgc cttgtgccgg tcgatttcgg ccgcgcacaa tctgggcggc
     1981 tcgtgccaca atgggtcatg gccgcaaccc tgcatcgcgc tgcacgagat tcgtcatgac
     2041 cctgatcttg tccaataccg acatcgattc cgtactgacg atgagggaat gcatcgacgt
     2101 tctggaggac gcctattggg agcttgctca cgggcgtggc gtctcacgca cccgttcgga
     2161 ttctttttcg gaaacgaagc ggcctgacgc gctctacagc ctcaaatcca tggacggtat
     2221 cgtcccgaag ctcggcgtcg gcgcggtgcg catcaattcg gacatcatca cttggcccaa
     2281 gcagggcgag aacgagcgcc gggtgaaagt gccctccgcg cccgatgccc gctatgtcgg
     2341 gctggtgctg ctgttctcga ccgaaactgg cgagccgctg gcgatttttc cggacggggt
     2401 catgcagcgc atgcgagtag gcgcggccaa cgcgctgggg gtcaaatatc tcgcgcgccc
     2461 caaggcccgg caagtcgcga tcctgggctc gggctggcaa gccggcgcgc agctcatggg
     2521 tgtgtgtgcg gtgcgcgatg tggagatcat ccgatgctac agcccgaatg cgcaacattg
     2581 cgatgctttc gcgcaagaat ggagcgccag gctcggtgtg cgggtgatgc ctgtgccgcg
     2641 cgcagaagat gcgattgccg aggcggacat cgtgatgtgc gcgaccaaca ccatcgacaa
     2701 tgtgttcttc gcccgctgga tcaagccggg cttgcacatc agctccatca aacgtccgga
     2761 ggtggagacc gacgccatca aacacgccga tctggtcgtc ttgcacagca atgatgcgag
     2821 cccggcgcat ctgttcacca gaggtgtcac cataccggaa accgccgaaa acaagggctg
     2881 gatgctggcg agcgagatcg cgttcgacaa attgtcaacc ctgccgcagc tcatcgccgg
     2941 cctggcgccg ggccgcacgc gcgaggatca ggtgacctgt tttctcaaca acatcgggct
     3001 tggatatcaa tttgccgcct gcggtgcgtt ggtctaccga aaggcgcggg aacaggggtt
     3061 ggggcgcgac ctgccgaccg attggttcac tgaagacgtc cacccgtaag ccaggcctgg
     3121 atggcaccgc tgctgctgct tcgaacgcac acaacattac aagaaattca tcgaccgttt
     3181 gcttggagtg cgggcctcat ggcggctttc gcggccatgc tcttttggta cgttccgctg
     3241 gggcgtcgtt gtctggctgc tgggccgtgg ttcgccgggg cgtggacttg gtctgcgggg
     3301 acgcggtgac tatattcatg acagaacata aatgtgagtt gccgtaggtt tcggacacgc
     3361 ttaggccgga atggccgagg ggcatggaga acgcgatgct gatcaggaac aagccgagct
     3421 gggagcttcc cgagcgcata gcgtcgccgg aagccctggt catgaaccgg cgcaacttgc
     3481 tcaggaccgc gggcgcgatc gccgccgcgg gagtgctcgc cggctgcgac aagaacacgg
     3541 cccagaaccc aaccctcgat gcggcgaagg tcgcggccgc gaaagccccg gatccgagcg
     3601 cctcgcttta ccctttcaaa acgaatgagg ctttcaaggc cggccgcgaa atcacgccgg
     3661 aagaactcgt ctccagctac aacaattatt acgagttcgg cgtggaaaag gacgatccta
     3721 tccggaatgc ggatcggttg cctatccggc cttgggagat cagcttcgag ggcatggtgg
     3781 aaaagccgtt caagacggat ttcgacaccc ttctcaaagc catgccgctg gaagagcgcg
     3841 tctaccgcca ccgctgcgtg gagacctggt cgttcgccgc gccttggtcg ggtttcccga
     3901 tgaaagcgct gatcgattat gccaaaccga tgtcgggcgc caaatatgtg gtgatgaaat
     3961 cctgggtgaa taccgaggtc gcaccggcgc agaatcaggt gtggtatccg tggccgtata
     4021 cggaaggttt gaccgtcgcc gaagcgacca atgatttggc tttcctggtg accggcgcct
     4081 atggcaagcc gctgcccaag cagaatgggg cgcccttgcg tttggcgacg ccgtggaaat
     4141 acggcttcaa atcgaccaag gggattcaac gcttcatctt cacaattgaa cggcccaaga
     4201 cctattggat ggcgcttggt cccgatgaat atggcttctg ggccaacgtc aatccgaatg
     4261 tgccgcatcc gcgctggagc caatcctccg aggagatcct cggcaccggg atgcgcgtgc
     4321 cgacaaagct gtacaatggc tatggcgagc aggtcgccgc gctctacgcc aatctggaag
     4381 gggaacggct cttcatgtga gccggtttgc ctttgcgcag aaaaaacggc cgggacgatc
     4441 gtcccggccg aggtgttttg gagggtgcga acggcatcgc cgtcagcatg ggagggaacc
     4501 ctccagcggt ttcgttcggg ggaaacgaac ctgatatcga tatgggtgag cgcgcggcga
     4561 ttttccaggg ttgcgtttgc cgtttttcga atcggcgaaa acgacttcgc tcaatgtccc
     4621 gctgcaagaa agcgttccgc ctcgagagcg gccatgcagc ccatacccgc cgccgttacc
     4681 gcctgacgga aaattttgtc cttcacatcg cccgcggcga agacaccggg aatgcttgtc
     4741 ttggtcgaat cgtgcgcggt gcggatataa ccctcttcgt ccatgtcgag ttgtccgcgg
     4801 aagatctcgg tcgccgggac atgaccgatg gcgacgaaca acccatctgc ggcgagcgtg
     4861 tcgctcgcgc ccgttttgag gtttctcacg cgtacggcag agacagtcct gggctcgccg
     4921 ctgccgatga cgtcctcgac catggaatcc caaacgatgc gcgtcttggg attttgttcg
     4981 aagcgcatct ggttggtctt atccgcgcgc aaggtatcgc gccgatggat cagggtcact
     5041 tcggtggcga aattggtcag gaaaagcgct tcttccacgg cggtattgcc gccgcccacc
     5101 acgaccacct tcttgccctt gaaaaagaac ccgtcgcatg tggcgcaggc cgagacgccg
     5161 aagcccttga agcgttcttc ggacggcaga cccagccatt tggcttgcgc gccggtcgcg
     5221 ataataagcg cgtcggtgct gtagcgccgg ccggtatcgc cggtgagaat gaagggcctc
     5281 cgcgccaggt cggcgcttga gatcagatca tattcgacgc gggtgccgac atgttcggcc
     5341 tgggcctgca tctgttccat cagccaaggt ccctggatcg cgtccttgaa tccgggatag
     5401 ttctcgacct ccgtggtgat cgtgagctgt ccgcctggct gcaggcccgc caccatggtc
     5461 ggttcgagca ttgcgcgggc ggcataaatc gccgccgtgc agcccgcagg accgctgccc
     5521 aggataagga ctttggcgtg tacgctatcg gacatggagt tccctggcag tcgactggcc
     5581 gtcacatatc ggaagaaggc actgaaaggt caagcaggcg attttttgct cacatctgcg
     5641 cgacaatgaa atattattgc tgaaacatga tcgcgtttgg cctagacggt gagcgtttta
     5701 aggacccagc atgacacgcc atcggcttga ttctatcgac cgccaaatcc tggccgaact
     5761 ccaggccgat gggcgcatga ccaatgtgga gcttgcgcgc cgcgtacaga tcaccgcgcc
     5821 cccatgcttg cggcgcgttc gcgcgcttga gaaagccggc tatgtgaagg gctatcacgc
     5881 ggagctggat gccgcctcgc tcggtttcga ggtcacggcc tttgcgtttg tcggactatc
     5941 gagccaagcc gaagccgatc tgaaagcgtt cgaggatcag gcgcgcgcct ggccgcaggt
     6001 gcgcacttgc cacatgcttt ccggtgaggt ggatttcatc ttgctgtgtg ttgcgcgtga
     6061 cctgcccgaa ttccagacct tcgttacgga aaagctgacg gcggcaaaga acgtcgcgag
     6121 cgtgaagacg gcattggcaa tccgtgcatc caaattcgag ccgggaatcc ctgtcgagcc
     6181 gcgtggataa accggtccgc cgtgctacca atcgcggcga aatcgtctgc ggcgtatcgg
     6241 tggcggctgt gcggtgtgct ccattccttg cgctatggtt tgcatgtgcg tgggcttcgg
     6301 cgatggcaga ggcgcggacg ctcgaggtcg gtccggggaa atcgttcagt ctgccgagcc
     6361 aggcggcaca ggcggcgcgc gacggagacg tggtggaaat cgcgcccggg acctacatcg
     6421 attgcgcggt gtggaccagc aacggtttga ccatcgagtc aaccgggcct ggtgtcatcc
     6481 tgtcgaagaa atcctgcgcc ggaaaaggca tattcgtcat cacggggaat gacgtcatcg
     6541 tgcgcgggct gaccttccag cacgcagcgg ttccttcgcg caacggcgca ggcatccgcg
     6601 cgctaggcgg caatcttacg gtcgagcaca gtacgttcct tgaaaacgaa atggggatcc
     6661 tggcgacggg ctctccggcg ggaagcctca tcgttcggga cagcctgttt gcgcgaaacg
     6721 gcagttgtta tccgaactgc cgtaaggacg atttcatgca cgcgctctat gtcggccgcg
     6781 agcttgcgct gtttcgggtg gaacgcagcg agttcaccgg gcagcatgac ggccattacg
     6841 tcaaatcgcg cgccgcgcgc agcgaaatcc tgaacaatgt gattcacgat ggtgccaagg
     6901 ccacgagttc ctatctgatc gatattcccg atggtggaac cgtaatcgcg atcggaaacc
     6961 ggctggaaaa ggggccgggc tttgaaaaca gcgtagccat cgcgatcgga gaagagagcc
     7021 agaaaaacac gacgcccgag atccggatcg aaaacaacag tttcagcaat cgcaacaata
     7081 aatcggcgat tttcctctgg aaccggacca aaaccgaacc attgctgcgc gcgaacacat
     7141 tcgagggcaa gattgtcccg ctgttcaccg gcatgaaatg acatccagga acaattatgt
     7201 gcgcaaaaca ggcgccggcg tttcgcgcga tgtgaaccta ttgcggcggg ccggcgcaat
     7261 ttaacgctgt ttgcggaacc cgattccgtt tcacgaaatt tcatgtacgc cttgccggaa
     7321 cggactgccg ggtctatttt cgtttcgcca gcctaattcc atgccgtgac ggattgccgg
     7381 gcttcgatgg agtcgttgtg tgatgggtgg cgcgtcctct atcctgatgg atgttccgga
     7441 acgttcttcc cggcggcggc tcatcgtatt cagctgctcg ttcattctcg cctgcgcggt
     7501 gagcctgggt tatgtctatt cacggcccgc ggagtatcgc gccatcgcgc gggtgcatat
     7561 ttctcccgcg ggcgtggtcg cggaatccac cgacacgaaa tcgcccgcgt tgcagggcga
     7621 tcccaaatcg ttcctgaccg aagtgcaggt tctgacgagc cgtcccttgt tgcaggaggt
     7681 ggtcgagcgc atcaggaccg cgggcaaact tccggacctc ggaaaggatc cggtcgatgc
     7741 ggttcagcag atgctgcacg caatgccggt cgaaggcacg caggtcgtgg agctttccgc
     7801 tgagagctct cggcaggcgc ttgtttccga tctcgtgaac accgtcgctg cggtctaccg
     7861 cgagcaaacc gccgatgctt acaaggacca agtggcgagc acgggagcca atgtcgatga
     7921 cgaggcccag accctccacg atcaggtgtc ggcaaaacaa cgtgcgctgg atgcgtttcg
     7981 cgatcgctat gacatcgtct cgctcgatcg caaggagaac gatgtcctcg cgaagatcga
     8041 tggcttgacc cgttcctatg cggacggaaa cgctcaactg gccaaggcac agggaaatct
     8101 ggaagcgttt caggccgcgc tggccgcagg aaagcccata tcccggccga gagacgatgc
     8161 ggccattgcc gcgcttgaaa cagagctttc catgcttcgc gaaaaaatgc gcgggctgga
     8221 gcgccgctac aattccgcct ttctggttct ggatcccgag acgaaggcgc ttcccgcgca
     8281 gatcgccgaa ctcgaacagc aattgaagga caggcgtgcc gagagcgggc gggcggccat
     8341 tgccgacgcg gaagatgaat tgtcgagcgc caaggtgacg gtggcccggc ttcgcaagga
     8401 tatcgacgac aatcagaaag gcgcgcgcga attcgccaca cggctttccg aattcaaggg
     8461 gatgcaggag gacgtcgatc atctgcaggt catggaacgc caggtgctcg accgctcgac
     8521 gaaactgcaa tcgagcatcc gcgagcgcgc gccgcgggtc gaactcatcg aggccgcgac
     8581 cccgagcctt gaaccatggc gtcccaacta tcgtcaaaac gcgctcatca gtgtggcggg
     8641 atcgcttttc tttggtctcc tggccacgtg gttcgccgat ttccttgccg gtccggcgcc
     8701 cgcccgcgcc atgatggtcc agcattccct ggcattgcca tcggtggcgc gctacgcagc
     8761 gccggctttg cggacgctca aagcctcgcc gtccgacgcg cctgagctcc attcgccggc
     8821 aagtcaggtc cagcttcttg cgcccgaggg tttgccgcgc gagctcgagg attccgagat
     8881 cgcagcattg atcgcgaatg cctcggacga tctgcgcctc gcggtcttga ccctgctgac
     8941 aggattggat gcagccgagg tcgcagcttt gcgctgggat cgaatcgatt ttggaaagag
     9001 atcgatcctt atcggcggtc catcgccgcg gacactcgcg ttggaagatc cgcttgccgc
     9061 cgcgctggaa cagcgccagc ccggttccga gccgtctgca ccggtcctgc acgacgaacg
     9121 cggcaatccc ttgagcgtcg aagacctcga ccggctcgtc ctgtttggcg cctacgatgc
     9181 cggcctggaa cggccggcgg acgtcaattc ggccgtgctg cgccatagct atctggcatt
     9241 cctgctgcgc cagggtatcc gcgcggcgga catcagccgc atcgcgggcc acattccgca
     9301 ggcggaaatg gttgcccaca tgcaatcggc atcgcctcgc gcacggttgc ctttggagca
     9361 gatcgaccgc atgcatccgg tattgcgcgc gctcgccagc cgcttcaacg cctgaaattt
     9421 ctgtttcgcg cgcgcgttcg cgcgcttacg cgtccggctg tttggacgga tgggcgtcgg
     9481 caaacggttt tagcgcggtg caattctgaa cgaccgactg gatttccggg tacggggtca
     9541 tgtccgtctt gaatcgcaca gcggaaaaaa cgtgcgacac cagataggcg tccgcgatcg
     9601 tcggcgcttc gccataacag aaaccccgcc ggtccgcgtc cgcgcgcagc atggtttcga
     9661 tcgcctcgaa gccggctttg ttccagagcc gaatccacgc ggcgacgcca tcggcatcct
     9721 gtttataggt gtcgcggatg aaattgcgga cgcgcagatt gttgaccggg tgcatgtccg
     9781 ctcccacgat tccggcgatg gcgcgcacat gcgcgcgtgc gaccgcatct ttcggcagca
     9841 gcgcaggctc gggatagacc tcctcgagat attcgatgat ggcggaggat tgatagagcc
     9901 ggtccccttc gtcggtttcc agggtcggga tcccgcgcat cggattgatc gcgccatagg
     9961 cggcgctcag atgctcggcc ttgtccagcc ggatggggat gtacgcatag gcaaggccct
    10021 tgaggttcag cacgatccgg gtgcggtagg acgtactgct ggcgtggaaa ttataaagct
    10081 tcatcatcat atctccctgt tcgtgccggg tttttcaccg ttcgccgatc ggatactaaa
    10141 tccattgtca ttttagctgc acttgcaaat cagtcgcata tgtggttccc atgggcgttc
    10201 agaacggacc tttccatgcg cagactcttc gcattttccg ttgccggtgc gctgctttcg
    10261 ggcgcggccc tcgctgcatc cgcggtgcag cccaaacccg tcgatgtgct caacacctat
    10321 ggcgacctcg cgcatgcgat gtacgaggat tcctttatca cggcaaaggc cctgcaaggg
    10381 gcggtgaacg cgttccttgt ggaccccacg cctgcgaagc tcagagttgc gcgtgacgcc
    10441 tggaaggcag cccgggtccc ttatcttcag accgaagctt tccgtttcgg caacaccatc
    10501 gtcgatgaat gggaaggtga ggtgaattcc tggccgctgg atgaggggtt gatcgattac
    10561 gtcgcggcgt cctatggcaa gacctccgac gaaaatcctc tgtacacgct gaacgtcatc
    10621 gccaataggc ag
//
LOCUS       DASYEG010000302        20815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2727065, whole genome shotgun sequence.
ACCESSION   DASYEG010000302 DASYEG010000000
VERSION     DASYEG010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 20815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20815
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2727065"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VGM72_12850"
     CDS             <1..238
                     /locus_tag="VGM72_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266201.1"
                     /translation="HTDKMHVIERWKIIDGGNTVEVSMNVEDPGAFTTPWSATQRWRR
                     VHQSPMMEIACNENNANYLEQYRFPMPTAEKSDF"
     gene            414..1328
                     /locus_tag="VGM72_12855"
     CDS             414..1328
                     /locus_tag="VGM72_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266202.1"
                     /translation="MSVFKFVPNPKARRLARLLCGGACAVVLSTASAPAQNNFLDDGN
                     VASLNTKVTIHDLAPDTVTSWVPDRPTGDDFLPPPSGPGPVMSPKDRPYVPNGNGRQP
                     TYRIADTSNPILKPWVVAQMEKSNADVRAGKVPYITRERCWPAGVPGFTVYTRVQPIH
                     FYETKKEITIINELNTQIRHIYMDVPHTKNPGHTWYGESVGHYQGDELVVDTIGLNDK
                     TYVDNYRTPHTDQIHVVERWKLIEGGKILQASVTVEDPGAFNMPWTGVQRWKRIARPL
                     EEYLCEPSNDGYFNYDVVPLPQANKSDF"
     gene            1358..2218
                     /locus_tag="VGM72_12860"
     CDS             1358..2218
                     /locus_tag="VGM72_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266203.1"
                     /translation="MGRTVLMRAGAAVIAITVLSGAASVPALGVESAAHPGAILNLGH
                     ADSGWFPVGDDFLPPISGPGPVISDPAHPYFNNQSGRQPTDRVADLTNPILTPWALER
                     MEKSNAATLAGKIPFSPRETCRPAGVPGFDVFSRLRPVYFLQTPKEVTIINEGDQQSR
                     RVYLNVPHSKNPKPSWYGESVGHYEGDTLVIDTIGMNGKTYVDNYLTPHTTALHVVER
                     FRLIEGGKMLEAMITVEDPEAFTMKWSARQRYRRQPQETMQEAVCAENNVDFFAQGYF
                     PVPQADKPDF"
     gene            2243..2491
                     /locus_tag="VGM72_12865"
     CDS             2243..2491
                     /locus_tag="VGM72_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266204.1"
                     /translation="MAMHPSTSFTWWNVFRMIDGGKTLEDFITVEYPGILTTPWPAAP
                     RFRRENNSPHVEDICAENNFAYFGYDAVPLPQAVRADF"
     gene            2530..3408
                     /locus_tag="VGM72_12870"
     CDS             2530..3408
                     /locus_tag="VGM72_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266205.1"
                     /translation="MSKFLKAATQRMSAGAMLAGLAAAGAFLLPASAADQTHAIPIFT
                     PAPNAGWVPDRITGDDFLPAPGGGPGPVMSDPKHPYVPNGGGRQSTYRVADLSNPILK
                     PWAIEKMKKANDDVLAGKVPYITRERCWPAGVPGSTVYTRGGQPLFFYQTAAEVTIVN
                     ELNAEVRHVYLGVPHSKNPKPSWYGESVGHYEGDELVVDTIGMNDRTTVDNYRTPHTG
                     KIHVVERFRLIEGGKTLQSTVTVDDPDTFNMPWTGVQRWKKTDRILEENPCAENNTEF
                     FGYDVVPVPHADKADF"
     gene            3646..4527
                     /locus_tag="VGM72_12875"
     CDS             3646..4527
                     /locus_tag="VGM72_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266206.1"
                     /translation="MPQTGSRRPRVSRKQRLWASAAVPLLAAASLWAIGASAADISIP
                     DFSQGNTAGWISGGADLLPPPSGPGPVGFDPAHPHVGNNDRQGRQPTPRIGDANSPIL
                     QPWAAQMVKKANEDVLAGKVGFEAMSTCWPAGVPGIMVFTAEPIYFVQTPKRVTILYQ
                     RGPNQRRVYMNVPHSKNPKPSWYGESVGHYEGKTLVIDTIGMNDKTFVDNVRTPHTTR
                     LHVVERYTMSDDGRSMDAMATVEDPGAFTTQWSAVQHYRRVTQPYSESVCAENNNNYN
                     YFNLNVDPIPEAAKPDF"
     gene            4549..5442
                     /locus_tag="VGM72_12880"
     CDS             4549..5442
                     /locus_tag="VGM72_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266207.1"
                     /translation="MRKPMFELATRKACAGFLFAIAIAGPIVIPAALAAPGTSQKIPA
                     LASPDYGWQTNVADWQEPPASYGHGPIRPDPQHPFISNAEAGRRGIQPTLRIGDVKDP
                     ILKPWAAQFMAASNKEALTGERQVPFAAQARCYPGGVPGQLLFPFEPVYFVQTPKEVW
                     MMWQRDHMVRRVLLTDRHSAKLKPSWFGESIGHYENGDTLVVDTVGLSTKNSYIDNFR
                     TPHSEKLHVIERFTVSADGKNLTAIATVEDPETFNGPLTMQQRWFKVDAPMAETSCAE
                     NNDDFFHQNLFPLPEAKTPDF"
     gene            5466..6434
                     /locus_tag="VGM72_12885"
     CDS             5466..6434
                     /locus_tag="VGM72_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266208.1"
                     /translation="MPRVASNGCVSAIFKHALAGAAFTGLLGMALTPAASAPAPGREP
                     ALVDRSTPSNLDFAVSKNGIPNLASISFAWLSDGADWIDPPAGTPGHGRIKNDPDHPF
                     LGNVDARRLGRQPTLRIGDYRDPVLKPWASKFMQETNAEALSGKLDVPFAAQARCWPG
                     GVPGQLLYPAEPFYFIQTPKEVWMIWQRDHMVRRIYLTDKHSANVKPQSFGESIGHYE
                     GGNTLVVDTVGLSTKNNYIDNFRTPHTTKEHVVERFTIAPDGKKLSAIVTVDDPDTFN
                     EPIHMMQTWHKVVNPLLETVCAENNNDSTFFHQKLFPMPEATKADF"
     gene            6474..7328
                     /locus_tag="VGM72_12890"
     CDS             6474..7328
                     /locus_tag="VGM72_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266209.1"
                     /translation="MSEFAFTMSAKLLFRGAAVIGVLAGLGGASSLAAGGAAPPDFGG
                     LSSGWRSAGGEWTMMPGSGQITVQDPQVRYVPNNVREQPTFRYAYARNPDLMPWANDA
                     LKKDNLKQDKGFAMWSRSSRCWAQGVPVFLLNPARPTYFIQTPKEVLMISETDHQVRH
                     IYMNVSHSPDLKPSWYGESVGHYEDSELVIDTIGQNTRTFIDNFRTPHGPRLHVVERF
                     RIIEGGKTMEEEITVEDAHTLLRPWHVTHRARRVNAPMQESSCAESGGDHFAVDAEPI
                     PTADKPDF"
     gene            7341..8165
                     /locus_tag="VGM72_12895"
     CDS             7341..8165
                     /locus_tag="VGM72_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266210.1"
                     /translation="MRTSLGMIGALLVGTTVSVAAPADHAAVPDFSGMTTGWLAMGTD
                     FKQVPGAPAMVTNDDARPHVGNGGGKQSSFRFADLDNPNLTEFAKAGLKKTNDEVLRG
                     NAVFSREARCWPTGVPTYDLNQAQPIYFIQTPKEVTIIWQMDHQVRHIYLNIAHSPNP
                     KPSWYGESVGHYEGDTLVVDTIGQNTKTYVDNYRTPHSEKLHVTERFHLTNEGKNLQA
                     DIMVDDPATFKQPLKLIQTWRKVAQGPMNESSCAESGPGFFDFDVEPIPTAGRPDF"
     gene            8165..9040
                     /locus_tag="VGM72_12900"
     CDS             8165..9040
                     /locus_tag="VGM72_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266211.1"
                     /translation="MALFSSTAKRRGAIAGGFAAAFFTVGSAFLGVSPVRAAAEKIPD
                     FSSDATVGWLSAGTEFAAMPSGPHPVTPDPAHPYILNGRRGRQPTLRVADLDNPILQP
                     WVKEALKKVNDRVLSGKAAFPPQVRCWPLGVPAFVLYPAQPVFFIQTPKEVLLIWQSD
                     QMVRHVFLTGKHSVNPKPSWFGESIGHYENGDTLVVDTIGLNTKTFVDNYRTPHTEQL
                     HVVERYKLMNAETRIEVTLTVEDPGAFTMPWSARQTYSRVEVGPMGESTCAEGNFNYF
                     GLDLDPLPEDDHPAF"
     gene            9062..9910
                     /locus_tag="VGM72_12905"
     CDS             9062..9910
                     /locus_tag="VGM72_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266212.1"
                     /translation="MAATMMRLKTTLAGCAFVLFLGSAALSAQEALNEQTPIPDFSSG
                     EASWIAQGVNFQLPSDGPHQITNDPAHPFKPPGRGEAPTFRVADLDNPILQDWTREEL
                     RKRNEHILGGGTGYTRQVSCWPMGVPAFLLYPAQPLYIVQAPKQVLLIAQMNNEPRHV
                     YLNVPHSRNVKPSWYGESIGHYEGGTLVVDTIGMNNQTWIDNYRTPHSDKLHVVERFH
                     IAEGGKTLQVDVHVEDPGAFTMPWDTVQRYDRTQQGALVEFPCAENNADFGNLDRMPI
                     AEKPDF"
     gene            9927..11588
                     /locus_tag="VGM72_12910"
     CDS             9927..11588
                     /locus_tag="VGM72_12910"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HEY4266213.1"
                     /translation="MSRYAFPRTLVSGVAAAALSALSAPAQAAMSCADLAGMKIAPAD
                     IGLPTSGAVVASAQVQSVPADPTGPGATREYCKILGAIAPVDPNAPPVNFEVNLPTQW
                     NGKAVQYGGGGTNGTLVTGLGPLRDARRDTPVPIARGFATWGTDAGHQNAKLPEPDAF
                     ALNQEALVNMAYASYKKTHDVGVRIATAFYGRTPAKIYYYGGSEGGREALMMAQRFPA
                     DFDAIVSVVPVANYVGINLVRDKIAQLQQDGGWISPAKVKTMQNAVNAACDKLDGLAD
                     GVIGAFERCRSVFDAKTLRCANGTDTGDTCLSDAQIEADRVLHQPYAYPFALANGVTS
                     FPGWTYGSEEQAGGMVASVTGLEPAHFPIVSAKVQSISWTGTNGFMRFLIARDASFNP
                     LHFSAQEFIPRIREVSEMFDTTNPDLSAFLARGGKLILKGNGADYRRSILQEITYYKS
                     VAAKMGQARVDGFIRFYVTPGVDHPGNGVMSSGAAVPAKVDLLGALDGWADSGKAPGE
                     LTQVSQETKAPFKIVASRPMCLYPAFPHYKGEGDPNDAASFTCAK"
     gene            11742..12608
                     /locus_tag="VGM72_12915"
     CDS             11742..12608
                     /locus_tag="VGM72_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266214.1"
                     /translation="MAGFSYGATSRRLLMGFAWFSLMSAGAALAADSGSQASSIPDFS
                     SFSNGWIAINSDFTPAAGSGPKPVTYDPKYPFRRNDEPGPATYRIADLNNPNLQPWAI
                     AELKKYNDRVLAGELGTTPRWSCLPGGVPGFSLFVVEPVYVVQSPKEVLLIYSGNREV
                     RHIYLNVPHSKAPKPTWYGESVGHYEGDTLVVDTIGLSTKAVIDNYHTPHTEQLHVVE
                     RYHLTEGGKQLEVNLLVEDPGTFKAPWQAIQHYSKVPNRPMLEQNCSENNDNIFDLPG
                     WIGPPTASKPDF"
     gene            12652..13482
                     /locus_tag="VGM72_12920"
     CDS             12652..13482
                     /locus_tag="VGM72_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266215.1"
                     /translation="MMKSIGAKLALIGVLLYAGGALAADGDAVPQFSFMDGGWQAAGA
                     DFLQPANGPGPVRDVPGHEHGGNNTGAQPTFRMADLSNPILQPWVRDALKKVNDKIAA
                     GGGGFTPQVSCIPLGVPAYLLHPAQPVYFIQTPKEVVLIWPPNHEVRRIYLIGKHSTN
                     VKPSWLGESIGHYEGGDTLVVDTIGLNDRTYVDNFRTPHTEKLHVVERFRINADAKGL
                     TVDVTVEDSGAFTMPWHAVQRYRRVQQGPMSESSCAENPTNFLGYDMDPIPTATKPDF
                     "
     gene            13517..14350
                     /locus_tag="VGM72_12925"
     CDS             13517..14350
                     /locus_tag="VGM72_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266216.1"
                     /translation="MFRFGPVRTAIPIFMLGIAALGTGGVLAQGVSDQPDFQSGGVSW
                     IAMNNDLAGPATGLGPTKPDPAHPYVANGQGTQPTFRIGDTGNPNLKAWAAERMKKDN
                     AEVLAGKIAYTARSSCMPAGVPGFSSYIVEPIYFIQTPKEILMVYAGNQEARHVYMNV
                     PHSLNPKPSWYGESVAHYEGDMLIVDTIGTNAKTFVDNYRTPHTEKLHVVERYRFADG
                     GKILNVEISVEDPDTFNQPWTAIQRYRKVQQGPLTEQSCAENNNGFFDYGIPTAAKAD
                     F"
     gene            14410..15297
                     /locus_tag="VGM72_12930"
     CDS             14410..15297
                     /locus_tag="VGM72_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266217.1"
                     /translation="MCGPNMWRRIAASLLAAAIATPVLALEKGAGFPLAPIGALKPRA
                     DGVPNLAAAGYGWFEDGDDFLPPVSGPGPVFSDPKHPYYSNQSGVQPTYRVADLSNPI
                     LRPWVKESLKKANDRALSGGVPFNPRERCWPAGVPAFEVYTRVRPVYFIQGRDKVLIV
                     NEGDAQVRHVYLNVPHTKNPRPSWYGESIGHYEGNNTLVVDTIGITDKTFVDNYLTPH
                     TGALHVVERFTLGRDGRTLSVMVTVEDPGAFTAKWSARQVYRLEFQGPWDESPCADNN
                     TNYFGFDVSPLPQARKPDF"
     gene            complement(15298..16704)
                     /locus_tag="VGM72_12935"
     CDS             complement(15298..16704)
                     /locus_tag="VGM72_12935"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4266218.1"
                     /translation="MSMAPVQAAEFARENCAGAGVSDSQTAAELIARLESVPFSRWHI
                     GPRVIMGSATFFDAFTALSLASATPVLVREWGLSPAQVGYLLAASYIGQFAGALIFGW
                     LGERIGRVISATYATLIMAVVSLACAFTGNFAQMFACRLIQGIGVGGEMPIAATYINE
                     LSRAHGRGRFFLLYELIFPIGFLAAAVLGARLVPLYGWNVLFLLGTVPGLIIAYLVSR
                     LPESPRWLIRKRRFAQAETIIQSLEASTQKRTPVAPHVMSAAAFAQRSRWGELFSPVY
                     RHRTLIVWVIWATAYGVTNGLNNWMPTLYNTVYHLPLQTSLNFALLTNTVQVFVLLVC
                     VFVIDRVGRKAWMAASFIIGGALLAPLGLFGAGDVNRVIIFVTLSYGVMSTVNTVLYL
                     YTPEIYPTRMRAIGTAAGTCWLRLASAAGPPIVGLTMVSHGIASVFLMFAGVAFLGAL
                     AATQMIETRNRRLEDIAP"
     gene            complement(16704..17516)
                     /locus_tag="VGM72_12940"
     CDS             complement(16704..17516)
                     /locus_tag="VGM72_12940"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4266219.1"
                     /translation="MTAQNSSFSTSDGCTISYTLQGAENANAPRLALVHSLALDRSVW
                     DGVAERLGGRAQILTYDARGHGRSSRPHMEYTAELFGRDLGELMDHVGWDAAVVAGCS
                     MGGCVAQAFAGLYPGRAAALALIDTTAWYGPEAPKQFRDRAAAAKAKGMQGLIDFQVT
                     RWFSEKFPPAHPERVKQATDVFLANDFECYAATCALLGDADLRHYLPAFKMPVAVVVG
                     EEDYATPIAMGQALHETIPQSTFRIIAGGRHLTPIECPGEIANEITKLLEHI"
     gene            complement(17585..18694)
                     /locus_tag="VGM72_12945"
     CDS             complement(17585..18694)
                     /locus_tag="VGM72_12945"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY4266220.1"
                     /translation="MPRLDPKTGLLYGNFMPLHPLWLTAALDHQRAGRLAEAECLYNE
                     VLQSDPRQGDALHLLGLIAASRGQFALAERRIADAISCQSRRGDFHASLGNLFFTRQM
                     IPQMTESYRRALLLTYFGAIPAPFPEIIVRAGSDADPTDFATEPAQYKSQYLQDVLLD
                     RWLFAGMTGGTFIDIGAHDGVTYSNSFFFETVRHWRGIAIEPNPDAFAKAAGSRKCTV
                     LNCCVGRESGTVRFLKISGYSEMLSGVLDNYHPEHRQRVEQEIEQFGGTAVVIPVETR
                     NLNEIAAQAGLSEITYLSIDTEGSELPILQSVDFGRLFVHAITVEYNFDPVKTRMISL
                     MDARGFDCAQTLGHDLLFLNRASPFHVRFMRLQKG"
     gene            18786..20009
                     /locus_tag="VGM72_12950"
     CDS             18786..20009
                     /locus_tag="VGM72_12950"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY4266221.1"
                     /translation="MRRRIFLQGLGGTFAAPAILSRALGAERPPLPSGLPQGEYDTVV
                     LEALPGKRPLIKHTARPPNYETPTALFATPVTPNDAFFVRYHLAGIPPRIDVRQWKLD
                     VGGEGVNTPLQLGFEDLQRGYEQIELTAVCQCSGNRRGLSDPHVPGVQWGLGAMGNAV
                     WRGPRLKDVLAKAGLKPGVVEIVADGADGPVLNSTPDFVKSLPLAKAMDDNTIIALQM
                     NGQDLPHFNGYPARLIVPGWTATYWVKHLTSLQAVNKPFEGFWMKAAYRIPTGMFPTV
                     QHFASQENAMNTPITEMVVNSLVVGPAEGARVRRGEALNVTGIAWDGGYGIRSVIVST
                     DSGMSWREAVLGADQGRFAFRPWSYRFTADAPGTHNILVRAINRLGETQVDTLIFNPA
                     GYHNNVVRPTTIMVS"
     gene            20022..20306
                     /locus_tag="VGM72_12955"
     CDS             20022..20306
                     /locus_tag="VGM72_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266222.1"
                     /translation="MKPLYVAVFAIMAVSVALAAESPVALKDSPGRATVENMCGGCHS
                     LDYIRINSPFMNRQTWTAEVNKMINAYGAPIPQNDTTAIIDYLTANYGEK"
     gene            20516..>20815
                     /locus_tag="VGM72_12960"
     CDS             20516..>20815
                     /locus_tag="VGM72_12960"
                     /inference="COORDINATES: protein motif:HMM:NF022409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 domain-containing protein"
                     /protein_id="HEY4266223.1"
                     /translation="MRIASNPPGQNIPEHNAPAQTRMETSMLSVNTNTGAMMALQFLN
                     QTQSDLQTTQSHINSGLKVASATDNGAVYAIAQNMRGSVSGYQAVTDSLNRGVSTV"
BASE COUNT         4432 a         6315 c         6079 g         3989 t
ORIGIN      
        1 gcacaccgac aagatgcatg tgatcgaacg ctggaagatc atcgacggtg gtaatacggt
       61 cgaggtttcg atgaatgtcg aggaccctgg cgccttcacg acgccctggt cggcgactca
      121 acgctggcga cgggtgcatc agagcccgat gatggaaatc gcctgcaacg agaacaacgc
      181 caattatctg gagcagtacc gctttcccat gcctacagcg gaaaaatcgg atttctaagg
      241 ctggtccgag ggcaaacgga gtggctcgcg cttctaaaaa caaaaccgct tgcaaaggac
      301 gcgcgcgcag gcggttttcg gttatagtaa tatgacattc cccggagagg gaaacccgtt
      361 cggcctgtgc ttcgctgaca aacacatcgc ctgacaaaca cattgtagga ggaatgtccg
      421 tgttcaaatt tgtgccgaat ccaaaagcgc gacgccttgc gcggctgctg tgcggcggtg
      481 cctgcgctgt ggttctctcc actgcaagcg cgccggccca gaacaatttt ctcgatgacg
      541 gcaatgtcgc atcgctcaat acgaaggtca cgatccatga ccttgcgccc gacacggtga
      601 cgtcctgggt gcccgaccgc ccgactggcg acgatttcct tccgccgcca agcggacccg
      661 ggccggtgat gtcgccaaag gaccgcccct acgtacccaa tggcaatggc cgtcagccga
      721 cctatcgcat cgcggacacc tccaatccga tcctgaagcc ctgggtcgtg gcgcaaatgg
      781 aaaagtccaa tgccgatgtg cgagcaggaa aggtgcccta catcacccgc gaacgttgct
      841 ggcccgccgg agtgccgggc ttcacggtct atacccgcgt tcagccgatc catttctatg
      901 agaccaagaa agagatcacg atcatcaacg agctaaacac gcagattcgc catatctaca
      961 tggatgtgcc tcacaccaaa aatcccggcc atacctggta cggagaatcc gtgggccatt
     1021 accagggcga cgaactcgtc gtcgatacga tcgggctcaa cgacaaaact tatgtcgaca
     1081 attaccgcac cccgcatacc gatcagatcc acgttgtcga acgctggaag cttatcgaag
     1141 gtggcaagat cttgcaggca agcgtcactg tggaggatcc gggcgcgttc aacatgccct
     1201 ggacgggtgt ccagcgctgg aagcgcattg cccgcccgct ggaggaatat ctctgcgagc
     1261 ccagcaatga cggctatttc aattatgacg tcgtgcccct tcctcaagcg aacaaatcgg
     1321 acttctgagg agagagccaa aggagaggaa acgacagatg ggcaggactg ttctgatgcg
     1381 cgcgggtgcc gcagtcatcg cgattacggt tctttccggt gctgcatcag tccctgccct
     1441 gggcgtcgag agcgcggcac atcccggcgc gatcctcaat ttgggacacg ccgacagcgg
     1501 ctggtttccc gtgggtgacg attttctgcc tcccatttcg ggtccggggc ccgtgatctc
     1561 cgatccggcg cacccctatt tcaacaatca gagcggccgt cagcctacag accgggttgc
     1621 cgatctcacc aatccgatcc tgacaccctg ggcgctcgag cgcatggaaa aatcgaatgc
     1681 cgcgacgctc gccggaaaaa tccctttcag cccgcgcgaa acctgccgcc cggccggcgt
     1741 tcccggcttc gatgtcttct cccgcctgcg gccggtctat tttctgcaga cgccaaagga
     1801 agtcacgatc atcaatgaag gcgatcagca atcgcgccgt gtctatttga acgtgccgca
     1861 ctcgaagaat cccaaaccgt cctggtacgg agaatcggtc gggcattacg agggtgacac
     1921 cctggtgatc gatacgatcg ggatgaacgg caaaacctat gtcgacaatt acctgacacc
     1981 gcacacgacg gcactccatg tcgtggaacg ctttcggctc atcgagggcg gcaaaatgct
     2041 ggaggccatg attacggttg aggaccccga ggccttcacc atgaaatggt cggcacgcca
     2101 gagatatcgt cgtcagccgc aggagacgat gcaggaagcg gtgtgcgcgg aaaataacgt
     2161 cgatttcttc gcacagggtt atttccccgt gccgcaagcg gacaaaccgg acttctgagc
     2221 cttttgcaaa ggagcattcc ccatggccat gcatccttca acaagcttca cgtggtggaa
     2281 cgttttccgg atgatcgacg gcggcaagac gctcgaggat ttcatcaccg tggaatatcc
     2341 ggggatcttg accacgccgt ggccggcggc cccacggttc cggcgcgaga acaatagtcc
     2401 ccatgtggag gatatttgcg ccgagaacaa ttttgcctat ttcggctacg atgccgtgcc
     2461 gctgccccag gcggtcagag cggatttctg aagaaactca gctattggca gagagtggag
     2521 gaagatcgca tgtcgaaatt tctaaaggcg gcaacgcaac gcatgagcgc gggcgcgatg
     2581 ctcgcaggtc ttgccgccgc gggcgcattt ttgctgccgg catcggccgc ggatcagacg
     2641 cacgcgattc cgatcttcac gccggccccg aatgcgggct gggtccccga ccgcattacc
     2701 ggagacgatt ttcttcccgc tcccggcggc ggcccgggcc ccgtgatgtc cgatccgaaa
     2761 catccctatg tgcccaatgg cggcggacgg caatcgacgt atcgcgtcgc cgacctgagc
     2821 aatccgatcc tcaagccgtg ggcaatcgaa aagatgaaaa aggccaatga cgacgtcctg
     2881 gccggcaagg ttccttacat cacgcgcgaa cgctgctggc cggcaggcgt tccgggttct
     2941 accgtgtata cgcgcggcgg acagccgctt ttcttctacc agacggcggc ggaggtcacg
     3001 atcgtcaatg agctgaacgc cgaagtgcgg cacgtctatc tcggcgtgcc gcattcgaag
     3061 aatccaaaac catcctggta cggagaatcg gtcggtcatt atgaaggcga cgaattggtg
     3121 gtcgatacga tcgggatgaa cgaccgcacc acggtcgata attatcgcac gccgcatacc
     3181 ggcaagatcc acgtcgtgga acgcttcagg ctgatcgaag gcggcaagac cttgcagtcc
     3241 accgtgaccg tcgatgaccc ggatacgttc aacatgccat ggaccggagt ccagcgctgg
     3301 aagaagacag accgcatcct cgaggaaaac ccctgcgcgg aaaacaacac agaatttttt
     3361 ggatacgatg tggttccggt accgcacgcg gacaaggcgg acttctaaga cgcggtccgg
     3421 cgggagttcc tagcctgccc gccttctcgg acatggcttt cacaaagcct tgaaaccggt
     3481 tctgccgcaa ttgtgcttgc gttgattttc gcccgaaccg gcccggaatg ccctgcccgc
     3541 ccgttgcccc gggggcttac gggccttaag ctcgcaattt gaacggcagc agagcgacga
     3601 ccctgcgcca acaaggtgcc tggagtggca agagaggagt tggaaatgcc gcaaaccggc
     3661 tcgaggcgcc cgcgcgtttc gcgaaagcaa aggctttggg cgagtgccgc cgttccgctc
     3721 ttggcggccg cttcgctttg ggctatcggc gcttcggctg cggatatttc cattcccgat
     3781 ttctcccagg gaaacaccgc cggttggatt tcagggggag ccgatttact gcccccacct
     3841 tcggggccgg gtccggtcgg gttcgatcct gcccatcccc atgtcggcaa caatgatcgc
     3901 caggggcgtc agccgacgcc ccgcatcggc gacgcgaata gcccgatcct gcagccctgg
     3961 gcggcccaga tggtgaaaaa ggcaaatgag gacgtccttg ccgggaaggt cggcttcgag
     4021 gcgatgtcca cctgctggcc ggccggcgtt ccggggatca tggtcttcac cgccgaaccc
     4081 atttatttcg tccaaacgcc caaacgcgtc acgatccttt atcaacgcgg acccaatcag
     4141 cggcgggtct acatgaacgt gccgcattcg aaaaatccga aaccctcctg gtacggagaa
     4201 tctgtcggcc actacgaagg caagaccttg gtcatcgaca caatcggcat gaatgacaaa
     4261 accttcgtgg acaatgtacg cacgccgcac accacgcgat tgcacgtggt cgaacgctac
     4321 acgatgtccg acgacggccg ctcgatggac gcaatggcca cggtggagga tcccggcgcc
     4381 ttcaccacgc aatggtctgc ggtgcagcac taccgccggg tgacacagcc ttacagcgag
     4441 tctgtctgcg ccgagaacaa caacaattac aattacttca atctgaacgt cgatccgatt
     4501 cctgaagccg ccaagccgga tttctgattt ttcgcgggta tgagacgcat gaggaaaccg
     4561 atgttcgagc ttgcgacgcg gaaagcttgc gcgggctttt tgtttgctat cgccattgcc
     4621 ggcccgattg tcattccggc tgcgctggcc gcccccggca caagccaaaa aattcccgcc
     4681 ctcgcctccc ccgattatgg ctggcagacc aatgtcgccg attggcagga gccgccggca
     4741 agctatggcc atggtccgat caggcccgat cctcaacatc ctttcatcag caatgcggaa
     4801 gccggacggc ggggcatcca gcccaccttg cgcatcggcg atgtgaaaga cccgatcctc
     4861 aaaccctggg cggcgcaatt catggccgcg agcaataagg aagcgctcac cggcgaacgg
     4921 caggttccct ttgccgcgca agcgcgctgc tatcccggcg gtgttccggg tcaattgctg
     4981 tttccgttcg agccggtcta tttcgtgcag acgccaaaag aggtttggat gatgtggcag
     5041 cgcgatcata tggtgcgccg cgtccttctc accgaccgtc attcggcgaa actgaagccg
     5101 tcctggttcg gcgaatccat cggccattac gagaacggcg atacgctggt ggtggatacc
     5161 gtcgggctct cgaccaaaaa cagctacatc gacaatttcc gcacacccca tagcgaaaag
     5221 ctgcacgtga tcgagcgctt caccgtcagt gcggacggta agaacctcac cgccatcgcc
     5281 acggtggaag accccgagac cttcaacggg ccactcacca tgcagcagcg ctggttcaag
     5341 gtggatgcgc caatggcgga gacgtcatgc gccgaaaaca acgacgattt ctttcatcag
     5401 aatctgtttc cgctgcccga ggcgaaaaca cctgatttct gatcggttct acacaggaga
     5461 atggaatgcc cagggttgcg tcgaacggtt gcgtttcagc gattttcaaa catgcgcttg
     5521 ccggcgccgc ctttaccggc ctactcggga tggcgttgac gccggcggcg tccgcaccgg
     5581 cgccgggacg tgagccggca ctggtggacc gcagtacgcc gtccaatctg gacttcgcag
     5641 tttccaagaa cgggattccc aatctcgcgt ccatcagctt cgcctggctg tcggacggcg
     5701 cggattggat agacccgccc gccggcacgc cgggtcatgg ccggatcaag aacgatcccg
     5761 accacccgtt cctgggcaat gtcgatgcgc gccggctcgg ccgtcagccg acgctgcgga
     5821 tcggcgatta tcgcgatccc gtgctgaagc cctgggcgtc caagttcatg caggagacca
     5881 atgcggaagc gctttcgggc aagctcgacg tcccctttgc cgcgcaggcg cgctgttggc
     5941 ccggcggtgt tccggggcaa ctgctttatc ccgcggagcc cttctacttc atccagaccc
     6001 ccaaggaagt ctggatgatc tggcagcgcg atcacatggt gcgtcgcata tacctgacgg
     6061 acaagcattc ggcgaatgtg aagcctcaat cctttggaga atccatcggt cattacgagg
     6121 gtggcaacac gcttgtcgtc gacaccgtcg gcctctcgac caagaacaac tacatcgaca
     6181 attttcgcac gccgcacacc acgaaagagc atgtggtcga acgcttcacg atcgcgcccg
     6241 acggcaagaa gctttcggcg atcgtcactg tcgatgatcc cgacacgttc aacgaaccca
     6301 tccacatgat gcagacctgg cacaaggtgg tgaacccgct gctcgagacc gtgtgtgcgg
     6361 agaacaacaa cgacagcaca ttctttcatc agaaattgtt cccgatgccg gaggcgacaa
     6421 aagcggattt ctgatcgccc gatggctcgg gacgttgggg ctgggaggaa ccgatgtccg
     6481 aattcgcgtt cacgatgtcc gccaagctcc tgtttcgagg cgcggccgtc ataggtgtgc
     6541 tcgcggggct tgggggtgcg tcgagtctcg cggcgggtgg tgcggctccg ccggatttcg
     6601 gcggtctgag ttcaggctgg aggtcggccg gcggtgaatg gaccatgatg ccgggaagcg
     6661 gccagatcac ggttcaggat ccgcaagttc gctatgtgcc caataatgtg cgcgagcaac
     6721 cgacgtttcg ctatgcctac gccagaaatc ccgatctcat gccgtgggcg aatgacgcac
     6781 tgaagaagga caatctgaaa caggacaagg gcttcgcgat gtggtcgcgc tcgtcgcgct
     6841 gctgggcgca gggcgtgcct gtctttttgc tgaaccccgc ccggccgacc tacttcatcc
     6901 agacgcccaa agaggtcctg atgatctcgg aaaccgatca tcaggtgcgg cacatttaca
     6961 tgaacgtatc tcattcgccg gatttgaaac cctcctggta cggagaatct gtcggccact
     7021 acgaggattc cgaactcgtg atcgatacca tcggtcagaa caccagaacc ttcatcgaca
     7081 atttccgcac gccgcacggc cccaggctgc atgtcgtgga acgcttccgg atcatcgaag
     7141 gcggcaagac catggaagag gaaatcaccg tcgaagacgc ccacacactg ctcaggccat
     7201 ggcacgtgac gcaccgcgcc aggcgagtga atgcgccgat gcaggaatct tcctgcgcgg
     7261 aaagcggcgg cgaccacttc gcggtcgatg ccgaaccgat ccccactgcg gataaacccg
     7321 atttctgatt ggaggttcat atgcgcacat ctcttggcat gatcggcgcc ttgctagtag
     7381 gaactacggt ctcggtggcc gcgcccgcgg accacgcggc agtccctgat ttttcgggca
     7441 tgaccacggg ctggctcgcg atgggcacgg atttcaagca ggtgccgggg gcgcccgcca
     7501 tggtcaccaa tgacgatgct cgtccccatg tcggcaatgg cggaggcaag cagtcgagct
     7561 tccggttcgc ggacctcgac aatcccaacc tcacggagtt cgccaaagcc ggcctgaaga
     7621 aaaccaatga cgaggtgctc cgcggcaacg ccgtgttctc ccgcgaggcg cgttgctggc
     7681 ccacgggcgt tccgacctac gacctcaatc aggcgcagcc tatctatttc atccagacgc
     7741 cgaaggaggt cacgatcatc tggcagatgg atcatcaggt gcgccacatc tatctcaata
     7801 tcgcccattc gcccaatcca aaaccctcct ggtacggaga atccgtcgga cattacgaag
     7861 gcgatacact ggtggtggac accatcggcc agaacacaaa aacttatgtc gacaattacc
     7921 gcacacccca tagcgagaag ctgcacgtga cggagcgctt ccacctgacc aatgagggca
     7981 agaacctgca ggccgatatc atggtcgacg atccggcgac gttcaaacaa ccgctgaagc
     8041 tgatccagac ctggcgcaag gtggcacagg ggccgatgaa cgaatcatcc tgcgccgaaa
     8101 gcggccccgg ctttttcgac ttcgacgtcg aacccatccc gacggccggc agacccgatt
     8161 tctgatggct ttattctctt ccacggcgaa gcggcgcggg gcgatagccg gtggctttgc
     8221 cgctgctttc ttcacagtag gctcggcatt tctgggcgta tctccagtcc gggccgcggc
     8281 ggaaaaaatt cccgatttct cttccgatgc gacggtaggc tggctatccg cggggacgga
     8341 attcgccgcg atgccgtccg ggcctcatcc ggtcactccc gatcccgcgc atccctatat
     8401 cctcaatggc cgccgcggcc ggcagcccac acttcgggtc gccgatctcg acaatcccat
     8461 ccttcagccc tgggtgaaag aggccctgaa gaaggtcaac gaccgcgttc tgtcgggcaa
     8521 ggccgcgttc cctccgcaag tgcgctgttg gccactcggc gtgcctgcct ttgtgctgta
     8581 tcccgcgcag cccgtgttct tcatccagac gcccaaggaa gtgctgctga tttggcaatc
     8641 cgatcagatg gtgcggcatg tttttctgac gggcaagcac tcggtcaatc cgaaaccttc
     8701 ctggttcggc gaatcgatcg gccattatga gaatggcgac acgctggttg tcgatacgat
     8761 cgggttgaat accaagacct ttgtcgacaa ttaccgcacg cctcataccg aacagctaca
     8821 tgtcgtggag cgctacaagc tcatgaacgc cgaaacgcgg atcgaggtta cgctcacggt
     8881 ggaggacccc ggcgcattca ccatgccttg gtcggccagg cagacctaca gccgtgtgga
     8941 ggtcggtccg atgggcgaat cgacatgtgc cgaaggcaat ttcaactatt tcggcctcga
     9001 tctcgatcct ctgcccgagg acgatcaccc ggctttctga caacccgtgc gagacaacgc
     9061 catggccgca acgatgatga gacttaaaac cactctcgca ggctgtgcgt ttgtgctttt
     9121 tctcggaagc gccgcacttt cggcccaaga ggcattgaac gagcagacac ccattccgga
     9181 tttttcctcg ggcgaggcca gttggatcgc gcagggcgtg aattttcagt tgccgtccga
     9241 cggaccgcac cagatcacca atgatcccgc gcatcctttc aagccgccgg ggcgtggcga
     9301 agccccgact ttccgggttg ccgaccttga caaccccatc cttcaagatt ggacgcggga
     9361 agagttgcga aagcgcaacg aacatatcct cgggggcggc acgggctata cccgtcaggt
     9421 gagctgctgg ccgatgggcg ttcctgcctt tctgctctac cctgcccagc cgctttatat
     9481 cgtccaggct cccaagcagg ttctcctgat cgcgcagatg aacaacgaac cgcgacacgt
     9541 ctatctcaat gtgccgcact ctagaaacgt gaagccatcc tggtacggag aatcgatagg
     9601 ccattacgaa ggcggcacat tggtggtgga caccatcggg atgaacaatc agacctggat
     9661 cgacaattac cgcacgcccc acagcgacaa gcttcacgtc gtcgaacgtt ttcacatcgc
     9721 cgaaggcggc aagacgcttc aggtcgatgt gcatgtggag gacccgggcg ccttcaccat
     9781 gccctgggat accgtccagc gatacgaccg gacccagcaa ggcgcactgg tggaatttcc
     9841 ctgcgccgaa aacaatgccg atttcggcaa tctcgaccgg atgccgattg cggaaaaacc
     9901 ggatttttga acgaggagaa aaaaagatgt cgcgatatgc ctttccccgt acgctggtca
     9961 gcggcgtagc cgctgccgcg ctttcagcat tatccgcccc cgcacaggcc gcgatgagct
    10021 gcgccgatct cgccggcatg aagatcgcgc cagccgacat cggtcttccc accagcggtg
    10081 cagtagtcgc gtccgcacag gttcagtccg ttcccgccga tcccacgggg cccggcgcga
    10141 cacgtgaata ttgcaaaata ttgggcgcga tcgcaccggt cgatcccaat gcgccaccgg
    10201 tcaatttcga ggtgaacctg ccgacgcaat ggaacggcaa ggccgttcaa tatggcggcg
    10261 gcggcaccaa cggcactctg gtcacgggtc ttggaccctt gcgcgatgcg agacgcgaca
    10321 caccggtccc gattgcgcgc ggctttgcca cctgggggac ggatgcaggc catcagaacg
    10381 cgaaacttcc ggaacccgat gcgtttgcgc tgaatcagga agccctcgtc aacatggcct
    10441 atgcctcata caaaaagacg cacgatgtgg gcgttcgcat cgcgaccgcg ttctacggcc
    10501 ggacacccgc gaaaatctat tattacggcg gttccgaagg cggtcgcgaa gcgctgatga
    10561 tggcacagcg cttcccggcg gatttcgatg ccattgtgag cgtcgtgccc gtggcaaact
    10621 atgtcggcat caatttggta cgcgacaaga tcgctcagct tcagcaggac ggcggctgga
    10681 tcagccccgc gaaagtcaaa acaatgcaaa acgccgtcaa cgccgcgtgc gacaagctcg
    10741 atggcttggc ggatggcgtt atcggcgcct tcgaaagatg ccgcagcgtg ttcgacgcga
    10801 agacgttgcg ctgtgcaaac ggcaccgaca cgggcgacac ctgcctatcg gatgcgcaga
    10861 tcgaggccga ccgggtcctg caccagccct atgcatatcc gtttgcgctg gctaatggcg
    10921 tgacctcgtt ccccggctgg acctatggca gcgaggaaca agcaggcgga atggtggctt
    10981 ccgtaacggg actcgagccg gcccatttcc cgattgtgtc ggcaaaggtg cagtcgatct
    11041 cgtggaccgg tacgaacggc tttatgcgat tcctgatcgc acgcgatgca tcgttcaatc
    11101 cgctccattt ctcggcccag gaattcatcc cgcgcatccg cgaggtctcg gaaatgttcg
    11161 atacgaccaa tcccgacctt tccgcatttc ttgcgcgcgg cggcaagttg atcctcaagg
    11221 ggaacggcgc cgactatcgc agaagcatct tgcaggagat tacctattac aaatccgttg
    11281 ccgcgaagat ggggcaggca cgggtggacg gattcatccg cttctatgtg accccgggcg
    11341 tcgatcatcc cggcaacggc gtcatgagtt cgggtgcggc cgtaccggcc aaggtcgatc
    11401 ttctgggggc gctggacggc tgggccgata gcggcaaggc tcccggagag ctgacgcagg
    11461 tgtcgcagga aaccaaggca ccgttcaaga tcgtggcgtc gcgcccgatg tgtctctatc
    11521 cggctttccc acattacaag ggcgagggcg atccgaacga cgcggcaagc ttcacttgcg
    11581 caaaatgacg atgagctacg ggtggcgaac agacgattga accgacgaaa tggaacggcg
    11641 gttgccgcgc atctttgcgc tggttgacgt caatccgaat tattcggtta tgatgtcaag
    11701 ccgataagaa ccgggaaata cgtttttcaa ggagcacgag tttggccgga ttttcgtatg
    11761 gagcgacgtc gcgtcgtctt ttgatgggtt tcgcctggtt ctcgctgatg tccgccggcg
    11821 cggcactcgc tgcggattcc ggcagccagg cttcgtccat tcccgatttt tcttcgttca
    11881 gcaacggctg gattgctatc aattcggatt tcactccggc agccggcagc gggccgaagc
    11941 cagtgaccta cgacccgaaa tatccgtttc ggcgcaacga cgagcccgga ccagcgacct
    12001 accgcatcgc cgatctcaac aatccgaatc tgcaaccctg ggccatcgcg gaattgaaga
    12061 aatacaacga cagggttctt gcgggtgaac tcgggacgac gccgcgatgg agctgcctgc
    12121 cgggcggcgt gccgggcttc tcgctgtttg tcgtggagcc ggtctatgtc gtgcaatcgc
    12181 cgaaggaagt gctgctgatc tattcgggca atcgcgaagt gaggcacatc tatctgaacg
    12241 tgccgcattc gaaggcgcca aagcccacat ggtacggaga atccgtcggc cattatgaag
    12301 gcgatacact ggtagtcgat acgatcggcc tctcgaccaa ggccgtcatc gataattacc
    12361 ataccccgca taccgagcag ctccatgtcg tggagcgcta tcatctgacc gagggcggca
    12421 agcagctcga ggtgaatctt ctggtcgaag acccgggcac cttcaaggcg ccgtggcagg
    12481 cgatacagca ttattcgaag gtgcccaacc ggccgatgct tgaacagaat tgctcggaga
    12541 acaacgacaa tattttcgat ttaccgggct ggatcggtcc cccgaccgcg agcaaacccg
    12601 acttctaagg caattgtgcg gttcatgcag ggggaagagg ggaagattgc cgtgatgaaa
    12661 tccataggcg caaaactcgc cctgataggc gtcctgctct acgcgggggg cgcgctggca
    12721 gccgatggtg acgcggtgcc gcaattttcc ttcatggacg gcggctggca ggcggcgggc
    12781 gcagatttcc ttcagcccgc gaacggcccc ggacccgtgc gtgacgtacc gggtcacgaa
    12841 catggcggca acaacacagg cgcccaaccc acattccgga tggcggatct gagcaatccg
    12901 atcctgcaac cctgggtgcg cgacgctctg aaaaaagtga acgacaagat tgccgcgggg
    12961 ggcggaggct tcacgccgca ggtgagctgc attccgctcg gcgttccggc ctatcttctc
    13021 catccggcac aacctgtcta tttcattcag acgccgaagg aagtggtgct gatctggccg
    13081 cccaatcacg aggttcgacg catctatctc atcggcaagc actccacgaa tgtgaaaccg
    13141 tcctggctgg gcgaatctat cggccattat gaaggcggcg acacgctcgt cgtcgatacg
    13201 atcggattga acgacaggac ctatgtcgac aatttccgca cgcctcatac ggaaaagctt
    13261 cacgtcgtgg agcgcttccg catcaatgcc gacgccaagg gcctgaccgt ggatgtcacg
    13321 gtcgaagatt ccggggcgtt cacgatgccc tggcatgccg tgcagcgtta ccgtcgcgtg
    13381 cagcaggggc cgatgagcga atcctcatgt gccgaaaatc ccaccaattt tctcggttac
    13441 gacatggatc ccattcccac cgcgaccaag cccgacttct gatttgcgaa ggcggcgttc
    13501 agtgaaggag gttgccatgt tccgtttcgg accggtccgg acagccattc caatattcat
    13561 gctgggaatt gccgcgctcg gcaccggggg cgtactcgcg cagggtgtgt ccgatcaacc
    13621 ggattttcag agcggcgggg tctcgtggat agcgatgaac aatgatctgg ccgggccggc
    13681 gaccggcctc ggccccacca aacccgatcc cgcgcatccc tatgttgcca atgggcaggg
    13741 cacgcagcca accttccgta tcggcgacac cggtaatccg aacttgaagg cctgggccgc
    13801 cgagcggatg aaaaaggaca atgccgaggt gctcgccggc aagatcgcgt acacggcgcg
    13861 ttcgagctgc atgcccgccg gagtgccggg attttcctcc tatattgtcg agccgatcta
    13921 tttcatccag acacctaagg aaatcctgat ggtctatgcg ggcaatcagg aagcgcggca
    13981 tgtctacatg aacgtgccgc actccctcaa tcccaagccc tcctggtacg gcgaatccgt
    14041 cgcccattat gaaggcgaca tgctgatcgt cgatacgatc gggaccaacg cgaagacctt
    14101 cgtcgacaat taccgcacgc cgcataccga aaagcttcac gttgtggaac gctaccgatt
    14161 tgccgacggc ggcaaaatct tgaatgtgga aatcagcgtc gaagatcccg acaccttcaa
    14221 ccaaccctgg actgcgatcc aacgctaccg caaagtgcaa caggggccgc tgacggaaca
    14281 gtcctgtgcc gagaacaata atggcttttt cgattatggg attccgacag ccgcgaaggc
    14341 cgatttctaa cggcacgcgt ggctgatcga gatcgtctca ctgaaatcag gccaaaatca
    14401 ggagtgagca tgtgcgggcc caatatgtgg cggcggattg cggcgagctt gcttgccgcg
    14461 gcaatcgcga cgccggttct ggcactggaa aagggtgccg gatttccctt ggcgcccatc
    14521 ggcgcgctca aaccgcgcgc cgacggcgtc cccaatctcg ccgccgcggg ctatggctgg
    14581 ttcgaagacg gcgatgattt tctgccgcct gtgtccggcc cgggaccggt attttccgat
    14641 ccgaagcatc cctattacag caatcagagc ggcgtccagc cgacctatcg cgttgccgat
    14701 ctctccaatc cgatcctgag accgtgggtg aaggagtcct tgaaaaaggc caacgatcgg
    14761 gcgctctccg gcggcgtgcc attcaatccg cgcgaacgtt gctggcctgc cggcgtcccg
    14821 gcattcgaag tttatacccg cgttcggccg gtctatttca tccagggccg agacaaggtt
    14881 ctgatcgtga acgagggcga cgcacaggta cgtcatgtct atctgaatgt tccgcacacg
    14941 aaaaatccac ggccatcctg gtatggggag tccatcggac attacgaagg caacaatacg
    15001 cttgtcgtgg acacgatcgg gatcaccgac aaaacctttg tcgacaatta tctgacgccc
    15061 cacaccggag cgctccacgt cgtggaacgc ttcacactcg gccgcgatgg caggacatta
    15121 agcgtgatgg taacggtcga ggacccgggc gccttcaccg cgaaatggtc ggcgcggcaa
    15181 gtttatcgtt tggaatttca aggtccatgg gatgaatcgc cctgtgcgga taacaacacc
    15241 aattatttcg gcttcgatgt ctcgcccctg ccccaggcgc gcaagccgga tttttgatca
    15301 gggtgcgatg tcttcgaggc ggcggtttct ggtctcgatc atctgcgttg cggccaaggc
    15361 acccaaaaac gccacaccag caaacatcag aaacaccgac gcgattccgt gggacaccat
    15421 cgtcaaaccc acgatcggcg gtcccgccgc ggacgcaagc cttagccagc aggttcccgc
    15481 ggccgtcccg atggcgcgca tgcgggtggg gtagatttcc ggcgtataga gataaagcac
    15541 ggtattgacc gtgctcatca cgccatagct caacgtcacg aaaataatca cgcgattgac
    15601 gtcgcctgcg ccaaacagcc cgaggggcgc cagaagcgcg ccgccgatga tgaaactcgc
    15661 tgccatccag gctttgcggc cgacgcgatc aatgacgaag acgcagacga gcaggacaaa
    15721 cacttgcacc gtattggtca gcagcgcgaa attcagcgag gtttgaagcg gcaaatgata
    15781 gaccgtgttg taaagggtcg gcatccaatt gtttaaaccg ttggtgacac cataagccgt
    15841 cgcccagatc acccacacga tcagcgtgcg atggcgataa accggtgaga acagctcgcc
    15901 ccaccggctc ctctgcgcaa aggcggcggc ggacatcacg tgcggcgcga ccggggttcg
    15961 cttttgcgtg ctcgcttcaa ggctttggat gatcgtttcg gcttgcgcga aacgtctttt
    16021 ccggatcagc cagcgcggag attccggcag acgcgatacg agataggcga tgatcaggcc
    16081 cggaacggtt ccgagcagga aaagcacgtt ccagccatag aggggaacaa gcctggcgcc
    16141 cagcacggca gcagcaagaa aaccgatcgg gaagatcagt tcgtacagaa ggaagaaacg
    16201 gccccgccca tgcgcccgcg acaattcgtt gatataggtc gccgcgatag gcatttctcc
    16261 gcccacgcca atgccctgaa tgagccggca tgcgaacatc tgcgcgaaat tgccggtgaa
    16321 ggcgcaggca agacttacga ccgccatgat cagggtggca tatgtcgcac ttatcacgcg
    16381 gccgattcgc tcgccgagcc agccgaagat caacgcaccc gcgaactgtc cgatatagga
    16441 ggccgcgaga agatagccca cttgcgccgg gctcagtccc cattcccgca ccaggaccgg
    16501 cgttgcggag gccaacgaca atgccgtgaa ggcgtcgaag aacgtggcac ttcccatgat
    16561 gacgcgcggg ccgatgtgcc atcgggaaaa cggcacggat tcgagtcgcg cgatcagttc
    16621 cgccgccgtt tgagaatcgc tgactcccgc accggcgcag ttttcccgcg cgaattcggc
    16681 tgcttgcaca ggagccatgg acatcagata tgctccagca attttgtgat ctcgttcgcg
    16741 atctcgccgg ggcattcgat gggtgtcagg tggcggcccc cggcaatgat ccggaacgtc
    16801 gattgcggaa tggtttcgtg cagcgcctgc cccatagcga tgggcgttgc ataatcttcc
    16861 tcgccgacga ccacggccac gggcatcttg aaagccggca aataatgtct gaggtccgca
    16921 tcgcccagga gggcacaggt tgccgcatag cattcaaaat cattggccag gaagacatcc
    16981 gtcgcctgtt tcacgcgctc aggatgggct ggcgggaatt tctcggaaaa ccagcgcgtg
    17041 acctggaaat cgatcaggcc ctgcatgcct ttggccttgg cggccgcggc ccggtcgcga
    17101 aattgtttgg gggcttcggg accgtaccag gcggtggtgt cgatgagggc gagtgctgcc
    17161 gcacgcccgg gataaaggcc cgcgaaagcc tgcgccacgc aaccgcccat ggaacagccc
    17221 gccacgacgg ccgcatccca gccgacatgg tccatcaact cgccgagatc gcgcccaaac
    17281 aactcggccg tatattccat gtgcggacgg ctggagcgcc catgcccgcg cgcgtcatag
    17341 gtgaggattt gagcacggcc gccgaggcgc tccgccacgc cgtcccagac gctgcggtcg
    17401 agcgcaagcg aatgaacgag cgccagccgc ggcgcgtttg cgttttcggc gccctggagc
    17461 gtgtaagaaa tcgtgcaacc gtccgatgtc gaaaaactgc tgttttgtgc tgtcatgtcc
    17521 caccaataat gaatgtggcg ggactgtaaa acagttcccg cgcgacacca atcgcctctg
    17581 atcctcagcc cttctgcaag cgcatgaacc gtacgtgaaa ggggctggcc cggttcagga
    17641 agagaagatc gtgcccgagc gtctgggcac agtcgaaacc gcgcgcatcc atgagagaaa
    17701 tcatgcgcgt tttcaccggg tcgaaattgt attcgaccgt gatggcgtgc acgaagagcc
    17761 ggccgaaatc gaccgattgc aggatgggca gttcgctgcc ttcggtatcg atgctgagat
    17821 aggtgatttc cgacagcccg gcttgggcgg cgatctcgtt caggttgcgt gtttcaaccg
    17881 gaatgactac cgccgtgccg ccgaattgtt cgatctcctg ttcgacgcgc tgacgatgct
    17941 cgggatggta attgtccagg accccggaca gcatctccga atagccggag atcttcagga
    18001 aacggaccgt tcccgattcg cgtccaacgc agcaattcag tacggtgcat ttgcggctcc
    18061 cggccgcctt cgcgaatgca tccggattgg gctcgatcgc gatccctcgc caatgacgca
    18121 ccgtctcgaa aaagaacgag ttgctgtaag tcacaccatc gtgtgcgccg atatcgatga
    18181 aggttccgcc cgtcataccg gcaaacaacc agcggtcgag aagaacatcc tgcagatatt
    18241 gggatttgta ctgcgccggt tcggtcgcaa aatcggtcgg gtccgcatcg cttccggcgc
    18301 ggacgatgat ctccgggaac ggcgccggaa ttgcgccgaa ataggtgagc agaagggcgc
    18361 gccgatagga ttcggtcatc tgcggtatca tctggcgcgt gaagaacaga tttccgaggc
    18421 tggcgtggaa gtcgccacgc ctggattggc aggagatcgc gtccgcgatt ctgcgctcgg
    18481 ccagcgcgaa ttgccccctg ctggccgcga tcagacccag aagatgcagc gcatcgccct
    18541 gtcgcgggtc ggattgaagg acctcattat agagacactc ggcctcggcg aggcggccgg
    18601 cgcgctgatg atccaatgca gcggtaagcc ataggggatg cagcggcatg aagtttccgt
    18661 acagcaggcc cgtcttcggg tccagcctgg gcatttatgt cagcgcatgc ggacagatgg
    18721 cgcttgcagg acatgcagtg ctatctcaca atcggcctca agtgttttta atacgcggag
    18781 gcaaaatgcg tcgcagaatt tttttgcaag gcctcggcgg cacattcgcc gcaccggcga
    18841 ttttgtcccg ggccctcggc gccgaacgtc cgccgctgcc ctccggcctg ccgcagggcg
    18901 aatacgacac ggtggttctc gaagcgcttc ccggcaaacg tccgctgatc aaacacaccg
    18961 cccggccacc gaattacgaa actccgacag cgctttttgc gacgccggtc acaccgaacg
    19021 atgcattctt cgtgcgttac catctggccg ggataccgcc gcgcatcgac gtgcggcaat
    19081 ggaagctcga tgtcggtgga gagggcgtga acacccccct gcagctcggc tttgaggatt
    19141 tgcagcgcgg ttatgagcaa atcgaactca ccgcggtttg tcagtgctcg ggcaatcgcc
    19201 gcgggctttc cgacccccat gtgccgggcg tgcagtgggg cctcggtgcg atgggcaacg
    19261 ccgtctggcg cggaccgcga ttgaaggatg tgctcgccaa agcgggattg aaaccgggcg
    19321 ttgtggagat cgtggccgac ggtgccgacg gtccggtcct caattcgacg cccgatttcg
    19381 tgaaaagcct gccgctcgca aaggcgatgg acgacaacac catcatcgcc ctgcaaatga
    19441 acgggcagga tcttcctcat ttcaacggct acccggcgcg cctcatcgtg ccgggttgga
    19501 ccgcgaccta ttgggtcaaa catctgacct cgcttcaggc ggtgaataaa ccctttgagg
    19561 gtttttggat gaaggctgcc tatcgcatcc cgacgggaat gtttccgacg gtgcagcact
    19621 tcgcgagtca ggaaaacgcg atgaacaccc ccatcaccga gatggtggtc aattcgctgg
    19681 tcgtgggccc tgcggaaggt gcgcgcgtgc gcagaggcga agccctcaac gtcaccggaa
    19741 tcgcctggga tggcggctat ggcatacgct cggtgatcgt gtcgaccgat agcgggatga
    19801 gctggcgtga agccgtgctc ggcgccgatc aggggcgctt cgcgttccgg ccatggagtt
    19861 atcgttttac ggccgatgcg cccggcacac acaacatcct tgtacgcgcc ataaaccggc
    19921 tcggagaaac gcaagtggat acgctgattt tcaaccctgc cggctatcac aacaatgtcg
    19981 ttcggccaac caccatcatg gtctcctgaa gggtgccttc aatgaaaccg ctctacgttg
    20041 ccgtctttgc catcatggcc gtgtcggttg cgcttgccgc agaatcgccg gtcgcgctca
    20101 aggattcgcc tggccgcgcc accgtcgaga atatgtgtgg cggctgtcac agtctcgatt
    20161 acatccgcat caattcacca ttcatgaatc gtcagacttg gacggccgag gtgaacaaga
    20221 tgatcaatgc ctatggtgcg ccgattccgc aaaatgacac caccgcgatc atcgattacc
    20281 tgaccgccaa ttacggcgag aagtaacggt taaggccaga gaagaaacag cgcaggatcg
    20341 gtcttcttgc gtgctgttgg ccggaactcg gcagaatttt ccggccactc ggcaaaaatt
    20401 tccctatgac ggcaagaatt gccgggcgaa cgcggcccga ttggtttccg cggggcagaa
    20461 attcccatgc cccctgcgtg caatgatcct taatcgatcc ctcaaattgc acggaatgcg
    20521 tattgccagc aacccaccgg gacaaaacat cccggagcac aacgctccag ctcaaaccag
    20581 aatggagaca tcaatgctta gcgtgaacac aaataccggc gcgatgatgg cgctgcagtt
    20641 cctcaatcag acccaatcgg atttgcagac cacccagagc cacatcaatt cgggcttgaa
    20701 ggttgcttcc gccaccgaca atggcgccgt ctatgccatc gcgcagaaca tgcgcgggag
    20761 cgtgtcgggc taccaggccg tcacggacag tctcaatcgc ggtgtttcca ccgtg
//
LOCUS       DASYEG010000303        23169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_406702, whole genome shotgun sequence.
ACCESSION   DASYEG010000303 DASYEG010000000
VERSION     DASYEG010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 23169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23169
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406702"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VGM72_12965"
     CDS             <1..583
                     /locus_tag="VGM72_12965"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY4266224.1"
                     /translation="WRSPGHGQAFPPYSVEPPHWPAVCLDWNDAKTYVVWLNGKAMAA
                     RPTLVDAPYRLPSEAEWEYAARAGTVTARWWGDAVGSGHANCNGCGSKFDYHVLADVD
                     SFPPNPFGLYGMLGNAWEWTEDCWHESYAGAPADGSAWTLGADCIKHVVRGGSWDNVP
                     VFVRAATRSGSAPNGGEFDYSSLAGFRVARTLP"
     gene            complement(594..1412)
                     /locus_tag="VGM72_12970"
     CDS             complement(594..1412)
                     /locus_tag="VGM72_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266225.1"
                     /translation="MLPSLLGEKDEAVPLRVNGVASYAKRPGLAISFVIHGLVAFFLI
                     FHMRAALPPSPPPAVPVDLVQLAEETIAPAPSRDRALPQRPQAATRQRQASLTPPRAP
                     QARTIERPAIPNPPAETAPPEQPRDELETKLQGLANLKQPSTDPRLLNGAGTAGINAN
                     GNGAPGPYAAYSVKDYIRAQVERRWNLNTEELGARNFTIPIHVVLAADGTVTKAEIVD
                     QKRYIADVAYRSIALSARNAVLLSSPFALPLGQVASQGGVMDMTLDLNPRDTLK"
     gene            1450..1782
                     /locus_tag="VGM72_12975"
     CDS             1450..1782
                     /locus_tag="VGM72_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266226.1"
                     /translation="MARMLPRSEMVAQAVGFVHDLYATVRFFVRYRTMRPLSLARRVE
                     AWLENRRGECVCDNCIAAELRQPIRKNVQAVTSRLSFCQPGYCKYRATCSVCGQASMV
                     TRVSTSML"
     gene            1883..2842
                     /gene="bla"
                     /locus_tag="VGM72_12980"
     CDS             1883..2842
                     /gene="bla"
                     /locus_tag="VGM72_12980"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF012229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallo-beta-lactamase"
                     /protein_id="HEY4266227.1"
                     /translation="MTTKRIAALALTTAAAAFLLASPVWAQAVYTGFPKSPTSPISQM
                     YREKGKADVEVQKVPPVKMFDNLWYVGPGYVSCYLIKTSAGAILIDASEEPDMVLHVI
                     DSIKKTGTDLKDIKAIFISHGHIDHWGGIDRMHELTGAPVYATEADWKMIEDTASKPG
                     RNGAPPPRAPKRDRIVHDGDTFTLGDTTLKLYVTPGHTPGVMSTEFTVFDNGVPHKAF
                     YSGGLGGRDGVKGFEDAVKSSARVATFQGIEVYLPNHGWNPGTDYPNGSLFERFPKLA
                     ARKPGEPNPFVDPASWKINIAQAQERIAKGLEEEKKKAAAAAK"
     gene            complement(2919..3500)
                     /locus_tag="VGM72_12985"
     CDS             complement(2919..3500)
                     /locus_tag="VGM72_12985"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEY4266228.1"
                     /translation="MLTKKILLAAGGMVLASWGAVELAHAATAPFRLMSSTFKDGTMM
                     PAKVANVSNPAMPNPNCVGQNVSPQLSWSGAPEGTKSFAIEMVDPEGRGGLGVFHWVA
                     YGIAPNVTSFAEGEVSKVSDKYIGGKSTQGVGSYSGPCTPPGSPHHYTFKVIATDLDP
                     KELPPGLTLPELDAKLAGHAKGAAGLVGLFVKP"
     gene            complement(3571..4539)
                     /locus_tag="VGM72_12990"
     CDS             complement(3571..4539)
                     /locus_tag="VGM72_12990"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY4266229.1"
                     /translation="MKTSAGILTLCLSSVAMAATAYAADLPRWAYPLNPPVVPGTQPP
                     KDDGTPKHVPGSNIALTRTQIAGRDGVPDWHPDDHPTMPDVVNKGRMPEVRACGYCHT
                     PSGAGRPENASLAGLTPAYIKQQVMNFRNSDRKGSEPKRVPQNLMMGIAQHVTDAEVE
                     QAAAYFSSIKPVSSVKIVEAGTVPKTTVVGGMMAKAPEGGMEPIAGRIIEVPDDTERA
                     ELRDDKTTFTAFVPLDALKKGEALVATGGNGKTLQCGICHGADLKGLGDVPRIAGRSP
                     SFLMRQLYDIQNGTRSGSAVLMKQVVAKLDNDDMVAIAAYVASRVP"
     gene            4839..5042
                     /locus_tag="VGM72_12995"
     CDS             4839..5042
                     /locus_tag="VGM72_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006470569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY4266230.1"
                     /translation="MTIGTVKFFNVNKGFGFISPEGGAKDVFVHATAVEAAGMRSLAE
                     GQRVSFDIQPDARGSKAVNLKSA"
     gene            complement(5070..5267)
                     /locus_tag="VGM72_13000"
     CDS             complement(5070..5267)
                     /locus_tag="VGM72_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266231.1"
                     /translation="MRFDILVASAARLFVNSDFTSVSNAAAFFSRTLAPPMFFVRIIS
                     SESLEGKWGSLEEKTGGCTFR"
     gene            5412..5564
                     /locus_tag="VGM72_13005"
     CDS             5412..5564
                     /locus_tag="VGM72_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266232.1"
                     /translation="MAMKPNYRQERLSRERTKDAKKQEKLRRREEESTKRKALRGDES
                     SEPEKS"
     gene            complement(5851..6231)
                     /locus_tag="VGM72_13010"
     CDS             complement(5851..6231)
                     /locus_tag="VGM72_13010"
                     /inference="COORDINATES: protein motif:HMM:NF040437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6481 family protein"
                     /protein_id="HEY4266233.1"
                     /translation="MKEFREPSFEDRRIAAAKARQAQLEKARANSPANDPNFAQKQAE
                     RLAASQAREARVAERKAQKLAEEQRRAAEKAAAEAARLAAEQEAAHARAVAAKEEADR
                     EVALIAARKAERDARYAARKARKK"
     gene            complement(6251..6427)
                     /locus_tag="VGM72_13015"
     CDS             complement(6251..6427)
                     /locus_tag="VGM72_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266234.1"
                     /translation="MNAVVFVTLDLFIAFVFVYIAARMANKRGRSARGWMWAAVISGP
                     LAISVLAMLSKKSD"
     gene            complement(6482..7171)
                     /locus_tag="VGM72_13020"
     CDS             complement(6482..7171)
                     /locus_tag="VGM72_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266235.1"
                     /translation="MRKSDFVRLAVMAALAATPAIMVSAISAAQAPAAPNPLPVIAPL
                     LPPSKTVDLATSEGMAAFSAQWKNMDAKIIEVPAMPDAGPAWKMTYDLTPKAGEANFN
                     DSAWPTIEPKGILERRGGGHLFMTWFRSNLTVPAKIGDFDTTGARMAISFTIDDYAEA
                     WVNGQMPRAAGRPSPATIQGFNMPNRVMLSESVKPGDKFQIAILGINGPISLAPANPV
                     FFREAKIEFFK"
     gene            complement(7345..7929)
                     /locus_tag="VGM72_13025"
     CDS             complement(7345..7929)
                     /locus_tag="VGM72_13025"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4266236.1"
                     /translation="MESFFATELDDFATGKLSRRALLETLTLAATTTYAAAPASAAMA
                     PDPALKVTLVNHISYNCPDFKKAADWYSKVFNLDQVGASKIDVALPFGKKGEKPMGVT
                     ANDVPLTHLIIRSRDLNAPAEGGAPRRKSQAVMEHIGYTVADFDREKAKAELKALGVE
                     NVRDGGLYSLHMTDAYGYDVQISGVANNALSDGA"
     gene            complement(7934..8473)
                     /locus_tag="VGM72_13030"
     CDS             complement(7934..8473)
                     /locus_tag="VGM72_13030"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4266237.1"
                     /translation="MEKFIDRLIKQFESGQIGRREFCQTLAMATAVFAGGAQANAAPA
                     GRGFKMLGLNHLSYQCSDYKKARDFYSSMFGMQVLNDKGKGRANLAFGPEPGKGGNFL
                     VVHNPGANPLKPSEAVIDHIAYTISNYDEGKVRAALKARGYDAPTGRDGSRHVYDPFD
                     YDVQFASAAQENAFRREGR"
     gene            8682..9545
                     /locus_tag="VGM72_13035"
     CDS             8682..9545
                     /locus_tag="VGM72_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266238.1"
                     /translation="MSIVSAAGMEPSAAIPALSGQWGRTNFNLEQPASGPTIISNTLR
                     KRDGTIDDDTARVGDFTNPILKPEAAEILRKHGEFSRTGQSIPDPHNQCWSEAPPFTL
                     TIQLEVLLLQQKDEVTLIYVNDHKVRHIRMNAAHPAHVTPTWQGDSVGHYEGDTLVVD
                     TVAIKPSPWPVVDRYGTPHSDALHVVERYRLIDGEAAAEAIRKHRRSFTAAPAGRFDG
                     YGAEFDPDTRHKGLQVEVTVEDPKMFTAPWKGLVTYRPETGWPEMVCAENTRELTGPE
                     RAVPTAVKPDF"
     gene            complement(9550..10326)
                     /locus_tag="VGM72_13040"
     CDS             complement(9550..10326)
                     /locus_tag="VGM72_13040"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY4266239.1"
                     /translation="MIVSRRHSLLTLSAAAMFPAASRAAVSSANVKPAKPIRLVVLDV
                     GGTLIEDHGEVPQAMHSALTKRGVEVSFAEIGEWRGASKRGMVRHFVELRIKPAADRE
                     ALIAAIYDDFSAQVNLAYANVRAIAGAEDCLRRLRSAGLLVATSTGFGRPLTDKIFAH
                     LGWRDYFVATVTSDDVVDGRPSPFMLFHAMEAAHVDAVAQVVAVGDTPLDLQAGINAG
                     MRGVVGVYSGAATEARLRQETYTHILPSVAALPDLLHAEF"
     gene            complement(10348..11367)
                     /locus_tag="VGM72_13045"
     CDS             complement(10348..11367)
                     /locus_tag="VGM72_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266240.1"
                     /translation="MKQHLRWVAYALAAAMLVTSQAQAATPIAFYRGRNLFVVIGYTP
                     GGGYDLYARLLARHLGRHTPGNPTVVPQNMPGAGSLKAANYLYTIAPKDGSVIGTFAH
                     GMGSAPLLGQAKFDARKFAWLGSITKDVMLCISWNGSPIRNWNDVMARQFLFGGVAAG
                     NAPDIYAKLYNSVFGAKIRLATGFPGTNDIALAMQRREVDGLCGIAWSTVKAQHGDWV
                     RDGKVKVLLQAAAAKAPDLRDVPLAGEFVRTRRGRQILDFVLASQVMARPFAAPPGIP
                     PERQAVLRAGFDETMKDPAFLADAKKLMLDVDPVSGQAIDTLIARLYATPKDIVAEAT
                     SVLVN"
     gene            11480..12214
                     /locus_tag="VGM72_13050"
     CDS             11480..12214
                     /locus_tag="VGM72_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266241.1"
                     /translation="MTHKSESVIPRLDELVHDYVRRTRFFHEPITMGRARAWVMQHRL
                     NTRQRNSVLKLRVATNCPDWDTRLQIIEACADEVIADEKYGRGKPHWQHLEELGVTLG
                     MRIEDVRAARPLDSTLLCWSAWDGLMSNTHWLEGVIANTCAERPNVPGYGIGAYREYG
                     RSGAERRRWRETFALNEDQLVWFAMHEAADLEHSNAGWNTIARYAKDLHMEDAVVEAC
                     RKNLLVWTLYFDGIREAGDTLEREFR"
     gene            12234..13277
                     /locus_tag="VGM72_13055"
     CDS             12234..13277
                     /locus_tag="VGM72_13055"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY4266242.1"
                     /translation="MHRRTFLATAAGAAIASAASGRETAAGELPLGPLPGSRYPDSHI
                     EALDRRFKYKIGNAAVERVAAGLRWCEGPVYFRDGGYLLWSDVPNNRIMRWSEDDGHL
                     SVYRSPSNNSNGNTRDREGRLITCEHDSRRVTRTEHDGSITVLMDKFEGKPLNSPNDV
                     VVSADGAIWFTDPLFGNAGNYEGHRITPELPTQVYRLDPKTGRASVAASGFGGPDGLA
                     FSPDEKKLYIVDTGVQTSIGRDSHVRVFDVNGERLTNDRIFIPAFGAVGRTDGARTDI
                     DGNLWLTLGSGDPNESGVRCYAPNGDLIGKIHIPETCANLCFGGKKKNRLFICGSTSI
                     YAVYLDTVGAMVP"
     gene            complement(13400..14797)
                     /locus_tag="VGM72_13060"
     CDS             complement(13400..14797)
                     /locus_tag="VGM72_13060"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4266243.1"
                     /translation="MQCKSAFKASCFGLAGLFALTGAASAQVGGPGQPWRGAGPQPCF
                     GAIDAGANKCPPAAETTAVRAGHLFDSKTGQMLANQVVLLNGDRIAEVGPAAQVKIPA
                     GAVVIDLSRETVLPGLIDAHTHMFNTPKPGMSREMSTLIAIQNTQADLRGGFTAIRDM
                     STHGNGYADVDIRNAINKGLIDGPRQQVSTRGIVWGPPGDNKPANPLASAVVHNVDEA
                     RAAVRDQIEHGADWIKLFPTGAYSFAPDGKQQFVLTYPMPVLQALIDETHRLGHKTAC
                     HVYGGEGEKNAIVAGCDSIEHAWGLDEEQAKMMVAKGLTYDPTLQRYTEPYMDDNDKK
                     NTGGKYRMIPTINNAVVIASKTPGLKITVGSGVDGATFPHGSQALEYVALVKQAHMSP
                     ARVIQAGTMNNAEILGWDKDIGSVEKGKYADLVAVPGDPLADITELTRVNFVMKGGKI
                     IRDDLMPRATETASR"
     gene            14960..15367
                     /locus_tag="VGM72_13065"
     CDS             14960..15367
                     /locus_tag="VGM72_13065"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY4266244.1"
                     /translation="MLDLSSLKPGLKGTDELKVRVEHTAPSIGSGLVPVLATPVMINL
                     IEAAALDAAERFLPEGHQSLGTHLDIRHIAATPIGMRVRASAEVTAVEGRNVSFRVEV
                     HDEKELIGGGTHQRVVVNVARFDERVKKKLLAP"
     gene            15437..15868
                     /locus_tag="VGM72_13070"
     CDS             15437..15868
                     /locus_tag="VGM72_13070"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY4266245.1"
                     /translation="MVQRTSQAEWRGNLQDGAGTMKLGSGAYEGAYSFKSRFADGTGT
                     NPEELIAAAHAGCFSMAFSAGLSKAGFTPTRVHTNATVTFDKTPAGFAVTRIDLDTEG
                     EVPGIEDDAFHQQAENAKRDCPISRLLAPGTEITMKARLIR"
     gene            16029..16250
                     /locus_tag="VGM72_13075"
     CDS             16029..16250
                     /locus_tag="VGM72_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266246.1"
                     /translation="MASITMSASEIAKLLDEAAAARQLAAIVEDSAAVDDLLDYAFAL
                     EACAAGERSVPPAQPDWLSQRARNHKADH"
     gene            complement(16271..16843)
                     /locus_tag="VGM72_13080"
     CDS             complement(16271..16843)
                     /locus_tag="VGM72_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266247.1"
                     /translation="MAKGKLAIFLCVLAISAGGRANTPSRAASPHECAGTPRDAVTTL
                     PAPLRKWAQIACTPYGDALGSRKGSIWAALDDARKVMIPSGDPQGNPDPVANETAYFT
                     AIDVSELKDDDRNFAVEIFHDGLDLDVANAKVYRVDLTSEAGGVNTIYFFDFDKFAGG
                     MWCPDGGCVPESRFLIMEQGPEAKSDWPSI"
     gene            17102..18346
                     /locus_tag="VGM72_13085"
     CDS             17102..18346
                     /locus_tag="VGM72_13085"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY4266248.1"
                     /translation="MASHSGDTAVASQPIRFSPHAKGPLAGIRVLDLSRLVAGNMLSL
                     QLGDFGAEVIKIEPLDGDPLRAWREGGEQLFWKTYSRNKMSVALDLRNAAAKAALLRL
                     VDTADVFIENYRPGTLENMGLSPDVLMARNPRLVVVRISGFGQTGPYAQLPGFGTLVE
                     AMSGLAARTGFADREPVLPPLALADMIAGLSGAMATVTALFARDRGTGKGQVIDLSLL
                     EPLFSTLGPEAAIYRQTGVVRERAGNGTSISSPRNIYLCADGKYVALSGSTQAMARRV
                     FETIGRADMIEDPRFRTNSDRVRNRARVDEAVGAWFTAKTRDEALAGMRSAGVSAGPV
                     YDIADAVNDPHFREREILVDVEDSELGALPMHNIVPRLSATPGVWRHPAPALGQHTDE
                     ILREAGLSASEIARLRAEGGCA"
     gene            18343..19695
                     /locus_tag="VGM72_13090"
     CDS             18343..19695
                     /locus_tag="VGM72_13090"
                     /inference="COORDINATES: protein motif:HMM:NF014697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HEY4266249.1"
                     /translation="MSAHNPPLAKRRSFASFSVDMFERWMPDAFVLAIALTAIVAGAA
                     AIFATDGSITTILTGWYGGIFNILGFAFQMILILVSGHALAYSPPVQRGLKRLVLNIA
                     TPNQAVVVTFLIAALASWINWGFGLVIGAVLAREVAKQVRVDFAWLVAAAYSGFVIYD
                     SGPSGSIPLSQASPGNALNIVEKFTGHTLPYTQSIFTTLNLVPTLLVLLVMPFVLMLM
                     RPRAGEVQPFIPPAEAPIAEKSSAPSFARTLERSPLASLFLVAIGVAYVAVAFTSGRL
                     TLDANMVIFLFLIAGLALHGSPLNFSDAVKNAAKQTGSMILQYPIYGGIMGIMTATGL
                     AAQISSSIAASASAYSLPILSFLSSILISLVIPSGGGHWAVQGPFTIPAAAALNASVS
                     ATTMAVAMGEQVANMLQPFWALPVVAIAGIGIQRVLGFTIVTFMVAGLIYGAALLILI
                     "
     gene            19776..20768
                     /locus_tag="VGM72_13095"
     CDS             19776..20768
                     /locus_tag="VGM72_13095"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY4266250.1"
                     /translation="MSDSKFTRRQVNRGLAAGFAILASRGARAAEINLQHAHNLPVES
                     PLHKRATQLWTAVRNETGGRVDVQIVRDDSGLDRLVQGGIAFITLAGNNVSSLVPAAD
                     VQATPYGFRNPAQVYAALDGELGAYLRDELRAKGIYAIPNGCFENGMHQITSNSRPIR
                     TAADFRGLKIRIPGSPVYQDFFKSMGAEIVTLNLTRLYDGLKAGMAEAQDDPWDVIEL
                     FKLYEFQKYASATEHSWSGYNLLASAKVWQGLPADVQRVIEINTKKFVALQRADNDAL
                     NNALRADLTKRGMMFNEVDKASFRPTLTAFYPKWKQHIGTRAWDLLEAHVGHLG"
     gene            20782..21321
                     /locus_tag="VGM72_13100"
     CDS             20782..21321
                     /locus_tag="VGM72_13100"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEY4266251.1"
                     /translation="MGRIIAVSRSAAHSFSKPNEPMIQLVAGLGVADDAHLGETVQHL
                     ARVREDPTKPNLRQVHLIHCELFDELREAGFSVAPGEIGENVTTRGIDLLGLPAGTRL
                     HLGESAVVQVTGLRNPCRQLDRFQPGLMAAMLGRDGQGRITLKSGIMGIVLAGGHVRA
                     GDSIAVALPPEPHRPMTRV"
     gene            21353..23014
                     /locus_tag="VGM72_13105"
     CDS             21353..23014
                     /locus_tag="VGM72_13105"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY4266252.1"
                     /translation="MPTEPAPPANNVAVELVRALRELDVKYVFGVPSGGWVDYMEALR
                     QADGIEFVLTTHEGGAGMMAEVAGRLGPAPGVCFGTFGPGATNLATGVGEALLDRSPM
                     IAFTDEMPPAMRGRTTQMGIDHQALFRPLTKKTTRLEPSRVRTILFDAARIALQGRPG
                     PVHVGLPAGMSAELVVPENIAFAPPETPPHADSALLEQAAALFAASKKPILAVGLGAV
                     RAHAAAKIRSLAERHKLPVILTPMAKGMLAEDNPCYAGVLFHALSDMVGQTHAQADLV
                     IGIGYDPVEFNYESWLPKTAPLVSFDIAPADIDTNNHILAVDAVGDIDYSLDTLLALP
                     PSEKHWDLDALAKRRTAMFAKLAPSPKPSQGAALGPKAALGILREILPADGIMTCDVG
                     AHTHLIGQLWHTPAPGLQIMTNGWSTMGFGIPAAIAAKLMRPAQSVCAVVGDGGFLMT
                     AGELATAMRLNLKIMVLVLTDNDLALIRIKQEKKANPIYGTPVRKTGTIGGDNIFGVP
                     VRTAQDAAGLRDALQWAFAASGPAIVEAIVDSREYDGLVLKKDRQ"
BASE COUNT         4400 a         7297 c         7097 g         4375 t
ORIGIN      
        1 ctggcgttcg cccggccacg gacaggcctt tccgccctat tccgtcgagc caccgcactg
       61 gcccgcggtg tgcctggatt ggaatgacgc caaaacctat gtcgtctggc tgaacggcaa
      121 ggccatggcg gcgcgtccca ccctggtgga cgcgccctat cgccttccga gtgaagcgga
      181 atgggaatac gccgcgcgcg cgggcaccgt cacggcgcgc tggtggggcg atgcggtggg
      241 ctcgggccat gcgaactgca atggctgcgg cagcaaattc gactatcacg tgctggcgga
      301 cgtcgacagc ttcccgccca atccgttcgg gctttacggc atgctcggca atgcgtggga
      361 atggaccgag gattgctggc acgagagcta tgctggcgcg cctgccgacg gcagcgcctg
      421 gacgcttggc gccgattgca tcaagcatgt cgtgcgcggc ggatcgtggg acaatgtgcc
      481 ggtctttgtg cgcgccgcga cccgcagcgg cagcgcgccc aatggcggcg aattcgatta
      541 ttcgagtctg gcgggattcc gcgtcgcgcg gaccttgcct tgaccttgcg cggtcatttc
      601 agcgtatcgc gcggattgag atccaaggtc atgtccatga ccccaccctg gcttgcgacc
      661 tgccccaatg gcagagcgaa aggcgaggac agcagcaccg cattgcgcgc gctgagcgcg
      721 atggagcggt aggcgacatc ggcgatatag cgtttctggt cgacgatctc ggccttggtg
      781 acggtgccgt cggcggccag caccacatgg atgggaatgg tgaaattgcg cgcgcccaat
      841 tcttccgtat tcagattcca gcgtcgttcg acctgggcgc ggatgtagtc cttcacgcta
      901 taggcggcat agggaccggg cgcgccattg ccgttcgcat tgatgccggc ggtccctgct
      961 ccgttcaaaa gacgcgggtc ggtggagggc tgtttcaaat tggcaaggcc ctggagcttg
     1021 gtttccagtt cgtcgcgcgg ctgttcgggc ggggccgtct cggccggcgg attgggaatg
     1081 gcgggccgct caatggtgcg ggcttgaggc gcgcgcggcg gcgtgagaga ggcctgccgc
     1141 tggcgggtgg cggcctgggg ccgctgcggc agagcccggt cgcgcgatgg ggccggggcg
     1201 atggtttctt cggcaagctg cacgagatcc acaggcacgg cgggcggtgg agagggtggc
     1261 agcgccgccc tcatgtgaaa gatgaggaaa aacgccacaa gaccatgaat tacaaaggaa
     1321 atagccaagc caggacgttt cgcataggaa gccaccccgt tcaccctgag gggaacggcc
     1381 tcgtctttct cgccaagcag actcgggagc atgatgcggc ctctgaggcc ccaagggcat
     1441 aacgcattaa tggcacggat gctgccccgt tccgagatgg tggcgcaagc cgtgggtttc
     1501 gttcatgatt tgtacgcaac cgtgcgtttc tttgtgcgtt accgcaccat gagacctcta
     1561 tccctcgcgc gtcgcgtcga agcctggctg gagaaccggc ggggtgagtg tgtttgcgac
     1621 aattgcatcg ccgcggagct gcggcagccc atccgcaaga atgtccaggc ggtgacaagc
     1681 cggctgtcgt tctgccaacc cggttactgc aaataccgcg cgacctgttc ggtgtgcggt
     1741 caggcgagca tggtcacgcg agtcagcacc agcatgctgt aaggcttttc caatcatcct
     1801 tcccgagaca tggcgctctg tattatagag agccaggctt cggtggcgca tgggcgtcag
     1861 ccagaggcga cgggaggcat ccatgacgac aaaacgaatc gcagcgctcg cgctgaccac
     1921 cgcggctgcg gctttcttgc tggcaagccc ggtctgggca caggccgtct acaccggctt
     1981 tcccaaaagc ccgacgagcc ccatcagcca gatgtaccgg gaaaagggca aggcggatgt
     2041 cgaggtgcag aaggtgccgc ccgtcaagat gttcgacaat ctctggtatg tcgggccggg
     2101 ctacgtctcc tgctatttga tcaagacctc ggccggcgcc atcctgatcg acgcgtcgga
     2161 ggagcccgac atggtgctgc acgtcatcga cagcatcaag aagaccggca ccgacctgaa
     2221 ggacatcaag gccatcttca tcagccacgg ccatatcgat cattggggtg gaatcgaccg
     2281 gatgcacgaa ctgaccggcg cgcccgtcta tgccaccgaa gcggattgga agatgatcga
     2341 agacaccgcc tcgaagccgg gacgcaacgg agccccgccg ccgcgcgcgc ccaaacgcga
     2401 tcgcatcgtg catgacggcg acaccttcac gctgggcgac acgacgctga aactgtatgt
     2461 cacccccgga cacacgccgg gcgtgatgtc gacggaattc accgtcttcg acaatggcgt
     2521 gccgcataag gcgttctatt ctggcgggct tggcggacgc gacggcgtga agggcttcga
     2581 agacgcggtc aaaagctctg cgcgcgtcgc cacattccag ggcatcgaag tgtatctgcc
     2641 gaaccatggc tggaatccgg gcacggatta tcccaacggc tcgctgttcg aacgcttccc
     2701 gaaactcgcg gcgcgcaagc ccggcgagcc caatccattc gtggacccgg cctcgtggaa
     2761 aataaacatc gcccaggcgc aagagcgcat cgccaagggc ctggaggagg aaaagaagaa
     2821 agccgccgcc gccgcgaaat aaccggcgca gcctttccgc gcaaaaacaa acgccctctc
     2881 cggccggaga gggcgtttcg cgtttcggac ttcgacgatt acggcttcac gaacagtccc
     2941 acgagaccgg ccgcgccctt ggcatgaccg gcgagcttgg catccagttc gggaagcgtc
     3001 agacccggcg gcaattcctt cgggtccaga tcggtcgcga tcaccttgaa ggtgtaatga
     3061 tgcgggctgc ccggcggcgt gcaggggccc gaatagctac ccacgccttg cgtgctcttg
     3121 ccgccgatat atttatcgga aaccttgctg acttcgcctt cggcgaagga cgtcacgttc
     3181 ggcgcgatgc cataggccac ccaatggaac acgccgagac caccgcgtcc ttcgggatcg
     3241 accatctcga tggcaaagct cttggtccct tccggcgcgc cgctccacga caattgcggc
     3301 gacacattct ggccgacgca attggggttg ggcatcgcgg gattgctcac attcgcgact
     3361 ttggccggca tcatggtgcc gtccttgaac gtgctggaca tcagcctgaa cggcgccgtg
     3421 gccgcatggg caagttcgac ggcgccccag gacgccagga ccataccgcc cgcggcgaga
     3481 aggattttct ttgtaagcat atgttttttc tccggttagg tttcgacctc atcatcggcg
     3541 ggaatttgct cccgccgcca atctctaatt tcagggtaca cggctcgcga cataggcggc
     3601 aatggcgacc atgtcgtcat tgtccagttt ggcgaccacc tgtttcatca acacggccga
     3661 tcccgaacgc gtgccgtttt ggatgtcgta aagctgacgc atcaaaaaac tcggactgcg
     3721 tccggcgatg cgcggcacat cgccgaggcc cttcagatcg gcgccgtggc agatgccgca
     3781 ttgcaaggtc ttgccgttgc cgccggtggc gacgagggct tcgcctttct tcagcgcgtc
     3841 caagggcacg aaggcggtga acgtggtctt gtcgtcgcgc agctcggcgc gttcggtatc
     3901 gtccggcact tcgatgatgc gacccgcgat cggctccatg cccccttcgg gcgctttggc
     3961 catcatgccg ccgaccaccg tcgtcttcgg aacggtgccg gcttccacga tcttcacaga
     4021 ggacaccggc ttgatagagg agaaataggc tgcggcctgt tcgacttcgg catccgtcac
     4081 gtgctgggcg atccccatca tcagattttg cggaacgcgc ttgggctccg aacccttgcg
     4141 gtcgctgttc cggaaattca tcacctgctg cttgatgtaa gctggcgtca gccccgcgag
     4201 gcttgcattt tcgggccgtc cggcgccgct cggcgtgtgg caatagccgc aggcgcgcac
     4261 ttcgggcatg cggcccttgt tcaccacatc gggcatggtg ggatgatcgt cgggatgcca
     4321 gtccggaacg ccatcacggc ccgcgatctg ggtgcgggtc aacgcgatgt tgctgcccgg
     4381 aacatgtttc ggtgtgccgt cgtcttttgg cggctgcgtg ccgggaacta ccggaggatt
     4441 gagcggatag gcccatctcg gaaggtccgc ggcgtaggcc gtcgcagcca tcgccacgga
     4501 tgacaggcac agcgtcaaaa tcccggcgga agtcttcatc agggcgctct tgttcacgga
     4561 ccggtttttc gacccggttc ggcgggcagg atggtttttt tcccactgtc tggcaagacg
     4621 gagccgttcc ggtttgggtt gtaatattgc gtcttaaggc ctatatcagc gtcgcgacgg
     4681 cgtccgtcag atgaagcgca ctgtatccac ccgacccgta cttcgtgcgc gctggacgag
     4741 acagccaaac ttcccgacct gatgtttgcg tcacgatcgc gcgcgcgagc acgtgctcaa
     4801 gcgtttatca gcgcgcgaaa gcgcacagga gcaatgtaat gactatcgga actgtaaaat
     4861 tcttcaatgt taacaaaggc tttggattca tttccccgga aggcggcgcg aaggacgtgt
     4921 ttgttcacgc caccgccgtc gaagcggccg gaatgcgcag cctcgcagag ggccagcggg
     4981 tgtcgttcga catccagccg gatgcgcgcg gttcgaaggc cgtgaatctg aagtcggcct
     5041 aaaccccgat ccgtctcaac gggcggctgt caccgaaatg tgcagccgcc tgttttttct
     5101 tcaagcgatc cccatttccc ttcaagcgat tccgaggaga tgatgcgaac aaagaacatc
     5161 ggcggggcca gcgtgcggga aaagaaagcg gcggcgttgg acacgctcgt gaagtcggaa
     5221 ttgacaaaga gccgcgcggc ggaggcaacc aaaatgtcga acctcaaggc gcttcgcctt
     5281 gccaaagagg ccgaacagcc cgatccggcg ctcgaaaaca ctgacgaaaa gatcaaattc
     5341 ctgccgcgca aacctcgccg cgcgcgatcc ggctcttaag cgtaagctcg cgcaacggaa
     5401 aaggacacgc catggcgatg aaacccaatt accggcagga gcggctcagc cgcgaacgca
     5461 ccaaggatgc gaagaaacag gaaaagctgc ggcgccgcga ggaggaatcc accaagcgca
     5521 aggctctgcg cggcgacgaa tccagcgaac ccgagaagag ctaaggtcgc tcggattctc
     5581 aatccgtcat ggcccgccaa aggggccatc caagataccg aggattggtt gctacgcttt
     5641 gtgcgtcccc tcgtgccctt tgtgtctgct ttggtccaat tgccacgcgc gcgagcaaca
     5701 tcgccgcacg gatgattgga tcggcctttg tttaggccag ccaaattcaa acacaaaaat
     5761 actaagaacc acgcaggctt acggccgcgc attcgcgtgg tgaatgagat gacccgcttc
     5821 gcgggccatg acagaccttt ccggatgggc ttatttcttt cgcgccttgc gggccgcata
     5881 gcgcgcatca cgttcggctt tgcgcgcggc tatcagagcg acttcgcggt cagcttcctc
     5941 tttcgcggcg acggcacggg catgcgcggc ttcctgttcg gccgccaggc gtgcggcctc
     6001 agccgcggcc ttctcggcgg cgcggcgctg ttcttcggca agcttctgtg ccttgcgttc
     6061 ggccacgcgc gcctcgcgcg cctgactcgc ggcaaggcgc tctgcttgct tttgggcgaa
     6121 attcggatcg ttcgcgggag agttcgcgcg cgccttttcg agttgagcct gccgggcctt
     6181 ggccgccgcg attcgccggt cctcaaagct cggttctcgg aattccttca tgtcgtgcgc
     6241 ctcggattaa ctagtctgat tttttggaca gcatcgccag caccgaaatg gcgagcggtc
     6301 cagaaatgac cgccgcccac atccagcctc tggcggacct gccccgttta ttcgccatgc
     6361 gtgccgcgat gtaaacgaag acaaacgcaa tgaaaagatc gagcgtgacg aaaacgaccg
     6421 cgttcatgcc ggccccttac catgattaca gccatttttc acaataatgg ccggtcttcg
     6481 cttatttgaa gaattcgatt ttcgcttcgc ggaaaaaaac cgggttcgcg ggcgcaagtg
     6541 aaatcggccc gttgatgccc agaatcgcga tctggaattt gtcgccgggc tttacgctct
     6601 cgctgagcat gacgcggttc ggcatgttga agccctggat ggtggccggg ctcggacgcc
     6661 ccgccgcgcg cggcatctgg ccgttgaccc aggcctccgc gtaatcgtcg atggtgaagg
     6721 agatcgccat ccgtgcgccg gtggtgtcga agtcgccgat cttggcgggg accgtcaggt
     6781 tcgaacgaaa ccaggtcatg aacaaatgtc cgcccccacg ccgctccagg attcctttgg
     6841 gctcgatggt cggccatgcg gaatcgttga agttcgcctc ccccgccttc ggcgtgaggt
     6901 cgtaggtcat tttccacgcc gggccggcat cgggcatcgc gggcacctcg atgatcttgg
     6961 cgtccatgtt cttccattgc gcgctgaagg cggccatccc ctcagacgta gccagatcga
     7021 cggttttcga cggcggcagt aacggcgcga tgacgggaag cgggtttgga gcggccggcg
     7081 cctgcgcggc ggatatagcc gaaaccatga tggccggcgt cgcggcgagc gcggccatga
     7141 cagccagcct gacgaaatcc gatttgcgca ttttgccctc ccatgggttg ttctttgcgc
     7201 gatcctaccc cgatagacgt tgaaaaacga gcgcggcgcg gagagtttgc aacatccatg
     7261 agcgaaatca ccgccgtgcc ggaccgtcaa tgaaaaggcc ccgccggtga aagcggggcc
     7321 ttcgcatcga tttgaacgcg gcccttacgc gccgtcggac agcgcgttgt tggcgacgcc
     7381 gctgatctgc acgtcgtagc cataggcgtc cgtcatgtgc agactgtaga ggccgccgtc
     7441 gcgcacattt tcgacgccga gcgccttcag ctccgctttc gccttttcgc gatcgaaatc
     7501 ggccaccgta taaccgatat gttccatgac cgcctgactc ttgcggcgcg gcgcgccacc
     7561 ttcggccggc gcgttcaggt cgcgcgagcg aatgatgaga tgcgtcagcg gcacatcatt
     7621 ggccgtgact cccatcggct tctcgccctt tttcccgaag ggcagcgcga cgtcgatctt
     7681 cgaagcgccg acctgatcca ggttgaacac tttcgaatac cagtccgcgg cctttttgaa
     7741 gtccgggcaa ttgtacgaga tgtgattgac caacgtgact ttgagtgccg gatcgggcgc
     7801 catggcggcc gaggcaggcg ccgcggcata ggtggtcgtg gcggccagcg tgagcgtctc
     7861 gagcagagcg cggcggctga gcttgccggt cgcgaaatcg tcgagctcgg ttgcgaagaa
     7921 gctttccatg accttatctc ccctcacgcc ggaatgcgtt ttcctgtgcc gcactggcaa
     7981 attgcacatc gtaatcgaac ggatcgtaga cgtggcggct gccgtcacgt ccggtcggcg
     8041 catcgtagcc gcgcgctttc agcgccgctc tcaccttgcc ttcgtcatag ttcgaaatcg
     8101 tataggcgat gtggtcgatc acggcctcgc tcggcttgag cgggttggcg cccggattgt
     8161 gtacgacgag gaagttgccg cccttgcccg gttccggacc gaaggcgagg ttggcgcggc
     8221 ccttgccctt gtcgttcagg acctgcatgc cgaacatcga ggaatagaaa tcgcgtgcct
     8281 tcttataatc ggagcactga taggacagat gattaagccc aagcatcttg aagccccgcc
     8341 cggcgggcgc cgcattggct tgtgcaccgc cggcgaacac cgcggtggcc atggcaagcg
     8401 tctgacagaa ttccctccgg ccgatctgac cgctttcgaa ctgcttgatg agccgatcga
     8461 tgaatttttc cactcccctg accctccctt gtgaaatcga ttcaggcccg aggcggaccg
     8521 tcgatctgcc gccagcatag cgattttatg ccgccggaga cagatggctg atggaaggcc
     8581 gtgcgccatg aggttagtat gcgccaccca accgcaaagg agcgactgtg cggagcattt
     8641 tggcaaagca tctccggcgg accgcgacgg cggcaatggc gatgagcatc gtatccgcag
     8701 cgggcatgga gccctccgca gccatccccg cgctctccgg ccaatggggc cgcacaaatt
     8761 tcaatcttga acagccggct tcagggccca ccatcatcag caatacgctt cgcaagcgcg
     8821 acggcaccat cgacgacgac accgcgcgcg tcggtgactt caccaatcct atcctcaaac
     8881 ccgaggccgc cgaaatcctg cgcaagcacg gcgaattttc ccgcaccggc cagtccatcc
     8941 ccgacccgca caatcaatgc tggtccgaag cgcccccctt cacgctgacc atccagttgg
     9001 aggtccttct gttgcagcag aaggacgagg tcacgctcat ctatgtgaac gaccacaagg
     9061 tgcgtcacat ccgcatgaac gcagcgcacc ccgcccatgt gacgcccacc tggcaaggcg
     9121 attccgtcgg ccactatgaa ggcgacacgc tcgtcgtcga taccgtcgcc atcaagccca
     9181 gcccctggcc ggtcgtggac cgctatggaa cgccgcacag cgacgcgctg cacgtggtgg
     9241 aacgctatcg tctgatcgat ggcgaggccg ccgccgaggc gatccgcaaa caccgccgca
     9301 gcttcaccgc ggcccccgcc ggccggttcg acggctatgg cgcggaattc gatccggaca
     9361 cccgtcacaa ggggttgcag gtcgaagtca ccgtcgaaga tcccaagatg ttcaccgcac
     9421 cgtggaaagg gctcgtcacc tatcgccccg aaacgggctg gccggaaatg gtgtgcgccg
     9481 aaaacacccg tgaattgacc ggcccggaac gcgcggttcc taccgccgtc aagccggatt
     9541 tctgaattct tagaattcgg cgtgcagcag atcgggtaat gccgccacgc tgggcaggat
     9601 atgggtatag gtttcctgcc gcagccgtgc ctcggtcgcc gcccccgaat agacgccgac
     9661 cacaccgcgc atgccggcat tgattccagc ctgaagatca agcggcgtgt cgcccaccgc
     9721 gaccacttgc gcgacggcat cgacatgagc cgcctccatc gcgtggaaca gcatgaaggg
     9781 cgacggacgc ccatccacca catcgtcgct ggtgacggtc gcgacgaaat aatcccgcca
     9841 gccgagatgc gcgaaaatct tgtccgtgag cggacggccg aagcccgtgc tggtcgcgac
     9901 cagaagtccc gcgctgcgca atctccggag acagtcttcg gcgcccgcga tcgcgcgcac
     9961 attcgcatag gccagattca cctgtgcact gaaatcatcg tagatggcgg cgatcagcgc
    10021 ctcccgatcg gcagcgggtt tgatccgcaa ttcgacgaag tgacgaacca tcccgcgctt
    10081 ggacgcgcca cgccattctc cgatctcggc gaagctgacc tccacaccgc gtttggtgag
    10141 cgcgctgtgc atggcttgcg gcacctcgcc atgatcctcg atcagggtgc cgccgacatc
    10201 gagcaccacg agacggatgg gtttcgcggg cttcacattg gcgctggaaa ccgcggcgcg
    10261 cgatgcggcg gggaacattg cggcggcgct cagcgtgaga agcgaatggc ggcgggaaac
    10321 gatcacatcg atctcctgaa aatgcgctca gttcacgagc acgcttgtgg cttcggcgac
    10381 aatatccttg ggcgttgcat aaagcctcgc aatgagagtg tcgattgcct gcccgctcac
    10441 cggatcgaca tccagcatca gttttttcgc atccgcgaga aaggcagggt ccttcatggt
    10501 ctcatcgaag cccgcccgca agacggcctg gcgctcgggc ggtatgccgg gcggcgccgc
    10561 aaaaggacgc gccatgactt ggctcgcgag gacgaaatcc agtatctgcc gtccccgccg
    10621 tgtacggacg aattctccgg cgagcggaac atcgcgaagg tccggggcct tcgcggccgc
    10681 cgcctgtaaa agcaccttga ccttcccgtc gcgcacccaa tcgccgtgct gggccttcac
    10741 cgtgctccag gcgatgccgc aaagtccgtc cacctcgcgg cgttgcatgg cgagcgcgat
    10801 atcgttggtg ccgggaaagc cggtggccag tctgatcttg gccccaaaga cagaattgta
    10861 gagtttggca tagatgtccg gcgcattccc cgccgctacg ccgccaaaga ggaattgcct
    10921 ggccatgaca tcgttccaat tcctgatcgg cgagccgttc caggaaatgc aaagcatcac
    10981 atccttggtg atgctgccga gccaggcgaa tttccgggca tcgaactttg cctgcccgag
    11041 caagggtgcg cttcccatgc catgcgcgaa agtgccgatc acgctgccat ccttgggggc
    11101 gatcgtataa aggtaattcg ccgccttcag tgagcccgca ccgggcatgt tctgcggcac
    11161 gacggtcgga ttgcccggag tatgccgccc cagatggcgc gccagcagac gcgcgtagag
    11221 atcatagccg ccgcccggcg tgtagccgat caccacgaag agattgcggc cgcgatagaa
    11281 tgcgattggc gtcgcggctt gcgcttgcga tgtgacaagc atcgccgcgg cgagcgcata
    11341 tgcaacccag cggaggtgtt gcttcatgtc caaaatccta gcacgctctg cctctgcccc
    11401 tgaaattgcc ccgctgaaat tgtcaccgga ggcgcaattg cctaatctcg cgcctttcga
    11461 accgaaacgg aaaatccccg tgacgcacaa atccgaatcc gtgatccccc ggctggacga
    11521 actcgtccat gattatgtgc gccgcacgcg ctttttccac gagcccatca ccatgggacg
    11581 cgcgcgcgcg tgggtgatgc agcatcgcct caacacgcgc cagcgcaatt cggtactgaa
    11641 gctgcgtgtc gccaccaatt gtccggattg ggacacccgt ttgcagatca tcgaagcctg
    11701 cgccgacgag gtgatcgccg acgagaaata cggccgcggc aagccgcatt ggcaacatct
    11761 ggaagaactt ggcgtcactc tcggcatgcg catcgaagac gtgcgcgccg cacgcccgct
    11821 cgattcgacg cttctgtgct ggtcggcctg ggacgggttg atgtcgaaca cacattggct
    11881 ggaaggggtg atcgccaata cctgcgcgga gcggccgaac gtgccgggct atggaatcgg
    11941 cgcatatcgc gaatatgggc gcagcggcgc cgaacgcaga cgctggcgcg agacattcgc
    12001 gctgaacgag gatcagcttg tgtggttcgc gatgcacgag gcggccgatc tcgaacattc
    12061 caacgccggc tggaacacca tcgcgcgcta tgcgaaagac ctgcacatgg aagacgcggt
    12121 ggtcgaagcc tgccgcaaga accttctggt ctggacgctc tatttcgatg gcatccggga
    12181 ggccggcgat acattggaac gcgaatttcg ataatacaaa aaagggggga gacatgcaca
    12241 gacgcacatt tctggcgacg gcagcgggag ccgcaatcgc ctccgcagca tcgggacggg
    12301 agactgccgc cggcgaattg ccgctgggac cgttgcccgg ctcgcgttat cccgattccc
    12361 acatcgaagc gctggaccgg cgcttcaaat acaagatcgg caacgcggcc gtcgaacgcg
    12421 tggccgccgg tttgcgctgg tgcgagggcc cggtctattt ccgtgacggc ggctatcttc
    12481 tgtggagcga cgttccgaac aatcgcatca tgcgctggag cgaggatgac ggacatcttt
    12541 ccgtctatcg cagtccgtca aacaattcga acggcaacac gcgcgatcgc gagggacgcc
    12601 tcatcacctg cgaacatgat tcacggcgcg tgacacgcac ggaacatgac ggctccatca
    12661 ctgtcctgat ggacaaattc gaaggcaagc cgctgaattc ccccaacgat gtcgtggtgt
    12721 ccgccgacgg cgcgatctgg ttcaccgatc ctctgttcgg caatgccgga aattatgagg
    12781 ggcaccgcat cacccccgaa cttccgaccc aggtctaccg cctcgatccg aagaccggcc
    12841 gcgccagcgt tgcggcgagc ggtttcggcg ggccagacgg tcttgccttt tcacccgacg
    12901 agaaaaagct ctacatcgta gacaccggcg tacagacctc tatcggccgc gattctcatg
    12961 tgcgcgtctt cgacgtcaac ggcgaacggc ttaccaatga caggattttc attccggcgt
    13021 tcggcgcggt tgggcgcacc gatggtgcgc gcacggacat cgacggcaat ctctggctca
    13081 ctctgggaag cggcgatccg aacgaatccg gcgtgcgctg ttatgcgccc aacggcgatc
    13141 tgatcggcaa gatccacatc ccggagacct gcgccaatct ttgtttcggc ggcaagaaga
    13201 agaaccgtct cttcatctgc ggcagcacgt caatctatgc ggtttatctc gatacggtcg
    13261 gcgcgatggt gccgtgacat cctgtgtcgc cctccctcaa gaggaggatc gcggcgattg
    13321 agagacttac gcgcgcgggc ataacgaatg agtggccggg ccagaccccg gccactcgga
    13381 aacagcttag tcgatgggac tatcgcgaag cggtctccgt cgcgcgcggc atcagatcgt
    13441 cgcggatgat cttgccgccc ttcatcacga aattcacgcg ggtaagctcg gtgatgtcgg
    13501 cgagcggatc gcccggcacc gcgaccagat cggcatattt gcctttctcc accgacccga
    13561 tgtccttgtc ccatccgaga atctcggcat tgttcatggt gcccgcctgg atgacacgcg
    13621 ccgggctcat atgcgcctgc ttgacgaggg ccacatattc gagcgcctgg ctgccatgcg
    13681 ggaaggtcgc gccgtccaca ccgctgccca ccgtgatctt caggcccggc gtcttcgagg
    13741 cgatgacaac ggcgttgttg atggtcggga tcatgcgata tttgccgccg gtgttcttct
    13801 tgtcgttgtc gtccatatag ggctcggtgt agcgctggag cgtgggatcg taggtcaatc
    13861 ccttggcgac catcatcttc gcctgttcct cgtcgaggcc ccaggcgtgc tcaatggaat
    13921 cgcagcccgc gacgatggcg ttcttctcac cctcgccgcc atagacatgg caggccgtct
    13981 tgtggccaag ccggtgcgtt tcatcgatca atgcctgcag caccggcatc ggataggtga
    14041 gcacgaattg ctgcttgccg tcgggcgcga aggaataggc gccggttgga aacagcttga
    14101 tccaatcggc accatgttcg atctggtcgc gtaccgccgc gcgtgcttcg tccacattgt
    14161 ggaccaccgc actcgccaag ggattggcgg gcttattgtc gccaggggga ccccagacga
    14221 taccgcgcgt ggacacctgt tgccggggcc catcgatcag gcccttgttg atcgcgttcc
    14281 ggatgtcgac atccgcatag ccattgccat gggtgctcat gtcgcggatc gcggtgaagc
    14341 cgccgcgcaa atcggcctgc gtgttctgga tcgcgatcag cgtggacatt tcgcgcgaca
    14401 tgccgggctt gggcgtgttg aacatatggg tgtgcgcgtc gatcagaccg ggcagcacgg
    14461 tctcgcggct gaggtcgatc accacagccc ctgcgggaat tttcacctgc gccgcggggc
    14521 ccacttcggc gatgcggtcg ccgttgagca gcaccacctg attggccagc atctggccgg
    14581 ttttgctgtc gaacagatgg cccgcgcgaa ccgccgttgt ctcggcggcc ggtggacatt
    14641 tgttggcgcc cgcgtcgatg gccccgaagc agggttgcgg gcccgcgccg cgccagggct
    14701 gtcccggtcc gccgacttgc gcggacgccg cgcccgtgag cgcgaacagt ccggccaaac
    14761 caaaacatga cgccttgaac gcagatttgc actgcatcgc ataatcctcc atcgatgaga
    14821 atcgagccgg tccctcccgg agaaccctcg aggcgccaat ataatcgaca taaaggccca
    14881 gcgacagcat gttgcggtga cctgacccgt gcacctctgc cataaggcga gggatgacgc
    14941 cgcctcctgg gacctgacca tgctcgacct ctccagcctc aaaccaggcc tcaaaggaac
    15001 cgacgagcta aaggtccggg tcgaacacac tgcgcccagt atcggcagcg gcctggtgcc
    15061 ggtcctggcc acgccggtca tgatcaatct catcgaagcc gcagcgctgg acgccgccga
    15121 acgcttcctg ccggaggggc atcagagcct cggcacccat ctcgatatcc gccacatcgc
    15181 agcgacgccc atcggcatgc gcgtgcgtgc gagcgccgag gtgaccgccg tcgaaggacg
    15241 caacgtctcc ttccgcgtcg aagtgcacga cgagaaggaa ctgatcggcg gaggaaccca
    15301 tcaacgggtg gtcgtcaatg tcgcgcgctt cgatgagcgg gtgaagaaaa agctgctcgc
    15361 gccataaata atcggtccga ttgccgcggc ggcgataagg cgtcaataat gcgcgcattc
    15421 atccaaggag gacctcatgg ttcagcggac atcgcaagcg gaatggcgcg gcaatttgca
    15481 ggacggcgcc gggacgatga aattgggcag cggcgcctat gaaggcgcct attcgttcaa
    15541 gtcgcgtttt gccgacggta cgggcacaaa tcccgaggag ctgatcgccg ccgctcatgc
    15601 cggctgcttc tccatggcgt tctccgccgg attgtcgaag gccggtttca cgccgacgcg
    15661 tgtgcacacg aacgcgactg tgaccttcga caagacccct gccggctttg cggtcacacg
    15721 catcgatctc gacaccgaag gcgaagtgcc gggcatcgaa gatgacgcgt ttcatcaaca
    15781 ggcggagaac gccaaacgcg attgcccgat ctcgcggctg ctggcgcccg gaaccgagat
    15841 caccatgaag gcgcgcctga tccgctgagg gtcaagtgcc gcgcgcagac agaccggatt
    15901 tctgaaaatc caagccgttc cggcattaaa cattgcggcg caattaaccg gttttccaat
    15961 cgccgattgt gggcctgggc gcgctctgca agcctcgtga ggactttccc cacggagcgg
    16021 ggcccgcgat ggcttccatc acaatgagcg caagcgagat tgccaaactc ctcgatgaag
    16081 ccgctgccgc gcggcagctt gcggcgatcg tcgaggactc cgccgcggtg gacgatttgt
    16141 tggactatgc cttcgccctc gaagcctgcg cggcgggcga acgcagtgtt ccacccgccc
    16201 aaccggattg gctatcgcaa cgcgcgcgga accataaagc cgatcactga atccgtcgct
    16261 gttcgttgcg tcagatcgac ggccagtccg acttcgcctc cggtccctgt tccatgatca
    16321 ggaagcgcga ttccggaacg cagccgccat cgggacacca catgccgccg gcgaatttgt
    16381 cgaagtcgaa gaaatagatc gtgttgaccc cgcccgcctc agaggtgaga tcgacccgat
    16441 agaccttcgc attcgcaaca tcgaggtcga ggccgtcgtg aaagatttcc accgcgaagt
    16501 tgcggtcgtc atcttttaat tcgctcacgt cgatggcggt gaaataggcg gtttcattcg
    16561 ccacgggatc gggattaccc tgcgggtcgc ctgacggaat catgactttc cgggcgtcgt
    16621 cgagagcggc ccagatcgag cccttgcggc tgcccaaggc atcgccgtaa ggggtgcagg
    16681 cgatttgcgc ccatttgcgc aaaggcgcgg ggagtgttgt gacggcatcg cgaggagttc
    16741 cggcgcattc gtgcgggctt gcggctctag aaggggtatt tgccctgccc cccgctgaaa
    16801 tcgccaacac acaaaggaag attgcgagct tccccttcgc catgacattc actccttaaa
    16861 ccctgttggt catcgccatc gtctggatac gcaccacaat tgtttcaaat cacaggacga
    16921 aggctacgcg tcaaagacct aagaatgcat tgatagtggg cgcgtgaatg tcgcaggccc
    16981 atacgtgaac ctacgtagat cgcatcaccc ttcattgccg aatgtgacga acgtgcccct
    17041 cgccgcggtt tcatgctatc agcggattga cgtcctcaaa cattgagaca acgggcaaat
    17101 cgtggcttcc cattccggcg acacagccgt tgcatcccag cccattcgct tttcgccgca
    17161 cgcgaaggga ccgcttgcgg gcattcgggt gctcgatctt tcacggctgg tcgcgggcaa
    17221 catgctgtcg ctgcagctcg gcgatttcgg cgccgaggtg atcaagatcg agccactcga
    17281 tggcgatccg ctgcgcgcct ggcgcgaagg aggcgagcag cttttctgga aaacctacag
    17341 ccgcaacaag atgtcggtgg cattggacct tcgcaacgcc gcggcaaaag ccgcgctcct
    17401 gcgcctcgtg gacacagccg atgtgttcat cgaaaattac cgccccggca cgctggagaa
    17461 catggggctt tcgcccgatg tgctgatggc gcgcaacccg cgtctcgttg tcgtgcgcat
    17521 ttccggcttc ggccagacgg gaccctatgc gcaattgccc ggtttcggca cgctcgtcga
    17581 agcgatgagc gggcttgccg cgcgcaccgg ctttgccgac cgcgagccgg tgctgccgcc
    17641 gctcgcgctg gccgacatga tcgccggcct gtccggggcc atggcgaccg tgaccgcgct
    17701 gttcgcacgc gaccgcggca ccggcaaagg ccaggtgatc gatctgtcgc tgctggaacc
    17761 gctcttttcc acgctggggc cggaggccgc gatctaccgg cagaccggcg tggtcaggga
    17821 gcgcgccggc aatggcacga gcatttcatc ccctcgcaat atctaccttt gcgcggacgg
    17881 caaatacgtt gccctctccg gctcgaccca ggcgatggcg cggcgcgtgt tcgaaaccat
    17941 cggccgcgcc gatatgatcg aagacccgcg cttccgcacc aacagtgacc gcgtgcgcaa
    18001 tcgcgcaaga gtcgatgagg cggtcggcgc ctggttcacc gccaagacgc gcgacgaggc
    18061 gctggccgga atgcgcagcg cgggcgtctc ggccggtccc gtctacgaca tcgccgacgc
    18121 ggtgaacgac ccccatttcc gtgaacgcga aatcctggtc gatgtcgaag attcggagct
    18181 gggcgcactc cccatgcaca acatcgtgcc acggctttcg gcgacgccgg gcgtgtggcg
    18241 gcatcccgcc cccgcactcg gtcagcacac ggatgagatc ctgcgcgagg ccggtttaag
    18301 cgcaagtgaa atcgcgcgat tgcgagccga aggcggctgc gcgtgagcgc gcacaatccc
    18361 ccgcttgcca aacggcgaag cttcgcctcg ttttccgtcg atatgttcga gcgatggatg
    18421 ccggacgcct tcgtgctcgc catcgccttg accgccatcg tcgcgggcgc cgccgctatc
    18481 tttgccaccg acggttcgat cacaacgatc ctaaccggct ggtatggcgg cattttcaac
    18541 atcctcggct tcgcattcca gatgatcctg atcctggttt cgggccatgc gctcgcctat
    18601 tcgccccccg ttcagcgcgg gctcaagcgg ctcgtgctga acatcgccac gcccaatcag
    18661 gcggtcgtgg ttacgttcct gatcgccgcg ctggcctcct ggatcaattg gggtttcggc
    18721 ctcgtcatcg gcgcggtgct ggcgcgcgaa gtggccaaac aggtgcgcgt cgatttcgcc
    18781 tggctggtgg ctgccgccta ttccggcttc gtcatctatg acagcggacc gtcaggttcg
    18841 atcccgctgt cgcaagcctc tcccggcaat gcgctgaaca tcgtggaaaa attcactggc
    18901 cacaccctgc cctatacaca gtcgatcttc acgacgctca accttgtgcc gacgctgctc
    18961 gttctgctcg tcatgccgtt cgtgctcatg ctgatgcggc cgcgcgccgg cgaggtgcag
    19021 ccgttcattc cccccgccga agcgccaata gcggaaaaat cgtcagcgcc ctctttcgcg
    19081 cgcacgctcg agcgttcgcc gctggcaagc ctattcctgg tggcgatcgg cgttgcctat
    19141 gtcgcggtcg ccttcaccag cggccgcctg acgctggacg ccaacatggt gatcttcctg
    19201 ttcctgatcg cggggctcgc gctgcacgga tcgccgctga atttttccga tgccgtcaag
    19261 aacgccgcca aacagaccgg atccatgatc ctgcaatatc cgatctatgg cggcatcatg
    19321 ggtatcatga cggccacggg cctcgccgca cagatctcca gctccattgc ggcatcggca
    19381 tcggcctaca gcctgcccat cctcagcttc ctgtcgtcga tcctgatttc tctggtaatc
    19441 ccgagcggcg gcggacattg ggcggtgcag ggcccgttca ccattccggc ggcggcggca
    19501 ctcaacgcct cggtctcggc caccaccatg gcggtggcga tgggcgaaca ggtcgccaac
    19561 atgctgcaac cgttctgggc gctgccggtg gtcgccatcg ccggcatcgg catccagcgc
    19621 gtgctcgggt tcaccatcgt caccttcatg gtcgcgggct tgatctatgg ggccgcgctg
    19681 ctgatcctga tctgatccgc gcgaggcgat tggctccctt cactgttgcc ggaaaccagc
    19741 gtaactttgc gaaaataatt tgcggggaga cacagatgtc cgattcaaaa ttcacgcggc
    19801 ggcaggtgaa tcgtggatta gcggccggct tcgccatctt ggcatcgcgg ggcgcgcgag
    19861 ccgccgaaat caatttgcaa cacgcgcaca atctccccgt ggaaagcccc ctgcacaagc
    19921 gcgcaacgca attgtggacc gcggtcagga acgaaaccgg cggacgcgtg gacgtccaga
    19981 tcgtccgcga cgattccggt ctggacaggc tggtgcaggg cggcatcgcg ttcatcaccc
    20041 tggcaggcaa caacgtctcc tctctggtgc ccgcagcgga tgtacaagcg acgccttacg
    20101 gcttccgcaa tcccgcccag gtttacgcgg cgctcgacgg tgaactcggc gcgtatttgc
    20161 gtgacgaatt gcgcgccaaa ggcatttacg cgatccccaa cggatgcttc gagaacggca
    20221 tgcaccagat cacgtccaac agccgtccca tccgtacggc ggcggacttc cggggcttga
    20281 aaatccgcat tcccggctcg ccggtctatc aggatttctt caaatcgatg ggggcggaga
    20341 tcgtcacgct gaatctcaca cgcctctatg acgggctgaa agccgggatg gcggaggccc
    20401 aggacgatcc ctgggacgtg atcgaattgt tcaagctcta cgaattccag aaatacgcca
    20461 gcgcgacgga acattcctgg tccggctaca atcttctggc gagcgcgaaa gtctggcagg
    20521 gtttgcctgc cgacgttcag cgggtaatcg aaatcaacac gaaaaaattc gtggcgctac
    20581 agcgcgcgga caatgacgcc ctgaacaacg cgctgcgggc cgacctcacc aagcgcggca
    20641 tgatgttcaa cgaggttgac aaagcctcgt tccgcccgac gctgacggcg ttctatccga
    20701 aatggaaaca gcacatcgga acgcgcgcct gggatttgct tgaagcccat gtcggtcatc
    20761 tcggataaat gagacagcat catgggtcgg attatcgccg tcagccgaag cgccgcgcac
    20821 agtttcagca agccgaacga acccatgatt cagcttgtcg cgggattggg tgtcgcggac
    20881 gatgcccatc tgggcgaaac ggtgcagcat ctggcacgcg tgagggaaga ccccaccaaa
    20941 cccaatctgc gccaggtcca tctgatccat tgcgaactgt tcgacgaatt gcgcgaagcg
    21001 ggtttcagcg ttgcaccggg cgagatcggc gaaaacgtca cgacgcgcgg catcgacctt
    21061 ctgggtctgc ctgcgggcac acggctgcat ctgggcgaaa gcgctgtggt gcaggtcacg
    21121 ggcttacgca acccttgccg ccagctcgac cgctttcagc ccggcctgat ggcggcgatg
    21181 ctgggacgcg acggacaagg acgcattacg ctcaagagtg gaatcatggg catcgtcctt
    21241 gccggcggcc atgtgcgggc gggcgattcc atcgcagtcg cgttgccacc cgagccccac
    21301 cggcctatga cgcgcgtctg aagccgcaga acgcccggga gtgaaccgca tcatgccgac
    21361 cgaaccggcg ccgccggcaa acaatgtcgc cgtggaactg gtgcgcgcct tgcgtgagct
    21421 cgacgtcaaa tatgtattcg gcgtgccgag cggcggctgg gtcgattata tggaagcact
    21481 gcggcaggcg gacggcatcg agttcgtgct gacgacccat gaaggcgggg ccgggatgat
    21541 ggccgaagtc gccggacgtc tcggcccagc cccaggggta tgcttcggca ccttcggtcc
    21601 gggtgcgacg aatctcgcca ccggcgtagg cgaagccttg ctggaccgtt cgccgatgat
    21661 cgcgttcacc gacgagatgc cgccggcgat gcgcggccgc acgacgcaaa tgggaatcga
    21721 ccatcaggcg ttgttccgcc ccctgaccaa gaagaccacg cgtcttgaac catcgcgcgt
    21781 tcgcacgatc ctgttcgatg cggcgcgtat cgcattgcaa gggcgtcctg gtccggtcca
    21841 tgtcgggctg ccggccggca tgtcggccga gcttgtggtg ccggagaaca tcgcattcgc
    21901 tccaccggaa actccgccgc atgcggattc ggcgctgctc gaacaggcgg cggcgctgtt
    21961 tgccgcatcg aaaaaaccga ttctcgccgt cgggcttggc gcggtgcgcg cgcatgcggc
    22021 tgcgaaaatc cgcagcttgg ccgagaggca caaactgccg gtgatcctga cgccgatggc
    22081 caaggggatg cttgcggaag acaatccctg ttatgccggc gtgctgttcc atgcgctgag
    22141 cgacatggtg ggccagacgc acgcgcaagc cgatctggtg atcggcatcg gctatgatcc
    22201 cgtcgagttc aattatgaat cctggctgcc gaaaacagcg cccctcgtca gctttgatat
    22261 tgcaccggcg gatatcgaca cgaacaacca catcctcgcg gtcgatgcgg tgggcgatat
    22321 cgattattcg ctggacaccc tcttggcgct gccgccttcg gaaaaacatt gggacctcga
    22381 cgctttggca aaaaggcgga ccgcgatgtt cgccaagctt gctccttccc ccaagccttc
    22441 ccaaggtgcc gcgctgggac cgaaggcggc actcggtatc ctgcgtgaaa ttctaccggc
    22501 ggacggcatc atgacctgcg atgtcggcgc ccacacccat ctgatcgggc agttgtggca
    22561 tacgcccgcg ccgggcctgc agatcatgac caatggctgg tccaccatgg gtttcggcat
    22621 tccggccgcc atcgccgcca agctgatgcg ccccgcacag agcgtctgtg ccgtggtggg
    22681 cgatggcggc ttcctgatga cggcgggcga actcgcaacc gctatgcgcc tcaatctgaa
    22741 aatcatggtg ctcgtgctga ccgacaatga tctggcactc atccgcatca agcaggagaa
    22801 aaaggccaac ccgatttatg gcacgccggt acgcaaaacg ggcaccatcg gcggcgataa
    22861 tattttcggc gtaccggttc gcaccgccca ggatgccgcg ggtcttcgcg atgcgctgca
    22921 atgggcgttt gccgcctcgg gtccagccat cgtggaagcc attgtcgaca gccgcgaata
    22981 tgacggcctc gtcctcaaaa aagaccggca ataaaatcag gcgatgcgat tcgcgccttt
    23041 gatctgtgta cggtgcatga tgcgccgcgc attctcgtcg aaggggtcgc gccggtgcag
    23101 cacggcgcga ttgttccaca tcaagagatc gccggcccgc cattgatggc acatggtgaa
    23161 acatgtctg
//
LOCUS       DASYEG010000304         2299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1859398, whole genome shotgun sequence.
ACCESSION   DASYEG010000304 DASYEG010000000
VERSION     DASYEG010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1859398"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VGM72_13110"
     CDS             complement(<1..431)
                     /locus_tag="VGM72_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266253.1"
                     /translation="MRRAWVLGLVLVAIAFGGSPNDEWPAYGRDPGGQRFSPLTAINR
                     ENVGLLKVAWTYRTGDGYQPKNGRATSFEATPLYIDGTLYLSTPLGHVLALNPVSGKV
                     RWSYDAKVPRDEGYGDFANRGVSSWKPAHGKRRIIAVTVDAR"
     gene            complement(734..1141)
                     /locus_tag="VGM72_13115"
     CDS             complement(734..1141)
                     /locus_tag="VGM72_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266254.1"
                     /translation="MPAGLETQTLGLQVAVLFLLAVPVACIAWTITHEEIFREPREFC
                     VDRSRRCRNLLQRKFFYALTCEFCLSHYVSAAWLAITRFQLLFDGWRGYLIAWFSLVW
                     IANVYMAIFGRLRLEIQSQRLDIEEEKRQLGKK"
     gene            1287..>2299
                     /locus_tag="VGM72_13120"
     CDS             1287..>2299
                     /locus_tag="VGM72_13120"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY4266255.1"
                     /translation="MRAATELGLRTVAVYSKEDRLGLHRFKADEAYLIGEHLGPVQAY
                     LDVEGIVALAKDKGVDMIHPGYGFLSENPALPRACEQAGITFVGPSAELLELLGDKTA
                     ARKLAQRAGVPTVPGSDNPQDATKVGFPLIVKASFGGGGRGMRVVESEDQFAGKLEEA
                     TREAAAAFGNGAVFLERYIRRAKHVEVQIMGDRHGNIVHLYERDCSVQRRHQKVVEVA
                     PAVNLPKKVRDGLAEAALKLAREAGYYNAGTVEFLVDADSGEFFFIEVNPRIQVEHTV
                     TEMVTGIDLVRCQILVAQGQKLHDQPIGLPKQKDIPLYGYALQCRVTTEDPTNNFTPD
                     YGRL"
BASE COUNT          519 a          736 c          629 g          415 t
ORIGIN      
        1 cgcgcatcca ccgtcaccgc gataatccgt cgcttcccat gagccggctt ccacgacgaa
       61 acgccgcgat tggcaaaatc cccatacccc tcatcgcgag gcactttcgc atcgtatgac
      121 cagcgaacct tgccgctcac cgggttcagc gcgagaacat ggccgagcgg cgtcgaaaga
      181 taaagcgtgc catcaatgta gagcggcgta gcctcaaacg aagtcgcgcg gccattcttt
      241 ggctgataac cgtcgcccgt ccgataagtc cacgcaacct tcaataaccc gacgttctcc
      301 cgattgatcg ccgtcagcgg cgagaaccgc tggccgccgg gatcgcgccc atacgccggc
      361 cactcatcgt tcggcgagcc gccgaacgca atcgcaacca acaccaatcc cagaacccat
      421 gcccgcctca tatctcctga ttatcgccga atgaaagatt ccacacatgg ccatcgatat
      481 gctgccaaat tcagcgaggt gtacgttcat ggcgaatgaa acaaagaaat gcaagcaccc
      541 cgcctgtagg tgcaaggcgc gtgaagacga cgactattgc agcgagttct gcaagggagc
      601 cggtgacatg acttcactct catgcgagtg cggccaccct gcatgtgcac ccctataaaa
      661 ccccattgaa caggtaacgg agaacgattc actgcgcccg caccacgcca cccgagagcg
      721 tgggtgggcg cttttatttc ttgcccaact gccgcttttc ttcctcgatg tcgagacgct
      781 ggctttgaat ctcgagcctc agccggccga aaatcgccat gtagacattg gcgatccaca
      841 ccagcgaaaa ccaggcgatc agatagccgc gccacccgtc gaacaataac tggaaccgcg
      901 tgatagcgag ccacgccgcg ctgacgtagt gacttagaca gaattcgcat gtcagcgcgt
      961 agaagaattt gcgctgtaaa agattccggc agcggcggct tcggtccacg cagaattccc
     1021 ggggctccct gaaaatctct tcgtgagtaa tcgtccacgc gatgcaggca acaggtaccg
     1081 ccaaaaggaa aaggactgcc acctgcaggc caagtgtttg tgtttcgagg ccagcgggca
     1141 ttgcgctgcc acgtgcaatc ctctgaccag gctgaaatcg cggactgaat tggctacacc
     1201 attcagcccc aaacgcacat gccaaaatct tttaagggat gaagaaacta ttagctctca
     1261 accggggcga aatcgcaatc cgcatcatgc gcgccgccac ggagctgggg ctgcgaaccg
     1321 tggccgtcta ttcgaaagaa gaccgcctcg gcctccatcg gtttaaagcc gacgaagcgt
     1381 acctgatcgg cgagcacctc gggcccgtcc aggcttatct ggacgtcgaa ggaatcgtcg
     1441 cgctggccaa agacaaggga gtggatatga tccaccccgg ctatggattc ctttccgaaa
     1501 atccggcgct gcctcgcgcc tgcgagcagg cgggcatcac gtttgtcggc cccagcgccg
     1561 agttactcga attgctcggc gataagaccg ccgcccgcaa actcgcgcag cgcgccggtg
     1621 tccccaccgt ccccggcagc gacaatccgc aggatgccac gaaggtcggt ttccccctca
     1681 ttgtcaaagc ttccttcgga ggcggcggac gcggcatgcg cgtcgtcgaa tcggaggatc
     1741 agttcgcggg caaactcgaa gaagctacgc gcgaggccgc agccgcgttc ggtaatggcg
     1801 ctgtctttct ggagcgctac atccgccggg cgaagcacgt ggaagtgcag atcatgggcg
     1861 atcgccacgg caacatcgtt cacctctatg agcgcgattg ctctgtgcag cgccgtcatc
     1921 agaaggtggt cgaagtcgcg cccgccgtca atctgccgaa gaaagttcgc gacggtctgg
     1981 ccgaagcggc gctgaaactc gcgcgtgagg ccggttatta caacgcgggc acggtcgaat
     2041 tcctggtcga cgccgactcc ggcgaattct tcttcatcga ggtcaatccg cgcattcagg
     2101 tggagcacac cgttaccgaa atggtgaccg gtatcgatct ggtgcgctgc cagattctgg
     2161 tcgcccaggg acaaaagctc cacgaccagc ccatcggcct tcccaagcag aaggatattc
     2221 cgctctacgg ctacgcactg cagtgccgcg tcaccaccga ggaccccacc aacaacttca
     2281 cgccggacta cggccgtct
//
LOCUS       DASYEG010000305         7379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_700656, whole genome shotgun sequence.
ACCESSION   DASYEG010000305 DASYEG010000000
VERSION     DASYEG010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 7379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7379
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700656"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="VGM72_13125"
     CDS             complement(<1..1242)
                     /locus_tag="VGM72_13125"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:TIGR00508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEY4266256.1"
                     /translation="MARSNSPIWHPFTQHGLAGPAIEIARAQGAYLYTSDGRAILDAI
                     SSWWVSTHGHGHPHIASAISDQAAKLEQVIFAGFTHEPAETLTKKLLAIAPKGLAYAF
                     FSDSGSTAVEVAIKMAVGLWHNRGTPRHRILAMEHGYHGDMFGAMAAGHRGIFNAPYE
                     PMLFDVAHLPFPLPGAEHRTLEALQVHLRADAGAIAALILEPLVLGAGGMKMYAPEIL
                     AEIAACCRQYGILLIADEVMTGFGRTGTMFACDQAGVVPDLMCLSKGLTGGFLPMGVT
                     LASAEIYDGFYSSDRNKTFFHSSSFTGNAIACAAGVANLEIWEREPVQPRIDAIAKHH
                     AARLESFRGRSEVAHIRQTGTIAAIALKVPDAGYLAALGPRLNAFYLERDVLLRTLGN
                     VVYVLPPYCVRASELDRIYDVI"
     gene            complement(1232..1876)
                     /gene="bioD"
                     /locus_tag="VGM72_13130"
     CDS             complement(1232..1876)
                     /gene="bioD"
                     /locus_tag="VGM72_13130"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEY4266257.1"
                     /translation="MKPLVVTGTDTDIGKTVVCAMLMLALDAFYWKPLQSGAKDGTDR
                     QRIMTLTGLTAERCFAEGYVLSQPLSPHRAAELDGIQIDPAHLHLPQGPENRWLLIEG
                     AGGVLVPITRDVLQTALFSRWRAPTIVVARTALGTINHTLLTLEALKSRAVDVLGVIF
                     VGDAMPDTEGTIAEMGAVKRLGRLPRLPLLDARSLREAFAAHFSRSDFEEAYGT"
     gene            complement(1873..3003)
                     /locus_tag="VGM72_13135"
     CDS             complement(1873..3003)
                     /locus_tag="VGM72_13135"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEY4266258.1"
                     /translation="MSALLTNIAAEMERLQQAHRVRELSLGHGVDFTSNDYLGLARHP
                     ALREAAIAALEADAILGAGGSRLLRGHHQAHAHLEDFAARFFGVEKTLFMGNGFLANY
                     AIFTTLCGRHDAVVFDERVHASVKEGIHASPAPRFRARHNDLDSFEAAMHRAREKGAR
                     RVFIACESVYSMDGDLAPLKELDALASRYEAVLLVDEAHATGVFGAGGRGLGEERQGE
                     SWIVLHTGGKALGVSGALICARGAVIDYLINKARPFIYSTAPPPHLAAALRRALQLLD
                     EEPWRRTRVLERASFARETLGVPKSPHRPASQIIPIVLGEEARTLDVARALQDAGFDV
                     RAIRPPTVPEGTSRLRISIHADHSEDDIRALAAALRIAAATP"
     gene            complement(3000..3818)
                     /locus_tag="VGM72_13140"
     CDS             complement(3000..3818)
                     /locus_tag="VGM72_13140"
                     /inference="COORDINATES: protein motif:HMM:NF014074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266259.1"
                     /translation="MTIGNLFWVVFIFLALQPVIASRWLKITRSAKIASIQKSRRSRV
                     IAIIHRQEAMRFFGFPIARYIDMDDAEKVLNAIRTTPREQPIDMILHTPGGLALAALQ
                     IARALRAHPGRVTVFVPHFAMSGGTLLTLGANEIVMSEHAMLGPIDPQINGMPAASII
                     KVASEKPLSEVDDQTLIYADIGQKAIDQLKRQACELMRMPITPEAAAALAEKLTSGRW
                     THDYPITAPEARALGLPVNTQMPPEILELMELFPQPVRISQSVEFGPRSDSGSR"
     gene            3954..4040
                     /locus_tag="VGM72_13145"
     tRNA            3954..4040
                     /locus_tag="VGM72_13145"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3988..3990,aa:Leu,seq:caa)
     gene            4250..4519
                     /locus_tag="VGM72_13150"
     CDS             4250..4519
                     /locus_tag="VGM72_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266260.1"
                     /translation="MSSPTEERRDQARKKAQNHFAASEQRDALVRKEIEKERVTSAAK
                     TAKLRALRLAKEASDKEAADKLQGQKAEAKTRKARAPKPKRAPGY"
     gene            4732..4935
                     /locus_tag="VGM72_13155"
     CDS             4732..4935
                     /locus_tag="VGM72_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY4266261.1"
                     /translation="MTIGTVKFFNATKGFGFIAPEGGGKDVFVHATAVEAAGMRSLVE
                     GQRVSFDIQPDARGAKATNLKSA"
     gene            5128..6567
                     /locus_tag="VGM72_13160"
     CDS             5128..6567
                     /locus_tag="VGM72_13160"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4266262.1"
                     /translation="MDSPAPDLAPAFRPTMIALIVAAAFFMETLDGTVIATALPDMAK
                     SFGVGAIQLSIGITAYMLTLAIFLPASGWLADRFGTRNIFGGAITAFTLASIACGLAP
                     DFSSFIAARIAQGAAAALMSPVGRLVVLRATEKRDLLRAAAITIWPGLLAPVIGPPLG
                     GFISTYASWRGIFLLNVPLGLIGVLMVLRYVPQFRDEKRRPFDFIGFSLSGLALGTLM
                     YGFDRIGVREWWSAAALVAAAIVLGWKAVRHLERHDHPLVELSAMRVHSFSVGVWTGI
                     LFRMAVGATPVLLPLLFQRGFGMTAFAAGVLTLGYAIGNIAMKPLTTQILRRLGFRVT
                     LAADALLSSLCIVFCGFLSASTPEWLTTLLLILTGGFRSLGLTGLFTLPFVDVSPSQR
                     TAANTLSNITWQVGMGLGVAFGSIALQASLILRGAGEDSLAVSDFRFAFVVVGVLGAL
                     ALPGFLSLSPDVGEEVSGHRARVSAQAKG"
     gene            6603..7055
                     /locus_tag="VGM72_13165"
     CDS             6603..7055
                     /locus_tag="VGM72_13165"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY4266263.1"
                     /translation="MRRWFKSTSKRTFIVYPIGVMAFESGLHRGLAFHAWGVVFLLWG
                     YLQYRLVGFYRVRLGGGGPGLDVPPDHIVARGPYRFLRNPMYLGHLIFMLGLTVTFSS
                     WLAAALLAFHIVWFHKRVLADEVRLETRFGAPYLAYKSHVKRWIPGFF"
     gene            complement(7088..>7379)
                     /locus_tag="VGM72_13170"
     CDS             complement(7088..>7379)
                     /locus_tag="VGM72_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEY4266264.1"
                     /translation="ATVARALYILSLGRMVITDRLHGHLFCLLLGIPHVLLNNDSGKN
                     WNFYETWTRESPLCRLARNPAEAWSLARNAMPKLRETAGDPWSWQGVETQNP"
BASE COUNT         1323 a         2191 c         2378 g         1487 t
ORIGIN      
        1 gatgacatca taaatcctgt cgagctcact tgctcgcacg caatagggag gaagaacgta
       61 gacgacattc cccagggtgc gaaggagcac gtcgcgctca agatagaacg cgttcagccg
      121 cgggccaagc gcggccaaat aacccgcatc ggggaccttg agcgcgattg cggcgatggt
      181 tcctgtctgg cggatgtggg cgacctcaga tcgcccgcga aaagactcaa gccgcgcggc
      241 gtggtgcttg gcaatcgcgt cgatgcgtgg ctgtaccggc tcgcgttccc agatttccag
      301 attggcgaca cctgcggcgc aggcgatcgc gttgccggta aaggacgagc tgtgaaagaa
      361 ggtcttgttg cggtcgctgg aatagaaacc gtcgtaaatc tccgcgctcg cgagcgtcac
      421 gcccatgggc aggaagcccc cggtgagacc tttggagagg cacatcagat cggggaccac
      481 gcccgcctgg tcgcaggcga acatcgttcc ggtgcggccg aaaccggtca tgacttcgtc
      541 ggcgatgagg aggatgccgt attggcggca gcacgcggcg atttcggcca atatttcggg
      601 cgcgtacatt ttcatcccgc cggcgccgag caccaggggc tccagaatga gcgccgctat
      661 ggcaccggca tcggcgcgca aatgtacttg caaagcctcc aaggtgcgat gctcggcccc
      721 tggcaagggg aacggcagat gcgcgacatc aaacagcatg ggctcatagg gcgcattgaa
      781 gattccgcga tgtccggccg ccatcgcgcc gaacatgtcg ccatgatagc cgtgctccat
      841 ggcgaggatg cgatggcgcg gcgttccacg attatgccag aggccgaccg ccatcttgat
      901 cgccacttcc acggcggtcg aaccgctgtc ggaaaaaaat gcgtaggcga gccccttggg
      961 tgcgatcgcg agaagctttt tcgtcaacgt ctcggccggc tcatgcgtga agcccgcgaa
     1021 gatcacctgt tccagtttcg ccgcctggtc cgaaatggcg cttgcgatgt ggggatgacc
     1081 gtggccatgc gtgctcaccc accatgagga gatcgcgtcg aggatcgcac ggccgtcgga
     1141 agtgtagaga taggcgcctt gcgcgcgcgc aatttcaatc gcgggtcccg ccagtccgtg
     1201 ctgggtgaag ggatgccaga tgggcgaatt gctacgtgcc ataagcctcc tcgaaatcgc
     1261 tccggctgaa gtgggcggcg aatgcctcgc gcaaagatct ggcgtcgagc aacggcagac
     1321 ggggcaagcg tccgaggcgc ttcaccgcac ccatttccgc gatggtgcct tcggtatccg
     1381 gcatggcatc gccgacgaag atcacaccca ggacgtcaac ggcgcggctt ttcaacgctt
     1441 ccagggtcag aagcgtgtgg ttgatcgtgc ccagcgcggt gcgcgccacc acgatcgtag
     1501 gtgcgcgcca gcgtgagaaa agtgctgtct gcaaaacatc acgcgtgatc gggaccagca
     1561 cgccgccggc accttcgatc aacaaccagc gattctccgg accctgaggc aaatgcaaat
     1621 gggcgggatc gatctgaatg ccgtccaatt cggcggcgcg atgcggcgac aatggctggc
     1681 tgagaacgta accttccgcg aagcagcgtt cggccgtcag gccggtcagg gtcatgatgc
     1741 gctgacggtc cgtcccgtcc ttggctccgg actggagcgg tttccagtag aacgcgtcga
     1801 gcgcgagcat cagcatcgcg cacaccacgg tttttccaat atcggtgtcc gtccccgtca
     1861 cgacgagtgg tttcatggcg ttgccgcggc gatgcgcaag gctgcggcca gtgcgcgaat
     1921 atcgtcttcg ctatggtcgg catggatgga gatgcgcagg cgcgaggttc cctccggaac
     1981 ggtgggaggg cgtatcgcgc ggacgtcgaa gcccgcatcc tgcagcgcgc gcgcgacgtc
     2041 cagcgtgcgt gcttcttcgc cgaggacgat gggaatgatc tggctggcgg gccggtgagg
     2101 ggacttcggc actccgagcg tctcgcgcgc gaatgacgcg cgttcgagca cgcgcgtccg
     2161 gcgccagggt tcttcgtcca acaattgcag tgcgcgccgc aaagccgccg cgaggtgcgg
     2221 cggcggcgcc gtgctgtaga tgaaggggcg cgccttgttg atgagataat cgatgaccgc
     2281 gcccctggca cagatcaggg cgccggatac gcccaaggct tttccccccg tgtgcagcac
     2341 gatccaagac tcgccctgcc gttcttctcc cagccctcgg ccgcctgcgc cgaaaacccc
     2401 tgtcgcgtgg gcttcgtcga cgagaagcac ggcctcatag cggctggcga gcgcatcgag
     2461 ttctttcaga ggcgcaagat cgccgtccat gctgtagacc gattcgcagg caatgaagac
     2521 gcgccgcgcc cctttctccc gcgcgcgatg cattgcggcc tcgaaggaat caagatcgtt
     2581 gtgccgtgcg cggaatcgcg gcgcggggga ggcatggatg ccttccttga cgctggcatg
     2641 cacgcgctca tcgaaaacca ccgcgtcatg gcgcccgcaa agcgtggtga agatcgcgta
     2701 attggcgagg aacccatttc ccatgaaaag cgtcttctcg acgccgaaga agcgggccgc
     2761 gaagtcctcc agatgagcat gggcctgatg atgcccgcgc agaagccgcg agccgcccgc
     2821 gccgaggatg gcatctgcct ccagagccgc gatcgctgct tcgcgcagtg ccggatgacg
     2881 cgcgagcccc agataatcgt tcgacgtaaa atccacgcca tggccaagcg aaagttcgcg
     2941 tacccgatgc gcctgctgca gccgctccat ttcggcggca atattggtaa gcagcgcgct
     3001 caacgcgaac ccgaatccga gcgcgggccg aattcgacgc tctggctgat gcgcaccggt
     3061 tgcggaaaca actccatcag ttcgagtatc tccggcggca tttgggtatt gacgggcaag
     3121 cccagcgcgc gcgcttcggg agcggtgatc gggtaatcat gggtccagcg gccacttgtg
     3181 agtttttcgg ccagtgccgc ggccgcttcg ggcgttatcg gcatccgcat cagctcgcaa
     3241 gcctggcgtt tcaattggtc gatcgccttc tggccgatat cggcgtagat cagcgtctgg
     3301 tcatcgactt cggagagcgg tttttcgctc gccactttga tgatggatgc cgccggcatc
     3361 ccattgatct gcggatcgat ggggcccagc atcgcatgct cgctcatcac aatctcattg
     3421 gcccccagcg tcagcagcgt gccaccggac atcgcgaagt gcggcacgaa cacagtgacc
     3481 cggccgggat gggcgcgaag ggcgcgcgcg atctgcagcg cggcaagcgc aagcccgccg
     3541 ggcgtgtgca ggatcatgtc gatgggctgt tcgcgcggcg tggtgcgaat ggcgttgagc
     3601 actttttccg catcgtccat atcgatatag cgcgcgatcg gaaagccgaa aaaacgcatc
     3661 gcctcctggc ggtggatgat cgcgatgacg cgcgaccggc gcgatttctg aatgctggcg
     3721 atcttggccg agcgcgtgat cttcagccag cgcgacgcga tgaccggctg tagcgccagg
     3781 aatataaaca caacccagaa cagattgccg atcgtcatca ccgacctcgg gagaccgcgt
     3841 gagactatca tagggaggat tgcgccggtt tgtccgccag acttgcgaga ctttgttgac
     3901 gagggggact tcgtggctat gatccgcccc ttccggttcg attttcctga cttgcccccg
     3961 tggcggaatc ggtagacgcg gcagactcaa aatctgttgc cttcacgggc gtgctggttc
     4021 aagtccggcc gggggcacca gccgtcgctg tttcggagct ttgactggcg gctggcggta
     4081 ctctaaggat gaatggtgcc cttgaataag gcgtcccatg gcttcatcgt aggcctgagg
     4141 cgagggcgct ggcgttgggg ggagggcgcg ccgccggttt tattcttctc taaccggctt
     4201 ccgggagcgc gcaatggcgc atcggggatg ccttacagaa agaattgcca tgtccagtcc
     4261 cacggaagaa cgccgggatc aggcccgcaa gaaggcgcaa aaccatttcg cggcgtccga
     4321 gcagcgcgat gccctggtgc gcaaggaaat cgagaaggaa cgcgttacaa gtgccgcgaa
     4381 aacggcaaaa ctgcgcgctt tgcgccttgc caaggaggct tccgacaaag aggccgccga
     4441 caagctccag gggcaaaagg cggaggccaa gacgcgaaag gcccgcgcac ccaagcccaa
     4501 gcgggcacca gggtattagc tctcgacata ttaccgctta ttgcaaaatg gccctgttag
     4561 cgataatttg ccggtgcgat ggcgtcccgt cagatgaagc gcacctgttg aatactggac
     4621 tcgcttaagt gcgcgcctgg ccgagacagc cgatcctttc aactgaacgt acgcacaatt
     4681 cgcactgcat ggcatgtcgc cattgtagaa accgggcata ggaaacgttc aatgaccatc
     4741 ggaaccgtaa agttcttcaa cgccaccaag ggttttggat tcatcgcgcc tgaaggcggc
     4801 ggcaaggacg tgtttgtcca tgcgacagcc gtcgaagcgg cgggaatgcg cagccttgtc
     4861 gagggccagc gtgtctcctt cgacattcag cccgatgcgc gtggtgcgaa ggcgaccaat
     4921 ctgaagtccg cttgatcggc ttcaggatgt gatcggctgt ggcgaaagat tcgccagccg
     4981 atcggtgggt acatacaagg gcgccgggaa gaaattcacg gcgcccttgg cttgccagga
     5041 gagacggccc gtgccgctat cccggtgccg ctgtccgatg atccgatttt tttgaatcgc
     5101 cgtaaaaccg ttcgatagag acagtcgttg gattcccctg ctccagacct cgcgccggcg
     5161 ttccggccga cgatgatcgc cctgatcgtt gccgctgcgt ttttcatgga gacgctcgac
     5221 ggcacggtga tcgcgaccgc cctgccggac atggcgaaat ccttcggcgt gggcgcgatc
     5281 cagctcagca tcggcatcac cgcctatatg ttgacgcttg cgatctttct tccggcgagc
     5341 gggtggctgg cagaccgttt tgggacgcgt aacattttcg gcggcgcgat tacggcattc
     5401 acgctcgcgt ccatcgcctg cgggctggct ccggattttt ccagcttcat cgcggcgcgc
     5461 atcgcccagg gcgcggcggc tgcgctgatg tcgcccgtcg gacggctggt ggtgctgcgc
     5521 gcgaccgaga agcgcgatct tttgcgggcg gccgcgatca ccatctggcc cggactgctg
     5581 gcgcccgtca tcggcccgcc cttgggcggc tttatttcga cctacgcgag ctggcggggt
     5641 atattcctgc tgaatgttcc cctcgggttg atcggcgtgc tgatggtgtt gcggtacgtg
     5701 ccgcaattcc gggacgaaaa gagacgtccg ttcgatttca tcgggttttc cctgtcggga
     5761 ctggcactcg ggacactgat gtacggtttc gaccgaatcg gtgttcgcga gtggtggtcc
     5821 gcggccgcgc tggtggcggc ggcgattgtg ctgggatgga aagcggttcg gcatttggag
     5881 cgtcatgacc atcctttggt ggaactgtcg gccatgcgcg tgcactcgtt ttcggtcggg
     5941 gtatggacgg gcatcctgtt ccggatggcg gttggcgcga cgccggtcct gctgccgttg
     6001 ctgtttcagc gcggctttgg catgacggcc tttgccgcag gcgtgctgac gctgggttat
     6061 gcgatcggca atatcgccat gaagccgctc accactcaga tattgcggcg gcttggcttt
     6121 cgcgtgacgc ttgccgccga cgcattgctg tccagcctct gcattgtctt ttgcggattt
     6181 ctttcagcgt cgacaccgga atggctgacg acgctgctgc tcatcctgac cggcggtttc
     6241 cgctcgctgg gactgaccgg cttgttcacg cttcccttcg tcgatgtctc gccgagccag
     6301 cgcacggccg cgaacacgct ttccaacatc acttggcagg tcgggatggg actgggcgtc
     6361 gcttttggaa gcatcgcgtt acaggcttcg ctgatcttgc gcggagcagg cgaagattcg
     6421 ctggctgttt cagattttcg cttcgccttt gtcgttgtgg gggttttggg tgcgctggcc
     6481 cttccaggct ttctcagcct gtcgcctgat gtcggcgaag aggtcagcgg ccatcgtgcc
     6541 cgcgtttcgg cacaagcgaa aggttgagcg ggttggagcg caatgcatcc ccctggcgag
     6601 gattgaggcg ctggttcaag agcacctcga agcggacctt catcgtctat cctatcggag
     6661 tgatggcatt tgaatcgggg ctgcatcgtg ggctggcgtt tcacgcttgg ggcgtggttt
     6721 ttttgctttg gggttacctg caataccgac tggtgggctt ttatcgggtg aggctgggcg
     6781 ggggcgggcc gggtctggac gttccacccg accatattgt ggcgcgcggc ccttatcgct
     6841 tcctccgcaa cccgatgtat ctcgggcatc tgatcttcat gctgggtttg acggtgacat
     6901 tttcgtcatg gctggcggcc gcactgctcg cctttcacat agtgtggttc cacaagcggg
     6961 tgctcgccga cgaggtccgc ctggaaaccc gtttcggcgc gccctacctc gcgtacaaga
     7021 gccatgtgaa acgctggatt ccaggatttt tttgaagctt cgcccctcct gtcagcgtgt
     7081 tccggcatta cgggttttgc gtctccacgc cctgccacga ccatgggtcg cctgccgttt
     7141 ccctcagctt gggcatcgcg ttgcgcgcga gcgaccaggc ttccgcgggg tttcgcgcca
     7201 gccggcatag tggcgactcg cgcgtccagg tctcgtagaa attccagttc ttgccggaat
     7261 cattgttcag gagaacatgc ggaattccga gcagaagaca gaacaggtgt ccatgcagcc
     7321 ggtcggtgat aaccatacga ccgagcgata gaatatacag tgcgcgagcg accgtggct
//
LOCUS       DASYEG010000306         6627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3311328, whole genome shotgun sequence.
ACCESSION   DASYEG010000306 DASYEG010000000
VERSION     DASYEG010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6627
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3311328"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            292..1455
                     /locus_tag="VGM72_13175"
     CDS             292..1455
                     /locus_tag="VGM72_13175"
                     /EC_number="2.7.7.60"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF006899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase/2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase"
                     /protein_id="HEY4266265.1"
                     /translation="MTQTVALIVAAGRGERARRAGDSTLPKQYSELAGQPVLRWTAGA
                     FLRHAAVSAVQVVIRDEDRASYRSAVAGLTLPPPVCGGATRQESVHRGLESLIPLRPD
                     RVLIHDGARPLVSQTLIARVAQALDEADAVAPLLGVADTLRRKTPQGYAIQPRDDLFR
                     AQTPQGFRFAAILDAHRRFAGEPVTDDFALAERGGLSLASVPGEEMNLKLTTPEDFVL
                     AERLAASRLADVRIGTGFDAHGFGSGDSVWLCGVNVPHDFGLEGHSDADCGLHALTDA
                     ILGAIASADIGAHFPPSEERWRGAGSHLFLAHAASLVRAQGGIISHVDVTLLCERPKI
                     GPHRDAMRARMAEILGMDIARVSVKATTTDGLGFIGRREGIAAQAVATIRLPV"
     gene            1452..1916
                     /locus_tag="VGM72_13180"
     CDS             1452..1916
                     /locus_tag="VGM72_13180"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEY4266266.1"
                     /translation="MTPISTRLATCFGIGWSPLAPGTCASAAALPLGWLLIRLGWPAL
                     VIGAGLASVCGIWACGVHARKVGLYDPSECVLDEVAGQWIALLPIVLLGHGRDWRPYG
                     VAFLLFRLFDIWKPWPISAAEKLPGGFGVMMDDILAGCAAAVLLYAALTWLA"
     gene            1916..2404
                     /locus_tag="VGM72_13185"
     CDS             1916..2404
                     /locus_tag="VGM72_13185"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEY4266267.1"
                     /translation="MFPDSVIDLAKTLLEESRIAGLHIVTAESCTGGSIAAALTEIAG
                     SSDVFDRGFVVYSDRAKTELLGVPAELIRRHGAVSEDVARAMAEGALKHSPAQLAVSV
                     TGIAGPGGATLGKPVGLVHLAAAREGHTTLHEECRFGDIGRSHVRMRSVEEALKLLLQ
                     IL"
     gene            complement(2384..2845)
                     /locus_tag="VGM72_13190"
     CDS             complement(2384..2845)
                     /locus_tag="VGM72_13190"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEY4266268.1"
                     /translation="MPLHQETRILPYTAEQIYAVVADIEHYPQFLPWCSKLIVRKREK
                     RGATELVTAEMVVSYHALHERYTSCVALDPLGRTIEARHIEGPFRKLDTRWRFVPTET
                     GSEVYFLIDFAFKNALLSAVANVGFGYVANRMAQAFVDRAKALYGSEDLKQ"
     gene            complement(2852..3808)
                     /gene="lipA"
                     /locus_tag="VGM72_13195"
     CDS             complement(2852..3808)
                     /gene="lipA"
                     /locus_tag="VGM72_13195"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY4266269.1"
                     /translation="MTVIYDSATKDLRPRHPEKANRPDTALPKKPEWIRVKAPTGRGY
                     AMTRDIVREHGLHTVCEEAGCPNIGECWEKAHATMMIMGDTCTRACAFCNVRTGLPGA
                     LDADEPENVARAVAKLGLSHVVITSVDRDDLDDGGAAHFVRTVRAIRAHSPRTTIEIL
                     TPDFLRKLGAVETVMTAPPDVFNHNLETVPRLYLTVRPGARYFISLRLLARVKEIDAH
                     AFTKSGLMVGLGETREEIMQVMDDLRAANVDFLTIGQYLQPSRKHAAVERFWTPDEFK
                     ALEAIARAKGFLMVSASPLTRSSYHAGEDFARLKAARAAAHP"
     gene            4007..4384
                     /locus_tag="VGM72_13200"
     CDS             4007..4384
                     /locus_tag="VGM72_13200"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4266270.1"
                     /translation="MLAAKDAMATIAVKDIATAKTFYGGILGLKPFGSESDGVILYRS
                     GNSTIVVYQSQYAGTNKATSATWGVGEELEAIVQILKNAGVSFEHYDFPGLRREGDVH
                     IAGDFRAAWFRDPEGNILHINSM"
     gene            4453..6036
                     /locus_tag="VGM72_13205"
     CDS             4453..6036
                     /locus_tag="VGM72_13205"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY4266271.1"
                     /translation="MAGSPIPYNPTKARRTLFRALNDASAAFGPKRAILIDGDDRVLT
                     YGELQLAALALGHALKKGTRKGESVGVLLPTGIGAVIATFALSAYGRVPAMLNFTAGL
                     QSLQAALSAAKIGRILTAHRFIELGRFQSLETELKKCAELIYLDDLRGKLSFWDKAAA
                     AIGSKLPAVIATRTSPDSPAVILFTSGTEGEPKGVVLSHANILANVEQVRAHIPLTAS
                     DIVFNPLPTFHSFGLTVGALMPVCLGIKAVLHPTPRQPREIAQRIRENAATILLATDT
                     FVSQYMRAAGDGDLSSLRFAVCGAERLHDETRQFARRNYDFELLEGYGVTEGAPVLSA
                     NQPGANRPGTVGRLVACVQSRIEPVEGIREGGRLFVKGPNIMLGYLSPGRPGEIVALP
                     DGWHDTGDVVSLDADGYIAIRGRLKRFAKIGGESVSLTVVENCASALWPDHAHAAITM
                     PDGRKGESIVLLTTCPDAGRDELLVWARNHGVPELAVPRRVIPIADVPVLGTGKTDYL
                     AVARIAAGMIEKPTSSVHE"
     gene            6221..>6627
                     /locus_tag="VGM72_13210"
     CDS             6221..>6627
                     /locus_tag="VGM72_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266272.1"
                     /translation="MAGPYVTSEGGSLSAPPSDTRQATLAQTESFGFGNSYGTAYGYN
                     LGHGFKTEIEGTATGAASDRLGGLAAGGSLTGTRVTLKGMYEFSEGAWHMKPYIGAGF
                     GVVDMNQHVLGISGNQWVSAYQLRGGVTLGFTQ"
BASE COUNT         1190 a         2021 c         2083 g         1333 t
ORIGIN      
        1 cagcaaaagg cccacgccca tgtcgaagaa ttgctccgcc gcctgattgg catagagcag
       61 ccggtcgcct gtcccaacca gaaggatggg cattggaagc gcgcctgtaa tgatgccgct
      121 atgcccagta gcggctctgg gttcgcggat ttcgcctaat cttttgttgc cggtcacaat
      181 tttatttgcc tatatcttgg gcagatatag gctatgccta ttcaatgagc aatcgctaaa
      241 aaagcgaaat ccgcggcttt ccacatgaaa gatgggcgga tacagaaagc catgacccag
      301 actgtcgccc ttatcgttgc cgcgggacga ggtgagcgag cccggcgggc cggtgacagc
      361 accctgccca agcaatacag cgaactcgcc ggacagcctg tgttgcgatg gacggccggg
      421 gcgttcctcc gccatgcggc cgtatcggct gtgcaggtcg tgatccggga cgaggaccgt
      481 gcgtcctatc gatcggctgt tgctggccta acgctgcccc ctccggtttg cggcggtgca
      541 acgcgacagg aatccgtcca tcgcggtctc gaatccctta tcccgttgcg gcccgatcgc
      601 gtgcttattc acgatggggc gcggccgttg gtgtcccaaa cgctgatcgc gcgcgtcgca
      661 caggcgcttg acgaggccga tgcggtagcg cccctgttgg gcgtggccga cacgctgcgg
      721 cgaaagacgc cgcagggcta cgccattcag ccccgcgacg atttattccg ggcacaaacg
      781 ccgcagggtt ttcgttttgc ggccattctc gatgcccacc gcagatttgc cggcgagccg
      841 gtcacggatg atttcgcgct cgccgaacgc ggcggactct cgctggcaag cgtgcctgga
      901 gaagagatga acctgaaact gacgaccccc gaagacttcg ttctggccga gcgtctcgcg
      961 gcctcgcgct tggcggacgt gcggattggg accggcttcg atgcccatgg ttttggatcg
     1021 ggcgactccg tctggctttg tggggtgaat gttccccatg atttcggtct ggagggtcat
     1081 tcagacgccg attgcggcct ccatgcgctg acggatgcga ttctcggcgc catcgcttcg
     1141 gccgatatcg gtgcgcattt cccccccagc gaggaacgct ggcgcggcgc gggctcgcat
     1201 ctctttcttg cgcacgccgc atcgttggtt cgcgctcaag gcgggatcat ttcccatgtc
     1261 gatgtgacgt tgctctgcga gcgccccaaa atcggtccgc accgcgatgc catgcgcgcg
     1321 cgcatggccg aaattctcgg catggacatc gcgcgcgtga gcgtaaaagc gacaaccacc
     1381 gacggcctcg gattcatcgg ccgccgcgag ggcatcgcag cccaggcggt ggcaacgatc
     1441 cggctgcctg tatgacgccg atctcgacac gcctcgccac ctgcttcggg atcggatggt
     1501 cgccgcttgc acccggaaca tgcgcttctg ctgcggccct ccccttggga tggctgttga
     1561 tacgcctcgg ctggcctgcc cttgtcatag gcgcgggcct tgccagcgtg tgcggcattt
     1621 gggcctgcgg cgtgcacgcc cgcaaagtgg gtctgtacga tccatccgaa tgcgtgctcg
     1681 acgaggtggc ggggcaatgg atcgcgctgc ttcccatcgt gctgctgggc catggccgcg
     1741 actggcggcc ctatggcgtc gcgtttctcc tgttccgcct gttcgacatc tggaagccgt
     1801 ggcccatttc cgccgctgag aaattgcccg gcggattcgg cgtgatgatg gacgacatat
     1861 tggcgggatg cgccgctgcc gttcttcttt atgcggcgct gacatggttg gcgtaatgtt
     1921 tcccgattcc gtgatcgatt tggcgaaaac tttgctggag gaatcgcgca tcgccggact
     1981 tcatatcgtg accgccgaga gctgcacggg aggatcgatc gcggcggcgc ttaccgagat
     2041 tgccggttcg tccgacgtgt tcgatcgcgg cttcgtcgtt tattccgatc gcgccaagac
     2101 cgaattgctc ggcgtgccag cggagttgat ccgccggcat ggcgcggtat cggaggacgt
     2161 cgcgcgtgcg atggccgaag gggcgctgaa acattcgccc gcgcaattgg ccgtctccgt
     2221 caccgggatc gcggggcccg gcggcgcgac ccttggaaaa cccgtcgggc ttgtccattt
     2281 ggccgccgcg cgcgaaggac atactaccct ccacgaagag tgccgctttg gcgacatcgg
     2341 acgcagccat gtgcgcatgc gcagcgtgga agaggccttg aagctactgc ttcagatcct
     2401 ctgatccata gagcgccttc gcacgatcca cgaacgcttg ggccatgcga ttcgccacat
     2461 agccgaagcc gacattggca acggccgaaa gaagcgcgtt tttgaaggca aaatcgatca
     2521 ggaagtacac ttccgatccg gtctcggtgg gtacgaaacg ccagcgcgtg tcgagtttgc
     2581 ggaacggccc ctcgatatgg cgcgcctcga tggtgcgccc gagaggatcg agagcgacgc
     2641 agctggtata gcgctcgtgc aacgcatgat aggaaacaac catctccgcc gtgaccaatt
     2701 cggtcgcgcc ccgcttctca cgtttgcgga cgatcagctt cgagcaccat ggcaggaatt
     2761 gcggatagtg ctcgatatcg gcgaccacgg cgtatatctg ctccgccgta tacggaagaa
     2821 tgcgcgtttc ctgatgaagc ggcattggtt cttaaggatg cgccgcggcg cgcgccgcct
     2881 tgagccgcgc gaaatcctcg cccgcgtgat aggaggatcg cgtcaaaggg ctcgccgaca
     2941 ccatcaggaa tcccttggcg cgggcgatgg cttccaaggc cttgaattca tccggcgtcc
     3001 agaagcgctc caccgcggca tgcttgcggc tcggttgcag atactggccg atcgtgagga
     3061 agtcgacatt ggccgcgcga agatcgtcca tcacctgcat gatctcttcg cgcgtctcgc
     3121 caagtcctac catcagtccg gacttcgtga aggcatgcgc gtctatctcc ttcacgcggg
     3181 cgagcaatcg gagcgagatg aaataacgcg cgcccggtct caccgtgagg tacagccgcg
     3241 gtacggtttc gagattgtgg ttgaagacgt ccggcggcgc cgtcatcacg gtctccacgg
     3301 cgcccagctt gcgcaggaaa tccggtgtca gaatctcgat cgtggttctt ggcgaatgcg
     3361 cgcggatcgc gcgcacagtc cgcacgaaat gcgccgcgcc gccatcgtcg agatcgtcgc
     3421 ggtcgacgga ggtgattacc acgtgtgaaa ggcccagttt cgcaaccgcg cgcgcgacat
     3481 tttccggctc gtccgcgtcc aatgcacccg gaagccccgt gcgcacattg cagaaggcgc
     3541 aggcccgcgt gcaggtgtcg cccatgatca tcatggtggc gtgcgccttt tcccagcact
     3601 cgccgatgtt gggacaaccc gcctcctcac acacggtgtg cagcccatgt tcgcgcacga
     3661 tatcgcgtgt catggcgtag ccgcgcccgg taggagcctt gacgcggatc cattcgggct
     3721 ttttgggcag cgcggtatcg ggccggttcg ccttttccgg atggcgcggc cttaaatcct
     3781 tcgtcgcgga atcgtagatg accgtcatga tccattccta acgcttgcgg gttaggaaat
     3841 cacccctttc gtcgaggcgc tacgccctga tgccagaatg tccatgggca tgcccgcctt
     3901 ggccacccct gcaaccctaa catacgctcc gcaacgaaat cgcaggcgga cagttattgc
     3961 atccgggacg tcaatcgaac ggccgatgat gaaggggaga attgcgatgc tggctgccaa
     4021 ggatgcgatg gcgaccatcg cggtgaagga catcgcaacg gccaagacat tttatggcgg
     4081 tattttgggg cttaaaccgt tcggttccga aagcgatggc gtgatccttt accggagcgg
     4141 gaattccacc atcgtcgtct atcagtccca atatgccggc acgaacaaag ccacctcggc
     4201 gacctggggt gtgggcgagg aattggaggc catcgtccag atcctgaaaa atgccggtgt
     4261 ttcctttgaa cactacgatt ttccagggct gcggcgcgag ggcgatgtcc atatcgcggg
     4321 agacttcaga gcggcctggt tcagggatcc cgaaggcaat attctgcaca taaacagcat
     4381 gtgagttttc gcggcgcccg cttggtgcgg ccgtccggcc gcgctaggct tctgctaaag
     4441 cgggacacaa gcttggccgg ttctcccatt ccctacaatc cgacaaaggc gcggcggacg
     4501 ttgtttcgcg cgcttaacga tgccagcgcc gcgttcggtc ccaagcgtgc cattttgatc
     4561 gacggcgacg accgcgtgct gacctatggc gagcttcaac tggccgcgct ggcgctcggc
     4621 catgcgttga aaaaaggaac gcgcaaaggc gagtcggtgg gtgtgctgct gccgacggga
     4681 atcggcgcgg tgatcgcgac gttcgcgctt tccgcctatg gccgcgtccc ggccatgctc
     4741 aatttcacgg ccgggctaca aagcctgcag gcagcgttga gcgcggccaa gatcgggcga
     4801 atcctgaccg cccaccggtt catcgaactg ggcaggtttc agagcctcga aaccgaattg
     4861 aaaaaatgtg ccgaactgat ttacctcgac gatctccgcg ggaagctctc gttttgggac
     4921 aaggccgccg ccgccatcgg ttcgaaactc ccggccgtga ttgccacgcg aacctcgccg
     4981 gattcaccgg ccgtgattct gttcacgtca ggcacggaag gcgagcctaa aggcgttgtg
     5041 ctgtcgcatg ccaacatcct cgccaatgtc gagcaggtgc gggcgcacat tccgctgacc
     5101 gccagcgaca tcgtgttcaa tccgctgccg acctttcatt ccttcgggtt gacggtgggc
     5161 gctttgatgc cggtctgtct tggcatcaag gccgtgctgc acccgacccc gcgtcagccg
     5221 cgcgagatcg cgcagcggat tcgcgagaac gccgcgacca tccttttggc gaccgatacg
     5281 ttcgtctcgc aatacatgcg cgcggcgggc gatggcgatt tgtcgagtct caggtttgcc
     5341 gtctgcggcg ccgagcgctt gcacgacgaa acccgccagt tcgccaggcg gaattatgat
     5401 ttcgagttgc ttgagggcta cggcgtgacc gaaggcgctc cggtgctttc ggccaatcag
     5461 ccgggcgcga accggccggg caccgtgggt cgccttgtgg cttgtgtcca atcgcgcatc
     5521 gaaccggtgg aaggcatccg cgaaggcgga cggttgttcg tgaaggggcc caatatcatg
     5581 ctgggctatc tctcgcccgg ccggccgggt gagatcgtag ccctgcccga tggatggcac
     5641 gacaccggtg acgtggtcag tctcgatgcg gacggctaca tcgccattcg cggcaggctc
     5701 aagcgtttcg ccaaaatcgg cggtgaatcg gtctctctca ccgtggtgga aaactgcgca
     5761 tcggccttgt ggcccgatca tgcccatgcg gccatcacca tgccggacgg gcgaaaaggc
     5821 gagagcattg tcctgctgac cacatgtccg gacgcgggac gggacgagct tctggtgtgg
     5881 gcgcgaaatc acggagtgcc ggaacttgcg gtgccgcgcc gcgttatacc gattgccgat
     5941 gttcccgtgc ttggcacggg caagacagac tatttggctg tcgccaggat agccgccggc
     6001 atgatcgaga aacccacatc gtccgtgcac gaataatccc tctccggcgg cggcaatttt
     6061 tgcgattcac gccggtgacc tcaaaaccgg tttgcgcttt gcgcgttttc gtgatcgggc
     6121 gccgcagtcg acggcgcgga tgggcggaac gaagtctatg cggagttgga cgctcgttgg
     6181 gattgggatc gcgcttcttt ccggaagcgc aaatgcccaa atggcgggcc cctatgtaac
     6241 ctccgaaggc ggttcgcttt cggctccccc ctcggacacg cggcaagcca cactggcgca
     6301 aacggaatcg tttggattcg gcaattctta cggcacagcc tatggttata atctcggcca
     6361 tggcttcaag accgagatcg aaggcacagc gaccggcgcc gcgagcgatc gcctgggcgg
     6421 cctcgcggcg ggcggcagtc ttaccgggac gcgtgtgacg ttgaagggca tgtacgaatt
     6481 cagcgaaggt gcctggcata tgaagcccta tatcggagcg ggcttcggcg tggttgacat
     6541 gaaccagcat gtgctcggca tcagcggcaa tcagtgggtt tccgcctatc aattgcgtgg
     6601 cggcgtcacg ctaggcttta cccagaa
//
LOCUS       DASYEG010000307         1915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4901760, whole genome shotgun sequence.
ACCESSION   DASYEG010000307 DASYEG010000000
VERSION     DASYEG010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4901760"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VGM72_13215"
     CDS             complement(<1..873)
                     /locus_tag="VGM72_13215"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266273.1"
                     /translation="MIVGNTFDAAARENAVHWVHSSQDDVAMVRDFVKWDDRPASLQH
                     FAESAVRAYKIATTPPMGPVILACDTEMQEGPIPEGEKPTIPKLPVTAMPQGESGAIA
                     EVAKLLVAAENPVIVAESYGRSQAAVDSLVELAELLQCGVIDVSARMNFPTRHPLNQT
                     SRGGRGTVGQADVVLGLEVVDFWGVTHQFREHIVSSSRATTKPGAKIISISSADLFIK
                     SNFQNFQRFEPVDLAIAADAATTMPVLIEAVKRAMDGGRKAAFAARGQKLGEAHKTAL
                     ERVRTEAAYGWDASP"
     gene            complement(870..1355)
                     /locus_tag="VGM72_13220"
     CDS             complement(870..1355)
                     /locus_tag="VGM72_13220"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY4266274.1"
                     /translation="MSKRKVPSSVTRRSLLKGAALTGAAAATAPLPLRAQTAAPTRSV
                     SPPDPEVENYRVDGSPIVQTTSGSDFMLDVLKTMDFEYAAATCGSSFKGLHESVLNYG
                     KNTNPQWLATTHEETSVAIAHGYAKLENKPMLVCAHGTVGLQHAAMAVYDAYCDRVPC
                     S"
     gene            1498..>1915
                     /locus_tag="VGM72_13225"
     CDS             1498..>1915
                     /locus_tag="VGM72_13225"
                     /inference="COORDINATES: protein motif:HMM:NF022463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2848 family protein"
                     /protein_id="HEY4266275.1"
                     /translation="MSGQVLRLQLHAGAPAVRASEVAILHAVIAGWTGRDADAVEKHI
                     KELEVLGVKRPAATPIFYRVAASRLTLAASIEVLGTQSSGEVEFVLLQAEGVLWIGTG
                     SDHTDREVEAYGVSVSKQMCEKPVAPQFWRFSDVAAH"
BASE COUNT          321 a          584 c          638 g          372 t
ORIGIN      
        1 ggggctcgcg tcccagccat aggcggcctc cgtgcgtacg cgctcgagtg ccgtcttgtg
       61 cgcctcgccg agcttttgcc cccgcgcggc gaacgcggct ttgcgcccgc cgtccatcgc
      121 gcgtttcacc gcttcgatca gcaccggcat ggtggttgcc gcatccgccg caatggcgag
      181 gtcgaccggt tcgaagcgct ggaagttctg gaaattggac ttgatgaaca aatcggccga
      241 actgatgctg atgatcttcg cgccgggctt ggtggttgct cgcgagctgg agacgatatg
      301 ttcgcggaac tgatgggtga cgccccagaa atccaccact tcgagcccca gcaccacatc
      361 ggcctgtccg accgtgccgc gcccgccacg gctggtctgg ttcaacggat ggcgcgtggg
      421 aaaattcatt cgcgcggaca catcgatgac gccgcattgc agaagctccg ccagctccac
      481 cagggaatcg acggctgctt gcgaacggcc ataggattcc gcgacgatga ccggattctc
      541 tgcggcaacc agcagtttcg ccacttcggc gatggcgccg gattcgcctt gcggcatcgc
      601 ggtcacgggc agtttgggga tggtgggctt ttcgccctcg gggatggggc cttcctgcat
      661 ctcggtgtcg caggccagga tcaccggccc catcggcggc gtggtcgcga tcttgtaggc
      721 gcggaccgcg gattcggcga aatgctggag cgaggcgggg cggtcgtccc atttgacgaa
      781 atcgcgcacc atggcgacat cgtcctgcga ggaatgcacc caatggaccg cgttttcgcg
      841 cgccgcggcg tcgaacgtgt tgccgacgat catgaacacg gcacgcgatc gcaatatgcg
      901 tcatagaccg ccatcgcggc gtgctgcagc ccgaccgtgc catgggcgca caccagcatc
      961 ggcttgtttt ccagcttggc ataaccatgg gcaatggcga cggaggtttc ctcatgcgtg
     1021 gtggcaagcc attggggatt cgtgttcttg ccgtaattca agaccgattc atgcaggccc
     1081 ttgaagctcg acccgcatgt ggcggccgca tattcgaaat ccatcgtctt caggacgtcg
     1141 agcatgaaat cggaaccgga cgtcgtctgc acgataggac ttccgtcgac gcggtaattc
     1201 tcgacttccg gatcgggcgg cgagacgctg cgcgtgggtg cggcggtttg cgcgcgcaag
     1261 ggaaggggcg ctgtggccgc ggcggcgccg gtgagcgcgg cacctttaag aaggcttcgg
     1321 cgcgttaccg aagacggcac cttacgcttg gacatcctta tcccctcaac ctgacgccgg
     1381 acccggaggg taggccggtc ctcaggccct ggaaagagcg gcgcgggcgc gatttgaccc
     1441 ttgccttcaa agcgtgcgac acggctcacg ccgaaagtct gcgtttgtga ggggatcttg
     1501 tccggacagg ttttgcgttt gcaattgcac gctggagcgc cggccgtccg cgcgtccgag
     1561 gtcgccatcc tgcacgcggt gattgccgga tggacgggac gcgatgccga tgcggtggag
     1621 aagcacatca aggaattgga agtgctcggc gtgaagcgtc ccgccgccac gccgattttc
     1681 tatcgcgtgg cggcgtcgcg gctgaccctg gctgcgtcca tcgaggtgct cggcacgcag
     1741 tcgagcggcg aggtggaatt cgtgctgctg caagctgaag gcgtgctctg gatcggcacc
     1801 ggctcggatc acaccgaccg cgaggtggaa gcctatggcg tttcggtctc caagcagatg
     1861 tgcgagaagc cggtcgcgcc ccaattctgg cgattttcgg atgtcgctgc ccatt
//
LOCUS       DASYEG010000308         5238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2731968, whole genome shotgun sequence.
ACCESSION   DASYEG010000308 DASYEG010000000
VERSION     DASYEG010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2731968"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VGM72_13230"
     CDS             complement(<1..255)
                     /locus_tag="VGM72_13230"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY4266276.1"
                     /translation="MNRGALPLDSNADAPAENDVPDHLSEQMALYGSDEHFRGVLEAL
                     PAAVYMTDAQGRITFYNKAAHELAGREPKLGSDEWCVTWRL"
     gene            complement(392..1261)
                     /locus_tag="VGM72_13235"
     CDS             complement(392..1261)
                     /locus_tag="VGM72_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266277.1"
                     /translation="MHRSVPVACLLMASLVLGEGKGNGAEAVPDLSGVWGRNTIDYIA
                     PETGKGPVKNISGSRNMMVGDFHDPMLKPWAADIVKRNGEIAKTGDAFPTAHNQCWPE
                     PPPYILGNQQLQILQQRDQVTLMYSHSSQVRRVRLNATHPANVMPSWYGDSVGHYEAD
                     ALVVDTVGVTVAPLSSLDRYGTPHTEALHVVERYHMVEQTPTAAEPLRNGGFQGVNDD
                     VIDRNYKGKLLRVDFTVEDSGTFTMPWSGYVVYNRANGQFIEDVCAENIHDYVTGRDS
                     AVPTAKTTLLSGR"
     gene            complement(1278..2138)
                     /locus_tag="VGM72_13240"
     CDS             complement(1278..2138)
                     /locus_tag="VGM72_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266278.1"
                     /translation="MLVSPALPASPKDSIPDFSGFWARTSLGFESPAAGHGPLGNLNH
                     RANGASNLSLLAGDDKDPILKPQAAEVVRRLAEWGRRNEAFPQPSNQCLPYPPPYIAS
                     NNQEVELIQLKDRVLILTMFDHQIRRVRLNAQHPAELTPSWYGDSVGHYEGDTLVIDT
                     VGIKPGPYPMTDMMGTPFSRQLHVVERFRLVDAAAAKAFAERGEAENGVVAGLNGDGV
                     EIDTAHQDKGLQLTVVVEDPGMLNASWSGTVTYRRPLLDWAESVCAENTREYYANRDT
                     TIPHADRPDF"
     gene            2485..3951
                     /locus_tag="VGM72_13245"
     CDS             2485..3951
                     /locus_tag="VGM72_13245"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY4266279.1"
                     /translation="MSASEAGLTLVQGGYGGLRGWIDRVEQMGELKRVHGASWDTEMG
                     AITHMLTEHSHGTAPALLFDDIPGYPKGYRTLYGHFSSVKRVALTLGLPLEHERKVDI
                     VKQYYNRIQTMKHIPPRYVQDGPILQNVIEGDAIDVLKFPVPRHHEMDKARYIGTANC
                     VITQDPDAGWFNLGTYRNQVYDGKTIGCQITEGKHGRIHRDKYFERGEKMKVVVLVGQ
                     DPLLFMLAASPLPEGVSELDFAGGLRGEPIDVIRGPYTGFPIPADAEIAIEAEAVPGM
                     VREEGPFGEWMGYYSDDTVPRPYLDIKTILHRNNPILTCAPQHKPVDETGLLKGIAGA
                     AQVWRALEACGLPDILGVWNHEAGPATRFTAIQIKQRYPGHARNVLHVACSCQGGAYA
                     GKWTVVVDEDIDAGDLDQVLWAMSTRFDPMTDIDLVQKAWASKRDPLYLPGNFNNRIL
                     IDACIPYDKLLKGTFPPVVDVSPKLRSTLKAKFPQLFA"
     gene            4103..5122
                     /locus_tag="VGM72_13250"
     CDS             4103..5122
                     /locus_tag="VGM72_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266280.1"
                     /translation="MMHLRFRSAAVIASAMLLGASIAQAATPAEFYKGRTISVIIGYS
                     AGGGYDLYARVLSQHLGKHIPGNPTVIPQNMPGAGSIKTANYIYSVAPKDGTVIGTFA
                     RGMASAALIGQAKLDARKFTWLGSMTKDVTLCISWNTSPIKNWNDAMTKQFTAGGEGA
                     AGDPDIFAKLYKNVFGAKIRLATGFPGTTDITLAMQRHEVDGLCGISWSTVKARYDNW
                     VKDKKINILIQAAPAKASDLPDVPLASEFAKTTEQRQILDFAVANEVLARPFVAPPGI
                     PTDRKNVLLAAFAATLRDPAFLADASKTMLDIDPVSGPEAEAVVASLYALPKDVVAKA
                     ARAIY"
BASE COUNT         1066 a         1605 c         1565 g         1002 t
ORIGIN      
        1 aagccgccag gtcacacacc attcatcgga cccgagctta ggctcacgcc cggcgagttc
       61 atgcgccgct ttgttgtaaa aggtgatgcg gccttgcgcg tcggtcatgt agaccgcagc
      121 gggaagcgct tcgagcacac ccctgaaatg ctcatcgctg ccgtacaatg ccatctgttc
      181 agacagatgg tccggaacgt cgttctccgc cggcgcgtcc gcgtttgaat ccaaaggaag
      241 cgcaccccga ttcatcttcc ctcacgcgca ataataggtg cgaacaatat aagggccctt
      301 gtgcaaggcc ccaaggggtg ggttgtgagg aattcgaaac atcccattca agggcagcca
      361 ttcgttggag ccaatcagcc gtgggagcca atcagcgacc ggaaagcagc gtcgtcttgg
      421 ccgtgggcac cgcggaatcg cgccctgtca cgtagtcatg gatattctcg gcgcaaacat
      481 cctcgatgaa ctggccgttg gcgcgattgt aaaccacgta tcccgaccat ggcatggtga
      541 atgtgccgga atcttccacc gtaaaatcca cgcgcagtag ctttcccttg tagttgcggt
      601 cgatgacatc gtcattgaca ccctgaaatc cgccattgcg aagaggttcg gcggcggtgg
      661 gtgtttgctc caccatgtgg taacgctcca caacgtgcag cgcctcggta tggggcgtcc
      721 cgtagcgatc cagactggaa agcggcgcaa ccgtgacgcc aaccgtatcg accacgagcg
      781 cgtcggcctc gtaatggccg acggaatcgc cgtaccagga cggcatcacg ttcgcgggat
      841 gcgtcgcgtt cagacgcacg cggcggacct gcgaactatg cgagtacatc agcgtgacct
      901 gatcccgctg ctgcaggatc tgcaattgct gattgccgag gatgtagggc ggcggttcgg
      961 gccagcattg attatgggcg gttgggaatg catcgccggt tttggcgatt tccccattgc
     1021 gcttgacgat gtcggccgcc cagggcttca gcatgggatc gtgaaaatca cccaccatca
     1081 tattgcggct gccggagatg tttttgaccg gccccttgcc ggtttccggc gcgatgtaat
     1141 cgatggtgtt gcggccccaa acgccggaca gatcgggaac cgcctccgcc ccattccctt
     1201 tgccctcacc gaggacgagc gatgccatga gcaggcacgc gaccggcacg gacctgtgca
     1261 tcgttgttcc cttccgccta aaagtccggt ctatccgcat gcgggatcgt cgtgtcccgg
     1321 ttggcataat attcgcgcgt gttttcggcg cagacgcttt ccgcccaatc cagcaacggc
     1381 cggcgatagg tgacggtccc cgaccacgaa gcgttcagca tgccggggtc ttcgaccacg
     1441 acggtaagct gcaagccttt gtcctgatgc gccgtgtcga tctccacgcc atcgccattc
     1501 aaacccgcga caacgccgtt ttcggcttcg ccgcgttcgg cgaatgcttt ggccgccgcg
     1561 gcatcgacga gacgaaaacg ctcgaccaca tgaagctgcc tgctgaacgg cgtgcccatc
     1621 atatcggtca tcggatacgg cccgggcttg atgccgaccg tgtcgatcac caacgtgtca
     1681 ccctcataat gaccgacgga atcgccatac caggatggcg tcaattccgc gggatgttgg
     1741 gcgttcagcc gcacgcggcg tatctggtgg tcgaacatcg tgagaatgag cacccgatcc
     1801 ttgagttgga tcagctcgac ctcctgattg ttcgatgcga tgtaaggcgg cggatacggc
     1861 aggcactgat tggaaggctg cggaaaggcc tcgttcctgc gaccccactc ggcgagcctt
     1921 ctcaccactt cagccgcctg aggcttgagg atggggtctt tgtcgtcccc tgcgagcagg
     1981 ctgagattgg aagcgccatt ggcgcgatgg ttcagattgc caagcggacc atggccggcc
     2041 gccggacttt caaacccgag cgaggtgcga gcccagaaac cggagaaatc cggaatggaa
     2101 tccttcggcg acgccggcag cgctggcgag acaagcatcg tcgcagagaa cacccctgca
     2161 atcagagcac tggctctcct atccggaatg ccgcccgcca tagactcctc cgtcgtcagc
     2221 tatgacttcc gctgacacaa tatattgcag gttgcgtgcg gccgcgcgag ccattcccgc
     2281 agactttggg atgacggacg ttgcgtcacc gcgcccggaa aaatccgcgc cttggcggtc
     2341 tctatcgcag cgatgcggcc ctggcgattt gagagcgcac cacacgtcta tgtgcgtaga
     2401 agcttgcact tctgcgtccg atttaggata gtcttcgtac caaaatcaat ccaaatccaa
     2461 attttggtac agtctggagg gtccgtgtcc gcgagcgaag caggactgac gttggtgcag
     2521 ggcggttatg gcggtctcag gggctggatc gaccgggtcg agcagatggg ggagctcaag
     2581 cgcgtccacg gcgcaagctg ggacaccgag atgggcgcca tcacccatat gctgaccgag
     2641 catagccatg gcaccgcacc ggcgctgctg ttcgacgata tcccgggtta tccgaaaggc
     2701 tatcgcacgc tctatggaca tttctcatcc gtgaagcgcg tggccctgac gctcggcctg
     2761 ccgctggaac acgagcggaa agtggatatc gtcaagcaat attacaatcg catccagacg
     2821 atgaagcaca tcccgccccg gtacgtgcag gacggtccga tccttcagaa cgtgatcgag
     2881 ggcgatgcca tcgacgtcct gaaatttccg gtgccgcgcc atcacgaaat ggacaaggcg
     2941 cgctacatcg gtaccgccaa ttgcgtcatc acccaggacc cggatgcggg ctggttcaat
     3001 ctcggcacgt accgcaacca agtctatgac ggcaagacca tcggctgtca gatcaccgag
     3061 ggcaaacacg gacgcatcca ccgcgacaaa tatttcgaac gcggcgaaaa gatgaaggtg
     3121 gtggtgctgg tggggcagga tccgctgctg ttcatgctgg cggcgagccc cctccccgaa
     3181 ggcgtcagcg aactggattt cgccggcggc ttgcgcgggg agcccatcga cgtgatccgc
     3241 gggccctaca ccggttttcc gattccggcc gatgcggaaa tcgcgatcga ggcagaggcc
     3301 gtacccggaa tggtgcgcga ggaaggcccg ttcggggaat ggatgggcta ttattccgac
     3361 gacacggtgc cccggcccta tctcgacatc aaaaccatcc ttcaccgcaa taaccccatt
     3421 ctcacctgcg cgccgcagca caaaccggtg gatgagacgg ggttgctgaa aggcatagcg
     3481 ggtgccgccc aagtgtggcg cgcgctggaa gcctgcggcc tgcccgacat cctgggcgtg
     3541 tggaaccacg aggccggtcc tgccacgcgc ttcacggcga tccagatcaa gcagcgttat
     3601 cccggtcatg cgcgcaacgt gctgcacgtg gcctgttcgt gccagggcgg cgcctatgcc
     3661 ggcaaatgga cggtcgtggt ggacgaggac atcgacgcgg gcgacctcga ccaggtgctg
     3721 tgggcgatgt cgacacgctt cgaccccatg accgacatcg atctggtgca gaaggcctgg
     3781 gcgtccaagc gcgatccgct ttatctgccg ggcaatttca acaatcgcat cctgatcgat
     3841 gcgtgcatcc cgtacgacaa attgctgaag gggacattcc cgcctgtcgt cgatgtcagc
     3901 ccgaaactgc gcagcacgct gaaggcgaaa ttcccgcaat tgttcgcata gatcggacag
     3961 gcgccgaaat gagacacgaa gaccaaggac gcaccaggat cacaaaatgt tttgtccgga
     4021 gcggcggctg ggaaacgtct ccttttttgc cgggttcttc gtgtatcctt cgtgtccttt
     4081 gtatttgccc gattgcggac acatgatgca tttgcgcttc cgctctgccg ccgtcatcgc
     4141 ctccgcgatg ctattgggcg caagcatcgc acaggcggcg acgccggcgg aattttataa
     4201 aggccgcacc atctcggtga tcatcggcta ctcggcagga ggtggctacg acctctatgc
     4261 gcgcgtgctg tcgcagcatc tgggcaagca cataccgggc aatcccaccg tcattcccca
     4321 gaacatgccg ggagcgggca gcatcaagac cgccaattac atatattcgg tcgcgcccaa
     4381 ggacggcacc gtcatcggaa cctttgcgcg cgggatggcc agtgcggcgc tcatcggcca
     4441 ggcgaaactc gacgcacgca aattcacctg gctcggcagc atgacgaagg acgtcacgct
     4501 gtgcattagc tggaacactt ctcccatcaa gaattggaat gacgcgatga cgaagcaatt
     4561 caccgccggc ggcgaagggg cggcaggcga tccggacatc ttcgccaagc tctacaagaa
     4621 cgtgttcggc gccaagatcc ggcttgccac gggttttccc ggcaccacgg atatcacact
     4681 cgccatgcag cgccatgaag tcgatgggct atgcggaatt tcctggagca ccgtcaaggc
     4741 gcgctatgac aattgggtga aagacaaaaa gatcaacatc ctcattcagg ccgcaccggc
     4801 aaaggcctcg gacttgcccg atgtgccgct cgcgagcgaa tttgccaaaa cgacagagca
     4861 gcggcagatc ctcgatttcg ccgtcgccaa tgaggtcctc gcgcgcccct tcgtagcgcc
     4921 gccggggatt cccaccgacc ggaagaatgt gttgctcgcc gcgttcgcgg ccacgcttag
     4981 agatcccgcc ttccttgccg atgccagcaa gacgatgctc gacatcgatc ccgtcagcgg
     5041 ccccgaagcg gaagccgtgg tcgcttcgct ctatgcgctt cccaaggacg tggtcgcgaa
     5101 agccgcgcgc gcgatctatt gagcgcaaaa ctttatgacg gagaggaggg ccgcatcggc
     5161 cagcgcttcc ggcggctgga agatttgccg ctgctcctgg ggcggggacg ctttgtcgac
     5221 gacatccgga ttccgggc
//
LOCUS       DASYEG010000309         2516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_5048851, whole genome shotgun sequence.
ACCESSION   DASYEG010000309 DASYEG010000000
VERSION     DASYEG010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5048851"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VGM72_13255"
     CDS             <1..183
                     /locus_tag="VGM72_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266281.1"
                     /translation="ILLAGTGPGKGAPGDVTFDFAIVCGEGALRPLLVQRAGRAPADW
                     LSFLRGFALKPGERVA"
     gene            183..923
                     /gene="truA"
                     /locus_tag="VGM72_13260"
     CDS             183..923
                     /gene="truA"
                     /locus_tag="VGM72_13260"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY4266282.1"
                     /translation="MPRYRVTLEYDGGPFAGWQRQDNGPSIQGALEEAIFRLAGERVT
                     VTGAGRTDAGVHARGQVAHFDLVKEFAPTTIRDALNAHLRPAPVAIVEAALSAADFHA
                     RFSAIARHYEYRILTRRAPPALDRGHVWHVGRPLDVAAMQEGARAFIGQHDFTTFRAA
                     ECQAKSPVKTLDRFDVSEQGEEIVCITRARSFLHHQVRSMVGTLKLVGEGKWCAADVT
                     HALRARDRTQCGTVAPADGLTLLRVDYS"
     gene            complement(949..1800)
                     /gene="murI"
                     /locus_tag="VGM72_13265"
     CDS             complement(949..1800)
                     /gene="murI"
                     /locus_tag="VGM72_13265"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEY4266283.1"
                     /translation="MTQDSRDCPIGVFDSGMGGLTVMRALMTRLPHESFVYLGDTARL
                     PYGSKSPDTVTRYAVQCARALMAHRIKLLVVACNTASASALPALTAVLAPTPVIGVIE
                     PGADAAIAAAPSGRIAVIATEGTVKGGAYERAIHARDPRARVTQQACPLFVALAEEGL
                     TDGPIAELVARRYLDPLLSAADTRPECLVLGCTHFPALKDTIARIAGPGIALVDSADT
                     TAAAVERLLAKEQLSRPDESPTAPPRFLATDAPERFARVGQIFLGRMIPADAVDQVDL
                     HPGSRSA"
     gene            complement(1797..>2516)
                     /gene="dapE"
                     /locus_tag="VGM72_13270"
     CDS             complement(1797..>2516)
                     /gene="dapE"
                     /locus_tag="VGM72_13270"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF009557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEY4266284.1"
                     /translation="TVKVLDWLKQNGEALDHCLVGEPTSAARIGDMMKIGRRGSMNLR
                     ASVKGIAGHAAYPHLALNPIPIMADFVARISAWRLDEGTEHFEPSTLAFTTVDVGNPT
                     VNVIPGEVRAGFNIRFNDAHTPDSLMARIGEIADAVRATHGGEIVLSKSVSGVSFLTK
                     PGAYTELLSKAAQSVTGIAPELSTTGGTSDARFIKDHCPVAELGLPGRTMHKIDECVS
                     IADIETLTRIYEAVLHLYFTQ"
BASE COUNT          430 a          789 c          850 g          447 t
ORIGIN      
        1 attcttctgg cggggacagg accgggaaag ggcgccccgg gcgatgtgac gttcgatttc
       61 gccattgtct gcggcgaggg tgcgctcagg ccgcttctgg tacagcgcgc gggccgtgcg
      121 cctgccgatt ggctctcctt cctgcgcggc tttgcgctca aacccggcga gcgcgtcgcc
      181 tgatgccgcg ctatcgcgtt acgctcgaat atgatggcgg tccgtttgcc ggctggcagc
      241 ggcaggacaa tggcccttcg atccagggcg cgctggaaga ggcgattttt cgactcgcgg
      301 gcgagcgtgt cacggtgacg ggggcgggcc gcaccgatgc cggcgtgcat gcgcgcggcc
      361 aggttgccca tttcgatctg gtcaaggaat ttgccccgac caccattcgc gacgcgctga
      421 atgcgcatct gcgcccggca cccgttgcga ttgtggaggc ggcgctttcg gcagccgatt
      481 ttcatgcgcg attttcagcg attgcacggc actatgaata tcgcatcctc acccggcggg
      541 cgccgcccgc cctcgacagg ggacatgtct ggcatgtggg gcggcccctc gacgtcgcgg
      601 ccatgcaaga gggcgcgcgt gccttcatcg ggcagcatga tttcaccacc ttccgcgctg
      661 ccgaatgcca ggcgaaatcg ccggtgaaga cgctcgaccg ttttgacgtg tcggaacagg
      721 gcgaggagat cgtctgcatc acgcgtgcgc ggtccttcct gcatcatcag gtgcgctcca
      781 tggtgggcac gctcaagctg gtcggcgagg ggaaatggtg cgccgccgat gtgacgcatg
      841 cgctgcgagc gcgcgaccgc acccaatgcg gcaccgtcgc gccggccgat ggattgacgc
      901 tgctgcgcgt ggactattcc tgagagactg tcatcccggc cagcgcagct aagcggagcg
      961 cgagccggga tggagatcga cctgatcgac ggcatcggca ggaatcatcc gcccaaggaa
     1021 tatctggccc acgcgggcga aacgttccgg cgcgtcggtt gcaaggaagc gggggggagc
     1081 cgtgggtgat tcatcaggac gcgaaagctg ttccttcgcc agcaagcgct cgacggcggc
     1141 ggccgtggta tcggcgctgt ccacgagcgc gatgccggga cccgctatgc gcgcaatggt
     1201 gtctttcagc gccggaaaat gcgtacagcc gagcaccagg cactccggac gcgtatccgc
     1261 tgcgctcagc aagggatcga gataacgcct ggcgaccagt tcggcgatgg gtccgtcggt
     1321 caggccctct tcggcgagcg caacgaacaa tgggcaggcc tgctgcgtca cccgcgcgcg
     1381 cgggtcacgc gcatggatgg cgcgttcata agcgccgccc ttgaccgtgc cttcggtcgc
     1441 gatcaccgcg atgcggcccg aaggtgccgc ggcaatcgcg gcatccgcac ccggctcgat
     1501 cacgccgatg accggggtcg gcgcgagcac cgcagtgagc gccggcagcg cgctggccga
     1561 tgcggtattg catgcgacca cgagcagctt gatccgatgc gccatcaaag cgcgcgcgca
     1621 ctggacggcg tagcgcgtga ccgtgtcggg gcttttgctg ccatagggaa gccgcgccgt
     1681 gtcgccgaga tagacaaaac tctcatgcgg caagcgcgtc atcagcgcgc gcatcacggt
     1741 gagcccgccc atgccggaat cgaacacccc gatgggacaa tcgcgcgaat cttgcgtcat
     1801 tgcgtgaaat agaggtggag caccgcttcg tagatgcgcg tgagtgtctc gatatcggcg
     1861 atgctcacgc attcatcaat cttgtgcatg gtgcgtcccg gcaggccaag ctcggcaacg
     1921 ggacaatgat ccttgatgaa gcgcgcatcc gacgtgccgc cggtcgtcga aagctccggc
     1981 gcgataccgg tgacgctttg agcggctttg ctcaaaagtt cggtataggc accaggcttg
     2041 gtcaggaacg acacgccgct gacgcttttg gaaagcacga tctcgccgcc atgggtggct
     2101 ctcacggcat cggcgatttc cccgatccgg gccatcagcg aatccggcgt gtgggcgtcg
     2161 ttgaagcgga tgttgaagcc cgcccgcact tcgcccggaa tcacattgac ggtggggttg
     2221 cccacatcga cggtggtgaa ggcgagcgtg gagggttcga aatgctcggt gccctcgtcc
     2281 agccgccacg cggaaatccg cgccacgaaa tcggccatga tgggaatggg gttgagcgcg
     2341 agatgcggat aggccgcgtg gcccgcgatg cccttcacgc tcgcgcgcaa attcatgctg
     2401 ccgcggcggc cgatcttcat catgtcgcca atgcgtgccg cggaggtcgg ttcgccgacc
     2461 aggcaatggt ccagcgcttc gccgttctgt ttcagccaat ccagaacctt cacggt
//
LOCUS       DASYEG010000310         2738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4035652, whole genome shotgun sequence.
ACCESSION   DASYEG010000310 DASYEG010000000
VERSION     DASYEG010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4035652"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            406..1275
                     /locus_tag="VGM72_13275"
     CDS             406..1275
                     /locus_tag="VGM72_13275"
                     /inference="COORDINATES: protein motif:HMM:NF018691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY4266285.1"
                     /translation="MAVASQKGGSGKTTLSGHLAVEAERAGAGPVTLIDTDPQGSLSQ
                     WWNARVARAPQFAKVGAFELQSALNHLSRTGTRLAIIDTPPAISDSISHVIAHADMVI
                     VPTRPSPHDLRAVGATVDMAERFGKPLIFVLNGATLRARITGEAAVALSQHGVVAPVF
                     IHHRVDFAASMVDGRTVGEAVPKSASAQEMTQLWTYVQDRISRLVKETNSEVHANHFS
                     LAAATEEFDNIEMPLLTAGSPANAASFEAPENRSGVDRRDPPRPGTFAGPERRTGVFG
                     RRNSDQHDMVGRK"
     gene            1425..1748
                     /locus_tag="VGM72_13280"
     CDS             1425..1748
                     /locus_tag="VGM72_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266286.1"
                     /translation="MRQSSKLRTVAPPEASKMPPPKSASPQPPRAAPAGDPPPGQPGK
                     TRRLTVSVSPSEYETLGLVAVKKNVTRHQLVRSALDEYLALLVDEYGEGCRCIYTGCS
                     CGKQA"
     gene            complement(1761..2606)
                     /locus_tag="VGM72_13285"
     CDS             complement(1761..2606)
                     /locus_tag="VGM72_13285"
                     /inference="COORDINATES: protein motif:HMM:NF018420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HEY4266287.1"
                     /translation="MAAWNHWESASLGISILAVCLLGSTAGGVPAAEPVDVALVIATD
                     VSYSVDENEAQFQREGAIAAFRNPDVVKAIQSGSLGRIAVAYIDFSSYQSNKILVDWK
                     IVHDKASGDAFADALAAKPRTLGVQTSISSGLELAERLLETSGFNASKRVIDVSGDGP
                     NNDGHLVDKVRDEIVAKGIVINGLPIMTPADQFDVYYLPDLDKYYAGCVIGGPGAFLQ
                     VAHGFEDLARALRRKLIQEISDSGGRGNPLLVPVAAVSPRGSSPHAAYEKGCDIGERM
                     RFGGN"
BASE COUNT          515 a          864 c          832 g          527 t
ORIGIN      
        1 gtgtgtccaa gcttcattgg aatctccctc ttccaaaccc caaaattggt ggcatggtgc
       61 tgcgcgcctc gaagcacgca tgtgccgtta gccactatag cccattttcg ccgaatggtt
      121 agcgtcccct tagggcgctg ccgcgggcgc gtggcatgct gcggtgctcc catgaccctg
      181 gaacacccag gaatctcgcc gatttttcgg tgacctggcg catggttcac ggggatgtgt
      241 cggcgccggg ccgggggcct tggcccaaga ggcggggtct tggcatcgta atcgcaaaga
      301 aatcctgaaa aattcgatgc aattattggt cttcgccttt gcgcgatcga aagacctctc
      361 ccgtagaaag gctgagcatt ttctttggac ggtctcatga ttgtcgtggc ggtagcatcc
      421 caaaaaggcg gatcgggcaa aaccaccctg tccggccatc tggcagtaga agcagaacgg
      481 gcgggcgccg ggccggtcac gctgatcgat accgatccgc agggctcgct ttcgcaatgg
      541 tggaacgcgc gcgtggcgcg cgcgccgcaa ttcgcaaaag tcggcgcttt cgagttgcaa
      601 agcgcactga atcatctcag ccgcacaggc acgcggctcg cgatcatcga cacgccgccg
      661 gcgatttccg attccatatc tcatgtgatt gcccatgccg atatggtgat cgtgccgacg
      721 cgtccatcgc cgcacgatct tcgtgcggtg ggtgccacgg tcgacatggc ggagcgcttc
      781 ggcaaaccgc tgatcttcgt ccttaacggc gccaccttgc gcgcgcgcat taccggcgaa
      841 gcggccgtgg cgctgtcgca gcacggcgtc gtggcgccgg tattcatcca tcatcgcgtc
      901 gatttcgccg ccagcatggt tgacgggcgc acggtcggcg aggccgtgcc caaatcggcg
      961 tcggcgcagg agatgaccca gctctggacc tatgtgcagg accggataag ccgcctggtc
     1021 aaggagacga attccgaagt ccacgccaat catttctcgc ttgcggcggc caccgaggaa
     1081 tttgacaata tcgagatgcc gttgctaacc gccggttctc cggcgaacgc cgcctctttc
     1141 gaggcgccgg aaaatcgctc tggagtcgac cggcgtgatc ctccccgtcc gggaaccttt
     1201 gccggtccgg agcgccgcac cggcgtattc ggccgccgca attcggacca gcacgatatg
     1261 gtcggccgga agtgagcggg gggaaattcg caagtctgac ggcgcggctc atggtgcgca
     1321 aagaggcgag gccctcttcc atcatggcgc cggtattttc ctttccgcgc gggctggaac
     1381 agtttgggat atcgcggctg ccggaaaccg cgcctcaacc gtcaatgagg caatcgtcca
     1441 agctgcgcac ggtcgcgccg ccggaagcct ccaagatgcc gccgccaaaa tcggcatcgc
     1501 cacagccgcc ccgcgccgcg ccggccggcg atccgccacc gggccagccg ggaaaaacac
     1561 gccgccttac ggtatcggtc tcgccaagcg agtacgaaac gcttggactc gttgccgtga
     1621 aaaagaacgt tacgcgccat cagctcgtgc gcagtgcgct cgacgaatat ctggcgttgc
     1681 tggtcgatga atatggcgaa ggctgccgat gcatctacac cggctgttcc tgcggcaagc
     1741 aggcgtagcg ctttgatttc ttagtttccc ccaaagcgca tccgctcgcc gatatcgcag
     1801 cctttttcat aggccgcatg cggcgatgaa ccccgcgggc ttaccgcggc gacggggacg
     1861 aggaggggat tgccacgccc gcccgaatcg gaaatctcct ggatgagctt gcgccgcaaa
     1921 gcgcgggcga ggtcctcgaa gccgtgcgcc acctgaagga acgcgcccgg tccgccgatc
     1981 acgcagccgg cataatattt gtcgaggtcg ggcagataat agacgtcgaa ttgatcggcg
     2041 ggggtcatga tcggaagacc gttgatcacg atgcccttgg cgacgatttc gtcgcgcacc
     2101 ttgtcgacca gatgtccatc attgttcggt ccgtcgccgg acacgtcgat cacgcgtttg
     2161 ctggcgttga atccgcttgt ctcgagaagg cgctcggcca gctcgagccc gctgctgatg
     2221 gatgtctgga cacccagcgt gcgcggcttc gccgcgagcg cgtcggcgaa cgcgtccccg
     2281 ctcgccttgt catgcacgat tttccaatcg acgagaatct tgttcgactg atagctggaa
     2341 aaatcgatgt aggcgacggc gatccgtccc aacgatcccg actggatcgc cttgaccaca
     2401 tcgggattgc gaaacgccgc gatagcgccc tcgcgctgga attgcgcttc attttcatcg
     2461 acgctgtagg agacatcggt cgcgatgacg agcgccacat cgaccggctc ggcggcgggc
     2521 acaccgcccg cggtacttcc caacaggcac accgcgagga ttgaaatccc gagcgatgcg
     2581 ctctcccaat ggttccaagc ggccattcgt tacctctgcc gggcatccgc ttgggacaac
     2641 gatactcccg gctcgaagtt acggccagtg ccggaacgca gatgaaattt ttctcattgc
     2701 gtctacggaa cgccgcatcg cggccctcgc gaaaatcc
//
LOCUS       DASYEG010000311         2702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2587647, whole genome shotgun sequence.
ACCESSION   DASYEG010000311 DASYEG010000000
VERSION     DASYEG010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2587647"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VGM72_13290"
     CDS             <1..814
                     /locus_tag="VGM72_13290"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HEY4266288.1"
                     /translation="QFDRTGKFVREIGKDGYGFMFANQVRVDPQDNVWVVDQMTNMVI
                     KFDPQGHVAMLLGRKAESINIPARPAAGGGEGGGGAGGPVGAGRNQDIFDRPTDVAWD
                     AQGNIFVADGQGNARVAKFDKDGKFVKSWGHKGTGPSEFDNVRSIAVDAQGNVYVADG
                     NNKRIQVFDDNGTFKTQIANLGNPQSICITPGANQFLYVSNSNPPGDIDTAGEIYKMK
                     LDGTMVGKFGRAGKMPKEFGTVNAIDCRTDNNLLVGEIGNLRVQKVTLHPAG"
     gene            complement(912..>2702)
                     /locus_tag="VGM72_13295"
     CDS             complement(912..>2702)
                     /locus_tag="VGM72_13295"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY4266289.1"
                     /translation="AAIARRNYVLDQMYENSYITRDQMKAAKAEDLVTYNRPFGAVVA
                     DVDYFVEEVRRTLYGKYGEKALYDGGLQVRSTLDPRLQDIAVRSLRAGLLAYDRRHGF
                     RAPLKHLELKGSDWMAEFKAVPNDKSGVPSWRVAAVTELKGDATLMLQDGGTGKIPAA
                     ELAWTRGRLKAGDVVYVEPMSGEGVTPGSYTLREVPQVNGAIVAIDPFTGHVLALSGG
                     FSYRSSQFDRAMQAMRQPGSTFKPFVYATALDQGYTPITKVLDAPFAAPQGPGLPLWT
                     PENYETGEYLGPTTLRRGIELSRNLMTARLAHTIGMESIAATVERMGVYNKLPLYLAN
                     SLGAEVTTLLRLTTGYAEFVNGGRKLEATLIDRVQDRHGKTVYRFDKRNCAECSQPEW
                     KGQEEPLLDENREQVLDPRTAYQIVSILEGVVQRGTGVVIRAVGKPLAGKTGTSSDFR
                     DAWFVGFSPDLALGVFVGKDNFQTLGNGEQGALAAAPIFRDFMKEALADQPGTPFRTP
                     QGIVQVPINPITGAVVQLGTPGSILEAFKPGTEPGANYAASDIDDPGVAVPASASAGD
                     GLAAATPAAATAAGPPPPVTAIGTGTGGLY"
BASE COUNT          528 a          832 c          852 g          490 t
ORIGIN      
        1 ccagttcgac cggaccggaa aattcgtccg cgagatcggc aaggacggct atggcttcat
       61 gttcgccaat caggtgcgcg tggaccctca ggataatgtc tgggttgtcg atcagatgac
      121 caacatggtg atcaagttcg atccccaggg gcacgtggcg atgctgctcg gtagaaaagc
      181 cgagtccatc aacattcccg cgcggccggc ggccggcggc ggtgaaggcg gcggcggggc
      241 cggcggtccg gtgggcgccg gacgcaatca ggatatcttc gaccgaccga ccgatgtggc
      301 ctgggacgcg cagggcaata tcttcgtggc cgacggccag ggaaatgccc gcgtcgccaa
      361 attcgacaag gacggcaagt tcgtgaagag ctggggccat aaaggaacag gcccgagcga
      421 gtttgacaat gtccgcagca tcgcggtcga tgcccagggc aacgtctatg tcgcggacgg
      481 caacaataag cgcatccagg tgttcgacga caatggcacc ttcaagacgc aaatcgccaa
      541 tcttggcaat ccgcagtcga tctgcatcac ccccggcgcg aaccagttcc tctacgtctc
      601 caactccaat ccgccgggcg acatcgatac ggccggtgaa atttacaaga tgaaattgga
      661 tgggacgatg gtcggcaagt tcggccgcgc cggcaaaatg ccaaaggaat tcggcacggt
      721 gaatgccatc gactgccgca ccgacaacaa tctcctggtc ggcgagatcg gtaatttgcg
      781 ggttcagaaa gtgacgctgc accccgccgg ctgaaccgtt gttccacgaa ttatcgaaaa
      841 aaggcccggc tcgtccgggc ctttttgttt aggtcttttt attcggcgct aagtcgttcg
      901 gcctgaccgg attaataaag cccgccggtg cccgtgccga tggcggtgac ggggggcggc
      961 ggcccggcag cggtggccgc ggcaggtgtt gccgccgcca gtccatcacc cgccgacgcg
     1021 gatgccggca cagccacacc cggatcgtcg atatcggacg cggcgtaatt cgccccgggt
     1081 tcggtgccgg gcttgaacgc ttccaggatc gatcccggcg ttcccaattg caccaccgca
     1141 ccggtgatcg gattgatcgg cacctgcacg atgccctggg gcgtgcggaa cggcgtcccc
     1201 ggttgatcgg ccagcgcctc tttcatgaaa tcgcggaaaa tgggagccgc cgcgagcgcg
     1261 ccctgctcgc cattgccgag cgtctggaaa ttgtccttgc cgacgaagac accgagcgcg
     1321 aggtcgggcg aaaatcccac gaaccacgca tcgcggaaat cgctcgaggt cccggtcttc
     1381 ccggccagcg gcttacccac ggcgcggatc acgacgccgg tgccgcgctg caccacaccc
     1441 tcgaggatcg aaacaatctg ataggcggtg cgcggatcga gcacttgctc acgattctcg
     1501 tccagcagcg gttcttcctg gcccttccat tcgggctgac tgcattccgc gcaattgcgc
     1561 ttgtcgaaac gatagaccgt cttgccgtgg cggtcctgca cgcggtcgat cagggtcgct
     1621 tccaatttgc gcccgccatt gacgaattcg gcatagccgg ttgtcagacg cagcagcgtc
     1681 gtcacttcgg cgccgagcga attggcgaga tacagcggca gcttgttgta gacacccatg
     1741 cgctcgaccg tggcggcgat ggactccatg ccgatggtat gcgccagccg cgccgtcatc
     1801 agattgcgcg acaactcgat gccgcggcga agcgtggtgg gaccgagata ttcgcccgtc
     1861 tcatagttct caggcgtcca tagaggcagg ccaggtccct ggggtgccgc gaatggcgca
     1921 tcgaggactt tggtgatggg tgtgtagccc tgatccagcg cggtcgcata aacgaaaggc
     1981 ttgaaggtcg aacccggctg gcgcatggcc tgcatggcgc ggtcgaattg ggacgatcgg
     2041 taggagaagc cacccgagag cgcgagaaca tggccggtga agggatcgat ggccacgatg
     2101 gcgccgttca cttgcggcac ttcccgcaag gtatacgagc caggcgtaac gccttcgccc
     2161 gacatcggct cgacatagac gacatcgccc gccttcaggc gaccgcgcgt ccaggcaagc
     2221 tccgctgcgg ggatcttgcc ggtgccgcca tcctgcagca tcagggtggc atcgcccttg
     2281 agctcggtca cggcagccac gcgccagctc ggcacgcccg atttgtcgtt gggaacggcc
     2341 ttgaattcgg ccatccaatc actgcccttc agctcgaggt gcttgagggg cgcgcgaaag
     2401 ccgtgacggc ggtcataggc aaggagccca gcgcgcaatg agcgtactgc gatgtcctgc
     2461 agccgcggat cgagggtgga acggacctgc agaccgccgt catagagcgc cttctcgcca
     2521 tattttccat aaagcgtgcg gcgaacttcc tcgacgaaat aatccacatc ggcgaccacc
     2581 gctccgaacg gacgattgta agtgacgagg tcttcggctt tcgccgcctt catctggtcg
     2641 cgggtgatgt agctgttctc atacatctga tcgagaacgt aattgcgccg cgcgatggcc
     2701 gc
//
LOCUS       DASYEG010000312         6047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_268208, whole genome shotgun sequence.
ACCESSION   DASYEG010000312 DASYEG010000000
VERSION     DASYEG010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6047
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268208"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1392)
                     /locus_tag="VGM72_13300"
     CDS             complement(<1..1392)
                     /locus_tag="VGM72_13300"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4266290.1"
                     /translation="MADPRMQDARRLHEAGRLLDAARLYQRILHDDPKHFEALYGLGM
                     AHAQTGRLNEGERTVGEALARNPGFAEGWRARGLMLMHLGRGEDALTCFDSALAIEPD
                     FADAQSARAAVLAELTESRNRLATVETGLARDPNDAARWNSRGSLLAGMGRVQDALAS
                     FDRALSIHPGFVEALCNRATLLLETGKLDMALAGFDAVLEIESELAIGWNNRGNTLAR
                     MGRFEEAVASYDRALAIGPEFPEAVENRDFALFTLGRNARSPAKYMRGLFDEFSTHYD
                     HTMLEKLQYRAHLHVRAMAMRVLPRLCPSWRILDLGCGTGLVGLAFQDLASGGWIDGI
                     DLSPRMVEAARARGIYRDLILGDLETVLAEPGMNYDLIVSADTMTYFGDLAPVFSGVA
                     KCLAPGGFYLFASEAKTGAGWEQTKVRRFRHSEAYLRAEIARAGLEFVDIVECALRQE
                     ENEPVFGFTVAVKK"
     gene            complement(1405..3573)
                     /locus_tag="VGM72_13305"
     CDS             complement(1405..3573)
                     /locus_tag="VGM72_13305"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4266291.1"
                     /translation="MFRTYLRNSVSFAALLVSSAALAQQNPMVAANAAGSVPDNSIFN
                     LGQIEQVKVTASPMAATISESTVSAEEAFKFNALTVDRALDLVTGVASGTTGGPRNEK
                     LFFIRGFDRFQSPLFVDGIRVYLPADNRLDLGFFNTANLAQIQVEKGYVSVLSGPGAI
                     GGALNLVTRKPTQPFEYEARAGIQLGGNGDYNGYNTSGLVGGATDKYYWQASGGVTTT
                     DQFRLPDKFTPTATEDGGDRDHSDARNYNMNLKIGSTPNATDEYSLSYSGQWGRKDAT
                     LSVSDPVSAQKDWSWPYWDVQSLYFLSNTKVGDSAYVKTKVYYNSFRNGLYSYDNANY
                     NSQTTSKAFQSFYSDYAYGGSVEAGNDFGGRDILKGALFYRRDNHVEWQTIFVPKFTE
                     PHQQDLEDTYSAAVENRLHVTDQIDFVAGASYDWRHLIVAQDYNDPTAKTSGSFVNFP
                     LADGHADNAQAALIYNYDDTGHVYANVSDRSRFPTIFERFSTRFGSTLSNPNLKAERA
                     INYEIGGGDTFFGTRLNAAVFYSDVTNALENVPINFCDTTSTTAKDCSGAGGLSGTNT
                     VTSQTQNVGDGKYYGFEFSADTTITETLTAGVRYTYINRNIDAQNPINPPLPTNFHLT
                     GLPYSQVFAYVTWNVTQQISITPNIEAASDRWSNLSGNANSFLKTGSFVLLNLEADYA
                     FTEKIDFQIGARNLFDENYQLTAGFPSEGRNFFINLRYRS"
     gene            complement(3607..4968)
                     /locus_tag="VGM72_13310"
     CDS             complement(3607..4968)
                     /locus_tag="VGM72_13310"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEY4266292.1"
                     /translation="MIRRNVLTLGIFCLAAGGLANAAADTDVTVENRGRAAFQQPMPF
                     LDSAGVERFRAGASIFRQPWLVAPSDEQPRFDGLGPLSNRLSCAGCHIGNGRGETSAG
                     ENDVLRSSLVRLSVPGKAAMGAPAPEPVYGDQLQPIGIQGVPSEGDATIGWHETEQTL
                     DDGTRISLRRPELIFKNLHYGPFAQGTMTSVRMAPAIFGLGLLEAVPEAELKGLADPS
                     DANHDGVRGRLNRVWDVASGRIAIGRFGLKDNQPNLGQQVAAALIGDMGITSHLFREQ
                     NCTVAEKTCAGAAKGGDPEISDADFATLVFSIEALAPPAPKPAGAAAKDGQALFQNIG
                     CAACHVPSLTTGDSPQFPQAAHKTIHPYTDLLLHDMGEDLSDGRPDFDASGRDWRTTP
                     LWGFGLASTVVTHPAFLHDGRARDVTEAILWHGGEAQHARDRFASLSATDRKAIVEFL
                     QTL"
     gene            complement(4965..>6047)
                     /locus_tag="VGM72_13315"
     CDS             complement(4965..>6047)
                     /locus_tag="VGM72_13315"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY4266293.1"
                     /translation="IDVYWAPALFNFPALAQSGTFEKFQVDRSQVPGHIGAEAISDSN
                     GYFEAFEVAGYGIVTNPAVLKTKGIASPKTWDDLARPEYSGLLVMPVASRIGFSPQLY
                     DVILQAKGWDRGWALLSEMAANATLTAKGGEIADDVADGKAAAAIAIDFLIRNAISDG
                     KSVSLVYPAQTAFLPAYAAVVASSPHAQAARAFASFLVSDSGQELLFAPGAARYPIRP
                     SVYARAPQGTADPFALPNGAAFAYDLKLGPARSPIISALFDSAIASRAERLKTLWTAI
                     HQGEARAPNQAARDKLAQARDLAGWVPVTAAQANGLSYLQQFEPQGREPSPAAAAIVK
                     TWSAQIDEKQTQALRLAQDALGASTP"
BASE COUNT         1203 a         1788 c         1869 g         1187 t
ORIGIN      
        1 ctttttcacc gcgacggtga atccgaaaac cggttcgttc tcttcctggc gcagcgcgca
       61 ctccacgatg tcgacgaatt cgagacccgc acgggcaatt tcggcccgca aataggcttc
      121 gctgtgccgg aagcggcgca ccttggtctg ctcccacccc gcgccggttt tggcttcgct
      181 ggcgaaaaga tagaagccgc ccggcgcgag gcatttggcc acgccggaga ataccggtgc
      241 caggtcaccg aaatacgtca tcgtgtccgc ggagacgata aggtcgtaat tcatgccggg
      301 ttcggccaac accgtttcaa ggtcgcccag gatgagatcg cgatagatgc cccgcgcccg
      361 ggctgcttcc accatgcgag gagagaggtc gatgccgtcg atccatccgc cggacgccaa
      421 atcctggaag gcaagcccaa cgaggccggt gccgcagccc agatcgagaa tgcgccagga
      481 tgggcagaga cgcggcaaca cccgcatcgc catcgcccga acatgcaaat gggcgcggta
      541 ttgcagtttc tccagcatcg tgtggtcgta atgggtggag aattcatcga acagcccgcg
      601 catatatttg gcagggctgc gggcattgcg ccccagggtg aacagcgcaa aatcgcgatt
      661 ttccacggct tcaggaaatt cgggcccgat cgcgagcgcg cggtcgtagc tcgcgaccgc
      721 ctcttcgaag cgtcccatcc tggccaatgt gttgccgcga ttgttccagc cgatggcgag
      781 ttcggattcg atttcaagaa cggcatcgaa ccccgccaaa gccatatcga gtttgccggt
      841 ttcgagcagc agagtggcgc gattgcaaag agcctccaca aagccggggt gaatcgacaa
      901 agcgcggtca aagctggcca acgcatcctg gactcgcccc atgccggcca gcagactgcc
      961 gcggctgttc cagcgcgccg catcgtttgg gtcgcgcgcg agccctgttt cgacggttgc
     1021 gagccgattg cgcgattcgg tcaattccgc cagcaccgcg gcgcgagccg actgggcgtc
     1081 cgcaaaatcc ggttcgatcg caagcgccga atcgaaacag gtcagagcgt cttccccacg
     1141 ccccagatgc atcagcatca gtccgcgcgc gcgccagcct tccgcgaagc ccggattgcg
     1201 cgccagcgct tcgccgacgg tccgctcccc ctcgttaagc cgtccggtct gcgcgtgcgc
     1261 catgcccagg ccatagaggg cctcgaaatg ctttgggtca tcgtgcaaaa tccgctgata
     1321 gaggcgcgcg gcatccagga gccgtcccgc ctcatgcagt ctcctcgcgt cttgcatccg
     1381 aggatcggcc atgattcctt aattctaaga ccggtaacgc aggtttataa agaagttgcg
     1441 tccctccgaa gggaagcccg cggtgagctg gtaattctcg tcgaacaggt ttcgcgcccc
     1501 gatctggaaa tcgatcttct cggtaaaggc gtaatccgcc tccaaattca gcaggacgaa
     1561 cgagcccgtc ttgaggaagg aattggcatt gccgctgagg ttcgaccagc gatcgctcgc
     1621 cgcctcgatg ttcggcgtga tgctgatctg ctgcgtcaca ttccaggtca cataggcgaa
     1681 cacctggctg tagggcaggc cggtgaggtg gaaattcgtg ggcagaggcg gattgatggg
     1741 attctgcgca tcgatattgc ggttgatata ggtgtagcga acgcccgccg tcagcgtttc
     1801 ggttatggtc gtgtcggcgg aaaattcaaa gccgtaatat tttccgtcgc cgacattctg
     1861 ggtttggctg gtgacggtgt tggtgccgga aagtccgccg gcaccgctgc aatctttcgc
     1921 cgtggtcgaa gtggtgtcgc agaaattgat cggcacgttt tccagcgcgt tggtcacgtc
     1981 gctatagaaa accgctgcgt tcagccgcgt gccgaaaaac gtgtcgccgc cgccgatctc
     2041 gtaattgatg gcgcgttcgg ccttgagatt gggattggaa agcgtgctgc cgaagcgcgt
     2101 gctgaagcgt tcgaatatgg tcggaaagcg cgagcggtcc gaaacattgg catagacgtg
     2161 ccctgtatcg tcgtaattgt agatcaaggc cgcctgggcg ttgtccgcat ggccgtccgc
     2221 cagcgggaaa ttgacgaaac tgccggacgt ctttgcggtc gggtcgttgt agtcctgggc
     2281 gacgatgaga tgccgccagt catagctcgc gccggccacg aaatcgatct ggtcggtgac
     2341 atgcaggcgg ttctccaccg cggcggagta ggtgtcttcg aggtcctgtt gatggggctc
     2401 ggtgaacttc ggcacgaaga tcgtctgcca ttcgacatga ttgtcgcggc ggtagaacag
     2461 cgcccccttc aaaatatcac ggccgccgaa atcatttccg gcttcgacac tgccgccata
     2521 ggcgtaatcc gaatagaagg attgaaacgc cttcgatgtg gtttgcgaat tatagttcgc
     2581 gttgtcatag gaatagaggc cgttgcggaa gctgttgtaa tagaccttgg tcttcacata
     2641 ggcggagtcg ccgaccttgg tattcgagag gaaatagagg ctctgcacgt cccaataggg
     2701 ccaactccaa tccttctgtg ccgatacagg gtcggacacg gataaagtgg cgtccttcct
     2761 gccccattgg ccggaataac tcagggaata ttcgtcggtg gcattgggtg tcgatccgat
     2821 cttgaggttc atattgtaat tacgcgcgtc ggaatgatcg cggtcgccgc catcctcagt
     2881 cgccgtcggc gtgaacttgt ccggcagccg gaattggtcg gtcgtggtaa ccccgccgct
     2941 cgcctgccag taatatttgt ctgtggcgcc gccgactaga cctgaggtgt tgtagccgtt
     3001 gtaatcgcca ttgccgccaa gctgaatgcc ggcgcgtgct tcgtattcga acggctgggt
     3061 gggcttacgg gtgacaagat tgagcgctcc accgatggca cccggtcctg acagcacgga
     3121 cacatagccc ttctcgacct gaatctgcgc aaggttcgcg gtattgaaga acccgagatc
     3181 gagacgattg tccgccggca gatagacgcg gatgccgtcg acaaacagcg gcgattgaaa
     3241 acggtcgaag ccgcgaatga aaaacagctt ttcgttgcgc gggccacctg tcgttcccga
     3301 cgcaacgccg gtcacgagat cgagcgcgcg atccactgtg agggcgttga acttgaacgc
     3361 ctcctccgcg gacaccgtgc tctcgctgat ggttgccgcc atcggcgatg cggttacctt
     3421 gacctgttca atctggccga gattgaaaat gctgttatcc ggaacagaac ccgctgcatt
     3481 tgccgcgacc atgggattct gctgggcaag cgcggcgctc gataccagga gcgcagcaaa
     3541 cgaaacggaa ttcctcagat atgtacgaaa cattagtccc cccaaaggca gttttcaggt
     3601 cgatgatcag agcgtttgca gaaattcgac gatggccttt cgatcggtcg cggaaagcga
     3661 ggcaaaacgg tctcgcgcgt gttgcgcttc gccgccgtgc cacagaatgg cttccgtgac
     3721 atcgcgcgcg cgaccgtcgt gcaagaaggc cggatgcgtg acgaccgtgc ttgcgaggcc
     3781 aaagccccat agaggcgtgg ttcgccaatc ccggccggac gcgtcgaaat ccggacgccc
     3841 gtcggaaaga tcttctccca tgtcgtgcaa caaaagatcc gtgtagggat ggatcgtctt
     3901 gtgggcggcc tgaggaaatt gcggactgtc gcctgtcgtc agcgacggca cgtggcaggc
     3961 ggcgcaacct atgttctgaa ataaggcttg gccgtctttg gcggccgcgc cggcgggctt
     4021 gggagccggc ggcgccagag cctcgatgga aaacacaagc gtggcgaaat cggcatcgga
     4081 aatttccgga tccccgccct tggccgcgcc cgcgcacgtc ttttccgcaa ccgtgcagtt
     4141 ctgctcccgg aaaagatgcg acgtgatgcc catgtcgccg atcagggcgg cggccacctg
     4201 ttggcccaaa ttcggctgat tgtccttgag accaaagcgg ccgatcgcga tgcgtcccga
     4261 agccacgtcc caaacgcgat tgagccggcc gcgcacgccg tcgtgattcg catcgctggg
     4321 atcggcaagt cccttcagct ccgcttccgg aaccgcttcc aggagtccca gcccgaagat
     4381 ggcgggagcc atgcggaccg atgtcatcgt gccttgcgcg aatggaccgt aatggagatt
     4441 cttgaagatc agctccggac ggcgaaggga tatgcgcgtg ccgtcgtcaa gtgtttgttc
     4501 cgtttcatgc cagccgatgg tggcatcccc ttcgctggga acgccctgaa tgccgatggg
     4561 ctggagctga tcgccgtaaa ccggttccgg cgccggcgcg cccatggctg ctttccccgg
     4621 cacgctcaaa cgcaccaaac tgctgcgcag gacatcgttt tcgccggcgg acgtttcgcc
     4681 tcgcccattg ccgatatgac atccggcaca tgagagtcga ttggacaacg gacccaatcc
     4741 gtcgaaacgc ggctgttcgt cagacggcgc gacgagccag ggttgccgga aaatggatgc
     4801 accggcgcga aaccgttcca ccccggcaga atcgagaaac ggcatgggct gctggaatgc
     4861 ggcgcggcca cgattttcca ccgtcacatc cgtatcggcg gccgcgttcg ccaaaccgcc
     4921 cgcagcaaga cagaaaattc ccagcgtcag cacgtttcgt cgaatcacgg cgtgcttgcg
     4981 ccaagcgcgt cctgagcaag gcgaagcgcc tgcgtttgct tttcgtcgat ttgtgccgac
     5041 cacgttttca cgatcgcggc ggcggccggg cttggctccc gcccttgagg ttcgaactgc
     5101 tgcagatagg aaaggccgtt cgcctgggcg gcggtcactg gaacccagcc ggcaagatcg
     5161 cgcgcctggg caagcttgtc gcgtgccgcc tgatttgggg cccttgcttc accctggtgg
     5221 atggcggtcc acaatgtctt caggcgttcg gcacgcgatg caatggcgct atcgaacaaa
     5281 gccgagatga tgggagaccg tgccgggccg agtttgagat cataggcgaa cgcggcgcca
     5341 ttgggcagcg cgaacggatc ggccgtccct tgcggcgcgc gcgcatagac cgaagggcga
     5401 atcggataac gcgccgcgcc gggtgcaaag agcaattctt ggccggagtc cgagacgagg
     5461 aatgaagcga acgcgcgcgc agcctgcgca tgagggctgg acgcgacgac cgcggcatag
     5521 gcgggaagga acgcggtctg cgccggatag acaagcgata cggatttgcc gtcggagatc
     5581 gcgttgcgta tcaggaaatc gatggcgatc gccgcagccg ccttgccgtc ggcgacgtca
     5641 tcggcgatct ctccgccctt ggccgtaagc gtcgcatttg cggccatttc cgaaagcaga
     5701 gcccaacccc tatcccagcc tttggcttgc aggatgacgt cgtacaattg gggcgagaag
     5761 ccgatgcgcg aggcgaccgg catgacgaga agcccggaat attcggggcg cgcgagatcg
     5821 tcccaggttt ttggcgatgc aatccctttg gtcttcaaaa cggcgggatt cgtgacgatc
     5881 ccatagcccg caacctcgaa ggcctcgaaa tatccgttcg aatccgaaat ggcttccgcg
     5941 ccgatatggc ccggcacctg actgcgatcg acctggaatt tttcaaatgt cccggattgc
     6001 gccaatgccg ggaaattgaa aagcgcaggc gcccaataaa catcgat
//
LOCUS       DASYEG010000313         6509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3023005, whole genome shotgun sequence.
ACCESSION   DASYEG010000313 DASYEG010000000
VERSION     DASYEG010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6509
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3023005"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..823)
                     /locus_tag="VGM72_13320"
     CDS             complement(122..823)
                     /locus_tag="VGM72_13320"
                     /inference="COORDINATES: protein motif:HMM:NF005458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEY4266294.1"
                     /translation="MKSLIAIRHVHFEDLGIFAGVFAQAGYDCRYYDAGVDDLAAIDP
                     RKCDMLVVLGAPIGAYEDDKYPFLSHELHLLRDRLAAKLPTLGICLGAQLMARALGAS
                     VHPGTAKEIGWQPLALTAEGRAGPLMHLENIPVLHWHGDTFSLPQGAVCLASTPITKN
                     QAFSFGTNTLALQFHPEAGTEGFERWLIGHALEIATAAGVSVEILRRDTARYAAHCGE
                     QGRHVVKDWLASLRP"
     gene            1059..3392
                     /locus_tag="VGM72_13325"
     CDS             1059..3392
                     /locus_tag="VGM72_13325"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY4266295.1"
                     /translation="MKFKAVGQPIRRNEDERLITGHGRFTDDFSLPGQVWTAMVRSPH
                     PHAAIVGIDSSAALEMEGVLGVFTGADLAADGLGEIPHNPVPSTKYDMKLTGRGGTPV
                     YIGDHFLLPTDRVRHVGEALAMVVAETRQQAQNAAEAVMVDYDLLPWVADTAAAAKPD
                     APRVWDALPDNVLVDSSFGDQENTAAAFARATHAVSMHYHLGRVTGAPLEPRAALGHY
                     DAASGRYTLYAGSGGAVRQKHEMAAVLGVTPDKLRVISFDVGGNFGTRNRAFVEFGLV
                     IWASKKIGRPVKYRADRTECFLSDYQGRDLVVEISLAIDAEGKFLALRSDNLSNVGSH
                     CVSLSPLSKGSGLITGSYDIPYATLRSRAVYSNTMSTQAYRSSGRPEVNFALERLIDK
                     AARELGFDRLELRRKNLIKPSQMPYTNAVGSQYDSGEYEKNMDRLLEIGDFAGAQTRK
                     AQAKSRGKLYGIGSTNYVESSIGTPKERADLIVREGGIEIVIGTQPAGQGHQTSFAQV
                     TADLLGVPFETVTITLGDTDIVSAGGGTHSGRSMRHASAVISMAVTDMIVKAKTILAE
                     SLGVAPSDVQFEDGMFRSPATNRTVDWFELARLAVTLSPDYADGVKVRRDNEMHTPVF
                     PNGACMCEIEIDPETGGLEIMRYATVDDVGRCINPMIVHGQTHGGIAQGVGQALWEGC
                     VIDPDSGQPLAAAFTDYGMPRFDNMPSFITEIAEVLSPTNPLGIKAGGEGGTTPAPAV
                     IMSAIDDALRDYGPPGISMPITPVKVWTAIHKGAGAI"
     gene            3392..3922
                     /locus_tag="VGM72_13330"
     CDS             3392..3922
                     /locus_tag="VGM72_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY4266296.1"
                     /translation="MSAIAMNVNGSNVRKEVGDSTLLVEFLREHLGLTGTHVGCDTTQ
                     CGCCVVHLDGVSVKSCTVLAAQAAGRAVTTIEGLARNGELHPVQKAFREHHGLQCGFC
                     TPGMIMAAVELIATYPGRLDEHVVREEMEGNLCRCTGYHNIVRAILAAASQMLGPDRV
                     VQPPADHMPAPRKSEG"
     gene            3927..4742
                     /locus_tag="VGM72_13335"
     CDS             3927..4742
                     /locus_tag="VGM72_13335"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY4266297.1"
                     /translation="MYPLTYHRPKTLAEAKEMFGAGADATFLSGGHTLLPTMKQHLAA
                     PSDLIDVTAIAELHGIAADGKLLKIGAAMTHAEVSESPIVRLAIPVLAGLAGSIGDRH
                     VRHRGTIGGSIANNDPAADYPSAVLALNAMVVTDRRRVAADDFFTGLYQTAREPGEIV
                     TRIEFPIPTQACYAKFRSPASRYSIVGVFVARTDKAVRVAVTGAGASGVFRPAAFEQA
                     LAADFRSEAIAHCSVDPALLLSDLNGTAEYRAHLIGVLASRAVAKPGQIQSFK"
     gene            complement(4760..5524)
                     /locus_tag="VGM72_13340"
     CDS             complement(4760..5524)
                     /locus_tag="VGM72_13340"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY4266298.1"
                     /translation="MVEPIAKLAPLQHQAAPLRGKIIGALRNAIETGMLQPGARLVER
                     DLCGQLNVSRTSLREALRELQADGVLFQSGARGLAVSRITREEAANAYRIRAVLEALA
                     VEQFIEHASDQNIRQIAKDADALKAAYRSGDVSRMLLSRRAFYDRICSVAGNALAFDL
                     INRLVLRTSSLRGRSLSRKQRQQQSQGEIDAIVKAIRKRDTVTAKRAAIAHVESAAAS
                     ALESHNPAKREGIPEAAKTRPKPAKPARPTRSKGRT"
     gene            5727..6290
                     /locus_tag="VGM72_13345"
     CDS             5727..6290
                     /locus_tag="VGM72_13345"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEY4266299.1"
                     /translation="MNMTGERFIAAPRMAVWASLNDPEVLKACIPGCESLEKISETQM
                     KAVAGIKLGPVSAKFQGQVTLSDLDPPNGYKISGEGKGGPAGFAKGGAEVRLLDQDAG
                     TLLTYSVDAQVGGRIAQVGARLIDATAKSLAEQFFKKFAAVVEKPQTAAEPMRETTRE
                     ASRNPGLFARIWAAIVRLFSGPGKTKD"
BASE COUNT         1255 a         1953 c         2058 g         1243 t
ORIGIN      
        1 agggcggcgt tgtgagcatc gccaatggtc tcaagagcgg cgagaaagtc gcgctcaatg
       61 ttcccgacga tgtgtccgac ggcagccaca tccagcccgt ccccgcggtg cagtagctga
      121 tctaaggacg cagcgacgcc agccaatcct tcaccacatg ccggccttgc tcgccgcaat
      181 gcgccgcata acgcgcggta tcgcgccgaa ggatttcgac ggaaaccccc gcggcggtcg
      241 cgatctcgag cgcatgaccg atgagccagc gctcgaagcc ttccgtcccg gcctcggggt
      301 ggaactgaag cgccagcgta ttcgtcccga atgagaaagc ctggttcttg gtgatgggtg
      361 tcgaagccag gcataccgca ccctgcggca gggagaatgt gtcgccatgc caatgaagca
      421 ccggaatgtt ttcaagatgc attagcggtc ccgcgcgtcc ctccgcggtc aatgcaaggg
      481 gctgccagcc gatctctttt gccgtgcccg gatggaccga tgcgccgagc gcgcgggcca
      541 tcaattgagc gcctaggcaa atgcccaagg tcggaagctt cgcggccaga cggtcgcgga
      601 gcaggtgcag ttcgtggcta agaaaaggat atttatcgtc ttcataggct ccgatgggcg
      661 caccgaggac aaccagcatg tcacatttgc gtggatcgat ggcggcaaga tcgtccacgc
      721 ctgcgtcgta atagcggcag tcatacccgg cttgtgcaaa tacgccggcg aatattccca
      781 ggtcctcgaa atgcacatga cggatggcga tgagggattt catggaaaat atctctttag
      841 gtttgtgctc gggccatcaa gaattccata cagtaaggct tgtaacccgg tcctgtccgc
      901 atcaaattcc ggtaatagga tggcgcttga cgcggagtaa acggctggaa ttggcgtaat
      961 ttttgacggc ttgcgaattt tggtcgacca taatacaaaa gaccaaatac gagacgcatc
     1021 gtccaaaaac ccggaacttg catggcatgg aaccgaagtt gaaattcaaa gctgtcgggc
     1081 aaccgatccg ccgcaacgag gatgaacggc tcatcaccgg ccatgggcgc ttcaccgatg
     1141 atttcagcct gcccggtcag gtctggaccg caatggtgcg ttcgcctcac ccgcacgcgg
     1201 ccatcgtggg gatcgattcc tcggctgctc ttgagatgga aggcgtgctt ggcgtcttca
     1261 caggtgcgga cctcgcggcc gatggcctgg gtgagatccc gcacaatccg gtcccgtcca
     1321 ccaaatatga catgaagctc acggggcgcg gcggtacgcc ggtctatatc ggcgaccact
     1381 tcctgttgcc gacggatcgc gtgcgtcatg tcggcgaagc gcttgccatg gtggtggccg
     1441 aaacgcgcca gcaggcacag aacgccgcgg aagcggtgat ggtggattat gatctgctgc
     1501 cctgggtcgc ggataccgcc gcggcggcaa agcccgatgc gccgcgcgta tgggatgcct
     1561 tgcccgacaa tgtcctggtc gacagcagct ttggcgacca ggagaacacc gccgcggcat
     1621 tcgcacgcgc cacccatgcg gtcagcatgc attatcatct ggggcgtgtg accggcgcgc
     1681 cgctggaacc gcgcgcggcg ctgggtcatt acgatgcggc gtccggccgt tatacgcttt
     1741 atgccggcag cggcggcgcg gtgcgtcaga agcacgaaat ggcggccgtg cttggcgtaa
     1801 cgccggacaa attgcgcgtt atctcgtttg atgtcggcgg caatttcggc acccgtaacc
     1861 gcgcgttcgt ggaattcgga ctggtgatct gggcttcgaa gaaaatcggc cgtcccgtaa
     1921 aataccgcgc cgaccgtacc gaatgtttcc ttagcgatta tcagggccgc gatctggtgg
     1981 tggagatatc gttggcgatc gacgcggagg gaaaattcct ggctctgcgg tcggacaatt
     2041 tgtcgaatgt cggctcgcac tgcgtgtcgc tgtcgccgct gagcaaggga tcggggctga
     2101 ttaccggctc ttacgacatc ccttacgcga cgctgcgttc gcgggcggtc tattcgaata
     2161 cgatgtcgac ccaggcctat cgcagctcgg gacggcccga agtgaacttc gccttggaac
     2221 ggctgatcga caaggcggcc cgcgaactcg ggttcgaccg tttggagctg cgccggaaaa
     2281 atctcataaa gccgtcgcag atgccgtaca ccaatgctgt cggatcgcaa tatgacagcg
     2341 gggaatatga gaagaacatg gaccgcctgc tggagatcgg cgatttcgca ggcgcccaaa
     2401 cccgtaaagc gcaggccaag tcgcgcggca aattatacgg catcggttcc accaattacg
     2461 tcgagtcctc gatcgggacg cccaaggaac gcgccgacct catcgtgcgc gagggcggga
     2521 tagaaatcgt cattggaacg caacccgcag gtcagggcca tcagacgagc ttcgcccaag
     2581 tcacggccga tctgctcggt gtcccgtttg agacggtgac gatcacgctt ggcgataccg
     2641 acatcgtgtc cgccggaggc ggcacccatt ccggacgatc gatgcgtcat gccagcgccg
     2701 tcatctcgat ggccgtcacc gatatgatcg tcaaggcaaa aacgattttg gcggagagtc
     2761 tcggcgtcgc gccgtccgat gtgcagttcg aagacgggat gttccgtagc cccgccacca
     2821 atcgcacagt cgattggttc gagttggcgc ggctggccgt gacgctttcg cccgattacg
     2881 ccgatggggt gaaggtacga cgcgacaatg aaatgcacac gccggttttt cccaatggcg
     2941 cgtgcatgtg cgagatcgag atcgatccag aaaccggcgg gttggagatc atgcgctatg
     3001 ccacggtcga cgatgtcgga cgctgcatca atccgatgat cgtgcacggc cagacccatg
     3061 gcggtatcgc gcagggcgtg gggcaggcgc tgtgggaggg atgcgtgatc gatcccgact
     3121 ccggtcagcc gctggccgct gcgttcaccg attacgggat gccgcgtttc gacaacatgc
     3181 cgtccttcat caccgaaatc gcagaggtgc tttcgcccac caatccgttg gggattaagg
     3241 ccggaggcga aggcggcacc accccagcac ccgccgtgat catgagcgcg atcgacgatg
     3301 cgctccgcga ttacggtccg cccggtatct ccatgcccat cacaccggtg aaggtgtgga
     3361 ccgccatcca taagggcgcg ggagccatct gatgtccgcc atcgcaatga acgtgaacgg
     3421 ttcgaatgtc cgcaaggaag ttggcgattc gacgctgctg gtggaattcc tgcgcgaaca
     3481 cctgggcctg accggcacgc acgtcggctg cgacaccacg cagtgcggct gctgcgtggt
     3541 gcatctcgac ggtgtctcgg tgaaatcctg cacggtgctg gcggcacagg ccgcgggccg
     3601 ggccgtgacc acgatcgaag gactggccag gaacggcgaa ctccatcctg tgcagaaggc
     3661 atttcgcgag caccacggcc tgcaatgcgg cttctgcacg cccggcatga tcatggccgc
     3721 cgtcgaactg atcgccacct atcccggcag gctggacgag catgtcgtgc gcgaggagat
     3781 ggaaggcaat ctctgccgtt gcacgggcta ccacaatatc gtgcgggcca tcttggccgc
     3841 ggcatcgcag atgctcggac cggatcgcgt ggtccagccg cctgcggacc acatgcccgc
     3901 gccgcgcaaa tcggaaggat agcgcgatgt atcctttgac ctatcaccgc ccaaagactc
     3961 ttgccgaggc caaggagatg tttggcgcgg gcgccgacgc gaccttccta tcgggcgggc
     4021 ataccctgct tcccaccatg aaacagcact tggcggcgcc cagcgatctg atcgatgtga
     4081 ccgccatcgc ggagctgcac ggaatcgcag cggatgggaa attgttgaag atcggcgcgg
     4141 ccatgacgca tgccgaagtg tcggagtcgc ccattgtgcg gctggcgatc ccggtgcttg
     4201 ccggccttgc cggttctatc ggcgaccggc atgtgcgtca tcgcggaacc atcgggggtt
     4261 ccatcgcgaa caatgatccg gcggcggatt acccctctgc tgtgctggcg ctgaatgcga
     4321 tggtcgtgac ggaccggcgg cgcgtcgcgg cggatgattt ctttaccggc ctgtaccaga
     4381 ccgcgcgtga gccgggggag atcgtcaccc gcatcgaatt ccccattccc acgcaggcgt
     4441 gctacgccaa attccgcagc cctgcctcgc gctattcgat tgtgggcgtg ttcgtggcgc
     4501 ggacggataa ggccgtgcgg gttgcggtga cgggcgccgg tgcatcgggg gttttccgcc
     4561 cggctgcatt cgagcaagcg ttggctgcgg attttcgcag cgaagcgatt gcccattgca
     4621 gcgtcgatcc ggcacttctg ctcagcgatc tcaacggcac ggccgaatat cgcgcgcatc
     4681 tgatcggggt cctggcgtcg cgcgccgtcg ccaagcccgg acaaatccaa tcgttcaaat
     4741 aatgccagtc cgcgatgcgt caggtccggc ctttggagcg agtcgggcgt gcgggcttgg
     4801 ccggtttcgg ccgcgttttt gccgcctccg ggatgccttc gcgtttcgca ggattgtggg
     4861 actccaaagc cgatgccgcg gcgctctcca catgggcgat ggccgcgcgc ttggccgtga
     4921 ccgtatcgcg cttgcggatc gccttgacga tcgcatcgat ttcgccctgg ctctgctgtt
     4981 ggcgctgttt gcgggagagc gagcggccgc gcaggctgga cgttcgcagg acaagccggt
     5041 tgatgagatc gaacgccagc gcattgccgg caaccgagca gatgcggtca tagaacgcac
     5101 gccggctcaa cagcatccgt gatacgtcgc cggaacgata ggcggccttc agcgcatcgg
     5161 cgtccttcgc gatctgccga atgttttgat cgctggcgtg ctcgatgaat tgttccaccg
     5221 ccagcgcctc cagtaccgcg cggatgcgat aggcattggc ggcttcctcg cgtgtgatcc
     5281 tgctgaccgc gagtcctctt gcgccggatt ggaacagaac gccgtccgcc tgcagctccc
     5341 gcagcgcctc acgcagggat gtccggctga cattgagctg gccgcagaga tcgcgctcca
     5401 ccagacgggc acccggctgc agcatgccgg tctcgatggc gttgcgcagc gcgccgatga
     5461 tcttgccgcg caagggcgcg gcttgatgct gcagcggcgc cagtttcgct ataggctcga
     5521 ccaccgcatt tcctctcgac tcgacgtaaa gcgcaaaaga ttgcgcggta tgatgcgaca
     5581 gtagattcta tcggttggcc ggacaaggtg acgatgtatt tgtgtttgcg atgggcccat
     5641 cccgactcgc gcggccgcgt tgcgccgtgt tattgtagga cagacataca aaagaccaat
     5701 gtcggtcagc gcgaggaatc cacccgatga acatgaccgg agaacgtttt atcgctgcgc
     5761 cgcgcatggc tgtctgggca agcttgaacg atcccgaagt cctgaaagcc tgtattcccg
     5821 gctgtgagag cctggagaaa atctccgaga cccagatgaa ggccgtggcg ggaatcaagc
     5881 tcggccccgt atcggccaaa ttccaggggc aagtcacgct ttccgacctc gatccgccga
     5941 atggctacaa gatcagcggc gagggaaagg gcgggccggc cggtttcgcc aaaggcggcg
     6001 cggaggtgcg tctccttgat caggatgcgg gtacgcttct gacctacagc gtggatgcgc
     6061 aggtcggcgg cagaatcgct caggtgggcg cacgcctcat cgatgccacc gcgaaatctc
     6121 tggcggagca gttcttcaaa aaattcgctg ccgtcgtgga aaaaccgcaa accgccgcgg
     6181 agcccatgcg cgaaaccacg cgtgaggcaa gccgcaatcc tggccttttt gcccgtatat
     6241 gggccgcgat tgtgcggttg ttttccgggc cgggcaaaac caaggattag aaggcgccaa
     6301 gacgtcgttc acgcgcggtg caagaaatta ctgtctctcc aacctcttga tatgctttaa
     6361 cgctccaggc tcgcatgtgc tctgacgggg tattctgcgc gagcccatcg cgaaggtagg
     6421 accgtttgaa aatccgtctc cacagggacg acttgcctga gggcctcgat ttaggccccc
     6481 aaatcgccgt cgataccgaa accctgggg
//
LOCUS       DASYEG010000314         5913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_413126, whole genome shotgun sequence.
ACCESSION   DASYEG010000314 DASYEG010000000
VERSION     DASYEG010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413126"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VGM72_13350"
     CDS             <1..401
                     /locus_tag="VGM72_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266300.1"
                     /translation="PENLVRRQAAEWDPLLDWARTRYGANLVCGAGIVHVEQPPQTLE
                     ALESAIAQHDDFELAGLHAAATLLGSVIVALALSDGHVSAEQALAAAQLDELYQAERW
                     GQDAEMLNQFHKKTTKLTDITKYFLFLREC"
     gene            501..1142
                     /locus_tag="VGM72_13355"
     CDS             501..1142
                     /locus_tag="VGM72_13355"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY4266301.1"
                     /translation="MEAQPDRPAQQRVQGKRASIKEQNRRLILDAARRVFAESGYGGV
                     TVRDIIRATPLASGTFYNYFRSKEEIFQAMRDETALALRPILRDARRDAGTAEAFVAE
                     TFRAFFTYVVQRRGTVAVIRRSDAAPLRVDTPEVLAGFEELKADVEAAIARGLFAPVD
                     SEYLAASMLGIACELAEILQWREIPEPDEIARFATALVLGGLAALPKPVPPKS"
     gene            1150..1437
                     /locus_tag="VGM72_13360"
     CDS             1150..1437
                     /locus_tag="VGM72_13360"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEY4266302.1"
                     /translation="MSDDITTMNIRIEGQVQGCGFRDFAVHEANARKLKGWVRNRSDG
                     SLEAVASGPTKEVEAFIAVCMQGPRGARVTALNLQPVEVPESLGFIRRPSV"
     gene            complement(1415..1840)
                     /locus_tag="VGM72_13365"
     CDS             complement(1415..1840)
                     /locus_tag="VGM72_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266303.1"
                     /translation="MPRVSLWFFAVAPVYVLIGMGFGIFMGATMNFTLAPAHAHLNLI
                     GWVTMALYGTFYALAREASQKLAWTVFWLNNAAASIMFPSLAMVLIQGDSSPFLVPLI
                     VSEFLALGAMLCFAFSVWSVLMKNAGVSTSAAIRPTDAE"
     gene            complement(1915..2598)
                     /gene="lipB"
                     /locus_tag="VGM72_13370"
     CDS             complement(1915..2598)
                     /gene="lipB"
                     /locus_tag="VGM72_13370"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEY4266304.1"
                     /translation="MIVLSTALMNGTFDRIDWTFGADRVAYPDALARMEQRVDAIHRG
                     EAPEEVWLIEHPALYTAGTSARDGDLLAARFPVFKTGRGGQYTYHGPGQRVAYVMLDL
                     KRRKNDVRRFVRDLEEWLIRALAEFNVKGERRPGRIGIWIGNDDGSEDKIAAIGVRVR
                     HWVTFHGVSLNVDPELEHFSGIVPCGVRDEKFGVTSLAALGIPVSMADVDVALKRSFE
                     QIFGTVRAT"
     gene            2633..2878
                     /locus_tag="VGM72_13375"
     CDS             2633..2878
                     /locus_tag="VGM72_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HEY4266305.1"
                     /translation="MSSLSDVKVEIAVVLGRSVIPMHQLLRMGRGAVIELDSHQDDPV
                     MILANNKPVAKGEIQIDGDRIAVSISEILRAYTPCST"
     gene            2985..3497
                     /locus_tag="VGM72_13380"
     CDS             2985..3497
                     /locus_tag="VGM72_13380"
                     /inference="COORDINATES: protein motif:HMM:NF024638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4266306.1"
                     /translation="MLPIILDCSMVRAGVAGAGEGLARRLKIMKAGGVEEPVIFSGRI
                     PSQDEIAGLQILFVAGLDPSTSRSIAAAARAAGALVNVEDVPELCDFHVPAEVRRGNF
                     LLAISTAGLSPALSRAVREHLEVRFGPEWEGRLEEIAGLRQGWRAAGTAPDEVSKRTR
                     ELLAERGWLR"
     gene            3494..4249
                     /locus_tag="VGM72_13385"
     CDS             3494..4249
                     /locus_tag="VGM72_13385"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEY4266307.1"
                     /translation="MNVIPLHQADSATHALREELARLRGESAGLDTGDFLVFALTRAF
                     EGRIAVVSSFAAESVVLLHHVAEIDPATPVLFLNTGKLFGETLRYRDRLQDVLGLTDI
                     RAIGPHPDDRKTADAEGTLWSRDPDACCHFRKVVPLSRAVAGFDATITGRKRFQTATR
                     ATMNRVELVDGRFRFNPLVDWTLDELAAYTEAKGLPKHPLVLDGYPSIGCMPCTRRVA
                     AGENYRDGRWSGFAKDECGIHGGVRAGTDGDGI"
     gene            4400..4483
                     /locus_tag="VGM72_13390"
     tRNA            4400..4483
                     /locus_tag="VGM72_13390"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4433..4435,aa:Leu,seq:gag)
     gene            5710..>5913
                     /locus_tag="VGM72_13395"
     CDS             5710..>5913
                     /locus_tag="VGM72_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266308.1"
                     /translation="MRIVSLFPAVMALFLSGAASAQSFDVYTNQENFFTVNFPGTPTA
                     TQVTHKTLKGTELTAHVFTATVPP"
BASE COUNT         1125 a         1779 c         1797 g         1212 t
ORIGIN      
        1 ggccggaaaa tctggttcgg cggcaggccg cagaatggga cccgcttctg gactgggccc
       61 gcacgcgcta tggcgcgaat ctcgtttgcg gcgcgggcat cgtgcatgtc gaacagccgc
      121 cgcagaccct ggaggcactg gagagcgcca tcgcccagca tgacgatttc gaactcgcgg
      181 ggctgcatgc ggcggccaca ttgctgggct cggtgatcgt cgcactcgcg ctttcggatg
      241 ggcacgtcag tgcagagcag gccctcgcgg ccgctcaatt ggacgagctt tatcaggctg
      301 agaggtgggg acaggatgca gaaatgctaa accaatttca taagaaaacg acaaaactta
      361 ctgatatcac aaaatatttt ctatttttac gcgaatgttg acgctgtctt taacttaaac
      421 ggaacggtaa gttgaaggtc agtcatcttg cgctagaatc gcccctgcgg agccggcacg
      481 gccgtaaaga aggaccgcct ttggaagccc agcccgatcg cccggcgcaa cagcgtgtcc
      541 agggcaagcg cgcctccatc aaggagcaga atcgtcgtct gatcctcgat gccgcacgcc
      601 gtgtcttcgc cgagagcggc tatggcggcg tcacggtgcg cgacatcata cgggcgacgc
      661 cgctggcttc gggcaccttc tacaattact tccgctcgaa agaggaaatc ttccaggcca
      721 tgcgggacga aaccgcgctc gcgttgcggc cgatcctgcg tgacgcgcgg cgggacgcgg
      781 ggacggcgga ggcgttcgtg gcggaaacct tccgagcctt cttcacgtat gtggtgcaaa
      841 ggcgtgggac tgtggctgtg atcaggcgta gtgatgcggc gcccttgcgt gttgatacgc
      901 cggaggtgct tgccggcttc gaggaactga aagccgatgt agaggccgcc atcgcgcgcg
      961 gtcttttcgc acccgtggat tcggaatatc tggcggcgtc catgctgggg attgcttgcg
     1021 aactggctga gatcctgcag tggcgggaaa taccggaacc cgatgagatc gcacgttttg
     1081 ccaccgcctt ggtcttgggc gggctcgcag cccttcctaa accggtccct ccgaaatcct
     1141 aggaatccca tgagcgacga catcaccacc atgaacatcc gcatcgaggg gcaggtgcag
     1201 gggtgcggct ttcgcgactt cgcggtgcac gaagccaacg cgcgcaagct gaaaggctgg
     1261 gtgcgcaacc gctccgacgg atcgctcgaa gcggtggcgt cgggccccac caaggaggtg
     1321 gaggccttca tcgccgtctg catgcaaggg ccgcgtggcg cgcgcgtgac cgctctcaat
     1381 ctccagcccg tggaagtgcc cgaatcgctc ggctttattc ggcgtccgtc ggtctgatcg
     1441 cggccgaggt ggagacgccc gcgtttttca tcagcacgct ccacaccgag aacgcgaaac
     1501 acagcatggc gccgagcgcg aggaattcgc tgacgatcag cggcacaagg aatggtgagc
     1561 tgtcgccctg tatcagcacc atggcgagag aggggaacat gatcgaggcc gcggcattgt
     1621 tcagccagaa caccgtccag gcgagcttct gcgaagcctc gcgggcgagc gcgtagaatg
     1681 ttccatagag cgccatcgtg acccagccga tcaagttcag atgggcgtgg gcgggggcga
     1741 gcgtgaagtt catggtggcg cccatgaaga tgccgaaccc catcccaatc agcacataga
     1801 ccggcgcgac ggcaaagaac caaagcgaga cacgcggcat ggtagccccc tgcattttgc
     1861 gcagggactg tgatcaatcc gcaggttcct gccaatgctt ttttcgcagg ccgcctatgt
     1921 ggctctgacc gtgccgaata tctgttcgaa gctgcgcttc agcgcgacgt cgacatcggc
     1981 catgctgacc ggaatgccca gggcggcaag gctcgtgacg ccgaatttct catcgcgcac
     2041 gccgcagggc acgattccgg aaaaatgctc aagctccggg tctacattca ggcttacgcc
     2101 gtggaatgtt acccagtgcc ggacacgaac gccgatggcg gcgatcttgt cttcgctgcc
     2161 atcgtcattg ccgatccata ttccaattcg tccgggtctt cgttcgcctt tgacgttgaa
     2221 ctcggcgagc gcgcggatca gccattcttc cagatcgcgc acgaaacgcc gcacatcgtt
     2281 tttgcggcgt ttcaaatcca gcatgacata ggctacgcgt tggcccggcc catgataggt
     2341 gtattgtccg ccgcgtccgg ttttgaacac cggaaaacgc gccgccagca aatcgccgtc
     2401 ccgggcgctg gtgccggcgg tgtagagcgc gggatgttcg atgagccaga cttcctcggg
     2461 cgcttcgccc cggtggatcg catcgacacg ctgttccatg cgcgcgagcg catccggata
     2521 ggcgacacga tccgcgccga aggtccaatc gatcctatcg aatgtgccgt tcattaacgc
     2581 ggtagaaagc acaatcattc atggttggca tcaggttctt tcccaggtgg ccatgtcttc
     2641 gctcagtgac gtcaaagtcg aaatcgccgt tgttctcggc cgttcggtca ttcccatgca
     2701 tcaacttttg cgcatgggcc gcggcgcggt catcgagctg gattcccatc aggacgaccc
     2761 cgtcatgatc ttggccaata acaagccggt ggccaaaggc gagatccaga tcgacggcga
     2821 cagaatcgcg gtctccatca gcgaaatttt gcgggcctac acaccatgtt caacgtgaaa
     2881 taaagtgtaa attaaattat tccccataca aatcttgcaa aaccttgggg cagagtttag
     2941 ctctacctga aatcgccttt tcgcgatttc ggaaaaacat atccatgttg cccataatcc
     3001 tggactgttc gatggtccgc gccggtgtcg ccggcgccgg cgaagggctt gcacggcggt
     3061 tgaaaatcat gaaggcgggc ggtgtggagg agccggtgat tttttccggc cgaatcccca
     3121 gccaggacga gatcgccggt ctccagattc ttttcgtcgc cggtctcgat ccatccacct
     3181 cgcgcagcat tgccgctgcg gcacgcgccg cgggcgcgct cgtcaatgtc gaggacgtac
     3241 cggaattgtg cgatttccac gttccggctg aagtccgccg cggcaatttt cttctggcca
     3301 tatccaccgc ggggctgtcg cccgcgcttt cgcgcgcggt tcgtgaacat ctcgaggttc
     3361 gattcgggcc ggaatgggaa ggcaggcttg aggagatcgc cggtcttcgc cagggctggc
     3421 gggcggcggg gactgccccg gacgaagtat cgaagcgcac gcgcgaactt ctcgccgaac
     3481 ggggctggct gaggtgaacg tcattccgct tcatcaggcc gattctgcca cgcatgccct
     3541 gcgcgaggaa ctcgcccggc ttcgcggtga gagcgcgggc ttggacaccg gggattttct
     3601 ggtcttcgcg ctgacccggg cgtttgaggg ccgcatcgcc gtggtttcgt ccttcgccgc
     3661 cgagtccgtg gtgctgctgc atcacgtcgc cgaaatcgat ccggcgactc cggtgctgtt
     3721 cttgaacacc ggaaagctgt tcggcgaaac gctgcgctac cgcgaccgac ttcaggatgt
     3781 gctcggcctg accgacatcc gcgccattgg accgcatccc gacgaccgca agacggccga
     3841 tgccgaaggc acgctgtgga gccgcgatcc cgatgcctgc tgtcattttc gcaaggtggt
     3901 gccgttgagc cgtgcggtgg cgggattcga tgcgaccatt accgggcgca agcgcttcca
     3961 gaccgcgacg cgcgcaacca tgaaccgggt ggaactggtc gatggccgct tccgcttcaa
     4021 tccgctggtc gattggacgc tggacgagct tgccgcctac accgaggcga agggtctgcc
     4081 caaacatcct ttggtgctgg acggctatcc gtcgatcggc tgcatgccgt gtacgcggcg
     4141 cgtggcggcg ggggagaact atcgcgacgg ccgctggtcc gggttcgcca aggacgaatg
     4201 cggcattcat ggcggtgtgc gggccggcac cgacggcgac ggcatctaaa actccccctt
     4261 gcggtcttga gcctttggga gagggccgcg gtcggcgggg gccatttgcg gaccggggag
     4321 ggggatttcc atcctcgatg cgttggtgct tcccaacggg agcggggttt gttacatccg
     4381 gccgccctct catacacgtg cggtcgtggc ggaacggtag acgcgctagc ttgaggtgct
     4441 agtagggcaa cctgtggaag ttcgagtctt ctcgaccgca ccacctaaaa ataagcgatt
     4501 gccggtgcgg cctgcggcgg cgcgcgcatc cgggagagtt tcaggtttgg ccaattcggc
     4561 cagggaagcg atcgtagcgt gtgcctggct ttaggctgaa atcacacgaa atcgtaccga
     4621 aattttactc gactttgccg cgttgctgaa tagaaaattc ccaccgtctt ctgatgtttg
     4681 gacgggtgcc atggccgatt atcgtctcta cgttctggat tcttccggtc acattacgcg
     4741 ccgtgaggac ttccaatttc ccgatgatga ccaagcaatc gcacacgcaa tacagttcgt
     4801 gaacggtaaa gctctggagt tatggtcggg cacgcgggtt gtcacgcgca tagaacccaa
     4861 cagcgcggaa atgtccgctg gcgtaacgcc gcctccgcac gactccgcca atagcccttg
     4921 atgccggttc cttagacttt tggagtgacg cgtacagcac cgccgtcatc cgcggccatc
     4981 agtgttgcgg tagcaaaaga gcgcgcagat ctttaatcca tgccacggcg atgatgaagg
     5041 catggctcgc agcgcttcac ctcaccgctt cgccttcgaa aggcctgctt tctttcgacc
     5101 gccgccacca ccctgcctct gcactcgctg acgcgtctcc cgacgggatg atttcccgac
     5161 gctatcgtcg cactggcttg tcagctcccg gcgtaggaac gctgatctgc acgctagcac
     5221 cacaccgaca atcatacgtt tgtagctcgt gttgcggacc gcgcactgga tgcggctccc
     5281 tacgggtgag cttcatcaat cgcgcacaaa caatacattt cggccgcttg tgcgcgccaa
     5341 attgagtggc cgtctgtgcc tgtgagttca ccgcttcccc ttagccggcg ctttcgcgct
     5401 tggtcccgct caatcgccac ccgacttctg cgagcgcaaa tgctagtcta tctcgccgga
     5461 ggcgaagcac gccggttgca ttttcgtgag aaccgccgcg ctcattcctc ggcctccagg
     5521 tcagaggcgc ctttggggca agagcagaat gtctcccggc catccgccct aatcgcttgc
     5581 agatgatcag ctattctagg ccttaaccgc atgccgtcct aatggttaac gcgtacggaa
     5641 tggtacgcat ctgcattgga agagcattta tgccaatatg ggtctggata atgtttaggg
     5701 gagattcggt tgcgcatcgt cagtcttttt ccggccgtga tggctttgtt cctctccggc
     5761 gcagccagcg cacaatcctt cgacgtctac accaaccagg agaatttctt cacggtaaat
     5821 tttccgggca cccctacggc aacgcaggtg acgcataaga cgctgaaagg cacggaattg
     5881 accgcgcatg tgttcaccgc gacggtcccg ccc
//
LOCUS       DASYEG010000315         5202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4904779, whole genome shotgun sequence.
ACCESSION   DASYEG010000315 DASYEG010000000
VERSION     DASYEG010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4904779"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="VGM72_13400"
     CDS             <1..1007
                     /locus_tag="VGM72_13400"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEY4266309.1"
                     /translation="NFLAMQQAIVDSGLEEKDIVNERTGLIMGSGGPSTRAIVAAADT
                     ARVEPVKGPKRVGPFVVPKAMSSTCSAVLSTWFKIKGLSYSISSACATSAHCIGNAAE
                     LIQWGKQDIIFAGGGEELDWSLSVLFDAMGAMSSHFNAAPERASRAYDKNRDGFVIAG
                     GGGTLVLEELEHAKARGAKIYAELVGYGATSDGHDMVAPSGEGAVRCMRQALATVKAP
                     LDYVNAHGTSTPVGDAVEIAAMREVFEKNMPPVNSTKSLTGHSLGAAGVHEAIYSLLQ
                     MQSGFIAESANIEEIDPEVADAPIVRKRIDNAKLQTVMSNSFGFGGTNASLVFQRYDA
                     "
     gene            1064..1912
                     /gene="fabI"
                     /locus_tag="VGM72_13405"
     CDS             1064..1912
                     /gene="fabI"
                     /locus_tag="VGM72_13405"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005250713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEY4266310.1"
                     /translation="MAEAYAAKPGTLMAGKRGLIMGVANDHSIAWGIARTLAAHGAEL
                     AFTYQGDAQGKRVRPLAESIGARLVIACDVGNDAEVETGFATLQAEWGGLDFLVHAIA
                     YSDKNELKGRYVETSRQNFISTLNISCYSFTDVARRAALLMKNGGALVTLTYGGASRV
                     MPNYNVMGVAKAALEASVRYLAADLGKQDIRVNAISAGPMRTLAGSAVGDARMVFKWN
                     KAHSPLKKNIELDHVGGAALYLLSDLSAGVTGEIHFVDAGYNTVGMPPTADLKGWIAP
                     ENGEPE"
     gene            1940..2680
                     /locus_tag="VGM72_13410"
     CDS             1940..2680
                     /locus_tag="VGM72_13410"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY4266311.1"
                     /translation="MKTPAQRKSRFGPPINVEEEMRTGAIAYWQGDYYQSRADRGGVS
                     LLDYIHALFGLLMQKRPKTVLMIGCAGGSLATMLARIGVAVTAIDVNPQAFAIARRYF
                     GLPDSVECRVADGLDYLVSTRRMFDAVALDAFHGGRVDEDLISDVFFARTAKRLRKNG
                     AVFANIHVLDDSDGAADRAAAHMAAAFSDVRILDRRRAKPRNAIVMAGAVRGLKKPAL
                     LMPPAIETGKITRVLDAMQFRPPLRSQR"
     gene            2844..3623
                     /locus_tag="VGM72_13415"
     CDS             2844..3623
                     /locus_tag="VGM72_13415"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY4266312.1"
                     /translation="MRTLVAQAILLFALSLAPACALSSEPYLKPGQVDALVLIAPPPA
                     GEIQARDLADVLRAQAARTPALVQRSLDDKVDIFGFGNVLGPKFKAENIPVATAFIRK
                     VQRETGAEINLVKDCWERPRPFVVSKDVHPPGTFAQDMMIKPGTVEKNTAPHDAGSPC
                     KPVEQPAVSYSYPSGNANFGTTVAILLAAMVPEKRGEIFARGWEYGDNRLVAGVHFPS
                     DIESGRISATAMVAVMMQNPAFKSDFTAAKAELRNALGLTP"
     gene            complement(3637..4155)
                     /locus_tag="VGM72_13420"
     CDS             complement(3637..4155)
                     /locus_tag="VGM72_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266313.1"
                     /translation="MKYPHIAGILMAGALAATTLTVEVAQGAPAPAAPAGGGLTILEF
                     KPAFDDLMTMLVQPRHIKLYAAGQQKNWQLAAFELNEMRQALRRIGQTIPNYRSFKVD
                     NSVASLFTPSAEAMDAAIKAKDPAKFNSAYGELTASCNACHQGMEHPFLVVKVPDSAA
                     AATYADQDFAPK"
     gene            complement(4252..4788)
                     /locus_tag="VGM72_13425"
     CDS             complement(4252..4788)
                     /locus_tag="VGM72_13425"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY4266314.1"
                     /translation="MSKARIVAAALLFAGAAHAATPAPPKLGRPATAGDIAKIDISIA
                     PDGKGLPAGSGSVAQGAAIFQQKCAVCHGAMAGGTPSGDRLVGGVGSLATPNPVKTVN
                     SNWPYATTVFDYIRRAMPITNPQSLQNDEVYALTAYILSFDNVVPKDAVLDAASLPKV
                     QMPNRNGFVNWEPRLIRP"
     gene            complement(4781..>5202)
                     /locus_tag="VGM72_13430"
     CDS             complement(4781..>5202)
                     /locus_tag="VGM72_13430"
                     /inference="COORDINATES: protein motif:HMM:NF015369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266315.1"
                     /translation="MPDGKARQFMLQMEPKSVILKPSFGIAMQGPGYYEISGLAWSGL
                     GRVKQVDISADGGKSWTAAALNGPVLSKALTRFRLPWMWSGEPVTLMSRTTDEKGIVQ
                     KTHAEWAAQYAPRQNFHFSAIQSWAVDAGGKVSNIYV"
BASE COUNT          975 a         1614 c         1637 g          976 t
ORIGIN      
        1 acaatttttt ggcgatgcag caggcgattg tcgattccgg cctggaagaa aaagacatcg
       61 tcaatgagcg cacggggctg atcatgggtt cgggcggccc ctcgacccgg gcgattgttg
      121 ccgccgccga caccgcgcgc gtcgagcccg tcaaagggcc caagcgcgtg ggacccttcg
      181 tggttcccaa agccatgtca tccacctgtt cggcggttct ttccacctgg ttcaagatca
      241 agggtctttc ctattccatc agttcggcct gcgcgaccag cgcccattgc atcggcaacg
      301 cggcggaact gatccaatgg ggcaagcagg acatcatatt cgccggcggg ggcgaggagc
      361 tggattggtc gctctccgta ttgttcgatg cgatgggcgc gatgtcctcg catttcaacg
      421 cggcgccgga gcgcgccagc cgcgcctatg acaagaacag agacggcttc gtgatcgcgg
      481 gcggaggagg cacgctggtg ctggaagagt tggagcacgc caaagcccgc ggcgcgaaaa
      541 tctatgccga actggtgggc tatggcgcaa cttcggacgg ccacgacatg gttgccccat
      601 ccggggaagg cgctgtgcgc tgcatgcgtc aggcgctcgc gaccgtgaaa gcgccgctcg
      661 actatgtgaa tgcccacggc acctcgactc cggtcggcga tgcggtcgaa atcgccgcca
      721 tgcgcgaggt cttcgaaaaa aacatgccgc cggtgaattc gaccaaatcc ctgaccggcc
      781 attcgctcgg cgcagccggg gtgcatgagg cgatctattc gctcctgcag atgcaaagcg
      841 gcttcatcgc ggaatccgcg aacatcgagg agatcgatcc ggaagtggcc gacgcaccca
      901 tcgtgagaaa gcgcatcgac aacgccaaac tccagaccgt gatgtccaac agtttcggtt
      961 tcggcgggac caacgcctcg ctggtgttcc agcgttacga tgcgtgaaat ttttgggcgt
     1021 gaaacttttg ggcgtgaaag atattcgcgg gcctggggga acaatggctg aagcctatgc
     1081 cgcgaaaccc ggcacattga tggcgggaaa acgcggtctc atcatgggag tcgcgaacga
     1141 ccattccatc gcctggggca tagcgcgcac gctggcggca cacggggccg agctggcttt
     1201 cacgtatcag ggcgacgcgc agggcaaacg cgtgcgtccg ctcgccgaaa gcatcggcgc
     1261 gcgcctcgtc attgcgtgcg acgtcggcaa cgatgccgag gtcgaaacgg gcttcgcgac
     1321 gctgcaagct gagtggggcg ggcttgattt tctggttcac gctatcgcct attccgacaa
     1381 gaacgagctc aagggccgtt atgtcgagac cagccggcag aatttcatca gcacgctgaa
     1441 catttcctgc tattccttca ccgatgtggc gcgtcgcgcc gctcttctga tgaagaatgg
     1501 cggcgcgctg gtcacgctca cctatggcgg cgcgagccgt gtgatgccga actacaacgt
     1561 catgggcgtg gccaaggcgg cgctcgaagc cagcgtgcgc tatctcgccg ccgacctcgg
     1621 caagcaggac atccgcgtca acgcgatttc ggcaggtccg atgcgcacgc ttgcgggctc
     1681 ggcggtcggc gacgcccgca tggtgttcaa atggaacaag gcacacagcc cgctcaagaa
     1741 gaacatcgag ctggatcacg tcggcggggc cgcgctgtac ttgctgtcgg acctttcggc
     1801 gggggtgacc ggcgagatcc atttcgtgga tgccggctac aacacagtcg gcatgccgcc
     1861 gaccgccgac ctcaaaggct ggatcgcgcc ggaaaacggc gaaccggaat agcgtttttc
     1921 aaaaacgccc gcattcgaga tgaaaacccc cgcacaacgc aaatcccgct tcgggccgcc
     1981 gatcaatgtc gaagaggaaa tgcgcaccgg cgccatcgcc tattggcagg gcgattatta
     2041 ccagagccgc gccgaccggg gcggcgtcag tctgctcgat tacatccacg cgctgttcgg
     2101 attgctgatg cagaagcgtc ccaaaacggt gttgatgatc ggctgtgcgg gaggatcgct
     2161 cgccaccatg ctggcacgga ttggcgtcgc ggtgacggcc atcgatgtga acccgcaggc
     2221 cttcgcgatc gcgcggcgct atttcgggct gccggacagc gtggagtgcc gggtcgccga
     2281 cggcttggat tatctggtct cgacgcggcg tatgttcgat gccgtcgcgc tcgatgcctt
     2341 ccacggcgga cgcgtggatg aggatttgat cagcgacgtg ttcttcgcgc gcacggcgaa
     2401 acgcctgcgc aaaaatggcg cggttttcgc gaacatccat gtgctcgacg attccgacgg
     2461 cgcagccgac cgtgccgccg cgcacatggc ggcggcgttt tcggacgtgc gcatcctgga
     2521 tcgccgccgc gcgaaaccgc gcaacgcgat tgtcatggcg ggcgcggtcc gcggcctgaa
     2581 gaagccggcg ctgctgatgc cgcccgcgat cgaaaccggc aaaatcaccc gcgtgctcga
     2641 tgccatgcag ttccgtccgc cgctgcgttc gcaaagatag gctttgcgga aaacggatgt
     2701 gtcttccatg ccgaggcggg cagttcactg cccgcctttt tcatttgcgc cgcgccatcc
     2761 cttctggggg attgcgcacg atggatcgtc taggatgcgg gggcacgtat tcgcgcgctc
     2821 gatcttcagg gagaacatcg gccatgcgca ctcttgttgc acaagcgatt cttctgttcg
     2881 cgctcagcct cgcaccggct tgcgcactct ccagcgaacc ctatctgaag ccggggcaag
     2941 tcgatgcgct tgttctgatt gcgccacccc cggcaggcga gatccaggcg cgggacctcg
     3001 ccgatgtgct gcgcgcgcaa gcggcccgca cgcctgcgct tgtgcaacgc tccctcgacg
     3061 acaaggtgga tattttcggt ttcggcaatg tgctgggccc gaagttcaag gccgagaata
     3121 tcccggtcgc cacggccttc atccgcaagg tgcagcgcga gaccggcgcc gagatcaatc
     3181 tggtcaagga ttgctgggaa cggccgcggc ccttcgtcgt cagcaaggac gtgcatccgc
     3241 ccggcacctt cgcgcaggac atgatgatca agccgggcac ggtcgagaag aacaccgcgc
     3301 cccatgacgc gggatcgccc tgcaagccgg tggagcaacc cgccgtcagc tattcctacc
     3361 cgtccggcaa tgccaatttc ggcaccacgg tcgccatcct gctggccgcc atggtgccgg
     3421 agaaacgcgg cgaaatcttt gcgcgcggct gggaatatgg cgacaatcgt ctggtggcgg
     3481 gcgtgcattt cccgtcggat atcgaatcgg gacggatttc ggccaccgcc atggtcgccg
     3541 tgatgatgca aaatccggcc ttcaaaagcg attttaccgc ggcgaaggcc gaattgcgca
     3601 acgcgctcgg gctgacgccc taatcgttgt atcggcttat ttcggcgcga aatcctggtc
     3661 ggcataggtc gcggcagcag ccgaatccgg caccttgacc accaggaagg gatgttccat
     3721 cccctgatga caggcgttgc aggaggcagt caattcgcca taggcactgt tgaatttcgc
     3781 ggggtccttc gctttgatcg ccgcatccat cgcctcggcg cttggggtga aaagcgacgc
     3841 taccgaattg tccaccttga agctgcggta gttcggaatc gtctgaccga tccggcgaag
     3901 ggcctgacgc atctcgttca actcgaaagc cgcgagctgc cagttcttct gctgaccggc
     3961 cgcgtataat ttgatgtgcc gaggctggac cagcatggtc atgagatcgt cgaaggccgg
     4021 cttgaactcg aggatcgtca accccccgcc cgctggagca gccggcgcag gcgcgccttg
     4081 cgcgacctcc accgtcaggg tggttgcggc caaagccccg gccatcaata ttccggcgat
     4141 atgaggatat ttcataggtg ccccgtttgg gtgatttgtc tcaccaaaga gacgcttcac
     4201 gcaccgcata cccccatagc cgcgtatctg acggccggag gaccctggct tctagggcct
     4261 gatcagccgt ggctcccaat tcacgaagcc gttgcgattg ggcatctgca ccttgggcaa
     4321 ggatgcggcg tccaacaccg catccttggg cacgacattg tcgaaggaga gaatataggc
     4381 cgtgagcgcg tagacttcgt cattctgcag ggattgcgga ttggtgatcg gcatggcgcg
     4441 gcggatgtaa tcgaacaccg tggtggcata gggccaattg gaattcaccg tcttcaccgg
     4501 attgggtgtc gcaagcgaac cgacgccgcc gaccaggcgg tcgccgctcg gcgtgcctcc
     4561 ggccatcgcg ccgtggcaga ccgcgcattt ctgctgaaag atcgccgcac cttgcgcgac
     4621 ggaacccgag cccgcgggca agcccttgcc gtcgggggcg atgctgatgt cgattttggc
     4681 gatgtcgccg gccgtcgccg gccgtcccaa tttcggcggc gccggcgtcg cggcatgggc
     4741 ggcacccgca aacaaaagcg cggcggcgac aatccgagct ttagacatag atgttgctca
     4801 ccttaccgcc ggcatcgacc gcccagcttt ggatggcgct gaaatggaaa ttctgccgcg
     4861 gcgcatattg ggccgcccat tcggcatgag tcttctgcac gattcccttt tcgtcggtgg
     4921 tgcggctcat caaagtgacg ggctcgccgc tccacatcca gggcagacgg aatcgcgtca
     4981 gcgccttgga gagcaccggc ccattcagtg cggcggcggt ccagctcttg ccgccgtcag
     5041 cggagatgtc gacctgtttc acccggccca atcccgacca cgccagaccg gaaatctcgt
     5101 aatagcccgg cccctgcatg gcgatgccga aggacggctt caggatcacc gattttggct
     5161 ccatctgcag catgaactgg cgcgctttgc cgtccggcat ca
//
LOCUS       DASYEG010000316         4208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4904997, whole genome shotgun sequence.
ACCESSION   DASYEG010000316 DASYEG010000000
VERSION     DASYEG010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4904997"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..3036)
                     /gene="mutS"
                     /locus_tag="VGM72_13435"
     CDS             complement(259..3036)
                     /gene="mutS"
                     /locus_tag="VGM72_13435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY4266316.1"
                     /translation="MAYASERDSDGERDNDGARSLNVPDPSVAEGATPMMAQYLEIKA
                     AHADYLLFYRMGDFYELFFEDAVKAAAALDIALTRRGKHMGDDIPMCGVPVHAAEAYL
                     EKLIRKGFRVAVCEQMEDPALAKKRGAKSPVKREVVRLVTPGTLTEDSLLDARAPSVL
                     AALGRAGGAFALAWADISTGDFAVEPVVAPDLAQALARLSPRELLAPDSLLSEEATAP
                     AFKELGPRLTLRPVSNFDSDNGERLLKTLYGVAVLDGLGAFSRAELSAAGALVAYLEL
                     TQKGKRAGLKALKREAESGRMGIDAATRRNLELTETLSGERRGSLLATIDGTVTAAGA
                     RALHQRLTSPLTEPAAIAARLDAVAFFLTKTGLREAVRASLKAAPDIARAAARIGLGR
                     GGPRDLGAIRDGLKAARVLRRIFGDLREVLQPAPGELARAHGELTEALAALNPLADRF
                     EQLLVPEPPFFARDGGFIQGNAHAALDEARSLRDESRRVIAALESRYRGETGIASLRI
                     KHNAVLGYFIEVTATHADKLLSAGAGTFRHRQTVASAVRFATDELAGLAMKIAQAGET
                     ALAIELELFDALAQSVRAASGDLSRIADALAVLDVAAALANLAEQRNYARPRIDDSLT
                     FRIVRGRHPVVEAALASDHRGPFTANGSDLSPGGTEGGPGRLWLVTGPNMAGKSTFLR
                     QNALIAILAQMGSYVPAESAHIGAVDRLFSRVGAADDLARGRSTFMVEMVETAAILHQ
                     AGERSLVILDEIGRGTATFDGLAIAWAVVEHLNAVNKSRSVFATHYHELVALCERLKD
                     VACATMRVKEWKDNVVFLHEVAPGAADRSYGIQVAKLAGLPGAVTARATEVLRALEEG
                     REGHKPLARIDDLPLFAEKLREPKQATPKSSALEDALRAADPDSLTPKAALELLYALK
                     RLAADPSTGSR"
     gene            3187..>4208
                     /locus_tag="VGM72_13440"
     CDS             3187..>4208
                     /locus_tag="VGM72_13440"
                     /inference="COORDINATES: protein motif:HMM:NF015882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEY4266317.1"
                     /translation="MAERPSFTAQEALDFHAGDKPGKLEIRATKPMATQRDLSLAYSP
                     GVAVPVLAIARDPDLAYVYTTRANRVAVISNGTAILGLGDLGALASKPVMEGKAVLFK
                     RFADIDAIDLEIGTADVESFIGAVRYLGPTCGGINLEDIKAPDCFIIEDRLRELMDIP
                     VFHDDQHGTAIITAAGLINALQLTGRAIENTKMVVNGAGSAGIACLNLMVAMGLSRSN
                     VILCDTKGVIYSGRKEGMNQWKSPYAAETNARTLGDALDGADVFIGLSAKGAMTQEMV
                     EKMAKEPLIFAMANPDPEITPEEVRQVRGDAIIATGRSDYPNQINNVLGFPYIFRGAL
                     DVAATT"
BASE COUNT          741 a         1399 c         1355 g          713 t
ORIGIN      
        1 aaatcggccg gcgccacgat gtagagcagc gtgacgatgc cgccggtgtg ctgtgcgcgg
       61 cgcgcggcaa aacgcagcgc gactttgcat tcggtcgtct gatcgacgac gacgaggaat
      121 ttgcgccggt tgggcggaag cgtttgcagg ccggaaacat cgctcatcgg gctctatcgt
      181 gcctgcttgc cgtccggctc gcaaggtgtc gcgcgctcat gcttcgacaa gctcagcatg
      241 gggaaaagat acggtccttc accttgagcc tgtcgaaggg tcggcggcga ggcgcttcag
      301 cgcgtagagc aattccaaag cggccttcgg cgtgaggctg tcgggatcgg cggcacgcag
      361 cgcgtcttcc agggcgctcg atttcggcgt ggcttgtttc ggctcgcgca atttttccgc
      421 gaacagcggc agatcgtcga tgcgcgcgag cggcttgtga ccttcgcgtc cctcttccag
      481 cgcgcgcagc acttcggtcg cgcgtgccgt caccgcgccc ggcaatcccg ccagtttcgc
      541 gacctggatg ccataggagc ggtccgccgc gcccggcgcg acttcatgca ggaagacgac
      601 attgtccttc cattccttca cccgcatcgt ggcgcaggcg acatctttca gcctttcgca
      661 gagcgccacc aactcgtgat aatgcgtcgc gaaaacgctg cgcgatttgt tgaccgcgtt
      721 cagatgctcc accaccgccc aggcgatggc gagcccgtcg aaggtcgccg tgccgcgacc
      781 gatctcatcc aggatcacca gcgaacgctc gcccgcctga tggaggatgg ctgcggtctc
      841 caccatctcg accatgaagg tggagcgccc gcgcgccaga tcgtcggccg cgcccacccg
      901 gctgaacagg cgatccaccg cgccgatgtg agcggactcc gcgggcacat aggagcccat
      961 ctgcgcgaga atcgcgatca gcgcgttctg ccgcaggaag gtggatttgc cggccatatt
     1021 gggccctgtc accagccaca gccggcccgg gccgccttcg gtgccgcccg gcgacaaatc
     1081 gctgccattg gcggtgaagg ggccgcgatg atcgctggcg agggccgctt ccaccacggg
     1141 atggcggccg cgcacaatcc ggaacgtcag ggaatcgtcg attctgggcc gcgcgtaatt
     1201 gcgttgctcg gccaggtttg cgagtgcggc cgccacgtcg agcacggcca gcgcatcggc
     1261 gatgcgcgaa agatcccccg acgcggcgcg cacggattgc gccagcgcat cgaacaattc
     1321 cagttcgatg gcaagcgcgg tttcaccggc ttgcgcgatt ttcatcgcga ggccggcgag
     1381 ttcatcggtg gcgaagcgca ccgccgaggc gaccgtctgg cgatggcgga atgtccctgc
     1441 gcctgcgctg agcaatttgt ccgcatgggt ggcggtgact tcgatgaaat agccgagcac
     1501 cgcattgtgc ttgatgcgca acgacgcgat gcccgtctcg ccgcgatagc gcgattccag
     1561 cgcggcgatc acgcggcgcg attcatcgcg caaggagcgc gcctcgtcga gcgcggcatg
     1621 ggcgttgccc tgaatgaagc cgccatcgcg cgcgaagaag ggcggctccg gcacgagcaa
     1681 ttgttcgaac ctgtccgcca gcggattgag ggctgccagc gcctcggtca gttcgccatg
     1741 tgcgcgcgct aattcgccgg gcgcgggctg cagaacctcg cgcaaatctc cgaaaatgcg
     1801 ccgcaggacg cgcgcagctt tgaggccatc gcgaatagcg ccaagatcgc gtggcccccc
     1861 gcgccccaaa ccgatgcgcg cggcggcgcg cgcgatgtcg ggcgcggctt tcaggcttgc
     1921 gcgcaccgcc tcgcgcaagc ccgtcttggt caggaagaac gcaaccgcgt ccagacgcgc
     1981 agcgatggct gcgggctcgg tgaggggtga cgtcaagcgc tgatgcagcg cacgcgctcc
     2041 tgccgccgtc accgtgccat cgatggtcgc aagcaggctg ccgcgccgtt cacccgacag
     2101 agtctcggtc agttcgagat tgcgccgggt cgccgcgtcg atgcccatcc gccccgattc
     2161 ggcctcgcgc ttcagggcct tcagcccagc gcgcttgccc ttctgcgtga gctcgagata
     2221 ggcgaccagt gcgcccgcgg cggacaattc ggcgcgcgag aacgccccca acccatcgag
     2281 cacggcgacg ccataaaggg tcttcaacag ccgctcgcca ttgtcggaat cgaaattcga
     2341 aaccgggcgc agcgtcaggc gtggacccaa ttccttgaac gcgggcgctg tggcctcttc
     2401 ggacagcagg gaatcgggcg ccagcaattc ccgcggcgaa aggcgggcga gcgcttgcgc
     2461 cagatcgggc gcaaccaccg gttccaccgc gaaatcgccg gtcgagatat cggcccaggc
     2521 gagcgcgaag gcgccgcccg cccggcccaa agcggcgagc acggaagggg cgcgcgcatc
     2581 gagcagcgaa tcctcggtca gcgtgccggg cgtcaccagc cggacgacct cgcgtttgac
     2641 cggcgatttg gcgccgcgtt tcttggcgag ggcggggtct tccatctgct cgcagaccgc
     2701 aacccggaaa cctttgcgga tgagcttttc cagataggcc tcggccgcgt ggacgggcac
     2761 cccgcacatg gggatgtcgt cgcccatatg cttgccgcgt ctcgtcagcg cgatgtcgag
     2821 ggccgccgcc gccttgaccg catcctcgaa gaacagctcg tagaaatccc ccatgcggta
     2881 gaacaggaga taatccgcgt gcgccgcctt gatttcgaga tattgcgcca tcatgggcgt
     2941 ggcgccctcg gccacagacg ggtccggcac gttcaggctg cgcgcgccgt cattgtcgcg
     3001 ttcgccgtca ctgtcacgtt cggaagcgta ggccatggtt gaagctagca gatcgaaacg
     3061 atgcgcggac cgtccgtggg ccgcgcttgc gtcacttgtc cccaatttcg ccatgcccgg
     3121 gccagcccat tgaacgcgaa gccttgtcgg ctcaaactta agattaacaa ggagaatcgc
     3181 accgacatgg ccgaacgccc ttctttcacc gcacaggaag ccctcgattt ccacgccggc
     3241 gacaagcccg gcaagctcga aatccgcgcc accaagccga tggcgaccca gcgcgatctg
     3301 tcgctggcct attcgcccgg cgtcgcggtg ccggtgctcg ccatcgcgcg cgatcctgac
     3361 cttgcttatg tctatacgac tcgggcgaac cgggtggcgg tgatctccaa cggcacggcg
     3421 atcctggggc tcggcgatct cggcgcgctc gcctccaagc cggtgatgga aggcaaggcc
     3481 gttcttttca aacgctttgc cgatatcgat gcgatcgatc tggaaatcgg cacggccgac
     3541 gtcgaatcct tcattggcgc ggtgcgttat ctgggcccga cctgcggagg catcaacctc
     3601 gaagacatca aggcgccgga ctgcttcatc atcgaggacc gcttgcgcga gctgatggac
     3661 atccccgtct tccatgacga tcagcacggc accgccatca tcacggcggc cgggctgatc
     3721 aacgcgctgc aactcaccgg acgcgccatc gagaacacca agatggtggt gaatggcgcg
     3781 gggtccgcgg gcatcgcctg tctcaacctc atggtggcga tggggctgtc gcgcagcaac
     3841 gtcatcctct gcgacaccaa gggcgtcatt tatagcgggc gcaaggaagg gatgaaccag
     3901 tggaagtcgc cctatgcggc ggaaaccaac gcccgcacgc tgggcgacgc gctcgacggc
     3961 gcggacgtgt ttatcggcct ttccgccaag ggcgcgatga cgcaggagat ggtcgagaaa
     4021 atggcgaagg agccgctgat cttcgccatg gccaatcccg atcccgaaat cacgcccgaa
     4081 gaggtgcgtc aggtccgcgg cgacgccatc atcgccaccg ggcgttctga ctacccgaat
     4141 cagatcaaca atgtgctggg ctttccctat atcttccgcg gtgcgctcga tgtcgcggcc
     4201 accaccat
//
LOCUS       DASYEG010000317         2392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4905977, whole genome shotgun sequence.
ACCESSION   DASYEG010000317 DASYEG010000000
VERSION     DASYEG010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4905977"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..355
                     /locus_tag="VGM72_13445"
     CDS             164..355
                     /locus_tag="VGM72_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266318.1"
                     /translation="MTLSGCASMEAAPARSASLQNAIYVGGDTATVKPMNAASDGQKP
                     AVPPGEPKAMRFYWFLGGR"
     gene            complement(383..1543)
                     /gene="zapE"
                     /locus_tag="VGM72_13450"
     CDS             complement(383..1543)
                     /gene="zapE"
                     /locus_tag="VGM72_13450"
                     /inference="COORDINATES: protein motif:HMM:NF015902.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEY4266319.1"
                     /translation="MPQTPIERYSALLIAGKLKPDTAQARAAAALESLYRALKTYRPG
                     KSGWFGFSFGAARPAPKGLYIYGDVGRGKSVLMDLFCASVASQKKRRVHFNAFMAETH
                     ARIHEWRTLSPADRARRAEFVREAGDDPIAPVAKRIFSESVLLCLDEFQVTDIADAMI
                     LGRLFEKLLGDGAVIVLTSNTEPDRLYEGGLNRALFLPFVALIKDHLETIELNGPRDY
                     RLERMAGLNVYITPLGARADASMDAAWRRLTDTDHGAPLTLDVLQRALAVPEAASGVA
                     RFSFEALCGEALGAADYLALAQNFHTLLIDRIPQLGPERANEARRFTVLIDTLYDEKV
                     KLVCSAAVPPQELYAEGDNAPAFRRAVSRLMEMQSTDYLRLGPGARAMAATG"
     gene            complement(1568..>2392)
                     /locus_tag="VGM72_13455"
     CDS             complement(1568..>2392)
                     /locus_tag="VGM72_13455"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY4266320.1"
                     /translation="CLMSAGGAVSKEWSPKIAAAGAVVIDNSSQWRMDRQVPLVVPEV
                     NAAVLDEGIPKGIVANPNCSTAQLVVALKPLNDEAKIKRIVVATYQSVSGAGKDAMDE
                     LFRQTRAVFVADPIEKRKFTKQIAFNVIPHIDVFLDSGYTKEEWKMMVETQKILDPDI
                     QVSVTCVRVPVFIGHSEAVNIEFERPITAQRARALLREAPGVLVIDKHEDGGYITPIE
                     AAGEDATYVSRIRKDPTVENGLSMWIVSDNLRKGAALNAIQIAESLINRKLLKRAA"
BASE COUNT          449 a          734 c          758 g          451 t
ORIGIN      
        1 agttccggac aggtgcgcgg ggcatttgtt ttgactccgg gataagcata gctcagagtt
       61 gaccccaccc aaaaccaatg gggcgcggtt aaggggagaa cgcaacaccg cacagatggt
      121 gagcaccgtg accatatgca aatttgctct tctcggcgca gcaatgaccc tttcgggttg
      181 cgcctcgatg gaagctgctc cggcgcgatc cgcctccctg caaaacgcga tctatgtcgg
      241 gggcgatacc gccacggtca aaccgatgaa tgctgccagc gacgggcaga agccggccgt
      301 gccgccgggc gaacccaagg cgatgcgttt ctattggttc ctgggcggcc gctgaaaaca
      361 catcccattc tcgccggccg attcagccag tagccgccat cgcgcgcgca cccggaccga
      421 ggcgcagata atcggtgctc tgcatttcca tcagccgtga cacggcgcgc cgaaaagccg
      481 gcgcgttgtc gccttcggca taaagctcct gcggtggcac agccgcggag cagacgagct
      541 tgaccttctc gtcataaagc gtgtcgatca acacggtgaa acgccgcgct tcgttggcgc
      601 gttcggggcc gagctggggg atgcggtcga tcaggagcgt gtggaaattt tgcgccagcg
      661 ccagataatc ggccgcgccc aacgcctcgc cgcataacgc ctcgaaggaa aaacgcgcga
      721 cacccgatgc cgcctccggc acggcgagag cgcgctgcag gacgtcgagg gtaagcggcg
      781 cgccatggtc cgtatcggtc agccgccgcc atgcggcatc catgctcgca tcggcgcgcg
      841 ccccgagtgg ggtgatatag acgttcaacc ccgccatgcg ttcgagccgg taatcgcgcg
      901 gaccattgag ttcgatggtt tcgaggtggt ctttgatcaa cgcgacgaag ggcaggaaga
      961 gcgcacgatt gaggccccct tcataaagcc ggtcgggttc ggtgttcgag gtcagcacaa
     1021 tcaccgcgcc atcgccgagc agtttctcga acagccgtcc caggatcatc gcgtcggcga
     1081 tatccgtcac ctggaactcg tcgaggcaca ggagcacgct ttccgaaaaa atgcgcttgg
     1141 ccaccggcgc gatcggatca tcgcctgcct cgcgcacgaa ttccgcacgg cgcgcccgat
     1201 ccgccgggga aagagtgcgc cattcatgaa tgcgggcgtg ggtttcggcc atgaaggcgt
     1261 tgaagtggac gcggcgtttt ttctggctgg cgacgctcgc gcagaacaga tccatcagca
     1321 cggatttgcc acgcccgaca tcgccgtaga tgtaaagtcc cttgggcgcg gggcgcgcgg
     1381 cgccaaacga aaatccaaac catccgcttt tgccggggcg ataggtcttg agcgcgcggt
     1441 acagcgattc cagcgcggcg gcggcgcgcg cctgcgccgt gtcgggcttg agcttgccgg
     1501 cgatgagcag cgcggaatag cgctcgatcg gtgtttgcgg catgaaacgg cgttccgccg
     1561 cgctccctta cgccgcgcgc ttcaggagct tgcggttgat gagcgattcg gcgatctgga
     1621 tcgcgttcag cgccgcgccc ttgcgcagat tgtccgagac gatccacatc gaaaggccgt
     1681 tctccactgt cggatccttg cggatgcgtg agacataggt tgcgtcttcg ccggccgctt
     1741 cgatgggggt gatgtagccg ccgtcctcat gcttgtcgat gaccagaacg cccggcgcct
     1801 cgcgcagcaa tgcgcgcgcg cgctgcgcgg tgatcggacg ctcgaactcg atgttcaccg
     1861 cttcggaatg accgatgaag accggcacgc gcacgcaggt gacggagacc tggatatcgg
     1921 gatcgagaat tttctgcgtc tcgaccatca tcttccattc ttccttggtg tatccggaat
     1981 cgaggaagac atcgatgtgc ggaatgacgt tgaaggcgat ctgcttggtg aatttccgct
     2041 tttcgatcgg atcggcgaca aagaccgctc gcgtctggcg gaacaattcg tccatcgcgt
     2101 ccttgcccgc gcccgacacc gattgatagg ttgcaaccac gatgcgtttg attttggctt
     2161 cgtcgttcag cggcttcaac gccacgacaa gctgcgccgt cgaacaattg ggattggcga
     2221 cgatcccctt ggggattccc tcgtcgagga cggccgcgtt cacttcgggc acgaccaggg
     2281 gcacttggcg atccatgcgc cattgcgagg aattgtcgat cacgacggcg cccgcggcgg
     2341 cgattttggg agaccattcc ttggagacgg cacctccggc cgacatgagg ca
//
LOCUS       DASYEG010000318         4158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1434891, whole genome shotgun sequence.
ACCESSION   DASYEG010000318 DASYEG010000000
VERSION     DASYEG010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434891"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VGM72_13460"
     CDS             complement(<1..989)
                     /locus_tag="VGM72_13460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY4266321.1"
                     /translation="MQENNDGRESRSRQQAAARRRPTVLVLTTLGPNGMGGIDRLTDG
                     IRRHCATAVPDLELIFLTTRGPGLLLLSPLYSLRAALLLIWYRLTRGRSVCHVNLSSR
                     GSTIRKLALSWLARHLGHATVIHLHGSRFREYFQGVGALQRRAIIHMFQEARTVIVLG
                     QVWRDFVRASFNLDDSRLLVLPNATDARPIEQAREETPPLILFLGRIGIRKGVPVLLT
                     ALAALAKTKLAWRAEIAGDGDIAPYLAAAEASGLTGRVVFPGWLPESEAHARLSRAAV
                     LVLPSEAEGLPISIIEAFAWGIPVIATPVGSIPDILQNENQGFVVPVGDSAALA"
     gene            complement(1168..2670)
                     /locus_tag="VGM72_13465"
     CDS             complement(1168..2670)
                     /locus_tag="VGM72_13465"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HEY4266322.1"
                     /translation="MDMRSPIRQTGTLGLSSAPVEFRATGHLQPQNLGYLSLLLDVAL
                     FAGAGLAARPWVAAGPSALMRMIQFATIALALMVFFGMCRAIRAHDPLNLLHSRALPR
                     ISKMVLCFAIPVLVPLAISLPFIPEGDPEVRSFASWLACFEVFSIVFAIFAHSLFQIS
                     LPVLGRTLFARQRVAVVGSGESAERLIRWVELSAPGLFEVIGVFDDRESQRTQGSAVA
                     YKIRGSTSDLVDVYKSSAFDKVVIALPHSAESRLLDLLRRLRQLPVDIVLAPDLMGFH
                     VADEKTAEMAGLKLLSLAERPIRAPQRLLKGAIDICAAAFLLLTLSPLLLAIAIAIKL
                     DSPGHVLFRQRRHGLGDRLFDVFKFRTMHADLGDPSGRKQTKRSDPRVTRVGAFLRKT
                     SLDELPQLLNVLMGEMSLVGPRPHSPHMLIGDKRHYEIVAEYSFRHRVKPGITGLAQV
                     SGYRGAVDTPDHLQARIDLDLYYIDHWSLWMDFKILCRTAIVCLSGMNAF"
     gene            3002..3601
                     /locus_tag="VGM72_13470"
     CDS             3002..3601
                     /locus_tag="VGM72_13470"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY4266323.1"
                     /translation="MSLINAVTNLGDLTTILPFVSTVLAVLILSQEWRAARWWAFAIG
                     LAMGTALIAKLGFIPCGRMAAWLNINSPSGHTASATAAYGGMAMLYAQSSNPPRNRAV
                     AVLTAIAIALLIGATRIILGAHTPEESVLGAAIGLASPLFLAIPKRLFERPLLRHRGW
                     LLLLPCLVLLALLGRHAGTEGWITMAAIRLAHWFGACAA"
     gene            3794..>4158
                     /locus_tag="VGM72_13475"
     CDS             3794..>4158
                     /locus_tag="VGM72_13475"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEY4266324.1"
                     /translation="MRRSKSFLPLFSLLAMLVLILASAMAPARADDSLYRINGGDTLH
                     ISVYGERDLDRDVMVEPDGGIAFPLVGNLNARGLTLGDLQHKIAEQLRTGNMLPNVTD
                     PEVTVSLVKSTGNSISVIG"
BASE COUNT          878 a         1188 c         1304 g          788 t
ORIGIN      
        1 gccaatgccg cgctgtcgcc gactggaacg acgaatccct ggttctcatt ctgcaggata
       61 tccgggatcg accccaccgg cgtcgcgatc actgggatgc cccaggcgaa ggcttcgatg
      121 atcgagatcg gcagcccttc cgcttcggaa ggcaagacca gaaccgccgc gcgggatagc
      181 cgcgcatgag cttcagattc cggcagccag cctggaaata ccacgcggcc ggtaaggccg
      241 ctggcctcgg cggccgcgag atagggagcg atatcgccat cgccggcgat ttccgcgcgc
      301 caggcaagtt tggtcttcgc cagcgcggca agcgccgtca aaagcaccgg gacgcctttg
      361 cgaatgccga tccgtccgag aaagagaatg agcggtggcg tctcctcccg agcctgttcg
      421 atcgggcgtg cgtcggtggc attcggcaag accaataggc gggaatcgtc gagattgaac
      481 gacgctcgga cgaagtcgcg ccatacctgc ccaaggacga tgacggtcct cgcttcttgg
      541 aacatatgga tgatcgcacg ccgttgaagt gctccgacgc cttgaaaata ttcgcgaaac
      601 cgcgatccgt gcagatggat gacggtggca tggccgagat gccgggcgag ccagctcaag
      661 gcgagcttgc ggatcgtgct gcctcgggag gagaggttga cgtggcaaac cgaccggccc
      721 cttgtcagcc ggtaccagat caagagcagg gcggcgcgca gggaatagag gggcgacagc
      781 agcaaaagcc cggggccgcg ggtggtgagg aatatgagct cgaggtcggg aacggcggtc
      841 gcgcaatggc ggcggatgcc atcggtcaat cgatcgatgc cgcccatccc gtttggcccc
      901 agggtcgtca ggacgagcac ggtcggccgt cgccgcgcag ccgcctgctg gcgcgaacgc
      961 gattcccgac cgtcattgtt ctcttgcatc tgccccacgc acgcggcctg cccgccgctc
     1021 tgccgcgacc ttttcgaacc acgataagga agtcgcaaca gcatcaaatc ttaaatgaaa
     1081 gcgggcgacg gcgtcgagca tctcgtattg gcgcccggga tcggatgttc gagagtttcc
     1141 aagggagcga ccgttccgat gcggccttta aaatgcattc atgccggaga ggcacacgat
     1201 tgcggttcgg cagaggatct tgaagtccat ccagagcgac caatggtcga tgtaatagag
     1261 atcgaggtcg atgcgcgcct gcaggtgatc gggcgtatcc acggcgccgc gatagccgct
     1321 gacctgcgca agcccggtga tgcccggctt gacccggtga cggaagctgt attccgcaac
     1381 gatttcatag tggcgcttgt cgccgatcag catatgcggc gaatgcggcc gcggccccac
     1441 cagcgacatt tcgcccatga gcacgttcag caattgcggc aattcatcga ggctggtctt
     1501 tcgcaggaag gcgccgacgc gcgtcaccct gggatcgctg cgcttggtct gtttccgacc
     1561 ggagggatcg ccgagatcgg catgcatggt gcggaacttg aacacatcga acaggcgatc
     1621 gccaaggccg tggcggcgtt gccggaaaag cacgtgtccg ggactgtcga gcttgatggc
     1681 gatagcgatc gccagcagga gcggtgaaag cgtcagcagc aggaatgccg ccgcgcatat
     1741 gtcgatcgcg cctttgagca acctttgcgg cgcgcggatt ggccgctccg ccaaagacag
     1801 caacttcagg cccgccatct ccgcggtttt ttcatcggcg acatggaagc ccatgagatc
     1861 tggcgccaat acgatatcga cgggcaattg gcgcaaccgt cgcagcaggt ccagcagccg
     1921 tgattccgcc gaatgcggca gggcgatgac aaccttgtcg aaggcggatg acttgtagac
     1981 gtccaccagg tccgaggtcg atccacggat cttgtaggcg acggcggagc cctgggtgcg
     2041 ctgactctcg cggtcgtcga acacgccgat gacttcgaac aggccgggag ccgagagttc
     2101 cacccagcga atcaaccgtt ccgcggattc tccggaaccg acgacggcaa cgcgttggcg
     2161 ggcgaacagg gtgcggccga ggactggcag ggagatctga aagaggctat gggcgaatat
     2221 ggcaaaaacg atgctgaaaa cctcgaagca ggccagccag gaggcgaaac tgcgaacctc
     2281 cggatcgccc tccggaatga acggcaggct gatcgcaagc ggaaccagaa cgggaatcgc
     2341 gaagcatagg accatcttgg agatgcgcgg cagcgcgcgg gaatgcagca ggttcaacgg
     2401 atcgtgggcg cggatcgcgc ggcacatccc gaagaacacc atcaaagcca aggcaatggt
     2461 ggcgaactgg atcatccgca tgagcgcgga cggccctgcc gcgacccacg gtctggccgc
     2521 cagtcccgcg cctgcgaaca gagcgacatc gagcaaaagg ctcaggtagc ccaggttttg
     2581 cggctgcaaa tgcccggtcg cccggaactc tacgggcgca gacgacaggc cgagcgttcc
     2641 ggtttgccgg atcgggctac gcatatccat gttcattacc ccagcagaga cgtcttgcct
     2701 ttgcgtgcac gcgtgcaatc gcgagacggt tattcaaaac gcccgcataa attttaggac
     2761 aaactgaaaa aaatggctgc agaatcctat aaattcacca ccacaatatt gtgattaact
     2821 atgttttcca gccctttggc aaagggtttt gcggacaaat ttttcaacga gccgttaaac
     2881 cggacataga catgaaggca ggattttgct gcaatgcagc aatcagcgct cggcgcaaac
     2941 agcgcgccat ctcttggcgt gcttaacagt tcagcggctc gcgcagcccg tcaggagagt
     3001 agtgtctttg attaacgcag taaccaatct cggggacctg acgaccatat tgccgtttgt
     3061 cagcacggta ttggcggtgc tgatcctgtc acaggaatgg cgggcggccc gctggtgggc
     3121 cttcgcgatc ggactggcga tgggcaccgc gctgatcgcc aagctcggtt tcattccgtg
     3181 cggccgaatg gcggcttggt tgaacatcaa cagccccagc ggccataccg cttccgcgac
     3241 ggcggcttat ggcggcatgg cgatgctcta tgcgcaaagc agcaatccgc ccaggaatcg
     3301 cgcggtcgcc gttctgacgg caatcgcgat tgcgcttctg atcggtgcca cgcgcatcat
     3361 ccttggtgcg catacgcccg aggagtcggt attgggcgcg gcaatcgggc tggcttcgcc
     3421 gctctttctg gcgatcccga aacggctctt cgaaaggccg ctattgcgcc atcgcgggtg
     3481 gttgctgttg ctgccctgct tggtgctgct cgcactattg ggtcggcatg caggcaccga
     3541 aggctggatc accatggcgg cgattcggct ggcacattgg tttggtgcct gtgcagctta
     3601 agaaaccttg atgtctttgg tcccgtcaat ggcgagtgca tatccgaatg taagcgactc
     3661 ggtgaagcat cgctttcacc agaaataaat gattaagacc ttgggaacaa aataaaaacc
     3721 acaggtattt agctgtatca taaaggaaat gtagattatt ttctgatgta atcgaccaga
     3781 tgaggtttca acgatgagaa gatcgaagag ctttctgcct ttgttttcct tactggccat
     3841 gctggtgctg attttggcgt cggcgatggc gcctgctcgc gctgacgaca gtctttatcg
     3901 gataaacggt ggcgatactc tgcacatctc ggtctacggc gagagggatc tcgatcggga
     3961 tgtcatggtc gaacccgatg gcggcatcgc gttccccctg gtgggcaacc tgaatgcgcg
     4021 agggctgacc ctgggtgatt tgcagcataa gatcgccgag cagctccgga ccgggaacat
     4081 gcttccgaac gtcaccgatc ctgaggtcac ggtttcgctg gtcaagagca cgggcaattc
     4141 tatttcggtg atcggtca
//
LOCUS       DASYEG010000319        11416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1434912, whole genome shotgun sequence.
ACCESSION   DASYEG010000319 DASYEG010000000
VERSION     DASYEG010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 11416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11416
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434912"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..688
                     /locus_tag="VGM72_13480"
     CDS             170..688
                     /locus_tag="VGM72_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266325.1"
                     /translation="MIGIERKGGVEARHCFFAAHQIQQRDAAAVMGLREIWAQCHGFL
                     VASQSFLEARKASQCGAAIEMGFRRCRIDREGPVQFALGRLEIAAPIKHHPQKMARIE
                     MIRRARKDGTADVFGLRKPPPAVEFGSILHLRGGHCRPHCVRCHDVTHLIFLILSLPK
                     GASNPHPESVDG"
     gene            707..928
                     /locus_tag="VGM72_13485"
     CDS             707..928
                     /locus_tag="VGM72_13485"
                     /inference="COORDINATES: protein motif:HMM:NF024858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEY4266326.1"
                     /translation="MLKKRSFALSGHRTSVALEPEFWAALEEDASELGLALAALVGRV
                     DAERGERNLASALRLHALKRASQKTAPPA"
     gene            complement(949..1533)
                     /locus_tag="VGM72_13490"
     CDS             complement(949..1533)
                     /locus_tag="VGM72_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266327.1"
                     /translation="MRKRHFSIAGGLGLAVLGAMPSAAQPLKFTAMNREVEGFLTGLW
                     AKTSAPADRPPTEAEVTDGLSHCDAPMIDPKNQDETVRALFPSDDASHGDVSMFRSGG
                     AFVLVERLQRPDGTPYPNLKRMSVSAVQQQNRIVVRFVSRGAWTGGAWSDTPATSAIW
                     GDAMLGDLDIGGNTEHVFVLGKDNTQITYVKCGS"
     gene            1592..1813
                     /locus_tag="VGM72_13495"
     CDS             1592..1813
                     /locus_tag="VGM72_13495"
                     /inference="COORDINATES: protein motif:HMM:NF025143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4169 family protein"
                     /protein_id="HEY4266328.1"
                     /translation="MGEIEDAAAMSEIVNLKRARKTKARLEAEGTAAANRARHGTAKA
                     VRALTKARSEQSAALLDSHRLERGKDLKD"
     gene            1961..3616
                     /locus_tag="VGM72_13500"
     CDS             1961..3616
                     /locus_tag="VGM72_13500"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY4266329.1"
                     /translation="MSGRIVPTLLSAVAAAALIHVAGASAQTPAAIAGTVSSTQEGAM
                     EGVIVTAKKEGATISISVVSDDKGHYAFPANRLEPGHYNIKIRAIGYIPNGRPATDVA
                     AGKTAALDLKLNKTDNIAMQMTSAEWLTSMPGTDEQKAFLQDCTTCHTLIRPLSSGFT
                     AADFMQIIPRMGTYAPGSQPQRPQKLLPGPRGNRGIVDAAKVKAAAEYLASVNLSKTG
                     SYTYPLQAFPRPKGRATHVIYTSYDLARPEAMPHDVVFHQGNVWYTDFGSQFVGKLDP
                     KTGKVVDYPIPVLKADEPKGVLELKPDQDGNLWASMMYQGGIARIDTKTGAVKTYKVP
                     DKWQGPNTQESMVSPANWHVDGFVWTNNQEDHSILRVNVKTGEWENLGVLKDQKGRTI
                     NGYDIPTDKDNNLYLLEFGGTKIGKIDAKTKTLQTWDPDLPHARPRRGHFDENGTLWY
                     AEYGSNAVGRFDPKKETITEWQMQVKWQMPYDALANKKGDVYTGSVMSDRIVRLDPKT
                     GEQIVYLLPSSTNIRRVDFDDAGGALWVGSNHGNQIVKMEPLD"
     gene            complement(3752..4405)
                     /locus_tag="VGM72_13505"
     CDS             complement(3752..4405)
                     /locus_tag="VGM72_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004894963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEY4266330.1"
                     /translation="MSDNFQPLNRQEMAVRLARDIPEGWYVNLGLGLPTLIPDYIPAG
                     REVVFHSENGVLGMGPVPPKDAVDTWLVNATKQNITLAPGASLFHHADSFGMIRGGHL
                     DLCVLGAYEVAENGDVANWATNENDAIPAVGGAMDLSAGSKRLWVLMEHVTKDGAPRI
                     VKRCSYPLTAAGVVKRVYTNLAVLDVTAAGFVVLDMAPGLSFESLQARTEAKLHRAP"
     gene            complement(4416..5096)
                     /locus_tag="VGM72_13510"
     CDS             complement(4416..5096)
                     /locus_tag="VGM72_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005222233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HEY4266331.1"
                     /translation="MIDKRVASITEALAGVKDGAVVLLGGFGVVGQPQELLLGLIETG
                     ARDLTIVSNNAGFGSPALPKLFATGRVAKLICSFPRPTPPPFEALYRAGKIKLELVPQ
                     GTIAERIRAAAVGVPAFFTPTGVGTRLGEGKETREFDGRTYVMERALHADVALIQAWR
                     ADRWGNLSYRGTGRNFNPVMAMAASLTIVQTRHMVELGELDPETIVTPGIFVNRVVTV
                     DSPDPPPA"
     gene            5246..6562
                     /locus_tag="VGM72_13515"
     CDS             5246..6562
                     /locus_tag="VGM72_13515"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY4266332.1"
                     /translation="MAAKRNAGNNRVVVIGAGFAGIEVARALGAAGISATLIDRRNYH
                     LFQPLLYQVAAAALSPADIAEPIRKMLRRYRSVKVVLGEVIAIDAAARSVTLASGKII
                     AYDWLVAATGATHSYFGHEEWEQDAPGLKTVEDARIVRSRLLLAFEQAEMERHAQEQR
                     RLMTIAVIGGGPTGVELAGAIAELAHFTLAKDFHRIRAQDAQVILFEAGERILAAFPE
                     NLAAYATRRLEKLGVTVRLRSPVQNITARGIVVAGTEIGVGAAIWAAGVTASNTGRLL
                     GVETTRNGQIAVNPDLSVPQREGVYVLGDLALLAGENGKPLPGLAQVAKQEGQYLGRA
                     LVKRILKGADSPPFRFHNRGNTAIVGRHAAVFDFGWWQLRGWAAWVFWAIIHVYLLVG
                     FEHRLLVAVQWLWRYLTYDSGSRLIVEKPEGFRSPAEPVRDKQNKV"
     gene            6600..8975
                     /locus_tag="VGM72_13520"
     CDS             6600..8975
                     /locus_tag="VGM72_13520"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY4266333.1"
                     /translation="MTTPSNPYADPLDEAFDSSSSEGDSAPAVEPAYLNGLNAEQREA
                     VLALDGPVLVLAGAGTGKTRVLTTRLGHILATRRAWPSQILSVTFTNKAAREMKERIG
                     ALIGGTVEGMQWLGTFHSIGARILRRHAELAGLKSNFTILDQDDQLRLIKQVIEAENI
                     DEKRWPARTLAVMIDGWKNRGLSPTDVPDGDAQAFAFGKGRNLYALYQKRLKSLNAAD
                     FGDLLLEPIAILRGNHEILADYQGRFRYMLVDEYQDTNVAQYLWLRLLAGANKNICVV
                     GDDDQSIYAWRGAEVENILRFERDFPGAKVIRLERNYRSTATILGAASGLIAANRARL
                     GKTLWTETADDGDKVRIQGVWDAEEEARNVASEAENLHRKGQIFEQMAVLVRASFQMR
                     EFEERFLALGIPYRVIGGPRFYERAEIRDALAYLRVIAQGDDDLAFERIVNKPKRGLG
                     DATIQALHNHARSVNVPLFAAAAELAETDELAPKARATLGALTRSFERWRQQAAHLPQ
                     HELAEMVLEESGYTDMLKADKSPESAGRLENLKELVRSMEPYESLTAFLEHVSLVMEL
                     EQAEGPVDKLNVMTLHAAKGLEFETVFLPGWEEGLFPSQRTLDEKGTAGLEEERRLAY
                     VGLTRAKLRVLVSFAANRRVHGQWQSALPSRFIGELPAAHVDNVANEGFYGNYGNSFR
                     DNQSGFGNQSPFSGAYDSPGWRRAQASRNSASPFANAYEGARGRKPTIDAKAKVMATA
                     DPGQSAYGVGERVFHQKFGYGRVVAVEGNKLLVDFDKAGSKRVMDGFVGKA"
     gene            complement(9000..10007)
                     /locus_tag="VGM72_13525"
     CDS             complement(9000..10007)
                     /locus_tag="VGM72_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266334.1"
                     /translation="MTKFGTANITGGLALLCVSAALLPALGAGTAQTTAIPQLMLPAT
                     EGWSGFIVRGSDPTAYLPEDYLQGRQSTARAISLGAKPSNVTAYLHGRTFNEWIAPVS
                     GIGPIADGPEHPFYNNNVAQVVGRNPTYRVADLTSDAAKNLMPWALDALKKQNALALA
                     GKNGETRQARCWETGVPDIHEAPQSLYIIQTPAKVLLYQGGRVRHIYLDVPHTKNPKP
                     SWYGESVGHYEGDTLVVDTIGFNDKTFVDGYRTPHTNRLHVVERFRVINGGKGLDVSF
                     TVDDPGTFYRPWSARRPRYRTPDRENFTGGMEEDSCAGNNDDKFNQGLEPVPQAETPD
                     F"
     gene            10146..>11416
                     /locus_tag="VGM72_13530"
     CDS             10146..>11416
                     /locus_tag="VGM72_13530"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 and transglutaminase domain-containing
                     protein"
                     /protein_id="HEY4266335.1"
                     /translation="MQSSRKARLLERAAAIVILSASICNTAIAQNTPARVRLETTDVD
                     VAPDGTATEINHRELQVFTAKAAMQLAQLPISYIETMQDIDVTEAYTLKGDGRKLAVD
                     PSGILTQQAPQAANAPLFTDLKQKVVIFPNVEVGDTLVLTKKRHDKQSYFKGRFIKQS
                     VFSPLTPVAQYDLTIAIPKSLSSTLENHAIEFQKSASGDKDVYKAHYENLNPETEDVA
                     AIAQYDRLPRYFISTFKDYDEMGRSYAALFGPKVAVSAKIKAQADTITAGITDRREQA
                     RAIYEWVSQHVRYVGIELGQGAIVPRDAELVLANAYGDCKDITVLLSALLKAKNIDSE
                     PALINAGNGYTLSTAPTLAELNHVIAWLPEFKMYADATANTVPFGMLPLFEYGKTVVL
                     AGNDAGLRQIPVLAADAGTITYTNVAKLDDT"
BASE COUNT         2243 a         3530 c         3544 g         2099 t
ORIGIN      
        1 cgcatagtcc gcgagcgctt cctcgaaacg tccggccgcg cacagcgcga cgccgcgatt
       61 gttgagcgca tgcgcgtaag cgggattgat ggcgatggcg cggccgtaat gcgcaatcgc
      121 ttcgtccaga cgatcgagct gactggccac ggtcgttgat ggcttcggcg tgattggcat
      181 cgagcgcaag ggcggcgtcg aagctcgcca ttgtttcttc gctgcgcacc agatccagca
      241 gcgcgacgcc gcggcggtaa tgggcctgcg cgagatctgg gcgcaatgcc acggctttct
      301 cgttgcatcg cagagcttcc tcgaagcgcg caaggcttct cagtgcggtg ccgcgatcga
      361 gatgggtttc cgccgatgcc ggatcgatcg cgagggcccg gtccagttcg cgctcggccg
      421 cctcgaaatc gccgcgccaa taaagcacca cccccagaag atggcgcgca tcgaaatgat
      481 ccggcgcgcg cgcaaggatg gaacggctga cgtgttcggc ctccgcaagc cgcccccggc
      541 ggtagaattc ggaagcatcc ttcatctccg cggcggtcat tgccgccccc attgcgtccg
      601 atgccatgat gtcactcacc tgatattcct catattaagc ttgccgaagg gcgcctcaaa
      661 ccctcaccct gagtctgtcg acggatgagg aggattgtgt aatccgatgc tgaagaaacg
      721 atccttcgcc ctgtccgggc atcgcaccag cgttgcgctg gaacccgaat tctgggcggc
      781 gctggaagaa gatgcaagcg agctaggact tgcgcttgcc gctttggtgg ggcgggtgga
      841 tgcggagcgc ggagagcgca accttgccag cgccttgcgg cttcatgcgt tgaagcgcgc
      901 ctctcaaaaa accgcgccgc cggcataacg attcaccggc atgacggttc aggagccgca
      961 cttcacataa gtgatctggg tattgtcttt cccgagcacg aagacgtgtt cggtatttcc
     1021 gccgatatcg agatcgccca gcatggcgtc tccccaaatc gcggacgtcg ccggcgtatc
     1081 gctccacgct ccgcctgtcc aagcgccgcg cgaaacgaac cgcacgacga tcctgttctg
     1141 ctgctggacg gcgctgacgc tcatgcgttt gagattcggg taaggggttc cgtccggcct
     1201 ttgcaggcgc tcgacaagga caaaggcgcc gccggagcga aacatagaga cgtctccatg
     1261 agacgcgtcg tcgctcggga agagagcgcg gacagtttcg tcctggttct tcggatcgat
     1321 catcggcgcg tcgcaatgcg agaggccatc ggtcacttcg gcttcggtcg gcggccggtc
     1381 ggctggcgcc gaggtcttgg cccagagccc ggtgaggaac ccttccacct cgcgattcat
     1441 cgcggtgaat ttcaggggct gcgcggcaga cggcatcgcg ccaagcacgg caaggccaag
     1501 ccctccggca atgctgaaat gacgctttcg cacggcgact ccgttccctt gcgaacccag
     1561 gggatagcac aacccattga gggaccctat tgtgggcgaa attgaggacg ccgccgcgat
     1621 gagcgaaatc gtgaatttga agcgtgcgcg caaaaccaag gcccgcctgg aggcggaagg
     1681 gaccgccgcc gcgaaccggg cccgtcatgg aacggctaag gcggtacgcg cgctcacaaa
     1741 agcgcgatcc gagcaatccg cggcgctgct ggattcccac cggctggagc gcggcaagga
     1801 cttgaaagac tagccgttcg gtttggttag cgcagctcgc gaaacctgct tgcaggctgg
     1861 acgtctctcc ctcttgagct ttcgtatggc gacgggtttg tgtattctcc gcggttgaac
     1921 ccattgccac ccatttcgcg acccagccaa agagggactc atgtccggga gaatagtacc
     1981 aacgcttttg tccgccgttg cggcggctgc tcttatccat gtggcgggcg cttcggccca
     2041 aacgcctgca gcgattgcgg gcacggtgag ttccacccag gaaggcgcca tggaaggggt
     2101 gatcgtcacc gccaagaaag agggcgccac catttccatc agcgtggtgt ccgatgacaa
     2161 aggtcattac gcatttccgg ccaatcggct cgaacccggt cattacaaca tcaagatccg
     2221 cgccatcggc tacatcccga acggccggcc cgcgaccgat gtggcggcgg gcaagaccgc
     2281 ggccctcgat ctgaagctga acaagaccga caacatcgcg atgcagatga cgagtgcgga
     2341 gtggctcacc tccatgccgg gcaccgacga gcaaaaagcg ttcctgcagg attgcacgac
     2401 ctgccacacc ttgatccgcc cgctgtcgag cggtttcacg gcggcagact tcatgcagat
     2461 cattccgcgc atgggcacct atgcgcctgg cagccagccg caacgtccgc agaaactgct
     2521 tcccggtccg cggggaaatc gcggcatcgt ggacgccgcg aaagtgaagg cggccgccga
     2581 atatctggcg agcgtcaacc tctccaagac cggctcctac acttatccgc tgcaggcctt
     2641 cccgcgcccc aagggccgtg ccacgcacgt gatctatacg agctacgatc tggcccgccc
     2701 ggaggcgatg ccccatgacg tcgtcttcca tcaaggcaat gtctggtaca ccgatttcgg
     2761 cagccagttc gtcggcaagc tcgaccccaa gaccggcaaa gtcgtcgact atccgattcc
     2821 cgtgctgaag gccgatgaac cgaagggcgt cctcgaattg aagcccgatc aggacggcaa
     2881 tctctgggcg tccatgatgt atcagggcgg catcgcgcgc atcgacacca agacgggcgc
     2941 ggtgaaaacc tacaaggtgc cggacaaatg gcagggtccg aacacgcagg aatccatggt
     3001 ctccccggcc aattggcatg tggatgggtt cgtgtggacg aacaatcagg aagaccactc
     3061 gatcctgcgc gtcaatgtga agaccggcga gtgggaaaat ctcggcgtgc tgaaggatca
     3121 aaagggccgc accatcaacg gctatgacat cccgacggac aaggacaaca atctctatct
     3181 gctcgaattc ggcggcacca agatcggcaa gatcgatgcc aagaccaaga ctctgcaaac
     3241 ctgggatccc gatctgccgc atgcacgccc gcgccgcggc catttcgacg agaacggcac
     3301 gctgtggtac gcggaatacg gctccaacgc ggttggccgt ttcgacccga agaaggaaac
     3361 catcaccgaa tggcagatgc aggtgaagtg gcagatgccc tatgacgcgc tcgccaacaa
     3421 gaagggcgac gtttacaccg gctcggtcat gagcgaccgc atcgttcggc tcgatcccaa
     3481 gaccggcgag cagatcgttt atctgctgcc gtcgagcacc aatatccgcc gcgtcgattt
     3541 cgacgatgcg ggtggtgcgc tctgggttgg ctcgaaccac ggcaatcaga tcgtgaagat
     3601 ggagccgctg gattaagcgg cgtcctcagc gattgcgttg aacaagggcg ggcgccggta
     3661 acggccccgc cctttgtcca tgctcgaaac gcgcctggga tcagcgccgc aattcgggtg
     3721 ggacgatttg gaccggcagc cggcctgacg actaaggcgc gcggtgcagt ttggcctcgg
     3781 tgcgcgcctg caaggattcg aagctcagtc ccggtgccat gtcgaggacc acgaacccgg
     3841 cggccgtgac atcgagcacg gcgagattgg tgtagacgcg cttgaccacg cccgccgccg
     3901 tcaagggata ggagcagcgc ttcacgatgc gcggggcgcc gtccttggtg acatgctcca
     3961 tcagcaccca gaggcgcttg gagcccgccg acaaatccat cgcgccgccg accgcgggga
     4021 tggcgtcgtt ctcattggtc gcccaattgg cgacgtcgcc attctccgcc acctcatagg
     4081 cgccgagcac gcagagatcg aggtgcccgc cgcgaatcat cccgaaggaa tcggcgtgat
     4141 ggaacaggct cgcgcccggc gccagggtga tgttctgttt ggtcgcgttc actagccagg
     4201 tgtcgaccgc atccttgggc ggcaccggtc ccatgccgag cacaccattc tccgaatgga
     4261 atacgacctc gcggcccgcc gggatgtaat cggggatcag cgtcggcaga ccaagaccca
     4321 gattcacata ccagccttcc gggatgtcgc gcgcgagacg cacggccatc tcctggcggt
     4381 tcagcggttg gaaattgtcg ctcacaaatc cctcttcagg ccggtggcgg atcggggctg
     4441 tcgaccgtga ccacgcgatt gacgaaaatc cccggtgtca cgatggtttc gggatcgagc
     4501 tcgcccaact ccaccatgtg gcgcgtctgc acgattgtaa ggcttgctgc catcgccatc
     4561 acgggattga aattgcggcc tgtcccgcga tagctgagat tgccccagcg gtcggcacgc
     4621 cacgcctgaa tcagcgcgac atcggcatgc agcgcgcgct ccatcacata agtgcggccg
     4681 tcgaattcgc gtgtctcctt gccctcgcca aggcgcgtgc ccacgccggt cggcgtgaag
     4741 aaggccggca cgccgacggc cgccgcgcgg atgcgctcgg cgatggtgcc ctgcggtacg
     4801 agttcgagct tgatcttgcc ggcgcgatac agcgcctcga acgggggcgg cgtggggcgc
     4861 gggaaactgc agatcagttt ggcgacgcgc ccggttgcga acagtttggg cagcgccggc
     4921 gagccgaagc ctgcattgtt ggatacgatc gtcaggtcgc gcgcacctgt ctcgatcagg
     4981 ccaagcagca attcctgcgg ctgaccgacg acaccgaagc cgccgagcag cacgacggca
     5041 ccgtccttca ctccggcgag ggcttctgta atgctggcaa cgcgtttgtc gatcatgcgt
     5101 cgtgtgagca gttccatgtt ccgtgtgcgt tttggcgtgg cttgcacctt gtgacaagca
     5161 aaaaacttga tcttgcgacg ccgaggtgcc ggactgctta cgactagccc ttcctcgctt
     5221 acgactcggg cttcaaggac agccaatggc tgctaaacgc aacgccggga acaatcgcgt
     5281 ggtcgtcatc ggagccgggt ttgccggtat cgaagtggcg cgtgccctgg gggcggcggg
     5341 gatttccgcg acgctgatcg accggcgcaa ttatcatctg ttccagcctc ttctttatca
     5401 ggttgcggca gcggctctgt cgcccgcgga tattgcagag cccatccgca agatgctgcg
     5461 ccggtatcgc agcgtcaaag tcgtgctggg cgaagtgatc gccatcgacg cggccgcccg
     5521 gtccgtgacc ctggcaagcg gcaaaatcat tgcctacgac tggctcgtcg ccgcgaccgg
     5581 agcaacccac agctatttcg gccatgagga atgggaacag gatgcgcctg gtttgaagac
     5641 cgtggaggac gcgcgcatcg ttcgttcgcg gcttctgctc gcgttcgaac aggcggagat
     5701 ggagcgccac gcgcaagaac agcgccggct tatgacgatc gcggtgattg gcggcggccc
     5761 gacaggcgtg gaattggcgg gcgcgattgc cgaactcgcc catttcacct tggcgaagga
     5821 ctttcaccgc atccgcgcgc aggatgcgca agtcattctg ttcgaagccg gagagcgcat
     5881 cctcgccgcg tttcccgaaa atctggcggc ctatgcaacc cggcgcttgg aaaaattggg
     5941 cgtgaccgtg cgcctgcgtt cgcccgtgca aaacattacg gcgcgcggca tcgtcgttgc
     6001 cgggacggag atcggcgtgg gcgccgcgat ttgggcggcg ggcgtgaccg cgtccaacac
     6061 gggacgtttg ctgggggtcg aaaccacccg caacgggcag attgccgtca atcccgatct
     6121 gtcggtgccg cagcgcgaag gcgtttatgt gctgggcgat ctcgccttgc tggccggaga
     6181 aaacggcaag ccgcttcccg gtctggcgca ggtcgcaaag caggagggtc aatatctcgg
     6241 gcgcgcgctg gtcaagcgta tccttaaggg cgcggattcc ccgcccttca ggttccacaa
     6301 tcgcggcaat accgccatcg tcggacggca cgcggcggtt ttcgatttcg gctggtggca
     6361 gttgcggggc tgggcggcct gggtcttctg ggcgatcatc cacgtctatc tgcttgttgg
     6421 cttcgagcat cggcttctcg tcgccgttca atggctatgg cggtacctca cttacgacag
     6481 cggttcgcgg ctgattgtcg aaaagcccga aggattccgc agccccgctg aacctgttcg
     6541 cgacaagcag aacaaagtgt gacacagcgc cggtccaaac tgctagcatc cgcggaatca
     6601 tgaccacgcc ctcaaatccc tacgccgatc cgctggacga ggcgttcgat tcgtcttctt
     6661 cggaaggcga cagcgcgccc gcggtggaac ccgcctacct gaacgggctg aacgcggaac
     6721 agcgcgaagc cgtgctcgcg ctggacgggc cggtgctggt cttggccggc gcgggcaccg
     6781 gcaagactcg cgtgctgacg acacgtctcg gccatatcct cgccacgcgg cgcgcctggc
     6841 cgagccagat cctgtccgtg accttcacca acaaggccgc gcgcgagatg aaggagcgca
     6901 tcggcgcgct gatcggcggc acggtcgaag gcatgcaatg gctgggcacg ttccattcca
     6961 tcggcgctcg catcctgcgc cgtcacgcgg aattggccgg gctgaagtcc aatttcacca
     7021 tcctggatca ggacgatcag cttcgcctca tcaagcaggt gatcgaagcc gagaacatcg
     7081 acgagaagcg ctggcccgcg cgcacgctcg ccgtgatgat cgacggctgg aagaatcgcg
     7141 ggctcagtcc caccgacgtg ccggatggcg acgcgcaggc ctttgccttc ggcaaggggc
     7201 gcaatctgta cgcgctgtat cagaagcggc tgaaatcgct gaacgcggcg gatttcggcg
     7261 acctgctgct ggagccgatc gcgatcctgc gcgggaacca cgaaattctc gccgattacc
     7321 agggccgctt ccgctacatg ctggtggacg aatatcagga caccaatgtc gcgcaatatc
     7381 tctggctgag gctgctcgcg ggcgccaaca agaacatctg cgtggtcggc gatgacgatc
     7441 agtcgatcta tgcctggcgc ggcgcggagg tggaaaacat cctgcgcttc gaacgcgatt
     7501 ttcccggtgc gaaggtcatc cgtctcgaac gcaattaccg ctccaccgcc acgatcctgg
     7561 gcgcggcctc agggctcatc gccgccaatc gcgcccggct cggcaagacg ctgtggaccg
     7621 aaaccgcgga cgacggcgat aaggtacgca tacagggcgt gtgggacgcc gaggaggagg
     7681 cgcgcaacgt cgcatccgaa gcggaaaacc tgcaccgcaa gggccagatt ttcgaacaga
     7741 tggcggtcct ggtgcgcgcc tcattccaga tgcgcgaatt cgaagaacgc tttctggcgc
     7801 tcggcattcc ctatcgggtg atcggcggcc cccgtttcta cgaacgcgcg gaaatccgcg
     7861 atgcgctcgc ctatttgcgc gtgatcgcgc agggcgatga cgaccttgcc ttcgaacgca
     7921 tcgtcaacaa gcccaaacgc gggctcggcg atgccaccat ccaggcgctg cacaatcacg
     7981 cgcgcagcgt gaacgtgccg ctcttcgcgg ctgccgcgga gcttgcggag accgacgagc
     8041 tggcgcccaa agcgcgcgcg acattgggcg cgctgacgcg gtccttcgaa cgctggcgtc
     8101 agcaagcggc gcatcttccg cagcacgaat tggccgaaat ggtgctggag gaatcgggct
     8161 acaccgacat gctgaaggcg gacaaatcgc ccgaatccgc cggcagactc gagaacctca
     8221 aagagcttgt gcgctccatg gagccatatg aatcgctgac cgcgtttctg gagcatgtct
     8281 cgctggtgat ggagctggag caggccgagg ggcctgtcga caaactcaac gtgatgacgc
     8341 tgcatgcggc caaggggttg gaattcgaaa ccgtgttcct tcccggctgg gaggaaggcc
     8401 tgttcccttc gcagcgtaca ctggacgaaa aaggcaccgc tggattggag gaagaacgcc
     8461 gccttgccta tgtcgggctg acgcgcgcca aattgcgcgt cttggtcagt ttcgcggcga
     8521 accggcgcgt gcacggccag tggcagagcg cgctgccttc gcgcttcatc ggggaattgc
     8581 ccgctgccca tgtcgacaat gtcgcgaacg aaggcttcta cggcaattac ggcaatagct
     8641 ttcgcgacaa ccaatccggg ttcggcaatc aaagcccttt cagcggcgcc tatgacagtc
     8701 ccggctggcg gcgggcgcag gcgagccgta attcggcttc accttttgcc aacgcctatg
     8761 agggcgcgcg cggacgcaag cccaccatcg acgcgaaggc caaggtcatg gccaccgccg
     8821 atccgggcca atcggcctat ggtgtcggcg agcgcgtctt ccatcagaaa ttcggctatg
     8881 gccgcgtcgt ggccgtggaa ggcaacaagc tgctggtgga tttcgacaag gccggctcca
     8941 aacgcgtcat ggacggcttc gtcggcaagg cataggcccg ccttcgactt cgtgccggtt
     9001 cagaagtccg gggtctctgc ctggggaacc ggttcaaggc cctggttgaa cttgtcgtca
     9061 ttgttcccgg cgcaggaatc ttcttccatt ccgccagtga aattttccct gtccggtgtc
     9121 cggtagcgcg gccggcgcgc actccagggc ctatagaacg tgccgggatc atcgacggtg
     9181 aaggacacat cgagcccctt gccgccattg atgacccgga agcgctccac aacgtgcagc
     9241 cggttcgtgt gcggcgtgcg gtatccgtcc acgaacgtct tgtcgttgaa gccgattgta
     9301 tcgacgacca gcgtatcgcc ttcgtaatgc cccaccgatt cgccgtacca ggaaggcttg
     9361 ggattcttcg tgtgcggaac atccaggtag atgtggcgca cccgtccgcc ttgatacaga
     9421 aggacctttg ccggggtctg gatgatatac agggattgag gcgcttcgtg gatgtcgggg
     9481 accccggttt cccagcatcg tgcctggcgt gtttcgccgt tcttgcccgc aagggccaaa
     9541 gcgttctgtt ttttcaacgc atcaagggcc caaggcatca aattcttcgc ggcgtcgctg
     9601 gtcagatccg caactctgta ggtcgggttc ctgcccacga cttgcgccac attgttgttg
     9661 taaaaaggat gttccggacc gtctgcgata ggtcctattc cgctgaccgg cgctatccac
     9721 tcgttgaacg tacggccgtg aagataggcg gtcacgtttg acggtttcgc gccgagcgat
     9781 attgcgcgcg cagtgctttg gcggccttga aggtagtcct cgggaagata ggccgtggga
     9841 tcggagcctc gtacgatgaa cccgctccac ccctctgtcg ccggcagcat caactgggga
     9901 atggcggtgg tttgtgccgt gccggcgcca agggcgggga ggagcgccgc ggacacgcaa
     9961 agaagagcca agccgcctgt tatgttcgca gtcccgaatt tcgtcatgga tattccttcc
    10021 cgaacgccgc cctttagcgg gaatttatcg cattacagat gacatatccg taagagaaat
    10081 tcgcgctcag gttgcgataa tcttcccaaa cgtgcgaacg gctaaaccta ccgggggggc
    10141 gtttgatgca atcgtcgcgc aaagcccgtt tgctcgaaag agcggcggca atcgtcattt
    10201 tatccgcatc catttgcaat acggccatcg cgcagaatac gcctgcgcgt gtgcggctgg
    10261 aaacgaccga tgtcgatgtg gcgccggacg ggaccgctac cgaaatcaac catcgcgaat
    10321 tgcaggtgtt caccgccaag gcggcgatgc aactcgcgca attgccgatt tcgtacatcg
    10381 aaacgatgca ggatatcgat gtcaccgaag cctacacgct caaaggggat ggccggaagc
    10441 tggcggtgga cccgagcggg atcctcacgc aacaggcgcc ccaagcggcc aatgcgcccc
    10501 tgttcaccga cctgaagcaa aaggtggtca tttttccgaa tgtcgaagtc ggcgacacgc
    10561 ttgtgctgac caaaaagcgg cacgacaagc agagttattt caagggccgg ttcatcaaac
    10621 agtcggtgtt ttctcccctc acgccggtcg cgcaatatga tctgacgatc gccataccga
    10681 aaagcctgtc ctcgacgctg gaaaaccatg ccattgaatt ccagaaaagc gccagcggcg
    10741 acaaggatgt ctacaaggcg cattacgaga atctgaatcc agaaaccgaa gacgtggcgg
    10801 ccatcgccca gtacgaccgg ctgccgcgct atttcatctc gaccttcaag gactacgacg
    10861 aaatgggtcg atcctatgcg gccctgttcg gtcccaaggt ggctgtttcc gcgaaaatca
    10921 aggcgcaggc cgacaccatc acggccggca ttaccgaccg gcgcgaacag gcgcgcgcga
    10981 tctatgaatg ggtcagccaa cacgtccgct acgtcggaat cgaattgggc caaggggcca
    11041 ttgtgccgcg cgatgccgag cttgtgctcg ccaacgccta tggcgattgc aaagacatca
    11101 cggtcctgct ctccgccttg ctgaaagcaa agaatatcga cagcgaaccc gcgctgatca
    11161 atgccggcaa tggttacacg ctgtcgacgg cgcctacgct cgccgaactc aaccacgtga
    11221 tcgcgtggct tccagagttc aagatgtacg ccgacgcgac ggcgaacacc gtgccattcg
    11281 gcatgctgcc cctgttcgaa tacggcaaaa ccgtcgtgct cgccggcaat gatgcgggat
    11341 tgcggcaaat tccggttttg gccgctgatg ccggtaccat cacctatacc aacgtggcca
    11401 agctcgacga tacgat
//
LOCUS       DASYEG010000320         7380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4477142, whole genome shotgun sequence.
ACCESSION   DASYEG010000320 DASYEG010000000
VERSION     DASYEG010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 7380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7380
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4477142"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /gene="radC"
                     /locus_tag="VGM72_13535"
     CDS             complement(<1..473)
                     /gene="radC"
                     /locus_tag="VGM72_13535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEY4266336.1"
                     /translation="MLGGAGALPDYELLELLLFLSIPQRDTKPLAKALIARFGSFADV
                     IAAHPERLAEMEGVKDATVAALKTVEAAALRLSKAKIIGRPALASWEALLDYCAAAMA
                     RSTTEEFRVLFLDRKNVLVADEVHQRGTVDHTPVYPREVVKRALELGASAIILVH"
     gene            complement(541..1401)
                     /gene="map"
                     /locus_tag="VGM72_13540"
     CDS             complement(541..1401)
                     /gene="map"
                     /locus_tag="VGM72_13540"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY4266337.1"
                     /translation="MSDSFPELPPDLVLDRESIEVARRSAFAVATHRPEDFEGMRRAG
                     RLAAEVLDLLVPEVKPGVTTAALDKIAYDYVTAHGALPACLGYRGYRHTLCTSINHVV
                     CHGIPSDRPVKDGDILNIDVTVIIDGWHGDTSRMYYVGDVKRKSQRLVEITYESMMRG
                     IATVKPGATTGDIGYAIQSFAEDQERCAVVRDFCGHGLGRIFHALPNIVHYGRPGQGV
                     VLKPGMFFTVEPMINLGGYAVKVLSDGWTAVTRDRSLSAQFEHSIGVTQDGFEIFTLS
                     PKGWHAPPYV"
     gene            1563..2351
                     /locus_tag="VGM72_13545"
     CDS             1563..2351
                     /locus_tag="VGM72_13545"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEY4266338.1"
                     /translation="MTPPQGINAAVLVIGDEILSGRTQDTNVAAIARFLAPYGIDLAE
                     ARFVPDIEAEIVAAVNALRYRYTYVFTTGGIGPTHDDITADAIGAAFGLPVTHHPEAM
                     MLLSGRYTPGEFNERRQRMARTPAGASLIRNPISTAPGFQIENVFVMAGVPKIMQAML
                     EDIGPRLERSQPVVSRSLTVRLPEGRIAQALADLQSAHPAVSIGSYPFFAEVRPPLAG
                     AAGAAGTTLVIRGRDEAAVEAAAHGVAALARDHGVEPETGGRSH"
     gene            2515..2793
                     /locus_tag="VGM72_13550"
     CDS             2515..2793
                     /locus_tag="VGM72_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266339.1"
                     /translation="MIVKRVLAAALGGVLFAAAGVAAAGNFSSDRQVDFFAKGTHQFY
                     VWCSGASDYVATADGANAEEAQMRLYNAAKAAGKTSCWPVWQGRVAGS"
     gene            complement(2894..3787)
                     /locus_tag="VGM72_13555"
     CDS             complement(2894..3787)
                     /locus_tag="VGM72_13555"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4266340.1"
                     /translation="MIVVLILIFSLVALVAAGFVVLPLWRMRKADAGTGTHRPWLAIV
                     AGLSTMGLGLGAYAFLGQPQIALSDLTGPSRTDYKALITTLARRMPGRPGDVEGWALL
                     GRGYLALGNPSQAEKAFARAVEASKALHGVAPPDLLTSYGEAIAESAGQVTKEAEAAF
                     REALQEDPKDLASRYYVGLALATRGDKAGALQLWEGVLADAPPDTPWRSSLVDQLAAL
                     KAGSGGEAPNPMAMVAQLAARLETNPDDINGWVMLIRAYAVLGDKPKAVAALTRARSV
                     FSNQASAKAALAKVAADNSLN"
     gene            4194..4283
                     /locus_tag="VGM72_13560"
     tRNA            4194..4283
                     /locus_tag="VGM72_13560"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            complement(4378..5865)
                     /locus_tag="VGM72_13565"
     CDS             complement(4378..5865)
                     /locus_tag="VGM72_13565"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY4266341.1"
                     /translation="MAEQDPTQFGRRSFLNRVALAAGAGAIAPPLLGTGSATAAEAAA
                     PKGERGYTPKLAAYAAGLRYETVPAEVRQRIKDCITDTVAVILYGGKLPWSQMVIAHA
                     RHTGPGGKSHILGTGGVMVQAPSAALAHGTMTHAFELDNLTNPDSGSHPGASMLSSGL
                     AVAQERGSSGRDLITAMTAGAEVMIRVGRAAKGSLEPRGFHAPGTTGPFGGAVTVGQL
                     MGLDAGKMTNALGIAGSLSGGLLEFAHSNTGGMVKKLHLGRAAESGVLAASLASEGFT
                     GPDTVLEGEAGYLRAFCNEHDSTELTKGLGRDYASMDIMMKRFACHITAHRPVEAMLD
                     LMSEYKFSAGDVLAITVTGNRRMATTNNIPAPPDVLLAQYSIPFSVALSLYRNPIDPD
                     SFDDNVHRDPAILAMAARVKMLTAPGQEREDLTSLVTVTLKDGRSVSRTVTEFSGTPA
                     RPLDRAGLQEKFLLLTKRFPRADMQRMFDRLQNLENEKTLGWISV"
     gene            6010..6339
                     /locus_tag="VGM72_13570"
     CDS             6010..6339
                     /locus_tag="VGM72_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HEY4266342.1"
                     /translation="MNQLSAETRHGIEEALRCHGVCLATAMTHCLEMGGEHVKPQHFR
                     LMMDCAAMCQLAADLMAHKSQFHRQSCAFCADICEVCARDCEHMEGMEDCAAACRSCA
                     QSCRAMV"
     gene            6367..>7380
                     /locus_tag="VGM72_13575"
     CDS             6367..>7380
                     /locus_tag="VGM72_13575"
                     /inference="COORDINATES: protein motif:HMM:NF004834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4266343.1"
                     /translation="MDQAPTPPEPMRTGCCIAGGGPAGMVLGYLLARAGVEVIVLEKH
                     RNFLRDFRGDTVHPSTLEIMYELGLLEAFLKRPHQELRELSAIIGGVTVRIADFSHVP
                     AHCKFIALMPQWDFLNFLASEAKRFANFHLLMQAKVTDLLMEGTRIAGVAAKTPEGLL
                     QIRADLVVGADGRHSIVRDKAGLPRRDYGVPIDVLWMRLSKNPGDPATTLGRITAGAI
                     LVTLDRGDYWQCAFVIPKGETQNLRMRGLDAFRGLIARVAPELGNRVAELTDWNQIKL
                     LTVTVDRLKEWCRPGLLCIGDSAHAMSPIGGVGINLAVQDAVAAANILAVPLRSGTPP
                     LSVL"
BASE COUNT         1395 a         2254 c         2317 g         1414 t
ORIGIN      
        1 ttgtgcacca ggatgatcgc ggaggcgccc aattccaatg cgcgcttgac gacttcgcgc
       61 ggatagacag gcgtgtggtc cacggtgccg cgctgatgca cctcatcggc cacgagcacg
      121 ttcttgcggt ccaggaacag cacgcggaat tcttccgttg tactgcgcgc catggccgcc
      181 gcgcagtaat ccaacaacgc ttcccatgag gccagggccg gacgcccaat gatttttgcc
      241 ttcgacaggc gcagggccgc ggcttccacg gttttcaggg cagcgacggt cgcgtccttg
      301 acgccctcca tctcggccag acgttcggga tgtgccgcga tgacgtcggc gaaactgcca
      361 aagcgcgcga tcagcgcttt ggccaaaggc ttggtgtcgc gttgggggat cgacagaaaa
      421 agcagaagct caagaagctc gtaatcggga agggcgccgg ccccgcccag cacaaaacgt
      481 tcccgcagcc tctgacgatg gccgcggtaa tgcggaacct cgctattccc cgattcgtct
      541 tcagacgtag ggcggcgcgt gccagccttt gggcgagagg gtgaagattt caaagccatc
      601 ctgcgtcacg ccgatgctgt gctcgaattg tgccgagagc gagcggtcgc gggtaaccgc
      661 cgtccagccg tcgctcaaaa cctttaccgc ataaccgccg agattgatca tcggctcgac
      721 ggtgaagaac atgcccggct tcaacaccac gccctgcccc gggcggccat aatgcacgat
      781 gttggggagc gcgtggaaaa tccgtcccaa gccatggccg cagaaatcgc gcacgacggc
      841 gcagcgttcc tgatcttccg cgaaggattg gatggcatag ccgatatcgc cggtggtggc
      901 acccggcttc accgtggcga tgccccgcat catggattca taggtgatct cgacgaggcg
      961 ttgggatttt cgcttcacgt cgccgacata atacatgcgg ctggtgtctc cgtgccagcc
     1021 atcgatgatc acggtcacgt cgatgttcag gatatcgccg tccttgaccg gacgatcgct
     1081 cgggatgccg tggcagacga catgattgat cgaggtgcag agcgtatggc gatagccgcg
     1141 gtagccgaga caggcgggaa gcgcgccatg ggccgtcacg taatcatagg cgattttgtc
     1201 cagcgccgcg gtcgtgacgc cgggcttcac ttcggggacc agcaggtcga gcacctcggc
     1261 cgccagccgt ccggcccgcc gcatgccttc gaaatcttca ggccggtgcg tcgccaccgc
     1321 aaaagcggac ctgcgcgcca cctcgatgga ttcgcgatcc agcacgagat cgggaggaag
     1381 ttcgggaaag ctatctgaca tcggtcttgt ctcgccggtt gggcaatggg ctgtgggatg
     1441 ctcttcgctt aaattcgcca cgaatggcct gaattgtcac tatgggcctg aattgtcact
     1501 atcgctttta tacttgggct ttgagacgcg ccagcttcaa ggggccgccg gaggatatcg
     1561 tgatgacgcc accccagggg atcaacgcgg ctgtgctggt catcggtgac gagattctct
     1621 ccggacgcac gcaggacacg aatgttgccg cgattgcacg cttcctcgcg ccctatggga
     1681 tcgacctggc tgaagcgcgc tttgtgcccg acatcgaggc cgagatcgtg gccgccgtga
     1741 acgcgctgag ataccgctac acctatgttt tcactaccgg cgggatcggt cccacccatg
     1801 acgatatcac cgcagatgct atcggagcgg ccttcggcct gcccgtcacg caccaccccg
     1861 aggccatgat gctgctttcc ggccgctata cgccaggtga gttcaatgaa cgccgccaac
     1921 gcatggcccg cacgccagca ggtgccagcc tgattagaaa tccaatctcg acggctcccg
     1981 gtttccagat cgagaacgtg ttcgtgatgg caggcgtgcc caagatcatg caggccatgc
     2041 tggaagatat tggccccagg ctggagcgca gtcagccggt tgtgagccgc agcctgacgg
     2101 tccgccttcc cgaggggaga atcgcgcaag cattagccga tctccagtcg gcccacccgg
     2161 cggtcagcat cgggagctac ccgttcttcg cggaagttcg tccgccactg gctggggcgg
     2221 ccggcgccgc aggcaccacg ctagtgatac gcgggcggga cgaagcggcg gtggaggctg
     2281 ccgcgcatgg cgtggcggct cttgcacgtg atcatggcgt ggagcctgaa accggggggc
     2341 gttcgcatta atttgtcgta tgctattgga atttaaccaa acaaaatcaa agcctgcata
     2401 tcggacccac gacaaagccg ccgcatggga acaaattaag ggttgtcgcc cgggaccgcc
     2461 tatggcaagt tttttgccgt taggcatttc tcaccgaacc tgaggacaga acgcatgatc
     2521 gtcaaacgcg tcctagccgc ggctctgggt ggagttttat ttgcggccgc aggcgttgcc
     2581 gcggccggaa atttctcctc ggatcgtcaa gtcgatttct tcgccaaagg cacccatcaa
     2641 ttctatgttt ggtgctcggg tgcgagcgat tatgtggcga ctgccgacgg cgccaatgcg
     2701 gaagaggcgc agatgaggct ctacaacgcg gccaaagctg caggcaaaac ctcgtgctgg
     2761 ccggtctggc agggccgcgt ggcgggcagc tagacgcgcg cattcactct tgaatttaac
     2821 gaaaacagcg gcatagggcc gctgttttcg tttgggcccc cgttttcgtt tgaagatggg
     2881 tcagttggca gtgtcagttc agggaattgt ccgcagccac ctttgccaag gcagccttcg
     2941 ccgaggcctg attggagaag acactccgtg cgcgcgtcaa cgccgccacg gccttcggct
     3001 tatccccaag tacggcatag gcccggatca gcatgaccca gccattgata tcgtcagggt
     3061 tggtctccag ccgcgccgcc aattgggcga ccatcgccat cggattggga gcctcaccgc
     3121 cgcttcccgc tttcaaggcc gcaagctgat ccaccaatga gctccgccag ggcgtatcgg
     3181 gcggcgcatc ggcgagcact ccttcccaga gctggagcgc gccggctttg tcgccacggg
     3241 tcgcgagcgc caagcccaca taatagcgcg atgccagatc cttgggatct tcctgcaatg
     3301 cttcgcggaa agcggcttcg gcttccttgg tcacctgacc ggcggattcc gcaatggctt
     3361 ccccatagct cgtaagcagg tcgggtggcg ccacgccgtg gagcgccttg gacgcctcca
     3421 cagcgcgggc aaaagccttc tcggcctggg atgggttacc gagtgcgaga tagcctcgtc
     3481 ccaacagcgc ccagccttcg acgtcgccgg gccgccccgg catgcgcctg gcaagtgttg
     3541 tgatcagcgc cttgtagtcg gtgcgcgatg gtccggtcag atcggaaagc gcgatctgcg
     3601 gctggccgag gaacgcgtac gcgcccagcc caaggcccat ggtggatagc cccgcgacaa
     3661 tcgcgagcca gggacggtgg gtccccgtcc cggcatcggc cttgcgcata cgccacaggg
     3721 gcagcacgac aaagcccgca gcgacgagcg ccaccaaact gaagatgagg atcaggacga
     3781 cgatcatggg gccgaacgcc tagcacgggt gggctccgcg tcatagcagc tacggccctt
     3841 cacgcacgcc cgcttgacgc ccgcgtagga ttgagcttgg gcaggcagcc cgtggcggaa
     3901 tgatagacgc aatggactta aaagtccgac gccgctgcgc tacaagcact ggtcacgcgg
     3961 gcgtggcgaa atggtagacg caacggactt aagcgattga gtgctcgatg ggaaaccgtc
     4021 gatgcagaac tgctcaaaat cggggaaacc tttcaactgg cgatcccgag ccaagcccaa
     4081 aggatcaatc ccgcgggaag gtgtagagac tagacgggca gcacctaacc cccaaaccga
     4141 tccggggcat ggtgaaggga tagtccagac cacaaacacg ctttcgctga ttggcgatgg
     4201 cgtaggcggc gaaagccgta gctggtacga aaatccgtag gaccgaaagg tctgtgccgg
     4261 ttcaagtccg gccgcccgca ccatgacttt cgcttggtgc aatgctgcta acatcccttg
     4321 gctaccacga tgtttgctat tgaccgccgg ggtcgcccgc attggctcgc gaaagcgcta
     4381 gacgctgatc cagcccagcg tcttttcatt ctccagattc tgtagccggt cgaacatccg
     4441 ctgcatatcg gcgcggggga agcgtttggt caggagcagg aatttctcct gcaatccggc
     4501 tcggtccaga gggcgcgcgg gcgtgccgga aaactcggtc accgtgcggc tcacgctgcg
     4561 gccatccttc aatgtcactg tgacaagact ggtcaaatcc tcccgttcct gtcctggcgc
     4621 tgtcagcatc ttcacccgcg ccgccatggc gagaatcgcg ggatcgcggt ggacgttgtc
     4681 gtcgaatgaa tctggatcga tcggattgcg gtagagcgaa agggcgactg agaacggaat
     4741 gctgtattgg gccagcagca cgtcgggggg cgcggggatg ttgtttgtgg ttgccatgcg
     4801 tcgattgccg gtgacggtga tggccaggac atcgccggcg gagaatttgt attcgctcat
     4861 cagatcgagc atcgcttcga ccgggcggtg cgcggtgatg tggcaggcga aacgcttcat
     4921 catgatgtcc atcgaggcat aatcccggcc cagtcccttg gtgagttctg tgctgtcatg
     4981 ttcgttgcag aaggcgcgca gataacccgc ttcgccttcc agcacggtat cgggccccgt
     5041 gaaaccttcc gaagcgagac tggccgccag cacgcccgat tccgcggcgc ggccgagatg
     5101 caatttcttc accatgccgc cggtgttgga atgggcaaat tccagcaacc cgccagaaag
     5161 cgagccggca ataccgagtg cgtttgtcat cttcccggcg tccaatccca tcaattgacc
     5221 gaccgtcacc gcgccaccga acggccccgt cgtccccggc gcatgaaagc cgcgcggttc
     5281 gagcgagcct ttcgcggcac gcccgacgcg gatcatcacc tccgcaccgg ccgtcatggc
     5341 ggtgatgaga tcgcggccgc tcgacccgcg ttcctgggcg acggcaagac cggacgacaa
     5401 catgctggcg ccgggatggg agcccgagtc cggattggtc agattgtcga gctcgaaggc
     5461 atgggtcatc gtgccatggg ctagagccgc ggacggtgcc tgcaccatga cgccgccagt
     5521 gcccaggata tggctcttgc cgcccgggcc ggtgtgccgg gcatgggcta tgaccatctg
     5581 gctccagggc agtttgccgc catagaggat gacggccacc gtatcggtga tgcagtcctt
     5641 gatccgctgg cgtacttcgg ctggcaccgt ttcgtaacgc aatcccgccg cataggcggc
     5701 gagcttcggg gtgtaaccgc gttcgccctt tggcgcggct gcttctgccg ctgtcgcgga
     5761 ccccgtgcca agcaggggcg gagcgatcgc gccggcgcct gcggcaagcg ccacgcggtt
     5821 gagaaaactg cgccggccaa attgagttgg gtcctgctcg gccatgattg cctcccgctg
     5881 ggcgggccat gcccgcctct gttccccgca aactaaccaa aatcgcaggg aaagccgaga
     5941 gcaagacaaa cgcggaacgc aacggcgcaa cggggcgttc caggggactc ttcttagagg
     6001 accgtgcaca tgaatcagct ctccgccgaa acccgtcatg gcatcgagga agccttgcgt
     6061 tgccatggcg tctgtcttgc caccgccatg acgcactgcc tcgagatggg tggcgagcat
     6121 gtcaagccgc agcatttccg gctgatgatg gattgcgcgg ccatgtgcca attggccgcc
     6181 gatctgatgg cgcataagag ccagttccat cgccagtcct gcgcgttctg tgcggatatc
     6241 tgcgaggtct gcgcgcgaga ctgcgagcat atggagggaa tggaagactg tgccgccgcg
     6301 tgccgctcgt gtgcgcaatc ctgccgtgcg atggtgtagc gcacgcgaac gaattttccg
     6361 aaccggatgg atcaagctcc gaccccgcct gaaccgatgc gcaccggctg ctgtatcgcg
     6421 ggcggcggtc cggcgggcat ggtgctcggc tatttgctcg cgcgcgccgg cgtggaggtg
     6481 atcgttctcg agaagcacag gaattttctg cgcgattttc gcggcgacac ggtgcacccc
     6541 tcgactctcg agatcatgta cgaactcggt ctgctcgaag cgtttctcaa gcgtccgcat
     6601 caggagttgc gtgagctttc cgcgatcatc ggcggcgtga cggtaaggat tgccgatttc
     6661 tcgcacgtcc cggcgcattg caaattcatc gcgctgatgc cgcaatggga ttttctcaat
     6721 ttcctggcga gcgaggcgaa gcgctttgcc aatttccacc tgctgatgca ggccaaggtc
     6781 accgatcttc tgatggaagg cacgcggatt gccggcgttg ccgccaagac gccggagggg
     6841 cttcttcaga tacgtgccga tcttgtcgtc ggcgccgatg gacgccattc catcgtgcgc
     6901 gacaaggcag gtctgccacg cagggattat ggggttccaa tcgatgtctt gtggatgcgt
     6961 ctgtccaaaa acccaggcga tccagccacc acgctcggcc gcatcacggc gggcgccatt
     7021 ctagtaacgc tggatcgcgg cgactattgg caatgcgcct ttgtcatccc aaagggtgag
     7081 acgcagaacc ttcggatgcg cggtctcgat gcgtttcgtg gattgattgc gcgggtggcc
     7141 ccggagcttg gaaatcgcgt cgcggaattg accgattgga atcagatcaa gcttttgacg
     7201 gtcactgtgg accgcctcaa ggaatggtgt cgcccgggac ttctctgtat cggagatagc
     7261 gcccatgcca tgtcgcccat cggcggcgtc ggtatcaatc tggccgtgca ggatgccgtc
     7321 gccgccgcca atatcctggc ggtgccgtta cgaagcggca caccgccgct cagcgttcta
//
LOCUS       DASYEG010000321         4926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1436239, whole genome shotgun sequence.
ACCESSION   DASYEG010000321 DASYEG010000000
VERSION     DASYEG010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436239"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="VGM72_13580"
     CDS             complement(<1..1048)
                     /locus_tag="VGM72_13580"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4266344.1"
                     /translation="MNQEKLFIKCAWRLIPFLTLLYVVNFLDRVNVGFAALTMNKDLD
                     FSPAVFGFGAGVFFVGYALFQVPANIMLERIGARKWIFSIMAVWGALSALNAFVRSAH
                     DFYLLRFVLGAVEAGFVPGIFFYLTLWFPPAYRARFIASFMAAAPISVIVGGPLSGAV
                     LAMDGAFGLRGWQWLFLIEGLPACMLALAVLNVLPDGPASAAWLSPKEKALIVRCVDV
                     ENPVEHRDLWRALRDRRVIALGFVNFGVLAGANGVALWLPQIVHQMGFSNFLTGFIVA
                     LPSLASTIVMILWGRSSDARGERVWHVAIPALVAASSFLAGSLTQIDFLMLIALCVTQ
                     IMLWSAIAPLIAMPS"
     gene            complement(1418..2959)
                     /locus_tag="VGM72_13585"
     CDS             complement(1418..2959)
                     /locus_tag="VGM72_13585"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HEY4266345.1"
                     /translation="MIDRRRFLLTGLSWALVAAAPASFAAQSGPAFGPAQPFSFDRLS
                     RLAKMKAGEAYRPPAAPAADILNRLDIDTVQKIRFRPEYQLWREMPSRLPISFFHLNK
                     YAVEPVRIYAVSGGMAREIIYGPQYFDYGGTGLDMRLPANAGFSGFRVMNGQASAVDW
                     LAFQGASYFRSSGTQDQYGASARGIAINTALSTTEEFPRFSDFWLEEQGETVRIYALL
                     DGPSITGAYRFDAVKTSTVVMQVHAELFARADITRLGIAPLTSMYWYGENSHASSVDW
                     RPEVHDSDGLSMWTGTGERIWRPLNNPRTLQTNSFADKSPKGFGLMQRDRDFENYQDD
                     GAFYNKRPGIWVEPRGDWGEGAVQLVEIPTDDETHDNIVAYWQPKSSVTAGNSFKLDY
                     KLYWQDREPNAATMLARVVATRRGRGGIPGVNPPPTGKQKFVIDFEGGPLAQMEARYD
                     VSPVVTASRGKIDNAYVIKVVGTDRWRAFFDLTADGNAVVNLRCFLRLNGKALSETWL
                     YQYIA"
     gene            3106..3468
                     /locus_tag="VGM72_13590"
     CDS             3106..3468
                     /locus_tag="VGM72_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266346.1"
                     /translation="MLQCKIERHFFAAIAQQLRIISNGVIMTQRLVSFSFSPRAKTAI
                     DGGSYFRVSADGVGDDAPALAVALYSVPIPRFTPMPEQDLPIWTIAGLFRVLARTIAD
                     AVRPTELRTISIRENDYD"
     gene            3461..>4926
                     /gene="mdoH"
                     /locus_tag="VGM72_13595"
     CDS             3461..>4926
                     /gene="mdoH"
                     /locus_tag="VGM72_13595"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003962.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HEY4266347.1"
                     /translation="MTDIVVRRWLFLGLVTSSTVVADQPVWSVVRVDGVTPLEMMLFA
                     LFTILFAWIAASFWLACIGAYVRWQNLDVMIPVADSRREPSSHRPSRTAIVMPVFNED
                     ARRIFAGLAAVRDSVAAANGTGFDYFVLSDTNKTALQKVEEAEWRRLRDREPAVQNVF
                     YRHRLRNTGRKSGNIQDFCENWGVHYDYMVILDADSLMTGETLVRLVGLMDQNPRAAL
                     IQVPPQLIGRESLFARIQQFSSSVYGPCHAAGLAFLQGTDGNYWGHNAIIRVRAFMRH
                     CGLPKLPGRAPLGGEILSHDFVEAALLKRAGWDVLMAPALGGSYEEPPPTIFDYLKRD
                     RRWCQGNLQHARLILAIGLRMPSRLHFVMGVMNYLASPLWLLLLIASGVEMYFSAQVV
                     PVTYFGRYPILAAPVSHTVELLILLAFTLAILYAPKVLALCVASRDADTRLSHGGRGR
                     LARSVLLECLFSTFLAPVVMLSHSWFVLSILCGRTTGWG"
BASE COUNT         1000 a         1456 c         1455 g         1015 t
ORIGIN      
        1 tggacggcat cgcgatcagc ggcgcgatgg cggaccacaa catgatctgc gtcacgcaaa
       61 gcgcgatcag catgaggaaa tcgatttgcg tgaggcttcc ggcgaggaag ctcgatgcgg
      121 cgaccaaggc gggaatcgcc acatgccaga cacgttcgcc ccttgcgtcg ctggagcgcc
      181 cccagaggat catcacgatg gtactggcca aagacggcaa ggctacgatg aacccggtca
      241 agaaattgga gaagcccatc tgatgcacga tctgcggcag ccaaagtgcc acaccattgg
      301 cgccggcgag cacgccgaaa ttgacaaaac cgagtgcgat gacgcgccga tcgcgcaagg
      361 cacgccaaag atcgcgatgc tccacggggt tttccacgtc gacgcaccgg acaatcagcg
      421 ctttctcctt tgggctgagc caggcggcgg atgccggccc atcgggcaag acattgagca
      481 cggccagcgc cagcatgcat gcgggcagtc cttcgatcag aaacaaccat tgccatcccc
      541 gcaaaccaaa cgcgccgtcc atcgccagaa ccgcgccgga aagcggaccg ccgacgataa
      601 cggaaatcgg cgccgcggcc atgaagctcg caatgaaacg tgcgcgatag gcggggggaa
      661 accatagggt gaggtagaaa aatatcccag gaacgaaccc tgcctcgacc gcgcccagaa
      721 caaaccgcag aaggtagaaa tcatgggcgc tccgcacgaa cgcattgagc gccgacaatg
      781 cgccccagac ggccatgatc gagaagatcc atttccgcgc accgatgcgt tcgagcatga
      841 tgtttgcggg cacctgaaag agtgcgtaac cgacaaaaaa gacacccgcg ccaaagccga
      901 agaccgctgg cgagaagtcc aaatccttgt tcatggtgag agcggcaaag ccgacattga
      961 cgcgatcgag aaagttcacc acgtaaagaa gcgtcaggaa cgggataagc cgccaggcgc
     1021 atttgatgaa gagtttttcc tggttcatgc agttccactg cccgcacaga tcggcgcggc
     1081 agcggggcct ctccccttat tttgcaacac tatggcatgc gcgctcggtt cttggcagtg
     1141 ggaccgccga tcgttctgtg gtggcgaacc ggcatggcgt agccatggcg tcgtagtcgc
     1201 gggcgatttt tgcgatgcgc tctcggcctt tttcgggcgg ttacggcccg caggatgggt
     1261 tgaaaattct tcgtcgtgtg cgtggtaatt ttggacagga gattttgcgt caagccaatc
     1321 ggcatgggca gcgatgtcgc gtgccttatt cgcccgttca cgtcccatgg aatataaggc
     1381 agaactgcct ctggactaag acgaacgcat tgcggcgcta agcaatgtat tgatagagcc
     1441 atgtctcgga cagcgccttg ccattgaggc ggagaaagca gcgcaaattc accacggcgt
     1501 ttccatcggc ggtcaagtcg aagaacgcac gccagcgatc tgtccccacc accttgatta
     1561 cgtaagcatt atcgattttg cctcgcgatg cggtcacgac gggagaaaca tcgtatcgtg
     1621 cttccatctg cgccagcgga ccgccctcaa aatcgatgac gaatttttgt tttccggtgg
     1681 ggggcggatt cacgccggga atgccgccgc ggccgcggcg cgtggcgacg acgcgcgcca
     1741 gcatagtggc cgcgttgggc tcgcggtcct gccaatacag cttgtagtca agcttgaacg
     1801 aatttcccgc ggtcacggac gatttaggct gccaataggc aacgatattg tcatgcgtct
     1861 cgtcgtcggt ggggatttcg acgagctgca ccgcgccctc gccccaatcg ccgcgaggct
     1921 caacccatat gccgggacgt ttgttataga atgcaccgtc atcctggtaa ttctcgaaat
     1981 cgcgatcgcg ttgcatcaag ccgaatccct tgggactttt gtccgcaaag gagttcgtct
     2041 ggagcgtccg cgggttgtta agcggcctcc agatgcgttc tccggtgccc gtccacatag
     2101 acagcccatc gctgtcatgg acctccggac gccaatccac cgatgaggcg tgagaattct
     2161 caccgtacca atacatgctc gtaagcggtg cgatgccgag ccgtgtgatg tcggcacgcg
     2221 caaacaattc cgcatggacc tgcatcacga cggtgctggt cttcaccgca tcgaacctgt
     2281 aggccccggt gatgctgggc ccatcgagaa gcgcgtaaat cctgaccgtc tcaccctgtt
     2341 cttccagcca gaaatcggag aagcggggaa attcctccgt cgtcgacagc gcggtgttga
     2401 tggcgatgcc gcgcgccgag gcgccatatt ggtcctgggt ccctgacgag cgaaaatagc
     2461 tggccccctg aaatgcgagc caatcgacag cactcgcctg gccattcatc acccgaaatc
     2521 cggaaaaccc cgcattggcg ggcagacgca tgtcgagtcc cgtaccccca taatcgaaat
     2581 attgcggccc gtagatgatt tcgcgcgcca tgccccccga gacggcgtaa atgcggacgg
     2641 gctcgacggc atatttgttg aggtggaaga aagagatcgg cagccgcgat ggcatctccc
     2701 gccaaagctg gtattcgggg cgaaagcgaa tcttctgaac cgtatcgata tcgagtcggt
     2761 tgagaatgtc ggcagcagga gcagccggag gccggtaggc ctcccccgct ttcatctttg
     2821 caaggcgtga aagccggtcg aagctgaaag gttgcgccgg cccgaatgcc ggtcccgact
     2881 gagcggcgaa agaagcgggc gctgcggcaa cgagcgccca agaaagtccg gtcaggagaa
     2941 atctgcggcg atcgatcatg cgggtcccgg ttctatgacg tctcggtggc cgctcctgta
     3001 acctaaccaa gacatttcta agaccataag aactctttca aattcaggac gtttcccgcg
     3061 cacccatgaa tcgtgacctt tttctgattt agtagactac gattcatgct gcagtgcaaa
     3121 atcgagcgtc atttttttgc tgcaattgcg cagcagcttc ggattatcag taatggagtt
     3181 atcatgactc agcgtcttgt ttccttttcg ttctcaccac gtgcgaaaac cgctatcgac
     3241 ggcggatcct atttccgggt ttcggccgat ggagtgggag atgacgctcc agcgttagcc
     3301 gtcgctctct attccgtacc gatcccgcga ttcaccccga tgccggagca ggatctcccg
     3361 atctggacta ttgccggcct gttcagggtg cttgcgcgta caattgccga tgcggtgaga
     3421 cccaccgagt tgcgcaccat ttccattcgc gaaaacgatt atgactgaca tcgtcgtaag
     3481 gcgatggctt tttctcggtt tggtgacgtc atcgacggtg gtggccgacc agccggtatg
     3541 gtctgtcgtc agagtggacg gcgttacccc gttggagatg atgctcttcg cgcttttcac
     3601 gatcctcttt gcatggatcg cggcgtcctt ttggctcgcc tgcattgggg cctatgtgcg
     3661 ctggcagaat ctcgacgtca tgatcccggt ggcagattcc cgccgcgagc cgtcgtctca
     3721 ccgcccctcg cgcaccgcca ttgtcatgcc ggttttcaat gaggacgccc ggcgtatctt
     3781 cgccggcctc gcggcagttc gcgattcggt ggccgcggcg aatggaacgg gtttcgatta
     3841 tttcgtcctg agcgacacca acaagacggc gttgcaaaag gtagaagaag ccgaatggcg
     3901 ccgcctgcgc gatcgggaac cggcggtgca gaatgtcttt tacaggcatc ggttgcgcaa
     3961 taccggccgc aagagcggga acatccagga tttctgcgaa aactggggag ttcactacga
     4021 ttatatggtg atcctggacg ccgacagcct gatgaccggc gagacgctgg tccggcttgt
     4081 cggcttgatg gaccagaatc cgcgcgcggc tctcattcag gttcctccgc aactcatcgg
     4141 gcgcgaatcg ctgttcgcgc gcatccagca attctcctcg agcgtctatg gtccctgcca
     4201 cgccgcggga ctggcgttcc tccagggaac cgacggaaat tattggggcc ataacgccat
     4261 catccgggtg cgcgctttca tgcgccactg tggcctgccg aagcttcccg gacgcgcgcc
     4321 gctgggcggg gaaatcctca gtcacgattt cgtggaagcg gcccttctca aacgcgccgg
     4381 ctgggatgtg ctgatggcac cggcgctcgg gggctcctat gaagagccgc cgccgacaat
     4441 ctttgattac ctgaagcgtg accgtaggtg gtgccagggc aatttgcagc acgcccggct
     4501 gatactcgcc atcggattgc gcatgccgag ccggcttcat ttcgtcatgg gcgtgatgaa
     4561 ttatctggct tcgccgctct ggctcctgct gctgatcgct tccggcgtgg agatgtattt
     4621 ttcggcgcag gtcgttccgg tgacctattt cggacgttat ccgatcctgg ccgcgcccgt
     4681 ctcgcacaca gtggaactgc tcatcctcct cgccttcacg ctggcgattc tctatgcgcc
     4741 caaagtgctt gctctctgcg tcgcctcgcg ggacgccgat acgcgccttt cgcatggcgg
     4801 cagaggcagg ctcgcgcgca gcgtcctgct cgaatgcctc ttttccacct tcctggcgcc
     4861 ggtggtgatg ctttcgcata gctggtttgt gctgagcatt ctgtgtggcc gaaccacggg
     4921 ctgggg
//
LOCUS       DASYEG010000322         5394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3462625, whole genome shotgun sequence.
ACCESSION   DASYEG010000322 DASYEG010000000
VERSION     DASYEG010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3462625"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VGM72_13600"
     CDS             <1..553
                     /locus_tag="VGM72_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266348.1"
                     /translation="NAFNRRYYGRYGNDPNNFARDRARFRAQQQWHERYPHDYVYADD
                     AFYRDCQTNNEVGGAIVGAILGGLLGNAASNGQGGATLAGIILGGAGGASIAGNLDCQ
                     DRSYLYRSYYDGFERGRPQASYPWRNDRTGDYGTLLVGDYYRDRDNYRCATYTQTIYV
                     RGRPQTGRGHACRQEDGTWALVD"
     gene            complement(609..1073)
                     /locus_tag="VGM72_13605"
     CDS             complement(609..1073)
                     /locus_tag="VGM72_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266349.1"
                     /translation="MNAKTAWLIGTGVLSLSVLMSPAQSADNAAAQKHKPVTITRLYT
                     GPDGQTHAEEIEAKFTPAGGNDVYKLMANSGAELHRAPPGRVADWHTAPRRQYVMTIS
                     GRGEIELMGGKKIEVGPGNIELAEDLTGKGHITRTVGNEDRVTIQIPLSDQK"
     gene            1201..2130
                     /locus_tag="VGM72_13610"
     CDS             1201..2130
                     /locus_tag="VGM72_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266350.1"
                     /translation="MTRRVPEFIAAGLIGAVVLGAGSAATGAQERASIPDFTGFWGRN
                     AFDPEFLPSGPNPVANMNRKDGDTMHPSLGGDPLPLVGDYNNPILKPEAAAVVKQTGE
                     IAAGGKIYPDPSNQCAPYSPPYIFTLQLGLQMLQGKDHITMLYQQDDQVRRVRLNASH
                     PKTVKPSAMGDSVGHYEGDTLVIDTVGVKIGPVTMVDRYGTPQSAALHLVERYRLIDV
                     AEAKAAMDRHEKRAGRVGGPPGAIPMDPEYGKALQLQYTVEDPNVFTMPWSAQITYRL
                     SPLAWQEQICAENIVEYWPGMNKGVPRADRPDF"
     gene            complement(2159..2629)
                     /locus_tag="VGM72_13615"
     CDS             complement(2159..2629)
                     /locus_tag="VGM72_13615"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY4266351.1"
                     /translation="MTPLDSIDIRILALLQRDASCSISEIGDAVHLSQNACWRRIKRL
                     EDEGVIKKRVALLDPQKLDVGVTVFVSVRVGEHSEKWLETFTALVRKMPEVIEFYRMA
                     GDVDYLLKLQVADIPAYDRVYKSLIHAAKLMDVSAAFAMEEMKRTTELPLPRPD"
     gene            2726..2977
                     /locus_tag="VGM72_13620"
     CDS             2726..2977
                     /locus_tag="VGM72_13620"
                     /inference="COORDINATES: protein motif:HMM:NF040386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6356 family protein"
                     /protein_id="HEY4266352.1"
                     /translation="MLQRWFLDHPHSVGESYFEHQRTAFGFSATLFMAAAACLVHGLI
                     PGLLTRTASGKIAQLHERMSIHRRRGERLASGAPDGVNG"
     gene            3104..4150
                     /locus_tag="VGM72_13625"
     CDS             3104..4150
                     /locus_tag="VGM72_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEY4266353.1"
                     /translation="MNPNRHRESWTASALRFHSEIRRLVPGLALAGGIAILALLASSV
                     LFSGIVSPLFIAMGLGIVVRNLVGTPGAALPGLKFGLRRILRLAVMLLGLQLTIAQIA
                     EVRWAGLLVIALTLVATFLFTIWAGRALGVERKLTELIASGTSICGASAIIAVNTVIQ
                     ASDEDVSYAIACVTVFGSIAMFVYPLLPGLLHLAPHAYGLWTGASIHEVAQVIAAAFQ
                     DGETAGHIGTLAKLSRVIMLAPAVVTLGILASRRSRSESGEAGTTGTPLPYFVLGFVA
                     LVGLNSVMVFPPDVSRWTIAFTAFLLSLSLAAMGLETDVRKLRARGLRPVVLGASAGL
                     FIAAFSLTLIKFTS"
     gene            4312..4959
                     /locus_tag="VGM72_13630"
     CDS             4312..4959
                     /locus_tag="VGM72_13630"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY4266354.1"
                     /translation="MKRLPLSVLAILVLVAFVAGGALWHLGDLRAQSGTAPPASPRGE
                     LGGPFSLIDQYGMRRTDMDFRGKYMLVFFGYTYCPDVCPTTLAVEAEALDKLGSRADG
                     IVPIFISVDPKRDTPGKLKSYLASFDAKPPSVRPSFVGLTGNGEEIAAAAKAYRVYYR
                     AHLDTRTDDGANYSIDHSSDVYLMSPEGKFTAYYSQGILPDELAADLMQKTLSPR"
     gene            5100..5378
                     /locus_tag="VGM72_13635"
     CDS             5100..5378
                     /locus_tag="VGM72_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266355.1"
                     /translation="MKALRSTIALIALSIPSSIAAQTSPSAAGTYVITQGTAGNTPVT
                     ILLDSVNGKTWILGQVDEAGRTLPAGQTGSATWIPLSFAAKVPPSPPR"
BASE COUNT          977 a         1608 c         1653 g         1156 t
ORIGIN      
        1 gaacgcgttc aaccgccgct attatggccg ttacggaaac gaccccaaca atttcgcgcg
       61 cgaccgggca cggttccggg ctcagcagca atggcacgag cgctatcctc atgattatgt
      121 gtatgcggat gatgcgttct atcgcgattg ccagacgaac aacgaggtgg gcggtgcgat
      181 tgtcggcgcg atcctgggcg gccttttagg caacgcggca tcgaacgggc agggtggcgc
      241 cacgcttgcg ggcatcatcc tgggcggggc cggcggcgcc agtatcgcgg ggaatctcga
      301 ttgccaggac cgttcctacc tgtatcgcag ttattacgat ggcttcgagc gcgggcggcc
      361 gcaggcgagc tatccctggc gcaacgacag aaccggagat tacggcacgc tgctcgtcgg
      421 cgattattat cgcgaccggg acaattatcg ctgcgcgacc tacacccaaa ccatctatgt
      481 gcgcggcagg ccgcaaaccg gccgtggcca tgcctgccgg caggaggatg gaacctgggc
      541 gcttgtcgac tgacccgcgc gtaacaggct cggcggtgac gccggccggt acgccttggt
      601 tatgggatct atttttggtc cgagagcgga atctgaatgg tcacccgatc ttcgtttccc
      661 accgttcggg tgatatggcc tttgccggta aggtcttcgg ccagttcgat gttgcctgga
      721 ccgacttcga tcttctttcc gcccatcagc tcgatctcgc cacgaccgct gatcgtcatc
      781 acatattgcc ggcgaggcgc cgtatgccag tcggcgacac ggcccggagg cgcgcggtga
      841 agctccgcgc cggaattggc catcagctta tagacgtcat tgccgcccgc gggtgtgaac
      901 ttggcctcga tctcctcggc gtgcgtctgt ccatcgggcc cggtgtatag ccgcgtgatc
      961 gtgacgggct tgtgcttttg ggccgccgca ttgtccgcac tctgcgccgg tgacatcaga
     1021 acggacagac tcaacacacc ggtaccgatc aaccacgcgg tcttcgcatt catgtgggcc
     1081 tcctgctgtg tccaagccga agcatgaccg atcgatggga aaagtccatc gcggatgccg
     1141 gccacggctt ggagcggcgg ggccgatatg ctgatatgct ggcggagcag gggggctcgc
     1201 atgacgcgcc gggttccgga attcatcgcc gcggggttga tcggtgctgt cgtgctgggc
     1261 gcgggttctg cggccacggg cgcacaggaa cgcgcctcca tccccgattt caccggcttc
     1321 tgggggcgta acgcgttcga tcccgaattc ctgccttccg gtccgaatcc cgtggcaaat
     1381 atgaatcgca aggacgggga cacgatgcac cccagccttg gcggcgatcc tctgccgctt
     1441 gtcggcgatt acaacaatcc gatcctgaag cccgaagccg ccgccgtggt gaagcaaacc
     1501 ggcgagattg cggcgggcgg caaaatctat cccgacccct ccaaccaatg cgcgccctat
     1561 tcgccgcctt atatcttcac gctccagctc ggtctgcaga tgcttcaggg caaggaccac
     1621 atcaccatgc tgtatcagca ggacgatcag gtccgccgcg tgcgcttgaa tgcgtcccat
     1681 cccaagaccg tcaaaccatc ggcgatgggc gattcggtgg ggcattatga aggcgatacg
     1741 ctcgtcatcg acacggtggg cgtaaaaatc ggaccggtga ccatggtcga ccgctacggc
     1801 acgccgcaaa gtgccgcgct ccatctggtc gaacgctatc gcctgatcga tgtcgccgaa
     1861 gcaaaagccg cgatggatcg gcatgaaaaa agagctggcc gcgtcggagg tccgccgggc
     1921 gcgattccga tggaccccga atatggcaag gcgcttcaac tgcaatatac ggtggaagac
     1981 ccgaacgtct tcaccatgcc gtggtcggcg cagatcacct atcggctcag tccgcttgcc
     2041 tggcaggaac agatttgcgc cgagaacatc gtcgaatatt ggcccggcat gaacaaaggc
     2101 gtcccgcgcg cggatagacc cgatttttga accgtcttag acgcaggcat ggcgtgcttc
     2161 aatcgggcct gggaagcggg agttccgttg tgcgcttcat ttcttccatc gcgaaggccg
     2221 cgctgacatc catcagtttc gcggcgtgga tcagcgattt gtagacgcga tcgtaggctg
     2281 ggatgtctgc gacctgcaat ttgagaaggt aatccacatc gcctgccatc cggtagaact
     2341 cgatcacctc gggcattttg cgcaccagcg ccgtgaaggt ttccagccat ttttcgctgt
     2401 gctccccgac gcgtacgctc acgaaaaccg tgaccccgac gtcaagtttc tgcggatcga
     2461 gcagcgcgac gcgctttttg atgacgcctt cgtcttccag acgcttgatc cgccgccagc
     2521 acgcgttctg ggagagatgg accgcatcgc cgatctcgga aatggagcat gaagcgtcac
     2581 gctgcagcag ggccaggatt ctgatgtcga tggaatccag gggtgtcatc gaataatttc
     2641 ccaaatcagt gccaatttgg tggatagttt gggaagaaaa ttgacgaatc tgcaagtaaa
     2701 ttgatgcctc gtcagaggac gcatcatgct tcagcgctgg ttcctggatc acccccattc
     2761 ggtcggagag agctatttcg aacaccagcg cacggccttc ggcttttccg cgaccctgtt
     2821 catggccgcg gccgcgtgcc tcgttcacgg actgatcccc ggcctgctca cgcgcacagc
     2881 gagcggaaag attgcgcagt tgcacgagcg aatgagcatt catcgccggc gcggcgagag
     2941 gcttgcgtcc ggtgcgcccg atggcgtgaa cggttgacgg gttcatgacg gtgccatccg
     3001 cgcggatggc gttcttacct gactggtttt tgcctgactg gtttacctga ctggttttga
     3061 agagcgcatt gggggctgaa ttcttgcatt gcgatttgct gtgttgaacc cgaacaggca
     3121 ccgggagtcc tggaccgcga gcgcattgcg attccacagc gaaatccggc ggctcgttcc
     3181 cggattggct ttggcggggg ggatcgcgat tcttgcgctc ctggccagca gcgttctgtt
     3241 ctccgggatc gtcagcccgt tgttcatcgc gatggggctg gggattgttg ttcggaacct
     3301 cgttggtacg cctggcgccg cactccccgg tttgaagttc ggtctccggc ggattctgcg
     3361 tttggccgtg atgcttcttg gtctccaact caccattgcg cagatcgcgg aagtccgatg
     3421 ggccggcctc ttggtcatcg ccctaacgct cgtagcgacc tttcttttca ccatttgggc
     3481 ggggcgagcg ctcggggtgg aacgcaagct gaccgaattg atcgcctcgg gaacatccat
     3541 ctgcggcgct tcggcgatta tcgcggtcaa cacggtgatc caggcatcgg acgaagatgt
     3601 cagctatgcc atcgcatgcg tcaccgtttt cggatcgatc gccatgtttg tgtacccgtt
     3661 gctgccggga ttgcttcatc tcgccccgca tgcctatggc ctgtggaccg gagcctcgat
     3721 ccacgaggtc gcgcaagtca tagcggcggc ctttcaggat ggagagaccg ccgggcatat
     3781 agggacgctc gcgaagctca gccgtgtgat catgctggcc cctgccgtag tgacgcttgg
     3841 catcctcgcc tcccggcgga gccggagtga atcgggtgaa gccggtacta ccggcacgcc
     3901 gctgccgtat ttcgtgcttg gcttcgttgc ccttgtgggc ctgaacagcg tcatggtgtt
     3961 tccgccggat gtcagcagat ggacgatagc gttcacggca tttcttcttt ctctttcgct
     4021 cgcggcaatg ggccttgaga ccgatgtgcg caaactgagg gcgcggggcc tgaggccggt
     4081 cgtgctgggg gcttcggccg gattgttcat cgcggctttc agcctgacat tgataaaatt
     4141 caccagttga gcggcgcctc gaacgccgtc tctatgtgga tggctccaag caggggtgtc
     4201 tgcaacgcct atggcaggtg aaggttttgc ctccccgcgt cgccgggcaa catgctattg
     4261 ttccccccgg aatccattgt catctggcca ttgtcatccg gggaatattg aatgaaacgc
     4321 cttcccctga gcgttctggc aattctcgtt cttgtggctt tcgtggccgg gggcgcgctt
     4381 tggcacttgg gcgatctgcg cgcgcaaagc ggaacggcgc cgccggcctc tccgaggggc
     4441 gagttgggcg gtccattttc gctcatcgat caatatggca tgcgccgcac ggacatggat
     4501 ttccgcggaa aatatatgct ggtattcttc ggctacacgt attgtccgga tgtttgtccg
     4561 acaacccttg cggtggaagc agaggcgctc gacaagctcg gctcgcgtgc cgatggcatc
     4621 gtaccgattt tcatttccgt cgatcccaag cgggacacac cgggcaaact caaatcctat
     4681 cttgcctcgt tcgacgcgaa gccgccctcc gtgcgcccca gcttcgtcgg cttgacgggt
     4741 aacggcgaag agattgccgc agcggccaag gcctaccgcg tctattaccg cgcgcacctg
     4801 gatacgcgca cggacgacgg agcgaactac tccatcgatc attcgagcga cgtctatctc
     4861 atgagtccgg aaggaaagtt cacggcctat tattcacagg gaatcttgcc cgacgagctg
     4921 gccgccgatt tgatgcagaa gacgctctcg ccccgctgac tcgcggttcg atatcctgcc
     4981 cacgagtgga cggcccacga atggacggcg atgccggcat tgcgcgaggg ctataccttt
     5041 gctgtcctgc agaggcgcgt tatgacaggc cacgtggcgg aacatcgcgg gagcggagca
     5101 tgaaagcatt acggtctacg atcgcattga ttgcactttc catcccaagc tcgattgccg
     5161 cgcaaacctc gccaagtgcg gcgggcactt acgtcattac ccaagggacg gccggcaata
     5221 cgccggtgac gattttactg gattcggtca acggcaagac ttggattttg ggacaggtgg
     5281 atgaggcggg ccgtacgctt cccgccggtc aaaccggctc ggcgacatgg attccgcttt
     5341 cgtttgccgc gaaggtgccg ccgtcgcctc cgcgataatt gcactcggcc gtta
//
LOCUS       DASYEG010000323         2445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2593205, whole genome shotgun sequence.
ACCESSION   DASYEG010000323 DASYEG010000000
VERSION     DASYEG010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2593205"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..940)
                     /locus_tag="VGM72_13640"
     CDS             complement(125..940)
                     /locus_tag="VGM72_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266356.1"
                     /translation="MTAFICTACGTQYPPSEKPPAECPICEEERQYVPPRGQSWTTLE
                     GLAQSHMNSFCEYEPGVVGIGAGFAIGQRAILVRTPGGNVLWDCISTLNDATVTMIKG
                     LGGIQAIAISHPHFYTAMGAWSRAFDDAPIHLSAADKDWIMNPSPAVKLWQGDTLKLW
                     DGVTLVRCGGHFPGGTVMHYAGGAGGKGIVCSGDILTVTTDRKWLSFMRSYPNLIPLS
                     APEVEHIGVAMKPFAFEAIYGHYFDRVIPKDAKSVLEKSVERYVGAINGTRGY"
     gene            1032..1328
                     /locus_tag="VGM72_13645"
     CDS             1032..1328
                     /locus_tag="VGM72_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266357.1"
                     /translation="MKHTWNNPWTPLRIAVLAAAAFGTLFWLGSIVQWWRIANTRRDG
                     FELMGLMLSTGFFVALVLPTLTLGLIGRWLGLGAILGLAVVVLATDTVWPWLPW"
     gene            1357..1950
                     /locus_tag="VGM72_13650"
     CDS             1357..1950
                     /locus_tag="VGM72_13650"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEY4266358.1"
                     /translation="MLHKATPERLSAFSDGVFAVLITILVLELHPPEMPTFKALLSLW
                     HTWLSYAVSYLFIAIVWINHHHLMRYATEATSRLLWFNFAHLFSVSLLPLATAWMAVS
                     KLDPQPVAFYALVFFLVNATYICLIWELVDRSQTRSVPQRERKIMRVRAIVTLCFFGA
                     AVAVALKYPLIGLGICICCLIVYLKPAPPAVRDKSSG"
BASE COUNT          409 a          778 c          735 g          523 t
ORIGIN      
        1 tcccggccat ttttttagac cctcatggtg aggagcgcgt agcgcgtctc gaaccatgag
       61 agcccggccg tggccgcctc gtccttcgag acgccgctac gcggctcctc aggatgaggc
      121 ggagttagta cccgcgcgtg ccgttgatcg cgcccacata acgctcgacc gatttctcca
      181 gcaccgattt cgcatccttc ggaatgacgc ggtcgaagta gtggccgtag attgcctcga
      241 aggcgaatgg cttcatcgcg acgccgatgt gctcgacttc cggcgcggag agcgggatca
      301 agtttggata gctgcgcatg aacgacagcc atttgcggtc tgtcgtgacg gttagaatat
      361 cacccgagca gacaatgccc ttgccgcccg ccccgcccgc gtaatgcatc accgtgccgc
      421 cggggaaatg gccgccgcag cggaccagcg tgacgccatc ccataacttc agcgtatcgc
      481 cctgccacag cttcaccgcc ggtgacggat tcatgatcca gtctttatcc gcggcgctga
      541 gatggattgg cgcatcgtca aaggcgcggc tccacgcacc catcgccgtg tagaaatgcg
      601 gatgtgaaat cgcgatcgcc tggatgccgc cgaggccctt gatcatcgtc acggtcgcgt
      661 cgttgagcgt cgatatgcaa tcccacaaca cgttgccgcc gggcgtgcgc accaatatcg
      721 cgcgctggcc gatggcgaag cccgcgccga tgccgacgac gcccggctcg tattcgcaaa
      781 agctgttcat gtggctctgc gcgagaccct cgagcgtggt ccaactctgg ccgcgcggcg
      841 gcacgtattg ccgctcctcc tcgcagatcg gacattcggc aggcggtttt tcggacggtg
      901 gatattgagt cccgcaagct gtgcagatga aggcggtcat tgcaggcgtt tccccggggt
      961 tcttttgaac tgcttattgt gtcaccgccg catcattgct gttagccttg gccaaggcaa
     1021 tcgggggccg catgaaacat acttggaata atccttggac gccgctgcgc attgcggtgc
     1081 tagcggctgc cgcattcggc acgctgttct ggctcggctc aatcgtgcag tggtggagga
     1141 tcgcgaatac acgccgtgat ggcttcgagc tgatgggcct gatgctgtcg accggatttt
     1201 ttgttgcgct ggtactaccg acgttgacgc ttggcctcat cggccgctgg ctcggcctcg
     1261 gcgccattct aggcttggcc gtggtggtgc tcgccaccga tactgtgtgg ccctggctgc
     1321 cgtggtagga acgatgaacc acccggagac gatgccatgc tgcacaaagc gacccccgag
     1381 cggctgagcg cattctccga cggcgtgttc gccgtgctga taacgatttt ggttctggag
     1441 ttgcatccgc cggagatgcc gaccttcaag gcgcttctgt cgctatggca cacatggctg
     1501 agctatgccg tcagctatct tttcatcgcc attgtctgga tcaatcacca tcatcttatg
     1561 cgctatgcca cagaggcgac gtctcgcctc ctgtggttca acttcgcgca tcttttttcc
     1621 gtgtcgttgc tgccgctcgc cacagcctgg atggcggtga gcaaactgga cccgcaaccg
     1681 gttgccttct atgcgctggt cttcttcctg gtcaacgcga cttacatctg cctgatctgg
     1741 gagctggtcg accgaagcca aacgcgcagc gttccgcaaa gagagcgcaa gatcatgcgc
     1801 gtacgggcga tcgtgacatt gtgctttttc ggcgctgccg tcgcggtcgc actgaaatat
     1861 ccgctgatag ggctcggaat ttgtatctgc tgcctgattg tttatctcaa gcccgcaccg
     1921 cctgcggtgc gcgataaatc ttccggctga gcgaacagca cgagcacgtc cctcacgatt
     1981 tgacggcatc acggtgtgtc tgccgcggac atgcggcatc acccgtattc tttgcggcgc
     2041 cgggtacgcc gtagtccccc tcatcgcgcc cctcaagaag tgaggggatg gagcgccggg
     2101 tgacgcacct tgtagtcagc cgccgcgcaa acaagcatgc gcagtttgcg ccgcttgact
     2161 gcgttttgtg gcggcggcgc gccttgcgat cggcacgctc gccacccggc gctccacttg
     2221 cggcgatttc tgtccctggg accgtaactt ccgggcgcgc gacggaggat ttctcctcac
     2281 gggcttccgc ccgccttcgt ccgcaccacg tccagccatc aaaggcagcc cctcgtcgtg
     2341 ggggcggacg gttgcccaag gcctcccgag tgccggcttg cgaggccggc ccgcgggcgc
     2401 cgcacactta aagccggttg cccggcccca agccggaaga gccct
//
LOCUS       DASYEG010000324         2813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2593212, whole genome shotgun sequence.
ACCESSION   DASYEG010000324 DASYEG010000000
VERSION     DASYEG010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2593212"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VGM72_13655"
     CDS             complement(<1..534)
                     /locus_tag="VGM72_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266359.1"
                     /translation="MKFGLIGLLMLAASPAALAAENPPEIPFDGDVNFLKMPKDMYLG
                     EVSGVAVNPQGHLFVFHRGNTNGPAYGGRAAQLLEFDQNGNFVREIGHNLYAWSFAHT
                     VRVDHDNNVWVADKGSDMVIKFQMPEGRPVMMLGRKPEAADEGAHPLEHPNPPLPALD
                     GLFRQVTDMTWDPAGNIY"
     gene            complement(638..979)
                     /locus_tag="VGM72_13660"
     CDS             complement(638..979)
                     /locus_tag="VGM72_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266360.1"
                     /translation="MRNAVRTALFAAVLCSAAAIGTPSFAQINAAHAKVAPDEKTAIA
                     LAEKAALPLIGGEDKLAIARPFRATGHGDVWLIISQPVPDPKNPAAPKRSVVVQLSAE
                     TGEIFTVDTAD"
     gene            complement(1070..2344)
                     /gene="eno"
                     /locus_tag="VGM72_13665"
     CDS             complement(1070..2344)
                     /gene="eno"
                     /locus_tag="VGM72_13665"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019837270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY4266361.1"
                     /translation="MTAIVDIVAREILDSRGNPTVEVDVRLEDGSAGRAAVPSGASTG
                     AHEAHELRDGGARYAGKGVRRAVDAVNGEIFDAIGGMDAEDQTRIDSLMCRLDGTVDK
                     SRLGANAILGVSLAVAKAAALSSCLPFYRYVGGAKAQVLPVPMMNIVNGGAHADNPID
                     FQEFMIMPVGAASFSEGLRAGAEIFQALKKQLKDAGHNTNVGDEGGFAPNLKNPDEAL
                     AFIMGAIEKAGYRPGEEVMLALDCASTEFFKNGKYNLEGEGKTLDADAMVKLYQDLCA
                     RYPIVSIEDGMAEDDWEGWAALTSALGGKIQLVGDDLFVTNTARLKDGIAKGIANAIL
                     VKVNQIGTLSETLDAVETAHRASYKAVMSHRSGETEDTIIADLAVATNCGQIKTGSLS
                     RSDRLAKYNQLLRIEEALGDSAIYAGRSVLRS"
     gene            2482..>2813
                     /locus_tag="VGM72_13670"
     CDS             2482..>2813
                     /locus_tag="VGM72_13670"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEY4266362.1"
                     /translation="MPLTYYLAAVDTILACLVTFAMGGWSGYLRSKYKIEAPSVVGHP
                     AFERAHRTHINTIENLVLMIPLLWVASVFYGGQIPFWLGLLWVVSRILYAIGYAQTNA
                     QLREPGAG"
BASE COUNT          547 a          870 c          834 g          562 t
ORIGIN      
        1 atagatattg ccggcggggt cccacgtcat gtccgtgacc tgacggaaca gcccgtcgag
       61 cgcgggcagc ggtggattgg gatgttcgag cggatgggcg ccttcgtccg cagcctcggg
      121 cttgcggccc agcatcatca cgggtctgcc ttccggcatc tggaacttga tcaccatgtc
      181 cgagcccttg tcggcaaccc aaacattgtt gtcgtggtcg acgcgcaccg tgtgcgcaaa
      241 ggaccaggcg tagagattgt gcccaatttc gcgcacgaaa ttgccgttct ggtcgaattc
      301 gagaagctgc gccgcgcgac cgccataggc gggcccattg gtgttgccgc gatgaaagac
      361 gaacagatgt ccctgcggat tgaccgcgac gcccgagact tcgcccaaat acatatcctt
      421 gggcattttg aggaaattga catccccatc gaacggaatc tcgggcgggt tttccgccgc
      481 caaggctgcc ggcgacgcgg caagcatgag caatccgatc agaccaaatt tcatgaatcc
      541 ctccctgagc cgatgcgtgc ccgcgaggac cgcaactgcg cgcgagcata gggctcgatc
      601 gaaaggatga caattgcgcg cccaagcgcg ctagagatca gtccgccgta tcgacggtga
      661 agatctcacc cgtctccgcg ctcaactgca caacaacgga gcgcttcggc gcggctggat
      721 tcttggggtc gggaaccggc tgcgagataa tcagccagac atcgccatga cctgtcgccc
      781 gaaagggccg tgcgatggcg agcttgtctt cgccgccgat caatggaagg gctgcttttt
      841 ccgccagcgc gattgccgtt ttctcatcgg gcgcgacctt ggcgtgggcg gcattgatct
      901 gcgcgaacga cggcgtgccg atggcggcag cggagcaaag aacggccgca aaaagcgcgg
      961 tgcgaacggc attcctcatc gacgactcca attgaaaagg ccttacgcgc cttatagtcc
     1021 attccctcaa cggaaatgaa ttctatcaca aggaatttac ctttgaaaat taactccgca
     1081 aaaccgagcg gcccgcgtaa atcgcagaat caccaagcgc ctcttcgatg cgcaagagct
     1141 gattgtattt cgccagccgg tcggagcgtg acagcgagcc ggtcttgatc tgcccgcaat
     1201 tggtggccac cgccaagtcc gcaattatgg tgtcctcggt ctcgcccgaa cgatgcgaca
     1261 tcaccgcctt gtaggaagcg cgatgggcgg tctccacggc atccagcgtc tcggacaacg
     1321 tcccgatctg gttgaccttc acgaggatcg cgttggcaat gcctttggcg atgccgtcct
     1381 tcagccgggc ggtgttggtg acgaagagat cgtcgcccac aagctgtatc ttgcctccca
     1441 gcgccgaggt cagcgcagcc caaccttccc aatcgtcttc ggccatgccg tcctcgatgg
     1501 aaacgatcgg atagcgcgcg cataaatcct ggtagagctt caccatcgcg tccgcgtcga
     1561 gggttttgcc ttccccttca agattgtatt tcccgttttt gaagaattcg gtcgaggcgc
     1621 aatcgagcgc gagcatcacc tcttcgcccg gccggtatcc ggctttttcg atcgcgccca
     1681 tgatgaaagc gagcgcttcg tcgggatttt tgagattggg cgcgaaaccg ccttcgtcgc
     1741 cgacattggt gttgtggccg gcatccttga gctgcttttt cagcgcctgg aagatttcgg
     1801 cgccggcacg caagccttcg gaaaagcttg ccgcgcccac cggcatgatc atgaattcct
     1861 gaaagtcgat cgggttgtcg gcatgggcgc cgccattgac gatattcatc atcggcaccg
     1921 gcaggacctg cgccttcgcg cctccgacgt agcggtagaa cggcagacac gacgacaagg
     1981 cggccgcttt tgcgacggca agcgagacgc cgagaatcgc attggcgccg aggcggcttt
     2041 tatccaccgt gccgtcgagg cggcacatca gagaatcgat tcgcgtctgg tcttcggcgt
     2101 ccatgccgcc aatggcgtcg aatatctcgc cgttcacggc atcgacggcc cggcgcacgc
     2161 ccttgcccgc ataacgcgcg cccccatcgc gcaattcatg tgcctcatga gcgcccgtag
     2221 aagcgccgga cgggacagcc gcgcggccgg cggagccatc ctccaggcgg acatcgacct
     2281 cgaccgtcgg gttgccgcgg ctgtcgagga tttcccgcgc gacgatatcg acgatggcgg
     2341 tcatgcaaag ctcccgtgac gagcggccct tctaggcgat ggccgttggc gcttcaagcc
     2401 gccggtagcg cttcaagcca agggggaccc gaagatgaaa aacaggttac gctcaccatg
     2461 agggctcatt cgagggaaaa catgccgctc acctattatc ttgccgccgt cgacaccatt
     2521 cttgcctgtc tcgtcacctt cgcgatggga ggctggagcg gctatctccg gagcaaatac
     2581 aaaatcgaag cgccttcggt tgtcggccac cccgccttcg agcgcgctca tcgcacgcat
     2641 attaacacga tcgagaatct cgtgctgatg atcccgctgc tgtgggtcgc gagtgtgttc
     2701 tatggcggcc agattccatt ttggctcggc ctcttgtggg tggtgagccg catcctctac
     2761 gcgatcggct atgcgcagac gaatgcgcaa ctccgcgagc cgggcgcggg att
//
LOCUS       DASYEG010000325        14529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2446963, whole genome shotgun sequence.
ACCESSION   DASYEG010000325 DASYEG010000000
VERSION     DASYEG010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 14529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14529
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2446963"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            398..1063
                     /locus_tag="VGM72_13675"
     CDS             398..1063
                     /locus_tag="VGM72_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266363.1"
                     /translation="MNKTILLSAVAMTLAGAGAVLAAQAPAQPMSFFITSTTPTGSGN
                     LGGLAGADKICQDLAAAAGAGGKTWHAYLSTQPANGMPGVNARGRIGAGPWYNAKGVM
                     IAANNGDLHGDNQRDRNNIQKANMLTEKGELIKGFGDMPNEHDILTGSDSDGRAFPAG
                     LDTTCNNWTSDGADHKAMLGHADRSGGGNVSWNSVHMSADCTKAGLIRTGGAGHLYCF
                     AIN"
     gene            1552..1848
                     /locus_tag="VGM72_13680"
     CDS             1552..1848
                     /locus_tag="VGM72_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266364.1"
                     /translation="MGASARGPWYNAKGALIASSVADLHGDAQRDRNSIQKINALDEH
                     GAMIKGFGDMPNEHDMLTGSDSDGRAFSNAFDTTCNNWTSDGADHKAMLGHADR"
     gene            2086..2162
                     /locus_tag="VGM72_13685"
     tRNA            2086..2162
                     /locus_tag="VGM72_13685"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2120..2122,aa:Arg,seq:ccg)
     gene            complement(2217..3683)
                     /locus_tag="VGM72_13690"
     CDS             complement(2217..3683)
                     /locus_tag="VGM72_13690"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY4266365.1"
                     /translation="MDLDRRFLLAAMASAAASPALAQTSPQTVKPQAGEVPLARRLAD
                     YADSVRITDLDGSTIERAKVHLLDSLGCGLAAFKEGTVGTIRELAFANDGGAATILGT
                     KRRATLEWAAFANGAAVRADDINDVYVGRGTGHPSDNVAACLAAAEAAGSSGAEFLLA
                     IVLAYEIEGRFLDAVYLDSRGWDHPNYALISGALAAGRLMKLPPERLTQAVNLALSGH
                     LAMNQTRLGTLSNWKGLAGPEAARSAVFAAQLARAGITGPSPIFEGEAGFFKQVSGRL
                     DIDTAAFGSHGKGFLIHDCFIKSYPAQAQTQTAIPAAAKVAQAVGDLSRIRAIDVKST
                     HLGWLMAGSMPERWAPTTSETADHSLPYIVARAMLDGTITTASYARAALSDPKAAALR
                     KLITVREDAAMTALVPKQSPNSVTATLDDGRVIGERVDDLPGFAGRPMQRADAEAKFT
                     RNAKTLLSPAQIGRIADTVWALERADSVAALIDSLVLT"
     gene            complement(3778..4290)
                     /locus_tag="VGM72_13695"
     CDS             complement(3778..4290)
                     /locus_tag="VGM72_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266366.1"
                     /translation="MRTLVITKASSSQSILSRVVAGKATAAQTESALQQLQALNPHLD
                     INNMKAGTVILIPDAPNFNATEGDPVGSGTFDDFQQLVRSGLSDASQRLRAGDAARAA
                     DRSDINALLKSAAFKKVLDADAALKQQLTDATKTYKDEQTQEDQDEAAVAAAAKLIIA
                     ELADLAKTLG"
     gene            complement(4312..7545)
                     /locus_tag="VGM72_13700"
     CDS             complement(4312..7545)
                     /locus_tag="VGM72_13700"
                     /inference="COORDINATES: protein motif:HMM:NF039478.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6519 domain-containing protein"
                     /protein_id="HEY4266367.1"
                     /translation="MGADFSRVRANPLLDYAGVELQQGRVLLDADFNELNAILDRRLR
                     ALASDTLGRATVSSTTPDAFKISLAGTALQIGKGRLYVDGLLAENHGAVSADPAKRMF
                     DDLLAELQYTDKIDYTAQPYLPNPPVLPTAGRHLVYLDVWDREVTSLEQPSLVETAVG
                     VDTTSRLQTVWQVRVLANDVGSTVTCATPDADVPGWGGIIAPSAGRLTTGTFDVAVVD
                     DPCELPPTGGYRGLENQLYRVEIHDPGQPGGSATIKWSRENLSVGSDVASMISATELE
                     LQTLGRDDVLRFNTGDWVEITDDVREFAQAPGEIRRITVDDATRRIQFTPALPASMLP
                     ASFPDSNFPISRHTRVRRWDQQGLVFRTDTSGTPVQVQDLDASGSTGVIAMPAANIAL
                     LLENGVTVQFSSAGAAGFQAGDYWVFAARTADASVEILDAAPPRGIHHHFARLGIWDV
                     AAGAVTDCRHPWPPQVEGHDCGCTACVTAESHQSGQFTIQDAVNQLSATGGTVCLGPG
                     QYALQDAVRLLNSRSLRIRGQGPATTLVSPAGVFNLQSCIAVAIENLAIVSLGQQAAI
                     TVRTAIGLSLRQLVIAVFGNTDFHGSAIALQGIVAGAEIRDNAIFAQFGIMANDPVAA
                     PPMTGDPAVASFLLTAALAIESNVLWCDRQAITFAGTTLHVMSTHISKNEIAGCRERA
                     VAALGLGAAGSSTVIGRNSFNITGSGIAASVDGLWIEANKLTCNPAAAARAATVGIAL
                     ATGLDKSGPDQCQILGNQLSGFGTGIAIGAPASDLIVKLNILDDCGNGIVSTDNANAG
                     SVSIENNHLRNIGPAAGDASTAMITAIGVARAQTATIAGNTIRIVGGASQQSSLRAGI
                     LAVGVARVRVLGNEITDIAPQGDFLGSAAAIMLRAPYSEFEVTNNQVQRDSGAAIQQA
                     TNGEWFALAAADIDPQNLTSRAGTVTTVSVDSTHVLVLGAGRAYVAALVSTLTAAGAQ
                     GVEPARGSVIANVFNARGNTPAVQISAAGECLFNDNRVDAHLNLKAAVMLTTSTAIVN
                     ANRVRGGEVSIQITGTKSAAVLGNITTGIIGIPGALPAPWDALNLRA"
     gene            complement(7570..9690)
                     /locus_tag="VGM72_13705"
     CDS             complement(7570..9690)
                     /locus_tag="VGM72_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266368.1"
                     /translation="MTSLTADRLYALLPAVYRLRDAASGEPLRALIGALAKEFSALEE
                     NLDQLYDDQFIETCAQWVAPYIGDLIGYRPLHGVSPKIASPRAEVANTIAYRRRKGTA
                     LMLEQLASDVTSWPAHAVEFFQELATSQYMKHIRPQAYATADLRDMGANFLQGGPFNA
                     FAHTTEMRRPEGGSGRYNIPNIGIFLWRLLSLPLSAVPLTPDAADTTGRKFRVNPLGA
                     DLSLFRHAQTEDDITHLAEPINVPEPLSVRLMALAVRASQASTVPAPDAILDDDYGDG
                     ESLVFMRPGTPPSLVPIGQVRVCDLRDILDAGGNVIGWNHEDSVPAGTIGFDPERGRV
                     LLGVPSDGPLLATFYYGVTSAIGGGEYERAPQGDEQANQRTIAGGNPLQPELDAISGG
                     GRLLIGDSLTYAQTPVFKVDDVLAAGAPGLEVVVAAMDSTRPLIAAGGEVTLAVGARG
                     QLVLDGLVFSGGALRLPAAADDETRELILRDCTLVPGLALNPDGSAVSPDVPSLIVEH
                     PFARVTLERCITGPLVIAGDATLTMTDCIVDAGRPESVAYDGDNAGGPGGTLTIRQCT
                     IVGKLHTKLLQLASNSIFFARLADSIGETWAAPIIAERRQEGCVRFCYVPSGSITPRR
                     FRCVPDKDHPSVQPHFTSLRFGDPAYGQLRHATDPAIRQGADDESEMGVLHSLFQPQR
                     ETNLRIRLDEYLRFGLHAGVFYAT"
     gene            complement(9687..12332)
                     /locus_tag="VGM72_13710"
     CDS             complement(9687..12332)
                     /locus_tag="VGM72_13710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEY4266369.1"
                     /translation="MSTPVPDLKPHIPPPLNDCCDCCDGIQAETPEAVENRGGLSSIA
                     YRIGEYAQFRASLHARLSSSDFSALADLRTRDDDDFTIGLIDAFACSADVLTFYQERI
                     ANESYLRTAKERVSLQEMGKLVGYRLRPGVAAETWLAFALETPPVAPPSLAPEPGNFV
                     TGVPSSLGLDIGFKVQSVPGPGEKPQTFETVEALAEARPAWNAMRSWMSEIRRPGRDD
                     TVTYFDSVRNNLKSGDALVFLGDEFLNNQNTNNWDFRVIDTVELQPDNNRTKVTWKRG
                     LGSIEPFKNPAQNPQAFVLRKRAAIFGHNAPIWRAMPGSYRIDYVAHFGGTSDQGDWP
                     AYVLSPLGATTTGGHVDLDTVYSDVQNDGYVVLAKAGFNYPNEPAPSGTYVELYKVTN
                     VAEVSRAEFGISGKLTRVQMAGENYAKFQTTTGTPVRDTSVFAASEGLTFTQYPVDDA
                     VLERVPVATPADGLLPGRKLLVRGNRVSDGAAVIVQATLVAAHAIDATRCELEIAPPL
                     SDALTRDSVVVYANVALASHGESVSQILGAGNASQSFQRFELKQLPLTYRAAANEIGA
                     AAELTVRVGDIAWTERSTMYGAGATERALVLAIDEQGREFVVFGDGVAGARLPSGVNN
                     IRAAYRKGLGVDGNVAAGALTQLMTRPLGLKSASNPLTAQGGTDPESPDAARDSIPIT
                     TRTLGRAVSLLDYEDFARAFSGIAKAQAGVLQLPAGPVVAITIAGPDGTPPLTTASPT
                     WTNLLAALKASGDPHVTIVLLALQASTFRIGLKVKCDPAYDSKSVLAAVEAALRAHYA
                     FDERALGQPVQQSDCIAVAQNVPGVVAVDLTRLYGGTQPLPQTNPSKQIRLLASRMRV
                     ASGLALPAELLTLDSGPLDQLELMT"
     gene            complement(12329..>14529)
                     /locus_tag="VGM72_13715"
     CDS             complement(12329..>14529)
                     /locus_tag="VGM72_13715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HEY4266370.1"
                     /translation="IVANSGSDNPPAGFDPKLSSIEFSFKVECPSDFDCAQNPACPPD
                     SIVRPEIDYLAKDYQGFRRLMLDRLSLLTPGWTERSPADLGVALVELLAYAADNLSYR
                     QDAIANEAYLATARQRVSVRRHARLVDYFLHEGCNARAFVHFDVVGQNVALPSGTQLL
                     TRIANLGTVIAPDSQALRDAINGGAQIFETQYAAILDERLNQIFFYSWGDGGCCLPRG
                     TTSATLAGHVVSLKPGDILVFQEMISPTTFKPEDADRSHRWAVRLTAVTLSSDPSGQL
                     FNEPPVDGPVDVTEITWDAADALPFPLCLSVTARPELKISMAFGNIVLADHGRTIAGE
                     TLGQVPPHTLEISNAAPGACCDTPAPIVVPPRFRPALSQEPLTHGFDIADLLAVPISA
                     DDAWWPASSLLTIDPRSATPRIASLKGTLGPVTEFWTPRRDLMASAEDATDFVVEIEN
                     SGIALIRFGDDDRGKRPDPGTSFLTTYRIGNGVAGNVGADAIAHIVSMANGTFTDLTN
                     PMPAAGGVAPEDMEAARRDAPQAFRTQERAVTPADYAAAAERLPEVQDAAATFRWTGS
                     WYTVFVTPDRLGGADIDAIFAGRLRRHLERFRMAGYDLEIAPPRYVPLNVALHICVLP
                     DYFRADVLAAVAQVLSSQALPDGSLGLFHPDNFSFGQDAYLSRVIAAVQAVEGVDSVR
                     VDIFERLGVPNSTALEEGVIPIGALEIAQLANDPNFPEDGRLTLQAGGGK"
BASE COUNT         2698 a         4538 c         4696 g         2597 t
ORIGIN      
        1 aaggcgatgc tcggccacgc cgaccgcacc ggcggcggca acacgtcgtg gaattcgacg
       61 catatgagcg ccgattgcac caaggccggt ctgatccgga ccggcggcgc aggccatctt
      121 tactgcttcg cgatcaactg attttttcgc gtcttcgttt gcgaagaagg ctcggggcat
      181 ccccgggcct tcttcgtttt ggcggacccg cacgaaattt gccagccaca tttgccagcc
      241 acatttgccc gccgccgcga ggcgtaggcg ggcgcaggat cccatgctat ggtggcggca
      301 tgttcgcgta gggggcggcc ggaccgtcgg gccgataaga agccacggcg ggctgtcagg
      361 tatttcagac cacattcatc cactgggagg cacgatcatg aacaaaacaa ttcttctgtc
      421 cgccgttgca atgacattgg ccggcgcggg cgccgtattg gcggcccagg ctccagcaca
      481 gcccatgagc ttctttatca ccagcacgac gccgaccggt tcgggaaatc tcggcgggct
      541 cgcgggcgcc gacaagattt gccaggacct cgccgcggcg gcaggcgccg gcggcaagac
      601 atggcatgct tatctcagca cacagcccgc caacggcatg ccgggcgtga atgcccgcgg
      661 ccgcatcggc gcgggcccct ggtacaatgc caagggcgtg atgatcgccg ccaacaatgg
      721 cgatctgcat ggcgacaatc agcgcgaccg caacaacatc cagaaagcca acatgctgac
      781 cgagaaaggt gagctgatca aaggcttcgg cgacatgccc aatgagcacg atatcctgac
      841 gggttcggac tccgacgggc gcgcattccc cgccggtctc gacaccacct gcaacaattg
      901 gacctccgac ggcgccgacc acaaggcgat gctcggtcat gccgaccgca gcggcggcgg
      961 caatgtgtcg tggaattcgg tgcatatgag cgccgattgc accaaggccg gtctgatccg
     1021 gaccggcggc gcaggccatc tctactgctt tgcgatcaac tgattttgtc acgttttcag
     1081 ttacaggaag gctcggggtg catccccggg ccttcttcgt tttcatcccg cctttcagcg
     1141 cgacgtcatt tcagcgcacc cgtgtggcgc gctttcatcg ccatgttatg gtgaatcgca
     1201 tgaaaccggc ttgggcaatg ccgaggcatt gcctgggaag aaacaccgca acgagactta
     1261 gcattcacgg ggagggcgca agcatgaccg ccaaatggac ctttctttcc gccgcaaccg
     1321 tcacactcat cggttctgcc gccgtgctgg cgcaaggcca accggcagca ccgcagcacg
     1381 cgatgacgtt cttcatcgcc ggcgccgttc caggcaccgg caatctcggg gggctcaaag
     1441 gcgcggacaa gatttgccag aatctggcaa cagcggtggg cgcgggcgcg cacacctggc
     1501 acgcctatct cagccagcag gcgcaaggca ccctgccggc ggtgaatacg cgtgggcgca
     1561 tcggcccggg gcccctggta caatgccaag ggcgcgctga tcgcctccag cgtcgccgat
     1621 ctgcacggcg acgctcagcg cgaccgaaac agcattcaga agatcaacgc gctggatgag
     1681 cacggggcca tgatcaaggg tttcggcgac atgcccaatg agcatgacat gctgacggga
     1741 tcggattccg atgggcgggc attctcgaac gccttcgaca ccacctgcaa caattggacc
     1801 tccgacggcg ccgatcacaa ggcgatgctc ggccatgccg atcgctaggg cgggggcacc
     1861 acctcgtgga attcggccca tatgagccgc gattgcacaa aggcggggct tatcgcgacc
     1921 ggcggggcgg ggcacttcta ttgcttcgcg gtcaactgac gcgccaccgc cgtgcaccgg
     1981 ccacattgca aaaggtccgg gatgttccga ccttttgttc gccggtgcta ttcttgacct
     2041 ttgcgcgaaa gccccggcct gggctagggt cgccggccat tgctcgcacc cgtagctcag
     2101 ctggatagag tgctgccctc cgaaggcaga ggtcacaggt tcgaatcctg tcgggtgcgc
     2161 caataatttc agtcacttat aattttccga ggcactctca caggagcgca ggccgcttac
     2221 gtcaagacca gactgtcgat cagcgcggcc accgaatcgg cacgctccaa cgcccagacg
     2281 gtatcggcga tgcgcccgat ctgcgcgggg ctcagaagcg ttttggcgtt gcgcgtgaat
     2341 ttcgcttccg catcggcgcg ctgcatggga cggcccgcga atcccggcag atcgtcaacc
     2401 cgttcgccga tcacacggcc atcgtcgagt gtcgccgtga cgctgttcgg actttgcttc
     2461 gggaccagcg cggtcatggc cgcgtcttcg cgcacggtga tcagctttct gagggccgcg
     2521 gctttgggat cgctcagagc agcgcgcgcg taactcgccg tcgtgatcgt gccatcgagc
     2581 atcgcgcgcg ccacgatata gggcaggctg tggtctgcgg tctccgatgt cgtcggcgcc
     2641 cagcgttcgg gcatgctgcc ggccattagc cagccgagat gcgtcgattt cacgtcgatg
     2701 gcgcggatgc gcgacagatc gccgacggcc tgggcaacct tcgcggcggc cggaatcgca
     2761 gtttgagtct gcgcctgcgc cggataggat ttgatgaagc aatcatggat cagaaaacct
     2821 ttgccgtggc tcccaaaggc ggcggtatcg atgtcgagcc tgcctgacac ctgtttgaag
     2881 aatcccgctt cgccttcgaa gatcggcgat ggcccggtga tgccggcgcg cgccagttga
     2941 gccgcgaaga cggcgctacg cgccgcctcg gggccggcca accctttcca attcgagagc
     3001 gtgccgagcc gggtctggtt catcgcgaga tggccgctca gcgcgagatt cacggcttgc
     3061 gtgagacgct ccggtggcag tttcatcaat ctgcccgcgg cgagcgcgcc ggaaatcagg
     3121 gcatagttgg gatgatccca gccgcgcgag tcgagataca ccgcatcgag aaagcggccc
     3181 tcgatctcat aggccagcac aatggcgaga aggaactcgg cgccggagga acccgccgct
     3241 tcggccgcgg caaggcaggc cgcgacattg tcactggggt ggccggtgcc gcgtccgaca
     3301 tagacatcgt tgatgtcgtc ggcgcgaacg gccgcgccat tcgcgaacgc cgcccattcg
     3361 agcgtcgcac gccgcttggt cccgagaatc gttgccgcgc cgccgtcatt ggcgaaagca
     3421 agctcgcgta tggtgccgac cgtcccttcc ttgaaggccg ccagcccgca accgagcgag
     3481 tcgagcagat gcacttttgc ccgctcgatc gtggacccat cgagatcggt aatgcgaacg
     3541 gaatcggcat aatcggcaag gcggcgggcc aacggaactt caccggcctg gggcttcacc
     3601 gtctgaggcg aagtctgggc caaggccgga gacgccgcgg cgctggccat tgcggcaagg
     3661 agaaagcggc gatcgagatc catggctata ttcctcagcc cgagagcgcg acgctagcat
     3721 ttggctccat tgcacgaaag cttgggaccc tgtttgtaaa accgtgtttc tctttgttca
     3781 gccaagcgtt tttgcgaggt ctgccagttc ggcgatgatt aacttggccg ccgcggcgac
     3841 cgccgcttcg tcttgatctt cctgcgtctg ttcgtccttg taggtcttgg tggcgtcggt
     3901 gagctgctgc ttgagtgccg catcggcatc caggactttc ttgaacgccg ccgacttcag
     3961 gagcgcgttg atgtccgagc ggtccgcggc gcgcgcggca tcgcccgccc gcagcctttg
     4021 ggacgcatcg gaaagaccgc tgcggacgag ctgctggaaa tcgtcgaatg ttccgctccc
     4081 caccggatcg ccttcggttg cgttgaagtt cggcgcgtcg ggaatgagga tcacggtccc
     4141 cgccttcata ttgttgatgt ccagatgcgg gttcagcgcc tggagctgct gaagcgcgga
     4201 ttcggtctgg gcggcggtgg ctttgcctgc gaccacgcgg ctgaggatcg actggctgct
     4261 ggaggctttg gtgatgacga gcgtgcgcat gatgttgcct ttttcttgag attaagcgcg
     4321 cagattgaga gcgtcccacg gggccggcaa tgcgccggga atccctatga tgccggtcgt
     4381 gatattgccg agcaccgctg cggatttcgt acccgtgatc tggatcgaga cttcgccgcc
     4441 acggacgcga ttggcgttca cgatggcggt gctcgtcgtc aacatcacgg cggcctttaa
     4501 attgagatgc gcatccacgc gattgtcgtt gaacaggcat tctcccgcgg cggagatttg
     4561 gaccgccggc gtattgccgc gcgcattgaa aacatttgcg atcactgaac cgcgcgccgg
     4621 ctcgacaccc tgcgcacccg cggccgtcaa agtgctgacg agagccgcga cataggcacg
     4681 gcccgcgccc agcaccagga cgtgggtgct gtcaacgctg accgtcgtca cggtgcctgc
     4741 ccgcgatgtg aggttttgcg gatcgatatc ggcggcggcg agtgcgaacc actccccgtt
     4801 cgtggcctgt tgaatggctg caccggaatc gcgctgcacc tgattgttgg tgacctcgaa
     4861 ctcggaatag ggcgcacgca acatgatggc cgcagcggag ccgaggaagt ccccctgcgg
     4921 cgcgatgtcg gtgatttcgt ttccaagcac gcgcacgcgc gccacgccaa cagccaggat
     4981 ccccgcacgc aaggaggatt gttgtgacgc gccgcccacg atgcggatcg tattgccggc
     5041 aatggtggcg gtctgagccc gcgccacgcc aatggccgtg atcatcgcgg tgctggcgtc
     5101 ccccgcggcg ggaccgatgt tgcgcaaatg attgttctcg atggatacgg atcccgcatt
     5161 ggcgttgtcc gttgaaacaa tgccattgcc gcaatcatca aggatattga gcttgacgat
     5221 cagatcgctc gcgggcgcac cgatcgcgat gcctgtgcca aatccagaca attgattgcc
     5281 gagaatctgg cactgatccg gtccgctctt atccaatcct gtcgctagcg cgatccccac
     5341 cgtcgcggcg cgtgccgcgg cggccgggtt acaggtgagt ttgttggctt cgatccagag
     5401 cccgtcgacg ctggccgcga tgccgctacc ggtgatgttg aagctgttac gaccgatgac
     5461 cgtggaagaa ccggccgccc ccagcccgag cgcggcgacc gcacgttcgc ggcaaccggc
     5521 aatttcgttt ttgctaatat gcgtgctcat cacatgcagg gtggttccgg caaatgtgat
     5581 agcctggcga tcacaccaga gaacattgct ttctatggcg agcgcggccg tcagcaggaa
     5641 gcttgccacc gcggggtctc cggtcatcgg tggcgccgcg acgggatcgt tcgccatgat
     5701 cccgaattgc gcgaagatcg cgttgtcgcg gatttcggcg cccgcgacga ttccttgcag
     5761 cgcaatcgcg gagccatgga agtcggtgtt cccaaaaacc gcgatcacca attgacgcag
     5821 agaaagcccg atggccgtgc gcaccgtgat ggccgcttgc tgccccaagg acacgatcgc
     5881 cagattttcg atcgccaccg cgatgcagct ctgcaggttg aagacgcctg ccggactcac
     5941 caaggtcgtg gccggtccct gtccgcgaat gcgcaacgag cgcgaattga ggaggcgcac
     6001 ggcgtcctgc agcgcgtatt gaccggggcc gagacagacc gttccgccgg tcgcgctcaa
     6061 ttggttgacc gcatcctgga tagtgaactg accgctttga tgggattcgg cggtcacaca
     6121 ggcggtgcag ccacaatcat gtccttcgac ctgcggcggc caggggtggc ggcaatccgt
     6181 caccgcgccc gcggccacat cccagatgcc gagccgggca aaatgatgat ggatgccacg
     6241 cggcggcgcg gcgtccagaa tctcgacact ggcatctgcg gtgcgcgcgg cgaacaccca
     6301 ataatcgccc gcttggaacc cggccgctcc ggcggacgag aattggacgg tgacgccgtt
     6361 ttcgagcagc agggcgatgt tcgcggccgg catggcgatg acgccggtgg aaccggacgc
     6421 atcgagatcc tgcacctgaa cgggtgtgcc gctggtatcg gtccggaaca cgagcccctg
     6481 ctgatcccaa cggcgcacgc gtgtatggcg cgaaatcgga aaattgctgt cgggaaagct
     6541 tgccggcagc atgctcgccg gcagcgcggg cgtgaattgg attcgccgtg tcgcatcgtc
     6601 cacggtgata cggcggatct cgccgggcgc ttgggcgaat tcgcgcacat cgtcggtgat
     6661 ctccacccaa tcgccggtgt tgaaccgcag cacgtcgtcg cgtccgagcg tttgcagttc
     6721 cagctcggtc gccgaaatca tgctggcgac atcgcttccc acgctcagat tctcgcggga
     6781 ccatttgatg gtggcactcc cgccgggctg gcccggatcg tggatttcca cgcggtagag
     6841 ctggttctcc agcccgcgat aaccacctgt cggcggcagc tcgcaggggt cgtccaccac
     6901 cgcgacatcg aatgtgccgg tggtcagcct gcccgcggac ggcgcaatga tcccacccca
     6961 gcccggaaca tcggcatcgg gcgtcgcaca ggtgacggtg cttcccacat cattggcaag
     7021 cacgcgcacc tgccagacgg tctggaggcg tgacgtcgta tccacaccga cggcggtctc
     7081 cacgagcgag ggctgttcca ggctcgtgac ttcgcgatcc cacacatcga gatagacgag
     7141 atggcgcccc gcggtcggca aaaccggagg gttcgggaga tagggctgcg ccgtatagtc
     7201 gattttgtcg gtgtattgca gttccgcgag cagatcgtcg aacatgcgct tcgccggatc
     7261 ggcggaaacc gcgccgtgat tctccgcaag caggccatcg acatacagcc ggcccttgcc
     7321 gatctgaagc gccgttcccg cgagactgat cttgaacgca tccggcgtcg tcgaggagac
     7381 cgtcgcgcgc ccaagcgtat cgctcgccag cgcccgcaac cgccggtcta gaatcgcatt
     7441 cagctcgttg aagtcggcgt cgagaagaac gcgcccctgc tgaagctcca cgcccgcata
     7501 atcgagcaac ggatttgcac gaactcttga aaaatcggcg cccatggcgg tcctccagac
     7561 agattcgatt caggtggcgt agaacacgcc ggcgtgaagc ccgaagcgca gatattcatc
     7621 gagccggatg cgcaaattgg tttcgcgttg cggttggaaa agcgaatgca gaacgcccat
     7681 ctcgctttcg tcgtcagcgc cttggcgaat tgcgggatcg gtggcgtggc ggagttgccc
     7741 ataggccgga tcgccaaagc gcaaggaggt gaaatgcggc tgcaccgaag ggtgatcctt
     7801 gtcgggtacg caacgaaagc ggcgcggggt gatcgagccg gacggcacgt agcagaaacg
     7861 cacacaccct tcctgccgcc gctcggcgat gatgggtgcc gcccatgtct cgccgatgga
     7921 atccgcaagg cgtgcgaaaa atatgctgtt cgacgcaagt tgaagcagct tcgtatgaag
     7981 cttgccgaca atcgtgcact gccggatggt cagcgtgccc cccggaccgc ccgcattgtc
     8041 gccgtcatac gcgacgctct cgggcctgcc cgcatccacg atgcaatcgg tcatggtgag
     8101 tgtcgcatcg ccggcgatca caagcggccc cgtgatgcag cgctccagcg ttacgcgcgc
     8161 gaaggggtgt tcgacgatca ggctcggcac gtccggcgaa accgcgctgc cgtcggggtt
     8221 gagcgcaaga ccgggaacaa gcgtgcagtc gcgcaagatc agctcgcgcg tttcatcgtc
     8281 ggcggccgcg ggcaggcgca aggcgccgcc gctgaacacc agaccatcca gcacaagctg
     8341 cccacgcgcg ccgacggcga gcgtcacctc gccgcccgca gcgatcagcg gacgcgtgga
     8401 atccattgcg gcgacaacaa cctcaagacc gggcgcgccc gcagccagga catcatccac
     8461 cttgaacacc ggcgtctggg cataggtcag gctgtcgccg atcaaaagcc ggcccccgcc
     8521 ggagatggca tcgagttcgg gctgcagcgg attaccgccc gcaatggtgc gttgattggc
     8581 ctgctcatcg ccttgcggcg cgcgctcata ttcgccaccg ccgatggcgc tcgtcacccc
     8641 ataatagaac gtcgcaagca atggcccatc ggacggcaca ccgagcagga cccgcccgcg
     8701 ttccggatcg aagccaatgg ttccggccgg aaccgaatcc tcgtggttcc agccgatgac
     8761 atttccgccc gcatccagaa tatcgcgcag atcgcaaacc cgcacctggc cgatcggcac
     8821 aagcgaaggc ggcgtgccgg gacgcatgaa cacgaggctc tcgccatcgc cataatcatc
     8881 gtccagaatc gcgtcgggcg ccggtacggt gctcgcctgc gaggcgcgca cggcaagcgc
     8941 catcagacgg acggacagag gttcggggac attgatcggc tcagccagat gcgtgatgtc
     9001 atcttcggtt tgggcgtggc ggaagagact cagatcggcg ccgagcggat tgacgcggaa
     9061 tttgcgtccc gtcgtgtccg cggcatcggg cgtcagcgga acggcactta agggaagcga
     9121 aagcagccgc cacaggaaaa tgccgatgtt ggggatgttg tagcgccccg aaccgccttc
     9181 cggccggcgc atttccgtgg tgtgcgcgaa cgcgttgaag ggcccgccct gcagaaaatt
     9241 cgcccccata tcgcgcaaat cggcggtcgc gtaagcctgc ggacggatgt gcttcatata
     9301 ttggctggtg gcgagctctt gaaaaaactc caccgcatgg gccggccagc tcgtcacgtc
     9361 gctcgccaat tgttcgagca tcagggccgt gcccttgcgg cggcgataag cgatggtgtt
     9421 ggcaacctcg gcgcggggcg aagcgatttt cggcgacacg ccatgcagcg ggcgatagcc
     9481 gatcagatcg ccgatatagg gcgccaccca ttgcgcgcag gtctcgatga actgatcgtc
     9541 gtaaagctgg tcgagatttt cttcgagcgc tgagaattcc ttggcaagcg cgccgatgag
     9601 cgcgcgcaac ggttcgccgg acgcggcgtc gcgcagacgg tacaccgccg gcaggagcgc
     9661 gtagagacga tcggccgtga ggctcgtcat gtcatcagct ccaattgatc gagtggtccg
     9721 gaatcgagcg tcaaaagctc agccggcagc gcgagcccgc ttgccacgcg catgcgcgag
     9781 gcgagcaggc ggatttgctt ggacgggttg gtctgcggca agggctgcgt gccgccgtaa
     9841 agccgggtga ggtcgaccgc gacgacacct gggacatttt gcgcaaccgc gatacagtcg
     9901 gattgctgaa ccggctgacc cagggcgcgc tcatcgaacg cgtaatgggc ccgaagcgcc
     9961 gcctcgaccg cagcgagaac gcttttggaa tcataggctg gatcgcattt gaccttcagt
    10021 ccgatgcgga acgtgctcgc ctgaagcgcc agcagcacaa ttgtcacatg cggatcgccg
    10081 ctcgctttga gcgcggcaag aagattggtc caggtcgggc tcgcggttgt cagcggcggc
    10141 gtgccgtcag gcccggcaat ggtgatggcg acgaccggac cggcgggaag ttggagcacc
    10201 ccggcctggg ctttggcgat gccgctgaaa gcgcgcgcga aatcctcata gtccagcaga
    10261 gataccgcac ggccgagcgt gcgtgtggtg atcggaatgc tgtcgcgcgc ggcgtcggga
    10321 ctttccggat cggtgccgcc ttgcgcggtc aggggattgc tggcgctctt caaacccagc
    10381 ggacgcgtca tgagctgcgt cagcgcgccc gccgccacgt tgccgtcgac gcccagcccc
    10441 ttgcggtagg ccgcgcgaat gttgttgacg ccgctcggca ggcgcgcgcc cgcaacgcca
    10501 tcgccgaaca ccacgaattc gcggccctgc tcatcgatcg cgagcacaag tgcgcgttcg
    10561 gttgcgcctg cgccatacat cgtgctgcgt tccgtccagg cgatgtcgcc gacgcgcacc
    10621 gtgagttcgg ccgcagcccc gatctcgttc gcggccgcgc gataggtgag gggcaattgc
    10681 ttcagctcga agcgctgaaa gctctggctc gcattgcccg cgccgagaat ctgcgaaacc
    10741 gattccccgt gtgaggcaag cgcgacattg gcgtacacca cgacgctatc gcgcgtcagg
    10801 gcgtccgaaa gcggcggcgc gatctcaagt tcacagcggg tggcatcgat tgcgtgggcg
    10861 gcgaccaatg tcgcttgcac gatcaccgcg gcgccgtcgc tgacacgatt tccgcgcacg
    10921 agcaatttgc ggcccggcag aagcccatcg gcgggcgtgg ccacgggcac acgttccaaa
    10981 acggcatcat cgaccggata ttgcgtgaat gtcagcccct cgcttgcggc gaagacgctc
    11041 gtatcccgca caggcgttcc ggtggtggtc tggaatttgg cgtaattctc accagccatc
    11101 tgcacccgcg tcagcttgcc ggaaatccca aattcggcgc gcgacacttc agcgacattg
    11161 gtcaccttgt acagttcgac ataggttccc gacggcgcgg gttcgtttgg ataattgaag
    11221 cccgccttgg cgagaacgac atagccatca ttctgcacat cggaatagac ggtatcgaga
    11281 tcgacgtgac ctcctgtcgt ggttgcgcca agtggggaca gcacataggc cggccagtcg
    11341 ccttgatcgg aggtgccgcc gaaatgcgcc acataatcga tgcggtacga acccggcatc
    11401 gcacgccaga tcggcgcgtt gtggccgaag atcgcggccc gcttgcgcag cacgaaggcc
    11461 tgcgggttct gcgctggatt cttgaagggc tcgatcgagc ccaggccgcg tttccaggtc
    11521 accttcgtgc gattgttgtc gggctgcagc tcgaccgtgt cgatcacccg gaagtcccaa
    11581 ttgttggtgt tctggttgtt gaggaactcg tcccccagga acaccagggc gtcgccggat
    11641 ttgagattgt tccgtacact gtcgaaatag gtgacggtgt cgtcgcgccc gggacgacgg
    11701 atctcgctca tccaggagcg catcgcgttc catgcgggcc gtgcctccgc cagcgcctcg
    11761 acggtctcga aggtttgcgg cttttcgcca ggccccggca cgctctgaac cttgaacccg
    11821 atatcgagcc cgagactcga aggcacgcca gtgacgaaat tgccgggctc gggcgcgagc
    11881 gatggcgggg ccaccggcgg ggtttcgagc gcgaaggcga gccaggtttc cgcggccacg
    11941 ccggggcgca aacgatagcc gacgagcttg cccatctcct gcagcgagac gcgctccttg
    12001 gccgtacgca gataggattc gttggcgatg cgctcctgat aaaatgtgag cacatcggcc
    12061 gaacaggcga acgcatcgat caggccgatg gtgaaatcat cgtcatcgcg cgtgcgcaaa
    12121 tccgcgagcg cggagaaatc cgaagacgac aggcgggcgt ggaggctggc gcggaactgc
    12181 gcatattcgc caatgcgata ggcgatggag gacaggccac cgcgattctc caccgcttcg
    12241 ggcgtttccg cctggatgcc gtcgcagcaa tcgcagcaat cgttcaacgg cggcggaatg
    12301 tggggcttca gatccggaac gggcgtgctc atttgccgcc tccagcttgg agcgtcagcc
    12361 gcccgtcctc ggggaaattg ggatcgttgg caagctgcgc gatttcgagt gcgccgatcg
    12421 gaatgacacc ttcttcaagc gcggtggaat tcggaacgcc aagccgctcg aaaatatcga
    12481 cgcgcaccga gtccacccct tcgaccgctt ggaccgctgc gatgacccgg ctgagatagg
    12541 cgtcctgtcc gaacgagaaa ttgtccggat ggaacagacc aagactgccg tcaggcagcg
    12601 cctggctgga aagtacttgg gcaaccgccg ccagcacatc ggcgcggaaa taatcgggaa
    12661 gaacgcagat atgcagcgcg acgttcagcg gcacatagcg cggcggcgcg atctcgagat
    12721 cgtagcctgc catgcggaag cgttcgaggt gacggcgcaa gcgccccgcg aagatcgcgt
    12781 cgatgtcggc gccgcccaac cggtccggcg tcacaaacac ggtgtaccag cttcccgtcc
    12841 agcggaacgt cgccgccgca tcctgcactt cgggcagacg ttcggcggcg gccgcgtaat
    12901 cggccggcgt caccgcccgc tcttgtgtcc ggaaggcttg cggtgcatcg cggcgcgcgg
    12961 cttccatgtc ctcgggcgcg acaccgcccg ccgccggcat cggattggtg aggtccgtaa
    13021 aggtcccgtt cgccatgctc acgatatgag cgatggcatc ggcgccgaca ttacccgcga
    13081 caccgttgcc gatgcgatag gtggtaagga aactggttcc gggatcgggc cgtttgccgc
    13141 gatcgtcgtc cccgaagcgg ataagggcga tgccgctgtt ctcgatctcc acgacgaaat
    13201 cggtggcgtc ctccgcactc gccatgagat cgcggcgcgg cgtccagaat tccgtcaccg
    13261 gaccgagcgt gcctttgagt gacgcgatcc gcggcgtggc gcttctcggg tcgatcgtca
    13321 ggagcgaact ggcgggccac catgcatcgt cggcgctaat cggcaccgcg agcagatcgg
    13381 cgatgtcgaa gccgtgcgtc aggggttcct gtgagagggc agggcggaaa cgtggcggaa
    13441 cgacgatggg cgccggtgtg tcgcaacaag cgccgggcgc tgcgttcgag atttccagcg
    13501 tgtgcggagg cacttggccg agcgtttcgc cggcgatagt gcgcccgtga tcggccagca
    13561 cgatattgcc gaacgccatg ctgatcttga gctccgggcg cgcggtaacc gacagacaca
    13621 gcgggaatgg cagcgcgtcg gcggcatccc aggtgatttc ggtgacatcg acaggaccat
    13681 cgaccggcgg ttcgttgaag agctgccccg aggggtcgct ggaaagcgtg acggcggtga
    13741 gccgcaccgc ccagcgatgc gagcggtcgg catcttcggg tttgaacgtc gtggggctta
    13801 tcatctcctg gaacaccagg atgtcgccgg gtttcaacga aacgacgtgg cccgcgagcg
    13861 tggcgctggt cgtgccgcgc ggcagacagc atccgccgtc accccagcta tagaaaaaga
    13921 tttggttcag gcgctcgtcc aggatcgcgg cgtactgggt ttcgaagatc tgggcgccgc
    13981 cattgatggc atcgcgcaac gcctgactgt ccggcgcgat caccgtaccg agattggcaa
    14041 ttcgggtcag caattgcgtg ccggagggca gggccacatt ctgcccaacc acatcgaaat
    14101 gcacgaaggc gcgcgcattg cagccttcgt ggaggaaata atccaccaat cgcgcatgac
    14161 ggcggacaga aacgcgctga cgcgccgtcg caagataggc ttcgttggcg atcgcatcct
    14221 ggcggtaaga gagattgtcg gccgcgtagg cgagcaactc gaccagggcc acgcccagat
    14281 cggcggggga gcgttccgtc cagcccggtg tcagcagact caaccggtcg agcatcaacc
    14341 gccggaagcc ctgatagtcc ttggcgagat agtcgatctc gggacggacg atcgagtcgg
    14401 gcggacaggc gggattttgc gcgcagtcga aatccgacgg acattccact ttgaaggaga
    14461 attcgatgga cgacagtttg gggtcgaaac ccgccggtgg attgtcgctg ccggaattgg
    14521 cgacaatgg
//
LOCUS       DASYEG010000326        10247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4480604, whole genome shotgun sequence.
ACCESSION   DASYEG010000326 DASYEG010000000
VERSION     DASYEG010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 10247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10247
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4480604"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VGM72_13720"
     CDS             complement(<1..480)
                     /locus_tag="VGM72_13720"
                     /inference="COORDINATES: protein motif:HMM:NF014873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266371.1"
                     /translation="MQALIEAIDGLPRLTRFGRVARVEGLLVEVTGASGAIGLGGRLR
                     ISGADNRETPCEVVGFRDGRALAMPFGQLEGITLGARATFEENVSAIYPSRAWLGRVI
                     DAFGNATDGKGPLVCGMEAYPLRAAPPSAHARARLGGKLDLGVRALNAFTTCCKGQRM
                     "
     gene            743..1435
                     /locus_tag="VGM72_13725"
     CDS             743..1435
                     /locus_tag="VGM72_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4266372.1"
                     /translation="MRVLLIEDDSAMARSIELMLRSEGFNVYTTDLGEEGIDLGKLYD
                     YDIITLDLSLPDMSGFEVLKSLRLAKVQTPILILSGNAIVEAKVKALGFGADDYMTKP
                     FHKDELVARIQAVVRRSKGHSQSVITTGKLTVNLDAKTVEVDGSRVHLTGKEYQMLEL
                     LSLRKGTTLTKEMFLNHLYGGMDEPELKIIDVFICKLRKKLAVACNGEHYIETVWGRG
                     YVLRDPDEKIAA"
     gene            complement(1496..2341)
                     /locus_tag="VGM72_13730"
     CDS             complement(1496..2341)
                     /locus_tag="VGM72_13730"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEY4266373.1"
                     /translation="MPLRQEDFRFLAELMKRTSGLVVTQDRANLILSRLQPVAELHGF
                     ADVSHLVADLRYGNEPLTRAVIEAMTIRDTSFFRDSAAFKSFEDTLLPRLLRSRMAKR
                     RIAIWSAACATGQEPYSIAMIFARLPQFAGWDIDILATDVSADAVARAKTGLYTHGEV
                     QRGLPLQMLANHFRPQAKEWRIRSGIRSRVVFRVLNLLDPFVSLGPFDVIFCRNVLMY
                     FDSATKTGILDRLSDTLADDGYLVLGSAETTLGSGSSFASERPGRAITMKAARAQMSR
                     AAAFG"
     gene            complement(2346..2711)
                     /locus_tag="VGM72_13735"
     CDS             complement(2346..2711)
                     /locus_tag="VGM72_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4266374.1"
                     /translation="MKQCLIVDDSRVLRKIARRILQELQYEVEEAEDGAAALDVCRRG
                     MPDAILLDWNMPAMSGMEFLRALRREPEGGKPIVVYCTTENDIAHITDAIGAGANEYL
                     VKPFDKDIVEAKFAEAGLM"
     gene            complement(2828..3469)
                     /locus_tag="VGM72_13740"
     CDS             complement(2828..3469)
                     /locus_tag="VGM72_13740"
                     /inference="COORDINATES: protein motif:HMM:NF021595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphotransferase family protein"
                     /protein_id="HEY4266375.1"
                     /translation="MLNTKGLAMNDLDFAALLVSRVCHDLVSPVGAVVNGLEVLEDET
                     DLAMRADALRLVAASAEQAASRLKFCRIAFGAAGSAGAELDLSEVGRIMNGLLKGGKI
                     DLLWQAEPTNWPKDWAKLLMNAVLATGDCLPRGGRIIVETSGTPEAPAFRVIGTGTVV
                     RLMSEVERALSGEPEGLLDGRSIQPYLTYQLAKTLDARLTIACLPNEVSFTAG"
     gene            3557..4354
                     /gene="cysQ"
                     /locus_tag="VGM72_13745"
     CDS             3557..4354
                     /gene="cysQ"
                     /locus_tag="VGM72_13745"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEY4266376.1"
                     /translation="MTVFISEFTARLAEIAHDAGQVILKHYAAGVPIRFKQDASPVTA
                     ADEEAEALILERLADVAPGVPIVAEEEMAAGRRPRRLGTRFFLVDPLDGTKEFLKRNG
                     EFTVNIALVEQGRPILGVVLAPAKARAFIGDGVNGAFELAAPEDAQLDMGQARRIAAR
                     KAPEGGLTVIASRTHGEPATEEYLRSHRIAHRMTAGSSLKFCLVAAGEADLYPRLGRT
                     MEWDTAAGQAVLEAAGGSVNTLDGAPLRYGKIAQAFANPHFVARGIR"
     gene            4433..5785
                     /locus_tag="VGM72_13750"
     CDS             4433..5785
                     /locus_tag="VGM72_13750"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY4266377.1"
                     /translation="MTGLEVKNWFGDLISHPKIIVDANSVQDIVAILKDKAKYPSPVR
                     AVGSNHSTAPCGVAEGGTLIRMKMNRILSIGADSITVEAGARHIDMAKELQKHNLQFY
                     VNTEIGNLTAGSAACAGTKDASFPGEYGQVGSYVIGIKLVLPNGDLLEVTEADPGLMQ
                     QVRSSYGTFGIVYEVTYRIRPLLPMAVYHETFTLEEFVTKLDALKARGQSMMFYYFPF
                     ADRITIEFRSYNPNATGSPNRSAWTLRNYLWGTAGPRFGHDIEATIPVPAVRYEVIDG
                     FNAILRFKLEHLIRADYTLAPDQIINYPDVSDNSRYTFSLFAFPETQFPQTLSEFFKF
                     ARDYYDKQGYRSNLLYVGYHIAQDQQSLLSYSYDGPVMTIDPVSTGNNGWKPFLETYN
                     QFCIARGGKPLFNQTYGLTADIVQKAFGERLRTFAGTRRKFDPDGRLLNDYFRMLLSA
                     "
     gene            complement(5798..6979)
                     /locus_tag="VGM72_13755"
     CDS             complement(5798..6979)
                     /locus_tag="VGM72_13755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY4266378.1"
                     /translation="MRRAYWTRRRLAAAIVFTVLLLGMILWWPFGSSQPTIVYGVAKA
                     ARTTVEATVHSTGTLEPRNPVDIVAPASGRLQSLAVKNGDRVSKGQVLARLVSETARA
                     DLLSVQAELAARQADLARSQIDIAEARAALMRARNDMRPGTADAAEARLARVSANGDE
                     ARKLVQAAQQSVASSRNVLASLDIRAPFDGTVLKIGYDPSSDVRAVSRGQPLLTMVAD
                     LSELNVTTAFPENALGALRAGERAGFTVPAYPQRTFSAALTALDLWPERQIRDGRQVV
                     TYRGTLAAANPGDMLRPGMSASIAIIAAESKNVLTVPNGALAFAPPPKIESRFAPMKQ
                     PAGAAHFGRVWVLANGKPEPRDIALGLSDGRVTQVVSGSLREGDNIITSAVITKRGGQ
                     S"
     gene            complement(7374..7625)
                     /locus_tag="VGM72_13760"
     CDS             complement(7374..7625)
                     /locus_tag="VGM72_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266379.1"
                     /translation="MANNSVHGPKRSPLGAGGWIAILAMGILLGAAIWFLFYGWHLTD
                     APIDTNGYIALGLGVVLSMVLGGGLMGLLFWSNRKGYDR"
     gene            7852..8442
                     /locus_tag="VGM72_13765"
     CDS             7852..8442
                     /locus_tag="VGM72_13765"
                     /inference="COORDINATES: protein motif:HMM:NF018302.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1134 domain-containing protein"
                     /protein_id="HEY4266380.1"
                     /translation="MSIFKILRAGLGAIVLSFALAATASAAPARDPRDPDTYSPEEVV
                     KAGADFFGVTTEAMAKAVEKVLSRYGRPNAYIAGSEGSGAVVVGLRYGEGELYMKGKG
                     APEKVFWQGPSIGFDLGANASKTFTLVYNLPSPESIFERFPGVEGSAYFIAGIGVNYQ
                     QNGKIILAPMRTGVGLRYAANIGYLSYSKERNWVPF"
     gene            complement(8455..9111)
                     /locus_tag="VGM72_13770"
     CDS             complement(8455..9111)
                     /locus_tag="VGM72_13770"
                     /inference="COORDINATES: protein motif:HMM:NF017833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEY4266381.1"
                     /translation="MTQQVDAVAAEGSPRPIRTSELLREFANSLHSDRVTLAEIVSGL
                     GDRGLGVLIAIFALPNILPSTVPFGNVATGIPPLIFAVQLLLGVEHLILPGFMGRWKI
                     GTHWLKALAPRVAAVLSWFERLLTPRMEWVTGQRAERFIGIIAIILAMVSTLPIPFGH
                     NLPALGLVLIGLGLIERDGLAILIGSGIGLLGSILLGLVIFGVAHGLSFIIRAHHVHF
                     "
     gene            complement(9114..>10247)
                     /gene="secF"
                     /locus_tag="VGM72_13775"
     CDS             complement(9114..>10247)
                     /gene="secF"
                     /locus_tag="VGM72_13775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY4266382.1"
                     /translation="ITFASSLPAIAFLFRVPKARAVTLGDMPDPFPLAWPGFAAMSHP
                     PQGHFRRIFQRMRLLKLIPDHTNIDFVGARFYAFAIDGLLVLAALISLLVHGLNFGID
                     FTGGVLMEVKAAQSIDIGAMRTKVDNLHFAEAQLQYFGGGECEMPVNSCILIRVQPEV
                     NAPASDQAVVNTTRTALGEGYAFRRTEVVGPKVSDELRNAGILATVIAVFMIGLYVAV
                     RFEWQYGVSAIIATGHDVFVTAGLFSLMHLDFTLTSVAALLTLAGYSVNDTVVVFDRI
                     RENRRRYKRIGLKELINISTNQTLSRTVLTSGATAISIIPLLLFGGPTLFDFTAAILF
                     GIVVGTFSSIYVASALLIYLPAVTGGSDGPTAAAPVTASARAR"
BASE COUNT         2112 a         3122 c         3120 g         1893 t
ORIGIN      
        1 catgcgctgg cctttgcagc aggtcgtaaa ggcattgagc gctcgcacac ccagatcgag
       61 tttgccgccg agacgcgcgc gtgcatgggc cgaaggcggc gcggcacgca gcggataagc
      121 ctccatcccg caaaccagcg gtcccttgcc atcggtcgca ttgccgaacg cgtcgatgac
      181 gcgccccagc caggcccggc tgggatagat tgccgagacg ttctcctcga aggtcgcgcg
      241 tgctccgagc gtgatgccct caagttgacc gaacggcatc gcgagcgcgc gtccgtcgcg
      301 gaaacccaca acttcgcaag gggtttcgcg attgtccgcg ccagaaatcc gcagacgtcc
      361 gcccaggcca atagcgccgg aggcgcctgt cacttcgacc agcagcccct caacccgggc
      421 gacacgtccg aaacgggtga ggcgcggcaa accatcgatg gcttcaatta aggcttgcac
      481 acgacctccg gaaaagccca aagcggccgg gtgactcccc tggccgtcat ttgtactttg
      541 ccgcataggg tttaagcggc ggtaaactta acaagctaaa tcaacgacat attaccaaaa
      601 tccccccctc tagcccgaca ttaagggttg ggggtatgat tccctgggga atggaaattt
      661 aatcttaacc atttccgttt agggtttgga catcggttaa ccacgacggc caaaacgcgt
      721 ctggcaaggg gaattaagga caatgcgcgt gctcctgatc gaagatgaca gcgccatggc
      781 tcgaagcatc gagctgatgc tgcgctcaga gggttttaac gtctacacga cggacctcgg
      841 cgaggaaggc atcgacctcg gaaagcttta tgattacgac atcatcacac tcgacctgtc
      901 gttgccggac atgagtgggt tcgaggtcct gaagtcgctg cggctggcca aggtccagac
      961 gccgatcctc attctctccg gcaatgccat cgtcgaagcg aaagtgaagg cgctcggctt
     1021 cggcgccgac gactacatga ccaagccgtt ccacaaggat gaactggtcg cgcgcatcca
     1081 ggcggttgtg cgccgctcca agggtcattc gcaatcggtc atcaccaccg gaaagctgac
     1141 ggtcaatctc gacgccaaga cggtggaggt cgacggttcg cgcgtgcatc tgaccggcaa
     1201 ggaatatcag atgctcgagc tgctttcgct tcgcaagggc acgaccctga ccaaggagat
     1261 gttcctgaac catctctatg gcggcatgga cgagcccgaa ctcaagatta tcgacgtctt
     1321 catctgcaaa ctgcgcaaaa agctcgcggt cgcgtgcaat ggggagcatt atatcgaaac
     1381 cgtctggggt cgcggctatg tgttgcgcga tcccgatgag aagattgccg cctgagattt
     1441 cctggttgtc gagggctgga agaggcgcgc gaaataccgc gcctcttttt tttgcttatc
     1501 cgaaggcggc tgcgcgcgac atttgggcgc gcgcggcttt cattgtgatg gctctgcccg
     1561 gacgctcgga cgcgaagctg ctgcccgatc ccagtgtggt ttccgccgaa cccaacacca
     1621 gatagccatc gtcggccaat gtgtccgaca ggcggtcgag gatgcccgtc ttggtggcgg
     1681 aatcgaaata catcaggaca ttgcggcaga agatcacatc gaaggggccg agcgacacaa
     1741 acggatccag aagattgagg acgcggaaca caacccggct gcggatgccg ctgcggatgc
     1801 gccattcctt cgcttggggc cggaaatgat tcgccagcat ctgcagcggc aggccgcgct
     1861 gcacctcacc atgggtgtaa agtcccgtct tggcgcgcgc gaccgcatcg gcactgacgt
     1921 cggtggcgag aatgtcgatg tcccatccgg cgaattgcgg cagacgcgcg aagatcatcg
     1981 caatcgaata gggttcctgg ccggtggcgc acgccgccga ccagatcgct atgcgccgtt
     2041 tggccatgcg ggaccgcaat agccgcggca gcaacgtgtc ttcgaaagat ttgaacgcgg
     2101 cggaatcgcg gaagaaagaa gtatcgcgga tggtcatcgc ttcgatcacc gcgcgcgtga
     2161 gcggttcatt gccgtatcga agatctgcga cgaggtggga cacgtccgcg aagccgtgca
     2221 gctccgccac cggctgaagc cggctcagga tgagattggc cctgtcttgc gtcaccacca
     2281 gcccggaggt tcgcttcatg agttccgcga ggaaccggaa atcctcctgc cgcaacggca
     2341 tgggtttaca tcaatcccgc ttcggcaaat ttcgcctcga cgatgtcctt gtcgaacggc
     2401 tttacgagat attcgttggc gcccgcgccg atggcatcgg tgatatgggc gatgtcgttt
     2461 tcggttgtgc aatagaccac aatcggcttg ccgccctcgg gctcgcgccg gagcgcgcgc
     2521 aggaactcca taccggacat ggccggcatg ttccaatcga gcaggatggc gtcgggcatg
     2581 ccgcgccggc agacatcgag cgccgcggcg ccgtcctcgg cttcctcgac ctcatattgg
     2641 agttcctgca gaatccggcg cgcgattttg cgcaagacgc ggctgtcgtc cacgatcagg
     2701 cattgcttca tgacatgttc cttcccgact ccttcgtcca tagtgacagg gccgggtaaa
     2761 cacgggctga actcggggag ccggcatcac ggtgcggcca cggatttttg cgaagacagc
     2821 cgcggagcta gcccgcggta aaggacactt cgttcggaag gcacgcgatg gtcagccggg
     2881 catcgagggt cttggcaagc tgataggtga gatagggctg gatcgagcgg ccatcgagca
     2941 aaccctccgg ttcgcccgaa agggcgcgct cgacttcgga catgagccgt accaccgtcc
     3001 cggtcccgat gaccctgaac gccggtgcct cgggagtgcc tgatgtttcg acgatgatac
     3061 gcccgccccg cggcaggcaa tcccctgtgg caagcacggc attcatgagg agcttggccc
     3121 agtccttggg ccaattggtg ggctcggcct gccacaaaag atcgattttg ccgcctttca
     3181 gcaagccgtt catgatgcgg cccacctcgc tcagatcgag ttccgcgccc gccgaacccg
     3241 cagccccgaa cgcgattcga cagaatttca gacgcgacgc cgcctgttcc gcgctggccg
     3301 ccaccaggcg cagcgcgtcc gcgcgcatgg caaggtcggt ctcgtcctcg agcacttcga
     3361 gcccgttgac cacggcgccc accgggctca caaggtcgtg gcatacgcgc gacaccagaa
     3421 gagcggcaaa atcgagatcg ttcatggcaa ggcctttggt gttcagcatg ggcagaaacg
     3481 acctttcgtt tgcagatcgt aaatgtgccc gcgccttctt aacgagtcct taaaacccgc
     3541 ctttatccac agagacatga ccgtcttcat atcggaattt acagccagac tcgcggaaat
     3601 tgcgcatgac gccgggcagg tgatccttaa gcattatgcc gccggcgtcc cgatccgctt
     3661 caagcaagac gcttcccccg tgacggccgc cgacgaagaa gccgaagcgc tcatcctgga
     3721 gcggctggcc gacgtcgcgc cgggcgtgcc catcgtcgcc gaggaggaaa tggcggcagg
     3781 ccgcaggccg cgccggctgg ggacgcgctt cttcctggtc gatccgctcg acgggacaaa
     3841 ggaattcctc aagcgcaatg gcgagttcac cgtgaacatc gcgttggtgg agcaaggccg
     3901 cccgatctta ggcgtcgtgc tggcgcctgc caaagcgcgg gcctttatcg gagatggcgt
     3961 caatggcgcg ttcgaattgg ccgcaccgga agatgcccaa ctggatatgg gccaggcccg
     4021 gcgcatcgcg gcgcgaaaag cgccagaggg cggtctgacc gtcattgcca gccgcaccca
     4081 tggcgagccc gcgacggagg aatatctgcg cagccatcgc atcgcccatc gcatgacggc
     4141 gggctcatcg ctgaaattct gtctggtggc ggcgggtgaa gccgatcttt atccgcgtct
     4201 ggggcgcacc atggagtggg acacagccgc cggccaagcg gtgctggaag cggctggcgg
     4261 ctcggtgaac acgctcgacg gcgcgccgct gcggtatggc aagatcgcgc aagcgttcgc
     4321 caacccccat tttgtggcgc gcggaatcag gtaacgggac agccgcgaga cttgatgaga
     4381 cgcgggcggt ggcgcaaaac tgtgccatcc cttgcaacag agaagtgaaa aaatgaccgg
     4441 attggaagtc aaaaactggt ttggcgatct gatctcacac cccaaaatca tcgtcgatgc
     4501 gaactctgtc caagacattg tcgcgatcct gaaggacaag gccaaatacc cgtcgccggt
     4561 gcgtgccgtc gggtccaatc attcgaccgc cccatgcggt gtggccgaag gcggcacgct
     4621 gatccggatg aaaatgaacc ggatcctcag catcggtgcc gattccatca cggtggaagc
     4681 gggtgcgcgg cacatcgaca tggccaagga gctccagaag cacaatctcc agttctatgt
     4741 gaacaccgag atcggcaacc tcacggcggg aagtgctgcc tgtgccggca ccaaggacgc
     4801 gtcttttccg ggcgaatacg gacaggtcgg gtcctatgtg atcggcatca aactcgttct
     4861 accgaacggc gatcttctgg aagtcaccga ggcggatccc ggactgatgc aacaggtgcg
     4921 ctccagctac gggaccttcg gcatcgtcta cgaagtcacc tatcggattc gcccgctgct
     4981 gcccatggcg gtgtaccacg agaccttcac gctggaagaa ttcgtcacga agctggatgc
     5041 gctgaaggca cgcggccagt cgatgatgtt ctattacttc cccttcgccg acaggatcac
     5101 gatcgaattc cgcagctaca atcccaacgc gacgggcagt cctaaccgct cggcctggac
     5161 tttgcgcaat tatttgtggg gcaccgcggg accgcgcttc ggtcatgata tcgaggccac
     5221 gattcctgtt cccgcggtcc gctatgaggt gatcgacggc ttcaacgcca tcctgcgctt
     5281 caagctcgaa cacctgatcc gggccgacta cacgcttgcg cccgatcaaa tcatcaacta
     5341 cccggacgta tcggataaca gccgctacac cttcagcctg ttcgccttcc ccgaaacgca
     5401 atttccgcag acgctttcgg agttcttcaa attcgcgcgc gattactatg acaaacaggg
     5461 ctatcgatcg aatttgctgt atgtcggcta tcacatcgcg caagaccaac agtcgctgct
     5521 gtcctattcc tacgatgggc cggtgatgac gatcgacccg gtatcgaccg gcaataatgg
     5581 ctggaaaccg ttcctcgaaa cctacaatca attctgcatc gcgcgcggcg gcaagccact
     5641 gttcaatcag acttatggcc tgaccgcgga catcgtacag aaagcgttcg gcgagcggct
     5701 gcggacgttc gccggaacgc gccggaaatt cgatcccgat ggccggctgt tgaacgatta
     5761 tttccggatg ctgctcagcg cgtagcggcg ctttttgcta ggattggccg ccgcgcttgg
     5821 tgatgacggc gctggtgatg atattgtctc cttcgcgcaa agaaccggag accacttggg
     5881 tcacacgccc gtcgctgagg ccaagtgcga tgtcgcgagg ctcaggctta ccgttcgcca
     5941 atacccagac gcgcccaaaa tgcgcggcac ccgccggctg cttcatgggg gcaaaccggg
     6001 attcgatttt cggaggcggc gcgaatgcga gcgcgccatt ggggacggtc agcacattct
     6061 tcgactctgc ggcgataatg gcgatactgg cgctcatgcc cggccgcagc atatcgcccg
     6121 gattggcggc ggcgagcgtt cctcgatagg tgacgacctg cctgccgtct ctgatctgcc
     6181 tttccggcca gagatccagc gccgtcaaag ccgcggagaa tgtgcgttgc ggatacgccg
     6241 gcacggtgaa accggcccgc tcgccggccc gcaaggcacc gagtgcgttc tcgggaaacg
     6301 ccgtcgtcac gttgagttcc gaaagatcgg cgaccattgt gagaagcggc tgaccacgag
     6361 aaaccgcacg gacatcgctt gaaggatcat agccgatctt caacaccgtg ccgtcgaagg
     6421 gtgcgcgaat gtcgagactg gcgagaacgt tacgcgagga cgcaacgctc tgctgcgcgg
     6481 cctgaaccaa tttgcgcgct tcatcgccat tggccgagac ccgcgcgagg cgcgcttcag
     6541 cggcatcggc cgtgccggga cgcatatcgt tgcgagcacg cataagcgcc gcgcgcgctt
     6601 cggcgatgtc gatttgggag cgcgccaaat ccgcctgccg cgcagccaac tcagcttgta
     6661 ccgacagcag atcggcgcgc gccgtttccg acaccagacg cgccaggact tggcccttgg
     6721 agacacggtc tccgtttttg acggccaagc tctggagccg gccgctcgct ggcgccacga
     6781 tgtccacggg attgcgcggt tcaagcgtgc ctgtgctgtg cacagtcgcc tccaccgtgg
     6841 tccgggcagc tttcgcaaca ccgtagacga tggtgggctg gctgctgcca aacggccacc
     6901 acagaatcat accaagcaac aggacggtga aaacgatggc ggcggcaaga cgacgccgcg
     6961 tccaatatgc gcgccgcaag gggtttgctt cggccggtgc aggcgcggat gtttcggttc
     7021 cgccttcggc ttgacgggat tccggtttga ctggttgcgg tctttccttc tgacgcggtt
     7081 cagatgtggg ctgacgcggt tcggatgatg gccgttcctg tgacggctga cggagtgtgg
     7141 cttcgccggg ctgcggtaat ccttgcacat ccgatccgcc agaaaccggc cgcggggatc
     7201 gctcattggg cgggggtatg tgcttgttca tgacttacct cagtggcgtc ctaccttcct
     7261 cggccaaaca aattcgccga ttcctcgcct tgctgagata acgcatcaac gcccgatcgg
     7321 ttacggccca agctccggct tggaaacctc agccgcatta accaaagacg gatttagcgg
     7381 tcgtagcctt tgcgattgga ccagaagagc agccccatca ggccgccccc aagaaccatc
     7441 gaaagcacaa cgcccagtcc caaggcgata tagccattgg tgtcgatcgg cgcgtccgtc
     7501 agatgccagc cgtagaacag gaaccagatc gcagcgccga gcaggatgcc catggcgaga
     7561 atcgcaatcc agccgccggc gccgaggggg ctacgttttg gtccgtgtac ggaattattt
     7621 gccatggtgg atgtcccggt gaattggttc ggctggcctt ggcgttcggt tggcctgttt
     7681 cggttggcct ggcggcaaag ttttgcgatc aaacgccaaa gtttgccata gatttccgtt
     7741 cttcgtgcgc aaaaaggctg accatcccgc cttccgcctg cctcaaaaac gcccaaactg
     7801 ctatattgaa tggagattcg cgaaagcgcg ggcgcaggga aggaacccag gatgagtatt
     7861 ttcaagatat tgcgcgcggg ccttggcgcg attgtcctgt cattcgccct tgcggcgacg
     7921 gcatcggccg ctccggcgcg cgaccctcgc gatccggaca cctatagccc ggaagaggtc
     7981 gtcaaggcgg gcgccgattt cttcggcgtc accaccgagg ccatggccaa ggcggtcgag
     8041 aaggttttgt cccgctacgg ccggcccaat gcctacatcg ccggcagtga gggttccggc
     8101 gcggttgtcg tgggcctgcg ctatggcgag ggcgaactgt acatgaaggg caagggcgcg
     8161 ccggaaaaag tgttctggca ggggccttcg atcgggttcg accttggcgc caacgcctcg
     8221 aagaccttca cgctggtcta caatctgccc agcccggaat cgattttcga gcgctttccg
     8281 ggcgtcgaag gcagcgccta tttcatcgcc ggaatcggcg tgaattatca gcagaacggc
     8341 aaaatcatcc tggcgccgat gcgcaccgga gtgggcctgc gctacgccgc gaatatcggc
     8401 tatctgtcct attccaagga acggaactgg gtgccgtttt agacgcagcg cggcttaaaa
     8461 atggacgtgg tgagcgcgga tgatgaagga aagtccatgc gcgacaccga agatcaccag
     8521 tcctagaagg atcgagccca gcaagccgat gcccgaacca atcaggatgg caaggccatc
     8581 gcgttcgatc aggccaaggc cgatcagcac caatcccagg gctggcaaat tgtggccgaa
     8641 gggaatggga agcgtcgata ccatcgcgag gatgatcgcg atgattccga tgaagcgttc
     8701 ggcccgctgg cccgtcaccc attccatgcg cggcgtcagc agccgctcga accaggaaag
     8761 caccgcggcc acgcgcggcg ccagcgcctt cagccaatgg gtgccgattt tccagcggcc
     8821 cataaagccc ggcaaaatca gatgctccac gccaagcaga agctgaaccg caaagatcag
     8881 gggcggaatg ccggtcgcga cattgccgaa gggcaccgtc gagggaagga tgttgggcag
     8941 cgcgaaaatc gcgatcagga cgcccaggcc gcgatctccg agccccgaaa cgatttccgc
     9001 cagcgtgacg cggtccgaat gaagcgaatt cgcaaattcg cgcaacagct ccgacgttcg
     9061 gattggcctg ggactgcctt ccgccgctac ggcatcgacc tgttgcgtca tcgtcatctc
     9121 gcgcgggcgc tagccgtgac gggcgccgcc gctgtcggtc cgtcgctccc ccccgtcacc
     9181 gcgggcagat agatcagcaa agccgaagcg acatagatcg aggagaaggt tccaaccacg
     9241 atcccgaaca ggatcgcggc ggtgaaatcg aacaacgtcg gcccgccgaa cagaagcagc
     9301 ggaatgatcg agatggctgt cgcgcccgag gtcagcacgg tgcgagacag cgtctgattg
     9361 gtcgagatat tgatgagttc cttgagccct atgcgcttgt atcgccgccg gttttcgcgg
     9421 atacggtcga acaccaccac cgtgtcgttc accgaatagc ccgcaagcgt cagcagcgcc
     9481 gccaccgagg tgagcgtgaa atccagatgc atcagcgaaa agagtcccgc cgtcacgaac
     9541 acgtcgtggc cggtggcgat aatggcgctc accccatatt gccattcgaa acgcacagca
     9601 acgtagaggc cgatcatgaa gacggcgatc acggtggcaa gaatgcccgc attcctcagc
     9661 tcgtccgaaa cctttggccc gaccacttcg gtgcgccgga aggcatatcc ctcacccagc
     9721 gcggttcggg tcgtgttcac aaccgcctga tcgctggccg gcgcattcac ctccggctga
     9781 acgcggatga gaatgcagga attgaccggc atctcgcatt cgccgccacc gaaatattgc
     9841 aactgcgctt ccgcgaaatg caggttgtcg accttcgtgc gcatggcgcc gatatcgatg
     9901 ctctgggcgg ctttcacttc catcaacacg ccgccggtga aatcgatgcc gaaattcagc
     9961 ccgtgcacca gcagtgagat cagtgcggcc aggaccagca gcccatcgat ggcgaaggca
    10021 tagaagcgcg ctccgacgaa gtcgatattg gtgtggtccg gaatcagttt gagaaggcgc
    10081 atgcgttgga agatccggcg aaaatgcccc tgcggggggt gagacatagc ggcaaagccc
    10141 ggccaagcca agggaaacgg gtccggcatg tcacccagtg taacagcccg ggctttcgga
    10201 accctaaaca ggaatgcgat tgcaggcagg cttgacgcga atgttat
//
LOCUS       DASYEG010000327        11178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_854056, whole genome shotgun sequence.
ACCESSION   DASYEG010000327 DASYEG010000000
VERSION     DASYEG010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 11178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11178
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854056"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /gene="hisD"
                     /locus_tag="VGM72_13780"
     CDS             complement(<1..1157)
                     /gene="hisD"
                     /locus_tag="VGM72_13780"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY4266383.1"
                     /translation="MTMLRRLNASDEDFEVRFAALVDANREAEEDVSRSVTRIIADVR
                     ARGDAALIELSRRFDRVELSSGTLRVSVEEIAAAERGCAPDLKAALHFAAARIEAYHR
                     RQIPADENFTDETGATLGWRWSPLASVGLYVPGGTASYPSSVLMNAIPARVARVERVV
                     MVSPASGGAIPPLTLVAAKIAGVSEIYRIGGAQAVAALAYGTATIAPVDKIVGPGNAY
                     VAAAKREVFGRVGIDSIAGPSEILVVADGTNDPAWIAADLLSQAEHDASSQSILITDD
                     AGFADLVAREVERQLALLPRRDIAERAWNAHGAIIVVARLDDAAHLVNRIAPEHLEIA
                     TDDPRAFFARVKHAGAVFLGRHTPEAVGDYIAGPNHVLPTSRTARFSSGLSV"
     gene            1626..1925
                     /locus_tag="VGM72_13785"
     CDS             1626..1925
                     /locus_tag="VGM72_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266384.1"
                     /translation="MPSPPSATPVAEATITDSAPLTTEIVDELSARLAGGDAVALRDP
                     DNKVFAVLLSPPKFDLLQVLSDLAGSGRLVLEPKQTGDSTNVPTSLVEYIERLEK"
     gene            1922..2254
                     /locus_tag="VGM72_13790"
     CDS             1922..2254
                     /locus_tag="VGM72_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266385.1"
                     /translation="MTRAPIGPLEVSFDDDEVGLYLHDHATPQEKECLSTFLRVLELT
                     EMECNVAIITQPGKGYPGGVVYLIATCGDVLELWLETNQSTRASCRGISRGSLARMDV
                     TIWGGHDG"
     gene            2247..2564
                     /locus_tag="VGM72_13795"
     CDS             2247..2564
                     /locus_tag="VGM72_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266386.1"
                     /translation="MADFFYRHWAELLKIVGAACGMAGTVLMANRYVKTTKFWRVVRG
                     LVFALVRGEAARSIAGVADIKTEKPLPSLQGLAFIAFGFFLQLIPDFIALFASGSGAA
                     GAQ"
     gene            2702..3346
                     /locus_tag="VGM72_13800"
     CDS             2702..3346
                     /locus_tag="VGM72_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HEY4266387.1"
                     /translation="MLRWFQALMPREDRFFDLYNRHAQTLVQGAEALRELLNGGAGVS
                     EAARKLFGFEEQADAVARDVLVLVRRTFITPFDRSDIKDLINTLDDTIDQMKKTAKTI
                     LLFEVDTLEPEMAEMGERILQAAKLTVEAVKLLGSLRENATRLNAITEDIIRLEDDTD
                     VLNDLGIKALFKRHRNGNAMDYIVGIEIYNHLERVMDRFEDVAHRISGIVIEQV"
     gene            3353..4339
                     /locus_tag="VGM72_13805"
     CDS             3353..4339
                     /locus_tag="VGM72_13805"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEY4266388.1"
                     /translation="MFLLAGLIAIALAFDFLNGRNDAANSIATIVSTRVLSPRYAVAW
                     AAFFNFIAFLVFGLSVASTVGQGIVAADAISDRVIFGALLGAISWNITTGYLGIPSSS
                     SHALIGGLVGAGVAHVGIRAIVWNGLGKTAGAIVMSPMIGLALALMLVLIVSWSFARA
                     VPAHVDSIFRRAQFVSASLYSLGHGGNDAQKTMGIIAALLTAHSAVPVAFHVPLWVVI
                     ACNGAMALGTLLGGWRIVRTMGSRITRLTPVQGVCAETAGSITLFIATGLGIPVSTTH
                     TITGAIVGVGAARRASAVRWNVAQRIVVAWFITMPAAAFVGAGFYELASLIG"
     gene            4370..4828
                     /locus_tag="VGM72_13810"
     CDS             4370..4828
                     /locus_tag="VGM72_13810"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY4266389.1"
                     /translation="MNRTAAHVANVQYAALPWRRVQGALEILLITTRNTRSWIVPKGW
                     PLVGCTPSECAANEALEEAGVLGEVAEKALGVFHYNKRQKSGDIVPCKVHVFAMEVVH
                     QRRSWAEKAARDICWCSLPEALARVTEPSLRRLIAKFAKTFGGPGERPTA"
     gene            complement(4862..5749)
                     /locus_tag="VGM72_13815"
     CDS             complement(4862..5749)
                     /locus_tag="VGM72_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266390.1"
                     /translation="MRAYRLMRLSLGGVLICSLALAARAEQAGPTAIELAGQWSAWQD
                     HLRSAMDGCVSANEKSCLSKAFKDLPRPPIDNPAHSVSGELFYDLRTAQAMLAVPSIA
                     DALWREYGVDSVAYLGTGYSVPLTPAGVASYWQGTQREYFVPNLCAQTADSALCPAAD
                     PDVWTWRLTSGQMKAALDRPISAIMKGDTRKLRSRMRAAPRDDGLPNAFVRFALLNPG
                     NYKGTFGRADAKRVFFADFAQVSGKSLRQALIATGASTLIGDPDPGQVFFIWIYAPGG
                     NTKAAAASWRAVFDALEAD"
     gene            5905..6768
                     /locus_tag="VGM72_13820"
     CDS             5905..6768
                     /locus_tag="VGM72_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266391.1"
                     /translation="MFNSTILDVVAGLLFTFLTVSLVASTVTEGLASLLGWRAQMLLR
                     GVKSLLNDPHFTGLAKDLYNHALVNSQASGTAQTENALAVKPSYIEPQHFAGALIDLM
                     EKLPATRTQTQTQIQAAILQIPNPQLQQTLQFFYTRAGADITTFQNEIATWFDSSMSR
                     LSGLYKRWMQICSFAVGLGVAVVLNVDALHVAQALWVQPAIIQKFAPKEGINTQEALD
                     ALYNANLPIGWSQQTARASFASPLAIASLMGGWLITAFATLFGAPFWFDALQKITNLR
                     GAGPKPNEKKR"
     gene            complement(6789..7226)
                     /locus_tag="VGM72_13825"
     CDS             complement(6789..7226)
                     /locus_tag="VGM72_13825"
                     /inference="COORDINATES: protein motif:HMM:NF022612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2948 family protein"
                     /protein_id="HEY4266392.1"
                     /translation="MTALKLLAEDEEDLKIISAHLQDAVLRVGDLLYLPSQHRFVLVL
                     NRFCWEDCPDAGIGARVLTGLHFDSVLKVQSQNVRQGDPDAVAELLAIHFTPADEGGG
                     FIDLMLAGGGRVRLHVECIEAAMRDMTEPRPAVARPEHDLERS"
     gene            complement(7223..8503)
                     /gene="murA"
                     /locus_tag="VGM72_13830"
     CDS             complement(7223..8503)
                     /gene="murA"
                     /locus_tag="VGM72_13830"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY4266393.1"
                     /translation="MDRIRITGGRRLKGEIPISGAKNAALPLMAACLLSEQPLTLTNV
                     PNLADVVYMADVLRQLGVGVAWQMSGVDHVGGTIKLHAPKRPQSLATYDAVRKMRASF
                     LVLGPLVARIREAMVSLPGGCAIGARPVDLHLKGLAAMGAEIELTGGYVLARAPGGLK
                     GAEFVFPSVSVGATENLLMAASLAKGTTVLKNAAREPEIGDLGQCLIAMGAKISGLGT
                     STIVVEGVPELGPATHAVLPDRIETGTYAIAAAITGGEVELTGTTSELVGALIPLLRS
                     TGAHVEDTSFGFSVARNGGGIKAADVATEPFPGFPTDLQAQYMALMTVAEGTSHIRET
                     IFENRFMHVPELLRMGAEIKVEGETAIVTGHRELKGAPVMATDLRASVSLILAGLAAE
                     GETIVNRVYHLDRGFERIEDKLKACGADIERLKQ"
     gene            8868..8942
                     /locus_tag="VGM72_13835"
     tRNA            8868..8942
                     /locus_tag="VGM72_13835"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8900..8902,aa:Thr,seq:ggt)
     gene            9026..9193
                     /locus_tag="VGM72_13840"
     CDS             9026..9193
                     /locus_tag="VGM72_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HEY4266394.1"
                     /translation="MPIVHIDVLTIQPLVALLFGILVLLIPRLLNYLVAIYLILIGII
                     GLWPQLFTHVT"
     gene            complement(9203..>11178)
                     /locus_tag="VGM72_13845"
     CDS             complement(9203..>11178)
                     /locus_tag="VGM72_13845"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEY4266395.1"
                     /translation="IRKGWHTYWRNPGDAGFPTTAKWQLPEGWKAGDIQWPYPKRLPL
                     EQLMTYGYQDEVSLPVDISVPADAKPGEGAIVSADLMYLVCTDASGVCVPEESHVSVP
                     LGIAAQTPPPDGKTAAFFADARSKLAHASPWKAVYDAGDKRFALLIESPELVSARPRD
                     VAFYPYTDGYVQAGAPQRLGTSDKGFVIEGVTDRKLATQEKRASAGKIGGVLVLTGAD
                     GRVDALDVDAEPGPVPASSVTLLQNTSNEVGFAAALLFAVLGGLILNLMPCVFPVLSM
                     KALALAAKREAPHRAKAAALAYGAGVLVSFLALGGALVVLRGGSAGLGWGFQLQQPLF
                     VSALALLMFAVGLNLSGLYEIGGGRLSGMGGRLAGKEGAAGSFFTGVLAVVVATPCTA
                     PFMGAAMGYALTQNAGVALAIFAGLGVGFAAPFMVLGLWPRALGVLPRPGAWMTTLRQ
                     VLAFPMYGAAIWLVWVLSLQAGSDGVLAVLAGALALAFGLWIYGRSQAAHGTGRTLGF
                     AAAAVAILAAIVLIPLTGVGARPAGTAGVGLEGGGLAYEPFSASRLQSLRQEGRPVFI
                     NATAAWCITCLVNERVALSGSRLGEAFAARKVAALKADWTTQDSAITALLASQGRSGV
                     PLYLYYAPGAEKPAVLPQLLTESTVMAALDNAQ"
BASE COUNT         2080 a         3613 c         3425 g         2060 t
ORIGIN      
        1 accgaaagcc ccgacgaaaa ccgcgccgtg cgcgaggtag gcagcacatg gttaggcccg
       61 gcgatgtaat cgccaaccgc ttcgggcgtg tgccgtccga ggaaaaccgc gcccgcatgt
      121 ttcacgcgcg cgaaaaatgc cctgggatcg tccgtggcga tctccaaatg ctcgggcgcg
      181 atgcggttca ccaagtgcgc cgcatcgtcc agccgcgcca ccacgatgat cgcgccatgc
      241 gcgttccacg cgcgctcggc gatgtcacgg cgcggcagca atgcgagctg gcgctcgacc
      301 tcgcgcgcga ccaaatcggc gaaccccgca tcgtcggtga taaggatgga ttgcgaggag
      361 gcatcgtgtt ccgcctggga gagaagatcg gcggcgatcc aggccggatc gttcgtccca
      421 tccgcgacca cgagaatctc cgacggcccg gcgatggaat cgatgccgac gcggccgaag
      481 acctcgcgct tggcggccgc cacataggcg ttgcccggtc ccacgatctt gtcgacgggg
      541 gcaatcgtcg cggtgccata ggccaaagcg gcgaccgctt gcgccccgcc aatgcggtag
      601 atctccgata cgcccgcgat cttagccgcc accagtgtca gcggaggaat cgcgcctccc
      661 gaagcggggc tcaccatgac gacgcgctcc acacgcgcca cgcgcgcagg gatcgcgttc
      721 atcaagaccg aactcggata gctggccgtt ccgcccggca cgtaaagtcc gacgctggcg
      781 agaggactcc agcgccagcc cagtgtcgcg ccggtctcgt cggtgaagtt ctcgtccgca
      841 gggatctggc gtctgtgata ggcctcgatg cgggcggccg cgaaatgcag ggccgctttc
      901 aggtcgggcg cgcagccgcg ttccgccgcc gcgatctctt cgaccgaaac gcgcaaggtt
      961 ccggatgaga gttccacgcg gtcgaaacgg cgcgacaatt cgatcagggc cgcatcgccc
     1021 cgcgcgcgca catcggcgat gatccgcgtc acgctgcgcg aaacatcctc ttcagcctcg
     1081 cgattcgcgt cgacgagcgc ggcgaaacgc acctcgaaat cctcatcgct tgcgttcaat
     1141 cggcgcaaca tcgtcatcct gtcatcgtct gcccgcgggc aaccggcgtt gatcttgagc
     1201 taatggattt gctcgcgtgc gggaatgcca attttgccgc caacggcctg gccggaaaac
     1261 cgtcctgccc ccctcccccc tcttttggca gatgaagacg gaaattttcc agggattgcg
     1321 cggaaaaatt acagtgcagg gtaattttat taggtgcgaa tagaggtgca atccggggca
     1381 tgctgtgggt tggtggggag gagccgctga ggtcgtcatc ccctggatat aggaacaaga
     1441 tggcgttcgg ccatacggac tcgataaacc gccgcaacat tccgttcggg ccgttcatgg
     1501 cgtcgaggaa actactcaat gatctcccac ggcgctatcc tcccgctcac gcggttgacg
     1561 aaccgggcgg ttgcgggaat gccgaacgaa tcctcataag ttttcatttc gtggagtccg
     1621 agcaaatgcc tagtccgcca tctgcaacgc cggtcgcaga agcaaccatc accgatagcg
     1681 caccgctgac aacggaaatc gtggatgaac tcagcgcgcg tttggccggc ggcgatgccg
     1741 tcgccctgcg cgatcccgac aacaaggtct ttgcggtttt gctgtctccc cccaaattcg
     1801 atctgctgca ggtcttatcg gatttggcgg gcagcggacg cttggtcctg gagccgaaac
     1861 agaccggcga ctccacgaat gtcccaacga gtctcgttga atacatcgag cgcttggaaa
     1921 aatgacacgc gcgccaatcg gtccgttgga ggttagcttc gacgacgacg aggtcgggct
     1981 gtatctgcac gaccatgcca cgccccagga gaaggagtgt ttgtcgacat tcctgcgggt
     2041 tctcgagttg accgagatgg aatgcaatgt cgcgatcatc acccagcccg gtaagggata
     2101 tcccggcggc gtggtttatc tcatcgcgac ctgtggtgac gttctcgaac tgtggctgga
     2161 aacaaaccag agcacgcgtg ccagttgccg gggcatcagc agaggcagcc tcgcccgaat
     2221 ggatgtaaca atatggggag gacacgatgg ctgatttctt ctatcggcat tgggccgaac
     2281 tgctgaaaat cgtgggcgcg gcgtgcggca tggcgggaac cgtgctcatg gcaaacagat
     2341 atgtcaaaac cacgaagttc tggagggtcg tgcgcggtct ggtctttgca ttggtgaggg
     2401 gagaggccgc ccgcagcata gcgggcgtcg ccgacatcaa gacagaaaaa cccttgccct
     2461 ccctccaggg gctggccttt atcgcgtttg gatttttcct gcagctgata cccgacttca
     2521 ttgccctctt cgccagcggc tcgggagccg cgggcgctca atagggacgc ccccgctccg
     2581 gccgcgtttt ggacgccggg tgtaaaatcg ccttttgacg gctgcgtctc tcataacctc
     2641 ttgcaaagcc agagccagtt tgaccttagt cgggttctac ttttctcctc ccgaggttcc
     2701 catgctccgc tggttccagg ccctcatgcc gcgcgaggac cgtttcttcg atctctacaa
     2761 tcgtcacgcg cagaccctgg tccagggcgc agaagccctg cgcgagctgc tcaacggcgg
     2821 cgcaggcgtg agcgaggcgg cccgcaagct ttttggtttc gaggaacagg ccgatgccgt
     2881 cgcgcgcgac gtgctcgtcc tggtgcgccg gaccttcatc acgccgttcg accgcagcga
     2941 catcaaggat ctgatcaaca cgctcgacga cacgatcgat cagatgaaaa agaccgcgaa
     3001 gaccatcctg ttgttcgaag tcgatacgct ggaaccggaa atggcggaaa tgggagagcg
     3061 catcctgcag gccgcgaaac tcacggtcga ggcggtcaag ctgctcggat cgctccgcga
     3121 gaacgcgacg cggctaaacg cgatcaccga ggacatcata cgtctcgaag acgataccga
     3181 cgtcctgaac gatttgggga tcaaggccct gttcaaaagg caccgcaacg gcaacgccat
     3241 ggactatatc gttggaatcg agatctacaa tcatctggaa cgggtcatgg atcgtttcga
     3301 ggacgtggct caccgcatca gcggcatcgt gatcgagcag gtctgactct tcatgtttct
     3361 gctcgccgga ctcatcgcga tcgcgcttgc cttcgatttc ctgaacggac gcaacgacgc
     3421 ggccaattcc atcgcgacca tcgtgtcgac ccgcgtgttg tcgccccgct atgcggtggc
     3481 gtgggcggca ttcttcaact tcatcgcatt tctcgttttc ggcctgagcg tggccagcac
     3541 ggtcggccag ggcatcgtcg cagcggacgc gatttccgac cgggtcatct tcggtgccct
     3601 tttgggagcc atctcctgga acatcaccac cggatatctg ggcattccgt caagcagctc
     3661 gcatgcgctg atcggcggcc tggtcggcgc gggcgtggcg catgtcggca ttcgggccat
     3721 cgtttggaac ggcctcggca agaccgccgg tgcgatcgtc atgtcgccca tgataggcct
     3781 tgcgctcgca ctgatgctgg tcctgatcgt ttcctggtcg ttcgcccgcg cggtgccggc
     3841 gcatgtcgac agcatttttc gacgcgcgca gttcgtctcg gcctcgcttt attcgctcgg
     3901 ccacggcggc aacgacgcgc aaaagaccat ggggatcatc gcggcgcttc tgaccgctca
     3961 ttcggcggtt ccggtggcct tccacgttcc tctttgggtg gtcatcgctt gtaacggagc
     4021 gatggcgtta ggcacgctgc ttggcggttg gcggatcgtg cgtacaatgg gatcgcgaat
     4081 cacgcggctt acgccggtgc aaggcgtctg cgcggaaact gcagggtcga taacattgtt
     4141 catcgcgacc ggtcttggca ttcccgtatc gaccacccac acgatcaccg gggcgattgt
     4201 cggcgttggg gctgcgcgcc gcgcctcggc cgtgcgctgg aacgtggcgc agcgcattgt
     4261 cgttgcctgg ttcatcacca tgccggcggc cgcctttgtc ggagcgggat tttatgaact
     4321 ggcgagcctc atcggatagg cgaaacgcca tgattggaga tggtttgccg tgaaccggac
     4381 tgccgcgcat gtcgccaatg ttcaatacgc ggcactgcca tggcgccggg tccaaggcgc
     4441 ccttgaaatt ctgctcatca ccacgcgcaa cacccgaagc tggatcgtgc cgaaaggctg
     4501 gccgctcgtc ggctgcacac ccagcgaatg cgccgcaaac gaggccctgg aagaggcggg
     4561 ggtcctgggc gaagtagcgg agaaggcgct cggtgtcttc cactacaata agcgccagaa
     4621 atccggcgat atcgttccct gcaaagttca tgtgttcgcg atggaagtcg tgcaccagcg
     4681 ccgtagctgg gccgaaaagg ccgcgcgcga catctgctgg tgttcgctgc cggaagcgct
     4741 cgcgcgcgtg acggagccaa gcctgcggcg gctcatcgcg aaattcgcaa aaacattcgg
     4801 cggaccgggt gaacgcccca cagcgtagaa tttcccaacc cgattctaaa acagaccggg
     4861 ttcaatccgc ttcaagcgcg tcgaaaacag cgcgccagct tgccgccgcg gccttggtgt
     4921 tcccgccggg cgcgtaaatc cagatgaaga acacctggcc gggatcggga tcgccgatca
     4981 gcgtggaggc gcccgtggca atcagggctt gccgcaggga tttgccgctc acctgcgcaa
     5041 aatccgcgaa gaacacgcgc ttggcatcgg cccgtccaaa cgtgcccttg taattgccgg
     5101 gattcagcag ggcgaaacga acgaatgcgt tgggcaagcc gtcgtcacgg ggtgcggccc
     5161 gcatgcggct tcttaacttg cgcgtatcgc ctttcatgat cgcgctgatc ggcctgtcga
     5221 gtgcggcctt catctggccg gaggtcagac gccaggtcca cacatcggga tcggccgcag
     5281 ggcacaacgc ggaatcggcc gtctgcgcgc aaagatttgg cacgaaatat tcgcgctgcg
     5341 ttccctgcca ataggaggcc acccccgccg gcgtcagcgg cacggaatat cccgtgccca
     5401 gataagccac cgagtcgacg ccatattccc gccagagcgc atcggcgatg ctgggaacgg
     5461 cgagcatcgc ctgggccgtg cgcagatcgt agaacagctc gcccgaaacc gagtgcgccg
     5521 gattgtcgat tggcggccgc ggtaaatcct tgaacgcttt cgacaggcac gatttttcgt
     5581 tcgcggagac acagccgtcc atcgcggatc gcagatgatc ctgccacgcg ctccattgcc
     5641 cggccagttc tatcgccgtc gggcccgcct gctccgcgcg cgccgccaac gcgagagagc
     5701 agatcaggac gccgccaagc gacaatcgca tcaatctgta cgctctcatg gctttccccg
     5761 gccggctggc gcattttgtg gcattgccgc ggtcttgcgt ccagaatttc ctctttcatt
     5821 tcgccctgga ctattgtgtc ctggcagggg gcatttcatt caataccgcg cgcgaatccg
     5881 gttgcgcacg acagggggag agcgatgttc aacagcacga ttctcgatgt ggtcgcaggc
     5941 ttgctgttta cgtttctcac cgtcagcctt gtcgcgagca ccgtgacgga aggattggct
     6001 tcgctgctgg gttggcgggc gcagatgctg ctccgaggcg tgaaatccct gctgaacgat
     6061 ccccacttca ccggcttggc caaggatctt tacaatcatg cgcttgtcaa ttcacaggcg
     6121 tccggcacag cgcaaaccga gaacgccctg gcggtgaagc cgtcctacat cgagccgcag
     6181 catttcgcgg gcgcgctcat cgatctcatg gagaagctgc cggccacgcg gacgcaaacg
     6241 caaacgcaga tccaggccgc catcctgcaa atccccaacc cgcaactcca gcagaccttg
     6301 cagttcttct atacgcgagc cggggccgac atcacgacct tccaaaacga gatcgccacc
     6361 tggttcgact catcgatgag ccggctgagc ggtctctaca aacgctggat gcaaatctgc
     6421 tccttcgcgg tcggtctcgg cgtggcggtg gtgctgaatg tcgatgcgct tcatgtcgct
     6481 caggcgctct gggttcagcc cgccattatt cagaaattcg cgccaaagga gggaatcaac
     6541 acgcaggagg cgctggacgc gctctataac gccaatcttc ccatcggttg gagccagcag
     6601 acggcgagag cgagtttcgc atcgcccctt gccatcgctt cgctcatggg cggttggctg
     6661 atcaccgctt tcgcaaccct gttcggagcg ccgttctggt tcgatgccct gcagaagatc
     6721 accaatctgc gcggcgcggg gccaaagccc aacgaaaaga agcgttagcc acttcgcccg
     6781 atcgcgcgtt agctacgttc cagatcatgt tcgggccgcg cgaccgcggg gcgaggttct
     6841 gtcatgtcgc gcatcgccgc ctcgatacat tccacgtgaa gcctgacgcg cccgccgccc
     6901 gccagcatca ggtcgatgaa cccgccgcct tcgtccgcgg gcgtgaaatg gatggcgagg
     6961 agttcggcca cggcgtcagg atcgccctgc cgcacattct gcgactgtac cttcagcacg
     7021 ctgtcgaaat gcaggccggt cagcacgcgc gcgccgatcc cggcatcggg acaatcttcc
     7081 cagcagaagc ggttcagcac cagcacgaag cgatgctggc tgggcagata gaggaggtcg
     7141 cccacgcgaa gcaccgcatc ctgcagatgc gcggaaatga tcttcagatc ctcctcgtcc
     7201 tcggcgagca gtttcaatgc cgtcattgct tcagccgctc gatgtccgcg ccgcaggctt
     7261 tcagtttatc ttcgatgcgt tcgaagccgc ggtcgagatg atagacgcga ttgacaatgg
     7321 tttcgccctc cgccgcaagg cccgcaagga tgagactaac cgaggcgcgc aaatccgtgg
     7381 ccataacggg tgcgcctttc agctccctgt ggccggtgac gatcgcggtc tcgccttcca
     7441 ccttgatctc ggcccccatg cgcaagagtt ccggcacatg catgaagcga ttctcgaaaa
     7501 tagtttcgcg aatatgcgaa gtgccttcgg cgaccgtcat cagcgccatg tattgcgcct
     7561 gcagatcggt gggaaaaccc ggaaacggtt cggtggcgac gtctgcggct ttgatgccgc
     7621 cgccattgcg cgccaccgaa aagccgaagc tcgtgtcttc cacatgcgcg ccggtactgc
     7681 gcagcagcgg gatgagcgca ccgaccagct ccgatgtcgt tccggtcagc tccacctcgc
     7741 cgccggtgat cgccgccgca atcgcatagg tgccggtctc gatgcggtcg ggcagcacgg
     7801 catgggtggc cggccccagc tcgggcacgc cttccaccac gatggtcgag gttccgagac
     7861 cggaaatctt cgcccccatg gcgatcagac actgaccgag atcgccgatc tcgggttcgc
     7921 gcgcggcgtt cttcagcacg gttgtgccct tggcgaggct cgccgccatc agaagatttt
     7981 cggtcgcgcc cacggacacc gacgggaaga cgaactccgc gcctttgaga ccgcccggcg
     8041 cgcgcgccag cacatagccg ccggtcagtt cgatctccgc gcccatggcc gccagaccct
     8101 tcagatgcag atccaccggc cgcgcgccga tggcgcaacc gccgggcaag gacaccatcg
     8161 cctcgcggat gcgcgcgacc agcggtccaa gcaccaggaa gctcgcgcgc atcttgcgca
     8221 cggcatcgta ggtggccagg ctctgcgggc gtttgggcgc gtgcagcttg atcgtgccgc
     8281 cgacatgatc gacgccgctc atctgccacg cgacgccgac gccgagctga cgcagcacat
     8341 cggccatata gacgacatcg gcgagattgg ggacattggt gagggtgagc ggctgttcgg
     8401 acaaaaggca cgccgccatc agcggcagcg ctgcgttttt ggcgccgctg atgggaattt
     8461 cccctttgag gcggcggccg ccggtgatgc gaatgcgatc catggagctc tatgtggcct
     8521 ttcgcggtgt ctcattccag caggaagcac aaatatggcc agcggggcgc gcaaatatgg
     8581 gcgcggggat tgttattgtc gctttggggc ggatttcggc ctggtttcgc ttgccgcctc
     8641 ggcttcccgt ggaggctctg ctgaaggcgc cgcaaatccc gcttttcgcc gcgcctgatc
     8701 cttgcgccgt ttgagattgt ccttgagggc ggcggccaga cgggccggcc gttgattccc
     8761 cccttttcct ttattcgaca tctccgccat gaaggcgctt cttacccttt cgcggcggcg
     8821 caccttgggt tatagcagcg gtccttcggg cccaaacccg cttggatgcc gcagtagcgt
     8881 agtggtagcg caaccccttg gtaaggggta ggtcgcaagt tcaattcttg cctgcggcac
     8941 cagtttttcc gaaagacggc acgtttcgcc ggctcacgcg ttgtcccgcg cggcgccggt
     9001 gaaggcgcct gtcacggaga cgcccatgcc catcgtccat atcgatgtcc tgaccatcca
     9061 gccgctggtg gcgctgcttt tcggcatcct ggtcctgctg attccgcggc tgctgaacta
     9121 tctggtggcg atctatctca tcctgatcgg aatcatcggg ctttggcccc aattgttcac
     9181 ccacgtcacg tagccgtaag gattactggg cgttgtcgag ggccgccatg acggtggatt
     9241 cggtcagaag ctgcggcagt acggcgggtt tctccgcacc gggcgcataa tagagataga
     9301 gtggcacgcc cgagcgcccc tggctcgcga ggagcgcggt gatcgccgaa tcctgagttg
     9361 tccaatcggc cttcaaagcc gcgactttgc gcgccgcgaa ggcttctccc aacctgctgc
     9421 cggaaagcgc aaccctttca ttgacgagac aggtgatgca ccaggccgct gtggcgttga
     9481 tgaagacggg ccgtccctcc tgccgcaggc tctgaagacg gctcgcgctg aaaggctcgt
     9541 aagcgagccc gccgccttcc aggcccacgc ctgccgtgcc tgcgggccgc gcccccacgc
     9601 ccgtcagggg aatcagaacg atagccgcca ggatcgcaac cgccgccgcg gcaaacccca
     9661 gggtccggcc ggtgccatgc gccgcctggc tcctgccgta aatccacagc ccgaaggcga
     9721 gcgccagcgc gcctgccaac accgccagca ccccgtcgga acccgcctgc aaactcaaca
     9781 cccagacgag ccatatggcg gcgccataca tcggaaacgc cagcacctgg cgcagcgtcg
     9841 tcatccaggc gccaggacgc ggcagcacac cgagcgcgcg gggccacaag ccgagcacca
     9901 tgaagggcgc ggcgaatccg acccccagac ccgcgaatat ggcgagcgca acgcccgcat
     9961 tttgggtcaa cgcatagccc atggccgcgc ccatgaaggg cgccgtgcac ggggttgcca
    10021 caaccaccgc caacacaccg gtgaagaagc tgcccgccgc tccctccttg cccgccagcc
    10081 ggccgcccat gccggaaagc cttccgccgc cgatttcata aagccctgac aggttgagac
    10141 cgaccgcgaa catcaggagc gcaagcgcgc tcacgaacag cggctgctgc aattgaaagc
    10201 cccagccgag gcccgcgctg ccgccgcgca agacgaccag cgcgccgcca agcgcaagaa
    10261 agctcacgag cacgcccgcg ccataggcaa gcgcggccgc cttggcacgg tgcggggctt
    10321 cgcgtttggc cgcgagcgcc aacgccttca tcgacagaac gggaaagaca cagggcatca
    10381 gattaaggat gagcccgccc aggaccgcga acagaagcgc cgccgcgaac ccgacctcgt
    10441 tggaagtgtt ctggagcagc gtcacggacg acgccggcac cgggccgggc tcggcgtcca
    10501 catccagcgc atcgacacgg ccgtccgcgc cggtcagcac caacacaccg ccgatcttcc
    10561 cggcactggc acgtttttct tgcgtggcga gtttgcggtc ggtgaccccc tcgatcacga
    10621 aacccttgtc gctggtcccg agacgctgcg gcgcgccggc ctgcacgtat ccatccgtgt
    10681 acggatagaa cgccacatcg cgcgggcggg cgctgaccag ttcgggactt tcgatcagaa
    10741 gcgcgaaacg cttgtcgccg gcatcgtaga cggccttcca gggtgaggca tgggcaagct
    10801 tggaacgcgc gtccgcgaag aacgcggccg tcttcccatc gggcggaggt gtttgcgccg
    10861 caatgccgag cgggacgctg acatgacttt cttctggcac gcagacaccc gacgcatccg
    10921 tgcagacgag atacatcagg tccgccgaca cgatggcgcc ctcgcccggc ttcgcgtcag
    10981 ccggcaccga aatgtcgacc ggcagggaga cttcgtcctg gtagccatac gtcatcaact
    11041 gttcgagcgg caggcgcttg ggatagggcc attggatgtc ccccgccttc cagccttccg
    11101 gaagctgcca tttcgcggtg gtgggaaaac cggcgtcgcc cgggttgcgc caataggtat
    11161 gccagccttt gcggatag
//
LOCUS       DASYEG010000328         2980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2304027, whole genome shotgun sequence.
ACCESSION   DASYEG010000328 DASYEG010000000
VERSION     DASYEG010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2304027"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VGM72_13850"
     CDS             <1..904
                     /locus_tag="VGM72_13850"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY4266396.1"
                     /translation="IVGFAPGGGVDTTTRVVAKHLARFIPGAPRIIVQNMEGAAGVVS
                     CNYLYQRVAADGLTIGVPGRSWFVEGAIRVADVRFDAEKFSYVGSPGAVNSVVYVRTS
                     TGVRSFSDLKAAKRPLTFGALGNLTPTAMVPMMLAANGLPVKVVVGYVSSARVIVALE
                     QGEVDGFFTVEASFGLRQDLVSKHIIVPILQNKPVHPGLPLIRDALPQSDGALLRVVM
                     ALETFGLPVIGPPAIPAEWLEILRSAFLAMCRDKDFQADAVKADLPVGNPIGGAQLAT
                     MMTELVATATPALIARYKRLGTPV"
     gene            954..1337
                     /locus_tag="VGM72_13855"
     CDS             954..1337
                     /locus_tag="VGM72_13855"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HEY4266397.1"
                     /translation="MIKSAIPAVMLALAGTIAPVAAHHSDAMFATDKEVVLMGTVKEF
                     QYTNPHSWIQLVVPASNGPAVEWSIETGAPIVLLRAGIKPTALHPGDKITLRAHPLKD
                     GAAGGSLIEVTKEDGSKLTLKGPAY"
     gene            1354..2235
                     /locus_tag="VGM72_13860"
     CDS             1354..2235
                     /locus_tag="VGM72_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266398.1"
                     /translation="MTRHDLPNRRNVGAGLIAAGTLAVAAPLRAAEQSHDETVRKFAA
                     LPDWTGIWTGTGTLFEQRGGRGTPNGNPDARDFPPYKPEWEAAYVKFLNDVVKPGKFS
                     DPLSLGYPGGMIRMMSPARGLQFVLRPEQVWIIYERPDVRYIYTDGRPFPPKDELWPT
                     FEGYSIGHWEGDTLVIETRSIRGGIPLDRTGACLSDEAQVLERIRKADDHTLQSDITI
                     EDPVALTGPWKVTRRYTKRREEYPRMENVSSLENQRNPVVNGETKILLAGDSDASSPY
                     PDEIRPFAIPKFPFPHK"
     gene            2357..>2980
                     /locus_tag="VGM72_13865"
     CDS             2357..>2980
                     /locus_tag="VGM72_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266399.1"
                     /translation="MMTALEQLKLLRDPFGRESPPPIGQPVQTAFDALVSRIDAGEAV
                     LVLNGPAGSGKTFLLDMIDRFYSGRGSCVRRVTHGDLAHMALGARCDLLLVDEADSLD
                     QATIQGLLSNAGAARPSSVLFACVSPVIAASIAKDDSSLLELTLFAPADARSFLLERT
                     GGAGRPDLLEPEALDALIAGSGGSPQLLQSIGGHALFLASYDRSARIA"
BASE COUNT          527 a          932 c          931 g          590 t
ORIGIN      
        1 gatcgtcggt ttcgcgccgg gcggcggcgt ggacaccacc acgcgcgtgg tggccaagca
       61 tctggcgcgc ttcattcccg gggccccgcg catcatcgtg cagaatatgg agggcgctgc
      121 gggcgtggtc tcctgcaatt atctgtacca gcgcgtcgct gccgacggat tgaccatcgg
      181 cgtgccgggc cgctcctggt tcgtcgaagg cgcgatccgt gtcgccgacg tgcgcttcga
      241 cgcggagaaa ttttcttatg tcggcagtcc gggcgcggtg aattcggtgg tctatgtccg
      301 caccagcacc ggcgtgagaa gcttttccga tctgaaagcc gccaaacgtc cgctgacctt
      361 cggtgcgctc ggcaatctga cgcccaccgc gatggtgccc atgatgctcg cggccaacgg
      421 actgccggtc aaggtggtgg tcggctatgt ctcgagcgcg cgcgtcatcg tggcgctcga
      481 acagggtgag gtggacggct tcttcaccgt ggaggcatcc ttcggcctgc ggcaggactt
      541 ggtgtcgaaa cacatcatcg tgccgatcct gcagaacaag ccggtgcatc cgggattgcc
      601 gctcatccgc gatgcgctgc cgcaaagcga cggggcgttg ctgcgcgtgg tgatggcgct
      661 cgaaaccttc ggcctgccag tgatcggtcc gcctgcaatt cccgccgaat ggctggagat
      721 cttgcgcagc gcctttcttg ccatgtgccg cgacaaggat ttccaggccg atgcggtgaa
      781 ggcggatttg ccggtcggca atccgattgg cggcgctcaa ctcgccacga tgatgacgga
      841 gttggttgcg accgcgaccc cggccctcat cgcccgctac aagcgtctgg gcacgccggt
      901 atgaattgct ctattcttga cggcctattg gaccgcctat tggagttacg cccttgatca
      961 aatccgcaat cccggccgtc atgctggcgc tcgcaggaac tatcgcacct gtcgcggcgc
     1021 atcattccga cgccatgttc gcaaccgaca aggaggttgt gctgatgggg acggtgaagg
     1081 aattccaata caccaacccc cattcctgga ttcagctggt ggtgccggcg tccaatggtc
     1141 cggcggtcga atggagcatc gagaccggcg cgcccatcgt gctcctgcgg gccggcatca
     1201 agcccaccgc gctacacccg ggcgacaaga tcacgctgcg tgcccatccg ctcaaggacg
     1261 gcgccgctgg cggctcgctg atcgaggtaa cgaaagagga cggttccaag ctgacgctga
     1321 aaggcccagc ctattgatgt gcggggtgtt cccatgacca ggcatgatct tcccaatcgc
     1381 cgcaatgtgg gtgccgggct catcgccgcg ggtaccctcg cggtcgcggc cccactgcgc
     1441 gctgcggaac aatcccacga cgagaccgtg aggaaattcg cagcactccc cgattggacc
     1501 ggcatctgga ccgggaccgg caccttgttc gagcagcggg gcggcagggg cacgcccaac
     1561 ggcaatccgg acgcgcgcga ttttccgccc tacaaaccgg agtgggaagc ggcttacgtc
     1621 aaattcctga acgatgtcgt gaagcccgga aaattctccg atcccttatc gctgggctat
     1681 ccgggcggga tgatccgcat gatgtcgccg gcccgcggcc tgcaattcgt gctgcgcccg
     1741 gagcaggtct ggatcatcta tgaacgtccc gacgtgcgct acatctacac cgatgggcgt
     1801 ccctttccgc ccaaggatga attgtggccg accttcgagg gttattccat tggacactgg
     1861 gaaggcgaca cattggtcat cgaaacccgc agcatccgcg gcgggattcc gctcgaccgt
     1921 accggcgcat gcctgagcga tgaggcccaa gtcctcgagc gcatccgcaa ggccgacgat
     1981 catacgctgc aatcggacat cacgatcgaa gaccctgtcg cgttgaccgg tccatggaag
     2041 gtgacgcgcc gctataccaa acgccgcgag gagtatccgc ggatggagaa tgtttcgagc
     2101 ctggagaacc agcgcaatcc cgtggtcaat ggcgagacca agattcttct ggcgggggat
     2161 tccgatgcat cctcacccta tcccgacgag atcagaccat tcgccattcc gaaatttcct
     2221 tttccgcaca aatgataagc cgttgtggca tttttgtgat cttcattgcc atgggcgcgt
     2281 ctaagctaat gtgccgcccc atttgttaac catgggggtc gtgaactgac ctatgctccc
     2341 gttccaagga gaggccatga tgacggcttt ggaacagttg aagctgttgc gcgacccgtt
     2401 cggcagggag tctccgccgc cgataggaca accggtgcag acggcgttcg acgcgctcgt
     2461 gagcagaatt gatgcgggag aggctgtcct cgtcctgaac ggtccggccg gaagcggaaa
     2521 gacgtttctc ctggatatga tcgaccgctt ttattcgggg cgtggaagct gcgtgcgtcg
     2581 cgtgacgcat ggcgatcttg cccatatggc ccttggcgca cgctgcgacc tgctgctggt
     2641 ggatgaggcc gattcgctgg atcaggcgac gatccaaggc ttgctctcca atgccggcgc
     2701 cgcccggcct tcgagcgtcc tgtttgcctg cgtttctccg gtcatcgccg cttccattgc
     2761 caaggacgat tcaagcttgc tcgagctcac gctattcgcg cctgcggacg caaggagttt
     2821 tctgcttgaa cggacgggcg gcgccgggcg gcccgacctc ctcgagcccg aggcgcttga
     2881 cgccctgatc gcgggcagcg gcggttcacc gcagctcttg caatcgatag gcggacacgc
     2941 gcttttcctg gcgtcctacg accggtcggc ccgcatcgcg
//
LOCUS       DASYEG010000329         5688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2449426, whole genome shotgun sequence.
ACCESSION   DASYEG010000329 DASYEG010000000
VERSION     DASYEG010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2449426"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VGM72_13870"
     CDS             complement(<1..199)
                     /locus_tag="VGM72_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266400.1"
                     /translation="MVIRIGATVLNNDENSFARADNLKAKAARYRWLADILFDPKIAA
                     VVQACARELDCEAKFLEQSENR"
     gene            complement(333..794)
                     /locus_tag="VGM72_13875"
     CDS             complement(333..794)
                     /locus_tag="VGM72_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266401.1"
                     /translation="MKIDFREDGPMSNRRAMLHAVLGVFALILVANNARAQTCFVHPA
                     RIIDSLAGLPTLIRGDLSAMSDKDGPFNGSDTIVPGVPITRFISAAQIGDVYFVWYEL
                     GGIFYSKALALYRMKAENSVSERLAQHVYALEPEAVCKIVDDVLDGKAPPG"
     gene            complement(876..2405)
                     /locus_tag="VGM72_13880"
     CDS             complement(876..2405)
                     /locus_tag="VGM72_13880"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY4266402.1"
                     /translation="MRTKMPFVIVPLLAIWAAGASHAQITSNPIPTPIEKKGLAVEIK
                     DVVRLPDTRGIHGADDVTPSGWARISYVRDLPDGRRFVNDSRGLLYLLDSNNQPHVYA
                     DVAATFPYGVYNRLESGFIGFVFHPEFAKNGLFYTVHSERGPGNPKTPDFIPPGYTLK
                     DVTYHNVITEWHATDPAANVFAGTRREILRESHVVANLTHPMGAVEFNPTAKPGDADY
                     GLLYTSGSDHGFSNGGGPNASNPAQTQRLDSIITAILRIDPRSPSVTHGVKGLGDYTI
                     PMANKFAADGDPKTLGEIYAYGFRNAHRLSWDMADGTMFASDIGMDNIEEINIVRNGE
                     NYGWMKREGYFENGRSRGRALNELFPLPPEILDGRQKDGFTYPVAVYDHDEGRSVTDG
                     FAYHGRIAALRGKFVFGDIQTGRVFASDLAAMKKADDGIPQTVAPIEEVQLYVRDASG
                     KRVDVSMRDLIQKTMGKSLPRADLHISRSGDGELFLTSRQDGMIRTLVPDESGRAAAA
                     R"
     gene            2559..3320
                     /locus_tag="VGM72_13885"
     CDS             2559..3320
                     /locus_tag="VGM72_13885"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY4266403.1"
                     /translation="MSAARLIMQGLAVCAALCVFGGSADASEIKLLASNAVKEAYGLL
                     LPAFEKASGNKVTVVWGGTADIIRRIEGGEPADIVIITDAGIDRLIGAGKLTAMGRVT
                     VAKSGVGAAVRAGGSKPDLSSGDALKKSLLAAKAIVLTPGPSNPLLHQLFDRMGLAKE
                     MKAKEIRPMPGQQLTDPVIAGKADIVFSQTSELIAVKGIEYAGPLPSGSQIVLTYDAA
                     FHAGAPQPGAAKDLVKFLTTPAAARMLQQNGLEPG"
     gene            complement(3327..4511)
                     /locus_tag="VGM72_13890"
     CDS             complement(3327..4511)
                     /locus_tag="VGM72_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY4266404.1"
                     /translation="MDRRDVVVVGAGGAGMMCAAQAATRGRSVLVLDHAPVPGEKIRI
                     SGGGRCNFTNLNCAPENFLSQNPRFCISALSRYSVGDFVALVEHYGIAYHEKTLGQLF
                     CDGSSGQIIDLLLSEMKRAGAELRLGTAVTNVEKTESGFLLTLSSGAEPVQCQSLVVA
                     TGGRSIPKMGASDFGYRLARRFDVGVTETRPALVPLVFEERTLARLKVLTGIGVTAHV
                     RCGETTFEEAILFTHRGLSGPAILQISSYWREGEDIVIALLPSTDLFETLRDARKTNG
                     RQQLSTCLSEFLPKRLAQMIVEEDGVAGRLGDLSDKVLHRIDEAVNFWRVRPSGSEGY
                     RTAEVTLGGVDTNDLDSRTMAVKRVPGLYFIGEVVDVTGWLGGYNFQWAWSSGWCAGQ
                     AV"
     gene            4600..4851
                     /locus_tag="VGM72_13895"
     CDS             4600..4851
                     /locus_tag="VGM72_13895"
                     /inference="COORDINATES: protein motif:HMM:NF023158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3297 family protein"
                     /protein_id="HEY4266405.1"
                     /translation="MTDTPPDRLATNPKNPHHNAALLERGVGIRFNGVEKTNVDEYCV
                     SEGWIRVTVGTTVNRAGQPLTIKLNGKVEPYFRDDPEKR"
     gene            5049..>5688
                     /locus_tag="VGM72_13900"
     CDS             5049..>5688
                     /locus_tag="VGM72_13900"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4266406.1"
                     /translation="MARIIHIALKVEDLESATKFYEDVFGFRQTGTGHARGHISRHMT
                     DGELDVALMVYDSEEEHEAQLSGAGPCIHHWGIEVDDRDATVKLIEANGGAIISEPGQ
                     GALKFRAPDGTVAEIVKAGRYKKNAQAGGSRIVHLAIKVTDLESATRFYENVFGFRQV
                     HTGRNRQHISRHMTDGAFDLALMVYDSEDAHEAQLAGPGPRIHHWGIEVDDRE"
BASE COUNT         1161 a         1725 c         1691 g         1111 t
ORIGIN      
        1 ggcgattctc ggactgttcc aggaatttcg cctcacagtc caactcccgc gcgcacgctt
       61 gcacgacggc ggcgatcttc gggtcgaaca gaatatcggc aagccatctg taacgcgcgg
      121 cttttgcctt cagattgtct gcacgggcga aactgttttc gtcgttgttc aacactgtgg
      181 cacctatgcg aatcaccatc cacagcattg ctcgtacgag ttaatcaggt cttaaaagat
      241 gtcccattgc ggcacagcgg gcttccgatt gctgtgataa gcatgaggcc tacggccacg
      301 tctcagtctc gcgcgcagct tgcgtgcgcc ggtcagcccg ggggcgcttt tccatccaga
      361 acgtcgtcca cgatcttgca aacggcttcc ggttccagcg cataaacgtg ctgtgccagg
      421 cgttcggata cgctgttctc tgccttcatg cgatagagcg cgagcgcctt ggaatagaag
      481 atcccaccca attcgtacca aacgaaatag acatcgccga tctgtgcggc actgataaag
      541 cgcgttatcg gaacgccggg aacgatagtg tccgaaccgt tgaacggccc gtccttgtcc
      601 gacatcgcac tcaagtcgcc gcgaatgagt gtgggaaggc cagccaggga atcgatgatg
      661 cgcgccgggt gaacgaaaca ggtctgcgcg cgggcgttgt tcgccaccaa tatcagtgca
      721 aaaactccca gcacggcatg gagcatcgcc cggcggttcg acatcggtcc atcctcgcga
      781 aaatcgatct tcatagacca tccgaccccg ccatctggac acagaaatgg ccaatttcgc
      841 aacaccgaaa agtgaaattg aaacgatgtt cgcactcacc gtgccgccgc tgcgcgaccg
      901 ctttcgtccg gaacgagcgt tcggatcatg ccatcttgcc gcgaggtcag gaaaagttcc
      961 ccgtcgccgc tgcggctgat gtgcaggtca gcgcgcggca ggctcttgcc catcgtcttc
     1021 tggatcaggt cgcgcatcga cacgtccaca cgtttgccgc tggcgtcgcg gacatagagt
     1081 tggacctctt cgatcggcgc caccgtctgc gggatgccgt catcggcctt tttcattgcg
     1141 gcaaggtcgg atgcgaacac gcgccctgtt tggatatcgc cgaacacgaa cttgccgcgt
     1201 aaagccgcga tacggccgtg atacgcgaac ccatcggtta cagaccggcc ctcatcgtga
     1261 tcgtagaccg caacgggata ggtgaagcca tccttctgcc gtccatccag gatttcgggc
     1321 ggcagcggaa acagctcgtt gagcgcacgc cctcgtgacc ggccgttttc gaaatagcct
     1381 tcgcgcttca tccagccata attttcgccg ttccggacga tgttgatctc ctcgatattg
     1441 tccatgccga tgtcggaggc gaacatcgtc ccgtcggcca tgtcccatga cagccggtgg
     1501 gcgttgcgga atccataggc atagatttct ccgagcgttt tgggatcgcc atccgcggcg
     1561 aatttgttcg ccatcggaat ggtgtaatcg ccaaggccct tcacgccatg cgtcaccgat
     1621 gggctacgcg gatcgatgcg caggatcgcg gtgatgatcg agtccagccg ttgcgtctgc
     1681 gccggattgc tggcgttcgg tccgccgcca ttgctgaatc catgatcgct gccgctggtg
     1741 tagagcaggc cgtaatcggc atcgccgggt ttcgccgtcg ggttgaattc cactgcgccc
     1801 atcgggtggg tgagattggc gacgacgtga gattcccgca ggatctcgcg ccgcgtgccc
     1861 gcgaaaacat tcgccgccgg gtccgtcgcg tgccactcgg tgatgacgtt gtggtaggtc
     1921 acgtctttca acgtgtagcc gggcgggatg aagtcgggcg tcttgggatt gccgggcccg
     1981 cgctcgctgt gcacggtgta gaacaggccg ttcttggcga attcgggatg gaagacgaag
     2041 ccgatgaagc cgctctccag ccggttgtag actccatacg gaaacgtggc cgcgacatcg
     2101 gcatagacgt gcggctgatt gttcgaatcg agcagataca acaagcctcg cgaatcattg
     2161 acgaagcggc gtccatctgg aaggtcgcgc acatagctga tccgcgccca gcccgatggc
     2221 gtcacgtcgt cggcgccgtg aatcccccgc gtatcgggca gacgcacgac atccttgatc
     2281 tcgaccgcga gacccttttt ctcaatgggg gtcggaatcg gattgcttgt gatctgcgca
     2341 tggctcgcgc ccgcggccca gattgccaga agcgggacaa tgacgaatgg catctttgtg
     2401 cgcatgaagc cctcccattt ctttgtctgt agagcatgct tataccagat cgccgggcgc
     2461 tcgccacgcc gtgcggcgca agcacaatgg ggccggggcg ccattgttcg cgcgcgatat
     2521 ttgatgcaat gatcgtttgc ttcgggaagg atcatacgat gtctgcggca cgtctcatca
     2581 tgcaaggcct cgcggtctgt gcagccctct gcgtgttcgg cggttcggcc gatgcgtccg
     2641 aaatcaaatt gctcgcctcc aatgcggtca aggaggccta tggccttctg cttcccgcgt
     2701 tcgaaaaggc gagtggaaac aaagtgacgg ttgtctgggg tggaaccgcc gacatcatca
     2761 ggcgcatcga aggcggggag cccgccgaca ttgtcatcat caccgatgcg gggatcgatc
     2821 gcctgatcgg cgcaggaaaa ttgaccgcaa tggggcgtgt cacggttgcg aaatccggcg
     2881 tcggtgccgc ggtgcgcgcc ggcgggtcca aacccgactt gtcttcaggt gacgcgctca
     2941 aaaaatcctt gctcgcagcg aaggcgattg tgctgacacc cggtccgagc aatcctcttc
     3001 tgcaccaatt gttcgaccgg atgggacttg caaaggaaat gaaagcaaag gaaatccggc
     3061 ccatgccagg acagcaattg acggacccgg tcattgcagg caaggcggac atcgtgtttt
     3121 cgcagacgag cgaattgata gccgtgaaag gcatcgaata tgccggaccg ttgccgtccg
     3181 gctcgcaaat cgttctgacc tacgatgccg cgttccacgc cggggcgccg cagccgggcg
     3241 ccgccaagga cctcgtgaaa ttcctcacca cgcctgccgc tgccaggatg ctgcaacaga
     3301 acggcctcga gcccggctga ggcaaatcag accgcctgcc ccgcgcacca ccccgacgac
     3361 caagcccatt ggaaattgta gccaccgagc cagcccgtca cgtccaccac ctcgccgatg
     3421 aaatagagtc ccggcacgcg cttgaccgcc atggtccggg agtcgaggtc attggtatcg
     3481 acaccgccga gcgtgacttc ggcggtgcga tagccttcgc tgcccgaagg ccgcacacgc
     3541 cagaaattca ccgcttcgtc gatgcggtgc aggaccttgt ccgaaagatc gcccaatcgg
     3601 cccgccacgc cgtcttcctc gacaatcatc tgcgccagcc gtttgggcag aaattccgaa
     3661 aggcaggtgg aaagctgttg gcgtccattg gttttgcgcg cgtcgcgcaa tgtttcgaaa
     3721 agatcggtcg aaggcagcag cgcgatgacg atgtcctcgc cctcgcgcca ataggatgaa
     3781 atctggagga tcgccggtcc gctcaaaccg cgatgggtga acagaatcgc ttcctcgaat
     3841 gtcgtttcgc cacagcgcac atgggctgtg accccgatcc ccgtcagcac tttcagccgc
     3901 gccaacgtgc gctcctcgaa gacaagcggc acaagggccg gccgtgtttc agtgacgcca
     3961 acatcgaagc gccgcgccag cctgtagccg aaatcgctgg cgcccatctt gggaatggat
     4021 ctgccgcccg tcgccaccac cagcgattgg cattgcaccg gctccgcgcc cgaggacagg
     4081 gtcagcagaa agcccgattc ggttttttcg acgtttgtca ccgccgtccc caggcggagc
     4141 tccgcgcccg cccgcttcat ctccgacagc agcaggtcga tgatctgtcc cgacgaaccg
     4201 tcgcagaaaa gctggccgag tgtcttttcg tgataggcga tgccgtaatg ttccaccaaa
     4261 gccacgaaat cgcccacgct atagcgactg agcgcggaaa tgcagaaacg cggattctgc
     4321 gacagaaaat tttcgggggc gcagttgaga ttggtgaaat tgcatcgtcc gccgccggag
     4381 atgcggattt tctctcccgg aacaggagca tgatcgagga cgagaacgga gcgcccgcgc
     4441 gtggccgcct gtgccgcgca catcatgccc gctccgccgg cgccgacaac aaccacgtcc
     4501 cgccgatcca ccagtgtctc ctaaacgatt taaatcctcg ccgggaacgg ctatcacatt
     4561 cctgcgtctg atgttggaac gtccctgaaa gctatcgcca tgaccgacac acctcccgat
     4621 cgcctcgcca cgaatcccaa aaatccccat cacaatgccg cgctcctcga aaggggcgtg
     4681 ggaatccgct ttaacggagt cgagaagacc aacgtggacg aatactgcgt gagcgaaggg
     4741 tggattcgcg tgacggtcgg taccaccgtg aaccgggccg gtcagccatt gaccatcaag
     4801 ctcaacggga aggtcgagcc ctatttccgt gacgatccgg agaaacgcta gtagccgcca
     4861 ggcaggcttt ttccgcatca tggcggtttc ctcctggccg gcgatccgac ggatttgtaa
     4921 cgcggcgacg gaaaacgagg ttactgcatc gggttttccc gatccggagg taagggcgct
     4981 tgtggaggtg gctttcgccc cataaagtcc tggcgttttc gcatctttcg caaagaaacg
     5041 gcagggtcat ggcgcgcatt attcatatcg ctctcaaggt cgaggatctc gagagcgcca
     5101 caaaattcta tgaagacgtg ttcggatttc gccagaccgg caccggccat gcgcgcggtc
     5161 acatatcgcg tcatatgacc gacggagagc tcgatgtcgc gctgatggtt tacgacagtg
     5221 aagaggaaca tgaggcgcag ctatcgggcg ccggtccctg catccatcat tggggcatcg
     5281 aggtggacga tcgcgacgcg acggtcaaac tgatcgaggc gaatggcggt gccattatct
     5341 cggagcccgg ccagggcgcg ctgaaattcc gggcgccgga cggaacagtg gccgagatcg
     5401 tgaaggccgg acggtacaag aagaacgcgc aagcgggcgg ctcacgcatt gtgcatctgg
     5461 cgatcaaggt cacggatctg gaaagcgcca cgcgcttcta tgaaaacgtc ttcgggttca
     5521 gacaggtgca cacgggccgc aatcgtcagc acatctcgcg gcatatgacg gatggcgcgt
     5581 tcgatcttgc gctcatggtc tatgacagcg aagacgcgca tgaggcacag cttgccggcc
     5641 cgggaccgag gatccatcat tggggcatcg aggtcgacga ccgtgagg
//
LOCUS       DASYEG010000330         2320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2740838, whole genome shotgun sequence.
ACCESSION   DASYEG010000330 DASYEG010000000
VERSION     DASYEG010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2740838"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2292
                     /locus_tag="VGM72_13905"
     CDS             <1..2292
                     /locus_tag="VGM72_13905"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY4266407.1"
                     /translation="CTVEAMPLEIAVACLVVDEIQLCADPERGHVFTDRLLHARGTEE
                     TLFLGAESMRGAIQRFIPRTWFITRPRFSDLAYTGAKKLTRLPRRSAVVGFSAEDVYG
                     IAEIVRRQRGGAAVVLGALSPRTRNAQVALYQSGDVDFLVATDAIGMGLNMDVDHVAF
                     AGSEKFDGRGFRPLAASEIGQIAGRAGRYMNDGTFGVTGTCESFDEEAVARVESHRYD
                     PVKVLQWRNADLQFQSVAALLESLDAPPPERGLGRARPASDAIALHHLASDPDVAALA
                     TSRSAVQRLWEVCQLPDFRKLSADEHARMIRTLYLFRMGEPGYVPDDWMARQIARLDV
                     VEGDVATLSGRLAQIRTFTYAAHRPGWTRDGAHWQNETRAVEDRLSDALHEQLTLRFI
                     DRRASVLMKHLREDENGEVVLEEDGGVLIAGEVIGRLDGFRFTPDPRTAGEGNGVLHV
                     RMARTAAMRGLESEFLARARSLEAAPDAAISLSEHGKLWWDGAMVGHLAAGASPLAPQ
                     IVLAADDNLKGVARDNVQTRLETWVRTRIDTRLEPLVALARAAEARAGSDTALPAYAR
                     GLAHQLAENFGALERDALALPEKLGPVLRALKPFGVWFGRRTVYLPRLLRPDAAGLLA
                     LLWGIWTRQAQLAGPPAPGLTSFAYSGEAKASLHAAGFAVIGTRAIRFDMLERLEETL
                     DKATINGADAASLLPQLVSLMGTGNDEAKAVLAALGWRLTEVADARPVWRRVKKKEPR
                     KRAPEKPPPENSPFAGLKELLVK"
BASE COUNT          396 a          748 c          752 g          424 t
ORIGIN      
        1 tgcaccgtcg aagccatgcc gctggaaatt gccgtcgcct gtctggtggt ggacgaaatc
       61 caactttgcg cggacccgga gcgcggccat gtcttcaccg accggctgct tcatgcccgg
      121 ggtacggaag aaacattgtt cttgggcgcc gaaagcatgc gcggtgccat tcaacgcttc
      181 attccgcgca cctggttcat cacccgaccc cgcttttccg atcttgccta taccggggcg
      241 aagaaactca cccgcctgcc gcggcgttcg gccgtggtcg gcttcagcgc cgaggatgtc
      301 tatggcatcg ccgaaatcgt gcggcgccag cgcggcggcg cggctgtcgt cttaggggcg
      361 ctttcaccac gcacccgcaa cgcccaggtc gcgctgtatc aatccggcga tgtcgatttt
      421 ctggtcgcca ccgatgcgat cggtatgggc ctcaacatgg acgtcgatca tgtcgccttt
      481 gcgggatcgg agaaattcga cggccgcggc tttcgccccc tcgccgcatc ggagatcggt
      541 cagatcgcag ggcgcgccgg acgctacatg aatgacggca cgttcggcgt taccggcacg
      601 tgcgagtctt tcgacgaaga agccgtcgcc cgcgtcgaaa gccaccgcta tgacccggtg
      661 aaggtcctgc aatggcgaaa tgccgatctg caatttcaat ccgttgccgc gctgctggaa
      721 agtctggacg cgccgccgcc cgaacgcgga ctgggccggg cgcggcccgc ctccgatgcg
      781 atcgcgcttc atcatctggc cagcgatccc gacgtcgccg cactcgccac atcgcgaagc
      841 gcggtccaac gcctttggga ggtctgccag ttgccggact tccgcaaatt gtccgcggac
      901 gaacatgcgc gcatgatacg cacgctctac ctcttccgca tgggcgagcc cggatatgtc
      961 cccgacgatt ggatggcgcg gcaaatcgcg cggctcgatg tcgtggaagg ggatgtcgct
     1021 acgctgtcgg gacgtcttgc ccagatccgc accttcacct atgccgcgca ccggcccggc
     1081 tggacgcgtg atggcgcgca ttggcaaaac gaaactcgcg ccgtcgaaga ccggctttcc
     1141 gatgcgctgc acgaacagct taccttgcgc ttcatcgacc ggcgcgcatc cgtgctgatg
     1201 aaacatctgc gcgaagacga aaatggcgaa gtcgtgctgg aggaagatgg cggcgtcctg
     1261 atcgcaggcg aagtgatcgg ccgcctcgac ggattccggt tcacgcctga tccgcgcacc
     1321 gccggcgaag gcaatggcgt gcttcatgtc cgcatggccc gcacggcggc gatgcgtggg
     1381 ctggaaagcg agtttctggc gcgcgcccgc tctctggaag ccgcgcccga tgccgcgatc
     1441 agtttgagcg agcatggcaa gctgtggtgg gacggcgcga tggttggcca ccttgccgcc
     1501 ggcgcttcgc cgcttgcacc gcagatcgtg ctggcggcgg atgacaatct caaaggcgta
     1561 gcccgcgaca atgttcaaac gcggctcgaa acttgggtgc gcacgcgcat cgatacgcgg
     1621 ctggagccgc tggtcgcgtt ggcgcgggcg gccgaggcgc gggccggcag cgataccgcc
     1681 ctacccgcct atgcgcgggg cttggcgcat cagttggcgg aaaatttcgg tgcgctggag
     1741 cgcgatgctt tggcgttacc ggaaaagctc ggtcccgtct tgcgtgcact caaaccgttc
     1801 ggcgtctggt tcgggcggcg cacggtttat ctgccgcgcc ttctgcgtcc cgatgcggcg
     1861 ggattgttgg ccttgttgtg gggcatctgg acacgccaag cgcaactcgc cggaccgccc
     1921 gcgccgggcc tgacatcttt cgcctattcc ggcgaagcaa aagcgtctct gcacgccgcc
     1981 ggttttgcgg tgatcggcac gcgcgccatt cgtttcgata tgctggagcg gctggaagag
     2041 acgctggaca aagccacaat caatggcgcc gacgcggcca gtctgttgcc gcagctcgtc
     2101 tcgctcatgg gaaccggcaa tgacgaggcc aaggcggttc tcgcggcgct gggatggcgg
     2161 ctgaccgaag ttgccgacgc gcgcccggtc tggcgccgtg tgaaaaagaa agaaccacgc
     2221 aaacgcgcgc ccgaaaagcc gccgcctgaa aactcgccct ttgccggtct gaaggaactg
     2281 ctcgtcaagt gaacgcgccg gatgatcctc tcgtgagcac
//
LOCUS       DASYEG010000331         2785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2742537, whole genome shotgun sequence.
ACCESSION   DASYEG010000331 DASYEG010000000
VERSION     DASYEG010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2742537"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VGM72_13910"
     CDS             complement(<1..315)
                     /locus_tag="VGM72_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY4266408.1"
                     /translation="MKSTHAIAALSALSQETRLSVFRHLVAAGPQGRPAGLIAARLRI
                     PAPTLSFHLKELERAGLITRHRESRSIIYAANYAAMRALLSYLMEDCCGGRPEICNIE
                     VKC"
     gene            complement(356..1141)
                     /gene="fliP"
                     /locus_tag="VGM72_13915"
     CDS             complement(356..1141)
                     /gene="fliP"
                     /locus_tag="VGM72_13915"
                     /inference="COORDINATES: protein motif:HMM:NF009438.0"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEY4266409.1"
                     /translation="MVWLPRALAIGACALVPSVAAAAQAAGGAAGAAAGQGLTLDLTG
                     GGAMSERLMQIVGLITILSLAPSILIMMTCFTRIVVVLSLLRTAIGVQQSPPNSVIVS
                     LALFLTFFVMSPTFTDIYQNAVQPLESQQITTDEALTRASTPIKKFMGAHTRNEDLSL
                     FVNMAGVSAPASAEGAPLQAIVPAFMLSELKRAFEIGFLIFVPFLIIDMAVASILMSM
                     GMMMLPPAVISLPFKLIFFVLVDGWRLVAGSLVASYGVGIAPQ"
     gene            complement(1159..1434)
                     /locus_tag="VGM72_13920"
     CDS             complement(1159..1434)
                     /locus_tag="VGM72_13920"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEY4266410.1"
                     /translation="MDMTDALRYFGALALVLALVGVAGLAIRRYGLPGIAGGTGRRLA
                     VVETLMLGKNHRLFIVRCDGTEHVLVTAPQGASVIATGPAKRADIAV"
     gene            1642..2052
                     /gene="flgB"
                     /locus_tag="VGM72_13925"
     CDS             1642..2052
                     /gene="flgB"
                     /locus_tag="VGM72_13925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEY4266411.1"
                     /translation="MNLNGVPLFSLLSDRMSWLSTRQSVLAQNVANADTPNYVARDIK
                     PMDFESMVAGQNSGAGLTVTNAHHIDIRAQTGGNGFEEEDAEGEGGTPGGNVVSIEQE
                     MIKLSDTQIQYQTATNLYQKAVSMFRTALGGQSG"
     gene            2065..2475
                     /gene="flgC"
                     /locus_tag="VGM72_13930"
     CDS             2065..2475
                     /gene="flgC"
                     /locus_tag="VGM72_13930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEY4266412.1"
                     /translation="MDFTTSLYIAAAGLRAQSGRMRIIAENLANADSVSQSANGEPYR
                     RRIATMTTEFDRELNANVVKLGDPVPDNADFRLQYDPGNPAADKQGYTHLPNVNALVE
                     MMDMRDAQRTYEANLQVMDGARSMLTRTIDLLRR"
     gene            2487..>2785
                     /locus_tag="VGM72_13935"
     CDS             2487..>2785
                     /locus_tag="VGM72_13935"
                     /inference="COORDINATES: protein motif:HMM:NF014143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEY4266413.1"
                     /translation="MAVSFVAANAAYQAAAKAGGTGAAESAIQTGAAQGANGSFSDAL
                     ASAVKDAMGTMHTGEMAAAKGATGQGDVVQVVNAVTAAELTLQTVVAIRDRVISA"
BASE COUNT          608 a          859 c          838 g          480 t
ORIGIN      
        1 gcacttcact tcgatgttgc agatttctgg gcggccaccg cagcaatctt ccatcagata
       61 ggaaagcagc gcccgcatcg ccgcataatt ggccgcatag atgatgctgc ggctttcgcg
      121 atgccgggtg attagcccgg cgcgttccaa ttccttgaga tgaaaggaca gcgtcggcgc
      181 cggaatcctt aggcgcgcgg ctatcagccc cgcaggtctg ccttgagggc cggccgcaac
      241 cagatgacgg aataccgaaa ggcgggtttc ctgtgaaagg gcggagaggg cggcaatggc
      301 gtgagtagat ttcatagttt cataactatg aaattatcaa ctcttggtca agttcctatt
      361 gcggagcgat gccgacgccg tagctggcga ccagcgagcc agcgacgagc cgccagccat
      421 ccaccaagac gaagaagatc agcttgaaag gcagcgagat cacggccggc ggcagcatca
      481 tcatacccat ggacatgaga atcgatgcca ccgccatgtc gatgatgaga aacggtacga
      541 agatcagaaa tccgatttcg aacgcacgct tcaattccga aagcatgaaa gccggaacga
      601 tggcctgcag cggtgcgccc tcggcggagg ctggggcaga gacgcccgcc atattgacga
      661 atagcgaaag atcctcgttg cgcgtatgcg cgcccatgaa tttcttgatc ggtgtggagg
      721 cgcgcgtcag cgcttcatcc gtggtgatct gctggctctc cagaggctgc acggcattct
      781 gatagatgtc ggtgaatgtc ggcgacatca cgaagaaggt cagaaacagc gcgaggctca
      841 cgatcacgct gttgggcggg ctttgctgaa cgccgatggc cgtgcgcagc aaggacagca
      901 ccaccacaat gcgcgtgaaa cacgtcatca tgatgagaat ggaaggcgcg agcgaaagaa
      961 tggtgatcag acctacgatc tgcatcagcc gttcgctcat cgcgccgccg cccgtcagat
     1021 cgagcgtcag cccttgcccg gccgccgcac cggcagcacc acccgccgcc tgcgccgcgg
     1081 cggcaaccga cggcaccagc gcacaggcgc caatcgccag cgccctcggc agccaaacca
     1141 tgaaaatccg cttgctgttc atactgcgat gtccgcccgt tttgccggcc ccgtcgcgat
     1201 cacgctggcc ccttggggcg cggttaccag cacatgttcc gtgccgtcgc agcgaacgat
     1261 gaagaggcga tgatttttgc cgagcatcaa cgtctcgacg accgcaagcc gacgtcccgt
     1321 gccgccggcg attcccggaa gcccgtagcg cctgatggca aggcccgcga caccgacgag
     1381 cgccagcacg agcgcgagcg cgccgaaata gcgaagggcg tcggtcatat ccatgaagcg
     1441 agaacctctt gaccctgcaa ggcggcaaaa tccgcctatc tctgttcgca gggcgagggg
     1501 catcctcgtc gcgcatgctt aaccccacgt aaacgcaccc ggcaaaattt tctcggtgtt
     1561 cgttcccgga attaagccgc gattaagcat gagcgccgat gctgctcagg cagtacgcgc
     1621 ttttcctcaa tggaggcagc catgaacctg aacggcgttc ccctcttctc gcttctgtcg
     1681 gaccgcatgt cgtggctttc cacgcgtcaa tcggtgctgg cgcagaacgt cgccaatgcc
     1741 gacacgccca attacgtcgc gcgcgacatc aagccgatgg atttcgagag catggtcgcg
     1801 ggtcagaatt cgggggcggg ccttaccgtg accaacgccc atcatatcga catacgcgcg
     1861 caaaccggag gcaacggctt cgaagaagag gatgccgagg gcgagggcgg tacgcccggc
     1921 gggaacgtgg tgtccatcga gcaggagatg atcaagcttt ccgacacgca gattcaatat
     1981 cagaccgcga cgaaccttta tcagaaagcg gtcagcatgt tccgcaccgc gcttggcgga
     2041 caatcgggct aagaggatca agacatggac ttcacgacat cgctctatat tgccgccgcc
     2101 ggtcttcgtg cccaatcggg gcgcatgcgc atcatcgcgg agaacctcgc gaacgcggat
     2161 tctgtttcgc aatcggccaa tggcgagccc tatcgccgcc gcattgccac catgaccacc
     2221 gaattcgatc gcgaactgaa cgccaatgtc gtgaagctcg gcgatcccgt accggacaac
     2281 gccgatttcc gcctgcaata cgatcccggc aacccggccg cggacaagca aggctatacg
     2341 catctgccta atgtgaacgc gctcgtcgag atgatggaca tgcgcgacgc gcaacgcacc
     2401 tatgaagcca atctgcaagt gatggacggt gcgcgctcca tgctgacgcg cacgatcgat
     2461 ctgctgcgcc gctgagggat tgaatcatgg ccgtgagttt tgtcgccgcg aacgccgcct
     2521 atcaggccgc cgccaaggcc ggaggtaccg gcgccgccga aagcgccatc caaacaggcg
     2581 ccgcgcaggg agccaacggc agtttttccg atgcgctggc gtcggcggtg aaagatgcca
     2641 tgggcaccat gcacaccgga gagatggccg cggcaaaagg cgcgaccggg cagggcgacg
     2701 tggtccaggt tgtcaatgcg gtgaccgcgg ccgaactcac cttgcagacc gtggtggcga
     2761 tccgcgaccg cgtgatttcc gccta
//
LOCUS       DASYEG010000332         2419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3468467, whole genome shotgun sequence.
ACCESSION   DASYEG010000332 DASYEG010000000
VERSION     DASYEG010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3468467"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VGM72_13940"
     CDS             <1..739
                     /locus_tag="VGM72_13940"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY4266414.1"
                     /translation="LRSTLALLKPHLSAKKPLVLCAKGIEHDTHRLMTEVLVECIPDC
                     EPAILSGPSFAKDVARNLPTAVTIAAKLEIARALQEAFGHLTFRPYASDDLTGVALGG
                     AAKNVYAIACGIVAGMGLGESARAALLSRSFAELCRLGEAMGARSETLMGLSGLGDLV
                     LTATSASSRNYAFGLDVGKGLSREELYKPGRPLAEGVATAPALAARAHEHKVELPIAE
                     TTSEILQGKIALADAIPRLMSRPLKTE"
     gene            752..1033
                     /locus_tag="VGM72_13945"
     CDS             752..1033
                     /locus_tag="VGM72_13945"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY4266415.1"
                     /translation="MLTAIDKDNSLNVRMSVREQHMAFVKETGVVRLGGPFLDAKGDM
                     AGSLIIFEATDMDAAKAWNARDPYMKAGLFQSTDIRPWKMTANFCEARL"
     gene            1033..1449
                     /locus_tag="VGM72_13950"
     CDS             1033..1449
                     /locus_tag="VGM72_13950"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEY4266416.1"
                     /translation="MAYWLFKTEPDTFSWEMQKKRGAKGEPWSGVRNFAAAKHMKSMK
                     KGDLGFFYHTGGEKQIVGIVEVIAEYKPDPTDETGRFGLVDVKAIKDVPKPVTLAQVK
                     ADPKLKTMVLVREARLSVQPVTDVEWKHICKLGGVK"
     gene            complement(1450..1941)
                     /locus_tag="VGM72_13955"
     CDS             complement(1450..1941)
                     /locus_tag="VGM72_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266417.1"
                     /translation="MTDYDDMKRGTAREWAAARIVLIGGFAIAIVVAGYFAWQRHADA
                     VAAQQEQAQQTAALARAQQPRPAFKETTQDEKAKGAMMFCAMELVNAKTMGIVPPFGQ
                     LASALPRKTATQGRYACIAATQVSKYVIEADLVCHQVTDPKCVRLHSIKSDDGTVLYQ
                     AKS"
     gene            complement(1969..>2419)
                     /locus_tag="VGM72_13960"
     CDS             complement(1969..>2419)
                     /locus_tag="VGM72_13960"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY4266418.1"
                     /translation="GRGFKMQGYDKGFYLGGSLFDRVTPNMKTYQEEIFGPVLQIARA
                     KDFDEAISLPSNHHYGNGVAIFTRDGDAAREFASRVQVGMVGINVPIPVPLAYHTFGG
                     WKRSAFGDVNQHGTEGVRFWTKIKTVTQRWPKGGMRENPDFNIPTMR"
BASE COUNT          474 a          734 c          724 g          487 t
ORIGIN      
        1 tctgcgcagc acgctcgcgc tgctcaagcc acatctctcc gcgaagaagc cgctggtgct
       61 ttgcgccaag ggtatcgagc acgacacgca cagactgatg accgaagtgc tggtcgaatg
      121 cattcccgat tgcgagcccg cgattttgtc cggtccttct ttcgccaaag acgtcgcgcg
      181 caatcttcca accgccgtca ccattgccgc gaaactcgaa atcgcgcggg cgctgcagga
      241 agcgttcggc catctcacct tccgccccta tgcgagcgat gatctcaccg gcgtcgcgct
      301 cggcggtgcg gcaaagaatg tttatgccat cgcttgcggt atcgttgccg gcatggggtt
      361 gggcgaaagc gcgcgcgccg cgctgctgtc gcgcagcttc gccgaattat gccgtctcgg
      421 tgaagcgatg ggcgcgcgca gcgaaactct gatggggttg tctggactcg gtgatcttgt
      481 gctcacggcc accagtgcat cctcgcgcaa ttacgcgttc ggactcgatg tcggcaaagg
      541 tctatcgcgc gaagaacttt acaagccggg tcgcccactg gctgaaggtg tcgcgacggc
      601 gcccgccctg gctgcgcgtg cgcatgaaca caaggtcgag cttcccattg cagagacgac
      661 atcggaaatc ctgcaaggca agatcgcact cgccgacgcc attccgcgtc tgatgtcacg
      721 ccctctcaag acggagtaga aaatgctttt cgtgctgacc gccatcgata aagacaattc
      781 attgaacgtg cgcatgagcg tgcgcgagca gcatatggcg tttgtgaagg aaaccggcgt
      841 cgtccgcctc ggcggtccgt tcctggatgc caaaggcgac atggcgggca gcctcatcat
      901 ctttgaagcc accgacatgg atgcagcaaa ggcgtggaac gccagagacc cctacatgaa
      961 agcgggcctg ttccaatcca ccgatattcg tccgtggaag atgactgcga atttctgcga
     1021 ggcgagactc tgatggcgta ttggctgttc aagaccgagc cggacacctt ctcctgggag
     1081 atgcaaaaga agcgcggcgc caagggtgaa ccgtggagcg gcgtgcgcaa tttcgcggcc
     1141 gccaagcaca tgaaatcgat gaagaagggc gatctgggct tcttctatca caccggcggt
     1201 gagaagcaga tcgtcggcat cgtggaagtc atcgctgaat acaaacccga tcccaccgac
     1261 gagacgggca ggttcgggct tgtcgatgtc aaagcgatca aggatgtgcc caagccggtg
     1321 acgctggcgc aggtaaaagc cgatcccaag ctcaagacga tggtgctcgt gcgcgaagcg
     1381 cggctttccg ttcagccggt taccgacgtg gagtggaagc acatctgcaa gctcggcggc
     1441 gttaagtgat cagctcttgg cctgatagag gaccgtgccg tcatcgctct tgatgctgtg
     1501 cagacgcacg catttcggat cggtcacctg gtggcacacc agatcggcct cgatcacata
     1561 tttcgaaacc tgtgtggccg cgatgcaggc atagcgtcct tgcgtggcgg tcttcctcgg
     1621 caacgcgctt gcgagttgcc cgaagggcgg tacgattccc atggtcttgg cgttcaccaa
     1681 ttccatggcg cagaacatca tggcgccctt ggccttttcg tcctgcgtgg tttccttgaa
     1741 cgccgggcgt ggctgttgcg ccctggcaag cgcggccgtc tgttgtgcct gttcctgctg
     1801 tgccgccacg gcatcggcat gccgctgcca ggcgaaataa ccggccacga cgatcgcgat
     1861 cgcaaagcct ccgatcagaa cgatgcgcgc agcggcccat tcgcgcgccg tgccgcgctt
     1921 catgtcgtcg tagtcggtca ttcctgatcc ccgataattt tctaagtctc agcgcattgt
     1981 cggaatattg aaatccgggt tctcgcgcat accgcccttg ggccagcgct gcgtcaccgt
     2041 cttgatcttc gtccagaaac gcacgccctc ggtaccatgc tggttaacat cgccgaaggc
     2101 cgagcgcttc cagccgccga aggtgtgata agccagcggg accggaatcg gcacgttgat
     2161 gccgaccatg cccacctgca cccggctggc gaattcgcgc gccgcatcgc cgtcgcgcgt
     2221 gaagatggcg acgccgttgc cgtagtgatg gttggagggc agggagatgg cctcgtcgaa
     2281 atccttcgcc cgcgcaatct gcagcaccgg cccgaagatt tcttcttgat aagttttcat
     2341 gttgggcgtc acgcggtcga acagcgaacc gcccagataa aagcccttgt catagccctg
     2401 catcttgaaa ccgcggcca
//
LOCUS       DASYEG010000333         4366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_278871, whole genome shotgun sequence.
ACCESSION   DASYEG010000333 DASYEG010000000
VERSION     DASYEG010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278871"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VGM72_13965"
     CDS             <1..266
                     /locus_tag="VGM72_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266419.1"
                     /translation="IRFRGSQLVDRPQLQRDDQFFSIGSERWTGDERSILSALQRSIF
                     IFNCNILDRLLDPAIALLRGLARKGSSRFLAAPGISGYGNAHA"
     gene            284..1759
                     /gene="hisS"
                     /locus_tag="VGM72_13970"
     CDS             284..1759
                     /gene="hisS"
                     /locus_tag="VGM72_13970"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY4266420.1"
                     /translation="MNEQTPFRPKARLPRGFRDRDEAELAAERRMLDTIRRVYSSYGF
                     QALETSSLEYADALGKFLPDSDRPNEGVFSLKDDDGQWLSLRYDLTAPLARYVAENFQ
                     NLPKPFRRYQVGTVWRNEKPGPGRFREFTQFDADTVGSASPAADAELLMMCADSLEAL
                     GLSRGEYLVKLSSRKLLDAVMDSAGVEIVKRSMVLRAIDKLDRLGIEGVRALLGSGRK
                     DESGDFTKGAELSGNAAGTILAFLTIRERGENFYSAARALIGDELWQRGAGKLVEITN
                     LCEASGYKSDRILIDPGIVRGLDYYTGAVFEAQLTFPVTNEAGEAVVFGSVGGGGRYD
                     DLVARFTGQSVPASGISIGVSRLLSALRVRAGAESFAVDPLIVVLVLDKAEIAHSFRL
                     ARTLRDAGFRSETYVGEAGMKAQLKYADRRGAAIAIIEGGDERARGEVTLKDLALGAE
                     LAKSTENRAAWVKDRPAQQSVKHADLIPAIRAILDGARARA"
     gene            1762..2928
                     /locus_tag="VGM72_13975"
     CDS             1762..2928
                     /locus_tag="VGM72_13975"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEY4266421.1"
                     /translation="MSAFPTYDAPLLKEIGAQTASVLDVFGAKAYARVEPPILQPAEI
                     FLDRSGEEIRRRTFVLTDPAGRELCLRPDLTIPVCRMYLASGGKFPARLSYHGPAFRA
                     SLSEPERPSQFLQTGVECLGVRDLEAADSEVLNLAVEGVRAAGLRQFSIQIGDLALFA
                     GLVDALDIPAQWRGRLKRHFWRPDYFRELLARLSNGGSVPGERYLAHLGTADDDQARA
                     ALSGLMDYLGGTPLGGRTREEIVDRLMEQAAEAAAIRLDKSAVALIEQVLSISGPASK
                     SITRIRALLKSHGVELDQPLERMQSRIASLARLGLADKHVSFAARFGRNMEYYTGFVF
                     ELWSRDSEGRVQVAGGGRYDTLLRTLGASADIPAVGCAIRSERVLAARRATGAL"
     gene            2930..3910
                     /gene="hisG"
                     /locus_tag="VGM72_13980"
     CDS             2930..3910
                     /gene="hisG"
                     /locus_tag="VGM72_13980"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEY4266422.1"
                     /translation="MAAPVLILAIPSKGRLQEQVMHYLGDAGLVLKQITGARDYRATI
                     AGEPGIDVKLMSSSEIANALGAGEIHFGITGEDLIRESIPDVDAKVALLQPLNVGFAD
                     VVVAVPRAWIDVVSMADLDDVCTAFHARHHRRLRVATKYLSLTRGFFAAHGIADYRIV
                     ESLGATEGAPAAGLAEAIVDITTTGATLSANDLKILQDGTILKSQAQLAASLGAKWSR
                     EALAAAARLTLRIAARELARNAYLLRAPLGRAWNKVLPALAKAGCTLLAQPSAGGGKH
                     VILCRREHLSDALNLLRESGVKGAVAAEPVDYVFGEVNPLIERLRARLKR"
     gene            complement(3941..>4366)
                     /locus_tag="VGM72_13985"
     CDS             complement(3941..>4366)
                     /locus_tag="VGM72_13985"
                     /inference="COORDINATES: protein motif:HMM:NF003974.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HEY4266423.1"
                     /translation="RYSGGQSYGGHIDGAIRPVAGTPHRVRTDLSATLFLTAPEDYDG
                     GELVVEDRSGVRSIKLAAGDMVLYPGTSVHHVESVTRGARLAAFFWIQSMVREEAKRD
                     ILFELDTALQQLGRDAPGHASLVQIAGCYHNLLRLWSDT"
BASE COUNT          762 a         1334 c         1400 g          870 t
ORIGIN      
        1 ggattcgatt tcgtggaagc caattggtcg ataggcctca attacagcgc gacgatcagt
       61 tcttcagcat tggctcagag cgctggactg gtgatgagcg ctcgatttta agcgccctgc
      121 aaagatcgat ttttatattt aactgcaata tcttagatag gcttcttgat ccggccatag
      181 ctctgctccg cggtttggcc cgaaagggca gctcgcgctt tcttgcggct cccggcatca
      241 gcggctatgg gaatgcccac gcctgagcaa ggtttttcga ttcatgaacg agcaaacgcc
      301 attccgcccc aaggcgcggc tgccgcgcgg tttcagagac cgcgacgagg cggagcttgc
      361 cgccgaacgg cgcatgctcg acacaatccg gcgtgtctac agctcctatg gtttccaggc
      421 gctggagacc tcttcgctcg aatatgccga tgcgctcggc aaatttctgc ccgattccga
      481 tcgtccgaac gaaggcgtgt tctccttgaa ggacgatgac ggtcaatggc tgtcgctgcg
      541 ttacgatctg accgcgccgc tggcgcgcta tgtggcggag aattttcaga atctgcccaa
      601 gccgttccgg cgctatcaag tcggcacggt ctggcgcaac gagaagcccg gcccggggcg
      661 gtttcgggag ttcacgcagt ttgacgcgga tacggtgggc tcggcctcgc ccgcggccga
      721 cgccgaactc ttgatgatgt gcgccgattc tttggaagcg ctcggcctca gccgcggcga
      781 atatttggtg aagctcagca gccggaaatt gctcgacgcg gtgatggatt cggcaggggt
      841 cgaaattgtc aaacgaagta tggtgctacg tgcgatcgac aagctcgatc gccttggcat
      901 agaaggcgtg cgcgctttgc tcggctccgg ccggaaagac gaatccggag atttcacgaa
      961 aggcgcggag ctttccggga atgccgccgg cacgatcttg gcatttctga cgatccggga
     1021 gcgaggcgaa aatttttata gcgccgctcg tgcgctgatc ggcgacgagc tctggcaaag
     1081 gggagcaggg aagctggtcg agatcacaaa tctatgtgag gcctcgggtt ataaatctga
     1141 ccgcatcctg atcgatcccg gcatcgtgcg tggactcgat tattacaccg gcgctgtctt
     1201 cgaagcgcaa ctcacttttc ccgtcaccaa tgaagcgggt gaggcggttg ttttcggttc
     1261 ggtcgggggc ggcggacgct atgacgatct ggtcgcgcgc ttcaccggcc aatctgttcc
     1321 ggcgtccggc atttccatcg gggtcagccg gctgctctct gcacttcgcg tgcgcgcagg
     1381 tgcggagtcc tttgccgttg atccgctcat cgtcgttctc gtattggaca aggcagagat
     1441 cgctcacagc ttccgattgg cgcgcacatt gcgcgacgcc ggtttccgat cggaaaccta
     1501 tgtcggcgag gccggcatga aggcgcagct caaatatgcc gaccggcgcg gcgccgccat
     1561 cgcgatcatc gaaggcggcg atgagcgcgc gcgcggcgag gtgacgttga aggatctggc
     1621 gcttggtgcg gaacttgcca aatcgaccga gaaccgcgcc gcctgggtga aggaccgccc
     1681 cgcgcagcaa tcggtgaagc acgcggacct cattcctgct atccgcgcca ttctcgacgg
     1741 cgcgcgggcc agggcgtgac catgtccgcg tttcccacct atgatgcgcc tctgctcaaa
     1801 gagatcggcg cccagaccgc ttcggtcctc gatgttttcg gcgcgaaggc ctatgcccgc
     1861 gtcgaaccgc ccattctcca acccgccgag attttccttg atcgttcggg tgaggaaatc
     1921 cgccgccgca ccttcgtgct gacggacccg gcgggccggg aattgtgctt gcggcccgac
     1981 cttacgatcc ctgtttgccg gatgtatctg gcaagcggcg gcaaatttcc tgcgcggctt
     2041 tcctatcacg ggcccgcgtt ccgtgcctcg ctgagcgagc cggaacggcc gagccaattc
     2101 ctgcaaaccg gcgtcgaatg cctgggcgtg cgcgatcttg aagccgccga cagcgaagtg
     2161 ctcaaccttg ccgttgaagg cgtgcgggcg gcgggcctta ggcaattctc catccagatc
     2221 ggtgatctcg cgctgtttgc cggtctcgtg gatgcgctcg atattccggc gcaatggcgc
     2281 ggccgcttga agcggcactt ctggcgtccg gattattttc gcgaattgct cgcgcggctt
     2341 tccaatggcg gttcggtccc gggtgagcgc tatcttgccc atctcggcac cgcggacgac
     2401 gatcaggccc gcgcggccct ttcgggtttg atggattatc ttggaggcac gccgctgggc
     2461 gggcgcacgc gcgaggagat cgtcgaccgg ctgatggagc aggccgctga agccgccgcc
     2521 atccggctcg acaaatccgc cgtcgcgctg atcgagcagg tgctttcgat atcggggcct
     2581 gcatcgaaga gcatcacccg gatacgcgcg cttctgaaga gccatggcgt ggaattggat
     2641 cagccgctcg aacgcatgca atcgcgcatc gcgagtcttg ccagactggg cctcgccgac
     2701 aagcatgtga gttttgccgc ccgcttcggc cgcaacatgg aatattacac cggcttcgta
     2761 ttcgagctgt ggtcgcgcga ttccgaagga cgcgttcagg tggctggcgg cgggcgctat
     2821 gacacgctgc tgcgcacgct gggcgcgtcc gccgacatcc ccgcggtcgg gtgcgccatc
     2881 cgcagcgaac gcgttctggc tgcgcgccgc gccacgggag cactctgaga tggccgctcc
     2941 agtcttgatc ctggcaattc cctccaaggg ccgcctgcag gaacaggtga tgcattatct
     3001 cggcgatgcc ggcttggtgc tgaaacagat tacgggcgcg cgcgattatc gcgcgacgat
     3061 cgcaggtgaa cccggcatcg acgtgaagct gatgtcatca tcggagatcg cgaatgcgct
     3121 gggggccgga gaaatccatt tcgggatcac gggcgaggac ctgatccgcg aatccattcc
     3181 cgatgtggat gccaaggtcg cgttgttgca gcccttgaac gtcggttttg ccgatgtggt
     3241 ggtggcggtg ccgcgcgcct ggatcgatgt cgtcagcatg gcggacctcg acgatgtctg
     3301 cacggcgttt catgctcgcc atcatcggcg gctgcgcgtg gcgaccaaat atctgtcact
     3361 gacgcgcggc ttcttcgccg cacatggcat cgccgattac cgcatcgtgg aaagcctcgg
     3421 tgcgacggag ggcgcgcccg cggccggact tgccgaagcc atcgtcgata tcacgaccac
     3481 gggtgcgacg ctcagcgcca acgacctcaa gatcctccag gacggcacca tcctgaagag
     3541 ccaggcgcag cttgccgcgt cacttggagc gaaatggagc agggaagcgc tcgccgccgc
     3601 ggcgcgcctc acgctccgca ttgccgcgcg cgaactggcg agaaatgcgt atctcttgcg
     3661 cgctccgctc ggccgcgcgt ggaacaaggt tcttccggct ctggcaaaag cgggctgtac
     3721 gctgcttgcc caaccctctg ccggcggcgg caagcatgtg atcctctgcc ggcgcgagca
     3781 tctgtcggac gcgctgaatt tgttgcgtga atccggcgtg aaaggggcag tcgcggccga
     3841 gccggtggat tacgtgttcg gcgaagtcaa tccgctgatc gaacgcttgc gtgcccggct
     3901 caagcgttag ctttcccgcc tgggtcttaa gacggcaaga tcaggtatcg gaccacagac
     3961 gcagcagatt gtgatagcag ccggcgatct ggaccagcga ggcgtggccg ggtgcatcgc
     4021 ggccaagctg ttgcagcgcg gtgtcgagct cgaacaggat gtcgcgcttg gcctcttcgc
     4081 ggaccatgct ctggatccag aagaacgccg ccagccgcgc tccgcgcgtg acggactcca
     4141 catggtgcac gctggtgccg gggtagagga ccatgtctcc ggcagcaagt ttgatgctgc
     4201 ggaccccgct tctgtcttcg accaccagtt cgccgccgtc ataatcctcc ggcgcggtca
     4261 ggaacaaagt cgccgaaaga tccgtgcgta cgcgatgcgg cgtgccggca accggccgga
     4321 tcgcgccgtc gatatgaccg ccataggact gcccgccgga atagcg
//
LOCUS       DASYEG010000334         2752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3903230, whole genome shotgun sequence.
ACCESSION   DASYEG010000334 DASYEG010000000
VERSION     DASYEG010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3903230"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="VGM72_13990"
     CDS             <1..1398
                     /locus_tag="VGM72_13990"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY4266424.1"
                     /translation="LIFLAASLNTITTYVLLEQEAWFEKEAKFLPLLIKPGMVALDIG
                     ANAGVYSLALARSVGPSGKVYAYEPGAAPRRLLEKSRNANGFDNLQIVPAAVSDRSGR
                     GHLEPSVSSELASLGGSALGESVSVTSLDEEDASRNWGSPDFVKIDAEGEERRILEGG
                     RGFFARHSPLVMFEFKAADVVDKEIAEAFRASGYRIFRLLPGAPFLVPVNASENFDSY
                     ELNLFAAKPDRIAALARAGWLSDEMREWSPGDSARTQAMDLWAAQAFGPALARLPPPP
                     MGSPYRDALAGYAVWRSQTADLALRHAALRFSYGVLRELCRASGQAAHLSTFARVAWD
                     LGERRNVIAILEAIVAQGREHTGLAQPFWPVNPRFDAIAPQANVGQWFLISVLEQLER
                     ASAHSSMFLGEGLNLDWLGRQPFASTEITRRNVLKQLRAGKRLAVPEKLCRPASDHRN
                     SELWRSGLIPNTLAS"
     gene            1417..1857
                     /locus_tag="VGM72_13995"
     CDS             1417..1857
                     /locus_tag="VGM72_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4266425.1"
                     /translation="MQYLHTMIRIADIGKSLAFYCGLLGLKEMSRIENEKGRFTLIFL
                     AAPGDEDLARERKGPMVELTYNWDREDYTGGRNFGHLAYRVDDIYAICKTLMDGGITV
                     NRPPRDGHMAFVRSPDGISVELLQRGPALPPQEPWASMPNTGKW"
     gene            1900..2463
                     /locus_tag="VGM72_14000"
     CDS             1900..2463
                     /locus_tag="VGM72_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY4266426.1"
                     /translation="MALHQGRRIVTGHDEQGRSIVISDGPPANVVPNPSDPERGHINF
                     WRTASMPADNGDPADPMAGPPCPLPPPKGGTMFRFFQIAPEKNEAALSPEERNARMAR
                     MFEAAGAGGARISMGRHASMHRTDSLDYIVLLKGEVTALLDVGEVAMKPFDVLIQRGT
                     NHGWVNYGDEPALLVAVLVDAEPLART"
     gene            2580..2656
                     /locus_tag="VGM72_14005"
     tRNA            2580..2656
                     /locus_tag="VGM72_14005"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2614..2616,aa:Met,seq:cat)
BASE COUNT          497 a          883 c          855 g          517 t
ORIGIN      
        1 ctcatctttc tcgccgcttc gctcaacacg atcacgacct atgtcctgct tgaacaggaa
       61 gcgtggttcg aaaaggaagc gaaattcctc ccgcttctga tcaagccagg catggttgcg
      121 ctcgatatcg gggcgaatgc cggggtctac agccttgcgc tggcccgctc cgtgggtccc
      181 agcggcaagg tctatgccta tgaaccggga gccgcgccgc gccggcttct ggagaaaagc
      241 cgcaacgcca acggcttcga caatcttcag atcgtgccgg ccgccgtttc ggaccgctct
      301 gggagaggcc atcttgaacc cagtgtctcc agcgagctcg cgagtctcgg cgggagcgct
      361 ttgggcgaat ccgtcagtgt gacctcgctc gatgaagagg acgcgtcgcg caattggggt
      421 tctccggatt tcgtcaagat cgacgccgaa ggcgaggaac ggcgcatcct ggaaggcggc
      481 cgcggctttt tcgcacggca ctcgccgctc gtgatgttcg aattcaaagc cgccgatgtc
      541 gtggacaaag aaatcgccga ggcattccgg gcctcgggct atcgtatctt ccggcttctt
      601 cccggcgcgc cgttcctcgt tccggtgaac gcctcggaga atttcgacag ctatgaactg
      661 aacctgttcg cggccaaacc ggaccgcatc gccgcgctcg cgcgcgcggg atggctcagc
      721 gacgagatgc gggaatggtc gcccggcgat tcggcgcgca cccaagccat ggacctgtgg
      781 gccgcacagg ccttcgggcc ggcgctcgca aggctgcctc cgccgcccat gggttccccc
      841 taccgcgacg cgctcgccgg ttacgcggtg tggcgctcgc aaacggcaga ccttgcgctg
      901 cgccacgccg cacttcgttt cagttatggc gtgctgcgtg agctttgccg cgcgagcggt
      961 caagccgctc acctctcgac ctttgcgcgt gtcgcctggg atctcggcga acgcaggaac
     1021 gtcatcgcca tcctcgaagc catcgtcgcg cagggacgcg agcataccgg tctcgcccag
     1081 ccgttctggc cggtcaatcc ccgcttcgat gccatcgcgc cgcaagccaa tgtcgggcaa
     1141 tggttcctga tctccgtcct ggagcaattg gaacgggctt cggcccattc ttccatgttc
     1201 ctgggcgagg gactgaatct ggattggctg ggccggcagc ctttcgcctc gaccgagatc
     1261 acgcgccgca atgtgctgaa gcaattgcgt gccggcaaac gcctggctgt gcccgaaaaa
     1321 ttgtgccggc cggcgagcga tcatcggaac agcgaactct ggcgctccgg attgatcccc
     1381 aatacgctgg cgtcctgata aacggagccg tcgattatgc aatatctcca caccatgatc
     1441 cgcatcgccg atatcggcaa aagcctcgct ttctattgcg gcttgctcgg cctgaaggaa
     1501 atgagccgca tcgaaaacga aaaggggcgg ttcacgctga tcttcctggc ggcgccgggc
     1561 gatgaggacc tggcgcgcga gcgcaaaggg ccgatggtgg agctgaccta caattgggat
     1621 cgcgaagatt acaccggcgg gcggaatttc gggcatctcg cctatcgcgt ggacgacatc
     1681 tatgccatct gcaagacgct gatggacggg ggaatcaccg tcaatcgtcc gccgcgcgac
     1741 gggcacatgg catttgtgcg ttcgccggac ggcatctccg tcgagctgct gcaacgcggc
     1801 ccggcgctgc cgcctcagga gccttgggct tccatgccca atacaggcaa atggtagggc
     1861 tgcgtctgcg atcatccgta taaagatagg gtgagcgaca tggcattgca ccagggccgg
     1921 cgcatcgtga ccgggcatga cgaacagggc cggtccatcg tcatctccga tggcccgccc
     1981 gcgaatgtag tgcccaaccc gtccgatccc gaacgcggcc acatcaattt ctggcggacc
     2041 gcatcgatgc ccgcggacaa tggcgatcct gccgatccga tggcagggcc gccctgcccg
     2101 cttcccccac ccaagggcgg caccatgttc cgcttcttcc agatcgcgcc ggagaagaac
     2161 gaggccgctc tttcgcctga ggagcgcaac gcccgcatgg cacgcatgtt cgaagcggcg
     2221 ggcgcgggcg gggcgcgcat ctccatgggg cggcatgcct cgatgcatcg caccgattcg
     2281 ctcgattaca tcgtgctctt aaagggcgaa gtgacggcgc ttttggatgt gggcgaggtg
     2341 gccatgaagc ccttcgacgt gctgatccag cgcggcacca accatggctg ggtaaattat
     2401 ggcgatgagc ccgcgcttct ggtggcggtg ctggtggatg cggagcctct ggcgcgaacg
     2461 tgaagggccg atgccccgca tcgggccgcg ctccgccgct atacgagaat tcacccgcat
     2521 ggggtggaca gacggcggct cgtaacctat atcccctcac cctcctcgga gggccgtgac
     2581 gcggggtgga gcagcccggt agctcgtcag gctcataacc tgaaggtcac aggttcaaat
     2641 cctgtccccg caaccaaatt tcgccgtcaa tgcgtgtttt gcgaagaatg gccgcacctt
     2701 gaaggcggcc attcttctat tgctccccgt aaaacgccaa gactgagccg ct
//
LOCUS       DASYEG010000335        18368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_280020, whole genome shotgun sequence.
ACCESSION   DASYEG010000335 DASYEG010000000
VERSION     DASYEG010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 18368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18368
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280020"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            411..1493
                     /locus_tag="VGM72_14010"
     CDS             411..1493
                     /locus_tag="VGM72_14010"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF016210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HEY4266427.1"
                     /translation="MTTRAIPCVLMRGGTSRGPYFLASDLPSDPAERDAVLLQVMGTG
                     HELQVDGIGGGHPLTSKVAIVSPSRVPGADVDYLFAQVNVHERLVDISPNCGNMLAGV
                     GPFALEAGIVAAQDGMSTVHIFNVNTQKLIEARIETPGRRVEYEGHTAIDGVPGTAAP
                     VHLAFLDAAGAKTGRLFPTGNPTDRVNGVAMSLVDAAMPIMAVRAADVGKTGYENPAD
                     FAADHPFMQRLEALRLEAGRLMGLGDVRGLVIPKPVLVARPRSGGTLAARYFMPHSCH
                     NAFAITGAVAVGTAAVTPGTIIAEIAGTPEMPVDVVIEHPSGRMQVRLEQRPGEKAPV
                     AYLVRTTRRLFEGRVLVNVREAAAAG"
     gene            complement(1490..2284)
                     /locus_tag="VGM72_14015"
     CDS             complement(1490..2284)
                     /locus_tag="VGM72_14015"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY4266428.1"
                     /translation="MLISLNTRRLHYDLIGPETGRVVCFAHALAADSGMWSEQIPAVL
                     ALGRRVLRIDMRGHGGSDPVPGDYTLAELASDIVAVLDAADIGGADYVGLSIGGMIGE
                     ALALHHSSRVSSLMLCETPPASLRNAQEIWGPRIAAVKKAVSLEPIADATMERWLTPD
                     FKARHPVRWRQIRDTVAATSVPGYCGAVAALSSFDFGAELPRLRVPALVLYGEEDRAT
                     SHEENQRLASLIPGGRFVAVSGARHLPNIEDPARFNRILTDWLSRV"
     gene            complement(2336..2569)
                     /locus_tag="VGM72_14020"
     CDS             complement(2336..2569)
                     /locus_tag="VGM72_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266429.1"
                     /translation="MSPDKSKSDEKTKSEEGVQGEGDYKSARVYKKDIEGFIQQKGGE
                     IPQMAKDAEKAVEGPEGADLTKAEEKGKSKARH"
     gene            2803..3381
                     /locus_tag="VGM72_14025"
     CDS             2803..3381
                     /locus_tag="VGM72_14025"
                     /inference="COORDINATES: protein motif:HMM:NF022150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HEY4266430.1"
                     /translation="MPRNDEHKSTSNRGFASMDRAKQRQIASKGGQSVPPEKRSFSQD
                     PQLAAQAGRKGGESVPAEGRSFSQNRELAAEAGRKGGQARGASTHRGKSNTQAEPENG
                     ETEASRQGGTFSEDRDTAAEGAEKAGLPKNQRAQDERSRFPAPGAANDDQREDLEERN
                     DRHGTQDLQRDDFAQNPARHGPSSGKEHPHGV"
     gene            complement(3442..5160)
                     /gene="ilvD"
                     /locus_tag="VGM72_14030"
     CDS             complement(3442..5160)
                     /gene="ilvD"
                     /locus_tag="VGM72_14030"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY4266431.1"
                     /translation="MPTYDKSKLPSRHVTEGPERAPHRSYYYAMGLTDEEIHRPFVGV
                     ASCWNEAAPCNIALMRQAQSAKRGVKEAGGTPREFCTITVTDGIAMGHEGMKSSLVSR
                     EVIADSVELTVRGHCYDALVGIAGCDKSLPGMMMAMLRLNVPSVFLYGGSIFPGKFHG
                     KDVTVVDVFEAVGKHSAGAMPESELHELECVACPGAGACGGQFTANTMACVSEAIGLA
                     LPYSAGPPAEVTSRDDYALRAGETVMALLANNIRPRDICTRAAFENAATIVAATGGST
                     NAALHLPAMAHEAGIAFDLFDVAAIFKRTPYLADLKPGGRFVAKDMWEAGGMPMLMRT
                     LLDGGYLNGDCMTVTGKTIAENLSGVTFNAAQEVMRPATNPLAPTGGVVGLSGNLAPE
                     GCIVKVAGLKHLHHRGPVRVFDCEEDAFAVVQARNYKEGDVIVIRYEGPKGGPGMREM
                     LSTTAALYGQGLSDKIALITDGRFSGATRGLCIGHVGPEAAEGGPIGLLKEGDIVVID
                     AEKGTIDVELSEAELVRRRSVWKPKAAAFGSGALWRFAQNVGPARRGAVTHPGGAVEL
                     RCYADI"
     gene            complement(5690..6580)
                     /locus_tag="VGM72_14035"
     CDS             complement(5690..6580)
                     /locus_tag="VGM72_14035"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEY4266432.1"
                     /translation="MPASPASTATFQGLILTLQNYWAAQGCLILQPYDMEVGAGTSHP
                     ATTLRALGPKPWRAAYVQPSRRPKDGRYGENPNRLQHYYQYQVILKPSPIEVQDIYLD
                     SLKTLGIDPKRHDIRFVEDDWENPTLGAWGLGWEVWCDGMEVTQFTYFQQVGGFDCAP
                     VSAEITYGLERLAMYVQGVENVYDLRFNEQFRYGEVFHQAEIEYSKYNFEHSDAEILK
                     RHFEDAEKECKNLLDAGLALPAYDQCLKASHNFNLLDARGVISVTERAAYIGRVRTLA
                     KAACEAWLASGQGDYTPKRA"
     gene            complement(6633..6914)
                     /locus_tag="VGM72_14040"
     CDS             complement(6633..6914)
                     /locus_tag="VGM72_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266433.1"
                     /translation="MKFGTDLIGWAAAAILLLTLGRQVYTQWRDRTSQGVSRWLFVGQ
                     CAASVAFVVYSWLLGSWIFVVTNALILLTAVVGECIYVRNRREKKKTPA"
     gene            complement(6911..7630)
                     /locus_tag="VGM72_14045"
     CDS             complement(6911..7630)
                     /locus_tag="VGM72_14045"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY4266434.1"
                     /translation="MSGELATLDRFLGGRLLAAQPRNGFRAGHDTVLLAAAVPAHASA
                     QVLELGAGAGIASLCLAARVPGVRVLGIDIDAGLVVLANENAARNGLDGRVCFEPGDA
                     AQFSGGRQFDHVFFNPPFHPASGQQSPNLSRARAMRGEHALSQWMRVALGLTRPGGTV
                     TAITRADRAAELLRVKDGAVLFPLFPHEGDAPKRAIVQIVKDGTAPFREAAGMVLHER
                     DGRNTNAAEAVLRQGEELRLV"
     gene            complement(7627..7860)
                     /locus_tag="VGM72_14050"
     CDS             complement(7627..7860)
                     /locus_tag="VGM72_14050"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEY4266435.1"
                     /translation="MKELLRTNNFVYLSFAQAVLKDAGVEVLLFDSHMSVMEGSLGVL
                     PRRLMVADDDFAHARKILAEAEPLDSIPDVDLA"
     gene            7970..8989
                     /locus_tag="VGM72_14055"
     CDS             7970..8989
                     /locus_tag="VGM72_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY4266436.1"
                     /translation="MSTNTRETASLPSSINPVERLALLTGTDLNGVDAVIHSRMGSEV
                     PLIPQLGQHLVDSGGKRLRPLITLAAARHYGYDGDAHVKLAAAVEFIHTATLLHDDVV
                     DESALRRGLPAANVLWGNKPSILVGDFLFSRAFQLMVEIGSPAVLSVLANASAVIAEG
                     EVMQLRSAKNLAIGENEYLKVITAKTAALFAAAAEAGAMAAGAPPAQYEALRDYGRNL
                     GIAFQLVDDALDYSGRQAVMGKSVGDDFRESKVTLPVILTLQRASDEERRFWRKTIEV
                     GVQEEGDLTRAIELLERGGALAETIERARLYARWARDALNVCAQSEIKAALADIADFV
                     VERAY"
     gene            complement(8986..9873)
                     /locus_tag="VGM72_14060"
     CDS             complement(8986..9873)
                     /locus_tag="VGM72_14060"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF011202.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEY4266437.1"
                     /translation="MRLFAPAKINLFLHAGEKRADGYHALESLVAFAQIGDRLDIGAA
                     STLTLDVSGPFANRLVRNDDNLVLRAARALAANASGAQRGAHIALEKNLPVAAGLGGG
                     SADAAAALRGLNAYWNLGLAESALLEIAATLGSDVPACLLSRTCWMEGRGEHVREMPP
                     MPQFPLILINPGVLVPTGAIFAGLNARTGIGAIEPPGTMQSVWDLVAYLADAANDLEA
                     PASRYAPLIEDVLAALDREPGCVLAQMSGSGATCFGLFEQEQFALGAAERIAQDHREW
                     WVRATRIAPPDIGAPQPAA"
     gene            complement(9890..11608)
                     /locus_tag="VGM72_14065"
     CDS             complement(9890..11608)
                     /locus_tag="VGM72_14065"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4266438.1"
                     /translation="MQLKTLLALASSLTALSLGACSTAGSMMSASAPVAGEASQNSLA
                     GTYLAANFAAAQGDVKAATGFYAHSLKDDPGNADLLERTFLFAAEAGDIDQAIGLTDR
                     VLALDTDNRPAHLVREVGALVKKDYAGVIKDVDQPAPGLFAALTNRVIDAWARAGSHD
                     IDGALASLDTLSAQRGVDGLRLMHRALILDFAGRDKEADDSYKQAVSVMGTGPRAADA
                     YGRFLQRHGRIDEAKAFYERMQSENPGNPVAGWALKDIAAKKIPQPLVNSPAEGVSEA
                     LFGIAASLNDQRSADVAILYLNLTLYMRPDFDLAKVLLASRYEAMQKYDMANSLYAKI
                     QPVSLYYGMTQVQAAINDGRLGQVNAGIAKMKALAAMQPKESDVWTALGDLFRSADQY
                     NDAVAAYDKAIAGIGPGDRRLIGLYYARGISLERSNHWDAAERDFRAALKLNPDRADV
                     LNYLGYSWVDKGQNLEEAVSMLEKARALRPLDGMIADSVGWAYYRLGRYQDAARALEE
                     AVQLTPGAADVNDHLGDAYWRVGRKIDARFQWQHALQMNPDVKQKPVIERKLQFGLDP
                     VSASGS"
     gene            complement(11663..13321)
                     /locus_tag="VGM72_14070"
     CDS             complement(11663..13321)
                     /locus_tag="VGM72_14070"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF017038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEY4266439.1"
                     /translation="MSAARESMEFDVVIVGAGPAGLAAAIRLKQRAQREGREIGVCVI
                     EKGAEAGAHILSGAIVDPIGLNALIPDWKARGAPVETQVTEDRFYFLGPAGHIRLPHL
                     LMPPLMSNRGNYIVSLGLLCRWLAEQAEALGVEIYPGFAAVDVELGESGEVAGVITGD
                     LGVARDGHHKPNYTPGMALRGKYTLIAEGARGSLAKQIIARYGLAKDSAPQTYGLGLK
                     ELWELPKAKHRPGLVMHSMGWPLDNHTGGGSFLYHYGDNLAAVGFVVHLDYRNPYLSP
                     FDEFQRFKTHPFIRPMLEGGRRIAYGARAITEGGFQAVPKLAFPGGALIGCAAGFVNV
                     PRIKGSHNAILTGMMAADAAFDAIAQERAGDRLTQYEDAYRISSVCKDLKRVRNVKPL
                     WSRYGTVLGVPVGALDMWFNQLFGFSPFGTLRGKGPDYAQLIPAKDARPIAYPKPDGS
                     VSFDKLSSVFLSGTRHDEDEPVHLKLKDPALPIAVNLPVYAEPAQRYCPAGVFEVVTG
                     DSGPRFVINAANCVHCKTCDIKDPAQNIIWCAPEGGDGPAYGTM"
     gene            13383..14273
                     /locus_tag="VGM72_14075"
     CDS             13383..14273
                     /locus_tag="VGM72_14075"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY4266440.1"
                     /translation="MAQIAYIRPMADPTRERSLAALRWLMEAGADEAIAEEPVNRFAQ
                     RARITPDAEDAVSARSGERPGGTSLMRAPLAQSEGMKRTASAEAVSLSTAPGAARALA
                     ASCMNLDALRAALLNFDGCELKRHAKNTVFADGNPLGRIMLIGEAPGRDEDEQGLPFV
                     GRAGKLLDRMLSAIGLDRSKVYITNVLNWRPPQNRDPSPDEAAACLPFLHRHIELAEP
                     QILILLGAVAVRHVLGLTEGIMRARGRWQLYQSVHLGRAIPVMPTLHPAFLLRQPVAK
                     RLAWRDLLEISERIEQLGLA"
     gene            14318..16033
                     /locus_tag="VGM72_14080"
     CDS             14318..16033
                     /locus_tag="VGM72_14080"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY4266441.1"
                     /translation="MKKFAAIALAGFCCIAAFASARAADQVASSTILSPADVKTYGEI
                     FAAQKTGQFAKADRLIAKLEDTSLLGYVLQDRYLGAHYRSKFGELKSWLTEYGDLVGA
                     DGIYKLAVKRAPKKTAVPVPARAHWRGAAGDGLAFDNMKLQSEAAERIAQQLRSLARD
                     DRPEDADALVKKLAATRSGLQQGDIDRLAAYVATAYLGEMQDQAALSRAEEIIARHGI
                     SAVQCEWTAGLAAYRLGRFEDAAHHFEAVMQSASAPRTYAAAAFWAARSWTGTGEAER
                     VLTLYERAASEGADTFYGLLATRLLGRDMKADFSEPALDSVSFAGLMLEKPAHRAVAL
                     WQIGHAEAVEAELARAFGQMSPDLDPAFAALARSLGAPALELRAAETAAPTVRLTSLY
                     PVPPYEPQGGYALDQAVVLAFARQESRFEPGALSKAGARGVMQIMPQTAVTITHDSSL
                     AGRNKDRLDDPTYSITLGQNYLLDLIDRQNGNLFSIAAAYNAGEGNLTKWQSQRADDS
                     DPLLFIETIPLAETRDYVKRVMTNVWMYRLRLGEAPDGLDAAAAGSWPTYTQAVQAGT
                     ASVSP"
     gene            16040..16582
                     /gene="moaB"
                     /locus_tag="VGM72_14085"
     CDS             16040..16582
                     /gene="moaB"
                     /locus_tag="VGM72_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HEY4266442.1"
                     /translation="MAEVRPREFIPVRIAVLTVSDTRTRADDVSGDTLEARVREAGHH
                     VAGRAIVPDEIARIAEQLIVWIADPEIDVVISTGGTGLTGRDVTPEAFRSVFEKEIDG
                     FPAVFHRISYDKIGTSTIQSRACAGVAKGTYLFALPGSPGAVKDGWDGILKAQLDYRH
                     RPCNFIEIMPRLEEHLRRAK"
     gene            complement(16584..17372)
                     /locus_tag="VGM72_14090"
     CDS             complement(16584..17372)
                     /locus_tag="VGM72_14090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM/exosortase system-associated
                     acyltransferase"
                     /protein_id="HEY4266443.1"
                     /translation="MTLHNILQFAEIADTAENNLPLAGNADSTTGAGTLLERFQSQFD
                     VLPANTLERVRIAQRIRYQVYCVEHSHEKADDPDGEEMDEFDSHAAQSLLIHRAAKTP
                     LGTVRLILPRPDALHRSFAVQRVMDAAALSEFQKFPLQTTGEVSRFSISRQFRRIAGL
                     SNGGADIDLLGSSGPLMRLGLIQALVRMSREHGITHWCAAMEPTLLRMLSAMAIRFRP
                     IGPLVEYHGLRQPAYCVLADVLKAVRDERPAFWSILTEDGARIH"
     gene            complement(17801..18040)
                     /locus_tag="VGM72_14095"
     CDS             complement(17801..18040)
                     /locus_tag="VGM72_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266444.1"
                     /translation="MQKSDTLVDTAELRKQASEARYLAAAFKDRATVSDLLSYATALE
                     RDAARWEEVVRKTGATSVASYFSAWLNIFAVRGQH"
     gene            complement(18129..>18368)
                     /locus_tag="VGM72_14100"
     CDS             complement(18129..>18368)
                     /locus_tag="VGM72_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266445.1"
                     /translation="PVVVQDGGDYDAPPPDYLPPPDAPPPEQSWYHCSNPDGFYPYVR
                     NCEGRWEAVPVTPDGGPPQQGPAQQGRAPGAPQER"
BASE COUNT         3507 a         5893 c         5606 g         3362 t
ORIGIN      
        1 tgaaacaagc acgtgccgaa tgccggggtt gcagtcaaac ttgggtggcc cataacgcca
       61 tgggcctagc gtcccagcat cacgaaaaaa ccggccatcc tgttgaagtt cacgtgactt
      121 ataacagcga cgcgcgcccc cgccggaatg accaaaagcg cccggcggag atcggtgcgc
      181 ggtaatcgca gccggtcatc ctgacctgcg gatttgacac atgcctgaaa gagaagggcc
      241 ccgcgcaata acgcgcgcga ggccctgaac aacacgctca accaacttgt gaggcaggtc
      301 ggcgccatta ccgtctcacg gctggcgtta cgagccggtt aagtcctccc gacgcagtgt
      361 ttttaacgta cggcttgcct gatgaggcgt ttttccggca aaagagcgtt atgaccacgc
      421 gcgccattcc ctgcgtcctg atgcgcggcg ggacctcacg cggtccctat ttcctcgcgt
      481 ctgacctgcc atccgatccc gcggagcgcg atgcggtcct actccaggtg atgggcactg
      541 gccatgaact ccaggtggat gggattggcg ggggtcaccc attgaccagc aaagtcgcaa
      601 tcgtcagccc atcgcgtgtg ccgggcgccg atgtcgatta cctcttcgcg caagtgaatg
      661 tgcacgagcg actggtggat atctcgccca attgcggaaa catgctggcg ggcgtaggcc
      721 ctttcgctct cgaggcgggc atcgtcgcag ctcaggatgg catgtccacc gttcacatct
      781 tcaatgtgaa cacccaaaag ctcatcgagg cgcgtatcga gacacctggg cgccgcgtcg
      841 aatatgaggg ccacaccgcc atagacggcg ttccggggac agccgcgcca gtccatctgg
      901 cgttcctcga tgccgcgggc gctaaaaccg ggcgattgtt cccgaccggc aatcccacgg
      961 accgggtgaa cggtgttgcc atgtcacttg tggatgccgc catgccgatc atggcggtgc
     1021 gcgcggccga tgtcggcaag accggctacg agaaccccgc cgatttcgcc gccgatcacc
     1081 ctttcatgca gaggctcgaa gccctgcgtc tcgaagccgg ccggctgatg gggcttggcg
     1141 atgtccgggg gctcgtcatc cccaagccgg ttctggtggc gcggcctcga agcggcggta
     1201 cgctcgccgc gcgctatttc atgccgcact cctgtcacaa cgcgttcgcc atcaccggtg
     1261 ccgtggcggt gggaacggca gccgtgacac cgggcaccat catcgccgaa attgccggga
     1321 ctcccgaaat gccggtggat gtcgtgatcg aacatccatc gggccggatg caagtgcgcc
     1381 tggaacagcg tcctggggaa aaagccccgg tcgcttatct ggtacgcacc acgcggcgcc
     1441 ttttcgaggg acgcgtgctt gtgaatgtac gcgaggcggc ggcggccggt taaacgcggc
     1501 tcagccaatc ggtcaggatg cgattgaacc gcgccggatc ttcgatattc ggcagatgac
     1561 gggcacccga aacggcgaca aagcggccac ccgggatcag agacgcaagg cgttgatttt
     1621 cttcatgcga ggtagcccgg tcttcttcgc cataaaggac cagtgccggc acgcgcaggc
     1681 gtggaagctc ggcgccgaaa tcgaatgatg aaagcgccgc aacagcgccg caataacctg
     1741 ggacggacgt tgccgccacc gtgtcgcgga tttgccgcca gcgcacgggg tgccgcgctt
     1801 taaaatcggg tgtgagccag cgctccatgg tggcatccgc gataggttcg agcgagacgg
     1861 ccttcttcac cgcggcgatt cgcggtcccc aaatctcctg cgcattcctc agggaggcgg
     1921 gcggcgtttc gcacagcatt aacgaggaca cacgcgagga atgatggagc gcaagcgctt
     1981 ctccgatcat gccgccgatg gaaaggccga cataatcggc cccgccgatg tccgcggcgt
     2041 caagaaccgc aacgatgtcg gaggcgagtt ctgcgagcgt atagtcaccc ggcaccggat
     2101 cgcttccacc atggccgcgc atatcgatcc gcagcacccg ccgccctaaa gcgagtacgg
     2161 ctgggatttg ttcgctccac atccccgaat cggcggccag cgcatgggcg aaacaaacca
     2221 cacgccctgt ttccggcccg atcagatcgt aatggagacg gcgtgtattc aatgagatca
     2281 gcatcgcgga ttgctatcgc ggggcctccg ctcatgcaaa tgccacagaa ttctttcaat
     2341 gcctggcttt ggatttaccc ttttcctcag cctttgtgag atctgctccc tctggtccct
     2401 cgaccgcttt ttcggcgtcc ttggccattt gaggaatttc tccgcccttt tgctggatga
     2461 acccctcaat gtctttcttg tagacgcgag cggatttgta gtcgccttcg ccctgcacgc
     2521 cctcctcgct cttggttttt tcgtcgcttt tggatttgtc cgggctcatc gcgcttatcc
     2581 ttctgatcac ccaagcggaa cgatccacag aaccgaaagt tgctgagagg cctcgtagac
     2641 catggaaatt gcataacaat tgcgcatttc cggccgagtt cgagtttctt gcacaattat
     2701 cgatagttcg cggaacggct gagcgctcgc ggtcgttatc caatgaacca gcggcagggc
     2761 gagtgtttcc gtgcctgacg ttataacgag aaaggacatc tcatgcctcg aaatgacgag
     2821 cacaagagca cgtccaatcg cggctttgct tcgatggatc gagccaagca gcgtcaaatc
     2881 gcaagcaagg gcgggcagag tgtgcctccc gaaaaaagaa gcttttccca ggacccgcaa
     2941 ttggcggcgc aggctgggcg caagggtggc gaatccgtac cggccgaagg gcgcagcttc
     3001 tcacaaaacc gcgagctggc cgcagaagcc gggcggaaag gcgggcaggc ccgcggtgcg
     3061 tccacacatc gcggaaagtc aaacacccag gccgagccgg aaaacggtga gaccgaggca
     3121 agccgccaag gtgggacgtt ttcggaagat cgcgacacgg ccgccgaagg cgcagagaag
     3181 gccgggttac cgaaaaatca gcgcgcgcag gacgagcgta gccgtttccc cgcccccggt
     3241 gcggcaaatg acgatcagag agaagatctc gaagaacgca acgatcggca tggaacccag
     3301 gatttgcagc gggacgattt cgctcagaat ccggcgcggc acggtccctc gagcggcaag
     3361 gaacatcctc acggcgtctg aacgcggacg tccggaccat tacaacagtg gcgaggctgg
     3421 gctccgctgc tgttgtcgcg ttcagatatc ggcatagcag cgtaattcga cggcgccgcc
     3481 ggggtgcgtg acggcaccgc gccgtgccgg gcccacattc tgggcaaagc gccatagcgc
     3541 gcccgaaccg aatgcggccg cctttggctt ccacacggag cggcggcgga cgagttcggc
     3601 ctcggaaagc tccacgtcga tcgtgccttt ttccgcgtcg atgacgacga tgtcgccttc
     3661 cttcagcagc cctatcggcc cgccttccgc agcctccgga ccgacatgac cgatgcaaag
     3721 cccgcgcgtc gcgccggaaa aacggccatc ggtgatgagc gcgattttgt cggacagccc
     3781 ctgcccgtac aaagccgccg tggtcgacag catctcgcgc atacccgggc ctcccttcgg
     3841 cccctcgtag cggatgacga tgacatcgcc ttccttgtag ttgcgcgcct gcacgacggc
     3901 gaaggcatcc tcttcgcagt cgaacacgcg caccggtccg cgatgatgca aatgcttcag
     3961 cccggcgact ttcacgatac atccttcggg cgcgagattg ccggacaggc ccacgacgcc
     4021 accggtcggc gcaagcggat tcgtggccgg ccgcatgacc tcttgcgccg cattgaatgt
     4081 cacgccgctg agattttccg cgatcgtttt accggtcacc gtcatgcaat cgccgttgag
     4141 atagccgcca tcgagcagtg tgcgcatcag catcggcatg ccgccagctt cccacatatc
     4201 cttggcgacg aaacgtccgc ccggcttcaa atctgcaaga tagggggtgc gcttgaagat
     4261 cgccgccaca tcgaacaggt cgaatgcgat gcctgcctca tgggccatcg cgggcaaatg
     4321 cagcgccgcg ttggtcgagc cgcccgtagc ggccacaatc gtcgcggcgt tctcgaacgc
     4381 ggcgcgcgta cagatgtcgc gcggccggat gttgtttgcg agcagcgcca tcacggtttc
     4441 accggctctc agcgcgtaat cgtcacgcga cgtgacttcg gccggaggtc ccgccgaata
     4501 aggcagcgca agaccgatcg cttccgagac gcaagccatg gtgttggcgg tgaattggcc
     4561 gccgcaggca cccgcgcccg ggcaggcgac gcattccagt tcatgcaatt cggactccgg
     4621 cattgcaccg gcggaatgct tgcccaccgc ctcgaacaca tcgaccaccg tgacgtcctt
     4681 gccgtggaat tttccgggga agatcgagcc gccataaagg aacaccgacg gcacgttcag
     4741 gcgcagcatc gccatcatca tgccgggcag actcttgtcg cagcccgcga tgccgacaag
     4801 cgcgtcgtag caatgtccgc gcaccgtcag ctcgacggaa tcggcgataa cttcgcggct
     4861 gacgagcgag gacttcatgc cctcatggcc catcgcgatg ccatcggtca ccgtgatggt
     4921 acagaattcg cgcggcgtgc cgccggcttc cttgacgccg cgcttggcgc tttgcgcctg
     4981 gcgcatcagc gcgatgttgc acggcgcggc ctcgttccag caggaggcga cacccacgaa
     5041 gggacggtgg atctcctcat ccgtaagtcc catcgcgtaa taataagaac gatgcggcgc
     5101 acgctccggc ccttcggtga catggcgcga gggcagcttg gatttgtcgt atgtcggcat
     5161 ggtgctcaac tttcacgttt cgatttcgag tgctgccaca tgagggcgag cggtggcgag
     5221 cggtttaccc ctgaagaagc cgctgctttt aggcggccct agcccctccc cccggttttg
     5281 cccatagttc acgcggaaaa tccaggaaaa gcccgattct ctgaagaaga atctcggaaa
     5341 ttttgatgcg aatagcggtg caattgggtg caatttccaa aattccggcg ctcgtgcgtt
     5401 cgcatcatat ggggttcccg gtggagatgc caaagacggc ggggctggcc gttttcttag
     5461 gttggccgtt tttcttcacc gcgattttcc gcatcggatc tccggccagg gacagcgcct
     5521 gattctgtcc gtgccgcgcg cgaaaaatag agccgcgtga cggggccagc gtttgcacag
     5581 ggcgcgcaat gtcttccgcg gatcgggatt gccgtttaac tccgtaacgt agcgtgcgca
     5641 gacttgccgc cctctccgcg ttggagaagg tgacgatgtt tgcgcatcat catgcccgct
     5701 tcggcgtgta atcgccctgc ccgctcgcga gccaggcttc gcacgcggcc ttcgccaatg
     5761 tgcgcacacg gccgatatag gccgcgcgtt cggtcaccga aatgacgccg cgcgcatcga
     5821 gcaaattgaa attgtgactc gccttcaggc actggtcata ggcaggcagc gcgagtcccg
     5881 catcgagcag gttcttgcat tccttttccg catcctcgaa atgacgcttc aaaatctcgg
     5941 catcggaatg ctcgaaatta tatttcgaat attcgatctc cgcctgatgg aacacctcgc
     6001 catagcggaa ctgctcgttg aagcgcagat cgtagacgtt ctcgaccccc tgcacataca
     6061 tcgccaagcg ttcgagacca taggtgatct cagccgagac cggcgcgcaa tcgaaaccgc
     6121 cgacctgctg gaaataggtg aactgcgtca cctccatgcc gtcgcaccag acttcccagc
     6181 cgagacccca ggcgcccaga gtcgggtttt cccaatcgtc ttccacaaag cggatgtcgt
     6241 gacgcttggg atcgatgccg agcgtcttga ggctgtcgag atagatatcc tgaacctcga
     6301 tgggcgaagg cttcaggatc acctggtact ggtaataatg ctgtagccgg ttggggttct
     6361 cgccatagcg cccgtccttt ggccggcgcg aaggctgcac ataggcggcg cgccagggct
     6421 tgggtccgag cgcgcgcaat gtcgtcgccg gatgcgacgt gcctgccccc acctccatgt
     6481 cgtaaggttg caggatcaga cagccttgcg ccgcccagta attctgaagc gtgaggatca
     6541 gaccctgaaa ggtcgcggtc gatgcgggac ttgcgggcaa agcgttggct ctcggacagg
     6601 ccggggcaga tagccgcgcc ccgccggaag cgtcaagccg gggttttctt cttctcccgc
     6661 cgattgcgca cataaatgca ttcgccaacc actgccgtca gcaggatcag cgcgttggtg
     6721 acgacgaaaa tccaactgcc gagcagccag gaatagacga cgaaagcgac ggacgccgcg
     6781 cattggccaa caaacagcca gcgcgacacg ccttgggacg tccggtcgcg ccattgggtg
     6841 tagacctgcc ggccgagcgt cagaagaaga atggccgccg ccgcccagcc gatcagatcg
     6901 gtgccgaatt tcaaacgagc ctcagttcct cgccctgacg aagaacggct tcggcggcat
     6961 tcgtgttccg tccgtcgcgt tcgtgaagga ccatcccggc cgcttcgcga aacggcgcgg
     7021 tgccgtcttt cacgatttga acgatggcgc gtttgggggc gtccccttca tggggaaaga
     7081 gcggaaacag caccgcgcca tccttgaccc tcagcagctc cgccgcccgg tcggcgcggg
     7141 tgatggcagt gacggttcct cccggtctgg tcaggccgag ggccacacgc atccattgcg
     7201 atagcgcatg ttcgccgcgc atggcgcgcg cgcgagagag attcggcgat tgctgaccgc
     7261 tggcggggtg gaacggcggg ttgaagaaaa catggtcgaa ctgtctgcca ccggaaaact
     7321 gcgccgcatc gcccggttcg aaacataccc gtccgtccag gccgttacgc gcggcattct
     7381 cgttggcaag caccaccagt cctgcgtcga tgtcgatgcc gagcacccgg acgcccggca
     7441 cacgcgccgc caggcaaagg ctcgcgatgc ccgccccggc tcccaattcc agcacctgcg
     7501 cgctcgcgtg cgccggcacg gccgcggcga gcagcacggt gtcatggccg gcgcggaagc
     7561 cgttccgggg ctgcgcggcc aaaagacgcc cgcccaggaa acggtccaat gtggcaagct
     7621 cgccgctcac gccaaatcga catcggggat cgagtccagc ggctcggctt ccgccaggat
     7681 cttcctggcg tgtgcgaagt cgtcgtcggc caccatgaga cggcgcggaa ggacgccaag
     7741 gctgccttcc atcacgctca tgtggctgtc gaacaacaag acttcgacgc cagcgtcctt
     7801 cagcacggct tgcgcaaagg acaggtagac gaaattgttg gtgcgcaaga gttctttcat
     7861 gcctgaccgc gtcccccgcc tttgccgtca cgtccgttcc ggcccgcgct tgacccccgc
     7921 tcccgcttta cctaaatggc ggggtcaagg acaggcgcgc caatccgcta tgagcacgaa
     7981 tacgcgggaa accgcttctt tgccatccag tatcaatccg gttgagcggc tggccctgct
     8041 gacgggtacg gaccttaacg gggtcgatgc ggtgatccat tcgcgcatgg gcagcgaagt
     8101 gccgctgatc ccccaactcg gccagcatct ggtggattcc gggggtaagc gtttgcgccc
     8161 gctcatcaca ttggccgcgg cacgccatta tggctatgac ggagacgcgc atgtgaagct
     8221 cgccgccgcc gtcgagttca tccataccgc gacgctcctg catgacgacg tggtggacga
     8281 aagcgcgctc cggcgcggcc ttcccgcggc gaacgtgttg tgggggaaca agcccagcat
     8341 cctggtaggc gatttcctct tcagccgcgc cttccagctg atggtcgaaa tcggctcgcc
     8401 cgcggtcctc agcgttctgg ccaatgcatc ggccgtcatc gccgaaggcg aagtgatgca
     8461 actgcgctcg gccaagaatc tcgcgatcgg cgaaaacgaa tatctgaagg tgatcaccgc
     8521 caagacggcc gccctcttcg ccgccgccgc cgaagccggc gcgatggccg cgggcgcccc
     8581 cccggcgcaa tatgaggcgc tgcgcgatta tgggcgcaac cttggcatcg cctttcagct
     8641 ggtcgacgac gcgctcgatt attcgggacg ccaggcggtg atgggcaaat ccgttggcga
     8701 cgatttccgc gaatccaaag tgacgctgcc ggtgatcctg acgctgcagc gcgcgagcga
     8761 cgaggagcgc cgcttctggc gcaagaccat cgaagtcggc gtgcaggaag aaggcgattt
     8821 gacgcgcgcc atcgaactcc tggaacgcgg cggcgcattg gccgaaacga tcgagcgcgc
     8881 ccgcctctat gcgcgttggg cgcgcgatgc gctgaatgtt tgcgcgcaga gcgagatcaa
     8941 ggcggcgctg gccgacattg ccgatttcgt ggtcgaacgc gcctattaag cagccggctg
     9001 cggagcaccg atgtccggtg gcgcgatgcg tgtcgcgcgc acccaccatt cgcgatgatc
     9061 ctgcgcgatg cgctcggccg cgccgagcgc gaattgctcc tgctcgaaaa gtccaaagca
     9121 cgttgccccg cttcccgaca tctgagccag cacgcagccc ggctcgcgat ccagcgcggc
     9181 cagcacgtcc tcgatcagcg gagcataacg ggacgccggc gcttccagat cgttcgccgc
     9241 atcggcgaga taggcgacga gatcccaaac gctttgcatc gttccaggcg gctcaatggc
     9301 gccgatgccc gtgcgcgcat tgaggccggc gaagatcgca cccgtcggca ccaggactcc
     9361 cggattgatc agaatgagcg ggaattgcgg catcggcggc atctcgcgca catgttcgcc
     9421 tcgcccctcc atccagcagg tccgcgacag aagacaggcc ggtacgtcgg agccgagcgt
     9481 cgcagcgatt tcgagcaggg cggattcggc gaggccgaga ttccagtacg cgttcaaccc
     9541 gcgcagcgcg gcggcggcat cggccgaacc gccgccgagc cctgcggcga ccggaagatt
     9601 tttttcgaga gcgatgtgcg caccccgttg agcgccagac gcgttcgcgg cgagcgcgcg
     9661 ggcggcgcgg agcaccagat tgtcgtcatt gcgcacgagc cggttcgcaa atggccccga
     9721 gacgtccagc gtcagcgtgc ttgcggcgcc aatgtccagc cggtcgccga tttgcgcgaa
     9781 cgccaccagg ctttcgagcg catgatagcc atcggcgcgt ttttcgccgg catgcaggaa
     9841 aagattgatc ttcgcgggcg cgaagagtct catgggcgcg aacgcagcgt cacgagccgg
     9901 acgcggacac cgggtcgagc ccgaactgta gcttgcgctc gatgaccggc ttctgcttga
     9961 catccggatt catctgcagc gcgtgctgcc actggaagcg cgcatcgatt ttgcggccga
    10021 cccgccaata ggcatcgccc agatgatcgt tcacatccgc ggcgcccggc gtgagctgca
    10081 cggcctcctc cagcgcgcgg gccgcatcct gatagcgtcc gaggcggtaa taggcccagc
    10141 cgaccgaatc cgcgatcatg ccgtccagcg gacgaagcgc gcgcgccttt tccagcatgg
    10201 acaccgcttc ttccaaattc tggcccttat ccacccagct atagccgaga tagttcagca
    10261 catcggcccg gtcgggattg agcttcaacg cggcgcggaa atcgcgttcc gccgcgtccc
    10321 aatgattgga gcgctcgagc gaaatgccgc gcgcgtagta aaggccgatc aaccgcctgt
    10381 cgcctgggcc gattccggcg atcgctttgt cataggccgc caccgcatcg ttatattggt
    10441 ccgcgctgcg gaaaagatcg ccgagcgccg tccagacatc ggattccttg ggctgcatcg
    10501 ccgcgagcgc tttcatcttg gcaatgcccg cgttcacctg tcccaggcgc ccgtcgttga
    10561 tcgcggcctg aacctgggtc atcccgtaat agagcgagac cggctggatc ttcgcgtaga
    10621 ggctgttggc catgtcgtat ttctgcatcg cctcgtaacg gctggcgagc agaaccttgg
    10681 cgagatcgaa gtccggccgc atgtaaagcg tgaggttgag atagaggatc gcgacatcgg
    10741 cgcttctctg gtcgttcagc gatgcggcaa tcccgaacag cgcctccgac acgccttcgg
    10801 cgggggagtt gacgagcggt tggggaatct ttttcgcggc gatatccttg agcgcccatc
    10861 cggccaccgg attgcccgga ttttcgctct gcatgcgttc atagaacgcc ttcgcctcgt
    10921 cgatgcggcc gtgtcgctgc agaaaacgtc cataggcgtc cgccgcgcgc gggcccgtac
    10981 ccatcaccga cacggcctgt ttgtagctgt cgtccgcttc cttgtcgcga ccggcaaaat
    11041 ccaggatcaa cgcgcgatgc atcagccgca gaccgtcgac accgcgctgc gccgaaagcg
    11101 tatccagcga tgcgagcgcg ccatcgatat cgtgcgaacc cgcccgcgcc caggcgtcga
    11161 tcacgcgatt ggtcagcgcc gcgaacaaac ccggcgcggg ctggtcgaca tccttgatca
    11221 cccccgcata atcttttttg acgagcgcgc cgacttcacg caccagatga gcgggacgat
    11281 tgtccgtatc cagcgccagc acccgatcgg tcaaaccgat ggcctgatcg atatcgccgg
    11341 cttcggcggc aaacaggaac gtgcgctcca gaagatcggc gttgccggga tcgtctttca
    11401 acgaatgcgc atagaagccc gtcgccgctt tgacatcgcc ctgggcggcc gcgaaattgg
    11461 cggcgagata ggtgcccgcg agcgagttct gcgacgcctc gcccgcgacc ggcgcggagg
    11521 cgctcatcat cgagcctgct gtgctgcatg cgccgagcga cagcgcggtc aaactggacg
    11581 ccagagccaa tagtgttttg agctgcacgg gcttatgcct tcctctcgac ttgcgaaatc
    11641 atagccggga ttgaacgttc cgtcacatgg tgccgtaggc cggaccatcg cctccttcgg
    11701 gcgcacacca tataatattc tgcgccgggt ctttgatgtc acaggttttg caatgaacgc
    11761 agttcgccgc gttgatcaca aagcgcgggc cgctgtcgcc cgtcaccacc tcgaacaccc
    11821 cggccgggca gtagcgctgc gccggctccg catagacggg caaattcacc gcaatgggta
    11881 gagcgggatc cttcaatttc aggtgaacgg gctcatcttc gtcgtgccgt gtcccggaga
    11941 ggaaaaccga agacaatttg tcgaaactga cggaaccgtc aggctttgga taggcgatcg
    12001 gccgggcatc cttggccgga atgagctgcg cataatccgg acctttgccg cgaagcgtgc
    12061 cgaacgggct gaagccgaac aattgattga accacatatc gagtgcgccc acgggaacgc
    12121 ccaataccgt cccatagcgg gaccacagcg gttttacgtt ccgcacacgc ttcaaatcct
    12181 tgcagacgga agaaatgcgg taggcatcct catattgggt gagtctatcg cccgcccgtt
    12241 cttgcgctat ggcgtcaaag gcggcatcgg ccgccatcat tcccgtgagg atcgcgttgt
    12301 ggctgccctt gatgcgcggc acattgacga agccggcggc gcaaccgatc agagcgccgc
    12361 ctgggaaggc gagtttcggt acggcctgaa agccgccctc ggtaatcgcg cgcgcgccat
    12421 aggcgatgcg gcgtccgcct tccagcatcg gccggatgaa cggatgggtc ttgaagcgct
    12481 ggaattcgtc gaagggcgag agatagggat tgcggtaatc cagatggacg acgaaaccca
    12541 ccgccgcgag attgtcgcca taatgataga ggaacgatcc gccgccggtg tggttgtcga
    12601 gtggccatcc catgctgtgc atgacaagtc cgggccggtg cttcgccttg ggcagctccc
    12661 ataattcctt cagtcccagc ccataggttt gcggcgcgct gtctttggcc aagccgtagc
    12721 gggcgatgat ctgcttggcg aggctgccgc gcgcgccttc cgcgatcagc gtatattttc
    12781 cgcgcaacgc catgcctggc gtgtaattgg gcttgtgatg gccatcgcgc gccacgccga
    12841 gatcgccggt gatgacgccg gcgacttcgc cactctcacc cagttccaca tcgacggccg
    12901 cgaacccggg ataaatctcg actcccagcg cttcggcttg ttccgcaagc cagcggcaaa
    12961 ggagaccgag gctgacgatg taattgccgc gattgctcat cagcggcggc atcaggagat
    13021 gcggcaggcg gatatggccc gccggtccga gaaaatagaa acggtcctcg gtgacttgcg
    13081 tctccaccgg cgcgccgcgc gctttccaat cgggaatgag cgcattcagt ccgatgggat
    13141 cgacgatggc tccggaaagg atatgcgcgc cggcctcggc gcctttctcg atcacgcaga
    13201 cgccgatctc gcggccctcg cgctgggcgc gctgcttcaa acggattgca gccgcgagac
    13261 cggcaggacc cgcgccgacg atcacaacat cgaattccat gctctcgcgc gcagcgctca
    13321 tcgcccggtc ttaagcgggg ggcatttgtc cggtcaatgg caccgaagga agactgacgc
    13381 ccttggcgca aatcgcgtac attcggccca tggccgatcc cacccgcgaa cgatcacttg
    13441 ccgcgctgcg ctggctcatg gaagccggcg cggacgaggc gattgccgag gagccggtga
    13501 accggttcgc acagcgcgcc cgtattaccc cggatgccga ggacgccgta tcggcacgca
    13561 gcggcgaacg gcctggcggg acaagcctta tgcgcgctcc cctcgctcaa agtgagggta
    13621 tgaaacgcac tgccagtgcg gaagcggtct ctctgtccac ggcgccaggc gcggcacgcg
    13681 cactggccgc ctcctgcatg aacctcgatg cgctgagggc tgcgctactg aacttcgatg
    13741 gctgcgaatt gaaacgccac gccaagaaca cggtttttgc cgacggaaac cctttgggcc
    13801 gcatcatgct gatcggcgag gcacccgggc gcgatgaaga cgaacagggc ctgcccttcg
    13861 tgggacgcgc cggaaagctg ctcgaccgga tgctctcggc aatcggcctc gaccggagca
    13921 aggtctacat caccaatgtg cttaattggc gccccccgca aaaccgcgat ccctcgcccg
    13981 atgaagccgc ggcctgcctt cccttcctgc atcgccatat cgagcttgcc gagccccaga
    14041 ttttgatcct cctgggggcc gttgccgtgc gccatgtgct tggactcacc gagggcatca
    14101 tgcgcgcgcg cgggaggtgg caactctacc aaagcgtgca tctgggccgg gcgatccccg
    14161 tcatgccgac cttgcacccc gcgtttctcc tccgtcagcc cgttgctaag cgcttggctt
    14221 ggcgtgatct tttagagatt tcggaaagaa tcgaacagct tgggcttgct tgattaatct
    14281 ctggacggca ccatggtggc tgcctggagg gcccgatttg aaaaaattcg ccgcgatcgc
    14341 acttgccggt ttctgttgca tcgccgcttt cgcgagcgct cgggccgccg accaggtcgc
    14401 cagttccacg attctgtcgc ccgccgacgt taagacctat ggcgagattt tcgcggccca
    14461 gaaaaccggc caattcgcga aggccgaccg gctcatcgcc aaactcgaag acacatcgct
    14521 gctagggtac gtgctccagg atcgctatct cggcgcgcac tatcggtcca aattcggcga
    14581 actcaaatcc tggctgacag aatatggcga tctcgtaggc gccgacggca tttacaaact
    14641 cgccgtcaag cgggcgccga aaaagaccgc cgttccggtt ccggcgcgcg cgcattggcg
    14701 cggggccgcg ggcgatggcc tggcgttcga caacatgaaa ttgcaatcgg aggccgccga
    14761 acgtatcgcg cagcaattgc gcagccttgc ccgcgacgac aggcccgaag acgccgacgc
    14821 gctcgtgaaa aaactcgcgg ccacccgttc ggggttgcag cagggcgaca tcgacaggct
    14881 cgcggcctat gtcgccaccg catatcttgg cgaaatgcag gatcaggccg cgctttcgcg
    14941 cgccgaggaa atcatcgccc gtcacggcat cagcgccgtc caatgcgaat ggacagcggg
    15001 tttggccgct taccggcttg gccgcttcga agacgccgcc caccatttcg aagccgtgat
    15061 gcaaagcgcc agcgctccgc gcacctatgc ggcggcggcc ttctgggcgg ccagaagctg
    15121 gaccggcacc ggtgaggccg aacgcgtgct gaccctttat gagcgcgcgg cgagcgaagg
    15181 cgccgacacg ttctacggcc ttctggcaac gcggctgctg ggccgtgaca tgaaagccga
    15241 tttctccgag cccgcgctcg attcggtttc ctttgccggt ctgatgctgg aaaagcccgc
    15301 gcaccgcgcc gtcgcgcttt ggcagatcgg ccatgccgaa gcggtggaag cggaactcgc
    15361 gcgtgcgttc ggccagatgt cgcccgatct ggatccggct ttcgccgccc tggcgcgcag
    15421 cctgggcgcg ccggcgctcg agcttcgcgc cgccgagacg gccgctccca ccgtacggct
    15481 caccagcctt tatcccgtgc ccccttatga gccccaaggc ggctatgcgc tcgatcaagc
    15541 ggtcgtgctc gccttcgcgc ggcaggaaag ccgattcgaa cccggcgcat tatcgaaggc
    15601 cggcgcccgc ggcgtgatgc agatcatgcc ccagaccgcc gtgaccatca cccatgattc
    15661 atcgctcgcg ggccgcaaca aggaccggct ggacgatccc acctacagca tcacgctggg
    15721 ccagaactat ctgctcgatc tcatcgaccg gcagaacggc aatcttttca gcatcgcggc
    15781 ggcctacaat gccggcgagg gcaacctgac gaagtggcaa tcgcaacgcg ccgacgacag
    15841 cgatccgctt cttttcatcg agaccatccc gctcgccgaa acgcgcgact acgtcaagcg
    15901 cgtgatgacc aatgtgtgga tgtaccgctt gcgtctgggc gaggctccgg acgggctcga
    15961 cgcggcggcc gccggctcat ggccgactta tacccaggca gtccaggccg gcaccgcttc
    16021 cgtctctcca tagcggaaca tggctgaagt ccggccgcgc gaattcatcc ctgtacggat
    16081 cgcagtgctg acggtctccg atacgcgcac ccgcgccgac gatgtttccg gcgacacgct
    16141 ggaggcgcgt gtgcgcgagg ccggccatca tgtcgccggc cgggcgattg tgccggacga
    16201 gatcgcgcgc atcgccgaac agcttatcgt ctggatcgcc gatcccgaga tcgacgtcgt
    16261 catctcgacc ggcggcaccg gacttacggg ccgcgatgtc accccagagg cgtttcgcag
    16321 cgtgttcgaa aaggagatcg atggtttccc ggccgtgttc caccgcatca gctacgacaa
    16381 gatcggaaca tcgacgatcc aatcgcgcgc gtgcgcaggc gtggcgaaag gaacctatct
    16441 cttcgcgctg cccggttcgc cgggcgccgt gaaggacggt tgggacggca tcctgaaggc
    16501 gcagctcgat tatcgccacc gcccgtgcaa tttcatcgaa atcatgccgc ggctggaaga
    16561 acatttgcgc cgcgcgaaat agcttaatga attcgcgcgc cgtcctcggt gaggatggac
    16621 cagaatgccg ggcgctcatc cctcacggcc ttcagcacat cggcgagcac gcaatacgcg
    16681 ggctgacgca agccatgata ttcgaccagg ggaccgatgg gccgaaagcg aatcgccatg
    16741 gcggagagca tccgcaacag cgtcggttcc atcgcggcgc accaatgggt gatgccgtgc
    16801 tctctgctca tgcgcaccag cgcctggatg agacccaacc gcatcaaagg tcccgaactg
    16861 ccaagcaagt cgatgtctgc cccaccattg gacagcccgg cgatgcggcg gaattgacga
    16921 gagatgctga agcgcgacac ctcgcccgtc gtctgaagcg gaaacttttg aaattcgcta
    16981 agcgcggcgg cgtccatcac acgctggacg gcgaagctgc ggtgcaacgc atcgggcctg
    17041 ggcaatatca gacgcacggt cccaagtggc gtcttggcag cccggtggat cagaaggctt
    17101 tgggcggcat gagaatcgaa ttcatccatt tcctcgccgt cgggatcgtc cgctttctcg
    17161 tgcgagtgtt cgacacaata gacttggtag cgtattcgct gcgcgatccg aacgcgttcc
    17221 agcgtgttgg cgggaagcac gtcgaattgc gattggaatc gttcaagcag cgtgccggct
    17281 cctgttgtgg aatcggcatt gcctgcaaga ggcaggttat tttccgctgt gtcagctatc
    17341 tccgcgaatt gcaggatgtt gtgcaatgtc atctttgagt cccccatagc cgcggccacg
    17401 ttcgcgtgac caatccatgg ctgcgtaagg tcctcccggc cgattcctgc cgcttaaaac
    17461 ccccgcagcc tctctcaggc taccccaacc ggccgtgctg tttgcctccg tcctttcttt
    17521 gtgccgacag cctccgtccc ttctttgtac agatggatta cgtgcacgga tttgagcggc
    17581 aattttgtga aattttccgc tgtcgccgtc gtcgcggcaa cgcgaacgat acagggaaga
    17641 tgcgcacaag atacagaaaa tcgcgcgaat accgccggga cgcgcgcgaa atcgcaggcg
    17701 ctacacgctc aagcggaatc gaagacgcaa agattgcgtc acacgggaat gccaaacatg
    17761 gttaataacg cacggcggta tattccacgc caggagatcg tcaatgctgc ccgcgcaccg
    17821 caaagatgtt caaccacgct gagaaataac ttgcgaccga ggtagcgccg gtcttgcgca
    17881 ccacctcttc ccaacgcgcc gcatcgcgtt ccagcgccgt ggcatagctc aacagatcgg
    17941 aaaccgttgc gcgatccttg aaggccgccg caagatagcg cgcctcgctc gcctgtttcc
    18001 gcagctcggc ggtatcgacc aaggtgtcag acttctgcaa ggatttttct ccctcaactc
    18061 cgtaagtata gcacggaaaa ggcataaagt tccattgccg cgccggagca ggacgttcgg
    18121 attgcgaatc agcgctcctg cggcgcgccc ggggctcgac cttgctgagc cgggccttgc
    18181 tgcggcgggc caccatccgg ggtcacggga accgcctccc agcgtccctc gcagttccgc
    18241 acatagggat aaaacccgtc cggattcgag caatgatacc agctctgctc gggcggaggg
    18301 gcatccggtg gaggaagata atcgggcggc ggcgcgtcat aatcacctcc gtcttgcaca
    18361 acgaccgg
//
LOCUS       DASYEG010000336         3907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_280058, whole genome shotgun sequence.
ACCESSION   DASYEG010000336 DASYEG010000000
VERSION     DASYEG010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280058"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1745)
                     /locus_tag="VGM72_14105"
     CDS             complement(72..1745)
                     /locus_tag="VGM72_14105"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEY4266446.1"
                     /translation="MTASVASPSMSPPDPGVAAAKNAAAAPDSDTGFGAMLSQLAGTP
                     DATAPASANAPAAEDHGSAQSDAAATAAAAANPMPWMAQLEAAKILPPVQDAADEPDA
                     PPTGNPPQQNQAQGTATQSPASQPSVPVAAPLPALPDSATDEAGTALTAAASDAVPET
                     ADAQANATGASAAVEQPATPDRPKEQKSPHAVADSDQNLPQDAAALTDLSPPVAAQLL
                     GVPAPPAAQSAPTPQSPPDVTATPIPKPEETLAASSLQAAAQAALAPMDKSVTPKNAS
                     ASETKPSAEARSSGPRDARAAASADSTAAPANTNDAPERNVDNTPANAPVNAAGNAPS
                     TAPGNAPGTAPANAPAPTGHTTKDEPAGAAANLSEPAPAINPLANHAAFEIPAAAAAN
                     APPAAADAPVKLSFTAPNAANVPSFDALALKIAARSSDGDNNFSIRLDPLDLGRIEVN
                     LNVRSDGHARAELSADKPQTLELLQKDAPALERALKDAGLNLAGGLAFSLKGDGRSQT
                     WRDPQSGSRGRALQIAAVDAANANADVAVSTALAAHAYGLPKARLDIRI"
     gene            2058..>3907
                     /gene="flgK"
                     /locus_tag="VGM72_14110"
     CDS             2058..>3907
                     /gene="flgK"
                     /locus_tag="VGM72_14110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HEY4266447.1"
                     /translation="MALNGIISSGLTAIQTNSAALRVTSDNIANINTPGYVRRVAQQQ
                     TLAPGGVLSGVQLSSVQRMADEYLDKEVLSASGNSARYDVQSSIMDQLNAALGAPGDG
                     NSIGSQLDAVYASLGQASLDPSSITNRLGSLGQFNTLADSISGLAGSVADLRTNTDQQ
                     VSAVVSQANTLIKQISDLNPQIQHAVISGDTASGLLDQRDSLVQQLSQVMGITTTTQS
                     DGRIFIATSDGMQLVSDNYAVLAYHPSSGSSFNPITVQTVNAISGQTIGPSRDFDSHA
                     TSGQLRGLLDMRDGTLADIGKELGSLAQSVSLAFNAAHNANAAVPPPTTLDGRQTGLL
                     STDALNFSGATTIGVTDSSGTLLHKIAVDFDAGTLSVDGGPATTIGGTVGSFTTALNT
                     ALGANGSASFTDGALTLSATGTNGVVIADDAANPTSRGGTGFSQFFGLNDLFQSTGNA
                     IVTTGLTSSDTAGFAPGGSISLLLKGPQGQRVGETSIAVTGTTIGDVVTSLNSAFTGK
                     ATFTLDSNGQLQVTPAAGYSGYSLEVTQDSTQRGTTGESFSTLFGLGGAQQMALAQGF
                     SLAPDLVNSPQRLAFAKPSLDSTTALATNVVASGDNRGLLALQDLMNQNQS"
BASE COUNT          649 a         1228 c         1304 g          726 t
ORIGIN      
        1 gcggcgtcgg acgctgtgac cggtgtcgtc gggggcgccg ctgtggtcga tgttacagac
       61 atgtcctgct cctaaatcct gatgtccaaa cgcgccttgg gaagaccata ggcatgagcg
      121 gcaagtgccg tgctcacggc gacgtcggca tttgcgtttg ccgcatcgac ggcggcgatc
      181 tgaagcgcac ggccgcgtga accgctctgc gggtcgcgcc aggtttggga cctgccgtcg
      241 cccttcaggg agaaggcgag gccgccggcg agattgagac ccgcatcttt cagagcgcgt
      301 tcgagcgcgg gggcgtcctt ttgcagaagt tcgagcgtct gcggtttatc ggcgctgagt
      361 tcggcccgcg catgaccatc ggaacggaca ttgagattca cctcgatgcg gccaaggtcg
      421 agcggatcga gacgaataga gaaattgttg tcgccgtccg aggaacgtgc cgcgattttg
      481 agggcaagcg catcgaagga tggaacattg gcagcgttcg gtgcggtgaa gctgagtttc
      541 accggtgcat ctgctgcggc aggtggcgca ttcgcggcag ccgcggcagg gatttcgaag
      601 gccgcgtggt tcgcaagcgg gttgatcgcc ggcgctggtt ccgaaaggtt tgccgcagca
      661 ccggccggtt cgtctttcgt ggtgtggccg gtgggtgccg gggcgttggc cggggcggtg
      721 cctggggcat tgcccggggc ggtgcttggg gcattgcccg cggcgttgac tggggcgttc
      781 gccggggtgt tgtcgacgtt acgctcgggc gcatcgttcg tgttggccgg ggccgccgtg
      841 gaatcggcgg aggcggcggc gcgggcgtcg cgcgggccgg aagatcgtgc ctcggcggac
      901 ggttttgtct cggatgctga agcgttcttc ggggtgacgc ttttatccat aggcgcgagc
      961 gccgcctggg ctgcggcctg cagcgaagac gccgcaagcg tctcctcggg tttgggtatg
     1021 ggggtggccg tcacgtcggg cggcgactgc ggggtaggcg ccgattgcgc ggcgggcggt
     1081 gccggtacgc caagcaattg cgccgcgacg ggcggcgaaa gatcggtaag cgccgccgca
     1141 tcttgcggga ggttttgatc gctgtctgcg accgcatgcg gggacttttg ttccttggga
     1201 cgatcgggcg ttgcaggctg ttcaaccgcc gcggatgcac cggtcgcatt ggcttgcgcg
     1261 tcagccgttt cgggaaccgc atccgaggcg gcagcggtca acgcggtgcc ggcttcgtcc
     1321 gtcgccgaat cgggaagggc gggtagtggc gcagccaccg gaacggacgg ttgggatgcg
     1381 gggctttgtg ttgcggtgcc ttgtgcctgg ttctgctgcg gtggattgcc ggtgggcggc
     1441 gcatcgggtt cgtctgccgc gtcctgcacc ggcggcagga ttttcgcggc ctcgagctgt
     1501 gccatccatg gcatcgggtt ggcggctgcc gctgccgtgg cggctgcatc ggattgcgcg
     1561 ctgccatgat cctcggcggc gggcgcattg gcactcgcag gcgctgtcgc gtccggcgtt
     1621 cccgcaagct gcgacagcat cgcaccgaag cccgtatcgg aatctggtgc tgcggcagcg
     1681 ttcttcgcgg ccgcgactcc aggatccgga gggctcatcg aaggcgaagc gacagacgca
     1741 gtcatgcaaa ctccggcact gatccgcagc ctccatagca aggaccggac cagcgaaaat
     1801 cagagccgcc gtctcgcgcg cgcaagctat agagggaatg gaaaaccaac cggcccacga
     1861 cgcgtgcgcc cgtcccgggc acggcgcggt tcggacgcgt tcccacccgg catgaaatgc
     1921 cccatacggc aaagcctacc cgttccggtg cgtcgcaaac ctatgctttg gcgtcatttg
     1981 cggcctgccg ggatggcacc ctccttgcac atgacggccc gaacctatga ctcaaagcct
     2041 ggagcaacgg cttttcgatg gcactcaatg gcatcatttc cagcggtctg acggcgatcc
     2101 agacgaacag cgctgccttg cgcgtgacgt cggacaatat cgccaacatc aacacgcccg
     2161 gttatgtgcg ccgggtggcg cagcagcaga cgcttgcccc gggcggggtt ctttccggcg
     2221 tgcagctttc cagcgtgcag cgcatggccg acgagtatct ggacaaggaa gtgctgagcg
     2281 cgagcggcaa ttcggcgcgc tacgatgtgc aatcttcgat catggaccag ttgaacgcgg
     2341 cactgggcgc gcccggagac ggcaactcca tcggttcgca gctggatgcg gtctacgcct
     2401 cgctcggcca ggcctcgctc gatccaagct cgatcaccaa caggctcggc tcgctcgggc
     2461 aattcaacac gctggcggat tcgatttcgg gcctcgccgg ttcggtggcc gatctgcgta
     2521 ccaacaccga tcagcaagtc tccgctgtcg tttcgcaggc caacacgctg atcaagcaga
     2581 tctcggacct taatccgcag attcagcatg cggtcatctc tggggatacc gccagcggcc
     2641 ttctggatca gcgcgattcc ctggtgcagc agctttccca agtgatgggg atcaccacca
     2701 cgacgcagtc ggacggacgc atcttcatcg ccacgtccga tggcatgcag ctcgtcagcg
     2761 acaattatgc ggtcctcgcc tatcatccga gttcaggctc gtccttcaat cccatcaccg
     2821 tgcagaccgt gaacgcgata agcgggcaaa ccatcggacc gtcgcgggac ttcgattcgc
     2881 acgccacgag cggacaattg cgcggcttgc tcgatatgcg cgacggcacg cttgccgata
     2941 tcggcaagga gctgggctcg ctggcgcaat cggtgtcgct cgccttcaac gccgcgcaca
     3001 acgccaatgc ggcagtgcct ccgcccacca cgctcgatgg ccgtcagacc gggcttctgt
     3061 ccaccgatgc cctcaatttc agcggcgcga ccacgatcgg cgtcaccgat tccagcggta
     3121 cgctgctgca caagatcgcc gtcgatttcg acgccggaac gctcagcgtc gatggcggcc
     3181 cggccaccac gatcggtgga acggtcggca gtttcacgac ggcgctcaat acggcgcttg
     3241 gcgcgaacgg ctctgccagc ttcaccgatg gcgcgctgac gctgtcggcc accggcacta
     3301 acggcgtggt gatcgccgac gacgcggcca atcccacatc gcgcggcggc accgggtttt
     3361 cgcaattctt cgggctcaac gacctgttcc agagcaccgg aaacgccatt gtcacgaccg
     3421 ggctcacttc gtcggatacg gccggctttg cccccggagg aagcatctcg ctgctgctga
     3481 aaggtccgca gggccagcgc gtcggcgaaa ccagcatcgc agtcaccggt acgacaatcg
     3541 gcgatgtggt gacgtcgctc aattcggctt tcaccggcaa agcgacattc acgctcgatt
     3601 ccaacggcca attgcaggtc acaccggccg ccggctacag cggctatagt ctggaagtca
     3661 cgcaggactc cacacagcgc ggcacgaccg gggaaagctt cagcacgctc ttcggcctgg
     3721 gcggcgccca gcagatggcc ctcgcacagg gtttcagtct tgcacccgat ctcgtcaatt
     3781 cgccgcagcg tctcgccttt gccaagccgt cattggattc cacgaccgcc ctggcgacaa
     3841 acgtcgtcgc ctcgggcgac aatcgcggtc ttctggcgct gcaggatctg atgaatcaga
     3901 accaatc
//
LOCUS       DASYEG010000337         6399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3325263, whole genome shotgun sequence.
ACCESSION   DASYEG010000337 DASYEG010000000
VERSION     DASYEG010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6399
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3325263"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(311..1255)
                     /locus_tag="VGM72_14115"
     CDS             complement(311..1255)
                     /locus_tag="VGM72_14115"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4266448.1"
                     /translation="MPLDPTIQALFQQMPQLTNYPIWEKTPAEARAAFKALCQFANPK
                     AAAIGKTEDISAPGPAGPVPLRIYTPVAAGTTALPGIVYFHGGGFVLGDLDCYDGLCR
                     ALANESGCRVVSVEYRLAPEHPFPAAVEDCFAAAKWVEQHAPDLGIDPNRLAIAGDSA
                     GANLAAVTCLLAKANKLTPHIAFQLLIYPVTHFERAASIRPFGSGYFLENRTIDWYRD
                     LYVPGDADASDPRLSPLKAKDVSGLPPAYIVTAGFDPLRDEAIAYADRLKQAGVTVNH
                     VDYPSMIHGFFSMQGFIPLSIEAIGAAAHAVRDALNDA"
     gene            complement(1411..1617)
                     /locus_tag="VGM72_14120"
     CDS             complement(1411..1617)
                     /locus_tag="VGM72_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266449.1"
                     /translation="MSAEETCRLAAELQEEFGDLVVVVARRTIASLEADGLEERASIW
                     RALLAILDDIAEKRIDPYAKIAIH"
     gene            complement(1651..2199)
                     /locus_tag="VGM72_14125"
     CDS             complement(1651..2199)
                     /locus_tag="VGM72_14125"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEY4266450.1"
                     /translation="MTGPREILDFWFKETPPERWFMADPAFDAMVRNRFEEIWRAAST
                     GAFASWADTLEGALALIVMLDQFPRNMFRGSSEAFSTDGMARIAARNAIARGFDVEAP
                     LGMRSFFYLPLMHSEDLRDQEECVRLTRARLGENHFSYPYALRHRDVIAQFERFPARN
                     VPLARDATAEEIEFLQANPNGF"
     gene            complement(2220..2651)
                     /locus_tag="VGM72_14130"
     CDS             complement(2220..2651)
                     /locus_tag="VGM72_14130"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEY4266451.1"
                     /translation="MGRLIGIARAVKKRARLAELEETDVRLADGIIGDVRGATRDRQV
                     TVLFREGWNAACDALGVQLPWVTRRANLLVEGVPVPNESERLAIGDLILEVTGETKPC
                     SVMEAAHRGLRRAMAPQWRGGVCCRVVSGGHIHIGDFVEIV"
     gene            2745..3968
                     /gene="coaBC"
                     /locus_tag="VGM72_14135"
     CDS             2745..3968
                     /gene="coaBC"
                     /locus_tag="VGM72_14135"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEY4266452.1"
                     /translation="MSLNLDGKRVLLIIGGGIAAYKSLELIRRLRERGAGVGAILTEA
                     GSHFVTPLSVSSLTGEPIHRELFDLTAESEMGHIALSRSADCVVVAPATADLIAKLAA
                     GLAPDLASTALLATDKPVLIVPAMNVRMWEHAATRRNLITLRRDGIVIIGPNEGEMAC
                     GEFGPGRMAEPGEILAAIERQLTPDTRLAGLKALVTAGPTQEPIDPVRYIANRSSGKQ
                     GYAIAAALAEAGAQTVLISGPVEIATPPGVKLVKVQTAREMWSACQSELPADIAVCAA
                     AVADWRPETIANAKLKKRNTAPALPLVENPDILAGLSSHASMRPKLVIGFAAETDDLI
                     ANAAAKRVAKGSDWIVANDVGAKESGESVMGGDSNRVHLLTASGTEAWPQMSKTEVSR
                     RLADRIASAIGRNAA"
     gene            3965..4411
                     /gene="dut"
                     /locus_tag="VGM72_14140"
     CDS             3965..4411
                     /gene="dut"
                     /locus_tag="VGM72_14140"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEY4266453.1"
                     /translation="MKLAIQRLAHGQDLPLPSYATAGSAGLDLFAAVAREFVLMPGRY
                     AAIPTGIAIALPDGFEAQLRPRSGLALRHGITVLNAPGTIDSDYRGEVTAILINHGVE
                     PFAIPRGMKIAQLVIAAHERIEWSETTMLESTQRGTDGFGSTGLGV"
     gene            4417..4875
                     /locus_tag="VGM72_14145"
     CDS             4417..4875
                     /locus_tag="VGM72_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY4266454.1"
                     /translation="MFRLSRKTMLALEAVIDIAYNARPEPVQAKEITARQGVPQRYLE
                     QVMQQLVRAGILKGVRGPRGGYRLARERRRISVGDVVRIAMSLDENEENFDGGSELGR
                     RVVVPLVASLQEEVMKKLDEITIEDICKRARVSGLDGGVTERPDTADFII"
     gene            4899..5888
                     /gene="cysK"
                     /locus_tag="VGM72_14150"
     CDS             4899..5888
                     /gene="cysK"
                     /locus_tag="VGM72_14150"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEY4266455.1"
                     /translation="MADTIPKDKRGPRHKPGRGRIYNSITDTIGDTPLVRMVRMPQAA
                     GVKADLLFKLEFFNPLASVKDRIGVSMIDALEREGKIAPGKTVLVEPTSGNTGIALAF
                     VAAARGYHLILVMPESMSLERRKMLTLLGAQIELTESAKGMKGALARADQILATTPRS
                     IMPQQFENPANPDIHRRTTAEEIWNDTEGKVDVVISGVGTAGTITGVGQVLKARKPSV
                     KMIAVEPEDSPVLSGGQPGPHKIQGLGAGFIPEILDRSIIDEVVTISNETAFETARAA
                     ARLEGIPGGISSGAAIAAALEVGARASMAGKQIVTIIPSFAERYLSTALFEGL"
     gene            complement(5885..>6399)
                     /locus_tag="VGM72_14155"
     CDS             complement(5885..>6399)
                     /locus_tag="VGM72_14155"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEY4266456.1"
                     /translation="HSKMTPRDGERMPSLSEVIEAVRAVKEDFRLFIEIKTSIGDRTL
                     SQSAEAVAEAVVGELRAAAFREFAVLVGFDWKGLIHAKMLDPELPCWFSTERKSRLGA
                     DAILAHGGDGWFCALERTNTEAVQAARACGLSIGIWTVNDVRELRALLGLGVDALCTD
                     RPDRLKILSR"
BASE COUNT         1250 a         2020 c         1990 g         1139 t
ORIGIN      
        1 agcgagccga ccagcgtcac ttccaggccg tccagaatcc aggtgatgcc cagcgcaacc
       61 accaccagcc aatgaaagcg gccccatcga aggcggtcga gccgtgcggg tacgtcgctc
      121 aggaaagtgt cgtcgccgag agccgccatg agatgccgcc gataacgcgc gggcggggcc
      181 aagtccaggg cgacgggtca acagagagaa ctacagggcg ccctcagaag ggatgaaccc
      241 gaagaacacg aagaacgcac caatttgtgg ttttcttcgt gcgctttggg ttgagtcttg
      301 gttctttgcg ttaagcgtcg ttcaatgcat cgcgtacggc atgtgcggct gccccaatcg
      361 cttcgatgct gagggggata aatccctgca tcgaaaagaa cccatgaatc atcgacgggt
      421 aatcgacatg attgaccgta acgcccgcct gtttcagccg gtccgcataa gcgatggcct
      481 cgtcgcgcaa gggatcgaat cccgctgtga cgatataggc gggcggcaga ccgctgacat
      541 ccttggcctt tagcggcgag aggcgcggat cggaagcgtc ggcgtcgcca ggcacataga
      601 gatcgcggta ccagtcgatt gtgcgatttt cgaggaaata gccggagccg aacgggcgta
      661 tcgaggccgc acgttcgaaa tgtgtcacgg ggtagatcag aagctgaaac gcgatgtgcg
      721 gcgtcaattt gttcgccttg gcgagaaggc aggtcaccgc cgcgaggttt gcgccggcgg
      781 agtccccggc gatggcaagg cggtttgggt cgataccgag atcgggtgcg tgctgctcca
      841 cccatttcgc cgcggcgaag caatcttcca cggcggcggg gaatggatgt tccggcgcca
      901 gccgatattc gacggaaacc acccggcagc cgctttcatt ggcaagcgcg cggcagagcc
      961 cgtcatagca atccagatcg ccgagcacga acccgccgcc gtggaaataa acaatccccg
     1021 gcagcgccgt ggttcctgct gcaaccggag tatagatgcg aagtggaacc ggccctgcgg
     1081 gtcccggcgc agagatgtcc tcggtcttcc cgattgcggc tgccttgggg ttggcgaatt
     1141 ggcacagcgc cttgaacgcc gcgcgcgcct ccgcgggcgt cttctcccat atgggataat
     1201 tcgtgagttg cggcatttgc tggaacaggg cttggatcgt gggatcgagc ggcatgcggg
     1261 cctttttcca aattggcgcg ccgccgttcg ggatgatgca gcgcacaaat gcatggtctc
     1321 agggacaata gcgcaattcc gcccatccgg tgtgggctgg acgctcattt tgcgcggggg
     1381 aattcggcca tccccttaga ctggcgaaaa ttaatgaatc gcgatcttcg cgtacgggtc
     1441 gatgcgcttt tcggcgatgt cgtcgagaat cgcgaggagc gcgcgccaga tggatgcacg
     1501 ttcctcaagc ccgtctgctt ccaggctggc gatcgtacgt cttgccacca cgacaacgag
     1561 atcgccaaat tcctcctgga gttccgctgc cagacggcag gtttcctcgg cgctcatata
     1621 tttctccggc tttagaagcc atcacaacga tcaaaacccg tttgggttcg cctgcagaaa
     1681 ctcaatttct tcggcggttg cgtcgcgcgc gagcggcaca ttgcgggccg gaaaccgctc
     1741 aaactgggca atcacatcgc gatgacgcag cgcataggga taggaaaaat gattttcacc
     1801 aagtcgtgcg cgcgtgaggc gaacgcattc ctcctgatcg cgcaaatcct ccgaatgcat
     1861 cagtggaaga tagaagaaac tgcgcatgcc gagaggcgcc tccacatcga agccccgcgc
     1921 gatggcgtta cgcgccgcaa ttctcgccat cccatcggtg gagaatgcct cggacgaacc
     1981 acggaacatg ttgcgcggga attggtcgag catgacgatc agcgcgagcg cgccttccag
     2041 ggtgtccgcc caggatgcga acgcgcccgt gctggccgca cgccaaatct cctcgaaacg
     2101 gtttcgcacc atcgcatcga acgcgggatc ggccatgaac cagcgttcgg gcggcgtctc
     2161 tttgaaccag aaatccagga tttcgcgcgg cccagtcaaa atctatacga cctccaagct
     2221 catacgatct ccacgaaatc gccaatgtgg atatgcccgc cgctcaccac gcgacagcac
     2281 accccacccc gccattgcgg tgccatcgcg cgccgcaagc cccgatgcgc cgcttccatg
     2341 accgagcagg gtttggtctc gcccgtgacc tccaggatca aatccccgat ggccaggcgc
     2401 tcgctctcgt tcggtacggg caccccctcg accaggagat tggccctcct tgtcacccag
     2461 ggcagttgaa caccaagagc atcgcaggcg gcgttccacc cctcacggaa cagcaccgtg
     2521 acctgccggt cccgcgttgc accgcgaaca tcgccgataa tgccgtcggc gagccggacg
     2581 tcagtttctt ccagctcggc caggcgcgcc cgcttcttca cggcgcgcgc aattccaatc
     2641 aatcggccca tttctacatt ccaacgctac gtgtaagaga gaactacact tctgcttcgt
     2701 aattcgctat gtatacccct tgtttcatgg gttatcacgg agtcgtgagc ttgaacctcg
     2761 acggcaaacg cgtcctgctt atcattggcg gcggcatcgc ggcttacaag agtctggaac
     2821 tgatccggcg cttgcgggag cgcggtgccg gcgtcggtgc gatcctgacc gaggcaggca
     2881 gccatttcgt gacgcccctt tcggtgtcgt cgctgaccgg cgagcccatc catcgggaat
     2941 tgttcgatct gaccgcggaa tcggagatgg ggcacatcgc gctttcgcgt tcggccgatt
     3001 gcgtggtcgt cgctccggcg acggccgatc tgatcgcaaa gctcgccgcc ggtcttgctc
     3061 ccgacctcgc ctcgacggcg ctgctggcca ccgacaagcc ggtcctgatc gtgcccgcga
     3121 tgaatgtccg gatgtgggaa cacgccgcga cgcggcgcaa cctgataacg ctccggcgcg
     3181 acggcatcgt catcatcggt cccaatgagg gcgagatggc ctgcggcgag ttcggcccgg
     3241 gccgaatggc ggaacccggc gaaatcctgg ccgccatcga acggcaactg actcccgaca
     3301 cgcggcttgc ggggttgaag gcgctcgtga cggccggccc gacgcaggaa ccgatcgatc
     3361 cggtgcgcta catcgccaac cggtcctcgg ggaagcaggg ctatgccatc gccgcggctc
     3421 tggccgaggc cggcgcgcag accgtactga tttcaggtcc ggtcgagatt gcaaccccgc
     3481 cgggcgtgaa actcgtcaaa gtccagaccg cgcgcgagat gtggtccgcc tgccaatcgg
     3541 agcttcccgc ggacatcgcg gtctgcgcgg cggccgtcgc cgattggcgt ccggaaacca
     3601 ttgcgaacgc caagctgaaa aaacgcaata cggcgccggc tctgccgctg gttgagaatc
     3661 ccgacatcct cgcgggcctt tccagccacg cctcgatgcg tccgaagctg gtgatcgggt
     3721 ttgccgcgga aaccgacgat ctcatcgcga acgcggcggc gaagcgcgtt gccaagggct
     3781 ccgattggat cgtcgccaac gatgtcggcg cgaaggaatc gggggaaagc gtgatgggcg
     3841 gcgattccaa tcgcgtgcac ctgttgacgg caagcggcac ggaagcctgg ccgcaaatgt
     3901 ccaagaccga agtctcgcgg cggctcgccg accgcatcgc ctccgccatc ggacggaacg
     3961 cggcatgaaa ctcgccattc agcggcttgc ccatgggcag gacctgcctc tgccgtccta
     4021 tgcgacggcg ggttccgcgg ggctcgatct ctttgccgcc gtggcgcggg aattcgtgct
     4081 catgcccggc cgctacgcag ccattcccac cggcatcgcg atcgcgcttc ccgacggctt
     4141 cgaagcgcaa ttgcggcccc gctcgggttt ggcgcttcgt catggcatta ccgtattgaa
     4201 cgcgccgggc accatcgatt cggattatcg cggcgaggtg accgcgattc tgatcaatca
     4261 cggcgtcgag ccttttgcga taccgcgcgg aatgaagatc gcgcagttgg tgatcgcggc
     4321 gcatgagcgc atcgaatgga gcgagactac gatgcttgaa agcacacagc gtgggacaga
     4381 cggcttcggc tcgaccggac taggggtcta aggggaatgt ttcgactttc gcgtaagacc
     4441 atgctggcgc tcgaagcggt gatcgacatc gcctataacg cgcggcccga accggtgcag
     4501 gcaaaagaga tcaccgcgcg ccagggtgtg ccgcagcgtt acctcgaaca ggtgatgcag
     4561 cagctcgtgc gcgcgggcat cctgaaaggc gtgcgcggac ctcgcggggg ctaccggctc
     4621 gcccgtgagc gccgccgcat cagcgtcggc gacgtggtgc gcattgccat gagccttgac
     4681 gaaaacgagg agaatttcga cggcggttcg gagcttggcc gccgcgtggt ggtgccgctg
     4741 gtggcatcgc ttcaggaaga ggtgatgaag aaactcgacg aaatcaccat cgaggatatt
     4801 tgtaagcgtg cccgcgtttc aggccttgac ggcggcgtga ccgagcgacc tgataccgcc
     4861 gatttcatca tctgaatggc gtgacgcgaa aggtccccat ggccgacacg attccaaagg
     4921 acaaacgagg cccgcggcac aagccgggcc gaggccgcat ctacaactcc atcaccgaca
     4981 ccatcggcga tacgcctctc gtgcgcatgg tccgcatgcc gcaagccgcc ggcgtgaaag
     5041 cggatcttct gttcaagctc gaattcttca atcccctcgc cagcgtgaag gaccggatcg
     5101 gcgtttcgat gatcgacgcg ctcgaacgcg agggaaaaat cgcgccggga aaaaccgtgc
     5161 tggtggaacc gaccagcggc aatacgggga tcgcgcttgc cttcgtcgcc gcagcgcgcg
     5221 ggtaccatct gatcctggtg atgcccgaat ccatgtcgct ggagcggcgc aagatgctaa
     5281 cgcttctggg agcgcaaatc gagctgaccg agtccgccaa gggcatgaag ggcgcgctcg
     5341 cccgcgccga tcaaatcctc gccacgacgc cgcgttccat catgccgcag caattcgaga
     5401 atcccgccaa ccccgacatt catcgccgca ccaccgcaga agagatctgg aacgacaccg
     5461 agggcaaggt ggatgtggtc atttccgggg tcggcaccgc gggaacgatc accggcgtgg
     5521 gccaggtgct gaaggcgcgc aagccttcgg tcaagatgat cgcggtggag ccagaagaca
     5581 gtccggtact ctcgggcggc cagccaggcc cgcataagat tcaggggctc ggtgcggggt
     5641 tcattccgga aatcctcgac cgctccatca tcgatgaggt ggtcaccatc tcgaatgaga
     5701 ccgctttcga aacggcgcgc gcggccgcgc ggcttgaggg cattcccggc ggcatctctt
     5761 ccggcgcggc aatcgccgcc gctctcgaag tgggcgcgcg cgcgagcatg gcgggaaagc
     5821 agatcgttac gatcattccc tcattcgcgg aacgctacct ctcgacggcc ttgttcgagg
     5881 ggctttagcg cgaaaggatc ttcaacctgt ccgggcgatc cgtgcaaagg gcatcgacgc
     5941 caagccccag cagcgcccgc aactcgcgca catcgttcac cgtccatatt ccgatggaaa
     6001 ggccacacgc gcgcgcggcc tgcacggcct cggtgttggt gcgttcgagc gcgcagaacc
     6061 agccatcgcc gccatgcgcc aggatggcgt ccgcgccgag gcgcgacttt cgctcggtgc
     6121 tgaaccagca cggcaattcc gggtcgagca tcttggcgtg gataagaccc ttccaatcga
     6181 agccaaccag cacggcgaat tcacgaaagg cggccgcgcg caattcgccg accaccgctt
     6241 cggcgacggc ttcggccgat tgcgacaggg tccggtcacc gatgctggtc ttgatctcga
     6301 tgaagaggcg aaaatcctcc tttaccgcgc ggaccgcctc tatcacttcg gacagggacg
     6361 gcatccgttc cccgtcgcgc ggcgtcattt tcgaatgcc
//
LOCUS       DASYEG010000338         3084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2454024, whole genome shotgun sequence.
ACCESSION   DASYEG010000338 DASYEG010000000
VERSION     DASYEG010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2454024"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VGM72_14160"
     CDS             <1..496
                     /locus_tag="VGM72_14160"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266457.1"
                     /translation="AIRIFAMSAAVIFLASACGGPRRGLPARSGDMRAITEQEQNIRL
                     MLSFDGNSDGTVTREEMEAALKRQFDACDTNHDGRIDMREMQVENDRRFRAFGTGASP
                     LIDWNQNGFIEFDEFATTARSVFAELDKNRDGKLDMNELRLPAAGRGGPVVPAENQER
                     RRPM"
     gene            563..1081
                     /locus_tag="VGM72_14165"
     CDS             563..1081
                     /locus_tag="VGM72_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266458.1"
                     /translation="MSRIACLQVAGALVVAVSSVAFAAQRLPAGLPASAALLLRYDAN
                     HDSIVTREEMEAGLKADFAAADSNGDGCLDPAEVRAENQARLARDGAEASPLVDWNLD
                     GCVDLREFSNTAHSYFELADKKKDGKVTQVELRGPSAPLGPRTGAAVRTGMDPAGSTT
                     QTPTNPGTGLPY"
     gene            1190..>3084
                     /locus_tag="VGM72_14170"
     CDS             1190..>3084
                     /locus_tag="VGM72_14170"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4266459.1"
                     /translation="MNDSVLQNATELRRAGKFAEAAQLYAGILKENPRHFEALHALGI
                     LHYQSGRLEDAERLIGEAILINPEAADALYNRASLLLRMNRMDEALHCFDRAIAIRPN
                     YAEALGNRGSALLRIGRTAQGLADFERLVALRPDLPQAWTNHAGALLTLERANDALAS
                     YERALALKPDYADAWRARAGALVALGRQSDALQSVERALQIEPNKAGTLYRRADLLFW
                     LNRHAEAVLAYDAFLAAEPHHADGWNMRGVALVELRRHAEALASFDRAVEAGSNDANA
                     WNNRANVLFELKRFEEAARDYERVLKLAPDLPYVEGFLIQCRIRVCDWRHLDADRQTL
                     SAGLKAGKRIIDPQGNLAISRSPEDQRQCARIFMADEAASPRRWQGGQYAHDRIRVAY
                     LTADFRPHPVAFLVAGVFEQHDRARFETIGFSFGPGTDTDIHARIARAFEHFHDARSN
                     SDAEVAALLKRMEVDIAVDLMAFTEGCRPGILAQRPVPVQVNFLGFPGTSGAAHIDYI
                     LADRILIPESERRFYSEQIVYLPDTYQPNDSRRAIGERTPARVAAGLPEQGFVFCCFN
                     NNYKIMPDMFAIWMRLLRRVEGSVLWLLEDNAAAAANLRAQALEQGIAPERLIFAPRT
                     TPPEHLAR"
BASE COUNT          553 a          936 c          984 g          611 t
ORIGIN      
        1 cgcgattcgg atattcgcaa tgagcgctgc cgtgatcttt cttgcctcgg cgtgcggcgg
       61 gccgcgtcgc ggtcttcctg cgcgttcggg ggatatgcgg gccattaccg agcaggagca
      121 gaatatccgg ctcatgttgt ctttcgacgg aaacagcgac ggcaccgtaa cccgcgagga
      181 gatggaagcc gcgttgaagc ggcagttcga tgcctgtgac accaatcatg acggccggat
      241 cgacatgcgc gagatgcagg tggaaaatga ccgccgcttc cgtgcgttcg ggaccggcgc
      301 ctcgccgctg atcgattgga accagaatgg ctttatcgaa ttcgacgagt tcgcgaccac
      361 ggcacgttcg gtattcgccg agctggataa gaatcgcgac ggcaaactgg acatgaacga
      421 gttgcggctt ccggctgcgg ggcgcggcgg gcccgttgtg ccggcggaaa atcaggaacg
      481 gcgccggccg atgtagccac tgcaccgggt gtctttcccc ggacagcgtg gaggtgtagt
      541 tttaggtgct ggaggactcc tcatgtcgcg tatcgcctgt cttcaagtcg caggggccct
      601 ggtggtcgcc gtctcgagtg tggcgtttgc cgctcagcgg cttcccgcgg gcctccccgc
      661 gagcgcggca ctcctgctgc gatacgatgc caatcacgac agcatcgtca ctcgggagga
      721 aatggaggcg gggttgaagg ccgactttgc cgcggccgac agcaacggag acggttgtct
      781 cgatccggcc gaagtgaggg cagaaaatca ggcgcgtctg gcgcgcgacg gcgcggaggc
      841 ctcacctctg gtggattgga atctggacgg ttgtgtggat ttgcgcgaat tcagcaatac
      901 ggcccattcc tatttcgagc tcgcggataa gaaaaaggac ggaaaggtta cgcaggtgga
      961 actgcgcgga ccctccgccc cgcttgggcc acggaccggc gcggcggtcc gcacggggat
     1021 ggatccagct ggttccacca cgcagacccc gaccaatccg gggaccggtc tgccctatta
     1081 gggccgttcg ctttcacatc cgtgagcttg gcgcgcatcc gccatttccg cccgcggcca
     1141 gaccggctaa gacgagccct gcaagtctgt tgttgatcat gcgggcgcca tgaacgattc
     1201 cgtgctgcag aacgcgaccg agctgcgccg cgctggcaag ttcgccgaag cggcacaact
     1261 ctatgccggc atcctcaagg agaacccccg ccatttcgag gcgctccatg ccttgggcat
     1321 ccttcactac caatcgggca ggctggaaga cgcagaacgg ctgatcggcg aggcgatcct
     1381 catcaatccc gaggccgccg acgcgcttta caatcgggcg tccttgctct tgcggatgaa
     1441 ccggatggac gaagcgctcc attgcttcga ccgggccatc gcgatacggc ccaattatgc
     1501 cgaggcgctg ggcaatcgcg gaagcgctct gctgcgcata gggcgcacgg cccagggtct
     1561 ggccgatttc gaacgccttg tcgcgcttcg cccggatctg ccgcaagctt ggaccaatca
     1621 tgccggtgcg ctgctgacgc ttgagcgtgc gaacgatgcg ttggcgagtt atgaacgtgc
     1681 gctcgcactt aagcccgatt acgccgatgc atggcgcgcg cgggcaggcg cgctggttgc
     1741 gctcgggcgc cagagcgacg cgcttcaatc ggtggagcgc gcacttcaaa tcgagccgaa
     1801 taaagcaggg acgctttatc gccgtgccga tctccttttt tggctcaacc gtcacgcgga
     1861 ggccgtcctt gcctatgacg cgtttctggc ggccgagcct catcatgccg atggctggaa
     1921 tatgcgaggc gttgccctgg tcgagcttcg gcgtcacgcg gaggcgcttg catcattcga
     1981 tcgcgcggtt gaagcaggct ccaatgacgc gaatgcgtgg aacaatcgcg cgaatgtcct
     2041 cttcgagctg aaacgcttcg aggaagccgc gcgcgactat gagcgtgtcc tgaagctcgc
     2101 gcccgatttg ccctatgttg agggcttcct tatccagtgc cgcattcgcg tttgcgattg
     2161 gcgccatctt gatgccgacc ggcagacgtt gagcgcagga ttaaaagctg gaaagcgcat
     2221 catcgatcca cagggcaatc tggcgatttc gcgttctccg gaagatcagc gacaatgcgc
     2281 ccgtattttc atggccgacg aagcggcgtc cccgcgtcga tggcagggcg ggcagtatgc
     2341 gcatgaccgg atccgggtcg cttatctgac ggccgatttc cggcctcatc ccgtggcctt
     2401 tctcgtcgcg ggcgtcttcg agcaacacga tagagcccgt ttcgaaacca ttgggttctc
     2461 gtttggtcct ggaaccgata ccgatattca cgccagaatc gcgcgcgcct tcgaacattt
     2521 ccatgatgcg cgcagcaaca gcgatgcgga ggtggccgca ttgctgaagc gcatggaggt
     2581 cgatatcgcc gtcgatttga tggccttcac cgaaggatgc cgcccgggaa tcctggcgca
     2641 gcgtccggtg cctgtgcagg tgaacttcct gggctttccc ggcaccagcg gtgccgcgca
     2701 tatcgattat attctggcag accgcattct tatcccggaa agcgaacggc gtttctacag
     2761 cgagcagatt gtctatttgc ccgataccta tcagcccaat gattccaggc gcgccatcgg
     2821 cgaacgtacg ccggcgcgcg tcgctgcggg cctcccggaa cagggtttcg tgttctgctg
     2881 cttcaacaac aattataaga tcatgcccga catgttcgcg atctggatgc ggcttcttcg
     2941 ccgcgttgag ggcagcgtgc tttggctgct cgaggacaat gccgcggcgg ccgccaattt
     3001 gcgcgcccag gcgctggagc aggggatcgc gccggagcgc ttgatcttcg cgccgcgcac
     3061 cacgccaccg gagcatctcg cgcg
//
LOCUS       DASYEG010000339         3477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3034717, whole genome shotgun sequence.
ACCESSION   DASYEG010000339 DASYEG010000000
VERSION     DASYEG010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3034717"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..988
                     /gene="trmFO"
                     /locus_tag="VGM72_14175"
                     /pseudo
     CDS             1..988
                     /gene="trmFO"
                     /locus_tag="VGM72_14175"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
     gene            1219..2355
                     /locus_tag="VGM72_14180"
     CDS             1219..2355
                     /locus_tag="VGM72_14180"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4266460.1"
                     /translation="MDRRSFLGAGALAGLAVAAPLTVPASARAAAPDAPTWPSPRGDE
                     EAALEPGLPIVDPHHHLWERRPGVPNFMFPEVLAEIAGSKHKIMQTVFIECGSMYRAD
                     GPRDMRFVGEVEFVNGVAAQSASGNYGPTRIASGIVGRVDFTTGDAVKPVLEALIHAG
                     DGRLRGTRYNAFWTDIPVMGMEHAPLITPKGLLADTKFRQGAAALAGYGLSLDTGCYH
                     PQLMDMVAFVSAIPNVTIVLNHVGGPLTIGRYAAEPAATFTEWKKGMTELAKRPNVNV
                     KLGGLGQNWNYPIGHIDPASNSTALAEKWKPWIETTIELFGVQRCMFESNFPVDKTAA
                     SYDTIWNAFKRITASASADEKAALYAGVARRVYRLPVQTASATP"
     gene            complement(2405..3241)
                     /locus_tag="VGM72_14185"
     CDS             complement(2405..3241)
                     /locus_tag="VGM72_14185"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEY4266461.1"
                     /translation="MPSLDQAACEALVRRVDPDRWLAALFAAAHPRPHLVALYAFYYE
                     VAKTADSVSQPVMGQIRLQWWREAVEEIYAGRARRHEAVQVLAEVVRSHDIPRALLDA
                     MIDAREHDLEETPFTDWVNLEIYADATCGNLMRLAARILGAGPGLDDAAREAGIAYAL
                     MGLLRAFGFHAARRRLMLPVAALHEIGLSQEQIFSGTTDAKVTALFALTVKRTQDHLS
                     RVRASRIPRKFLPALLPAALVPGYAKILTQPGFNPFRDVAELSVHRRQIGMLAAMARA
                     RV"
BASE COUNT          694 a         1122 c         1058 g          603 t
ORIGIN      
        1 attggccgtg gaccgcgacg gattctccgc tgcggtgcag gcggcgctcg aagcccaccc
       61 caatgtcacg ctcgcgcgcg gagaggtcgc cggccctccc gccgattggg acaatgtcat
      121 cgtcgccacc ggcccgctga cctcccaagc gctcgcggag aatttgcgcg cgctcacggg
      181 cgaggattcc ctcgccttct tcgatgccat cgcacccatc gtcgaacgcg attccatcaa
      241 tctcgacatc gcctggttcc aatcgcgcta cgacaagaag ggcccgggcg ggggcgatgc
      301 ggattacatc aattgcccgc tcaacgagac gcaatatcgc ggctttgtcg ccgcactcat
      361 ctccggcgag aagaccgaat tcaaggactg ggaaaaatcc acgccttatt tcgagggctg
      421 cctccccatc gaagtgatgg ccgaacgcgg cgaggacacg ctgagcttcg ggccgatgaa
      481 gccggtgggc ctcagcgatc cgcgtacggg ccggcggccg tttgccgtgg tgcaattgcg
      541 ccaggacaac acgctcggca cgctgtggaa catggtcgga tttcagacca agctgaaaca
      601 tggcgagcag attcgcatct tccgcaccat tccggcgttg gagaacgccc gcttcgcgcg
      661 gctcggcggc atccaccgca atacgttcct gaattcgccg cggctgctcg acggcgaact
      721 gcggctgaaa gcgcagccga gtttgcgttt tgccggtcag atcaccggcg tcgaaggcta
      781 tgtagaatcg gccgccatcg gtttgctggc gggaaggttc gcagcagtgg ccacacgaaa
      841 cgaaacgctc gcgccgcctc cgcccaccac ggcgctgggt gcgcttctca cgcacatcac
      901 caaaggcgcg gacgccgcga cgttccagcc gatgaatgtg aatttcggtc tgttcccgga
      961 gttggagacg cgcacgcgcg ggaaagaccg caagaaagcg atggcccatc gcgcgctcgc
     1021 cgatttcgat gtctggctcg gccgcaaggc agccgcggaa tagactcccg attccccgct
     1081 aagccgcctc ggcgtgcaac gcgattttag tgcttttcca catgcgcact tctttgtgca
     1141 cgtgacaaac ggtgcgcaag ctgaaaaatt agagccagca taaaaaaatt agagccagca
     1201 taaacggagg atttttctat ggatcgccgc agttttctcg gggctggcgc gctcgccggc
     1261 ctagccgtcg ccgcccccct caccgtgcct gcatccgcaa gggctgccgc gccggatgcc
     1321 ccgacttggc caagcccacg cggcgacgag gaagcggcgc tggaaccggg actgcccatt
     1381 gtcgatccgc atcatcacct ttgggaacgg cgcccaggcg tcccaaattt catgttccca
     1441 gaggtcctgg ctgagatcgc cggaagcaaa cacaagatca tgcagaccgt gttcatcgag
     1501 tgcggctcga tgtatcgggc cgacggtccg cgggacatgc ggtttgtggg tgaagtggag
     1561 ttcgtcaacg gcgtcgcggc ccaaagcgcc agcggcaatt acggaccaac cagaattgct
     1621 tcgggcatcg tcggccgcgt ggacttcacc acgggcgatg cggtcaagcc ggtgctggaa
     1681 gcgctgatcc acgcaggcga cggccgcctg agaggcacgc gctacaacgc gttctggacc
     1741 gacatcccgg tgatgggcat ggagcatgcc ccgctcataa ccccaaaggg acttctggcc
     1801 gacaccaagt tccgccaagg cgccgcggcg ctcgccggct atggcttgag cctcgatacg
     1861 ggctgctacc accctcaact tatggacatg gtggcattcg tttcggccat tcctaacgtg
     1921 accattgtgc tgaatcacgt cggcggcccc ctgaccatcg gccgctatgc cgccgagccc
     1981 gccgccacct tcaccgagtg gaaaaagggc atgacagagc tggccaagcg tccgaacgtg
     2041 aacgtgaagc tgggcggtct gggccagaac tggaactatc cgatcggcca tatcgatccg
     2101 gcgagcaact cgactgcgtt ggccgagaag tggaaaccct ggatcgagac gacgattgag
     2161 ctatttggcg tgcagcgttg catgttcgaa agcaatttcc cggtcgataa gaccgccgcc
     2221 agctacgaca ccatctggaa cgccttcaag cgcatcacgg cgagcgcctc ggccgacgaa
     2281 aaggccgcgc tctacgccgg ggtcgcccgc agggtctacc ggctgccggt ccagacggcg
     2341 tcggcaacgc cctgaggctt tgcggcggtc gtcacgttcg accgatcggt gtctcttcga
     2401 atattcacac gcgagcgcgc gccatcgcgg ccagcatgcc aatttggcgg cggtggaccg
     2461 aaagctccgc gacgtcgcgg aacgggttga atcccggctg cgtcaggatt ttcgcatagc
     2521 cggggacaag agccgccgga agcaatgccg gaaggaattt gcgcgggatg cgcgaggcgc
     2581 gcacgcggct taaatgatct tgtgttcgct tgacagtcag ggcgaacagc gccgtcacct
     2641 tggcatccgt cgtgccggag aaaatctgtt cctgcgaaag accgatttca tgcagcgctg
     2701 ccacaggcag catcagccga cgtcgcgcag catggaaacc gaacgcgcgc aacaatccca
     2761 tcagcgcata ggcgatgcct gcctctcgcg ccgcatcgtc gagcccgggt cccgcaccca
     2821 ggatgcgcgc agccagccgc atgaggttgc cgcaggtcgc atccgcatat atctccaggt
     2881 tcacccaatc cgtgaaagga gtttcctcca gatcgtgctc gcgcgcgtcg atcatcgcat
     2941 ccaatagtgc gcgcgggata tcgtgcgaac gaacaacctc ggccaggacc tgcaccgcct
     3001 catgacggcg cgcgcgcccg gcatagattt cctccaccgc ttcgcgccac cattgcaggc
     3061 ggatttgccc catcaccggc tggctgacac tgtccgcggt cttcgcgact tcataataaa
     3121 aggcatagag cgcgacgaga tgcggcctgg gatgcgcggc tgcgaacaat gccgcgagcc
     3181 agcggtccgg gtccactcta cggacgagcg cctcacacgc agcttgatcc agagagggca
     3241 tcggtttcac gcttcgatgt ggaatattcc ctcatcctga gcttggcgaa ggatcgccgc
     3301 aaaaataaaa gggccgcgcg gcagcacagc cctttcggtt gcgggaaacg aacgttcaag
     3361 ccagatgcgg agccgccgcc atgcagaaca acatgggaat cgacagcatc gtattgacgc
     3421 gcgaaaacat gcccgccgag cgtgccgcct tggccttttc ggccgccggc agatcgg
//
LOCUS       DASYEG010000340        17008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4342550, whole genome shotgun sequence.
ACCESSION   DASYEG010000340 DASYEG010000000
VERSION     DASYEG010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 17008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17008
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4342550"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1482)
                     /locus_tag="VGM72_14190"
     CDS             complement(<1..1482)
                     /locus_tag="VGM72_14190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEY4266462.1"
                     /translation="MRFAAIAAGLCLAGCGGGSGGGAPASTIMTPATGTTASTLTPPV
                     SGADAFRTTEYLHMGPLDQIRAADAYALGVTGKGVTIGIVDFNFDLTSSEVNFDPASV
                     GPNPQALALYQAQIGQAASTDMHGLAVAATAAARKNDVGGQGIAFDAKVLAVDYFSDV
                     NETQTVQGGVLIHVSDPWTYITSHGVRIINTSFGYEASDVIPNPPHVSEAYVLASPAV
                     AVVNGALLVSSAGNAGGASPSLSNFDTLSDMQSFGVLNSGPGAFIIVGAVDANNQIAS
                     FSDRAGTAKDHYMVAPGTDFLLPWNGGMAFLSGTSFSAPLVSGAAALIFQRWPNLTAH
                     DVANILFASATDLGAPGVDNIYGNGLLNVAAALQPIGVSTTVVATGIAPAVSATDLVL
                     GPAFGDAPALHRALASVMILDGFGRDFQVDLSSRVGARPNLPDLFGVLEQQLGWRSAN
                     LPIGEATSFSFDMRRNPEDGIVPFQTLAGPQDHLTHVTVMRISG"
     gene            1712..1936
                     /gene="rpmE"
                     /locus_tag="VGM72_14195"
     CDS             1712..1936
                     /gene="rpmE"
                     /locus_tag="VGM72_14195"
                     /inference="COORDINATES: protein motif:HMM:NF001809.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEY4266463.1"
                     /translation="MKKGIHPDYHFVNVQLNDGTSYRTRTTWGESNGKLTLDIDPSTH
                     PAWTGGQQNLLDRGGRLTRFNKRFGDFVKS"
     gene            complement(2039..2557)
                     /locus_tag="VGM72_14200"
     CDS             complement(2039..2557)
                     /locus_tag="VGM72_14200"
                     /inference="COORDINATES: protein motif:HMM:NF018972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HEY4266464.1"
                     /translation="MSLELLEEETSGNLSLNSFRASALFERTFDEGMSLVEECARYLD
                     GRGRTESRALPRKSALVYAGESMRVTTRLMQAASWLLIQRAVQEGDMSAEDAASERYR
                     LGAREICMGKRDEGVEALPGKLIELLDRSESLYRRIARLDDVFFQQEGAPSGVQSQLA
                     RLSAAFGPQNGE"
     gene            complement(2714..2911)
                     /locus_tag="VGM72_14205"
     CDS             complement(2714..2911)
                     /locus_tag="VGM72_14205"
                     /inference="COORDINATES: protein motif:HMM:NF018413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HEY4266465.1"
                     /translation="MAIDLDELMPRKPKVETVLGEDLSAKSAHELEGRIAILEAEIAR
                     TKEALTARAATKNAADAFFWR"
     gene            3002..4000
                     /locus_tag="VGM72_14210"
     CDS             3002..4000
                     /locus_tag="VGM72_14210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEY4266466.1"
                     /translation="MALPKTMNAIAAEPAPGGGTRLFTKEILVPHPGPGQILIKVAAA
                     GVNRADLLQVDGHHPPPPGAPETPGLEASGIVAAVDQTVTGIREGDEVCVLLGGGGYA
                     EYAVASALCSLPVPKGVSLRDAGGLPEVYFTVWSNVFDTARLKQGEDFLVHGGSSGIG
                     TAAIQLMHAKGHRVFTTAGSPEKCAACVKLGATRAIDYRQEDFVALVKAETNGRGVDV
                     ILDMVGGDYVMRNLNTLARSGRLVNIAYQKGAKVEVDFRLVQARLLTLGATGLRGRPD
                     SEKMSIRDSLAREVWPLFEAGRLKPVTHRILPLGEAGAAHQLMRDSSHIGKILLAP"
     gene            4112..4735
                     /locus_tag="VGM72_14215"
     CDS             4112..4735
                     /locus_tag="VGM72_14215"
                     /inference="COORDINATES: protein motif:HMM:NF018001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle transcriptional regulator TrcR"
                     /protein_id="HEY4266467.1"
                     /translation="MSDKPLMPKATAVWLIDNTSLTFEQVAEFCGMHPLEVKGIADED
                     VAKGIKGSDPVAAGQLTHEQIEAAEKNPELRLKMAPAKYKMPYIKQKKTPRYTPVSKR
                     QDKPDAIYWLLRNHPELTDADIMKLIGTTKATIAKIRDRGHWNSQNIKAVDPVTLGLS
                     SQIELDFIVARAAHRKPRVAKRSADGLKTVEETTAGLTPSEDEASED"
     gene            complement(4732..5346)
                     /locus_tag="VGM72_14220"
     CDS             complement(4732..5346)
                     /locus_tag="VGM72_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HEY4266468.1"
                     /translation="MTESTTTGNRGMLRRLYEWTMANASGRHAWMALFAISFAESSFF
                     PIAPDVMLMPMVLADRRRAFLLAAWCTLASVLGGMLGYAIGSLLYDSLGQWVIRVYGY
                     GDRIDEFRSFYAKWGAWVILVKGALPIPYKLITILSGFAGYNFGMFVILSAITRGLRF
                     GAEAALLYRFGEPIRAFIEKRLELAMLLIVGTIIAGIVLAKYVI"
     gene            complement(5343..6593)
                     /locus_tag="VGM72_14225"
     CDS             complement(5343..6593)
                     /locus_tag="VGM72_14225"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4266469.1"
                     /translation="MISPGSSIAAILFATAFFLMGNGLIGTLTPLRAHNQGFSDIAVG
                     ALGAWYYLGFVVGCFAGPHLLARVGHIRAFAVAAVLVAASVLVQPIWTNPAAWFAARG
                     IAGLSMAVLYMAVESWLNDRASNENRGRILSLYIAVNLSALLLGQWLLLLAEPSSFEL
                     FSIGAVLYCLCVVPMGLTRLPAPVLRAPPRIDLIRIITVSPVGAAGCLTVGLANGAFW
                     ALAPIYGQSLGFVTTQIALFMSIFIAGGALIQWPIGRISDSMDRRWMIAATCTIASLC
                     GLVLGVLGWFLIRVPDVFYTIVFVLGASMLPLYSLSIAHANDRLPRAEFVDASAGLLM
                     INAAASVPGPLLASFVIATAGPYSLFLYTALAHAAMAFYAFTRMRTTEPAPPETREIF
                     TPVPPGSPAGLPLDPRGQAQDNTA"
     gene            6740..8494
                     /locus_tag="VGM72_14230"
     CDS             6740..8494
                     /locus_tag="VGM72_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008303469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEY4266470.1"
                     /translation="MATQSKTPIRPKSKKPAKTARVKTAQKAEAPGKPKRKSKALAGN
                     PYETDLERSAANYQPLTPLSFLQRAAGAYPETTAIIHGRQRYSYGEFYARSRRLASAL
                     ARAGVEHGDTVSVLLANTPAMLEAHHGVPMTGAVLNALNTRLDAASVAFMLDHSNAKI
                     FIADLEFAECAKEALGLAKAKPLVIAYDDPEFPQTGTLTGARDYEAFLAGGDPDFAWP
                     MPEDEWDAISLNYTSGTTGDPKGVVCHHRGAALMGYANILACRMAERPVYLWTLPMFH
                     CNGWCFPWTLALIQGTHVCLRWVRAKDMYAAIAEHGVTHLSGAPTVMSMLLNAPPHEK
                     RVFTHPVNFVHAAAPPPEAMLLAMLEAGFSLTHVYGLTETYGPATVNEWHPEWDRLPL
                     KEQGAKRIRQGVAYPALERLAVLDPATLEPVPADGETLGEVMFRGNIVMKGYLKNPKA
                     TRDAMAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENISSIEVENALYRHPAVLF
                     AAVVARPDAKWGETPCAFVEKKPGQENVSEADLIAFCRENLARFKCPKTIIFCELPKT
                     TTGKIQKFRLREQAKTLR"
     gene            complement(8537..8722)
                     /locus_tag="VGM72_14235"
     CDS             complement(8537..8722)
                     /locus_tag="VGM72_14235"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEY4266471.1"
                     /translation="MRLPMALESHVREIADKHQRIQELIETEMTNSAWDEVRVAALKK
                     EKLRLKDELERLRASRH"
     gene            8919..9125
                     /locus_tag="VGM72_14240"
     CDS             8919..9125
                     /locus_tag="VGM72_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEY4266472.1"
                     /translation="MIDEEEQVSRAKLAQLRQEHGDLDAAIAAVAGSARCDQLQLTRL
                     KKRKLQLKDQITQIEDSLLPDISA"
     gene            complement(9151..9876)
                     /locus_tag="VGM72_14245"
     CDS             complement(9151..9876)
                     /locus_tag="VGM72_14245"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY4266473.1"
                     /translation="MKAGKADRTSRIGKIAGSSKPRSAAKAMESGVPPPSATASVLGI
                     PEEEFTVRVRDAIMTLMHEVDRLRKEVDQTRERLEEMARTADQDMLLPILNRRAFVRE
                     ISRFIAFAERYGTPSSLVYFDLDNFKTINDAHGHNAGDFVLHHFADLLQTQIRDSDVL
                     ARIGGDEFAVILAHVTLDQAKKKGASLAQSLREKPAVWNGHVVNLNFSFGAYELQAGA
                     SVDAALEQADRAMYSQKRLGSKI"
     gene            10111..10590
                     /gene="purE"
                     /locus_tag="VGM72_14250"
     CDS             10111..10590
                     /gene="purE"
                     /locus_tag="VGM72_14250"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016659783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEY4266474.1"
                     /translation="MGSRSDWPTMSSAASILGELKVAYETRIVSAHRTPERLYSYATS
                     AKGRGLKAIIAGAGGAAHLPGMTAAMTMLPVLGVPVETRALKGLDSLLSIAQMPGGIP
                     VATFAIGEAGAKNAGLFAAALLALQDAGLAARLDAWRARQTRAVALTPDIKSKRTKR"
     gene            10587..11693
                     /locus_tag="VGM72_14255"
     CDS             10587..11693
                     /locus_tag="VGM72_14255"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004676.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEY4266475.1"
                     /translation="MSAPAVLCAPLAPGAMIGILGGGQLGRMLALAAAKLGFRTHIYS
                     DETESCAFNVATATIRANYDDEEALGRFARACDVVTFEFENVPDATAQYLAEHVPVAP
                     DSRALAVAQDRFLEKSFVAGLGIPTAPFRNVLNAADASDAFAVLGGGPAVLKTRRLGY
                     DGKGQKIVRTSEECVAAFADHRGVPAILEKFVDFTFEASVVAARGRDQIFVAYDPPRN
                     EHEHHILRRSTVPAPLADSTAREAVTIARRIAEALDYVGVLGVELFIGRNGEIAVNEI
                     APRVHNSGHWTMEACAVSQFEQHIRAVAGWPLSDASRHSDAVMKNIIGAEVEAWRALA
                     NEKAGLHIYGKRFVRPGRKMGHITTLSPLGSLRR"
     gene            complement(11696..12328)
                     /locus_tag="VGM72_14260"
     CDS             complement(11696..12328)
                     /locus_tag="VGM72_14260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COQ9 family protein"
                     /protein_id="HEY4266476.1"
                     /translation="MSDAIRDRVLTAALAEIPVTGVSDATLALAAEKCGISKRELGDA
                     FPQGPVSLVEAFSHWADRRMTEILSLQDKQRMRDRITSAVRTRIELMTSHKDATRRVT
                     AFLAQPQHAGLAAKLMMGSVDAMWRVAGDRSSDFSYYTKRATLGGVYGATFAYWLSDS
                     SEGNAATWMFLNHRIDNVMSIEKFKGTARQAMAKLPGPLKIFDALRGRPR"
     gene            complement(12325..12888)
                     /gene="def"
                     /locus_tag="VGM72_14265"
     CDS             complement(12325..12888)
                     /gene="def"
                     /locus_tag="VGM72_14265"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY4266477.1"
                     /translation="MAILKIARMGHPVLAGAAAPVRDPTAPEIKRLVADMIETMEDAN
                     GAGLAAPQVHVPLRLVVFQAPPARAEGGLSEAEAFDHTSPLTVLINPVIEVVDPTTEG
                     GWEGCLSVPGMRGWVERARHIRYTGYDTEGKRILRTAKGFHARVVQHECDHIDGVLYP
                     HRMRDLGKLIFESEIQHWLKRESDDAP"
     gene            complement(12904..13305)
                     /locus_tag="VGM72_14270"
     CDS             complement(12904..13305)
                     /locus_tag="VGM72_14270"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY4266478.1"
                     /translation="MRTKTALTLADAQTMMTAAKAEAQKQKWGVTIAVVDDAGLLLML
                     ERMDGARPQTAEVALLKARSAAITHRPGKTWEETVKTRPGTLNFPDAFPVTGGVPILY
                     HGEVIGAVGVSGVQSSEDEQVAMTGIAALGL"
     gene            13411..13623
                     /gene="rpsU"
                     /locus_tag="VGM72_14275"
     CDS             13411..13623
                     /gene="rpsU"
                     /locus_tag="VGM72_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020041874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEY4266479.1"
                     /translation="MQIIVRDNNIDQALKALKKKMQREGIFREMKLRGHYEKPSEKRA
                     RERAEAIRRYRKLQRKRLQREGLLPR"
     gene            complement(13699..14094)
                     /locus_tag="VGM72_14280"
     CDS             complement(13699..14094)
                     /locus_tag="VGM72_14280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4266480.1"
                     /translation="MNILVVDDEPAILKALQTILTLDGHTVHATVSPHEAIETLRAAD
                     TPFDLTITDQTMPSMSGPELARAVKAVAPSMPIVLLTGVLLTGMPGVSGPDRPPCIDY
                     VLQKPIRLAELRKFMSDFAQRVTAEAGAR"
     gene            14298..16862
                     /locus_tag="VGM72_14285"
     CDS             14298..16862
                     /locus_tag="VGM72_14285"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4266481.1"
                     /translation="MATLRDILRSVSRLIAALTAVVGLLVCLGWAAGIPALKSLFPGF
                     PEMKVNSALAFIFAGLSLWLLNGLRFSRTAARAVALAIVVIALVTLVEYVFGFQAGID
                     QLIAADRTGDSPYPGRIPLTGAVAFALLGTALSSVDVRQKNRWPAQWLAILVSAISFA
                     VILGYAYDVTSIYRARVSGPVALHGAILFFLLGFGVIFARPDRGITARMTAIGASGFL
                     VRRLLPASLIIAPTLGWLRLQGEEAGLYDANVGLALFATANIAIFSGLIWHTAGVVHK
                     ADADRSAAEEKTRGQLARLDLLNHITRAIGERQDPRSIFQVVVRNIEDHLNVDFCCIG
                     LYDRNTVSLTIESVGVRSRGLASELAMPENARISVEGNGLARAVEGVLVYEEDISRLP
                     FPFPKRLSKVGLRALVVAPLITESSVAGVLIACRREPHSFVSSDCEFLRQLSEHVALS
                     THQSQLYAALKQAYDDLRQSQQTVMQQERLRALGQMSSGIAHDINNALTPAALYTEAL
                     MERDDILNADARQQLGIVRRALEDVANTVSRMKEFYGQRETQLRLQPVDLTQCLQHTV
                     DLTRVRWRDFPQSDGIAMELKTDLPASLPNVMGVENEIRDGLTNLIFNAVDAMPQGGT
                     LTVRTFLLAGAPDDSSSSVCVEVIDTGVGMDEDTRRRCMEPFFTTKGERGTGLGLAMV
                     YGMAKRHAAELEVESAPGAGTTIRLVFPAAAQTAHAAAPLATGPVARLRVLLVDDDPI
                     LLQAMYETLTKDGHLVTIAEGGQAGIDTFHAAQDRGVPFDVAITDLGMPYVDGRKVAA
                     ALRAAAPNVHVILLTGWGQRLLAANDTPANVDQVLAKPPKLRELRAALAAASPVRG"
BASE COUNT         3297 a         5245 c         5344 g         3122 t
ORIGIN      
        1 gcctgagatg cgcatgaccg tgacatgggt cagatggtcc tgaggaccgg caagggtctg
       61 gaacggcacg atgccgtctt cgggattgcg ccgcatgtcg aaggaaaatg acgtggcctc
      121 gccgatcggc aaattcgcgg accgccagcc cagttgctgc tccagcacgc cgaagagatc
      181 gggaagattg ggacgtgcgc ccacgcggct cgagagatcg acctgaaaat cgcgcccgaa
      241 gccatcgagg atcatcaccg aggccagcgc gcgatgcaat gcgggcgcat cgccgaaggc
      301 cggtcccaga acgagatcgg tcgcgctcac cgccggcgcg attccggtgg ccacaaccgt
      361 cgttgaaacg ccgatgggtt gcagcgccgc ggcgacattc aaaagtccgt tgccgtagat
      421 gttatcgacg cccggcgcgc ccagatcggt cgccgaagcg aacaggatat tcgccacatc
      481 atgcgccgtc agatttggcc agcgctgaaa gatcagcgcg gcggcgcccg aaaccagcgg
      541 cgccgaaaaa gacgtgccgg agagaaacgc catgccgcca ttccacggca gcagaaaatc
      601 cgtgcccggc gccaccatgt aatgatcttt ggccgttccc gcgcggtcgc tgaacgaggc
      661 gatctggttg ttggcatcga ccgcgccgac gatgatgaaa gcacccggtc cgctgttgag
      721 cacaccgaag gactgcatgt cgctgagggt gtcgaaattg gacaacgacg ggctggcgcc
      781 tcccgcgttg cccgccgagg acaccaacag cgcaccgttg acgacagcga ccgcgggcga
      841 ggcgagtaca taggcttcgc tgacatgcgg cggattgggg atcacatcgc tcgcctcata
      901 accgaacgat gtgttgatga tgcgcacccc gtgcgaggtg atgtaggtcc aggggtcgga
      961 cacatggatg agcacgccgc cctggaccgt ctgcgtttcg ttgacatcgg agaagtaatc
     1021 gaccgccagc accttggcgt cgaacgcgat gccctgtccg ccgacatcgt tcttgcgggc
     1081 cgcggccgtt gccgcgacgg ccagtccgtg catgtccgtg gacgctgcct gtccgatctg
     1141 tgcctggtag agcgccagcg cctggggatt gggtccgacg ctcgcggggt cgaaattcac
     1201 ttcgctggat gtgaggtcga aattgaaatc gacgatgccg atcgtgacac ccttgccggt
     1261 gacgccaagc gcataagcat cggcggcccg gatctgatcg agcgggccca tatgcagata
     1321 ttccgtagtt cggaaagcat cggctccgct caccggcggc gttagcgttg aagccgtcgt
     1381 tccggtcgcg ggtgtcatga tggtgctggc cggcgcgcct ccgcccgaac cgccgccgca
     1441 accggcaagg cacagtccgg ccgcaattgc ggcaaaacgc atgctggttt tcataggccg
     1501 ctttctctgg cgagagctgg aacgggcggc ccgcattgcc cggcaggcat gccgttcgac
     1561 gatgaaagcg atgctaatta gcgccggttc acgcgtccag agcgggccgt ggtaaatatc
     1621 cgtccactat ggcctcggcc gcgcttgcct ccatggcctg catcttctat aagccgccgc
     1681 cttggccgtg agcacacaag gaccgtttgc gatgaaaaaa ggcattcatc cggattacca
     1741 cttcgtgaac gtgcaattga atgacggcac gtcctaccgg acacgcacga catggggcga
     1801 gtccaatggc aagctcaccc tcgacatcga tccgtctacc catccggcat ggaccggcgg
     1861 gcagcagaac ctgctcgacc gtggcgggcg tctcacgcgc ttcaacaagc ggtttggcga
     1921 tttcgtaaag agctgaaact ttccgaatcc ggtcttcgcc ttttcatgcg ctaaccaaaa
     1981 cggcgggcgc ggccgagcaa tcggcacagc gcacggatgt accgattttc ggaaagatct
     2041 actcgccgtt ttgcgggccg aaggccgcgc tcaaacgcgc cagttgcgac tggacgccgg
     2101 acggtgcccc ttcctgttgg aagaagacat cgtcgagccg cgcgatgcgc cggtagaggc
     2161 tttcgctgcg gtccagcaat tcgatcagct tgccgggaag cgcctcgacg ccttcgtcgc
     2221 gctttcccat gcagatttcg cgcgcgccca ggcgatagcg ctcggaggcc gcgtcttcgg
     2281 cggacatatc gccttcctgc acggcgcgct ggatcagaag ccaactcgcc gcctgcatca
     2341 ggcgcgtggt gacgcgcatg ctctccccgg cataaaccag cgcggatttg cgcggcagcg
     2401 cacgcgattc cgttcggccg cgtccatcga gatagcgcgc acattcttcg acgagcgaca
     2461 tgccttcatc gaaggtccgc tcgaaaagcg ccgatgccct gaaactattc agcgacagat
     2521 tgccggacgt ttcctcctcc aagagctcca agctcatagt tcgtccgtct cctgccgtca
     2581 tttcttgtgg gacggaaagc tactccgccg gcaccgcgcg gcatagcgcc cgcgcggaaa
     2641 aattggcgag atggttacga cgcgcggcat cggcggattc tttgccacgc atgttcgtta
     2701 atacagatga atcttaacgc cagaaaaatg catccgccgc attctttgtc gccgcgcgcg
     2761 cggtcagcgc ctctttcgtg cgtgcgattt ccgcttcgag aatcgcgatc cgcccctcca
     2821 gttcatgggc ggattttgcc gagaggtcct cgccgagaac ggtttccact ttcggctttc
     2881 gcggcatgag ttcgtcgaga tcgatggcca tgacgacgtt ctcctttgct ggcttgtttt
     2941 gcactttgcg ttaatcttgg cataagcgaa acactgagca agagagcaag aaagtcgaac
     3001 catggccctg cccaaaacaa tgaatgcgat cgcggccgag ccggcgcccg gcggcggcac
     3061 aaggctattc acgaaagaga ttctcgtccc tcatcctggg cccgggcaga tcctgatcaa
     3121 agtggcggct gcgggcgtca accgggccga tctcctccag gttgacggcc atcatccgcc
     3181 gccgcccggc gcgcccgaaa ccccgggact ggaagcatcg ggcatcgtgg cggccgtcga
     3241 tcagactgtc accggcattc gcgaaggcga tgaggtctgc gtgctgctcg gcggtggcgg
     3301 ctatgccgaa tatgcggtgg catcggcact ttgctcgctg ccggtcccca agggcgtgag
     3361 ccttagagat gccggcggcc tgccggaagt ctatttcacc gtatggagca atgtcttcga
     3421 caccgcgcgg ctgaagcagg gcgaggattt cctggtccat ggcgggtcga gtggaatcgg
     3481 gaccgcggcc atccaattga tgcacgccaa ggggcatcgc gtgttcacca ccgcaggcag
     3541 tccggaaaaa tgcgcggcct gcgtcaagct cggcgcgaca cgcgccatcg actatcggca
     3601 agaggatttc gtcgcgctgg tgaaggccga gaccaatggc agaggtgtcg atgtgatcct
     3661 cgacatggtg ggtggcgatt atgtgatgcg caatctgaat accttggcgc gcagcgggcg
     3721 gctggtgaac atcgcgtatc agaagggcgc caaggtcgag gtcgatttcc ggctggtcca
     3781 ggcccggctc ctgacgctcg gcgcgaccgg gcttcgcggc cggccggatt cggagaagat
     3841 gtcgatccgc gattccctcg cccgtgaggt ctggcccctc ttcgaggccg gccggttgaa
     3901 gccggtgacc caccgcatcc tgccgcttgg cgaggcgggg gcggcccatc agctcatgcg
     3961 cgacagcagc catatcggga aaatcctgct cgccccttga attgtctttt ccgcgagaac
     4021 gttaggcgct aagaagagcc ttcctcgata ggatggtacc ttgagagcgg agtggggtcc
     4081 tgaacagccc cgcacgaaga aggaaaaatt catgtccgac aagcccttga tgccgaaggc
     4141 caccgccgtc tggctgatcg acaacacctc gctcaccttt gaacaggtcg ccgagttctg
     4201 cggcatgcat cccctcgaag tgaagggcat cgccgacgag gacgtggcca aggggatcaa
     4261 gggcagcgat cccgttgccg ccggacaact cacccatgag cagatcgagg cggccgagaa
     4321 aaatcccgaa ctgcggctga agatggcgcc ggcgaaatac aagatgccgt acatcaagca
     4381 gaagaagacg ccgcgctata cgccggtgtc gaagcgccag gacaagcccg acgccatcta
     4441 ttggcttctg cgcaatcatc ccgaattgac cgacgccgac atcatgaagc tgatcgggac
     4501 caccaaggcc acgatcgcga aaatccgcga ccgcggtcac tggaattctc agaacatcaa
     4561 ggccgtagac ccggtgacgc tgggactttc ctcgcagatc gaactcgatt tcatcgtcgc
     4621 gcgggcggcg caccgcaagc cccgcgtggc gaagagatcc gccgacggct tgaagacggt
     4681 cgaagagacg acggcggggc ttacgccatc cgaagacgag gcgagcgagg attagatcac
     4741 gtatttcgcc aggacgatgc ctgcgatgat ggtgccgaca atcagcagca tcgccagttc
     4801 cagccgcttc tcgatgaacg cgcggatcgg ctcgccaaag cggtagagga gtgcggcttc
     4861 cgcgccaaag cgcaaaccgc gcgtgatcgc ggacaggatc acgaacatcc cgaaattgta
     4921 gcctgcgaag ccggacagga tcgtgatcag cttatagggg atcggcagtg cgcctttgac
     4981 gaggatcacc caggcgcccc atttggcgta aaaggacctg aattcgtcga ttctgtcgcc
     5041 atagccgtag acgcggatca cccattggcc gagcgaatcg taaagcagcg agccgatggc
     5101 gtagccgagc atgccgccaa ggaccgaggc gagcgtacac caggccgcca gcagaaacgc
     5161 gcgcctgcgg tcggcaagca ccatcggcat cagcatcaca tcgggcgcga tcggaaagaa
     5221 cgagctttcg gcaaaagaga tcgcgaacag ggccatccag gcatggcgac cggacgcgtt
     5281 cgccatggtc cactcataaa gacgtcgcag catgccgcga ttcccggtgg tcgtgctttc
     5341 ggtcatgcgg tgttgtcctg ggcctggccg cgcgggtcaa gcggcaggcc tgcgggcgag
     5401 cccggcggca ccggcgtgaa gatctctcgg gtctcggggg gagcgggttc ggtcgtgcgc
     5461 atgcgcgtga aggcatagaa ggccatcgcg gcgtgggcga gcgccgtata cagaaatagt
     5521 gaataaggcc cggcggtcgc gatcacgaaa gaggcgagca gggggccggg aacggacgcg
     5581 gccgcattga tcatcaacag gccggccgag gcatcgacga attcggcgcg cggcagccgg
     5641 tcgttggcat gcgcaatcga aagcgaatac agcggcagca tggatgcgcc gagaacaaac
     5701 acgatggtat agaagacgtc cggcacgcgg atcaggaacc agcccaatac gccgagcacc
     5761 agaccgcaca gcgaagcgat cgtgcacgtc gccgcgatca tccagcgccg gtccatgctg
     5821 tccgatatcc ggccgatggg ccattggatc agcgcgccgc ccgcgatgaa gatgctcatg
     5881 aagagggcga tctgggtggt gacgaagccg agcgactgcc catagatggg cgcgagcgcc
     5941 cagaaggcgc cattggcaag gcccaccgtc aggcagcccg ccgcgccaac cggcgaaacg
     6001 gtgatgatgc ggatgaggtc gatacgcggc ggggcgcgca agaccggcgc tggcagccgc
     6061 gtcagtccca tcggcaccac acaaaggcaa tagagcaccg cgccgatgct gaaaagctcg
     6121 aaggatgacg gctcggcgag cagcagcagc cattggccca gaagcagcgc gctgaggttg
     6181 accgcgatat agagtgacag gatccggccg cggttttcat tgctggcgcg gtcgttcagc
     6241 cagctttcca ccgccatata gagaaccgcc atggaaaggc cggcgatccc gcgggctgcg
     6301 aaccaggcgg ccggatttgt ccagatcggc tggaccagaa ccgaggccgc caccagaacc
     6361 gcggccaccg cgaaggcgcg gatgtggccc acgcgcgcca aaagatgcgg acccgcgaag
     6421 cagccgacga caaagcccag ataataccag gcgcccagcg cccccaccgc gatatcggaa
     6481 aatccctggt tatgtgcgcg caacggcgta agcgttccga tgagcccgtt gcccatcagg
     6541 aaaaacgctg tcgcgaacag aatcgcggcg atgctggagc ccggcgaaat catggattcg
     6601 cgtaacctga agagttgcgc tcgctaacct gtttcgcctt tgtacgtcaa aagacgcaat
     6661 tgtggttaca tgcgcctcat gcttgccgtg agaacgccct ttctttagcg ttcacgcggc
     6721 ttttcccgcc ccgggcaaca tggcgacgca atccaagacc cctatcaggc caaaatcgaa
     6781 aaagccggca aaaaccgcgc gcgtcaaaac ggcccaaaaa gccgaagcgc ccggaaaacc
     6841 gaagcgcaag tccaaagcgc tcgcaggcaa tccctatgag accgatctgg agcgcagcgc
     6901 ggccaattat cagccgctga cgcctctgtc gtttctacag cgcgcggcgg gggcctatcc
     6961 cgaaacgacc gccatcatcc atggcaggca gcgatattcc tatggcgaat tctatgcgcg
     7021 ttcgcgccgg ttggcgtcgg cgcttgcgcg tgcaggtgtc gaacacggag acacggtatc
     7081 ggtgcttctt gccaacacgc ccgcgatgct cgaggcccat cacggcgtgc cgatgaccgg
     7141 cgcggtgctg aacgcgctca acacaaggct ggacgcggcg tcggtcgcct tcatgctcga
     7201 tcattcaaac gcgaaaatct tcatcgccga tctggaattc gccgaatgtg cgaaggaggc
     7261 gctcggtctc gcaaaggcaa agccgctcgt catcgcctat gacgatccgg aatttcccca
     7321 aaccgggact ctaaccggcg cgcgcgatta tgaggcgttc cttgcaggtg gcgatccgga
     7381 ttttgcatgg ccgatgcctg aggacgaatg ggatgccatt tccctcaact acacatcggg
     7441 caccaccggc gatcccaagg gcgtggtctg ccatcatcgg ggtgctgctc tgatgggata
     7501 tgcgaatatt ctcgcctgcc gcatggccga gcgtccggtg tatctttgga cgcttccgat
     7561 gttccactgc aatggctggt gttttccctg gacgctcgcg ctgatccagg gaacgcatgt
     7621 ctgtctgcgg tgggttcgtg caaaggacat gtacgcggcc attgcggagc acggcgtcac
     7681 gcatctttcg ggcgcgccga cggtgatgtc gatgctcttg aacgcgcccc ctcacgagaa
     7741 gcgcgtcttc actcatccgg tgaatttcgt gcatgcggcc gcccctcccc ccgaagccat
     7801 gctgctcgcg atgctcgagg ccgggttttc gctcacccat gtctacgggc tgaccgagac
     7861 ctacggtccc gccacggtca atgaatggca ccccgaatgg gacaggctgc cgctgaaaga
     7921 acagggcgcc aaacgcatcc ggcagggggt cgcctacccc gcgctggaac gattggcggt
     7981 gctcgatccg gctacgctgg agccggtgcc ggccgatggc gagacgttgg gcgaggtcat
     8041 gttccgcggc aatatcgtga tgaagggcta tttgaagaat cccaaggcca cccgggacgc
     8101 gatggcggga ggctggttcc attccggcga tctcggagtg atgcaccccg atggctatat
     8161 ccagttgaaa gaccgctcca aggacatcat catttcgggc ggtgagaaca tctcatccat
     8221 cgaagtggag aacgcgctct acaggcaccc tgccgtgctg ttcgccgcgg tggtggcgag
     8281 acccgacgcg aaatggggcg aaacgccgtg cgcgttcgtc gagaagaaac cggggcagga
     8341 aaatgtgtcg gaggcggatt tgatcgcgtt ctgccgcgag aacctcgccc ggttcaaatg
     8401 cccgaagact attatcttct gcgaactgcc caaaacgacc acgggcaaaa tccagaagtt
     8461 ccgtttgcgc gagcaggcaa aaacactccg ctaaggcccc ctatcggagg gcctcgcgag
     8521 ccggaaatgc aaagcctcag tggcgggacg cccgcaatct ttcgagttcg tctttcagcc
     8581 ggagcttttc ttttttcaaa gccgccacgc ggacttcgtc ccacgcggaa ttcgtcatct
     8641 cggtttcgat aagttcttga attctttggt gtttatctgc gatttctctg acgtgtgact
     8701 ctaaagccat cggtagcctc atcgcttgaa ggcatggaat tagagcaccg cgaaaagcgt
     8761 ttgtcacgcc ttgactgaca gacgtgatta gcaaagtccg atcaccgatc cgagagccct
     8821 cctattgcat ccgcgaaagc tatcatcgga gttgcggcag ggttgctcaa aaacggctcg
     8881 acaagcgcaa gtccaaggat catgaagcgt cgcgtgtgat gatcgacgaa gaagaacagg
     8941 tctcgcgggc caagctcgcg cagcttcgcc aggagcatgg cgacctcgat gcggcgatcg
     9001 ctgcggtcgc cggctcggcg cgttgcgacc agctccagct cacgcggctg aagaagcgca
     9061 aattgcagct caaggaccag atcactcaga ttgaggattc gcttctgccc gacatttcgg
     9121 cctgacgcgc aaccggcgaa gagacccgcc ttagattttc gaacccaagc gcttttgtga
     9181 atacatcgcg cgatccgcct gctctaaggc ggcatcgacg ctcgcacccg cctgcagctc
     9241 gtaagcgccg aaggaaaaat tcaggttcac gacgtgcccg ttccacaccg ccggtttctc
     9301 gcgcagggat tgcgcgaggc tggcgccttt tttcttcgcc tggtcgagcg tgacatgggc
     9361 caggatgacc gcgaattcat cgcccccgat gcgggcaagg acgtcgctgt cgcggatctg
     9421 ggtctgcaga agatcggcga aatgatgcaa cacgaaatcg cccgcattgt gaccgtgcgc
     9481 atcgttaatg gtcttgaaat tgtcgaggtc gaaatagacc agactcgacg gtgtgccata
     9541 gcgttcggca aaggcgatga agcgggaaat ctcacgcacg aaggcacgac gattgaggat
     9601 cggcagcagc atgtcctgat cggccgtgcg cgccatttcc tccagacgct cgcgcgtctg
     9661 gtcgacctcc ttccgcaggc ggtcgacctc atgcatcagc gtcatgatcg cgtcgcgcac
     9721 gcgcacggtg aattcctctt ccggaatgcc gagaaccgag gcggtggcgg aaggcggagg
     9781 aacgcccgat tccattgctt tggcggcgct tcggggcttg ctggagccgg cgatctttcc
     9841 gattcggctc gtacgatccg cttttcccgc tttcattccg tcctgctcaa gggcccgtgc
     9901 gctgcgcgat tcgcgcttcg tatgcaaatt gacatcaaat ccatctagcg caaagcgttg
     9961 caataccgct ttcttccaac attttcgccc ctataatcgg ccgcttccga ttcctgcccc
    10021 gcagtttttc tcgcggggaa acccgcgcga agccaaactc tccacatgcc acctcgaaac
    10081 caagcgtcca aacccctcat cggcatcatt atgggaagcc ggtccgattg gccgaccatg
    10141 agcagcgccg cgtctattct gggcgaattg aaagtcgcct atgagacgcg aatcgtctcc
    10201 gcccatcgca cgccggaacg gctctattcc tatgccacaa gcgcgaaagg acgcgggctg
    10261 aaagccatca tcgcgggcgc cggcggagcg gcccatcttc ccggcatgac cgccgccatg
    10321 acaatgctgc cggttctggg tgtcccggtc gaaacgcggg cgctcaaggg gctcgacagc
    10381 ctgctctcca ttgcgcagat gccaggcggc attccggtgg cgaccttcgc catcggtgag
    10441 gcgggcgcca agaacgccgg actttttgcc gcagcgctgc tggcgctcca ggatgccggt
    10501 cttgccgcgc gtctcgatgc ctggcgcgct cggcagaccc gcgcggtggc gcttacgccc
    10561 gacatcaaat cgaagcggac aaaacggtga gcgctcccgc cgtgctttgc gcccctctgg
    10621 cgcccggggc catgatcggc attctcggcg gggggcaatt gggccgcatg ctggcgctcg
    10681 ccgcggccaa gcttggcttt cgcacgcata tttattccga cgagacggag tcctgcgcct
    10741 tcaacgtggc gaccgcgacc atccgggcga attacgacga cgaagaggcc ttgggccgct
    10801 tcgcgcgcgc ctgcgacgtg gtgacgttcg agttcgagaa tgtcccggat gcgacggcgc
    10861 agtacctggc ggagcatgtt ccggtcgcgc ccgattcccg ggccttggcc gtggcgcaag
    10921 accggtttct cgaaaagagc tttgtcgcgg gcctgggaat tccaaccgcg cccttccgca
    10981 acgtgctgaa tgcggcggat gcctccgatg cgttcgctgt gctgggcggc gggcccgccg
    11041 tcctgaagac gcgccggctc ggctatgacg gcaagggcca gaagatcgtg cgcacgtccg
    11101 aggaatgcgt cgccgctttt gccgatcaca ggggcgttcc cgccatcctg gagaaattcg
    11161 tcgacttcac gtttgaggcc tcggtcgtgg cggcgcgcgg acgcgaccag attttcgtgg
    11221 cctatgatcc gcccaggaac gagcacgaac atcacatcct gcgccgttcg acggtgcctg
    11281 cgccgctggc ggattccacg gcgcgcgaag cggtgacaat cgcgcgcaga atcgcggaag
    11341 cgctcgacta tgtcggcgtt ctgggcgtgg agctgttcat cggcaggaac ggcgagatcg
    11401 cggtcaacga gatcgcgccc cgggtgcaca attccggcca ttggacgatg gaagcctgtg
    11461 cggtgagcca gttcgagcag cacatccgcg cggtggcggg ctggcccttg agcgatgcct
    11521 cacggcattc ggacgcggtc atgaagaata tcatcggcgc agaggtggaa gcctggcgcg
    11581 cgctcgcgaa cgagaaggcc ggactgcaca tctatggcaa gagattcgtg cggccgggcc
    11641 gcaagatggg ccacatcacg acgctctccc cactcggaag tttgcggcgt taagctcagc
    11701 gcggccgccc gcgtaacgca tcgaagatct tcagcgggcc ggggagcttt gccatcgcct
    11761 gcctcgctgt gcctttgaat ttttcgatgc tcatcacatt gtcgatgcgg tggttcaaaa
    11821 acatccacgt cgcggcgttc ccctcgctcg aatcgctgag ccaataggca aaggtcgcgc
    11881 catagacacc cccaagcgtg gcgcgcttgg tgtaatagga gaaatcgctc gaacggtcgc
    11941 cggccacgcg ccacatcgca tccacgcttc ccatcatcaa tttggccgcg agccccgcat
    12001 gctgcggctg ggcgagaaat gcggtcaccc ggcgcgtcgc gtccttgtgg gacgtcatca
    12061 gttcgatccg ggttcgcacc gcgctcgtga tgcggtcccg catccgctgc ttgtcctgga
    12121 ggctgaggat ttccgtcatc cgccggtccg cccaatggga gaaggcctcg accagactca
    12181 ccgggccttg cggaaatgcg tcgccaagct cgcgcttcga gatgccgcat ttttccgcgg
    12241 ccagggcgag cgtcgcatcc gacacgccgg tcaccgggat ttcggcaagc gctgcggtca
    12301 gcacgcggtc gcggatcgca tcgctcatgg ggcgtcgtcg ctctcgcgct tcagccaatg
    12361 ctgtatctcg ctttcgaaga tcagcttgcc cagatcgcgc atgcggtggg gatagagcac
    12421 accgtcgatg tggtcgcatt cgtgctgaac gacgcgggcg tggaaccctt tggcggtgcg
    12481 aaggatgcgc ttgccctcgg tgtcatagcc cgtatagcgg atatggcgcg cacgctcgac
    12541 ccagccgcgc atgcccggca ccgaaagaca cccttcccag ccgccttcgg tcgtggggtc
    12601 cacaacttcg atcaccggat tgatgagcac cgtcagcggg gaggtgtggt caaaggcttc
    12661 ggcttccgaa agaccacctt ccgcccgcgc cggaggggcc tggaacacga cgagccgcag
    12721 gggcacgtgc acctgcgggg cggcgagacc ggcgccgttc gcatcctcca tggtctcgat
    12781 catatcggcg accaggcgct tgatctcggg tgcggtcgga tcgcggaccg gcgctgcggc
    12841 gcctgccaga accgggtgcc ccatccgggc gatcttcaga attgccatga gccggacatg
    12901 gggtcagagg ccaagcgcgg caatgccggt catggccacc tgctcatcct cggaactctg
    12961 cacgccggag actccgacag caccaatcac ctcgccgtga taaaggatgg ggaccccgcc
    13021 ggtgaccggg aaggcgtcgg ggaaattcaa cgtgcccggc cgcgtcttga cggtctcttc
    13081 ccaggtcttg ccgggccgat gggtgatggc ggcggaacgg gccttcaaaa gcgccacctc
    13141 ggcggtctgg ggacgggccc cgtccatgcg ttcgagcatc agcagcaggc cggcatcgtc
    13201 cacgaccgcg atggtgaccc cccacttctg cttttgggct tcggccttgg cggcggtcat
    13261 catggtctgg gcgtcggcaa gcgtgagtgc ggtttttgtg cgcataggtt cattcctggc
    13321 agattattgg gatgtggaaa agcccggtag tagacagttt gatggggttc tgataggaac
    13381 ccgcgccgga tttagaagaa agggcctcgc ttgcagatca tcgttcgcga caacaatatc
    13441 gaccaggcgc tcaaggcgct gaagaagaag atgcagcgcg aaggcatctt ccgcgagatg
    13501 aagctccggg gccattacga gaaaccgtca gagaaacgcg ctcgcgagcg cgcggaggcg
    13561 atccgccgct atcgcaagct gcagcgcaag cgcctgcagc gcgaaggcct gttgccgcgc
    13621 tgagtttacg catcctggga gggacaaaag ggccgcccga gggcggtctt ttcgtttgcg
    13681 taagggggca gcgcgaggtc agcgcgcgcc ggcttccgca gtcacgcgtt gtgcgaagtc
    13741 gctcatgaat ttgcgcaact ccgcgagacg gatcggcttt tgcaagacgt aatcgataca
    13801 gggcgggcga tccggaccgc tgacgcccgg cattcctgtc agcaatacgc ccgtcagcaa
    13861 tacgatgggc atcgatggtg ccaccgcctt caccgcgcgc gccaattccg ggccgctcat
    13921 gcttggcatg gtttgatccg tgatcgtcag atcgaagggc gtatctgcag cgcggagcgt
    13981 ttcaatggcc tcatgcggcg agacggtggc atgaaccgta tggccgtcca gggtcaggat
    14041 cgtctgcaac gccttcagaa tcgcaggttc gtcgtcaacg accaggatat tcatggaatc
    14101 gaacctgctt tctgcacgca aagccgcatc ggcccacgct gacttccatc ggtttacaaa
    14161 ccattaacgc gcgcggcgct ctcgcccttc cccggtctta ggctgctacg cgcatcccct
    14221 tgcaatggat cgctaatgcc cgaaggatgc cttgctcgtg tttgagtcgc agggggaaaa
    14281 gcgatgggac gaggcgcttg gctacgctta gggatattct ccggtcggtt tcacggctga
    14341 tcgccgctct tacggcggtg gtcggccttt tggtgtgtct gggttgggcc gccggaatcc
    14401 cggcgctgaa gagcctcttc cccggctttc cggaaatgaa ggtgaattcg gctctcgcat
    14461 tcatattcgc gggactgtcc ctatggcttc tgaacggtct ccggtttagc cggacggccg
    14521 cccgggccgt ggcgctggcg atcgtggtca tcgctctcgt gacgctcgtc gaatatgtgt
    14581 ttggctttca ggcggggatc gatcaactga tcgccgccga ccggaccgga gatagcccct
    14641 atccggggcg catcccgctg accggagcgg tcgctttcgc gctgttgggc acggccttgt
    14701 cgtcggtcga tgtcaggcag aagaatcgtt ggccggccca atggctggcg atcctcgtgt
    14761 cggcgatctc ttttgccgtc atccttggct atgcctacga cgtcacctcg atctatcgtg
    14821 cgcgtgtcag cggaccggtg gctctgcatg gcgcgatcct gtttttcctt ctgggtttcg
    14881 gcgtgatctt tgcccggccc gaccgcggaa tcacagcgcg gatgaccgcc atcggtgcgt
    14941 ccgggttcct ggttcgccgc ttgctgccgg cttcgctgat catagcaccc acgttgggct
    15001 ggctgcgcct gcaaggcgaa gaagccggac tgtacgatgc caatgtgggc cttgcgcttt
    15061 tcgctaccgc gaatatcgcg atcttttccg gattgatctg gcataccgcg ggcgtggttc
    15121 acaaagccga tgcggatcgt tccgcggcgg aagagaaaac gcgcggccaa ctcgcacgtc
    15181 tggacctgtt gaatcatatc acccgcgcca ttggcgaacg gcaggacccg cgcagcatct
    15241 ttcaggttgt cgtgcgcaat atcgaggatc atctgaatgt cgatttctgc tgcatcggcc
    15301 tttacgaccg caacacggtc tcgctgacga tcgaaagcgt gggcgttcgc agccgtggtc
    15361 tggccagcga actcgcgatg ccggaaaacg cacgcatttc cgtggagggc aatggccttg
    15421 cgcgcgccgt ggaaggggtt ctcgtctacg aagaagacat aagccggctg ccctttccgt
    15481 ttccgaaacg gctctcgaaa gtcggtctca gagcgctcgt ggtggcgccc ttgatcaccg
    15541 aaagcagtgt ggccggggtt ttgatcgcct gccggcggga gccgcacagt ttcgtcagct
    15601 ccgattgcga attcctgcgt cagctcagcg agcacgtcgc tctgtccacg catcaatcgc
    15661 aattatacgc cgcgctgaaa caggcctatg acgatcttcg ccaaagccag cagaccgtga
    15721 tgcagcagga acggctgcgc gcgctggggc aaatgtccag tggaatcgcg cacgatatca
    15781 acaatgccct gactccggcg gcgctctaca ccgaagcgct gatggagcgc gacgacatat
    15841 tgaacgccga tgcacgccag caactgggaa tcgtgcggcg cgccctggaa gatgtcgcca
    15901 atacggtatc gcggatgaag gaattctacg gccagcgcga aacccagctc aggcttcagc
    15961 cggtggatct cacccaatgc ctgcagcata cagtcgatct gacgcgtgtt cgctggcgcg
    16021 atttcccgca atccgatggc atcgcgatgg agctgaaaac cgacttgccc gcctctctgc
    16081 cgaatgtgat gggggttgag aacgaaattc gcgacgggct gacaaatctc atcttcaacg
    16141 ccgtcgatgc gatgccccaa ggagggacgc tgacggttcg cacgttcctc ttggccggtg
    16201 cgccggacga ttcctcctca tctgtgtgcg tcgaagtgat cgatacgggg gttggtatgg
    16261 atgaggatac caggcggcgc tgcatggaac ccttcttcac gaccaaaggc gagcggggca
    16321 ccggactggg gctagcgatg gtgtacggga tggcgaaacg ccacgctgca gagctggaag
    16381 tcgaaagcgc gccgggtgcg ggcacgacga tacggctggt cttcccggca gcggcgcaaa
    16441 ccgcccatgc cgctgcgccg ctcgcgacag gcccggtcgc gaggctgcgg gtattgctgg
    16501 tggacgacga cccgattctt ctgcaggcga tgtacgagac cctgacgaaa gacgggcacc
    16561 tggtcacgat tgccgaaggc gggcaggcgg gcatcgacac gttccatgcc gcacaggacc
    16621 ggggcgtgcc gttcgatgtg gccatcaccg atctgggaat gccttatgtc gacggcagaa
    16681 aggtcgccgc cgctttgcgc gccgccgctc ccaatgtgca tgtgatcctg ctcaccggat
    16741 ggggccagcg tttgctcgcc gcaaacgaca ctccggccaa tgtcgatcag gtgcttgcca
    16801 aaccgccgaa gctgcgcgaa ctgcgtgcgg ccttggcggc ggcttcgccg gttcgcggct
    16861 gaaccccagg cgttcaagcc ggggcgcagt cctggcagac cgagacttcg atcgtgacgt
    16921 gggaaagtcc cgaatggcgc cggcgcagtt ccgccttgat gtcgtccgcc gagcagggga
    16981 ccgggctcac cagcgatacg atcagacc
//
LOCUS       DASYEG010000341         1876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4488031, whole genome shotgun sequence.
ACCESSION   DASYEG010000341 DASYEG010000000
VERSION     DASYEG010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4488031"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VGM72_14290"
     CDS             <1..352
                     /locus_tag="VGM72_14290"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HEY4266482.1"
                     /translation="DVVDRGFVTYSNEAKEEMLGVPANILDTHGAVSAPVARAMAEGA
                     LKHSHAQLSVAVTGIAGPGGGSAAKPVGLVYIATASARGETQIRDCRFGDIGRNAVRM
                     ATVKEALTLLRERF"
     gene            complement(354..1244)
                     /locus_tag="VGM72_14295"
     CDS             complement(354..1244)
                     /locus_tag="VGM72_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266483.1"
                     /translation="MQKLLLLTVAALALPAGAASAKTILFIGNSFTMGSGSPVLNYQA
                     DTVHDLNPPNDRGKTLGGVAAIFKEFTKEAGLDYDVSIEAVGGRNFDFHYNERLAVID
                     RPWDVVVMQSYSQLNKDRPGDPAIVTEYGAKLDRAFHAKNPNVQVWFNATWSRADYSS
                     PEIAPWKGKPIDQMAKDVYAGFEKARAASPDVMGIIPVGMAWNRAWATGVADSDPTDG
                     IGPNKLNLWTYDSQHGSSAGYILAGLVIFGQLTGRDPLSLGEREHVAMDLGLSRAQMT
                     ALEHVAHDELAARGVKLAMN"
     gene            complement(1278..1730)
                     /locus_tag="VGM72_14300"
     CDS             complement(1278..1730)
                     /locus_tag="VGM72_14300"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEY4266484.1"
                     /translation="MTEHTESRIVPYTADLMFSVVADVERYPEFLPWVVALRIKSRSK
                     EEDREILMAEMAVGYRALRERYTSRVMLDHQARTIDVVAVEGPFNRLENHWRFTPQAD
                     GTRVDFRVAFEFSNRLLQAAAGQAFEKVLFKMTDAFAARAARLSGPPA"
BASE COUNT          373 a          594 c          554 g          355 t
ORIGIN      
        1 tgatgtggtg gatcgcggct tcgttaccta ttcgaatgag gccaaagaag aaatgctggg
       61 ggttccggcc aatattctgg atacccatgg cgccgtttcg gccccggtgg cacgcgccat
      121 ggcagagggc gcgctgaaac actcccacgc gcaattgagt gtcgcagtca ccggcattgc
      181 cggcccgggc ggcggcagcg ccgcaaagcc ggtcgggctg gtctatatcg cgacagcctc
      241 tgcacgtggc gaaacacaaa tccgcgactg ccggttcggt gatatcggcc gtaacgcggt
      301 tcgtatggca acggtcaaag aagcgctcac gctgctgcgc gagcgcttct gatctaattc
      361 atcgccagct tgacgccgcg cgccgccaat tcgtcgtgcg cgacgtgttc gagtgcggtc
      421 atctgcgcgc gcgaaagccc cagatccatc gccacatgct cgcgctcgcc cagggataac
      481 ggatcgcgcc cggtcaattg cccgaagatc accagaccgg cgagaatgta acccgccgaa
      541 ctgccgtgct gggaatcata ggtccacaga ttgagtttgt tcggaccgat tccgtcggtc
      601 ggatcgctgt ccgccacgcc ggttgcccat gcccggttcc aggccatacc gaccggaata
      661 atccccatca catcaggtga agcggcacgc gctttctcga agcccgcata gacgtccttg
      721 gccatctgat cgatgggctt gcctttccag ggcgcgatct ccggtgagga atagtcggcg
      781 cgcgaccagg tggcgttgaa ccagacctgc acattcggat tcttggcgtg gaacgccctg
      841 tccaacttgg cgccatattc ggtcacgata gcgggatcgc ccggcctgtc cttgttgagc
      901 tgggaatagc tctgcatcac cacaacgtcc cagggccgat cgatcaccgc cagacgctca
      961 ttgtaatgga aatcgaaatt ccgtccgccc acggcctcga tgctcacatc gtaatcgagc
     1021 cccgcttcct tggtgaattc cttgaagatc gccgcgaccc cgcccagagt ttttccgcgg
     1081 tcgttgggcg gattgagatc gtgcaccgtg tcggcctgat agttcagcac cggagagccc
     1141 gagcccatgg tgaagctgtt accgatgaaa agaatggtct tggccgatgc ggcgcccgcc
     1201 ggcagcgcga gtgcggcaac cgtcagcaac aagagttttt gcatgaatgt ctcccccgat
     1261 tttgggagac tatagcctca ggccggcggc cccgaaaggc gggcggcgcg cgccgcaaac
     1321 gcgtccgtca tcttgaacag gaccttctcg aaagcctgcc ccgccgcggc ttgcagcagc
     1381 cggttggaaa actcgaatgc gacgcgaaaa tcgacccgcg tgccgtcagc ttggggcgtg
     1441 aagcgccaat gattttccaa acggttgaaa ggtccttcca cggcaacgac atcgatggtg
     1501 cgcgcctggt gatcaagcat cacccggctg gtatagcgtt cccgcaaggc gcgatagccg
     1561 acagccatct cggccatcag aatctcgcgg tcctcttcct tcgaccgtga cttgatcctg
     1621 agtgcgacca cccagggcag gaattcagga taacgctcca catccgccac gaccgaaaac
     1681 atgaggtcgg ccgtgtacgg aacaatccga ctttccgtat gctcggtcac ttagcctgcg
     1741 cacgcgccgc cttgagcgcg gcgaagtcgc tgtcggcgtg ataggaagag cgcgtcaatg
     1801 gcgacgccga caccatcaaa aagcccttgg cgcgggcgac ctgctccagc ccggcaaatt
     1861 cctgcggcgt ccagaa
//
LOCUS       DASYEG010000342         1935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4488088, whole genome shotgun sequence.
ACCESSION   DASYEG010000342 DASYEG010000000
VERSION     DASYEG010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4488088"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VGM72_14305"
     CDS             <1..352
                     /locus_tag="VGM72_14305"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HEY4266485.1"
                     /translation="DVVDRGFVTYSNEAKEEMLGVPANILDTHGAVSAPVARAMAEGA
                     LKQSHAQLSVAVTGIAGPGGGSAAKPVGLVYIATASARGETQIRECRFGDIGRDAVRM
                     ATVKEALTLLRERF"
     gene            complement(354..1244)
                     /locus_tag="VGM72_14310"
     CDS             complement(354..1244)
                     /locus_tag="VGM72_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266486.1"
                     /translation="MKKFLLLTVAALALPAGAASAKTILFIGNSFTMGSGSPVLNYQA
                     DTVHDLNPPNDRGKTLGGVAAIFKEFTKEAGLDYDVSIEAVGGRNFDFHYNERLTMID
                     RPWDVVVMQSYSQLNKDKPGDPAIVTEYGAKLDKAFHAKNPNVQVWFNATWSRADYSS
                     PEIAPWKGKPIDQMAKDVYAGFEKARAASPDVMGIIPVGMAWNRAWATGVADSDPTDG
                     IGPNKLNLWTYDSQHGSSAGYILAGLVIFGQLTGRDPLSLGEREHVAMDLGLSRAQMT
                     ALEHVAHDELAARGVKLAMN"
     gene            complement(1278..1730)
                     /locus_tag="VGM72_14315"
     CDS             complement(1278..1730)
                     /locus_tag="VGM72_14315"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEY4266487.1"
                     /translation="MTEHTESRVVPYTADLMFSVVADVERYPEFLPWVVALRIKSRSK
                     EEGREILMAEMAVGYRALRERYTSRVMLDPQARTIDVVAVEGPFNRLENHWRFTPQAE
                     GTRVDFRVAFEFSNRFLQAAAGQAFEKVLFKMTDAFAARAARLSGPSA"
BASE COUNT          385 a          601 c          572 g          377 t
ORIGIN      
        1 tgatgtggtg gatcgcggct tcgttaccta ttcgaatgag gccaaagaag aaatgctggg
       61 ggttccggcc aatattctgg atacccatgg cgccgtttcg gccccggtgg cacgcgccat
      121 ggcagagggc gcgctgaaac aatcccatgc gcaattgagt gtcgcagtca ccggcattgc
      181 cggcccgggc ggcggcagcg ccgcaaagcc ggtcgggctg gtctatatcg cgacggcctc
      241 cgcacgtggc gaaacacaaa tccgcgaatg ccggttcggt gatatcggcc gtgacgcggt
      301 tcgtatggcc acggtcaaag aagcgctcac actgctgcgc gagcgtttct gatttaattc
      361 atcgccagct tgacgccgcg cgccgccaat tcgtcatgcg cgacgtgttc gagtgcggtc
      421 atctgcgcgc gcgaaagccc cagatccatc gccacatgct cgcgctcgcc caaggataac
      481 ggatcgcgcc cggtcaattg gccgaagatc accaggccgg cgagaatata acccgccgag
      541 ctgccatgct gggaatcata ggtccacaga ttgagtttgt tcggaccgat gccgtcggtc
      601 ggatcgctgt ccgccacgcc ggttgcccag gcccggttcc aggccatacc gaccggaata
      661 atccccatca catcgggtga agcggcacgc gctttctcga agcccgcata gacgtccttg
      721 gccatctgat cgatgggctt gcctttccag ggcgcgatct ccggtgagga atagtcggcg
      781 cgcgaccagg tggcgttgaa ccagacctgc acgttcggat tcttggcgtg gaacgccttg
      841 tccagcttgg cgccatattc ggtcacgatg gcgggatcgc ccggcttgtc cttgttcaac
      901 tgagagtaac tttgcatcac gacgacgtcc cagggccggt cgatcatcgt cagacgctca
      961 ttgtagtgga aatcgaaatt ccgtccgccc acggcctcga tgctcacatc gtaatccagc
     1021 cctgcttcct tggtgaattc cttgaagatt gcggcaacgc cgcccaaggt ttttccgcgg
     1081 tcgttgggcg gattgagatc gtgcaccgtg tcggcctgat agttcagcac cggagagccc
     1141 gagcccatgg tgaagctgtt gccgatgaag agaatggtct tggccgatgc cgcgcccgcc
     1201 ggcagcgcaa gtgcggccac cgtcagcaac aagaactttt tcatgaatat ctcccccgat
     1261 ttcgggagac tatagcctca ggccgacggc cccgaaaggc gggcggcgcg cgccgcaaac
     1321 gcgtccgtca tcttgaacag gaccttctcg aaagcctgcc ccgcggccgc ttgcaaaaac
     1381 cggttggaga attcgaatgc gacgcgaaaa tcgacccgcg tgccctcagc ttggggcgtg
     1441 aagcgccaat gattttccaa acggttgaaa ggtccttcca cggcaacgac atcgatggtg
     1501 cgcgcctggg gatcgagcat cacccggctg gtatagcgtt cccgcaatgc gcgatagccg
     1561 acagccatct cggccatcag aatctcgcgg ccctcttcct tggaccgtga tttgattctg
     1621 agtgcgacca cccagggcag gaattcggga taacgctcca catccgccac gaccgaaaac
     1681 atgagatcgg cagtgtacgg aacaacccgg ctttccgtat gctcggtcat tctggtcgcg
     1741 ctcgcagacg aaaaaccgct gcacactttt tctgcgagcg ctcggtcact taatctgcgc
     1801 acgcgccgcc ttgagcgcgg cgaagtcgct gtcggcgtga taggaagagc gcgtcaatgg
     1861 cgacgccgac accatcaaaa agcccttggc gcgggcgacc tgctccagcc cggcaaattc
     1921 ctgcggcgtc cagaa
//
LOCUS       DASYEG010000343         9608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_719979, whole genome shotgun sequence.
ACCESSION   DASYEG010000343 DASYEG010000000
VERSION     DASYEG010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 9608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9608
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719979"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="VGM72_14320"
     CDS             <1..1111
                     /locus_tag="VGM72_14320"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEY4266488.1"
                     /translation="RGVTGDTIQHSALLDEIARAISIVVGSRGLALNTPERSQLILDV
                     VNEITGFGPLQPLLDDPTVDDVIVNGPRTIYAERNGELEPVTTRFRNLDHLMNVIQRI
                     VAPIGRRVDESSPYVDGRLPDGSRINVVIPPVALDGPHISIRKFRAVPFTGEDLVHNG
                     SLGPEGLRFLSGAVRARRNILISGGTGSGKTTLLNVLSSYVGNKERIVTIEDTAELQL
                     QMAHVVRMETRAATVDGAREITARDLVRNALRMRPDRIILGEVRSHEAIEVLQAMGTG
                     HDGSMATIHANGTADALERLEMLVGLHGFSSDLRAVRRFIVSAVDIVVQTMRGAGGRR
                     TVSSVVEITGLEGSHYAQREVYRGPEQVERKAVVR"
     gene            1111..2013
                     /locus_tag="VGM72_14325"
     CDS             1111..2013
                     /locus_tag="VGM72_14325"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY4266489.1"
                     /translation="MSVTGTIVAFSCVAFSGLLLARLAAARSRLSDLRRLDTVLAAAP
                     HMAQDTKSESRLPPFVSGILARAGWEPTRRQIVTGAAAMLLLALTAMIASGPLAAISS
                     ALTSVLLGFAFLEYRAAARIRLLAQSMLGFLERIRQLLSVGNSLAVALDRAVENSPPI
                     VGQCLTPTLRRIANGSGVAESLERCAAELDLYELHLLATATRTNLRFGGSMSAILRNI
                     IDNIRKRTSIERELRANTTQIRASAWVLALLPLLVATLVMVTNRDYARWFLSTEKGHG
                     MILYAVASQILGVACMQFVTRTRY"
     gene            2015..2953
                     /locus_tag="VGM72_14330"
     CDS             2015..2953
                     /locus_tag="VGM72_14330"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY4266490.1"
                     /translation="MSLALTWMLCIACGLTGAAVLVFGLRDYAVDQFVARRFREHVSS
                     SLPAPGALARLRVLLERFGQKIAGADSIRQLREMLLQAGYFAPGAAFLFAALRLIATL
                     AAASVMLLRPFLKSGTIAPGDTTLAFFFGFFVYRGFTLFLKMRSESRQREIRRELPYV
                     LDLLLMVLDSGVSIDQALHHVSQQIGRVSPHTAHMLAQYIADTDDGTPYDKALDRLAQ
                     RMAISEGRDFAGLLKQNLFQGGELSQPLRRLVADISEARLANAREQTGRKAVLLTLIM
                     LVFFMPVLMIAIAAPAVTDVLGTLQHVAQDMRGTKQ"
     gene            2950..3663
                     /locus_tag="VGM72_14335"
     CDS             2950..3663
                     /locus_tag="VGM72_14335"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4266491.1"
                     /translation="MKAGYLLFASLLLAGCATTAHKADLAPPPHTDSAMALPAQAELY
                     LGVVDGLIRQKRYEAAIAFLAKYQKTQAPTPRYNKLAGDALTGAGRFDEAITAYRQTL
                     KSSFAPEAYNGIGRALSARGQWAEATENFRQAALLDPSNAGYLNNYAYAQLKQNFQGA
                     DLAPAVSVLQRAYELDPASPLIRTNLALALSLSGSRTQFLAFLGTIPDPAKRKQVADF
                     SVAWKSNWTNDTGIKETMP"
     gene            3660..3917
                     /locus_tag="VGM72_14340"
     CDS             3660..3917
                     /locus_tag="VGM72_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266492.1"
                     /translation="MNRAALFLCVLTIMGLSGCAGTPVHPSPTADQVMAKQYSARGEH
                     GAIGGPEATAITNAYQNAIGKSSQSTPSGETQNEIPAKDSP"
     gene            3936..4124
                     /locus_tag="VGM72_14345"
     CDS             3936..4124
                     /locus_tag="VGM72_14345"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEY4266493.1"
                     /translation="MHDKMKLLFRRFLADESGLSAVEYGLLAAGIAVGLWTLISGMGT
                     SLHSIYTKVQTNLQQAAT"
     gene            4167..4592
                     /locus_tag="VGM72_14350"
     CDS             4167..4592
                     /locus_tag="VGM72_14350"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEY4266494.1"
                     /translation="MPGRGFRQNCRATTAVEFAIIAPFLFAIMFGIIGFGMQFATRIA
                     LTYAATEGGRAAVAGLTDDEREALARGAINKALTALDPLIKLGYATISFDMTAEATDQ
                     KIDIGIAYNDTRFAVLPFVPNFNNQAPVTVSYYVTDPSG"
     gene            4570..6669
                     /locus_tag="VGM72_14355"
     CDS             4570..6669
                     /locus_tag="VGM72_14355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4266495.1"
                     /translation="MSQTRQVEPARRDAAASQRIAPELRRVLDAASIQACHAALADAA
                     QPLCAEFGFSLLTAAEIAPAPQGPAIVWQYDGSLHKSDSHLRAWLARASCNLGRFLAF
                     GDDAAGLVMAGALEADAPMVTLIADCTQLPLPLESAMRDLAEFSARRLYQLRRESETV
                     RRRQRLDMDGARDAALLRASADLIWEAGSDGILHVTHIFQDRRDLARLFEGRVLGDIT
                     VGTRDLQRAAQTGQVLRSLPVTVAGAEEPLFLTACPSSGASAPFPLRGTIAAAPVQVQ
                     ERLAIDTTTLETILASRHREERLRRETETMMLGLRILLGDAPFREKLEQLVKRLASAI
                     DSDEIRLVQFRPGEAPRLVLPEKLAVKEAALAEIATLAGARPITLMPESDQDNARIRD
                     ALNMRNGDIALIALPAPAERYYLLCRARRSLNSDDHGLAERVSLLLQQAILLQADQKQ
                     ILHSAKLSALGQMSTGIAHELRQPLNAISIAAQNIDLMVGLGKAGPEFLQEKTERILA
                     QIDRACKVMDRMRRFGRKTVGDHKPASPAAIARSARSLMDPVIIASGIAVDIEIPDEL
                     EVSADELEVEQVLVNLIQNASDAMAGRPGGKIRIWSRDDPDDPGVIRLEVEDNGPGFP
                     PEVLKHALDAFFTTKPEGKGTGLGLSIAHAILREHGGRLLVGNSEAGGGLITLVLRRP
                     EAAFPSLASRSAMAATP"
     gene            7033..7776
                     /locus_tag="VGM72_14360"
     CDS             7033..7776
                     /locus_tag="VGM72_14360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4266496.1"
                     /translation="MAPVNETILAGDIHILLVDDDSEIRAELGAYLGAHGFKIASAAN
                     SAEAERHMAEKAFDIILLDLWLGSENGFDVLRRLRQRGQTPCIMITAQDEVTDKIVGL
                     ELGADDYMIKPVNLRELLARIRALIRRGGPSGEPAGQPARPPDDHGEPHWKFDPLRRS
                     LNAPDGTSVPLTTAECDLLIELVSKEGMPQTRDELCRRVFNREWSPYDRSLDGIIVKL
                     RRKLEPNPDNPLVIKTVRGRGYVFTGFVR"
     gene            complement(7757..9541)
                     /locus_tag="VGM72_14365"
     CDS             complement(7757..9541)
                     /locus_tag="VGM72_14365"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEY4266497.1"
                     /translation="MLTNARERRRFRAKVSYLARQRAATFARVEMVGKSMKLLAGFTV
                     LLLTGTAALAQSAPPRLSVPDAGHVLTPPLALPAPGPTGPMLPQQPDLPAADQTHLRF
                     TQVEIVGAVHLPEAKIAAAFDAFKGKKISGAELKTALDKVNALYAEAGYPLGRAFVPA
                     QKIKNGILTVRVVEGYIESIAVNADSEKTKVLVERMAARLTAEKPLTRETLEQVILSI
                     QDLPGITLGSKFEAMNPETGGTRLVLAAQVRWVTVGLSLDNRANLQSLPFQPYATATL
                     NNLLGQGDRISLTALLSPRQKDYAFYDLGYSQMIGSDGLNAGLDAAWAQTLDNVSLRP
                     FEVRSRVTRLSTQARYPLKRGTDENINLTGNLYYAGANYSLSHTSVGTFANDRNLALQ
                     LGGDYMRIFSPAFGVTGNASITQGLAGFAEEPHTRLHTIDRFTKLRAEGHAAWQPFEN
                     MTLKFGAMGQYSPNSLIASEEVAFGGLTFGRGFDTSEISGDEGIGFSFQPEYRIALGS
                     GFSITPYPLIDYAKAYNRRNDLQPNGELVSTGVGARFGMDNVATMTLELDKPLNRTPF
                     GRRDRGWRVYAGFEIGVDGALSLIGRNL"
BASE COUNT         1858 a         3032 c         2849 g         1869 t
ORIGIN      
        1 gcgcggcgtg acgggcgaca cgatccagca ttccgcgctt ttggacgaga tcgcccgtgc
       61 catctccatc gttgtcggct cacggggtct ggcgctgaat acgcccgaac gctcccagct
      121 catcctggat gtcgtcaacg agattacggg gtttgggccc ctgcaaccgc tgctcgacga
      181 tcccacggtg gacgatgtga tcgtgaacgg gccgcgaacc atttatgccg aacgcaatgg
      241 cgagctggag ccggtgacca cccgtttccg caatctcgat catctgatga atgttatcca
      301 gcgcatcgtt gcgcccatcg ggcggcgcgt ggacgaatcc agcccgtatg tcgatggccg
      361 cttgccggac ggttcgcgca tcaatgtggt catcccgccg gtagcgctgg atggcccgca
      421 tatctcgatt cgaaaattcc gcgccgttcc cttcaccggc gaagatctgg tgcacaatgg
      481 cagcctgggc cccgaaggtc tgcgctttct gtccggtgcg gtgcgcgcac gccgtaatat
      541 tctgatctcc ggcggtacag gctccggcaa aaccacgctt ctgaatgtct tgagttccta
      601 tgtcgggaac aaggagcgta tcgtcaccat cgaagacacc gccgaacttc aactgcaaat
      661 ggcgcatgtg gtgcgcatgg aaacccgtgc ggcgaccgtg gatggcgcgc gtgaaatcac
      721 cgcgcgcgat ctggtccgca atgccctgcg catgcgcccc gaccggatca ttctgggcga
      781 agtgcgctcg cacgaggcaa ttgaagtgct gcaggccatg ggaaccggcc atgacgggtc
      841 gatggcaacc attcacgcca acggcaccgc cgatgcattg gagcgattgg agatgctggt
      901 cggcctgcac ggtttttcca gcgaccttcg cgcggtacgc cggttcattg tcagcgccgt
      961 cgatattgtg gttcagacca tgcgtggcgc aggcggacgg cgcacagtct ccagcgtggt
     1021 cgaaattacc gggctggaag gatcccatta cgcccagcgt gaagtctatc gcggccccga
     1081 acaagttgag cggaaggctg tggtgcgctg atgtcggtga cgggaaccat cgtcgcgttt
     1141 tcctgtgtcg ctttttccgg tttgctgctg gcgcggctcg ctgccgcgcg gtcgagactg
     1201 tcggacctgc gccggttgga caccgtcttg gcggcagcgc cgcacatggc ccaggacaca
     1261 aaatccgaaa gccgcctgcc gcccttcgtc tccggtattc tggcacgcgc cggttgggag
     1321 ccgacccggc gccagatcgt gacgggcgcc gccgcaatgc tgcttctcgc cctgacggcg
     1381 atgatcgcat ccgggccact tgcggccatc tcgtctgctc tcacgagcgt ccttctggga
     1441 tttgcgtttc tcgaatatcg cgcagccgca cgtattcgcc tgctggcgca atccatgctc
     1501 ggcttcctgg aacgcatccg ccaactgctt tcggtgggca acagcctggc ggtagcgctc
     1561 gatcgggcgg tggaaaattc tccgcccatc gtggggcaat gcctgacgcc cacgctgcgg
     1621 cgcatcgcca atggcagcgg cgtggccgaa agcttggagc gttgcgcggc cgaactcgat
     1681 ctctacgaac tgcatttgct ggcgaccgcg acgcgcacaa atctgcgctt tggcgggtcg
     1741 atgagtgcga tcctgcgcaa catcatcgac aatatccgca aacgcacgtc tatcgagcga
     1801 gaacttcgcg ccaacacgac gcagatccgc gccagtgcct gggtgctggc gctgctgccc
     1861 ctcctggtcg caaccttggt gatggtgacg aaccgcgact atgcgcgctg gtttctgtcc
     1921 accgaaaagg gtcatggcat gatcctctat gccgtcgcgt cgcagatcct gggcgttgcc
     1981 tgcatgcaat tcgtgacacg gacgcggtat tgatatgagc ctggccctga cctggatgct
     2041 ctgtatcgcg tgcggtttga ccggcgcggc tgtgcttgtc ttcggcctgc gggattatgc
     2101 cgtcgatcaa ttcgtggcgc gacgattccg cgagcatgtc tcgtccagcc tgccggcgcc
     2161 cggcgccctc gcgcggctgc gcgtattgct ggagcgattt ggccagaaga tcgcgggcgc
     2221 ggattccatc cgccagctgc gggagatgtt gctgcaagcc gggtatttcg cgcccggcgc
     2281 ggccttcctg ttcgccgcgt tgcgcctgat cgcaaccttg gcagcggcct cggtcatgct
     2341 gctccgcccc tttctcaaaa gcggaacgat tgcccccggt gacaccaccc tcgccttctt
     2401 cttcggtttc ttcgtttatc gcggcttcac gctcttcctg aaaatgcgca gcgaaagccg
     2461 ccagcgcgaa atccggcggg agctgcccta tgttctggat ctcctgctga tggtgctcga
     2521 cagcggcgtg agtatcgacc aggccctgca tcatgtcagc cagcagatcg ggcgcgtcag
     2581 tccccacacc gcccatatgc tcgcgcaata tatcgccgac accgacgacg gcaccccgta
     2641 cgacaaggcg ctcgaccgcc tggcgcaacg catggcgatc agcgagggcc gcgatttcgc
     2701 aggcttgctg aagcagaatt tgtttcaggg tggcgagttg agccagcccc tgcgccgtct
     2761 ggtcgccgac atcagcgagg cccgtctggc caatgcacgc gaacagaccg ggcgcaaagc
     2821 cgtgcttctg accctgatca tgctcgtctt tttcatgccg gttctcatga tcgccatcgc
     2881 cgcgcccgcc gtcaccgacg ttctgggcac attgcaacat gtcgcacagg acatgcgggg
     2941 aacaaaacaa tgaaggccgg atatttgctc tttgcttcgc tgctgctcgc aggatgcgcc
     3001 accactgcgc ataaagccga tttggcgccg ccaccgcaca ccgactcggc catggcgctc
     3061 ccggcccagg ccgagctcta tcttggcgtc gtggacggct tgatccgcca gaagcgctat
     3121 gaagcggcca tcgcgttcct ggcgaaatac caaaagaccc aggcgccgac gccgcgctac
     3181 aacaaactcg cgggcgatgc cttgacgggt gcgggtcgct tcgacgaggc catcaccgcc
     3241 tatcgccaga ccctcaaatc cagcttcgca ccagaggcct ataacggcat cggccgcgct
     3301 ctgtcggcgc gcgggcaatg ggcagaagct accgagaatt ttcgtcaggc cgccctgctg
     3361 gatccctcca atgcaggcta tctcaacaat tatgcttatg cgcaactgaa acagaatttc
     3421 cagggcgccg atttggcacc cgcggtcagc gtattgcagc gcgcctatga actggaccct
     3481 gcctcgccac taatccgcac caatctggcc ctggcgcttt cgctgtccgg ctcccgtacc
     3541 caatttctcg catttctggg cacgatcccc gaccccgcca agcgcaagca ggtcgccgat
     3601 ttttccgtag cctggaaatc gaactggacc aacgacaccg gcatcaaaga gaccatgcca
     3661 tgaaccgggc cgcgctcttc ctgtgcgtcc tgacgatcat gggcctgagc ggttgcgccg
     3721 ggacacccgt gcacccatcg cccacggccg atcaggtgat ggccaaacaa tacagcgcgc
     3781 gcggcgaaca tggcgctatc ggcggccccg aagcgacggc aatcaccaac gcctaccaga
     3841 acgccatcgg caaaagctcg caaagcacgc cgagcggaga aacacagaat gagattccgg
     3901 ccaaggacag tccctaacac aaaggagtgc gtaccatgca tgacaagatg aaattacttt
     3961 tccgccgttt tctcgctgat gagagcggct tgtcggcggt cgaatatggt cttctggcag
     4021 ccggcatcgc tgttggcctc tggaccctaa tatcgggaat gggcacgtcg ctgcactcta
     4081 tctatacgaa ggtgcagacc aatctccaac aggcggcgac ctgaaggcgc agcccgggaa
     4141 tgatgcggcc gctttcacgg acatcaatgc ccggtcgtgg attccggcaa aattgccgcg
     4201 ccacgacagc ggtggaattc gccatcatcg caccgtttct gttcgccatc atgttcggga
     4261 tcataggctt cggaatgcaa ttcgccacgc gcatcgcgct cacctatgcc gcgaccgagg
     4321 gcggacgagc agccgtggca gggctgaccg atgacgaacg cgaagctctg gcgcgcggag
     4381 ccatcaacaa agccctcacc gctctggatc cgctgatcaa gttgggctac gcgaccatca
     4441 gcttcgatat gaccgccgaa gccaccgacc agaagatcga tatcgggatc gcctataacg
     4501 atacccgctt cgccgtgctg ccattcgtac ccaatttcaa caatcaggct cccgtcacgg
     4561 tttcctatta tgtcacagac ccgtcaggtt gagccggcac ggcgtgacgc cgcggcttct
     4621 caacgcatcg caccagaatt gcgccgcgtg ttggacgcgg cctctataca ggcctgccat
     4681 gcggcgctag ctgacgccgc gcagccgctg tgtgcggaat tcgggttttc cctcctgaca
     4741 gcggcggaaa tcgcgcccgc tcctcaaggg ccagccatcg tttggcaata tgacggctct
     4801 ctgcacaaat cggattctca tctgcgcgct tggctcgcgc gcgcaagctg caatctcggg
     4861 cgtttcttgg cttttggcga tgacgccgcc ggcctggtca tggccggcgc tctcgaagcc
     4921 gatgctccca tggtcacctt gatcgcggat tgcacacagc tcccccttcc gctggaaagc
     4981 gcgatgcgcg acctcgcgga attttccgct cgccgcctct atcagttgcg gcgcgagagc
     5041 gagaccgttc gccggcggca aagactggat atggacggcg cccgagacgc ggctctgctg
     5101 cgggctagcg ccgatttgat ttgggaggct ggcagcgacg gcattttgca tgtcacgcat
     5161 atcttccagg atcgccgcga tctggcgcgg ttattcgaag gccgggtact tggcgacatc
     5221 acggtcggca cgcgcgatct gcaacgtgcg gcgcaaaccg gtcaggtatt gcggtcactg
     5281 cccgtcacgg tagcgggcgc cgaggagccg ctctttctga ccgcctgtcc cagctcgggc
     5341 gcatcggcgc cattccccct gcgcggcacc atcgcagcgg cgccggtgca ggtccaggaa
     5401 agactggcca tcgataccac taccctcgaa accattctgg cgtcgcgaca tcgcgaggag
     5461 cggctacgcc gcgagaccga aaccatgatg ctgggcctgc gcatccttct gggcgacgca
     5521 cccttccgcg aaaagcttga gcagttggtc aagcgcctgg cgagcgccat cgacagcgac
     5581 gaaattcgac tggtacaatt ccggccgggc gaagcgccgc gcctggtgct gccggaaaaa
     5641 ctggcagtca aagaggccgc gctggcggag attgccaccc tggcgggcgc gcggccgatc
     5701 acgctgatgc ccgaaagcga tcaggacaat gcgcggatac gcgacgcatt gaatatgcgg
     5761 aatggcgaca tcgcactgat cgcgttgccc gcgccggcgg agcgctatta ccttctctgc
     5821 cgcgcgcggc gcagcctcaa ttccgacgat cacggcctgg cagaacgggt ttcgcttctg
     5881 ttgcagcagg caatccttct acaggccgac caaaagcaaa ttctgcattc cgcgaaattg
     5941 tcggcattgg gccagatgtc gaccggcatc gcacatgaat tgcgccagcc gctcaatgcc
     6001 atttcgatcg cggcccagaa catcgacttg atggtcggcc tgggcaaagc cggacctgag
     6061 tttcttcagg agaagaccga gcgaattctc gcgcaaatcg atcgcgcctg caaagtcatg
     6121 gatcgcatgc gccgctttgg ccgcaagacg gtcggtgatc acaagcccgc gtctcctgcc
     6181 gcaattgccc gcagcgctcg gtctttgatg gatccggtga tcatcgcgtc cggcatcgcg
     6241 gtggatatcg agataccgga tgagttggaa gtctctgcgg acgaattgga ggtcgagcag
     6301 gttttggtga acctgatcca gaacgcatcc gacgcaatgg cgggtcggcc gggcggaaag
     6361 attcgcattt ggtcgcggga tgatcccgac gacccgggcg ttatccgcct tgaagtcgag
     6421 gataatggtc ccggcttccc gccggaggtg ttgaaacacg ccctggatgc tttttttaca
     6481 accaagcccg agggcaaagg cacgggcctg ggcctatcga ttgcccatgc catcctgcgc
     6541 gagcatggcg gacgcctgtt ggtcggcaac agcgaggccg gcggcggatt gatcaccttg
     6601 gtgctgcgcc ggccagaggc cgctttccca tccctcgcct cacggtctgc catggcggcc
     6661 acaccatgac agaggagcgt ccttcgtttt tttgcagtgc ggtcagatcc cagggggcgc
     6721 tgaatcggca cggcaacgcc attcctgaag caaagcaatc agaagaatcc gcaactccgc
     6781 atcatcgagg gaacggattg cacccggtaa agttgtatgg aagacgaatt gcccgatcgg
     6841 ctcctcggca gaaaacgccc cggcaccggg aactttagtt ttatcatgga ccatattgcg
     6901 agccttttca ttgtccccat ccccatacga acacccgcag tatggctcga acggcccccg
     6961 gacaattgtt gagatatgtt tcattgcgta tgggtgcccc tgggcacccc ggaaaccttg
     7021 aggggaaaac tcatggcacc tgtcaacgaa accatcctcg caggcgacat ccatatcctt
     7081 ttggtggatg acgacagcga aattcgtgca gaactcggtg cttatctggg cgcccatggc
     7141 tttaaaattg cctccgcggc caatagcgca gaagctgagc gccacatggc ggaaaaggct
     7201 ttcgacatca ttctgctgga tttgtggttg ggcagcgaga acggcttcga tgtcctgcgc
     7261 cgcctgcgcc aacgggggca gaccccctgc attatgatca cggcacagga tgaggtcacc
     7321 gataagatcg tcgggttgga gctcggcgct gacgactaca tgatcaaacc ggtcaatctg
     7381 cgcgaacttc tggcacgcat tcgggccttg atccggcgcg gcggcccttc cggcgagccc
     7441 gccggtcagc cagcgcggcc gccggacgac catggcgaac cccactggaa gttcgatccc
     7501 ctccgccgca gtctcaatgc gcccgatggc acatcggtgc cgctcaccac ggcggaatgc
     7561 gatctgctga tcgaactggt ttccaaggaa gggatgccgc aaacccggga cgaactctgc
     7621 cgccgcgttt tcaatcgcga atggagtccg tacgaccgta gcctggacgg gatcatcgtc
     7681 aaactccgcc gcaagctcga gcccaatccc gacaatcccc tggtgatcaa aaccgtgcgc
     7741 gggcgcggtt atgtcttcac aggtttcgtc cgataagcga cagcgcaccg tcgacgccaa
     7801 tttcaaagcc tgcataaaca cgccagccac ggtcacggcg gccaaagggc gtgcggttaa
     7861 gcggcttatc cagttccagg gtcatggtgg caacattgtc cattccgaag cgggcgccga
     7921 caccggtcga aacgagctcg ccattgggtt gcagatcgtt gcgccggttg taggccttgg
     7981 cataatcgat caacggatag ggcgtgatac tgaagccgga tcccagcgcg atgcgatatt
     8041 ccggctgaaa tgaaaagccg atgccttcat cgccggaaat ttcggatgta tcgaagccgc
     8101 gtccgaatgt gagcccaccg aacgccacct cttccgaagc aatgagggaa ttggggctat
     8161 attggcccat cgcgccgaac ttcagggtca tattttcaaa aggctgccag gcggcatgcc
     8221 cttcggcacg aagttttgtg aagcggtcga tagtatgcaa acgcgtatgc ggttcttcgg
     8281 caaagcccgc cagcccctgc gtgatcgaag cattgccggt gacgccgaaa gccggcgaga
     8341 aaatccgcat atagtcgccg cccagttgca gcgccagatt gcggtcattg gcgaaggtgc
     8401 cgaccgatgt gtgcgacagg ctgtaattgg cgcccgcata ataaagattg ccggtgagat
     8461 tgatattctc gtccgtgccg cgcttcaaag gatagcgcgc ctgagtcgaa agccgtgtca
     8521 cgcggctacg cacttcgaaa ggccgcaggc tgacattatc gagcgtttga gcccaggccg
     8581 cgtccagacc ggcattgagg ccgtcactgc cgatcatctg ggaataaccc agatcgtaga
     8641 aggcataatc cttctgccgc ggcgacaaca acgccgtcag cgagatgcgg tcgccctgcc
     8701 ccagaagatt gttcaaagtc gcagtggcat agggctggaa gggcagagat tgcagattgg
     8761 cccggttgtc caggctgagg cccaccgtca cccaacggac ttgcgcggcc agaaccaacc
     8821 gtgtgccgcc ggtctccgga ttcattgctt cgaatttcga acccagcgta atgccgggca
     8881 gatcctgtat cgacagaatg acctgctcca gggtttcccg cgtcagaggt ttttctgcgg
     8941 tcaggcgcgc ggccatacgc tcgaccaata ctttggtctt ctcgctgtcc gcattcaccg
     9001 cgatgctttc gatatagcct tcgaccaccc gaacggtgag aatgccattc ttgatttttt
     9061 gcgccggcac gaaggctcgg cccagaggat agcccgcctc cgcgtagagc gcgttgacct
     9121 tgtccaacgc ggtcttcaac tccgcgccag aaatcttttt gcctttgaac gcgtcaaaag
     9181 ccgcggcgat cttcgcttca ggcagatgca cagcgcctac gatctccacc tgggtgaagc
     9241 gcaaatgggt ctgatccgcg gccggcagat cgggctgctg cggcagcatc gggccggttg
     9301 gtcccggcgc cggcaacgcc agcggcggtg tcagaacatg ccccgcatcc ggcacagaca
     9361 gacgcggcgg cgcgctttgc gccaatgccg cagttcccgt caaaagcagt acagtgaatc
     9421 ctgccaggag tttcatcgat ttgcccacca tttccacgcg ggcaaaggtg gcagcgcgct
     9481 ggcgcgcgag atacgagact ttggcgcgaa aacgtcgccg ttcgcgagcg tttgtaagca
     9541 tctgtgagac gcgcaaaaaa gaacccaggc gctttcgcac ctgggttctc ttgccgccga
     9601 atacgccg
//
LOCUS       DASYEG010000344        13726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4343616, whole genome shotgun sequence.
ACCESSION   DASYEG010000344 DASYEG010000000
VERSION     DASYEG010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 13726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13726
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4343616"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VGM72_14370"
     CDS             complement(<1..528)
                     /locus_tag="VGM72_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266498.1"
                     /translation="MMRRIVHAFCAAALAVPFAVGAAAATTQTDRSGAAAQPAGVTLP
                     GVTLIEAAGPRGGGGGGVRGGGGVRGGGGGFRGGAGGFRGGFRGGYRGGFRGGFRGGV
                     FIGPSFGYYDPFYWPYAYPYGYGYYAPPVVVQDGGDYDAPPPDYLPPPDAPPPEQSWY
                     HCSNPDGFYPYVRNCE"
     gene            698..1789
                     /locus_tag="VGM72_14375"
     CDS             698..1789
                     /locus_tag="VGM72_14375"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEY4266499.1"
                     /translation="MTRVLNAAADPRLVKGRGARSNDSSRYDNERRVGFDDGWDHEDE
                     APPPLRTEVMRDATRTIITRNTSPDISFNQSINPYRGCEHGCIYCFARPTHAYLGLSP
                     GLDFESKILVKPDAAGLLAAELRKPSYKPQVIAMGTNTDPYQPLEREHRVTRSILQVL
                     AEFKNPVGIVTKSALIARDIDILAPMAAEGLVKVALSVTTLDRKLARIMEPRAATPER
                     RLETLRALSKAGIPTTVMTAPIIPALNDEEMESILAAAAEAGVSQAGYTLLRLPLEIK
                     DLFREWLEAQVPDRAKHVMSLIRSMRGGKDYDSNWGTRMRGTGPYASLISQRFAIATR
                     RRGLNQIRVPLDTAKFRRPPQTGDQLALL"
     gene            2018..2659
                     /locus_tag="VGM72_14380"
     CDS             2018..2659
                     /locus_tag="VGM72_14380"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEY4266500.1"
                     /translation="MVESLGGPLPHYIFEARWLKTVDGPIAGVDEAGRGPLAGPVVAA
                     AVILDRKRIPKGLNDSKQMTPEAREDAFVQIMACAVVGVGEASVDEIDLVNIRQATHL
                     AMARAVRALPVAPNFALVDGNDAPALPCRYDTLVDGDARSVSIAAASIIAKVTRDRMM
                     RALHAEFPGYGWLTNKGYGTEEHLGALNRLGPCLHHRRSFAPVYSYYLSQRAQ"
     gene            3752..4858
                     /locus_tag="VGM72_14385"
     CDS             3752..4858
                     /locus_tag="VGM72_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEY4266501.1"
                     /translation="MAKKSEGADARVFEADRIIQGDCVTHMNAMPEGVADIVFADPPY
                     NLQLEGELRRPDNSKVDAVDDGWDQFASLGEYDRFTTLWLTAARRVLKPNGALWVIGS
                     YHNIFRVGAILQDLGFWILNDVIWRKSNPMPNFRGMRFTNAHETLIWAAKSREQKSYT
                     FNYEAMKALNDELQMRSDWQLPICSGGERLKDELGGKAHSTQKPESLLHRVILASTSP
                     GDIVLDPFFGSGTTGAVAKRLGRRFVGIERDSNYVRLAQARIDAVEPGDSETIAVTRS
                     KKSEPRIPFGTLVERGLLKPGTVLMDAAGRYRAKVRADGTLVCADATGSIHRIAAHVQ
                     GLDACNGWTFWYYREKGAPFPIDILRQQVRAELN"
     gene            complement(4831..5925)
                     /gene="mutY"
                     /locus_tag="VGM72_14390"
     CDS             complement(4831..5925)
                     /gene="mutY"
                     /locus_tag="VGM72_14390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEY4266502.1"
                     /translation="MTGARASASEALLAWYNANRRDLPWRAKAGEGNDPYRVWLSEIM
                     LQQTTVAAVARYYRAFLERWPDVAQLAAASQDDVLGAWAGLGYYSRARNLHRAAQIVA
                     TQFGGSFPTSAIALRKLPGVGAYTAAAVAAIAFGERAAAMDANAERVIARLFAVETPL
                     PKARPALAALAAPLVPEQRPGDFAQALMDLGSLVCVPKRPFCGECPLAAQCRGRRLGV
                     AEELPRKTAERARPLKRGAAFVALDPLGAVYLVRRPQNGLLGGMLQPPLGEWSEAFPK
                     HSVAVEEAPFMGDWVKKSGFVRHGFTHFQLELEIYVAQFHHRPNGVGAWFSVNDLKHA
                     ALPTLMRKVISHARDDSVPLLAQLSSARTC"
     gene            5961..6449
                     /locus_tag="VGM72_14395"
     CDS             5961..6449
                     /locus_tag="VGM72_14395"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEY4266503.1"
                     /translation="MSRSAKPKSPQGESHPPARHGWVRPAGETVLGEAGHAFARAGFA
                     DATLLLRWTAIAGAEIARIARPVRWQDGPEGATLTLKCEAGAIVFLQHQTRELTERLN
                     AYLGRGRINRLKLVAGKLERKPEVPPHPAPSTGWVAETPDLPGALERLAGARKRLKTS
                     RP"
     gene            6526..7203
                     /locus_tag="VGM72_14400"
     CDS             6526..7203
                     /locus_tag="VGM72_14400"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEY4266504.1"
                     /translation="MNKNTRTYGALAAILVVAAAAGWFAFGRGSSPLAAITGAQSIEI
                     KPTDMVHGSPKAPVTIIEYASMTCPHCAAFQKDVIPLLNKDYVDTGKVKVIFREYPLD
                     GAARMASAVARCLPGDQFFSFIDLLFKNQMNWIKDFDGNGQLTKEDVLEGLTQMGRIA
                     GMPADKVKACSEDPKNLALVDGNWMEGQTKYNVNSTPTFIINGVTHSGEIPYPDLQKI
                     LDPLVKS"
     gene            7237..10692
                     /gene="smc"
                     /locus_tag="VGM72_14405"
     CDS             7237..10692
                     /gene="smc"
                     /locus_tag="VGM72_14405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEY4266505.1"
                     /translation="MKFTRLRLSGFKSFVDSTELFIEPGLTGVVGPNGCGKSNLLEAM
                     RWVMGENRPTSMRGSGMDDVIFGGTNSRPMRNNAEVTLFIDNSDRSAPAPYNEFDVVE
                     VTRRIEREAGSLYRINGREVRQRDVQIFFADASTGSASPALVRQGQISILINQKPLAR
                     RAILEEAAGISGLHQRRHEAELRLKAAETNLGRLNDVIGEVEGQLQGLKRQARQASRY
                     RNLSGLIHKAEALLFLLRWQDLESLAKNAGEALEAVSLLVAQRTQSATHASTRQAEAA
                     ATLPPLRENEAVKGAALQRLLHERNALDAEEARAREDAQRLRQRMGTAEQDRQREGTL
                     EQDAGSALGDLEGELQALENANASAAEKLAEAEEQVQRLSETLAARERDLDRLTAELA
                     QLNATRASYERARNEGGEQAARAQAQRAALQPRADSVLAEIAQTPDVSQAEARVETMR
                     AEVTQARATAEHAETLLGAAAESEGRSRVPLETAERDVQRLDAEIRALTNLLRPQGND
                     LWPPLIDALKVQPGYEAALAAALGDDLDAPLDEAAPQHWRDLDALDGVPPLPAGIALA
                     DFVEAPHALARRLAFTGVVTPEDGPAAQKHLQPGQRLVSQRGDLWRWDGYSASADAPS
                     QAAIRLEQRNRLEALEHERIAAKTVRADAHAIYAMAQNEAAHANEASRAASRALRETE
                     SALIAAQDEAAKAARLAAERAGRLATLETEIKNLDAARETALGAAAAASDALRALPDG
                     EALGAELNNARNLAADSRTATSEARASLETIRRETLARGARLRTVSEEIARWSSRRDA
                     ARRQMETLVTRIAEMQEELAGLEAAPAEIATRRSHLMDAVGTAEQARKETADARAAAE
                     NELAEADRFAKAATLALTEAREERAGCVARAESSAARLTELALRIRDELDTSPDALAA
                     RAELKPDSEIPPLDAAEKRVEKLKAEREQLGGVNLRAEEEALEHEQRLQSLIADREDL
                     DGAIQRLRRGIQSLNREGRERLLESFEKVNANFERLFKELFQGGEARLTFTESEDPLE
                     AGLEILAKPPGKKLTTLSLLSGGEQALTAISLIFAVFLVNPAPICVLDEVDAPLDDAN
                     VDRFCRLLDEMTRLAETRYLIITHHALTMSRMERLFGVTMSERGVSQLVSVSLSEAER
                     VIAAE"
     gene            10776..11099
                     /locus_tag="VGM72_14410"
     CDS             10776..11099
                     /locus_tag="VGM72_14410"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY4266506.1"
                     /translation="MSMDAVFRALADPNRRLLLDRLHARNGQSLNELCEGLGMSRQAV
                     TKHLVILEDANLIAAIRRGRERFHFLNPVPINEIAERWIGKYERGRLQALAQLKKNLE
                     GDHNG"
     gene            11092..11541
                     /locus_tag="VGM72_14415"
     CDS             11092..11541
                     /locus_tag="VGM72_14415"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY4266507.1"
                     /translation="MADDRFIYVTYIRTTQEKVWNALKEPEFTRQYFFGVTVESKWTP
                     DSAWRMVHPDGKVTDSGKVVEIDPPRRLVLSWRNEFKPELTAEGYSRATFELETQGDM
                     VKLTVVHEIAKQGSKLIEAVSGGWPIILSSLKSLLETGKPLERSQSK"
     gene            complement(11543..13558)
                     /locus_tag="VGM72_14420"
     CDS             complement(11543..13558)
                     /locus_tag="VGM72_14420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEY4266508.1"
                     /translation="MARERPPASKDAGPSLRGLHPIRSGGEPPPKAPEGRKSRLRATL
                     LIAGAWVIFAGAILLAHWFSELPNTSNLFAYEPGNDISVMDSKGRLIARRGLTQGESV
                     KVGELPSYVGNAFIAVEDRRFRYHFGIDPVGLARAAFTDMVQGAYVQGGSTLTQQLAK
                     NLFLKPDRTFERKIEEAILAVYLETRYTKDEILTLYLNRVYFGAGVYGIGAASERFFA
                     KPPRELTLTEAAILAGSVKAPSRYNPEASPDAALARSSLVLQAMEDSGFIDAARHKIA
                     AATRPKIAHSFATPGAGYLVDYVVSQVPAFVGKPKERLIVETALDLDLQDLAEKALVR
                     ALAKDGSKLGATQGALVAMTPDGALRALVGGRSYDDSSFDRATEARRQPGSAFKPFVY
                     LAALENGHRPGDIVFDGPVTIGNWKPGNYEGEYEGNVTLAHALAHSSNSAAVQLTNEV
                     GPANVVRIAHRLGIADTLHPVPSLALGTSEVTPLELTGAYAAFANGGIAVAPYAIIRI
                     RTVSGKVLYQRRGSGLSRVMSPESDADMTEMMMGTVTAGTGTAAALKGHDVAGKTGTS
                     QDYRDAWFVGFSADFVTGVWIGNDNGVSMKKATGGGLPARIFKAFMDDAERGQPARPL
                     IGTTLFAAEETPPAEPPMVEDASQHPESPKSDGDVLSAFQDLLDKLF"
BASE COUNT         2755 a         4346 c         4210 g         2415 t
ORIGIN      
        1 ctcgcagttc cgcacatagg gataaaaccc gtccggattc gagcaatgat accagctctg
       61 ctcgggcgga ggggcatccg gtggaggaag ataatcgggc ggcggcgcgt cataatcacc
      121 tccgtcttgc acaacgaccg gcggcgcgta atagccgtaa ccataggggt aggcatacgg
      181 ccaataaaat ggatcgtaat agccgaaaga tggcccgata aacacgccgc ctcggaagcc
      241 accccggaat cctccgcggt aaccacctct gaatccgccc cggaagccgc cggcgccccc
      301 tcgaaatcca ccgccgccac cacggacacc gccgccgcca cggacaccgc cgccgccacc
      361 gccccgtgga ccggccgcct caatcaaagt gacacccggc aaagtgacac ccgccggctg
      421 cgccgcggcg cctgacctat ccgtctgggt ggtcgccgcc gccgcgccaa ccgcaaatgg
      481 cactgccaaa gcggcggcgc aaaacgcatg aacgatccta cgcatcatgt tctcctgaga
      541 cgatcatacc gcggcgtcta cgctgaatgt acggcgcgcc gtgtgcgatt ccagcctgta
      601 ttatttcagc ctcacatgaa cccagactga acgatttaaa gtgcctatcc gacgcaaatg
      661 ttcttgattt gttcttttat ctctttagac tgcatctatg acccgcgtgc tgaatgccgc
      721 cgccgatccg agactcgtca aagggcgcgg cgcccgatcg aacgattcca gccggtacga
      781 caacgaacgc cgggtcgggt tcgacgacgg ctgggatcat gaggacgaag cgcccccgcc
      841 ccttcgaacc gaagtgatgc gcgacgccac ccgcaccatc atcacgcgca atacatcgcc
      901 ggacatctcg ttcaatcaat cgatcaaccc ctaccggggc tgcgagcacg gctgcatcta
      961 ttgtttcgcg cggccgaccc atgcctatct cggcctgtcg cccgggctcg acttcgaaag
     1021 caagatcctg gtcaagccgg atgcggcggg attgctcgca gccgaactgc gcaagccgtc
     1081 ctacaagccg caagtcatcg ccatggggac gaacaccgat ccctatcagc cgctcgaacg
     1141 cgaacaccgc gtgacacgct ccattcttca ggtcctggcc gaattcaaaa acccggtcgg
     1201 catcgtgacg aaatcggcgc tgatcgcgcg cgacatcgac attctggcgc cgatggcggc
     1261 ggaagggctg gtaaaggtcg cactttcggt gacgacgctc gatcgcaagc tcgcgcgcat
     1321 catggagccg cgtgccgcta cacccgagcg ccggctggag accttgcgcg cgctctccaa
     1381 ggcgggcatt cccaccacgg tgatgacggc cccgatcatt cccgcgctca acgatgagga
     1441 aatggaatcc attctggcgg cggcagcgga agccggcgtc agccaggccg gctatacgct
     1501 tttgcgcctg ccgcttgaga tcaaggatct gttccgcgaa tggctggaag cgcaggtgcc
     1561 ggaccgcgcc aagcatgtga tgtcgctgat ccgctcgatg cgcggcggca aggactatga
     1621 ttccaactgg ggcacacgga tgcggggcac cgggccctat gcgagtctga tctcgcagcg
     1681 cttcgccatt gccacgcgcc gtcgtggcct caaccagatt cgtgtgccgc tggacacggc
     1741 caaattccgc cgcccgccgc aaaccggcga tcaactcgcg ctgctttgaa atatccaagc
     1801 cggaaacatc aggcctggaa atatcgggcc cgcattgcaa tggggccgcg acagaggttc
     1861 ggattccttc gcacgctttt tgtcttagat aagaaaagtt acgggaaaac gattcggtct
     1921 gacgggttcg cttaagccgc cgtgccctgg cgatgatgtg ggcaaatggg aaaacgcact
     1981 tgccccacct acccgcttcc gcgctatgaa tttggacgtg gttgaatcgt tgggcggacc
     2041 cttgccgcac tacatcttcg aagcgcgctg gctgaagacg gtggatggcc ctatcgccgg
     2101 cgtggacgaa gccgggcgcg ggccgctggc cgggccggtg gtcgccgccg ccgtcattct
     2161 cgaccgcaag cgcattccca agggcctgaa cgactccaaa cagatgacgc cggaggcccg
     2221 cgaggacgcc tttgttcaga tcatggcatg cgcggtggtc ggcgtcggcg aagcgagcgt
     2281 ggacgagatc gacctcgtca acatccgcca ggcaacccat ctggcgatgg cgcgcgcggt
     2341 gcgggcgctt cccgtcgcgc ccaatttcgc gctcgtcgat ggcaatgacg caccggcctt
     2401 gccctgccgt tacgacacgt tggtcgatgg cgatgcgcgc tcggtgtcca tcgcggcggc
     2461 ctcgatcatc gccaaggtga cgcgcgaccg catgatgcgc gcgctgcatg cggaatttcc
     2521 cggttatggc tggctgacca acaagggcta tggcaccgag gagcatctgg gggcgctgaa
     2581 ccggctgggg ccgtgcctcc atcaccgccg gagttttgca ccggtctatt cttattacct
     2641 atcccaacga gcccaatgaa gttagctcca gcagcgggac gagatagaaa gccgctatcg
     2701 tgagagcagg tcccgtcgaa aaaagtgatc ttccccattt cgaggaccgt ttacgccgct
     2761 cggcgcaaac gaacttttct cagttccaaa gtcccataca cgggctcttg attgcgcaaa
     2821 ctcaggccgg aaaaatttcc ctcagcaaca cctgtgtatt tgtccacttt tagaaacatc
     2881 gtgcctcgcc cttcgctcgt tgggtcttta gggcgatata cgagttcagc atgaccgctt
     2941 ctggagatcg tgcccgagag cacgatggga tttattcgtg ggatgccctt gccgggcgca
     3001 gagcttatca ggagccctcc tccccatacg cgattaccgc gctgtctaaa ttggagtcgc
     3061 gccttgagct tgggcctctc aggttgctcc acaacagccc tatattcgcc tactaagcgt
     3121 ggccccttgc cgattttctg aacctcacgg cccacgacgt catagaatcc cctcaaagct
     3181 tgccgagcag attgcagcct cgacaaaacc gcagggacga ttactgcgaa aataagtgcc
     3241 caagccgcat cgtggtactg cacaaaaagg tcacgcgcct tggaggcgat agccaccacg
     3301 gcgttgttga catcgaaaat gggaccgagc attagaagaa ccggcagaac gactcattct
     3361 ttatatctcg ttaacttggc atttgacagt ctaagcgcgc ttggaccgca tatgctttca
     3421 gctttctgtg ggcaaaaccg ccacggaaat tcgcgagacg gacgtccaaa tcctggatcc
     3481 aattttgatc ccaaccccac ggtacgggac cctatggcca tgactggaag taaagccggt
     3541 gtcgctaatt cgctgatctt cctgacgcct aggggctaaa tgccaccagc cgctagacct
     3601 caatacggag ttttgcaccg gtctaccaga tattgcgtgg tgtgaatcct gaggactcta
     3661 ttttaagcgc ttgattcaat tattcgattg acctgacgaa tcctgtgaat cactgtgccc
     3721 gccctgtgcg taattcgggg gaacatgggg catggctaag aaatctgaag gcgcggacgc
     3781 ccgcgttttc gaagcggacc ggatcatcca aggcgattgc gtgacgcaca tgaacgcgat
     3841 gccggaaggc gtcgccgaca tcgtctttgc cgacccgccc tacaatcttc agcttgaagg
     3901 cgagttgcgg cggcccgaca attccaaggt cgacgcggtg gacgatggct gggaccaatt
     3961 cgcgagtctc ggcgaatatg accgtttcac tacgctttgg ctgaccgccg cgcggcgcgt
     4021 gctcaagccc aatggcgccc tctgggtgat cggctcctat cacaatattt tccgcgtcgg
     4081 cgcgatcctc caagacctcg gtttttggat tctgaacgac gtgatctggc gcaaatccaa
     4141 cccgatgccg aatttccgcg gcatgcgctt caccaatgcg cacgaaaccc tgatctgggc
     4201 cgcgaaatcg cgcgagcaga aaagctacac gttcaattac gaagcgatga aggcgctgaa
     4261 cgacgaactg caaatgcgtt cggattggca attgccgatc tgctccggcg gcgaaagact
     4321 gaaggatgaa ctgggcggca aggcccattc gacccagaag cccgaatcct tgctgcaccg
     4381 ggtgatcctg gcatccacat cgccgggcga catcgtactc gatccgtttt tcggctccgg
     4441 caccacgggt gccgtggcca agagattggg ccgccgtttc gtcgggatcg agcgcgattc
     4501 gaactatgtg cggctggcac aggcgcgcat cgacgccgtc gaacccggcg acagcgagac
     4561 gatcgccgtc acgcgatcga agaaatccga gccgcgtatt ccattcggca cgctggtcga
     4621 gcgcgggctt ctcaaacccg gcacggtcct gatggacgcg gccggacgtt accgggcgaa
     4681 ggtgcgcgcc gacggcacgc tggtgtgcgc cgatgccacc ggctcgatcc atcgcatcgc
     4741 cgcccatgtg cagggactcg atgcctgcaa tggctggacc ttttggtatt accgggaaaa
     4801 gggagcgccc ttcccgatcg atattctgcg tcagcaggtt cgtgccgagc tcaactgagc
     4861 cagaagcggc acgctgtcgt cacgcgcatg tgagatcact ttgcgcatga gtgtcggcaa
     4921 cgcggcgtgc ttcagatcgt ttacggaaaa ccaggcgccc acgccattgg gccggtggtg
     4981 gaattgcgcc acatagattt ccaactccaa ttgaaaatgg gtgaacccat gacgcacgaa
     5041 ccccgatttt ttgacccagt cgcccatgaa gggcgcttct tcgaccgcta cggagtgttt
     5101 cgggaaagcc tcggaccatt cgccgagcgg cggttgcagc atgccgccga ggagaccgtt
     5161 ctgcggccgg cgaacgagat agaccgcgcc cagcggatcg agcgccacga aggccgcgcc
     5221 tcgcttcagg gggcgggcac gctcggccgt ctttcgcggc agctcctcgg caacgcccag
     5281 ccgccgtccc cggcactgcg cggcaagggg gcactcaccg cagaacggac gctttggcac
     5341 gcacacgagg gagccgaggt ccatcagcgc ctgggcaaag tcgcctggac gttgttccgg
     5401 caccaggggc gcggctaacg cggcgagcgc gggcctcgcc ttgggcagcg gtgtttcgac
     5461 ggcaaacagc cgcgcgatga cgcgctcggc attggcgtcc atggcggcgg cccgctcgcc
     5521 gaacgcaatg gccgcgaccg ccgccgccgt ataggcgccg acgccgggaa gtttgcgcaa
     5581 ggcgatggcg ctggtgggaa acgatccccc gaattgagtt gcgacgattt gtgcggcgcg
     5641 atgcagattg cgcgcccggc tgtaatagcc gagtccggcc caggcgccga gcacgtcatc
     5701 ctgggaggcc gcggccaatt gcgccacatc cggccagcgt tcgaggaatg cgcgatagta
     5761 gcgcgcaacc gcggccaccg tcgtctgctg cagcatgatt tcggagagcc agacacggta
     5821 gggatcgttt ccctctccgg ctttcgcgcg ccagggcaga tcgcgcctgt tcgcattgta
     5881 ccaggcgaga agcgcttcgg acgcactcgc gcgggcgccc gtcattcgct tatgctgtct
     5941 ttccttcagg accggcatgg atgagcagaa gcgcgaaacc aaaatccccg caaggagaat
     6001 cgcacccccc cgcccgccac ggctgggtga ggcccgcggg tgagaccgtg ctcggtgagg
     6061 ccggacacgc attcgcgcgc gccggctttg ccgatgcgac gctcctgttg cgttggaccg
     6121 ccatcgcggg cgctgaaatc gcccgtatcg cacggccggt caggtggcag gacggccccg
     6181 aaggcgccac gctcacactc aaatgcgaag cgggcgcgat tgtattcctg cagcatcaaa
     6241 cccgcgaact gaccgaacgg ctcaatgcgt atcttgggcg cgggcgaatc aaccgcttga
     6301 agctggtggc cggcaaattg gaacggaagc ccgaggtgcc gccccatccg gctccctcaa
     6361 ccggctgggt tgcggagacg ccggatttgc ccggcgccct ggagcggctc gccggcgccc
     6421 gcaagcggct caaaacgagc cgcccttaaa ctcaatcatc ccccgattga acctggaccg
     6481 gcgctctgcc acaacagcgg ccgggacttc tggaggaatc cggctatgaa taagaacacc
     6541 cgcacctatg gtgcattggc cgcgatactc gtcgtggccg ccgccgccgg ctggtttgcc
     6601 ttcggccgcg gctcaagccc gctcgccgcg atcaccggcg cgcaaagcat cgaaatcaag
     6661 cccaccgaca tggtccatgg ttcgccgaaa gcgcccgtga ccatcataga atatgcctcc
     6721 atgacgtgcc cgcactgcgc ggccttccag aaggacgtca tcccccttct caacaaggac
     6781 tatgtcgata ccggcaaggt gaaggtgatc ttccgcgaat atcccctcga cggggccgcg
     6841 cgcatggctt cggcggtcgc gcgctgtttg cctggggatc aattcttttc cttcattgat
     6901 ctgctgttca agaatcagat gaactggatc aaggatttcg atggcaacgg tcagctcacc
     6961 aaggaggatg tcctcgaagg cctgacccag atgggccgga ttgcgggcat gcctgccgac
     7021 aaggtcaagg cttgctcgga ggatccaaaa aatctggcgc tggtggacgg caattggatg
     7081 gaaggtcaga ccaaatacaa tgtgaactcg acgccgacat tcatcatcaa cggggtgacg
     7141 cattcgggcg aaattccgta cccagacctg cagaagattc tggatccgct tgtgaagtcg
     7201 taaagcttca agggcgggcg caacaggaac gagacattga agtttacccg tctaaggctc
     7261 agcggcttca aatccttcgt cgattcgacc gagcttttca tcgagcccgg gttgacgggt
     7321 gtcgttggcc ccaatggctg cggtaaatcc aatcttctgg aagccatgcg ctgggtgatg
     7381 ggcgaaaatc gtcccacctc catgcgcggt tccggcatgg acgatgtgat cttcggcggc
     7441 acgaattcgc ggcccatgcg caacaacgcg gaagtcacgc tgttcatcga caattccgac
     7501 cgcagcgcgc ccgcgcccta taatgagttc gacgtcgtgg aggtcacgcg ccgcatcgag
     7561 cgcgaggccg gctccctcta ccgcatcaat ggccgcgagg tccgtcagcg cgacgtgcag
     7621 atcttctttg ccgacgcctc gaccggctcg gcttcacccg ctctggtgcg tcagggccag
     7681 atttccattc tcatcaatca gaagccgctc gcgcgccgcg ccatcctgga agaggcggcc
     7741 ggcatttcag gcctgcatca gcgccgtcac gaggcggaat tgcggctgaa ggcggccgaa
     7801 accaatctcg gacgcctgaa cgatgtcatt ggcgaggtcg aaggccagct tcagggcctg
     7861 aaacgccagg cgcggcaagc atcgcgctac cgcaatctct ccggtcttat ccacaaggcc
     7921 gaggcccttc tgttcctgct gcgctggcag gatttggaaa gcctggcgaa gaacgccggc
     7981 gaagcgctcg aggccgtgtc gctgctggtc gcccaacgca cgcaaagcgc cacccacgcg
     8041 tccacgcgcc aagccgaagc cgcagccact ctgccgccct tgcgcgaaaa cgaggccgtc
     8101 aagggtgcag ccctgcagcg gctgctgcat gagcgcaacg cgctggatgc cgaagaggcg
     8161 cgcgcccgcg aagatgccca gcggctgcgt cagcggatgg gaaccgcgga gcaggatcgt
     8221 cagcgcgagg gcacgctgga acaggatgca ggctccgcgc tcggcgatct cgaaggcgag
     8281 ttgcaggcgc tcgaaaacgc caatgccagc gccgccgaga aactggccga agccgaggaa
     8341 caggttcagc gcctctccga aaccctcgcg gcgcgcgagc gcgatctcga ccggctgacg
     8401 gcggagctgg cgcagctgaa tgcgacgcgc gcgagttatg agcgtgcccg caacgaaggc
     8461 ggcgaacagg ccgcgcgggc gcaagcccag cgcgccgcgc ttcagccgcg cgcggattcc
     8521 gttcttgccg agatcgcgca aacccccgat gtgagtcagg ccgaagcgcg ggtcgaaacc
     8581 atgcgcgcgg aggtaacgca ggcgcgcgcc accgccgaac acgccgaaac acttctgggc
     8641 gcggcagccg aatccgaagg ccgctcccgt gttcctctgg aaaccgcgga gcgcgacgtg
     8701 cagcgcctgg acgcggaaat ccgcgcgctg acgaatctgc tgcgccccca aggcaacgat
     8761 ctttggccgc cgctgatcga cgcgctgaaa gtgcagcctg gctatgaggc cgcattggcc
     8821 gccgcattgg gcgatgatct ggacgcgcct ctggatgaag cggcccctca gcattggcgc
     8881 gatctggatg cgctcgacgg cgtgccgcca ttgcccgccg gcatcgcgct ggccgatttc
     8941 gtcgaagccc cccacgcgct tgcccgccgt cttgcgttca ccggcgtggt cacgccggaa
     9001 gacggccccg ccgcgcagaa acatctgcag ccaggccagc gtctggtctc gcaacgcggc
     9061 gatctgtggc gttgggacgg atattcggct tccgccgatg cgccgagcca ggcggcgata
     9121 cgtctggagc agcgcaaccg ccttgaggcg ctggaacacg aacgaattgc ggcgaaaacg
     9181 gtgcgcgcgg acgcgcacgc gatttatgcc atggcgcaga acgaagcggc gcacgccaat
     9241 gaggcctcac gcgccgcatc gcgcgcgctg cgcgagacag aaagcgcgct catcgccgcc
     9301 caggacgagg ccgccaaggc cgctcgtctc gcggcagagc gcgcgggaag gctcgcgacc
     9361 ctcgaaacgg agatcaagaa tctcgatgcg gcgcgagaga ctgcgcttgg cgccgccgcg
     9421 gcggcgtccg atgcgctccg cgcgctgccc gatggcgaag cgctcggcgc cgaactcaac
     9481 aatgcgcgca atctcgcggc ggactcacgc accgcgacca gcgaggcgcg cgcaagcctc
     9541 gaaaccattc gccgtgagac gctggcgcgc ggagcaaggc tacggactgt gagcgaggag
     9601 atcgcgcgct ggtcttcacg ccgtgacgcg gcccggcgcc agatggaaac tttagtcacg
     9661 cgcatcgccg aaatgcagga agaattggcc ggacttgagg cggcccccgc cgagatcgcg
     9721 acgcggcgtt cgcatctgat ggatgcggtc ggcacggccg aacaagctcg caaggaaacc
     9781 gccgatgcgc gtgcggccgc ggaaaacgaa cttgcggagg ccgaccgatt tgccaaagcc
     9841 gcgacgcttg ccctcaccga agcgcgcgag gaacgcgccg gctgtgtcgc gcgcgccgaa
     9901 tcgagcgccg cccgcttgac ggagcttgcc ctgcgcatcc gcgacgaact cgatacttcg
     9961 cccgatgcgc tcgcggcccg cgccgaattg aaacccgatt ccgagattcc tccactcgac
    10021 gcggcggaga aacgggtgga gaaattgaag gccgaacgtg aacaattagg cggcgtcaat
    10081 ctgcgcgccg aagaagaggc tctggaacat gagcagcgcc tgcaatcgct gatcgccgac
    10141 cgcgaggacc tggatggggc gatccaaaga ttgcgccgcg gcattcaaag cctgaaccgc
    10201 gagggacgcg agcggctgct cgagtctttc gagaaggtga atgcgaattt cgagcgcctg
    10261 ttcaaggaac tgttccaggg cggcgaggca cggcttacct tcaccgaatc cgaagacccg
    10321 ctggaagccg ggctggaaat cctggccaaa ccgccgggca agaagctgac gacattgtcg
    10381 ctgctgtcgg gcggagagca ggcgctgacc gcgatctccc tgatcttcgc ggtattccta
    10441 gtgaaccccg cgccgatttg cgtgctcgac gaagtggatg cgccgcttga cgacgccaat
    10501 gtcgaccgct tctgccggct gttggacgag atgacgcggc tcgccgagac acgctatctc
    10561 atcatcaccc atcacgcgct caccatgtcg cgcatggagc gcctgttcgg tgtcaccatg
    10621 tccgaacgcg gcgtcagtca gcttgtttcg gtgtcgctct ccgaagccga acgcgttatc
    10681 gcggcggaat agttcctgaa attttgaaaa tatttcgcag cgctattcgt gacatggcgg
    10741 cttgacatgc aaccaaatgg ttgcatattg gcgttatgag catggacgct gtgttccgag
    10801 cattggcgga tcccaatcgc cgcctgttgc tggaccggct ccatgcccgc aatggacaaa
    10861 gtttgaacga actgtgcgag ggcctcggta tgagcaggca ggcggtgacc aaacatctgg
    10921 tcattctgga agatgccaat ctgatcgccg cgatcaggcg aggccgcgag cgatttcatt
    10981 ttctcaaccc tgtccccatc aacgaaatcg ccgaacgctg gatcggcaaa tatgagcgcg
    11041 gccgccttca ggcgctcgcc caactcaaga aaaacctcga aggagaccac aatggctgac
    11101 gaccgattta tctatgtcac ctacatccgc accactcagg aaaaagtctg gaacgcgctt
    11161 aaagagccag aattcacgcg ccaatatttt ttcggcgtta cggtggagtc gaaatggacg
    11221 ccggattcgg cgtggcggat ggtccatccc gacggcaagg tgacggacag cggcaaggtg
    11281 gtcgagatcg atccgccgcg ccggctggtc ttgtcatggc gcaatgagtt caagcccgag
    11341 ctgacagcgg aaggttattc ccgcgcgacg ttcgagctcg aaacgcaagg cgacatggtc
    11401 aaactgacgg tcgtccacga gatcgcaaaa cagggctcaa aattgatcga agcggtgtcc
    11461 ggcggctggc cgatcatcct ttcgagcctg aaaagtctgt tggaaaccgg caagccgctg
    11521 gagcggtctc agtcaaaata gctcagaaca atttgtcgag caggtcctga aaggcgctta
    11581 gcacgtcgcc gtcgctcttg ggcgactcgg gatgttgcga agcgtcttcg accatcggtg
    11641 gctctgccgg cggcgtttcc tcggcggcga acaaagtcgt cccgatcaaa ggccgcgcgg
    11701 gctgaccgcg ttcggcatcg tccatgaagg ctttgaaaat gcgcgccggc aagccgccgc
    11761 cggtcgcctt tttcatggag acgccgttgt cgttgccgat ccacacgccg gtcacgaaat
    11821 cggcgctgaa cccgacgaac caggcatcgc ggtaatcctg cgatgtcccg gtcttgcccg
    11881 cgacgtcgtg ccctttgagg gcggcggccg tccccgtgcc cgccgtcacc gtccccatca
    11941 tcatctcggt catgtcggcg tcgctttccg gactcatcac gcgcgagagg ccggagccgc
    12001 ggcgctgata gagcaccttg ccggatacag tacgtatacg gataatcgcg tagggcgcga
    12061 cggcgatgcc gccattggcg aaggcggcat aggcgccggt caattcgagc ggtgtgactt
    12121 cggaggtgcc cagtgcgagc gagggcacgg gatgcagcgt gtccgcgatt cccagccgat
    12181 gcgcgatccg cacgacattc gccggaccca cttcattggt gagctgaacc gccgcggaat
    12241 tggaagaatg agcgagcgca tgagcaagcg tgacattgcc ctcatattcg ccttcgtaat
    12301 ttccgggttt ccaattgccg atcgtcaccg gcccgtcgaa aacgatatcg ccggggcgat
    12361 ggccgttttc cagggcggcg agataaacga agggcttgaa cgccgagccc ggttgacgcc
    12421 gggcttccgt ggcgcggtcg aatgagctgt cgtcatagga acgtccaccg accagcgcac
    12481 gcagcgcgcc gtccggcgtc atcgcaacca gcgcgccctg cgtcgcgcca agcttggagc
    12541 catccttcgc gagtgcgcgt accagcgcct tctcggccag gtcctgcagg tcgagatcca
    12601 gcgcggtttc gacgatcagc cgttccttcg gcttgcccac gaaggcggga acctgcgaaa
    12661 cgacatagtc caccaaatat cccgcaccgg gcgtcgcgaa gctgtgcgcg atcttgggtc
    12721 gcgtcgcggc tgcaattttg tggcgcgccg catcgatgaa ccctgaatcc tccatcgcct
    12781 ggagcaccaa ggaggatcgg gcgagcgcgg cgtcgggcga tgcttcggga ttgtagcgcg
    12841 agggcgcttt cacactgccc gcaagaatcg cggcctcggt aagggtcaat tcacgcgggg
    12901 gcttggcgaa gaagcgttcc gacgccgcgc cgatgccata gaccccggca ccgaaataga
    12961 cgcggttcag atagagcgtc aggatctcat ccttggtgta ccgggtttcc agataaaccg
    13021 cgaggatcgc ttcctcgatc ttgcgctcga aggtgcggtc cggtttgagg aacagattct
    13081 tggcaagctg ctgggtgagc gtggagcctc cctggacata ggcgccctgg accatatcgg
    13141 taaaggccgc gcgcgccagt cccaccggat cgatgccgaa atgatagcgg aagcggcggt
    13201 cttccaccgc gatgaaggcg tttccgacat aggacggaag ctcaccgacc ttgacgcttt
    13261 ctccctgcgt caggccccgg cgcgcgatca gacggccctt cgaatccatt acggagatgt
    13321 cgttccccgg ctcataggca aacaggtttg acgtattggg aagctctgaa aaccaatgcg
    13381 cgagcaggat ggcgccggcg aaaatcaccc acgccccggc gatgagcaga gttgcgcgaa
    13441 gccgcgattt tcttccttcg ggcgcttttg ggggcggctc gccgccggag cggatgggat
    13501 gcaggccgcg caaagaaggg cccgcatcct tggacgccgg aggacgttcg cgagccattg
    13561 tatcgccttg attcccctct aatccccaga gccaaggctc attaaacatc cagaatggcg
    13621 aaaacgaggc ggcagcgggc gacattgtgc caagacgtcc ctacataggg atagacttga
    13681 acctcatcca ctgcgcctga acccgttatg aacgaacaaa cgcaaa
//
LOCUS       DASYEG010000345         9606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_720284, whole genome shotgun sequence.
ACCESSION   DASYEG010000345 DASYEG010000000
VERSION     DASYEG010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 9606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9606
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720284"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="VGM72_14425"
     CDS             <1..1111
                     /locus_tag="VGM72_14425"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEY4266509.1"
                     /translation="RGVTGDTIQHSALLDEIARAISIVVGSRGLALNTPERSQLILDV
                     VNEITGFGPLQPLLDDPTVDDVIVNGPRTIYAERNGELEPVTTRFRNLDHLMNVIQRI
                     VAPIGRRVDESSPYVDGRLPDGSRINVVIPPVALDGPHISIRKFRAVPFTGEDLVHNG
                     SLGPEGLRFLSGAVRARRNILISGGTGSGKTTLLNVLSSYVGNKERIVTIEDTAELQL
                     QMAHVVRMETRAATVDGAREITARDLVRNALRMRPDRIILGEVRSHEAIEVLQAMGTG
                     HDGSMATIHANGTADALERLEMLVGLHGFSSDLRAVRRFIVSAVDIVVQTMRGAGGRR
                     TVSSVVEITGLEGSHYAQREVYRGPEQVERKAVVR"
     gene            1111..2013
                     /locus_tag="VGM72_14430"
     CDS             1111..2013
                     /locus_tag="VGM72_14430"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY4266510.1"
                     /translation="MSITGTIVALSCVAFSGLLLVRLAAARSRLSDLRRLDTVLAAAP
                     HMAQDAKSESRLPPFVSGILARAGWEPTRRQILAGATATIALTLAAMAVSGPLAAGST
                     AITLIFISFAFLEYRAVARIKLLSQSMLGFLERIRQLLSVGNSLAVALERAVENSPPI
                     VGQCLAPTLRRIANGSGVAESLERCATELDLYELHLLATATRTNLRFGGSMTAILRNI
                     IENIRRRTSIERELRANTTQIRASAWVLALLPILVATLVMWTNRDYARWFLSTEKGHS
                     MILYAVISQIMGIGCMRLVIRTRY"
     gene            2015..2953
                     /locus_tag="VGM72_14435"
     CDS             2015..2953
                     /locus_tag="VGM72_14435"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY4266511.1"
                     /translation="MAPLLTWTLCIACGLTGGIALVHSLRDYALDQSVARRFREHVSP
                     SLPAPGMRAQLRTLLERFGRRIAGTDSIRQLRDALLQAGYFAPGAAFLFAALRLIATL
                     TAASVLLARPFLKYGTIASGDAAISFFFGFFIYRGFTIFLKLRSESRQRDVRRELPYV
                     LDLLLMVLDSGVSIDQALQHVSQQIGRVAPLTAHMLSQYIADTDDGMPYDKALDRLAQ
                     RMAISEGRDFAGLLKQNLFQGGELGQPLRRLAADISEARLSNAREQTGRKAVLLTLIM
                     LAFFMPVLMIAIAAPAVTDVLGTLQHIASDMQGKKK"
     gene            2950..3660
                     /locus_tag="VGM72_14440"
     CDS             2950..3660
                     /locus_tag="VGM72_14440"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4266512.1"
                     /translation="MKAGFLIFTLFLTGCAAAPHASGPARPQPADVAMTPPAQAELYL
                     GVIDGLIRQKRYEAAIAFLAKYQKTEPLTPRYNKLAGDALTGARRFDEAIAAYCHTLK
                     SNFAPEAYNGIGRALSARGQWPEAAENFRQAAILDPSNAGYLNNFAYAQMKQNFQGAD
                     LAPVVSELQRAHELDPASPLIRTNLALALSLSGSRDQFLAFLNTIPDAAKRKQVADFS
                     TAWKADWTGDTGIKESTP"
     gene            3657..3914
                     /locus_tag="VGM72_14445"
     CDS             3657..3914
                     /locus_tag="VGM72_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266513.1"
                     /translation="MTRIAFLLCGVALTGLSGCASSAVRPSPTTDQVMAEQYRAQGEH
                     GSISGPETAAIADAYRKAIGKSTQAAPNSEMRNDVPIENSP"
     gene            3933..4121
                     /locus_tag="VGM72_14450"
     CDS             3933..4121
                     /locus_tag="VGM72_14450"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEY4266514.1"
                     /translation="MRDTIKNLTCRFLADESGLSAVEYGLLAAGIAVGLWTLISGMGT
                     SLRAIYTTVQGNLSEATP"
     gene            4158..4589
                     /locus_tag="VGM72_14455"
     CDS             4158..4589
                     /locus_tag="VGM72_14455"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEY4266515.1"
                     /translation="MEDFCHRFRRSRCATTAIEFAIVAPFLFAIMFGIIGFGMQFATH
                     IALTYAATEGGRAAVAGLNDGERETLARGAITKALTALKPLIKVGNATISFDMTAEAT
                     DQKINIGIAYNDTRFAVLPFVPNFNNQAPVTVTYYVTDPSG"
     gene            4645..6666
                     /locus_tag="VGM72_14460"
     CDS             4645..6666
                     /locus_tag="VGM72_14460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4266516.1"
                     /translation="MLSATSVQACHKGLADAAQLLDAEFGFSTLAVAEIASTPQGHAV
                     VWQTDGPLRGSGPLLRAWLERASRNPGRSLAFGDAATGLLMAGAPEGDAPMVALIADG
                     TLLPPPLESAMRDLAEFSARHLCHLRRESEILRRRQRLDIDGARDTALLRANADLIWE
                     ADSDGILHVTHIFQDRRDLAKLFEGRVLSDIGVGASDLQSAAKSGQVLRSLPVTVTGA
                     SGPIFLTACPTLGPSAPFPLRGTIATALEQARERLVIDTHTLETILASRHREEQLRRE
                     TETMMMGLRILLGDSPFREKLEQLVRRLADAIDGDEVRLVLFRPGEAPRLVLPEGRGE
                     ENSALTDIASLAGQRTVTLLRQTGHDNARICKALAMRNGDIVLVALPAAAERYYLLCR
                     TRRNLNSDDHGLAERVSLLLQQAILLQADQKQILHSAKLSALGQMSTGIAHELRQPLN
                     AISIAAQNIDLMVGLGKAGPEFLQEKTERILAQIDRACKVMDRMRRFGRKTVGEYKPA
                     SAAAIARSARSLMDPVIISSGIAVDIEVPDDLEVLADELEIEQVLVNLIQNASDAMTG
                     RAGGRIRIWARDDFADPSVVKLEVEDNGSGFPPEILKHALDAFFTTKPEGKGTGLGLS
                     IAHAILREHGGRLLVSNSEAGGGLITLILRRPEARILSFAPRPVTAGVP"
     gene            complement(6700..7032)
                     /locus_tag="VGM72_14465"
     CDS             complement(6700..7032)
                     /locus_tag="VGM72_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266517.1"
                     /translation="MGVSLKGSWGAWERPYAMKHISTIVWTLAGPYWACPLRAGDNEK
                     VCYMAHNETAVPGAEAFSADEPIGQFVFHTTLPGAIRSLDDAELRILLIALLQEWRGR
                     ADGVQRGA"
     gene            7031..7774
                     /locus_tag="VGM72_14470"
     CDS             7031..7774
                     /locus_tag="VGM72_14470"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4266518.1"
                     /translation="MATLSEASSISDIHILLVDDDGEIRAELSAYLGAHGFQVSTAAN
                     GAEAERHLMEHRFDIMLLDLWLGSENGFDVLRRLRQLGRTPCIMVTAQDEITDKIVGL
                     ELGADDYMVKPVNLRELLARIRALIRRSGPPDEPGNQPMPPAGNDSEPNWKFDPMRRS
                     LSAPDGTQVPLTTAECDLLIELVSKEGLPQTRDELCRRVFNREWSPYDRSLDGIIVKL
                     RRKLEPNPDNPLVIKTVRGRGYVFTGFVR"
     gene            complement(7755..9539)
                     /locus_tag="VGM72_14475"
     CDS             complement(7755..9539)
                     /locus_tag="VGM72_14475"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEY4266519.1"
                     /translation="MLTNARERRRFRAKVSYLARQRAATFARVEMVGKSMKLLAGFTV
                     LLLTGTAALAQSAPPRLSVPDAGHVLTPPLALPAPGPTGPVLPQQPDLPAAAQTHLRF
                     TQVQIVGAVHLPEAKIAAAFDAFKGKKISGAELKVALDKVNALYAEAGYPLGRAFVPA
                     QKIKNGILTVRVVEGYIESIAVNADSEKTKALVERMAARLTAEKPLTRETLEQVILSI
                     QDLPGITLGSKFEAMNPETGGTRLVLAAQVRWVTVGLSLDNRANLQSLPFQPYATVTL
                     NNLLGQGDRISLTALLSPRQKDYAFYDLGYSQMIGSDGLNAGLDAAWAQTLDDVSLRP
                     FEVRSRVTRLSTQARYPLKRGTDENINLTGNLYYAGADYSLSHTSVGTFANDRNLALQ
                     LGTDYMRIFSPAFGVTGNAFVTQGLAGFAEEPHTRLHTIDRFTKLRAEGHAAWQPFEN
                     MTLKFGAMGQYSPNSLIASEEVAFGGLTFGRGFDTAEISGDEGIGFSFQPEYRIALGS
                     GFSITPYPLIDYAKAYNRRNDLQPNGELVSAGVGARFGMDNVATMTLELDKPINRTPF
                     GRRDRGWRVYAGFEIGVDGALSLIGQNL"
BASE COUNT         1945 a         2973 c         2792 g         1896 t
ORIGIN      
        1 gcgcggcgtg acgggcgaca cgatccagca ttccgcgctt ttggacgaga tcgcccgtgc
       61 catctccatc gttgtcggct cacggggtct ggcgctgaat acgcccgaac gctcccagct
      121 catcctggat gtcgtcaacg aaatcacggg gtttgggccc ctgcaaccgc tgctcgacga
      181 tcccacggtg gacgatgtga tcgtgaatgg gccgcggacc atttatgccg aacgcaatgg
      241 cgagctggag ccggtgacca cccgtttccg caatctcgat catttgatga atgtcatcca
      301 gcgcatcgtc gcgcccatcg ggcggcgcgt ggatgaatcc agcccgtatg tcgacggccg
      361 cttgccggat ggctcgcgca tcaatgtggt catcccgccg gtggcgctgg atggcccgca
      421 tatctcgatt cgaaaattcc gtgccgtccc cttcaccggc gaagacctgg tgcacaatgg
      481 cagcctgggc cccgaaggcc tgcgctttct gtccggcgcg gtgcgcgcac gccgcaatat
      541 tctgatctcc ggcggtacgg gctccggcaa aaccacgctt cttaatgtct tgagttccta
      601 tgtcgggaac aaggagcgta tcgtcaccat cgaagatacc gccgaacttc agctgcaaat
      661 ggcgcatgtg gtgcgcatgg aaacccgtgc ggcgaccgtg gacggcgcgc gcgaaatcac
      721 cgcgcgcgat ctggtccgca acgccctgcg catgcgtccc gaccggatca ttctgggcga
      781 agtgcgctcg cacgaggcga tcgaggtgct gcaagccatg ggaaccggcc atgacgggtc
      841 gatggcgacc attcacgcca acggcaccgc cgatgcattg gagcgattgg agatgctggt
      901 cggcctgcac ggtttttcca gcgaccttcg cgcggtacgc cggttcatcg tcagcgccgt
      961 cgatatcgtg gtacagacca tgcgtggcgc aggcggacgg cgcaccgtct ccagcgtggt
     1021 cgaaatcacg gggctggaag gatcccatta cgcccagcgt gaagtctatc gcggccccga
     1081 acaagtcgag cggaaggctg tggtgcgctg atgtcgataa ccggaaccat cgtcgcgctt
     1141 tcctgtgtcg ccttttccgg tttgctgctg gtgcggctcg ctgccgcgcg gtcgagactg
     1201 tcggacctgc gccggttgga caccgtcttg gcggcagcgc cgcacatggc ccaggacgca
     1261 aaatccgaaa gccgcctgcc gcccttcgtc tcaggtattc tggcacgcgc cggttgggaa
     1321 ccgacccggc gccaaattct ggctggagca accgcaacaa ttgccctcac cctggccgca
     1381 atggccgtct ccggcccgct cgcggctgga tcgactgcta taacgctcat ttttataagc
     1441 tttgcatttc tcgaatatcg cgctgtcgcc cggatcaaat tgctgtcgca atccatgctc
     1501 ggctttctcg agcgcatccg tcagctgctt tccgtgggca acagcctggc ggtagcactg
     1561 gagcgagcgg tggaaaattc tccgcccatc gtgggacaat gtctggcgcc caccctgcgg
     1621 cggatcgcca acggcagcgg cgtggctgaa agcctggagc gttgcgcaac tgaactcgat
     1681 ctctacgagc tgcacctcct ggcgaccgcg acgcgaacca atctgcgctt cggcggatcg
     1741 atgacagcca ttctgcgcaa catcatcgaa aatattcgca ggcgcacttc catcgaacgg
     1801 gagcttcggg ccaacacgac acaaatccgc gccagcgcat gggtgctcgc ccttttgccg
     1861 atcctggtcg caactctggt gatgtggacc aaccgggatt atgcgcgctg gtttctatcg
     1921 accgaaaaag gccacagcat gatcctttat gccgtcattt cccaaatcat gggcatcggc
     1981 tgcatgcgcc tggtaatacg gactcgatac tgacatggcc ccgcttctga catggacgct
     2041 ctgtattgcg tgcggtttga ccggcgggat cgcgctcgtt cacagcctgc gggattatgc
     2101 tctggatcaa tccgtggcga gacgctttcg cgagcatgtt tcgcccagct tgccggcgcc
     2161 aggaatgcga gcgcaactcc gtactctgtt ggagcgattt ggccgaagga ttgctggtac
     2221 ggattccatc cgccagcttc gggatgcgct gctgcaagcc ggctactttg cgccgggtgc
     2281 ggctttccta ttcgccgctt tacggttgat tgcgaccctg actgcggctt ccgtcctgct
     2341 tgcccgtccc tttctcaaat acgggacgat cgcttccggc gacgccgcga tcagtttctt
     2401 tttcggcttc ttcatttatc gcggctttac gatctttctc aaactgcgca gcgagagccg
     2461 ccagcgggac gtccgcaggg aactgcccta tgttctggac ttgctgttga tggttctcga
     2521 cagcggcgtc agcatcgacc aggcccttca gcatgtcagt cagcagatcg ggcgcgtcgc
     2581 ccctctcacc gcccatatgc tctcgcaata tatcgccgac acggacgatg ggatgcctta
     2641 tgacaaagcg ctcgaccggt tggcgcagcg catggcgatc agcgaaggac gcgatttcgc
     2701 aggcctgctg aagcaaaacc tgtttcaagg cggagaactg ggccagccgc tgcgccgtct
     2761 cgccgcggac atcagcgagg ccaggctgtc caatgcgcgc gaacaaaccg ggcgcaaagc
     2821 ggtgctgctg accctcatca tgctcgcctt tttcatgccg gtcctcatga tcgcgattgc
     2881 cgcccccgcc gtcactgatg ttctgggcac gttgcaacat atcgcgagcg atatgcaggg
     2941 gaaaaagaaa tgaaagcagg atttttgatt ttcactctgt tcctgaccgg atgcgccgcc
     3001 gcgccgcacg catccgggcc tgcgcggccg cagcccgcgg atgtggcaat gacgcccccc
     3061 gcccaggcgg aactctatct tggcgtgatc gacggtctga tccggcagaa gcgctatgag
     3121 gcggccatcg cctttctcgc gaaataccag aagaccgaac cgctgacacc gcgttacaac
     3181 aagctcgcag gcgatgcctt gaccggcgcg cgccgtttcg acgaggcgat cgccgcctat
     3241 tgccataccc tcaaatccaa tttcgcgccg gaggcctata acggtatcgg ccgcgccctg
     3301 tccgcacgcg ggcaatggcc ggaagcggcc gagaattttc gccaggccgc catactcgat
     3361 ccgtccaatg cggggtattt gaacaacttc gcgtatgccc agatgaagca gaatttccag
     3421 ggcgccgatc tggcgccggt ggtcagcgag ctgcaacgcg cccatgaact cgaccccgcc
     3481 tcgcctttaa tccgcaccaa cctggctctg gcgctgtcgc tttccggttc gcgcgaccaa
     3541 tttcttgctt ttctgaatac cattcccgac gccgccaaac gcaaacaggt cgcggatttt
     3601 tccacagcct ggaaggcgga ttggacgggc gacaccggca tcaaggaaag cacgccatga
     3661 cgcgcatcgc tttcttgctg tgtggcgtgg cccttaccgg cctgagcggt tgcgcttcat
     3721 ccgccgtgcg gccttccccc accacggacc aagtgatggc ggagcaatat cgcgcgcaag
     3781 gcgagcatgg gagcatcagc gggcccgaaa cagccgctat cgccgacgcc tatcggaaag
     3841 ccatcggcaa aagcacacaa gctgcgccga atagcgaaat gcgcaacgac gtcccgattg
     3901 aaaacagccc ttgacacaga ggagtgagta ccatgcgtga cactatcaaa aacctgactt
     3961 gccgatttct tgccgacgag agcgggctgt cggctgtcga atatggcttg ctggcggccg
     4021 ggatcgcagt cggtctctgg accctaatat ccggaatggg gacctcgttg cgggcaatct
     4081 atacaacggt gcaaggcaac ctttccgaag cgacgcctta aacacgcaac tgacgaacga
     4141 tgcgatcaat ttcgcgaatg gaagatttct gccaccggtt ccggcgaagt cgctgcgcga
     4201 ccacggcgat tgaattcgcc attgtcgcgc cgttcttgtt cgccatcatg ttcgggatca
     4261 tcggcttcgg catgcaattc gccacgcaca tcgcactcac ttatgccgca accgaaggcg
     4321 ggcgcgctgc tgtggccgga ctgaatgacg gcgagcggga gactttggcg cgcggggcca
     4381 tcaccaaggc cctcacagcc ttgaaaccgc tgatcaaagt gggcaacgcg accatcagct
     4441 tcgatatgac cgccgaagcc accgaccaga agatcaatat cgggatcgcc tacaacgata
     4501 cccgcttcgc cgtgttgccc tttgtaccga acttcaacaa tcaggcaccc gttacggtta
     4561 catactatgt caccgatcct tccggctgaa cccgcacggc gagacgccgc aaccggacaa
     4621 ggcattttgc ctggacttcg ccgcgtgctg agcgcgacct ctgtgcaagc ctgtcacaaa
     4681 ggattggccg acgccgcgca gttgctggat gcagaattcg gcttctccac tctggcagtg
     4741 gctgaaatcg cctccacgcc gcagggacat gccgttgtat ggcaaaccga cggtcccttg
     4801 cgcggctccg gccccctatt gcgtgcttgg ctcgagcgcg ccagccgcaa tccgggtcgc
     4861 agcctggcgt ttggtgatgc cgccacagga ctactcatgg ctggagcgcc ggaaggcgat
     4921 gcccccatgg tcgcgttgat cgcggatggc acgctgctgc ctccaccact ggaaagcgcc
     4981 atgcgcgacc tggcggagtt ttcagcgcgc cacctttgtc atttgcgacg ggaaagcgaa
     5041 attcttcgcc gccggcaaag gttggatata gacggggccc gcgatacggc actgctccgc
     5101 gccaacgccg atttgatctg ggaggccgac agcgatggca ttctgcatgt cacgcatatc
     5161 ttccaggatc gccgcgatct ggcgaaattg tttgaaggcc gcgtccttag cgacatcggt
     5221 gttggcgcca gcgacctcca gagtgccgca aaatccggtc aggtgctgcg ctcgctaccc
     5281 gttactgtaa ctggtgcgag cgggcctata tttctcacag cttgccccac ccttgggcct
     5341 tccgcgccat tccccttgcg cggcacgatt gcgacggcgc tcgagcaagc aagagaaaga
     5401 ttggtgatcg acacccatac cctcgagacc atcctggcat cacggcaccg cgaagagcag
     5461 ctgcgccggg agaccgaaac catgatgatg ggcttgcgga ttttgttggg cgactctccc
     5521 ttccgcgaaa aattggagca attggtaagg cggctggcgg acgcgatcga tggcgacgaa
     5581 gtccgcctcg ttctgttccg cccgggcgaa gcacctcgtc ttgtactgcc ggaaggtcga
     5641 ggagaagaga attccgcact gaccgatatt gcctccttgg ccgggcagcg cacggtcacc
     5701 ctccttcgcc aaactggtca cgacaatgcg cgtatatgca aagccttggc catgcggaac
     5761 ggtgacatcg ttctggtcgc gttgccggcc gcggcagagc gctattatct cctatgccgc
     5821 acaaggcgta acctcaattc cgacgatcac ggcctcgccg aacgggtttc gcttctgttg
     5881 caacaggcaa ttcttctgca ggccgatcaa aagcaaattc tgcattccgc taaattgtca
     5941 gcattgggcc agatgtcgac cggcatcgca catgaattgc gccagccgct taatgccatt
     6001 tcaatcgcgg cgcaaaatat cgacctgatg gtcggtctgg gaaaagccgg tccggaattc
     6061 ctacaagaaa aaaccgaacg aatcctggcg cagatcgacc gcgcctgcaa agtcatggac
     6121 cggatgcgcc gcttcggcag gaaaacagtg ggtgagtaca agccggcatc ggccgccgcc
     6181 atagcgcgca gcgctcgttc tctgatggac cctgtcataa tctcgtccgg cattgcggtc
     6241 gatatcgaag tccccgatga cttagaggtc ttggcggacg agttggagat cgaacaggtc
     6301 ctcgtgaatc tgattcagaa tgcgtccgat gccatgacag gccgggcggg tggaagaatt
     6361 cgcatatggg cacgagacga tttcgccgat cccagcgtcg ttaaattgga agtcgaagac
     6421 aatggctcgg gatttccgcc ggaaattctc aaacacgcgc tagacgcatt cttcacgacc
     6481 aaaccggaag gcaaaggcac gggcctcggc ctctctatcg cgcacgcaat cttgcgcgag
     6541 catggcggac gactgctggt cagcaatagc gaggccggcg gcgggttgat tacgcttatt
     6601 ttacgacgcc ccgaagcaag aattctatct tttgccccgc gacccgtcac ggcgggggta
     6661 ccatgacaaa aaaagctgca atgtctcccc ttaccgcgat caggctccgc gctgaacccc
     6721 gtcagcacga ccgcgccatt cctgaagcaa cgcgatcaga agaattcgca actcggcatc
     6781 atcgagagag cggatcgcgc ccggcaatgt cgtgtgaaac acaaactgac caattggctc
     6841 gtcggcagaa aacgcttcag cgccgggaac cgcagtttca ttatgagcca tatagcagac
     6901 cttttcgttg tccccagccc gaagcggaca ggcccagtat ggcccagcca gggtccaaac
     6961 aattgtcgag atatgtttca ttgcgtatgg gcgctcccag gcgccccaac ttcccttgag
     7021 agaaacgccc atggccacgc tcagcgaagc cagttctatt agcgacatcc acatcctttt
     7081 ggtggatgac gatggcgaga tccgcgccga actgagcgcc tatttgggag cccacggctt
     7141 tcaagtctcc acagctgcaa acggcgcgga agccgaacgg catttgatgg agcatagatt
     7201 cgatatcatg ctgctggatc tatggctggg aagcgaaaat ggcttcgacg tgctgcggcg
     7261 cttgcgtcag ctgggcagaa caccctgcat catggtcacg gcccaagatg agatcacgga
     7321 caagattgtg ggccttgagc ttggcgccga cgattatatg gtcaagccgg tcaatctgcg
     7381 cgagcttctg gcccgtatcc gcgctctgat ccgccgcagt ggcccgccgg atgagcctgg
     7441 aaatcagccc atgccgccgg caggcaatga ctccgagccc aactggaaat tcgatcccat
     7501 gcggcgcagc cttagtgcgc ccgacggcac acaggtcccc ctcaccacgg ccgaatgcga
     7561 ccttctgatc gaactggttt ccaaggaagg actgccgcaa acccgggacg aactctgccg
     7621 ccgcgttttt aatcgcgaat ggagtccata cgaccgcagc ctggacggga tcatcgtcaa
     7681 gcttcggcgc aagctggagc ccaatcccga caaccccctg gtaatcaaga ccgtgcgcgg
     7741 gcgcggttat gtcttcacag gttttgtccg ataagcgaca gcgcaccgtc gacgccaatt
     7801 tcaaagcctg cataaacgcg ccagccgcga tcgcgccggc cgaaaggcgt gcggttgatt
     7861 ggcttatcca gttccagggt catggtggca acattgtcca ttccgaagcg ggcgccaaca
     7921 ccggccgaaa cgagttcgcc attgggttgc agatcgttac gccggttgta ggccttggca
     7981 taatcgatca acggataggg cgtgatactg aagccggatc ccagcgcgat gcgatattcg
     8041 ggttggaaag aaaagccgat gccttcatcg ccggaaattt cggcggtatc gaagccgcgt
     8101 ccgaatgtga gcccgccgaa cgccacctct tccgaggcaa tgagggaatt ggggctgtat
     8161 tggcccatcg ccccgaactt aagggtcata ttttcaaaag gctgccaggc ggcatgccct
     8221 tcggcacgaa gttttgtgaa gcggtcgatg gtatgcaaac gcgtatgcgg ctcttcggca
     8281 aagccagcca gcccctgcgt gacaaaagca ttgccggtga cgccgaaagc cggcgagaaa
     8341 atccgcatgt agtcggtgcc cagttgcagc gccagattgc ggtcattggc gaaggtgccg
     8401 accgatgtgt gcgacaggct gtaatcggct cccgcataat aaagattgcc ggtgagattg
     8461 atattctcgt ccgtgccgcg cttcaaagga tagcgcgcct gagtcgaaag ccgtgtcacg
     8521 cggctacgca cttcgaaagg ccgtaggcta acatcatcga gcgtttgagc ccaggccgcg
     8581 tccagaccgg cattgaggcc gtcactgccg atcatctggg aataacccag atcgtagaaa
     8641 gcatagtcct tctgccgcgg cgacaacaac gccgtcaacg agatgcggtc gccctgcccc
     8701 agaagattgt tcaaagtcac ggtggcatag ggctggaagg gcagagattg cagattggcg
     8761 cgattgtcca ggctgagacc caccgtcacc cagcgcacct gcgcggccag aaccagccgt
     8821 gtgccgccgg tctccggatt cattgcttcg aactttgaac ccagcgtaat gccgggcaga
     8881 tcctgtatcg acagaatgac ctgctccaga gtttcccgcg tcaaaggctt ttcggcggtc
     8941 aggcgcgcgg ccatgcgctc gaccaaggct ttggttttct cgctgtccgc attcaccgcg
     9001 atgctttcga tatagccttc gaccacccga acagtgagaa tgccattctt gattttttgc
     9061 gccggcacga aagcgcggcc cagaggatag cccgcctccg cgtagagcgc gttgaccttg
     9121 tccaacgcga ccttcagctc cgcgccggaa atctttttgc ctttgaacgc gtcaaaagcc
     9181 gcggcgatct tcgcttcagg cagatgcaca gcgcctacga tctgcacctg ggtgaagcgc
     9241 agatgggtct gagccgcggc tggcagatcg ggctgctgcg gcagcaccgg accggttggt
     9301 cccggcgccg gcaacgccag cggcggtgtc agaacatgcc ccgcatccgg cacagacaga
     9361 cgcggcggcg cgctttgcgc caatgccgcc gttcctgtca aaagcagtac agtgaatcct
     9421 gccaggagtt tcatcgattt gcccaccatt tccacgcggg caaaagtggc agcgcgctgg
     9481 cgcgcgagat acgagacttt ggcgcgaaaa cgtcgccgtt cgcgagcgtt tgtaagcatc
     9541 tgtgagacgc gcaaaaaaga acccaggcgc tttcgcacct gggttctctt gccgccgaat
     9601 acgccg
//
LOCUS       DASYEG010000346         6188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1737852, whole genome shotgun sequence.
ACCESSION   DASYEG010000346 DASYEG010000000
VERSION     DASYEG010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6188
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1737852"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /gene="nusB"
                     /locus_tag="VGM72_14480"
     CDS             complement(<1..401)
                     /gene="nusB"
                     /locus_tag="VGM72_14480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY4266520.1"
                     /translation="MNGTAADAHETRSAARLAALQALYQLEMTGNAPGDVVAEFIQHR
                     FGRDSETGEGVPHDEAFFSDIVHGVLKHQVEIDRSVARSLASGWTLSRIDSILRALLR
                     SATYELVARRDVPAKVVIDEYVELARDFFEG"
     gene            complement(398..817)
                     /gene="ribH"
                     /locus_tag="VGM72_14485"
     CDS             complement(398..817)
                     /gene="ribH"
                     /locus_tag="VGM72_14485"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEY4266521.1"
                     /translation="MTRVLIVEARFYPEISDALYAGAARALEAAGVEYERVNVPGALE
                     IPAAIKFAGAHYDGFVALGCVIRGETYHFEIVANESARGLMDLILRDGLCIGNGILTV
                     EDEEQAFVRAHTAEGDKGGDAARACLALIALKTRFRP"
     gene            complement(814..1941)
                     /gene="ribB"
                     /locus_tag="VGM72_14490"
     CDS             complement(814..1941)
                     /gene="ribB"
                     /locus_tag="VGM72_14490"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEY4266522.1"
                     /translation="MNIFHDYISELPDVIDDARNGRMFILVDAEDRENEGDLVIPAQM
                     CTPQAINFMARFGRGLICLALTQERADQLQLPMMAVENRTRNQTAFTVSIEAKEGIST
                     GISAHDRAHTVSVAIDPTKGPSDIVMPGHVFPLVARHGGVLVRTGHTEAAVDIARLAG
                     LYPAGVICEIMNDDGTMARLPDLVKFAQLHGLKVGTIADLISYRRKHDHFVKRIKEMD
                     FESRNGGHFKLMFYLNTIENQEHVALVKGDLSGPEPPLVRMHAYNMFDDTLAAKWGPG
                     DILGESMRMIGKAGRGVVVLIRRHRIAPISEVLAQSTKTMPDMEQSRLKEYGVGAQIL
                     LDLGVENMVLLSNATGRRVVALEGYGLKIVETRSVVPGTVK"
     gene            complement(1938..2543)
                     /locus_tag="VGM72_14495"
     CDS             complement(1938..2543)
                     /locus_tag="VGM72_14495"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEY4266523.1"
                     /translation="MFTGIITDVGQVRHVEKRGDTQIVIATHYDVDAIDIGASIACSG
                     VCLTVVDKGNAKDRWFAVTASGETLSKTTIANWKPGDPVNLERPMRLGDELGGHIVAG
                     HVDGVAEIVGVVPEGESHRFTFLAPPSLAPFIASKGSVTVDGVSLTVNEVEGARFGVN
                     IIPHTRKVTTFGAAKSGGTVNLEVDLLARYVARLAQVTKLP"
     gene            complement(2553..3656)
                     /gene="ribD"
                     /locus_tag="VGM72_14500"
     CDS             complement(2553..3656)
                     /gene="ribD"
                     /locus_tag="VGM72_14500"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEY4266524.1"
                     /translation="MTSQHDDLVHMRHALRLAERGLGDVAPNPAVGCVIVSTDGHIVG
                     RGWTQPGGRPHAETVALAEAKERARGATAYVTLEPCAHHGATPPCADALVQAQIARVV
                     GAVRDPDSRVAGKGFARLEAAGVAVTLGVLEAQAAALNLGFFKRVTEGRPLVALKIAQ
                     SLDGYVADANGNSKWITGERARAHGHLLRGKYDAILVGIGTVLADDPALTSRLPGLAR
                     RSPRRIVLDSRLRLSPGSQLARTAREHPVIVFTTANGGGEALSALGVHIERVDRDAAG
                     RPDVAAVLQALAKRGLTRLLVEGGPSVHAAFLERGLVDMLHIYGAPLLIGGGGRPAVA
                     PFGTSDLNAARRFTSIERRTLGPDVLETFTLTV"
     gene            complement(3678..4139)
                     /gene="nrdR"
                     /locus_tag="VGM72_14505"
     CDS             complement(3678..4139)
                     /gene="nrdR"
                     /locus_tag="VGM72_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEY4266525.1"
                     /translation="MRCPFCGHDDTQVKDSRPSDDNAAIRRRRLCPGCGGRFTTFERV
                     QLRELTVLKKAGRREPFDREKLERAIHHALRKRPVEAERVERMITGIVRRLESLGENE
                     IQSSMIGELVMQALASLDKVAYIRFASVYKNFREAKDFETFASELSGVDRD"
     gene            complement(4181..5428)
                     /gene="glyA"
                     /locus_tag="VGM72_14510"
     CDS             complement(4181..5428)
                     /gene="glyA"
                     /locus_tag="VGM72_14510"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY4266526.1"
                     /translation="MAATDPEVASAIAKELKRQQDKIELIASENIVSRAVLEAQGSVL
                     TNKYAEGYPGRRYYGGCEYVDIAETLAIERVCRLFQCDYANVQSHSGAQANQAVFFAL
                     LQPGDIFMGLNLASGGHLTHGSPVNMSGKWFKPVPYNVRRQDHRIDMDEVAALALAHK
                     PRLIVAGGSAYPRTIDFAAFRKIADSVGAYLMVDMAHFAGLVAGGVHPNPLDHAHVVT
                     TTTHKTLRGPRGGVILSRDPELGKKFNSAVFPGLQGGPLMHVIAAKAVAFGEALKPEF
                     KLYAKNVVENAKALAETLQSRGLDIVSGGTDTHLMLVDLRPKKVTGRAAEKALDRANM
                     TCNKNAIPFDTEKPAITSGIRLGSPAGTTRGFGAAEFRDIGNMIAEVIDGLAAKGEEG
                     DPSVEESVKNRVLDLCRRYPIYS"
     gene            5881..>6188
                     /locus_tag="VGM72_14515"
     CDS             5881..>6188
                     /locus_tag="VGM72_14515"
                     /inference="COORDINATES: protein motif:HMM:NF017277.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HEY4266527.1"
                     /translation="MAQNSDNQSLRSDDILKLTTEIVAAYVSNNPIPFSEVPAMIKSV
                     HSTLGGLGGGTQNEIQTSQKPAVAVKRSVNPDYIICLEDGKKLKMLKRYLRSNYNLTP
                     "
BASE COUNT         1240 a         1935 c         1817 g         1196 t
ORIGIN      
        1 tcgccttcga agaagtctcg ggcaagttcg acatattcgt cgatcaccac cttggccggc
       61 acatcgcgcc gcgccaccaa ctcataggtc gcgcttcgta aaagcgcccg caaaatcgaa
      121 tcgatccggc tcagcgtcca gcccgaggcg agcgaacgcg caaccgagcg gtcgatctcg
      181 acctgatgct ttaggacacc atgcacgata tcggagaaga acgcttcatc atggggtacg
      241 ccctctccgg tctcggaatc gcgcccaaaa cgatgctgaa tgaattcagc gacgacatcg
      301 ccgggcgcgt tgcccgtcat ctccagctga tacagcgcct gcaaagccgc cagccgcgcg
      361 gcgctgcggg tttcatgagc gtcagccgcc gtaccgttca tggccggaaa cgcgttttga
      421 gcgcgatcag cgccaggcac gcgcgcgccg cgtcgccgcc tttgtcacct tcggccgtat
      481 gggcgcgcac gaatgcctgc tcttcgtctt ccaccgtgag aattccattg ccgatgcaaa
      541 gaccgtctcg aaggattaga tccatcagcc cacgcgcgga ctcattcgcc acgatctcga
      601 aatgatacgt ctcgccgcga atgacgcagc ccagcgcgac gaagccgtca taatgggcgc
      661 cggcgaattt gatggccgcc ggaatttcaa gcgcgcccgg aacgttcacg cgctcgtatt
      721 ccacacctgc cgcctcaagt gcgcgcgcgg cacccgcata gagcgcatcc gaaatttccg
      781 gatagaaacg cgcttcgacg atcagtacgc gcgtcatttc accgttcccg gcacgacaga
      841 cctcgtctcc acgatcttca gcccataacc ttcgagcgcg accacccggc ggccggtggc
      901 attgctgagc aagaccatgt tttccacacc gaggtcgagc aggatctgcg ctcccacccc
      961 atattccttc agccgcgact gttccatgtc gggcatggtt ttggtcgatt gcgccagcac
     1021 ctcggagata ggcgcaatgc ggtgccggcg aatgagcacg acgacacccc tccccgcttt
     1081 gccgatcatg cgcatggact cgcccagaat atcgcccggt ccccatttcg cagccagcgt
     1141 gtcgtcgaac atattgtagg catgcatgcg cacgagcggc ggttcggggc cgctgagatc
     1201 acccttgacc agcgccacat gctcctggtt ctcgatggtg ttgagataga acatcagctt
     1261 gaaatgcccg ccgttgcggc tttcgaaatc catttccttg atgcgcttca cgaaatgatc
     1321 gtgtttgcgg cgataggaga taagatcggc aatggtgccg actttcagcc catgcaattg
     1381 cgcgaatttc accaggtccg gtagccgtgc catggtgccg tcgtcgttca tgatctcgca
     1441 gatgacgccg gccggataaa gtcccgcgag ccgcgcgata tcgacggcgg cttcggtatg
     1501 gccggtccgc accaatacgc cgccatgacg cgcgaccagc gggaagacat ggcccggcat
     1561 cacaatgtcg ctcggcccct tggtgggatc gatcgcgacc gaaaccgtgt gcgcgcgatc
     1621 atgcgcggag atgccggtgg aaatgccttc ctttgcttcg atggaaacgg tgaaagccgt
     1681 ctggttgcgc gtgcggtttt cgactgccat catcggaagc tggagctgat cggcgcgttc
     1741 ctgcgtcaag gcaaggcaga tgagaccacg cccgaagcgg gccatgaaat tgatcgcctg
     1801 cggcgtgcac atctgcgcgg gaatgacgag gtcaccttca ttctcccggt cttcggcatc
     1861 caccaggatg aacatgcgtc cattgcgcgc atcatcgatg acgtcgggca attcggaaat
     1921 gtagtcgtgg aaaatgttca tggaagcttt gtgacttggg ccagccgcgc gacgtaacgc
     1981 gccaggagat cgacttcaag attgaccgtg cccccggatt tggccgcgcc gaatgttgtg
     2041 accttccttg tatggggaat gatattgacg ccgaagcgcg ccccctccac ctcgttgacg
     2101 gtcaaggaaa ccccgtccac agtcacggat cccttggagg cgatgaaagg cgcgaggcta
     2161 ggcggcgcga gaaacgtgaa gcggtgggat tcgccttcgg gcacgacccc cacgatctcg
     2221 gccacaccat ccacatgacc ggcgacgata tggccgccca attcgtcgcc cagccgcatc
     2281 ggccgttcca ggttcaccgg gtcgccaggt ttccagttcg caatcgtggt cttcgacagg
     2341 gtttcacccg acgccgtcac cgcgaaccag cgatccttgg cattgccctt gtccaccacg
     2401 gtaagacaga cgcccgagca ggcgatggaa gccccgatat cgatggcgtc cacatcataa
     2461 tgggtggcga tgacgatttg ggtatcgccc cgcttctcga catgccggac ctggccgacg
     2521 tcggtgatga tgcctgtgaa catgaggatg ttctagactg tgagggtgaa ggtttccagg
     2581 acgtcggggc cgagcgtccg ccgttcgatc gatgtgaagc ggcgagcagc attcaaatcg
     2641 gacgtcccaa agggtgcaac ggcagggcgc ccgcccccgc cgatcaacag cggcgccccg
     2701 tatatatgaa gcatgtccac caatccacgt tccaggaatg cggcatgcac gctggggccg
     2761 ccttccacca gaaggcgcgt caggccccgc ttggccagcg cttggagcac agcagcgaca
     2821 tcgggacggc cggcggcatc gcgatccaca cgctcgatat gcacccccaa cgcggacaag
     2881 gcttcgccgc cgccgtttgc ggttgtgaac acaatcaccg gatgctcgcg cgccgtgcgg
     2941 gccaattggg agccgggcga aaggcgcaga cgcgaatcca gcacaattcg ccgcggcgaa
     3001 cgtcttgcga ggcccggcag gcggctggtg agcgccggat cgtcggccag cacggttccg
     3061 atgccaacga gaatcgcgtc gtatttccca cgcagcaaat ggccatgggc gcgggcgcgc
     3121 tcccccgtga tccatttgct gttgccgttt gcgtcggcga cataaccatc gaggctctgg
     3181 gcgatcttca gcgcgaccag aggccgaccc tccgtcacgc gtttgaagaa gccaagattg
     3241 agcgcggccg cttgcgcttc cagaacgccc agcgtcaccg ccacgccagc cgcttccagc
     3301 cgcgcaaatc ctttgcccgc gacgcgggaa tcgggatcgc gcacggcgcc caccacgcgt
     3361 gcgatttgcg cttgaaccag cgcgtccgcg caaggcggtg tggcgccatg atgcgcgcag
     3421 ggttcgagcg tgacataggc ggtggcgccg cgcgcccgtt ctttggcctc agccagcgca
     3481 acggtttcgg cgtgcggcct gccgccgggt tgtgtccaac cgcgtcccac gatgtggcca
     3541 tcggtggaaa caatgacgca gccaaccgcc ggattgggcg ctacatcgcc caggccgcgt
     3601 tctgccaggc gcagcgcgtg gcgcatgtgg acaaggtcgt catgctgcga ggtcaagttt
     3661 tctcagagtc cagcacatca gtcccgatcg acaccggaaa gctcgctggc gaaggtctcg
     3721 aaatccttcg cctcgcggaa attcttgtac accgaggcaa accggatata ggcgactttg
     3781 tccagagagg cgagcgcctg catcaccagt tcgccgatca tgctggattg gatttcgttc
     3841 tcacccaggc tctccaggcg ccgcacgatt ccggtgatca tccgttccac gcgctccgcc
     3901 tccacggggc gtttgcgcaa cgcatgatgg atcgcgcgct ccagtttctc gcggtcgaaa
     3961 ggctcgcgcc gtccggcctt tttgaggacc gtcaactcgc gaagctgcac gcgctcgaag
     4021 gtggtgaagc gtccgccgca gcccgggcac aagcgccggc ggcggatggc ggcgttgtcg
     4081 tccgagggac ggctgtcctt cacctgggtg tcgtcatgtc cacagaacgg gcaacgcatg
     4141 ggagaaaatg cctttcttcg tcacgaatag attggcttcg tcacgaatag attggatagc
     4201 ggcggcagag atcgagcacg cgatttttca cgctttcctc gaccgacgga tcgccctctt
     4261 cacccttggc ggcaaggcca tcgatgactt cggcgatcat gttgccgata tcgcggaatt
     4321 cggccgcgcc gaaaccgcgc gtggtgcccg caggcgagcc gaggcggatg cccgaggtga
     4381 tcgcgggctt ttcggtatcg aagggaatcg cattcttgtt gcaggtcata ttggcgcgat
     4441 cgagcgcttt ttccgcggca cggcccgtca cttttttcgg gcgcagatcg accagcatca
     4501 aatgcgtatc ggtgccgccc gatacaatgt cgagtccgcg cgactgaagc gtttcggcca
     4561 gcgctttcgc gttttcgacg acattcttgg cgtaaagctt gaactccggc ttcaacgcct
     4621 caccgaacgc gaccgccttg gcggcgatga catgcatcaa ggggccgccc tgcaggccgg
     4681 gaaagacggc cgagttgaat ttcttcccaa gctcgggatc gcgcgacagg atcacgccgc
     4741 cgcgcgggcc ccggagcgtc ttgtgcgtgg tcgtggtgac gacatgggcg tgatccagcg
     4801 gattggggtg cacgccgccc gccaccaggc cggcaaaatg tgccatatcg accatcagat
     4861 aggcgccgac cgaatcggcg attttgcgaa acgcggcgaa atcgatggtg cgcggatagg
     4921 ccgagccgcc ggcgacgatc agcctgggtt tatgcgcgag cgccagcgcc gccacctcgt
     4981 ccatatcgat acgatggtcc tgacggcgta cattgtacgg caccggcttg aaccatttgc
     5041 ctgacatgtt caccggcgat ccgtgcgtga ggtgtccgcc cgatgcgaga ttcaacccca
     5101 tgaaaatatc gccgggttgc agcagggcga agaataccgc ctgattcgcc tgcgcgccgg
     5161 aatgggactg cacattggcg taatcgcact ggaacaaacg gcacacccgc tcgatggcga
     5221 gcgtctcagc gatgtcgaca tattcgcagc cgccgtaata gcggcggcca gggtaaccct
     5281 cggcgtattt gttggtcaga accgagcctt gcgcctccag gacggcgcgg ctgacgatgt
     5341 tttcggatgc gatcagctcg atcttatcct gctgacgttt cagctccttg gcgatcgccg
     5401 atgcgacctc cggatccgtg gcggccacgc cgcgtccaaa aaaattctca ctcaccgcaa
     5461 ccatgcccgc tcctcggtaa cgccgatcgt gttgtgcacc tttcgggcac ctttaccaaa
     5521 tcttggggcg atgcacgatc aatcgcggga ccgcctcaac aggaatcttt ccatggggtt
     5581 tggatggaaa actccgatcc tccgatgcct atagcatagg caagacagag ctaaagaccg
     5641 cctcaatggg tattccaaag acttcaagcg ccgcattggg tgcgcaaggc aagttcttga
     5701 ggccacggaa acagcgcacc atcatgattt gcttatgaag gattcgggca ccacccaaat
     5761 tgttcgaaag ccggcgtcag ctttccaatg cccatgtgtg gtcttgattt ctcttttgtg
     5821 tcaggtgcat aaaagcaaca ctcaagcatt ggtactgtct agctcttgga gttttcagaa
     5881 atggcgcaaa attcggataa ccaatctcta cgatccgacg atatcctcaa attgacgaca
     5941 gagatcgttg ccgcctatgt cagcaacaat cccattccgt tttccgaagt ccccgccatg
     6001 atcaaaagcg tacatagcac gctcggtgga ttgggtggcg gcacccaaaa tgaaatccag
     6061 acaagccaga aacctgcggt ggccgtcaaa cgttccgtca atcccgacta catcatctgt
     6121 ctggaagacg gaaagaaatt gaaaatgctg aaacgctatt tgcgctccaa ctacaatttg
     6181 acgccgga
//
LOCUS       DASYEG010000347         2976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_3908435, whole genome shotgun sequence.
ACCESSION   DASYEG010000347 DASYEG010000000
VERSION     DASYEG010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3908435"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VGM72_14520"
     CDS             <1..451
                     /locus_tag="VGM72_14520"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY4266528.1"
                     /translation="GQPLALGKGRILREGSSVAILNFGARLAECLKAAERFSAYGLSA
                     TVADARFAKPLDTDLVRRLAQNHEVLLTVEEGAVGGFGAHVLHFAAWDGLLDRGLKIR
                     TMILPDRFLDQDTPERMYEISGLDAKGIVAAGLSALGRERDAAAVRG"
     gene            451..1203
                     /locus_tag="VGM72_14525"
     CDS             451..1203
                     /locus_tag="VGM72_14525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEY4266529.1"
                     /translation="MPDPSERADLFLVSRGFAATRSEAQGAIRSGNVTADGQTVSKPA
                     QKLQEGMNILYQPAHAYVSRGGVKLAAALDHFALSPEGRVCLDVGASTGGFTQVLLER
                     GAARVYAIDVGHGQLHAKLASDARVTRIDGLNARDLDTTKIPEPPRAITADVSFISLK
                     VALTPALHLASADSWAVLLVKPQFEVGPRGVSRGGIVKDDGLREGALAGIAAWIVAAG
                     WQVLGTMDSPIAGGDGNREYLLAARRAEAMRK"
     gene            1282..2481
                     /locus_tag="VGM72_14530"
     CDS             1282..2481
                     /locus_tag="VGM72_14530"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4266530.1"
                     /translation="MMMNRRQFAYGLTCAAGTLGFARATGAFAAQGRYDLIVKGGRVI
                     DPSLRLDGVRDIAISGGRIAAVEPNIAAGAAEMIDARGKLVVPGLIDIHTHAARTKEG
                     PGLVLQDGVTGWIDAGSQGADHIGDTIAVARSSPQQGRVLINIGRAGILPEGDTMDLA
                     RADVAAAKDAIAKNRDFIVGVKARLSRDVAGPNDNEVMRRTQEVASAFNLPVMIHMGQ
                     TISPLPLLINQLKKGDVVTHMFAPPPNSIIDENGRILPEVLAARRRGVWFDVGNGQAG
                     HMRWDTVAAIMKAGFWPDSFSTDWNTNAHMTGVIDLPNCMSKYFDYGMTVEQAVARAT
                     VNPSRMFALFNDRGTLNVGAQADVAVLELRAGDFEYLDNFKNTKPGKQRLLPSATILA
                     GKRVARA"
     gene            complement(2490..>2976)
                     /locus_tag="VGM72_14535"
     CDS             complement(2490..>2976)
                     /locus_tag="VGM72_14535"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HEY4266531.1"
                     /translation="WVYKNAASFGGDPNHIYLCGQSSGAHLSGVALITDWQKDFGLPA
                     DIVKGGVLLSGMYEMKPVRLSARSKYVKFDDAMEDSMSTIRHIDRLHTPITLLYGTNE
                     TPEFQRQSRDFAAAVKAAGKPVTLIVAPNHNHYELQETMANPYGWAGRAALAMMGLGK
                     P"
BASE COUNT          524 a          948 c          962 g          542 t
ORIGIN      
        1 cgggcagccg ctggcgctcg gcaagggccg catcctgcgg gagggaagct ccgtcgcgat
       61 tctgaatttc ggcgcgcgtc tcgcggaatg cctgaaggcc gccgaaagat tctccgccta
      121 cggattgtct gcgacagtgg ccgatgcccg cttcgccaag ccgctcgata ccgatctggt
      181 acggcggctg gcccaaaacc atgaagtgct gctgaccgtc gaagagggtg ccgttggcgg
      241 ctttggcgcg catgtgctgc atttcgcggc ctgggacggg cttctcgatc gcggcttgaa
      301 gattcgcacc atgatcctgc ccgaccgctt cctggatcag gacacgcccg agcggatgta
      361 cgaaatctcc gggcttgacg ccaagggcat cgttgccgcc ggactttcgg cgcttggacg
      421 cgaacgcgac gccgcggccg tgcggggata atgcccgacc caagcgagcg cgccgacctt
      481 ttcctcgtca gccgggggtt tgccgcgacg cgctccgaag cgcaaggcgc catccgctcg
      541 ggcaacgtga cggccgatgg tcagaccgtc tccaaaccgg cgcagaaact tcaagagggg
      601 atgaacattc tttaccagcc cgcgcatgcc tatgtgtccc gtggcggcgt gaagcttgcc
      661 gcggcgctgg accatttcgc actctcgccc gaggggcgcg tgtgcctcga tgtcggcgct
      721 tcgaccgggg gtttcacgca ggtgctgctg gaacgcggcg ccgcgcgcgt ctatgcgatc
      781 gatgtcgggc atggacaatt gcatgccaag ctcgcctccg atgcgcgcgt gactcggatc
      841 gatgggctga acgcgcgcga tctcgacacc acgaaaattc ccgaaccgcc acgcgcgatc
      901 accgccgatg tcagcttcat cagcctgaag gtggcgctga ccccggcgct gcatctggcg
      961 agcgcggatt cctgggcggt gcttctggtc aagccgcaat tcgaggttgg cccgcgcggt
     1021 gtgtcgcgcg gcggcatcgt caaggacgat ggcttgcgcg aaggggccct tgccggcatc
     1081 gccgcctgga tcgtggccgc cggctggcag gtgctgggca cgatggatag tcccatcgcg
     1141 ggcggcgacg gcaatcgcga atatctgctt gccgcgagac gcgccgaagc gatgcggaaa
     1201 taagcttcgg ccttttaccg gctgcgaaca aaaccgctcc cgctctatgc tataatacga
     1261 ccataagagt tcgggagggc gatgatgatg aaccggcgtc agtttgcata tggccttacc
     1321 tgtgccgcgg gcacgctcgg ttttgcgcgc gcgacgggcg cgttcgccgc gcaaggccgc
     1381 tatgatctca tcgtcaaagg cggacgcgtc atcgatccct cgctcaggct ggatggcgtg
     1441 cgcgacattg cgatttccgg cgggcgtatc gccgccgtgg agccgaatat cgcagccggc
     1501 gccgccgaga tgatcgatgc ccgcggcaag ctcgtcgttc cgggcttgat cgacatccac
     1561 acccacgccg cgcgcaccaa ggaaggtccg ggcctcgtcc tgcaggacgg tgtcacggga
     1621 tggatcgatg cgggctcgca gggcgccgat catatcggcg acaccatcgc ggtggcgcga
     1681 agctcaccgc agcagggccg ggtcctgatt aatatcgggc gcgccggcat cctgcccgaa
     1741 ggcgatacga tggacctcgc acgcgccgat gtcgccgcgg ctaaggacgc aatcgcgaag
     1801 aatcgcgact tcatcgtcgg ggtcaaggcg cggctgtcgc gcgatgtcgc cggccccaac
     1861 gacaatgaag tcatgcgccg gacgcaggaa gtggcgagcg ccttcaacct gcccgtgatg
     1921 atccatatgg gccagacgat ctcgccgctg ccgcttctga tcaatcagct caagaagggc
     1981 gacgtcgtca cgcatatgtt cgcgccgccc cccaacagca tcatcgacga gaacggccgc
     2041 atccttcccg aagtgctcgc cgcccggcgg cgtggtgtgt ggttcgacgt cggcaacgga
     2101 caggccggcc atatgcgatg ggacacagtc gcggccatca tgaaggcggg cttctggccg
     2161 gattccttct ccacggactg gaacaccaat gcccatatga cgggggttat cgaccttccc
     2221 aattgcatgt cgaaatattt cgactatggc atgaccgtcg agcaggccgt cgcgcgcgcc
     2281 accgtcaatc cctcgcgcat gtttgcgctg ttcaacgatc gcggcacgct gaatgtgggt
     2341 gcccaagccg atgttgcggt gctcgagctg cgtgccgggg atttcgaata tctcgacaat
     2401 ttcaagaaca cgaagccggg aaagcagcgg ctcctgccga gcgccactat cctcgcgggc
     2461 aagcgggtgg cgcgcgccta gccaccgctc tagggcttgc ccagtcccat catggcgagg
     2521 gcggcgcgcc ccgcccagcc atagggattg gccattgtct cctgcaattc ataatgattg
     2581 tggttgggag cgacgatcag cgtgaccggc tttcccgcgg ctttcacggc ggccgcgaaa
     2641 tcgcggcttt ggcgctggaa ttccggcgtc tcattggtgc catagagcag cgtgatcggc
     2701 gtgtgcagcc tgtcgatgtg gcggatggtg ctcatggaat cttccatggc gtcgtcgaac
     2761 ttcacatatt tgctgcgcgc cgacagccgc acgggcttca tctcatacat gcccgagagc
     2821 agcacaccgc ccttgacgat gtcggcgggc aggccaaaat ccttctgcca atcggtgatg
     2881 agcgccacgc ccgacagatg cgcgcccgag gattggccgc acagatagat atggttggga
     2941 tcgcccccga aactcgccgc gttcttataa acccag
//
LOCUS       DASYEG010000348         1574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_863107, whole genome shotgun sequence.
ACCESSION   DASYEG010000348 DASYEG010000000
VERSION     DASYEG010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863107"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VGM72_14540"
     CDS             complement(<1..527)
                     /locus_tag="VGM72_14540"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY4266532.1"
                     /translation="MNNPALTFRAPLEQANSGWRRYAGISLAIALNGVLVWALASGLA
                     IKLTKYVPPVLETKVIDAPQAQPKQVTPPPTPQIEKIVPTQQTPEPVIQIVPDPNAQS
                     IQVQPQQQQPMQATDSSASGMTSTHTSPPYPPVARRLGQQGVVILAITVAADGSVSAA
                     QVATSSGVPELDQAAL"
     gene            complement(687..1052)
                     /locus_tag="VGM72_14545"
     CDS             complement(687..1052)
                     /locus_tag="VGM72_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4266533.1"
                     /translation="MAMAHILLAEDDESLRRFLAAALVKAGHTVTDFGDGDEAYACLK
                     GVRFDLLLTDIVMPGMDGIELAKRAAEMDSALKIMFITGFAAVALHPSSQAPKQAKVL
                     SKPFHLREIVQEVERMIAA"
     gene            1197..>1574
                     /locus_tag="VGM72_14550"
     CDS             1197..>1574
                     /locus_tag="VGM72_14550"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEY4266534.1"
                     /translation="MRRDGAQQTDGAGDSALESLHARSFDLFLPERQRVPFVFASPHS
                     GRVYPRSFASASRLSPLGLRRSEDAFVDELFANAIELGAPMIAARFPRAYLDANRAPS
                     ELDPAMFEGVLTMPVDPPGTRVNA"
BASE COUNT          286 a          490 c          482 g          316 t
ORIGIN      
        1 agcgccgcct ggtcgagttc gggcacgccg ctcgatgtcg cgacctgcgc cgcggacacc
       61 gaaccatccg ccgccacggt gatcgccagg atcaccacac cctgctggcc cagcctgcgc
      121 gccacgggcg gataaggcgg cgaggtatgc gtgctggtca tgcccgaagc cgaactgtcc
      181 gtggcttgca tcggctgctg ttgttgcggc tggacttgga tgctttgcgc attcggatcc
      241 ggcacgatct ggataaccgg ctccggcgtc tgctgcgtcg gaacgatctt ttctatttgc
      301 ggtgtcggcg gcggcgtaac ctgttttggc tgcgcttgcg gcgcgtcgat cactttggtt
      361 tcgagcaccg gtggaacgta tttggtgagc ttgatcgcaa gcccgctcgc cagcgcccac
      421 accaatacgc cgttcaacgc tatggcgagg gaaattccgg cgtagcggcg ccaaccgcta
      481 ttggcctgtt cgagcggtgc gcgaaacgtc aatgcggggt tgttcataat attgctcccg
      541 ttcaaaaaaa tgcgcggatc gcagacacgc cggaagcttt ctcccggcgc acccgcgaga
      601 ccctgcaaat acaacgcagg aatgtggcta ttgccgggtt tttgaacggc gctttacggg
      661 aggttgtgtg gaactttgtg ttccagtcag gccgcgatca tgcgctccac ttcctgcacg
      721 atctcgcgca ggtggaaggg cttggacaga accttcgcct gtttgggcgc ctgcgatgac
      781 ggatgcaggg ccacggcggc aaagccggtg atgaacatga tcttgagcgc gctatccatt
      841 tccgcagcgc gcttggccaa ttcgatgccg tccatgccgg gcatgacgat atcggtcagc
      901 agaagatcga agcgcacacc cttgaggcag gcataggctt catccccatc gccgaaatcg
      961 gtgacggtgt ggccggcttt caccagcgca gcggcgagaa atcgccgaag cgattcatcg
     1021 tcttccgcca gcagaatatg cgccatggcc atggatcccc tcccccggaa taaaccctat
     1081 taccggcggc cggtaaatgt cgttaacgcg cgactttaca gccgccccaa agggttgatt
     1141 ggcaggccgc aggaatggtc cactcgcttc gcaaacattg ggaatcggca gcaagcatga
     1201 ggcgagacgg cgcacagcag acagacggag ccggagattc cgcgctcgaa agcctgcatg
     1261 cgcgcagctt cgatcttttc ctgcccgagc ggcagcgcgt gcccttcgtc ttcgcgtcac
     1321 cccattccgg gcgcgtctat ccgcgcagct tcgcatcggc gagccggctg agcccgcttg
     1381 gcttgcgccg ctccgaagat gctttcgtcg atgaattgtt tgccaatgcg attgaactcg
     1441 gcgcgccgat gatcgccgcc cgttttccgc gcgcatatct ggacgccaat cgcgcgccaa
     1501 gcgagctgga ccccgcgatg ttcgaaggcg ttctcaccat gccggtcgat ccgccgggaa
     1561 cgcgcgtgaa tgca
//
LOCUS       DASYEG010000349         2656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_1013782, whole genome shotgun sequence.
ACCESSION   DASYEG010000349 DASYEG010000000
VERSION     DASYEG010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013782"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="VGM72_14555"
     CDS             complement(<1..1314)
                     /locus_tag="VGM72_14555"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEY4266535.1"
                     /translation="MQHSLFATAEDRAAPGTGGHYANYALGAAYDEMFTPDRRVRPQY
                     AELHQRVGALPSDELRRSQQACEQSFLHQGITFTVYGENQSTERIIPTDLLPRIITAA
                     EWARIEAGLKQRIQALNLFLHDIYAEGRVLKDGLVPRALIYGSKHYRREMRGIPVPHG
                     AYVNVCGTDLVRREDGTFVVLEDNLRVPSGVSYMLANRDVVRRAFPALFRTAGVRPIE
                     HYPQDLLATLKSLAPSQDDVSIVVLSPGVFNSAYFEHALLARQMGVELVEGRDLVVND
                     NSVHARTTSGLKRVDVIYRRIDDDFIDPLAFRSDSTLGIPGVFNAYRAGNVVIANAPG
                     TGVADDKAVYAYVPRLIRYYLSEEPILDIVETFLCREPKSLSHVLTNLDTLVVKAVGE
                     SGGYGMLIGPHASKAEREDFARRLKAEPDNYIAQPTIQLSTAPCFI"
     gene            complement(1442..2356)
                     /gene="prpB"
                     /locus_tag="VGM72_14560"
     CDS             complement(1442..2356)
                     /gene="prpB"
                     /locus_tag="VGM72_14560"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: protein motif:HMM:TIGR02317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEY4266536.1"
                     /translation="MPFLVGADLPDRPAGERFRALLSRSQILQIPGAHTGMAALLAKR
                     AGFEALYVGGSAMTGTMGLPDLGIITVEEVSFFIRQVARASSLPVLVDGDTGHGEALN
                     VMHMVRSFEDAGAAAVHIEDQLLPKKCGHLNDKKLADAREAAAKIAAAIKARRHLFII
                     ARTDAADSEGMDGAVARAKLYLEAGADAIFPEALTNAQMFREFAARVGGPLLANMTEF
                     GRTPVFTASEFEAMGYRMVIWPASAMRVAAKAQDELYAAIKRDGGTHGMLARMQTRQE
                     LYDTIGYSDYEMLDASIVASVTPRSPAR"
     gene            complement(2371..>2656)
                     /gene="prpD"
                     /locus_tag="VGM72_14565"
                     /gene_synonym="mmgE"
     CDS             complement(2371..>2656)
                     /gene="prpD"
                     /locus_tag="VGM72_14565"
                     /gene_synonym="mmgE"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="functions in propionate metabolism; involved in
                     isomerization of (2S,3S)-methylcitrate to
                     (2R,3S)-methylisocitrate; also encodes minor aconitase or
                     dehydratase activity; aconitase C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HEY4266537.1"
                     /translation="QFTRDYLDPEKRSIANGIIVRFADGTILPEAAIEYPIGHRRRRT
                     EGIPLLVEKFRTNLARRFTSERQQIILDASLDGARLDAMNVNDYVDLYII"
BASE COUNT          512 a          812 c          851 g          481 t
ORIGIN      
        1 gatgaaacag ggcgcggtgg acaattggat cgtcggctgc gcgatgtaat tgtccggctc
       61 ggccttcagc ctccgcgcga aatcctcacg ttctgccttc gaggcatgcg ggccgatcaa
      121 catgccgtag cccccggatt ctcccaccgc cttcaccacc aatgtgtcga ggttggtgag
      181 cacatgtgag agcgatttgg gctcgcggca gagaaaggtc tcgacaatgt cgaggatcgg
      241 ctcctcggaa agataatagc ggatgagccg cggcacatag gcatagaccg ccttgtcgtc
      301 ggcgaccccg gtgcccggtg cattggcgat caccacattg cccgcgcgat aggcgttgaa
      361 cacgccagga atgccgagcg tcgaatccga gcggaaggcc aacgggtcga tgaaatcgtc
      421 atcgatgcgc cgataaatca cgtcgacacg tttcaagccc gacgtggtgc gcgcatgcac
      481 cgaattgtcg ttgacgacaa gatcgcgccc ctcgacaagc tccacgccca tttggcgcgc
      541 cagcaacgca tgttcgaaat aggccgaatt gaagacgccc ggcgacagca ccacgatcga
      601 gacatcgtcc tgcgagggcg cgagcgattt gagcgtcgcg agcaaatcct gcggataatg
      661 ttcgatgggg cgaacgccgg cggtgcggaa cagcgccgga aaggcacgcc gcaccacatc
      721 gcgattggcc agcatgtaag agacgcccga ggggacgcgc agattgtctt cgagcaccac
      781 gaaggtgccg tcttcgcgcc gcacgagatc ggtgccgcag acattgacat aggcgccgtg
      841 cggaaccgga atgccgcgca tctcgcgccg gtaatgcttg gagccgtaga tcagcgcacg
      901 gggcaccagc ccatccttga gcacacgccc ttcggcatag atgtcgtgca ggaaaagatt
      961 cagcgcctgg atgcgctgtt tcaaaccggc ttctatacgt gcccattccg ccgctgtgat
     1021 gatgcgcggc aggagatcgg tcggaatgat ccgctcggtg gattggtttt cgccgtagac
     1081 ggtgaaggtg atgccttgat gtaggaagga ttgttcgcaa gcctgctggc ttcggcgcaa
     1141 ttcgtcggaa ggcagcgcgc cgacccgctg atgcagctcc gcatattgag gccgcacccg
     1201 ccggtccggc gtgaacatct cgtcataggc agcccccaac gcataattcg cgtaatgccc
     1261 gccggtgcct ggcgcggccc gatcttcagc ggtcgcgaaa agggaatgct gcatgtgcga
     1321 aggggacagg aaatgagccc gccccgtcaa ggggctcagg cccgcaacca tgtcgcagtc
     1381 acgcaaaaca gggccgcgat ggcttagatc ggcgttgcca cgacaagccg ggccccgatg
     1441 atcaccgcgc cggacttcgc ggcgttacgc tcgcgacgat gctggcatcg agcatctcgt
     1501 aatcggagta gccgatggtg tcgtatagtt cctggcgcgt ctgcatccgt gccagcatgc
     1561 cgtgcgtgcc gccatcccgc ttgatcgctg cgtaaagctc gtcttgggct ttggccgcga
     1621 cccgcatcgc gctcgccggc cagatgacca tgcgatagcc catggcttcg aactcgctcg
     1681 cggtgaacac gggcgtgcgg ccgaattcgg tcatgttggc cagcagcggt ccccccacgc
     1741 gggcagcgaa ttcgcggaac atctgcgcat tcgtcagcgc ttcgggaaaa atcgcatcgg
     1801 cgcccgcttc gagataaagc ttcgcgcggg ccaccgcgcc gtccatgcct tcgctatccg
     1861 ccgcatccgt gcgcgcgatg atgaagaggt ggcgccgtgc cttgatggcg gcggcgatct
     1921 tggcggcggc ctcacgcgca tcggcgagtt ttttgtcgtt cagatggccg catttcttgg
     1981 gcagcaattg gtcttcgatg tggacggccg cagcaccggc gtcctcgaaa ctgcgcacca
     2041 tgtgcatgac attgagggcc tcgccgtggc cggtatcgcc atccaccagc accggcaggc
     2101 tcgatgcccg cgccacctgg cggatgaaga acgacacctc ttccaccgtg atgatgccga
     2161 gatcgggaag acccatggtg ccggtcatcg cgctgccgcc gacataaagc gcctcgaagc
     2221 cggcgcgttt ggcgagcagt gcggccatgc ccgtatgcgc accggggatt tgcaggatct
     2281 gagaccggga gaggagggcg cggaagcgct cgcccgcggg cctatcgggc agatcggcgc
     2341 cgaccaagaa gggcatgcat gcctcccatt tcagatgatg tagagatcga cataatcgtt
     2401 gacgttcatg gcgtcgagtc gcgccccatc gagcgacgcg tcgaggatga tctgctgccg
     2461 ctcggacgtg aagcggcgcg cgagattggt gcggaatttc tccaccagaa gcggaatgcc
     2521 ttcggtgcgc cgccgccggt gcccgatggg atattcgatc gcggcttcgg gcagaatcgt
     2581 tccatcggca aagcgcacaa tgattccgtt ggcgatcgag cgtttctccg gatcgagata
     2641 gtcgcgggtg aattgc
//
LOCUS       DASYEG010000350         2181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4491504, whole genome shotgun sequence.
ACCESSION   DASYEG010000350 DASYEG010000000
VERSION     DASYEG010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4491504"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..942
                     /locus_tag="VGM72_14570"
     CDS             166..942
                     /locus_tag="VGM72_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266538.1"
                     /translation="MFDIILAREAKHASFPAGHFPVIAVFAFWIAAFASVPGAQAAAG
                     PAAVPDISGVWWATTYSPKIPLVGGGDLPYTDAGKAAYEKNVAALKSGERIDAARRFC
                     VPDGVPRVLATPYPFEIIQTPGQTTILYELNHAIRVVAMDKPLPKDEELIPYPYYDGH
                     SAGHWDGDTLVVETAGFNDKTFLDATGAPHSDAMKTVERIRKTGSQLEDVVTVHDPDM
                     FTRDWSARFVYALRNDIRLRDYVCGEKHRDISSVRGIQGR"
     gene            974..2116
                     /locus_tag="VGM72_14575"
     CDS             974..2116
                     /locus_tag="VGM72_14575"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY4266539.1"
                     /translation="MRALDGIIVLEFANYVSGPYAGMMLADLGAEVIKIESPDGGDPF
                     RGWGAADYSATFGSVNRNKKSVALDLKTADGLAAALALARTADVLIENFRAGTMDRLG
                     LGYAKLSDANPRLIYTSITGFGGSGPYAERAGYDTVGQAMSGLLSVLTDLTNPQPMGI
                     SLSDHLTGMMAAYGILGALMARGQTGRGQRVETSLLAASIAFLGENAARFFETGKTPS
                     RETRARSAQVYAFVDAKGEGFVVHLSSPTKFWRGLTEVAGHPEWADDARFTKKRDRQE
                     NYRVLHRLLGEIFVTRERDHWLALLQARDVPSAAINTLDQVFADPQVKHLGLIREVPH
                     PKLGHVSLVGGGVNLSETPPEIRSAAPDLGQHNEEVLARIAMAKTT"
BASE COUNT          414 a          650 c          690 g          427 t
ORIGIN      
        1 ctgtgatcca tcccctgaag gacggcaccc acggcggcca gttcatggcg gtcacgcttc
       61 cggatggaac gctgaaaggc aatccgaatg gagcagcggg cgccaacgcg ggcgcggcag
      121 ccaattagcc cgaagattgc acccatccgg tctcgggtga atgtaatgtt cgatatcata
      181 ctggccaggg aggcgaagca cgcttcgttt cccgccggcc attttcccgt aattgcggtc
      241 tttgcatttt ggatcgcagc ttttgcgtct gttcccggtg cgcaagctgc cgccggacct
      301 gccgccgtgc ccgatatttc cggagtctgg tgggcgacca cttacagtcc caaaatcccg
      361 ctcgtgggcg gcggcgatct tccgtatacg gacgctggaa aagcggccta tgagaagaac
      421 gtcgctgcgt tgaaaagcgg agagcggatc gatgccgccc gccgcttctg cgtgcctgac
      481 ggcgtgccga gggttctcgc gacgccctat ccgttcgaga tcattcagac gccggggcag
      541 accaccatcc tctacgaact caaccatgcg atccgcgtgg tggcgatgga taagccgctg
      601 cccaaggacg aggaactgat cccctatccc tattatgacg gccattcggc cggccattgg
      661 gatggcgata cgctggtggt cgaaacggcg ggcttcaacg acaagacgtt ccttgacgcc
      721 accggtgccc cgcacagcga tgcgatgaag acggtggaac gcatccgcaa gacgggtagc
      781 cagctcgaag atgtggtcac ggtgcacgac cctgacatgt tcacgcggga ttggagcgca
      841 cgcttcgtct atgctctgcg caacgacatc cggctgcgag attatgtttg cggcgagaag
      901 caccgcgata tctcatcggt gcgcggaatc cagggtcgtt gacgattccg ttgcaataag
      961 ctttaggcca tccatgcggg cgcttgacgg aatcattgtc ctggaattcg ccaattacgt
     1021 cagcggcccc tatgccggga tgatgctggc cgatctcggt gccgaagtga tcaagattga
     1081 atcgcccgat ggcggcgatc cgtttcgcgg ctggggtgcg gccgattaca gcgccacgtt
     1141 cggttcggtg aatcgcaaca agaaaagcgt ggcgctcgat ctgaagacgg ccgacgggct
     1201 tgcggcggcg ttggcgctcg cacgtaccgc cgatgtgttg atcgagaatt ttcgtgccgg
     1261 caccatggat cggctgggcc tcggctatgc gaagctctcg gacgccaatc cacggctgat
     1321 ctacacctcg atcaccggct tcggaggctc cggtccttac gcggagcgtg ccggctatga
     1381 tacggtgggc caggcgatga gcgggctttt gagcgtgctc accgatctca caaatccgca
     1441 gccgatgggg atttcactct ccgaccatct gaccgggatg atggcggcct atggcatatt
     1501 gggcgcactg atggcccgcg ggcaaaccgg acggggccag cgggtggaga catcgctgct
     1561 ggcggccagc atcgcgttcc ttggcgagaa tgcggcgcga ttcttcgaga ccggcaagac
     1621 gccatcgcgc gagacgcggg cgcgttccgc acaggtttat gcctttgtcg atgcgaaggg
     1681 cgaaggtttt gtcgtgcatc tgtcctcacc cacaaaattc tggcgcggcc tgaccgaggt
     1741 cgcgggccat cccgaatggg ccgacgatgc gcgctttacg aagaaacgcg atcgccagga
     1801 aaattaccgc gtgctgcacc gattgctggg ggaaattttc gtgacgagag agcgcgatca
     1861 ttggctcgcg ctcctgcagg cgcgcgatgt tccgagtgcc gccatcaaca cgctggatca
     1921 ggtgttcgcc gatccgcaag tgaagcatct cggtctcatt cgcgaagtcc ctcatcccaa
     1981 gctcgggcat gtgtcgctgg tcggaggcgg ggtgaatttg agtgaaacgc cgcctgagat
     2041 tcgcagcgcc gcccccgatc tcggtcagca taatgaagaa gtgctcgccc gcattgccat
     2101 ggcgaagacc acatgatcgc gccctcgcaa cgcttgcgca atgcctgcgc cattgtcggg
     2161 gccggcaaca gccgcctggg a
//
LOCUS       DASYEG010000351         4067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_2607540, whole genome shotgun sequence.
ACCESSION   DASYEG010000351 DASYEG010000000
VERSION     DASYEG010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2607540"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VGM72_14580"
     CDS             complement(<1..653)
                     /locus_tag="VGM72_14580"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY4266540.1"
                     /translation="MLEKTFRSGEVEARLYAQWEDSGAFRAGSKPGVPGFSIVIPPPN
                     VTGSLHIGHALNNTLQDALARFERMRGKNVLWQPGTDHAGIATQLVVERELAKRQTSR
                     TKLGREKFLEEVWRWKEESGGTIVRQLRRLGASCDWSRERFTMDEGLSRAVLKVFVAL
                     HRQGLIYKDKRLVNWDPRLQTAVSDLEVESIEVKGKLWHIKYPLAGGATFQWPVFDE"
     gene            complement(767..1381)
                     /locus_tag="VGM72_14585"
     CDS             complement(767..1381)
                     /locus_tag="VGM72_14585"
                     /inference="COORDINATES: protein motif:HMM:NF022155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HEY4266541.1"
                     /translation="MSSSANPQHEPTMEEILASIRKIISEDQTEEKPADGASPVQAVA
                     SAPADQAAGGEADVLELTEELSEEEPEPPVPEPAAQIEDDIAFESVEAEPKVEAPMDD
                     LISDSARSAMDRAFAHLDRGGRELAAPAAAATGGTLDALFLRAIQDAFTPTLLEWVEG
                     HHAEILEQMKPLIRDWMDENLPSLIEAAVTKEISRAANARGRSR"
     gene            1535..2611
                     /locus_tag="VGM72_14590"
     CDS             1535..2611
                     /locus_tag="VGM72_14590"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEY4266542.1"
                     /translation="MMADFVTILSRRRAEVRHGLRIAAAGMLSYVTAHLLGLPQGYWA
                     VFTAVLVVQGSVGGSWKAAVDRLAGTLFGAIYGAAIATLVPHNDVLMLGIALAIALTP
                     LAWLAAFYPSYRVAPITAVVLLLGGTASVEGPFIAAVLRTVEVTLGGFIGIAVSLFVL
                     PARAHALMGDAANRVLQRLAALLGDLMEGLATPVDAKAIIARHDAVRAALTALENVSD
                     EAAREKRNYLTDDSDPEPIARTLRRVRHDLVLIGRIAAEPLPEDPGSPLKRALAAYST
                     VATEFLRAIGTAFAERASPPPLDGFIAAERRVLEAMHAIKGDEKLVALAFGFEQFQRN
                     LHDLVRRAQEFARATVKSADDRES"
     gene            complement(2608..3264)
                     /locus_tag="VGM72_14595"
     CDS             complement(2608..3264)
                     /locus_tag="VGM72_14595"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEY4266543.1"
                     /translation="MMLDTADQRANMVAAQLRPNDVTDARIRDAMLSVPRERFVPEGF
                     APVAYMEGTIPIGPGRVLLDPRSFAKLLQLAGVGPNDHVLDVACGTGYSTAILALMGR
                     DVVGLEDNAELAARAAANLGDLGAANARVIGGPLVSGCPAEAPFDVIFLNGAIERQPV
                     DLLGQLADGGRLVAIVRNGAAGHAFVYLKHQGAVGERSAFDACVPVLPGFETPPRFVF
                     "
     gene            3849..>4067
                     /locus_tag="VGM72_14600"
     CDS             3849..>4067
                     /locus_tag="VGM72_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4266544.1"
                     /translation="MKLAPPLSLSVLLTAASLPAHCAEVGPLAPGGAAGLKAAQAEGA
                     TPTMWIVGAGALVGVVAAIALSNHGNAIA"
BASE COUNT          704 a         1287 c         1277 g          799 t
ORIGIN      
        1 tcttcatcga agaccggcca ttggaaggtc gcgccgccgg cgagcggata tttgatatgc
       61 cagagcttgc ccttgacctc gatgctctcg acttcgagat cggagacggc ggtctgcagc
      121 cgcgggtccc aattgacgag gcgcttgtcc ttataaatca ggccctgccg atgcagcgcg
      181 acgaacactt tcagcacggc gcgggagagc ccctcgtcca tggtgaagcg ctcgcggctc
      241 cagtcgcagg aggcccccag ccggcgcaat tgccgcacga tcgtgccgcc ggattcctct
      301 ttccagcgcc agacctcttc gaggaatttt tctcggccca gtttggtgcg cgaggtttgg
      361 cgcttggcaa gctcccgctc gaccaccaac tgcgtggcga tgcccgcatg atcggtgccc
      421 ggctgccaca gcacgttctt gccgcgcatg cgctcgaaac gggcgagggc gtcctgcaat
      481 gtattgttga gggcatggcc gatatgaagg ctgccggtca cattgggcgg cggaatcacg
      541 atggaaaaac cgggaacccc cggtttggac ccggcgcgaa aggcgccgga atcctcccat
      601 tgcgcataaa gacgggcttc cacctcgcca gatcgaaagg ttttttcgag catgatgatc
      661 ctgaaaattc agcttttgct gaaaggacgg cgcacagggc cctatcgtca agagaaaaga
      721 gcgcgtcttc ctttcggaag gctgctgcgc ggcccggttg tggattctac cggctgcggc
      781 cgcgggcgtt cgcggcgcgc gagatttcct tggtgaccgc ggcctcgatc aggctgggca
      841 gattttcgtc catccaatcg cgaatcaggg gcttcatctg ctccaggatc tcggcgtgat
      901 ggccttcgac ccattccagc agcgtcggcg taaaggcatc ctggatcgcc cgcaaaaaca
      961 gcgcatccag cgtgcccccg gtcgcggctg cggccggagc ggcgagttcc cggccgccgc
     1021 ggtccagatg agcgaatgcg cggtccatgg cgctgcgcgc ggaatcggag atcagatcgt
     1081 ccatcggggc ttccactttc ggttcggctt ccacgctttc gaacgcgata tcgtcttcga
     1141 tttgcgcagc aggttcggga accggcggtt cgggttcttc ttcgcttagc tcctcggtca
     1201 gctccagaac gtcggcttcg ccaccggcgg cttgatcggc gggcgcgctc gcgacagctt
     1261 ggaccgggct tgccccgtcc gcgggtttct cttccgtctg atcttccgaa atgatttttc
     1321 tgatggaggc gagaatttcc tccattgttg gttcatgctg cggattggca gaactgctca
     1381 ttggtactct ctcgactggg cggcggccgt tgcgcggttt caggccgttt ggaccctaag
     1441 gcagaagcta taggaaagcc atattagtta atgttgcatt tacgatcatg atgcgtttgc
     1501 gttcgcaccg aacggcacac agaattttcg gctgatgatg gcggatttcg tcaccatttt
     1561 gtcgcgtcgc cgtgccgagg tcaggcacgg cctgcgcatc gcggccgccg gaatgctgag
     1621 ctatgtgacc gcccatctgc tcggcctgcc gcagggctat tgggccgtgt tcaccgcggt
     1681 gctggttgtg cagggaagcg tgggcggctc gtggaaggct gccgtggacc gcctcgccgg
     1741 caccctgttc ggcgcgattt acggagcggc catcgccact ctggtgccgc acaacgacgt
     1801 gctgatgctg gggatcgcgc tcgcgatcgc gctgacgccc cttgcatggc tcgcggcgtt
     1861 ctacccgagc tatcgcgtcg ctcccatcac ggcggtggtt cttctgctcg gcggcacggc
     1921 cagcgtcgaa ggtccgttca tcgccgccgt gctgcgcacg gtggaggtca ccctgggcgg
     1981 ctttatcgga atcgccgtct cgctgttcgt actcccggcg cgggctcacg cgctgatggg
     2041 cgatgcggcc aatcgcgtgc tgcagcgttt ggccgcgttg ctcggcgatc tgatggaagg
     2101 cctcgccacg ccggtggatg caaaggccat catcgcgcgc catgatgcgg tgcgcgcagc
     2161 actcaccgcg ctcgagaacg tttccgacga agccgcgcgc gaaaaacgca actacctcac
     2221 cgacgattcc gaccccgaac ccatcgcgag gacccttcgg cgggtgcgcc acgacctggt
     2281 gctgatcggg cgcatcgccg ccgaaccttt gcccgaagac ccgggctcgc cgctcaagcg
     2341 cgccttggcg gcgtactcca cggttgcgac cgagttcctg cgtgcgatcg gaacggcctt
     2401 tgccgagcgt gcatctccgc caccgctcga cgggttcatc gccgccgagc gccgcgtatt
     2461 ggaagcaatg cacgcgatca aaggcgatga aaagctcgta gcgctggcct tcgggttcga
     2521 gcaattccag cgaaatcttc acgatctggt tcgccgggcg caggaattcg cacgcgccac
     2581 cgtgaaatcc gcagacgacc gcgaatctta gaacacgaag cggggcggcg tctcgaaacc
     2641 gggcagaacc ggcacacacg cgtcgaacgc gctgcgctcg ccgacggcgc cttggtgctt
     2701 gagatagaca aaggcgtggc cggcggcgcc gttgcgcacg atcgccacga gccgtccgcc
     2761 atcggcgagc tggccgagca gatccaccgg ctggcgttcg atggccccgt tgaggaaaat
     2821 cacatcgaat ggagcctcag ccggacagcc ggacaccaag ggcccgccga tcacccgcgc
     2881 attggccgca cccagatcac ccagattggc ggccgcgcgc gcggcaagtt ccgcattgtc
     2941 ctccagaccc acgacatcgc gccccatgag cgcaaggatc gcggtcgaat agcccgtgcc
     3001 gcaggcgaca tcgagcacat gatcgttcgg ccctacgccg gccaattgca gcagcttggc
     3061 aaaggagcgc ggatcgagaa gcaccctccc cggcccaatg ggaatggtgc cttccatata
     3121 ggccacgggt gcaaacccct cagggacaaa gcgctcgcgt gggacgctca gcatggcatc
     3181 gcggatgcgc gcatcggtca cgtcgttggg acggagctgg gcagcgacca tgttggcgcg
     3241 ctggtcggca gtgtcgagca tcatttcatg aatccggcaa aaggaagcgg gtgccgccgc
     3301 ttatagtggc atggggtgcg gctgacaatt catggcacga gcaaggttgt gggccgcggc
     3361 cggcgatctg atataggcgg gctcacgggt gccggcttcc cctgcacctg caggattcgg
     3421 ccatgtgggg gagtggtgac gcaagggtct gcggaatcgt gcagaggcgt cacggccatt
     3481 ccatctcgtc ttttttccca agcttttgcg gcatttcggg gccttacccg cttgtccccg
     3541 ctaatccggg ttcgtgctaa cgcgcgggcc atcgcgctgt agctttagtg atcgccctag
     3601 cgattccact acgcgtcctg aatcacccgg gagtgccggc ggataccctc tatcgcggcg
     3661 ccttcgtctt cttccgcaac gacatagaga agccgcatgg gtgtttcccc ccttcgcgaa
     3721 gagggatcgt cacgcggaca agatagccgc caatcatagc cggtccacgt tatccaacat
     3781 tcaattccgt cacgcgttaa ctatagaaag catgagccag gcttttgcca agctgctacg
     3841 tgttttgcat gaagctcgca cctccattat ccttgagcgt cttattgacg gctgcatcat
     3901 tgcccgcgca ctgcgctgag gtcggtccat tggctcccgg cggcgccgcg ggattgaaag
     3961 ccgcccaggc tgaaggcgcc actccaacaa tgtggattgt tggcgcgggt gcgctagtcg
     4021 gtgtcgtcgc tgcaatcgca ctcagcaacc acggcaacgc gattgcg
//
LOCUS       DASYEG010000352         4200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Micropepsaceae bacterium isolate SMAG_U4979
            k141_4058393, whole genome shotgun sequence.
ACCESSION   DASYEG010000352 DASYEG010000000
VERSION     DASYEG010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958253
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Micropepsaceae bacterium (soil metagenome)
  ORGANISM  Micropepsaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Micropepsales;
            Micropepsaceae.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 13:00:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,924
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Micropepsaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4058393"
                     /isolate="SMAG_U4979"
                     /isolation_source="soil"
                     /db_xref="taxon:2910169"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VGM72_14605"
     CDS             complement(<1..966)
                     /locus_tag="VGM72_14605"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY4266545.1"
                     /translation="MARTKKALPNSSNETQHWRAIDAAHHIHPFTQTEALVREGVRVI
                     TRAKGIYLWDSDGRKLIDGMSGLWCVQLGYGNKELARAGCDALQTLPYYNHFFKTTNP
                     WTAELAAKLTALLPEGHRHVLFANSGSEATDTALKLIRYYWNLKGRPEKKIHLSRTLS
                     YHGVTMAAASLSGLTPLHPQWDLPLPGFAKVPAPYWYGAKATGYGDIGADEFGIAIAR
                     DMEKIIREIGPERIASFSAEPVQGAGGLIVPPKTYWPEVSRICAENDILLHSDEVITG
                     FGRTGEWFGSFTYDLKPDIVTMAKGLSSGYQPISAISLGARMGEAI"
     gene            complement(1092..2681)
                     /locus_tag="VGM72_14610"
     CDS             complement(1092..2681)
                     /locus_tag="VGM72_14610"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY4266546.1"
                     /translation="MIEPIRLLGFAFASADFLFEIDREGTILFATGAVSLFATQTELQ
                     GISAAELFQPSEHQRFAAIVRGLAPGGRIGPLPTILASGEKASLSMCFLPQSDRISCA
                     LVKPGKRGGLTAGKDKQTGLADNDAFLAAAAESAGGRGAVAMVNVPNLPDVCAKLSPE
                     DASALLAGIGANVRTMGAVVAARLSETRFGVVTDDPRGARNLAEKIRGAARERGLESL
                     QVEEVLLSLKGRNLTPEQNILALRYAVTRFAEGKIKSVTDADLAGMFDRLMDETLARA
                     HDFNATVAQSAFDLVFEPIVDLKTGTPSHYEVLTRFQPGQSPGEIIKFAEELGLTDAF
                     DMAVALKAFGILERDSAIAASIAINISGRSFANSATFGVLAGLLAKKRAFAKRVLIEV
                     TESAEMPDLVAADKAIQSLRLMGYRVGIDDFGSGAASLQYLHSFTIDFVKIDGAVIQR
                     MGKSPREDALLKSMLSTCAELHIETVAEWIDSPEKLQRCRDIGFQHGQGRHFGGSLTE
                     LPKVAVAVPRARREGVKVSWG"
     gene            complement(2777..3577)
                     /locus_tag="VGM72_14615"
     CDS             complement(2777..3577)
                     /locus_tag="VGM72_14615"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEY4266547.1"
                     /translation="MRTKLPIVGIVCDRKKVGDHAFHMVGEKYIEAVRDGSGALPFLI
                     PALRPPLDLGDILAGVDGLLFTGSTSNVAPHRYGGEPARRHTPLDEARDATAIPLMRA
                     AEAAGVPMLCICRGFQELNVAFGGTLHQHLHEVEGFADHRAGDRKESLDEQYGPIHDV
                     RVVPGGLLTELLPELAPGDSFQVNSLHGQGIARLAHDLRVEAEAPDGAIEAVSMDRAP
                     AFLFGVQWHPEWRHGENPVSRAIFAAFGDALRLRAHERGRALLEVVAR"
     gene            complement(3628..>4200)
                     /locus_tag="VGM72_14620"
     CDS             complement(3628..>4200)
                     /locus_tag="VGM72_14620"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HEY4266548.1"
                     /translation="PIAPVFSSAEKADLKRVSAYLNTIQSVQGRFTQVDADGKTERGA
                     FYLKKPGRVRFEYEKPNPNLIIADGSTVAVENSALKTTDRYPLTNSPLRLLLSDDIDL
                     TNDSRVSALKREPGAISVTAREMSAVASGSITLTFADSGGRLELRQWEVVDAQGARTM
                     VVLNDMRQAADIPPRLFVIEDLSPFKKSGR"
BASE COUNT          828 a         1284 c         1298 g          790 t
ORIGIN      
        1 gatcgcttcg cccatgcgcg cgcccagcga gatcgcggag atcggctggt agcctgagga
       61 aagacctttc gccatcgtga caatgtcggg cttgagatca taggtgaaac tgccgaacca
      121 ttcgcccgtg cggccaaagc cggtgatgac ttcatcggaa tgcagaagaa tatcgttttc
      181 cgcgcagata cgggacactt ccggccaata ggttttgggc ggcacgatca agcctccggc
      241 cccctgcacg ggttcggccg aaaaagaagc gatgcgttcg ggtccgatct cgcgaatgat
      301 tttttccata tcgcgcgcga tcgcgattcc gaattcatcg gcgccgatat cgccatagcc
      361 cgtggccttt gcgccgtacc aatagggcgc agggactttc gcgaagcccg gaaggggcaa
      421 atcccattgc gggtgcagag gcgtcaggcc ggaaagcgag gcggcagcca tcgtcacgcc
      481 gtgataggac agcgtgcgtg aaagatggat cttcttctcc ggacggccct tcaaattcca
      541 gtaatagcgg atgagcttga gtgcggtatc ggtcgcttcc gaaccggaat tggcgaacag
      601 cacatggcga tggccttccg ggagcagcgc cgtgagtttc gcggccagtt ccgccgtcca
      661 aggattcgtc gtcttgaaaa aatgattgta atagggaagc gtctggagcg cgtcgcagcc
      721 ggcgcgggcg agttccttgt tgccgtagcc caattgcacg caccacagtc ccgacatgcc
      781 gtcgatcaat ttcctgccgt cggaatccca gagatagatt cccttcgcgc gggtgatgac
      841 ccgcacacct tcgcgtacca gcgcttcggt ctgggtgaag ggatgaatat ggtgggcggc
      901 atctatcgcc cgccaatgct gggtctcatt gctggagttc ggcagggcct tcttcgtgcg
      961 ggccatttgt catgtccttc cggtcgcgca gccctcgaaa ggcggcgcca ggattatggg
     1021 cgaccggatt acgtcaatag gggaaattgt atccgaaacg aagcgagttt ctgaagcgaa
     1081 gaactttggc ctcagcccca actgaccttg acgccctcgc gccgggcgcg cggaaccgca
     1141 accgcaacct tgggaagctc tgtaagagaa ccgccgaaat ggcggccctg accgtgctgg
     1201 aatccgatat cgcggcaacg ctgaagcttt tccgggctgt cgatccactc agcgaccgtc
     1261 tcgatatgga gttcggcaca ggtggacagc atgcttttga gaagggcgtc ctcgcggggg
     1321 gatttgccca ttctctggat cacggcgccg tcgatcttca cgaaatcgat cgtgaaactg
     1381 tgcagatatt gcagcgatgc ggctcccgaa ccgaaatcgt cgataccgac ccgataaccc
     1441 atcagccgca atgactggat cgccttgtcc gcggccacaa gatcgggcat ctcggcgctt
     1501 tcggtcacct cgatcagcac gcgtttcgcg aaggcgcgct ttttcgcaag caggcccgca
     1561 aggacaccaa acgttgccga attggcgaag ctgcgtcccg aaatgttgat cgcgatcgaa
     1621 gcggcgatcg ccgaatcgcg ttccagaatg ccgaacgctt tcagcgcaac cgccatatcg
     1681 aaggcgtcgg tgaggccaag ctcctcggcg aatttgatga tctcgccagg actttggccc
     1741 ggctggaaac gggtcagcac ttcataatgg gagggtgtgc ctgtcttcag atcgacgatc
     1801 ggctcgaaaa cgagatcgaa cgcgctctgc gccaccgtgg cattgaaatc atgggcccgc
     1861 gccaaggtct cgtccatcag ccggtcgaac attcccgcaa gatcggcgtc ggtcacgctc
     1921 ttgatcttgc cttcggcaaa gcgcgtgacc gcgtagcgca acgccaagat gttctgttcc
     1981 ggcgtgagat tgcgcccctt gagcgagagc agcacctctt ccacctgaag gctctccaag
     2041 ccgcgttcgc gcgccgcgcc ccggatcttt tcggcgagat tgcgggcgcc gcgtggatcg
     2101 tcggtcacca cgccaaaacg ggtctccgaa agccgcgccg cgacgaccgc gcccatggtg
     2161 cggacattgg ctccgatacc ggccagcaga gcggaggcgt cttcgggcga cagcttggcg
     2221 caaacgtcgg gcagattggg cacgttcacc atggcgacgg cacccctgcc gcctgcgctc
     2281 tcggcagcgg ccgcaagaaa cgcatcattg tccgcgaggc cggtttgctt gtccttgccg
     2341 gccgtcagtc cgccgcgctt gcccggtttg accagcgcgc aggaaatacg atcgctctgg
     2401 ggcagaaagc acatggacag cgaagccttt tcgcctgacg ccaaaatcgt tggaagcggc
     2461 ccgatccggc cgccgggggc gagcccgcgg acaatggcgg caaagcgttg atgctcgctc
     2521 ggctgaaaca gctccgcggc ggagatccct tgaagttccg tttgggtggc gaacaggctc
     2581 accgcgccgg tggcgaacag gatggtccct tcccggtcga tttcgaaaag gaaatcggca
     2641 ctcgcaaagg caaaacccaa aaggcgaatc ggttcgatca tcacgccgca tccgaaggat
     2701 acgggtgctt ttgccatgag atcgattagg acttgatgaa ttaccgcggc ttaaccggcg
     2761 cttgctgcaa cggcacttac cgggccacaa cctccaaaag cgctcgtccg cgctcgtgcg
     2821 cgcgcagcct cagggcgtcg ccgaatgcgg cgaagatcgc ccggctgacc ggattctccc
     2881 catgccgcca ttcgggatgc cattgcacgc caaacaggaa cgccggcgcc cggtccatgc
     2941 tgacggcctc gatggccccg tccggcgcct cggcttcgac gcgcaaatca tgcgccagcc
     3001 gggcgatacc ctggccgtgc aatgagttga cctggaagct gtcgccgggc gcgagctcgg
     3061 gcagcagttc cgtcagcagg ccgccaggga cgacgcgcac atcgtgtatg ggcccatatt
     3121 gctcatcaag ggattctttg cggtcgcccg cccgatgatc ggcaaaaccc tcaacctcat
     3181 gcagatgctg atgcagcgtg cctccaaagg cgacgttcag ctcctgaaag ccgcggcaga
     3241 tgcagagcat gggaacgccc gcggcctcgg cggcgcgcat gagcggaatg gcggtcgcgt
     3301 cgcgcgcttc gtccagtggc gtatggcggc gcgcaggctc gccgccatag cgatgcggcg
     3361 cgacattcga ggtcgaaccg gtgaacagga gaccgtcgac gccggcgagg atgtctccga
     3421 ggtcgagagg tgggcgcagc gccggaatga ggaacggcaa cgcgccagag ccgtcgcgca
     3481 cggcctcgat gtatttttcg ccaaccatat gaaaggcgtg atcgccgact ttcttgcggt
     3541 cgcaaacaat gccgacgatc ggaagcttcg tgcgcatggc ttagatgtta gtgccgcgcg
     3601 gccgcgcaca gcaaggcgcg ggaaaattca gcggccactc tttttgaacg ggctcaaatc
     3661 ctcaatcacg aataggcgcg gtggtatgtc ggcggcctgg cgcatgtcgt tgagcaccac
     3721 catcgtacga gcaccctgtg catcgaccac ttcccattgc cgcaattcga gcctgccgcc
     3781 ggaatcggcg aaagtcagcg tgatgctccc cgacgccacc gcactcatct cgcgcgcggt
     3841 gacggatatt gcgcccggtt cgcgcttcaa cgcggaaacg cgggaatcgt ttgtgaggtc
     3901 gatgtcgtcg ctcaacaaga gtcgcaaggg cgaattggtc agcggatagc ggtcggtcgt
     3961 tttcagcgcg ctattctcga ccgcgaccgt gctgccgtcc gcaatgataa gattgggatt
     4021 gggtttctca tattcgaaac gcacccgtcc cggcttcttc agatagaagg cgccccgttc
     4081 ggtcttgcca tcggcatcca cctgcgtgaa gcgtccctgc acgctttgga tcgtgttgag
     4141 ataggccgaa acgcgcttca gatcggcctt ctcggctgag gagaaaacgg gcgcgatcgg
//
